LOCUS       DAEOSP010000001        56856 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.001, whole genome shotgun sequence.
ACCESSION   DAEOSP010000001 DAEOSP010000000
VERSION     DAEOSP010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.001"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(402..1403)
                     /gene="trpS"
                     /locus_tag="KRH54_000001"
     CDS             complement(402..1403)
                     /gene="trpS"
                     /locus_tag="KRH54_000001"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBG8577688.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(1426..2124)
                     /locus_tag="KRH54_000002"
     CDS             complement(1426..2124)
                     /locus_tag="KRH54_000002"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBG8577689.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     misc_binding    complement(2167..2407)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2498..3901)
                     /locus_tag="KRH54_000003"
     CDS             complement(2498..3901)
                     /locus_tag="KRH54_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG8577690.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     gene            4398..5474
                     /locus_tag="KRH54_000004"
     CDS             4398..5474
                     /locus_tag="KRH54_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG8577691.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(5591..6433)
                     /locus_tag="KRH54_000005"
     CDS             complement(5591..6433)
                     /locus_tag="KRH54_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG8577692.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKNVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            6617..7189
                     /locus_tag="KRH54_000006"
     CDS             6617..7189
                     /locus_tag="KRH54_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8577693.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(7352..7624)
                     /locus_tag="KRH54_000007"
     CDS             complement(7352..7624)
                     /locus_tag="KRH54_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577694.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDVHTHEHK"
     gene            complement(7751..8623)
                     /locus_tag="KRH54_000008"
     CDS             complement(7751..8623)
                     /locus_tag="KRH54_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG8577695.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(8919..12026)
                     /locus_tag="KRH54_000009"
     CDS             complement(8919..12026)
                     /locus_tag="KRH54_000009"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBG8577696.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(12119..12351)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(12526..13053)
                     /locus_tag="KRH54_000010"
     CDS             complement(12526..13053)
                     /locus_tag="KRH54_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBG8577697.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(13088..13873)
                     /locus_tag="KRH54_000011"
     CDS             complement(13088..13873)
                     /locus_tag="KRH54_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBG8577698.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(13883..14140)
                     /locus_tag="KRH54_000012"
     CDS             complement(13883..14140)
                     /locus_tag="KRH54_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBG8577699.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(14159..14617)
                     /locus_tag="KRH54_000013"
     CDS             complement(14159..14617)
                     /locus_tag="KRH54_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBG8577700.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(14657..15361)
                     /locus_tag="KRH54_000014"
     CDS             complement(14657..15361)
                     /locus_tag="KRH54_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBG8577701.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(15371..16006)
                     /locus_tag="KRH54_000015"
     CDS             complement(15371..16006)
                     /locus_tag="KRH54_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577702.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGSIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            16387..17427
                     /locus_tag="KRH54_000016"
     CDS             16387..17427
                     /locus_tag="KRH54_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8577703.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(17890..19245)
                     /locus_tag="KRH54_000017"
     CDS             complement(17890..19245)
                     /locus_tag="KRH54_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBG8577704.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(19271..19540)
                     /locus_tag="KRH54_000018"
     CDS             complement(19271..19540)
                     /locus_tag="KRH54_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG8577705.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(19830..21785)
                     /locus_tag="KRH54_000019"
     CDS             complement(19830..21785)
                     /locus_tag="KRH54_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577706.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNITGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKNIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLKDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIDVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGEDFNITGKFKTEMLNKEKIIINDDVV
                     PSLNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDDSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKSVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     TPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(21968..23443)
                     /gene="spoIVA"
                     /locus_tag="KRH54_000020"
     CDS             complement(21968..23443)
                     /gene="spoIVA"
                     /locus_tag="KRH54_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBG8577707.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(23615..24909)
                     /locus_tag="KRH54_000021"
                     /pseudo
     CDS             complement(23615..24909)
                     /locus_tag="KRH54_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(25093..26502)
                     /locus_tag="KRH54_000022"
     CDS             complement(25093..26502)
                     /locus_tag="KRH54_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG8577708.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGC"
     gene            complement(26554..27693)
                     /locus_tag="KRH54_000023"
     CDS             complement(26554..27693)
                     /locus_tag="KRH54_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG8577709.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            28012..28662
                     /locus_tag="KRH54_000024"
     CDS             28012..28662
                     /locus_tag="KRH54_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8577710.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(28819..29838)
                     /locus_tag="KRH54_000025"
     CDS             complement(28819..29838)
                     /locus_tag="KRH54_000025"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG8577711.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(29869..30483)
                     /gene="plsY"
                     /locus_tag="KRH54_000026"
     CDS             complement(29869..30483)
                     /gene="plsY"
                     /locus_tag="KRH54_000026"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBG8577712.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(30518..31837)
                     /gene="der"
                     /locus_tag="KRH54_000027"
     CDS             complement(30518..31837)
                     /gene="der"
                     /locus_tag="KRH54_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBG8577713.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(31871..33199)
                     /locus_tag="KRH54_000028"
     CDS             complement(31871..33199)
                     /locus_tag="KRH54_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBG8577714.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(33437..33637)
                     /locus_tag="KRH54_000029"
     CDS             complement(33437..33637)
                     /locus_tag="KRH54_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577715.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(33639..34571)
                     /locus_tag="KRH54_000030"
     CDS             complement(33639..34571)
                     /locus_tag="KRH54_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG8577716.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(34616..35548)
                     /locus_tag="KRH54_000031"
     CDS             complement(34616..35548)
                     /locus_tag="KRH54_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBG8577717.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(35603..36892)
                     /locus_tag="KRH54_000032"
     CDS             complement(35603..36892)
                     /locus_tag="KRH54_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8577718.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(36921..37613)
                     /locus_tag="KRH54_000033"
     CDS             complement(36921..37613)
                     /locus_tag="KRH54_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8577719.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(37628..38389)
                     /gene="pgeF"
                     /locus_tag="KRH54_000034"
     CDS             complement(37628..38389)
                     /gene="pgeF"
                     /locus_tag="KRH54_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBG8577720.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVTIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(38386..38847)
                     /gene="nrdR"
                     /locus_tag="KRH54_000035"
     CDS             complement(38386..38847)
                     /gene="nrdR"
                     /locus_tag="KRH54_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBG8577721.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(38869..39132)
                     /locus_tag="KRH54_000036"
     CDS             complement(38869..39132)
                     /locus_tag="KRH54_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG8577722.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(39236..40009)
                     /gene="sigG"
                     /locus_tag="KRH54_000037"
     CDS             complement(39236..40009)
                     /gene="sigG"
                     /locus_tag="KRH54_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBG8577723.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(40101..40823)
                     /gene="sigE"
                     /locus_tag="KRH54_000038"
     CDS             complement(40101..40823)
                     /gene="sigE"
                     /locus_tag="KRH54_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBG8577724.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGILGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(40848..41720)
                     /locus_tag="KRH54_000039"
     CDS             complement(40848..41720)
                     /locus_tag="KRH54_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBG8577725.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(42010..43284)
                     /locus_tag="KRH54_000040"
     CDS             complement(42010..43284)
                     /locus_tag="KRH54_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG8577726.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(43568..44725)
                     /gene="ftsZ"
                     /locus_tag="KRH54_000041"
     CDS             complement(43568..44725)
                     /gene="ftsZ"
                     /locus_tag="KRH54_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBG8577727.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(44915..45268)
                     /locus_tag="KRH54_000042"
     CDS             complement(44915..45268)
                     /locus_tag="KRH54_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBG8577728.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(45367..46041)
                     /locus_tag="KRH54_000043"
     CDS             complement(45367..46041)
                     /locus_tag="KRH54_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG8577729.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKKNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(46046..46744)
                     /locus_tag="KRH54_000044"
     CDS             complement(46046..46744)
                     /locus_tag="KRH54_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG8577730.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(46767..47507)
                     /locus_tag="KRH54_000045"
     CDS             complement(46767..47507)
                     /locus_tag="KRH54_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBG8577731.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(47777..49003)
                     /gene="murG"
                     /locus_tag="KRH54_000046"
     CDS             complement(47777..49003)
                     /gene="murG"
                     /locus_tag="KRH54_000046"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG8577732.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSASKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(49021..50151)
                     /gene="spoVE"
                     /locus_tag="KRH54_000047"
     CDS             complement(49021..50151)
                     /gene="spoVE"
                     /locus_tag="KRH54_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBG8577733.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(50203..51558)
                     /locus_tag="KRH54_000048"
     CDS             complement(50203..51558)
                     /locus_tag="KRH54_000048"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBG8577734.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(51577..52545)
                     /locus_tag="KRH54_000049"
     CDS             complement(51577..52545)
                     /locus_tag="KRH54_000049"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBG8577735.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(52586..53959)
                     /locus_tag="KRH54_000050"
     CDS             complement(52586..53959)
                     /locus_tag="KRH54_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBG8577736.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(54126..56105)
                     /locus_tag="KRH54_000051"
     CDS             complement(54126..56105)
                     /locus_tag="KRH54_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBG8577737.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(56117..56464)
                     /locus_tag="KRH54_000052"
     CDS             complement(56117..56464)
                     /locus_tag="KRH54_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577738.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
BASE COUNT        17766 a        10341 c         6154 g        22595 t
ORIGIN      
        1 ttaaaattaa ctcataaatt acatattggg tataaaatac aatgtatgtt agaagttgtt
       61 tcagaaggct atggttttct gattttttta tgctcagtag tgatatgcaa aaaagacaaa
      121 gttgacatca gttaactttt acctttattc tactttcccc aaagtaagaa taatctaacc
      181 cctttgagat gagcaaaagc tcatcttttt ttattatcaa aagatgctgt ctcaaaatag
      241 agataatcct ctttttcatt gttgaaatga ttaaaatata ttttatttca tacaaaaaag
      301 agtgcctcat gaacaaaagt tcactttgag actctctctt tcatatcata taccttttta
      361 ataactttaa aatttattta tcaagtaaag atagctaata attagtatct tctctctata
      421 aatccaactt ttttataaac ttttctaaga gtttttctag cttgtttttc ggctttttct
      481 gctcccatac tatatatttt ttctaaataa tctttattat taagaaggtc aacatatttt
      541 tctcttatag gtcttaaagc ttctgatact acttcagcca catcttcttt aaatatgcca
      601 taaccctttc cttcatacat gtttacaatc tcttctacac tctttttact taaattagaa
      661 tatatatcaa ttagattttt aatacctggt tgttcatcat tgtattttac aacacctaaa
      721 gaatcagtaa cacttctttt tatctttctc tttatagaat ctgaatcatc tgctaatagt
      781 ataaatgcat tttcatttga atcggattta ctcatttttt tagttggctc ttgcaaactc
      841 atcactcttg ctccaccttt tgggtagtat ccttctggaa ctacaaaagt aggtgaaaat
      901 ctattattaa atctatttgc taaatcacga gctaattcta aatgttgttt ttggtcatct
      961 ccaactggaa ctaggtcagt ttgatacaac aatatgtcag cagccataag aactggatat
     1021 gtgaataaac ctgcatttag atttgcttca ctcttttgtg atttatcttt gaattgagtc
     1081 attctactta attcacccat ataagtcata gtattcaata cccacataag ttcaacatgc
     1141 tctttgacat gtgattgtat aaatatagta tttttttctg ggtctaatcc acaagctata
     1201 tattgagcaa gtaattctat agtattggct cttaaaactt tagggtcttg aggtactgtt
     1261 attgcatgca aatcaactat actataataa caatcataat tgtcttgaag ttcagtccag
     1321 ttttttatag ctcctaaata atttcctaaa gtcattttgc ctgatggctg tgcaccacta
     1381 aatataactt ttttttcact catttttttg tacctcctta tatatttata ttgattttaa
     1441 catatttaaa actatgtctt tagagttatt ggcagcatca tgtacaaata catcataagt
     1501 aacatcagca cttccatctg ccttatcaga cattgctctt attattacaa atggagtatt
     1561 atttaaatag catacatgag ctatagctcc accttccatt tctccacaat atccaccaaa
     1621 atcatttact aattcttctt ttaattcttt agaatttata aatttatctc ctgtaactac
     1681 tcttcctttt aatactttat gagtctttac ttcttccaca gaagatttgt aagcagcatc
     1741 tataagtctt tcatctgctt taaatacaga agtcttcatt cttggtataa ctcccttagg
     1801 gtctccaaat gctgtagtgt ctacatcata ctgtatagca tcagttgata ttactatatc
     1861 attaacatca agtttttcgt ttaaagctcc tgcaacacct gtattaacaa tagcatcaac
     1921 cttaaattcg cttattagta tctgggcaca aagagcagag ttaaccttac ctatgccaca
     1981 ctttactaac actacattct taccttccaa aattccctta taaaattcta agctagcttt
     2041 ttttatagtc tctctaatat ccattaaatc aactaaaata ctaacttctt catccattgc
     2101 acctataatt cctattacgt tcatttttcc tcctaaattt tctatattaa aaaatccgtc
     2161 ctaaatcaat aggacggata tattttccgt ggtaccacct aaatttatat acactcaaaa
     2221 ttgaatatat atatcttaat atatgctata acgtgcatac acgatatcaa atactctgta
     2281 atattaaatt acatttcatt ttactcctca aagacccatt caataaatac tttttattaa
     2341 gttctcagca tccttaattc tctgtgcaaa aatatattta tttactattt cttatctcag
     2401 gatttaattt attaatttct ttattttata atattcctta cactataaat tgtcaactat
     2461 atattataat atccaaaaga aatttttaaa atacatttta tttagctgtt ttcttttcat
     2521 atctattcca taaccataga ccagataata taactagaag acctccacca atcatctcta
     2581 tctcatatat aacattttca gcaaatgtag gcataactgg tgctgcagat aataatatac
     2641 ccactacagt tacaactaaa accatttttg ctaaacctag gcaagtactt gttgattttg
     2701 ccatagtata aggtctaatt tcatctggtc tctcttttct tagtttaata taagaagcat
     2761 ataacaatac atatgggaat aatgcagtta atgctgtcat agttacaaga acattgtata
     2821 tagcatcaac agaaggcatt aatgtagtca ccaatagtat tatagtaact attattgctt
     2881 gaagtataac tgctttagaa ggtatcttgt gttcatttac ctctactaaa ctatctggaa
     2941 atattccttt tttaacacta ccaaataaca tttttattgg tgatgctatg taaagaacta
     3001 ttgctcctaa tactgaaaaa gctataccaa aagctattaa ttgaaccaac caagaaccaa
     3061 ttcctaaaga cgcacaaaca gcagaaagtg catctaatac acctttagaa gcagtaattt
     3121 ggtctggtgg tattatcatt gttattgcaa ttgagcctaa tacatataat gaagctacta
     3181 ttccagctga tattaatatt gccttaggga agttcttttc aggcttatct atttctgtta
     3241 tgaagttagc agcagtctct gctcctgcta aaccaaacat tatagctgat atagcaacaa
     3301 atgcatccat attaagtttt ggagtcattg aagctattgt gtatgtagaa gctatatcat
     3361 gttttttcaa cacaaatact gctacaaatg aaaatattat aagtatagca cttggtattg
     3421 ttgaacctaa tgcacctgta ttagtaaata tttttgtaaa acttattcct ttagttgcta
     3481 taagagataa tgcccagaat attattaaag ataatataag tacaaacatc ttattatcag
     3541 caagggctgg atttcctatt gtataagaaa catttactgc taagaaagtt aaaaatgaag
     3601 aataccaaaa tattttagct gtccagttta accaagaaac taaaaatcca tacttttctc
     3661 caaaggcttc cttaacccaa tcgtaaagac ctccatcacc tttttgatat gtagaagcta
     3721 attcagcaca aattaaagct cctggtacaa agtatataaa tgagaataat aaccacatag
     3781 gtattgcacc taatcctaaa ccaaagcttg atgctgtaga tttagctatc catcttatac
     3841 catataaagc tgatacattc ataaatacca aactccatag gcctattttt ttcttttcca
     3901 tcctaattta atttcccctc tctattaatt attttaaatt taatttcaaa attaataaaa
     3961 tagattcatt attattaaac tgatattttt caaaatattt gcatattcaa aaacagtttt
     4021 aataataaac cttaaatact ttgaaatcaa atcttattaa aaatgataaa tttaaaagtt
     4081 ttaccacttt aattctaatg atactaaatt tttaaaattg tttcaatatt attttattat
     4141 aaaattttat ttattatttt tcattagtat ataaaaagat aaaatacata actttataga
     4201 cttaatttat tacttttata gaattttcac aataaaaagt cattttactt ccgtatttaa
     4261 tgcaaaagta gagattaaac ttaatataaa aaatatataa caaatttttt ttatatttct
     4321 agaagaaaag tatactcttt tttgtttaat aatgctaaaa ttgatttgta ttgaattttg
     4381 attaggagga aattattatg aaagctcaaa gattaaatgc tgtacttgaa caaatgaaaa
     4441 aagatgatat ttctcaaatg ttagtaagtg accctacttc tatattttat cttacaggag
     4501 tactaatcca tccaggcgaa agactattag cattatactt aaatttaaat ggcaataata
     4561 aattatttat aaatgaatta ttcccagttt cagaagattt aggtgttgaa atggtttggt
     4621 ttaatgatac tcaaaatcca gtagaaataa taactgaaca tatagacaaa aatgctacta
     4681 tgggtgttga taaaaactgg ccagctagat tcttattaaa cttaatagaa ttaggtggag
     4741 gaagtaagtt tgtaaattca tcttatataa tagatacttt aagaatgtgt aaagatgaag
     4801 aagaaaaaga gttaatgaga atagcttcaa aattaaatga caaggctatg gaacaactta
     4861 aagctactgt ttctggtgaa cttacagaaa aacaacttgt tggtaaatta tctaaaatat
     4921 atgaagacct aggtactgat ggattttcat ttgaccctat tataggtttt ggacctaatg
     4981 gagctaatcc acatggtgaa cctggaaatg cacttgtgaa accaggagat gcaataatct
     5041 tagatatagg ttgtataaaa gataactatt gtgctgatat gacaagaact gtattctata
     5101 aagaaatacc tgaaaaaggt agagaaatat ttgaaatagt tcttgaagct aataagagag
     5161 ctgaagctat tgtaaaacca ggtgttagat tctgtgatat agatgctgct gctcgtgact
     5221 acataactga aaagggatat ggacagtact tcactcatag aactggtcac tctataggac
     5281 ttgaagttca tgataaagga gatgtatctt ctataaatac agatactgta caaccaggaa
     5341 tgatattctc tatagagcca ggtatatatt taccaggtga gttcggagtt agaatagaag
     5401 atttagtact tgttactgaa gatggttgtg agatattaaa caagcatgac aaagaaatat
     5461 gtgtagttgg ttaattctaa aatcaatttg ataaaattaa aaagccatat aaaaatgttt
     5521 taaacatttt tatatggctt taatttctca atctattatt tatttcaatc cattatttat
     5581 attgcagtat ttatattgca gttattaaga ttgcaccctt tgaagtatat gtccccatca
     5641 ctgaaccaat ttcacagatt acaacatctt tgtattttac attcttttcc ataacttccc
     5701 ttaccttttg agccttttct gggcaattag catgtcctat aaataaaacc ttttcttctt
     5761 tatctttaat ttttgactct actaaagtaa tcagcttttt caaactgttt ccttcacctc
     5821 ttgccttatc tataggagtt acaacgccat ctgcaacttg tattatcgcc ttaaaattta
     5881 aggtatttat aatttttcct gctattggat ttattctacc acccttaata gcattttcta
     5941 aagtttcaag tgttccaaaa aatggaggat tatctttata tttatttatt attgaaacaa
     6001 tctcttcaat ttcttttcct tcttttatta atattgctgc ttttaacact aattgtccta
     6061 ttccaattga cccactaagt gaatctataa cctcaacttt cttgttattt ccttcctttt
     6121 caaacatatc ttttgctaaa agtgcactat tataagtccc agataatcct gaacttatat
     6181 taatcactaa tacactttct tcctgacaat ggtatgcttc taaaaattta tcaggagaag
     6241 gacatgatgt ttttggtagt actttactct cactcatttt tctatagaaa gtttcattat
     6301 ctatttcctt tccagatata taactttctt ctccaaaaga tacatttaaa ccaacaactt
     6361 ctatgttgta tttttctatt atatcatcag gtaagtcaca tgcactgtcc actatcaatt
     6421 ttattttatc catattaacc tcactataag atattatttt atttattcag tcggtcgtac
     6481 gaccgattta attattattt tatatattga atgaaaaaaa gtcaatagat atagattctc
     6541 gctttattga caataaatat ccataagctt ataatgttat taatttgtta taaatataca
     6601 tggaaaggta ggttttatga aaacaaataa tatgaaacag gctattttat catctgcaac
     6661 caatttaata gccaaaaatg gagttcaaaa tactagcctt gctgatattg caaaagatgt
     6721 aaatataagt aaaggtactt tgtattatca ctacgcatct aaagatgata ttatatatga
     6781 tattgcggat actcatttag aagttataac tagtgctata ctaaactgtg taaaaaatgt
     6841 caaatcaaaa aattctcaaa tagaaatggt taacttaatc ttagagaaaa tatctactat
     6901 agaaagtcgt ggtagggtac atatgtatct tatttgtgag gcaattactt caaataatga
     6961 tttaaaggaa agaataagat taaaatatat tgaatggaga actactctta aatctgaaat
     7021 tgttgaatca ctagaaaaat ataatagtag cgaaaatgaa caggatgctg aatcattttc
     7081 ttttttactc atgtcaatag ttgatggttt agttgtccaa agtttattaa aaacagaaaa
     7141 aataccttat gaaaatatag cttctttttt agttaatcat tggatttaaa cttaatactt
     7201 agataattaa taaaagtgaa ctatttaagt tatctacttt aacagttcac tttcttattt
     7261 attctcaata atttttactt aaaacattca atttttattt aaaccttcat ggaatcacaa
     7321 aataaaaatc tcttataaat ttaaaatgtt tttatttatg ttcatgtgta tgcacatctg
     7381 aatgttcatg accatgtgta tgtgtatgct catggctatg ctcatgagta cctgaaaaac
     7441 catcatcatg attatggtcg tgacctacac catttacatg ctcatgttca tgcccatgac
     7501 tgtgagaatg ttgatgtgaa tgttcatggt tatctcctcc acagcatcct tctcctccag
     7561 tcccacaaca cccacttgta tgtggattat gatgatttct tactacaaat ctctctctta
     7621 acataattct tatacctcct agtatattat tgagtacttc atgtacccca ttaactacag
     7681 tatacatcta actaggtata atctcaatac atatggcatt ttttaacata tataattaac
     7741 atatataatc ttaaagaaat ttatccttaa aacctccacc ttttacttct tcaacgttat
     7801 tgacagaaat aaaagcttca ctatccatat cataaattaa atctttaaat ttacctattt
     7861 catttgcaga tattatgcaa taaatcatct ttttcttatt ttgtgtatat gcaccttctg
     7921 cctccaaaaa agtgactcct ctacccattt tattcattat cactttggat atttcatcat
     7981 atttatttga tattaaaagt atagattttt ttctatcaaa gcaatctttt cctatatcca
     8041 tagtaaaaga aattatatac attgtaatta aagtgtacat caccaattta gctccaaata
     8101 aaaaagctcc tgcacatact acaaaaaaat taataaataa aaatgtattt ttcataggta
     8161 tatcatactt tcttttcatt atggctgcta ttatatcaag tcctccagca gtagctcttg
     8221 ctttaaaagt taaccccata cctataccag ttaatgcacc accataaata cactgtagca
     8281 tagtatcatc tattgggaaa tatttagcaa tatcttgtgt tagccccagt gctagagaaa
     8341 atagtaacat attgacaaaa cttattagca aaaatttatt atcaaaatac tttctagcta
     8401 taataaatat tggtatgttc atcaagaaac ttgatattcc ctgattaata ccaaataatt
     8461 tgtataatat aacacaaatt ccagctactc ctccacttaa taacttggct ggtgctagat
     8521 ataaatttat tcctaaggtt gatagaatca tacccaatat aataagacaa tattgtaaaa
     8581 atatattatt atcaatactt agctttttac tttcaccata catcaaaacg cacccctttg
     8641 cataataaat aaaccaccct gcataacaca cgataaattt acaaataaat ttatcagaag
     8701 tagtttccta aaaatattat tcatcctcac ttaaacgttt attcattaat attctatcac
     8761 atgacataaa taatttatat gatttatata gatttattta ttgtaaattt tggaatatta
     8821 aaacatttta ttgaatagat aattatatta cataaaaaat ccctatagaa atctataaac
     8881 taatttctat agggattttg gttaactaac tattattttt atttttttat aacttttata
     8941 cctgtcatat ggtcatttaa gttttgttcc tctaaagatt tatcatctac tctgtttatt
     9001 tctaaagcta aagtttcaga cttgatatat tcattaaaat ggtcaacagc cttagcaatt
     9061 tcatcatcac cacagtattc tattactata ttatctaata cttcaaagtt attagatttt
     9121 cttaattgct gtactttaga tataaattct ctagcataac cttcattaat caactcttca
     9181 gttagcttag tatctagtat aacaaaaaga ttgttttcaa cagatacatt aaatccttcc
     9241 ttagcagtta tacctattaa tactaaatcc ttattaaatt caaaagcttc accatctaaa
     9301 tcaactgtta aagtctcacc tttttcaagt ttattagcag tttcatgagc atttaattta
     9361 cttaatgctc caacgaataa attcatctta gaacctaata atggccctgc ttccttaaag
     9421 tttggtttta aactaaagtt catatactca tctaaatcct tagcaaatat tacctcttta
     9481 acattaagct cttcttttat taaatcaact acatcactta ttgtatcttc aaacttacca
     9541 tctactaaca ctttttgtaa tggttgacga acttttattc tttctacttc tcttgaagct
     9601 ctacctaaag taactaagtt tttagctaaa tccatttttt cttctaattt agtatctact
     9661 aactcttcat tacatttagg atattcagct aaatgaacag atacttctcc tgttaagttt
     9721 ctatacattt cttctgacat atatggtgca aatggagcta tagctctaca aagaccatgt
     9781 aacaattcat aagttgtatt gtacactgct tttttatctt ctgttaattc agttgcccag
     9841 aatcttcttc ttgaacgtct tatataccag ttagataaat catcatttat aaaatcttgt
     9901 atcattctta aagttttatt taactcaaat atagctaaat tttcttctac atctttcatt
     9961 aaattattga acttagataa tatccatcta tcaagttctg gtctatcttt atattctaca
    10021 aaaaattcag ttggatttat atcatctgtg tttgcatata aagtaaagaa gttatataca
    10081 ttcttcatag tacctataaa tttactttgg atttctttta atccatccat atcaaattta
    10141 gttggagtcc atggtggaga aacatatagt aaataccatc ttaatgcatc tgctccatat
    10201 tggtcaaata attccattgg atttactgta tttcctcttg atttactcat tttcttacca
    10261 tctttatcaa gtacaaggtc tggaactaaa acactcttat atggtgcttt tcctaataca
    10321 aatgttgata ctgcaagtaa tgagtagaac caacctctag tctggtctat accttctgat
    10381 atatagtcag ctgggaacaa ttggtcaaag ttttccttat tttcaaatgg atagtggtgt
    10441 tgagcaaatg gcatagcccc actatcaaac caacagtcta taacctctgt tactcttgtc
    10501 attggttttc cacatttttc acattttaag tgtatatcat ctacatatgg tctatgaagt
    10561 tcaactgttt ctggatttac atcttctata gctttttcag ctaattcagc tcttgaacca
    10621 actgaatctg tatgaccaca ttcacatctc catataggaa gtggagttcc ccaatatcta
    10681 gttcttgata ttgcccagtc atttagattt tctaaccagt ttccaaatct cttctctcct
    10741 acaaaacttg ggaaccattt tacaccatta ttattttcta ttaatttatc tttaagttta
    10801 gtcatctcta tataccagct tggttttgca tagtatacta atggagtttg acatctccag
    10861 cagtgtggat aattatgaac tactttttct ttgctatata atttattttc agaagctagc
    10921 cattttatta tttcaacatc tagcccatct tccattacaa atctaccttc ccaaggagta
    10981 gatgtaaatt taccagtttc atctactggt tgaataactg gtaaatcata tcttttacct
    11041 gtgttgtagt catcttcacc aaatgctggt gctgtatgaa ctataccagt tccatcttca
    11101 gtagttacat aatctcctac tgtaataaaa aatgcttttt tgtcaacttc tacaaaaggc
    11161 attaattgtt catattctaa accttctagg tctttacctt tcatttcttc taaaacttca
    11221 taatcttcac ctagtacttt atcagctaaa ggtttagata cataatatac ctcatcacct
    11281 tttttaactt ttaaataatc tacatcagca tttactgtta atgaaacatt tgatggtaaa
    11341 gtccatggag ttgttgtcca tgctaagaag tattcatcag catctttttt cttaaattta
    11401 gctataactg tattagtttt tacttcttta tatccttgtg caacctcatg actagcaaga
    11461 ccagttccac atcttgggca ataaggaagt attttatgcc cttcatatat atatccttct
    11521 ttgttgaatt tgtttagtat ccaccataca gactctatat agttattatc taaagttata
    11581 tatggattat ctaaatcaac ttcataagcc attctctcag tcattattct ccattgtttt
    11641 tcaaatgaga aaactgactc tctacatttt tggttaaatt cagctattcc atattcttct
    11701 atttgttgct tactagtaag tcctaactct ttctctactt gtatttcaac tggtaatcca
    11761 tgtgtatccc aaccagcttt tctctttact tgataaccag acattgtttt atatctacaa
    11821 actgaatctt taagcgttct agataaaaca tggtgtactc caggatttcc attagctgtt
    11881 ggaggtcctt catagaatac gaaacttggg tcatctttcc ctttttctaa agttttttca
    11941 agtatattta tgtctttcca ataatcaaaa acttgtgctt ctgcttgttt tacagaactg
    12001 tctaccaatg gcttaaattt cgccatgtaa atcacccttt cctactaaat attataattc
    12061 caaaattttt tattctttca cataaattta aatactaaaa aactcgcccc ctttaaacca
    12121 aagggacgag ttataaatcg cgttaccacc ctaattctat atgtaaacta aatactagct
    12181 acatataaca ctctaatagt ataacggtac taaccggcac agcttagtag aatttaatcg
    12241 ttcagcctgc agctcaagag tgatttttat ctttataaat tcattggttc tcagcatacc
    12301 caattctctg taaaattatc taaagataaa tgtctctatc aacgctttta aaaatttatc
    12361 tgtgtttata tgaaattcta taacaaataa tttgaatagt caatatattt ttgaaaaata
    12421 taataatttc tatttttctt acatttttat cctattttta atttttgtta tagatttctc
    12481 aaactcacac aattattcta cataaaaaat aaacaaatta caagcttatt ctaaagttgt
    12541 agcagcttca tcattactat aattatacac tgttgttcct tcaaatgcgt ttacacattt
    12601 ttcatctaca tcataaaaaa tctcatcaac acttcttatt tcatcttcta acagtgattt
    12661 aaatttattt ctaaatatct taaattcttt gactaaagca tcatactctt ttcttatctc
    12721 tataacacgg ttattagctt gttctataat ttgtctagct tttaaatcag cttcttcaac
    12781 aattatctta gcctttttat tagccgcgct acaagtgtct tctgctgcac tttgagcagt
    12841 tattaaagta gcttttagtg tttcttcaat attttcatac ttgtttactt ggtcttgata
    12901 taaccctaat ttttctttaa gagctgtatt ttccctacat agagattcat aatcttcctt
    12961 gatgatatct aaaaattcat caacctcttc atctctatac cctcttaaac cttttttaaa
    13021 ctctttattt tctatctcaa ttggagttag cattacattt cctcccagct ataatttaaa
    13081 ttagtcctta tacaattaat cttgcttgaa cttttatttt tcctttttta gtaatatctc
    13141 ctatttgtgt taccttagct cttcctttgc ctctaataga aatcaaatca tcgtttttta
    13201 ctattttaga agttgaagtt atcttttcat aattgacatg gactttttct ccatttatgt
    13261 attttgcact atcttgtcta gatatattgt aaataccact tatcacacaa tctaacctat
    13321 ctgaagatac tgtaaatgat acttctttat attttgaaga actattaaca atattttctc
    13381 tggaaatttc ctttacaatt acattatttc ttgaaacctt ttctaagttt acaattataa
    13441 aatcgcaaat atcactactt acaactactt gacaaaagct atcatgtata attatatctc
    13501 caattttttc tctttttata cctagaccca gtattgaccc taagtaatct ttatgagata
    13561 tatttttaaa tttgaaattc ccttctattt gtaaaaatct aagagtatct tctatatctt
    13621 catattccat gtaaaatgga taaataaata cagtgcttct ttcagcttgt tcataacctc
    13681 catccacact gtattttatt tcatttgtag aatttaatat tgctacagca tttttcactt
    13741 catatggatt aagaaactcc gtagacttaa catcatagtt ttttatacaa ctattagcct
    13801 tgtctatcac tttaaacatt ttatttttca agtctatatc ctttatgtga tttgttagtt
    13861 ttaatttatc catcctatca ccttagaata taaccaatac taattgtctt aggaacatca
    13921 ataatactat tgcaattaaa ggagaaaaat ctataggtcc atttgtatat ttatacatta
    13981 tagaccttat tggcccttct attggctctg taagattttc caaaatatca aaaattttac
    14041 tttgtcttcc atttggaaac caagtcatca agctttttat aactatcatc catgctatta
    14101 tatctaaaag ataataaagt gcaattctta ttgttcccat atattaaccg cctttttact
    14161 atttattttg ccaagggaag aatgctttac tttctaattc ttttttcata tttgcatcta
    14221 tatccacatt attaggagct agtataaata ttgatttaga aaccttttgt atacttccat
    14281 ctaagacata aactgcccca ttcataaagt taaatataga tttgcgaact tcagaattag
    14341 tcaatccttc caaattcact ataactgccc ttctttgttt tagatggtct gctatagttg
    14401 cagcttcatc ataaacctta ggttcaacta ttacaacttt catttggcta gttgtatgta
    14461 aatttacaat tttatttgct tttgcagtac taaagccact gttcatttct tcttcttgaa
    14521 ctatatcgtc catgccactt tcatattcat cttcaatgta atcttcttct tcatcaacta
    14581 tccaattctt aaatttagat attattccat ttgacattat taacttcctc cttaaaattt
    14641 attcaaattt gagtaattat tttttattgt agtttctttc tccaaagatt gatgttccaa
    14701 ctctaacaat ggtagcccct tcctctatag caatttcata atcgtgactc attcccattg
    14761 acaaaacatc catccctaca ttagggatgt ttagttcttt tatttgtttt gataaatctt
    14821 taagaccttt aaatacacct cttatctcat cttcatcttc tataaaaggt gccattgtca
    14881 ttagaccctt tatttttata tttttatatt tttctgacac ttctttgaca aagtctacaa
    14941 cttcttctct atcaagtcca tgcttagact cttctttaga tatattcact tgtactaagc
    15001 aatctatttc tctgtttatt tgttctgcac gtttttgaat ttcttcgcat aatgcaattc
    15061 tatctaaaga gtgtatcata caaactttat cgattatgta cttaacttta ttggtttgca
    15121 aagttcctat caggtgccag tttaatttat cacctataat atcatacttt ctagctaatt
    15181 cttggggttt attttcacca acagaagtga ttccttccct cattgcctca ttaactaagt
    15241 ctacatcaac agtctttgtg actgccaaaa gggtaatctc tttgctgtct ctatctactt
    15301 tttttgcagc tttatcaatt ttgtttctta tctcagacaa attatcttta atagaaccca
    15361 ttattatcac ctactttatc cttatatttg tgtttatact gttaggtctt aaaattattt
    15421 tgtcatatag attaacactt gtaactccat ttatgtcatt tttataataa tccacaaata
    15481 cagaagattc attctcataa gttattcctt ctaatatgac aaattttaca ctttgtgatt
    15541 gttcatcaat tacatataca ccacgtttat tgtcttttgt ttttatagaa gttttaggta
    15601 tttttaaaca ttccatctga ttatatatta tatcaaattc ttcgacactt gtatcctgat
    15661 ttgcaacaat ttggttactt atttttaatt ttaccattat tttattatta ttttcatata
    15721 ttttgtaaac ttttgcatct aatttttgct catgatattc aattattaca ttttgatttt
    15781 tttcaaagtt attagaatct tctttactga tgtatgtaga tagataatta ctctggtcat
    15841 tggcatcttt ttctatactt ccttttacat gaatctttgc atcaactttt tcatttttta
    15901 acaccaacgt atctacattt ttgctaatta tcattactaa tatattaaat actaaatata
    15961 taagaatacc aagtatcact aatctggtat attttacctt tttcaaagac tctcccccta
    16021 taaaattact ttcattataa gtacttcttc taaaaaatgt ttattccttt tttataagct
    16081 aatattttac gttctaataa ttattttttc catattaaat ttatttttta tcaatcttcc
    16141 tatacaagac tgcattattt atatataatt gtattataaa gtataagttt aatttacatt
    16201 ttatacgtta agcttacttt atatgttttt atagttattt ttatttttag atgcaatata
    16261 aaaccaatta agatttaaaa taacttttgt tatgttgaca tttatttagg taatcattta
    16321 tgaattagga taacgttatc ataacaaaag tttatgtcct aaaaaaaata caaggaggat
    16381 tttcttatgt cagaaatttt attaggaaca ggtttacttt tattggttct atgttgtttc
    16441 acaattttta gttacaaggc cccatatggt atgaaggcta tgggagctct agcaaacgct
    16501 gcatgtgcta ctttcttagt agaggcattc aacctttctt tacttggtga tgttttaggt
    16561 atggaatttt ttagaagtgt tggagctgct aatggtagtc ttggtggagt tgctgctgct
    16621 atacttgtgc ctttagcctt aggtgtatct ccagtttatg cagttttaat aggattatct
    16681 tgtttagatt tttctatatt atctggattc gttgctggat actttgtttc tttcttaatt
    16741 aagtttatag agaaaaaagt gccacctgga gtggatttag tggttgtaat tcttctatgt
    16801 gcaccgctat ctcgtcttat agcatcaaat gtagacccat tagttaatag tacactacta
    16861 caaattggac aagttttgat agaagctacc aacattagcc ctataataat gggaattata
    16921 ctaggaggtc taataacagt agttggaacg tctccactta gttcaatggc tcttacatct
    16981 ataattggtc ttactggtct tcctatggca ataggagcat taggtgcttt tggttcagct
    17041 tttatgaact caacactatt tgcaaaattg aaattaggtt ctaaaaaaga tattatatct
    17101 gttgctattg aaccattaac ccaatcagac ataatttcag ctaatccaat acctatatat
    17161 tctactaatt tctttggagg agccttttct ggtatcattg tagccttgat gggtcttgta
    17221 aacatgacac caggtacagc tacacctgtg gctggttttg ctataatgtt tgcttataat
    17281 ccagttcaac aagttcttat aacagctggt ttatgtgctt tattcaatgt aattgcagga
    17341 tttttaggtt caaaaatatt cagtaccttt aaagttctta ctgctggtga aatacgtggt
    17401 actgatgtag aagaagaaat tgcttaaaaa acaataagat ttataaattt atatctcaag
    17461 tagtagagtt ttatattgta ggcaatttta ttaattgcaa acgttttatt ttttatttat
    17521 gtttatgatt attatgtgtg ttgaatattt agataaattt ttatttatta tttttattta
    17581 gatttttgtt tagtttttta tttggttgat atattaggaa taaaaattag tatagtttat
    17641 tactagttaa gtagcataac tttttagata cataaaaaat tttatattaa ttagatatta
    17701 ttaaaataaa agcgatacat gaatttttaa atgtccatgt atcgctttat ttttgccata
    17761 atcttaacaa ctgattttta aagtttatta tcttaacaaa gttcattatc ttatataata
    17821 tttaaatgca taggcttctt taatttaacc atctaaatat ttaattaaat attattatat
    17881 gtaatataac taatttttaa atgaatgtat aggagctgga atccttcctc ctctttgagt
    17941 aaataattca gatgaatttg tatttatagg cattactggt gctcgtccta aaagtcctcc
    18001 aaattctacc atatcaccta catcacatcc tggggctggt ataattctaa cggctgtagt
    18061 cttattatta atcataccta ttgctgcctc atcagctatc atggctccaa gcgtacctgc
    18121 tgtagtattt cctggcacag ctatcatatc aagacctaca gaacaaatag cagtcattgc
    18181 ttcaagctta tctatactta aagcaccttt aataacagca tcaatcatac ctgcatcttc
    18241 agatactggt ataaacgctc cactaagtcc tccaacatgt gaacatgcca ttactccacc
    18301 ttttttaaca gcatcattta aaagtgctaa tgctgctgta gtcccatgag taccaacaac
    18361 ttcaagaccc atttcctcta atatatttgc taccgaatca ccaatagcag gtgtaggagc
    18421 tagtgataag tctattatcc caaatggaac atccaatcta ctagaagctt cctttgccac
    18481 aagttgtcca actcttgtaa ttttaaatgc agtcttttta actgtttctg aaacaacatc
    18541 aaaaggctct ccctttactt tctctaaagc tctcttaaca acaccagggc cacttacacc
    18601 aacatttata attctatcag cctcaccaac accatggaaa gctcctgcca taaatggatt
    18661 atcttctact gcattacaaa atactactaa ttttgcacaa ccaaaacctt ttgcatcttt
    18721 tgttaattct gcagtttctt ttatgatttc tcccatttct ttcactgcat ccatatttat
    18781 accacatctt gtagaaccta catttactga agcacatact ttattagtac ttgctagagc
    18841 ttttggaatt gacttaatta gtatcttatc accttttgta tatccctttt gaactaatgc
    18901 tgagaatcca ccaataaaat ctatacctaa atcctctgca gccttatcca atgtctgtgc
    18961 aaactttaca tagtcatttt catctgttgc acctgcaata atggaaattg gagttactga
    19021 taccctttta tttacaattg ggataccaaa ttcacgctct atatcttttc caaccttcac
    19081 caaatcttta gcagaactca taatcttatt gtatattttt tctctagctt tatctccatc
    19141 agggtctata caatctaata aagaaatccc catagttata gttcttatat ccaactgttc
    19201 ttcatctatc atctttattg tttcaagtat atttcctaca ttcactatta tcacctcccc
    19261 ttactgtcta ctacagatta tgcattgaat taaatatttc ttctcgctga atttttacat
    19321 ccactgataa tttcttacct ctttcaacca agtcctcaac cgttttctca aaagaattta
    19381 atgatttctc taaatcaact atcataatca tagtaaaata atcctgtaat attttttgtg
    19441 atatatctac tatgttgata ttttgcttgt ataattcatc actaattcct gccactatac
    19501 ctggtttatc ctttccaatt actgttaaaa ttgctttcat aataatatcc tcccttattt
    19561 ttttataaat cttatatttt tacaaattga actatgtgtt aacatatatt tcatttattt
    19621 gttagtaaac cactttttat gtatatattt aatatatttt aatacctata cattcatttg
    19681 tcaatttttt ttcaatattc tattattttt atactattta gaaaattcat atattatata
    19741 tgttttctca acaataaata cagttttcat aataatgaaa ggtattactt agcaatctca
    19801 tgattttcta aataatacct ttctaaaaat tattttttta attttatgaa tttaattata
    19861 ctttttctag tatcaggatt tacaattaat atagaaccaa tcataattgg tgttagtata
    19921 caaaataaaa aaataatatc tttttttaga ttatattctt tagattctaa tttataatta
    19981 ttatttatta ttccatcata atcacttata tttgacttat ttttatttgt atcttgattt
    20041 ttgttagaaa tactttccct ataaggaaaa gaatgttcat tggtaccaaa tactgatttt
    20101 gagcttaaat cataattatc atcattttct gtaattgaat ttttattgta aaatcctttt
    20161 tctttatcag aattattttc tttattaccg tctttaggta tatcatgagg atttttattt
    20221 tcattgctat cagtacttga ttctacatca tctttaggag tatttggttg tttctcatca
    20281 gaatcatcat tttcattatt attattttca ttattattgt tttgattgtt attactatca
    20341 ttattttcat ttccattatt cccatcttga ttgttatcat cctcatctct atccgggtcg
    20401 tctctacctc ctcctccacg gtcaccatca atatcatcat tttcccgttc atcatctggc
    20461 gtctcttggt tgtctccgcc tcccattata aatcctggtt taaaaactat atttgaatac
    20521 ccttctttta atcctcctac aacttttact ctaaaacaac gcaacttaaa atcttcaaaa
    20581 aaaagtatag cttgatctct aatatcctcc tgctcttcag atatccaatt gattccatcg
    20641 cttgaatatt caacaaaaac cttacttgca ctatacggca tatcataaaa taaaacagct
    20701 acatatccat tttttgtatt ttgaaattcc agttccaaat tgtctattgg ttttttctct
    20761 ataacagata cattcaaact aggaactaca tcatcattta ttataatttt ttctttattt
    20821 aacatttctg ttttaaactt tcctgttata ttaaaatctt ctcccttttc caacttttca
    20881 taataatcag taaaatccca ttctacatct atatcaagtc gattaccact agatgtttct
    20941 aataccatat atttgggaaa ttttatattt tctattttat catctttact tacttttatt
    21001 tcatcactaa atacatcaat tcccctatga caacatttca caatatcata gccagaatct
    21061 tcagctatag agggtataag ctcattataa tcatcaatta tataaacttt cttactttct
    21121 gatataattg attgtgccaa cttattatta tcttttagta acccactttc attgttgcta
    21181 ctatatgctt ttattgaagt ttcttctatc tcaacatcac ctttagaata aacacctata
    21241 gcatgttctc cattcacttc aatattctta tttagtattc taatatcatt ttcagtataa
    21301 attcctattg catcttttcc atttgccttt attttttcaa cagattcgta ggatgattta
    21361 atatgtaagt ctcctccttc ttcaacgtat aaggctgtcc catcagtagc aattatgttg
    21421 tttataccaa tacttatact tccaccattt tctatatgta ttactccttc ttttcctcct
    21481 tcacctgtta tattcaattc attatcatca ataataaatc tcccatgatt ttttatcaat
    21541 attttatatt tgttggtatc tattgtaaaa ttactgtcat aaggaatata aaatctaaac
    21601 tcttcatcta ttaccatatc acaagaaaga gtataaacta tttcttcttt attattttta
    21661 ttttcttgaa cccacctttc aaaatcttca agacaatttt cctcttgtcc aaaaacttta
    21721 tgtggaaaac ttataaaaca caaaactatt atactcataa aaataaatat ctcatacttt
    21781 ttcatcttac actcctagaa atttttagct ttgaaacaat cctgtcgaaa catatacttc
    21841 acccactata atttttccac aattatcaat tattgtcaat ttatataagt atgtttatta
    21901 attgttataa ataaaaagct acttactaaa gcaaacagac gtttcacttt aataagtagc
    21961 tatattttta taacaaaata gttataatat tagaacttcc ttcatttaca attttttgta
    22021 aagtcttttg cattttaact cttatctctt ctggcatagt atataattta ctttgtaatt
    22081 gctcttttac taagtcatgt agtgacttac caaacatatt tgattcccat aaatctgctg
    22141 gttgctcttc aaatacttcc ataagatatt ttatcatctc ttcaccttga ttttgatttc
    22201 caactattgg agaaacctct gtagatatat ctgcttttat aatatgaagt gatggtgcat
    22261 tggcttttaa cttgatacca tattgttttc cttgtttcat tatttctggt tcttccagac
    22321 taagttcttc taaggaagga gctactactc catatccttt tatcttggca tctattaaag
    22381 cactttctat tttatcatac tcatttttaa ctttagatag tctagttatc agactaagca
    22441 gttgataatc tccttcaatt ttaaatccac ttttttcttc aaggacatta tagaataaat
    22501 cttgctttgt ctgtaaatca atatttataa caccttctcc tagctctaca ttatcaacac
    22561 ctgtatcttc caaaaactct agctcagaaa atccttgtat aataccttct atatccctta
    22621 ttttacctat atctataata ctttgtttta atgttgttat tatgctactt tttatccaat
    22681 gatttctttc aagaccttca acccattttg gtaagttgat tcttatttct gtaagtggaa
    22741 aatcatataa tacagcttcc ataacctcct ctatatcttc ttcttccatt tcaaccacat
    22801 tcattggaag aactggaact tcatactttt cttccaattc acttctaagc atactcgttt
    22861 cctcactttt tggacttaac gtatttaaaa ccactgcaaa tggtttttta agatttttca
    22921 attcttgtat aactctctct tctggttcta catagctttt tctgtcaatt ccagtaactg
    22981 aaccatctgt caagacaact atccctattg tagaatggtc ttttataact tttttagttc
    23041 ctatctctgc tgccttttca aatgtcatag cttcttgtga ccaaggagtg gatactaatc
    23101 tttgttttcc accttcctca tgccctaaag ctccttcaac tatgtatcca acacaatcta
    23161 ccattcttac ttttaaagat actgtgtctt ttatttttat ttctacacca tctgctggta
    23221 caaattttgg ttctactgtc attatcgttt tacctgaacc actttggggt atctcatctc
    23281 ttgttctatc ctttttaaat tcattgtcta tattaggtat aaccaacttt tccataaatt
    23341 ttcttataaa agttgatttt cctgttctta caggtccaac tactccaata tatatatccc
    23401 cctgagtcct tttggatata tcttcgtata tgttattatt catattattc ctcctataca
    23461 tctaatctta ttaaattata tttagagtta agggttaata ttcttaaaat agaaaaataa
    23521 agaccagcaa tattaattta atttacatat agaaatttgt aaatttcaat gtaaattaaa
    23581 ttaatattgc tggtccaaat agtagtgtat tatattaact gactttagta gtattttcat
    23641 ttgaattatt attcatctca ataagttctt tacgtctttc ttttgtcatg ttcatccata
    23701 taaatgtaaa caccaatcca attatggcta ctatagtaag aactatgaag tagtacctat
    23761 atccagttac acccggaaat gcatccaata tcttccctgc tgctaaagga caaagtactt
    23821 ctggaagata tcctaatgta gcaactattc ctatagcagt tcctgatatt gataatgaat
    23881 aattaccttc ttcaagtaac gaatagtgta aaccttgaat tgcatacata gatacatata
    23941 ttgcaacaca tccaatcaat agtatataaa tcatagatga ttttgttggt gtaaatatta
    24001 cacctaaaag acctgcaatc ataagtataa atccataaga tataatcttt gatgaaccaa
    24061 ctttatctcc aagtaatcca gaagcagctg atgctactgg tctacaatac tgagctaata
    24121 ttgagattgc agatgcaaac accacagtag ctccaaatga ttcagttgaa taaggcgtaa
    24181 aataataaaa tgacatatta gctgaatatg aacaaaacat tattattgtt attatccatg
    24241 tatgaggcat ttttattact gcaaaaaagg cttctttatc aaattttgct gatacctctt
    24301 tttcattatc ctttaatttg aaaaatacta atactccaac tattacattt attgcgagta
    24361 taatactata atagctgtaa gaccagattt atcacttact tttgatgaaa tatacccaaa
    24421 tagtatcaat actagagata gatgaattgc attggtaatt ccacgaccac tttccataaa
    24481 tccaaaagct tttccttgtt cattttcaga tgctaacatt ctaacagctt ttacaagagc
    24541 tggccaaaaa gtaagtattg ttgttattcc ccataaggca tggattaaaa acacaacaat
    24601 atatggagga taaagtgtta atacaagacc agatattcct gtaataatta aagaacctga
    24661 aagcaatttt ctggctgaaa tacggtctgc taaaaaacca ccaaaaaggt atgataccat
    24721 tgcaagtaga ccataaatac ttcctaagct tcccatttgt gtatttgtaa gattaaatgc
    24781 ttgtgcaaaa gtatcataat aataattctt taaatatggt aaagcatata taaatgctcc
    24841 tgctacaaag agtattatca agactttgaa atttttccaa atgcttggtt tatttacttc
    24901 aactgacata cttcctcctt atgtaaatac ctgtaaattt tcataataat taattattgc
    24961 ttattttatt gtttattcaa tatttaggaa tatttattaa tatattaggt taatctatca
    25021 ataatttatg gtgagttatg agctaaaagt aaactctttt agctcaccat aataattata
    25081 tttttattta tactaacatc cagagttatt agatatcttt ttttcatagt tcttccatag
    25141 cattaaacct gttattataa caaaaagacc tcctcctatc atttcaatct catagattat
    25201 attatctttt aaagtctcca taactggtgc cgttgagaaa agtatgccaa ctacagttac
    25261 tacaagtacc attctggcaa tattgataca aactgaatca ctttttgcaa ttgaatatgg
    25321 tctttcttca tttggtcttg tctttctaag tctaatataa gatgtaaaaa gcaagacata
    25381 tggaaataat gctgttaatg ctgtcatagt aacaagaaca ttgtatatat tatctactga
    25441 tggtaataag tttgttccta caagaagtag actcactatt atagcctgaa ctattactgc
    25501 atttgatggt atattgtatt tatttgtaac agtcaattgc ttaggaaata caccttctct
    25561 tacatttcca aaaagcatct ttactggacc tgctatataa agtattaatg ctcctagtac
    25621 tgataaagtt attccaaatg ctactattct tataaaccaa cttcctatac ctaaatcttg
    25681 tgcaacttta gctaatgcat ctagtatccc tgtagatgct gcaattttat ctggaggaag
    25741 tatcattgtt attgctactg aacctaagat atagattcct cctattaaaa cagccacagt
    25801 taatatagct ttaggaaagt ttttcttagc attatccatt tcggttatga aattagcagt
    25861 cgtttcagct cctgataatg caaatattat agcagatata gaaactaatg aattggcatc
    25921 tatttttggt attatatttt ggattgtata tatcgatgct gatggagctt ttttgagaat
    25981 aactacagac ataaatgcca tcactataag taatatagca ggaattgtag aacctaatgc
    26041 tcctgtattc gtaaaaaatt ttccaaatgc catcccacga gtacttacta aggacaacag
    26101 ccaaaatatt atgagtgaaa gtgctaaaat aaacatctta ttgtttgata aatctggatt
    26161 tccaagcata tatgcaatat ttatcgctaa aaaagtcaag aaagaagaat accaaaatat
    26221 ctttgatgtc caatttagcc atgacaccat aaatccccac ttctctccat aagcttgttt
    26281 aacccattct ccaagtccac catcttttgg atatgttgca gccaactctg cacatataag
    26341 tgatgcagga acaaagaata taaaagcaaa caataaccaa actggtatag aaccaagacc
    26401 caatccaaaa ctagaggcag tagatttagc tatccatcta atcccaaata gtgctgatat
    26461 attcataaat actaaatccc aaaatccaat tttctttttc actgtaattc ctccaatttc
    26521 aaatttattc agactcatac acaatgtatt gacttaaata tcaagtataa gttcttcaac
    26581 acttcttgga taattagtta taattgtagc accattctta cctactattg ctgtatctga
    26641 gtgtctaaat cctccaactc caggtatata tatccctggc tccatagaaa ataccattcc
    26701 ttcttctaat attaattcat tgtcaaatct taaatatggc tcttcatgtt ctgataaacc
    26761 taaaccatga ccaattctat gatttgaata taactctaga ccatactttg caacaacagc
    26821 tcttgctgct tcatccacca ttcttgctgg tataccagct tttattgtat ctaatccagc
    26881 ttgttgagcc tctactgcaa tcttaaacac ttctttttgt ctttctgttg gtttgcctat
    26941 aataaaagtt ctttcatttt ctgctctata gttttcatac caaacctgtc tactatgtat
    27001 tactatatct cctctttgaa gtattctagt attggaatct aaatgaggtt gtgctgttct
    27061 atcaatccct gaacaagtcc agtttgcaaa tcctatatat gtatcaggat aattttcaga
    27121 aacatatttt agtaaagcat tatcccctgc aacgtcaaac tccaattcac tcatcccaac
    27181 tctaacattt tctaaagacc cttttattcc tatatcagat aggtaacctg ctatcttcaa
    27241 aaattctatt tcttttgcat cttttacata tctcatttca aatattttac ttccaacatc
    27301 tttgatagca aatgacttat cagttatata ctctgaaaag cttgcagata ctatatcttt
    27361 ttctattcca acagttgttc ctttagggta tttatttaaa attatatcta agtaatgttt
    27421 atgtgatatt ccatgttctt tcatttctgg tatttcataa tacacatata cattatcaac
    27481 ttttgcaact tctttagcat gtaattcttc taatccaggt actattaatt ctattttatc
    27541 ttgagttact actgttacaa ttggtctaga ataagttatt gctttaaatc cactaatata
    27601 aaattgattg tctggttcaa atattaaagc aatttcaatg cttttttcat tcatgtactt
    27661 tctcaatctt tctagtctca tattatttga cataatgcac ctctttctta atattcagat
    27721 aatttattca atacaacaag gatgaatata ttattttaaa attcttaata tttttagtac
    27781 ttgtcgtatt tgtatgtaaa ttaattttat ttgtgattat tttatcataa tcatgattat
    27841 tttgtcaatc atgattttat tttaaaaaat tttttatatt ctagtgattt taaattatct
    27901 aaaatataac gagtatacat tgtattttgt aatattattc ttgatttatg gtaagtttaa
    27961 tgataaaatc acgattatat aaactatatt gattatttgt aggtgaataa aatgacaaat
    28021 aaagaattac aaaagaaaag gatattgatg tatttcatag aagctgctca aaatattatg
    28081 gaaaacgaag gtatagaaaa tattacactt cgtaaggttg cagatatggc tggctataat
    28141 agttcaactc tatataaata ctttaaaaat ttagaccatt taatatcatt tgcatctata
    28201 aaatatttta aagattataa cttgaatatt tcaagatgta tagaaaatgt caatgatgaa
    28261 tataagaaat atataattat gtggaaatta ttttgtaagc attcttttga taatgctcaa
    28321 gctttttacc aggtattttt taacttgtct agtgatgaac ttagctatat aacaaaacaa
    28381 tattatgata tgtttccaga agatttaggt attcatgata gtgacatatc tttaatgatt
    28441 acaggagaaa gtataaagga aagaaataag atactattaa acaatcttgt agatactgga
    28501 tatataccag ctaaagattt agacataatc aatgatataa ttatttcttt ttatcaaaat
    28561 ctattattct taaaaaagaa cacaggaaaa aattctgatg atgatgaatt aactatgaaa
    28621 atgatttctt ctattgaata cataataaaa catgctaaat aaaattaaaa tatggctgtc
    28681 tcaaaataga gataagcctt ttttattgtt gaaataataa aaatatattt aatttcatat
    28741 aaaaaagagt gcctcgtaaa ctaaaaagtt cattttgaga cacccctaaa ataaatttat
    28801 tatttattat acaatagttt atatatcatc acctaaaact acttcttcca tttcatgctt
    28861 tttacttctc atcattagtt ctattccaac ttctttagga tttgaacctt tctttattac
    28921 agaatatata gcattagtta taggcatatc tatatctaat ttttcagcaa catcatgtgc
    28981 tacttcagta gcagttatgc cttctacaac catctttact tcttttaaag tatcttctaa
    29041 actcatccct ttacctatga gtataccagc tcttctattt ctactatgca tacttgtaca
    29101 agtaactatt aaatctccaa tacctgaaag acctgcaaag gtagacatat tagctcccat
    29161 agcaattcca agtctgctca tttcactaat acctcttgtc ataagggcag cttttgcatt
    29221 atcaccatac cctaatccgt cacatatccc agctccaaat gctataatat tttttaatgc
    29281 tcctccaagt tctactccaa ctatgtctgg attcgtataa actctaagtt tagggctcat
    29341 aaataaatct tgtatcatct gtgctatttt taaatcttta gaagcaacta ctacggtagt
    29401 aggaatatcc cttgctactt cttcagcatg agatggccca gatagtatta catatggatt
    29461 ttgtggtaat tcttcttcac agacttgaga taatctaagc ccagtccctt tttcaagacc
    29521 tttagctaca ttaacaataa cttgtccttc ttttataaat ggttttattt tttggcaagt
    29581 acttcttaca gcctgtgatg gaactgctaa aactattacc ttacaatctt ttatagtttt
    29641 ttctatatca gtacttatag ttatattatt tggaaataat actcctggta aatagtctgt
    29701 attttcttta gttctgttta tttttttagc ttgttcctct tttcgagtcc acatgctcac
    29761 atcattacct ttttttgcaa gccctaaagc taatgcactt ccccagcttc cagtacctaa
    29821 tacacatact ttctccatgt ttaacaccac ctgagtataa tatatttact attctttctt
    29881 ttgaccaatt tttctctctg tcccatttaa taatcgcttt atatttgctc tgtgattata
    29941 tattactgat gcagttaaga acagagctac tattaaacca gctttatttt ttaccataat
    30001 cataaatatt ggtgaacatc ctattcctac aacagaaccc aatgaaacat actttgttat
    30061 agctaccacc aatataaata ctgctagaca cattagtgtt attactggat ttacagctag
    30121 cattgcccct agtgaagttg caacaccttt gcctcctcta aatcctaaaa ccgctggcca
    30181 attatgacct gctacaacac atataactgc cagatatcca gctaaaagag tatctattcc
    30241 aaccaatctt gctgcaaatt ctgatattaa aacagccatt acacctttaa gaacatcacc
    30301 taaaaatgtc attgctcctg ctctttttcc aagtgtccta agtacatttg tcgaacctgc
    30361 attaccagaa ccttgagtcc ttatatcaac cccagcaatt cgctttgcaa ctatgtatga
    30421 ggtagatata tttcctaaaa gatatgcaac tacagcaata attatataac taaatatctc
    30481 cactgaattt ccccccttta gttatatttt aagatattta ttttttattt ttttgtctat
    30541 actcaaactt tatggaagtt ccttcaaatc caaaattttc tcttatctta ttctcaaggt
    30601 atctttgata tgagaagtga gttaaatcct tatcatttat aaacaatgta attttaggag
    30661 gtctaatgtc agtttgagtc ccatagtaaa tttttaatct tctacctttg tcagatggtg
    30721 gttgatttaa cattacagct tctccaatta cttcattcag tgctgaagtt gatattctct
    30781 tagaatgttc atttgacaca tattcaactg tatctagtat cttgttcatt ctttgattag
    30841 tttttgcaga aacaaatacg attggagcat acatcataaa tggaaatttt tcttttatat
    30901 cttttgtgta attgctcata gtcttattat ctttctctat taaatcccat ttattgatta
    30961 caaatatgca tccttttcct tcatcatgtg ctatacctgc aacctttgta tcttgttctg
    31021 ttacaccttc taaggcatct attactatca atactacatc tgctctatct acagcactca
    31081 tagcccttat tacactgtat ttttctattg tctcatatat tttacttttt cttctaagcc
    31141 ctgctgtatc tattagaaga aatttctgtc catttttttc aaaataagta tcaattgcgt
    31201 ctctagtagt tcctgcaatt ggacttacaa ttactctttc ttctccaagt atcttattga
    31261 gtatagaact ttttcctgca tttggtttac ctgttatagc tactcttatt atatcttctt
    31321 catactctgt atctaatcct gctggaaaat tttcaactat ctcatctaat aaatctccaa
    31381 atcccatact attggctcct gaaactgcaa aaggcgttcc aaatcccaac tcataaaaat
    31441 cgtatatatt atcaaattga ctttgactat caatcttatt aaccactaag attactggtt
    31501 tttttgtctt tctaagcaat tgtgcaattt ctttatcagc agctgttatt ccagctttac
    31561 catcaactat aaaaagtatt acatgtgaca tgtccattgc aagcatggct tgatttctca
    31621 tttgagataa tattatatct tcgctatcag gttcaattcc tcctgtatcc actaatgtaa
    31681 aatatttatc taaccattct acttcagcaa atattctatc tcttgtaact ccaggtgtat
    31741 tttcaactat tgatatcctt tttcctgcaa atttattaaa tattgtagac ttaccaacgt
    31801 ttggtctccc aactacagct acaactggcc tacttatact cattttattt ctccttccat
    31861 cttttgtttt ctattttaaa attttatcca caaaatcttt tccttcattc aaacaaggga
    31921 taatttttat tctcataagt tcttctaatt gatttaattc aatattatct aagaaaattt
    31981 cttcatctgc ttttagcata cttcttgtta tataaagagc ttctcctaat tctttatctt
    32041 caagttgttc ttttaaatcc ttagctgtta aaagacctga tacagttatt gtctctccaa
    32101 aaaacttgtt tttgatttca tatacattta cttgaagatt tttaaacttg tccatcatag
    32161 catctgccat actctgtata aattcatatg cagaatgacc agtagctatt gatactttct
    32221 tctctctttt tagaatcttc tcaggcaata tatttagata ctcttcaatc tctgttttta
    32281 acttacgtat catacctaca ccatcttcaa actgtaaaaa accttcatat tcttcatatc
    32341 caggcaattc actattagat agtatataaa attcatctga taaaaatgca aatcgagtac
    32401 ctaatttttc aagatatttc tgttgtattt gatgtatttg ctctatagtt ttacttgcgc
    32461 tcttatcatt aaatatattt aattctacta gattttctct atgctttgta atcccaactg
    32521 gaactatcgc aacactgttt acatacggat aaagttgagc tagttctttt attgttctat
    32581 ccaattcctt tccatcattt atacctggac ataaaacaat ttgacagttc atttctatat
    32641 gagcatctgc caatctcttc attataccat ataattttcc tgcaaattta ttgcttatca
    32701 ttttttgtct taactctgga ttagttgtat gcacagatat atttatagga cttattctat
    32761 attttataat attatttata tcttcttcac tcatatttgt aagtgtgaca aaatttcctt
    32821 gtaaaaaaga taatctcgaa tcatcatctt taaaataaag agtttctctc ataccttctg
    32881 gcaattggtc tataaaacaa aacatacact tatttctaca gcttttagct tggtcaatta
    32941 tcggatttgt aaattcaaca cccaaatctt catcatagtc tttttctatc tcgtatatgt
    33001 agacttcgcc atcttttttt tgtatttcta catctaaata ttcatcactt agtaagaatc
    33061 tatactctat tatatcatgt attggctgtt cattcacaga tataagtaaa tctcctacct
    33121 ctatacccat ttcttctgca atactatctt tataaacctt acttataata ttatttactt
    33181 tttttacatt tacttccaat attttcacca ctcttttgta ttcatcaatg cttattataa
    33241 tgtattttaa tatgcaattt atttatttat tatattttat gatagcattt ttaagcattt
    33301 catgtcataa taacacattt taattctaat agtcaatata agtaaatagc tataaaatag
    33361 attagaacaa ataatattca aatttaagtt tcttaaaaat ttaaatatta tttgttctta
    33421 aaaaaatttt ataaatttaa tgttttacta tgattaacgt cccatttgtc atatcatgaa
    33481 ccattccacc tacacacttt gacatcaaca atgccttatt ttgtaagtct ttctcatcat
    33541 ttgcataaaa tattggacaa cctccaccat ttttcatatt cttatcaagt gtaactatag
    33601 ctagggtata ttcatttaaa cctatatcca ttcccatctc atgcacttcc tttctttcct
    33661 attttataat tttttagtga taagtttttt cctttagagc ttgaaagtat tgggcatgac
    33721 tttacagctt ccattacttt ttccacattc ttatctactg gtaaataagc aatcattata
    33781 ctttcatcct ctttatttct tctaggtaat ggtacgaatg aaggttctcc ataatctcca
    33841 tataaaccta accttgaata tatattgtat actattgctt gtctttgacc ggagtcaaat
    33901 aatattcctg cattagcata atccttattt ttaggaatta tttgtattcc tacacctcta
    33961 ttaatatata cttctctatc gctttttaga cctatatttg ttataccttt taaatctccc
    34021 acctttagta tagattcgtc cacaaaacta atttcagcag gcattacatc agctatgtct
    34081 ccaatacttt ttctagtaag aagttttttt aatataaagg ctaacactaa tccacaaatt
    34141 gaacttatta ccaaactcat cccttcactt attttcgttt ctgcaagtat tacataatac
    34201 aatcctattg ttaaaaaaga tgtaactata cacatataat ttcttacttc ataggttcta
    34261 gctatctctt ctatgaatgc agctcctctc tttacaagtt gaacttcctc taaattttgc
    34321 aatgtatttc ttctatttgc tctcacttgt ctaaattgtt ctgctgctag agataaaaaa
    34381 gttatagatg tataagaacg atttataaga gctggaacca ataatgctcc taatgaagaa
    34441 gcgacgaatg ctaatataag ttgagatagt aaaacatttg gttgcgttgg atactgtttt
    34501 tggtctaaat tcaacattat aattctagat gttataccaa taataacagc aacaaaaaat
    34561 actctatcca ttaaatcact cctaaaatat ttaatttgaa attttacatg taaatctaca
    34621 aagtctttct agaaaaatca ctctgctttc cttttgatgt ttctatgata ggtgtacttt
    34681 tcactacatc tatcatgaca tctatatctt tgagtattgg catatatggt ataaccacag
    34741 tacttttttc taaattggtc ttagatatag caacaatatc aacctcatct acatctctat
    34801 ctatccccat atgaataaat agattatgta ttatagcttg tctttgccct atatcattta
    34861 ctattccaaa tgcacctaaa tctcttggta taacttctat ggccagacat tcatttctat
    34921 acttttctct tgtactttct agacctatgt tagttattat tacaccattt accattaaaa
    34981 taggtccttc aaaacttatt tttgcaggaa ctatatctgc tatatctgct atactgttcc
    35041 ttcttaaaaa tcttctaaaa atcaaaccaa caatagctcc tgacacaata gctaggattg
    35101 tgcaataaaa aaagctaagg ccatactttc ttgcaaaaac gatataaact atcgaagata
    35161 ccagtgcaga aaaaagactt atataacttc tagattcata cgtagatgct atttcttcaa
    35221 cgtaagcgtc tccttttggt actaattctt cattatcgat attctttagt gttattcttt
    35281 cttgttctgc aagaccttga aactgttgta ttgctactgc aaaaaatgtc aaggctgcaa
    35341 actccttatc tataagtgca ggtaatgcta tagcacctaa cgaagctgaa agcccagata
    35401 taattatctg ttctaaataa tcttgtggtc ttgatgggta ttgtttgtct gtaactctta
    35461 aaactaatcc tctacataat attcctacaa ctaatgcaac tataaaagag tgtctaaata
    35521 aatttgcatt caataaactt tcttccattt tctcagctcc ttctcttata tagtttgtgc
    35581 aaaaaacaaa aaaaattccc ttctatttaa ggaaattttt ttgtaatatt gtaactataa
    35641 attcgctacc aatgccaagt tcacttttta cttttataca tccatttagt gatttgacta
    35701 tatgctttgt tatagccaat cctaaacccg ttcctcctac atccctactt cttgctttat
    35761 ctaccctata aaatcgttca aatatgcgtt ctacatcttc ttttggtatc cctacaccat
    35821 tgtctataac tttaattacg attttttctt catcttttaa cactacaact tgtacaatcc
    35881 cattatcttg ggtatacttt atagcattat caactaggtt taagaacact tgctttatat
    35941 agtctttatt ggatagtata cttattgatt tatcatcaca tttatataaa acttctatat
    36001 ttttttcact agctgaatgt tgagttattt catatacctc tttaaataca tcatatacta
    36061 caacattttc cataagaaga gtatctttat tttctacaaa tgataacagc aatatatcat
    36121 caatcaacct ttttaatcta tctgattcgc tttctattat ttctaagaat ctatttctag
    36181 tattcacatc tatattttca tttgacttta gtgtttcaac aaacccactt atagatgtaa
    36241 gtggtgtttt aagttcatga cttacatttg caacaaaatc acttctcata ttttcaagtt
    36301 taactttttt agtaatatct tctatattta ctatcgaacc tattattacg ttgttagtat
    36361 tctgaagata aactgggtct acttttatct tatatacgat atcatcttta gtagtaatat
    36421 tagcagtttc attatcttta gaaccaataa attgaagtat ttgttttaat atttcttctt
    36481 cttttattac aacattaata ttatgacctt caactaattg atttggttca ctctttatca
    36541 tttcttttgc ttcatcattt atcagaagta tatttccatt tacatctata gctaaaattc
    36601 catgagatat actctttaat atagacctta actgtaaatg tttatactgt acttcttcta
    36661 aggttttatc catattcttt atcatatgat taaaatttct agctaattcg ccaagttctc
    36721 ctttggctga tatgttaagc ctagcatgat attcattatt acttactttt tttgagacgt
    36781 atataaactc ttttaaatat tttcttaatg ttattgtgta tcttacagct aatataacaa
    36841 tggctatgga caacataaca ataaaaaaat atatactatt taaatcttcc acaagataac
    36901 actcctaaaa tataaccttt ttaatctatt ttgtatccta cacctcttat agtctctata
    36961 tatttttccg atttatcttc atcttctatt ttttttctta aatatcttat atgaacatct
    37021 actgttctag tttctccata gtattcatac ccccatattt tatccaaaag ataatttctt
    37081 gaaagtactt ttcctttatt ttgaattaat aattttaaca attcaaattc cttaagagtt
    37141 aactctatct tttcagagcc tttggtaact tcatgttttg ataaatctag ttttaaatta
    37201 cctgtagtaa gtatattgtt ttcttcacct aagctagtca aattatatct ccttaaaaca
    37261 gaactaatcc tagctaacaa ctcttttata ctaaatggct tagttatata gtcatcagca
    37321 cctatttcaa gcccttcaac cttgtcattt tccatatttt tagcagtaag cattataaca
    37381 ggaatatttt ttaagtcctt atcacttctt atttttctca aaacttctat tccacttata
    37441 tttggtaaca tccaatctaa aagtattaaa tctggtttaa tttccttagc ctttataaat
    37501 ccgtcaaatc catcatatga ataactaact tcgtaatttg acacctccaa attgaatttt
    37561 aatagctcaa ctatatgcat ttcatcgtct ataacaagca ctttagtatt cattaaaaag
    37621 ctcctctcta ttttatctgt agtaccgttc ctattcgttc tgatgcctta ttatgttttc
    37681 tataagagtg gaatttatct gctctacaac tagtgcataa atttaaattt attatatttt
    37741 cttttttaac tccactacat ctaagcatat actcattaat tttccataaa tctaaatagt
    37801 aacttccctc ttttattata tagaactttt catcattatt tgtaagaatt gtgttaaatt
    37861 tttctactaa atcttcagac acttcataac aacatggtcc aattgatggt ccaattatac
    37921 atactaaatc ttcaggaact gttttatagc attcagacat taattttatt gttttatgac
    37981 ctatattact aaaagtccct ctccaacctg catgagccag tcctatggct ttatttttag
    38041 catctataat tgatataggt acacagtctg ctgtaaacag taaaagtggt actttttcca
    38101 agtttgttat aagtgcatct ccatctcttc ttttccctat agtatcttta ttgactatag
    38161 taactatatc tgaatgtatt tgagaattac tagttagatt ttcaaatgtc aaatcacatt
    38221 ctttacatac ttttaacata tcttctctat tttttgcatc tatattctta catgttataa
    38281 ctagatttac agaatttaat ttttcatcaa gttcttcaca attaccactt aaattaacat
    38341 actctttaat atcttgtaca ttttttatat ttatataatc tttcatcatt tgtctccttt
    38401 ttctatcagt atacttttaa gttcatttac aaaagtattt atatctttaa attgtctata
    38461 cacagaagca aatcttacat aggcaacctc atctaactct ttcaatttat ccattaccat
    38521 ctcaccgatt tttttggttt caatttcttc tataaaacat ttatttattt cattttcaat
    38581 atgtacaaca atactttcaa tctcgtcaat agaaacaggt cttttttcac atgcttttag
    38641 caatccatat tttatctttt ctctatcaaa atattctcta ctattatcct ttttaatgac
    38701 cattaaagga gttgtttcta ttttctcata cgtagtaaac ctctttttac atgattcaca
    38761 ctcacgtctt cttctgatag attttaaatc tgtatgtcta gaatctatta cctttgattc
    38821 tttataatta caataaggac attgcatcta tactcctcct ttgtttaact atatctcaaa
    38881 ctcatctaag cttaattctg aacctatatt tactattatt gtatcacaac ctattttaag
    38941 tatatcattc caaaatataa cttcttcatc ccctcctctt gaaaaaaaac ttctacttcc
    39001 atctccaaaa atggtaaatg agactacctt accttcgtga atatccatgt ctatgtctat
    39061 tataaacccc atcctttttc cattttttac atttataatt tctttttcca ttatatcaga
    39121 aactctaatc atattgtact caccctttct ttaaaaataa atatgtatcg aagtaaaaaa
    39181 aaatgaccta tataggtcat ttaaattaaa ataatgttta cagtccatgg attacttata
    39241 catattttct catatttttt aaagcatttt tttcaattct tgatacttgt gcttgagata
    39301 taccgatttc atcagcaact tctatctgag ttcttccttt gtaaaacctt aaatctaata
    39361 ccaacttttc tctactattt aatttcttaa tagcttcttt aagtgatatt tcttgcaacc
    39421 aattttcatc agtatctttt ttatcttgaa cctgatccat aacgaatatt gcatctccat
    39481 tgtcttgata tactgggtca aacagagata ttgggtcttg tatggcatca agtgccatta
    39541 caactgattc tacttctaat tctaattcct ttgctatttc tgatactgta ggttctttag
    39601 aatttgttct aattaatctt tctctaactt gtagtgcctt atatgctata tcttttaatg
    39661 acctacttac tctaattggg ttattgtctc ttagatacct tctaatttct cctataatca
    39721 taggaacagc ataagttgaa aaccttacat tttggcttaa atcaaagtta tctatagctt
    39781 ttataagacc aatacaacct atttgaaata aatcgtctat attttctcct ctgttgttaa
    39841 acttttttat tacactcaag actaatctta aattccctct tacaaactgt tgtctggctt
    39901 cttcatcacc attttttatt tgtaataaaa gttccttcat ttgcttattt ttaagaactg
    39961 gaagttctga tgtattaaca ccacatattt caaccttatt aacttgcata tattttcagt
    40021 cctctctcaa aagtattcat ctattgaaat tatttacatt tccaattttt ttatactttt
    40081 taagaagaat taatttagca ttatacaaat tttttcattt ctttttgcaa ccttgagatt
    40141 attttctttt caagtctaga tatgtaggac tgtgatattc caagtattcc agcaacttct
    40201 ttttgtgttt tttcaatacc tttatcaata agaccaaatc ttaattccat aatttgcttt
    40261 tctctgtcac ttaatctgtc aagtgccata accaacaaat ctctatctat ttcttcttca
    40321 attattttgt atatttcatc attttctgta cccaatacat cagataataa aagttcgttt
    40381 ccatctaaat ctatatttaa aggttcatca aatgatactt ctgttttctt tttattattt
    40441 tttcttaagt acattaatat ttcattttct atacatctcg aagcataagt tgccaatttt
    40501 atatttttat tcagtttaaa agtattaact gcttttatca aacctatagt tcctattgaa
    40561 atcaaatctt ctacatctat acctgtattt tcaaattttc tcgatatgta gactactaat
    40621 ctaagatttc tttctattaa aattgacttt acactttcat ctgtttctag cttttgtaaa
    40681 agctccattt cttcttctgg tttcaatgga ggaggaagta tatttgcccc tcccatatag
    40741 tatattccct ttggctttat tatttttaat ttaataccta acatagtaat aaagcttatt
    40801 attctttctt tcaatcgtaa cattccttgt cccccttaaa ttttcaccta aaatccaggc
    40861 aatatatttg gattcattat tgctccatat tctctatcat taaaatttga aataccaagt
    40921 attatatttc ctatcttttg ctcatctact tctatatagt cagcttttaa gcctattatt
    40981 atgcttgtct tattacttcc agcatgttta taaggtatta ttctaactct acctgaagtt
    41041 ttatcatcta aacttcctat aatttcttgt gctttcttca tatctatatc ttcataatta
    41101 atatttaata gtttttctgg aataaatcct tctaacacac tagacttaac tattattaca
    41161 tcactttgac ttattgggtc ttttaacaaa ttaccactat ctactaaagc cttacagcaa
    41221 aaactttttt ctagtaagtt tatatttata gtctttgtaa gctccttaat gtacttaagc
    41281 attttcaaat ctttataaat gtattttaat aattcacaac tcacataagt acatgcaatt
    41341 aagaaagata tttttaagtg acttattcct gtaaaataaa ttataaaata agtgcttcca
    41401 gaaataaaaa tgtttactag ataaaaaact aacgctacat gaacataatc ttttttgctc
    41461 ttacttctaa atgaaattaa agttattata ctcatgatta ttattttaaa tggtacagta
    41521 aatagtatat tcattgatgg atacaaataa gcaacagaat aggcacctcc taaactagcc
    41581 cccaataact tatttttttt aggattatat cttttagtaa gaatagaagt acaacttata
    41641 ataatataat ttattactag gttttctata acatagtact caatatacac catgtatccc
    41701 cccttaaaac ctttaaacat attataagat agtatgtaca aaattttatg tcacttttga
    41761 tggtcgaatc caaaacaaat tctatttaaa aagaacaatg atatatgtct tatactttat
    41821 gtcgtcaaaa gatatttttt taatgccaaa caaatagact tttttgtaga atccaatata
    41881 tctaggtaga agttattcgc tctacttttg gtatatttac aaaaaaagac tacataattt
    41941 aataatatat caagtaaatc ttttacttat ataaaatcaa attatatagt ctgtaaattc
    42001 aaatataaat tattgtgcta gtaatccatt tgcatatttt gcagattcat ttagtgcttg
    42061 ttctggagtt atgtctccag ctatcatcat ttgaagttgt ttccataaat attcatatat
    42121 ctctactcca tgaggtccaa ctgtcaatgg tctatacata tgtttatctc ttgtcaccat
    42181 ttcttcaaat cttgggtctc cttggtattt ttcaccttta gcaactggag ctctaggtat
    42241 ttctttgtgg aaagcttcca ttgattcttt gcttagcata tactcaagaa gtttaaatcc
    42301 aagttcttta tttttacatg ctgacattaa tgttaaatca tctactgaag caaaacttcc
    42361 aactgtctta tcttctaatg atgttacata tccccagttt aaatccggga atgatttatc
    42421 aaaagtactt gtagcagcag aggatagcat tatagtaaat gcagcttttc ctggtccaaa
    42481 tacactttca atcatttcat tactatcttt tgataaagca tcttctggaa tatacttttt
    42541 catatcattt ataaagtttg cagctttcaa gccttctgca ttattaaatt taactgattt
    42601 taaatcatca ttatatatat ctccaccagc ttgccataaa tacggatacc agttccagtt
    42661 taagcttcca aatactttag aaccccaacc ttgtgcaaga ccccactggt cgatttttcc
    42721 atctccatca gtatcttttg ttgctttttc acagattctt ttaaaatcat cccatgtttt
    42781 tggaggtttt tctcctaatt tttctagtat atctttattg tagtacaata cgcctggatt
    42841 agcagcttct ataggtaaac cataaatacc acccatcatt tttgcatcat ctaagtatag
    42901 ataattatct gaagttcctg atttttctaa ataaggtgtt aaatcttcta ccgcacccat
    42961 ttctataaat tgaggaaaca tctctgcata catgtatcct atatctggac cttcacctgc
    43021 acttattgct gttgcatatt tttctgcgta gttatcccat ggtactatct gaaattcaat
    43081 ttcacagtta ttttcttttt caaatttttc aaatatcggc ttccatgcat ctgcatcatg
    43141 gtctgttaat ggtgcagacc atacaactaa tttttcttta ccctctttgc ctttagaatc
    43201 ttcttttttt gaacatcctg caagaattga tactgataga atccctacca taactaaaga
    43261 aagaaccttt tttagtttaa tcatgcttaa tcccctttca gactttaatt tgtctttttc
    43321 ctcaaatgtt tacatttaat tcagatttaa tattaacaaa taagattact cttgtctaat
    43381 cataaaaaac tatactaact cctataacta tatttttttt ctatacctat tagatttaat
    43441 tcatactaaa aacttacttt ttaaatttta tcaatattta aaaatatttt taagtctagc
    43501 cacaaactaa ctatctaaat tgaacaaaat aaaaagaagt ctattgactt ctttataagc
    43561 ttacatatta tcttcttctt cttaggaatg taggtatctc catgctctca tcatcatctt
    43621 cttcaattat acttgccttt ttaggaggct ctattttttc ctcagcaatt acttcttctt
    43681 caatttcttt tacagcttgt tttactgttg tatttaatga agaacgtatg cttgatttag
    43741 gtttagtatc taaatctaag gcaccatttt gtaatccttc aaatcctgtc gcgattactg
    43801 taatcattat ttcatcacct aaatcttctt ttatagaagc tccaaatata acatttgctt
    43861 ctgggtcaca ggattccata actaaagttg atgcctcatt aacttcaaat aatcctaagt
    43921 taggaccacc tgtaacatta agaagtactc ctttagctcc ttgtattgat gtttctagaa
    43981 gtggagattg aatagcttgt cttgcagcat caattgctct agtttcacca ctagcactac
    44041 ctatacccat atgagcaagt cccttgtctt tcattatagt agtaacatct gcaaagtcca
    44101 agtttattaa cccttcaact gctataaggt ctgaaattga ttgtatacct tgttttaata
    44161 catcatctgc aactgcaaaa gcatctaaca ttgatgtatt tttttgtact atctgtaaaa
    44221 gtctatcatt tggtatcgtt ataagagtat ctacttttga tttaagttct gctattccac
    44281 cttcagcgtt cttcattctt atcttacctt caaatgcaaa aggtttggta actacaccta
    44341 ctgtaagtat ccccatttct tttgccaagc ctgcaaccac tggagcagct cctgtaccag
    44401 taccaccgcc cattccggct gttacaaaga ccatatcagc tccttgaagt aactttacta
    44461 tttcatcttt actctcctca gcagctcttt tccctacttc tggatttgct cctgctccaa
    44521 gtcctctagt taatttttct cctatttgaa ctttatattc agcttttgat gtatataaag
    44581 cttgtttatc tgtatttact gatataaatt caacgccttt tagttgagct tctaccattc
    44641 tattgacagc attgtttcca ccgccaccta cacctattac tttaatttgt gcacattctt
    44701 ctaattctac gtcaaagttt agcatcataa acccccttct ctcatgattc aaagttatcg
    44761 tatgtattaa ttatatcatt ttcttctttt attttgttaa cctttaagta acatattcca
    44821 caaaaacgta aattttcctt tttaaatttc ttacttatat tgtcaaatat tactctttaa
    44881 gtataaaata atttacgtat atattaaatg ctctttaatt tttacgtttt ttgttcataa
    44941 tattactaat aatatatcgt ctaattatag ataagttatt aaacaatctc ataccaaaaa
    45001 ctaatacagc aacatagtaa agagaaactc ctaacttttc tcctatataa gtcaataata
    45061 tagccaatat agcattacct atgaaaccag taataaaaat tacattatca tagttatctt
    45121 caaaattgga ttttatcgct ccaaaaatag aatccattat tgcaagaata gctactgaca
    45181 tatatatgga ataatccata gaatatgtaa atggaatata gtaacctact actacaccaa
    45241 atgctaatcc tcctaaaacc cataacataa atcttcacct ctttatttta ctttgaaaca
    45301 caactacatt acaatccttt ttattaattt tttttacttt ttctttattt ttttgtattt
    45361 ttcgttttat tttgcaaata aattcacttt atttccatta ttttcctgaa cttttatatt
    45421 aattccatat atatccttaa gagatttagc atatgattct ggagagttta tagctgcact
    45481 caataaatct atatctccta tagcttttat tataaatggt tgtccatatg tagtatcatt
    45541 tactgtaata gtagctcctg aacattttat cttacttgtt gctagtactc tttctccatt
    45601 tatagaaatt gctcttgctc cagccttttt taaatcattt aaaaccctca aaatatctat
    45661 atcatgtata atcaaatcgt ttggattctg tccgtctttt aactctctct cactatccct
    45721 catagtaata gttatgcctg gccctgtaac agttgaataa ccacttaact ctttaacttc
    45781 ttcaagctct ttttgcataa gtgtttggat agattcattt ccattctttt ttgcttcttt
    45841 atattcattc aaagtttttt tgttcttttt aagagtttct tttaaatttt ttatttcttt
    45901 ttttgtattt tctacctcac tttcaatact ttcagtttga cctaatgtta tgtatactct
    45961 atttgggtct agtgatttta tataagttga tatgaaaacg cctaatatga atgctcctaa
    46021 tataactatt ttctttttca tactattatt tcccctctgc tttgtttata tcactatgac
    46081 catttatttc tatattgtct tctttggtca cttttacttt ggcattataa tatgtttcta
    46141 tttcccagac aataccatac tttatattaa gtgccgaagc caatgtatca gaatcaccaa
    46201 ttgcttttat tataattggt tcttttatga cagtgtcatt catataaagc ttgtcttctt
    46261 ttaaatacat atatgtatta gaaactattc tctggtcgtt tatagatatg gcacttgctt
    46321 gtgctgaatt taatttattt atcattgaca ataataaatc ataattatat agtatactat
    46381 catttttatc agagtttcct aagtcttttg tagaatttat ctcaattttt atgcctgttc
    46441 cttcaacatc tgtatatcca gctaatgttt catatttttc tatttctgac ttaagtatgt
    46501 cttgttctcc ttcattgttt ttgcctctat aagtctctat atctttcttc acactattaa
    46561 cttctgattg tagattctct ttttcttttt ttaaagattt aagttcttct gtaagttgct
    46621 ctcctctttt aaaagttgtc atccctctat tttctatatt aataatttta agttgtaaac
    46681 taacaagaac acccaataca atacttccta tgattatact tttatttata aatctttttt
    46741 tcaatatatc acttccttac aattgtctat ccttctggac tatacactgc caactttcca
    46801 tagttaagat ttatagtccc tccattagta tttttatttt gcaggtcaat cagaatttta
    46861 cttaccctag atatattata atctaagtta tcatcattag aaagtaagat ttttatatta
    46921 cttcttgtaa gcatttctat attgctttct ttttttaagt ttacataatt tatttttcta
    46981 taaattcctt cttcttttaa ataatttaaa gttttaaata cattctcctt agttttataa
    47041 tttttaaatt ttatagattt attatcatca atgctataat ctacatctac tatcaagtct
    47101 tttttacttt cattagaacc tcttagttct tccaataaat ttcctttttt atctatataa
    47161 caataattat cttcatcttt taatactgca aatatttctt tttcttttaa acttataatt
    47221 atcttattgg gaagttttct ttttatttcc acattttcta tatatggatt ttttataagt
    47281 ttatttttca tatctttaaa attgtatgca aaaatatttt catttagatt tacatttagt
    47341 tctttcatta tatttgattt ggtcaccctc ttatttccaa ttacatctat tttctttaca
    47401 tcaaataaat cactttttac aattatacat atacaaaaag ataacataag tataaaaact
    47461 aaaactatca tgacattatt cgtatttaac tttcttcttt ttttcaaata taatcaaccc
    47521 ctttggttaa taaatacacc tttagagctt gtttttccta caaaaaaatt ataatattta
    47581 aagtcaaatt tttgcatttt attacataac atccaaataa ggtattaaat tttataaata
    47641 aatcaatatt tcatcaaaat aaacatagca tactacatac tatttatata tttaaatctg
    47701 tatacataca aataaacccc taatttataa taatttagaa aatctaatat attataaatt
    47761 aggggtaaat atttacttat cttttcttta ttcctattac cttagcttga ccttctatac
    47821 ttggagtcgt ttcttttttt atttctttta cttccttaat aactttttct ttttttgttt
    47881 ttttagaagc tgatttttgt gttgaattgt atactttcat tatctcatca tatataaggt
    47941 ctatggcttc aggcttacct atagttttac ttgcatttgc catatcaacc aaaagttctc
    48001 tatctcctaa aagtttaaat actgctgtat ttaggctctc tggagttaga tttttttcta
    48061 aaatagcaat tcctgcccct tgtttttcta tactcttggc attatactct tgatgatttt
    48121 cagcagtata agctttaggt attattatag atggctttcc aagagccgtt atctcagcta
    48181 atgaaattgc tcctgcgcta cctataacca aatcacttgc tgcaagtgca tttgccatat
    48241 cttctaagta aggcacaact ttttggtatg gtttaagatt tatatcgcta atacttccca
    48301 taaattcatc ataatagctt ttgcctgtag caaatataaa agctatatct tcatttacca
    48361 tattctttat tacaagtctc attgcatcat ttatttttct tgaacctcca ctaccaccat
    48421 agcaaagtac cattcttttt tcgtcactta ttgatagatt ctttcttgca atattttttc
    48481 ttgataataa aatttctttt ctaactggat ttcctgtaaa aactaattta tcttctgctg
    48541 cctctggaaa tcttttatgc gaatcttcaa aacttgtaag tacctttgta actgttttag
    48601 ataaaatctt attagttaca ccaggaaaag aattttgctc atgtattatg gctggtattt
    48661 tccccataga tgcattaaat aaaacaggtc cactaacata tccaccagtt cctataacta
    48721 tatctggttt aaatttctta acaatctttc ttgattgctc caaaccttta aaaagcttaa
    48781 aaactctttt tacattatcg aaatctattt ttcgcttaaa accttgaact gttacagtct
    48841 ttaattcaaa tccatactta ggaactattt ctgactctat tcccttttcc gtcccaacaa
    48901 aaattatctc agcgtctgga tgctcatctc ttattttgtt tgcaatagct atagctggat
    48961 acacgtgccc tccagttcca ccgcctgata ataaaacttt catcaaatca tcatctcctg
    49021 ttaattaatt ttgacatgct ttgagatgtt taacactatt cctacagctc ccataaagat
    49081 ggtgagtgaa gttcctccat aacttataaa tggtagtgct acacctgtag ctggcattga
    49141 tgatgtcgca actgctatat ttaaagctgc ttgtattcca atttgtgcac ctatacctat
    49201 aacaaccata caagcaaata cattagaagt cttcaaagct attcttacac atctgtagac
    49261 taaaactaca aatagcataa taactattat acaaccaatc aatcctaact cttctcctat
    49321 tatagcaaat ataaagtcat tttgtggctc tggtatataa aaatactttt gtttactctt
    49381 tcctaagcct agaccaaaca atccacctga acctagtgca tatagacctt gtattacctg
    49441 atacccctta ccaagagggt cttgaaaagg gtctaaaaat gatgtaacac gactaagtct
    49501 atagggttca gcaattacta atgcagcaaa taaagcagcc ccactcccta tcatagcaaa
    49561 tacaattttc atatccattc cagcaacaaa tatcattaca aaagtaacta gaattacagt
    49621 acctgctgtt gacaaatttg gctgaagtat tatcaacgca aaaaatatcc ctggaactac
    49681 gagaagtggt acaacacctt ttgtcaaaga ttttatttta tcataatttt tttcaattaa
    49741 ttttgcagtt aatattattg tagcaaactt tgcgatttct gcaggttgaa atgttaaagc
    49801 tcctattccc aaccatcttt ttgctccgtt tgcttctatc cctaaagggg tcaaaactaa
    49861 caataataat actactgcta tagctccaat tgctgtagca tttttcttcc aaaaactata
    49921 atctattctt gatgtaataa tcataactat aaatccaagt acagcatata ttacattctt
    49981 ttttagaaaa tagtacgcat cattgtgttt aaatgaagat tgtataaaac ttgcactaaa
    50041 caccataact attcctacaa atactaaaag catagtagtg taaaacacta ctccatcaaa
    50101 ttcagtcttc atccttatgt caatttgttt tttcaaattt tctttaggca tccaactaat
    50161 ctacctccat tcttccattt aagtaagttt agaggtaaga gattatttta aattgtttac
    50221 attatctttg aagtcttttc ctcttacttc aaaactttca tacatatccc aacttgcaca
    50281 agctggtgaa agaagcacta tatctccact ttttgaaatt tcataagaag tcttaactgc
    50341 ttcttccata tcttttacta tatgtatgtc attaaatcct ttatttttag cacaattttc
    50401 tatatttgat gcagtttctc ctaataagac caatgattta acatatgact ttgcagtttc
    50461 aagaagttca tcaaaattac ttcccttatc catcccccct gctataagta ttataggtct
    50521 atcataagac tgtatagctt ttatagtgga atctggatta gttccctttg aatcatttac
    50581 atatattaca ttgtctaaat ttcttacaaa ctcttgtctg tgttccactc ctttaaatgt
    50641 cataagtaca tcctttataa cttctaaatc aattttgcag acatatgcta ttgctatagc
    50701 tgccatacaa ttttctaagt tatgacctcc aggtaaactt acatcatctt tatttagaaa
    50761 tttgatttta tcatctatat ctattattat gtcattattt tctaaatata ctcctccatt
    50821 tactttttta gttcttgaaa agtaaattac tttggcattg catttatcag ctaaagattt
    50881 tactatctca tcatcatagt ttaatacaca gaaatcctca acagtttggt tcataaatat
    50941 atttgccttt gcttctatgt atttttccat agtatggtgt ctatttaaat ggtcctctgt
    51001 aatatttaat attgcgctta ctttaggtct aaattcatct atactctcaa gctgaaaact
    51061 gcttagttca gttactaaca cagactcttc acttgaagtt tctatagtat ctattacagg
    51121 atttcctata ttaccaacta catatgtatc tcgttttgct cttgaaaaaa tttctcctac
    51181 caaactagtt gtagttgtct ttccatttgt acctgtaatc cctataaaaa ttggattatt
    51241 cgatagttta aaagctaatt ctacctcacc aattatatac ttgcctgaat tttttaattt
    51301 caatatgaat ggtaggtcta gtggtacacc tggagacact acaaccatat ctatatcatc
    51361 tacatcttct gggtgatgtc ctaatatgta ttttatgtca tttatagatt tcaattcatc
    51421 aagtatattt ctcaatttat tttcatcttt tatatcattt acgattatac ttgcacccaa
    51481 tttatctaaa tgtttaattg tagatatacc agtttttgca agtcctacta ataaaacttt
    51541 ttttcctttt aagtccattt ttcattcccc ctagccctaa aatactgcta ttatacttat
    51601 ccaagctaaa acaactgtta taatccaaaa aataaataca actcttgttt ctggccaacc
    51661 acactgttca aagtggtggt gtattggagc catcttaaag aatctctttc ttgttgcctt
    51721 aaaatatcca acttgaatca aaactgatag agcctccgca aaataaattc ctccaattat
    51781 aggtattata agcactgagt tagttagaac tgaaaatgct acaactgcac ctcctaatgc
    51841 cattgaacct gtgtcgccca taaacactct agccggatat gaattaaatc ctaagaaacc
    51901 taaacatgct ccaacagttg ctgctgctaa aactgcgact tctgtatttc cagcaataga
    51961 acttgcaaat agcataaaga atacagaaac tattaatgtt actcctgatg ctaatccatc
    52021 taatccatca gtcaagttta cagcatttac tattgctacg attataaaca tcattattgg
    52081 tatatataat attccaacat ttatcacaaa atctgtaaat ggtatcataa gttgtgaagc
    52141 acttgatgat gatgtatact gataaaaagc aacataaaaa gataatgcaa cttgaaggat
    52201 tattttttga tatgctttta gtccaagtga tctttttagc tttattttta taaaatcatc
    52261 taaaaagcca ataaaaccaa aacctgcaat acatataaga cctactgcca tatcatgact
    52321 aacttttact cttgtaagtc cagttattag aattgcaaca atcattataa ttccacccat
    52381 agtaggtgtt ccgtttttag ctagatgtgt ttggggtcca tcatctctaa ctgtctgacc
    52441 aaatttaaat tttctaagca ttggtataaa tattggtcct attataatta ctattaaaaa
    52501 cgcaatcaat gcggtgtacg taagctctgt tattcctaac atcataaaac tcctctccct
    52561 tgtcaattaa ctcattagtt taagcttatt tatttagata ttcgactatt ttttctaatc
    52621 tcatgcctct tgagcctttt actaaaatag tgtctcctgt ttttactaat tcatttaact
    52681 tgttaaacac atcttctcta ttctcaaagt gatatatatt ggctgaattg aatcctaatt
    52741 gttttgcttc ttctccaata aacactgaat tttcacctat agttattata atatcagtat
    52801 tattcataga tgattttcca actaatctat gaccatactc tgaaatttca cccatttcta
    52861 gtatatctcc aagtatcgct acccttctac ctttatatct tccaagtatc tttaaagcag
    52921 catccataga atctggacta gcattatatg aatcattaat tattgttaat ttttcatttt
    52981 ttattatgtc tagtctcatt tttgtccctt taaaattttt taaacctttt tttatataat
    53041 ctaaagaaat atttagacac aaaccaacta atatagcaga cattgcatta tatatattat
    53101 gttctcctat tgttggtata aatatttcct cttttttccc atttattaca catataaatg
    53161 ttaaactatc ctcttccata gtataacttt cacaataaac atcattgttt ttattaaagc
    53221 caaatgtttt taacttatat acatgttctt tttcttttaa tgtagataaa aacttatcat
    53281 ctccatttac aattaaagta tttgtttcat caaagtttga agtaatctcc atctttgctt
    53341 tcaaaatacc ctcttgtgaa cctagttttt ctacatgtga taatcctata ttcgatatga
    53401 ctgcaatttt aggatttact atatccacta aatattttat ttcattgaat ccagacatac
    53461 ccatctctat tacagcacac tcatgttctt tatttagatt aaataaagtc aatggaactc
    53521 caaaatgatt atttaagttt ttttcatttt ttagtatatt taactttgca gatatggcag
    53581 agtaaatcat atctctagta gtagtttttc ccacactacc tgtaacacct ataaatggaa
    53641 tatcaaattt ttctttatag tattttgaaa tatctcctag agccaaagat gtatctttta
    53701 cctctattaa atttatatcc gaactttcta attttattcc attactttca ttttttataa
    53761 aagttttaca tccactatca tatgcagact tcataaatgt atgcccatct aaattttctc
    53821 caactatggc tacaaatgca ttttgggcat ttgcctccct gctatctata actatatcac
    53881 ttacacagtc attataatct ccatatatta acttcccatt agttgcaagt actaactctt
    53941 ttattgttaa gcactccatt attaaactcc tcctttaaaa ccttagctat attctcttag
    54001 caacaaaagg caatgtatac cgttatatat acattgccta cgataactct taactttata
    54061 attatatact atatttctgt ttttttgaag atgtttatgg gtatatgtta attatctgtt
    54121 actttttagt tttcaaaata tagggttata cttgagtctt cattaacttt tactccaggt
    54181 tttgggaata tatcctttac ctttgtatct tctggaagtt ctatatctgc atctaagtct
    54241 ggttttaact tcgcatcctc caatgccttt acagcatctc ctatttttag atttcttaca
    54301 tctggcactt taacttgctt cttttcatac tcagcttttt cttcttcttt atatactggt
    54361 ttaactccca aatattttaa agagtcattc attatttctt tcactattgg tcctgctgta
    54421 gtactaccaa atgcacttac tcctgttggt tcgtcaacaa tagcaagaac tactatttgc
    54481 gggtcatcac aaggtgctat tccaacaaat gaacatatat attttcctgg agcatactta
    54541 ccatctataa ctttttgagc tgtacctgtt ttaccaccaa gtctataacc tggtatatat
    54601 gctatctttc ctccaccttc agttacaact gattctgcaa tttctaacat ttttttagat
    54661 gtttctttag atataacact tcttactttt tttggtttta ctgtttcagt tatatttcct
    54721 ttattgtcag tataagattt taccactctt ggttgcatta aatctccacc attagctatt
    54781 gatgatatag ctgttataag ttgtattgga gttactgata tagattgacc aaatgatatt
    54841 gtagccaatt caactggtcc tacatttttt tcgttataca gtattccctt agcttctcct
    54901 ggtaaatcta tacctgtctt atccataaga ccaaatgatt ctatataatc atacattttt
    54961 ccaacaccta atctgcttcc taactcaaca aatactgggt tgcaagagtt ttgtactgct
    55021 tgtttaaatt cttgtgttcc atgaggtctg taatgtctcc aacatttaat ctttcttcct
    55081 ccaacagtta cactacctgt acatgtgaat ttttctccat ctttaattac tccttcttca
    55141 agagcactgg aagatgtaat aagcttaaat gtagaacctg gttcatatgt atcacttact
    55201 gctggatttc tccacatttg ataatagcct tttattttat ctttatcatt atatttttca
    55261 agttcttctt gataatatgg atatattggc gttcttgagt cattagggtc ataatctggt
    55321 tttgatgcta aagcaagtat atcacctgtt ttagggttca tagctattgc agtaaccttt
    55381 tttgcattat ttaactcata agctttctgt actgcttttt cagtatagtg ttgaattact
    55441 tcatctatag acaacacaag accattacct tgtacaggct cataatattt ttccattcct
    55501 tgtggtattt ctcttcctga agcatcagta cttactataa gttttccagg ctttcctttt
    55561 aactttttat catattgcat ttctacacca gatatacctg ttgcatctga agaagtatgt
    55621 cctaacacat atggtgcaaa attcccatat gggtaatatc tttgattatc ttctgccacc
    55681 catattccac ttaatttagc atctcttatt tgacttgcct tatcatcatc tatccatctt
    55741 tttactttta caagagccat attttttttg cttattaaag cgtaaatatc tttataatct
    55801 tcatccaaaa tctcagctac cttttcggct gcttcttttt tatcttctac ttccacaggt
    55861 ttacaccaaa cagtatactt agttacactt actgctaact ctttcatatt tctatcatat
    55921 atagttcctc ttttgggttc tattggtata tctcttgttt gttgttctag tgcttttgta
    55981 ctcaaccaat ttcccttcat aagttgtaga tacccagtcc taataacaag gcaaaaaaac
    56041 aatgcacatg ctagaataag aacgagtacc agccttttct tacttatcct ctttactttt
    56101 ctcaaatcta ttccccctag ttatccacat ctatatactt tatttgtcca tcttttggat
    56161 agtccatatt gagcaattcc ttagccttta gctcaatttc ttttattgat gttttttcag
    56221 tttcttttgc ctttagttcc tctaatacta tctctttttt tcttaaatct ttttgtaaat
    56281 aatagatatc gtacttcatt tccgagattt ttgaataacc acatagattc aatattatta
    56341 ctgaaaaagt tgcaatcagt aaaatgcatt tagctcctat ttttttctta tttctctttt
    56401 tataattatt ctttttttta tttttatatt catttagatt atgtatttta taattttttt
    56461 tcaaattctc accccgatag tatatagttt aaattttcct ctatactata tttgttatta
    56521 atcatatttc ttcctctaaa atttcatcgc taaattaata cactattaca caatctaatt
    56581 gtattttttt ctatacacaa caaaaaccta taaattcact tatactttta tatgaatcta
    56641 taggttagat attaatttct atttattttt atcacttcta gtatttttag ataatttctt
    56701 ccataaatat aatatgtaac tagctatcta ctctctgatt cctaagttct ccattatatt
    56761 acttaatacc atctatatct atatttttat atctaaattt tcattatata tctcaataac
    56821 aaagaacatt tcttatagtg ttaattgttt tttaat
//
LOCUS       DAEOSP010000002        52785 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.002, whole genome shotgun sequence.
ACCESSION   DAEOSP010000002 DAEOSP010000000
VERSION     DAEOSP010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 52785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52785
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.002"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            269..514
                     /locus_tag="KRH54_000053"
     CDS             269..514
                     /locus_tag="KRH54_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG8577739.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            549..1274
                     /locus_tag="KRH54_000054"
     CDS             549..1274
                     /locus_tag="KRH54_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG8577740.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            1296..2609
                     /locus_tag="KRH54_000055"
     CDS             1296..2609
                     /locus_tag="KRH54_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG8577741.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKIYGENLKKIV"
     gene            2815..3354
                     /locus_tag="KRH54_000056"
     CDS             2815..3354
                     /locus_tag="KRH54_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8577742.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            3443..5428
                     /locus_tag="KRH54_000057"
     CDS             3443..5428
                     /locus_tag="KRH54_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HBG8577743.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGIKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            5652..6620
                     /gene="spoIIIAA"
                     /locus_tag="KRH54_000058"
     CDS             5652..6620
                     /gene="spoIIIAA"
                     /locus_tag="KRH54_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBG8577744.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            6610..7131
                     /locus_tag="KRH54_000059"
     CDS             6610..7131
                     /locus_tag="KRH54_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBG8577745.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            7148..7345
                     /gene="spoIIIAC"
                     /locus_tag="KRH54_000060"
     CDS             7148..7345
                     /gene="spoIIIAC"
                     /locus_tag="KRH54_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBG8577746.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            7361..7747
                     /gene="spoIIIAD"
                     /locus_tag="KRH54_000061"
     CDS             7361..7747
                     /gene="spoIIIAD"
                     /locus_tag="KRH54_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBG8577747.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            7832..9037
                     /gene="spoIIIAE"
                     /locus_tag="KRH54_000062"
     CDS             7832..9037
                     /gene="spoIIIAE"
                     /locus_tag="KRH54_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBG8577748.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            9097..9714
                     /locus_tag="KRH54_000063"
     CDS             9097..9714
                     /locus_tag="KRH54_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBG8577749.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            9726..10280
                     /locus_tag="KRH54_000064"
     CDS             9726..10280
                     /locus_tag="KRH54_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBG8577750.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            10307..10996
                     /locus_tag="KRH54_000065"
     CDS             10307..10996
                     /locus_tag="KRH54_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBG8577751.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDESDSSKTEDSSNIDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            11106..11468
                     /locus_tag="KRH54_000066"
     CDS             11106..11468
                     /locus_tag="KRH54_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG8577752.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            11553..12059
                     /gene="nusB"
                     /locus_tag="KRH54_000067"
     CDS             11553..12059
                     /gene="nusB"
                     /locus_tag="KRH54_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBG8577753.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            12043..13113
                     /locus_tag="KRH54_000068"
     CDS             12043..13113
                     /locus_tag="KRH54_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBG8577754.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGYNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSVKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            13110..14315
                     /locus_tag="KRH54_000069"
     CDS             13110..14315
                     /locus_tag="KRH54_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBG8577755.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            14317..14532
                     /gene="xseB"
                     /locus_tag="KRH54_000070"
     CDS             14317..14532
                     /gene="xseB"
                     /locus_tag="KRH54_000070"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBG8577756.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            14535..15422
                     /locus_tag="KRH54_000071"
     CDS             14535..15422
                     /locus_tag="KRH54_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBG8577757.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            15452..15889
                     /locus_tag="KRH54_000072"
     CDS             15452..15889
                     /locus_tag="KRH54_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBG8577758.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            15923..17788
                     /locus_tag="KRH54_000073"
     CDS             15923..17788
                     /locus_tag="KRH54_000073"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBG8577759.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            17921..18733
                     /locus_tag="KRH54_000074"
     CDS             17921..18733
                     /locus_tag="KRH54_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HBG8577760.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            18753..20432
                     /gene="recN"
                     /locus_tag="KRH54_000075"
     CDS             18753..20432
                     /gene="recN"
                     /locus_tag="KRH54_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBG8577761.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            20646..21512
                     /locus_tag="KRH54_000076"
     CDS             20646..21512
                     /locus_tag="KRH54_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577762.1"
                     /translation="MNKKDTMHLIFTIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QEIMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECVIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(21605..22096)
                     /locus_tag="KRH54_000077"
     CDS             complement(21605..22096)
                     /locus_tag="KRH54_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8577763.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(22157..22597)
                     /locus_tag="KRH54_000078"
     CDS             complement(22157..22597)
                     /locus_tag="KRH54_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8577764.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            22758..23816
                     /locus_tag="KRH54_000079"
     CDS             22758..23816
                     /locus_tag="KRH54_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG8577765.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            23958..24782
                     /gene="spo0A"
                     /locus_tag="KRH54_000080"
     CDS             23958..24782
                     /gene="spo0A"
                     /locus_tag="KRH54_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBG8577766.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            25195..26316
                     /locus_tag="KRH54_000081"
     CDS             25195..26316
                     /locus_tag="KRH54_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577767.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            26334..27209
                     /locus_tag="KRH54_000082"
     CDS             26334..27209
                     /locus_tag="KRH54_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBG8577768.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFDILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSAKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            27233..27925
                     /locus_tag="KRH54_000083"
     CDS             27233..27925
                     /locus_tag="KRH54_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG8577769.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            27933..28757
                     /locus_tag="KRH54_000084"
     CDS             27933..28757
                     /locus_tag="KRH54_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG8577770.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            28762..29829
                     /locus_tag="KRH54_000085"
     CDS             28762..29829
                     /locus_tag="KRH54_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBG8577771.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQKAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            29831..30367
                     /locus_tag="KRH54_000086"
     CDS             29831..30367
                     /locus_tag="KRH54_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG8577772.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            30435..31040
                     /locus_tag="KRH54_000087"
     CDS             30435..31040
                     /locus_tag="KRH54_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBG8577773.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            31052..31966
                     /locus_tag="KRH54_000088"
     CDS             31052..31966
                     /locus_tag="KRH54_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG8577774.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            32008..33180
                     /locus_tag="KRH54_000089"
     CDS             32008..33180
                     /locus_tag="KRH54_000089"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBG8577775.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            33227..34039
                     /locus_tag="KRH54_000090"
     CDS             33227..34039
                     /locus_tag="KRH54_000090"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBG8577776.1"
                     /translation="MFEKIAQSSKFINSKSDIKPKIGLILGSGLGDLANDIEDPVAIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            34243..35568
                     /locus_tag="KRH54_000091"
     CDS             34243..35568
                     /locus_tag="KRH54_000091"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBG8577777.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            35781..36860
                     /gene="mltG"
                     /locus_tag="KRH54_000092"
     CDS             35781..36860
                     /gene="mltG"
                     /locus_tag="KRH54_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBG8577778.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            37032..37706
                     /locus_tag="KRH54_000093"
     CDS             37032..37706
                     /locus_tag="KRH54_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBG8577779.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            37693..38940
                     /locus_tag="KRH54_000094"
     CDS             37693..38940
                     /locus_tag="KRH54_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG8577780.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            39012..40676
                     /locus_tag="KRH54_000095"
     CDS             39012..40676
                     /locus_tag="KRH54_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBG8577781.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(40773..41057)
                     /locus_tag="KRH54_000096"
     CDS             complement(40773..41057)
                     /locus_tag="KRH54_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG8577782.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(41080..42597)
                     /locus_tag="KRH54_000097"
     CDS             complement(41080..42597)
                     /locus_tag="KRH54_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG8577783.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKASDIDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            complement(42722..43543)
                     /locus_tag="KRH54_000098"
     CDS             complement(42722..43543)
                     /locus_tag="KRH54_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG8577784.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIKIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETE
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKYLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            43734..45161
                     /locus_tag="KRH54_000099"
     CDS             43734..45161
                     /locus_tag="KRH54_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HBG8577785.1"
                     /translation="MKIKKLLTIGLSLSIFIASCPISANALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMSDGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSIDKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSVAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKIVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTAAKNNAIVLVDNDSNKSV
                     LKSTTKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYK
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKVYN"
     repeat_region   46117..46475
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=46117..46145
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaag"
     gene            complement(46762..46986)
                     /locus_tag="KRH54_000100"
     CDS             complement(46762..46986)
                     /locus_tag="KRH54_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8577786.1"
                     /translation="MDVNQRIKYIRENMNSSQKELLEKTNINTSVMNRIESGERPTRD
                     DGLIIFAKIFGLYTDYILGLSDTEKLNIGN"
     gene            47697..47852
                     /locus_tag="KRH54_000101"
     CDS             47697..47852
                     /locus_tag="KRH54_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBG8577787.1"
                     /translation="MALDVKIEVKIIKDVVILNITHWKKETSLLTVKIARSQGDLSYV
                     KYFANES"
     gene            48098..48349
                     /locus_tag="KRH54_000102"
     CDS             48098..48349
                     /locus_tag="KRH54_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577788.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            complement(49559..49942)
                     /locus_tag="KRH54_000103"
     CDS             complement(49559..49942)
                     /locus_tag="KRH54_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577789.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
     gene            50069..50413
                     /locus_tag="KRH54_000104"
     CDS             50069..50413
                     /locus_tag="KRH54_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577790.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
     gene            50596..50811
                     /locus_tag="KRH54_000105"
     CDS             50596..50811
                     /locus_tag="KRH54_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577791.1"
                     /translation="MHINQTGFISNELGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFELIATLVRGGSSWAFNFIVQP"
     gene            complement(51796..52113)
                     /locus_tag="KRH54_000106"
     CDS             complement(51796..52113)
                     /locus_tag="KRH54_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577792.1"
                     /translation="MNFRNIYLILMGLGIIILTTIISLIEIKIGFCNEKYFNKLESKY
                     GNIDRKRTIKLEVLYRYVTGFEYIAIGLFTKRLDITIIAIILVATITVILYYLVRKRY
                     ITI"
     gene            complement(52423..52785)
                     /locus_tag="KRH54_000107"
                     /pseudo
     CDS             complement(52423..52785)
                     /locus_tag="KRH54_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        21004 a         5522 c         9076 g        17183 t
ORIGIN      
        1 ttatattatg tcagaaagaa tattgtaatt tataattaaa tattaatagg tttttaaaat
       61 aggattagat aggtaatagg ttaagttaga ataacccatt acctattttt atttgtaaga
      121 aaagtgtaca tagaattaga agtaaaaaat acaactaata aaactattga aaatactaga
      181 taaataataa ataaaaataa ttatagattt tggcatacat attgctaatt atagagataa
      241 gacgacaaaa taattgggag gtttaatgat gaaatacaaa acaacaggga cttgtgctac
      301 agaaattgaa tttgaagtaa aagaaaataa agttacaaat gtaaacttta taggtggatg
      361 tgatggaaat ttaaaagggc taaaagtatt agttgaaggc atgaatatag aagatgtaat
      421 acaaaagtta aaaggaatag agtgtaaaac aaagccaaca tcttgcccag accaactgtc
      481 tctagcattg gaaaattata tgaatataaa gtaatacata aaacaatcga ttagttagga
      541 gtttaattat gaaaccaata atagggattt taggaaattt aataataatg gaaaatggta
      601 tgtttccagg tcttgaaagg tcatatgtaa ataatgatta tataaatgca gtgctaaaag
      661 gtggcggtag tccagtaata attcctgtaa atacagataa agaagttata aaaaaacaga
      721 ttgaaatggt tgatggagtc ttaatatctg ggggatggga tatcaatcct caactgtatg
      781 gagaagaaac aagagaagaa acgacattta tatatccaga agtggatgaa tttgatttaa
      841 ttgcaataag tatagcatta gaacttaaaa agcctatatt aggtgtatgt aggggactac
      901 aaattttaaa tgtaagttta ggaggaactt tatatcaaga taataatctt atagaaggaa
      961 gttatataaa acatacacaa tcttctaaaa gacatgtagc tactcataaa gttgatgtta
     1021 aagaaggaag tattttagag ggaattttag gaaaacaatt acttacaaat agttatcatc
     1081 atcaaagtgt caatcagctt ggaaaaggat taaaagccat agcatactca aaagatggta
     1141 taatagaagc tattgagaaa gaggatgaaa attttgtagt aggagtacag tggcatccag
     1201 aaatgatggt agattactgt gataaaatgg aaaaactatt taaatatttt ataaatattt
     1261 gttcgaattt ataatatacc ttggggggat tgaacatgaa cgaaaagaac aaaatgggat
     1321 taataagtat tatactttta ggtattaatg cagtagtagg agctggagtt tttctattac
     1381 caggagatgc aatgaaatca tttggtgtag caagtatatt tgtatatata tttgatatgt
     1441 tgttagtttt atctatggca ttctgttttg ctgaggttgc aggtaaattc aataaaaatg
     1501 gtgctgccta tgtatataca aaagaagctt ttggagattt ttgtggtttt gaagtaggtc
     1561 ttatgaaatg ggtaatagga tgtatttctt ggggagcttt aatagtaggt tttcctacat
     1621 cattatcagc agtatgggct ccagcaggtg aaccacatat acaaaaaatt ataattgtag
     1681 ctatgatagt tgggcttaca attataaact tattaggtgt atctctatca aaaattgtac
     1741 aaaatgttat aactgtaggt aaactaattc cattaatact atttatagga ataggtatat
     1801 tttttataaa aggagttaat tttacttctt caacaatggt acctccagga gctggagcaa
     1861 cagagtttgg agcagcagca cttttaatgt tttattcatt tacaggattt gaatctatag
     1921 cagttgcagc agaagatatg gaaaatccac aaaaaaatat accaatagca attataagtg
     1981 taatagtaat tgcatcaata atatatatat taaatcaagt tgtatgtgtt ggtatactag
     2041 gagattcgtt atctagtaca tctacaccag tggcagatgc tgcaagaata tgctttggaa
     2101 atatgggagc tggattggtt acctttggaa cattggtttc tgttggtgga atttgtatgt
     2161 gtggtgcttt tgtaaatcca agaagttgtg tagctttagc tgatgataaa atgttaccga
     2221 gaatatttgc aagaaaagat aaaaaaggta caccatatgt agcgataata gctactatgt
     2281 taatcactat accaatagct ctatctggaa gttttgctga attagcagca ataagtgcag
     2341 ttgctagatt catacaatac ataccaacat ctttatccgt attagtgttt agaaagaaaa
     2401 gaccagaatt agtaggtact tttaaaactc cttttggagc agtgattcct ctaattgctg
     2461 tatgtgttgg aacatggttg ttattccaag catctatgca tcaattaatt atgggattag
     2521 gagctttggc aattggagta ccattatact ttataatgaa gtcttataat agaaaaatct
     2581 atggtgaaaa tttaaagaaa atagtttaat tatgttataa caatttattg agttaaaatt
     2641 agacattggc agaaaattaa atacaaataa gtatctacaa ggctatatta aaatattatt
     2701 aattttttat tttaatatag ccttatattg ttatcaataa atgaaaaata ttgtaacaac
     2761 agaagaacaa tagtattatg tttatagaca aaaaacttat taggaggtaa atttatgcca
     2821 acaataacat taaaaaatgg tgtagacgta cttattagag aaggtgtaag agaagatgct
     2881 caaagcatta tagattttta taatgaagtg ggtggagaaa ctcattttct gtcatttgga
     2941 aaggatgaat ataaaatatc tttggaggaa caagaaaatg ctatagaatc agcaaaagca
     3001 tctgataatt ctgttaaact gattgcattt atagatggtg aaatagttgg gattgcaacg
     3061 attgattcta atcaaaaagc aaaaggaaag catgttggag tattaggaat tgttgtaaaa
     3121 gaaaaatatt ggggaattgg attgggtaaa aggcttatgt tagaccttat agaatggtgt
     3181 aaatcaaatg gaataaccaa gaagataact cttgtcacta atgaagaaaa ttataatgct
     3241 ataggattat acaaaaaagt tggatttgaa gtagaaagca tactaaagaa agaatgttat
     3301 tataatggtg tttatactga tttaatagga atgtcacttt tattgggaat ataattagtg
     3361 tacattttat ctcgaaaaaa gtatattaag tatggtataa ttaaaaaaga atataactta
     3421 atataatttt ggaggacgaa ttatgaaaaa tctgtgtaaa atatcagatg attatttaaa
     3481 cagcaagctt aagtatttaa agttattatc aaaacaatat ccaagtatat caaaagcaag
     3541 tacagaaata ataaatttag aggctatatt aaatcttcca aagggaacag agcattttat
     3601 aacagatgta catggtgaat atgaaccatt tgtacatgta ttaaaaaatg gttctggtgt
     3661 aataaagaga aaaatagaag aattgttttc aaatacaata agagacagcg aaaaaaaaat
     3721 gttagcaact cttgtatatt atcctgaaca aaagttagac ctaataatta agcaagagga
     3781 aaatatagat gatttttata gaataaatat ttatagactt atagaacttt gtaaatatgc
     3841 ttctagcaaa tatactaggt ctaaagttag aaaactgtta ccagaaaatt ttaaatacat
     3901 aatagaagaa cttttgcatg agcatgttaa aagtgaacat aaagaagagt attataaaag
     3961 tatagttgaa acaatagttg acataggaat agctaaggag tttataattg ctatttctac
     4021 agttatacaa aaattggttg tagatagact tcatgtaata ggagatattt atgatagagg
     4081 tccaagacca gatataattg tcgataagct tattgaacat cattgtgtag atattcaatg
     4141 gggaaatcat gatatattgt ggatgggtgc agcctcaggt gaaaaaactt gtatagcaaa
     4201 tgcactgagg atatcagcta gatatgcgaa tttagacatt gtagaagaca tatatggaat
     4261 aaatctattg cctttagcca cttttgctat agaaatgtat aaagatgacc cttgtaaaga
     4321 atttattcca aaggttaatg accaaagtgt aacaacaaca gaaaagtctt taatggcaaa
     4381 aatgcacaaa gcaataagta taattcaatt taagcttgaa ggtgaggtta tcagacgaag
     4441 acctgaattt gaaatggaac atagactctt gcttaatatg ataaactatg atgaaggtac
     4501 gattaattta aaaggtaaaa catataaatt aaaagatact tatttaccta caatagacaa
     4561 aaaagaccca tataaattaa ctatggaaga aagaaatgtt attgataaat tagtatcttc
     4621 atttagaggt agtgaaaaac tacaaaaaca tgtttcattt ttgttttcta aaggaagtat
     4681 atatttaaag gctaactcaa atttactgat tcatggatgt gtaccgttaa atgaagatgg
     4741 aagctttatg tctatgaata taatgggaaa agagtataaa ggtaaggctc ttatggataa
     4801 gatggagtct ctagcaagag aaggattttt ctttaaagat aaggcagaag aaaaactgta
     4861 tggcatggat attatgtggt atttatggac tgggaaatgt tcatcattat ttggtaaaga
     4921 tgatatgact acatttgaaa gatactttat agctgaaaaa gagactcata aagaaaataa
     4981 gaacccatat tttaaactta gagaaaatga gatggcttgt aaaagattat ttgaagaatt
     5041 tgatttagaa cttgatgaat ctcatataat aaatggtcat gttcctgtgg agagtaaaaa
     5101 tggagagagc ccaataaaag ctaatggtaa aattctcgtt atagatggtg gtttttctag
     5161 ggcttaccaa aaaactactg gtatagctgg atacactttg atatacaatt ctagaacttt
     5221 gcaattagta tcgcatgaac catttaattc tgctgaagaa gccattgcaa atgaaagtga
     5281 tatcttatcg acaactgttg tagtagagca taaggcaaag agaaaaatgg tgagagatac
     5341 tgatgaagga ataaagatac aagaggaaat agaagattta aaactacttt taatggctta
     5401 taagaaaggg ctaataaaag aaatgtagat gttatagagg cacttcatga ttgaagtgtc
     5461 tttttgttaa tacttattaa atatattata aagattaata atagtagttt atattatact
     5521 tactaagtga atccaagata aatgggttca ctttttttat ttataaacat tattaaaaaa
     5581 atatagatgt tttggttata ttccttgtcc tttataaata gaattatata aacaaaggag
     5641 gaggacaaga gatgaataaa ctttctgatg aaataataaa ctctctttct acaactataa
     5701 gagaaaaggt agaaaaagtc tcaaacaata atctgaacat agaagaaatt aggttacgct
     5761 cacaaaaacc attgatacta aatgcaaact ctaaagatta tttttacaat caaaaaacta
     5821 tgacacttga tttaaatcaa caaaactcat atgtagtaac aagagaagat gtagagcaaa
     5881 cttttcaaat aatatgtaaa tattcaatac attcatttat ggatgacata aaaaaaggat
     5941 tcataacttt gagaggaggt catagagtag ggttagttgg aaaagcaata gtagaagatg
     6001 gacaagtcaa aaatataaag catatatcat ctttaaatat aagggtatca agggaaatta
     6061 ttggatgctc ggataagatt ttaagtcata tcataaaggg aaaaaatcag attaataata
     6121 ctttgataat atcaccacct caatgtggaa aaacaacttt aataagagat atagtaagaa
     6181 atttaagtaa tggtaatgaa gattatggat ttaaaggttt aaaagtggct ttagtagatg
     6241 agcgtaatga gatagcagga gcttatctag gtgttccaca aatggatgtg ggaataagga
     6301 ctgatataat agaaacttgc ccaaaagact tgggtattac aatgctttta aggtctatgt
     6361 caccaaatgt tattgtgaca gatgaaattg gaagtgaaaa agaaataaag gcattataca
     6421 cagcattaaa tggaggtata ggattaataa ctactgtgca tggagattca atagaggata
     6481 tacaaaatag aaaagaatta aatagacttc tggataaaga gttatttaaa aaggttataa
     6541 ttctttctgc aaaaagagga gcaggaacaa ttgaaaaaat ttatgattta gaagaaaaga
     6601 gatggtattt tgcaaattaa aataattatt atagctttct tgataggaag tagttatttg
     6661 ataggtgaac aaatatacaa aacttatact cgtagacata aacaattaaa cgatttaata
     6721 agagttttag aaattttgag gatggattta tcttttggac tttatacatt agaagaaatc
     6781 tttaatagga taggtggaaa taaagagttt tgtttttgga aatttttcta tcaaatatca
     6841 gaaggacttc acaatgaaca aagtaaaact ttggaaataa taatatcaga aaatattgat
     6901 gtcttaagta aagaaactta tctaggcaat aaagaaattg aggaactgaa aaatctaata
     6961 cttactttag ggaaaagcga tatagagtca caacaaagaa tgatagattt atctatagaa
     7021 aatttgaaaa aacaaaccta tgagacaaag gaagatataa acaaaaaagg cgtattgtat
     7081 aaaaaattag tgacattcat aggtataggt atatgtatta tattgattta atatggggag
     7141 gtttattatg gaaatatcat tgatattaaa ggttgcaggt gtcggtattc taatatcagt
     7201 attgaatatg atattggaaa aaacagatag aaaagattgg gcaggtctta ctacattagc
     7261 aggtgtaata atcgtattgg gtatggtaat aacagaaata agtgacttgt ttaatactgt
     7321 aagaacaatg tttcaacttt actaaagagg agggagtaat ttggaaataa tgcaattaat
     7381 aggaattgca atcatttcaa ctacactctg tttagtaatt aaaaaagata gaccagagat
     7441 agcaaatttc atagctataa taactggagt aataatacta ttgtcagtta tgtttaagct
     7501 aaattttata gtagatagta tacaagattt ggctaacaag gctaacatcc cgactatgta
     7561 tatatcatta ataataaaat taattgggat agcatactta atggaatttg cgatacaact
     7621 ttgtaaagat tgtggggaag gaaacattgc ttcaaaacta gaatttggag gtaaaataat
     7681 agttatgtca atgtctttcc ctatactttt gtccattgta gaaatggtgg tgaatataat
     7741 tccatagatg tgtatcttgg aatgaatata agtatattag agagctttcg ttgtattgag
     7801 gtttatagag tgaagagagg catggttgct tatgaaaaaa agatttttac caatgattat
     7861 aggtttttta tttactttct tttttgtaaa tatctttgct attatgatat ttgctaatga
     7921 agtaccaagt agtgaagata aagaatatgg tgaaacaaaa aatagcatag ataagtatat
     7981 agatggtcaa ttagacaagc tagatataaa tgagattcaa gactatataa ataaagaaat
     8041 agttattaat gatgttaatt taaaatcatt tgtaaaagat ttaattagtg gagaaaaaaa
     8101 tatcttagat ttattcaata aagatggatt gaagatattg atgtttgatg aatttaaagc
     8161 aagtttaaag gtagtagcag taattttggt actggctctg ttatcatcta ttttaaagag
     8221 tctggaaaac tctttttcat caggtgcagt aagtcaaata gcaacatata taatatttat
     8281 cacaatggta tcacttacac ttattggttt taaagatgta ttacagattt gctatgatgc
     8341 aatagaccat actgtaggtc ttatgcaagt tataatgcca atattaataa catttttact
     8401 tttaattgga ttcccaataa cgtcgactac tttgaatcct atcttcatag gaggagtaac
     8461 atttataaat gtatttttta aaaattttct atttgtatct attactgttg catttggaat
     8521 tttaataata aacaatttat caaaaaatat aaggctaaag agatttttct cctttgtaaa
     8581 acaaataaat tatgtttcta ttggagctat gtttactgtt tacttaggtc ttgtatccat
     8641 acaaggactg tatgtaacca gttttgataa attcagtgta aagactgcta aatttgctat
     8701 aggtaatttt attcctgttg taggtggatt tgtatctgac tctgtggata ttttattatc
     8761 ttcatctcag cttataaaaa atatatttgg aggaataggg cttatcttac ttgttggaat
     8821 ttgcttactt ccagtaataa aaattttatc agtaatagta gtgtacaaat tagcagcaat
     8881 aattgtagag cctgttggag aggatggaat atctaatttt ttaaatgaag tggcaaattt
     8941 aatgataata atgctagcat cagtaattgc tataacagta atgttctttg taactgtagc
     9001 tatcttgacc tcgataagtg tcgtaagtca agggtaattt atatatcagt gaaaaataat
     9061 tacatttaat ctaatgtttt tagagaggag gatgctatgt tagagggcat aaaggcatgg
     9121 atagttagtg ttttgatagg agcatttata gtaaatatag tagatatgat tttaccaagt
     9181 tctaagataa aaccttatgt caacctagtt ttaaacttta tgtttgtatt tattgtaata
     9241 actccagtag taggattttt ttcaaaagat atgagtttag aagatagaat attaaaatct
     9301 atgggaaact acaacaagca gtatgtagat agtacaaatg ctttggcaaa agagactgga
     9361 aataatagtc tatccaaggg atatgaggat gggttaaaag aagtacttaa attaaaactt
     9421 gatgaatatg gttatgattt agaagatata gagcttaatg gggcaaatat aaataatata
     9481 aaaataaaag aaaagaataa tagtgcaaaa agtaataaca gttccaatga agaaaataaa
     9541 aataatagta ctaaaagaga ggaagaaaac ataaactcta atgacaaaga aaattctaaa
     9601 caagtcttca aaaaaggtac agagtatggg ttgaacctaa atgaagaaaa attaaaaaac
     9661 gacttaataa aggtgctgga tgtttctata gaagatatac aaattgataa ataggagttg
     9721 ggagaatgtt gaaaaactta aatgacaaag ataagagaaa ggtatattca ctaataacta
     9781 tagcaggaat atgtgtggtt tcattagtat tattatcttg ttttcctagt agcaaaagcg
     9841 ataaacaagt tggaaaaaca gagactaata aaaatgctga aaagcaagtc actaaagaac
     9901 aagaaaaaga tgatttagag tcaaaattaa cagcaatact atctaaaatt gatggtgcag
     9961 gagacgttga tgtaatggtt acatttgaat caagtgaaga aatacaacct gcttttaatt
    10021 caaataatac aacagaaaca acagaggaaa aagatgcaca aggtggagaa agaacagtta
    10081 cgacttctag tgaaaataaa acaatgataa cttcaaattc aagtgaccct gtagttataa
    10141 aaactacaga gccaaagata aagggagtga tagtggtagc aagtggagca agtgacccaa
    10201 gtgttaaaga gacactttat agtgcagtac aaacatcatt acaagtagct ggtcatcaag
    10261 tagaaatata ttctaaataa atacatataa ccggggggat aaatgaatga agtttaatta
    10321 taagggaaga ggatttgtaa taataacttt aactgcaatg ttagtggtag taggaacagt
    10381 gaattatcaa ctaagtaaga aatctttatt agaaacatca aaagagttta aggcatatga
    10441 agaagcacag ttgcaaaaaa atactgatga gagtgatagt agtaagacag aggattctag
    10501 taatatagat aaacaagatg gtaaggaaag tgctgatatt gatatagttg atagcaaagc
    10561 tagtaaagta aaagaaaagg caactgagac aagtaaggaa ataaaagctc aattatcatc
    10621 agagaaaaat atgaaaaagg catcttatat tttagatatg aaaatgaata gagaaaaaca
    10681 aagaaatgag ttagttcaag atttaaatga aatgataaat aatccatcta caacagaaga
    10741 atctagaaaa gaagcttcta atatgaaatt aaatatagtt aaaattcaag aaaaagaact
    10801 tcaaatagaa aaccttttga gtacaaaagg gtatgaagaa gcgttagttt atataagcga
    10861 taacaaagtt aatgtagttg taaatgaagc taaattagaa aagaaagatg cagcaaaaat
    10921 atttgattta gttgcagaac aagccaatgt gaagtatgaa aatattaaac ttacaaataa
    10981 taatagtaat aagtaaagta taaacctcag aattaactga ggttattttc ttgcaaagta
    11041 gacttgtatt tggtattatt atacttataa aaatagttcc ttaaaatcag gaggtaagtt
    11101 ttaatatgga agataataaa tttggacaag taaaaatatc taatgatgta atagctacaa
    11161 tagctggttt agcagcatta gaagttgaag gcatagaaac tacagcaaca ttaacagata
    11221 agttgttaaa aaataatgga gtaaaaatac aaatcgaaga agaggatgtt aatttagatg
    11281 taatggttac gataaagtat ggaatgtcaa taccagatac agcttttaaa gttcaagaaa
    11341 atgtaaaaaa tactgttgag acaatgacag gactgaaggt ttctcaagta aatatacaca
    11401 ttcaaggaat cagctttaag aaggataaag ttgataaaga agaagcaaaa gcaactaaga
    11461 aaaattaata ttagccctct gaaaagtcag agggtttttt gctggtaatt gattttatat
    11521 ggataattaa acttaaagga gaattttgat tgatgaaaaa agatagggca caaaagagta
    11581 caacaagaga atatataatg aaacttatat accaaataaa tattaataaa gaagattttg
    11641 aaactttaga agataaagta gataattttt taaaagataa ttcagaacat attataaata
    11701 gatataaaga acttgcactt caatactcaa aaaatacaaa tttaaagtta gaagatactg
    11761 aaattgaaga tgttatagac aaaaaatata taaatacagt atgcaaagca ttgaaagaaa
    11821 atcatgacaa aatagatgag ttaataaata agcatgctaa aaactggaca gtagatagaa
    11881 tgccaaaggt agatgtttct atacttagat tgtctgtgtg tgaaatactt tatttagaca
    11941 ctcctaataa agtatcaata aatgaagcag tagaacttgc aaaaatatat tgtgatgata
    12001 agtctcctaa atttataaat ggtatattgg gaagtgttgt tgatgaaatt gggaaataat
    12061 ataataattg gaattgatac tagctgctat actacttcta tagcagctat ctctttagat
    12121 aagaaagtta tattcaatga aaaaataatg ttagaagtaa gggataattc aaaaggttta
    12181 aggcaaagtg aagctgtatt tcaacatatc aataatttag ggattcttag tgataggata
    12241 aaatctttta aagataagtt taatgtagaa ggagtttgct catcaaaaaa accgagacct
    12301 gttgaaaact catatatgcc tgtattcaat gtaggtcata attttggaaa attattgtct
    12361 agtatatatg gttgtaggtt ttatgagact actcatcaag agaatcatat tgaagcaagt
    12421 ttattaaata gtaaattaaa aaataataat aaatttattt ctgttcatat gtctggtggt
    12481 actacagaga tacttttaac aagcaaacaa gatagtcatc acaatgtatg tgatactaat
    12541 ttaggtaaaa ttgccaaaat aagtattaaa aaagatgata aaagtaagtt atacaataat
    12601 tttggatata atatagatat aattggagga agtaaagata ttagctttgg tcaacttata
    12661 gatagagtag gaattaagct tggatataaa tttccttcag gaaagtattt ggatgaaaat
    12721 gctttaaatt gtaatcttaa aatagaaagt ggtctgaaaa cttctgtaag agatgggtat
    12781 atgaatttat caggattaga aaatcaggta aataagatta taaatgataa tggatataat
    12841 actaatcaaa aagaatatat atctaaattg gtattagatt cagtagttag aaatatgttt
    12901 aaatctttag tgtatttatg tgaaacttat aatgtaaatg aagtagtatt tgctggtgga
    12961 gtttctgcaa gtaagtatat tcttagagaa ttaagcgtga agttaagaaa aaagcatata
    13021 gaagcttatt ttacagaacc acaatattca actgataatg ctgtcgggtg tgctataata
    13081 ggattgaata actttttagg agaaagagta tgaaactaag agctttagat ataagtgaag
    13141 caaactcata tattaaaaga attttaataa acgacccaat actttctaat cttaaagtta
    13201 aaggagaaat ttctaatttt aaggttcata gtagtgggaa tgtatattta tcacttaaag
    13261 atgaaacatc aaagttaaac tgtgtaattt ttaaaagtaa ttttaatcgc aatttaaaat
    13321 tagataatgg tgtaaaaatt atagctaatg gatatatatc agtctatgaa agagatggtg
    13381 cgtatcaatt atacattaat gaaattgaaa tagagggaat aggaaatttg cacatagagt
    13441 ttaatagatt aaaggaaaaa cttaataaag aaggattatt tgaccctaaa tacaagatac
    13501 ctatacctaa aatgccaaac tcaattggcg ttataacatc tccaacaggt gctgttataa
    13561 gagatataat aaatgtaata aaaagaagat atccgaaagt taatataaag ctttatccag
    13621 taacggttca aggagataaa tcagctgaag aaatctgtga agcaattaga tttttcaatc
    13681 atatgaagaa tgttgataca cttatagttg gtagaggtgg gggttcaata gaagaacttt
    13741 ggtcttttaa tgatgaaatg gtagcaagag aggtttttaa ttctcaaatt ccgataatat
    13801 cagcagttgg gcatgaaacg gattttacta tttgtgactt tgtgtctgat atgagagccc
    13861 caacgccatc agcagcagct gagattgcta ctccatcatt ggatgatata aactataaac
    13921 ttggaaacat aaaaagtagg atgagtaagt ctcttacaaa tcaaatagag ctagaccaat
    13981 ataggttaga aacagtattt aataagataa ataactattt agattcgtac acaataaaag
    14041 ataaagttat acaattagac aaaatatatg ataaaataat ttttggaata gaaaataatt
    14101 taaaattaga agatgaaaaa ttagtaaaaa ttggagcatt actccataat ttaagtccat
    14161 tagctactat ggatagaggt tatagtataa ctcaaaaaaa tggaaaagta ataaatagta
    14221 taaaaggatt aaaaataaag gatagtatag atatagtatt aaaagatgga aatttagaat
    14281 gtatgataga taaaattgaa aataaagagg gatagtatga atttaaccta cgaagaagct
    14341 tataaaagat tagaaagtat tttaagtgaa ttagaatcta aaaatgctag cttggatgaa
    14401 tctttaagcc tatatgaaga aggtataagc ctttataaac attgtaataa gcttttagat
    14461 gatgctaaat tgaaaataag taaatttaat caattgggaa tagaagaaga ctttaaaata
    14521 gaggaggaat aaaattggaa tttaaacaat gtctaaaaga aaaagcaagt tttgtagaaa
    14581 aagtacttaa agaatatatg cctaaagaag aagggtatca aaaaactgtt attgaagcta
    14641 tgaactatag cttaagtgct ggaggtaaaa gattaagacc aatacttaca ttggaggctt
    14701 gtaaaatagt tggaggtaat gaagatgaag ctattccttt tgctattgca attgaaatga
    14761 tacatacata ttcactaata catgatgatt tgccagctct tgataatgat gatttaagaa
    14821 gaggaagacc aacaaatcat aaagtatatg gagaagctat ggggatttta gcaggagatg
    14881 ctttgttaaa ctatgctttt gaagtaatgc ttgcaggctc aatcaataaa gaaaatccag
    14941 aaaagtatct aaaagctata aatgaaattg ccaaaggtgc tggtatatat ggaatgatag
    15001 gtggtcaagt agtagatgta gaaagcgaaa ataaacaaat tgaaaaagaa aaattagatt
    15061 atatacatat gaataaaact gctgcaatga tggtaggctg tatgagagca ggagctacta
    15121 taggtggagc aaattctgaa caaatggaag aaataactaa atatgcaaaa aatatcggat
    15181 tatcattcca gatagtagat gatatactag acatagtagg agatgaagct aagttaggta
    15241 aaaaggttgg aagtgatata gaaaatcata agtcaacata cccatctctt ttaggtttag
    15301 ataaatctaa agaaattgca cataatttga ttgatgaagc aaagaaaagt atagaaaaat
    15361 tatctgatga tgtagatttt ttaaagggat tggctgaata tataatagat agagaatact
    15421 aaaatataca gctaaaatcg aggagggaaa aatggatttt ttttcggaga tttttaacaa
    15481 tggggctttg ggaataagtc tgattgcttg tttcttagca caatttataa aaatatttac
    15541 tggtaaggaa aaaagaattg agttatcaag aatacttatt tcaggaggta tgcctagttc
    15601 ccatagttct tttgttacaa gtttagctac agtggtaggt attgaaaaag ggtttaattc
    15661 tactgatttt gctataatta ccgtgcttgc cttaattata atgtatgatg cagcaggtgt
    15721 tagacgggct gttggtaaac aagctactat attaaatcaa atggtggcag atatacagca
    15781 tggaaagcat atagagcaga agaaattaaa agaattaata ggtcatacac cactagaagt
    15841 ttggtttgga gctttacttg gaatagtaac agctttaata ttgatgtaaa ttgaaaattt
    15901 acagaataaa ggtgaggata atatgtataa atatttagat aaagtaaatt ctccaaagga
    15961 tataaaaaat atgagtatag aagaaatgga tttacttgca aaggatataa gaaagttctt
    16021 agtaaaatct gtttcaaaga caggaggaca cttagcttct aatctaggag tggttgagtt
    16081 aacattagca cttcataaag tatttgatag ccctaaagat aagattgtat gggatgtagg
    16141 gcatcaatct tatgttcata aaatagttac tggaagaaaa gattgttttg tatctttgag
    16201 acagtttaat ggactaagtg gttttccaaa ggagaatgaa agtcctcatg atatttttga
    16261 tacaggacat agcagtacat ctatttctat agctacaggt attgcctgtg ctagggatat
    16321 aaaaaaagaa aactatagtg ttatatctgt gataggagat ggttctataa caggaggtat
    16381 ggcacttgaa gctttaaatc aattaggata tatagatacc aatatgatag taatccttaa
    16441 tgataatgag atgtccatag ataaaaatgt tggaggaatg tctaaatatc tgtcaagtat
    16501 tataagaaac tcaacagttg agaaaatgac agatgaagtt gataaaattt taaatgttac
    16561 acaaacaggt gaaattttat ctaagactgc acatagattt aaagataaac taatgtatag
    16621 cttttctcct caggactgtt cattttttga ttcattaggt ataagatatt atggacctat
    16681 agatggccat aatactaaag aactaataga tatattgaga aaagctaaac acaaaaaagg
    16741 gcctgttctt ttacatgtaa ttacgaaaaa aggaaaaggg tacagatttg cagaagaaca
    16801 acctgataaa tatcatggag tatcaaagtt tgatataaag acaggtgtta catctgcaaa
    16861 agttaagtct atgtcaatta gtgttggaga aaaacttgtt gatatggcta atagtaatga
    16921 aaatatagta gcaataacag ctgcaatgcc atctggtaca gggttaaacc tgtttgaaag
    16981 tgcatatcca aaaagatatt atgatgttgg tatagctgaa caacatgcaa caggatttgc
    17041 agcaggtctt gcaaaaaatg gtatgaaacc ttactttgct gtgtactcat catttttgca
    17101 aagggcatat gaccaagtta ttcatgatgt atgcataaca aaaaagccag ttacttttct
    17161 tatagataga gctggattag ttggaaatga tggagaaact catcatggta tgtttgattt
    17221 aagttactta aattctattc caaatattgt agtaatggct ccaaaagaca caagagagat
    17281 ggagcttatg atggatttgt cattaaaatt agattgtcca ctagcaatta gatacccaag
    17341 aggaagtagt tattacctag ataagggaga gtatggagag attgtactgg gtaaatatga
    17401 ggtattagat gacggtcaag atacagtcat actttgtatt ggaagtatgg taaaacatgc
    17461 tttagaggct aaagaaatat tatcaagaga aggaataaat ccgacaatag ttaatgctag
    17521 attcttaaag ccaattgatg aaggtatgtt aaaagcatta ttaaaaaatc ataaaaatgt
    17581 agttactata gaggataata ttgtaactgg aggatttgga agtagaataa ataagtttat
    17641 tatagataat gaatataacg taaatatact taatatagca ataccagaag aatttgtaaa
    17701 acatggcaat atagatgaat tatatgattt tgtaggatta tcgccaaaaa gtattgctga
    17761 taaaataagg aaattagtta ttgagtaaaa atctaatgta gatattgtaa attaaaattt
    17821 atataaaata tagaatttat caattagaat aaatatcaag tgtgttactt caagctactt
    17881 tagttatcat taaagtagct tttaacagaa aggtaattgt atgaaaaaaa gaatagattt
    17941 attattagta gaacaagggt actttgaaag tagagaaaga gccaaaaaag caataatggc
    18001 tggattggtt tttgttgata atcaaaggtg tgataaggct ggtacagaag taaaagaaga
    18061 ttgtagtata gaagtaaaag gaaatccaat accatatgtc agtagaggtg gtttaaagct
    18121 tgaaaaagca atgaaaaact ttgatttgac tattgatgga aaggtatgta tggatatagg
    18181 ggcatccaca ggtggtttta cagattgtat gctcaaaaat ggagcaataa aagtattttc
    18241 tatagatgtt ggttatggtc aacttgcatg gaaattgaga caagatgata gggttgtgtg
    18301 tatggaaaga accaatataa gaaatgtgac aatagaagat acaaagcagt ttgctgattt
    18361 tgcgtcaatt gatgtgtctt ttatatcgtt aaagttagta cttccaaagg caaaggagtt
    18421 agtacgtcat gatggggaag ttgtcgcttt aattaaacct caatttgaag caggaagaga
    18481 aaaagtaggt aaaaaaggag tagttagaga aaaatctaca catatagaag ttataaaaat
    18541 gatttctgat ttctcagtcg aaaatggatt tgagatttta gggttagatt tttctcctat
    18601 taaaggtcct gaaggtaata tagaatatct aattcatttg agaaatggaa atgaaggata
    18661 tgaatttgat ggtgagacat acaataacaa aatagtagaa gttgttgaag cttctcataa
    18721 tttagacaaa taggttaaaa gggggcttag atttgatcct cgaattatat atgaaaaatt
    18781 gtgccttagt tgaagaacta aggttgaata ttgataaaaa cttaaatata cttacaggtg
    18841 aaactggttc aggaaaatct ataataatag atgcacttgg actttgttta ggtgataaat
    18901 atgataggtc ttttttaaga aaaggaacag ataagggact tgttgaggct gtattttttt
    18961 cagacaatag atatcttaaa aaaatattag aagaaaatga tataagtatg gaagatgata
    19021 atttattggt aataactagg ttgatatact cagatggtaa gagtactgct agagtaaatg
    19081 gaaggactgt taaagtgtct cttcttaaag aaattgcatc tacacttata gatatacatg
    19141 gtcaacatca aaatcaagct ttgtttaata aagatactca tttaaaattt ctagaccttt
    19201 ttggagaaaa tgaattggaa gagtttaaaa ttgcttataa aaatgtgtac cataagtata
    19261 gtgaagtaaa aaaagcttta aattgcttga cagaaaataa agatgagatg caaattcaac
    19321 gagaaataga tttattaaga tttcaaataa atgaaattga agctgcaaat ctaaataaga
    19381 atgaatatga agatttatta aaacaaagag aagtttatag aaatagtgaa aagatatata
    19441 ataatctaaa ttcgagttat agcaagttac ataatggtga gtataatgtt attgacctaa
    19501 taggtcttgc atctaaggaa cttaatgaca tatctaaata tgacagtgtt ttaagtgaat
    19561 atagtgatac tgtagaaaga atcatgtatg agttacaaga catatctggt gagataagaa
    19621 attacaaaga taatatagac tttgaacctt atgagctaga gcagattgag cttagaatcg
    19681 acgagattaa taacttaaga agaaaatatg gagacagtat agatgatata tttgagtatt
    19741 atggaaaaat taaagataga ttagatgaaa tattaaacag agatgaaaga gttgaacagt
    19801 taagaagcaa actgatgaat atagaagaag atttaaaaat aaaagccagt aaattgacta
    19861 aagcaagaat agaagtagca actactttag aaaaaatact tcttgatgaa ttaaaaagct
    19921 taaatatgaa aaatgtaatg ttcaaggtta attttgaaaa aggtccattt actttagatg
    19981 gagtagatga tattgaattt atgatatctt ttaacttagg agaagatatt aagcctatat
    20041 ataaagttgc atcaggtggt gagatgtcca gatttatgtt agcgtttaaa accatattgg
    20101 ctgatataga tgatattgat acattagtgt ttgacgagat agatactgga ataagtggga
    20161 tagcagcaca gatagttggt gagaaattga gtgatatagc aaagaaaaaa cagataatat
    20221 gtataactca tttacctcaa attgctgcaa atgcagacac tcattattgt atagagaaag
    20281 atacttcaaa taataggacg tttaccaatg ttagtaagtt aaatgaatct caacgaaaaa
    20341 atgaaattgc cagacttata gcaggtaaca atataacaga aaaaactatt gaacatgcaa
    20401 gtgaaattat tgagttggct aaaaaatgtt agtgaaaaac aagcatttta ataggtgtaa
    20461 agaattttta tagaaatgct ttacatctat tttttatgat gaaattattt taaaatatct
    20521 agtgcctata ttgaagaaaa gtagatttaa aaatagaaga aaatttgtat aaatttgtaa
    20581 ctttgaaatt tctaaatgct taagtataat attatttatt aaggtagtag tgagggggac
    20641 ataaaatgaa taaaaaagat actatgcact taatttttac aattgtaatt tttattttat
    20701 tatggtatat tgtggtagca gtaagtgaga ttaataaaaa tattgaaagt tgtagtaata
    20761 aattagatta tttgtcacaa gagattatga gtactaaatc ggatattagt aatactataa
    20821 atgaagaaat gtcaaaaagt tatataacaa agagtattga cttaaaagta aaaaaaatcg
    20881 agaaaaaaga gtgtgttatt gatgtagatg ttcaattaag taaacttgga aaaaatggaa
    20941 aagtattttt tatgtataaa gaagatagtg ataaatggaa tgaaactgag atgacaaaac
    21001 atggagaatt atcatatgca tgtgaaatta agataaatac aggaagtgaa tatgactata
    21061 aagtagttac aagtggagct atatctgaaa gttctgatgt gcaaaaattg actaaatctg
    21121 attatatgcc tgaacaaccc atatttaatt ctgggataag agataataga gaatacttta
    21181 ttgaaatagt agaaaatagt aatcttttca atcatgagag tgaaaagtca aaaaataaag
    21241 tttatgataa tattagatta gaaaaagtag atattattgt aaatgaagga aaaaaagaca
    21301 ctatatacaa agccaaatta gctgaaggct tagatgatgg caaaactaat aattcaaata
    21361 gaaatcaagt gaattatgaa gtaagttttc ctaaaaggga tattgatgac ataatttata
    21421 ttaaagctaa acttacatac aataatggag tagaatatac aaaggatata actgaagata
    21481 taactatggg attacaagat ttagaaaaat aaatacatat ttaaataaat attcaaatag
    21541 atttgttcaa taaaatagag tataaataaa actgtagtag ttttacttat actctattaa
    21601 aaaattataa ttttaattcc atatatctag ccccttcaat tggattataa gtataaggtt
    21661 ctatatcata aaaaccaaac ttttcatata atccttgtgc acttttcatg cttggaagag
    21721 tatctaatct catgtgtgta tatccaaatt ttttagcttc ttcaatgatt tcttccaata
    21781 gtatttttcc tatttttaat cctctaaatt gatttcttac atataatctt tttaattcac
    21841 atattttacc ttcaagtttt tttaaagcaa cacaacctgc caaattttca tcaacaaatg
    21901 ccaatatcaa tgagcctgaa ggttttttat attttcctgg aagtgttttt aattcattat
    21961 taaagtcttg aaaacataag tctatattta gagaatttga atattcagta aatagaagtt
    22021 ttacgttttc aaggttttct aaatcttttt cttcaacaaa ttgtaaagtt atattttgag
    22081 ccattttatc cgccattata taaacatgtt tatataatga cttcacctcc ttgatatatg
    22141 tatatttttc ttaatatcaa ttagatttta ataccattac tttttcacca ccatagtcaa
    22201 gctctccatt aaactcaaat ccaaaatctt tataaagctt aatagccatt ttattgtctt
    22261 cgaatatact aagatatagt tcatcacagt tatattcatt tttaagatga tttacaagaa
    22321 aatctaatga tgcttttcca tatcctttac cttgatattt tgaatcaatc ataaatctat
    22381 caagccaaac tctatcatct tctaaacgac catacatagc aaatcctatt aaagaatttt
    22441 catcatatat tccaacaggt acccaagctg ttataaattt agattcagca atagaaagtg
    22501 cattgctttc tatataatct aactgacttt tatctacaga caaactaact aaatcatgcc
    22561 aattatttat gtctgcaatt cttacattta atttcaagat attaccgccc ttcaaacttt
    22621 ttgttacaat ctaatactat catattatgt attaaaaatc acttattaag attttaaata
    22681 aactttttaa atgaataaat tctcaggaaa aaggaattat aaatacataa taatacagca
    22741 taggagtaga tggatatatg aacaaaataa ataaaaattt caaaaatatt tggataatat
    22801 taggagctgt atcgattttt accatggtta taatttttaa taaaaattgt aatgttaata
    22861 aagagactca aatagaagct tttacaatta agaatcatga taaaaaattt gtatatccta
    22921 tgggaaatat aattggagta aaagcaaata cagatggtgt attggtctta ggatatgagg
    22981 aagaggatgt agactatatt ggaggcatac aaatcggtga taatatagta aaaatagata
    23041 ataaaaggat aaaaaatagt caagatgtat cagagatttt gaataagatt aaaaaaagta
    23101 aagttgaagt gacttttgag agaaaaaata agtataaaac agaagtaata gagactaaaa
    23161 aagaaaatgg caagtataaa cttggattat gggtaagaga taaaatatct ggaattggaa
    23221 ctatgacttt ttacgaccct agtatggaaa agttcaaagc aatcggtcat gccataaaag
    23281 attcggatac taatgaacta ttaaagataa agcaaggata tatatataag ccagaacaat
    23341 taaaaatagt aaaagctagt aatgaaaaag taggtaaaat aaaaggagat tttaatgaca
    23401 gcaatttaat gggtaatttc tcaaataatt cagagttagg tataagtggt aatattacag
    23461 aaaaccataa taaagagttt aatgtagcaa ataaagaaaa gcaattaata gaagttggaa
    23521 gaccgcaaga tgtaaaaata ggagatgcag taattctttt tgaggataaa aacaaaaata
    23581 taacaagcta tgatataaaa atagaaagta tagtatatga taaaggaaat tatagagata
    23641 tggtaataaa agtagtagat gacaagttat tggaatacac aggaggtatc gtacagggga
    23701 tgagtggagc tccaataata caaaataata aaattattgg tgcaataact catgttttta
    23761 gagataatcc gaaaaaagga tatggtattt ttatagatga aatgataaaa ttgtaggtga
    23821 ggcattaaaa aattttatta ttttatcaat tatctaggag gaatataatt ttggagtgtc
    23881 gaatatgctt tagagtagat aattaggaag caattgtgta aaaagtttag ttttctgtaa
    23941 taagaagatg ttttttaatg gggggatttt tagtggaaaa aatcaaaata gttttagcag
    24001 atgacaataa ggatttttgt caggtattaa aagagtattt gtctaatgaa gatgatatcg
    24061 atatattagg catagctaag gatggaattg aagcattaga cttagtaaaa aagacacaac
    24121 cggacttatt aatactagat gtaataatgc cacatctaga tggattaggt gtaattgaaa
    24181 aattgaatac tatggatata cctaaaatgc caaaaataat agtactatca gcagtaggtc
    24241 aagataagat aactcaaagc gcaataaatc taggagcaga ttactacata gtaaagccat
    24301 ttgattttgt tgtgtttata aacagaatta gagagctagt ttctaataga gttacacaag
    24361 ttgagccaaa acctagacca gttcaagaga ctcaaatgac tagaagtgat tttgttaaga
    24421 atgtaggaaa tatagagaca gaaatcacaa atataataca tgaaatagga gtaccagctc
    24481 atataaaagg atatttatat ttaagagaag caataaaaat ggttattgat aatgttgagc
    24541 ttttaggtgc agtaacaaaa gagttatatc caagtatagc taaaaaattc aatacaacac
    24601 caagtagagt tgaaagagca ataagacatg caatagaggt tgcatggagt agaggaaaag
    24661 ttgacacaat aaatcaatta tttggatata cggtacacaa tactaaagga aaaccaacta
    24721 attcagaatt tatagcaatg attgctgata aattaagact agaacatagt atggttaaat
    24781 aaacaagaca taaaaagtaa ggctttttaa ttaaggcatt ggctataaat gcgtattaca
    24841 agcagcgaaa cggtataacc actagggtta taccgtttcg ctattttaat aaatataaaa
    24901 attttcttta ttatttgctt actatatcaa tataataatt ttattatact atggatatag
    24961 tatgtgtctt tacaagttgt aaactgacag tggtttattt tttaatataa atattgactt
    25021 tgatgcaggt aaactttgta tttttaagcg tattatggaa tatgttaaat aaaaaaatga
    25081 tgaaatatag tattgtaaat gccaaagatg caaaacaaac ttaaaacatt tattttattg
    25141 ttaagtaatg ctataatata atgtgatttt aataatgata gtggaggttt aaatatgaga
    25201 gtcgaggccc ctataaaagt agatcgaaaa accaaaaaac ttgctaaaag agttgaaagt
    25261 ggggaaatag cagttataaa tcatatagac atagatgaag ttgctgcaaa ctctttagta
    25321 gaagctaaaa taaaacttgt cataaatgcg gctccttcta taagtggtag gtatcccaat
    25381 aaaggtccag ggatattaac tgaaaataat atactaataa ttgataatgt tggagaagag
    25441 ttatttgaag aattaaaaga aggagaaact atagaagttg tagatggaaa gatatataga
    25501 aaaggaaaat ttttaggtgc aggagaagtt ttagacaagt atgaggtttc acaccaaata
    25561 aaggctgcct atgagaattt agctgtagaa ttggatagat ttatagataa tactatagat
    25621 tatgctaaaa aagaaaaagg atttatactt ggagaagtag aaattccaaa agtgaaaact
    25681 aattatgcta ataaacatgt attgatagtt gtaagaggtc aggactacaa agaagattta
    25741 agtactatgc tttcttatat agaagaggta aagccagtat tggttggtgt tgatggagga
    25801 gcagacgctt taattgagtt tggatatact ccagatgtga ttgtaggtga tatggatagt
    25861 gtaagtgatg aagctttaaa aaaggctagt gaaatagtag tacatgctta tacagatgga
    25921 agagcaccag gtcttaaaag ggttgaggaa ttaggcttag atgcagttgt atttcctgca
    25981 ccaggaacta gtgaagatat agctatgctt atcgcttatg agtataaggc tgagttaata
    26041 gtagcagttg gtacacattc aaatatgatt gattttcttg aaaaaggaag aaaaggtatg
    26101 gcaagtactt ttttagttag actaaaaata ggttcaaagc ttatagatgc taaaggcgtt
    26161 aatttgttat acagaagtaa attaaaaata aagtacatat gggcattaat agcaacagca
    26221 ttatttcctg tgttggtagt tgcatcattg tcaccaggag ttcaacagtt catacaacta
    26281 atgcaattaa agtttagagt attattacag atgtaaattt taggaggata aatatgcata
    26341 ttaatatgaa atattatata gtcactatag gggctatatt tatagctttg ggtataggca
    26401 tgttagtagg atttaattta aataataatc aacaattaag tgaacaacaa gcaaatataa
    26461 taaatgattt ggatgataag tttgatatct taaaagaaaa aaatgataaa cttgacagtg
    26521 acttagcaag tgtaaataga gattatgaag aggcagttaa ttttataaat aaaaatgtag
    26581 ataagatttt agctggttcg ctgaatggta agagtatagg aataatatct gctaatgaaa
    26641 atgacgatta tacaaaaaat atagaagaca taataaataa atctaatgga agcattgcat
    26701 ttaatataat attaaaagaa aacataacaa atccagaaaa gttaaaggaa atgtcaacta
    26761 aactaggtac agatgtaaaa aatgcaaatg atgcagttaa ttatataatt gacactctta
    26821 aaaaggaaga tgcaagtgac attttaacat atcttcaaga actagatgta ataaagttta
    26881 attttattgg agatacatat ctaaaatatg attccgttgt aatagctggt ggaaatgatg
    26941 caaaagacag tgcaaaacag tttgaaaaaa tagaaaaatt tgtagtttca aaacttaaat
    27001 cagagaataa atatttagta gaagttcaaa atacaggagt taaaacttct tatgtagaac
    27061 tatattctaa aaacaaagtt gcaactatag acaatattga tgaaggtata ggaagtatat
    27121 ctctagcgat tcttctacaa caaggtaata tagttggtaa ttttggtaga ttggatactg
    27181 caacttcatt gttgccatct attaaataga aataatttta aggagagaaa aaatggattc
    27241 ttatataagt ataataatac cagcttataa tgaagaaagt agaataaaat atacattaga
    27301 aagcatattg gacattaagc aaataaatga aataatagtt gtggatgatg gttcaattga
    27361 caatactgct aaggttgtat ctgagctaga aaatgaaaag attaaatttt ttaagttaga
    27421 taaaaataga ggtaaaggat atgctttaaa ttatggtatc aaattagcaa tgaaaaatgc
    27481 agatattata ggttttctgg atggagattt aggaagttct gctaaagaag ttgaaaaatt
    27541 aatagtacct atattaaatg gtgaggcgga tgttacaata gccaagtttc ctcctgcaaa
    27601 gagaaaagga ggtttgggat ttgtaaaagg tttagctaaa aattctgtgt ttgagatgac
    27661 tggtgttgag ttagatgcaa ctttgtcagg tcaaagaata tttaaaagag aagttttaga
    27721 aaagtttgat gaaatacctt ttggatatgg agtagaagtt ggaatgacca ttgatatatt
    27781 aaagtatgga tttaaaatta aagaagtatt agtaaatatg actcataatg aaacaggaag
    27841 aaatttaaaa ggttttattc atagagggaa acagtattat cacataaaaa aggttttagg
    27901 acaaaaaaag aaagaatgga ggtaatatct tagtgaaaga ttatatactt tataccattt
    27961 tattattaac tggattattt ggaacctatg ctgtaattcc actatttaaa aatttattga
    28021 taaatggaaa tgtacttaga ccaaactata aaaaagatat gataccagta agtatgggaa
    28081 ttgtattttt gcctatgatt gtaatcaatg gaataatact tgctttcttt actacagaat
    28141 ttaaggattt agcgtatata tttatgttta tgtttggtat gatatccatg ttttttgcag
    28201 ggatactaga tgatgtcatt ggaaatagag atgtaagtgg tttaaagggg cattttaaaa
    28261 gtttatttaa aggaagtctt acaacaggag gttttaaagc tttatttggt ggatttgtag
    28321 ggcttatagt ttctgttgct atatcaaaag acataattga tataatagta aatacactaa
    28381 taatagctct atcaactaat ttaatgaatc ttttggattt aagaccagga agggcaataa
    28441 aagcatattt attcataatg gtaattatat ttttaacatt gacaggattt gttcaagttc
    28501 ttccattact tatagtacca aatgtacttg catactttaa ttatgattta aaagcaaggg
    28561 ctatgatggg agatactggt tcaaacgttc ttggaatatc tattggaatg ctcatttctt
    28621 ttgggtatcc atttaacatt agattaggat ggttaatatt tgtagttatt atgcatatat
    28681 tcactgagaa atactcatta actaagataa tagaaaaaaa taaactatta aattttatag
    28741 ataaattggg aaggtagtat tatgataagc aaaagagtgg ggattgttga atcaatagta
    28801 agtcaaactg aaactctaga tgatataaga gtaaatataa atggagaaat acaaaaggca
    28861 tataattatc caaaaatatc tggaactata aatataggtg acgaagtggt tttaaataca
    28921 acagcagtag aactgagctt aggtacaggt ggataccatt ttgtaataac taatttaaat
    28981 aatatagaat ctactttaac tgaaggtgga catataatga aacttagata tacgcctctt
    29041 caaataaaag tggattctgt tgaggaacaa gaaagtattt atcatgataa atttgcgaat
    29101 tttaaaggat tagaaggtct ttctgttgta gtagggactc ttcatagtat gttaactcca
    29161 tttgcagctt catttaaaag aaataatcca aataaaaaat tagtgtatat aatgactgac
    29221 ggagcatctc ttccaattta tttaagtaaa aatgtggata ctttaaaaga aaaaaaatta
    29281 atagatagta caataactat aggaaatgcg tttggtggag attatgaatg tataaatata
    29341 tatactgcat taattacagc aaaagaaata ctaaaagcgg atgcagtatt tgtcagtatg
    29401 ggacctggaa ttgctgggac aggaacaaaa tatggattta cagggattga acaaggctct
    29461 attcttgatg ccgtaaaaaa acttgaagga agagcaattg caattccaag aattagtttt
    29521 gctgacaaac gagaaagaca tcaaggtatt tcacatcata gtatgactgt atttaaagaa
    29581 attgtaaatg taaatgtaga cataccaata actatatatg atgatgaaaa attgaacttt
    29641 ataaaagagc aaatcaattt aaatggttta gacaaaaagc ataatataat ttatatagat
    29701 aataataaaa cgaaagagga tttagactat tttaatctaa aagtaaagag tatgggaaga
    29761 aattatgaac aagatgaggc atttttcaaa gctgcaagca cagcagcata ttacttaatg
    29821 gaggtttaag atggtattag aagaaaaaac tattagtagt gatagggttt atacaggtaa
    29881 agtaattact ttaaaggttg atactgttga aattccaggg caaggatatc aaaaaaggga
    29941 gttagtagaa gttggtggag cagtaggaat agttgcaatc acagatgata ataaagttgt
    30001 gttagttaag caatttagaa aacctataga aaagccaatc tttgaaattc cagctggtaa
    30061 actagaaaaa aatgaaagtc caaaagaatg tgctgaaaga gaattaaaag aagaaactgg
    30121 gtatagtgca aaaaatatta agctaataca taaatttttc acttcagcag gtttttctaa
    30181 tgagataatg tttgtatatt tagcaacagg tcttactcca ggagagaata atttagatgc
    30241 agatgagttt ttagatgtct atgaaattga gcttgaagaa gcatataaca tggtattaaa
    30301 aaatgacgtt gaagatgcaa aaacatctat tggattatta ttagtaaaag atatgtttaa
    30361 aaattaatat ttataaaatg aataaccctt tgtcctaaag catattattt tataaaggac
    30421 aagggggaat taaattgaga agaacatata gaaaaaatga ttttagagaa ttaaatagac
    30481 aaataattat tataggattg ttgtttatga tgtctattgt tgttgggtct tacataaata
    30541 aaatattgcc aggttctagt aacaatatat taaacaatat aaatcctgct gtagagtatt
    30601 ataatctaaa tataagcata aaagatacag tgatacaaaa tttaaaatca gatgcaatct
    30661 ttatgggctc aatagctcta ttaagtctat ttgtagtaac aattccagca gtgttggtag
    30721 cttttgtatt aaaagggatg tcaattggtt atacaataaa tagttgtata ttggcattaa
    30781 aatttaaaag tataaaaatg attttaataa tattatttaa aaatttgatt attattccat
    30841 gtgcaatcat attagcatta attagtttaa gttattttaa agaaatggtt tatgaattta
    30901 aaaagaaaaa tagaaaaaat atgcaatttt taattaagag gtatatacta aatattataa
    30961 ttataatagc actatcattg gggttgcaat taatattaaa tactgcaagt attggtatta
    31021 ttaagttcct agctaaataa aggagtttat aatggatatt atagagggat atatagatta
    31081 cataaaagat aaaaaaaaat tatcagagaa tacagtggca tcatacttta tggatataaa
    31141 aaaatatatg gaatatataa atcaaaaaag cattaaacta gtagatatag tagaaaatga
    31201 tattataagt tacttaataa agttggaaaa agataatgta tcaatagcta caatagcgag
    31261 gatgatatcc tctataaaat cttttcatga ttatttattt ttaaatcata tatgtacaaa
    31321 taatcctgca aaggatataa aaaaacctaa aataaaaaaa gaaaatataa atatacttac
    31381 agaagaagaa attgaaagac tattaaattt tccaaagtta acaacaccta aattgattag
    31441 ggacaaggct atttttgaag tattatatgg tactggtata aaagtatctg aattagtaga
    31501 gatgaatata gaggatattg acctagatat tgattatata tattgtaatt catgttgtaa
    31561 aaatcaaaga gtgataccac tttgtgatat tacaaaactt tacttagaaa agtatctaaa
    31621 agaagcaaga cctaaaatgg ctgtagaagg tgaaaaatca ttattcgtaa gttcattagg
    31681 tcaaaggttt acacgacaag gtctttggaa agtcataaaa aaatattcta atctagccaa
    31741 tatagataaa aatataaatc ctacaatgct tagacattca tttgctattc atttgttaaa
    31801 tgaaggtgct aacattgctg ttgtaagtaa aattttagga aatgttaatt tatctagttt
    31861 gcaagtatat ttaaatcata tagataaaaa cgtgagaaga gaaataaagg agaaacatcc
    31921 tagaaatgat gttgatgtag aattacaaaa agcagaagct aaatagacta tatagcaaat
    31981 tttataattt agaaggagca tatttatatg agcagagtaa tttggattgt aattgatagt
    32041 gttgggatag gagcattgcc tgatgctgaa aaatttggtg atagtaagga tgtaagtacc
    32101 cttggaaata tatttaagga atatccagat attcagattc ctaatatgag aaatttaggt
    32161 ataggaaaca tagatggtat agattttttt gaaagtataa aagaaccaat aggttgtttt
    32221 ggaaagtgta aggaaatgtc tcagggaaaa gatactacta caggtcattg ggaaatgact
    32281 ggtataatag ttgacaaacc atttaagaca tttgaacatg gattctctaa ggagataata
    32341 gaagagtttg agaaaaaaac tggaagaaaa gtagtaggaa ataaaccagc ttcaggaact
    32401 gtaataatag atgagtatgg agaacatcaa ataaaaactg gagatgtaat agtatataca
    32461 tcagctgata gtgtatttca gattgctgct aatgaagaag taattcccct agaagaactc
    32521 tataatatgt gcaaaatcgc tagggaaata atgatggggg ataatgcagt agctagagta
    32581 attgctagac cattcatagg taagaaaaaa ggtgagtttg tgaggacttc aaatagaaga
    32641 gattattcac tagacccatt tgaaccaaca gttcttgata atataaaaga atcaggactt
    32701 gatgttttag cagttggaaa aatagaagat atttttaatg gaaaaggaat tacagatgct
    32761 atccacacta aaagcaatat ggatggagtg gatgaaactt taaattatat gaaacaagat
    32821 aataaaggtc taatatattc aaatctcgtt gattttgatt ctaaatatgg tcataggaga
    32881 gacccagaag gatataagaa agctcttgaa gagtttgata gtagacttcc tgaaataatg
    32941 gctaatatga gagaagatga tattttaata atcaatgcag accatggaaa tgacccaaca
    33001 tataaaggta cagaccatac aagagaatat atacctgtta tgatttatgg caataagatt
    33061 aaaaaaggtt ttaatttagg agtaaaagat acttttgctg atattggagc aactgttgca
    33121 gatatactaa atgtaaagtt gcctaaacat gggtcaagct ttaaagaaga tttattttaa
    33181 actataaaat gataaaaact tagcttaata ggatggagag attggaatgt ttgaaaagat
    33241 agcacaaagt agtaaattta taaattctaa aagtgatata aaacctaaga ttggtttgat
    33301 attaggttct ggactaggtg atttagcaaa tgatatagaa gaccctgtcg caattaaata
    33361 tagtgaaatt ccaaacttcc ctgtatcaac agtagcagga catgctggac aacttgtaat
    33421 tggaaaactt gaaggaaaag aagttattgc tatgcaaggt agatttcatt actatgaagg
    33481 atattctcag aaagaagcta cttttccagt aagggttatg aaagaattag gagtcgagat
    33541 tcttatagta actaacgctg ctggaggcgt aaataaagaa tttaaagcag gagatttgat
    33601 gcttataaga gaccatataa actttagtgg aagtaatcca ttagtaggaa aaaatgatga
    33661 cagatttggt gctagatttc cagatatgtc agatgcatat tcttctaaat atgtagatgt
    33721 agttaaaaac tgcgctaaag aatgcaatat agatgtcaaa gaaggcgttt atatgttttt
    33781 tagtggtcca aactatgaaa caccagcaga agtgagaatg gcacaaattt tgggagctga
    33841 tgctgtaggt atgtctactg ttccagaagt catagtagct tctcactcta atataggagt
    33901 tattggtata tcttgtataa caaatatggc tgctggcatc ttaaatcaac cactaagtca
    33961 tgaggaagta atagaaacta ctcaaaaagt taaatcagaa tttatgaatt tagttaaaac
    34021 aacggttaaa aatttataag ttaaagctta ttatagattt tttgaggaag tttataataa
    34081 aatagttaat gtttaatttg aaaagaatta ataaaaagct gtcttgatat agaaagttat
    34141 ttctaataaa ggacagtttt tttataatac tacaacattt gaaagtgttt taaagtttaa
    34201 tatttattta aattattata ggttagaatg gagatttcaa ttatgagaat gtatgatatt
    34261 ataaaaaaaa agagagataa cctagagctt agtaaaaaag agataaacta ctttatagaa
    34321 aactattcaa agggagatat acctgactat caagcttctg cactattaat gtcgatttat
    34381 ttaaataaga tgaataaaca ggaaactgta catcttacag aagcaatgat gaactctgga
    34441 gacatgataa acctatctga gatagatgga ataaaggtag ataagcatag tactggtggt
    34501 gttggagata agactaccat agctcttata cctttggttg cttcatgtgg agcaccagtt
    34561 gcaaagatgt caggtagagg tcttggtcat actggtggta ctattgataa attggagtct
    34621 ataccaggtt tttcgacaga aatggagata agtaaattta ttgattctgt aaataagtct
    34681 aaaatagcag tttgtggtca aagtgcaaag gtagcaattg cagataagaa aatatatgct
    34741 cttagagatg ttacagcaac tgttgataat atatcattga tatcatcaag tgtaatgtct
    34801 aagaagttag cttcaggagc tgatgcaatt gtgctagatg taaaaactgg tgatggagca
    34861 tttatgaaga cattagaaga gtcttttgag ttagctaaat ctatggttga tataggtact
    34921 ggtatgaata gagatacaat agggattgta acagatatgg atgaaccttt aggttttgca
    34981 gtgggaaact ctttagaagt tatagaagca attgagacat taaaaggaaa tggacctaaa
    35041 gaccttgttg agctatgcga agtattagga gcatatatgt tggttttatc aaaggtagca
    35101 catgattttg aagatggtgt tgaaaaaata agagaatcaa taaaatctgg tactgctata
    35161 caaaaactca aagagtttat agagaatcaa ggtggtaata aagatgtagt agatgattat
    35221 tctttattca aaaaatcaaa gtataaatat ccagttaaat caactaaatc aggttatata
    35281 agcaaaatta aagctgaaga tgttggtgta tcggctatga ttcttggagc aggtagagaa
    35341 actaaagatg atattttaga tttatcagca gggataattt tagaaaagaa agtaggagat
    35401 tatgtaaatg aaggtgaagt tttagcttac atgtattata atgatgaaca aaagctttca
    35461 ttggcaaagg gaagatttat tgattcctac tctattgtag attcaaaaat agaaaaaaat
    35521 aaattaatat atggtattgt aactaaagaa gagattaaaa agttttagaa aattttagaa
    35581 aagtgattat aaaaggccta acatgtcaaa aataatgtta ggttttttat tttttgatag
    35641 actataaatt ataccaaaaa tatatatttt aaaacatttt ttacaatata aataagttta
    35701 ttctacaaga tgattgaaaa ttaaagtatc tagttttata atagaaaatg gataaatata
    35761 cataaggagt agattatatt atgaacttta aagaaaatag attgaaaata gcagtattaa
    35821 taattgttat tttgattatc ttagcaggta tatttgtgtt tatacaaata ggtccttatg
    35881 ataaaaataa caaaaaagat gtaattatcg acgttccaag tggagcatct gttggaaaaa
    35941 tatctgacat attatatgaa aataaattaa taaagaatga actattattt aaacttttgg
    36001 taaaggttag caataaagct ccgtctataa aatcaggaac atacttactt aatcaatcgt
    36061 attcaaacaa tgatataata agtcttttag tttcaggtaa aatatatcag gatggtataa
    36121 aagtgactat tccagaggga gctacatcta aagaaattat agcaatgtta gtaagtaaaa
    36181 atttaggaga taaagctact tttgaaaatt taataaaaaa acctcaagaa ttttatgata
    36241 aatttccata tttgaaggaa gatggaataa cctctttaga agggttcttg tatccagaaa
    36301 cttattattt taattcaaag aaacaatcag aagaagatat tttaagcgaa atgttaaaag
    36361 tatttgacag taaatacacg gacaagttta aaaagaaaca aaaagaatta aatatgactc
    36421 ttcaagaagt aatggaaatg gcatcaataa ttgagaaaga agctgttctt gacaaagaca
    36481 gacctataat agcaagtgtg ttttataata gacttaaggt aggtatgcct cttcaatcag
    36541 atgctacaat acaatatata tttgaagaga gaaagaaaat tgtaacatat gatgatttaa
    36601 aaattgattc accatataat agttataaaa ataagggatt acctcctaca ccaatatcaa
    36661 atcctggtat aaaatctatt gaagcagcat tatatccaga gaaaacagac tatttatatt
    36721 ttgtagctaa gattgatggt ggaaataatt atagtacaaa ctatcaagac catttaaaat
    36781 acgttaaaga atataaagaa gcaagggata aacaatcaaa agatacaaaa gcaacaaata
    36841 aagaaaatac aaaaaaataa accatgatta taaaaagtct aacagggtaa gaataatgtt
    36901 gggctttttt attgaaaatt gttattctta gatttataat aaagaaagtg taaatatata
    36961 taacaatatg aatttaaaaa ataataaatt aatataaaac ttgaatattt attattttag
    37021 ggagtgtttt tatgagtaat atagttaatg atttggttga agattatata agagtgacat
    37081 taaaagaaaa agaaggattt ttaaaagatt tagaaattta tgcagaggaa aatagtgttc
    37141 ctataataca taaagaagtt tcagatttat taaaagtctt gttaaaggtt caaaaaccaa
    37201 agagaatttt agaagtagga tgtgcaattg gttattcttc tatttttttt gcaactatta
    37261 taggaaaaga tgcagatata ataactgttg agagaaatga aaaaatgata gaaaaagcaa
    37321 aagaaaacat aaaattagct ggatttgaca ataacataac tatactagaa ggagatgcag
    37381 aagaaaaact tagtcaagtc caaggtgaat ttgaccttat atttatagat gcagccaaag
    37441 gtcaatataa gctatttttt gatttagtta tagataaatt aaaagatggg ggacttttag
    37501 tatctgataa tattttgtat aaaggtatgg ttgctcatga tgattttgtt gtaagaagaa
    37561 aaaagactat agttaaaaga atgagaaatt atttagatta tatatgtaat tgtgactatt
    37621 taagcacatc attggtacca ataggtgatg gtgtagcact aagttataaa gaaagtaaaa
    37681 gaggtgatgt agatggaatt aaataaagta gaacttttag ctccagctgg tgatttggag
    37741 agattgaaaa tagctataac atatggagca gatgccgttt atataggtgg agaaattttt
    37801 ggaatgaggt ctgcagcgaa gaattttagt aaggaagata tggcagaggg agtagctttt
    37861 gcacatgaaa gaggcaagaa agtattcgta acagtaaata taatacctca taatgaagat
    37921 tttctacaat tagaagacta tttgttggaa ctagaagaga taggaataga tgcagttata
    37981 atagctgacc caggagtgct tagtgtcatt aaaaaagtaa ttccgaatat ggaaatacat
    38041 ttaagtacac aagcaaatac aaccaattat ctaagtgcaa acttctggta cgagcatgga
    38101 ataaaaagag ttgtagtggc aagagaatta tcttttgatg aaatatctga gataagagca
    38161 aaaacacctt tagatatgga tatagaagcg tttatgcatg gtgctatgtg tatttcttat
    38221 tctggcagat gtttaataag caactatatg actggaagag atgcaaacaa aggttcttgt
    38281 gctcaatctt gtagatggca atatcatttg gtggaagaaa aaagacctgg tgagtatttt
    38341 ccaatatacg aagatgaaag gggtacattc ttctttaact caaaagattt atgtatgata
    38401 gagtatatac cagagttaat caaatctggt ataacaagtt taaaaataga aggtagaatg
    38461 aagactgcat actatgtagc tactgttgta agagcataca gaatggcaat agatgaattt
    38521 tacagagacc cagaaaactg gaagttcaat ccaatgtgga tggaagaact aaaaaaaggt
    38581 agtcatagac attttacatc aggtttttac ttgaataaac caacaacaga agaccaaaat
    38641 tatcaatcag catcatatgt tagaaactat gattttatag gtatagttag agaaacagaa
    38701 gatgaagatg gtcttattgt tgttgagcaa agaaataaaa tgtgtgttgg tgatgaaata
    38761 gaagttatgg gaccatataa agaaactatg tttactaaaa ttgaagaaat gtacaatgaa
    38821 gaaggagaag ctatagaatc agctcctcat cctagacaaa tagtgaagtt aaagctatca
    38881 gtaaaagttg gtaaagatta tatgcttaga aaagttatag aagaaaaggt tgaagaataa
    38941 taaaaaattg ataaaaatag tattagcccc ttagaaattg tcaataatga aattatctaa
    39001 ggggtgattt catgtcaaaa aagaagactc catttttgaa aaaagtggga aagagaagct
    39061 ggtgtatatt tactataatt ttaataattt atagtgtttt aatttatagg ctagttgata
    39121 tacaagtatt aaaaggtgat aaatacaaac aaagtgttga atcacaaagc gttgagaaag
    39181 tcgagttaaa tagtggaaga ggaataatct atgatagaaa caacaagaag cttacagata
    39241 caagtaaatc tcaggtatta attgttgaaa aagaaaaatt aaataataac tataaaattt
    39301 tggagcttat taagaaagct actaagatga atgatttaga tatatataaa gctgtacaag
    39361 agcaattgac acgccctata atacagattc agactaaaaa tatcgataag agtatgaaaa
    39421 aagaacttga gaaaaatgga ataatggtag aagaaaaaac tatgaggtac gcaaaagatg
    39481 gtttattatc acatacaata ggatatataa aagaagatga taaatcaggt caatcaggaa
    39541 ttgaaaagag tatggatagt gtacttagaa attcaaatga aaagtatata agtgctttta
    39601 aagctggaga tgcaggaaat gaaaaatctc taaacatact aaagggaagt gtaaaaactg
    39661 ttgataataa ggataaagat agacatctaa aaactactat tgattataac attcaaaaaa
    39721 aattagaaca aatactaaat aaagaagaaa atcctacagc agcaataatc tcggaagcat
    39781 ctacaggtga aattcttgca atgtgctcta gacctaattt tgaccaaaat gacatatcaa
    39841 aaagtttaaa aggaaaaaat ggagaatttg aaaatagagt tataaaagcg acatatccac
    39901 cagggtctgt atttaagatg gtagttttat tttctgcact tgaaaatggt gttatagatg
    39961 agaactatac ttacaactgt acaggtaaga ctaaagttgg gaatactaat gaaatactgc
    40021 gatgtaataa aagagatggt catggtttcc aaaacttaag acaagctttt tctaattcat
    40081 gcaatcctgc atttttagat atagctatga aacttggaaa agaaaagata ctcaaatctg
    40141 ctgaaaaatt acatttattt gaaaaggttg atattggtct tgatgaagaa aaaattagag
    40201 aagctccaaa aaacatatca ataagaaatt tagctatagg tcaagaaaat atagaattta
    40261 caccacttca aataaatcaa atgactcaaa taatagctaa taatggaaca ttcaaaccac
    40321 tatacttata taaaagtctt gtggacaata atatgaatac aataaaaact tataagtctt
    40381 caaaaaaaga agaacttatt tctccttatg tgtgtacaca agttaaagaa tatatgaagt
    40441 ctgtatctag gattggtaca gctaaagacc taaaagatat tgaaggtggt tgtggagtta
    40501 aaactggtac agctcaaagt agtttaaata aaaaagctat agaccatgga tggataacag
    40561 gattttatcc tgaagaaaga cctaagtatg taataactgt gttagtagaa ggaactcaaa
    40621 aaggaaataa atctgcaaca cctatattta aggaaatatg tgaaagtata aaataaatta
    40681 tgtactataa aatttaagga tatatcgtaa ctttgatttt agatagaaaa ttttgtctaa
    40741 tgatgtacga tatatccttt taaaatatag tattaacttt cttgaacaag ctctttttct
    40801 agttttttaa gagctttttt ttcgattcta gatacatatg accttgatat atcgagtttt
    40861 tgtgcaattt ctctttgtgt cttatagcca taaggagtta acccatatct taactggaca
    40921 atttctcttt ctctaggagt aagaattttg tttagttgtt cgtaaagttt tccaacttga
    40981 acttttagtt caacttcatc aagaacatag ttagcctctg taccaagtat atctaaaaga
    41041 cttatttcat tacacactac tctttatatg ttattggtat tactggtctt catgtaattt
    41101 caaatggacc attaattcaa aagaatctag tttttgttct ttagacttat aataaactgc
    41161 tttatcaatt acagatttta ggagtttatt ttttccaagg gaatctgaat catggtaaag
    41221 tgataacaca gttttaatat cttctattat tttagaattg tcttttttaa cagtacttaa
    41281 tctatttttt actgtcttaa ttgaatcttt aatttcttca gttcttgaac ttatattgtt
    41341 ggacctttca ataaatgtat cttcatcata tattcctcgt tctagtaaat catataactt
    41401 atttttttgc ttgcctaatt cttttaattc agtttctaag ccctttaaga ctcgtttttg
    41461 aaattctata ttactatctt tattattttt atctaagtct atatcagatg cttttaattg
    41521 atattttttt acagtgtctt ctaaagattt taaaatggca gcttcaataa attcaaatcg
    41581 agaactttta ttgcaaccag gatgataaca aattatatag tcgtggtctg catatggtct
    41641 atatactaat ggtctaccac aatttttgca aatcactatt cctgcaagtg gattttttat
    41701 ttgattacca tagctgacat gaccattttt tttgagtata ttttgagctt ttttccatgt
    41761 tatttcactt attaaaggtt catgctttcc ttttgcctca atccattcat ctttaggtct
    41821 tagtttaact gtttttattt tatttggatt tttagatttt ttgtagtcct ttttttgcca
    41881 ctgaatgtag ccacaatata ccttattttt tattatattt gtaattgcag aattgtacca
    41941 attagcacct gtagcagttt taatgcctaa gttatttagg tattttgata ttttactaca
    42001 acccatatct tcattaatat ataagtcaaa gatagtccta actacatcag catccttatt
    42061 tggtacaagt attctttctt ttctaccagt tgttacagca tcatatccaa atggagcaga
    42121 ggttcctatg aaattccctt cttcaactga ttttattcta cctctttgca tccttcttga
    42181 tataagtttt aattcttttc ttgccataaa tgcttcaaat tctgaatact cctcatcaaa
    42241 ttcatctgtt aggtcgtatg tttttctagg tgtgataatt ttggtcttac tttctttgaa
    42301 tgtttctaat atgatacctt ggtctttcat gtctccacga ccaagtctgt ctaagtccat
    42361 tactaagact gcatcatatt tattttcttc aacttctttt agaagttcaa gcattttggg
    42421 tctatgtatt atactttcac cagaaactaa ttcttctttt atctcaatta catctaagtt
    42481 ttgttctttt gctagtttta gtagggttga tttatgtcta cttaaagttt caaattcgcc
    42541 ttgtttttct gcctcttcat cagcacggga ttttcttaag tatatggcaa cactcataat
    42601 aagtaatcac actccttatg cactattata tagtaaatca atagagttta aaatatagaa
    42661 aataagcatg tcaaaagtta atttaacttt caacatactt attttctata atattaatgc
    42721 cttataatcc tttaatatca tttagattag tttttgcata ttcattatcc gggttaagtt
    42781 caagcgcctt attgtaagat tctctagctt tattaaattc tgatttactc ataaaaataa
    42841 cacctcttaa attgtaagca tcactagcgt ctattttata agcttcatta tttttatcta
    42901 gatattctaa gtatttatca attgacttta aagcctcatc atatcgttgt aactctatat
    42961 aaatatatga ttgtgtataa tatgcaagtg gatttttatc atcaatttct atagcttttt
    43021 tgatatcaat agcagcttta ttataatcct tatcttcatc tttttctgtt tctgacctag
    43081 cgattttgac ataattagag ctaagagctc ttttaaagtc tttatgccat aataatttat
    43141 attcattatc ttcgattata aaatatctat caaaatttgc agtatcttct ttattttcag
    43201 tgatatactt tatagtatca gtttctttta ttttaacaag atttgtgtac tctttattag
    43261 aatatttgta tttctctact ttattttctt caccaagttt ataatctttt agggtttgaa
    43321 gtttaaataa agtatcttga tattcttcaa atttttctag actgacgctt tccttactgt
    43381 ctttacttaa taaattatat gctacttcgt agtctttgat ttttatacta tcaaggtact
    43441 ttttagctac ttctgtagca ctttcttttg gagcacatcc aaccattgat atcataatta
    43501 aaaatgatac taatattgct gttatttttt tactgattat cataatataa tcccccttat
    43561 attgtctctc attaatttta ttttatcacc atattctacc aaagtcatta atttaggagt
    43621 gatatttttc attaatatta tattaagtaa taataacttt ttaaatttat ggtaacattt
    43681 tcctgaaaag tattatataa tttaggtgaa aatatttaag agaaggagat attatgaaaa
    43741 ttaaaaaatt attaacaata gggttatctt tatctatatt tattgctagt tgccctatat
    43801 ctgcaaatgc tcttgataaa attgaatcaa ttaaaggtgc agataaatac gaaactgctg
    43861 gtataattgc agacaaacaa aactatacta cagctatatt aattaatgca gacagtacaa
    43921 tgtctgatgg attaagtgca agtggacttg caggagctat aaacgctcca attcttttga
    43981 ctaagaaaaa taacatacca aatgctacac ttaaaagatt agaaaaggca aaaaaagtat
    44041 atataattgg aggagaaaac tctattgata aatatactga aactgttcta aaaggcaaag
    44101 gaatagagat taaaagactt caaggtagtg atagaattaa gacaagctat aatgtcgcaa
    44161 aagaaataaa ctcaattaac aaagtaaata aagtcatatt gactaatgca tttaaaggag
    44221 agcctgatgc aatgagtgtt gcacctgtag cagttagaga taaagcagct atagtattaa
    44281 ctgatggaaa aagtgttcca tttaatacaa ctggtgtaga aagttatgta ataggtggta
    44341 cttcatcaat gaatgacaaa atagttaagg atactaattc aactagatta ggtgggacag
    44401 atagatatga tactaacaaa aagataataa ataaatttta tagtggagta aaagaatttt
    44461 atatagcaag tggtactgat ttagtgtatg ctcttgttgg ttctactgct gctaaaaata
    44521 atgctattgt gttagttgat aatgatagta ataaaagtgt attgaaaagt acaactaaat
    44581 taactgctat aggaaatttg agtgatagta tattacagca gtgtttaaat gtaacaaaaa
    44641 atataggcga ttctaatact ggaagagaat atacagtaga agatgctgct aatgcagtga
    44701 aaaaactatt aggtgtgaag aaattttatt acaaacctaa ttattcagaa gatgaagatt
    44761 taatatttct actagataaa aattttgatt tttcaggaaa agagtactat gtatttgctt
    44821 acattaaaca gggtgcagag ggtgatgtta gatactgtgt agctaaaaat gatatgagca
    44881 aagtttatac atatgatgtt aatggtaata tgaaagtatg taatccagat gattataaaa
    44941 ataaaggaag tataacagag gaacaagcaa aacagatagc attgaaagaa tgtgctaaga
    45001 gacataaagt cagtatcaat aacttagtag ttgatatgat agattttaga gatgatatat
    45061 atagtggaaa tgtctatgct gttaaaatca attcaaaaaa tagtaatggt acttttggtt
    45121 ggttttttat agatatagaa actgggaaag tttataatta aaattagaac ttttaaacaa
    45181 gtgtttttaa ttatataatt taccataata tgttataata tttttagcaa gaagatttaa
    45241 cttacaatct atagagtgga gttcatgtac aaaagattat cctcccaacg attcgaaggg
    45301 aggtgagttc atggataact ttttgtttaa tgttttagct agtttaacag ctagtgtggt
    45361 agtttactta atcagtaaac tattcaaaaa agcaaaaagc cactctcgca caaagagtga
    45421 cttacgagtt gaatttaaat ttatatttaa atccaaaaaa taaactctgt ttatgatgaa
    45481 ctccactcta acgcaaaata gattgtagtt cttcttgctt ttattatacc acaaaacatt
    45541 ataaataaaa ctgttgttta taaaaaaatg caaagtaaaa ttttttataa acaatgataa
    45601 aaattttatt atagagctat taacttatta aatatctgta aatgagcaga tatttcataa
    45661 gttaatattt attgtttaca taaaatattt ggtaaaaata agtaagtttt atatgttata
    45721 atagttgtag caagaataat aatcgaaagc agcaagcatg tcagctggtg ttatatcagt
    45781 ttatatttat gataaaataa aaatctccca aacgccaata agaatgattc aaaaaaataa
    45841 tattttcact taaaaataat cactctttat aggagtaaat tattttcttg attttattat
    45901 actacaaatt ggtacagata tttaaaaata atatattcat aatattataa atagttttgc
    45961 agtgagcgat atttttgata aaatagggct taacagttga aacataaggc attgagggta
    46021 tatgataaat attatcattt gcactactcg tggttcactg caaatttgag agaattgtat
    46081 agatgtaagt cttggaaatg ctcaatttat tttggggttt tatattaact aagtggtatg
    46141 taaagtgttc ccctctaagc tctgtatata tatattccgt ttgttttata ttaactaagt
    46201 ggtatgtaaa gaagtgttga tatttatatt gtctatcccc gctagcttgt tttatattaa
    46261 ctaagtggta tgtaaagtcg taaattacga ccgtaccttt cgtaaaataa caatcgtttt
    46321 atattaacta agtggtatgt aaagcttttt gagtatgagt acaagcatag gaaaattaat
    46381 agttttatat taactaagtg gtatgtaaag aacaaaaaag atacatctgt aactaatttt
    46441 tatactgttt tatatcaatt aagtggtatg taaagacaga tataatatca aaataaggtt
    46501 aggactttta aaagcatagc catatcagta aaaatttaat tatcatttaa aaaataaatt
    46561 tttatttaaa gaatacacat aaatatttcc agactcaact ctcttaaact attttcatat
    46621 cttttagtag atacctttgt tgttttttaa tctttagcaa cttgttcttg agtgaacctc
    46681 ttattttttc taagttattt caagcttttc aaaaaattct ctatttatat tcatcacaca
    46741 tataatacaa ttaattcagc tttaattgcc aatatttagt ttttctgtat ctgataaacc
    46801 aagaatataa tcagtatata aaccaaaaat tttagcaaat attattaacc catcgtctct
    46861 tgttggtctt tcaccagact ctattctatt cataacactt gtatttatat ttgttttttc
    46921 aagcaattct ttttgagaac tattcatatt ttccctaata tatttaatcc tttgatttac
    46981 atccatacat aattatctcg aaaagtttct gttttggaaa tattgttatt gaaatatagc
    47041 aattaatgtg aatatatgaa aaagatacac tatgatttta taaaaaagaa gacatacata
    47101 aaatttatac ttacaaagtg ttgcaaatga gttaggtttt aaaaatgcat caatttattt
    47161 taagtacagg aaaggggaat attcaattag agttgatatg ttacctataa actatctaag
    47221 atacttgatt atgatataga aaattttttt acaaattaaa tttctaagat agaaatttaa
    47281 ttaagaggat gatgaggatg aataatttac aagtaatata aagaaataat aagagaattt
    47341 taaatgcata gcaactagca gatatatatg aaaatgatgt aaacaatata taaactaact
    47401 tcaatagaaa taaagagaat gtaaattatg ttttattaca attcgagtat ttgaaagaat
    47461 ttaagaacta atctattaat agtcagttga tttcaaaaca ttctagtcaa ttataccttt
    47521 ggatcgaaag atgtgcaaat agacactgca aaatattaga tattgataaa acttgggaac
    47581 aatttgacaa tttagagaaa atatatttca agattaagcg agagaaacta agctgtatag
    47641 aagatgtatt aatagaaaat ttaagattta aggatacaaa ttaataaagc aataatatgg
    47701 cattagatgt aaaaatagaa gttaaaataa taaaagatgt tgttatatta aatataacac
    47761 attggaaaaa agaaacaagt ctattaacag ttaaaatagc tagaagtcaa ggtgatttaa
    47821 gttatgtaaa atattttgct aatgagagct aagagcttct taacaagagt tttggtgtgt
    47881 ctcttgaaac aaggttaaca attaataaaa gacgaatggc agaagaaggc gtatgtaagt
    47941 ttaaaagaga taagttaaac aaactagata ctatgtaaga ggataagaaa cttatagaag
    48001 aatatgttac ttttgtaaag gatttagctt taaaatatag aatataaagt gattttaagt
    48061 aaaaattgta acaattaaat aagtataaga caagtttatg agttcatatt ttataacaag
    48121 gggtgatgtt atgaaaatac tacaacaatt taaaccatac aaatatgata aagaaaaaaa
    48181 tatagaatat gatgaaaaaa ttatagctaa tcaaaacaca gaagtttata tagtatatcc
    48241 acgtatatct gatgaagaaa ataggagaag atgggaagag ttcaatgaag tagctaaaga
    48301 agtagctatt aatatagcta tgaagaaagt tgagacagta gaaagttaag agaatagaga
    48361 agttgctaat acttagcact ttctcataga agaaaattgg acaactattt ttaaataatt
    48421 aggaataaaa aagttaaatc atatttgaaa aaaggcgata aaattgttga gtattgttat
    48481 tagtaataag aatatattaa ctatgtatta aaagaaatta taagttttta gtaaatatag
    48541 tatttaagga tattaaaact ggtaaaaaat atcatttaga ctattaaata aaaaagtgct
    48601 ggtcaaagta gcaacacaat aaataaaaaa acttatagaa attataagat aaaaagatga
    48661 gatttgaaat ggtcgaagtt aatataaaaa ttaaaggtga agtaagtgaa ataattaact
    48721 caattagagg tatatttaat agctttgatg aagaattaat attagatata aaatcagagt
    48781 aagaatataa aggtagatac gtagaaagtt ataagtaatt caagacttaa attaatttct
    48841 agttttttta atgcaaaaat ttaataaatt tcaaaatata ataattctga catctaaaaa
    48901 gagctattaa aaatgaataa tattgactat taagatatga atagtgtaca tatattaaat
    48961 taagatatca agtaggttaa ttttatataa tgatggggct caaattgaag aggcgatata
    49021 aaaatgtatt aatatatata aatataagaa ctataataca tattaactgt aaaattaagt
    49081 ttatagagtt atatttaaca tttaatgaac tgaaaaataa attagcaaat aatgaaaaaa
    49141 caacagtaat aaatgagaat aacaaagtat tttcaggatt agtggatttt tctaattgag
    49201 ctttaatcct agtttgttca atttcgatat ttctaatatt atcaatttat ttgtaataat
    49261 tatcaatttt tgcgaaatat ttttgataat ttatagaact ttatatattt tccttgaagt
    49321 atttttcgcc cttattgttt ggtttccaat tttaaaactc ataaactccc aataaattca
    49381 tgtataatat accttaattt ataatatatt taagagttta tttcagtaat ttttcgaacg
    49441 attttgatat atttcattta tagttttcaa aaacttatac aaaaaagagt gtttcatctt
    49501 tctaaacact ctttttataa gaatttgaat aataattttt taaaaattga taatatggtt
    49561 atttcttttt caagtttata gtttcctcat ttccattcca agatataatt agcttgtaac
    49621 tagattctct atttgaataa agacctggtc tttgtatttt tattgctttt tcttgacctg
    49681 cttctaaaat ttcatttttt acaactacaa ctgcatcttc accatctata aattgatagg
    49741 aataattaaa tttttgttca actacatctt tactattttt atattttatt tctaattttg
    49801 agtgtccttc agtagttaca aatttagctt tccaattttc tgattctcca ataaagacat
    49861 ttttatcatt ttttatatta agtatagcta tcacagagat tattaaaact ataagaatac
    49921 ctataaaaaa ttttttcctc atatcaatcc ctcctttgaa taattataac atgagaatag
    49981 ttattcaata ctaattttaa aaatattgtg gatttatatt ctattacaat agaatgtctg
    50041 ttataacaaa taaaaagtaa ggattaatat gaattatagg ttaaaaaaat ctgtaacaac
    50101 tattttatct ttactcataa tgacaatact attaccaaca aatgcaagtg ctagtaaggt
    50161 agaagattca tctatgaaag taatgtattc agagaaacaa gtaacagaca tggacaaatt
    50221 atacgatatg gctaaaaatg gaattactga tgttaaaagt tccaatgaaa aaggtataat
    50281 caaaaatgca agaacaggag agtccatata cgttgattct atatcaacaa ctcaattatt
    50341 agaagttaaa caatctaaaa atttaacatc aaagatatat tctaagacag tttttttgat
    50401 actaatacaa taaagcctct tagaaaagca agtatatcag aagatggatg ggataaaact
    50461 ggtggtgtaa aagcatctgg tacagttaca tatacaatat caaaaggaga tggagcattt
    50521 caatatattt ccgttagtaa agtttctgga aactggtcta ttgcagacgg ccagattcgt
    50581 gtaacaaata gaagggtgca tataaatcaa actggattta tttctaatga gctaggaatt
    50641 aagcagcaac aaaagaaaga gccaacatca aattctttct catataatgc accttctagt
    50701 tggaaaccag taggtagaga ttcagttcaa tacaattgtg ggtttgaatt aattgcaaca
    50761 ttagtaagag gaggaagttc ttgggcattc aatttcattg ttcaacctta aaatatttta
    50821 agtgaatttt attaaaatca aagatataca tatactttaa aattagagaa tgtaaattaa
    50881 tttgtgtttt taaagataat tcctatgtta tagtaacaat tgcttattta ctattaatat
    50941 ttcttaatat atgaatttta ttttaataaa ttataaaaaa tgtatattgg gatgttttta
    51001 aatatttttg gaaattatat atattataac aattatagca aatttttagg gtataatttt
    51061 taaaattata ttctactatc aaaagatatg aataaatgat tgtatatatt atatcaattt
    51121 ttatatttca tagtccattt tttcatcaca ttttcgcttg tcaagaataa tatctttaag
    51181 agtgaagcgt ttgatgtaaa tctggacttt gtttttgaga catttttgaa atatttatta
    51241 aatctttcga ttatacttgt aatatatatt atagatatta cttattttag taaaactaaa
    51301 ggatgatgct acaacattcc agttatttcc caaaatttta atttctgaag tatactgttt
    51361 acattatttt taatctattt tatctaaagt cgataaggct tcttattcat taactgacct
    51421 atatacagat ttaaaattac ttattaattc ttttgagtcc ttgtatgaaa catatttaaa
    51481 gtatttctta tctaatgtat taaacatttt tgtattacag aatgaggata acttgtagct
    51541 attgtctctt tgaaataagt catctgtgtt tactcctaaa actacttata tctgtagatt
    51601 ttataagttc tccatcttat ataatttctt tgattaattg ttttcttaaa ttattcataa
    51661 aaaaatctcc gttacaatat ttgattatat ttaaatcatt gctataaaga agacttttat
    51721 taactttgaa aaacaaatta ttttaattgc tggtgattag ttgctacctt cttttgatat
    51781 taattctatt agattttata tagttatata tctctttcta actaagtaat ataatattac
    51841 tgttatagta gctactaata ttattgctat tattgtaata tctaatcttt ttgtaaataa
    51901 tcctattgct atatattcaa aacctgttac atatcgataa agtacttcta gtttaatagt
    51961 tctttttcta tctatatttc catattttga ttctaattta ttaaaatatt tttcattaca
    52021 aaaaccaatt ttaatttcaa ttaaagatat tattgtagtt aatattataa ttcccaatcc
    52081 cataagtata agataaatgt tcctaaaatt catagaagat acctccttta taatataatc
    52141 atcaactcat gttgtattaa ttttcctaat caaagtataa cagtaacaca agtataaaga
    52201 gtatctaaaa tattacaaat attaaatgag aaaaagatgg taagttaacc ccaccatctt
    52261 attttttatc tactaatgaa tgatgctaca aaagttagta taaaaaatga aacaaacaat
    52321 ataatgaact tgtgtaattt acttagttgg aatgctttat tacaaaataa taaaattaat
    52381 gctgaaacaa ttgcaaaaag tagatatttt ataatcattt tatcacctat tgatttctac
    52441 agattgtaag tagtatttgg ttacacaagc acctccttta ttataacgta ttttttaaat
    52501 ttaaagattt ttccagatgt tatatttgac tcaaggtaca ttgtaattaa tctaactata
    52561 agtggcaagt actgtcctat accaggaata gcaccagcca aaagtcctga aatccctata
    52621 ccagcatcag ttaaagttaa tgcaactcct ttagaaagat ataaattaaa acctattcta
    52681 gcccaaacta ttttatttat ttcagctttt ctataatata attcaagtag tgtcaatatc
    52741 gttcttctta aggtttttaa ttagcttata tattgttatt gtata
//
LOCUS       DAEOSP010000003        50803 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.003, whole genome shotgun sequence.
ACCESSION   DAEOSP010000003 DAEOSP010000000
VERSION     DAEOSP010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50803
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.003"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..359)
                     /locus_tag="KRH54_000108"
     CDS             complement(<1..359)
                     /locus_tag="KRH54_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HBG8577793.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHI"
     gene            1181..1315
                     /locus_tag="KRH54_000109"
     CDS             1181..1315
                     /locus_tag="KRH54_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8577794.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGVSQTKGY
                     "
     gene            1667..2401
                     /locus_tag="KRH54_000110"
     CDS             1667..2401
                     /locus_tag="KRH54_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577795.1"
                     /translation="MTKQKKVIWIILGIIIFVFSVFLGLGYLGQITGGNSLIQRTEMN
                     DKYVPEEITKYYPIEDLNSKESLLSDKNYANSIQDALLSASIEFEQGEEYKTHIDKII
                     KEFENENYKSVLYISEKNDIESSLTFSKFKIKEVDGKKRYAHITSVHEVIKKDRPYDK
                     DTMSLLKSQLALSDRLQDLNISPDNSRFLYGFVHDEDIYNTKIENKKPDEIIYFELCE
                     KPFYFWYYENFQSDKSGKSLSIEIER"
     gene            2437..2745
                     /locus_tag="KRH54_000111"
     CDS             2437..2745
                     /locus_tag="KRH54_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577796.1"
                     /translation="MKKLYCMNCGTEIKGGIPNECSCGAEFDLADTKNIKRIITSIIF
                     FVILMSPLLILIHFGRQYLQASIILYALLLILGIFWCRIVQTILVRLGLVTMTNIEIK
                     "
     gene            3231..3698
                     /locus_tag="KRH54_000112"
     CDS             3231..3698
                     /locus_tag="KRH54_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577797.1"
                     /translation="MGRIIIVLYMEALLLSVLTIWFLYHIIKKKKIFRWENAPLDMRD
                     FVKRNEKRMNMISNGIIGIILIPAFLWVVPPAVQDFPNVSSDNYLEVEGTVTSWDYSD
                     EDKNKTRSIGIMDNKTKKEIFVTLYSKGIQKGEYLRVRYLPNSKYGEIKEKKR"
     gene            3695..4186
                     /locus_tag="KRH54_000113"
     CDS             3695..4186
                     /locus_tag="KRH54_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577798.1"
                     /translation="MKIIGLFFMTLVFGIISMITSKKYKLQGELKELCFPPVFSKILY
                     GCFIISMICSVVIIYLWQDTGITILFLVMGIISAFTAWAISKYKIIIEEQELIVIYPM
                     KKRKISITKIEYIEISTSGSMKIIVQNEKKINLDAMLIGTESFIEYMKKNGVLIQENK
                     GND"
     gene            4527..5693
                     /locus_tag="KRH54_000114"
     CDS             4527..5693
                     /locus_tag="KRH54_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG8577799.1"
                     /translation="MNDIKFIETLKQKRNACDYSQSRLALELQISRQNLNEIENGKTK
                     ASKEMKHILLHYLDYCNCTQPFTLTIDYLRVRFPTTDALEIIKNVLAMKSKYFIHEDY
                     GMFGYEEQYIYGDISVNASKDSSMGVLLELRGMGCRNLEYVLQARGIDWYSFLSCCID
                     YQGVFKRIDLAINDMGGLLDIEILRERYYANKVWKRSRTHEAVDSGKLSGTNGDTAKT
                     FYIGSKSSSIYFCLYEKEKEQKSKGIKTDIKNRFEIRLKNGKAEQTIEQLVFSRNPEQ
                     TIANLILTQIDFPDYILWDIFLDNVTTSLPFIMTPVAVNMDKTKRWLERQVMPSLLMI
                     KEIEKKTGAKYLEEIDRHTRLTEKQELKIKQMTTDIADMIEKDTAVPQRNDGIF"
     gene            6564..6773
                     /locus_tag="KRH54_000115"
     CDS             6564..6773
                     /locus_tag="KRH54_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HBG8577800.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            6885..8498
                     /locus_tag="KRH54_000116"
     CDS             6885..8498
                     /locus_tag="KRH54_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG8577801.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFERPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAYYGA
                     YAPLGYKKHPDIKGKLLIDDETKWIIEKIFSLAYQGYGSAKITKILREEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNRQSTISFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     ATNKANKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LHKIQKVGNAERIREKKKKASALRKAENRQNEIDRLFAKMYEDRACEKITERNFIMLS
                     GKYQKEQIELEQQITNLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEENERIQEIEIYYRFIGKVD"
     gene            8536..8934
                     /locus_tag="KRH54_000117"
     CDS             8536..8934
                     /locus_tag="KRH54_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG8577802.1"
                     /translation="MSVPDSDMLISISEVLETPVSTLLGETIVESEVDSLKVISEKLE
                     IINLQLAQRKNMRRKMFHWLFILLCAVILIGFVVLGGLNSPYLDWNYTDPETAVFGVA
                     FHAFEWLFVRLVPIVLIGAIIGIFVTRKKI"
     gene            9339..9875
                     /locus_tag="KRH54_000118"
     CDS             9339..9875
                     /locus_tag="KRH54_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG8577803.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVVIGILHN
                     REISMKEYIYFCYRQFKK"
     gene            complement(10309..11109)
                     /locus_tag="KRH54_000119"
     CDS             complement(10309..11109)
                     /locus_tag="KRH54_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8577804.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(11304..12902)
                     /locus_tag="KRH54_000120"
     CDS             complement(11304..12902)
                     /locus_tag="KRH54_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG8577805.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            13230..13406
                     /locus_tag="KRH54_000121"
     CDS             13230..13406
                     /locus_tag="KRH54_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577806.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            13580..14710
                     /locus_tag="KRH54_000122"
     CDS             13580..14710
                     /locus_tag="KRH54_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG8577807.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKSYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSDTAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            14951..15301
                     /locus_tag="KRH54_000123"
     CDS             14951..15301
                     /locus_tag="KRH54_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG8577808.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            15309..15758
                     /locus_tag="KRH54_000124"
     CDS             15309..15758
                     /locus_tag="KRH54_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBG8577809.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            15860..16435
                     /locus_tag="KRH54_000125"
     CDS             15860..16435
                     /locus_tag="KRH54_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG8577810.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            16428..17837
                     /locus_tag="KRH54_000126"
     CDS             16428..17837
                     /locus_tag="KRH54_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HBG8577811.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            18021..19454
                     /gene="eutA"
                     /locus_tag="KRH54_000127"
     CDS             18021..19454
                     /gene="eutA"
                     /locus_tag="KRH54_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBG8577812.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDETIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            19472..20836
                     /locus_tag="KRH54_000128"
     CDS             19472..20836
                     /locus_tag="KRH54_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBG8577813.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            20849..21730
                     /gene="eutC"
                     /locus_tag="KRH54_000129"
     CDS             20849..21730
                     /gene="eutC"
                     /locus_tag="KRH54_000129"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBG8577814.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNAYI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKGNNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            21750..22403
                     /gene="eutL"
                     /locus_tag="KRH54_000130"
     CDS             21750..22403
                     /gene="eutL"
                     /locus_tag="KRH54_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBG8577815.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            22414..23115
                     /locus_tag="KRH54_000131"
     CDS             22414..23115
                     /locus_tag="KRH54_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG8577816.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEITDNVSKNLSEKENVILDVNENSGNTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            23118..24587
                     /locus_tag="KRH54_000132"
     CDS             23118..24587
                     /locus_tag="KRH54_000132"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HBG8577817.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKSVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            24683..24970
                     /gene="eutM"
                     /locus_tag="KRH54_000133"
     CDS             24683..24970
                     /gene="eutM"
                     /locus_tag="KRH54_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBG8577818.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            25097..25858
                     /locus_tag="KRH54_000134"
     CDS             25097..25858
                     /locus_tag="KRH54_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--cob(I)alamin adenosyltransferase"
                     /protein_id="HBG8577819.1"
                     /translation="MGVLTESEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYRYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            25871..26500
                     /locus_tag="KRH54_000135"
     CDS             25871..26500
                     /locus_tag="KRH54_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG8577820.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            26542..27267
                     /locus_tag="KRH54_000136"
     CDS             26542..27267
                     /locus_tag="KRH54_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HBG8577821.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKEVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLISVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKV"
     gene            27280..27552
                     /locus_tag="KRH54_000137"
     CDS             27280..27552
                     /locus_tag="KRH54_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HBG8577822.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            27545..28096
                     /locus_tag="KRH54_000138"
     CDS             27545..28096
                     /locus_tag="KRH54_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBG8577823.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            28108..29199
                     /gene="eutH"
                     /locus_tag="KRH54_000139"
     CDS             28108..29199
                     /gene="eutH"
                     /locus_tag="KRH54_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBG8577824.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            29201..29674
                     /locus_tag="KRH54_000140"
     CDS             29201..29674
                     /locus_tag="KRH54_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HBG8577825.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(29794..29895)
                     /locus_tag="KRH54_000141"
                     /pseudo
     CDS             complement(29794..29895)
                     /locus_tag="KRH54_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            30190..30711
                     /locus_tag="KRH54_000142"
     CDS             30190..30711
                     /locus_tag="KRH54_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8577826.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            31090..32265
                     /locus_tag="KRH54_000143"
     CDS             31090..32265
                     /locus_tag="KRH54_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8577827.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            32300..33838
                     /locus_tag="KRH54_000144"
     CDS             32300..33838
                     /locus_tag="KRH54_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG8577828.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            33870..35072
                     /locus_tag="KRH54_000145"
     CDS             33870..35072
                     /locus_tag="KRH54_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG8577829.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQKKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(35239..35838)
                     /locus_tag="KRH54_000146"
     CDS             complement(35239..35838)
                     /locus_tag="KRH54_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8577830.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            36008..37366
                     /locus_tag="KRH54_000147"
     CDS             36008..37366
                     /locus_tag="KRH54_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577831.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            37359..37991
                     /locus_tag="KRH54_000148"
     CDS             37359..37991
                     /locus_tag="KRH54_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG8577832.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            38320..39477
                     /locus_tag="KRH54_000149"
     CDS             38320..39477
                     /locus_tag="KRH54_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8577833.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(39624..40259)
                     /gene="lexA"
                     /locus_tag="KRH54_000150"
     CDS             complement(39624..40259)
                     /gene="lexA"
                     /locus_tag="KRH54_000150"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBG8577834.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(40363..41490)
                     /locus_tag="KRH54_000151"
     CDS             complement(40363..41490)
                     /locus_tag="KRH54_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG8577835.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(41774..42619)
                     /locus_tag="KRH54_000152"
     CDS             complement(41774..42619)
                     /locus_tag="KRH54_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577836.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(42649..43020)
                     /locus_tag="KRH54_000153"
     CDS             complement(42649..43020)
                     /locus_tag="KRH54_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG8577837.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            complement(43173..43433)
                     /locus_tag="KRH54_000154"
     CDS             complement(43173..43433)
                     /locus_tag="KRH54_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBG8577838.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(43622..44566)
                     /locus_tag="KRH54_000155"
     CDS             complement(43622..44566)
                     /locus_tag="KRH54_000155"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBG8577839.1"
                     /translation="MIENNSEKIKILENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(44587..45441)
                     /locus_tag="KRH54_000156"
     CDS             complement(44587..45441)
                     /locus_tag="KRH54_000156"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBG8577840.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            complement(45456..46817)
                     /gene="accC"
                     /locus_tag="KRH54_000157"
     CDS             complement(45456..46817)
                     /gene="accC"
                     /locus_tag="KRH54_000157"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBG8577841.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(46833..47285)
                     /gene="accB"
                     /locus_tag="KRH54_000158"
     CDS             complement(46833..47285)
                     /gene="accB"
                     /locus_tag="KRH54_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBG8577842.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(47555..48001)
                     /locus_tag="KRH54_000159"
     CDS             complement(47555..48001)
                     /locus_tag="KRH54_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577843.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            48276..48554
                     /locus_tag="KRH54_000160"
     CDS             48276..48554
                     /locus_tag="KRH54_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577844.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(48683..49429)
                     /locus_tag="KRH54_000161"
     CDS             complement(48683..49429)
                     /locus_tag="KRH54_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577845.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(49558..50202)
                     /gene="trmB"
                     /locus_tag="KRH54_000162"
     CDS             complement(49558..50202)
                     /gene="trmB"
                     /locus_tag="KRH54_000162"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBG8577846.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(50340..50660)
                     /locus_tag="KRH54_000163"
     CDS             complement(50340..50660)
                     /locus_tag="KRH54_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBG8577847.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
BASE COUNT        19192 a         6764 c         8203 g        16644 t
ORIGIN      
        1 tatatatgtt taattccagc ttgaattata gccttagtac aatttaagca aggaaagtga
       61 gttacataaa tagagcaatt tttaactgaa ataccttctt ttgcacaata ataaagggca
      121 ttttgttcag cgtgaatggt tctaatacaa tgtccatctc tcatttcatg accaacttca
      181 ctacaatgct tatctccaga aatactacca ttatatccag tagacactat tctgttttct
      241 gaattgacaa ttatagctcc tacataagct ctatcacaag ttcctctttc agctactgtt
      301 tcgcacagtc tcataaaata ttcttgccat gagcatctat tttcaagttt tttattcaaa
      361 ttatatacct ccaaaactta tatctaatat ataagattta taccttaaaa ataatattaa
      421 aaacaaattt atacgataaa taaaatttat taatactgtt tttagtctaa attatttcat
      481 tttaaattaa agttacaagg aatctaaaat tatatatatc tttcttcgta atattaatgt
      541 tacaatataa aaaactgtct caataaaata gagacagttt tacagtcaaa gtttataata
      601 caagttcata tttttcttta aattctttta cagcttcggg ccatgccttt tgttctgtaa
      661 tacctttttc tttagctatt ttaattgcaa tctctcttat cattgcagcg ccttctaata
      721 ttcccatttt taatctccct tcaaatatct ttttgtatag aatataaata gtgctaagat
      781 ttattacatg ctgcatatac aatatctata caacacataa cttgtaaatt ccttttattt
      841 tcatacgtct ttaaccgaca tataattata ttcaataagt tttaaaatta tgaactcaat
      901 tataattatt tttatttttt tagaatctaa acactaaaaa ttgtaggtaa atttgtctta
      961 tgcctttata aatatttcta cagttttaat tactggacat atctattttt attataaaaa
     1021 gcgcttaaaa atcgagaata tccgcataaa aactgtatat aagcaaaacg gttaaaggaa
     1081 aaagaagaaa tttacttgaa tgttaaaatg cgttgcttga agcaatgggc agttttgcct
     1141 ataatatcgg taacaactga aagaaaggtg gttagcccta atgttaagcg aaaacattaa
     1201 aacaatcagg aagtcaaaag gactttcaca acaagaactt gctataaaac tgaatgtagt
     1261 gcgacaaaca atatctaaat gggaacaagg agtttcccaa acaaaaggat attaagaaat
     1321 aacctgtgtt ttcctgcttg cgaataaaag aatagtgtga ttaagttaga aatactgcat
     1381 aaatgtgctg gtatctcttt ttcgttgcct tgaaacagca gactatttca tttctgcttg
     1441 attactctaa acttatcagg ctgtggtttg tcaagggctt gttgcgtaag caatgctttg
     1501 gcacccttta caaattatgg acggtaaagt agcttttcaa aagcagaaaa aagtagtctt
     1561 aagattttga aatattgtag cgggtagtgg aaaatggtat aatgtgaata cagagaaaca
     1621 tataaatttg tatttaacaa tcaataaatt gagagggctt aatcttatga ctaaacaaaa
     1681 gaaggtaatt tggataatat tgggaataat tatttttgtg tttagtgttt ttctagggct
     1741 tggatattta ggtcaaataa caggtggaaa tagtttaata caaagaacag aaatgaacga
     1801 taaatatgtt ccggaagaaa taacaaaata ttatccaata gaagatttaa actcaaaaga
     1861 aagtttactt tctgataaaa attatgcaaa ctcaatacag gatgctctgt taagtgcctc
     1921 tattgagttt gaacaaggcg aagaatataa aacacatatt gataagatta taaaggagtt
     1981 tgaaaacgaa aactataaaa gtgttttgta tatatcagaa aaaaacgata tagagtcaag
     2041 tttgacattt tcaaaattca aaattaagga agtagatggt aaaaagagat atgctcatat
     2101 tacaagtgta catgaggtta taaagaaaga ccgcccgtat gacaaagata cgatgtcgtt
     2161 actaaaatca cagttagcat taagtgatag attgcaagac ttaaatatca gtccagacaa
     2221 tagtcgcttt ttatatggat ttgtacatga tgaagatatt tacaatacaa aaatagaaaa
     2281 taaaaaaccc gatgaaatta tttattttga actttgtgaa aagcctttct atttttggta
     2341 ttatgaaaat tttcaaagtg ataaatctgg aaaatcttta agtatagaaa ttgaaagata
     2401 ataatagaac aattccaggt tgtagaggtg attagaatga agaaactata ttgcatgaac
     2461 tgtggtactg aaataaaagg aggaatacct aacgaatgtt cttgtggagc tgaatttgat
     2521 ttagcagata caaaaaacat aaaaagaata attacaagca ttatattctt tgtaatatta
     2581 atgtctccac tgcttattct tattcacttt ggcagacaat atttacaagc gtcaattatt
     2641 ttatatgctt tgctcctgat tctaggtatt ttttggtgtc gtatcgtaca aactatttta
     2701 gttcgtcttg ggttagtaac tatgacgaac attgaaatta aataacaaca ataacatctt
     2761 cacaactcta ctgagcagga aatctgaaaa ggtctcctgc ttttttatac caaaaaagat
     2821 taaaaatcct attatccgca taaacgcagg aactttaagc aatatgaatg aaaatacaaa
     2881 agaaatttac cactaattta ctacgattga aagaaccttg atacgacaaa gaaaatagca
     2941 taaagaaaag caaaatggca tttcatacaa aatatgagat gtttttttgt tactgtaaca
     3001 cagcataact tgttcttttc tgcttgatta tctaccattt tttagagcat ggattgtcaa
     3061 gagcttgttg cgtaagcaat ggttgcactc ttgataaggc atgggataaa aaatattctg
     3121 gcagatagca gaaaagtaat gacatgggaa ttctatgaat gatataataa gttatcagat
     3181 aaaaaatcaa tcgtttaata atttctatta gacttctagg agaaaagagt atggggagaa
     3241 taattatagt tttatatatg gaagctttat tgttatctgt tcttacaata tggtttttat
     3301 atcacataat aaaaaagaaa aaaatctttc gttgggaaaa tgctccattg gatatgagag
     3361 attttgtaaa gagaaatgaa aaaaggatga atatgatttc aaatggtatt ataggtatca
     3421 ttttgattcc tgcattttta tgggtagtac ctcctgctgt acaggatttt ccaaacgtaa
     3481 gttctgacaa ttatcttgaa gtagagggaa cagtaacatc ttgggattat agtgatgaag
     3541 ataaaaacaa aacaagatct attggaatta tggataataa aacgaaaaaa gaaatatttg
     3601 taactcttta tagtaaagga atacaaaagg gggaatatct gagagttaga tatttaccta
     3661 atagtaaata tggagagatt aaggagaaga agagatgaaa ataataggat tattttttat
     3721 gacattagta tttggaataa tatctatgat aacaagcaaa aagtataaat tgcaagggga
     3781 attaaaagaa ttatgctttc cacctgtatt ttctaaaatt ttatatgggt gtttcatcat
     3841 ttctatgata tgttcagtgg ttattatata tttatggcaa gatactggta ttactatttt
     3901 gtttttggtt atggggatta ttagtgcttt tactgcatgg gcaatcagta aatataaaat
     3961 aattatagaa gaacaggaat tgattgttat ttaccctatg aagaaaagaa agatttcaat
     4021 tacaaaaata gaatatattg aaatcagtac aagcggtagt atgaaaatca tagtacagaa
     4081 cgagaaaaaa ataaatttag atgcaatgtt aatagggact gagtctttta tagaatacat
     4141 gaagaaaaat ggagtactta tacaagaaaa taaaggaaat gattaaacag acggagtgat
     4201 ccagacacgg tggcaggaaa atctcccaga tttttctgcc atgtcttgca agtggctggt
     4261 tcacttcgtc tgtgagcgag cctgtggcga gcgaagatta agcccccgtt atctaacagg
     4321 ggggtactat tacaaaagac aagagcaaaa acaaatacaa aactgctgat atgcaacgat
     4381 ttataaagtt ataagaactt atttcacttc acaccctatt ctttttaccc gacaggtcta
     4441 aaacagcata tcaatagaat ataaatataa gatacaaggt gtatgctcct atgagtatat
     4501 gccttttttg attggaggaa taaagcatga atgatataaa atttattgaa acattaaaac
     4561 aaaagagaaa tgcctgtgat tattcgcaat cacgactggc actggaacta caaatcagca
     4621 ggcagaactt aaatgaaatt gaaaacggaa aaacaaaagc cagtaaagaa atgaaacata
     4681 tacttctaca ttatcttgat tattgtaatt gtacacagcc tttcacttta acgattgact
     4741 atctgcgtgt tcgttttcct accacagacg cattggagat tataaaaaat gtactggcaa
     4801 tgaaaagcaa atattttata catgaagatt atggaatgtt tggttatgaa gaacagtata
     4861 tctatgggga tattagtgta aatgcttcta aagatagttc tatgggtgtt ttattagaat
     4921 taagaggtat ggggtgtcgg aatttggaat atgttttaca ggcaagaggg atagactggt
     4981 attccttttt aagctgttgt atagattatc aaggcgtttt taaacgtata gatttagcaa
     5041 tcaatgatat gggcggattg ctggatatag aaattctaag ggaacgctat tatgccaaca
     5101 aggtatggaa acgttcaaga acccatgaag cagtagacag cggaaaatta tcgggtacaa
     5161 atggagatac tgcaaaaact ttttatattg gttcaaagag tagttctatt tacttttgct
     5221 tgtatgaaaa agaaaaggaa caaaaaagca agggcataaa aacagacatt aagaaccgct
     5281 ttgaaattcg tttgaaaaat ggaaaagcag aacaaacgat agaacaactt gttttttcaa
     5341 gaaatcccga acagaccata gcaaatctta tcctcacaca aatagatttt cccgattata
     5401 ttttatggga tatattttta gataatgtaa cgacttcact tccatttatt atgacacctg
     5461 ttgctgtcaa tatggataaa acaaaacgct ggttagaaag acaggtcatg ccctctttat
     5521 tgatgataaa agagattgaa aagaaaacag gagcaaagta tctggaagaa atcgacaggc
     5581 atacaagact aacagaaaag caggaattaa aaatcaaaca aatgacaaca gatatagcag
     5641 atatgattga gaaagatacc gctgttccac aaaggaatga cggtattttt taatttcttc
     5701 aaaaaatctg caacaaaacg gctacttgtg tacagatatg gtgcgaggaa aggaaatcta
     5761 actttttaaa taaggtcata aaatcttcct ttggtgtaca gatacggaat gtaagaagaa
     5821 attgaaattt ttttgaaaaa cctgcaacaa aataagactt ctattcctta tatggtgcga
     5881 ggaaagaaaa tcgaaacttt ttttgaaaac aggtcatcaa atcggtggtt gctgtcctta
     5941 tatggtgtga aagaagaaac tattttcatt tttaacttaa caatccgtag tttccgttcc
     6001 ctatatggag caaagaaaat ctttcattcc atttttggca ggaacggtag tgtatgggga
     6061 gttagggcgt ggaaagaaaa acaatctttt ttcatcatca acttagcaaa ccgatacttg
     6121 ctatccctat atggtgcatg aaaagaagtt aaaatttttc tatttcaact tagcaaatgg
     6181 acgtttgctg tacagatagt agtgaaaaag ggaaaaagtt ttgaatttta gttacatttc
     6241 tcctcttttc caacgcggta tataggaaag atgattttct tctttcaatc gttctttgac
     6301 aactgaataa agcaattcaa tacgtttgac agacagcgag ccgttgaaac agatacgcca
     6361 tgacctttcc cctgcaaaag cgagcgataa cctatctgtg ttcttgtaaa agacttgtcc
     6421 tcttttatcg ctatgacctg tgctgtcaga taatgatact cccgtacagc cacagcttga
     6481 gcgttcagag cgtcgcacgc aatgggtacg gctacataag aactatgcag aggtggaact
     6541 cctgtgggct atgacaaaag ccgttgaatt gcttagaaag attttacaga aagggggtat
     6601 gtgttattga taatgctgta aaaacaattc aaaagaaaaa tggcaagtgt ggcattggca
     6661 acagaggaaa gacaaagatt gttgtaaaag agcatttttc cgaaaaggga aagacaatgg
     6721 aagaacttct aacggatgtc atgttggaaa aagcaaagca gacaatcgca taaaatcggt
     6781 gcagttgtaa gaaatagatt gaatgacaaa aagtacccgt gttatacttt acttgcaagc
     6841 aggtattgta aacacgggtt agttataaag gaggataact gacaatgaaa caacagattt
     6901 acaatactgc actttatctt aggttaagcc gagatgatga attacagggc gaaagttcca
     6961 gcattaccac acaaagaagt atgttgcgtc tatatgcaaa agaacatcat ttgaatgtga
     7021 ttgatgaata tattgatgac ggctggtcgg gaacaaattt tgaaagaccg agctttcaaa
     7081 gaatgattga ggatatagag gcaggaaaaa tcaactgtgt tgtaacaaaa gacttatcac
     7141 gacttggcag aaattatatt atgacagggc aatatactga attgtatttt cccagccata
     7201 atgtccgtta catagcgatt gacgacggtg tagacagcga aaaaggcgaa agtgagattg
     7261 caccatttaa gaacatcatc aatgaatggg tagcaagaga tacgagccgt aaagtgaaat
     7321 cagcgtttaa gacaaaattt gcagagggtg cgtattatgg ggcttatgct ccgttaggat
     7381 ataagaaaca ccccgacatc aaagggaaac tgttgattga tgatgaaaca aaatggatta
     7441 ttgaaaaaat cttttcccta gcttatcaag gttacggaag tgcaaaaatc acaaaaatac
     7501 tgcgagaaga aaaagtcccg acagcgtctt ggctgaattt tacaaggtat ggtacttttg
     7561 ctcatatctt tgagggaaag cccgaaagca agcgttatga gtggacgatt gctcatgtaa
     7621 aggcgatatt gaaaagtgaa gtctatatcg ggaacagcgt tcataatcga cagtcaacca
     7681 tttctttcaa aagcaagaag aaagtacgaa aacccgaaag cgaatggttt cgagtagaaa
     7741 acacgcatga accgattatt gacaaggaag tgttctatcg tgtacaggag cagataaaat
     7801 caaggcgtag acagacaaag gaaaaggcaa cgccgatatt tgcagggctt gtcaagtgtg
     7861 cggattgtgg ttggtctatg aggtttgcga caaataaggc aaataaaaca ccgtatagtt
     7921 attatgcttg cagttactac gggcagtttg gtaaaggtaa ttgttctatg cactacattc
     7981 gttatgacgt gctgtatcaa gctgtattgg aacgattgca gtattgggct aaggcagtac
     8041 agcaggacga agaaaaggta ttgcataaga tacagaaagt cggcaatgca gagcgaatac
     8101 gggaaaagaa gaaaaaggca agtgccttga ggaaagccga gaaccgacaa aatgagattg
     8161 accgtttatt tgcgaaaatg tatgaagata gagcctgtga gaagataacg gaacgaaact
     8221 tcatcatgct gtcggggaaa tatcaaaaag agcagataga actggaacag cagataacca
     8281 acctaagaga agaattaagt aaaatggaac aggatatgat aggtgctgaa aagtggattg
     8341 agttaatcaa ggagtattcc gtaccaaagg aactgacagc accattatta aatgcaatga
     8401 tagaaaaaat ccttattcat gaggcaacaa cgaatgagga gaacgaaaga atacaggaaa
     8461 tagagatata ctaccgattt attggaaaag tagattgata aagagggttc atatctttaa
     8521 ctaagggaaa gcggactgtc agttcccgat tctgatatgt tgatttccat atcagaggtg
     8581 cttgaaacgc ccgtaagcac tttacttgga gaaactattg ttgagtcgga agttgatagc
     8641 ttaaaagtga tttctgaaaa attagagatt ataaacttgc aacttgcaca aagaaaaaac
     8701 atgagaagaa aaatgtttca ttggttattt attttattat gtgcagtcat attaataggt
     8761 tttgtggtct tagggggact aaatagtcct tacttggatt ggaattatac cgaccctgaa
     8821 accgctgttt ttggagtagc ttttcatgct tttgagtggc tatttgtcag attagtaccg
     8881 attgtcctta taggagcaat tattggaatc tttgtgacac gtaagaaaat atgaagtaac
     8941 tctgcttttt ataatacagc aagcgttcct taaaagcgtc attctacatg gcaatcgagg
     9001 acgactatgt aaggaagaac ctttttgatt tccaactaag cgaagttatt gaggacgaca
     9061 gtaagaccaa aaccgcatta agtaaagtac aggaagcaag actgctggac ttatactctc
     9121 atgcgtcaga tgcaggaaat gcgaggtctg gtagcatgat ttacaacgat ttgtacaacg
     9181 actgtgagta gaaatatatg gagataagta ttatctacga aatcagaaaa gaaatgactt
     9241 gataatttac tcatgttata attattgatg tgaaattaat aaatcgaaat tccttttttg
     9301 gatttaacaa tactgagtaa ggaaaaagtg aatgactttt gcaaggaggt gttaatatca
     9361 ggcaagtaaa gtcttttgtt tttccagtta tatccttaat attcttagac caaattagca
     9421 aagttcttat aggattattc ttaatggact ttgaaattga tataattggg aaatttttaa
     9481 gattcaatcc tgttcaaaat acaaatctct cttatggggg aaactttatt ggtattctat
     9541 ctaatttatg ggtattggta ttgtttaaca ttttagttat attagttatt atatctggat
     9601 atgcttttta taaatcaaaa aatgaacaaa caagctattc agtaaaagta attatgtctt
     9661 gtggacttgc tggtacaata tgtagcttga tagataaatt attttgggga ggaagtttag
     9721 attttttgca gataccaagc ttttttattt tcgacttaaa agactgctac cttactgttg
     9781 cagaaataat atttgttgtc ataggaattt tgcataatag agaaatatca atgaaagaat
     9841 acatatattt ttgctatcgt cagtttaaaa aataaattta gaagataaat ttaagtttca
     9901 aggtaaactt atttaatcac agcaaatagt tggcttactt aagcttttgg gctaaactgt
     9961 gagaactaat taagtctctg ctatctaaca aacagagcac aaaaataaaa attaaatatt
    10021 aaatataaag cacagttaat tttgattaaa aagttgactg tcttttcttt tgtagaaatt
    10081 taagcatagg taataaagaa atgactgcat ttaagcatta tgtatataat tggaggcaat
    10141 aaaaagccag caaagcccat aaaatagagc tgtgctagca acatgatgag ttataagaag
    10201 ataatcaaaa acttacgctt tattttaatt taaaaaaatg attactgtaa agaaatcatt
    10261 ttttattata atcactgata catcaaatat tctaaataaa atttttattt atctggaatt
    10321 gtatattttt tctataatat taactacagc gtacattgcc caagcacaga tagccaatac
    10381 aaatactccc atcattacta aatctaattt aaatacttga gaaccatata ctatcaagta
    10441 gcctattcca tatcttgaaa ctaaaaattc tccaactatt acacctaccc atgccatacc
    10501 aatattaatt ttagttaaat ttattaaatt tcctgtgttt gcaggtaata tgagtttaaa
    10561 taatatttga gatttagtag ctccaaagct ttttaacatc ttaatttttt cttcatctat
    10621 attcataaaa taattataag ctgaaagtat agtaacaaca acagaaatcg ttacagctgt
    10681 tacgacgatt ccttctattc cagcacctac ccaaactata attataggag ctaaagcagt
    10741 cttaggaaga gcatttaaaa ctaccaaaaa tgggtctaat atttttgata atttttctga
    10801 ccaccaaaga gcaattgcta ccaaaatacc taatacagta cctataacaa gaccaagaac
    10861 agtttcatat acagatattc caatatgttt aaataattga ccactaaatg catattgaat
    10921 aaataaattg taaatatcac ttggtttgct aaatagaaat gtatttataa catttaagtc
    10981 agctaaaagt tcccagatta caataaaacc aattaaaatt aatagctggt agaataatac
    11041 atttcttttt tctcttttta cacctttaac atagtttata tatccttcag atcttttatt
    11101 aagcctcatg tgtatctagc tccttccata atatgtcgaa atacttttga aaatcaggaa
    11161 ctttacgtgt tttgagaggc gtttttgttc catccatgtt aagttcaata tcgtaaatat
    11221 tttttatggt agctggtctt ttagatagta ttgcaacctt atctgatatg cttattgctt
    11281 ctgctatagc taaaaaagtg caactaattg cttaaagaaa cagaattatc cacttcagca
    11341 cccatataaa caatattaag atttcctaaa ctgctatccc aaacgatact attaataata
    11401 gtagataata acaatctctt atcatctaaa ctggcattat caacttcatc attaaatctc
    11461 ttcaaattac taacaataat atcaatatta atactgtcac tactaatatt gatgttacta
    11521 gattccaaaa tattaatctt atcctttaaa atactaatct cattatttag gctctcaatc
    11581 tgggaaacta tataatcaga agcagtacta ccagtatttt ttgcaagctc tataactaaa
    11641 tttttaataa gtctttcctt atctaaaatt tgatttttaa gtaaattagt ctcagattta
    11701 aaaatattaa aaacattatc agtcttaatg ttattactat tgaatgcact tataatagac
    11761 tcttctttac aattcttaat tttatctata actaaacttt caactaatgg accattaaga
    11821 tttttacagt tgcaactatc agacccaagt gtttttttag taccacaaat ataataatac
    11881 tttaaatcct tgtcagtttt tttgccttta taggtaattc tcatattaga accacattta
    11941 ccacatttta taagaccaga taaaagagca gtttttccag ttgatatcct tggagtttta
    12001 tcacgatttt tatctaattg attttgtact tcaagccata tttcaggttc tatgactcca
    12061 tggtgttttg caattgctgc tattgagtta gatgtatttt tagcataagt aaggattcca
    12121 cattttctat ctggagtacc tacaacatcc ataccactat ttttaaggta atctaaaatt
    12181 tcatcattag cttttacata tgctggattt ctaagtattc tagataaaga actagggtca
    12241 aatttaccac cacgaggacc ccttattcca ttattataga catatttata taacttagat
    12301 aaggacctaa gttctaaata tttattgtat attagcttta caatctccat ttgttcatca
    12361 tttacttgaa gcttatacat acttctttct tttagatttt catctaagaa tgtgattttt
    12421 tcactgttga atccatgagg tgtattgcct ccaagccatc taccactacg ggctaattca
    12481 tacatattgt cttttatacg ttctgctatt gtttctcttt ctaattgagc aaatacactt
    12541 gatataaaca tcatagctct tcccattgga gttgaagtat caaattgttc tttgatagat
    12601 ataaatgata tgtttagttc atttaatttt tctattaaag tagagaagtc agatacattt
    12661 ctcgatatcc tatctaatct ataacaaact aggtaactaa atttcttatc tttggcatcc
    12721 tgcatcattt tcttaaattg tggtctatct atattgctac cactaaatcc ctcatcttca
    12781 tatatcaaaa aatcttttac accaatagac tttgcataat caatacataa ttgtatttga
    12841 ttttctatag aatcaccttt tccagtaaat cttgattttc ttgaataaat tgcagctttc
    12901 aaaattaaaa ccccctataa gttaggatat atatttgaaa ttatttatat acaacctttt
    12961 aagtaaactt attagaatga atttattaat taggtaaatt tatataattc tactttttat
    13021 aatatatgac tatttatttt atttctttac tattcaagtt ctaaatcaat aataaatttt
    13081 gatatttaat tgtatcagga caaatctcta atttcataga ttattaatat aaaatcatag
    13141 gttagtgtga aaaagttgaa atttaaatat tttaaaagat aatttttttg aactaatagg
    13201 tataacaaaa atttataggg ggattgcata tggaaatcaa tgttatagaa attttcccta
    13261 aagataaagc taaacttaat aaaatagaaa tggataaagc tagttggttt gtaaatataa
    13321 taagtaaaaa atatcctaaa gaagctttaa atgaagcatt tagtacttta gaaaaagaat
    13381 taaatataag taaagctaat acataagctt tgattattga ttttatatac tattggtttt
    13441 atacgctatg acttgacatt atattaatca aatagtatat ttttaaatat agcacaatga
    13501 agtgttaaaa gagcaatgga gctccataga taaagtctat ggagcttttt tgcgtattta
    13561 acttaaggag gataggactt tgaataactt tgagattaaa actagaataa aatttggaga
    13621 aggctcacta aaagctttgc aagaattaaa aaacaaaaat gtactaataa taacagaccc
    13681 ttttatggtg aaatcaaaaa caatagataa aataacatct aatttaataa actcaacata
    13741 tcaagttttt gaagaaatag taccagaccc accaatagaa gtagtagtag caggaataga
    13801 agcatttaaa tctataaatc cagacgtaat aatagcatta ggtggcggtt ctgcaataga
    13861 tgcagcaaaa tctataatgg acttttctaa aaaaatatta aaaattaacg aagtagagtt
    13921 tatagcaata ccaacaacaa gtggaacagg ttcagaagta acatcatttg ctgtaataac
    13981 agatagtcaa aaaaaatgta agtatccatt agtgagtgat gatttacttc cagatatggc
    14041 aatactagac ccagagcttg taaaaactgt accaaatttt ataacagcag atacagggat
    14101 ggatgtattg acacatgcta tagaagctta cgtatctaca aattctaatg acttttcaga
    14161 tgcactagct gaaaaagcta taaagttagt atttaaatac ttaataaaat catataaaga
    14221 tggaaatgac atagaagcta gagaaaaaat gcacaatgca tcttgtttag caggtatggc
    14281 atttaaccaa gcatcacttg gcataaatca ttctattgca catgtattag gtggaaaatt
    14341 ccatatacca catggaagaa caaactctat attacttcct catgtaatag aatataattc
    14401 atcaatagta ggatatacta acacaactta ctctgataca gctaaaaaat atgcacaaat
    14461 agcagaatta gtaggacttg ggagtgctaa tataagaata tctgttagaa atttagtaaa
    14521 tgaaataaag aaactacaaa aagaaatgaa tatgccaact aaattgacac aatgcaaatt
    14581 aagtttagaa gatataacta atgaagaaag agaaatagca aaacttgcta taaaagatac
    14641 ttgtacatta actaatccta aggtaccaac tgaagatgat ataataaaca tattaaagaa
    14701 cattaaataa ctacatttta ttacatgtaa atataatatt atacaaatag ggaaaatgtt
    14761 tttaaaaata taaattatat attgacaaat ataaaacata aaactataat gaaagtatca
    14821 caaaggcgtg atactttagt agaaagcaat ggagcttctt aggaatatta atattattct
    14881 tatggagctc cttttatttt aatcaaatag ctttttaata aactatattc aaagaaagca
    14941 gggaataaat ttgactgaag aatctaagca aagagttatt caagaatatg ttcctggaaa
    15001 acaggttaca ttagcacata tcatagctaa ccctaatgaa gacatatata agaagttagg
    15061 attagtactt gataaaaaag atgcaatagg aatacttaca ataacaccaa gtgaagcgtc
    15121 aataatagca gcagatgttg ctacaaaggc ttcaaatgta tctttaggtt ttatagatag
    15181 atttagtggt tcagtagtaa tatcaggtga tgtaagttct gtagaatcag cgttaaatga
    15241 tgttttagaa gtcttaggaa atatgttaaa cttctcatct acaaaaatca caaggacata
    15301 ataatacaat gaagaatata atatttatgg gaaagacagg tagtggtaaa actaccttat
    15361 gtcaaaaact agaccagtta gaattaaagt ataaaaaaac tcaatcagtg gaactttata
    15421 ataatgcaat agacactcct ggtgagtata tggaaaatag aaacctatat aatgccttaa
    15481 taacaacatc agcagatgct aaaattatag caatagtgta tgatgccaca caagaagaaa
    15541 attatatagc accaggtttt gccagtatgt tttgcaaaga cgtaatagga attataacta
    15601 aaataaataa agctactgaa aacgaagtca atatagctta tgaaagacta tcactagcag
    15661 gagcaagtca aatctttaaa atagacacag ttgataatat tggacttgaa aaattattta
    15721 aatatctaga aaaatataaa aatatagatg aagtttaagg ggaaatattt ttttattcaa
    15781 aaatattatt aaagtatgat aaaataaaaa aatgtataaa ctattttaag aagatttaat
    15841 ctttaagcga ggtaggaaca tggggaaaaa aatactattg gttgatgatg aaccactgtt
    15901 aaggatggat acaaaagata tattagaaag tcatggctat gaaataatag gcgaagcaag
    15961 tgatggattt gaagctgttg aaatgtgtaa aaaacacaaa cctgatttag ttataatgga
    16021 tatagagatg ccaatacttg atggtataaa ggcaggcaaa ataatatgta aagaaaatct
    16081 agctggtgga gtacttttat taacttcatt tgaagataaa gaatacgtag aaaaggctaa
    16141 aagtataggt gcttatggat atttagtaaa aacagcaagt gaaaaatcac ttatcccaac
    16201 catagaaatg tgtttaaata aggtaaatga atttgaaaaa atgaaaaaag atttagaaaa
    16261 agtaaattct aaattgaatg aaagaaaatt agtagaaaga gcaaaaggta tacttataag
    16321 agagtttaag atttctgagg atgaagcata cactagaata agaaaattaa gtatggataa
    16381 aagaactaca atgatggaaa ttgccaaaat gattataata ggatacgatg actaatacga
    16441 taaaaaaaat atcaactgca caaacatact taaataataa cgaaattgaa aaaattatag
    16501 aagtatcaaa atccttagat ttaatggctt ctttttatga agctgatgtt tttatagatg
    16561 tactaagtaa atttgatgat gaagcaattg ttgtggcaca tggtagacct aaagaaaagt
    16621 caatgtactg tgaaaatgtt ataggtaaaa aggctttaat tgaaaatgaa ccaggagtta
    16681 taaagtgctt aaaaacagga gaaagagtaa gagatattaa agctttaaca caggaatata
    16741 aactggtcaa acaaaatatt cagccaataa cactagatga caaagtttta gcagtactaa
    16801 tagttgaaaa agatataagt agtgaagtta agaatgagtt taatgctagt gaagacgaag
    16861 aaaataaaaa tttacatcag tttataaaac ttattggaaa taatgacttt ttagttgata
    16921 atttaaattc agccatatta atatttgata aaaatggaaa attaaaaatt aaaaatcgta
    16981 ttgcaactga gatatacaaa aatcttgggt ttggatatga tatacaagat atgcattaca
    17041 atgatttaga tatagaaacc aacacctttg aaaatatatt aatggatgaa aattatgatg
    17101 agacaaatga agtttcaata ggtaatatgt tttttaaaat aaagaccata tacatcaaag
    17161 atgatgaatt taaggtttgc aaaatagtac aagatataag tgatttaaag agaaaagaag
    17221 aagaattagt tttgaaaaca gtggctataa gagaagctca tcatagagta aaaaataatc
    17281 ttcatacagt aatatctata ataaaaaaac aaagtagatt atctcaaaat gatgaagtaa
    17341 agcagtgttt agataacata ataaataggg tatttgcaat actttcgact cattatttac
    17401 tatctaaaga agttgataat aatatatcta tacgagaagg tatcaattta ttagtgtcaa
    17461 atattcaagg tggctattta tttagtaagg atatagatat acgtataact ggtgaagatt
    17521 ttaaaattag tggagaaaaa gcaacagcaa tacttttggt tataaatgaa atactacaaa
    17581 attgttatga ccatgcattt tcggatagag aacatggtac tatacaaata ttaataaata
    17641 aagataacga ctatagaaat attgctatta tagatgatgg aattggattt gatagtaata
    17701 atataaataa aaatagctta gggacatata taatagatag ttatataaca cagatgttaa
    17761 aaggaaatat agagagaaaa agtagtgaga atggtacaga agtattaatt actataccta
    17821 cacaaattaa tatttaaata ataactcatc tacaaagtag tagttttaat tgagcaaaga
    17881 ggcttaaggt tattaataga ttaagttaat tgaatgtatt tataaaaatt ataagtatat
    17941 attcaattta acaggtattt attataactt taagcctctt tgcttgttta agacatactt
    18001 aaattttagg aggtgagaaa atgagagaag agttattaag tgttggtatt gacattggaa
    18061 caagtactac tcagttaatt ttttctaaat taatagtaga aaatactgaa tcaaattttt
    18121 cggtacctag aataagtata gtagacaaag aaattattta taaaagcaaa gtttatttca
    18181 cccctcttat ttcaaataat gaaataaatg gtcaacaaat aaaagatata gttgaaaatg
    18241 aatataaaaa agcaaatata tctaaagaag atatacatac aggagcagta ataataactg
    18301 gtgaaacagc tagaaaagaa aatgcaaatg atgtacttca tactttgagt ggatttgctg
    18361 gggacttcgt tgttgcaaca gcaggacctg atttagaaag tataatatca ggaaaaggag
    18421 ctggagctca tatttattca aaagaacatt caactacaat tgtaaatata gacataggtg
    18481 ggggaactag taacatagcc tcatttaaaa ggggagaagt aaccgatact ggatgtttgg
    18541 atataggtgg aaggcttgta aagatagata aaataagtaa aaaaataact tatatatcac
    18601 ctaaaataga taaaatcata aaagataata atttaaatct tcaagtaggc acaatggcta
    18661 ctgtagaaaa tacaaagcca atagtggata taatggttga cttattaatg gaaagcatag
    18721 gattaaagaa acagacacct ttgtttgatt ttataataac caataaatca ataagtatag
    18781 atgaaactat taaaaatgtt tcattctctg gtggagtagc tgattatatt tactatgatg
    18841 gagaaataaa agattatttt gaatatggtg acttgggaat tttacttggg caagcaataa
    18901 aaaattgtga ataccttaaa aaagtaaatg tagttaaatc tatggaaaca ataagagcca
    18961 cagtggttgg agcaggttct catactactg aagtaagtgg aagtacaata acttacacta
    19021 gcgaaaagtt tccacttaag aacttgccag ttttaaaatt atcaattgat gaagaaacag
    19081 gtacttcaaa acaaatcagt gaagctataa aaaataaact taattggttt aaactagaaa
    19141 atgatttaca gaaagttgct atagccatga taggaaagaa aaatccaacc tttttagata
    19201 tacaagagta tgctaaaggg tttgtagatg gaatgcaaca aatcatagaa aatgatttac
    19261 cttttatagt aattgttgaa aatgatatgg caaaagtgtt aggtcaagca atatatgcat
    19321 tattaaatta tgaaaaagaa attgtttgta tagacagtgt caaggttgaa aatggagact
    19381 atatcgacat tggaaaacct atagttgaag gtaaagtttt acctgtggta ataaaaacat
    19441 tagtttttaa ttaatagaag gaggtaagta aatgatttta aatacaaaat tatttggacg
    19501 tacttatcag tttaaaggct tatatgatgt aatggctaaa gctaatgaag aaaaatcagg
    19561 agataaatta gcaggtcttg cagcagaatc agctgaagaa agaatagcag caaaagttgt
    19621 tttatcacac ataaaattgg aggatataag aaataatcct gcagtggcat atgaagaaga
    19681 tgaagtaaca agaataatac aagatgctgt aaatgaaaaa gtttatgaag aaataaaaaa
    19741 ttggacagtc tctgaactta gagaatggct attggattca aatactacaa gtcttgatat
    19801 aagaagaata tcaagaggat taacttctga aatgatagca gcagttgcaa aattaatgtc
    19861 aaatatggac ttagtttatg cagctaaaaa gataaaggtt actgctcact gtaatacaac
    19921 tataggagaa caaggaacat tatcagcaag acttcaacct aatcatccaa cagacgaccc
    19981 agatggaata cttgcatcac ttcttgaagg tttaactttt ggtataggag atgcagtact
    20041 tggattaaat ccagttgatg actctgttga gagtgttact aaagtattaa aaagatttga
    20101 agaagtaaaa caaaaatatc aaataccaac tcaaacatgt gtattggcac acgttactac
    20161 acaaatggaa gcaataagaa atggtgctcc tgcagattta atattccaat ctatagcagg
    20221 ttctgaaaaa ggaaatgaag catttggatt taacggcgac actatagaag aagctagaca
    20281 gttagcttta aaacacggta cagctacagg acctaacgtt atgtattttg aaactggaca
    20341 aggttcagaa ttatcttcag aagcacatca tggaatagac caagttacta tggaagctag
    20401 atgctatgga tttgctaaaa agtatgaacc attcttagta aatacagttg ttgggtttat
    20461 aggacctgag tacttatatg atgctagaga agttgtaaga gcaggacttg aagaccactt
    20521 tatgggtaaa ctacatggct taccaatggg tgttgatgtt tgttacacta accatatgaa
    20581 ggctgaccaa aatgatatgg aaaacttagc catactatta acaacagcag gatgtacgta
    20641 ttttatggga atacctcatg gagatgatgt tatgcttaac tatcaaacaa caggatacca
    20701 tgaaacagca tcacttagag aaatgtttgg actaacagca ataaaagaat ttgatgcttg
    20761 gatggaaaag atgggattct ctaaagatgg taagttaaca gagttggcgg gagacgcatc
    20821 tgtattatta ggataggagg tatttaatat gaatgagaag gatttaaaag cattggtaga
    20881 acaattagtt ggacaaatgg ttggagaatt agatactaat gttgtgtctg agactgttaa
    20941 aaaggcaact gaagttgtag tagacaacaa tgcttatata gatgatataa cagaagttga
    21001 tataagaaag caattactag taaaaaatcc taaggatgca gaagcttatt tagatatgaa
    21061 agcaaaaaca cctgcaagat taggtatagg aagagctggt actagatata aaacagaaac
    21121 agttttaaga tttagagcag accatgctgc tgcacaagat gctgtattct cttatgtaga
    21181 tgaagaattt ataaaaggaa ataatatgtt tgcagtggaa actttatgta aagataaaga
    21241 tgaatactta acaagaccag atttgggaag aaaattttct ccagaaacta taaataatat
    21301 aaaatctaaa tttggtacaa atcaaaaggt tttaatatta gttggagatg ggcttagctc
    21361 agctgcaata gaggcaaatt taaaagactg tgtgccagct ataaaacagg gtctaaaaat
    21421 gtatggtata gattcaagtg aaattttatt tgttaaacat tgtagagttg gtgctatgga
    21481 ccacttaggt gaagaattag gttgtgaagt tatatgtatg ttggttggag aaagaccagg
    21541 attagttact gctgaatcaa tgtcagcata tatagcttat aagccatata taggaatggc
    21601 agaagcaaaa agaacagtta tatcaaatat tcacaaaggt ggaacaactg cagttgaagc
    21661 aggtgctcat atagctgaac ttataaaaac tatgttagat aaaaaagctt caggaataga
    21721 ccttaaataa agtcgaggga gtgtagaata tgaaaaatga tgtaattcgt cctaatatac
    21781 taggaattaa aataatttca aatataagtc cagaaatggc acaaaaacta gaattaaaat
    21841 cacatcacaa aagcttaggt ttaataactg cagactgtga tgatgttacg tatacagctc
    21901 ttgacgaagc aactaaagct tcagaagttg atgttgtata tgcaaaatca atgtatgcag
    21961 gtgctgcaaa tgcttcaaca aaacttgctg gtgaagtatt aggaataata gcaggtccaa
    22021 gcccagcaga agtaagaagt ggtctaaatg cagtagttga ttttctagag tatggagcaa
    22081 cttttataag tgcaaatgat gatgattcaa tagcatacta tgctcactgc gtatcaagaa
    22141 caggtacata cctttcagag gtagcaggaa taagagaagg tgaagcatta gcttatttag
    22201 tagcaccacc ccttgaagca atgtatgcac tagatgcagc aatgaaagct gcagatgtta
    22261 agatgtgtga attatttgca cctccaactg aaacaaactt tggtggagca ttacttacag
    22321 gttctcaatc ggcttgtaaa gcagcatgtg atgcatttgc agaagcagta aaatcagttg
    22381 cagacaatcc aacaggattt taaggatagt tagatggatg ctataggctt aatagaagta
    22441 ataggatatg ttgctgcaat agaagcttct gatgcttgtt taaaatctgc aaatgttaat
    22501 attgtaagag ttgataaggt tggggcaggt attgttactt taacaatatg tggagatgtc
    22561 ggtgctgtaa aagctgcttt agaagcaggt gaaatagcag cttccagagt tggtacatta
    22621 agaacatcac atattatacc aagaatccat aatgaagtaa caaatgcttt attcaaaact
    22681 aaagaaccta aagtctgtga agtttcagaa gataaagaaa aaaactttga gattacattg
    22741 gaaataactg ataatgtatc gaaaaattta tcagaaaaag aaaatgtaat tttagatgtc
    22801 aatgaaaata gcggaaatac tgaaaattta tatagtgata tagataaaac aaacaaaaaa
    22861 attgaaacag ttgatgatag tttaacagaa gctaatgagg aaaaaataga aaacactaat
    22921 gataaagaag tattggaaca agttaaaata gaaaattccg attttgatag agatgaccta
    22981 tcaaagaaca gtgtaaaaaa acttaaagct atggctaaga aattagattc ttcaataact
    23041 tataaagatt taaatgttct taaaaaagaa gaattaataa acttagttaa aaagcttagc
    23101 agaagggata gatagttatg acacttttag ataaagattt agtatctata caagaagtca
    23161 gaactcttgt aagaagatct aaagtggctc aagagaaact ttctaaaatg agtcaagagc
    23221 aaatagataa aatagtaaaa tctgttgtag atgcagcatt taataatgct actaaattag
    23281 ctaaaatggc aaatgaagaa actggatttg gaaaatggga agataaagta attaaaaata
    23341 cttttgcatc tatgggagtt tatgaaactt taaagaacac aaaaactatt ggaataattg
    23401 gtgaagataa agaagccaat acgatagata tagctacacc agttggagtt gttgcaggtc
    23461 ttataccttc aacaaaccca acatcaacag taatatacaa aactataata tctctaaaag
    23521 caggaaatac tatagttttc tctccacatc caaatgcaaa aaactgcata cttgaaacag
    23581 ttaatataat aagtgaggct gctataaagg ctggagctcc agaggattca atattatgta
    23641 taactaatcc aagtatagtt ggaaccaatg aattgatgaa acataaagat acaaacttaa
    23701 tactagcaac tggaggagaa gcaatggtta gagctgctta ctcttcagga acaccagcta
    23761 ttggagttgg accaggaaat ggaccagcat ttatagataa atcagcaaat gttaaattag
    23821 caatcaaaag aataatagat tctaaaactt ttgataatgg tgttatatgt gcatcagaac
    23881 aatctatagt aatagaaaaa tctatggaag aaactgtttt aaaagagctt aaaaatcaag
    23941 gtgcatatgt tttaaatgaa gaggaaagtg aaagattagc aaaattcata ttaagagcta
    24001 atggtactat gaatccacaa atagttggta aaagtgttga ggtaatagca aatttagcag
    24061 acttaacaaa tgtacctaaa aatacaaaag ttctagttgc aagagaaaat aaagttggtc
    24121 acaatgtagc ctattcaaga gaaaaactta caccaatact agcgttctat gttgaagaaa
    24181 atgtagaagc tattatgaac agatgcagag aaatattgtt aaatgaaggt gcaggtcata
    24241 cattctcaat gcatgtaaat gatgaagaat tggtaaaaag atttgccctt aatatgccag
    24301 tatcaagaat acttgtgaat actccttcag cacttggagg aataggtgca tcaacaaacc
    24361 ttgtaccagc actaacactt ggttgtggtg caataggtgg aagttcaact tcaaacaata
    24421 taggaccact tgatttaata aatgttaaaa gagtagctta tggaatcaaa gaaatagaag
    24481 aacttggaag agataaatct caagttgaaa aatcaatgtt tgactcaatg gataaagaag
    24541 aattaataaa tcttcttgtt caaaaactag tcaaagaact agcataacta aaataaacaa
    24601 tgaaacttat agctatcact ataaatttct aaaaatataa actataaaaa actataaaat
    24661 taaatttttg gaggataaga ttatggcaag tgcaaacgca ttaggaatga tagaaactaa
    24721 aggattagta ggagcaatag aagcagcaga tgcaatggtt aaagctgcaa atgtacaatt
    24781 agtaggaaaa gagcaagtag gtggaggatt agttactgta atggtaagag gagatgttgg
    24841 agctgttaaa gctgctacag atgcaggagc tgctgctgcc gagagagttg gagaactaat
    24901 atcagttcat gttataccaa gaccacactt tgaagtagat gctatattac caaaggtatc
    24961 agctgagtaa tataagaata attataaaat ttaaattttg attgaaaaag aggctatctc
    25021 aaaaatgagt cttactaata aactcaatgt attttgaggt agtctttttt atacatctaa
    25081 taggagatgt gataaaatgg gtgttttaac agaaagcgaa gtaaaaagaa aaattggaaa
    25141 aaacaaaaat aatataactg aattttatat agaaaaaggt caaataataa ctccttctgc
    25201 aaaatcttat ttagctgaga gaagtattga gcttaaatat gttgataaac tttcagatgt
    25261 aaacaatgaa cctaaagaag ttgtaaaaga agtagttaaa gagaaaaaat atagatatac
    25321 tactgtattt ggcgctaaat tggatgaaaa accagaacat atgactcaat taaatggaaa
    25381 tcttttagtt tttaaagacc ataaaagaat aatattgaga ggaaaaatag attctcttga
    25441 atcagaaata ctaaaggtac agatactttg tcagaaacta gatatgccaa agcttgttaa
    25501 ggaattgcaa gaagtattag actttgtaag aaatataatg agatgtgaag ttttagatga
    25561 aaagctaggg gactttaaac ttttaggatt aactcctcaa gaacttagag aacaatctca
    25621 ctatcctaaa aaatactttg gtataggaca tgagttccca agttatgaaa tgggagaagt
    25681 agtagtagct ataaatagta taagaagtaa tacacgagag gttgaacttg cagcctatga
    25741 agcatttaag ggtgagtatg gaaatgttca cagagaagat ttaataaagg cacttaatag
    25801 attatcgtca gtattttgga taatgatata taaggttaga actaataagt ataagtagtt
    25861 ggaggacata atggagaatc tattaaatga aatagtggaa gaagtaataa ctagagttaa
    25921 aaaagaagca tttatagaag ttgaagcatc aggaagacac gttcacttaa gtagagaaga
    25981 tgtcgataaa ttatttggag aaggatatac tcttacaaaa ctaaaagacc tatctcaacc
    26041 aggtcaatat gcatgtaagg aaagagttac aataactggt cctaaaggaa gtataaaaaa
    26101 tgtagttgta cttggaccat gtagaaatga aactcaggta gaaatatctt taacagatgg
    26161 ttcaactctt ggactaaaag ctcctataaa acaaagtgga gacttagaag gtactttaag
    26221 tattaaaata tcaacacaat atggtgaagt tgaacttgat aaggggttaa tggttgctaa
    26281 aagacatata cacatgactc ctaaagatgc tgaaaaattc aatgtatgtg ataaagagat
    26341 agttgaagct aaggtgatgg gtcaaagacc tttaacattt gatgatgtag taataagagt
    26401 gagtgacagt tttaaaactt atatgcatat agactatgat gaagccaatg catgtggtta
    26461 ttcaaaagga actgttgcta aaataataaa gaaagcgtaa atttaaccta ctatataaat
    26521 tacctctagg gaggatttaa tatgaattat gataaccttg tagatttaat aacggaagaa
    26581 atctataaaa aaataaatag taatgaaata aaaatatcta ataaacctaa agctgtgatt
    26641 gtatttgaac aagataacaa taaatttaac ttattaaaag atgaatttga aatagtagaa
    26701 tttgataaaa gtataaggga atgtgaaata gtgatagttt caagactttg tatgaggggg
    26761 ctatcgaata tagcacttgg aaattcaaca agtgatgaag aaagatttat actaaaaatg
    26821 attatgaagg gcaaagaagt atatgtccta gatgaaggaa ttgaatacaa aaaatataag
    26881 gatactgcac caaaagcatt atataagaaa tttatgtcat ttgaagatga aatatgtaaa
    26941 tttggtgtag aaattataaa agacttaaat gttataacta aaaataagtt aaatataaag
    27001 aaagaactag aaagtactag aggttctaaa aaagaagata caaaagtttt agataaagat
    27061 gttttaaatt taatcagtgt agaaagtgta aaaatagata atgaatttag tttagattta
    27121 aggaataaaa aacttatatc ggaagctgac ttaagaaaac caactataaa tggagttaaa
    27181 aacatattag ttaataaaaa aagtataata acaccattag ctgtagattt tatgagaata
    27241 caccatctca aactaaagaa agtgtaagga ggtaatttta tggatatagg aaaagttgta
    27301 ggtaatgttt gggctacaag aaaagatgaa aaattatgtg gccaaaaact tttagttgta
    27361 aaaattttaa aaactaaaga cacatacaag gaaggcttca tggttgcagc cgataatgca
    27421 ggagcaggaa atggagactt agttcttata tccaaaggtt cctcagctag agagtcgatt
    27481 gaaaaatctc atgttccggt agatgcaact atagttggag tagtcgattc gtttgaggtg
    27541 tttgatgaat aaaagtatag gagctataga gtttaaaagc attgctaaag gtatagaagt
    27601 atccaatgaa atgataaaga aatcttcagt tgatgtattg tacttaaaaa gtatttgccc
    27661 aggtaaattt ctaataatag taggtggaga aacttcttat ataaatgaat gtgtagatta
    27721 tggaataaaa cttggagaag gatatattgt agataacttc gtaataaatg caatttcaca
    27781 agaaatatta gatggattta aaaataagta tcaaaagcta gatagtatag tctctatagg
    27841 cgttgttgaa agtagtaaag tatgcgctgg aataaaaatg cttgataaga ctttaaaatc
    27901 tggcgactta gtattagtaa aacttcaatt atcttttgct ataggtggca aattagtata
    27961 tatagtagca ggagatttaa gtagtttaga atatgcatta aaagaaagtg aaaatgtagt
    28021 aagagaaaaa gaagtaatat ataagactgt aataccatca gtagacagtc aaataataaa
    28081 aagtctaata aaataatggg gggaattatg agtataaacg aaattataat ctatgtaatg
    28141 gtagtcttca tggtacttgg agctatagat aaatgtatag gtaacaaatt tggtcttgga
    28201 gaacaatttg aagaaggtat aatggcaatg gggtcacttg cagttgcaat ggttggtgtt
    28261 atatgtttag caccagttct tgctgatgta ttaagaccaa ttgtagtacc agtatttaat
    28321 atacttggag ctgacccagc tatgtttgct ggaagtttat tagctaatga tatgggtggt
    28381 gcaccacttg cacttgaact tgcgaaagac cctaatgctg ggttatttgg tggattaata
    28441 gtaggggcta tgatgggacc tacaatagta tttataatac cagttgcgct tggaataatt
    28501 gaaaaagaag accaaaaatt cttagcaaca ggaatacttg caggtataat aacaatacct
    28561 attggagcat tcgttggtgg attagttgca ggattcgagg taatgatggt tcttagaaac
    28621 ttagtgccaa taataatatt tgcagtagtt atagcacttg gattattaaa attcgaaaat
    28681 gctatgataa aaggattcac atactttgga aaaggtgtag taatagttat aactttagga
    28741 cttgctgctg ccatagttga agctttaaca ggaatagttg taatacctgg aatgaaacca
    28801 atcagtgaag gtatagaaat agttggagat atagcaatag ttttagcagg agcattccca
    28861 ttagtgtttg ttataacaaa ggtatttaat aaaccattaa tggctttagg aaaagttctt
    28921 ggaatgaatg atgtatcagc agcaggcctt gttgcaagtt tagctaactg tataccaatg
    28981 tttggtatga tgaaagatat ggacaataga ggaaaaataa taaatgtagc attctctgta
    29041 tcagcagcat tcgtatttgg agaccactta ggatttacgg caggatttaa ttctaatatg
    29101 ataacaccaa tgatagttgc taaattagtt ggtggtataa cagcagtatt gttagctatg
    29161 gttatcgcaa ataagacact aaagaaggag aatgtataag atggatatat caaatataga
    29221 taaaaattta attgaaacat tagtaagaca aataatagaa gaaaaaataa gtggaactaa
    29281 agacacagta gattttgtta gaaataaaga cattagtgga ataacttcta taaagttacc
    29341 aactgtaaaa gttagtgaat ctgatagatt agatacaggg aatccaagtg atgtagttta
    29401 cacaaaagat ttatttacat tagaggaaag cccaagatta ggttgtggta tgatggaaat
    29461 gaaagaaaca acttttgatt ggactctaaa ctatgatgaa atagattatg taatagatgg
    29521 aactttagat ataataatag atggaagaaa ggtttctgct tcttcaggtg aattaatatt
    29581 tataccaaaa ggaagtaaga tacaattttc tgtacctgat tatgctagat ttatatatgt
    29641 aacatatccg gcagattggg catctcaaaa ttaataaaaa tcaagttaag ttaataataa
    29701 tcaaataaat aatttaaaat acaaactctt aaataagcac acatatttaa aaaagctatg
    29761 aattaaaatt catagctttt tttataaaga atgttatatt ttagacgtat tttttctata
    29821 tttactggga gacatattat attttttatt aaataatcta ataaaatgct ctacatttgg
    29881 ataaccaata ctattttact gataatcttt tccttatcac taatcttaca ttttaaaata
    29941 ttatttttac tcgtaatatt atcataagaa tttgtttctt atttaaaact aattagaatt
    30001 ataaagttta ttgccatgtt attaataaaa taaatccagt taaatcgagt gctagttaaa
    30061 gtaataagaa acttttacaa tggtttcctt tatcgattga aataaaagtt aatatggtat
    30121 aatatctaaa gagagaatct tatcaattag aatatgcata gaaacaagtg gcttaaaagg
    30181 agacgttata tggaagagat agtaaagaag gaacaagcaa gtatgaatga tatttttttg
    30241 tatcttaaag ctaaatcaaa ctcattatat aaatttgtaa tattatataa tgagtttatc
    30301 aataaaccta aagattatgg aactggggaa cttataaaca tggttgaagt acatatgcta
    30361 acagcgattg aagaaaatcc aggaataagt ccaactcagt tagctaaaat atggaacaga
    30421 actaaaggtg caatatctca aactatatct aaattggaga aaaagggtta tataactaaa
    30481 gaaaagaaag aaggaaatgc aaaaactata ttactatatc caaccacatc aggagtagag
    30541 ttaagtaagg cacataaact atatgacacc atagatgtta caagaacatt tgaatactta
    30601 atcaaggagt gtacattaga ggagatagat agtttttata aagtaattga tttgtttatt
    30661 aatttattag aagaatctcc gcaggaaaat aatgattctg aaaaagtgta gctgagattg
    30721 aagttgaata aagagaaata gatattatag aagtagtact taagtacaca ttatataaaa
    30781 atataatagg tataggatta aaaagtttag tggtttaaag tttaaccact aaactttttt
    30841 aatgtctata atctactatt atttaattta ctaaaaataa aaaaactcat tatattaaga
    30901 taaaaataga aaaataacta gaaaaatact ataatatatc agcttaatac agaaaaaagt
    30961 taaaaaattt gagaaaaata aaaatgaaaa cgttttatat tgacatagag aaaaagtagt
    31021 gataccattt aaatatagtt tagtagctaa actatattta aattataata actattttag
    31081 ggggatgtta tgaatttaaa acaattaagt gaaaaatacg aagaatatgt aattcaaatg
    31141 agaagagaat ttcataagta tccagaatta tcttttaaag aaataagaac aacccaaaga
    31201 ataagggaag aattacaaaa aataggggtc ccttttgtat ctattgaacc aaatatcgta
    31261 atagctacta tacaaggcaa aaatgaaggt aagacaattg caattagagg agatatagat
    31321 gcgcttccaa tgcaagaaga ttcagatgtt gaatacaaat cagaatatga tggaataatg
    31381 cattcttgtg gacatgatgc tcatggagct atgttactag gacttgcaca tatgctaaat
    31441 gatataagaa aagatttaaa aggtagagta ttattagttt ttcaagcagc tgaagaagtt
    31501 ggtggtggac acaaggagat aatacagtac tttaaggaaa atggaggacc agatagatta
    31561 atagctactc atgtttggtc cgatattgaa gctggtaaaa tttctgttga accaggacct
    31621 cgtatggctg gtgggtctgg ttggagaata gacataacag gtagaggagg tcatggttct
    31681 agaccagatc aaagcataga tcctataaag ccagcttgtg atatagtttt aaaagtatct
    31741 tctataccag ttaatcatat aagtgtacta gaacaatgtg ttgtacatgc atgtgcaata
    31801 catggaggaa ctacaatagg aaatgttatt ccaaacaatg cagaggtaat aggtggatat
    31861 agatatttta ctgaagaatc aggcaaaaaa gtgtttgaca ttataaagca aatgagtaat
    31921 ggtgttggac aagtttatgg agtggatgtg aaggttactc atactggagg cataggacct
    31981 gttatcaatg atgaagaagc agttaagatg gctaaagaaa tagtttctaa tatagatgga
    32041 ttggagttag attcttatga gccaatatgt gcatcagatt gttatggtga aatattaaaa
    32101 gagtattctg gcttttattg ttatctaggg gtaggtaaca aagaaaaagg aataatatat
    32161 gcacaacacc atcctaagta caatatagat gaagatacat taaagttagg ttgcgaattt
    32221 atggctaaat atgcagttga atttttaaat aaaaataata actaaaataa atcgaatttt
    32281 ataaatgggg gggatgaaaa tgcaaggtgc attaaaggaa aataagaaaa aaagtggtgg
    32341 aggattttta ggatggattg aaaaagttgg gaataaaatg ccacatcctc tagcattatt
    32401 tacatatatt acattgatag tagtagcatt atctgcaata gcaagctttt taggtttttc
    32461 agcaacaagt cctgctacag gaaagttaat aaaagttatg aacttaataa gtgcaaaagg
    32521 tttagtacta tttatgcaag agtttgttaa gaacttccaa aacttccctg tgcttggagt
    32581 agttttagtt atggcagttg ccacaggagt ttgtgaaaag acaggatttt tttcaactgc
    32641 cataaaaatg agtttatcaa aagttaaggg gaatgcggtt gtatttataa tagcttttat
    32701 aggagtattt gctaatcaag ctggtgatgc tgcttttgta ttagtaccaa ctcttgcagg
    32761 agcaatattt tatggattaa atagaaatcc attagctggt atattctgtg gatttgcatc
    32821 agttggtggt ggatttgcaa cagctgtaat tcctggggga tgggacgtta cgctaacgcc
    32881 aataactgta tcagctgctc aaactattca acctgcattt gatatgacat tattagctag
    32941 ttattatgca ctatttgtat ctgcgtttat agtagctaca gtatcaacaa tagtaacggt
    33001 taaatttata gaacctaaat taggtaagta tactggtaaa ccagaaggta tagatgataa
    33061 tcaaggtttt gaggtcacaa aagaacaagc aaaagcagca aagttagcag gttttacagt
    33121 tttagcttat gtagcgttat taattgcttt gtgtatacca gctaatagtt ttttaagaag
    33181 tccagaaggt tctttaatat ttggagcacc acttatgtct tgcttacagt tttttatagt
    33241 aatactattc ttcttgccag ggattgtgta tggaatgagg gttggtaaaa tcaagactgt
    33301 aaaagactta aatgatatat tatgtcaatc aatggcagca atggcaccgt ttatcgtact
    33361 tgcaatagtt ataggtcaat tcttaacgct attctcagtg tctaatttag cacaggtact
    33421 agctataaaa ggtggagcat tactcaactc attgccacta ccaccacaag ctataatact
    33481 gctattctta gtactagtag cgtttataaa tatatttgtt ataagcggaa gtacaaaatg
    33541 gatgatattt ggaccagtat ttgttccaat gttaatgcaa ttaaacatac atccagcatt
    33601 tactcagttt gtatatagat taggagattc tgtaaccaat catttatcac cattaaatgc
    33661 attctttata atacttttag caacagtcca aaaatacgat aaaaatgcag gtatgggaac
    33721 tatattctca gctatgatac cttacactat aggttacttt gtagtacttg cagttttagt
    33781 agtagtttgg atgacaatag gtataccagt aggaataggt ggacaagttt ggttataaaa
    33841 catatttaat aattttggga ggaaagaaaa tggaaacaac tgaaataaga tacaattttg
    33901 atgaagtaat agatagaagt ggaacaaact gtcttaaata tgatcttaag agtcaattta
    33961 tgccagaatc tccagaagat gcactaccac tatgggtagc agatatggat tttgcatgtg
    34021 cccctgagat aattaatgca atgcatgaaa gaatagacag aaaaatattt ggatatagct
    34081 ctcaacaaac tgatgattat tatgaagctg tatgtggatg gtttaagaga agatttgatt
    34141 ggaaaataga aaaagagaat atattcttta gcccaggggt tgtgcctgcg ttaggatttt
    34201 tcacacaaat attaacacaa aagggtgatg gtatcataat agaaaagcct gtatactatc
    34261 cttttgaaga taaaataact aataatgaaa gaaaagtagt taatagtcct ttaaagtttg
    34321 aagatggtca ttattctatg gattatgatg gatttgaaga attagctaaa gatgaaaata
    34381 ataaggtgtt tatattatgt agtcctcata atccagttgg tagagtatgg aaaaaagaag
    34441 agcttagaaa aatagtagac atatgtaaga aatatgattt gtggataata tctgatgaaa
    34501 tacattgtga tattattcgt aaaggtgaaa agcatatacc attagaagta gcatgtcctg
    34561 attataaaga tagaataata acttgtacag ctccaagtaa gagttttaac ttagctggta
    34621 tgcaattatc aaatatagta atcaataata aggaattaca aaaaaaatgg atgcttgaaa
    34681 cagattctaa attagctata ttcctaccta atccattcgc aatagttgct actaaagttg
    34741 cttataatga ttgtgaagat tggatgaatc aagtaaatga gtatatagat ggtaatatta
    34801 aatatgtaga agattttgtt aatgagtata tgcctaaagt aaagataata aaatctcaag
    34861 gaacttactt agtttggtta gactttagag ggtatggatt agatgcaaaa cagcttgaat
    34921 atgtaatgct taacaaggcg aaggtattgc tagatgaagg atacatattc ggagaagaag
    34981 gagcaggatt tgaaagaata aatgttgctt cacctagaac aattatagaa gagtgtatga
    35041 atagaataaa atgtgcattt gaaggattat aatagtaata tctaaataat atatttgtgt
    35101 attttaaaaa aagactatgc taaaaaaata tttcaaagca tagtctttta aagttttatg
    35161 atgagtttaa aaaatttcaa aacgttacta ttcacttata tgtttatatt agaaataatt
    35221 ccacaatata ctcattttct aaagttgcac ttttatacac cttgcttcac caatatcatg
    35281 tgtaaccaaa atagtagact tattctcatt cttaataata ctataaacat catcacaaac
    35341 taataatcta gtctgataat caagagctga aaaaggttca tctaaaagta gaatatctgg
    35401 atttacagaa agtgttctaa taagcgcaac tctttgtctc atacctccag ataattcttt
    35461 tggatacata cttctaaaat cccataatcc ataagtcttt aaaaggcttt caactctatc
    35521 taaagatttt ttatcttttt tgccttgaat ttccaatcca atagttatat tatccattat
    35581 atttctccat tcaagcaaat tatctttttg aaacatatat ccaatatttc cagtaatctt
    35641 tatatcacca ctagtcggtt ttaaaagatt agtaagaata tttaatattg tagatttacc
    35701 acttccagaa ggaccaagca aagttaatat ttctccttct ttcaagttaa aattgacatc
    35761 ctttaatact tcaagctcgc cttccttagt gtagaagttc atatttatat ttttaacttc
    35821 tactatgttg tcattcatta tgccaccccc tataactttt agctcgtata tattatgtta
    35881 ttaacaatat gacctatttg taccattttt cataaatata atataaatat tttgtttatt
    35941 tacgtatttt cttagtaact tgtacgtata aattatagat aaataaatta taaggtaggg
    36001 gataaatttg agtaacataa taacgaattt aatagagtca atatttacta atccaataac
    36061 tatattgttt ttacttgcaa taactgtgta ttctgttttt aaaataatag aaaataacag
    36121 agtatattca tttgtaagtt caaaattaga cgaaatcaat agagactata agacaagtga
    36181 gttttataaa aaggtaaggg atgactacta tacatatagt aaagaaaatc cttatgcaga
    36241 cgtaaatata acttcattta tagaggaagt tgtatctgat ttaaagcata ataatttgcc
    36301 attgttagaa aaaattagaa gtataaaaaa ttcatcttca ataagtatat tacttggggt
    36361 tttaggtacg tttgttggtc tttctacaat gcttttatgt gttgatacta aagacattat
    36421 aaattcactt ccttcaacta ttagtagtat gcaaactgca tttacaacta gtatatttgg
    36481 agttgtattt tctctaatta taggttactt tactaagata aaagattgtg aacatgtttt
    36541 gatacaaata atgttaaaat ctgaaaattt actaacatct gaaataactc attttaagtc
    36601 agaaagaatg gatttaaaag tagaagaggt aaaaaataca ataaaacaaa taagtaagtc
    36661 tatagaagct attgaaaaat ttgataaaat atctaaagat ttaaatgact ttaatgatga
    36721 atttatcagt ggtatagaag ctttaaaaag ccttttagaa ggttctcaaa gttctattaa
    36781 gacatttgac cagagcgtaa gaaaattaga taaacagttt aatattttaa atataaaatt
    36841 tgtaaagctt tttgataaat atgataatca agataatata aataaagaga ttttatttga
    36901 tataaaagaa tcttcaaaaa atatatacaa tgccactgaa agccaattca aaattagaga
    36961 ttatataaaa aatataaatg caggatttgc tctatatgaa agaagtgctc aagatttatt
    37021 aactaaatta atgacccatg aaaataaaat atctcaaaat caaaaaatcc ttttagatga
    37081 aaagtttacc ttagatgact caataaaaaa tctctcaagt attattgaaa acttttctaa
    37141 tgacctacaa gttaaactgg atatgatgtt tgaaaattct ttagatattc aagataagtt
    37201 agatgttatg tttaataact cttttatgaa tgatgaagtt cctcttgata gtgaagaatt
    37261 atttaaggat gatgttaata atgtatttaa tccttttagt gaagaaattt atgagataga
    37321 ggataaagaa ataaaggtca taggtgagga tgagttaaat gaataataaa tatagaagaa
    37381 ctgttaacag agaatttgaa aagagtagct tttggccaac atttacagat ttattatcta
    37441 ctgtattgat ggttgtgatt cttatcttat ttagctcaga aagtatttca ggttctgttg
    37501 aacaagattt agctaagaat gtcaatgctt ctgttgaaga aacttttaaa aaaagtggaa
    37561 tacctgtgaa ggtagataaa tctaatgggc aagtaacttt tggtgaaaaa accatgtttg
    37621 atgtagatag tgatgtttta aaaccagagg ctaaagaaat gctaaaaatg tttgttccaa
    37681 agtacataga gactatttat aaagattatg gtgactatat atccaaaata gttatagaag
    37741 gtcatacaga tgatgtggga agttatatat acaacttaga tttatcgcaa agaagagcat
    37801 atagcgttgc taaatttata gtgggagatg aaataggtga ctataaatac aaagataaag
    37861 tgactaagca tattattgca ataggaaggt caaaagctga actcatcaaa aatggtgaca
    37921 atagcgtgaa tagagatgca tctagaagag tagaactaaa atacgaaata aatattaatc
    37981 aaaataaata attaaatttt tataaaaaaa aagaaggcag ttggtgtgag ctttaagcct
    38041 tctttaagga aaaagtttat ttatttttgt tgatacttaa catattatag tttttagaag
    38101 cagtttgcaa tatattaaac gtaaatggtc gtttaaaatg cataactgga tactactgat
    38161 gtaaaatatt tcattttata taatataaaa attatacagt gtcaattttc tatattattg
    38221 tactataatc tattatttaa aatattatgt aaaaaaatat cataaaaacg catatcatgt
    38281 tataattact agagtattat gaatatggag gcgtagtttg tgatagaaat aagtataaaa
    38341 aataaaaaaa attctaaaat taaaactttg atacttttat tgatagtatc tatatcgatt
    38401 tatttaactg gttgtactac atcagataat ggaaataatc aatcaaataa taatgattct
    38461 ttgtctagta gtatatcaag tgataagaat gtaaaaattc attttattga cactggaaat
    38521 agtgatgcaa tattgattca agatggtaaa acttttacat tgatagatgg gggagataat
    38581 gatgatgagg gattaatggt agattaccta aacaatcaag gtgtaaaaga tattaaatat
    38641 cttatagcta cccattctca tgcagaccat ttaggtggac ttgattctgt tgtcaaaaac
    38701 tttaatatag aaaatgtttt tgtatcgaat ggttcagcag aaacaaagag ttatagggac
    38761 tttataaatg ctttagcaaa taagggttta tccccaagtg taccgcttga aaataataag
    38821 ttctatttag aagattctta ttttgaagtt ctaaatacaa atgggggaga tactactaat
    38881 gaacaatctc tagtccttgt atatactaat gggaatgata aagttttatt tacaggtgat
    38941 gctgaagaag gtaccgaaaa agaaatacta cctaagcttg agaaagtaga ccttttaaaa
    39001 gtagctcatc atggttctcg tagtagttca tctcaagagt ttttagataa agtaaatcct
    39061 gaatatgcgg ttcttttagt aggcaagggt aattcatatg gtcatccaca tcaggagaca
    39121 atgaataaat taaaaaaaat gggagtaaaa gtgcatagaa gtgatgagtg ttcagatata
    39181 atatttgaat caacaggtga tggagtattt acaagttgta aagatggaag ttataataaa
    39241 ggcgttagag aagatggaaa ctctggaaaa tcaaacagta atacaagtaa aaatgattct
    39301 tttaaaaata aacaaaatac atccaattca gaagaaatag tgtattttac tccaaaaggt
    39361 aagagctatc actctacaaa aaattgtagt gggctttcaa gaagtaaaaa aatattatca
    39421 ggcacaattg cagaaagtaa gaaaaatgac ccatgtgata tatgttatgg aaaataacaa
    39481 aaaaacaacc aatttatcta taaagcttct gaactttaaa tcattcaaaa aataaaataa
    39541 atgcataaaa aaataagcta taatcaatag ataacacatt ttatctatac gaatagctta
    39601 tttttttaag tattttttaa tagctattta ataactctaa atacaccagt tacaatacct
    39661 acaatttcaa cttctgagtc attgagcata attggttcca taaaatcatt ttctggctga
    39721 agtcttatta aatttccttc tttaaaaaac ctcttaactg tagcagattc accatttata
    39781 agtgcaacta ctatctgaga atttaaggct gtattttttt tgtctactac aacgtaatct
    39841 tcatctaaaa ttcctgcatt aatcatactt tcgcctttta ctcttaatac aaaattgtta
    39901 ctgcccttta ctaaactggc agggaatgga atgtattcct ctatgttttg ttgagctaaa
    39961 atgggttctc cagctgttat ttgacctact aaaggaagct ctattatttc ttgattagct
    40021 ccagaaacat catttacttt actacgttct aaaacttcaa tagctcttgg tttagtaggg
    40081 tcctttctta tgtatcccag tttttcaagt ttatttagat gagagtgcac agttgaagta
    40141 gatcttaacc ctacagcagt acatatttct cttacagcag gtggataacc ttttaatatg
    40201 atttgagact ttataaattc caatatcaag acttgctttt cagttagatc taaatacata
    40261 acatcactca tttccaaaca tattttgttt ttatttccat acactttata tttttaatta
    40321 taacataatc catttatatc tgcaaacatt tgttcctgat ttttaatcgt cctcaatcca
    40381 ttcaaaattc ttgactatat cacgttttac atttttctta tgagctgcat aaagctgagt
    40441 agttgtgaca ttatcatggt caagtaagtt ttgaacatca tatatagaaa atcctgtttg
    40501 gattagagaa gttgcagccg ttgctcttaa tttatgggga ctatatccat tgtctctagt
    40561 agtatcaagt ggaatagatg tatatttttt taccagtgtt cttatagcct ttgctgtaat
    40621 tctcttattt tgaagagaca aaaataaagc atctttttct tcatcattta atagttcatc
    40681 tcttgttctt tcgttttgca aataatcttt tattacatgt tcacaagtat gatttatagg
    40741 cattaaaact tctttcccac gttttctata tattttaaat tcacctcttg aaaaattaaa
    40801 agaggagata ttgagttctc taagctcatt taatctaagc ccgtatgtaa caaataatgc
    40861 tagtatagct ttgtcacgta atttagtttt tctccaatat accttttctt tctctgtaag
    40921 cccttcacct gtttctactg attcaagcat cttagcaact tcatctatct caaggcgctt
    40981 aatagcatct ggctgaggtt taggaagttt tattggatta aatccatcag ttatattatt
    41041 atctatctga gagtttctgt ataagaattt aaatagagta gagatggatg atttttttct
    41101 tgctaaagct ctattgttat tttcaaaaat caatgtgttt ttttctgtat ttttgtaata
    41161 tctactgcaa taatcaccta agaataaatt tatatctctt gattttattg tattaaaatc
    41221 atcttctgta atatctttta cacaatctgc atttgttaat tctttggttt caatcaaata
    41281 tgaacaaaaa aaattaatat cttctaaata agcaagtcgt gtagaaactg caacagaacc
    41341 ttttaaatat ataaaataat ccttcataaa tacaggaagt ttattttcca aaagtagaca
    41401 tttttctatg agttcatttt ctttgataac aatattatca ggtttatctg atttattatg
    41461 tacttttata attttcttat cattattcaa aattaaacct cctaaaacta aaatataaat
    41521 cttttatatt attttttgat atttatacca agtgagtata aatatttaat ttaatcattt
    41581 tttacatttt taagagatta gcagtacttt agatgaaatt atcactaact attccctaaa
    41641 tccacattta aagactagtt gaatttaatt actctttttt tatttctcaa tatatttata
    41701 gaaatatttt aaaatatatt aatattttta tatgtttcta acgaaaccaa atattagtaa
    41761 aatatgtatt tatttaacta aagtttttaa atgggctgac taagcttaaa atataggata
    41821 ttatgagagt tactattgaa aaaataaacc ctattgccat aaagaaactt ataaatatgt
    41881 tatacttaac tattaaactg gatatattca agttatcaaa tccaaataag tatgtaattg
    41941 aacttattac aaaaataact ataactataa agctcagacc atttaagcta ttttttaaat
    42001 cagcactact aagtgacata tgtgtagata tagaatacat taaaaacata aatataaaat
    42061 acttcaatga atatacacta ggtgttaaca acattattct tataaaattg gaaatatcac
    42121 ctaaaataat gtgtataaaa ccatttatat ttaagttatt aatcatactt acatataact
    42181 ttaaatctat attttgagtc actttcatat aactatctgg cagtaatatc ttaaaaatta
    42241 aaataatcat tattgttcca cctataatag gtgcaattcc tataaagaaa tttcctatac
    42301 tttgataaag attatctttt ttatagctat gacttacata tcctagtata ccatcatttt
    42361 tactttctat tggtctgaaa aacttaactg aatttatttt atgcatgaaa attaaacaca
    42421 ttatcaaatg actcaattca tgaacaaatg ttcctatagc tccagttaca ataattccag
    42481 tttttccaaa agatgaaaat ataaatttat tatttagatt ttctgtaatg ctaaaaatca
    42541 atccaaatac aataaatatt cctatgaaac aaaaaatatt tattacactt atttttaaaa
    42601 tttcgattat ataaatcata agttaagtcc tcatgtaaaa gtatttaact actctacttc
    42661 atatccatta cagcttaaaa ttttacaagc attttctata tctttttctt ttactaagat
    42721 atagtctgta tcatacgtag aaattgcaaa tatgcttatt ttgttgtctg ctaacaaact
    42781 acttaactta gataaaattc ctacaagtga aaagtctaaa ggacctagta tcttaagtat
    42841 tctccactcc ttttctgact tgacatcaga aggaatatta ttatttaaac atacaacact
    42901 taattcatca tcagttctag ttatagagta aaacttctct gtatctatcc atgttggtat
    42961 tttactgtca ttatttaatc tacatactgc atactcttct accaataatt ttaatttcat
    43021 aatgtatatt tcctccaatc ttatttgttt acttaagatt atacattaaa agttacataa
    43081 aataaaacat ataaagtaaa aaaggttcac tatagtgtcc tatttctata gccaaccttt
    43141 aaaacttaat taaattctaa taaagagata tattatctag gttcaattat aagtttaatg
    43201 gctgttttct tatcaccttc gatttctata tcagtaaatg cgggaatgca aactaaatcc
    43261 attccactag gtgcaacaaa acctcttgct attgcaattg attttatcgc ttgatttaga
    43321 gctccagctc ctatagcttg tatttctgca acgcctcgtt ctcttagtac tccagccaag
    43381 gctccagcta cagaattagg attagatttt gatgaaactt ttagtacctc catttcaaca
    43441 tctccttgtt aatttgttaa agtatccatt acctaaagta ctaaatcata attgccattt
    43501 actaattaca atcatcattt aaagtatact tttataaaaa tatacttcta taatcgtttt
    43561 aagaaaacct ttattttttt taaccatttt attatgaaaa aactacaaat ttctttatat
    43621 tctataagca ttttccaata tttctaaatt ttttatatct ttgagctata atttcctctt
    43681 tatccatatc tttgtaatga tttaatcttt ctaaaatata agcttttatt tcttttgaaa
    43741 cagcttctac atctttttgt gctcctccaa gaggttcttt tataatctta tctattattt
    43801 ttaaatcata taaatcatta cttgtaagct tcataacatc acaagcttct ttagctttag
    43861 atgaatcttt aaatagaata ctagaaagcc cttctggaga aattacagaa taaactgaat
    43921 gttctaacat acatacatca tttcctactc cgatacctaa agctcctcca ctaccacctt
    43981 caccaattac aaaagtgatt actggaacag aaagtttact catttcaagt aagtttcttg
    44041 caatagcttc tccttgtcct ctttcttctg cttctagacc acaaaaagct cctgatgtat
    44101 caataaacgt tattataggt ctattaaatt tttcagcttg tttcataagt cttaatgctt
    44161 ttctgtatcc ttctggatga ggcattccaa aatttctttt tatattttct tttgtatctc
    44221 ttcctttttg gtgtccaact atagtaacat taaactttcc tatctgacca attcctccaa
    44281 ccatagaagg gtcatcttta aaaagcctat ctccatgaag ttctaaaaaa ttagaacaaa
    44341 tatgttctat gtattccaat gtcgtaggtc tctttatatc tctacttagt gttacctttt
    44401 catatgctga tagatgtgaa aaagcatctt ccttaagctt ttccaatttc tcattaagtg
    44461 aatttatttt atcacttaaa tctatattat ttgccttaga aatagttatt aattgtttaa
    44521 tatcattttc taagattttt attttttcac tattattttc aatcaaaaca gttcctcctt
    44581 tatgccctat ttcttgtgca tagcaagaat ttgatataat acttccttca tttttcttct
    44641 atctactatc atgtcaataa aacctttttc aagtaaaaac tcagaagttt gaaatccttc
    44701 aggaagtttt tgatttattg tttgctctat tactcttgga cctgcaaaac ctatcaacgt
    44761 atttggctca gaaattataa tatcaccaag catagcaaaa ctagcagtta ctcctccagt
    44821 agtagggtca gttagaactg atatatatgg caatgatttt tcttcaagct tagaaagtgc
    44881 ttgagaagtc tttgccatct gcataagaga taccatacct tcttgcattc tagctcctcc
    44941 tgaagcagaa caaataatta taggtaaatt attttcagca gcatactcta ttgaataagt
    45001 tattttttcc ccaacaatag aacccatact tcccatcata aaatcaggat tcattacaca
    45061 aataactgtt ttaattccat ttattcttcc ataacctgtg acaacagctt ctgactcttt
    45121 tgttttttcc ttataagagt ctactttttc tttgtattta ggaaaattta gtggattttg
    45181 aaattctaca tcagaattaa actccataaa agttccttta tcaatcaata aatttattct
    45241 ttttcttgct cttaagccat aatgtcttga acattttgga catacaaatg aattttcttc
    45301 tatgtccttt ctaaatacat gactatcaca acctttgcaa taggtccaaa atttatcatc
    45361 tacactattc tttttaaagt tgtcatctaa agaaatcgta acatacttag attcttttga
    45421 tgataaaaat ttttttatca taatctattt actccttata atgtagcaaa ttctttcttc
    45481 ataaagacat cttctataaa tttggtatga tgagtatctt ctctaaactc ctcacttgtt
    45541 acaagtgcct tttgaaattc tacatttgta ttaatacctt ctacaataat ttcgtctaga
    45601 gctctatata ttctacttat acattcttct ctagtttcag cccaacatat taacttacct
    45661 atcatagaat catataaagg tggtatagta tatcctgcat atacaaaagt atcaaatcta
    45721 acaccaaaac ctccaggtaa atttaatgat tctatcttac ctggtgatgg agcaaaattc
    45781 tttgatgaat cttctgcatt tattctacat tcaatagcat gacctctaaa agtaatatct
    45841 tcttgtttaa aaggaatttt atccccatta gctatagata tttgaagttt tataaggtca
    45901 agatttgtta ccatctctgt aactggatgt tcaacttgta ttcttgtatt catctctata
    45961 aaataaaagt ttttatcttt gtcaactata aactcaatag tacctgcacc gatataacct
    46021 actcctttta ctatatctac tgcaatttta tgaagttttt gtctttcttc atctgaaagg
    46081 tatggactta aagattcttc tattactttt tgatttcgcc tttgcataga acaatctcta
    46141 tcacctaaat gtattgcatt tgaatgttta tctccaaata cttgtacttc tatatgtcta
    46201 ggattttcta taaacttttc catatataaa tcactattag aaaatgctgc tagagcttct
    46261 gaacttgcca tatcaaaatt tgaaaaaagt tcttcttctt ttctaacgat tctcatacct
    46321 tttccaccgc ctccacttga ggcttttatc ataacaggat atccaatttc tcttgcaact
    46381 tctaaagctt cttccttagt tttcaaaaca gattctgaac caggaacaac agggatattc
    46441 aattccatca tagtttctct tgctctagat ttatctccca tcaaaatcat ttgttctctt
    46501 gttggaccta taaaaataat attattatct tcacattgct ttgcaaattc aggattctct
    46561 gataaaaatc caaagcctgg atgaatagca tcacacttta atttaagagc aagatagatt
    46621 atattttcta tattattata actcttactt atattatttg gtcctataca aatagattca
    46681 tcagctatat atctatgaaa acaatcttta tctatttcag aatagatagc aacagttttt
    46741 atacctagtt ctttacatgt tctgatgatt ctaactgcta tatcgcctct atttgcaact
    46801 agtatttttt ttatcaactt aaatacctcc tactacaatg gttttatctt aaataaaggt
    46861 tgattatatt ctactagttc ttcattacta actaatactt caattatttc acctttaatt
    46921 tcacttgtta tctcattcat aagcttcatt gcttctaata tacataaagt gtcgccttct
    46981 tcaactacat cgccaacttt aacaaagcta tctgcatctg gacttggaga atcatagaat
    47041 gttcccatca aaggagctac tactactgat agattctcat tagatgtgac aggtgtatcg
    47101 tttacaactg gcttttctaa agaaagatcc actactgatt cttgttgtac acttaaaaca
    47161 ggtgaacttg tagattgagc ttttttagat acttctaatc ttaaatcgct actttcaagt
    47221 tttacatatt ctaagttagt tgagtctatg gcctttaata attccttaat ttcattgatg
    47281 ttcattttgt tacccccttg tattattacc tggtgctaat attatatcac aaaaaacaaa
    47341 tttataaaag aaaaagtgct gactagagaa tcaacacatc aatatatctt ttaattttaa
    47401 attaacaacc agtaacttat atttgtttta attttccatt attaatataa attataagta
    47461 taaaatgaat aaaattcaat attttttgca aaatacaaga gaatacaaat taatacctcg
    47521 ggaattttta agacaaatac caatattatg caatttagac ttcgctttct ttcaaattag
    47581 tatcattttc tttattttta cataggatgt actttaattt agctatcaaa acattgctat
    47641 ataggttaaa agttattaga attgcaggaa ttataaagta aactctagca cctataaaac
    47701 caaaaactac tgtaataagt attaatcttt tatttaaagt aaaaaaaggt atattaaaaa
    47761 tacttttgtc ggatgtttct ggagtataag aatatataac tccaaaaatc aaagtaagca
    47821 tacaaagtgg aagtagtgta taacttgaaa ttattcccaa aaatcctata ctttcaaaat
    47881 atttgctagc actatttgta taaacaaaca tgtatccatt taaatatgcg catatacatg
    47941 atataatgcc aaaagttccc cctaaggatt gagtgtacca attaaaaaat ttagattcca
    48001 tatacctcac ctcctaaaac tgtatatata atgatatgca tttaaatagt aaaatatatc
    48061 tttatatgtt tctgaataaa attttgttaa tttcattaat ttatgtttaa gaaatttgaa
    48121 gtatagtgta gtatatatta atgtagatga agttttatag tacttatttc atagtgctta
    48181 atcaagttat cttattatgg attcaaaagt tgtttgtagg gttttatatt ttgttgagct
    48241 gaaaaattta ttataaatga agggatgttt tcgctttggt agatacatta gaatttggtc
    48301 ttaaaatttt attttttata ttatcaataa tttggatggg taaaataatg attttaagaa
    48361 ctgataagca aatagtaatc aatcctttgc ttattggtat atctgcagtc cttgtgatgt
    48421 tgcatacctc acagagtaac attgaatttt ttggattaga tgtccaatat ataagaatag
    48481 ttttgtatat aatatattca ctgataattt taataggaat atgggctact aacagaagaa
    48541 atggaatttt ttagataatc tatttctata aaatttaaag gttgctagta acaaatcact
    48601 aatattagcg ttctgttact aacaaccttt attttgatta tacacttaaa ttgaaaatag
    48661 aatgattgaa taagttgaca gattatttta cattgacagt aattagaact tcttgagatg
    48721 tatttaaatc atttttgtcg cttagtataa cttttaccac tgcttgacct gttttattac
    48781 ctttaattat catatcttca catgaaagta cactcttatc acttacagat acaataaaat
    48841 ttaaatcttt tggaaggtta tcagtaactt cccagtattt ataataacta tcaattttct
    48901 tcgtaattat ttttctcaaa tcaatacttt caccttttgc tatatcaaca ttatgatatt
    48961 tagagtccag tttatactga ttatcttttg tatcaataaa atttaaatcg caatttacat
    49021 ccacaccatt atgagaaaga gaatttacta catcgtcatc aatcaaataa ttattggcaa
    49081 gatttattga atccatatct tttagactta aagcttcctt aggaacctct tttatattat
    49141 tattccaaaa aataaaattg tctacatttt caagatatga tatacctttt aaatttgaaa
    49201 tactctcacc agttgcataa aattctccat ctaaagcctt tatattgctt atagtcatct
    49261 catctggatt tatatatctt tttacaactg ctcttaaatt tgcatcagga aaaatatctt
    49321 taagttgatt ttcgctcaaa accttatcat ttgaatttat tggacttaaa gctgctgcag
    49381 aaataataaa tggtattaaa ttagctaaat tcatagtacc ctcccacata aattttttat
    49441 tataataatt taatttctat gagagggttc tgtttccttg ttatatatac ctactttcgt
    49501 acactttatt aatataaatt aaaataaatt tctactatta aacttataaa catattacta
    49561 gttacgtttt ttagcttcac atctaaatat tcgcatacct tgtgacataa atttgtcttc
    49621 atactctgtt gtaacattat tttcatactc actatttcct aaatcaagag aaatattctt
    49681 taatagccaa ttatttgcag caatttcatt taaactaaac tcgaataatt tttcattatc
    49741 tgttttaaag tgaatttcgc catcatcttc caaaacccta ttgtacattt ctaaaaatcc
    49801 agaatgagtg agtcttcttt tactccatct atttttaggc catgggtcac agaaatttat
    49861 ataaattctc gataattctt tagcttcaaa gtaatccaaa atattgctta catctcccca
    49921 taaaaatagt atattattca aattagaagc atctgctttt tcaacagcct ttaaaagaac
    49981 ttcttcctta agttccatgg caatataatt tatgtcaggg ttctgcttag ctaaagttgt
    50041 tataaactta ccttttccag tcccaaattc aacatgtatt ggattatcat ttctaaactt
    50101 taaattccac ttgcctttta atttgtctat atcgtctctt aaaacatatt ttgtataact
    50161 taaaagcttt tcatctgcac ctttttttcg tcttcttctc acaataattc tccttacata
    50221 taaaaaatta gtaaaataca tttttattat acattatctc accaaaaaat aaaaagaact
    50281 ctcttaaaat agaaatttat ttctataaag agagttctaa acttactaaa aacttaatct
    50341 tagtggcacc ctgaacaacc agagcatcct cctccacctt gatctttcat attttcagga
    50401 ataactctaa atccaaatcc tgtatcttct attattatat ctccatactg actatattca
    50461 tcagagctca ttacaaattg taattctcca acttcataag ttaagtcatc ttcttttttc
    50521 tcatctaaag ctaatccaaa agaaggacct ccacaaccaa cacctgcaaa gtatactctt
    50581 atattattag gtttatcttg attgtctttt aagatacttt ttaaagtatc aatagctgtt
    50641 tctggtaaag taattttcat caaatcattc cctttcaaat attttattaa aacaatatct
    50701 attatatacc catacaaggt acataatcaa ttagtttttg catattttac taaaacttct
    50761 acctaatatt acctaaggct ttaaaatatt ttatataata ttt
//
LOCUS       DAEOSP010000004        48057 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.004, whole genome shotgun sequence.
ACCESSION   DAEOSP010000004 DAEOSP010000000
VERSION     DAEOSP010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48057
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.004"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(174..1535)
                     /gene="rlmD"
                     /locus_tag="KRH54_000164"
     CDS             complement(174..1535)
                     /gene="rlmD"
                     /locus_tag="KRH54_000164"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG8577848.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVLL
                     RRK"
     gene            complement(1562..2284)
                     /locus_tag="KRH54_000165"
     CDS             complement(1562..2284)
                     /locus_tag="KRH54_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG8577849.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            complement(2284..2613)
                     /locus_tag="KRH54_000166"
     CDS             complement(2284..2613)
                     /locus_tag="KRH54_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBG8577850.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESIDLAFTVFEKEKY
                     YENGEHF"
     gene            2943..3869
                     /gene="fba"
                     /locus_tag="KRH54_000167"
     CDS             2943..3869
                     /gene="fba"
                     /locus_tag="KRH54_000167"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBG8577851.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(4004..5635)
                     /locus_tag="KRH54_000168"
     CDS             complement(4004..5635)
                     /locus_tag="KRH54_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8577852.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(6118..7155)
                     /locus_tag="KRH54_000169"
     CDS             complement(6118..7155)
                     /locus_tag="KRH54_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG8577853.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(7174..7959)
                     /locus_tag="KRH54_000170"
     CDS             complement(7174..7959)
                     /locus_tag="KRH54_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG8577854.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(7992..9125)
                     /locus_tag="KRH54_000171"
     CDS             complement(7992..9125)
                     /locus_tag="KRH54_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG8577855.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(9173..10300)
                     /gene="hadC"
                     /locus_tag="KRH54_000172"
     CDS             complement(9173..10300)
                     /gene="hadC"
                     /locus_tag="KRH54_000172"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBG8577856.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(10300..11526)
                     /gene="hadB"
                     /locus_tag="KRH54_000173"
     CDS             complement(10300..11526)
                     /gene="hadB"
                     /locus_tag="KRH54_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBG8577857.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(11541..12341)
                     /gene="hadI"
                     /locus_tag="KRH54_000174"
     CDS             complement(11541..12341)
                     /gene="hadI"
                     /locus_tag="KRH54_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBG8577858.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(12380..13579)
                     /gene="hadA"
                     /locus_tag="KRH54_000175"
     CDS             complement(12380..13579)
                     /gene="hadA"
                     /locus_tag="KRH54_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBG8577859.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            14256..15254
                     /locus_tag="KRH54_000176"
     CDS             14256..15254
                     /locus_tag="KRH54_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBG8577860.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            15616..16143
                     /locus_tag="KRH54_000177"
     CDS             15616..16143
                     /locus_tag="KRH54_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBG8577861.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            16170..16766
                     /locus_tag="KRH54_000178"
     CDS             16170..16766
                     /locus_tag="KRH54_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBG8577862.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEIAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(16831..18414)
                     /gene="asnB"
                     /locus_tag="KRH54_000179"
     CDS             complement(16831..18414)
                     /gene="asnB"
                     /locus_tag="KRH54_000179"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG8577863.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDRYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(18980..19816)
                     /locus_tag="KRH54_000180"
     CDS             complement(18980..19816)
                     /locus_tag="KRH54_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG8577864.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
     gene            complement(19840..21306)
                     /locus_tag="KRH54_000181"
     CDS             complement(19840..21306)
                     /locus_tag="KRH54_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG8577865.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASAIATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            complement(21309..23171)
                     /locus_tag="KRH54_000182"
     CDS             complement(21309..23171)
                     /locus_tag="KRH54_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG8577866.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            complement(23418..24281)
                     /locus_tag="KRH54_000183"
     CDS             complement(23418..24281)
                     /locus_tag="KRH54_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG8577867.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            complement(24336..25268)
                     /locus_tag="KRH54_000184"
     CDS             complement(24336..25268)
                     /locus_tag="KRH54_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBG8577868.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            complement(25354..25905)
                     /locus_tag="KRH54_000185"
     CDS             complement(25354..25905)
                     /locus_tag="KRH54_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8577869.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            complement(26134..26625)
                     /locus_tag="KRH54_000186"
     CDS             complement(26134..26625)
                     /locus_tag="KRH54_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577870.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            complement(26653..27213)
                     /locus_tag="KRH54_000187"
     CDS             complement(26653..27213)
                     /locus_tag="KRH54_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG8577871.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            complement(27365..28522)
                     /locus_tag="KRH54_000188"
     CDS             complement(27365..28522)
                     /locus_tag="KRH54_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG8577872.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            complement(28559..29500)
                     /locus_tag="KRH54_000189"
     CDS             complement(28559..29500)
                     /locus_tag="KRH54_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577873.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            complement(29587..30279)
                     /locus_tag="KRH54_000190"
     CDS             complement(29587..30279)
                     /locus_tag="KRH54_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG8577874.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            complement(30388..31200)
                     /locus_tag="KRH54_000191"
     CDS             complement(30388..31200)
                     /locus_tag="KRH54_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBG8577875.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            complement(31251..32162)
                     /locus_tag="KRH54_000192"
     CDS             complement(31251..32162)
                     /locus_tag="KRH54_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8577876.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            complement(32221..32952)
                     /locus_tag="KRH54_000193"
     CDS             complement(32221..32952)
                     /locus_tag="KRH54_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBG8577877.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            33041..33292
                     /locus_tag="KRH54_000194"
     CDS             33041..33292
                     /locus_tag="KRH54_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577878.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            33526..33945
                     /locus_tag="KRH54_000195"
     CDS             33526..33945
                     /locus_tag="KRH54_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577879.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            33970..39027
                     /locus_tag="KRH54_000196"
     CDS             33970..39027
                     /locus_tag="KRH54_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBG8577880.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSNLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(39212..39949)
                     /locus_tag="KRH54_000197"
     CDS             complement(39212..39949)
                     /locus_tag="KRH54_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8577881.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(39968..40903)
                     /locus_tag="KRH54_000198"
     CDS             complement(39968..40903)
                     /locus_tag="KRH54_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG8577882.1"
                     /translation="MDNNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            41111..43687
                     /locus_tag="KRH54_000199"
     CDS             41111..43687
                     /locus_tag="KRH54_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG8577883.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YTNKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            43699..44370
                     /locus_tag="KRH54_000200"
     CDS             43699..44370
                     /locus_tag="KRH54_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8577884.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            44397..44738
                     /locus_tag="KRH54_000201"
     CDS             44397..44738
                     /locus_tag="KRH54_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577885.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENEDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(45098..47740)
                     /gene="adhE"
                     /locus_tag="KRH54_000202"
                     /gene_synonym="adhC"
     CDS             complement(45098..47740)
                     /gene="adhE"
                     /locus_tag="KRH54_000202"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG8577886.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT        16112 a         8109 c         5797 g        18039 t
ORIGIN      
        1 atttaatatc tatttctata attaaaactt tttttatttc gtattcccac actccatata
       61 tataactaca gacacatcaa aacacctttt caattcttta ttcctaattt tattaccctc
      121 taatctaaat attatatgtc tatgaaactg tctaaagctc aagatttcta gcattatttt
      181 cgacgtagca acactacagt ctcacaatgt ggcgtatttg ggaacatatc catacactta
      241 accatcttaa tctcataacc atacccctta aaatcaacca aatcaacaac caaagactta
      301 ggattacaag aaatataaac aatctcctta gcaccaaatc cacaaatatc cctaatagca
      361 tccttatgaa tgcctggtct aggagggtca acaataatca aatcaggctt gtcctttaaa
      421 tccttaacaa ccttagccac atccccagca ataaactcac aattagtaag tccattcaac
      481 ttagcattct cattagcagc aacaacagct tcctcaacta tctcaatacc atacactttc
      541 ttagcagcac cagccataac ctgtccaata gacccagtac cactatacaa gtcaaataca
      601 actttatcat tgtaatctcc aataaaatcc ctagcaatac tataaagaac ctctgctccc
      661 ttagtattgg tttggaaaaa cgaaaatggt gaaatcttaa acttaagacc taaaatttct
      721 tcttgtatat aatctcttcc atacaatact cttaactcat cacattggac tgcatctgct
      781 aaaccatcat taatagtatg aagtacacct actaactcag cttttaaatc aatccctaaa
      841 agcatatctt taaactcata catatcaaaa tcttcttgag aagaagttac tatattaacc
      901 ataatctcat tagtatgaat cccttttcta actacaagat gtctcaaata tcctttgtga
      961 ttcattgttc tataataagg aagctttttt tcattgaaaa actctacact agaaactaat
     1021 atctttgaga agtcttcatc aaccaacata cactcctcaa cagtttgtat atctatatgc
     1081 tttccttttt tatgaagacc taatgtcaaa ggtccatttt ttacttcatc tccaaaagta
     1141 tattccatct tatttctata atatttgttt tctggacttc cttctatccc taaaaactga
     1201 aatccaaata aatcttgctt taaaaataaa tccataactt gtttttcttt tatttctaat
     1261 tgtttttcat atggcacaga caatagtgta catcctccac actctctaaa gtgcttgcaa
     1321 gtttcttcag tttcaagtgg tgagttctct aataattcta tcatcttaac ttcagctttc
     1381 ttacttctaa cttttttaac tccagctctc accttttgac cacttattcc acccttcata
     1441 tggataactc tatctccaac ttggcttaaa gatgtaccac caaattccat tttatctact
     1501 tcaaattcta taacatcttt tttcttcaca gacattcctc caatttacta tagtatcttt
     1561 attatctttc ttcaatcaat tttacttctt catctgttaa ctctctatac tcacctaact
     1621 ctaaactttc atccaatttt aaattgccca tagacaatct ttttaagtat actacttttt
     1681 tgcctacgct ttcaaacatt ctttttactt ggtgaaactt accttcatgt attgtaagct
     1741 ctatttctga ttcatcatca ctttttaata tatttaactg tgatggcata gttttatatc
     1801 cgtcatcaag cactacacct tctaaaaaag cttctacatc ttcttcagta actactccat
     1861 caatttttgc ataataagtc ttaggcacat gcttcttagg agataaaact ctatgagaaa
     1921 gctttccatc attagttaaa actaataatc cctctgtgtc tttatctaac cttccaacag
     1981 gaaatggttc aaaagctaag tatgataaat caattaaatc taatactgtt gggtcatatt
     2041 tatctgtagt tgctgatata tatccatcag gtttattcat catcaaatat atatattctc
     2101 tatatataac ttcttctcca ttaaataata tctcatcatt ttcagtgtct acttgagttc
     2161 ctggatttga aacttcactt ccatttacta ctacagaccc ttgtttacaa tacttcttta
     2221 tttcacttct acttccataa cctaaatttg aaagaatttt atctatacgt tgctttttag
     2281 ccattaaaaa tgctctccat tctcataata tttttctttt tcaaatactg taaatgctaa
     2341 atctatactt tctacatcta aattatttct tatactatca gttattattc ttgctactat
     2401 atcttgtact tcttgtcctc tatcaaacca agctacttca acaaaaggat atacatctgc
     2461 tattttacca tctcttattg caactgactt tatacattct atttcaaagt agtctcttgg
     2521 acattttaca gcttcaacta aatcatttat cattttttca cttattttac atatatcatt
     2581 ttcattaatt cctcttattt ttatctgtgg catagtttat ctcctttatt ttaaattttt
     2641 agtaaattat catccactta attcctttcg ataacgtttg aagtggtaac ttcttggtca
     2701 aggcaactat tgttatcagt atctccaaac taataaggta gttcctaccc ttatagttag
     2761 cattatatag ctagttttcc taaattgttt atattcttta ttattttatt taatcctatt
     2821 gtcttctatt taacattata cttgtaattt atagttatta caatttacta tatacttttt
     2881 ttgatttaac gttataatat aagtgatggg taaattttaa aagttaatag gaggagttta
     2941 aaatggcatt aattactaca aaagaaatgt ttaaaaaagc ttacgaaggt ggatttgcta
     3001 taggtgcatt taacataagt gacttagaac aattacaagg tgttttaaaa gctgctaaag
     3061 ctaaaaattc ttatgttatg atacaagctt ctatgtcagc tgttaaatat gctggacctc
     3121 atacattagt tgaaatggtt aaggctgctt ctgatgaaat aggtgtagat gtagctcttc
     3181 acttagacca tggtccaaat atggatgcta ttaaaacttg tattgatgct ggtttctctt
     3241 cagttatgat agatggttct cattttgact ttgaagaaaa tgttagaata actaaagaag
     3301 ctgttgaata tgcacactct aaaggtgttg ttgttgaagc agaacttggt gttctagctg
     3361 gaacagaaga tgatgttact tctgatgttc acaagtatac tcaaccagct gaggcagttg
     3421 agttcgttga aagaacaggt gttgattcat tagcaatagc tattggaact tctcatggtg
     3481 ctttcaaatt caaaggagaa gctaaattaa gattcgatat actagaagaa atccaaagca
     3541 aattaccagg tttcccaata gttcttcatg gtgcatctgc tgttgaccaa aacgctgttg
     3601 ctacatgtaa tgaatttggt ggaaatatag ctggtgctaa aggtgtacct gtagatatgt
     3661 taagaaaagc atcttctatg gctgtttgta aaataaacat ggatactgac ttaagactag
     3721 ctatgactgc tgctatcaga aaattcttag ctgaaaatcc aaaagaattt gacccaagaa
     3781 aatacattgg tgctggtaga gatgctatac aagctgtagt tgaaagcaag atagatgatg
     3841 ttttaggttc tgctaactct ataaactaat ataactttta aatatttata aaagaaaaga
     3901 gatgacttaa agtaattaaa tttattttta agccatctct tttaagttgt ataaattatg
     3961 tatcttataa tattttaatt atattgtctt tttaaactga cagctaaacg tgaatatgtc
     4021 cactctttat ctttctatgg atggtagaag gatatatacc tataacttca gcagcctttg
     4081 ttattgaacc atacttattt atagccttac ttatcattat ttggtccact attttatatg
     4141 catcattaag attcataatt ccatttacca ctattggtga taaatcatct gtttcattat
     4201 caatcatatc taaatttatc agcatgttaa attcatcttc tccaattaca tttttagctg
     4261 ataatactac aaatctttca atgatgtttt ttaattctct tatgtttcct ggccaagaat
     4321 attcttctaa taactcaata actttaggcg aaacttctac ttctctattg tacttttcat
     4381 tgtaaagttt taagaaataa tttactaatg gtactatatc ctcttttctt tctctaagag
     4441 gaggtatttt tactggaata acacttaatc tatagtacaa atcttgtcta aatttaaggt
     4501 tatttctaag gtcttcatga gaaatattag tagcacttat atatctaaca tctattttta
     4561 taggcttact accacctatt ctagtaatag tattttcttg tataacccta agtaattttt
     4621 tttgcatctg cattggtaac tcacctatct catccaagaa aatagttcca ccattcgcct
     4681 cttcaaaaag tcccttttta ccttttttct tagctccagt aaaagaacct tcttcatatc
     4741 caaaaaactc tgactctaat aattcatttg gtatagctcc acagtttata gctataaaag
     4801 gtttgtctct ccttgaacta tacttatata ttgttcttgc tattatttct tttcctacac
     4861 ctgattctcc atttataaat atagaagaat ctgaggctgc agctctctta acaacttttt
     4921 ttatattgtc cataatctta ctttgactta tatactctgt gtcttctgat atatcactat
     4981 attttatctg agattcaagc ttaataaccc ttgaaatatc tctccctgtt agtactcctc
     5041 ctgtaagctt tcccttagaa tcccatcttg gaactgccgt atatgcaata agcttcccat
     5101 ctgggtaaac gatatttcta taaatctttt tcttcacttt aagcatgttt aatattattg
     5161 gttcttcaac taatccttgt ctcacaaggt ctgttatatt tctaccaagt acctcagttc
     5221 ttttaaaagg taatatctca tcactgacag tgttcatctt ttcaattacc ccatctttgt
     5281 taaacacaac aacaacttca tctatatgct ctattatatc ttcaattact ttatcagata
     5341 ttagagactt ctcaaataaa taaactatat tattttcatt attattatct ttcatattgt
     5401 atatatgtgc agtcatcctt aactctctga aagctatttt ctttttacta tctgtcagta
     5461 tttctgtttt gtcaaaattt gaatctaatt catttatatt tctaggcttt tttatatcta
     5521 gaaaactaaa aatactatct attagatgat tacagtatat tatttcgcct gattctttac
     5581 ataaaatggc tggattttct acatgttcta taagccctat taaattatta tccattaata
     5641 ttcgcacctt tcattaagta atttattgaa ctacatacaa aatttattgt ttgcttacaa
     5701 taaataccat cagcacatat aatcaactcc ttataatatt cgtcttttca agtagtttat
     5761 ttcattaaaa cgtaactaag atataatttt aatgattata gtttgttaaa aatttcattt
     5821 tgcttttaat tgtattttat cacatattta gtaattattt gtctatttaa tacaatttaa
     5881 ttaaatttaa gatttattta aaaaaacaaa gataatcctt acagaatata actcacaatt
     5941 tgtaagcaat atacatataa ggattatcca gattatcttg tattataaaa ttttattcaa
     6001 acagactttt taataactga caaatacaca ctagcaataa cttatcaaag ggttaataat
     6061 tatgttacta actaataaga cttattcaat cagtaatatt tatatagttt gtatctatta
     6121 agcttttata gcttctaata aatcatcaac gctatctaat ttttctataa gcattggaac
     6181 tatttcatgt aagtctccaa ctactccata gtcagcgatt tctaaaattg gagctgctgg
     6241 gtttttgttt atagcaatta tgaaatctga tgattgcata cctgctaaat gttgtattgc
     6301 tcctgatata ccacaagcta tatataggtt tggtttaaca gtagttcctg tttgacctac
     6361 ttggtgagaa tggtctatcc atccagcatc aacagccgca cgagaagaac caactactcc
     6421 acctaatttg tcagctaatt tcttaagcat ttcaaatcct tctggtccac ctaatcctag
     6481 accacctgat actataacat ttgcatctgt aagagatact aaatctttct tagttttaac
     6541 tgtttctaaa acagtagttc ttatatcatc ttgagttatt ttgtagtcta atgctataac
     6601 ttcaccagtt cttgtttcgt ctttttcagc tttatccata acaccaggtc taactgtaga
     6661 catttgaggt ctatggtttg gacaaatgat tgtagccatt atgtttccac caaatgctgg
     6721 acgagtttgt tttattttct tatcttctgg gtctatttct aacttagtac agtcagctgt
     6781 taatccagtt ccaactcttg aagctattct aggtgctaag tctctaccta tatgagttgc
     6841 tccgaaaagc attatttctg gttttatttc atctattgca tcttttatta cttttgtata
     6901 tgcatcagtt gtatattttt ctaaaagagc atcatctgca acataaactt tgtcagctcc
     6961 aaaagtgatt aattctttag ctaatccatc aacatctttt cctaataata ttgcacaaag
     7021 ttctgcatct acttctttag ctaattttct tccttctcct aataattcta taactactgg
     7081 agctattttt ccttctcttt gttctgcaaa tatccatacg tttttataag aacttaaatc
     7141 ttttatatca ttcatagttt gtactcctcc aacttatata atgtgctttt gttttagacc
     7201 gattattatt ttactagcta tttcatcagc tttaccttct aagatttctc cttttcctct
     7261 tggttcagga gtaaatgatc taaatacttt agttggtgat gcttttaaac caacttcttc
     7321 tttatttaca tctaaatcat caattgtcca aacttttaca tcatgtttat atgcttttac
     7381 tattttatca acagacatgt atctaggtgt atttaactct tttactgctg ttaaaagtac
     7441 aggtgtactt acttttatta attcgtatcc atcttctaat tgtctttgaa ctattatatc
     7501 ttttccttct attttgaaat cttgtacata tgtaacttga ggaatgtcta atttctctgc
     7561 aatttgtggt ccaacttgtg cagtatctcc atctattgct tgtcttcctg caaatattat
     7621 gtcatagtca cctactttag atattcccgc tgcaatagta tttgatgtag cccaagtatc
     7681 agctcctcca aatgctctat cacttaataa tattgcatca tcagcaccca tagctaaaca
     7741 ctctcttagc ataaagtctg cttgaggagg tcccattgtt ataactgtta tagttacatc
     7801 atcatgctca tctttaattt ttaaagcttc ctctaatgca tttgcatcat ctgggtttaa
     7861 tatacttgga actccatctc ttataagagt acctgtttct ttatttattc ttacttcgtt
     7921 agtatctgga acttgtttta cgcaaactaa tattttcaaa gtctatacct ccaatttcat
     7981 tagattattt attattttaa tacgtttgat gaaattacca ttttttgaac ttctgatgtt
     8041 ccttcgtata ttgatacgat tctagcatct ctgtacattc tttcgatttc ataatctttt
     8101 ataaatccat atccaccgtg aagttgtaat gctttataag caacttcatt tgcaatttct
     8161 gatgcatagt atttagccat agcagattct tgagcaactc tcattcctgc atctctcttt
     8221 tgtgctgcat cataaactaa tcctctagct gcacaaactt ttgtttccat gtcagctata
     8281 gtaaattgag tattttggaa tttagctata ggcttaccaa attgaactct ttgttttgta
     8341 tatttaacag cttcatctaa agcaccttga gctattccta gagctaaggc agcaacacct
     8401 attctaccaa cttctagagt acccattgct atcttaaatc cttgaccttc ttttccaagt
     8461 aagttttctt ttggaacttt aacgttttca aatattaagt cagaagtttc agttcctctt
     8521 atacccatct tatcttcatg agctcctgtt gagaaaccat cccatttact ttctactata
     8581 aacgctgatg ttcctctaag tcctttactt ctatctgtaa ctgctattac tatagctaca
     8641 tcacagaatg gtccattagt tatgaaagtt tttctaccat ttaatatata gtggtctcca
     8701 actaattctg ctgttgtttg ttgtccacct gcatcagaac cagctcctgg ctcagttaaa
     8761 ccaaatgctc ctattaattc acctgttgct atacctttta agtatttttg tttttgctct
     8821 tcagtaccat tgtaaagtat tggtaatgaa gcaagagaag aaccagctgt aacaaaagta
     8881 gctgttgaag cacatcttct agctatttct tccattacta tagcgtaaga aacatatcct
     8941 gctcccccac caccatattc ttctgggata ttactgctta taaattttgc atcagctaat
     9001 tttttgatta attccttagg cattacacct gttttatcca tttctgctgg taaagtatct
     9061 aattctttac taacaaaatc tcttactgct tttcttaaaa gttcttgttc tttgttatat
     9121 aacatagctt cctccaattt tcatatttta tagtaaattt attttaatga acttacgcta
     9181 aactcatcat ctcagcaaaa gtttgaatac gagttctggc ttgttcatta ttctgagttt
     9241 gttggtcgat ttcaacatat aaagtaggta ttccactatc ttctatatct tttctaacta
     9301 aaggataatc gtattcttct gggtcacaga atttcatcat acagaagata acaccatcta
     9361 tatctttctt tttaacttca tctactataa gtgacccacg ttttttctta gggtcataag
     9421 ctaatgaaca tccttctatg tttgaccatt gtcttgctaa tctttctaac gcatcatcac
     9481 ctgctggtac atctgttctg aattgtcttg tttcttgtgc taagtcgtca gctacaactg
     9541 atatattgtt gtcttctaaa atgtctaata tatctttaga atcagctaat atacctgtta
     9601 ataaaacttt ctttccagaa cagacttctt ctggcatagc atttaatttt gctattaaat
     9661 ctttcactag ctcagtatgt tcttcttttc tcataaagaa cccactctta attactaagc
     9721 ttcttattga tggttttata gtattagaat gtttataagc tacttcaaca aagtctctca
     9781 tagtttttct atgttcattg taaacttcta tactttcatg aatttttgct tcttctattt
     9841 catatccaca aatttcttca agttctcttt ttacgccttt gtactcactg attaagtatt
     9901 ttacaccagc ttcaagtttt ctattttgtg ggtgtactaa tgatatatat tttatatgag
     9961 gtactgctga tttccagttt tgtcctaaac aaattagtgt atcacacata cctggtataa
    10021 taactccaga taattcatca taagcacctt ttaatccata ttctaaacat gattgcatta
    10081 ttgaacatgc aaatgcaggg aaatattgtt tagctaaatc taattctgtt tgccctcccc
    10141 atataccaac tggaagcatt ccagctgcat gtataatttc ttctgggcaa taaactggga
    10201 aacaacctat agctttttta ccagtttcac ttttatattt ttttacagcc gcatttggat
    10261 tttcaactac ttctttcatt ttagataaaa tagcttccat tatatctcac ctctattaag
    10321 tttttttctt tcttccatta cttcaactaa accttgaatt cttgtttcaa attgagcatt
    10381 agtgaaagct ctagggtctg cttggtcacc atcaaatcca gcatatggta atccagtttc
    10441 ttcagctgct cttctttgca tttcatattg tattaaactc ataagtttac agcttctgtt
    10501 catatgatag aatgctccat cacatttacc ctctactaaa gaatcaactc tatattttgt
    10561 catacggtct aggtttacat tgttaaacat agtactatat gctactgcca ttccatctaa
    10621 atcattaact tcatattgta atgcccaagc atgtgggtaa acactacctg tcatgttaac
    10681 tccaaattta gctaatgttt tcatcttgta tcctatatat ggccaacaag gtataccttc
    10741 catcattatt ctgtattttt cttcccctct gaaagaagat ttaccagttt tcatgttgtc
    10801 ctctaattct tctataagta acttaaatgc ttctgtagtt tcttttttac ctctagcaca
    10861 aactattaca gccatgtaag taaataagtc aaatccattc attggagaag gagaagaatc
    10921 tgctggtaaa ctcatagaat acttccatag tcttccgttt tcagctgata ttttcattac
    10981 ttcttcaaac ttcttagggt caaatttctt tcctgatata atttctagtt gttttatagc
    11041 ttcttcaaat tgagctttaa tataatctat tcttgattga gtaacttcgt cttcattatt
    11101 gaaagttgta tcaatcatta ttaaaggtat atctaattct cttgaaatat tttcatacca
    11161 ttttataact tggttacata tattgttaca gcaaagtagg aaatctggag ctggcatatc
    11221 caaagcttca catccaccat tttctaaaag accaaaattt gttcttgcat atgcacatag
    11281 gtcaatagaa tatcctttag attcagctat ttcacataat tctaaagaac cttttttagc
    11341 tgctactcca gctgcttggt tttctggata taatacgttt aagtcaaata cttctgctaa
    11401 ctcttgagga aatactgatg ttgaccaacc tacaggtctt ccttcttctt tagctttaaa
    11461 tgcatttgca tattgttcag ctaataaatc attaattact actctagctt cttttttttc
    11521 agacattttc atccctcttt ttatatattt ttcacttctt tttgtgattc ttttgcttca
    11581 tcaaaagcat ataacgctgc tcctaaagca ccagttaatt gaggtatatc aggtacaata
    11641 atttctgtgt tgatttctct tgccatagct cttacaatac cactatttct agcaactcca
    11701 ccaaccataa ctacatttct ttgtactcct attcttttta ctaggctaga aactctcttt
    11761 gctactgaag tatgaatacc tgccactata tcttcaattt ttgcattttc agataaatgt
    11821 gatataacct cagactctgc aaatactgta catgtactgc ttattgatac ttcattttga
    11881 gaattcatag atatacttcc gagttcagat acatcaacct ctattatttt tgccattaca
    11941 tctaaaaatc ttcctgtacc tgcagcacat ttgtcattca taagaaagtt taatagtctt
    12001 ccattattat ctaattttaa tacctttgca tcttgaccac ctatgtcaat aatggttctt
    12061 gtctctggaa ttatgaaatt tacccctcta gcatgacagc ttaattcact tatttgctta
    12121 tcagcatctg aatagttcat tcttccatat cctgtaacta caactttttt aatatcttct
    12181 cttgcaagac ctgtcttgcc atataatttt tctaaaactc ttgatggtcc agtagtccca
    12241 gtaccagagg atattgtttc agaagctaca atatcttccc cattctttaa gattactccc
    12301 tttgatgcag ttgaacctat atctaatccc attgtgtaca tctttagcat tctcctttta
    12361 aaaaatctag aattactaat taatatctta caactttact atctttaaag ttatttattt
    12421 tctcttcatc gtagcctaaa gattttaata cttctacagt atgttgacct acttttggtg
    12481 ctggtgtata ttctttaatt ccttcatttc taaacataac tggagtatta actaagacac
    12541 ctgtatttcc gctatcgtat gttttcttaa ataagaagtc atttgcccaa gcttgttcgt
    12601 catctaataa atcttcacag ctttgaattt tttcaaatgg taagtctgct tcttctaata
    12661 aagctgacca ctcgtctaat gttttttcta acatagcttc tccaactatc ttaactaaat
    12721 cttcaacatg attaaccatt gaatctatgt tattatatct atcatcttct aatatatatt
    12781 ctctatttat aaccttacag aatttgccta accacttgtt gtattgtatt aaagctaatt
    12841 gaatccatct tccatcttta catttatatg tagtcattaa tgggctgttt ggattttctc
    12901 ttgataaagg catttcattt ccgtattgtg ctgttgttat cattgttccc attccatata
    12961 tagctgtatg gaaaagactt actgttactc tctcaccttt accagtttga gcttttttat
    13021 gtaatgctgc taaacttcct gctgctagtg ctagacctgc atagtggtca ccaaatcctg
    13081 ctgctgtatt tgctggagat gttccttttt ccataacaga ttggctaact cctcctcttg
    13141 cgaagtatgc agtatagtca aatcctggtt tatcttttaa aggtcctttt tcaccatatc
    13201 ctaatatttg agagaatatt aatcctggat acttatcttt tatttggtca taagctatac
    13261 ccattttttc taatgcttga actctaacat tagttacaaa tatgtctgct tctgataata
    13321 atttatgtaa tatttctact ccttcttttg attttacatt aatacttaca ccctttttat
    13381 ttccattttc taattcaaac atagggtttt catcatctga tgctggagat ttaaatgttc
    13441 cacccataac tcttattcca tcaccttcta taggttcaat cttaataacc tctgcacccc
    13501 agtcacctaa catttttgca caacatggtg ctgcgatgaa acttgaaagt tctactactt
    13561 taactccttc taaaagcata acgttttcct ccttaatagt atcaattata aaatttcgat
    13621 taaatcactc ataaaatact tttttctgat attaactgtc tgatgtcaaa gttcctaatc
    13681 ttatctaaaa taacttaaaa attattaaaa ttatttttta ttgttttatt gtcttacacc
    13741 tatattgtta agcatttatc gtgccaactt tatatttaat caaaatatcg cttgatttga
    13801 atgtttttta acaatatgca attgaataat ctttttcata tcgtggcatc ttgccactat
    13861 ccattgcatt ttgctattct ttctatgctt ataaaagttt tttttatata tttttttatg
    13921 aaaaagcttg acatttcaaa aaaagttttc attgctaaac tcaaaaacac atgttatcat
    13981 tgtaattaat cagcaagtta gttaactaat ttcaaactta gataattttt taataatatt
    14041 taatctaaaa aataacaatt tctttgataa attttagagt aattattaca ttaaataaaa
    14101 aattactaaa tatcaaaata acacagggaa aacaatttca aaactaaatt tttaacctta
    14161 aaaattttgt ataaaaaata taaccttata tgctacatat tatttctaaa tgaaaaatta
    14221 ccataataat ttaataatta caggagggta ttgatatgaa aatactagta tttggagcac
    14281 gcgattatga agaaccagta ataaaaaaat ggtctgaaga acataaggat gttcaagtgg
    14341 atatttatcc tgaaaacatg actgaagaaa atgtagttaa agctaaaggg tatgatggta
    14401 tatctataca acaaactaac tatatagata atccttatat ttatgaaact ttaaaagatg
    14461 ctggggttaa agttatagct tcaagaactg caggggttga catgatacat tttgatttag
    14521 ttaatgaaaa tggacttatc gttacaaacg ttccttctta ttcacctaat gcaatagctg
    14581 aattagctgt tactcaagct atgaaccttt taagaaagac tcctctagta aagaaaaaag
    14641 tctgtgaagg tgattaccgt tggatagctg aacttcttgg aacagaagtt agatctatta
    14701 cagttggtgt tataggtaca ggaaaaatag gtgctacttc tgcaaaatta ttcaaaggcc
    14761 taggagctaa tgtaattgca tttgaccaat atccaaatag tgatttaaat gatatattaa
    14821 cttacaaaga ttctttagaa gaccttctaa aagaagctga ccttataaca ttacatactc
    14881 ctttacttga aggaacaaaa catatgataa ataaagatac tctagctata atgaaggatg
    14941 gagcttacat agtaaatact ggccgtggtg gtttaattaa tacaggggat ttaatagaag
    15001 cactagagtc aggaaaaatt agagctgctg cccttgatac atttgaaact gaaggattgt
    15061 tcttaaacaa aaaaatgaat cctggagaat taactgaccc agaaataaat aaacttcttt
    15121 ctatggaaca agttatattc actcatcacc ttggtttctt cactagtaca gcgattgaaa
    15181 atatagttta ttctagttta agtagtgctg tagaagttat aaaaacagga actgctacta
    15241 atagagtaaa ttagtatttt ataaatatat tttttcatta attagctatt atctaacaaa
    15301 atagccttta atttcccaaa tataagagag tcagctcagt atatattgag tagactctct
    15361 tatattattt tttatattaa acttgtactc ttcttattca taccaaatta aatttatatt
    15421 aacttattct aactactcct actctataca ttttacctat ttttactttt ctaattatat
    15481 acatttgaat gggtacaatc tgtattttac aaaaaaaaca cctaacaaac tatgaaaatt
    15541 gtatgattaa tattaatata tttataagaa aacataacaa ttatgttagc tctggatttt
    15601 tagggaggga gtatattgga aaaaatgaat aaagttactt ctaaaaaaac tacctatatt
    15661 gttacttcag cattatttgc atccataata tgcctgacaa tagcatatat attacatatt
    15721 ccagttggtg gaaacaatgg atatgttcat ataggagatg cttttattta tctagctgca
    15781 actattttgc caacaaacta tgctatagct gcttctgcaa ttggtgctgg tctagctgat
    15841 ttatctacag gtgctgctat ctgggtaata ccaactataa tcataaaacc aatacttgta
    15901 ttatttttca cttctaaaag tgacaagata ataaataaga gaaatatagt tgcttcggtt
    15961 gtagcaggta tagtaggatt agttttatac atgtttgctg aaggtataat tattggaagt
    16021 tttacaagtg catttgtaat gagcttactt ggcttattac agccaatagg aagttttatt
    16081 gtttttataa tcttaggtat ggcacttgat aaacttgatt ttaaaaaacg ttattttaat
    16141 taagaattta aatttatgga gggaaaatta tgaatatctt atatacaatt gaaaatagta
    16201 tctatgtaaa tataactaat acatgtccat gtagttgtgt tttttgtata agaaacgaaa
    16261 aagatgaagt tgcaaatagt ggtagtttat ggttggagca tgagccaagt gtagatgaag
    16321 taaaagaagc cttcaataaa tacaatttag acgactatga tgaaatcgta ttttgtggat
    16381 atggcgaacc attaatgaga ataaatgaat tgatagagat agcaaagttt ataaaagaaa
    16441 aaagtagcat aaagataaga ataaatacaa atggtctaag tgatttaata cataacaaaa
    16501 aaacagctat tctgttaaaa aatgttatag atgcagtatc aataagtctt aatgccccaa
    16561 ataaagaagc ttataataga gttactcaac caaagtttgg agaaaaatca tttgattata
    16621 tgttagattt tgcaagagat tgcaaaaaat atataaaaga agtagctttt tcagtagtag
    16681 atgaaatatc tccagaagaa atagaagaat ctaaacaact ggcaaaaaaa cttgatatac
    16741 cattaagagt tagacataag aattagcaag taaaattata aaacattaag aatcacctga
    16801 atggtatttc aggtgattct tcctcttgta ttaacatttt acaaaataga tttcatacca
    16861 cactaggaat gtgtaaatca tccaaatctt tctccaatta tcagagtttc cactaatgta
    16921 gtcattaaaa atctcattaa tctcatctat gttaaagaat ttatctgcta tctcactatt
    16981 gaatttatct cggatatcct tattatatcg gtcatcagcc atccatatac gaattggcac
    17041 aataaatcct aatttatttc taaaagcaat ctcttctggt aatgtttttg atgcagctgt
    17101 acgaaaggca attttattct cttttccttt cactttatat tctgatggta ttctggatgc
    17161 aatatcaaat attcttgtat ctgtaagagg cattctgact tccaatccta ctgcagtact
    17221 cattttatct atattaaaat aaatatctcc ttccaaccat atttgaatat ctacatttga
    17281 catgttagta agtgtatcta gttcttctgt ttcttcataa attcctttta ccacttcaat
    17341 aggaagcaca tcaggattgt atttcttaag aatttttctt ttctcaccct cattcataat
    17401 gtttgtattt ccaatataaa atgtcttcaa attctctcca taacactctg ccttatggta
    17461 tatattatat ccaccaaaaa actcatctgc tccttctcca gagtaacaga tatttgtgtt
    17521 ttcagctgct gccttgcatc caatagcaaa aacgatagca gatgcatctc cagttggttg
    17581 ctccatatgg tacattacat aaggtactga cgcaaaatac tcttcaggtg taattatata
    17641 tctcgaattt ttacacccaa gaatattagc tgtctgttgt gctaattttg attcatcaaa
    17701 acgctcttcc tcataaccac atgaacctgt tttttctata tctgacattg ccaatatata
    17761 agatgaatca acacctcctg ataggaataa ctctgcagtt tcattctctg attttacctc
    17821 tggcataatt ttctgaagtg ttgtatgaat ttcatccgtc cattcctcaa tagacttgct
    17881 gtggtcagga tgaaattcag gtttccaata acgaccaata catactgttt tattctgata
    17941 gacaaggtaa tgtcctggca taagcttctt tactccacgg aagaatgtat tttcacctgc
    18001 tacatatgtg aaagacatat aaatctgtaa catgtcttca tttaactctt tcacaaatcc
    18061 tggttgctcc ataatcttac gaatagatga accatagaga agcttcccat tttcagtctc
    18121 ataataatag aaaggtttta ccccaaactg gtctcttagg cagaagagtt tcttttccac
    18181 agtatcccat aatgcaaaag caaacattcc atgcatgtga tttgccatat catatcccca
    18241 tgtcttatat gcttccacta aaattgctcg ttcacgctca tctctacata atgaagaatc
    18301 gattccaagt tcttcacaaa gaactttcca gtttcgaata tgcccattgt attctagaat
    18361 ttttatcata tatgtgtctt ttcgtgttaa atacttttct tctaaattta tcatatgtgt
    18421 atcctttcgt attaaaacat ttaacttcta aatttattaa atgtttatat atatttattc
    18481 acttataatc actgaaaaat cgactttttt caaagtaaat tctttttgaa ttagtactaa
    18541 tctgctcttt attaatggca taaatatgat tatcaagtat tatcagtgtt ataacattga
    18601 attttttatt cttcataaat attgccccct ttatattacc aactaaatat aatatatctt
    18661 atgttactta catattcttt aagaaattct gaagatttta taaagttttt tgtattataa
    18721 ttaattaaaa ataaaattct agttataaaa catattttcc attaaacaaa aaaatagtta
    18781 aaataaattc ataaccacat aaacttattt taactatttg tatattttaa ttataaatat
    18841 taacttaaat ttttaacagg atacactcat aaaataaaca taatgaacaa caaaataata
    18901 tgtctcagtt tctttatata ctttaccttc tttcaatttt ctaccatgta gacgtataag
    18961 ataatattag taattcactc tattgcttaa ctcgtgcgat atgtacaacc aagaaagcta
    19021 tttcctcatt tgttaacttt tttccatatt tcttttctaa aaaatctcta acttttaatg
    19081 caattaacat ttcttttgga tttgaccttt tcatttgatt aaataataaa tcatcaccac
    19141 tgcttaacat actatctgaa aaaaatcttt ccacaaaaaa ttttatatgt gttataaatc
    19201 tcatataatg catactcttt ttatctaagg ttctattcaa agaaaaaaca aaaatattaa
    19261 ttatttctcc aattactttg gcatacttaa tcgtatcata tgaatttcca tctgaagcca
    19321 tagcatttgc aaaatggaaa gcaatgttag ctgcttcttc ctctggtaat tgaatgccca
    19381 atctctcctc taccatataa attgcaacca ttccaacctt aaactcattt ggataataat
    19441 tttttatttc ccaaaatact cgatttgtaa tttttattcc tttacgcatc ctttcaattg
    19501 caaagttaaa atggtctgct aatattaagt ataagttttt attaaaagat gagttcaata
    19561 acttttctgc ctctactatt atttcctgtg ttatctttaa aatgtcgaca ggaatatcat
    19621 ccattaattc taaaaattgt tttgattttg tattttcaac agggacaaac atttgattat
    19681 cattggaacc acaaatatat tctcctgttt ttttaccata tccaattcct ttccctagca
    19741 aaataaattc attatacttt gcatcatgaa ctaaaacaac gctcgagttc aaaactttct
    19801 ttattatgta gttcatttta tttgctcacc ttctattggt catttaaaat ctatactatt
    19861 attttcaatt acctttttat accaataaaa actatctttc ttatatcgat tatatgtccc
    19921 ctttccatag tcatcagcat ccacataaat atatccataa cgttttgaca tttgtttcgt
    19981 tgattcactg accaaatcaa tacaacccca tgaagtatat ccccaaagtt caacgccatc
    20041 ttcgataatc gcatctaaca tacatttaaa gtgttctttt aaataattaa ttctatattg
    20101 gtcatgaact tttttatctt cagttaaaat atctttagct cccaatccat tttcaactat
    20161 aaataaaggc tttctgtatc tatcatataa ttcaatcaaa gatatacgta gacctattga
    20221 atcaatttgc caaccccatt cactcatttc taaataagga ttttttacag ctaataacgt
    20281 attcccaggt gtaatatcta atccagttgt atcagctgcc tcacaagaag acatatagta
    20341 agagaatgaa ataaaatcta ctggatactt tttcataatt tcatcatcat gttcttcttt
    20401 tactatttga atgttattat ttttaaaata cgacagtaaa tatactggat actcaccaaa
    20461 aacctgcgta tcactatatg cataaatact tctcattttt tgttgagttg ccaaaacatc
    20521 ttctggtcta catgtatatg gataatacgt taattttgtt aacatacacc ctacttttga
    20581 atttggaata atctcatggc aaatttttgt tgcaatggca ctagctacaa attgatgatg
    20641 catagcttga aaaataactt cctcaaagtt tttatcctta aaacggtctc gaactaaacc
    20701 accagtagtg tacggatgac gaatcataga atcaacttca ttaaatgtaa gccaatattt
    20761 cactttgttt ttatatcttt cacagatagt ttctacatac ctaacaaaca tattgataac
    20821 ttgtcgtgaa taccatccat catagtttaa aacgatattt aatggtggtt cataatgtga
    20881 catagtaaca agtggttcaa ttccataact tatcaactca tcaaatacat catcataaaa
    20941 ctgtaaacct tcttcatttg gctctttatc atctccattt ggaaaaatac gagaccaagc
    21001 aatactcatt cgatatgtct taaatcccat ttctgccatt agagcaatat cttctttata
    21061 gtgatgataa aaatcactac catgacgttt tggataattt acttcatctt caggatgtgc
    21121 taatccttct tctatatctt ttgttgtgat ttcattatta gatttataat ctttcacatc
    21181 tgcatcaaag tgtgcctttg ctacatctga tacagaccac cctttcccac ctaaattcca
    21241 accaccttca aattgattag cagctgtagc tcctccccaa agaaatcctt ctggaaattt
    21301 tctcatattt atattcctcc ctttacatca aacaagatac ttcctttttt tacattgcca
    21361 taagactggt ttaacacttt ataagcataa tctgctccat tattgtttac aatcataaca
    21421 gttgaatcta aattagcatg tgagattttg tctaaatcaa attgaattaa taaatctcct
    21481 tcttttacat tatcgcctgt cttaacaaat gttttaaatc catctccatt catacttaca
    21541 gtatttattc caatatgaat caaaatctca gccccatttt ttgttcttaa accaattgca
    21601 tgttttgttt caaataacat cacaacttct gaatcacaag gtgcgtataa atttccattg
    21661 gttggtataa ttgcaactcc atctcccaaa gtcttacttg caaatagttc atcattaacc
    21721 ttacttaaat ctattacttt tccttctact ggagttacta agatatttac ttctttttct
    21781 tcttctagct cttcttcaac aacatctcca attgatgact ctactgtatt tgtatcttcc
    21841 ttccaaataa tcagtgtaat aataaacgag cctaatattg caataacaaa accaatactc
    21901 gcaaaaatca aattgtttcc attgtttggg tcaataaaca tactcatact ggcaagccct
    21961 ggtccaacaa ttacaaatcc ttttactgca aataatccta aaaaggtacc agaaattgca
    22021 cttgagaata caacacctaa cattgctctt ttatgttgta aggtaatacc atataaagct
    22081 ggctctgtaa ttcccatcaa ggcagaaatt cctgcagata atgctgtttg ttttaatgtt
    22141 tcatcttttg ttcgcaaggc tactgcaaaa caagcaccac tttcagaaat attgtgggct
    22201 aatgaagatg tcatatataa agcttcataa cccaactttc caattgtatt aattgaataa
    22261 ggtattagtg ctttgtgcat tcctgttgca accataaatg gaagaaaagc agcaagtaat
    22321 cctacaacta agaatcccat tttgttatat aagaataaaa taatggttgt aaaaattgta
    22381 ccaacgttat atcctaaagg acctaaaata gttagagtaa ttggaacagt aattgccaaa
    22441 gacatcatag gaacaaagaa agttcgaatt ggttttggtg aatacttgtt aaaaattttc
    22501 tccattgttg ccaagaaaga cacacataag atagccggga aaacctgtgc attataggca
    22561 atgtttggaa ttgtaaatcc aaatagttca acaccttcac ttaaagcagt tgttgttgct
    22621 ggaagcaata agtatcctac agcggcaata gctactaaac gattacattt caatacattg
    22681 gcagtagtta cagctaccat aagaggtagg aaatagaatg ttgcatctga aatagcaact
    22741 aataatgtat atatagtaga atctgatttt aaaatattaa acattgctaa taacatcaac
    22801 agtgatttta aaataccagc tcctgcaata gcaaaaatca gtggttgaaa aatattaata
    22861 ataaattcta taaatcttgt tcctaagttt attttttctt cagtttttac agtactatca
    22921 tttgtatcta aagggcaaac ttttaaaatt tcatcataca catcaacaac actatttcca
    22981 atgacaactt gaaattgtgc cccattaact atactcataa caccttgtac tttttttaaa
    23041 gcatcttgat tgactttact tctatcaatc aacttaaagc gtagtctagt tgaacagtgt
    23101 tctaaagcta aaatattttt tgaaccacca acttgttcaa taatgtcaat ggcagttttt
    23161 ttataattca tcttgtaatc tccttctcca taatttttta tgaatggcca ttcaagatgc
    23221 aaaaaaggca gaacctatat aaacaccttg aaaaatcaaa gcaatatatt cgatcctgcc
    23281 tgcattacca gtaacatgtc ttctatttat actattttgt attattatat taacatttgc
    23341 ctaaaagaat tgtcaatact tttttaaatt ttctgatact aactaatagc acctttataa
    23401 ttcatttttt atgtcaatta aagtaacagt agccacccac caattagtat caaaattgaa
    23461 cctggtattt tttcttttat attttctttg aatataaaat gagcaagtat taaagttatt
    23521 atcatagata cttgagaaaa tggctctgct atactaagtt ctagattatt taaagcaatt
    23581 aataatgcta aataagaata tccattaata gcaccactta aaattgaaag ccccttttta
    23641 ttagtaaaaa tctcacaaat tattttttgt ttctttttaa atgctaaaaa gataaataaa
    23701 tttaaaccta cgaaaaaata cagtatagtt gaatataata taggagatac ttgtacaaaa
    23761 aaagccttat ccaatactct tcctactgac tgcaaaaata tgtataaaaa cattaatcta
    23821 catggtaaat tgttaaaaat tacagagaca ttctttaaaa aagaacttac tttttttaat
    23881 ataaacactc caccaatcat aattatgatt cctgcaactt ttgctaaact taaaccttca
    23941 cctaaaaata taactgataa aagcagtaaa aacaatgaat ttaaactatt tattggagta
    24001 acaagtgaaa cttccccagt tgctaaagac gttacatacg ctactgaacc aattgaatag
    24061 actaaactac ttatataaca tggtattaaa aacgaccaat cactaactct atccattatg
    24121 ctaaatggaa gtaaacatac tccccctata aagaaaaata gaaatgatga agctatattt
    24181 ttataaatat ctccttcttc atcacctaat aatcttacaa ctactctttc aaaagctaat
    24241 agcactactc taattaataa tgcaaaataa gataatatca tttttgacct cgcttaaaaa
    24301 ttaatccgac ttattgttat attaactgta taagactacc atataggtaa gtcttttgga
    24361 ttttttaata ttttatgttt aaatacaggt acattaagtt tttcacctag tttacggcat
    24421 tgctctttat aggtaccttt taatttatca tgaaaaacta tacaatcaat tcctccttca
    24481 atgctattta tacacttttc tagtttctca aatccagatt catcatcata cattccactt
    24541 acaatccatt gatttggcaa tacatgtcca attgatatgt tatattcttt attttttata
    24601 atatctgaga aagatttctc aaaatgtacc cctccaacac ataacaaagt cttcaaaggt
    24661 tcatcacaat caaatactct aattaatgat tttgccaata cctgtattgc tatactatta
    24721 ttaaaactat cactactact tcctatctca atatccaaca taggtacaga atattctgta
    24781 attaatttag attcttcacc atatgttgtt ccagaccagt gagttgcttc tgttaatgta
    24841 gtaaattcat caagtagatt ttcatttttt atatcctcaa tagcattgat tagattttta
    24901 aagtatcttg gatttgaatt tccaaattct cctgttggaa catctccaat tgtatgagct
    24961 gttaggatat gttctggagc attttctccc tcatgccaat ttacaactcc tgcaaaatca
    25021 taatctctaa aatgtctatt taaaattgga agataatatt tataattatg acttattaca
    25081 tcttttgttt ttacaaaatg aaataagtta ttgtttttat ccttaaactc taatacaaaa
    25141 ttatcatcaa taacaatatc agtttgattt aaatccatta tttcttcaca acattttatc
    25201 acattactgg ctactgggtc tttctttaaa tcactgcaaa agaaaaaaac tgctttttta
    25261 ttattcatcc ataaatcaca ctcctataac ttaaatataa tatatcttca tacctaaaat
    25321 catctaaata tcactctaat tccattaaat tatttatata taatatacta ccatactcat
    25381 tttagctata cctttgcctg agtttctata tatatgtgat acatcagctt taaatcttat
    25441 agaatttcca ctagatacaa tgtaatcttc tccatttatt ttaatggtta cttcgccact
    25501 aaaaacagtt ataaactctt gtgttcctgg aagttcagca tttgaatcta aatttccacc
    25561 ttcttctatt tcaatcataa atatctcaaa tcttcttttg gagtcaaatg gaaatattgg
    25621 ataacttata tactttccat tatcttcaat aaaatggtct atatcacttt tttgaatcaa
    25681 ttctatgtca ctttcctgcc tctccatcaa tgatgtaaat gaaattttta gcccattagt
    25741 tattttcttc aaagtagata tagtaggatt tacctcacac ttttcaattt gagatagcat
    25801 actcttactt acaccactaa gttgtgcaat cttgtctaaa ctaagatgtt tttctttcct
    25861 tatctttttt aaattttcag caattatttc atttaaactt tgcataaata ccccctaata
    25921 catactaata taagataaat tattttgatt attaaataaa cttagtaatt taatagaaac
    25981 tttttatcca ttataatgta cattatgttt aatatacatt acttttttta gaatgtaaat
    26041 agttttctga aattttgtga aaatgaataa ataaaaaact agtaacctat atttttacaa
    26101 ggttactagc ttttattata ataatatttc attttaacta taaaacttag atgcacctat
    26161 aagctgtctc ttagaaacaa tcttagctag gtttgctaat gaagaaattt ttatatcttt
    26221 aaaagaaact aaaaatacaa acttaaaatc tgtaaattct tgatttatat tgtatatatc
    26281 aaaataacat ctcaaattat caaataagta attatcctgt aagtctgctt ctatctcatc
    26341 agtagcatct tttaaatacg ctcttacatc ttcaaataaa acactgaaat aagactcatc
    26401 tatttcttca cctaactctt ccaatatttc ttctatatct aaatcatcca tatcaaaaga
    26461 atttcctgtc aactggcaat caaataccaa tatattttct tgatttatag tatttgatat
    26521 atcttgtaca tattcgaagt ctggacgtaa ttctaaaatg tcttttattt cactgctctt
    26581 tacatggatt tttccttttc tattttcaat ttttaaatct atcatatgct ccccctttca
    26641 atctacaaat ctttaaatac cttgttttat aatcttttca atatctatcc aattactaac
    26701 tctaactata ttttgatgtt taacatcttt attatagtta gtatccagaa gtattacttt
    26761 aacaccatca tcagctagtt gcactgagtt taaaggatta tcttctataa atatgtcaca
    26821 actcagttct cttgcctttt caactttata atcacttcct aataagtaaa catctatatc
    26881 tgaaaatcct tgtctcgata gccaattttt agttacatca gttaaacact catgtctagc
    26941 cgttacaaaa gataaattat gtcctttaga taatgtgcta attacctctg tagcaccctc
    27001 tacaatcttt gcttcatcat atgagtgtat atacttatta ttaaactccg catacacatc
    27061 tcttattccc gaaccataca attcttccca attatgtgtt atacactctg cttcagttat
    27121 atttttatta aagatatcat ttaggtatgg aagaaaatga taagggcttg ttattgttcc
    27181 atctatatca atacatatat tcaatttgtt catttttacc tccatgtaac atttattgat
    27241 ttgattaatc aaatattata gcacagcttt aattataaat caaaactctg gatatgacta
    27301 tacctatata ataccaaaaa gcttgcatat ttaagcaagc ttttcgtaaa ttttacattt
    27361 ttaactaata tataagtttt tctaaggttg gttccactcc aaattgtgat ttcagttctt
    27421 tatattcttt taatgctttg tcattattcg ttttagtttt tatcgctctt atcattaaat
    27481 tttttggtgt atcaagtgga gatatgtatt ctactacaga agtatcatat ccattgccct
    27541 ccaataacaa tgttcttaaa ccatcagtta ttaaatctgc aaatctagct ttaaatacac
    27601 catgttttaa tattggttcc atagcttcat atctatattg ccctaataac tccttatgac
    27661 aacatggtac tactactata gcttcagatt ttgctctaat tcctagacct attgccatat
    27721 ctgtagctgt atcacaagca tgtagactta ttacaaggtc aacatcccta ttaggtgtat
    27781 aattagtcaa atcctctttt ataaatgaca tatttttata acccaagttt ttagccatat
    27841 tttttgatgc ttctataact acatctgaat aatctattcc tataaaataa cagtttttct
    27901 tcaatacttc ttttatataa aaatttaaga caaagcttaa gtaagatttt ccacaagcac
    27961 aatccaagat agttatacag tctttatcct ttatttcttt taaaatacta tctatcaatt
    28021 caacaaaatg gtctatctga ttgtattttc ttattttatc attttttatc ttaccatctt
    28081 ttgtaagtac tccaatttct tttaaaagtg catttgcttg tcctactttt acataataat
    28141 ctctgttttt tatttgagat gccgtaaatt catctatttt aggtacttct tccttattat
    28201 ctaaatattt tacatttacc ttcttattgt ctccttcaat tacaacaata gttcctcgct
    28261 caacatattt aaatactaat ccatcataat tttctgcctc tttagaaata ttcttaagca
    28321 actcattaaa cgtatataaa tctgattttc ctttaaaatt aaatttatat ttattatctt
    28381 cctctattgc aattccttta aactcttttc ttccagattt aaaaatcacc tctaactcaa
    28441 taaaaaaatc cctattatca aggtttcttt gagtaatccc cataaaaaac atatttagtt
    28501 ttcctatact ctgtttattc atcataatct cccttttaac ttatatcttt aaatcttatt
    28561 aattaatatt atttgtttct gaattattta tttcttcttt taaatcgttc aatctatttt
    28621 taaaactttc tatctgctta tctgataaat caagatattc tgttatcttc cctatatatt
    28681 tagggctttt agtatttatt aatttcaaag cactttccat agatttttct ctaagctcat
    28741 atgctttctt tacgtcatct ctagcattac tcaatgtttc tgtatacttc tccttagaaa
    28801 gttctggaat ctgcatattt tcatattctt ttacaacttc tttacacttg tttctggcat
    28861 ttgtaacatc tttttttata ctagataatt tagttatatt ttttgctata tccttagtgt
    28921 aaggtataca ttctctcatt ggaactaaag ctgctctttc tatattataa atttctttct
    28981 taaaaattcc tatactttcc atatctattt tttttatatc ttcactattt ttttctttac
    29041 ttgtatattt attattttct tttgtattat cttcttgttt ttctgattta ttattcactg
    29101 aaacattact atcagaatta ttcttttctt ttatatcatt acttccttct tcattacttt
    29161 ctttattttg tatatttcta tcttgtttgt tgtttaaatt tttattattt gatatttctt
    29221 cattcttttt attatcctgt tttaaatcag tactttcttt ttccttaaaa tttgatacat
    29281 acttttcatc atatgtctca tcagaaatat aattgtgata atacatagac tcttttattt
    29341 tattaagtcc actcatataa atattactta tcattattgt tgtagctaat accacataaa
    29401 tactactgta gatacaagac ctcttaaaat tcttgtaatt tctgaatatc actactaaag
    29461 gaataagata taaaaatata aaaactaata tccacctcat aagtcgtgtc tcctttctca
    29521 aaaccgaaat ccctcaaata tttattttta atcatgtatc ttatagtatt taccaattta
    29581 tgttttttaa tcgtgaactt ttattaaatt aaattctgta tagtcacaag atgctaatgt
    29641 atattctact ccatttcgaa tccccttcaa tcccatttta aaagattctt taccatgcaa
    29701 gtgaccataa atcacctttt caacattata ttcttcaaat aactttgtaa ataaagattc
    29761 ttccaattta tcattcgtag gaggataatg agtaatcaca attatttttg agtgaccact
    29821 tttacgagca gattctaatg atagtctaag tctatgttct tctcttttat aaactttttc
    29881 atcagtctca tcaaatttaa catcattagg acatatccag cccctacctc cacaaattgc
    29941 ataatcttta tattcataaa aatttgtctg aataaaacgc atgtcttcat atagcttatt
    30001 taaacttgta acggttgtcc accagtaatc atgatttccc tttataagta ttttctgacc
    30061 tggaagtttg cttattatat ctaaatcttt tttagcttcg gataaattaa ttccccaaga
    30121 tgtatctcct agtactaaga ccatatcttc ttccttaaca acttctttcc aattatctat
    30181 aattttcttt tcatgaccat cccaattact tccaaagata ttcattggct tattgactga
    30241 tgttgaaaaa tgtaaatcac ctatagcata caaactcata atatcattcc tttcttaatt
    30301 acactaaata ggctgtctct ttttagaaat tattttccat taagaggcag ccccattaat
    30361 atttagcaaa ttttctacaa gtagttttta ctgaactact ttatcatcta ccttagtatc
    30421 cttatattgc tctaataata tatctatttc ttgttttaat tccaaaattt cagttatatt
    30481 tttatctgtt tcagttctat caagttttct ttggtactgc atagctttat gtgtatcacc
    30541 caagtcatat aactcttgta ttctatttaa agtatctcta atagctatag acttcatttg
    30601 gtacatagcc tgagctttca ttatgtcctc tcttatttca ctcatttctt ggtctaaaag
    30661 aaaggcagca tcagatgctc ttctaagcct ttcagcaaga gtctcaggta tgtaatggtc
    30721 atatttatat tttaatgaat gttctattgt agcccagaaa ttcattgcaa gagttcttat
    30781 ttgtatttca catataattt cctttgaacc cgcaatggag tttatagggt attttattat
    30841 aacatgatag cttctataac cactatcttt gtaatttgta atataatctt tttctcctat
    30901 tatagtcata tcatttctta cttttatcaa atcaactata gcatatatat cctcaacaaa
    30961 ctggcacatt attcttatac cagcaatatc atcaatttca gtttctatat cttttgcatt
    31021 tatccttttt aacttagata ttatggaaga tatttttttt gttctacctg tgacaaactc
    31081 tatgggagaa tattcacctt ttgctaaata ttcttttctt atatttttaa attttatttt
    31141 caattcctct actgcatgtt cataaggtgc tagtatctca ttccattttt cgtattccat
    31201 attgctcacc ctttatttgt tttttatgac cttctttatt ttatcataat ttattttgtt
    31261 tttgtatagt aaagcaattc tccattttcc aatatatacc ctatttcttc taaatcaact
    31321 aagtaactta tgacagatac taacgaagat ttaaaaaaat gatactcttt ataattattg
    31381 cttaaattat tgttatttat aatgtttttt aataaaattt ctaaagattt cggttctaca
    31441 agcaactctc ttatttgata taaatatttt tttaaagcat tcaaatgttt ttctattaat
    31501 ttataagaat cttctttcga tataacttgc ttgccatgcc ctaaaactaa gtactcaaaa
    31561 tctatatttt ttaatttttc taatgaattt atttgttctt gtatatcata tagaaacaag
    31621 aaatcgtatt ttgatagaat ttcatctcct atcaataaat cgccaacaaa aatcacatta
    31681 tccttcgtca aaacagctat actaccaggt gtatgaccct gaaattcaat tatatcaaac
    31741 atttcattgt ttaattcaat ttttccagct tctatagatt tatctatttt tatatcatcc
    31801 aacattttat tttttaagat attatccata aatacattgc tctttccccc aacaatatac
    31861 tttgaaaaca attctggatt ttctatatat aatttatcgt attcagatga cattatacac
    31921 acatctttat aatattcttt aaattgatta caagctccat agtggtcatc atgaccatga
    31981 gtatttataa tatatctaac ctgtatatta ttttcatcta atactttatt tactctccta
    32041 ggtcttgaac cacataaccc tgggtctatc ataagtgctg tgttatcttc aaatacatat
    32101 attcctgtat tcgtgccacc ttttataaaa aaggtatttc cttttacttt tgtaagattc
    32161 accttcttca cctcattttg acaatattta tgatacaatt tattacttac taatataaaa
    32221 ctatacaaga acaatccttt ttattctatc tatagttctt tttcttattt tgtattcttt
    32281 agatatcctt attttcacat tatatgctaa atttctataa agtaatgact ctataaaata
    32341 ttcaactata cctctatccc taaagtctat atctatattt ccacttgaat aaatatttga
    32401 gtctaatatc ataagctcat caaaaaagct taatctctta gatttactta aataatcttt
    32461 atttggaata aatctatcac aataatctat cctgtaattt actacttcat catttatttc
    32521 aaatccatat tgctttttca ttaactctct atagttatta tttttctcaa taacattatc
    32581 taaattttta ttataatcct cttttttcca atgtccaaac atcctacaat ttaatggtct
    32641 aacttcatat attaaacatc tattattagt atctttaaat ggacatgggt tctttcttgt
    32701 atactcctca aaataataat ctaatatttt acttatacag ctttttttta aatcaactct
    32761 atcttcaaga taacaaaata tattcaagaa ctcaattaaa tttataccta ctgactccat
    32821 acaacaattt ccacatccag aacaatttcc tactggcaag gacttatata catcatttag
    32881 tttgtcaaag agcttatttt taatggaata atctatacaa tttaaaatat ctttattttt
    32941 tatactagtc attattatta ctccttgtta taattattaa gttaatacaa acaattatta
    33001 taacatattt tttaaattta atcgcatatg gaggtatttt atgaatagat atacaaaaat
    33061 aattaatatg atgggaagtt attacacaaa agactttgaa aaagaaaaaa agaatgtaat
    33121 aaaagttaga gaagttaaag aagatacagt tagaaaattt ttcttacaag gtgactgtga
    33181 agttcttgta gtatttgaag acactgggaa agaaatactt attgatgact tttctcctga
    33241 agaagatatc aaaaaatatt taggagcaaa atttataaat aaaaaaagat agttatcaaa
    33301 aataattcta aaattaaaat ttaataaatg tacagttata attctatata attatagtta
    33361 aaagttatct tttattgctt ttttagactt taagatagct ttttttctat tttttgtaaa
    33421 ataattccat aattcacatt aataattcac aaaatgatat aatataacaa atagataaat
    33481 ttttaaattt ctaaatacat aattaatatt ttgcgaggtg attttatgca aatttatttt
    33541 agagttaagg ctgttggcaa acgcaaacct atgctagaac ttaccccttt tgatgtatct
    33601 gacaacatca attgcctaaa agaagctatc tctgaaattg tatctaaaaa tgttcttgaa
    33661 tataatgaga aaatcccaga aaaatcaata ataaacttct taactaatga agaaattgaa
    33721 tctcaagctg atattggaaa agttggattt ggctctattt acaatgagaa taaacaatct
    33781 gtagaaaagg caatagaggt agcgttacag gcatttgaag atggaatcta caaagtgctt
    33841 ataaatgagg atgttattga aaaactcgat tcctctataa atctgaaaag tggagatata
    33901 tttactttta taagacttac cttgcttgct ggaagaatgt tttaaaataa ataatttgga
    33961 ggtacataaa tgttagaacg tactagagta gaaaaaaatc ctaaaataat tgaaaatttt
    34021 aaaaataatt ttactaaatc ttcttcaaaa cagtactacg acttagcaaa gtctctttgg
    34081 gactgcatag aaaatagttc atactattat agagaacctg aagacaaatt cttaaatatg
    34141 ataaagaaat actacaattg ccactactac gaaattaaaa atatggacga gctcctttct
    34201 ggacctttaa aaaatgcatt agaattaata tttacttctg aacaagtaga agaactttgc
    34261 caattaatta aaagaaagat ggattataca tattcaggag gatattatcg taaagggtat
    34321 cgctcaaata aacttgtgga ttattttaga ggtattataa ggtttatttc aaactatgtt
    34381 gatttaattt attatgattt ttcagttata gattatctta ctcttccaaa taaggtcttg
    34441 tactatgact ctataatgag tgataaaata gcactagaaa tagacaaagg aaatcaaaaa
    34501 gtaataagtg ctttagaaga agtaatattt ggagaaaatc aaactgctct acttacaaga
    34561 caaatgataa ttggtatcat gaagagtcat agtgataaat gctatgaaat gctttctaaa
    34621 cttcttttgg cagccaaaca acaagaagga cttagacagc aaattgtaga gagtttagat
    34681 gagggtacaa tttctgcatc aatatacatg ttaaaaacta ttttggacaa caatttagag
    34741 cgatttagtt ctgttataag agccttggat acatggacag gacttggttt ttcagaccaa
    34801 aaacctggaa taatacgtaa atgtttagaa ctttcatata aatcattgac tgatgaaaag
    34861 ttccgtatag aatcaattaa tagcaatgat aatcttgaaa tttattttgc cttatgggct
    34921 actgcaactt acaacatata tgactcttac tctcttattg aaaacttact ttcttgtcag
    34981 gaaaaatata aaaaacttgt agctctttac tttttatcgc atacagaaag tacatatttt
    35041 aaacattcta tagcatctaa aaacattaag ggtgatgatt tagagatact agcatgggta
    35101 attacaaatt tctatgtaga aggttctgtt ggctactact atagttggca aaacagagaa
    35161 actaattttg acttgtcaaa cactcctatt gacaaagact ttgatgctag aaaagaacaa
    35221 tttgaaagtt taaaacatac tttagaaatt ttaaattcaa agacaaaatc attttcagga
    35281 agtgtatttc catggacttg gattgaaatt tcttctcatg aaatacttaa atgtatgatg
    35341 actttaacag catacgatat gaatgctcaa tttatagatg tgcttatgga aaattgtcat
    35401 cttatgagtt cagatttaag agcttcactt atagtcaatt ttgtaaatga accaaagaca
    35461 gatagacaac gtgaattttt attggaatgt ttaagtgata agagtgtttc caatagagaa
    35521 acggtattga gtctacttag aaagcttgag ctaacagatg tagaattaga taagataacc
    35581 aatctattgt ctttaaagac aagctctatt agacaaacag taataaaaat tttacttgaa
    35641 caagatgatt caaatttgga aaatattgct tcttcactga taaaatcttc aaattctcaa
    35701 aaatgccttg gaggtcttga aattgtaaac ttattaaaag acaatactgc tagacaagaa
    35761 ctatataata agcttatttt acttatagag gatattgata aatctaaaaa gaatacatct
    35821 caagttcaaa tactaattga taatttgtta ggtaaagcaa aaaatgatta ctgcaaagaa
    35881 aatggttttg gaatatataa ccctgaaaac aaagttcaaa ttccagaaca atttgatttt
    35941 aaagatgatt ctaatagctc atcaaagctt aataaaatta tctcaaatat aagtttatct
    36001 actcgtgagg attctgtttc tgtagcaaca aaaaaagaag caaaaaaaat tctcgatacg
    36061 gatataaata aaataataaa agtttatgaa aaatttgatg aacttattac tgaaaatgca
    36121 gattatgaat atgaagttga aagatggtca ggtaatgtag acaaagtact tttaggttat
    36181 tctacttata acttgactcc tattaaatat aaagtttcta atgatactct tgataattat
    36241 ccattagcag agctttggag agaaactagt aaaaaacttg acttagatta caaattaata
    36301 cttgatatct tattttatag tataagtaca tgtggatata atgaaacaga aaaaagttgg
    36361 ttttcatcta tatattcaaa tgtccttgac tctaaaaaac aaaataaaat atatacaaaa
    36421 cttgaaaaac ttaaatttaa tggacttaca agaaaacttt taagagtttt actaaatgag
    36481 tttgaccata aagaaatatt taactattgt ttagaaatat actataaact tagaaactta
    36541 ttgactatag aacaatgtaa aaaacaaaga gttgaattta ctaaaaacta ttacagacaa
    36601 aatgaatact gccctgcttt aaatgcccct attttaaact attggcttga taaagcacat
    36661 tcgtttattt atgatgatat ttcttttact aagtttttct acactacgtt ttcaaattat
    36721 cagttgctag aatataagtt tcctttctat actagcataa atgtgtttgc tcgtgcacat
    36781 gaattaggat taatgactga agagcaagtt aatagagaaa tgataagtgg atacaacgca
    36841 tcaaatataa taagaacatt gacaaatcct agatgggctg gcgacacatt agaaaagtac
    36901 ccttggctta aagataattt aaaaactgtt acaaaccgaa ttatagaaat tgaatcacgt
    36961 agaggtgata tgccaacaga agtgtctaat ctagctaata atatacaata ctttgaaggt
    37021 ataaattatt ttgtaaatat tttggtcgct cttggtaaag aaactttcca gagaggatat
    37081 tcatatggta gcaattattc aaaaaaagat gttttaagtt ctctacttaa aaattgctat
    37141 cctactaaag aagatgattc aaaaaaatta aaaagtttac ttaaaaatac cgacattact
    37201 gagttaagat tgatagaatc tgctatgtat gcccctcaat gggtaaacat aattgaaaaa
    37261 tatttaaact ggaagggtct gaaaagtaca atttggtact tccatgcaca tataaatgaa
    37321 ggcttctctg cacaaaaaga atcagaggtt gctctattct cccctatttc accacgtagt
    37381 ttcattgatg gtgcatttga tgtagattgg tttttagatt catataaaac tattggggaa
    37441 gaaagattca atatacttta caagtcagct aaatatataa ctagtggtgg taactctcat
    37501 agacgtgcac aactctatgc tgatgctaca cttggtaagc ttgataaaga catgttgatg
    37561 aaagaaattg aaacaaatag aaatcaagat aaattgagaa gtttctcttt acttcctatg
    37621 aaaaaaggag acctaaaaga agcaactttc cgttatgaat ttattcaaaa gtttgctaaa
    37681 gaaagcaagc aatttggtgc acaacgtcgt gaaagtgaat caaaagcttg tactatagcg
    37741 ctagaaaatc tagctagaag tgctggattt ggagatgtaa atcgtatgat gtgggctctt
    37801 gagactgaaa aactaaatga agttgctcat ctatttaaac caaaagatat tgatggtact
    37861 tcaatttgga tagatgttga tgaaaacgga aaagcatcta ctaaagttca aaaaggcaac
    37921 aagtctttaa agagtatacc aaaagctatt gctaaacatc catatgtact tgaacttaaa
    37981 tctgtccaaa aagatttaaa gagtcagtat tctcgtgcaa aacagagttt agaacaagct
    38041 atgaaaaatg aaatagtatt tacagttaaa gaaattgcca atatctataa aaatccagtt
    38101 atctgccctt tgattaaaaa tctagtatgg gtatgtgatg ataatattgg atatgtttat
    38161 aatgataagt ctaagttctt tatttgcaat atagaaggtg aaacttataa gttgacggca
    38221 aaaaataaat taaaacttgc acatccagtt catttgtttg aatctggtaa gtggagtgag
    38281 ttccaacatg atttatttag taagcaaatt gttcaaccat ttaagcaagt attccgtgag
    38341 tattatagaa taaacagtga tgaattatct gaaaaaacta tatctcgcag atatgctggt
    38401 caccaaattc agccaaagaa aactgtagca cttcttagaa atcagctttg gactgtcgat
    38461 tatgaagagg gattacagaa agtattttat aaagaaaata tagtggcaaa gatgtttgca
    38521 cttgcagatt ggttttcacc agcagatata gagcctccta ctattgaaac agtagaattt
    38581 tttgatagaa aaacttataa aagtttagaa ttagcttcta tacctccaat tctattctca
    38641 gaagttatgc gtgatataga ccttgttgta agtgtcgctc atgctggtgg tgttgaccct
    38701 gaaactagcc attctactgt agagatgcgt atagcaattg ctaaagaact tttattatta
    38761 ttaaaaatta acaatgtcaa ttttgaaggc tctcatgctc ttatagcagg taatttaggt
    38821 gagtattctg tgcatatggg ttctggtatt gtacataaaa aagggatggg tgctttgaat
    38881 atacttccta tacattctca aagtagaggt aagatattcc ttccattcgc tgatgaagac
    38941 cctaaaactg ctgagattat gtctaagata ctattacttg ctgaagatag taagattaaa
    39001 gaccctagta tcttaagtaa tttataagta taaaaactat ctctaaaata tattggactt
    39061 tcttaaaaaa attagaaagt ccaatatata ctatactgta atttatatag attaaatcat
    39121 aattacaaat aatttttata taaacttctc ttatacatta tctttacggt acttattata
    39181 tcacacaaaa agtatttttc ttctatcttc actagaatct tttaacccta taaaatctat
    39241 ataaaaaatc tttttctaga gatttgttta atatggcatt ttcttgaaaa tttataaacc
    39301 ttatctctaa tgaagattta tttataggta taaccttatg tataaatctt agatttacta
    39361 tgtattgtct atgggtcata aagaaattag gaggtaggcg tttttttaaa tcatcaaagt
    39421 tctcataaaa ctttattata tcttcatctt tagtgtgtag attaattgct cttgaatttt
    39481 tctcaaacat aactatatca tcaaaattaa tcatatgtat ctgcttcctt attttatata
    39541 caaaaagggt gtcttctata aaattattat tagcaatctt atgggagtta atatggtcta
    39601 ttgctatgtt aattgaatca agtatttttt cttttttaaa tggtttaact acaaaatcta
    39661 taggatgcac tttaaagctt tcaagactat aatcagcgaa acccgtcaca aatactatat
    39721 gtatatcctt atctatagaa agaatttctt cagcagaatc aatcccattt ttatatggca
    39781 tatcaatgtc aataatcaat aaatctattt tctcattttt tataaattca attgcctcta
    39841 taccatcctt aactttttta acataatcta catatccaga ctctttagct atatttgcta
    39901 tatcatctct tacaatctta tcatcatcac aaatcaaaac ttttatcata gacttttctc
    39961 ctcctaacta ataatatttt gggattaaga ttacaaattc agttaaaaaa tcatcactat
    40021 ttacaagtat actataatca tacttatcta atatattttt aactaaatgt agcccatatc
    40081 ccctatcttg tccttcttta gtactaaatc cattccaaaa tatactttca acattatgta
    40141 ttttaggacc attatttttt atagctataa tatactgaaa ttcatcttca tatgcatgta
    40201 atcttatata cttcttttct ttttttatag tagacaaaga ctctattgca ttatctatta
    40261 tgtttaatag tactgaactc atatcattag gttcaatctc taaattatcc ataaaatctt
    40321 gaatgtagac atcaaaagat atatcctttt caagacattc ctcgtatttt aatgaagcta
    40381 tagcatctaa ataagatact tttccatacc ttgaattttt tgatttaaag ctatttgata
    40441 tattcattat atattctttg gcatcttcat ctagcccagt atttaataga gaatacacta
    40501 cttgcaaatg tttcaaatag tcatgttttt gacttcttaa tttgataatt atattatcct
    40561 tatgatttat tttactatct agtttttgta atttatattc ctgttcatta ttattattta
    40621 tcaatcttat aactttaaag cctgtataaa aacttaaaat aattaaaacc aaaaaaagta
    40681 taatatttaa tactccttca agtaaaaaac tatatcctaa ccttaattgt atatatttag
    40741 tgccctccat aaataaaatc aatgcaaaac acgatataaa aaatgttaat atgcctcttt
    40801 ttaatatgtc taattctttg taaaatttat acttattcat tataagattg ctaaatataa
    40861 acaaagttag tattactata ggaaatacaa aattgttatc catgattccc ctcccctcta
    40921 caatttatat agggattata tcattttaaa aaaattatgt aaaaagttaa aatttacata
    40981 tatattaatt aaacttgtaa aaatacagat gaatttgtac aattaactac ttaaaatttg
    41041 aaaatactta ctttgctata tattttagat aaatatataa ttgatttagt taagaaagga
    41101 agtggtaaat atgaaagata atttgaaaat agtactaagg tatataaaaa gttataaagc
    41161 taggtcttta gcaattgttg tgagtataat tcttgctaca tcactcgttg ttggtattgg
    41221 tactctctca agaagtgctc agcaagcaga tgtagataga ttgaaaagag agcttggaac
    41281 tgaccatgta tactttaaaa atataaagaa aaatcaatta aagtccataa aatctagcaa
    41341 agatattaaa aatctgggta taacatctta ctatgcgtct acagatgtta atgaaaaatt
    41401 acctataaac atactatata caaataaaaa ttatttgact agtcattcta aacttataaa
    41461 aggtcgattt ccaaaagcta gcaatgaggt ggtagtcgag aaatggattt taaatagtat
    41521 gggcttaaaa ccagagataa atcaaaacct tacttttaaa ttatatcaaa aagaaaatcc
    41581 agaaactttt aaagttgttg gaatccttga agatagattt atagagaaaa aaaagggaca
    41641 atgtgaaatt ttttctaatc tcaatgaatc agaactgaat gattttacaa cttatattga
    41701 atttaatgaa ggcagtgata taaatacaaa catagacaat ataataaaaa atgctatgtt
    41761 agatgagaaa agtataggaa gaaactcaat gcttatagaa tctactatga aaaacggtac
    41821 tttagataat tcaagtaaat acgttgcaat tgctatgagt ctatttgctg gtattgtaat
    41881 atatagtatc tatgttatat caatatacca aagagtacag gaatatggcg tcttaagagc
    41941 tattggttcc accaacttta ggattttcaa acttatgctt tatgaacttt tcatacttgc
    42001 tctaatagca atgcctatag gtatatgtat aggtatggga ggtgcacaaa tatttaatag
    42061 gtctgtagga aatattcaat ttgaaggaaa tataaatgtt acaccttttg ttattccaga
    42121 taaaataatt ctcttatcaa ttgcatgtac aatactaata atactaatta taagtttctt
    42181 tacatactta aaaataagaa gaatttctcc tattgatgct atcagaaaaa cttttggagc
    42241 tgataaaaat gtaaccaaag ttaattattt aatatcaaaa ttaactttaa atatgtcagt
    42301 aacaaaatct atatctgcga aaaacatctt tagaaataaa aaggggttca ttataattgt
    42361 attatctatg agtataggag gtataatcgt cataaaagac aattataaat attctttttt
    42421 agatgtgcaa aacaaatcta ctcaagaaca aacatatatg aatgcagatt ttattctatc
    42481 aaatgtttat ctcaatgaaa ttggtaacaa tacttctttt aaagatataa aagggtttaa
    42541 tgataatcag atagataaaa taaaaaatat agatggaatt gacaaagtca aaactgctag
    42601 tatcttaaac acaagaatag aggtaaataa aataaataga ttagattact acgaagctat
    42661 aaattcaact ccttattata agctaaatcc tctgttcata aaaaataaga ctactggcaa
    42721 atatacacta aagcaaaagc taaaaggtta taatgatgag atgataaagt cattagaaaa
    42781 atacttagta agtggaaata taaacttgaa aaaaatgaaa aaagaaaatt tagctattgt
    42841 atatgtacca gaaatttcta aaacacagaa atacacaaag acttttactc ctggtgaagg
    42901 aacaccagct gtagatataa aagtaggtga tactataaaa gtcaaatatc caaagggtgg
    42961 gatggataca ggtctttatg aaagtctaaa agataattat gaatacttag aatatgaatt
    43021 taaagttgga gctattataa gttatccttt tgcagataat ggtatgtact ctggagatga
    43081 tggtgtagat gtaataacga gtagcgaata cctaaaaaaa ttaactggtg tagataagta
    43141 taacttagtt tatgcagacc ttaatgaaaa tgcaaacaag aaaaaaataa ataacctttt
    43201 aggtaaaatt ggaagtgaag ttcctggaac tacaacagta gatatgatgg tagaaaaaga
    43261 aaattctgat aagatgacat ctagaactat gatttatagt tatggtatag tagcaataat
    43321 gttcataatc agtgtactta acataataaa caacataagt tataacttaa cttcaagaac
    43381 tagtgaattt ggaatgttaa gagctattgg tatctctgag agaggcttta aaaatatgat
    43441 actttatgaa ggaatattat atggtgtact ctcaagtata attactatcg tgtcaggaat
    43501 tataatacaa tttaaaatgt attatatgca gggctttgta tcatatggat tgggattttc
    43561 aatagattac aaaatatata tactagttgt tgtagcaaat ataattgtag gtattctagc
    43621 tacatatatt ccatcaagaa agataaataa aattagtata gttgaagcta taaacattac
    43681 agaataggag gaataattat gcctatattg gaaactatta acttaggtaa gatttatgga
    43741 aaaaaggaaa catctgtaca tgcactaaag aatgcaaatt taaaaataaa taaaggcgaa
    43801 tttgttgcta taattggacc aagtggaagt gggaaaagta catttctaca tttagtaggt
    43861 ggtcttgaaa gaccaagtaa tggtactata aaggtagatg gtaaagatat atgttgtctt
    43921 tcagataaag aattagcaag atatagaaga caaaaggtag gatttgtctt tcaacagtat
    43981 aacttaattc ctgtattaaa tgtaaaggaa aatatagaat taccactaaa gttagacaat
    44041 aaaaaaatag ataaagaata tattgatgac cttataaatt tattgggtct taatgaaagg
    44101 aaaaatcatc ttcccaacca attatctggt ggtcaacagc aaagagttgc tattgcacgt
    44161 gctttatctg caaaaccaag tataatccta gcagatgaac caacaggaaa tttggacagt
    44221 aaaactacag aagaagtcat ggatttacta aaaagttcta taaaaaaata taaccaaaca
    44281 cttataataa taacacataa tgaaaatatt gctagaaaag cagatagaat tatatctata
    44341 atagatggag agcttaaatt aacactctaa aactatttat agaaaagagg atttaaatgt
    44401 tattgatttt aggtaattta aatttaaact tggattttga ctacagaata ataagagaag
    44461 aaaatgaaga tgtagatata tttatagata ttaattatcg tagcttagat atagatactg
    44521 atggttctaa cttatttaat tctagaatac aatttccatt tgtaagagca ttgattctaa
    44581 ggctaaataa gaacaatcaa tgtatgacaa ttcatttgtt aagagatata gatttattct
    44641 ctgcttttgc caactttgaa gtggattata ctgactctat aataaatata aaaaatcaaa
    44701 atgagaaagt aattttaaat aaatctatca aaaaatagta agggggcctt aataggcccc
    44761 aatttttttc ttttctaaac ttattatatc atgataaata aattagtcaa tagaattttc
    44821 tgaatatttt tataattttt tcataaagtt ttttatatat tatgctttaa aattatatgt
    44881 aataaaaaaa ttttccgact aattttaaag ttaattgatt aaatacacat attcgaaatt
    44941 accattaaat tactgacttt ttatcatttc catttagaat ttaaattata atctattatt
    45001 tatagttact tctacgtgtc tctaataaaa ctctatttat attcaaatct aataatttat
    45061 atacaaaccc aatagaacct atgttgcaca atagatatta tttaccataa taaactttta
    45121 gatatatctc ttttaattca ctcatcaatg ggtatctagg attagcacct gtacattggt
    45181 catcaaaagc ttgttcaacc atagcatcca atctttctaa gaatttagat tcttctactc
    45241 cagcctcttt tatagtctta ggaagaccta cttttgcctt tagttcatct attgctttta
    45301 taagattatc tactttttct tggtctgtac ttcctttaat acctgcaaaa gaagcaaatt
    45361 cagcatatct ttgtatgcaa tcaggatatc tgtattgtga gaatgcaccc atcttatttg
    45421 gagcttctgc acaattgaac ttgattactt catttattaa taatgcattt gcaacaccgt
    45481 gttgaacatg gtggaatgca cctaatttat gtgctaaaga gtgacatatt cctaagaagg
    45541 catttgcaaa agacattcct gccatagtag atgccattgc cattttttct cttgcttctt
    45601 tatcattctt tccattttta tatgcacgag gtaaatattc aaatattatt ttacctgctt
    45661 gtagtgctag accgtcagct ggttcagttc ttaacataga tacatatgct tctaatgcat
    45721 gtgttaaagc atcaacacct gatgctgctg ttaatcttgg tggcatctcc atcatcatat
    45781 cagcatcaat tatagccatg tttggcatta attcataatc tgctaatggg tactttacac
    45841 ctgaatcttg gtcagttata actgcaaatg gagttacttc tgaaccagtt cctgctgaag
    45901 ttgggatagc agcaaaatac gctttttcac ccatcttagg aaatacatat actctttttc
    45961 ttatatccat aaatctcata gctaagtctt ggaaatctac ttctggatgc tcatacatta
    46021 cccacattat cttaccagca tccattgcac ttcctccacc tacagctatt attaaatctg
    46081 gttggaaatc agccatagct ttagctcctt ctcttgcaca agctaatgtt gggtctggag
    46141 ctacatcaaa gaatgtagta tgcttaattc ccatctcatc taataaatca gttaatttct
    46201 tagtatatcc attattatat aggaaagaat ctgttactat aaatgctttc tttttatcca
    46261 taacatcctt aaattctctt gctgctacac ctaaacatcc ttttttgaaa taaacctttt
    46321 ctggtgctct gaaccaaagc atattctctc tcctttcagc tactgtcttg atattaagca
    46381 aatgttttac tccaacgttt tcagatactg agtttccacc ccaagaacca caaccaagag
    46441 taagagacgg tgccaattta aagttatata aatcacctat accaccttgt gatgatggag
    46501 tattaactaa tactctacaa gtcttcatag catttacaaa cttttctact ctctctggat
    46561 gatttataga gtctgcatat aatgaagatg tatgaccata gcctccatct gcaactaatt
    46621 tttctgcttt agcaactgca tcttcaaaac ttttagactt gtacattgca agtacaggag
    46681 aaagtttttc atgtgcaaaa ggttcattta attcaacaga ttctacttca ccaattatta
    46741 ttctagctgt ttttggaact tctacatccg ccattttggc tattacatgt gctggttgtc
    46801 caactattct ggcatttaat gaaccatcaa ttattactac ttttcctact ttttcagttt
    46861 cttctttatt taggaaatgt gctccacgat atttaaattc ttttttaact tcttcatata
    46921 ttttttctgg tactattaca gcttgctcgg aagcacatat cataccatta tcgaatgatt
    46981 ttgatactaa gattgaactt acagccattt ttatatctgc actctcatct attattgcag
    47041 gtacatttcc aggcccaact ccgacagctg gtttacctga agagtatgca gatttaacca
    47101 ttccaggtcc accagtagct aatataaggt ctgcctctgc catgacaaca ttagttaatt
    47161 caatcgaagg aacatctacc caacctataa gtccttttgg tgctcctgcc ttaactgctg
    47221 catcatgtac aactctagct gcttctatag tacagttttt agctcttgga tgaggagaga
    47281 aaattatacc atttctagtt tttagtgcta ttagggattt gaatattgca gttgatgtag
    47341 gattagttgt tggtataact gctgataaaa cacctactgg ctcagcaatt cttgtaaaac
    47401 caaatgcttc atctttttct atgacaccac aagttttagt atctttataa gcattataga
    47461 tatattctga ggcaaaatgg ttttttataa ccttatcttc aacaataccc ataccagtct
    47521 cttccactgc catttttgct agaggtattc tttgttgatt agctgcagtt gaagcagcta
    47581 agaaaatctt atctacctgt tcttgcgtaa atgttgcaaa ttctttttgt gccaatctaa
    47641 tttcttctaa tctcattctt aatgtttcaa cactgttgac aatttcagga ctacatactt
    47701 caacatttga tttttctact accttttctt ttttctccat actaaaaacc tccctaaaat
    47761 aattatgcat ctctaatttt tataaatctt aaatgcatca tctatatttt taacttatac
    47821 ttaaaataat tttatagatt tcatctctat accttagatt ttatttattt tactctctta
    47881 ggtcaagaag tttttttctg aatattttgt caatttattt tttaacaaat atattatttt
    47941 ttttactttt actaaaattt tttatctaaa tgagatatat ttcatttgaa taatttactt
    48001 cgactaaata aaatactaaa atgataattt atatctttca ttttttttac ttattaa
//
LOCUS       DAEOSP010000005        46556 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.005, whole genome shotgun sequence.
ACCESSION   DAEOSP010000005 DAEOSP010000000
VERSION     DAEOSP010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46556
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.005"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(121..894)
                     /locus_tag="KRH54_000203"
     CDS             complement(121..894)
                     /locus_tag="KRH54_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBG8577887.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1280)
                     /locus_tag="KRH54_000204"
     CDS             complement(894..1280)
                     /locus_tag="KRH54_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8577888.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="KRH54_000205"
     CDS             complement(1305..1622)
                     /locus_tag="KRH54_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBG8577889.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="KRH54_000206"
     CDS             complement(1634..1894)
                     /locus_tag="KRH54_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBG8577890.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="KRH54_000207"
     CDS             complement(1894..2190)
                     /locus_tag="KRH54_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG8577891.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="KRH54_000208"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="KRH54_000208"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBG8577892.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="KRH54_000209"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="KRH54_000209"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBG8577893.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="KRH54_000210"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="KRH54_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBG8577894.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="KRH54_000211"
     CDS             complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="KRH54_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBG8577895.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8083..9189)
                     /locus_tag="KRH54_000212"
     CDS             complement(8083..9189)
                     /locus_tag="KRH54_000212"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBG8577896.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="KRH54_000213"
     CDS             complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="KRH54_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBG8577897.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11409..11546
                     /gene="rpmH"
                     /locus_tag="KRH54_000214"
     CDS             11409..11546
                     /gene="rpmH"
                     /locus_tag="KRH54_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBG8577898.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11624..11968
                     /gene="rnpA"
                     /locus_tag="KRH54_000215"
     CDS             11624..11968
                     /gene="rnpA"
                     /locus_tag="KRH54_000215"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBG8577899.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            12000..12251
                     /gene="yidD"
                     /locus_tag="KRH54_000216"
     CDS             12000..12251
                     /gene="yidD"
                     /locus_tag="KRH54_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBG8577900.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            12283..12990
                     /locus_tag="KRH54_000217"
     CDS             12283..12990
                     /locus_tag="KRH54_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG8577901.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12959..13588
                     /locus_tag="KRH54_000218"
     CDS             12959..13588
                     /locus_tag="KRH54_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBG8577902.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13700..15079
                     /gene="mnmE"
                     /locus_tag="KRH54_000219"
     CDS             13700..15079
                     /gene="mnmE"
                     /locus_tag="KRH54_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBG8577903.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15177..17069
                     /gene="mnmG"
                     /locus_tag="KRH54_000220"
     CDS             15177..17069
                     /gene="mnmG"
                     /locus_tag="KRH54_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBG8577904.1"
                     /translation="MKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSIA
                     LMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQADK
                     FKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVYL
                     NSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMSL
                     QEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPRY
                     CPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLENC
                     KIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINAV
                     RKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLTQ
                     KGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGISL
                     YEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLEN
                     KKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRRT
                     RGGKGE"
     gene            17062..17781
                     /gene="rsmG"
                     /locus_tag="KRH54_000221"
     CDS             17062..17781
                     /gene="rsmG"
                     /locus_tag="KRH54_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBG8577905.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17944..18729
                     /gene="noc"
                     /locus_tag="KRH54_000222"
     CDS             17944..18729
                     /gene="noc"
                     /locus_tag="KRH54_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBG8577906.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18864..19637
                     /locus_tag="KRH54_000223"
     CDS             18864..19637
                     /locus_tag="KRH54_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBG8577907.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19639..20502
                     /locus_tag="KRH54_000224"
     CDS             19639..20502
                     /locus_tag="KRH54_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBG8577908.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20535..21677
                     /locus_tag="KRH54_000225"
     CDS             20535..21677
                     /locus_tag="KRH54_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG8577909.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21810..22385)
                     /locus_tag="KRH54_000226"
     CDS             complement(21810..22385)
                     /locus_tag="KRH54_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBG8577910.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKLTPKEETKAQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22645..23937
                     /locus_tag="KRH54_000227"
     CDS             22645..23937
                     /locus_tag="KRH54_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG8577911.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24042..24644
                     /gene="yedF"
                     /locus_tag="KRH54_000228"
     CDS             24042..24644
                     /gene="yedF"
                     /locus_tag="KRH54_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBG8577912.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24717..24980
                     /locus_tag="KRH54_000229"
     CDS             24717..24980
                     /locus_tag="KRH54_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBG8577913.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24995..25180
                     /locus_tag="KRH54_000230"
     CDS             24995..25180
                     /locus_tag="KRH54_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBG8577914.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25283..26479
                     /locus_tag="KRH54_000231"
     CDS             25283..26479
                     /locus_tag="KRH54_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG8577915.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26693..26971
                     /locus_tag="KRH54_000232"
     CDS             26693..26971
                     /locus_tag="KRH54_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBG8577916.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27008..27442
                     /gene="ssb"
                     /locus_tag="KRH54_000233"
     CDS             27008..27442
                     /gene="ssb"
                     /locus_tag="KRH54_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG8577917.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27461..27688
                     /locus_tag="KRH54_000234"
     CDS             27461..27688
                     /locus_tag="KRH54_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBG8577918.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27831..28151
                     /locus_tag="KRH54_000235"
     CDS             27831..28151
                     /locus_tag="KRH54_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG8577919.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28159..29115
                     /locus_tag="KRH54_000236"
     CDS             28159..29115
                     /locus_tag="KRH54_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBG8577920.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29126..31120
                     /locus_tag="KRH54_000237"
     CDS             29126..31120
                     /locus_tag="KRH54_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBG8577921.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31121..31570
                     /locus_tag="KRH54_000238"
     CDS             31121..31570
                     /locus_tag="KRH54_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBG8577922.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31589..32917
                     /gene="dnaB"
                     /locus_tag="KRH54_000239"
     CDS             31589..32917
                     /gene="dnaB"
                     /locus_tag="KRH54_000239"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBG8577923.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33122..33430
                     /locus_tag="KRH54_000240"
     CDS             33122..33430
                     /locus_tag="KRH54_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577924.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33512..34801
                     /locus_tag="KRH54_000241"
     CDS             33512..34801
                     /locus_tag="KRH54_000241"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBG8577925.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35127..35202
                     /locus_tag="KRH54_000242"
     tRNA            35127..35202
                     /locus_tag="KRH54_000242"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35160..35162,aa:Lys,seq:ttt)
     gene            35209..35283
                     /locus_tag="KRH54_000243"
     tRNA            35209..35283
                     /locus_tag="KRH54_000243"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35242..35244,aa:Glu,seq:ttc)
     gene            35288..35363
                     /locus_tag="KRH54_000244"
     tRNA            35288..35363
                     /locus_tag="KRH54_000244"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35321..35323,aa:Val,seq:tac)
     gene            35397..35471
                     /locus_tag="KRH54_000245"
     tRNA            35397..35471
                     /locus_tag="KRH54_000245"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35429..35431,aa:Thr,seq:tgt)
     gene            complement(35854..36846)
                     /locus_tag="KRH54_000246"
     CDS             complement(35854..36846)
                     /locus_tag="KRH54_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8577926.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36855..37913)
                     /locus_tag="KRH54_000247"
     CDS             complement(36855..37913)
                     /locus_tag="KRH54_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBG8577927.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38038..39501
                     /locus_tag="KRH54_000248"
     CDS             38038..39501
                     /locus_tag="KRH54_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBG8577928.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNLDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFNVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39603..40400
                     /locus_tag="KRH54_000249"
     CDS             39603..40400
                     /locus_tag="KRH54_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8577929.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40413..42515
                     /locus_tag="KRH54_000250"
     CDS             40413..42515
                     /locus_tag="KRH54_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBG8577930.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            42583..43062
                     /gene="rlmH"
                     /locus_tag="KRH54_000251"
     CDS             42583..43062
                     /gene="rlmH"
                     /locus_tag="KRH54_000251"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBG8577931.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSRMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43129..44820
                     /locus_tag="KRH54_000252"
     CDS             43129..44820
                     /locus_tag="KRH54_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577932.1"
                     /translation="MDRLDIFIENYDFKEYLEKLPRELIEENYKDFVIEFWKNPSVDI
                     IKLKSKYSIKNVVSISSILMQNPIQEYYEFLEDIWKNNIKIDELINKYLITATRMDQI
                     KFPLIYIDEDSFCPYCLGNKFNIINNIKQQDIKVECEKCGEAISYFIHKEEVDNIIEE
                     EKRKKELFIRTLENVYNKINKIKCHRCQSDLYVCINDKIMEYNIRCDRCNSQYESVEI
                     AQEQYRLWQQRAAMMANIKNKENELLLKTLSIKDEKDIKFKLEDIITESESIDVISDL
                     VDMQDRGENIFKQLFNNYVRLGRLDRRLLVTVCKDCKQNENTYTWERGKEHLINMRGI
                     TYSEAVVYELMEKTKLLPIRAIIRRLMEKKLLYCDERSNNLQVHPVLIENLAKLESIN
                     KSQNINNQVAYLIFNKQKYICYHCGESGRPLKIGYLTIDKNINDLSSLVGICDSCYED
                     ITEDEIIIDNIFCVVDERNNELGEAWKFLIQYDSSFRNNTIAEVEHARLYDMYDEDNL
                     IRAYANTLYKQTKDGFANEKVFFAYARAILKNSKGQVSVLQIAKEKFKLEKWLNG"
     gene            45052..46524
                     /locus_tag="KRH54_000253"
     CDS             45052..46524
                     /locus_tag="KRH54_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577933.1"
                     /translation="MSHSKLSDHKLKKGKFIAPFNEFMTPLNSNESWCLGRLPEYIWI
                     GLLINKYGRKKGMEIIYKIIVKLHSIESDIVTPRLSEILSMEDVKQDEFFSYILTIVE
                     KEALSPLTLIFTYSNYPIFVKYFAEPSIKIEDRVTYLDETLRKAMHHQSEFATDIRFI
                     VLYYFCMSGKLVLQKEQINNLLEYPNLEHTDEKMRMLRPFIRAAELMVLNMEKKSDWY
                     LDIFWGRISVMTECKLYATKFEDETANTDLYMEQLHEIFVYLSDLLCKVNPLDEKMLV
                     LLGIAIYSYKRLREVVEHKLFNSISGRGSVRVLIENYIMMKYLVKIEAQHENIWSEYQ
                     FYGIGQYKLIVARYREQEQDLKNSHVDYKYIEALVNEFVIEETIDMDTKYFDKQNIRS
                     KAEIVGEKELYGLYYDYDSAYEHGLWGSIRESSMLKCDNPAHHYHCIPDVDNNQNLKS
                     VWHDCVNIMEKTLLFLDTVYGIPQHLLLEVIKNGKEFFTK"
BASE COUNT        17649 a         5754 c         7453 g        15700 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ccttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgttgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa acacatcatc tttactgtgt
     1141 tttattgcat ttgtgcatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttctta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 atccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tgggttttct ttgacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctattgcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt catcctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatctctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctag cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca acgagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagct cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 gtatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttccta
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaagttt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctaataaaat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccgtctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ctctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcgt gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tgtattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggcaatcctt tctcatatgt
     6181 ctgcttatat atctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcctcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagctcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccctttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatatata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 tacaatactt ttattttagt tccttctatt tcaactatat ctcctgattt tatctttttt
     7801 cctcttctta attctatttc atcatttact gtcactaaac cttcttgaat taaaaatttt
     7861 gcatgacctc ctgttgatgc aatctctgct aatttaagaa attgatccaa ctttatatat
     7921 tctgattcta tagttatttc agtcataata gttcctcctt gtcactattt taaacaaatt
     7981 cctattgata cactaaaagt atattatacc ataaatatgc ttataaaagg agttgtctaa
     8041 gattagaaaa attttctaca aaagacaact ccttcatgtc tgctatatat ttgatgatat
     8101 tcttactgga agcagtaagt aaatataatt aacatcatct gttggtttaa ttatacaagg
     8161 atttacattt gtagtaaatt ctataaatat ttcctcatta tctatatttt tcaaaccttc
     8221 gataaaatat ctagaattga aagctatatc taggtaatct ccatctaaat ctatttcaac
     8281 ttcttcatac acatttcctt tttctgtatt agaagtaatt gccattactt tatctcttat
     8341 agataacttt ataagattat ttttttctga ttgagacaat aaagacgctc tctctatact
     8401 atttaaaagt tccttagtat ttaatttaac tctgctgtta tgttctcttg gcaataattt
     8461 tttataatca ataaaatctc cttcaagcaa tcttgtaata attttcgtat cattaattat
     8521 aaaaatagca tttttttcgt taaatccaac tttaacatta tcctctccag aaagcaaact
     8581 atttacatct ataagtgtct ttccaggaat tatgactttt atattttcag ttaaactatc
     8641 aaccgaacaa ctttttacag ctaatctata tccatcaatt gctactaaat ttaagtttct
     8701 atctacaatt tctaaaagtt ctcccattaa aacaggtttt gtttggtcct gtgagatagc
     8761 aaatacagtt tgtttaatca tattttttag gatttcttga ggtatgctat ataaatcttc
     8821 ttcatttaat tctggtaatt taggaaattc ttttgctgca taacctttaa ttttaaatct
     8881 tgaatttaca caatttatgt aaatattatt ttctgaatca gtttctatct caacaaagga
     8941 atctggtaat tttctaatta tatctccaaa tagccttgca tctacaacaa catcaccttt
     9001 ttctatgatt tctgcttgta cataagtttc tattcctatt tcagcatcat atccagtaag
     9061 ttttaattga ccttcttctg tagatataag tatacctttt aataattcta ttgttgtttt
     9121 tccattaata gctttttgag caattccaat tctatttgct aggatttttt gattacaaat
     9181 tattttcaat gtggataacc tcctctggct ttttatatat aaaaataagt taaaatcata
     9241 gtagtaatag tagtaggtgt tgttgataat gttaataagt atgtataaca ttataaatac
     9301 aaagatatta acatgtgtat aagatgttaa tatctttgtt attttagact tgataatttg
     9361 ttaataattt ttgttcaaaa aaagtattaa caggttattc aagagatata aacatacaat
     9421 tgtgtataaa ttatcccttc aaatctgata taattttgtc tattttagtc ttaatttctt
     9481 cactttcttc tatatcctta gatactttgt catgagcatg aattactgtt gtatggtctc
     9541 tacccccaaa ctcttcacct attttaggaa gggataaatc agtaagctcg cgcgttaaat
     9601 acatagctat ctgtcttgga taagctattg aacgtgttct ttttttagaa ttaaaatctt
     9661 ccatctttaa attgaataca gaagatactt tttcttttat tctgagaaca tttatttctt
     9721 catttttaga agttgttata atatctttta aagcttctgt agcaagatca aaagatatta
     9781 ccctattgct aagagaagaa taagcaacca ctctagttaa tgcaccttct agttccctta
     9841 tgtttgactt aatatttttt gctatatatg acataacctc atttggcaca tctatacgtt
     9901 ctagttgagc cttttttcta agtatagcaa ttcttgtctc aaagtcaggt gcttgaatat
     9961 cagttataag tcccatttca aatctcgatc ttagcctatc ttctagtgta ggtatgtctt
    10021 ttggcggtct atcactagat ataattattt gtttgtttgc ttcatgaaga gtattgaatg
    10081 tatggaaaaa ctcttcttga gttctttctt ttcctgctat aaattgaata tcgtcgatta
    10141 gtaatacatc tacgtttcta tatttattcc taaattcttc gtttttatcg tcttttatag
    10201 agtttataag ttcattagta aatttttctg aagaaacgta aactactttt gagtcttttt
    10261 tttgactaac aatatgatga cctatagcat gcattaggtg agtcttacct aatccaactc
    10321 ctccatacaa aaaaagtggg ttataagctt tagctggaga ttctgcaact gcaacgcatg
    10381 cagcatgtgc aaatctattg ctattaccaa taacgaatgt atcaaatgtg tattttggat
    10441 ttagattggg gtaaagaact ctataattta aatctgtgct atcagaattt aaatcagcaa
    10501 cctctttttc gctcaataca aatttaatat tatatttttt taatgacagt tgactaatag
    10561 catcttcaat taaatgtaaa tatctattct ctaagatatc tttattaaaa tcactaggag
    10621 ccagtaagat taaatcatta agatgtattt ttaatggtac gatgttttta aaaaaggtat
    10681 taaaacttac tgaagttaag tcacctttta ttaattgtag ggttttgtcc cataaagaaa
    10741 ctatatccat aatataaacc tccgaacaga ataattaata ttttccacaa agttattaac
    10801 agaaaagcaa aaatatatat aaattttaca gaataactaa atttatggat aatggtgttg
    10861 ataaattaat aatagtataa attattaaaa aaatatgaaa agatataaac aatatgtaga
    10921 taaagttata aataacataa acatacaata aatggtgatt ataactagag tattgtcagt
    10981 aatcttaaaa gttatacaca aaagtatcca caagctgtga ataatttcta ataagttgat
    11041 aacatttatg cacattatca acaagtatat ttataatatc aaaatatatg tcaaacttca
    11101 atgatttgta actaatttat ccacaatatt aactaattgt tgataaaaat ataaacatgt
    11161 taaaattact ttatttgtgt atataattga aaaaaataat ttagaataaa attaaataga
    11221 tttttgctag tattttcaat aaaaaaaata cataatatat atatggtaga ttttgtgata
    11281 taagcgtccc aaaaactcgt tgacaacagt agtaaaagta tttataatta tatgtgttat
    11341 gtttactttg agggtaaagt tattattttt ataaacggaa aataaaatct gaaaggcggt
    11401 gcaaaataat gagtaaaaga acttatcaac caaaaaagag acaaagaagt aaagaacatg
    11461 gtttcagaaa aagaatgaag acgtctaacg gaagaaacgt gttaaagaga agaagagcta
    11521 aaggaagaaa tagattaact cattaattac aaaagggccg ctgcaaaggt ggcctttttg
    11581 tgtaaataat tacaattcat agctgtgttt atggaggtta actatggact ttaataggac
    11641 taaagggttg aaaaaagact ctgattttag aaaagtatat aaacacggca aatcttttgc
    11701 aaacaaatat ttagtaatat atatacttaa aaataaatca gattatagta gagtaggtat
    11761 ttctgtttca aaaaaagtag gaaaagctat tactaggaat agagtaagaa gattaataaa
    11821 agaagcctac agattaaata ttgatgaaaa aattaagcct ggatatgata tagtatttat
    11881 agcaagggta tctagtaagg atgctacttt taaagacata gacaagtcta taaaaaattt
    11941 agttaaaaga acggatattt ctatttaatt gtatgctata aatatggagg ttaatgactt
    12001 tgaataaaat aatttatata ttaaaggaaa taagtaagta tttatcgaat ttatgtattt
    12061 atttagtgag attttatcaa aagtatatat ctcccttaaa gggacctacc tgtagatttt
    12121 atccaacttg ctcacaatat tcaatagagg cgtttaagaa atatggattt gtaaaaggta
    12181 tgtatttaac gataagaaga attttaaaat gtcatccttt tcatccagga ggttacgacc
    12241 ctctaaaata aaagagagta actattagga ggtataaaaa aattgaatat aataggaaat
    12301 ttcttaggat tgattcttaa ggtaatattt gaatttgtta atcattatgg tatttctata
    12361 atacttttta cgatacttgt aaagattatt ttattaccat taacaattaa gcagactaag
    12421 tcaacaaagg caatgcaaga tatacaacct cgtataaaag agatacaaga aaaatataaa
    12481 aataagccag aaaaacaaaa tgaagaaatt gttaagttat atggagaagc taaaattaat
    12541 ccactatctg gttgtttacc tttattaata cagtttccta tattaatagg attatttagc
    12601 gttttgagag aaccagttgc tcatggcgtt tttgcaaaca aagctgcatt tttagcagct
    12661 gacaatgggt ttttatggat aaaaagctta acatcaccag attatgtatt agcagtattt
    12721 tcaggagcaa gtgcatatgt catgcaaaaa gtaatgacac caaaagatca gttacaaggc
    12781 tctatgaaag taatgacata tgtaatggca ggtatgtctt tctattgggg atttatattc
    12841 ccagcaggac ttacattgta ctggacagta agtaacttat tctcaatagc tcaatattac
    12901 ttaataatga atcctttaaa agctaaactg gctgctaact ctaaggagga aaaagttaat
    12961 gagaaaaagc ctaaaattaa taaaaagtaa gagtaaagag gaagctataa gtaaagctat
    13021 agcagagttg aatttaaaag cagaggatat agaagtagaa atacttgaaa atccaagtaa
    13081 agggttttta ggttttatag gtgctaaaga tggtacttat gaaatttttg ttatcgagag
    13141 ggaagtagat gtagctaaaa attttataga ggtaatgttg aaaaatgcaa atgttgatgc
    13201 caaagtcaat gtatctcaaa aagataattt aatcatggtt gatattgaag gcaaagaagc
    13261 tgcaagttta attggtagaa gaggggaaac tttagattct attcaatttt tgactggatt
    13321 agctcttaat aaaataaata aagattctca tacaagagta cttgtagata tagaaaatta
    13381 tagatcaaaa cgagaagaat ctttaataag atatgctaat aaagttgcta gagaagtagc
    13441 gaaaacaaga aaaactaaaa aattagatta tatgaatcca tatgagagaa gaataataca
    13501 ttcagctctt caaaatgaca aatatgtaat tacatatagt gaaggaaccg atccatatag
    13561 aagattagta attgagtgta aaagataaag tcaacctcct aaacattagg gggttttatt
    13621 ttttaatatt attaaatcat taatgattgg ttaaatgtaa atctatatgt aaaaaattat
    13681 gaattaagag gtgataaatt tgtttataga tgatacaatt gcagcaatag ctacagcacc
    13741 tggagaaggt ggtataggta tacttagaat aagtggagaa aaagctttga aggttgcaga
    13801 agaaatcttt aaatctatgt ctggaaagag tattgaagaa tacaataaga gaacattaat
    13861 atatggaaat atagtggata atgaaaatat aattgatgaa gttttattgg catatatgaa
    13921 aggaccaaat tcctatacag gagaagatgt tatagaaatt aattgccatg gtggatttat
    13981 ttcggtaaag aagatattag aattaatact atcaaaagat gttagattag cagaggctgg
    14041 tgagtttact aaaagagcat tcttaaatgg aagaatagat ttatctcaag cagaggctgt
    14101 aattgatgtt ataaaagcca aaactgatat agctcatgaa gttgctcaaa atcaattaga
    14161 aggttcttta tcaaagaaga taagagaatt gagagataaa gtcactgaga tattagctca
    14221 tgtagaagtt gctatagatt atcctgagga agatattgag catataacat atcaaactct
    14281 taaagaaaaa actgatgaac ttaaaaaaga cattaaaaaa ttatatgata cagctgaaag
    14341 tggaaaaata cttagagaag gcttaaagac ggtaattgtt gggaaaccaa atgtaggtaa
    14401 atcttcacta cttaattcta ttttaggaga gaatagagca atagttactg atataccagg
    14461 aactactaga gatgttatag aagagtttgt aaacataaaa ggtatacctt taaaaattgt
    14521 tgacacagca ggtataagag atactgatga tattgttgaa aaaataggtg ttgaaaagtc
    14581 taaagaatca tttactagtg cagatttgat agtaatggtt ttagatgcat ctagaaagtt
    14641 gtcagaagaa gatatagaga tattagaaaa acttaaggat aaacaaacaa tagtgctgtt
    14701 aaataagaat gatttgaaac aagagattga agaagaaaaa atacttaaat atgtagaaaa
    14761 taatagtata ataaaaatat cagctttaca acaagaagga atagaagaat tgcaagataa
    14821 aatagagtct atggtatata aaggtagtat caaaaataat tctagtttag tagtaacaaa
    14881 ttcaagacat aaagatgcac ttagtaaagc atataaatct gcaacagatg ctttaattgc
    14941 tttggaacaa agtatgccat ttgactttgt agaagtcgat ttaaaaaata tatgggacta
    15001 tcttggatat attaatggtg atacagtaac agaggattta ctagataata tattccataa
    15061 tttctgtatt ggtaagtaat gtacaaaagt aaacttgttt ttattataga gatttttaaa
    15121 aaatttgaag tgtttctcat gaaacattaa ttttgaaaag aaaggaatta taaatgataa
    15181 agtttgaagc aggaaaatat gatgttatag tagtcggagc aggacatgct ggatgtgaag
    15241 ctgctttggc tactgcaaga atgggatata aaactttgat aataacaatg tctttggatt
    15301 ccatagcatt aatgccttgt aatccatcaa ttggagggac tggtaaaggt caattagtta
    15361 aagaaataga tgccttaggc ggtcagatgg gattaaatat agacaagaca tatatacaaa
    15421 gtagaatgtt aaatactgcc aaaggtccag cggtacactc tttaagagca caagcagata
    15481 aatttaaata tcatgaagaa atgaaaaaga cattagaaga tgaaccaaat cttgatatag
    15541 ctatggatga agttgtagaa atacttcatg aaggaaatgt tgtaatagga gttggaacta
    15601 aactaggatg ttcatttaaa tctaaagctg taatattggc cactggagtt tatctgaact
    15661 caaagatata tatgggtgaa gttgcatttt atgaaggtcc taatgcattg gggtatgcaa
    15721 aatatcttac agatagctta gttgagcttg gccttagaat gagaagattt aagacgggaa
    15781 ctccagcaag agttcataga gatagtatag acttctctgt aatgtcttta caagagggtg
    15841 atgaaaaagt aactccattc tcatttatga atgaaaatat agaaaaaaaa caagaacctt
    15901 gttatttaac aagaactaca gaagaaactc aaaaagttat actggataat ttaaaaaggt
    15961 ctgctatgta tagtggagta atagaaagta ctggtccaag atactgtcct tctatagaag
    16021 ataaagtagt aagatttagt gataaaacat cacatcaatt atttatagaa ccagaagggt
    16081 taaatactaa agaaatgtat atacaaggaa tttcaactag tttacctttt gaagttcaat
    16141 tagatatgta caaaactata aagggacttg aaaattgtaa gataatgaga cctgcatatg
    16201 ctattgaata tgattgtgtt gacccaactc aactaaaaat aagtttggaa ataaagggtg
    16261 ttgagaactt atttagtgca gggcaattta atggaacttc tggatacgag gaagcagcag
    16321 ctcaagggct tatggctggt ataaatgcag ttagaaagat agaaggaaaa gagccatttg
    16381 tattagaccg ttcagaagct tatataggtg tcttattaga tgaccttgtt actaaaggaa
    16441 ctaacgagcc ttacagaatg atgacatcaa gagcagaata tcgactatat ttaagacaag
    16501 ataatgcaga tatgagatta actcaaaaag gttatgatat aggattagtt aaaaaagata
    16561 gatatgaaag gttcctaaat aaaaaggctg ctgtagagaa agaatttgaa agactaaaaa
    16621 atgaaagagt tacaccaaaa gaagtaaata gtttgttaga agaaaaagga gcaacaccaa
    16681 taaaggttgg aatatctcta tatgaatttt taaagagacc agaggttact tacgaattgc
    16741 ttgaagaatt agggaaaggc gctggagaag atgtttcaag agaagttaaa gaacagtgtg
    16801 taataataac taaatatgaa ggttatatag agaaacaatt gaaacaaata gaccaattta
    16861 aaaaacttga gaataaaaag ctggatgaga aaataaatta ttcatctata gaaggtctta
    16921 gattagaggc tagacagaaa ctagatgata taaaacctat ttcaataggg caagcatctc
    16981 gtatatctgg tgtttctcca gctgatatat cagttttact tatataccta gaacaaatca
    17041 gaagaactag aggtggcaaa ggtgaataat atagaacttc tgaaaaacgg gattgaaggt
    17101 tttaatatag atacgaatga cagtatgtta gaaaaattta aaatctatag agagatatta
    17161 gttgactgga ataaaaagat gaatcttact ggaatagaag atgaaaaaga agtgtatata
    17221 aaacattttt tagactctat ttcagctgta aagaatggat atataaaaaa tgggatgtct
    17281 ataatagatg ttggtacagg tgctggtttt cctggaatac cattgaaaat atgtttagat
    17341 agcttagaac ttactttgtt agattcgtta aataaaagaa taaacttttt agaggaagtt
    17401 agtagagtac tagagttaga taatattaca tttatacatg gaagagctga agattttggt
    17461 aaaaatgaag actataggga aaaatatgat gttgctacgg caagagctgt tgctggatta
    17521 ccgattctta tggaattttg tgtaccattt gtaaaagttg gagggtattt tatttgctta
    17581 aaaggtccga atgctaattt agagctagaa gaatcacaaa aggctattga tgttttagga
    17641 ttggagtata ttgaaaaaat agatgtagag ttaccagagg tagatttaaa tcataatata
    17701 ttagttttta aaaaagtgat ggaaacacca gttaagtacc ctagaaaagc tggtaaacca
    17761 gccaaaaatc ctattaagta atgaaaaatt tattattggt aagcttaatt atttctcagg
    17821 aaataattaa gcttactgta aaaaaacaaa aataaaggaa ttattaaagt agatgcagaa
    17881 ataaatttgt atgtacatat agagaacaaa aataatttat atctaaaata agagaggtat
    17941 gttatggaag ataaaagagt tatggagata cctatagaag atgtagttcc aaatccatat
    18001 caaccaagaa aaatattttc acaagtttca ttggaggaat tgagcaattc tataaaggta
    18061 tatggtataa ttcaacctat aacagttaga gcaaaagatg gcaaatatga actaattgca
    18121 ggagaaagaa gattaagagc agctaaatta gcagagctta aaaccatacc agcaattata
    18181 aataatatga acgatgaatc ttctgctgtt ctagcgttat tagaaaattt gcaacgagaa
    18241 gacttaaatt tcattgaaga agcaattggt tatgaaaact taataaaaga acatgctttt
    18301 actcaacagc aattagcaga aaaattgggc aaaaatcaat ctactgtagc aaataagctt
    18361 agaatattaa agcttccaaa cgatataaaa atgaagctaa tagagaataa tttaacagaa
    18421 agacatgcta gagcatttct taaactacca agtgaagatt taatgcaaag tgtgttagac
    18481 aaagtcataa aaaatgaatt gactgttaaa aaaacagaaa aattgataca agatgtatta
    18541 gaagaaacta aagttcaaga agagcctgat aaaaaacaga atataaaggg tgctatgagt
    18601 ataagaattt acataaatac tataaagcag gcctttgatg ctatatcaaa tacaggaatt
    18661 gatgctaagt ataatgaaat agataaggga gattatatgg aagttgttgt aaaaatccct
    18721 aaaaaataga gattactgct taccagtaat ctttttttgt aatataagcc ttgtaatata
    18781 aattgattta tagacattat ttatacttaa aaaacaccta ttcaacattt aaaattaatt
    18841 taattcttaa aggggggaac atcatgggca aagttatagc tgtattcaat caaaagggtg
    18901 gagtcgggaa aactacaaca aatgtaaatt taagtgctag cttaggaact ttgggaaaga
    18961 aaatattagt attagattta gacccacaag gaaatacaac tagtggatat ggtattaata
    19021 aaaacgaagt agaaaataca atctatgaaa ttatgttaga tgggttacat attaaagaag
    19081 caataataag tactgaattt gagaatgtag atgtagttcc atcagctact gaactatcag
    19141 gtgcagagat agagcttaca tctaaaacta atagagagta tattttaaaa aattccataa
    19201 aagcggtaat agatgaatat gattatatat ttttagattg tccaccctca ctggggatgc
    19261 ttacaataaa ttgtttaact gctgtagata gtgttttaat tccaatacaa tgtgaatatt
    19321 atgctttaga aggtgttagt caattaatgg agactataaa attagtaaaa tcaagattaa
    19381 atgctgatat agaaattcaa ggtgttgttt taagtatgtt tgatggaaga gctaatttat
    19441 caatacaagt agttgaagaa gttaagaaat actttaaagg tagtgtatat acaactttaa
    19501 ttccaagaaa tgtaagactt gcagaagctc caagtcatgg aaaacctgta atttattatg
    19561 ataaaagatg tagagggtct gtagcttatt tggagctagc agaagaattt atagatttag
    19621 aagaggagga atggtagtat ggagaataag tctaaaagaa gtaatagatt aggtagaggt
    19681 cttagtgcat tgataccaga aattaaagga gaaacttcag aaaaagaaat agtcaacata
    19741 gatatagaca aaatatatcc aaacgaagtt caacctagaa agcaatttga tgaagaaaag
    19801 ataaaagtat tgtcggattc tataaaaaat tatggtgttt tacagccaat agtggttaaa
    19861 atggatgaaa ataataaata tatgataata gctggagaaa gaagatttag agcatcaaaa
    19921 cttgcaaata aaaatcaaat acctgctata attaaagata tagatatgaa agatataatg
    19981 gagatagcct taattgaaaa cttacagagg gaagatttaa attcaataga agaggctcta
    20041 gcatataaga gtttaataga gcattacaat gtaactcagg aagaaatatc agaagctgtt
    20101 gggaagagta gacctcatat aactaataca ctaagacttt taaatcttgg acaagatgtt
    20161 atagaaatga tagacagtgg tagaatcaca gcaggccatg gtaaggcttt attgagaatt
    20221 gcagataaag atttgcaact tcaaatagcg aaaaaaatag aagaagaaga actttctgta
    20281 agagaagtcg aaaatatagc taaaaaaata tctgaaaata aacaagaaga acctaaaaaa
    20341 tctaaaccta aggatgtatt tattttagat gttgaagata aacttagaaa tatatttgga
    20401 acaaaggtga atatttctaa aggaaagaaa aaaggtaaaa tagagataga atactataat
    20461 gatgatgatt taaatagtat tgtatcaatg cttcttgaat agaacaaaaa tataaaacag
    20521 agaggaagat tgttatgatt tatttagata atgctgcaac tacatatcca aaaccagaaa
    20581 gagtgtataa tgctgtttta gattgtatga aaaattattg tgcaaatcca ggtagagcag
    20641 gccataaact tgctatgaga gctgctaggg agatatatga tacaagagag aatatagcta
    20701 agttgttcaa tgtaagtaat cctatgaata tagtatttac atctaatgca acagattctt
    20761 taaatttagc gataaaaggt gtattacaag aaggagacca tgtaataaca acgtctatgg
    20821 agcataattc agtaataaga cctataaaag cattggaaaa aaggggtatt gaaaatactg
    20881 tagtaaaatg cgattatgag ggatttttag attatgaaga tttagaaaaa tcaataaaga
    20941 gtaatactaa actgattgta acaacacatg cgtctaatgt atgtggaact ttaatagata
    21001 taaaaaaagt tggagaaata gctaaaaaac ataatatact atttttagta gatgcatcac
    21061 aaacagcagg tgtttatgat atagacgtaa atgaatgtaa tatagatatg ttggcaatgc
    21121 caggtcataa atgcttattt ggacctcaag gaacaggtat attatatgta agagaagggt
    21181 tgaacttaaa tatattaaaa gaaggtggaa caggtagtaa atcagaagaa atagttcaac
    21241 cagagttatt cccagataaa tatgaatcag gaactcataa tactccaggt atagcaggtc
    21301 ttaaccaagg tatactattt atatttgaaa gaggaatcaa caatataaga caacatgaag
    21361 aagaattatg tcaatatatg atagacaaat tagaggaagt accagatata aagatatatg
    21421 gaccaaagga cagtaaaaaa agagcatcag taatagctct aaatatagga gatatggatt
    21481 ctggagaggt tactttctta ttagatagtg attataatat agcgactaga tcaggaatac
    21541 attgttcacc actagcacat accactttag gtactttaaa acaaggtgca gtaaggttca
    21601 gtataggata tttcaacaca aaagatgaga tagataaagc agtggaagct ttgaaaaaaa
    21661 tttcaaaaaa taaatagcat gagatttaat aatatagaaa taaaaaaggg gatatttcaa
    21721 aataaaaatt tgaatcatct ccttttttgt acaaaaaaga aaaggggtcc ctaagaacct
    21781 ctaatctatt tgataattgt tttaagaagt tactttaagt aatctccttc ttcaagaact
    21841 atatgaccac tttcgtaatt agaaccatca acagtaattt ttatttgttc aacattcata
    21901 ttttttatgt aagtacgagc aactgaatca agcattaaag tttctgcatc actacctaag
    21961 tttgactttg taaagtcaga gctaacatct aatataccag tttttacacc atcaatgtct
    22021 tttgtagtaa atgaatttaa ttttgcagat tctggaacaa ctttaagttt ttgaagttct
    22081 tcaaataaag tgttctcatc aattttattt aaatcaactt tattttcaat taaatctgtc
    22141 ttatctacat caaaagagta aatagtagct gattgagctt tagtttcttc ttttggagta
    22201 agtttagctt tgttattagg aacctctttt tcagtattgt tttcttcttt tggttgctca
    22261 acatttgtat tgctcttagt ttctttttta gatgaatcac ttccattttg agcattagta
    22321 caaccaactg cgaatataga tatagataat atacttaaca ctaacattat tttcttattt
    22381 ttcataaaaa tttctcctct cttaaaaact attcttatat ataattatag tcgttatgag
    22441 caaatatatg aatacaaaat agtaacaaat ttaaaaaatg atttatttaa attaataaaa
    22501 ctcaaaataa actaaatttt agtcattttt atgggaaatc atttaatgta cagtgaaaat
    22561 attgtaaaat aaaagtagtg tttttaaaat aaatataagt tataattata ttataaaaaa
    22621 atgtcttcaa aaggtgggaa tagtatggaa attttgagct taggagaaaa gattaaaaag
    22681 ttaagaaaag aaaaaaacat gacattgaaa gaattagctg gagataggat aacagcagca
    22741 caaataagcc atatagagag agataaatct catactagtt atgaactgtt ggaatatcta
    22801 ggcgataagc tagatgtaag tgtagaatac ttattagaga ccaaggaaat gcaatcaaaa
    22861 aagataactg ataatttaat actacagagc gaaatttata taaaagaaaa tgaattaaac
    22921 aaggctgagg accaaataaa agaagtttta gatatatgta agagatacga tttaatggag
    22981 aactatggca gatgtaattt tttaatggct aatattaatt tgaaaaaatc taattataat
    23041 gatgctatag aaaactttga aaaggcattg tacttcttta taaaaaatag tgatagtgaa
    23101 aatatactta agtgttattt aaacataggg aaaatatata tgcaggaaga cttttataaa
    23161 ggtgctataa gccactttaa ttttgcagag gaaattttgt cagagaataa atttgaggat
    23221 gttaacatac acaaggaact atatagtaag atggcatatt gttacataaa actagataat
    23281 ccaaatatgt cattgaagta catagaaaag ataaatgaaa tagataatag aaatgataga
    23341 aaagaagatg tcgatatttt ggtgttaaaa gcaaataata tgcttgagat tggaaaatat
    23401 gaagaatcaa gagattactt taaaaaagca ctaaaaatac ttgagagtga ggataataaa
    23461 actggattag caaatgtgta tttaacaatt tgtgatgttt acagaaaaat aggtgaaaca
    23521 gatagagtac ttgaatattc acaaaaggta tatgacataa agaaaaatga tgaagatgaa
    23581 tacatgatgt caggattatt taagataata gaagcatata tagataaaga agattatgat
    23641 ttagctagaa aatattgtaa gatagcctta gcttcatcaa taaaaaataa aaataaattc
    23701 aatgaatata aagcattgag attctatgct aatatgtata aaaatcagaa tgaagtaact
    23761 cttgcaattg aatatctgat taaatgcata aaaatagtat cagatttagg caacaataga
    23821 atattggcca atttatatat agacttaggt caattatatt caaatatatc aaaagaaaaa
    23881 gagttagaat attatcaaaa aggtgtattt atgtacaaaa acttagaaat aatgtagggt
    23941 aaataaacct ataagggtat agaaacaatc tataacaagc aagataatat ctatgataaa
    24001 atataaattg tatgaatatt tttaaggaga tgaatcagac aatgagtata aaaatagatg
    24061 cgagagggct tgcttgtcct aaaccagtta taaatacaaa aagagaattg gataacctag
    24121 aagaaggtgt tgtagttaca atagttgata atgaaacagc taaggagaat atattaaaac
    24181 ttgctaaatc tttaagttgt gaagcaaata ttgttgacga aaaagagaat tttatatcta
    24241 tagaaattag aaaaggtaaa aatatactag ataaacaaaa tgttgtagat aataaagaaa
    24301 gtgaattaga caatacatgt atatttatat cttcagataa gatgggactt ggaaatgacg
    24361 aattaggaaa agtgttaata aaaggattta tatatacatt aacagaatct aaaccttatc
    24421 ctaagtatgt acttttagtt aatggaggag taaaattaag tgctgaaaat gaagaaacca
    24481 tagaaaatct aaaaatactt gagaatatgg gtgtagaagt tttatcttgt ggtacttgtt
    24541 tagattatta taatttaaaa gacaaattac aagtaggtac agttacaaat atgtatacta
    24601 tagttgagac attaaagaat gcatcaaata ccatttctat ataatgtatg tatgtttaat
    24661 aatataaaac taacaataaa gattaactca aatagatagt cagaaaggat taagtaatga
    24721 atcaaatgta tatagtgtca tttaattcga cacatcatgc gataagatca gaaaagttat
    24781 ttggtgaaaa tagtctaaaa gtaatggctc ttccaacacc aagagaaata acagcaagtt
    24841 gtggaatatc aattaaattt tcttttgaag atatggaaaa gataaaaact attttggttg
    24901 aaaataatgt agatataaaa ggaatttatt gtataagtaa attagaaaat ggttcaaaag
    24961 aagtagagaa gttagattag gaggctaatt atttatgcca atggatttaa aaataggtga
    25021 tattgttgaa ttaaaaaagc aacatgcatg tgggtgtaaa gagtttgaaa tcataagaac
    25081 gggaatggat ataaagataa aatgtactaa atgttcaagg ctaataatgc ttgatagaga
    25141 gaccttagaa aagagagtaa aaaagctaat aaaaaagtga taaaaattta acacttaaaa
    25201 aatgtcaaat accgtatatt aatatccaaa attttggtat aataagaatt gaagaaaaca
    25261 ttttcatggg ggtgctttaa ttatggcagt taaatatgca aaaagaatgc aaggattaca
    25321 aggatcagaa atacgtgagc ttttaaaact tactcaacaa ccacaaataa tatcttttgc
    25381 tggtggaatg ccggctccag agttatttcc agttgaggaa atgaaaaaag tatcagttgc
    25441 agtgcttgaa gaaaatggaa gatcagctat gcaatatact acaacagaag gatatgaacc
    25501 attaagagaa aaaatagcag ctagaatgaa tgataaaaac aaaactaatg taaacaaaga
    25561 tgatatatta gttacaagtg gttctcaaca aggtctagat tttgcaggaa aagtattcat
    25621 tgatgagggt gatgtaattt tatgtgaaag tccatcttac ataggagcta taaacgcatt
    25681 taaatcttat caacctaaat tcatagatgt tccaacagat tctgatggaa tgataatgga
    25741 agaattagaa aagatactag aaacaactga tagaataaaa atgatttacg taatacctga
    25801 cttccaaaat ccaacaggaa gaacttggcc acttgaaaga cgtaaaaaat tcatggaaat
    25861 agtaaataaa tttgaaatac cagtaataga agataatcca tatggagatt taagatttga
    25921 aggagagact ttaccatctt taaaatcaat ggatacaaaa ggattagtaa tattcttagg
    25981 aactttctct aaaatattct gcccaggata cagattagga tggacttgtg cttctccaga
    26041 aattttatct aagtttaact ttgcaaaaca aggtgcagac ttacaagcat caactatatc
    26101 tcaaatggaa gtaagcaaat ttatggatat gtatgaccta gatgcacatg ttgacaaaat
    26161 aaaagcagtt tatgttaaac gtagagatgt aatgcttaag actatggaag aagagttccc
    26221 agaaggatta gtatttactc atccagaagg tggattattt acatgggtag agcttccaag
    26281 taatttaaat gctaaagaat taatgccaaa atgtttagat aagaatgttg cttatgttgc
    26341 tggaggagga ttcttcccta atggtggtag agaaaatact ttcagactta actattcaaa
    26401 tatgccagaa gaaaaaataa tagaaggtat aaaaaatata gcagctgtat taaaagaagc
    26461 tatgggtgta gaagcttaat taaaaataat aacttatata taatacaagg gatggttgtt
    26521 gccatccctt gtttgtttga aaagtatata attcacctta tactaaactt tgtccaaaat
    26581 ttaaattgac agaaataaga ataaatgata aaatgtgtag gtatgagatt ttagtctcat
    26641 ttctctgctc taagagtgta gagccgttaa gtccaaaggg aggtgaatta tagtgagaaa
    26701 ttatgaatta gtttatgtgg taaagccaaa ctctgatgaa gaagtaagag aggctatact
    26761 aaacaaagtt aaggaagttg ttgcaactga cggagaaata gttaaagttg atacttgggg
    26821 aactaaaaaa ttagcttatc caatagctaa gtttacagaa ggtttctatg tattagttaa
    26881 cttcaaatca gcagtagacg ttcctaaaga gatagacaga aacttaaaga taaatgaaaa
    26941 cgtaataaga cacatgatag ttgttgctta ataactggtt ttattataaa aaataggtgg
    27001 tgtttttatg aatcaagttg tattagtggg aaggttgact agagaccctg aattaagata
    27061 catcccaggt acaggaacac cagtggcatc ctttacaata gccattgata gagactatgt
    27121 aaaaaaagat ggatctaaag aaactgattt tatacctgtt gaagtaatgg gtaaatctgc
    27181 tgaattttgt gctaactaca taactaaagg tagattagta gcacttcaag gatctataag
    27241 agtagacaat tatcagacac aatcaggtga aaagagaact tttacaaaag taagtactag
    27301 atcagtacaa gctttggata gtaagaataa atcggaaaat tcatatagag agagtgctcc
    27361 agcttttgag ccaagctttg aacctcaagg tttagaccca caaggtttcc aagctataga
    27421 tgatgacgat ataccatttt aaaaaggagg gaaaaaagtc atgatgaata aaaaaagacg
    27481 taagaaaaaa agagtttgtc aattttgtgc tgacaaaaac gctaaaatag attacaaaag
    27541 tactcaaaga ttacaaaagt atataactga aagaggaaag atattaccaa gaagaatatc
    27601 tggaacttgt gctaagcatc aaagagaatt aacagttgct ataaaaagag cgagaaatat
    27661 agcactttta ccatatacat tagactaaaa aaatgagcct tgtgctcatt ttttattata
    27721 tgtatttata tatctaaatg aggcgagcta taaagctcgt ttttttgttt atatacttaa
    27781 atatattatt atttatgata taatattata tattaaggag ggttgtaaac gtgaaaatag
    27841 ataaaggttc tgagataacg agaaacataa agataataga atggatgaaa acagaaatat
    27901 taatgagtgt aagtgatttg tttaatttat tgtttaaagg ggtaaaacca ttagatgaag
    27961 caattcaaga tactttagca aatataataa tgataacata tctcttagca aagagattag
    28021 gaattagttt tagagatgtg gattataaag tcaaggaaaa aataaaaatt ggtatagatg
    28081 aggaccatag cgttgaaaga tggtatggag atttatctaa tttaaaaaaa catatggata
    28141 atagggagtg atattaaatt gaataataaa attagactat ctaaggcaat gtctataata
    28201 gttttagcta taataatcgc tttagtaata gcctatattc caatgctagg aatatttagt
    28261 atgttagcag ctgttccata tgtaattata ggtgctatta ctgatagaaa gtattctctt
    28321 atatctattt tggttacatt ttgtgtactg attctatttg cagacatttc ttatgcatta
    28381 aatatatgta taatgtattc tttaccaggt atagtaatag gaaagatgct aaagagaagc
    28441 tttgaacaag aggacagtaa taaattcgaa ccgatatatg gtggaaccat aatatttgta
    28501 ctttcaatgc ttgtatattt ttttgtatta aagacatttt taaatgtgaa tctattagat
    28561 gaagtttcta agatgatatc agaaattgta aatattcaaa aaaatagttt ttctgcaact
    28621 gaacttaaag tgtttgataa aatgaagcca gatgagattg taagttattt tacaaatatg
    28681 ctaccaatga tgttattttt acaagggttg ttatcagcgt ttgtcacata ttatttgagt
    28741 gtatttttta taaaaaggat tacaaagatg aatataagat ttcctaagtt tgctgatttt
    28801 tatttaccgg gaaatgctat actcacaact ttcttacttt atttattagt cttatttatt
    28861 caaattattg gttcaaaatt atatacagaa ttaataatga ctaatttaca gctagttttt
    28921 aatttaatgt ttgtaattca aggtatagca gtttgcattt atttcttaaa gaaatggata
    28981 agacagggac caaataaaat tatgtttttt tcagggataa ttttatgttt atttggattt
    29041 atgggaatat cttttgttgg tatggtagat agtataattg actttagaaa ggtaagaagc
    29101 tataaatcca cttaggagga tagaaatgag taataaacaa acctttaaat taaatatgcc
    29161 agaaataaat ttatatataa ttgttattgg tatttctagt ataattttgc tttattacaa
    29221 cttgtatgta gggtgtttat ttttctgtat ttttgtatat atggtttttc acaattggag
    29281 aactactaat attagacgtc atgaatggac agagtatatt caaaatttat ctttagatat
    29341 agatgaaaca actaaaaaag ccataataaa tcttccaata ccattatgta ttttagaatt
    29401 tgatggaaat atatcctggt ataatggaaa gttttatgat atgattggtc aaaaagattt
    29461 acttgataaa aatatagagg atatagttaa aaatcttaat ttaagaaaag tattaaatga
    29521 aaataaagag atgtacacag aaataaacta taaagaaaaa gaatatacaa taatttataa
    29581 tgttataaaa aatgatcaag aaaaaaatcc taaatatttg atgatattat actggataga
    29641 caagactgaa tatttaaaag taaaacaaaa ttatgatgat gaaaaaaatg caatgatgct
    29701 aatacaagta gatggatatg atgaagtatt aaaaagtgca gcagaagata agagagcttt
    29761 aattaatgtt gaagtagaga aaatactttc tgctttagaa ttaaattcaa atggtgcatt
    29821 gagaagaact tcaaaagaca aattcttctt ggtaatgcat aaaaaagagt taaaaaaatt
    29881 ggaagctgag aagttttcta ttttagatac aataagacat attgattatg gaaataatct
    29941 tcctgttact ataagtatag gaattggtat agatggagat acattaaatg agaacctaaa
    30001 acttgctacg ggtgctctcg atttagcact tggtagaggt ggtgaccagg ctgttgtaaa
    30061 aacaaaagat aaatttgtgt tttatggagg aaaatctaaa gctgtagaaa agaaaaccaa
    30121 agtaaaatcc agattaatag gtcatgcttt aagagaggtt atacaacaaa gtgaccaagt
    30181 ttatataatg ggacataaat atcctgatat ggatgctatg ggagcagcag ttggtgttta
    30241 tgatatatgt aaatcctgta acaaaactgc aaacatagtt ttacaatctg taaatgaatc
    30301 tatagaaata tttataaata aaataaatga aaataattac tataaaaagt tgtttattgg
    30361 aaaagaggaa gctattgaca actgcactaa aaatacatta gttgtagtag ttgatactca
    30421 tagaccaaat tatactgaat gtgaagaact gttaaagcta tcagagaagg tagtagttat
    30481 agaccatcat agaagaggtg tagagtttat aaatgatgca gtgcttttat tccatgagat
    30541 atatgtatcc tcaacgtgtg aaatggttac agagttagtt cagtatatgg atgaagatgt
    30601 aacaataaat aaacttacag ctgaagggct tttagcaggt ataagtctag atactaaaaa
    30661 ctttgcattc aaaacaggtg ttagaacttt tgaagctgct tcttacctta gaaaagttgg
    30721 agcagatact atagaggtaa agaaattctt caattctgat gtaaaagatt tcataataaa
    30781 agctgaaata attcaaagta ctaaaataat taataataga atatgtttag catattcaag
    30841 tactgagata gatagtataa atgttataat agctcaaact gctgatgagt tattgaatat
    30901 caaagaagta gaagcttcct ttgtattagg tgaaaaagat gatactatat ttataagtgc
    30961 gaggtcttta ggacaaataa atgtacatgt acttatggaa aaattaggag gcgggggaca
    31021 catagatata gctggagcac agttaaaaaa tgtttctcta aaagaagctt ataagatggt
    31081 aaataaaata atagaggagt atttggagga ggaagaatag atgaaagtta tattattaaa
    31141 agacgtaaaa ggaactggta aaaagggaga aatgaaagaa gtaagtgatg gatatgcaag
    31201 aaatttctta tttcctaaaa agatggctgt gcaagcagat agtgtagcaa taaaagaatt
    31261 aaatgaaaag aataaatcta aagaaataaa agcacaaaaa gagtatgaag aagcagtttt
    31321 attaggtaag caaatggaag aaattaatat agaaatatat tcaaaagcag gagaaggtgg
    31381 aagattattt ggttcaataa cttctaaaga aattgctgag caattaaaga aacaaaaaga
    31441 catagatgta gataaaagaa aaatattatt agatgaaccg ataagatctt taggttcaac
    31501 tttcgtagaa ataaaaatac atcaaaaagt aactactaaa ataagagtgg atgtaaaaga
    31561 aaaacagtaa taactagagg tgattaagat ggaagatatg acgagaattc ctcctcatag
    31621 tgtagaatca gaacaatcaa tattgggttc tatacttcta gataaagatg ctataataac
    31681 ggttacagaa actataaagc ctgatgattt ctataaagag gctcacaaaa taatatatga
    31741 atgtatgata actttaagta ataaaggtga gcctatagac ttgataacat taacagaaga
    31801 actaagaaaa caagggcatc ttaatgatat tggaggaatc agttatatta caagtctttc
    31861 tactatagta ccgacgactt caaatgtaaa atactatgct gatatagtaa aagaaaagtc
    31921 tgtacttaga aagcttataa aagcttctaa tgaaataata aatctaggat atagtggagc
    31981 aactaagatt gaagatgttt tagaacaagc tgaaaagagt atatttgata tatcacagga
    32041 gaaaactagt gatgatttta aatctataaa cttagttttg atggatgcct atgatatgat
    32101 agaaaaatta tacactaaca agagtgatgt cactggtata actactggtt ttaaagattt
    32161 aaacaaaaaa ataaatggtc ttcaaaggac tgatttaata ttaattgcag ctagaccagc
    32221 aatgggtaaa actgcctttt ctttaaactt ggttcaaaat gcagccttaa aaggtgatgc
    32281 ctctgtagct gtatttagtt tagagatgtc taaggaacaa ttagtacaac gtatgttatc
    32341 ttcacaatct agtgtagaat taaaaaaaat aaagactgga acacttaatg ataatgattg
    32401 gcctagaatt atagatgcta tggcagtatt atcagatgcc aaaatacaca tagatgatac
    32461 tcctggtata aaaatatcag aactaaggtc aaagtgtaga aagttaaaaa tagaaaaagg
    32521 actagattta gtgcttattg attatcttca acttatggaa ggtgaaggta ataatgaaag
    32581 cagacaacaa gagatatcta agatatcaag gtcactaaaa atattagcaa aagaattaaa
    32641 ctgtcctgta gtagcactat ctcagttatc acgtgcacca gaacaaagag cagaccatag
    32701 acctatgtta tctgatttaa gagagtctgg agcaatagaa caggatgcag atatagttat
    32761 gttcttatac agggacgaat attatcatgc tgattctgag agtaaaaata taggagaagt
    32821 aataatagca aaaaatagac acggtgaaac tggttctgtg gaacttgttt ggcttggtga
    32881 agttcaaaga tttggggata agttgagaga cctataatct aacgaatatc cacagaacgt
    32941 cctttctttc tacaggaaaa atgtggatac atgtcactaa atatgtccaa acattgaaat
    33001 ttagctaaaa aactatccac actatccaca gatggcctgt tgataagtgt gaataagtag
    33061 ataaagcaag cttttggctt gctttatctg tgtataaata ttaattatta ggaggaataa
    33121 tatggaaaaa ataagcttaa aatatatata tcccaatata attaaagtac ttgatgaaat
    33181 aaacttattt agagtaattg acaataattt aagagagtct attgttgttt atgctaataa
    33241 tgtggataat cagtatcata taaacatgac taatacaaac tttggaaaca taattaacat
    33301 atgtaaatta gaaaaacttt tagatgtgga taaatttatg gaaaaagtta taaaatatga
    33361 aaaagaaata atagagaaag aagagttttc aaaaatagaa gaatatatgt taaatattgg
    33421 agaatactaa aaaagttgaa aaaaatacga atgtttgata gaataagtaa gtaaagtgtt
    33481 cgagagtata taaaatatag aggtgaatta catgaaaaca gttgcaattg ttggttctca
    33541 atggggagat gaaggtaaag gtaaagttat agattatctt gctactcaag cagatgtagt
    33601 agtaagaggg caaggtggaa ataatgcagg acatacatta gttgtagaag gtaaaaaata
    33661 cgcattacat ctaattccat ctggagtttt aaatccaaat acagtgaata taatcggaaa
    33721 tggaatagtg tttgacccta aaggattttt agaagaattg gaaatgttta aaacggataa
    33781 tataagtaca gaaaatataa aaataagtga tagagcacat gtcatattcc catatcataa
    33841 agaactagat gcattgtcag aagaagccag aggcgattta aagataggta caacaaaaaa
    33901 aggtattgga ccttgttata tggataaaac agagagatct ggtattagaa tatgtgactt
    33961 aatggataaa gataaatttg ctataaaatt aaaagctcaa atagatgcta aaaatgaaat
    34021 agttaaaaat atatatggaa aagaagaatt atttgatttt gaaactatat ataacgaata
    34081 tctaggatat gcagaacaaa taagaaaata tgtggcagat acgtctgtta ttgtatatga
    34141 tgcagtaaga gcaggtaaaa aagtattgtt tgaaggagca caagggactt tattagattt
    34201 agatttagga acttatccat ttgttacatc ttctcatcca acatctggtg gatttgcaat
    34261 tggtgcaggt ataggaccaa atatgataaa agacgttgtt ggtatagtaa aagcatatac
    34321 tacaagagtt ggagaaggac cgtttgttac agagcaaata aatgaaactg gagataagat
    34381 aagagaacaa ggacatgaat ttggggtaac tactggtaga ccaagaagat gtgggtggtt
    34441 tgatgcagtt atagttaagt atgcagctag agttaatgga ttgactagta tttcatttat
    34501 gttacttgat gtattaactg gatttgataa gataaaagtt tgtacgtctt ataaaatggg
    34561 cgataagata ataacggatt tcccagcttc attagatgat ttagcaaaat gtgaacctgt
    34621 atatgaagaa ttagatggat ggaatgaaga tataactcaa atagataact ttgatgacct
    34681 tccagaaaat gcaaagaaat atgtagcaaa aatagaagaa ctagttggag taagtgtaga
    34741 tatggtttct gttggtccaa atagagctca aactataata agaagaaata tatttgctta
    34801 atattttatc aattaaataa tttaaaatag ttgaagaaga gtatctcaag ataatgttta
    34861 tagttattag agatgctctt ttatatttta cttacattga ttttttatta agttaaagct
    34921 acttatacat attctagctg aaattaaagt tgaatttatg tagatacata taggtttttt
    34981 aaaaagaatt ttgttgaaaa taaatggcta aagatgattt taattatatt ctaaaaaaag
    35041 taaaaaaaac tattgactaa aaattatata gacgctataa tataacttgt gagtgaaaga
    35101 aacgacgaaa aaagtcgaaa aaatgtggtc tattagctca gtcggtagag cacctgactt
    35161 ttaatcaggg tgtcccgcgt tcgagtcgcg gatagatcac caaaatatgg ctcgttggtc
    35221 aagaggttaa gacaccgccc tttcacggcg gtaacagggg ttcaattccc ctacgagtca
    35281 ccagtatggg actatagctc agctgggaga gcacctgcct tacaagcagg gggtcacagg
    35341 ttcgagccct gttagtccca ccaaattttg aattagagat aaatactaga ttatatgctg
    35401 gtgtggctca acggtagagc agctgacttg taatcagcag gttgtaggtt cgattcctat
    35461 caccagctcc acaaagtaca taaattcaaa ttaatataat tttctatgtt acaggatact
    35521 tttaagtatc tttttttaat ttcattttta ttgtattaaa aatgaaatta aacatacaac
    35581 aaaattttga attacttgca atgtgatagt tttaaatata aaatttagaa tcattctagc
    35641 atagtattat gtaatatagc aaatttaatt tatagtatca ataaatatag actactaaga
    35701 atgtaaatga tttgttagat taagatacat tagatatgta aatagaaaaa taaaaagtat
    35761 gttttaattt atgtgatata agttttaaac agagaagcaa gtttgctctt acctctctat
    35821 ttaaaaataa aaaaatgttt tttaagggct attttattta aatctttccc atctcaaatc
    35881 atctccagta aattctaaaa tcctaaactt atctaatatt ctagagagag ttctttgagt
    35941 gtaagtattt cctatttgag atggtgttaa gttggttgaa ataatgattt ttttaccagc
    36001 aacaagtctt gtatttacaa tattgaagat ttctccacta gtaaaagaat tattaagttc
    36061 agttccaagg tcatctatta ttaataaatc acaatcaaac aagtttttat aattatcttc
    36121 acttatttga ttatttgtgt ctcttctaaa tttataatct tcaagaatat ctaaaattct
    36181 aaaagaagtt tgatagatta caacattgcc tttatctaac aattcctttg caatacaatt
    36241 gcacatatag gtctttccta gtccagtaga accataaaac agtagatttt cactattatc
    36301 cttcttaaaa tcatgaacaa aattttcgca gatacttaaa ttatttagca tattttctct
    36361 aggagatatt tcaccatcag agcctttctt aggagaaaat atattcaagt taaaattcga
    36421 aaaattttcc tgacttaaaa tcctatctaa attagacatt ttataagctt catttacaat
    36481 ttcttgtttg aggcaagaac atttttctcc attaggtaaa aatcctttat ctttacaaga
    36541 aatacattga aactttattt ccaagtaatc aagaggtatg ttactttcag ctaaaagtct
    36601 ttctttcttt acttttaaag actctatatt ctctttagtc ttctttacaa tttcatcttt
    36661 agacttagga tttaaaagta ctatcttagc taaactgaga cctattttag aaatttcatc
    36721 atctatagat tttatctcag ggattctatt atatacctca ttttttctat gttctaataa
    36781 gagttcatta tcatctcttc tcttagcata tttagcaagt atttttctta ttttatcctc
    36841 attcatttaa tcacctactt aaacttttct ttttgacttt ttttaataat ctcatcgagc
    36901 tcatcagagg tatactgagt gaatgtttcg ttgaaattat gaaacttagt ttttttgtaa
    36961 gtattgttgt tttgagtagt attaatctgc ttcttattaa tattctcctt aactatacgt
    37021 tcttcctctt tttgctttaa atcatttaaa ttttttatat ttttttcatt ccaattttta
    37081 attataccat ttatataaga tatacttggg tttgaaatat tcttagactt agaacatgca
    37141 tttattatca aatctatatc catattaaat ttatcaaacc aagtgtccat aagttctttt
    37201 tctgattttg atggttgtct tgaaaatccc aattcattga aaatcgtttt atataaaata
    37261 tatcgttcac ttcttaccaa aaagctttct tcaacatctt ttggcgtata taaatttgaa
    37321 tcataccaat ttctaataat gccttcaatg tatttaactg gctttgatgt accagtttta
    37381 tctttgacgt attcatatgc gcaaagtatc atatcggggc tcatattgta tttattcatt
    37441 atatccataa tactcatctt ttcgcttggg tcgagatatc gcccaactat tttatttata
    37501 gaattaaaca tttttctaat acttggattt tcagaagtag atacaacctt atctgtatta
    37561 gattttatag atgaattatt gcttaatata ttctcaatat agaaattttt taaatctaaa
    37621 aattctattg aataattaaa attatcatat tctccattgt catgtatttt tataatcttc
    37681 ttttcttccc agaatttcca agcagataac acatcagaca atggtatatt taggtttttg
    37741 gctattgaat tattgtcgaa cttaggatta gaagtaatat cacatgcttg tctatatcct
    37801 aaaagataaa cttttacgta taacccgtcc gccattggca taaatatatc tataaagata
    37861 ttgggaatag tgatttcacc taaatctatt tcattgcttt ctttaaaaaa cattttatca
    37921 cctcagatta aattatatca ttttttatat tatatttgtc tgtaaacaaa taaaaggtca
    37981 caagattagc atgtaaatat tttctaaaag tattcatttt gaaaaggagg agatattttg
    38041 gttataaaat tcaaccaaaa agtaagaaga acattatcag tagcgattct atttatggta
    38101 ttaataatag gttgctttat agaaaaggat gacagtttag agacatacaa agaaaaaact
    38161 aattcaaaaa taaatcagta taatatggat gttatatttg atgatgagac taagaggtta
    38221 atgtgcaatc agaatgttga ctacataaat aatacaaaat caaacttaga taaaatatat
    38281 ttccatatat atccgaatgc tttttccaag aaagattttg ctccttttga aaaaagtgaa
    38341 atgacaaggg cttatcctaa tggatttaac gaaggatata tagatataaa gaatatatta
    38401 aataataata gtaaaatgga gtataaaatt aaaggagaca aaaatgacat attagaaata
    38461 gaattaggga aagagttaaa accaaatgaa agaatgtcat tagatataaa atataacgta
    38521 aaaataccaa attcaattgg aagatttgga tatggagaaa atactataaa tgttactaat
    38581 tggtttccta tagcttgtgt aaatgatgaa aggggatgga atttaaagag ttatgaaact
    38641 attggcgacc cattttatag tgagactagc gattttcatg tgaaactatt gattccaaat
    38701 aaatataaaa ttgctcatac aggaaaactt attgatgata aacatgataa taagaaaatg
    38761 ctatatgaaa tagaagcgga aaacgttaga gattttgcat ttatactaag tagcaaattt
    38821 aatgtaaaca aagtagattc aaagggagta gctatcaata catataattt aaataaaaag
    38881 ctttctaaaa aagcaactga agtggcaaaa gattcaataa atatatttag tgaattattt
    38941 ggaaagtatc cttacaaaga attttctgtt gtagcaagtg acttttttat aggggggatg
    39001 gaatacccta tgcttgttat gatagaccaa agtttatata atgaaaaaaa tgaattttta
    39061 ctagaatatg ttattgccca tgagacagca catcaatggt ggtattctgc tgttggaaat
    39121 gatgaaataa gtgaaccctg gttagatgaa gcacttactg aatattctac aatagtttat
    39181 tttgaacaaa agtatggtaa agatatggca aacaagctta taaaaactat ggagatacag
    39241 acaaaaagtt atttgagcga aaatatattt aagccagcaa atgagtataa aaattctact
    39301 gaatacagct tgaatgttta tacaaaaggc gcaatagcat ttaatgaaat aagaaaagaa
    39361 gttggagata aagtattctt tgagacttta aatgagtact ataataaata taagcataaa
    39421 aatgctaatg gaagagcctt tgtggaacta tggaatagta aaggtgtaga tataaataag
    39481 attataagtg aatgtaagta aacagtgaat gtaagtaaat tttgtgtaga gtattaaatt
    39541 ttatttaata ctctgttttt ttggtataat ataatttata aggcagaaaa agaggtgaag
    39601 gaatgctaaa gtattgttca gtagggagtg gtagtagtgg taattgccac tatatagggt
    39661 ataaaaatac taatatacta gtagatgcag gattaagtgg caagagaata actacaggtc
    39721 ttaaagacat tggtgtagat gcagataaat taaaaggaat atttataaca catgagcatg
    39781 ttgaccatat aaaaggagca ggtatattat caagaaagtt tgatatacca gtatttgcta
    39841 atataaaaac atggtgttct atgaaggaca aattaggtga catcaaagat aagaatatga
    39901 aagtatttga aaatgataaa acctactcat taggtgatat catagtaaga cctttttcaa
    39961 tagaacatga tgcgtctgac ccagttggat ataattttta tacagagagt gatgaaaaaa
    40021 tgtctatagc tactgatata ggatgtataa ctcaaaatat aaaaaaacat ctatataaat
    40081 ctaaattagt agtattggaa tcaaattatg accctaatat gcttatgatg ggttcatata
    40141 catatgcttt aaagaaaaga gtaatgtcaa gtactggtca tttatccaat gaagatgcag
    40201 ctaatttctg cgttgaactt attaatgaag gtacagaatc tatattatta gcacatttga
    40261 gtaaagaaaa taattttcct gagttagcgt atgagacttc aaaaggagta ttagcatcaa
    40321 atgatatagt agttggacaa gatgtaaaac tagatgtact atcacgaaat gatgtttcta
    40381 atgtttatga gatgaagtag aggtgaataa ctttgctaaa gagaaatttt gaaaatgttg
    40441 aggataataa aattttatac tctataaagc agggtatgat tttagcgata cctgctatta
    40501 tgactggttc aacggctctt gtaattttga atcttccaat aaaggcttat caagaatatt
    40561 tatcaggctt gtttaatgga gaggttacaa atattcttaa ctttataaat gattctactt
    40621 taggaattat ttcattaata atattattaa caataagcta tagttatgga aaaatatatg
    40681 ggagcaagta tacagtactt gttcctatag ttgcaatgtg ctctttttta gtattttcac
    40741 atggtaatga gcttaattct tatatagaaa tatttaaaac aaagtggtta tttacatcta
    40801 taattgtttc tatgacatct tctgtattgt ttgtaaaact tacagaatct tttgttacaa
    40861 gtgtaaagtt tcatacagaa ggggctgatg cagactttaa tatggtagtt tcggctatag
    40921 ttcctttcat aatagtagta tttttgtttt ctatttttag agtgataatg ataagtctaa
    40981 ttggcagttc aaactttcaa gatatatttt ataatttatt ttcaaatgtt tttaataaaa
    41041 tgggaacaaa ccttatgagt gctctgttgt ttattttttt aatgcacttt atgtggtttt
    41101 ttgggattca tggtagtaat gtattagata ccgtcgcaaa aaatttattt gaaaatagta
    41161 tggcaattaa cataaattta gttaataata atcaattacc aacagaaatt tttactaaga
    41221 ccttttttga tacaatggtt ttattgggag gatgtggctc tttactatgt ttagtaattg
    41281 caatttttct tagtgaaaaa cgtataaatg taagaaagtt agctaaaatt gcttcaatac
    41341 cagctctttt taatatcaat gagatgctag ttttcgggat accaattgtt tttaactata
    41401 ttatgtttgt accttttgta attactccta taatcttaac catcacaagt tatgtggcta
    41461 tgtctacagg tattgtacca tgtacagttt cagctgtaga atggacctca cctatatttt
    41521 taagtggata tatgtctaca ggttcaataa gaggtagttt gttgcaagta tttaatttat
    41581 gtgttggtgt tatgatttat attccgttta taaaaatgtc gcaaaataga tatacatata
    41641 tgtttaaaaa taatatagaa aatttaacaa acttattaaa aaggtatgaa ttaacaggtg
    41701 aacagcctaa tcttctaaac cataatggta aaataggttc aatttcaaaa atgttggcat
    41761 cagacttgaa gtttgctatg aaaagaggag aaatagagct tttttatcaa ccacaagtca
    41821 attataatgg aaatgttgtg ggagcagagg gattacttag atggaaacat tcaataggag
    41881 ggttcattta tccaccacta gttataattt tggctaaaga agaagggttc ttagatgagc
    41941 ttggagagta tattattaat aaagcatgta ttgatttaaa gaagagtgaa aagctattga
    42001 gaaatcctgt taagttctct gtaaatatat ctccagacca acttgatgac cctaagttac
    42061 cagagcaaat aaaaaatatc attagtagta atgatataaa tcctaatatg ttaggtatag
    42121 aaataacaga gcaaattgca ttatcaggtt cacaaataat tattgaacgt atcaatgcga
    42181 tacataactt gggaataaag ttaattatgg atgattttgg gatggggcat agctctctta
    42241 tatatcttca aaataataac tttgatatag ttaaattgga tggttcatta gttaaagaga
    42301 tacttacaaa taaaagaagt agtgaaatta taatgactat tgtaaatctt tcaaaaaatt
    42361 tagattttga tatagttgtt gagtatgttg aaaatataga ccaaagggat aagttatatg
    42421 gtttaggatg tgaaatatat caaggatatt attatagcaa agcaatacct tttgaagagt
    42481 ttgtagaata tgcaaatcaa gaaagagaga tataatttta taagagcttg taaattctag
    42541 taatgcatag atttgtaata aaattaagtt taggagaaat atatgaatat aagtatagta
    42601 gtagtaggta aaattaaaga aaaatattta aaattaggta ttgatgagtt caagaaaagg
    42661 ctttctaagt attgtaagtt agaaattata gagttggatg atgaaaaagc tccagagaat
    42721 ttgagtgtta aggaaatgga aattataaaa gacaaagaag gaaagaaaat tttagggaag
    42781 ataaagcata atagttatgt tatagccttg gctattgatg gtaagggttt atcctctgaa
    42841 gaacttgcta agactatgag tgatttagca gttagaggta atagtagtct ctgttttata
    42901 ataggaggtt ctctagggct ttctgatgag gttttaggaa gggctgatta taggttatct
    42961 ttttctagga tgacttttcc tcatcagatg atgagattga tattgttaga acagatttat
    43021 agggcttata ggataaataa tggagaaccg tatcataaat aaatgatacg gttttttatg
    43081 atataattga attataaaat aaaatcaaaa tgtaaagtag gtgtagtaat ggacagacta
    43141 gatatattta tcgaaaatta tgattttaaa gaatatcttg aaaaattacc tagagaatta
    43201 atagaagaaa attataaaga ttttgttata gaattttgga aaaatcctag tgtagatatt
    43261 attaaactaa aatcaaaata tagtataaaa aatgtagtat ctataagtag tatattaatg
    43321 caaaatccta tccaagagta ttatgagttt cttgaagata tttggaaaaa taacataaaa
    43381 atagatgaat taattaataa gtatttaatt acagcaacaa gaatggatca aattaaattc
    43441 ccattaatat atattgatga agattctttt tgtccttatt gtttaggtaa taaattcaat
    43501 attattaata atattaagca acaagatata aaagttgaat gtgaaaagtg tggtgaggca
    43561 attagttatt ttattcataa ggaagaagta gataatataa tagaagaaga aaaaagaaaa
    43621 aaagaactat ttataagaac attggagaat gtatataata aaataaacaa gattaaatgt
    43681 catagatgcc aatcagattt atatgtgtgt attaatgata aaataatgga gtataatatt
    43741 aggtgtgata gatgtaattc acagtatgaa tcggttgaaa ttgcacagga acaatataga
    43801 ttatggcaac aaagagctgc aatgatggct aacataaaaa ataaagaaaa tgaattgttg
    43861 ctaaagacac tatcaattaa ggacgaaaaa gatattaaat ttaaactcga agatataatt
    43921 acggagagtg aaagtataga tgtaattagt gatttagtag atatgcaaga tagaggagag
    43981 aatattttca agcaattatt taataattat gttagactag gaagattaga cagaaggtta
    44041 ttggtaactg tgtgtaaaga ttgcaagcaa aatgaaaata cttatacatg ggaaagaggc
    44101 aaagaacatt taataaatat gagggggatt acatacagtg aagcagttgt atatgagtta
    44161 atggaaaaaa caaagctatt gccaataaga gcaattatta gaagacttat ggagaaaaaa
    44221 ctattgtatt gtgatgaaag gagcaataat ttacaagtac accctgtttt gattgaaaat
    44281 ttagctaaac tagaatcaat aaataaatct caaaatataa ataatcaagt agcttatcta
    44341 atattcaata agcaaaaata tatatgttat cattgtggtg aatcaggaag acccttaaag
    44401 ataggatatc taacaataga taaaaatata aatgatttat caagtttagt gggaatatgt
    44461 gatagttgct atgaagatat tactgaagat gaaattatta tagataatat tttttgtgtg
    44521 gtagatgaaa gaaacaatga attaggggaa gcttggaaat ttttaataca atatgatagt
    44581 agttttagaa ataatacaat tgcagaagta gagcatgcaa gattatatga tatgtatgat
    44641 gaagataatt taattcgtgc gtatgcaaat acattgtata aacaaacgaa agatgggttt
    44701 gctaatgaaa aagtattttt tgcatatgca agagcaatat tgaaaaatag caaagggcaa
    44761 gtaagtgtac tacaaatagc aaaagaaaaa tttaaattag aaaagtggtt aaatggataa
    44821 agggataata catattaaaa ttataaattt atataaaact gtattgcaat taagcaatac
    44881 ggtttttata tataagataa tgatacaatt aaccaaaatg tggtagtaca taagtgttat
    44941 gagatttaaa tattagcgat taaaataatg attaagtaaa attgtaattg atgaggagta
    45001 ttaacgtatt agtttatttt tatatataat taaaatatag gagggaagaa tgtgagtcat
    45061 agtaaactat ctgatcacaa attaaaaaag gggaaattta tagcaccttt taatgaattt
    45121 atgacaccat tgaatagtaa tgagtcatgg tgccttggtc gattgccaga gtatatatgg
    45181 ataggattat taataaataa atatggtaga aaaaaaggga tggaaataat ttataaaata
    45241 attgtaaaac ttcattcaat tgaatcagat attgtaacac ctaggttgtc tgagatttta
    45301 tctatggagg atgtaaagca ggatgaattc tttagttata ttctaactat tgttgaaaag
    45361 gaagctttat ctccattgac attaatattt acatatagta attatcctat ttttgttaaa
    45421 tattttgcag agccaagtat aaaaattgaa gatagggtta cctatttaga tgagacttta
    45481 agaaaagcta tgcatcatca aagtgaattt gctacagaca taagatttat agttttatat
    45541 tatttttgta tgtcaggaaa actcgtattg caaaaagaac agattaataa tttgctggaa
    45601 tatcctaatt tagaacacac tgatgaaaaa atgagaatgt tacgtccgtt tattcgtgca
    45661 gcagaattaa tggttttaaa tatggaaaaa aaatctgatt ggtatttaga tattttttgg
    45721 gggaggatta gtgttatgac tgaatgtaaa ttatacgcaa ctaaatttga agatgaaact
    45781 gctaatacag acttgtatat ggaacaatta catgagattt ttgtttattt atcagattta
    45841 ctatgtaaag taaatccatt agacgagaaa atgttagtat tacttggaat agcaatatat
    45901 tcttataaac gattacgaga agtagttgag cataaattat ttaattccat ttcaggtaga
    45961 ggctcagtaa gggtattgat agaaaattat attatgatga agtatttagt aaaaatagaa
    46021 gctcaacatg aaaatatatg gtcagaatat caattttatg gaataggtca atataaatta
    46081 attgtggcta gatatagaga acaagaacaa gatttaaaaa atagtcatgt tgattataaa
    46141 tatattgaag ctttagttaa tgaatttgta attgaagaaa caatagatat ggatactaaa
    46201 tattttgata agcaaaatat acgtagtaag gctgaaatag ttggagaaaa agagttatat
    46261 gggttgtact atgattatga ttccgcatat gaacatggtt tatggggatc aataagagaa
    46321 agttcaatgt taaagtgtga taatccagct catcactatc attgtatacc agatgtagac
    46381 aataatcaaa atttaaaaag tgtttggcat gattgtgtaa acataatgga aaaaacttta
    46441 cttttcttag atactgtata tggtatacca caacatttgc tattagaggt gataaaaaat
    46501 ggaaaagaat tttttactaa ataaaataaa tagtattcag aaggaatatt tagaat
//
LOCUS       DAEOSP010000006        46462 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.006, whole genome shotgun sequence.
ACCESSION   DAEOSP010000006 DAEOSP010000000
VERSION     DAEOSP010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46462
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.006"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(213..626)
                     /locus_tag="KRH54_000254"
     CDS             complement(213..626)
                     /locus_tag="KRH54_000254"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBG8577934.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(667..1797)
                     /gene="rodA"
                     /locus_tag="KRH54_000255"
     CDS             complement(667..1797)
                     /gene="rodA"
                     /locus_tag="KRH54_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBG8577935.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(1882..2166)
                     /gene="minE"
                     /locus_tag="KRH54_000256"
     CDS             complement(1882..2166)
                     /gene="minE"
                     /locus_tag="KRH54_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBG8577936.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(2184..2981)
                     /gene="minD"
                     /locus_tag="KRH54_000257"
     CDS             complement(2184..2981)
                     /gene="minD"
                     /locus_tag="KRH54_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBG8577937.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(3005..3688)
                     /locus_tag="KRH54_000258"
     CDS             complement(3005..3688)
                     /locus_tag="KRH54_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBG8577938.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(3786..6764)
                     /locus_tag="KRH54_000259"
     CDS             complement(3786..6764)
                     /locus_tag="KRH54_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBG8577939.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(6776..7264)
                     /gene="mreD"
                     /locus_tag="KRH54_000260"
     CDS             complement(6776..7264)
                     /gene="mreD"
                     /locus_tag="KRH54_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBG8577940.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(7279..8172)
                     /gene="mreC"
                     /locus_tag="KRH54_000261"
     CDS             complement(7279..8172)
                     /gene="mreC"
                     /locus_tag="KRH54_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBG8577941.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(8182..9192)
                     /locus_tag="KRH54_000262"
     CDS             complement(8182..9192)
                     /locus_tag="KRH54_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG8577942.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            complement(9313..9972)
                     /gene="radC"
                     /locus_tag="KRH54_000263"
     CDS             complement(9313..9972)
                     /gene="radC"
                     /locus_tag="KRH54_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBG8577943.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(10017..10601)
                     /gene="maf"
                     /locus_tag="KRH54_000264"
     CDS             complement(10017..10601)
                     /gene="maf"
                     /locus_tag="KRH54_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBG8577944.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(10821..11792)
                     /locus_tag="KRH54_000265"
     CDS             complement(10821..11792)
                     /locus_tag="KRH54_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBG8577945.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(11808..12383)
                     /gene="rsxA"
                     /locus_tag="KRH54_000266"
     CDS             complement(11808..12383)
                     /gene="rsxA"
                     /locus_tag="KRH54_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBG8577946.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(12398..12991)
                     /locus_tag="KRH54_000267"
     CDS             complement(12398..12991)
                     /locus_tag="KRH54_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBG8577947.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(13004..13573)
                     /locus_tag="KRH54_000268"
     CDS             complement(13004..13573)
                     /locus_tag="KRH54_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBG8577948.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(13573..14550)
                     /locus_tag="KRH54_000269"
     CDS             complement(13573..14550)
                     /locus_tag="KRH54_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBG8577949.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(14573..15910)
                     /gene="rsxC"
                     /locus_tag="KRH54_000270"
     CDS             complement(14573..15910)
                     /gene="rsxC"
                     /locus_tag="KRH54_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBG8577950.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(16126..17070)
                     /locus_tag="KRH54_000271"
     CDS             complement(16126..17070)
                     /locus_tag="KRH54_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577951.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(17181..18455)
                     /locus_tag="KRH54_000272"
     CDS             complement(17181..18455)
                     /locus_tag="KRH54_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG8577952.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDIVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEGTKEKPNSSSN
                     QNSQPNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(18587..18958)
                     /locus_tag="KRH54_000273"
     CDS             complement(18587..18958)
                     /locus_tag="KRH54_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG8577953.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(19186..19785)
                     /locus_tag="KRH54_000274"
     CDS             complement(19186..19785)
                     /locus_tag="KRH54_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577954.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(19986..20198)
                     /locus_tag="KRH54_000275"
     CDS             complement(19986..20198)
                     /locus_tag="KRH54_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBG8577955.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            complement(20271..21986)
                     /locus_tag="KRH54_000276"
     CDS             complement(20271..21986)
                     /locus_tag="KRH54_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG8577956.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(21986..22540)
                     /locus_tag="KRH54_000277"
     CDS             complement(21986..22540)
                     /locus_tag="KRH54_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG8577957.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(22533..23135)
                     /locus_tag="KRH54_000278"
     CDS             complement(22533..23135)
                     /locus_tag="KRH54_000278"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBG8577958.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(23144..25792)
                     /gene="polA"
                     /locus_tag="KRH54_000279"
     CDS             complement(23144..25792)
                     /gene="polA"
                     /locus_tag="KRH54_000279"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBG8577959.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKDYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            25944..26031
                     /locus_tag="KRH54_000280"
     tRNA            25944..26031
                     /locus_tag="KRH54_000280"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25978..25980,aa:Leu,seq:caa)
     gene            complement(26218..26661)
                     /locus_tag="KRH54_000281"
     CDS             complement(26218..26661)
                     /locus_tag="KRH54_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577960.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMCYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKRL"
     gene            complement(26664..26861)
                     /locus_tag="KRH54_000282"
     CDS             complement(26664..26861)
                     /locus_tag="KRH54_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8577961.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(27053..27919)
                     /locus_tag="KRH54_000283"
     CDS             complement(27053..27919)
                     /locus_tag="KRH54_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG8577962.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(28145..29014)
                     /locus_tag="KRH54_000284"
     CDS             complement(28145..29014)
                     /locus_tag="KRH54_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG8577963.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(29520..29741)
                     /locus_tag="KRH54_000285"
     CDS             complement(29520..29741)
                     /locus_tag="KRH54_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577964.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(30243..31223)
                     /locus_tag="KRH54_000286"
     CDS             complement(30243..31223)
                     /locus_tag="KRH54_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577965.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(31387..32259)
                     /locus_tag="KRH54_000287"
     CDS             complement(31387..32259)
                     /locus_tag="KRH54_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG8577966.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITFKYEK"
     gene            complement(32423..32974)
                     /locus_tag="KRH54_000288"
     CDS             complement(32423..32974)
                     /locus_tag="KRH54_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8577967.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(32994..33896)
                     /locus_tag="KRH54_000289"
     CDS             complement(32994..33896)
                     /locus_tag="KRH54_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG8577968.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(33911..36283)
                     /locus_tag="KRH54_000290"
     CDS             complement(33911..36283)
                     /locus_tag="KRH54_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBG8577969.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(36504..37193)
                     /locus_tag="KRH54_000291"
     CDS             complement(36504..37193)
                     /locus_tag="KRH54_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG8577970.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYVK
                     FIYNLFKSLTDTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(37434..38813)
                     /locus_tag="KRH54_000292"
     CDS             complement(37434..38813)
                     /locus_tag="KRH54_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG8577971.1"
                     /translation="MLNFMTELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGNYFSNINYLFYILAFIAWGGYYNYFYKMGYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPYLESIKIQIYSNLIIILIYLIFSFFRKS
                     IKLISQDKRNYVYLLFALLVNTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEVCLKKGIKFTCS
                     INFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENINKYIRIKGTITRSFFVLKCEN
                     SKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPL
                     DQDTDSWGD"
     gene            complement(38807..39520)
                     /gene="rgbR"
                     /locus_tag="KRH54_000293"
     CDS             complement(38807..39520)
                     /gene="rgbR"
                     /locus_tag="KRH54_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBG8577972.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            complement(39770..40330)
                     /locus_tag="KRH54_000294"
     CDS             complement(39770..40330)
                     /locus_tag="KRH54_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HBG8577973.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKTNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            complement(40425..41228)
                     /gene="zupT"
                     /locus_tag="KRH54_000295"
     CDS             complement(40425..41228)
                     /gene="zupT"
                     /locus_tag="KRH54_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBG8577974.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            complement(41379..42542)
                     /locus_tag="KRH54_000296"
     CDS             complement(41379..42542)
                     /locus_tag="KRH54_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8577975.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAILEGTIRLFNPELRKQIPRILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            complement(42602..43789)
                     /locus_tag="KRH54_000297"
     CDS             complement(42602..43789)
                     /locus_tag="KRH54_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577976.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            complement(44152..45852)
                     /locus_tag="KRH54_000298"
     CDS             complement(44152..45852)
                     /locus_tag="KRH54_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8577977.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            complement(46107..46382)
                     /locus_tag="KRH54_000299"
     CDS             complement(46107..46382)
                     /locus_tag="KRH54_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8577978.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
BASE COUNT        14576 a         8701 c         4839 g        18346 t
ORIGIN      
        1 ttgattctat ttcatattat taaaatagag taatagttta aattctacta ctctatctat
       61 aatctatttc ctaattattt aatctcaaat ttagctcttc atcatttaaa tctttcataa
      121 gatttctaca ggtactagat ttattccaac agaaagacta gggcattaaa cacttatata
      181 tctgtttctt tagactgtaa tattaatatt ttttaaatac gttgactttt gctttttcta
      241 acttctctcc atgatagttc tcctctatca agagctttta taaatatttc tgctgaagct
      301 agatttgtag ctattggaac atgataaaca tcacaaagtc ttattaacgc ttgtatatct
      361 gtttcatgag cctgagatgt caatgggtct cttaaaaaaa taactaaatc tatttcttgt
      421 gttactatta aagaacctat ttgttggtct ccacctaaag gaccagatgc taatctattt
      481 atatttaatt cagtttcatc cattattctt tttcctgtag ttccagttgc atatagtcca
      541 tactttttta aaattgattc ataacctata caaaatccga ccatagtgtt tttcatttgg
      601 tcatgtgcta ctaatgctat attcatatat ctaatctcct caatttatgt ttagatgtat
      661 ttaaatttag aaatttattt tctttcttct catacacaca tttagtacta atcctaagtt
      721 tgctaaagat gtcaataacg aacttcctcc ataacttaca aaaggtaatg ttactccagt
      781 tactggtatt aacgcaaccg tcatacctat attttggatt atctggtaac caaacattcc
      841 cataacacca accactatga gggttccata aaaatctttt gcatctcttg caattgctaa
      901 aagccttaat aggaatatca taaataagat tatcaacact gccattccta ttacaccaag
      961 ttcttcacct acaacagcaa atataaaatc actatcttgt acaggtaaaa aatcttcttg
     1021 attttgactt ccattgtaaa gtcctttacc agttacgcct ccagaaccaa ttgcaatcag
     1081 agattgcata acttgatagt ttccttttag agttacatct tctggattta gaaaagcttc
     1141 tattcttacc ttttgatggt ctgccatcat taaatacatc agtggcattg acactaatag
     1201 tatgattatt cctcttttta tcaactttga gctaagacct gctgtgaaca gcattgcaaa
     1261 tatcatacaa caaaatacaa ttcctgttcc caagtctggt tgtgcaatca ataatccgat
     1321 aaatggaaga gaataaacta caacaggcat aacttccttt aacgtattta atttatcctt
     1381 tttggattca agtatctttg cataacttag tacaaatgtt aatttgacta tttcagatgt
     1441 ctgcaagtca agtggtccta agttaatcca cgacctagct ccacctttta ctgtcccaat
     1501 tccaggtaat aaaactatgg ctaacaaaat aagacttatt atataaagcg ctttataata
     1561 cctgcctaga aggttatagt caaaaaataa tatgactata atcattccta tcccaagtac
     1621 aaaagctaat ccttgtttaa tcaattgatt atatgaacct gttgaattag catgtgttgc
     1681 actactaagt ataacaagcc caaaaataaa gatagctaat actgtaacta taagtttcca
     1741 gtctaactgt tttatcaact taattgtcga cttataatta atattgattt ttttcaaaga
     1801 atcaccgtct ttctatcttt aaacatataa ttaaataaaa ggggcatata ccaataagga
     1861 tatccccctt ttaagcacta attacataca tctatctttt atattcttta gtggtatatt
     1921 agcaactaat gcagatacag gtttgtcatc atgctcacct ctgcatttag acatttttat
     1981 ttcaagacca tcatcttcta tttctgcata attagctatt acttttaaaa tgtctgtttt
     2041 tatcatctct aaaagttgtg gtgaacaatc tactctatca tgaactaaaa ctagctttaa
     2101 cctctcttta gcaacagatt tactagtttt agcctcgtta gaaaaaactc taaataaatc
     2161 taacacagat gtatcctcct ttgctactta gccatgctaa acattttttt aagtctacca
     2221 aagaatccat cttcaacttc aagatcaagt agaggaactt cttcatttag tatccttttt
     2281 gcgatatttt tgtatgcttg accagcaagt gacttagaat caagtatagc tggctctcct
     2341 ttatttgttg atataattat gctttcatca tcaggaacaa gacctaacaa atctattgct
     2401 aatatttcta ttatatcttg tttatccatc atgtctccac gcttaaccat atcatttcta
     2461 attctattta taactaatct tatctctttt atttcatttg cttctaatag acctattatt
     2521 ctatcagcat ctcttactgc tgatatttct ggattagtaa ctacaatagc tctatctgcc
     2581 ccagctactg catttttaaa gccttgctca atacctgctg gacaatctat tattatatac
     2641 tcgaaagatt cttttagttt ctcacacagg tcaatcatct gctcaactga gacagcattt
     2701 ttatctctag tttgtgctgc tggtaataaa tatagattat caaatctttt gtcttttatt
     2761 agagcttgct taagcctaca agttccttca actacatcta ctatgtcata aactattcga
     2821 ttttcaagac ccattactac atctaagttt ctaagtccaa tatctgcatc cacaactact
     2881 gtttttttat tttctaggct tagcgcagtt cctaagtttg ctgcggtagt agtttttcca
     2941 accccacctt taccagatgt tataactata acttcgctca taatactacc ccctattttt
     3001 ctttctattt gtctaacttt gacaaatagc tttcgattac aattcttcca ttacttacaa
     3061 aggctatttc tgggctaatt tctgattcac tttcataatt ttcttcatca tctggagctt
     3121 ctgcaatatt atctgctatt tgtaatattt taggattaag atttccagca acaacatatg
     3181 caaattcatt gccttctgca cctgcatgta caaaaccttc tattttaccc attacaaaaa
     3241 cattacctcc tgctacaacc ttagcaccag catttatatc tcccataaca actgtatttg
     3301 attttgaaag gacttttgaa ccaggtttca tatcattcaa tataactaca tcaccttcaa
     3361 attcaatatt ttcaccagat cttagggtat tgacatattt tgttggaaaa ttactatttt
     3421 cttcaatttt ttgatcttca acaaattcca catcaaatct agaagtaatt ccttctttaa
     3481 gttctagaat ctccatgtca gttaaacaat cactattaat tttactaatt ttagccccat
     3541 taaaaaagcc aacatatgct tccaatttgt ttattatttt ttcctgtatt tcctcaaatg
     3601 gagcttctct ttttatattt actattatcc ctcttttatt acccttgaac tctactagtt
     3661 cttgactaca aatttctctt aaagacataa caacctccag agtgatatat catttatatt
     3721 cttcaaatac gctttaaacc cttttatttt ttctatataa agctaaagtt tatacataga
     3781 ttgagctatc tattaatttg tgactcgaaa tttatatttt cgtcagaaga attgttaaca
     3841 tcattcgtct tgttgtcatt tttactatta gttggctcta atcccatata agttccaata
     3901 acttctctag ttggtaaaag tgagaataca cttgaatcac cttgaggaat tacacataca
     3961 acagctattt ctgggtcatc tgctggagca aaagcaacag tccacgtaaa agaaccataa
     4021 tcttgtttaa atctatctat ttggtcatct gttatttttg gatttaattc ttttatagct
     4081 tttcttagat atgaagaatt caccttatca gtgtcctcta atctaacttt tactccatct
     4141 tctaattctt cctctaattt ctttctttct tcgtctttca aatctttatt ttctaatttc
     4201 ttttttattt ccttttcctt agctaatgaa agctcttttt ctttttctgc tttcatctta
     4261 tcagctagtt taatcgcatc atttaggttg acattataag atgacatatg actttttaaa
     4321 tattcatact cgttatctgt aggtattttc ccacttttct cagctgtacc agtttttgct
     4381 gctacatcta ttggaaaatc agcaaaagca ctcttcgctg taccttgtct tgcaaccaat
     4441 ttcatacctt ttgttaattc ttttaagtta tctggatttt taaaaggtat tttttcgact
     4501 ttcttctggt cattaatttt aactgatgaa taatcacttg aaacagctct atcaactaca
     4561 gataattcta ctaaattacc tccatttgct atagcagcta catatcttga tatttgagct
     4621 ggtgtataag cattttcacc ttgtcctata gctaagttga atgtatctgc agtactccat
     4681 tttgcaaaat taaaataact aaatactgct aaatctgcta cttcttcaat tctatcttct
     4741 ttaacatcca atttttttag cctattcatt gtttcaactc taccaggagt tttcttttct
     4801 gctgcccagc ttacaatctc attgattctt ttctcatatt ctttaggatt tttttctcta
     4861 gttatatcaa caaaatcatt tgccatttct ctttctaaat ttgactttag aagagcttgt
     4921 gttgatttca atttatcttc tgtgctagga actttacctg ctctttcttc tacttcatct
     4981 tgaagccctg tattttggtc aagcccaaat aattttgcat actcaagtat tttatttgca
     5041 ttcatatcta taccaatgct ttttccatct ggccatgttt tacctgtacc aattgttgcc
     5101 atatatatat tacaagactc ttgtattgct ttatataagt ttgtcatacc atggtttcct
     5161 ctaccatggt tccatacata gtcaccaaaa gattttttac ctaacattat aaccccaggg
     5221 tcatttatag taaagtttgg atccagacca ttttctaacg ctgccattga agttaccatc
     5281 ttaaatgaag aacctggctg aaattcccct tgtgtaacca agtttaaaag tggacttcct
     5341 gctagtaagt catttggatt ttttggttga agtgctttat aatcctctgt agaaatacct
     5401 tttgcaaatt tatttgggtc ataattagga taacttgatg aagctaatac ttctccattt
     5461 ttaacatcaa tagctataag agctgctgac tgtgcttttc ctgcatatgc actaattggt
     5521 ttgtccccaa atttactttt aaaagtacct cctttagaag caacttcgat tatctgtttt
     5581 aatgcattat ctgaaacttc ttgtaaatct ttatctagtg taagatacac agtatctcct
     5641 gatttaggtt ttttagattc aatttcttta ctgattctac ccaatgcatc aactttaacc
     5701 attttataac catcagttcc tcttaatcta ctttcattac tcttttctac tcctgcaaga
     5761 cctaccatat ctccagtttc ataacctttt tgaaggtatg attctatttg agtggatggc
     5821 atttttccta catatcctaa catatgagaa gctaatgacc cattaggata atatcttact
     5881 ggttctactg ctattgaaac accaacaagg tccatagcac tttcttcaat ttgagcaatc
     5941 gtagtttctc ccacatcctt agcaatagtt actggattat attgagaata tccttttgat
     6001 tttatcaaat cccttacgac cattattttt cttgcatctt catcactcaa acttttatca
     6061 atttctaatg catcactatt tcttactttt tcaaacgctt ctttggcact cagtttagtt
     6121 tcttttatct tataactagc caaccaatct ctcttatctc tttctgctgt gaacatccat
     6181 ttactaacta aaataggtgg ataatatcca ttttcattta gttttgcctg taatcttgtc
     6241 gcatccaatc ttttatcttc ttgagataat attccagctg atataagttt gtccaccaag
     6301 taataaaaac tttccttagc attataatca tttggaatac cattttctga tttatattcc
     6361 ctaatatctc tgtcatatgt ataatagtac tttccatttt ctacatatat aggaaactca
     6421 tctacatatt cttcaccatt tctctccagc aaatttataa gttttaatga tatttcattt
     6481 gctcttgaat gcttatttgc atctttctta ttaatatcgt tacctgaaac ttgaacagta
     6541 aataagtttt tatttcctgc caacagtctt ccatatctat cttttatttc tcctcttgga
     6601 gcttctattg ccaacttttt atatgtttta ttctcagcta actcattgta atattcatat
     6661 ttaaatgtag tcatatagat tatttttact aaaatagcta aaaatatcac gcccatgact
     6721 atcttaatta tttttagtct atcaacttgt ttattttctt ccatgaaatc accaactaat
     6781 cttctttaag ttttaaaact gctcttttag ataagcgata tattactaaa ccaattaaac
     6841 tattcactat tggtgctact agtagacctt ttacaaaaag tgttgatatg ttgtagcttt
     6901 gataaattat actagatgta cctatattga caattgaatc aattaaactg cctataaaaa
     6961 ttaaaacgaa agtaataata taactacctt tatatagctg atctctcatg tatccaattg
     7021 catatgcaat aataaacaaa attaatgcat ttacaccaaa aattccacca acggttatgt
     7081 ctttaataag ccctattatt gctgctataa tcccaccttc taattcatct aaataaagag
     7141 agattatggt tacatatata agaactaaat taaagcttat gccaaaaata tctatataat
     7201 ttgttatact attttctata gtcataagca caatccccaa cagacaaagt aaaacttttt
     7261 tcattgtttt tatccccttt atcctatatt tcttggttct atgactacaa cgtcatttat
     7321 attcttaaaa tcaacataag gttttacaac tacatatttt aatgacttat ctttatcatc
     7381 aaccactttt tcaacttcgc caattggtat accatctggg aagaatccaa gacctgatgt
     7441 tgttattata tctccttgaa ttacattata tgatgagtca aacatatatc cttgcaaata
     7501 tcctttacta ttataactat ctttattatc taaagtagta ttttgagtaa ttgtaccttt
     7561 agcattggca tcctttgcta gtttaaagct tacagatgcc ttagaatcca atagtgaaat
     7621 tgccttacta taattgttgg aaacctcata aactatgcca actaatccac taccattcat
     7681 aactatactt tcttttttta cacctgaatt ttttcctgca cctatgacaa aactctcata
     7741 ccagtttcca tcatttttac caacaacact tgctgaaata cttgtcgcct tatactcttc
     7801 ttgtacaaag ttcagtgtct tttttaactc ttctagcgat tctgttttat ctagtttggc
     7861 atttaaagct atcatatttt ttttaagttt ttcattttct tcttctaatt tttttacctt
     7921 tttagaatta tctttaaaat ttgctacatc tttaaaacca tttttgataa aagaaaatcc
     7981 atcatttatt cccttaccta cacttgtaat agcatctgaa gctactccta aatttactgg
     8041 actaccacta gaaaatttac caattgatat acccacaatt ccaattaaag tgatagcaac
     8101 tacgcccgtt gctatcactt taacattaat cctttttttc tcatttttgt tcttgtcaaa
     8161 tctcaaggcc atcaccaact tctaattctt agtattcatc attaatactt tttcaaagat
     8221 ttcttggtct tcaacagatt tacctgttcc aagtgcaaca caatcaagag gtgtttctgc
     8281 tatttgtaca gacataccag tttcctgtgt aataagctta tcaagacctc taagcaaagc
     8341 gcctcctcct gttagcatta ttccgttttc cattatatct gaggctaatt ctggtggagt
     8401 tctttctagt gtagatttta ttgcatctac tattgaactt acaggttctt ttaatgcttc
     8461 tctaacttct gtagagcata tttctattgt tttaggtaat cctgagatta aatctctacc
     8521 tcttatttgc atatttattt cttcatcatc tttaaatgta gaacctatgt taatttttac
     8581 attttcagca gttctttcac ctatcataag attatattct ttttttacat aagccactat
     8641 agactcgtca aattcgtctc cacctattct tatagattta gcagtaacga ttcctcctaa
     8701 agatattaca gctatttctg aagtacctcc accaatatca actaccatat taccttctgg
     8761 ttcttcaact tttaatccag cccctatagc cgctgccatt ggctcttcta taaggaatgc
     8821 atctttagct cctgcttgtc ttgcagcctc ttctatagct ctcttttcta cttctgtaac
     8881 tccaaaagga acacatatag ctattcttgg acttacaaca ccttttttat cagccgcttt
     8941 ttgtataaaa tagctaatca ttgattgagt tatgtcaaaa tcagctatta ctccatcttt
     9001 cataggtctt atagctacta tatttcctgg tgttctccct atcatttttt tggcttcttc
     9061 acctactgct aatacctctt tactatcatc tcttattgcg acaactgatg gttctctaac
     9121 aacaatacct tgtcctttta tgtaaactaa tgtatttgca gtccctaaat ctattcccat
     9181 atcctttgtc atcttattaa aactaaaaaa accttttttt tctttctttt tttccttagc
     9241 catgatttta ctccttccta ctattaagaa tcatgtttaa aacattctcc tattatttta
     9301 tcacatataa agtcaaatta tcatattttc tttaaagcta aagtataatc catctccaat
     9361 aattatatgg tctattattt ctattccaat tatttctcca catttaataa gtcttgatgt
     9421 gatattttta tcttctcttg atggttctac acttcctgaa gggtgattat gcatcactat
     9481 tatcttactt gcacttcttc ttatagcttc tttaaaaacc tctcttggat ggactaatga
     9541 cgagcttaaa gtccctatag atacatctac atcagatatt atctcatttt ttgtgtttaa
     9601 aagaaccgct ttaaaaacct ctttttttaa ataccttagg ctatccatat aatacctctg
     9661 tatatcccaa ggattcctta ttttatactt tataggttta aaactagcta ctctagagcc
     9721 tagttctaaa gctgctttaa tttgggttga ttttgataac ccaattccat ctatattaca
     9781 cagctcctct attgtcatat cttctaaatg tctaataccc tgaatatctc tatttattat
     9841 atagttggct aattcaatag catttttatt cttgttgcca gttcttaaaa gtatagccaa
     9901 caactcagca tttgataagc tttttacgcc tttggcaagc atcttttctc tgggtctttc
     9961 ctctggtgcc atttctttta ccttaatggt tgaattaaat gactttttca gataaatcac
    10021 actccataaa aaagatttat actaaaatat ttttttaata ggtcgcctaa ccttgaaatt
    10081 ggaagaccaa ctatattaaa ataatctcct ctaatttctt caactaacaa agctccataa
    10141 ccctgtatac cataggctcc tgctttgtca aatgactcat tagttttcag ataatcctca
    10201 atatcctgct ctgataacgt tttaaatttc acattgctga ttacatagtc aattaccttt
    10261 ttatcttcgc acaagtttat taagctaata cctgttatga cttgatgctc tcttccagat
    10321 agtcttttta acatatctct tgcacaagac tcatcttttg gttttcctaa aatagcattg
    10381 tctaaaacaa caactgtatc agctccaata acaacatctt cattatgttc agatgctaca
    10441 gacatacttt tttcaaaagc taatctcata actaaatgtt ttggtgtttc accttccaat
    10501 ataatctcat caatttcatt tttaattata tcaaatctta cattcgtatt ctctagaatt
    10561 tctttccttc ttggagaggc tgatgccaag attatattca tttattcacc tcatttgtac
    10621 gtaatttgta cctatttagt ctatcactat attgtaaaaa attcaacaga aatgtcgaac
    10681 cttttttgtt acactattgt tatatttatt accattagaa taaatataca taaaatagag
    10741 gtatggaaat tattccatac ctctgtttta tgtactcatt agagatatgg aaattattcc
    10801 atatccttat tttgtccata ttacaaaatt ttaatagctt tctttggaca tttttccata
    10861 cataaatggc atcctacaca tttatctgca tctaccttat gtttttcttt taattctccc
    10921 tctattgcat caaacttaca ttgttttttg cacactgtac atccaacaca aagttcttgg
    10981 tcaatagtaa ctttcttttt cttagttatg tcacctgata ttacttttgt agggcatttt
    11041 gctacacatt gcatacaacc tacacactta ttagggtcta tcttagctat tttgtcttca
    11101 aatattattg catcaaattt acaagctttt acacacattc cacagccaat acatccagca
    11161 gtacattttt ctttaacagc tttaccaaac tctttgctat tacactctac aactacttct
    11221 tgactttctg gttttgtgat tattataccc ttaggacaaa cttctttaca ttttccacaa
    11281 tttacacact tttcttcatc tattactgct attccatcaa ctatagaaat agcatcaaat
    11341 ttacaagcat ctttacatgt tccaagacct aaacatccaa atttacacat cttagcacct
    11401 gagtttaaaa catttgctgc tctacaatct gatattcctt catactcata tttatctttt
    11461 gcacttgaac aagttccttt acaaataact tttgctactt gtttctcacc tgcttcagca
    11521 cttattccca ttatttctcc aacttttgca ccaacatctg ctccacctac agggcaacca
    11581 ttaactggtg cattgccttc aactatcgca tttgcaagac ctccacaacc tggaaatcca
    11641 caacctccac agttggctcc tggaagtact cctagtatag cttccactct ctcgtctact
    11701 tctacagcaa actttttaga tgcaaaatct agtactatcc cgaagattaa gcccataatt
    11761 cctaaaacta atacagctgt aagtatcacc attttcaccc ctctttttta tagctttata
    11821 agtcctgtaa atcctaagaa agctattgac attaaacctg ctgatataag cgttataggg
    11881 aaacctttaa atgcttctgg tatatcagct aattctagtc tttctcttat acctgcgaat
    11941 attactattg ctaaagtaaa tccagctccg gctccaaatc cgtttattat tgtttctact
    12001 aaattataac ttttttgaac attaactaga gctataccaa gaactgcaca gtttgtagtt
    12061 ataagaggta aataaactcc taatgcttga tataaagatg gactcatctt ttgaattacc
    12121 atttcaacga actgaactat agaagctata accaatataa aagctattgt ttgtaagaat
    12181 ccagttccag tttttactaa taaaatctgt ataaaataag ttataattga agctagagtt
    12241 aaaacgaacg taactgcaac tcccatccct actgctgtat caaccttctt agaaactccc
    12301 aagaaaggac atatacccaa gaattgagat gttataacgt tattaacaag aacgatactt
    12361 aaaaataaaa gtattaaatt catttattac acctccacta tgctttttta cttcttaatt
    12421 tattaaatcc tgcaaataag aatcctaatg ttaagaatgc acctggtggt aatataaata
    12481 ttaatactgg ttggaaagat gctccaaata atgtcattcc aaataaacta ccatttccaa
    12541 gtaattctct tactgcacct attattgtaa gtgcaacagt aaatccaaga ccttgaccta
    12601 caccatcaac tattgaaggc atagctcctg ttttgaaagc aaaactttct gcacgagcta
    12661 atattaaaca gttaacaact attaatggta tatatatacc aagagcctta tcaagagcag
    12721 gaacatatgc ttttaaaagc atacctacta ttgtaacaaa tgtagctata actactataa
    12781 atataggtat tcttatctta tctggtgtaa tttttcttat taaagatata actaaatttg
    12841 cacatattaa aactgctgct gttgaaagac ccataccaat tccatttatt gctgaagttg
    12901 taacggctag tgttggacac atacctataa cttgaacaaa tgttgggttc tcatctatta
    12961 gcccattttt aaatacttta gctaaattca taattaaccc tccttattta ttaagagcac
    13021 tattgaaaac atctatagca gcatttactc ctgtagtaac tgctgtagat gtaatagttg
    13081 cacctgatat tgctaatatt tgattatctg ctgttgtact tccttttgct acttcaagag
    13141 gctcagcagt ttttcctttg tattgaccct taaatgcatc atccttagct tttgctccaa
    13201 gacctgctgt ttctgacata ttaccaatgt caacacctgt aacttgacct tcagatgata
    13261 tacctaccat aacttcaatt tctccaccat atccacttgg tttagctttt aaagtatatc
    13321 caaccacttc agaaccatct aaaccttcat acacttcagc tataagacca tctccaaggc
    13381 ctttgtaagt atcttccttc atttctttaa attcttttgc ttctggaagg actgcttttc
    13441 taagttcatt atttgcttgt atatttcttt gttctattac tggagctgta acctgattag
    13501 tagctccaag agctaatgca gctattgcac ttatagcaag taaagtacca cctaatctca
    13561 ccatactgtt cattatttag ccacctcccc aaatactcta tttctaacaa acttatctat
    13621 aagtggagca agacagttca ctaatagtat tgagtaagat acaccttctg gatatccacc
    13681 atacattctt ataacagttg ttataagacc agctaataca gcgtatatta tttgaccttt
    13741 tttagtcata ggcgaagttg tataatccgt aagcatgaag aatccaccta acataagtcc
    13801 tccagcaaac agttgatata ttgcgaaagt tatattaaat ccacctaaaa ggaatgttaa
    13861 tataaataca gttgctatgt aaaatactgg tataacataa tttattatgc ctttatacat
    13921 taggtatact ccacctaata ataataatat tgctgatgtc tcacctatac atccaccaat
    13981 attccctatc atcatgtcag caagactaat tccattgcta gaaagatctg ccagtcctgc
    14041 tgctccattt ttcaaaaagc ttaatggagt tgctgtagta actgcatcta aatttttacc
    14101 tatccagtta acacctggtt gagtccaagc agtcattgct actgggaatg atgctaataa
    14161 aaatgctctt gcagcaagtg ctggattgac aaagttacat ccaattccac caaataccat
    14221 tttaactact aatattgcaa atattcctcc tactagacac atccaccaag gaagtgatgc
    14281 tggtacgtta aatgctaata ataatcctgt tacaacagct gaaaaatctc ctatagtact
    14341 tttttgcttc gtaaattttt gatataaaaa ttcagtacct actgtaccaa gtatacaaaa
    14401 aaacatagca ctcaaagcat taagtctaaa gaagtatact cctgctactg ctgctggaag
    14461 gagtgctata ataacttgtt tcattatata tgaagtatct tcattacttc tcacatgagg
    14521 agaagatgat actatcaact tattttccat cgtttttccc ccttaacttt ctttatttct
    14581 gagcagcttg ctttcttctc tttgctccta tttctctctt tgctgatctt atagattgaa
    14641 gtaatggtct tcttgctgga caaataaatg agcaagaccc acattctata caatccattg
    14701 ctctatgata ttcagctgtg tcatagtcat cttttaaaga atatgcactt atgaaaagtg
    14761 gttgtaagaa tgatggacat acatctgtac atcttccaca tcttaaacag ttttgaatat
    14821 ctggagtacg tgattcttcc tcatccaaac ataaaattcc tgaagaacct ttgttagtcg
    14881 ctatttcagt agtatattga gctattccca tcataggtcc acccattata accttaccaa
    14941 tcttcttatc ttctttaaat ccaccacatt gttctattat ttctgaaact aatgttccaa
    15001 cttttgttat taggttcttt ggctctttga tgcaactacc tgttattgta gtgattcttt
    15061 caaccaaagg catacctgtt ttaatagact tagctatctg tgctgctgtg gcaacgttgt
    15121 caacaactgc acctgctgct attggtaatc caccagatgg aacttctttg cctgtacacg
    15181 cataaataag ttgtttctct gctccttgtg gatatttcac ttgaaggcca acaacttcga
    15241 tttcgctata gtctttagct acattttgaa ttgcttctat agcatctggt ttattcgttt
    15301 caattcctat aaatcctttt ttgacatcta ataccttcat taatgctctt aaaccaaaca
    15361 caacatcttc tggattttca accattaatc tgtggtctgc agttaagtat ggttcacatt
    15421 ctgcaccatt taatataact acttctgcct tgctatctgg tggaggagat acttttacat
    15481 ggttagggaa tgtagctcca cccataccta ctattcctgc ctcttttatg atacctacta
    15541 tatcttcttt gctcaaatcc tctaactgac cttttggttg aacactctca tgtaattctt
    15601 ccttaaagtc attttcgata actacacatt gagctgaacc tcctggtact tcatgttgtt
    15661 caatagcaat aactttacct gatactgaag aatgaacgtt acatgaaaca aaaccttgtt
    15721 gttcacctat tttttgccca aactttactt catcaccaac ttcaacaata ggtttagctg
    15781 gtgcccctat atgttgctga agaggaatgt aaactatctt tggagcttga gctttttcaa
    15841 gtgcctttgt gttagagtac tcttttctat atggaggatg tatccctccc ttaaaagtta
    15901 agagtttcat tgttttaccc ccttatctat aaaaaaacat tttttctgcc ttctataagt
    15961 atactatata tattaaaaaa tcttaacaat tgttaaaaaa atatccgtat ttttataatt
    16021 tattttataa atcaacacct ctatattaag tcaaattgag gaaaaggatt gcttaattaa
    16081 acaatccttt tcatatagct ttctaaagtc caaattttct gtattttatt ttatagaatt
    16141 tataaacata gagtactcct gcatggatga taaaagcaga cttttactta cactatattt
    16201 ttcttcattt gctgcttttg aagctgtatc aattttttca ttcacattct ttacatactc
    16261 tattaaatta tctttaaata aacgcatttc agaataatca atcttttcag cttcttttga
    16321 tatattttgt gatattgcct ttctatttgt caaaatagta ttatattttt ccatatctac
    16381 attattttca gcattcgacc aaaaacttga ttcttcttta aagtttgtta ttaatttatt
    16441 taattcttta gatatactct ctatataagc gtagttactt gtatactcta aagacaacat
    16501 aggtgcatct aagtgtgata aaaatgcatc tggaaacacc tttcttacac tgcttatctg
    16561 ctttctaaca tcattttcat caaaagagga ataggtctgt atttttttta ctccatcctt
    16621 ctccataact gaaaaaggta tgtcattatt aactaatgat gtctgtattt ttttcaaatc
    16681 atcattatta tctattgcag caacttgaat agtatacata tcccagcctt ctatagtagc
    16741 taatttgact tcttctggct ctttagtgtt atttaccgct ttttctggat tagtttcttg
    16801 tttatcatct gaattggttt gtgatttaga ttttttccct ttttttattg aatttaattc
    16861 atcagatata tctttataag ttatatctga tgaattattt agaaatggta ttttagccga
    16921 tacatactcc aatatcttgc tatcttttat ttttgtataa gaataatagc caattagaca
    16981 caagcaagca atcgttataa catatttacg taaatcaatc ttatgtctat tattattaaa
    17041 tcctctgtat atcgtttttc ttctattcat tagtttgtcc ctcccaaaat tatattatta
    17101 ataaagtata tttttaagag tcacaattta tgaatataaa taattatttc ttctaatttt
    17161 ttaataagaa attgtactta ctaaacaaat cttcttgcac caagtaatac tttagaccaa
    17221 tatgaactag ttaaattatc cactttaacc ttattaggat tagtctgagt accactacaa
    17281 tggataaatt tactatttcc tacatatata cctacatgat tagttgttcc atcaccatca
    17341 gtgtcaaaat aaactaaatc tcctggtgca taattcccca tagaaattgc acttccaaaa
    17401 ttagcctgtt ctctagaaac tctaggaata gaaacgccta cagaatgttt aaaaacatac
    17461 tgtgtaaatc cagaacagtc aaatttatca ggccctgaag ctccccattg atatggtatt
    17521 ccaataagag tatatgcaaa atctacgatt gttgaacctt ttgctgtaga tttatctgaa
    17581 tttccagtaa atgatggctt tgagttacta ttaggttgag aattttggtt agatgatgaa
    17641 tttggttttt cttttgtacc ttcattagtc tcagaaatat aacttgcact cacccatcca
    17701 attgttccac tcgataactt taccttatac caaccattac ttttagcttt taattcaaca
    17761 acatctccac catttaattt tcctattata gaatagcttg tcccaggacc acttcttaca
    17821 tttaaactca cagcagatgt taccttacca ttcttgcctt caattgattc ttcgctagat
    17881 ggttttttat cagaattatt agtactattt gaattactag aattgttcgt tccaacatct
    17941 tctgatgttt tagaaatata ttggctacta gcccatccag taactccatt tgatagtttt
    18001 attttatacc atccattgct ttgttctagt aactttacta tatctccatt atttgcttta
    18061 ccaactaatg aatagttagt acctgcacca cttcttacat tcagtctaga actcacattt
    18121 actttaccat ttccactaat agttgtccca cttgaagttg gattattttg acttgatgta
    18181 ccttcagacg aaccacttgt tgatatgtat tttgcacttc cccatccaac aacagaactc
    18241 gataccctta ctttatacca tccattagac ttttctaaaa tctctacagt ttttcccttg
    18301 tataattttg ctaccttatc gcaatcagaa cttggtccac ttcttatatt caaggcactt
    18361 gctgttactg ttcccttttc aagagcactt gcatttatgg agctaacaga tactgctact
    18421 gccccaattc ctaaagctgc tatagctttt ttcactacta tcactccccc caacaaattt
    18481 ttatagtcat aaattcataa cattattatc catttttagt ccctacaatt taatgataac
    18541 ttattttctt attaattgta aagaaattat aaacatatcc ttaaaattag tgatttttag
    18601 gaagtatttc aatccaataa ttatctgggt ccgctataaa ataaattccc atactttcat
    18661 tttcaaaaca tatgcaatct aaatctttat gaagtttttt tgcctcatca aactcctcaa
    18721 cttctaaagc cagatgaaat tcattgtctc ctaaattata tggtctatcc caatctctaa
    18781 gccaagttaa ttctaatgta tgactagtaa tgccatctcc taaataaact aatataaaac
    18841 ttccatcttc tgcttccttg cgctttacct ctttaagccc taaagctttt ttataaaaat
    18901 ctaaactttt ttctaaatta gtaacattga aattgttatg acaaaaatta aacttcattt
    18961 tgaaatcact ctccaatttt tatttttttt atattatatc attattataa atttatttaa
    19021 atcatattaa gttaaaaatt tgaatgatga tgaccaatta ccaaatcatg gtaaattatt
    19081 taaaagaata tgtcaaaaaa agaataagct ctttcaaatg cataaaaaat ttatacccaa
    19141 gttatactta atcatgcaaa ttggcagaac ttattcttta aaactctaac ttttaagttt
    19201 ttctttaata atataattta ttactttttt tctaatatct tcagcattat aatcatcttc
    19261 tacttctata tccaaaagtt ttgctattga cctcatatct tctctagtct ttgacctata
    19321 gcttgatatt aattcatctt ttattttctc tggttctaaa agatattctt catctcctct
    19381 tttgaaaaca acgtattttt gagcatatga acttctattg tagtatatat atcttgatat
    19441 ttttcttaaa atttgattat atgaaagtcg cttgttaata tcaagtttta agtactctga
    19501 aaaagcaaat agctcttttt ttgatttaaa taatgcttct ttctttatag ttcttgatat
    19561 tgaagttaca ctttcttgat atgcacactt ttttaacatt tttaaatata ctctattaag
    19621 accaatactt tgattgaatt tttttatatt attattaata atcttatcca agttaccttc
    19681 aaagtatttt tttgctcctt tttgtgatac ataactctca agttctttgt gggccatatc
    19741 aagtatcatt ttttttaaat cctctttata tcccctagat tccatgatac tccctccctt
    19801 agctatttat agtatattca aaaagctcaa gaaatgtgag tcattacaaa atttttttac
    19861 tttttatgta tatattaatt taaaagggga gataatagta atgtaaaatc aaacatactc
    19921 aatctccccc atttttagac ctctattact cccccaatag aggtcttttt ttagtttatc
    19981 aatttctatt caattccctt tagttcaaat cctaattctg ctatcacttc ttttaatttt
    20041 tcaatagata catcatcttt catatctaca gatgcacact tattttctaa atctacatct
    20101 aaaacttcaa taccatctat atcttctgtt aatgcagttt taagatggtt tacacaatga
    20161 ccacaactca taccttctac taatagtttc ttcttcattt aattacctcc ttatacccta
    20221 taagggtatt ctatcatata gtttatactt tttaaataga aattaatcat ttaatttgat
    20281 tcattcgatt ttatatctaa attagatatt ccctcatata catcaaaatc atttggagta
    20341 attttacctt ttaatctact attacttaca atatagttgt ttctcacagc taaacttata
    20401 aatgttgcat tatcttttat atatgtctgt acatctttgt atgctttttt tgtttcaatt
    20461 tcagacgtag aacttgtaag tgcagacata tatccattta acttatcatc agtatatcca
    20521 ctactcgcta taatactact tgcatctggt ataattgata gtttccatcc aactaatgct
    20581 aaatcataat ttttagaatt aagagcattt tccatatcct tatctgacag ctgatttact
    20641 gtactattta ttcctatagc ttttaagttt tcatttatta tgtttgcagt ttttattctc
    20701 tcactattat ctctgtttac tattatcttt aagttcattt cagaaagact tttctttact
    20761 tcctcttttt ctctattttt tttctcttca gcttcttttt tgattctatc ttcttctgct
    20821 tttttagctt ttgcttcttc ttctggcgta agcttctttg agttcttttt agctgatttt
    20881 ttctgatttt ttactttatt gtcattttga tttactgagt ttacatttgc atattctacc
    20941 ttagcaagac actctatcgc cttatcttta ttaaactcta aatccttcat ttcactatta
    21001 tagtattttg attttgaatt aagtggaaaa ttaactggag tagcatcatc catataacct
    21061 tcactaataa ttctatcttt atctatagag tgtgcaattg cttttctaaa attaacatct
    21121 ttgaaaaaag gattattata attaaataat acacactcat aatctcttcc ttgatatttt
    21181 gttatattaa actctttttc ttgaaactta cttaaatcat ttaatgtaac attggttata
    21241 tcactatcaa gagccataac catagatgtc cttgcatctt cattaggcac catacctact
    21301 tttatgttct tcatggtttt tggaacttct tcatagtaat ctttgtttac agataaaacc
    21361 attccttctc tttctgtata tgattctatt ttatatttcc catttcctat catattatta
    21421 ttcttgtcat ttacatcttt tgaatctaaa tgattttgag atacaattgg aaaaattaaa
    21481 ctatcaattg aaaaagcata tctagcttta aattttattg taaaatcttt atctgattta
    21541 atattcacag aagctatatt tttagtaaat tcattatatg gactatctgc acttttttga
    21601 attaaaccaa tagtatagtt tacatcattt gaagtaacat tttttccatc atgccacttt
    21661 gcatctttta gctttatatc tatactcatt ccatcctgag ctatattata ctccttaact
    21721 aattgaggaa ttacattgta gttctcatct atagtaaata aactatcata aactaaattc
    21781 attatataac caacagactt atcagtatta gttataggat ttatagtttt tggagttacc
    21841 attgttaagt ttatatactt agcatctatc gaactttgac tttgtaaact tgaatcacta
    21901 ctactttgtt ttgttttagt attactacaa ccagctacca taagagttat agctaaaacg
    21961 actgttaaga cttttattct cttcattagt tttttccttt cttgccatat atcttattat
    22021 aatgttcgta attatttttt actttttcta catattttga agtttgctta aaaggtatat
    22081 catgtagatt ttcgccatct ttactatatt cattattttc taaccatttt ttcacattac
    22141 ctgaaccacc attatatgca gctatcacta aatctaattt accaaattct ttgtataact
    22201 tacttaaata ccaacagcca agtcttatat tagtctctgg gtcgaaaata tccacatttt
    22261 ttaaatttag ttcctctgct ccccaatctc tagttatgtc taatatttgc attaatcctt
    22321 ttgcttcctt ttttgaaaca gcagaactat taaacttact ttcggcttta ataacactgt
    22381 aaactatatt ttcatctaaa ttaaattctt tcgaatactt ttctacatac tctgaatatt
    22441 ttttaggata taaaaattta tgtattactt tgctttccat taatagtgcc ccaaataaga
    22501 ttataaaaat agatagaatc aatactttct tactattcac gccaattctc cttcatgtac
    22561 tcaataaatt tatgtgcttt actttctaat tcagttattg ttcccgaatt atctattata
    22621 taatcaccat atttggattt ttcttcctga ctcatctgtg aatttatcct actaagtgct
    22681 tcctgctcac tgcaattatc tctcttttgt attctactaa tttgtacttc ttgtttacaa
    22741 gtaactacta atagcttgtc aaccatatct aaaaaaccac tttctaccaa tattgccgca
    22801 tctaacacta ctatattttc gccttttttt ctcaattttt ctatttcact tattattttt
    22861 tctcttattc gtggatgagt caagttattt aattccttaa gtttttcttc atcactaaaa
    22921 actattttcc caagagcttt tctatttaga gttccatcat cattttttat actttgccca
    22981 aaatggacaa atactttttc caataacaac ttatcctcaa atatcttcct ggatataata
    23041 tcagcatcca ctataggaat gttaagattt ctaaatatat ttgataggct gctttttcca
    23101 cagcctattc ctcctgtaag tcctaatatc aacataaaat cacctacttt gtctcatacc
    23161 aagaactacc tacatttaaa tctacatcta agtgaacatc catatttaca gcattttcca
    23221 tttcttcttt tactattttc ttaactatat ctatttcatc atcaattgct tcaactatca
    23281 actcgtcgtg aacttgtagt ataagttttg atttcaaatt gttctcttcc aattttttat
    23341 gaacatttat catagcaatt tttattatat ctgctgcact tccttgtatt ggtgcattca
    23401 ttgcaaatct ctttcctcta tttctttcca taaaattgct tgactttatt tctggtatat
    23461 atcttcttct gtttaaaatt gttacagcat atccatcctc actagccttt tctactgttg
    23521 tgtccataaa ctcttttatt ttatggtatt tagcaaaata actttcgata tattctttcg
    23581 cctttggaac aggtatattt aaatcatcag ctagaccaaa gtcactttta ccatatataa
    23641 taccaaagtt tacagcttta gctgcacctc tcaattcact agtaacatct tccataggaa
    23701 cattaaaaac ttgtgaagct gttttggtat gaatatcttc atgatgttta aatgcttcta
    23761 tcatattttc atcctgactc atatgtgcaa gaactcttag ctctacttgt gagtaatcgg
    23821 catcaaccaa cttacagtta tcatctgcaa taaacacttt tcttattttt ctacccattt
    23881 ccatttttat aggtatattt tgaagatttg gttctgtaga tgaaattctt cctgtagtcg
    23941 ttatagtttg attaaatgat gaatgtattc tattactttc tggatttatt atatttaaaa
    24001 gtccttcaac gtaagtagaa tttaacttta ctatttgtct atactcagtt atcttatcta
    24061 ttattggatg cttatctctt aatttttcta aaacatctgc attagttgaa tatccagttt
    24121 tagttttttt aattattgga agctctaatt tttcaaataa tataacaccc aattgttttg
    24181 gtgaatttat attgaatggc tctcctgata attcataaat ttccttttct aatgtagcta
    24241 ttatttcttt aaattcaaca ctcaattcat ccaatatact tttgtctact tttatacctt
    24301 catactccat atgacctaag acttctacta aaggcatctc tacatgataa aaaagaccat
    24361 ccatactcat atccattaga cttctttcca tcataggaac tgtttcttta actgtgcata
    24421 ttatttgacc catatactct gctaaatcct caaattctaa atcctcatag tttttagatt
    24481 taacaccctt tcctaataat tcttcttttg atttaacctt ttttgataaa tacttcatag
    24541 ctattgcact acaatcatat gaggtagatg aagttgaatc tattaagtac tcagctattg
    24601 ttatatcaaa atacatattt tctaagttta tattatatgg ttttaaagat atataatcat
    24661 cttttaaatt atatccatac tttttaattt cattatttga aaatatacta tctaactttg
    24721 acacttcatt tgaatctaca taatatattt tttctccatc tacacttata aaaatatata
    24781 ttatattttt ttcaagtata ttaccttgtc ctctaacagt ttttaaaata acttgtcctt
    24841 ttttgtttac ttcatctata aatttttcta tatcttttaa ttttaatatt tctatttttt
    24901 cttctacgtg ttctgtgtca tctgaatcga ccaaatcaac taatcttgat aagagacttg
    24961 taaatccaaa gtatctaaat ttatctaaaa gttcatcttt atcataatcg ccaaaaatca
    25021 tactatctaa atccacttct attggcacat ctcttataat tgttgcaagt cttttacttt
    25081 gaatagctat ttctttgttt tcctctattt tcttttttac actaccttta agcttatctg
    25141 tattctctat tagattctct atactagaaa actcttttat aagctttata cctgtttttt
    25201 cacctattcc aggtactcca ggtatattat cagacttgtc tcccataaga ccctttaaat
    25261 cgataaattg tgttggagtc atctcatatt tttcaattac agaattaaag ttgtattctt
    25321 ctacttctcc aacccctttt ttcgttataa gggtagttgt attatctgaa gcaagttgaa
    25381 tagcatcttt atctccagtt actatgtata ccttatatcc attttcttct gcttttttag
    25441 aaacagtacc tataatatca tctgcctcat aaccatctat ttctagtcta tttattctaa
    25501 atttatctaa aaggtctttt aatggctgta attgttctgc caattcatct ggcataccct
    25561 ttcttccagc cttataatct ttatattcta aatgcctaaa tgtaggtgct tttctgtcaa
    25621 aagctacact tatatgtgtt ggtttatata tatctatcat tttaaatagc attgtagtaa
    25681 acccatatat agcattggtc tttaaccctt ctttattatt catttctgga agagcataaa
    25741 aagctctatt tattattgaa tttccatcta ttataattaa ttttttttcc aaattatatc
    25801 actcctaaaa attatctatt tttgtaacat atatattata taccaatata gtgttattta
    25861 atattcccat aataataata gtatattaaa attaaatatg ttgcaaacaa tatatttttt
    25921 tgctataata atatacatga catgcccgag tggtggaatt ggtagacgca gtggactcaa
    25981 aatccaccgg actaatactc cgtgccggtt cgagtccggc ctcgggcacc attaaaaact
    26041 cctaaagcta tttattcaat agattaggag tttttttatt tttgatttaa attttaagtt
    26101 attttttgaa attactgtgt ctttttaaaa tttatataat tatataaatt tatcttctag
    26161 cattaattta aaaactaatt tacttttata taattttcta ttttatatca taacttatta
    26221 caacctttta tctaatttat tgtatacaac aacactacaa ataaattgaa ataaaataag
    26281 caatccaaga acaactaaaa cccacttgct tattaccatt acagaaaata cactcaatat
    26341 agaaacaata aatataagaa ttacaagaac atagcacatg atttgattag taatacgtcc
    26401 agctttatca cgtaacataa ttcttctttc atcattcatc tctatccttt gattttttat
    26461 cttttcttca taatctactg ctctttcagg tttactccaa tacatatatt gaagaatcat
    26521 acatacacct ggacctaaag cagctccaga taatccccaa ataaaagcct caaatgaaaa
    26581 ctcagtaaat gtagccaata aaacaagtaa aattccacaa ataaaatata taattccaac
    26641 atataacata ttctttttca tttttactcc tcctcataaa taaatatttc ttcaatactc
    26701 atatcaaagt atctagctat cttaaaggct aagatgatag aaggattata cctaccattt
    26761 tctaatgaac ctatagtttg tcttgatact tgcaaaatag aagcaagttc ttcttgtttt
    26821 atgcctcttt cttttcttat ttcttctaat ctatttttca catctatccc cctttaatat
    26881 ctcaactacg gaaagtctac tttccataat tatacaacta tattccattt atgtaaagct
    26941 tactttccat atttacaaaa aaataacaga ctattttcag cctgtaattt tagaatttca
    27001 tgttgtttat taattttaat gttatttatt aattccatat tatttattaa ttttaacttg
    27061 gtttctgaat tggaatccaa acttctgtag tataatcagg agcattgata tcgccattgg
    27121 gataaagttc aatatctggt aaattagcat acacataccc agaacttggt aaccactcag
    27181 taataattct tttttgcaat tcctgtattg ccagagcatt tggcattgga gaaacacatt
    27241 caaatactgc ccatgtgcct gatggtatta agtattcatg agtatcttct aatacaggct
    27301 tatctgttgg agcagcaata taataatcta aacccttact atttgtacac acactaactc
    27361 caagcaatcc acaaggttca ttatttatta actctgaaag tttatcaatt gttccatttt
    27421 ttatgcattt agcccacaac ttaggaactt tctcaaagca ctcttctaca atcatgttca
    27481 tactttcttt tataccaaca atacgaaacc ctttcttttc tacaatacga tattgcattt
    27541 ctatatttcc ttttacataa atgcttaatg tcatcttagg atatgctttt aaaacaacac
    27601 cctttgctct agctgcagat ggagaaatgt tatgtatatt ttgaaaagct ctattaaaag
    27661 atgttggtga atcgtaacca tacttctcag atagttctaa aattttgatg ttactgttct
    27721 gaagttcaaa tgcagcctta gtcatacgtc ttcgtctaat atattcagat aatggtactt
    27781 ctgcaatata tgaaaacatt ctttgaaaat gaaaggttga acaacatgca atttttgctg
    27841 cttctgcata atccactcca ctttcgagat tttcttcaat ataagtaata gcttgattta
    27901 atttatttat ccattccatt tgatattacc tccaagcaaa ttatacagtt taataaaata
    27961 atatacctct ctattcatgc acaaaattgt aagcatttaa atttataagc atttttcaat
    28021 caatacaata aatataaaaa ttgtattata caataaaaaa gttcaataac attatattgt
    28081 aaaatgttat tgaacttttt ataaaaatta atttaagtgt agttaaatta agtattacac
    28141 tatcttatat attacaatat cctactatct tatagtcttc tggcacttta taatctttgt
    28201 ctggagtacc taaattccat tttaaatcaa acatagttgc acttattatt gttgcaaaat
    28261 aaagcataac tataccaatg tctatttttt cttcatataa attagtatga ccatcttttc
    28321 taacagctaa aactacagtg cctccatcaa ctataaatct ccatggttgt ctatttaatg
    28381 tagatggagc taatcttgca tagtgaaatg catcaagaag tgctctttct tctaagattt
    28441 ctatacttgc aacatttccc cattcgttca tataaacaac atcttctaca cctagtctag
    28501 tagacccctt atctgttaca acttgcatat cagattttga ataattttgc ccagtttttg
    28561 tagcatttaa tactttagca ttacttacat tttctccata tccaatagct ataattcctg
    28621 ttacctcttt atctgaatta attccaagtt tttcttttac cttttttcca tcttcaaaag
    28681 taacccaaca agaaccaatt ccaagttctg ttactttaaa gattatactt tctcctatgt
    28741 accctgaatt ttcaatgtaa ttatctttta tgtcagataa tattataata taacttggag
    28801 aatctatcat atttcctttg taccctgcta ctttatctaa attattaaaa acttcacttc
    28861 tattcataat tcttacatca acatctattt ctggaactag tttcttacaa gattctgagt
    28921 atttcttaat ttcactaaga tattcttctt taatatctgt gttcttatat tctctaacag
    28981 actttctatt taatatgatt gttttataat ccataatttt atcctcccca aactagtatt
    29041 gtcttaatta tatacccatt aatatattat tttactcatt tatttaataa ataaagatta
    29101 caattttgta attattattt attaataata ttccactttt acattaataa tgaccagttg
    29161 tgcaaaatcc attgccaacc accaaataaa aacatattat aaaggtaata taaaataaca
    29221 aacaaatctt aacaaaaaat taaacttttt ttacacgaaa gaaggcttcc cgttgccttc
    29281 tttattttta taaaaaaata agctgtttct aaaatgattt cacatttttg aaacagccaa
    29341 gttattctat aatgacaact ataatttgag gatgtgaggg agagttgtca ttataaattt
    29401 agggtaatta tattatgttt taagctagtt ctttctatat ttatataata cattttatat
    29461 atgaatttta tatgagattg gtataaatga tatatgaaat tacatatgtt tatattatat
    29521 taaattttat tattacattt tccacaacaa tactcatatg tctccacatt atttggaata
    29581 tttacaaata caaattcttt acagttctca caatacttta atatagatat agttccacaa
    29641 gtgtaacatt caaatattac acaatcctca ttttcctcta ctatttcaaa ctttttacta
    29701 tgttcaccac actctgggca cttgtgatta tttacattca taaatactcc tccttattag
    29761 tatagttaca tgtcaaaaac ttaattaatg tattctatat tacttattat atattctata
    29821 ataaatcata ttctaatata ataaaacaaa ttaaataaaa aaaaaaccat aaattttaaa
    29881 tttatggatt aattcttgta atgataactc tatttgagga tgtgagggag ggttatcatt
    29941 acaatagggt attatattat ggtttttaag ctagtctttc tacacctcat atgatacata
    30001 tgctatatga attttatatg atttttacat aaatatttta tgaaatttaa attttttagt
    30061 cattttgaaa tttgtaaaaa gttctctaaa aatccacagc atcataaaca gccaaaattt
    30121 aagtaaaaca tcataaaaaa atttgtccaa taattttaac aagtaatctc acctaattat
    30181 aattattgac acaaacttaa ataaacaatc tataaatctt ctataaatct taaactttcc
    30241 ccctattgat acacaatttc tagtttgtca attttatttt gcatttctaa gaaaatttct
    30301 tcaacagaat tgttaaatat attgcctaaa tagattcttt tatctttttg aaatgaataa
    30361 gattttacac taccatcaga agaaatatat aaatgactcc actctgctcc atattttgaa
    30421 aacctctcct ttgtatcttt aaacctcata tcttctattt ttattgcttt ttctctgtct
    30481 cctcttacta cagtgtgacc cctttttatt aaactggtta tttttatatc ttcttttaat
    30541 ttaatacgtg tatcatcatt ttttgaatat actttccata tcactttatg acgataatta
    30601 tctaattcct ttattaagct tctatcaata gtaaaactat ctttagttac cacaattaaa
    30661 tcaaaatggt ctaacatata ttgaaatatt tctctcatac ttttattttt taaaatatcc
    30721 tcaccaataa cctctataaa tctacagcct ttctcataca tgcagttcaa taaacttgtg
    30781 atttcagaaa aataatcatt tgtattatta tctccatcta tttctatagt aaagtacaaa
    30841 ggagtaatgt atgatttatt tcctgttatc tcaaatacac ttgatagtgt ctgtatatca
    30901 tcttcaaacg ttaagtacac attctcataa aacataaaaa attccataac tttttctttc
    30961 atattatgga tatttctata tctattggat aatatattta ttatatcgtc agtagtattt
    31021 ttcccatcac attcagccag tataacaatt tgcttccaac ttaagctatc cacatttccg
    31081 atactttttt tatatccatc ttcttcgtca taaataggac caattataat gttttctttt
    31141 ggccacagat gaaccctagt tccatctttt aatttcggat atttattttt tacagactca
    31201 taaaatctac ttgtatcacc catgattctt tactaccccc catagtttaa atattctatg
    31261 ctaaaaatag cactttattt ttattaattc aacaattatt caaaattggt cattatatat
    31321 gtttaaatgg atttaacatc aaccttattt atatgttatt gatgtttata tatgttatat
    31381 aaaatcttat ttctcatact taaatgtaat cttaactatc tctggtttac aattaagcct
    31441 aattaaaact tttgagcctc ctaagccttt atttaaaatc atagtgctat tatctttttc
    31501 atattttccc cagtcatact taggaaagaa ctctctattt ggagataata cacctccaac
    31561 aaaaggtatt cttattatac ctccatgaac atgtcctgct aaaaccaaat ctgctcccca
    31621 ttttgcatat ttttcaaaga aaaatggtgt atgtgctaaa agaatgttat attcattttc
    31681 attaacttta cctagattgt cttctacaaa attgtcactt agttctaatt ttttgttatt
    31741 agcaaataaa tatggatagc attccaatgg tataattaaa ccatataaat taagatggct
    31801 attacctcta tatattctta cagattcatt ttccatattt attattttct ttgaatatag
    31861 ttccttaaaa taatccttat atagatgttt atactttttt attaaagatt tttgttcatg
    31921 atttccaatt atatgatata cttcatattt ttcaaccaaa ttatctatta aatctaatgc
    31981 aacttgaaaa tttttatttt ctccatctac taaatctcct gttataaata taacatctgg
    32041 gtttaatgat tcaattttat ctatcaatac cttattattt ttatcaaaca ccttattatg
    32101 taaatcactc acttgaacta ttttaaagtt atcaaattcc tttggaattt ttttgttgaa
    32161 aatagtatat tctgtcgttt ctatatgctg agtttcatac ttatacagaa tatacaataa
    32221 tattaatact aatactgtca ttgaaacctt tataatcata ttctcttcct ctttattcta
    32281 ttttgttaat attattataa tgcttttatg ttttttttaa aacaataaca tcatcacata
    32341 catataaatc ttttcgacat tataaaaata agttatcctt ttctaaaatt ttcatttatt
    32401 ttagaaaagg ataactataa aattatctat aaacctctat attgtgaaat atagtttttc
    32461 ctttatttaa atttttatat atatgctcta aagttccatc aaacctaata gaatgtccag
    32521 actctaaaat ataagttttc ccatccacat taaggtcaag tttaccttct attacgagta
    32581 catactcttc taaaacatga ctgtgtgtag atgaaacatg agtacagttt tcctcaagct
    32641 caattataaa aagttcaaac cttctttgag ggctaaaagg gtatattgga tataacttca
    32701 tgttcccttt ttgctcaaca ataggctcta cttcttcttt ctttataacc tcataggtat
    32761 ttgtattttc atttaatagt gtagtaaaat ttattttaaa tccagatgaa attttccata
    32821 aagtagatac tgtcggactt gattcacctc tctcaatctg tccaagcatt gctttactga
    32881 cacctgtcat ctttgaagct ttgtctaaac ttatgcccat ttcttgtctc aataacctta
    32941 aattttctcc cacattagga aatttattat ccatgttaaa atcctcctga tagctatttg
    33001 tattctatga tttttactcc tgtaagttct actatctctt ttaattttaa catatgattg
    33061 ttacaaattt catcaaattc atatatataa tcttcaagcc ctaatgcctt atatttatcg
    33121 attccaaatt tatgatatgg aagtaactcc atcttgcctc ctggaacatt ttgtttgaca
    33181 aataatgcag tatttcttat attttcttca ctatcattta ctccataaat aattggcact
    33241 cttataacca tagatttatt caactttgaa agcctacata tattatctaa tataatttta
    33301 ttacttactc ctgtatattc tttatgaata ttatcatcca tagattttat atctacaaat
    33361 atgtggtcta ttttctcaaa tacatcattt accttattcc aatcaaaata tccacaagtt
    33421 tcaattgcaa tatttatgcc tttatcataa aaaatatcaa ctaattctct taaaaaatca
    33481 atctgaagtg taggttcacc tccagaaaaa gttatccctc cattagattc aaagaaaaat
    33541 ataaaatctt tttcaacttc ctctactatt tcttcaacac tcatttcttc ggtcattata
    33601 caaatggcat ctttaagaca taccttgata cactctccac ataaatcaca tttattattt
    33661 atttgcgctc tatcaaatag tgaacttata ttttgaggac aaacatctat acatctatta
    33721 caggatacac atttatcttt cataacacct aattttacta tgttactcca ggaatctgga
    33781 ttagaacacc acttacattt taatttacat ccttctaaaa atatagtagt ccttattcca
    33841 tctccatcat ttactgaaaa atgttgtact tttacaactc tacctttttt actcataatc
    33901 tacctcataa ttaaaaattg ctatgtgaat ttctagctat tatagcatct tgcatttctc
    33961 ttgataaatt aacaaattga gtactatatc cagcaactct cactaataaa tctttatatt
    34021 cttctggatt ttcttgagct tttaatagag tatcatttga tatcgtatta aattgtacat
    34081 ggaatcctcc taaagcaaaa tatgatttaa tcatagatgt tagatttcta agtcctcttt
    34141 cagtttccac caaatcttga ttcagtctta aatttagaag tgttccacca gtatgaacat
    34201 catgatttat ttttgatgca gatttcattg ctgctagtgg acttgttgta tcagtaccaa
    34261 caaatggaga aactccctct gtcaaaggtt ttgtcgcttt tcttccacaa ggagttgcgc
    34321 ctacgatttt tccagttggt acatagtttg atatacccat aaatgcacta ttaaatttag
    34381 agccaaaaat atccttgtat tttctagttt cagtataata aaaatcagaa acttcaattg
    34441 ctaatgaatc tacataatca ttatcatttc catacttagg aacatccaac gcaagttttc
    34501 ttaattcttc ataaccttcc caatcattat ttagtgcttt tgttaattct tctaacgtag
    34561 tcaccttatc ctcaaatact aattttttta tagctgccaa tgaatttgaa actacaccta
    34621 accctatacc agttaataca ggacctatat tatattttgc tcctccttta cttaaatcaa
    34681 ctcccttatc caaacatcca tctacacaag tagataaaaa tggtcttggt accatttctt
    34741 tatgaatttg ttgagcaaca gtagttccta taacagaact ttttacaaga tatgctaatt
    34801 gctttaaaaa ctcatcctta agttcttcaa aagaagtaaa ttctgtgata ttttttgtat
    34861 ctaaccccat tttttctcca gttaatctac tcttaccttc attcatagca tattctaaag
    34921 ctgctgcaaa attgatattt acggctgaag tccactgtcc tgtttttcta aaatgaggaa
    34981 ctacacaacc acagttactc caatctctag catcttctgc atcatatcca tcttgtaata
    35041 acatttgtgc ccctgcacta tcgctgtgta tagcagggaa accagttcct tgttttacca
    35101 atttagcaac tgccattacg aaattgtctg gtgctccttg actcactcta acacttaatc
    35161 ctggttggtg agtttttaca ctttctgtag cctttaaaca catatatgag atatcattcg
    35221 ttccatcagt accatttctc tttttaccac caacagtaag attttggaat tgattatatc
    35281 ctgcaaagta atttgctgta tttgctgata tagtccatac ccattctgat aacttaatcc
    35341 aaagtgcttc tatcaattct tgagcttctg attcagttat tttaccttct ctagcatcat
    35401 tttcataata tggatacata tattgatcaa atctacctaa atttaatgcc aatggatttt
    35461 ctgatagaat accacctaat tgaacaaacc atacaaattg tattgcttca taaaaattag
    35521 ttggagggtt tgctggtaca ttcatacata tctctgaaat ttttataagt tctaactttc
    35581 tcttttcatc tgtttcttgc ttactcatct cttttgcaag atttgaatat ctttgtgcca
    35641 atgtcataat accctgtgca actatagaaa cagatttgta aaaatctatc ttttcaatat
    35701 tttctgaaac tgatatatct aattctttaa tttttttatc tgcatcttcc ttaatccctt
    35761 tatatccttt tacaaacaat atatcttgat aatctggtgt tacttcacca actgcctgtc
    35821 tccacttaga atcattatct attattcctg tatctactaa tatcttagct gtatcttctg
    35881 ggattcttgc taagaaaact tcttccaaag attttccctt ccaatatgga aatatttctt
    35941 ttcttataaa ttctatttgt tcttttgata ttacatatgg gtcttgagtt cttttatcaa
    36001 aagtatccat ttctttgtct acccattgcc aagaaaactc tggtgtcaat atccctgttc
    36061 ttctaaattt tcctgctgta cctactatta attcattttc aaagattttt ataggaagtt
    36121 ctttacatgt ctccaaaaaa gctttagctc ttcttataca gatagcttct ccctcagttt
    36181 ttttatgtga ttctgtaaaa attctagccc tttcacaaca tatcataggt ttagaattaa
    36241 tatattcttg tctaaatgct tctattctat tagtttgact catttaaaat ccccctttgg
    36301 ataatatata ctacattttt gtatgttata acatacttta attatattat actgtccttt
    36361 agcataaaat ggaagcaaaa taacttattt tttttcatct accttgaaca cctatatatt
    36421 aatacttttt ttgttaaatt tataaaatct aaaaaaattt tctatgatat attttctata
    36481 ttgttatagt tatgtctaat atactaatga tttgaaatat caaatttgta tactagactt
    36541 agttttaaaa ccttagttga ttgtttaact ttgttaactt caacatgtat atccttcata
    36601 taaggaagct ctacaacacc tactccctta ttaaagtctt cttcaaccat agaaaaaatt
    36661 tctttctcag ttaagttcgt atcagtcaat gatttaaata aattgtatat aaatttaaca
    36721 tatttatcac tctcagataa cttatcatct tctggatact ctttagcaca gagaatatct
    36781 attgttactt ttccttcttt atcgtcttca gacatagaat agaataaact tcgaatattt
    36841 ttttcaggtg gtttttcagc accatttata gatactacaa agctaccatc tgattttgag
    36901 ttagtaatac tataaccact gtctattata gctttcgaaa atctttgaga taaatctttt
    36961 tttacttctt ctctaccttt cgcatcatta atcttttgtg agtaattatt aaatagctca
    37021 atagattcat cttttttatc ttcaacttgt tctttcaatg aatctttact aatttgttct
    37081 gagctactta agttcttatc agatacagga gacatacacc ctaccatcaa tgctaagcta
    37141 agatttaaag ccacaattat tgccactcta tttatattcc tttttacttt catacacaat
    37201 cctcccatca cactaaccgc tctaaagtat tattcattgc actagaatat atttatattt
    37261 tctttatatt aaagatttat tattcaatac atatcacctt cctatccaat tcttataact
    37321 tactattagt aaaattataa gctccttaat acagaaataa ggatgttatc ttttgttatt
    37381 aaaagataac atccttattt ttttgtatca atacatatgt cccaactggt aacttaatcg
    37441 ccccaactgt cagtatcttg gtccaatggt atatatatat ttagtataaa tttatcgata
    37501 ctattttcaa ataataattc tccattatat ttttgtaaag aagatttaat actctgaata
    37561 cctattccat gtacgaactt atccattttg tttgttaaca atatattttt attaaaagta
    37621 agctgattta gtttactatt ttcacattta agtacaaaaa atgaccttgt tatagttcct
    37681 ttaattctaa tatatttatt aatattttca tccactattt tatcacatgc ttctattgca
    37741 ttatccaaaa tattagaaaa tatgcttgac acatctatag gttttacaaa atttactttt
    37801 gaaaaattta tagagcaagt aaatttaatt ccttttttta aacatacttc actcttttcg
    37861 ttgattataa tgtctaatat catatttcct gtattataga tatatttaaa tgtttttatt
    37921 tcatctttta aattgttaac atattcatta atttctttgc tattgtttct aaggttgtct
    37981 atacaataaa tgtgattatt tatatcgtga tatagtttct taactcgcat atgagattct
    38041 tgaatactta aataatgcgc atattgcata tctaacttat tttttattaa ttcagcttga
    38101 gattttaatc tattctcttt tattatttct ttaaacaata gtataagaaa tatactacta
    38161 gctcctacca attttggatt aacaaaatta tcaaaatagt atccttctga atgtaatgac
    38221 ccaaaccgtt ctaatttttt taatacataa tttacaagca tattcattgt attaactaat
    38281 aaggcaaata ataaatatac ataatttctc ttatcttggc taattaattt tatagacttt
    38341 ctaaaaaagc taaatataag atatataaga ataattatta aattactata tatctgaatt
    38401 tttatagatt ctaagtaagg tactaataaa gattctttag atgaaataac attacctgta
    38461 attgcaaaaa atagtattct atatataaca gcatccaaac atttataata tacactaaat
    38521 agataaaaga aaatggaata cttaatataa cccatcttat aaaaataatt atagtatcct
    38581 ccccaagcaa taaatgctaa tatataaaat aaatagttta tattactaaa gtaatttcct
    38641 ccattaaata atctacttat ctgaattata atataaataa gaaacaagac aatatagaat
    38701 actatttttt catttttttt actatccttt ttataaatta aaattccgtt cattatagaa
    38761 aactgtataa atatagataa aactactaat agctcagtca taaagtttag catattgtat
    38821 caccaagaaa ttttaagaat ttctccttaa cttctttata tctatatcta ctaataggta
    38881 cctcttctcc actttccagt attgcagtat taggctttat attttcaaca tatcttaaat
    38941 ttactataaa acttttatgg catcttataa atttatatgg atttaattct ttttctattt
    39001 tgcctaaact atattttata tcaaaattct tgtttattgt gtgtattagc atatcttttt
    39061 tttgaacttc tatatatttt atttcattta aataaatctt atatgtatta gacttatttt
    39121 ttattgtaat atgactttcc ttatttattt caatatcttt gatacaagtt agcacatgtt
    39181 tttttagttc ttccaactct attggcttta gtaaatacct ataagctctt acctcataac
    39241 cttcttgcac ataatctatt aatgaagtta caaatattat ttccatttca ttgtctactt
    39301 ctctaatctt tcttgcagta tccattccat ttaatttttc catttttata tcaagaagta
    39361 atatatctat atcttttaaa ttactttcca gtaactcttc tccagatata tacttttgta
    39421 tctcatattc tatacttata tcttttaata tattctcaag gtaatcttct attaaaagtt
    39481 gtgtttcttt ttcatcttca caaactgcta tcttaatcat aaaatgcctc cttacataat
    39541 ttctagttaa tacaattaaa atatttataa tatatttaaa gataattttg tatatctgat
    39601 actttaggat aacattttta aattattttt atcaatactc aatcccatac aactattatt
    39661 tgatatttca atttaaagac agaaattact ataattaaaa acagtactta ctgacaaaag
    39721 aagatgtctt aaaatattaa aaaaataatt ttaggacacc ttcttttatt tatttaaact
    39781 ttttaagcct taaataatac attaatctcc tatataattg tactgcatat aaaatcaaca
    39841 atataatctg tagtacaata gcagttacag atgctccata accactatat aaatttctta
    39901 tttcaatatt caataaatat ataaatatta ctactaacat aaagaatata aacatattca
    39961 taaataaaat tgactttact ttatttgttt tatcagaatc actatataat ttaggaggta
    40021 tgtctttgta ttctttcgac catatatacc atgcagatgt acccataaaa ctaccaatac
    40081 tcctaaatac aagattccag ccatttgatt tatgttcatc aaaataatta ttctctacat
    40141 catagtagta atcaacagtg tattttattt ttcttggact tcccttatga aaataaaata
    40201 ttcctccaaa tttatttatc ttatataaat tatatccttt agcttcaata tcttctaacc
    40261 atttctctat cttatcaggt gaactatacc agtcaaattt gaattttatt ttcttttcca
    40321 tagcactcat tctccaaaat gatattttta aatagcttaa aaatctattt aaataattat
    40381 atcattttga tactaatttt aggttacaat catatattta aaagttattt aaatagtaat
    40441 aaactaatag ccataacacc cattcctgca atcaatccat aaatagataa atgatgttcc
    40501 ccgtactctc tagctgatgg caataattca tctagtgata tgaataccat aattcctcct
    40561 acaattgcaa atacaattcc aagagttata tcatttaaaa aatttctaag aagtacatat
    40621 ccaataatcg ctcctaaagg ctcagacata ccagataaaa aggaataata aaaagctttc
    40681 tttttatctc ctgtggcata ataaactggt acagatacag atataccttc tggtatattg
    40741 tgtattgcaa ttgcaattgc tatcggaata gctattgtaa catcatcaag agctgatata
    40801 aatgtagcta atccttcagg aaaattgtga attgctatag ccattgctgt aaatatacct
    40861 gttcttaaaa gagacttatt ttttttgata ttcttttctt ctatattctc cattttcttt
    40921 atttcatgtg gattttcttc ttgtggaatt agcttatcaa taacagcaat gatagccata
    40981 cctataaaaa aagctattac agttgaccaa cttcctaatt tctctcccat agattttgtc
    41041 aaagcatctt gtgctttagg aaaaatttct atcatagaaa cataaatcat aacaccagca
    41101 gaaaatccta agcttataga aagaaactta gtatttgttt ttttagcaaa aaatgctata
    41161 caacttccta taccagtaga taaaccagcc aataatgtta tcaaaaatgc aaatattaca
    41221 ttattcaaat aaatcacctt ctttttaatt gatattgatt atcattttct ttatgctatc
    41281 atatatattt tatatagtca atatttataa aaaatataca caataaaaaa gcagagatat
    41341 aaacttagtc tctgcttttt ctttatagta tattaaaatt atttatttaa gaaatccact
    41401 gcatattgca catataaagc agttccaatt tctagagcat cttcatctat attaaagcat
    41461 ccatgatgat gaggataaca agcacctttt gactcatttc tagctcctac aaatgctagt
    41521 gcaccaggtg ctatattcat aaactcagct aaatcttctg ctcctgttac tttttcaaat
    41581 agtgttatac agtcttctcc aaatagctta attgctgcat ctgtagctat ctttgaacat
    41641 tctttgtcat ttataactgc tggtgttaag tacccatact ctaattcagc atctgctcta
    41701 tatgcttctg ctgtagattt tgctatacgt tctaaaattc taggaatttg ctttctaagc
    41761 tctggattaa atagccttat tgtaccttct aagatagctt cactcgcaat tacattaaat
    41821 ctagttccag aatttaaaac tccaacactc acaactaatg gttccagagg acttacttct
    41881 ctactaacca tagattgtag attcataact attgctgaac ttgcaagcac tgcatcaaca
    41941 ccttgatgag gaagtgaacc atgaccacct ctacctttta cagtaatttt aaagaaatca
    42001 gcagaagcca ttcttggtcc ttcttctacc gatatggtcc cagattctac atcagtccaa
    42061 agatgtattc caaacacact atctacacct tccatagcac catcttgaat catagctctt
    42121 gcacctttac caacttcttc tcctggctga aaaaataatt ttacagttcc attgatacta
    42181 tccttaatat catttaacac ttttgctgcc cctagaagca ttgatgtgtg accatcatga
    42241 ccacaagcat gcataagtcc ttcattttta gatttgtatt ctacatcagt gcattcaaca
    42301 acttgtagag catccatatc acccctaagt gctacagtct ttcctggatt agctcctttt
    42361 atagttgcaa ttacacctgt acctccagct gatacatatg gtatccctat tttatcaagt
    42421 tcttctttaa ctctctttga tgttctaact tcttccatac ttttctcagg attttcatga
    42481 aattcccttc taagcttaat tacatagtct ttataactct ttgttatttc tttaatatcc
    42541 attccatacc tccaagtagt acacattctt cttatttaaa atacttttta tcactcaaat
    42601 actatattag atttacaaag atacccgcta ttactacaga agctattgta actgtagtaa
    42661 atcctccaac aagcatcttt ggaagcatct tatccattaa aaattctttt tcttctggtg
    42721 tttcagccaa agcctttact gcctcttcag ttaatatata gtttggtgga aaaccataaa
    42781 gtgatgtaag cgaaactgaa aaagccattt ctttactttc acctaaaagc ttacctacta
    42841 taatagagaa tattcccata cctattacac caaatattat gattcctaca agaggaactg
    42901 ccatttcaac aagcattgct ggtgtagctt ttgctaaccc tgcaaatata aaagccatca
    42961 atgatgtcat taaccatcca aatgaaccag atttattaag tggttttcta tcaactattc
    43021 caagttctga tgctattaca ccaaatatta aacatataac atatcttgat acaacttcat
    43081 ttgtaagatt tgtaaattgt actgctaacc atgctacaaa tcctaatcta agtattatca
    43141 tatatgtcgt atcatatttt tctggtaagt ctggaattat cttaaatctg cttttttgtt
    43201 caggtaaatc cttaactttt tcttcatctg tatttacagt tacttttcca cttctaaagt
    43261 ctgataaaag tctttttcct tcttttttaa gcattaatgc tgtaatagga taacccgcaa
    43321 atccttgtgc aacatacatc aaaattgcaa gtactgataa acttgcaaga ccttttgctg
    43381 tagcagcatc tgccatcatt atagatgcaa ctagccctcc agtaagagga ggtgtagcta
    43441 ttactgctgt atcccatcca aatactactg tccctaatgc caaagcacct acacatattc
    43501 ctactattcc tgccaaggct attactattg ttttccattg acctgctaat tccttaatac
    43561 tcatcatagt tcccatatga gttattaaca aaagcatacc taattgagct aatggtgttc
    43621 ctaaagcggc taagtccaca atattttctg ggaaaaaagt ccaaaatcct actataaata
    43681 taattgctga tacaaaaacc gacggaacaa aagctttagt ttttgctgaa actatatctc
    43741 ctatcgtcag tgatactaaa actagacaaa aagctgttat tgcctccata caaaatcccc
    43801 cctcataata aatatatttt tataaaatgt atgtatagtt atccattatt ttactacttt
    43861 tgaaaaacat ttcaagtcaa aattcgattt ttttcataaa attcgataaa ttccataaat
    43921 taaagttagt tttctaaatt attctttttg taaaaacgtt atcccgacca ttggctattt
    43981 aactctattc ttacaaagag aattaaatgt aatttgcaaa aaataaaaca taggatacat
    44041 tttattcata aaacatctta taaatataaa atgaattgtc tttatgaatc ttaatatatc
    44101 ctatgtttgt aagtttaatg tgtatgttag ctatggtgta aaatttgtat actagttatt
    44161 gtgtaaccta ctatatactc catttttgtt tactagctct tcatgagtac ctctttcttc
    44221 aattccttta tctgtcaata ctatgatttc atcagcattt tttatagtag tcaatctatg
    44281 tgcaacaact aaatttgttc tatctttact taattcctct aaagactctt gaatttctct
    44341 ttctgttaca ttgtctaaag cagatgtagc ctcatccaat attattattg gaggattctt
    44401 taaaaagatt cttgaaattg aaattctttg tttttgccca cctgataatt ttacgcctct
    44461 ttctcctata taagtatcat atccatctgg caaagtctct acaaagtcat gtattctagc
    44521 ttttttagca gcagctatca tttcttcatc tgtagcatta gggttcccac aaagaatatt
    44581 atctcttata gttcctgtaa ataaaaatac atcttgttga actataccta tattttttct
    44641 caatgatttt aaactaacat cttttatact ctttccatca atttttatat cacccttatc
    44701 aaattcataa aatcttggca atagattaca taatgtagtc tttcctcctc cagaaggtcc
    44761 aactaatgct atagtcttac cagcttttat agataaactt aaattatcca aaacttgagt
    44821 cttatcttca tatgtgaaag atatattttc tatctcaatg tcacctttta cattttcaag
    44881 ttcaatagga tttttagctt ctttttgatg gtcttgttcc attatctcta tgaatctttt
    44941 aaaacctgtc ataccatttt gatattgctc tgtaaagttt attaattttt tcataggttg
    45001 tataaacatt tttacatata gtatataagc agcaaaatca cccaaagtaa tctttcctat
    45061 ataagtaaaa taaccaccag ctattacaac tacccactct aagatatcca tgtataaatt
    45121 cataccagag taatattctg ccattacctt ataagaatct tgtcttgctc ttctaaataa
    45181 tttattgctt cttacaaact tatttaactc ttctttctca gtacaaaaag acttagttac
    45241 cttcatacct gctatactat tttctaaagt agcatttaca tcacctatag taactctact
    45301 cttcataaaa gcaatattca tcttatcttt tttagctatt gcaaaccaaa ttataaacgg
    45361 taatatagca aatattataa gcgtaagagg aatatttata tctataagta taaagaatga
    45421 accaaccaac attactatag aaatgaataa atcttcaggc ccatgatgtg caagttctgt
    45481 tatatccatt aaatcattta ctattctaga catagttact ccagatttgt tattatcaaa
    45541 atacgtattt ggaagtcttt gtaagtgtgt aaatacctca tttctcatat cagcttgcat
    45601 atctacacca ataacatgtc cccaatactg cataaaatag ttaagccctg cttttgctat
    45661 aaatataagt ataagtgcta ctgcaaaaac tactaacatt cttaagtttt tatttggaac
    45721 aacgtcatcc attatatttc tagttatcat aggataaact aaatcacata aggaaaatag
    45781 aaaagctgct ataaggtcta gtgtaaaaat tttcttatat ggcttataat atttgacaaa
    45841 ttgttttatc ataagtatta aatccctcct ttattagtac ttctattagt acttctaaaa
    45901 aatacccata aattagtata acataacaat aactttaaat cttttataaa cttacaaagt
    45961 aataacaatc tttttcataa aattgaagat ttatatcata tagttgtata cttgaaacaa
    46021 cataaaaaga gctatatctc tatatttaaa attttataaa gatacaactc tatatttaat
    46081 actaaatttt ttttataaat acaaaactac ttcaatgcat ttaatatctt tttatctaat
    46141 gctgttgtat ttatacctgt tatatatcct gttgccttta ttgtaggtat atcataagtt
    46201 gttggtagta tagtagtaga tacgattaaa tcagcacctt cttgttttgt tgaacattca
    46261 gcaattttac attgctcaac ttctgcttgt atattatttt ctttaaccaa tctttctact
    46321 ctgtcacaaa ctattgttga tgttgcgatt cctgctccac aggctactaa tatttttttc
    46381 atatattgtc ctcctacaat ttctttaata ttttataact caaaataaaa ttgataaatt
    46441 tcttatatct ttatttatat aa
//
LOCUS       DAEOSP010000007        46120 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.007, whole genome shotgun sequence.
ACCESSION   DAEOSP010000007 DAEOSP010000000
VERSION     DAEOSP010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46120
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.007"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            164..3712
                     /locus_tag="KRH54_000300"
     CDS             164..3712
                     /locus_tag="KRH54_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG8577979.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADIALY
                     SAKGKGKNCYTLYDKKFDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            3897..5831
                     /locus_tag="KRH54_000301"
     CDS             3897..5831
                     /locus_tag="KRH54_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBG8577980.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5937..6932
                     /locus_tag="KRH54_000302"
     CDS             5937..6932
                     /locus_tag="KRH54_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG8577981.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6960..7688
                     /locus_tag="KRH54_000303"
     CDS             6960..7688
                     /locus_tag="KRH54_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG8577982.1"
                     /translation="MEVTLSCVILLFLWQIIALKIDNDIFLPTVGQVFNSIKEIMLNS
                     SFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRNLLKPINALTRSIPTMILVV
                     LALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNLLEMANVYKIRFIDKVLKIY
                     LPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTMIQTEKINFNTSGIFAWIII
                     VLLISAILQIAQKFLARRAFLWKR"
     gene            7676..8374
                     /locus_tag="KRH54_000304"
     CDS             7676..8374
                     /locus_tag="KRH54_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8577983.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8497..8970
                     /gene="ybaK"
                     /locus_tag="KRH54_000305"
     CDS             8497..8970
                     /gene="ybaK"
                     /locus_tag="KRH54_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBG8577984.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9167..10006
                     /locus_tag="KRH54_000306"
     CDS             9167..10006
                     /locus_tag="KRH54_000306"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBG8577985.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10019..10567
                     /locus_tag="KRH54_000307"
     CDS             10019..10567
                     /locus_tag="KRH54_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBG8577986.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10633..11814
                     /locus_tag="KRH54_000308"
     CDS             10633..11814
                     /locus_tag="KRH54_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBG8577987.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12109..12495
                     /locus_tag="KRH54_000309"
     CDS             12109..12495
                     /locus_tag="KRH54_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8577988.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYDMDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEEFYEKNGCQRSLQSYIIKKKDSY"
     gene            12645..12908
                     /locus_tag="KRH54_000310"
     CDS             12645..12908
                     /locus_tag="KRH54_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG8577989.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13037..13879)
                     /locus_tag="KRH54_000311"
     CDS             complement(13037..13879)
                     /locus_tag="KRH54_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG8577990.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14174..14908
                     /locus_tag="KRH54_000312"
     CDS             14174..14908
                     /locus_tag="KRH54_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8577991.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            14918..16051
                     /gene="nagA"
                     /locus_tag="KRH54_000313"
     CDS             14918..16051
                     /gene="nagA"
                     /locus_tag="KRH54_000313"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG8577992.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            16150..16899
                     /gene="nagB"
                     /locus_tag="KRH54_000314"
     CDS             16150..16899
                     /gene="nagB"
                     /locus_tag="KRH54_000314"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBG8577993.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            17074..17346
                     /locus_tag="KRH54_000315"
     CDS             17074..17346
                     /locus_tag="KRH54_000315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8577994.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            18006..18710
                     /locus_tag="KRH54_000316"
     CDS             18006..18710
                     /locus_tag="KRH54_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8577995.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            18712..20199
                     /locus_tag="KRH54_000317"
     CDS             18712..20199
                     /locus_tag="KRH54_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8577996.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            20404..22017
                     /locus_tag="KRH54_000318"
     CDS             20404..22017
                     /locus_tag="KRH54_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG8577997.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(22380..23210)
                     /locus_tag="KRH54_000319"
     CDS             complement(22380..23210)
                     /locus_tag="KRH54_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8577998.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKSACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            23283..25010
                     /locus_tag="KRH54_000320"
     CDS             23283..25010
                     /locus_tag="KRH54_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8577999.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FIYDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            25047..26909
                     /locus_tag="KRH54_000321"
     CDS             25047..26909
                     /locus_tag="KRH54_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8578000.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            27242..27931
                     /locus_tag="KRH54_000322"
     CDS             27242..27931
                     /locus_tag="KRH54_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBG8578001.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            28027..28713
                     /locus_tag="KRH54_000323"
     CDS             28027..28713
                     /locus_tag="KRH54_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBG8578002.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            28730..30637
                     /locus_tag="KRH54_000324"
     CDS             28730..30637
                     /locus_tag="KRH54_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBG8578003.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKHSGNQSDEKEVNAELTSSVVKA
                     NTDNENKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            31089..31778
                     /locus_tag="KRH54_000325"
     CDS             31089..31778
                     /locus_tag="KRH54_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578004.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADN
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            32214..32753
                     /locus_tag="KRH54_000326"
     CDS             32214..32753
                     /locus_tag="KRH54_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8578005.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            32772..33815
                     /locus_tag="KRH54_000327"
     CDS             32772..33815
                     /locus_tag="KRH54_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8578006.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            33817..34653
                     /locus_tag="KRH54_000328"
     CDS             33817..34653
                     /locus_tag="KRH54_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8578007.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            34758..35432
                     /locus_tag="KRH54_000329"
     CDS             34758..35432
                     /locus_tag="KRH54_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8578008.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            35432..36484
                     /locus_tag="KRH54_000330"
     CDS             35432..36484
                     /locus_tag="KRH54_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG8578009.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            36714..37562
                     /locus_tag="KRH54_000331"
     CDS             36714..37562
                     /locus_tag="KRH54_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG8578010.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            37552..39216
                     /locus_tag="KRH54_000332"
     CDS             37552..39216
                     /locus_tag="KRH54_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG8578011.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYIGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     LTKSDTRFKSISNISKVLIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            39265..40521
                     /locus_tag="KRH54_000333"
     CDS             39265..40521
                     /locus_tag="KRH54_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG8578012.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            40544..42625
                     /locus_tag="KRH54_000334"
     CDS             40544..42625
                     /locus_tag="KRH54_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBG8578013.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIIVMIL
                     SMLYIKKYKRR"
     gene            42662..43747
                     /locus_tag="KRH54_000335"
     CDS             42662..43747
                     /locus_tag="KRH54_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG8578014.1"
                     /translation="MRKYMNEFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            43801..44925
                     /gene="wecB"
                     /locus_tag="KRH54_000336"
     CDS             43801..44925
                     /gene="wecB"
                     /locus_tag="KRH54_000336"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBG8578015.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            45003..45977
                     /locus_tag="KRH54_000337"
     CDS             45003..45977
                     /locus_tag="KRH54_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG8578016.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNGKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT        18394 a         4673 c         7994 g        15059 t
ORIGIN      
        1 aataaagatt ttacaaataa atcttaagct atcttattta attctaatac aggaagaagg
       61 taaatgtata ttgaaaaaat catagaaata gaagtaaaca atttaaataa atacaaaggt
      121 ttgcaaaaga agtaatataa ttatatacaa tgaggatgtg agtatgatga acagactaaa
      181 gccaagaatt ttaggtatta gtatcttgtt tattattata attattttaa catgtggagc
      241 tgttttcaag tttaaattta aattagataa taaattaaaa gaaaccacac atacatattt
      301 gtctgagttt acagaacaca atataattaa tctcaagaca agacttagtg gtcaatttga
      361 catgcttgaa tctattgcta acttcatagg aaacttgaat aatataaatg atgaaatggt
      421 gattgattta atggattctg atgtaaagcg aagttcttta attagaatga ctatctctac
      481 attagatggg gaatgttatt ctaatgatgg aattacccat aatgttcaag atagagatta
      541 ttttaaaaaa gctaaaaatg gagaaaagaa tatatcagag cctttaaaat ctattgttaa
      601 taataaagaa ataatagttt tatctacccc agtttataaa agtgggaaaa tagttggtgt
      661 tttaagtgga atttatgatt caaaaaagtt aaatgagtta ttaggattat ctgcatttga
      721 tgaaaaagca gatgtttttg ttgcaaagaa aaatggagat atagttgctc aaaaaacaaa
      781 taaaaataga aagacgctat catgtatatt tcagcttttt gacaaagata attgtgatga
      841 agatgattgt ctaaaaacta taaaagaaaa tatgaaaaat gataagtctg gatattttaa
      901 ttataactta gataatgaaa aatttatgtt aaactatcag ccacttggag ttaatgattg
      961 gtatgttttt tctgttgtgc caaataaaat agtatctatt cagtcagaga atatatctta
     1021 tgatgtatat atttttgcat tagaaatact actagttata ttgattttta taatatatat
     1081 agcatgtatt ataaataata acaccaaaat tattataaat gaaaaagaaa aatttaaagt
     1141 actcactgat aatatattag gaggggttaa aacctgttta aaagatgaat atatgactat
     1201 gacatatgta agtcaggggt ttttagagct tacaggttac tcttgtgaag atttaaataa
     1261 aatatttaaa aatagattta aagacatgat ttataaagat gatttagaca agtttaattg
     1321 tattattcta aatcaattag aaaaacaaga taaaattgaa gttgaatata gaattgtaag
     1381 aaaagatgga agtataattt ggattttgga gagaagtaag tctataaatg ataataaagg
     1441 aagtgagtgt attcagggag ttttaactga tataacagaa cttaagaaaa tacaagaaga
     1501 attagattca aaaagatttg aaatacaggc tatcaatgat agtattgttg gtggaatagt
     1561 gataactgag ataaatgatg attttgatag tatatattta aatgaaggtt atttaaatat
     1621 gattggatat acacgcaaac aactggaaga tgagctaaat aataaattaa tcaaacttat
     1681 ttttaaagat gatagaacag aattaattaa tagtataaac aaacaactca aattaagtga
     1741 tacatttgca tctgaacaaa gagttgaaaa aagggatggc actattatat ggattttatt
     1801 aaaaggaaga aagatgattt atgataatgg aaaatctgta ctttgttgtg ttattgtaga
     1861 tattacttct gctaaattat tagagcaaga acttaaaaag agtgaagaac gtcatgaaat
     1921 cattctaaag cagacgcaaa atattataat agaatgggat atagaaaaag ataaaatgaa
     1981 tatatcacct aattggtata ataagtttaa atatgaccct ataactgaaa acgcaacaaa
     2041 aaatataatt aaatcagtag atatatatca tgatgatatg gataagttta tttcattaat
     2101 taaacttact tcacaaggta gtgagtatga agagtgtgaa attagactta gaactagaga
     2161 tactatttac gcttggttta gtataaaggc aacaacttta tttgataata atggtaaacc
     2221 ttataaagta gtaggaagtt tgactgatat tgatgaatca aaacgagaag ctgaaaagtt
     2281 gaaagaaagg gcagaaaaag accctcttac agatctgtat aataaaaaag catcacaatc
     2341 acttatagag gagtacataa cattggataa aaaaagaaaa ggtgctttaa tgattattga
     2401 tattgatgac tttaaaggaa taaatgataa tttaggacat ttatatgggg atgctgtatt
     2461 aagtgaaatt gcaaatgacc taataaatct atttagagca accgatatag taggcagaat
     2521 tggtggagat gagtttattg tatttataaa agatatttct gaaacaagag atattataaa
     2581 taaagcagaa gaattggtaa aaacatttga aaggtctttt ttaggtaaag gacgaaatta
     2641 taaaatatca ttaagtgtag gaattgctaa gtttccacaa gatggtatgg actatatgac
     2701 actatttaga aatgcggata tagctcttta ctcagccaaa gggaagggca agaattgtta
     2761 tacattgtat gataaaaaat ttgataaagt ccaatatata actaatacaa gaaatgcagg
     2821 agatagtgat aatgtagaga tgtcttttaa agaacatatc acaaattata tatttgatat
     2881 actatatgaa tcaagtgata tagaagtggc agtaaattta attctaagta ttgtaggtaa
     2941 acactttaat gtgagtcgca tgtatatatt tgaaaattca gatgataatc gagtatgtaa
     3001 aaatacattt gaatggtgta acgaaggtgt aacctcggaa attaataatc ttaaaaatat
     3061 ttgttacaag gatttaggca actatctttt aaattttaat gcagatgggt tattttattg
     3121 taatgatatt gaaaatttag gtagtgatgt gaaaaaagta cttaagccac aaggcatacg
     3181 ttcaatatta caatgtaaaa ttttagaggg taatgaattt agagggttca taggatttga
     3241 tgaatgttca tataatcgta tgtggactaa tgatgaagtg aacactttga catttatatc
     3301 taaaacgctc agtatgtttt tattaaaaat gcgtattcag agtaagctag aagagtcttt
     3361 aacaattcaa aaatctgtga tggataatat ggatatatgg gcatatgttg ttgacaggaa
     3421 tacctatgaa ttactattta tcaataaaaa aatgcttgag tcagcaccaa acactcatgt
     3481 aggtgattta tgttataaag cgatatggaa taatcaagat aaaccttgta aaaaatgccc
     3541 tatgatatat ttaaaagatg gtgatgatac ttataaatca ttcatgcata ataaaaagtt
     3601 tgaggtacag gcaaatataa ctacaactac tttgaaatgg tttgatggta aagatgcatg
     3661 tttaatattt ggaagtgaaa taactagtat tgaagctgaa gaaaatactt aaaatactgt
     3721 gttatatata tacttaaaat cgcaatttga atttattaga cttaaaatgt ctatataaat
     3781 catttaagtt gtggttttaa gtatttttta tgatataatt aagcgttaag aacaaaatta
     3841 aaagatactt ttgtataaaa aaatatttgt taaatatata ttgagcaggt gataatatgg
     3901 aaaataagtt tttaccaata agcaaacaag atatgataga tagaggatgg gaagaattag
     3961 attttgttct agttacagga gatgcttatg tggaccatca tagttttgga acagcaataa
     4021 tatctagagt tttagaaagt gctgggtata aagttggaat aatagctcaa ccagactgga
     4081 gaactacaga tgattttatg aagctaggaa aacctagact tgcatttcta gttaatgcag
     4141 gaaatatgga ttcaatggtc aatcattatt ctgtaagtaa aaagcataga gaaaaggata
     4201 tgtattctcc aggtgggaaa atgggttata gaccagatag agctactata gtatattgca
     4261 ataaaataag agaagcatac agtgatgttg caatagtaat aggtggaatt gaagcgagct
     4321 taagaagatt tgcacactat gattattgga gtgataaggt tagaaagtct atgttaatag
     4381 atagtggagc agatttatta gtttatggta tgagtgaaaa gcagattgta gaagtagcca
     4441 atgcacttaa tgatggatat gacccaaaat acattagaca tattgatggg acatgctata
     4501 tttcagatac attggaagaa atatatgata aatatatatt aattccatca tataaagaaa
     4561 tttgtgaaga taaaatgaag tatgtagaag cttttaaaat tcaatatgat gagcaagacc
     4621 catttagagg taatataatt gttcaacctc atggaagtaa gtatctggtt caaaataagc
     4681 cagaaaaacc acttagtaga gaagaactag atgaagtata tggattacca tatcagaaga
     4741 cataccatcc tgtgtatgaa aaatttggag gaataccagc tatagaagaa gtaaaattta
     4801 gtatagtaag ttcaagaggc tgttttggta gttgttcatt ttgtgcaata acatttcatc
     4861 aaggtagagc agtgcaaagt agaagtgaaa aatcaattat tgatgaagca ataggaataa
     4921 caaatttaga tgattttaaa gggtatatac atgatgttgg aggtccaaca gctaatttta
     4981 gaagaccagc atgtaaaaaa caaattacta aaggtgcttg taaaaataga caatgcttat
     5041 ctccatcacc atgtaaaaat ttagatgcag accacagtga atacttacat ttacttagag
     5101 ctgtaagaaa attacctaaa ataaaaaagg tgtttgtgcg ttctggaatt agatacgatt
     5161 atgtaatggc agataagaac aataaatttc ttagagagtt gatagagcat catgtaagtg
     5221 gtcagttaaa ggtagcccca gaacatatat cagaagaagt tttaaagtat atgcaaaaac
     5281 cagcaggaaa aacttatgat aaatttagac agaagttttt tgctataaat gaagaattag
     5341 gtaaaaagca atatttgata ccctatttaa tgtctagtca ccctggttct accttaaata
     5401 gcgctataga gttagcagag tatttaagag atactcacta ccaaccagag caggttcagg
     5461 atttttatcc aactccaggg actctatcta caaccatgtt ttatacaggt attgacccgc
     5521 ttacaatgaa gccagtttat gttcctaaat caaaacgtga caaggcgatg caaagggctt
     5581 tattacaata tagagcacct agaaattatg atttggtata ctctgcgtta gttgaagcag
     5641 gaagagaaga cttaattggt tttggacata gatgtcttat aaagccaaaa aatgaaaagc
     5701 cttattttaa tagaaataat tctaaaaaaa atgtatctaa aggaacaaat aaaaacaaaa
     5761 aaactaatac aaataacaga agtaataaaa atcaacaaaa aagttcaact aaaaaaaaga
     5821 aaaaacatta aagtataaat aaaaatactt aaaaaagaag gattattcga taaattaaca
     5881 gaaattagta taaataaata taattatttt aatgacgtta cctttgggag gaagagatga
     5941 aaaagtatat ttctataata ttattagtgg tgttgactat ggttgttttg gtaggatgtt
     6001 caccaggaaa agataaccct aaaaataagg agctatcagt tgtcaaacaa gtaaaagtag
     6061 ctgttccaga tggactacca gcagtggcaa tagctaaact tgcaaatgaa aatccagaga
     6121 ttaaagaagg atatgaaaca gtgtatagca ttgaaaagac acctgaagcg atttccacta
     6181 gagtgatgaa aaaagatgca gatatagcta tagttccttc aaacatggct gcaatagcgt
     6241 ataataaaac ttcatcatat aatattgttg gaactgtagg aatgggttca ctttatttgg
     6301 tgtcaacaga aaatataaaa gattattctg atttagttgg taaagaagtt ggatgtactg
     6361 gaaaaggact tactccagac ataactataa aaagtctttt gttacaaaag gatataaatt
     6421 attcagatat aaaatttaat tatgtaaatt cagcaagtga actggtacca ttattggcta
     6481 ctggtaaagt taaaacaggt attgttccag aacctgcact tagtgctttg atgtctaaaa
     6541 atccagacat aaaaattata aaaagtttaa atgactcatg gaaggaagta agtggttcaa
     6601 aagatggtta tcctcaatct actttaattg taaagtctga atttttaaga gataataaag
     6661 attttgttga ttcttttgta ggtcaattat caaatagtat agattgggca aacaaaaatc
     6721 ctgaagaatt aggtgcatat tctgaaaaaa taaaaatatc tactgaatca aagataatag
     6781 gaaaatctct tgaaagagct aatttaaaat atattccagt taaggacatg attaaagatt
     6841 ataaaaatta ctatgagaaa ttagctaatt ttgatgataa gacattagga ggcaaagtac
     6901 cagatgaagc aatatacttt gttgagaaat aaaaaaataa agaacaattt atagataaaa
     6961 tggaggtaac actttcttgt gttatcctct tatttttatg gcaaattatt gctttaaaaa
     7021 ttgataatga tattttttta ccaacagtag gtcaagtatt taatagtata aaagagatta
     7081 tgctaaattc tagtttctat atagatatac tttactctat ggggagatgt ttttttagtt
     7141 ttttactggc tatgattttt gctattatac tttcaatagt gtcttattta aatagatttt
     7201 ttagaaactt attaaagcca atcaatgctt tgacacgttc aataccaact atgatattgg
     7261 tagtacttgc acttatttgg tttgaaaagg atagtacccc ttttatagtt ggatttacta
     7321 tagtttttcc tatattgtat gataacgttt tgggcgctat attgaatata gacaagaact
     7381 tattagaaat ggctaatgtg tataaaataa ggtttataga taaggtgtta aaaatttatt
     7441 taccagccat aaaatttcaa attatatcta tactagtatc aacattctct ttaagtttga
     7501 aagttgctat agcaggagag gtatatagtc aacctacata tggtattgga actatgatac
     7561 agactgaaaa aattaacttt aacacatctg gaatatttgc atggataatt attgttcttt
     7621 taatatctgc tattttacaa atagctcaaa agtttttagc aaggagagct tttttatgga
     7681 aaagataaag ttgagtattg aaaatataaa taaaagatat gatagtcgta ttatatttag
     7741 agattttaat attgattttt atgtaaatga ggtaaattgt atccttggaa aatctggttg
     7801 tggaaaaact acactcttga acataataag tgggattatt aaaaatgaca ccaacaatct
     7861 aaatatcaaa gaaaatttaa atagagtagg aaataagctt gaagctagtt acatatttca
     7921 agatgataga cttatagact ggttaacagt agaagaaaat ataaagatag ttgtaaataa
     7981 atactataat aaaactcaat taaataaaat ttgtgatgaa tacttggaat tggttggaat
     8041 tagtgattat aaaaaattct atcctcagat gcttagtgga ggtattagac aaagagtaaa
     8101 tattgctaga gcttttatat atccatcaaa aaatattata atggatgaac cttttaaatc
     8161 tattgatgca aagaatacac agcttataat ggataatttc agaaatatat taagaaaaga
     8221 aaaaagaact gtgttatttg taactcataa tattgaggaa gcattgtttt tagcagatag
     8281 gatatttatt ttaggagact ctccaattag aataaaaaaa atattaaaaa attctaagga
     8341 attggaaaaa aatgaagttt tgaagttgat atagagttat aataatactg tcgttttaaa
     8401 atatatttgg ttaaaatcta taagactata ttaaaatagt acagaatatt caaaactgtg
     8461 tataatgttc gtattttata aaaaggaagt gaatgtatgg ttaagactaa tgctatgaga
     8521 atattagatt caaataagat tgattataaa gtaatgtctt atgaagtgaa aagtgaacat
     8581 gtggatggtg ttgaggtggc acatgatata ggaagagatg taaatgaagt gtataagaca
     8641 ttagtaactc aaggagttag taagaatata tatgtatatg taataccagt acatgaaaac
     8701 ttagacttaa aaaaggctgc aaaagtagct aaagagaaaa gtgtagagat gattcatgta
     8761 aaagatataa ataagcttac aggttatatt agaggcggat gttctcctat aggtatgaaa
     8821 aagctttata aaacttttgt aaatgaaagt gcaaagaatt tagatacaat tattgtgagt
     8881 gcaggaaaaa taggatatca aatagaacta tctccatttg atttacaaag acttataaaa
     8941 gtagaatttg tagatgtaat aaaaaaataa gaattacata taaaatatag agcttatatt
     9001 cttaataata ggtgttgtag tattaataaa atgaataata attttaaaat aaatattcag
     9061 ttacttatgt gtaattggta taaaaatatt gagaaatttt aaaaacaatg gtaaagtgta
     9121 agtataatca cgaatatttt ttataaagta atggagggat gttcgcttga gaaaaataaa
     9181 atctgaacaa atcgtcgagc aggtaaaaaa actttgtata gaagcgagcc tatatttagg
     9241 agaagatgtt ttaagctgta taaaagaaaa agcgaaaagt gaaaagagtg aagttggaaa
     9301 aaatatattg aatatattag ttgaaaatgc tgaaatagct aaagaaaaaa atataccaat
     9361 atgccaagat actggtatgg ctgtattttt tgttgaaata ggacaagaag ttttaattga
     9421 aggagacaca cttactgatg ctataaatga gggagtaaga caagggtatg aagaaggcta
     9481 cttaagaaaa tctgttgtaa gtccaattaa cagagtaaat acaaaagata atacacctgc
     9541 tgttattcat tatgatatgg taaaaggtga taaaattaaa atagaatttg cagctaaggg
     9601 atttggaagc gaaaatatga gtaagatgaa aatgttaaag ccatcagatg gattggaagg
     9661 aattaaaaaa ttcataatag atacagtttc agaagctgga cctaacccat gccctcctat
     9721 ggtaattgga gttggtatag gtgggactgt ggacaaatgt gctcaaatag ctaaaaaggc
     9781 actttttaga gaactaggtg aatttaataa ggacgaaaat atagcaaaac ttgaaagtga
     9841 actattaaca gctattaata aacttggtat aggacctcaa ggcttaggtg gtactacaac
     9901 agctttggga ttaaacatag agacttttcc tacacacata gcaggtttac ctgttgttgt
     9961 aaatataaac tgccatgcat caagacataa gaaggtagta atttaggagg aaaataaaat
    10021 gataaaaata actacacctg taaatgaaat tgatatagca aaattaaatt gtggagatac
    10081 aattagtttg agtggtatac tttatacagc aagagatgct gctcataaaa gattgataga
    10141 ttgtataaat aaaggtgaag aattaccatt tgacgtatat ggtcaaggaa tatattatgt
    10201 aggacctact cctacaaagc caggagaagt tataggtgct gcaggtccaa cgacgagtta
    10261 tagaatggat gatttgacta tacctctttt agaaagaggt cttagattga tgataggcaa
    10321 gggaaaaaga agtgatgaag ttatagaagg tatgcaaaaa tacggagcag tttatcttgc
    10381 tgcaatagga ggagctggag catatatttc aaattcaata aaatcatgtg aaattattgc
    10441 atatgaagat ttaggagctg aagctataag aaagattgta gtagaagacc ttaagcttac
    10501 agtagcaata gatagctatg gaaataatat atatgagcaa ggtcgagcaa tttatgaatg
    10561 taaataatgt ttagaaataa atatgaatat caaaattaaa aatagttgtg tatattttaa
    10621 ggaggaaaat tgatgtctaa ggattatgca aaattggcat tagaaatgca tgaaatcaac
    10681 aaagggaaag taagtgtaga aagcaaggta gaagttaaaa ctaaggatga tttatcaaca
    10741 gcatatacac caggggttgc tgaaccttgt ttaaaaatac atgaaaatca agatgatgtt
    10801 tatagataca cttctaaagg gaatctagta gcagtagtta gtgatggaag tgctgtactt
    10861 ggattaggta acattggtgc tgaagcatct atacctgtta tggaaggtaa agctatatta
    10921 tttaaacaat ttgcagatgt agacgctttt ccaatatgtt taaagactaa tgatgtagat
    10981 gagatagtta aatctgtaga gttgatggag cctgtatttg gaggtataaa tttggaagac
    11041 atatcttctc caagatgttt tgagatagaa gagagattaa aaaaatcttt aagtatacct
    11101 gtttttcatg atgaccaaca tgggacagca ataatagttg cggcagccat aataaactca
    11161 ataaagttaa ttgaaaataa aaaaatagaa gatttagaaa tagttataaa cggagcagga
    11221 gctgctggta tagccatagc aaagatacta cttaatatga atgttaaaaa tataatctta
    11281 tgtgatagaa gtggagcatt ggaagctagt atagaaaatc taaactatgt tcaaaaagaa
    11341 atgttaaaag taacaaatat aagaaatgaa aaaggacctt taaaagatat tataaaagga
    11401 aaagatgtat ttattggtgt ttcaggtcca ggagctgtta caaaggaaat ggttgaaagt
    11461 atgtctgaga agccaataat attggcaatg gcaaatccta ctccagagat aatgccagaa
    11521 gaagcaaaat taggtggagc atttataatg ggaacaggtc gttcagactt ccctaatcaa
    11581 gtcaataatg tactggcttt tcctggaata tttagaggtg cacttgatgt taaggctaaa
    11641 gagataaatg aagaaatgaa gatagctgct gcatatgcaa ttgctaatac aataagtgat
    11701 gaagaaatat gcccagagta tatattacca gatgtattta ataagaatgt agtaaaaaat
    11761 gttgctaagg cagttaaaga agcagcgata aaaaccaaag taaatagaat ataaattttt
    11821 atatgttcat atatccttat attatcgggt atatgggcat ttttattata ggtttatact
    11881 catatatcct tacttttata tacatatctt tatgattaaa ggtgttagaa aatgtagtaa
    11941 attttatact atcagactct atctttatta ctttagtaga tacatggttg taaaggttta
    12001 tagaaaaaaa tataggaagg cagattttta ttgttgcgta tatatgagaa tgatataata
    12061 tgaatattat aaattaataa atttaaattt atggaggtac aagagatgat ggaatataaa
    12121 gttaatgacc aagaacttaa tgcttcaatg tttatcccat tcgtcaacag gatatggcca
    12181 ggagattatg atatggataa aacacagact gcattatcaa aaactttgaa tataactgct
    12241 tatgatgata aagtgcttgt aggatgtctg cgagttcttt cagatggtta ttattttgga
    12301 acaattacag aattgcttgt tcttccagag tatcagaaac aaggcattgg tagtaagctt
    12361 ttccagcttg ctaaagagaa caccccaact atgctatatt ttggtgcaca gccaggagta
    12421 gaagagttct atgaaaaaaa tgggtgccaa agaagtctgc aatcctacat aataaaaaag
    12481 aaagatagtt attaaagttc cagctttcaa taatcttaaa agtacaatat tttagtggaa
    12541 gcatttcatt ttttattact aatatgacat aaacatgtca tattagcatt gatatacttt
    12601 taatatggtt aagagccaaa gaaaaatata aggagtgatt taaaatggaa gaaactaaat
    12661 tttgccaatg ctgtgcaatg ccaatgggac aaactgatga attatatggg acaaataaag
    12721 atggaagtaa aagtactgat tattgtagtt attgttttaa agatggtgag tttacagctg
    12781 atataagtat ggaagagatg atagaagttt gtatccctca tatgcttcaa agtaataagg
    12841 atatgactga agatgaagca agaacaatga tgaacaattt ctttccaaca ttaaaacgct
    12901 ggaaataatt ttatgcaaaa ttagttatat gcaaaacatg aaatatggtg ttgagaataa
    12961 ggggtggtgt gctttatagc ataccacctg ttttgttact tggagttcag ttattttcct
    13021 aaatatttta aatatactat tttagataag ttccctcttc atcttgataa attaatcctt
    13081 ttttcataag cactccaaga gctcgtttaa aatttttctt acttgaatta aataatatag
    13141 aaatatcttt aggatcagat ttatcattaa atctcatata tccatcagaa ccttctaaat
    13201 aacttaaaat cttattttct aaagtatcaa gttcatcctt tctatttcct ctaggagaaa
    13261 gaccaagttt tccatcttca tatattttta tgacatgtaa attttcaaga acatcaccag
    13321 tttttagttc agtaaagtac tcattatgaa gtattacacc agcatattta ttgtctacac
    13381 atatcattgc agaattatta gtttggaatc catatacaac tccagttaca atatctccta
    13441 cattgtaagt atggtctgtt aataaatatg aatctatatc tgtagtagca gcaattcttt
    13501 tgcttttgtc taaatatata taaaataaat atttttcgtc tctaaatagt ggataagtct
    13561 tagctttaaa aggaactaat atgtctttag gaagtcccat atctataaaa gtaccaatgt
    13621 ctgtattatc aactactttt aaatgtgcta catccccaac cttagctaaa ggcggtatta
    13681 aagttgcagc agttctgtca tcagaatctt taaatatgaa agcatttact tcatcaccta
    13741 tgtttatttc attttttccg attaatctat tatgaagaag tatatcgtct tttgtgttat
    13801 tagtttgtcc atccaaaaaa tatccaaatt cagtttttct ttttactgta agtttattaa
    13861 aatcacctat gtttatcaaa ttaaaatcat cctctcattt tatatttata atttagccat
    13921 aatatactta tgtagtagtt tgtcacagta tctatattag tattagtgaa atttacaata
    13981 aataatatag ttatttatgt atttttcaat tctctagctt cttatatagt ataaactcca
    14041 atttcttttt tgtctttagg ttttattaaa atttttacaa aataagttaa aaagtattga
    14101 aataaaattt cttttaaagt ataattggta tataccaaaa ttggtatata ccatatcgaa
    14161 aagtggtgac tatatgaaaa tagtaaataa atctagtaat ataccattgc atactcaatt
    14221 aagttcaata ataagagaga tgatagaaac tggtgagctt aaagagggtg atgcaataat
    14281 gccagaaaga gaactttgca acatacaaaa tgtaagtcgt atgacagtaa ataaaaccat
    14341 tgttggatta gttacagaag gacttcttta tagggtacaa ggtaaaggga cattcgttgc
    14401 aaagcaaaaa aagaagtatc agttttcaaa tgtaaaaggt tttactgatg ttatgaaaga
    14461 aaagggagta aacataaaaa cggatatatt atcctttgag atggaactac cagatgattt
    14521 agttaaaaga aagcttggaa taagtgataa tactacaaat atatataaga ttgtcagact
    14581 tagatatact gatggagagc catttggatt agaaatagta tatctatcag aagaaatgtg
    14641 taagggtcta acaaaaggga tattggataa ttcttcatta tatagggttt taaatgaaaa
    14701 atatggctat aagatacaaa aagctgaaca ggtaatggag ccagtaatac tttcagatga
    14761 agaaagtaag cttcttgaaa cagatgaagg tgctttagct cttaaacttc atagaaattc
    14821 ctataataga gaaggtagcc ctatagaata taccatatca atatttagaa cagataaata
    14881 tcaatatgaa atagtattga gtgagtaggt gaataatatg aaatgtttaa ttaatggaaa
    14941 gataatatta aaaaatcaaa tcttagaaaa taaagtatta gtatttgatg aaaaaataat
    15001 tgatatagct gatagtgtac caaaagattg tgaagttata gatgctgatg gaaaatatat
    15061 atctccaggg cttattgata ttcatataca tggaaacatg ggaaaagata caatggattc
    15121 gactgatgaa tctattgaaa caatttcaaa gtcaattatg agacatggag taacatcttt
    15181 tcttccaact acaatgacta tggataaaga acatgtttat gatgcacttg aagttattaa
    15241 aaaagcccaa aatagaaagc ttgaaggggc acaagtatta ggagcacatt tagaaggacc
    15301 ttttataaat gagaattata agggagcaca aaatgaaaaa tttataataa attcaaaata
    15361 cgaatttata aaagaatata aagatgttat aaaagttata acttatgctc cagaaaaaga
    15421 tattgatttt gattttacta gagaaataaa aagatgtaca gatatagttt tatctattgg
    15481 tcatagtaat gcaaattatg accaagctaa agaagctata aatttaggag taactaatgt
    15541 aactcacatg ttcaatgcaa tgactggttt aaaccataga gacccaggcg ttgttggtgc
    15601 agcacttaca actaatgtgt acagtgagtt aatagcagat acaatccata taaataaaga
    15661 tttatttcaa tttatactaa ataataaagg aaaagaaaga ctgattttaa taacagattc
    15721 tatagaagct ggtggtcttg aagatggcaa ttattctctt ggaggtcaga aagtcatagt
    15781 taagggtaat gaagctagac ttgaaaatgg agcattagct ggaagtgtgt tgtctttaaa
    15841 taaaatggta tttaacttct tagataatac aaaccttaaa gtgaatgaag caattaattt
    15901 agcttcttta aatccagcaa catctttggg aataaatgac aaaaaaggaa gcttagaaat
    15961 aggtaaggat gcagatatag ctgtatttga tgagaattta gattgtaaaa tgacactttg
    16021 cttgggggac gtagtatata aaaatatata gtatacaaaa caaaccttaa agacagtaaa
    16081 aacaatataa aaaataataa attaaactta cattagtaaa agtagtagtt aaaatttagg
    16141 aggaataaga tgagagtatt agtgtgtaaa gattatgatg gaatgagtaa aaaagccgct
    16201 gaaatgatag cagctcaaat agttttaaaa ccaaattcaa tactgggact agctacagga
    16261 agtacaccag taggtatgta tagagattta gtaaaaaaat acaatgataa tatagtagat
    16321 ttttcagatg tgatgagctt taatttagat gaatattata agttaccaat atctaatgac
    16381 caaagctatg attattttat gaaagagaat ctatttaatc atgtaaacat aaagccagaa
    16441 aatactcatc taccaaatgg aatggctgat gatattgaaa aagagtgtat gaactatgaa
    16501 gcttctatag atgcagctgg tggtatagat gtacaagtac ttggaatagg tcgtaatgct
    16561 catataggtt ttaatgaacc tgatactaaa tttgcaaaga gaactcatgt agttgaactt
    16621 acagagtcta caatagaagc taatgcaaga ttctttaaat caagagaaga tgttcctaaa
    16681 aaagctgtaa gtatgggaat aggttcaata ttgaagtcta agaaaatatt gcttttagct
    16741 tcaggagaag aaaaagctga tgcagtatat aatactgttt atggagatat aactccagaa
    16801 gttcctggtt ctatacttca acttcataaa gacactatag ttatagtaga tgaagcagca
    16861 gcaagtaaat tgaatccaaa agattataaa ttagtataag aatagaatgt agtttaagta
    16921 aaaaatagct atcattgagt aatcaaattt atttaatgat agctattttt atttaaattt
    16981 tttacttctt tttatttaaa ttagttatta ttgtttaaat tacttgctta ttacttttta
    17041 cgaaaactcg ttatcaagaa gtatccaaat aagatgttaa aaattattat gtttagtggg
    17101 attctatcaa gcataataaa attacctata attgggatat taccaactgg aaacataaag
    17161 aagaatacag gcatcagtga tagtaaaaaa gatggaaaac aaagaagaag taatcttgga
    17221 atatcaatat accaactacc atcctttttg tattctctaa tcaataattc agttcctagt
    17281 aataaaccaa taccaccaga ggaaattaaa ttaataaaaa tgtatctttt aaagtcttgc
    17341 atataaccag tgtaaaattg agtatagaaa tcataattta atttaacggc taaaaaatac
    17401 ataagcaata gaatgattaa aaataaaaga tatctaagag cttttttcat tttaaattct
    17461 cccttcagaa atactttatt tagttttatt ttgttggaat ctgattttat catttagttt
    17521 aagaaatcca taatattagt gaagaatgaa ataagtatta tatatgaata aatattttta
    17581 gaattaaggt gaattgtagt gcttaatata actataatat taattgtttg aatattcaat
    17641 aaaaagtttg aaaaatatca attctgatat aaactgaata aatacagttg aatattaaat
    17701 tattatttta tcaaaaatat gcaaagtact acaccttcaa atatcaccta aagtaattaa
    17761 aaggcaatta aggtgaagta gttcaagtat ttctaaaata agtttaataa attctataat
    17821 aatttatttt ttgccttaaa attgccttaa aatccaaaca attttaaaac taatgtacta
    17881 taaaaagctg gtataattta aatatagtaa aaaataagat ttatcttatc tattgggatt
    17941 taattattta atcaatatat ttataccatt taatttaaat aaaaagtcta ttaaggagtg
    18001 agcttatgag taagttaatt tatatagcag atgatgaaga taatataaga aatttggtaa
    18061 agacattctt aaaaaatgaa ggtcatgatg ttatggattt taaaacgggt gatgaacttt
    18121 tagaacaatt taatataaag gagtgtgact tagtaatact agatataatg atgcctggct
    18181 caagtggttt tgaagtatgt acaaaacttc gtgagaaaag tacagtccca ataatcatgc
    18241 ttactgcaag agatacagat atagattata ttacagggat aaccttaggt agtgatgatt
    18301 attttacaaa accatttagc ccaatgtctt tggttatgag agtaaaatct atatttagaa
    18361 ggatagagtt tgaaaagaaa caaaattatg ataaatatag taactcgata gatatggaat
    18421 taaaatttgg tgatgtaata ataaataaaa agaataaaat cgttacaagt aaaaatgtaa
    18481 atatagacct tacaccaaat gaatataacc tattgactta tttgtttgaa aatatagata
    18541 gagcagtttc cagagatgaa ttattaaaca aaatatgggg atacgatata gaagttgaga
    18601 caagggctgc tgatgataca gtgaagagat tacgtaaaaa aattttaaat acaaatattt
    18661 taatagaaac tgtatggggg tttggattta ggttgaagga gaaaagttaa gatgaagaat
    18721 ggaaatgaca agaatagaat tggaaagatt aaatccaaat ttaaattaaa gttctcaaag
    18781 aaaaatataa gcataaaaac taggattttg ttgagtatac tttgtttgat aattatagta
    18841 tttatgataa tattagtatc ttttaatgta tttcttgata catatataaa aacaactgcg
    18901 aatgaagaat tgacaaagtc tacagaaata gtagaacata tggatagtaa ttttagacct
    18961 attaagccac cacaagatga tggaaagctt cctcccaaag tattatctaa ttttatgaga
    19021 aatgtacaag ataaagttag gatggcagaa acacaatcag atgctgatgc tatggtagtt
    19081 gattctaagt ataacctgat atttccaaca agagaggatg atttcttaaa aaatgttgat
    19141 gagatggagc tgattaggga gtgtattcaa aatgaaaaat tagatttaaa ttcagatgaa
    19201 aatacacgga tatctaccag aaataaggat tattatattt caactgtaaa aattactagt
    19261 atatatggtg aacaagacaa gtatcttata ctttttatag acatatcaaa aactctaaat
    19321 ctagcacaaa agataaatat tgtattaata tctgtaatgt gttttgctgg aatattagca
    19381 atatttacag ctgtgatttt atctgagaaa attgctaaac cgataaaaga gttgtgtgag
    19441 tttgcaaaaa aaatgggtca aggagatttc aaaagaacct actttgactt ttcagataaa
    19501 gaattagtag agctctcaac tgttatgaat aagagtgctg agtatctgga taagtatgat
    19561 aatgaacaaa aaatattttt tcaaaatgca tctcatgaat taaggacacc acttatgtct
    19621 attaaaggtt atgcagaagc tataaaatat aatgttatag atagtaaaca tgcaagtgat
    19681 ataattcttg aagaaagtga taggttaagt gatatggttg aagatttatt atacatttct
    19741 aaaatagata atataactaa agattttgaa cttgtagaat gtgatttaag agaagtactt
    19801 tcaaattgtg gagctaggca aaatgtaaga gctataaata aggggataaa atttatttat
    19861 gaatttgatg aagaaatggt tttatttgaa tgtgatgaaa aaaacatttc caaagcattt
    19921 atgaatctta tagaaaatgc tttgagatac gctaagagtg aaataaaaat agtgtgtaag
    19981 tataatcaaa aaaatatagt tgtgataata gaagatgatg gaattggaat caagaaagaa
    20041 gatttacctc atgtttttga aagattctat aagggagtag gaggaaatca tggaattgga
    20101 ttatcaatag ttaaatctat tgtaaataag catggtgggc gtatatacgt agaaaatggc
    20161 aaaaaaggag ctaaatttac tatattattt aaattgtaaa aaaattagag aaaatataga
    20221 gatgtttgaa gttgtttttt atatatatct gttgtatact gttgaaaaaa gaacaatatt
    20281 aagctgacat atattacgat agttgacatt taaagtaaat gcttaaatat agatacgaaa
    20341 ggaaaagtaa aatctgaagt gtatttaggt ttaagtaagc ttcttatttt ataaaaaata
    20401 tgtatgaaag aacagctgat ttttaatatt acaaatttag taacggttgt atttgaagct
    20461 tttgtcatcc atatgttttt atcagacttt ttaggtcaga aagaagggta tataaaagtt
    20521 gtaagatatg ctaaactggg ttttataata tgtttaggat tctgtaattt aataacttta
    20581 aaccctaaaa tcacaatgcc tttaattttc atccttattt tttcaacatc atttttatat
    20641 aaaggaaatt taaagacacg attgtttact actgttttat taagtatatt ctttatactg
    20701 tcagaaattg tagttacaag tatttttgtt ttatttgtaa aagaaggctt tgaaataatg
    20761 cttgaaaata atagtataag agtattggcg acaattttat ctaaaattgt ctttttgttg
    20821 acttgtaaaa ttatttgtct atttaaaaag gatgtacatt tagatatgcc cataaagtat
    20881 tggctccctt tatttttaat acctatattt agtttatttc tgtctgtttc tatctttgat
    20941 gtaagtaaat tttttagtct tgaaagttta aaatttctaa gcttaatatc tagtgttggt
    21001 attttatata ttaattttat tgtcttttat ttatttaaat ttattataga taaaactaaa
    21061 ctatctatga aatatgaact acttgaaaaa gaaataattc ataaggaaga attaagactt
    21121 agtaatgagt gttataaaat tattgttgaa cagactgatt ctgtcgtttt cgaatggaat
    21181 atcaaagaaa ataaatcatt tgtatcgcag gcttggactg aaaaatttgg atataacaat
    21241 gcatgtaaga atatttttaa agaaattaaa gataaagact tagttcattc tgaagataaa
    21301 gctatatttg aaggttttct tgaaagtatt aaaaaaaaga atatgcacaa tcaagcagta
    21361 tatcgtttaa aaaaatctaa tggtgaatat atttggtgta gaacatcaat tacaagcatt
    21421 tataatgatg aaaatgaact tttaagagtt gtcggagtaa ttgtagatgt agatagtgat
    21481 ataaaaaaat atgaagagtt aagaactaga gcagaaagtg actcattgac caatatatac
    21541 aataaaggta catttgaaaa attagtggaa gaaactatag ttatgaatac aggtgataaa
    21601 aaagatgcat tattcattat tgatttggat gattttaaag aaataaatga taattttggt
    21661 catccatttg gtgattttgt actaaaaaca tttgctgata agatacaaac tagttttggt
    21721 agtaaagact tagtcggaag aattggtgga gatgaatttg ttgtatatat gcaagattat
    21781 gttacagaag taaatttgca taaaaaagct aaagaattaa atagagtact ttcggataat
    21841 tacactgatt tgagttttag ttttgatgct tctgtcagta taggtattgc aaggtatcct
    21901 caagatggaa cttcattttt tgaattgttt aaaaatgcag atagagcact ttatagcata
    21961 aaagcatcag gtaaaaattc atattgttta tttgaagaag aactatatgt acagtgaatt
    22021 tgaatttaga ttttatgtga gagtaagatt tatatatact tgtggctgtc tgatttataa
    22081 aatatgttac tttgtatatt tttatatgtt tcagtaaggt taattaacat gagcaaaggt
    22141 gtaagtaaat ttatattagt ttaagtttac ttacaccttt ataaaaatac aaataataga
    22201 ttcatttaca ttcttaaatg attaaagctc tttaccttct aaacatgcat aacatatcgt
    22261 tataattcca gtaaataaga ctaaaatgcc cattactaag attacaattt catcatctat
    22321 tactccaaat gtttttaaag taacttattt cgctttgtat cataataatt tgtctctttt
    22381 tacattttaa cttctgctga gatttgagtt agatattcat tagaatctcg cacacttata
    22441 tcatatagaa gatattcttc ataaacaaaa tccccaatac gtaaattatt ctgattaaaa
    22501 aaatttaata acttttcata agccttgtaa atagtttcat aactcccttt atgataagca
    22561 gttacataaa gtccctttgg tttgattgat acaggatatt tcttgtactc tttattaact
    22621 tttgtaaata aatatgaata tgattcaaag tctttattaa ttatatcttc acctttaatg
    22681 attgagccaa tcgtaccata atcactcaat tcatagttgt ccataaagtt aatacattca
    22741 tataaaaaat tttcttcctc atctcctaaa aactgttctt tatatgtaac aggtgttttg
    22801 accaagtact cttcttcatg ttcttttaaa gtaatagtat tcgcatctat attacatgca
    22861 cttttagtaa atgaaatagt agattccatt agagcttgaa tattatttag tttttcaatt
    22921 tcatttttaa tatcaatggt tttcttttct aaaagctcaa ttagtaaatt aggtgttctt
    22981 ttgtctatat aagctttaat ttcttttaaa ggcattttta cttccctaag tgaagaaatt
    23041 ataccaaaaa tgctaagttg atggtatgag tagtatctat atccattttc tttctttaat
    23101 tctggtgaga aaagacctat atcatcataa tggaaaagtg tttttttatt gattccacaa
    23161 atctttgcaa attctccagt tgtaaaataa ttttttgaaa cttgactcat aattataatt
    23221 cctccttgac tatcctgtta cagtatagtt tatcatagta ttttgtaaag gagtgtgatt
    23281 atatgaaaaa attaattcat tttttaaaac catatagagt tttaattgtt gtagttttga
    23341 tatttacgtt tttacaaaca cttggaactt tgtatatacc aacacttaca gcaaatatag
    23401 taaataatgg tgtagtaaag ggagatatag attatattgt aaaaacaggt ttaatgatga
    23461 tgatagtagc tggtataaca gctttatcag ctgtgttagt ttgtaaagta tcagcaaatc
    23521 tttcatctgg attttgtaga gatattagag aagcagtatt tataaaatca caagatttat
    23581 caataaatga ttttaataat ataggtacag catcaatgat tactaggagt actagtgata
    23641 ttactttaat aggtcagtct gtgtttatgt ttattcaatt agtattacca gcaccaatta
    23701 taactgtttc tggtctattc ttagcatatt ctatagataa agcaatgaca attattattg
    23761 ttgtagtaat gtttttattc atgctttcag catttttagt tggtaaaaag cttataaagt
    23821 tgtttaaaat gatgcaaata aagatggata atatgaatag ggtgttacgt gaggtagtaa
    23881 ctggagtaag agttataaga gcttttaata ggagtcgttt tgaaaaaaag agatttgata
    23941 gaactgcaat agattattct gaaacagcaa tttcaataaa taaaatattt gcagtactta
    24001 tgcctattgt aatgttaata atgaatttag gtatagtaag tattatttgg tttggtggga
    24061 tgagagtcag taatggaaat atggaaatag gtcatataat ggcacttgtt gaatattgta
    24121 tcttaatttt attctattta atcatgggag taatggtatt tatgtacata cctagagcag
    24181 gtgcatgtgc agatagagta aatcagatat tagatataga acctgaaatt gtagatggaa
    24241 atggtcataa agatactgtc tcagagagag gacatttagt atttaaaaat gtaacctttt
    24301 catatgcaca atcagaagaa cctgtgttaa ataatataac atttgaagca aaatctggag
    24361 aagtaactgc aattatagga agcactggct caggcaaatc tactattgca aatataatac
    24421 cacgtttttt tgaaattcaa tctggagaaa tatctataaa tggtcaggat attaagaaga
    24481 ttcctcaaaa agaacttaga gataagattg gatttgtacc acaaaaagct tttttattta
    24541 gtggaacaat agaagaaaat ataaggtatg gaaaagaaga tgcaagtatt gaagaagtaa
    24601 agcatgctgc aagtatagct caagctgatg aatttattta tgatatggaa gacaagtatg
    24661 actcatttgt agcacaaggt ggaaataatt tgtcaggtgg tcaaaaacag agaatatcca
    24721 ttgcaagagc gttagttaga aagcctgaag tatatgtctt tgatgatagt ttttctgcat
    24781 tagattttaa aacagataaa agacttagaa aagcgttgaa aaatgaaatc aaagattcat
    24841 cagctataat tatagcacaa cgtataagta caatcatgga tgctaatcag attatagttt
    24901 taaatgatgg aaaaattgta ggtattggaa aacataaaga tttacttgaa aattgtgaag
    24961 tttataagca aattgcggat tcacaactta gtaaagaaga acttgcataa aatgaagaat
    25021 taatctgata tttggaggtg tatgatatga gtgaaaaaag aatagatgaa caagatatag
    25081 aagtacttag ttttgaagat ggcatgtcag cagaaaaagc taaggatgca aaaaaaacta
    25141 ctaagagatt attaaaatat atggcaaaac aaaagtttaa aatattaatt atttttattt
    25201 cagtattgat aagttcagcg cttacagttt tagcacctat ggttatggga aaagctattg
    25261 accaactatt taatggaata aaaactgcag tccaaacagg tacaaaattc agtgtgaatt
    25321 ttagtactat gggagggata gtatcaatac ttctagggtt gtacttaatt agtgcagtat
    25381 ttatatatat tcaacagtat attatgtcag gagttgcaca aaatctagtt cttagcatga
    25441 gaaaagattt aagtgataaa ctcaataaac ttcctcttaa gtactatgat tctcataaaa
    25501 agggggaaac acttagtata gtaacaaatg atttagaaaa agtagcagat agtttacaag
    25561 aaggattaat gcaacttatt acagcagtag ttacagtagt tggttctatt gtaatgatga
    25621 taagtataag tgtaccactt acaatagtat cagctataac attacttgtt tcattgggta
    25681 ttactgttgt aattgcaaga aaatcacaag aaagattctc tgaaaatcaa aaggcactag
    25741 gagagttaaa tagcaatata gaagagatat ttactggtca aatagtggtt aaagcattta
    25801 gtaaagaaaa ggatactata agaaacttta aaatattaaa tcaaaatctt tataatgcaa
    25861 gtagaaaagc acagttttcg tcatatgcaa tatctccaat tattagattt ataaacctaa
    25921 ttggctatgt tattatagca gttgtaggtg ggatatttgc atcaactgga gcaatgactc
    25981 ttggtagtat acaagcgttt attcaatatg taaatcaagc atcagagcct actacagaaa
    26041 tatcttatat tgtaaatatg cttcaagcag caatagcatc tgcagagaga gtttttacag
    26101 ttatggacga ggtggaagag ataaaggatg aagaatcttc aaaggttatt agcatgccta
    26161 aaggtaaagt acaatttgag catgttaaat ttggatatag tgatgatttt attcttatga
    26221 aagatattaa tatcaatcta aatgcaggag ataagattgc aattgttgga cctacaggag
    26281 ctggaaagac aactcttgta aatttactta tgagatttta tgagatacaa ggtggaagaa
    26341 ttactattga tggtgttaat ataaaagatt taaagcgtgg agaacttaga actatgtttg
    26401 gtatggttct tcaagataca tggctattta atggaagtat aaaagaaaat attgcatata
    26461 gtaagtcaga tgccacaatg gatgaaattg taagagcagc aaaatcagca agagtagacc
    26521 attttataag gactttacca caagggtatg acactatatt gactgaggat gcttctaatc
    26581 tatctcaagg tcaaaagcaa cttctaacaa ttgccagggc aatactttca gacccatcag
    26641 tattgattct tgatgaagca acatctagtg tagatactag aacagaggta gaaattcaaa
    26701 aggcaatgaa taacttgatg aagggaagaa ctagttttgt tattgcacat agattatcaa
    26761 ctattcgaga tgctgattta atattagtaa tgaaagaagg aactattatt gaacaaggaa
    26821 gccataagga acttattgaa aagaaaggat tctatgaaga actttataac agtcaattta
    26881 caagtgaata tgatgaagat gttgtatgat tatcaagttt taggcaacta aatgattgat
    26941 aaatattaag ttaaatatag taacatgtaa ggttatatgg aataagagta tttctctatt
    27001 tcatataacc ttttttattt atcaaaataa gataaaattt cctttctaaa ttagattgaa
    27061 gaatgtattt atcaatataa aagtaaaata atcattatta ttagtgaagt aaatattgtt
    27121 tgccacatat atgccataaa gatgcactta ttttacggag atttaacaaa gtagtgtaaa
    27181 gtatactcta aatataaaat aaatcaaata gagttaaata aattgagagg tgatataaat
    27241 agtgaataaa aaattgaact atagatttga aatgaagcac aaaataactg aagcagatgt
    27301 gttagcttta aaatcaagac tctatccaat aatgaagaaa gatgaaaatg catctaaaga
    27361 tggaaaatat cttattagga gtttatattt tgatactcct gaagataaag cacttttgga
    27421 taaattaaat ggcgttgcaa taagagaaaa gtttaggata agattttata ataatgactg
    27481 ctcttatata aggttagaga aaaaaatcaa acattacaac atgacatcaa agctatcaac
    27541 taatttaacc aaaaaagaag taataaatat actaaataat gatattgaat ttctaaaaga
    27601 gtcgacaaat tgtttattaa gagaatttta tttgaaatta aaatgtgaaa gattagaggc
    27661 aaaaagcata gttgattaca atagagaagc ttttatttat ccagttggaa atgtaagagt
    27721 tacaatcgat agtgatatta aaacttctgt aaattcggta gatttattta ataaagattt
    27781 gccaactgtt tcggtaattg atgagaatat gactgtattg gaggttaaat atgatgaatt
    27841 tataccagat tttataaagg atttaattca aattaataag actacctcta cagcagtttc
    27901 aaagtatgct tcaagtagat tatatattta agaataaagg ctatgattta tataaagata
    27961 tacataaata agattaaaaa acaaataagt taaaaataaa aaataaataa gttaaaaata
    28021 ggaaggatga ttttaattat gacatttaat gatattttta aatcaagttt tatagaaaac
    28081 gtatcaggtt tttcatttgt ggacagtgct ttagcactag gttcagcttt tttagtaggt
    28141 ttgtttatat atatggtcta taaaaagact tatatgggaa ttatgtattc tcgaccattc
    28201 aatgtgtcgc ttgtagcact tacaatgctt acaacttttg taatattggc agttacttca
    28261 aatgtagttt tatccttagg tatggttggt gctttatcta tagtaagatt tagaacagca
    28321 attaaagacc caatggattt agtatttcta ttttggtcat tgggttcagg tatagtttta
    28381 ggagcaggtt tgattccctt agctgtcatg ggttcaataa ttatgggatt gattctaata
    28441 ttcttttcaa ataaaacaat atctgaaact ccatatatat tgatggtaaa ttgtaataat
    28501 gaagattcag aagatatagc aactgataaa attaaaaaag tatttactaa atatcaaata
    28561 aaatcaaaaa gtgttacacc agaaaaagga atagaattag tgtttgaagt aagaatgaaa
    28621 gatggagaaa caagtcttat aaatgacttg agtcaagtag atggagttac taatgcagtt
    28681 cttgtaagtt ataatggtga ctatgtagca tagagaggag taatctttta tgaaagataa
    28741 aaaatttacc cttcttatct cgattatgat tatattttta tgtgctgtag ttggagttta
    28801 tagtacatct agcaacaaaa gtgttgattt atatagtgat gtatatattg aaaaatattt
    28861 taacagagac aaggttatgg aagttaatat agagatagat gaaagtgact tgaaggatat
    28921 gaatgaaaat gctataaaag aagaatttaa ggttgcaaaa gtaactgtag atggagatac
    28981 atatggaaac gtaggtataa gaactaaagg aaattcaagc cttatatctg tagcaaatag
    29041 tgatagtgat agatacagct ataagattaa ttttgataag tataatacta gtcaaagtat
    29101 ggaagggctt actcaattaa atcttaataa ctgttactct gacccatctt atatgagaga
    29161 gtttttaaca tatagtattt gcgaggaaat gggattagcg actccagaat ttgcatatgc
    29221 taaagtctct ataaatggcg aatatcatgg tttgtatttg gcagtagaag gattaaaaga
    29281 gtcttatctt gaaaataatt ttggtaatgt aactggagac ttatataagt cagatgaagg
    29341 aagctcgttg caatataaag gagatgaccc agaaagttac tcaaacttaa tcgttgaaag
    29401 tgataaaaag acagctgatt ggtctaaaat tacaaaacta ttaaaatctt tggatacagg
    29461 tgaagatatt gaaaaatatc ttgatgtaga ttctgtcctt aaaaatatag caataaatac
    29521 agctttatta aaccttgata gctatcaagg gagttttgcc cataactatt atttatatga
    29581 gcaagatgga gtattttcta tgttaccatg ggattttaat atgtcatttg gtggatttag
    29641 tggttttggt ggaggtagtc aatctatagc aattgatgaa cctacgacag gtaatttaga
    29701 agacagacct ctcatatcct cgttattaaa aaatgagaca tacaaaacaa aataccataa
    29761 atatctggaa gagatagtaa caaaatacct agattcagac tatttagaga atatgacaac
    29821 aaaattgcat gacatgatag catcatatgt aaaagaagac ccaacagcat tttatactta
    29881 tgaagaattt gaaaaaaata taacatcttc aattgaagat tctagtgata ataagggatt
    29941 tggtaataaa gggtttgaca acaataactc taataacagt gattctaata ataattctaa
    30001 tagtgaaaat aagcactctg gaaatcaaag tgatgaaaaa gaagttaatg ctgaattaac
    30061 atcaagcgta gtcaaagcta atacagataa tgaaaataaa aataaaacta caaatgatag
    30121 tgaaagtaag aataatacag ataaagataa aagtggaaat gataataatc aaaagctaga
    30181 aggtcctatg ggtaaaggag gtaagtcaat accaggggtt ttggaagttg cagaagatat
    30241 gagtaaaact ataaaatctc aattaagtgg agaaacttct tcgacaaagc aaaactctgg
    30301 tgatgaaagt tcaagtggaa ttaaaggtag tgaaaagttt gatgaggata tgagtggtat
    30361 gccagaacca ccggagggaa tggatggtaa aatgccacca ggaatgggta atatggataa
    30421 gggagatatg aatggtaaaa atggcaatat gaatatggat agaaatcaag ataatccaag
    30481 agaagctgga ggttttggca atagaggagg aggctctgtg agtaaaacaa caacatactt
    30541 caaattaatt ttaggtggag cttcaatgat aataatgtcg attatgttag ttggtgtatc
    30601 aagggtaaag agaagaagat ttataaagtc aaaataagat taaaattgaa tataaataat
    30661 tttaatactg ttaagtctaa atatttactt agcagtattt tttagaaaat aatgtatttg
    30721 gttactaata taaggtaata aattgataaa aattaaatac agcatattaa taatacataa
    30781 taaagtaata aaaaaactgt ttaagtataa taacttatat ttagacagtt tttttcattt
    30841 ataaataata taaatgaaat tgaaaacatt tgaaggatag aaattggttg gataaatgta
    30901 taaattactt caccaaaaaa taatgacaaa aattgtaact aagttgtaat aaaatggtag
    30961 ttatttagca taaaataatg tatcatagaa gtatattata aaattaataa aataagattt
    31021 ttgtaagaaa actgctggct tttgatattt agagtgagta gttaaattat gtttaggagg
    31081 ggtagaattt gttgcaaaaa gtattatgga aatttttaaa gctagtagtc ggtttattta
    31141 tatgttcagt tggtatagta ttgacgataa attgtaacct tggattatct ccctgggatg
    31201 tatttcatca aggactttca aatcatatag gaataactat aggaactgct tctataattg
    31261 taggctctat agtagttatt gctgatgtcg ttcttggaga aaatgtagga tggggaactg
    31321 tttttaacat gctactaatt ggatttttta tggatttact tttatatagt aatttaattc
    31381 cagaggctga taatttattt gtaggaataa ttatgttgat attaggattg gttcttttgt
    31441 ctgttggaat ggtattttat atgggaagtg gtttaggaag tggacctaga gatggtttga
    31501 tggttgcaat tcaaaagaaa acaggaaaat cacttaaatt aataagaggt actatagaag
    31561 ttggagcatt aatagtagga tttttattag gtggaaaagt tggtatagga actataataa
    31621 gtgcatttgg attggggtat ttcacacaaa tggtatttgg cttatttaaa ttagattgta
    31681 gtaagattaa acatagattt atagtagatg atataaaatt tatcaaagtg tatatgagtg
    31741 gtgagaaaaa agctgctgtt caaaatatag ttaagtaatt taagaataaa ttcaacgaaa
    31801 tgttaagtat tttaaattaa cattttgttt agatagataa attgaatatt aaaagaatat
    31861 taaaatatga ggattgctag aacactataa aatatagatt tctagtaatt ttttatttgt
    31921 tcaatatttt tttagtacta gaagtcttat tctctattta atgaatgaat taatttataa
    31981 ttagagatac taaactttat agatattaat ataaacacct ttaaactgtg tattttccaa
    32041 agcctttaaa atgcttgtat ttattctaaa taaaaaaata aaaaaatgtc aaaaaaactc
    32101 ttgtattata tccaaatttt atatataatt acctatgttg tttagaaatt ttaaactcaa
    32161 tgtttagtaa acaaaatgtt tagtaaaata aaacagaaac taggaggtgc aacatggata
    32221 taggtgaaaa gataaaaaga ttaagaacag aaaaacaact gacccaggaa gagttagcta
    32281 atagatgtga attgtcaaag ggatttattt ctcaattaga aaataactta acatcaccat
    32341 ccatagctac ccttatagat atacttgaaa tactaggtac aaatttaagg gaatttttta
    32401 atgaaataga tgatgaaaga atcagtttta caaaagaaga tatgtttgaa actgaagatg
    32461 aagatttaaa atataaatta aagtggttaa ttccaaattc acaaaaaaat gaaatggagc
    32521 caataataat aacattgtat ccaggaggac agtataaaga agaaaagcca catgaaggtg
    32581 aagaatttgg atatgtatta gcaggttcaa tatatgtcca tataggcgaa aagaaaaata
    32641 aagtaaaaaa aggagagagt ttttacttta gaccgaaagc aaatcactat atatcaaacg
    32701 agggcaaaac tacagcaaaa gtgatttggg taagtacgcc accatcattt tagaatagag
    32761 gtgtttaata attggtagaa aatataatag aattaaggga tatatctaaa cattatgaag
    32821 aacttactgt tttagataat ttcaatttag atataaagaa aaatgagttt ctaacattgc
    32881 ttggaccaag tggatgtgga aaaactacaa cattaaaaat aatagcagga tttgagtatg
    32941 ctgatgatgg taaagtttta tttgaaggta aggagataaa taatttgcca ccatatgaaa
    33001 gacaagtaaa cacagtattt caaaaatatg ctttatttcc acacatggat gtttatgaaa
    33061 atgtagcttt tggattaaaa attaaaaaga ctccaaaaga tgtaatagat gctaaagtaa
    33121 aagaggtttt gaaacttgtt gcactagaag gttttgaaag acgacaagtt gaatcattga
    33181 gtggaggaca acaacaaaga attgcaatag caagagctct agtcaatgaa ccaaaggtat
    33241 tattactaga tgaaccactt ggagcacttg atttaaagtt aagacaagag atgcaaatag
    33301 aattaaagag aatccaaaaa aaattaggaa taacatttgt ttttgtaact catgaccaag
    33361 aagaggctct tactatgtca gatactattg ttgtaatgta taagggcaaa attcaacaaa
    33421 tgggaacacc acaagatata tacaatgaac ctaaaaattc atttgtagct aagtttatag
    33481 gtgaaagtaa catatttgat ggaattatga tagaagatta taaagttaat ttctgtaata
    33541 gagattttga gtgtgttgat aaaggattcg aaaaaaacga aaatatagaa gtagtaataa
    33601 gaccagaaga tataaaaatg gtaaagccag aagaaggaat gttaaagggg aaagttacat
    33661 ctacagtgtt taaaggagtg cattacgaga tagaattgaa tgaaaatgga agattgtgga
    33721 tacttcataa tactaaaaat gctgaggttg ggactgaact aggcatggat atatatccag
    33781 aagatatcca tataatgaga aaagagaata actaagatga aaagaaaatc ttttttagca
    33841 tatccatatg tagtttggag tgcgatattt gtaataattc ctttaatatt agtagtattt
    33901 tttagtttta caaaagaatc aggtggaggt tatgccttta cacttgagaa ttataaagaa
    33961 gtaattgacc ctatatatat gaaagttttt gggcgttcta ttcttttagc aggaggagct
    34021 acattaatat gccttatagt tggttatcca gtggcatata taatttcaaa ggctcgtgta
    34081 agtagaagag gttcattaat attattgttt attcttccta tgtggatgaa ctttctttta
    34141 agaacatatg catgggttgc tatattagga aaaaatggac ttttaaatac atttttaggt
    34201 tggtttggaa tacaaccatt agcaattttg tatactaatt ttgctatact tttaggtatg
    34261 gtgtataact ttttaccatt tatggttctt ccaatatata cagcactatc aaagatggat
    34321 aatgatttga taaacgctgc tcatgacttg ggtgcaaata atatgacagt ttttagaaaa
    34381 attatatttc ctttgagttt gccaggtgta atgtctggaa taactatggt atttatgcca
    34441 gcagtcacta cttttgcaat atcaagactg ttaggtggag gtaaaataat gctggttgga
    34501 gatttgattg aacaacagtt tacagttgtt ggagattgga actttggttc agctatatcg
    34561 atatttatga tgatagtaat attaatatct atgtctataa tgtcaaaatt tggagatgaa
    34621 tctgacaaag aaggcggtgg gttactattt taaaattaaa gaaaataact tctaatatat
    34681 atttagcatt agtattctta tttttgtatg cacccatatt tgctttggta ctattttctt
    34741 tcaatgattc aaaatcaatg gcaagatgga atggatttac atggaagtgg tatggtcaat
    34801 tacttcaaaa tgagagtata atgagtgctt tatattatac tattgtaata gctatattag
    34861 cgtctgtaat atctacaata gtaggaacta taagtgctat aggaatacat aagatgagag
    34921 gtaagagtaa gaaattaata ctaaatgtaa actatcttcc tattttaaat acagagatag
    34981 ttacagcagt agctcttatg agtctgtttg tatttgtaaa aatggaattt ggatttacaa
    35041 caatgctatt ggcacatata atgttttgtt tgccatacgt tatactttca gttttgccaa
    35101 aaatgaagca attacctgat aatatagaag atgcagctat ggacttggga gcaacaccta
    35161 tatacgctct tagaaaagta attttaccac aaataaaacc aggtatagtt tctggatttt
    35221 taattgcatt tactatgtca atagatgact ttataataag tttctttaat gctggaaatg
    35281 gtgtaagcaa tctttctata gaaatctatg gaatggctag aagaggtata aaacctgaaa
    35341 taaatgcatt atcaacaata atgtttgcag tggttctagg tttactttta ctagctaata
    35401 aaaaagaatc tatagtaagg gggattaaat aatgattaag tttaaaaaag caatatccct
    35461 tatagctgta ggtatgatga cagttacttt atttacagga tgtaataaag caactgattc
    35521 tacaaaagtt ttaaatgtat ataatgttgg agattatata gatgagtctc ttatagataa
    35581 gtttgaaaaa gaaacaggaa tagatgttca atattcaact tatgatacta atgaaatgat
    35641 gtatcaaaaa gtaaagagtg gtagtacaca ctatgattta gtatttcctt cagactatat
    35701 ggtagagaaa atgaaaaatg aaggtctttt ggaaaaatta gattttaaga atatacctaa
    35761 tatgaaatat ttagataaga gttttttaaa tcctgtatac gacaaaacta atgaatatag
    35821 tgttgcatat atgtggggaa ctcttggaat attgtacaat aagaaagaag ttaaagaccc
    35881 tatggatagc tggaatatac tttggaatcc aaagtataag ggaaatataa tgatgtttga
    35941 ctctgttaga gatactatag ggataactct taaaaaatta ggatatagca tgaatagtgt
    36001 aaatccaaaa gaaataaatg aagctaaaaa tcttttaatg aaacaaaagg atttagtatt
    36061 ggcttatgta aatgatgaag gtaaggatag attgcttggt ggagaagtgg caatgggtat
    36121 gctgtattct ggagatgcag ttacactaat ggagcaaaat cctgaccttg attatgctat
    36181 acctaaagag ggaactaata aatgggtaga tgctatgtgt gtacctacaa cagcacaaaa
    36241 taagaaagaa gctgagctgt ttataaactt tttattagac ccagagaatg caaaagtaaa
    36301 tgctgagtac attgggtatt caacacctaa tacaggagca ctaaaattat tagacccaga
    36361 aataactcag aatcctgttg catatccatc taaagaagta cttgataaat gtgaaacttt
    36421 tgaagatatt ggtgaaaata taaaattata tgacaaggct tggatagagt taaaatcaaa
    36481 atagggtctt gttgttttaa gtaaatttac ttaataaata cttaataaaa ctgttctttt
    36541 ttagaaaatt tattttctaa ttattgaaca gttttatttt ttgtttaagt tatattcata
    36601 tgcaatttaa atagtttatt aataaatgaa aacttatttg tataatgact ttaataataa
    36661 ttagaaaaaa tgggcaactt aatagaataa ttggaggtgt aaatttataa aaaatgaata
    36721 ggataaataa aaaaatagat ttattaatat tggctttgct cgtttttata tttttaattg
    36781 ttggaatagt aatactatcg attaggacag aaaataaatt gaatgtgttc ataatgttag
    36841 caataatttt ttttataata atgttaactt atttatcaaa ttcagtagtg gggttgataa
    36901 caagttcaat aattatattt atgtatacat cctatatttt atataacaat attactcaca
    36961 atatggatgt tgaatttata tcatatatgt ggataatagc aacaccagta tcttcaatta
    37021 taatgggtaa tctgaacaaa agcataaatg aattgcaaaa tacaaataaa aaactatctg
    37081 aacagtataa ggaattagtg actattgata gtgaaactgg acttagaaat ttaaagattt
    37141 tttataatga tgtaaatatg gagataagta aatctattag acataatact gatttttctc
    37201 ttatgatagt aaaattacca tactacggaa acctacagac tatatttggt gaaaacaaaa
    37261 ctaataaaat agtaaaatat attggttcaa atattataga atgtacaaga aatgaagata
    37321 taatttatag tttacaaaaa gatatgatag ggatactaat gccaaataca tctttggaag
    37381 gttcaaaagt tgttaaagac agaataaaga aaaggataaa agaattaaat ttagatttaa
    37441 acaatagggg aaaatatgta aatatagatg tcaaaattgc atttttacaa tataagagta
    37501 gttttggaga tagtataaat tttaagaata tagttgaaga ggaacttcaa tatgatgttt
    37561 aaaatattta ataaaagtct attaaaatta gtagttgtat tatcaatagt actgacaata
    37621 tcaaatttta gcttatcttc atatgcatca gaaattactt ccagcacaga taaggtatta
    37681 atcatatatg attctaagaa agaaacagct tataatagag atattttaaa tattatgagg
    37741 actctattgg gaagatttag ttcagatata gagctattaa aattgagtaa ctatgatggt
    37801 gagataaata aaaattatta cagtcatata tttattttag gaatcaatga aaactcatat
    37861 aataatgata aaaacactaa aaacttgatt agttccttaa attcatataa aggaactata
    37921 tgttggttag gatatggtat agaaaatctt ttggaacata aaaaatacaa tttagactat
    37981 gtaggaaaaa caaacaatat agttagtgta aattatagag gtaaatcata taacttggat
    38041 gaacattatg tttttaacat tgtagaatca aaagatacaa gtaataaagt aataggaagt
    38101 attaatgata ccttaaataa gtatccatat ataataaatg ataaaaatct tttttatgta
    38161 tcaaaattag atttagatgg agttttattt tatatatttt gcgattcact taatgatatt
    38221 tttaatatta agacgtttga taaaggacgt atatttgtaa gaatagaaga tgtacatgct
    38281 tttagagaac caaaaaatct tgtagagata gcggattatt taagtagtaa gaatatacca
    38341 tttacgattg cattaatacc agcatatgta aatcctaaaa atcataaggt aataacttta
    38401 tcagaaagcc ctgaaattgt caaagcaata aaatatatgc aagataaagg tggaactgtg
    38461 atattgcatg gatatactca tcaatacaag aaagaagaag tttctggaga agggtatgaa
    38521 ttttgggatg gtaaaaaaga tgaaccctta aaagaaaata tgaagatatt tgtcaaagat
    38581 agagttttga atggtcttag agtatgtata gagaatggaa tttatcctct tgcatttgaa
    38641 gcgccacatt atgcaatgga atcagaggga tataaagaat taaaaaaata tttttctact
    38701 tatatagggc aacatcaaaa taatgataaa aaatttagca caaatacata tccttatatt
    38761 attagggata cagaggagtt taatatattt atacccgaaa atttaggata tatagaccca
    38821 gaagataagt ttacttttca aaatataaaa gaaaatttag ataaattatc cattgtaaga
    38881 ggttttagtg gagggttctt ctttcattca tatttaaaca tagagtatct aaaaaacaca
    38941 atagaatatt tagaaaaaca gaatatagaa tttatgaatt taagagattt taataattgg
    39001 gtaaaagttg atgaaataca aataagaaat aatggagatg aaattatagt caactatgat
    39061 aaggacttag atgaactaac taaaagtgat actagattta aatccatatc caatattagt
    39121 aaagttttaa tattcatagt atctataagt gttttaatat ttgtgattat atttatatat
    39181 tttaagagaa tagataaaaa gaaattttta aaataaagga ttttatatta gaatcaatat
    39241 tttaaaatat tttaggagaa atttatgaga gatttaaatg tatcagagta tttgttcgta
    39301 tttagtttgt ttagtatatg gtctttattg cttataaata ttattttagc tatgggtgga
    39361 tatatatttt attttaaaaa ctttgataaa gaaataaaag aaattgatga atatcctatg
    39421 atatcaatac ttgttcctgc tcataatgaa gctaaagtga taggtagaac tgtggagtct
    39481 ttgctattac tcaattatcc taagagtaag atggaactta ttgttataaa tgacaattct
    39541 tctgataata gtaaagaaat tttagaaaat ataaaggata ggtacaacaa ttataatttc
    39601 actataataa atacagatag tttaacaggt ggaaaaggaa agtctaatgc attaaatatt
    39661 ggatatacga tttcaaaagg agattttata gctgtttatg atgcagataa cacaccagat
    39721 aaaaatgcac ttagatatct agtacaaact atagtaatga atgatgaatt aggtgctgtt
    39781 attgggaaat ttaggactcg aaataaaaat aaaaacctat taaccaaatt tataaatata
    39841 gaaacattaa gctttcaatg gatgtcacaa gctggtagat ggcaactttt taatctatgt
    39901 acaataccag gaactaactt tatattgagg agaagtatca tagaagaaat tggaggatgg
    39961 gatagtaaag caattgcaga agatacagaa ataagtttta ggatatataa attgggatac
    40021 aagataaagc ttgtaccaca atctataaca tgggaacaag agccagaaac tgttaaggtg
    40081 tggataaagc aacgtactag atgggcaaaa gggaatatat atgtattaat gaaatatata
    40141 aaaaatatat ttaaacaggg aagaaataag attgtgtttg atatagctta ctttttttct
    40201 gtatattttc tatttttaac atctgtaatt atatccgata ttttatttgt tttaagtata
    40261 tctaaactag tagaaatttc tattccaatt aacttttttc taatatggat actatcttat
    40321 cttttattta ttatagaagt gagtataagc cttactatag aaaaaggtga agcaacaatt
    40381 gaaaatatat tcatagtggc aataatgtat tttacatact ctcaactatg gctgtttgta
    40441 gctataaaag gaatgataga gtatctaaaa gatataattt ttaaaagaga ggttaaatgg
    40501 tataaaacag aaagattttg attcatgtac aggaggaatt aacatgaaaa aatttattat
    40561 atcaattata tctttagtat tgtttttttc aaatatatca cttatataca aagtaaatgc
    40621 tgatgagact aaagttaaaa attataagtt tgaaagagat ataactattg atggtgttat
    40681 aggaagtaat agtacttttt ttgaagtaaa taaaaattgg gatattgaag aagttttatt
    40741 gcatcttaat tttagtaaga gccaaatatt aaatggagat gtttcaagtt taactgtatt
    40801 gataaataat gtaccaataa aatctattaa attaaatgct aagacaaatt ataaaaatac
    40861 attagaagta ttagttccaa aagattatat aattcaagga tataatgaaa taaaaataaa
    40921 aacatacaaa acaatatctg ataaaatttg ccaagatgat tccaatactg ggaactggat
    40981 ggttattcac aaagaatcat atatatcaat tagatataag cagaaaaagg tggaaaactc
    41041 aataaatgaa tatccgtatc catatgcaga aattgaaaat aatcataaat tagatacaac
    41101 tatagtagta ccagataata tgactagagg agagactaca gcagtgttta atttggcatc
    41161 agcatttgga aaaataacaa aaaatgatga tttaaagtta gatgttaaat tatattcaga
    41221 aatgaaaaat tggtcagatg ataatattat atatattggt aaacctgaaa atacagcaga
    41281 agaaattttg gatattttaa gtataaagga acaaacccta ttatcttcaa actgtataat
    41341 caagcaagta gattcaccat acaataagaa taagaagatg atggtggtaa ttggtagtaa
    41401 tgaagatgat ttaataaagg catcaaattt attaatagaa aatagacttt ctaaccaagt
    41461 tttatcatca tcagtacttg taaacaaaga gactaatata aaaattaata gggaacaaaa
    41521 attaaattta ggtcatttaa cattaaaaga tttaggatat tctgactttt tgttggaagg
    41581 agcatttaat caacaagctt tgtttgatgt caaaatacca acgggtaagg tattagatga
    41641 tggttcaaag attatcttaa atttaagata ttctgacaat ttagattttg aaaaatcttt
    41701 agttacagtg tctataaatg atgttattgt gggaagtaaa aaattggata ggtcacattc
    41761 taataatgat aagttagaat taaaaatacc taaagatata gataataaaa actattatca
    41821 agttaaattg acatttaatt tgagtataaa aaattcgaat tgtgttacta gagagagtaa
    41881 taatccttgg gcatatgtat caaataattc ttatttggcg ttatctacga aagaaaatga
    41941 aaccttatct tttgaaaatt atccgtatcc atttgttaga gatgatgaat ttaatgattt
    42001 aactgtgata atgccagatt attcagggtc acaagctatg acctggatgt ttagattagg
    42061 tgttacttta ggtgcgaata taaattcaca taatggaaat ataaatgtaa ttagaggaaa
    42121 agaatttagt gacaagtata aggatactaa tattgtagtt tttggagtgc cacataataa
    42181 ttctgttata aagatgttaa ataataattt aaatataaag ttcgataaaa attactccaa
    42241 ttttatatca aatgataaga taagctttat tgatgattat ggaaaaaata tttctactat
    42301 tcaattaata aaatcaccat ataataatca aaaaaatatc atggtaatta gttcaatgaa
    42361 tgaaaaaaac ttatatttag gaatggatta tttgttgaat aaaagtaaag ttaatgattt
    42421 aaaaggagat acattgataa ttgatgaata cggagaggta gaagatttag cttataattt
    42481 aaaatctaag aaagaagtaa aagattcaag ttggaatatg agtataaata agacaactaa
    42541 agtattttta atgatttctt ttattactat aattatagtc atgatattat caatgctata
    42601 tataaaaaaa tataaaagaa gataatatga taaatttatt attttaaaag aggtatataa
    42661 tttgaggaaa tatatgaatg aatttaaatt aaaaagaagt attttaatct cattaataat
    42721 cttaattttt atatttatag tttactttgt aagtattgag ttaaaagata ggagtctagt
    42781 aaataatcaa tataaaggta agattaaatc ggccaattta tctgtagatt atgaaataaa
    42841 tcaagttatg aaggatatag ataaattggg attgaataca gtaaatgtac ctataattat
    42901 aaatgtagag tccattaatt ctgatattat gtcaatagat aataatagta aagaaaaggc
    42961 aattaagtta ataaaaaaat taaataaaaa aggtatttca acaatattag aggcatatcc
    43021 atggattgaa aatggaaaat tgtatgagac tgactggaac cctataaata aaaaaagatt
    43081 tttttatact tggcaaaatg ccatattaaa tgaacttata attgatgtag caaatccact
    43141 ggatgtaaat gttttaaata ttggttctaa ttttgtacat ttggaagaat atcaacaaaa
    43201 ttggggtgaa ataatagatt ttgtacaatc caaatttgac ggtcttgtaa cgtacagaac
    43261 taattggtgg tatacaaaaa aagatgattt aagtagtaag ttgttttatg aacaaaagct
    43321 gaacaataat ttttttgata agttagattt tatatctata gcagcatatt ttgagttaag
    43381 taataaacct gttaatacag tggatgaatt aatttctgcc ttacactcat ctactgtaaa
    43441 caatagagga caaaatataa aacaagaaat atataacctt tataaagctc atagaaaacc
    43501 tatatttttt ggagaattag gtttttcaaa tagagaaagt gcaagttctc agccgtggaa
    43561 tcatactcct tcaaaagctg taaatggcga agaacaagca aggtgttttg aggcatataa
    43621 gaaagttttt gaaaatgaag actggataaa tggtttttct gtgttttgtg taggtaaaat
    43681 agatgacgaa aaaaattttt acccatcaaa agagagtata aaagttatta agtcttggta
    43741 tgaataattt tgaaagtgta gaatttatat agattaaata gatgattagg agagatataa
    43801 atgaatgaca tcaaggttat gactgtattt ggaactagac ctgaagctat caaagtagca
    43861 cctttaataa aggaacttga aaaaagagag aatataaaaa gtattgtttg cgttacagct
    43921 caacatagag aaatgcttga ccaagttata gaaaccttta atataaatgt agactatgat
    43981 ttggatataa tggaaaaagg acaaagcctg aatgatataa catgtaagat tttaaataaa
    44041 cttcccttga ttttaaacaa ggaaaatcca aatataatat tggtacatgg agatacaacc
    44101 acaactctag ctacaagttt gactgcattt tataataaaa ctcttgttgg tcatatagaa
    44161 gctgggctta gaacctatga taaatattct ccttttccag aggagctaaa tagacaactt
    44221 acaggtatta ttgctgatat gcactttgct cctactaatt tggctagaaa aaacttgatt
    44281 tctgaaggaa aacctaataa taatatattt gtaactggta atactgctat tgatgcttta
    44341 aagatgacaa taaaagaaaa ctataatcat cctataattg atgagattgg taacgatagg
    44401 atgattttgt taacctctca cagaagagaa aatttgggta aacctatgaa aaatatattc
    44461 agagctataa agcgaatagt agatgacttt gaagatgtac agatagtata tcccatacat
    44521 ttaaatccta aaatacgaac tatagctgat gagatatttg gaaaatttcc tgaaaaaatt
    44581 catattatag agcctttaga tgtagctgat tttcataact ttttaaataa atcttatatg
    44641 ataatgactg atagtggagg aatacaggaa gaagctccat cattaggtaa gccagttctt
    44701 gtgttgcgtg ataaaacaga aagaacagaa ggaattgaag caaagacact taaattagta
    44761 ggtactaatg aagatagaat atataattca gtgagcgatt tattaataaa taaagataac
    44821 tatgttcaga tgagtaaagc atctaatcca tatggagatg gaaatgcaag caaatatata
    44881 gtggatataa taattaaaaa atttaattgc aaatatttaa attagataag gcctaaaaca
    44941 ttaactaatt gatataagat ttatgataat attaagtaat acaattttta agggggagta
    45001 atatggctag aaaattaata aaaaatttgg gtaaaagtaa aagtgtaaag agagtgaaac
    45061 ttttatttaa gaagattttt attactgtat tcatagtagc aagtatagtt gctattttta
    45121 atataacgaa atattttgag gaattataca aagtaaggga tttaaaaagt actaaaattg
    45181 aatactatat ggatgtggct gatgaagcag gagatggaaa ggtccaatta agttggaagg
    45241 ccttacttgc tatagacatg gtaatacatg atgaagattt aagtaatata aaaaagaaag
    45301 acacattgga tataggggaa aagtttatag tagaagataa aaatggtaaa ggcgaaaagg
    45361 tgtataaggt aaaaaagttt aataaggtat taagcgaatt gaaatttgac tcttctcaaa
    45421 aaagtagagc aagaaaatac atgaaagatt tagaatacac ataccttgga aataaacaat
    45481 tagatagtag tgatgaaaaa attaaattta taaagaagtt agaagactca gctataagag
    45541 aatatattga ttatggaata ttgccctcta taacaattgg acaagctata ttagaatctg
    45601 gttggggaaa ttctaaactt acaaaacaga gtaataattt atttggtata aaagcagata
    45661 aagcatggaa aggaaaaagt gtagaaattt caacttcaga gcattataat gaaaaaattg
    45721 tagctagttt taggtcgtat aattcattac aagattctgt caaagaccac agtttatttt
    45781 taattaataa taaaagatat agaaaacatg ggttgtttga ggcaaaagat tatattagtc
    45841 aagctcaagc attagaaaat gcaggatata gtacagcaga agataaaaaa ggaaatcgta
    45901 tatatgcaga gctattaata gatgttatta ggagttataa tttgcaattg atagataata
    45961 aagttgagac aaaataattg ttaactttat atgaggagat aacaagataa gatttttttc
    46021 tacaaaaaga atagaaaaat tgtaaaaaaa actaaaaaaa tgtattgact tgttatttaa
    46081 aaggtgatat tattattact gtactcgaaa gagaacaaaa
//
LOCUS       DAEOSP010000008        44773 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.008, whole genome shotgun sequence.
ACCESSION   DAEOSP010000008 DAEOSP010000000
VERSION     DAEOSP010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.008"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..1269
                     /locus_tag="KRH54_000338"
     CDS             <1..1269
                     /locus_tag="KRH54_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8578017.1"
                     /translation="NNYIESVEVVNYIDNNNDISISRDISNFLESEYNITLTSAELYY
                     LVFQLTNKTTVLNYHEMDTKSLPNYIDEHFVKLTKKIIKNVYDLYLIDLSDEEFVVKF
                     TLHVKNLISRAKNNQVLRNQIPQKLKDSYPLIYDISVYICNQIQTLENVDIDEDEISY
                     ISLHVGSFFDRQKLLEDKVLCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHN
                     LDFDNKVDMVITTLPINNRCPIPFVYVNPYLNRKDYDNIQSKFTQIKDRKNILTVQNH
                     LEMYFSESLFMKNIYLDSAKDYIKFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNN
                     VAIPHSMKMDALKTGVCLIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEI
                     LSEWNNVKELTKADNYSSFMNRIAYLIQNI"
     gene            complement(1392..2546)
                     /locus_tag="KRH54_000339"
     CDS             complement(1392..2546)
                     /locus_tag="KRH54_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBG8578018.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDKNNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYEVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(2825..5440)
                     /gene="mngB"
                     /locus_tag="KRH54_000340"
     CDS             complement(2825..5440)
                     /gene="mngB"
                     /locus_tag="KRH54_000340"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG8578019.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVSTQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(5584..7509)
                     /gene="mngA"
                     /locus_tag="KRH54_000341"
     CDS             complement(5584..7509)
                     /gene="mngA"
                     /locus_tag="KRH54_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBG8578020.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYYEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEASFAAMTLETPIENWESVDPNNKVELI
                     FLIAVPENEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLTGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            complement(7639..8364)
                     /locus_tag="KRH54_000342"
     CDS             complement(7639..8364)
                     /locus_tag="KRH54_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG8578021.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(8627..9397)
                     /locus_tag="KRH54_000343"
     CDS             complement(8627..9397)
                     /locus_tag="KRH54_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBG8578022.1"
                     /translation="MKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPFSRQ
                     AIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGKEVF
                     NYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPYYLD
                     SCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSSLIQ
                     ERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(9399..10826)
                     /locus_tag="KRH54_000344"
     CDS             complement(9399..10826)
                     /locus_tag="KRH54_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG8578023.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGVRLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            11217..11939
                     /locus_tag="KRH54_000345"
     CDS             11217..11939
                     /locus_tag="KRH54_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8578024.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            12136..13803
                     /locus_tag="KRH54_000346"
     CDS             12136..13803
                     /locus_tag="KRH54_000346"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBG8578025.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(13899..15218)
                     /locus_tag="KRH54_000347"
     CDS             complement(13899..15218)
                     /locus_tag="KRH54_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG8578026.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQVAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGSPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(15259..16005)
                     /locus_tag="KRH54_000348"
     CDS             complement(15259..16005)
                     /locus_tag="KRH54_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBG8578027.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(16249..18258)
                     /locus_tag="KRH54_000349"
     CDS             complement(16249..18258)
                     /locus_tag="KRH54_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8578028.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(18652..20085)
                     /locus_tag="KRH54_000350"
     CDS             complement(18652..20085)
                     /locus_tag="KRH54_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG8578029.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(20172..22088)
                     /locus_tag="KRH54_000351"
     CDS             complement(20172..22088)
                     /locus_tag="KRH54_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG8578030.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLVAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQKLITVLA"
     gene            complement(22162..23022)
                     /locus_tag="KRH54_000352"
     CDS             complement(22162..23022)
                     /locus_tag="KRH54_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG8578031.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(23373..24560)
                     /locus_tag="KRH54_000353"
     CDS             complement(23373..24560)
                     /locus_tag="KRH54_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG8578032.1"
                     /translation="MIESIKKRAYEIENGLGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(24574..25965)
                     /locus_tag="KRH54_000354"
     CDS             complement(24574..25965)
                     /locus_tag="KRH54_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBG8578033.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQELMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            complement(26560..28260)
                     /locus_tag="KRH54_000355"
     CDS             complement(26560..28260)
                     /locus_tag="KRH54_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG8578034.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(28232..29635)
                     /locus_tag="KRH54_000356"
     CDS             complement(28232..29635)
                     /locus_tag="KRH54_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG8578035.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCNKIEAVFKTNDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(29899..30390)
                     /locus_tag="KRH54_000357"
     CDS             complement(29899..30390)
                     /locus_tag="KRH54_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG8578036.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(30416..31228)
                     /locus_tag="KRH54_000358"
     CDS             complement(30416..31228)
                     /locus_tag="KRH54_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG8578037.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEVMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKETLKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSEEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(31241..32392)
                     /locus_tag="KRH54_000359"
     CDS             complement(31241..32392)
                     /locus_tag="KRH54_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG8578038.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            32662..33303
                     /locus_tag="KRH54_000360"
     CDS             32662..33303
                     /locus_tag="KRH54_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBG8578039.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            33329..34873
                     /locus_tag="KRH54_000361"
     CDS             33329..34873
                     /locus_tag="KRH54_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG8578040.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(35073..35699)
                     /locus_tag="KRH54_000362"
     CDS             complement(35073..35699)
                     /locus_tag="KRH54_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG8578041.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(35834..36550)
                     /locus_tag="KRH54_000363"
     CDS             complement(35834..36550)
                     /locus_tag="KRH54_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBG8578042.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRSDNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            36976..38190
                     /locus_tag="KRH54_000364"
     CDS             36976..38190
                     /locus_tag="KRH54_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG8578043.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYKI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITNEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            38808..40136
                     /locus_tag="KRH54_000365"
     CDS             38808..40136
                     /locus_tag="KRH54_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578044.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCVGYGIGNDIKQAMSSAISANKESKINHLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(40264..41454)
                     /locus_tag="KRH54_000366"
     CDS             complement(40264..41454)
                     /locus_tag="KRH54_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8578045.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNIPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(41560..42939)
                     /locus_tag="KRH54_000367"
     CDS             complement(41560..42939)
                     /locus_tag="KRH54_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8578046.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(42970..44178)
                     /locus_tag="KRH54_000368"
     CDS             complement(42970..44178)
                     /locus_tag="KRH54_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578047.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLI
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
BASE COUNT        14788 a         7851 c         5087 g        17047 t
ORIGIN      
        1 aataactaca tagaatctgt cgaagtagta aactacatag ataataataa tgatataagt
       61 atttcaagag atatatcaaa ttttttagag tctgaataca atataacttt aactagtgca
      121 gaattatact acttagtatt tcagttaaca aacaaaacca cagtcttaaa ttatcatgaa
      181 atggatacta agtcattgcc aaattatata gatgaacatt ttgttaaatt aactaaaaaa
      241 ataattaaaa atgtatatga cttatatctt atagatttat ctgatgagga atttgttgta
      301 aaatttactc ttcatgtaaa aaatttaatc agtagagcca aaaataatca agttttaaga
      361 aatcaaatac ctcaaaagtt aaaagattcc taccctttga tatatgatat atctgtttat
      421 atttgcaatc agattcaaac tttagaaaat gtagatatag acgaagatga aatatcttat
      481 atatctcttc atgtaggttc attctttgat agacaaaaat tattagaaga taaagttcta
      541 tgtgcattaa ttactccaaa ttactatgat ttacaattca aaatagtaag agacctcgag
      601 aagagattta atgaatccat agaaattatt cagatttttt cagatactca taatcttgat
      661 tttgacaata aggtagacat ggttattact actttaccaa ttaacaatag atgccctata
      721 ccttttgttt atgtaaatcc ttacctaaat agaaaggatt atgataatat tcaaagtaaa
      781 tttactcaaa ttaaagatag aaaaaatata cttactgtac aaaatcattt agaaatgtac
      841 tttagtgaat ctttgtttat gaaaaatata tatttagata gtgctaaaga ttatataaaa
      901 tttatgggaa atattctgta cgaaaataaa tatgtcaaac ccaattatat agatgatgtt
      961 cttataagag aaaaaatgtc ttcaactgca tttaataaca atgtagcaat tcctcatagc
     1021 atgaaaatgg atgctttaaa aactggagta tgtttaattg ttaacgataa accagttaaa
     1081 tggggtgagg aaaaagttca gattatagct atgataccta taaatgaaaa agaaaaagaa
     1141 aaattcaatt acatatttga aagctttatt gaaatattat ctgagtggaa taatgttaaa
     1201 gaacttacta aggcagataa ctatagtagt tttatgaata gaatagctta tcttattcaa
     1261 aatatctaaa tcaattaatt tatttccaat atatgtttct taagttatta acataaaata
     1321 gataagctgt cacactaaaa agtctaaatt aatatgtgac agctttttac ctaatatacc
     1381 ttgttttaat actacatatt ttttgactgt tgtatactag ttataagatt gaatatttgt
     1441 gttgttgtag ctgtcatatt ctcactagca gttgctttat ccattgcttc ttctattgtc
     1501 atagctctat ttactataga aaaataagca tctataccct cttcattaca tttaactgca
     1561 tcttctgttg tggctccagc caaacctatt acttttacct catatttttt tgccagtttt
     1621 gcaactccta ttggagcctt gcccatagca gtttgattgt ccaatctacc ttcaccagtt
     1681 atgacaaaat ctgcatcttt taactcttcc tctaatttaa tttcttctaa tattattttt
     1741 attccagatt ctaattttga atttaaaaat gctaaaaaag caaaacctaa tcctcctgct
     1801 gctcctgctc cttctacatt agctacatct ttacttaagt aatttttaac tactttggaa
     1861 aaatttctaa gcccattatc taactcctca attatttctg atgttgcacc tttttgagac
     1921 ccgtatatgt atgcagctcc atttttccca aacagcggat tatttacatc acaggctatt
     1981 ttaaacttac attcatctaa ttcctttaat ctattttcta tttttatatg tcttatttca
     2041 tttaaaactt ttccacctaa tcctactaac ttgttatttt tatcataaaa ctcaaaccct
     2101 aaggcttgaa gcatacctat cccagcatca ttagtagcac ttccacctat cccaactata
     2161 aaatttctat agcctttatt tattgcttcc tttattattt ccccaacacc ataagttgta
     2221 gtgtataaag gatttctaag ttcagtcgga accaatgtta atcctgatgc ttgagctatt
     2281 tctattattg ctgtacttgt attttttaat aatccatatg ttgcatccac ttttttagct
     2341 attggtccag taacttctat agtctctttt ttaccattca tcccttgaac taaagcatct
     2401 actgtccctt caccaccatc agcaagtggc aatatcttta cttgcgcatc ttctttaacc
     2461 ttcaatatcc ccttttttat tgcatttcct gcctctattg aagataagct tcctttcaaa
     2521 gaatctatag aaattaatat tttcatactt atctactcct ttttattact ccttatttta
     2581 aacatataat tttataagct taatttatct tttattttat actctacatc tctctttgta
     2641 tacaacttat accattaata ttcttttatc ttataaaaag ggagtgtctc aaaataaatt
     2701 tcttagttca tgagacactc ttttgtataa aatcaaatat atgtttatta tttcaacaat
     2761 gataaggagg attatctcta ttttaagata attccttttt agcactatct aatcattttt
     2821 agcactattt aaccagtata gttttaactt ggttcattaa gcactctaca tttacagagt
     2881 tattatctat tgatatagat tctaattctt tttcatttag gttaatccat ttagctgttt
     2941 taatattttt gttaaatact atgttagctg attttgtttc ttctttagaa ttataaaatc
     3001 taactactaa tgcctcttct ttttcagcct tttttacaac acttaacaca aggtccttat
     3061 tagtttcctt aagtaaacta tatgaataat ctacttctat ttctgattga ttcaatttca
     3121 tagcattata tggcatttta ttataagttt gtatttttgt taaatactct ttagccatat
     3181 ttgcaacatt tgcttctagt gtagattttt catgagtaac tattgctaag tctattttta
     3241 tatctcctat catttgagaa tctggagtag gcaactttat tcctgatgga cgacctgggc
     3301 gtcttgccat ttcttcttta cctaggaatc ctacacttct aaataatgtt atagctattg
     3361 tgtcaaattt ttcccctact atttcaaact ctcttgtact gtttgtaaga acagctactc
     3421 catgctcttt gtcagacaat ccaacgaatg ttagcattgg atatatagaa tctggtcttt
     3481 cactccattc ttcctgttgc caaacttcca tagcatcatc atacacactt ctctttatat
     3541 gtccaaactg attatcagac acagaaaact ttgaagctat atttgttggt atataagttc
     3601 taaccctaca atctcttgct tggttattta tgttgaatac tacatctatt atagctttat
     3661 catttggaac atttataact atatcaaagt caataaatga atctgctcta ttattttttc
     3721 tagcttgtat atcttttggt acttttaatc tataatttat ctttatctca ttattaaatc
     3781 tatttttctt tatagtggaa tttgctacta tattatcgtt aaatattaat ttctcatttg
     3841 gaagtggtga aaaatcatac tcatctcctt catctcctcc attttccatc aataatacat
     3901 tttcaaaagt tttatttaat tttttatcaa atatctttaa tgtcccgttt tcattaactt
     3961 gaatagtata aaagtcatta tctaccatgt cacatacttc ataagctttt tctaacatat
     4021 gttcaacttc ttttatcata tacgccttat accccattgc aggtatttta tctttaaagt
     4081 ttattgtata taccataaat gaatcatagt ttccataatg aacaatttga cggtctatta
     4141 atcctgcatc tattacttct ttatgtacca cttcaaaatc caacttatta ttatctttat
     4201 caaatatttc aaatgctttc attttagtta taacttgggt gctcactatt tcattcctat
     4261 cgtaaggcat taagttaaat gctactaact tatctaaagc catttcacaa ctcattgcat
     4321 caactattct tcttttgtaa aatgttatta attggtctac tttttcctca gctaagaaaa
     4381 atctattcat tatttcctgg tgaactttat ctgaacaaca acatccaatg ctgtcatgag
     4441 catgattttt cattatttct ttccaaatta actctattaa tccatgatga tattcaaatc
     4501 ctagagaata tgcaatagaa gctagtggct ccaatatgtt agttatcttt gattctattc
     4561 tagcatttgc tgattttata tccattcttg ttgagaatat acttctatga accctcatat
     4621 atttcccatc taaaaattcc ccttttattg tatctatatc ctcatttttt tctagctctt
     4681 taaatatgtt ttcatatcta cttaagaaaa attttctctc tggataacat tcttttaatt
     4741 tatatataac ttcaaatata ttcttttgaa ttggcatttg gtcatgtcca tttggaagta
     4801 ttatatggtc agtagttgca cccttatcaa gaaccggtaa atatttatcc attcttgttc
     4861 ttagctcatc ttcattcatt ggtagatatt ttcctattgc ataacctaat ggaagtattt
     4921 gaaccaaaac agaagaccca tcatcactct tccaattaaa ctctgtttta ttagttcctt
     4981 ttctttcaga acatcctctc caaaacattg agtattttat atcaaaccca tttagtattt
     5041 gtggcatttg agcactttga ccaaatgaat ctggtaaata tcctattttc atatattctc
     5101 caaattcatc acaatctttt attccatata gtaaatttct aagtatggat tctccaccaa
     5161 ctaccatttc atcagtttga gtataccaag gacctattat tagccttcct tcttgaacta
     5221 atttttttat tctctcctta cattcaggct taactgctaa ataatcttct aatatagcag
     5281 tttgaccatc catcacataa tatggatagt tatgattatt ttctaacata tccattattt
     5341 cttccatatt atttacaagt aatattctag attcctcagt tgagaagtac cattctctat
     5401 cccaatgcat atgaggaact atgtgtacat tttttttcat tgcttatcct ccaaaactta
     5461 tctgataata ttttctgtct aattaaaaat ccaataaaaa tactactgag tttacaaaat
     5521 tttttaatat ttattttatc actataatta aacctttgat ttaaatatct aataacgtat
     5581 cttttaatgt atctttcttt gatttttttg tttcctagtt attattaata atattgctga
     5641 tattatagct ccaagtactg ccgcaaagaa ccatattgat gctataataa atcctgacat
     5701 acttccctct aaaagcgcca atgagaatat ccccgctcca ggcacttgaa gacctatatt
     5761 gaagaatgct actatagacc cagttatggc tgaaccagct accaatgatg gtataacacg
     5821 gattgggtcc tctatcataa atggtattgc accttcagtt attccagcca atcctaatat
     5881 ccaagtttgc tttcctattt ccttttctga ttctgaaaat aaatctctgc ctaaaacagt
     5941 agctattgta actccaaaag ctgaaaccat cttaactgat gcaaatattg cataaggtat
     6001 aaaatttcca cttgctattg ctcctattgc aaatgtatag gctgctttat ttattggtcc
     6061 acctaagtca aatgaaacca tagctcctat tattgcacct agtagtattg catttactcc
     6121 agtcatccca tttaaccaat ttataagacc attgttaagt gctgctacag gctttcctac
     6181 tacaaataac atcaataaac ctactaccaa agaaccaact actggatata cccaaaaact
     6241 tacgaatcca gctaatgtgc ctttggctgg tatattcttc tttaaaaatt ttactatata
     6301 gccagtaagt aaacctccta acatacctcc aagaaatcca ctacctatta aatcagctgc
     6361 tagacctgct gcaaatcctg gtccaagacc tggtttttca gctagtgagt aagacatata
     6421 tgctgataat actggaacca tcaatgtacc taacatacct ccagaaagct ttcttaattg
     6481 ccataaccaa gaaccttctt ggttgtatag ttcttgtaga ccaaatgctt gagatgctaa
     6541 tactgcaaat gcaagtatca tacctcctgc cacaataagt ggtattatat gagatactcc
     6601 tgtaagtatt gctgatttta catctttttt ccatgatgat ttttcacaat atgatatatc
     6661 agatgatttt tctatataat cacctttacc ttgtttttgt gctacttcta aagcttcgta
     6721 tattatttct tttgctcttt ttagtggagc tgctactgac gtttttatgt ttggcaaatg
     6781 ttcaaatctt tctgtatttt taggtgctac atctgttgca tatattattg catctgccct
     6841 ttttattata tctggtgtat gagggtccac cataccatta gctccttgct tttctacata
     6901 cacatccaca ccgatttctt taccagcttt aattaatgct tctgctgaca tatatgtatg
     6961 tgctatacct gcagggcatg ctgttattgc taatataatt ttttttgatt cattttgaat
     7021 cctaatctct tcttttattt gttctttctt ttcttttata tctaagttat taaataactc
     7081 tttcttagtt tttgaattta acaacatatt catataatct tcatttgaca gtctagtaac
     7141 taactcagaa agtatttgta tgtgtgtatt tccaccttca ttttctggta ctgctattaa
     7201 aaatattagc tctactttat tatttgggtc tacgctttcc caattttcta ttggtgtctc
     7261 taaggtcata gctgcaaatg aagcttcttt aactgactta gattttccat gaggaattgc
     7321 aagccctctt tcaaaacctg ttggagataa agtttctctg tacattacag cttctaaata
     7381 ttcctcataa gatgttaact tgccttcata atatagcttt cttgctaaga actttattgc
     7441 gtcttccttt gtttttattt tttgttttaa aaaaacaagg ttttcatttg tgattttctt
     7501 aaattccaaa attacgccct ccttaaatca tttaatgcta tgaatatagc ataaaataaa
     7561 ttatgaaaat gtttttttga aattatacac acaatttctt cttaaaaaat aaagatttgt
     7621 gtatttatac acaaatctct atcttaacca gtattgctct attattgctc tcataatcat
     7681 cataattgga accttatctg atatattgta cccatttact tgtttaattg ttgaatagca
     7741 atataaactt atatctgcaa tttgttgcaa ttgattttca catatatgtg ttagagctac
     7801 taccttacaa cccttattaa ctgctgtctt tgctgcttct ataacttctt tcgtttctcc
     7861 acttaaacta agtgcaaaaa acatatcttg ttcacctaat ttccctatat catgaagtac
     7921 ttcatgtctt tgactatagt aagttatatt atcatgccca cctctaattt cttttactaa
     7981 tatctcacat aaaggaatgt tttgacctat tccataaact ataatactcc tagatttttg
     8041 aattatatct gctactttat ttattttttc ataatctatt agctctaatg tcttttttat
     8101 atctgattca aaaccatttt ttggctcttt atcctcattt tttaactctt cttttaaatt
     8161 attttttagt tgagagaaac catcatactc cattttttta catagcctaa ctatggtatt
     8221 tgggacagta aaaaatttat ctgctaaaga ttgtattgat aattttacta tttcttcttt
     8281 gttttttctc atatattcaa ttatcatgtc ttctgtatct gtaaatttat actcatattt
     8341 ttttgctctt tcataaaatt ccatagtttc tcctccatct attttattat tcataagtaa
     8401 aattcaaaaa tattagctaa aattttcgtt aatataatta taatttaata tttttaattt
     8461 attaatgcat actactcttt ttattataca taaaaaatgt gtacttccta tattgataga
     8521 gatgattcaa gtttttattt tgatacatct ctatcaatat ttttttagaa aatacacatt
     8581 tttttcaaat acttattttt aattaaaact ttaggtaatc tattctttat atataattat
     8641 tagacttgaa aatatcttta aaatcaacca attcaatatt atttcttttt aaccacattt
     8701 taacttcctt atcacatagc attgcatgtt catatactct ctcttgaata agagaagatg
     8761 attttataag actataatct ataaatcctg gatgagtaac catcaatgtt atatctttat
     8821 ctaaatttat atatttttct tcaaataatt taacaaaaga tttatttaac ttgtaaaaat
     8881 cattctgagg atatttactt ggcatgctaa tacatgaatc caaataatag ggacaacttg
     8941 gtataccgta ttcttttgca gcatcacaca ttgctgatat tgatacatct tcttctatac
     9001 catgtatatc aatatactca ggcaatttac tcatatacaa cttgtaagtt tcaatttgag
     9061 cacaaacttc ttcatatact tcttcataat taaaaacctc tttacctgtc agtagttgcg
     9121 ttcttcttat tttgctagaa acaaattttc caccttcatc taacaaattc tttatcttaa
     9181 tatgttcttt gcaacaaggt tctcctatta ctaaattaac atgtaaacca aaacatacat
     9241 catcattttt taccatttct atagcttgtc ttgaaaaagg catattcacc atgattccag
     9301 cacttgtaac aataccgttc ttataagaag atattatccc tagtgtaaca ccttctgaat
     9361 atcctatatc atctgcccta actactagtt tcttcatttt atgcgtctgc ctgctttctc
     9421 aactcatcaa gttgcatttc catctcatcc tttactttaa caacatccgt tcctatgatg
     9481 atttgaactg atgtattact caatttaatt acccctttgg ctcctgtttt tttaatatta
     9541 tcatcattca aaatagtagc atccttcact tctaaacgta atctcgtcat acaattatta
     9601 agagtcacta tattttcata accaccacaa tttttaatta tctgtttagc catatatgaa
     9661 aaattaccat gagaaagttt taaattcttt tctttttcac taactttatc acctatctct
     9721 atctcaactt cacgtcctgg tgtttgcaca ttatctttta ttattatata tttaaatgtt
     9781 acataatata atacaaagaa tacaactcct acaggtataa ctaaccacgc tttatctcca
     9841 taactaaaac ttaagacata gtcaattata cttgctccat taaatgaacc caagcgaaca
     9901 cccagtaaat atagtacaac tccacctaga ccagaataaa aagaatgaat tatatataac
     9961 tttggagatg caaataaaaa cgaaaattct attggttcag ttactgttgc cataaaagaa
    10021 gtcaatgctc cagcggtcat tgcacctttt acttcttgct tctttttatc ttgtgctgtt
    10081 ttatatatag cataagaaat agcaggtatt gaaaacatca taattatgta aaatcctgtc
    10141 cagaagaatc ctgcatttgg gtctcctgcc ataaatctag gaatttctcc tcttataact
    10201 tctcctgttg atgttgtata ttcacccatt tcaaataata tgtaagcatt taaaacttga
    10261 tgtaacccag ttggtattag tgcacggtta cagaataaaa acaagaatat tcctattgca
    10321 ccagctcctc ccaaccaaaa accaaattga ttcattgcag cttgtggata tatccataat
    10381 aaactaaata tatatgataa aattaaagca accactggtg atagtgtaat tacacatttt
    10441 tctcctgcaa agaagcttaa aaatgctgga actttaactt tatatgtttt attgtaagtc
    10501 catgctatac aagctccaac aattatccca ccaaatacac ctgcatttaa tgtatcattc
    10561 aataactcta aatttgactt aaatatcata tatgcaacta cggctgcaac cattacactt
    10621 gtcttgtctt tagactttga gtaggcacta gctgctccta ataaaaataa gtagtccaca
    10681 tttgaaaata ctatattaga tatattagat atagctggca cattgagcat atttggacca
    10741 aataataatc ctaatatacc tgctgctggc attacagcta ttggcactag catagtcttt
    10801 cctaaaagtg acagttgttg catcattttg tttaagaacc tcttcatatc caatcccctt
    10861 tctatttcgg tttaattttt tgtgaaaacg atttgttcac attttatatt ttaaatatta
    10921 tatttgttta aacaaattgt caatattttt tattcatttt atttttatca tttaatttaa
    10981 catataaatc cttttaagtg tattgctata tctagtctat ttttttaatt catttttttt
    11041 attttaaaaa tatgtttaac catgtatttg aaacaaccca caaaataatt ctattttgtc
    11101 ctttttatgg ttgatatgat tctattttca ctatacatgt ctaaacacat ttgatataat
    11161 ttgttcatat cagttcaata atattactaa agacctttaa ggagggatag gggtatatgg
    11221 ctaaaaataa attttcagag atatatgaag tattgaaaga ggaaatactt gatggtaaat
    11281 atacaagtaa tatgatgctg cctactgaac ttcaacttat tgaaaggttt tcatgtagca
    11341 gaaacactgt acgtagagca atttctcaat taaacacaga aggatatgtt caaagtataa
    11401 aaggcaaggg tgttgtagtg ttagaaaata gttgttctaa tgattttttt ctaaatatgc
    11461 acaatttcaa aggtgtagaa tcgattgtag aagataaaaa ggttaatact gctactagtg
    11521 ttctccactt cagcaaaatc ttaattgata ataagttatc taaaaagaca ggttttaaag
    11581 ttggtagtga agtatattac cttcacagac taagatatat tgataacata cctaaaattc
    11641 tcgacattaa ctattttcta tgtagtatag ttaaagacct tgatgtttct atagcacaag
    11701 gctctattta taaatatata gaagagtgtc taggtacaaa aattgtttca tccagaaaga
    11761 tatttaaaat tgaaaaagct acagagcttg agttaaaaac gctacctcta aatgattata
    11821 attgtgttgg tgttattaaa aatagtgtat atactgatga tggaaagctt tttgaatata
    11881 ctgaatcaaa gcatacacct gaaacatttg tattcatgga tgttactcaa agatattaaa
    11941 aaaattatgt ttaattaaac ataatttttt tacgtttaga cactttatcc catactaacc
    12001 tcactattct atatacaatt atatgtttat acaaataatt atttattgta tattttagct
    12061 ttaatattag tttaacgctt gacaatttgt ttaaacatat tataattaac ttataaatta
    12121 caggaggtat caagtatgaa aaattggtgg aaaaaagcaa ctgtctatca aatatatcca
    12181 aaaagtttca aagatagcaa caatgatggt ataggtgata taaatggtat tatagaaaaa
    12241 ttagattatc tgtattcttt aggtgttgat ttattatggt tgactcctat gtacgtttct
    12301 cctcaaagag ataatggata tgatatagaa gattattata atatagaccc aaaatatggg
    12361 acaatgagtg actttgaaaa actcttaaaa gaagctcata aaagagacat aaaaataatg
    12421 atggatatgg tcctaaacca tacatctact gaacataaat ggtttaaaga atctaaaaaa
    12481 agcaaagaca atccatatcg tgattattat ttctggaaag atgctaaacc tgatggttct
    12541 gttcctaata actggatatc cagatttagt ggaactgctt ggaaatatga tgaaaccact
    12601 aaccaatatt atttacattt atttgaagaa actcaagctg atttaaattg ggaaaatgaa
    12661 aaggttcgtg aagaatgtta caaaatacta gaattttggg ctgataaagg tattgatggt
    12721 tttcgtttag atgtagtcaa cctactctcc aaaacacctg gattacctga tgaccctata
    12781 acaggaccaa aaggagatgg aagaactcac tacgctgatg gtcctcgtat acacgaatat
    12841 ttacataata tgaatcaaaa agttttcaaa cctaaaaata tagttactgt aggtgaaatg
    12901 tcatctacta cacctgaaga gtgtatcaac tatactagag aaaatcgaga agaactatct
    12961 atggttttta gcttccatca tatgaaaaca gattataaag gaggcgcaaa atggactaat
    13021 gaaatgttct ctctggataa attaaaaaaa gcacaatctg actttcaata taaaatgtat
    13081 gaaggaaaag gatggaatgc tctattttac tctaatcacg accaacctag agctttatct
    13141 agatttggag atgatagatt ttttcgtcaa gaaagtgcta agatgttagc cataacatta
    13201 tttggtctac agggtactac atacatatat caaggtgaag aaataggtat gacaaatgcc
    13261 tactttacta aaatagatga atatgatgat gctgaaagta agaatgcata ctatcatatg
    13321 attaaatctg gtattgatga aaaggaggca ttgcttatac ttcaacaaaa atccagagat
    13381 aatgcccgta ctcctatgca atgggataat actatttata aggggttttc taatcataaa
    13441 ccgtggttaa aagttaataa tgacaatatt agtgtagaaa atgagcttaa tgattctcgt
    13501 tcagtatttt atacttatca aagattaatc aaatatcgta aacaatatga tatttttaca
    13561 gaaggtactt atagattgtt agatgaaaat cataaaaatc tatttgttta tgaaagagag
    13621 tataaaaacc aaaatcttct tgttataagt aattttactc atgataatgt agtatataaa
    13681 ttacctgaaa cttatttgaa taaattaaac tacacaatac ttgaaaccaa ttacactaga
    13741 gatataattg aaaataaaat ggaaataaaa ccttacgaaa gtataatgat atattataaa
    13801 taaagttatt tatgtaaaaa tcggtataac accctggtgt tataccaatt ttactaaatt
    13861 caaagtaata tttatagaat atctatagat tacaataatt atttgacttc tacatcatta
    13921 tatttctttg aatatacttt agtcatatag aaatatatag gaactacaat tatcatccca
    13981 cctaatcccc aaactatctt cataggttca tcaatacctg cttttatcag taataatata
    14041 cttataataa ctgcaaatat tggtataatt gctcctccag gtattctaaa atttacatct
    14101 ttaccagcat acttctttct caatattagt acagctaaac atgtaggaat atattgaata
    14161 aatctagata caacacttat ttgagataat gttgtgaatg aacccgacca tgctattagt
    14221 aaagttccta tgacagatac tataattgct atataaggag atccattttt attagttttt
    14281 cttatagact taggcattaa tccttgttca accaaagcag cagcagacct tggtgtaaat
    14341 atggatgatg caatatttat acctccaaca gatactatag ttcctattgt aattatatac
    14401 ccacctatag gacctaacat cttaatggca gcatctgcaa caggagtagc tgaccctgct
    14461 aagccattac ccaacacccc tatacttatt cccagtataa gcatatatat tactgatact
    14521 acaaacatta ccattaccaa tgctttaggt acatttttct tagggttctc catatctttt
    14581 gctgcaacag ctaaagattc aaatcctgta aaagcataaa ataaagttat agatactgct
    14641 acaaaagctg atgacgcact ttgacctttt tgtattatta caaatggggt aaaattagaa
    14701 ccctttataa agaataatcc tacagctata aataaaacta ttggtaataa ttttcctata
    14761 gtcatgatat tatttaagtg cttagattgt ttcattccca taaaatttat aatcccaagc
    14821 cctacaacta atataccaat tattatattt ttaataagtg gttctgctac ttgtggccat
    14881 acagatgata gtgttacagc aaagaaattt gccatagtag cccaagcaat catactaact
    14941 atccacttca taaatccaac ttcaaatcca acaaaatctc caaatgcctc ttttgcataa
    15001 agatagggcc ctccatcagt atcaaaccaa ctacctgctt cagcaaaaca taaagcaata
    15061 gaaacagcta aaagcataca aaaaactaat actaatatac ttgctggacc tatatctgta
    15121 taagcatcac caggtaaccc gaatatacct gaccctatta ttgcattgat tcctaatagt
    15181 acaatactcc aaaaacctaa cttaatgcct ttttccattg ttattccccc caaagccaac
    15241 ttaatatatt tatatttttt atttagatgc ttctttaact attgccataa atattttttt
    15301 catattatca taatctctag tcatcatttc tggatgccat tgtatcccta ttacaaactc
    15361 acttccttcc ttttctatag cttcaattaa accgtcttta gatgtagctg aaacaatata
    15421 tcccggtgct actttgttga caactaagtg atgaaaacta ttaactaaaa cttctttttc
    15481 tcctagtatt tcatataatt ttgtcccctc ctttatctgt actgtatgtg taggtacatt
    15541 agatagatgt tgctgattgt gtttaatata agcaccttca attaaagata aatcttgata
    15601 caaactacct ccttcagtta catttataat ttgttctcct ctacatatac ctaaaactgg
    15661 ttttttcata tctaaagcat gttttaataa tttcatatca aaactatctc tctttggtga
    15721 tattgccccc aacttattat gaggttcttc tttccatatg agtggattta catcttgtcc
    15781 cccagatagt ataagcgcat ctatattaga tacctgctcc tttataatct catcatcata
    15841 tacaatcggt acaatatatg gtatagcttt acacataact actgattgta tataatcatt
    15901 atttacataa gccctttcat agccaggaaa cattcctcct tcatcaatta gtaaattacc
    15961 tgatattcct attattggat atttcttttt agccaatttc ttcacccctt aacttatttg
    16021 tgaacaataa aaaatgctac ttctcttaaa gagcaagtag cataccaaga ttttatttat
    16081 ttgaacatta taatttattt tagaaatact ttttttgaat acgagtaata taaataactt
    16141 tatttactta tattttattt taatcctgtt tagtttgact tttttaaatt gttttaagta
    16201 caactaatag gtaaaatagg catttagatt ttccttttta tcaacctatt aaatactgtt
    16261 taatatattt tttccccttc tatttaattc acttcccttt ctaccagatt cacatattat
    16321 caaatccagc tcttttaaca tattaagtat tcctcttacc tcaccttctg ttatattgta
    16381 gcctaattca tttattgaat taacaatacc atttctacct atatttttat taagattatt
    16441 ggcatttttt aaacactcca ttactaactt tactttctct atagatgttc tatcctttaa
    16501 tatgcttatt tctttactaa aatcttcagt tatatttaat aagttatgag gtaaatcatt
    16561 tattaaaata acatcctcag aactcataat gttaatatac atagcagtat tttttaattc
    16621 tcttatatta cctggccatt catatgcatt aataatgtct tgaacatctt gactaattgc
    16681 tctcttcctt ttcataaaat gctttaacat aacttctata tcttcttttc gctctcttaa
    16741 aggtggtata tttatgggca atacatttat cctatagtat aaatctgctc taaatctatc
    16801 tccttttatc atttctaata aatttttatt tgtagccgca attactctaa catctatatc
    16861 tattacattt tctcctccaa ccctcattac ttgattctct tgtaatactc taagtaattt
    16921 gctttgtaag tatataggca tgtctcctat ttcatccaaa aatatagttc cattgttagc
    16981 ctgttcaaat aatccttttt tacctccttt taaagctcct gtaaaagcac cttcttcata
    17041 tccaaaaagt tcactttcaa gtaaattttc tggcatagct gcacagttta ttgctataaa
    17101 tggctgacta tgtcttggtg atgcattatg aatagactga gccatgagtt cttttcctgt
    17161 tccactttct cccgttataa gaattgtata atcagattca gctattttca tacctaattt
    17221 cttagttctt tccatcttcg ttgaaactgt ttttatatca tcaaaagtat atctagcaac
    17281 ttgtcccttt tctctaagct tctttgatag gttttgctcc aattttctta tgtaagttat
    17341 ttcttgaata caaaaataat atccagtaac ccttccaaaa ctctctatat tatacttatt
    17401 tacgtttaag tacttattat taaattttat aacttcatca tgtgccttac tcaaactaaa
    17461 aatcttttta atattctcat tctctaattc atctatattt ttccctataa tattactttg
    17521 aatatctagt atatccctta aactattatt acaaataatt atgtttccct tatctgatac
    17581 cattgccata cctaagtttg ataaatttat tatggaattt agttcatctg cttttatagt
    17641 aagttctttg tatttagtat ttattcctga atataaacta attatttcat ctgtatactt
    17701 aattagcctc tcagtaattt ctttatcatc cagtttgagt tttgctatta tttgaataaa
    17761 cgttgaacta tcaatatacc tatggcctaa atcaataact tcttctatat attttggaac
    17821 taaacttgac tctcctggcg ttatagctat cttaatattt ctatattctt cattgggatt
    17881 gtatggtata aaattcaaat gatttattcc tatattgtat aaattagata ttgtttccaa
    17941 tacagtatgc ttattatcgt ttactactaa tacatctatt ccttctggca atgaaaataa
    18001 cttatatata tccttttgct ttatgctcct atttatagtt attatctttg agacatcttc
    18061 cacatatttt tttattttta atactctatc atctatcatt accaatatta tgtcatcttt
    18121 tattaattcc ttctctgtta ttttatttaa ataataattt tttatcatta ctttatcttt
    18181 aaagatcatt tctagatttt tatttaagaa acattctaat ttagacaacc catcagttac
    18241 tactgctatt gttttcattt gattactcct tttaaaatat actctactct tttataatat
    18301 acatttgatt ttcactttaa taacacaaaa aattgtacat ttagtatact aacatattcc
    18361 tttattagtt ttatgtccat aaatatcata gttgcaatat ctatcatatt tcttaaactt
    18421 ctataaagca aaaattgtgc caaattaata attttcttaa ttttattcta aagttcctca
    18481 atagtattta cagacttagc acttatagct tataagtctt tatattatta atctattttt
    18541 ataggttaat tgtacaaatc atatatcact tttacctatt aaatgcatta taaaaaattg
    18601 tatttataaa cactatttac tactcttcta agtacaaaca gtatctacat actataacct
    18661 ttcaccatta gattctatta cttgtttata ccagtcaaaa ctctttttct taatcctaga
    18721 caagtctcca cttccatcat catacttatc cacgtatata aacccatatc tcttggccat
    18781 ctcaccagta gatgcactta ctaaatctat acatccccat ggagtatatc caaataaatc
    18841 aacgccatct tctacagctt ccttcatttg ctctatatgt gcttttaaat actctatacg
    18901 atagtcatca tttatcactc cattagattc ttttttatca taagctccta aaccattttc
    18961 aactaccatc ataggtattc tatatctatc ataaatttca tttagtgcat atctaagccc
    19021 ttcagggtct atttgccatc cccattcact agacttaaga tatggatttt tcactccacc
    19081 taatatgttg ccacttgtat tatcttcttt agaatcacta cttacacatg tagacatata
    19141 ataactaaat gtataaaaat ctacacaacc ttcttttaaa atttctaaat ctcctttttc
    19201 catttttatt tcaatattat tttctccaaa atatctattc atataagttg gatattcgcc
    19261 acgcacttgt acatctccac agaaataatt cattactcta ctatctcttt gagttttaac
    19321 tgcatcatct ggattacaag tataaggata cattgttgca aatatttgca tacatccaac
    19381 ttgataattt gaatctattt catgagctaa cttaacagcc ttagcacttg ctacaaattg
    19441 atgatgtaac ccttgaaaac gaagttgagg tatatctact tggtttataa aatctgtagt
    19501 cccttcattt aatattcctt gtgataagta gcctcccata ggcattgtag ctgaatttat
    19561 ttcattaaat gtcagccaat attttacttt tcctttatat cttttaaata ttgtttcaca
    19621 gtaattcata tacaagtcta ttacatctct tgaaacccat ccattgtatt tctctgttat
    19681 tgcaaatggt atttcatagt gagatattgt tattaatggt tctatattat attttaaaca
    19741 ttcatctatc acattttcat aaaatttaag tcctgcttca tttggttctt tctccattcc
    19801 agttggaaat attctagtcc atgctattga aaatctaaat accttaaatc ccatttctgc
    19861 aaataaagct atatcttctt tgtatctgtg ataaaaatct attgcttcat gacttggata
    19921 aaatgttcct ttttctataa ctctcgttat tcttttactt actttttgag aacctccagt
    19981 acacatatct gaacaagaag gacctttccc atctaaattc catgctcctt cacattgatt
    20041 tgctgctgtt gctccacccc ataaaaaatc ttttctaagt gccataattc tacctccaat
    20101 aatttattta tagtaacttt attttaaaat aaaaatacat atatataatt cttaattaat
    20161 agaactctct tttacgctaa aacagttatc aatttctgac tattatttac atgcttttgt
    20221 gtattttcta caatatccac ataatcagaa taattagtaa caactactgg tgtttctaag
    20281 caatatcctt cttttgaaat actttctatg tcaaaagtta ttaatgtctg acctttttta
    20341 atcttatccc cttgattaac aaaagatttg aaatatttac cttccaactg aatagtattt
    20401 aaccctatat gtatcaatag ttcacaacca ttatcagata taattcctat tgcatgtttt
    20461 gttggaaata aagtcattac agtcccatca aatggtgcca ctacttttcc ttctttaggt
    20521 gttattgcta caccttttcc caatatacct gttgaaaacg ctgcatcttg tatctgctcc
    20581 aaagccttta cttcaccttt tattgggcta tatactactt ctcttttgtt tttttctata
    20641 acgccttgcc cattatcatc tttagaaatt tctactatag catcatcttt ccaaaaaaat
    20701 aatgttaaaa caaatcctac tacaatagct actattattg ctattatagt tgcataaaca
    20761 cctttcatat ctccagttgt tggatttata taattaggta taccaaatat tccaagtcca
    20821 cccattacat aactttttac attcattatc cccattacag ctcctgccac agcaccacct
    20881 acacaactat atatgaatgg tgtttttctt ggtaaagtta ttccatatat tgcaggctct
    20941 gttacaccac atatccctga aataactgca ggtatacaca gctcttttat cttcttatct
    21001 tttaatttaa agtacattgc tgctactact gctgtttgtg caaaagatgg tacaaatgct
    21061 cccactaata ttgtgtcaaa ccccatagtc ataaggtttg acatagctat tggtactaat
    21121 ccccaatgta gaccaaatat tacaagcact tgccacaata aaccagttaa tgcaccacat
    21181 aaaattggtg aaaagttaaa taaagctaaa aatccatttc caactatatt agaagcatag
    21241 gtagctatag gccctataac cataaatccc aatggtaaag aaactaataa aactgccatt
    21301 ggtacaaaga aattctttac tacatctggt attattttag caaacacttt ctccaactta
    21361 gctactaata atactattaa aattactggt actactgttg aagtgtagtt cattgctatt
    21421 actggtaatc ctaagaaagt tatatatact ggtgattcaa atattgttcc agtaaataga
    21481 ttgtataaag gttgacctgt agatgctaga gtatctaatt gaatacctga atagcataaa
    21541 actgcaccta taagcattcc aataaatggt tttaatttga atttcttagc agctgtatat
    21601 ccaagaatta ttggcatata ctggaacatt gcatctccaa ttgcatttaa cataatatat
    21661 gcaccatcag tttctccata caaacccaat gctgtaaata ctgcattaaa tccttttatc
    21721 ataccagcag cacacagaac tcctaatatt ggttgaaaaa ctcctgatat tatgtctata
    21781 aacttattaa gtaatccttt tggcgcatct gagtctgaat tatctattgt atctcctcct
    21841 aatccagcca aatcgcacac aacttcataa acatctggaa catgattacc aataactact
    21901 tggtattgac ctgcactttt cattacagta actataccat ccattttttt tagcacatca
    21961 tcatttgcta ttgactcatc ttttaattta aaacgtagcc tcgttataca gtgagataaa
    22021 ctgctaatat tatcctttcc tcctacattc gcaatgattt cttttgatag ttgttcatac
    22081 tttctcattt tttctcctcc attttctttt tgaaatatga aggtttggcc aacttaatgt
    22141 caccacccaa tatatataaa cctaattatt aggatttata tctgacttaa tacttttact
    22201 taccactctt tcaacgtgaa tagttaagta taatttttcc tcatctgtta aatcgtagtt
    22261 gtatttttta tgtataaatt catccaactt ttctatgaat ttataagcat ttttatattt
    22321 aacttttatt acatttaata aatcatcttc attattttca tgtaaattat tactaacaat
    22381 tcgttctgca aaatacttta agtgagtaat aaatctatga taataaactg aatcttcatc
    22441 aaatactatt ctgtaatggt atttaaccat atttgaaatt tcttgtatta tttgtgtaat
    22501 ctcatatata gtttttatac tttgatccat ttgtgcatta actatatgca gtgctataaa
    22561 tccagcttca tcttctgata gtctaatttc aaattttttt tctatataat ccaatgcctc
    22621 taaccctatt ttaaattcac tcttataaaa tctttttata tcccaaagta atacattttt
    22681 tacacttact ccttcacgag ctctttctat tgcagtatac atatggtctg ttaaagaaat
    22741 ataaatacta tcatttaatc ttttccctag tttttcttta gcatataaaa ttatttcatc
    22801 tgataattcc atatatctta taggaatatc tgctattagt tctttaaatt tatcagatat
    22861 atttgggtct gaaagtttga atatcttatc aattttatcc tttgatatat gctctcctgt
    22921 acgtttttta taagctaaac ctctccccat aactactatt tccttatgat tttcatcagt
    22981 tgtaataatt acattattat tgagtatttt ttcaataatc attgtgatgc cccctttatt
    23041 agcattaagt taaaataaat cttttttaat tatttagaat atttaaaatg taatttatta
    23101 cataaaaaaa aacctattta ttacacatta aatagactcc catgagtcct gagtaataaa
    23161 taggtttagc ttgtatttaa acaatcacta tccgtttgta ttttatttta attattattt
    23221 attatatgtt agcaaattta ttttttagtg tcaacttttt ctcaatattt taattttaaa
    23281 taaattatat aaattgaatc ttcaaagtaa tatttttatc aataaacaaa ataaaaaagg
    23341 tattactttc tttaagtaat acccatttat ttttaattta agtggttttt cttaatctca
    23401 tcaagtagtc catcttctaa ataaatttct aatgcacatc ctaccattgc ctttactgct
    23461 ttgtgaagtt tatcataagc ttcctcagaa tttgcataat ttagaaattc tttttcatga
    23521 acatatacca ttggacttat atctaaggct atttctgtat acatagtagg gcaaacttga
    23581 cttacattac ctatatctgt agaacctact ggaccatctt taccttctaa tatatctgtt
    23641 atacctgcct caatcaaatt attctttgtt atcttttgaa gtactttgtt atttattatg
    23701 ttgtcaaaag aattttcaaa gtatctataa gaaagcttag ctcctgtcat taactctgca
    23761 ccttttgcac agtttataac ttttttagtt acttcatcta aataacttct ttcagcagct
    23821 cttacgaaaa acttacaggc tgctttttct ggaactatat ttggagcctc tcctccattt
    23881 gaaactatac catgtattct aacatctgat tttacatgtt gtcttagtgc atttatccca
    23941 gcaaaagtaa gttgtactgc atctagtgca ttaactccca tatgagggtg actagctgca
    24001 tgtgctgata ccccttcaaa actaaactct aatgaatcta tcgccaaagc cttacagttt
    24061 atattattaa aagcttctat atgcatttga taacttacat caacatcatc aaaagctcct
    24121 gccttaacta aatcacattt tcctcctgtt gtttcttcag ctggtgttcc tattagtaca
    24181 atttttcctt taaaattttc ttttaactta cttaatacta tacaagctcc tagtgtagat
    24241 gctgcaatcc agttatgtcc acaagcatga gctggttctt tattttctcc atatccagga
    24301 agtgcatcat actcagctaa aaatgctatg gtaggtccat cattatctcc taattcagct
    24361 ctaaaagcag tctccatatt acaatatgga taaactgtat caaatccata ttccttcatt
    24421 ttttctacta agtattttga tgatatgtat tcttcttccc ctaattcagg attgttaaat
    24481 ataaaattac aaacttcttc aatttctttt ccaagtccat tctctatctc ataagctctc
    24541 ttctttatac tctctatcat aatttacctc ctactagaat cctagatata atcctattgt
    24601 caaaaatgcc attacaagaa ttgttaatac taccattaaa ggaattacga atctaaccca
    24661 ctttgaccaa ggaatattgg ctactgctag tgctcccatt aaaacacctg atgtaggtgt
    24721 tatcaagtta gttacaccta taccaaattg ataagctgta actatcattt gtcttgatac
    24781 atctactaag tcgccaagtg gtgcaagtac aggtattgta agtgatgcaa gccctgatgc
    24841 tgatggtatt aaaaatgcta ttaatccttc tattaagaaa gttaaattta taaatatagt
    24901 ttttggtaat ccatttaata aatttgctgc agaatttaaa attgtatcta ttataaatcc
    24961 attttctgct attattacga ttccacgtgc aaatgctata cacaatcctg ctgtaattaa
    25021 atctcctgct ccacttataa atgaattaac tatctcatct tgtttaagcc ctgatgctat
    25081 tcctgatatt atacctatag ctgtaaatat cattgcaatt tcagttatgt accattctag
    25141 tcttaaaact ccatatatca taatcgccat acctattgtg aaaatggcta ttaccatggc
    25201 ttctcttttt gtaaattcct ttatatttgt atcactatta accattaatt cttgatttag
    25261 tgatatatca tgtactaaag attttttagg gtctttttta actttattgg catacatcat
    25321 aacaaatcct atagatatag ccatatatat tataaacata acaactctaa actctattcc
    25381 agaacctggt actatttgtg ataatgcttg tgctattcct acagagaaag gattcgtagt
    25441 agctgctcca aatccagctg ttgctcctat aaatactgtg gccactgctg tcaatgaatc
    25501 atatcccata ctggtcataa gtggaataaa tatcatataa aaaggtagtg tctcttcact
    25561 cattccaaac gtcgtcccac ctagtccaaa tagaatcatt gaaatcggta ttattaaaaa
    25621 ttgaaacttc ttcattttat ttacagtatg agctataaca gcttctatag ctcctgtttt
    25681 attaactatc ccaaaagctc ctccaactat taaaacaaat ccaactactt cagctgcatc
    25741 tataaacccg tttataggtg ctgtaaatac atcagtaatt ccttgtggat ttgatggtgc
    25801 tttttcataa gttccagcta caacaactga ccttccgtct atatctactc tttcaaaatt
    25861 tccacttggc actatccaag taagaattgc cattaacact attagcaaaa atattattgt
    25921 aagtgtctgt ggcatagtaa actttttctt ttttaaactg ctcattttta atctccccac
    25981 tttttaaatt aaatatttta ttattgcatc cctttatcct attttgtata gatgtaaaaa
    26041 agctcaacat ggatacatgt tgagctaaat gtgctaaata caacatacct gcatcttccc
    26101 tttatcttgt ttaagatagc tctccatcaa aattcgggat aattttgatg acagtactgt
    26161 acctattaaa tacagtccca gcactagctt cggacactag cacttcggca cttactcctt
    26221 ttatattgcc tcattggttt ccaacctctt caatattaat cttagtaaat gcaacctcta
    26281 cctcaatctg tttgttagat gaggtaattc ttattaattt ttattaattc aaagtaattg
    26341 tagcataata tgtcgagatt gtaaatattt tcagtataat aattcttact ttatgatttc
    26401 attccatttt tcacaaataa ttttttctcc atataattta aatactgtag tatattttaa
    26461 atacacacaa actttccaca aattttaaat tattataata cctttataat aaaaaataaa
    26521 agctatgtaa acttatcaat ttacatagct ttatatacat taatctactc caagtacagc
    26581 tttaagcttt gatgctattg taataactcg tggaccaaat ataacttgaa ctgagttttt
    26641 ctttttaaca actccacttg cacccaattc atttacccag aagccatctt catagaatcc
    26701 atcaattgaa tttagctcta ccctaagtct agttgcacag tttgtaacat tttttatatt
    26761 attacttcct cctaaacctt ctattataga atgtacaagc tttgtatctt tatcatctac
    26821 ttcctcttct actgcattgt tgtatgctgt tttaccagca tctacagatt gtctttctct
    26881 atattctttc ttactataaa gcttaacttc ttctgagctt cttcctggtg tttttatatc
    26941 aaacttcttg attgcccatg taaatattat ataagtaact ataaaaaata ttggtatcaa
    27001 aaataataat ggtaacgcat ccactttttg tggttgtaat aaattaggaa acatatttct
    27061 aaatgattct cccattatag aaacattaaa catttctgta aatacatacg tcaatcctgc
    27121 cagtggtaca tatgctacaa aatataataa tggtgcaaca aataggaatg tatactctat
    27181 tggttccgtt acccctacca acatggctgt tactattgct ggtatcaata tacttattgc
    27241 ttttttctta ttagcattat cagccaactt atacatagct aaagctgctg ctggtaatat
    27301 tgcaaaatgt attaataaac gacctgttgt gaagttacga gttatataag atagtgtatc
    27361 tgggtctccc atttgagcta atcttatatt attaacaccc tcgtaaattg ctccacctac
    27421 ttccattgtt ccacctactc ttgtatattc tatagggaat gcaattaagt gatgtatacc
    27481 aaatggtaat aatgctttat ccattgctcc aaaagtaaat gttcctaata atccactaga
    27541 acctatccaa ttaccaagtt tagataatgc caaccctact ggtggccata taaagtacat
    27601 tgctgtacct aatactattg atgctataaa catcataatc attgaaaatc ttggacctga
    27661 gaaaaaagat aatgcactag gaagttctat gtcaacaaat ttgttatgta ctgcactagc
    27721 tacaaatcca gctattatag ctccaaatat actacaatca tatgaaaaga ttcctaagaa
    27781 agaaccatat aaagaacttc gactatatgc ttctgcctct cctaatccag atgccataaa
    27841 cgcttctgct gtaactgtat ctgaatttat accatttgct gctaacaatg tacttataac
    27901 tgtgtgcatt cctataaata aaacaattcc tcctaatgca ccccatcctt tttccttttt
    27961 agataatcca aaggctatac ctgtaacaaa gaatattggt aagtatctca ttaccataaa
    28021 tcctaaatcg tttattactt taaagaatac atttataaat gacccttctt ttattagtcc
    28081 gctaagccct aatgcagaaa cgttttccat atttgcaaaa gctgacccta atcctataaa
    28141 tactcctgct acaacaagta atataacagg tatcatcatt gctccagaaa gtacttgtaa
    28201 tttatccttc atagtattat ttcctccatt tttattgaaa tgccaggcta tataatgcat
    28261 atatatatac ttctattgct ttgtataatg tatctatact tatattttca tttgcctgat
    28321 gtgaatttgg attatccccc tctattgtgg gtccaaatgc aactccattt tttaacactc
    28381 ttgcataaga tgcagctcct tttgtaaaaa gttcagcttc ttttccaaaa ccttgtttat
    28441 atgcatttga taatttattt attaattcac tatctggttt tacatacagt aattttaaat
    28501 ccttatatat atcaactatt aaattttctt tttttccaat ttcattaatt ctattagtta
    28561 taaactccca acttataccc ttaggatatc taaagtctat tttcatattc aattcatttt
    28621 tctctaagaa gatactcatt atacatattg tagaaacccc catatctaca tcttctttat
    28681 atatccctaa tttttttcca tgattatcat ctgtaaagta tgaatttaaa atatccctta
    28741 ttataacgcc tttatcattt agttttttaa ttttctctaa atcttttgct aaactaaatg
    28801 cacagttata tgacctgtgt ggatttctcg ataaagccct ttctcctgta tatcttacta
    28861 atactttacc ttcttctagt tcttctatct ctgtataata agctaatgat tctactaaat
    28921 ctttcttatc attagtttta aaaacagcct caattttgtt acatacaaat ccatcttctt
    28981 tgtttgactt tatatcaact aaattgtgat ttctaaattt atctttatct attttctttc
    29041 ttagattgaa gtataaaata cctttttctc catttactat tgggaagttt ccatctggag
    29101 aaaatgcaaa ttttggctgt tcattgacac taaagtaatg ctccatacat tcccaagtcg
    29161 tctcttcagc tccacccact atcaatctaa tctttctctt cggcttttca ttaagttctc
    29221 ttaagaaaag taatgcatat aaaatcccta tcaaaggtcc tttgttgtca ttaactcctc
    29281 taccataaag atagttatct ttttgacata ctttaaaagg tttactcttc caaagttctt
    29341 tattatatat tggcaccaca tctatatgat taagcacccc tactacttct tctccttctc
    29401 catattctat atgaattgca tatccatcaa agtcttttac aacaaaatct ttgtcttttg
    29461 ctatttgaat aagcttatcg aaggcatttc ttatttctat cccaaatggt tgatttatat
    29521 ctgttgtact ttcatctcta attgaaggta tttcaactaa acttctaata tcatttatga
    29581 tattttcttg attggatttt aaataatcaa taattagttc tttgtttact atcatcttaa
    29641 ttatttatca cccctttata ttaattttac aaagcatttc agttcatatc tacattataa
    29701 attttgaata ttattttttc aatatttcaa atacttctct aagttaccat aatgatacgg
    29761 taaacaatca acagattatg acaaaatcat actataccta ttactaatat tttaacttat
    29821 ctattacgtc ttttttaatg taagtttatt ccaaaaatac ataaaaaaag atgtagcctt
    29881 agctacatct tcttatatct attcaacttc cattattatg ctattaatat ctacatttcc
    29941 aggttctatc ctatctttta ctgttctctt aacagaatca gcatcatcca ttataactac
    30001 aggagttatt atatttaaat catttgacgt tataaaatct aaatctactt ccattattaa
    30061 atctttagct tttaccttat ctcctgcctt taccttaaca tcaaacggct ttccttctag
    30121 tgacattgtc tctaaaccta catgtattaa tatatttgta ttatcattac tcttaatcat
    30181 tattgcatgc tttgtatcaa atacattgac tatttcacca tcaacaggag aatataactt
    30241 accttcactt ggtattattg ctactccatc tcccatcatc ttttgggcaa aaactgcatc
    30301 tggaacatct tctaagttca ctaaatttcc cttaaaaggg ctatatattt tctcaatctt
    30361 attttctttt ttcttaaata tattaaacat aacacactct ccacattata aaattttatg
    30421 ataatatttt tcttaaagct tttgctacac catcatttac attagtatca gttacaaaat
    30481 ctgccataga ttttacttca tctgtagcat ttcccatagc aataccaaga cctgcaaatt
    30541 taatcatgga tatatcattt tcattatcac ctatacaaat aacttcttcg ctatctatac
    30601 ctaatatatc acaaaatttt ttaactgcaa taccttttga agtacctttg ctcataactt
    30661 caaaatttat ctttccagaa cttactactt ctaaatcttc ttgtttttta aattcttcct
    30721 ttgcttctct caaagaatct acgttttctg agaaaagtat acattttaaa atttcattat
    30781 cagctttttc taataaagtt tcacaacttg cattttctat tatttcaatt ctgtcttctt
    30841 ctttattttt actatttatt aatctatatg gatgttcttc acctatttca ttgttggata
    30901 taactatttt acagccttta aaatgtttgt ttaaattata cttttcacct attgtataga
    30961 ttttctttag agtttctttg ctcaaaacct tcttatactc atatcccagt aatttaaaat
    31021 atgtcccatt agttgatata atatatatat catcccctaa aacacttgta aaatatttca
    31081 tagcattata aggtcttccc gtaactaagg ctaccttgat acccttttcc atgacttctt
    31141 ttagtgcttt aatattttct tctgatactt caaatccttt acccatcaaa gtaccatcca
    31201 tatcagtaca aattaattta tatcgcattt gtatcacatc ctagtaagat ttttctaata
    31261 tttcaacaag gtcttctttc tttccaaaag gtaaaaatcc taaaactgga cagcttgtta
    31321 tttgttctaa ttgttccttt gttacattaa actcagacat ttttgtctta agtcccactc
    31381 tctttaaaaa actttctatt tcatttccta aagttttagc tgcttcatca tcttccactt
    31441 tttctaattc ttggtttagc attctcgcta ctttggcaaa tttctctata ttatccttat
    31501 agaacttttc tgcaaatttt gggaacacta tagccaaagc ctcaccatga ggaatatgtg
    31561 taactcctcc tattatttca cttagtggat gtggtgctgc tgctccagca tttgccagtg
    31621 aacttcctcc taatgtatct gctactgcta actttgttct gtattctata ttatttttat
    31681 cttccattac tttaggtaga tagttaatga ctaattccat agatttttca gacattatct
    31741 ctgtaaaatc attagcattt ggatttatat aactttcaaa tgcatgggta aaagcatcaa
    31801 atgctgttga tgcagtaatt ctctctggca gagttaacat aagctctggg tcaactatac
    31861 attcttttga gaaacaattt tgatgaaata ttgtattttt ctcgcttcct ctcgatatta
    31921 ctgctgcttg tgtaacttgg cttcctgttc cagaagtagt tgatacagct attaacggta
    31981 attctttttc acttacctta tcgtatattt taaatggatt tgtatatgta ctaaacatat
    32041 aatcccaatc tatcttttct aaaccaaaag tcaatgctaa tacttttgca gtatctatac
    32101 ttgaacctcc tccaacagct aaaacaacat ctacagattc ttttttagct aattcaaacc
    32161 cttcatttat taattctaca gttgggtttg gctctactct atcaaagtgt acaacttcta
    32221 tacctttttc tattaaattc aatgtaactc ttttatataa cttgtctaat ggctctacat
    32281 ttggagtagt aactaacaaa catttctttc catattttcc tattattcta ggtaattcac
    32341 ttaaagttcc ttgtccaaaa tttattcttg atggttgata aaagttaatc atatttttcc
    32401 tcccttaaat tattgccttc atattcaaat tatacatatc ataggatttt taaggcaata
    32461 ccttcactgt taactcttat ttactaaagc ttatggcaat tattagacaa taaatgtttt
    32521 attattacca tatcattatg taaattttat taagtaccca tgttataatt ttataatcaa
    32581 tcttattgta taattgactt aataataaaa atagagtata tacacttttt tatgacaaat
    32641 agcttaaagg agaattaaat tatgaaatat ttgatagaca gtgctaatct tgatgaaatt
    32701 caaaagtgtt taaagtatgg atttaaggga ataacagcta atccatctat gtatcttaaa
    32761 gagaaagtaa acttccatga atttattgag acatgtgcta atttaaatcc agaaatatta
    32821 actgctgaag tcattggaaa taatttatac gaattaatta attcatgtga taagatatta
    32881 aatataaata aaaacactat aataaaaata aatttctctg aagttggctt agaacttata
    32941 aacatattaa atagtaaagg tgtaaaaact gcatgtacct tgattttcaa tgtaaatcaa
    33001 gcatcattag ctataaatgc aggtgctgat tacctattcc cttttatagg tagaaatgat
    33061 gaaaatggga atgatggtat aaaaaacctt acagaaatat gtaaccttat atcaataaat
    33121 ggatataata ctaaagttgt tgcagcttct attaaaaatg tattccactt aactcaagca
    33181 tctctttgtg gatgtgatta tgcagctgta acatatgatt tatttaaaaa agcttctttt
    33241 aaccagctta caatagatgg tgccaatact tttgaaagag attggtccaa ggttaataaa
    33301 tagtaactaa gatttaggag ggtttgcatt gaacaaaaga ttattagata ttgctgaatt
    33361 attactcaac agctcagact atattactgt agatactatt gccgaagaat taaaagttag
    33421 taataaaact attcgtaatg accttgttat tttagatgaa tggcttttag agtttaattt
    33481 atccctagat aaaaaaactg gttcaggggt tattattcta ggtaatgaag atataaaact
    33541 taaagttatt agagatatca atgataaatc aaattgtata tatgcatact ctcctgaaga
    33601 tagaaaaagg tatattctaa gtaaattatt tattaaaaat tctaagttta ggattcgtga
    33661 tatttgcaat gaactacatg ttagtagagc tactgtgcat aaagacttgg tagttattca
    33721 aaattttttg caaaacttta aagtgactct agttagaaaa accaataatg gagtatattt
    33781 agagggaaaa gagaaagata ttcgaaaagc aatttttgaa cttgctactg ttaataaaag
    33841 ttatactgaa cttaaagaaa tattattctc gaaatctaat gaatgcaaag ataccccatc
    33901 aacaaaaata tttaaggagc tatttaacta tgattttgag aaattatctc aaatcactct
    33961 acacacctta caagtagaaa aaagtaacct tagtgatgag tattatataa acttcttaat
    34021 acatatagct atatgtatta aaagaatttc aattaaaaga tatattaact tatctgaagg
    34081 tttttttaga gaattattag aacatgaata ctttgaaaaa tcaaaaattt tatgtgctaa
    34141 cctaagtgaa gcttttgacg tagaatttat tgatgaagag gtctgttaca tccttcttca
    34201 tatagaagga ttacttaaat caataaagaa aactactact cttgaacctc ttaaaaatga
    34261 agaaattaca gagtataatc tatctgaagc aatagctgtt cattggggaa gtatactaaa
    34321 tttaaactta aaggacgatt taatattaat aaagtctctt gctaatcacc ttaaatcagt
    34381 tctacacaga ataaactatg ggtttactat tacaaatccc atattaaatg atataaagag
    34441 aacatttcct tatacttata aggctgctaa agagtctaat actgttatca aacagttgat
    34501 gaattatgaa gtcaatgatg acgaaatagg atatcttgct ttatatttaa tatcagcaat
    34561 agatagaagc aaatctcccc taaatactat tttaatatgt cactgtagtg atggtgtatc
    34621 taatttatta gtccaaaagt taagttttga atttaatcag attaatatag ttaaatctat
    34681 acctctatct tctatttcat ttacgaactt tgatgatgtg gacttaatac taactactgc
    34741 tcctgtagac tttgaacata atgctgaact gataaatata aatgctcttt taagtaagaa
    34801 tgatatttct agacttagta caattataaa aaaactatat tctgaaaaaa ataaaatatt
    34861 atcttataaa taaacaaaaa taggcttgtc tcaacataga gataaaccta tttttcattg
    34921 tccaaatgat aaaaatatat ttgatttcat agaaaaagtg cctcatgaac taaaaaattc
    34981 attttgagac accttcattt tttatattta atctttttat ttaagctttt atatttttta
    35041 aacttttata tttaaagtaa attttattat ttttattgag cacaagcatt atttatatta
    35101 ttaatttctt cagtattctt accaaacttt gtagttacaa atcctattgt ttttattatg
    35161 agtaataagg atatcaatga atatatagat atcataaaat tcttacttat tattccagac
    35221 gctactatta taaatccatc tagcataaat aatataactg gcaacttcag aaacttaatt
    35281 accttattaa taagcattgc aactaaatca gttccaccag tactacagcc ccttattatt
    35341 gctatcccta tagcagttcc taccaataac ccacctatca ctcctgctac taatagattg
    35401 tcagttatgt gagtttgtgg tattactttt aagaataatg ggcccataag agttatatat
    35461 aatgttttta ttccaaaacc tttccctaaa aataataatc ccattactaa aagtggcata
    35521 gatattccta atgatatatt ctcaacaggc attgataaga atgtgctaag tacgactgat
    35581 aatcctgtta tacctcctgg agctaatgta tgttctgcaa agaaaaaatt aactgctaat
    35641 gccattaaaa atgctgatat tgttatcagt aaataatctt ttatcatttc ttttttcatt
    35701 taataacctc ctatgattat attgtttcag tataaaaaat caattaaatt agtctagctt
    35761 cctgtagttt ttcataaaat taatgcttcc ttaaaacatt aagaaagcat ctcttttatc
    35821 atagtttatt atcttattct aaaccaaact ttgtagctgc ttcatctata gcttttctac
    35881 aattcttaac acctgctgtt ataccatctt tatccttgaa tattgctgaa gataatatta
    35941 caacgtctgg gttacattct attaatggct ccatattgtc tgatcttagt ccaccatcta
    36001 ttgctaattc acacttagga ttcttttcgt ctatcatttt tctagctctt tttactaaat
    36061 caacagcact ctttctccat ccccagttat cttttccatc agtttcatca acaccatgaa
    36121 cgactatttg caatctgtct atatcatata tagcttcatc aacaaaacat aatggtgtgt
    36181 aacatccaat tgttaatcct actttcatac caaattctct acagtaattt attatgtatg
    36241 caagaggagc acctaaaaaa tgttctgctg gtattatcaa caagttacat cctgctgctg
    36301 ctattttttc tataaataat ctatcacaat ctcttgtgta tatatggcat tctattggtt
    36361 tatctgtcac tggtcttatt gcttctatta tttgatgacc acccataagt tgcatatttt
    36421 ttaagtcatg catatctgct gcatctgaat gtacgtagtc tacacctgca tccattgctt
    36481 ctttaactac ggctgatata tgaccgtaat ttacatgtgc taatcctgct gctattttta
    36541 tgtgtttcat atttgtatac ctccatattt tactttaatt agtattaaat attagttttt
    36601 gatatttttt taatattttt taatccagta ttacttttat ttagcattac ttttattgct
    36661 gtatatattt cttttatgaa cttattctgc ttttgttaac tgtgaactta ttttgctttg
    36721 taaattaata ttacactgat atattttttt tcacaacact ttgactactc cttaaaagta
    36781 cctttatatt gtggtaaatt atagacatta attgtatata aaaagttagt tttttaccac
    36841 attcttatgg catttattgt ctatactaac tcaattgata tagctagttc atttaatata
    36901 taatcagact ataaatataa atttactcga taaaaagtac cattcataac aatattaatg
    36961 aaagggagat gtaaaatgag aaactgtatt attgcccaat ctggaggccc tacagctgtt
    37021 atcaatgctt ctgcagttgg tatttatgaa aaaaatttag aaactaaata ctttgataaa
    37081 gtatattttg gtatcaatgg gattgaaggt atattaaaca aaaatataat agattcagac
    37141 tccttaaaca aagataatgt attaaattta aagcaaacac cttcgtctgg tcttggttct
    37201 tgtagataca aactggctga ttataaaata aattcttatg attatgatag agttataaat
    37261 atactagatg aatataaaat agatacatta ttttatatag gcggaaatga ttctatggac
    37321 acagtgatga aattttctca atactgcaaa aacactactt gtaaaataaa atttataggt
    37381 attcctaaaa caatagataa tgatttatta ggaactgaca actgtcctgg atttggttct
    37441 gctgcaaagc ttatttctac actaataatg gaaacatact tagatttttc tatatacaaa
    37501 aataatggta tatttatagt agaaactatg ggtagagata ctggatggtt agcagcttct
    37561 tcttcactag ctagacttaa tgatactcca cttgttgatt taatatacct tccagaaatt
    37621 gatttctatg aagataagtt tatagaagat gttagaaaaa tatttaaaga aaaaaataag
    37681 gcttatatag ttgcatcaga aggtataagg gataaatacg gcaactttat atgcgaacaa
    37741 aaaacttctg ctcatgataa atttggtcat tcccaattag gtggagtaag caactatcta
    37801 aagaatctta tagtatcatc atcaattaca acaagagtaa aaaccttaga acttggtgta
    37861 atacaaagat gtgctatgca tttagcttca gatactgatt taaaacaagc agctgaagtt
    37921 ggtgcatatg ggatagaact atctaaaaaa ggatatacag gagttatggc tatcattaat
    37981 agaacatgca ataatcctta taagtatgaa gttagtcata ctgatatttc taaaatagct
    38041 aacaagacaa aatattttcc taaagagtgg ataaatgaag aatgtaatta tataactaat
    38101 gaagccctaa attatctata tccacttatt caaggtaaac cagatatcaa atataaaaat
    38161 ggactacctt atttttcaat tatttactag gaaattatta taatttgata tacactcaat
    38221 tctatcgctc ttaaatacct aagtggctgt taaataaaat tataaattgt tatatgacag
    38281 tcacttaatc gtttaattat ttaactgttt agttttttaa ttttttaatc tttttaatac
    38341 taatacaatt tgttgtaata atatctcaca cccattttca ttttggatag attataatta
    38401 gttagacaat aaatattttg tcatgatatt attatatata gtgatacaaa tagaaatact
    38461 tttatcatgg ctagaaaaat aaagattaca caaaggattt aaaagtcaaa caacttcaat
    38521 taaaataaat ggttaaaata acttctaaat caactacata aacaataaaa gaacataaag
    38581 tagttaaatc tacaattata aaattagtaa gactataata acttaaataa aattaaagtt
    38641 aacaaagact attaaagtta gcaaatattc ctctacctaa ataattgctt tttataaaga
    38701 gttatttatt ttaattgaat ttaattgaaa tacgatttta actagaacaa tagattaatg
    38761 taatatttaa tataaaataa ttataataat tgaaaaggaa gtaatctatg atgaaaatag
    38821 cagtcataac aaccgaattt ttaaaagagt ttgtagataa ttctataaaa aagttaaata
    38881 ttaatgcaga aattgaaata tatatctata gagatttttc tcatgttgga gatttatatt
    38941 tagaaataga agataggttt gatggatttg cagttagtgg ccctatcccc aaaaaagcta
    39001 ttacaaaaaa ggctggaact attaaaaaac ctctagttga ttttggtact gacttacaaa
    39061 gttactatga tatgtttcta aagcttatct ttaaatataa tacattagat ttttctagag
    39121 catactttga cttaacagac tgggcagata atccaaaaga tatatcatat tatttatcaa
    39181 atggcacatt tggagactta atggatagta ttgatgaaaa tgcatctaaa atgagtttat
    39241 ctgacatagc aaaacttgaa aaaaagattg caaaaaaaca tattaatcta tggaatgaag
    39301 ggaaaatcga tttttctact actcgtttta gtagtattgt tcctatgtta caaaatgcag
    39361 gagtaaactt tcatttcata taccctgata tagatttatt aaaaaatact tttaataact
    39421 taataaaaga tattcattta catagaatga agggaaatca accaagtgta atatatataa
    39481 cagttaaaga tacaaattat gatgactcta ctctagacca agtttataaa atattaaata
    39541 gtattaaaaa agataaatta accgatttta taattgaaaa aaatagtaat tcttttaaaa
    39601 ttttcactaa atgtgaaacg attaaatcat taactaaaaa ttttactgca tgttatataa
    39661 aagaatctat tgaaaatagt acaaatatta aagtatgtgt tggatatgga attggtaatg
    39721 atataaagca agctatgtct agtgccataa gtgctaataa ggaatctaaa ataaatcatt
    39781 taaattacag tttcttagtt aacgaaaatt atgagacaat aggaccttta ctgtgtgata
    39841 agttacttac tgtttcaaat aaagtaactc cacatataaa tacaattgcc aaaagttcta
    39901 aattgtctac tcttactatt caaaagatta tatctgttac tgaaattcta caaagagatg
    39961 aagttactcc taaggaatta tcttcatgtt taaatgtaac agttagaact gctaatagat
    40021 ttctaagtag tctcttaaag tctggaaacg ctactatatt atatgaaaaa caaaactcaa
    40081 caaaaggtag acctgaaagg gtatacaaat tatttatatc agaaaataaa tattaaaatc
    40141 caaaatgcat aaaaatattt tatcatctaa tattaagtaa aattatatat gactttctaa
    40201 aaggtatatc tttgcatctt tttaagatat accttttatg atatttaata atattttatg
    40261 ttattatcta ctaaaattat tttctaacca ttttactgca caatgagcaa ataaagctga
    40321 accataaact aatccctctt cattaaaaat tgctttagga ttatgcatac caaaattata
    40381 tccctgacta atacttccca ttccaatcca taaaaatact cctggaaccc ttagtaaaat
    40441 ctctgaaaaa tcttctgaac ccattgcaga tggaaagtct attacatttt catttcctat
    40501 aacttcttta acataagata caatctcatc tcctacatcc aaatcattta taactgcagg
    40561 agtccccatt ttaaattcca ctttagcatt tcctccaaat gatttagcta ttcctgtaga
    40621 tatttcaata attctatttt ttaatttttc tctaacaaat tggtcaaatg ttcttaaagt
    40681 tcctgacata gatgctttat ttggtatagc atttggtacg tttcctgcat taaacatacc
    40741 aatagttaaa actacaggtt ctttctgagg tttttctctt gaaagtatag tgtttaatgc
    40801 tatttgtata tgcgacatta tgtttattgg gtccactcca aattcagggc ttgctccatg
    40861 agttgctttt ccttctactg ttatttcaaa tatatccgca gatgccatag ctggaccacg
    40921 tgaataagct acttgacctg tgttaagaaa tttacccacc atactgtgaa atgccatagc
    40981 tgcatcgacc tgaggatttt ctaatatacc atcatcaatc attgattttg cacctctggt
    41041 agtctcttca tctggttcaa atacaaactt tactctacct ttaagtaagt gtttattatc
    41101 cataagtatt ttagctgctc ctaagagcat tgctgtgtga ttatcgtgtc cacaactatg
    41161 agatgcatct ttattcttac ttttaaaagg taaatccgtc tcctccataa taggaagtgc
    41221 atccatatct gcccttaata ataaggtctt tccaccattt tcactgtcta aaacagctac
    41281 tattccatta ttagacgtca atttcggaga atatcccatt ttttctaaag tactggtaac
    41341 aaaggttact gttttcggta tattgcaacc aatttcggga ttttcatgta aatatcttct
    41401 ccatctaata atttcccctt ctattgactg cgcttcttta aaaaaatttt tcatattcct
    41461 tcctcctaat cagatagtca gcaaaatctc actttctttt agtaatgaaa tgatgctgat
    41521 ataaaaatta taattttata tattaaattt catgtctttt taaggagttg gaatatcatc
    41581 tggaatagga catttatatg ttttaccatt catagattta tcaaattctt cttttgcctt
    41641 tttagctatc tctggattat ttaatatttt agctccaaac atagccataa tctttgatgc
    41701 atatatcata cctttttctc ctatagaact tcctgcacaa gatgtaaact gccagctatg
    41761 acctggtgct cctagattcg tacaccctgt aaaaaagtat gctgtcggaa ctatatgcat
    41821 tacatctcca atatcagtag aaccataact attaaagttt gtaacatttc ctccaccata
    41881 atatagatga gttccttctg gcaaattata tttacttatc atagccttat attgttgtgg
    41941 gctttgtcta tctaattcag ctgcaaaatc caattcctct cgagtccaag gctcttgttt
    42001 aatctcattc atacattctg atacaagatt tgctaaaaca tgattgttta aagtattata
    42061 acaacctcct aaaaactcta cttcaacttc agtttcagtc atcattgcag ctccttttgc
    42121 tacctttaca agtctttcat atgtactttc tacagcttct ctacttaaag ctcttgtata
    42181 ataccatact gatgctttat caggaactat gttgggtgct actcctccat ctgtaattgt
    42241 ataatgaatc cttacatcag aagtaacatg ttctctcaaa taattagctc ccacattagt
    42301 aagttccaca gcatctagtg cacttcttcc attttgaggg tctgagcctg catgcgcagt
    42361 ctttccctta aagtgaaact ttacagagtt aacagctgtt gaagtaccaa ttgtagcttc
    42421 actaatttta tttggatgaa aatttatagc taagtctaac ccttcaaacg ctccacctct
    42481 agccataaat cctttacctg ttaattgttc ttctcctgga catgcataaa atactatagt
    42541 cccacttaag ttattttcta tcatctctct tttaagccct actactgccc ctacatgtgc
    42601 tgtacataat aaattatgcc cacatccatg accatatgca cccaattcaa atgcttcttg
    42661 ctttgttgaa actttttgat ttaaaccagg taatgcatca aattctccca taaaacctat
    42721 aactggttta cctgaaccaa aactagctct aatagccgtg gctaaacctc ctacacctac
    42781 ttccacatca aatccttcac ttcttagaaa gtttgctgta ttttcacatg ctttaaattc
    42841 tttaaatgct ttttctggat tttcccatat tttcttactt aaatcatcaa gtttgaatct
    42901 ttttttatct atctcattta tagatatact tcctatcatt tcctattcct cctataagta
    42961 tatttactat caaccaaata taattggacc tattatacta gcaaatgcca ctgatgcaat
    43021 ggttacagta acaaatcctc ctataatcat ctttggtaat aaataatctg ttgccctttg
    43081 tttttcttct tcagttcctt ccatagcatc aataccttcg ttggaaatta tctgtgttgc
    43141 aggataagct agcatacaag ttacaccaat tgcagctgac attcttggag aatatcccaa
    43201 tagttttcct acaattgcag taaataatat tataaataca gagcctacaa caagtattcc
    43261 tatagttgga acaatcatcc caaacaatgc tgttggagtt accttagcaa gtcctccagg
    43321 taacatcaac atacaaccaa acattataat tccattagat tgagatttat ctaaaattcc
    43381 tttttctaaa aaacctattc gtgtaaaaat caaccctact aacaaacatg ctatattagg
    43441 atttaaataa tagtttgcag gggtaaatcc tggtattaaa gtagccaatc ctacaaaatt
    43501 accaatcata gcaacaaatg aaagctttgc aagatacact agagtagatg attttttacc
    43561 taaatcttct ttaaaaattc tcatacttgg aagtttaaaa ttactttttg actcatcttt
    43621 caaaaaatcc ccattatctc ttttaaattt taattcttta tttatacaat atgtagttat
    43681 cggaatgcca aagaactttt gaaatgataa tactagaatt acaaatgcag caagttcagg
    43741 tctatttgca gcttctgcaa cagttgttac gataatacca gcaacagtag aacccgctat
    43801 tggaggtgct gctattaaag catactctct accaaataac atactaccca ctgttaaaca
    43861 gccaacccct attcctacaa tacctactaa tgcaattaca accgttttcc attcatgaca
    43921 taagtcttca agatttataa gtgttcctaa gttagttatt aataaagcta taccaaagtt
    43981 gctcattgca gctaatatcc ctgatgctcc tgtaatgtct tttggtaaaa tatttgtcca
    44041 aaatcctatt aaaaatataa tacatacaaa taacaatgtt gaaattgcac cttttgtttt
    44101 cttagataaa aactcactaa aagcataaat cattacacat acagtaaagc aagtaagtgg
    44161 attccaaaaa ttttccattg ttgtttcctc ctaaatataa taatttttta tcaataaaac
    44221 aataattata aaatgtatat tatgcagtta ttcttaacta catatggtat agcttgatat
    44281 tttttgatga tattatatgc gtcctaaatt attcctaaaa tatctaaata aatataacta
    44341 gagttaactc tctaaatatt aactatagtt atcaacttac tagatttctt tttgattttt
    44401 actttctaca taattttacg agttagaatt gtacttcaac tactctctat attagtatac
    44461 atgttggaag acttatattt atttaaatat tagatataga atacattttt gcaaacgtct
    44521 taaattattc ttaaaatatc taaaacgaaa taataatata cccattaaac caatattatc
    44581 agaatatact tcactttaag tcttctttat atagaaattt tatctattag tgcatcccct
    44641 cctttgtaaa ttagactatt tctaaacgaa tttagatatc ttattcctag gaactattac
    44701 tagaaacaaa aattatttat tatattaaat caatgataag ccatacttta aaattctttc
    44761 tatacaagta tga
//
LOCUS       DAEOSP010000009        43942 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.009, whole genome shotgun sequence.
ACCESSION   DAEOSP010000009 DAEOSP010000000
VERSION     DAEOSP010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.009"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            275..1114
                     /locus_tag="KRH54_000369"
     CDS             275..1114
                     /locus_tag="KRH54_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBG8578048.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            1111..2292
                     /locus_tag="KRH54_000370"
     CDS             1111..2292
                     /locus_tag="KRH54_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578049.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKDDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            2596..3498
                     /gene="ptb"
                     /locus_tag="KRH54_000371"
     CDS             2596..3498
                     /gene="ptb"
                     /locus_tag="KRH54_000371"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBG8578050.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            3520..4599
                     /gene="buk"
                     /locus_tag="KRH54_000372"
     CDS             3520..4599
                     /gene="buk"
                     /locus_tag="KRH54_000372"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG8578051.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            4589..5266
                     /locus_tag="KRH54_000373"
     CDS             4589..5266
                     /locus_tag="KRH54_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8578052.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            5309..5524
                     /locus_tag="KRH54_000374"
     CDS             5309..5524
                     /locus_tag="KRH54_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG8578053.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            5553..6632
                     /locus_tag="KRH54_000375"
     CDS             5553..6632
                     /locus_tag="KRH54_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG8578054.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            6632..7384
                     /locus_tag="KRH54_000376"
     CDS             6632..7384
                     /locus_tag="KRH54_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG8578055.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            7385..7942
                     /locus_tag="KRH54_000377"
     CDS             7385..7942
                     /locus_tag="KRH54_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG8578056.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            8112..9458
                     /locus_tag="KRH54_000378"
     CDS             8112..9458
                     /locus_tag="KRH54_000378"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBG8578057.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            9725..11557
                     /gene="glmS"
                     /locus_tag="KRH54_000379"
     CDS             9725..11557
                     /gene="glmS"
                     /locus_tag="KRH54_000379"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBG8578058.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            12009..13304
                     /locus_tag="KRH54_000380"
     CDS             12009..13304
                     /locus_tag="KRH54_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBG8578059.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVANYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGKESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLIVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            13732..14469
                     /locus_tag="KRH54_000381"
     CDS             13732..14469
                     /locus_tag="KRH54_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBG8578060.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            14490..15743
                     /gene="murA"
                     /locus_tag="KRH54_000382"
     CDS             14490..15743
                     /gene="murA"
                     /locus_tag="KRH54_000382"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBG8578061.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            15830..16894
                     /gene="spoIID"
                     /locus_tag="KRH54_000383"
     CDS             15830..16894
                     /gene="spoIID"
                     /locus_tag="KRH54_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBG8578062.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            16960..17628
                     /locus_tag="KRH54_000384"
     CDS             16960..17628
                     /locus_tag="KRH54_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBG8578063.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            17726..17986
                     /gene="spoIIID"
                     /locus_tag="KRH54_000385"
     CDS             17726..17986
                     /gene="spoIIID"
                     /locus_tag="KRH54_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBG8578064.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            18183..19202
                     /locus_tag="KRH54_000386"
     CDS             18183..19202
                     /locus_tag="KRH54_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBG8578065.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            19218..19646
                     /gene="fabZ"
                     /locus_tag="KRH54_000387"
     CDS             19218..19646
                     /gene="fabZ"
                     /locus_tag="KRH54_000387"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBG8578066.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(19757..20266)
                     /gene="yyaC"
                     /locus_tag="KRH54_000388"
     CDS             complement(19757..20266)
                     /gene="yyaC"
                     /locus_tag="KRH54_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBG8578067.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     regulatory      20455..20558
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            20695..21888
                     /locus_tag="KRH54_000389"
     CDS             20695..21888
                     /locus_tag="KRH54_000389"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBG8578068.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     gene            22204..23301
                     /locus_tag="KRH54_000390"
     CDS             22204..23301
                     /locus_tag="KRH54_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578069.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            23565..25784
                     /locus_tag="KRH54_000391"
     CDS             23565..25784
                     /locus_tag="KRH54_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBG8578070.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            25787..26569
                     /locus_tag="KRH54_000392"
     CDS             25787..26569
                     /locus_tag="KRH54_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBG8578071.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIICHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            26901..28814
                     /locus_tag="KRH54_000393"
     CDS             26901..28814
                     /locus_tag="KRH54_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG8578072.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            28955..29263
                     /locus_tag="KRH54_000394"
     CDS             28955..29263
                     /locus_tag="KRH54_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG8578073.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            29286..29612
                     /locus_tag="KRH54_000395"
     CDS             29286..29612
                     /locus_tag="KRH54_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8578074.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            29651..30985
                     /gene="celB"
                     /locus_tag="KRH54_000396"
     CDS             29651..30985
                     /gene="celB"
                     /locus_tag="KRH54_000396"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBG8578075.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            30996..31517
                     /locus_tag="KRH54_000397"
     CDS             30996..31517
                     /locus_tag="KRH54_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBG8578076.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCYQ"
     gene            31610..32569
                     /locus_tag="KRH54_000398"
     CDS             31610..32569
                     /locus_tag="KRH54_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBG8578077.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIERRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            32618..33670
                     /locus_tag="KRH54_000399"
     CDS             32618..33670
                     /locus_tag="KRH54_000399"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBG8578078.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            33673..34347
                     /locus_tag="KRH54_000400"
     CDS             33673..34347
                     /locus_tag="KRH54_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBG8578079.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDIGVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            34900..35451
                     /gene="raiA"
                     /locus_tag="KRH54_000401"
     CDS             34900..35451
                     /gene="raiA"
                     /locus_tag="KRH54_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG8578080.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            35583..38258
                     /gene="secA"
                     /locus_tag="KRH54_000402"
     CDS             35583..38258
                     /gene="secA"
                     /locus_tag="KRH54_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG8578081.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            38273..39380
                     /gene="prfB"
                     /locus_tag="KRH54_000403"
     CDS             join(38273..38347,38349..39380)
                     /gene="prfB"
                     /locus_tag="KRH54_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBG8578082.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDNDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            39524..41665
                     /locus_tag="KRH54_000404"
     CDS             39524..41665
                     /locus_tag="KRH54_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBG8578083.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            41813..42979
                     /locus_tag="KRH54_000405"
     CDS             41813..42979
                     /locus_tag="KRH54_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8578084.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKALGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      43039..43156
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            43281..43886
                     /locus_tag="KRH54_000406"
     CDS             43281..43886
                     /locus_tag="KRH54_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG8578085.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
BASE COUNT        17793 a         4710 c         8405 g        13034 t
ORIGIN      
        1 agatatttta gaacataaaa ctgaattaag gcgcgagtac tattttgtat tcgcgccttg
       61 atggtttgag ttatttgagt aaaaactaac tttaataaga aatatgtatt tattaagttg
      121 atatttttaa tgtgtttaga gattatttaa caatctccaa atataaaact agaattattt
      181 acataaaagt taaatagttt gtatagagtt agatttatat agtataatac ttagtgtaaa
      241 taattaagaa gttatcaaac aggaggataa aactgtgtta gatataaatt cttttttaaa
      301 tggatttttt aatataatat taagaatgag tatatatgat ttaattgaca tatctatagt
      361 agcgtatatt ttttacaaaa tatttatgtt tataaaagat acaagagcag aacaagtgtt
      421 taaaggtata atatttcttt tattagcaac tcaacttagt aacaccttta aattgcatac
      481 cgtttattgg atatctctta aagctttaga ttatggagtt atagctgctc ttataatttt
      541 tcaaccagaa tttagagcag gacttgaaca tataggaaga gcaaagttta atttatttgg
      601 aaagaatgta aatacttctg aagaaactct caatagaaat atagaagaaa ttgtagaagc
      661 tttatattca ctttctagac aaaaaatagg agctcttatc ataatggaga gagaaactag
      721 aataagtgat ataataaata caggaactat tattgatgct gaaatttcta ggcaactatt
      781 aataaatata tttataccaa atacaccatt acacgatgga gctgttgtaa ttagagattc
      841 taaggtaaaa gcagctgcat gttttttgcc attgacagaa agtaaggatt taagtaaaga
      901 tttgggcact agacatagag caggaatagg ggtaagtgag gtgtctgact gtataactct
      961 gattgtatca gaggaaacag gtggcgtatc aattgctaaa gcaggtaagt tatatagaga
     1021 tatttctaga gaaagaatga tgaatatatt acgtagtaat ttaaaaacaa atacagaaac
     1081 aagaagtttt ttcaaaggtg gtatatttaa atgataaata gactaaaaaa taataccaaa
     1141 ataaaaataa tatcattttt aagtgcaatt gcattatgga tgtatgttat ggctgtagtt
     1201 gaccctgaag aaactaaagg gtttgaagat atacctgtat ctataagtaa tatgagtgat
     1261 ttaaaggata aaaatcttgt tatatatcca gacgaaaaat taacagcaga tgtatatata
     1321 aaaggtagtt tatcaaaggt acaaagtgtt aaaaaagaag atatacatgt atatggaacc
     1381 attgaagacc caatacaagg acagaaggaa gtatatttaa aagcaaatat tcctcaaggt
     1441 gttactctag aatttaagca tgatgtttta gtagtaaatc ttgaaaaaaa cataaaagaa
     1501 aaaagagata taaaggtaga agtaagtgga aattcaaaaa gaaatataaa taaaattgaa
     1561 cttagtaagg aatttgtcga tgtatcaggt ccaagaactc tagttaaaga agtcaaaagt
     1621 gtaagagcta atctagatgt tggtaatgag acagatgatt tttctaaaaa attgcaatta
     1681 ataccattaa atagtgatgg aactaaggtt gaaggagtaa cattaagttc atcctttgtg
     1741 actgcaaaga tagagctatt gaaggaaaaa attgtcccaa taagagtaag tctaaatgat
     1801 aataaagata atgatgtact gaaaaactat aaattaagta aggaacaagt tacaataaaa
     1861 ggaaagaaag atgatataga caaaataagt tatattaaga caaaacctat tgacttagct
     1921 gatttggcta atggtgaaga aaaagaagtt agcttagaaa ttccagaggg tataactctt
     1981 gaggatagct ctataacaat taaattaaat gatacaaaac aattgagtgc agaattttta
     2041 tataatgcgg gagatattga attaagaaat atcccaacaa atatggataa tccaaatgta
     2101 atttctgatg tagatataaa agttgttgta gagtcaacgg aggatttgag taagttaaat
     2161 aaaaaagata tttcattata tattgattta tcacaaggtc ctgaggaaga tagtaaatat
     2221 aaaataaaat atgaaacaac atatcaattt aaaaaaataa atatagaacc taatattgtt
     2281 gaagtggaat gaacaaatat ttcgaatatt tagttgttac aagatgcgac aaaaaaacct
     2341 ataaaattaa gtttgttaat tttatgtttt tgttgcatct tgtttatttt agttatatta
     2401 taaaataagg acaatgagat atttgttaaa tctaaaaagc gctagatata tgaaaaaaga
     2461 ctgataaata gatttaaata aatattttga attaaatatt tcaattatct ctaggaaaac
     2521 gttgctgaac gagttataat ttaattgaga tataaaaagt tctatttcaa aaaattcaag
     2581 atgaggtgga aatgcatgag aagttttgaa gaagtaatta agtttgcaaa agaaagagga
     2641 cctaaaacta tatcagtagc atgttgccaa gataaagaag ttttaatggc agttgaaatg
     2701 gctagaaaag aaaaaatagc aaatgccatt ttagtaggag atatagaaaa gactaaagaa
     2761 attgcaaaaa gcatagacat ggatatcgaa aattatgaac tgatagatat aaaagattta
     2821 gcagaagcat ctctaaaatc tgttgaatta gtttcacaag gaaaagccga catggtaatg
     2881 aaaggcttag tagacacatc aataatacta aaagcagttt taaataaaga agtaggtctt
     2941 agaactggaa atgtattaag tcacgtagca gtatttgatg tagagggata tgatagatta
     3001 tttttcgtaa ctgacgcagc tatgaactta gctcctgata caaatactaa aaagcaaatc
     3061 atagaaaatg cttgcacagt agcacattca ttagatataa gtgaaccaaa agttgctgca
     3121 atatgcgcaa aagaaaaagt aaatccaaaa atgaaagata cagttgaagc taaagaacta
     3181 gaagaaatgt atgaaagagg agaaatcaaa ggttgtatgg ttggtggacc ttttgcaatt
     3241 gataatgcag tatctttaga agcagctaaa cataaaggta taaatcatcc tgtagcagga
     3301 agagctgata tattattagc cccagatatt gaaggtggta acatattata taaagctttg
     3361 gtattcttct caaaatcaaa aaatgcagga gttatagttg gggctaaagc accaataata
     3421 ttaacttcta gagcagacag tgaagaaact aaactaaact caatagcttt aggtgtttta
     3481 atggcagcaa aggcataatt ttaaggggga aataggcaaa tgagcaaaat atttaaaatc
     3541 ttaacaataa atcctggttc gacatcaact aaaatagctg tatttgataa tgaggattta
     3601 gtatttgaaa aaactttaag acattcttca gaagaaatag gaaaatatga gaaggtgtct
     3661 gaccaatttg aatttcgtaa acaagtaata gaagaagctc taaaagaagg tggagtaaaa
     3721 acatctgaat tagatgctgt agtaggtaga ggaggacttc ttaaacctat aaaaggtggt
     3781 acttattcag taagtgctgc tatgattgaa gatttaaaag tgggagtttt aggagaacac
     3841 gcttcaaacc taggtggaat aatagcaaaa caaataggtg aagaagtaaa tgttccttca
     3901 tacatagtag accctgttgt tgtagatgaa ttagaagatg ttgctagaat ttctggtatg
     3961 cctgaaataa gtagagcaag tgtagtacat gctttaaatc aaaaggcaat agcaagaaga
     4021 tatgctagag aaataaacaa gaaatatgaa gatataaatc ttatagttgc acacatgggt
     4081 ggaggagttt ctgttggagc tcataaaaat ggtaaaatag tagatgttgc aaacgcatta
     4141 gatggagaag gacctttctc tccagaaaga agtggtggat taccagtagg tgcattagta
     4201 aaaatgtgct ttagtggaaa atatactcaa gatgaaatta aaaagaaaat aaaaggtaat
     4261 ggcggattag ttgcatactt aaacactaat gatgctagag aagttgaaga aagaattgaa
     4321 gctggtgatg aaaaagctaa attagtatat gaagctatgg catatcaaat ctctaaagaa
     4381 ataggagcta gtgctgcagt tcttaaggga gatgtaaaag caatattatt aactggtgga
     4441 atcgcatatt caaaaatgtt tacagaaatg attgcagata gagttaaatt tatagcagat
     4501 gtaaaagttt atccaggtga agatgaaatg attgcattag ctcaaggtgg acttagagtt
     4561 ttaactggtg aagaagaggc tcaagtttat gataactaat gataaaagaa taagaatcat
     4621 aactggtcac tatggaagtg gtaaaagtga atttgcgatg aactatgttg ttaagttaag
     4681 agatatggtt tctggcaaag ttgctatagc cgatttagat gtggtaaatg tatactttag
     4741 aactagagaa aagaaagagt taatgaaatc cttaggtata caaccaatag atagctcaat
     4801 taatgctcca acattagatt taccagcagt atcagcagag gtaatgtctc ctatggtaga
     4861 ccattcatac aattctgtta tagatttagg tggagataat gtaggtgcta gggttatagg
     4921 aagatttagt catttgttaa aagaaggcga ttatgatatg ctttttgtaa taaatgcaaa
     4981 tagggagaaa actcaaactt ctgaagaagt tatccaatat ataaaagaaa tagaaaaatc
     5041 ttctaaatta aaggtaacag gtctaattaa taatactcat cttattaggt ttactactat
     5101 tgatgatgtt ttaagaggtc agaaagttgc aaaagaagtt tctgaaaaat gtaatatacc
     5161 gataagatat gttgcatgtt tagaaaatct tgttgaacag ttaccaaaag atttagaagg
     5221 agaaattttt cctataaaat tatatatgag agaagactgg atgtagtttg agctcaaata
     5281 tatagaaagt ttaaggaggc ttaggtaaat ggctaaagga aaagtatctt ttaatcaaga
     5341 acgttgtaaa ggttgtggat tgtgtgttga agcatgtcca gtaaaaatca tacaattaga
     5401 ctcaaatgta ataaacaaaa aagggtataa cccagcaact gtttttgaaa tggaaaaatg
     5461 cataggatgt gcaagttgtg caactatgtg tccagatgta gttataacag tagaaagaga
     5521 ttaatttaat taatatttaa gggggaattt taatggctaa gatacttatg aaaggtaacg
     5581 aagcatttgg aaaagcagct atagaagctg gttgcaaata tttcttcggt tacccaataa
     5641 ctccacaaag tgaattacca gaatatttat caagagagtt acctaaaata ggtggagcgt
     5701 tcgttcaagc tgaatcagaa gtttctgcga taaacatggt ttatggtgga gcaggtgcag
     5761 gagcaagagt tatgacttct tcatcttcac caggagttgc tttaaagcaa gaaggtataa
     5821 catatgcagt aggagcagaa gttccttgtg ttgtacttaa tgtaatgaga ggtggtccag
     5881 gacttggaag tatacaacct tcacaagctg actactttat gtctacaaga ggtggaggaa
     5941 atggagacta cagaactcca gtattcgcac cagctacagt tcaagaagct gttgatatga
     6001 taatggaagc ttttgacgtt gcagattatt acagatcacc agttatggta gttgcagatg
     6061 gtatgatagg tcaaatgatg gagccagttg aatttagagc tcctgagaag aaaagagaat
     6121 tgcctcctaa agattgggca actgttggaa ctaaaggaaa aagaaaacct aatgtaataa
     6181 actctctata cttagagcca gaagtactag aagaccattg ttggcattta caagaaaaat
     6241 ttgatgcaat ggaaaagaat gaagtacaat acgaaatgta caaaacagaa gatgctgaat
     6301 ttgtatttgc agcttatgga actacttcaa gagtagtaaa aagcgcaatt gatatattaa
     6361 gagaagaagg aataaaagca ggtcttataa gacctaaagt attatggcca ttcccattcg
     6421 aagcattcaa ccaaattcct aatgctagaa acatattgac tgttgaaatg agtatgggac
     6481 aaatggtgga agatgttaaa atggcagtag aaggtaaatt accagtttac ttccatggaa
     6541 gaccaggagg aatgactcca acaccagcag aaatagttga aaaagctaaa aaaattattg
     6601 ccggagaatt ggttgcagga ggtgctagat aatggctgtt gtatttaaga aaactgaagg
     6661 attacaagat actcaaactc actactgtcc aggatgtaca catggtataa tccatagatt
     6721 agttggtgaa gtattagaag aactaggagt attaggtgat gctgtaggtg ttgttccagt
     6781 tggatgttca gttttaggat ataaatattt taactgtgac actcaagaag ctgctcatgg
     6841 tagagcgcca gctgctgcta caggaataaa aagagttcat cctgaaaata cagtattcac
     6901 ttatcaaggt gatggagact tagcttcaat tggtactgct gaaatagttc atgcagctgc
     6961 tagaggagaa aaaataacta ctatatttgt aaacaataca acttatggta tgactggtgg
     7021 acaaatggct ccaactacat tagttggaca aagagctact actgctcaat caggaagaaa
     7081 tgctgaaact caaggttatc caataagagt aagtgaaatg ttagctacat taacaggagc
     7141 tgtatttgta gaaagagtag cagttgatac tccagctcat gttagacaag ctaaaaaagc
     7201 tataaagaaa gcattccaag ttcaacaagc aggattagga tttggaatag tagaagtatt
     7261 atctacttgt cctacaaact ggggactagc tccaaatgat gctttacaat ggttaagaga
     7321 caacatgata ccatactatc cattaggaaa tttcaaaaat gttgaagttg aggaggtgaa
     7381 gtaaatgtct acagctagag taatatgtgc aggcttcgga ggccaaggtg ttatgtctat
     7441 gggacaatta cttacttatg caggaatgct agaaggaaaa gaagtttctt ggttaccatc
     7501 ttatggacca gaaatgcgtg gaggtacagc aaactgtgca gtaactgtat ctaatgagcc
     7561 agttggttct ccacttataa cagatgatgc aactgcagct atattattaa atataccagc
     7621 atttgaaaaa ttcaaagatg atgttattcc tggtggaaaa ataatagtaa atagttcttt
     7681 aataaaagaa aaagttgata gaacagatgt agatgtttat tatataccag caaatgaatt
     7741 agcagctgaa ttaggaaatg ataaagttgc taacatgata atgttaggcg cttacttaaa
     7801 agtatctgat actgtagata tagaatctgt actagaagca ttcaaaaaag ttttcggacc
     7861 tagaaaagaa aaattcgttc cattaaacag agaagcttta caaaaaggta tggatgctac
     7921 atgttctaaa gcaactaact aataaagaaa aagatgtcaa aaaaaagaca atagaattat
     7981 ttctattgtc ttttttgctg ataagtatta aaatattttt atgactgaaa ttaagcctat
     8041 aatgatattt tatgaactat aaaaatataa tacaaattac aagcttttca attatgaaag
     8101 gagaatcttt agtgagaaag tattttggaa cagatggagt tagaggagta gcaaatacag
     8161 aactaacatg tgatttagca tataaacttg gtagagctgg tggatttgtc ttagctcaag
     8221 gagaccacag agtaaaagta gtagtaggta aggatactag aatatcaggt gatatgttgg
     8281 aagcatcttt aatagcagga cttatgtctg ttggatgtga tgttattact gttggaataa
     8341 taccaacacc tgcagtggca tatttaacta gaaaatatgg tgctgattgt ggtgtggtta
     8401 tatctgcatc tcacaatcct gttgaatata atggaatcaa attctttaat aagaatggat
     8461 ataaattaga tgatgaaatt gaattgaaaa tagaagaata tatagacgat atagacaaaa
     8521 ttgattgtct tccaattggt gaaaatgtag gaagaaaatt acatgaacat tgtgcacaaa
     8581 gagactatgt agactaccta aaatcaataa taagcactga ctttaaggga ttaaaagtag
     8641 ttttggactg tgctaatggt gcgtcatata aagtggcacc aatagtgttt gatgaactag
     8701 gagcaagtgt aatttcaata aatagttcac cagatggaaa taatataaac tataaatgtg
     8761 gctctacaca tccagaacaa cttcaaaggg ctgttttgga acataatgct gacttaggtc
     8821 ttgcatatga tggagatgct gatagattaa ttgcagtaaa tgaaaagggt caaattgttg
     8881 atggagacca tataatgata ttgagtgcct taaatttaaa gaaaaataat aaattggcac
     8941 aagatacttt ggtagttaca gttatgagta atatagggtt gacaatagct gctaaggaaa
     9001 atggaataaa tttatctact acagcagttg gagacagata tgttttagaa gatatggtta
     9061 aaaatggata taatcttggt ggagagcaat ctggacatat gatattttta gattacaaca
     9121 ctactggaga tggagtgctt agttctctaa tacttgcaaa tataatcttg caagaaaaga
     9181 aacctttatc agaaattgca tcaataatga gccaataccc acaagtatta gtaaatgcaa
     9241 caataaaaaa tgaaaataag aacaaatata tggaatatcc agaaataaaa actgagatag
     9301 aaagaataga atctatttta gacggaaatg gtagagtttt gataagacca tcaggaactg
     9361 agccattagt tagagttatg ttagagggta aagaggaagg tcaaataaaa gaattagcaa
     9421 caaacttagc taatcttata caagaaaaat tatcgtaatt taaagggtat tctcagatat
     9481 aaatactgag gacacctttc taagcgccag aacttagtta tagacataat tattgagtaa
     9541 tataggaact aagttgacga ggtcagagtt aatcgaattg tcggcggatg ctctgcggtg
     9601 tgtttaccat cgaaagactt ctttaaataa taggagcaat cctatggaca aagggaagcc
     9661 aaagtaaact tatatgacct aactcttaaa atatataaat aatattttta ggaggacaca
     9721 aataatgtgt ggaatagttg gatatttagg aagtagaaag gcagcagaag ttatagtaga
     9781 agggctttct aagctagagt acagaggtta tgactcagca ggagtagcag ttaatagttc
     9841 taatgaaaaa gaacttaata ttagaaagtt taaaggtaga ctttctgtac ttgcagaaga
     9901 cttagaaaag aatcctattg atggaaattt aggtataggg catacgagat gggcaactca
     9961 tggagaacca tctgatgtaa actcacatcc tcattttaat caagccaaaa caatagcagt
    10021 tgttcataat gggataatag aaaactatat ggaaataaaa gaagaattaa tttctgaagg
    10081 tgttaagttt gaatctcaaa cagatacaga ggtaatagca catttagtag ataaatacta
    10141 tgagggtaat ttattagatg ctgtatacaa aactatatca aaattaagag gagcttatgc
    10201 attaggtgtt atatgtaaag aacatggaaa tgaattagtt gcagtaagaa aagatagtcc
    10261 tcttgtagtt ggtgttggtg aaggtgaaaa ctttatagca tcagatatac ctgcattatt
    10321 aaaatatact agagatgttt atttcttaga aaatggagaa gtagttcatc taaaagacga
    10381 aaatgtaaca gtttatgact ctaatagaaa tcttgttgaa aaagaagtat ttcatgtaac
    10441 ttgggatgta gaagctgcgt ctaaaggtgg atatgactac tttatgtcaa aagaaattca
    10501 tgagcaacca acaggagtaa gagaaacttt agaaagaaga cttgatgata atggaaatat
    10561 aattctagac agtataaata tttctaaaga agacttagaa aaaataaata aagtttatat
    10621 agtggcttgt ggaacagcat ataatgctgg attacttggt aagtatgcca tagaaaaatt
    10681 tgtaaatata ccagtaataa ctgatatagc atcagaattt agatatagcg acccatttgt
    10741 agatgaaaat tcattagtaa tattagttag tcaatctggt gagacagcag atactctagc
    10801 agtattaaga gattcaaagg ctaaaggagc aagaatactt tcaataacta acgttgtagg
    10861 ttcatctata gcaagagaat cagatgatgt attctatact tgggcaggac ctgaagtagc
    10921 agttgcatca actaaggcat atactactca aataacttct ttatacatga tagctttaga
    10981 ctttgctatc aaaaaaggaa caataactag agaattttat gatagtatga tttctaagat
    11041 gaaagaaata ccatcaaaaa tacaagaaat actagacaat gaagaatata taaaagaagt
    11101 tgctaaaaca gttgttagtt ctgaacatgc attctattta ggaagaggta tagactatag
    11161 cttagcaatg gaaggttcat tgaaattaaa agagatttca tatatacatg cagaagcttt
    11221 tgcagcagga gagctaaagc atggaacaat agctttaata gaaaaaggaa ctcctgtaat
    11281 tgccatagct actcaagaaa agttatttga aaaaatggtt tctaatatgg aagaagtaag
    11341 agcaagagga gcttatgtag tagcaatagc tcaaagccat aataaggatg ttgagaaagc
    11401 tgctgataaa ataatctata taccaaattc agatgatatt ttatcaccaa tattagcagt
    11461 tgtaccaatg caattacttg cttatcatgt ctctgtacta agaggatgcg atgtagataa
    11521 gccaagaaat ctagctaaat cagtaacagt tgaataaaaa cagattaaaa attaaataga
    11581 taatatttat tgaaaagaac tcatcctgtt atttgtattt tataggatga gttttttaga
    11641 taaatattaa atttgttttg tttttgctat aataaatttt ataaaaagat tgatattaaa
    11701 tcatctgtaa aatgataaat atatagacca taccaggtat agggaacgat aaatatagtt
    11761 catataaaat atagtgagta cttaataaac atatagtcaa tatcagataa aaaaaataat
    11821 agcttatgag aaaaattaaa atacaaggta ttagtaacct tgtgataaga taaagtgagt
    11881 agtttaaaat aagaatataa ggaactattt ataaaagaat ataatgaagc taatagagta
    11941 taagaaaatt actttatttt agtaaaataa tttcaaataa aaattttttt aaattgggag
    12001 tataaattat gacaaatgat tttaaaaaag aaaagaaaga agattatttt gttaatctta
    12061 aggcaatatc tactgaagtt ttacaagaaa aagtgcaaga taacgcttgg gttatagttg
    12121 atactcgctt aaatgatgct tataatggct ggaagttaga tggagtaaaa agaggtggac
    12181 atataaaagg agcagtagat ttttctgcta attggttaag tgtatactca gatagaaaag
    12241 atgaagtgtt agaacaagca ttgaaaacta aaagaattga tttagataaa aatatagtat
    12301 tgtatgatgc aaatggaaaa gatgctttgg ttgtagcaaa ttatttatct aaaaaaggat
    12361 ataaatatct atataaatat gatattaaac aatgggcaga cgacgaaaat cttccaatgg
    12421 aaagatataa aaattatcaa atgatagttc ctgcttttat aataaaagat atattagatg
    12481 gaaaaattcc tgagacattt gaagattcaa agaatataaa aatgattgaa gcgagttggg
    12541 gaaaagaatc gtatacaaaa gggcatatac ctacaagtgt tcatgtaaat actgatatta
    12601 tagaacctcc tccaacatgg atgcttgata atgatgataa cttaactaag tttgctttag
    12661 actatggatt aacaaaggat gatactgtaa tagtttcaag tagtacacct atggcatctt
    12721 atagactagc tgttatattg agatatatag gagtaaaaga tgtaagagtt ttgaatggtg
    12781 gaacaaactc atggttatca gcaggatatg aattagaatt tattagtaat ccaaaacatt
    12841 catgtactaa ttttggagca gatataccag ttaactcaca attaattgtt actacatctg
    12901 aattaagaca aaaactaaaa gaaaaaaata agtttatact agtagataac cgtacttggg
    12961 atgaacatat aggaaaagta tctggatata catattatga taaaaaaggg cgtataccag
    13021 gagctttata tggacattca ggtagtgata gtgtttcact agaagaatat cgtaatattg
    13081 ataatacaat gagaaataaa tatgagattt tagaaatgtg ggacaaggaa aatatagatg
    13141 tgaataaaca gcttatattt atgtgtggaa gtggatggcg tgctgctgaa gtattaacat
    13201 atgcaaatgt cataggagtg gaaaatacct ccctttatag tgatggatgg atgggctgga
    13261 gtcttgataa ttcaaattta attgaagtgg gagaacacaa ataaagtttt tgaaagtgca
    13321 atagagttgt ttaaaacaaa ggtgtttttg atttattagg aagagtgtgt aaattgtgta
    13381 ttgggaagtg tacttaggta tgttcatttg gagctatgga aattgacaaa aaagtattat
    13441 ctgtttattt tgttggtaaa atatgaatag attatagatt taagtataga ctaagtaatt
    13501 tatattacag aagagaatat ataagaatta atgcaagatg tatttgatac atataagaaa
    13561 ttggcaagtc caggagaagg atagagaaaa ttgagattga agagtttgaa agagattatt
    13621 agatgaaaaa tacaaaaatg tataaaaata catattgaga aagtatatct ctcttaaatt
    13681 tgttaataat tctattatac agaaaaacat atttaagggg gattttttac tatgaaaaaa
    13741 gtaaaattag ctataagctt ttttatatta atgattatag gaatgacaat atcttatgca
    13801 gatacaagta taaatactag atacaataga ttaagtaaag ctcttgaaat atcagaagca
    13861 aattttaaat tttataatgt taaaattaat tctgttataa atcataattt atcaaataaa
    13921 gaatttcata atatttttat agaaataatt aacaatctaa agttggataa aaaaggaata
    13981 aattggaatc aaaaagggtc tggcgataaa atacaattat gtgctactat aaaaggtagc
    14041 gataaagaaa tttctataaa aggaataaat aaaaataaca aagaaagtta catagtagtt
    14101 gacatattgg ataacaaagt atataaacat aaagaggtta tttatagtaa cgtagaaaat
    14161 gtgttaaata aatattcatc tcaagtagat gtgtctgctt gtattgttgg agaatacaca
    14221 aaaaggttac aaaacaacaa atatgatgat attttacaaa aaatattata taatatgagt
    14281 gctaagcaaa tagatgaagt taaagacgta aactttttat ctataacagc atatagtaaa
    14341 gtattaaatg attatgaatt agaatattta ggggaaaaaa ttaatttaaa tataggaatt
    14401 aaatataatg aggatgacgg aaaaacactc atatatattg ccacaccaat tataaaatta
    14461 gattattaat tatgaggaga tcaaaaaata tggctaagat aatagtaaaa aagagtaatc
    14521 cacttaaagg tagtgttaaa atagatggtg caaaaaatgc agttttacca ataatagcag
    14581 cgactttatt agcaaacgga aaatcaacat taaatggagt accaaattta agagatgttc
    14641 atgttatatc agatttatta agacatgtag gagcagaagt tgaatataaa gaaaatacac
    14701 ttactgttga tgcaagcaat ataaaaactt gtgaagctcc atatgagctt gtaagaaaga
    14761 tgagagcttc ttttttggtt atgggaccat tgcttgctag atttaatagt acgaaaattt
    14821 ctatgccagg aggatgtgct ataggaacaa gacctataga tttacattta aaaggattta
    14881 aagccttagg tgcaaagata gaaatggacc atggttttgt tgaagcagca acagaaaagt
    14941 tagtaggaaa taaattatat ttagatttcc catctgttgg agcaactgaa aacataatga
    15001 tggcagcttc tttagcagaa gggactacaa taatagagaa tgcagcagaa gagccagaaa
    15061 tagtagattt agcaaacttc ttgaacgaaa tgggagcaga tgttaaaggt gcaggtacta
    15121 atactataaa aataaaaggt gttaaagagt taaaaggagc agaacataat gtaattccag
    15181 acagaataga agcagcaact tatatggttg cagcagctat gacaaaaggt gatataactg
    15241 ttgaaaatgt tttaatggag catttaaaac cagttgtagc taaattaaga gaagctggtt
    15301 gtgaaataac agaaatggac aattctgtaa gagttgtagg tccaaaagta ttaaaaccaa
    15361 tagatataaa aactttacca catccaggat ttcctacaga tgttcaagca caatttatgg
    15421 ctatgcttac tgtagccaat ggaactggtg tagttataga aactgttttt gaaaatagat
    15481 ttatgcatgt tgctgaattt aatagaatgg gtgctaatat aaaaatagat ggaagaagtg
    15541 cggttgttaa tggtgtagat gagctacacg gtgcagcagt aaacgctact gatttaagag
    15601 caggggcagc ccttatttta tgcggtctta tagcagaagg agaaactcaa ataggagaaa
    15661 tataccatat ccaaagaggt tatgttgata tagataagaa gataacagca ctaggtggtc
    15721 aaatcgaaat agtagaagat taataattgg tttatagaag aaagttataa attaataggg
    15781 ttgttcatat acttaaatac aaggttttaa aagctaagga ggagagtata tgaagaaccc
    15841 attggttgtt ttgttaggat ttgtgacttg ctctgtgtta gttccatcac ttataacttt
    15901 agtttcttat aaaaatgtag aattaactga aaaaccagag agcccagtat ctataaacaa
    15961 aactattaaa aaaagtgata ttgaagacaa aggtaataaa gaagaaaaaa atacagtgaa
    16021 ctatgaaact gtaaataaaa aagcaccaat tatcaatgta tataatcata taacagggaa
    16081 aaccgaaaag atggacatgg agaactattt atgtggagta ctagctggtg agatgtcttc
    16141 agagtttgat atagaagctt taaaagctca atctgtagca gctagaacat atgtagtata
    16201 taaacaggaa catggtaaat ctagtaaaca taaaaatgca gtagtatgta ctgattataa
    16261 acattgtcaa gaatataaga gttatgatac tcttaaaaaa ttaaatggtg aagaatggat
    16321 taagaataaa tattccaaaa tccaagaagc agttagagga actaaaggtc aaataataac
    16381 ttataatgat aaggcgatac ttcctcttta tttttctaca tcttcaggaa aaacagaaaa
    16441 tagtgaagaa gtattttctg caaaatatcc ttatttaaag tctgtggaaa gtccttatga
    16501 taagtattcc cctaaatttg cgtcaacact taaaataagt aacacagatt ttgttaaaag
    16561 tcttagaagg gcatatagca ctattgtgat agatgtgaat aatttaagta aacaagtttc
    16621 aataactaaa agaagtgatg ccggaactgt agaaaaaata aaattaggaa ataaagaatt
    16681 gactggaaaa gacattagaa ctgtttttaa attgaactct gcaaactttg acataaaatt
    16741 tggagaagga tacatagatt tcgtggtaaa aggatatggt catggtgttg gtatgagcca
    16801 gtggggagca gaaggaatgg cagaagaggg ctataaatat tatgatatat tatcacatta
    16861 ctacacagat acaaaaataa aagatatata ctaataattg tataaaatgc caaaaacacg
    16921 tcaataatcc aaatgtagaa atatatttgg aggtgcaaaa tgaagaaaaa gctgttagaa
    16981 aaagatggtt tttatttatc tttgtttgta tgtgtttgtt tattagcagt aggtggagtt
    17041 tggtttacaa ataataatgt agataaattg gcttctaata aaggaattat ggaaaatgct
    17101 aataaagata gtgaagagga aatacattta attgaaaaag acaagaaaga tgctatccct
    17161 actgcaacgg attcaaaaca aaacttagaa aaagctaagt caaaagagga aaataaatcg
    17221 agtacaacaa aattaaatta cattggagat aaagtaataa gaggatattc agaaaaagag
    17281 cctagttatt ctaaaacact tgatgtttgg gaaactcata aaggtgtaga tattagttgt
    17341 actaaaggca aagaagtaaa atctctatta aacggaatag tagtagatgt atttgatgat
    17401 gaagaatatg gacaatcagt caaaataaaa agtgacaata acattgtagt agtatattca
    17461 aatctagata aaaatgttag tgttaagaaa gaacagaagg taacagaagg acaatctcta
    17521 ggtactgtag gtagtacttc tcaaatagaa agtgaagaag gtatacatgt gcatttagag
    17581 gcgtatagtg gagaaaaatc tatagaccca atgagtctaa ttaagtaata gtataacaag
    17641 tttaatcata tttggttaag cttgttatat tttttggtgt ttttacatat atatcattaa
    17701 agctattatg tgaggggggt aatctttgag atctcatata gaggaaaggg ccatagtggt
    17761 agcaaaatat atacttgaaa aaaacactac agtaagacaa accgccaaga catttggagt
    17821 aagtaaaagt acaattcata aggatgttac agaaagattg aaggaaataa atccatccct
    17881 tgccaaagaa gttaaaaatg tgctggacaa aaataaatca gagagacata ttagaggggg
    17941 attagcaacg aaattaaaat atgagaagga acataaaaaa atgtaggtta aaaacctact
    18001 ttttttattg aattaaatta taaaaagaaa tatattaagt aattataaat agcaaaataa
    18061 taatactctc aaaagattta ttttctcttg attaaagcga gatttttgta tatgctttat
    18121 tataggagta ttaaaatagg cttataatat aatctaatat tataaataaa ggagttgaaa
    18181 aaatggctgg agctgatata ggcatagatt taggtactgc aaatgtgctt gtttatgtta
    18241 gtggtaaggg aatcgtttta gaagaaccat ctgttgtggc gatagacaaa agaacagatt
    18301 ctgttttagc tgtgggagaa gaagctaaaa agatgatagg gagaacacca ggtaatatag
    18361 tcgctataag acctttaaga gatggtgtaa tttcagatta tgatgtaact gagaagatgt
    18421 taaaatcttt tatagataag atagttgata aaaagggatt tggaagattt tttatgccaa
    18481 gaattatggt atgtgtacca acaggtgtca cagaagtgga aaaaagagca gtagaagatg
    18541 ctacgagaca agctggagct agagaagtat atatcataga agaaccaata gcagctgcca
    18601 taggagctgg tgtagacata tcacagccta atggtaatat gattatagat attggtggag
    18661 gtactgtaga tatagctgta atatctctag gaggagctgt tgtaagtgaa tctataaagg
    18721 ttggtggaga tagatttgat gatgctatag ttaaatatat gaaaaaacaa cacaatcttc
    18781 ttataggaga aagaacagca gagaaaatca agtttgaaat aggttctgca tttaagagag
    18841 aagaagaaaa atatatgaag ataacaggaa gaaatttaat tacaggactt cctagttcta
    18901 taactataaa ttcaacagaa atgttggaag cacttagaga atgtgttgag caaatagtag
    18961 ttgctactca tgctgttttg gaaaaaacgc ctcctgagtt agcagcagat ataggagatg
    19021 tcggtataat aatgactgga ggaggttcat tattatatgg attagataaa ataatagaac
    19081 agaggacagg aataactgtt acaatagcag atgaaccatt atcttgtgtt gcaaaaggta
    19141 caggaatagc tttaggttca atagacttac ttgaaactgg tgggtctttc aaaagaaaat
    19201 aattaggaga gtttagaatg ttaaatatag atgaaataaa aaaattaata ccacatagat
    19261 atccattttt attagtagac aagattacag agctagaagt tgggaaaaga gctgttggta
    19321 taaagaatgt aacagttaat gagccatttt tccaaggtca ctttccagaa tatcctttga
    19381 tgccaggagt actaatagta gaagctctag cacaagtatg tggagtggca atgatgtctg
    19441 tagaggaaaa caaaggaaaa ctaggtgtat ttgcaggtat agataaagtt agaatcaaga
    19501 gagaagtaag accaggagat acattaacta tggaagttga aatgacaact cttagaaaaa
    19561 atatagctaa ggcagatgct aaggcatatg taggtgaaga acttgtctgt aaaggtgaat
    19621 taatgtttgc tttagtagaa aaataaaaac ttgaaatata aaagtaaaaa ggtagataat
    19681 ttggagtatt taatcagata ctctaaaata tctacctttt tatataaaga attttatcat
    19741 ttagtttata tttagattag gtggatacta ataaagccaa ggctattgtt tcagctaaat
    19801 ctaatataaa gctaaggcgt atattattaa atgaaagttt attattttca tcaattttat
    19861 ctacaattcc tactatagaa taatttccta tttgaggtag ttttttacca acaccttttc
    19921 cgggcaggat aggtccttct ctaatctgta tattacctat gttactctgc gaccccaagc
    19981 aagcatctat agccaagaag ttaccttgta tatgtgtatt ttttatagta tctaaagact
    20041 catatatatt taaggcgtga atagggttat ctagtgtacc gtaaacgggg tatttaaagt
    20101 tactgttctt aagtattgtt ccaactaaag ggcctaaagt atcacctata gccctgtctg
    20161 ttcctatgca aacaacaatc gtattttcat taattatagt ttttagaact gaacttagca
    20221 tctgtataac atcatcatcg ctgtagttta ctttagctaa atacaatagg aacaccccct
    20281 tcaaaaaaat atatgaataa aaagcattaa aaagacctaa ctttaaattt cagatagata
    20341 gaaaatttta tttgtcttac aaaaaaagga atagaaaaaa tatgactaaa aatattgaca
    20401 aaattttaaa aaaatagtaa gctagtaaag taaaacaaaa cttaaatctt gattacctta
    20461 tcaagagagg ctgagggata ggccctgtga agcccagcaa ccacccaaat gggaaaaggt
    20521 gctaattcct acaagacaaa agtcttgaaa gatgaggtca gttaattagt atgtaattac
    20581 gattattaac ctcttcttat aaaggagagg tttttttatt ttgagtaaat ttctaaaatt
    20641 aaaaataagg taaaacctat aataaaaatt aagaataaat aagggggaat aaaaatggca
    20701 agacatttat ttacgtcaga atcagttaca gaaggacatc ctgataagat atgtgaccaa
    20761 atatcagatt caatattaga tgcattatta gaaaaagacc cacaatctag agtagcttgt
    20821 gaaactacag ttactactgg tttagtttta gtagcaggag aaataagtac atctgcttat
    20881 gtagacattc ctaaattagt aagagaaaca gtaagagaaa ttggatatac aagagcaaaa
    20941 tatggatttg attgtgatac ttgcgcagtt ataacttcta ttgatgaaca atctggagat
    21001 atagctatgg gagttgatga aggcctagaa agtaagactg gagaagagat agaagaagaa
    21061 atagaaaaag ttggagctgg agaccaaggt ataatgtttg gatttgcatg taatgagact
    21121 ccagaattaa tgccacttcc gatatctttg gcacataaat tatcaagaag acttacagaa
    21181 gttagaaaaa caggtttagt tgattattta agaccagatg gaaaaactca agttactgtt
    21241 gaatatgaag gaagtaaagc tgtaagagta catacagttc ttatatcagc tcaacattgt
    21301 gaaacagtat caaatgataa aataagagaa gatttaatta atcatgttat taaggaagtt
    21361 ataccagcag aattacttga tgaagagact aaaatttata taaatccaac aggaagattc
    21421 gttataggag gaccacaagg agatacaggt cttacaggaa gaaaaataat aatagatact
    21481 tatggtggat attctagaca tggtggagga gctttctcag gaaaagaccc tacaaaagtt
    21541 gatagatctg ctgcttacgc agctagatat gttgcaaaaa atattgttgc ggctggtctt
    21601 gcagacaaat gtgagataga acttgcatat gctataggta tagctagacc attatctata
    21661 tttatagata cttttgggac aggcaaggtt tcagaagaaa aacttgttga attagtaaat
    21721 aaacactttg atttaagacc aggagcgata ataagagact tagatttaag aaagcctctt
    21781 tataaaaagg ttgctgctta tggtcacttt ggtagaactg atatagattt accttgggaa
    21841 agaactgaca aggttgagca gttaagaaaa gatgctttag gtgaataatt tttaactaaa
    21901 aagctgttag aatgtaattt tacgattact ttctaacagc ttttttatta tgagttaaat
    21961 atttttaata aatgttaatt tatcatcata taatgaataa acagtattta atctggtcaa
    22021 tacattttat ataatactct actaaattta tacatattat gggatatatg ttaaaggatg
    22081 gttaagaatg tgttaactaa tgttaaataa ttaaaaatat attgaaatat cacaataatg
    22141 agtctaatat aatcttaata aacttacact ttaaaaataa ttttaattgg gggggagtag
    22201 tgtatgtcag gcaaagtaaa tattaaaacg gttataagtt ttgctggagc ttatgttgct
    22261 actgtaatag gttctggatt tgcaactggt caagaaatac tgcaattttt tacgttttat
    22321 ggatatgcgg gtattatagg tggaataatc tctatggtac tattttcatg gtttggagca
    22381 gaagttatag ataagggaag agaattgaaa cttaaggagc caataaaaat ttatcaagta
    22441 tattgtggaa aatatcttgg aacatttttt gaatggtttg gtccattgtt tttatttgga
    22501 acattagtaa taatgattgc aggagcagga gctacattat cagagtacta tggattaaat
    22561 ccatatgtag gtaggattgg tatggcaatc gtttccctaa taactgtttc attaggtttg
    22621 acaaggcttt ctaaaatact aggtaatata ggacctataa taataatatt tacactttta
    22681 gtaggagcaa ttagtttatt tagcaatatt gatgctttat caaatgctgg aaatatggtt
    22741 gatagtttaa atattaaaac tgctacatca aatggatatt tctcgggagt actttatact
    22801 tgttacaatg taataatagt aataacattc ttgacaggaa tgggagcttc tgcagtaaat
    22861 aagaaagatg ctgtatgggg tggtatagta ggtggagtag cacttatggc agcagctata
    22921 atgatgaatt tggcattact ttcagacata ggaaatatat acacaaaaga gataccagca
    22981 ctttacttag ctgataagat atctccaata ataggtattt tattctcagt agtactttta
    23041 ctaggaatat acactacagc tgtaccattg ttatggtcag taacaaaccg atttgtagaa
    23101 gatgaccacc ctaagtttaa aattataaca atagtggtaa gtatattagc ttgtatagga
    23161 ggtttactgc catttgataa gttagtaggt acactatatc catatacagg ttacatggga
    23221 attttaattt tactatgtat gctttataga agaattacta aaactgaggg gtacaaggag
    23281 aataaatctg aaataagtta gtactgtaat atacttatga aatttaattt atactctaaa
    23341 tatagagtaa ttaaaataaa atagctattt aaatatggta cctgtatttc atggtaccat
    23401 attttatgga tgcagatttt ttttgtttct gataaaatgt ataatagttg gaaattacgt
    23461 taagtataaa aatataaaac ttttctacga ttatcatagt tttaatatat aaaattatac
    23521 taacatttaa atggagcgat tattagtatt tttaggagat aaaaatggaa aaattagaag
    23581 gaatgataag tgaaatagtc tttaaaaacg aagataatgg ttatacaata gctcacctgg
    23641 taaatgaaaa tgatgaaatt gtggttgtag gatgtatgcc tactttggca attggagaaa
    23701 gcatagaagt tgaaggaaag tgggtaaatc ataagattta tgggactcaa tttgaagtga
    23761 atagttttat gccagtaaca ccatcatcct tagaaggaat ttatgtttac ttatcttctg
    23821 gaatgataca tggtattggt gaaaaaatgg caaaaagaat aattgataag tttggtgtag
    23881 atacgttaga ggtgatacaa aattcaccag aaaaactaca agaagtagaa ggcataggga
    23941 gtaaaaaagt aaaacaaata gtaaaaagtt atgaagaaga cagagaattg agaaatataa
    24001 taatacagct atctcctttt ggaataacac caaattactg cctaaaaata tataaaaaat
    24061 ataaaagcag tgcaatagaa gttataaata aaaacccata ccaacttgct gaagatataa
    24121 gaggaattgg atttaaagta gctgatagta tagcaagtaa aattggtata gataaaaact
    24181 ctaaagatag aatttgtcag ggaatcttat acactttaaa taaatcttta agtaatgggc
    24241 atacatattt accagagcat gttctaatac aagattcgga aaaattattg gagctaaatg
    24301 gagaaataat aaaagagtgt gtaatgatgc tagtttataa tcaaaaaata catatagaga
    24361 aagtaaacaa tgaaaatctt atatacctta tgccatacta tttagctgaa aatggagtat
    24421 gtagtcaaat agtaaaatta tcacagtatg aatttgaaga cttaaaaata gatatagata
    24481 acgagattaa tgttttagaa gaagataaga aaattaagtt agcagaaaaa caaattttag
    24541 cagtcaaaga atctgttaat agtggagtat taattataac tggtggacca ggaactggta
    24601 aaacaactac cattaatgct atcatagata tatttgaaaa taatggtaaa agtgtgactt
    24661 tggcagctcc aacgggaaga gcagcaaaga gaatgagtga aacttctaat aaagaagcaa
    24721 aaactataca taggttgcta gaaatgggat tttcaacaga tgatgattta acttttttta
    24781 aggatgaaga agacccaata aattcagatg taataattgt tgatgaggta tcaatggtag
    24841 acattatctt aatgtacaat ttacttagag ctataaagtt aggaacaaga gtaattttag
    24901 ttggagatag tgaccaatta ccttctgtag gtgctggaaa tgtacttaag gatatgatta
    24961 attctaatat tataaatgta gttaaattaa atgaaatatt tagacaagcg caagaaagta
    25021 tgataattgt aaatgcccat aagataaaca atggagaacc actttatcta aatacaaaag
    25081 gtaaagattt cttttttata agaaaatcaa ctaatgaaga aattttaaat gaaataatag
    25141 gattggttaa tgaaagatta cctaaatttt ataaagttga taaattaaaa gatatacaag
    25201 ttttatcttc aatgagaaaa ggtgaattgg gagttactaa tctcaatata gagttacaga
    25261 agtatcttaa caaaaaagaa aaatttaaag ttgaagaaag tttttctaaa agattattta
    25321 gagttggcga taaggtaatg caagtaaaaa ataattatac aaaaaaatgg gaaacagaag
    25381 accaaaaaga aagtggagaa ggtatctata atggagatat aggatatgtt tatcacatag
    25441 ataaagataa gaaaactatt tatatattat ttgaccagac taaaattgta tcatacctat
    25501 atgatgaatt agatgaaatt gaccatagtt tttgtacaac aatacataaa agccagggaa
    25561 gtgaatttcc agttgtagta ttgccaatag cgtgggctcc tcctatgtta cttagccgta
    25621 atttattata tacagcagta acaagagcaa aaaaattagt tgtattagtt ggagatgtga
    25681 aatatcttga atatatgata aaaaataata gagttaatca aaggtattca aatttaggat
    25741 ataagctaaa taaatttaaa caagaagggt tgttgataga ataaatatga aaatatgcaa
    25801 tgataatttt attaagaatt ttaaaaatat gattaataaa tgtttggatt ttatatatcc
    25861 tgagaatata agttgcataa tttgtgataa atccataaag aaaacaaata cttattcaat
    25921 atgtaaaagt tgttttaaag aaatgaattt tatacaagat ggttgcatga agtgtggaaa
    25981 accaataata tgtcattcaa ttgaaaaaga atttattgaa gagtgtagtt attgttttaa
    26041 taaagacttt tattttgata aatcaatatc ttgtattgaa tacaatgatg tgacaaaaaa
    26101 gatggtgtta gggctgaagt ataaccaaaa aacctttatg gctaagtata tagctcaaat
    26161 aatgaaagaa aaattatatt tagaaaacat aaaatttgat tatatattat ttgttccact
    26221 tcataaaaaa aggctaaata aaagaggttt taatcaggca caaaaaatag catttaattt
    26281 aagtaaaata gtaaatattc cattattaga ttgcataagt agaaaaaaat atacaagaat
    26341 gctatataaa ttgaataaga aagaacgaaa agaagaatta aaaaatgtat ttgttgttaa
    26401 agaaaatgtt aaattaataa ataataaaaa tatactttta atagatgata tatttactac
    26461 tggtctaaca acaaacgaaa tttctaaact tttaaagtta tcaggtgcta ataaggtttt
    26521 tgtattgact ctacttacaa aagcaaatga taattatatt atggaataat cttatatatt
    26581 gacaatttta gtttaaaata ttaaaatgaa taagtgatat ttaggtctgt ggttgaaagt
    26641 ccaagccagt cgcaggcaaa gggatccacg taagttaaca ctaaaaattg ggttaatgat
    26701 catggtgcgg cttagaagta agacctaccg ataataaaat cgagagagag tagtagtgcg
    26761 gcgaacccaa gcgaaccctc cagtaggcga gtgtggggtc aaaaaccagg tcagctaaat
    26821 atcatattaa aagagagcta gcataccttt atgtatagct cttttttata tataataaat
    26881 ttaggttgag tcataaagag gtgatatgtt tgaataaaag acaagaaaat ttattaacaa
    26941 cattgctatt aaaagaagag tttataaatg ttaataagat tgccaatgat tttgagtgtt
    27001 cagaaaggac aataagaaat gattgcaaat atattgatga atggttatct actttttgta
    27061 atgcttatat caaaagaaaa cctaatattg ggattaaatt tagtggagat ggaacagaca
    27121 aaatactggt aaatcagaag ttgcgaggtg aagttaaaag agtaaatagt gagcttcata
    27181 agcaagttga gatattaaat ttactacttt gtaataataa aaaagttact ttaaatgatt
    27241 tatctaataa gttgtacata aataaaaata ttgtcagaga agaaataagt aaattatcag
    27301 tagcattaaa taattataat ttaaatatct cgactaaaaa aggcagtggt atatatattg
    27361 aaggggatga gaaagacata agaataatgt tagtatcatt cttatttaaa tatatagata
    27421 aatacagatt aaacatagat gaaatagagt acttccatat agtagatata ataattgtaa
    27481 aaaacataat gcttactata gaggaggata tggatataag acttacagat atatcattca
    27541 agcaattgat gctatttttg cttataaata taatacgaat aaggctggga aattctttaa
    27601 aagtatcaaa tactaagaaa gagcttaagc taaaaatgct tatattggat atagaagatg
    27661 aattgaaatc taatttatcc atagtattaa attatgaaga aaaattgttt atagaatctt
    27721 taatattggg tatgaacaaa caaataaata gtaataatat taagaaatct ttatatctaa
    27781 atgaagaatt agcaagttat acaaaaaaaa ttataaattt agtatcagaa gaatcaggaa
    27841 taaattttaa taatgatata ttactttatg aacaattaat atgtcatttg aatgttacaa
    27901 tgcatcaaat gaaaagcaat gtttacttag aaaatccact tttagataac ataaaaacta
    27961 aattttgttt tttatttaca gtaatatcaa gttcaataga aattgaatta aaaggaaaac
    28021 aaataacgga agatgaaatt ggttatctta cacttcactt tcaaacctct ctagaaagaa
    28081 aatacagcaa aagggaatca aataaaaagg caagtattgt atgtccattt agttttggag
    28141 taagtatgct attaaaagta aagatagaaa agagatttga taacataaaa attattgaaa
    28201 cattaagaga ggaagattta aaaagagata actttaataa gactatagat tttataattg
    28261 catttcaaga gtatgaaaat atccaaaaac caatttttat aacaacacca ttatttactg
    28321 atgaggatga gaataaactt aaagagttca caaacaaaat aaaagaaaaa gacacttcat
    28381 ataaacttat gaacagatta atgatgtctg attatttaat aagagagtta gaacatgatg
    28441 atatttatga agctataaca tatttaacta atttattaat tgaaaaacat tatgcagaaa
    28501 aagagtattt acaatctatt ataacaagag aaaagtcata tccaaccaat gttggaaaag
    28561 gaatattatt ccctcatgga gacatgaaat atataaaaca atctgtttta tgttttgcaa
    28621 ggttaaaaac accaataaaa attagaaatg gaaaggatat aaagattata cttttgttag
    28681 catataaaaa agatgatgat agaaaagtat ttagagaatt atttaaagaa atatctaatt
    28741 taacagagga tgaaaatatg ctagatattc taagtaaatg tgacattgaa aaggtaaaag
    28801 atattcttat ataaaattca tagtgaacta tggataagca caaaattttc catgtgatgg
    28861 aaaaagttgt gcatgttttt ttgtttttat aagatattat atgtttgtaa ggtataaata
    28921 atctaaaagt agagaagcaa cttaggagga aaaaatggaa gaaatagttt taaagattat
    28981 aattcatgct ggaaatgcaa aatctatgct ttatgaggca cttgactatg caaaggaaaa
    29041 cgattttaaa aaagcagatg aattaattga aaatgcaaat gaagaaattt taaaagcaca
    29101 taaagtacaa actgaactta tacaaaagga agctggggga gataaatcag atatatctat
    29161 tttattgatt cactctcaag accatttaat gacttgtatg agtgagagaa accttataaa
    29221 agaaatgatt atgttaagaa aagaaattca aaaactaaaa taaaaaatta agggggattt
    29281 aaaaaatgaa aagaaaagta tatttatttt gtagctttgg tatgtcaaca agtttacttg
    29341 cagataaaat gcagaaggta gcagatgaac acaatttacc aatagaagta gaggcatttc
    29401 cgatagcaga aattgacaaa atagttgaag aaaaaaatcc agattgtata ttattaggac
    29461 cacaagttaa atacatgcta aaggagttaa aaccaaagtt tgaagctcaa ggaaaactta
    29521 tagatataat caatgaagtt gattatggaa ctatgaatgg ggaaaaagta ttaaaacttg
    29581 ctataaagct aataaaatct aaacaagcgt agaattatag taaaaaataa taggaaatga
    29641 gggtggatag atgaatagat tggaaagaat acttatgcca ttggctgaaa aaataggtaa
    29701 aaataaatta ttaattgcaa ttagagatgg ttttttagta tcatcaccat tattaataat
    29761 aggttcatta tttttattag tagcaaattt tcctataaaa ggatggaatg aatttgtagg
    29821 tcaaattttt ggtccagatt gggctgtaaa attgcaacaa ccaactgtag caacatttga
    29881 agtaatgact ctacttgcag ttttagggat tggatattct tatgcaaagc aaatagatgt
    29941 agacccaata gcatcagcag cagttgcaat agttgccttt tttataataa caccatttgt
    30001 aataccatat acaccagaag gaacagaatc agtttacatg gtaacaggta taccactagg
    30061 ttggatgggt tctaagggaa tgtttgtagg tatgataaca gcaataacct ctgtaaaatt
    30121 atttgctgca gttgtaaaaa aaggatggac attgaaaatg ccagatggag ttcctccaac
    30181 tgtatcaaaa tcatttgcag cattaatacc aagtgcaata gttatggtag ttttcttttt
    30241 agttaagata gcttttgaag ctacaccata tggaagtata catgaattta tatttaagtt
    30301 tttacaaatg ccattattaa aattaggaaa tagtttaggt gctatggcaa tcgcttatat
    30361 attcttacat ttattctggt tctttggaat aaatggaagt agtgttgttg gggctgtgta
    30421 taatccaata ttaaaaattc tatcagctga aaacttagct gcttttcaag ctggttcaaa
    30481 aataccaaac ataataacag gtcaattcca agatatgttt gcgacatttg gtggagcagg
    30541 tagtacacta tcattagtca tagctatgtt attaatatgt aaaagtaaac gtataaagtc
    30601 aatgggaaaa ctatcaattt taccaggaat atttggtata aatgagccat tgatatttgg
    30661 tctaccaata atgcttaacc caatactatt gataccattt gcaatagttc caacaataaa
    30721 tataataata gcttacttct gtatgtcagc aggattagtg cctcttacaa atggagttca
    30781 gttaccgtgg acaactccaa ttatattctc tggtttttta acaacaggat ggcaaggtgc
    30841 agtcttacaa ttagtacttc ttatactagg aatatttatg tatataccat ttataaaaat
    30901 gctagataag caatacttga gagaagaaaa tgaaactgtt gaagaggaag atgatgatat
    30961 atcttttgat gatttagtat tataggagga agattatgaa gaaaatagtt atgatattgg
    31021 accaaataca agctggagct ggaggaaaag agaaaagtaa tatacctcct gctggaaaat
    31081 caagcccatt aggaccaggt gtaatgatgg agcaattttt aaatgaatct aaggtaatag
    31141 ctaccttatt ttgtggggat gaattttttg taaataatca agaagaagta acgagtaaga
    31201 tgatagctat ggtaaaaaaa ttaaaccctg atgtagtaat ctgtggacca agctttaatt
    31261 acgaaaactt ttctaagatg tcagcaatac tgtctaaaaa tataaatgat aaaacagata
    31321 taccagcttt tgcagctatg tcagaagaaa atatagatgt gataaacgaa tataaaaatg
    31381 acatatgtat agttaagact ccaaaaaaag gtggaattgg tcttaatgat tcattaaata
    31441 acatatgtaa attggcaaaa gcaatagcaa ataaagaaga tataactttg atgaaagaag
    31501 agttttgtta tcaataagag tattaattat tagcaagttt aaatattata ctatttataa
    31561 taagcataca ttagtaaaat ctatataatt taaggagggg aattttaaca tgtggggaat
    31621 gatagcgaca tggagaatgg ccctagaagg aataactgag gcatcagaac atttagaaaa
    31681 aggcatggaa gcaggtgatg ctgtagagct tgctgtaaga agagttgaag actatccatt
    31741 ctataaatcg gttggatatg gtggacttcc aaatgaaaat tgtgaggtag aacttgacgc
    31801 tgcatttatg gatggtaaca cattagatat tggcgcagtt gctggtataa gagattattc
    31861 taatcctgtc agcatagcta ggaagttaag ttatgagaag gtaaataatt ttttagttgg
    31921 aataggtgct gaagattatg ctcataaaaa tggatttgaa agaaaaaata tgttgactga
    31981 tagagcgaag ttacattata aaaagagaaa aaaagagact ttagacaaag gattaagccc
    32041 atatgctggt catgatactg ttggtatgat atctttagac aaaactggaa aaatgtgtgc
    32101 agctacatca acaagtggat tgtttatgaa gaaaagaggt agaataggag attctgcact
    32161 atctggttca ggattctatg ttgatagtag tataggagga gctacagcta ctggtttggg
    32221 agaagattta atgaagggat gtatatctta tgagatagtt agacttatga aagaagggaa
    32281 gcatccacaa gaagcatgtg atattgcagt aaatgaactt gataaacagc ttatcgaaag
    32341 aagaggaaaa gctggtgatt tatcattagt tgccatgaat aataaaggtg aatggggagt
    32401 agctacaaat atagctgatt ttactttttc agtagtaact aaagaagaat ctcctactgt
    32461 atatatttca aatagagtag atggaaagac tatacataag gttatatcaa atgagtggat
    32521 ggaagcatat gaggctagaa taaaagcacc aatagaagta agtgagtaaa gtagtggtta
    32581 cataattaaa atagagtttt ataataagga gatttttatg aatatcataa gtaataaaat
    32641 agatgagtta aaagaagatt tattaagtga tattatagat attgtaaaaa tacctagtgt
    32701 caaaggggag tcagaaaatg gcttcccttt tggcgaaaaa gtaggggagg cacttaataa
    32761 agcattagaa atatcagaaa aattgggatt taaggttaga aacttggata attatattgg
    32821 ttatgcagag catggagata gtgatgatta tgtttgtgta attggacatg tagatgttgt
    32881 acatgaagga gatggttgga agcatcagcc ttataaggga gaagaaacta atggaagaat
    32941 ttatggacga ggagtactag acaataaagg acctataatg tctgccttat atggattata
    33001 tgctattaaa gaattaaact taaaattaga taagagagtt agaataatat ttggtacaaa
    33061 tgaagaaagt ggatttgaag atataccata ttatttagaa aaagaaaaag cacctattat
    33121 gggatttaca ccagattgta aatatcctgt tgtatatggt gaaaaaggca tggcaaagat
    33181 tagaataaaa agtaaaatca attatgaaga agatgtatac ctaggtttta tagaaaatat
    33241 gtctgaaaat gtgcttgtta catataaaga gctgaatata gaaaatagtg atactatatt
    33301 agatataaag gtaaaatacg attttagtta taaattgaaa gatgtattgg atgaaataaa
    33361 agctagtttt ccaaatagta tcgatataga ggtaatttct aattttaatc ctgtatattt
    33421 tgataaagag agtaatcttg ttaaaaagct tcagctagct tatgaaaggg ttacatcatt
    33481 agatggaact cccgtaacaa ctaatggagg tacgtatgca aaagtaatgc caaatatagt
    33541 tccgtttgga ccttcatttc caggacaaaa aggaatagct cataatcctg atgaatacat
    33601 ggatatagaa gatataatat taaatgcaaa gatatttgca aatgcaatat atgaattagc
    33661 aaaggagtag ttatgttaga aataatagga atgtcagtag aagacgctaa aattatagaa
    33721 gattgtggtg cagatagaat agagttagtt agtgctttaa ctgaaggtgg attgacacca
    33781 agttttggtc ttatagaaag tgttgttaac agtgtaaaga tacctgtgaa cgtaatgata
    33841 agacatcatg cgaaaagttt tgtatacagt aaagaagata taagtataat gcaaaaagat
    33901 ataggtgtgg ttaaagagat tggtgctaat ggagttgtat ttggtgtatt ggataaaaac
    33961 aataatatag atgagaaaaa tctaaatgtt ttattgaaat gttgtgataa tcttgatgtt
    34021 acatttcata gagccataga tgaatcaaat acaatagata gtgttaaaat tttgaaggat
    34081 tatgataaaa ttacgaatat attaacatca ggagggaaag gaagtatagt tgataacata
    34141 caaatgataa agaatatgat gcttagttct aatcacataa aaattctttt aggtggaggt
    34201 cttaatttta ataatataga aaagataaag gaattgacta aggcaagtaa ttttcatttt
    34261 ggtacagcga ttagaataaa taacagtcct tttgaagata tagatagaca aaagcttaaa
    34321 caactcgtaa atataatttc aagatagtat atgaatataa aatacttagt ttaaataaag
    34381 agagtaagtt aattataatt tgggtatatg aatgttaata ggtattaaat atgaaattat
    34441 tgttgagtat gctttataac ttttaagtag ttttaaatat aaataatgaa agaaaagata
    34501 gattatggag gttttagagg gtttatattt attaaaaaat tataaaggta tattggcata
    34561 taatacacat aatttcctaa aattaccatc ttatgacatg aattcacata ttaataaaca
    34621 attaatggag atttttgtgg atagaaatag gagtaaaaat agctttaaat aaacaaaaac
    34681 aagagttatt aacagtttta tccacattat ccacaggtgt ttgtaataat attaatccac
    34741 ataagtatca atgtaactaa aaatgataaa taattctatc aattgaatag aaaaaatcat
    34801 aaaagtaaat aataaaaaga ggattatgag agttcgtgta gaatatatta ttaaagtata
    34861 aattttctta tactggcata attgaaggga gatgtattta tgaacataat tatatctgga
    34921 aaacaaatcg aattaactga tgcaataaaa aataaaatcg aaactaaact aagtaagtta
    34981 gataattaca ttcatccaga cactgatgta aaagttacag taagtgcgag aaaagcaaga
    35041 caaaaaatag aagtaacaat agctactgta aatggtccta taattagagc agaagatatg
    35101 caggaaaatt tatacacagc tatagatgtt gtatatgata aattaagtaa acaattaaaa
    35161 aaatataaaa agagattaca agataaacat aaagacaaca agagtataag attccaagat
    35221 gcagagataa atctagctga tgaacaagag gattatgggg atgaaaatca agaattagta
    35281 attcaaagac gtaagaaatt tagtgttaag ccaatgagtg aagaagaagc tgtattacag
    35341 atggaattaa taggacatga tttttatatg ttcaagaaca tagattcaga tgaaatagca
    35401 gtagtttata agcgtaataa tggtggatat ggtataatag aacatgaata aagcaattta
    35461 gatttaatca tattagctat cgataaataa attatcgata gctttttaaa ttaatattaa
    35521 aagtatagtt tttatggtaa aatgaataat agtacaataa agaacgagga gagatgtaaa
    35581 atatgagctt tatggataat ttattcaaca tggcagataa aaaagaatta aaaaagttca
    35641 ataaaactgt tgatataata gattcattag aacctaaatt tgaatctatg gcagactcgg
    35701 aacttaaaaa tatgacaaat atatttaaag aaagattagc aaatggtgaa agtatagatg
    35761 atatacttcc agaagctttt gcagtagtta gagaagtttc taagagagtg ttaggactta
    35821 gacactacag agttcaaatg attggaggta tagtactaca tcaaggaaga atagcagaaa
    35881 tgaaaacagg tgaaggtaaa actttggttg caactgctcc tgtttatttg aatgcgctta
    35941 ctggaaaagg tgtacatgtt gtaacagtaa atgattacct tgcaaaacgt gatagagacc
    36001 aaatggcaaa aatctatgag tttttaggaa tgagtgtagg cgttattata cacggtcaaa
    36061 atccaaaagt tagaaaagaa caatatgatt gtgatataac ttatggtaca aataatgaat
    36121 atggatttga ctatctaaag gacaatatgg taatacacaa agagcaaaga gttcaaagag
    36181 gattaaatta tgctatagtg gatgaggttg actctatact aatagatgag gctagaacac
    36241 cacttatcat atctggacct ggagacaaat caactcactt atattcagat gcaaacacat
    36301 ttgttttaac cttaaaacca gatgattatg aattagaaga aaaagataag gctgtatctc
    36361 taacagcatc gggtatacaa aaagcagaag tgtattttaa tgttgataat ataacagata
    36421 tatctcatac agaattatac catcatataa atcaagcttt aagagcacat gtaataatga
    36481 agaaagatgt tgattatgtt gctaaagatg gagaaatagt aatagtagat gagttcacag
    36541 gtagacttat gtttggtaga agatattcag aaggtttaca tcaagcaata gaggctaaag
    36601 aaggtcttaa aatacaaaga gaatctaaaa cactagctac tgtaacattc caaaattact
    36661 ttagaatgta caaaaaactt tctggtatga ctggtacagc aaaaacagaa gaagaagagt
    36721 ttaaagcaat ctacaaaatg gatgttttcc aagtacctac taataagctt atgataaggg
    36781 aagacttacc agactgtgta tacaaaagtg aaataggtaa atttaatgct gtagcacaag
    36841 aaataattga aaggcacaaa gtcaatcaac caatacttgt aggtacagta tcaatagaaa
    36901 aatcagaatt attatcacaa atactaaaga aaaagggaat aaagcacgag gtattaaatg
    36961 ctaagcatca tgataaagag gcagaaataa tagcacaagc tggtagactt ggagctgtta
    37021 caatagctac taatatggct ggtcgtggta cagatatagt tctaggtgga aatccagact
    37081 ttttaactaa gagagaaatg agaagaaatg gatttaaaga agaaatagta aatagagtag
    37141 atactccaat agaaggtata cctgtaaaag gtaatgaaat tctatttgaa gctagagaag
    37201 agtatgaaaa actatttgaa aagtttaagc aacaaacaca agaagaacaa aaacaagttg
    37261 tagaagcagg tggacttgct ataataggta cagaaagaca tgaatctcga agaatagata
    37321 accagttgag aggtcgtgct ggacgtcaag gtgacccagg gagttcaaga ttctatatag
    37381 gtcttgatga tgaccttatg agattgtttg gtagtgatag aatatctggt attgtagata
    37441 aaataggtct tgaagaagac atgccgatag aacatagaat attatctaag tctattgaag
    37501 gagcacaaaa gaaagtcgaa ggtaaaaact ttggaataag aaagcatgta cttcaatatg
    37561 atgatgttat gaataaacaa agagaaataa tatatgcaga gagaaaacgt gtattagaag
    37621 gtgaagattt acaagaacaa atacaatcaa tgactcattc tataatagaa gaagcagtta
    37681 ctctttatac acaagataaa ggatttgatg aagaaggatt caaagaacat atgtataatt
    37741 tgttcttgcc aaaaggtagc atagaaatac cagaaataga aaaacttaat ccagttgaaa
    37801 taacagaaaa agtttatgaa atagctatga agatatatac ttcaaaagaa gaacaagttg
    37861 gctatgagag aatgagagaa gttgagagag taatcttact tcaagctgtt gataaccatt
    37921 ggatagacca tatagatgct atggaccaat tacgccaagg tattggtctt cgtgctgtag
    37981 gtcagcaaga cccagttatt gcttataaaa tggaaggttt tgatatgttt gatgaaatga
    38041 ataaacatat caaagaagat actgtaagat atttatttaa tataacaata gaaactccag
    38101 tggaaagaaa agcagttgta gatgttgaaa atctatcttc tccatcagat ggaacacttc
    38161 caacaagtaa aactgttaaa aaagatgaaa aggttggaag aaatgactta tgcccttgtg
    38221 gaagtggtaa aaaatataaa aactgttgtg gaagataatc tcggaggatt aaatgttaaa
    38281 gttacaagag tatagacata gtatggagca gatggcttca aatttagaag aattgagggt
    38341 ttctctttga catagcttca ttgatgaaaa atatacaaat taatgaagaa aagatgaatc
    38401 aacaagattt ttggaatgat aatgaagtcg ctcaaagagt acttcaagaa aataaatctt
    38461 taaaagagac attagaagaa tatgaaagct taaaaagctt attagaagat atagaagtat
    38521 taattgaaat aggtttagaa gaagataatg attctgtaga aagagatata gaaaagtcca
    38581 tagaatcaat ggaagaaaaa ttatctgaaa tgaaaataaa gactcttcta aatggagagt
    38641 atgataaaaa taatgctata ttatctataa atgctggtac tggtggctta gatgctcaag
    38701 attgggctca aatgcttttg agaatgtata taagatggtc agaaagtaag ggttataaag
    38761 ttaagctttt agatataata tcagaccctg aagcagggat aaagacagct acaattcttg
    38821 tagaaggaac aaatgcttat gggtatttaa agagtgaaaa aggagttcat agattagtga
    38881 gaatatctcc atttgaccca tcaggaaaaa gacatacttc atttgcatct atagatgtaa
    38941 caccagagtt agatgaaaat atagaagtag aaattaatcc atcagattta aagatagaca
    39001 catatagagc ttctggagct ggagggcaac atgtaaatac gactgattca gcagttagaa
    39061 taacacatat ccctactggt gtagtagttc agtgccaaaa tgaaaggtct cagcatctaa
    39121 ataaggacag agctatgaga cttttaatgg cgaaattgat agaattaaaa gagttagagc
    39181 aaaaggaaaa aatagaagat atacaaggca aatactcaca aataacttgg ggtagtcaaa
    39241 ttaggtcata tgtttttcaa ccatataaat tggttaaaga ccatagaaca aatgctgagt
    39301 ttggaaatgt agatagtgta atgaatggaa atatagactt gtttattaat gaatacttaa
    39361 aaatgaataa aatagtttaa taaatatgca ctgtaattaa gatatatgta taagaaggtt
    39421 agtgcttaat atgcacttac agcacttagg ctgaggacga taaccctcag ccttcgttct
    39481 gttttgttat acttatgaaa aactaacatt aggagtgaat aaattggata taaatcaaat
    39541 cttaaaaaaa gagtttaatc ttagagatga acaaataaat aatacattaa aacttattga
    39601 tgaaggaaat acaataccat ttatagctag atatagaaaa gaaatgactg gcgaaatgag
    39661 tgatgttaca ctaagagaat tttatgaaaa attaatgtat ttgagaaatc ttcaaagtag
    39721 aaaagatgat gtagttagac taatagatga acaagggaaa ttaacagatg aaataacaca
    39781 aaatatagaa aaagcaaaaa cattacaaga agtagaagat atatatgctc catataaaca
    39841 aaagaaaaga actagagcaa caatagcaaa agaaaaagga ttagaaaatt tagcattgtc
    39901 aatactagag aataatttag ataatataga aatagaggca aagaattatc ttgatgaaga
    39961 gaaagaagtt ctttctattg aagatgctct aaaaggtgct agagatataa ttgcagaact
    40021 agtctcagat gatgctaaga ttagaaagta tataagagaa ttagctttaa gagaaggtat
    40081 gatagttagc aaaagtgcta ctgatgagaa atcggtttat gatatgtatt atgattatag
    40141 cgaagcagta aaaagtatgg ctccacatag agttttggca ataaacagag gagaaaaaga
    40201 aagcttccta aaggttaagt tggaaataaa taatgataaa gttttaaatt acataataaa
    40261 cgaatatgta aatgataaaa atttcaaaaa taaagaagaa atcgtaagtt caatagaaga
    40321 ttcttataaa agattaattt tcccatcaat agaaagagaa ataagaaatc atttaacaga
    40381 aatagctcaa gaaagagcta taagtgtatt tggaaagaat gttaaaagtt tattgcttca
    40441 accaccagtt aaagacaaag tagttatggg atttgaccct gcatttagaa ctggatgtaa
    40501 aatagctgta gtagataaaa atggtaaact actagattat acaacagtat atccaacaga
    40561 ccctcaaaat gatgtagagg gagcaaagaa agttttaaaa ggattgattg aaaaatacga
    40621 tatagatata atttcaattg gaaatggtac tgcatctaga gaatcagaaa cttttgtatc
    40681 agaaatgata aaagaaatag atagcgaagt gcagtatgtg atagttagtg aagcaggagc
    40741 atctgtttat tcagcatctg aacttgcaaa tgaggaacat ccagatataa atgtatcaat
    40801 aagaggagct atatctatag ctagaagatt acaagaccca ttagcagagc ttgtaaaaat
    40861 agaccctaaa agtattggtg ttggtcaata tcagcatgat ttaaacaaaa agagattaga
    40921 agaagtacta gatggagttg ttgaagattc tgtaaatagt gttggagtag atttaaatac
    40981 tgcatcatac tccctactag aacatgttgc aggaataagt aaagctatag caaaaaatat
    41041 aatagcatat agagaagaaa atggagattt tacgtcaaga gcacaattga aaaaagtaaa
    41101 aagactagga cctcaagcat ttactcaatg tgctggattt atgagaatct tagagggtaa
    41161 aaatccacta gataatacag gtgtgcatcc tgaaagttat gatatatgta aaaaaatgat
    41221 tgagataatt ggatattctt tagatgatgt aaagaataag aatataggtg aaatagatga
    41281 aaaaataaaa gaaattggct tgagggaatt aagtgaaaaa ctggaagtag gacaagttac
    41341 gttaaaagat atcatagctg aaatcaaaaa accaggaaga gacccaagag aagaaggtat
    41401 aaaacctata cttagaactg atgtcttaaa aattgaagat atacaagagg gtatgacttt
    41461 aaaaggtaca ataagaaatg ttgtagactt tggtgcattt gtagatattg gaataaaaaa
    41521 tgacggatta gttcataaat cagaaatgag taatagtttt gtgaaagacc ctatgagtat
    41581 tgttacagta ggtgatatag tagatgtaaa agtcattgga attgacttaa acaaaaaaag
    41641 agttgcctta tcaatgaaaa aatagataag tttatgtatg gttatagtac ttagattcta
    41701 tatttgtaaa taagattact cgagtgtata aatgttaaaa atttgtaaaa gtatatagta
    41761 agaaaatata ttaaaataag aacgatagtc ttgaaaatca ggaggaaaat gaatgatttt
    41821 tataaaaaat ggtaaaataa ataccataac aaatgggata attcatggag atatacttat
    41881 agatgaagga aaaataatag aaattggaga agatttgata gctcctttag atgtagaggt
    41941 aatagatgct agtaataaac ttgtgtttcc tggatttata gatgctcata ctcacttagg
    42001 tttatgggaa gatggtatag ggtttgaagg agcagatgga aatgaagaga ctgacccaat
    42061 aacaccacaa ttaaatccta tagatggaat taatccaatg gatagaactt ttaaagaagc
    42121 ttttgaaggt ggaataactt ctgtttgtac aacccctgga agtgcaaatg ttatgggtgg
    42181 tcaatgtata gctataaaga catgtggaaa aagaattgat aaaatggtaa taaaaaatcc
    42241 agttgcatct aaaattgctt ttggagaaaa tccaaagagc tgttatggtc aggatgataa
    42301 atctcctcaa actagaatgg ctatagctgc tttacttaga gaaaacctaa aaaaagcaga
    42361 agaatattta gaggatattg atatgtatga gagtcatgat gatgaagact gtgaaaaacc
    42421 tgaatatgat ataaaaatgg aaagtttaat accagtttta agaagagaaa ttccttttaa
    42481 ggcacatgct catagagcag atgatatgtt tacagcaata agaattgcaa aagaatttaa
    42541 tttaaagctt acattagacc attgtacaga aggtcatctt attgtagatg aattagtgga
    42601 agaagggttt cctgtaatag tagggccatc tttgtcagaa aggtctaaat ttgagcttag
    42661 aaatttgaca tttaatacag caggaatact ttcaaatgca ggtcttgatg tatgtataat
    42721 gacagaccat ccagtaatac cagttcaata tcttccaata tgtgcaggta ttgcagtaaa
    42781 acatggtatg aaagaagaaa aagcaataga atcaattacg ataaatccag ctaaggcttt
    42841 aggaatagaa gatagagtag gctctataga agttggtaaa gatgcagacc tagtaatttg
    42901 ggataattct ccactagaga ttcaaagtaa tgtactttac actataataa atggcaaagt
    42961 agtgtatgag aaaaaataac ttgacaatta ttaaaaaatt aactaatata agagtgtaag
    43021 gaaaattaaa aaatcaaaga agatcttcgg ggcagggtga aattccctac cggcggtata
    43081 gcccgcgaac tacttaggtg gttgatttgg tgagattcca aagccgacag taaagtctgg
    43141 atgagagaag atatttatgg aagataaata agtttatttt agtttgttta aactatatcc
    43201 ataaaaactc gaagataggg tcttcgagtt ttttgttttt cctaaaaaat ataaacatat
    43261 tgattagggg gaaaataatt atgcaaaaca cagcaaaaag aggcaatatg atttcaacaa
    43321 gaactttagt agtaatgtca attttatctg caatatctta tttattaatg tttattcaag
    43381 caccaatacc aggtatattt ccagatttct taaaaattga cttatcagat attccagcaa
    43441 tattcggagg aatgtctcta ggaccattag ttggatttgg aatagttgta gtaaaaaatg
    43501 tgttacaagc aataacagca acaacaactg gaggaatagg agaatttgca aatatattaa
    43561 ttggtggttc ttatgtaatg atactttgtt attcatataa gagaagtgga gatttaaaag
    43621 gtgtactgat tggttttgta ttaggaacaa tatctatgac tataattggt tcattagtaa
    43681 attattttgt aatgttgcca ctttatggtc aacttatggg actagatgct ataataggtt
    43741 taggttcagc tataaatcct caagttcatg atttattcac ttttgtaatt tggatgatag
    43801 ctccatttaa tgtgctgaag gctgttataa tatctttagt aacattgcca ctttataaaa
    43861 aaatgggaaa tataataaag aaataattag acaagaaaat ataaaattaa atgtattgaa
    43921 tagccataat ttatttataa ct
//
LOCUS       DAEOSP010000010        43480 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.010, whole genome shotgun sequence.
ACCESSION   DAEOSP010000010 DAEOSP010000000
VERSION     DAEOSP010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43480
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.010"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(471..647)
                     /locus_tag="KRH54_000407"
     CDS             complement(471..647)
                     /locus_tag="KRH54_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578086.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
     gene            1222..2244
                     /locus_tag="KRH54_000408"
     CDS             1222..2244
                     /locus_tag="KRH54_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578087.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFIAPVLETESYYID
                     KEKGRIEDTRSGIDVGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            2258..3145
                     /locus_tag="KRH54_000409"
     CDS             2258..3145
                     /locus_tag="KRH54_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8578088.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSIVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            3151..3924
                     /locus_tag="KRH54_000410"
     CDS             3151..3924
                     /locus_tag="KRH54_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8578089.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIVFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            4036..4761
                     /locus_tag="KRH54_000411"
     CDS             4036..4761
                     /locus_tag="KRH54_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8578090.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            4765..6168
                     /locus_tag="KRH54_000412"
     CDS             4765..6168
                     /locus_tag="KRH54_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8578091.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRNIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            6179..7003
                     /locus_tag="KRH54_000413"
     CDS             6179..7003
                     /locus_tag="KRH54_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBG8578092.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(7328..7948)
                     /locus_tag="KRH54_000414"
     CDS             complement(7328..7948)
                     /locus_tag="KRH54_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG8578093.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            8214..8546
                     /locus_tag="KRH54_000415"
     CDS             8214..8546
                     /locus_tag="KRH54_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8578094.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            complement(8666..9307)
                     /gene="pcp"
                     /locus_tag="KRH54_000416"
     CDS             complement(8666..9307)
                     /gene="pcp"
                     /locus_tag="KRH54_000416"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBG8578095.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(9328..10260)
                     /locus_tag="KRH54_000417"
     CDS             complement(9328..10260)
                     /locus_tag="KRH54_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBG8578096.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            complement(10282..10947)
                     /locus_tag="KRH54_000418"
     CDS             complement(10282..10947)
                     /locus_tag="KRH54_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBG8578097.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            complement(11519..11995)
                     /locus_tag="KRH54_000419"
     CDS             complement(11519..11995)
                     /locus_tag="KRH54_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HBG8578098.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            complement(11998..12402)
                     /locus_tag="KRH54_000420"
     CDS             complement(11998..12402)
                     /locus_tag="KRH54_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBG8578099.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            complement(12377..13006)
                     /locus_tag="KRH54_000421"
     CDS             complement(12377..13006)
                     /locus_tag="KRH54_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8578100.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     regulatory      13372..13415
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            13516..14484
                     /locus_tag="KRH54_000422"
     CDS             13516..14484
                     /locus_tag="KRH54_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBG8578101.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     gene            14486..15049
                     /locus_tag="KRH54_000423"
     CDS             14486..15049
                     /locus_tag="KRH54_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG8578102.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            15057..15746
                     /locus_tag="KRH54_000424"
     CDS             15057..15746
                     /locus_tag="KRH54_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG8578103.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            15873..16601
                     /locus_tag="KRH54_000425"
     CDS             15873..16601
                     /locus_tag="KRH54_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG8578104.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAQDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            16615..17289
                     /locus_tag="KRH54_000426"
     CDS             16615..17289
                     /locus_tag="KRH54_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG8578105.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            17315..18151
                     /locus_tag="KRH54_000427"
     CDS             17315..18151
                     /locus_tag="KRH54_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578106.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            18167..18838
                     /locus_tag="KRH54_000428"
     CDS             18167..18838
                     /locus_tag="KRH54_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8578107.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLINQNIY
                     DLVILDLMLPNINGFDILKKMRINNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            18828..20060
                     /locus_tag="KRH54_000429"
     CDS             18828..20060
                     /locus_tag="KRH54_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8578108.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            20210..20527
                     /gene="trxA"
                     /locus_tag="KRH54_000430"
     CDS             20210..20527
                     /gene="trxA"
                     /locus_tag="KRH54_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG8578109.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            20544..21422
                     /locus_tag="KRH54_000431"
     CDS             20544..21422
                     /locus_tag="KRH54_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG8578110.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            21692..22060
                     /locus_tag="KRH54_000432"
     CDS             21692..22060
                     /locus_tag="KRH54_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG8578111.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            22063..22434
                     /locus_tag="KRH54_000433"
     CDS             22063..22434
                     /locus_tag="KRH54_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBG8578112.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(22579..23034)
                     /locus_tag="KRH54_000434"
     CDS             complement(22579..23034)
                     /locus_tag="KRH54_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578113.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(23073..24338)
                     /locus_tag="KRH54_000435"
     CDS             complement(23073..24338)
                     /locus_tag="KRH54_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBG8578114.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSIAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
     gene            24808..26259
                     /locus_tag="KRH54_000436"
     CDS             24808..26259
                     /locus_tag="KRH54_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG8578115.1"
                     /translation="MDFFNGILTSINNAVVYVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(26478..26939)
                     /locus_tag="KRH54_000437"
     CDS             complement(26478..26939)
                     /locus_tag="KRH54_000437"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBG8578116.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(27044..28237)
                     /locus_tag="KRH54_000438"
     CDS             complement(27044..28237)
                     /locus_tag="KRH54_000438"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBG8578117.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(28260..28925)
                     /locus_tag="KRH54_000439"
     CDS             complement(28260..28925)
                     /locus_tag="KRH54_000439"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBG8578118.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSNNHKSNIDKDFLFK
                     NGF"
     gene            complement(28959..30071)
                     /gene="ribD"
                     /locus_tag="KRH54_000440"
     CDS             complement(28959..30071)
                     /gene="ribD"
                     /locus_tag="KRH54_000440"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBG8578119.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKASNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(30203..30324)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            30513..32996
                     /gene="recQ"
                     /locus_tag="KRH54_000441"
     CDS             30513..32996
                     /gene="recQ"
                     /locus_tag="KRH54_000441"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBG8578120.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      33085..33196
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            33338..34639
                     /gene="thiC"
                     /locus_tag="KRH54_000442"
     CDS             33338..34639
                     /gene="thiC"
                     /locus_tag="KRH54_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBG8578121.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            34693..34887
                     /gene="thiS"
                     /locus_tag="KRH54_000443"
     CDS             34693..34887
                     /gene="thiS"
                     /locus_tag="KRH54_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBG8578122.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            34880..35683
                     /gene="thiF"
                     /locus_tag="KRH54_000444"
     CDS             34880..35683
                     /gene="thiF"
                     /locus_tag="KRH54_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBG8578123.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            35746..36516
                     /locus_tag="KRH54_000445"
     CDS             35746..36516
                     /locus_tag="KRH54_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBG8578124.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            36533..37636
                     /gene="thiH"
                     /locus_tag="KRH54_000446"
     CDS             36533..37636
                     /gene="thiH"
                     /locus_tag="KRH54_000446"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBG8578125.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEVISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            37637..38326
                     /locus_tag="KRH54_000447"
     CDS             37637..38326
                     /locus_tag="KRH54_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG8578126.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKEFRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            38514..39908
                     /gene="yfcC"
                     /locus_tag="KRH54_000448"
     CDS             38514..39908
                     /gene="yfcC"
                     /locus_tag="KRH54_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBG8578127.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            40425..41792
                     /locus_tag="KRH54_000449"
     CDS             40425..41792
                     /locus_tag="KRH54_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBG8578128.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            41817..42425
                     /locus_tag="KRH54_000450"
     CDS             41817..42425
                     /locus_tag="KRH54_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBG8578129.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYKKLTKV"
     gene            42473..42844
                     /locus_tag="KRH54_000451"
     CDS             42473..42844
                     /locus_tag="KRH54_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBG8578130.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            43023..43361
                     /locus_tag="KRH54_000452"
     CDS             43023..43361
                     /locus_tag="KRH54_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBG8578131.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
BASE COUNT        16695 a         4823 c         6998 g        14964 t
ORIGIN      
        1 ataaaaaact tatataaata aaatgcttgt ttttcttgta ttatttatat aaatattgaa
       61 atttgtctag ttatacgttt ttattgacca gttatgcagt tgatattgaa tattctaaat
      121 attggtgtta ttttataggt ggtttaaaca tattaggggg attttatata tgatttgtat
      181 gatttataga gaatgtataa aatagtaaag gtttagagcc atgtttagtg gattgcagaa
      241 tatgcatata gaatgattct tgtaaattaa gttagacata ggaaatatat aatttgtatt
      301 ggatgattga ttttatggtg tatgctttta acttctataa tgggaaagta aagatgtact
      361 cccaatacat ctttacttta gcagaagtta tagttactta taaagtacaa aaaattatat
      421 atttattaga gtagataaat ctaaaaaggt ttttctagaa aaatttagtt ttaattgtat
      481 ctacttacta ataataagac ttgatgtaag caggtttcaa ctaccgtttc tccatattct
      541 gatataaaat catctaactg tgtatctaaa aaattacaat aatcatatga actactgatg
      601 taagcagtta gtatatgtgt acatttgtct atttctccaa taaacatatt atccatcccc
      661 ctaaaaaatt tagtaaaaat aaaaagtcgc attctattag tcaattaaca taatttataa
      721 aattttttag tactagtttc tataacttaa ctccttaagg ttaaatattc ccagttttac
      781 caatttagtt ttattctaca agtatataat actagaagat gctccattat tcaacatgct
      841 tgtttgagaa tagaaaatat tctaaaatta taattgtttt taaaaataag attcaagata
      901 aataaagtta tatgcaacta tttaataatc tttccataat aaggacagta ggttctattc
      961 ttaaaaaact ccatctttaa atttaaagaa taactacaat ttgaacaaca ataaaatttc
     1021 caatcaataa aaatatcaaa aataataaaa ataattgaaa atgtataatt tcccagaaaa
     1081 ataataaatg taactccaat aataaacaca cctataaatt ataaaataat ttaagcttta
     1141 tttaaggtta gtattatatt tatataagat ttaagtttta atatatatgt gtaattaaaa
     1201 tgatttagga ggagaaatat attggtaaat tgggaaataa aaaaattggc taaatctaag
     1261 agtatgttta tatctctagg gatattagtg ttaatactaa tgattagtat ctttattgcg
     1321 cctgttttag aaacagaaag ttattatata gataaagaaa aaggacgtat agaagatacg
     1381 agaagtggta tcgacgtagg taatgagaaa tttcaaaata agattaatgt tttaaagcag
     1441 ttttcaaatc aaacagatac aggtgaattt tctaaaaaaa ttgaattaat gtcaaagaaa
     1501 aaacttgata atctaagtgt gaatgaatat aaggatatta gtttttggaa agtatttaac
     1561 tatagagtta caaattcact tataaatgta ggaatgttaa tcattatatc tataattata
     1621 tccaatattt acgtagatga agtcgttact tcggtaaaag atatcatact atcatcaaaa
     1681 gagaagaaaa aagcattaaa gtctaaaata tttgttgctt tattagttcc attagtaatt
     1741 tattctgttt atttaatggg aatatttatt ataacttgta tgcaacaagg agtacctgtt
     1801 aatggagact tagaatcttt tagaatagta gacaatgtag ttgcattgaa aactaattta
     1861 agtataacaa agtatgtaat attaaatata ggcatagagc ttttgatgtt tgaaggatgg
     1921 gccatgatag ccatatttgg gtcttttata agtaagtctt caatatcttc aataagtttt
     1981 tttatattta ttatatctat aactaagata atgtcggtaa tccatatatt acctaaaaaa
     2041 ttactttcag ttgtatcaaa tgtaaattat tatgatttga tatttggttt taataaaata
     2101 ataggaaatt atctaggtga tattatgata tttaaagtga atatagacat tgtcaatata
     2161 gcaataggac ttttgatggc aatattgata ggtgctatgt cattatgttt tttagtaata
     2221 aaatcagatt atataagtag ataaaagggg agttaagatg aataaattag aaatagaaaa
     2281 tctttcaaag atatatggta aaaaagtagc aaacgacaag attacagtaa ctctagaaaa
     2341 tggagtttat ggtcttctag gaccaaatgg agcaggaaaa tctacattaa tgaaacaaat
     2401 aacaacgttg ataaaacctg atgaaggtca gatactttat aatggtgagg atatatttaa
     2461 aatggatgct gattatagaa atattttagg atatttgcca caagaatttg gagtttataa
     2521 aaattttaca gcaaaagaat ttttacagta tattggagct ttaaaaggtt taaggggaaa
     2581 atatttaaat agtaagatag gagaattatt agatttagta ggtctttatg atgtgagaaa
     2641 taagtctata ggaaaatttt caggaggtat gaagaggaga gttgggatag cacaagtttt
     2701 actaaacgac cctaagataa tagtccttga tgagccaaca gcaggtcttg acccacaaga
     2761 aagagctaga tttaggaatt taatagcaaa aatgtctaga gataagataa taatactttc
     2821 tactcatata atttccgata tagaatcagt tgcaaaggaa acaataatga ttaaagatgg
     2881 caaactccta atgaaaggaa cacacaaaga catattaagt cacatggaaa ataaagtata
     2941 taatgttcaa gttaaaaatg aatgtgaagt tgatgatata caatctaaat ataaggttgt
     3001 aagtattcaa agtgatattg attcaatagt acttaggatt gtttcagagg atattccaag
     3061 cgaggataat atacaaccag ttcaagcaaa attcgaagat gtatacatgt tttattttga
     3121 tttagaagat gcaagggagg tgtagtagaa atgatgtatt tagttaaagc tgaaatgata
     3181 aaaatattaa aatcaaagag tgtttggata atttggctgt ttttaatagt ttttggattt
     3241 tttctgatta gaaagtttga tgttttagat acttatgcag atatatttta caagatagaa
     3301 ggctcaatac ctctaatagg tttgataatg tttataataa cttctggaaa ttacattaaa
     3361 gaatatgatt ctaatatgac aggtttaata aatacaacta aaaagggaaa aaaatcagta
     3421 gtattatcaa aactagtagc taatggatta attttatcta ttattaatat ttcttttata
     3481 ttgttagtag gaattagagg ttttagtttt tttgatttta aaaatttaaa tgaatcgatt
     3541 cataacttat ggtactttgg aaatagtaat ttaaatctta cagtaataca aatgtatata
     3601 attgttatat tgactactat ttttgcttct tttatatttg cacaaatagg cttaacactt
     3661 tcatctatat ttaaatcggc tgtaattcca tttatactag gtggattgat aatggcaatt
     3721 ccatacttta gtgtaggttt tataccagat aaagctataa aatttatgtc agtgactcca
     3781 aattggatta tgatgagcca acaaatggtt aagtataatg taccaagtat tttgatagtt
     3841 ttttctatag taatctcaat tatattaatg atagtgttaa ctaaaataac atatgaaaat
     3901 tttacatcaa gcaaaaggtt ttaactaaaa ataatagtaa ttttatttat attttagttt
     3961 aattaaaaat aagataaatg aattttagat aaaatatatg ctaatatata tgtaaattat
     4021 tacaggaggt tgtttatgga aaatcgtgtt ttaataatag atgatgaagt agaaatattg
     4081 aagcttttag aaactgtcct aaaaaaagag ggtcttaata atatatatac tgcaaaaact
     4141 aaaaaagaag gattagaatt atttaaaagt atcaatcctg atttgattgt attggatatt
     4201 atgttaccag atggcgaagg ttatgatatt tgtaaagaaa taagaaaaac ctccaattct
     4261 ccaataatat ttttatcagc taaaaccgaa gaattagata agttacttgg actagcgatt
     4321 ggaggagatg attatgttac aaaacctttt agccctaagg aagtggcttt tagagttaaa
     4381 gcgcatttga gaagattaag ttattttagt gatgctcaaa acgaaagtaa aaatctaaat
     4441 aataatgaag aaaaaattat atcatttggc ccatatatat taaatgaaag tagggcagaa
     4501 cttataaaag atggaaaatc tataggactg tctgcaaagg aactaaaaat cctatcttta
     4561 tttgcacata atcaaaatca aattataagt aaagaaaaac tatgggataa agtgtgggga
     4621 gaagattatg ttggatttga caatactata atggttcata taagaaaaat tagggaaaaa
     4681 ttagaggata atccttcaaa acctgaatat attctgacaa taaaaggtct tggatataaa
     4741 ttagcagtaa aggaagatta gaatatgaag tggaagatta ctagaaattt tatatttaca
     4801 attgtgtttg tagctatatc agtagtaatt ataaatatca tatctatatt gtatgtgatt
     4861 tcaactaata gcttttttaa agttgtagat tctggaaata atcctgaaga atttgcacgt
     4921 tcttttgaaa aagatttata tgaaaaagat ggagaattta aactatctaa gattggagct
     4981 gaaaaattag aaaaatcaaa ttcctggata caggttttaa atgatttagg agaggaagtt
     5041 tatggagtaa atgtgccaaa ggatactccc aaaaaatata caccctttca aatggtgaat
     5101 aattacaagt atatagaaac aaagtatgtt aattttgtac tagaaaaata tttaaacaaa
     5161 aagcatttaa acataatagt aggtatacct agtagagata tatcaagaat aattttaacc
     5221 tattctcaaa ataacattaa aaaaacttta aataaagtta tcattataac actagtaatt
     5281 gatagcgtgg tagctttagg cgtaggatat ttatttagta gaaaacttac aaaaccaatt
     5341 tcaagcgtac tatggagtat tgagactatg gctaatggaa attatagttt atatcttaag
     5401 gacagaggta tatatgaaga agtttttaaa aatataaata tgttagctga tactttaaga
     5461 gtaaatgaag ttgagagaaa agaaaatgaa gagttgagag aggaatggct ggcaaatata
     5521 acacatgata taaagacacc tctagcatct atacaagggt atgcagagat aattaatgac
     5581 aaagattatg agtttgaaga agatgaaata caagagtata cagaaattat atataataag
     5641 tctaagtata taaaagattt agttgatgat ttaaatttat ctactcgatt aaaaaataat
     5701 actatagttc tggacaagaa aaaaataaat ttagtcagtt tagtcagaaa tataataatt
     5761 gatattttaa atgataatag atataaaaat aggaatattg aatttgaaag caatgaagat
     5821 ttaattgaag tatatgtaga ttcaatctta ttcagaaggg ctataaccaa tcttatattt
     5881 aattcaatag ttcataatag cgaaggaact ttgataagtg tagaaattgt aaaaaaagat
     5941 aatatagaaa ttataataaa agataatgga attggaataa gcaaaagtga tttgaaacat
     6001 atttttaaaa aatattatag aggtactaat acaggtgaga tgcataaagg ttcaggtctt
     6061 ggtatggcta tttctaaaga aataatagag attcataaag gtaaaattta tgtaagtagt
     6121 gaaattggaa taggaacaaa aattattata gagataaaac aaaattagga gatatgatat
     6181 ggattttttt aaaaaaggta taagtgggtt tactattaag attttagcat taatatttat
     6241 gacatttgac catatagcag catttatgcc acaaacaatg caaataccaa tttggtttca
     6301 ttgggtaggc agaatatcag caccactgtt tatattcatg gctgttgaag gattttatca
     6361 cactagtaac agaaaaaaat atataagtag attgtatatt tggtctgtta ttatggctat
     6421 aggaaatcaa ataattaata atgtattttc acatccagaa ggagctataa ttataaataa
     6481 cattttttca acattatttt taatagcgat ttatttacaa gcaatagagt ttataaagaa
     6541 atttagaaaa gagaaagaaa ttaaatattt tattatagga ttattgatga taatcattcc
     6601 tataatttta ggaatattta cagtagcttt actttttaaa gtcactaaca gagttattgc
     6661 tttatttatg attttggtac cagtaccatt tttagtagaa ggtggaccaa tttggattat
     6721 acttgggatt attttttatt tgtgtagagg gaaaaagttc agtttgagta tttgttatgt
     6781 tttaatgtgt atatttatat ttacaacaat gtcaaatgga gattatagtt taaaaaattc
     6841 tattttacaa aattaccaat ggatgatgat tgcttctcta cctttaatgt tactttacaa
     6901 tggagaaaaa gggaagagta tgaagtattt attttattta tactatccaa tccatgtata
     6961 tatattgtat atcttaggaa tatacttaat aaatggtttt tgattgtaag tgatattttt
     7021 aatacaaaat gaacaatatg aggattgttt taggtctatt aaaattgtta tttagatgag
     7081 atttgtagaa tatttaaatt tatgagcaga aattcagtta ttgtaatttc aataacatta
     7141 gaatataata ttataattta tttagaaata tatgatgtta tacatggttt ggttattaat
     7201 gtaataataa aacagaggtt tgatataaaa ttcaatacct ctgttttagt gctttttatc
     7261 ctacatttat gcttatttta tttataaatt agtgttttgt tttaattaat attagtgcat
     7321 tttttattta ttttataaaa ttagtcataa ttgtttcttc atattgtggt tctttaattc
     7381 cagctttttt acctgctgat atacacttaa gaagccatgc catattttct cctagagttc
     7441 gcatagtctg taatccttct tcatccttca taacatcctc tggagtaaag ccatgtacct
     7501 gattccaata ctgtgatgat acaataggca tattgctgat tgaaaaatat ttatttagtt
     7561 ggtcaaatgt tgcacttgca ccaccacgtc tacatgacac aactgaagca cctaattttg
     7621 tagccatgcg acttccagct atgaaaaata gacggtcaag aaaagaagtt aattgaccag
     7681 ttgcactaga aaagtaaaca ggagaaccaa taataatacc atcaatatta tcaagatctt
     7741 caatcaattc attaacttta tcatttcgaa tacattttcc agtttcaaaa caggatgcgc
     7801 aactaataca tcctgggatt gtttctttac ctaaatacag tatttctgtt ttaatatcgt
     7861 gtttactcaa tactttagta atctcattta gtgctgtata agtacatcca tattggtttg
     7921 ggctaccgtt tatcaataaa actttcatta taaatttcct cccatgaatt ttatttaata
     7981 gattttctaa gagatattat tttttataaa ataggatttt catgaatatt atcaatttat
     8041 atagaaatgt ctaatcttag aatcctattt aaaataactt tttttttagt aaatacacct
     8101 cctattataa aattgacttt caattattct tacactataa ttttattata aattattcta
     8161 aaaacaagta cgcacatttt tgttcaatac taaccaaaag gagagtgtaa tatatgagta
     8221 tagactgtat atcaaataca gatttaagtg aaacaggatt tagttataca ctttcactga
     8281 ttagtggtaa atataagatg ataattcttt actgtcttgt agaatttgaa gttgtaagat
     8341 ataatgcatt aaaacgttat ataaatacta tctcctataa aactttgagt ttatcattaa
     8401 aagaattaga agcagataac ttgattattc gtactgaata tcctcaaata cctcctaagg
     8461 tagaatatag tctatcagaa aggggaaaat ctctgattcc aattttagat gcaatgtgtg
     8521 agtggggaga gaggaataga ccttaatttt gtatctgtag ataccataaa aagactgtga
     8581 gtaaaattgt ttgaataatc tactcacagt ctatatttaa attaatagtt agtattttta
     8641 atttataaaa atactaaaaa atttattatg aaatttcacc accaatttct tttacatctt
     8701 ctttatatat aatacaagct tctatagcta attctaagcc ttttactatt tcttctaaag
     8761 acatgaatgg tgtatttttt ttatctatta cttgtgaagt tgtatatggg atgtgtataa
     8821 atccacccct tatattagga tatttcttgt ctatcatata aagaattcca tacatcacat
     8881 gattgcaaac aaatgttcct gcagtgtttg aaacactagc tggaatttta ttttcattca
     8941 tatgttttac catagctttt acaggcaatt ttgaaaaata tgcaggttca ccatcttctt
     9001 ttataatagt atctataacc tggttaccct cattatcttt tattctaaaa tcatctatat
     9061 ttatagcaac tctctcaggt gtaatgtcaa atctacctcc agcttgtcca actgatatta
     9121 ctatatctgg attgtgctct tgaatagctt tatctatagc ttcaacagat tttgtcataa
     9181 ctgttggtat agttatcttt atgatttctg caccatttat attattattg accctttcaa
     9241 ctgcttcttg agctgggttt attggttctc caccaaatgg gtcaaatcct gttaacaaaa
     9301 ttttcattat tttacctcct aattatatta aaatcctaaa aagtacatta ctaatatgtg
     9361 ggctataatt aataccaaag caactggagc ttgatatttt ataacaatat aatcattttt
     9421 acattctagc aaggctgctg gaactatatt aaaattagca gccattggag taagtagagt
     9481 tccacaatat cctgctgtta atgctaatgc tccaattata gcaggatttc caccttgaga
     9541 caatacaaaa ggaataccaa ttcctgccgt tatgactgaa aatgcggcaa atgcatttcc
     9601 cattatcatg gtaaatataa ccatacctaa tacataagct ataacaccaa ataatctgct
     9661 atcagcaggg actacaccag atatcatggt tgaaataact tcaccgactc cggcttgagt
     9721 aaataaagct cccaatgcaa ctaaaagttg tggtaacata ctagcaggtc ccatttgttg
     9781 aagtaatctt gagccatctt gaactgatgt agcaggtttt gccttagtta ttataagggc
     9841 tgatattaat gcaattattg aacccacacc tattgcaaat tgagaagaat tatcaaaatt
     9901 agaaaggctc atagctacta ctactgcagc aaatgctaat attaatgatg gtataaatat
     9961 tttattttta agtttgtctg cttgttcttt tccaaaattt tcatctggtt cttcatactt
    10021 tgcaaataca acttgtttag aaagtgttaa taaactagat aatattaata ttattcctat
    10081 tatattggct ggaatcaatc taccaaatat gaatggcagt gatataagta tccaaaataa
    10141 tgatgtacca tatttcttat tgttatttat ggtagtaaaa gacttatatg ctataacaat
    10201 cattaaaaat cccattagtg cgtaaaagat ttctaatgat atgtttataa aattttgcat
    10261 ttttattcct cctaaataaa actatttttt atgacttttg tttaattttt tatcatataa
    10321 ggtaaactgg attgtagcca ctacaagtgc tattactgcc ataatagtac tagcctttac
    10381 aatattaagt ccagttacat tgtatccaaa ttgagtcaga gtagatgcta taagtaatac
    10441 tccactacta cctgcaaata agttttgacc ataaaaattt ccatagtttt ctgatgcagc
    10501 agataatgcc tttatggctt cttcatccga ttcttttagc tcatcactat tacttaaacc
    10561 agcagcttgt gccataggat taacaagtgg tcttacaaat tgaggatgtc cactcattct
    10621 tattgataga gcaccagcta tttgtctagc tatcatgtat atagtaagta cttttcctgt
    10681 tgtaagtctt tttaattttt ctattaaaga aacagctctt tgttttaaac catatctttc
    10741 taatacaccg atcactggca atgttaatat gaataatgat acacctctgt ttgagacaaa
    10801 agaatctcct aaaattgtaa aaatttgatt aaaatctaat cctgcaacaa ttcctgttgc
    10861 cactcctgct aataatacag taaatagtgt gtcaatttta agtataaatc caattacaac
    10921 tatcaatata ccgattagtt ttatcataaa ttgttctcct tccatttcta tatgttttaa
    10981 tttaaattta tttaatggat aaagttaaac tctttttata gtatttgcgt taataggact
    11041 attattataa aatttaataa aaaataggga agatgtttta aagtatcaaa gtaaataatg
    11101 gttgtgtata ggaatttgaa taacaaagat tatattttaa gctaatatga attgattttg
    11161 taagtctata gtttaagtat atgtcaatat ttgaaatagt agttttaaat taaacattat
    11221 attataaaaa gagaatgtgc caaacataga gacaagtgta tttttctctg ttgaaatgat
    11281 aaaaatatat ttgatttcat acaaaaagag agtgtctaat gaatttttta gttcattaga
    11341 cactctcttt ttgttatgat taataaaaca tatttgttat gattaataaa gcatatatta
    11401 agtgctagtt tcattagatt tcactaagaa aaataaaatt ttgataaagt ttaaagacta
    11461 ttttgagcaa tattacttat aaataaaaga atttatcata gaaataatta gctttatttt
    11521 agctattaag atttattaaa aaattatcta tattatcctg tgatattcta aaactgtcat
    11581 aattaccttt tgaaacagcc ttcataataa gtttatctaa aaatttcatt ttttctaatc
    11641 gagcttcact accaaaaatt ccaatagcat ttgcactttc taataaggat gatggaaaat
    11701 ttgattttaa atatttatct gcttgttcag aaaagccaca gcaaaggaaa atgccaactt
    11761 gttttttgtt taataactct acattatcat tacatagcac ctgtattttt tttgaaactg
    11821 aaccaacata tatagaactt ccaataataa ttttatcaaa ttttgataac tcaattttat
    11881 tattattatt attaatatca acaatagtga cattatctga tagtttattt tttagtatat
    11941 ttgcacagtc ttttgtacaa ccatatttac ttgaatatat aataatagta ttcataatta
    12001 ttctcccttt cttaaagcat tttctacagc ttttcgtata gtattactgc tataagttgc
    12061 agaagttata gcatccacat ctaaagactg ttgtttaata atacttgttg taattttttc
    12121 agcaggttta cctaaaagat ggtcgtgttt taagatatta atattttgga ttttaccatt
    12181 attaacttca acagacacag tagcttttac caagttatta tcagcacttc cagtataaat
    12241 gccattttta acattattaa tatctatatc ttttactacc aagttttctg gctttaagtt
    12301 gcttacaatt acaataataa tgcatataaa tacaaagaat agagcaatag atatttttac
    12361 atattttttc tttttattag tagccttgtt gcgaattttc attgtaaagt cctcccacta
    12421 aaatatctaa gaaattttct ataagttgag aaaaattatg taaagaatat agtttatttt
    12481 caacaacaca taaaacgaat cctaacaatg cacttataag catccatgag cttttatcta
    12541 tatttttttt gaatattcct tgttcaattc caatggaaag gaatttatca agcattttta
    12601 taccattatt gctaggcgta ttgtttgtat tttcagatgc aaatataata ttcattctac
    12661 tatgcatgat agattttgcc atctctggtt cactagaaag agtttttata aagctagtca
    12721 taatttctat gatttgttta tctattggta gtgatgagca attattcata atgtcaatgg
    12781 cattactttc tactgtgtta taaagtttat tggtcatatc gctgataatc tctgctttgt
    12841 ctttgtagta aagataaatt gtagttggag aatattctat ttttgtagca atttttctta
    12901 ttgataaatt ttcatatcca tgttggttta taatttcgat gctagcatcc ataatctttt
    12961 tcttcatttc ttctttttca tttttacgtc tttctttgat actcatataa tcacctcttt
    13021 gcttaacggt gttaagttaa tttacactgt tattgtataa tttaatttta atactgtcaa
    13081 taaattatta aataatataa gttaatatat ttttatggat aataaattaa tgttattaat
    13141 tgtaatattt aagatattat tgtataataa taatcggttt aaatgcgcca catcttatta
    13201 aagttggttc ataattctaa ataaacttta ataagataca ttaaaatata ataaattctt
    13261 ttatgctata aaatttattt tatagctatt aaatcagatg tttaaaaatt ttttaacggt
    13321 ctatagtttt taagagtctt atagtaagta gcctaaaaat atgattttac atctgtactt
    13381 gttcgagaac tccaagtata aaaaactaag gaattatata gtggcagtat ataattaata
    13441 cccttagaca cacctttttg aaagtggaaa aaagatgtgt agttctcctc gaccacaaat
    13501 caaggaggat aaaaaatgga aaagagaaaa gtaataattg attgtgaccc aggaattgat
    13561 gattctttgg caattcttct ggctttaaac tcaccagagc tagaagtaat tggaattacc
    13621 acatgttgtg gaaatgtccc agcaaatata ggtgcagaaa atgcactaaa aacacttcaa
    13681 atgtgttctt cactaaatat tccagtatat ataggagaag aagcaccact aaaaagaaaa
    13741 cttgtaacag ctcaagatac acatggagaa gatggtattg gagaaaactt ttatcaaaag
    13801 gttgtaggag ctaaagcaaa aaatggagca gtggatttta taataaatac tttatataat
    13861 catgaaaaag tatcaataat agcacttgca ccacttacaa atatagctaa agcacttatt
    13921 aaagataaga aagcatttga aaatctcgat gagtttgtat ctatgggagg agcatttagg
    13981 attcatggaa attgctctcc agtagcagag tttaattatt gggtagaccc acatggagca
    14041 gattatgttt acaagaattt atctaaaaaa atccacatgg taggtttaga tgtaactaga
    14101 aaaattgtac ttactcctaa tattattgag tttataaata gacttgataa gaagatggca
    14161 aagtatataa ctgaaataac tagattttat attgatttcc attgggaaca ggagggaata
    14221 attggctgtg tgataaatga ccctctagca gtagcatact ttatagatag aagtatatgt
    14281 aaaggatttg aatcatatgt agaagttgta gaagatggaa tagctatggg tcagtctata
    14341 gtggattctt tcaatttcta taaaaaaaat cctaatgcaa ttgttctaaa tgaagttgat
    14401 gagaagaaat ttatgtacat gtttttaaag aggcttttta aaggttatga agacattata
    14461 gactctgtgg aaggagtgat atagtgtgaa aagaaaaata tgtgtaaaaa ctattacact
    14521 tatagctttt ggtatttcta taaatataat aggcgctttt atagctatgg gattaagatt
    14581 acctgtatac ttagattcaa taggaactat aatgatagct tcacttttag gacctaaata
    14641 cgctgttgtt acaggtgtgt ttggaagttt aataagtgga attacgtttg atgtgtattc
    14701 tctctatttt gcaccagttc aaatatcaac cggattactt gctggaatag catttaaaaa
    14761 gaatttttta aggggtttaa aaactccact aggagtactt ttatttgcca ttccaacatc
    14821 tataataagt tcaataattt cagcattttt atttggaggt atgacctctt caggttcatc
    14881 atatatagtg caaatcttga aagtgttagg actgggggat gtttttagtg tgtttgtaac
    14941 ccaagtattt actgattatg gagataagct gttagcagtt gttttagtga atttaggatt
    15001 aaatgcagtt ccaaaaactt taaaggtatc attgactgga ggaaaataga tagaaaatgg
    15061 atagatatag tgagataaca aataaaaatc aaagagaaat tgttctgctt aaaggatttc
    15121 cttgtatatg gggaaagtgt tctttttgtg attatataga tgataattct aatttagaag
    15181 aagagatgaa taaattaaat ctaaaggttt taaaaaacgt aactgggaaa tatggagttc
    15241 ttgaagtcat aaattctgga agctgttttg aattacctaa ggatacttta gaaaaaataa
    15301 agtgtattat aaaagagaag aatataaaaa aattattttt agaaagtcat tggtcttaca
    15361 aaaataggct taaggaaatg agagagtatt ttgaaattcc tgtggtattt aaaattggag
    15421 ttgaaacctt tgacaatgat tttagaaata atattttaaa taaaaatgca aattttaaga
    15481 ctccacaaga tgtaaaagaa tattttgatt caccttgtat tatggttgga ataaaaggac
    15541 aaacaaaaga gatgatagat aaagatatag agattatcct aaatactttt gaaaaagcga
    15601 cagtgaatgt ttttataaat aattcttcta gtataaaaag agatgaagag ttagtgaaat
    15661 ggtttgttag taagtataaa ttcttggatg aaaatccaaa tatagaagtt ttatacaata
    15721 atacagactt tggtgtagga gattagcatt aatttagttt aaatttaaaa ttgaaaagtt
    15781 tcttatgttt ttgaaaaagc ttctaatagt tagaagcttt ttttgtagag ttttatgtta
    15841 caataagaaa aaaggaatta aggagcaatt aaatgatgtt tgatagggga aaatggaata
    15901 aattggatta taatgaactt ttagaatata tgatgtctat acaagatata aaatatagag
    15961 attttaataa aaagcttata cctggaacag aaaatataat aggtattaga gttcctaatt
    16021 taagaaagtt atcaaaagaa ataagccaag ggaattggaa ggaattttta gaagtagcac
    16081 aggatacata ctatgaagaa gttaggctac aaggtatggt aattggcaat ataaatagta
    16141 attttgaaga aactttatac tatgtaaaaa gatttatacc taaaatcgat aattggtcag
    16201 tttgcgatgg attttgttct gatttaaagt ctatgaaaaa atataaagaa aatatgtatg
    16261 atatacttaa aaaatatgtt tattcaaaga atccatggga aattagattt gcattagtta
    16321 tgtttttaat atattatgtt gatgataaac atattaaaga aatatttgaa tactgtaata
    16381 atatacaaag tgaagaatat tatgttaaaa tgggtatggc atggttgcta tcaatttgtt
    16441 ttattaaatg tgaagaggaa acttttctct atataaaaaa taataattta gatgatttca
    16501 cttataataa aatgctacaa aaaataattg aatcaaatag agtagattta gaaaagaaga
    16561 atataataag aagtatgaaa agaaagacaa gaagatgttg acggaggaat attaatgaat
    16621 aacaatgaag acagaattga caaattttta gaacttcaaa gcaaaggatt taaatttgca
    16681 gagatagctc aaaatctaaa gattagtcaa agtactttaa gaactttttt aaataaaaaa
    16741 ggatataagt cagaaaaagg taaatacata ttaaaagacg aattaaatta taataaaagt
    16801 ttggataata gaaacttgga agaagagaaa gctaaggagt atgaaaaaag taaaaagaat
    16861 actataagta atagaaaaaa agtaagcacc aaaaaaaaga tttctaatga aaaggcttta
    16921 agtaaaagtg ttgaacaaat tgagtttaat gatacaggtt taaaaaatgc aaagccaaaa
    16981 caaaatatta agcctaagaa agatagaaaa ataaatataa ctcaagaaga tttagataag
    17041 ctttgtgaag tatatgattg gtatttagaa gtaaaagatt acaatataaa gcctaaaaaa
    17101 gtatctaata aaaaagatgt acatatagat gatgaacaca tagatgaatt aaaaacaaca
    17161 agaatacgta tagataaaga aatatggaat gattttgaaa gattatgtag taattcgaat
    17221 tttgaaaaac atcaaatact tacacaggca ttgaaagaat ttatgaaaaa gtataaaaat
    17281 ctactataaa atactactat atgaggtgct taatatgaat aaaatagtaa aaaaatcaat
    17341 ttctctaatg attatattaa caatatttat ttttatgttg acagcatgtg aaaaagatga
    17401 acagcctgat tcagttgata ttcaaacaga agataaaaat gagataaaaa tagatgaggg
    17461 aaatgcaaaa gtattgaata taggaaaatc agaaattata aatatagatg aaggtgataa
    17521 agtagataca tcaacaaagg tagaaaataa ttcaacattt aatatatcca atgttgaatt
    17581 gatatataat gaatatgatg agaataaaaa aattatatct tcggatagta aggctttgtt
    17641 ggacatgact ttaatgcctg ggaaagttgc ttacatagaa tgtgggcata aaacttttgt
    17701 aaagggtgta gaagtttatg catatgaata tgaggcagag ggtaagattg tgtatgttaa
    17761 tttaaaagaa aatactatta atataagaaa taataatatt aagctagaaa atagtagtca
    17821 gtatgaggtg ctatctacat cagaacttaa aaaagtaaat gaatcaaatg agggaattac
    17881 gtatcaagta aaagtaaaaa attcatcatc aaaagattta ggaaatatta ttcttaagac
    17941 agcagaagtt aatgaaaatg gagaatattt aacagtaaat cgtgtaccat catataagat
    18001 tttaaagcct tcagaagaga cagatataga tatattgtgt tcaacaaagg caaaaagtgt
    18061 tgaaatagtg ggatatacgt atgatgatat aaaagaaaaa gccaatgtgg atattgactt
    18121 aaaatctcat aaagttaaga tagacaaata aaggtagggg atacttatga aggttttaat
    18181 agttgaagat aataagatac ttttagaaag tgtagtagaa gaacttagca agcattttga
    18241 aactgaaaag tgtgaagatg gagaagaagc tttatactta ataaatcaaa atatatatga
    18301 tttagtcatt ttagacttaa tgcttcctaa tataaatgga tttgatatcc taaagaaaat
    18361 gagaattaat aatatagata cacctgtact tatattgaca gcaaaagaaa cattagatga
    18421 caaagtagaa gcatttacta taggagctaa tgattatttg acaaaacctt tttatatgga
    18481 agagttagta gctagggtat acgctatatt aaggactaat ggaaagataa aagaaagaaa
    18541 cggattagaa tttaaatctt tatatttaga tacactagaa aaaagagtgt atattgaaaa
    18601 agaagaaata aaacttcaaa acaaacagtt taacttgctt gagtattttg tattaaataa
    18661 aggttcaatt ttattaaaag agcagattta tgatagaata tggggaattg attcagatgc
    18721 aactattgaa atagtggaag tatatgtaag taatctaaga aaaaagctaa gtaaatatgg
    18781 atatgataaa tatattaaaa ctaaacgtaa ggtaggatat atattcgatg ataaataaga
    18841 atgtatttac cagtactaaa aatcatttaa taaaaatgta tattattgta gtaggtagtt
    18901 ttttaataat cttttctata tttatatact catattttag gggacttaca tatagtggaa
    18961 ttgatagtga gataaatgat gaacttgaat atatagtctc tcaatttaaa agaacttcat
    19021 ttttgaaccc aataagactc aaagatccta aagatatggt gtatgtatat gaagatggaa
    19081 ggataagtta ctatactcaa aatgaatatt ttgatgagtt acttccagat agaagactag
    19141 ataaaaaaaa ttcgtttttt aaatacacag aaaatggata tacttttaga gaattaaatg
    19201 tagatgtagg tagataccaa atacagataa ttagaaatat agattctgag atgaattctt
    19261 taagacagct tacatctgtt cttataattg gaatattaat ttctgtaata attacttact
    19321 ttgtggctgt atatctcact agaaaagctt taattccaat agaaacagca tggaaaaatc
    19381 aagctaagtt tatacaagat gcttctcatg aattaagaac accaataaca atagtttcat
    19441 caaaattaga aagtatgtta aaatccccag aaagtacagt aaatgatgaa gttgaaacaa
    19501 tagctactgc aatgaaggaa actagaagac ttaaaaaaat gataactgat ttattgtctc
    19561 ttactaaaga ggactctatt gttaaagtta atttagaaga aatagattta gaaaagttat
    19621 tggaagaaat atcagaagac tatattgaca ttgctgagtt tcaggaaaaa agattcgtgt
    19681 ttaattctaa actgaagaat aaagttataa taacagataa aaataaatta agacaactta
    19741 tattaatttt cattgataat gcctttaagt atacaaaatt gggtgatgag atagcattag
    19801 aattgaaaga agatatagag gatgaggtaa ctttacttat aagtgatact ggtataggca
    19861 taaaaaaaga agagatacct cttatatttg atagattttt tagaagtgaa aatgtaagaa
    19921 ataaagattt agaaggttct ggaataggac tttcaatagc tcgcatgatt agtttaaact
    19981 tatctattga tataaatgta acttctgatg tagatattgg aactacattt gaattgtcaa
    20041 taccaaaaaa gttaaaataa taaaaatata aaaaattgtt tttagaaatg aagaatgggt
    20101 atactaatta gtataaaata taaaaagacc ttataaaagt aaatttatat ttataaattt
    20161 catagtataa ttatataggg ataataaagt ttaataggag gatttaaaaa tggcaaaagt
    20221 tataaataca tctgaattta gaagtagtgt tgaaggtagt aagggagtag ttgtagttga
    20281 tttctttgca acttggtgtg gaccttgtaa catgttggga cctgtatttg cagaattagg
    20341 tgaagagatg aaggataaag ctagatttgt aaaagttgat atagatgaga gtttagaaat
    20401 agctcaacaa ttcaatgtat ctacagtacc aactatgatt atatttaaag atggaaaacc
    20461 agtagaaact ttaattggat tcatgccaaa gaacaaaatt gaaatgcaag taaaatcata
    20521 tttataaaaa atgggtgagt attatgagat atgatatagc tataatcggc agtggtccag
    20581 ctggattgtc tgctgctata aatgcaaaaa taagaaataa aacaattatc atgtttggta
    20641 atgataattt aagtaataag cttgttaaag ctccttcaat agataattat ttaggttttt
    20701 atgatataag tggtgatgaa ttaaaagaca agtttaaatc acatatagat agtatggata
    20761 tttcaattga aaataaaaga ataaataata tttatgctat gggagaatac tttacaataa
    20821 tgtctggaaa tgatatgtat gaagctacta ctgtaatcct tgcaactggg gtagagtata
    20881 ctagaccaat caagggtgaa gaagaatttc taggaagagg tgtgggctac tgtgctacat
    20941 gtgatgcacc actttataga aataaaaagg tagcagttat aggatataat gaagaatcta
    21001 aagaagaagc aaatttttta agtgaactca cttcaaagac ttattttata cctatgtaca
    21061 aaaaagataa tcttatgaga agttcagata acctagatga ttctattgaa gttatacatg
    21121 acagaccagt acagatagat ggagataaac ttgtaaacaa agtatctttt aaagaaaacc
    21181 acattgaagt tgatggagta ttcgttataa aagatagtac ggctccaagt gcattggttc
    21241 ctggaataga gattgatggc attcatatta aagttgacaa taatatgaag actagtatag
    21301 atggatgctt tgctgctgga gattgtgttg gaaaacccta ttcttatata aaagcagctg
    21361 gtcaagggca aatagcagct ttaaatgctg tttactattt agataaatta aagagggctt
    21421 aatatttata ttgattaact ttttaaagat atttataaaa aactgtcttg aaataatttt
    21481 attatttcaa gacagttttt tatttttaag ttattaataa aagtatttta aaatctatat
    21541 gtaattactt ttattaataa aactcaaagt tatattagta aaaattggaa ttatttataa
    21601 tatactccaa aatttaaaaa taatggttga aaaatctaat taagagtact ataatttaat
    21661 taagtaatta cttaatcaaa atgaggtact tatggaaaac ttggttaaaa tatttaaagc
    21721 attgagtgat gaaactagac ttaacatact tatattagta tccaagagaa atatatgtca
    21781 aaaggggatt tctaaatatc tagggatttc agattctgct gtttcacaac atataaaaat
    21841 attaaaagat gttgggataa taacaggata taaagaagga tactatgttt tatatcatat
    21901 taataaagaa tcatttaata tatgtgtaga ttttataaat tctatgttaa gtaattctag
    21961 tgaaagcttt atagatgtat ttgatgtaaa tacaatacat ctaggatgca gtaaagaatg
    22021 taaatctata aaaaaatgtt gtaagagaag ggagaaataa taatgaaagt atgtatacca
    22081 gtagaggaga ataaaggatt ggatagtaaa ccatatggac attttggttc tgctccaatt
    22141 tttgttgtgt gtgatttaga aagtggagaa gttaaatctt tagataatgg agatttagat
    22201 catgaacatg gaaaatgtca accattaaaa gcattgtcag ggacagctgt agatgcagta
    22261 gttgttggag gaataggaca aggagctata attaaattaa atggaatggg aataaaagtt
    22321 tacagagcag aaggtgatac tataagtgct aatttagact tacttaaaaa tggaaaatta
    22381 gtagaatttc catcagacca tacttgctca catgacggat gtggacatca ttaaaaaaaa
    22441 acagctgatg aaatatatac ttcatcagct attttttctt ttaataaata cgattttatt
    22501 cttttaaaac caaaatttag atttttatgt tttttgtagt atccttaata atatttcaag
    22561 tttacaagtt tactactttt agattttttt tgtatgtttt tctatatagt caatatcatt
    22621 taatatgtca ttgagttctt tattaagtgt ttttaaatca tcaccatcta cattgtagtt
    22681 taaaaatagt tcctctacat tatcaatgat agaattgact tttctagtaa cttcaacaaa
    22741 tgtcttaaag ttactactat ttttagcatt tagaaatttt tttaaatcct ttgatataaa
    22801 tgatactatt tcatattcat cattaaatag acctctacca ataggatatg gttcaattct
    22861 ctgtaaaaaa tattggtctt tagcattaat attataagta taagatattt cccaaccgtc
    22921 tcctaaatca aacttgataa aatataatgt actacttact tttgatattc ttgtggcacc
    22981 aagctgtctt aaaagaactt caatcgaatc accttgaaca gtattaatat tcatatatac
    23041 acatcccctt tagtattatt atactatatt ttttaaaatt ttataattaa ataaacagta
    23101 gatataataa tagatataat cataagtgga aaacctattt tgaaatactc cttaaaagag
    23161 ataggatgac cgtgtttatt acctatacct gctagtacaa cattagcaga tgctcctatt
    23221 agtgttccat ttcctccaag acaagctcca agagatgttg cccaccatag tggcattaca
    23281 tcaatacctt cagcttgcat agtcaaaata agaggaatta gtgttgcaac aaatggaata
    23341 ttatctaaga aagaagagac tatagctgac aaccataata tcaacaacat tgtaaataca
    23401 agatgtcctt ctgtaagtcc aatcaatgct tgagccaatt tgtttattat cccaacttct
    23461 actaatccac caactactac aaataatccc ataaagaata aaattgtaga ccattcaata
    23521 cctgccataa tttcatctac atcttgtttt cctattaaaa gcattataga tgctcctgtc
    23581 agtgctacaa cagaagaatc tatgtggatt gtactgtgaa acataaaacc aaaaagtatg
    23641 aatgctatta atataaggct ttttataagt agaggtttat ctttaacaga ttttttttca
    23701 tctagtttta atattgcatt tttagctctt tcattcacaa caagttcttt tccataaata
    23761 aatctaaagc atattatagt tatagcaagt attactataa cagcaggacc taaatttatt
    23821 acaaaatcca taaaactaag attggcagca ctaccaatca taatatttgg agggtctcct
    23881 ataagagtag ctgttcctcc tatatttgaa gctaaaatct gtgttattaa aaatggaact
    23941 ggattaagtc ctaatatttg tgtaataact atagtcattg gtccaataag taagacggtt
    24001 gtaacattat ctaatacagc tgataaaata gctgttataa tagcaaaaca aatcattatc
    24061 ttccatgggt cacctttggc tttctttgca gtccagatag ctatatattc aaataacccc
    24121 gagtttttga caacagctac aaatagcatc ataccaacta atacaccaat agtattgaaa
    24181 tctatatggc ttaatccttc atcaagtgta agtatattga atattatcat taatagtgca
    24241 ccaccaacag ctgctatgga tctgtttacc ttttctgtaa taatagctgc cataactatt
    24301 aaaaatatac atatagctat tatttgttga aattccataa tataagtacc tccatttgta
    24361 ttattttgtg cctaatgcta gtactatata tagtattact tttttattgt tttttcaatg
    24421 gatgtatgaa agtatattat ttaacaaagt ttttggaaaa ttaacagaat ttaaagaaaa
    24481 tttttgttat ttaaataaat tttaaaatta ttccattgat aataatatta aacgacaaac
    24541 gaaaaaatat accctttaaa ctagaattta ttgtatctaa taacaattat aaaatattaa
    24601 atatatctat attataaaaa caataaaaaa aggtttgatt tgaatataaa actgcaatat
    24661 attaaaaaaa ttattcattt taaaccttgt aatttgattt tataaatatt ataattaaat
    24721 taatgaattt ttaacaagga attattcatt tttttgattt taagatttat tgagaaaaca
    24781 aaactaaata ggggggtaat taatataatg gattttttca atggtatttt aacgagtatt
    24841 aacaatgcag tagtatatgt aaacaattac ttatggtcgt atatcttaat tataatgtta
    24901 gttgtaattg gtatttactt tactattaaa acaaattttg tacaattcag atattttata
    24961 gaaatgttta gacttctggg tgatggtaca gcgaataaaa atgcaaaaaa ggaaggaaaa
    25021 atctcttcat ttcaagcatt ttgtataagt acatcttcaa gagttggaac tggaaatata
    25081 gctggaatag caattgcagt ggtagcagga ggacctggtg ctgtattttg gatgtggctt
    25141 atagcattaa ttggttctgc atcaagcttt gttgagagta cattagctca gatttacaaa
    25201 gtgaaagatg gtcaagcttt tagaggtgga ccagcgtatt atatggaaca aggtctaaat
    25261 aaaaaatgga tgggtatttt attctcaata cttataacta tatgttatgg ttttgtattt
    25321 aatgcagttc aagcaaatac tgtttcactt gcatttaata atgcatttgg aataagtaaa
    25381 atggctatgg gaataggtct tggaatttta actgcaatag ttatttttgg tggagtgcat
    25441 agagttgcaa aagtttctga aataatagtt cctgtatttg caggattata tatattagtt
    25501 gctttggcta tagttgtaat gaatataaca gagataccaa gtgttatagc attgatagtt
    25561 gaaagtgcat ttgattttaa aggaatggcg attggtacat ttatgggagt tgtaatgact
    25621 ggagtgaaga gaggtctatt ttctaatgaa gcaggtatgg gtagtgctcc taatgctgct
    25681 gcaacagcac atgtaactca tcctgtaaaa caaggtttaa ttcaatcttt aggtgttttt
    25741 acagatacta taataatatg tagttgtaca gcattcatgg tattattata ttcaggttat
    25801 agtagtagtg gagcaactgg aattgaatta gctcaagaag ctttaactca tcatatagga
    25861 ccagttggaa atatatttat agcagtttgt atattcttgt ttgcatttag ttctatagtt
    25921 ggtaattact attatggtga atcaaatatg gagtttatga gtggaagtaa gactaaactt
    25981 aatgtattta gagtttttgt tgtaggtatg gttttatttg gttcacttac tcaagtggat
    26041 gtagtttgga atcttgcaga cttatttatg gcattgatgg cgataataaa cttaatagca
    26101 atagcacttt taggaaaata tgcatttatt gctttaaaag attatacaag tcaaaaaaaa
    26161 tcaggaataa aagaccctgt gtttattgca gatgaaatag aaggcctaga aaaggtatca
    26221 gagtggcata gggaattatc tgaggaacat ttaggataaa aaatagtata aaattataaa
    26281 accattttga aagactagag tatgtatata ctttatatga atataatatt gaattaattt
    26341 atattactgg aatttttaat tccagtaata tttttttatt atataattta gctttattaa
    26401 aagtttttat tattaaaaat agcttattta tcgtaaagtg aatgatattg ggttttaatt
    26461 attatgaatg ttagtattta atttaaagac ttaaataaat ttgccatttc tagcgcattc
    26521 atagcacaat cataaccttt atttccagct tttgtacctg ctctttcaat agcttgttca
    26581 atattttctg tagttacgat tccaaatata acaggtaatt cactgtctaa agatactttg
    26641 gctatccctt tagagacttc actacataca taatcataat gagaagtagc ccctcttata
    26701 acacagccta aacagattat agcatcatac ttaccacttt tagccatttt ttgagcaaca
    26761 agaggaatct caaaagctcc aggtacccaa actatttcta tattttctgg agaaacgtca
    26821 tgtcttaaaa gacagtcttc agctcctgat aaaagttttg atgttataaa ttcattaaaa
    26881 cgagaattta ttatacctat ttttaaatct tttccaataa gttttccttc atatatcatt
    26941 tttacttcct ccaattttat ttatgttata gatttatatt tataaatatc tttaatttaa
    27001 ttataaaaaa ctacttagta gaaaaatatc atcatataat catttatata atatctaaaa
    27061 gatgtcccat tttatctttt ttagttttta aatatagctt attaaagtca ttaatttctt
    27121 cattaatagg gattctattt tcgattttta ttccatattt ctctaattga tttattttat
    27181 ctgggttatt acttaaaaga tttatacttt ttatgtttaa attttttaat atttgagcag
    27241 ccatatagaa atctctcata tcttcttcaa atccaagcat taaatttgct tcaacagtat
    27301 cgtaaccttc ttcttgaagt ttataagcct taattttatt taaaagacca atacctctac
    27361 cttcttgctt catataaatt aaaactccac ttccattttc cacaattttc ttcatagaag
    27421 attctagctg taatccacaa tcacatttta gagagtggaa cacatctcct gttaaacatt
    27481 cagaatgaag tctaactaga gtattttcta tatttatatt tccataagta agggcaatgt
    27541 gttcttcatt tgaataagta tctctatatc ctatgatttc aaaatttcca tattttgtcg
    27601 gaaggaaagc actagatact ttttctatag tagtttcatt tattttgcgg tattctataa
    27661 ggtcttttat agtgatgatt tttaaattat gcttttcttt gaaaatcatt aaatcatcaa
    27721 ctcttgccat tgtaccatca tctttcatta tttcacagat aagaccaatc tctttaaaac
    27781 ctgcaagtct agcaaggtct actgaggctt ctgtatgtcc atccctgaca agaaccccac
    27841 cagtttttgc tattaaagga aagatgtgac caggcgtttt gaaatcttta cttgtagaat
    27901 tttcatcaca gaataatttt acagtttcag ctctttcata tgcagatata ccagttagag
    27961 tatctgctcc atctatagta acagtaaagg ctgtttgaaa tgtatctgta ttttctctaa
    28021 ccataagagg tatattaaga cttttaaatt ttttttcagt ggcaggtaga caaattagac
    28081 ctctaccata tgtagccata aaatttattg attcaggagt agccatttca gctgccatca
    28141 ataaatctcc ttcattctct ctactttcat catcaaccac gattaccatt ttcccatttt
    28201 ttatatcttc aatagcttct tcaatagtat taaacatgta attcactcct ttattatttt
    28261 taaaaaccat ttttaaataa aaaatcttta tctatatttg atttatggtt attactatta
    28321 gaatttaatt ccttgtagtt attagccata aaattattaa catactttcc aattacatcg
    28381 ctttctaaat taacgaaatc tccaacattc tttgtcaaga gaatagtgtt aattttagta
    28441 tgaggtataa tagaaacctt aaatatttca tcatctaaat atgatatagt taaacttaca
    28501 ccatcaatag caacagaacc tttgagtatc attgaggata aaagtttttt atcaggtcgt
    28561 atacttacta aaactgcatt ttcatctttt tcaaatttgc agatagttcc tttaccatca
    28621 acatgacctg ttactatatg accaccaaat cttgaagaaa gactcatagc tctttcaaga
    28681 tttaccttgt catttgcatt taataaacct aaatttgtgg aacgaattgt ctccatcata
    28741 acatcggctg taaattcatt ttttccgagg tttgaaacag ttagacaaac tccatttacg
    28801 gctatactat ctccaagatt tgtgccatct aatattttat ttgatagtat ggttactttt
    28861 ccactcttac cattagcagt aatttttcta agtattccaa cttcttctac tatgccagta
    28921 aacatcctaa cctcctatat gaatgtatta acaaaaattt aatttaaaac atctgattct
    28981 ataagaatat cgccatctat caaagttaca ttattgattg ttaactgtgt tgaatctgac
    29041 aaatgattta ttccttctcc agaaacgaat gtaggtgcat ctttaccacc tataatcttg
    29101 ggtgatagaa ataatttgac cttatcaact aacttatttc taaagagact gtcattgaga
    29161 gtagcaccac cttcaactag aacagaatca atgtttaact ctcctaattt atacattaca
    29221 aactccaaat ctactaagtt gttttttgaa ggagaaatta ttgtttcaac attattttct
    29281 tttaacatag ataattttat actttctttg caacatgtga aaactatagt tttactggtt
    29341 ttatctttaa ccaaattact attaagtggc aactttaggt gtgtatctat tactattctt
    29401 gtaggatgag acactttttc ttgattgagc ctacaagtta gtaaaggatt atcgttcaaa
    29461 actgtattta tgccaaccat tatactttgg tatttatttc tgtatttatg agttagatac
    29521 cttgatgatt cattagatat ccattttgat tctagtgatt ttgtagcaat tttaccatct
    29581 agacttactg ctgattttac aatacaaaga ggtcttttgt tttttatatg ataaaaaaat
    29641 gcctcattta aatctcttgc ttcatcttct aatattccca cagtaacatc aatattgtta
    29701 tcttttaatt tttttactcc attaccacaa acaagtggat ttgggtctaa tgtagatatt
    29761 acaactctct taatcttatt ttgtataatt ttatctacac aaggaggcgt tttcccataa
    29821 tgagaacatg gttctaagtt tacataaaga gtagagtctt ttagactttg tttagcactg
    29881 tttatagcat ttacttcagc atgattagag ccaaatttct catgatatcc tttgcctatt
    29941 atattactag ctttaacaat tacacaccca actaatggat ttggatttac aaagccttct
    30001 ccatttttag ccaattcaat agcttttttc atataataaa tatctttttc tttttgattc
    30061 acaaaattca caatatgtac acctcctgtt taattggata atttttaaac aaaaaaccct
    30121 gagaattaac tcagggttta gaataatcta caataaatgt ttaataaatg tagaacaaaa
    30181 ttgctctaca aaaacactca ttaaatatct tcttccatcc agactttact gtcggctttg
    30241 gaatttaacc aaatcaacca agttttacct tggctcgcgg gctataccgc cgattgggaa
    30301 tttcacccta ccccgaagat taagtattaa atttatattt agaatgtatc ataaattatt
    30361 ttactttgca aactattttt ttaaaaaata tatgttaata tataatatag aatgtctata
    30421 aactttgaaa taccacgatt gtgaatataa aaataaaaat ttgaacataa tcaggaaaga
    30481 agtctaatta atttaaatta ggagagtaat atatgaataa aaaactttta gaaatactat
    30541 caaaatacta tggatataca agttttagaa aaggtcaaga atctataata aactctatct
    30601 taagcgaaaa agatgtgtta gctattatgc caacaggagg gggaaaatca atatgttatc
    30661 aaattccagc tttgatttta gatggaatga caatagtaat ttctccactt atatcactta
    30721 tgaaagacca agtagatgcc ttaaagacta tgggaattag tgctgctttt ataaatagtt
    30781 cattaagtag caaagaattt aatgaaatat taaataatat tagaaaaaat aattataaaa
    30841 tactctatat tgctccagag agattagatg cacaagagtt tattgagcta ataaataata
    30901 acaatgtaag tcaagttgcg atagatgaag cacattgtgt atctcaatgg gggcatgatt
    30961 ttaggttgag ttatagaagg atttctgatt ttataaaaaa tttgcctaaa agacctatag
    31021 ttacagcatt tactgcaaca gcttcagaag aggtaagaac tgatataata aatttactgt
    31081 gtttagaaaa tccagattat tatataactg gatttgatag agagaattta tcaataaata
    31141 tagtaaaatc atctagtaaa aataagtata ttttagatta tattcaaaat cataaaaatg
    31201 agtctggaat tatatatgtt gcaactagaa aggaagttga aaatatatat aatggtttat
    31261 ctaagagaaa tatatctgtg tcaaaatatc atgcaggttt atctaaaaat gaaagaagta
    31321 aaaatcaaga agattttata aatgataatg tggatataat ggtagcaaca aatgcatttg
    31381 gaatgggtat tgataaacct aatataagat gggttataca ttacaatatg cctcaaagta
    31441 tagaaaacta ctatcaagaa ataggtagag ctggtaggga tggagaaaaa agtgagtgtg
    31501 tacttttatt ttctccagga gatgtacata tacaaaaata tttaattgat attggagttg
    31561 aaaatcctga aagaaaatta tttcagcata agaagcttca atacatgatt gacttagttt
    31621 acagtaattc atgctataga aagacaatat tgagttattt tggagaaaat tatatagaca
    31681 attgcaataa ttgtagcaat tgtttagtag agggtgaaat agtagataaa actatagatg
    31741 cacaaaaagt catttcatgt gtagctagaa tgaaaagaag ttatggtgtt actactattg
    31801 tagatgtgct tagaggttct aagaataaaa agattttaca gcttggatta aatactttgt
    31861 ctacatataa cattatgaga gattattcta gtgaagactt aaagaatttt gtaaatacat
    31921 tggtttctca tggtttttta gatttagttg agactctagg caatggaaat agaggtagtt
    31981 ttcctactat aagattaaat gagatgtcta tgaaagtttt aaaaggtgaa gtgaaagtag
    32041 agtttaaaga aattgttatg acaaaatcat tggaagtaga ggatgagtta tataatgctt
    32101 taagagaact tagacattca attgcaagtg aagaaaggat agctccatat atggtatttg
    32161 gagatggtac acttagagcc atgtctagta gttatccaat taataaagaa gaaatgctag
    32221 atatctctgg agtaggagaa attaaatatc aaaaatatgg tagagatttt gaagcagtaa
    32281 ttaaagaata tgtcgaaaaa aataatataa ataaaaaaat agatgaaaaa aatgtcgatt
    32341 caaagaacgt taatagtgat ttcttagagg ttaatacaga taaagtttta tatgaaaaac
    32401 tgttagtcat tagagaagaa tatgcaacaa aggaaaaatt acctgcatat atgattttag
    32461 ccaataaggc tttgaaagaa ataagtggaa gatatccact tgatgaagaa caattaaaag
    32521 atatttctgg cataggtgga gtaaaatttg aaaaatatgg agctagtatt ttggcagagg
    32581 ttaataaata tgtaaaagaa aataatatta atgttatttg ggagaaaaag ggcaaaagga
    32641 agttaatatt agatggagaa agtagaaaaa ataatgaaat agcattagat ttgctaaatc
    32701 aaggaaaaga tatacaaagt atcagtcaag aaatagaagt ttcactgtca accattattg
    32761 gatatgtgca tgactatgta aaggatggac atagtatatc atttgaatta ggactagaaa
    32821 atttttataa tgaagataaa aagaagatga ttttagaagt ttgcgaaaaa gttggctatg
    32881 ataatttaaa taacataaaa agaaaacttc cagattcaat aaagtatgaa aatataagag
    32941 ctgtgatact tgactcttat cttgaaaact taaaaaataa tattatagca gagtaatttt
    33001 taacttaata aaaaataaaa tttgtattat tgtgttgaac taataatttt gctgtgttat
    33061 actgaatttg taaaaataat gaaaaattag agctaggggc gctagaattg gctagctgag
    33121 agataaagcc gtaacttaca aaccctaata acctgatcaa gataatgctt gcgaagggaa
    33181 gctttttaat ataattttat tatataaata ggcaaattat tcaatgttag gagaaatttg
    33241 tctaagtgta tatacgatta taagagtgct ttccaaacga ggaaaagcac tttttttatt
    33301 ttcaaaaact ataactttaa tcttggagga ttgtaatatg aactatacaa cacaaatgga
    33361 tgctgctaga aaaggcataa taactaaaga aatggaaatc gtttctcaaa aagaacaggt
    33421 agatgtaaat gaattaagag aattaatagc aaatggacaa gttgttatac cagctaataa
    33481 aaatcataaa tctttaagtg cagaaggtgt aggtaaaaat ttaagaacaa aaataaatgt
    33541 aaatctagga atttcaagag attgtaagga tatagaaaaa gaacttgaaa aagttagagt
    33601 agcaatagac atgaaagctg aagctataat ggatttaagt aattatggca aaactagaga
    33661 atttagagaa aaggttgttg aaatgtcacc tgcaatgata ggttcagtac ctatgtatga
    33721 tgcagtagga tatttggaaa aagaattaaa agatataaca gaagaagagt ttttaaatgt
    33781 aataagacaa catgctattg atggagtaga ctttattact atacatgcag gtcttacaag
    33841 aagtgtttgc caaaaaataa aaaatcatga gagattgact catatagtat ctcgtggagg
    33901 ttctttattg tttgcatgga tggaattaaa taataaggaa aatcctattt atactaattt
    33961 tgataaaata ttggatatat gtgaagaata tgatgttaca ttgagtttag gagatgcttg
    34021 tagaccaggt tgtataaaag attctactga tggtgttcaa attcaagaac ttgtagtatt
    34081 aggtgaactt actaaaagag catgggaaag aaatgtacaa gtaatgatag aaggtcctgg
    34141 acacatggct attgatgaaa tagaagcaaa tgtagtatta gaaaagagat tatgccatgg
    34201 agcacctttt tatgttcttg gaccattggt tacagatata gcaccaggtt atgaccatat
    34261 aacaagtgca ataggaggag ctttagcatg tgccaaagga gtagattttt tatgttatgt
    34321 aactccagca gaacatttaa gattgcctaa tttagatgat atgaaagagg gtataatagc
    34381 tgcaaagata gctgctcatg caggagatat agctaaaaat gttaaaggtg ctcgtgaatg
    34441 ggataataag atgagtaaag ctagagctga tttagactgg tgtgagatgt ttagacttgc
    34501 aatagaccct gaaaaagcta agagatacag agatgaatca actcctactc atgaggatag
    34561 ttgtactatg tgtggaaaaa tgtgctcaat gagaacagtt aagaaaatat taaacaatga
    34621 ggaacttaat ctaatataat ttttggctag tatgactgga ataaaaataa gctgaaaaga
    34681 ggaggtgatt tagtgaaggt taatgggaag gaaattgaat ttgaaaaaga tttgactgtt
    34741 atagatttat taaataaata taatttaaaa agtgatagag ttgtagttga agtgaattta
    34801 gagataattg aagaatctaa ctacaataca tatgtattaa aagacgagga cattgtagag
    34861 cttattagct ttattggagg tggttaaaat aaaaatattt gtaaatgaat tattaacaga
    34921 tgttgaggaa aatacaactg cttataaagt tagagatagc tataacaata aatgtgatgt
    34981 attggtgtta aatggatatc caattaaaac agatatgacc ctttgtgaaa atgataaatt
    35041 aacacttata caaaaaggag ttaagccatc acttgatgaa ttggaaagct tgttgattgc
    35101 tagacatact ccaaatatac ataataaatt aaaaaaaggg aaagttgcta tattgggatt
    35161 ggggggactt ggttccaata tagctatctc tttagctaga ataggagtgg gagaactttt
    35221 acttatagat tatgatgttg tagaaccatc taatttaaat agacagcagt attttataga
    35281 tgatatagga aaactaaaaa ctgatgccat gatagaaaat ataaaaaaaa taaatccatt
    35341 tataaaatta aataaacgag atattttttt aaacaagaac aatatggata ctattaagga
    35401 ttcagattta ataattgagg cctttgatga tgcttcatgt aaagcacagg tatgtaatta
    35461 tgtattaata aatttaaaag ataaatattt aatagcatca tctggtatgg caggttacta
    35521 tgattcaaat ataataacta ctaagaagat aaaagacagg ttttatatat gtggggattt
    35581 tgtgaatgaa gctaaatttg gtgaagggct tatggctcca agggttgcca tctgcgcaaa
    35641 tcatatggca aatttggcaa cgcaaatact tatagatatg taaatattaa aattagatat
    35701 aaaaaagatt gaatattaaa aaaatttaac gtggagggaa ataatatgga taaattggta
    35761 cttggtggac atgaatttaa tagcagatta ttggttggaa caggtaaata tggttcaaat
    35821 aatatattgc ctgaggtaat aaaagaaagt ggaagtgaaa taataacaat ggcacttaga
    35881 agagtggatt tagacaataa acaagaaaac atattaacgt atattccaaa agagatgaca
    35941 atacttccaa atacatcagg agctactaat gcagaagaag ctgtaagaat agctagaata
    36001 tctagaaaaa tgggatgtgg agattttata aaaatagaag taatatcaga tactagatat
    36061 ttacttcctg ataatgaaga aactataaaa gctacaaaga tacttgctga tgaggggttt
    36121 atagttcttc catatatgac accagacctt tatgctggaa gaagacttat agaagctaat
    36181 gcagcggcag ttatgccact tggagctcca attggttcaa atagaggtct tcaaatgaaa
    36241 gaaatgataa gaataatgat agatgagttg gatataccaa taatagtaga tgcaggtatt
    36301 ggaaaaccat ctcaagctat ggaggctatg gaaatgggag ctgatgcagt acttgtcaac
    36361 actgctatag cttcagcagg agaccctgta caaatggcta gagcatttaa attagctgta
    36421 gaaggtggaa gagaagcata tattgcaaaa acaggaaatg tgtcagaatt tgcaaatgca
    36481 tcttctcctt taacaggttt tttaggcaat ttatagaaag tggtgattgg tgatgagttt
    36541 ttatgatgta atagaaaaat atagggattt tgattttgat ggatatttaa ataatgttac
    36601 tgataatgat gttttaagaa gtttatcaaa agacaagctt gaagattttg atatattaaa
    36661 tttgctttct aaaactgcag taaaacattt agaagatatg gcacaaaaag cacataaatt
    36721 aagtgttcaa tactttggta aaacagtatg tttatataca ccaatgtata tagcaaatta
    36781 ttgtgttaat caatgtgttt attgtagcta taatataaag agtggaataa aaagaaaaaa
    36841 attaactatg gatgaaatta gagaagaggg agaagtaatt tcaaaagaag gtttcaaaca
    36901 tctacttgtt ttgactggag aaagtagttt tcactcaagt gtagaatata taggtgaggc
    36961 tatagaaata ttaagagaga aatttccctc tataggtata gaagtttatc caatggaagt
    37021 agaagaatat aagtacattg tagataaagg tgttgaagga ttaacagtat atcaagaaac
    37081 ttatgacgag gaaatatata agagagtaca tataaaaggc ccaaaatcaa attataaatt
    37141 tagactagat gctcctgaaa gaggggcaaa agcaggtatg aggactttat caataggtgc
    37201 acttttaggg ctaaatgatt ttagaaaaga aacttttttt acaatattgc atgggaaata
    37261 cttaaaaacg aaatatcctc atattgagct ttcatattca acaccaagaa tgagaccttt
    37321 taaagggtgt tttgaggaat tggtagatat aagtgatact gatttagttc aagctatggt
    37381 atgtatgaga ttatttgacc ctcatgcagc tataaatata tccactagag aaaatttaga
    37441 aatgagaagt catataatac ctttaggagt aactaaattg agtgcaggtg tatctactga
    37501 tgtaggaggt cattctcaag atgaacatga tactgctcaa tttaagataa atgatgaaag
    37561 tactgttaaa gatgtggaga aaatgctaaa ctcaattggt tatcagcatg tgtttaaaga
    37621 ttgggaaaga ttttaaatgt atctgataac aaacagaaaa ttatgtagtg aggaacgata
    37681 tttagaagtt attaaagagt ctatattgag tggtgtagaa aatataataa taagagaaaa
    37741 ggatttagaa tatcaagagc taagaaaatt atatatgaaa ataaaaacta aaatcaattg
    37801 tatagatttt caagagcaaa tatcagatga aagtttgaaa actaatataa atcaaaaaga
    37861 gtttagaaat aaattcaagg ttaattttat aataaatagt aacattgaat tttttgaaaa
    37921 agtggattgt caaggtatcc atcttccttt taaattattc ttgaatttaa ttgaaaataa
    37981 gtataatttt aatgaaaata aaatactagg attatcttta cataaagtag aggaagtaga
    38041 ttatttagaa aaattaataa gaaatcaaaa tataaaaata gattacataa cattatcaca
    38101 tatatatgaa actaaatgta aagaaggatt aaatccaaaa ggaattgagc ttttaaaaga
    38161 agctaaaaag attactgata ttaaaataat tgcacttggg ggaattttac catctaatgt
    38221 caaggaaaca ttaaaatact gtgatgattt tgctataatg tcaactataa tgaggtctaa
    38281 agatattaaa aaaaccatat caaattataa tgaaaagttg aattagtaaa tatagtatat
    38341 tgaattttta acttaaataa aaataaaaaa gttatctttt aaattttaaa aggtagcttt
    38401 tttattttat atctttgcta ataaaaaatt gtaaaaaggg aaaactttag tcagagagat
    38461 taaacaatat cctagaaaga tttaaaattt agtaaagtat ggaggacatt ataatgactg
    38521 acagtaaaaa gaaacgattt attataccac acacttttac aataatattt ttactaatag
    38581 tcttgatggc aatattgact tggatagtac caagtggtga atttagtaga gaagatgtag
    38641 aaggtagaat ggtagttgta ccagggactt atcaaagtgt agaaagtaat cctcaaggat
    38701 ttgcagatgt ttttaaagca ccaatagaag gctttatcga ttcagctgaa gttgtaggtt
    38761 ttgttttaat agttggaggt gcttttggta ttgtaaatag gactggagct atagaagcag
    38821 gaatagcttc ggttatctct aagtttaagg gaaaagaact tcttataatt ccaatatcaa
    38881 tgattttatt tggacttgga ggtacaacat ttgggatggc agaagagaca ttaccatttt
    38941 atatgatatt tgttccactc atgacatcct tgggatatga ttctctaaca gcaatagcaa
    39001 ttgtatttat aggagcagca gcaggagcag ccgcttcaac aataaatcca ttttcagttg
    39061 gtattgcaca ggcactagct caacttgctc ctggttcagg agtaacttac agagtagtta
    39121 tttttatagc atatatacta ataagtatag catttgttat gtactatgct aagaaggtaa
    39181 aggaaaatcc tgaaagttca atagtatatg aaatagataa aaataataaa tcaatactta
    39241 atcaaacttc aacagaaata aaagaattta cttttaaaga agtatctgtt cttgctatat
    39301 ttggaatagg gatggcaata atgatttggg gtgttttaag tcaaggatgg tacattccag
    39361 aaatatcaat gtgttttgct gcaataggtg tgctttctgg attggttggt agattatctc
    39421 aaattgaaat ctcagaaagt tttatagatg gagcaaaaga tttagcatct gcagctcttg
    39481 ctatagcttt ggctcgtgga atcgtaataa ttgctcaaaa tggatttata atagatacta
    39541 tattaaattc cgcagcagcc tttctaggtg ggcttccaaa ggttatattt gtatatttat
    39601 catttttctt ggaagctgca ttggcatttt taattccatc atcttcaggt cttgcttcac
    39661 ttacagtacc tgtacttgct cctcttggtg atttggttaa tatatcggct caagtgatag
    39721 taaccgctta tcaatttggt gtgggtctta caaactttat aactccaact tcaggagtac
    39781 tgatgggagc acttgcagta gcccatattc cattttcaaa atgggtaaga tttataatgc
    39841 ctatcttgtt gattttgaca gctgtatctc tagtcttttt aacaataggt atttatatag
    39901 ggttttaatt aagtgtagtt ttatttaaga tatttagtaa tggtataaaa tgataatatg
    39961 ttttcaataa attgccacta atttaattag tggtaatttg ttttttgtat ttaaaaataa
    40021 atgtatttac ttgtattttg aattaatatc tacttagaca ttgttttaat tgttatattt
    40081 ttatttaatt aagcattgat ttatcatttt ttatatttca tataacaatg caaaaaataa
    40141 tttttatata gtaaatgaca aaagtttaca ataaatgtat attttcttac tcattcctta
    40201 aaattactca tttagtttaa taactctcat ttcatatgta aagttggtaa aaaaattaaa
    40261 attaaaagta aaaaactaaa taaattaatg ttaatttgaa atatatatga aggtttttgg
    40321 agaatactta cttatgatag gaaaaaatgt taaaataata gtgtaatcaa atataaagga
    40381 ctgaaataat taacagttat taaatattag gagtgtaaaa atttatggga ttaaaagaat
    40441 ttgattttat tgaagagtct attgatatgt tgaggacaat gtcaccaaca ttagaaacta
    40501 tatcagatga aatagaagaa tattttgaaa atatcttgga tgaaaagaat caagaatata
    40561 taaatgtaac ctctagaata aaatcagaat caagtttaag agaaaagata attagaaata
    40621 gatatttaaa aaaatatgga gaagcaagta atcttattca taatgtttct gacttaatag
    40681 gcctaagaat tgagtgtaga tttattgagg atgaaaacaa aatatataga cttttgagaa
    40741 gatattttaa taaaacagat gacaaaataa actattataa taaagagaat aagaatatta
    40801 aattaaaact atctgagagg caacccaata agcaaaaaaa tggatttgaa atatataaaa
    40861 tagatggagt ttttgcgtat ttagatagag aagtaaaatt tgaacttcaa attaaatctc
    40921 ttgtaaatgt gttttggagt gaaatagaac ataaaataat ttataaaaac aatacatatt
    40981 tacttgctga taaatttata aaggatatga tggactctat aaagaataat cttactatga
    41041 tagataatca gcttttaagt atttataaaa actttcattc agggaagtct tttaatatga
    41101 aagtaagtaa aaaagaaatt gaaaaattat ttgcgaaact agtctatgac gctttttctg
    41161 aaaagatgaa taaaagtata ggttttgttg tagattttaa aaaaccatgt gagactatat
    41221 tgaactattc ttttaataaa caagatataa gtgatgaagt tttaggtaat tttatgcttg
    41281 atgagtttgc aagattaaat gaaattgtaa acaaggatat agattttaat gagcaaatag
    41341 aatttgaaag agaaccaaca tttgatgata agttttgtaa agacctagga aatcatttta
    41401 gaagtagatt aaacacagaa tttccatgga acctattttt tagaatatta tttgaaatag
    41461 agccatataa caataccaaa gactttgaaa actttgtaga gtttataaaa gaaaatgtgc
    41521 tactagaaga aaatagggag caattattat gccaatttga agaggatagt aagcttataa
    41581 ttgaagatat atatgattgt atatttaata gtataagtga gattgatagt gttgaaattt
    41641 tatataacta taatcttgaa aaaataaact ttacatcaag tgaaatcata aattgtatat
    41701 gtagagaata tgagtgttat ggtgaatact tagaagaaaa agaaaagctt atgaatacat
    41761 ttaaagaaaa gattattcaa atctttgagt aataggtcaa tgctgaagtg gcgctaatgc
    41821 gccatttttc aaatatatat gaattaagta aaattatttt ggaggaggcg atttgtatag
    41881 agaaaacagg tgtaattttg gcagggggaa aaaattctag aatgggtaga gataaagcct
    41941 ttttagagtt acatgataag ctttttattg aaattgcaat agaagcattt aaaaactttg
    42001 atgagttgat aataatatct aacaatgaag aattatattc taaatacgat attaaagttt
    42061 ataatgatat agtaaaagat gttggtccaa ttggaggaat atatactgct cttgaatatg
    42121 caaagtatga tatagttaca attgcatgtg atatgccata tctaaataat caaatagtcg
    42181 aaagaatagc taataaaatg gatgataaaa gtgtgatttc agtgacaaat ggaaagttac
    42241 aaccattgtg ttctggatat aaaaagagta taataaataa agtatcttta tgtataaggg
    42301 aaaatgattt aaagctcaga agctttatag ataaaataga taaaagttat atatactttg
    42361 atgaagaaga tttattttta aatgtaaaca ctgttgatga gtataaaaaa ttaactaaag
    42421 tttaattagc tatggttgta aaatagagta ggatttatat ggaggagagt ttatgaattt
    42481 acttaaatgc gattcttggg cagtaatatt aaataatagc gataaagaaa gtaaagcata
    42541 taaaatatta gatgaactta aaaggaatat gtacaaagtt gttgctatag atgaagagaa
    42601 aaaacctatt gaaggaatag atgtgtatga atgtttaaaa gatgttccac acaatattga
    42661 tgttgtagct attattgata aacaatctaa gatggatgtg attttagaag aagtagagct
    42721 tttggatata caaaatatgt ggtttgaaaa aggaagcttt agtgaaatga tgattaaaaa
    42781 gactaaagat ttaaaattaa atatagaata taatttaagt ttatatgatg agttgactag
    42841 ataaaattat atttcttaat aataaaaata taattagtta ataaaacata ttattttata
    42901 gtatagattg gttaataaaa gtacagattg gttaatgaaa cattaaatta caatatttgt
    42961 taaaaaaatt gtgagttaca tgatataatg tattaaatag gatactaagg agttgatata
    43021 ttatgttaat agtaactaca gaaaaagtag aaggtaaaaa gatatcaaag gttttaggat
    43081 tagtgagagg aagtacaata agagcaaaac atgttggaaa agatatagga gcaagtttta
    43141 agaatcttgt tggaggagaa cttactggat ataatgaaat gctcactgaa gcaagacaaa
    43201 ttgccatagg tagaatggtt gaagatgcag aagctaaagg tgcaaacgca gtaatagcat
    43261 ttagactgtc ttcagcttca gttatgcaag gagcagcaga aatgcttgct tatggaacag
    43321 cagttgtttt agaagatgat aatagtattc ttgaaaaata aaatataaat agttaaaaag
    43381 aggcatcttt taaaaatttt atggatgcct ttttttattt gaaaaaacta atttgagaag
    43441 aaattttaac tattatgaca ataaaattag ggtgatatta
//
LOCUS       DAEOSP010000011        43231 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.011, whole genome shotgun sequence.
ACCESSION   DAEOSP010000011 DAEOSP010000000
VERSION     DAEOSP010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43231
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.011"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(183..2249)
                     /locus_tag="KRH54_000453"
     CDS             complement(183..2249)
                     /locus_tag="KRH54_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG8578132.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(2326..5355)
                     /locus_tag="KRH54_000454"
     CDS             complement(2326..5355)
                     /locus_tag="KRH54_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG8578133.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(5408..6868)
                     /locus_tag="KRH54_000455"
     CDS             complement(5408..6868)
                     /locus_tag="KRH54_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBG8578134.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            complement(6960..7334)
                     /locus_tag="KRH54_000456"
     CDS             complement(6960..7334)
                     /locus_tag="KRH54_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578135.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKVNKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(7510..8250)
                     /locus_tag="KRH54_000457"
     CDS             complement(7510..8250)
                     /locus_tag="KRH54_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG8578136.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(8271..9059)
                     /locus_tag="KRH54_000458"
     CDS             complement(8271..9059)
                     /locus_tag="KRH54_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBG8578137.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEISE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(9147..9237)
                     /locus_tag="KRH54_000459"
     tRNA            complement(9147..9237)
                     /locus_tag="KRH54_000459"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9201..9203),aa:Ser,seq:gct)
     gene            9496..10260
                     /locus_tag="KRH54_000460"
     CDS             9496..10260
                     /locus_tag="KRH54_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG8578138.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(10541..11656)
                     /locus_tag="KRH54_000461"
     CDS             complement(10541..11656)
                     /locus_tag="KRH54_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBG8578139.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLIFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(11697..13223)
                     /gene="glpK"
                     /locus_tag="KRH54_000462"
     CDS             complement(11697..13223)
                     /gene="glpK"
                     /locus_tag="KRH54_000462"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG8578140.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGIPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTNQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            13516..14613
                     /locus_tag="KRH54_000463"
     CDS             13516..14613
                     /locus_tag="KRH54_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG8578141.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMNLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPDDCYKFLDILKSKLEIKKVI"
     gene            complement(15104..15616)
                     /locus_tag="KRH54_000464"
     CDS             complement(15104..15616)
                     /locus_tag="KRH54_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578142.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            16169..17050
                     /locus_tag="KRH54_000465"
     CDS             16169..17050
                     /locus_tag="KRH54_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBG8578143.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(17363..18142)
                     /gene="hisJ"
                     /locus_tag="KRH54_000466"
     CDS             complement(17363..18142)
                     /gene="hisJ"
                     /locus_tag="KRH54_000466"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBG8578144.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKANL
                     NKYGEELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(18688..19038)
                     /locus_tag="KRH54_000467"
     CDS             complement(18688..19038)
                     /locus_tag="KRH54_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBG8578145.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(19054..19470)
                     /locus_tag="KRH54_000468"
     CDS             complement(19054..19470)
                     /locus_tag="KRH54_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBG8578146.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(19489..19680)
                     /locus_tag="KRH54_000469"
     CDS             complement(19489..19680)
                     /locus_tag="KRH54_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBG8578147.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(19831..20646)
                     /locus_tag="KRH54_000470"
     CDS             complement(19831..20646)
                     /locus_tag="KRH54_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBG8578148.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(20693..21631)
                     /locus_tag="KRH54_000471"
     CDS             complement(20693..21631)
                     /locus_tag="KRH54_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG8578149.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVNKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIS
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(21628..22500)
                     /locus_tag="KRH54_000472"
     CDS             complement(21628..22500)
                     /locus_tag="KRH54_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG8578150.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(22525..24453)
                     /locus_tag="KRH54_000473"
     CDS             complement(22525..24453)
                     /locus_tag="KRH54_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBG8578151.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(24565..24942)
                     /gene="gcvH"
                     /locus_tag="KRH54_000474"
     CDS             complement(24565..24942)
                     /gene="gcvH"
                     /locus_tag="KRH54_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBG8578152.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(25029..27155)
                     /gene="cdhC"
                     /locus_tag="KRH54_000475"
     CDS             complement(25029..27155)
                     /gene="cdhC"
                     /locus_tag="KRH54_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBG8578153.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(27212..28018)
                     /locus_tag="KRH54_000476"
     CDS             complement(27212..28018)
                     /locus_tag="KRH54_000476"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG8578154.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(28068..29435)
                     /locus_tag="KRH54_000477"
     CDS             complement(28068..29435)
                     /locus_tag="KRH54_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBG8578155.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(29466..30410)
                     /locus_tag="KRH54_000478"
     CDS             complement(29466..30410)
                     /locus_tag="KRH54_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBG8578156.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(30430..31200)
                     /locus_tag="KRH54_000479"
     CDS             complement(30430..31200)
                     /locus_tag="KRH54_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG8578157.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(31256..32641)
                     /gene="lpdA"
                     /locus_tag="KRH54_000480"
     CDS             complement(31256..32641)
                     /gene="lpdA"
                     /locus_tag="KRH54_000480"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG8578158.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(32685..33566)
                     /locus_tag="KRH54_000481"
     CDS             complement(32685..33566)
                     /locus_tag="KRH54_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBG8578159.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(33602..34276)
                     /locus_tag="KRH54_000482"
     CDS             complement(33602..34276)
                     /locus_tag="KRH54_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBG8578160.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(34311..35183)
                     /locus_tag="KRH54_000483"
     CDS             complement(34311..35183)
                     /locus_tag="KRH54_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBG8578161.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(35275..35907)
                     /locus_tag="KRH54_000484"
     CDS             complement(35275..35907)
                     /locus_tag="KRH54_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBG8578162.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(36012..37688)
                     /locus_tag="KRH54_000485"
     CDS             complement(36012..37688)
                     /locus_tag="KRH54_000485"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBG8578163.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(37937..38716)
                     /locus_tag="KRH54_000486"
     CDS             complement(37937..38716)
                     /locus_tag="KRH54_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG8578164.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(39026..40945)
                     /gene="cooS"
                     /locus_tag="KRH54_000487"
     CDS             complement(39026..40945)
                     /gene="cooS"
                     /locus_tag="KRH54_000487"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG8578165.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMTEKAEA"
     gene            complement(41552..42463)
                     /locus_tag="KRH54_000488"
     CDS             complement(41552..42463)
                     /locus_tag="KRH54_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG8578166.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(42579..43157)
                     /locus_tag="KRH54_000489"
     CDS             complement(42579..43157)
                     /locus_tag="KRH54_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG8578167.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIDI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTIVPGVTIGDNVII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
BASE COUNT        13343 a         8291 c         4770 g        16827 t
ORIGIN      
        1 ctctttcttt aataatacta aataatattt tccacattgt tgctttttct aaacaactat
       61 aaataatata ttaaaccctt atttcttttt ctaataagtt aaataataca ttttacacat
      121 atctatttgt cttgcacata ataaataata cattatattt ctatctaaat ttagattcta
      181 aattaaatag tataaattat attcttcaat aaacaagttt tcaaatccaa gattcgctct
      241 ctatctttaa actttattgt aaacataatt ccatcttttt taattatttc tccttcatga
      301 aattttttat ggtatacctt atcccctaca ccaatactgt caatttcttt tttcgttgga
      361 gattttatat cattaataaa taatgattta tcaactttct taccatactt atttaaagta
      421 gagcttatac ataacttttc ttcagctctt gtaatagcaa catacataag tcttctttct
      481 tcttctaatt gttcttcttt tttctcttcc ttacaaatat catatgattt ttcatgaggt
      541 attgttcctt catttacacc tataatataa acatttctaa attctaatcc ttttgcacta
      601 tgcatagttg taaatatgac accctcagtc tgcttattct tgttattttc cgttaactca
      661 gtctttactc tatcaatatg ctctaaaaac tctgtaacag tattaaaatt agttgctgaa
      721 ctctcaagtt catttaatat ctctacaagt ccattagtct ttatctttct gttgctacaa
      781 taatctaaaa catatctatc ataatcaaga ctagttctaa tataagatat tgcattttta
      841 ggatttaaag tatttaaata acttaaatct atttccaaat catttattgt tttaacttgt
      901 ttaggatgaa gattacactt atttattaat gctgttataa agtctttttc atccttaacc
      961 atatttatac tgtctttgga tatatatcta aaaggcttgt ttataattct caaccaatct
     1021 ttatttgatt ttggattaat acctattctt aaataggcta gtatgtcttg acttgcccaa
     1081 tggtcatata ttgtaattac tgagtctttt actacaaatg gaatcctcat atccataaat
     1141 acatctacta atgctcttga ctgtatatta gttctataaa ttactgcaaa atctgaatac
     1201 tctacataat ctttttgtat ttcgtcaatt atttccctgg caatatataa ggcttcttcc
     1261 tctgaatcat gcggagaaat ataggtaaca cttcctccat ccccctgact acacttaatg
     1321 attttttcat atctattttt atttttgtcg ataagtctat ttgcagtatg cactatctca
     1381 cttttagacc tgtaatttat atctaatatt atttttttag tattattaaa gtattgttca
     1441 aattcaagca aaaaatctgg tctagcacct ctaaatccat atatactttg gtcttcatct
     1501 ccaactgcaa atatattgtt taatgggcta caaattaact ttaaaacttc aaactgtact
     1561 ttatttatat cttgaaattc atctatcaat atatatttat acacatttct aaccatttct
     1621 agtgcagact tattgttcaa aagtaaataa taagttctta taagcatatc atcaaaatct
     1681 attttattaa ctttagattt ttgttcttcg tataagttgt atacttttaa aaactcatct
     1741 tttgttaata cttctgaatt aaattcattt ttatccataa gttcattttt tacataggaa
     1801 atttcattta taacttgtcc tatattctca tcatcatcag cattctctat atttaaactt
     1861 tttaggattg ccttgattgt cattcgcttt gacttttcat caaatatact gtctaagttg
     1921 tatctttcaa agtatcttag aattttaaaa aatacagagt gaaaagtacc ataggttacc
     1981 ttattaagtc ttatgtcatc acttaaattc aaagctctat ttttcatttc tattgaagat
     2041 gccttggtaa aacttattgc caagattctt gttggtgcta tgtttttgtt tacaaccata
     2101 ttggctattc tatatgtaat tactctggtc ttacctgaac caggccctgc aagtatcatg
     2161 caaggcccat ctatatgctc aactgcttct aattgatttt catttaactt tcttatatct
     2221 atatttgttg tgattattag ctccaccacc ttgatttata attatattat tttcttttta
     2281 attataaact aaaagttcat aaaaataaat ttaatttttc ctctattaaa ccacatcatt
     2341 cctattttca tgacatatct tgagttcttt tttaattttg tcgcttatat cttcaggtat
     2401 aaatgatggt ttactaatat agtagccttg catataatct actccaagat taattagtgt
     2461 ttccatttca ccttttgttt ctacaccttc tgctatcact ttaatatttc tttcgttaga
     2521 atatgagata agatttttta atattttctg tctgttttca tctttatcaa tacctcttac
     2581 aatagacata tcaatcttta catagtctgg cattatatat aatagtacag catctccgtt
     2641 gtaacctgtc ccaaaatcat ctaaagcaat ttctatattc caatggtcta tatatgcttt
     2701 ctttttctca ataaaatctt cattaatctt atcattctct gttatttcta gtactactct
     2761 atttaaatat tcaccatata aatcttcaat ttcctcaaaa tctacttttg acaaattatt
     2821 attaggaata gaatttaaga acagtttgca atcgccaaat ttttctttgt attttttaaa
     2881 tgatttaagt gctttaaacc atgtaatacg ctcaatttga tataatttag actgtgaacg
     2941 agctaatcta atgacatcta ttggcgaact aaaagttttt attttaggtc tcataagtgc
     3001 ttcatatgca aatactttac ctttacaatc tacaataggc tgaaatgcat actcaactaa
     3061 ttgaccatca attaacttgt ttaattcttc tctattactc aataagaatg aatccttatt
     3121 atatatatta atatcaaatt cactaacttc acccttgatt gtatttttaa tttgatacat
     3181 agcaaaatct gcgtatctca ttaattcatc atagttggtt gaatcatcag ggtaccagga
     3241 aattcctgct gatgccctta ttttaaattc tgttccatca tgaagtttta ataaggtatt
     3301 attcatctgt ttcttaacag aattaataat atctcttata ctatctttac tttcatatcc
     3361 atatataaat acataaaact catcacctga aagtctagat actataccat tccatgatat
     3421 aaacttctta agtatgtctg cagcacacct aatatattca tcaccagcat catgtccata
     3481 tgtatcatta atatatttta gattatctaa atcccacata ataaatgctg ctatcttaag
     3541 ttcatcttta ttcttaaatt tttcttgtat ctgtgaatga aaagctctcc tatttaaaag
     3601 attcgttaaa atatcaaaat ctctttcata ttctatcttc cttttctcaa taaattcttt
     3661 tgttacatca acaacaacac caagaacctt tagctcatct tcaaagatat tcattcttat
     3721 ccacttaagt tttttatctt tagattcaaa cttatatata tatgtatttt ctgtttcata
     3781 actttcttgc aaatatttat ctagtcccct taattttttc aggaacttat ttttttccat
     3841 atatccatat tcttcaaatt tgatttcagt agtaaacacg ctaaaaaatt cttttgtaca
     3901 aaaaacattc tctgcgtttt tgtataactc aaaagctcca attggcatat ctaccatttc
     3961 aataatctgt gataattttg acgaagaatc tgcaacattt gcactgagca actctatagc
     4021 tgaagataat tcatctattt cagatatatt tatcttatca agatttactg gttttgatgg
     4081 attactgttt ctaactttgt tggctaaaag aacaattggt tttgtaaata atcttgatgc
     4141 aatgtaaata cctattagac ctattaataa agaaattgct atagatatta taactaattt
     4201 ctctaccctt tttgaaaatg acaaaagact tttttcttca ataataccca aaagagccca
     4261 acgttcattt tcaaatggag tattggagtt atataaatct aagtattgta cactagcata
     4321 aatgttgcca ttaccacatt cattatcttt atttttaatc ttatatatat ttttatacga
     4381 ttccttctgc tctaaattag aatgtaaacc atcccaaaat ttttctttta atgagtatcc
     4441 atttgaaatt acattttcaa aggccatatc attatttcta tctacaccta aaatataatt
     4501 acccttttta ttcccattta tctctgagta tggaagtaat tctcttaagt aatttacact
     4561 taaatctatt ccaagtactc catatacagt tccatcttca taaataaggg gaactgtata
     4621 tgtcataagc ctatcacctg catttgtcaa taaaaactta cttccccagt atcctaaatt
     4681 agaacttgat acatttggat tatcaattgc agctctaaat ggtttataaa aaaaatcatc
     4741 tccatcttgc tcattttcac ttttaaatga aaacttaggt tcccaataac tgtccataga
     4801 tattccaaaa cttcttgcta tatctgatga acctctctca attaacaaat cagaattatc
     4861 atttggatta aacttagggt ctaaatccct tatatacaat cctgtcctac ttatttcacc
     4921 tttactatct attgacttat cgttaatatt tttgtcactt aggattataa aagctcctgt
     4981 aacagaattt tttctcaaaa gatatattaa atcatttgat atgcttttta taatatcttt
     5041 gttaatttct tcattgttgc ctacatcctc aattaaggca tttttttcat ttaatacttt
     5101 ttttacagag gaattaattg tagcttccgt ttcacttgta tttgaccatc tttgtaacat
     5161 ttcattttgt atataatttt tacgattaat tactctttca tttaagatgt caaacgaatt
     5221 atcatttaat ttcttaatag ttcctcccca taaaattgtg gcacaaaata aacttgtttg
     5281 aattagcata acaataatta ggggaaatat catctttttc attattgaat tgttttttct
     5341 tagattgttt tccataccca ttccacctat acctttgtgc tcgtcaacac actaactgcc
     5401 aattcatcta tttttttata gcattttcaa gctcagcctt aaaactttca aaccaagttt
     5461 taaaattttt atcattatta aattgtgcta ctgcatcctt cttagaagtt ccacttttta
     5521 ctaattcatt tattttattt gaatcttctt tagctttatc aagcattgaa tattcaagta
     5581 tttgtctagc tttagttcca ccatcaaatg ctttattagt atataaatta caactgttaa
     5641 tctgttcaat agatgaggct attgaacctt caactttatc agataaactc aaattatctt
     5701 tcttcataac ttcttttata acatccaaat cattcgtttt tttctttact ggtaagtatc
     5761 cagaagcaat agagaatttt atattaggtt ctttatctgt aaaccatttt aaaaattcta
     5821 ctgatgcata ttcttcttct ttagtagatt ttgttacagc cattcctgca ccttgttgta
     5881 cagcatattt tccttctcct ttaaaacgtg gtgcttgaaa aacagatgtt tcaatattat
     5941 aactctcatt atcatttagc attactctat ctggaaagta tgctgctcct gaactagaac
     6001 ctacaagagc taatatatca cctgtttttg catcatcaga acgaaactta ccataagaag
     6061 caaaatatcc atttatatat ggtacataga aattatccca tagttttttc ataacttttt
     6121 catcaatatt tagtgtaact ttaccatctt taacagaaaa aatttctact cctagttgtt
     6181 tacttcctat aataatataa tttgctaaag catctcttcc aaaaaatgct ttaccatcat
     6241 taggctcttt cgttaaacta tctgtccatt cataatattt ttttgatgtc tctactaatc
     6301 cttcaattgt ttttatatca tttatatttg aatttgtagc ttttgcaaat ttatcccaat
     6361 cagttttatt taacatcaaa atttcagttg attttgcaat tgggaaaagt ttaaactcat
     6421 ccttagaatt tattctgcct tcttctacat attcgtctat gtattcattt aattcttctt
     6481 tacttatgta cttatccata tctactagta accctaattt atccatttca tatgcagtat
     6541 ctgagtatgc tgcaaatata tttggtacat cttcagaacc aactttttta tttgcagaat
     6601 ctaaaacttt ctctgataaa tcaactacat taccttgact gaatgcttca acaataattc
     6661 ctttttcagc acctacagtg tcattaaatt ccttaacaag cttatcaaaa gccattttct
     6721 gctgtccatt ataataatgc caaatttgta ttgatgtcgg attttctggg tctaacttat
     6781 aattatcttc cttctttaaa ctacatcctg taagtaataa aacaatcatt gatgtgataa
     6841 ttaattttgc aaacttacac ctattcataa aatctactcc ttcaaattat aaaatttata
     6901 atagcattaa aactgtaact tcactattta tattaattat agaacgttta ttgatatatt
     6961 taaacaggat ttacaacata tttaaaagca attaagaaac atactattac aaatatagaa
     7021 aataaagctt ttacataatt tttatttcta aatgcgccaa atgacaccaa tccttgttga
     7081 atagctactg taattactaa aagtatcatt attatattaa gttgaaccag tttaaataag
     7141 taaagcaatg aaaacactac tagaagtaca cataatacaa tactagattt attaataata
     7201 tcatcaatct tatctacctt cttagattgc ttattaactt tatttaattt ttctttattt
     7261 aaattctcaa cttttttatt atacctttct cttgttttat ctttatattt agtattttta
     7321 tttttatttc ccatttattt tccatcctct ataaattttt taattgagct tatacattat
     7381 ttaaatatta tatatttaag ataccatata ttcctacaaa aaggtaactt actatgtatc
     7441 tatttttgat tttcgcataa aaaagccatc tcctttagaa ttaattctgt attgagacag
     7501 ctaatttaac tatttatcct ttgaaacatt ttctacatta tcatatttac tatcaactaa
     7561 aactatatca actcttctat tttttgacct accttcttca gatgaatttt ctgctattgg
     7621 tctaaactca ccatatccta cagcagataa tttatctggt gctatacctg cattgtcaat
     7681 aagaagttga acaacattgg ttgctctcat aactgataaa tcccaatttg acttaaactc
     7741 tgaatttttt atagacatat tgtctgtatg gccttctact ctcatgtaac tattcatttc
     7801 atttaacatc ttacctattt gaataatctt atctctagaa ttatccttca caatcgcttt
     7861 ccctgtatca aatagtattg tatctttaag gcttattact aatcctctat cttgaactct
     7921 taatgatact gaattagcca taccattttc atttaaatac ttctggattt cttcaccaac
     7981 ttttttaaac ttagcatttt gtaaagtatc taaatcatta cttcctggtt caactactgg
     8041 ttctatcttg ctattaccac cttcaataac tccagaagaa ccaccaaaag cagaatttag
     8101 agattgagat aattgtttat atttctccgc atcaacatta ctcatagaat acattattac
     8161 aaagaaaatc ataagtaatg ttatgaggtc tgaataagtt aaaagccatc gctcattgtt
     8221 ttcttctttt tcatcttcat catacaacat aaacattcca tccttttata ttattcagct
     8281 ctaccaccaa tttcttcaag ttcaattagt tcttttgaat ttaaaaatat ttttaatttt
     8341 tctgtcatta aattagaatt atcaccttca agtatggata atattgcttc tatcaccaaa
     8401 agtctttcac ttatttcctg ttgatttata gccttaagtt ttgatgctat tggtaaccat
     8461 attaaattgg cagaaccaac accatacaaa gtagccaaga atgcaacaga aatttgaggt
     8521 cctaatgcat ctggattact tgaaagatta cctaaaatat gtactaaccc cataactgta
     8581 cctattatcc ccagtgtagg tgcatatccc cctgctgaat caaaaattgc tattccttct
     8641 ttatgtcgtt ttgatgttat ctgtatctgt gattccaata tatttcttat agtaactgca
     8701 tccactccat ctaccataag ttcaagccct tttctaataa aagggtctaa ttcatcattc
     8761 tcacttattt ctttgtcaat actaagaagc ccctctttcc tagcctttgt tgcaatttct
     8821 ttgaaataga ttatattttc tatgttatta ggctttttat ttttaaaagc taataacatt
     8881 atctttatag actttttaaa cacagggaat ggagtcgaag ctccaacagc tcctatagtt
     8941 ccaccaaaca caacaagtgc tgatgaaaat acaaataatg ctccaacatg tcccccatca
     9001 ataacaaaag caactaccaa agatgctatc cccactatga aaaaaattaa agttgtcatc
     9061 tattccctcc tttctttagt tatacatatc taatcattta tgacattata aaaagactat
     9121 ctttaataaa aatagccttt aataattggc ggagaaggag ggattcgaac cctcgcaccg
     9181 gttacccagc ctaatccctt agcaggggat cctcttgagc cacttgagta cttctccata
     9241 tctctatact agtaaattat ataataaaat catattttag tcaattgttt tataaaattt
     9301 gagttatttt gaattattaa acatctattt ttactttttt gcttaaatgt aagttttaaa
     9361 tatggtaaaa ttatcttgca taacacttat aatattattt gataaatata catacattaa
     9421 caaatatacc taaactctga cttaattgta agttatgcca aaactaaaat aatactagtc
     9481 caaggaggat attttatgtt accacaggaa agatatgaga agattttaag tattctcaaa
     9541 tcagataata taattaaaat aaatgacata gttcaaatgt ttgatatatc aatagaaact
     9601 gcacgaagag atttagccca tatggagagt atcggtcttt taaaaagagt ttatggtggt
     9661 gctataccag ttatagcaag tagcgtagaa cttaactatg ttaaaagaaa aaaaataaac
     9721 tcaaacgata aaaaacagat tgctattaaa tgtagtgagt ttataaatac aaatgatact
     9781 atttttatgg atactggaac tactgtactt gaagtttcaa aaaatttgaa agataaaaag
     9841 aatctaactg taataaccaa ctcaatttta gttgcaaacg aacttatgaa tactaatata
     9901 aaagtatatg tgcttggggg ctatcttaga gaaactgaag gttctttttc tggtccgctt
     9961 acactgtcag ctattgaaca atttaatgtt gataaagcta ttttaggcgc tggaggtatc
    10021 actcttgaaa atggaatctc tgactatcac ttagaagaag cacttgttag aaaaaaaatg
    10081 attgaaaggt ctagaaaatc tatagtagtt gtcgaacctc ctaagtttgg tataaattca
    10141 tttgcatcag tagctcctat agaacatata gatactatta ttacctctgc tagtatagac
    10201 cataatataa taatagagtt ttctcaaaaa ggaatacctt tagtcatagc tgataattag
    10261 cttatatgat tgatttttag ttccttgatg taataaatta aaattaagga gccaaataat
    10321 tatttattta gctccattta tgtctaaact atactttata ttttaattat gtaactactt
    10381 tcagttagct tatcattttt agttatttga ttgccatttt atttaattta taagttttag
    10441 ttattcgatt attttttaat tgtctgtctt tttaatttat ttttagttat ttaaactcct
    10501 actttaaaat aatttaaatt tgacttaagt attgtttatt ctacactgca tctttcttca
    10561 ttttaatact ttgttcttct tccaaggtta aatcttttgt cataagtata gcaagtgcaa
    10621 ttgccaatac tcctccaacc aattttccaa ttacaacagg tgtaatcatc tctggaacta
    10681 ctcctgctgt aaatcctaaa tggtccccca aagctgctgt agcacaaacc agccaagcag
    10741 tatttactac tttccccttt ggactcatat ctttcatcat tttatatact ggaatactat
    10801 tggctaaagt aaaaactaat cctgctgcac tagtggcatc catacctaat tttccaccca
    10861 ctattgtaag tggtttatta agaactttaa ctaataaatg taatattgga aaagtaccta
    10921 ataacacgat tgcaatactt ccaattactt gaataccttc tcttataggt gtcatgcctt
    10981 ttataagtac tatacctgtt atttcttgaa atgctgctgc tcccaaacct atcgttatca
    11041 atatagatat aaattttcca aaaattaatg acccatttat cataagcgta gaattaaatt
    11101 ttaaacctac tgccaatatc actgccaata ttatcactgg tatcatattt ataattacca
    11161 ttgataagtt aaatcctgct attaatcctc ctataatacc tccaaaaggt attgttatca
    11221 agcctatcag taaccctttt gcaaagaatg gtctgtcttc atattctata agacctagtc
    11281 ctactggaat tgaaaatact aatgtacatc caagcatgga tgacacaacc aatcctgcaa
    11341 gcattcctgc ctctttattg tgtgcaagtt ccatagccaa tggataccct cccatatcat
    11401 tcgctaaaat agttgcaaac atagatgggt cagctccaca gagattgaag attggtatta
    11461 ttataggacc tagtacatct gaaattactg gagctaaaca aactatacca accattccta
    11521 aagccaatgg acccattgaa ttaaaccctt cttcaaactg ctctccaagt ccaaatttat
    11581 ttccaattat tctatctaac gctcctaaaa gcactcccac agccattatg tataatataa
    11641 ttttatcaaa gggcattttt aattcctcct atacatgtaa aatactctat tgtaacttaa
    11701 ttagcacaca cctttttggt atcgttttcc caattcatag ctctttttac tgcttgattc
    11761 catccaagta atatttcttc tcttttttga ttagtcatat ttggaatata ttttttgctt
    11821 attgtaaatt cacttctaat ttcatcaata ctgttccaga caccacaaga aagacctgcc
    11881 atatatccta ctccaagtac tgtcgtctca ggattttctg caatttcaat tggtatacct
    11941 aatatgtcgg attgaaattg cattgtaaag ttatttttag taattccacc atctactctc
    12001 aacattttta tttttttccc tgaggctaag ttcatagaat caacaacatc tttgacttga
    12061 agtgcaacag actcaagagt cgctcttact atatgctctt ttttagtact tcctgttatc
    12121 ccaaccattg tacctctagc atattggtcc caataaggtg ctgctatacc tgcaaatgct
    12181 ggtacaaaat atacacctcc agtacttgat atggaatttg ccatgtcatc agtttctgaa
    12241 taatttttta taactccaat gccatctcta agccattgta ttgcagcacc tgctatatac
    12301 actccaccat cataagcata tgtaacttta tctcctattt tccaagcaac agttgtcaaa
    12361 ataccattac ctgctatatt tagttcctca cctatattca tatataggaa acatcctgta
    12421 ccataagtat ttttaaccat tcctggttca aaacatccat tgccaaataa agctgctggt
    12481 tggtctacca ttgaagcagt aatttttata gaaaccccac aaaatgcctc cttatctgta
    12541 gttccaaaat caccactcgt aggcattata tctgctaaaa tacttctagg tattcctata
    12601 atgtcaagca actctctatc ccaatccata tctcttatat taaaaagcat tgttcttgct
    12661 gctgtagata catcagttaa aaaagatttt cctcctgtca ttttccatat taaccaacta
    12721 tctaatgttc ctgcaagtac ttctccattt ttaatttttt cttttacacc ttttacatta
    12781 tctattaccc atttaatttt tgtacctgag aaatatgggt ctataattaa cccagtctta
    12841 tctattatct tattttctaa acctggtata ttctttaaat tttccacata atcataagtt
    12901 ctcctgcact gccacacaat agcattgtaa agtggtttac ctgtattttt atcccataac
    12961 attactgttt caccttgatt atctatacca attcctttta tatcactttc attgattcca
    13021 ttttcaaaag cctgttgtaa tacattatta ctcacagttt ttgtattatt ccaaatttct
    13081 atagggtcat gttcaacata tccagattgt ggatatattt gagtatgttc catataatcg
    13141 ctaaaaacaa actctgattt ttcgtttatc aatactgctc ttgttcctgt tgttccttgg
    13201 tcaattgcta atacatattt cactttaaat gtcccccttt attttcagaa ttaacaaatt
    13261 cttttttatt ttttgaatat ataatctatt caagttattt atattttata tcatcatgtg
    13321 gatttatgca atattcatta caatattttt tgttattttt aattatagtg attaaaaaag
    13381 tatttttatt gaaatattat tgcaatattt attattttat tctataatct agttaaaata
    13441 acacataaca ttttaatatt tacacataat cagtcattca aaaatttaaa tttaactttt
    13501 taaggaggct ttacaatgaa aaaaacatat ctattcagtc ctggaccagt aatggttaca
    13561 gataaagtca gaaattcact tcttcactat gatatatgcc atagaggaaa tgagtttatg
    13621 aatttattca aagatacaca acaaaaaatc aataaactat ataatgcaac ttctaattac
    13681 tactcattaa ttgtatctgg ctctggaaca tctgtaaatg aatgtgtact atcttctatc
    13741 tttgacaaag aagatgcagt tctgctagtt agtaatggtg tttttggaga aaggctagaa
    13801 gaaatattat ctacttataa tgtaaaaaca tataaaccta attttgaatg ggcagaatac
    13861 ccggacctag acattttaga aaaatacata attgaaaatt ctgatataaa agtaattgct
    13921 atggtgttcc atgaaactag ttcaggaatg ataaatcccg tccctgaact aggaaaactt
    13981 gctaagaagt ataacaaaat attttttgta gatgcaataa gtgcctcagc aggtgaatat
    14041 attgatgtgg aagagtttaa tatagatata ataacaggtg ttggtggcaa agctcttgga
    14101 gcatttccag gttctgcata cctttgtgct aaagagagta ttttacaaaa tattaaagaa
    14161 aatcaatgta agaatgtgta tcttaatctt tataaacact ataaagttgc taaatcatct
    14221 tctcaaacac caaatacacc taatgttact ttaatatttg cccttaatga agctttaact
    14281 gaatttttgg aagatgacag taaaatagaa agatacaaag aatgttctgc tattttaagg
    14341 aacggtatgg aagaattggg tctaacattt ttacttccag ataagtatat gtctaataca
    14401 gtaacatctg tatttctacc taaagaaatc aatgtaaatg agtttatatt agggctagaa
    14461 agggaaagtg gctatgttgt ctatccagga aaaggcaaat tcctagatgc taacatattc
    14521 caagtagcaa atatgggaga aatatatcca gacgattgtt ataaattctt agatatctta
    14581 aaatcaaaat tggagataaa aaaagttata taacagtttg tttgtaaaat cctacaatat
    14641 atcagtataa attaaataga ctgatttttt taaattttcc tataataaat tatattttat
    14701 atttttgtat aagggtgaac tataatatgt aaagttcacc cctatttcaa atctttttcc
    14761 ttcaatataa tctttttctt ttaatttatt ttatattttt gacgcaatcg ttttttattc
    14821 aatcatttta tattcttgat acaatcattc ttacacttaa ttattttttg tttttgacac
    14881 aatcgttttt tgttgaatca ttttatattc ttgatacaat cattcttaca cttaattatt
    14941 ttttgttttt gacacaatcg ttttttattc agtcatttaa tattttcaac acaatcattc
    15001 ttacattcta ttattgtttg tttttgatac aactgttttt attcagtcat ttaatattct
    15061 caacacaatt accttttcat tcaatcaaag taatctttaa gcattatttt tcaagctcat
    15121 caaagtaatt atatacgata acctcacctg tctctttatc tgtagtcttt ataatgccct
    15181 tagctccatt tacctcttct tttgtaaatt catcatctaa cacaactttg aatttatctt
    15241 cctttccata accttgttga aattcatctc tcaaaattgc atttttttct gcttttggct
    15301 tcaactcaaa ttcaattcca gatttcattt catcaaaata atttaatact acaacctcat
    15361 tattcatttt attagttgtc tttataatcc ctttagctcc atttacttct tcttttgtaa
    15421 attcatcatc caatgttata ttaaatttgt cttcttttcc atattcctct tgaaaatccc
    15481 tcttcatttc attgtattta gtttcaaact ctttactctt gtttaattcc atagctgata
    15541 catttgtcaa tgatgctcca attgccatta ctgttaatgc cattactata ccacttttaa
    15601 atttttttac tttcatatct tacctcctaa attaaatgtt ctcaatgaaa ttatataaag
    15661 aaaatcctaa gttttacagt ttcattcttg taactaaaat tactattatg taaccaaact
    15721 ttttgcttca actattcctt aaaagttgct tttttgagga atagttgaag ttaaataatt
    15781 agattttatc tatatttata gatttaaaat caaatttatc catataaata aatacttatt
    15841 tttgcaaaat aaaaattttt aattactcca atccttctta tttacttaaa attctatgat
    15901 aagaaattta aaagtcttta ccttctttaa caataattta tttactttta acatacaaaa
    15961 tagagagagt atatcaaaat cattttactg aatattctaa aaataagtac cccctattag
    16021 tatatatatt tacatattaa aaaaaagttt taaataaagt tatatttttt acaacagttt
    16081 tgtaattaaa aacatattga aggtaaattt aaaggtacta tatactataa atatgtcgac
    16141 acaataaatt taaaaaggag aactaaatat gaaaaaaaga ttaataataa tgatattgtc
    16201 tgttgtctta gtactatcaa gtatacttac gatatttgca tattcaaata ttaaatataa
    16261 taataacaaa ttgatatatt ctaatatgat agataaaaaa actcaaaata gtgtaaagga
    16321 aatcctaaaa gaaaataaaa ttaatgaaaa agacatagat acatttatta aagcagtcaa
    16381 caattataat aaattgcaag ttaaaatact tcaaaataat ataaatatat ctaaatctgg
    16441 atattcttca atcaatgcaa aacaagttcc atataaccta gaaaaattgc aagataattg
    16501 ggtgaaaaaa ttccctgatt atatggatgt aaattgtaga ataactgctt ttagactttt
    16561 taaagatttt ataaattcaa ataagaaatt tactggagac tctattgatt tgaatgtcga
    16621 cttggacact ataatgaata ataaagatgc taaatttagc accaaagatg tagaaaaatt
    16681 tattaacttc ttttctgcaa taccagctaa agacacagat gatacaatca aaattgcaga
    16741 acaaataaaa aatgaatgga aaaaaagaaa aatatctttt aaaaataata aaaatatatc
    16801 tattataaat ggatttcttc gttatccaga aactaaaaac gtatttatag gtcatacagg
    16861 catatgtatt aaaactaaaa atggcattct atttttagaa aaatatggtg ttacatcacc
    16921 ttatcaagtt actaaattta aaaataaaga agatgtaaaa aattatatgt ttaatagatt
    16981 gaagatgtca gaaggtgaaa ttgaattacc tgaccctata ataatggaaa acgataaact
    17041 tatgaaataa taactttaaa cgcaaaatag tatttataat tactaagtca atataataga
    17101 gaaggtctat agatttgtgt aaatacaagc ttatctacaa tcttatataa gaatgtttat
    17161 ataaataaga tagattaatc ctaagtccta ttattctatt gaattttatt attattttat
    17221 ttagattcta ttgttatttt acttagaact tattattatt ttatttaagt tctattacta
    17281 ttttcaatca tataaattta caaaagactg tacataaatt tattttatac aaacttatct
    17341 acagtctttt gtgtgaatat atctaaataa tatcgtattt atttatttcc tctgcacagt
    17401 gagaatcaga acctaaaact aatggaatat cgtatttttt taccaactct aataagtatc
    17461 catctatata aggctctttg cagtactctt ttctatttcc agaaacatta taatcaagtt
    17521 cataaccttt ttctttaatt aactttatta actcttcaag aattgtattt cctttataat
    17581 cgtatggaaa tacctgatta aactttctaa ctaaatttaa atgacctatt ctttttggtt
    17641 tatgtatacc taaatctgaa tttacagctt ttatcaatgt ttcataatac ttattataaa
    17701 cagcttcaat acttcctaac ttgtcgatta aattttgaaa ctcctctaca ctgtaatcta
    17761 cacaataata atcatcatca attttaatta tatgtactga taaaagagaa tcctctaatt
    17821 cttctccata tttatttaaa ttcgccttta tcccttcttc gtatccttct ataaaatcca
    17881 cttccgaccc aacatttatc ttgattttac cctcatactt ttctttcaac ttcttgacat
    17941 cattaaaata ctttggtaaa tcttcaatac tcatacaact atcattttca ggagcagggt
    18001 ctctaaatcc atttgcatac ggaaaatgtt ctgtaaatgt catttctgtt attccaacat
    18061 ccattgctct ttgtacatat ttttcaaaat catccttact tccatgagga caataaggtg
    18121 aatgtatatg accatctttc ataaataatt cactcctttt ttaaagaaaa aatctaatat
    18181 aaatgcataa atcttttaac ttcaaaataa tatttttaat catgctaatt tttattatat
    18241 ctaatattag agtaatttta aactgccttt taagtatctt atcattagta ctattttcaa
    18301 cttaacatta atattatttt ttaacttaac attaatacta tctttaaata gtaatactca
    18361 atatataatt acttaacata aaataactca aattgcttga tgaggctttt gcagtattta
    18421 aagaatatat aattatttaa cattaaatta tccaatcttg taataaaatt tactcattaa
    18481 agtatattac agaaaatacc tcaaaataaa ctttttagtt catgagatac tcttttttac
    18541 atgaaattag atatattttt atcatttcaa caatgaaagg gaggcttata tccatcttta
    18601 gacaacccct tagataaaat ttttaataat taagcatcac aaaatataaa aatcgcttat
    18661 atcaaacgta taagcgatta attatattta catatgtttt ttagcctcta acaacatttt
    18721 attagcttct tccatatact ctatagcctt ttcaatactc tttgctgttt cttcttttcc
    18781 aatagctcta gccttttcaa cccactctct aaatccttct tcatgagttt cattgtgatt
    18841 tatccaatgt actaaaagta tttttaaagt tttttcatcc ttagactctg atgattcctc
    18901 atgactatgt tcgtggtcgt ggtcgtgagc atggtcatgt ggatgttcat gacatatatc
    18961 tccatgagta tgttcgtggc agtggtcatg gtcatgatgt ccatgatgat atcttacaat
    19021 ctctttatca aaaatcatta gttattcccc ctgttatttt atattaaact ctttttttat
    19081 aaggtttaca gaatctttta caagtagtgc taatggttta ccttctaatc caactaactc
    19141 cacattttct ggcataatag gtaaaagtat cttaagtgcc tcactgtctg caatagcttc
    19201 acttatatta caagtaactt ctcccatcat agaattaggt attattacag aaataggtgc
    19261 aactattata tttgcttttt tagctgatac tactatagca ttttcccctg tagctccttt
    19321 attagcatga gctttcatca tagaactagt tgctatagaa ttagtcccta gtgcataaat
    19381 ttctacatat gtaggtaact cttctctcaa agaagaaact atctgggctc caatacctcc
    19441 acccattcca tctatcactg ctattatcat atgaaattcc cctattaatt aattttcttg
    19501 tattattatc ttatggtcca tcaatcttat ttctttaatt atcccatcta ttatcttttg
    19561 ctctcctaat aaatccgtta aataaatctt tccatcacaa gggtctatat taaccacatt
    19621 ttccataacc ttttctaact cattattact tttacaaatg aaagctgatg attcacacat
    19681 ataaatctcc tcctaatatg atttttatta aaaatcaaaa aaagattccc ttaggtattt
    19741 caatacacca aagaaacctt catgatttca gtttttgtat attattatac cttaagtata
    19801 tcactttaat ttatgtaaag tcaattaaag ttaacgcttt ttattatact cttcattctt
    19861 gtatttttca tatagttctt taactaattg cttctcataa tcagtcaaat cgccctccac
    19921 aaactcaaca ttaaattgtt cagctaatcc ctttactatg tttttctgta gaatatctat
    19981 gtctattttt ctgccaattt cattttctat tccacttgct tttttagctg taaatttcat
    20041 agctctttct ttcaattctg gtgtcttagt ttttattaat ttaaacaatt tttctacatc
    20101 aaaatccagt attattgaac catgttgtaa taaaacacca tcttttctgc tttgagcact
    20161 tccaattacc ttcttattat taatagtaac ttcatatgat gcatgtgcat taaaacatgc
    20221 agctgataaa ttttccctac ttattctctc gcctctattt aaattatctg tttctatacc
    20281 acttagattc aaccctttaa ctagaccttc acttatatat ctataagata ggttaatgct
    20341 cttatcgatt aaaggattat cttctcctat aattatacta taggttagct cattatcatg
    20401 taatacagct cttccacctg taattcttct agtatagtct atattcatac atctacactt
    20461 atctaaatct atctcatctt ccaatttctg aaaatatcct atactcaaac aagctggttc
    20521 ccatgtataa aatctaagag ttggcttatt atgtcctttc ttatatgcag taaatatagc
    20581 ttcgtcaatt gccatattca ttgctccttc atatgattta ttatgtataa ctctccactg
    20641 attcatgatt attacgcccc catttttagt ataaatatat cctagaaatc ctctatagat
    20701 ttatttctct tattgctaaa tctatctcac tttgatttaa aggtcttgaa tagttgtata
    20761 tagtacttcc tggtctttca ttatagtatg gtctattaca atccttacaa cctcttattt
    20821 caaaaggata gcctttgttt atgtctttta aaatatcatt gtcaagcttt atactattta
    20881 agtatccatt tttaaactta aaatactctt ttggataacc tttatttatc atataagaca
    20941 ttaactgtac ccttctataa ctttctatgc ttggttgact tatcttttcc attttagttc
    21001 ctctaactgg agtaaatgca aataaactaa tcgttacatc attttccttt aaataagtat
    21061 ataaattgta tatatcttca tgactttcac ccataccaac tattatatgt gtacttattt
    21121 tattgggata tgattttgac atttcagtta taaattttaa cttttcatca tagttatttc
    21181 cttttatttt ttcatatagt tctttatttg ctgcatctat tgctattcct attttattta
    21241 ctcctgctga aaaaaacttc tttatctgtt catcattttc taattttgca gatagtgaaa
    21301 ttggcatgtc tatcttacca cttatatagt ttataaagtc taaaacagag ttatgtgctt
    21361 cttcacttgc cattgattga atacatattc tctttatatt ttttccttta taagctttta
    21421 aagcatctaa tatttcttct tttgaaaact ctggccaaat tactcttgat aatttatctt
    21481 ttctagtact actttctatt gattgagaac aaaaactgca tttatttatg catttttctc
    21541 caatcattat atatgctgtg gtaggaggta tatcgctttt tttgtttaat atcccaagtt
    21601 ctattgctgt cccaactgat agtctaatca taaaacgcaa cactccttac actcatttat
    21661 tgtcatattc atttctatag ccttattctt tgcagacctt gatggtaata ctattctatt
    21721 gactccacac attatagata attggtctag ctcttttcta tacactcctc ttggtctcat
    21781 acaacctaga ttaatttcag tatctggtaa attaattcta gcttcacata aaatatctgc
    21841 taccccttct aactcaggag gttctacatt ttcatactca gtcccttttg ttggaataag
    21901 gactataaat gtaagcttat taggaggatt tttttgtagg tattctataa tttcatattc
    21961 accctttatt tgtcctcctt ttaaaccaat acatatatgt ggtgctactt ctgtatgttc
    22021 ttgtattgta ttatagactt caatataatc ttctttactc tttttcatct tataaacttc
    22081 tcttattgtc tcatcatcaa acactaaatc aaaagaaact atatctaaat acttacacaa
    22141 ctctacaatg ctatctttat ccataagacc taaatgagca ttcaatctat atccttgctc
    22201 ttttaagttt ttaatggtat taatatgagt atttataggg acatctccct cataactaca
    22261 accgccactc aatagaaagc tagttatatt ccttgattgt actttttcct catattcttc
    22321 tattggcacc atattgttta agtagtgacc gttgcaatgg gcacatttca aactacattg
    22381 attgcttgta gtactaagag ctagtgtctg gttaggataa gcaaactcta ttttatttcc
    22441 aaaattcttt aatttaatat caaatgctat tttcattttt tcagatagat tcttattcat
    22501 agtttattcc ttcttaagta cattttactc ttctagtaat ttctttgctg ttgggaactg
    22561 ctctatattc gtatgtggta agaaacatgc agaaataaat tcatccatat aacttggata
    22621 tacactaagt tcaacataag ttatttgatt acctatttct tcaagtttat tttttgcatc
    22681 tttacttata agagctaagt aagaaccaac aagtgaacta ttccctatat aagtaaattt
    22741 ttctctatcg atatcaggaa gtaaccctat aattattgag ttttctatgt ctaggttgtt
    22801 tcctatacca cctgctatat aaaccctatc taatatacta aaatccatac ctaaactatc
    22861 tacaagagtg tatgcgcctg aatatatagc tcctttagct ctgatgaaat tatctatatc
    22921 aacttcattg actactatat cattttctaa atcaaattct tctttaaatg ctaatacata
    22981 ctctcctatc tcatgctcat taaatctaac tctcttatta tctaaatccc tgtatatttt
    23041 acctcttcta tctattacac ctttagtaat catttgacag attaaatcaa ttataccaga
    23101 accacatata ccaactggtg cacattcatc aataatcttt aatgttggct ctaaagtatc
    23161 tttatcaata attactttct ctattgctcc tgctgatgct ctcattccac agcttatccc
    23221 tccaccttca aaagcaggtc ctgctgaaca tgcacagctc atcatataat ctttatttcc
    23281 aaatacaatc tctccattag ttcctaaatc tataaataaa acattttctt cacttgacca
    23341 tatccctgct gataatactc ctgctgttat atctcctcct acataacttg caacagatgg
    23401 agctaagtac acatatgcac tgtcatttac aaaaagacca acattttcac ccataagttt
    23461 tggagatttt aaaaatggtg gtatgtaagg ttcttgtctt aagaagtctg tatacacacc
    23521 caagaataaa cttgtcatag ttgtattccc agcagccact aatgtaacta catcttcctt
    23581 atctattcca tttctttcat atattgtttt taatagtgga tttatagttt cctcaactat
    23641 agctttatga agtgtctcta atccattttt cttagttgaa tatataattc tatttataac
    23701 atctgctcca tattttatct gtgcatttcc agaagatgct ttatctacta cctcttttga
    23761 gtataggtct actaaacaaa caactactga agttgtacca atatctattg caactccgta
    23821 taaggaattt tctttatttc cttgttctat atttatgatt gtaagtttct tttgtttttg
    23881 aacatatgtt attgtaacct taaaatcact ctttctaaat acagttggca tttttctaag
    23941 tatatccaat ctaaaatcta tttcattata tcctaggtta tttctaacat atctttccaa
    24001 tctatctaca tcacttatat tatcatctaa atttggttct tccatttcta tgtatttctt
    24061 ttttatattt gttttaaatt gaaggttatg ttcttcaatt atttttttag ctctttcaaa
    24121 aatttcccta tcctcttttt tattactacc ttctatcttc ataccatgca tagaggatga
    24181 tactttagaa ggaacttcaa tttctatatc tgatattacc tttgtacaac aagctagtat
    24241 ataaccttgc tcccactcat catctgttat atgtcttgtt ttttctgtat caacttttcc
    24301 attcaataat tttactttac acttcccaca agacacgttt ccattacaag gagcatctat
    24361 gaatatatct gcatttcttg caacctctag taatatatcc ccctcattac aataaacctc
    24421 tttattgttt ggtgtaaaac ttacttttat catgttatcc ctcccaattt gttctatata
    24481 tgataaaagg aaagtatagc taaatttgcg tgcactcaaa ttttactata ctttccccaa
    24541 ttataataat atcttaatct ttaattattc taatccagcc tcgtattctt tatcacttaa
    24601 taacgtatca agttcagcca catcagctag ttttactttt aatatccagt tttcatatgg
    24661 gtcttcattt atacattctg gctcatcttc taatttttca ttaacttcca atacctcacc
    24721 attaacagga gatattaaat cagaagctac tttagaagac tcaactactc caaaatctac
    24781 tcccttagtt acagtatctt ctacttctgg tgtttcaaca aacaatattt ctcctaattg
    24841 atcttgagca tagtctgtta tacctatata tacaacatct ccatcaatta cttttaccca
    24901 ctcatgatct ttagagtatt ttaattctgg taataatttc atttcgatag acccccaata
    24961 ttatattatt gattaaatta cttaaatcat attaatatta tatcatatta ttttaaattt
    25021 tccatcaatt acattacact ttccatagct aatgctggat gacctttttc ttctaagaaa
    25081 gctaatactc cttcagaatc gtcagctata gtttcatcac aaaccatatc acagaagttt
    25141 tctattccag tcatttcttt aactgtagca tttaatttat cagctacata atcttttaat
    25201 tcttttggca tccaaactat tctttctggt ccaccttctg cataagcgaa ttttttagaa
    25261 gatatgaaat gtcttccgtg tcccataaat cctggagttt gaactcctcc acctgtcata
    25321 gaagcaagtt ctccgaaagt cataccaact ggagtaacac ttgcaaattc tctgttaact
    25381 acaacaaatc catttgcttc tggcattata ccacagatac attcgaagca tccacaagaa
    25441 gtcattgggt cttctagtat agagtataat gtaacagatt caactgcacc ttgagatatt
    25501 tggtttacag tttcatttac tgaattccat acacctgttc tatcatctaa gcactcgcct
    25561 ttttctattg gttgacaagg acctgttggg tctaattctt tagtagcctt agcatctaac
    25621 cagctaactg ctccacaaag accaagtctt tcaggagtaa caacacaaac gtgagctggt
    25681 gcaaatgatt gacataagtt acaagaatag aaagtgtcaa cactttcatc aactaatgaa
    25741 gctaatcttt catctctagc attgtactta gctattgctt caccttttaa ttcagaaact
    25801 ttttcagcat ctgtaattat agttatttca cacttatcaa ctactgattc aaattcatct
    25861 aacatcttag catataaaac ttctcctata tgctcaagtc taaatccttt atcaaatgca
    25921 tctttagaga ttcttaccca agccatatct ctttgtccaa cgtgcattac accttctata
    25981 tagtttaaga agtagtggaa acgtctttca agaactggct cgaaatcttc ttgcatattt
    26041 ttaccagcta ttttaactat tacagcaagt ggtaatctta atacaccatc tgcatcaact
    26101 tcatctatat ttggtcctat tatttctatc ttatggtctt caacttctgt tgcttctttc
    26161 ttaacaacaa gctcccaagc ttcagttctg tttccaccaa actcagcaaa catatcgctc
    26221 tttctaattc tctcaccttc aaatgctgct gcaaatgaaa ctgggatagg tatttcagta
    26281 acttttattt ttattcctct agcttctaat gaagtagcta ctatcttatc gtaatctttt
    26341 tgagttaata ggttcattgg aacttcaaga actgtttggt cagttataac tgggaatcct
    26401 aaagctattg ctccagcacc tgcagataca actaattcac ttaatggtcc aaatgcatta
    26461 acaaatgcag gaactctatt agctgtgtac tctaataatc cgtttaaatc accaggagtt
    26521 aatccaccga atattaatgc tgctcttact gcaacagata caacgtgtat aacagaagtt
    26581 acatcatatc ctagaggtat aactcttagc tcaagaccca tttttactcc agcttctata
    26641 gcttggtcta taactttacc aactaagaaa gttagtaaac ctttagattg gtaatctttt
    26701 ataacttttg ctgctgattc tgcatctgga cactcaccaa gaacaactgc aacaccaggt
    26761 atatctcctg taactaatgg tacaccaagt gatctgatta tagggtcagt tatatgacct
    26821 gcacatggct cactatatgg agcatccata gttgaatatt ttaatgcttc aattacttct
    26881 gctgctaaag ctgtagctaa accagcatta aaagcatgtt ctagtaaatg agttctattt
    26941 attaaagatt ttactacttc taaagcacct tctaagtccc ctaaagtgtt catcttttgt
    27001 cctgttgcag catatataca aggtaatgaa tatgctgtat cagggaaagc aactttatgc
    27061 tcttttccat tcttttctat agcttcagct aacatagctt gagccgcacc taaagcttgt
    27121 tctgaccctg taaagattat attatataga ttcattctat ttcccccttt aatatttaca
    27181 cctatttttt attaactata ccctaaaaac cctagaaagc aaatccacct tcatttaact
    27241 ctaagaaaga gtctgcatat aaagatgccc cgttgattat gtcacaagat tttacagttt
    27301 tcattaattc tgggtcacat ggattcatta tagctgaact aaatccatta gccattgcca
    27361 ttgctaagaa tgcactatct aaaacaggtc taacatgctt aggacatcca ttagatacgt
    27421 tagataatcc tcctgtagtt aaaagtccca tctcagtcat catttttata gcttctaaaa
    27481 cttctacttg tttttcttgc atacctttta taactaagca taatgggtca aatagtatat
    27541 cagttggctc cattcctaac attaagcctt tttctaatat ttcttggcag taagccatac
    27601 gctcatcatt atctcttggg ataccttctt ttgcacataa acctataacc attgcttcgt
    27661 attcagctgc tatatctatt aaatcaaatc ttgagccagc atcagcagag tttattattg
    27721 gttttgcatt agttctatca taaactttaa gtccagcttc gatagctttt ttattagctg
    27781 tatctaatgc tataggaaca ttgttgaact cagattgaag taatttgatt ccccatgtca
    27841 ttatttcttc gccatctctt tcggcaggtc ctatattaaa atctatatag tgtgctcctg
    27901 cttctaattg ctcttttgct ctttttaaga ttggagctgg gtctctttct gctaatgctt
    27961 ttcttattga aggtgatata caatgtattc tctcacctat aatcataaat ttttccattt
    28021 ataatgtccc cctttaaatt taatattaat agttagttaa tattttatta gtttgtttgg
    28081 catgcttttt cttggtatcc ttttaagaac ttaggtaatt ccatagattc ctctggtcct
    28141 ataactacat tccatcctgg taagttatct tctatatcac ctttaagtac tgcaactttt
    28201 cctggaatga taaggtcttt acaattagtt acttcttcta ccttactttc tttaatgaaa
    28261 gcagagattg agtttccacc aaatttacca gcagcccaag atgtaagaac tgaatatcca
    28321 cctgcatctg gtattactaa ccatactgga acttttgatc tttctatatc accagcaact
    28381 atgaagtaag ttaatgcaaa gtcaacagta actaatactg gagaattttc atctgggttg
    28441 ttgattggat aaatctttgg ttcaactctc attggtcttt gtgggtcagt gtatatgttt
    28501 tgtcttagtg caaataatgg taatgcctta gcataactaa tatcatctat tactattata
    28561 gaaccgtatt ttatagtgaa tattgatgat aaagcaactt ccatcatagg atttgaatta
    28621 gataatctat ttgcaaatac tattgaagga tatccaaatg ttctatcttg ctcttttaaa
    28681 gctgttcttc taacttgtat agcattagtg taagtatctt ttatgttttc tcctgttaca
    28741 tctaatacta attctttata tccagcagct tgaattaatt caactgtttc atataattct
    28801 tctaagctac cagcttttac acctaatggt aaacttgctc ctttaactac ttctatcata
    28861 tctttgtagt tctctttagt tgctccatat accattggtc tagcatcttt taatacttca
    28921 acagcttctt taactacttg tgcatcatca caagctaaaa tataagctac ttctaatcca
    28981 ctgcctttta ttttatctat taattttaaa tacgcatctt tatctccaca atactctaaa
    29041 acagccattt caactttcat ttgttcacca attcttacat agtcaacttt tttcatgtta
    29101 gctattttag catctactgc ttcatcactc atacaagtac agaatgaaac tgcatatcta
    29161 tttctactta ctaatgtttt ttcatgtcta aataatactg tttctccacc tagttcatac
    29221 tcagatgcac cagcaccaac ttttaaagct ttcattaaag gtgcagtagc ctctgataat
    29281 tttgctatag cgtcatcaga catatgtggg catttaccta cttctacagc tcctgaagca
    29341 accttcatag agaaagccat acaagttggg aatccacaat ccttacagtt tttctttggt
    29401 gttaatttaa atatatctaa agcttttaat gccattattt tttcccccta accttaacaa
    29461 tttaattaag ctaatgcgtt aaccaattct tttatagttt ctattgattt tggatgacga
    29521 agtataactg cgtttgaacc acctactaaa acacttgctg cagttgaaac ttccattgct
    29581 atagttcttt cttctggaca accccaatct ggctcatctt ctattggagc tatagcttct
    29641 ttaacatgac ctacttcaaa agctactggt gttatgatag gcatttgtaa tgtcttatca
    29701 ttttgaccaa atgctgcaag tctaattcta tccatagtag atgcaacgta ctcatatcca
    29761 taaccaactg ctgaacatcc aacattcata actatatttt cacctttaac gcctagttga
    29821 gttaacaata cgtttaattg tttagctagg tttatatcaa cagaagattc agctcctact
    29881 ttatgtgcat aagccatacc agctgatgct cctactcctt tgtagttgtc ttctactgct
    29941 gacataaata agcagttatg tccatccaat gtttgagcta atttttcaaa taatttacca
    30001 tctttttcat gatttcctga accagctaca actaaaggta attttattgc ttcaattact
    30061 gccttagcta catcagcaca ttcatcaact gacttatcta atccatttgg gtcagaacca
    30121 tcaaatttta aacaaataaa atctgcttgt gtattagctt caacatattt agcccactct
    30181 actggacaat tagctacatc tttgtataat tccttatatg aatcagtcca gctttcagga
    30241 taaacgtctg atatttgtat acctattttt ggagaattac ctacttctcc atcaaaacta
    30301 taaaaaggta atacattttc cccacctaat ttaattgctt tttcccctat tcctacttca
    30361 acttctgata tttttccaga atatttttga gtagacattt taaatgccat atttttttcc
    30421 cccttaagcc tattttaatt ctaatttagc aaaaatttct ttcatagcca ctttagattt
    30481 tgaatcttca ggtaaattaa ctaaaggaat acctgtagaa tcatattcat atatcaattc
    30541 atccataggt acaacaccga ttagctctag gttatgcttt tcaatttctt ctttaacacc
    30601 atcattcata accccatttg gaactttatt tacaatcaaa agtattcttc caacacttaa
    30661 ctttagttcc tcagctaaat ctcttattct agcaacagct tgtatgctac gtcttgagca
    30721 atcactaacc aataaaagtg tatcaacatg tctagtgact tttctactta gatgttccat
    30781 accagcttcg ttgtccatga ctaagtattt gtaatgacca gatattttgt ttacttgttc
    30841 tctaagtatc ccatttacaa aacagtaaca tccttcacct tctgacctac ccattactaa
    30901 taagtcatat ccttcgcctt cttcgattat agaatttaat ctaaattgaa gatattgtgc
    30961 ctttgtcata ccaccaggga atgcattgcc taacttttct ctttgattta cttcttctct
    31021 tatttctcct attgttgctt caacttcgat acctaatact tcatttatgt tagcattggc
    31081 atcagcatct acaactaata ctggtccctt tttgtcctta accaaataat caatcaaaag
    31141 ccctgtgaga cttgttttac cagttccacc ttttcctgca acagctatat tgtatcccat
    31201 tttaagactt cccccttaaa tctagccaca gagccttatg tactctgtct gtctattata
    31261 atttaggtac agaatgaaca cattctccat gtacatcatg aagtgcttcc attagccctt
    31321 cagataaact tggatgtgga tgtattgcat cccctacttg ttctactgtt aatcctagat
    31381 gaacagcaag tgacaactct gtcaataaat ctgtagcatg aggtcctact actgctgcac
    31441 ctattatctt atcagtttct ttgtcagcta taacttttac aaatccttgg aaatgtccta
    31501 tagcttgtgc ttttccaagc cctctaaaat caaattgacc tacattgtat tctacacctt
    31561 cagcttcaag ttgcttctca gtcttaccaa caccagcaac ttcaggttca gtataaacac
    31621 atcttggaat tgctctataa tctactacct tagtcttacc taaagcattt tcaactgcta
    31681 cgataccttc tttagaagca acatgtgcta ggaaaggagt atctattata tcgcctattg
    31741 cgtatatacc ttctacatta gtttcaagat gttcattaac tactactttt cctctttcca
    31801 tttcaattcc tatatcttct actccagaat tgtcaaggtt aggtcttcta ccaacacata
    31861 ctaatgcata ttgtgcttct ataacctttc cattagaaag agttgcaaca gccttaccat
    31921 caactacttc acaagtttgt actccaatac ctgtaataac cttaatttta tctttcttaa
    31981 attgtctaag tagttgttta gctacatctt tgtcttcatt taatagtatt tggtcaacca
    32041 tctcaactat agttacttct gttcctaagg ctctaaagaa ctgccctatc tcacatccta
    32101 taactccacc acctactatt aacatagact caggtatctc ttcaagtcca agaacttcat
    32161 cactagttat tacaactttt ccatcatatg ggaacattct tggtacaact ggaacagaac
    32221 catttgctag tattatctta tctgctttta tagtttctac tgttccatca tctttagtta
    32281 cttctatagt gtttttatca attaattttc caaaaccatt aactaggttt actcctcttt
    32341 tttcaaataa gaactctata ccacttatta attggtttac aactttattt tttctttcca
    32401 ttatggcagt gaagtttggc ttaactgtac catctatttc tataccaaaa tcctttgctt
    32461 cttttacagt attcaatact cctgaagaag caagaagtgc tttagttggt atacatcctg
    32521 catttaagca agttcctcca actcttcttt tttcaacaac tgttacatct gcaccaagca
    32581 tagaagcttt tatagctgct acatatcctc ctggtccacc acctactact actatcttca
    32641 tctttaattc ccccaaacct aatataatct acaatcttgc aatactataa acctgcggca
    32701 tccagtatag caggtatatt ctcttctgcc ccaattgcca ttatatgaac accatcacat
    32761 aaatcttctt cttttaattg tttgataagt tcccctgcca tttttattcc ttcacttacc
    32821 cagttttctt ttccagctgc tcttaatctt tcgatttgtt catctggaac aaatattcct
    32881 ggtacatttg cagtcataaa tttagccatt cctggtgatt ttaaaggtac tattcctgct
    32941 aataccttgc aatccatatc ccttgttaat tctctaaact ttctcattgt atttatgtca
    33001 taaagtgctt gtgtttggaa aaatttagcc cctgctttta ttttcttttt catttttaat
    33061 aattgaactt ctattggaga atattctgga gttactgatg ctcctagata aaaatctgga
    33121 gaacctttta acttattacc agccatgtcc gtacctgcca ttaaagtctc agcagtttga
    33181 agtatcccca cagaatctaa atcaaacact cccttagctt gtggatggtc tcctacgcta
    33241 gtatggtctc ctgttagtgc caacagattg tttatcccaa atacacctgc tgacaacatt
    33301 tctccttgta tagctattct atttctatct ctccctgtta tttgaataac tggctctaat
    33361 ccagcatctt ttaataattt acatgtagca agagaagttg ctctcataac tgccgattga
    33421 aagtctgtta catttgctgc atgaactctt ccaactaacg gctttgcaca ttctattaaa
    33481 tgtgaaaggt cagtcccttt tggaggagcc atttcagtag taactgcaaa ttttccactt
    33541 tctaatgttt ctcttaataa gctcatctct aatccccctt ttaaccttct aaaaaaaact
    33601 tttatgaatt tgctgctgct tctttctttt tctttgcact taaacttctt ggattatttt
    33661 gcttagaata gtcttttggt ggtcttattt ctgctaagtt atctaattgt cctatagctt
    33721 ctagtctttc atatatcttt atccaagcac aatcattctc tgagtttact tcacacttac
    33781 catttttagc tccaccacaa gccccattca taagaccttt agcacacata gttactggac
    33841 atatcccacc tgtccaacca agttcacagt ctccacaagc cttacaggct tcttcatatt
    33901 ctccaactct ttgtacttcc cctataaaca aagtattgtt ggctggataa actggcttat
    33961 cttttaaatt cttaactatt gtctgtgtac catctccaca tgctaaagat aaaactgcat
    34021 cagcttcctt agtctcttct tttagagcct ttaaatcctt tttagacttt aaaaggttac
    34081 aagcagggtc aagcattaca tatcctaaga tttttttgcc ttcaccttca agagtttcct
    34141 tcatttttaa aacttcttct tccccaccac ttttacaagt tgctgcacat tgattacaac
    34201 caactaaaac cagcttgtca aaatccttta agtaccctag cacttcttct aatggtttat
    34261 tttcagaaat tatcatatct acttcccccc taaaccttcc acatatatat ctatttattg
    34321 ttttgtaatt tacaagcctt aactacatgt tttgcaagta tagatgttgt aactgaacca
    34381 acccccgctg gaactggagt tatcattgat actttgtcta aaactgcatt tgtatcaaca
    34441 tctccacata aatttccttc ttcatcaaca tttataccaa cgtctataac taccgctcca
    34501 tctttaacaa aactttcatc aaccatctta gctctaccaa tagctgctat tagcacgtct
    34561 gcctctgctg ctacacctga tagattttta gtttttgaat gacatattgt aacagttgca
    34621 tgttcactta ataaaagcat tgatactggc tttccaacta ccattgatct tccaagaact
    34681 gttaccttac ttccttttaa atcaacatta tagtgtttta atatctcaac tactgctgta
    34741 ggtgtacatg gtggaaatcc acttttatcc ccttccatta ccttagcaga gtttattgga
    34801 ctaaaacaat ctacgtcttt ttccggtgca ataacatatt ttataacttc ttcatttaag
    34861 tgttttggaa gaggtctaaa acataatata ccattaacat ttttgtcatc attaactctc
    34921 tttaatacat caatatattc ctcttgtgtt atatcctcag ctaactctaa cacttctgtt
    34981 gttataccta tgttttgaca tctctttagt gctcctcttt cataagataa atcatcactt
    35041 cttgccccaa ctctaactat tgttagttta ggatttatac cttctttaac tagtaaatcg
    35101 acttctttta ttaattcttc actaatctta tcagctacag gttttccttt tataatttgt
    35161 cctttagttg acattccttc catacattca caactcccct acaagcaaac ttaaattaat
    35221 ttattctata taccacatat taataacata gtatctactt tcgtcattta aaaattattt
    35281 tccaagagct ttttcaactt tggcataaac ttcatcacaa atttttacac catcttcaac
    35341 taacttatct acttctgttt ttaccttatt aacatattct ttatcttgta tagaatttat
    35401 atttataact acatttaatt gtccaccaag tatagcagct cttaaacact gaactcctac
    35461 accaacatca cttatagcaa gtcttgaacc tttatcaact aaatcttcat gtaacttaat
    35521 agcttcatag caaactctta ctatgtttaa tggtacttca caagcaactt ttaatgcctt
    35581 ttccattgtt tctgctttta ttcttttttc ttcttctgtt tctttaggaa gcccgtaagc
    35641 ttttgataat ggtaagaaac attctgcatc atcatctatc atttttaata aatctttttc
    35701 taactttcct gccttactta atatctcttt tacactttct tcatactcag catacttctt
    35761 cttccctatt gtaaggttac ataccatact acctaaagcc atacctattg cacctactag
    35821 tgcagcagct cccccaccac ctggtactgc ttctttggaa gatagtactt ctacaaaatc
    35881 aacacaagtt ttatctgcta ttttcatgat aatcctcctt gaaaggatgt aggaaaatat
    35941 ctcaaattgt tcgagatatt ttacgaacta ttttaagata tctcctactc tttatttata
    36001 aattaatttc cttagaataa tccagctatt actccatttt catcaacatc tattttttct
    36061 gctgctggaa cttttggtaa tcctggcatc ttcattattt ctccagtaag tgcaactata
    36121 aatccagctc ctgctgaaat tgttgcttgt cttacagtta ttctaaatcc tgttggtcta
    36181 cctaatttag tttggtcatc agttaaagaa tattgagtct tagccataca tactggaact
    36241 ttagtaaatc caagtttttc taagttagct atttccttat cagcttcagg agtataatct
    36301 acaccatctg ctccataaat cttagtagct atagcattta tcttgtcttt tatagataaa
    36361 tcatcttcat agcagaatct aaagttattt tcttcttcat ccaataatct taaaacttct
    36421 ttagctactg caattccacc ttctccacct tttgcccata cttcagaaag agcaacatta
    36481 acacctaatt ccttacactt gtcttcaaca agtttaagtt cagcttcagt atcaagtggg
    36541 aatctattga ttgcaacaac tgctggtaat ccatatactt gagttatgtt ttcaacatgt
    36601 tttaataagt ttggaagtcc tttttcaaga gcttctaggt tttcgttatt taagtctgct
    36661 ttagcaactc caccattgta ttttaatgct cttacagtag caactattat aacagcatct
    36721 ggttttaagt ttgccattct acattttata tctaagaatt tttctgctcc aaggtcagca
    36781 ccgaaaccag cctcagttat tacataatct gcaaagtgca tagccattct tgtagctata
    36841 acagagttac atccatgagc tatatttgcg aatggtccac cgtgtacaaa tgatggtgtt
    36901 ccttcaagag tttgaactag gtttggtttt aaagcatctt ttaataatgc agccatagca
    36961 ccattagctt ttaattgtct agctgttaca ggttcacctt cgtagctgta tccaactatt
    37021 atgtttccta atctttcttt taaatcagat atatcacttg ccaaacagaa tgctgccatt
    37081 atttcagaag caacagttat atcgaatcca tcttgtctaa ctgctccatc acctttttta
    37141 cccataccat caacgatatt tctaagttgt ctatcgttca tgtctacaca tcttctccaa
    37201 gttattttct tagggtctat tctaagtgca tttccttggt gaatatggtt atcaagcata
    37261 gcagctaaaa gattgttagc agctcctata gcgtgaaaat ctcctgtgaa gtgtaagttt
    37321 atatcttcca taggaacaac ttgtgcatat cctccaccag ctgctccacc tttaacacca
    37381 aatactggtc ctagagatgg ttctcttaaa gcaactaata catttttgtc tagtttagca
    37441 aatgcatcag caacacctat agtagtagta gtttttcctt cacctgctgg agttgggttt
    37501 atagctgtag ttagtattaa cttagctttt ttaccagtct ctctttttag tagattgtag
    37561 tcaacttttg ctttgtattt accataaagt tctacatcat cttcacctag tcctaatttt
    37621 tttgcaacct ctctaatatc ttgaggctta gcttcttgag ctatttcaat gtcagattta
    37681 aatcccataa ctgaatctcc tcccacaacc taaatagatt taaaatataa tttttgtaaa
    37741 aattgtatta atatgtaaaa attatatttt ataagataat tatacatgta aaattactta
    37801 aaaactagct actaagctat taaaatattc aaacttaata gctagttatt tatctaatta
    37861 cttacttatt acctatctat tgcctatgtc taacctttaa ccaacttaaa atcccaaatc
    37921 aaaatttcta tctattttat ttatccttaa gtgacattaa tttatctttt attgctttaa
    37981 tctgttcttt cgttgtttca ctagaatcat atggagattg attagctcta tcagaatcaa
    38041 taacatcttg gttataataa ataaatccta aagattctcc atcttctaag ttttggatta
    38101 taaattcttc atcttcctta tttctaattt tatttcctac tacaaatact ttatttacac
    38161 ctatatcatg tccaagtttc ttaacttttc tataagtctg tagacttctc tctcctggtt
    38221 ctacaactac tataaaagca tctacacctt gggctgttcc tcttcctaag tgttcaattc
    38281 ccgcttccat atccataacc actacatctt tattttgtag tattaaatgt gagcagagtc
    38341 tcttcaaaag aacatgctct ggacatacac atccagtacc accagaatct acggttccca
    38401 atgtaagtaa tcttactcca ttgtattctt tacagaaatt ttcagggata tcgtctactt
    38461 taggattcat cttaaacatt tttccaaatg aacctacaga agcagctgtt ctatctgaaa
    38521 ctaatttttt catttctgat ataggtacta ttgattcata tacttctttt ggaaaaccta
    38581 gtgctaaagc taaattagca tcagggtcgg catcaacagc aacaactctg tatccgtctt
    38641 cagcaaacat cctagagagc attgaagaaa aagtcgtttt acctactcca cctttacctg
    38701 ttattgctat cttcataatc cctaccacct tttattacta tttgcttttt agtagttaat
    38761 tttacagtta aatatttttt atactctata tttttataca ctaaaaataa actacagcta
    38821 gtccttacag gaaccaatta aatttaccct actagctttt caaagcagca tgtatagttt
    38881 tattattctt taatattgtt tatattttat ttattcagta tattcattat attttgatgt
    38941 tctttacttt aatatttgac tttattcaac tttattcaac cagaaacttc ttataaaaac
    39001 gtttattcta taacttcact attttttaag cttcagcctt ttctgtcatt ttttcttcaa
    39061 ctaatgcttc aaagtgaaca cgttttttct ctatatcttc tattattgta gctgctaact
    39121 cttttggatt tgtatttaca gtaaatgtag ctccaacttt atcttttaat tcactagtta
    39181 atatatccac tgctcttgaa gaaccagcta ttggaggcat tattcctaaa taagtgtcta
    39241 taccagaagc tacaacatag catcctatag caactgcttt ttctgacatc cactctggag
    39301 ctatacctac aactggaagg tcacacatat ccatatctaa gtaattagct gctgctccaa
    39361 ctaaatctag tatacggctt atatcaacac aagaacccat atgtagtact ggtgggattc
    39421 ctactaattt acagacagtt gcaagacctt tacctgcata tttttcagcc gcatcagatt
    39481 ccattaaacc aaacttagct gctgctgaag ctccacaacc tgtagtaact actattatgt
    39541 cgtttttgat taattctttc attattgtta cgtgagcctc atttgaaact cctttagcat
    39601 tgttacatcc aactacacca gctgctcctc ttaatactcc agcttttaga cagtctgtta
    39661 atggcttaac tgttcctgct gggtctatat gagagtttac aactctgtct aattgattta
    39721 ctactgcttc tacactatac ccaactgttg cccctgattt taattcaggt ataagtactt
    39781 ttgatttatc tctattttta aagtttaaaa tagcttcttt aactattgct ttagcatcat
    39841 caagtgcttg ttcttctcta aattcaatat aagttgaacc tgttatctta gcttttggag
    39901 aagttgttac aaatttagta tggtaacaat ctgcaactct agctaatgca gggaatatac
    39961 attgaacgtc aactattaca gcttcaactg ctccagttat tattgcaagt tcttgttgat
    40021 ggaagtctcc tgctattttt acaccatgtc tcattgtaat ctcattacct gtacaacaca
    40081 tacctgctaa gtttattcca tcagcaccaa cttctttagc taaagctact aagtctggct
    40141 cttctgatgc taaaactatc atttctgata atgctggctc atgtccatgt aatattatgt
    40201 taactttatt ttcttctaat actgctaaat tcgcctctgt tcttcttggc actggagttc
    40261 caaataagat atcgcttaga cgagtaccta tcatagaacc accccaacca tcagccatag
    40321 aagttctaag actcatgtgt attaaactat ctatatcacc agtacatcct atatgagtag
    40381 agtgcattat tgttgcaatt tctctatcta tagctcttgg ctctatagcg tattctttcc
    40441 atattttttg tcttggttct ggtgcatttt ttataaatct taatgtccca aaaggttttc
    40501 caaattccat taatgcaatt tctgccactt catgtgctac atcatagata tctctacctt
    40561 ctgtctctac atcccattct ttagctaaag taattaactt agcttcgtct cttattttat
    40621 agtttccatt tggatcagct aatgctaatg tatgtgctat atctctagca tggtctgaat
    40681 gggcagctgt cccaccagca accattcttg caaagtttct acctactata gtatgctcat
    40741 ctgctccaca taaccccctt ggagtcttag ggcttattct acatggtccc attccacata
    40801 ttctacagca aacaccttga agaccaaaac cacattgtgc cttaaagcct tcttttctat
    40861 catacattgt ttctacgcct tcttctctag ctttagctat catcttctgg ctgtttaaat
    40921 caattgttaa cattttttca tccatattcc tatccccttt ttttgatttt taaaatcccc
    40981 caggtgactg acttcatgtc ataaaataat aaaataaatg ctttctaagt tgattatata
    41041 ctaattttca gtgctttgct ttagatttaa atattttttt aacaaaatta gataatacat
    41101 aaaaatttta ttttctacac ttaatttaag ttgattttat cgcttgtttt tggctatttg
    41161 ttaatatcta aacctacttt tttgatactt cagtttaatt aaattatgtt ttgatttttt
    41221 taaatcaaat ttgaaatttt tctagtcatt tttatattat tgcagattgt ttgtgtattt
    41281 attatctttc actactgttt ggacttatta cagattttta ctccctaaac ttaattagta
    41341 aaaacttctt agaatagcat tttttggact aatttttttg aattttttta tttttcctac
    41401 tttaatatta attatagctt caaattgaac atttgtatat attcatttga agctataatt
    41461 attttttata taaaactttt ttaatacagc attaatttag acattttgac tattttttct
    41521 actttaattc atttatttca agcgatttat cctatttcat atttttagct acaatagccc
    41581 catatgcaat agaatataat ttagattcat gtgaatcagc tcttgacact aaaactatag
    41641 gagcttttgc acccataatt atacctgctg atttagagtc tgccatatat gtaaaggctt
    41701 tccctattcc attaccaacc tctattgttg gaaccaatat gatatctaca tctccagata
    41761 cttcactttt aaaccctttt atctcactag cttcttttga aacagctaaa tcaaaagcca
    41821 gaggaccttc tacaattaca ttttcaccaa attctccttc tttacaagct tttgctaaca
    41881 tatctgcatc tacagttgct tgcatcttag gatttacttt ctctttagct gccaaacatg
    41941 caacttttac agtttccatt cctaaagctt ttgcagcttt tatagaattt tttaatatct
    42001 ttgccttttg ttcataatca ggagctatat tcatacctcc atctgtagtt ataagtaatt
    42061 tatggtattt ttctaactca tataccataa catgacttaa taaactatca gttctaagac
    42121 catagtcttt atttaacact tcttttaata tgacagatgt atctaataat cctttcatta
    42181 caaagtcagc ttctttactt gatactaatt taactgctat ttctgcacac tctttgatat
    42241 catccttatc cactattttg ataccactta aatcaaatcc tatttctttt gatatttctc
    42301 ttattttgcc ttcatttcca accaatatag gagtaattat atttctttct acagcatcct
    42361 ttattgccat taaaacttct ttatcatgag ctgctgctac agagagtaca attttctctc
    42421 ctcccttaag ttgttctaaa atatcatcta atttttttat caaaagtaac ccctccctta
    42481 tatatattta actaaataat accatatttg ctataccttt tctcaattta ttttttatgt
    42541 tataaagaat ttatataact aaaagacagt ttaatatttt attctgggat ttcatcaata
    42601 actcttaaaa gctttgcagg aacaccacct acaatagtat tttctgcaac attttttgtt
    42661 acaactgctc cagcagcgat aattacatta tctccaatag taactcctgg aactatagtt
    42721 acattagcac caatccatac attttttcct attgtgattg gagaaggata agttgtacca
    42781 cgtttctctg gtgcaatacc atggttaagt gtagccaata ccacattatg cccaatttgc
    42841 gtattatctc caatatcaat ccccccctgg tcttgaaaat ggcaactgct atttataaaa
    42901 acattttttc ctattgtgat gttttttcca caatctgtat aaaatggtgg aaacataaaa
    42961 aatgattcat ctaccttttt tccaattaac tcagaaaata aactacggac ttcttctggt
    43021 tcatgataag aagaatttaa cttacttgtg attttcattg cttcattact caacttaacc
    43081 ataaattcat gtatttctga tttactagaa acaggttttc tttgattgat atgtgttaaa
    43141 aattcattga tattcatttt atacatcctt tcaatatgtg ctttaattta tttgtacatt
    43201 ctaaataata ttttaataaa aattaattta a
//
LOCUS       DAEOSP010000012        43055 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.012, whole genome shotgun sequence.
ACCESSION   DAEOSP010000012 DAEOSP010000000
VERSION     DAEOSP010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43055
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.012"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            6..82
                     /locus_tag="KRH54_000490"
     tRNA            6..82
                     /locus_tag="KRH54_000490"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40..42,aa:His,seq:gtg)
     gene            91..166
                     /locus_tag="KRH54_000491"
     tRNA            91..166
                     /locus_tag="KRH54_000491"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:124..126,aa:Lys,seq:ttt)
     gene            174..247
                     /locus_tag="KRH54_000492"
     tRNA            174..247
                     /locus_tag="KRH54_000492"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Cys,seq:gca)
     gene            255..331
                     /locus_tag="KRH54_000493"
     tRNA            255..331
                     /locus_tag="KRH54_000493"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:289..291,aa:Arg,seq:acg)
     gene            344..419
                     /locus_tag="KRH54_000494"
     tRNA            344..419
                     /locus_tag="KRH54_000494"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:377..379,aa:Val,seq:tac)
     gene            495..908
                     /locus_tag="KRH54_000495"
     CDS             495..908
                     /locus_tag="KRH54_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578168.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            1216..4647
                     /locus_tag="KRH54_000496"
     CDS             1216..4647
                     /locus_tag="KRH54_000496"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBG8578169.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            4889..6817
                     /locus_tag="KRH54_000497"
     CDS             4889..6817
                     /locus_tag="KRH54_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG8578170.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
     gene            7270..7734
                     /locus_tag="KRH54_000498"
     CDS             7270..7734
                     /locus_tag="KRH54_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBG8578171.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            7741..8235
                     /locus_tag="KRH54_000499"
     CDS             7741..8235
                     /locus_tag="KRH54_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBG8578172.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            8251..9276
                     /locus_tag="KRH54_000500"
     CDS             8251..9276
                     /locus_tag="KRH54_000500"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBG8578173.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            9263..11710
                     /locus_tag="KRH54_000501"
     CDS             9263..11710
                     /locus_tag="KRH54_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBG8578174.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            11890..13263
                     /gene="radA"
                     /locus_tag="KRH54_000502"
     CDS             11890..13263
                     /gene="radA"
                     /locus_tag="KRH54_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBG8578175.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            13266..14336
                     /gene="disA"
                     /locus_tag="KRH54_000503"
     CDS             13266..14336
                     /gene="disA"
                     /locus_tag="KRH54_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBG8578176.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            14425..15519
                     /locus_tag="KRH54_000504"
     CDS             14425..15519
                     /locus_tag="KRH54_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBG8578177.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            15648..17825
                     /locus_tag="KRH54_000505"
     CDS             15648..17825
                     /locus_tag="KRH54_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578178.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDSIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(18011..18856)
                     /locus_tag="KRH54_000506"
     CDS             complement(18011..18856)
                     /locus_tag="KRH54_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8578179.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            19073..20239
                     /locus_tag="KRH54_000507"
     CDS             19073..20239
                     /locus_tag="KRH54_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBG8578180.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            20261..21907
                     /locus_tag="KRH54_000508"
     CDS             20261..21907
                     /locus_tag="KRH54_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBG8578181.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            22004..23377
                     /locus_tag="KRH54_000509"
     CDS             22004..23377
                     /locus_tag="KRH54_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8578182.1"
                     /translation="MNTEVNVKATTIKYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            23822..24775
                     /locus_tag="KRH54_000510"
     CDS             23822..24775
                     /locus_tag="KRH54_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBG8578183.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            24813..25775
                     /locus_tag="KRH54_000511"
     CDS             24813..25775
                     /locus_tag="KRH54_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBG8578184.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            25800..26786
                     /locus_tag="KRH54_000512"
     CDS             25800..26786
                     /locus_tag="KRH54_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBG8578185.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            26799..27845
                     /locus_tag="KRH54_000513"
     CDS             26799..27845
                     /locus_tag="KRH54_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBG8578186.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTAKETIVSTNTSKSSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            28012..29742
                     /gene="lpdA"
                     /locus_tag="KRH54_000514"
     CDS             28012..29742
                     /gene="lpdA"
                     /locus_tag="KRH54_000514"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBG8578187.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDTV
                     KENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSMPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVAAVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            30160..33108
                     /locus_tag="KRH54_000515"
     CDS             30160..33108
                     /locus_tag="KRH54_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8578188.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEVISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPVEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            33086..33550
                     /locus_tag="KRH54_000516"
     CDS             33086..33550
                     /locus_tag="KRH54_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8578189.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            33663..33965
                     /locus_tag="KRH54_000517"
     CDS             33663..33965
                     /locus_tag="KRH54_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8578190.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            34027..35391
                     /locus_tag="KRH54_000518"
     CDS             34027..35391
                     /locus_tag="KRH54_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG8578191.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            35426..35899
                     /locus_tag="KRH54_000519"
     CDS             35426..35899
                     /locus_tag="KRH54_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578192.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYSIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYELKDIEKIRIREFYNKKI
                     YLKINDGNLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            36071..36721
                     /locus_tag="KRH54_000520"
     CDS             36071..36721
                     /locus_tag="KRH54_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG8578193.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            36895..37827
                     /locus_tag="KRH54_000521"
     CDS             36895..37827
                     /locus_tag="KRH54_000521"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG8578194.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            38101..38814
                     /gene="ispD"
                     /locus_tag="KRH54_000522"
     CDS             38101..38814
                     /gene="ispD"
                     /locus_tag="KRH54_000522"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBG8578195.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVKETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            38811..39296
                     /locus_tag="KRH54_000523"
     CDS             38811..39296
                     /locus_tag="KRH54_000523"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBG8578196.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    39391..39599
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            39663..41378
                     /locus_tag="KRH54_000524"
     CDS             39663..41378
                     /locus_tag="KRH54_000524"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG8578197.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFNRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            41500..42945
                     /locus_tag="KRH54_000525"
     CDS             41500..42945
                     /locus_tag="KRH54_000525"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG8578198.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
BASE COUNT        17576 a         4551 c         8063 g        12865 t
ORIGIN      
        1 ttatggtggg tatagctcag ttggttagag cgccagattg tggctctgga ggtcgtgagt
       61 tcgactctca ttatccaccc caacttatat ggtctattag ctcagtcggt agagcacctg
      121 acttttaatc agggtgtccc gcgttcgagt cgcggataga tcaccaaaaa ggcggcgaca
      181 tagccaagtg gtaaggcagt ggactgcaac tccttgatcc ccagttcgaa tctgggtgtc
      241 gcctccaata aaatgcgctc atagctcaac tggatagagt gtctgactac gaatcagaag
      301 gttaggggtt cgagtccctt tgggcgcacc atatgaatag cgagggatta tagctcagct
      361 gggagagcac ctgccttaca agcagggggt cacaggttcg agccctgtta atcccaccag
      421 tgcaaacctc tagtaaaact actagaggtt ttatttttga ttgaaaaagt ttttatattt
      481 aggaggtaaa aaatttgtat atagtaatag gaaatgaaat agttgatagt gaagaactta
      541 aaataattat agataaaaat agtgaattta aagttgaaaa agatttaagt aaaagcacaa
      601 aaagagaaga tgtaatagca tatcagttaa gtatagattt aaattaccta gattctctta
      661 taaatgagca atgtaattta gccagtctat ctgatgaaga gaaatttgat gaatatatga
      721 ctttgtctga tgaacttgca ctggatttag aagagttaat gccaaaatat acaataataa
      781 atgctagagc atataaatta gacgaggtag atggaattgt aaaaataata ctagctgtag
      841 cgtatgcaga tttagggcac ttaaaacttt ctgatgtagt aaagaggcta tctagacaag
      901 tagattagga tatgaatatg aactatatta aaaaaatgta taaaatctta cgtaaagatg
      961 aaaataatta tgtaaacata gaaaaaacag tataaaatca agtcaattgt agaaattttc
     1021 acataaattc atctgaaatt ctataaaaaa tgccttaatt ttaatgaggt aaatcgagtt
     1081 tacacagtct aaaaattact tatatttata aatttatctt attaattaag gaactttctt
     1141 aaacatcttc aaaaataaag tattttagtc tataatcttt aatattacac aaacaataaa
     1201 ggagaagaat agagtatgct taaaaagttt aataaaatac tagtggctaa tagaggcgaa
     1261 atagctataa gaatatttag agcctgttca gaattgggga taaaaagtgt tggtatatac
     1321 agtaaggaag ataagtatgg attgtttaga acaaaagcgg atgaatcata tttgatagga
     1381 gaaggtaaag ggccaataga tgcatattta gatatggatg gaataataga cctagcaaag
     1441 agaaaaaaag ttgatgctat acatccagga tatggttttc tagcggagaa tgcagaattt
     1501 gctcgtaaat gtgaagagaa tggaattact tttataggac catcatctaa ggtaatgaat
     1561 atgatggggg ataagattaa ctctaaaaaa atcgcaaaag aagttaatgt tcaaactata
     1621 ccaggagtag aaaaagcaat aagaagtact gaagaagcaa aggaagttgc aaacaaaata
     1681 ggatatcctg ttatgataaa agcatctaat ggcggtggcg gtagaggtat gagaattgta
     1741 catcgcgaag aagacttaga gcttgaatat gaaactgctt gtagtgaatc aagaaaagct
     1801 tttggagaag atattatatt tatagaaaaa tatatagctg atccaaaaca tatagaagtt
     1861 caaatattag gtgataatta tggaaatata gttcacttat atgaaagaga ttgttcagtt
     1921 caaagaagac atcaaaaaat aatagaatat gcaccagctt tttcgttaga tgataaagta
     1981 agaaaagaga tatgtgaaga tgcagttaag ttatcaaaac atgtgggtta ttctaatgcg
     2041 ggaacattag aatttttagt agatgcaaat ggtgggcatt attttataga gatgaacaca
     2101 agagtacagg ttgaacatac tgtaacagag atggttacag gaatagatat agttcaaagt
     2161 caaatactaa tagcccaagg ttacagctta gatagtgaag aaattaatat aaaatcacaa
     2221 gatgatgtgg aaataagagg ttactctatc caatgtagaa taactacaga agaccctaag
     2281 aataaattca tgcctgatac aggtaaaatt caagtttata gaactggttc aggatttggt
     2341 ataagactag atggaggaaa tggatttaca ggggcaaaca taagtcctca ttatgatagt
     2401 ttattggtta agacaatatc ttgggataga acattccaag gagcaataaa taaaactata
     2461 agatctataa aagaacttag agttagaggt gttaaaacta atgtaggatt cctagttaat
     2521 gttttaaata atcctatatt ctcaaatggt aaatgtagta ctaagtttat agatgaaaat
     2581 ccagatttat ttgaaataac agaaagtaaa gacagaggaa caaaattact tcaatttata
     2641 ggagatgtaa ttgtaaatga taatgcttgt aaagaaaaac cattatttga tgcattacat
     2701 gacccaagaa tggataagga tggttcaaaa tcagaaggaa gtaaaatact tttcgataag
     2761 ttaggtaaat ctgcatatat agaaaagata aaaaatgata agaaattact attaacagat
     2821 acaacaatga gagatgcaca tcaatcatta ttagcaacaa gaataagaac atatgactta
     2881 ttaaaagctg ctaagccaac agaaaaatat caaaaggatt tattctcact tgaaatgtgg
     2941 ggaggagcta cttatgatgt tgcatataga tttttaaaag aatctccttg gcgtagatta
     3001 caaaagctaa gagaagaaat accaagcata atgttccaaa tgttgcttag agcatcaaat
     3061 ggagttggat ataaaaacta tccagataat gtaatagaag aatttacaaa agaatctgct
     3121 agacaaggta tagatgtatt tagaatattt gattcattaa actgggttga aaatatgaaa
     3181 ccatctatta atacagcttt agaaactgga aaaattgttg aagcaactat gtgttataca
     3241 ggtgatatac tagataagac taaaactaaa tacaatctag aatattatat aaaaatggca
     3301 caagaattag aaagcttggg agcagacata atagcaataa aagatatgtc aggtcttctt
     3361 aaaccatatt cagcttacac attagttaaa gagttaaaga aaaatgtaaa agcaccaata
     3421 catttacaca ctcatgatac aagtggaaat ggtgtagcaa cttgtttaat ggcgtcagaa
     3481 gctggagtag acataattga tgctgcatta gaatctatgg caggacttac aagtcagcca
     3541 tcacttaatg ctatagtaga ggcacttaag aatacagaga gagatacagg aatagattta
     3601 tttggatatg atgagttagg aaaatattat aaggatttaa gaaaagttta taataaattt
     3661 gaaagtgatt taactaattc ttgtgcagaa atatataatt ttgaaatacc aggaggccaa
     3721 tatacaaact taaaacctca agctgatagt ttaggattag ttaatagatt tgatgaagtt
     3781 aaagaaaaat ataaagaagc taatgaagtt gtaggagata taataaaagt aactccatct
     3841 tcaaaagttg taggagactt agctatcttt atgactaaaa ataaattaga taaagacaat
     3901 ataatagaag aaggtaaaaa tttatcattc ccggattctg ttgtggatta ctgtaaaggt
     3961 atgattggtc aaccagaagg tggaatacca aaagatttac aagaggtagt tctaaaaggt
     4021 gaagaagcta taacagtaag accaggttca ttattaccag cagaagattt tgatgaaata
     4081 gctaaatatc taaatgaaaa gtatgatata aatgctaata taagaaatgt tataagttat
     4141 gctttatatc caaaagtata tgaagattat atcaaacatc tacaacatta taatgatata
     4201 tctaaacttg aaagtgatgt attcttctat ggattaaata aaaatgaaga atgtgaagta
     4261 gaaatagaag aaggtaaggt attaacaata agattagttg aaattggtga agttaaggaa
     4321 aatggattta gaactatagg atttgagtta aatggaatgg ttagagaagt agaaataaaa
     4381 gataagaatt tctctggaaa aattaataat gttgaaaaag ctgatatgaa tgaccctctt
     4441 caaattggag caagcatacc aggaaaagtt ataaaaataa tggttaaaga agaagatgaa
     4501 gtaaaagcaa atcaaccatt aatcgttata gaggctatga agatggaaac tataatagtt
     4561 gctaagactg atggagtaat taagtcaatc aaagtaaaag aagatgatat ggttgaagat
     4621 aaacaattat taatgataat gaagtaattt aattaaaata aattaaaaag cactctgaaa
     4681 tgtttaataa aaaatgtttt agagtgtttt tatttatttt aattaaatat ttgttaaaaa
     4741 aaagaggaca attacctcca gatgtagaaa taaagctagt aaggaaaatg ttatctaaaa
     4801 attctaaata tagtaaatat tttccataat atttcttagg aaataacagc aaagataaaa
     4861 tacataaatt taaaaggtgg gtgcttatat gaaggtttat gatagtaaaa tgttgagaaa
     4921 tgtagcggta ttaggacata gcggatgtgg aaaaacaaat ctaatcgaaa ctatagctta
     4981 cacagcaaac actaataaga tacctaaatt aacagacaag gtaaatatga cttatagcat
     5041 gggattaata cctatagagt ataatgatta taagtttaac ttattggata ccccaggata
     5101 ttttgacttt agcggagacg ttgtgtcatc tcttagagca agtgatgcag caataatagt
     5161 aatagatgca acagcgccta ttcaagttgg tacagagaaa tcattagaac taactgagag
     5221 tattcctaag ataatgttta taaataaaat agataatgaa aaggcaagat acaaagatgc
     5281 aatagcaatg ttaagagaaa aatacaataa taagatagtt cctatgataa gtcctatata
     5341 caaagataaa aactttgtaa agttacataa tgtttttgaa aacatagatg atttagaagg
     5401 tgaatttaag gaacaagcta tgagtgttaa agaagcatta atggaactta tagcagaaac
     5461 tgatgaccaa atattagata aatattttaa tggagaagaa ctaactacag aagagataca
     5521 aaaaggtatt attataggta tacaaagagg tgatattata cctgtaatat gtggttcaac
     5581 tataaataat ataggcacaa aggaaatatt agatacaata tcaagttatt tagaacctat
     5641 ctttacagag gaaagcaaac catttagggg attagtattt aaaactatgg ttgacccatt
     5701 tgttggaaag atgtcatata taaaaattac tgagggtgta ctttctaagg ataaagatgt
     5761 ttttaatata aataaaaatg taaaagaaaa aatagctaat atatatacat tgagaaatag
     5821 tgaattagta gaaatagaaa aagctaaagc tggtgatata gtagttataa ctaaagttaa
     5881 ttctctaaaa acaggagaca ctatatctgc agataaagat gcagaagctt tagaaaagat
     5941 agattttcct aaaccacaaa tatattatgc agtaactcct aagaataaag gtgatgaaga
     6001 aaaagtagca tctgtattaa ataaactagt agaagaggac cctacattac attggtatag
     6061 aaatacagag acaaaacaag cacttttagg aggtcaagga gaacttcata taaaaacaat
     6121 taaaaataaa atgaaagata agtttggtgt tgatgttgaa ttgaatgacc ttaaagttcc
     6181 ttatagagaa actataaaag gaacagctga tgtacaagga aaacataaaa agcaatcagg
     6241 tggtcatggt caatatggag acgtaaaaat aagatttgaa agatgtgaat ctgattttga
     6301 atttacagaa gaaatatttg gtggttctgt accaaaacaa tatataccag cagttgaaaa
     6361 aggtttaaaa gactctatgc aaaaaggtat attagcagga tatcctgtta caaacataaa
     6421 agctacgctt tatgatggtt cataccatga tgtagactct tctgaaatgg cctttaagat
     6481 ggcagcaagt gcagccttta aaaaaggtat ggaagaagca cacccaatac tactagaacc
     6541 tataatgaaa cttaagataa ctgttccaga agaatatatg ggagatgtca tgggtgatat
     6601 aaataagaga agaggaaaaa tatttggaat ggaaccagat gacaaaggga aacaaataat
     6661 atttgcagaa gctccacaag cagagacatt taaatatgca atagatttaa gagcaatgac
     6721 acaaggtaga ggatactttg aaatggaatt agagagatat ggagaggttc catcacagtt
     6781 tgcagaaaaa ataattgggt tagctactgc aaagtaatga acaaaataaa tagaaaaata
     6841 attaatatat attattaaaa atatttttag ggttttaact aggtataggt ttatgttagg
     6901 taaacttata cctagttttt atacattttt gatgaataaa aaaatatata atactttctt
     6961 aaatatttta ataaaaagaa agtatttttt tattattatt tttacaaatg tttagtaata
     7021 aagatgagaa ggtatattta aaatataaag tatttaaagt tctgaaatct aatcatgaag
     7081 caaaaaatta gcatttggtt atcaataaat ataataaata aaaactcata attaatgaaa
     7141 gaataaattc atatctaaat ttctataaaa aatttaaatt tatacaattt agggctttaa
     7201 aaatttaaat aataggtgta aaataatagt caaagaaagt caaaaataaa aaggtgtgtg
     7261 gtgatattaa tggcaactat gacagatata attgaaaaat ttattaaaga tttgatggaa
     7321 gaagataata gcatacaaat tcaaagaaat gaactagcaa acttgtttag ttgtgcacca
     7381 tcacaaataa attatgtctt aacaacaaga tttacaatag atagaggata ttacattgaa
     7441 agtaaaaaag gcggcggagg atatgttcaa atagaaaaaa tacgaaaaag taaagatggg
     7501 catattagag aactactaaa tgaaaaaata ggaagtcaga tatcctataa aaaagcaaaa
     7561 gaattattag aaggtttaaa agagtcggat ttgattaacg aaagagaatt aaaattaata
     7621 ttatatgcaa tagatgataa atcattgtgt atgccaatat atgaattaaa agagaaggta
     7681 aggtctaata ttttaaagaa tataataata ggattattta gtatagagga gtgataattt
     7741 atgctatgtc aaaaatgcaa taagaataaa gcatctgtat attataataa gatagtcaat
     7801 ggagaaaaaa cagagatgta tttatgtagt gagtgtgcaa aagagaatac agaaatgaat
     7861 tttaacttag atatgccttt ttcaatgatg gatatatttt ctaatttagg ttttcaacct
     7921 aaaaaagaac ttgaggaaaa gctagtttgt cctaaatgca atacaacata tagtgagttt
     7981 aagaataatg ggagatttgg atgtagtgaa tgttataatg catttagtag tcaagtaaat
     8041 ccaatgcttc aaaatataca tgggcatata gaacatacag gaaaagcacc taaaaaatct
     8101 ttttataaga taagtgtaga aaatgagatt aaagaactaa aagaagattt agatagagct
     8161 ataaaaaatg aagagtatga gttagcagct caatttagag ataaaattaa atatttaaaa
     8221 ggttctattg actaagattg gagtgatatt atgaaagaga acatagtcat gaaatcaaga
     8281 gttaggctag ctagaaattt aaataactat ccatttccaa ataaattaga taaagaatgt
     8341 gccatggaaa taatagaaaa agtaaaaaat gcgtttatta attctaactt agaacaaaaa
     8401 gaagaatttg atttctataa aattgaagat ttagaccaat ctaaaaaaat gcttatggtt
     8461 gaagaacata taataagccc agatttagca gaaaatgata aaagtgcagt tatagtaaaa
     8521 aaagataaaa ctataagtat aatgataaat gaagaagacc atataaggat acaaactata
     8581 tgtgatgatt taaacttaga atatgcatat agtgtagcaa atgaaataga tgatttgtta
     8641 gaatcaagtt tagaatatgc ctttaacaca aagctaggtt atctaacatc ttgtccaact
     8701 aatacaggaa caggaatgag agcttctgta atgatgcatt taccagcatt aagtcaacta
     8761 ggctatatgg atgaacttta taaaatttca tcacaaatag gcatagcaat tagagggata
     8821 tatggagaac gaacagaagc attgggaaat atatatcaaa tatctaatca acttacactt
     8881 ggaagaacag agtctaatat tatagaaaat gtaagtggac ttacaaaaga tgcaatttca
     8941 aaagaaataa aagctagaga aatattgcaa aaaaaattag gtataaaact agaagataaa
     9001 atatttagat cgatagggac acttgaaaat tcaagagtaa tgagtagcgc agaggcaatg
     9061 agccatctat caaatataaa aatgggaata gaaatgaact atatagataa actagattta
     9121 agggctatag aacaacttat gataggtata caaccagcgc atcagtcaat aatgtataag
     9181 agtgatgatg ttgagaatag agatataaat agagcaacat atattagaga aactttggaa
     9241 aaattaagag gagggatgaa ttgtgaactt caatagattt acacaaagag ctaaaaaggc
     9301 aattgattta gcgtttgaat ctgctaaaag tctaggacac aatattgtcg gaagtgaaca
     9361 tatactttta ggtcttttaa gagaagaaga aggtatagct gctaaagttc taagtaaagt
     9421 tggatttaca gaagcttatt tagaaggcaa aatagttgat atggaaggta agggagaaga
     9481 aatctcagaa gatatagtat taagtccaag gagtaaacaa atacttgaat tatcaggaat
     9541 gtttgcaaat aaattaaaaa caaattatat tgggactgag catatattat tagcaattat
     9601 tcaagaaggt gaaggaatag ctaataagat tttaaattat gctggagtaa atgataggac
     9661 tttagcccaa ttaacaattg atatgatggg tattagtgat aaaaatcaat acaaagcaga
     9721 gaatagttac acgggcaacc agaatcaagc agaatctaaa gtacttgata agtatggaag
     9781 aaatcttaca ctatatgcaa aacagaataa aatagaccct gtaataggta gagaaaaaga
     9841 aatacaaaga gtaatacaaa tattaagtag aagaactaaa aacaatccag tgttgatagg
     9901 agacccaggt gtagggaaaa cagccatagc tgaaggtctg gctacaaata tagccttagg
     9961 aaatgttcca gaaacactaa aaaacaaaac tttatactct ttagagatgg gttcattatt
    10021 ggctggagct aagtatagag gagaatttga agaaagaatt aaagaagttg tagatgaagt
    10081 tgttaaaaat ggaaacataa tcttatttat agatgagatg cacacaataa taggggctgg
    10141 gtctactgga gaaggctcta tagatgcttc aaatatatta aagccagcct tagcaagagg
    10201 agaaatacaa gttataggtg caactacaat tgatgaatat agaaagcatg ttgaaaaaga
    10261 ttctgctcta gaaagaagat tccaaccagt tatggtagat gaaccaacta aagaggactc
    10321 aataaaaata ttagaaggat taagagataa atatgaagct catcataaag ttaaaataac
    10381 tgatgatgct ataaaggcag ctgtagaatt atcaactaga tatatatcag acagatatct
    10441 gccagataaa gctatagact tgattgatga agctgcatca aaagtaagat taaaagaaaa
    10501 tacacctcct tcagaaataa aaaaattaga actagaaata gaaaatatag ataaagaaaa
    10561 agaagaagca gtaagatgtc aagactttga gaaagcagcc aaaataagag atgaacaagg
    10621 tctacttaaa aaacaattag aagatgttag agaaagatgg aacaagtcat ctaaacattc
    10681 agatttagtt gatggagaag ttattgctga agtagtgggc ttatggacag gaataccagt
    10741 taataaaatt cttgaagaag aggctgatag acttttgaga cttgaggaaa tactgcacaa
    10801 tagagttata ggtcaagaac aagcagtaaa atctatttca aaggcaatca gaaggtcaag
    10861 agcaggtctt aaagacccta atagaccaat tggttcattt ttatttttag gtccaacagg
    10921 agtaggtaaa acagaattat ctaaggcatt agcagaagtg caatttggag atgaaaatca
    10981 aataattaga atagatatgt ctgaatatat ggaaaaacat gctgtgtcaa gaatgatagg
    11041 ttcacctcca ggttatgtag gtcatgatga aggtggtcaa ttaactgaaa aagtaagaag
    11101 aaatccatat tcagttatct tatttgatga gatagaaaaa gctcatccag atgtatttaa
    11161 tattctgtta caaattctag atgatggtag actaactgat tcaaaaggaa gaactgtaga
    11221 ttttaagaat actattgtaa taatgacatc aaatgttgga gcatctacaa ttggtagaca
    11281 aaaaacttta ggatttagta tagctaaagg agacgaagaa gaaaaatctc aatatgaaaa
    11341 aatgaaagaa aatataatgg gtgaattaaa acaaagattc agaccagagt ttttaaacag
    11401 aatagatgat ataattgttt tccactcact aaatgaaaat catatatcaa aaatagtact
    11461 tttaatggca gcgaaactac aagagagatt aaaagaaatg gatataaaat tagaaatgag
    11521 tgatgaggct gttaaattaa tttctaaatc tggatttgac ttagaatatg gagcaagacc
    11581 tcttaaaaga gctttacaaa aagagttgga agatgagcta tctgaagcaa tcttaaaagg
    11641 taatgtgaaa aagggaagta atgtagtagc aaaagtcaaa gatgaaaaaa tagtttttga
    11701 aactaaataa tttaatagag ttgcatttta aaatattgca aatataagtt ttataatcta
    11761 aacaaaattt taagggcaat gaatctataa taattcattg tccttaatta ttttatttaa
    11821 tctttacata ttgtctttca gatatgctaa aattatagta tagaataaaa aattaaggat
    11881 gataaaagta tggcaaaaat aaaaacaaaa tatgtatgtc aatcttgcgg atatgaaaca
    11941 gctaaatggc ttggtaaatg tccagaatgt acaaaatgga atacatttgt agaagaaata
    12001 gaccaaaaaa gcaccaaaaa agaggtcttt ataatagata aatcttcatc taaaccagtc
    12061 agtataaact caatagagag taaagaagaa gagcggttta caacagatat aaatgaatta
    12121 gatagagtgc ttggaggagg tatagttaaa ggctctttag tgcttgttgg tggtgaccct
    12181 ggtataggta aatcaacact tttaatccaa gtatctagta atgttgccaa tttaggaaaa
    12241 actgttttat atataactgg agaagaatca gaatctcaaa taaaaatgag agctaaaaga
    12301 ttaggtatta actcagaaaa tttatatata tttgctgaaa ataatttaag tataattgaa
    12361 tcttatttag aaagtgtaaa tccagaatta ataattctag actcaattca aactgtcttc
    12421 agtccagaaa tatcctcggc accaggaact gtcagccaaa ttaaagaagg tacttcaaaa
    12481 ttcatgaaaa tttctaaaaa aatgggaatt tcaacgttca tagttgggca tgttacaaaa
    12541 gaaggttcac tagcaggacc aaaattatta gagcatatgg ttgatactgt tctatatttt
    12601 gaaggagaaa gatataatac ttatagatta gtaagagctg ttaaaaatcg atttggttca
    12661 actaatgaac ttggagtgtt tgaaatgagg gatttaggat tagtagaact tgataatcca
    12721 tctaaaattt taatctctga aaaaccaaaa gatgtagcag gttcagtaat aatatctact
    12781 gtagaaggta cgagaccaat gttacttgaa ttgcaagcat tagtttctcc tacaagtttt
    12841 ggtataccaa aaagaacatc tacaggagtc gattacaata gagtaggtat gttattagca
    12901 gtattagaaa aacgtgttgg tttgcaaata caaaatcaag atgtctatat aaacatagtt
    12961 ggaggaataa agataaatga accctcaatt gatttaggga tagccatttc tgtagcatct
    13021 agttttagaa atataccgat agatgaagat atagctgtta caggagaagt gggacttaca
    13081 ggagaagtta gagcagtaag ctttatagaa aaaagaatag ctgagtgtaa aaaacttgga
    13141 tttaaaaaga tagtagttcc tagaagtaat tatgatgttg taaaagagac caaaggcata
    13201 gaaatatggc cagtagataa cttaagacaa gctataaata tagtacttgg gaggaatcaa
    13261 taaatatgga gaattttcta gataataaaa atatgctata tgcattaaaa atgatatctc
    13321 ctggaactcc acttagatta ggtctaaaca atgtactaag agctaagact ggtggattaa
    13381 ttgtaattgc aacaaacgaa gatgtaatga aaatagtaga tggaggattt gctataaatg
    13441 cagaatattc accatcatat ctatatgaat tagctaaaat ggatggagct atagttttaa
    13501 gtggtgatgt aaagaaaata ttatttgcta atgcacaact tatacctgac tattttatag
    13561 aaacatcaga gacaggaaca agacatagaa cagcagaaag agtagcaaaa caaactggtg
    13621 ctatagtcat aggaatttca caaagaagaa atgttataac agtttataga ggaaatgaga
    13681 agtatgtagt cgaagatata tctaagatat ttactaaggc aaatcaggct atacaaactc
    13741 tggaaaaata taagacagta ttggaccaag ctgtaacaaa tttaaatgcc ttagagttta
    13801 atgatttggt aactatttat gatgttgcat tagtcatgca aaagatggaa atggtaatga
    13861 gagttacaag tataattgaa aaatatgtga tagaattggg tgatgaagga actttagtaa
    13921 gtatgcaatt agaagaatta atgggtacaa ccagaataga ccagaaatta atattcaaag
    13981 attataataa agaaaacaca gaaataaaag aacttatgaa aaaggtcaaa aatttaaatt
    14041 cagaagaact aatagaattg gttaatatgg caaaactatt agggtatagt ggtttttcag
    14101 aaagtatgga tatgcctata aaaacaagag gttataggat tcttagcaaa atacatagac
    14161 taccaacagc aataatagaa aacttagtaa attattttga aaactttcaa caaattttag
    14221 atgcatctat tgaagaatta gatgaggttg aaggaatagg tgaaataaga gcaacatata
    14281 taaaaaatgg actcataaaa atgaaacaat tagtcttatt agatagacac atatgatgaa
    14341 ttttaaatga attttactac ttttttcttg aatataatat acactttggt ctataataga
    14401 taagtataac tagggggtga agcattgata aggaaagtaa caagaatatt gtttatttta
    14461 cttggattta caataggtat aacaacctat ttaacattaa tgaaagattt tgaaatatta
    14521 acatttggaa aagaaacata tggttatatc gcagctgtag tagcaggtat aataatagcc
    14581 attttaggat atcttataga accatgggtt gtaaataaag taaaagaaat tgctaaaata
    14641 gtggataaag aattgtcaaa atatcctcag actgatatat tgttagggtc tatgggtctt
    14701 atagtaggat ttgttattgc gtatttatta agtggattag taaatagaat accaatagta
    14761 ggtggaatac tatccttact attatattta ttcctaggat atcttggaat gaaagttgcg
    14821 cttaaaagta aaaatgattt atttaatatt ggaaaactag gaagattagc aaatcctatt
    14881 aaggataaag ataaggataa agaaaataaa aaagaagtaa aagccattcc tccaaaagta
    14941 ttagatacaa gtgtaataat tgatggaaga atagctgata tttgtaagac tgggtttata
    15001 gaaggaaaac ttattattcc tgcttttgtt ttggaggaac taagacatat tgcagattct
    15061 tcagatgatt taaaaagagt aagaggtaga agaggtctgg atatattaaa tataattcag
    15121 aaagaattaa atatagaagt tgaaataagt gagagagact ttgatgatat agcagaagtt
    15181 gatagtaaat tattaaagct tgctcaggtt ttaaatggaa aagttgttac aaatgattat
    15241 aacttaaata aagttgctca attccaaggt gttgaagtat taaatataaa tgagttagct
    15301 aatgctataa aaccagttgc tataccaggt gaagatatgg ttgttcaagt ggttaaagaa
    15361 ggaaaagagg ctcaacaagg tgttgcatat ctagatgatg gaactatgat agttgttgat
    15421 ggaggaagaa aatatatgaa tgagactata aaagtactcg ttacttctgt tcttcaaact
    15481 cctgcaggta gaatgatatt tgcaaaacct aaaaattaat atgttcaaag aaacctataa
    15541 tttgggctgt ttattttaaa tagatggtcc aaatttattt atttataacg ataatgttta
    15601 atgtattctt aaagatgggg atttaaatca gaaagcaggg agaatttatg agaaaaaaga
    15661 ttatatcttt cttagcaaca tttatcataa tattaacatc agcaagtcag tatagttttg
    15721 cagatgatat atcaacacgt ggaaaagtaa tatttataga tatgaataga actagtatga
    15781 gtaatatgtt aagaataaaa tcattaagag aagaactaga caatagaggg tatattggct
    15841 tgatgaatat aagaggcgat aaaggaagtg atgatagaag gtcatatgct agtatgggtg
    15901 ctggtggtag agctaatgtg gctaatgaag aagatattaa ttttgaaagc tcgagtaagg
    15961 atagaaatat agtttttgaa tcagctacag gaaaaagtgc aaaaggtata aataatttaa
    16021 ctataaataa gtctataaat gaaaatttaa actttggaga gtatggttct gtattagggt
    16081 cattaggaca aagtttatct gataatggat taaaagcatc tgtattgggt aattcagata
    16141 taattgaaaa tggtcaactt ataaaaaata gaaacttatg tctaactgca atggatgaat
    16201 atggaagaat acctaatgga aatgtagata ctataaataa aaaagactta tctatgcctt
    16261 atggaatatc aactgattat gataaattga ttgtagagac taaagaagct tacaaaaata
    16321 atgatgtgat ttttgtggag cttggagaca catatagatt agatttatat aaaccaaatt
    16381 taaatgaaaa aacatatgag agtatgaaag atagcataga aaaaaatata gatatatatt
    16441 taaaaaatat attttctatg gttgaagaaa atgacactgt ctacatagca agtgcatttc
    16501 caagtgattt agattataaa aataaaagaa gactttctcc aataataaaa ttaaatggag
    16561 aagggaaagg tatattatca tcttcaacta ctagaagaga aggtattgta acaaatttag
    16621 atgttggggt tgaaatatta gacaacttta atataaaaaa tcaaaatatg gttggaagaa
    16681 aatatgaact tattaacaga gatgataata aagaattttt gatggatgaa tatcaaaaga
    16741 tagtatctat ttcaagcata aggtctacaa tactgaatgg ttttgtaagt atagttttct
    16801 tgtcatggat agttgctatg attgcaattt tatttagaaa gcatatatca aaaaattata
    16861 aagaaacaac attttttata ttaaaggaac ttttaaaaat aggaatagta atgccactgt
    16921 catttatgat aacaccaata atgaatttca aaacaccatt agcaatatct ttaggaataa
    16981 ttataataac acttactata tatcttataa gtaaagtttt aataaagaac gatttaaaaa
    17041 acatgctgtt ttttactgga ttaaccatag taattatggt tattgatgct gggtttggct
    17101 catatttaat gaaaagcaat gttatgagtt atgattgtat aattggagct agatattatg
    17161 gagtaggaaa tgagtatcaa ggtgtggcaa tcggtagtgc aatatttaca tttgcaattt
    17221 tacttacata taaaaatata ccaaagtggt ctataatagt tttttctttg gttatcttaa
    17281 ttacaagtgc atctccaatt atgggagcaa atgttggaag tgctatttct gaatgtgttg
    17341 catttttact atttatacta ttgatatata atgtaaagat agatttcaaa aaaatcgtat
    17401 tattaggtat agcagtagta tttgtattag gagtttttgt agcaatagat atgattttgg
    17461 gaagtaattc acatttaggc atgtttgtaa aagaaattta ctttaatgga ccaggagaaa
    17521 taatacaaac atttagtaga aagatagaga tgaatttaaa gctagctcaa accagtgcat
    17581 gggtaaatat actgttaact ggaataggtg taattttagt tcttatgatt aatcagatta
    17641 gatactttaa gcaactgatg gatgagtatc caattgtatt taaaggattt atagcatcaa
    17701 tagcaggatg tttagtaact cttttagtga atgactctgg gattgtgtca tcagctacag
    17761 cctttattta tgtaattgta cctatgataa cattaagtat aaatctcaca gcattaaaag
    17821 aataagtact gtatgaataa tatgtaaata ttaatacatt aatttaactt ctttatttac
    17881 aaaataaaga agaaagttta taaaaaataa attataataa aaaagagttt atctcaaaat
    17941 tgattattct aatcaaattt taagataaac tttttttatt ataaagataa ataacatata
    18001 acctcatatt ttaggtagat ttttttctta tatgtttagg agtgcattta aatttttcct
    18061 taaaaattct gtaaaataat tttgtattag taaatccatg agcttctaat atactgcata
    18121 aggtatcatt tgtgttaatt aaatctttat aaatatgaga aatacgcaca tcatttaaat
    18181 actctaagta agtttgtccc atatactgtt taaattttct acaaaaatat tctggttgta
    18241 agtgtgctac cttagcgatt ttgttaatag aaataggcat tttgtaatta atctttgtat
    18301 attctaaaac tggttcaaat tttgacaaat caagtactgt ttttttcata tttttattac
    18361 ctacagatat tttaaagttt gtatataatt caaataaaag ttcaaataat aagcttgtaa
    18421 agagcagatt agctgcagga ggagaactaa gtttaatttc tctcatgttt tctaatataa
    18481 gtttaacttt acttgaattt tttttatagt tattgtcatt ggcattaacc ttaaagtcaa
    18541 aatagcaatt tttaaaatca gggatatatt tgtttagaaa agacaatggt acttgcaata
    18601 atatagaggt attttgataa atacatctag ttgagtgaat aattccagaa ttaatgaaca
    18661 tacactcatt agcttttaaa ttataagtgt aaccctccat gttaatttgt aaatcaccag
    18721 aagttatata aattatttct aatgagttat gccaatgatt tgctacataa ctaccactat
    18781 catcaaatag tttgaaatta acatctaaat tatcaatagg ttcaattatt tcatgtgaaa
    18841 aattatttct gttcataata atatcctcct tataatttta aattataaat agtaaattta
    18901 taatgttcaa tattaaaaag ataatataga ctaataaatt agataataaa catacttaag
    18961 aaaacagata aaatcaaata taataactgt atattaaaat aacttaaaaa aattatttta
    19021 ttataagtgc aaaatatacc tatttaaata agataagagg ggaggatttt atgtgtatat
    19081 aaaaggtgta aatttaggag gttggttagt tttagaaaaa tggatgacct caagtttatt
    19141 tgaaggcaca gaagctgaag atgaatacta tttaccaaga cagctttcaa gagaggcata
    19201 tgaatctaga attaagactc atcgtagtga atacatcaca gagagagatt ttgcaacgat
    19261 taaatctatg ggatttaatt cagttcgtat accagtacca tactttattt ttggagattg
    19321 tgaaccattc ataggatgtg ttaaagaact tgataaagca tttgcttggg cagataagta
    19381 tgggcttagt atattgatag acttacatac agttccagga agccaaaatg gatttgataa
    19441 tggaggaata tcaggtatat gtagctggtc acaaaatcca gaatatgtag cgtttacatt
    19501 aaatgtatta gaaagacttg caaaacgtta tgggatgagg catgaattgt atggaatcca
    19561 aattttaaat gagcctataa cagaaagaat gtggaacata atgaatgttc caaatcggtt
    19621 taaggctgta gataaggaga tggcaagggg gagtaaacct aattcattag agtttttacg
    19681 tgatttttat ataaaagcat ataaggtaat gcgtccttat atgagagagg aaaacgttat
    19741 agtatttcat gatgcatttg aattaaaagc atggaaagac tttatgagag aggaagaatt
    19801 taaaaatgtt gttctagata cacaccaata tttgatgtta gctgaagcag atggatgtga
    19861 acaaagtatt gatagctatc tcaaatatat tagagaaaat tatgctaaag atattttaca
    19921 aatgcaaaaa tattttcctg ttatatgtgg ggagtggagc ttatttaatt catatgcttg
    19981 tggtatagat acaaatggag gtcaatcacc tctaaatggg attgaatcaa atatagataa
    20041 attatcaaaa gacgataaaa gagaattata taggaagata gcaaaagcac agcttgatgc
    20101 atggagaaat ggaagtggtc actactattg gaattataag ttattactag atacagttaa
    20161 tgaagaaggt tggataggat gggatagttg ggacctaggc aaatgtgtag ctcaagaatg
    20221 gtatcctata gaatattaaa ctaaaattgg aggaaaagat atgattaaaa accctatact
    20281 tccaggtttt aatccagatc cttgtatttg tagaaaggga gatgattatt atcttgtagt
    20341 ttcatctttt gaatggtttc caggtatacc tgtttatcac tctaaagact taaaaaattg
    20401 ggagttatat actcatattt tgacagatga aacaaaaata gatttgaaga agcttccttc
    20461 atctaaagga atttgggcac cttgtttgac ttattgtgaa gaagaagatt tattttacat
    20521 agtttatgga attatgaact ctatgaatgc aagatatttt gatgttgata attatttaat
    20581 tacatcaaaa gatataaagg gagagtggag tgaaccagta tatctacact catcaggttt
    20641 tgatgcgtct atttttcatg atgatgatgg taaaaagtgg attgcatcat tagactggga
    20701 gactagagaa ggatatgaaa aacctggtgt tatatgttta gtggaatact gtacaaaaaa
    20761 gaaagaaata gtaggatatc caaagagaat atggtcaggg ggaactgata gaggttgtat
    20821 tgaagcacca catataacaa agcgtggaga ttactattat ataatgtgtg cagaaggtgg
    20881 aactggatat ggtcatagtg taactatggg aagggctaaa aatatatggg gaccttatga
    20941 aaaagattca atgaatccaa ttgtaacttc aattccaggc gatttttatg aaagacatga
    21001 ccctgaccat ctaaagccta aatactataa tccagaatca aaacttcaaa aatctggtca
    21061 tggaagctat atagaaacaa catcaggtga agtatattta gttcatctaa catctagacc
    21121 atttgtacca gagttaaggt gtactttagg tagagaaaca gctattcaaa aaatgaaatg
    21181 gacaaaggat aattggcttc gaatggaaga tgaaagtaat ttggcaaaag aatacgtatc
    21241 agaaagtaaa ctagaagaac atttagtttc atctatacca agttttgatg actttgattc
    21301 aaatgaatta ggtttacagt actatgctcc taggatttca ccattatcat ttgcagatgt
    21361 taagtctaga ccaggatatg taaggattag aggacaagaa tcaagaacat cattaaataa
    21421 agtgagcata ttggcaagaa aattaacttc agtatatgca agaataacca ctaagatgga
    21481 gttttaccca gaggtacatc agcatagtgc gggtttgata atgtattatg ataatatgaa
    21541 ttatataaat ttaagaaagt attatagtga aacattgggt caaagtgcac tatcaataat
    21601 acatcttgaa aatggagaga aaacggaatt tttaaatact aggataccaa taaaagatat
    21661 accaatatat cttcgtctgt atatacaagg tcgtaagtca tattttgaat ggagttatga
    21721 tgaaaaaaac taccagagaa taggaaaggt atttgataca actaagtttt cagatgagta
    21781 ctgtaaatat ggagaattta caggtacgtt tataggatta acttgtgcag atcgtgtaaa
    21841 gcataaacac tatgctgatt ttgatttttt tgaatatata gtagatgaat caaaagatgt
    21901 cgattaataa aatcattact aaaattttca gagcaaaggt aaatcttatg ctctgaaata
    21961 agaaacatta gaaggcaaat cttacaaaaa ggagtatggt attatgaata cagaagttaa
    22021 tgttaaagca actacgatta aatatgaaaa gatttcattt tttgaaagaa tttcttatgg
    22081 ttgtggggat ttggggtgca atatcatata ctcagctatg tcagcatttt tgctgtttta
    22141 ttatactaat tatgcagatg ttagtgcagc agctgtaggt agtattatgc ttgtatctag
    22201 gatactggat gggtttagtg acttaacaat gggaattata gtggatagaa ctaaatccaa
    22261 atacggtaag gctaggcctt ggattttgag aatggcgatt ccttttgcta tagcagcagt
    22321 attattattt tctgttccat caaatcttgg tatcacttct aaattaattt atatttttat
    22381 tacatataac ttagtttcta ctgttattta tacagcaatc aatgtaccct atgcaactct
    22441 aaattcactt ataactcaaa accagtatga aagaggagta ttaagtatat ttagaatgat
    22501 attagccact tgtggtacct tactaataaa tggacttaca ctacctctag ttgaatactt
    22561 tggaaataat ttatcagcat ggactaaaac attctttgta tttggaatag catctataat
    22621 ggtatttttt ataacattta ctggaaccaa ggaaagagta aaagcagtta agcaagatag
    22681 gaatgaagta ataccattta aaataggtat taaatcttta tttagaaata agtactggat
    22741 acaaataaca ttatgtttag tttgtatatt tattgtattt gcgataaatg gtggttcatc
    22801 cgtatattat gcaaagttta ttttgggaga tgaaaaatta tttggtccta taaatatggt
    22861 ttcaaatata tcccaaatta tagctatgtt tatggttgct ccttttataa aaaaatttgg
    22921 aaaaagaaat gtattaatag taggttcaat aatattaata tcgtcaaatg ttatgtttat
    22981 aatagttgga caaaatcata taggagtaat atttgcaagt gctataaagg gaataggaag
    23041 tgctggaata gcatcaacaa tgtttgctat tgtttcagat actattgaat atggagaatg
    23101 gaagactgga tatagaactg aggggcttat aaatagtgca tctagttttg ggtttaaagt
    23161 gggaaatgga ttaggttcag caatattagg tgcagtatta tcaattggtg gttatgtagg
    23221 aacatcagct actcaaagtg atttagctat agtatcaata aaagtatgtt ttatatacct
    23281 acctatattt ataacaatat tacaaattat cataatgtct ttttataagc ttgataaaga
    23341 atatagtact atacttaatg aattaaatac taaataatga aaaagtttct tataaagtct
    23401 taagtttaat taaggtctat tgtaaatgtt ccctataaaa actattatat atttatgatt
    23461 attgaagaat ttttagagaa gcgaataata aaaatataag aagactttaa atgatgaaat
    23521 ggttggtttt ttagaaatga tttaataagt tattgttatt taaagcatta taagtggaca
    23581 attaaatttt aatttatgga gatgatattt aacaattaga agtaatatat aatatgcact
    23641 aagaagcaat ttttcaacta tttatgaagg tggtggtaca agaaaagtat cacctttttt
    23701 ttgttataca aaaaaacagt tgacttttta tatgaacccc ttctataatg ttactgaaat
    23761 taagtaaaat gattgaacaa atgtaaagat aattgaaaag cttgattttg gaggaaagca
    23821 aatgaaaaaa attggagact taagattaat gatgaagtgt tgttcacttt attacgaaga
    23881 taatttgaat caacaggaaa tagcaaatca attgggaata tcaagaccaa caatatcaag
    23941 aatattaaaa gaagcttttg aacaaggaat agtaaaaata cagatagttg atgttttgaa
    24001 aaatgattac caaaaaatag agagaagttt ggagagaaaa tataaattaa aagaagtaat
    24061 agtagtagat gataaacagg atgcactaac acaaaaacaa gaactagcaa gagctgtatc
    24121 tgaatattta actagagtag taaaggaaaa tgatataata ggtgtttcaa taggaacaac
    24181 actaaaagaa ataccaagat atgtggaaaa aagtaactgt aaaaatgtta catttatacc
    24241 tctattagga gggattggag acaatgaaat agatattcat gcaaatcaaa tagcagtaag
    24301 tttagcgagg gcttttggag gtgattttaa attattacat gctccagctg ttatgtctga
    24361 cttgtctaca aaagagaaac tttgcaaaga cgaaaaaatt aaagaagtac tagatttaat
    24421 agataaaaca actatagcaa ttgtgggtat aggaaatcct atgagtttaa actcaacaat
    24481 aatggcttct ggatacatga atgaagcaga tatagaagat ttaaaaaaat acaactcaat
    24541 aggtgcaatt tgtttacaag cctttgataa agaaggaaaa acttctatac ttgaatttaa
    24601 tcaaagagta ttaggagtga aactggaaga tttaaaaaag ataaaaagaa caataggtgt
    24661 cgcttcagga gatgaaaaaa tagaggcaat aaaggcatca ttaaaagcga aatttataaa
    24721 tagtttagca attaatcaca gtttagcact taaattatta gaagattgtg actaatagaa
    24781 agatagataa aagttaacag gagggaatgt atatgtcaat atcaaaagaa acattgcttg
    24841 aaatgtataa gagaatgaat caggctagaa aatttgaaga gaaagttagt tggttttttg
    24901 caagaggtat ggttcatgga acaactcact tatctgtagg tcaagaagcg tcaagtgtgg
    24961 cggctgtaat ggctttagaa aaaggagatt tagtatcatt aactcataga ggacatagtc
    25021 aatttatagg aatgggaata gacctaaata aaatgatggc tgaacttatg ggtaaagaaa
    25081 ctggattttg taagggaaaa ggaggctcaa tgcatatagc agatattgag tcaggtaact
    25141 taggagcaaa tggagttgta ggtggaggtc ttacaatagc tccaggagct gcacttacac
    25201 aacagtataa aaaaacaggc aaaatagttt tatgtagttt tggtgatgga gcatcaaatg
    25261 aaggtacttt tcatgaagga ataaatttat cttcaatatg gaaattgcca ataatatttt
    25321 actgtgagaa taatttatac ggtatgtcaa caagcataaa acgtcatatg aatatagaaa
    25381 gtattgccac tagagcagct tcttatggaa tagaaggaat atcaatagat ggatataacc
    25441 caatagaagt atacgaaaca gtacaaaaag ctgctgagaa gtgtagaaga ggagaaggac
    25501 ctgtattaat tgaaagtaga acttatagat ggttaggtca ttcaaaatca gatgcaaatg
    25561 tatatagaac aaaagaagaa atagaatcat ggaaagcaaa agaccctatt gaatttttaa
    25621 aaaattatct tatagaaaat aatctatcta atgaagatga attagataag attcaagaat
    25681 ttgcaaagca atcaatagaa gatgcagtag agtttgctca aaatagccca aatccaaaaa
    25741 tagagtcttt attagaggat gtatatgcag actaagaatg gtaaaggagg aaaataaaaa
    25801 tgagtacaag ggaattaaca tatgcacaag caataaagga agcaatgtca gaagaaatga
    25861 gaagagatga aaatgtaata tttatgggag aagatatagg aatatatgga ggagcatttg
    25921 gtgtatcagt tggtatgata gatgaatttg gaccagaaag agtaagggat actcctatat
    25981 cagaagcagc tatagcagga gcggcagcag gtgcagcagc aactggctta agacctatta
    26041 tggaagtaat gtttatggac tttgtaacaa tttctatgga tgcaattgta aatcaagcgg
    26101 caaaaatgag atatatgttt ggaggaaaag cacaagttcc aatggttgtt agatgtccag
    26161 gaggttctgg aactggttca gcagagcaac attcacaaag tttagaagct tggttttgtc
    26221 atgtaccagg agtaaaagta gttgcacctt ctacaccagc agatgctaag ggattgttga
    26281 aagcagctat aagagataat aatccagtca tatttgttga gaataaattg ttatacagaa
    26341 aaaaaggttt tgtgccagaa gatgattatg taattgaaat tggaaaggca gatattaaaa
    26401 gagagggtac tgatgttaca gtaattactt atggaagaat gttacaaagt gttgaagaag
    26461 cagcagagac tctatcaaaa gaaaatataa atgttgaaat tatagattta agaactttat
    26521 atccattaga taaagagact atagtaaaga gtgtatgtaa aactgggaga gttttaattt
    26581 gccatgaagc agctaagact ggtgggctag gtggagaaat atcagccctg ataacagaaa
    26641 gtgaatcatt tgattattta gatgctccag taaaaagaat ttgtggaaaa gatgtgccaa
    26701 taccatataa tccagagtta gaaaaagcag ttgtcccaag agttgatgaa atagaagaag
    26761 caataaaatc tttaattgtt agataaggag ggatagatat ggtggcaaat aaaataaaag
    26821 ccacaccagc agctagaagt caggcaagaa aagacaatat aaaattagat aggctgattg
    26881 gaagtggaga aaatggaagg attcatttag tagatgtgtt aaattattta aaagataata
    26941 aagcaaatac tactccttta gctagaagaa tagcagaaga tttaaatata gatttagaaa
    27001 ctattgtagg tactggatat aatggaaaga taagaaagtg tgatgtggaa aaattaactg
    27061 caaaagaaac tattgtatca actaatacat ctaaatctag tgagaaaaaa gagctgaaaa
    27121 ttgaaaatga aaattcaagc atgtttaaca cagtagaagg aatatttgaa aagccaaatc
    27181 caatgagagc cactgtagca aaacgaatgt cagaaagtta ttttagtgct cctgtattta
    27241 cattcaatat agaagtagat gctacagaat tgaaggtact tagagcaaag ttaatcgaca
    27301 cagtaaaaga atcaactgga gtaaaattaa ctatgacaga tttaatagta atggcagtat
    27361 ctaaaatact tccaaatcat caagccttaa attcagcatg gacagatgag ggtatattta
    27421 gatataaaga tgtaaatata gctattgcag ttggtttaga tgaaggctta tatgttccgg
    27481 tagtaaaaaa tgcaaacaaa aagtctttaa aagaaatagc aaaagaaagc aaagaacttg
    27541 cggaaaaagt aaagacaggg aaacttatgc cagcagacca agaaggtaat acatttacaa
    27601 taagtaatgt tggaatgtat ggtataacta catttacacc tataataaat atgccttcaa
    27661 gtgcaatctt aggggtaggt gctacacaag ataagtttgt tccagtaaat ggagaggcta
    27721 aaataaaacc tataatgaac ttatcattaa catcagacca tagagtaata gatggaacag
    27781 tagctgcaaa attcttaaaa gatttaaaag aattgttaga aaatccttta tcaatgcttg
    27841 tataggtttt gatattttgt tactgatata aatagtttga atagattata ttattataat
    27901 tgtttcagta aatttaaagc ttttatatat taagaattat ttttaacagt aactatatgt
    27961 gaatatgatt tgcttaatta aaatataaga aaataaatag gaggagagcc tatgtcagta
    28021 gaagtaataa tgccaaaagc aggagtagcc atggaagaag gtactatagt atcttggcta
    28081 aagcaagaag gcgaagaagt aaagattgga gaacctatac tagaaataac aacagataaa
    28141 gttaatatgg agatagagtc agaaggagaa ggtacacttg ctgtaatcat acataaagag
    28201 gaaggtgaag tattaccagt atttacagta ataggtgtaa tagcagaaaa aggggaaaat
    28261 caagaggaag taaaagctaa atatttatct ggaaatgttt caaaagaaga tacagttaaa
    28321 gagaatcaaa atatagaagt taaagaagaa aaaataaaca aaaaagagtg taatcatgat
    28381 tatgatgtag tggtaatagg aggaggacct gggggatatt tatcagcatt aaaagcagct
    28441 cttttaggtg gaagagttgc tttagttgaa gaaaatatac taggaggtac ttgcttaaat
    28501 agaggttgta tccctacaaa gacttatata aaaacagcag aaatattaga agaaattgac
    28561 caattatcaa agagaggtgt aaaagtaact gtagataaag aacaggacat taagaaggcc
    28621 ataaaatata aaaatagagt agttaaaaaa cttacagctg gtgttggcgg attattaaaa
    28681 agtagagatg tagatgtgtt taatcttaaa gctagtgtta aagaagagca taaagtaatt
    28741 ttatcagatg gaaaagtttt agatacagaa aatataataa ttgctacagg ttccaaagta
    28801 aggatattac ctataaaagg aatagaatca aatttaataa taactagtac agaagcatta
    28861 gatttggaaa cagtgccaga agaattagta ataattggtg gtggagtgat tggttgtgaa
    28921 tttgcagaga tattcaattc aagaggttct aaagtaacta tagtagaaat ggaagataga
    28981 gtaatcccaa gaatggataa agagttaagc gaatctttaa aatattctct tagtaaaaaa
    29041 ggtataaatg tattaactaa aaagaaagtt tctgaattta aagaagaagg aaataacata
    29101 ttagtgtgca ttgaaggtga agagccaata aaagcagatt tatgtctata tgctatagga
    29161 agagaagcta atctttctgg catagaggac ttagatatta aaatagataa aggttcaata
    29221 gttgtaaata gtaaaatgga aacaagtatg ccaagcatat atgcagtagg tgatgttact
    29281 ggaggtgtaa tgttagctca tgctgctttt aaaatgggag aggtagcagc ttcaaatgct
    29341 ctaggtgtga ataaagaagt tgacttaggt gcattaccta gttgtgttta tacaattcca
    29401 gaagtagcag cagttggtat tacagaagag gatgctagaa aaaaatataa tgtaaaagtt
    29461 ggtaagttta attttgctgg aaatggaaga gctttagctt ctggtcaaga acaaggatat
    29521 gtgaaggtag tagcagatgc taaatatgga gaaattttag gtatacatat gtttggatgt
    29581 ggtgtagcag aacttataaa tcatgcagca tcatttaaag cgttagagat acctactgat
    29641 gaagcaagtg agttgatatt tggtcatcca tgtacttcag aagctttaat ggaagctttg
    29701 gcagatgtaa atggagaatg tttacactta cctaaaaaat aaatctaaac ataaattatt
    29761 aatttaagtc tattattaat attaatttat atcataaaat tttaaaacac ttacttagcc
    29821 atacccctta ttatgcaaat aatttatttg cataataagg ggtatttttt atggaaaaat
    29881 atagaatata gaatccttgt agtttttgtt gtacaaaaaa gatagtaata taaaataaca
    29941 ttatcatttt agaatgattt caattgtaat taggaaaagt gatttttttg tttttaaaaa
    30001 aagtattatt tgtaaaatat aaaaaaaaat ttcttaatat ttgaataaaa gaatagctta
    30061 atttaaatgt tattacttta taaaaaaata ataagtacaa aaaaaacaat atatggcata
    30121 taagttgcta tataaataat aaggagggaa caattataaa tgaggcaaag aataaaaaac
    30181 ttaattgaaa atgaagataa gaaaaatcct ttaacagatg aagtgatttc aagtaggtta
    30241 aatattcgaa gagaagatgt tacctttttg agaaatgagt taaaaataga agattcaaga
    30301 caaagaagaa aagttgtttt aattaaagct ataaaagata ttttaaaaga agaaaaaact
    30361 ataaataaaa atgatataac aagaagatta aacagcattg ggtttacagt atcaagattt
    30421 acagtcatac aatacttaaa agagattcaa gattctggtg acctaaaact tgataaagag
    30481 agtgatgata aaaaagaaga aataatcaat aatagagaaa ataaagatat agcatttgat
    30541 aaactcatag gtagtagtgg tagcttaagt acaattataa aactagccaa agcagcaatt
    30601 ctttatccac ctcatggtct tcatactatt attatggggc caactggtgt tggaaagagt
    30661 gagttagcag agtgtatgta taaatttgca gtagaatcat ccagattccc taagaatgca
    30721 ccatttatag tttttaatgc agcagattat tcagagaatc cacagctttt gctatcacag
    30781 ttatttggac acgttaaagg tgcttttaca ggagctgatg agaacaaaga aggattagta
    30841 tcaaaggcag atggaggaat attgtttata gatgaaatac atagattacc tcatgaaggt
    30901 caagaaatac tatttcaact gattgataaa ggtatggtca gaaagttagg agaaacaaga
    30961 ctaacacata aaatagatgt aatgattatt tcagcaactt ctgagccaat agattctcat
    31021 ttacttaata catttaaaag acgtatacct gtaacaattg aaataccaga attagttgca
    31081 agaccattaa atgagaggtt tgacattata aataatttct tcttaataga agctcaaaga
    31141 atgggagcaa acatacatat aaagtcagat gtattaaaag ctcttatgct ttacgattgt
    31201 ataggtaatg taggtcaact tagaagtgat attcaagtag cttgtgctag gggactatta
    31261 aaccaactta caaacaaact aaaagaagta aatataagca tatcagatgt acctggatat
    31321 gtaaaacaag gtcttataaa aatcagaaat tgtagaggca aaatagaaaa ttatgtagat
    31381 ggggatttaa ttatagattc aaatcttcaa ggtgagatag aaaagaaaaa tgaagatata
    31441 tatacttttc cagatgaaat atacaaactt actgaaagaa gacatattga tttattggaa
    31501 caaggtttag attttgatgt tataaataga atcattggag gagaattaga aagttgcatt
    31561 caaaaataca taaaacaagt taaaaaagtt tctattagta acaatcttcc agatataagt
    31621 ccaattgttg gaaaatatat cgtagagcta acttatgaag taatcaaaat agcatcacaa
    31681 tacctaagtt cactagaacc aaatctacat ttatgtttag cagttcacct aaaagcagct
    31741 tttgagagat taaaagaagg aaaagtaata tcaaatgctc atttgaaaga aataatagga
    31801 aattatccag tagaatattc tgtagcagaa aaggtagctc aatatttaaa ggaaagatat
    31861 aacataaact taccaaaaga agaaataggt ataatagcaa tatattttaa aatgagtagt
    31921 agaagagata tgtctgaaca aaaaaagata ggcgtacttc ttatggctca cggaagagta
    31981 gcatcttctg tatgtgaggt tacaaataaa ctattaggaa ttaatcacgc aaggtattta
    32041 gatatgccat ttgagaagaa gccggaagaa atgattgaag aagctactac aataataaaa
    32101 gatattgatg aaggtagagg tgtgattata ttagtagata tgggttcact ttcatatttt
    32161 ggagatatta tttctggaaa gactggaatt aatataagga caatatctag atttgataca
    32221 ttgctttcta tagaggttat acgaagagca gttttaccag atttaacaat agacgagata
    32281 gttgatgaag ttaaaggtat agagccacaa gtaaaagaaa aggttagtaa atgtaacaat
    32341 acaaaagtag attttagaaa acctataatt ttaacattat gtattactgg attaggggga
    32401 gcttgtaaga taaaaaaaat cttagaggaa aatattaaaa gtataactga aaaggttgat
    32461 atagaaccta taggtatgat tgaatatcca agtcttgatt tagagataaa acaaataagc
    32521 aatgataaaa atgtaatcgc catagttgga acaattgacc ctcagatagc aaatatccct
    32581 ttttttagtc tagtagacat aaatgataaa gcaaagttaa atcaaataaa taacctaatt
    32641 gatacaaatc aaattggtga aacaaataaa aaatatgaat tatcagattt aatacataaa
    32701 gaagatatat ttgtggattt acaggttaaa tcaaaagaag aagttctaaa atatatgagt
    32761 aaaaaactta gagagaaagg ttatgtgaca aaaaatttct ataaaaaagt agtggaaaga
    32821 gataacttat ttccaactga attatcagat gaaatagcaa tacctcacac agatggaata
    32881 gatgtaatta ggccagctat aagtatagcc atcttaaaga agcctattgt gtggagtaaa
    32941 agtaaagtaa aaataatact tttattagct gtagaccaaa agtgttttaa tccattaagc
    33001 actctactct catttataga aacagatgaa tttaagaaga taaaaaacat taaagataga
    33061 gattttatta gggagatgat attagatgga gtcaatgaaa ataattagta gcaatttaat
    33121 ttttaaaaat attgaagtgt ctaacaatga agatgcatta aagtttttgg gacaacgttt
    33181 gtttgacgaa cagtatgtaa aagaaagcta catacaagct gttgtagcaa gagaaaaaaa
    33241 gtatgctaca gggttaccaa cagaaatata cggagtggca ataccacata cagatatagt
    33301 acatgtaaat gagccaggaa tagcaattgg aatactgaat aaacctgtaa aatttataat
    33361 gatgggtaca gacgatactg aaattgatgt aaaagttgta tttatgttag cagtgaaaga
    33421 gccacaagaa caattgcaac tattagaaaa acttatgact atatttcaag ataagaatat
    33481 actaaataat attgttgact taagtgaaga aagtgtgagt gatttattaa actctaaatt
    33541 gaaaaattaa acatgtaaag tatattaaaa atgtaaaatt aaacatgcaa agtatgttaa
    33601 aaaatataaa aagattatta aagatttcta actatagata tctttaaaat aaaataattt
    33661 caatgggggt gactaatgtg gctaaaaaga tattagttgc atgtggaaca ggagtatgta
    33721 cttcaacaat tgctataaac aaattaaaga aagcacttca agacattggt aaattggata
    33781 tggttaatat tacacaatgt aaagtagtag aagttgcatc aaaagcacct gattatgatt
    33841 taataatttg tactacacaa gtatctagta gtataaaaac accagtgata aatggattac
    33901 cttttttaac tggtgtcgga atggataaat taatcaatga tgtattagaa gagttagaat
    33961 tataattaaa aaaattaagg aacatggtct ctaaaatcat aagttttaga gacttaaaat
    34021 taaataatgg agggtcttta tatggagata ttaaatttca ttgttggtct aggtgcacaa
    34081 gtaatgatgc caataataat atgtattttt ggtttgatat taggtacaaa actaggaaaa
    34141 tctttaagag ctggtttaac tgtgggagtt gggtttatag gtcttaatac tattatagct
    34201 ttgctaacag ataatttagg tccagctaca caacaaatgg ttaagaattt agggctaagt
    34261 ttatcaataa tagatgtagg atggccagca gcatcagcta tagcttttgc atcaacagta
    34321 ggggctttaa ttattccaat aggtcttgtg gtaaatatag ttatgctaat tactaatact
    34381 actcaaactg ttgatgtaga catatgggat tactggcatt ttgcttttac aggagcttta
    34441 gtggcagggg ctactcaaag tgtaatgtgg ggagtatttg cagcagtagc aaatatggta
    34501 attgtattgg taatggcaga tttaactgct ccaggtatag aggaatattt aggtatgcca
    34561 ggtatatctc ttcctcatgg atttacacag gcctttgttc caattgctat agtagtaaat
    34621 aaattattag atctaatacc aggtataaat aagattgaga taaatgctga tacattgcag
    34681 aaaaaatttg gattatttgg agaaccttta ataatggggt ctgtaatagg tgtaataatt
    34741 ggtattgctg cgaaatatga tataaaagga atattacaat taggtgtaac tatgggagct
    34801 gtattaatat taattccaaa gatggcagca ttattaatgg aaggattatt accagtatca
    34861 gaagctgcac aggaatttat tgagaagaga tttaaaaaca gaggcaagat ttatataggc
    34921 ttagattcag cagtaggaat aggtcatcct gttacattat cagttgcact agtgttggtt
    34981 cctttgacta tacttatagc agctatattg ccaggaaata aagttttacc atttgcagat
    35041 ttagcagtaa ttccatttgc acttgtatta atcgtaccaa ttacaaaggg aaatgtgttt
    35101 agaacattaa taataggaat aattataatt acttctggat tattaatcgc aactaactta
    35161 gctccattat ttacccaaat ggctttaaat gctagtttta aaatgcctga aggagcaact
    35221 atgatttcaa gtatttgtga tggtgcgaat ccattaagtt gggtatttgt aaaagttatg
    35281 aactataaag ttattggagg agtagttttt ggagttatag ctttaggaat ggctatttac
    35341 aataggaata gaataataaa tgaaaataaa aagctatcta tagaagaata aggttaaaac
    35401 aattttatta aggagtgtat aggttatgaa aaaatataaa tatgattcta aaagatactt
    35461 actcagtgtt acaataccag gattttttat gattttaata ttgatttatg cactgtttaa
    35521 taactataca aattttaatt tgaatatata tagtttgtta ataattgtct gtacttatag
    35581 tatattcaat acttttatat catcaagtaa tcctaaaaaa attattattg atagcaaatg
    35641 tatacatttt acctcattta tgactacaca taagtatgaa cttaaagata tagaaaagat
    35701 aagaataaga gaattttata ataaaaaaat ttatctaaaa attaatgatg gaaacctttt
    35761 taaaggtagg tattggataa gaacaaatat gtttaataat agtatagcat tatatagcta
    35821 tttcattgag ttggaagagt gcttatatcc agattcacta aaatttagaa ataaaaaatt
    35881 tgagaataag aacatataaa aataaaaaat tgaattgaag cctagcttaa tcttttttga
    35941 taatatataa gtttgaactt tatactaaat ttttagaatt ttcaatcaag ataataacat
    36001 aatagctaag caattagaac acaattaaga tgtataaggc atgtatatga tgaatgggag
    36061 gatttaatta atgttattag agaaattaag aaaagaagta ttacaagcat ctttagacct
    36121 tttaaactat aatttagtga cattaacagg aggaaatgta agtgggagag atgaacaaac
    36181 tggctatata gcaatcaccc caagtggaat ggattataga aatcttacac caagtgatat
    36241 tgttatagta gatgtagatg gaaatattat agatggaaaa tggaaagcat cagtggattt
    36301 atcagaccac ttgtatatat ataagcatag agaagacata aatagtataa ttcatacaca
    36361 ttctacatac tcaagttgtt ttgctatttt aaatgaacct atagaatgcg caagtacaac
    36421 tcttgcaaat gaagtagggg gttcagtacc agttgcaaaa ttttcaccac caacatctaa
    36481 aaagatggga aaatgtgtta ttgaagcaat tggtgacaaa agagcatgtc ttttagcaaa
    36541 tcatggcgta attgcagttg gtccatctgt aggacatgct cttacagccg ctgtaatgtt
    36601 agaagattca gcaaaagtat attacttagc aaaatcaatt ggcacaccag tgctactgcc
    36661 tgatgaagaa attcaaagag cgagagatgt attttttaat gtctatggtc aggataaata
    36721 aattagataa aattttaaaa gtaatatgaa aatatagata ttcaatgata ttaaggagat
    36781 ttataatgat ataataagtt taatagttaa gaagtaaaat atgaaatgta atagtatata
    36841 aattcattta agactaaaaa gaataagata aaataaaaag gagtctgtat ttctatgtat
    36901 tacttattaa acaaaagcac aaatccttac tttaatttgg cattagaaga atatctattt
    36961 ttaaatgata aatataatga tgatattata atcatatgga gaaatgaaga atccattttt
    37021 ataggtaaaa atcaaaatcc atatcaggaa gtatatcatg atgttattga aaagggagaa
    37081 attcctattt taagaagaat atctggagga ggaactgttt atcatgactt aggtaatatc
    37141 aatatgtcat tcatacaaaa agataggcaa cttcatgaga tagatttttt ggagcatact
    37201 aagtttatgc aaaagatgct ttcaacctta ggactagatg tatctataac tgaaagaaaa
    37261 gatttatttt taaatgggaa aaaaatttca ggttctgcac agtctataaa aagaaaaaat
    37321 tctctttatc atggaactct tctttacgat tcagatttaa ataaattaac taaatattta
    37381 aattcaaata aagcaactga atcaaatgca actaaatctg tttcaagtaa agttacaaat
    37441 attaagtttt tactcgaaaa agatataaat tactttttag attattgtgt tgaatattta
    37501 aaatctaata taaagaatat aaaagaactt gaattggatg aagaagatat taaaagtgta
    37561 tacaatatag gagaagataa atataaaaag atagagtgga catatggtaa aacgccgaaa
    37621 tttcaagtag tgctaccatt taataatgaa agtaaaataa atatacatgt taacagatgg
    37681 aaagtatcaa aattttttat ttcttatgat gataatgtaa ttaatatgga caagttgtta
    37741 gatttgaatt tctttaaaga agaaataaaa gagagtattt tagaaaatta tccacattac
    37801 atagatttaa tcaatttaat attttaatat aaaacctcta atttgataaa attgtcttat
    37861 atcaaactag aggtttttaa tattcttgca tattaacaac cattacaaaa ttgtaaaatt
    37921 aaaaacaaca ctattgaaaa atcaatatat atttgtttaa atatatgtgt ttttcattag
    37981 caaatagaca ttaactattt atacaactga aaatttaata tataattaat cagatggtta
    38041 aaataaaaat attaaaagtt ttaataaatg atggttaaaa ataaaatacg aggtgatacc
    38101 atgtatagtg ttataattgt tgcagcggga agcggaagga gaatgaactt agatataaac
    38161 aaacagttca taaaattaag agaaaaagaa attattgctc atacaattca agttttttat
    38221 gaaaatataa atatagatga aatagtggta tgtataaaaa aagaagaaga agattttttt
    38281 aaagaaaata taataaataa gtataacttt aaaaatataa aaatagcata tggcggaaaa
    38341 gaaagacagg actcaatata taatggttta aaaaaattag ataaaaattg tgatatagta
    38401 cttatccatg atggagcaag accatttgtc gaccatagaa taatcaacga atctataaag
    38461 gtagctaaag aaaaaaaggc tgtggttgta ggtgttccag taagtgatac tataaagata
    38521 gtgtcagatg gaacagtaaa agaaactcca gaaagaaatt tgctgtgggc agcacaaacc
    38581 cctcaaacct ttgaatataa tctcataata gatgcttatg aacaagcata taagaacaat
    38641 tattatggta ctgatgattc aatgttggta gaaaatatag gtcaaagtgt cactatggta
    38701 atgggttctt atgaaaacat aaaaataact agtccagaag atttaaatat agcagaacaa
    38761 atattaaata tggaaaaaag agatgaagtt aacagtagaa ggagaattat atgagaatag
    38821 gattaggata tgatgtacac aaattaacag aggatagaaa acttataatc ggtggtgtag
    38881 agataccaca cgataaaggt ttgttgggtc attctgatgc tgatgtattg atacatgcaa
    38941 taatggattc tattttaggt gctctagcat taggagatat agggaagcat ttcccagata
    39001 cagatgaaga atataaggga gcagatagta tgaaattatt agaacatgtg tataatctaa
    39061 taacaagtaa aggatataaa attggtaata tagacagtac tataattgct caaagcccta
    39121 agatggctcc atatattgag agtatgagaa gtaatatatc aaaagtttta aacacagaca
    39181 ttgataatat aaacataaag gcaacaacag aagaaggtct tggttttaca ggagcaaaac
    39241 agggaatagc ttcacaaagt atttgtttat tattgttgac aagtcagaat aattaatata
    39301 ttattagtaa taagtttaat ggatatcaag taataggcct atatagaata tctataatgt
    39361 atgaatttaa gtgaaattaa tataaatata aaattagcta tgataaagaa atagtaaaat
    39421 gatgaaatct tcacagagag aaaacggtgg tgagagtttt caagaggaag tctttgaacc
    39481 tgtcttttag cttaaaggta taaaaatctt tacggtagga accgttatat tcccaaaaga
    39541 gagctattca tatagctaac tagagtggta ccgcggaaat taaacctttc gtctctatac
    39601 ttgtatagag atgagaggtt ttttatattt tcaggaagtt aatattcagg aggaatctat
    39661 taatgaaaat gtcaaaaatg tttatgccga cattaaaaga aatcccagca gatgctgaga
    39721 ttacaagcca tcaattaatg gttaggtcag gcatgataaa aaaaatgact tcaggtgttt
    39781 ataaccaatt accaatgggg ttaagagtgt tcaaaaaaat tgaacaaata attagagaag
    39841 aattaaataa aaaagattgt caagaaatac tttgtgcagc cttacttcct tctgaattat
    39901 ggaaagagtc tggaaggtgg actgctatgg gtgaagaaat gtttagacta aaagacagaa
    39961 cagaaagaga gtactgtctt gggccaacac atgaagaagc attcactgat ataataagac
    40021 aagaaattac ttcatataaa caattgccac tgaacttgta ccaaatacaa gtaaaatata
    40081 gagacgagag aaggccaaga tttggtgtta tgagaactaa gacttttaca atgaaggatg
    40141 cctatagttt tgatgtagat gataaaggtt tagataagtc atatcaagat atgtttgatg
    40201 catatgtaag tatatttaat agatgtggat tggagaatag ccctgtacag gcagattcag
    40261 gagctatagg aggttctaca tcagccgaat ttatggttaa gtcagaagtt ggtgaagatg
    40321 aagttgtatt ttgtagtgga tgtgattatg cagccaatgt agaaagagca gaatcttgta
    40381 atctagcatc acaaaaagaa gaaatgaaag aattagaaga ggttcataca ccaggagcag
    40441 ctacaataaa agagttagaa gaatttttaa aaacttctcc agataagttt gctaaaactt
    40501 tagtatatga ggcagatgga aaaacagttg tagtcgttgt aagaggagat agagaagtta
    40561 atgaaatcaa agtatctaat gccataggtt ctgttataga atttgctctt gctacagatg
    40621 atgtagtgag aaaagtaact aatgcagaag taggatttgc aggtccaata ggtataaatg
    40681 ctgactatgt atttatagat aaagaaattg ttgaacaaag aaacatagtt gttggggcta
    40741 ataaaactga gtaccatata aaaaatgcta actatggaag agattttgaa ggaatagttg
    40801 gagactttag aaatgttcaa gaaggcgaca agtgtatagt atgtggtaag ccacttgaaa
    40861 tagctagagg tgttgaagta ggacatatat ttaaactagg aactaagtac tcagaatcta
    40921 tgaatgcaaa cttcatagac aaagatggaa aatcaaagcc tatagtaatg ggatgttatg
    40981 gaataggtgt tgaaagaaca gcagcagcta taattgaaca acataatgat gagaaaggta
    41041 taatatggcc tttgtcagta gctccatacc atgttgtgat agttccagca aatatgaaaa
    41101 atgaagaaca aatatcaata gctgaaaata tatataatga tttgcaagct atgggtgtag
    41161 aagttttatt agatgacaga gatgaaagaa taggtgtcaa attcaatgat tcagaattaa
    41221 taggaatacc tatgaggatt acagtaggta aaaatattaa tgaaggtaaa gtagaattta
    41281 aacttagaca taaagaagat aaagagataa ttgacataga agaaataaat gaaaaagtaa
    41341 aggcagagtt tatcaggaat aatgttaggt taggataata acttaaataa gatattgtat
    41401 agataatcaa agttgtactt tatagacacc attttatcta tatataagga tataaaatgg
    41461 tgttttttta taaaacaaat aaatgaggag gtaattaaaa tggcaaaaaa tgaaaaacaa
    41521 tttgttgaag aaataacaaa gatggaagat gattttccac aatggtacac agatgtaata
    41581 actaagactg acttagttga ttatgcacca gtaaaaggtt ttatggttgt aaagccatat
    41641 ggatatgcat tatgggaaaa gatgcaagaa tttatggata aaaaatttaa agaaactgga
    41701 cataaaaact gttattttcc attattaata ccagagagtt tattaaacaa agaagcagaa
    41761 cacgttgaag gttttgctcc agaagttgca tgggtaacac atggaggaaa taaaaaatta
    41821 gaagaaagat tatgtgtaag acctacttca gagactataa tatgtacaat gtatgcgaaa
    41881 tggttaaaat catatagaga attaccatat ctttataatc aatggtgctc agttgttaga
    41941 tgggaaaaat caactagacc tttcttaaga acatctgagt ttttatggca agaaggtcat
    42001 acattacatg aaacagcaga agaagcacaa gaagaaacaa tacaacaatt agaagtatat
    42061 aaagcgctat gtgaagaatt acttgcaatg ccagttgtag ctggtcaaaa aagtgaaagt
    42121 gagaaatttg caggtggtga aagaacatat actatagaag ctatgatgca tgatggtaaa
    42181 gcattacaat caggtacaag tcacttccta ggtcaacact ttactaaggc ttttgatatt
    42241 acttttgcag atagggaagg aaatttagct aatccatacc atacatcttg gggagcatct
    42301 acaagactta taggtggact tataatgact catagtgata acagaggttt agtattacca
    42361 ccaagagtag ctccaattca agttgttata gttcctatag ctgctaaaaa aggaaatgta
    42421 atggagactg ttgataaaat atatgctgat ttaaaagcta agggagtagc tgtagaagtt
    42481 gatgatagag ataactatac aactggatgg aaatttaatg agtgggaaat gaaaggtgtt
    42541 ccagtaagag ttgaaattgg accaaaagat atagaaaata atcaagctat ggtatttaga
    42601 agagatactt tagaaaagga ttctatgcct ttagaaggat tagctgatgc tatatgtgat
    42661 ttatttgatg ttatacacaa tgatatgttt gaaaaagcaa gaaaacatag agaagacaat
    42721 acatctatag ttgaaaatat ggatgagttt agaaaagctt tagaagaaaa gccaggattc
    42781 ataaagacta tgtggtgtgg agatgcagaa tgtgaagcta agattaagga agaaacagga
    42841 gcaactataa gatgcttacc ttttgaacaa gaaaacttag gtcacaagtg tgtgtactgt
    42901 ggaaaagaag cagatagtat ggtagtaatg gcaaaagctt actaaaatac atttaaataa
    42961 aaactggggt agaaagtatt taatacttta tcccagtttt tttgtttttt atgagtaata
    43021 aaatgtattt gtaactatgt cataagataa gtcat
//
LOCUS       DAEOSP010000013        42374 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.013, whole genome shotgun sequence.
ACCESSION   DAEOSP010000013 DAEOSP010000000
VERSION     DAEOSP010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.013"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            276..1490
                     /locus_tag="KRH54_000526"
     CDS             276..1490
                     /locus_tag="KRH54_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8578199.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(1910..2431)
                     /locus_tag="KRH54_000527"
     CDS             complement(1910..2431)
                     /locus_tag="KRH54_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8578200.1"
                     /translation="MKFEWLRTSENKLYYKAMELYKISFPFHEQRKSSLQAEILKNKE
                     YQFNLIYDKNQFVGIILCWETDDFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHKKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            2787..3584
                     /locus_tag="KRH54_000528"
     CDS             2787..3584
                     /locus_tag="KRH54_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG8578201.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            3694..4284
                     /locus_tag="KRH54_000529"
     CDS             3694..4284
                     /locus_tag="KRH54_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBG8578202.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            4350..6047
                     /locus_tag="KRH54_000530"
     CDS             4350..6047
                     /locus_tag="KRH54_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG8578203.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            6261..7100
                     /locus_tag="KRH54_000531"
     CDS             6261..7100
                     /locus_tag="KRH54_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBG8578204.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            7617..9473
                     /locus_tag="KRH54_000532"
     CDS             7617..9473
                     /locus_tag="KRH54_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG8578205.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    9556..9796
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    9862..10097
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10182..11399
                     /locus_tag="KRH54_000533"
     CDS             10182..11399
                     /locus_tag="KRH54_000533"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG8578206.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            11516..11998
                     /gene="cdeM"
                     /locus_tag="KRH54_000534"
     CDS             11516..11998
                     /gene="cdeM"
                     /locus_tag="KRH54_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBG8578207.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            12263..13528
                     /gene="hisD"
                     /locus_tag="KRH54_000535"
     CDS             12263..13528
                     /gene="hisD"
                     /locus_tag="KRH54_000535"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBG8578208.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(13731..14483)
                     /locus_tag="KRH54_000536"
     CDS             complement(13731..14483)
                     /locus_tag="KRH54_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8578209.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYAE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIYIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(14601..14870)
                     /locus_tag="KRH54_000537"
     CDS             complement(14601..14870)
                     /locus_tag="KRH54_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578210.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            15345..16439
                     /locus_tag="KRH54_000538"
     CDS             15345..16439
                     /locus_tag="KRH54_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG8578211.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVDS
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            16441..17391
                     /locus_tag="KRH54_000539"
     CDS             16441..17391
                     /locus_tag="KRH54_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBG8578212.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            17384..18568
                     /locus_tag="KRH54_000540"
     CDS             17384..18568
                     /locus_tag="KRH54_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578213.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYKKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISSYFKSKGLKIGIDAFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            18581..20077
                     /locus_tag="KRH54_000541"
     CDS             18581..20077
                     /locus_tag="KRH54_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG8578214.1"
                     /translation="MSKILEIQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            20100..21050
                     /locus_tag="KRH54_000542"
     CDS             20100..21050
                     /locus_tag="KRH54_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8578215.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMFLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            21071..22033
                     /locus_tag="KRH54_000543"
     CDS             21071..22033
                     /locus_tag="KRH54_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG8578216.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            22405..23037
                     /locus_tag="KRH54_000544"
     CDS             22405..23037
                     /locus_tag="KRH54_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG8578217.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDSLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            23448..23540
                     /gene="kdpF"
                     /locus_tag="KRH54_000545"
     CDS             23448..23540
                     /gene="kdpF"
                     /locus_tag="KRH54_000545"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBG8578218.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            23581..25263
                     /gene="kdpA"
                     /locus_tag="KRH54_000546"
     CDS             23581..25263
                     /gene="kdpA"
                     /locus_tag="KRH54_000546"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBG8578219.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            25288..27345
                     /gene="kdpB"
                     /locus_tag="KRH54_000547"
     CDS             25288..27345
                     /gene="kdpB"
                     /locus_tag="KRH54_000547"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBG8578220.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            27364..27990
                     /locus_tag="KRH54_000548"
     CDS             27364..27990
                     /locus_tag="KRH54_000548"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBG8578221.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDVRFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEQFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            28227..29135
                     /gene="cysK"
                     /locus_tag="KRH54_000549"
     CDS             28227..29135
                     /gene="cysK"
                     /locus_tag="KRH54_000549"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBG8578222.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            29162..29752
                     /gene="cysE"
                     /locus_tag="KRH54_000550"
     CDS             29162..29752
                     /gene="cysE"
                     /locus_tag="KRH54_000550"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBG8578223.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            29912..30100
                     /locus_tag="KRH54_000551"
     CDS             29912..30100
                     /locus_tag="KRH54_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG8578224.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(30154..31023)
                     /locus_tag="KRH54_000552"
     CDS             complement(30154..31023)
                     /locus_tag="KRH54_000552"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBG8578225.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTKMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(31220..32464)
                     /locus_tag="KRH54_000553"
     CDS             complement(31220..32464)
                     /locus_tag="KRH54_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578226.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFNEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            32755..33066
                     /locus_tag="KRH54_000554"
     CDS             32755..33066
                     /locus_tag="KRH54_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG8578227.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      33159..33251
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            33351..34145
                     /gene="thiD"
                     /locus_tag="KRH54_000555"
     CDS             33351..34145
                     /gene="thiD"
                     /locus_tag="KRH54_000555"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBG8578228.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            34169..34957
                     /gene="thiM"
                     /locus_tag="KRH54_000556"
     CDS             34169..34957
                     /gene="thiM"
                     /locus_tag="KRH54_000556"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBG8578229.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVKGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            34980..35612
                     /gene="thiE"
                     /locus_tag="KRH54_000557"
     CDS             34980..35612
                     /gene="thiE"
                     /locus_tag="KRH54_000557"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBG8578230.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            35751..36053
                     /locus_tag="KRH54_000558"
     CDS             35751..36053
                     /locus_tag="KRH54_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8578231.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(36119..36874)
                     /locus_tag="KRH54_000559"
     CDS             complement(36119..36874)
                     /locus_tag="KRH54_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8578232.1"
                     /translation="MGMLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMN
                     NININNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQL
                     LEAILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLP
                     NEIVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETI
                     LIGDIMPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(36862..37824)
                     /locus_tag="KRH54_000560"
     CDS             complement(36862..37824)
                     /locus_tag="KRH54_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8578233.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNALSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDAIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(37828..38286)
                     /locus_tag="KRH54_000561"
     CDS             complement(37828..38286)
                     /locus_tag="KRH54_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG8578234.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNFPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            38641..38997
                     /locus_tag="KRH54_000562"
     CDS             38641..38997
                     /locus_tag="KRH54_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8578235.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            39069..39785
                     /locus_tag="KRH54_000563"
     CDS             39069..39785
                     /locus_tag="KRH54_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8578236.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            39778..40602
                     /locus_tag="KRH54_000564"
     CDS             39778..40602
                     /locus_tag="KRH54_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8578237.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIITLFRKYYSLIGFQFNNLVAGLLSFLIIMFILNGISYLLIKKKVSL"
     gene            40614..41813
                     /locus_tag="KRH54_000565"
     CDS             40614..41813
                     /locus_tag="KRH54_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578238.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYANILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIETIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDNKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            41822..>42374
                     /locus_tag="KRH54_000566"
     CDS             41822..>42374
                     /locus_tag="KRH54_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578239.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVKVTGFSSFRNIMGSTESWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEIL"
BASE COUNT        16541 a         4481 c         7304 g        14048 t
ORIGIN      
        1 tatttaaaat tccttgaaag tttaaaagtt taaatatagt aagttgtaat aaagagaaaa
       61 tctaaatatt taaatgctat ttaatgtgaa tctattaatt aggttatact taaacttaat
      121 taatagattt tatatttcaa gccaatgaga tagggtttta gattaaaata taaccaatgg
      181 ttaaattaca gacattttgt taactttccc tcgatttact agtaaatatt ttttttatat
      241 acttaaatta caacagatgt actagggagg tatatatgca aattaaaata ggtaaagtca
      301 tacagtgtct tagaaaagaa cgaaatttaa ctcaagaaca attagcaaag tttataggtg
      361 tgtcaacacc agcagtatct aagtgggaga gtggaaattc ttacccagat atagaattat
      421 tgcctttatt agcagacttt tttaatgtgt ctattgataa gcttttaaat tacaagattg
      481 atttaagtga agaagaagta atgaaaattt ataaagaatt agaatcaggc tttgctagaa
      541 ttgaaattga tttatcaaca gaagaaccaa aagaagaatt tagacaagat ttagaatctg
      601 taaaaaagct ttccaatatg tatatagaga agtatcctaa aagctattta ttgaagctaa
      661 ggatatgttc gctgtatcag atgtattcat ataaatttgg taagagtgaa cttaatgaca
      721 gagtaaaaga aacaacgaat attcttgaag atatagtgag aaatacagat gatatccaaa
      781 taaaagaaac agctcttatt atattatcaa acgcatattg tatgttggag gattatgaaa
      841 aggcagagtt gtatttaaat atgatacata agtcaatagg ggatacaagt gttaatttag
      901 ctatgatata tttaaagcaa aatagacttg aagaagcaga aatattgctt caaaataaat
      961 tgtttagtaa tgtatttaat ataagcatgg attgtaaggg aataattaat gtatacaaaa
     1021 atcaatacaa ggaattaaag aaaaaattgg agaatagaaa ttttaataaa aatgcaattg
     1081 agagagaaat ggagtatata aaaaataagt tgctggggta tgcaaatctt tctttagaaa
     1141 taaaaaaaat gctcagtgaa gataaaggtg cattttttag tatgtatatg gattatatgg
     1201 aattatctct aattttttta ttttttaaca tgaaagaaga agcaaaaaaa gctttatata
     1261 gtctaaaaga aatattagaa aaatatccta tacatgaaag tttggatgta agtcaaatga
     1321 gattttttga taaggtagag tctaaaaatt tatatacttt taatatatat acaaacttgt
     1381 taattgtact taatgatgac agttataatg agcttagaga agaaccaatt tttaaggatg
     1441 taattgaaaa gattttagat atggaaaaaa tgttgaaaaa taaagaataa gtaaaaattt
     1501 aatataggac ttaaagatta atcaaattaa aagaaagtgt ttcaaaataa aaatttgaat
     1561 cacctttttt gtcggtctta aacagttatt tagaaaaata gaggtttcaa aatagaaatc
     1621 tgaattatct gtattattac acaaaaatag aaacctaaat ttataaaaat agaataaagt
     1681 ttgttatata aaatgtaatt ttgtctataa aatctcacct gttttcttaa ctttaagcgt
     1741 gaaaaagagg atgtatcatg aattttttag ttcatgatgc atcctgcttt gtatgaaatc
     1801 caatatatct ttattattta aacaatggaa aagagtctta tttatatttg gagacatctc
     1861 ttttatttaa ttatgaagtt ttatacagtt caataaatag gaattatatt tagaaactca
     1921 tgacattatt tcttagatag tgattaaatt tatcatattc cattttagat aacttttctg
     1981 ggtaagacat tacaatcagg ttatgcccat taaatttttt atggtatggt ggatgaatgt
     2041 gttcataatt atttgtctta tattttactc tttcatagaa cgccttgcga taaattgaaa
     2101 tctcatcaat tggaaggtca atttctaaga taatagtttt tcctttctta tttaaaagct
     2161 ctaacgctct ttgcccatat ctattattgc gcatttctgg aaaaatgcag aaatgttcta
     2221 catagataaa gtcatctgtt tcccaacata aaattatgcc gacaaactgg tttttatcat
     2281 aaattaaatt gaattgatat tctttatttt tcaaaatttc tgcttgcaaa gaagattttc
     2341 tttgttcatg aaatgggaaa ctaattttat ataattccat agccttgtaa tataatttat
     2401 tttcactggt tcttaaccat tcaaatttca ctttactcct cctaatcacg aaaattccaa
     2461 tttatttatg agttcatttt accatagtca gtatttgcat acaatgtgtt ttaagttgag
     2521 tgtcatttct ttcttttatt tatgcttata tctcatagtc taagagataa ttgcttaaga
     2581 cataactaaa aactattata ttaagctttg ataatctaaa aacagtatat ttatggactt
     2641 ttctaggtct aaaagtaatc ttaataattt tagaagttac aaaaaagtta cataaatttg
     2701 gaaagctata atatacatat ataatataag gttaaaactg taatgaataa tattaattta
     2761 aaatttgaac atggaaggag attattatgg agaaaagaga aaagaggata ataatatcat
     2821 ctttattaag tgtatcaatt ttaatggggt tggtaagtat atattctata ttaaataaag
     2881 aagacataat tttaactgtt aagggtcaag agcaaaaagt atcttccttt aagaaaacag
     2941 ttgaggaact tttggatgaa cagggtgtaa agtataattc tgaagataag attaatccaa
     3001 gtttagatac agaactaaaa gatgatatga aaataaaagt tgttaaggta actaaaagta
     3061 aaaaagaaga gattgaaaaa attccatttg atacaaagca tgtaaatgat agtaatttgt
     3121 taaaaggaaa atctaaagtt tatcaagaag gtcaagaagg agaaaaaaaa ctagtctata
     3181 atttaactta ccatgatgga aagttagtca aaaaagtctt atcaaaagaa gtaatatcta
     3241 aggagccaac tacaaaaatt ataaaatatg gaactaaaga aaaagtacta atagcatcaa
     3301 gaggagcaaa tataagagga ggcaaacata tgaaagtggt tgccactgca tatgcaggag
     3361 atacaataac atctactggt acaactccaa gatggggtgt tattgcagtt gacccacgtg
     3421 taataccata tggaacaaaa gtatatatac ctaaactggg tatgactttt gtagcagaag
     3481 attgtggagg tgcaattaaa ggtaatagaa tagacatttt tatgaacagt gaaggaaaag
     3541 cttcaaattg gggaaggaaa agtatagata tatatctaca ttaaattagt gaatagaata
     3601 actttacttg aaggacaaaa aagtataaaa tatgaattaa ctaaactgtt agattattct
     3661 taaaacataa aaatatatct aaggggggat aagatgaaaa tagtctgctt aggagacagt
     3721 ttaacgtatg gatttggagt atcacgaagt aattcatgga caaacattgt aaataaagag
     3781 actcgattag aaatagtaaa taagggtata aatggtgata caacaagtgg tatgttagct
     3841 aggtttaatg aagatgttgt aaaaaattct cctgatatag tttttataat gggtggcacc
     3901 aatgatttta ttgctggtgc gggaaatgaa attattaatt ctaatataat ggctatggtg
     3961 catcagtcat attcaaaaaa tattatacct ataataggta taccattaaa accagatatt
     4021 ccaaatgtta gggaagattg gagttgtttt actgatttta atatagtatc tcaaaactta
     4081 gaatcatata gttattggat aaaaaaattt tgtatgacat ttaatactaa tttcgtagat
     4141 ttttattcag aatataataa aaatatggaa ctagaaggat ataaaaaatt atattttgat
     4201 gggttacatc caaccaaaga gggtcataga ataatggcag acatttttat taattcaatt
     4261 aataagtata tagaagagtt atagattaca ttaaaatgct tgtttatgcg tcagttgcaa
     4321 taaatacata cagtagagag gggtttatta tggattatac taaaacttat gaagagtgga
     4381 taaagggttc atattttgat gaagatacaa agctagagtt ggagaatata aaaaataatg
     4441 aaaaagaaat agaggataga ttctataagg atttagaatt tgggactgcg ggtcttagag
     4501 gaataattga agcaggaacg aatagaataa ataagtacac agttagaaga gctacttttg
     4561 gattggcaaa ctatatatta gaaaacacaa caaaagaaga aacaagtaga ggagtagtaa
     4621 tagctcatga taatagacac aagtctagac aattctgtat agaatctgca aatactttgg
     4681 cagcatgtgg tataaaagca tatatatttg atagtttgag aactactcca gaattatctt
     4741 ttgctgttag aagcttaaat gcaatcgctg gtatagtcat aacagcaagc cataatcctc
     4801 cagaatataa tggatataag gtatactggg aagatggagc acaagttatg ccagagattg
     4861 caaatgcaat aacagagaaa gtaaatagta tacatgatta tagtacaata ccaaccttaa
     4921 cggaagctaa taaaaatttg gtagttttgt tggacgaatc acaagatact aaatttatag
     4981 aagcagtaaa aagccaaatt ataagaaaag atttagttaa aaatgtaggt aaaagcttta
     5041 agatagttta tacacctctt tgtggaacag ggaatgttcc aataagaaga gcattgaaag
     5101 aagttggttt tgaaaatata attgtagttc cagaagaaga aaatccagac ccaaattttg
     5161 cagggcttga ttatccaaat ccagaagaga aaaaagcttt aaatagaggt atattactcg
     5221 caaaagaaaa aggagcagac cttgtaatag caactgaccc agattgtgat agagtaggtg
     5281 tagcagtaaa aactactaca ggtgagtatg ctttacttac aggaaatcaa attggtggta
     5341 tgctgacaca ttatataata gaaggattaa aagaaaataa taaattaaaa gaaaatccta
     5401 caataataaa aaccatagtt acatcagaat ttggagcaga tatagctaaa gcaaataatg
     5461 ttgatgtatt aaatgttctt acaggtttta aatttattgg tgaaaaaata aaattatttg
     5521 aacaaaataa aaatagaagt tatgtatttg gatatgaaga aagttatggt tacttagttg
     5581 ggactcatgc tagagataaa gatggtgtag tttcttcatt gttaatatct gaaatggcag
     5641 cattttacta ttcaaaaggt atgagtttat atgatggttt aatagaactt tataaaaagt
     5701 atggattttt caaagaacag actatatctt tgactttaaa aggtatagaa ggtgtagaaa
     5761 agataaaaga aataatatct tattttagag aaaatcaaat tgattatata aatagtatta
     5821 aagtagttga taaaaaagat tataaaaatg gtatagataa tcttcctaaa tctgatgttt
     5881 taaaatactt tttggaagat gaatcttggg tagctataag accatctgga acagaaccaa
     5941 aacttaaatt ttacatagct gtaaaaggag ctagtgatat tgaagcagat aaaaagattc
     6001 aaggtttgaa aaagtatata gatgacatgg tcgaaaaatt aaaataaaat attattattt
     6061 tataagataa ttttgtaata atatttattt agctgaaaat tataatttac tttcattgag
     6121 tagaatatgg aaatgttagt agaattgtta ttttcagctt ttatttgtgt tatttttact
     6181 taaataatta tattatagaa tgaatctgag tataattaat attgtataat gaaattaatt
     6241 tgatagaaaa ggggatattt atgtttggta tagataagaa attgattgaa tatataattg
     6301 caaaatccaa ttacagtgaa ttaagttcaa agtcagcaga agtatctttc ttcttaatgt
     6361 tgtccatatt tcctttttta atatttacaa taagttctat agcctatata ccgattcttc
     6421 atttaaataa gtatatagcc ttgtttagaa atatgatgcc agaaggtgct tttgctgtgt
     6481 tatcttcaat catagtttct gcaatagaca atagaaacct gaaattttta gcagtcagtt
     6541 ttgtacttac tatgtggaca ttttcaagag cagtaaaagc tcttattaaa ggaatgaata
     6601 gagcctataa agttaaagaa actaggtctt tttttaaaat tttatctata tcatttttat
     6661 ttacaataat gcttttggtc cttatttttt tatctatgat atttttagtt tacggggaaa
     6721 aaataggtta ttttattttt aatcttgtag gattggatga gatatttata aaaatatggg
     6781 atattctaag atacactgta ggtataataa caataatagt gatatttaca cttttatata
     6841 aatacactcc aaataaaaag ttaactatta aagaatctgc gccaggtgct atttttgcta
     6901 cttttgcttg gtttttagta tcatttttat attcttatta tacaaattac tatgctaact
     6961 atgaagtaat ctatggaagt atagctgaaa taattgtact tatgacctgg atgtatttta
     7021 gtagttggtc tatcgttatt ggctatgagg taaattcaag attgtatttt agaaagataa
     7081 gacatgaaat gttaaagtaa agtatttaaa ataaaagaga gtatttagta ttataaatgt
     7141 aattgaatta ataaagaaat attgacattt aagttaaaaa gtctagaatt tttctgggct
     7201 ttttttattt ttagtataac ttgacaaatt ttatttaatg ggatagtttt aatttataat
     7261 catctttaaa aagatgacta ttgataagta agagataaaa ttggtaagta tcaaaaaata
     7321 tactaaataa aaattatagt cctaatgagt taatttctgt attttcaaat aatgcagtat
     7381 ttagtcatgt gtttttatat gtgcctgtat ttctaattac caatatatta gaataagcta
     7441 ctgtaattag gtttagaaaa aagcgacata aataattata aacaaataat aaattacata
     7501 aaattaaatt tattcacaag tatatcttat aaaatatgtt ataattaata gaaaattgtc
     7561 atataattaa ttaagtagta ttattataaa taacattgaa aggtagtggt tagaatatgg
     7621 atacccataa taaatatgta aattttataa aaaatatacc tgtacctttt ttatattgta
     7681 gaattgtaaa gagacaagaa gatatagaat atcgagtaga atatataagt aagggtatgg
     7741 gcaaagtatt acaattagaa gaaggtattt gtgataaaaa tatattagat gtattgcctg
     7801 tatttaagtc taaaaaatat ttcaaagagt tgttctcaaa tgaagtggat tgtataaaaa
     7861 ggtacattcc gacacttaaa aactggataa atataaaaaa acagataata ggggattcat
     7921 acataatttt gtattttggt aaaattgtat ttgattatag acagataatc gattcctttg
     7981 ataaaaagga aaaagtagct tatattaaag acgaagaagg catatatata gattgtagtg
     8041 aaaatttaat accaatatta aataacaaca ttaaaacaac aaaagatatc tttggtaaaa
     8101 atgatataga agtatgggga gagaatacag gaaaattatt tagagatgat tatagagaag
     8161 gagtatctag taaaaaaaga tttttacaaa atctttttga atatgaagaa acatttttta
     8221 tggttgaaaa atattttttg tatgatgaag acgaactttt aggtactata gggatagtag
     8281 acaatataat atatagtggt tacagtaata gaaattataa ctcaaaagat ttaatgaaga
     8341 tgatagaaca ctcaattcca gagaatatgt tttataaaga tgtgtatgga aattatattg
     8401 gatttaattc aggttttttg aatttagctt gtatgaataa agaagaatta ttagggaaga
     8461 atagttataa aatatcagaa gaagaagctt taatagataa gatatttgaa agtgataagg
     8521 gtgtagttga aaataaaaaa gtagttacat ttgagttaaa tataagtatg aatgatgaaa
     8581 acaagtgtat tgagattaca aaaaggccgt tttttgatag ctatggaagt gttattggaa
     8641 taattggaac agcaagggat ataagtagaa gaaaaagatt agaagaggaa atggataaaa
     8701 ctaggatgga attttttgca aatttatccc atgaacttag aacgcctata aatttaatat
     8761 catcttcatt acaagtgata gaaaaaaaag aagcagattt aatagaatca aatgatactt
     8821 taaaaagaaa tctaggtata ataaaacaaa atggaaatag aatattgagg ttagtaaata
     8881 atgttataga ctttacaaaa atgcaatcag gatatttaga ttttaaacca gaagaatctg
     8941 atataattgc atttatagag gagatatgta tgtctgtagc tgattttgca agccaaaata
     9001 atatacaact tacatttgat acagaaatag aagaattttc aatgttattt gattcagaaa
     9061 aattggaaag aataatatta aatttacttt ctaatggaat taagtacaat aagaaagatg
     9121 gaaaaataaa tatattttta tatgtgaaag ataatgtatt taatatgaaa atatctgata
     9181 gtggaatagg aataccaaaa gaaaaaatag ataaaatatt taataggttt gaacagattg
     9241 acaatgaact ttcttacaga gttaaaggaa gtggtattgg actttcatta gtgaaatctt
     9301 tggttgagtt gcatgaggga agtatatctt taaaaagtca acttggtatt ggaagtgaat
     9361 ttatagtttc attgcctgtt agaagtaaaa ataatattga aaaatataat cataaaagag
     9421 aaatcagcaa tgaactaagt aaaaaattag aaatagagtt ttcggattta taagtttaaa
     9481 tataaaaaat aaaattttaa aaaagtattg acaattgatt tagaagggtt taaaataaat
     9541 tacaacataa taaataaaaa ggcgatgaat aggaagagta aatctaattc gattaccaga
     9601 gagaagagtc cattggctgg aagacttttt aaattagagg gattgaaaac tacctaggag
     9661 ctactttctg aagtcatctg cattagtaga ttttagtaag aatagtcgtt agctgcgtta
     9721 aagtgtaaag tgagttttca tgacatatga aaactaaatt aggtggaacc gcgggaataa
     9781 tactctcgtc cttatgttat aaggatgaga gttttttgtt atttaaaaat ataaacagta
     9841 atcataataa ttatagaaaa tataaaagcg atgaatagga agagtaaatt taattcgact
     9901 atcagagagg aaagttcaca ggctgaaaga ctttctaagt ttgagaaatt gaaaactacc
     9961 taggagctgt ggtctgaagt tactttaaaa agttttagta agactaaccg ttaactgcgt
    10021 taaagtgtaa agtggatttt atcaataaat gataaaatca aattgggtgg aaccgcgagt
    10081 atggactcgt ccctttatat agggatgagt ctatttttat tgcaataaaa gattgaaaat
    10141 aaaaaaaaat cggaaaatta taaatttagg aggaaaataa aatggagaat aaagtgaaaa
    10201 ttggagtact aggatatgga gttgtaggaa gtggacttat agatataata gataataata
    10261 aagaaaaaag aaatatagaa atagttggaa ttttagttaa taatttggaa aaacataagg
    10321 ataaaaaata ttctaatata ataactaata atatagatga tatatttaat aaagatatag
    10381 atatacttgt tgaagtcatg ggtggtttag aaccatcatt atcttatata aaaaaagcat
    10441 taaacaataa aattcatgtt gttacagcaa ataaagattt gttagctgaa tgtggagatg
    10501 agcttgcaaa attagcaagt gaaaataaag taagtattaa atttgaagct tcagttgcag
    10561 gaggtatacc agtattaaaa ccaataatag aatcactaga aggaaataat atagatagta
    10621 taaatgcaat tttaaatgga actactaatt ttatattatc taaaatgtat gatgagaatc
    10681 tatcttatga tatggcatta agacaagcac aagaattagg ttttgctgaa gcaaatccag
    10741 agtcagatgt attagggtat gatgctgcta gaaaactatc aatattatca actctagctt
    10801 atgacaatag agtatattgg aaagatgtgt atttagaagg aattacagat atagatgaaa
    10861 aagatattga atatgcaaaa aaattaaatt gtaaaataaa attgattggt caaagtaagt
    10921 atgaaaatga taaagtaagt gcttttgtta gacctgtttt agtagaaaaa gataatatcc
    10981 ttgctagaat agacaatgag ttcaatgctg tcatagtgaa tggagattca gtaggagaag
    11041 tttcttttgt aggtaaagga gctggaagtt tagcaacagg aagtgcggtt tattcagatg
    11101 ttatagatat aatagataat agagtttcaa gtatagattc ttttactaaa gataaaatac
    11161 aagttaataa aatagttaga gaaaagtgtg gagcacttct tagatttaaa aaatgtaata
    11221 aagatgaaat tctaaatata gttgagaatt gtttagtaaa attcgatatt ttaaatgatg
    11281 atgatgaact tgctattatg gtttatgctg attctgaata tgaaataaac aacagtttat
    11341 gtctaataaa agataaagga tactgcgaaa aaatgaataa aatgttgaaa ataagttaaa
    11401 aaagaaaaaa tataagttta tatgtaacat attttgacga ttttaatact atatagtatg
    11461 agatgaaaca aaacatctca taaaaaaatt aaaattaaag ggagagatat gttatatgga
    11521 aaataaaaaa tgttattcag aagattggta tgaaagagga gaatctacag ctaaatggtt
    11581 ccaaaatgat agagaagaat atgaaagaga agcatatgat gaagatagag aaagaagagg
    11641 ttcaaactgt ggatgttcag attcaggaga aaatagacct agaaactgtg aaagatttag
    11701 aagagaagct gagataagag aaagagaagc aagagaagca ttttgtgaat cttcagagaa
    11761 aaagaaagag gcattagcat atgaatgtga agctagaaaa ttatgggaag aagcagaaaa
    11821 atactgggat gaatattcaa aatacaacta taaaggaatc gaatatttag cagaagctgc
    11881 tagattattt gatgaaggta tggaatgtga agctagaaga aatggaaata atggaggaaa
    11941 caataataat tgttgccata aatgccataa atgtaattgt aactgctgta gaaaataaaa
    12001 aaattaattg acaaatcaat agttaaaata tatcatatta tgtataataa aaagaaaatt
    12061 cagaaaaaaa taatgattag aagaaatata agtatattta ttagacaaat agtgctcagt
    12121 tagtgaggtg agtacaatat gattaaactc ccatcctcta gcttttgata gagggtggga
    12181 gtttttattt attgatatat cttaaatgag gataatattg taaaataaat gagtatgaga
    12241 ttgtaaaatt aggaggtaaa aaatgaaaag tattaaaaca tcaagtatga aagaaaaggt
    12301 agatgaaaag atattaagag agcaagtaag ttctataata gaagatataa gaaataataa
    12361 ggatgttgca cttaaaaaat ataatgagaa gtttgataga aatacaagag atgaatttag
    12421 aattactaag gaagaaataa aggaagctta taagcatgta gatgacgaat ttataaataa
    12481 cttaaagatt gctgccaaaa atataagaga atttgctaaa gctcaaaaac taagttttga
    12541 aaaccctttt gaaaaagaaa tttatccagg tgtaatctta ggtcaaacta atattccaat
    12601 agagtcatgt cttgcatatg ttccaggagg tcagtatcca ttgttttcaa cagctcttat
    12661 gcttataata cctgcaaaag tagcaggagt aaaaagaata gtggcatgtt caccaactat
    12721 gaaaaatact gaaaagataa atcctaagac attggtagct atggatatag caggtgctga
    12781 tgaaatatat gcaactggag gagttcaagc tatagcagca tttacatatg gaacagaaaa
    12841 gattaatcca gttgacataa ttgtaggacc aggaaataag tttgtaactg aagctaaaag
    12901 acaatgttat ggacaagtag ggattgattt tgtcgctggg ccaagtgaag ttcttataat
    12961 agcggatgaa acttcaaatc cagtttatat tgcagctgat ttattagcac aatgtgagca
    13021 tgatttaaat gcaagaggaa tattattgac aaatagcttg gaagtagcac aaaaagttga
    13081 aaaaaatata gaaagtatgc taaaagattt gccaacaaag gacatagcat atagttcatg
    13141 ggaaaataat ggagagataa tattggttga tgatatggaa gaagccatta aaataagtaa
    13201 tttttatgca ccagaacatt tagaagttgc agtaaatgag tataatgata tttgtgatag
    13261 attaactaac tatggttctt tatttattgg aaatctttct gctgaggtgt ttggtgacta
    13321 tgtttcaggt accaatcata cattaccaac acttaaagca tcaagatata ctggtggtgt
    13381 ttgggttgga acttttatca aaacttgtac aaagcagata tttaatgaaa aagctataca
    13441 atcattagca cctgtagctg aaaagctggc aaaagaagag ggattatatg cacatgcaaa
    13501 ggctgctgag gttaggttta aaaagtagta aaaaatttta ttttggcagt cgagacttag
    13561 tattagactg agataaggaa aacgctgaga acctgaataa atactatatt ttgtgggtct
    13621 cggcgcttct tttatatctg tatataatag aataatacta tctgtgtgtt taaatactaa
    13681 cttaatattt cttttctaaa taatgtttat gaaatagtta atatttttag ttaatacttg
    13741 aaaaaaactt ttgctaactg tgcaaattca tctgcatttg tcaatatagc agggtgtcct
    13801 ccctgactaa aaatgtaaat acttgctttt gaaataaaag ttgccatttg cttgtactct
    13861 tcttcaagat tatgacgaca catttcgtct tccttacttc ctgtaaataa aattggcatt
    13921 tctaatttac acaattcttt atgaaacaaa gggcgtttct cgtttgcaca ttgcaataga
    13981 gcttttgtat caagattaac aacattctcc caatcttttc catgacacca ttcatagaat
    14041 tttcgtgctt gaatatcttg ctttgatttt tcccttccag tgattagatt atctatgaag
    14101 ttatcattaa gagtacgtcc atcaaagcta tctgctataa ttgcttcaac taaatcggga
    14161 cgttctaatc cagtattaac agctgcccat gctcctccac ttgttccaat taagccaact
    14221 ttaccacaat tcaaatgttc aatcagtgct attgtctgta aggcttcatc atgccatatg
    14281 tcaggagaaa attgagaaac tctatccgac tgtccattac ctaaaaaatc aattaagata
    14341 catctaaaat tttctgcata taatggcaca agtgctttaa acatattaga agaaccagta
    14401 ttaccatgca ggaaaattat cggtttacct tgtccatatt ctttataaaa acaagaatta
    14461 ccttgatact gaaaatatga catatatttt cctccttttt atttgttaag ttaattataa
    14521 caatataggt atgaatgaaa aacctcatgt tctttcttag aataaaaatg tgccacgcat
    14581 aaaacgtgac acatttctaa ttataaattt tgctgtttaa gtattctttg tatgcttttc
    14641 tctgtcaaaa agtatttctc tgataactgc ttgatactca ttccattttg gaaatttata
    14701 cagatttgag aattacgaga agctaagact tgtttggtgt ttgtgttttc accccaatgc
    14761 tttttgtgct cttgcttttt gggaatataa atcactttac catctatata ttcttgcact
    14821 tgctcaatca gttctttagg cagtaagtca atcgaatttt gatagctcat tttgcacctc
    14881 ctaaataatt attctttagg attagcaaag gctactgcca ctcacaatta aaaataaaat
    14941 tttttattat gtacaatagc ctttgctatg cacaagttcc aattcccata aactaaatac
    15001 tcctttctgg tttgaaaaat tcatctgaac attgatttcg cttttaagat agagttcaat
    15061 gacaaattta catttttatt atatatattc agatagataa ttgcaactgt ctttgtatga
    15121 ggatatttta ttatagtcaa tactataata tgtatttata tcatcaaact aatatgaaca
    15181 gttgaaagga atatgaacaa taaaacttag tcaggaagaa taattatgaa gcagacaata
    15241 gagaaatttg attttaaagt tttattgaaa atttattgac aaatttaact tgtaaatatt
    15301 atactaaact taataaataa agtttataaa gtaaggtgtt gtctatgaaa aatatagttg
    15361 gaaaaccgca aacgataaaa aaagtaaatg aagatttaat aaaaaacatt ataaaaaatg
    15421 aaggtcctat aacaaaacca gaaatttcaa gcattactga attgagttta gctacagtta
    15481 ataagattgt agagcaatta gctttgaaaa atgaagttaa agtaagtgga ctaagtgaat
    15541 ctactggagg aagaagagct caattatttg aaataaatgc aaacttagaa cacataatag
    15601 ctttatatta ttatagaaat tgttgtatag gggtagttgc caatttatta ggtgaaataa
    15661 tttatcaaga agaatttaat ataagaatgg atacgtatga aaacgttaac caagatactt
    15721 tttttgttat agatactcta ataaaaaagg aaaaaggaaa caatataagg gctattggaa
    15781 ttggtgtccc aggagttgta aaaaaaggtg tagttaaaaa tataccaact ataaaaagtt
    15841 gggaaggtat aaatttagaa aatttaataa agagtaaata caatactaaa gtgtttatag
    15901 aaaatgatac aaatattact actattggga tttatcaaaa acagtataaa gacaaatata
    15961 aaaatatggc tcttatgtat ttagaagatg gtattggttc aggtatagtt ataaatggtg
    16021 aattgtattt aggttctaca aattttgctg gagaattaag ttatctaaat atagaatttg
    16081 gtaagtataa ctctaacaac ttaaatcttg aagattatat tatgagttta agaaaaagat
    16141 atttagaaag taaagataat aaatttaaga aagaaatttt atctataata tcaaacttta
    16201 taaggactct tgtatgtgtc ctaaatccac aagctatgat tatacagtgt agtatattaa
    16261 ccaagagtga catggagttt atagaaaaag atgtaagcaa taatgttgga gaagaaaatt
    16321 ttcctaaatt aataaaagta gactcattga gagaaggaag tataaatgga gtaattagta
    16381 tgtgtttaaa agaaactgac tatcaatatt ccttatctaa taaaaaagga ggatattaat
    16441 gtgaaatatt tggtttcaat agatggaggg ggaacaaaaa ctaaattttg tgtaagtgac
    16501 ttagatggaa acatacttaa agaacatacc actggttcaa ctaattataa gtcagttgga
    16561 attaaaaaaa catatgaaaa tattaataat ggatttaaaa aaatattaaa agatttatat
    16621 atcgattatg acgatattga gtacactgta tttggtatat ctggttgtga ttctcctaat
    16681 gattataaga ttataatgga tgaaatatta aaaattggga ttaacaaaga aaaaatatat
    16741 ctggcaaatg atgcagtttt agctttttat gcacaagctg attcccctgg cttagtaatt
    16801 gtggctggga caggttcaat tatactggga attaaagaag atggagaaat atatagagtt
    16861 ggtggatggg ggtataattt tagtgatttg ggttctggat atgatatagg gagaaaatta
    16921 ttaaagaaag tattgcttta ttgtgatgaa tgtcatgagt atagtgattt atttagttgt
    16981 gtcttggact tctttggagc aaatagtttt gaacaacttg catatatgat tactgatata
    17041 aatgataatg ttgaaatagc aaacttagct tcattagtga ttgattgtgc cgaacaaggt
    17101 gataaattgg ctattgaaat attaagagaa tcatcaactg aactatctaa attagctcaa
    17161 gtaatattga gaaaaatatc taatccaatg aaaatggata aaagcgaaat aaatatagtt
    17221 ttgtctggtg gtacattaaa taaaactgta tatgctcaaa tgttaatgaa taatttaaaa
    17281 acagaaaata cagataaaaa tttaaaattt atatctcaag aaaatcagcc agtatatggg
    17341 gggataagac ttgctatggc attagctaat aggagggtca agcatggata gaaaaaaggt
    17401 aatagtacaa atttttactg gaggttttaa ggctaaagaa ataaagttta atgatataaa
    17461 atgtaaacta ctagaattat tggactgtat tgatatagaa aaggttatca tgggatggag
    17521 tgtagatgaa gaattatatg tagaaactaa aaaactatta aataaatata atgttgaact
    17581 atatctttgg ttgccatgtt tttctgaaat aggattactt gaaaaaagta atttactaat
    17641 tgattatatg gggaaggaag tcaagggcta tgctttacaa gaggatgaaa attttgagtt
    17701 ttattgccca aataataagg aaaatataaa aaatataaag aaaatatata gtacttattt
    17761 tagtgatatt aaatttgatg gaattttttt agataaaata aggtatggtt ctttttcgaa
    17821 taaactaaat ggtgttttta attgtttttg taaagattgt ttaaatatat ataaaaaaaa
    17881 taatattgat aaagagtcgt taatgagaga aatgaataat gtgtctaagg gagttataga
    17941 taatgaagat actccatttg gtataacaga atacattaat ggaaaataca aatttaaaaa
    18001 taaaatatgg gaagattttt ttgaaacaaa aaatcaaaat atatacaagg cattattaga
    18061 gataagtagt tattttaaaa gtaagggatt gaaaataggt atagatgctt tttctccttt
    18121 tatttcttac tttacaggtc aagatttaga aaaattacaa gaagtagcag attttataaa
    18181 accaatgatg tatagaacta caaaagctcc agctggttta ccatttgaat ttgaaaggtt
    18241 tattgatgaa tccatacata agaataaaga gattggaaaa agaaatttct tagatatttt
    18301 aggaattaag gagtatgaag gagatgtata tcctatagaa tttgtactag aggaaatcga
    18361 ttttatgaca agaaatctta ataaagatat atatgtagga gttgaaataa ataaaaagga
    18421 aattatatct cctgttacac ctaagtacat aagtgaaaat atttcaaata taaataagac
    18481 aacagtaaaa ggatatgttt tatcttggga tttgttgtca gcaccaaaag aaaatattga
    18541 tgaaattata aaatggtttc ggaattagtg aggtgataaa gtgagtaaga tattggaaat
    18601 acaacaaata tataaaagtt ttggaaaggt agatgtttta aaagggataa attttgagtt
    18661 agaaaaggga gaagtaagag ctcttttggg agctaatgga gcaggaaaat ctacacttat
    18721 taaaatttta ggaggagttc attctcaaac taaaggtaaa gtaattttga ctggtaatga
    18781 aataatgttt aaaaatccac atcattctaa agaaaatggt attagtataa tttatcaaga
    18841 attaagcttg ataccaacgc ttacagtact tgaaaatatt tttcttggta gagaaatttt
    18901 aaaaggaagt gtgtttttaa acaagaaaga catgcttgag gaatatatga aaatttgtga
    18961 ggattttgat tttaatatag acccacatat agtagtttct aatttaagtg tagcaaacca
    19021 acaaatggtt gagataatga aagctgtttc atgtaattct gaaattatca taatggatga
    19081 acctacaaca tcacttacta ataatgaaaa agatgggcta tttaaaataa taagtagact
    19141 aaagtctaaa ggtaaaacaa tagtctatat ttctcacata ttagaggaag tttttaaagt
    19201 atgtgatagt gcaagtataa tgaaaaatgg aattatgatt ggaacttatg atgtagagga
    19261 tttaactaaa ttaaagataa ctcaacttat gacaggaaat gataatcaca aacttattga
    19321 gaagaaggat tatacatttg caaaatatga agagccacca gtactagaag taaagaatat
    19381 aagctggtct aacaaaataa aaaatgtttc atttaaagtt tatagaggcg aagttgtagg
    19441 tcttgcaggt cttgtaggtt caagaagaac tgaaattata aatttgatat ttggtataga
    19501 taataaagat agtggagata tttttatcaa tggtaaaaaa gtagacataa cttctcctaa
    19561 ggttgctata aaaaacaaaa taggattgat tcctgaagat agaaaaaatc taggattaat
    19621 tttagaacaa gctatataca caaactcatc atcagtacaa attgataaat tcaagaaatt
    19681 tttgtttata gatagaaata aagaaattga gttttctaaa aaaggtgtta aggaattagg
    19741 aataaaagtt agtgatgtaa agcaagaagt aaaagagctt agtggtggaa atcaacaaaa
    19801 agtagtagtt tctaagtggt tagagaaaga tttagattta ataatatatg atgagccgac
    19861 aaagggaata gatattgctg caaaagaaga tattttcaat acaatcaaag aattttcaag
    19921 taaaggaata ggagtaatat ttatatcttc tgatttagaa gaagtaataa gagtaagtga
    19981 tagaatttta gtaactagag aaggttctat tgtagatgat ttaaaaaatg agaatataca
    20041 ggttcaagat ataatgaaca gtatatttaa tgtataaaaa atgaaaatgg aggactgaga
    20101 tgaagaaagc ttttgagaag ttaaatttaa gagattatgg agtattatta ggttttttag
    20161 ctttatgtat agctataagt attgcaactc catcattttt aggtaaacaa aatatactta
    20221 atcttttacg acaatcttct ataattggaa taatttcagc aggtatgact tttgttataa
    20281 tatctggaaa ctttgatata tcagttggag cagtagcagc tcttgcaggt gctataacaa
    20341 tgaaatttgc gactggtgga actaatttgt ttgcatccat gttcttaggt gtagcgattt
    20401 gtgcagtaat aggattaatc aatggtatag tagttgcaaa gataaatgta ccatcattaa
    20461 tagccactat ggctatggtc actattgtaa gaggattgct gttgatgctt acaggaggat
    20521 atccaattac tgaaaatata ccaatacttg attacattgg aaatggatat ttgtttaata
    20581 taccaatacc agtagtaatt ttctttatag tggtgttagc ttcgtttatt gtacttaata
    20641 aaactaagtt tggtagatat gtttattctg taggagggaa tcaagaagca agtaaactta
    20701 atggtataaa tgtagattct cacaaagtga aagtgtttat tattaatgct gtacttgcag
    20761 gaatagctgg aatcgtacta acaggaagac ttgggactgc aactgcgatt gcaggtgaag
    20821 gatatgatat ggatgcaatt gcatctgtag taataggagg tacatcagtg gcaggaggtt
    20881 ctggttctgt acttaaaact gttataggag tattacttat gagtgttata aacaacagtt
    20941 ttaatcttct tggaatagat gtatatttcc agtacatatt taaaggatta ataattttag
    21001 cagcagttgg atttgattct tatagcaaga aaaaacttgc ttcaagataa aaaataaggg
    21061 ggattttttt atgaaaaaat taatgaaaca tttagcattg ttgctatcac ttgttatgat
    21121 atttggttta gttggttgtt caaatggtgg tgacaaagaa aaaaatggag aaaagaaaat
    21181 agcagtatta ctaccgggtt caacaggtta ctttgtagca actaaacaag gtatagatgc
    21241 aaaatctaaa gaattaggtg taagcgtgga atatgcagat gctcaatggg atgctagtaa
    21301 acaattatca caagctgaag attttatggc taaaggggta gacatgattg cattatgtgg
    21361 agttgattct gctgtaagtg agagaatagt taaagctgct aatgattcag acgttcctat
    21421 agtagcattt acaaattcta taggaagtaa cccaactgga gaatttaaag gtttagttac
    21481 atatatagga caaaatgagg aagaaacagg agcattaact ggaaaaattg ctaaaaactt
    21541 attaggagaa actggtggga aagctgtatt aatagaagga gtaccaggaa ctactccaca
    21601 agtattaaga aaaaaaggtc ttgaaaaaga attaaaagat agtaatatag aaatagttta
    21661 taatcaaact tcaaattggg agaaagagca agctttaaag gtaacagaag atttaattca
    21721 aaagaaaact gattttaatg ttataatatg tcaagatgat aattctgcta caggtgcagg
    21781 tcaagcttta aaagatgctg gtcttaagga taaagttaaa gtcataggtt tgggtggaag
    21841 taaagatgga ttaaaagcta taactgatgg gttaatagat ggtacaactt atatgtctgc
    21901 tgtagaagaa ggtggattag tcatagaaaa ggcatctaaa ttccttaaag gtgaaaaaat
    21961 agaacctgtt acacaaatta aacaagttga agttaacaaa gataatattt cagagttcaa
    22021 gggtgaatgg taaattgtca tagataataa ataagatttt tagagttact ttagaaacta
    22081 tataaagtaa ctcttttttt aatttaattt agtgataaat tattgaatag tatttctaaa
    22141 caaataaaaa attaatataa acaaagactt aattatacta aattataagt aaaactaata
    22201 taaacttatg gaaaaaagga aaacgtctaa agtattggaa ttactatagt gtaggtgtgg
    22261 tattaaaaaa acataaaaaa acataaaaaa attgttgaaa aaaatataga aattttgtat
    22321 aattaaagat ttgaaaagtg cggtatacaa agccaattct tttgtatata atatatataa
    22381 attcagaaaa aggaagggat atgtatggcc aggcggttat ttttagaaag gattggactt
    22441 atatttttag gttcagcaat attagcattt ggagtttata atttttatta tttaaacaac
    22501 ataacagaag gtggagtgtt aggtatttta ctactgttaa aaaatttatt caatatacaa
    22561 ccagcaatag caaatgttgt aatagatagt ttactacttt tagtaggata taaatttttc
    22621 ggtaaaaaat tccttatata ttcaatagta gcttctataa ctttttcagt gttatatgac
    22681 ttatttgaag ctataggacc tctagtacca caatcacaaa atatgctttt atcaactatt
    22741 ttggcaggtg taactgttgg aactggtgta ggtatagtag taaaagcagg ttgtgcatca
    22801 ggaggagacg atgctttagc attagtaatt tctaagacta catctttgaa catagggcaa
    22861 gtttatttag caacagatgt tattgtattg ttgttatcat tattttatct atcagcgttt
    22921 gatatctttt attctttgat tgctgttaca attagtggca aggtaattga ttttatttat
    22981 tatcatggta aaagtttgga tatggatata agtaatgata tagttccaga atgttaatat
    23041 aaattaaata actttagatt ttattagggg tggcagattt gccacctttt ttttgtctaa
    23101 atctttatag aaagtataat cccatctagg tttttgcaaa tctcaaatat agaattatgt
    23161 tggaatgact gggaatacga ttgctagatg tgcattttaa aaacccaatt ttatatttat
    23221 gaaatactaa tcattaatgg atgtttctta atcctatctt tatacctaaa atgataagat
    23281 aaatgaagta gcaatacaaa gctaaaattt gtaaaagaga ttaaattaaa tggaggagct
    23341 aaaatgttgg atatagttat gatagcattg ttattcatag gatttgtttc catgaagtta
    23401 cttgctgatt ggtgtgggaa acaaactgaa aaaaaataat gtaatgtatg gagggattac
    23461 ctatgtggtt tctggcagtt gtaattataa tgttaataat atatttggta tatgcgctta
    23521 ttaatcctga aaaattttaa acagtataag gctaataagt aagttgaatt ggggggaaca
    23581 atttttatgg tgcagatagt aatagtgtta gcgattttta tgattttagt aatacctatg
    23641 ggcaagtatt tatatcatat agctactaat caaaaaacat ttggagatag actatttgat
    23701 aaagtggaca attttatata taaggtatgt agtatagata agaaaaaaga aatgaactgg
    23761 aaacagtatg cattagcttt attatttaca aatgcagtta tggtatttat aggatatatc
    23821 atacttagga ctcaaagtat gcatattttt aatccaagtg gaattaaaag tatggaacaa
    23881 ggactatcat ttaatacaat aataagtttt atgacaaata caaatcttca acattattca
    23941 ggtgaatcag gactttcata ctttagtcag atgacagtga taatatatat gatgtttaca
    24001 tcagctgcaa caggatatgc ggctgctatg gcatttgtaa gaggtctagt cggtaaaaag
    24061 aaaacattgg gaaattttta tgtagattta attaggataa caacaagagt acttttacca
    24121 ggagctttaa ttattgggct tatattagtt acacaaggag taccacaaac ttttgctggg
    24181 acagaaacag tgactactat agaaggaaag cttcaagata tagctagagg tcctgtggca
    24241 gctcttgaat caataaaaca tttaggaaca aatggtggtg gattctttgg ttcaaattca
    24301 tcacacccat ttgaaaatcc aacaataata tcaaatatag tagaaatatt atctatgatg
    24361 atattaccag gagcttgtgt tgttgcattc ggtcacatga taaagaataa aaaacaaggt
    24421 tgggttgtgt ttggcgcaat gtcaatcata tttttaatag gtctagtagt gtgctttaaa
    24481 gcagaaagtg caggaaatcc tattttatca caattagggc ttaatcaaag tatgggaagt
    24541 atggaaggta aggaagttag atttggtata gcacaatcat cattattcac aacagtgact
    24601 acttccttta cgacaggtac agttaataat atgcatgata cactgactcc tttaggagga
    24661 ttagtaccac ttttaaatat gatgctaaat gtagtatttg gtggtaaagg cgtaggtctg
    24721 atgaatatgt tgatgtacgc tataatagca gtattcttat gtggattaat ggtaggaaga
    24781 acaccagagt ttttaactaa gaaaatcgaa ggaaaagaaa tgaagcttat tgctttactt
    24841 attatattac atccactttt aatactaatg ttctctggtc tttcagtagc aataccagca
    24901 ggacttgaag gaatttcaaa tcctggattc catggattat cacaggtatt gtatgagttt
    24961 gcatcttcag cagccaataa tggttcagga tttgaaggtc taggagataa cactatgttt
    25021 tggaatataa caacaggtat agttatgttc tttggaagat atgtgtctat tatagtacta
    25081 ctagcaatat caagtttgtt agcatctaaa aaagctgtaa atgaaagtat aggaacactt
    25141 agaacggata attttacatt tacaatagtt ttagtacttg ttgttttaat tgttggtgct
    25201 ttaacatttt tcccagcctt agcgcttgga cctatttcgg agcatctagt gttgtggcac
    25261 taaaaaattg ggagggagta aagttttatg gaaactaaaa aaacaaagtt tattacaaaa
    25321 gatattttca aaacttccat tatagaagct tttaaaaaat taaatcctaa atatatgatg
    25381 aaaaatccag ttatgtttgt tgtagaaatt ggatttatag taacattgat acttacaata
    25441 gtacctagtt tgtttggtga tgaaggcaat aacttaagag cttataatgg gattgtaaca
    25501 ataatcttat ttataacagt cctttttgca aattttgctg aagctgtagc tgaaggaaga
    25561 ggtaaagctc aagcagatac acttaagaaa actagaaaag atacagtagc aagggttata
    25621 gatacgttag gaaatgaaaa aatgataagt gcaagtgagc taaaacttgg tgatattgtt
    25681 ttagtaaatg ctggagaagt tatcccaaat gatggagaag ttatagaggg tatagcttct
    25741 gtggatgagt cagcaataac tggagaatct gcacctgtca tgagagaatc aggtggagat
    25801 ttcgcatctg ttacaggtgg tactacagtt gtaagtgact ggttaaagat aagaataaca
    25861 acaaaaccag gagaatcgtt cttagataaa atgatagctc ttgtagaagg tgcttcaaga
    25921 caaaaaacgc caaatgagat tgctttaaat actttgttag tagggttaac acttatattt
    25981 atggttgttt taatatctct ttatccaatg gcaaactatg caggtgtaaa gattccaatt
    26041 tctactatga tagctctttt ggtatgttta ataccaacta ctataggtgg attgttgtca
    26101 gctattggta ttgcaggtat ggatagagta actagattta atgtaattgc aatgtcagga
    26161 aaagcagtag aagcttgtgg agacgttgat actatgattt tggataagac aggtacaatt
    26221 acttatggga atagacttgc atcagaattt atagcagttg gaaatgcaga ttcaaaagat
    26281 ttaataaatt attctgttat gtgttcatta aaagatgata caccagaagg aaaatctata
    26341 gttgatttag gtatgaaatt aggttcaaca ggtaagacaa aagaggcaga ggaagctgag
    26401 tttgtagaat tttctgcaca aactagaatg agtggtatta acttagctga tggtagagct
    26461 ataagaaaag gtgcttatga tagcataata aaaagagtaa aagaatctgg tggtacagtg
    26521 ccagaagatt tagaagaaaa tgtaaatcga gtagctaaac taggagggac acctttagta
    26581 gtgtgtgtag ataatgagat atatggaatt atctacttaa aagatacagt aaaatcaggg
    26641 cttgtagaaa gatttgctag acttcgagag ataggaataa agacaataat gtgtacaggg
    26701 gataatccct tgacagcagc tacaattgca agagaagctg gagtagatgg atttattgca
    26761 gaatgtaaac cagaagataa aatagatgca ataaaaaaag aacaattaga gggtaaaata
    26821 gttgcaatga caggtgatgg aacaaatgat gctcctgcac ttgctcaggc agatgtaggt
    26881 cttgcaatga atagtggtac aacatctgca aaagaagcag caaatatggt agatttagat
    26941 tcagacccaa ctaagatatt ggaagttgtt gaaataggaa aacagctttt aataactcgt
    27001 ggggctttga caacatttag tatagcaaat gatgttgcaa aatattttgc gattatacca
    27061 gctatattta ctcttgcaat acctcaaatg gagatattaa atgtaatgaa actttctaca
    27121 tcatttagtg cgatattatc agctttaata tttaatgcta taattatccc atgtttaata
    27181 cctcttgcaa tgaagggagt aaaatataga cctatgaaat cacaagctat gttaatgaga
    27241 aatatgctta tatttggtct tggtggagtt atagtaccat ttttaggaat aaaattaatt
    27301 gatttattga ttacaccttt agtaatggtt cttaatttag gttaataaat ggaggatata
    27361 aaaatgaaaa cattaaagcc agcattactt gtaagtattg tattgttagt tgtttgtgga
    27421 cttgtatatc cacttgtttt gacaggtgta agccaagttg catttaaaga taaagccaat
    27481 ggaagtatga tagaagtaaa tggtgtaaaa gtaggttcag aacttattgg tcaaagcttt
    27541 acagatgtga gattctttaa gggaagagta tcatcagtaa actataatac ttatacaaaa
    27601 gaagatttag taccagataa agatggaaac acatcttatg gaggtgtatc atcagggtct
    27661 tttaattatg gagctacaaa tccagaactt catgatagag tacaaaaaga tatagaacag
    27721 tttttaaagg ataatccaac tgtcaaaaga gaggatatac caacagattt acttacagca
    27781 tcaggttcag gacttgaccc taatataagt ccagcctctg caaaaattca gataccagct
    27841 atagcaaaag cgtctggaat atcagaaagt aaattacaaa aaattgtaga tgataatact
    27901 tccaaaaaac tttttggagt tttaggtgaa gatagagtta atgtattaaa ggttaatgta
    27961 gaggtcgcaa aaatattagg tcttatatag tattttgtgg aattttagca acaaaatatt
    28021 acgcaagatt taatttaaag gttaaatttt aaagagtgaa aatcgataat gattttcact
    28081 tttttatttg aatattacta atatttggaa taaagtgatt gtaagtataa aatccaaaaa
    28141 aaggttgcat tatcaaaatg ataatactat aatatattta tcgttatcaa tttgataata
    28201 gatttggaca tataggagga aatagtatgt tatataataa cgcattagag ttgataggaa
    28261 acacacctgt agtaaggctg aataatttag gctgtactaa tgggtgttct aacatatatg
    28321 taaaattaga aaaagtaaat ccagcaggaa gtataaaaga tagagctgtt tatgaaatgt
    28381 tagagggctt agagcaaaga ggagaattaa aaaagggaga tgtactagtt gaagcaacta
    28441 gtggaaatac tggtattgca ttatctatga taggaaaatt aaaaggatat gaagtagtaa
    28501 tcgtaatgcc cgaaacaatg agcgttgaga gaagagattt aatgaaggct tatggagcaa
    28561 atttggtgct tacagatggt aaagctggaa tggcaggttc catagaaaaa gcaaatgaac
    28621 ttttaaaaga aaatccaaac tataaaagcc taaaacaatt tgaaaataaa gataatccaa
    28681 atgcacatta taaaacaaca gcagttgaaa ttatgaatga tgtcaaagat ttagatatat
    28741 tcgtatgtgg agttggaaca ggtggtactt taataggaac tgcaagatat ttaaaagaac
    28801 aaaatccaaa tataagagta attgcacttg aaccaaaaaa atctccagct atatcagaaa
    28861 acaagactgg acctcataaa atacaaggaa taggtgctaa ctttgttcct gaaaattatg
    28921 attctagtgt agttgatgaa gtaatattag tagatgatga agatgcattt gatacagtta
    28981 aattacttgc agcaaaagag ggaattttag tagggatatc ttctggagca aatatatttg
    29041 gtgctataga gatggcaaaa aagtatccag ataaaaagat agttacagta gctccagatg
    29101 gagttgataa gtatatgtct atgggaatat tttaataatt ttatttgagg gagtgaatac
    29161 tttgtttaag aaaataaata aagatattga atatataatg aagaatgatc cagcagctag
    29221 gtctaagata gaagtttttt tactttatcc atcagttcat gcaatgataa tgcataggat
    29281 ggctcatgct ctttataaga agaagaagct ttttacagca agattaatat cccagatatc
    29341 cagatttatg actggcatag aaatacatcc tggtgctaaa atgggggaag gtatattgat
    29401 agaccatgga atgggtgttg taattggaga gacagcggaa gtagggaata gggtgacaat
    29461 atatcaagga gctactttag gagctacagg aaaagatact ggaaagagac atccaactgt
    29521 tggtgatgat gtattaattg gtgctggaac aaaaatacta ggacctttaa atataggttc
    29581 aaattcaaaa attggtgcaa attctgttgt cgttaaggat gtacctaatg gtgcaacagt
    29641 tgttggtata ccagcaaaaa tagtaaaaat aagaaaccta gaacctgtaa aaaagaataa
    29701 aaaagaagtg tcctatgagt atgatgaatt agataatgta tattatatat aggatttata
    29761 taactataaa ataaacttta taaaaattat tttaaaaggt atttttttca aatttgagga
    29821 cataataaaa aagaactgaa tgttacagtt ttggttcatc aatctttgat aaacaagatt
    29881 ttaaatttgt agcgtctagg aggattttat tatgaaagca aatgttaatc aagatacttg
    29941 tataggttgt ggattatgtc catcaatatg tccagaagta tttgatatga aggatgatgg
    30001 gaaatcacat gttatagttg atgaagtacc aagtgatgct gaagaatcag cagcagaagc
    30061 tagagaaagc tgtccagttg atgcaataga tgtgcattaa taaaataaat gcctataaaa
    30121 attgaatttt caatttttat aggcatttgt cttttattca gttagtattt caacacctgt
    30181 ttctgtaact aaaaccatac attcccattg agcagataat ttaccatctt ttgtataaat
    30241 agtccaatta ttttcttcat caacaaaaat ttcgtgagtt ccttcattta ccataggttc
    30301 tattgtgaat atcatcccag gaaccaatag catcttagtc ccttttttag atacataact
    30361 tacaaaaggg tcttcatgaa agcttaatcc aactccatga ccacctattt ctctaacaac
    30421 agagtatcca tttttctttg cataagagtt gatagcatca cctatatctc ctaaaaatcc
    30481 ccatggtttg gcaacttcaa gacctttttg gacacattcc tttgtaactt gaactaattt
    30541 ttttgcattt tcattaacat tacctatcat aaacattctt gaagcatctg aatagtatcc
    30601 attaaaaata gtagaaacat ctacatttat aatatctcca tcttttaaga taatgtcctt
    30661 acttggaata ccatgacaaa cttcattatt tactgatgta catacacttt taggaaatcc
    30721 attatagtct agtggagcag ggatagctcc attttgtagt gtaaagtcat gaaccaactt
    30781 atttatatct tctgtagaca ttccaatttc gattttatct gaaatataat ccaaaatttt
    30841 tgtgtttatt acagcacttt tttttatttc ctcaatttga ctttcatttt ttataatatc
    30901 atgagttggt acaatatgac ctaacttagc aaatgaatct attttattat ccatatccat
    30961 atgacatttt ttatattttt tagtacttcc acaccaacag ctatcatttc tatttaactt
    31021 cataactttt catccttctt ttattattta tttaaattat gggttacata aactatttta
    31081 aaacataacc attataaata ttataacata aatactaaga aatcctttat agatagtgat
    31141 ttattcaaag tataaattat tatatggtta ttgttgatga aaagaggata gttaaatgta
    31201 ttattggata ttagcgcact taatagttta attcaagttt ttcaagaaaa tcatatgtta
    31261 gaggacaatt ttcctttaaa gtattttttg aatcttcatc aagataataa tgcacaaatg
    31321 attctgcaaa aaattcttct ataaacttgc ttatatatgc atgtgcaact tgcttagggt
    31381 cataaagctt atatctttcc tgagcaaata cttccataaa ttcttcagaa tttgattttt
    31441 tatttaaaac atttttatct atagcatgtg cagtttcatg taattctaaa ttttttgatg
    31501 aatgtacatt tgcatagcta gggttactat aacctatttt agccactata gaattatttc
    31561 tatatatacc aggtacatct ttccaagtct tgcctgatct tacccaacat ggaggtaatt
    31621 gatattttaa atcactaaat tctggttcat cagttagatt tgaatttatt aattttattt
    31681 ttatatcctt ttctttaagt gtctgtaaat atttaatatt tatttttgca agtctgtttt
    31741 tcatattttc aacttcatct ttatcataat tcaagtcatc aggaagtata agcatttgtt
    31801 caattatatc tatactactg tcttttatat tttcagattc ttcattaaat ttatattgaa
    31861 gagtattatt ttcatagata gttatatttg tattattttc tatagaataa gagtgaatat
    31921 cctctaaatc taaaatattc atttgttgta aaatatgttt tgtttcgcca ctccatacta
    31981 ctaaagaaag tgaattaaaa ttaattagtg gattttcttt tgcttttata tcattgctta
    32041 tatctacatc aaatacatat tcaattgagt cttctatgtt aagactatct gtattctttt
    32101 taatatataa atacgcttgt tttaagtcta tataaatctc atctaaagat tcgtcataaa
    32161 attctgataa attattagta aagagttctt cacttaaaag ttcattttta tcagttatac
    32221 tttcaattaa aatattaatt tcttttatca tatcgtaaat acttattttt ctatttgctt
    32281 tttcactaga tacattgcat ggcgcaagag caatgcaata aaaatctttt tcttttttat
    32341 aaaaggcttt aagtgatttt gataaaagtg taaagtctac acctatctgt aagttgacct
    32401 taagtgagtc attttgtaat aattcagtat tatttctttt agctgtattt aaattgtttt
    32461 ccataatcat tgcccccttt ttattagggt atcataattt ttttatatgt gtcaatttgt
    32521 ttttttgaaa attataattt ttttgaaaat tttatttttt ataatttgtg aattttttat
    32581 tatagaaata aatatgttat tattatgtca aaatttagca ttagattcat gtagagtatg
    32641 ttttatacat atattataat taattctttg aagattgcat attcaaaata cacaccattt
    32701 tttttaattc tataatataa tatttatata tttaatgtta ggatgtgatt taaggtgtat
    32761 tttacatata ttataagatg taaagatgat tctctataca ctggatatac cagtaatata
    32821 gttagaagaa tgaatgaaca taaattagga attaattcaa aatacacaag agcaaaggga
    32881 tttgaaaaac tagaagttta ttttgtgacc aacacaaaaa gcaatgctat gaagttagaa
    32941 tactatataa aaaaattaac tagaaataaa aaattgtcta taataaaaaa cccaagtata
    33001 cttataaatt taattgataa taaagaagat tatattattg gtaaggaaat agaacaatta
    33061 acataaatat ttatagtaac attataatgg gtgcatatgg tgtaaaaact tgacaataaa
    33121 tactattgta atataatgaa gttataaata tagtaaatta aaatagctag gggagccaag
    33181 agctgagagg tataccgacc cttacacctg atctggataa taccagcgta ggaaagctta
    33241 gtaaaaattc aagtatgtat tatttgtgat atatgctatt tctaagctat acctattgta
    33301 ggtatagctt ttttaattaa gttatttaaa ttaaacttta ggaggcaaca atgagtaatt
    33361 ataaaatacc aacacttaca atagccggct ctgattcatc tggaggagct gggattcagg
    33421 ctgatttgaa aacgtttagt gcaattggta cttatggtat gagtgtaata acagctataa
    33481 cagcacagaa tactaaagga gtttttgctg tagaggattt aaataagaaa ataataaaaa
    33541 aacaaataga ggcagtcttt gaagatatac ctccaagagc tgtaaaaata ggaatggttt
    33601 caagtcctga gattatactt gaaatagttg agaatctaaa gaaatacaat ccaaaatatt
    33661 tggttgtgga cccagtaatg atatctaaaa gtggatatta tctattaaag ccagaggcaa
    33721 aagaaaactt aattaagtat ttgataccac ttgcatacat aattacacca aatataccag
    33781 aggcagaaga aataacagga ataaaaatac ataatgtaga tgatatgaag agagtaggag
    33841 aagaaatact acaacttgga cctaaatttg tacttatgaa aggaggtcat ttagatggag
    33901 aagcagtgga tattttagtt ggtaaaaata tatttaaagt ttataaaagt gaaagaattg
    33961 ataaaaaaaa tacacatgga acagggtgca cattatcttc tgcaataaca tcttatctag
    34021 cattaggata tgaaataaca gaagcagtaa atctaagtaa aatctatata acagaagcaa
    34081 taaaaagaag ttttgatata ggtcatggag taggaccagt acatcatttt tataaatttg
    34141 agtaataaaa aaatattgga ggtagaatat gtataattta attaaagatg taaaaaaatt
    34201 aaatccattg gtaattcatt atactaataa tgtaactata aatgattgtg ctaatgtaac
    34261 tttagctgta ggagcaagtc ctcttatgag tttttcatat gaggaagtag aagaaatggt
    34321 aagtgtagca aactctgtag taataaatat aggaactatg aactcaaata tgttggattt
    34381 attcctatta gctggaaaag cagcaaataa gtacaataaa cctgttgttt tggacccagt
    34441 tggagtattt gcaagcaaag cgagagcaga acttacaagt agacttttaa atgaagtcaa
    34501 atttagtgtt gttaaaggga atgtatcaga aataaagttt ataggtggct ttaatgtaaa
    34561 gggtaaaggt gtagattcat ttgatgaaga agaagattct actgagataa taagaaagat
    34621 tgctgaaaag cttgaatgtg tagttgtagc aactggaaaa atagatataa taactaatgg
    34681 taaaggtaca tataaaataa acaatggaac tgataaatta aaaggaatta caggaactgg
    34741 atgtatgact gctagcttga ttgcaagttt tatggctgtt actgagaata tattagaagc
    34801 agcaacaatg ggtgttctta caatgtcttt aagtggggaa ttagctaatt taaataatcc
    34861 accaataggt acatttaaag aaaatcttat gaatgctatt tatcaaatgg atatagatac
    34921 tttaagtaaa aattctaata ttgaattttt aaattaataa agagtaaggg agacttaaaa
    34981 tgattgataa agaatcttta aaaaaatgtt taaagttgta tttagtaact gactctgaaa
    35041 tgcttaaagg tagagacttt tataaatgtc ttgaagatgc aatatcttct ggaataacaa
    35101 ctgtacaact tagagaaaaa aatgcttctg gaagagagtt tttaagaaaa gctatgaaac
    35161 ttagagaaat aactaaaaga tatggtgtta agtttataat aaatgataga gtggatatag
    35221 cacttatttg tgatgcagat ggagtacatg taggacagag tgacattgat gttagagagg
    35281 ttagaaagct tataggaaac aataaaattc taggagtatc agcaagaact ttagaagaag
    35341 ctatttgtgc aaaaaatgat ggcgcagact atttaggtgt agggtctata ttcacaacat
    35401 ctactaaatt agatgcaaaa tcagcttcgt ttgaaactgt taaagaaatt aaagaaaaag
    35461 tagacatgcc atttgtacta attggaggta taaatttaga caatatagat aaacttaaat
    35521 gtttagaaag tgacgggtat gctataatat cagctatatt aaaagctgaa gatatctcta
    35581 aagaagtgga aaagtggaca ttaaaaatat aaaattggct ctggtagtga aaaaaatgga
    35641 aaaatttgat ataatctaag agtaaaattt aagcaattat aaatgataaa cttttctaca
    35701 tatacttttt aatcaagata tatgtagatt tggttaaaaa ggagctataa atgattagga
    35761 tattgttagt gtgtgttgga ggcatgtctt ctaccctatt ggtaaataaa atggagaagg
    35821 atgcaaaaaa aagaaatata gactgtaaaa tatgggcagt tggagaagga gatataaaat
    35881 cggagcttga taactttgat attttacttc ttggaccaca acttagattt atgcttgatg
    35941 acgtaaagtc tatagttgga gatagagctc cagtatctat aatagatatg gtaaactatg
    36001 gcacttgtaa tggtcatgct gtgttaaatt cggttttaga aatattaaaa taaataataa
    36061 aaatggattc tatataatag agtccatttt tattatttgt tattaatagg cttaaaactt
    36121 agcttaccat ttcttttttt aatctccaca tagcaagtga aaaacttgaa cagcataata
    36181 ttaaaaatag taggataaca ggcattatat ctccaataag aatagtctca ccaaatatta
    36241 agctacgtaa agcgttaata acatgggtaa atggattgag catcactgct acttttaatc
    36301 ctccagataa actttctata gggaaaagag ctgaacttaa aaagaaaata ggaagtacaa
    36361 ttgcagtcat aattgtttca taaactattt catttggcaa taataaactt atagaataag
    36421 ctagacttga taaaaagaag gctgtcataa atatcagtac tatcataagt aggattccac
    36481 caatgccaga ttctatcctt actgagaaga atatacttac tatacacaaa atagttactt
    36541 ctatgaaaga aactaagata gcttctaata attgaccaag gacgattgaa tacctactga
    36601 taggtgcaat taagactcta tagaaacttc cactgttctt cattataaag tttataatac
    36661 caccactact acaagaagaa aacacaacca gtacaatgat acctggtagt ataaaagcag
    36721 tataattatt gatatttata ttattcatag tttggtttgc aatagaacta taaagcacaa
    36781 gccaaagaaa aggttgtaat atagtaacta caaatgagat tggattttta aaacgccact
    36841 tcatactacg ccataagata tttaacattc ccattcctcc tttaagcttt ttttagaact
    36901 tgtaagtgct aaaaatacat cttcgagact tggctctaca atttctattg catcaaattc
    36961 tatatcatgt tctaaaagcc atttatttat taaagtaaaa gcagttctac tatcgtttac
    37021 acttataaaa attgaatttt ctcgtacgct cataaacttt actaaaccag tacttttaat
    37081 tgcatcttta tattttttta tatcttcagt attatggaaa gttatacgta gtatattttg
    37141 tctaatgtaa ctacgtaaac ttgatggtgt tccttgtgct aaatcctttc cattcttcat
    37201 aatacaaata ttatcactca attgttcagc ttcttcaaga taatgagtag ttagaaatat
    37261 agttgtgcca tactcatctc taatttttaa taacatatcc cacatagatt ttcttgaatc
    37321 gacatccata ccaacagttg gttcatctaa aaataggatt ttaggagatg ataccatatt
    37381 catagctatg tccaatctac gttttacacc ccctgaatat gatgaagtag gatattttaa
    37441 gtaactagat aagtcaaact tatcaattaa agagtctatt ctttgttttg caatctgtgg
    37501 ttctacttta tacattttac tttgaaatac catattttcc ataagggata aatgttcatc
    37561 aatagagatt tgctgtgcta cacaagcaat ttgagtacgt atccaagaag gattatctac
    37621 taaatctttt ccaaacattg ttacatttcc agatgtaggt ttgtaaaagg tagtcaaaat
    37681 atttataaga gaagatttac cagcaccatt aggaccaagt aaagaaaaaa tttctccagc
    37741 atttacatta aagcttagag catttaaagc tttcacacca tttttatatt cttttactaa
    37801 attgtctatt tcaatcgcta acatatatta ttcctcctct ttaagtggaa atattatctc
    37861 tgtaatatag ttatctgggt ttccttttat caacatacct ggtcctttaa taaatacttc
    37921 tctccaagga gtttgtattt ttatattatt ttcatgaata tatttaaata tcatttcata
    37981 cgtttttgga aaattactat aagagcctgt gtgagtaaaa aataaagctt ttgttttagg
    38041 aaattctttt gtagttatac ccatacgatt tggagtttca gcagttggaa cacataattc
    38101 catttctgca acaccagttt tttggtcaac atcatagtag caaaagaatg gtgcaccgtt
    38161 agactttcct tttatagctt taaatacatt tggaaaatac tttgttgcct cagtcatagg
    38221 tccattataa cgcatggtag ccactgtgac tgattcgata tttttgattt caattttgca
    38281 attcattgtt tacctcctaa tgtgtgttca tattcttact agatttttaa gcattaatac
    38341 atataagatt ttgattagtt gaaaacatat aatcttatct cgtttgcttg ttttcaagtc
    38401 taacataaca taaaatatat atcctctcat ttgatgctcc attttgtaaa gttattattt
    38461 aagctttact ttagattttt tagcaaatta aaataaagca atttataaaa taagtattta
    38521 caaattaagt gtattaatcg tataatacac ttaataggat actagataaa gtctgaaata
    38581 aaggcgtatt tttttaaagt aagtgtacta tcgtattaat acactagaat aggaggaatc
    38641 atgaattttg aattagataa tacaactccc atttatttgc aaatagtaaa gtatataaag
    38701 agacaaatag taacaggaga gttgaaacca ggagagacaa taccatcaag aagagaaatg
    38761 gcacttaatc taaaagtaaa cttaaataca gttcaaagag catataagga gatgggtgat
    38821 atgaatataa taaatacttt taaaaattat caaagcagtg taacagttga tgaaaatata
    38881 ttaaaaaatt taaaattaga gcttataaat gaatcactat ctgtttttat agaagatatg
    38941 aaagcaataa atgttagcaa agaagaagtt ttaaaaataa tagaagataa gtattagttt
    39001 tttaacaaat agaaaactta gtttagaaag tttatttata agcatattgg atgtggagga
    39061 tagtaaatat gatagaaata aagaatgtca gcaaaacata taaaaggatg caagggctta
    39121 aaataagaaa gatagaagct ttaaaaaatg taagttttaa tatagaaaag ggaaaaataa
    39181 ctgcattact tggtataaat ggtgttggta aatcaactat gttaaaagca atagcaggac
    39241 ttataaacat tgatagtgga gaaattcgta tagatggaga aaaaataaat gaaaaggtat
    39301 acaataaatt ggcttttgtt ccagatgtac aatctcactt tagcaataca acaataaaag
    39361 aaacatttga atttatggaa attttttatt caaagtggaa taaagaaaaa tcaaaagaaa
    39421 tgatggacat atttaaattg gatgaagatg aaataattga taacttgtca aaaggaaaca
    39481 ttgcaagagt aaaattaata cttggatttt gccaagaccc agagtacata cttcttgatg
    39541 aaccatttac aggaattgat ttgtttaaga gggaagagtt tataggagta atagcgcaat
    39601 atatggagga aaatcaagcc attatcataa ctactcatga gatagtagag atagaaagtt
    39661 tagttgatga ggttgtaatt cttgatgaag ggcagattat aacttcattt aatgcagaag
    39721 aattaagaga aagagaaggt aaatctatat tagataagat gagggaggta tataagaatg
    39781 aataaacttc tatatctaat aaatgtagat ttgagaagaa gcaataagtt ttatatagct
    39841 tatatatcat ttttttcact aattacactt agtttaaatt tattcgaaat taataaattt
    39901 aaaaattcta aatttattat gaacttagca atagaagatt ttggaggtat attttatgga
    39961 ctagggatac ttagtaatct tggttttatc caaagtatat tgttctttgg gatattggga
    40021 ctttttatat actatatttt tatgtggaaa agagagttct tttcaaataa tcaaagtatt
    40081 tatacactta tgatgttacc tcaaaataag tttaagattt gtatatctaa atcaatagct
    40141 ttgataacaa tgatatatgg ttttttgata agtcagacag caacattatt tatatgtaaa
    40201 tatatattta atttcatttt tagaaatatg cctataatca atatgaactt tgccaaagat
    40261 ttatattatt taaacttgaa gagtatacca attgatttta tctcttttat ggctatctat
    40321 gtatttttac tacttatagc tatttctatt gtaaattgct gtatattatt tatcttttcc
    40381 tatataaata aattttatgt aattttaatc actttgctga ttatagcagg cttatatttt
    40441 tcaacaatat atataaatat aatcacttta tttagaaaat attatagtct tataggattt
    40501 caatttaaca atttagtagc agggttatta agttttttaa ttataatgtt tattctaaat
    40561 ggaatatctt atttgctaat taaaaagaaa gtatctcttt aaggaggggt ggtatgaaat
    40621 taaaatctaa aatatctata cttattatat ttttgacttt agcaatatat tttagtatag
    40681 gtttattaaa taatgaagat aatttaaaga taaaaactat aaaaggtgac tctaatgaat
    40741 tagaccatat aaacataaat tttggatatg ctttgaatgg atatataaat tcatattata
    40801 caataaatgg aagtaacaaa accaaaaaga ctgaattaat aaaactacaa aaggatagta
    40861 agaacacaaa taatttaatt gatgtatcta gtggttcaga tttaattgca catgttggat
    40921 ttgaaatgaa tgaggagtca aaagatataa actttgtaat agatgagaaa aaagaagatt
    40981 tagaaaaagg tgtatttcca tcagaaggaa gtgataaaga aattgaatat gaaaaattag
    41041 atatgaataa ttttacaagg acaagatata aagtagataa aagtacagat gattattatg
    41101 caaatatact agcatcaaaa agatataaaa aatacttata tgtatctgta ctttatgcta
    41161 caagtgattc atcagaaaat aacaaagaat attcaagtac tatagatata ataaaaatta
    41221 atccagaaaa taaaaaaatc aagactataa aaagtataaa tttaagtaat tatttttccg
    41281 aaaaatacca aaacatagag accataggtg taagttatgc tagcaaatat gaggataaga
    41341 tgtatatttt agtaaatgta ctaaatacaa acataggtga taatagtaat tctaaatttc
    41401 atttacttga gtataatctt gaagaaaata aggaaagcat aatggaatta aaaactgata
    41461 gcaaaatggc tttagcaaaa gcaaagttag aagctaataa attatatatg gtaatgtcga
    41521 atgattatga taatagttca attacagagt taaatttact tacctataat atattagata
    41581 aaagcttaga tgaagataaa tatgttttag ataatagaga aaaaagagga attagaagta
    41641 ttgaaattga ttcaaataaa atcgttgcat atacatcaag gctaaactct atattatctt
    41701 gggataataa atttatgcta tatataataa ataaaaattc taaggaaatt gtatatgaag
    41761 gagaattaca atttaacagt tcaaatagta tgttagaatt tgaaataaag taggtgagtt
    41821 gatgaaagat ttaataagat tattcgaatt tgaactaaaa agaaatataa agaattatat
    41881 atttataata atttgttgtt gttcttttgt aattttaaac atagtaaaaa atttaaatga
    41941 ctataattac ataatagaaa atgcagttaa aagtgaacag ttaacaaaaa tcggaaatac
    42001 gatagaaacg gtaaaggtta caggtttctc aagctttaga aatataatgg gttctacaga
    42061 atcgtggttt atgtttggta taattgcatg tatttgctat gcttttttta tttggtatag
    42121 agactttaat ggtagaagta aatctatata tacacttata atgctaccta aaaatagaat
    42181 caatatttat atatctaaat tattaaatat attatttcta gtgtatagct atacagtagt
    42241 acttacaata tctttgttta ttgcaagtaa gttgttgcct agacatatgc ttggtaatgt
    42301 cacaaactat ggttttgtac aagaaacaat atatgagtta aaaatgttac ttccatatag
    42361 ctttgaaata ctat
//
LOCUS       DAEOSP010000014        40056 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.014, whole genome shotgun sequence.
ACCESSION   DAEOSP010000014 DAEOSP010000000
VERSION     DAEOSP010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40056
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.014"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(112..1485)
                     /locus_tag="KRH54_000567"
     CDS             complement(112..1485)
                     /locus_tag="KRH54_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578240.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(1672..2994)
                     /locus_tag="KRH54_000568"
     CDS             complement(1672..2994)
                     /locus_tag="KRH54_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8578241.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            complement(2987..3691)
                     /locus_tag="KRH54_000569"
     CDS             complement(2987..3691)
                     /locus_tag="KRH54_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8578242.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            complement(3874..4938)
                     /locus_tag="KRH54_000570"
     CDS             complement(3874..4938)
                     /locus_tag="KRH54_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG8578243.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFVMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            complement(4941..5639)
                     /locus_tag="KRH54_000571"
     CDS             complement(4941..5639)
                     /locus_tag="KRH54_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG8578244.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKKVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            complement(5630..6727)
                     /locus_tag="KRH54_000572"
     CDS             complement(5630..6727)
                     /locus_tag="KRH54_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBG8578245.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            complement(7050..8294)
                     /locus_tag="KRH54_000573"
     CDS             complement(7050..8294)
                     /locus_tag="KRH54_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG8578246.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            8622..9410
                     /locus_tag="KRH54_000574"
     CDS             8622..9410
                     /locus_tag="KRH54_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG8578247.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            9410..9883
                     /locus_tag="KRH54_000575"
     CDS             9410..9883
                     /locus_tag="KRH54_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG8578248.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(9975..10415)
                     /locus_tag="KRH54_000576"
     CDS             complement(9975..10415)
                     /locus_tag="KRH54_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBG8578249.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(10430..10723)
                     /locus_tag="KRH54_000577"
     CDS             complement(10430..10723)
                     /locus_tag="KRH54_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBG8578250.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            complement(10766..11167)
                     /locus_tag="KRH54_000578"
     CDS             complement(10766..11167)
                     /locus_tag="KRH54_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578251.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            11400..13520
                     /locus_tag="KRH54_000579"
     CDS             11400..13520
                     /locus_tag="KRH54_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8578252.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            13745..14911
                     /locus_tag="KRH54_000580"
     CDS             13745..14911
                     /locus_tag="KRH54_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG8578253.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLYRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIINPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            14936..16378
                     /gene="nhaC"
                     /locus_tag="KRH54_000581"
     CDS             14936..16378
                     /gene="nhaC"
                     /locus_tag="KRH54_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG8578254.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            16381..16650
                     /locus_tag="KRH54_000582"
     CDS             16381..16650
                     /locus_tag="KRH54_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578255.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(16727..18154)
                     /locus_tag="KRH54_000583"
     CDS             complement(16727..18154)
                     /locus_tag="KRH54_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBG8578256.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKMGQLSYGSKVETVEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            18526..19395
                     /locus_tag="KRH54_000584"
     CDS             18526..19395
                     /locus_tag="KRH54_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG8578257.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            complement(19507..20313)
                     /locus_tag="KRH54_000585"
     CDS             complement(19507..20313)
                     /locus_tag="KRH54_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBG8578258.1"
                     /translation="MGRKVKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(20327..21559)
                     /locus_tag="KRH54_000586"
     CDS             complement(20327..21559)
                     /locus_tag="KRH54_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG8578259.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMNCFGIKDK"
     gene            complement(22018..23805)
                     /gene="aspS"
                     /locus_tag="KRH54_000587"
     CDS             complement(22018..23805)
                     /gene="aspS"
                     /locus_tag="KRH54_000587"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBG8578260.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            complement(23879..25141)
                     /locus_tag="KRH54_000588"
     CDS             complement(23879..25141)
                     /locus_tag="KRH54_000588"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBG8578261.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEKANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            complement(25131..26687)
                     /locus_tag="KRH54_000589"
     CDS             complement(25131..26687)
                     /locus_tag="KRH54_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBG8578262.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKETKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            complement(26778..27392)
                     /locus_tag="KRH54_000590"
     CDS             complement(26778..27392)
                     /locus_tag="KRH54_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8578263.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            complement(27407..27856)
                     /locus_tag="KRH54_000591"
     CDS             complement(27407..27856)
                     /locus_tag="KRH54_000591"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBG8578264.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            complement(27881..30088)
                     /locus_tag="KRH54_000592"
     CDS             complement(27881..30088)
                     /locus_tag="KRH54_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBG8578265.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            complement(30162..30674)
                     /locus_tag="KRH54_000593"
     CDS             complement(30162..30674)
                     /locus_tag="KRH54_000593"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBG8578266.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            complement(30687..33137)
                     /gene="recJ"
                     /locus_tag="KRH54_000594"
     CDS             complement(30687..33137)
                     /gene="recJ"
                     /locus_tag="KRH54_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBG8578267.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            complement(33150..34814)
                     /locus_tag="KRH54_000595"
     CDS             complement(33150..34814)
                     /locus_tag="KRH54_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBG8578268.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVIAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            complement(35112..35609)
                     /locus_tag="KRH54_000596"
     CDS             complement(35112..35609)
                     /locus_tag="KRH54_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBG8578269.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            complement(35638..37017)
                     /gene="scfB"
                     /locus_tag="KRH54_000597"
     CDS             complement(35638..37017)
                     /gene="scfB"
                     /locus_tag="KRH54_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBG8578270.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            complement(37322..37468)
                     /locus_tag="KRH54_000598"
     CDS             complement(37322..37468)
                     /locus_tag="KRH54_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG8578271.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            complement(37498..38076)
                     /locus_tag="KRH54_000599"
     CDS             complement(37498..38076)
                     /locus_tag="KRH54_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG8578272.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            complement(38444..39874)
                     /locus_tag="KRH54_000600"
     CDS             complement(38444..39874)
                     /locus_tag="KRH54_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG8578273.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEICLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
BASE COUNT        13156 a         6891 c         4506 g        15503 t
ORIGIN      
        1 taaaaatatc aataaaaata gatgtatggc ttagaattag taaaacaatt ataataaatt
       61 atagctacac atctattttt aatttataca attaaagatt tattcgattt attaaataat
      121 aaacattgct actatggttg ttacaaccaa tccggttata actgggacaa agtttcgctt
      181 tgccaattct attgggctaa ctccacaaat tgccgctgct gaaataagac cccaaggaac
      241 tagacagcct ccacctaccc atataccact gatttgtcct aatgcagcta gtgcaccaac
      301 acttgcattt atagcagttc caaatacaga ggcaagtgaa ccaactaatg aaatcccaga
      361 aaatccagaa ccatcaagcc cagttattgc tcccactaca gtttctatac ctgatacggc
      421 aaatttatta agtggtattg tttgagaaag agctatgcct atatctgata ataaaccttg
      481 tgaacctggt gctaatactt ttccaaatac acctgtcaat ggtgcaactt cacccatata
      541 gaaaaatgct gctattggta taattgctcc aaatattttt atcccaaaca caaaaccatc
      601 aattatattc tcacatacat tttcaagact atctttgcca aaatttataa catttataat
      661 tattattaaa aatactgctg ttccacccaa aagagctgat gcatctcctc cttttaggtc
      721 aaatatatac atagcaatta tatcagcaag aaatcccaga gctactaaaa tagttgctat
      781 tttagatttt ccattaaact cttctacttc ttcactttca aaacctttta gttcttctct
      841 aaacattcct ttgttaatat ctctttttaa tgtataaaat gccatagata tggttacgat
      901 tcccatcacc caaaacaaaa tcataccatc atttataaca tatgatactg caacacctgc
      961 tgcaccagct gttatacttg gagctccttg aataacaaag tctgtagata aggcaagacc
     1021 atgaccaaat aaatttaatg ctactgcaac accaattgct ggtagcccag ctctcatagc
     1081 aacaggtaaa aatacagctc caactaaggc aacagctgga gttggccaga aaaaccaaga
     1141 aagaactaac ataacaaatc cagttacaaa aaatgctgta ttagaatttt ttataactct
     1201 cgaaaatggt ctcaccatat actgtatagc attattggct tctagggctt tagataatgc
     1261 aaccataata gctataatca agataattgg acccaactca tttaaagata taataaaaga
     1321 gttaaacact gctgttatag caccaaaaat gtttttttca aagaacaacc ccataaaaaa
     1381 aacacctaat atacatggta ctattgtatc ctttttcata atcatagtaa ttaatataat
     1441 taccataaat agaatgtaaa tataatgtgc tcctgtcaaa accatattat tatgtcccct
     1501 ttttttaaga taataatatt atatggtctt ataagtaaac ttgttacttg cctagaggta
     1561 cacaatattg aacatcccat aactaaagtc acatgcttcc tgcttcatag aattttacat
     1621 aagcaattac acaatatata atagcaatta cacaatatat aatttaaatt tttatgattc
     1681 ttttgctgga atagatattt caatagcaaa ttctttatca tctgaattta catttatgct
     1741 tccattatga tatgcaacta ttttctcaca attctttata ccaattccaa aactttctac
     1801 attcttagaa aaattactta ctctattttt ttgtataatt agtaatttag aatcctctac
     1861 tttaattaaa aatactatag gcttatcttt cctagcatat tttattatat tagaacataa
     1921 attatcaaat atccttctaa attgtgtaac atctacataa atatgatatt ggttataaag
     1981 ttcattttct attgttatat gaaatccttt ctcctccaat gtatatgaac tatctaaaag
     2041 taattgaaaa ataaattcat tagcatttat tttttcatac ccttttgaaa cttcctctcc
     2101 agaattaaca aaatgcgtaa aaaggtcatc tgttaaatct tttatttgat atgctttaga
     2161 ccttacattt tttatgtatt gttccaattg ctcaggacta ttgtatttat ggtctttaat
     2221 caaatctaaa taacaaatta ctgacgttaa tggagttcta acatcatgtg atatcgaagt
     2281 tactatatct ttatttttat tcttcatctc cctttctttt ataagttgat tctccaatgt
     2341 atgactcatc tcattaatac tagatgcaag tgatgataat tcatcattac cttttactgg
     2401 gattctatac tttaaactgc cactctctac tatctttata cccatttcta ttttccttag
     2461 gtactttatc tttctactaa acaacagtaa aaatgttata ataaatgtaa gtgccataaa
     2521 tgtaatagaa attgcttcta taaaattttg aaatctaaat ctaaataaag ctactacaac
     2581 tatattagct ttaatatcag aaaattgtat ctcatagttt ctagccatgg gactgctata
     2641 aaactcattt tcctcactct tatcaggtac ttcattagga tagtataatg tatcataaaa
     2701 aaccttattt cctttatata tcttcataga aacaaaccaa ttatcactat tccattttgc
     2761 tatatcatct ccatcatctg ctcttatacc attgttattc acataagttt gaaaattatc
     2821 cataagttga tttacttctc ttttaaaata tactttccct tctgatacat cttttactat
     2881 agattcaaca gcagagtaaa atggtatatt taaaatgaaa gccactgcag ctgagattac
     2941 tacatatatc aatagtataa ctcccaactt agatggctta attttgttaa tcaatataat
     3001 acccctttcc ccatgcggtt tttatatatt gtggattttt ttggtctttt tctaactttt
     3061 ttcttaaatt tctaatatga accattattg tattatttgc tgtataaaaa tactgctcat
     3121 cccaaatagt ttcatataaa ttttcagacg aaaaaacttt ttttctattt ttcaacataa
     3181 gaattaatat actatattct atagttgtca aaaacacagg ttctccattt agtgtaacat
     3241 cttgagtatc taaattgaca cataactctt ttgtttttaa tatatttggc tctctttcct
     3301 tttcctttcc ctggtatata taataacgtc taagtagaga cttaaccctt gaaattagtt
     3361 cagaatatga aaaaggcttt gataaatagt catctcctcc tgctgaaaat ccaataactt
     3421 tatcagagtc ttgagttttt gcagttaaaa ataatattgg tgctgtcgtt ttttctctaa
     3481 tttcattaca tgcaacaaat cctgttttta caggcattac aacatccaaa ataatcaaat
     3541 caatcgtact atctactaag tttacagctt cttctccatt cgttgccata ataacttcat
     3601 aaccttcacc agttaaaagt atttctacga tttccctaat ctcaggatta tcatcagcta
     3661 ctaaaatttt ttttggaatc tctttattca tcaccaagct cctttttttg ctatatttct
     3721 taaatatttc aaactatata aaatattata ctttattagc aaaagcttag caatgtcaca
     3781 cgttttaata gtttctactc ctgtaaacat gtttaaagct taattaaaat atacacacaa
     3841 attgacatat taataaaaat aatctatata atattatctt acataagcaa cttttttcac
     3901 tgcttttact ttttcagaag attttacatg tactactata catatcaatc cacttatagc
     3961 tacaaatgca taaaaactta tccccctact aagtaacatt gccggaacta ataagtgacc
     4021 agggaaaaat atcttaaata atgtcataaa actattttca gaagctccaa cagcccctgg
     4081 caatggtaat gaagaaacag caatattaag caaggattgt atcgctaata tctcaaatac
     4141 agtatatgtt gatagatgaa aagctttgta tacaaagaaa ggaactaaaa acatagctgt
     4201 tatttgtaca atagttatga ctaaaatttg gactacaagt tttggattct gctttatata
     4261 ttgagcacct ttaacatact cttcaatttg agatattata acttttcttg aactttcaac
     4321 atcttttatt attcttaact tacctaataa atttgttatt gatattataa aattgtttac
     4381 aagttttttt gaaaatataa ttgcaattac tccaatgaca agagcaacat ttgttattac
     4441 accatatatt agtaaaatgt tcataccact tacattattt aatataaact cacgttccat
     4501 aatgaacatt attccactat atgccagtat aactacttgg tgaataacaa caatgactaa
     4561 tagtataagt gaagaatatg atatatttat atcgtctttt ttcatataat aaacttgtgc
     4621 tggttgacca cctgatgctg aaggtgtaat agaactaaaa taaaatccaa taaaagcata
     4681 tcccatacat tttaaaagag tagtcttttg gtttaaagct ttcataccta agtatatatt
     4741 tataccttca caggcaacat atataaacat cattacaaat ccaattagta taaacatagg
     4801 gcttgcacct tttaatgatt ttatcaatga ttcaactgac tgccctctaa aaacaaaata
     4861 tcctgttatg cccattagta aaactaaaaa agctacacta cctaggcatt tatttttctt
     4921 ttcagtttta cttatcataa ctacatcctt tctggtgtta cgaactttaa accactggct
     4981 ttaagttttg gaatagctat ctttaaagct tcaatagtac gctcaggtgc acctactgca
     5041 ccacctgaat tttcacctgc atcatgtaaa caaattatag aattttcttt tgttctactc
     5101 attaacttgt ttataataat gtctactgta ctatctttct cccaatcttc tgccattaca
     5161 tcccacaaag tcatttttag attatatttt ttaacaaatg aatttgaaaa tatattagta
     5221 tgaccccatg gtggacgata gtagtttaca tctacaccta aatttttcat tatatttgta
     5281 ctttcaataa aatctttttt aacataacta taactataaa gccatgcatt tctgtgttcc
     5341 aatgaatgta aaccaactat atgattttct ctcaacattc tttttataat ttcaggctct
     5401 ttttcagcat tctttgctac tacaaaaaac atagcttgta tatcattttc ttttaacaca
     5461 tctagtaatt tttctgtata tctcttatct ggaccatcat caaatgtaag tgcaatttca
     5521 ttttctccag ccatattttt taataccttt ttatttaata gtttattata gtatgtaggt
     5581 ataattgaat ggactaaaaa aatcaatgca acaacgataa ttaaacctac tacatacatt
     5641 ctctacacgc tccttgttca tcaagtgaaa ataaaatatt ctctaaggta ttattatcaa
     5701 attgtttttt taattctttc atgttactgc tcatttcttt aagagtagca tcatcataaa
     5761 ttaaatcttt tatctcatct acatagtatt ttttattttt accaagtatt ctcccaattt
     5821 cattatttaa tataaaacta gcattatcta tctcttgttg taaaaatgga ttaaatgcca
     5881 atattggaag ttctgaataa attgtttcaa atagtgttat tccacctggt ttagaaataa
     5941 taaggtcaga atctttcata tatttatata cttcattggt gtaacctact acctctatat
     6001 tttcatatct accatatagt ttatagtaca tttttttatt attaccagtt attacagtag
     6061 tttttactcc ttctaaacta tttaattctt tgtaaaattg ttctgattta ggcagtaatc
     6121 ctaatcctcc acccattata agaatatttt ttttagttga atgatttaca tgttctattc
     6181 ttttaaattc tttttttact ggaataccat taactttaat cttagattca tctattcctt
     6241 taaaaacaag ttcttcctta gtagatttac tagctactaa ataacagtct gtatttttac
     6301 taatccattc tgagtgacta cttatatcag ttatacatgt tattaaagga agatttgagt
     6361 tatatttttc cttatatctt gatactaatt gagaacaaaa tggaaaagtt gaaataacta
     6421 ctgttggttg tacttcatga agtaatgtat ctaatttatt taaaaaataa tctgaaaaag
     6481 taaattttat atctttcttt ccattttcag cacatttata aaataaatta taaagactac
     6541 ttccccgatt tacaagaata gaaaaagtct tgtacatttt atctgaatag ttaggcataa
     6601 tgtactcaaa tatatcttta attattattt ctgaattatc aaattttgct tttatatctt
     6661 cacttaaaga atttgatgct gagtaatgac ccataccaaa ttttcctgta agaattaata
     6721 ccttcataag attccctcct cgttaaagct tttcttttca tatgattact atacttataa
     6781 gtatagactt taaatcttta gaaaaaccta gtataattct taagaaatct taaagtttta
     6841 attaaaaaat acaaagagac catctaaaaa tagagataac catatttttt attatttaaa
     6901 taatacaaat atgttcgatt ccatacaaaa aagtgcctca taaactaaaa agttcatttt
     6961 gagacacctt ttttatatct aaatatattc aaatacatac acaatcattg taattattta
     7021 aatgtatttt atttagttgt tctttataat taatccataa tattatattc ttcatataaa
     7081 ggatattttt cagttaaacc aacaaccaat gttctagcct tagattcttc atcataagtt
     7141 aaacataggt ctatagcctc agcgattatt gccatatctt cttctttcat acctctagta
     7201 gttacagctg gagtaccaag tcttattcca cttgtaacaa gagcaccatt tgggtcaaat
     7261 ggaattgtat ttttattagc tgttatataa gcatcatcca atctcttttc tgctgcttta
     7321 ccagttatat ttttattagt caaatctaat agtattaaat gattatctgt acctccagaa
     7381 atcaatttaa aatctctttt aataagttct tcagctaagg cttttgcatt tttagcaact
     7441 tgtacctgat attctttaaa ctcatcactt aaagcttctt taaagcaaac tgccttagaa
     7501 gcaattatat gttcaagagg tccaccttga ataccaggga atattgcttt gtctatgtct
     7561 ttagcatatt tttctttaca aagaataaca cccccacgag gtcctcttaa agttttgtga
     7621 gttgttgttg taacaaaatc agccacttca catggatttt ggtgaagacc agcagccaca
     7681 agacctgcta tatgagccat atctaccata agcaatgctc caacctcatc agctatctct
     7741 ctaaactttt taaaatctat ttctctagga tatgcacttg ctcctgcaac tatcatttta
     7801 ggttttactt catgtgctat ctttctaact tcatcaaaat ctataagtcc actgtcttta
     7861 tctataccat attcataaaa tttataatat tgaccagaaa tattaactgg agcaccatga
     7921 gttaaatgtc caccttgaga taaattcatt cccataacaa catcacctgg ttttaacata
     7981 gcaaaatata caccaatatt tgcatttgct ccagagtgag gttgaacatt agcatgttct
     8041 gctccaaata tttttttgat tctatctatt gcaagagttt caacttcatc tatgtattca
     8101 catccaccat aatatctttt gcctgagtaa ccttctgcat acttatttgt aagatgactt
     8161 cccatagttt ccattactgg aactgatact atgttttcag atgctattaa ttcaatattt
     8221 ctttgttgtc tttttaactc tcttttcata ctttcataaa tctcagggtc cgcttttttc
     8281 aagttctcaa acatattact cctcctagtt attatttaaa ttttattttt acagacattt
     8341 cataattttc gtttttatca atatgataat ttattattat agtcctttac caattaatag
     8401 tcaagtgttt tgccattttt ccatatttaa catagttact ttctttattt ttaagttaaa
     8461 atataataaa taatattgtc taattctttt aattggatat aatttaaatg tgactatttt
     8521 gaaacaaaaa aaagataata tatttatata gatattaagg aatattactt aaaaatttga
     8581 tataatttca atattacgtt ttttaatttg gaggtgtatt tatgaatcaa aatgaggaac
     8641 ttgaatataa gaaaaatatt ctaaaactag ctctatttgt aggggaactg atgataaaaa
     8701 atggagctga aacatctagg gttgaagaca gtgtccttag aatatgcagg tcaagagggt
     8761 tttatcatgt aaatattttt acaactccta cagtaataat tatttcagat gagaaatttg
     8821 atggcttttc gtttatgaaa acgattcaat caagaggtat aaacttaaat aagatttctt
     8881 tattaaacag tttttctcga gaatttgtta gtgacaaaga gtatgatatt gataatgcca
     8941 ttgcacgtct ttatgaaatc caagatgtaa aaccttatcc tttatggtta ttttactctt
     9001 gtacaggaat tgcatctgct tgttttgctt gtttgcttgg agggaattat gtattaaatt
     9061 ttatacttac gtttataata gcaatctttg ctgcaattac ttatgataaa acgatgaaga
     9121 taagtgcaat atctgcattt tcctgtttag tatcttcttt tttaattgca ctttcaggag
     9181 ttctactagt tgaaataggc attttagatg accctaaaat gcttatagtc ggcgctataa
     9241 tgccactact cccaggagtt gcttttataa aagctattag agatttaata tcagggaatt
     9301 taatatcagg aatttctcgt gcttttgatg ctgctatgat tattattgca attgcttctg
     9361 gagttggttt tgtccttgat acatggtata gaataggagg tcctttctaa tgactgatat
     9421 gtctatgatt ttgcattttt tattttcagg tgttgcaact gctggatttg ctgttttctt
     9481 taatgctcca ctatatctac ttcttccagc tggagtaact ggcggaattg gttggattgt
     9541 atatgtttat ttatttaatt ttactacaaa tgcagttttt gcaggtttta tcgctgctgc
     9601 tttggtctca gcgtgcagtg agactttagc aagaaaacta aagcaaccag ctattgtgtt
     9661 cgtaattcca ggtattcttc ctctaatacc aggaattggg ctatataata caatgttgtc
     9721 tttgattcag aaaaattatt ctttggctat gtcaaaaggt acagatgctc tatttctaag
     9781 tgctgcaata gcacttggtg tattagttgt aacttctttt gtaagaacat tgaatctttt
     9841 aaaaatcaga aaaaacttta ttccttacag aaaatcaaaa taaaaatata aaaatagcaa
     9901 tgtacttaaa ctctacaaat acgtattgag tatttattta aaaagtacat tgctattttt
     9961 taattataat tatcttaaag gtcaattaat attcttgtta ttactggtat aaattctcta
    10021 ctgtctctaa attttttgtc attcttcttt aagtataaat aacagattat tgtaagacaa
    10081 attccaacta atccacttat ggcttcaact ccagttgtca ttccaattgc ccccaatata
    10141 taataagtcc ctattgttcc tacaagaagt gctataagag gtattatata agccattaca
    10201 gctgctttta atacattcat attatccata ctcacttcta catggtcacc aacttttgca
    10261 ccaattgtgt tgtctacttc tactaatatt tcttttgact ctgctgaatt tgtagtagca
    10321 cattttccac aagcagcaca agctgaatgt ctttgcattt ttaactttgc agttctatca
    10381 tcaacaattt ccataataaa tccttgttga ttcataacta accgcctcct tattttgtaa
    10441 atttatttat agtatccata agctctttta tcatctcttc agttttatca gcatcatcat
    10501 gtttaatgct ttctgctaca catcctttta tatgattctc taagataatt gtgcctactc
    10561 tatttatagc agaccttata gcagatattt gaaccaatat atcaacacaa taacgttctt
    10621 gttccaccat cttttgtata cccttaactt gaccttctat tctttttaat ctttttatga
    10681 gagcttccct ctcattgttt ggtgctaatt ttctttcttc cataaactca cctcatactc
    10741 taatgatgtc ttatacaatt atgctctact taccaacttg tttaaaatat aatacagtta
    10801 ttattactat aaatgccaaa attgatattg caatagacct actttgattc ttagtttgtt
    10861 ccatacttaa ctttccattc atcttaaatc ctgccaatgg gaataaaaat ataaccagtg
    10921 catcaattcc aagtacaact tgggctgcaa tagtccattt actcaaaaag cagaatattc
    10981 cgcccacacc taaaagaacc tctaatatat ttataaactt atatgtaggt ttttttgcta
    11041 tatattcttc aattttttcc ttacattctg cacgacatgg atatatattc atattgtcat
    11101 catattttat agctaattta ttatttggtg actttatatc cctattacag taaagacatt
    11161 ttttcatttt atattctcct ctagtcttcc ttaaaaaatt ttgcctaaat tattatacgc
    11221 tctaatacta ttataccaaa tagatacaaa aaacaaaaat aaaaggaata attttagcta
    11281 cttcttttac ttcaatgtgt agtctataat aaatttataa taataattaa ataatattat
    11341 ttaatctaat ttaattatta tgtaatctaa aataagtcaa atttgtatga gaggttaaaa
    11401 tgaaacaaat ggaattagct attgtaagtt taaaaaaaga cgctggagaa atttatgaaa
    11461 atcaaataag acaattctta ggtgataatc taaagataaa tttatactca tttgaagagg
    11521 gaaatttaaa gttttttaaa gaaaaactta tactcctatc tgcttattta aaatatgatg
    11581 aaatagtaaa attatctcac tatgatgctc aaatcattgt tcccaagttg acatttgaaa
    11641 agaacagtat tgatatgata tccaaattag aaaaagataa aattatttat gtatataact
    11701 taagtaaaga tatggcaata gaaacaattt ctttaataca tagattagga atagacaata
    11761 taaatatcct gccctgctat ccagagattg agtttactcc cacagatgct gtaatcctaa
    11821 ctcctggcga aaaaatcttg cctaagttta aaaattgtga agttatagat ttaaaataca
    11881 gaataataga tttaagttgt attgttgaaa ttgctacaaa aaccaaatta aaacatctaa
    11941 taaaagatga cctaataaaa aaatacgtag aaaaaataat tcctacaagt tatagtacag
    12001 gtgagttatt gttagatgca aataagtttg aaagacaatt tgatttgtta ctatctataa
    12061 ttgatgatgg aattatatgc acaaataacg atggaataat tcaattctat aatcatatgg
    12121 ctagaaaaat tttgtctata aatgcaaatg aaatgattga ctcatttgtt ggtgattgca
    12181 taaaagacat caattttcaa agcatattga ccaataaaac tccatttttt gagaaattga
    12241 ttaaaataaa tcatattgat ataaatttag agattaaaca tatacaatta aatgtatttg
    12301 atggatttat actaaagatg acaaagtttt ctcaattaga aaaaaagcaa gctaaattaa
    12361 gagcacaact agtaaactct ggtaatatca gtaaatacac atttgatgat atattaggtt
    12421 ctagtataca aactattaat acaaaaaaaa ttgctaataa aatggctcaa tcaaattcat
    12481 ctatacttat aattggtgaa agtggtactg gtaaagaatt atttgctcaa tccattcata
    12541 gtgcttctag gagaaaagat ggtccttttg tcgcagtaaa ttgtagtact tttcaagaaa
    12601 atctactgca aagtgaatta tttggctatg atgaaggggc ttttactggt gctaaaaaag
    12661 gtgggaaaat tggtttattt gaattagcca acaatggaac tatattttta gacgaaattg
    12721 gagaaatgga tttaaattct caatctaaat tacttagagt catacaagaa aaacaagtac
    12781 gacgtattgg ttctaataat gtcattgaca ttgatgtaag aataattgca gctactaata
    12841 gaaatttaaa agaacttgta tctaaaaata tgtttagaag agatttatat tttaggttga
    12901 atgttcttcc ccttaaaata catccactta gggaaagacc tgctgatata tttgaaattt
    12961 ttggttcatt aaaatatgat attccatgta attttattct aagtgaggaa gtaaaagaaa
    13021 tctttaaaat gtatagatgg gaaggaaatg taagagaatt aagaaactta ggagaatact
    13081 tctgttactt agaaaaagat attatagaaa tatgtgattt acctgaatat atattggata
    13141 ctatagattc aaattatagt agaaccgttt gtaataaagt aagtgataat ataaaaaagt
    13201 atcaattcaa cattggcaaa gacaaaaaca tcatgaaata cgactataat tttaaaaggt
    13261 cactagatga atacatattt atattggata atttaaaaaa ggcttatgat ttaaaagaaa
    13321 gaataggtag aaaaagctta tgtaaaatag ctttagagga aaatagattt ttgacagaac
    13381 aacagattag aaatatgctt ttagaactac aagactttgg gcttgtagac atacttgttg
    13441 gtcgtggagg ttcaattata acatcaaaag gagtagaatt tctaaaaaat ataaataggt
    13501 ctaacaagtt aaacagttaa acctatttaa cctatttaat tacattttca tatatttaaa
    13561 aatttattag aaataacctt ttccacaaag ttttaagtat aattaatatt tttatcttat
    13621 ctatgctctg aaattactta atattaaata ttaacttgtg aataaatatt ttttattatc
    13681 aatattggca cgttgtttgc ttaatattat taacataaaa ttaaaattac tggaggtatt
    13741 tacaatgaat tataatttta atgagattgt tgatagaagc aataattttt catctaaatg
    13801 gtcagaaatg gaaaaaaagt atggaactaa tgatttactt cctatgtggg tggctgacat
    13861 ggattttaag gctgcacctt gtataattga ttccttaaaa aatagactag aacaagagat
    13921 ttatggctac acaacaagac cagattctta caatgaatct atagtaaact ggctatatag
    13981 gagacataac tggaaaataa aaagtgagtg gttaatttat agtccaggtg ttatcccagc
    14041 tataagtctt ttaataaatg aactcacaaa agcaaatgat aaaataatga tacaagaacc
    14101 agtttacagc ccttttaata gtgttgtaaa aaataacaat agagaactaa ttataaaccc
    14161 attacaaaaa ctagaaaatg gaaactacat aatggattat gaagacattg aaaacaagat
    14221 taaagatgta aaattattca tactttgtaa tcctcataat ccagttggta gagtatggac
    14281 aaaagatgaa ttaaaaaaac taggagatat ttgtcttaag cataatgtaa aaatcatatc
    14341 tgatgaaata catagtgaca ttatattaaa aaaacataag catattccta tggcatctat
    14401 ttctaaagaa tttgaaaaaa atacaattac ttgtatggct ccaactaaaa cttttaatat
    14461 agctggtctt caatcatcat atgtaatact acctgatgaa aaagactata aattgttaga
    14521 tgatgccttt accagaattg atataaagag aaataattgc tttagcttag ttgcaactga
    14581 agcttcatat aataatggtg aatcttggct agaatcattt ttagaatatt tagaatctaa
    14641 tatagatttt gctataaaat atataaatga aaatatgcca aaacttaaag ttagaaaacc
    14701 agaaggtact tatttattat gggtagactt tagtgcactt ggattaagtg atgaggaact
    14761 agaaagtata cttgtacaaa aaggtaaagt tgctctaaat caaggaaaca gctttggcat
    14821 tggaggaagt gggtatcaaa gaataaactt agcttgtcca agaagtatgc ttgaagaagc
    14881 tcttataaga ataaaaaatg caattaacta aaaatattta aggagaattt gcactatgaa
    14941 aatgaataca gaaagaaaaa taccttcatt tagatattcc ttgttagtaa tgcttgcaat
    15001 aatagcccta acatcagttg gtatagttgt atttaatgct tctataacta caatgttttt
    15061 actttcatgg cttatagtag ttcctgcagc catgaaactt ggttatacta atgatgaaat
    15121 agaagctttt ggatttgaag ttggtaaaga tgcctttcaa tcaaacttaa ttatactttc
    15181 tgttggtgta ctaatagcag cttggatagc tgctggtaca ataccaacag tagtatatag
    15241 tggactaaca ataataaccc ctaaatattt tctgcttaca acattaatcg tatgttcatt
    15301 aacttctgtt gctactggta catcttgggg gactcttgga acttctggta tagcaatgat
    15361 gagtattggt actagtatgg gcattcctct tggtttaaca gctggtgcag tcatttctgg
    15421 tgctttcttt ggagataaga tgtcacctct atcagattct acaaacttag ctgcagcagt
    15481 ttgtaagaca gatgtaatta cacatatgaa gcatatgttt tataccacag gacctgcata
    15541 cgtaatatgt atagttctgt atacagtaat tggatttaag tactcaaata atactataga
    15601 ctatgttcaa ataaatcaaa tcaaggatgt attaaattca aactttcata ttggtttagt
    15661 agcaatgatt ccaataatat ttttattatt acttttattg ctacaaaagc cacccattat
    15721 atctatacta agttcagcta tcatgggact tattatagca gtatttcaag aaggagaaaa
    15781 aataggtgat gttttaaatt acatgctatc aggatttact atagatactg gatttgtcta
    15841 tgccgacaaa ctattaaatc gtggtggaat aatgagtatg gctgaaaccg ttttactagt
    15901 atttgtagtt ttcgttattg ctggtattct ccaaaaaact ggattcttag aagtactgct
    15961 tcaacctctt ataactaaaa taggaaaatc aagaactaaa ttggttgggt ctacttttat
    16021 agtaagctac tttgcaaatg catttagttc atcaatgatg ttcacttctg tatttgtcgg
    16081 aactctaatg tctccaatat acaaagaatt taaattaaaa ccacaaaatc tatctagaat
    16141 tatagaagat acagctacac ttggtggtcc attaatacca tggaattcaa atgctgtttt
    16201 ctgtgctcag actcttggtg taagcccact agaatttatt ccatattgtt tcttaagttg
    16261 gattacacct ataatttcat ttatttatgg tgtaactggt tttactatgc ttacatacac
    16321 agatgatgaa attgaagagt tagaaatatt tgatactgct cagagaagag ttgattgaat
    16381 atgaaatttg aatactatta cttaattcag gatattgcag gtatattatt ggcatttata
    16441 ggacttagaa tgtctattat tggatttagg atactttcta tgaaggggct ttctataaat
    16501 acacttttaa ttgtaattaa atactgctta tttaccatag caggactgaa tttattaata
    16561 tctaagtttg gaattagaca ctggatttgg agtgtttgta tgttgataat atccataata
    16621 ataaatccca ggattaaggt atcaaaatag aaatagttat tagacttaaa tttttataaa
    16681 aaaactgctt tacaaattaa agcagttttt ttattgttac ttataattaa ataactggtg
    16741 tatcagataa gtaatcatta cttacataac catatccacc attatacttt atttttaccc
    16801 atccagtagc aaacatttct actgtttcta ctttgcttcc atatgaaagt tgacccattt
    16861 tttcatcttc tgtacttggt cctttcctaa catttagacc atctttttct tttacatact
    16921 ttatttttat attttcatta ctattatcag gcttattatt tgtcaaataa gacccactaa
    16981 catatccaat accttcattg tattttattt ttacccatcc agtttttaac acatctaaaa
    17041 cttctacttc atcaccatta tttagatatc ctataacaga agcttctatt gtagcatcat
    17101 ttcttatatt taccttatct gtattagtta catacattac tctcttattg ttactatttg
    17161 cctgtaaatc ttttaattct ttaaatgcat tttcatttcc tccactacca acttgagtta
    17221 tagaggtagc atttttattc gaaaatacac tcttttgtgt tgcacttaat tgttctccta
    17281 cgattagtac aggagaattt tcttttgatg ctagtactcc tactgctaga gcgtctataa
    17341 gttgattttg atttttcatt ccatccttag ccacaaatac attgtttaat ttattagatg
    17401 tataaaactt ttctattact ttaccattag tttcatttct atctgctcca cctattcttt
    17461 ctgaattagg taaactttgt tttattttat ctgatacagc attagtagag cctattatgt
    17521 atgatttatt tatattctta gattctataa aatctttaaa gctagatata ccagatgcat
    17581 cagatactgg aagtataacc attccattag tagcagctac agaagctata ctaactgcat
    17641 ctgcaagacc tgtgtaccca tttactacag ctatttctga tatatctttt aatccttcta
    17701 tttttttagc tatactcaaa gctgtttggt atctatcact accaccaact ctatcagtag
    17761 ttaaattatt agattctaac tgtttttgca catcatttga tacagaacta gatagaccaa
    17821 ttatataaac ttttttaact cctaatcttt ttaattcttt ctcagtttgt gaatttagat
    17881 tatttttttc tgtcaataat ataggagcat tttttaattt agcaaatggt gttacagcta
    17941 atgcatctac aatagattca ctatttacaa ctacagcctc atttgcacta ctccatcctt
    18001 ttttacttac ttcaacagca gtttgatatc tatcctgacc tactaaagta ttctcaactg
    18061 ttgttctact tatttcattt gcatatgcta ctggtgataa gcttaaaact agagataagc
    18121 acagcattga tgatattgat ttagttaatt tcatcttgac tccccctaaa attatagtat
    18181 ctaaatgttt cctatttaaa atagtataac ataataattt aagtaacatc attattataa
    18241 aatattattt ttcttatcta ataagctttt attcttactt gacattaata ctttgctcat
    18301 caaatgaatt ttttatataa ctaaagtttg taacaatatt tccttagtta atcatttaaa
    18361 gttacttttt ttaattagat tatgtacaaa atataaataa caatttaaat aactttttaa
    18421 tatattgtag tttattatat aaactttata ttataatgta taatgtggag ttgattataa
    18481 aattgatata actcaagtca tattaaaaga ggtgtatttt atactatgga tttattgcat
    18541 cttaagtatt ttcaaactgt tgctagaatg gagcacataa caaaagcatc acacaagtta
    18601 aatatagcac agccagcttt aagcaaaact atatcttcct tagaaaaaga acttggagtt
    18661 cagttatttg acagaaaagg aagatatatt gttttaaatg agtatggaag attgtttcta
    18721 aaaagagtag atagcatttt agatttagta gaaagttcaa aaaaggagtt acaagacact
    18781 tctttagaaa attcaggaga agtaaagata ttatctcctg cagcggctaa tgtgttacca
    18841 tcacttttga gtaatttcag aaaactatat cccaatatta cttttaatgt gtcacatact
    18901 ttgccatcat cttataaaaa aagtgatttt gatttgtaca tatcttcttc atttacaaag
    18961 ctaaactcag aaaatagcat aactttaaca tgcgaagaaa tactattagg agtttcaata
    19021 aaccatcctt tatcattaaa ggatgaagta tatctaagcg aagtttcaga tgaaaacttt
    19081 atagttataa cgaagggtga gaattataga gaggtaattg atatcctgtg cgaatcagct
    19141 aatttcaaac ccaaaatagc atttgagtct gattcaccat atacaattta tgctcttata
    19201 aaatcacttc aaggagttgg atttatatgt ggaaaatcat ggggattatc tcaagaccct
    19261 gagattaagt tgttacatat caaagatatt gaatttaaaa gatatttaaa tctttcatgg
    19321 tttagcgaaa actatgaatc taaagctgtg ttgttgttca aaaactttct tatcaactat
    19381 tttaaaaata tttaaatcaa agttgataat attaaagagg atatatcaaa gtataataaa
    19441 tttaatctta atattgatac atcctctttt tatcatttta tattgaactt gtattatagt
    19501 atagaattat atgttatagc tactttttct gtctttcaaa taggggatat cttccctata
    19561 cttttttatt tcatctaaat caatctctga taatacatat ttttccctgt atgtatctaa
    19621 ttgagttatt acagtacctc taggattgca tattttactc tttccaaata gtattaaatt
    19681 agagtcatta cttactaaat ttacacctac tgtatataca gtgttctcta aagctctttg
    19741 agatacattt aaatcccaca tatcttcatc ttgtattctc catgcagatg gtataaatat
    19801 tatttcagaa ccttttaatg tcaattctct ggatacttct ggaaaacctg catcataaca
    19861 tatcattaca ccaaatctac caaaatcagc atcatatacc tctacttttt cccctccttt
    19921 aaagtatact gcttctaatg accacagatg atttttacag tattctccca taatcttacc
    19981 ttttctatca aatataactg ctgaattata aacatgtgta gacccttttt ctaaggttcc
    20041 atatggagat atcaggtata cattatttct tttagcagct tctgacattg attcctctat
    20101 gtacttatta tgttctctta ttaattctaa tgttttaact ccacctaaag actctaaatt
    20161 atatcctgta gcaaatagtt ctggtaaaca aattatatct gccccttgtt ttcctaagtc
    20221 atctatcatc tctactgctt tttctatatt ttttttaaca tttccaagta cactgtgttg
    20281 ttgaattata cctattttca ccttacgacc catgtaattg ccccctttat ttatctttga
    20341 ttccaaaaca attcattaat atataataaa taaccatact cactacaatt ccatttatcg
    20401 gaggtattaa gaaatttatt tttgaactat aatacgacgc tgcaaaacct attaaccaac
    20461 ttattactgc aatccaattt attccttctc tatgcttata agttcttcct tgtataaaat
    20521 aatactcaca ccacataatt cctgctattg caggaactgc gattcccaag aaagataaaa
    20581 atgactgaaa tttaaatatt ataccagtag ctgctattat tgtacctatt agacctgcaa
    20641 ctaaagtcaa aacatttctt tttacatttg gaaaaatagt ttttaatgct aatccaccag
    20701 aataagcatt tgatacgttt gtagaccaag atgcaagtat taaaataaca aacgctaccc
    20761 atccaaaacc taaattagcc aatacttctg ttatatccca agtaggtgct cccatagaca
    20821 ttacaccagc tgcaacttgt tggaataatg aaataatccc tagacctaaa aaactgataa
    20881 tccaaatgtc ttttactcgt ctcgtatatc ttaactggtc aggtgatata attgcaccaa
    20941 ctataacaag acctactaca atattaagac cttcaaccat actcatagtt gtttgaggag
    21001 tataattata aatagcttca actccataag tagaaaaagc tttaaaaact ccccacacca
    21061 ttaatatcat aagtggtggt ataactaagt aattaaattt agccatagct ttaaagccat
    21121 acagtgcaaa aacaaccata aataatccaa gtataactgt aactaatgtt aggtttacac
    21181 ttgggaatat cttttgtact gatagtcctg caacccctgc ttgtacacca aaccatccaa
    21241 aactagttat accaagtaat agagatataa gatactttcc tcctatctct ccaaatgccc
    21301 ccttagctaa tacatacgta tttaaccctt ctttgtaacc tatataacct tgtatcataa
    21361 taattgcaac taatattaag ttccctataa ttgatgtaat aatcatatta gaaagagtca
    21421 ttcctaaaat taaagttccc ccaaccatta gttcagatat agctatgtta gaaccaagca
    21481 tattaaacaa tggagctaac catccaactc tttcctcttt tggaactgga tttaaaacta
    21541 attcttctct ctcttgcaat attatccctc cctttcttta taaaagaata atttctactt
    21601 ttttctactt ttttttcact attaatagaa tttttcttta tataattgta attctattct
    21661 acaaaaaagt ataatatcta gtttgttatc aatttgatat aatttaagtt ataaatagat
    21721 aaatagtcca aatttattat tttaatttaa tctattaagt catatattag acttatttca
    21781 aatttgttat atattcttta attaaaatcg tgtactcatt acagaagtag tttctaaaac
    21841 aatcaagttt atgtaaaatt tttaaacata gtattttaat tatattggct gagaatacaa
    21901 agataattta tgaaaatata tagagaaaaa atataaaata attctaataa aaaaagtctg
    21961 aatataaata tactttatat ccagactttt tacattttag tttcttgcaa ctacatatta
    22021 ttcattatct tttatatcaa cttttatact taattcttct aattgctcat cttctgccaa
    22081 tgtaggagca tttgtcatag ggcaaacagc attttgattt ttagggaatg caataacttc
    22141 tcttatatta tcagttcctg caagtaacat aacaagtcta tcaagaccaa aagctagacc
    22201 agcatgagga ggagtaccat atttaaatgc ttctaataaa taaccaaact tttcattagc
    22261 tctttcctct gtaaaaccaa gtgctttaaa cattcttgat tgaacatcag aatttgatat
    22321 tctaacactt cctccaccta tttcataacc attcaataca atatcataag ctttcgctct
    22381 taaagatgac ttatcacctt cttctaactt gtctaaatct tcatccatag gtgatgtaaa
    22441 tgggtgatgc attgctgaga atgtgcctgt ttcttcatct tcttcaaaaa ctgggaactc
    22501 agttacccat agtaagttat atacattttt atccaataga ttaagcctat ttgctacttc
    22561 aagtctaact tgtcctaaag catcaaatac tattgagttt ttatcagcta caaataaaag
    22621 taaatctcct acttcagcat tcattcttgt aagtatagcg ttcatttctt cttcattaaa
    22681 gaattttgct attggagaag ttactccttc ttgtcctact ttcatccaag caagaccttt
    22741 tgctctatat gtcttagcat gttcttctag tgatgatatt tgttttctag taaagtcgtc
    22801 agctttgcct tttacgttta tacctctaac actttttcct ggttgtgtag catcagcaaa
    22861 cactttaaat ccacagttac aaacaacatc agatatatct gttaactcat atccaaatct
    22921 agtatctggt ttatctgaac catatttaga catagcttct gcataagtca tcttaggaag
    22981 tggaagtgcc aattctacat ctaaaacttc tttaaatatc gcttcaagca aaccttcaat
    23041 catactcata acatcttctt gctcaacaaa agacatttca cagtctattt gtgtaaactc
    23101 tggttgccta tctgctctta aatcttcatc tctaaagcat ttaactattt ggaaatatct
    23161 atccatacca cttaccatca ataattgttt atataattgt ggtgattgtg gtaatgcgta
    23221 aaattttcct ggattaactc tacttggtac tagataatct cttgctcctt ctggtgttgg
    23281 agctattaaa aatggtgttt ctatttcgca aaatctattg tttgataagt aatttcttgt
    23341 aatattcatt actttatgtc ttaattttaa atttctttgc atagatggtt ttcttaaatc
    23401 taagtatcta tattttaatc ttagtgcttc agaaacatca tcatcatctt ttatataaat
    23461 aggcggagtt tctgacttgt ttaaaagtct aagttcagtt gcaaatattt ctatatcacc
    23521 agttggcata ttagggtttt tagattgtct ttcgcatact tttcctttta cagctattac
    23581 aaattcagcc cctaattttt cagctttttc aaaagcttct ttatctacat ctgtatcaaa
    23641 aactatttga cataatccac ttgtatctct taaatcaaca aatacaagac cacctaaatt
    23701 tctttttttc tgtacccatc ccataagtac gacttcttca tttatgtttt tttctctcaa
    23761 ctctccacag tagtgagttc tctttaaacc ctttaaagtt tccaagatac tacctccatt
    23821 ttatttaatt ttaatgctat atatttatct aatacacaaa acatcatact ttcttatttt
    23881 acagcttttg ctttaattct tcaactaatg tacttaattt tacagtagtt tgctctgatg
    23941 ttttcatatt ttttaatgtc gcagtgtcat ttttaagctc atcatctcca attactattg
    24001 taaattttga atttatttta tctgaatatt taaattgtgc tttaacactt ctctctatat
    24061 ggtctttatc tgcacttata tgattttgtc tcaagtcttt taacaattta aagcttcttg
    24121 tatttgcttt ttcacctata gttactataa atatatccgt agattgaggg ttttctattt
    24181 caattccatt accttcaagt gtaagtaaaa gtctttcaat tcctagtcca aacccaattc
    24241 cacttatacc ttttggtcct cctaattgtt caacaagacc atcatatctt cctccaccac
    24301 atacagtact ttgagctcct atatcattag atataatttc aaaagcagtt tttctatagt
    24361 aatctaagcc tctaactatt gttttatcaa ttacaaagtt tatattcatt tcctttaagt
    24421 attcttgaag tttatcaaag tgagatttac aatcatcaca caaatggtct gcaataaatg
    24481 gtatatcttt gatattttct ttacatgtag gatttttaca gtctataacc ctcattggat
    24541 ttttttctct tctctcattg caagtatcac acaatatatc agcttttgag tcaagatact
    24601 cttttaataa agcattgtac tcttttctac aaactggaca gccaactgaa tttatactca
    24661 ctaccaaatc atttaatcca acttcattaa aaaattgaac agctagtgcg attatttcag
    24721 catccattga aggagtatca cttccaagtg cttctattcc aaattggtga aattgtcttt
    24781 gtctaccagc ttgtggtctt tcatatctaa aacaaggtgt tacataaaat aattttgttg
    24841 gttgagtatc tgcatataat ttattttcta taaatgctct tactacacct gctgtacctt
    24901 ctggctttag ggtaatttct ctatcacctt tatctttaaa agaatacatt tctttttgaa
    24961 ctatatctgt agtatctcca acacttctct tgaaaagttc tgtatgttca aatataggtg
    25021 ttaccatttc ttcatatcca tataaagcac atatttctct aaatttattt tctacataac
    25081 gccatttgta agcctctttt ggagttatgt ctttagttcc tctaggagct ttagtcaaca
    25141 tttttatata cctcttcttt cttccttttt atcatttcat ctactctttc aatgtattgt
    25201 tcaacatttt ttacattttc tactctttca attctatctt catctacata aacaaatttc
    25261 gatatagcac ctgcacctaa tgcatagtta ctttgttttt cttccataat ctgtatatta
    25321 taaatgcact catatccttc tttagcataa cctatatttt ctaaatttcc caacatatgt
    25381 ttttgtctat acatataata tggatttaat cccatatctt ttgcatattt cattgataaa
    25441 tctatcattt ttaccatttc ttcatattgt gttagatgtt ctttatacct atccatattt
    25501 atatttaagt tggatgctct ctttatggct aatgtatgaa ctgtaaggct ttcaggagaa
    25561 agttttttta tctcctccaa tgtattcttt accatattta aatcttcatc tataagacct
    25621 aaaattatat ccatatttat attatcaaat cccattctac gagccatatt aaagcaatct
    25681 actaaatcag atactctatg ctctcttcca atctttacca aggtatcatc attcatagtt
    25741 tgaggattta tactaattct actaacattg tgttttttta acactcttag tttttgttct
    25801 gtaatagtgt caggtctacc tgcttctaca gtaaattctt taattttact taaatctaat
    25861 tcattgaaca atgaatttat aagcaaatct aattcttcct catcaagtgt agttggagtt
    25921 ccaccaccaa tatatagagt ttcaacttct ttcttagtct ccttaataac tttggcaaac
    25981 cccttaattt cttcaatcaa tttataaaca tattctcttt ttaaatgtcc aaattgtttt
    26041 aatgaatttg aaggaaatga acaatataca catctagttg gacaaaatgg aatacttaca
    26101 taaagagaaa ttttattttt atctatagga tacataaata atcgctctct tttagctatt
    26161 tcaagagcta aatctatttt ctcatctatt ataaaataat tatcttttag aatggttctt
    26221 atgctattat catctagctt tttatccatt aaattgtgga caattttgac tggtctaata
    26281 ccagtcaaaa ttccccatgg tacataagaa ttaaatactt gtttaagcac tacaaacatg
    26341 ctttttttga tattttcttt acacagcttt ttaaagttcc tcttactttc actatctagg
    26401 ctattaagaa attcttcaat cgttttgctg ctatcaaatc catctaaaaa ttcaagattc
    26461 atattttcta tattctgtat tgactcgtac tttaagtttc cattttcata gtattcagta
    26521 cttgagaata atacaccatt ctcataaatc aatctattta ttaaatgctt tgactctata
    26581 ttattgttag cattagagtc tttaaactta aattcactag taaacaattt tataagttct
    26641 gctacttcat acttataatc atgtcctttt aaaaaaacat acaacatcaa cttaactcct
    26701 tctatttaac agtagaagaa ccccctcact aatatagtta ttaaatattt tatacttttt
    26761 taaatactaa tataaaatta catgtaagga tttgtcttct tttcaacccc tagagtactg
    26821 gttggtccat gtccaggata tactctaaca gcatcttcat atttacataa tttcttaagt
    26881 gatttttcca tttggttaaa gtctccacta tataaatcag ttctaccaat acttccagca
    26941 aacaaagtat ctcctgtgaa catttcatct tcaactctta tacacataca tccttgagta
    27001 tgtcctggag tatgcaagaa agtcattttt aattcaccaa gttcaagctt gtctttatca
    27061 tttacataga tatctgcatc aaactcttgt gctccacatc tcattgtata acttagattt
    27121 ttatttttgt catttaataa ttctttttca tcaacatgtg caactacttt agcaccagtt
    27181 ttttctttaa tatccttaac atatcctata tggtctccat gcccatgagt aaggataata
    27241 tattcaatat ttaattcatt ttcttttaca aaacttaatg tctctactaa acttccacct
    27301 gggtctataa ctgcacattt tttagtcttt tcatcaccta atacataagt atttactcca
    27361 aaataactat ctacaaattt ttttataatc attaaagttt tcccccttaa aaagatttac
    27421 ttgagtctaa tagtattgtt actggaccat cattagttaa ctctaccatc atatgagctg
    27481 caaattgacc tgtaccaact gtcacacctt tatcttttgc tttttttaca aattcttcat
    27541 aaaggttatt tgcaagttct ggtctagctg cattactaaa tccaggtctt cttccttttc
    27601 tgcaatctcc atataaagta aattgagata ctactaaaag ctctccacct atatccatta
    27661 gagataaatt catcttgtca ttttcatctt caaaaattct cagattcaat attttatcaa
    27721 tcatataatc tacatcactt gatttatcat catgagtcac tcctaacaat actagtaacc
    27781 ccttatttat ttgacctata gtgttttcat ctactgtaac tttagatgaa gatactcttt
    27841 gcactactgc tctcatagtt tcctccctat tattttaact ctaatttgtc attctataaa
    27901 tactttctac accaggaatt gcttttaatt ttttcattag aatttttaat tcctctgtgt
    27961 cattaacctc tacaagtaag tttatactta caatactatc cttacctttt cttgcattta
    28021 ttccgtttaa actaagttta tccattgcaa caatatgagt tatttcatta acaattcctc
    28081 ttctgtcaat agcttttatt tgaatttccg cttcaaactt accttttcct gctgaactcc
    28141 atgaaacctc caccaatcta ttttggaaga aatcaccatt attgtttgca tttgggcaat
    28201 cagctctgtg tatggctact cccctaccct tagtaatata tcctacaatt tcatctcctg
    28261 gaagaggatt acaacactta gcaaatctta ctaagatatt gtcaagccct tttactataa
    28321 ttccttgtga tgcgtttttc tttctctttt tactgtattc tgtttcactt atattgtgtt
    28381 tatttaaatc ttcttcttgt atctgttgtt cctttttagc ttttttaacc tcttttaaat
    28441 ataggtcttt taccttagat accacttgag atgacattat accaccataa cctacagtgg
    28501 caattaagtc ctcaacactt ggctgattaa atttcttagc aactaatgcc atatattttt
    28561 caacaagagg gtctttttgt ggtatatcat gcttcttaaa ttctctttct aatattgtat
    28621 ttccacggtc aacattttct tctcttcttt ccttcttgaa aaattgtcta attctacttt
    28681 ttgcatttgg ggtcttcact atattaagcc aatccctact tggtccatta gaattagatg
    28741 atgtaagtac ctcgacaata ttaccatttt gaagcttata atcaatagga actattcttc
    28801 catcaatttt agctcctaca catttattac ctacatttgt atgaacccta taggcaaaat
    28861 ctataggtgt tgattctgct ggtaattcaa ttacgtctcc ttttggcgta aatacataaa
    28921 cttgactatt aaatacatct tctttaagtg catccataaa ctcttgtggg tcttttaaat
    28981 ctttttccca ctccatcatc tgtctcaacc attgaagttt ttcttcaacc tttgattctt
    29041 tgctagagat accttcttta tatttccaat gggctgctat accattttca gctatattat
    29101 gcatctcatg agttcttatt tgtatctcta aaggttctcc atcaggacct acaacagtgg
    29161 tgtgtaatga ttgatacata ttgggttttg gcattgctat ataatcttta aatcttcctg
    29221 gaataggtct ccataatgta tgaactatac caagtactgc ataacaatcc tttacattat
    29281 caaccaatat ccttactgct gttaaatcaa aaatctcttc aaaagtctta tgtttttttt
    29341 gcatctttct atatatactg taaaaatgct ttggtctacc atatacatca catggtatac
    29401 ttaaatcttt aaacttatct ttaagaagtt caacaattcc ttgaatataa tcctctcttt
    29461 gacttctctt cattgatact ctactaacta aatcataata cccttctggg tccataaatc
    29521 ttaatgctct gtcttctagt tcccacttta tcttggaaat tcctaatcta tgagctatac
    29581 caccataaat ttcaagtgtc tcttttgctt tatactttgc tttttctgga ggcatgtatt
    29641 ttaatgttct catgttgtgc aatctatcag caagttttat aagtataacc cttatatcct
    29701 tcgccattgc caaaaacatt tttcttaaat tttcagattg agtttcttct ttcgaacgat
    29761 attttatttg accaagttta gttactccat caaccaaatc cgctacttct ttgccaaatt
    29821 ctttttttat gtcttcatag gtatattctg tatcttctac aacatcatga agtaaacctg
    29881 ctgttatagt ttcaatatct aattccatct ctattaaaat atttgcaact gctaatggat
    29941 gaataaagta tggttcccca gattttctat attgtccttc atgcgctttt tttgcaagat
    30001 tataggcttt ttcaaccatt tcaaaatttg cattaggtgc ataaactttt attttttcaa
    30061 ttatttcttg taattcttta tcatgcatat aaccacctat tttgtataaa attttaatat
    30121 atatgaaaat agttggtgtt ataccaacta ttataaaagt actagtattt tataagagaa
    30181 ttaacatcat agttagacaa cttttctctc ccatttaaga attttagctc aatcaaaaat
    30241 tgcatagaaa ctacttctcc acctaatttt tctacaagct tggcagcggc ttccatagtt
    30301 ccaccagtag ccaataaatc atctactatt gctacttttt gacccttttt aattgcatcc
    30361 ttatgtatct ctaaagtatc tgtaccatac tctaatccat actcataact ctcaacttca
    30421 cctggtaact ttccaggttt tctaattgga acaaatccta ctcctaaagc atatgctaca
    30481 ggtgtaccca tcaaaaatcc tcttgcttct ggtcctatta ctaaatctac atctttatct
    30541 ttcaattctt caactataga atccactgca tatttaaatg catctccatc tttaaataga
    30601 gttgtcacat ctttaaaatc tatgcctggc tttgggaaat catcaatatt ccttataaag
    30661 ttttttaaat ccatgggaaa cgcctcctaa taactgctat tgtattgttt ttctaaattt
    30721 tttagattat tgagtattaa actctcattt aagtctaatt ttttctgtgg ttttggcaat
    30781 attcttatga gtacagaatt tttttcataa tttaaattaa agtcaagtaa atttaattct
    30841 ctaaatacat ttaatatcgt aaaaaacttt aatggaatta cattaaaagt ttcccttata
    30901 tttaccatat ccaaatcaat ttcttttttt atgagtgctt gtttataaat tgttataaat
    30961 tcatctcttg atggaactat attactaact acattcttta aatataatag gtctgtttta
    31021 ttataatatt ttataatttc actttcttct cttttattct catatatata cgaatattcg
    31081 cctttattgt ataaataatc ataaagaata atattattat atctttttaa gtctatttta
    31141 tcaatattag gagaaaatat aagctctaat ttatcagtat ttttgctaac atcttcaata
    31201 atattgtaat ttatgttaaa atccaaatct ataagtgata tatctgacat tgctctataa
    31261 aatccattta tagaattaga tattattagt gtatcttttt caaaataatc aaatatattt
    31321 atatctgtat ttccatcaaa taattcaaaa tcagacacag atatattata caggctttga
    31381 gtatcttttg gtattatttt tgacattaat ttcaatgaaa gtttgtcatt acttgcactt
    31441 ttaggtcgag ctaatcgtat atctttaagt aaaaattgta ctttcctatt tcccatatag
    31501 ttattttcat ctaattggaa tagtacatct actttgtctc ctactttata cattgatttt
    31561 aaatgactca tattaaatcc tatacattca tagcttttct ctttttctat acttaatttc
    31621 agatgttgtt gattcttacc tataactttc aaatctttta aaatataatt tctaactata
    31681 aatctaggat taggattatt taatccaaag ggttctaact tatgtaattc ttctacaaga
    31741 tttaaattta ttacattttc ttgtaattca aactcgacat ttacattctc tatcatatca
    31801 tcttcagtta aattataatc tgcaaattta tttatttcat ttgctaaaat ttctacattg
    31861 tctgaatcca aggataaccc tgctgcttgt tcatgaccac caaacttagt catcaagtct
    31921 ttgcacttta tgagagcttc aaatatatta aatcctttta ttgacctcgc tgaaccagta
    31981 gcctccccat tttctatacc tagaagaatt gttggtttgt agtatttttc agttaacttt
    32041 gatgctacaa ttcctattat tccatgttgc caaccctctt tagctaaaac caacaccttg
    32101 tcatcattgt atctgctatt agactttagc atttcctcag cttcatgata catcttagct
    32161 tctatcatct gtctttcatt atttttttct tccaatatac tagctatttc tactgcctct
    32221 tcttgtgatt gagtagtaaa tagctctact cctaagtaag aatatcccaa tcgacctgaa
    32281 gcatttatcc taggacctat tgcaaaacct atatgagaag aacctatctt atcactttct
    32341 acaccacaaa cttttataag ctcccttaat ccaatatttt tgccttcttt cattgatttt
    32401 aaaccatttt ttacaattat tctattttca tctataagtg gaacaatatc acaaatagtt
    32461 gctagtgtta ctatttccaa ataattatac atactagttt taaattcttc ttttggagtc
    32521 aaggcttgaa tcattttaaa tgctacacca catccacata gtgaatcaaa tggataatta
    32581 caatcttctt gttttggatt tattactgca aaggctgatg gaatttcact ttgacattca
    32641 tgatggtctg taattataac atctatgccc aaatcattcg ctgtattgac ctcagacact
    32701 gatgtaattc cacagtcaac acttataatc aagtcacatc ccctagagct tataagctta
    32761 atagcatctt catttatacc atacccttct tctaatctat taggaatata gtaatcaaca
    32821 ttgtaaccta tactcttaaa atataagcac aagatagatg tagatgatac accatcaaca
    32881 tcataatctc cgtaaatcca aattttttcg ttcttctcaa ctgctaactt tattctatca
    32941 acagcttttt gcatatcttt cattaaaaaa gggtctctta aataatctaa agatggattc
    33001 ataaatatct ctgaatcttt ttcagtacat atccctctat tttttaaaat ttggcagatt
    33061 tccggggaaa tattaagttt tttgctaaat tcactttcct ctatcttacc tttatacttc
    33121 aatatccatt ttttcaaatt taattccccc taatcttttt tgtattttga cctaatactt
    33181 cttataacta agcaaaggcc tattataaag gatataaaga aacccgctat tgcagtaaat
    33241 tttatccata tattattttc tatatttgga gatgcaatta taagagaaga acctactata
    33301 atagatgcca acactaaact caaagatatt tgagatgtta gctctattat acaattttct
    33361 aacctcgtca ttttaacatc ttcaatttgc atttttatat tatttctttc tatatttttt
    33421 aaaattgctt ttaattgctt tggaatagtt tttatgtcca gtaatatttc ttctacattt
    33481 tgtcttgaac ttaaaacaac atttttaggg ttaaatttac tcttataatg atgtttcata
    33541 aactcttttc ccatagaacc aaaagaaaaa tctgtgttta aagttctact tgttccttct
    33601 aatgttataa ctgtctttgc aagagttaca aattgtgcag gcatgactat tttatactgt
    33661 ctaaaaaacc taaatagttc atttaatata tctgttacat ttattttttc tatagaaata
    33721 tcatagtaat aatgtattag ataaagtaaa tcctgtctaa gtcccttaat atcagcctct
    33781 ccatttaatg catccatttc tatcaataga tatattatct tgtcaacatt cttttctaca
    33841 cctgctaatg caatttcatt taattggttt aaagttttat tatcaataat tccaatcatt
    33901 ccaaaatcta tatatgctat cttattttta gcaattacga atatattacc tggatgtggg
    33961 tctgcatgaa aaaatccatg agataatacc tgtgtaaaaa atgacctaac gcctatttca
    34021 acaatagttt ttgtgttata acctagtctc cttatttttt ctacatcact taactttgta
    34081 ccattaactt tttccattac taaaattttc ttagtattat attctgaata tactttaggt
    34141 atatatactt catcactatt tttaaatatc ctagagaatt tagttgcatt tatagcttca
    34201 aatgtataat caagttccct cattaattga gtttgaaatt cttcaatcat ctgtactaaa
    34261 tctaaattaa aatccttttt taaatctttt aaagtatttg caatagttct aagaatctct
    34321 aaatcagatt ttattatttt ctctatattt ggtctttgaa tttttacgat tacttcttct
    34381 cccgttttaa gtacaccttc ataaacttga ccaattgaag ctgctgctat tggttcttct
    34441 ttaaattcta agaatatttc ttctatactt aaacccactt cttctttaaa tatatcaata
    34501 gctatatttg aatcaaattt ctctacatca tctctgagtt ttgatatttc atcaattata
    34561 tcctggtcta acaagtcttt tcttgtactc aatatctgtc ctatttttat atatgtaggt
    34621 cctagttctt ctaaaactct tttcattctc tcaccagttg tcatattttt tatttcttct
    34681 ggtgggtcaa atagtggtat tttataggca attccttcta tatttagtct ttctacaata
    34741 aaactaaaac catactttat taaaacatat ccaatttctc tatatcgctt taaatttcta
    34801 taacttattc tcaaatcttt tcactcctaa gataggtata cccaaaaact tacaccttaa
    34861 tttatcgtat atgacattat ttcaccaatt gatattctaa tatcctatat cctctttaat
    34921 atttaatttt atcacatata ctaccaattt tattaaaatt atttaactca gttaattttt
    34981 atttttgaca ttttatatct ggtttaatca aatacttttt gaaaactaaa atatcaacaa
    35041 aatctaataa aataaattag tggttgcctc aaaacagatt taaatatgtt ttgaaacaac
    35101 cactctttaa actaatattt ttgattttgt gctgcctcta aataccattc ataagattca
    35161 tcaatatatt ttttatcttc ttctgtaagc tctctaacaa ccttaccagg agaacccatt
    35221 atcaaaacac ctggtggaaa cttcttatta gatgtaatta gtgtacctgc tcctatcaga
    35281 gtatattctc ctatttcagc attatccaat attattgaac ccataccaat taacacatta
    35341 tcactaattt tacaaccatg aactatagac ctatgcccaa ctgttacatt gttaccaatt
    35401 atagtttctg tatctccatg cactatagaa caatcttgta tattagtgtt ttctccaata
    35461 gttattggtc cttcatcacc cctaactact gcattgtacc atatacttga atctttgcct
    35521 attttaacat ttccaattac atctgctgat tttgccacaa atacactttc gtcaattaaa
    35581 ggtttatctt ctaaataatc tctaatcata tataaacaac tcctttaaaa tataaattta
    35641 gttggttgct ccttctaaca ttaacttagc ttgtatcatt atagagcact ctgtacgttt
    35701 tctttgcatt tcacagccta attcatatgg ttttgatatg tcattattaa agtttatcgc
    35761 atttgcatga catccacctg aacagtaaaa cttattccaa cattgtttac actcttcttt
    35821 agtatataca tgagcttctc taaacatatt ttttaaattt tctggaagta ctatttcttc
    35881 atcaaatata tttgccattt taaattcttc atttccaaca aattgatgac atggatagat
    35941 atctccattt ggagttacag aaagatactc atttccagct ccacatccag ttattctctt
    36001 tattacacat ggaccttgat ttaggtctat cataaaatgg aaaaatttaa atccatctcc
    36061 ttgtaattgt ctatctgcat actctacagc aaatttttcg tactcttcaa atatcttagg
    36121 taaatcttct tctctaagtg catatggatt actctcatct ccaacgactg gctctacaga
    36181 ggttaattta aatcctaaat ctgcaaaatg cataacatct tttgaaaaat ctaaattatc
    36241 ccttgtaaaa gtacctctta tataataata cttatctttt gctctttttt ccacaagttt
    36301 cttaaatcgt ggtaatgtaa tatcataact tcctttatca tttaaagttg gtctcatatt
    36361 atcattaact tcttttcttc catctaaact taaaactaca ttatgcatat tttcatttat
    36421 ataatctata atctcatcat ttaacagtac accatttgta gttattgtaa atcttatatt
    36481 tttattataa tctttttcta cacttcttcc atagtcaacc agttgtttta caacttcaaa
    36541 gttcatcaaa ggctctccac caaagaaatc tatctctagg ttctttctac ttcctgaatt
    36601 agcaatcaaa tagtcaattg ctgccttacc aacttcaaaa ctcataagtt ctttttctcc
    36661 tccaaaatca ccttgagctg caaaacagta tttacatttt aagttacaat catgagctac
    36721 atttaaacat aaagctttta ctacaggctc tctatgtaca aagcttggat gatattgata
    36781 tgtatcttct gtatataaaa gaccctcttt ttccaaattt aatatttctt catatgcttc
    36841 atttattttt tcttcttgat attttgattt taatatctct aatatttctt ctttactttt
    36901 ttctttataa aaatctaaca aatcgtatgc aacatcatct aacacatgaa cagctccacc
    36961 attcacatct aaaacgatat tatatccatt catagaaaac ttatgtatca tactcatact
    37021 ctacctccta aattactatc tatactaaag ttttttatta ttttaattaa actttaccct
    37081 tcattatatc ataagtttac ataaaataca tacaatacaa aaatgactta ctctccgtaa
    37141 gtcatttcat taaatacact atatccccct aaattggcgc actaatatat taccaaatag
    37201 gtattaggct cctataaaat aaataaaata aattttgtat ctgattttat attgttttaa
    37261 tcttattaaa atataataat ttcttttttc ctgataattt tgtataaata agtgcttgta
    37321 tttaatttgt cttctttaga ttgctgattt ctttgggtac tttaggctga tgaataatcc
    37381 aaggacaggt actattagct gacaaaatag cagtgctaag tgccaatgag ctagcaaact
    37441 tcataaatct tacaataaac tttttcataa cttaactctc cctcatacat attttactta
    37501 tactttagct tttaacttac ctaatactaa catcacaaaa atccaaaaca tggcaaatgc
    37561 gctatatgtg aaataatcta caaatatact caaagaaaga cttaaaattg ttatcaataa
    37621 cactactgta gatataaata tagcagtttt cttataaacc aacttttccc tatcacttat
    37681 agggttactc ctatgttcta ttgggcacag aacacaaatt ccaacccaac taacacttgc
    37741 aataatcata ataatatttt taaataagtc tatatttata ttttctataa ttaagattgt
    37801 ggataaaatt ataaaaataa aggttaataa gcatctaaaa taattgtctg catgataccc
    37861 tcctgaaaat tgtcttattg gacaataaca cattaaaaaa aataatacat atgtaaattt
    37921 accaaataaa aaacctataa acagcatggt ggttatatta actatgaaag ctattaatat
    37981 ttcaaatcca taagaataga ctttagattc attattatct atcatattat tacaaattaa
    38041 aacagatgtc attttttcag catatctttt aaacattttt acctccaaaa aaattttaca
    38101 tatatagagt acattctttt ttgagaaaat tcaatatact ttacaaaact agttgttaaa
    38161 aatgtaaaac tggtcaaaaa aagacctata taattttctt cttattattt atataacaaa
    38221 acaattataa taaaacaatt ttatcaagct ttatatgcat aaaaagccta tgtcaaaata
    38281 gaaataaacc ttttccccat tgttaaaagt ctaaaaatat attttatttc atataaaaag
    38341 agtacctcac aaactaaaaa gttccttttg agatacccat tttatttatt acaaaatatc
    38401 aaattttatt tatttgcata tattttctat atattttagc cttttaactt tgtttttgtt
    38461 ttacatactg tagtgctttt ctattctgtt ttactgcttc aagacaaact ttttctgttt
    38521 gattatcaac aaattctaaa gctctataat tttgctttac tgcttctaaa catatttttt
    38581 ctgtttgatt atttacatat ttcaacattc tataatcttg ttttactgct tttagacaaa
    38641 cttcttctgt ttgttctttc acatatttaa gcgcttcata actttgatta actgcttcta
    38701 aacaaatctt ttctgtttgt tcctttacat aagctaaagc attatagttt tctcttactg
    38761 cctctaaaca tattaattct gtctgatttt ctacatattt aagaattttt ccattttgct
    38821 ttactgcttc tacaaatatt ctttctgttg gtatctttac ataacttaat atttctttta
    38881 tttcaagttc ttcaaatgaa cctcttactg cttctataca tatattttca gtctgttttt
    38941 ttacatattg cagtactttt ccattttgtc taactgactc caagcaaatt tcttctgttt
    39001 gctcttttac gtactgtaat gctcttccat caattcttac tgcttccaaa caaatctctt
    39061 ctgtttgctt atttacatac tgtagagcca tataacttct ttttactgct tccatacata
    39121 ttttttctgt ctgttcattt acataataca atgcaaagtc attctgtttt actgcttcta
    39181 tacatatttc ttcagtttgc tcttttacac aagatagtgc actgtagttt tcttttacag
    39241 cttctaaaca tatactatta ctttgttcct ttacataagc taaagatttt ccatcttttt
    39301 ttacagcttc taaacatatg gctggagttt gctcttttat tccttttaat atctctgcaa
    39361 catcattttt agatgccctt tctataaatg ctagacatac atcttctgtt tgctcttcca
    39421 caaatttaaa tacttccatg acatcataag ttgatgcatt ttttatagcc tcaattagta
    39481 cattttctgt cttattattt atgtagtaca aagctctgta gttctgtttt actgcttcta
    39541 tacaaatatc ctcattttga tttgttatat acttaagaga tttatatgtc tgttttactg
    39601 cttccataca tactttataa gtcttgtcct ttacaaactc aattatagta ccatcctgct
    39661 ttactgctag cagacatatc tcttctgttt ggttttctat gtcttttaac tctctgtaat
    39721 catattttac tctacttaaa tcatttaaat gtactttgtt atcttccttt aaatcttctt
    39781 cattattttc tatatcatac tcaaattctt catcatctaa atagtcattt ttttctggta
    39841 aaaatataga tttaatttta ttctttatat ccataaaatt atcctccttt aatatgattc
    39901 tatcattaaa gtaaatcgtt tttaagcact tatttaatta attttatatt aagtttattt
    39961 aaaagtctat tcattgtcga tagattatat tttatattaa taattttatc ttatatatgt
    40021 ggggtaataa agttaatagt ggacattttc catccc
//
LOCUS       DAEOSP010000015        38120 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.015, whole genome shotgun sequence.
ACCESSION   DAEOSP010000015 DAEOSP010000000
VERSION     DAEOSP010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38120
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.015"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            32..1144
                     /locus_tag="KRH54_000601"
     CDS             32..1144
                     /locus_tag="KRH54_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBG8578274.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLAYLLLKKLNVGSTATIITFLYIATFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENL
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            1398..3422
                     /locus_tag="KRH54_000602"
     CDS             1398..3422
                     /locus_tag="KRH54_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG8578275.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHNTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3642..4328
                     /locus_tag="KRH54_000603"
     CDS             3642..4328
                     /locus_tag="KRH54_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBG8578276.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4423..5478
                     /locus_tag="KRH54_000604"
     CDS             4423..5478
                     /locus_tag="KRH54_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBG8578277.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5593..7149
                     /gene="murJ"
                     /locus_tag="KRH54_000605"
     CDS             5593..7149
                     /gene="murJ"
                     /locus_tag="KRH54_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG8578278.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            7195..8901
                     /locus_tag="KRH54_000606"
     CDS             7195..8901
                     /locus_tag="KRH54_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBG8578279.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8923..9996
                     /locus_tag="KRH54_000607"
     CDS             8923..9996
                     /locus_tag="KRH54_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBG8578280.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10008..10730
                     /locus_tag="KRH54_000608"
     CDS             10008..10730
                     /locus_tag="KRH54_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG8578281.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LVCGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10746..11903
                     /locus_tag="KRH54_000609"
     CDS             10746..11903
                     /locus_tag="KRH54_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBG8578282.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRILLFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPVILTLFSVIIGTVFDKSTLLNRLLASRPKFWNVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11906..12745
                     /locus_tag="KRH54_000610"
     CDS             11906..12745
                     /locus_tag="KRH54_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG8578283.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12760..13935
                     /locus_tag="KRH54_000611"
     CDS             12760..13935
                     /locus_tag="KRH54_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBG8578284.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13964..14713
                     /locus_tag="KRH54_000612"
     CDS             13964..14713
                     /locus_tag="KRH54_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG8578285.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14735..15643
                     /locus_tag="KRH54_000613"
     CDS             14735..15643
                     /locus_tag="KRH54_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG8578286.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15691..16443
                     /locus_tag="KRH54_000614"
     CDS             15691..16443
                     /locus_tag="KRH54_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG8578287.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16397..17764
                     /locus_tag="KRH54_000615"
     CDS             16397..17764
                     /locus_tag="KRH54_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBG8578288.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            17770..18861
                     /locus_tag="KRH54_000616"
     CDS             17770..18861
                     /locus_tag="KRH54_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG8578289.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18945..20858
                     /locus_tag="KRH54_000617"
     CDS             18945..20858
                     /locus_tag="KRH54_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG8578290.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKDIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21057..21764
                     /locus_tag="KRH54_000618"
     CDS             21057..21764
                     /locus_tag="KRH54_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG8578291.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYAKRLITATRVLQ"
     gene            22066..24177
                     /locus_tag="KRH54_000619"
     CDS             22066..24177
                     /locus_tag="KRH54_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBG8578292.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24375..25340)
                     /locus_tag="KRH54_000620"
     CDS             complement(24375..25340)
                     /locus_tag="KRH54_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG8578293.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25625..26581)
                     /locus_tag="KRH54_000621"
     CDS             complement(25625..26581)
                     /locus_tag="KRH54_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBG8578294.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26949..27737
                     /locus_tag="KRH54_000622"
     CDS             26949..27737
                     /locus_tag="KRH54_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG8578295.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(27860..28330)
                     /locus_tag="KRH54_000623"
     CDS             complement(27860..28330)
                     /locus_tag="KRH54_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBG8578296.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            28469..29113
                     /gene="uppS"
                     /locus_tag="KRH54_000624"
     CDS             28469..29113
                     /gene="uppS"
                     /locus_tag="KRH54_000624"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBG8578297.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29156..30061
                     /locus_tag="KRH54_000625"
     CDS             29156..30061
                     /locus_tag="KRH54_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG8578298.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTNQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            30376..31923
                     /locus_tag="KRH54_000626"
     CDS             30376..31923
                     /locus_tag="KRH54_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG8578299.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            31926..32324
                     /locus_tag="KRH54_000627"
     CDS             31926..32324
                     /locus_tag="KRH54_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG8578300.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            32364..34097
                     /locus_tag="KRH54_000628"
     CDS             32364..34097
                     /locus_tag="KRH54_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBG8578301.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            34329..35540
                     /locus_tag="KRH54_000629"
     CDS             34329..35540
                     /locus_tag="KRH54_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG8578302.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIVQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            35733..35993
                     /locus_tag="KRH54_000630"
     CDS             35733..35993
                     /locus_tag="KRH54_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBG8578303.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            36347..38059
                     /gene="ptsP"
                     /locus_tag="KRH54_000631"
     CDS             36347..38059
                     /gene="ptsP"
                     /locus_tag="KRH54_000631"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBG8578304.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVFNMGTAEEIKSYIEK
                     TFNI"
BASE COUNT        15255 a         3987 c         6737 g        12141 t
ORIGIN      
        1 aatttcaaat aatgctgtag attctaaact tatgaatagt aggattaaat caatagatat
       61 aataagaggg ctatcaatag ccctcatgat agtctgcaat aatcctggga cttggatgag
      121 aatgtatcct caattaagac atgctgtatg gcatggtgta acacttgcag attttgcatt
      181 tccttttttt gttatatctt taggtgttac aattccaata tcaataaata gtaaactaaa
      241 aaataataaa tccacactga gtataatact tagtatattc aagagaagta tcttattgat
      301 attatttgga tttttcttaa attacctagg aaatcctgat ttagatactg taagaatact
      361 tggagtactt caacgtatgg gattagtcta ttttgtgact agtttagcat atttactgct
      421 aaaaaaatta aatgttggaa gtactgctac aataattact tttctatata tagctacatt
      481 tataatagtt gggtactaca tcttagcaaa accttatgga tttgagctag aaggttctct
      541 tgcacagtta gtggatttac atttcttcaa aggacatttg tacaagccgg aatttgaacc
      601 agatggtttt ttaactagta tagtagcaat ttcttcaggt atgttgggat gcactatggg
      661 atgtgtactt ttaaaagaaa acctaggaga atataaaaaa ttctttaaaa tactggttat
      721 gagcataatc ctgctaatag gtgcttttat ctttaatcag tattttccat ttaataaaag
      781 attatggtct agctcatttg tacttttaat ggctggcagt tatggtatat tactgtcaat
      841 attttatttt atatgcgaca tcaaaaataa aagtaaaata tttaccccaa taatagcact
      901 tggaagtagc cccattttta catatatgtg ccttgagata ttgagtcatg ttttttggaa
      961 tgttccaaaa cttacgaaca aagtcgatta tccaactacc ttggtagagt ggactacata
     1021 tgaacttata acaccttggg caggtacaac ttgggattct cttatatttt ctttattgta
     1081 tgtcctattc tgggttatag taatgtctat aatgtacaag aaaaaaatat ttattaaaat
     1141 ttagtgcaat atttttagat gataaagtct ttgatttgtt caaatataca gaaactatca
     1201 aatataatca aaatttctcc tcgataaaat ttaaaaacca tgccataaaa gattacaaaa
     1261 agtttacaat ttgtaatttt ttgtgctata atgaaaaaag atttacataa tattgaattt
     1321 atgtaatata aaacaagctt aagatgtatt aaaaaagatt taaagattta aagaaccaaa
     1381 aaattagggg ggatatgatg aaaaaaacaa caaaattatt ggcaacagga atgttgtctg
     1441 tagcaatggt ggcaccaaat gtagctttag cggctgaaaa tactactgca aatacagaat
     1501 ctaattcaga tattaatata aatttacaaa gaaaaagtgt agtgttagga agtaaatcta
     1561 atgcaagtgt aaaatttaaa gaaaaattaa atgctgattc aataacatta aattttatgt
     1621 gttatgatat gccacttgaa gctactttaa attacaatga aaagacagat tcatatgaag
     1681 gtgtaataaa ctataacaaa gacccagaat acttaaatgt atgggaactt caaagcataa
     1741 aaataaatgg taaagatgaa cagaaagttt taaacaaaga agacttagag tctatgggat
     1801 taaatcttaa ggattatgat gttacacaag agtttataat aagtgatgca aattcaacaa
     1861 aagcagtaaa tgaatatatg agaaaaactt ctgcaccagt aaaaaaactt gctggagcta
     1921 ctagatttga aactgctgtt gagataagta aacaaggttg gaaagatggg tctagtaagg
     1981 ttgtaatagt taatggagaa ttagcagcag atggaattac agctactcca cttgcatcta
     2041 catatgacgc accaatatta cttgcaaata aagatgatat accagaaagt acaaaagcag
     2101 agttaaaacg tctaaatcca agtgatgtaa taattatagg tgatgatggt tcagtttcac
     2161 aaaaggcagt gtctcaaatt aaatctgctg taaatgtaaa tgtgactcgt attggtggag
     2221 ttgatagaca tgaaacatct ttacttatag caaaagaaat agataagtat catgatgtta
     2281 ataaaatata catagcaaat ggatatgctg gtgaatatga tgctcttaat atttcatcaa
     2341 aagctggtga agaccaacaa cctataatat tagctaataa agattctgta ccacaaggta
     2401 catataattg gttatcttca caaggtttag aagaagctta ttacataggt ggtagtcaaa
     2461 gtttatcatc aaaaattata gaccaaatat ctaaaatagc aaaaaatggt acttcaaaaa
     2521 atagagtttc tggagcagat agacatgaaa caaatgcaaa tgtaattaag actttctatc
     2581 cagataaaga gttgagtgca atgttagttg caaaatctga cataatagtt gattcaataa
     2641 cagcaggacc tttagcagct aaattaaaag ctccaatact tataactcca aaaacttatg
     2701 tttctgcata ccataataca aatttatcag aaaaaacagc agaaacagta tatcaaattg
     2761 gtgatggaat gaaagatagt gttataaatt ctatagcatc tagtttatca aaacacaatg
     2821 caccaacaga accagataat tcaggttcag cagcaggaaa aactgtagtg atagacccag
     2881 gtcatggggg ttctgattca ggggcaacaa gtggattaaa tggtggagca caagaaaaga
     2941 aatatactct taatacagca ttagcaacta ctgaatattt acgttcaaaa ggtataaatg
     3001 ttgttatgac tagagataca gataaaacaa tggctttagg agaaagaaca gcattatcaa
     3061 atactataaa gccagattta tttacaagta tacattacaa tgcgtctaat ggagctggta
     3121 atggtgtgga aatttattac aaagtaaaag ataaaaatgg tggaacaact aagactgcag
     3181 catcaaatat cttaaaaaga atactagaaa aatttaacat gaaaaataga ggaatcaaga
     3241 caagaacact tgataatgga aaagattacc tatatgtgtt aagaaataac aattatccag
     3301 caatacttgt tgaatgtgca tttattgaca ataagagcga tatggataag ttaaatactg
     3361 cagaaaaagt aaaaacaatg ggaactcaaa ttggaatagg aatagaagat acagtaaaat
     3421 aaaatagaaa atattaaagc aaaacatatc atctcctgtt ataatgtaaa atataacaag
     3481 agatgatata tttttgtaaa gaatcaaatt gtttacaatg atgtttcttg tattgataat
     3541 ctgctcatta ttgagtataa tatagaaata agtcaaaaat aagatagaaa taagttgaaa
     3601 ttaagaatta aaaaaatttt tattccaata tggaggtaac tatgtctgga tatactaatg
     3661 atgagtgtga aatacctaaa attaaaagct atccaggggc agataaagaa attgcaagtg
     3721 agattgacta tagcatagta aaaggaacag ttttattcga cttgtaccaa agaattatgg
     3781 atttagtttt atctataata ggacttgtga taggtttacc tttaatagca atatttggaa
     3841 ttcttattaa aatagaggac aaaggaccaa taacttataa gcaagaaaga ctaggaaaat
     3901 gcgggagaag attctatata tataaattga gatctatgag aacagacgca gaaaaatttg
     3961 gcgcacaatg ggctgaaaaa gatgatccta gaataactaa ggttggaaag tttattagga
     4021 aaactagaat tgatgagatt ccacagctgt ttaatatatt aaagggggac atgggcttaa
     4081 taggtcctag accagagaga ccgaacttta ctgttcaatt taatgaggaa atacctggat
     4141 ttatcaacag gctcgctata aaacctgggc ttacaggttg ggcacaagta aatggtggat
     4201 acgaaatcac tcctgaagaa aaacttaaag aagatattta ctatataaaa aataggagta
     4261 tattacttga ctttaagata ctctttaaaa ctgttaaagt agtattgaca ggagacggag
     4321 ctagataatt ttaaaatatt agattactaa tcaatgaaaa agctctttaa aaagagcttt
     4381 tttattgtca aaaaatacaa aaaagaaaag gaatgagaac ttatgattaa aaaaatttct
     4441 actatattat cgttagtttt gttaatctct atttctagta cgataggagt atttgcagat
     4501 gcaaatccca aaagagaatt gatagaaggg agtataccag agatttcgac agaattaaat
     4561 aaaagagcat ttaaggattc taaagaagta attcttgtaa atgaagagtc aattgtagat
     4621 agtataagtg caacaccttt agcatatgca aaaaatgctc caatagttgt aactaagagt
     4681 aaaaatcttg gtagagtaac aagaaattat ctaaaagaac taggtccaga gaaggttacg
     4741 atagtaggtg gtttaaaggc agtatctaaa gatgctgaac gtaatataga aaaaatggga
     4801 atgaaagtag aaagaataag agggaaagac agatatgata catctttaaa gatagctaga
     4861 gaaatgtata gaacagtagg atttgatgaa gcatttttac ttagctcaac aacaggatta
     4921 gagaacgcca tatctgtata ttcttatgcc gctaaaagtg gaatgccaat aatatgggct
     4981 aaagatgaag ggtttgagga acaaattgat ttcttaaaag gcaaaaatct taaaaaaata
     5041 tatgcattag gtgattcaaa agagtttatt gcagagattg attctaactt aaaaaatata
     5101 gaaggaataa agcaaataaa taagtcaagt acaaatgttg atttaataaa gaagttttat
     5161 gatgaaaaag atataaagaa aatctatact gctagattag actttggtag tagatctgat
     5221 gtaaacgaat atatttcttt gggagtagta tcagcaaaag aaaatatgcc aatactgatt
     5281 tgtagtgata acttaagtcg tgcacaagat aaattcttga aagatagcaa tataaatgat
     5341 gtagttgaag ttgggtatac agtaggtgat tatagtctat ttaagagcat atttaatcta
     5401 acattcttat cttgtatagt attaatacta ttattactat tgattacatt tagggcactt
     5461 agatatgagt cgaaatagtt tttgatttat aaggaagctt ttcataggtt aaatttactt
     5521 aattagaata atgtttataa gcagttttaa aagttctact tttagagttt aaattagata
     5581 aggagaaaga agatgtcaaa aacagctaaa gcagcattgt ggattatggc agccactatg
     5641 ttttcaaaag tattaggttt ccttagagaa cttgtacttg caaatttcta tgggacaggt
     5701 atgtatgcag acgtatttgt attaacatta aatatacctg gtttaatcat agcagtcata
     5761 ggctctgcag ttgctacaac ttatatacct atgtactttg agacaaagaa aagactcgga
     5821 gatgaaggtg ccttaaaatt tactaataat gttttaaata tatgctatat aatggctata
     5881 gtaatagcta ttattggtct tttatttaca gagcaatttg ttacagtatt tgcagcagga
     5941 tttagaaacg accctgctaa gttccaagca gcaatattat ttactaaaat aatgatttca
     6001 ggagttttat tcctgagtgg aagtaagata tttagttcgt ttttacaggt gaatgacagt
     6061 tttgtaattc ctgggcttat aggaatccca tataatatta taataatagc agcaatagca
     6121 ctaagtgcag gtaaaaatgt atggatactg ccagcaggag cattattggc tatggcgagt
     6181 cagctattgt ttcaactgcc atttgcgttt aagaagtcct acaagtataa gccatatata
     6241 aatttaaagg atgagtctat aaaagagtta gtcaacctag tattgcctat gttggtagga
     6301 gtagctgttg gacagttaaa tatatttgtt gaccgattat tggcaaccac tttgggtgat
     6361 ggaaagttgt ctgctcttaa ctatgcaaat aggctaaatg agtttgttat ggcattattt
     6421 gttacatcta taataacagt tatatatcct aaactagcaa aaatgtctgg taaagataat
     6481 aaagaaggat ttataagcac tattgtaaaa tcttcaaatt gtattatatt ggtggtgctt
     6541 cctatatcta taggagctat tatattagca gagcctcttg ttagaatatt attccaaagg
     6601 ggtaaatttg atgcgttatc aacagacctt acatctatag cgcttagatt atattcacta
     6661 ggtttattgg cttgtggagt aagagatgtc ttatataggg cattttactc tctttcagat
     6721 acaaaaacac caatgataaa tggaagtata gctttaataa taaatatagt tcttaattta
     6781 atattgataa gaccattggg tcatgcaggt attgctatat caactagtac atcaaacata
     6841 attacagtta tattattatt tatatctctt aaaaagaaaa atggatattt tggaggggat
     6901 aaaattataa agactggtct aaaaagtttg gtagcatctg gtgtgatggc agtagctact
     6961 ttattgatat ataataatct atatgcattt atgggttcag gaacaattaa agaaattata
     7021 tcagttggag ctggagtatt aggtggagct tctgtatata ctgtattgat agttttattt
     7081 aaggtagaag agatggattt agcttttgaa tttttaagaa aaggaaaaca aaaactactt
     7141 agaagataga taattacctt gaaattttaa attagagtca gaaaggatga aaatatggat
     7201 tataagaata attatgagat gtggttaaat tctccttatt ttgatgaaca aacgaaaaat
     7261 gagctattaa gtataaaaga tgatgaaaaa gaaatacaag atagatttta taaaaattta
     7321 gagtttggaa caggtggttt aagaggtata ataggagcgg gaactaatag aatcaatatt
     7381 tatactgtta gacgtgcgac actgggcgtt ttaaattata tcatgaaaac tcaaggagaa
     7441 gaaggtaagc aaaagggaat agttatagca catgatagta gatacatgtc tagggagttt
     7501 tgtatagaag tagcaaagac tttaagtgct tatggtgtaa aggcttatat ttttgaagaa
     7561 ttaaagccta caccagaact ttcttttgct gttagatatt taaagtgtgc tatgggaata
     7621 gtaataacag caagtcataa tcctaaagaa tacaatggat ataaagtata tgactcagat
     7681 ggtggtcaaa tctgtataga tatggcaaat gatataatag cagaagttaa taagattgat
     7741 gattacagta ctattaaaag tatagatttt gaagaagctt tatctaaaaa tttaataact
     7801 atattagata atgaagtaga tgatgagttt ataaaagctg ttaagaaaca agttttaaga
     7861 caaaatataa tagatgaata cgggaaaaaa ttaaaaataa tatatacacc tattcatggt
     7921 acaggaaata agccagtaag aaaagtctta aatgaatgtg gttttgaaaa tgtaatggta
     7981 gttaaggagc aagaattgcc agattctaat ttttcaacag taaagtatcc taatcctgaa
     8041 gaaaagtcag tgtttaatat agctattgaa atggcaaaga ataatggcac tgatttgata
     8101 ataggaacag accctgattg tgatagagta ggtatcgttg taaaagattc aagtggtgaa
     8161 tatgtagtct taaatggaaa tcaagtcggt tcacttttag ttagatatat attagagagt
     8221 ttagttgaag aaaataaact tcctaaaaac aatcctacaa taataaaaac tatagttaca
     8281 tcagaactag gtgcaaaaat agcaaaggct tataatgtag attgtttaaa tactttaaca
     8341 ggatttaaat ttataggaga aaaaataaaa gcgtttgaag aaagcaatga tagaagtttt
     8401 ataatgggat atgaggaaag ctatggttac ttaattggaa ctcatgctag agataaggat
     8461 ggtgttgtat cttcacttat gatatgtgaa atggcagcat actattcttc aaaaggtatg
     8521 aacttgtatg aagctttgat agatacatat aataaatttg gatactacaa agaggactta
     8581 aagtcagtaa ctttaaaagg catagatgga ataaaaaaaa taaaagagat gatgctttac
     8641 tttagaagtg ttaaaataga taatgtggct gatgtaaaag tagataaaat tttagactat
     8701 aaagatggtg ttgatgattt acctaagtca gatgtactta aatttttact tgaagatggc
     8761 tcttggatag ctataagacc ttcaggtaca gaacctaaaa ttaaatttta ctttggagca
     8821 aattcagaca atcaagaaga tgtagaattt aagttgaaca atttaatatc ttatatatta
     8881 aatgtagtgg attctatata aaaagctaag gggagataaa caatgaaagt atacaatgtc
     8941 ataatggctg gaggaggagg aactcgtttc tggccactta gtagacaaga agtacctaaa
     9001 cagcttataa atttaagtgg agaagatgct ttaataaacg aaactataaa cagaatagat
     9061 tctctagcaa aaaaagatga tttatttata gtaaccaatg agaagcaatt ggaagcttta
     9121 aaggatatag taaaagataa atgtttggat agtaatatat tgccagagcc atgtgccaga
     9181 aatacagcag cagctatagg ctttgcagca tttaacataa tgaaaaaata tggtgatggt
     9241 gttatgtgtg tatatccagc tgaccattat ataaaagatg aaaaagaatt taaatctatt
     9301 ttggaaaaag ctatctatat agcagaaaat aatgataaac tagtaactat tgggataact
     9361 cctacatttc catcaacagg atatggatac ataaatttta atagagaaaa tactatagaa
     9421 gatgttgcat atgaggtagt ggagtttgta gaaaaaccaa actatgaaat tgctaaagaa
     9481 tatgtaaact ctaaaaagta tgtatggaat agtggaatgt tcgtatggaa ggtatctaaa
     9541 atactagaag attttaagag atacttgcca aaagtatatg aaaaattaga agatataagt
     9601 aaatacttag gaacaaaaga agaaatggaa aaaataaaag aaatataccc aactatacaa
     9661 tcaatctcta tagattatgg tattatggaa agaagtaatg atgttatagt agttcctggt
     9721 gattttggat ggaatgatgt tggttcttgg gattctctag gtgcaatata tccaactgat
     9781 gatgaaggta atatcaaaag aggtgaaaat attactatag atactaaaaa ttcaattata
     9841 tattcagatg acaaattaat atcaacaata ggtattagtg atttgatagt agtatctaca
     9901 aatgatgcag ttatggtttg tagaaaagat aaagctcaag atgtcaaaaa aatagtagag
     9961 cagttaaaag aagaagatag acaagaatat atgtaggagg tgttcatttg attcctaaaa
    10021 aaatacatta tgtatggttt ggtggtccaa aaggaaatat agaaaatata tgtataaatt
    10081 cttggaaaga aaagcttcca gaatatgaga tagtagagtg gaatgaaaaa aactttgata
    10141 tagagaaaga aataaaagga aataagtttt tggaagaatg ttataaaaga aaactttggg
    10201 cattcatatc tgattataca agaataaaag tactctatga acaaggtgga gtttatatgg
    10261 atacagatat gcaaatatta aaggatataa ctcctctttt agaaaataat aggctagtct
    10321 gtgggtatga agatgataga gagtatataa atggtgctat aataggtgta gaaaaaggac
    10381 atcctttttt aaaagactta ttggagtatt atgaaaaaga agtacttact tcttcattgt
    10441 ttacaatacc aaagataatg actcatctta tggaaaagaa ttataagaag attgatccaa
    10501 ataattatga agaaggaata cgtgtttacg acaaagaata tttttatcct tttggattta
    10561 aagaagactt tacaccagaa tgtataacag aaaacacttt tgggatacat tggtggggaa
    10621 aaagttgggc taaaaagaga aattatttct tagaaagcaa acacttaaca ggtgtaaata
    10681 agatatggaa gtgttgtaaa atatttgcaa gtaatacttt acgaagctaa aagggagaaa
    10741 gtaaaatgaa gactttaaat gatgatagaa atattatgat tagaaaatat ctttatatat
    10801 ttgttttgtt tatcatatta cttagaaagt atatgatatc atttattgac ccaaatatgg
    10861 atatagggat gattaaatca gcgttatttt attcttcaat aggaatacta atgctacttt
    10921 ttttatttga taagagaaaa agtataacag aaatggtact agttggagtt tgtgtcttac
    10981 tttatttatt aaatagggaa ggagctatat tattaatcgt tttattagct gtatcagcta
    11041 agcaaataga tgataaattt atagttaaaa attatttaat aatatcggca tgttttttaa
    11101 tggtagctat attattattt aatttatttc cttcattaat atttaatcag gaagtccact
    11161 atagatatat agaaaaaata gatatgcttg ttacaagaat ggattttgga cttggtaatc
    11221 caaatagtgt attttatcat atggttacta tatatgcagc atatatattc ttaagatata
    11281 aagattataa taaatgggat agaatacttt tatttggttc tgcatttttt gtttatcaaa
    11341 ccacatatag tagaactgga tttttcacta tattagcagg attaatattt gtagaaataa
    11401 taagatggat agatataaaa aaaataaaag gactaccaat gctcctaaag acattaccgg
    11461 taatacttac tttatttagt gtaattattg gtacagtatt tgataaaagc actttattaa
    11521 atagattatt agcaagtaga cctaagttct ggaatgtata cttggctgaa aaggggaact
    11581 ttctaaaacc ttttggaaat tcatatagtc ctgctataaa agcaactaat ccattagata
    11641 gttcttatgt atatataatt agtgtactag gagtagttgc ttgtatatta tttatgtatt
    11701 tgatgtataa aggaatagaa agctttatag aaaaagataa aaaagcatat ttagtagctt
    11761 tatttatatt tttactatac tcttttgcag aaaatttatt attagaagct tcatttagtt
    11821 ttgcagtagt attactaata aaagaagtga tattaaatga taaaagggaa atagatttgt
    11881 ggaagatgaa aagtaggagg tagtaatgtt aatatcatta ataatgccaa cattaaatag
    11941 atatgatgat atatacttac ttatggacag cttagaaaat caaacttaca aaaactttga
    12001 acttatagta gtagaccaaa atgataatag caaagttaaa gaaatagttg ataaatacat
    12061 tgataagtta gatataaagt atataaaaag ctctaaaaaa ggattgagtt ataacagaaa
    12121 tgtaggcata gacaatgctg tagggcaaat tataggtttc cctgatgatg attgtgtcta
    12181 cgaaaatgat acattagaaa aagttataaa tttctttaat aaaaataaag attataaaat
    12241 atacagttgc aaaactatgg attcaaataa agttgatgca tttaagaaaa tgtatgatgg
    12301 aacttgtgat atcacaagtt ccaacgtttt ggatactata acatccataa ctttttttat
    12361 tgattttgaa ggtaaagact atacaagatt tgatgaaaaa ttgggtgttg gtggagagtt
    12421 tggtgctggt gaagagatag attatgtttt aaacttattg agtttaggtt ttaaaggaaa
    12481 atactttgga aatgatataa tttaccatcc tgcaaaaaaa cattctaaat ctaaagaaaa
    12541 gtatcaaaaa gattataact atggaagagg ctttggagcg ctttgcaaaa aggaaatagt
    12601 ctatagaaaa aattataagt ttgcaaaagt tatggtatca aaacttgtaa gaaatatagg
    12661 aggtcttata ttgagttcca atagagatta tcatagtgcc actataaaag gaagaattaa
    12721 tggatttaga cagtacaagt tataatgagg atggtgttta tggacaaggt taaattttta
    12781 ataaatatgc ttttggcttt ttttatatat ccatttaata agcataagtt taatggtaga
    12841 gatatctggc ttataggtgg acattcaggg gatatataca atgataattc aaagtttttt
    12901 tatgagtata tgctaaaaga acataatgat gtagaaactt attgggtagt aaataaagac
    12961 agtaaagtat ttgataaaat tccaggcaaa aaattaataa gaggaagtgt agaaaactat
    13021 ctgtattatt acaattcaaa agctatagta ttttcacatg caccatcagc agatatagca
    13081 ccatacaatt ttgctgtacc tgttcttaat tattttcata aaaaaacgat aaaagtgttt
    13141 ttaaatcatg ggactataag ttttaaaaaa agaaaaccta tgaacaaaaa gttcaaaaat
    13201 attatagata acctatataa aagctataat atagttacag caagttctga atttgaaaga
    13261 aatgttatgg taaatgattg gggaatgtta gatgactcag tttatataat tggaaatgca
    13321 agatatgaca atttaccaac aaatgaagtt gcacaaactc gagatatatt atatactcct
    13381 acatggagag attggataaa atttagttca ggaaaattta cggatacaga ttattttaaa
    13441 aatataatga attttttaaa tgatgacaaa ttaaataaaa tattagatga gaaagatata
    13501 aatgtaaaaa tttatatgca tcatcttatg catgagttta tagatgatat aaaagaaaat
    13561 ataacaggaa aacgaattgt gtttttagat aaaggggtaa ctctagcaaa tgaaatcaga
    13621 aaatcagctg caaatataac tgattattca agtgtagcca tagacttttt gtatatgaat
    13681 agacctatct tgttttacca attcgatttg gatgaatata tggaaaaagt tgattcatat
    13741 atagatttaa aaagtgaaat gtttgggtct ttggcttata ataatgatga agccgtgaat
    13801 aaacttattg atattattga aaataatttt gaggttatgg ataaccaaaa aaatgaaaga
    13861 aataagtttt tcagatataa tgataataag aactgcaaga ggatatatga ttgtgtatta
    13921 agtaaaatta aataaatatt ttataaatga tgaggaaggt aatatgaaga agaatttagt
    13981 atctataatt actcccatgt ataattctga aaaatttatt gaagcaacca taaaatcagt
    14041 attaaaccaa acttatcaag aatgggaaat gttaattatt gatgattgct caacagataa
    14101 tagtcctaat atagtcaaat cttatatgca acaggatagt agaataaaat gtataaagac
    14161 tgagactaat aagggtgtct ctaatgctag aaatttagca ctaagtaagg caacaggaca
    14221 atttatagct tttttagata gtgatgacca atggaatagt agtaagttag aaaaacaagt
    14281 aaattttatg ttagaaaatg actatgtaat ttcatttact tcatatgaac tgatggatga
    14341 aaatgataaa aaattaaaca aagtaataaa agttccacct aatgtagatt atagaagatt
    14401 attaaaagga aatatcttag gatgtcttac tgttgtgatt gataagtcta aattggattt
    14461 tgaaataaga atgagtggtg taagacatga agattatgtt ttatggttat cgatattgaa
    14521 aaaagggcat attgctcatg gaataaatga agttctagca ttatatagaa aatctagcaa
    14581 ttccctaagt ggtaataaaa taaaagctgc tatgtggact tggaatatat atagaaatat
    14641 agaaaaaata cctttataca aagctatata ctactttatt aattatggaa taaacggaat
    14701 caaaaaaagt tagacagaga ggaagattta attaatgtat aaatataaat ttactgtatt
    14761 tactccgacg tataacagag ctcatttact tgaaaatttg tacaatgatt taaaagctca
    14821 aacttatgat ttcaatgatt ttgaatggtt gattgttgat gatggaagta gcgacagtac
    14881 taaagagtta gttgaaaaat ttatatcaga aaataaatta aatataagat atatatacaa
    14941 agaaaatgga ggaaaacata ctgcaataaa tctaggtgta aaaaatgcag atggagagct
    15001 atttttcatt gtagatagtg atgatggttt aattaaagat tctcttgaaa ttgctaatga
    15061 tgagtgggat tctttagaga ataaagaagg atttagtgga gttgttggac tttgtgtata
    15121 tccaagtgga gatttgatag ggacagaaat gcctgaagat aagaaaatat gtcactttgc
    15181 agacctatat tttaagtatg gcgttaaggg agataaaagc atagtatttg taactgatga
    15241 gcttataaaa tttccttttc cagaaagaaa agaagtgaga tttttaccag aaagtgtagt
    15301 atggaatgaa atgtctaaat actataaagt tatatgtgta aataaaccta tgataataag
    15361 agattatttg gatgatggtt tgactaagaa tatattaagt aaaaatgcat taagaggtag
    15421 agcattagag tttttatatt taataaatca aaatacatat ccattaagta gatatccata
    15481 tatgtggata aaaaattaca taaatttagc cagatactca ttgttgtctg atagtcatta
    15541 ttttggtgaa ttaagaaaag tatctgataa attgttgtat ttagcattat tcccattagg
    15601 atattacaaa tatattggac aaagaaaatt agtatctaag taaacttgtt ggtatagtac
    15661 tgctagttgc tatataagga gagtaaaaat atgaatgagc ctttagtatc gataattact
    15721 cctgtttata attcagaaga gtttttatca gagacgataa agtcaatcca aaatcaaact
    15781 tataaaaatt ggcagctttt attagtggat gattgttcta aggacaattc aagtgaaatt
    15841 ataaaaagtt ttcgaaaaga agatgctaga ataaaatata taaagcttga aaaaaattct
    15901 ggagctgcag tatctagaaa tgttggtatt aagaatgcgg aaggtcgatt tattgcattt
    15961 gtagatagtg atgatttatg ggatagtaga aaattagaga ttcagataga atatatgtta
    16021 aaagaaaatg taggtttttc ttttacatct tatagatata tgagacaaga tgggtcaaaa
    16081 acgaataagg ttgcaagagc tcctaagaaa attgactatg aaggattact tagaaatact
    16141 ataataggat gttctacagt ggtcattgat aaggagattg taggagagtt cagcatgcct
    16201 ttggtaagaa gaggacaaga tactgcaacc tggttacagt tacttaaaaa ggaaaaatat
    16261 gcatatggga ttcaagaaga tttagtgaat tacagattag ttggaaattc tatatcaagt
    16321 aacaagataa aagcattaaa acgaacttgg aatacttata gaaatgttga aaatttaagt
    16381 ttaccaaaaa gtctatatgt attttgtttc tatgttttta atgccattaa aaaaagagta
    16441 taagttttat atacttggag gtaaaaaaat gaaaatagct atagctggga cgggatatgt
    16501 aggtttggtt acaggtgttt gtctagcaga ggtaggcaat gaaaatgtag tctgtgttga
    16561 tgtagatgaa caaaaagtta aatcaatgaa acttggaata gcacctatat atgaagaagg
    16621 cctagaagaa cttatggtta aaaattacaa cagaggaaga atagattaca ctacagacta
    16681 taaaaaagct tatgaagatg tagatgtaat attaatagct gtaggtacac ctgagagaag
    16741 tgatggttca gccaacctag attatataaa ggcagttgca aagcaaatag ctgaaactgt
    16801 gacaaaagat actctagtag taataaaatc tacagttcca attggtacaa atgaagagat
    16861 agaagattat ataaatagaa acctaaaaaa tgatgtaaaa gtagaacttg catctaatcc
    16921 agagttttta tcacaaggaa cagcagtaaa agatactcta catgcttcaa gaatcgttat
    16981 aggtgtagaa agtgaatggg ctagagaaat attagaggaa atctataagc cttttaatca
    17041 acctataata gttacaaata gaaatagtgc tgagatgata aaatacgcat caaataactt
    17101 cttagctctt aaaatatcat ttatgaatga tattgcaaat ctttgtgaaa tagcaggagc
    17161 taacatagaa gatgtagctt taggaatgag ttatgatgat agaataggaa gtaagttttt
    17221 aaaggcggga ataggatatg gtggttcttg ctttccaaaa gacacaaaag ctcttcactg
    17281 gttatctgaa cataatggtt atgaattaaa aacagtaaaa gctgcaatag aagttaatga
    17341 aagtcaaaaa cttaaattag ttagaaaggc atctaagtta gtagagagct ttcaagatat
    17401 gaaggttgct gttttaggtt tgacttttaa gccagacact gatgatataa gagaagcacc
    17461 atctatacca aatataaaat atttgctcga aagaggggca agagttactg tatacgaccc
    17521 aattggtgta aataatacta agaaaatatt tggtgactct atactttatg ctgattctgt
    17581 agatgatgcg atagaagatg cagagatatg ttttataatg acagaatgga aagatgtatt
    17641 aaattatgat atacataagt ataaggaaaa gatgaaaaat cctttagtcc ttgatggaag
    17701 aaattgttat aaaattaaag atatggaaga gttaggaatt gagtattatt cgataggaag
    17761 gtaaatatta tgaagactgt ttgttattta gctgatgggt caaatcctca tactttaaaa
    17821 tggtgtagtt tttttaaaaa taaaggatat gacattcatg taatctcttt aaatggaggt
    17881 cacatggaag gaataaaaat ttataatttt ggttctaatg tagaagaact aaaaaatgaa
    17941 aatatttcta aaaaaacggg atacttaggt tcaataaagc agataaagaa gctagtaaat
    18001 gaaataaagc cagatatatt gcatgcacag tatgcaagta gttatggatt tataggaagt
    18061 ctacttgggt atcatccata tattatttct gtttggggta ctgatattta tgatttccct
    18121 aataatggtt ttatacaaaa acaaattata aaacataact ttagaaaagc agactatatc
    18181 ttttcaaata gtagagatat ggctaaagag gctaataaat atacagctaa acatgtagat
    18241 gtaacctttt ttggagtaga tatggatagg tttaagccta tggaagtaga aaaagaagac
    18301 gcttttgtaa taggaattat aaagagttta gaaaaaaaat atggaataga atatttaata
    18361 caagcattta agatgcttaa agatgaatat aaagataaga aaataattct taaaataggt
    18421 ggctctggaa gtcagatgga caacctaata aatttagcta aagaattggg tatagaaaat
    18481 gatgttcagt ttttaggaag aatatctcca gaaaatgtaa gcaagacttt taattctttt
    18541 gatgtaactg tatttccttc tttaagagag ggatttggag tggcagctat agaatcagaa
    18601 gcatgtgaag tacctgttat agttacaaat gtaggaggac atccagaatc tgtatgggaa
    18661 aatgaaactg gcttaattgt agaaccaaaa caaccagaag aaataaaaaa tgctataata
    18721 aaactaatgg aaaatgatga acttagactt aatatgggta agaaagggcg tcaatttgtt
    18781 agagaaaatt atgaggttaa tttaaacttt aatgatatag aaaaaattta tgattctata
    18841 tttgataaat ataaaaaata aaatgaaaaa atacagcaaa agatagatgt tgttggctat
    18901 gcttaggtgc gtagcttttg ctgtttaaag gagggcttct agttatgcga gaaagtatag
    18961 atttagagaa agaaagcttt tttaataggc tttttaaaaa atataaattt agacatttaa
    19021 cactattaat tctagatatt tgtagcgtac tagtagcgtt tcgaatgtct ttaagcttga
    19081 caggtaattt aaatgcattt accactaatg atataataat ttcagtgtgt atatatatag
    19141 tacttcatat agtatcattt agattgttta agtgctacaa cacattatgg agatatgcag
    19201 gagaagaaga aattatatct atttttgtgg caactttagc ttatttaatt ccgatttata
    19261 taataaataa attattaggg tttgattatc ctataatgtt ttatgtatta aatacaatat
    19321 ttattataat gtttacaagt ggcgcaagaa tagcctatag agctataaga atagtcatga
    19381 acaagacata ttctagagga aaggtttcaa atatccttat aattggtgca ggagatgcag
    19441 gagaaatggt tatacaggaa ctaaaaagaa atccagaact taaaaaagta gcagtggcta
    19501 taattgatga tgataaaaat aaaattggaa gaataatcca taatgtgaaa attgtaggaa
    19561 caacttcaga tataaaagat gtggtagaaa agtacaatgt agatgagata atcttttcaa
    19621 tagcaaacat agagaaacgt agaaaaaaag atattattga tatatgtaaa aacactaact
    19681 gtaaaataaa gacaatacca ggaatttacg aaattataga tggaaaagtt gatataaaac
    19741 agattagaga agtagagata gaagacttgt tgggtagaga gcctataaaa acaaatttaa
    19801 gagagataag taattacatt gagggaaaag taatcctaat aactggagga ggaggctcaa
    19861 ttggttcaga gctttgtaga cagattgcag gatttaatcc taaagagctt attatagttg
    19921 ataattatga aaataatgct tactcaatac aacaagaatt acttagaaaa tataaaaata
    19981 aattagattt gaagacagta atagcatcaa taagagaaga aaaaagaatg gatgaaatat
    20041 ttaataaata taaaccagaa gtggtatttc atgcagcagc acataaacat gttcctctta
    20101 tggaatcgag ccctggcgaa gctataaaga ataatatatt tggaacttta aacatagctg
    20161 gcttgtcttc aaaatataga gctaagaaat ttgtccttat atctacagat aaggctgtaa
    20221 accctactaa cataatggga gctactaaaa gagctgctga aatgattata cagactatga
    20281 atgctgaaag ccagactgaa tttgttgctg ttagatttgg aaatgtgctt ggtagtaatg
    20341 gtagtgtaat tccacttttt aagaagcaaa ttgaagatgg cggaccagtg acagttactc
    20401 atccagatat tattagatat tttatgacaa taccagaagc agtaggctta gttatacaag
    20461 ctggggctat ggctaaaggt ggagaaatat ttgtgcttga tatgggagaa cctgtaagaa
    20521 tattggattt agcaaaaaac ttaattaaat tcagtggatt tgagccagat gttgatataa
    20581 aaattgaatt ttctggtctt agaccaggag aaaagttgta tgaagaacta cttatgtcag
    20641 aagaaggtct attggataca gagcataaaa agatttttat tggtagacca atagatgttg
    20701 atagagaaaa aataacaaaa tacttaaaac ttttaagaga aattactaac aatgaagagg
    20761 ttgaaaaaat agatggtatt atgagggaat tagtaccaac ttatattaaa ccagaagatg
    20821 ctaatatcaa agaaattgca acaactagag aaaagtagaa tatatttcta atttgtaacc
    20881 agggggagca aaaagaaaca aatatatact aaaaagaaac aaaagattac aaaaagatta
    20941 caaaaagatt acaaaaaagg ttgcaattta ttttgatgta agatagaatg ttaattagga
    21001 attggaaacg aaggaacaaa atgttaaatt ttcgaaagga ttaggtattt gagattatga
    21061 tgaatattaa aaataaaaaa catatattga aaaaatttat agctatggtg ttaatagcag
    21121 gagttgtcac agtagaagca ggagcaataa cggctagtgc agcagagcca actaattcac
    21181 ctatgagtgc aacagtagac cagtgtgatt ttttaaatgt aagaagtgga gctagtgcaa
    21241 atgatgcagt agtaggaaag ataaacacag gagacaaagt tgaagtttta gaattacatt
    21301 caaatggatg gattaaaata aaaagtgttg acaatgttac aggatgggta aatggagatt
    21361 atttaactat tcaaggtgga aatgtagatg caaaggtaca aaatgtttta aacttagcat
    21421 ttaagcaaca aggtaagcca tataaatggg gagcaactgg accaaattct tttgattgtt
    21481 ctgggttcac ttcttatgtc tataaaaatg gagcaggagt taatttacca agagtatcaa
    21541 gatcacaagc aacagtagga aagaaagtat caagagcaga attaaaacca ggagatttag
    21601 ttttctttgg ttcaggtggt tcaataaatc acgttggatt gtatgtagga gattctaaat
    21661 tcatacattc accacaaaca ggagatgtag taaaagttac tagcatggct ccaggaacta
    21721 attatgcaaa aagacttata actgctacac gtgttttaca ataataatag atattgattt
    21781 acataggatt gaatattaac attaaatata taattacatt taatgtttta catagagatt
    21841 taagagttta gtaatattaa aatagtgatg aacgttataa aaacagcaaa gaagataaat
    21901 ttctttgctg ttttttttat aataaattat ttcatattat aatcaaatat caaatataaa
    21961 ttttaactat attattcaat atttgacaga taatatgaat tttagatata cttaataaga
    22021 agtatattgt agaaaaaata aatttaaaaa attaaggggg atgaaatgaa aaaaataagt
    22081 atattagtat tatcattgat aatgacactt actatgtgta gtgtaagttc atttgctgat
    22141 tcatcaaatg ataaggaaat gagagctgct tggatatcta cagtttataa tttagactgg
    22201 cctaagacta agaataatga agccaagcaa aaaaaggaat acacagattt attggacaaa
    22261 ttaaaaagtg taggaataaa tacagcagtg gttcaagtaa gaccaaaatc agatgcttta
    22321 tataaatcaa atataaaccc atggtctgaa tatcttaccg gtacacaagg taaagaccct
    22381 ggatatgacc cattaccatt tttaatagaa gaagcacata aaagaggaat ggaatttcat
    22441 gcatggttta atccatatag aataactatg gcagatgaga gcatagataa attgccagca
    22501 aaccatccag caaagaaaaa tccaagctgg gtagttaaac atggaaataa gtattactac
    22561 gacccaggtt taccagaagt tagaaagtac atagtggata gtatagcaga agtagttcaa
    22621 aattacgata tagatggagt tcattttgat gattacttct atccaggagt aagtttcaat
    22681 gatactgcaa cataccaaaa atatggaaaa ggacaaaata aggatgattg gagaagagaa
    22741 aatgtaaata ctctacttag agatgtaaaa gcgtctataa agagtataaa gcctaatgtt
    22801 gtatttggtg taagcccagc aggtatatgg agaaataaga gtagtgaccc aacaggttca
    22861 gatacatcag gtaatgaaag ttatgtaggt acatatgcag atacaagagc atggataaaa
    22921 caaggattga ttgactatgt agttccacaa ctttactggc caattggtct taaagcagca
    22981 gattattcta aattagtggc atggtgggct aatgaagtaa aaggaacaaa tgtagactta
    23041 tacataggac aaggtatata taagcaaggt caatcgagtt atggtggaca aaatatagca
    23101 aaagaaatag tacaacaagt aacattaaat agaaagtata gtgaaataaa aggtagtatg
    23161 tatttctcag ctaaagatat agcaaatagt acttctatac aaaaggattt aaagagttta
    23221 tattctagta gtgaagaacc agttactcca ccatctaatg ttaaagtgga gaaattaaga
    23281 ggcgacgaaa gatatgacac agcagtagca ataagtaaaa aaggttgggc tacaaattca
    23341 gatacagtag tattagtaaa tgggtactct atagttgatg gtataacatc aacaccactt
    23401 gctacatcaa atgatgctcc tatactattg gtaaataagg ataatatacc tacatccact
    23461 aagaatgagt taaaaagact taatccaagt aaagttattt taattggagg aaataactct
    23521 attggagata aagttgagag tgagataaaa gatacattgt caaatgtatc aataaataga
    23581 gttggtggtt cagatagata tagtacatct ttaatgatag caaaagaatt agtaaaaact
    23641 aatccagtag aaaaactata tataacaagt ggtacaggtg aagcagattc tctttctata
    23701 gcttcaaaag caggagaaga aaagcaacca atagtattag tatcaaaaga taatgtaagt
    23761 gatgaagttt acaattggat atcagattta aaagtaaaag atgcttattt tataggtggt
    23821 aatctaagta tatctgattc agtaataaat aagctggata aggtcattac aaatgatgta
    23881 tctaaaaata gaatagctgg agaaaataga gaagaaacaa atggaaaagt aatacaaaaa
    23941 ttctatccaa atgctgaata ttcatcaatg tttgtaagta aaagtaatca attggtagat
    24001 gcattaacat caggtccttt agcagctaaa ttaaaatcac cagtagttat gcttggaaat
    24061 agtgtaacat ctgctcagaa aactgcttta gagcctaaga aaactactct agtatacgaa
    24121 gctggagatg gtataaatca aaacacatta aacactttct taaacttagt taagtaataa
    24181 aaaagttaat ttaaaataca gagtatttta taaatgtaga ttaaaatgaa aattatcatg
    24241 ataaattaga aaataaaaaa gaactatatg cattttgtaa tcaagtgtac acagcaactt
    24301 gaaaataatt atgcgatata gttctttttt agtaattaat cttatacttt ttaataaact
    24361 ttatgttgaa ttttttattg tttaaactct atgtcattaa atataaaatc gtgtaatatg
    24421 tctaaagtat ctgggtcaaa tctaagaacc catccagctt ttccatatat accacctgta
    24481 ctatgaattt catcatcaat aggaaattca aattgcttaa tatttaagtc gcccattttt
    24541 aaaacttgag ctcctaaaga aagtattgca ttaggcttca tgttagtttt aacatatgga
    24601 agaactgcat ctaataaacc tggatattta gtaacaggta aagtttccgt tttcttgatt
    24661 atagcttgta taatctgtct ctgtctgcca tctctagcaa aggctgtatc gttgtgtctg
    24721 atacgtgcaa aagatagtgc ctgatatcca tttagagtct gctttccaga gtttctaatg
    24781 tattgtatgc tacctttatc atcactttta ttccatttat aagtttcagg aataaattta
    24841 tttagttctc gtatttctcc tttttcaaca gtaacctcaa cgccaccaat agcatcaatt
    24901 atatacataa atgattcgaa attaacacaa gcataatttt gaatatcaat attaaaattc
    24961 tcttcaattg tttgtattaa taaatcagct tgtccataag cataagcatg agtaagtttt
    25021 tgtttaccat gaccagggat gtctacatag ctatcacgag ctaatgatgt aagtttgata
    25081 tcattgtgtt tgttatcaat agtaagtatc atcatagcat ctgaccttga agaatcttca
    25141 tttggtctag catctgttcc cataagtagt atgttaatta tcccatcttc atttttatgg
    25201 tcattgttat ccaataaatc actacttatg ctcgagtcat gaatagcaga taatttataa
    25261 taaaaatatc catagacact tatagggaat attactacca agaaagccag tagtataaca
    25321 aatttcttta attttgacaa aagtacccct tctttcttct ttttcctgta tattttttaa
    25381 tagcatacac ttaattatat taaagtattg agatatattc aatacttgtt tcagttacaa
    25441 aaaaaagaca atgatataat ttataagaaa atttatcgac ctatatatat agttaaaagt
    25501 atatataata tattttttaa aagtaattta tattttaaaa taaaaacaca ccccataaat
    25561 acaaagtaat ttgcatttaa gaggtatgta tttatcatca aattattggt ttgttttgga
    25621 atttctaatc ttcaaccata atatctttaa atatgaaatc atgcattgca tctagttcat
    25681 attcctcaaa aggtattaca tagcctgcat tacctatttt tctaccattt tcagtagaaa
    25741 gaggaaactc catagatttc aaatttaaat ttcctatact taaaagttct tttcctaaag
    25801 atattatttc attaggattc atattagttt ttacatacgg aagtattgtg ttaactagat
    25861 taggatattt agtgactggt aaatccttaa caccatttat taaactctgt ataacacttc
    25921 tttgtcttct atctctttca tctgtactat cattttttct tatacgagaa aatgcaagtg
    25981 tctgatatcc atttaatttt tgctcaccag aatttcttat atatttaaga gaacctttat
    26041 tgtcattttt attccattta taagtttcag gaataaattt atttaattct cttatttcac
    26101 tcttatcaac agtaacagta actccaccta aagcatcaac tatatccata aaagagaaaa
    26161 agtcaactac ggcgtagttt tgtatatcaa gttggaagtt ttcttctata gtttctatta
    26221 ataagttagc ttgtccatat acgtaggcat gtgttaattt ttgttctcca cgtcctggta
    26281 tattaacata agtatctcta tttaatgaag ttagcttaag acttttattt ttattatcaa
    26341 tagtaagtat catcatggca tcagaacgag aaacttcttc tcctggtctt gcatctgtac
    26401 ctaacaataa tatgtttgtg atattttctt cgtttttata gtcattacta ctaagcgcct
    26461 ctgtatttat actatcatct tgtatagttt ttaatttagt aaaaacatat ccatatatac
    26521 taacaggaaa tgctataact aaaagggcta gaaatataac gaactttttg agtcctgaca
    26581 ataatatccc ctactttcta aattttttaa ttacacatag ttaattatat taaaaatttg
    26641 ttacataagc aataaaaaac acaataaatt agcataagtt tgtcataata taattatttt
    26701 tatagcattt tatagaagaa agtgtgtagt tattattgat tttattggaa tataattgta
    26761 tgaagactta aatttttata aactgatgtg tatattgaaa aaaatatttt cctgaatgta
    26821 gttatagcat cttatgaaag gtgcaaattg taatttaaaa tttttagtgt ataataagaa
    26881 ttgaaaattc aaaccgataa aaattaataa aaaataatta taaattaata atttggggga
    26941 ggaaatgtat gataaaatta atagcaacag atttagatgg aactttgtta gatgaaaaga
    27001 gcgaaataaa tccagagttt tataaagtat ttaaaaaatt aagagagaga gggataatgt
    27061 tttctgctgc tagtggcagg cagtatcaaa atcttataaa aaaatttgaa gatataaaag
    27121 atgatatgat gtttatatca gaaaatggaa cactagtagt atataaagga aaagaaatat
    27181 tatcaaatcc tttaaataaa gaattagtaa atgaaatcat agagacaaca agaagtataa
    27241 aagggaaaaa aattgtaatg agtgggaaaa aatacgctta tatagaaagc aaagatgaag
    27301 cgtttataca agaggtcagt acttattatg caaagtttaa aattgtagaa gatttgacga
    27361 aagtagaagg agacatattg aagatagctg tatttgactt caaaggtgca gaacataata
    27421 ataatatcta ctttgaaaag ttttctgata gagctcaagt atgtatttct ggagtggagt
    27481 ggcttgattt aactgcaaaa ggtgcaaata aagggagtgc cataaaaaaa gtacaaaaaa
    27541 tgttagatat aaaatatgaa gaaactatgg tatttggtga ccagttaaat gatgtggaaa
    27601 tgatgaaaag tgcctatcat agttatgcca tggaaaatgc aaatgaacat ttgaaacaaa
    27661 ttgctagatt tagagcaaaa agaaatactg aaaatggcgt tgtagataag ataaaagagg
    27721 ttataaaaat tggatagtca tatgtataga tgaaaaagat tattttgaag aaatcaaaaa
    27781 aatatcaatg taattttgat aaataagttt atttatgcga aaattacatt gatatttaat
    27841 aaacatcaat aatagatatt tattttttta tgatattcag tattttgttt tctatttgag
    27901 ttgcattttc agcagaaatt aaataaacat atctacctgc cttctcaatt atagtattat
    27961 ttgctatttc agaattgatt ttttctccat gtccttctcc aaaaggtgct tgtatcatat
    28021 cttttttata attttctaga gttttataga ttttatctac ctcgtcagtt tttataacga
    28081 taatatcttc tagagataat ttttcagaag aattaattac aaaaccttga attatgtctt
    28141 cctctacatc attaaaatag ttaaaatcct ttgcatcaaa gatttttgat tcctttataa
    28201 gtaaatcaga agattttaat gcttttttta tttttgatgt agaaacgttt ttgtaatcac
    28261 cacttgaaga acaactaacc attcctaatg ataagattat cgaaaaaaac aatattaaaa
    28321 tttttttcat gataaacctc caagtttttt aaactctgtc ttaaattagt ttactacata
    28381 attatataaa aataaatgta tttaatgtta cagttttgat taataatgta atattttgat
    28441 aataatacct attgagaggt gattagaaat gagaatacca aaacatgtag gaattatacc
    28501 ggatggaaat agaaggtggg caagtcaaaa tggtatgaca aaagataaag gatatgaaag
    28561 tggaatagac cctggactag agtcttatag attatgtaga gatttaggct gtgaagaggt
    28621 gactttttat ggatttacaa ctgataatac aagacgtcca tcagtgcaga caaaagcttt
    28681 tacaaaagca tgtattgatg ctgcaaatat attaatagat gaaggtgctg ctttattagt
    28741 tgtaggaaat acaaaatctc caatgtttcc agaagaatta ttaccatata ctaatagaat
    28801 taaagccaaa gaaggtcaaa taaaagcaaa tcttctggta aattatggtt ggtattggga
    28861 cttaaataat atatcaggag tagaaaatgt aaataaaaat aatatacagg attacataca
    28921 gtcatttgat gtatccagac tagacttgat aatcagatgg ggtggaagaa ggagattaag
    28981 tggtttcctt cctgtacaat caatttatgc agatttttat gttatagata attattggcc
    29041 agattttaag agtgacgata taaaggacgc atttagttgg tatgatacac aagatgtcac
    29101 tttaggtgga taattaaaaa tattctgatt taaggttgta gagtgtttta aattattgaa
    29161 aaaaaaactt cttgatggta aaataacact aataatatgt aaaagtgtaa aaatttatac
    29221 caagaagggt gaagaaatgt caaaaggcaa taataacaat aattcaagaa acaaatctaa
    29281 aaagaccagt catctgaata gaaaaaagcg aaaattaaat aagaaaaagt tagcagtgtt
    29341 gatttgcttt acagttttat ttttatttat tgcatttaag gctactcaag gtgtagtagc
    29401 tttggtaaaa agtatggata aaagtaataa aactaatcaa caacaaaatg ttaactcaga
    29461 acaatttgac cttggtaatg aagaggaaaa taaaaagaaa aaatatactg tatttataga
    29521 cccaggtcat ggaggcaatg ataaaggaac tgaaagcaag accagtaata gatatgaaaa
    29581 agacctaaat ctacaaattg caaaaaaatt agcaaataaa ttatctaaac aaaaggatat
    29641 tcaagtagta gtatccagaa ctgatgatac atatatatca ttaaaggata gagctatctt
    29701 agcaaataat agttctgctg atgtgcttgt ttctattcat ttaaatgcag agaaaaatgg
    29761 taatacagca acaggtatag aaacttggta taggaataaa gctacagatg gttcaaaaga
    29821 acttgcacag acagtacaaa gtactatagt atcctatgtt aaagttagag ataggggaat
    29881 agttgaaaat aactttgaag tacttagaga atcaaatatg cctgctatat taatagagtg
    29941 tggtttttta acaacacctt cagaggaaca aaaaataata aatgaaaaat atcaagacca
    30001 acttgcggaa ggaattgttc aaggtgtact ttcataccta gattctaaag ggaataagta
    30061 agttaacttt actttaatgt gaggataaat ttaaaagttt aactgtgtaa attgtattaa
    30121 ctttatttcg taatgttgca aacattgttt gaatttagaa actatgaaat tagaaattat
    30181 tttatctata tctaatttca tagttttttt tgcaaaaaat ttatagttat aaaattttaa
    30241 aaaataaatt tagtataatg taatgtagtt atttatatgt gattttaact tgcattgatt
    30301 tgctatcaat tgtgctttat aattagtata tataaagttt aactgtaaga taatttaaaa
    30361 aataggggaa tttatatgaa taaaaatagc atgacaagaa tttacagttt aaataaaata
    30421 atttttatta taattggaat tttaggaata gttttactca caagacatat tgaggtagat
    30481 attaaagttt taattttaat tttattaacc ttgtttagta taaatgtatt tatttcatat
    30541 cttaaaatta agttgtatga ggataaaatt aatttaggat tgataaataa ttcagataag
    30601 tttataaata ttactaaaga tgagaacttc ctaaaacatg ttcaaaatta tattatcagt
    30661 aatgaaaaag aaaactgtgt aatggcttgt tttgatttat gtagactcaa acttataaat
    30721 gatatatatg gttatgagct aggggatgaa gtgttaaata atattttaag taacttaaaa
    30781 aattattttg gaaaagaagc tatttatgga aagttaaaaa gtgatgtttt tgtattaata
    30841 ataaagcttg aaaataggga acagaagatt ccttatttag taaatcttat taagaataaa
    30901 attgtgatac taagtcagat tgacagtttt aaaatggata taaatattga ggcttctatt
    30961 ggaatttata aatttaacaa taatgaaatt gatataaaaa aagctataga taatgctgat
    31021 atggcaagac ttaaatcaaa aggactaaag cacatagagt atgtagaatt tgataatgct
    31081 atggaagaag aaatacaatc aattatgaaa atagagagag atttattctt ggctattaaa
    31141 aataaagagt ttgtaattca ctatcaacct aaagttgatt caagtacagg taacattata
    31201 ggttcagagg cattgataag atggttgcac ccatcattag gcatggttgg accaaataag
    31261 tttataccta tagctgaaaa aaatggtctg ataaacaata ttggtagatg gctaattcaa
    31321 gaagttttta ttaccattaa caagtggatt tcagaaggta taaatgtctt accagtctca
    31381 ataaatcttt ctagagtaga actatataaa aatgatttag ttgatttttt taaactgatg
    31441 tttaatacat ataatattcc aaaagaactt atagaattag aaattacaga aacgacagca
    31501 ctaagagatg taaaatttat aagtgaaagg ctttatgaga ttaaatcttt aggtatgaat
    31561 atatcattag atgattttgg agtaggtaat tcaaatttta tcaaccttaa ggggatacca
    31621 ctagatatta taaaaattga taggtcactt atattggata tagttacaaa tacaaaaaca
    31681 aagtttatgg tgaaagcaat tgttaacctt tcacatgatt taaacgtaac tgttatatgt
    31741 gaaggggtag aggatatgca tcaggtcaaa gtcttaagtg aactgggatg taatttcata
    31801 caaggttatg tattttttaa acctttggat gagatgaatt ataaaaaact tcttactgac
    31861 ggaagttata taaatctgaa agatactctt ttagataaat gcatgacagt tgaggaggag
    31921 taaggatgat ttcagaagat gtagattatt caataagaat cataagagct atatttaata
    31981 atggagaaga taaaccaata gatgctaaaa tgatattaaa aagtgaagat ataccaatac
    32041 taacactata cagactgctt agaaagcttt gtgcaaacaa tattcttact tttaacaaag
    32101 aagatggcaa gacatacatg ttaaatgtgt cattggatac aataagtcta agaactattg
    32161 tagaagtaat tgatggaaaa atatatataa ataaatgtac gagatatgta tctaattgca
    32221 gtaattgctt aaaaggttgt aaagtaaatc gtgaattgct agaaatagaa aaagaaattt
    32281 ataaactttt atcgagaaaa tctataagac aaatactaga ataaaaaatt ttcagctatg
    32341 ataataattg gaggttttcg gttatggatg atatatctca agacaatttt ctactgtcta
    32401 aagagtatga gaatagctta gatgtagata ctaaaaaagc aagtggaata tattatactc
    32461 ctaaaataat tgtagattat attgtaaaaa aaactttaaa aaatcacgat ataattaaaa
    32521 atccatatcc acgaatactt gatatttcct gtgggtgtgg aaattttctt ttagaagtat
    32581 atgatatttt atatgactta tttgaggaaa atatatatga attgaaaaag aagtatgatg
    32641 aaaattattg gactgttgat aacatacacc gtcatatatt aaactattgt atatatggtg
    32701 ctgatattga tgaaaaagct ataagtatat taaaagatag cttaacaaat aaaaaggtag
    32761 taaatgattt agatgaatct gatataaaga ttaatctatt ttgttgtgat agtcttaaaa
    32821 agaaatggcg atataagttt gattacatag tgggtaatcc accctatatt ggtcataaaa
    32881 aacttgaaaa gaaatataaa aaatttctcc tagaaaaata tagtgaagtt tataaggata
    32941 aagctgactt atatttttgt ttttataaaa agattataga tattttaaag caaggaggga
    33001 ttggcagtgt tataactcca agatattttt tagaaagtct atcaggtaaa gatttaagag
    33061 aatatattaa atcaaatgtc aatgttcaag aaatagtgga ttttttaggt gcaaatatat
    33121 ttaaaaatat aggggtatct agttgtattc taacttttga taagaaaaaa acaaaagaga
    33181 catatataga tgtatttaaa atcaaaaatg aggatatatg tataaataaa tttgaaacat
    33241 tggaagaact tctaaagagt agcaaatttg aacattttaa tataaatcag agattgctat
    33301 ctgacgaatg gatacttgta aataaagatg atgaaacttt ttataataag atacaagaaa
    33361 aatgtaaata tagcttggaa gatatagcaa taagttttca aggaataatt acagggtgtg
    33421 ataaagcgtt tattttaagt aaagatgatg taaagttgaa ccttgtagat gataaatttt
    33481 taaaatgttg gataaaaagt aagaatataa ataaatatat agtagataaa agtgaatata
    33541 ggctaatata ctcaaatgat atagataatg agaatacaaa taaaaggata ctagatgaaa
    33601 ttatagggct atataaaaca aaattagaaa acagaagaga atgtaaaagt ggaataagga
    33661 aatggtatga acttcaatgg ggtagagaaa agcttttctt tgaaagaaaa aagataatgt
    33721 atccttataa atcaaatgaa aatagatttg ctatagatta tgataataat ttttcaagtg
    33781 cagatgtata ttcttttttt ataaaagaag agtatttaga taaattttca tatgaatatc
    33841 tagttggaat tttaaattcc agtgtttatg ataaatactt taaaataact gctaaaaaaa
    33901 tgagtaaaaa tatatatgat tactatccta ataaggttat gaagataagg atttttagag
    33961 ataataatta tgaagagata gagaatttat caaagcaaat aatatcaatt ttattaaata
    34021 agtctataga taaaggaaaa gtagaaaaat tgcagataaa aatggataac ttgataatgg
    34081 attctttagg tatataagca gttgtaagta ttaaaaaatt ataatctttt aattgtttat
    34141 ttaaatttag aaataaaaag ttagccatat aaaaaatatt aaaaatatat acttttcaat
    34201 atttttttga taaaataaga atgaatttgt gcaattttat ttttctccaa taactaaaaa
    34261 tagttttttg aattaatatt tgcttaagaa taaaaatcag aaacgttttt gatatagtag
    34321 gtgataatat gaaaggatta gttttagaag gtggaggaac aaaaggagcc taccaaatag
    34381 gtgcatataa agctttaaga gagcttggta tagagtttca aggagtaact ggcacatcaa
    34441 ttggtgcttt aaatggagca tatatagttc aaaatgatat tgaagtaatg gaagatattt
    34501 ggttaaatta tgactatact cattttatga atattgatag agaaacctat gagaggttta
    34561 aaaatgtaga tttcactact aaaaatctca atgcagtgtt tgagcttata aataaagcta
    34621 gaaaaaatga aggtatagat ataagcccat taagacaact aatggagaca acattaaatg
    34681 aagacataat aaggaaatca aaaaaagact ttggacttgt tacggtctgg tgggataaaa
    34741 aaattaatcc atatccattg tatatcgaag atatacctaa aggaagattg attgattatt
    34801 taattgctag ttccagtttg cctatttttc aaacgaacat gatggatgat aagttgtatc
    34861 ttgatggaat gttttgtgat aacatgccaa taggactctt ggaagacaaa ggttatgaag
    34921 atgttgttgt aattaggttg attgatgatt ttattgggaa aatgaatcta aatcgacatc
    34981 aaaatataaa tataaaaact ataataccat cagaatatct aggaggttct cttaataaag
    35041 atagagataa tgtagaaaaa aacatccgac taggttactt agatactatg aaagcatatg
    35101 aaagatgcac tggaattaag tattattttg atgtagagta taaatataat gaagaatatt
    35161 gttttgataa atttagaagt atgagtaatg aaacaataca atatatctgt aatttattga
    35221 atataaaaag agatgcttca cttagaaccg ttatggaaag tgtaatacca aagcttggtg
    35281 aggtgcttgg attgccaaag gatttttcat ataaagattt gttttactgt atatatgaaa
    35341 agaaacttga agaaaataat ataaacagga taaaattata tgattttaac aaggttatag
    35401 atgttgtaaa tacaaatatt gattctaata gaaatttagc ttctaatttt gaagttagac
    35461 ctaaaattag tattaaaaag aacaaaaaca caaaattatt gacaaattgt attataaaag
    35521 acataaaaaa tcaaagttaa ataaaaaaca aaaaaaaatt tgtaattata ctcgaaaaaa
    35581 gtgtatatta gtagtataat atttttgtag cactagtggg aacaaaaaat gaccatagga
    35641 caaaaaagta ccacttagta ccattaaagt aaaaaacagg aaaacaattt taaaaaatat
    35701 aaaatgtaaa gtttatagga ggatttaaaa aaatggaaaa agtagttagt atcaaaaatg
    35761 caagtggatt acatgctaga ccagcaggaa tgtttgttaa gaaggcagct gaatttaaat
    35821 caacagtaga ggttgtagca aaagacaaaa ctgtaaatgc aaaatcaata atgggaataa
    35881 tgagtttagg tttagctcaa ggagaagaat taaagttagt tgtaaatgga gaagatgaag
    35941 aagtagcttt agctgcaatg gttgaattag ttgaaagtgg atttggagaa tagatataat
    36001 aattttttaa aaatttgata tttagaatct aatattactt aaagtaaagt aacagagaag
    36061 tattaaacat atacagacca acatttgtag atatttaatt tctttgttac tttcatgttt
    36121 atttaatcag aagattaaga ctattgttaa ttgggtataa aaatataaat attttagaaa
    36181 atgaaataag atatttttaa tcataaattc agagtaaaaa tacagtaaca aatagtttat
    36241 aaaattttat aaaaaattat aagtaaataa aactataaaa atgtaaatta tttatataaa
    36301 tttataaagt ttatgtgcta gatgatattt tttaggaggt agggttatgg cttacaaagg
    36361 aataggtgct tctcctggtg ttgctttagg gaaagcgtta gtagttgaac atagtgaatt
    36421 agtaattgaa aaaaaatcta tagataatgt tgaagctgaa attgctaaat tagaagatgc
    36481 agttgcagtt tctaaagaag aattagtaaa agttaaggaa aaggcttctg aagaattagg
    36541 agcagaggaa gcagaaatat ttgaagcaca tttattagtt ttagaagacc cagagttaat
    36601 tggttctgca atagataaaa ttaaaactga aagtgtaaat gcagagtatg ctttaaatga
    36661 aataaaagag atgtttgtta gcatgtttga gtctatggac aatgagtaca tgaaagaaag
    36721 agcggcagat ataaaagatg ttacaaatag aatacttaga catatattag gaattaaagt
    36781 tgttgattta tctgctttaa gcgaagaagt tgtattaata gcacatgatt taactccatc
    36841 agacacagca actatgaata aaaaaatggt tttaggattt ttaactgata taggtggaag
    36901 aacttctcat actgctatca tgtctagaac tttagaaata gctgcaatcg ttggattaaa
    36961 tgatataact tcaaaagtta aggatggaga ttttgtggtt ttcaatggag acacaggtga
    37021 agtaatcgtg aatcctgatg aagagactat aaataaatat actgaattaa aagctaagta
    37081 tgaagatgag agaaaagcat tacagctatt aaaaggaaaa ccatctgtaa ctttagatgg
    37141 aaaacatgtg gaattagctg gtaacatagg aacacctaac gatatagaag gtctaataaa
    37201 gaatgatgct gaaggtgttg gtctttatag aacagaattt ttatacatgg atagagattc
    37261 tttcccaaca gaagaaatac aatatgaagc atacaaagct gtattagaag gaatggatgg
    37321 aaagcctata gttataagaa cattggatat aggtggagat aaagaattaa gttatttaag
    37381 tatggaacct gaaatgaatc cattcttagg ttacagagca ataagacttt gcctagatag
    37441 aaaagatata tttaagactc aattgagagc tttatataga gctagtgttc atggaagatt
    37501 aagaatcatg ttcccaatga tttcatcttt agaagagttg ttacaagcta aagaagttgt
    37561 aaaagaagtt ttagcagaac ttgattctga aggtgtggct tatgctaaag atgtagaaat
    37621 tggtatgatg atagaagttc catctgctgc tgtgatatct gatgtattgg ctaaacacgt
    37681 agatttcttc tctataggaa ctaatgattt aattcaatac acttgtgcag ttgatagaat
    37741 gaatcaaaag ataagttatc tttacaatca atttaatcca gctgttttaa gacttataaa
    37801 gactgttata gacaatgcac ataaagaagg aaaatgggca ggtatgtgtg gagaatctgc
    37861 tggagaccaa aaaatgatac caatactttt aggtatggga ttagacgagt tctctatgtc
    37921 tccaatatca atacttcctg ctagaaagtt aataacttca gttaaagagt cagatatgaa
    37981 aaaattagct gatgatgtat ttaatatggg tactgctgaa gaaataaaaa gctatataga
    38041 aaagactttt aatatataat taatgttata tagttactaa aattcatttt tctaaataac
    38101 aaaattatat tttacgaata
//
LOCUS       DAEOSP010000016        36932 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.016, whole genome shotgun sequence.
ACCESSION   DAEOSP010000016 DAEOSP010000000
VERSION     DAEOSP010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36932
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.016"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            802..1824
                     /locus_tag="KRH54_000632"
     CDS             802..1824
                     /locus_tag="KRH54_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG8578305.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            2120..3040
                     /gene="pyrB"
                     /locus_tag="KRH54_000633"
     CDS             2120..3040
                     /gene="pyrB"
                     /locus_tag="KRH54_000633"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBG8578306.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            3079..3771
                     /locus_tag="KRH54_000634"
     CDS             3079..3771
                     /locus_tag="KRH54_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBG8578307.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            3764..4666
                     /locus_tag="KRH54_000635"
     CDS             3764..4666
                     /locus_tag="KRH54_000635"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG8578308.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            4751..5335
                     /locus_tag="KRH54_000636"
     CDS             4751..5335
                     /locus_tag="KRH54_000636"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBG8578309.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            5550..6476
                     /locus_tag="KRH54_000637"
     CDS             5550..6476
                     /locus_tag="KRH54_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HBG8578310.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKNISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(6528..7418)
                     /locus_tag="KRH54_000638"
     CDS             complement(6528..7418)
                     /locus_tag="KRH54_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG8578311.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            7606..8637
                     /locus_tag="KRH54_000639"
     CDS             7606..8637
                     /locus_tag="KRH54_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBG8578312.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPQEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
     gene            8931..9806
                     /locus_tag="KRH54_000640"
     CDS             8931..9806
                     /locus_tag="KRH54_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HBG8578313.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            9824..11314
                     /gene="cls"
                     /locus_tag="KRH54_000641"
     CDS             9824..11314
                     /gene="cls"
                     /locus_tag="KRH54_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG8578314.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSYCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            11507..11791
                     /locus_tag="KRH54_000642"
     CDS             11507..11791
                     /locus_tag="KRH54_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBG8578315.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            11824..13452
                     /gene="groL"
                     /locus_tag="KRH54_000643"
     CDS             11824..13452
                     /gene="groL"
                     /locus_tag="KRH54_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBG8578316.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            13714..14454
                     /gene="larB"
                     /locus_tag="KRH54_000644"
     CDS             13714..14454
                     /gene="larB"
                     /locus_tag="KRH54_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBG8578317.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            14538..15272
                     /gene="larC"
                     /locus_tag="KRH54_000645"
     CDS             14538..15272
                     /gene="larC"
                     /locus_tag="KRH54_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBG8578318.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            15302..15721
                     /locus_tag="KRH54_000646"
     CDS             15302..15721
                     /locus_tag="KRH54_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBG8578319.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKLSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      15781..15882
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            16103..17638
                     /gene="guaA"
                     /locus_tag="KRH54_000647"
     CDS             16103..17638
                     /gene="guaA"
                     /locus_tag="KRH54_000647"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBG8578320.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            18177..19607
                     /locus_tag="KRH54_000648"
     CDS             18177..19607
                     /locus_tag="KRH54_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG8578321.1"
                     /translation="MILNQIFIEAGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDID
                     NNTERYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVSIMEKEKPDIIVAVAHTGEKPKKPRNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(19755..20105)
                     /locus_tag="KRH54_000649"
                     /pseudo
     CDS             complement(19755..20105)
                     /locus_tag="KRH54_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            20100..20621
                     /locus_tag="KRH54_000650"
     CDS             20100..20621
                     /locus_tag="KRH54_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG8578322.1"
                     /translation="MFSIGGGYAIIPLIQEKVVNQTGWISEKMFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAFAATLGCVLCGVVIALFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIISGILGIFFYL"
     gene            complement(20750..21544)
                     /locus_tag="KRH54_000651"
     CDS             complement(20750..21544)
                     /locus_tag="KRH54_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8578323.1"
                     /translation="MIFCNSFITRKSSMNIVVTHTPIVTTLEMQKAYEPLAKHNVNIF
                     AFDFSGTGKSDGYEKDFSRNSIVKDLDSVITYIEKNYSSNIHLYGNTGIGGMFAQYYA
                     CSDNKLKSFAQFACVDYKNTTGVGYTYLTVKLLNFFFKFLPNFHITVKPPKYKGYNEV
                     YDNEFYKIFEKKNPHIWKNSTKIINTMLECFIAEDSKIKDGVNIPTLVFKTLHDRYFK
                     TQYFDRYYNSLKCKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(21768..25196)
                     /gene="uvrA"
                     /locus_tag="KRH54_000652"
     CDS             complement(21768..25196)
                     /gene="uvrA"
                     /locus_tag="KRH54_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG8578324.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTSPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKTGSQNKTIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            25515..27749
                     /locus_tag="KRH54_000653"
     CDS             25515..27749
                     /locus_tag="KRH54_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG8578325.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTASYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGEGTIEIINSKGKPLFIGKNTELIKDLSHKYKDSTDEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFSSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            28005..30035
                     /locus_tag="KRH54_000654"
     CDS             28005..30035
                     /locus_tag="KRH54_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8578326.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKFSSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            30032..30484
                     /locus_tag="KRH54_000655"
     CDS             30032..30484
                     /locus_tag="KRH54_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8578327.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            30486..31541
                     /locus_tag="KRH54_000656"
     CDS             30486..31541
                     /locus_tag="KRH54_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBG8578328.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLIGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     IGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            31608..31916
                     /locus_tag="KRH54_000657"
     CDS             31608..31916
                     /locus_tag="KRH54_000657"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBG8578329.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            32020..33285
                     /locus_tag="KRH54_000658"
     CDS             32020..33285
                     /locus_tag="KRH54_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBG8578330.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGIFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            33373..34089
                     /locus_tag="KRH54_000659"
     CDS             33373..34089
                     /locus_tag="KRH54_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG8578331.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGYEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(34284..34577)
                     /locus_tag="KRH54_000660"
     CDS             complement(34284..34577)
                     /locus_tag="KRH54_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBG8578332.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(34583..34798)
                     /locus_tag="KRH54_000661"
     CDS             complement(34583..34798)
                     /locus_tag="KRH54_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578333.1"
                     /translation="MRILSETEKISLAAIIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            35246..35644
                     /gene="tnpA"
                     /locus_tag="KRH54_000662"
     CDS             35246..35644
                     /gene="tnpA"
                     /locus_tag="KRH54_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBG8578334.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            35641..36804
                     /locus_tag="KRH54_000663"
     CDS             35641..36804
                     /locus_tag="KRH54_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG8578335.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNEGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
BASE COUNT        14179 a         4067 c         6448 g        12238 t
ORIGIN      
        1 gatagtacac aaggatatac ttataaagaa cttaaataag tgaaataaat tattaattat
       61 aaggtttata attttaaaaa attagataaa ttcaggtgta aaatactata aaacacaatt
      121 cggaaaagtg tatgttgaac ttcatttaaa atggaatttc aaagttgcta tagtaagtag
      181 tttaagcatt cttcttatat taatctttga gaataatgta tttaaaaaag ttagtaaaaa
      241 atttaactat tgtatattaa tgatagttgc tattgatata ttatctaaaa aaattaagta
      301 tgaaaagatt actgctagga caatcttttc atacttaatt tttaatattt atattatcca
      361 aaattttcat ctttttaact tagaatttga gaatcactat taggaaatag gctttgattt
      421 atgtagggtt aaaaatttca cagcatatct aactttatac taaatccatc aaaaaatata
      481 cgccctataa aagcgcataa aaaaacacat aaaacaatat ttatgcgcac tttaacctcg
      541 aatggtacaa aattctttac ttttacagac ccatattata taattaagta aataatgtat
      601 atttaggtga catattggat aagctaattt atttatttta ttaatataat gatactataa
      661 aagttatata aagtggttta caaaaaagtt acagatagag cttttattaa tgtattgtgt
      721 attagtattt tatccttagg tacacttcac ctacaaaata gaatcataag ataattatgg
      781 gaaaatttta ggaggtttat cgtgaagaaa aagatattaa taccagtatt tgcatcagtt
      841 atggcattat cagtaagttc aattgtaaat gcagatgaag taaatgattc atctcaaaat
      901 aaggatgata aaacaaatac tgaattaaat ttaggtgaat ataaagaagt aaaatataaa
      961 gttgcaaaaa taaaagatgg agtagctata aagataagag aagaaggtca agttcaaaac
     1021 atagcttatt ctggagatga atttacggtt ttaggaactc aaggagaatg ggttaaggtt
     1081 aaagttgaag atggcgaagg ttggttagct actagatatg tagatatatc tgaaggtgta
     1141 ggatatacta acgctgataa agtaaactta agaaaagata agagtgaaag ttcagaagta
     1201 attgaagagc tggaaaaagg aagttcttta ttggttttgg aagataatgg agattggtta
     1261 aaagttaaag atggggaaac agaaggatat gtaaaatcat cttatatatc tgataaagct
     1321 cctgtaatag aagagccaca agtaaaccca agtattgacc aaaatgtaga ttctacaaat
     1381 aataatcaac aaaacaatgc aaatactaat caacaaaata ataattcaaa tgtgccaaca
     1441 gctaactcta atgcagttca agctgtactt aacttggcat attctaagca aggttgtcca
     1501 tatgtttggg gagcagaagg accaaacact tttgactgtt ctggatttac acaatatgtg
     1561 tatagaaatg cagtaggaaa gaatatacca agaacatcta aagcacaatc taaatatgga
     1621 caaactgtaa gtaaagcaaa tttacaacca ggagacttag tattcttcac aactaatggt
     1681 tctggttctg ttagtcatgt tggaatatat gtaggtgggg gtaatatgat acattcacca
     1741 tcaacaggaa aaacagttag tgtaactagt ataaattcat catactatac agcaagattt
     1801 gttacagcaa aaagaatatt ataatcggtt tgtaatatga caaattgata aatacataaa
     1861 tatatataaa cgctctatat tatatttaat ttaatcatgg aaacaaaaaa tgattatatt
     1921 ttaaatgtac tatagagcgt ttttgtatca ctttaaatct aatcaagatt caagttaata
     1981 tgactgttaa ataggagcct tttcatatga tgattatttg tgcaatccct tgacaggata
     2041 gggcaaaggt ggtaagctac taacagtaca aaaatacttt taattttagt tcagagaggc
     2101 taaaaaagga ggacgaaaga tgttaaaatc aagaaattta atccaaccag aagacttttc
     2161 aatagaagaa atagatgaaa tattagaatt agctcaaaaa ataattgaca atccgtcaaa
     2221 atattctcgt atatgtgaag gaaaactatt agctacatta ttttatgaac catcaacaag
     2281 aacaagatta agctttgaat ctgctatgaa cagattagga ggacgtgtag taggcttttc
     2341 agagccaaat tcatcctctg catcaaaagg tgaaactcta ggagatacaa tgaggatagt
     2401 atctggttat gttgatataa tagctatgag acatcctcaa tcaggtgcag cttcagaagc
     2461 agctagatat acggaagtgc catttataaa tgcaggagac ggcaagaacc agcatccaac
     2521 tcaaacactt acagacttac ttactataaa atcattaaaa ggaactttag aaagccatac
     2581 aataggatta tgtggtgatt taaaatatgg aagaactgtg cattctttag ttaaggctat
     2641 ggcaagatat aaaaatacaa agtttgtatt tatagcgcct gaagagttaa aaatgccaga
     2701 ttatataaaa gaagctataa agggacatgc atattatgaa actaataatt tggatgatgt
     2761 tataggaagc ttggatgttt tatatatgac tagagttcaa caagaaagat ttgaagataa
     2821 atcagagtat gaaagactta aaaactatta tattttaaat aaagctaaat tagaaaaggc
     2881 atctaaagat atgttagtaa tgcatccatt accaagagta aatgaaatag acatagatgt
     2941 agatagtgat gatagagctg tatattttaa acaagctaaa tatggaatgt atgttagaat
     3001 ggctttaata ataaaacttt taggtataaa tgaagactaa tagttatcat ataatagtaa
     3061 aacaaaggag attttgggat gtataaaatt ctggaaaata tatatatagg cgaagatatg
     3121 tatagaatga aagtaaaagg taattttgaa ggtaaaatgg gacaattcta tatgttaaga
     3181 gcatgggata cttatccagt actttcaaga cctataagta tacatgatat agatgaagaa
     3241 gggataactt ttctttataa agtagtagga gaaggaactc aaattttgag taacctaaag
     3301 gtaaatgata atataaaatt ggaaggccct tatggtaatg gatatgctaa ggttgatgga
     3361 aaggtagctc tagtaggagg aggaatcggt gtagctccac tatatttggt tgctaaaaat
     3421 ataaaaaatt gtgatgcata tcttgggttt agagaagatg ttatattaga agatgaatat
     3481 aaacaagtat gtaataaagt ctatacaaca gtaggaaata cttttgtaac agatataatt
     3541 gatgttgaga aatatgatta tatattaaca tgtggtccaa ctccaatgat ggaaaaatta
     3601 gttaagatgg tagaaggtac taaaacgaga attatggtat ctcttgaaaa ccatatggct
     3661 tgtggagtag gtgcctgttt agtatgtacg tgtaaaacta atggaggaaa taaaaagact
     3721 tgtaaagatg gtccagtgtt ctggggagag gacgtgattt ttaatgggta atttaagtgt
     3781 gaaatttgga agtgtagaat ttaaaaatcc agtaataatg gcgtctggaa cttttggatt
     3841 tggaaaagaa tataatgaga tttatgatat acaaaaactt ggtggtataa gtagcaaggg
     3901 tcttacttta aataaaaaac ctggtaacaa tggtatgaga gttcatgaga caccatccgg
     3961 tatgatgaat agtgttgggc ttgaaaaccc tggagttcaa ggatttatag actatgaatt
     4021 accttttttt agtaaactag acttagtcag aatagctaat gttggaggtg ggacattaga
     4081 agactactta ttaggggttc aaatgttaaa tgacaagcct atagacatta ttgaattaaa
     4141 tatatcttgc ccaaatgtca aagctggtgg aatggctttt ggtataaaaa atgaagtggc
     4201 aagagaagtt gttcgtgagg taagaaacat aacgaaactg cctttagtaa taaaattatc
     4261 acccaatgca gaagatattg taggaatggc aaaagtttgt gaagaagaag gtgctgatgg
     4321 agtttcatta gttaatactt ttaaagcaat ggcaattgat ataaaaaata gaagaccagt
     4381 atttgaaaat gtatatgctg ggttatctgg accagctata aaacctatag ctttgagaat
     4441 ggtacatgaa gtgtgtaaaa atgtaaatat acctgtaatg ggaatgggcg gaataactaa
     4501 agccacagat gctatagagt ttataatggc aggagctact tgtatccaag ttggaactgc
     4561 aaattttatg aatcctagaa taggtattga aataatagat ggaattaatg aatttatgga
     4621 tagagaagga ataaaaagtt tagacgaaat aagaggaatt atataaggta gaataaatag
     4681 tattttaaag ataaaaaata tagattaaat aaacaataat tttataacat taacatggag
     4741 gaaaaataat atgagtaaag tagatgtagt ggatatatta aagaaaagtg atgcattgtt
     4801 ggagggacat tttttattat cttcaggaaa acacagtaat agatacgtac aatgtgcaaa
     4861 agtattaaga tttccagaat atgctgctga ggtattaagt acagttgttg aacaaataaa
     4921 agacttagat atagacttag tagtaggacc agctatgggt ggagtaatag tttcttatga
     4981 gttaggaaga caattaggaa aagaagctgt atttactgag agaaaagaca atacaatgga
     5041 gttaagaaga ggatttgaag ttaaaaaagg agcaaagata ataattgctg aagacgttgt
     5101 aactactggt aaatcaacta tggagacaaa aagagtatta gaagccttag gtggagaagt
     5161 tgtaggtgtt gcatgtatag cagatagaac taatcatgat ataggtatgc ctatatatag
     5221 tgctataaaa cttgatattc aagtttatga atctgatgag tgtcctttat gtaaggaagg
     5281 aaaattacca gttgttaagc ctggaagtag ggagttcaag gaattaggga tgtaataaaa
     5341 acttaattat ttatagtgtt acttaaaaaa tgtaaatttt ttagagtaaa aatttgtaat
     5401 ataacatata acttaaaaat aaaaagcagt tagccttata aatgaaggtt agctgttttt
     5461 tttgttaaaa atatatttac aaaataagag atagtagcag tacaatataa gtataaggta
     5521 aaagtattat atattaggga ggggtaagta tgaaaaatca attaaatgaa aatcataaaa
     5581 aggttgtatt ttttggagta ggtgctgtag gagctacttt tgcagaacaa ttttttaact
     5641 ctaaatatga ttttaaaatt ctttgtgata atgaaagaaa gaaaagatat ttagaagaag
     5701 gatttataat aaatggaaaa aggtatgatt ttgattatgt aactaaagat gagtataaac
     5761 aagaggctga ttttataatt ataggtctaa aatataataa tttaaaagaa aatataaaag
     5821 aattagatgg attagttgga aaaaatacag ttataatgtc tctgctaaat ggagttgata
     5881 gcgaagagat aataggagaa agatttggaa ttgaaaaaat ggtatattca tatgttacca
     5941 atatagatgc aaagaaaatc aataataata ttatacacac tactaatggg ataattgtat
     6001 ttggtaacaa agataatagt gaagatagaa aaactaatat aataaccgaa gtctttgatg
     6061 atgtaaatat agaatatact ttatcaaaag atattcaacg agatatgtgg tggaagtaca
     6121 tggttaatat tggtgtaaat caaacttcag ctatacttgg tgcaccttat ggagtttttc
     6181 agagttctga gcatttaaga gaattagcaa aatctgcaat gagggaagtt gttgctatag
     6241 cacaagcaaa aaacatatct cttacagaag atgatgtgga acattcatta catagaatac
     6301 tagaacattc aaaagaagga agaacatcaa tgcttcaaga tgtggaagct catagactta
     6361 cagaagtaga tatgttttct aagaatatct gcaaacttgg aaaaaaatat aacataccta
     6421 ctcctataaa tcagactttc ttttatatga taaaagtaat tgaaagtaga ttttaaaggg
     6481 tctttgtaag aaatattgat aattattgat tttaaatttt attaatgtta tatactattt
     6541 aagttactaa atatttttaa ctcttcaatg aaaatagttg ctgcttttga taaagttata
     6601 tcttttggaa atatatatga gaaatgtctg ttgtagctgc ttccaagctc tatgacagac
     6661 agctcattgt ttagaacagg taagcttgtc actagattag agactattgt aattcctaaa
     6721 ttatttctaa cagattcttt gacagcataa ttacttccaa aaaccatcat actttttgga
     6781 ataatttctt taacacttaa aaacatatct aaataatctc tggttccaga accatcctcc
     6841 ctgactatcc atttttggtt ttgaagttta tcaaaactaa aatcttttaa taaatgactc
     6901 ttgtatggaa gtgctaaaac catcttatct tcaaaaaaat attcttgaat aaatgaaggg
     6961 gaagagcaag tgccttcaat aagaccaatg tctaaaatat aatccttaac atgagaagat
     7021 acgattgatg tattttttat aaaaacttct acatctatgt caggatactt tttagaaaat
     7081 aatgctagaa aatttggtaa aatatactct ccaatagtta aacttgcacc aatctttaag
     7141 tgaccagtta tagcatctga aacttggctt acatcatgat aagtaatatt taaaagattt
     7201 aatatttctt tagctctttt gtacaagatt tgtccacttt ctgttataaa aatggttttt
     7261 tgtttaatgg acctgtttat aagaacaact ttaaaataat tttctagatt ttttatgtgt
     7321 ttacttacac ttggttgtga tagatttaaa tattccccag ctttagtaaa gtttttgtat
     7381 tcaacaacag ctacaaatgt ttttaattct tcaaacaatt ttttgtctcc tttacctaat
     7441 gaaattttac attttttata tactttctac agataattat actatatata ataaccatta
     7501 aaaacattaa tcattacgat attatatatt tattttactt atggttatta caattatata
     7561 ctattgctat gaagaaaact atattaatta taaggtaggt atattatgtt aaagaaaaaa
     7621 agcagtttga cattaaaaac tataagggaa atattaccag ggctgtttgt atcagtatta
     7681 gtaggttata ttagtatatt tatttctatt ttaattccta aggtaggggc agcatcaatt
     7741 tctatatttt taggtatgtt tgtaggaaac ctatttttaa atcaaaaggt atttcaaaaa
     7801 ggttataaat tttcagagac agatttgtta tcatattcaa tagtactatt aggagcaaca
     7861 ctcagtgtat ctaccttaat agacttaaaa gtttcaggaa ttttatttat aatattgcaa
     7921 atgacaataa caataatagc tgcactttat ataggtaaaa aactaggatt tgaagaaaac
     7981 tttagatttc taatggcaag tggaaatgca gtatgtggtt cttctgcaat aggagcaaca
     8041 gttccagtgg ttaatgcgac agataaagaa aagggaattg cagtaactat tgtaaatgtt
     8101 actggtatat ttcttatgtt tttacttcca ataatatctc aatttttata tagccatgaa
     8161 cttgtaaaaa cttcagcaat gataggtgga actttgcaat caattggtca ggttgtagca
     8221 agtggagcaa ttgttggaga aaatgtaaaa gatttagcta ctatttttaa gatagtaaga
     8281 gtaatatttc tagttgttgt tgtattagta tttggacata tgaaaaatca atctaatagg
     8341 gaaattcttg aagaagaaaa ggatgagata aaaaagaaaa aagtgactat accatggtat
     8401 gtaattggat tctttataac ttgtgcactt ttttcaatga acattatacc acaagaagta
     8461 tctgtgttgt gcaaagaaat aagtaacaaa ctagaaataa tagctttagc agcaattgga
     8521 ttaaaggtta atgtaaaaga tttagtgaaa caagggaaag aagtttcttt atatggttta
     8581 tttgtaggta cagtacaagt tgtatcagct gtagttttaa taaaaatatt tatatagcat
     8641 aaaaatagta tttgtaatat taatttgatt aattaaaata atatgatact aataatttat
     8701 aatataaata aaaaatgaat ttctataagt ttaagtgggt ataaacttat agaaattcat
     8761 tttttattta aggtaaaaat ttaaagtaaa atattatttt acataataat aaccttataa
     8821 attttgaaat tacttatctt tgtgttaaaa taagtaaaat gcacatatta gagctaagtt
     8881 tttaagtaaa taaacaaagt gaaaatataa atattaagga gatttggtat atgaatgttt
     8941 atgaaaaagg taatggagtt atactagaag gagtaacaga ttttgaccca gtacatatat
     9001 ttgagtgtgg tcaatgtttt agatggcaca aacaagaaga tggctcatac actggagttg
     9061 caaaggggag aatattaaat gtcaaaaaag agaatgataa aatatattta aataatacta
     9121 atttaaagga gtttaacagc atttggtata attactttga tttaggaact gattatacag
     9181 aaataaagaa taagctaaaa aatatggatg aatatttaaa taaggctact gaatttggat
     9241 ggggaataag aatattaaga caagatggat gggaaatgct tatatcattt ataatttcct
     9301 ctaataatag gattcctatg atacaaagag ctatagaaaa tttatcaaga aagtttggta
     9361 agtatatagg tgaatatgaa ggcaatgaat actatgcatt cccaacacca gaagaactaa
     9421 ataaggcctc acaagaggaa ataagagcat gtcaaacagg ttttagagat aagtacataa
     9481 aaagtactac acaggcagtt atagagaata atgatgaggt atctgaatat acaaatttaa
     9541 gtacagagga ttgtagaaaa gagcttttaa aatttaatgg agtaggacca aaagtatgtg
     9601 attgtattgc attatttggt atgcaaaaat atgattcttt tccagttgat gtttgggtta
     9661 agagagttat gcaagaattt tatatagatg aagacatgag tttacctaaa atgagaacct
     9721 atggaatcga taaatttaaa gaaatgtctg gatttgctca acagtactta ttttactatg
     9781 caagagaact cggtatagga aaataaatta agggggagaa attatgggag taataggaac
     9841 aatatttctg ttctatttaa ttatatcata tctagctgga gccataattt ctgtgattat
     9901 attacttgaa aatagagacc ctgcaaaaac aatgtcttgg ctattgatgt ttattatatt
     9961 tccaggtgta ggtttaatga tatatgctat ttcaggtaga aatattagaa aaagaaaatt
    10021 atttaagact caaaaactag ctaataatat aaaagaaaag aaactatttg atacattaga
    10081 aaagataaca gaaatagttg aattagaaaa agaatctata aaacaaaata agcttttaag
    10141 agatgaagag gatggtagtt atagaaaaag ggtaataaat atgttgttaa agactggtat
    10201 gtttcccttt actaaaaata ataaagtgga tgtatttgta gatggaaatg aaaagtttaa
    10261 aagacttata gaagatataa gagaagctaa agaccatatt catttagagt actttatcat
    10321 caaagatagt gaaataggta gggtgttaaa agaagaactt ataaaaaagg ctaaagaagg
    10381 cattaagata agaattttat atgatgatgt aggatgctgg agattttggt ttaatagaaa
    10441 atttttccga gaaatgaggg aagtaggtat agaaattgca gcatttttac ctacaaaatt
    10501 tcctataata ggagggaaat taaactatag aaatcacaga aaaattgttg ttattgatgg
    10561 aataattgga tatacaggtg gaataaatat aggagatgaa tatttaggca aaaatgataa
    10621 gtttggatat tggagagata ctcatattag gattaagggt atttctgtat acatgcttca
    10681 aatgacattt ctgattgatt ggtattatac gacaaaagaa gtattagtga ctaaaaatta
    10741 tttcccaagt gtagggaatg ttggtgaaag tatgatacaa gttgttgcga gtggtccaga
    10801 cagtgactgg gaagatattc attatgctta tttttctgca atatgtcagg ctagaaaaaa
    10861 tgtttatata gaaactccat attttattcc agatgaaagt ctactaaaag ctataaagag
    10921 tgctgctctt agtggagttg atgtgagaat aatatttcct aaaattgcag accacaagat
    10981 agtaaatata gcatcttatt catattttga agaaatatta agagcaggtg gtaaagttta
    11041 tttatataat aaaggattta tacattctaa agtcgtaata atagatgata aaattgcttc
    11101 tgcaggtaca gctaatatgg atttaagaag ttttatgctt aactttgagg taaatgcatt
    11161 tatctatgat gaagaagtta ttagggtaat gacagatgat ttctttgagg atttaagtta
    11221 ctgtgaagaa cttaatttgg aggtatttaa gaatagaaat atcatacaaa aaatcaaaga
    11281 atctgtagca agattatttt caccaatatt ataaaaaatt attaaaaaag gtattgaaaa
    11341 ttatgtgtga ttggtatatt ataataattg ttagcactct attagataga gtgataataa
    11401 ataaaaagta tatatataaa tctacatata taagtagtat taatttaaaa ataactaaaa
    11461 acataggcga actagtctat acttaatatt ttaggaggcg tgaataatga aaataagacc
    11521 attagctgac agagtagtaa ttaaaaaagt agaagcagaa gaaaaaactg caagtggaat
    11581 agttttacca ggagcagcta aagagcaacc tcaaatagct gaagttgtag aagttggacc
    11641 aggtggaata gttgaaggga aagaaataaa aatggaattg acagtgggag ataaggttat
    11701 attccaaaaa tattctggaa cagaagttaa gatagaagga caagaataca caatactaag
    11761 acagagtgat gtattagctg ttattgaata aatatagaat aaatttatta ggaggggttt
    11821 aaaatggcta aagaaattaa attttcagag gaaacaagaa gagctttaga agctggtgta
    11881 aataaattag cagatacagt aaaagtaaca ttaggaccta aaggaagaaa tgttatatta
    11941 gataaaaaat ttggttctcc acttataact aatgatggag taactatagc aaaagagata
    12001 gaactagaag atagatttga aaatatgggt gctcaattag ttaaagaagt agcaactaaa
    12061 actaatgatg tagctggaga tggtactaca actgctacag ttttagcaca agctataata
    12121 agagaaggtt taaagaatgt aacagcaggg gctaacccaa tacttttaag aaaaggaata
    12181 caaaaagcag taacagtagc agtagaagaa ttaaaaaatc aatcaagaat agtagaaaca
    12241 caagaggcta tatctcaagt tgcttctata tctgctggtg atgaagaagt tggaaaatta
    12301 atagcagaag ctatggaaat agtaggtaaa gatggagtta taactgttga agagtctcaa
    12361 actatgaata ctgaattaga tgctgttgaa ggtatgcagt ttgatagagg atttgtttct
    12421 gcatatatgg ttacagatgt agataaaatg gaagcagttt taaatgaccc atatatatta
    12481 attactgata aaaaaatatc taacatacaa gaattattac cagttcttga acaaatagtt
    12541 caacaaggta aaaagttatt gataatagct gaagatgtag aaggtgaagc attatctaca
    12601 ttagtagtaa ataaattaag aggaacattt gatgtagtag cagttaaagc tccaggcttt
    12661 ggagatagaa gaaaagaaat gcttcaagat atagcaatac ttacaggtgc tcaagtaata
    12721 tcagaagaat taggttatga tttaaaagaa gctgatttat ctatgctagg tagagcttca
    12781 tctgttaagg taactaaaga aagtactaca atagtagatg gttctggaga taaaaaagct
    12841 atagaagata gagtaactca aattaaacat caagtagagc aaactacttc agattttgat
    12901 agagagaaat taatggaaag attagctaaa cttgctggag gagtagctgt tgtaaaagtt
    12961 ggagctgcta cagaagtaga gttaaaagaa agaaagttaa gaatagaaga tgctcttaat
    13021 gcaactagag cagctgtaga agaaggaata gtagctggtg gaggaactgc ttttgttagt
    13081 gttataccag caataggaac actaattgag agtttagaag gagaagttaa actaggtgct
    13141 caaatagtta aaaaagcgtt agaagagcca ttaagacaaa tagctataaa tgctggtctt
    13201 gaaggtgctg taattgtgca aaatgttgtt aattctgaag cagaaactgg atttgacgca
    13261 ttgaatgaaa aatatgtaaa tatgatagaa gctggtatag ttgacccaac taaagttagt
    13321 agaagtgctt tacaaaatgc tgcatcaata gcaagtactt tcttaactac tgaagctgct
    13381 gttgctgacc ttcctgaaaa agaagatgca ggaatgccag gaatgggtgg cggaatgcct
    13441 ggtatgatgt aatttaaaaa atttaaaata taaattttaa tataaagaac tatttcgact
    13501 tagattttaa actctaagtt gaaatagttc ttttgtatat atctaatttt tgtatatcta
    13561 taaattcaat acacatattt agataatcag tatctagttt gtaataatat tttagagtag
    13621 tattgttatt ttttaggtta tgattttaga tatatattaa ttaaggtata attgttgata
    13681 gagaataaat caaatttaaa aggatgcgat aatatggact taagaaaatt gcttgaagaa
    13741 gttaaaaatg aaaatattga tatagatttt gcgttagaaa agttaaaaga tttaccttat
    13801 gaagaccttg gatatgcaaa tatagatcat catagagaac taagaaatgg gtatccggaa
    13861 gttatttatt gtgaaggtaa gagtgatgag catattttag ggattattga taaaatgaat
    13921 caaaaaagtt ctaacatatt gggaacaagg tgcagaaaag aaacttttct gaaaattaaa
    13981 gaaatctata atcatgctga gtatgaagag gcgtctaaaa ttcttaagat tcaaaatcat
    14041 gatatagaga atataggaaa aggtaaaatt gtgattgcta cagggggaac ttctgatata
    14101 ccagtagcag acgaagctta tcatacagct aagtttttag gtaatgatgt tgatagaata
    14161 tatgatgttg gagtggcagg tatacatagg cttttaaata aaagacataa aatagatagt
    14221 gctagagtaa tagtagccgt tgcaggtatg gaaggtgctc ttgcaagtgt aattggtgga
    14281 ctggttgatg ttccagtaat agctgttcca acttctgttg gatatggagc aaattttgga
    14341 ggattagcag cattactagc tatgttaaat agctgtgcat ctggaatatc tgttgtaaat
    14401 attgataatg gatttggagc aggatattta gcatcaatga taaataaact gtaaatatat
    14461 tgatataaat taaacaaaac tatataataa aatttaattt ctattatatt tttactatta
    14521 aaattgagga gacatatatg agtgaaaaaa tactttattt tgatataata agcggtatat
    14581 caggggatat gacccttgca agtttattaa atttaggtgt gcctaaggaa atttttttgg
    14641 aggaaattca taaactaaga atgagcgatg aatttaatat aaacatcagc tcgaaaactg
    14701 aaaatggaat agtaggtaca aaagtagaag taattacaaa agaaaaacat acacatagaa
    14761 atttagtgga tatatttgag gttatagata aaagcactct aaacgaaaat gtaaaatcta
    14821 aagcaaaaaa aatatttatg gtaattggag aagcagaagc aaaagttcat ggaactacaa
    14881 tagacaagat acattttcat gaggtagggg ctatagattc aattgtagat atagtaggag
    14941 cttctatatt agtcgactta ctttgtgtag ataaagtttg tgctagtaca gtgtctgttg
    15001 gttcaggatt tgtaaaatgt gaacatggaa ttatacctat acctgcacct gcaactatag
    15061 aaattttaaa aggtgtgcca attaaattaa ataatgtaaa tggagaatgt acaactccaa
    15121 caggagctgc aatcataaaa gttttgtgtg atgagtttgt agatgagctt gaattttctc
    15181 caaagcaaat tggatatggt ataggtcata agaagtttga agtaccaaat atactcagaa
    15241 ctgtattagg tgaaaaaaaa aagaagaatt aatttatgaa attagtgcaa acattgatga
    15301 tatgtcttca gaaatctatt cttatttgta tgaaaaaata ttagatgaga aagcattaga
    15361 tatttataca gagagcattt atatgaagaa gaatagacca gcaacaaaac tatcaatttt
    15421 atgtaatgaa tcagatttag aaaaatttat taaaatatta cttttagaaa ctagtacttt
    15481 tggagtgaga tataatgcat ataatagaga aattctagag agaaaatttg taaaattaga
    15541 tacaaaatat ggagtggtta cagtaaaact agggtattat aaaggaaaac tagtcaaggc
    15601 tactccagaa tataatgaat gtaaaactat agcaaaaaat gaagatattc ctataaaaaa
    15661 agtgtttgct gatataaatt ttagaataaa agaagaattt agtgaaaact tattgacata
    15721 agtaataaat tttgataaat taatattcag ccttattaaa taaaagtaaa cagaaaaaaa
    15781 tatataaaat ataaactcac tcgtatatgc tcagaaatat ggtctgagag tctctaccaa
    15841 gataccgtaa atatcttgac tatgagtgaa attattatac caaaagacct agttaagtaa
    15901 atgtgtaggc tgagttaaac tccctatgtg ttggctataa gataaaggta ttttggaagc
    15961 ctgtatagta atttcacttt tgctagaaat tatggaagtt actatacagg ttttttgtgt
    16021 aaaaagattt aatataattt agactgtatt gtgttaaaat aaatatataa aaactatatt
    16081 atacaaggag agatgtaaat ttatgaaaca tgaattagta cttgtaatag attttggagg
    16141 acaatataat cagttgatag ctagaagagt tagagaaaac aacgtatatt gtgaaatact
    16201 tccatgtact gcaagcatag aaagaataaa agaaaaaaat cctaaaggga taatatttac
    16261 aggcggtcca aatagtgcgt atttagaaga ttctccaaca atatcaaaag aaatatttga
    16321 actaggagta ccaatacttg gaatatgcta tggtattcaa ataatgtctc atgtattggg
    16381 tggagttgta agaaaaggta ataaacaaga aaaagaatat ggaaaaacag ctataactta
    16441 tggaaaatca tcattatttg aaggaataac tacaaatagt gtatggatga gtcatacaga
    16501 tttaatagaa aaagttccag aaggatttac tatagttgca aatactaatg attgtccagt
    16561 agcagctatg gaaaatgtag aacgaaacct atatggagtt caattccatc cagaggtaga
    16621 acactgtttg gaaggggaca agatacttac aaacttctta tataatattt gtaaagtaaa
    16681 aggtgactgg actacagatt cattcataga agataaaata aaagaattaa aagaaaaaat
    16741 aggcgataaa aaagctttat gtgctttaag tggaggagtt gattcttctg ttgctgcagt
    16801 tcttattcat aaagcaattg gggacaactt aacttgtata tttgttgacc atggtttact
    16861 tagaaaaaat gaaggaaatg atgtagaaag aatatttaga gagaaatttg atataaattt
    16921 aataagagtt aatgcagaag atagattctt atctaaatta aaaggagttt ctgaaccaga
    16981 agctaagaga aaaataatag gtgaagaatt tataagagta tttgaagaag aatcaaataa
    17041 attaggaaaa atggatttct tagtacaagg aacaatctat ccagatgtta tagaaagtgg
    17101 acatggaaat gctgctacta taaagtctca tcataatgtt ggtggaatcc cagaagatgt
    17161 agacttccaa gaaattgtag aaccattaag agagttattt aaggatgaag ttagaaaaat
    17221 aggattagag cttggtatag aagaaggatt aatatttaga catcctttcc caggaccagg
    17281 tctaggaata agagttatag gtgatgtaac taaagaaaaa tgtgatatat taagagaagc
    17341 tgatgctgtt tatatggacg agcttagaaa agcaggttta tatagagaaa tatggcaagc
    17401 ttttgcaaca ttaccagatg ttaagactgt tggagttatg ggtgatgaaa gaacatatgc
    17461 ttatttagtt ggtttaagag cagttacttc ttctgatgga atgacttctg actggtataa
    17521 gatgccttat gatgtattag aaagaatttc taatagaata ataaatgaag ttgatggagt
    17581 aaatagagta gtttatgata taacttctaa gcctcctgga actattgaat gggaataaaa
    17641 tatagaatgc caaaaatcca cataaataca ggatttaagg tctctcttac ttctatgtta
    17701 tactggtttg atactatcaa tatttgccag tatcttatgg attaataagc aaaaaacatc
    17761 ctgctgaatg ataaattcag cggggtgttt ttattttgtt ttagaactcc tttgctctga
    17821 aactcgaagt atttaacact gatgattttg ttatctttta gttccttttg tgtcatgctc
    17881 tgaaagacaa attgatacaa taaaaaagta atttcttaat cattgatgga gatatgtatt
    17941 tatgttactc ttgatggtaa tttgagtaat attgttcata gctttttata ttcacttaat
    18001 tagagaatca tttggtataa tcataaataa acttgatgat ataatagaaa agatttataa
    18061 ttattagaag tatataaaag tattttatgt ctatgaaatg tatgtctata aaacatatag
    18121 atgacttttg tatattaatg tatgaatatt gatattatag aagaattgag ttgtcaatga
    18181 tactaaatca aatatttata gaagctggag ggattatgag aaataaaata attataggaa
    18241 ttactttttc acttgtatta ctaatgtctg gatgtgcttc aaaacaaaca gcagaaataa
    18301 acgtattagc aactactgat ttacatggag ttatacctta tgagttaact tcttatgtaa
    18361 aagaagaaag aaaaaaagat aaaaatctaa ctttagtaga tgcaggagat ttttttgata
    18421 gtggagaagt gtttggttct agcatggata gttattttag tgctaggtat aaggatattg
    18481 ataataacac tgagcgttac attgagacac ctatttcaaa agatatgaaa gaggtaggtt
    18541 atgatgttgc agttcttggg aatcatgaat ttatatccaa caataaattt tatctagata
    18601 atatgatatc tgattttgag aagcaaaaaa taaatatttt atcagcaaat acgtataaaa
    18661 aaaatggaga cagttataca aaaccataca taataaaaaa tattaataca cctgaaggaa
    18721 acgtaaaact aggaatttta gggcttacta taaaagaagt tggtgagagg aaacagtgga
    18781 aagatggaaa acttgttgac tctaagtctt tagaactaaa agaccaagag ggctacaatg
    18841 gtgaattgta tatgaatgat ttagtagaag atgctaaaaa gtgggttagt ataatggaaa
    18901 aagagaagcc tgacattata gttgctgtgg cacatacagg tgagaaacct aagaaaccta
    18961 gaaacccagg aaatagaata caagacttag ctcaacaagt tgatggaatt gatgctatag
    19021 ttgcaggtca taatcatgtt caaattgagc aacatgatta taaaaataaa tcaggagaaa
    19081 atgttatagt tactgagcca ggaaagcatg gagaatgtat ttctaagatt aattttaaat
    19141 tagaaaaaaa caatgataaa tggaatatag ttgacaaatc tagtaaatta attaaatttg
    19201 aaaaaaatca agaaagtgat aaatttggag aatttatttc ttcaattaat aaagtaagta
    19261 aagataagaa agaaataaga ttgtcagaag taaagactcc tgaatgggat aaaatatatg
    19321 tttttaaatc tggaacatct gcagatgata tatataaaac aataggatat aaatggagaa
    19381 atatatcatc tccaaagggt gatgacatgg tgcagattgt tgtaatgaat aataataagc
    19441 ctgttagcta tgtatatggt gaagctgaag ttatgccaat taatataaaa tgtgatgaat
    19501 cagagtataa agataatgtt gtaactattt atcctaataa aaatgataaa tttaaaattg
    19561 aaaaaggtaa acaagaatat agtattaatt taaaacatat acaatagaaa cttttatctt
    19621 atttaagttt agattgtact taaaataaag tgtgtattat tttaaaaatt gatgataatt
    19681 aaaatcacaa cattaaggaa tagtacacac tttttatgct aaaaaataga atgttaaaaa
    19741 attaaacctt taaattaaag tacattgctt atattatcca atattatctt caaaggttgt
    19801 gtgatatttt tatccttatg taatattgct aacattttat tttccatatc tatcccatct
    19861 aattcaatca atcgcaattt tttatgtaag agtgaatcta ataataaatt ttctggcaaa
    19921 atggttatac ctaaccctgc ttctgcagct ttaataagtg caaaagaatt aacactttcc
    19981 caaacaggat aagctttttg gtttgaaaga gataatacac tgtccaaagt attgcgaatg
    20041 gcacttcctt gctcacgtaa caataacgga aacgtgcata actgtcgtag agataaggct
    20101 tgtttagtat tggtggtggt tatgcaatca tacctttaat ccaagagaaa gtagtaaatc
    20161 aaactggatg gataagtgag aaaatgttta cagatattat aactatttca caaatgacac
    20221 cagggccact ggctgttaat acttctactt ttgttggact tcaaattgga gggttttggg
    20281 gagcatttgc agccacttta ggttgtgtat tatgtggtgt agtaatagct ctatttttat
    20341 ataatttttt tcaaaagcat cagaactcta cttatatatt tgaaatatta aatggtctaa
    20401 aatcagcttc attaggttta attatctcag ctgcggctac aattataatt cttgcattat
    20461 ttgggacgaa taaagtgtac ttcaattcta tatttgagat gctaaactgg actgcactaa
    20521 taatcttttt tgttacacta ctgatagtac gtaagtggaa aattaatcct attgtgatta
    20581 taataataag tggtatactg ggaatatttt tttatttata agtttttgct aagtaaagga
    20641 tatctatttt tttatatctt aaatccaaat agccaataaa gtaaggctat ttggattttt
    20701 aataaattct gaaaaaataa tcagaaaccc atataacatt taaattatat cactggtttt
    20761 ctacaaacca atcataagct tcttggcaaa atttctcact atctatatag taactattat
    20821 gaacattatt aatttcaaca agtttctttt tacattttag tgaattataa tatctatcaa
    20881 aatattgggt tttaaaatat ctatcatgaa gtgtcttaaa gacaagagta ggtatgttga
    20941 ctccatcttt tatcttacta tcttctgcaa taaagcattc taacattgta ttaattatct
    21001 ttgtactatt tttccaaata tgaggatttt tcttttcaaa tattttataa aattcattat
    21061 catagacttc attataacct ttatatttag gtggttttac tgttatgtga aaattaggaa
    21121 gaaacttaaa gaaaaagttt aataatttaa cagtgagata ggtatagcct actcctgttg
    21181 tgtttttata atcaacacaa gcaaactgag caaaactttt taacttattg tctgaacatg
    21241 cataatattg tgcaaacata ccaccaattc ccgtattccc atatagatgt atgttggaag
    21301 aataattttt ttctatgtat gtaatgacag aatctaagtc ttttacaatt gaattgcgag
    21361 aaaaatcttt ttcatatcca tcacttttcc ctgttcctga aaaatcaaaa gcaaatatat
    21421 ttacattatg tttggcaagt ggttcatatg ctttttgcat ctcaagcgta gtgacaatag
    21481 gagtatgtgt aacaacaata ttcatggatg attttctggt aataaatgag ttacaaaaaa
    21541 tcattgtatc tccatttggt atttttatat gtttccaact catttgattt tcctttctct
    21601 attataaaat ataataattt ttatgttctg ttttatatat tattcatatt ttatcacaga
    21661 taatatacta aagttaattt ttttattgta cacctattat aataattctt ctataatata
    21721 agacattcta taattttata tttaagttca tgtgtattta attgttttta tataaaatgt
    21781 ccagtaatac tatttggatt tatttttatt tcatcaggag ttccagaagc aacaatttcc
    21841 cctccattta ttccactgtc tggacctata tcgataatcc aatcagaagc acgtataacc
    21901 tctgtattgt gttcaataat taaaagtgaa tgctgttcat ttacaagttt tctaaaagta
    21961 attaacaaat tttctatatc tttaaaatga agtccagtag taggctcatc caatatgtac
    22021 agcattcgtt ttttagtttt tgtccctaat tcttttgcta attttatcct ttgagattct
    22081 cctccactta atcctaatgt attttgtcca agctttagat atccaagtcc cacttcatct
    22141 aataaggaga gttttctata tatatcagag caatcgctaa aaaatgtttt tgcttctgct
    22201 acttccatgt caagtatatc tgcaattgtc tttcccttgt attgtataga cagtatttct
    22261 ttttgatatc tcttaccttg acattcacta caagttaccc attggtctgc catatattgc
    22321 agaataattt ttgttttacc taatccttga cagtttgggc attgcccctt tttgctatta
    22381 aagctaaaat agctttcatc caataatttt tgttttgctt gtggagtttc agcaaataga
    22441 gaacgaattt catcaaaaat attgatatat gtagcaggtg tagaacggct agattttcca
    22501 ataggtgttt ggtccattaa gataaaatca tcaatagcat caaatccagt aaattcagta
    22561 taatttttat ctgggataga tttttgtttc aatttttctt ctagagcagg aagtaaactg
    22621 tgaaatacaa gagagctctt accagaacca ctaacacctg taatagagca catacagttt
    22681 aagggaattt ctacatcaat attttttaag ttatttgcat ggcatccttt taagcttaac
    22741 catttacttg gtatagtttt attttgtgaa cctgttttat tgtatgtact taaatattta
    22801 cctgttatag agtttggatt ttttataatt tcttgggttg taccttctgc aataatgaac
    22861 cccccttttg taccagcact tgggcctaca tcaataatat aatcagcaga cagtattgta
    22921 tctttatcgt gttcgacaac tataacagta ttacccttat ctcttagctc ttctatcatt
    22981 ttgatgatta aattatggtc tcttgggtgt aaaccaatag atggttcatc taaaatgtag
    23041 gttaagccta ctaattcact tcctagctga cttgataatc gtactctctg cagttctcct
    23101 ccagataaag ttggagatgt tctatctgtt gatatatagc taagacctac ctttaataga
    23161 taactaactc gaatttttaa ttctgttaag atatcattta ccaaagagag ctcatttttt
    23221 tgaagttgat ttggtaattc acacaaccaa ttccataact gttctattgt catttttgtt
    23281 agttcaggga agcgataacc tttgattgta gtgaatctag cctcaatgca cagcaattcg
    23341 ccaccacatg tattgcaagg aattttgttc atatattgat gcaaggtatt attttcagaa
    23401 ctagattctc taaaaagtct ttgaatgtgg ttgattgctc ctgaagcagg tcttctaatc
    23461 tttgattctc gtccctttga ttcaaagcta aattcatata tcttatcgtc tgtaccatat
    23521 aagattgcat ctataaaatc tcttggtaat tcattccaag gtatatctag gtctatattt
    23581 ttatcttttg ctattgcata taattcacct accatccaat taccatttgg ctttttcccc
    23641 ctcaattttc caaaatagta agtggctcca tctaatattg aaatagttgg gtcttttaca
    23701 atcaattgag gattaacttg ataggtaaaa cctaatccat tacaatcgtg acaagctcca
    23761 gtatgggagt tagcattgaa ggttgaagta tttatcttag aaaaaatagt ttgacaatgc
    23821 ggacaataat tttttgtatt attctttttt tctaatggaa ttttacaata tggacagtaa
    23881 ctttttccga ttgatacaaa taacattctt aaataatccc aaatgcttgt aagagtacca
    23941 acagtagagc gaggattttg gcttcctttt ttctgttcga tagctatagt aggggttaat
    24001 ccctgaagta aatcaaattc cggtttttga agctgttctg aaagttttgc tccattactt
    24061 agattttcaa aataacgtct ttttccttct tcataaatcg tatcgaaagc taaagaagat
    24121 tttccactac cacttacacc tgtaataaca gttatttgat tttttggaat tgtaacctca
    24181 acatccttta aattgttttg ccttcctcct tttaaataga tagagctttt attatgacaa
    24241 agtgaatttt taattgattt atttattgat tttgtaaatt tgaatttctt ttctagttca
    24301 agaggattgc ctaattcttt tatttccgaa atacctaata tttttaactg agaaaagatt
    24361 gatttcaaat aatcttcagg caatttttct attaacaaat taaggtcttc tatttttata
    24421 tttccgcttg ataatatttc tgccatttca tctaataaat ttttttctgc tcttgttaat
    24481 tgattgtatt ttttggaact taattctgtc cataaatccc atggaagcaa ttctaaacca
    24541 atcatattat taaagtcatg tagaagatta ccctttacag aatgccaacc tttccaattt
    24601 ttattatatc caaagtcatc tggatgagca tgtccttgcc gacatcttat ccaggcttct
    24661 gaaccagtta aaaaaccatc tgtttttccg acatcatgag tgtcaaaatt aatttgaaaa
    24721 taattttttt gtatgtcatc tatttgtgca tctattctta tccatctctt tgttagagta
    24781 tcatagtatt ctacaagcca gtggtcttca taagtaagct cactttcaaa ataattagca
    24841 aaacctgctc tcattctagc ctcaaaacct cgatatctta ggagcgatac aagaagtaaa
    24901 gaataatctc tgcacattcc aacaagcctt tgttttgggt ttcttggaat agttagtggg
    24961 ttagaattta tttcaaatat tctttttaac atttgaggaa ttgtacgaag caattcctca
    25021 tcagattgtt gcttattaaa ggatatacca tataattttc cttggtctcc atgaattact
    25081 aggctttgaa ccacttttaa taactcaatt ggagtctttg gtaaatcttg atatttattt
    25141 tcaaattttt taggatttgt gatgatgctt tgatttttat agttatttat tttcatttat
    25201 atttcacctc tctaaatgga tgatataccc tgacatggtg tcatatgcaa ggacaattta
    25261 aattttttgt ccaaaattta aaaataaata aaaaatgata cagtgataaa ttgtaataca
    25321 aatatagttt caaattgcat ataatataat tatttaataa aaccattatc tatggaatgt
    25381 taatgaaatg ttaagatggc attcttgaat attaagaaat agaatactat aatatagtgt
    25441 gaaggattct tagtaaaaaa atttgtaagt gttaatatac attaaaaaat aggtttataa
    25501 tttggaggaa aaaaatgaaa ttatcaaggt ataagaaaaa tagtttagtt ctgtctataa
    25561 ttttatgttc acttattatt tcaatccttg taattactgt ttttaatacc gtaaaactac
    25621 acaatatatc taataaacaa tctaaaaact atttaaatga tgtatctaca cagattgtaa
    25681 tgaatgttga ttctaaaatc aaatttattt tatccgactt aagaataatg gcagatttta
    25741 ttaagcaata tgaaggtgat agtcgatatg agtatttatc taaaagaaag actagttata
    25801 agtattatga tgttggaata atagatttag aaggtaaagc tgagttttta agtggaaaaa
    25861 agtttgactt aaagaatact gcttcctata aaaaaggaat tgaaggtaaa gaatactcag
    25921 aagtaataga gtcaatgggt tttgttcttt atagtgtacc catatttaat gataatcatg
    25981 aagtatctag tattttaata ggtgtatcca ataaagaaag tatgaatgag attcttaata
    26041 tagataactt taatggagaa ggaactattg aaattattaa tagtaaggga aaacctcttt
    26101 tcataggaaa aaatactgaa ttaataaaag accttagcca taaatataag gattctacag
    26161 atgaaccttg ggcacaaaag atgcttaatg attttaaaaa tgataaatca gggaatatta
    26221 caataacctc atcaaaaggt gtacaatgct tattgactta tcatccaatt acaagaggtt
    26281 taaatgattg gcattttctt ctcatagtac ctgaagatgc tgtacttggt gaacttaata
    26341 agttaaattc ttttacaata acaatgacat ttataattac atgtattatt ggaattataa
    26401 cattaatttt atatattata agaagaaaat atgtaaatca aatagaaaat attgcatatt
    26461 tagatagtat aacaaatggc ataaattcaa ctaagtttag tatgctagtc aaacctttaa
    26521 tttcagaatc acctgatagt acttatatga tgatagcaat gaatataaaa gattttaagc
    26581 taataaatga ttgtttcagt agtgaaaagg gaaatatgac actaaaacat ctttacaata
    26641 ttttgtataa aaacattgat gaaaatgaag aatttgtgtg ccgtcatgat gcagaccttt
    26701 tctatttgct tattaagaat agaccagtgt tagaagccct ggagttatta tataaaatag
    26761 aagctgacac aaattatttt aataaaaata gggaaaatcc atacttttta agactatcag
    26821 taggtattta cacaattgaa aatcataatg aagatttaat tacaatacaa gaccatgcaa
    26881 atacagcaag aaaaagcttt aataaaactc atcaaagtga tttttctttt tatagtgata
    26941 tagaaagaaa acgattaata tctgaaaagg aaatttcaaa tttaatggaa aaagcccttg
    27001 aaaataaaga attttttatg tgcttacaac ccaaaattga tataaaaagt ggaaaaatta
    27061 gtggagcaga gtctcttgtg cgttggaaaa attcagaaaa gggcataatt tatccaagtg
    27121 attttattcc tcttttcgaa aaaagtggat ttatatgtaa gttagattta tatatactag
    27181 aagaaacatg caaattgatt tctaagtgga taaaagaagg taaagaagtg ttgagaattt
    27241 ctgttaatgt atcacgtcaa catctgaatg ataaattgtt tttagaaaaa tataagcata
    27301 tatgcaataa atacaatgta ccaacatgtc tgatagattt agaacttacc gaatctattt
    27361 tcttagaaaa tccagaagct atagatataa tagaggatat tcattctaat ggatttaaat
    27421 gttctataga cgattttgga tttgggtact catcattagg aatattaaag gattttaaag
    27481 ttgatataat taagttagac cgttcattct ttgttagcaa aaataatata gaccgtggta
    27541 aggtagttat taagtctatt attgaactta gcaaaaggct aggtatgaaa gtaactgctg
    27601 aaggaattga agaattagaa caagttgagt ttctaaaaaa tgtgggatgt gattatattc
    27661 aaggttatgt tttttcaaaa ccattattaa tttctgattt tgaaaatttt gcatatgaaa
    27721 ataataaaat taaaaatatc cattattaaa tgtacaagat aaaaaattta ggcaagttat
    27781 ataattaaaa atataacttg tcttttttat ttatatattt aataaaaata tatttgcaga
    27841 ttttgtaaaa ataattttca aatgttgtaa gttgtggcta aagctttatt gtattattct
    27901 gaatatagaa agatagttac tgtcttagat aaaaactgaa acatacataa taatataaaa
    27961 tttgctatat taataactct aattattttg gaaggaggga aaatatgata agtgggagaa
    28021 tgttactcat catgaatttt ctaaaaggaa aaaataagac aagttacaag gatattagca
    28081 aacaacttag tatagaagaa agaaaagtta gatatgatat aaataattta aatattgttt
    28141 tgcgttcatt aaacaagtca gtaatacaaa agatgaacaa aggtgtgtta attattcctg
    28201 atgacttcca agtagtatgt ataaataata acgaatatgt attttcctta actgagcgag
    28261 tatctattat gaaaattata tcaatgttta aaatagataa tctaaactta gaaaaattaa
    28321 gtaaagaatt tcaggtttct agaagttcaa ttaaaaatga ccttaatgtt ttgtcaagtg
    28381 aattagaaag aaaccaaata actattactt atgataaatc ttttaaaata agtggagaag
    28441 aagacattat atataagcaa agattgaaca tattaaaatc gtattcttat ctttttggta
    28501 aggaaaaatc tgatttaagt gtatttgaga agtatttagt gaatgtaatt gaaaaaatat
    28561 tcaaagggat ttatttgatt gatatatata attggtctat gaatttatta ttgcaaatgg
    28621 gatggacttt aaatgatgaa tcttttacat ggtatgtgtc aaatattttc ctttttacat
    28681 ggtatataca ttcagatgaa aaacatcctt tagaatctgc ttcattaagt gagccgtgtt
    28741 ttgaaaataa gtttgaccaa gaatttgaaa caattattca aaaaaagttg actaaggaac
    28801 aaatttttct gttaaagagt tttgtatttt ttacaaataa atatgcaagt ttaaacgagg
    28861 atatggattt gattagtaca gaaattatag ttcaagatct aatttacaat atgtcagaaa
    28921 tattagagat tagctttaaa gaagatatga ttctttataa aggattactc aatcatattg
    28981 ccccattgat agagagaatc aaacagaata tacagattta tgaggaatta cttcatgtaa
    29041 taccagataa atatcaatat gtacttgaag ctactaaggt ttctattcaa aaaaacagag
    29101 ttttaagtca aataaagaat gaaaatgaaa caatactttt ggcaatacat ttcttaggaa
    29161 gtatacatag aaatgagagg aataactaca aaaatatatt gttagtttgt ggatttggat
    29221 atggtgtaat tgctatgtta aaggatagtt tagtaaatga ttaccagata aatatagtag
    29281 agagtattcc atcatataag ttacaagatt ataaagaatg ggaaagtatt gacttcatta
    29341 taacaacatc aaagatagta atagacttgc ctaaaacaat tattgagata aatccatttt
    29401 tacaagaaga ggattatttg aagtttgaag aaaaagggat aaaaagaaag aacgtcttaa
    29461 ctaattatat gtcaataaaa aaaaggttgg actttttaga agaagatgcc caaaaaagag
    29521 tattaagtat tatatgtcaa gaacttggat acagtgatga gagaatatta tttagacaac
    29581 ttaatttaag tgatttaata gggattgata ccataaggat aattgatgga gatatgaaat
    29641 gggaagatgc tgtaaaattt agttcaaaca tacttgctaa ttgtgatttt gtatcagcac
    29701 aatatgctga gaatattata gatattttaa aaaatgtggg tttttatgct gtaaaagata
    29761 acgaatttgc tttactacat ggcaatgata gttcattagt caaagtaagt tcgataagtt
    29821 tattaatatg taaagaagca gttagttttg gagataaaaa ggttaaaatc atattttgcc
    29881 tagcaagcag agataaaaaa gagcaaattc ctgcaataat taatctaact aaaatggttt
    29941 ataagactaa ttttattgct atgttagaaa gtgcaaagac aaaagaagaa gcagaagttt
    30001 taattcataa atatgagaag gaggtcactg tatgaataaa ttagttaatg taaaatgttc
    30061 atttaaaata catgttaatt caaaagaaga agccattata tcgttagttg atgtagtgtc
    30121 aaaagaagga tatttaatag ataaaaatca atttttaaaa gatgttttaa agagagaaga
    30181 aacattgtcg acttatattg gacatggtat aggtcttcct catagtcaaa gtgtaggtgt
    30241 taaaaactct tgtattacaa taggaaagct tgatactcct attgaatgga cagaagaagg
    30301 agaaaaagtg gatttaatat ttttgatatc agtaacaaaa gacaatgaaa ataatcttca
    30361 tttaaagatt ttatcaaaat tagcacgttt attgatgcat gaaagtttta gaaatcaaat
    30421 tagatgttca gatgaacaaa ctgtttataa tcttataaaa gaaaaaatag aggaggaaga
    30481 ttaaaatgag aattttaaag tttttgcaaa aacatcttat gacagcaaca tcttatatga
    30541 ttccatttgt ggttgcagga ggaatattat ttgctttatc agtaactcta agtggacaag
    30601 ctgctgttcc tgaaacaggc tggttggcta aactgaatca aattggagct gctggcttgg
    30661 ctttattcat tcctatatta ggtggatata tagcatttag tatggcagat aaaccaggat
    30721 tagctccagg tatgataggt gcatatttag caaaagaagt aaatgcaggt tttataggtg
    30781 gtatcatagc aggatttata gcaggatttg ttgttttaca attaaagaaa attaaattag
    30841 ctcctacaat gagaacttta ggttctatat ttatttatcc tttgattggt acattgatta
    30901 caggtggtat aattgtattt ttaattggag aacctatagc ttcttttatg acatggatga
    30961 caaactggtt aaatggaatg tctggtgttt ctaaaattcc tttaggtgga atcttaggag
    31021 gaatgatagc ctcagatatg ggtggtccta taaataaggt tgctgctact tttgcccaaa
    31081 ctcaagttga cacacttcca tatttaatgg gtggagttgg cgttgcaatt tgtatacctc
    31141 caattggttt aggattagct acattattgt ttccaaaaaa atttagcaag gaagaaagag
    31201 attcaggtaa agcttcacta ttaatgggat gtgttggaat tacagaagga gctattccat
    31261 ttgcaacatc agatccagtt agagtaatac cttgtattat ggctgggtcg attataggaa
    31321 atattatggc ttttttatta ggatgtttaa atcatgcacc ctggggaggc ctgatagtat
    31381 tacctgtagt tgacaatagg ttggggtata ttgcatcagt attaactgga gcagtagttg
    31441 ttgcagtatt aatgaaatta gtaaaaaaag acgtgaaaga agatgaagaa attgaagaag
    31501 atttagatga atcaattgaa ttaatatttg aagaattata aatataaatg gaaggaattt
    31561 gtataaaaaa gtaaatataa aactataaat ttaaaggaga taagaatatg aaaattgtag
    31621 cagtaacatc ttgtcctagt ggagtagcac atacttacat gtcggctgaa tcacttgaat
    31681 tatcagccaa aaaatttggt atagaaataa aggttgagac tcaaggaagt tctggaatag
    31741 ataatgaatt gtcacttaaa gatattgatg aagcaacttg tgtaatattg actaatgatg
    31801 ttgagattag aaacatggaa cgttttaagg gaaagaaggt agtaagaatg agtgtatctg
    31861 agataattaa gaaatctgat gcattaatta agaaaataaa agatactttt caataaaaat
    31921 tgatttattt tacatataac tcaaagatgt ttttaaagaa atttttatta aaagcatctt
    31981 tgaaaatatc ttaaaaatag aataaaagga gatatagtca tgaaaaagtt accaataaag
    32041 actgtcgtac aaagattact aaacttacaa gaagagggga aaagtgcaac tcttttagga
    32101 attggtccta tgtcaccaaa cttgttacaa gcaagttttg aactagcaaa agatgatgat
    32161 tttcctttaa tgtttatcgc aagtagaaat caagtagatg cagatgaact aggtggcggt
    32221 tatgtaaatg gttggaatca ggaaactttt acaaaagata ttaagaaggt tgccgacaaa
    32281 gttggatttg atggtttata ttatttatgt agagaccatg gtgggccatg gcaaagagat
    32341 aaagagagaa atgaccattt gcctgttgat gaagcaatgg agttaggaaa aaaatcttat
    32401 ttagcggaca ttgaagcagg ttttgaccta ttgatgattg accctacaaa agacccattt
    32461 gaaattggaa aagtaattcc actagatgta gtactagaaa gaactgtaga attaattgag
    32521 tattgtgaaa acgagagaaa aagacttaat ctaccagaca taggttatga ggttggtaca
    32581 gaggaaacca atggaggtct gacttcaact gagacgtatg aaacatttat aactagacta
    32641 aaggttgaac ttgataatag aggtttgcca atgccaacat ttatagttgg tcaaactggt
    32701 actttaacta gaaagacaga acaagtagga atttttaatt ttagaaatgc ttatgattta
    32761 gctcaaatgg ctaagaaata tggagttggt ttaaaagagc ataatggcga ttacttagat
    32821 gatgtaactt tattagaaca tataccatct caaattattg ctacaaatgt agctcctcaa
    32881 tatggtactg aagaaactag ggcatattta aaattagcag aagttgagtg taaattagaa
    32941 aaagagggct tagttgagaa aacttctaat atcagacatg ttcttcttgt taatgcaatt
    33001 gaatgtggac gttggagaaa atgggtagta ggagaacaaa aaaatctaac tactgaagag
    33061 atatttaagg atgaagtttt atctaatgaa atactagata ttgcaggaca ttatacattt
    33121 aataatgatg atgttaaaaa agaaatagaa gtgctttatg ataatttaag taagaataat
    33181 attgatggtc aaagatttgt tgtagaccat attaaacgtc ctttgcgtga ttatgcagaa
    33241 tgctataact taaaaggtgt tactacgagg attttaaata aataatagga cttatagtgg
    33301 tgagtattgt tatactaggt atttatagtt ataattatac ttaccactat taaatttagg
    33361 aggactaata ttttgattaa tttaattttt gatgtagatg atactttata taatcaatta
    33421 acgccttttt acactgcata taataaagtg ttttcttcaa taaaggatat ttctattgaa
    33481 gatttatata tgagtagtag aaagtatagt gatgaggtat ttcatatgac tgaaaatgga
    33541 gaaatgccaa tcaaagaaat gcatatttat aggataatga aagcttttga ggagttggga
    33601 aattctatta cagaaaaaga tgcacaaagt tttcaagatg agtatatata tcaacaatca
    33661 caaattacac taataccaga agttgagagg attttaaatt tttctaaaga aagaaatatt
    33721 aatctaggaa ttataactaa tggcccatca aaccatcaaa gaatgaagtt aaagcagtta
    33781 aatattgaaa actgggttga taaaagtaat atttttattt ctagtgaggt tggattttct
    33841 aagccagata ctaatatttt tagagtagct gagaatgtta tgaatttaga tagagaaaat
    33901 acatattatg tgggtgattc ttatagaaat gatgtattag gtgctaaaaa agctggttgg
    33961 aagagtatat ggttgaacca tagaggatat gaggtagaag aattgtttta taaacctgat
    34021 tttgtaattt tggaacataa ggatttgata tctttattta taaaaatatg tagttataaa
    34081 aatatgtagt aaaaaaatgt taataataga attgtataaa ataattaata ataactatat
    34141 aaaattatat gtttagattg tgagcaatct attttatatt aaaaatagat tatttagaag
    34201 aaggggaaat cttaaaaaaa tttgaatatc ccctttttat tatttaatta aaatgaaata
    34261 gttagaaata tattttattt agtttagtca ataacatttt gggattcttt ataactacat
    34321 gttaactgtt gaataggttt atcatatggt ttaacccatc ctttattaat acttaattct
    34381 gtaagtgctg tgtgaccttc taccatttga gttaatgaag ctgaatatat agctcttaat
    34441 tcaggtgtac tacttgtcaa tgcagaatta agatacatat tggcagatgc tttagcagat
    34501 gaaagcatac ttaacattat tactttatcg tctatataag tattgttctt aattagattt
    34561 tctatcatat ttctcatgtt atctatacct cctcagaaat tagtatttca ttttcaacta
    34621 taaattgttg tatagcttta attcttcctt ctgttgctaa tatactagat tctgattgtc
    34681 tttttaggtc ttcatctgat attaacatat ttattgctct ttgcataagt aatccgtcag
    34741 actccatttt tataattgct gctaaagata ttttttcagt ttctgaaagt attctcattt
    34801 aatcatctcc tatattgtaa tttatgcttt aatagtatgt ctttcttttc aaggaaataa
    34861 ccattacact tttttacagt attttatatt ctctttatat caaaatttag tcgacctaat
    34921 atattaatat aattttgtag gatatttatc attaaatatc tataaaataa attatttgaa
    34981 tattcataaa gcaaaattta tttgtatgta cttcttcaag agtcaatatg agttttttca
    35041 aaaaattaat ttttatgata atcattaagg gctatatttt tcttaaagtt gtgattatca
    35101 ttattaattt acaataaaat tgccgaaaag ccctctcctt taggtgtagg gatgaaaggt
    35161 aaggtcttaa tataaaaaaa gttagaataa tgcttgtaat ctaataccgc tactgatacc
    35221 ctatatttag gcggtgttag tttttatgta taaatctaac aaaaatatag tttatgattg
    35281 caaatatcat attgtatttt gccctaagta tcgtaaacct gttttagttg gcgaagttga
    35341 aaagatgtta aaagaaatac taccatataa agcagatgaa cttggagcag aaataataga
    35401 aatggaaact gataaagaac atgtgcactt attaatatct tgtgaccctc aatatggaat
    35461 tcataaagta gtaaaaggtt taaaaggctt tagttcgaga gtattaagag agaactttcc
    35521 acatttaaag tctagtatgc ctagcatgtg gactaatagc tattttgtag gtactgttgg
    35581 ttctgtgagt ttagaagtag ttaaacaata tatagaaaat caacaagtta ggacaaagaa
    35641 atgataaaaa ctcatagagt aaaattaaac ctaactagaa cacaatttga attagtaagg
    35701 gaaaagcaaa tggaaagtgc taactgttgg aactacatag ttaacttatc taaagaatac
    35761 tattttgaac ataaacaatg gattaaaaag aatgatatac aaaagttaat caaaggtaag
    35821 tacaatcttc atagtcaaac tattcaagct atatcagata aatttgatgc aaatagaaag
    35881 actatatcag aattaagaaa aaaaggtaat actaaagcaa aatatccata caagactaag
    35941 aaattttata taataccttt taaagctagt gctattaata gaaatgctaa aggcaactta
    36001 aaattatcaa tgtcaaaagg caggtattta gaattagatt ttaatgttga gaatataaaa
    36061 actgctgaaa tagtatggcg aaatggatat tatttatact acacttttga caatgaatta
    36121 aatggagttg tagccaaagg cactaatacc gtaggtgtag acttagggga aatacattcg
    36181 atagctagtg taacaaatga aggtgtaggg cttattctat ctaatagaga aggcagaagt
    36241 attaaacaat ttagaaataa gatgtatgct tatatatcta aaagattaag taaatgtaaa
    36301 aaaggtagta gacaatcaaa gaagttatgg agattaaaaa ataagataag aagtaaaaca
    36361 gataatcagt taatgaattt atatcatcaa acaactagaa agtttataga cttttgtgta
    36421 gagcagaagg tatgtgaaat agttcttggg gatataaaag gggttgaaaa agatactaag
    36481 aaaaagaaaa gattaaacag agttaataga cagaaaatat ctcaaatgga gtatggtcga
    36541 ataaaggact atataaaata taaagcaaaa gagcaaggta ttgaagttaa gttagttaaa
    36601 gaaaactaca cttctcaaac ttgcccaaag tgttctaaaa aacacaaacc aactggtaga
    36661 acttatagtt gtacttgtgg ttatgaaact catagagata tagttggtgc ttggaatatt
    36721 ttaaataaaa agcataaata tggtttagta gattttagaa taaatcataa gcaaccaata
    36781 aatttaaaag tatctaccgt ttgagccatg tgtgggcgaa taactttaag gtagtagtgg
    36841 cgaagacacc gtccgaagta gcttggcttt gtaaccacct ctgttgtact gtttaagtat
    36901 aaataagtgt gttagccacc aagaatcccc tt
//
LOCUS       DAEOSP010000017        35908 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.017, whole genome shotgun sequence.
ACCESSION   DAEOSP010000017 DAEOSP010000000
VERSION     DAEOSP010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35908
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.017"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            168..1004
                     /locus_tag="KRH54_000664"
     CDS             168..1004
                     /locus_tag="KRH54_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG8578336.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            1025..1447
                     /locus_tag="KRH54_000665"
     CDS             1025..1447
                     /locus_tag="KRH54_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBG8578337.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(1658..2821)
                     /locus_tag="KRH54_000666"
     CDS             complement(1658..2821)
                     /locus_tag="KRH54_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG8578338.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(2875..3075)
                     /locus_tag="KRH54_000667"
     CDS             complement(2875..3075)
                     /locus_tag="KRH54_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBG8578339.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            complement(3281..4957)
                     /locus_tag="KRH54_000668"
     CDS             complement(3281..4957)
                     /locus_tag="KRH54_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBG8578340.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            5174..5911
                     /locus_tag="KRH54_000669"
     CDS             5174..5911
                     /locus_tag="KRH54_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG8578341.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            complement(6028..6486)
                     /locus_tag="KRH54_000670"
     CDS             complement(6028..6486)
                     /locus_tag="KRH54_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG8578342.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(6768..7055)
                     /locus_tag="KRH54_000671"
     CDS             complement(6768..7055)
                     /locus_tag="KRH54_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBG8578343.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            complement(7133..7558)
                     /gene="ruvX"
                     /locus_tag="KRH54_000672"
     CDS             complement(7133..7558)
                     /gene="ruvX"
                     /locus_tag="KRH54_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBG8578344.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            complement(7764..9935)
                     /locus_tag="KRH54_000673"
     CDS             complement(7764..9935)
                     /locus_tag="KRH54_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578345.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            complement(9945..10202)
                     /locus_tag="KRH54_000674"
     CDS             complement(9945..10202)
                     /locus_tag="KRH54_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBG8578346.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            complement(10227..12866)
                     /gene="alaS"
                     /locus_tag="KRH54_000675"
     CDS             complement(10227..12866)
                     /gene="alaS"
                     /locus_tag="KRH54_000675"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBG8578347.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDRESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     misc_binding    complement(12934..13172)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(13315..14394)
                     /gene="mnmA"
                     /locus_tag="KRH54_000676"
     CDS             complement(13315..14394)
                     /gene="mnmA"
                     /locus_tag="KRH54_000676"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBG8578348.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     gene            complement(14683..15123)
                     /gene="nifU"
                     /locus_tag="KRH54_000677"
     CDS             complement(14683..15123)
                     /gene="nifU"
                     /locus_tag="KRH54_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBG8578349.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            complement(15177..16370)
                     /gene="nifS"
                     /locus_tag="KRH54_000678"
     CDS             complement(15177..16370)
                     /gene="nifS"
                     /locus_tag="KRH54_000678"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBG8578350.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            complement(16388..16834)
                     /locus_tag="KRH54_000679"
     CDS             complement(16388..16834)
                     /locus_tag="KRH54_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBG8578351.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            complement(16943..17713)
                     /locus_tag="KRH54_000680"
     CDS             complement(16943..17713)
                     /locus_tag="KRH54_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8578352.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            complement(17744..19009)
                     /locus_tag="KRH54_000681"
     CDS             complement(17744..19009)
                     /locus_tag="KRH54_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBG8578353.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            complement(19116..19901)
                     /gene="codY"
                     /locus_tag="KRH54_000682"
     CDS             complement(19116..19901)
                     /gene="codY"
                     /locus_tag="KRH54_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBG8578354.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            complement(20066..22153)
                     /gene="topA"
                     /locus_tag="KRH54_000683"
     CDS             complement(20066..22153)
                     /gene="topA"
                     /locus_tag="KRH54_000683"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBG8578355.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNQILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            complement(22186..23286)
                     /gene="dprA"
                     /locus_tag="KRH54_000684"
     CDS             complement(22186..23286)
                     /gene="dprA"
                     /locus_tag="KRH54_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBG8578356.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            complement(23305..24906)
                     /locus_tag="KRH54_000685"
     CDS             complement(23305..24906)
                     /locus_tag="KRH54_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBG8578357.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSDTECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            complement(24937..25281)
                     /locus_tag="KRH54_000686"
     CDS             complement(24937..25281)
                     /locus_tag="KRH54_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBG8578358.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVASYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            25465..26628
                     /locus_tag="KRH54_000687"
     CDS             25465..26628
                     /locus_tag="KRH54_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG8578359.1"
                     /translation="MNREKLVAFIRYLSLFILVVSYLKNVENTLYPVITMIILFLIII
                     NNQVRFFSLSNENTFILISYFLELILIFLLCNYTKTFNSIYFVPLILDISFLIKEKYK
                     YILFSFVIISSLVISLDKNIYLALESSSILLIITVLSIYIENENLSKLYSQNLYDKLR
                     ISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLL
                     KDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIKLTNI
                     NVKLTISKNTWNLSRNQSITLYRLIQESLSNSSRHGKATEIRIFITFNTSNLIITISD
                     NGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            26621..27268
                     /locus_tag="KRH54_000688"
     CDS             26621..27268
                     /locus_tag="KRH54_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8578360.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            27397..28332
                     /locus_tag="KRH54_000689"
     CDS             27397..28332
                     /locus_tag="KRH54_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8578361.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTSFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGN
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            28346..29443
                     /locus_tag="KRH54_000690"
     CDS             28346..29443
                     /locus_tag="KRH54_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8578362.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGDLIFTCLIISTILIALAIVKENYKWREV"
     gene            29446..30510
                     /locus_tag="KRH54_000691"
     CDS             29446..30510
                     /locus_tag="KRH54_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8578363.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            30754..31251
                     /locus_tag="KRH54_000692"
     CDS             30754..31251
                     /locus_tag="KRH54_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG8578364.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNIN"
     gene            complement(31428..31676)
                     /locus_tag="KRH54_000693"
     CDS             complement(31428..31676)
                     /locus_tag="KRH54_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578365.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(31742..32758)
                     /locus_tag="KRH54_000694"
     CDS             complement(31742..32758)
                     /locus_tag="KRH54_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG8578366.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(32816..33583)
                     /locus_tag="KRH54_000695"
     CDS             complement(32816..33583)
                     /locus_tag="KRH54_000695"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBG8578367.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(33651..35840)
                     /locus_tag="KRH54_000696"
     CDS             complement(33651..35840)
                     /locus_tag="KRH54_000696"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBG8578368.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT        11482 a         6233 c         3917 g        14276 t
ORIGIN      
        1 cttttattat tatatcatat tcattgtgaa aatataataa ttacacctac tgtaagtata
       61 atattatatt gacatgaatt gacattattg tctcttagtc aatattatac aagaagtata
      121 aaaaaatatg tgttacactt tgtataacat gaaaggaggt tacctaagtg agacaagaag
      181 aacgcacagt atgctttgat agggaattaa aaattgaagc atatagattt aaaggcataa
      241 tgcaaaaatt cccaaaccat tttcatgaac attatgtaat tggttttata gaaaaaggtc
      301 aaagatattt atcttgtaaa aataaagagt ataccacaag tactggagat ttgctacttt
      361 ttaacccttt tgatagtcat acttgtgaac aaatagatga taaagtactt gattacagat
      421 gtattaatat taagccagaa ataatgaaaa aaaccgtctt tgaaattaca ggtaaaaatt
      481 atttaccaaa atttaatcaa cctgttatat ttcgcagtga gctagtacct ctactacaag
      541 aattacacta tataatcatg gaagaagaat tggattttaa aaaggaagag ctttttttct
      601 tcctaattga acaattaatt gaagaacata cagagccaaa tttgcaatct aacttggaaa
      661 atacaaatat agaaattcaa gcagtatgtg attatctaga aaataattat gctgaacata
      721 tagtacttga cgaattaagc actatagcag gtatgaataa gtactctcta cttcgtaatt
      781 ttacgaaatt aaaaggtatt acaccatata gatacctaga aaatatacgg gtaaacaaag
      841 ctaaaaagct tttagaaaaa ggcgtagaac ctatagatgc tgcgatacaa acaggatttg
      901 tagaccaaag tcactttaca aactttttta agaattttat tggtcttaca ccaaagcagt
      961 atcaaaatat atttataaat gatgattcaa acaactttaa ataaggtaaa aaggagaaat
     1021 aaaaatggaa actaaaaatt caaaatgtgt catggtaata gacgaaaatt taccaatggg
     1081 aattatatcc aatacagcag ctataatggg aattacacta ggaaaacatg ccccagaaac
     1141 tgttggccca gatgtaattg acaaaacagg taatagccac ttaggaatta ttgacattcc
     1201 tgtacctatt ttaaaaggaa acaaagaaat aataaaagat ttacgtaaaa aattatatac
     1261 tttggaattt aacgatttaa ctgttgtaga tttttcagat gtagcacaaa gttgcaatct
     1321 ttatgaagaa tttacacaaa agattgctag tgttccagaa gatgagttac agtattttgg
     1381 gatagcaatt tatgggaata aaaagaaagt gaataaactt actggtagta tgcctttact
     1441 tagataattg ttaaacaacc ctatatatga tattaaatca aatgatattt taagatacat
     1501 aaacttttct atatttctat aaaaatcata agattataaa atacttctat atggtaaata
     1561 aaaatacccc tatggcaaat aattacataa gggtattttt tatgtgtatt ataatcatat
     1621 ctttgtgtat tataattata tttcatctga taattattta tatcatatta tcaattattc
     1681 tttgaagcat ttgtagatag cttgccttat ttatatcctt agtatttatc aattccactt
     1741 cgcctattac ttttttatct ttacatatct ttatttttcc taaaacagtt ccctttttaa
     1801 ttggcatctt aggatttttt actatttcaa ttttcttctc aaattcttta gaacttcctt
     1861 ttttaataag agcatttaaa tcttctttag caaccaattt aactttgttt tcatcagcct
     1921 tatccaatgt aagtgttgct atattatcac ctttactaca taacttaaca ctttcataat
     1981 tagcaaatcc atagtttaaa aggctagagg catcattaaa tctttctgga gaagtctctg
     2041 ctcctaaagt tgcagcaact agatgagtat tacctctctt tgcagatgct gataaacagt
     2101 attttgcttc ttgagtaaaa ccagttttaa caccagttgc tccttgataa tgctttataa
     2161 gtttattagt atttgcaaga cctactgtaa cttgtttctt ccctactaca accttatcca
     2221 tccatgtagt taagtattta cttatcattt cgtgttttaa tagttctcta gacattaaag
     2281 ctatatcatg agcagatgta tagtggtttg caactggaag tccatttgta tttgcaaaat
     2341 gagtatcatt catatttaac tcttttgctt tagcattcat tctatcaaca aatccttcaa
     2401 cacttccagc taagtgttca gccatagcaa cacaagcatc atttgcagaa gcaacagcta
     2461 taccttttaa taatgtatca accttttgag tttctcctgg ctctaggaat atttgactac
     2521 cacccatact agatgcagtt tcacttactt gaacttcatc atcaagctta atcttgcctg
     2581 aatccaaagc ttctactata agtagcatag tcattacctt agttacacta gcaggaggaa
     2641 gctttttatg agcatccttt tcatataaaa tttgtccact accaacatcc atcaaaattg
     2701 ctgattttga agatatattt agttggttgg catcagcatt ttccttatta tttgcaaaag
     2761 aaaagcttaa aggagtaagc gcaattaata tagccaaagc agtactagct aattttttca
     2821 tcgatgtcac ctcttaaagt ttatttaaac ttattttctc acatttaact ttttttattc
     2881 taaatgccta gatatacctt gacctaaatt ttttttcttt tcaagctttt taaagtaacc
     2941 ttctaaaaca cctttattag acgcattttc tattatttca aaagcatctt cataattcat
     3001 tccatactct cctgctattt ccatttttgt ttgttttaaa gcttttatag cattttgtct
     3061 tgaaacatca tccataagaa tcctcctatc agtaaaattt atatataagt agtttttact
     3121 aataaaagat tgttataatt ggtattttat gtaatatata tttttttaaa atgagtatta
     3181 aaaagacctc ttgtaattct taagataata tcaattggat ttttgatatt actttaaaaa
     3241 ttacaagagg tctaattttc aaatttatag tttttttata ttatacttcc attatgatag
     3301 gaagtatcat tggatttcgt ttagtttttt gatataaata ttcttttagg ttttctttaa
     3361 tattattctt taaataagcc cattctttaa tatttttctc ttcacattca tttagaacat
     3421 tcttaattat atcctttgct ccatccatta aatcttctga ctctcttaca tatacgaatc
     3481 ctctggaaat tatatctgga cctgctaaga ccttaccctc atccttggaa atagtaacta
     3541 caactatcat aagaccatct tctgataaat gctttctatc ccttaaaact atattaccta
     3601 catcgcctac tcctagacca tcaactaata tgtttcctgt ttgtatacgg tttgctacct
     3661 tagcagaatt tttatctaat tctaaaacat ctcctgtttg catgacaaaa atattttctt
     3721 tatccatacc aagctgttca gctatttcag catgtctttt taacattcta tattcaccat
     3781 gagctggcat aaaaaacttt ggtctaacta atgcatgtat aagttttaat tcttcttgac
     3841 aggcatgtcc agaaacgtgt atatcagcta tatcactgta tactacttct gctccttttt
     3901 caaataagaa attgattact cttgatatta atttttcatt tcctggtatt ggatgagcag
     3961 atattataac taaatcgcca ctctttattt ctactttttt gtgctctgaa aaagccattc
     4021 tagcaagagc tgacataggt tcaccttgtg aaccagttgt tattatcact aattcactat
     4081 cttcatactt acatatatca tttaaatcta taagcatgtc atcagaaata tctaggtatc
     4141 ctaattcttt tgcaactcca actacattga ccattgagcg acctgatata gcaacttttc
     4201 tattaaactt ttcagcagta tttattattt gttgtaatct gtgtatgttg gatgcaaagg
     4261 tagcaactat aattcttctg ccatttcctt ttgcaaataa ttcgtcaaga cctaccccaa
     4321 cagtcttttc agacatagta aatcctggtt tttctgcatt agtactatct gccagcatta
     4381 aaagaactcc ttttttgctc aactcacaga ttctatgcat atccataaca tctccatcaa
     4441 taggagtcaa atcaatctta aaatctcctg tgtggtatat tataccttgg tctgtgtgta
     4501 ttgcaataga gtaagcatct ggtatactat ggttattttt caaaaactct acttccatat
     4561 tatcaagctt tataacttgt ctaggaccta taacatttaa cttaacatta ttcattttat
     4621 gttctttaag ttttacttgt ataagaccta tactaagtct tgccccatat acaggtacat
     4681 ttattttctt taatatatat ggaagagctc ctatatggtc ttcatgtcca tgtgtaataa
     4741 atataccctt tattttatcc ctatttttta ctaaataagt tatatctggt ataactatat
     4801 ctactcctaa catttcatcc tctggaaaac taagtccagc atctataaca attatttcat
     4861 ctttatactc aacaacagtc atattttttc caacttcgtt aagtccaccc aaggccatta
     4921 cctttatctt gttggtgttc tttttgaaca attgcatcat ctccttctgt ctatttaatt
     4981 ttttaacacg ttctacaatc ataaataata tcaaatcacg cactcgctac cagttattct
     5041 tattgtacca taattcgtat catattttaa gctatattta aaatacattt aacgtattat
     5101 tttgacaaac ctattttatt ttgatatcct ctaaatatta tatgatttaa tatcggataa
     5161 tttaggagga tttatgcgtg gtaaaactca ttgtgcaata ggaatcttaa cagctatcca
     5221 aacatcttta atatttaaaa ttcctatatc tttagttgat attttagttt cagctacttt
     5281 tgctgtacta cccgatttgg ataaatctaa ttctatggtc tcaaatttca ttctaaaaaa
     5341 caatgtatcc aaatacatat atagaatttt tatatatgct gttaatatta ttattttctt
     5401 tatttcaata aatataaatg ataattttta tttaagcgca ataattacat ttgttgcaat
     5461 tataattatt gaagcaaaac taacccacac atttctaaga aaagtttttc tatcattaat
     5521 atttatacta ttagctatat gcttatacat catagaagtt gaaatatatt ttacaatttt
     5581 ttgtctaatg ttgtcaattt ttccctggtt aaagcacaga agtttttctc atagcatatt
     5641 tgcaactata atagtatatt ttttactaaa acagatagaa ttaatcacta atattaataa
     5701 tttatctttt tatggtacga ttggttatgc aagtcatatg tttttaggag atttatttac
     5761 aaagcaagga ataccaattt tttatccgct aagcgaaaag aaaatttctt taggtttttt
     5821 aactgttgga ggaccattta gtaatttcat agaaaaatca ttcatttttg tacttatagg
     5881 attaattata ttttcaatat taaaactata agcatcttga aaactgtata tttgcatttt
     5941 gtagatggtt tataataaaa aatggaagaa tagcatagaa tataaaagtt ctatactatt
     6001 cttccatcga cattaagcaa tagaagttta ttcttctgaa tcgcttactc cattacactc
     6061 gtcacatagt ccataaaatt taacatcatg atcaagtatt ttaaatttgt acaatgattg
     6121 tatctctttt tctatattgt ctaaaaggtc ttctttagct tccattatct ttccacaatt
     6181 tttacaaata agatgatgat gattatgaca gtcttcttta tgtagactaa tctcatatct
     6241 tatacatcca tcgtctaagt ttagttttga tattaatcct atttcatcca atagctgcat
     6301 tgttctatat acagtagcta agcctatttc aggacagtca accctaacta aatcatagat
     6361 ttcctcacta cttagatgtg aatggtcatg tttaagcaat atttcaacta ttgctcttct
     6421 ttgtggcgta atcttaaaac cagtttcttt taacttgtct tttaataaat ccattgtatt
     6481 tgccataaca tcacccttac tattttcaat tgataatctt tattaaataa tattgtaatt
     6541 gataattcat tttttgtcaa gacatttaaa taaatttaat ttattctcat aatataaata
     6601 tatacccata aaagatatat tattcaccaa aattcataaa ttttttggta tttttaataa
     6661 aaaacaaaaa aacagatata tactataaat gcttttacaa ttaataaggt ttaattaaag
     6721 caaatataat aaatatctga tttttatata caaacttaat tacttatcta gttaagtcca
     6781 tttgattcat cttcgtcgta atttaaacct tcttcttcca ttatagcagt atacacagct
     6841 acaacagttt catactcttc atcactttcc aaaggtatta agatttcacc ttcttcatcc
     6901 tcatcaattc taaagattaa agcttcttca gcttcttcat catctaaagg cattaatatt
     6961 gcatactctt ttccttctgc ttctaatgtt aatattattt caaattggct ttcaacgcca
     7021 ttttcgtcta ttaaatttat tatattttct tccataatgt cattcacctc aaatttattt
     7081 ttatttttat tatttatatt atatatcata agtattcaat tttatagttc ttttatgaat
     7141 ttattttaaa atctaaatat ccttgcaaaa ttattactgc tgccaacata tctataacct
     7201 tttttctttt ttgtctacta acatctgctt ctaataaaga cctctctgcc gctactgtag
     7261 aaagcctctc atcccaaaac tctacatcaa tacctgtttc tgcttttacc aattcacaaa
     7321 acttgattac tttttctcct tgtggaccta aagttccatt catatttttt ggtaatcctg
     7381 atacaatttt atttacttgt ttttctttta ttattgcttt tagctcttca atatcttttt
     7441 tcttgccaac tctttttatt gtttttacac cttgtgcagt taatcccatc aaatcactca
     7501 cagcaactcc aatagtttta tcaccaacat caagacccat tattcttcca tctaacatag
     7561 ttcctccttt atctatttat attctataga ttaaatacat attctgaacc tctcataact
     7621 aaggtcacag gcttactgct tcatataatt ttacataatc aatcataaaa attgcaatat
     7681 gattttttat gtaagcatcc agtttacatt aataatcata ttgcattttt tatttaaatc
     7741 ctatatgaac agtaaaaatt tttttagtta tttttttcaa atattatcat atttatactt
     7801 attattatta gtggtataag tatatatata gatgctgtag atgcagcaac tattccagaa
     7861 tcattaacta aaagagtaat tatacaacca accatagatg caataaaccc tttaaacaag
     7921 attggaaaat tattcattat ctttctaaag tgtctagatg gtttaaatat aagcactgca
     7981 attactccaa ttcctacaag taaaatattt acccatacac ttgtctgtgc tagttgcata
     8041 ttcatttgaa tttttcttcc aaatgtttgt aatatctctc ctggtccatt taggaaaatt
     8101 ctctgtacaa acatacctaa atgagattca ctacctgaaa gtatatctaa agctgcaaat
     8161 gcaaaaacga caactacagc tgaaacagct aataatacag ctttcttaaa atctatttta
     8221 atgtcatata taagcattat aaacaacaaa tatgctacac attctgatat agctccacca
     8281 acatttgcac ccattgaagg atatgcacta gttataagta ttattaatga aaaaattaca
     8341 acaagtaatt tagatacctt cttataatgt aataatactg ctaagccaaa tactgcactt
     8401 gctatagtta caccttcata ttcatttcct acaccatagt acctagctcc tatgacagca
     8461 tcataactca ttatgttatt ttgcattaag tatgttccaa atacagaatc tataactatg
     8521 actaaaatag taagtgttgc aaaaaatccc atgtgcttta tatcattgtc cttaaataat
     8581 tttctaccta acaagtataa tattagcgta gtcgcaaata ttcccaaaga tattgaaatt
     8641 ggagttttaa aattcatcaa aggagctaac ataaatgata gtggcattat tatgcctaat
     8701 tttataaact ccttaagtac tttgaataca cgctctttat gtggcatata atttctaaag
     8761 aaaatcgcaa acatggcaat tacccaagaa gcagatatta cgcctacaaa gccatttata
     8821 atagttgacc ttattgaaga tatagacact atcctttcat aaagtgattt tagatagcta
     8881 atattgtcct ctttatttac taaagtataa cttctaccta ccatctcttt attttcaaga
     8941 ccaaatgtac ctagtatatc tactccaaca tctaaattgg caacaattcc agcctttctt
     9001 gttgtggcag attccagtag acctttccct tctccactaa attttattat tggagaaagt
     9061 cttctcttat tcttataatc taggtcactt ggaaatgcac ttgctatata aacagtgtca
     9121 ttttctccaa ccatagaaaa tacttgttca agatatttat ttatattttt tacaatattt
     9181 ttcttcattg tatcataagt attatcattt agatttaatc tatacatatc taatctatat
     9241 gtatctccaa gttctacaaa tatagcatca ctatttttat aggatttttt agtttcatct
     9301 agtaatttat cataatctgt agatattcca aatggcattt tagaatcttt cttattaata
     9361 ttatcaatat tacctgaatc aatccttcca tattggtcca tagctactaa acataagttt
     9421 ctgtttttaa ctaattgatt attttcaatt atatcagcat ttccaattac agatgtcttt
     9481 aaaccatttt ctgataaagt ttttcctaat gaccctaaat ttgctccata ttctccatac
     9541 tcaagatttt catttataga tctattaatg cctaaatcat ttatttcttt agctttttga
     9601 cctgttgcga cttcaaaagc tttagcagta tcttggtcta ctttttgaaa attgatatag
     9661 tcttcagtag ttacactagc tcttgttcct gcacccatac ttgcataaga ccttctatca
     9721 tcagtacctt gatcccctct tatattcatg agagctacat agcctctctt ttctatctca
     9781 tttttcaaga ttggaatatt catcatactt ccaagattgg tcctattcat atcaataaat
     9841 atgaccttgc ctttttcttc agcaaaacca tagctagcag ttgatacaaa aatcataatg
     9901 actattgcaa acaacgacat aatctttttt ctcatttatt tgacctactt tctattcaga
     9961 tagtgcgtga ttacttcttc aagtatctca tctctttcaa attttttaat tatagctctt
    10021 gcatttttat gactagttat atagctagaa tcaccagaaa ggagataccc tataatttga
    10081 tttatagggt tatatccttt ttcaactaat gctttatata caaaatcaat agtatctcct
    10141 acactcatcc tatcttctgg tattccttca aatttcatag tgtagtctaa atctttttcc
    10201 aaactaaaaa cctccttgag tgaatgttat ttaacttgtg tcttaataac ctcacttgca
    10261 tagcttaaag cttggtcaac cttagataca tcactagctc ctgcttgtgc catattaggt
    10321 cttcctccac cttttcctcc agctattttt gcaacttctc taacaatatt acctgaatgt
    10381 attcccttat ctaatacatc tttagtggca gtaactacaa agttaacttt atcatctact
    10441 acatttgcaa gtactactac accagaacca agcttatctc ttagattatc tgctgtttct
    10501 cttagtgtat ccatatccat accttcaaat ttattagtta tcaaattaac tccatttact
    10561 tcaactttag aatcaaaaat agaatcaact gattgtaaac tcatcttagc tttcatatca
    10621 tgcaattctt tagataagtt tttattttct tctaataaag aacttattct ttgaattaaa
    10681 ttatcttctt tagattttaa gtttacacaa acttcagaga taattccatc tctttctttt
    10741 aggtattcat aaacagcttt tcctgttatt gcttctattc ttcttacacc tgctgctact
    10801 ccaccttcag ataatatttt gaacattcct acttgagaag tatttgtcaa gtgagttcct
    10861 ccacaaagtt ctgtagaata atctcccata gaaactactc ttacctcatc accatatttt
    10921 tcaccaaata gtgctgtagc tcctttttct ttagcttcct ttatattcat aatatcacat
    10981 tttatgtcta aagatgataa aataacatta tttacttttt cttctattac ttttaattct
    11041 tcatttgata tcgcttcaaa gtgagtaata tcaaatctaa gtctttctgg agttactaaa
    11101 gaaccagctt gattaacatg ttctcctaat acttctctta atgccttatg aagtaaatgt
    11161 gtagcactgt gatttcttgc agctgacatt ctagtttctc tatctacgag tgtctttact
    11221 ttgtccccat tacttattct tcctgatttt attataccta tatgttttat actattatta
    11281 gcaccttttt tagtatttaa tacttctaaa actaagtttt cattagttat caatccacaa
    11341 tctccaactt gtcctccacc ttcaggatag aaagtagtgt tatctagtac tatagatacc
    11401 ttatcgcctt cttctgcact ttgaactagc tcatcatctt taactatagc ttcaatagtt
    11461 ccttcaccat aaatttcact atacccatta aatgtactgt caactgtaga ttctaatttt
    11521 gatagagggt cttccttcca actttctcta tccatatttc ctctagcatt tctagctctt
    11581 tctttttgct tttccatttc ttcattaaaa gcttcttcat ctacagatag atgttcttct
    11641 tctaatattt ctttagttaa gtctataggg aagccataag tatcatatag tttaaaagct
    11701 tcttgaccac ttaggacagt ctttccttca ttttttaata cttctatata agagttcaat
    11761 atttctgttc cttggtctat agtttcattg aatttttctt cttctattcc aactactttt
    11821 ttaatataac tttctttttc aactaaaact ggatatgctt tatcatttac ttttataact
    11881 tcatctatta gtttctgtaa aaataattct tttactccta ataacttacc atgacgagca
    11941 gcacgtctaa ggagtcttct aagaacatat cctcttcctt catttgatgg aagcactcca
    12001 tcactaacta agaatgttac agctcttatg tggtcagtta ttattcttat tgatatatca
    12061 ttttttggat tctctccata ttttactcct gttaacttct caactgcttc tagtatagat
    12121 ttaattgtat caacttcaaa gatagtgtct acgccttgca taatacatcc cattctttca
    12181 agacccatac ctgtatctat attcttgttt tctaatagac tataatttcc ttcttcatct
    12241 ctatcaaatt gagtaaatac atggttccaa aattctaaat acctatcaca atcacagcct
    12301 ggtttacagt ctggattgtc acatccatat tcttctcctc tatcaaaata tatttctgaa
    12361 caaggaccac aaggacctgt acctatttcc cagaaattat catcttttcc aagtctaacc
    12421 attctttctt ctgggaaatt catttctttt gcccatatat cataagaatc atcatcttct
    12481 tcatatacag tcacccatat cttatcttct ggtatattta gccattctgt aacaaattcc
    12541 catccccatt tgattgactc tcttttaaag taatctccaa atgagaagtt acccatcatt
    12601 tcaaagaatg tagcatgtct tgcagttata cctacatttt ctatatctcc tgttcttata
    12661 catttttgag atgtacacat tctcacactt ggaggtactt ctacacctga aaagtagttt
    12721 ttcaaaggtg ccatacctga gtttataagt aacaaactct tatcattgtt tggtactaaa
    12781 gaataactat ttgctacata atgacctttt gactcaaaaa attctaaaaa cttacttctt
    12841 atttcattta atcccatctt ttccatatca tgttcctcct aataaatttt attttttcgt
    12901 ttaaataaaa aacctcgtcc ctataaataa atttataggg gcgaggacaa tcgcggtacc
    12961 accctaatta tcatacaaag tatgatatct tcggaaaacc ttagctataa cgtaactacc
    13021 gtcaatctct acttaaatct tctttataaa atataaattg ttcaagattg ctactaaaag
    13081 gctgcttcaa atatcatatt ctaagggctt ccagcatttc cctctctctg taagacatca
    13141 aatattttac tattccttat cactgtattt gttttttaat taatatacta attatattac
    13201 taaattttac aaaattaatc aatactatat tttcatatac aataaattta ttttataaac
    13261 tttatttact cttaatatac tttattgctt atttttttaa ttttttagat gtttttataa
    13321 acttttctcg attatacctc cacctactac aatatctcca tcatacatta ctacagattg
    13381 acctttagta atagctcttt gagcttcatc aaaaactatt ttaatagtat cttctcctac
    13441 tctatgaata gtagctggtg atggttttgc tgaatatctt attttggctt gaactcttaa
    13501 gggttcttct aacgtatcta ttgatataaa gttaacatct ttagcaataa gttccttttt
    13561 aaataaatct tcattgtctc caagaactat ggtattattt attggattta tgtctattac
    13621 aaacattggt tttccaaaag taattccaag cccttttctt tggcctatag tataatataa
    13681 tataccctta tgttttccaa gtatattgcc tttagtatct acaaaaaaac cttcttcaat
    13741 acgcttttta gaatgttttt ttacataatt tgcatagtca ttatctttaa caaaacaaat
    13801 ttcttgacta tcaggcttat tgtgtactgc catacccatt tccttagcaa tttctcttac
    13861 tctatctttt tcatagtcac ctattggtag taaagtatgc tctagttgct cttgtgtaag
    13921 attataaagt gcataagttt ggtctttttt atctgtaaca gattttttta gcaaatatct
    13981 tcctgtactt tcatcctttt ctatctttgc atagtgacct gtagctacat aatcacaacc
    14041 tatttgtcta gccttcttat aaaaatcatc aaattttata tgtttattac aagctataca
    14101 gggattagga gtttttcctt ccaaatattc atctataaaa taatctatta ctttttcttt
    14161 aaacacttct ctaaaattta aaacataaaa tggtatacct attttatttg ctacccttct
    14221 agcatcttca acagcagata aagaacaaca accaccttca atttcaacaa catcatcatc
    14281 ttgccacaac ttcatagtaa caccaatgac atcatatcct tgttgtttta aaagatatgc
    14341 agctacagag ctatcaactc ctccactcat acctatcatt acttttttat tcataaacaa
    14401 atttcacctc atcttttgca ttacttatta ttataccata tagagcaagt attgtactta
    14461 tttaaaatat agcttgttct attttctaaa aaaataatca tctttattat ttgaatctta
    14521 tggattattt tgttaactaa ttaacctcta aatcaagtta aaatacttaa tttaggcaat
    14581 aaaaaatagc cattttaaaa acagctattt tttatgtcct aattatatca ttagtacttc
    14641 tttttaatca ttgtaaactt ctcataacac aatcacttat ttttattctt cttcttcaac
    14701 atcatggtca tgagcatcat ctataacata tccatccaat tctggtatat ggatattgtt
    14761 cttttgagca taatctataa gagctgcttt tacagcttgt tcagctaaaa ctgaacaatg
    14821 catctttact ggtggtaaac catccaaagc ttctgcaaca gctttatttg taagttctaa
    14881 tgcatctttt atagtttttc cttttatcat ttcagttgcc atagaagaac ttgctatagc
    14941 tgaaccacag ccaaatgttt taaacttaac atcctttatt acatctccat ctatgtctaa
    15001 aaatatcttc attatatctc cacatgtagg gtttccaact tcacctacac cacttgcatt
    15061 atcaatttct cccatatttc ttggattcat aaaatgttcc ataactttat cactatattg
    15121 cattatcttt tcctcctact caatttatgt ctatttttta tgctttatct ttatgtctat
    15181 tttactaaac cttgtttctt cgcatcatca taaagtggtg acatgcttct aagtctttct
    15241 attacttttg gtaagttctc tagtatataa tctatatcat catctgttgt aaaatctcca
    15301 actgtaagtc ttaatgaacc atgtgctatt tcatgtggta aacctattgc aagaagtaca
    15361 tgtgaagggt ctaatgaacc agaagtacat gcagaaccac ttgaaccagc aattcctaac
    15421 atatctaata ataaaagtat tccttcacct tctataaatt ggaatgcaaa gtttgcattt
    15481 cctggcaatc tattttctaa gctaccattt actcttgtgt aaggtattct ttctaacaca
    15541 ccatctatta acttatttct aagacgagtt aaagtttcta catgattttg catattttcc
    15601 ttagctaatt ctgctgcttt tccatatcca actattgcag gtatattttc tgtaccagct
    15661 cttttgcttt tttcttgtgc tccaccatgg acaaagttat gaagtcttac accttttctt
    15721 atgtataaag ctcctacacc ttttggtcca tatattttat gtgaagacat actcatcaaa
    15781 tctattccta attctttaac atctactgga atattacctg tagcttgtac tgcatcagtg
    15841 tggaataata tcttatgttt tttagctatt tcggatattt ctttaatagg ctgtatagtt
    15901 cctatttcat tatttgcaaa cattatactt ataagtatag tagtatcttt tattgaatct
    15961 tctagttgct ttaagtctac ttttccttcg ctatcaacat ctaaataagt tatttcaaat
    16021 ccatgatgtt ttgctaagta ttcacatgta tgaagtattg catggtgttc tatttttgaa
    16081 gttattatat gattaccttt gtttttatta gcataagcta caccctctag tgtccagtta
    16141 tcactttctg aaccacctgc tgtaaaatat atttcactag gttcagcatt tataagggct
    16201 gctacttgtt ctcttgcttt atccaaagca tcttttgctt ctcttccgaa tgtgtgaaaa
    16261 ctagatgcat taccaaaata atctgttagg tatggcatca ttgcatccaa tacttctttt
    16321 ttaataggtg ttgttgcaga ataatccata tacaatcttc ttttttccat tacattcatc
    16381 tccaaatcta ttttttaata tttgttatat cattttctaa tttatttttg ttatagtcat
    16441 ttatcatatc tttaagagta attgaatcta taacatcttc tatacccttt ttcatctttt
    16501 cccaaactat ttttgttaca cacatgtttg agttttcaca tacatcttca tctagtacac
    16561 agtcagataa tgcaactggt ccttctaata ctacaagtat atctcctact gttatgtctt
    16621 cagcattctt tgaaagcaaa taacctcctt gagcacctct cacacttttt actagacccg
    16681 atttttttaa agatgaaaat atttgttcta agtactgatc tgatattttt tgcttcttag
    16741 caataaattt taatgatact ggtccattgt cttgatttaa tgctaattca aacattgctt
    16801 ttagtccata tctaccttta gtagacaact tcatatatta tcacctctat atagaaatct
    16861 aattcatact gttttagtag gaattacttt caagataatt ttattatacc ctactaattt
    16921 tgtcaagatt aattttaatt ttttattttg aataaattcc ccattttcca atgttttcaa
    16981 atcctagttt tttatatatc tttccagcct cttcattatc ataaaacaaa caaggaattt
    17041 tgttttctct cagtaactct gaacatagtt taattaggca ttttgtagca agtcctttag
    17101 ctctatattt aggatgtgtt cctactccaa ttatcattgc atgagtccta ttttctgaag
    17161 tactctttgc cattgacacc acctgtttgt taatctctat acaatatcct ctacctgtcc
    17221 ttagtccatt ttttaaattc tctaaagttg tactgtgaaa ttcatctatt aagtcataaa
    17281 gttttactgt cttttttaga tttccaagcc taattctctt aacctttgca ttacaattat
    17341 tatctatcaa aaacttttta gtttgaagtt tgcaaaaatc aactaccttt aaattattaa
    17401 gtcctaatct ctttgctaaa gcatctacag cacatatttt cccagatatt tcttcgtaat
    17461 tagtattacg aagtagatta taaaaacctt caacatcata ctctccatca gaataaaaca
    17521 tcataaattc aaaatattta agtaatattg cctttatttc accatgttca cccacatctg
    17581 cccatatatt taaaaaatta tttccatatc catatctttc aatatcccct attataaaca
    17641 aattgtactc aggttctttt tttaagtatt tcataactct actgtgataa gttgagttaa
    17701 gttttataat cataatatct acccccgata cataaagttc agtctaaaaa tcactattta
    17761 gatattctaa atattttttt atatttttct cagttttatt ttcttgtggt atgtaataaa
    17821 tactatcttt tatgctatct ggtaaatact gttgctttac ataatgattt ttaaaattat
    17881 gaggatattt gtatccacaa cccctaccta tttctttaga accagaatag tgagcatcct
    17941 ttatactatt tggtatatcc ttaacaaact ctttatccag ttcatccata gctttcatta
    18001 ttgctataca tgcagaatta gactttggag atgtagccaa aagaatagta gcttctgcta
    18061 atggtatttt cgcttctgga aatccaagtt gcatagctga gtctacacat gcctttacaa
    18121 ttacaatagc atttggataa gctaaaccta tatcttcact agctattact aaaagtctcc
    18181 tacatatact tatcaaatcc ccacctttta taagtcttgc tagatagtgt atagatgcct
    18241 gtgggtcact tcctcttata gatttttgaa atgcacttaa aatatcataa tgactatcac
    18301 catttttgtc atagtttata attttattaa atgtagatgc ccttattaca tctgaatcta
    18361 tataagttat attatcctta tttggtttag ttgaatagac taccacttct aaaatattta
    18421 aggctcttct catatctcca tcagaaagaa ttgctatatc ttcaatagca ttattattgc
    18481 actcaatatc catatagcta tcttcattta atacttcaac agctcttttc agtccttttt
    18541 ctatatcaag tttttctaaa ggtttaaatt caaatacagt agacctactt aaaagagctt
    18601 tatatacata atgatatgga ttttctgtag tgctagcaat caaagttata ctcccatttt
    18661 ccataaattc taatattgat tgttgctgtt ttttattaaa actttgtatt tcatctatat
    18721 ataataggac tccatttaca ttatttatac ttccaagctc tgatatcacc ttttttatat
    18781 cttctaaaga tgaattagta gcatttattt tatagaagct tttattgctt ctttctgcta
    18841 ttatttccgc tactgtagtt ttaccaacac caggaggacc aaaaaatatc atatttggta
    18901 aaaaattaga ttgtaatatc ttagacaata tttttccatt acctattata tgactttgac
    18961 ctattacatc attcattgtt tttggtctta ttttatctgc taaaggcatt ttctcactcc
    19021 tttttaatat acttatcatt accttaataa ttcgtctaaa aaaggtatct atttgaaata
    19081 gatacctttt ctaatctaaa cctataaaat atagattatt gattgttttt taattttttt
    19141 aattcatctg taagtttgtc attaagtatt cttatatgag tacctttcat acctaatgat
    19201 cttgattcta tcacacctgc actctcaaat tttctaagtg catttactat tacagaccta
    19261 gttataccaa ctctatcagc tatcttactt gctacaagta gaccttcttt tccatccaat
    19321 tcagcaaaaa tatgttcaac tgcttcaagc tcggagtagg acagagtgcc tattgccatt
    19381 tgaactacag ctttctttct catttcttct tctaattctt cacctattgc tcttaatatt
    19441 tcaagaccaa caacagttgc actgtattca gctattacta aatcatcatc attgaatgaa
    19501 tttgaatatc ttgaaagtac caatgttcct aatctttgac cgcttcctaa tattggaact
    19561 actgtagtat atttttgtaa tcttccatgt tcttcaggaa agatttctaa tatcttctca
    19621 ccatttaaat tttctaatgt ttcatcaatt tttaacacat tttgagtata ttcatctgaa
    19681 aatttctttt gcttagtata ctcatcttct ataactgaac tgtcttgaac atcatttaaa
    19741 tgaagaccta gtactttacc ttttgcactt actacataaa cattagaact taaaacgtcg
    19801 cccaatgctc cggccagtaa atcaaaagag acactgcttc caccacttgt ttgtaatgtt
    19861 ttatttattt tccttgtttt ttgtaacact tcacttgcca tttaatcatc tcctcatgaa
    19921 atttaggcct tttaataacc ttttatattt tctaatatta ttttcacatt taatgaaaat
    19981 ttatttttct attagttgtt tacacttgaa agattatcat agattggaac ctttttcaag
    20041 agaattatgt gatttttttc aattttcact cttttccgtc attatgctct tttgtctctt
    20101 tattacattc tttattagaa cacactgttt ttgtttcaga tttagtcact ttttcaacca
    20161 tatatgaacc acacttctca caaaactctc ctgttggctt attccaagct acaaagtcac
    20221 agtttggata attagaacac ccataaaaag ctttacctct tttagatttt ctaagtataa
    20281 tatctccttc ttcacatttt ggacatttaa caccaatttt attcataatt ggtctagttg
    20341 ttttacaatc gggatagttt ttacaagcca taaactttcc aaatctacca tatttgataa
    20401 ccatattaga cccacagttc tcgcaaatct catcagtttc ttcatccata tttacttttt
    20461 ctatattttc aagagcttct tcaatagcct ctttaagtgg tgcatatgat tctgatacaa
    20521 tctttttcca gtctgtattg ccttcttcta taacatcaag tttactttcc atatcagcag
    20581 taaaatctac atctataaat ttatggaaat tattttccaa aatatcagta actattattc
    20641 caagttctgt tggacataga cttgttcctt gcttttcaac atactctcta ttcagtatag
    20701 tagctattgt tggagcatac gtacttggtc tacctatacc tagttcttct agtgttttta
    20761 ctaaactagc ttctgtatat cttgctggtg gttgtgtaaa atgttggtct ggcaatatat
    20821 tttctatatt taatatatct cctttttcta tacttggtaa aattttgtct tctctttctg
    20881 aaaaattata taccttggta taaccatcaa atttcatttt tgaaccagtt gccttaaata
    20941 caatatcgcc tattttacat tctacattta gtatatcaaa cactgaatct tccatttgac
    21001 tagctacaaa tcttctccaa attaaattgt ataacttgta ctggtctttg ctcaatgagg
    21061 cttttatact ctcaggagtt ctatcaactg aggttggtct tattgcctca tgggcatctt
    21121 gaaccttttt agtatccttt ttttcttttt tatcaccagt gtttttatag tagttttctc
    21181 ctaaattgtt tacgatatag cccttagctt tttcttttgc ttcatcagat attcttttag
    21241 agtcagttct tatatatgat ataagaccaa cagtaccttc accttctatg tcgatacctt
    21301 catataattc ttgtgctatc atcattgttt ttttagtagt aaaaaataac ttatttgctc
    21361 cttcttgttg aagcatactt gttgtaaatg gttttggagc tgatttacgt cttgatttag
    21421 attctatctt ctcaaccttt aaatctttac cttccatttc ttttagtatt tgatttacag
    21481 cttcttcatt ttctaattca atcttttcat catttttacc ataaaatttc aaagttatat
    21541 ctttgtcttt tacggtctta gcctcaacat ctattgtcca gtattctttt ggaataaatg
    21601 cctttatttc tttttctctg tcacaaatta actttgttgt aacagattgc actctacctg
    21661 cactaagacc ttttctaacc ttttgccata atataggact tatttgataa cctaaaagtc
    21721 tgtctagcac tcttcttgcc tgttgtgcat caaccaaatt tatatcaata tttctaggat
    21781 gtttaattgc ttttttaatt gcatccttag ttatctcatt aaactctatt ctacaatcct
    21841 cagattcatc taaatttagt atatgagcta aatgccatga tatagcttca ccttctctat
    21901 cagggtcagt tgctagatat acttgtttag attttttagc ttcttttttt aattctttta
    21961 ttacatcgcc ttttcctctt atatttatat attgaggttc aaaattattt tctatatcta
    22021 cacctaattt acttttaggt aaatctctga catgaccaac tgatgcttta actgtgtaat
    22081 gacttttccc taaaaatttt tctatagttt tagcttttgc aggcgattct acgatgacta
    22141 atgttttggc cataaaacca tacaccccca ctacctaatc ttaatttata catttaaact
    22201 gtacgtttta ttattcattt ctactactaa ctcatttagt actaattcat tgattattga
    22261 atttacatac tttatttcta taccagtata atcacaaata ctatctatat gcaagtttcc
    22321 ttctcttttt atagcttcaa ttatttgctt agacttggca tttagactta tattatcata
    22381 tttttgattt atttttttat caataatatt aaatatgtta tattcattta aaatatcatc
    22441 tatattttca attaattttg caccttcttt tataatttta tgacaacctc tactcatttg
    22501 agaatttata ttaccaggta ttgcaaaaac attttttcca tgttcaagag caaaatctac
    22561 tgtgattagt gcaccacttt ttattgctgc ttctacaaca actactccat cacttaaacc
    22621 actgataatt ctatttctat tagaaaaatg gtacggggct actgtcgagt taatattata
    22681 ctctgataat aataatcccc cattttctaa tattttattt gataaatgta aattttgttt
    22741 tggaagagga ttgtctactc ctgaaccaag tactgctata gttttagatt ttccactcat
    22801 acaacctatg tgcgagtatg catctattcc tatagctaat ccacttacta tattaactcc
    22861 atattgggat agttgacaac ttattgtttt tgcacaatta attccatagg ctgtaggttt
    22921 tcttgaacct actatagcta tattaaaatt attattaact aagcctatgt cacctttgta
    22981 gaataataac tttggagcgt tgtatatatt ctttaaattt tcaggatatt ctttatcata
    23041 tttacaaata tatttaatcg cttttttgta aagtagttct tttatatttt ctaaataagc
    23101 gtgacctcta tattttacta tattttttct aatattcaag cttatatttt ttaaattata
    23161 tatttctttt tcagaaaaat caaatatatc ttcaatattt actatttcat tttctattat
    23221 ttcaattgtt tttgttgtta ttccaccaat tgattttaac cataaataag tatctctttt
    23281 ttccataaaa tccacctcaa aaaactattt aaaataacta taataagcct ttctaaatga
    23341 aaaagcttct ataatacact gtgaatttat caagtctctt tcttctaaat ctgctattgt
    23401 tcttgccatt tttaaaagtt ttgtataact tctactgctt aggttgtatt tattaaatat
    23461 catctcagcc gtttttgaag cttctttttc cagtttacaa tatttaaata aattgtaagc
    23521 tttaatttca ctattatttt ttatattatc cttcttaaat ctatttatct gtatttttct
    23581 agcattttct actcttctct ttattttttg agagctttct tcttgtttta aagaattaaa
    23641 atctgaatat tttatagaat taacttctac aaaaatatca aacctatcta acaatggacc
    23701 tgatatttta tttatgtatc tatcaatttc attacttcta catctacact ctgtgtctga
    23761 catataataa ccacatggac atggattcat agctgcaact aacagtactc tacaaggata
    23821 ttttgcactg tactttactc tggatatatt tacatatcca tcttctatag gttgtcttaa
    23881 agtctcaagt atttttctat caaattcggc tatctcatct aaaaataaga tacctctatg
    23941 tgctaaagca atttctccag gtcttgcatc cattcctcct ccaattaaag attgctttgt
    24001 agttgtatga tgaggtgctc taaaaggtct tttatcaatt attcctttag tttcatttat
    24061 catacccaat atactataga ccttacttac ttctatcatt tcttctacac ttatatctgg
    24121 taaaattgtc cttactcttt ttgcaatcat agtttttcct gagcctggag gtccaatcat
    24181 aaacatatta tgatttccag cagcagcaat ttctgcactc ctctttacaa aataatttcc
    24241 ttttacatct ttaaagtctt catcatattt actttcttca ttattaatct tagtatattt
    24301 gcaatctata taactattat ctttttctaa ttcttcgttc tcttttcttg acttatcatc
    24361 caaaaaagac attatcctta ctttatcgac ttttatctct tcgtttaaaa aatcgacaca
    24421 ttcttttaaa cttttaatag gaattatatc aatttcatct acaaatagac tttcttttat
    24481 gttctcaatt ggtataaaaa tacgttttat gttctgagtt tttgcaccca taattatggg
    24541 caatatacct cttacttttc ttattttccc atctaatgat aattctccta taaacatact
    24601 ttctcttatg taattttcat ctttttttat taactctctt aaaaggccta tacttataga
    24661 taaatctaaa aaagctcctt ctttttttat atctgctggt gataaattta caactattct
    24721 cgtgcttgga aatttatatc cactattaag aattgctgac tttactcttt ctcttgcttc
    24781 tttaatttct ttgcctggaa gaccaacaat attaaaacta ggtattccat tgcttacatc
    24841 tacttcaacc ttaactaaaa agctgtcgat tcctactaag ttgcttgaat taataatact
    24901 taacatattt aatcctttcc aaaaacatta tcttttttaa aaagcattca ttatgtgatt
    24961 tattcttttt tctaataaat atacctctat aacatcaaat cttatgggag tgttatttaa
    25021 gttatttttt ataatataat aacttgcaac tccaattatc ttttttcttt ttttgaagct
    25081 tacagattca caagggtagc caaactttat gttagacctt gatttcacct ccacaaatat
    25141 aatttcatct tccatttttg ctattatatc tatttcccct atctttaatc tgtaattttt
    25201 ttctagtatc tttgctcctt ttgatagcaa ataattaact gctacttttt ctccaaaatc
    25261 acctttttct ttattattca ttattcactc caatacatta aattttcact tttaaattat
    25321 ggtcagtata caattttact attcaagttt ttggattgat tttatttcca tcaatattaa
    25381 taaatacttc tattgacttt attcttttat gatattattc tagttttcat aggaaaagtt
    25441 tatttttttg gaggagtgcc tttaatgaac agagaaaaat tagttgcttt tatacgatat
    25501 ttatctttat ttatattagt tgtaagctat ttgaaaaatg ttgaaaatac attgtatccg
    25561 gtcataacta tgataatatt atttctaatt ataataaata atcaagttag atttttttct
    25621 ttatcaaatg agaatacatt tatacttatt tcttactttt tagaacttat attaattttt
    25681 ttactttgta attatacaaa aacttttaat tcgatttact ttgttccttt aatattagat
    25741 atatcatttt taataaagga aaaatataag tacatattgt tctcttttgt tataatatct
    25801 tctcttgtta ttagtttaga taaaaacata tatttggcac tggagtcaag ctctatattg
    25861 ctaattataa ctgtactttc tatatatata gaaaatgaga atttatcaaa attatacagt
    25921 caaaatcttt atgataagct tagaatttct aaagatgaat taaaaaaagt taatgctgat
    25981 ttggaaactt atgcaagttc tatagaagaa ttagcaattt taaaagaaag aaatcgaata
    26041 tcaagagaaa tacatgatag tgtaggacat tctctttcta caacaataat acaattaaat
    26101 gctatagaaa aactcttaaa agacaaacct ctaatttatg acttagtaca tgaacttaga
    26161 gaatttgtaa ctgaaagttt tcaagatgta agaagggcta tatctgagtt aaaacctgtt
    26221 gaatatgaaa attaccaaag tttatttaaa atcaaagaat tagttaaaag ctttattaag
    26281 cttacaaata ttaatgttaa attaactatt tcaaaaaata cttggaactt atccagaaat
    26341 caatctataa ctttatatag acttatacag gaatcccttt ccaactcatc taggcatgga
    26401 aaggcaactg aaatcagaat ttttattaca ttcaatactt caaatcttat aattactatt
    26461 agtgataatg gtataggatg tggcaatata aaaaaaggaa atggacttaa tagtatcaat
    26521 gagagaatat atgaattaaa tggtaaagtt gaatttgata attctaataa tggttttaca
    26581 attagagctt cttttcctaa attttcaggg ggtgattttt ttgaataaaa ttaaagttct
    26641 tatagtagat gacgaaaaat taattagaaa aggtcttaaa attatattat catcttataa
    26701 tgatttagaa attgtaggag atgcttctaa tggatatgaa gctttagaat tttgtaaaat
    26761 gaatgatgta gacatagttc ttatggatat acgaatgaaa gtatgtgatg gggttctagg
    26821 tacaaggctt ataaaggaat ataataattc tataacatta ttaattttaa caacttttaa
    26881 tgatgatgaa tatataaaag atgctatgaa gtttggtgct tcaggatacc ttttaaaaga
    26941 tagctctgat gaagtattgc atgaagggat tcgttcttca ttttttggca atattgtgct
    27001 tgataaaagt gtagctgaaa aaatcatgac ctctgaaaaa acggttaaac aagagcatct
    27061 ttatgacatg tacaatttaa cagaaaaaga aatatctata attagactaa ttgcaaatgg
    27121 acttaataat aaagaaataa gtcaagaact attcttatct gaaggtacaa taaagaataa
    27181 cataactaat attttagcta aactagaact tcgtgacaga actcaactag caatctttgc
    27241 tttcaaaaat aaaattgtaa tagaataaat ctaaaaatat aaagtgactt aagtcacttt
    27301 ttttagtgac ttatagtaat agaattataa ttcacaattc tttattataa tattatagct
    27361 ttttaactta tacaaaatta ttttggaggg attactatgg atatagtcag agtaaataat
    27421 ataacaaaaa gatttaatga taaattagta cttgataaca tcagcttttc tgttaaaaaa
    27481 ggtgagatat ttggacttat tggcccaaat ggtgcaggta aatctacact tataaatata
    27541 attacaaatc ttatgttacc aaatagtggt agtattcaaa tcaatggttt agatttatca
    27601 aaagattata taaaagcaaa gtctataata gggttagttc cacaagaatt agcaattata
    27661 gaaactctta cttcttttga taacttagag tactttggag ctttctacgg tttaaaggga
    27721 aaactactta aagaaagaat cattgaagca ttagaagtta caggtcttac tgaagtcaaa
    27781 aagaaaaagg tcaaaaaatt atctggtggt atgcaaagga gattaaatat tggaatagca
    27841 ctcttaaatc atcctaaaat attaatattg gatgaaccaa ctgtaggtgt agacccccaa
    27901 tcaagaaatc atattttcaa ttttataaaa gatatatcta aaaaacatga aactactgta
    27961 atatatactt cccattatat ggaagaagtt gagcaccttt gttctaaaat ttttattatg
    28021 gatgaaggta aagaaattgc ttttggaaat aatgattatt taaaatcact cgtatctaca
    28081 aatacaaaat taattatgga aataaaaaat ataaatgccc aacttatctt tgatttaaaa
    28141 aatactaaag gtgttatatc tgttttagaa aataattcat tattagaatt ggatatagat
    28201 aaaaaattac aacttacaga tatactctct gtcattgaca aaaacaattc aaaaataatg
    28261 aaaatttctt atgaagaacc tagcctagaa gacgtatttt taaatctaac aggaaaaaat
    28321 ttaagggact aatggaggtg attttatgaa attatacatg agtgtaaaaa ctatgctaaa
    28381 gagtttaaaa agctccttta tacttaattt aatatatttt ttagctttgc ctctgatttt
    28441 atcttggttt cttgggatgg ttacagaaag catgtttcaa aaccctataa aaacagaatc
    28501 tacccccatt gttatatatg acaaagataa cacaagatta tcaaatgatt tgacaaagta
    28561 tttgaaaaat gatttatctt atatattaac agttaaaaaa gatgattcta aggcagaatt
    28621 aaagcttact atacctaaag gctatgagag tagtttatta aatgaaaaat caaatactct
    28681 aaacatagaa aaacttggaa ctcgtgatga tatcgcaata ttgcttcaag atatattaga
    28741 tacttaccat gaaaaatttt atttaaataa ttctcagaaa ataagttcag aagatttttc
    28801 taaattattt aataaaaatt ctattgatac ttctataata aataacaatg ttaaacagag
    28861 ctcttatgaa tactttgctt tagtttcact aggatttctt gtaataattt ttataatgaa
    28921 caatattttg tcaaattata taagtgaatc aaagggactt tcaaaaagac tttattctat
    28981 gcctataact agagtacaat tcttaattta tgactttgta ggtttatgga tttattcatt
    29041 tatattctta ttattatatg ttttattctt tagaatactt ggaataacat ttaaaggtaa
    29101 ttttgccata ttgctcctac tttgtgcttt atcatcttat tttatgactt caatatctac
    29161 ctttgtaacc agctttttta gcaaaaaata cggtacagca atagtttatg cattactctt
    29221 tttacagaca atttttggag gtatcttcag tatgataagt gatacattta ctaagcttac
    29281 aagtttatct cctacctatt taataggaga attgtttagt aattatgaaa catttaaaac
    29341 tatagattca ataggagatt taattttcac atgtttaatt atctctacaa tattaattgc
    29401 acttgctatt gtaaaggaaa attataaatg gagggaggtt taataatgat tagattaact
    29461 attgcaaata taaaaagata tctaaaaaat catactcttt taattaatat ggttatgctt
    29521 cctattatac taattttttc acttaatttt tttatcaaca actcgggaaa tcaatcaatg
    29581 tatttttctc cagtagcaat tgtatctgac tcttcaggaa agtatgaaca taagttaata
    29641 aatagttcta aattaaaaga aaattcattt agattaaatg aacaagataa ggctatgaat
    29701 ctattaaaaa ataataaggt ttctgctgta ttcgttttag ataaaaattt ttcaaatagt
    29761 atagataaat tacaacgacc tgtagttaaa tgctttaaaa ttgaaaatgg tggtggtagt
    29821 ctttgggcag aatcacaaat agaatctttt attactaaat cgttaaaatt gaaagtggat
    29881 aaaaatatag atgataaatt gacaaagaca aatataattg ataacaaacc agataagaat
    29941 aaaggttcat ttttagtagt cttcttgata tgctatttta tgtatattaa tgcggcacat
    30001 cttgcttctg atttatttac attaaaaaaa tcaaatgtgc taaaaagact tatctctaca
    30061 gataataaag atataaaaat aattttcagt atatttttag gactattttt tattcaatca
    30121 attgtataca cttttgctct attatgtttt tcaattatag aagactttaa tttgagtctg
    30181 aatgtattga tgattgtact agcaaatagt tttgtttcca ctggatttgt attttgggta
    30241 gctagagtat ttaaaaatga aagttctata agtcttatat ctacatttta ttctttaata
    30301 ggtctagcta tatctatctc tagtctaata ccatcaatgg ataaattatc ctttatgact
    30361 aatctttcaa agttcacacc attttattgg actatagatg ctattaaaaa caatggtagt
    30421 attttaataa atataatagc tctaatatta attggaatta tatttgtaac tgcaggaagt
    30481 cttaaactaa gagattttgc taaaaactaa taattattaa atataattag tatctatttt
    30541 tatataattg taaaagaata tttcaacata ttaaattatt ttatcttata tttttctctt
    30601 tagtttcaca catcaccaag tctaaacttt atattatata ttaatatttt tgacttgtca
    30661 agttatttat gttataatat taatcattag ataattaaag cattgtacct aagttgcaat
    30721 atctaaaatt caatttggag gtataactag catatgtata aaattggtga aagtgtaatg
    30781 tatccaaaag agggagcctg ctctgtaaat gatattgtaa caaagaaaat aaatcatgag
    30841 atgcaaaaat actatgaatt aagtgtcatt tttaatagta accttaaaat ttcgattcca
    30901 gttctaaatg cagatagaat tggcatacgt ccagttatgg atggaaatga tgttgataat
    30961 tttatccaat ctattaataa aacagatggt gtatggattt ttgatagaaa agaaagactt
    31021 aaactgtacc aagataaatt tcattcagga gatgtttttg aaattgtaaa attaatcaaa
    31081 atgcttatga tacaagacag ttcaaaacaa ttgtgtagca cagataagga gtttttaaat
    31141 aaagctcaaa aatttgcttt atcagaatta gctgctgctc aatgtaaatc ctatacaatg
    31201 gtattagagg aaatgaaaaa acatatatta aattcaaaga atataaatta aaccagtttt
    31261 ttaaactggt tttatttttt atactaatca tccatgctac tttagcttat tttaaactgc
    31321 ctaatctaat attaataaag ttatctcaat tagatagatg tattttattc aaataaaaaa
    31381 aaactaagag taaatcatat tactgattta ctcttagcta ataaaattta atcttcttca
    31441 agttcttttt tctcttgtaa taatacactt aattcatttg cttctaaaac ttgtgcctct
    31501 gcaatatcaa gtaactcttg ttgccattcc acacatttat ctttgtaatc aaaaggtagc
    31561 gttctgtcaa ctatgtactt atcaatacag tcctccgcac ttttaattat ttcttttctt
    31621 tcatcaataa atttttctaa ttctactaag gcttcttcat aagcttttct actcatataa
    31681 attccccctt tgtacaatta ttctacatta aaatgatttt tcctcttttt tgtataaaat
    31741 tttatttgta tataactttt ccatcaatat cttttatagt ttgacatcca gttaatatca
    31801 tagaactact tagctctgat tgaagtttat ttacataagt ttctacacca tcagttgcac
    31861 caccaaaaga agcagtcaca aaaggtcttc ctattaatac tgcatctgca cctaaaccta
    31921 tcatttttag tacgtctaaa ccagttctca ctccaccatc tgcaagtatt gtaactttac
    31981 ctttaacagc atcagcaatt tttggtaaaa cttcacatgc tcctggtgta cagtcaagca
    32041 ctcttcctcc atgatttgat acgactattg catccacacc tgcttctact gccattaaag
    32101 catcttcaac agtcattata cctttcaata taaatggtag tttagtagac tttactaact
    32161 cctttatttg tttaacattt ttaggaagta ctggttttcc atgtaatgat aaagttacta
    32221 gaccacaggc atctatatca actccaactg caaatgcacc agcttcttca gctaatctta
    32281 tcttttttat aatattttca ttatcccaag gtttaataaa tacaattcca gctccattat
    32341 actttttgac tacatctaaa ttttccatta aaaaagcatc tactgccgta tcaccaacca
    32401 ttgcataaat accactatta gcacatcctg caactactgg ttctatgtaa tctctttcat
    32461 taatttttcc acccatattt aaagtagttc ctgttactgg agctgcaaat ataggtgcac
    32521 tcattttctt tccaaacatc tctatagatg tatcaggatt tgatacatca tgtattactc
    32581 tcatatttac ttttactttt tctaaactct tgctattttc tataaaagaa gaaccacttc
    32641 ctttacctcc catgccaggc acttcacctg cacaagcaac cccattacaa accttacaaa
    32701 ctttacaact tccatttaaa ttttctctag cactttttaa taattcatta tattccatct
    32761 ttaatccccc attaactata gtaatttata ttatatacat tatatcatat tatatttaca
    32821 gtatattttt caaaaaactt tttctgtgga taggagttat gccatatttt tcaatagctt
    32881 catagtgttc tttagttcca tatcctttgt gagatttaaa cccatattct gggtatactc
    32941 tatcatattg gtacattata ctatccctag taactttagc aagtatactt gctgctgcta
    33001 ttgatataga ttttgaatct cctttcacaa ttggattttg agatatgtca atacctggta
    33061 ttgttgctgc atcaactaat aaataatccg gtgctttttt taaacaattt atagcctttt
    33121 tcattgccat atatgtagca tttaatatat taaattcatc tatttcttca ttgtttacta
    33181 tacctattcc ataatctagt gcttcttctt ttattacttc aaaaagttca tctctttttg
    33241 cctcactcag tttcttagag tcatttacac cttctatttt ggtatctttt ttaaatacta
    33301 caacagaagc tacaacaggt ccagcaagtg gtcctcttcc cgcctcatca attcctccaa
    33361 tatataaata acctttatca tagccttcgt tctcaaatat atttatagtt tccaatcttt
    33421 cttcttcttt tctaatatta tccagctttt ttgctagttt tatagctaga ccttgtacag
    33481 attttctttc atcaactctt aacagctcta tatattccat atatttttct acttctaaag
    33541 tctctattat ttctttaatc tccctcacac ttttatcctg cataccattc cctcatcttt
    33601 cgtttttaaa aatttaattt ttaaatatat taaaattttt taattagagt ttaattgcat
    33661 ccactatagc cacattttaa acatatacta catccacctg tatgtgcaat tccttcactt
    33721 ccacactcag ggcacacaaa tttttcatct tcttcaaaat ttttatttat tatttctttg
    33781 gctttttcat ctttattatc tttttctttt ttattatttt tataatattc ttttttacta
    33841 ttttcttttt tatcttcttt attattttct tccttttttt catttattaa aattccactc
    33901 cttttacatc catctctaaa tattgttact cctttaagac cagcctccca tgccttaata
    33961 tatatattga aaacatcttc aactgtagtt tcaaaaggta tatttattgt agatgatata
    34021 gaagcatcaa tatacttttg ccatactttt tgcatgttta ttctttcttc atatcctata
    34081 ttcatagcag taacaaaaaa atctggtaaa cattcttcat ctgtaatacc tttcttgtcc
    34141 atatactctt taacaattgg agtatatact ttataataca catcctcatc atgcaagctt
    34201 tcagtttttc ttatatatga aagattaaat ataggttcta ttccaccact gatacctatc
    34261 attgtagata tggaacctgt aggaggtatg gttaaaagtt gtgaatttct aagcccatac
    34321 tttttaacca tttccttagt gtcttcattt agattttcta taaaaaactt agatttagaa
    34381 atattttcat atttatactc actaaatgtt ccatattctt tagcaattaa tgctgattgc
    34441 ttcacagctt catttaacat agtcttacca attaaatcac atagttctaa agattcatca
    34501 gaaccatatc ttattcccat ttttataagc atatcagcta tacccattac acctaaacct
    34561 atctgtctat acttatgtac actatctctt tgttcttgta atggatgcaa gttcaatccc
    34621 tcatctaaga catcatttaa agctactaca gcttctctaa cgcaagattt taatttatta
    34681 atatcaaaag tagcatgttc accaaatggt tgtattataa actctgacaa gtttattgag
    34741 ccaagtagac aacttcctcc acttggaagt ggttcttctg cacaaggatt tactcctgaa
    34801 aagcaaaagt tcttatcttc actcataaaa ttatatgttt ttattttatc ccaaaacaat
    34861 atacctggtt ctgcataatc ccagttattt ttcacaaatt tattaaataa atcataagca
    34921 tttactatct taactatttt ttctccagta gtttcaactt caaaagtaca attatataac
    34981 tttttttctc ttacagcttg cataaattca tctgttactc ttacagaaat atttgcttta
    35041 gtaaccttct ccaaatctgt ttttatatct ataaagtcct ctatctctgg atgatttaca
    35101 tccatagaaa tcataagagc tcctcttctc cctctttgac caattaagcc agttgtcata
    35161 ctgtaaagtt ccataaaaga gactgctcca gtcgtatgtt ttgcagcatt gttaactttc
    35221 gctccttttg gtcttagctt tgaaatatca attccaactc ctccaccata actaaaagtt
    35281 ctagcaagtt tttttgcagt atcaaatata tcttctatgc tatcttttgg tggttctatt
    35341 acataacaat tagaatatgt tacttttttt ccttctttct gtaatcctct atttgctaaa
    35401 attcttccag caaatagaaa tcttttttgt cttattagtt ttgatattct tgaactatta
    35461 tttgaaattc tctttatcca ttcatcaaaa ctctcaccat tatgtctata cttgttcttc
    35521 catattgatt tttgtaattc atcaaactcc caagctttag tccttatttc agagcgtctt
    35581 tctctatata atatgtagtg ttttgctata tcttttctat ttgattccat aagtagtact
    35641 tcaactatat cttgtatttc ttctacggat aagcattttt ccatagaatt atagtcatta
    35701 gctatttttc ttgatatatc atatgccaac tctctatcta ctccaccttc actatttata
    35761 ctagctttat agacagcatc ttcaatctta cttttactaa aaagttcttt tgtaccatct
    35821 ctttttatta tgtacttcat ttttaaacct ccaataaatt agtattaaat taatacacac
    35881 attaactata actctaaaca aacttttt
//
LOCUS       DAEOSP010000018        35761 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.018, whole genome shotgun sequence.
ACCESSION   DAEOSP010000018 DAEOSP010000000
VERSION     DAEOSP010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35761
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.018"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(230..742)
                     /locus_tag="KRH54_000697"
     CDS             complement(230..742)
                     /locus_tag="KRH54_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG8578369.1"
                     /translation="MLEVIKNRHSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPVKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            1192..1668
                     /gene="purE"
                     /locus_tag="KRH54_000698"
     CDS             1192..1668
                     /gene="purE"
                     /locus_tag="KRH54_000698"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBG8578370.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            1674..2372
                     /locus_tag="KRH54_000699"
     CDS             1674..2372
                     /locus_tag="KRH54_000699"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG8578371.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            2384..3751
                     /gene="purF"
                     /locus_tag="KRH54_000700"
     CDS             2384..3751
                     /gene="purF"
                     /locus_tag="KRH54_000700"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBG8578372.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            3745..4821
                     /locus_tag="KRH54_000701"
     CDS             3745..4821
                     /locus_tag="KRH54_000701"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBG8578373.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            4815..5408
                     /locus_tag="KRH54_000702"
     CDS             4815..5408
                     /locus_tag="KRH54_000702"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBG8578374.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            5401..6933
                     /gene="purH"
                     /locus_tag="KRH54_000703"
     CDS             5401..6933
                     /gene="purH"
                     /locus_tag="KRH54_000703"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBG8578375.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            6944..8194
                     /gene="purD"
                     /locus_tag="KRH54_000704"
     CDS             6944..8194
                     /gene="purD"
                     /locus_tag="KRH54_000704"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBG8578376.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            8219..12025
                     /locus_tag="KRH54_000705"
     CDS             8219..12025
                     /locus_tag="KRH54_000705"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBG8578377.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLRTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDDKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVLGSTVKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSVDGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            12231..13103
                     /gene="rfbD"
                     /locus_tag="KRH54_000706"
     CDS             12231..13103
                     /gene="rfbD"
                     /locus_tag="KRH54_000706"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBG8578378.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENTEVNVVNDQFGTPTSTVDLAKIIIKIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            13212..14081
                     /gene="rfbA"
                     /locus_tag="KRH54_000707"
     CDS             13212..14081
                     /gene="rfbA"
                     /locus_tag="KRH54_000707"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBG8578379.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            14130..14687
                     /gene="rfbC"
                     /locus_tag="KRH54_000708"
     CDS             14130..14687
                     /gene="rfbC"
                     /locus_tag="KRH54_000708"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBG8578380.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            14725..15708
                     /gene="rfbB"
                     /locus_tag="KRH54_000709"
     CDS             14725..15708
                     /gene="rfbB"
                     /locus_tag="KRH54_000709"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBG8578381.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            15738..16406
                     /locus_tag="KRH54_000710"
     CDS             15738..16406
                     /locus_tag="KRH54_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBG8578382.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVDQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            16415..16843
                     /locus_tag="KRH54_000711"
     CDS             16415..16843
                     /locus_tag="KRH54_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBG8578383.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            16867..17181
                     /locus_tag="KRH54_000712"
     CDS             16867..17181
                     /locus_tag="KRH54_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG8578384.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNERLSVRLSKIEDPD
                     VN"
     gene            17333..17608
                     /gene="flgM"
                     /locus_tag="KRH54_000713"
     CDS             17333..17608
                     /gene="flgM"
                     /locus_tag="KRH54_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBG8578385.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            17613..18029
                     /locus_tag="KRH54_000714"
     CDS             17613..18029
                     /locus_tag="KRH54_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBG8578386.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDGYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            18047..19357
                     /gene="flgK"
                     /locus_tag="KRH54_000715"
     CDS             18047..19357
                     /gene="flgK"
                     /locus_tag="KRH54_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBG8578387.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            19376..20308
                     /gene="flgL"
                     /locus_tag="KRH54_000716"
     CDS             19376..20308
                     /gene="flgL"
                     /locus_tag="KRH54_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBG8578388.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            20324..20716
                     /locus_tag="KRH54_000717"
     CDS             20324..20716
                     /locus_tag="KRH54_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBG8578389.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDSLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            20710..20922
                     /gene="csrA"
                     /locus_tag="KRH54_000718"
     CDS             20710..20922
                     /gene="csrA"
                     /locus_tag="KRH54_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBG8578390.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            20944..21339
                     /gene="fliS"
                     /locus_tag="KRH54_000719"
     CDS             20944..21339
                     /gene="fliS"
                     /locus_tag="KRH54_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG8578391.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DSVLPVIEQIKDTWHEADKKSKEERSRDK"
     gene            21339..21695
                     /gene="fliS"
                     /locus_tag="KRH54_000720"
     CDS             21339..21695
                     /gene="fliS"
                     /locus_tag="KRH54_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBG8578392.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQNAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            21716..23239
                     /gene="fliD"
                     /locus_tag="KRH54_000721"
     CDS             21716..23239
                     /gene="fliD"
                     /locus_tag="KRH54_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBG8578393.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSNEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKATYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVASVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     ITDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            23242..23577
                     /gene="fliT"
                     /locus_tag="KRH54_000722"
     CDS             23242..23577
                     /gene="fliT"
                     /locus_tag="KRH54_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBG8578394.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            23679..24551
                     /locus_tag="KRH54_000723"
     CDS             23679..24551
                     /locus_tag="KRH54_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HBG8578395.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            24644..26785
                     /locus_tag="KRH54_000724"
     CDS             24644..26785
                     /locus_tag="KRH54_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG8578396.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYFYIMYSYKYIRYIYSNLSDFELKKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKYSFIISIENMIETGKINDAKLLVDDYSKTFVDDVRILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            26809..28686
                     /locus_tag="KRH54_000725"
     CDS             26809..28686
                     /locus_tag="KRH54_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG8578397.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            28679..30793
                     /locus_tag="KRH54_000726"
     CDS             28679..30793
                     /locus_tag="KRH54_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG8578398.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     TSQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKAKQSEIKHFVIDKHCIEIKNYIDKIYNEVNHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            30802..31797
                     /locus_tag="KRH54_000727"
     CDS             30802..31797
                     /locus_tag="KRH54_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HBG8578399.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLQEQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            31889..33121
                     /locus_tag="KRH54_000728"
     CDS             31889..33121
                     /locus_tag="KRH54_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBG8578400.1"
                     /translation="MKRLMILGSIKYFENIVMSAKREGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKNIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAVMKNYFKNNGIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLIYPSKYCFQYEEEIKSIYQNITQAFGIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGVANNVFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            33141..34085
                     /locus_tag="KRH54_000729"
     CDS             33141..34085
                     /locus_tag="KRH54_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HBG8578401.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHKIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            34179..34586
                     /locus_tag="KRH54_000730"
     CDS             34179..34586
                     /locus_tag="KRH54_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HBG8578402.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNV"
     gene            34673..>35761
                     /locus_tag="KRH54_000731"
     CDS             34673..>35761
                     /locus_tag="KRH54_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBG8578403.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHFEEE
                     FANYVGSKYCVGLASGLDALIIAFRALGISKGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKSGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFIGMEDEEVSYIIKIINEY"
BASE COUNT        14645 a         3592 c         6535 g        10989 t
ORIGIN      
        1 aaaatacttt aatgaaaaat agctaacttt aattgtagga tattcttaat atatttgaaa
       61 taaataaaaa tattgaagga tataatattc aattggtata taaataataa atgatagtta
      121 aattttacct tagaggctaa ttaggtgttt aatcaaagtt aatgccttta gaatacaaat
      181 gtattctaaa ggcattttta ctatgtattt tcatttatat tactaaacat tatctatttt
      241 tatatacatt tctatgtatt ttattttcat caaatcggtc tataaactca tttttttctt
      301 cactatatcc aatagatata agtgcataag gaactaattt tactggtaag tctaaagttt
      361 gtctaacttt atttaccact ttatttttag gaaatactcc tgcccaacaa gcagctagac
      421 cttgattagt aacttctaat aataaatttt gtgtgcaagc acctaaatct tgtatccatt
      481 taccaggttt taagaattta tctctatttc caagcactac tatacataat ggtgcaaatt
      541 ctatatgctt tgctctatgc tgtgactttg agagctgttt cagcagttcc ttatcatcta
      601 ctataataaa ttcccaaggt tgagcatttt ttgatgaagg tgcttgcata gctgatttta
      661 gtatttcagt tattttatct tcttctatat ttttatctat atatgttctt attgaatgtc
      721 tattttttat aacttctaac atacagattc tcctttaaat aaaattattt ctatttatat
      781 aatatcatta ctttaaattt tagctattac ttatataaag aataaacttt gtgttggttt
      841 tatctaagta aaagtaacat tcagaactaa aaaacaagac tataaatcca aagtattaat
      901 taaaaaagaa gtaataaaaa ataatataca agatttaaaa aagagtattg ggcttaaaat
      961 ttaattagaa aaccaatttt gagataatct gttttttatt ttaacaaaaa aacataaata
     1021 aagttcgata catagcgaat gaaaaaatta caagaaatat tttttttaaa aaatttcaaa
     1081 aaagagttga taatatatgt atattaatat aaaatttaag gtgtataatt cgtataataa
     1141 tacgaatgat ttgtagtgta aaataactta taaacggaga ggggaagtct aatgaaagta
     1201 gcagtagtta tgggttcaaa atcagattat ccaaaattag aagaagggat aaaactgctt
     1261 gaaaaatatg gaatagaagt tgtagcaaga gcattatcag ctcatagaac accagaacaa
     1321 ctttcaatat tcctaaaaga aatagaagat gatactgatg taataatagc agcagcagga
     1381 aaagcagcac acttaccagg tgtaatagct tcacaaacat taattcctgt aataggatta
     1441 cctattaaat catcaactat ggatggacta gactcacttt tatcaatagt tcaaatgcca
     1501 aaaggaatcc cagttgcaac agttacaata gacttaggtt taaacgcagc tttattggca
     1561 ttacaaataa tgactttaaa atacccaaaa ttaaaagagg atttgaaatc atatagagaa
     1621 gagatggcac aaaaggtgtt agaagacgac aagaatttaa gggggtagca gaaatgttat
     1681 tatatgaagg aaaagcaaaa caagtatatt ctacagataa tgaaaatgaa tatgttgttt
     1741 attataaaga tgatgcaaca gcatttaatg gagaaaaaaa agcagaaata tcttcaaaag
     1801 gtatactaaa taataagata tcaacaataa tctttgaaat gttaaaagaa aataatataa
     1861 atactcactt tataaaaagt ttatcagata gagaaatgtt agttaaaaaa gtagagattt
     1921 taccattaga agtaatagta agaaatattg ctgctggttc tatctgtaaa agagttggtc
     1981 ttgaagaagg tgtagttttc gatgagccaa tttttgaaat aagctacaaa aatgatgctt
     2041 atggtgaccc aatgctaaat gatgattatg cagtggcaat gaagttagca actagagaag
     2101 agttaaaatt cttaagagaa gaaactttaa aaataaacga gttactaaaa gcatttttct
     2161 taaaattaaa cctaaagtta gttgacttta aaatagaatt tggtaaagat tctgaaggaa
     2221 atataatatt ggcagatgaa gtatcaccag acacttgtag actttgggat gtaaatacaa
     2281 atgaaaagtt agataaggac agatttagaa aagaccttgg agaccttgta gaaggttata
     2341 cggaagtact ttcaagaatg aataataaat aggaggcaaa tgtatgtgtg gagttttagg
     2401 gatttactct aataaagatg taactaaaga actatattat tctttatatt ctatgcaaca
     2461 cagggggcaa gaaagctgtg gtttagctct tttagatgat ggagaaatta agtacaaaaa
     2521 agatatggga ttagttggag atgtttttaa ggaaaatgaa ttatctaagt taaaaggaaa
     2581 tatagggatt ggacatgtta gatattcaac agctggagga agtcatgttt ctaactgtca
     2641 acctttagtt ggaagttgta gaaaaagaca attagccata gctcataatg gaaatctagt
     2701 taatgcaaat tatttaaaag atatgctaga agaagatgga tatatgttcc aaactaattc
     2761 agatacagaa gtgattttat atatacttgc tagatattac aaaggggata tagtagaaag
     2821 tttaaaagtg acaatggatt atataaaagg tgcatatgct cttgtaataa tgagtcaaga
     2881 agaattggtt gcagttagag acccacatgg attcagacct cttgtacttg gtaaaaaggg
     2941 tgatgaatat atatttgcat cagaaaattg tgcaatagat atacttggcg gagaagttat
     3001 aagagatgta gagccaggag agataatagt agtcaaagat ggagaactaa aatcatattt
     3061 ttattcagaa aattataaac cagttaagaa aagttgtata tttgaacaca tatactttgc
     3121 aagaaatgat gcaactatag acaatgtaaa tgcatatgaa tttagaatta aatgtggtga
     3181 gagattagct cagaatgaaa ctgtaaaggc agatatggta gttccagttc cagattcagg
     3241 ttggccagga gctattggtt atgcaaatgc tagtggatta aaaataagtg aaggcttagt
     3301 taaaaataga tatgtaggaa gaacatttat aaaacctaca caagaagaaa gagaaatagc
     3361 agtaaaaata aaattaaatc ctctttcaac aataataaaa ggaaaatcta taattttagt
     3421 agacgactca attgtaagag gaactacatc taaacaatta gtaaaatctt taagagaagc
     3481 aggagcaaaa gaaattcatc ttagaataac ttctcctcca gtagcttact cttgttacta
     3541 tggaatagat actccaaatc gctctaaatt aattgcatca agtaacaacg tagaagaaat
     3601 gagagaatat attggatgtg atagtttaaa attcctagat atagaaggaa tgttagatgc
     3661 aacagaacat aagtctactt tctgtaaagc ttgttttgat ggagaatacc cagttaagaa
     3721 aattgataag gaggaactac tttcatgtta acatataaag aatcaggtgt agatatagat
     3781 gaaggaaata gagcagtaga ccttataaaa ggtaaaataa aaggaactta tgatggaaat
     3841 gtagttggag atttaggtaa cttcagtgga ttatacagtt taaaagattt tgtaggtatg
     3901 aaagaaccag ttttgcttgc atctacagat ggtgttggga ctaagttaaa aatagctcag
     3961 atgatggaca agcatgatac tgttggaata gatttagttg caatgtgcgt gaatgattta
     4021 atttgtcaag gagcaaaacc attattcttc cttgactata tagctcttgg gaaattagtg
     4081 cctgagcata tcgaaaaaat agttggtgga attgcagatg gttgtaaaat gtctggttgt
     4141 gcactaattg gtggagaaac tgctgaaatg cctggaatgt atggagaaga tgactatgat
     4201 ttagcaggat tttctgtagg aatagcagac aaagaaaaga ttgtttctgg aaacaatgtt
     4261 aaatcaggag atgtattggt tggtatatct tcaagtggag tacacagcaa tggattttct
     4321 tttataagaa agatattttt agaaacttac aattataaaa tggaacaata tgttgaagaa
     4381 ctaggaatga cagttggaga agcactttta acaccaacta aaatatatgt taaattagct
     4441 ttagatgtat tagctaaaca tgacataaaa gctatagctc acataactgg tggagggctt
     4501 atagaaaata taacaagagt tatacctaaa gggttaggac tagatatcaa taaaaaatct
     4561 tgggagaagc ctcctatatt taaaatgata gaaggtttta atgctgttga tgagagagaa
     4621 cttcataaga gttttaacat gggtattggg ctagttttaa tagtagataa agaaaatgct
     4681 gatgatgttg taaactttat aaataataga gaaaatgaca atgctgctta tgtagataaa
     4741 aaatatagtg aattattaga agataaagct tatataattg gtgaagtagt agatagtcat
     4801 gaaggtgtag aattatgcta aatataggag ttttaatatc tggtggagga acaaatttac
     4861 aagcagttat agatggtact gagtctggag aaataaaagg tcaggttaaa gtagttatct
     4921 caagtaagca aggtgcttat gggctagaga gagctaaaaa tcataacata aaagcaattt
     4981 gtgaaactga tgaagacaag ataatagaaa ttcttaagga aaataaaata gatttagtag
     5041 ttttggctgg ttatttaaaa ataatcagcc caaagctggt taacgagttt agaaacaaaa
     5101 tgataaacat acatccatca ttaattcctt cattttgtgg agctggattc tatggagaaa
     5161 aagttcatca aggtgttata gattatggag ctaaagtaac tggtgctaca gttcattttg
     5221 tagatgaagg tgctgatact ggaccaatca taatgcaaga tgtagttaag gtaaatcaag
     5281 atgatgatgc aaagacactt gcaaaaagag tgttagaagt tgaacatagg attttaaaag
     5341 aaagtataag tcttttttgt gaaaataaat taaaacttca aggtaggaga gtgtttataa
     5401 atgagtaaga gagctttaat aagtgtaact gacaagactg gtgtagtgga atttgctaaa
     5461 gagttaaata aattagatta tgaaattata tctacaggaa atacatttaa aacattaaaa
     5521 gaaaatggtg ttaatgtaat gcaagttgaa gatgttacaa attttccaga aatattagat
     5581 ggaagagtaa aaacattaaa tccatatata catggtggta tactttataa aagagataaa
     5641 gaatctcatg tagaaactgt aaatgaacac aaaatacatt caatagattt agtagctgta
     5701 aatctatatg attttgaagg aactttaaaa gctggaaaaa gtcatgatga aataattgaa
     5761 aatatagata ttggtggacc atctatgata cgttctgctg ccaaaaacta taaggatgta
     5821 atagtagtag tagatataaa agattatgat tctataatag aaaaattaaa aacagataca
     5881 atgacattag aagacagaaa aaaattatca tataaagctt tctcaactac aggaagatat
     5941 gatgcgttaa tatctagtta ttttgctgga gaagttggag atacgtatcc tgacatatta
     6001 aatttaactt tccaaaaaga acaaacttta agatatggag aaaatccaca tcaaaatggt
     6061 ttcttatatt cacagtcaaa tgcaaaaaat ccaatcttaa attatgagca attaggcggt
     6121 aaagaacttt cttttaacaa cttaaatgat ttacatggat gtttagaagt aatgagagaa
     6181 tttaaagata gtgaagaagt agtttcagta gctataaaac atgctaattc atgtggggta
     6241 ggtttaggaa aagatgcttt tgaagcatat acaaaatgtt atgaagcgga taaggtttca
     6301 atatttggag gaatagtagg aataacttct actatagata aagctacagc agagaaatta
     6361 aatgaaatat tcttagaaat agtagtagca tatgattttg aaccagaagc tttagagata
     6421 cttaaacaaa agaaaaatct aagaatatta aaattagcta aaatagaaaa tagtttacaa
     6481 ccatatgaaa tgaaatattt agatggaaaa ttattgatac aagatagaaa caatatatta
     6541 gcagaaaaaa gtgaaaatgt aacaaaggaa aaaccaacag atgcacaact taaggacatg
     6601 gaatttggta tgagagtagt aaaaaatatg aaatcaaatg caattgcaat agtaaaaaat
     6661 ggacaaactc tagctttagg ttgtggacaa acatcaagaa tttgggcatt aaaaaatgct
     6721 cttgaaaata acaaagataa agattttaca ggagctgttt tagcgtcaga cgcatttttc
     6781 ccatttgatg attgtgtaac attagctcat gaatatggaa tatcagcagt agttcaacca
     6841 ggtggctcta taaaagataa agattcaata gaagcttgtg acaagtatga tatggttatg
     6901 gtatttacag gaataagaca cttcaaacac taaggaggat attatgaaga tattggttgt
     6961 aggcggtggc ggaagagagc atgctatatg ctggaaacta agcaaagaaa aaaatgtaga
     7021 aaaaatttat tgtgcaccag gtaatgcagg aattgcaaac gtagcagaat gtgtaaatat
     7081 aggtgatact aatatagaag aacttttaaa atttgccaaa gaaaatgaaa taggtctgac
     7141 aatagtagga ccagaagtac cactagttat gggaatagta gatgagtttg aaaaagaagg
     7201 attaagagta tttggaccta ataaaaaatg tgctcaactt gaaggaagta aagctttttc
     7261 aaaagaattt atgataaaac ataacatacc tactgcaaaa tataaagaat atacaaactt
     7321 agaagaagct atatcagaaa tagatagttt tggatatcca gtagtaataa aagcggacgg
     7381 attagctgct ggaaaaggtg ttgtaatacc tgaaaataga gaagatgcta tagcaacttt
     7441 aaaagaaatg atgtcagata agaagtttgg agctgctgga gataaaatag taattgaaga
     7501 gtttttaaaa ggaatagaaa cttcaattct agcatttgta gacaatgata caatagttcc
     7561 tatggcaagt gcaaaagacc ataaaaaagt aaacaactat gagcaaggtc caaacactgg
     7621 gggaatggga actttttcac ctagtgaaat atatacagaa gaattagcaa ataaagtaaa
     7681 agagacagta ttagaaaaaa ctttagaagg cttcaaaaaa gatggactta attttaaagg
     7741 tattttattt gttggtctta tgataactga agatggagaa aaagtacttg aatataatgt
     7801 aagatttgga gaccctgaga cacaatctgt tttatttaga ttagaaacag atttacatga
     7861 gataatggaa gctatactag ataataagtt aaaagatata gaaataaact atagtgatga
     7921 tgaagctgtg tgtgttatgc ttacatcagg agggtatcca gacagttatg aaaaagggaa
     7981 aattataact ggtcttgaaa acttggatga tgatatagta gtattccata gtggcactaa
     8041 gatgtttgat ggtaatttgg ttaccaatgg aggaagagta attggtataa ctgcaaagtc
     8101 aactacagtt aaggatgcag cagaaaaagt ttatgaaaac ataaagaaga taaactttga
     8161 aggaatgcac tatagaacag acataggaag ataatttttc taaaatggag gataaggtat
     8221 gttgaatact gaaaataaag actcaatggt tagaagagta ttggttgaaa aaagagaagg
     8281 ttttgacctt gaggctaaag ctttaaaaaa agacttagta gaaagtttac acatagataa
     8341 tatagaaaat ttaagaatat taaatagata tgatgtagaa ggaatatcag aagaagttta
     8401 tgaaaatgca gcaaagacaa tattctctga accaaattta gatgtggtat attatgaaga
     8461 aataccaaaa ttaaatgatg aaagagtatt tgcaatagaa tttcttcctg gtcaatacga
     8521 ccaaagaggt gattgggcag ctcaatgtgt acaaattgta aatcaaggga taagacctgc
     8581 tattaataca gcaaaagttt atatattatc aggcaaaata actgatgaag agttttctaa
     8641 aataaaagat tactgtataa atccagtaga tagtagagaa gcatctttag agaaaccaga
     8701 aacgttaaaa atggaaactg aaataccaac aacagttgaa gtattagatg gatttataga
     8761 tttagatgaa aatggattaa gaacatttgt aagtgaaaaa ggattagcaa tgactttagg
     8821 agacttacaa cacgttcaaa aatactttaa agatacagaa aagagaaatc caactataac
     8881 agaaataaaa gtattagata cttattggtc tgatcattgt agacatacta cttttatgac
     8941 tgaaatagaa aatgtaaaga tagaagatgg taagtttaat gatatagtaa aagaagcgta
     9001 tcaaatgtat cttaactcaa gagacaatgt atatgtgaac agacataaag atatatgttt
     9061 aatggatata gccactgttg cagtaaaaga attaaagaaa aatgggaaat taaatgacct
     9121 agatgaaagt gaagaaataa atgcatgtag tataaacgta gatgttgaag ttgatggtaa
     9181 gatggaaaaa tatctagtaa tgtttaaaaa tgaaactcac aaccatccaa ctgaaataga
     9241 accatttggt ggagcagcta cttgtttagg tggagctata agagacccat tatcaggaag
     9301 aagttatgtt tatcaagcaa tgcgtgttac aggaagtgct gacccaagaa cgactttaga
     9361 agatacttta cctggaaaac ttatgcaaag aaaaataact acagaagctg ctcatggata
     9421 tagttcatat ggtaaccaaa taggtcttac aactgggcaa gttgcagaag tatatgatga
     9481 aaactttgtt gcaaagagaa tggaaatagg tgcagttata gcagcagcac caaaagagaa
     9541 tgtagtaaga gaaagacctg aagcaggaga tgtaatcgtt cttcttggag gaaaaacagg
     9601 aagagatggc tgtggaggag caactggttc atctaaagaa catagtgaag agtcaatctt
     9661 aacttgtagt gcagaagttc aaaaaggaga tgctcctaat gaaagaaaaa tacaaagatt
     9721 ctttagaaat aaagaagttg ctcaaatgat aaaaagatgt aatgattttg gagcaggtgg
     9781 agtttgtgtt gctataggag agattgctga tagcttagat ataaacttag acttagttcc
     9841 taaaaaatat gatggacttg atggaactga gcttgcaatt tcagagtcac aagagcgtat
     9901 ggcagtagcc ataaaaaaag aaaataaaga taaatttata caattagcag tagaagaaaa
     9961 cttagaagct acgcatgtag ctacagtaac agatacagga tatttaagaa tgttctggaa
    10021 tggaaaagca attgtagata taaatagaga gtttttagat acaaacggag taaaacaaac
    10081 tactgatgtt catgttacta aagtagatga agaaaataca ttctttagct ctaatgaaat
    10141 agtaaaagat gttaagtgtg caagtatgaa agacaagttt acaaaagtac tatctgacct
    10201 taatgtttgt tctcaaaaag gattagtgga gatgtttgat aatacaattg gtggtaatac
    10261 agtacttatg ccatttggag gaaagtatca agcgactcca acacaaggta tggttgctaa
    10321 gataccagta cttggaggag agactaatac ttctacaata atgacttatg gatacaatcc
    10381 aaaggtagga aaatggagcc cattccatgg tgccttgtat gcagtagtag aatctgtgtg
    10441 taaattggtt gctattggag gaaattatag tacaacaaga cttacattcc aagagtattt
    10501 tgaaaaactt ggtaacaatc cagaaaaatg gggtaaacca ttctcagcac ttttaggagc
    10561 attttatgca caaagtaaat ttgagatacc tgctataggt ggaaaagaca gtatgtctgg
    10621 tacatttaaa gatattgaag ttccaccaac attggtttca tttgctgttg acacagtaga
    10681 tgcgaagaaa gtagtatctc cagaatttaa aaaagcagac tcaaaagtag taatgttatg
    10741 tgtaaacaag gcagaaaatg atgttgtaga ttttgaggaa ttaaagagaa acttagataa
    10801 agtaagagaa cttatacatg gaaataaagt tttatcaaca tatgcattag gatttgcagg
    10861 tgtaggtgaa gcaataagta aaatggcttt tggtaataaa ataggattta agtttagtga
    10921 agaggctgaa aaagcattta cagatgataa attatttgag gctagttatg gaaatatagt
    10981 acttgaatta gcaaatgatg atttaagtat gttagaagga tacaattatg ttgtgcttgg
    11041 aagtacagtt aaagaagcta gtatatttat caaaggtgaa gaacttgcat tagatgaatt
    11101 atataaagca cattgtagta ctttagagcc tatattccca actaaaacag aagaagtaaa
    11161 atcaaagata gaaactataa gttatatatc acaaggagaa gctaaaaaat catcattaag
    11221 tatagctact ccaagagtat ttataccagc attccctgga acaaactgtg aatatgactc
    11281 tgcaagagca tttgaaagag caggagctaa tgcaagtata agagtattta agaatttaac
    11341 ttataaagac atagaagatt ctatagatac aatagtaaac gaaataaaat cttctcaaat
    11401 aataatgtta ccaggtggat ttagtgctgg tgatgagcca gatggttctg gtaaatttat
    11461 agctactgta tttagaaatc ctagagtgca agaagctata aatgaatttt taactcaaaa
    11521 agatggacta atgttaggta tctgtaatgg attccaagta ttaattaaat tagggttagt
    11581 tccatatgga gaaataagag ttccaagtga aagtgctcca acacttactt acaataatat
    11641 tggtcgccat caagctaaga ttgctagaac tagaatttca tctaataaat ctccttggtt
    11701 agctcaaact aatgttggag atattcataa tatagcaatt tctcatggag aaggtaagtt
    11761 tgttgccagt gaagatgtta tgagagaatt aattgctaat ggacaagtag caactcaata
    11821 tgttgacttt aataatgaag ctacttatga tattgagttt aatccaaatg gttcattcta
    11881 tgctgttgaa ggtataacta gtgttgatgg tagagtgttt ggtaaaatgg gacattctga
    11941 aagaattggt gaggaagtat ataagaatat tataggtgaa aaagaccaaa agatatttga
    12001 atctggagtt aaatatttta gataaagctt tgatgtttaa ttgatagcta gaatatataa
    12061 tttagatatt tagatttaaa gagatgtaat aatatgatga taatcattta atattacatc
    12121 tcttttttat ttatctaaga aataataagt tcaacctaag ttaaaataca agctgtgtta
    12181 taattaatta ttaagtaaaa ttgaatattt attggagggc aacataatta atgaaaatat
    12241 taataacagg gtctaatggt caattaggaa aagaattggt taaccaatta gaagcaatca
    12301 atcaaagtat aaaccaacca aaatatgtta tattagctac aacaagaagt gatttggaca
    12361 tatccaatca gactaatgta gataatttta ttttgcataa taaacctgat gtagttgtaa
    12421 actgtgcagc atatacaaag gttgatgcat gtgaagataa tatcgaaatt gcatacaaaa
    12481 taaatgcttt aggagtgaga aatctagcaa tagcatcaga aaaagttaat gctaagctta
    12541 ttcacatatc aactgactat gtattcaatg gtttttctaa atatccatat agggaagaca
    12601 ataaaactga acccaattct gtatatggaa aaagtaaact tatgggagaa aaatttgttg
    12661 aacaattctc acataagtac tttatattga gaacagcatg gttatatggt aatgggaata
    12721 attttgtaaa gaccatgata aaactttctt tagaaaatac agaggttaat gtggttaatg
    12781 accagtttgg aactccaaca agtacagtag acttagcaaa aattataata aaaatcatgg
    12841 aaacagaaca ttatggagtt tatcatgcta catgtgaagg tgagtgtagt tggtatgatt
    12901 ttgccaaaaa gatatttaaa ctaaaaaata tagatataaa agtaaatcca attaagagta
    12961 acgaatttaa atgtaaggct ccaagaccac tatattctgt attggataat tttatgttga
    13021 agttaattgg tttaaattct tttagaaagt gggaagaatc cattgaagaa tatttagaaa
    13081 gatatgatat aaatgaaaag taaatgtatt attttgagaa aagaaaataa gatattataa
    13141 aaaatataaa tatttctttt atttatccga taatcataat atgacaaaaa caaatttaag
    13201 gatggaaaaa gatgaaagga attattttag caggagggtc tggtacaaga ctatatccta
    13261 taacaaaatg tatatcaaaa caaacacttc caatatacga taaaccaatg atatattacc
    13321 ctatgtcggt tcttatgttg gcagggatac aagatatatt aataatatct acaccaagag
    13381 atataaaagc atttgaagaa ttatttggag atggaagttc actagggctt aacataacat
    13441 atgcaattca ggagaaagca aatggaattg cagaagcgtt tataatagga gaagaattta
    13501 taggaaatga cagagtgtct cttgtattag gggataatat attttatgga tatggattta
    13561 gtgaaaggtt ggagaatgca gttaaaagaa aaggagcaac tatatttggg tatcatgtaa
    13621 gtaatccaaa ttcgtttgga gttgtagaat ttgataagga ttttaatgta ttgtcaatag
    13681 aagaaaaacc tacagttcct aaatctaatt atgctatacc aggattatat ttttatgata
    13741 atgatgttgt agagatagca aagctagttg aaccgtcaga tagaggtgaa ttagaaataa
    13801 cctctataaa cagtgaatat ctaaaacgag gaaaactaaa agtggaactt ctaggaagag
    13861 gtatggcttg gcttgatact ggtacacata aaggtctatt ggatgcttct aattatgttg
    13921 aagtggttca aacaagacag ggactgtata tagcatgttt agaagagatt gcatatacaa
    13981 aaggatatat aagtagagaa caattaatga aactagccaa gccactgata aaaactgaat
    14041 atggtcaata tctattaaaa ttggctagtg aagatatgta atatggtaga taaaatttat
    14101 tataaatttt attaagggag tttattataa tgggaaaaat caatgttata aagactaaaa
    14161 tagaaggatt atatatagtc gaaccaaatg tattttatga tgaaagagga tattttatgg
    14221 aaagctataa taaaaaggat tttatagagc agggtcttga tatgtgcttt attcaagata
    14281 atgagtctag ctcaagaaaa ggcgttttaa gaggaatgca ttttcaggtt aaatatcctc
    14341 aaggaaaatt agtcagagtt acagagggag aagttttcga tgttgcagtt gatttaagga
    14401 aagattcaca aacatttggg aaatgggaag gtgtaatatt gtctgataag aataaaaaac
    14461 aattttatat tccagaaggg tttgcacatg gatttttagt tttatcagaa aaagcagtat
    14521 ttagttacaa atgtacaaat tattattacc cagaatttga gaatggcttt atatggaatg
    14581 acaagcaaat atctattgag tggcctttaa aaggaattga agaaatattt ttatcagaaa
    14641 aggacaaaaa acaaaaaagt tttaaagata tttgtacaag acgttaataa aaatttatta
    14701 gaattaacat ttaggagaaa aaatatgaaa aaaatactga taactggagg agcaggattt
    14761 attgggagta attttataca ttatatgctt aacaagtatg tagattattt tattgttaac
    14821 ctagacttgt tgacttatgc tggtaattta gagacattaa tagaaataca agacaattcc
    14881 aattataaat ttgttaaagg agatatttca aatagggagc aggtgtataa gctatttgaa
    14941 gaagaaaggt ttgatatagt tgttaacttt gctgctgaat ctcatgtaga taggtcagtt
    15001 gagaatcctg atttatttat taaaacaaac attataggaa cacaagtact tttagatgca
    15061 tctataaaat atggagttaa aaggtatcat caaatatcta cagacgaagt gtatggtgat
    15121 ttaccaattg atagaaagga tttattcttt acagaacaat cttctatcaa tccaagttct
    15181 ccatattcag cttctaaagc atcagcagat ttattggtaa gttcttatta tagaacatat
    15241 ggattgttaa caactatatc taggtgttcc aacaattatg ggccatatca ttttcctgaa
    15301 aaattgattc ctcttatgat tttaaatgct ttggaaaaca aacaattgcc agtatatggg
    15361 aatggggaaa atgtaagaga ttggttacat gtatatgacc attgtactgc tatagactta
    15421 ataatacata aaggaaatat aggggagatt tacaatatag gaggtcataa tgaaagaagt
    15481 aatctagaag tagtaaaaat gatattaaat cttttaggta aatctgaaga gttaatatcc
    15541 tatgtaaatg atagacctgg tcatgattta agatatgcta ttgatgcaac taaaatagag
    15601 aatgagttag gttggaaggc aaaatatgac tttgatttgg gtataaaaga aactgttaag
    15661 tggtatatag aaaatgaatc ttggtggaaa gctgttttat cagtataact ttaaaattta
    15721 gaaaggggcg ttttatattg aatgagataa gttcactaat taatatatta gcattgactc
    15781 aattatctgg tagtaatact acaaatcaat gtaattgtac atataataat tcatctggat
    15841 ttgatatgat tatgatgacc ttgttaaaag cattatcaca aaataatcaa catacttcta
    15901 atagctacaa tttattgtct aattctgaga atttatttaa tgaacttgat aatgtagtaa
    15961 ataataccac tagtagattt atagatgttg atacaaaaga taaaaatgta aagttgagaa
    16021 ttgaaaatgc tgtagatcaa gcatcaaaaa aatataacgt agattcaaat cttataaaag
    16081 caataattaa agttgaatca gattttaatc caaataccgt ttcttctgct ggtgctaagg
    16141 gtcttatgca acttatgcca gaaaactgca gagacttagg agttacagac ccttttaata
    16201 tagaacagaa tatagatgct ggaactagac atattaagga atacattgat atgtttggag
    16261 ggagcataga aatgggattg atggcatata atggtggtcc tggaagaatg aaaagtagag
    16321 gagtagaatc aatctctgac ctgtataaaa tgccaaaaga aacacaaaat tatattccta
    16381 aggttatgaa atattataga gggtaggctt tattatggga ataaatatag ataagctaaa
    16441 cataagtcca agtataaata tggaggttaa aaaactaaaa gagagtaata aattttctga
    16501 caaattaaac tctttaaaac aatcttcttc attggaagat ataaaacgaa atttggaaag
    16561 tgtaaaaaag gcaggagaaa ggcttgtact tactcaaaac tatggcgata ttagtatata
    16621 taaaaatgca gtaaaagaat acctaaaatc tgttgtagat aatatgtatt cacttgataa
    16681 aaatagtagc ttttgggagc atcagtatta taagaatgta aagattgttg atagtaaact
    16741 tgaggatatg actagtaagt taattagtga agaaaaagaa aacatagata ttgtttctac
    16801 tgttgacatg atacaaggct tattgataga tatgtataga taaggttata caaggagaaa
    16861 aattttatga atagcaaaaa agaggatata tatgaattga attttgggca attaatagaa
    16921 gaaaaatctg gtgttataga agaaaatcaa aatgttttta atgcaaaagt aaatgtagct
    16981 gtttctatag gaaatacaac agaaacaata aaaaatatat tagatttaaa agaaggctct
    17041 ataataaaat taagtaagaa tgtggatgaa aaattagata tatatgcaaa cgatagattg
    17101 tttgcttatg gagaaagcat tattacaaat gagaggttat ctgtaagatt atctaaaata
    17161 gaagatccag atgtaaatta gcattttaaa tagtatccct ttaggctatt ctgtatgagt
    17221 ttagctaagg ggatattttt ataaaaaatt taaaatatgt gtgaattata taaatatttt
    17281 tattttatga gcgataacta aattataagt atgtaatttt ggaggtgaaa ctatggatat
    17341 taaaaatgta agttcaaata ttgtagtgaa taattattta aagaatacat ctggtataaa
    17401 aaaagtggaa gaggttaaaa aaattaataa aacagacaag atagagttat ctaaatttgc
    17461 aaaggaaatg gcaagtgtgt caaatatgag tgatgagaat attagaaata aaaaagtcga
    17521 tgaaataaaa aatgcattag aaaatggaac ttacaaaata aaaccaaaag aaattgcgaa
    17581 aaaaatgata gaggatatgc gaggataaag tcatgattac agacttaaaa gttataatat
    17641 atgaagaaaa aaatataatt agtaagatga tggaattatt aaaagaacaa tttgattata
    17701 tagttgaaaa agacttagaa aatttaaata tgttaaatcc taaactagaa gatattagca
    17761 gggagcttgc atccatagaa ataaagagaa gacaactttt tggtgatgat atttcaatga
    17821 gtgaagttgt tgaaaatagt aatgatggat acttaaaaga aatatataca gacttgaaac
    17881 acattcttgc tttagctaaa aatcaacaag aatcaaatga ttctctaata aagaaagaac
    17941 ttatttttac gaaaaaaatg attaatttca taaaaccagc tgataagcaa actacaactt
    18001 ataattctta tggaaatatt agaaaataaa tattaggagg agaaatatgg caggattatt
    18061 ttccatttta aatacagcta attcaggaat gaatgcgcac caaaaagcaa tacagacaat
    18121 ttctcataat ataagtaatc tagatacaga cggttattct aggcaaagga ccgagtttgc
    18181 cacaaatagt ccaatgtata tgcctagttt gagtaattca attgcaagag gacaattagg
    18241 gactggagta catgtaacgg atgtaacaag agctagaaat agtttttatg attaccaatt
    18301 tagggcagaa gctcataagt atggtaagac agtttcaaaa tatgattatt acaatactat
    18361 agaaactata ctaaatgaac catcagacta tggaatatct gcaggtatag atgatttttt
    18421 taaaggatgg gagtcattat caaaggatat aaatagtgac agtaaaaaaa gtctagtggt
    18481 agaaaacgca tctaatttag caactttaat aagtgaaagt tataagaagc ttgataagtt
    18541 aaaagaagat atagattcta atatagacac tgagataaaa caaataaatg acatgttaaa
    18601 aagtcttgaa gatttaaata aatctattga tattatatct ggttctggct ctacaccaaa
    18661 tgatttactt gatgaaagag atagaatttt agataacttg agttttaaac tagactttga
    18721 aaatagtgat gttaaaaata tgcttgcaga tggtaaatta gaacttaatg aattaaaaaa
    18781 tccagatgga acttggaaaa caggtatatc agggacctta caaggcttgt ttgaaatgca
    18841 tggtaagatt gatacatata aaagcgattt aaaagatgta tctgatggat tagctaaaca
    18901 gataaatgat gtttataata cgtctgcagg aataacagta acagattttt ttataacttc
    18961 aaatgtagat ggtgaagata ttataaagat aaatccagct ataaagagta acccaaatga
    19021 attaaagctt actgctgatg aagcttcaaa aattgctaag ttaaaggatg aaaaaataga
    19081 tattggggtg gctggtggaa aagtaagtac aataagtgac cactataaag cctttgcaga
    19141 atcaatcggt ttagattctc aaaaagtaaa tcaagatgaa gcaaatcaac gtaaaattat
    19201 aaataatatt gataactcaa gaatgagtgt ttcaggtgta tctttggatg aagaaatgac
    19261 agagcttata aaaatacaac gttcatatca agctagtgca aaagttatgt caacagctgt
    19321 gcaactattg gatgttgtaa taaatggaat aatttaatag aagggggaat ttataatgag
    19381 agtcagtaca ggtatgatgt ctagcagcta tctaaactca ctacaagata atctacaaag
    19441 attggataag gttaatagac aagttaatac aacaaaagaa ataaacaagc tatcagataa
    19501 tccatataaa gcaataaaga tattaaactc aaaaagtgaa ataaagacta tggagactta
    19561 tgttgagaat tgtaaagata cagggaattg gttagaaact actgatactg cattagatca
    19621 gcttggaaaa ttattatcag acataaagaa aactttggtg gcaagtggtg atggtgcata
    19681 ctcagatgat gaattaaaga ctcttagtaa tgaaactaat gaaaaaatga aagaaattgc
    19741 aaatgcttta aatgcaacac atgaagggaa gtacatattt tcaggttcaa atacgggggt
    19801 accacctgtt gaatgtatag ataatgctga tggttcagtg agtttaaagt ttaattctag
    19861 tctaaatctt aataaattaa atgactcttt gagtatggat atagctcaag gaataagtgt
    19921 agattataac gttaaattga gtagccttgg atttgaccca acaaaaactg acccatttga
    19981 agagttaaat aacatatcaa aaaaattagc aaatccagat gctgatgcta taaaagagct
    20041 tacaacaact tgtttgggcg atatggaaca actaatagaa aatacagtaa atgttaggtc
    20101 tatatatggg acaaaagcaa atacagtaga ggctatgaag gaaaaaaacg atgatagttt
    20161 gatacagtta aaggatattt tgtcacaaaa tgaagaaata gattatggtg aaaaacttgt
    20221 tcaattaaag gcagcagagt taacttatca agcttcacta caaacaggag gaaagttatt
    20281 taatgtatct atattagatt acatatagta aaataacgca gatatgatga aggttacatt
    20341 aaaaaaaggt atattaggtt ttgaaaattt aaaagaatat gagcttttag atatagaaga
    20401 taatgattct ttaaaagaat ttaattctac tgaagaagaa ggtataggat ttgttgttgt
    20461 atctccattt gagattatca atgaatatga aatagtttta aatcaggaaa caatagaaaa
    20521 actagaagtg aaatctccaa aggatgttgt actatttaat ataattactc ttggtcagac
    20581 atttgaagaa tcaactgtga acatgaaagc tcctatagtg ataaatataa aaactaattt
    20641 tggaatgcaa ataattttac aggatgataa atattcaata aggcatccat tattgagagg
    20701 tgataatgga tgctagtaat ttcaagaaaa aaagatgaag cagtacttat aggagaaagc
    20761 atagaagtaa aaattgtagg ggtagatgga aacaatgtga aactagctat atcggcgcct
    20821 aacaatataa gtatattaag aaaagaaata tacgaaaaag taaagagtga gaatataaaa
    20881 gctacaaata aaaatataaa aattttaaag tcattaaaat aataagcttg gaggaagtaa
    20941 tatatgtatg gagaaaatcc ttataattct tataaacaaa atgctgtttt tatggcatca
    21001 aaggaacaat tgttacttat gcttgttgat ggagcagtaa aatataccaa aatagccaga
    21061 gcagctatct tagataaaaa tacacagaaa gcgcatagag aacttgtaag agttcaggat
    21121 atatttacag aactaatggt tacattagac caaaatgcag gacaatgggc aaaggatatg
    21181 tacaaagttt atgattttgt aagatatgaa ctttcaagag ccaatattag aaaagacatc
    21241 caggtaatag atagtgtgtt gcctgtaata gaacaaatta aagatacatg gcatgaagct
    21301 gataaaaaaa gtaaagaaga aagaagtagg gacaaataat ggaattaaat ttagctgaat
    21361 tagataagtt atcaaaagag gaattactat tgatgcttgt agatggcaca gtaaaatata
    21421 ccaatatatc caaagaagct cttttaaata acgactattt gaaagcacat aatgaacttg
    21481 taagagttca aaatatattt acagaactaa tgactacatt agaccaaaat gcaggacaat
    21541 gggcaaagga tatgtataaa gtttatgaat ttataaaatc tgaattagca atagctgatg
    21601 aaaataaaga tattaaaata attgatgata tattgccaat agtaaaacaa attagagata
    21661 catggcatga agtttacaat caactaaaaa tataattaga tggagggata aaggtatgtc
    21721 aagtataagt cctataagag ttacaggtct ttcaggaaat tttgatatgg aaggcataat
    21781 cgaagctagt atgattagag acaaggaaaa agttgataaa gcaaaacaag aacaacaaat
    21841 cgttaaatgg aagcaagaaa tatatagaaa tgttatacaa gaatcaaaag atctttatga
    21901 taaatatcta agcgtaaatt ctcctaatag tatagtaagt gaaaaagcat actcttctac
    21961 aagaataacc agttctgatg aaagtattat agtagcaaaa ggttcagctg gtgcagaaaa
    22021 aataaattat caatttgcag tttctcaaat ggctgaacca gcaaaattta ctattaaact
    22081 aaattcaaat gaacctattg ttcgacaatt ccctccaaat gctagtggag ctagttcttt
    22141 aactatagga gatgtaaata taccaatatc tgaacaagat actacaagta ctattgtaag
    22201 taagataaac tcactttgcg cagataatga tataaaggct acttatagtg agatgacagg
    22261 tgaattgatt atttcaagaa aacagactgg ttcgtcatca gacattaatt taagagtaat
    22321 tggaaatgac aatttggctc agcaaattgc caatgataat ggtatcacat ttataaatga
    22381 tgctggtgga aacaaagtgg caagtgtata tggaaagaat ctagaagctg atgtaactga
    22441 tgaacatgga agagtaactc atataagtaa agaacaaaat tcatttaata tagataatat
    22501 tgactataat gtaaattcaa aaggaactgc aaagttgact tctatcactg atactgaaga
    22561 agctgttaaa aatatgcaag catttgtgga tgattataat aaactgatgg acaaggtcta
    22621 tggtttagtt actactaaaa agccaaaaga ttatccacct cttacagatg cccaaaaaga
    22681 agatatgaca actgaagaaa tagaaaaatg ggaaaagaaa gctaaagaag gtatacttag
    22741 aaatgatgat gagttaagag gttttgttga agatattcag tctgcatttt ttggagatgg
    22801 aaaaaatatt attgcattaa gaaaactagg tatcaatgaa agtgaaaatt acaataaaaa
    22861 aggtcaaata tcatttaatg cagatacttt ttcaaaggct cttatagatg atagtgataa
    22921 ggtatacaaa acactagcag gttattcttc gaattatgat gataagggaa tgtttgaaaa
    22981 gctaaaagat attgtatatg aatattctgg aagttcaact tctaaacttc ctaaaaaagc
    23041 aggtatagaa aaaactgctt ctgctagtga aaatgtatat tcaaaacaaa ttgcagagca
    23101 agaaagaaat ataagcaggt tagttgaaaa aatgaatgat aaagagaaaa gactttatgc
    23161 taaatattca gccttagaat ctttgttgaa tcagtattct tcccaaatga attatttctc
    23221 acaagcacag ggtaattaat tatggataaa ttaaaagaaa aattaaattt atataaagat
    23281 atatctttac aaattataaa tttgattgaa aaggaagaat atataaagat aagtattaag
    23341 ttaggtgaaa gacaagagat tataaatagt gtaagtgaaa ttgatagaaa tgattttata
    23401 cagctttata atagaatgga attaattgaa attgatagca ggataagaga tattttacaa
    23461 ggacaattat tagaagtaaa aaaagaatta catgagtata agcttactaa acaagtgaac
    23521 acaatgtatt ataacttaaa tagagaaaaa gtaaatattt ttaataaaaa agtataaagt
    23581 tatgtaccat cttgtccgat tatataaatg aagaattaat aaaaaagatt aaaagtagaa
    23641 atgacaagga tgtcaactat actaaggagg gtaaaataat gagagttaat acaaatgtaa
    23701 gtgctttgat agcaaataac caaatgggaa gaaacgtaaa tgcacaaagt aagtctatgg
    23761 agaagctatc ttctggtgtt agaattaaga gagctgctga cgatgctgct gggcttgcta
    23821 tatctgaaaa aatgagagct caaataaaag gtttagacca agcaggaaga aacgttcaag
    23881 atggtatatc tgttgtacaa acagcagaag gtgcattaga agaaactggt aacatactac
    23941 aaagaatgag aactttatca gtacaatctt caaatgaaac taatactgct gaagaaagac
    24001 aaaagatagc tgatgagtta ctacaattaa aagatgaagt tgaaagaata tcaagctcaa
    24061 ttgagtttaa tggtaagaaa ttacttgatg gaagttctac agaaataaga ttacaagttg
    24121 gagcaaactt tggaacaaat gttgcaggaa catctaacaa caacaatgaa ataaaagttg
    24181 ctctagtaaa tacttcaagt ataatgagta aagcaggtat aacaagttct acaatagcaa
    24241 gcttaaatgc agatggaact tctggaacaa atgcagctaa acaaatggta tctagcttag
    24301 atgtagctct taaagaatta aatacatcaa gagcaaaatt aggggcacaa caaaatagac
    24361 tagaatcaac tcaaaacaac ttaaataata ctatagaaaa tgttacagca gctgaatcaa
    24421 gaataagaga tacagatgtt gcttcagaaa tggttaactt atctaaaatg aatatattag
    24481 ttcaagcatc acagtcaatg cttgctcaag ctaatcaaca accacaagga gttttacaat
    24541 tattaggata aaataatttt gtatattgga tatagaagaa agggtaaggc tttgctttac
    24601 tcctttcttt ttttacaaat atacttttga aaggaaaatt tatatgttac ttagtatagt
    24661 tatgattgtt aaaaatgaag aaaaaatact tgaaaaaact ttaaaatcat taaccacatt
    24721 aagaaattct atagaaagtg aacttataat agtcgataca ggttctacag atgatactat
    24781 aaaaatatct aagaagtaca cggaaaaagt gtattttcat aattggaata atgatttttc
    24841 tagtatgaga aatatatcaa tatcctatgc taaaggtgaa tggttactta tattagatgc
    24901 agatgaaata ctaattgatt cttcaactat tgtaaaattt tttaaagatg gtcttgataa
    24961 aaaatttaat tctgcttcaa tacaattaaa aaatctttac tcatatgata aaaaattata
    25021 tggatattgt tcagtgctta gactttttaa aaacgttgat tttagatacc atggtaaagt
    25081 acatgaacaa ccaatataca aaaaccctat atttaataat gttgctgatt ttgaacatta
    25141 tggatatttg tttgaagatg aagaaataag attaaataag gtgaaaagaa atgaaaaact
    25201 attatttcaa gagttaaaag agaatgataa ttctccatat acaaactatc aattagggaa
    25261 aaattttatt atattaggaa aatatcagga ggctttatac tatcttgaga aatcttatga
    25321 attatattct aaactagaat tggcacctgg ttacttgata acaagccttg taaaaacata
    25381 tttatattta ggaaaacata agaagtgtga aagattgtgc ttaaagtata ttaagaaaga
    25441 catgaataat attgatatat attattatat tgcacaagca caagtagatt taggaaagta
    25501 tgaaaatagt atagattcgt ataagagata tatttacttg ttagataatt atgaaataag
    25561 tacacaagca aatagtttat tttcagacac tgatactgta gggcttagag atgaggctat
    25621 tattacactg ataaagattt attataagct tgaaaaatat gagtttgtaa tttcagagta
    25681 caataacatt gaagatgtgg aaaagaaaaa gaatgtgtac tttagcttat ttatgtccct
    25741 atataaattg aatagatttg aagatataaa aaattattat aaagaagttt caaattctaa
    25801 aattgaaaaa aatagttttt atagaaatat agaagaagtt ataaataata ttaaagagga
    25861 tgaaaaagag tatgtatata aaatcctttg tgacattgaa ggtaattatg gcattttaaa
    25921 tcaaataagg ataaataaat gtgtatctat agataaatgt aaagaaatac taaatgaaga
    25981 aaaggaaata atttatgcat atattataaa aatagctttt gaagagaaga ttgatttact
    26041 agatattttt tatgaaatgg aatatgtatg gatagagaaa tatctaaaat atcttttagc
    26101 attagaaaga agttttaatt tagtactata taagtacata attagtcgtc caaatacatc
    26161 agatatggaa aagataagag tatataaagc tatatctaaa gtagttttag aaagtataag
    26221 cttaagtgat gaaaaatata aggagatttt ttatttttat ataatgtatt cttataagta
    26281 tataagatac atatatagca atttaagtga ttttgaatta aaaaaatttg tttgcaatga
    26341 tttagataag ttcactatag aatttaagca tctacttgac attaaaaata aagtaaataa
    26401 tgctaatgaa aaattagagt atatacatag tatgaggaaa ttactaaatg agtatccttt
    26461 ttataataaa ataataaaaa tacttatagg tgaattagaa gaaaatataa aagaaagtga
    26521 agaatttaag gatttaaaat atagttttat tattagtata gaaaatatga ttgaaactgg
    26581 caaaataaat gatgcgaaat tattggtaga tgactattca aaaacatttg tggatgatgt
    26641 aaggatttta aatataaagg gaattttata tatgtttgaa aataagcttg ataaagctga
    26701 ttttatgtta aaaaaggcat tatcattaga tgtagaaaat gaagatacta tatataatat
    26761 agaatactta aaaagtttaa gataagtcaa aaataggaga attgaagaat gaatatacca
    26821 atagtggtta atgaaaaatc taaagacata ataaaagatt ggcaatatac atgtacaaat
    26881 gaagatataa aatttacaag cataataata ttgacatata atcaaataga atatactaaa
    26941 ttatgtattg aaagtattag gaaatttacc ccaaagaata aatatgaaat aataataatt
    27001 gataataact cttctgatgg aacagttgag tggttaaagg gacaatctga tataaaatct
    27061 gtgtataata acaaaaactt aggatttcca aaaggatgca atcaaggtat tgagttagct
    27121 actggtgaaa acatattgtt gttaaataat gatgtaatag ttactccaaa ttggctctat
    27181 aatttgaata aagctttatg gtctcaagat aatataggtg ctgtaggacc tattacgaat
    27241 gcatgttcat attatcagca aattgatgtt gattataagt caattgagga catgtttata
    27301 tttgcacaca aaataaataa atctaatatt aacctatgga aatatagatt gaagttagtt
    27361 ggattttgca tgttaataaa aaaaactgtt ctggataatg taggggtttt agatgaatta
    27421 ttcacacctg gaaattttga agatgatgat ttatctttta ggatgataga gtcaggatat
    27481 aagttattat tatgccagga tacttttata caccattttg gaagtgtgtc ttttaaagaa
    27541 aatcgtaata aatatacaga tataatgtat attaatagaa gaaaatttga aactaagtgg
    27601 ggatttaatc cagaatatag tggaatgata agaacagatt tgatttctaa aatagatgat
    27661 agtttggagg caaatattaa tgttttagaa ataggatgcg gtataggggc tactctttta
    27721 gaaataaaaa atatatataa aaatgcatca atatatggaa tagaaattag tgagtcagca
    27781 ggtaaacttg caaagtatag tgcagatgta cttatagcaa atatagaaaa agtaaaattg
    27841 gattataaaa aagatttttt tgattatata atattgggag atgtacttga acatttgatt
    27901 gacccatgga agatagtcaa tgaattgaaa caatatctaa agaaggatgg ttatctggta
    27961 tctagtatac caaatattat gcatgtttct gttttaagaa atcttgtgtt aggtaatttt
    28021 acatatgaag attcaggaat tttggacaag actcatttaa ggttttttac attaaatgag
    28081 ataacaaaaa tgtttaatga tagtaattac ataattaata gtattacagc tactagtatg
    28141 ccactttcgg aaattgataa tagcttcatt gatacaatat gtaaggaaag ctgtaaagaa
    28201 atgagacaac agtacgaatc ttatcaatat atagtaaaag cctctaaaat cctagatact
    28261 aaaagatatt ctagtattag tatgattaat ttaaaatacg ctttaatgag aattgataat
    28321 gaattagatg tagatagtaa tttaagttat atatttgata attataatat aactgaacca
    28381 tgttttgttg atgatattga atatctaatt gatacatttg tgataaataa agcaactcta
    28441 ttaaatagat taggagttga ggcatttaac agaggtctag gagattttag tataaacatg
    28501 tttatgacag tattagagat ggatagagat gatattgact caatatataa tataacaagt
    28561 gtattatgtg aattagaaga atttgatact gcttataatt taattaaaaa tagtagcgaa
    28621 aaagtacaga gtgaaagtga tatacaagag atactttgtt ttattgaggg gaagaaatat
    28681 gaataatgat aagatatgtt ttataacctg tgtaaatgat gaaaggcttt atgaagaatg
    28741 tcttgtatat ataaatagcc tagacattcc taaaaacttt agtatagaaa cagtagcttt
    28801 aagaggagcc gattctattg cctctgctta taataaagct atgagagaaa cagattctaa
    28861 gtataaggta tacttacatc aagatacttt tataattaat aaaaatttca tacatgatat
    28921 tttagagata tttaaaaaaa atacagatgt cggaatgatt ggtgtagctg gagtaaaaga
    28981 tatgcctgtg aatggaattt ggtgggattc tgataaaaaa gcagggaaag tatatgatag
    29041 tcatacaggt aaaatggata tttatgaatt tagtaacttt gaagaatgtt ttaatgaagt
    29101 tatggctata gatggattaa ttatgattac tcaatatgat gtattttgga gagaggataa
    29161 gtttgatggg tggcattttt atgatttgtc acagagtatg gagtttagac aaaataaata
    29221 caaagttatt gtaccaaaac aaaataagcc ttggtgtatg catgattctg gagtagtaaa
    29281 tataaataat ggatatgatg aatataggat aaaatttgta aaagagtaca taaatcttga
    29341 tttatataga gaaaattttt atgaatttga taagaagaaa gcaagtacca tacttaagag
    29401 taataagcat gtaatggatt gtatgaaaga ggagcttaca gaacaattaa gattaaagaa
    29461 ttacaaggat gttttaggta ttgtttctaa aattgctcat ttttctgttt ttaatcatac
    29521 tggtttgtat tcttgtgaag aatgtgagag agcattgcta gaatgtgcaa ataatttacc
    29581 agaaatagaa aatacgaaat tatttacaaa aaaagattta aaaagaagaa aagtattaca
    29641 tgttttaagt gaaggatatt caacaggagg tcatacaaga ctagttaaaa attggattga
    29701 aattgacaaa gactctgttc attcagttgt aactacatgg caatcagata cactgccaga
    29761 ctggttatta aaagcaataa atgatagtgg tggcgaagtt atatctttgg atttaatttc
    29821 agatgattat ttaaaaagag ctgtagcttt gaggaaaata gcttatgact gggctgatat
    29881 agttatttta tatgttcata tgtgggatac aatacctgtt gttgcttttg gagttgatgg
    29941 gaaaatacct attttacatg ttaatcacta tgatagtact ttttggttag ggacaagtat
    30001 tgcagatatt gtcattgatt ttagcgaaac tagtcaatta attacattag ataaaagagg
    30061 catagataaa tctgaaatat taccaatgcc attaaaagaa aaagaagtat tttcaaaatc
    30121 tgaagttaga aaacgatata atttagagga tgatgtgaca ataatattga ctattgctag
    30181 tgaaagaaaa tacaatagtg taaatgatat taattacttt aaaattgcta aaacattgat
    30241 tgaaaataca gataatacaa ttattattgt tgtaggagtt gatacagaga atctctattg
    30301 gaaaacttta agtaatgtta cagataatag agttatatgt tttggaacaa cagcaaatat
    30361 agatgatttt tataagattt cggatatata cctagagtct ttcatggtag gaagtcatac
    30421 ttcaaaatta gatgcaattt tatatgattt aattcctgtt aagtttaaaa atgaagtttc
    30481 accaattttt acaaatatgt ggaattttat agataagttt gattataaga atataaaaga
    30541 ggttatagac atgataagaa ggtacaatac tagtgaattt gacagggata agataaaagc
    30601 aaaacaatcg gaaataaaac attttgttat agataaacac tgtattgaaa taaaaaacta
    30661 tatagacaaa atatataatg aagttaatca tcataaagtt aagtatgatt tatataaaaa
    30721 tgatgaaatc gaggactatc agctttttgt agcattatat ggctatatgg taaaaaataa
    30781 aaacatgtca taggtggtaa aatgaacaat attcatatca ataaatttga aaaaagctgt
    30841 gaaaaaaact gggattattt tgtagaaaat aaatctataa atggtacatt tctgcaaact
    30901 agaaatttcc ttaattatca tgaggaggga aaattcactg accactcatt aataatatgt
    30961 aaaggtgaag aaatactagc agtcgtacct gcatgtgaaa cttatgaaga tggtgaaaga
    31021 atattttttt ctcatttagg gagtactttt ggtggcatta taataagaag tggttttaat
    31081 gatattaggc atgtaaatgc cattacaaat aagttagaaa tatatttaca ggaacaaaat
    31141 ttcgataaag tatatttaaa aaatacatca agaatatttt gtaaagagga tacttcatta
    31201 ctagaatatt ttttatttaa gaataattac atgttttatg atgaattaag tatgttttta
    31261 gaattaagta agtataaaga agatatatta gccaatttta aatacagtaa aagaagagat
    31321 tttaaatatt ctttgaagaa taatcttacc tttaaaagac tgtatagtga tgaagatata
    31381 gtttgttttt ttaatttact ttctaattgt ttagatagta aaggagctaa agtaatacat
    31441 tcagtagaag aacttttgga gtttaaaaat aatagattaa aagatattgt agaattttat
    31501 gcagtatata ttgatgacat tatgatttgt gggagcatgg tatttaactt tcaagaaaga
    31561 gtttatcata ctcaatatct agcatcaaat tcgacatata ataaatatta tccaatgata
    31621 tttttatact ataatctaat aaaagatgct ttagagagaa attttaagta tctttctttt
    31681 ggaattagca ctctaaatag aggcaaatac ttagatgagg gtttagctac atttaaagaa
    31741 ggttttggtt caagtgcaat ttgtaacaga acatactata aaaatataaa taagtgagaa
    31801 tcatgtttta tagagttttt agagtatagt aaatatctag tactataata ttacataaaa
    31861 ataattagtt tataaagaag gtttgtatat gaaaagatta atgattttag gctcaattaa
    31921 gtattttgag aatattgtta tgtcagctaa aagagaggga atatacacaa ttgtttgtga
    31981 taatagaatt gatactcctg cgaaaaagat ttgtgatgaa gcaatagatg ttgatgtatt
    32041 tgattttgat aaaataaaga atatagctat agataaaaag atagatggaa ttttaactgg
    32101 atttacagat tctttaatga agccatatgt ttatattgca aatgaactaa atttgccatg
    32161 tgtgatttct tgcaatcaac ttgaatctgt aactaataag gctgttatga aaaattattt
    32221 taaaaataat ggaataccta caactgatta ttgtattatt gaaagtttga aagatttaga
    32281 aaaaatacaa ggattaaatt ttccacttgt aataaaacct gttgatggtt atggttcaag
    32341 aggtgtatat tttgtatcaa acatagaaga attaatagaa aaatttgaat attctaagga
    32401 agcctcgcaa gatggacaag taataattga ggaattttat gaaagtgagg aaatacaagg
    32461 actagcttgg gttcataatg gagagtcaca tgtattttat attggagata gagagcttgt
    32521 aaatatacat acaggaagag ctggaaaacc agatagatta atttatccat caaagtattg
    32581 ttttcaatat gaagaagaaa taaaatccat atatcaaaat ataacgcagg cttttggcat
    32641 aaaaaatgga cctctttatg tacaaatgtt agtgggagca tatggaataa aggtatctga
    32701 agtaatgcca agattacctg gaggttgtga ctatatggct atttctcaaa taacagattt
    32761 agacatagga agtttatttg caaactttag tgtgaataat gaagtaaatt ttgatgaaat
    32821 aaagaaatat aatatgcatc tatcaaaatg tgtatatgca ttaccaatat acataagacc
    32881 tggcacaata aaaaagataa ataatataga taaaatagaa aacttagatt atgtcactca
    32941 atttctatta ctagtaaaag aaggagatac aataggatta tatgaagatg agagacagga
    33001 ttgtggaaga gtttatggag tagcaaataa tgtatttgat gcaaatataa agaagaaatg
    33061 tattcatgaa atgatagaaa tacttgatga aaataatgaa aatatgatag aaaattttta
    33121 aataagggag tatgatagat atgaagctaa taactcatga acaaattatg tcattaaaca
    33181 tttctcagaa acaaggatac gagtggataa aagaatcctt tttaacaaaa aaagattgtc
    33241 aattaccacc aaaaataagc atggtagaag aagggcatgt attttacaat gtaatgccgt
    33301 ccctaaatca taatcataag attggtggaa taaaaatggt tacaagattg ccagggagaa
    33361 atccaagttt aaattcagag atatttctat ataatatgga agatgctaaa ttaaaggcta
    33421 ttttagatgg caattatatt acagctttta ggactgctgc tgttgctgcc ttggctatcg
    33481 atacatttgc taaaaaagat tttaatacta ttgctattat gggtttaggt gtaactgctt
    33541 ctgctataat gaatatgctt tcatatactc ttaaagataa aaatataaat ataaagttac
    33601 ttaaatataa agaccaagca gagtcgtttg taaaaaagta tgaaaaaaat gaacaattta
    33661 gttttgaaat atgtgacaca catgaagaac ttattgtaga ttcagatgta gttatttcta
    33721 ctgtgacata tacagatgaa tatttatcag aatttaaatg gtttaaaaaa ggtgctttat
    33781 tattacctgt acatgttatg ggatttggaa atgttgatta tctatttgat aaagtatatt
    33841 tggatgatta taatcatttg aaacatctag aaaatataga tagatataaa tattcttgtg
    33901 aattttatga tgttttaagt aaacaagcta aaggaagaga aaatgatgat gagagaataa
    33961 tatcttacaa tataggtttg tcaatacatg atatattatt tgcaaataaa atttacgaat
    34021 tgtgtgaaac actaagctgt gaagaaatag aattaaaaga accaaaagaa aaatattgga
    34081 tatagtttat ttcattcata tttacaatag tttattgtaa atatgaatat gaagtaatat
    34141 attgttagaa aattagcatg aataggatag ataaatttat ggataaatat aatttaatta
    34201 ggttcgtatc tattggaaac gaatatatag gaaatttagt tgcattagaa tcaaataaag
    34261 caataccttt tgatataaag agagtttatt acatatatga tgtaccaaac aatgtaaaga
    34321 gaggatttca tgcacacaaa aacttggaac aagtactagt ttgcctaaat ggaagtgtga
    34381 agataaaatg ttatgatggt gtaaaagaga ctatatttaa tttacataaa aatgatgttg
    34441 gattatatat aggaacggga gtgtggcatg agatgtttga ttttgatgaa aaaactgttt
    34501 taatttctat agcatctgaa cattataatg aagaagatta tattagagac tataaagaat
    34561 ttttaaagta tttaaataat gtgtagttat tatacaatta gtgatgtatt ttcttaatta
    34621 aataaattta agatagccac atagagaata gatagattag gatgtgaatt tattgttggt
    34681 taaatttaat aatttattaa aacagttcca aactcataaa gaagaatatg aacagaagat
    34741 taaggatgta atggaaagtg gatattatat ccttggaaat gaagtaaagc attttgaaga
    34801 agaatttgct aactatgtag gtagcaaata ttgtgttggt ttagccagtg gtttagatgc
    34861 actgataatt gcttttagag cattaggaat aagcaaaggt gatgaagtaa tcgtgcaatc
    34921 aaatacatat attgctagtg ttatgggaat cagtataaat ggagcaacac cagtatttgt
    34981 agaaccaaat gaatattata atatagattc atctaagata gaagaaaaga taaatagcaa
    35041 aacaaaagct atattagtag tgcatttata tggtcaagca agtaatatga aagatataca
    35101 agaattatgt gataagtata acttgtattt aattgaagat tgtgctcaat ctcatggagc
    35161 taaatatgat ggaaaaatga caggaacatt tggtgatata ggttgtttta gtttttatcc
    35221 aagtaaaaat ttgggtgcat ttggagatgc aggagctata gtaactagta atgaggaaat
    35281 agctgataaa gtgaaaattc ttagaaacta tggctcagaa aagcgatatt ataataaatt
    35341 tgtcggatat aattcaagat tggacgaaat tcaggcaggt ttattaagag taaaacttaa
    35401 atatctaaat gaaattgaaa ttgaaaaatc aaaaatcgta gatagatatc ttaatgaaat
    35461 taataatccc aaagtcatac ttccaaagat agaaaataat tgtactcatg tatggcattt
    35521 atttgtttta caagttgaaa aacgagatac atttatggaa tacttaaaga aaagtggtat
    35581 agagtctata atacattatc cgataccacc acatctatct gaagcatatg cttatttggg
    35641 atataaaaag gggagtttta atatagctga gaactattca gataaagttg ttagtatacc
    35701 tttatttatt ggtatggaag atgaagaagt tagttatata attaaaatta taaatgaata
    35761 t
//
LOCUS       DAEOSP010000019        35164 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.019, whole genome shotgun sequence.
ACCESSION   DAEOSP010000019 DAEOSP010000000
VERSION     DAEOSP010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35164
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.019"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            31..420
                     /locus_tag="KRH54_000732"
     CDS             31..420
                     /locus_tag="KRH54_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8578404.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            413..1504
                     /locus_tag="KRH54_000733"
     CDS             413..1504
                     /locus_tag="KRH54_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBG8578405.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            1624..2100
                     /locus_tag="KRH54_000734"
     CDS             1624..2100
                     /locus_tag="KRH54_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG8578406.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            2486..2953
                     /locus_tag="KRH54_000735"
     CDS             2486..2953
                     /locus_tag="KRH54_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG8578407.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(3013..3585)
                     /locus_tag="KRH54_000736"
     CDS             complement(3013..3585)
                     /locus_tag="KRH54_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG8578408.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGTPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(3648..4745)
                     /locus_tag="KRH54_000737"
     CDS             complement(3648..4745)
                     /locus_tag="KRH54_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG8578409.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            complement(4759..5448)
                     /locus_tag="KRH54_000738"
     CDS             complement(4759..5448)
                     /locus_tag="KRH54_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG8578410.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            complement(5613..6779)
                     /gene="rpoD"
                     /locus_tag="KRH54_000739"
     CDS             complement(5613..6779)
                     /gene="rpoD"
                     /locus_tag="KRH54_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBG8578411.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            complement(6797..8587)
                     /locus_tag="KRH54_000740"
     CDS             complement(6797..8587)
                     /locus_tag="KRH54_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBG8578412.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            complement(8842..9627)
                     /gene="aroF"
                     /locus_tag="KRH54_000741"
     CDS             complement(8842..9627)
                     /gene="aroF"
                     /locus_tag="KRH54_000741"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG8578413.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            complement(9608..10114)
                     /gene="folK"
                     /locus_tag="KRH54_000742"
     CDS             complement(9608..10114)
                     /gene="folK"
                     /locus_tag="KRH54_000742"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBG8578414.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            complement(10107..10472)
                     /gene="folB"
                     /locus_tag="KRH54_000743"
     CDS             complement(10107..10472)
                     /gene="folB"
                     /locus_tag="KRH54_000743"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBG8578415.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            complement(10483..11274)
                     /gene="folP"
                     /locus_tag="KRH54_000744"
     CDS             complement(10483..11274)
                     /gene="folP"
                     /locus_tag="KRH54_000744"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBG8578416.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            complement(11338..11910)
                     /gene="folE"
                     /locus_tag="KRH54_000745"
     CDS             complement(11338..11910)
                     /gene="folE"
                     /locus_tag="KRH54_000745"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBG8578417.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            complement(11936..12637)
                     /locus_tag="KRH54_000746"
     CDS             complement(11936..12637)
                     /locus_tag="KRH54_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578418.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVVIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKILNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            complement(12671..13414)
                     /locus_tag="KRH54_000747"
     CDS             complement(12671..13414)
                     /locus_tag="KRH54_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HBG8578419.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            complement(13404..14753)
                     /gene="pabB"
                     /locus_tag="KRH54_000748"
     CDS             complement(13404..14753)
                     /gene="pabB"
                     /locus_tag="KRH54_000748"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBG8578420.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            complement(14755..15339)
                     /locus_tag="KRH54_000749"
     CDS             complement(14755..15339)
                     /locus_tag="KRH54_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBG8578421.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            complement(15363..16082)
                     /locus_tag="KRH54_000750"
     CDS             complement(15363..16082)
                     /locus_tag="KRH54_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG8578422.1"
                     /translation="MTSWKKKTVYKCLIAVALFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            complement(16110..17174)
                     /locus_tag="KRH54_000751"
     CDS             complement(16110..17174)
                     /locus_tag="KRH54_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBG8578423.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            complement(17318..18127)
                     /locus_tag="KRH54_000752"
     CDS             complement(17318..18127)
                     /locus_tag="KRH54_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578424.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            complement(18369..19235)
                     /locus_tag="KRH54_000753"
     CDS             complement(18369..19235)
                     /locus_tag="KRH54_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG8578425.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            complement(19755..20444)
                     /locus_tag="KRH54_000754"
     CDS             complement(19755..20444)
                     /locus_tag="KRH54_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBG8578426.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGVESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            complement(20437..20808)
                     /locus_tag="KRH54_000755"
     CDS             complement(20437..20808)
                     /locus_tag="KRH54_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBG8578427.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            complement(20990..22336)
                     /locus_tag="KRH54_000756"
     CDS             complement(20990..22336)
                     /locus_tag="KRH54_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG8578428.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            complement(22518..23111)
                     /locus_tag="KRH54_000757"
     CDS             complement(22518..23111)
                     /locus_tag="KRH54_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBG8578429.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            complement(23176..24645)
                     /locus_tag="KRH54_000758"
     CDS             complement(23176..24645)
                     /locus_tag="KRH54_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG8578430.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            complement(24760..26151)
                     /locus_tag="KRH54_000759"
     CDS             complement(24760..26151)
                     /locus_tag="KRH54_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBG8578431.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            complement(26317..26565)
                     /locus_tag="KRH54_000760"
     CDS             complement(26317..26565)
                     /locus_tag="KRH54_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578432.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            26831..28960
                     /locus_tag="KRH54_000761"
     CDS             26831..28960
                     /locus_tag="KRH54_000761"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBG8578433.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            complement(29217..29816)
                     /locus_tag="KRH54_000762"
     CDS             complement(29217..29816)
                     /locus_tag="KRH54_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBG8578434.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(29834..32953)
                     /locus_tag="KRH54_000763"
     CDS             complement(29834..32953)
                     /locus_tag="KRH54_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG8578435.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPVEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FLEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKYRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            33174..33998
                     /gene="pdaA"
                     /locus_tag="KRH54_000764"
     CDS             33174..33998
                     /gene="pdaA"
                     /locus_tag="KRH54_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG8578436.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            complement(34095..34880)
                     /locus_tag="KRH54_000765"
     CDS             complement(34095..34880)
                     /locus_tag="KRH54_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8578437.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
BASE COUNT        11237 a         6052 c         3584 g        14291 t
ORIGIN      
        1 ttcatttata aagcataatg gaggtttttt atggttttaa atttagattt taatagtgat
       61 aaatctattt atgtacaaat taaagaagaa ataactaagg ctatagcttc aggagaattg
      121 aaaataaatg aatcacttcc atctgtaaga agtatggctg aaaatattgg tgtaaattta
      181 catacagtga acaaatccta caatgaatta aaagaagagg gattcttaaa tattgataga
      241 agaaaaggtg ctattgtagc tcaactacca atgaaaatag ataatacaac tcgtcaaaaa
      301 aataaacttg acttggaatt attggtggca aaatcttatt tatcaggtat tagtaaagaa
      361 gaatttttgt cactaagttc aagtttattt gacaagtatg aggtgaaata ttatgagtaa
      421 gattgaatgt ctatttacaa tatttccagt tctaattttg atgtatgcta cattttattt
      481 tatgccctat tttgtaggta aaaaacacat ctatggagtt tctatagacc aagagcataa
      541 aaattataat tactttataa agttagataa agaatttaaa aaactattat ctttaggttt
      601 tattatagac tttatattag tttttattct ggtctttaca tttaataaat tggaactttc
      661 ctattttatt ggtattatag gttttttatt atatgaaagt atactatata ttcatacaca
      721 taaaaaagct aagaagctta aatcagaaat ttattctaaa ttaggtcata tagatataga
      781 ttcaaaatta atagtagata tggattttat aaataagaaa aataaaataa taaaaaaatt
      841 taaaataatc tatttaatac cgatactatt tactttggga atgagtattt ttatagcatt
      901 taattataat caattgccag actcaattgc cactcattgg aacataaatg ggtctcctga
      961 tgtttttata gataaatcat ttcttaatgt atttaaatta atcggtactg acttttgttt
     1021 aatggtttta ttatatataa cttccattgg atccataaag tcaaggatta agatagatac
     1081 aaatagaatt gaagaaagta gagttgaaaa cattaaatat ttaaataaaa ttggttattt
     1141 gtttttaata ttgatgatta taatgacaac acagtttttt actactcttt tatcaataaa
     1201 aacaaaacta tcaactgcta tgaatattac aacgctttta gttataattt atttaatagc
     1261 aacatatatt aattccccaa atttaaaatt caactcatct tattctccag aagaagatga
     1321 gaagtattgg atagcaggca tcatgtataa taatccaaat gacccatcat taatggttaa
     1381 taaaaggttt ggtattggat ggactataaa ttttggcaat cctcttggaa aaatactata
     1441 tattgctata gctttacttt taattttttc attgtttagc ctaataaaat ctctcttact
     1501 ttaaaatacc ataaaaggta tcttacttgt attattgggt attatatttt gtaggataag
     1561 aaatatttca tattatcgtt gaagtatagt atttatatat tactaagaat ggagattttc
     1621 ataatgttgt caaaagctga aatcaaatat tttaatgaat gtgcttctga gatactttct
     1681 tctgaaaaag ttcaacttat gagaacattt cctcatcatg gcaatgtttc atgtcttgaa
     1741 catagtttgt cagtagcata ttatagttat ctattatgta aaaaattgca cttaagtgtt
     1801 gatattcaaa gtgttattag aggagcttta ctacacgact tttttcttta tgattggcat
     1861 tacaaaggtg atagaaaagg attacatgga tttactcacc ctagagaagc attaaaaaat
     1921 gcaactttat tttttcaaat aaatgaaaaa gaaacggaca taattttaaa acacatgtgg
     1981 ccacttactg ttaagccacc tagatacaaa gaagcattta ttgtatgttt gttggataaa
     2041 ttttgctgtt tagttgagac tttaaagata cattctttat tatctcctta ccatgtatag
     2101 gacaattccc acaacacttt tataacttaa ttttgaagag gtattaagta ttatattcaa
     2161 cttgatacct ctttactatt tattatttta tataataata ttttctttat aatatagtta
     2221 taaataacaa cttctaccct cgataacttc cccttcttat agacatgctt actatcttaa
     2281 ataacaagaa accagctatt aaatttatta gtattgttta tactactcta acaataatat
     2341 cataaataaa cctccaaata gcttctcttt cactaccttt tatcttaaac tatatttttt
     2401 tcttaaaatt tgtaaaaaac atagcataat ctttataaat gttctaaaat ttaatttaag
     2461 catcaaaatt agaaaggatg atattatgtt taaagaaatg agattgaaaa aaagagaaat
     2521 gacaaaagaa gatactgttg aagtattaaa aaatggtgaa tttggtactt tttccactat
     2581 atctgaaaat ggttatcctt atggagttgc tgtaaattat gtatacttta atgattctat
     2641 ttactttcat tgtgcaagaa atgggcataa gttagataat atatcgaaaa acaacaaagt
     2701 ttctttttta gtagttgcta atgaaagtgt tattccagat aaatttagta ctacttattc
     2761 tagtgctata gtttttggaa aagcttgcac tgtagaaaat gaagaaaaga aaaatgctct
     2821 tgtagaaata ataaaaaaat attctaaagg attctttgaa gaaggcatga agtacataga
     2881 gaaggatatg aacttaacaa ctgttgttaa aatagaaatt gaccatattt ctggtaaagc
     2941 ttcacgttta taattaagta aaaataccta aaagtatata cagtcgcttt taggtatttt
     3001 tatatgatat tcttatatgt taagtctttt taggtaatct cttataccta tcttggcatc
     3061 atcatttggt cttatatcag aattatctcc tgctatatga agtattccat acgctggata
     3121 acctcctctt actgaacatg gaactgacaa aagagtcatt cccatatgtt gggcaaatat
     3181 attcatagct tgcaaagcta attctcctcc tccatgttgt gaacctgctg tagcaaaaca
     3241 accaaatatt ttaccactca aaggagtacc tacccaaata tcagaaaaac tatccataaa
     3301 catcttcatt ggtccagata catttccata atatgttggt gaacccataa atatagcatc
     3361 ataatctaat atttcttttc cagatttaat tacatttata ctttcaaatt cgtctttgta
     3421 ttctttggaa tttatcaaat aatattgagg aagtgttttt gcaacttcgt cacttactct
     3481 aaatatatct acctcgatat tcatttcttc aagcgcttct ttatattctc tagctaataa
     3541 atatgtactc ccacaaacac tatgaaaaat tactgctact ttcatcatta tccccctaat
     3601 taaactcaaa tttatttttt atatagtatt tttattatca aacactgcta aatagtacta
     3661 aatggattca aatatacatt actttttatt acttcaattt cactaaattc atctaaaaat
     3721 ctcttcataa catttttgaa tatatcttct gtatcaaaat gaccacaatc cactatacac
     3781 attccaatat caagtgcatc ttgtgcctca tgatacttta catcacccgt aattagtact
     3841 tctgcacctt gtctcttagc tttttttaca aattctgaac cagctcctgt tacaacggct
     3901 actttcgtta tgtctgtact aaggtttcca actactctta tgtgtttcat atttaatttc
     3961 tcttttattt tccttgaaag actttctaat gtcatacttt tatcaagttt acttattcta
     4021 cctagtccaa atatctcacc tttattttct aacttgtaca aatcatatgc aacttcttca
     4081 taaggatgtg catctaacat agaacttatt accccaccta aaagcttctg tggtactaca
     4141 gtttcaattt taacttcatt tacagtttct atatcattta cacttccaag aaacggatta
     4201 gagccttcta atggcttaaa ttgtccttca ccttctattg aaaacgtaca atcactataa
     4261 ttccctatat gacctgctcc tgaattactt aaaacctttc taagttcatc tgaatagtta
     4321 tgtggaacgt atactgctaa tttatacaaa ttttcgcttt tagttatatc taaaaccttt
     4381 gaattaccaa attccataat ttccataaaa tagtcattta gaccatcaaa tgctatatca
     4441 aaattcgtat gcatagaata aagtgatata tcatttttaa taagtttttg gataagtctg
     4501 ccttttaaat ctccactatt aattttatta attttcccaa atataaaagg atgatgtgtc
     4561 acaattaaat ctatattttt ggatatagct tcatcaataa catcttcatt agcttctaaa
     4621 gataccaata ccttttttac atccatatca aaatctccaa ctataagacc tacattatcc
     4681 caatcctctg ctaagtttag aggatacttt ttttcaattt ttctagttag actctttaat
     4741 agcatatata cctcctattt attttattaa attttcaagt ttttttattg caacttcgct
     4801 ttcctttctc ttcttatcta tagcttcccc atttttccct tctaatttat taactataga
     4861 actatatgtc tttatctttt tttctataaa atcattaaaa agagaatctt tgttttctaa
     4921 aagctttatt ccaacctcat aatatatctc atcttctatt atagtatttt tacctgtata
     4981 tttggcaaca attatctcat atattctaaa atcttctctc actaaaactt cttctaaaat
     5041 ttcatatccg ttgtttaaaa ggtaatatct aagttcttct tgtgcttgca ttggctgtaa
     5101 tattaatttt tctacattat gtgctacttc ttttttagcc tcgagaagct cacttatcaa
     5161 gattccaccc ataccagcaa ttatcacttc ttctacttcc cctatttcaa gtatctctat
     5221 accagaaccc aatcgtaaat caactttatc taaaagattg ttctgtatga cttctttatg
     5281 tgcattgtca agaggtcctt tattaacatc cgctagcact gcaaatggta cttttccctc
     5341 ttttaacaaa tatactggta tgtatccgtg gtccgtccct atatcagcta tttttttacc
     5401 atctgacact aatgatgcta ttttcaataa tctatctgtt aatttcaaat ataattctcc
     5461 tttcaattcc ctttatttat aggatttaat ccaagttata gatattagac tgaaataact
     5521 acaaaaataa aattaaatct ttactacaaa atagccatga gatatcactt atctcatggc
     5581 atttatcaca aaaacatcaa tgatgtatct ttttagtcta aataatctct aagttttttt
     5641 gatctacttg gatgtcttag ttttcttagt gcttttgctt ctatctgtct tattctctct
     5701 ctagttacat caaactcttt tccaacttcc tcaagagttc tggctctacc atcctcaaga
     5761 ccaaatctaa gctttaatac tttttgttct ctatcattta atgaaccaag tacatcttct
     5821 atttgttctt ttaataaaga atatgctgct gcctctgctg gggctggagc atcatcatct
     5881 ggaataaagt cacctaaatg gctatcttct tcttctccta ttggtgtttc taaagacaca
     5941 gggtcttgag ctattttcat tatttctctt actttatctt ctgtcatttc catttctttt
     6001 gcaatttctt ctggttttgg atctcttcca agttcttgaa gtagttgtct tgatactctt
     6061 attagcttat ttatagtctc taccatatga actggtattc ttatagttct agcttggtct
     6121 gcaatagcac gtgttatagc ttgtcttatc caccatgttg cataagtact aaacttatat
     6181 ccttttgtat agtcaaattt ttcgactgct tttataagac ctaaatttcc ctcttgtatt
     6241 aaatccaaga aaagcatacc tcttcctaca tatctttttg ctatacttac aactagtctt
     6301 aagtttgctt caactaacct ttgttttgct atctcatcac cttcgtgcat ccttctagca
     6361 aattctactt cttcatgtgg tttaagtaga ggaatttttc ctatttcttt taagtacatt
     6421 cttacagggt catctatact tatccctttt ggtaaagata aatccacagt ttctatttct
     6481 attgcagaag agtcatcatg tgaaattgcc tctgcatctt catctaagtg gcctatactt
     6541 aaatcgtcat cagcaaccga aaaatctata tcagctttat agttttttgt ttctgttatt
     6601 tctattccca aatttcctaa agtctcataa agattttcta cttgatcctt atcaagttca
     6661 gtctccgaaa aagcttccat tatctctgca agtgtcagag aaccttgctt ttttcctttt
     6721 tctattaatg tcttagcagt aacctttttt aactcttttt tattcgattt attttccaca
     6781 ctcaaacctc ctttctctac aagctcttta aaattttatt tatctcaact atttttaaag
     6841 caatttccat aactctacca tctacctctt tcatatcatt attattttct aattcttgtt
     6901 gttctctcaa caagctatta atttgctctt ctaatgagtt ttttctgata tttgttatta
     6961 ttccttctat ttcttttgtg ttctctaagt ttatactatt caaagaaatt gaatttaatt
     7021 cttttaagta ttcttcggat atgtttaaac ttttcaattt gtcaatagtt attttgtcca
     7081 attcttgatt tttaatcatg taatttaaaa tttctttact ttctttcaat aaaaaatcac
     7141 tttcttctac tttaagtaac gctatttctc taattttttt gtcttctaac attattttta
     7201 ttagagtttc ttcaactaat tgttttccgt cttgtctgac ttcgactttt tcaattactt
     7261 tttcttctat tttcttttga tttttgttat tatatggctt attgtaattt ttaccataaa
     7321 cttctctctt tatagactcg acattaatat ctatttgatt gcttaaatac tttgtataaa
     7381 aatcaatttc aacaggactt gtcaattgtt ttataatttt agaagcttct tttgcgaact
     7441 tcactcgttc ttcatctttt tgaatgttaa attctttttt aagatgatct attttatatt
     7501 ttatgtagtg agtagaaaca tccatagctt ttttataatc actaagtccg tattttctta
     7561 caaattcatc tgggtcttta gcatctttta agtctaacac ttttacattg atacctaatt
     7621 tagtaagtat atctatagct cttaaagtcg cttttattcc tgcttcatca gaatcataag
     7681 atataatagc agtgtccgca tatctcttta ttaaaatgcc ttgttgttca gtcaaggctg
     7741 ttccaagggt tgctactaca ttttttatac catactgata aagcgaaatt aaatccatat
     7801 atccttcaac taagactata gttttacttt ctaaattttt tctagcaaaa tttaatccgt
     7861 ataagttctg ttttttatta aaaattaacg tgtctggaga atttaagtat tttggcaaag
     7921 aatcgtctaa tactcttccc ccaaatccta ttatatttcc tctataatcg aatataggaa
     7981 acataactct atttctaaat ttatcatagc aattagttcc atctctattt tttgcaatta
     8041 gaccgcactc caataagtct tgttttttat atcctttact gattagagta ttcataagtg
     8101 aattccatga atccaaagaa aatcctaaac caaatttctt aataatttta tcatctagtc
     8161 ctcttattct taaatattca tacccaagat ttttgcttcc caataagttt gaaagataaa
     8221 atctagctgc ttccgtatga atatcttgaa atttcttaga tttttctatt ttaatcctag
     8281 tttcttcatt catgttagtg ttaatttcta taccgcattt atttgctaaa atttttacag
     8341 catccataaa atccaaattt tccattttca tgacaaaatt tattacgtct ccaccttcac
     8401 cacaaccaaa acacttataa atctgttttg acgtatttat ataaaaagaa ggtgtctttt
     8461 cgccatgaaa tggacataat cctttgtaat tagctccaga ttgttttata ttcatatatt
     8521 cagatattat acttgctata tcgcatctag cttttatttc ttcaataatg tcttttatat
     8581 cattcatatt atcaccttct ggtgtaattt tttgtgttaa catttctatt ctacactata
     8641 ttctaaattt gtcaatcact caatatacac aattcgactt tttttattga tatccttctt
     8701 attaatcaaa aaatttttat tttttatatt aattctatca atattaaatc tttatttata
     8761 ttaaataatc taactatcaa attataactt ataaatttac cttagcaacg attacgaata
     8821 aaaaagaggc tcttgcctct tttaataatt attaattgat ttcattaaat cttcaaattc
     8881 attaaagcaa agagattgag caccatctga caaagcattt tctgggtcag gatgtacttc
     8941 taccattatt ccatctgctc cacatgcaaa agatgccagt gacataggtt ttaccaaata
     9001 tctaactcca gtggcatggc ttgggtctac cacaacagga agacctgttt ccttttgaat
     9061 aatgggtact gcagctaaat ctaatgtatt tctagtataa tcattatacg ttcttatacc
     9121 tctctcacac attataatat tactatttcc ttctattgct atatattcag aagcacctat
     9181 ccattcagta attgtggaag ctattcctct ttttaacata acaggtttat tttgttttcc
     9241 cacttcactt aaaagagtaa agttttgcat atttcttgac ccaatttgaa ttatatcact
     9301 tatactatac agttcgtcca tatctcttac atccataagt tctgtaataa cagccatacc
     9361 aacttcgtct ttaaccgctt ttaatatttc aagaccttct ttttttaatc cttgaaatga
     9421 atttggagat gttcttggct tgtatgctcc acctcttaaa atatttgctc cctgactttt
     9481 tacaaattta gcagtctcca aaagttgctc ataactctct attgcacatg gtccagctat
     9541 tataagttta tcttttttta cagttatctt tttgttattt gtatttatct ctatctttga
     9601 attattatta aataggtttt ctttcataac ctacaagctc ctttacttct tctctttctt
     9661 cagcagatag gaggtttaaa tgttctttta ttgttttttc atttattata agttcttcat
     9721 ttaaatccat aagtggtatc aaaacgaaag ctctatcttg aatccttgga tgaggtattg
     9781 ttagtctttc atcagtggaa actacatcat taaaaaatag tacgtctaca tctatagttc
     9841 ttggtcccca atgaactatt ctctctctgt gtagttctct ctctatttct tgacaatatt
     9901 ctaacaactc ataaggttca aactctgttt ctatttctac acacatattt agaaaatctg
     9961 cttgttctgt atatccccat ggttttgttt cgtataagct tgattctttt actttatata
    10021 tagaatcact atttttcaaa agttcacatg ctcttgataa attatcaaat ctatcaccca
    10081 tgttagtacc tattcccaaa taagccttat tcatctcttg ttctccttat ctcaacacca
    10141 aaatagtcat aaataccatt tacaggagcc tcaggctttc taactctgac cataactcca
    10201 tttataagta taaatttatt caagacttct tcagcaatat tttccgctaa agcctccaaa
    10261 agattaaatt gtttattttc agtaatatct ttaacaacat tgtaaacctc ggcatagctt
    10321 acagatttat ttatatcgtc actaagacct gcttctcttg aatcaatata aagttccatg
    10381 tcgacaaaga atttctgacc tagaaaattt tcttctttaa gaacaccatg atatccatag
    10441 aatcctagat tactaagtaa tattttatcc attatattat acctactttc tatatattgc
    10501 atcagtcatt ctagctgcca ttaaattttc ataaacatca tgtactctaa ctatatctac
    10561 gccatctctt ataccaagaa cagtagtagc tattgtacct tctaatcttt ttttaggctc
    10621 aactggaagt acttttccta ttactgattt tctggatgtc ccaagcaaaa taggataccc
    10681 taaatctttt aattcattta atcttctcaa tacttcaata ttttgttcaa aatccttgcc
    10741 aaatcctaca cctgggtcaa gtactatttt ttcttttttt acaccacaac tcattgccat
    10801 ttctatactt accttaaaaa agtcttttat tgactccatt atatctttgt catagtttgt
    10861 cccatcttga ttatgcatta tacacacttc tgcatcatat tttcctatta cagaagccat
    10921 atttttatct tttctaagac cccaaacatc atttaccata gtgacaccta attttaatgc
    10981 ttcttctgca acatctgcct tataagtgtc tattgatatt ggtatatcca attcctgttt
    11041 aagttttttt attacaggga ttactcttct tagctcttcg tctgagtcta catagctatg
    11101 acctgggcgt gtagactctc ctcctaggtc tattatatct gcaccttggt caaccatatc
    11161 ttttgcatgt tgtattgcta tgtccaagtt attaaaatct cctccatcag aaaaactatc
    11221 tggagttaca tttaatattc ccattatata agttctttta ccataatcaa acacaattaa
    11281 tgacactccc ttaaattaaa ttttatctca ataaatgatt atatctataa taaattacta
    11341 cttcatagta atcaaactca tagcctcggc tctagacgca atgtcctttt caaatattcc
    11401 tctcactgct gatgtaacag tttttgaacc tggttttttt acgcctctca ttgtcatgca
    11461 catatgttca gcttctacta ctactaagac accataaggt tgtaattctt ccattattat
    11521 atctgcagca tttttagtga ttctttcttg taattgtggt ctttttgcca atgtatctat
    11581 tactctagca agtttagaaa gccctgtaag tcttcctcct tttggtaaat aagcaatatg
    11641 agcttttcca aagaaaggaa ctagatgatg ctcacaacat gaataaaatg gaatatcttt
    11701 taccaatact aactcttcat gtttttcatc tgcaaaaaga acttttaaat ggtctcttgg
    11761 ttcttcacta agaccagaga atatctcttc atacatccta gcaactctat ttggagtttc
    11821 gattagacct tctctatctg ggtcttcccc aattgcctct agtatttctc ttactgcatg
    11881 ttgtatcttt tctttatcaa ctttattcat ttaaactccc cccattgatt tgtgactaga
    11941 agattagttt tataacctct tttgtctcaa tttctttaaa aatataatta taaacatcta
    12001 aaagtctttg atagtcataa ttttcactta cttttgacac tagttcaaaa agctcattat
    12061 tttctttttt taaaactttt agcaattttt tgtcttttgg tacccattta tcatttatat
    12121 taaagtaccc aattattata tcttttaaat ataaattcgt caaaaaatga tattcaaaaa
    12181 catcaaattt ttctttattt tttaaattca atatttttgc tccaatatct tgctttataa
    12241 aagacttttc ctctaatgac attttactag gtccttctaa gtaaatatct ctacttatat
    12301 cttttataat atgtgatata ttctccctat caaacagtac tttagcatct ttcatttctt
    12361 tcacaaagaa atattcccta tttgataata atttttttac accatctttt gaaaagtaat
    12421 ttacatcaaa ttctattccc ttttttttaa acagtattct ctcttgtttt tccccttctt
    12481 ttacaaaaac gaacacatca atatcgttta tcactacatc ataaagagta aaatctacat
    12541 ctttactaga acccactaaa tatacagcca aagtgttttg gttttctatt attttagtca
    12601 tctcttcatt gtatacttca taaatacaac ctatcatctt ttgtctccaa caaattgtta
    12661 atattttaat ttattctaat aacttcacta ttttatcaaa aacaacattt tctttattaa
    12721 attttatttt gtcaatttct gtaaccttta tagcgcccat aagactattt gtgacaaaaa
    12781 caaaatcaaa actactaatt tctgatatat taatttcatt ttctatcaac tctatatgta
    12841 attcgtcaca aatctctata atcctttttt ttattattcc atttaatatc ggtaatctat
    12901 cactaggagt gtacactctt tctcctttta taaagaaaat attgctcata gaacattcta
    12961 atattacacc ttctgaattt aaaaatatac catcattata accagtctta tttgcgatat
    13021 ttttggtata tatgttttca aaataattag tagtcttatg tctatgaatt aaactattcc
    13081 ccctaactat tggagatata gctaatttaa accccttctc atacgtctct tgattatatg
    13141 gaatatccct aatagatata ttataacctt catcaaaaac agtaattcta agtgctttat
    13201 taatgacatt ttcttttttt atatatgtaa caatttcatt tatcaagaaa tcttttttat
    13261 agtttatatc taaatccaaa caatttattg agcttagcat tctttctata tggatatcta
    13321 aatctactgc tattccattt ttaactctaa tagtttcaaa caaaccaatt ccaaatttac
    13381 ttaaactact atcaaaacct acattattct tcataatatc ccctcaaagc cttcattatg
    13441 gatttagctt tatcaagtgt ttcttgatat tcttcatctg ggtcagaatc ccaagtcatt
    13501 cctcctccaa cttgaaagta gacttttttg tcatttttga ttattgttct aattgctata
    13561 ttaaaatcca tatcaccatt aaaacctata taacctattg aaccagtata tacatttctt
    13621 tgcgttggct ctaattcgtc tataatttcc attgctctaa tcttaggtgc tcctgttata
    13681 gacccccctg gaaaagttgc ttttattaca tcagtagcat ctttatcatc ttttaactca
    13741 cctacaactg tggaaactaa gtgatttaca tttgcatatg gttcaattac aaatagctct
    13801 ggaactttaa cacttccagt tttagatatt ctcccaatat catttctttc taaatctact
    13861 atcataagta gttcagctct atctttttca ctatttctaa gttcttgttg aagtcttaaa
    13921 tcttcttctt tatctttccc tcttggacga gtacctttta ttggtctagt ttctattctc
    13981 ttattaacac atcttataaa tctttctgga gaatttgata atatatgtgc atcttcaaag
    14041 tttaagaatg ccccaaatgg tgctgggcta aatcttctta agtccctata caattcaaag
    14101 cttgtaagtt ctgtttctcc actgaacctt tgtgttaaat tagcttgata tatatctcct
    14161 tgtcttatgt actctctaac actttgaact gcatttttaa attcttcctt tgtaaaatta
    14221 gatttaagtc ttataggtgt tacttctttt tcttcataac atatgctatc tatacctttt
    14281 ttctcttctt ttaatattct ttgttctata tcatctatta tcttttcttc taattcgata
    14341 tctatgtttg gagtagctat ataagttttt tgcacaagat ggtctataac tattacatga
    14401 ttataaaatc caaaatacat atcaggcatc tcaatatcat ctacagcagt tctaggtaaa
    14461 ttttctatgt aattacctaa atcataagat aaatatccca cagcacctcc tacaaatgga
    14521 agattagtat catttacaac cctatactta tgtagttctt cttttaaaac ttctaaagga
    14581 ttttcatcag tatctttata ctttaatacc ttaaatggtt ggctacttat aaaagaatat
    14641 cttcccaatt tttctttatc catagcacta tctaatataa aactgtcatg ttcatttcta
    14701 aaaatagtaa atatctcaaa agaatttaat ttagtattta tctctcttat catattacat
    14761 atgtaccctt tctctagctt ctgttataaa atttttaagc atttcatgac catactctga
    14821 caaaatagct tctggatgaa actgtactcc ttctattaaa tatttcttat gcctaattcc
    14881 cattatttcc cctttatcag taattgctgt aatttctaat tcttttggaa ctgtattgga
    14941 gtcaactatt agtgaatgat acctagtgac atttaaagga tttttaagtc ccctaaaaac
    15001 tcctttatta gtatggttga tactatatac ttttccatgc acaggctgtt gtgcttttat
    15061 aatatctcct ccaaaaacat gtccaatagt ttggtgacct aaacaaattc ctagtatagg
    15121 ttttttcccc ttaaaatgct caacaatatc tatgcaaata ccagcttctt ttggagaaca
    15181 cggtcctgga gataacacaa ttacctctgg atttagttct tcaatgtcac ttattttgat
    15241 ttcattattt ctttttacta caactgcttc ccctaattct tctatatatt ggactaggtt
    15301 gtatacaaaa gaatcataat tgtctatcat aagaatcata ctgtaaatct cccccctaaa
    15361 aattatttta tcaactcaga aactgttaca aatttaaaac ctttttcaat catttttgga
    15421 ataaacatat ctaaagcttc aactgtatca ttagttgcat aatcatgcaa aagtattata
    15481 tctccatttt gtactttatt aattatttta tctgcaatgc tattagctcc tggatttttc
    15541 caatctttca catctacacc tgtccataaa acaactttat agtcatgctt ttttgcaatt
    15601 tctattaagt ctttttctct ataacttccg aatggtggtc taaataatgt aggttttttc
    15661 ccagttactt ccttcaaaat gtcttcacac ttaacaatct cctcttctat ctgactagaa
    15721 cttaaattac ttatatcagg atgattgaaa gtatgatttc ctatctcgtg accttcttta
    15781 cttgctctta ctaatggttc tttataccat tttgcatgtt ttccagctat aaaaaaagta
    15841 gcttttacat tgtatttctt taatacatct aaaacttgac tagtttcttt aggatgagga
    15901 ccatcatcaa aggtaattgc tattagcttt ttttcccttg aaccatgttt aatcaaaaca
    15961 tttccattag tatccatcat caaagcagaa actttactaa aattagatat taacactatt
    16021 ccacaaaaca aggctactgc tattaagcac ttatatactg tctttttttt ccatgatgtc
    16081 attttttaac ttcctttcaa atattttagt tatttataaa aattttgagt gtaatatgtt
    16141 ttatagcttc ctccaaatat tactccaact ccaatattat tatagttccc caaaatattt
    16201 tttctatgtc cttcagagtt catccaagct tcatgcgcat atattgcatt aatctgtcct
    16261 gctgctatgt tttctccagc tgaagtatat actatacctt ctcttttcat tctgtcaaat
    16321 ggagtttcat tcttcttatt tacatggtca aaaaaattat tatctctcat atcttcacta
    16381 tgttttcttg aactcaaagt tgcctgttca ctgtatctca atgagtttaa ccctctttga
    16441 tttctaacac tatttacaag gtctataact tcaagttcaa aacatttttt aagttcttcg
    16501 ctatcatctg gatataaatt tttaaactca tcttcagtgc ttttatcaat tattaaatat
    16561 gaacaaactc tattttcttc atgaatatcg tagaaaattg taatatactt accttcctta
    16621 cttattatat catattgatt ttctgagttt attatatatc tggtatttcc ttttctctta
    16681 tattttaatg gtgtaatctt agttctcact gtttgtctat cttgatttag ttttatatct
    16741 tgattttcac atgaattaat agaattacta taaagtgcta ctacttcatc attttcaagt
    16801 ccaaccattg caaattcttt tccatattga ttatatacat accaatcaaa accatattca
    16861 ctaatatcaa ttctaccagg tttgccgata cttgataaaa catagcttaa attatctcct
    16921 aagcttattt tttcaaaatt ctttatatct gactctccta agttctcccc ttcagtatta
    16981 tttgattgta aattttgact tttttctttt ttatctttct tagaattatc atttatattt
    17041 attttgtcta aaaattcatt ccatatttca ctttgaaaaa acttttcttt taaatcatat
    17101 ccttctttta ataattcagg actataaaat aaaacaataa gtactactaa cactaaagca
    17161 attaatctct tcaatatatc tctccttctt aaaatcaatc aaaatttttt aatattgcat
    17221 ttattaagtt aatattgata gtgtatacta atcaatatca ttcataccta tttacagtaa
    17281 gtattagaaa aaagctttat ctactaatta gataaagcta cagtctcaag gaaaatattt
    17341 catctaatgt tctatcaaat atagatggct tatcaaattt ccaagtccct tcataaacca
    17401 attcattatt tagataaaaa gttccttgcc catgtttttt accatgcata aattcaccta
    17461 tatacttttt acctttttcc catatataag ttcccttacc atgaatcaaa ttattcttcc
    17521 attctccttc ataaagtact tcccctttac tattatagca aataccgtac ccttccatct
    17581 ttccaccttt aaattcacct gcatatatac atccatcact ccacttataa ataccttttc
    17641 catctttttt tccgtttttc caagttccaa tatattcatc tttactacta tataacatat
    17701 ttccaatacc gctcatcata tcttcctcaa aataacctat atattcattt ccatttgaaa
    17761 atataaagtt tccttttcca ttttttttat cttctttaaa ttctcctatg tactccataa
    17821 ctgttccttc atctacaaaa aaagtatatg agcctttccc ctccatcttt ccctctaaga
    17881 atttccctac atatacatcc ccatttgaaa atttatagat tttatttttt ggtttttttg
    17941 tatcgacatc atctatatcg cgtattgttt ttccttccaa ttctaatact tcattttccg
    18001 gagcaaaatc gccccttttt aaaacacgtt tcttcatgtt ctttatactt ctaatctctt
    18061 tgtttacttc atttatatat ttcaaataat tatcatacaa attaacatat tctttagata
    18121 aattcataat gccattcctc caacgttatt cctaggtatt ttactaaatc tattaaattt
    18181 attatatcct aatagtggtc atgttttcta catattaagt aattttttca attttaattt
    18241 taaaatgata tcttttaaag tgatacacaa atcattaata aaacctttat tttcgtatac
    18301 taaatttaaa taagctgaga gatataatat acaaatttac attataaact cccagcttgt
    18361 aaaattttct ataaatcttt tgctttaaac cctcttccct ttacttctgt aacatcattt
    18421 acagatataa aggccttttt atcatacttc aaagctatat cttttatctt tggtatctca
    18481 tttgatgaaa ctattgtata tataatcttc tttttttgat gagtgtatgc accttctgca
    18541 tctaaaaaag ttacacctct aactaaactc ttcattatat cttctgctat taaatcgact
    18601 tcatttgaca ttaccataac agatttttgg taattcattg catctttaat aaaactcatt
    18661 gaatacgcat ttagaaatag acctataaga gtgtataatg ctaaatccaa cccaaataaa
    18721 aaacttcctg taagtactat caagccattt attgctaaag cagtgtcttt catttctata
    18781 ttcttcttaa tttttagtat tgcagctatt atatctgtac caccttgaga agacctagtt
    18841 ctaaatacaa ctccgactcc taaaccagat aatataccac catatacact ttgaagcaat
    18901 atatcatgta taggtataat attacttatt tcctgagttg ctcctaataa taatgaaaat
    18961 acaatcatat ttaccaaact tgaaatacaa aactcttttt caaggtatat gaaccctaag
    19021 ataaatattg gaatattgat taaaaatgtt aataaaccta catttatatt tgtcaaatag
    19081 tttatagatg ttgctatacc tgtagcacct ccactaagca gatgtgctgg tcttaagaac
    19141 ccatttattc ctatagatgt catcaacatc ccaaatagca ttgttgaata tcttaaaact
    19201 ggatgttttt gtagggtatt cttaacttca ctcatttctt tctcccttca atctttctaa
    19261 atctttagaa ttttaaaagc ttactcttac atatattatt cctgatgtaa tagataatat
    19321 ttcaaataaa ttttttttat aatttcttga actgtcatct attacatcat ctattatatt
    19381 atctttttca tatatgtcta gtgcaatctt ttatattcta atctttaaaa acgttcaaat
    19441 catctcattt atgccttaaa tgaaactttt tctagcataa tatgatactg cttattattt
    19501 aacttgcaat atagtaaatt tatagtattt ttttctaaat atctatttta gcagtaattt
    19561 attttatttt agcaaattca agtttagtaa ttgtctcttt ataattaatt cctaaatgaa
    19621 atttttcttt ttataatttc agtacatgat ttttttattt cattatttgc atttttttcg
    19681 ttcagttttt attttaaatt ttttttattt tattttgttt taagtattca aggtactcat
    19741 cttaatttta ttttttatat aaaattcatt ataattggaa ttagtattac tgtaattgta
    19801 ccagagactc caatagaaac accactcata gctccttcta tttctccaag ttcaaaagcc
    19861 tttgtagttc ctaaagcatg tgcagatgtt ccaagagcaa ttcctttagc aactggatga
    19921 tttatattcc ccagtttgaa tactgtagaa gcaaccatac caccaaaaat acctgtaaaa
    19981 attatggcta caactgttat tgattctaca ccatttaaag aatttgctaa agatattccc
    20041 attggtgttg ttatagattt tggaattagt gaatttatta tacttacatc tgaattagct
    20101 attttgccaa ttatcaatac agatacaaaa gaaatcacta taccacaacc tattcctaca
    20161 ataatctcaa acatatgctt tttaaataat tcaaattgtt tataaagtgg aactgcaagt
    20221 actactgtga caggacctaa aaagaaattt agactatcag ctcctatctt gtaattctca
    20281 taagggattt tagatatgct taaaaacaat attattccta cgattgcaat taataaaggg
    20341 ttacatatag gattacttgt ttttcgttga atgtatcttc ctaaattgaa aaaaactatt
    20401 gttactatta taccaaatac tggtgtctgt aatacattat acattttttt gtccttcttc
    20461 cttctttttt atcattttct ccacaataat tccagtagta tacattacta ttagtgtact
    20521 taaacatatg actaataaaa gcactaatac attttccctt attaggtcta atgactttat
    20581 aagtgataca cctgctggaa taaaaaatat agccatatta tcgaggaaaa atccacttac
    20641 attttctata ctactcaagt ctaaaacttt aaattgtagt agtagaaata ataatagcat
    20701 accaactata ctcccaggaa taagtataat tccttgtata aaggaactta tatactctcc
    20761 tattacccag atagataaaa ttattaccaa ttgattgaaa actttcattt tccacctcca
    20821 aaaattaaag agattgcttc aaatcatata tttgtagttt tatcaacaag caaatatctt
    20881 tctttaaaat agaaagttat aatttgacac aatctctttt taaatttatt tttattttaa
    20941 tttaataaaa tacatacttg gttgtttcaa aaaacttaac tatataaacc tatttttcac
    21001 ttaaaatttc ttttattttt gaattgttat acatactgtc tggaacttca cttctcaaac
    21061 tataaaaagt ctgttgtgca ttttttaaat cgccattatc ataataaagc atacccaact
    21121 cgtaatatga atcatcataa tactggtcat ttttagtata agtatttata tattttttgt
    21181 aatattttat agcttcatcc ttatttccta ataatttatt tgttattgct aattgatata
    21241 ttgactcaga aatatatttt ttagttttac cagaagaaac tacactttta aaattatcta
    21301 tggctttttc atattctttg tttttcttta aatttagagc ttgataatat aaatcttcct
    21361 catcagctac agttatctct ctttctggtt ctttattttt aacctcattt aattccttat
    21421 tcgttttttc taatttttta ctcgtttctc ctaacttttt atcagtttca cttaactttt
    21481 cctctttact acttatttgg ttattcaagt tttctattct ctttttgctt tgaatcaaat
    21541 atatccccat acctattaaa acacagccaa ctataatata tggcaaaata cttttctttc
    21601 tttttacttt ttttatttct tcctttttac ttgtatcttc tttttcttct tcattttttt
    21661 caaatacagg attatcttta tctagctctt taagtttgtc taaatatcct tctctctttt
    21721 ttacattacc tagtttatca tataacaaag ataaaattac atatggctct attagttcag
    21781 gttcttcttc aattatgttc tctaaaatat gtattgactc ataatttaat tcttcattta
    21841 taaatcttat agaatgattg tatcttccta aaaaagtttt aaaatcttca gatgaaatta
    21901 gatttacata ttttctagat atatgattat tttcataaca taaagcagta taaaaactct
    21961 caaatgattt agcaaaatca cattttaata attttaaaag acctctaaga tttagtatat
    22021 caatgtcttt aggatttatc tccagagctt tattaaccat attaatggca ctagttatat
    22081 aatttttata tgctaaatcc aatgcattgt tatatctttt atatgattct aattttaact
    22141 ccttagacgt tgtatctgac tttgaattta aagctccttg caagtttaat agagtttctt
    22201 ctttataatt tgtagtcaca taactctgtt ttaatgcttt taatgtttca tcaccgtctt
    22261 cttcttctat attaaaaaaa gtcaaatcac ttttattatc ttctatcaaa gatatatcta
    22321 aaatttttct aggcatgctt tctatgtctt gttttatatc tttaactaaa ataatactca
    22381 cctccactct aaaatatata tttaagataa catatttaat atgtcaagtc aaaactatac
    22441 atatttttcc accattctca taataaaaaa ggtaatcctc ttcttttcat aaaaaggtta
    22501 cctctttttc aatactatta acttttaatg ttgaattttt ttacttttct atagagtgta
    22561 gctataccta tatttaatgc ctcagctact tttttcatac cttctgtatt acttccatat
    22621 ttctttatgg cattttctat agcttccctc tcaaggtcat ctatagtctt aacttcttgt
    22681 ttataaagtt catctagttt aaaagatatt aaatcagaca tactccttac aaaagcagtt
    22741 aactccttat cttttgaaag tatcttttct ttttgttctt cattaaacgc agctataccc
    22801 aagattccaa gtatatgctc tcccatttta acgggagtac atatatcagc taactcttta
    22861 caattttctc tatttgcaca tttattacag ttctcttcta tttctttatt tataacatag
    22921 gatttcccat cttttaaaac aagagagtac actgacttct ctgatattgt ctctcctatt
    22981 ttacctctga atattcctgt acctgctatc ctagttaaat cttttgtaac taaagtaaca
    23041 tctacatcca agacattaga aatattctcg caaatacttt gaacgtaatt cgttatatta
    23101 tttaaattca aagtaatccc tcctatttag atttatattt tttattactc tacataaatc
    23161 ctattcttga gaactttaag cttgcggttg tggttgtgtc tcattaggct tattatcagt
    23221 tggtggtgtt tctggttttt gttgattatt tgggttttga ttgtttgtat tttgattatt
    23281 cttattttcg tctgtatttg gtttttcctc tggttttggt tccatagtcc caactaatat
    23341 aataccttcc ttcttaggat aatatgaatt agctattttt tcagttttaa tttttttacc
    23401 atttgcatca ttaaatgttc tatatgttga tacagaatat gcatttcttc cacttttgtc
    23461 tactttttct ttacctttag gcattgtagg gtcattaact cttttcattg tagtttgtga
    23521 aacgccatct aattttgttg atattgttat attttgttta tcttctgcac ttccatatat
    23581 attgcaaaca atttgatttc ctgaaacgta attttttatg taaactggat gctttagatt
    23641 atttttaaat aagaaatcta ttccactatc tgcaacagtt gcatctcttc cttttggaac
    23701 ataacttgat ggtatagtat gattttttac attaacaatt tctaaacctg cgtatagcac
    23761 agaattgtat aacgtagatg aaacttgaca aactcctcca ccataatctt cttccatctc
    23821 accttccatt attacaggtg cacttttata tccattttcc ttacttcttt tccctgtatg
    23881 tttattaaat gagaaagttt ctcctggcat aagtaataca ttacttatat tatctgttgc
    23941 taattttatg tttatacttc tatttatttg agaagtttta aatgttgttg aatacgtacc
    24001 taaatttgta tctatattag caagttgctc tttttttact tttgctggag tagcttttac
    24061 tactaactca tttttatagt tacctttttt agtttgatta tcaaaattct ccatactctt
    24121 agaaatatcc attttaagac catcagaatc tggtataacc ttgacttttt caccacttat
    24181 atcaagtgta gcatctttta catctacatc tatttcttta gctatctttt ccatttctgc
    24241 ttttaattta tttttatcat aatttatcgc aactactaag ttgctttttt tgccaaaatt
    24301 tgctgaaata gtttttgata gattgccaaa gaaagcactc tttctattta aattatatgc
    24361 gttttctact gttttatcca ggtcagtaga taaatttaaa tctttgctct ttactttcca
    24421 gcttttatca ttgtaattaa aatcaacatt ctctaatttg tattttttag ctaattcctg
    24481 ttttgcttgt gatttagtta attttccaat atttactcca ttaacatatg tgttttttgc
    24541 tattttctcc cctttaaatt gcatgcacat tatgcctata aaaattatta ctactaatgc
    24601 tacagctcca atagctataa ctagcttttt gttcacatta ctcatcccat acttctcctt
    24661 acaataaaat tatttatata tccctattat tatattacta cataatgcgc cttaaaaccc
    24721 caaaaacttt ttaattatat attaatttaa tattattaat taatattcat ttagtccatt
    24781 ttctgatatg gcatatattt tttcattgta aatattaata gcataaactt ctttgcctct
    24841 aaataagcct ttatttacta cagcataata ataaatattt ttatattttc caaacaattc
    24901 ttctaaactc tttttacttt ttacatatct atctgctttc tctatatcta atatctcaat
    24961 ctttttatac ttattttctt tttcatcaag agtattagtt aacacaaact tagctttatc
    25021 ttttgtaata ccaaattgat aacttccatc ttctaatatt ctaaagtatc catcaccata
    25081 atctactttg taatcttttt taccatcttt atatgaatat aaatctttag tttttgcact
    25141 taccaaaact tcatttatta atttatcata ctcatatact tcagcataat agtatttatt
    25201 attttttatc atttgtggaa gtgaatttat agatagattt gccttgtctt tgccactttt
    25261 tttagctatt acatttataa tgtcatcaga tgttaaagta tacaatgata aattcttttt
    25321 atattttttt tctaaactct gtaccttttc atcatctgaa taatcgtctt ttaaatactc
    25381 aatatattgt aatgctcttt cataatctcc agaagtattt gcctcttcag ctctacttat
    25441 atagtaatca tacatttctt caatacattg ttctttccca ttttgagcca tactgtaata
    25501 cttttcatct tcttttaata ctttattata cttttcaata gcttcataat acttatatgc
    25561 tttttggtct tttactgctg agtcataaac atctctagac tctttgattg tttctatatt
    25621 ctgtttgtat acatctaggt tactacccat accatttaat attgaaacag tacttatgta
    25681 gttaatggca atatcataag tagtattttc ttctttatac atttctctaa ctctatcagc
    25741 ttgagctaat aagtcatcca catctatact tattttgttt aattctttca cggtatttat
    25801 taatccaata aaactttctt gacttatgtc tccatctaaa tatttatcac cactatttaa
    25861 tagcaattta tttatttttt tagaaacaga cttattaaac ctttccattt ttgaatcaga
    25921 aaactttttt tctatacttg tgtagtaatc aacagctata gaatactgtc ttttctctat
    25981 aatctttaac aactcattag cttcatttcc tacactaaac tcgttactac ttaagaaaaa
    26041 tactattatc aaagcaatta cacctaatgt tgataatgaa atcgcaacta tagatttcct
    26101 tttttcatca ataaaatcta cagccttctc aaataaatct ctaaaattca ttatagttcc
    26161 ctctccattt aataatcttt atattttatg gtaaactaaa tttcttattg gttattatat
    26221 cataattctt gaaaaaaaat aaaaacacaa gttaaatcgt gtttttattt tacctatcaa
    26281 tcattatttt atacataaat atttccttta cataaattat gatatctttc tttttatatc
    26341 cctagaatga ctcttttgtt ttttattatt actgacagtt ttcgttcttg tcttgtgtat
    26401 agctttgcta tcaaatattt ttctagcatt gcataaaaca attccaattg ttaaacaaac
    26461 tgtaagcaca cttcccatca aaaagtaata atccaatcct atatgtactc ccataactga
    26521 atttattaac actaatgcaa aagtcattgc aaaacatatc atcatggaga taactaaaat
    26581 tcttagaatt tttttcaatc cactcacctc gtataatcac taaaattgac actacccctt
    26641 atccattact ataccaaatt agtaaaaaaa tagcaattac tattgtcatt tatattagga
    26701 aaatattaaa tctttttaat aaatatcttt ttttgacata aggattactt aacttttaat
    26761 aaaatagtaa cactaatatg tattaattaa tagaataaaa taaatgataa tttattgagg
    26821 ggtgatttac atgccaaatt tgccaagttt agggtcaaag gctcctgatt ttaaagccaa
    26881 tacaacaaat ggtcctatta gactctctga ctataagggt aattggattg ttttattttc
    26941 acatcctggt gattttacac cagtttgtac tacagaattt ttatgttttg ctaaatatta
    27001 tgacgaattt aaaaaaagaa atacagaact aattggtcta agtgttgata gtaacagttc
    27061 acatttagct tggatgtata atatttcttt acttacaggt gtagaaattc catttcctat
    27121 tatagaagat agagatatga gaattgccaa gttatacggc atgatatcaa aaccaatgag
    27181 tgatacatca actgttcgct ctgtatttat tatagataat aatcaaattc taagaacgat
    27241 tctttattat ccactaacta caggaagaaa tattccagaa atacttagaa tagtagatgc
    27301 acttcagact agtgatagag ataatatagt tactcctgca aactggtttc ctggaatgcc
    27361 agtgatttta ccttatccta aaaactataa ggaattaaaa aatagagtta acagttgtaa
    27421 taagaaatat tcatgtatgg actggtactt atgttttgta ccagataatt ataatgatga
    27481 agaagtgagc aagaaaattg ataatacttg tagctggaaa aaagaacata ctaaaaacat
    27541 tgaaaatgaa tgtaactgtg aacatgaaca tcatgactac ctgaacaaag ctcttgactg
    27601 taaacaagaa cacaagactg atattaaaga tgattgcaat catgagaaaa aacatactaa
    27661 aaatactaac aaagttcaca actccaaaca agataagttt aaagataagt cttgtgatga
    27721 aatgaatttt aactatgata aagatgaatc ttgtgacaaa ataaattcta gctataacaa
    27781 agaagatagt agttatgaag atttctataa acataattat aaaaactacg attatactag
    27841 cgaaaaaaat actaaaaaaa tagctatgaa aactttaaaa gattctaaaa aattagttag
    27901 accacaaata acagacccat acaatccaat agttgaaaat gcaaactgtc cagatataaa
    27961 tccaattgta gcagaatatg ttcttggaaa tccaactaat gtagatgctc aactattaga
    28021 tgcagttata tttgcttttg ctgagataga ccagtctgga aatttgttta ttccttatcc
    28081 tagattttta aatcaattac ttgctcttaa aggtgaaaaa cctagcttaa aagtaattgt
    28141 agctattgga ggttggggag ctgaaggatt ctctgatgca gcattaacac ctacatctag
    28201 atataatttt gcaagacagg tcaatcagat gataaatgaa tatgctttag atggaataga
    28261 tatagactgg gaatatcctg gaagtagtgc atctggaata acatcaagac ctcaagatag
    28321 agaaaacttt acactcttac taactgccat aagagatgtt ataggagatg ataaatggct
    28381 tagtgtagct ggaacaggag atagaggata tataaattca agtgctgaaa tagataaaat
    28441 agctcctata atagattatt ttaatcttat gagttatgat tttacagcag gtgaaacagg
    28501 cccaaatggt agaaaacatc aagcaaatct ttttgattca gacttatctt tgccaggata
    28561 tagtgttgat gcaatggtaa gaaatcttga gaatgctgga atgccttctg aaaaaatcct
    28621 tcttggtata ccattttatg gaagattagg tgctactata acaagaactt atgatgagct
    28681 tagaagggat tatataaata aaaatggata tgaatataga tttgataata ctgctcaagt
    28741 tccatattta gttaaggatg gagattttgc aatgtcatat gatgatgctt tatcaatatt
    28801 cttaaaaact caatatgttc ttagaaattg tctaggtggt gtattctcat ggacatcaac
    28861 ttatgaccaa gcaaatatac tggctagaac catgtctatt ggtataaatg accctgaagt
    28921 attaaaagaa gaacttgaag gtatttatgg gcaattctaa tagaatgtag gaaaatttta
    28981 tagttatata aaaagcagat tttattatat taaataagtt gtatctaatt aatactatta
    29041 ttaattgttt ataaattaga tacttatttt ttcataatac aatctgcttt tttaatcaaa
    29101 atttatttaa ttataagttg tttaattaca agttgtttaa ttacaagttg tttaattata
    29161 aagtaggaat ttagatatac tcttatacca tgtaattatg tataaatatt tttttattat
    29221 acaccaatag cttttttagc taatttatct gcttcctcat tatacttatt tccagaatga
    29281 gctttaacct ttgtaaattt tacattaagc ttattcttaa tactgtcata gaaattttta
    29341 tatgctatag ttccttcttt atttgttttc caaactccag tacaccactt ttcaatccct
    29401 tcatagtcaa agtataaaat caagttttga acattattgt caatacagta ctgcattgca
    29461 attttagaag cctctatctc tccagctaca tttctcatac ttactaaagt tttctctttt
    29521 cctttttcac tataagtttt tataacttca ttatttttta aaatcacaac acccgaacca
    29581 tacattttta ctgaatgttc ataacttcca tctacatatg cttctataaa ctccttatct
    29641 tcttgaaaac tcaatttttc tgacccatca atatattctt tagcttcttg aaaagtagaa
    29701 aaactcttat attcagctcc tgagaatcca tttacttgtt ttttgcactc atcccatgta
    29761 ttatatacac ctatattttt accttttttc acagcataaa acttttgttt tgccaaatat
    29821 atcactccca ctcttaattt ttatcttctt ctctcataaa tatatctaaa tttataagat
    29881 agtttctatc aaaatatttt actttcatat ctttttttat aatatcaata tggtacactt
    29941 ctccttttac agctctttgt cttatatttt catttatatc tttttttata atatccttat
    30001 caacatcatt gttatccatt ttagcatcaa cattcaacgc ttttatagta tcaaatataa
    30061 cagattttag tattaaactt ctatatttat ttaaatgtcg ccttatataa gtataaaact
    30121 cattgctatt aacctttgaa attttacaat cgtgtaaatc tttctctaat ctctttaaca
    30181 cacttatatt agttcctaca tcaaaaatca caaaacatcc atagtcatat ttgcttctta
    30241 aaattctccc catagcttgc tttaccttta tggtcatttg aggataattt atagtatagt
    30301 agtctatacc atacttcttc attatagtaa aatacaaagg gtcttttgga tttagattcg
    30361 gaagcttgtc taaagttaca caaaccaaac catcccctgg aatgtctacg ccttcaaaac
    30421 atccttttga gccaagtact acacattttc tatttaaatc gttaaggtgt ctaatcccct
    30481 ttttatcagc ataaatttct aagttaaaac tatgtaagta ttttttcaat atttcataag
    30541 ttttttcttg tctatcttta ctattaaaaa gagctaatgt atgaccttct gtaatctttg
    30601 atatattaga tataatttta ctcatctcat ttggaaattc agaatttctg tactcacaaa
    30661 tatcagaaaa tccaactaca gagactctat ttttataatc aaaaattggt tctataatct
    30721 tttccacatt cttaactctg tctattccta atgtattttt aaaataagac atactttcag
    30781 ataaactcaa agttgctgat aaaaatattc ctttttctaa ttgagataaa atattttctt
    30841 caaataaatc tgctattttt aatggcacta ctcgaaattc aaagtcatta taattcttat
    30901 caatttcaac tatccttgca taatcatcct tttcatcata ctctaaaaag atttcaaata
    30961 taatctttat atcctctaaa tctcttgtct ttgcctttcc aaatttatat atatctgctt
    31021 ctttatctat actatcatca tcaatattcc tataaattat tattattatt gaaactaaat
    31081 tttttattat tttctcacaa ctcaacttta tcttttcact ataggctcta taacttattt
    31141 cagtatctat accatccttt tttagttttc ccacaatttc gtctatttgc aaattcagtt
    31201 cccatcttaa attataatta cttacattat tatattcatt acaattacca aactttaata
    31261 ttgaatctat ctcttcaata atgagattta tacttctagc aattttttgt ttatcttctc
    31321 ttccaatttt taatacatta taaaatttat caaaagcctt tatttttctc atatttctac
    31381 tatatttttt atatataaag ctactatttt gaataaactc gtaaggataa atttcttgta
    31441 ataaatacct taaagacttt gaatttataa tgcttgaaaa gaaatcatat cctttttcag
    31501 tcaaattatg tgcttcatct actattatat tttctaaagg tttttcatcc ttatatggcc
    31561 atttagctaa tagtgaatga tttactactg ttatatgttc ttcttttaat tcttctattc
    31621 tgtttttata taaacaatct tttttgcatt ttttaggtct gcacatattt gggtcacaac
    31681 ttacatttct taagtgagta cttatttcct tgaagttgtc aaacactaaa taatttattt
    31741 cttctatatc tccatacttg ccaccttcaa ctaatctctt caaaaatatt aaagataaaa
    31801 tttctccttt agtagggttt tgagactcat aatcatctat atatgcttcc agtctatcta
    31861 tacaaatata gttatttttt ccctttatat atccgtaact tacttttcca tttagaccca
    31921 aagaatctaa cacatttgga atatctttat ttattaattg tatctgcaat tcctttgtgt
    31981 cagtagaaat taataatctt tttttattaa ttctagcctc taatattgca ggcaataaat
    32041 atcctacact tttacctata ccagttggtg cctctataca tgcaattttt tcttcatctt
    32101 cgctatttct aaacaactct cttattgttt tagtaagctc atattgacca ggtctatatt
    32161 catatgtaaa tccttcttta ttctcccaaa tagttttgta ttttaataat tcttcataat
    32221 ttttttcttc ttcatttaaa atttttaagg tttctctttc cttcttcttt tcttcttttg
    32281 agtcaaatat attatattct ttttttatat ttatattatt tgataaatca taatttgcct
    32341 cttctaaaaa tttgctccat tcccatgttg gaagattaaa ttttttaaga tatgaattta
    32401 ttttaaaact caatggctcc aaagtagttt tttcatagtt attaaatttt actaaaaggc
    32461 aatttacaat atttattata tcaatagcat ctgataaagc tctattttcc actttagaat
    32521 tacaatttgt taaagtattt tttaagtatt ctaagctata atctttatga tatggttcta
    32581 aaataactgc taattcaatt aaatcaataa atttgttttt taactttggc atatgatact
    32641 caagaaactt ttttttcaga cttaaatcat gacatataat gaaactatct tctacaaaat
    32701 ttacaagtct atcttccacc agttctattt cattagctat ttctaaatca tttttatcta
    32761 aatttttaca tactgagaat acttctacag gtacctcaaa tttgtttttt attaatgatt
    32821 cataggtata tattttccag tctttaacct tgacagctcc aacttctaaa atttctgaat
    32881 ttaaacaatc taatccacta acttctatat ctaaaaaaac aacatcattc aatacactta
    32941 ttatattatc catttttata tcccttcaag ttaaaccagc ctaacttatt tacaatcatt
    33001 tctctttata ttttatcatt tatatacact ttatttaaga taattaatat caactttata
    33061 tcttacagta aacttctttc atttattatt tgtatatgtg aatattttta actataaata
    33121 ttcaaactat attataagaa atatacttta aattttaaat aagaggtgaa attgtgaaaa
    33181 aagatagcct aaaaaaatac attatgatag gagcatttgc tctaatactt tttggaattg
    33241 ctagtataaa ttttaaatca ttagataaaa caaaaactca aatttctagt ccaacactcg
    33301 acacacatga atatgattgg tattttaacc ctagggagga tggtaaacag ccatcaccta
    33361 taaaagaagc tgattttttt aaaaaatacg gagcttatta tgtaggaaat cccaatgaaa
    33421 aagttatata tctatcattt gatgctggat atgaaagtgg aaacactcca aaattattag
    33481 atacgcttaa aaaacacaat gctaaagctc aatttttcgt agttgaaagt tatataaaaa
    33541 gcaatccaga gttaataaag cgtatggaaa aagaaggaca tttagtttgt aatcattcaa
    33601 aaagccatcc atcaatggca ggtattacag attttgaaaa attcaaagaa gaaattacaa
    33661 gtgtagaaaa agcatacaaa gatgttactg gaaaagaaat gcctaagtat tttagacctc
    33721 caatgggtaa atttagcgaa caatctttaa aatatacaca agatttagga tataaatcaa
    33781 tattttggag ttttgcttat gtagattggt atgaaaagaa acaacctact catgaatttg
    33841 ccaaaaacaa aatttattca agaactcatc ctggtgcaat agttttactg catccaaatt
    33901 catcaacaaa tactgagata ttagatgagg ttcttacaca ttgggaaaaa gaaggttata
    33961 aacttaaaac acttgattat ttaaataata aaaaatagta tcataaattt aaaaaaactc
    34021 tatcatgaat tatagtaaaa atatagttat ttataaatac tttaattctc gatagagttt
    34081 tttatttttt atagctattt aaaaagaatt gtccattcat ttatctcttt gtatcctata
    34141 ttatgatata ttttacctgc tacatcatta ttataaaaaa gacatggaac ttttccttct
    34201 aacaacaatt ctttactcaa attgtaaacc atataacttg caagtccttt tcccctatat
    34261 cttttatctg tacttacaga gaccaccatt gccaaaaaac tagtttccat accagttgac
    34321 acagtgctaa tcatagtatc atcatttcta ataaaatatg ctcttacatt tccctctttt
    34381 aaatgaactt ttctttcttc tatgtaattt ttacttactt tatagtctga cctatttaaa
    34441 agttttccta tttcatcaat atctttttcc tgagcatttt ctattttata ctccttcatg
    34501 ttggaaaaat ctatttcttt tatttctttt aatacacaaa atttattatc tagtttttca
    34561 taaaaatctt tgtatttact tattagtcta tctattacac atttttttcc acttatgctt
    34621 tctacatcta aatcttttat atgattcttc atttctccta tattaaaatt atttttaaaa
    34681 ctataaatta taagtgttga cttatttttt aatattatag aggttatatt tccatcttct
    34741 acctgagacc atatatcttg atattgagag ctaaatccat aattctcaac gtatccaatt
    34801 atgaataaat ttaaactttc ttcatgtgat acatattgcc aaaattcttt ttctaaagta
    34861 ttatctattt ttacaatcaa gcagatatcc tccttctatg ttaataatga tataccttat
    34921 aatttatagt atcatttaaa atcattatat tctaccaaat attctaaatt tttaaatatc
    34981 tatgcaatat gttcgtattt atttttatat tctgttactg cttcactcca agcttcttct
    35041 actgaaatac ctctcttatt tgcaattttt tgtgctaatt ctattatttt catagctttt
    35101 cttgatatat tcatatatat tcctcctcta aattatcata agttatttta aagttaaatt
    35161 tcta
//
LOCUS       DAEOSP010000020        35106 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.020, whole genome shotgun sequence.
ACCESSION   DAEOSP010000020 DAEOSP010000000
VERSION     DAEOSP010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35106
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.020"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(125..1132)
                     /locus_tag="KRH54_000766"
     CDS             complement(125..1132)
                     /locus_tag="KRH54_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBG8578438.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            1360..2244
                     /locus_tag="KRH54_000767"
     CDS             1360..2244
                     /locus_tag="KRH54_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG8578439.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            2446..3996
                     /locus_tag="KRH54_000768"
     CDS             2446..3996
                     /locus_tag="KRH54_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG8578440.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            4025..4672
                     /locus_tag="KRH54_000769"
     CDS             4025..4672
                     /locus_tag="KRH54_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBG8578441.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(4814..5074)
                     /locus_tag="KRH54_000770"
     CDS             complement(4814..5074)
                     /locus_tag="KRH54_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBG8578442.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            5291..6574
                     /gene="hflX"
                     /locus_tag="KRH54_000771"
     CDS             5291..6574
                     /gene="hflX"
                     /locus_tag="KRH54_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG8578443.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVRLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            6582..7259
                     /locus_tag="KRH54_000772"
     CDS             6582..7259
                     /locus_tag="KRH54_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBG8578444.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            7345..7788
                     /locus_tag="KRH54_000773"
     CDS             7345..7788
                     /locus_tag="KRH54_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG8578445.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            8093..8737
                     /locus_tag="KRH54_000774"
     CDS             8093..8737
                     /locus_tag="KRH54_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBG8578446.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(8823..9200)
                     /locus_tag="KRH54_000775"
     CDS             complement(8823..9200)
                     /locus_tag="KRH54_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG8578447.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(9200..9586)
                     /locus_tag="KRH54_000776"
     CDS             complement(9200..9586)
                     /locus_tag="KRH54_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBG8578448.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            9765..10520
                     /gene="nadE"
                     /locus_tag="KRH54_000777"
     CDS             9765..10520
                     /gene="nadE"
                     /locus_tag="KRH54_000777"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBG8578449.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            10603..11322
                     /locus_tag="KRH54_000778"
     CDS             10603..11322
                     /locus_tag="KRH54_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG8578450.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(11489..12493)
                     /locus_tag="KRH54_000779"
     CDS             complement(11489..12493)
                     /locus_tag="KRH54_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG8578451.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            12759..13655
                     /locus_tag="KRH54_000780"
     CDS             12759..13655
                     /locus_tag="KRH54_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HBG8578452.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     CLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            13792..15054
                     /locus_tag="KRH54_000781"
     CDS             13792..15054
                     /locus_tag="KRH54_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578453.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            15130..16323
                     /locus_tag="KRH54_000782"
     CDS             15130..16323
                     /locus_tag="KRH54_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG8578454.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDKHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            16952..17746
                     /locus_tag="KRH54_000783"
     CDS             16952..17746
                     /locus_tag="KRH54_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG8578455.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDVAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            17841..19181
                     /locus_tag="KRH54_000784"
     CDS             17841..19181
                     /locus_tag="KRH54_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG8578456.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            19318..20550
                     /locus_tag="KRH54_000785"
     CDS             19318..20550
                     /locus_tag="KRH54_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBG8578457.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKDGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     TLIESGIVTMNKEACEIC"
     gene            20590..21732
                     /locus_tag="KRH54_000786"
     CDS             20590..21732
                     /locus_tag="KRH54_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBG8578458.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            21743..22537
                     /locus_tag="KRH54_000787"
     CDS             21743..22537
                     /locus_tag="KRH54_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG8578459.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            22548..23570
                     /locus_tag="KRH54_000788"
     CDS             22548..23570
                     /locus_tag="KRH54_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG8578460.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            23669..25045
                     /locus_tag="KRH54_000789"
     CDS             23669..25045
                     /locus_tag="KRH54_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG8578461.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(25377..25721)
                     /locus_tag="KRH54_000790"
     CDS             complement(25377..25721)
                     /locus_tag="KRH54_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578462.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            26209..27219
                     /locus_tag="KRH54_000791"
     CDS             26209..27219
                     /locus_tag="KRH54_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG8578463.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            27355..27843
                     /locus_tag="KRH54_000792"
     CDS             27355..27843
                     /locus_tag="KRH54_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG8578464.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            28019..29947
                     /locus_tag="KRH54_000793"
     CDS             28019..29947
                     /locus_tag="KRH54_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG8578465.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            30124..30534
                     /locus_tag="KRH54_000794"
     CDS             30124..30534
                     /locus_tag="KRH54_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBG8578466.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            30556..30858
                     /locus_tag="KRH54_000795"
     CDS             30556..30858
                     /locus_tag="KRH54_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBG8578467.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(31075..31767)
                     /locus_tag="KRH54_000796"
     CDS             complement(31075..31767)
                     /locus_tag="KRH54_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG8578468.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(31793..32980)
                     /locus_tag="KRH54_000797"
     CDS             complement(31793..32980)
                     /locus_tag="KRH54_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8578469.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            33316..>35106
                     /locus_tag="KRH54_000798"
     CDS             33316..>35106
                     /locus_tag="KRH54_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG8578470.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNIGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNT"
BASE COUNT        13282 a         4029 c         6446 g        11349 t
ORIGIN      
        1 atttatattt tatactatgt aaaatatcta atatatttgg tctgtaggtt aaataaaagt
       61 acctttagac caaatatatt attaataact taataacatc ttaaaactta tttatttata
      121 ttatttacaa catccattta ataaatacac cataacctac agctgggtcg ttttcaattg
      181 catgtgacac tgctccgatt attttattat cttgaactat tggagtacca ctcatacctt
      241 gtacaatacc accagtttga gctaaaagct ttttatctgt aattttaatt ttaaaacttt
      301 tactttctgg atgtttttgg ttttctatat ctataatttc aatattatat tttttacact
      361 tattaaagtt atcttgtaaa attatttgag cttttcctaa tttaacttca tctaaagatg
      421 ctactttata tttttttaac ttggaaatat caaagttatt aatttgacct cttattccaa
      481 aatcagtatt ttctgtaaag ctaccaataa tattttctcg ttttgcattt atacatccaa
      541 cactaccttt ataagatttt tctatattaa ggtgcttagt tgtagaaatt gaacctgttt
      601 taataggtat ttttcttgca ttacccacac ttattggatg acctacagct ccaaactctg
      661 atgtatctgg attgatatac gttaaagtag caaatccaga aagtaaattg ttaaaactaa
      721 ttttttctaa tatgtgtttg gtagttttca ttgttataag ttgtccacct ctatttaata
      781 aaatagatac ttgtttttct tctggtaaag catttaaggt attggcaaag tcactatatc
      841 catcaacttc agcattattt acctttacta tagcatcacc caatctaaat ggagatgctt
      901 ctccaggatt tctaactatg atatttttta attctgcgtc aatttgaagt acgtttccaa
      961 ttggtattaa atattcaggt tcattttttt gtaaattttg tgcataaatt aaattatttg
     1021 agaaaaaata taataaaaat aaaaatgtta aaacaattgt aaattttgac ttagctaagt
     1081 tggttttttt acttaaagta ttatttttaa aatgaaaatt tttaagttgc ataaaacacc
     1141 tctttcttat taaattattt taataataga attgaagttt tcttgctgct tctatattat
     1201 gttgtgcaag aatcatgttt tgatgcttgg aaaactagct caataacacc ttagttttca
     1261 taaaaataaa taaaataaat agtgtatatg ataaaataaa tacatattca tataaataaa
     1321 aataaacaat aactgtctag cataagaaag gagcaaaaaa tgaaaaaata tagattgtta
     1381 gttacttgta taattgccat gggaatcata attataggaa catcaaatcc agttagcaat
     1441 ttttacaaga ctacaaattt gattagttca ggaaatatca caaatgaaaa agcagataaa
     1501 gatgaaaatt caaacaaaaa taataaagat aaaaatgaag acaaatctaa ttctaaaagt
     1561 aataaagata agtcatcaga ggacaaatcg tcagataata aaaatgaaat tcaaaaaaat
     1621 aaaaaacttt taatttgtat agacccaggg catcaaggaa aaggagatag caatttagaa
     1681 ccagtagctc caggttcttc ctctaaaaaa gcgagagtat cttcaggaac agagggtatt
     1741 gctacaaaaa aaccagaata tgttttaaac ttagaagcat ctcttgtttt aaagagtata
     1801 ttagaatcta aaggttataa tgtaataatg actagagaga cacatgatgt aaatataagt
     1861 aattcagaaa gagcaatact tgctaatgat aaaaaagcag atatggttgt aagaatacat
     1921 gcagatagtt taaataattc ttcaaaaact ggagcatcta tattgattcc tgaaaaagat
     1981 ggaaaatata cagctcctat atatgaagaa agtaataaat gtgctgaatt tatcaaacag
     2041 aatatggaac aatcaggtat acaaatcaat ggaatcgttc aaagaggcga tttgactgga
     2101 ttcaactggt caaaagtacc agctgtatta gtagaaatgg gatttatgag taactataat
     2161 gaagaccaaa tgatgtcaaa tccagactac caaagaaaaa tgatgcagtg tatagctgat
     2221 ggattggatg cgtattttaa ataagatatg aatagagaaa aagttaggtt ggtattattt
     2281 tcctaatttt tatctctatt ttttattgta cataacacaa agaagaagtt tattttaggt
     2341 taaattgtcg taaatataac taaaaatatt gggaatactt gatttaagtt taaaaagaat
     2401 atattatagt atagaatgtg taaaattttt ttgagaggtg ttgaaatgct agtagtagaa
     2461 aatgtgagtc atggatttgg tgcgagaaca atattagaaa atgtatcctt tagacttaga
     2521 aaaggtgaac atattgcact agttggtgct aatggagaag gaaaatcgag ttttttaaat
     2581 ataataacaa agaagcttat gccagatgct ggaaatataa aatggtcttc aagagctaca
     2641 gtaggttact tagaccaaca tacggtatta agcaagggaa aaactataag agaagtgctt
     2701 agagaggctt ttaagcatat gtttgactta gagcaagaaa tgattgctat gtatgacaaa
     2761 atgggagaag ctagtgatga tgaaatgagt aagcttcttg aagaaacagc agagatacaa
     2821 actatacttg aaaatagtgg tttttatatg atagatgcaa agatacaaga agttgcaaat
     2881 ggtcttggtc ttggagaaat tggtcttgat aaagatgtaa ctgacttaag tggagggcaa
     2941 agaacaaaag tacttcttac taaacttcta cttgaaaatc caactattct tatattggat
     3001 gagcctacaa actatttaga cgaagagcat ataacatggc ttactaagta ccttcaagag
     3061 tatgagaata gttttgtctt agtttctcat gatattgaat ttataaataa tacatgtaat
     3121 gtaatatatc atatggaaaa tggagagtta aatagatata aaggaaatta tgatgaattt
     3181 gtaagactta atgatataaa gaaacgtcaa gaagaacaag cttatgataa acaagttgaa
     3241 gaaagaaaaa gactagagga ttttgtagct agaaataaag ctagagtagc aactagagga
     3301 atggctaaca gtagacaaaa gcaacttgat aagatggaaa tattagaaag accaaaagaa
     3361 aaaataaaac ctacttttgc ttttaaagat gctagagcag ctagtaaaat aatttttgaa
     3421 acagaaaatt tagttttagg gtatgatgaa gcattgacaa aaccacttaa tttccattta
     3481 gaaagaggaa aaaagattgc cttaaaagga atgaatggta ttggaaaatc taccctatta
     3541 aaaactttac ttggaataat aaaaccattt gaaggaaatg ttaaattagg tgactatttg
     3601 gaagttggat attttgaaca agagagttca agagaaaata gtaatacacc tatggatgaa
     3661 gtttggagtg aatttccagg gcttacaaac tttgaagtta gacaggctct tgcaaaatgt
     3721 ggacttacaa atgaacatat aacgagtcaa atgagagtat taagtggtgg agaagctgct
     3781 aaggttagac tttgcaaagt aatgttaaaa aatataaatt tcttagtatt agatgagcct
     3841 acaaatcatt tagatgttga ggcaaaagat gaattgaaga aggctatcaa agagtttaag
     3901 ggaacagttc ttttagtatg tcatgagcca gaattttatt cagaaattgt tgatgatgta
     3961 tggaatatag aagattttac aactaaaatc gtgtagtgat ttaacttatt gctgggggga
     4021 tttcatgaaa aagtttctaa atgtactttt ttcaatttgt attagtattg ttataatagt
     4081 aggtgttatt aattttactg ttggatttaa acaactttat tattttgaca ttgattactt
     4141 aaatatatca gagttatctg gtttatctaa agatgatata aaattgaact acgattatct
     4201 aattgattat aacttaaata agaatgtgtc tgagttcaaa ttaccaacat taaaatcttc
     4261 tccacaaggg aagatacatt ttgaagaagt caggaatata tttcaaaata taaataaatt
     4321 agctaaatta ttattggtag tttctttagt aggaattata ttgagtgtaa aaaataaaaa
     4381 tataaaaatc ttaaaaacta catcaataac acttataatt atgccattat tattaacagt
     4441 tccaatttta ttaaattttg aaaagtcatt tattattttt cataaattat tatttagaaa
     4501 tgattattgg atatttaatc ccgatttaga cccagttata aatatattgc cagaggaatt
     4561 tttttttcac tctggaatga tgattttaat attaattttg ttagtaagta tagtactgtt
     4621 tgtaatgtat aaattatgta aatctttatc aataaaaata tttcaatgtt aaatagaggg
     4681 atatttttat ttccaaaata agataataaa aaaggattag attaaaagtt atacaataaa
     4741 aatagctgtg caataatatt tagactatga aagtagttga aaaatattta tgcacagcta
     4801 tttataatag aatttacttt ttactagtga ttttaactaa atcttgaaat ttactcataa
     4861 tgtaattgta attatctttt ttatgaggta atatacaact actgcaatct ttgatacctt
     4921 tatctgtata cttaaagttt ccaccacagt tttctcctaa agcatacaaa ggacaatagc
     4981 aaaatagaca gttaaactct tctggtttat tagttttatg acaagggaaa aattcgcaat
     5041 ctttgtgatt aaaaaactta taattttcgc ccatattttc ctcctaatta aataaaattt
     5101 aaatttttta tactatataa gattttggtg ataatataat tatactaaat aattttatga
     5161 atagtcgata atatagtatt attaatcata gtattaatgg tataattagt caacagaaaa
     5221 ataatgtaaa aataatgtaa gtgtattttg taattagaat atgttttata acaaaaaatg
     5281 aggtgattaa gtgaaagaaa ttcaagaaaa agcacttctt gtaggtctga atttgactac
     5341 aatagtgaaa aaaaatgatg atatagacac taatgaatct atggaagaat taaaagaact
     5401 aacaaaagct gctggggcag aagttgtagg tagcttaatt caaaataaac actctgtaga
     5461 tgcagcttat tatataggaa aaggtaaagt agaagaaata agagcctata gtgactcttt
     5521 agatgctact ttagtaattt ttaatgatga attatctggt gctcaaatca gaaatataga
     5581 gaatgttgtt ggaagaaaag taatagatag aactacttta attttagata tatttgctca
     5641 aagggcactt agcaaagaag gtaaattaca agttgaatta gcacaattaa agtatagatt
     5701 gccacgactt tatggaatgg gtggtgagat gagtagaaca ggtgcaggga ttggtactag
     5761 aggtcctgga gaacaaaaac tagaaataga taagagacat atacttaata aagcagcaga
     5821 tataagacgt gaacttaaag aagtaaaaaa gaatagagag acacagagag ttaagcgttt
     5881 aaagtcaaat ataccgattg tagcacttgt tgggtataca aatgcaggaa aatctaccct
     5941 gttgaacgaa ttaataaaga cacacaagga ttatgaacaa gaaaaagaag tttttgtaaa
     6001 agatatgctt tttgcaactt tagatgtaac tttgagaaag gctcttttgc cgaataaaaa
     6061 agaattttta gtagtagata ctgttggatt tgtaagtaaa cttccccatg atttagtaga
     6121 agcgtttaag gcaacattgg aagaagtaca gtatgcagat ttaatcttac atgtaattga
     6181 tgcaacaaat actagttatg aattgcaaaa aagtacgact gaaggtgtat taaaagaact
     6241 tggtgtaaat gataaaaaac atatacttgt atataataaa gtggacaagc tagaattgga
     6301 tatatatcct aaaagtcaag aagatattgt gtatatttct gcaaaacaag gcataaatat
     6361 ggataagtta ttaaatatga tagaaattgc tttaatggaa aatacctatt cagtaaggtt
     6421 gatgttacct tatgagagag gtgatatatt tagtagaata aaagataaat ataatgtgga
     6481 gaattttgag tatggggaaa gtggtataac gctagatgta gatttagatg aagaagattt
     6541 taatatctat agagagtaca tactagaaaa ataggttgaa tatggatata aactgggaaa
     6601 atatagataa tcttgaagat tattttataa cctatcttct ttataaagaa tctaaaacag
     6661 tatctcaaat tagtaaaatt agaaatattt caagtactga agcaagtgag caacttatac
     6721 aagcaaagtt aaaaataaag gaaatgcaaa aagatgattt tgaggcatct aaggatattt
     6781 tggataaatt tctagaacta gataaaatta agagacttga ttttatggat agcttagatg
     6841 acgaaaaaat ggtatacttt aagagaaagg ttttcaaaag aatccttgta gaaaaaaatg
     6901 ctgaagattt aattgtttta atttgggcga caggtgagct aaaagatgat aggtttttga
     6961 aattgcttca tcagctcaca aaccacagac actcagatat tagaagaata acttattcag
     7021 caatcagaaa gattgaatca ccaagtagta gagaagtact gcaaaaaggt ctctacgaca
     7081 aaaatgcaca gactaggcag tactgtgcaa aagctctttc aaaattggga gatgaaaaca
     7141 gtttaaagat tttacagcag ttgaaagaca agaataagaa ttttgaaaaa gaatatgtgc
     7201 ttagagctta tgacgaagcc attaagtcat tggaaaatgc aaaaaatttg aaaaagtaat
     7261 tttttattaa agcaatttgt ttacttaatg atgtatttct aaagtttaac aaggtaataa
     7321 ataaattaat gggaggggtt tcaaatgata gtaagacgtt gtgcaggtgg agtagtgttt
     7381 tatgctaata aagtacttat tgtaaaaaat gataggggtg aatggacact tccaaaagga
     7441 aagatactag gagggggact tccatatgag agtgctgttc aaagagttaa agtagaaact
     7501 ggcatagatg ctaaaatgat agatgtggct ggcgatacta tgtatgaatt tttctcaaga
     7561 agtagacaac aagaagtttg taatgcaata atgtggtatg ttatggaagc ttgtaacact
     7621 gattgtgtac ttgctcctga atttcaagaa ggtggatttt ataaagtaaa ggatgcttta
     7681 gaaatgttat cacatcataa agagcaagct ttagtagaag tatcttataa aaagtttaag
     7741 gaattaaaga aattagtttc agagggcgat gatgttccag caaattaatt gtgaatttag
     7801 aggattctga ggaagggata gccccttcct tttttaattg aagcagtttt aagcgtaaat
     7861 tatatagtct ctttaaacta tgatttatca atttagagat ttagtagttt atatttaaaa
     7921 tcagtaagat atgtttaaaa taaaaattaa aaagtataca attttattat ataattgtct
     7981 tatgatgttg attaaagaat tattaaaagt ccatttattt ttaaatagaa taattttata
     8041 aatatatatt tgatgtgtat attagaaaat gattaaaata ggaggaaata caatgagcaa
     8101 ttacaggaca ttacatgaat ttggaacgga tgaaataacg attgaaaagt ctgtttttat
     8161 tggatatgct aagcctataa agagtgaaga agaagcatta gagtttataa atgaaataaa
     8221 gaaaaagcat aaagatgcaa ctcacaatgt gtgggcttat actgtaggta aaaatatgaa
     8281 tatacaaaga tatagtgatg atggagaacc acaaggaaca gctgggatac ctacattaga
     8341 ggttataaaa aaagaagatt tgagagatgt agcggttgtt gttactaggt attttggagg
     8401 aacaaaatta ggtgcaggtg gtctagttag agcttataca aaaggtgcta aactgggact
     8461 tgaagcaggt aagataattt ataaggtaat gtatcaagaa gttaaggtta agatagatta
     8521 tactcaactt ggaaaagtgc aaaatgagtt aatgaatttg gggtatttta taaaagatac
     8581 tgtatatgag gataatgttg aaatagtagt atattcaaga ttagaagatg tagagaaatt
     8641 atcagaaaaa atgattgata taacaagtgg aacaggaaaa atagttttag gagaggaatt
     8701 ttatttgtct gaacaaaatg gagaaatttt aatataatct aaaaggttat tagaatagtt
     8761 aatacctcaa atagccaatt agtattttta agtgaaaagc cattaaattg atttaatcta
     8821 aatcaattta atggcttttt atcggatttt gcacagaatc ctttttttac ttcaaataaa
     8881 ccgactaaaa gtaaaaatat cccaaatatt ttttttaata tatctgaaga cagataatta
     8941 gctaatagtg aaccaaatac agctcctaaa accccaaaca cagctatagg tataaccaat
     9001 ttaaaaatta catttttatt ttttatatgt atagcaagtg ctataagtgt catagggata
     9061 gatgaaagta aatttacgct ttgtgctatt ttagtgtcta tgccagcaag taaaacgagt
     9121 gcaggtataa ggattgttcc tccacccatt cccattcctc caataattcc tgcaaaaaat
     9181 ccaattagag caaataacat taaaacacca tccttaatgc tgcaattatc ataattattc
     9241 caaaagaaat tctaagaaac tttccagtaa ccttatttag taatttagct cctataatac
     9301 caccaagaat acttccaact gcaactttaa ttgttaagtt aacatcataa gtacctttag
     9361 atacatataa aacagaactt gctgtcgtta agaatattat tatagaaaga gcagttgcat
     9421 gagatttatg ttcttctacc ttaacgatgt catttaatat tggaactaaa agagtacctc
     9481 ctccagaacc aaaaactcca tttataaatc ctgtaaaaac tccaattatc gtatttttta
     9541 agttaaaatc aaagtatttt ttctttaaat catcagtttt tttcataaaa atcaccttct
     9601 gaaatagttt taccaaaata ggcaaattat atttatataa aataagatta aatataatct
     9661 ttcatatttg tttaattttg cataaaaaaa aacatattaa aacaacatat taatatatgg
     9721 tataatatat taaaacaaag tagcaaattt gagaggagat aacaatgtca aatataaaaa
     9781 tacaaataga caaaactgta gaatggctta taaataaggt taatgaggca aatgcaaaag
     9841 gattaattgt tggtgtatct ggaggaattg attcggcagt agttgctaat ttaattaaaa
     9901 aggcatttcc agaaaactct atgggagtta taatgagcat aaaaagtaat cctcaagata
     9961 gagaagatgc acttaaggtt atagagggat gtgatataga ataccttgat ttagacctta
    10021 tagaaccaca aagtgctata ttagacatgg tagtaggaaa tttaaaagat aaacatttat
    10081 atagagaaga atatttaaag atgacagatg caaacttaag agcaagagta agaatgagta
    10141 caatttatac aattgctaac aatctaggat atctggttgt tggtacagac aatgctgcag
    10201 aaatacacac aggatatttt actaaatttg gtgatggtgg agttgatatt ttacctattg
    10261 caaatttaac taaaggagag gtgtatgaat gggcaaaaga gcttggtgtt catgaagatt
    10321 taataaacaa agctccttca gcaggtcttt gggaaggtca aacagatgaa gatgaaatgg
    10381 gaactactta caatatgata gatgctgtgt tagaaggtag attggatgaa gttcctaaaa
    10441 gagaccaaga gataattgaa agacttcata gattaagtga acacaagaga aaaacgcctg
    10501 cacaacctcc taagttctaa tttactattt ataagatttg tgataaaatt tacaagtgat
    10561 gaaaattaga ataattaatt gtacgtatta aggagtggaa atatgggacg tataggtaat
    10621 ataattaata gaaaaggtaa acaagatgct caaagagcaa agatatttac taaacatgct
    10681 agagcaatag cagttgcagc taaagaaggt ggagcagatc cagagtacaa tgcagcgtta
    10741 aaaacagcta tagaaaaagc taaggcagat aatatgccaa atgataatat agatagagct
    10801 atcgctaaag gtgctggagc aggtgctgga gaagactatg aaactatagt ttatgaagga
    10861 tatggacctg gtggagttgc tgttatagta gaaactttaa ctgacaacaa aaacagaact
    10921 gctggtaacg taagatatta ttttgacaaa aacggaggaa acttaggaac tagtggatgt
    10981 gtgtctttta tgtttgataa aaaaggacaa atattagttg gtttaggaga tggagtttca
    11041 gaagaagaat tgatggatgt tgcattagaa gcaggagctg aagatttcat aacagaagaa
    11101 gatggttatg aaataataac tacaccagaa gatttttcta gtgttcgtga tgagttaaaa
    11161 gctaaaggat atgagtttat atctgctgat gtgaagatga tacctcaaac tacaactgta
    11221 ttgactgaag aatctcattt aaaaatgatg aataaattag ttgatatgct tgaagaagat
    11281 gatgatgttc aagatatata tcataactgg gaagtagaat aaatagtata agcatattga
    11341 aatttataag tattgaatca attttaacat gtgataaatt ttatagaata agaaacacta
    11401 aaatagacat acgcacctct aacctatatt tgcataataa tatgtaagca taggtaaggg
    11461 gtgtttttat atttataggt taagaaattt aaagtaaatt ttgagctgga ctcgattgtc
    11521 tataaataat tgatgggtat ataacttgtt gctgaatacc atttatacgc ccagttactg
    11581 tatcaataat catcctaaga ctacgttctg ctgtctcttc catttttaaa tctataactg
    11641 tcaaaggagg aatacttctt gccattagtc gtgaaggtcc attacttgct gcaaatattt
    11701 gaatatcctc tccaattaca attcccgatt catgaaatgc attcatcata ccaagagcta
    11761 ccacatcatg attacaaaaa attacttttt ttagtgaatt ttttgcaagc attttttgag
    11821 caatatcata tccactatct attttattag aacaataata tatagaatct gatagatgta
    11881 ttccatgttc tttacatgtt tttaaaaaag atttacccct taaagtcata catgtaaagg
    11941 aagttgggtt gaatacaaca gcaatatcat ttttaccatt ttgaatagca tgtaaagctg
    12001 ctatacgacc agtttcctca ttatcaatag atacacttga aaaaccaggt atttcacgat
    12061 ttacaacaac tacaggaatt ttaggtttat tttttactag atattctata tcatcagcat
    12121 tgatacctgt taaaacaatt ccatcataat aattatttgc aaaaaagtat tcatcatcac
    12181 atagatgacc aactttgtaa ggtcgtatga tgatatttat gtttaaatgt tctaaagaaa
    12241 ttatgtggtt tattgcttga agtatattgg ttgatgccaa ctcaaatccc tcatcctgcc
    12301 aataaaaagc tattttaata ttattatgtt taataggagt agagcgttta tatccaagct
    12361 tatcagctga atttaatata agctcttgtg tatgtagcgg aatcctgcgt tcatttcctt
    12421 taccacttaa gacgtacgag acagttgctg aagaaagacc acagtctttt gcaatatctt
    12481 tgattgttgc catgataaaa tcctccttaa atagttgctt aaatttgctt ataccaataa
    12541 tcttaacata tattcaacta aagaacaata ttctacaagt tgcatatttt acaggattag
    12601 aataaataaa tttaaaaaaa cttcttactt atgttgacat aatttagttt ataggattat
    12661 aatatgctta aataagctta taattaactt aaattaagca aagttaagca gggacatgca
    12721 atgctagctt gcaatgtgac tgtaaagggg gaaaaggtat gagtaaggta aatatatttg
    12781 aagttggtcc tagagatgga tttcaaaacc tgaaagagtt tttagaaact gataaaaagc
    12841 ttaagattat tgatggtctt attgcttcag ggattaaaca acttgaaatt agttcatttg
    12901 taagcccaaa agcaatacct cagatgaaag atgcaaaaga agtagcaact tattgtgtag
    12961 aaaaatatcc tgatgtgaaa ctttatgcat tagttccaaa tttatacggt gctaaagttg
    13021 catgggagtg tggtataaga gatatatcct atgttatatc tttgagtgaa actcataata
    13081 aaaaaaatat aaatcgtacc catgaagagt ctattgatga gcttaaaaaa ataatgaata
    13141 cttatccaga catgaatata tgtattggaa tggctactgt atttggatgt ccttttgaag
    13201 gtttaccaaa aattgaagat gccataagat ttgctaaatg cctttgggaa aacggtatca
    13261 gggagataaa tctagcagat acaataggga ttgctgaccc taagcaagta agagaaacta
    13321 ttagtgcatt agttgaagct ttaccagagt gcagatggca agtacatatt catgatacta
    13381 gaaatatggg aatggttaat actcttgtgg ctattgaatc tggaattact actgttcagt
    13441 ctacacttgg aggattagga ggatgcccat ttgctcctgg agcatctggc aatactgcaa
    13501 ctgaagattt agtatatatg cttgaacgta tggggtatga tacaggagtt gattttgtta
    13561 aacttcttga attatcaaag tatcagaaga gttttgtaaa tggagtatat agtggtcatc
    13621 atgtaaatat aaagtctgat agttgtaatt gttagttcta ttgattaatc gattaaaaaa
    13681 tatgttgatt aaaaaaatat gctgattggt aaaatagatt tattgataaa aaatataaat
    13741 tataaaatac attatctaat aaaacaaata aaaaaattga gggggattag tatggatatt
    13801 atagctttac ttttattttt aggagttatt attattgcat ttgtacgtaa gaataatgca
    13861 ggtattttgg ctctagctat tggtgtcata gctgttagaa tttttggaat ggatgataaa
    13921 gaactttatg gagctataag caactcaatg tttactacac ttgttggtat aacactactc
    13981 tttgcaatag tgaactcaac aggagcatta aagcttgttg cagaaaaaat tgtagctgct
    14041 tctggtaaac gtgtttggct tatacctata gcagttttta tagcaggatt tgttgttgct
    14101 ggtgttggac ctggggcaat tccagcactt gcaattatcc cagcaatggc tgttcctgta
    14161 gccataacag ttggttataa ccctgtaatg cttgcactta taggtgaatg tggattaatg
    14221 gcaggtcgta tgactcctat tactccagaa ggtcaaatta taacaaccgt tgcagaaggg
    14281 gttggaattg aaaatgttat gcctactatc ttagcctgtc aaactctttc tacaattgta
    14341 ttttcaatag tattgtttat aattttcaaa ggatataaac ttaaaaaacc tataaatgtt
    14401 atagatagta agcaaataga aaaattcaat ataaagcaga taatttctct aataagcata
    14461 gtagttatga ttgcttttat cataatcttt gatatgaatg ttgggcttgc agcttttgca
    14521 gtttcagctg tacttatttt tttcaatatt gctgatgaag gagaatgtat caaatcaatt
    14581 ccatggagca cgatagtaat ggttcttgga gtaggtgcaa tgatgacaat cgtagatgca
    14641 gcaggaggta ttgacttgat gagtaatgca ttatcatcac tgatgaatag taaaacagct
    14701 actccaatta tgagtatttc agcaggactt atgtctatgg ttagttctgc tttagcagta
    14761 gtttatccaa ctatgatgcc tatgtgtgtt gatatagcta aggcagttgg aggagtagac
    14821 ccactagcac ttattgctgc agttggtgtt ggaggttctc ttgctggtgt aagcccactt
    14881 tctactggtg gggcattgat tttagcagct atgggttcaa gtaagaaaga ctttaataag
    14941 gaggaacaaa ataaggtttt tgtacagctt ttcattatga gtgcagttgg cttattggtt
    15001 attgcagtag tatcaatatt atcatttaat gcaataacac atttaatgaa ctaattagaa
    15061 taactgaaaa taaatcatta aatttaactt ttaatattaa tcaatataat ataatttgga
    15121 ggggaagtta tgaaaaaagg agcattaagc aatataaagg tacttgacct tacaagagtt
    15181 cttgcaggtc catattgtac aatgatgttg gcggattttg gagctgatgt tataaaaata
    15241 gagatgccaa gaaaaggtga tgatacaaga ggttttggac cttttgtaaa tggagagagt
    15301 ttatactatg caaatataaa tagaaataaa aaaggtatca ctctaaacct taaatcagaa
    15361 gaaggaaaaa atatattttt agaaatggta aaacatgctg atgttgtagt agaaaattac
    15421 agaccaggtg taatggaaaa gttaggtctg ggttatgatg agttggctaa agttaacaat
    15481 caaataattt atggagctgt atcaggattt ggatgttatg gaccatattc agaacgtcca
    15541 ggttatgata ttattgcaca ggcaatgggt ggtcttatga gtataacagg gcaagaagga
    15601 ggagaaccaa cgagagttgg taatgctatg ggtgatgtac taggtggaat gaatttaact
    15661 attggtatac tcacagcact caacgctaga aaaataatag gaaaaggaca aagagtagat
    15721 gtttccctcg ttgattctgt tgtatcgtct ctcgaaactg gtatacagag atactttgat
    15781 tcaggacaac ctgaacttat gggtaataga tatgcttcag cctatcctta tgactctttt
    15841 aaggcaagtg atggaagttt tgtaataggc tgtggtaatg acaaactttt tgaaaagtta
    15901 tgtaaaaaag ttctttttag agaagacctt ttaatagatg agagatttaa aacaaatctt
    15961 aatcgttgtg aacattacaa ggctcttaaa ccagaaatag aaaaatggtc tgttaagcat
    16021 acaataaaag aagcagttga cttaatatca gaggctggag ttcctgctgc tcctatctat
    16081 aatcttgatt gtgttacaac agataaacat atagcaaaag caagagaaat gattgtagat
    16141 attagtcatc ctattatagg aaatatgaaa gtcaatggaa atccagttaa gttgatgaca
    16201 acaaaagcta aaattgatat gccagcacca tctctagggg aaagtaacga ggagatttat
    16261 aatggttggc ttggttttga tagtgataaa ataaaagatt taaaagaaaa tggagttatt
    16321 tgattaaaaa atagctaaac acacttgtga ctttagtcat gggaggttca gttagaataa
    16381 tatcaaaaaa tattaaatca atactgtaaa tcaaatatag aattgtcaga agcatttaag
    16441 atgggaaaag ctacttagac attgagtctt tgtggctttt cctattttca ttaagaattt
    16501 tatattatca gaatagatat gtactaaact aaagtattaa caatttaaat taatagaaaa
    16561 taaactacct aattatgttg aataaagact actttagtga aaacaaactt atagagatgc
    16621 tatttaaatt attttaatgg aaatatcaaa aatgagactg cattctcaaa aatgagactg
    16681 cgttttttaa gtattttctc gaaaatgaga caaaaatatt gttttatggg ttttattgaa
    16741 gaaaaattct tctgtgaatt acaatttaat caattttaaa gttgaaaatt attaaaaatt
    16801 tagtgaaatt tatgtatatt tcgtttattt tattaaaaat aacaattttt tactaaaaat
    16861 aaagaacttc aatggtgatt ggcatagtac ttgctatttt atattaacaa gaaatgtaga
    16921 aaaaataaca atgtataatg gggggaatat gatggaatct gtaatggaaa atttgaacaa
    16981 tcttaaggtg gaggttaagg acaaagtctg tgttattact ataaatagac ctaaagcttt
    17041 aaatgctctt aatagtgaca ctcttaggga actatcacaa gttattgatg tagtatcaga
    17101 aaatgaggca atattaggag ttattattac tggagaaggt aaagtttttg tagccggtgc
    17161 tgatatacgc cagatgcaaa actataaaag tgaggaaggc agaaaatatg caggttatgc
    17221 tcagggaatt tttgataaaa ttgaagctct tgagaaaaca gtaatagcag cagttaatgg
    17281 atatgcatta ggtggtggat gtgaacttgc tatgagttgt gatattagaa ttgcatcaga
    17341 aaaggctata tttggacaac ctgaagttaa tttaggagtt attccatgtt ttggtggaac
    17401 tcaaagatta tcaagacttg ttggaactgg aatagctaaa gagttaattt ttacggggag
    17461 acaagtaaat gcagaagaag ctaaatctat tggacttatt aataaagtgg ttccaagtga
    17521 tttactttta gaagagtcta tgaagatgat gaaccaaata gttgaaaaag cgccaatagc
    17581 aataagatat gcaaaagttg ttattaacaa agggatagat atggatttaa agaatgctct
    17641 tgaactagag aaagatgttg caggtttaac ttttgctaca agagataagc aagaaggaat
    17701 gaatgcattt atagaaaaga gaaagccagt ttttgaaaat aaatagctat tataactaaa
    17761 attggaacaa tgtagaatat attcaaaatc taaatttcgt gaaaaaatac atatagttta
    17821 attttaaaag gagttaattt atgaatacgg gagtaattat aagtttagta gggattatca
    17881 tagctctatc tcttctagtt gtaatggtta tgaagggaat aaatattttt attatagcaa
    17941 ttgtatgttc atcattagtt gctataacag gaagcctaaa tgtgtatgat gcacttaaag
    18001 tggattatat gacagggttt gttggatttc ttcaggcaaa tttctttatt ttcctaacag
    18061 ggacattaat gggaaaaata atggagatta ctggtggagc aaaatctatt gctaagatga
    18121 ttgtaagatg gattgggaag gataaagcac tactttctat tccaatagct tgtggaatat
    18181 tagcctatgg tggagtaagt gttttcgttg taagttttgc agtgtttccg atagctttag
    18241 aaatttttaa agaagctgat ttgccacgaa gatttatacc agcagctttg acttttggat
    18301 gctcaacttt tgctatggtt gcaccagggg caccacaaat tcagaatatt gttccagcat
    18361 caacattagg gacagatatt atggctggag cagtaaatgg ttttatatca tgtgcagtaa
    18421 tgtttatagt tggaagtaca attttgtaca aaatggtttc aaaagaaaaa gccaatggtg
    18481 gtcattttat agcacatgaa tcagatatat ttgatgaagc agaagcaact aattctaaga
    18541 gagaaaatgg tcccaatggt gtaattgcct taattccatt gatagtgtcg attttaataa
    18601 ttaatgtaaa agttaatgga aaagcgatag taccaattga ggtgggagta tttattggag
    18661 ctttattagt atatttattg ttaaataaat atcaagataa tactaaaata gttggacatg
    18721 ttggtgatgc atgtaaaact acagtagtag caatatgtaa tacttgtgct gtagttgcat
    18781 ttggttcagt agtaaaatca gcagttggtt ttgacttcat agttaatgct atgactaata
    18841 ttcctggtcc tcctattgca ggggctgcag ttggaactac agttattgca ggtatttgtg
    18901 ggtcagcttc aggtggacta ggaattgcag ctccacttct tggaccagta tttttagcca
    18961 agggtgtttc cgctgcagca ttaactagga ctatggcaat ttcatcagca gcattagatt
    19021 cacttccaca caatggatat attgttactg taacaaatgg tctttgtaaa gaaacacata
    19081 aggatgccta tatgccaata ttctgggtaa ctgttttaac cccatttatt ggaaccatag
    19141 tagcagtcct actgtttaca atgttcccgg gattgccata ggagaaaaga taaaaggtat
    19201 aaattatatt ttgatattat aaaatcaatt ttcaaaatat atacaataaa tttcatgttt
    19261 taatactgta aatatgtatt taaaataaca aattgaccat ataaggagga attgaaagtg
    19321 ttaaataaac cattagaggg agttaaggtt attgacctaa cgtattttgt agcaggtcca
    19381 gggacttcta aaatattagc tgactggggt gctgacgtta ttaaggtaga gccaagcttt
    19441 ggagacccag gaagaaagac aggggcaaca atgacaatgc caatagatga ttataataat
    19501 cctttttaca gcacatataa ttcaaataaa agaggtttgt ctataaatct taagagtgaa
    19561 actggaattg aaattatgga taagctttta agcgaagcaa acgtatttgt ttcaagttat
    19621 agaacaggag cattaaaaag actaggtctt gattatgaat cattaagtaa aaagcatcca
    19681 caccttatct ggggtcagat aaatggtttt ggcgattttg gacctgctaa ggataatgca
    19741 ggatttgata cagttgcctt ttgggcaaga agtggggcta tgcttgatat agcagaaaaa
    19801 gatacaagtc caataaatcc acctatagcc tttggagatg caacaacttc ttgtagttta
    19861 tctggaggaa tatgtgcagc tttgtaccag caagctaaga ctggaaaagg acaaaaagta
    19921 atggcatctt tatttgggca agcaatatgg aatgcatcat ctttattagc atctacacag
    19981 tttagtgatg agtatccaaa aactagaaaa aatgctattt caccagtaat aaattcttat
    20041 cgttgtaagg atggaaagtg gatattctta agtattttag agcatgaaag atattttgag
    20101 gcactttgta gattatttgg aagagaagac ttaataaaag aagagaaatt tgcatcttca
    20161 gtagaaagta agaaaaatgc tacagaactt attaatattt tagatggtga atttgcaaag
    20221 tttactcaaa atgagatggt tgaaaaactt actgctgctg atatagctca tgaaaaaatt
    20281 caacatgtta atgatgtagt gactgatgaa caggctattg ctaataacta tatatatgaa
    20341 catacaagta aagatggaaa taagacaatg cttgcatcta ctccagttaa atttggaaat
    20401 attgaagtta acatgacttg tgatgctcca cttataggag aacattcaga tgagatattg
    20461 agagaacttg gatacagtga ttctgatata gagactctaa ttgaatctgg aattgtaaca
    20521 atgaataaag aagcatgtga aatatgttaa taaacatgtg aagtatgtta ataaatagga
    20581 gggaatttta tggattttag attaacagaa gcacaattaa tgcttcaaag agtagcaaaa
    20641 gaatttgcag agaatgaaat agcaccaata gctgctgaaa cagataaaac aggtattttt
    20701 ccaagggagc tttttagcaa aatggctaaa ataggattta atggaattgg aacacctgta
    20761 gagtatagtg gttctggtgg ggcagatata gaaaaggtaa ttgttgttac tgagattgcc
    20821 aaaaaatgtg cagcatctgc agcaatatta tcaatacaca caatatatgc tcaagctatt
    20881 ttgaaatttg gtactgagga acaaaagaaa aaataccttc caatgatggc tgaaggtggg
    20941 tgtgtaggtg cttttgcctt aaccgaacct aatgctggtt cagatgctgc gagagctgca
    21001 acaactgcaa ttattgatga agaaactgat gaatatgttt taaatggaac taagtgtttt
    21061 atttctggtg gaggtcaagc agaatcatta ataatatttg cattgacaga cccatcaaag
    21121 ggaataaagg gaatgtctgc tatcattgtc gacaaaggta ctccaggttt ttcaatagga
    21181 aaaattgaag aaaaaatggg tattcatggt tcagaaactg cagagttaat atttgataac
    21241 tgtagagttc caaagtctaa cttattagga aaagaaggta aaggatttaa tatagctatg
    21301 acttgtttag atggagcaag aattggagtt ggagctcaag ctgtaggaat tgctgaaggg
    21361 gctttagaag aaagtatcaa atattctaaa gaaagagtcc agtttggcaa gccaatttca
    21421 gctttgcaag gtattcaatg gtatatagca gatatggcga caatggtgga atcagcaaaa
    21481 ttattagtat attatgctgc agatttgaaa gctagaggag aaaaacacac taaagaagct
    21541 gctatggcaa aatacaatgc gtcacgtact gcaagagagg ttacaaattt agcccttcaa
    21601 atccatggtg gatatggata tatgaaggat tatccattag agagaatgta cagagatgcc
    21661 aaaataacag aaatttacga aggtacttca gaaatacaca aagttgttat ttcaagagca
    21721 gtactaggat aggaggaaaa gaatgaatat agttgtttgc ttaaagcaag ttccagatac
    21781 taatgaagtt aaaataaata aagagacagg tactctaata agagatggtg taccaagtat
    21841 aatcaatcca gatgatagaa atgctttaga agaagctcta aaaatgaaag atgaactagg
    21901 agcagtgatt aaggttataa gcatgggacc tcctcaagct aaaagtgctt taaaagaagc
    21961 tttggcaatg ggagctgatg aagcttattt aatctcagat agagcttttg gtggttcaga
    22021 tacttgggct acatctacaa ttattgctgc tgctatagag aaggttggaa agtatgatgt
    22081 aatattctgc ggaagacaag ctatagatgg agatacagct caggttggac cagaggttgc
    22141 agaattttta gggataccac aagttactta tgcgaaagaa gtaaaggttc aagatgacaa
    22201 attactagta actcgttata cagagacagg ggattattta atagaagcaa agatgccagt
    22261 tttattaact gctataaaag agttaaacaa tccaagatat ccaagtgtca agggaatctt
    22321 agaagcttat aataatggag atgcaaagat tactgtatta acacttgcag atttagatgt
    22381 tgatacaact caaattggat taaagggttc acctacaaac gtttataagt cttttgtacc
    22441 agttaaagac aagcataatg aaataataga aggtataaat aaaaaagaaa aagctgagaa
    22501 actaatagaa atactattcg atttgaaact agtataggag tgttaagatg atgagagcaa
    22561 aagttaatca aggtataaat ttaaatgatt ataatggtgt ttgggtaata ggagagcaaa
    22621 gggaaggtaa aattaatcct gttacgatag aacttattgg tgaaggaaga aagcttgctg
    22681 accaattagg aaaagagctt gcagttgtga tagctggata tgaagtagag aaagaagtga
    22741 aggaattact tcactatagt gttgataaag tatattacat caatgaccct cttcttaaag
    22801 atttcactac tgatggatat gcaatttcaa ttgcaaattt aatcgaaaga aaaaagccag
    22861 aggttgtatt ggttggtgct acttctatag gaagagatat agcgcctaga attgcaggta
    22921 aggttggaac aggtctaact gctgactgta caaaacttga aatagattca acagataata
    22981 agttattaca aacaagacca gcatttggtg gaaatttaat ggccactatt gtttgtccaa
    23041 aaaatagacc acagatgtca acagttcgtc caggagttat ggcaaaagct gtaagaaacg
    23101 aatcagagac tggaatttta gaagttgtta ctccagaact tactgaaaaa atgattagaa
    23161 ctagattagt agagatactt ccacaagaaa agaaatctgt aaatctaact gatgctagaa
    23221 taatagtatc agggggaaga ggattaaaaa gagcagaagg atttgagctt ataaaagaat
    23281 tggctgataa attgggggca gagattggtg cttcaagagc agcagtagat tcagggtgga
    23341 tagaacattc tcatcaagtg ggacaaacag gaactactgt tagaccagaa ctatacattg
    23401 catgtggaat atctggagca atacaacatt tagcaggtat gagtgattct aaatatatag
    23461 ttgctataaa taaagatgca aaagccccta tttttagtat atgtgactat ggaatagtag
    23521 gagatttata tgaaattata cctgaaatga tagaatcatt aaataggtaa cttttagaaa
    23581 acattgaata tgtagaatga taagtcttat aatattgtat tgtaagactt attatttttg
    23641 ctgaaggtta tagtggggag atgaaacaat gaaagagcag tggtataagg atatatttgc
    23701 aagagtatta tctatgactg atgatggttt tattgttgta aatacaagtg gagtaataat
    23761 agatattaat gataaatatt gtgacttttt aggaaaagaa agaaaagata taataggaca
    23821 aaatatacaa agtattatac caaatacaaa aatgctagat gttatgaaaa ataagtattg
    23881 tgaagaagga gctatacatc attattcagg tggaaataca aaagaaaaaa gtgtaatagt
    23941 aagtaggtct tatgtggaaa atgacaatgg agaagtagtt gcaggagtag ctcaagttaa
    24001 atttagattg caatcatttg atgtagctaa aaagttaatg tcagaatata tggagctcca
    24061 atattataaa gagcagttta aggataactg tggttttgac aagctcatag gtgaaaatag
    24121 agacttcata gaacttaaga aaacaggtgt gaaagcttct aaaacaaact ttccagtact
    24181 tttaacgggt gaaacaggca caggtaaaga agtttttgct agagcaattc acaataatag
    24241 cagtaggtca gataaaccta tggttagtat aaattgcgct gcaataccag aagaattatt
    24301 agagtcagag ctatttggct atgacgaagg agcttttaca ggagcaaaaa agggtggtaa
    24361 aaaaggtaaa tttttagttg cgaataatgg tacaatcttt ttagacgaga taggtgacat
    24421 gcctttgacc atgcaagcaa aacttttaag agttcttcag gaaagtgaga tagaaccagt
    24481 gggaggtctt aaaactataa agatagatgt aagagtaatc tctgccacaa gaaagaattt
    24541 aagtaaaatg gttgaagagg gattatttag agaagaccta tactatagat taaatgtaat
    24601 aaacattcat atgatggaat tgaaggacag gcaagatgat attttattgc tagcaaatta
    24661 tatattaaac aaattaaatg ttgaatacaa ggaattgaag gttttaagtg ataaggttaa
    24721 aaattgtttt attaactata cttggccggg aaatataaga gaactacaaa atgtaataaa
    24781 aagtgcctat gctgtaagtg atgatatggt cattatgatg tgtgaccttc catctaagat
    24841 ggataatatc agcagagtag ctcaatgtaa tgtggatagc aattgttcaa ttcatgaaat
    24901 ggttgagaat tatgaaaaga gcttaattat tgatgtgtta aggaagtata attggaaatg
    24961 ttctaaggca gcagaggtta tgggaataca caagagtctt ttatataaga aaataaaaaa
    25021 gtatgaaatt gaattaaata attaagataa cttgaattaa ggcaatgcta aaaaagcgcc
    25081 cttattcaaa gcgaactgta taaactgttt tatgcagtta aaacgtgtaa aagtagatgt
    25141 gaagtactaa tgtcaatcat tagtactttt ttattatatt tataattact atctatattt
    25201 cagtatcttt tgatatctaa gattattatg tttaatttag tatataacaa aaccctctta
    25261 gttatggaca gttttttata ttttaactgt ctactaagag gggatagttt agtttaaatt
    25321 tagagtaaat tttatctagc atttataaaa aatattttga atagcttttt tgttatttag
    25381 tcataatttt ctacttcttt atcaagttct ttgccacaag aatcacactt atatatagca
    25441 ttgtatccgt aatcatattc atcaactatt ttcttttgtc taaatacacc accacactca
    25501 cataggaaac caatttttat aagtgtaacg ataaattgtg attttataaa ttttatatca
    25561 ttgcatttat cgttagtgta aaatgtataa atataagagt tatcgcactc atctcttaat
    25621 tcaaatcttt caatatatgt gttatttttt aattcctcaa attcttcttt agttaaatat
    25681 gatttttgtt gcaattcatg taaacgctta atttggtcca ttatttatat cctcccaata
    25741 attttagtat tttaagctta gataaggaat ctaagtttta ttaaatttat cccaaggaat
    25801 tatttaaatc atatttagtt ttattaatta caacatatat aaattacacg aaaataaaac
    25861 taattgtaaa aataatatcc tgtgaaaacc taaactacta accccttact aacccgaaaa
    25921 ctaaaaaaag aaaatatatc caaatataag tgatatataa ataaagatgt ttcatataaa
    25981 tttaacttta aatattctga caataatata tataaatata ttatagttaa aatataccac
    26041 atattttggg tattgtatac tggaattaga gtaattaatt tattttttaa catacaaatg
    26101 attttatgaa aaaaaacaag actcaaaaat ttttatattc tttgatagaa taaaattgag
    26161 agtaaattta ttgaatttta taaaatttat aaggggtgag tctaagatat gcatgaagat
    26221 aaaaatgata tatttgaatc aatagatact gaggattttg ataataaaac agaagaagaa
    26281 tgtttagagc tagtaagaaa aaatggattg aatttaatat acataaaaaa tcaaacagaa
    26341 gagatatgtc tagaggctgt tagacaaaat tgtaatgcaa taaaatgtgt tcagaatcag
    26401 acagaaaaaa tatgtataga agcagttaaa caagattgga gaatgttaga atttgtggaa
    26461 gagccaacag aagaaatttg catgttggct attaaccaag atggaacagc attaaaatat
    26521 atagaaaatc agacagagga gttatgctta agagccgttg aaaaaaatgg tgcagctttg
    26581 gagtatgtaa aagaacaaac agaagaaata tgtatagaag cagttagaaa cagcgaattt
    26641 ggattggcaa gagtgaaaaa tcaaacagaa aaaatatgta tggaagcagt taagcattgt
    26701 agctataatc taaaatgggt agaaaatcaa acagaagaaa tatgtatgga agcagtgaga
    26761 caaaatgggc ttgatttgaa atttgtaaaa aatcaaacag aaacaatctg tttaagagca
    26821 gtaagacaaa atggaatggc attagaattt gtaaaggaac agactgttgg tatatgctta
    26881 aaggcagtaa gacaatatgg aatggcatta aaatttgtaa aggaacaaac agaagaaatt
    26941 tgtacagaag ctataaaaca agataaaaga gcacttagtt ttgtaaaagg agataaggaa
    27001 aaatataagg ctctatatga taataatgaa ccatttgcaa agagatatgt tagaaatgta
    27061 atagaaaaag aaaacagagc attgattaaa aaaggagaag aaaatgcaaa aatcaaaatt
    27121 gcaggaaaac tttatgatag aggcatgtct tttgaagata tatcagatat agttgagatt
    27181 gaaataagca agttaaaagt gagtttaggt gttttttaaa gtaataaatg taggtgtttt
    27241 ttatgggaaa tagttaataa aaaaataatc gactagctct ttgtaggaca gcaaaattag
    27301 tgatataatt tgatgtataa taaattattt ttattagata agggggaatt aaagttgaag
    27361 attacagtat tataccaaac aagaagtgga aacacagaaa gggtcgctaa gctgatagaa
    27421 gagggcgcta aaaaggtgga tggagtagat gtaaagctta tgagacttga taatattgat
    27481 ttagattatt taaatgaaag taaagcagta atatttggaa cacctactta tttagcaaat
    27541 acaacttggg aagtaaaaaa atggtttgat gaagattcaa aaaaagttaa tttagctgga
    27601 aaattaggag ctgtttttgc tacatgtgat tatatatgtg gaggtccaga tgttgcgata
    27661 cttactatag taggacattt gatggttaaa gggatgcttg tgtattctgg aggtggttct
    27721 ttaggaaagc catttataca tttaggacat gtacattcaa ttgaaggtcc agagttacaa
    27781 gatgagaagg ctataatatt tggtgaaaga attgctacta aggcaaaaga actatttgct
    27841 taatttctat atgcatagta atgattagag taaatttagg gttttaatgg agatttgaag
    27901 taaaagttat aaaataaaaa attttccata aaattactca agatgaatta taatataatt
    27961 ataaataaat tatattatta aaatatttaa aatcaaacgt caatagcgag ggaagtttat
    28021 ggtatggcat atagaatatg agattttttc tgcaattata gttatttttt taatggtata
    28081 cttttttaga agtaaattta tacctacttt acaaaacaaa atatattgtg cacttttaat
    28141 attctcattt ttatttataa tttcaaatat tttaggttca ttttgtctaa ataatataga
    28201 caaaattcca atttttatta cttttttatt gaatcagata tacttattgt tattgccaat
    28261 accagcagca cttgtatcat tttatgttat ggctattatt tatcaagata taaggtatat
    28321 gaaaaaaaga ttattatttt tatctatacc acttataatt tgtataatct tatctattac
    28381 aaatcctttt acgcatattc tatttggatt gtcaaaagaa acaggttata tacgaggttg
    28441 tggatatgtt gcaacttttg catctttttt cttttatgca atatattcag catttttatc
    28501 ttttaaatat agaaaagcaa tgcatgaatc aaaaatatgg gctattagag gttttcttat
    28561 agtttcaatt gtggcaatta tcttacaggc aattttttta cagtatcttc ttacaggaac
    28621 tgcatgtgca tgtagtttac ttttgacata tctttctatg cagaatcgag gtcttattat
    28681 tgatgacctt actggtgtac tcaatagaca atcttttatt caagagctag atttgaatat
    28741 caatactgaa caatcaggat ttattattac aattgcgctt gatgatttta agtttatgaa
    28801 tgaaactttt ggaacaaaaa atggagatat tgcactaaaa gaagttggaa aatatttgat
    28861 tcagatacat gatgtaaacc atgtatatag aataggggga gatatatttt ctattgttgt
    28921 caatagtcaa ttaggtatag aaccagatga tattatcaga aagatagaag aaagatttaa
    28981 aaagccttgg ctagtagaag agataagttt taatttagca actagtattg ctgttgttta
    29041 ttacccagaa aatgcagaaa cgacagaaga tgtaattgtt gctatagatt tctctataca
    29101 tgaagcaaaa cgctctgagt caaggcgagt tgtttatgca gatgcgtcta taagtgaaaa
    29161 gataaagaag aaacatgtta ttaaaaattg tttaaaagaa gcattaaaaa atgatgggtt
    29221 tgaagtgtac taccaaccta tattttctaa tagcaggggc aggtttactt cagcagaggc
    29281 attacttagg ctagaacaca aagagttagg ttttatacct cctgatgaat ttattccaat
    29341 tgcagaacaa acaggattga ttaactcaat tggattagta gtctttgaga aggtatgtag
    29401 atttatagct agtgatgagt ttgaagattt aaaattggat aatatagctg tgaatctttc
    29461 tgtagtacaa tgtatgcaaa aaaacttagc tgatgattta ctttatctta tgaataaata
    29521 taatgtatca ccaactaaat ttaaacttga aattactgag actgtggcta tgggctcttt
    29581 taatataatt aaagaaacaa tggaaagatt aattgattta ggtgttaagt ttgcacttga
    29641 tgattttgga attgcttatt ctggagttac taatatgctt tctttaccat tttcagtaat
    29701 taagttagat aagagtttga tttggtctat gaatgaagat agtaggcata aacttacagt
    29761 tgagacgatt atagcactta taaataaatt gaatatgaag gctgtagcag aaggtgttga
    29821 aaccattaag tgtgctgaag atttaatagc tatggagtgt gaatatttgc aagggtatta
    29881 tttttctaaa ccagttccag aagatgtttt taaaaatttg ttgaaagaaa atacttttac
    29941 aagttaacac cctttatttt tttaattaca actttgttca ttgacactgc cagcgatata
    30001 ttataaaatt aacacaataa taaatattaa atttattata catattatta atattaatta
    30061 atggagtaaa gatttattat atattttatt taattatcct aaagtgagaa aggaagatgt
    30121 gacatgaata aaaagaaaat attagtgcca attgatggga cagaaagaag tatgcattct
    30181 ttggaattta taaaaggaat atttaaaaaa gatgaagttg aagtagaaat aatgaatgta
    30241 aaagaactag tatttattga tggcatatcc ctggctgaag agataaaaaa ttctgaaaat
    30301 ttaggaagaa gaatcttgga taaagcagca gagataatgg gagagtatga tgttaaagta
    30361 cattttactt ttggatatcc aggagatgag ataattagaa aagcaaaaga agataatgta
    30421 gattttatag ttatgacaaa atctactaaa aaaggtctta caagaatgat aggttcagta
    30481 acagcaagtg tagttaaaca agcaaaatgt atagtgatga ttgtaccgga gtagaattag
    30541 aaaggtattt gaaatatgaa taaaaagata gtagcagttt gtgaaagtga aagaagttta
    30601 aattctctta agacagctgc aaatgattta ggatttgatt tagtttatga aattcaagaa
    30661 aatgataata taaaaaatga actttctatt tatgatatag aagatgcatc catagtgtta
    30721 tttgttacaa gtgattctat agaaagcata gagaagattg atagatttat agatagagaa
    30781 ttttatgaag ttgatccaaa gtatataata gaggatgcaa aatctatagt aagtgaaatt
    30841 ttaatagact taaattaatc gtgggtgtcg cactaagaat agagcgacgc ctttttatat
    30901 attttacaca agaaataaag aggttgtatc aaaatataga taagagtctt tttcattgtt
    30961 gaaatgatga aaatatattt gatttcatac aaaaaagagt acctcataac taaaaagttc
    31021 actttgagac actccatttt tatatgttag ttgcatttaa ggtaaaatag aagtttaatc
    31081 atgttgaaat aactgaatta catcttctaa atgattgtac attgatgtat aacctaagta
    31141 agagtcttta ctatcaaatg caatcattat cttgttatgg tggctaatca tatccttgag
    31201 agaatttcta tatttaaaac caaagtattg gttagaagaa actgttttag gtattaactc
    31261 ataatatttt ataaaaattg gattatgaga agctaaaatt atctctttgt gaaattgcaa
    31321 gtcagcatca taacattctt ctatcaaacc atgagaaacc ttatccaatt cctctattga
    31381 ctttaggtaa gtatatttta ttttttgaag ttctttagat gtagcattta aaattgctag
    31441 tttagtagct tcgctttcga taagttgtct tgctcttaaa gattctacat aaaattcttg
    31501 tatgttatga tttttataga tttcaccatc atcaaatagg tcaacttttt taaatccttc
    31561 aactatagga cttgaataag gcacagctct aagtattcct tccttacaga gtatatttat
    31621 ggcatttctt acaggtattc tacttatgtt aaataggtca gcaatctttc tctcacttgg
    31681 taatttacta ttaattttaa aagttccatt tagaatgttt aatttaatat aatcaataat
    31741 tttttcagtg gcatttaagc ttttcataaa aactcctaaa catataattt ttttatgatt
    31801 gtattatatc atagttaaga gttaaatcta cttttttata atatgtataa gacaggatag
    31861 aggccaaaat agctataata gccaagataa agtatccata ttgtattgaa gtatattccg
    31921 atattttacc aactatcatt ggaaatacag acattcctat agcataaatt gcttgaaaaa
    31981 aacccattgc agtagatttc tgatgttttg gtacattttt catagactcg ctagtaaggt
    32041 aagaaaggag tatacctgta gatatacctg gtagtacttg tagtacaaat attatattaa
    32101 tattttttat tgttggaact aaaaagcaat aggctgaaac taaaataaaa actaaaggaa
    32161 tccaaaattt tggtccatac ttagtacaaa atttagtaga agcaaagcca gaagaagcaa
    32221 cagcacacac catgtaaaat atagaagata gacctatgag ggtagaacta gaacctaagt
    32281 ttttaagaat ttgagtagta aatgacattg ttgtagacat ttgaataccc tgttgaatta
    32341 atgccataaa ggaaaaaaat acaagtcttt tatccttgca gatagataaa tattttttaa
    32401 ttgatatttt ttcaggtatt tttttagaat tatgattttt tatatttaag gctagaatcg
    32461 ttccaataac tgaagacaat acactcatga tacataaggc tgtcattcct aatttttcat
    32521 aaaataaagt actcataaca aatccaatca acattcctag attgtttacc aatattatcg
    32581 tacttgtagc tttttgttga tgttctgtag aaaaaaagtt ggtatagtga accatgtaag
    32641 aaatccaagt tgatgatgat attccagata aaatgttagc tataagaaaa ccattgcctc
    32701 caggagaaaa gactcttata agagatgcta aaccagcaaa gaaaattcca gacagtataa
    32761 atattttatg tttatttatg gaatcagcaa atactccaac aggaagcctt aataaacatt
    32821 gtgatattcc atatgcacct attattatac caatcatatt tgtagataca ttacatgcac
    32881 ttaaatatgg attttggaaa ggtatatata catattgtga aaaccagaaa aatgtaacaa
    32941 tagaaagtaa aagtaatttt tgattagata tttttttcat attatcctcc atgtggtatt
    33001 tttataatgc aaataatata ccatttagat tttttaatca agtactttct ctattaaaaa
    33061 aaatttaaat atataattat tatttacaaa aaaattatac tttatagttg aattaagaaa
    33121 acgcttatga tactataaag ttaacaaatt tgaatatata acagtagacg tgttactggt
    33181 aatgcaggca agatcgaatc aatttttata gattatctat agagtactat gggtataatc
    33241 tatttatttg gttcgatttt tttgtttaat tggccataaa acaaaaagcc tatagttgaa
    33301 aatggaggat tttttatgga ttataaaaat atagctcaag aaattctact aaatgtagga
    33361 ggaaaagaga atgtaaatga agtgactcat tgtatgacaa gacttagatt taaagtcaaa
    33421 agcgcttcta aagtgaataa ggataaatta tctaaaactg aaggagtaat aactgttgtt
    33481 gagagtatgg gtcaaataca agttgtaatt ggaaataaag taaaaaaagt atatgatgag
    33541 gtcataaaaa ttgtacctca atcaaataac ataggtaaaa agaatgaaag tcaagaaaag
    33601 caaggtataa ttaattctat attatcagca gtagctggta tatttacacc tacaatacct
    33661 gctatagcag gggttggtat gattaaaggt attttatcag ttcttgctat gtattatatg
    33721 aataaaaatg gagtagatat aaaagaaacg caaagctata ttatcttaaa tgctatggct
    33781 gattctatat tctattttat gcctattata ctaggatata ctgcggctaa agtatttaat
    33841 gcaaataaaa taatatctat ggtattagga gctacacttt gttatccaac ttttactgct
    33901 ttgatggcag gtgaagaatc tgttagattt ttaggattgg cagtaacaaa agctacgtat
    33961 acatcatctg ttattccaat aattattgct atatgggctc tttcatacgt tgaaaaagta
    34021 ttagaaaagt atatacctga ggtaattaaa ataataatgg ttccaacttt atcattggta
    34081 gtaatgttac ctgcaacatt atttttattt ggaccaatcg gaatttatat aggaaatgtc
    34141 ataaactttt cttataagta tatttatgaa ttaagcccag cattgtgtgg agcatttgta
    34201 ggtgggttat ggtgtgtgtt agttatattt ggtgctcata gagcattatt accaataggt
    34261 ataagtgatg ttgctcaaac aggaagacaa aatttattgg cgtttgcagg agctgcaaac
    34321 ttttcacaag caggggctgc tctaggagta ttctttaaaa ctaaaaatca aggactaaaa
    34381 acaatttcta tgtctgcaac tataacagct ttatttggta taactgaacc tgcaatatac
    34441 ggtgccaatc ttagattaaa aaaacctatg gtatgtgccg taatatgtgg tgcatttgga
    34501 ggtgcgataa tgggaatggg tggagcttat ggtaatgcat ttgcaaacca aggtgtttta
    34561 actatacctg tatatgctga agcaggtgca ttaggtttct taagttatct aggtggttgt
    34621 gctatagcat tttttggttc agctatttca acttatctag ttggatttga agatttggaa
    34681 gagtcttcta ataaggaaaa ttcatctata aaagtagaaa cgaaggatgg tgttattgat
    34741 ataacttcac ctgtagaggg cgaatgtata gaattgtcag aagtaaagga tgatgtattt
    34801 gcttcaaaag ctatgggaga aggtatagct gtgttaccta caaaaggagt aataacagcc
    34861 cctactgatt gtgaagtagc ttctttattt ccaacattac atgcaatagg attaaaatta
    34921 gataatggag cagaaatgtt aatacatgtt ggaatcaata cagtagagtt aaatggtaaa
    34981 tactttacaa aacatgttaa tcaaggagat ttagtgaaaa aaggtgataa acttatatcc
    35041 tttgatatag ataaaataaa aaaggctggt tatgatgtaa ctacgccagt tatagtaaac
    35101 aataca
//
LOCUS       DAEOSP010000021        34786 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.021, whole genome shotgun sequence.
ACCESSION   DAEOSP010000021 DAEOSP010000000
VERSION     DAEOSP010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34786
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.021"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            133..1044
                     /locus_tag="KRH54_000799"
     CDS             133..1044
                     /locus_tag="KRH54_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG8578471.1"
                     /translation="MYAYENIEMSLNYIEQHLSEKIETEKLAEIACLSTFYYQRLFKK
                     LVKKSVQEYIKLRRLAMVIAELNNSEERILDIALKYGFSDHANFTRAFKEVYHITPDE
                     YRKNVPMLNTFDKPELSSRYIMIDENVPLIVGNIVLEIQRKTLRTKETYFGFEKTVNI
                     AEQIPAGESTGIDVPGQLWREFHAKKLQTENAIFNNDIELGVSHLASPDKGTFLYFAG
                     GMVSSTDVCPKNMVQHTLPAGEYIVCKIEAESFEELVTTALNQANKYLFETWLPNHNL
                     VTKPFMAEKYYKGNAEINYMEVWIIPV"
     gene            1122..1556
                     /locus_tag="KRH54_000800"
     CDS             1122..1556
                     /locus_tag="KRH54_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG8578472.1"
                     /translation="MINIKDINYVPNISEISVYIENSAFDELYQHMNNEYKAVCKIEY
                     SKDVWARGWNVKFKKSGKSLCVVYPKKNYFTVLVVVGNKEKEDVENLLPRLSEYVQEI
                     YRNTREGNGQRWLMIDLKSKNNVYQDVLRLIEIRYAIKCVKP"
     gene            2024..2764
                     /locus_tag="KRH54_000801"
                     /pseudo
     CDS             2024..2764
                     /locus_tag="KRH54_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            2715..3326
                     /locus_tag="KRH54_000802"
                     /pseudo
     CDS             2715..3326
                     /locus_tag="KRH54_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            3539..4396
                     /locus_tag="KRH54_000803"
     CDS             3539..4396
                     /locus_tag="KRH54_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8578473.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERSSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGIHYLSHGKSPKIKKNYTMEDLLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPTWSHRFMGLIQWGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVASLFNADLSCLENG
                     AIQCPVILITAKGDPLFSFDYTNKVFDLIHVPQKELLALELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            4474..5055
                     /locus_tag="KRH54_000804"
     CDS             4474..5055
                     /locus_tag="KRH54_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8578474.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFALHGLKKVTVDDLVAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTIKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEDLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMIQNLLVNGIIQQVVAE"
     gene            5159..6004
                     /locus_tag="KRH54_000805"
     CDS             5159..6004
                     /locus_tag="KRH54_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8578475.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KVLTGLNTRYKGSVKVAGTEIREHTNQFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     EKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNSRLMKDIILAEKKRGKTIIITTHNMFDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIQYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            6001..6684
                     /locus_tag="KRH54_000806"
     CDS             6001..6684
                     /locus_tag="KRH54_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8578476.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALPEHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKVSEYILSKTIALIAISTV
                     IALILGVVSDSNHLLGITVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIVCFV
                     PPIVGLFVKLPYLFRFFPFTACMNLITGKSVLLSFDMVLVIATLIILYIVARHTVKHM
                     WKSLGGVKL"
     gene            6681..7379
                     /locus_tag="KRH54_000807"
     CDS             6681..7379
                     /locus_tag="KRH54_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8578477.1"
                     /translation="MKRILSSTIQVFRQIKSDPMMFAACFTPFVMGALIKLGIPFLER
                     ITDFSLQAYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLVVALVW
                     YYFLTKRLFRKLAG"
     gene            7391..8302
                     /locus_tag="KRH54_000808"
     CDS             7391..8302
                     /locus_tag="KRH54_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG8578478.1"
                     /translation="MEKGRLLQNGKVNRAIQVSKITLFINLSLSLLKFTAGYIGKSSA
                     MLSDAVHSASDVLSTIVVMVGIKISEKQPDREHPYGHERMECAASIILSVALAITGAG
                     IGYSGIKKIFSEQYNTLSTPSGIALTAAVLSIVIKEWMYWFTRNAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASVVICGCILKAALDIFKESINKMVDHS
                     CDNATETKIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELLYSA"
     gene            8354..8494
                     /locus_tag="KRH54_000809"
     CDS             8354..8494
                     /locus_tag="KRH54_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578479.1"
                     /translation="MEQYWWIIIVVVLIVVGLLKIVIMKKYNQRRNSNNSPKDSEDDK
                     MY"
     gene            8784..9410
                     /locus_tag="KRH54_000810"
     CDS             8784..9410
                     /locus_tag="KRH54_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8578480.1"
                     /translation="MSDTKENILKIALHLFAVNGYEAVSVSMIAEALGMTKGALYKHY
                     KNKQDIFDCIVERMYQIDNKRAKDYQVPESTIEVTPNAYRNTTIGSLITFTLAQYDFW
                     VTDEFASNFRKMLILEQYRNLKVNMLYQKCLVNGPISYIEDIFNEMIEKGVLQECDTK
                     QLAIEYYAPLFLLISVADAVENCWDLKTQLTTHVKQFVKKYIINEKVK"
     gene            9532..10248
                     /locus_tag="KRH54_000811"
     CDS             9532..10248
                     /locus_tag="KRH54_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG8578481.1"
                     /translation="MEYIRSKKYFTPDLQTKIMGPNPIKLQEELLQNHKIPSGSYVCD
                     LGSGQGLTSVFLAKEYGFVVFAVDLWSEPKENEEFFKKMGLSSAQIKSVKADANELKF
                     EKEFFDAVVSTDSYNYFGRDPIYLDEKLLPFVKKGGFVYITVPGMKKDCHEKLPEELL
                     LSWTPEELDYIHDITYWSNIISHCKKADLISIYEMESNSEVWSDWIKQNNEYAINDRK
                     AIEAGGGDYLNFIAIILQKK"
     gene            10412..10603
                     /locus_tag="KRH54_000812"
     CDS             10412..10603
                     /locus_tag="KRH54_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HBG8578482.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            10627..10857
                     /locus_tag="KRH54_000813"
     CDS             10627..10857
                     /locus_tag="KRH54_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578483.1"
                     /translation="MIGLKRRNMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            11124..11543
                     /locus_tag="KRH54_000814"
     CDS             11124..11543
                     /locus_tag="KRH54_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG8578484.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELKRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKKRNNVLMYYFL
                     EMSDTEIADIMNYTRGAVFKHRHRALETMKEILKEKK"
     gene            11546..11800
                     /locus_tag="KRH54_000815"
     CDS             11546..11800
                     /locus_tag="KRH54_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8578485.1"
                     /translation="MKKKYKHPSYYLISSAMDGDETAIEKLLVFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGRIREALIKMILDFEIDIDIETEEE"
     gene            12172..12375
                     /locus_tag="KRH54_000816"
     CDS             12172..12375
                     /locus_tag="KRH54_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG8578486.1"
                     /translation="MNNTDVPIWEKYTLSIEEASRYFRIGEKKLRRLAEENLDSGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            12453..13643
                     /locus_tag="KRH54_000817"
     CDS             12453..13643
                     /locus_tag="KRH54_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG8578487.1"
                     /translation="MSKVKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYAWK
                     LVPTDKTPAGKRDDISLREKEKQIKKDLDDGIDTVGGKMTVCQLYEKKNNQRKNIKRN
                     TKIGRQYLMNALENDPLGMRAIDTVKQSDAKEWAIRMNDKGYSYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFNFKLSDVLEDDTEAKVILTPEQEEKLLAFMETDNVYSKYRDEVI
                     LLLETGLRISELCGLTTHIDMLNRVININHQLLRDTEVGYYISTPKTKNGKRKLPLTE
                     RAYQALERILKNRGKAQPLVVDGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHK
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELE
                     RLAS"
     gene            13883..14704
                     /locus_tag="KRH54_000818"
     CDS             13883..14704
                     /locus_tag="KRH54_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBG8578488.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            14725..15921
                     /locus_tag="KRH54_000819"
     CDS             14725..15921
                     /locus_tag="KRH54_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBG8578489.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            16089..19487
                     /locus_tag="KRH54_000820"
     CDS             16089..19487
                     /locus_tag="KRH54_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG8578490.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            19497..21170
                     /locus_tag="KRH54_000821"
     CDS             19497..21170
                     /locus_tag="KRH54_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG8578491.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            21443..21637
                     /locus_tag="KRH54_000822"
     CDS             21443..21637
                     /locus_tag="KRH54_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBG8578492.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     misc_binding    21806..22039
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            22116..23513
                     /gene="asnS"
                     /locus_tag="KRH54_000823"
     CDS             22116..23513
                     /gene="asnS"
                     /locus_tag="KRH54_000823"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBG8578493.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(23686..24861)
                     /locus_tag="KRH54_000824"
     CDS             complement(23686..24861)
                     /locus_tag="KRH54_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG8578494.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            25280..26644
                     /locus_tag="KRH54_000825"
     CDS             25280..26644
                     /locus_tag="KRH54_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG8578495.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLVNPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            26666..27157
                     /locus_tag="KRH54_000826"
     CDS             26666..27157
                     /locus_tag="KRH54_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG8578496.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EEKAKIFLEMGILTYQKIREDSINDNSFDKLCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIII
                     DLE"
     gene            27430..28101
                     /locus_tag="KRH54_000827"
     CDS             27430..28101
                     /locus_tag="KRH54_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBG8578497.1"
                     /translation="MLDKVKGRLIVSCQALEDEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            28186..29052
                     /locus_tag="KRH54_000828"
     CDS             28186..29052
                     /locus_tag="KRH54_000828"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBG8578498.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            29085..30587
                     /locus_tag="KRH54_000829"
     CDS             29085..30587
                     /locus_tag="KRH54_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG8578499.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            30717..31169
                     /locus_tag="KRH54_000830"
     CDS             30717..31169
                     /locus_tag="KRH54_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBG8578500.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            31329..32132
                     /locus_tag="KRH54_000831"
     CDS             31329..32132
                     /locus_tag="KRH54_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578501.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            32190..33077
                     /locus_tag="KRH54_000832"
     CDS             32190..33077
                     /locus_tag="KRH54_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG8578502.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            33096..33944
                     /locus_tag="KRH54_000833"
     CDS             33096..33944
                     /locus_tag="KRH54_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG8578503.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            34007..34696
                     /locus_tag="KRH54_000834"
     CDS             34007..34696
                     /locus_tag="KRH54_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG8578504.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
BASE COUNT        12940 a         4408 c         6576 g        10862 t
ORIGIN      
        1 aagtagtcaa gctacaggtg ctgaaagaaa aagtacacat tcacaattca attctaaaca
       61 aaatttaatg aaaggaaaag gtgtatataa ttgacagata gataagtcaa ttacaatata
      121 ccaggtactg atatgtacgc atatgaaaat attgaaatgt cattaaacta tattgaacaa
      181 catttaagtg agaaaatcga aacagagaaa ctggcggaaa ttgcctgtct ttctacattt
      241 tattaccaac gcttgtttaa aaagttagtt aagaaatcag ttcaagagta tatcaaactt
      301 cgccgtttag caatggtaat agcagaattg aataattcag aggaaagaat attagacata
      361 gctttaaaat atggattttc agaccatgct aacttcacaa gagcatttaa agaagtttac
      421 catattactc ccgatgaata cagaaagaat gttcctatgt taaatacatt tgacaagcca
      481 gaactttctt cacgttatat catgattgat gaaaatgtcc cattgattgt aggaaatatt
      541 gttttggaaa ttcaaagaaa aacattaaga acaaaggaaa catattttgg ttttgaaaaa
      601 acagtaaata ttgctgaaca aattcctgct ggagaaagta caggaattga tgttccaggt
      661 cagctatggc gcgaattcca tgcaaagaaa ctgcaaaccg aaaatgccat ttttaacaat
      721 gatattgaac ttggtgtatc tcacttagca agtccagata aaggaacatt cctttacttt
      781 gcaggaggta tggtttcatc aacagatgta tgtcctaaaa atatggtaca acatacatta
      841 ccagcaggtg agtatattgt atgcaaaatt gaggcagaat cttttgaaga acttgttact
      901 actgctttaa atcaagcaaa taagtatctg tttgaaactt ggcttccaaa tcataatttg
      961 gttacaaagc cattcatggc agaaaaatat tacaaaggaa atgcagagat taattatatg
     1021 gaagtatgga taattcctgt gtaaacagta aatgctaaat aggaattatc tgacgtaata
     1081 aaaaggaaat agaaagctta taaaggaggg catagtccgt tgtgataaac ataaaagata
     1141 taaattatgt acctaatatt agtgaaataa gcgtttatat tgaaaactct gcatttgatg
     1201 agttgtatca gcatatgaac aatgaatata aagcagtttg caaaatcgaa tacagtaaag
     1261 atgtctgggc acgaggttgg aacgtaaagt ttaaaaaatc tgggaaatca ttgtgtgttg
     1321 tctatccgaa gaagaactac tttactgtat tggtggttgt aggtaataaa gagaaagaag
     1381 atgttgaaaa cttgttacct aggctttctg aatatgtaca ggaaatttat cgtaacacta
     1441 gagagggaaa tggacaaagg tggcttatga tagacttaaa atctaaaaat aatgtatatc
     1501 aagatgtatt aaggcttatt gaaattcgtt atgccataaa atgtgtaaaa ccgtaataaa
     1561 aatatttttc acatacgtaa tctcaaggta ttatcgttga gacaagtaac ctatataatt
     1621 atgggaaatg agatttttag atacttaaga aagtacttta gggggcttgc gttatggaaa
     1681 gatttaagta caattccaca caatcaaata caatctaata aagctttact aaaaattgaa
     1741 tgcattacag ctttaaaggc tcgtacaaac tatatgtttt gtgcgggctt ttttcattta
     1801 ataggaattt ggattcgtca gagagtatat tataaaaata aaacgataag ggtaccttaa
     1861 aaataaggta cctcaggctg tagacaaagt cccggactct cgtctgggct tttcttatat
     1921 ttgagttatc cacaattcaa ctttttaaga gttatcttat taagaaaaaa gtgtatgttt
     1981 tccacccgat agttttactt ctgtgatatt tgcagatgcc actcaagtag aaagtggtgg
     2041 tacggcagaa gatgtcatgc agagttcaga aagtctggga ctaccgccta attccttaga
     2101 cacggaaagc tcaatcaagc aggggtgtaa gtattttgcg tccctgcttt cttcctgcaa
     2161 aaatcaaggt atcgacgatt tgaatgtagc gatacaatct tacaactatg gcgggggtta
     2221 tgtgggctat gtggcaggaa aagggaaaaa gcacactttt aatcttgcag agagcttcgc
     2281 ccgtgagaaa tcgggtggaa agaaagtaac ctacgccaac ccgatagccg ttgcaaagaa
     2341 cgggggctgg cggtatggct atggaaatat gttttatgtg gaattagtca atcagtattt
     2401 aactgttccg caggtgtcgg gggaactggc acaaaaggta atgaatgaag cgttgaagta
     2461 tcaagggtgg aaatatgtgt ttgggggaag caatcccaac acttcctttg attgtagcgg
     2521 acttgtgcaa tggtgctatg gaaaagctgg tatctactta ccgagaacgg cacaaacaca
     2581 gtatgacgca acccagcata tccctttatc gcaggcaaaa gctggggagt tggtcttttt
     2641 ccattcaacc tataacgctg gttcgtatgt aacgcacgtc ggtatctatg tgggaaataa
     2701 tcagatgtac catgcaggca atcagcggtt atctaacaaa gaaattgcag gacttgaatg
     2761 ttgatactat tagaaccgat atactgacta gccccacagt tacagatgta attgtgtgga
     2821 atatcgaaca gtcgggaacg gacacttttt ctgctaccta cgaagtagat cagcagataa
     2881 aagagggaga acagacaaca acagtcaagg caacctatac ggtaaaagtc catgtagacg
     2941 cttacaggga tatggtaatt attcagaacc ctacccttgc accagcaatc gaaaaatcgg
     3001 actatgagcc aaagacacca gaagcagacg caagtgtaga cgctgatacc gtgaatgacg
     3061 ctaccgcatt tctggaaaca ttctttaagc tgtacccgac agccacagca aaagagcttg
     3121 cctattatgt atttggaaat gtgcttgaac ctatcggcag ggactacctt tattcagaat
     3181 tggtaaaccc agtacttaca aaggacgggg ataatatgaa agtcaaggta gctgtaaaat
     3241 tccttgataa tcagacaaag gcgacgcagg tattgcagta tgagcttatt ctacataagg
     3301 atagtaactg gaaaattatt atgtaagaca ttttgttggc aggatctatt ttcggatata
     3361 gtctgccttt tctgtagcaa atgtactgca ctgacaagct atttcattgt attttaagca
     3421 attaaatctt tcttgacaat tttatcttaa atcatatatt tatggatatt gactttctgc
     3481 ttgcaataag ttataatatg actataaaac caaattagtc acataggagg tatgtggtat
     3541 gtataaagaa gttggtattt cttcaaataa tgttccgata acactttcta tctggtatac
     3601 agaaagaagt agtcctacca ttgttttttt gccgggtact atgtcacacc ctctcatgta
     3661 tgagaatttt ctatgtggtt tgagtgaaag aggatttaat ataataggca ttcactatct
     3721 ttcacacgga aaaagcccta aaatcaaaaa aaattataca atggaagatt tgctgcataa
     3781 tgtatatgac gcaacaactt acggaataga aaatttcggt gaaaacatag ctgtcatggg
     3841 ttccagtcaa ggcgggattt tagctgcaat ggcggcaggt agggacacaa ggttaaaagc
     3901 cgtttttcca cataatatca tgctgactac attaaaagaa actatgagct taacaaaata
     3961 tcccacatgg tcgcaccgtt ttatggggct gatacagtgg ggctttcgtg taggtggaaa
     4021 attgctgcct aactacaagg ttccaggaga cgcttattta gactatgaca gagtatttta
     4081 tagtgaacag gcaaaacaag attgcttaaa tgaccctatg cttttaccct attatccttt
     4141 aagatttgtc gccagcttat ttaatgctga cttatcgtgc ctggagaatg gggctataca
     4201 atgtcctgtt attttaatta ctgccaaagg cgacccgctt tttagctttg attataccaa
     4261 caaagtattt gatttaattc atgtaccgca aaaggaatta cttgctttag agcttaatcg
     4321 tcacatgatt tttaatgagg acacggacac tacaataaac gcattggaag ctaccttgca
     4381 taaatatctt ggatagcata ttgattatat aatcagtaaa tgttatactt ttcatgacga
     4441 tataaatatt ttagtcataa ggaggttatg agtttgccac gatttacgga acaagaaaaa
     4501 gaaatcataa acagtaaact tttgatagag ggagaaaagc tgtttgcatt acacggctta
     4561 aaaaaagtaa cagttgatga tttagtagct gctgtaaaca tatcaaaagg ttctttctat
     4621 gctttttatc ccagtaaaga acatctatat gttgaaatca attttcgctt acaaaaagag
     4681 ttatttacaa gtatagaaac aaccattaaa aagaaaaagt ataagaacca tagagattta
     4741 gcaaaggacg ttatcatgct aagtttaacg ggggtgatta cttcgcctat tctttcacag
     4801 attgatttat ctttaatgga ctatctgcaa agaaaaatat cgtcagatat ttttgaaaat
     4861 catatgtaca gtgatattcg tatattggag atattggaag atttaggggt tgtatttttg
     4921 gttccgcata cggttattat aaaatccctg tattctgttt tatcctgttt ggaacaattc
     4981 aaagaggacg aagaactgaa tatgatacaa aatcttttag tgaatggtat tattcagcaa
     5041 gtagtagcag agtaacaatt ttgtttggtt atttgacagg caggtagcca aacaaatttt
     5101 ttacagttta tatgactata tatctataag cgtcataaag ttaaaggagg aatgaataat
     5161 gattgatgta aaaaaactgt attttagcta tactgacaaa ccgtttgtgg agaatgtaag
     5221 tttccatgtt gggagaggag aaatttttgg ttttttagga ccctctggtg ctggaaaatc
     5281 aactattcaa aaagttttaa ctgggcttaa tacaagatat aaaggcagcg taaaagttgc
     5341 aggtacagaa ataagggagc atacaaatca gttttatgaa aatattgggg tagactttga
     5401 attttcaacc tgctacgaga aattcacagc acgacaaaat ctcgcctatt ttgcttctct
     5461 atatgaaaag caacctcggt ctattgatga attattgcat atggtaggct tggaaaatga
     5521 cggggacaaa aaagttgctg acttttccaa aggtatgcgt tctcgcttga atttcattaa
     5581 agcgttagtc catgaccctg atatattatt ccttgatgaa cctacaagcg gtcttgaccc
     5641 tacaaatagt cgtttaatga aagatattat tcttgcagaa aagaaacgcg gaaaaacgat
     5701 tatcattact actcacaata tgtttgacgc aacagagcta tgtgaccaag tagcctttat
     5761 cgttgccgga aaagtcagtg cattggatag cccgcataat ttgattatgt caaggggagc
     5821 tgccaaaata caatatacct actatgataa aggcgagaaa accggagaat gtctgcttga
     5881 tagaaccaca gaagataagt tgttaaaaag tttaatttcc gaaaaccgtt tgctttctat
     5941 tcatagtagt gagcctacat taaatgatat ttttgttgat attacaggga ggacacttca
     6001 atgagattgg gacgtttaat ttgtggtgat atacatttcc aatggaaata tggtttctat
     6061 tttatctact ttatattaac agttttatat gtgtgcggga ttgccgcttt gccggaacat
     6121 tggaaaacgg atattgcttc tattatgatc tactctgacc cggcagcaat gggattattt
     6181 tttatgggag cgattgtcct cttagaaaaa agtcaaaagg tgttaaatgc tatggtagta
     6241 tcgcctgtaa aagtatcgga gtacatactt tcaaaaacaa tcgcgttgat tgctatttct
     6301 acggtaatcg cattgatatt aggggtagtt tccgacagca atcatttact tggtatcaca
     6361 gttggtacgg ctttgacctc agcaatcttt actatgcttg gaattattgc agctacaaaa
     6421 atttcaaact tgaaccaatt tctcattgtg attatgccga tagaaattgt ttgctttgta
     6481 ccacctattg ttggtctgtt tgtgaagtta ccatatctgt tccgcttttt tccattcaca
     6541 gcttgcatga accttataac tggaaaaagc gttttactat cgtttgatat ggtacttgta
     6601 attgctacgt tgattatcct ctacatagtt gcgcgacata cagttaagca tatgtggaaa
     6661 agtttaggag gtgtaaagct atgaaaagga tattatccag taccatacag gtatttagac
     6721 aaatcaagag tgaccctatg atgtttgcag cttgttttac gccttttgtc atgggagctt
     6781 tgattaaatt aggtattccc tttcttgaaa gaataacgga tttttctttg caagcatact
     6841 atccgatttt tgatttacta ctttctatca tggctcctgt attactctgc tttgcatttg
     6901 caatgattac attagaggaa atcgacgata aagtgtcacg gtacttttcg attacccctc
     6961 ttggtaagtc ggggtatctc tttacaaggt tgggagtacc ctcaattatt tcggcggtca
     7021 tcgcttttat tgtactgtta cttttttcat tagaaaagtt gtccattgga atgacaatat
     7081 gtttggcgct tctcggctca gtacaggcaa ttattgtttc acttatgatt atcacattat
     7141 caagcaataa attagagggt atggcagtta caaaactagc tgcacttaca ttgctcggaa
     7201 taccagcccc cttttttatt gatagttact accagttcgc ggttggcttc cttccatcat
     7261 tttgggtagc gaaagccatg cagaatgagg cggttcttta tttctctata ggattggtgg
     7321 tagctttagt ctggtactac ttccttacaa aacgtctgtt tcggaagctg gcaggataag
     7381 gaggcatatt atggaaaagg gtagacttct gcaaaacgga aaagtaaata gagctattca
     7441 ggtttccaaa atcacactat ttatcaacct aagtttatcg ttacttaaat ttactgccgg
     7501 atatatagga aagtcaagtg ccatgctgtc ggacgctgta cactcggctt ctgatgtatt
     7561 aagcacaatc gttgtgatgg ttggaattaa aatatcagaa aaacagcctg atagagaaca
     7621 tccttatggg catgaacgta tggaatgtgc tgcttccatt attctctctg ttgcattggc
     7681 cataacaggt gctggaattg gatattcagg tatcaagaaa atcttttcag aacagtacaa
     7741 tacgctttct actcctagtg gaatcgcact tacggcggca gtcttatcca ttgttataaa
     7801 agagtggatg tactggttta caagaaatgc tgcaaagcat acaaattctg acgcacttat
     7861 ggctgacgca tggcaccacc gctctgatgc actctcgtcg gttggttcct taattggcat
     7921 tttaggagcg cgtttaggct atgctattct cgaccctatt gcaagcgttg tgatttgcgg
     7981 ttgtatctta aaagctgctt tggacatttt caaagaaagc attaacaaaa tggtagacca
     8041 tagttgtgac aatgctactg aaacaaagat tagggaagtt gtgcttcagc agcaaggggt
     8101 tgatggtatt gatgaattaa aaacccgcat gtttggagcg aaaatgtatg tagatattga
     8161 aatattagct gatggtaatt tagctcttta tgacgctcat agaattgctg agggagtcca
     8221 tcaaacgatt gaaaacaact ttccacaatg taaacattgt atggtacatg ttaatacgaa
     8281 tgaacttctc tattctgcat aggacaggga agatgattta aggctattga aagggaaagg
     8341 agggaagtag gttatggaac aatactggtg gattatcatt gttgtggttt tgattgtcgt
     8401 tggtttactt aaaattgtaa ttatgaagaa gtataaccaa aggagaaatt ctaacaatag
     8461 tccaaaagat agcgaggacg acaagatgta ttaaggttta ttgaaatctg ttatgttgta
     8521 gaatgatgtt tttagatact agaaagtgct ttaagggggc taacgttatg gaaagctttg
     8581 agtacaattc tttccaatca acagtatttt gcatagagaa atgtatgtag gaaatatggt
     8641 gcaaggcata aagcaccagc gatcgagaga tcaacctatg aaccaaaggc acactacatt
     8701 ttatactact tgacaagtaa acgagcgttt actataatgg tttacagaag taaacgttcg
     8761 tttactaaag aaggaggttg taaatgtctg acacaaaaga aaatatatta aaaattgcac
     8821 tacacttatt tgcagtgaat ggttatgagg ctgtttctgt tagtatgatt gcggaagcct
     8881 tgggaatgac aaaaggagca ttatataaac attataaaaa taagcaagat atttttgact
     8941 gtatcgtaga acgtatgtat caaattgata ataagcgagc taaggattat caagtaccag
     9001 aaagcacaat agaggtaacc cctaatgcat acagaaatac aactattgga agtctaataa
     9061 catttactct tgcacaatac gatttttggg tgactgatga atttgcgtct aattttcgca
     9121 aaatgttaat actagagcaa tatagaaatt taaaagtgaa tatgctttat caaaaatgtc
     9181 ttgtaaatgg tcctatcagt tatatagaag atatatttaa tgaaatgatt gaaaaaggtg
     9241 ttcttcaaga gtgtgataca aaacagcttg ctattgaata ttatgcgcca ttatttctat
     9301 tgataagtgt agcagacgca gttgaaaatt gttgggattt aaaaactcaa ttaactacac
     9361 acgttaaaca atttgtcaag aaatatatta taaatgagaa agtaaaataa tcctttattc
     9421 tgtcctaaat ataaacctaa ctttttaatt tcagcagaga aactcaaaat gaagattgtt
     9481 ataagtcaca aatattaact attaaaatat tacaataacg gagatgagaa aatggaatat
     9541 attcgcagta aaaaatattt tacacccgac ttacagacaa aaattatggg gccgaatcct
     9601 ataaaattac aagaggaact attacaaaat cataaaatcc cgtctggtag ctatgtctgt
     9661 gaccttggaa gtggacaagg tttaacttct gtatttcttg caaaagaata tggatttgtt
     9721 gtttttgcag ttgacttatg gagtgagcca aaagaaaatg aagaattttt caaaaaaatg
     9781 ggattatctt ctgcacaaat taaatctgta aaagctgacg ccaatgaact taaatttgag
     9841 aaagagtttt ttgatgctgt agtaagtaca gattcatata attattttgg acgtgaccct
     9901 atatatttag atgaaaaatt gcttccattt gttaagaaag gtgggtttgt ttatatcaca
     9961 gttcctggca tgaagaaaga ttgccatgag aaacttccag aagagttatt gttatcatgg
    10021 acaccagaag aacttgatta tatacatgat ataacatatt ggtctaacat aattagtcat
    10081 tgtaaaaagg cagatttaat ttcaatatat gaaatggaaa gtaattcgga agtttggtcg
    10141 gattggataa agcaaaataa tgaatatgca ataaatgacc gtaaggcaat agaagctggc
    10201 ggtggagatt atctgaattt tattgctata attctacaaa aaaaataaat tctttagaac
    10261 caaacactat aatttgcgtt ctaatatgtt taccgattga catcaataat tttatttttg
    10321 agggtaatga tttgaaaata gaagaataat tccagtttgt aaaagtcaat aaatccaaag
    10381 ataaatttgt atttgaaagg agttttgaaa aatgaaaaaa tatgagtatg tagaaattaa
    10441 ctataatgcg aaaggagttg tattcctttg tactgataaa cacaaagaaa ttattgacag
    10501 ttacgcaaaa acggggtatc actacgcagg gtttgtaccc acagaaattg acgcaaaagg
    10561 ctgtatgcga aagatagatt taatttttga aaaagaagat taacttattt tagggagttg
    10621 gttctaatga taggacttaa aagacgaaat atgaattata ttatgaaagt tggtgctaaa
    10681 gctcaaggtg tttctgtatc tgaatttagg gctatgctac aagataccat agacaaaact
    10741 atggaaagta cagacccaga cgtgcaggca aattttaaac gatactttgg aaataagcgc
    10801 ccaacaccag aggaatatat ttatacgatt accaaaaaga caaaggttaa ggtttaaatg
    10861 ttgtaaaggg ggcttgccgt atgaaacgct ttatgtacaa ttcagtatat tcaagcccaa
    10921 tttaacagca tactatttac aattaaatac gctataccag tagctcgtac aaaatttata
    10981 ttttgtgcgg gcttttttaa tacccgaaaa gtttttttaa ttattttcat ttttttggaa
    11041 gcaaatcaca ccttgctgta cagatatggt gcggaaagag ggataaacac ttttcaattc
    11101 atagctggaa aggaggtgag attatgaaac cgtctgactt ccaaaaaaca attcagtgtc
    11161 agtttgaatg taagctgaaa aaagttgtca aaggtattat ctggaactac cgtaaagaat
    11221 taaaacgaag aaaaaacaag gaaatatctt ttagcgaatt accagacatt acggtagaaa
    11281 gtctggcaac atgggacgac tacgaaacaa actacacaat ttttaatgtg tgtggcatag
    11341 atattaaagt ccttgatgat gaacttgcag aagctctcaa acagctaccc gaaaaaaaac
    11401 gtaacaatgt attgatgtat tacttcttgg aaatgagtga tacagagatt gcagatatta
    11461 tgaattatac cagaggggct gtgttcaaac acagacaccg tgccctagaa actatgaaag
    11521 agatactaaa ggagaagaaa tagaaatgaa gaaaaagtac aagcacccgt cttattattt
    11581 gatttcatca gctatggacg gggatgaaac tgcgattgag aaattactgg tattctatga
    11641 cccgtacata tcaaagtgtt gtttacgtcc tttatatgat gaatacggca atgtttatat
    11701 tgttgttgat atggaattaa agggacgtat tcgagaagcc ttaatcaaaa tgattttaga
    11761 ctttgagatt gatatagata tagaaaccga agaagaataa gcagatacca gcagggcgtg
    11821 attggttaaa cctcctcttt cctgccctgc tcctgctttt atacatggaa gcctacggct
    11881 ttcgccgatt gttctttgac aactgaataa agcagacaga tacgtttgtg atacagcgag
    11941 ccacggggac tgtacgccat gacctttccg acaagaaagt gagcgaccca cgcagtgatc
    12001 cgagagcaac ttgtggaatg gtcgctttgc catgacctgc tatcacagaa taatgatacg
    12061 tccgcatggt gcggttctgc ccacaagaat gggaatagtt gagatactaa cggagctttc
    12121 cgaagccgtc tgtctgctta tgataagaca tacagaaagg agtgttgcaa catgaacaat
    12181 actgatgtgc ctatctggga aaaatatacc cttagtatag aagaagcgtc ccgatatttt
    12241 cgtatcgggg aaaagaagtt gcgtaggctt gccgaggaaa atctggactc tggctgggtt
    12301 atcgtcaatg gaaaccgtat tcagataaaa cgaaaacagt ttgaaaaaat tattgataca
    12361 ctggacgaaa tctgatgtct tagagccgta gatatggtat aatattagta gcgtatcacg
    12421 gctcattcca tgacggaaag gagctttaca ccatgtcaaa agtgaaaaga caagacagta
    12481 aaggacgcaa tttgcgtctt ggagagagcc agagaaaaga cggaagatac gtttataaat
    12541 ataccgatat attcgggaag ccacagtttg tatatgcttg gaaacttgta cccacagaca
    12601 aaacacctgc tggaaaacgt gatgatatat cgttgaggga aaaagaaaag cagataaaaa
    12661 aagaccttga cgacggtatc gacacagtgg gtggtaaaat gacagtctgc cagctttacg
    12721 aaaagaagaa caatcaaaga aagaacatca agagaaacac aaaaatagga cgacagtatc
    12781 ttatgaacgc actggaaaat gacccattgg gtatgagggc gattgatacg gtcaagcagt
    12841 ccgacgctaa agaatgggct atcagaatga atgacaaagg ttattcctac aaaacgatag
    12901 ataactataa gcgttcctta aaggcttcat tctatatggc gatacaagac gactgtatca
    12961 gaaaaaatcc tttcaatttc aagttgagtg atgttctgga agatgatacg gaagcaaagg
    13021 ttatcctaac accagagcag gaagaaaaac tccttgcctt tatggaaaca gataatgtct
    13081 acagcaagta tcgtgatgaa gttattcttc tgctggaaac gggacttcgt atctctgaat
    13141 tatgcggact gacaacacac attgatatgc ttaaccgtgt gattaacata aaccaccagc
    13201 tattgcgtga tacagaggtg ggttactata tttccacacc taagacaaaa aacgggaagc
    13261 gtaagcttcc attgacagaa agagcatatc aggcacttga acgaatttta aagaaccgag
    13321 gaaaagcaca accgcttgtt gttgacggtt acagcaactt cttgttcttg aaccgtgagg
    13381 gattgccaaa ggtggcagga aattatgaaa gtatggtgcg tgggctgatt aagaagtaca
    13441 acaagacgca taaggacaag ttgccaaaca tcacgcccca ttcattcaga catacttact
    13501 gtacgaacat ggcaaataag ggaatgaacc ctaacaccct gcaatacatc atggggcacg
    13561 caaacataac catgacatta gggtattatg ctcacggtac tttccagtct gcaaaggctg
    13621 aattagaaag gctggcttct taatatcgaa aatgtatctt actacttatt tactactttt
    13681 ggttgcattt tcatgctggt aaatgccatc ttatgcgagg tatcttccaa aaggaaaatg
    13741 ccaataaagc ccgaaaatat gggatatatc ggcatttacc aacttatgaa aagataatca
    13801 gaaatttagt aagtttttat atcaaaaata gtaataaagt ctatatacat ttagaaaata
    13861 ctgtttaaaa ggagtaggat ttatgataaa aaaagagata tctaacttta gaggaagtag
    13921 tgattatgta gtatcaccag aattgatggc atcagttaat gttgctattg ctcttgaaaa
    13981 gccacttttg attaagggcg aacctggaac aggtaaaact atgctagcac aagcaatttc
    14041 aaatgaatta aaaaaagatt tagtaatttg gaatataaaa tcaactacaa aagcacaaga
    14101 agggctttat gtatatgata ctgttcaaag actttatgat agccaatttg gaggagaagg
    14161 tgtagatgat attagcaagt acataaaata tggtaaacta ggagaggcct ttagctcaaa
    14221 tcaacaagta attcttttaa tagatgaaat agataaggcg gatttagaat ttccaaatga
    14281 cttgctttgg gaattggata aaatggagtt ttacataaat gagacaaaag aaactgtaag
    14341 agctaaacaa agaccaatag ttattataac ttcaaatgcg gaaaaagaac ttccagatgc
    14401 ttttttaaga agatgcatat ttcattatat agaatttcct gatagagaca tgatggaaga
    14461 aatagttaaa gtacattttg ataaagtaga agaacaccta ttggaacaag taatgactac
    14521 tttttattgg attagaagtt taaaggacat acaaaaaaag cctagtacat cagagttaat
    14581 agactggata caagcattaa ctttgagtgg aatgcctatt gaaaaaatag aaaaagaagt
    14641 tccttttgca ggaatactac ttaagaataa tgaggatatt gaatctatgc aaagacactt
    14701 atagttaata ggagctggat aattatgttt attgaatttt tttatttatt gaggtcaaga
    14761 ggattaaatg tatctttaaa tgaatggatg actttaattg aagcattgga taaaggactg
    14821 tgctattcaa acttttcaaa tttttactat ctttgcagga tgattttaat aaaaagtgaa
    14881 agtgattttg ataagtttga tgcagtgttt ttggagtatt ttaaaggtat agaacaccaa
    14941 gaagaaattc cagaagagat tatgaattgg cttaaaaaac cagatataga tttagaagag
    15001 tttgaaagat tagaacaaaa tccaaatata aatcttgata aattaagagc taaattggag
    15061 gaacgaataa aagaacaaga ctctcctcat aatggtggta attattggat aggcacagga
    15121 ggcacttcag agttaggaca ttcaggaaaa gggcaaactg gtatacgtat aggtggaaat
    15181 tctacttatg gtagagctgt tcttgaagtt gcaggagaaa gaaaatacag agattttaga
    15241 gatgatgaag ttttaaatat gcgtcaattt caagttgcac taaagagact tcgtcaattt
    15301 tctaccagaa tagatgctcc aaagacagag cttgatttgg ataagactat tgaagagact
    15361 tgtaataatg caggatattt aaagttattt tttgagaagc caagaaaaaa tacagtgaag
    15421 cttttattgc ttatggattc aggtggttca atgagagggt atagcacttt atgtaataca
    15481 ttatttcagt ctgtaagtaa atcaaatcat tttaaagatg taaagatata ttatttccat
    15541 aactgtattt atgatagatt atttacaaca cctgaatgtt ggttaagtaa atctattaat
    15601 actgaatgga tattaaaaaa tatagataaa aactataaag ttataattgt tggagatgct
    15661 tctatgtcgc cttctgagtt gcttcatatt ggtggtaact atcgtggtcc atataattac
    15721 acacctggta tagagtggct taaaaggttt aagagaaagt acagtaaaat tgtatggatg
    15781 aatccagaac ttagagatgg atgggattca attaattatt ggtatcaaac tcaaagaatg
    15841 atacaaagtg aatttaatat gtttccattg actgtaaaag gtcttgagaa agcattgaaa
    15901 aatttaatgg tttcaagata aagtaaatat aaatgatggt atgatttatt caaaattgta
    15961 ccatcattat ttattttttt taatatatct attataaatt gtataactga aacttaaaaa
    16021 attatatagt ctaatacatt ttagactcac acaaaacata tacattaata ataattttag
    16081 aagtcgaggt gattataata aattatgaat taattgtaaa atacaatgga gatatattga
    16141 ggttagaaga agaattaggt gtatcagtag agatacttaa ttcatcatat gcaataataa
    16201 catcaagtaa tgaagaagat gtaaatatac ttttaactta tccagaaatt gaatttatag
    16261 aaaagccttt tatattacag actcaggatg tgcaaagttt ttcaagtaca ggaataacag
    16321 gatttaaaaa tagaactggt ttaactggaa aaggaactat aattggtata attgattcgg
    16381 gaatagatta tactctacct gtgtttagag atagtgatgg aaggtctaag atattatatt
    16441 attgggacca atctattcaa ggaaatccac cagaaggttt tagagaagga acattatata
    16501 ctaatgaaga tataaataat gcaatagatg gaagtatgta tatacctatt tcaacaacta
    16561 gcttacatgg gacacatgtt gcaggtattt gtgcaactat tgcaagtgat gccagaataa
    16621 tagtggttag agttggaaat atacagaccg atattttttc gagaagtaca gagtttatga
    16681 gggcaattaa atttatttta gatagggcat tagaattaag aatgcctgtt acattaaata
    16741 taagttatgg aagtaatgaa ggttcacata gaggaacatc tttatttgaa caatatatag
    16801 atgatatgtg tttattttgg aaaaacaata tagttgtagc agctggaaat aatgcagata
    16861 aaggaggtca taaaaggatt aggcttcaaa ataatattac agaagaagtt gagtttatag
    16921 ttggggaagg agagcgtata ttaaacataa atatatggcc agattttgtt gatgatttta
    16981 gtgtacattt ggtaaaccca tcaaataatc aaacacaagc aatctctcta acttcaggtg
    17041 agataaggaa tactttgggt gaaactagaa taactggtta tttttaccct atagcaccgt
    17101 attctctcac aagaagagtt actctacaat taagttcaaa tacacagata actcctgggt
    17161 tatggaaaat tgtatttgaa ccaatagata tagtaactgg taatgtaaat atatatttgc
    17221 caacttctga aggtctgaat agaaatacta gatttctgat tcctactcaa gagcttactg
    17281 taacagttcc tggaacagca agtagagtta taacagtagg tagttttaat tcaagaacag
    17341 atatagtatc tatattttct ggtgaaggag atacacaatt aggtgttttt aaacctgatt
    17401 tattagctcc aggtgaagat atagtatctt ttttaccagg aggtacaagt ggagcattaa
    17461 ctggaactag catggctaca cctcatgtta caggggtttg ttcattgttt atggaatggg
    17521 gaattgtaaa tggaaatgat ttatttttgt attcacagaa attaagggca ttacttctaa
    17581 agggagcaag gagattaagt aatcaatctt atccaaataa ttcatcagga tttggttttt
    17641 taaatttatc agatatagat ttgtacactc tatctaatat aaatcaagat ttagaaacag
    17701 aggatattgg atatagaagt attaataagt catttaaaga tgaggaaaat agttataagt
    17761 ttatagatgg ttataatatg caaatacata atgacttaga gaatgaaatt tatataagta
    17821 aaaatgcaag tagacaatca ggaatactat caggaataga tatagtccat actcctgaat
    17881 ttgaagaaga actagctggg ttaggtatga gtcaaaggtt ttttaagata agtgattcat
    17941 taggtgtact atctataaat aatacagact ataatagcat acaacgagtt ttgcaacttc
    18001 cttcaatcat aagaactgta tcgactacaa agatgacatt acttggagaa attaatcgag
    18061 gtacttttgg aggtgtagtt gcaacagaag aaatgggcgt aaatttcttt aaaaataatc
    18121 ctaatattaa tataacagga agagggactc taatatcaat tgcagataca ggaatagact
    18181 atctacaccc agattttatt tatccagatg gcacttcaaa aatagtatat ttatgggacc
    18241 aaacaaaaga aggaactcct cctgatggtt tttatattgg gacagaatat acacgagaag
    18301 atataaatcg agctatcgca gaaaatgacc ctagtctatc tcaggatgaa gttggtcaag
    18361 gaactatgtt gagtgggata tgtgcagggc ttggaaatgt aaatagtgaa tatgcaggta
    18421 tagcagaaga ttctgagcta ataataataa aactcggcaa gatagatgat ttttataata
    18481 gtgcaatgtt gtttgctgca tctcagtatg catataaaaa agcttttgaa cttggaagac
    18541 cattagttat aaatatgtct ttaggtacca gtagcctagc aggtttaacc aataggagta
    18601 atagtgaaaa agcgttcttt actagagggt tatgtataac ggcaggagca ggaaatgagg
    18661 gcaatacaca gacacataca tctggaataa taccatatgt tggaggttct gttgaagttg
    18721 aattagagtt aaatgaagac gaggaagaat tatcattaga actttggtta aatagaccag
    18781 ataaagctga tgtaataata gtatctccca ctggtgaaga aagtaaaagt gttggaattt
    18841 caaattataa taaagtaaca ggtttatttg atttagaggg gactgaatat tctataacat
    18901 atatataccc aacaactttt tctggacagc aatttactaa tgttacttta aagaatgcaa
    18961 aaagaggtgt atggaagatt cgattagtag gagtttatat tataacagga agatataact
    19021 tataccttcc aaatagagaa cttttaaaaa gtggaactag atttagagag gtagaccctt
    19081 tttacactat aaattatcct gcgattcaag atgatttaat aaccatagga gcctacaata
    19141 ctattaatgg tagtttatgg caaagttcat caagagggcc aactattgaa gacaggttaa
    19201 aaccagatat agtagcacca ggtgtcaata taatagctgc ttatcctggg aacacgtatg
    19261 caactataac tggtacagca gcagcaagtg cacatgcagc tggtgctgct gcaatgtatt
    19321 ttcaatatac ttttgttgac ggtagatatc caaaccaagc atatgtgcaa aaaataaaaa
    19381 cttttatgca agctggtgct agaaaagatt caaacacagt atatccaaat acaaattctg
    19441 gatatgggct cttggatgtt agaggaatgt ttgatgtttt aagataggag ggatttatgg
    19501 aaaaatctta ttgtataatt tatcaaggtg atatagaatc tgccctacaa gaaaatggaa
    19561 taaataggta tatggtttta aatagtcagc ttgcagtaat atatgtacct ttagattttg
    19621 atgaaactat tttaaataat ataatacaag ttgcttggtg ggaagaatct gaaccaatga
    19681 gcagtctaat tgaaataact aataatgtaa ataatggaga aaccattaca acagcagctg
    19741 aaactgatta tatttatgaa aatccatata atgatataac agggagagga attttattag
    19801 cagttataga ttctgggata gattatttgc atcctgattt tataaatgat gatggaacta
    19861 gtaaagtttt atatttatgg gaccaagaag caaatacaaa tccacctcca gaaggtttta
    19921 tttttggaag tgaatttaca agaagtgagt taaatatagc cataaataga aatgatggga
    19981 gtttgagtca agataatata ggtactggaa ctttagtttc tggtatatta gttggaaatg
    20041 gaaggataaa ttctcagtac agaggtatta ctacagaatc agacttaata gtagttaaat
    20101 taaaatcata tacagatact tattatgctg gaaggataaa ttacagtgtt tcagattttt
    20161 tagcagctat aacgtatgtt actaacatag caagaacaga aaataaacca ctgataataa
    20221 atttgactat tggagttaag tcaagtgctg tagcaactac ttcaatacta gatactttta
    20281 atatattgag tagtgctggt gtggtagtag tgagtggtgc aggaaatcaa gggaatacag
    20341 atattcatta ttctggcaga tttagcagtg tgggagaagt tcaagatgta ataatacaag
    20401 atggagatga ctatgctctt gatataacac taaatacgaa tggtccagat aaagtgggag
    20461 cacagataat atctccttca ggtgaagtta gtcatgatat aaggtattct ccagactttt
    20521 atatatatag aggaaaattt aacttagaaa atactactta tgcaatgaga tttatttatc
    20581 catatattac ctctggaaaa gaaaatttgg agataagatt aagagatata aaacctggag
    20641 tatggatttt aagattgact tcagaactta taataaatgg agaatatgac atatacttgc
    20701 caaataaaaa tttgatagct ccagacacaa ggtttttaga ccctgattca gtagctacta
    20761 taactatgta tgctgctagt gatgatgtaa ttacagttgg tacatttaac aataagactg
    20821 atagtatgtg gattggttct tcgaaaggac caattagagg aagaggaatt aagccagata
    20881 tagtagcttc aggtgtagat ataatatcca catataaaaa tggaacatat aatacaggaa
    20941 caggaacagg agtgagcagt tctatagtca caggtgtttt agccttattg atggaatact
    21001 tagaaaaaca ggataatgta ccgagattat cattgtttac acaagtttta aaaacatatt
    21061 taatattggg tgctacaaaa cttgagatat atacatatcc aaatgtatcg cagggatatg
    21121 ggattttaaa tttaaaaaat acgattcaac agatagcaaa tactctatag ataagaacct
    21181 atgtaaaatt ataattgatt attagtttaa ttgtatcaaa tactttagat agagtagaaa
    21241 aatagcataa aaaataaata tgaaaattaa aaaacagttt tatattttga atacacaaag
    21301 taaatatttt gtatataaat aggtataaat taagtattga aaagatatga tgttaattta
    21361 aaaataatta taatttagac ttatgaattt tatttgtaaa attgctaaaa ctattacaaa
    21421 ataaataata gaggtgatag ttttggaaag taaaaaggca agagcattaa aaggtaaaga
    21481 taataaaaga cttaaaagtt cacttttagt tggaaatgag tcgacagcag catgggctga
    21541 tgttgaaaaa ttaaaacctc atagtaaagt ttcaatacct tcaattaagg gtgtcgaaga
    21601 agcaaaagac tgggttgaca atggaagtaa attataaaat tttggaaatt gaagtaataa
    21661 agaagtaatg aagataagta tttattatca ttatgtaagg tagaattatt atattttaaa
    21721 taacaatcag tatcaattat ataaatttat tgactaaaaa tttataaatt ggtataattt
    21781 tgttaattag cagaaataat aaaagataaa tactgtgaag agaagagtag atataaaatg
    21841 tagttctagc gagttgagga tggtgaaagc tcaatacgag gtttatattg aagaacatct
    21901 tggagtttct aatcgaaagc ttatttagta agtagactta gacggaaggc accgttatag
    21961 gctttaagca ttgcttaact aagaggtcat tatgacaact agggtggtac cgcgagagta
    22021 atagtctttc gtccctttat atagggatga agggcttttt ttattgaaat tatatttaaa
    22081 agaaattaat aacaattata ctaggaggat ttaaaatgca aaaggaattt ataacagtaa
    22141 aagaattgta cagagaaaaa gaacagtaca tagggaagac tgtaaaagta gctggatgga
    22201 taagaacatc tagagtatct aaaaattttg gatttataga gctaaatgat ggaagcttct
    22261 ttaaaaatat gcagatagta tttgatgaga aagtagaaaa ttttaaagag gtaggaaaat
    22321 tagcaataag ctcatctata cttgtagagg gagagttagt atctacagaa ggggctaaac
    22381 aaccagtcga aatacatgca aaaagcatag ttgtagaagg ggaatcagat agttcgtatc
    22441 cactacaaaa gaagagacat acgcttgaat atttaagaac gatagcacat ttaagaccaa
    22501 gaagtaatac tttttcagct gtatttagag taagatcaat agctgcctat gcaatacaca
    22561 aattcttcca agaaagaaac tttgtatatg cacattctcc aatcataaca ggtagtgact
    22621 gtgaaggtgc aggagagatg tttagaataa caactatgga tttaaatgat attccaaaaa
    22681 ctgaagatgg caaaatagac tatactcaag atttctttgg aaaagaggct aatttaacag
    22741 ttagtggtca attaaatgcc gagataatgg cactttcttt tagaaacgtt tatacttttg
    22801 gacctacatt tagagctgaa aactcttata caggaagaca tgcttcagaa ttttggatga
    22861 tagagccaga aatagttttt gcagaccttg aagataacat ggaacttgca gaagatatga
    22921 taaaatatgt tataagctat gtattagaaa atgctcctga agaaatggaa ttttttaata
    22981 gttttataga taaaggattg ttagaaagat taaataagat agtaaactct gagtttatta
    23041 gaataacgta tactaaagca gtggaattat taattgaatc tgggcatgaa tttgagtatc
    23101 ctgttgagtg gggatgtgac cttcaaacag agcatgaaag atatataacg gagcaaatat
    23161 ataattgtcc agtgtttgtt acagattatc caaaagatat aaaagctttc tatatgagaa
    23221 tgaatgaaga tggtaaaact gttagagcaa tggatttact agttcctgga gttggagaaa
    23281 tcgtaggagg ttctcaaaga gaagaaagag aagatgtatt gttagataga attaatgaaa
    23341 tgggattaag agaagaagat tattggtggt atttagaact tagaaagttt ggtactgcta
    23401 ctcactctgg atttggtctt ggatttgaaa gaataattat gtatatgaca ggtatgtcta
    23461 acataagaga cgttatacct ttcccaagaa ctcctaaaaa tgctgaattt taataattat
    23521 atataataaa acttatataa ctttaagtat aaacactaaa atgacttgca tatttatcta
    23581 ttttagagat atgcaagtta tttttattgt actataaata tataatttga ttacttttat
    23641 tttgcgatat aatacttata ggtttatgta atagtatgta accttttaat aaaatgcaac
    23701 acaattacgt ccttgttctt ttgcagtata tagggcctta tcacaattac ttataaactc
    23761 atttaatgtt attgtctttc caattttctt tgtataaact ccaatactaa cagttacaca
    23821 ttttaaagga acatcatcac gctcaaaatt gcaggactct ataatttttc gtaaacattc
    23881 tgatttaagt ttagcatctg cctcattaca gttaaaaata catataaaaa attcctcacc
    23941 accatatcgt atagcatgtt cattttcaga acttgtaaaa tctaaaatta ctttggcaat
    24001 attttgtaga caatagtctc ccttttgatg tccataatta tcattaaatt gtttaaaata
    24061 atctatatct atcatcattc cagttatagg ttcttgattt gcatggctga ttaatttttt
    24121 ttgtagaata tcttcaaggt atctacggtt gttcatttta gtaaggtcat ctgtcataac
    24181 tttgtcgtta agtaaattat ttatatgaga tatttcttca tattgttgac taattaaaag
    24241 tttatcatag taagtaaata cacgatttct atatagcatt gtagaaacaa aaatggcaag
    24301 ggatgttata aaaaatgtgt tgacaatatt actaaactca ttgcctggta tagtttgaat
    24361 cccaggtaag aataaattaa gaaacaaaaa gcatcctcca aaaactatta ctgattctcc
    24421 aaattttaat actgagaaag ctgcagctgc taacataata taactaaata cacttaggtc
    24481 atttccacct aactggtcaa gtagagtaat tacacaaccc cataggcaga aaaaagtcac
    24541 ataaattatc cataaattta tataaaacgt acagcaatcc ttttttttat tccaaaggta
    24601 agaaaataca aacataaaga cagaagatac acaaaatagt gttacataca tataaaaata
    24661 ccattggcgt cttggtgtga caaaagggcc tccttctcta gctgagattg atatcatcat
    24721 aattatttga gatattacta ttattatgca aatgatttta cccgtatgat aaagataatt
    24781 gcagagttct ttctcaacat tgtctttgtg ttttttgtct agaggaaatc caaaaaattt
    24841 ttttattttt tctaagttca tataagcctc cattaaaaat aaagcataaa aataagtatt
    24901 cctttctatc attagatatt tggaatactt cgtttatgat ttaattatat caaaatatat
    24961 tgtgtgtaac aatctaaaat tgtgatagat aaaatttatt agataatgtc gagtaacttt
    25021 attgattaat actttattat aattaagatt tacagatagt ttactactga aaataaagaa
    25081 tagaataaat ttaaattaag cataataaaa tataaataat aaagttcgca ttaacaaact
    25141 atttaaatat caagaactgt taaaaaacaa cagcaataaa caaataaaat agataaatat
    25201 atccaattaa ggtataatta agttttatat gatatagtta tatttgataa tttacaatag
    25261 taaaacttgg agggaaaaaa tgggtgaaaa gaaaataaat aatatgaatt attcacataa
    25321 taattattcc aaaaagacag gtgaaccaac tagaaaaata aaaaatagcc atatcactct
    25381 taaaagtaaa gaagaaaaaa tttgttctag ttgcaataaa gaaaataata ctaaggataa
    25441 ttactgtaaa ttttgtggaa atgaacttta tgaaatagca tcattaagac cacttgaaac
    25501 aaaactagac ttgaaatcta aaataaaaga gctttcatat catgcaaata aaagaggtgt
    25561 atttttaaca acatttacaa ccatatttat attgtttatt attgcattga tatttaaagc
    25621 tattataact attcaattta atgatattag ttatttagta aatccagcac atataatcct
    25681 agctctcaac ttgggtcaaa ttagtgtatc tatgtctacc atgatgggtt ctgggtttat
    25741 aaatgccaat ataggtttgt tgatattact tataatccct attttggtat tattaatatc
    25801 aaacctaatt tttatgagaa aaagatgtag agattcaaaa actgtattag caaattctct
    25861 aggagttgga attttttatg gattagtttt agctatttta tctattttta ctaatgtaaa
    25921 aactagttca catagtatgt tagaatatgg atatgcatta gaatatagtt atgaattttt
    25981 tagtgttctt ttaaatggat tcgtattagg gtttatatgt acatacataa ctacttataa
    26041 aaaaagttat gaaaaagaaa atatgtattt atctcttttt tcaaattcta taagaacatt
    26101 catattaggt tatgttttag tattagtaat actattggta ctaactatat ctgatagtag
    26161 ttacttaaat gaactagata tgagtagtta ttcaaatgga ttaaatttat ttactatact
    26221 cccacaaata gctagttata tgtgggcttt tgcaaatgga atttcagtaa ccataataaa
    26281 ctcaacagta tcaatgttta ctttaagttc ttcatcttta tttggagata ctaaattaat
    26341 gttttatgca atgggagcat tgtctatgtt aatattatta ttaaatggat acaagctaag
    26401 atttaagtat aatacagata gtataagacc tataatagta tttagtatat attatgcatt
    26461 tttgatggga atattggctt tatttagtac atttatacta gatagtaata ttaatttctt
    26521 taatacaact aattatggaa caactttaat aatgcaattt aaagtcttac aagctattgt
    26581 gatttcattt gtatatagtt ttgtgatatc tttaatagga tataagctaa attcagctga
    26641 ttaaatatga ttggagggaa tttctgtgga aggaaatact aataataaag tagatgataa
    26701 attttctaag gttaagaaaa agtttggtag tggaaaattt aaagttgaat tatataaaga
    26761 aaaatcaaaa ttaaatagag agattgatga tattcaagaa gagaaagcaa agatattctt
    26821 ggaaatggga atactgacat atcaaaaaat tagagaagat tctattaatg ataatagttt
    26881 tgataaatta tgtgatgagt tactagaact tgataagtta atttatgaaa aaaatatgga
    26941 aattaatgaa atggaaatgg cagaaagtaa tgtaacttgt gagtgtggtt atgttggaaa
    27001 tgtaaatgat aaatattgtg ctgaatgtgg taaaaaattt gaattggaaa atgaatgtga
    27061 tgattttata atttgtggtt attgtgaaag tgagatagat acagaggctg aattttgtcc
    27121 atgctgtgga agaaaaatta taatagattt agaataatat tgggaaacct atgtatagta
    27181 tacatgggtt tttttattga aattatttaa atggacttat aacagtagtt atataaaatg
    27241 aaaataaaca ccgaaaaaat gaaataaaat ttcttttaaa tgtaaaaaac aagctaatat
    27301 ataaaataaa tcagtaaaaa tatagatgtg agaaaaaaat atgaaaaaat attgcaaaga
    27361 aaaagttttc atgttattat taatacataa atcaagaaaa taaatttcag aaacgaggta
    27421 atttaaaaaa tgctagataa ggtaaaggga agattaatag tatcatgcca agctttagaa
    27481 gatgaacctc ttcatagtcc ttttataatg gggagaatgg ctaaggctgc tatggaaggt
    27541 ggagctgttg gaattagagc tcaaggagtt gaagatatta ttgaaataaa aaaagttact
    27601 ggacttcctg ttataggtat tataaagaga aactatgaag attcagatat atatataacg
    27661 ccaactaaaa aagaagttga tgaactttta acaacagggt gtgaaatgat agcattagat
    27721 gctaccaata gagttagacc aaataatgaa gacttaaaag aacttataaa gtatataaaa
    27781 gaaaacggtg ttctagtaat ggcagatatt tcaaattatg atgaggctat caaagcacaa
    27841 gagtacggtg tagattgcgt ttcaactaca ctatctggat atactccata tactaaaaca
    27901 ttagaaggtc cagattttgt attaatggaa agattagtta aagatttaga aatacctgta
    27961 atagctgaag gaaaggtaaa tactcctcaa gatttaaaaa aggtctttga gttgggtgtt
    28021 cattcatcag tagtaggttc agcaataaca agaccacaat taataacaga aaaatttgtt
    28081 aaagcaattg aaattaatta aaaattaatt tataaattaa aaaattatta tatttaacta
    28141 tatttgaaaa ttaaaattgg aggtaaagat atgagaacaa cagatatgaa gggaatttat
    28201 tcagcattat tagtttcatt tgataaggaa ggaaatataa atgaaaaagg gttaagacaa
    28261 ataattagac acaatataga tgtttgtaaa gtagatggtc tttatgtagg aggaagtaca
    28321 ggggaaaact tcatgctttc aactgatgaa aagaaaagaa tatttgaaat agctaaagat
    28381 gaagtgaaag aagaaataaa attaatagct caagttggtt cagttaattt aaaagaagct
    28441 gtagaacttg ctaaatttac aactgattta ggatatgatg caataagtgc tgttacacct
    28501 ttttactata aatttgattt tgaagaaata aaacactatt ataatacaat aataaattct
    28561 gtagataata gattaataat atactctata ccatttttaa ctggtgttga tatgagttta
    28621 gaccagtttg gagaattatt tgaaaatgaa aaaataatag gtgttaagtt tacagcagca
    28681 gatttctact tattagagag aatgagaaaa acattcccaa ataaattaat atttgcagga
    28741 tttgatgaaa tgatgttacc agctacagtt ttaggtgtag atggggcaat tggttctaca
    28801 ttcaatgtaa acggtgtcag agctagacaa atattcgaat taactaagaa tgaaaaaata
    28861 tcagaagcac ttgaagtaca acatgtgact aatgatttaa taacagatat attaggaaat
    28921 ggcttatatc aaacaattaa attactactt gaagaacaag gtgttgaggc tggatactgt
    28981 agacaaccta tgaaagaagc aactgatgaa atgaaatcaa gagcaaaaga aatatacaga
    29041 aagtattttt aataaaatac ttttagtatg ggaggattag tcgaatggta ggatttactt
    29101 ggatagattt tgcaatacta gtggtttact tattggcagt tttatttgca ggattgttat
    29161 tttcaaaaaa ggaaatgaag ggaaaagagt tcttcaaagg tgatggaact attccgtggt
    29221 gggttacatc agtatcaata tttgccacat tattaagtcc aatatcattt ttatcattag
    29281 caggaaattc ttatggtggt acttggattt tgtggtttgc acaattaggt atgtttatag
    29341 caataccttt gacaataaaa tttttcttac ctctatatag taggttagaa atagatacag
    29401 catatgaata cttagaaatg agatacgaaa gtaaaggtct tagagtactt ggagctttga
    29461 tgtttataat atatcaaata ggacgtatgt ctattataat gtatcttcca tcaatagttc
    29521 tggctggtct tacaggaata cctgtaaatg tactgataat agttatgggt gtaatagcaa
    29581 taatttactc atatacaggt ggattaaagg ctgtcttgtg gaccgatttt atacaaggaa
    29641 tggtattaat agtaggtgtt acagggacat tgttctattt aatagcaagt ataaatggtg
    29701 gttttggaac agtaatggat actcttacaa ctggacacaa gttcttagca agtaatgaag
    29761 ttatgtttga taaaaatata ctatctacaa gtgcatttat aatatttgtt ggagctggtc
    29821 ttaatacttt ttcatcatat gtatcgagtc aagatatagt tcaaaggttt actacaacaa
    29881 ctgatataaa gcaacttaat aaaatgacat atggaaatgg tgttttatca atgggacttg
    29941 caacagtatt ttatctaatt ggaacttgtt tatttatata ctatactcaa aacccagatt
    30001 tagctcaaac agttcaacaa gaccaagtat ttgcatcata tatagcttat gaattaccag
    30061 tgggtataac aggaatacta cttgcagcga tatatgcagc ttctcaatca actttatcga
    30121 cagggcttaa ttcagttgct acaagttgga ctttagatat acaaactatc ataacaaaag
    30181 atataagtat ggaaaagcaa actaagatag cacaatatat atcattaggt gtaggtatac
    30241 tttctatagc tgttgcaata gtattagcaa atggagaaat aaagtcagca tatgaatggt
    30301 ttaatagttt tatggggcta gtactaggtg ttttagcagg aatatttgta cttggtgcat
    30361 tttgtaaaaa agcaactaaa atgggagcat atgttggatt catagtatca gcaattttag
    30421 ttgtttattt aaaataccgt atgccagaag taacttcttg ggcatattct ctaataacaa
    30481 tcacgacttc tgtagtggta ggtacaatag ttagtgcaat agaagctaaa gttaaagcaa
    30541 aagtatcttt accaaaagct gagactactg tttattatga aaaataatat ttaaatgaga
    30601 aattgtctta tagagtgatt agataaataa tttagttact ctataagatt gtataatata
    30661 ctttgtatac aaaagtataa aaatagactt tataattata tagggagata gtgcttatga
    30721 tatttggaaa tataaatcat agtaagacat attcaacatt gcatgaagat ttacttagat
    30781 gttttgaata tgctaaagat aattcattaa tagattatga aaaaggtact tatacaatag
    30841 atggtgatga tatttttgtg aatattgtag agtataaaac ttgtgaaaaa gaagatagat
    30901 tctgggaagc tcataagaag tatatagatg ttcatttgat gcttgatgga tgtgaacgaa
    30961 tagatataaa ttttatagaa aatttagaac aaaaaatata tgaagaaaag ggcgatttct
    31021 tgcctcttga tggtaaaaat aatggataca tggaacttag aaaaggtgat tttttagttt
    31081 gttatccaga agatgctcat atgacaggaa taaaagtttt agaaaaagaa aatatcaaaa
    31141 aagctatttt taaggttttg gttagataat gataatttat ataaccacta gggctgaaaa
    31201 tagtgattta actttcagcg attagtgaga cttcgtaaat tttagttaga ttactatttt
    31261 cagccttagt tttttatgag aaaattgaaa ataattaaaa gcttaaaatt ttgtaaagga
    31321 gttaacttat ggaaaatata gatgtattct tattaatagg acaaagcaat gctagaggat
    31381 tgggaaatcc aaaagagagc atcattccaa atgaaaattg ttttgaatat ttgagtactg
    31441 atgaaattat aaatatgaga tgtgagctag agacttcaga aggtgatggt actatagcac
    31501 ctgcattttc aaatgaatgg aataagctta ctggaaataa agtttgtttt attcacaatg
    31561 caaaagatgg ttcaagaata aaaaactgga atcatgataa taactggttt ctaaatgaca
    31621 ctatagaaaa atttaataca ggctgtacta ccttaagtaa acattataat atagaaaata
    31681 aatacgttat ttggattcaa ggtgaaagtg atgcaaaata tggtagtgat gtattgtact
    31741 ataaagaaag tctaaaaaaa attgcgtata ggcttaaaga agaatgtatg attgataaaa
    31801 tgtttgtaag tttaactggt tattggcttg gagaagatga atattttatt agaacaagac
    31861 gaattgcagc agcacaggag tcggcttgta gtgaatgtga tatattatgt gtaggaagca
    31921 aaattgctat gcagtttcat gacagaggat taactattga tgatgttcat tacactcaag
    31981 aaggtcttaa tatgttagga gaggacttat gtaaaaatat ttataagtat catataacca
    32041 aagaaaaaag tgtattaagt gatactatag acttatcaga agcaaggaaa tatatctgtg
    32101 aattggaaaa gataagtaaa agatttgtgt aatataaaat tttaaataga gttgttaatt
    32161 tataaaaata atttgtatgg agatatgtta tgaaaaatta cgtttgtata gatgttggag
    32221 gtacttcaat aaaatatggt tttattagag aagatggatt tataattgat aaatcaagtc
    32281 ttgatactga agcaaaggaa aaaggtggag aaggaatatt agccaaaata aaagatatag
    32341 taaaaaaata tatagaggaa aatgaaataa gtggtatatg tatatcaact gcaggtatgg
    32401 ttgacccagt tgaaggtaaa atactatttg cattagaaga attaattcca aactataagg
    32461 gcatgcaatt aaaaaaagaa gtagaaaaag agtttaatat aaaatgtgaa gttgaaaatg
    32521 atgttaattg tgctggactt ggtgaaatgt ggcttggagc aggtagaggt gcaacatctt
    32581 ctatatgttt aacaataggt actggaattg gtggatgtat tataataaat aataaactga
    32641 taaatgggtt tagtaatagt gctggtgaag taggatatat gaatgtaaat ggaagttctt
    32701 ttcaagaatt ggcatctact agcagtctaa ttaaaaaagt agcaaaaata aaaaatttga
    32761 atgaaaatga cttaaatgga aaaataattt ttgatttagc aaagaataat gatgaagatt
    32821 gtttaaaaga actcgataat atgataaaat cattagcagt tggaatcgca aatatatgtt
    32881 atgtaataaa tccagaagtg gtaattttag gtggtgggat tatggcacaa gaaaagttcc
    32941 taaaaccaaa aatagacaaa gcattaaagg aggttcttat agagagagtt tacaaaaaca
    33001 ctaatataga atttgcaaag agacaaaatg atgcaggaat gcttggagca ttatataatt
    33061 ttttaaacaa aatataaaga atagaagggt ttttaatgag tattttagaa caattggaat
    33121 caccaacttt taaagtcaca aaatcagata aattattaat agcttatata aaagaaaata
    33181 tagatgatgt attttataaa ccaatatcac aaatagctaa agagagcaat attggtgaag
    33241 caactataac taggtttgta aaaaaaatga attttaatgg attacaggat tttaaagtta
    33301 ctttagcaca agaaatatca actatcaata aaaaaaatat tataaataaa aatatacaaa
    33361 atgatgagcc agctttggat acagcaaaaa aacttttaag ttcaaatgta accactttag
    33421 agaacacagt agagataatc aatagtaagg atgtacatga ctgcgcaagg cttataataa
    33481 atgcaaagaa agtttacttc ataggtattg gatattctgg tataatagca caggattcaa
    33541 actataaatt tatgagaata ggtcttaatt gtgtaagttt tgacagtagt catacaatga
    33601 taatgatgtc ttccataatg gaagaaggag atttaataat tgcaatttca cattctggtg
    33661 aaacagaaga gattataaaa actgtaaagc ttgcaagagc caataatgct aaaattatat
    33721 caattactga aaataaaaat tctgaattaa aggatatttc agatgtacac ttatcttatg
    33781 tatcgggaga aacagtactg gaaaccggtt caatatcttc taaattagca caatttttca
    33841 ttattgattt agtatataca caggtagtaa aagaactttc taatgaagct attgaaagga
    33901 aaattaaaac tacaaatgct ataaaattgt ttaaaaatga ataaaataag aaatttgtaa
    33961 tgttttatag agctctctta aaaatttagg aatggagaga tattacatgt ctaaattaaa
    34021 agattataat attgataaag taataatttt taataattta agtactaaaa cgaagaatca
    34081 acttaaagaa aaagttaaac taaaaagatt acaaaaaaaa gaaattttat tttatgaaaa
    34141 agatagctta gacagtgttt atatactttt agaaggaaag gtatctattt ttaagataag
    34201 tgaaaatgga gagagaaaag taattttcat attaaatagt ggggaagtca taaatgatat
    34261 tacacttgac gcttctaaaa actctactgt tggctgtgaa gcatttgaaa attcaattgt
    34321 agcttattat agtattgaag aatttttaga aattatgcaa aatgattttc aacttacaaa
    34381 ggatattatt tcttatatgg aaagaagaat aagacgttta tatagacagt tgaaaaattc
    34441 tatatccata aaagtagata aaaaacttgc agcaaaatta tataggttaa gtagagaatt
    34501 tggagtttgc tgtggagaat ggactttact aaatgtaaat attacagtta catatttagc
    34561 tgatatgctt ggctgtaaaa gggagactgt ttccagaatg ataaaggtat tgcaaaaaga
    34621 agaacttatt aaaattgaca ataaaggttt ttatgttaaa gaaactgaat tatcaaggta
    34681 ttttaaaaat aattaattat gattaagtaa gagtattatt agaattttaa ttaaataaaa
    34741 atattattaa gagaatctat ttatagattc ttttttattg tctaat
//
LOCUS       DAEOSP010000022        34499 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.022, whole genome shotgun sequence.
ACCESSION   DAEOSP010000022 DAEOSP010000000
VERSION     DAEOSP010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34499
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.022"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            51..929
                     /gene="purR"
                     /locus_tag="KRH54_000835"
     CDS             51..929
                     /gene="purR"
                     /locus_tag="KRH54_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBG8578505.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            1085..1363
                     /gene="spoVG"
                     /locus_tag="KRH54_000836"
     CDS             1085..1363
                     /gene="spoVG"
                     /locus_tag="KRH54_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBG8578506.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            1576..2955
                     /gene="glmU"
                     /locus_tag="KRH54_000837"
     CDS             1576..2955
                     /gene="glmU"
                     /locus_tag="KRH54_000837"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBG8578507.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            3050..4000
                     /locus_tag="KRH54_000838"
     CDS             3050..4000
                     /locus_tag="KRH54_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBG8578508.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      4838..4923
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            5085..5606
                     /locus_tag="KRH54_000839"
     CDS             5085..5606
                     /locus_tag="KRH54_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG8578509.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNTEGVTLTEAQSAKLLSDIGENKIYTSTTTNSLGNPLTSNTKIDNNVLY
                     IVLIDNTVMDTTK"
     gene            5757..7433
                     /locus_tag="KRH54_000840"
     CDS             5757..7433
                     /locus_tag="KRH54_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG8578510.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            7445..8653
                     /locus_tag="KRH54_000841"
     CDS             7445..8653
                     /locus_tag="KRH54_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG8578511.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSIKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            8668..10380
                     /locus_tag="KRH54_000842"
     CDS             8668..10380
                     /locus_tag="KRH54_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBG8578512.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            10373..11281
                     /gene="pilO"
                     /locus_tag="KRH54_000843"
     CDS             10373..11281
                     /gene="pilO"
                     /locus_tag="KRH54_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBG8578513.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEIIKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            11308..11874
                     /locus_tag="KRH54_000844"
     CDS             11308..11874
                     /locus_tag="KRH54_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG8578514.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            11911..12438
                     /locus_tag="KRH54_000845"
     CDS             11911..12438
                     /locus_tag="KRH54_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG8578515.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            12459..13997
                     /locus_tag="KRH54_000846"
     CDS             12459..13997
                     /locus_tag="KRH54_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578516.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKKFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYSWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKNGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            14093..15175
                     /locus_tag="KRH54_000847"
     CDS             14093..15175
                     /locus_tag="KRH54_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBG8578517.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            15156..15938
                     /locus_tag="KRH54_000848"
     CDS             15156..15938
                     /locus_tag="KRH54_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG8578518.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKKLLELYFSMVI"
     gene            16040..16690
                     /locus_tag="KRH54_000849"
     CDS             16040..16690
                     /locus_tag="KRH54_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBG8578519.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     AQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKVFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            16704..17264
                     /locus_tag="KRH54_000850"
     CDS             16704..17264
                     /locus_tag="KRH54_000850"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBG8578520.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITNCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            17281..20667
                     /gene="mfd"
                     /locus_tag="KRH54_000851"
     CDS             17281..20667
                     /gene="mfd"
                     /locus_tag="KRH54_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBG8578521.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            20698..21693
                     /locus_tag="KRH54_000852"
     CDS             20698..21693
                     /locus_tag="KRH54_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG8578522.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            21817..22356
                     /gene="spoVT"
                     /locus_tag="KRH54_000853"
     CDS             21817..22356
                     /gene="spoVT"
                     /locus_tag="KRH54_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBG8578523.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            22476..24089
                     /locus_tag="KRH54_000854"
     CDS             22476..24089
                     /locus_tag="KRH54_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG8578524.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            24196..25644
                     /gene="mazG"
                     /locus_tag="KRH54_000855"
     CDS             24196..25644
                     /gene="mazG"
                     /locus_tag="KRH54_000855"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBG8578525.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            25733..26011
                     /locus_tag="KRH54_000856"
     CDS             25733..26011
                     /locus_tag="KRH54_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBG8578526.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            26083..26325
                     /locus_tag="KRH54_000857"
     CDS             26083..26325
                     /locus_tag="KRH54_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBG8578527.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            26387..26641
                     /locus_tag="KRH54_000858"
     CDS             26387..26641
                     /locus_tag="KRH54_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBG8578528.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            26642..27175
                     /locus_tag="KRH54_000859"
     CDS             26642..27175
                     /locus_tag="KRH54_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578529.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            27181..27465
                     /locus_tag="KRH54_000860"
     CDS             27181..27465
                     /locus_tag="KRH54_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBG8578530.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            27578..28498
                     /locus_tag="KRH54_000861"
     CDS             27578..28498
                     /locus_tag="KRH54_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBG8578531.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDLIEDEEFEAMSSFIYGEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            28588..30960
                     /gene="spoIIE"
                     /locus_tag="KRH54_000862"
     CDS             28588..30960
                     /gene="spoIIE"
                     /locus_tag="KRH54_000862"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBG8578532.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVESIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            31311..33203
                     /gene="pepF"
                     /locus_tag="KRH54_000863"
     CDS             31311..33203
                     /gene="pepF"
                     /locus_tag="KRH54_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG8578533.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            33317..34282
                     /gene="galU"
                     /locus_tag="KRH54_000864"
     CDS             33317..34282
                     /gene="galU"
                     /locus_tag="KRH54_000864"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG8578534.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
BASE COUNT        14077 a         3579 c         6296 g        10547 t
ORIGIN      
        1 gcattagtta gtttaacgtt atataaaggt acttgtagga ggaaataata atgaaattta
       61 aaagaacaga gagaattgga gcaatagtta aaatactgtc tgataatcca aataaaatat
      121 atactttaag ttattttaca aatcaattta atgcagcaaa atcaacaata agtgaagatt
      181 tattagttgt aaaaaatgta tttgagaagc tacatttagg taaagttata actatttcag
      241 gagcagcagg aggagtaaaa tatataccta aaacttctat agcagaaaat gcagagttct
      301 taatggaatt atgcgagaga atatgtgaca agtctagaat actatctggt ggttttttat
      361 acttgattga cctaatttac gaccctacaa tagctgctaa gataggaaag atattcgcat
      421 caaatattga gtatgtagat gcagattatg ttgtgacaat ggaaacaaaa ggtataccta
      481 tggcacttat gactgcaaaa gctatgaatt tacctctagt tataataaga aaagatataa
      541 aggtatcaga aggtcctaca cttagcatga catatgtgag tggaaacagt tctaaagttg
      601 aaagtatgag tttgccaaga aaagctctta aaccagatag taaagttata attatagatg
      661 atttcatgag gggtggaggt actataaaag gaatggtaga ccttatgaat gaatttggtg
      721 cagaggttat aggtactgga gtattcatat ctacaacgaa tccttcagaa aaaatggtaa
      781 aagattatat ttcattgatt caattggatg ttaatggaga taaaatagta gtagaaccaa
      841 acttaaagac ttttaaagat gaatatagaa atgaagattt agacgaagaa gaagattcag
      901 aatttgaatt tgagatagat gaagattaat aaaaaaaata tttagaaaaa tatgtaaaaa
      961 taaaaagagg atatccctag ttgttcatag aattatttaa aaaacagttt atattaattt
     1021 attccagcga taataatttt aatataaatt aatttctgtc aaaatcaaag ggggatattt
     1081 tggtatgaag ataactgacg taagagtaag aaaattaaca gaagaaggta agatgaaatg
     1141 tatagtttca ataactttcg ataatttgtt tgtagtgcat gatataaaag ttatagaagg
     1201 ccataatggg ctatttatag caatgcctag cagaaaagta ggcgaaggaa atttcagaga
     1261 tatagctcat ccaataaatg cagaaatgag acaagtttta gaagatgcag ttttacaagc
     1321 gtatcatgaa gcattagttc aatgggaagt agctgctgaa taataataaa tatataaaat
     1381 ggagagagcc cgaacattta ggctcttttt aaatgtagaa tttctattaa aatatgtact
     1441 ttttagtaaa aaagtgctat attattagtg ataatgtata tgttgtatac aaattgttag
     1501 ataaatgtat tataatgtat attgattaag agaataatac gaataaaatt aaattagatt
     1561 gaatggagtg tatcaatgaa ctttaaagct ataatccttg ctgctggcaa aggaacaaga
     1621 atgaagtcta aatacccaaa agttatacat aaagtatgtg gaaaagaaat ggttaaccat
     1681 attatagatg tttcaaaaaa atcaggggta aaagatactg tcgtgatttt aggacatgaa
     1741 gctgatgtag ttaaagaaaa gcttgcagaa gaaattataa tagccatgca aacagaacaa
     1801 cttgggacag gacatgctgt aaagatggct aaagaatata ttaatgatga agatacaata
     1861 gttgtacttt gtggagatac tccacttata aaagaagaga cactaaagag actatttgaa
     1921 tatcatatag aaaataaata tcatgctaca gttcttacaa caagagttgg aaatccaaca
     1981 ggttatggta gaataataag agataagaag ggtgaccttc ttaaaatagt ggaacaaaaa
     2041 gatgcaaatt cagaagaaaa gatgataagt gagataaact ctggaattta ttgttttaat
     2101 ggaaagagtc ttagagaagc actagatttg ctaaataata acaattcaca aggtgaatat
     2161 tatttaactg atactgctaa aataatgaga gataaaggat taaaagttgg tgcatttgca
     2221 ggttcaacta ttgaagaact tatgggcgtt aattcaagag tagaattatc aaaggcagaa
     2281 gaaattatga gacgaagaat aaatgaatct catatggtaa atggagtaac tataatagac
     2341 actaactcta cctatataga atctgatgtt atgataggca atgatactat aatatatcca
     2401 ggtgttatgt tacaaggaaa aacaaggata ggttcagatt gtataattgg aatgaattca
     2461 tctataacaa attcagaaat aggtgatggt acagaaataa aaaattcaac tataattgat
     2521 agtaaggttg gagaaaattc tactgttggg ccatatgcat acttaagacc aaagagtgac
     2581 ttaggaaata atgtaaaaat aggcgatttt gtagaagtaa aaaatgctat tatagaagat
     2641 ggctcaaaag catctcacct ttcttatata ggagatgcac atgtcggtaa aaatgtaaat
     2701 attggatgtg gagtagtatt tgtaaactat gatggtaaaa ataaatttaa atcaatagta
     2761 aaagacaatg cgtttatagg ttcaaattct aatttagttg caccagtagt tgtagaagaa
     2821 aaaggatata tagcaacagg ttcaacaata acacatgatg tcccagatgg tgctttggca
     2881 atagcaagag aaagacaagt tatcaaagaa ggatgggtag aaaaaaagaa tcaaaaagac
     2941 gaccaaagta agtaattatg tagtttatta ttttggctaa aaaatttagc tcgaaattat
     3001 tataaattat attcattagt attttaaaat tttcaggagg gttacataaa tgaatactag
     3061 cggaagcgag attaaaatta ttgcaggtaa ttcatcgaaa gagttagcac aaaaaatagc
     3121 tgactatata ggtgtatcag tactagattg tgaagtaggt acatttagtg atggtgaaat
     3181 atgtgtaaac atgaatgaga cagtaagagg ttgtgatgtg tttgtagtgc aatctactaa
     3241 cagcccagta aatgataact taatggagtt attaatctta attgatgcgt taaagagagc
     3301 atcagcaggt agaataacag cagttattcc ttactatgga tatgcaagac aagacagaaa
     3361 ggctaaggca agagatccaa tcactgctaa attagttgca aatttaataa ctgctgctgg
     3421 agcagataga gtattaacaa tggatttaca tgcagctcaa atacaaggat attttgatat
     3481 accattagac cacttactag gtggaacaat attagcaaat tattttaatg aaaagaaaat
     3541 agaagattta gtagtagtat ctcctgactt aggaagtgtt actagatcaa gaaaatttgc
     3601 taatacttta aatggagaag ttccaatagc tataatagat aaaagaagac cgaaagctaa
     3661 tgtatgtgaa gttatgaact taataggtga tgttaaaggt aaaaatgtta tactattaga
     3721 cgatatgata gatacagcag gaacaatagt taatgctgca aatgcactta aagagtttgg
     3781 agcaaaagat gtttatgctt gttgtactca tggtgtgtta tcaggtccag ctattgaaag
     3841 aattgctaat tcagaaataa gtgagttaat agttcttgat actatacaac ttcctgaaga
     3901 gaaaagaata gataagataa agataaaaac agtagctcct ttatttggag atgctataag
     3961 aatgatattc tctaatgaat cagtaagtaa attattctaa tttattgtat ttatgagagt
     4021 tgtttaaaag gatataaacc ttttgagcaa ctcttttttg attttatact tatggaatgt
     4081 tatttttttc ttgttagaaa ggtatatatt attgtaaaaa gagattatag tttaaaatta
     4141 tgtatattta agtaaaataa acctaataaa gggtatatta ttcaaaatat tatgaatata
     4201 aaaaatatta caaaaaagta ttgaaaaaat tcagattatg tagtattatg taaatataaa
     4261 taaagaaaga ggtgtttcca atgggaaggt tagaagatga acccctaata attaaatgat
     4321 tcagtcacaa tctaaagtga atataaaaat ctattgaaag tttatttata agaattgaaa
     4381 atagtatcta ttctatcttt agtgtttgtg agagggttct ggattatata agattactgt
     4441 aaagagtaat tttgcacaat aagcatatct gtaaatgaag agtttcgttt atataatgat
     4501 tgtggctgaa tcataactat aatagataga atgtttataa tagatgattt tataaaatac
     4561 tatattgtaa tagcttttag ctttcaaata aatattagca aaggatggaa atgacaatga
     4621 taaaactaaa tgataattta taaatatctc cttacgatat ataataattg tagggagatg
     4681 tttctttttt tgtatagcat aatttttcta gaaattctgt ttcaatatgt aaaaagttgg
     4741 tatatataaa tagtataaac agtgttgcta aaatatggta gatattatat aatataagtg
     4801 gatatgtata tacattatga gttatataat ttgaatttaa taaaatagaa acgttgattt
     4861 atgttctgta atgtgggcac cttggagcat attgagttag tggtgcaacc ggctatgaaa
     4921 ttgtatttat ttataaatac tattattttc atagcttttt ttatttaaaa aatggttata
     4981 tgttagaaat aaatttggtt agtacgttta aataatttta cttagatttt taaccagata
     5041 tattgtaagt aagtattaat taaaaaattt aagggggaat aaaaatgaag ttaaaaaaga
     5101 ataaaaaagg tttcacttta gtggaattat tggtagtaat tgcaattata ggtatattag
     5161 cagtagtggc agttccagct ttatttagta atataaacaa ggctaaggta gcaagtgttg
     5221 agtctgatta tagttcagtt aagagtgcag cattatctta ttattcagat actaataaga
     5281 taccagttac accagatggt caaactggtt taagtgtttt agagacttat atggagtctc
     5341 tgcctgataa agctgatata ggtggaaaat ataaattgat taaagttggt aataaattgg
     5401 tattacagat aggtacaaat actgaaggag ttacattaac agaggcacaa tcagcaaagt
     5461 tattgagtga tataggtgaa aataaaatat atacaagcac tacaacaaat agtttgggaa
     5521 atccattaac aagtaataca aaaatagata ataacgttct atatatagta cttatagata
     5581 atactgtgat ggacacaaca aaatagttaa aacaatattt ttataaaaat attgtaaatt
     5641 taatgaaaaa tataatataa aagacaggaa gaaaatcttc ctgttttttt atactatttt
     5701 ctttaatgtt caattatgaa tgatgatata attgagatag taaaggagtg aaacctgtgg
     5761 ctaaaaaagt tagaatagga gacaaactgg tcgaaaaagg atatataaca gaagaacagc
     5821 ttaaatgggc actatcagaa caaaaaaaca gtggaaaaag actaggagaa tttctagtac
     5881 aagaagggct tatagatagt aacctactaa taagtgtatt aaaagaactc ttggatatag
     5941 aaagtatatt tttagaaggg acagaaatag acactttagc tacaaagatg gttccagaaa
     6001 atatatgcaa aagatatact gtatttccat tcaaaataga tggaaataaa atatgtctag
     6061 ccatgtcaga cccacaagac agagaagcag ttcaagatgt aaggcgtatg tcaggtaaag
     6121 acgtagagat atttatatca agtacagaag atataaacaa agcaataggt catgcttatg
     6181 cacattcaga aataaataag gcaatgactg agtacaacaa aaatagaact ggtggagtaa
     6241 gagaaacagt aatacttgaa gaagatgtaa atgctgctcc aatagtacga ttagtaaata
     6301 acatactaga aaatgctgtt agaatggaag caagtgatat acatatagaa caaagtgaaa
     6361 attacatgcg tgttaggttt aggatagatg gaatgttaag agaatatatg aggatgaact
     6421 cagcaccata taaggcagta atcagccgta tcaagataat gtcagatata aatatatctg
     6481 aaaagagaat accacaggat ggtagaatct atttaaaagt ggataataaa cccatagatt
     6541 ttagggtttc tacaatgcca actaacaggg atgaaaagat agctatgagg gttcttgata
     6601 agagtaattt tatggttagt aaagaagtac taggaataga cgaacatggc tctaagatat
     6661 atgatgaatt gataaacact ccttatggtc ttatattggt tgttggccct actggaagtg
     6721 ggaaaaccac tacattgtat tcaatgttaa atcaactaaa tacagaaaat agaaatctat
     6781 taacaataga agaccctata gagtatgaat tacctggtgt aaatcaatct caaataaatg
     6841 aaaaggcagg gcttactttc gccagcggac ttagagcttt tatgcgtcaa gacccagata
     6901 taataatggt tggagagata agagatactg agacagcaga aatagcaatt agagcttctc
     6961 ttacaggaca tttagtttta agtacattac atgcaaatac agcagttgga gcaatttcac
     7021 gtcttttaga tatggatgtg gaaagttttt taataacttc atctgtttta ggtgtaatat
     7081 ctcaaagact tacacgaaaa atatgtgagc attgtaaggt ttcatatgaa gcagatattg
     7141 gagaaaaaaa ggctcttggt attgatgtaa atgaatcagt aactatttat agaggaaaag
     7201 gttgtgaaag gtgtaataat actggatata aaggacgtct aggtatattt gaaatgcttg
     7261 aaattactcc agaaataaaa gagttaatag attcatctgc aaatcaaaga gaaattttaa
     7321 agatggctag aaaacaaggt atggttagct taaaagagga tatagttaaa aaggttttaa
     7381 atggaaagac aacagtagaa gaaatgatta gaataatttt gatgactgat taaaggatga
     7441 tattatgaaa agttttaaat acacagtaat aaaagaggac ggaaaaaagc aaactgatgt
     7501 tattgaagcc aacgatttta atgaagctag aaatcttctt agaaagagaa atcttagagt
     7561 tattgaaatc aaagaaagca aaaataaaaa tataagatta tctagtataa agagaaaaaa
     7621 agacttagga gctgaccaga taagccactt ttgtagacaa tttgctatta tagtatcttc
     7681 tggtataaat agtatttctg gattagaaac attagctaga aggtcaacta acattacatt
     7741 aagagaagaa attaatcgta tagtagcaga aataaaagta ggttctacta ttgcagattc
     7801 aatgctttca tcaaaatcaa agtttccaaa gttacttggt gctatggtag ctacaggtga
     7861 agctacaggt aaattagatg aagtattaaa aagtatggct tcattttatg caagtgaaca
     7921 cagggttaaa caaaagctta gaaatgctgc aacatatcca ttaatagttt tgatttcttc
     7981 atttataatg atttttatat ttacaacatt tatggtgcct aagatgatta attcaattac
     8041 aacagtagga gcaactcttc cacttataac aagaattgtt atggggtttg gtatgtttat
     8101 gaagaagttt tggataatag tacttttagt tattatatta tttatctatc agtttaaaaa
     8161 atatctaaaa acgtcagttg gaagggctca taaagataga gttataaata agatacctgt
     8221 tttaggaaaa ggaataaatt gtatggtagc tacacgattt tcaagagcat tgtatctatt
     8281 tgtatcaaca ggatacccat tagttcaagg gcttgattat attatagata gtgtaaataa
     8341 cactatggca gagaagattt tagcatcagc taaagatgga ataacaagag gagaaggctt
     8401 agcagaaaat ttagagagat atacgtattt tgactctgtg ttggtacaga tgatagctat
     8461 aggggagcag acaggggaat tagagaatat aagtagacag atggcagaat tttatgagta
     8521 tgaatccgag atttatttaa ataggatggc ctcaatgata gagcctgttt taataattgc
     8581 agttggtatt atagttgcga tactggttgt atcaatattc atgccaatgc ttagtatata
     8641 tgatgccatg taggagggaa aggacaaatg aacaaggttt atgtatcata ttatggcgat
     8701 tctatttcca tagttgaggg cagatataaa cacgataagg ataagtttta tgtaaaaaat
     8761 tttaatgtgt tttcaataga ggatattgca caagattttt ctaaggaaaa gtatgctcta
     8821 ttgagatacg cattagaaaa tgttaagtta aaatcaagac atgtagtttt ttgtttaaat
     8881 acaagagatg taattttaaa accccaaaaa cttcctaata tggacaaaaa ggatttagac
     8941 ggatttatga agctagagat ggacgaaatg atggctttgg agtcagatga atatgtattt
     9001 tcgtatgaag tatctagtga aacacctgat aattcagatg gcgatgaatc aagcttaaat
     9061 cttattattg gagctataca aaaggaagag gtaaatgcaa ttgtagagat aatgcatgaa
     9121 tttgatttag tattagatag aatagataca ttatcaacag cttacttaag aatgcttaaa
     9181 aatttagact acaatgatat aatggtaacc aatataagtg acaatagcac aattataaat
     9241 atatataaga aagatacttt atttattagt gataatatac ctataaagat aactgctaaa
     9301 gatatggaca tgcaattatt aaatgtagta gaagaatcaa aaggtcttat gaattactat
     9361 tcgtcaagaa actttggtaa ggttacggat actatattaa tattaggaaa aagatattcc
     9421 aataaattaa tttatgaaac ttttagctct gcattttcaa gctatgtttc tcaaggcctt
     9481 actgagataa tggatatatc tgatatgata actggagata tagatgaaga tgatataaat
     9541 tcaattgtag agcttttagg aagtatgata gaacctaaag ggaaaagaga ttttgaaaat
     9601 ataaactttt taccaatgga tatacttaga aaacagataa aagctaaaca aatgaaaaac
     9661 atactgaaaa ttgctccaat agttattttg attcttgcta taccttattt atgtttaatt
     9721 ttttcaaata atttagcaaa taaggaactt gaaaatgtta atggtgaaat aagaaatatt
     9781 gaatctgcat attatcagat tggaaatata gaaaaaatga tacagagtaa aagcgaagaa
     9841 ataaaggtat atgatatgtt aattgggaaa aaggtaaagt gggaaaattt attggatgat
     9901 atagataaaa atactccaag taaagttgaa ctaacaagtt tatcaacaac ttatcaaaat
     9961 cttggaacta ataataaagc tgataattca aaaagtaata agactacaac agataaatca
    10021 agtaacaatg aaacaaaatc aagtacgaaa agtgatagta atggagataa aacagataat
    10081 gatttaaatg aagataagac tacaaatgtg tcagaagcat taactggaaa tagtgagact
    10141 aatagtaata acacggataa agatgataaa actccattgt atgataagat accaaatgtt
    10201 ataaatatag aaggaaatgc taaagattcc tcttatgtag gtcaattttt atataaatta
    10261 aagggattat actatttcaa ggatgttaaa ttacatagca taaaaagtaa tgcagaagat
    10321 tctaactata catttagtat gacattatac ctgaaagaag gtgtattaac taatgaataa
    10381 agatatattt aaaaaagata taaaagatat atttaaaatg gaagttagtc taaaagggat
    10441 tcttataaag gttataattt tattagctat tttatcatat ggttattttg gactttaccc
    10501 tcaatatcag aaatatattg ctacaaaaaa ttctgtaaaa caggctactg aaaaattaaa
    10561 tatatataaa gagaaaactg cacttatgcc aaaatatgaa aaacaacttg aagaagttga
    10621 aagtgaatta aaagaaaaaa gcaaaaatct tttatatgat atgcaagatg gtcttttttt
    10681 aataggttta gataaaaaaa tgaaggctta taatattgac ttaaccagtt atgacgttgg
    10741 aaatgttaaa gaatacgaac atttttatgg tatatcaaca acaataagtg ttagaggtga
    10801 ttatagaaat gtcaaaaaat taatggctta tttagaagaa caaaaaaatg ttactcaaat
    10861 actagattat acaatgactt catatataga accagaaaaa gaagaggaag aaacaacaaa
    10921 caataaggat ttaattaaaa atgtgaacat aaataaagat acaaaaatat attgggtgga
    10981 aggcgaaaat aagtggcatt tatataagga ttgtccattg ataaaagagg gagagattat
    11041 aaaaaatgac aaacttttag ataggtctac taattatcta tgtaatactt gtgtacttag
    11101 agctgcaaca gaaaaagaag caaaaaaaga agtcgtagct ccaaaggcag aaggtgtagt
    11161 agatgctaca tttaaattta taatgtacac taaagagaat actacaatga tacttgatac
    11221 agataatcca tcttcatgga gaccaggaaa atataatcca tttaagtcta gtgcgaaata
    11281 aagctaagta agctttaaag ataggtgatg agtttgtaca aaaataatga aaaaggtctt
    11341 actttattag aagtaataat agctgttttt atactgacaa tagttttaag tatttcttat
    11401 aaagtgttca atggtataac atcagcagta aaaaagcaac agattattac agatgctcaa
    11461 gtaaatatta atctaattaa taagtatcta aatagagatt tggaaaactg taaagagcta
    11521 actaaaactg gttcaggtaa taattatgaa tacaatatag agatgccaga taatgttgta
    11581 aaatatgaag tttctataga aactaaaaaa aatacagagg tatattctgt aacgagaatc
    11641 caaaacaata caattgatac agaaaatgaa gttagagaag agataatcta taatcaacca
    11701 ctagttcaaa acaataaaga aatgaaggaa acaccattta aaatagaaaa gcaaactggt
    11761 aaatctattt atactgtaag tatatactat aatgaatcag tgcaagaaag tcataaaaat
    11821 agcaatttaa ataacaaaac ttatacattt gatgttatgt caagaatagg ataataagaa
    11881 tttattaaaa aataagtagg ttgataaaat atgagtaaaa agaatagtga aagaggattt
    11941 tccttaattg aagtgttagt agctatggct attatgggaa ttgtactatt tgcatttttt
    12001 aatattatca atactaataa caaagcaaac actaaaaatg acacagatat aacttcctta
    12061 aactatgtcc aaagtgaaat agaaaatcta agagaaaaga taaaaagtgg agaatttgat
    12121 tttgatagtt tagataaact agaagatgga actgtcgtat atgaaaaatt aatagataaa
    12181 tcaaaaaaag ttgtatatga taaagttctt agtgaaggtg acgtgagctt atatgatact
    12241 ccatatgaaa agattacaac aataaaagat gaagatggta atttaatcga caaaggaaat
    12301 attacaaata agataaagac tattgtagaa gataaaagtg gtcagatata taaaatagct
    12361 gtaactggga aaagcatgaa tgactattca agtaaaaaag aggttaaaat tgtaactgaa
    12421 atatttaaag ataaataatt ttacttgggg gtgagatttt gaggaagtgg aataaattta
    12481 aaagtgaaag aggggcagct ttagttttag ttttggttgt agtagcattg ctttctattg
    12541 ttggattgat attttcaaat cagatagcta atagaataaa atctacaaag actactaatg
    12601 aaggtataca agcaaagtat ttagcagaga catgtgtgga aaatagtatt gataaagcat
    12661 atgaaaaatt gtatgatgag cttgagaaaa tggataatga gtttaaaagt gaaaatcaag
    12721 aaaaatccat atctaggtca aaattaagaa atatatcaga tgaagatttt aataatcaag
    12781 atgaggaaaa tattgaagca gaaagacttg gatatatgaa taatataaat ttctatttaa
    12841 ataaagcttc ttcagacttg gaaaaagcta gtatggaatt gaaaaagcta tatgatttag
    12901 atatgttgga ctatagagac attgaatatg tagatgcaaa tattatttca catagagatt
    12961 ctatcttaga aatttgcaag aattatactt ctggagatat tagtaaaata aatgaatata
    13021 ttctgaaaga agatatagac tcaactacat tgattgaggc aaagttagtg aataatgata
    13081 ttcttttaaa aaagttttta gaagaaaata aaattgaaaa tgaacattta aattcagcct
    13141 tttcacatac ttataaggca ttagataaca tttctttggc gatgcaaaat atgattgagt
    13201 atagacatac gtttcatata gatgagccaa aagtagaagt atctaatggg attccagact
    13261 ctcaacaata ttatgaacta atacaaaatc ctataataaa tagtatggag tatagctgga
    13321 atagtaaatg ggatacatta gaaaacctat tggaaatttt accaaatcaa actcaaggat
    13381 ttaattcttt aagagttcat ttgagaaata atgtaagaaa atttgagaag ttaagcgata
    13441 atatatctag tggtaaaaaa aatacagcaa aaaatttctt aaagtataaa gagttattgt
    13501 atgaaatatc agaccaatgt aatcaactaa aaagtatgtc ttatgagaaa atacctgtta
    13561 aatatgataa catggctttg ataacaactt ttgactatat acaaaatgag cttttagcag
    13621 agataaaatg cagattaaaa gaattaaaac cacaagaaat tgataaaaca gaaggtatta
    13681 ctataaaaat acctttttat aaggctgact acgacatgac aaaagaagga tggcctaaat
    13741 taaaagaaaa tggttcaggg gcggagttaa gcttaatggt aactggtgat aaaaatggaa
    13801 taaaagaagt ggaagttaca gatggtaaaa aaaatattat tggtttaggt gttgaagaaa
    13861 attcaaattc aaagtataag gttgacgcca ttgtaaactt taatctaaat atagatacaa
    13921 atgtagttgg aaattatgat ataaaagata aaattttgat taatcatgat ataagttcat
    13981 acaaaaaagt aaactaaata taagaagcaa cttagtataa ataacaaaat aattttatta
    14041 tttactacta aaaaaataag taaactaata gaagatatgg agagtaaata ttatgtatat
    14101 ttatgattta ttagagcaag gtataagact aaacgcatca gatatacaca taacagttgg
    14161 aacaaatcct gtagctagag taaaaggtgg atttgtaaaa ctaagtgaac aaatattgac
    14221 atcagaagta acaatgcaaa tggcaaaaga catagcaggg gaatctatgt ttaaagtaat
    14281 agaagaacat ggagaagctg atttttcagc ttctctaaaa acaggagaaa gatttagggt
    14341 aaatgcatat aggcaaaaag gaaattacgc aatagcaata aggactataa cagcagaaat
    14401 tccaacattt gaaaaattag gccttccaga gagtataaaa tcttttactg aaaagcataa
    14461 aggattagtt ttagttacag gtccaacagg aagtggtaaa agtacaacac ttgcaagtat
    14521 gataaatata ataaatgaaa aacaacaaaa acatataatt acacttgaag acccgataga
    14581 atatgtacat catcataaac aaagtttagt aaatcaaaga gaagtaggaa ctgatacaga
    14641 aagttttcat tcagccctta gagctatttt gcgtcaggac ccagatgtaa tactaatagg
    14701 agagatgaga gaccctgaaa ctgtttcaat agcattaaca gcagcagaaa caggtcattt
    14761 agtattttca acactacata cagtaggggc agctaagaca attgatagaa tagtagacat
    14821 gtttcaacca tcccaacagc aacaaataaa aacacagtta tctacagttt gtgaaggtgt
    14881 ggtatcacag cagttacttc ctacagcaga tgggaaaggc agaatagcag caattgaatt
    14941 gatgtttgct actccagcaa taaaaaacct gataagagaa ggcaaaacat atcaaattcc
    15001 aaacatgata caaactggag ttaaagtagg tatgaaaact atggaccaag atttaatgga
    15061 actttataaa aatggaaaga ttacaaaaga tatggcactt agtaggtgta ctgaccaaga
    15121 gtttatgaca agaatgatag gtggagtaaa ctacaatgga tattataatc gttaatctat
    15181 atgtatttat agtgggtata gtgtttggaa gtttctttaa cgtctgtatt tttaggattc
    15241 cagaaggtat atctatttct agaccacctt ctcattgtat gagttgtaat actagactaa
    15301 agccaataga cttaatacca gtgctaagct atttaatgag tggcagaaag tgtagatatt
    15361 gttatgagaa aatttctagt agatatgcta tagtggaatt aattacagga cttttatttt
    15421 taacagtatt tatgttatat ggaatatcta catcaactat ttattatttg gttcttgtgt
    15481 ctttactgat tataattaca tttatagata ttgaccactt tataatacca gataaaatat
    15541 tggtttttgg agctatattt tctatgattt ttaacctttt atttaaagaa attccagtta
    15601 aggattcaat tctaggtgtt tttatttgtg gaggaagtgt ctggattata gttcttttga
    15661 ttgaatttgt aataaaaaaa gagtgtatgg gtggaggaga tattaaacta tttgcaatgc
    15721 taggccttta catgggtgtt aaaaatggtc ttttgacagc cttactaagc gtttatgtag
    15781 gagcagtcta tggaatctgt gtaataatta taagtagaat aaaaggaaag gaatataatt
    15841 ctgttatacc atatggacct tttatatcta ttggagcatt aataacaatc ttatgtggaa
    15901 agaaattact tgaactttat ttttctatgg ttatatagat gatagtcaag ttaaataaag
    15961 ttttaattta aaaaaatgaa ttaaagtatt taataaggac aatattatta ttaaccctat
    16021 tactaaagtg ctacaaacta tggagtatat aataataatt tccttatttt ttgcacattt
    16081 gataaataaa tctctatgtg ttttttgtga tagagaaaag aaaaatttat acaatacgtt
    16141 ttttatttac ataataactg tagttttatc tgtattaata tatctaaaat ttgggattag
    16201 tgtagaatgt gtacaatata tggttttaat tccattttta ataattatat ccattgtaga
    16261 ttactacaca atatatgtat atgatataac aatagttagt ggtataatag ttcaaggtat
    16321 aatttttttg gcctttttca atatagctca atttgattat ataaatcata tatttggact
    16381 attgataggc tttattatac cttatattat tgctaaacta acaaaaggat taggctatgg
    16441 agatgtaggt ctctatagct tatgttgctt tgcattggga aataattatg ggttttatgt
    16501 gatagtttta tcatttattt tagcgttttt gttttttatt attttataca taactaaagt
    16561 atttaaagat gtagatataa gaaagcaaat tccttttaca ccttttattt ccttgtcgac
    16621 aattttaata atatttacag agtataatat atttaatttt tattataatt tgttatacaa
    16681 atttatataa caggaggtaa gaaatgtacg tagtagtagg gttaggtaat ccaggaaaga
    16741 agtatgagaa gactagacat aacgttggat ttgatgttat agatattctt gctaaagaat
    16801 acaatattag tgttactaaa ataaagcata aagctttgat tggtgaaggg agagtaggta
    16861 ctgaaaaagt attattagtt aaaccacaaa cttatatgaa tttaagtgga gaaacattaa
    16921 tagatatata taaatattat aaggttgatt taagtaatat agtagtagta tatgatgata
    16981 tagatttaga agttggaaag atacgtataa gaaaaaaagg aagtggagga actcataatg
    17041 gcatgaaatc tataactaat tgcctaggtt caaatgactt tccaagagtg agagtaggtg
    17101 tatctaaacc agaagctggt caagacttag cagactttgt actttctaga tttagaaaag
    17161 aagaatctga taacataaat gaagcattag aaaaagcggc agatgctatt gactcaataa
    17221 taagagaaaa tatagatatg tctatgaaca aatataatgg atagtgagag ggagatttaa
    17281 atgaatgatg tttttttata tcctttgcaa aattcaaaag agtataaaga tataataaac
    17341 tgtataaaaa atacaaaagg ctcattattg gttaatggac ttttaccagt acaaaaacct
    17401 catatatcgt attctatatt taatgattta tcacgacaaa tgatttttat aacaagtagt
    17461 gatttagaag caaagaaagt atacgaagat ttaagttttt atatggaaga taaagttgaa
    17521 tatttaggtt ttcaagatat ttatttttat cacttggatg caaaggacag aaacgaagaa
    17581 gcgaagaaat taaaagtatt gttaaaacta gcaaataaag aaaaaatcat tttagtaact
    17641 tctattgagg cagttttaag aaaatacata ccaaaacaag ttttgctcga tagtgtatct
    17701 cattataagg tgggagatag cttagactta gaaaagttaa ctgagaaatt agtttcattg
    17761 ggatatgaaa gagtttcaaa aatagagggg tttggtcaat ttagtataag aggtggaatt
    17821 attgatgtat tttcacttga atatacaaat ccaattcgta tggaattatt tgatgatgag
    17881 atagattcca tacgaacttt tgacgtatat tcacaaaaat caattgataa attgcaacaa
    17941 ttctctataa ctccatctag ggaatttatt tatcccgaaa aaactacaga tgcattagtt
    18001 aaattaaaaa aagaaacaac taaaaatact gatgaaggta catttcaaaa tatagattat
    18061 atatcaagta agacttattt tgaaggtgta gagaactata tagattatat ttatccagaa
    18121 gaaaacaaaa gtatatttac ttatttagca gatgatgcaa tagtatttat taatgatata
    18181 acgagactta aagaaagatg cgaaaattat ataaatgagt ttagagaaaa ttataaatta
    18241 aatcttgaaa gaggtctggc tattaaaaac caaggtaaat tgttatatca ctatacagat
    18301 ttagagtact tagtaaaaga caagtctgtg gtattaaact cgctattgcc aaagtctata
    18361 aataactttt ctataaaatc tataataaat tttgaaagta gagaagttcc tacatttaat
    18421 ggaaaggtag atttgcttgt agaagaatta aatcggctga aatataatgg atacaaaata
    18481 attttagcaa caaatacctt agaaagagca aataaattag gaaaagattt attggacaaa
    18541 ggcttagaaa cgaccatttc taaagataga gatattgaaa ttaaatcttc acaagttata
    18601 attgtacctg cacacataaa cagtggtttt cagtataaat ctataaagtt tgttgttata
    18661 acagataacg aaatgatagg tgtttataaa agagcgtcta agactagcaa taaaaaagtt
    18721 aaaaagggaa agaaaataga atccttttta gatttaagtg ttggagatta tgtagtccat
    18781 gaaaatagtg gtgtaggtag atacacagga attgaacaaa ttacagtcaa tgctattaaa
    18841 aaagattata tgaaaatagt atatcaaggt ggagataatc tatatgtgcc aatagaccag
    18901 atggacaaag tacaaaaata tattggtgca gaagtagaaa aagttaagtt aaataagctt
    18961 ggaacaaatg agtggacgaa agcaaaagca aaagttaaaa aagaaattga agatatgaca
    19021 aaagacttga ttgagcttta tgcaaaacgt gaaaaaatac aagggtataa gttttcaaaa
    19081 gatacacctt ggcaggcaga gtttgaatcg ttgtttccat atcaagaaac agaagaccaa
    19141 ttaaaggcta tagaagaaac taaaaaagat atggaatcaa gtaaggtaat ggataggcta
    19201 gtatgtggag atgttggtta tggaaaaaca gaggtagcta ttagaagtat ttttaaagct
    19261 tgtatggacc aaaaacaagt tgctgtattg gttccaacaa caatattggc ccaacaacat
    19321 tataatacat tcaaagagag gtttgaaaat tatcctttga gagtagaagt tttaagtagg
    19381 tttaaaacac caaaacaaca aaaacagatt attgaagatg ctaaaaaagg attagtagat
    19441 attttaattg gaacacatag aataatatcc aaagatataa atttaccaaa cttaggtctt
    19501 gtagtcattg atgaagaaca aagatttgga gtaaaacata aagaagcttt aaagaaaatt
    19561 aaatctacag tagatgtact tacactttca gcaactccta taccaagaac attacatatg
    19621 tctttaagtg gaataagaga catgagtgtt atagaagaac ctccacaaga aagacatcct
    19681 gtaataacat atgttacaga gagtaaggaa agcgtaattc aagatgagat agagagagaa
    19741 ttaagtagag gtggacaggt attttttgta tacaatagag ttgaacacat tgaagaaatg
    19801 gcaagtatga ttcaaaaact cgtaccagat gctagagtgg ctgttgcaca tggaagaatg
    19861 acttctaaat cattagaaaa tataatccta ggatttttaa ataaagatta tgatgtacta
    19921 gtctgtacta caataattga aactggtatg gacatatcaa atgcaaatac aatgattata
    19981 tatgatgcag ataaaatggg attggctcaa ctttaccaat tgagaggtag ggttggaaga
    20041 agttcaagac aaggatatgc atatcttttg tatgaaaaag ataaaacttt aagtgagata
    20101 gcggagaaga gattaaaagc tatcagagaa tttacagagt ttggttctgg ttttaaaata
    20161 gcaatgagag accttgaaat aagaggagca ggaaatattt taggttctca acaacatggt
    20221 catatggcag ttataggtta tgatttgtac gttaaaatgc ttaatgatgc aattaaaaaa
    20281 gtcaaaggtg agcctattgt ggaggaaatt gatgttgaaa tagatttaag tgtaaatgca
    20341 tacattccag ataattatat caaagatgag ttgattaaaa ttgaaatgta taagaaaata
    20401 gcttctattg aaaacaaaga agacatgttg gatatacaag aagaacttga ggatagattc
    20461 tctgatatac ctaaaccagt ccagacactt cttacaatag cttatataaa atcgttatgt
    20521 aagatattaa aaatagaaaa gataagacaa ttaaaagatg aaatattatt agttccaata
    20581 acaaagtata gaactaaaca gaaaataggt tataatatag ttacagaatt agaagaatta
    20641 ttagaaaaga tgtgcaaggt aaaataactt gtaaatttaa gaagggatgt tttaaatttg
    20701 aaaaaagtaa taacattggt aatagctatg attttggtag tatctgtgac tgcttgcagt
    20761 tctagtaaag gagaaactgt ggcaacagtt gaaggtacaa agatatcatc agatgaattt
    20821 aaaaaaacta tagctcttta taaagattct atggaacaaa cttatggaaa agatatatgg
    20881 gataaggaag ttgaaaaggg tgtaaaatac aaggacaagt ttaaagattt aattcttgac
    20941 caacttataa ctacagaagt aatatattct caagctaaaa aagataactt acttcctaaa
    21001 aaagaggatg ttgaaaaaag ttttaaagaa ttaaaagacg ctatgggtaa agatgaaaaa
    21061 tataaagaac aacttaagaa attaggtata gatgatgagt ttttaagaga ccaacaagag
    21121 aaagatttag caatgcaaaa ctatcaaagt aactttgcta aaaagactaa aatatcagat
    21181 gaagaaatga aaaaatacta tgatacacat aaagatgaat ttaaaaaaga tgaggtagaa
    21241 gcttctcata ttttattgaa aacagtagat gataataaca agccactttc agataaggaa
    21301 aaagctgaag ctaagaaaaa ggctgaagag gctcttaaag aagttaaatc aggtgaggat
    21361 tttgcaaaag tagcaaaaaa atattcacaa gatacttcag caagtgatgg tggtaaatta
    21421 ggattctttt caaggggtca aatggttgct gaatttgaag atgctgcttt ttctatgaaa
    21481 aagggtgaag tatctgattt agttgaaact caatatggat accacataat taaagtgaca
    21541 gacagaataa atgagcaaac atcatttgaa gatgctaaag aaactataaa agaccaactg
    21601 ttgaaaaata aatatcaaga acaaatagaa aaattaacta aagaagctaa ggtagaaaaa
    21661 gatgaaaaag taataaataa aatcacaatc taagacttaa aaaaagagta atatgcttaa
    21721 aaaagctata tctggataag atatagcttt ttttgtatat ttttcttaac aatggtaaaa
    21781 ataaacttaa agtaatttat ggagggatta aaaaatatga aagcaacagg tatagttaga
    21841 agaatagatg atcttggaag ggtagttatc ccaaaagaga taagaaagac attaagaatt
    21901 agagaaggag accctttaga gatattcaca gcaaaagatg gagaagtcat acttaaaaaa
    21961 tactctccaa taggtgaatt aaatgaattt tcgcaagaat atgctgaaac attagctgaa
    22021 gtactaggat atggagtgtt gataacagac ttagattcta taatagctgt gtctaagctt
    22081 cctaaaaaag attataaaga aaaaagtata agtgatgagt tagaagcaat aataagtgaa
    22141 agaacagtaa aatattcaaa agataaaaac attttaccat tattcaagga agaccaaaaa
    22201 gaatatacta gtcaagttat catgcctata attagtgcat ctggtgactg tataggttca
    22261 attgtagttg tttcaaagga taaagataca attgcagaaa gtgaagaaaa atctttaaaa
    22321 attgctgcta actttttagg aaaacaagtt caataatttt atcataaaga ccttttacat
    22381 gaaggtcttt tgaagttttt gtgtaaaata ttgaatatag cattaaaaat atggtatatt
    22441 aaagtttgtt atataaacag tatggaggtt atgttatgaa caataacaaa aataaagact
    22501 cctttctaaa aggagccctt atacttgggc tagcaggtgt aatcgtaaaa ataatgggtg
    22561 gattttttag gattcccctt ggaaacatga ttggttcaga aggaatggga tactatcagg
    22621 ctgcttatcc tgtgtatact ctatttttaa cattagctac tgctggattt ccaacagctg
    22681 tggcaaaatt ggtttcggaa aaagttgcaa ttggaaattt taaaggtgca aataagatat
    22741 ttaaggtatc acatacagtt ttattcatta ccggtataat atcattttgt atattatttt
    22801 ttggagccga ctacatagta acaaacgtaa tgaaaaatcc aggtgcgtta tactctatga
    22861 aagccattgc tccagcatta ctatttgttc ctgcaatgtc tgcatataga ggatattttc
    22921 aagggagaca agatatgact aagattgctg tgtctcaagt tgcagagcaa ttttttagag
    22981 ttgtattagg tcttactttg gcttacttgc ttatgaagag tctaggtcag acttttggtg
    23041 cagcaggtgc tatatcaggg gctacaatag gttcaatagc atctatgctt tatttggtgt
    23101 ttgcttatat gctagggaga aaagagcgta gagcagagat agatgcaagc caaagattta
    23161 aagatgaaag agtgtcttat atttttaaaa agcttttaac tgttgccatt cctataacaa
    23221 taggagcatc agtaatgcca cttgtaaata tgatagataa tgttattgta ataagaagac
    23281 ttatggaagc aggctttaca tacaaagtgg caaattccat gtttggtcaa ttaacaggta
    23341 tggcaatggc cacaataaat ttaccagcag tcataacaac tgcaatgagt atgagcttag
    23401 ttccagcaat ttctaaagca tatgcattag gaaataagtc aaaagctaga aaagatacta
    23461 agagtgcagt taaggtaact ttacttatag tattgccttg tgcgtttggt atggcttctt
    23521 tagcaatacc aataatgggt cttttatttc cacatgaacc atcttctgta ggaactattt
    23581 tgtttactct tactccatgt gtattatttt taggattaat acagacattg actggtataa
    23641 ttcagggaat gggaaaacca atagtacctg taattgcttt atgtgttggt atgctatgta
    23701 aaattgtaat aagttatact ttaacaggta taccagatat taatgtactg ggttcagcat
    23761 ttggtacagt tacagcatac tttgtggcag ccatgattaa cttactatat gttaaaaaac
    23821 acatgaatgt aaacttttct aaaaaagaat ttataataaa gccatttata acagttatga
    23881 caatgtttat aatggttaag ttgagttatg gtgctttagt tggattctta ggaaacagca
    23941 tatctacaat aattgcaata tgtgttggtg gaattgtata tgtagtagta atacttggaa
    24001 taggtggaat taaaaaagaa gagattctaa ctatgccaaa aggtgataaa ttatataaat
    24061 tgttaaaaaa agttaaactt ataagataat ataggaatat atatgcaaat aaaggaaata
    24121 ctatacaaat agtaaatctc cttatttgta taataatata aaaataaagt gaaatttgct
    24181 aataggagtg atgttatggg taagataagt attgtaggtc ttggaccagg agactattct
    24241 cttataagcc aaggtgtgtt agaatcttta ataaatagcc ctaaagtttt ttttagaaca
    24301 gagaaacatc caataattga taaacttaaa gaaactatac aatacacttc tttagactac
    24361 ttttatgaaa aagaggaaaa ttttgataat gtttatttga aaatatctga atttatcata
    24421 gaagaatcag caaagggtga cctagtgtat gctgttcctg gtcatccaag agttgcagag
    24481 aagactgtaa gtataataga gagattagcc aatgaaaagc acatagcagt agaaatattg
    24541 ccttctatga gctttgtaga cgctatgttt aactatttgg ctatagaccc atctgaggga
    24601 tttaaactgg tagatgcttt tgaaatagaa aattcatata tagacttgga tacaagtatg
    24661 ataataactc aggtatatga taaatttata gcatctaatg taaagttaaa acttatggaa
    24721 cattatgact ataatcaaga agtatgtata gtaaatggag ctggtgtcaa agatttagaa
    24781 agtaaaaagt ttataaagct ttgtgaatta gacagaaatg aaaacttttt tgactattta
    24841 actagcttat atatacccaa aagttctaaa aagatgtata atacagtaca tgacttagaa
    24901 ataatagtta atacacttag aagtccatct ggatgtgaat gggataaaaa acaaacacat
    24961 caatcattaa aaaattctgt tatagaggaa gcatatgaac tttgtaatgc aattgataat
    25021 aatgatatag atgaaatggt agaagagtta ggtgatgttc ttttacaggt tatattccat
    25081 tgtcagatag gaaatgagga gggctacttt gatttaaaag aagtagtaaa tggcatatgt
    25141 aaaaaattaa ttcatagaca tcctcatata tttagtggtg caaaattaga tatgtctaag
    25201 tttgagaaaa cttgggaaga attaaagaaa gaagagaaag gcgaaagtac tataacagat
    25261 ggccttagaa ggataccaaa acatttacca gctcttatga aggctagtaa agttcaacat
    25321 aaagcagcac ttgtcggctt tgattgggac aatgtagaag atgtatttaa aaaaattgaa
    25381 gaagaatatc aggaattact tgacgaacac agggtcggga atataaaata cataaaggaa
    25441 gagctaggcg atttattatt ttcgatagta aatctagcta gatttttaga catagactca
    25501 ggagaagcct tgaactgtac tacagaaaaa ttcataaata gatttgactt tatagaaaag
    25561 agtgcaatga acttgaataa aaaattagaa gacatgacac ttgaagagat ggatgagttt
    25621 tggaatcagg ctaaaaaaca ataaaattgt ggaaggaaat ttgtcaaaaa tctagaactt
    25681 ttatcacaaa gatagaagtc tatattataa acatttttag gaggtaaata tcgtgaataa
    25741 agctgaatta gtatcaaaga tggcagaaaa aagtggatta acaaagaagg aagcagaagc
    25801 tgcgttaaac gcatttatga gttctgttca agatgcacta gtaaataatg aaaaagttca
    25861 attagttgga tttggaacat ttgagacaag agaaagagct gctagacaag gaagaaatcc
    25921 aagagatcca gagcaagtta tagatatacc agcttctaaa gcaccagttt tcaaagctgg
    25981 aaaaggatta aaggatataa taaatggata attagaaatc ttgtgaaaaa gtgtggataa
    26041 atttgccaca ctttttctat agaaccaagg aaggtgataa ttatgagatt agataaattt
    26101 ttaaaagtat ctaggattat aaaaagaaga actgttgcta aagatgcatg tgataaaggg
    26161 attgtcacta taaatggaaa agttgctaaa tcttcttcag aggtgaatat tggagataca
    26221 ttagaaatac aatttggaga gaagaagatg aagtttaaga taaatgaagt aaaagagcat
    26281 gtattaaaaa atgatgcaaa agaaatgtac gaaattttag aataaaatta atagtcctct
    26341 agtatattaa tagtagtatt attgaaatat ttactggggg gatattatgg aacagaacat
    26401 aactttgaaa gatagatcaa agctggttat ttcgggagtc gagcacatat actcttttaa
    26461 tgacaaaaga gtagagctca aaacttctgt aggcgagatg gtaattgaag gcgaaaacct
    26521 agatatgagc aagctaagta tagacgaaaa tataattagc atagatggaa ctataaattc
    26581 tatggtatat gcaaagccta aaaagccaca agagagtttc ttgaaaaagg tatttagata
    26641 gatgatacct tttactcaag atgtaagcat tttttatgct actatttatg gtggaatact
    26701 cataggggtg ttgtttgatt tttatagagg gctaagaggg aattttaaat tcatcaatta
    26761 ttttgctata atatttgatg tattgttttg gtttctagct acggttataa tattcgtaac
    26821 aataaacctt acagaatttt ttgatttaag atattatcat tttgtggctt tgtttattgg
    26881 ttttatttta tactataaca ccataagtaa aattgtttta tcaattatca ataaaataat
    26941 tcgttttgtg agaaattctt ttaaaaaagt tacacattac atagtcagtt ttttaaataa
    27001 tttgtattac gttataatct attcactaca cctactattt gatattatat tttatatacc
    27061 aaacatattt atagcaacaa gaaaaagtat aaaaagaaaa tcaaataaaa aattaaagaa
    27121 caagaaaaaa tcaaaaccta agaagaaaaa aaataaaact aaaaaaaggg tgtaggaaaa
    27181 atgaacttaa gaaaaaaatt ttcaggacag attattgtta tcagtttatt tttaggaata
    27241 tcaattttta gtatgatgac aggatttgta tttgagtata caaaagctaa ggaatataaa
    27301 aaagaaatag caagtctaaa taaacaatta aaaaagactg aaatacaaat aaacagctta
    27361 aagaaagatg aaaaatcata tgaaggagac ttagaggata tagcaagaaa aagattgaat
    27421 atggtaaaac caaatgaaac agtttatgta gacataaatc gttgattaaa acttgctaat
    27481 aagtttacag atgtattgat tttaaaattt aagatttaat aaaaaagtca ttcagttaag
    27541 gatggctttt tgtttttatt gaggagggaa tttcataatg aaagtaggag ctatagatat
    27601 tgggacaaac tcaatgagat tattgatagc agattataga gatgggaatt ttataaacag
    27661 aaaaaaattt gttaatacaa ctagaattgg ttatggtgtg gatagtaaag gatatataaa
    27721 agaagatgct atcaaaagaa atataaaagc attaaaagaa ttttctgata agtgtaatga
    27781 tgaaaagtgt gaaaaaattt attgtatggg gacatctgct cttagagaca gtaaaaatgg
    27841 agattacttt gttaaattag ctaaagaaga aactggaata gatgttgata tagttacggg
    27901 aatagaagaa tcaaatttgg gctttaaggg tgttttagaa ggaatacaag gtgatgatac
    27961 tattattgtt atagatattg gaggaggttc aacagaattt attgtaggtg attcagaagg
    28021 aattaagttt aataaaagtg aaaatgtagg tgctcttagg atgacagaga ggtttttgaa
    28081 gcacgatctt attgaagatg aagagtttga agctatgagt tcatttatat atggtgagat
    28141 tgacaaaacg ttaaatcata taaaagacag aggattaaaa actcttatag gtataggagg
    28201 tacgattaca tcattatcag caatgaatca agaattagag atttactcta tggaaaaaat
    28261 acataatagt aaaatacaca aaaaagaaat agaagaaatt ctacaaaaat taataaagat
    28321 gacattaagt gataaaaaga acttaaaagg gctacaagaa aaacgtgcgg atattataac
    28381 tgctggtgta aaaatattga atataataat ggaaaaatta gaaatagaag aaataaccgt
    28441 aagtgaatac gataatttgg aaggcttaat atgtcaaaag tcaaaaaaaa tgtattaata
    28501 ttttttgatt tttaaaaccg attatgacaa aaaaaattac tcctctttgt tacaattctt
    28561 aatataaaca aaagaggagg tttttttatg caaagaagtg tagcagttgt gaagaataag
    28621 actgtaaaac caaatgtcat aaaattaata agcggatata ttgattttac tacggcggca
    28681 ttcgctgtga tgggattttt acttagtagg tctattatag tagactctgt agctccttta
    28741 ggaattgcct tttttatatg tgcatcaaag attgataagt acaagatacc tgtattcata
    28801 tcaacattat taggaatatt gttatcatct aatgattttg gaaatgtagc aaaatatgct
    28861 gtgtgcctta caatatttat ggctatgaat aaaaaattaa aattatcaga ttcaattgct
    28921 aaggttgcat ttataggtgc aataatacta cttccaatct ccatgggaca agctttcttt
    28981 tcaaataggt atgtatatgg tcttcttata gcaggagtag aatcaataat aatgtttata
    29041 tctatatatg tgttttctta tgggataggt ttaatggcca atgtcaacaa taggatgtct
    29101 gtacggacag aagaagccat atcaataagt ttactgctaa cctttagcat aatgggaata
    29161 ggagatatat ctctgtttgg aatatcaata aggaccattt tatcgaccgt attgatattg
    29221 ctgacctcaa tagtaggtgg agctaccatg ggagcatcta gtggtgtaat tattggtgta
    29281 gcatccattt taaataatat gacatcagct gtttatatgg ggatatattc tttttcagga
    29341 ttaataagtg gagcatttaa taaaataaat aaatatttct gtatactagg atatattcta
    29401 agttggacaa taatatacct atatacttct ggtattactt ctaatatgat gcaattaaga
    29461 gatatactat taggttgttt gatagtacta gtattgccag agcgattatt taataagatt
    29521 gaaaaattaa taaaaagtaa tgtggcttca aatgaaatcg tatacgatta tattatgaga
    29581 agcaaaaacc taactaatag cagattgaac agtatttata aaacttatga tgaccttgca
    29641 gatacatttg ataagataag agaaaaagac aaagtgctag accaaagaga catagctaat
    29701 gtaatagata tgattcataa tgatgaatgt aaaagttgta gtatgagaag aatgtgctgg
    29761 gagtcaagat ttaatcatac atacactatg atatataaca ttcttgaaaa aattgaggag
    29821 aaaggggagt taagtctaaa cgatatacct aaaaatttta gaaaggaatg tatgaaacca
    29881 gagtcaatag tgaaaatatc aaatcactat tacaaaatgt ttgtattaga ttatgattgg
    29941 agtgtgaagt tttcagaaag tagaaagtta atagcaaatc agataagaag tatttcaaaa
    30001 tccattaaat ccttatctca agatttagaa ggcgacataa tgctagatat agaaaaggaa
    30061 aaaaatatat atgaacagtt agaaagatat gatataacag ttgataaagt aagttactta
    30121 acaaaaagta acagtgagtt tgaaatatca atagaaaaga aaacttgcca tgatggatgt
    30181 atgtgtgaag ataaaattgt aaatataatt tcagacctag taggggaaaa tatgtctgtt
    30241 agaaaaatag gatgtcactg tttaggaggt aaatgcaagg caacattcgt taaatctcaa
    30301 aaatataagg ctgttacaga agtttcagca atgtcaaggg atgggcatat cctttgtgga
    30361 gacaattaca catacatgga gataaatgat ggtaaataca tgatggctat aagtgatggt
    30421 atgggaaaag gtaaaaaagc atatgaagaa tcttctgcca ctattgatat actggaaaaa
    30481 atgatagatg caaaaattaa agatgagata gtcattgata ctataaataa tatgttgttg
    30541 ttaaagtctt cagaagagat gttttcaact ctggatttgg gaattttaga tttaaaacga
    30601 ggttgtttag aaactataaa gatgggagcg tgttctactt atataaaaag agaggatgga
    30661 gaagttgact taatatcgtc atcatcatta cctgtgggta tattatcaga tgtaaaaatt
    30721 gatagaaaaa atgtaaaagt taaggaagga gactatgtta taatggtttc tgatggaatt
    30781 gtagatgcag gaagaaataa caatttagga gacaattggt taatatactt cttaaagaat
    30841 atagagacta caaatccaaa ggaaatttca aatttaatat tggatagagc attagagtta
    30901 caagcgttac aaattgaaga tgacatgact gttttagtta cgaaaatatg tacaaaataa
    30961 ggtttacaag attatgtgta taagatgtgg ataacttgtg tataatatgt taatagactt
    31021 ttgaaaaaag tatagttatt aacaaaaata acatataaat caacaaaatt tgttgataag
    31081 tatggcagaa atgttaataa aatgtaaaaa aagaaaaaac aaaagctatt atcaaaagag
    31141 attcatgttg aaataactaa atttctaagt aaagaaaacc tgtgaatatg ttgattatta
    31201 tgtggataac tattaattcg atatttattt atatagtatg aataattgaa taatatatct
    31261 aaaaaaagcc atcctattat taagtaaact tattgaaagg atggcttttt atgaatagaa
    31321 aattgtgcaa agcaataata cctttattta tttctatttt ttgcatgtta ataataagta
    31381 taaaagctga ccaaggatat caaaagaata atgacagagc aattactgtc atgaataata
    31441 atgatactga agatgatgct gaaaaaaaac aagaaataga ctatgaaaaa acatgttgga
    31501 atttgcaatc tttgtttaaa agtgatgacc aatggaaaaa agaacttaag agttttgata
    31561 aagatactaa agagttgaaa aattacatag gtaaagtaac aaaatcgcca acacatttgt
    31621 cttttgcctt aaacattaaa gaaaaacttg atataagact aaataaactt tctgcctatg
    31681 taaaattaaa acaagatact aataaaaatt cgtataagta cttagatatg aatgatagta
    31741 tgagtaaatc ctatgggaat tatatgggga tttgttcaga tttagagtta gaaatactaa
    31801 aattatcaaa caaagattac aagaaaatta tttcaaataa aaatataaat aggaaatatg
    31861 gtgcatattt aagagatata agaaggaata aagtacatta tttggatgat aaatcagaag
    31921 atattctaag caaagtatct tcaataagta gtcttccaag ccaagtgtat gaattattta
    31981 gaaatatgga tagaaaaact aatttgacac cagctcaata tgcgagtgaa ttagagtcat
    32041 cagatagaga aaatagaaaa aaagcttatc aagatgaact tataacttac aatgataata
    32101 taaatacaat tgcaggttta attactggtc aagttaaaaa gaatattttt tattctcagg
    32161 aaagaggtta tgagtcttct ttagaaatgt acctggaatc tgataatgta gatgaaaaag
    32221 tatacaataa tttgataagg actgtaaata gtaatatgga cagtctacac aaatatatag
    32281 atttaagaaa aaaagtatta aaattagata aggttcattc atatgatatg tttgtgccta
    32341 tggttaaggc tgttgataat aatattaatt atgaaaaggc acaaagtata gtatattcag
    32401 cgttgtcacc tttaggaaaa gaatacaatg atgtagtata taaagctttc aatgaaaaat
    32461 ggatagatgt ttattcacat gaaaataaag taagtggtgg atactgctta tctgtttatg
    32521 aaaatcatcc atatatactt ttaaattata ataattcatt aggttcagtt tcgactttat
    32581 cacatgagct tggacatgct gtatatgaat atttaagttc taagaatcag aattatttta
    32641 attcaaatcc gtctatattt actcacgaag ttgcatctac aacgaatgaa gcattacttt
    32701 acgagatgct tataaaagaa gctaaaggca atgatgaaaa agcttattat ataacacagt
    32761 atttagactt gataaaagat actctttata cacaaactat gtatgctgaa tttgaaaaaa
    32821 ctatacacga gttagtagaa aaaggaaaaa gtgtaaacgc attagtatta aatgatgttt
    32881 ggggacaact tcttaaaaaa tattatggac aaagctatga attagaccaa ttatctaagg
    32941 tcggatgggc tagaatccca catttttata atagtttcta tgtctataaa tatgctacag
    33001 gttgtagtgc aggagtaagt tttgcccaag acatcctaaa gaatggttca gataactata
    33061 tgagctttct taaaaaggga agttcagatt atccaataaa cttgttaaaa tcaagtggca
    33121 taaatttaac taatacaaaa cccatagaaa atacaatcaa aaaatttgat agtttagtag
    33181 cagaacttga aaagctatta taaacattat tgtaatccac atggtaaata tatcgaatta
    33241 atgataaaat atactaaaag tttaaagtcc tatcaagata ggacttttta tttattaatt
    33301 agtgggagat gattctatgc aagttaaagt taaaaaggct gtaataccag cagcaggttt
    33361 aggaactaga tttttgccag ctacaaaggc acaaccaaaa gagatgttac caatagttga
    33421 taagccaact ctacaataca taatagaaga agctgtggcc tcaggaatag aggaaatact
    33481 aataataaca ggtagaaata aaaaatctat tgaagaccac tttgataaat ctgtagaatt
    33541 ggaacttgat ttagagaaaa aaggtaagaa agaattgttg gaggtagtac aaaacatatc
    33601 taacatgatt aacatacatt atataagaca aaaagagcca aaagggttgg gagatgcaat
    33661 ttattgtgcg aggcatttta taggtgatga gccgtttgca gtaatgttag gggatgatat
    33721 tgtagataat gatgtacctt gtctaaaaca actaacagat gcatatgagg aatatagaac
    33781 tacaatattg ggtgttcaaa aggtaaatca agaagatact aataaatatg ggataataga
    33841 agcaaaaaat atagaaggta gagtttacaa ggtaaaagac atggtagaaa agccagagtc
    33901 aggaaaagct ccttcaaata ttgctatttt aggtagatat ataataactc ctgaaatctt
    33961 tgatattctt aaggatttac caccaggtaa aggtggagag gtacaactta cagatgcttt
    34021 aaaaatatta tctaagaaag aagctatgta tgcatacaat tttgaaggca agagatatga
    34081 tgttggggac aagctaggat ttttagaagc tactgttgac tttgctctaa agaaagaaga
    34141 ccttaaggaa gattttataa aatatttgaa acatgtatgt agtgaatttg ataaaaataa
    34201 caatgtgtta tacaataata cagatgaaaa aatagattta attgaagtca atgaagctag
    34261 agaacttgaa attcaaaaat aattaatgaa aggtgatgta tggtggcaaa atcaagtcag
    34321 aaaaaaatta atagaataat agctgttggg attgtgggaa tcctcgtaat aacgagtata
    34381 acagctgttt tgagtatgat tgcaactatg taataagata aatgacagca gttgatatac
    34441 ataaatataa aaaccatata taacttgatg tagtcaaaaa cttacaatat tttagttat
//
LOCUS       DAEOSP010000023        33376 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.023, whole genome shotgun sequence.
ACCESSION   DAEOSP010000023 DAEOSP010000000
VERSION     DAEOSP010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33376
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.023"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            229..1203
                     /locus_tag="KRH54_000865"
     CDS             229..1203
                     /locus_tag="KRH54_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578535.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     ISMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(1319..2380)
                     /locus_tag="KRH54_000866"
     CDS             complement(1319..2380)
                     /locus_tag="KRH54_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG8578536.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVANFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(2991..3842)
                     /locus_tag="KRH54_000867"
     CDS             complement(2991..3842)
                     /locus_tag="KRH54_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG8578537.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(3859..4779)
                     /locus_tag="KRH54_000868"
     CDS             complement(3859..4779)
                     /locus_tag="KRH54_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBG8578538.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(4889..5803)
                     /gene="murQ"
                     /locus_tag="KRH54_000869"
     CDS             complement(4889..5803)
                     /gene="murQ"
                     /locus_tag="KRH54_000869"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBG8578539.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(5902..6999)
                     /locus_tag="KRH54_000870"
     CDS             complement(5902..6999)
                     /locus_tag="KRH54_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG8578540.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLNFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(7232..8578)
                     /locus_tag="KRH54_000871"
     CDS             complement(7232..8578)
                     /locus_tag="KRH54_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8578541.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(8667..8975)
                     /locus_tag="KRH54_000872"
     CDS             complement(8667..8975)
                     /locus_tag="KRH54_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8578542.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(9237..9635)
                     /locus_tag="KRH54_000873"
     CDS             complement(9237..9635)
                     /locus_tag="KRH54_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578543.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(9637..10797)
                     /locus_tag="KRH54_000874"
     CDS             complement(9637..10797)
                     /locus_tag="KRH54_000874"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBG8578544.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(11228..12247)
                     /locus_tag="KRH54_000875"
     CDS             complement(11228..12247)
                     /locus_tag="KRH54_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8578545.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSENGSYTIFNIYFYDISDY
                     LSLDS"
     gene            complement(12241..12921)
                     /locus_tag="KRH54_000876"
     CDS             complement(12241..12921)
                     /locus_tag="KRH54_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8578546.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
     gene            complement(13073..14976)
                     /locus_tag="KRH54_000877"
                     /pseudo
     CDS             complement(13073..14976)
                     /locus_tag="KRH54_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(14977..15729)
                     /locus_tag="KRH54_000878"
     CDS             complement(14977..15729)
                     /locus_tag="KRH54_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8578547.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(16045..17199)
                     /locus_tag="KRH54_000879"
     CDS             complement(16045..17199)
                     /locus_tag="KRH54_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8578548.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSFLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(17202..17669)
                     /locus_tag="KRH54_000880"
     CDS             complement(17202..17669)
                     /locus_tag="KRH54_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG8578549.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(17683..18171)
                     /locus_tag="KRH54_000881"
     CDS             complement(17683..18171)
                     /locus_tag="KRH54_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578550.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(18185..19516)
                     /locus_tag="KRH54_000882"
     CDS             complement(18185..19516)
                     /locus_tag="KRH54_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG8578551.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(19530..21095)
                     /locus_tag="KRH54_000883"
     CDS             complement(19530..21095)
                     /locus_tag="KRH54_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG8578552.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSPATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQAYE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(21265..22050)
                     /locus_tag="KRH54_000884"
     CDS             complement(21265..22050)
                     /locus_tag="KRH54_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG8578553.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(22217..23416)
                     /locus_tag="KRH54_000885"
     CDS             complement(22217..23416)
                     /locus_tag="KRH54_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBG8578554.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKENKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            complement(23938..25275)
                     /gene="xylA"
                     /locus_tag="KRH54_000886"
     CDS             complement(23938..25275)
                     /gene="xylA"
                     /locus_tag="KRH54_000886"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBG8578555.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFSGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(25371..26921)
                     /gene="xylB"
                     /locus_tag="KRH54_000887"
     CDS             complement(25371..26921)
                     /gene="xylB"
                     /locus_tag="KRH54_000887"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBG8578556.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVILDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSKVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            27129..28289
                     /locus_tag="KRH54_000888"
     CDS             27129..28289
                     /locus_tag="KRH54_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG8578557.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(28445..28849)
                     /locus_tag="KRH54_000889"
     CDS             complement(28445..28849)
                     /locus_tag="KRH54_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8578558.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(28870..29352)
                     /locus_tag="KRH54_000890"
     CDS             complement(28870..29352)
                     /locus_tag="KRH54_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8578559.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(29372..30181)
                     /locus_tag="KRH54_000891"
     CDS             complement(29372..30181)
                     /locus_tag="KRH54_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG8578560.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(30174..30953)
                     /locus_tag="KRH54_000892"
     CDS             complement(30174..30953)
                     /locus_tag="KRH54_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8578561.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(30953..33031)
                     /locus_tag="KRH54_000893"
     CDS             complement(30953..33031)
                     /locus_tag="KRH54_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBG8578562.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
BASE COUNT        10974 a         5877 c         3571 g        12954 t
ORIGIN      
        1 gctatttaaa atcaatttta agttttcttc ttataataaa cttcctcttt gtggtctcat
       61 gttagtctct aaatttaaaa ttttacaaaa aattttcata aaataaaatt atattgattg
      121 tatctaaaca aatagggcaa taaaataatt aagtatagtt acttaccttt caagcaatat
      181 tagttttatg ttagaatttt attatattga tttacaggag gtttgcattt gaataatatt
      241 aattcactta aagtaataaa attatctctt ggatgttctc ttgctatatt tattgcttgg
      301 ctattaaaat tagaatactc aatggttgct ggagttatag tactacttac agtaaaggac
      361 actaaaagag aaactcttaa aggctccatt ggaaagattt atggttttct tttatgtact
      421 attttttcat atttatgttt taatatttta ggatataatt tgacttcatt ttcaatattt
      481 atatttataa ttataccact ttgttttttg ttaaatattc aagatgttat tgctatgtgt
      541 gtagtcatag catcccatta ctaccttcaa ggagaaactt ctatcaaatg gattttaaat
      601 gaagctggaa tatttgcagt aggaactgga ataggtgtct taataaatat gtatattcca
      661 acaaatatac ataaaatcca tgaagttcaa aaaaagttac aggaagaagt gagtattgta
      721 cttatagaca ttgcagatat tattgtaaat cctaaaaaag aaaatggata tagtagagat
      781 ttatatacat taaattcact tattgacagc agtatttctg aaacttacga taatattaat
      841 aatactttat taagtgatac tagattcttc ttagagcata tggatattat aaaaagccaa
      901 cgggatatct tagaaaattt gtatagttat gtatctcaac taaattctac accaccacaa
      961 gctcatattc tatctgcttt tatacataag ataggttata ctgaatttga agctgaaaca
     1021 ggcaatcttc ttcttgaaga attaaagaga ctaatgatta gtatgaaaaa ccagcctcta
     1081 cctgttgaca gaacagagtt tgaaaataga gctattttat tcctatgctt gactgagtta
     1141 aaacagtttt tagttaatcg taaacatgct cagatgctta gagataataa tttttataaa
     1201 taaaaagggg agaaatctag cagttgacct gactacaaaa tatcagatat aatattaaat
     1261 gcgcccctag taagagggcg caatgtttat aacttattct actgtcatga ttaaatcatt
     1321 attcatatcg aacatacatt atttgaccaa gtccaatatt agctttttca tcagtgattt
     1381 ctattttaat agtcacatca ttttttttac agtacttcgc taattctgca ccagaacgat
     1441 ttttaccact aaaatctaaa gaaaatggct tatcatattt aaaaggatat tcaacaggtt
     1501 taccctctgg tgctaatgca aacatcccat atggtgtgtt cataaatcct attacagcaa
     1561 tcgtaagatt tgatgggtct aaatttgatt cactacctgt aaatgtaatt gtatttactg
     1621 atgaattaac tacactacat ctaacacctt catacattac atgtttttca tttatatctt
     1681 ctcctttttt tccaaaattt gcaacagcct ttgccccttc ttgatgtggt aacatagtgt
     1741 gcttcccagg tggtgctggt ttctgtgtct gttctggttt taccgtctgt actattgcac
     1801 caacttttgg atatgctgtc ttatcaaatg gtaatgggtg gctaagactc ttgtatatga
     1861 tatcctttcc tgctgattta ttagcatcaa tatctacatg atacaaacgc ccatcaccaa
     1921 aacggataag attccactgg tggtcatttg atgtgctgtg tacataataa gattcaattc
     1981 ccatagcatc acacaaagct ttcatgctcc tagccattcc tgagcatgca ccttttttag
     2041 taaccaatgt ataggcagta tttgctccct ctactgcatt ccagtcaaca tatgttgtgt
     2101 tatttttaat gtgattgtag gcaactaaaa tcttagcatc tgtggtcata ttctttgtga
     2161 ttttcttgtt cataaaatct gctgttgctt gtgctactga gtttagttgg gcttgtgaca
     2221 gctttggacc atatacgcta ttggttccag tgacatagct ttttggtgtt tttgctgtta
     2281 cctctttggc tgtcatacca taaactgtca tactcatacc tgagattaaa actattgttg
     2341 taatcaataa cttggtagct aacttcctaa ttttcttcat acttaatatt ctcctttata
     2401 aaacattgtt acttcctatt ctctaccatt aataaaatta atttaggatt taaaaaggtg
     2461 gattgtcaac atgtatattt ctccattcat acattcgtga aatatttaaa tttcatggaa
     2521 aatctaatag atattttcta gataatttgt atacaataca actcgcaaat cagggtgttt
     2581 gctgttagtt tgagaaaatg atgatgatta atacattcgg aatctttgac ctataataaa
     2641 taccattgtc ttcaatcatt aataatggta agagtaaaga gaattatcaa taatacagtt
     2701 aaaatcaact ttgtatacat ttcatacatt ctctcccaac aacattatac ttcttataat
     2761 ttgtatattc aatacgtttt ctgggaaaat atagatatat ctataaaaat ataaaaaagg
     2821 agttgaactt aaaatttgat taaaaaaaat caattttaag acaactcatt tgtattataa
     2881 aaatgctata tattattatt aagtaagtca atattttaga tatttatcta tttcttattg
     2941 acttacgctt atagtctaac ttatttgttt agttgatata taacctagtt ttaagcatta
     3001 ttaatattta aataatcaag tatccttttt gtttcttgta gtttctgttc tgcctcattt
     3061 aagttttctt gaattataga aatatatatt atatctatca acgtaagcat tgctattctt
     3121 gaagaaatag ctccttctct aagtcttttt tctatatttg gtactactag ctttaaatca
     3181 ctaatcttac taagatgatt gtcactatat ttagttatac aaatgacctt acatcctttt
     3241 aattttgcat tttctataga ctttattact tcttttgatt ctccagagta agatattgct
     3301 atagctatat cttctttatc tacatttgaa gataccataa gttgtgtatg actatctaaa
     3361 gaggtaaatg cctgtttatt tatccttaaa agtttcatct gtaaatctag tgcaactaaa
     3421 gccgaaccac ctactccaaa tatatataca tttttagcct ttattatctg ttcaactgct
     3481 tcttttatta tatctttttc attcaacaac cttgtttggt tgatagtctc tatgtttttg
     3541 tttgtaattt tgtttataac actatctata ttacaatcat agttttcatc tacatatgtg
     3601 tattcatttt ctttgcccat caaatcttta gctatctcaa tttttaaatc cccaaaaccc
     3661 gaatatccca tttttctaga aaacctaact atacttgctg tacttgtttt tgagtttgac
     3721 gccaattctg atacagaaag cttacttata gcctctgtat tattaagtat gtaatcagct
     3781 atttttttat ctacttccgt taaatcttca tatatctgtt ttaattttaa aatgcaactc
     3841 atactctacc acctctacct atttactata atatttcttc ttagcaagta aacatgctcc
     3901 taaatctgga gtcaacactg gtctcactat accaaatctc gttttattca aatatttttt
     3961 aagtggctct aatattaaat ctcctgactt aaacactcca cctgaataag atacctttat
     4021 ctttgtacct tgttcaaaat gatgctctaa tcctattata agcctactta attcataagc
     4081 tgcatcatca aatattttac tagcagtatt atcaccttca ataactgctt ttaaacatat
     4141 cgtagcaaac tttgctattt ctattctatc tccttgtatt tcatcattta catattttat
     4201 tatgtcataa tcattctcta tattaagatg tctttttatt aaatcatata attttgtttt
     4261 tgaacacctt ccatcagact gcattgtgaa catttttaag gtagccattc ctatataata
     4321 cgctgaacct tcatctccta atacatagcc ccatcctcca catctgtgaa gattcccatc
     4381 tttatctaaa gcctgtgcta ttgaaccagt acctgcaatt atatttatac ctttttcccc
     4441 atttaaagaa cctgctaatg ctatttctac atcattacct agagtataat ttatatgact
     4501 atatgcattt tttgttgcta cttctaatgc atacaatact tcttttattt ttccatatcc
     4561 agcaagtcca acaaatgcat acgttatttc ttctacattt atcttagcat ctttacaaac
     4621 ttcttccaac ccagttatta ataatttcgt tagattatca aatcctattt gattataatg
     4681 acaggttcct ttggttatgg ttcctactat atttaattct tcatctacta atgtaaatgt
     4741 tgttttcgtt cctccaccat ctacacctaa aaaatacata ctatcctcct acaatatctt
     4801 taataattct ttcaatactt aatatctaaa aataaccttc caatgctgtt tttttaatat
     4861 tgaaaggtta tcagtactat tatttatttt acaatttttc tattgatttt aaagcttcac
     4921 ttatataacc ttcttgtcta tctaggatac tctttgcact atccttatct aaaccacttt
     4981 ttatcataaa tatagccaat ttaacgctat tatctgcttc ttctaagaat ttttctgcaa
     5041 catcatattt tacatttgta gctatcatta ttattctttt tgctctttca accaactttt
     5101 catttgatgt cttaacatct accatcaagt ttgagtatac ttttcccatc ttaaccatag
     5161 aagctgttgt taacatatta cataccattt tttgagctgt accacttttc attctagttg
     5221 agcccattat tacttctgcc cctactataa tgtttattgg taaatccgct tttttactca
     5281 tttcactatt cggattcata gttattgata gtactgcaca ccccaattcc ttagcatact
     5341 ccattgcacc tataacataa ggagtccttc ctgatgcagc tataccacat ataacatcct
     5401 tagatgtaac tcctctatct attatatctt gctttccaag ttctttatca tcttcagcac
     5461 cttctattgc tttaaatatt gcttgatttc caccagctat tatagcttga acaagctcaa
     5521 aatccactcc gtaagttgga ggacattctg tggcatctag tattcctatc ctaccactgg
     5581 tacctgctcc tatatagaat aatctacctc cctgtttcat tctatctact attatattaa
     5641 cagcttttgc tatactctcc ttttgttctt caacagcata ggctacagtt ttatcttcgt
     5701 tatttattaa ttctattatg cctaatgtat cttctttatc tatttgcaat gtattcttat
     5761 ttctaccttc tgtaactaaa ttttctaaag tttttattaa catatcattt cctcattttc
     5821 ttaatattaa atttttaaac acattttaat tccaactttc ataattatat ttttataata
     5881 aattaatatt ttcataataa attattttgt tgagggttta aaattaaact tttgccatgg
     5941 tttaatataa tctaatataa ataattcttc ttttcttata tatcctacta cattcgattt
     6001 accactgttt ttcatatcag tcaaggctat ttgtaactcc cctgcataat gaccatattc
     6061 actactttca attactatat ctcctctttt tattatttta ggagtattaa atagttcaaa
     6121 tttgtatcct ttatatttaa cacgagattg agtagacctt atcagactgt cagatatgtc
     6181 ccctctatta aaatgtattt cttcaaagag tatcttcttc tcaacttctc gtatattttc
     6241 ctctattgct acatcaaatg taaccatatc ttttctcata gaacctaata cctttagctc
     6301 ctcatatgtt ggataacaat ttgatattat tatatcattt atattattca tagctattat
     6361 atgctttact tgaatatcta taggtagatt tctgtgcatc tcaattgttg gaagcccatc
     6421 agttacaggc catggtccaa atgtagtttc attttgactt gttacaaaaa ctgctgttct
     6481 taatccatac ttattaaatc tttctgtaca ttttataaag tgttctaaat ttaatccact
     6541 atatctatgt ggataaaagt tatgacatcc tatcaaattt tctttatttg gttggtagtc
     6601 cattattgta tctatataat tagtattatt actcatattg atttctattt taagattttg
     6661 aggattaaaa gtcattaatg cctcttcaga acctgtaaat cccatatcaa gccttattcc
     6721 gtctgccttc gtctttttaa aaaaattcaa atctttgtag cttatttcta attcaccaaa
     6781 tactctggga tttacatcaa gtattacctc aaaaccttta aacttagcgt attcatttat
     6841 acttttaaat tttttaatta tattttcttt agtatcttta actgataata aacatgaaaa
     6901 tatacgtgta aatccatttt cagcagcttt atctatataa tcatatattt ccttagatgt
     6961 tgttttttct ggataaattg atattcctaa tcttcccatc tgtgtcatct ccttatatcc
     7021 tatatgtgtt taaatttatt tcgaattaca cgaaactttt ttaaaaattt gatgaaaaaa
     7081 agtttcatat atatttataa aaaaagaaaa gtttaccttt tcttttttta taaacaatca
     7141 acaaaatatc ttcgttactt atttcataac tgttatactt tgaaatttat actaatctaa
     7201 tatagttttt aatttccaat atagtttcta attaataagc ttcttgctct tcaacttttt
     7261 cttcatgaag caacctctta tcgtacatct tgacaaatgg atagtaaatt attattgata
     7321 tagctataca taataagtta agaactgaag ctctccaatc tccaccagtg gctaaatatg
     7381 cccctactgg tcctggtaat gtccaaggag cagtaactac tactctggat actaaattag
     7441 cactcatagc aaaccatgaa actgttgcca ttactattgg tgccacaaca aatggtatta
     7501 ttaatattgg gtttaatact attggtgctc caaatattat tggttcattt atattgaata
     7561 ttgctggtat tacagttgct tttcctaaag actttccata agctgatttt gctctaaatg
     7621 caagaagtaa agctaagcct attgtagctc cagaacctcc aatccatata aaccattgaa
     7681 aaaagggttc agcagcaata cctggaactg cttgacccgc agcatatgaa gctgtattag
     7741 catctaataa ttgtatccat aaaggtcttg ccaatgaacc tattattgat acaccatgta
     7801 ttccaaatga ccaaaaaaac gttattaaaa atattagtat aattactgat ggtaatgtgt
     7861 cagtggctga aactaatgga gttactaatt tagctatgaa gccatgccaa tcaaacccta
     7921 ggaaataagt aacaatagcc attaaaatta ttattaatgc ggctggtgtc agagcttcaa
     7981 aagaccttgt gactgatgct ggaactgctt ctggcatggt tatcttgaat ttagatttgt
     8041 cagtaaatct ataaatctca acacctatta tagatgttac aatagcaaca aacattgaac
     8101 ctccaccaag attagccatt ggcatttgcc atccttgagg aattgaatta taccaagcta
     8161 aaagttctgg actcttttca actatcttta ataaatcagg ggtaagaccc tctattgcag
     8221 taggaactat tgtcaaaagg aatgtagctg tagctaataa acctcctgtc aatccatcta
     8281 acttatatga ctttgccaaa ctataaccta tgccaaatgt agaatatact gatataataa
     8341 aaaatgtcat tctatatggt agtaatattg taactgcatt ttcctttaga tactgagtta
     8401 ccccccatga atctggtaat ggaggaaatg ctaatatcaa gaaaaatgac ccaactatta
     8461 taagtggtaa tgaagctacg attccatctc ttacagctct taagtgacgt tgttctgcca
     8521 atctggccat agaagttgat aatttagtgt ctaaaaattc tgtaaattta tctagcatct
     8581 tttctctcct taattaatat ttatttttat tatcattaaa atttttataa aatcaacttt
     8641 cttctaatct aacaacactg caactactat ttactcaaca gttcttgaac ttgttttaat
     8701 aactttggtc ctcctaatgg actataagct tgtgcttgta ttaatgcaca tggtacacta
     8761 acctcatcag caaaagcttt taaatagtca aatctatgtc tgacttgtgg agctaccata
     8821 gcaatatccc atccattttt aacttcattc tcaaactctt gagtaccaac agccaaaaca
     8881 tctatatcta ttccattttt tgctccttct ttttgtaatg cattaactgc tatagcactt
     8941 gacatccctg atgaacaaac aaataatact ctcatattga ttccccctgt ttttttattt
     9001 cttttcaatc attatagcat gtatttttga ttacaggaat acgtttgtat atattttttc
     9061 atattttaca ttttaaattg taattttatt tcatgtattt atatcttgct ttaattgctt
     9121 tatttatcta atcattcttt tgtctaaaat tacacactac ttaaaatact catatctttt
     9181 cttaaaaaag aaaaaagact atcttattat taacataaaa tagtctcaat acttttttaa
     9241 atatgtattc ctttatatat ccagaagtaa cctattattg caaatgataa agcaaataaa
     9301 atatacagtc taaaaaatcc caaagaaaaa cctctcttag tttcaacaaa gtatcttgta
     9361 tatgcaactg caaaagatgt aattaatgaa tttcctataa caactccaaa tctattatct
     9421 attccaaata tagataataa caaaggaaaa actacagtac ttactagaga tgaaactatt
     9481 aatattaatc cacttttaaa acttatagtt ggaatattat aatctgttct ttgatttttc
     9541 tccatttgtt tcttagtatt tttattactt tttgtacttt ttgcattttt ataattatta
     9601 tttttctttc ttttcttttt tttattatta gacatgtcat ttacctccaa aaggtttagg
     9661 tgttatattc cctaaaataa ctttattttt agcccctgtt gcagatatta cattagaaaa
     9721 agaattattt aatgtttcat tgcccaagat tacaaatgcc aaagcctcct ttgcatcaga
     9781 agaataccca tattcatctt gagttaatac ttcaacctct aataataact cttttatata
     9841 tcctattaaa gttagattat gagctcctcc acctccaatt aataaagtat ctacattaac
     9901 ctttggaagt atgaagtttt tataattata atatatagct tttgccgtaa acatagttac
     9961 tgttgctatc aaatcatatt tatctacatg tttatatttg ttaagcatgt tatccaaata
    10021 aacttgtcca aatacctctc ttccagtgct tttaggtggc gcttgactaa gatacttatg
    10081 actcatcaaa tcttttaaca tttcttcatt aattttacct tttgatgcaa agtatccatt
    10141 tttatcatac tttctattaa ataatctttg acatactcca tctattatca tatttcctgg
    10201 tcctgtatca aatgcaaata catcatctat gttacatgat ttaggtatta tagtaatatt
    10261 tcctattcca ccaatatttt gaagtgctaa atttttattg ttatcactat ataataaaaa
    10321 ttcactatat ggtactaatg gagcaccttc tccacctgct gctatatcca ttactctaaa
    10381 attagatatt acctttgtat ttgtttcata tgctataact gctggttcac ctatttgaag
    10441 cgttgaagat acaaaattat tataacttct tggtatatga aagattgttt gaccatgaga
    10501 tgcgataaaa tccaaatttg ctatatgaaa gtttgcttta tgacacactg attttacagc
    10561 tttactaaat aaatatccaa gcttaaaatt taagctacag attaaatcaa cactcgattc
    10621 ctcttctata cagcactttc ttatttcgtc ttttatatct ttaggaattt ctagagttat
    10681 aaattctatt tgcttaactt tagtatttct accattccca ctaatttcag ctagtactgc
    10741 atcaactcca tctaaggaag ttcctgacat taaacctaca ctatacatag ttatcatcct
    10801 tttaagttta tagttattcc tccccaaatc aaggtattaa gtttaataat tttagaatta
    10861 atcttatttc atctttaatt acgataattt actcttatga aattaattga gttaatctta
    10921 tacacgtttt ttaccttagc taaatcttac attaatttat tttcagtgtc aatgtaatac
    10981 tatgtaattt tttttcacaa acaaaacata ttctctctcc aaaaaccata ttaaaatagt
    11041 tactacaaat aaaaaacata ccaggtacat atctgatatg tttttcatat ataataatta
    11101 ttattttata atatagatag aacatcattt aaggcactaa ttaaatcttc actattttca
    11161 agaactaaat ttattcttat tgtatatgtc gatttgtata tgatgataga ttttatagtc
    11221 taataaccta actatctagg cttaaataat ctgatatatc ataaaaatag atattaaaaa
    11281 ttgtatatga accattttcc gaacatacct caatctcatg ccctaaacta tctatcattt
    11341 tctttacaaa atacatcccc attccagtag acttatacaa ttgatttctt ccatttttcc
    11401 ccgtaaatcc cttgtcaaaa accctagata tatcttcctc atttatacct ataccaaaat
    11461 cttttataga taataccact ccattttcta acttttttga attaaattca atcttaccaa
    11521 cttcctttga atactttata gcattattaa taatttggtc taaaacatat gacatgcatt
    11581 tagaatctgt gtaaacatca ttttctaatt cattcaaact aacttctaca ttatttttta
    11641 taagcaaaaa tgaattattt tttatagaca tttttaccaa tttttctaaa ttaaatttat
    11701 ttataaatac atcttcaaac atagtgttgc ttctactact atacaaaata gagtttacta
    11761 aaaaatttat ctttgcaacc tgattcttaa tatccaaact atcttctatg ctacttaacc
    11821 tatctgtaat tatacttaaa gaagatattg gaatttttat ttcatgaacc catttagcta
    11881 tgtactcatc caattctttt aatgattgtt tataagtctc tatgtcacta ttatttttta
    11941 tttttaagtt ttctataata tcttttatta gattttcttc aaaagattgt ccatcaacta
    12001 aataactgtc tatttcacca ctattttcta tacaattata taaatttgtg taactatttc
    12061 taaaatttat ataatctata acaaaaaata ccattattat aaacagcact aaaaaatcta
    12121 aatatattaa ctctgaatat ttatttttaa aaatatctat agcaaataag tagatattta
    12181 ttatactaac tacaactaaa ggtagagcaa tatacttaga cctacctttc aaatatttta
    12241 ctagcattgt attatatatc ctatcccctt cttagttttt ataaaatcat caaaacctag
    12301 ttctttaact ttacttctca atctattcat atttacagta agtgcattgt ctgtcacaaa
    12361 ctcatcatta tcccatagag tcatcataag actttccctt gatacaaccc tattcttatt
    12421 ttttaataat aatgttaaaa tttttatttc gtttttagtc aactctatac ttttatcctt
    12481 atacttatgt tttataatcc ctttccccac atcaaacatc aaatcttcac aatacaaaat
    12541 actattagaa tcactataat tatagactct tcttattata ccatttattt tggtaaccaa
    12601 tatttctata gaaaatggtt tggtaatgta atcatctgct ccattattta tacccattat
    12661 taaatccata tcagaatctc ttgatgataa aaatattaat ggtacatttg aaatagctct
    12721 tattttttcg caccagtaaa atccattata taatggtaaa tttacatcta aaagtactac
    12781 atctggtttt ttatctataa attcatctaa tactacctca aaatcatcaa ttaattctac
    12841 tttaaatccc catttttcaa gtgctaatgc aatctctttt gataaatcaa tatcgtcctc
    12901 tactaataaa attctataca tttgtatatt ctccattatc ctaatatttc actatgctat
    12961 gaattattaa tatcataaca tattttatat gaatttatta cccaaattat ttagttaatc
    13021 aacaaggaga ctcttattac aagaatctcc tttctcttta ttaatatatc aactaatttt
    13081 tatacactac actcttaaac ttattaacag atactgctcc atatattatg aatattatca
    13141 aacatatcac taaacttagt atatatatac caaataaatc ggtcatcaat aaatctcctg
    13201 ctaatctcaa tgcaaaataa ctagatatag ttgcacttaa cattggcaaa gtataaaaaa
    13261 tagctacttc tttaagtata gctctattta tatctttttc tgtagtacct atttttaaca
    13321 aaatcttata tttctcttta tcttcatatg catcactata tattttcata tatattatac
    13381 ttccagctac aattgcaaag actaagaata aaaacacact acatatgcaa acttcatcca
    13441 ttgactctca ccttgagctt tatatccatt ttgaatatac acatttttta aacttaaatt
    13501 cttttcaata ttgttgaaca ttacttgact attttcttct tgaaatacct ttgcaccata
    13561 aaaatacact tgttgaccct tatctcttag tttaaaatat gtatcatcat ttaccactat
    13621 aatactttga ttaagtcctg tccctagtac tggaactttt acttctcccc ttgaaatatt
    13681 atataactga ccatcaagtt ttatcttggc ttcttttgta gctattgatg ctatcgtttg
    13741 agggcttttt aactctatta cattatttcc actaactaat ctttcaccat actcttttaa
    13801 ttctttatca ttgccattca ctttaagagt attacaaaaa tcactatatt tcattatagt
    13861 atctgttgta tcataagctt cttttatatc tgttttcttt aaaattgtta gttctggatt
    13921 tactgagtac tttatcttat tagtatcttt tattatattc tctatttgtt ttttattcac
    13981 ttcatcttta tccattgaaa ctatgctaat agtgtacaca tttctttgtt cttgggcatt
    14041 cttatgtata cgcttcatcg caactgaagc accaagagtt gatattgttg tagttataat
    14101 tatagctatt atagcataag ttttatagtt tttatttaat cggtagttta tattgtttat
    14161 tgcaattata ttatttccat taaataatat ttttttattt ttgattataa tatcaaatat
    14221 aacaggcata actgatttga ataatccata agtacctact attattatac ctataacagg
    14281 tagtacacta gttatatctc cagttcttat attggtagaa agatagtagc catatcctat
    14341 tactataatt gatactatag ctaatatata tatacctatt cctacctttg gtattttttc
    14401 ttgtttttta gatatattga ttagatttat tacactactt ctacatattg ttataaaacc
    14461 ttttacagcc attattataa atatagaaat aaagattaaa gtagcatata gtatagcttc
    14521 tatactaagt ccattagata cctttatatt aaatccagat aactttatta tcactacttg
    14581 aaaaaatcta gatgttatta tccctataat tattcctgtt atacaagaac ttattcctat
    14641 aagcatattt tctataaaat atatttttcc tatagtatat aaatctaatc ccataaaaga
    14701 caatattcca atttctttag acctattctt aaaaaatata tttgttgcat accatataaa
    14761 gaaaaataaa aatactgata gtatcattat tacagctact aacactgata gtgtaaattt
    14821 tttattttca tcttgtaaca catttatagc atcaccatat cttaataatt caaaattagt
    14881 tagcacaact actgaaaaaa ccatagatat aaaaaatgct agataagttc ttatgttact
    14941 cttaaaatta ttataagcaa ttccaagtat actcacttac atctcacctc ccatagaggc
    15001 tagcatatcc ataatttttc cataaaattc ttttctacta tttcctttgt ttaatttaca
    15061 ctttatattt ccatcattta tcatatatac ttcatttgaa taacttgctg ttagagggtc
    15121 atgtgtaatc attataatag tagcgttatt ttctctgttt attttttcta atgattctaa
    15181 aagttcatat gctgatttag agtctaaagc accagttggc tcatcagcaa atatgactgt
    15241 tggatttgta attaatgacc tagctgctgc gcctctttgt ttttgtcctc ctgataactg
    15301 atatggatat ttatctaaat gattttctaa tccaaatttt ttagctatag aaaatacctt
    15361 agcttctatc tccttacttt tcttttttcc taatgctaga ggaagtgcta tattgtccat
    15421 tagtttcata ttgtctaata gattatagtc ttgaaatata aaccctattt tttctcttct
    15481 taattttgat agttcattat tttttacttt tgatatatta attccatcta gtattatttc
    15541 tcctgaagtt tgtttgtcaa gtgtagacat taaatttagc aatgttgtct ttcctgcacc
    15601 agatggaccc attattgata taaaatcacc ttcattgact gtaagagata tattttttaa
    15661 cacatttgtt ttaaatcctt ttattccata atcttttgat atatttttta tttttaatat
    15721 ttctttcata ataatagcct ccagttgcta attttttaat atatatttat tttaaaggtt
    15781 ttatataaat aaatccttaa gtaaatatta cattttaact gttagcatta cacttttgta
    15841 acatatggaa gactattata attatagtag aaatatctta aaataaaaac ttgcttcact
    15901 tttttgtata aacaaagaaa agaagctcct aagaagcctc taatctatac aataattctt
    15961 ctctataaaa aaccaaaata cacattgatt gtggcaaata cagatactat aaacaagtga
    16021 tattactact atatattaaa acaattattc ctgaagataa ttcaataaga ctatcaagca
    16081 ataagctaag catccaaaaa gacttaatac cattaatgta taattatacc caatattaat
    16141 gctcaaaaaa ccagcaacag ttggaataat cagaaatgaa cctcccataa atatacttaa
    16201 agctattaat tggctactac ttctaagttt tttaggagaa acttcaaata ttaatctttt
    16261 tgaagtaatt aaaattaaag gagtagaaaa cacttgaaaa acacacagta taataatgat
    16321 atttgaatca tttgttaaag caaataatag aaactgaact gttatcataa tagcacttaa
    16381 ttttaataag tttaaactgc tatatttttt taataaataa gaaccagcca gatatgttgg
    16441 tatctctaac aatgactgta aagaccactt taaagatatc tgagaatttg tagcatttaa
    16501 agctatcatt ttatctatta cagtacagtt atttgctacc accattgagt ataataagaa
    16561 taaaatacat actagtaaga tatatttttt atcttttaat aattcaataa tatctttatt
    16621 agttacattt attttcttat taatcttttc aatatcaggt aataatagta tatttataat
    16681 aagtaatact acaattaaac caatcgctac agataatcca aaatatgaga atctaagaat
    16741 caagtaagat gctaccatac ttccaatagc ccatccaatc gacccaaaag tcttaataaa
    16801 tgacaaataa ttattaatat tatctccaca acctattatc catgtatcat ataaactaca
    16861 gcaagtattt aataatcctc ctgataaagc aacaagtaat aagtatatta taaaagttgc
    16921 agattcagta gaaaacataa taactgatgc aattccataa actgataaac taattaaaac
    16981 aatttttttc attgtttgat acttatcagc taatactcca aataataatt gaaacaatat
    17041 attggttatt gcatagctcg ataatataat tcctctttca aacgctgagt aacctaattt
    17101 tgataagaaa ggtgtgaatt ttgttgaagc aaaggcaaaa ataatatatg agaaaaaata
    17161 gaatatgata aatttaaatt taatcgcttt attaatcata atcaatcaaa attgataact
    17221 ttaataccac tatttccttc ctgatttaag tttgaaagat gatattttac cgtattagga
    17281 ttctctacaa taactaaagg aatatataaa gtattttcaa gtaacttatt atcaatttct
    17341 actaactttt cccccatatt aacttcttga ccttgtttaa cttctaattt aaagttttct
    17401 ttttttgttg ttgcactatc caatccaata tgaataagaa tttgaacatc attttgcaat
    17461 ttaattccta tagcatggtt agtttgagca ataaaactta ttactccttt agtcggtgat
    17521 ttaagaatat tgtcctttgc tatcatagca aatccatctc ccattacctt agttgaaaat
    17581 actttatcag gaatttctcc aagattaatt atttttccat caatgtatga attaattgta
    17641 attgcttctt tttttctttt aaagaacatt atagacactc ctctacataa ttaactttat
    17701 tctaattatc attacaaata acgatattaa gcatactgca attgaggaaa aaagagcctt
    17761 atagctcttt ccccattgta gttctttaat actgcttatg atttgtttaa ttccaacaaa
    17821 tttataaata taggtaaaca tgataacaat agttattgtg taaatttgga atatctgccc
    17881 taataagact aaatagaaag caatcatgtt ttcttttatc tctttattct tttttatatt
    17941 gtaaataaca tatgcactgt atactgcact aaagttagag attaaattta aacttgcatt
    18001 aacatcattg tttaaaagtt gtgaaaatga aatatttgta aataatgatg tcaattctaa
    18061 atacactata tataaaacag gtaatattag aaaacaaacc ataaccactg aaagaaatat
    18121 attttcatct tttaatactg ctgtgatatt ttttatttta tttgttatca tgtttttgtt
    18181 ctccttattt aaaagttggc cagaaatcct tattagccac aatcatttca tctaagattt
    18241 cttttgctac ttttgcactt ggaacagttt tactcatagt aaacgactgc cataattttt
    18301 gataactttt ttctatccat gcttctacag ctaatttttc agaagcaact tgttgttcca
    18361 ttaaaccttt ttggaaagtt ggaattttac ctatagacat aggttcaacc atatcttttc
    18421 caacaaggca aggaatttca accattgcat catctgcaaa attttcaata gctccattat
    18481 taggaacaat cagtaaaaat ctttcctgag tattaaatgc taatgcacaa gctaattcaa
    18541 caataaattg agaatgctca cttgcatcaa accatgtatc ccttgctgtg ccatttttta
    18601 taattctttc acattcttcg aatgtatcct tttctcttct atccataatt tcatttgctc
    18661 ttgtataatt tggattagca tgttcaacag tttcatcagc cattaaataa tattttaaat
    18721 aagtattagg taatgtagtc ggttctattt ccaataaatc ctttgctgct ctcatagttt
    18781 ctaaccaact agattctttg tgttgacctt gaggtgtaaa ttcctcatat ccttttttag
    18841 cacaatattc ttgtaattta ggcattaaat catttccttc tttgtcctta atacttgtcc
    18901 accacccgaa atgattcaat ccataatatc taacatcaaa gtctttacga gaatttaaac
    18961 ctaaaactct agcaaatatt tcctctaaac atactggcat atcacaaata ttaataattc
    19021 ttgaatttgg tcttaataca cgacatgctt ctgcaactat agctgctgga tttgaataat
    19081 taagcatcca agcatttgga gaatattttt ccatataatc aatcatttcg attattggac
    19141 ctattgttct taatccatag gcaattccac ctgccccaca agtttcttga ccaacaaccc
    19201 catattttag tggaattttt tcatcttgtt cacgtaaagc atataaacca acacgaattt
    19261 gtgccattac aaaatcaata tcagtaaatg cttcttctgg atttgttgta taactaaatt
    19321 taatttctgg atttttctct ctaacaatta ttgcacatgc ttcagcaact tttctttgtc
    19381 tctcatcatt attatcatat aatttaattg attcaatagg aaatttagca acactttcaa
    19441 ttaacattgt tacaattcca gcagtataag tactaccccc accagcaatt agtatcctct
    19501 gtctttttct ttccatcttt ccttctcctt tataataaat tttcaaattc ttctcttaca
    19561 cttggtactg aaagcccaat aataatctga atagcttttc cattatgcac taaaccatgt
    19621 gcacctactg ccttaaaatc ttcttcttta gaaataattg agtcatcttt caaggttaat
    19681 ctcaatctcg tagcacagtt tgtaacatct acaacatttt ctgctccacc aactaatttt
    19741 atgtattcat atgcttgttg agaggcttta ctcaatttat ttcctttttc atctttcgtt
    19801 tctaaatatt catttttagt atatagtttt gtttcttcac tatcttcttc tctaccagga
    19861 gttttcatat caaacttctt aattaagaaa gtaaatacaa agaaccagat aaatgaaaat
    19921 ataatcccta caacaacttg aacaacatat accttccagt gatacattcc aagaggaagc
    19981 caatttaata caacccagtt aataattcct ccaccaaaat caccaacaac tcctaaggca
    20041 aacgaaatcg tttgcaagca tgcatctaaa aatgcatgta ctaggaataa aagtggtgct
    20101 acaaataaaa atgtaaattc taacggctca gtaattccag ttaatactgc agtaagagta
    20161 gcaggaatca ttaaacctaa tacttttttc tttttttctg gctttgcagt tacatagaaa
    20221 gcaccaaata cacctaatga accaaacacc ttcgataatc cggataatga aaatccgatt
    20281 ggatacattt cctttagagt cttagctgaa gtttgaaaat cctgaatatg agtagcccaa
    20341 tatactgatg taccacctgg aacaacaaca ctatcataac aaattggtgc atatacaaaa
    20401 tgatgtaatc cagttggtat taaaatcttt tgtaagaatg cataacacca aactcctata
    20461 tttccactat ttataaaaaa cgtttgcaaa tgtctaataa cattttgaat atgaggccaa
    20521 ataaaacaag ttaataatgc aactggtatc ataacaaaga aacaaattgc aactacaagt
    20581 gatgagcctc taaataatcc aataaaatct ggcagttctt tatcataaaa tctattatga
    20641 atataaatga caatacctga tattaacaaa gcaccaacca taccagtatc taatgtttta
    20701 ataccagcaa tgcttacaag tccacttcca gcacttattt cagctgcgaa atcaactcca
    20761 aagctttttc cccaaaaact taacatagaa cctacaaaat aattaaaagt caaatacgtt
    20821 gctaaagcct ccatacaagc tcttgcttgt tgcttttttg caagtgctat tggtaaagaa
    20881 atagcaaata ataatggcaa ttgattaaat accatccaag ctccactaga tattacaccc
    20941 caaaattggt accaaatatt ttctggtgta gcaggtgaac ccataattga ctgattttga
    21001 aatacaattg agaatccaac cattatccca gcaaaagcaa ataatagtgt tggtgtaaac
    21061 attgcccctc caaaacgctg tattttcttc atcatataaa ttactcccct cctcatattt
    21121 ttagtttatc tttggcctta gatatctatt attttaacag gttaataaat atatacaatc
    21181 tttagggtac attcggagct tattcacagt caaataagtt tttgcacatt tttatgttca
    21241 aattctcata aaaaaaaaga atgactacaa tttgcaatca atcccttctg taatcattct
    21301 atgcctttta taaatactat atttaataaa taataactca atcaatataa aatataacat
    21361 tgtcaattta taactagggt gcatttgtaa aaatgagata ttagtcgctg aaatataaat
    21421 tgattcatca cttaaatcag tcaatgtatt atttttaaac tctgtaattg aaattatttt
    21481 agttcctatt aattttaatc ttctagcaat attaagtgta ttctcattct caccacttag
    21541 ggatataata atcataacat catttttact gacaatattg taaaaagaat catcaaatgt
    21601 tcttccctca aaatcatata ttatttcttg acatgataag aacattctct ttatctgttt
    21661 tgcaaccaca ttttgaatat cacctgaacc ataagcgtat attgttcttg attcataaag
    21721 aagtttacac aatgcatcat aatctttatt tttttgatct tcaacatact taattattga
    21781 attacatgca atatcaaatg actgattttc tatgacatga accttttctg attcccagct
    21841 taataatact ttaaactcac tgaaaccttt taaatctaat tttttaacaa atcttgtaat
    21901 agttgcacga gacacattac atttcatagc aagttcattt attgacatct tactacaatg
    21961 ttttttattt gcagttatat atttccatat taataaatct gtatcattca atttattata
    22021 atttaaaaaa atcatttctt ctaatctcat aaactattac cccctttaat taaaaatcgt
    22081 cataaatatt ataccaatat tcaacattaa attacagcgt attcttatat ttactattta
    22141 gcccataaaa ttgactataa aaaatactgc tataattgat gtttaaaaaa atgtagtttt
    22201 aattttattc ttttatctaa taattaatat tttcaagttc tagtgctttt cttatacaat
    22261 tatcatcttt aaaatcagca gtccttttac aatcttttac tttaaattct ggaatagttt
    22321 taaattcctc atcacatatt ccattcttat ttaaatacat acaacttgcc attctaacta
    22381 ttagaccact attttttaaa ttaaacaata cagggtctat acaccctcca tctccgccag
    22441 tagtttcacc aactaaagtc gcaaatttat tttctttaca aaacattgca aatgactctg
    22501 atgatgaata tatttttcta tcaacaagta agtatatatt acccttaaat tttgaatcat
    22561 ctgtagattt tatagtatag gaagtatctt caaaatcatt aaaatctttt ataatttctt
    22621 taggtgcatt ttcctttaca cttgctggta aattttctac aggatttaat tttatatttc
    22681 ttgcatttgt atattttttt actatttcat tattatttct ataaattcta taaccattta
    22741 ttttgacatc attttttatg agtttagata cgattccctg ccaataccta tcacttccac
    22801 ctaaattacc tcttatatca ataattaaag ctttatgttt ttctaaagtg ttaatataat
    22861 ctccaattat ttttaaatca ttatttatag agccattctt tgatgacatt gatggtaaat
    22921 acatatatcc aatctgaccc ttaataacat cctttaaaat taattctttt tttacaaaaa
    22981 tatcatctgg catttttatt ttaagattca tagagtcata tctatccaca acttttttat
    23041 cattaagaaa atcataccaa tcattattag agtatgattt cttaaaaagt tcatatcttt
    23101 tcttattatc tattacttct gtgtgtttat tatttaaatc agataatata gatgacagtt
    23161 cttctataaa ttcttcatca ttttcagtgt tttttattcg ttttgtatat tcatccttat
    23221 ttgctaacca atcaatatta ttaagcctct tatttacacc tagatatgga tatcctgtct
    23281 ttacaacatt atacacataa ttaaagtcat ccaccttttc ctcttcagac aactgattct
    23341 tttgagaata agtagaatta cctaataata ttataaatgc taaaataatt actaaaaata
    23401 atactttctt tttcatgtaa ttctcatcca ctttcagatt ttatatttta atacattgta
    23461 tcattatttt attatcacct aaacagttaa aagaaattct tgaagatata ttccactatt
    23521 attctattta aaaattttta tttgtatttt caaaattatt tcatgaaagt tataaaataa
    23581 caattatatt caaatatcta ttttactgct atatcctgaa agtaaattct tatcagtgat
    23641 atactaaaaa agatatgcta aaaaatttat ccattataat gacttaaatt tagatacttt
    23701 actgattttt atttattagt tgagaaagat attgataatt aaaaatattg ataactatag
    23761 aaatgattat tttttataga aattttaagg atataaacta attatactta agtctatatc
    23821 cttaatttat catgattatg agaattttta caaatactta tctttgtccg ttttagaata
    23881 aaaacaagaa catatcctgt tattttattc aaacatccaa gtttaattac tacacaatta
    23941 cttattagaa attgtatcta cttcatatat atatctgtta agtatagctt ctagcatttc
    24001 ttgtcttcca gacttattct ttataactgg catttgcagt gcataagatt ctaactcttt
    24061 aaatccaacc ttaccttcta ctatatcctt acctatacct tcactaaaac ttgcatatct
    24121 ttctttagta aagttctcaa atacctcatc ttctaataat ttatgagcta ttaataatcc
    24181 ctttgcaaat gtatccattc ctgcaatata agctaggaat aagtcttcta cctcaaaaga
    24241 tgctcttctt actttagagt caaagttaaa tcctcctggt gctattccac cttgctttaa
    24301 cacttcatac atagcaagtg ttgcatcata tatatttgtt ggaaattggt ctgtatccca
    24361 acctaataat aggtctcctt gatttgcatc tatactacct aaaacattgt ttattcttgc
    24421 tatatttaat tcatgttgga aagtatgtcc tgcaagtgtt gcatggtttg cttctatatt
    24481 cattttgaag tatttatcca gattatactt tcttaaaaat cctaaaacag tagcagtatc
    24541 aaaatcgtat tgatgtttag taggctcttt tggcttaggc tctattaaga attgtccact
    24601 gaatcctatt tccttagcat aatctacagc catttgtaag aatcttgcaa agttatccat
    24661 ttcaagttct gtattagtgt taagtaaagt ttcataacct tctcttccac cccagaatac
    24721 aaaattttct cctcctaagt acttagttat ttccatagct tttttaacct gagctgctgc
    24781 atatgcatat acatctgcat tacatgttgt agatgcacca tgaacaaatc ttggatttcc
    24841 aaacatattg gcagttcccc ataataactt tatacctgtc ttttccatat taacttttat
    24901 atactcaact atttcatcta atatcttatt tgtctcttct aaatctcttc cttctggtgc
    24961 tatatctctg tcatgaaaac agaaatactc aattcccatc ttagacataa attcaaatcc
    25021 tgcatcaact ttaatctttg ctatctcaat tgggtctgtt atattattcc aaggtctctc
    25081 tactgtccct actccaaatg ggtcattacc ctctccacat aaagtatgcc aatagctcat
    25141 tgcaaatctt aagtgttctt tcatagtctt attacctacc acttgttcag gattgtagta
    25201 tctaaatgct aattcattgt cagattttac tccttcaaat tttatctgtc ctattccttt
    25261 aaatatttca ctcatttcat gtcctcctaa tattttacac atataatata tgtagtatgt
    25321 tttcttaatt taaaactatt ttctatcttt atttttcttt ttaagctaat ttaattattt
    25381 atttttgcat taactacttc atctaataac ttaaagtcat ctttcaaaga tttgtatatt
    25441 cttttataca atctatgata tatattgtat gtatctacat tttcacttat tggttctaat
    25501 ttagttgaag tttttattat agcatcacaa gctttttgta tattttcata tattccagct
    25561 cctactaatg caagaattgc aactccaagt gctggtcctt cacttgaatt tatagtatat
    25621 acatcttgat taaacatatc tgcttgcatc tgtctccata ctttactttt tccacctcca
    25681 cctgaggctc ttacttcatt aacgtctata ttaagattct ctattatatc catacagtct
    25741 ttaagactat atgaaacacc ttccattatt gaccttgcga tttctttttt agaatggatt
    25801 ggagataatc caaagaacac acctcttgca taagggtcaa gatgaggagt tctttctccc
    25861 atcatatatg gtaagtaaag caatccatta catcctgttg gtactttact tacttgatta
    25921 tttataatct catatacatc ttcacctaaa gaatttgaaa cttccacttc actttgacag
    25981 aaattatctt taaaccattt taatgacaag ccagctcctt gagtaactcc cataacatgc
    26041 catgtatttg gtattgcatg acagaaagta tgaactctac cttctttatc aatttttggc
    26101 tcttttgtat aagcaaatac tacacctgat gtacctatag tagatgaaac tactccatct
    26161 ttaactattc catttcctat agctcctgct gcttggtctc cagctcctcc taccactatt
    26221 gttccttctt taagccctgt caaacttgct acatctctag tcacataacc tgtaatttct
    26281 tgtgattcat aaactttacc taaaagattt ttatctatat ttaacttttc tagtacttca
    26341 tcgctccaat ctctttttct tatatcgagc aactgcatac cacttgcatc tgatacctct
    26401 gtagcatatt cacctgtcaa tttgtatctt atataatctt ttggaagaag tattttatta
    26461 gtatttttat atatatctgg ttcattgttt ctaacccata atatctttga tgctgtaaat
    26521 cctgtaagtg ctggattagc agttatttca atcagtcttt cttcacctac taaatcagtt
    26581 atctcctcac actcttttac tgttcgttgg tcacaccata ttatagagtt tctcaatgtt
    26641 ttaccttcat tgtccagaat aactagacca tgcatctgtc ctgataatcc aattcctttt
    26701 atacattcac tgcttatatt tgaactttga actacttctc ttatagattc gactacagct
    26761 ttccaccaat catttgcatc ctgctctgcc caacctgatt tctcttgtat aagaggatat
    26821 tcatatgtac aactttttat agtatttcct agtttatcaa ataaaactgt tttcgtacct
    26881 gatgtaccaa tatccacacc taatacatac tccaacttca tccctccttg aatgccactt
    26941 cataactatc gttttcctat cctaattttt ttatatttta ggtatatttt acttgcttta
    27001 tttttgttag tatattaaac taacttatga actaataata aaatatttaa ttatctttgt
    27061 caatgcaaaa taacctttta tttttgtgta aaaagtatta caatattatt ttaaggagga
    27121 atatcaaatt ggttactgat aaatacacta ttcgagaaat gaatgaaaga ttggtacttg
    27181 aacaaattat aaaaaatggt cctatttcta gggcaagtat agcttctacc ataggattga
    27241 ataaagcaac tatatccgct attaccaaaa aacttattga tgaatctcta gttcatgaaa
    27301 tcggaattgg aaatagtact catagtggcg gaagaaaacc catattatta gtatttaata
    27361 aatgtgcagg gatttcttta agtatggaca ttggatacga ctatatattt tcttctctat
    27421 cttacttaga tggaactatt ataaattcta agaaattaac agatatacaa gtaagtaaag
    27481 acaatgttat acaattgata gatgaaatta taaatagtta taacatatcc aaaatagaca
    27541 ctccttataa ggtaattggt cttactttgg ctatacatgg gattacttgt gaaaataaag
    27601 tattgtttac tccatattac aatcttaatg agattgactt atatagcatc cttagcaaaa
    27661 agtatgactt tcctattcac atagaaaatg aagcaaactt aacagcttta gctgagaata
    27721 ctttttcaac tgtacataac tcactactta gtctcagtat tcacagtgga tttggttcag
    27781 gcattataat aaataataaa ttatatagtg gcagaaatgg tatgagtgga gaaattggtc
    27841 acactatcat catgcctaac ggtaaactat gtccttgtgg aaatcgtggc tgtttagaac
    27901 agtattgttc agaaaaaaag gtatttgaac agctttcttc tttagaaaat attcctaaaa
    27961 tagattctga tatagtaaag caactatatt atgaagataa ccagaatgcc aaaaaagtta
    28021 ttcatgagtt ttgtagttac ttaacaatag ctataaataa tgctattaca acttatgctc
    28081 ctgaaataat atatttaaat agccaaataa ttagtgatat ccctgagata ttacagatta
    28141 caaaagatat gttagttagc tcttttaaca aaggaataaa tatagaaatt tcatcattag
    28201 ggtctgaggc atctctatat ggtggctctg ccgtaaatat caagagcttt ttgaatatac
    28261 aaaatttaac tttaattaat gaaatataag ggttatgact atatacagcc ataacccttt
    28321 aaattataca ttctaaaatt agatttatta tatagtttat gcaagattag ctatattttt
    28381 taaaaattgt atatcaatta aatattaaca taagtatttc gttttaaata ttaatataat
    28441 cttcctactc atcatcagat aataaacttg cattttgttc aaccatagaa atattatctt
    28501 tacctatttt ctctaatttt gctgccaatt cctttgtatc taaaaaagct gattcaataa
    28561 ccatacctaa atttagccct gaaataattt taattcgact gtcattattg gctactaata
    28621 aagcagcatt gcatggagtt ccacctagta aatctgccat tataattatt tctgaacttt
    28681 ccttgtacac ttctaaagct ttttttaatt tttctattgt tccttctatt ccatcatctt
    28741 gtttcatgtt tacagtagga taatctatag aatcccctat tatcattttt gcagaattta
    28801 agacttcttc agccatattg ccatgactca tcaaaataat actattcaat ttaatacacc
    28861 tctttaaatc tataagatgt ctacaagata agttttctta tcatcaggcg tcattatagt
    28921 tgtaatttca attccttcat ctaaaagtct tttaaatgct tctttctctt cttcagtaac
    28981 actgatatta ggtcttagaa ctgttgtatc ttttctactt gacatatttc ctacatttat
    29041 attatgtatt ttaaatcctg cctctacaaa tggaataagg tccacaggag acttaacaat
    29101 taataataca ttttgtcctt catactttcc tgcagtaata ttttttatag cagtctcttc
    29161 tgtaattata gatgacgcta tacctgcagg tgtagccatt cttaaaactt gtttttgaac
    29221 agaattgtta gctactgcat catttactac cattaatctt gtaacaccca atttattact
    29281 ccacatagtt gcaacttgac catggattaa acggtcatct atacgaatat ttttaatacc
    29341 tttcatttcc atatctaata tcatcctttc cttattttaa aatacctaat gcatataaaa
    29401 ttattgaaac aaccattact cctaaaatta agtatatagg ttttatattc tttttaagag
    29461 cccaataaac aatcacaact aaaattgcag gaactaaccc aggcataatt tggtctaata
    29521 tttcttgacc tttcattgta aaatcacctt gttgaaagtt caaagcaaca ttagctttta
    29581 caactgaagg tattaatgca cctacaacca ttaaacctaa aatagatgta ctctcagtta
    29641 aaatctttaa acttttttca attgaagata ttagcttagt acctgatgta taaccagcat
    29701 taaatagtgg tatacttaat aatttcaatg ctacactcac tacaatccac agtaagattc
    29761 caaatggact tccatctatc gccatattag ctgctatagc tccaaaaatt gtccacggaa
    29821 taacgacaaa taaactatct cctaaacctg ctactggtcc catcaagcct gttttaattg
    29881 aagcaacagc ctcttcagtc tcttcacttg ataactcctc cattgctaca ttaataccca
    29941 aaatataagg ggcaacccaa ggattagtgt taaaaaattg taaatgattt aatacagcac
    30001 tttgtaactt ttctggatta tctttgtata ctacttttaa aaaaggtagt actgcataac
    30061 agtatgccaa ggcctgcatc ttctcataat ttgaagaaac agaactagta aaaaaccagc
    30121 gccataagac actttttttc accttttcat ttgatttatt taatgatacc ttatcactca
    30181 tcttcgtcca tacctcctac tacagtatat tgttgttctt gattttccaa attctttttg
    30241 taaattataa gtgcaattgc tccaccaatt aaagctacac ctaataatgg tactttcata
    30301 tatactgcta aaacaaaacc aattactaag taactaaaat actccttagc aggaagatat
    30361 ctaagtaata atcctatccc tactgctgga agtagtcctc cagctactgt taaaccacct
    30421 gttaaccatt caggtgtata tttcaaaatg gtattaatta aagaaggtcc aaatattaca
    30481 gatagagcaa ctggaatacc tgttgtagct gctgttaata gtactcctaa catttgcata
    30541 taggttatat ttttataatt tcctttttta gctccatctt ctgctttatg aactagccat
    30601 ataccaatag tatttctaag aacatctaat tgtaccatca ataaagcaac tggaatacct
    30661 atagagattc ctacagatgc ttcttgctta gtcgtaatag ttataaatgt ggctactata
    30721 cttgctgttt gataatcagg tattgatgct ccaccaaagt tactaatacc taatgataat
    30781 agctgtaatg tacctccaat atataaccct gtatttatat ctcctaagat aagaccagta
    30841 ataaaacctg ctgttactgg ttgatatgtt ccaaacattg ttgaattttt ttcatagttt
    30901 ataaaaaatc catatagtac gattaataat aattgccata attgtggctc cattatgatt
    30961 tcacctctcc caaaatattt tttagttctt taaaattata actggaatct tgctttagga
    31021 aaataggaat ttcttccagt gggcattcaa cttcataagt tttcccacct tcataactaa
    31081 ctccactaaa tatattttcc catgtatgtc cttttggtaa atatactttt ctacttctat
    31141 ctttataatt cattattgga gctactaaaa tttcatctcc aagcatatat gtattatcca
    31201 cctcccaagt tttatcatct tcaggaaatt cataaaataa agttctcata actggtgttc
    31261 catcttcatg cgcttctttc atcagttttt ttaaataagt ttttaagctc tctctaattt
    31321 ttatgaattt tgtaagaatt acttcaactt cttcaccata agaccatatt tcattaggag
    31381 ctccagttac cattgagcct ccacctttat ttgataatgg tttactatga ggcaatctat
    31441 ctccatgcat tctcaaaatt ggtgaaaaag tagcatattg gaaccatctt accataagtt
    31501 ctctaaattc tgggtcttta ggatttcctc catggaaacc tccaatatca gtagtccacc
    31561 atggtatacc tgccagtccc atatttaacc ctgtatttac ttgattatta aaagcctcaa
    31621 aactagaatc aatgtcacca gaccatacta aagctccata cttttgagct cctgcccaag
    31681 ctcctcttac aagagttaca acagactcta tgtcattatg taaaccatca taaatcattt
    31741 ttaaataatc aataggataa atattaccac aacttaaaac atttcctttt ttatatctat
    31801 agttatcaaa atcatacaca gaatagccag gttcagctac atctaaccaa taatagtcaa
    31861 tcccaagttg tttataattt ttatctattc tatcccaaac atattctctt gcattttcat
    31921 ttgtcatatc tacaaagact gtatttcctt gaatttgcat tgtcattcta acaccacgat
    31981 taacattgac taaatagcca ttttctaaat aatcattgta gttttctgca tcactttgaa
    32041 ctgtaggcca tacagatact attggttcta ctgaatactc ttctttcaat tttttaacta
    32101 attctttagg atttttccaa taatctaagt caaatttata ttcaccttgc ttaggccaat
    32161 gaaaatagtc aattgcaatt gatgaaagct taattcctct tttagaatat tccttcacaa
    32221 catctaaaac ttcttctgaa gttctatatc ttagtttact ttgccatagt cctaaaaggt
    32281 tttcaggcat cataggaaca gttccagtaa cttgagaata atttttattt aactcttttg
    32341 ggctttctcc acaagtaatc caataatcta taaaatttgt agaaaacatc ttccattctg
    32401 tcatattttt agcaaaagat acttttccaa ttcccggatt attccataaa agtccataac
    32461 ctagactaga aatataaaaa ggaactgata tttgagaatt tctttgggct aactccaaaa
    32521 cagtattctt taaatctaaa aatttatgtt gatattgtcc cattccaaaa atcttttcag
    32581 aaggctcaga ttcaaatcta gtagttactt caaattctcc actatagctt ggtcgatatt
    32641 ctcttgattt taactttaga gtagagttaa agtctttggt aatctcaatt gtccctacat
    32701 cttctccacc atcatcatgt tttacagctc ttaatcgaat atattctttc aataatattt
    32761 catttttatc attataaaaa gtaattctat ctctatggtc taagacagct tttatctttc
    32821 catttttaat gattgcactt ccatcttcat ttttattaat tgtaatatca tctttattta
    32881 acttaggttt ttcgttaaga gcatagtttt catcaacaaa attttggtct acaaacgacc
    32941 taatacgtaa agagtttttt ccccatgctt ctattcgcaa tagttcattg tcaaatcttt
    33001 tttcaatata gtcttcagat atttttatca cggctacctc ctaaatcaat ggtataatat
    33061 tctatctttg tgtagcttaa gttaaataat gtcgtttagt atcgttattt ataaattagg
    33121 ttttatatat aattcatttg tacattaaac taacttacca ataatatact ttatttgact
    33181 ttgtttgtca atcgctttcc ggattttagt aaaattttct aatatgttgc ttgaaagtcg
    33241 ttcttaatat cttttattta tacttttaat atctgtgatg atacctatat acatctagtt
    33301 tttttaaaac acatacagaa ataaagtttg cctattatgt agataatatc ggacttgaaa
    33361 atatgtatag tgacct
//
LOCUS       DAEOSP010000024        31477 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.024, whole genome shotgun sequence.
ACCESSION   DAEOSP010000024 DAEOSP010000000
VERSION     DAEOSP010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31477
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.024"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            161..835
                     /locus_tag="KRH54_000894"
     CDS             161..835
                     /locus_tag="KRH54_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8578563.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNSYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            823..1983
                     /locus_tag="KRH54_000895"
     CDS             823..1983
                     /locus_tag="KRH54_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG8578564.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSDHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            2254..2727
                     /locus_tag="KRH54_000896"
     CDS             2254..2727
                     /locus_tag="KRH54_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBG8578565.1"
                     /translation="MLSSTFCSLPFWICYFWCGISDIFDGLIARKLRQQSGIGAKLDS
                     IADFIFSVTILVTVGKNIALPMYLWWCIVAIALLRLISYSIGFFKYRTFSSLHTLMNK
                     ITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIMIKSKELNRDIKSIFM"
     gene            3013..3681
                     /locus_tag="KRH54_000897"
     CDS             3013..3681
                     /locus_tag="KRH54_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8578566.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKEYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            3684..6152
                     /locus_tag="KRH54_000898"
     CDS             3684..6152
                     /locus_tag="KRH54_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8578567.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITLGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGNLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSFISALPSAIYASHVSPKIAMVGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKITKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(6392..7816)
                     /locus_tag="KRH54_000899"
     CDS             complement(6392..7816)
                     /locus_tag="KRH54_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8578568.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(7835..8527)
                     /locus_tag="KRH54_000900"
     CDS             complement(7835..8527)
                     /locus_tag="KRH54_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8578569.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(8582..9358)
                     /locus_tag="KRH54_000901"
     CDS             complement(8582..9358)
                     /locus_tag="KRH54_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG8578570.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            complement(9355..10125)
                     /locus_tag="KRH54_000902"
     CDS             complement(9355..10125)
                     /locus_tag="KRH54_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG8578571.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(10122..10826)
                     /locus_tag="KRH54_000903"
     CDS             complement(10122..10826)
                     /locus_tag="KRH54_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG8578572.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(11081..11593)
                     /locus_tag="KRH54_000904"
     CDS             complement(11081..11593)
                     /locus_tag="KRH54_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578573.1"
                     /translation="MKKISQEKISTHIQEIPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSGCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            11919..12176
                     /locus_tag="KRH54_000905"
     CDS             11919..12176
                     /locus_tag="KRH54_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578574.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            12319..12945
                     /locus_tag="KRH54_000906"
     CDS             12319..12945
                     /locus_tag="KRH54_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HBG8578575.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(13192..13971)
                     /locus_tag="KRH54_000907"
     CDS             complement(13192..13971)
                     /locus_tag="KRH54_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG8578576.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(14039..14500)
                     /locus_tag="KRH54_000908"
     CDS             complement(14039..14500)
                     /locus_tag="KRH54_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8578577.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(14476..15153)
                     /locus_tag="KRH54_000909"
     CDS             complement(14476..15153)
                     /locus_tag="KRH54_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG8578578.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            15386..15772
                     /locus_tag="KRH54_000910"
     CDS             15386..15772
                     /locus_tag="KRH54_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8578579.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVARESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            15775..17574
                     /locus_tag="KRH54_000911"
     CDS             15775..17574
                     /locus_tag="KRH54_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBG8578580.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSPNSVINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDDEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(17929..19803)
                     /locus_tag="KRH54_000912"
     CDS             complement(17929..19803)
                     /locus_tag="KRH54_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG8578581.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLLGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(19808..21472)
                     /locus_tag="KRH54_000913"
     CDS             complement(19808..21472)
                     /locus_tag="KRH54_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBG8578582.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESEDVIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVCLKPFHAVLLKAR"
     gene            complement(21486..22307)
                     /locus_tag="KRH54_000914"
     CDS             complement(21486..22307)
                     /locus_tag="KRH54_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG8578583.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(22406..23170)
                     /locus_tag="KRH54_000915"
     CDS             complement(22406..23170)
                     /locus_tag="KRH54_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG8578584.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(23167..23916)
                     /locus_tag="KRH54_000916"
     CDS             complement(23167..23916)
                     /locus_tag="KRH54_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG8578585.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(24420..25025)
                     /locus_tag="KRH54_000917"
     CDS             complement(24420..25025)
                     /locus_tag="KRH54_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8578586.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGENIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSISELDNLKYEWICMSHGTPCIK"
     gene            25204..25842
                     /locus_tag="KRH54_000918"
     CDS             25204..25842
                     /locus_tag="KRH54_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8578587.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            25974..26636
                     /locus_tag="KRH54_000919"
     CDS             25974..26636
                     /locus_tag="KRH54_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8578588.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            26636..27817
                     /locus_tag="KRH54_000920"
     CDS             26636..27817
                     /locus_tag="KRH54_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8578589.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTIHAENCHNGGARFIIKFY
                     T"
     gene            complement(27998..30658)
                     /locus_tag="KRH54_000921"
     CDS             complement(27998..30658)
                     /locus_tag="KRH54_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8578590.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQDASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(30655..31338)
                     /locus_tag="KRH54_000922"
     CDS             complement(30655..31338)
                     /locus_tag="KRH54_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8578591.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEIASLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
BASE COUNT        11232 a         5024 c         3936 g        11285 t
ORIGIN      
        1 aaaatttcta ataatttcat ccttgattta tttaatatat aaaaatatac taactttaat
       61 gattaagttt tgaaatataa ctaagaattt gataatttat tgagattttt atactaaaat
      121 atacatacta tacaaactta atttagaagg gagcattctt atgaacaata aaatactcgt
      181 cgtagatgat gaggaaggta taataacact cttaaaagat tactttgaaa tgaatagtta
      241 tgaggtttac actgctcaaa gtggaaaaga cgcattaaaa aaaatatcca aaaatccaga
      301 tatcattcta cttgatataa acatgcctga aatagatgga attgaagttt gtaatagaat
      361 tagagattat gtatcatgcc ctattctatt tcttacagct agaattgaaa atactgacaa
      421 gataaatggt tttcgtgctg gtgctgatga ttatattgta aaaccttttg atttagacga
      481 acttggagct agagtatctg cccatttaag acgtgaacaa aggaagtcaa atcaaactaa
      541 aattaaattt tataatgatt taacaataga ttacgaaaaa aggttaattt gtatatataa
      601 taatcagatt tcactttcaa aaaaggaatt tgatatagta gagcttctct ctatgaatcc
      661 tggacaagta tttgataaag aaaaaatata tgatagagtc tggggataca atagcaatgg
      721 aaatagtgat gttataatgg aacacattag aaaaatacga atcaaactat caaaacatac
      781 tcttgaaaat tatatagaaa cagtctgggg agttggttat aaatggataa aataaataat
      841 atgtcattaa aaaaagcttt ctttgttctt actttatctt cactaattat agcatcagtg
      901 cttactactt tcgcatacat tttacttaat aatctatata attctataca agataagtat
      961 ttaaaaacac ctataaatac aggtattata accatagttc aaaacaatgt gaaccaattt
     1021 agtgattatg ataaaacatt acttaatata atcaacatac ttcagcttgt acttccactt
     1081 atatttttta taggactttt attattagct gatataattt tttacaaggt aaaattaaaa
     1141 acacccatag aaatactcaa taaaggtgca atggaaatat ccaacaacaa tttagacttt
     1201 tgcttagagt ataataataa tgatgaatta ggtaatctat gtaatgcttt tgaaaaaatg
     1261 aggtctgaac taaataaaaa caacataaag atgtggacta tgattaatga tagaaaacaa
     1321 cttaatgctg ctttttctca tgatttaaga aatcccctaa cagttctaaa aggttactca
     1381 aactatttaa caaaatacat accaacaggt aagcttagtg atgagaagat tttatcaacc
     1441 acacaactta tgtctgatca tattaacaga attgaatact atgtagaaaa tatgagtaat
     1501 gctcaacgat tagaagattt agttgtttca aaatctatgt cgaatataaa caagtttata
     1561 gaaaacttag atgaaaatat atctatcatt gctaaacaag aaggaaaatc ttttacactt
     1621 aaaagtcaaa taaataatat aaatctattt tttgatgaaa atatcattct tagagtcgtc
     1681 gaaaatataa tatcgaatgc ttttagatat gctagaaata atgtatccat acttatatat
     1741 cttgaacaag aactccttac ctttgtaatt gaagatgatg gtataggatt tagtaaggaa
     1801 tctctaaaat tagctttaag acctttctat agagataaga ctttaaatga cagcaatgca
     1861 cactttggta tgggtcttta tatctcaaaa atattatgcg aaaaacatgg tggctctatt
     1921 tctattgaaa ataattctac taacagtgct aaaattactg ctaaattctc aacaagaaat
     1981 taaaatgatt ttgtaagctt tcactgttct tatataaaat ttgttagttt agaatttata
     2041 aaatgtattt caatttacaa ccaatatata tactattttg tagaagttgt aagagttgct
     2101 tcaattataa tattttggca aaaggcattt gaaacaataa tagaaatata tatttaaata
     2161 tgtagaatag tatgaaatta gaattttgga ggtgattata ttaaaagttt tgctaataat
     2221 attacattct ctcgtattgt gtttgcattg ctaatgctta gttctacatt ctgttcactg
     2281 cctttctgga tatgttattt ttggtgtgga attagtgata tatttgatgg tcttattgcc
     2341 cgaaagttga gacaacaaag tggaataggt gcaaagttag atagtattgc tgatttcatt
     2401 ttttcggtta caattttggt tactgtcgga aaaaatattg ctcttcccat gtatctatgg
     2461 tggtgtattg ttgcaatagc attgttacgg ttgattagtt acagtattgg tttttttaag
     2521 tatcgcacat tttcatcgct tcatactttg atgaataaaa tcactggaat ttctatcttt
     2581 gcgtttccat tactctatat ttgctttggt cttgatgttg caggaattat gatatgtatc
     2641 gtggcttttc tttcttcttt tgaagaaata gcaattatga taaaatcaaa agaattaaat
     2701 cgtgatataa aaagtatatt tatgtgaaaa cttagaattt gtgacaaaaa taatttatat
     2761 aataatgagg aagtaaaaca taaaaaatta tccacaagtt tatgtgtaaa atagttttaa
     2821 agcaatagag ttgtattgta aagcctttag tgcataaatt acattttaaa ttaaaaatga
     2881 tactgaaact gcttatcagc actgcgaatt atccgcaaat agtgaagact ttttagattt
     2941 aagctaaaag ttgggaaaaa attgagtttt aaaacttaca ctcttcttat atagaaaagg
     3001 agagtgtttt ttatgattat aaaagcaaaa caattatcaa aaatatatgg ttctaataac
     3061 aataaagtta tcgcacttaa taatgtaaat ttagaaataa actcaggtga atttgtttct
     3121 gtaattggac cttctggaag tggaaaaagc acacttttac atattttaag tggattagat
     3181 aatcctacct ctggacaagt gttacttgat gataaagata tatataaaca cactgaaaaa
     3241 gaactatctg cacttagaag aaaatccttt ggattcgtct ttcaacaatt taatctttta
     3301 cctgttctga cagcatctga aaatatttca atgcctgtac ttctagataa aaaacaacct
     3361 gataaagaat atttaaatga aatctcatca ttacttggaa ttgcagatag actcaatcat
     3421 cttccccatg aactttcagg tggtcaacaa caaagagttg caattgctag agcacttatt
     3481 gcaaaaccag atataatatt tgctgatgag cctacaggaa acttggacag taaaagtggc
     3541 agtgaagtta tgaatcttct tataaaaact tcaaaacagt ttggaaagac tttagtggta
     3601 attactcatg atgatagaat tgcaaaactt gctgatagaa aaatttcaat tattgatggt
     3661 gttcttatgg aggtgaaata gaaatgaaga attacctttc actttcaata aaagaattaa
     3721 aaagccagaa acttatgact acattcatca ttatagcaat agtactatca actattatga
     3781 caactgtggt tggacagtca attggtatcc tacaaaattt aagaattgaa caagcccgtt
     3841 cttttaacgg tgatagacat gtatcctttc accaacttac taaaaatcaa gttgatgaca
     3901 taaagaaaga tgatagagtt tatcaagctg gtacttcaat aactcttggc agtagtaaaa
     3961 taaaggattc tggtatctct gtacttgtaa aagagtatga taaaacagga ctttccaact
     4021 accccaaatt aatgaaatta aaatctggaa atttgcctaa agataaaaac gaaatagcct
     4081 tagatgaaaa cacactaaag cttatgggta taaaaccaag actaggagtg actataccta
     4141 tgaatttaga tatttcactt ttaaatgata ccataccacc atataattac acagctaact
     4201 ttaaattgag tggcatttta gaggatgatt atacaggata tgtaagtggt atagtaaatg
     4261 gtattgtagg taaaggtaca tcagaaaatc tattacctaa gagatatatt ttatcctctt
     4321 tggattttaa aataaaacag caagaaaagt ttcaagaaat agtaaatcaa ttagctaaaa
     4381 aaataaatct ttctcacaat tcaattcaat ataattggat ttacttaaat gctcttaaga
     4441 tacagtttga aaaagatgaa aatagttcta atagtgatgg tatatctatg attatattgg
     4501 tatctttatt tgttgcctta ttggttttac tggcctctgg acttgtaatc tataatattt
     4561 taaaaatctc tgttacaaag aaaattaaag agtatggatg tttaagagca ataggagctg
     4621 aaccaaacca gatatataag atagttattt tacaaatctt aattttatgt actgttgcca
     4681 tacctattgg agctgtaatt ggaattatat cttctaaagg cataactgga atggtcacta
     4741 atatcttaaa tccagatata cttctagcaa atgataataa agaaattact gagcttatac
     4801 ataaaaatac aacagcatat atgttcccat tagtattaag cactaatgta tctttgattt
     4861 tttcatttat ttcagcatta ccatcagcaa tatatgcttc acatgtctct cccaaaattg
     4921 ctatggttgg tagtactacc aaaatcaaaa gaaaaattaa aagagaaaaa acaataaaaa
     4981 actttgaaag acatctagca tggctaaact taaaaagaaa taaaggaaga actataataa
     5041 ctatactatc attatttatg agtataacag tattcgttgc actgagtgag ttctctaatg
     5101 tattagatgt atctaggtca gtaagtaatt taaaagaagg agatttttcc ttaacaaatg
     5161 aaatatctgg ttttgataaa tcaacactag ataaaataac taaaatgaaa aacgtcaatc
     5221 gcacttcctt tataaagtac tctgaataca aacaggggga aatagataca gatataaatt
     5281 ttgaaaatag tggtgagatg ttaaaaatca ttggaattga tgagcaaact ttaaaagact
     5341 taatgccttc tattacagat tctatactag aagactttaa aaatggttct atctgtttta
     5401 taaaaaatcc gattgctatt tctacaccag gtgtaaaaac tagacataca aatttaaaac
     5461 ctaaagatag tatcacaatc aacaaaaaac aactagatat atatagtaca gtagataaaa
     5521 tgttcttttt acaaggtaat ggctggataa atggtgttga tgttattgta tatgattctg
     5581 tttataacac cttgacaaat aaaaacaagt tcaaccagat aaatatttat gctaaagata
     5641 aatcaaaatt agaacaaata agattgtcta ttgaacagat ttgtgaaaat aatcctggct
     5701 ctcattggat atcatatatt gaatcagata aacaacttaa agagagtttt aaacaaatag
     5761 aatttctagc atgggcagtt atattatttg tgggattgat tggtacttta aacatcatta
     5821 acacaactca tacaaatata aatactagga ctaatgagat tggagttaaa agagctattg
     5881 gtatgagtaa tagtagctta tataaaatgt ttttatggga aggtgtttat tatggaattt
     5941 ttgctgctat atttggttca attgctgggt atgccagtgc tattattata aacatggcaa
     6001 ctatagaaaa gcttgatttc actaatatac caattacttc aattttacaa gctactatta
     6061 tttctgtact tgcatgtatt atagctactc ttattccgtt aagaaaagtt aaaaagatga
     6121 atataattga ctgtatagat atcaatttat aactatttta cagataataa tataaattaa
     6181 attatcaatg actgtctcta ttttctttca aatctaacta ataaatcttt ttaactataa
     6241 tataagcttt aaaaaaggcc tatctcaaaa tagagataag ccttttttca ttgtcgaaat
     6301 aatgaaaata tatttaactt cacaaaaaaa gagtgcctca cgaattaaaa atttcgcttt
     6361 gagacactcc cctttaattt aaaatatata gttaaatgct atgccaaaat ttcacacatg
     6421 ctccacctga ttttgaattt tctattttaa cagaacctcc aagctgttcc actaattgtt
     6481 tgacaatgta aagcccaaga ccagaatgtc caccttttgt ctgcctagct tcatctcctc
     6541 gataaaattt atcaagagcc ttttttaaat ctttagagct aaaccctcta ccagaatcac
     6601 aaatctcata agaaatacaa tttttttcat cctttacagt aatactaata ttaccacctg
     6661 atggagtgta ttgcagactg ttggatatga tattatcaaa aattcgtgtc aacctatcca
     6721 cgtcgatttg aattggggat tgtatattcc cctgcatttt caatataagt tcaacttcct
     6781 tttgatgagc ctgtaattca tagtcttgca ccttttgcct caaaaattca ggtaaattta
     6841 taggaacaag atttaattgt atatttgact tttctaagtc agacgtatat tgcatctttt
     6901 ggacaagagc agcacttttt tctgtatttt ctcttatcac tgtaagatac ctatgaagtt
     6961 tttcattgtc atctgtatta ttaccaataa gtgcatctgt atatcccaaa ataatagaaa
     7021 gaggagattt taaatcatgt gctagagctt ccaccatttc aacacgttcc tgctccatct
     7081 tccactgtgc agataaagag ccttttaatt cgtctttcat ttcagaaaat gcactacaaa
     7141 gtttaccgag ttcattatct gaataatagt cgatttcaaa atcaaggtcc ttttctttta
     7201 tcttcttgga cgcatcaatc aatagatgta atggttgatt gatattcttt acaaagcgct
     7261 tagaaagcca tctagtaaaa cccacaatat atagaaatgg agaaaacaga gccattataa
     7321 taactgcaaa tacaaatctc cctctaatat ttgcaaaggt gattttcact tggtagaata
     7381 gaataacagc tccctcaatc tttccattat cccctttgat tggaacagta tggatataac
     7441 cacctttacg aacagtttta ttgataaaat tattaaataa ttcttctttt gttttaaata
     7501 gttttttggg gttagtacca tataaaatat tgccattatt atccacaact tgatatagca
     7561 tatcatcccc acgaatcgtt ctttttaaac cttcttcatt cgattgtgat aacaaagtta
     7621 tgtttttttc attgatgtat ttttcaattc taggaacttg tcgctcataa tagtttggtg
     7681 gatagatatc cttatttaag gaatgaatat acaaatagag agcaaataca taagtaatca
     7741 ctgtggcaac tatactggct attataataa aaataaaagt atgtctaaat tgggtaataa
     7801 gggattgatt tttcatttaa taacctctct ctctttacat tttattccac ttatacccaa
     7861 taccccaaac tgtggaaata tattcttttt ctggtgtcac agcagaaaac ttagctcgta
     7921 ttttttttac tcgttcaaca acagttgagg aatcaccttc agaatcacac ccccatactt
     7981 tttcatagat ttgttctctt gagaaaactt gtcctgcatg gagtgctagt aattcaacaa
     8041 tatcatactc tatttttgtt aaatcaatat tcttaccatc aattttaatt gcacgttcct
     8101 tcatatataa gcataacttc ccaaaataca aagtagatct cttgttctct tcattgatat
     8161 actgtgaacg cttttcccta cgcagattag cttcaatcct tgccataagt tcacgcagtc
     8221 caaatggctt tgttacataa tcatcccctc caaaggtcaa ccctttgatt ttgtcggttt
     8281 ctgattgctt tgcacttaaa aatataattg ggcataaaac atcatctcta attgcctgac
     8341 atacctcaaa tccattcatt ttaggcatca taatatccag tataataagg tcaggctcct
     8401 gttttgataa ctctatacct gcctgaccat cataagctat caacacttca tgtccttttg
     8461 ccttcaattc atcctcaagt agcattacta ggtccatttc atcatcaata atcaatattt
     8521 tactcaatat aacaccacct ttctcttatg ggaaggtcag tattaaaaca atattcgtct
     8581 actaataact tttccttccc tcccacattt taaaccaaat aacgcctcct atcaagaata
     8641 taatcaaacc tattgctact actataagct ctatactagc tgtttgcatc actttagagt
     8701 aaaatgcctc agttgaaata gttccagctt ctttaataaa ataaattcct ggaagcatac
     8761 ctagttttac tggccatgtc catggaacaa atgcccatac tttttcacca acgctagtta
     8821 atccaagtat tgctgccatc aaaattccac cgatactaat accaatagat gcacccatac
     8881 cccatgcaaa actcacccaa agatgaatag ctaatagtgg aagtgtccca atcaccacaa
     8941 gtcctgctgc tgacaggaat aaccatattg ttgctccaga tggcactgca atactcattc
     9001 ctatgcaaag tatcaacgta gcaataaatg tgcatatagt taggcaaaac agaagaaaaa
     9061 gaaattttcc cagatataat ctaacacgag aaatacctac acataaaaaa ccattaaaat
     9121 ttccagcaag ctcttcctcc tgcactataa atcctgctaa aatacctact ccaataggaa
     9181 tgatgaatac agtccatact gtaaaaaatc cttcaaaagc aaattcttgt gtacttccag
     9241 atttagttgt gagataaaaa accacacata atgaaaaaat aataggcata caaagagtaa
     9301 gccactggac agctgtcctc tttgctcgaa gccattctga tgataatagc tttatcattt
     9361 gtcatccctc ctattgaacc aagctgcagt aagtgctgtt aatactataa acacaaccaa
     9421 agaaacaaca atcccaatag gaatcactga agtattcaaa agtgggtctc cagcttctaa
     9481 tatagtaccg ttaggatgta tgttgatgat tgggcatacc attctaactg cccaactcca
     9541 aggtactgca atccaaaatg gttttgtcgc agcaaggaca ccaaatatca tacctaaagc
     9601 tccaactccc atacttagga acataccttt ccatgtagct agccatagct gaagtggaag
     9661 tatagcaaga gaaacaaccc agcatacaat acttgcccct ataatctgtc caaaaggaac
     9721 tggaccagct ttcaatatta aaccagttat aattgttacg actattaaaa ctagcgttga
     9781 aataaaactg tagactgcca tagctataac tttattaatc caaagtgtca taggagacac
     9841 attgtgtgta cgtaaagcac gataatttcc agcttttttt tcctggaaag ctaccaaagt
     9901 agcaaacaat gcaaatccaa taggcagaaa taaaaatgac caccagttaa aaattaatgc
     9961 taaaagattt tgccacgccc aagaagttgt ttgagataat ggattttgca tgagtgttga
    10021 ttgcattata aatgcatatg ctgcaaaaga cactgggaaa aaaacaataa gtttacccat
    10081 aaatgttctt ttatacttta aaagttctga ttgtaaagca ttcatctgtt ttgtcctcct
    10141 ttaagaataa catctgtaaa aaattcttct agattttcat tagggtcagg cattccttga
    10201 aataatagtc gtcctccatt tataattcct atattatcta caacttgagc aacctctgat
    10261 agaatatggc tagaaagaat tacagttata cctttttttg gaaaagaagc aatcaattca
    10321 cgcaattctt gaatgccaaa ggggtcaaga ccattggttg gttcatctag tattaacaac
    10381 tctgggtcac tcaaaagtgc aatagctatt cctaaccttt gtttcatacc catagaaaat
    10441 tgtgaagctc tctttttacc tgtatttttc aaatttacta tttccaacac ttcataaatt
    10501 ttatctttag gtattcccaa tatttttgta tgtactaaca aattttcctc tgctgtaagg
    10561 tttccataca atgcagggga ttctattaaa gaacctattt ttgcaagaga ttctcgtttc
    10621 caaggctttc tatcaaaaat aatacgacca tttgttggac gaagaagacc aacaagcatt
    10681 ttaagtgttg tggactttcc tgctccattt ggtcctaaaa gtccatatat actatttcgt
    10741 tcaatttgta gagaaacatt gttaactgcc atttgttctc catatctttt tgataagttt
    10801 tttgtttcaa gaattaaatc tttcatatta atttctccct tcttgtaata ccttaaatct
    10861 gttactaaga gaatactaac atgaaaaaat aacaattcta taacacctac taattttaat
    10921 aaaatttaaa ataaaaaata gagttaccaa ttttattttg gcaactctat ttaagtttat
    10981 gttttacagt tatattagat ttaaacttag tttcattatc acactaactt caaatttact
    11041 ctataatctt taaatcaaac actaatttat taatcaaaca ctaatctatt aatttatatt
    11101 tctttagaca tctaatatag tttgctacta aataataatt atttatatca tatttatcta
    11161 atcctttaaa agaagttatt tcatcattaa gctcatcact accttctaca aaaacacaac
    11221 ctgtttcaaa ttccatacct acaataatct tacagccact ataaatttcc tcttgagtat
    11281 cgtacaattt ttttgcattt ccttcatttg gaattgtaaa tacaataggt tttaattctt
    11341 tatcactgga aatcttttgt gaaaaaggct tgtttaactt aatattattg ataactacat
    11401 gtttaaattc ctctgaagtt tcttctacta ttacatattt tcgctttaaa aactctatta
    11461 tttctaaaac agacttagaa tttactttta aaatactttt atatttttca tataaagatt
    11521 tccactgttc aattttttcc tgtggtattt cttggatatg tgtacttatt ttttcttgag
    11581 atattttttt caattaaact ctcctttcaa tacttaaata tccttatgaa tctcacatga
    11641 ctaaagttac aaggtttcta cttaatagaa ttttgcatac ttaaatatgt cttattaatt
    11701 ctctacagga tatctctgta attcctacga ttcttacata agttacttat tttaaattgt
    11761 tattagttag attctaaaaa ttatttattt tatcactaat ataatactct taaaaacttt
    11821 acaaatcaat ttaaatttgc taccttttat ttataacaac ttatagtaca tttctcctat
    11881 gcttttcaat atattacttt taaaaggagg ttttcattat gattttaaat agagctgagc
    11941 ttttccgagg atattcttta aatagattta acgagatatg caatatatta tcagtaaata
    12001 aaattaaata taagtataaa gtgaaaaata atgcttactc taaaaaacct ttttttaatg
    12061 aaataacact tggcaattta ggtcaaaagg aagatttctc ttatgagtac ttaatttatg
    12121 ttcataaaga cgattatgaa caggcagagt ctctaataaa ttctaagttg cgatagatta
    12181 tttataaaac atgacattct gttgtcatgt tttatatgtt attatacttt caaatatgct
    12241 actatatttt caaaattgtg tgctttaaaa ctagcttttt atagtaattt aaagcttaaa
    12301 actaaggaga ctcgttatat gaaaataaat ataaaatcaa agttaagtga atttattaaa
    12361 cagaacaatc tttttgatga cagcagaata atatcctacc ttcctaatat aacaatagaa
    12421 acagacaaaa taaaaactat aatgattaat gaggttgtcc caactaacac caatgacgat
    12481 ttttatagtg tagataaaga tgctgattat ttaaaaacta ctattccttt atttgacagt
    12541 gcaggaataa aagtatctaa cataaatgat atcctagata tgggtatcta tataactaat
    12601 gcagtaaaac ttcctaaatc agaatataca ataactcgtg atacaattaa attacatatg
    12661 cctattttag aagaagaaat taaattattt aaaaatctag aggtagtaat gttaatggga
    12721 gatgttgcaa aaaaatcttt taatatgatt actaaaaagc atataaaaaa gaatgtcatc
    12781 ccttccattt caacatataa acttcgtaat aatgaacttt attatgataa tttaagagta
    12841 tttccatcat atattatgac tggtggaaac atactgattg aaaaatcaaa atttgaaatg
    12901 gcatcagaag atataaaaaa aatgttcgag attataaacg gttgatatta caaaataata
    12961 aaaactaata taaaataaaa agtgcctcaa aaacttctat ttatgaggca cttttatttt
    13021 atattagtgt aactatatac tattaacata atttattatt tatattgttc tttttaatct
    13081 ctattccatt tagtctaggt aacattactc tttacaagat tactttatat ttcttttata
    13141 tctctaacgt ttttatttaa taaaacaatt attaatacaa gtcaaatata tctatttatc
    13201 taaaaatata tcttttacag taatatctgg atggtgatac ttcttcctat tcgttaaaaa
    13261 agattttttt acattaatag cctcttttgg acaccagtga aaacaagcta ggcatctttc
    13321 acactcatta ccccactttg gctttttatc aactatactt atattctcta caggacaaac
    13381 ccttttacat gtcccacatc cattacattt attttctgca aaaaatttag tagttgcaat
    13441 ttgcatattc ttacctacta aacgaagtac aacactatct atatgtgact ttaaattgtt
    13501 ttttccttct atatttccaa tctccaagtt attgatacat tttgcaatct cagctaccct
    13561 attagtctga tttttcaatc tttcttcttc aacatctgga ggtgttatca aagcatttcc
    13621 aggcatattg attaaaaaac cagcagataa ttttttcccc ctttttttaa gtaacttatc
    13681 tatagtaaat aaactatgtc cagctacccc attacaagtg gcaatggcat atatatgagt
    13741 attaatatta aaatttaatt tctctacaaa agactttact atatcaggaa catcagcaaa
    13801 ataaacagga aaaacaatta ccaatatatc ttcatttatg tctatattat tttcttttct
    13861 atgtatagct aagggtataa cttctttact tccatttaac gtttctttta aaatatttga
    13921 aactactagc gaatttcctg ttccagaaaa ataataaatc ttactattca ttcttatccc
    13981 tctttctata tttctacatc atcaatagtt aaaaagtctc ttaaaatagg atttatattt
    14041 actttccaat tctatccaaa atatcagtaa acaaaggcaa ttcttcatca gagatagatt
    14101 taaatgtctc tgttgcatat atacttgcaa tttcatttaa tggttcttct aattctctac
    14161 ctaaatctgt taggaatatt tgaaacttac gtctatcttt aacatctact ttacggacaa
    14221 ttaatccctt atttttcata acatctaaga ttcttgtcgt attagattta tccttacatg
    14281 atttttcaca taaatctatc tgagaaattc catcttgctc ccatagaaac cttaataacg
    14341 cataatggtc agttgttata tcatatccat tttgtttaaa catctttttt aaatcatctt
    14401 ttacttttaa cgaagtactt actaacttag ttgtagcttc atatagtttc tcaaaatctt
    14461 tcattttacc tccatttaaa taatcccttg ctcctcccat ctatcaatta ttcccttata
    14521 ttgctcaata tctttaaaag ccattggctt taaaacagca tttatagcaa taaaattata
    14581 gatttgagaa agaacaggtg gatttttctt ttcctctatg caataaataa attttttaga
    14641 taccttttgt atctttgatt ctactttttc agttatagga ttttgattaa atccagtttt
    14701 tatagataaa ctaccacaaa cataacctcc cgaatctaag accatggatt ttataaaaga
    14761 aattgctttt gaaccacgtg cattttcata tgtagtaact gctatacatg gcttacgata
    14821 caataatctt tcaagcatca tatgtgctct atctgtaaac accttgaaaa gtccagttac
    14881 actacttcca taagttgggc taccaaatat aacaccatca ctcttagtaa ttatatcatg
    14941 aatatactct actttatcat tttgattgat acacttgccc attttataac aatttaaaca
    15001 accaatacaa tatgatggat ttacctcact taaatcaaaa taattaattt ttacatctgg
    15061 tattaatctt tctatatctt tatacaagta agttaaaact tttgatgtag ctccattttt
    15121 tctagggctt ccatttataa ctgtaattat catttctata tctccttttt agttgttata
    15181 tcaacaataa gtatatcaac tatattttta gctgtcaata taaaccattt atttaaagcc
    15241 atacttacac agcttaaata aaagttttat ttaactacat gtgcttattt aatcaactat
    15301 aaatgaatta tttttaataa ttgacaaact aaattaatga taatatactt tatgtattac
    15361 aaatgtaagt agaaggaggt atagtatgaa aaagttacca catatttcag aagcagaata
    15421 tcaagttatg aaaattattt ggaaatatgc tcctatcagc actactgaag taattgaaaa
    15481 gttggtagaa acaagtacat ggagtcctaa aacaattcaa acaatgttat tgagattagt
    15541 taaaaaagga gctttaacat atgaaaaaaa tagcagagta ttcgtttata ctccacttgt
    15601 taaagaagaa gaatacgtag ctagagaaag tagttctttt ttaaatcgtt tttataatgg
    15661 tactcttaat tctatggtac taaacttttt agaaaatgat aaactatctg aagatgatat
    15721 tgaggaactg agagaaatct taaataaaag gacgactaag ggggataaat aaatatgtat
    15781 gccccattct ttatacgatt tttactcagt acattaacct tatcaatatt aattgttgta
    15841 attttacttg ccaaaaaagt atttaaaaaa catatttctg caaagtacca atacaatatt
    15901 ggatttatat tgcttataat gttaataatt ccatttgtcc ctcttaaata tattaatctt
    15961 ggaaatgtat ttaactattt atatactttt aatcaacttg gaagcagtac taataggact
    16021 tctatgataa aaaatagtcc aaattctgta attaataact caaatgcatt aaacgatttt
    16081 tcagtatccc taaaccaagt taattttgat agtttaaata ttttattact aatatcatgg
    16141 ataattggaa ttttaatcat gatagctatt actattcatt gcaacattaa aataaaaaat
    16201 atgaaaaaat caatacaaat tttaagagat gatgagatag tatttatatt tgaaaagtgt
    16261 aaaaaagata ttggtataaa taaggatttt atactatgca aatcacctat tataaataca
    16321 cctattacat ttggatactt taaaccacac attatattac cagataagtc tatatctaaa
    16381 ttatctttaa aagatataaa atatatctta cttcatgaat tacaacactt taaaaataaa
    16441 gatatactga taaattatat aatgtgcttc cttcaaatat tatattggtt taatcctctt
    16501 gtatggtatg catttaaaga aatgcgtata gaccgtgaaa ttgcatgtga tatttcagtt
    16561 ttaaaaaagc ttgataaaga tagtcatata gaatatggac aaactctaat aaactttgtg
    16621 gataatattt caagaccatc aaatcttaca cttacatcag atattggtgg gtcaaaaaaa
    16681 caaataaaaa agcgactatt gaagataatg gattttcata atgaatcaaa tacgttaaaa
    16741 ttcaaaagca ttttaatttt tacattgata gctttaattg tatttggaaa tattcccaaa
    16801 ctatctatta tggcagatat caacgaaaaa tatagaccta caaatgaaca aactgtgtac
    16861 gaggacttaa gtcagtattt taaaggtttt gatggaagtt ttgttatgta tgatttaaaa
    16921 tccaagcaat atcatattta caataaagaa aaaagtaaaa caagaatttc tccaaattct
    16981 acttataaga tttatagtgc cttattggga ttggaaaatg ggataataac aagaaataat
    17041 tcacatatcc cttgggatgg aaagcatcat tctaatagtt cttggaatga agaccaagac
    17101 ttattttctg ctatggaaaa ctccgtaaac tggtactttc agactctaga taaaaaaaca
    17161 gggctaaaaa acatacaaac ttatcttagg caaattggat atggcaatta tgatgtttca
    17221 ggtggtatct caaacttttg gctagaatct tcattaaaaa tatcacctat tgaacaggta
    17281 gaattacttg aaagttttta tacaaataag tttaaatttg aagataaaaa tatagaaaca
    17341 gtaaaagatt cattgttaat atcaaaaaag gatggggtat cactttttgg taaaacaggc
    17401 actgccaata taaatggaaa agatgtaaat ggttggttta taggttatgt ggaaaaaaat
    17461 gaaaatactt atttctttgc tacaaatatt caaagcaaag attattctaa tggtagttca
    17521 gcatctaaga taacattatc catattaaat gacaaaaaaa tctataatcc ttaatatatt
    17581 tactgtacat tctaaaaaat tcacataatt ttcatattaa ttatcaatta aattcatata
    17641 caaggcttat tatataaaca tagaagatag taaaactagc ttaaaaacat aatataatac
    17701 cctatacaaa tagtggcaac tctccctcac atcctcaatt gttgccacta tttaattttt
    17761 tgtatgaagt taaatatatt ttcatcattt caacaatttg ggtgcaatac aatctctatt
    17821 ttgaaacaac ctctttaatt gttttagagt ttatgaatta tttctcatac tttaaaaaga
    17881 ttgaattact cttgataatc ttcttttgta gtcattacat atcacctact actcaacaca
    17941 tattctttga cctattttta catccttgtt tatatccaaa atatttacct tcttaccatc
    18001 tggaaacaat accattgtca aagtagactt tcctaattcg tgtattctct ttaaatcaac
    18061 attaacaaga acatctccct gtttaaccat atctccttgt ttaacagcaa catcaaagcc
    18121 atctccattt aatgcaactg tatcaattcc aacatgaatt aatacttctc cattagttcc
    18181 tttaatccca aatgcatgtc cacttgaaac cactgtttct aatttacctg aaataggaga
    18241 aacaattttt ccatctataa tatcaatagc aaaaccatca cctaacattt ttttagaaaa
    18301 tgtttcatct ggtacatttt ctaatgcaac aattttcccc ttagctggag acacaaagtt
    18361 ttcattattt tcattaaaca ctaattcttc gtcttcatct tcatttatag cttctttagg
    18421 cactccaaat aaacatgtta caataaagca gaatgcaacc acaaatattg atactaataa
    18481 gtatgtgatt aaattatatc catcatagat atacattaaa taagaaggta aaactgctaa
    18541 tccatatgaa ttagattgaa tatgtaaaat tgataatatt gctgctccta aacctgaaga
    18601 tgcacacata ataatcaaag gctttaaatt atatcgaagc tgaatcccaa ataaaacagg
    18661 ctcacttata ccaaaaagtt gagatagcat tgctccaacc gcagtttgtt taacttgatt
    18721 atttttagat tttttcataa atgctagaca tgttccaata ttcgcaaatc catacattgc
    18781 acaaagagta ataagggcat taaatcctgt atttgctaat agtgatgttt caatcattac
    18841 ataagtatga tgtaagccag taataacagc taatggatat gtaaatccaa taataaatcc
    18901 tccaattcct aaaggcaaat caatcaataa gctgatgata tcaaccattt ttaattctat
    18961 tgtatgcatt atagggccta cacctaacaa aataactaac attgttatta gcataacaaa
    19021 aaatggtgtg aaaataaggt ctaatacatt aggcatatgt ttacggaacc atttttcaag
    19081 attagctcca ataaatgctg taacaatagc tgttaaaaca cttccttgac aacctacaat
    19141 aggaataaat ccaaatgcca taattgcttc aatttcattt gatgggtcag caacagaata
    19201 tgcattagga agtattgggg aaactaacat aagtccaata acaagtccaa taacaggtgt
    19261 tcctccaaat actttaaatg ctgaccatac aataattgct ggtaaaaaag caaatgctgt
    19321 ttctgtaaga actgaaacaa gtgtctgaat atacaatgga atatttgcag aagacattcc
    19381 taataatcca agtacattat cattaaacaa acaacccttt aatcccaaga ataatcctgt
    19441 tgctgcaata actggaataa taggaacaaa tatatctgct agagttctca taactttctt
    19501 aaatccagtt tcattatttt taactaattc atcttgttct ttttttgata aagtttttaa
    19561 tcccattttt tcaacttctg cataaacttt attaactatt cctgttccta aaataatttg
    19621 atactgtcca gaagtaaaga aaaccccttt aacctcatca actttttcaa ctttcttatc
    19681 attaataatt tctctatctt taaccatcac tcttaatctt gtggcacaat gtgtaataga
    19741 aataatatta tcttcaccaa taattttaat aagctcattt gctattttat tatatttatt
    19801 catttttcta cctcgctttc aataatactg catgaaatgg tttcaaacac actgttttat
    19861 ttaatagatt tatatcatct aaattttgga atacaatatc atatttttct tctactctta
    19921 tttctacagg tttatcagaa aaattataat aacaatcaat aactaccaaa tctaattgcc
    19981 ttctatacgc aataacatct tcactttcta tatcaatagg ttctattgaa ccataaatca
    20041 aacaatcaga atactcacta tcttgtctca actttatcat ctttttataa aattctaaaa
    20101 tactctctga attgttaatc tgactctgtg cattaatatc tttataattt tcaataacct
    20161 ttaaccatgg tgttgtatct gaaaaacctg cattttcttt atctgcccat ggaaatggcg
    20221 ttcttgcatt atctcggctt cttaaattca caaattctaa cgcttcttct tctgagtaac
    20281 cttcatctat tgaacgatag tattggtcaa tgctagataa atcattaaaa tcatttatgg
    20341 tatctctttt aaaatttgtc attcctaatt cctgcccctg ataaataaat ggggttcctc
    20401 ttaaaaagaa atacattgct cccaacattt ttactgcatc atcattcctt tcgtattgca
    20461 aaagatactt tgttgtagct cttggttggt catgattttc aataaaatta gctgcccatc
    20521 catgttcctg aacatacatt tgactatcta aaatgcattt tcttaaatct ggaattgtcc
    20581 atgaaattct tttaaaccac tcactaccag aagcaacatc taagtcagca tgtttaaagt
    20641 caaagaccat tgaaaagaaa ccatcttctc caataaaatc acctagctgg tcatagctaa
    20701 tacctgctgc ttcagcaaca gtcatacaat catatttttt aaacgtattt tcttttaatt
    20761 cataaagcat tttttctatt ccaggctgat ttctaactgt ttttgtacat ttaacaagac
    20821 catcaacacc atcactttct aaactttgaa aacttaaatt ctttttgata aatgtaatag
    20881 catcaactct aaatcctgcc actcccattt gagaccaggt attaatcatt tggtaaagtt
    20941 cacttcttaa tattggattt tcccaattaa ggtcaggttg ttttttacca aaagaatgaa
    21001 aataatattc gtctctattc tctacctttt cccaaacact tccaccaaaa acagacctcc
    21061 agttatttgg taaatcgttt ccttttttaa aaatataata atcatgataa ggactttctg
    21121 gattttttaa tgcttcttga aaccaaggat gttcatcact tgtatgatta agaactaaat
    21181 ctaatataat tttgattcct ctatctttac tttctttaat taactctttt aaatcttcca
    21241 tacttccaaa ttcacttgca atatccaaat aatcactaat atcataccca ttatcgtcca
    21301 taggtgattt aaaaattgga cataaccaca atgatgtaat acctaaatct tttaaataat
    21361 ctaaacgaga aataattccc tgtaaatctc caattccatc attattacta tcttgaaagc
    21421 ttcttggata aatttgataa acgatttctt tttgccacca tttcttttcc ataaattact
    21481 cctttttata tgatatttct tctttttaat tctttataaa ttccattatc gaatatgtcc
    21541 tcacatatag atgtcgctat atctttaagt tgttgatgac tatttttcat tgcaattgta
    21601 acgtctgatg cttgaaacat gacaatgtca ttttgaccat caccaaaaca tatggtttct
    21661 ttttgagtta ctcctaatct ttcttgtaat ctcttaattg ctttgccctt atgaaaatct
    21721 ttttgaataa tttcataata ctgactagaa atatctctct gtgctaattc cattttatct
    21781 tgtagtatat ctttcacttc gtcaaatacc ttttcatttg accataaaca aattttatgt
    21841 atatcttgtg acttatactc ttcaatgtta ttttcatatg ttattttttc ttggatatgt
    21901 tgtttattta tacaagaatt agttcctttc aactgattca ttgtttcaaa tatttctttg
    21961 gctttttgat tcatgaaaac tttttcctga ctctcaatcg aaaaagcaac ttctctcttt
    22021 tttaataaac aaacaacttc ctttatcaat ctttgattaa aagattgatt ataaagtaat
    22081 tcaccatgat actgaatgta attacctcct cctgcaatat atccatcaac tcctaatgat
    22141 aaaacatcat cttgtattgt acccatactt cttccagtac aaataacaac actgcaatga
    22201 ttcttttgac ataaacgtat agcatgcttt gcagattctg ggattccata tacctcatct
    22261 ctaagcgtac catctatatc aagtattaat aatttgtaca atttcatctt cctttcttac
    22321 tatcaaaatc ataatagcat tcaaaaaaaa agatatcaat attttgatat cttttaaaaa
    22381 atatgttcat ttttagcaca aattttcatt ttcggggaat atgttcactt tctaaataca
    22441 tcttgtattt aatataaagc agttcaatta aaataaaata aggtgttgtt acatcgtagt
    22501 ccagttgttt atctacagaa atagatatag attgaatata caggttttca tcacataatc
    22561 ttgctaaagg attacttatc atttttgtaa tagaaaccat ataaattcct ttcattctca
    22621 tttctttagc aaaatcaata attgtagatg catctccatt caatgaaata aagaaaatca
    22681 tatcatctgt ttttataaaa tgattaagtg cctgaatcat atcataacca taaacatcat
    22741 atataatttt ccctgttggt aagaaaatcc ttttcatctc tttagcaact ctttcctgag
    22801 aataaccttc tccaaagacg attatacgat ttgccttttt cattctttca aacataatcg
    22861 aacaatcctt cttctctata tcatctatca ccttattata acaatccatt aattggtgtt
    22921 tactttgatt tttatattct acttctgttt catttaagcg tataataact cttaattcac
    22981 tatatccagg taattgtagt ttttgagcaa aacgagaaag tgcacttgta gatatatggt
    23041 atttgtctgc aaattcatct atagaaagtt taatacaatc ttctttatga ttaagaatac
    23101 actcacagat atacttatca tttgctgtaa atgaattata atatttattg aaaagtcctt
    23161 caattttcat tcttttctcc ttctatactc tatgtaattc atatgaagca aatctaatag
    23221 agcataaaat gctcctacga cttcataaga tatagcttgt aatgtatcaa gtttttgcgt
    23281 ttcaacatac agattatact cacaaagtgt tgcaattgta ttattatcaa gcctagttaa
    23341 agaaatcgta tgtatatgat tgttgataaa ttttgcaatt tcaattcctg cttttgtttc
    23401 tccagacaaa gaaatgataa taaataaatc acttttttga aatgattctc tcataaattc
    23461 aatttcacct atatcaaata agtcaataac acatttacct actgaaagaa atattctctt
    23521 tatttcttga gctaaacttt tctgttcatt acctgtccca taaatataaa ttgtttctgc
    23581 atcatatatt attttacaaa tatcttgaaa tgatattttt tgtaattcat tcattaatag
    23641 tcgataattc tcgcaaacat catgaaaatt attatcatta ttaacaaatt ctagttgatt
    23701 atttttaagt aaatacttta aatcagagaa tgaatctaat ccaatttttc tacaaagcct
    23761 taataaagta gctctagaaa cattacattc atttgcaatt ttttcacacg aaaaattacg
    23821 gtattctctt tgacaaatca atatttgttt aataataatt ttatcatttt catttaaatt
    23881 atcataattt tggttaacta acgattctaa tctcattttt tccactccct aaatattatt
    23941 ttaatataca ttagttgctt tttctaggga agagttttaa ataataagga taaaataatc
    24001 atttgatttt atttttatta aaataaaaat aacctgctaa aagcaggcac caaaaagtac
    24061 ttatcctttc gaacatgtac tttagatatt gctttcttca gaagcttcat ctcttcatac
    24121 tctaaaatag agttttttct aactcaaaag tgttttccat ggtagaaatt actttaattc
    24181 atctgccatt tactttttat cttatttaat aattactatt acttgaatat ttacttcttt
    24241 aagtaaattt catagttaac aaagctaaaa ctattattat agatgttaaa atacaaaatt
    24301 gtaagtattg ttataaatat gcttacagct acaaatatac atgatatatt aaaaagaagc
    24361 tgtaaattat tatcatacat acaagcaact atgcctctat taatccatgt taatttttat
    24421 tatttaatac aaggagttcc atgagacata catatccatt catattttaa attatccaat
    24481 tcgcttatag atttcatcat ctttttataa tcccaattcc atggattagg ataaggaact
    24541 atatttccat tcttattttt gactaaatca cctgcaaata atacatcttc aaaaatcatg
    24601 cagacatgcc ctggagtatg accaggagta tggataattt ttatattccc aatctgcata
    24661 ttctctccat aaggttcaac atccttaatg agccttctgg aaattgcctt tccaataaac
    24721 ttcttaaacc caggtctatc cttctctcct ataataaaag gtatatcctc tacatgagcc
    24781 catacttttg ctcctgtttt ttctttcaat aatttaatat tagatatatg gtcaacatca
    24841 tgatgagtta ataaaatatg cttaatatct gtaagtttaa tgccaagtgt ttttaattct
    24901 ttaattatat tttcccccat aaaaggaaga cctgtgtcta taagaataat ttctttatca
    24961 aacacaataa aacaatttga gccacgagta ctatctaaca ttaatacatt ttcttttatc
    25021 ttcataaata aattcccctt atttggtatt ttacctatat aattaattaa taaagttata
    25081 aatagttata atacttgata ttaattttat tttttattaa agtatactat aggtatacaa
    25141 gtattatatt tacataagta taccatcagt atactttttt atcaatagaa aggaataatt
    25201 tatatgtcag aattatcaaa aagagataaa gaaaaaatac aaagagaaaa tgagattatt
    25261 gataaggcag aaaaattatt ttgtcttaat ggatttgata atacaactat gaatgaatta
    25321 gcaaaagaag ttgaatatac taaacgcacc atatacaagt acttttcatg taaagaagat
    25381 ttattctttg ctgttgtctt aagaggatat aaaagacttt gggataatgt taaaatagaa
    25441 agtgcaaaag gcaaaacagg gtttgaaaaa ataaaacttt catactttgc ttttcataaa
    25501 ttttactctg tagaaccttc tctattatgc cttatgggaa tgattggtat tgtaaactct
    25561 gaaaaatcgg atactgatat gctgtttaaa gaaaagtttt tttcttttaa taaatttatg
    25621 tttgatgaaa tacaaggaat gtttgaaatt ggaaaaaatg atagaagtat taggcatgat
    25681 attgaaatac ctactcttat gtattcatca atatttacac ttacaggatt ttttaattta
    25741 ctatctgtaa caggaaaatc atacctgaac aactttaata tagatgagga aaaatttatt
    25801 gaaacaactc ttgctctttt aatagattca attaaagcat aaacctaagt ttataaatta
    25861 aagtttatat acttaaatga taattttttc tatctctaat ataaaaaata ttaaacataa
    25921 aactaataac ttagttaatg gaataactaa attactgaaa ggattaaaag taaatggcta
    25981 aaatacttct tttagaagat gatgatacaa ttacatttgg aataaaaact tcccttgaaa
    26041 agaaaaatca cactgtatgt tgttataata cgattgctga agctaaaaaa aattttgata
    26101 aaactattga tttaattctg cttgatttaa atctacctga tggtattgga tacgagtttt
    26161 gccaatatgt taaaagtata gaagatactc cagtgatatt tttgacagtc cgtgatgatg
    26221 aaaaagacat tatacgtgga ttagatatgg gtgcagatga ctatgtggtt aaaccgtttt
    26281 tgctctcagt acttcaatcc agaattaatg cagttcttag aagaactcat tcaaatacta
    26341 acaatactat tacatgtgaa aatataagct tgaataaggt tgaagtcaaa gcatatttag
    26401 ataatgttga agtacctttg acagcaggag aatataaact tcttctaagt cttatggaaa
    26461 ataaaaatag agctatgact cgtacaaagt tactagaaat gctctgggat gtagatggta
    26521 atttcgtaaa tgataacact cttactgtta caataaaacg tcttagagaa aaattaaaaa
    26581 atccaccttg tataaaaaca ttaagaggca taggatatag aatggaggac aactagtgaa
    26641 aactccaaga aatatcaaca gtacagttgt ttttataata ttctgcttaa ttgtaacttt
    26701 tttattgagt gtaattctaa caactataac ttcttttaat gaatacaatg tactgtctaa
    26761 ggctcttggt gcatcatatg agtacaatcc taagtctaca gaaagtatac taaaatcact
    26821 gaaaaattct agtgataagg attttaaaac aggaaatgaa attttatcaa aacatggata
    26881 ttctaaaagt acattttgga gtaaaaactt tttttatttt ctagtgatat cgtttggtat
    26941 aatttcaaca atggctacag ttgtatatat aatcaaacat agaacaaata agcaaaaact
    27001 gagtagaata aacaatctta ctcaatactt agaagaagtg aatttaggaa aagagtctgt
    27061 acttttacga tgtgaagatg aattttctca ccttgaagat gagatatata aaacagttgg
    27121 ggaacttcgt tgttcaaaag aatttgcttt aaaagaaaga caaactcttt cagataatct
    27181 tgcagatatt gctcatcaac ttaagactcc aattacttct atgtcactta tggcacaatt
    27241 actttcagaa aattgtccag atgaagaaat agattatatt aacagactta caaatcaaat
    27301 ctcaagactt gagagactag tttcatcttt acttacatta tcaaaattgg atgcaagtac
    27361 tttaatattt gaaagcaaac ttatagatgt acattctctt cttacttatg caattgagcc
    27421 tatagaaggt tctttaagag aaaaacatca gacatttact atcgaggctt ctccttcggt
    27481 agaatttgaa atcgatatta attggacttt agaagctctt ttaaatattc ttaaaaattg
    27541 tagtgagcat atagaagatg gtggatatat agttgcttat tatagtcaaa atcctctata
    27601 tgttgagatt atcatagaag ataatggtaa ggggtttgct caggaagaac ttccatatat
    27661 ttttaataga ttttatcgtg gtaaaaatgc tagtaaggac agtattggta tagggcttgc
    27721 tctatcaaag tctattatag aacgtcaaaa tggtactata catgcagaaa attgccataa
    27781 tggtggtgca agattcataa ttaaatttta cacataaaat gctggattat aggtaaaata
    27841 cctataatcc agctatatct atattctatg ttatctattg ttttagtaat atacttctat
    27901 ttacatttaa ggttttatta aacaaacaac ttaactatcc ctatacttgt aagtagtctt
    27961 aaatcgaaac ctttaaataa gttcaaatag attttgttta tattgtttca ttttttatag
    28021 attctactat attttcattc ttcaactgct tgtatcctgt catatatgat gctattacaa
    28081 taaaaagtat caatatagta aaacctatta ttgtagatat aggtgcaaat ggtaaatact
    28141 ctataaaata tatttcattt atacgtagaa aagcatacac tattcctatt tgcacaggaa
    28201 tactaagtag taatggtgta attcctaaga ataaaccttc caaaataagc atctttttta
    28261 tgccttctgg agataatcca acagaacgca acatagcaaa ctcttgtcgt ctacttctaa
    28321 tatttcctga cactgtagca agtacatttg ataatccaat catagccaat aaacctgtaa
    28381 taaagcctac tattaaattc atggtcttta ttccactagc atcttgtttt tcactttcta
    28441 aaacatcctc aatactataa tctcctgaac catagtcttt attataaatg gactctaatt
    28501 tttctcttac tctaggaatg tcctcacgat tatttacttt aaatactcct atcagcctat
    28561 ttgaacgtaa cctacttact tcatcataat ttttagatat ttcctgcacc atagacattg
    28621 gaactatttg cattaatgta tactttgaac tagtatctac tattttagga agtgtgcttg
    28681 taatagctcc cactttcatg ttgtaagtat aatccccttt atcttcgtca tatacttttt
    28741 catttaattt aatgttatct cctattgaaa gatttaaata atctatatat actttatttc
    28801 tacgtgtact acgattaata tcttcctcct tattatatac aattgaggtt ggattttttg
    28861 tatcataaaa taactttggg tctacagaaa tttgcctaca atattctgta aaactcttat
    28921 catcaagccc aattatattt gaaattatac gaaactttcc atcacgttct ataggagagt
    28981 actttttaga tgaagctata tttttaaatc ctccattttt cgcaagttct tttgaagcat
    29041 ctttttcagt caaccaagtg gaagcagaca ttttttgata atatagtgaa ctttttacac
    29101 cttctgtatt atctaatttt tcaatgaaag cagactctac aggttcacta ttttccaaat
    29161 aaactgctat atctctttgg tccttataat tatcacttcc ataaacagct tttgctcctt
    29221 cttgattggc aattatatgc aaaaaacatg taagtaataa aaaagataat gttaaagata
    29281 ttgttgcagt acgataagat tttttacgag cacgtagtgc gtttgatgca agttctcctt
    29341 ctattccaaa tatctttcct aatgaatatt tatgcgactt tttgatttta acactatctc
    29401 cttgtcgtat tgcttctata ggagatatct ttgatacttt tcgtgctggt ataagtgcag
    29461 aaatccagac tgttactatt gtaagtaaaa ctgctggcag aaaagcagga ataccataag
    29521 taaataccac ttctggaaca tcagtatgat aattaactga attaacataa actataagcc
    29581 tattacacaa taaccatcct aaaaataaac ctattggtaa tggtataatt gtcagtaaaa
    29641 agccttcaaa cactacagac cttttaattt gttttggtga tgcaccaaca ctagataata
    29701 tccctaactg agtaagtcta gcatttgcag acagtgaaaa cgcgttgtaa atcaccatta
    29761 caaacacggc tactgtaaaa attgctattg taagatacat aataggagtt gaaaattttg
    29821 atatactact catagatgct ttctgttctg gtgaaaagac taacatcttt aaaagataat
    29881 tactattgta tttaagattg tattttccat attcattagt cttccatccc aatgatttag
    29941 caagttttgg aagttcctta tacgtgtctc ttatattttt aaatcgcaag tacacagtta
    30001 tactatcatt aggttttata cttgttttat caagatatcc aagagctgta tatgcaggta
    30061 ttactgatga agtagaaaca tctacctttc ctactattgt caatgtagcc tctgaaattt
    30121 ttttaaaact ttcccctttc gattttgggg ctatgggttc aagaggcttt ccatttagga
    30181 ttctatctcc tattgggaga gtaattttat ccccaatttt tacacttgga ttattctcaa
    30241 aatattgttt tgataatgca atttcattag cattatttgg tcgacgaccc tctattatag
    30301 tatccttttc tggcatagaa ttccaatact ctgatgtagc atttctataa attatataat
    30361 ctcttttctc atcattaatt ttgcctacat accattgtcc ttttaccatt acatcatcaa
    30421 ctgaagaaaa attttcaatt aatggtaagt cacttccata agtatagtca aacaattcac
    30481 catgccagtt accttctttc tcaattgaaa gttttataaa atctgtccac atggtataca
    30541 acagtccaca caaagaagac atcatggttg tagtaagtaa tatagctaac attatagcta
    30601 tactactacg tttattgcgt cgtacaaagt ctaaggtgta ttcttgaaga atattcattt
    30661 cttcaccacc ttttcagaga caatcactcc atcttccata gttacaatac gatttgcctc
    30721 tagtgctatt ttctcatcat gagtaataag aagtattgtc tgattaaatc gtttatttga
    30781 taagtttaac aaatctacaa tttcttctga attttttcta tcgagattac cagttggctc
    30841 atctgctaat aaaatagctg gtctataaat taaacttcta gctatagata ccctttgttg
    30901 ttgtccacct gaaagttgac ttggaagatg gtttaatcta tcagataatc ccaatataga
    30961 tacaatctca gagaatctat cttcatccac ctttctttta tcaagtaaca tgggtaataa
    31021 tatatttttt ctaacatcaa gagtaggtat aagattataa aattgataaa taagacctac
    31081 ttttctacgt ctaaaaactg caagagcttc ttcctttaaa cttgctattt ctgtatcttc
    31141 aacataaatc tttccagatg taggtctatc tactccccca agcagatgta acaatgtaga
    31201 tttgccagaa ccagatgctc ctacaatgga tacaaaatca cctttttgaa ctgataaatt
    31261 tacattatta agggctgtca ctctggtttg atttgaacca tatatttttg tgagattttc
    31321 acattttaat atttccatat acaatcatct cctttatggt taaattttag caacctaaaa
    31381 tgacattata gtgacatgaa tacaaatata aattactatt aaaaatattg tttagttttt
    31441 gcacattatt taaaattata atataaaaca aagacta
//
LOCUS       DAEOSP010000025        30961 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.025, whole genome shotgun sequence.
ACCESSION   DAEOSP010000025 DAEOSP010000000
VERSION     DAEOSP010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30961
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.025"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..171)
                     /locus_tag="KRH54_000923"
     CDS             complement(<1..171)
                     /locus_tag="KRH54_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578592.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIK"
     gene            complement(186..629)
                     /locus_tag="KRH54_000924"
     CDS             complement(186..629)
                     /locus_tag="KRH54_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578593.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSKNTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            complement(630..1502)
                     /locus_tag="KRH54_000925"
     CDS             complement(630..1502)
                     /locus_tag="KRH54_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBG8578594.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKGHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYISNIGMII"
     gene            complement(1555..1914)
                     /locus_tag="KRH54_000926"
     CDS             complement(1555..1914)
                     /locus_tag="KRH54_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBG8578595.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            complement(1968..3053)
                     /locus_tag="KRH54_000927"
     CDS             complement(1968..3053)
                     /locus_tag="KRH54_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBG8578596.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKAKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANIGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            complement(3224..4603)
                     /locus_tag="KRH54_000928"
     CDS             complement(3224..4603)
                     /locus_tag="KRH54_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBG8578597.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            complement(4596..5276)
                     /locus_tag="KRH54_000929"
     CDS             complement(4596..5276)
                     /locus_tag="KRH54_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBG8578598.1"
                     /translation="MKKQYVFIILTSTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            complement(5668..6747)
                     /locus_tag="KRH54_000930"
     CDS             complement(5668..6747)
                     /locus_tag="KRH54_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8578599.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     regulatory      7035..7150
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7325..8695
                     /locus_tag="KRH54_000931"
     CDS             7325..8695
                     /locus_tag="KRH54_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8578600.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     gene            complement(8960..9571)
                     /locus_tag="KRH54_000932"
     CDS             complement(8960..9571)
                     /locus_tag="KRH54_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBG8578601.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(9561..11924)
                     /gene="lon"
                     /locus_tag="KRH54_000933"
     CDS             complement(9561..11924)
                     /gene="lon"
                     /locus_tag="KRH54_000933"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBG8578602.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            12104..12616
                     /locus_tag="KRH54_000934"
     CDS             12104..12616
                     /locus_tag="KRH54_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG8578603.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            12604..13131
                     /locus_tag="KRH54_000935"
     CDS             12604..13131
                     /locus_tag="KRH54_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBG8578604.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(13202..14452)
                     /gene="clpX"
                     /locus_tag="KRH54_000936"
     CDS             complement(13202..14452)
                     /gene="clpX"
                     /locus_tag="KRH54_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBG8578605.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(14471..15055)
                     /gene="clpP"
                     /locus_tag="KRH54_000937"
     CDS             complement(14471..15055)
                     /gene="clpP"
                     /locus_tag="KRH54_000937"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG8578606.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            complement(15205..16491)
                     /locus_tag="KRH54_000938"
     CDS             complement(15205..16491)
                     /locus_tag="KRH54_000938"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBG8578607.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            complement(16685..17155)
                     /locus_tag="KRH54_000939"
     CDS             complement(16685..17155)
                     /locus_tag="KRH54_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG8578608.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            complement(17155..18504)
                     /gene="rph"
                     /locus_tag="KRH54_000940"
     CDS             complement(17155..18504)
                     /gene="rph"
                     /locus_tag="KRH54_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBG8578609.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNIPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            complement(18664..20688)
                     /gene="ligA"
                     /locus_tag="KRH54_000941"
     CDS             complement(18664..20688)
                     /gene="ligA"
                     /locus_tag="KRH54_000941"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBG8578610.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            complement(20874..21812)
                     /locus_tag="KRH54_000942"
     CDS             complement(20874..21812)
                     /locus_tag="KRH54_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBG8578611.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            complement(21914..22336)
                     /locus_tag="KRH54_000943"
     CDS             complement(21914..22336)
                     /locus_tag="KRH54_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBG8578612.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            22524..23807
                     /locus_tag="KRH54_000944"
     CDS             22524..23807
                     /locus_tag="KRH54_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBG8578613.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKNLNKERLDKLMQ"
     gene            complement(23885..24400)
                     /locus_tag="KRH54_000945"
     CDS             complement(23885..24400)
                     /locus_tag="KRH54_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG8578614.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(24400..25776)
                     /locus_tag="KRH54_000946"
     CDS             complement(24400..25776)
                     /locus_tag="KRH54_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG8578615.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            complement(25801..26352)
                     /locus_tag="KRH54_000947"
     CDS             complement(25801..26352)
                     /locus_tag="KRH54_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG8578616.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            complement(26410..26625)
                     /locus_tag="KRH54_000948"
     CDS             complement(26410..26625)
                     /locus_tag="KRH54_000948"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578617.1"
                     /translation="MQIHRELSKSENEYMCTKKEKQRYSKCEICGVLIDDTDKVYNNT
                     YNLCKKCKRNISTEKIVKYINIYDESK"
     gene            complement(26594..27427)
                     /gene="fdhD"
                     /locus_tag="KRH54_000949"
     CDS             complement(26594..27427)
                     /gene="fdhD"
                     /locus_tag="KRH54_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBG8578618.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDKNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            complement(27476..29620)
                     /gene="fdhF"
                     /locus_tag="KRH54_000950"
     CDS             complement(27476..29620)
                     /gene="fdhF"
                     /locus_tag="KRH54_000950"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(29204..29206),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBG8578619.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            complement(29871..30929)
                     /locus_tag="KRH54_000951"
     CDS             complement(29871..30929)
                     /locus_tag="KRH54_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG8578620.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
BASE COUNT         9317 a         5542 c         3342 g        12760 t
ORIGIN      
        1 ctttatttga taattatctt gattatttat tttatttatt atattttgct tttcattttt
       61 caaactagtt aggttttctt ccaaataact tactgctata tcacttgcca atataataac
      121 taatattaac aatattttaa gtagattttt ttctctatta tttaatctca aaaataatca
      181 cccatctaac tttttattct aaatctataa tattctccat ctcttacaat actgtctatg
      241 taataatatt ttatgtactt tattttcctt aggttgttta aaacatctaa atcactacac
      301 acaccttcca tctctagctt atttttatct atcgatattt tagtaatact atttattcca
      361 attgtttcat atattttttt tatttcatta agttttattt tagaaaattt ctctctagtt
      421 ttatctgttg ttggtaaagt attctttgat ttatccacta gcttattttg acttatataa
      481 tcttcaagtt catttataga tttcacatac ataaatgatt taaataaaat acacgttaaa
      541 attaagacta ttattatttt atagatttta ttacatggtt tagttttttt aaatccatta
      601 agaaaattta ttgttttatc cactttcact taaataatca ttcctatatt acttatataa
      661 tttatcctat tgtcttctaa atctgcatca aaattatttt ctacttttac tttatctttt
      721 aatcttaatg gatttacttc aatagattta tctgtatatt taaagtattc ttcaataaaa
      781 caattttcta taccatagta ataaacatac ttaaaatccc ttaataatct ttttatgaac
      841 tcatatgatt ttccctcttt tgatagcaag tatgtttctc ttattttttt atcttttgtt
      901 atatttataa ttaactgact ttttttacat tctataaata ccccttcttc tgaaaaattc
      961 tctattaaat ttaagtttat aagcttttgt aatatatcaa aatttatatt tatagtcttt
     1021 ggattcatat ttagattttc tgaaatgttc ttaattaatt gaataatact ctttgggaaa
     1081 agaattactt gtatattcaa tttatcattt ttagaattta taactttata ttttattgaa
     1141 tagttattta tgtttattgg aatgtattgt gttatttcaa attcaatcat gcttaatatg
     1201 tctctttttt tcttagtatt tagaacttca acatttctaa tgactatatc ctgaatttga
     1261 atgttaaagt ataaattttt atataatatt tttgatttac caatctcctc ttttacaaat
     1321 ctaactacat cttctatata cttaatcttc tcaatcgaat tactatcatt ttttatatca
     1381 aagttaatct cttttataat tgatttatct ataaaaactt ttttatgacc ttttcttaaa
     1441 aatacaagcc ttatataatc atcatttatt tctaagttta gaattttttt aattatagcc
     1501 atctccttct taaataaaaa taattttaaa acttacagca atcaatataa aatattattt
     1561 tggataaaat tgcttagaat ttatatttac gcttatatct gttccgctat catttatgac
     1621 aagttcgaat ttacctgatg tactctgagg aactggaaca gtatttaggt atcccttttc
     1681 cttcaatgtc tctacactta gagagtctat tgtcttttca tcatcattaa ttgccatgct
     1741 agctgcggtt actatattag cagcattcgt atagtctgca tttaatttgg cactttcttg
     1801 aaccttacta tacttaacta atgctatact agatataacg cctaaaatag ttactactac
     1861 aatcatttca acaagtgtaa atccttttcg ttttttattt atcaaattta tcaattaaat
     1921 attctctcct tttcaaaagt ttcatctaat aaaatttatt tttctctcta aaaattagta
     1981 attgcatcaa acataggaac aaccattgct aaaataaaag taccaataat taatcccata
     2041 aatacaatta gcataggttc tataaatttc attatttgct ctattttgtt atttaattca
     2101 ttttcataaa atttatttat tacatttaga cttttatcta gtttcccact ttcttctcct
     2161 atatttgcca ttgaaataaa taattttgga aaaacatttg ctaaattcaa agatttacct
     2221 atgctatttc ctttttttat ataattattg gaaatcttta acctttcata aataaattcg
     2281 ttgtctataa cactagctga catgtctata gcttccacaa tttgtattcc actgctaatt
     2341 aaaacggaca atgcccttga aaatcttgtt gtagctacta actgattaat tttttttata
     2401 aaaggtattt tgaattttaa cgaattaatc aattttttta tattatcatt tgttataaag
     2461 aaataaatgc ttcctattga aaacaatata aaactaaata ttatataagc taagttattt
     2521 gtaaccactg tagatattcc cattaaaact ctagttatta aaggtggttc aattccattg
     2581 ttggtaaaaa ccacctgaaa attaggtatt acaaatacga atataaacaa cattgataag
     2641 atagataata ttataagtat tattggatat attgaaatag ttattatttt catttttaat
     2701 ttgtattctt tatcataata attagataaa tcactcatta ttatatctaa gtttccacta
     2761 gtttctcctg cttttatcat acttataaag aatttagaaa atatacctgt tatttgaaat
     2821 gattctgcta ttgagttacc tttttgaata tgatttgata ctatactaag caaattcttt
     2881 gcttttttac ttgactgact ctcaatgatc tcaaatattt ttgtaatttc acatccagat
     2941 tccagcaata tgcctatctc tttgcaaatt actctaattt ctttatcact gattctattt
     3001 aatactaaat aaaacttttc taaattataa aatatatttt tagataatct catgtactat
     3061 aataccttcc aaattatatt aattacaatt aaataaactt aatattttct aatttaatgc
     3121 atttaatttt atttcactta aatataattt atactcaaac atcttaatta aaatagatta
     3181 aataaagaca actgaccaaa cgaagtttaa ctatacaatt cagttaaatc atttcattta
     3241 ctactaaaca ctcttctaat gtcgttattt tttcatttat tagtctttta caactatctt
     3301 caaatgttat cattccatta ttttgtgcca catctttaat agtactagaa tcctccatat
     3361 ttcttataca tgtctttata tcatcattaa tttctaagat ttcataaatt gctgttcttc
     3421 caaagtatcc tttatcatta catttttcac accctacagc taccttagta tttacatctc
     3481 cactaaaatt atcttttatc ataatctcat ggctacaatg attgcagact tttctaacca
     3541 acttttgaga tataacacct ataagagagg catttaaaag atatggtggt atttgcattt
     3601 ctagtaatct agctatggaa gatactgcat cattagtatg taaagtactt ataactaaat
     3661 gacctgttat agctgctctt actgctatct tagctgtttc tgcatctctt atttctccga
     3721 ccattattat atctgggtct tgtcttaata tcgctctaag acctacatca aagctcaacc
     3781 ctacttttga atttacttga atctgactta tgccatccat cttatactct acaggatttt
     3841 ctatagtcat aatattttta tttattcctt ttaaatcatt taataaggaa tatactgttg
     3901 tagtctttcc actacctgta ggacctgtca ctaataatat tcctgctctt ttatttatta
     3961 tacgatttat agattttata gcttcctctg aaaaaccaag ttctaatttg tcttttaaaa
     4021 aagtatctct attaagtatc ctcaaaacta ttttttcacc atagacagtt ggtattgatg
     4081 aaactctaat gtcaattaaa tttccaccta actttatatc caccctacca tcctgaggaa
     4141 gccttttttc tgttatatcc attgatgcct ttaattttat aaccgtagac aatgatggat
     4201 agacatcaac taaaaacttt gatacttcct ttagttctcc atctaccctc attctcacta
     4261 ttaaatgttc tttaaatggt tctatatgta tatcacttgc gttttctttt atagcttttt
     4321 ctaaaagatt ttcaaataat ttttttgcat aatcctcatc aagttcaaag ctattataat
     4381 atttttcaat cttttcactt ataagttctt ttttctcctt cttcaatata atttctttac
     4441 ctgtagctaa tctcaagtcc tgtagagcat aaatatcctc atcttctatt tctacaacta
     4501 aattatcatc ttgaaactca actggaaata cattatattt ttttgcaagt ttttctggta
     4561 ttaacattgg caaactgctt tcctctaata taatcttaat caccctttat atactttaat
     4621 ttattaggta tattcatttt tttcatagag atattttctt cagtttttat gcttattttc
     4681 attggataat tggtttgtat gctatctaaa tatttactac ctttatctac ataattagat
     4741 agtttctcct tatctccaat gcactttatt tcatatggtt gtgctacagg aactccattt
     4801 atatttatat ggcttcctgc aagtacaact tctgaatatt gattaattct ttgaccattt
     4861 atttctatgt attttcctcc gtttactttt aattcattta taacttttac aagaatctta
     4921 ttatagtcta tactattagc tatattccct gttttttcat ctatagcatc tatacttaat
     4981 attatcccac taccctttac atctgaatat gatagactat ctctaagtgt ttctatttct
     5041 gatatttctt cagaattttt ttgattttgt ttcattgaat taatttcttt atctaacttg
     5101 tctttctctt tggttaattc ttttatagat tttcttgtca tttttatttc ttttcttatt
     5161 gacttgtctt tgcttgtata aactgtttct tcattgctat aatctctaaa tatggttcca
     5221 acaagtatac ctaaaatagt acttgttagt attataaaca catattgttt tttcatactg
     5281 tattgctcct tacaaattta tttttatatg ataattttct ttaattaaat attaaacatt
     5341 tacatatgct tattatatca aagttatata ttaaaattca tctttagtat ataatatcat
     5401 acattgaact cctatcacta atttaccaat atcaagtatg catttttaga tgttcttaaa
     5461 attatataaa ctttcaataa acattgtttt gatattataa aaaaatataa attaggaaag
     5521 gaaataataa ttatgaaaaa caaataattg ctttggagat tattaatatc attttttagc
     5581 aaaaaaaagt aaaatggcat agcctcaaag ctacaccaat ttactaaaaa ttatatcatt
     5641 attaacatta attttaaaca tatacgttta taagttttcc tcatatttta aaattctttt
     5701 aattaattta tctcttaatt tccctacttc atcaaatctc atattaggac tatagtaact
     5761 ttctataata tcatgtttag acttggctct cttaagattt agtactgccc aacaaattaa
     5821 atcatctaaa actttttcat caaatttaat atcttcttca tatttaagta gtttttctct
     5881 atttaaaaac tgatttaaat caattgtatc ttcttcttta aaagtcgtac ttatagttat
     5941 acctatgtct aaatcagtta atagtacagt ttcaattttc tctggaataa gtggataatg
     6001 ataaacttct acatttaaac ctttttcaat agctttctta tatactttgt ttaataatgt
     6061 agttttccca gttcctattt ctccatctaa ataatagact ttctttacat cttttaaaat
     6121 gttttctgta taatctaaac accctacagg agttatagct gaaccaaata aatgcttttc
     6181 tctttttaga tttcctgtgc ttttaactcc atcaaataac ctttttataa attcttctgt
     6241 gaccaaattt actttgccat aatccataca atcagaatat ttttcttcta tgtctctaaa
     6301 gattggttct gctgatttta aaaacttaaa tgctctttta aatgaattac taatatcttt
     6361 atcataaact gatatttctt gtttattctt ttctaattct tctgtattcc agtaatcacc
     6421 taaattaatt atctcatcca aagctccagg tagctttggg tccatagtat gtggggcagt
     6481 tgcatccata agtaccacgc ctaatttctt tataactaaa ccatccaacg aacttgggtc
     6541 agaaggacaa tgatataaat caatatcata tcctctatgc aaaaactctt tataaatttt
     6601 tttcataagt gaggattttc ctactccagg tccacccttt aaacaaaaaa ctctttttac
     6661 attttcagga attatatagt caaaaaaaga atatgagcct tgtgatgtat ttccacccgg
     6721 gaatgccttc tttacctttc cttccatatt agcgcctcct cataatttac aatatctctt
     6781 aattaagata ttcatattat aagaatttta ttacagcttt cttattttga caaatttaaa
     6841 acttgccatc taatactaat aacaggttaa ttttatctaa ttattataga ttctcatact
     6901 gtgccttatt ctatctataa atacaattta agtgtccata ttgaaatatt tgtattgtaa
     6961 tacagctgga tattacttaa atccaattgt ttccattata attttatgtt aaaataatat
     7021 tacaaaatac atctgttttt cttcataaac gggtgaaatt ccctatcggc ggtaaaagcc
     7081 cgcgagcctt atggcataat ttggtcatat tccaaagcca acagtaaaat ctggatggta
     7141 gaagaaaata gtatatgagt acctttatgt aattttacat gagtaatcta tacaaatcct
     7201 tcaactaccg tatttattca tgaaattaga cacattcaag gtacctaata tacaggtgct
     7261 ttttttgttg tttattttac aattatatcg tacttataaa atctattaag attggagtgt
     7321 tatcatgaaa caaaaatgga tagtattgat tatcatctgt attggtgtat ttatgtctac
     7381 tcttgatgga agtatactaa atatcgcaaa tcctacaatt gctgctgact ttaaaattaa
     7441 catgtcacaa attcagtggg ttgtaacagc ttatatgctt gttgtaacag ctactatgtt
     7501 atttttcgga aaacttggcg ataaagtagg ttcaaatcgt ctatacacat taggattttt
     7561 tatatttaca ataggttctt ttctgtgtag tatgtcaaac aatttatcaa cattaatttc
     7621 ttcaagaatc tttcaggcag ttggagcaag tattttaatg gctactgggc ttggtatagt
     7681 ttccaatgca tttccagcaa atgaaaaagg aaaagctata ggaatcactg gagctgtagt
     7741 tggtatagga aatatgagtg gtcctgtaat tggaggtata attttagaac attttggatg
     7801 gccttctatt tttattatca atatccctat tggtattata gcagtctttc ttggcattaa
     7861 atttctgcct aaacctgttt tggatgaaca aaacaagagc tttgatattc caggtcttct
     7921 acttttcgca tcttgcacta cgcttatatt acttgcaatg aatgagaagg gtaatacaag
     7981 attgtattta ggtataacag cacttataat cttcttattg ttggcattaa gagaagtaaa
     8041 atttgaacaa agctttattg acctacctct atttaaaaat agaaacttta ctgtaggcaa
     8101 tatcattgga gtagcttgtt attttcctca aatggcagta tcttttctac ttccatttta
     8161 tttagaacaa ttaaaaaatt tatctcctat gatggctggt tatgtaatga ctgtacatcc
     8221 acttattatg gtacttatag cacctattgc aggttcactt tctgataaac acggtgcaaa
     8281 aaacattcta acagcatctt tttcatttat gaccatctca ctagttggaa tggcactttt
     8341 aaaagcagat tctccattgt atcttttaat tgtatgttta gttatttttg gacttgggct
     8401 aggagcattt agttcaccaa acaatagtag tattttagca gatgttcctc ctcaaaaaca
     8461 gggatacgga ggcagttttc ttgcaactat tcgtaattta tcatttgctc ttggaacagc
     8521 attttttagt agtttttttg cacaaagtct tacatacaat caaaagttta aaagtcacac
     8581 atcagcctat gttatagcta gcaatcagtc ttattggatt gcagcatcag tatgttttat
     8641 agggcttata ctaactgttt tctttatgag aaaaacagat aaatctattt cttaaatgct
     8701 atttattgct gactacataa gtcaataatt ttttagttga actaatttat actaattttc
     8761 ccatacctta ctaaaattta ttagtattta gcaaaattac tgatatttat tgtttataaa
     8821 caataaggat gccaaaaaaa gttcattaaa actattttaa ggcatcctat attttgcttt
     8881 aatattaata taattaaaac taactattat tacatttatt attcaaataa atcttatgtc
     8941 aatatttaac attttaaatt taatctactg ttatttcata tccatgttga ttgtattgat
     9001 ttacaatctc ttcccataat tcttcaacac cagtcttttt tagtgaagaa acaggaatta
     9061 ctttttcact actttccatt tgcaattttt ttcttattat acttatgtgt ttttgatatt
     9121 gtcctctaga tattttatct gccttagttg caataactac acagttatat ccaaagtgct
     9181 ttatccattc atacataagc ttatcatctg ctgtaggctc atgtctaata tctacaagta
     9241 ggaatataga acataactct tgtctacttt ctagatatcg ttccattata cctccccact
     9301 tttctttttc agacttagct atctttgcat atccatagcc tggaaggtct acaaaataaa
     9361 attcattatt tattagataa aaatttatag ttctagtttt tcctggtgtt tgactggttc
     9421 tagcaaagtt tcttctgtta agcaatgtat ttattattga tgattttcca acatttgacc
     9481 taccagccaa tgctatctct gggattcctt ctgctggata ctgtgattta ttcacagcac
     9541 tcatagttat ctctgaactt ctaattttca ttattttcac cactctttaa taaagcttgt
     9601 tctaatactt catccatatg ttctacaagt acaaactcca ttttttcttt tacattttct
     9661 ggtatttcat ctaaatcagc ttcacattct ttaggaatta atacttttgt tataccagct
     9721 ctatgagctg ctaataactt ttctttaact cctcctactg ctaagactct acctcttaaa
     9781 gttatctctc ctgtcattgc tatatttcca ggcacaggtc ttttagtaag tgctgagatt
     9841 actgcaagag ccatagttat accagctgat ggtccatctt ttggaacagc accttctgga
     9901 atgtgtatat gaatatcatt cgtcttataa aattcagggt ctatatcaaa cttgtccact
     9961 attgacctta tataagagat acctgtcttc gcagattcct tcataacatc tcctaatttt
    10021 ccagttaata caatttcgcc tttaccttta agtacgttta cttctacttc aagtgtaact
    10081 ccaccaactt ctgtccacgc aagaccattt actaatccta tttgagggtt aacttctgca
    10141 agttgatact taaacatgtc ttttccaaga tatgtctcta ggtcagactt atttataaca
    10201 acctcttcaa gagttgggtc ttcaacgtat tttttggcaa cctttctaca aatcttacca
    10261 atagttcttt ctagtgttct tacacctgct tctcttgtat aatggttgat tatacttctt
    10321 aaagtttcat catctatttt tataaagttt tcttttaaag catgttcttt aatctgtttt
    10381 ggaagtaggt atttttttgc tatatttaat ttttcttctt ctatatatcc agatacttct
    10441 attacttcca ttctatctaa taatggtctt ggtatattac ctagactatt tgctgttgta
    10501 acaaatagta tttttgataa atcaaaaggt atttctaagt aatggtccac aaaatcctta
    10561 ttttgctctg ggtctaatac ttctagcatt gctgaagctg ggtcaccttt ataatctgct
    10621 gccatcttat ctatctcatc aaataagaat acaggatttt ttgtttgggc ttcttttacc
    10681 ccattaatta ttcttcctgg aattgaacct acataagttc ttctatgacc tcttatttct
    10741 gcttcatctc taactccacc caaagatatt cttacaaatt ttctatttaa tgctctggcg
    10801 attgatttta ctatagatgt ctttcctgtt cctggaggac caactagaca tattataggc
    10861 cctttaagtg acttagcaag agttcttata gctagatact ctagtattct ctccttaact
    10921 ttttctaatc cataatggtc ttcatctaat atatcttttg ccttagttat atctaattta
    10981 tcttttgtct ctttattcca tggcaatgag aatatagtat ctaaatagtt tctacttaca
    11041 gatacatctg gagacataga agatattttt gaaaatttat ctatctcttt ttctattttc
    11101 tctttagttg tcttaggtgc ttttattttt ttaagctttt ctctatactc atctgcttca
    11161 gagtttatat cttcttcttc acctaattct ttctgtatag cttttaattg ttctctaaga
    11221 taatattctt tttgaacctt attcatctgt ttttttactc ttaaagttat tttcttttct
    11281 atttttaata tatctatttc ctctaataat atagaataaa taagttcaag tctttttcta
    11341 atatcaaatt cttctaatat ttcttgtttt tggcttgatt ttagatatat gtttgcagct
    11401 atcgtatcta taaatctatc tacatcttct atatcagcta aagatattaa tatttctggt
    11461 gataccctat ttccaatatt tatatactct tcaaaggcat caaatacatt tcttacaaat
    11521 gcttcaattt caacttctgt ttcactatcc aaattatcac tatcaaatac tatttcttct
    11581 attactgctc tgaaataccc atcttcttgt tctattttct ttactctacc ccttgataca
    11641 ccttcaacca aaacacgaac tgtatctcct ggtaatttta tcatttgctt aactttacag
    11701 attgttccca catgataaaa atcctcttct cctggctcat ctacctctgc ttctttctga
    11761 gaagttaaaa atataagttc ttcatccatc atagcttggt ctaaagcttt aagagatatc
    11821 tctctaccta tatcaaaatt taatatcata tatggaaata tagctaatcc tcttagagga
    11881 attaatggta attcatgatc tattttagta taattttgtt ccatcaacat tactcctttt
    11941 ctagtctaat tgctttgctt ttaatattat atatttgaac aagttaattt tcaacattaa
    12001 tgataaattc ttagaatact atcatactat attatctttt tattatgata taatattaat
    12061 attagtatac ttagatgtta tttatctttt ggaggtttct attatgttga aattgttttt
    12121 aaccttttta aaaattggtg cgtttacatt tggtggtgga tacgctatgg ttcctcttat
    12181 acaagatgaa cttgtaaaca aacaaaaact tttagagcag gaagaattta ttgattattt
    12241 atccatagct caaagttatc ctggagtact ggcagttaat atatctgtat tattaggata
    12301 taaactttat ggtataccag gagttcttat atgtacctta ggctctgctt taccatcatt
    12361 ttgtatagtc ttagtcttat cgtatctata ctttaaaaat tcatcatcta aaattttaga
    12421 tggatttttt aaaggagttg cgcctgttgt aatagctctt atattgtatt cttttacgag
    12481 catgttcaag aaactaccaa agtctaaaac taacatacta ctattaatta tagcagtttt
    12541 atctgttggt gtattcaata taagccctat ttatttaata attggaggag gtgtatatag
    12601 tctatgtcag aaataatact actcttctta atattcctta aaataggggc atttagtttt
    12661 ggtggcggtt atgccatgct accatttatc cagcaagaat ttatatctaa atatcacttt
    12721 ataactaatc aagagttttt ggatttactt gctatatcac aatctacacc aggtcctgta
    12781 gcaataaata gtgctacatt tgtaggattt aagactgctg gcttctttgg ttctctagct
    12841 gcaactattg gtgttacaat ttttgcatta ataggtctaa ctattatatc aaagttattt
    12901 tctaaattta aaaataataa aaaggtagaa aacttattga cagttcttag accaattact
    12961 ctaggattca tattatcagc agctttttca tctgccaaag aagttaattg ggatttatat
    13021 ggaataattg catttatagc atcattttat cttttatata aaggaaaaat tggttccatt
    13081 gcaattgtat tcgtctttgg tattctaggt atattactca catttttata ataatgatac
    13141 ctattttata tttagcatat taacataaaa aaaggagctt ttaaagctcc ttttcatcat
    13201 cttatgcact ttcctcttga tccttaagta ctatgatagg atttactgag tcttcgttca
    13261 cagatttctc tgttactata accttcttta tattatctct agatggtact tcaaacatag
    13321 tttccatcat tacgctttca actatacttc taagtcctct agctcctgta tttctttcta
    13381 tggccttttt agcgattgca cgaagagcac cttcttcaaa ttctagctca acatcatcta
    13441 actccaataa ctttttatac tgttttacta gagcattttt aggttcctgt aatatctgca
    13501 ttaatgcatc ttcatccaac aactctaaag tagctaaaac tggtattctt cctataaact
    13561 ctggtattat accatatttt aataaatcct ctggaagtac tttttcatat agcttcccta
    13621 aatctaattc ttttttactt tctattttag ctccaaaacc tagtgtttta tctccaccac
    13681 gtttttgaat tatcttctct aatccatcaa aagctccacc taatataaat aatacattag
    13741 tagtatctat ctttaaaaat tcttgatgag gatgttttct tcctccttgt ggtggaacat
    13801 ttgcaacagt accttctaat atttttagaa gtgcttgttg aacaccttca ccactaacat
    13861 ctctagttat agatgggttt tcagattttc ttgcaatctt atctatctca tctatatata
    13921 ttatacctct ttcagctttt tctatatcaa aatcagcagc ttgaataagt tttaaaagta
    13981 tattttcaac gtcttctcca acatatccag cttcagtcaa tgatgtagca tctgccattg
    14041 caaaaggtac atttagagtt cttgctagag tttgagcaag taatgtcttt ccagaacctg
    14101 taggtcctaa taaaagtata ttactctttt gaatttctat atccttacta ctagattttt
    14161 tactgtatat tctcttataa tgattataaa ccgcaactga taaagctttt ttagcttttt
    14221 cttgacctat tacataatca tttagaatct ccatcatttc ctttggtttt ggtaaagatg
    14281 tagtatcctc atctatagta tcttctatct cttcttgaat aatttcgtca caaagttcaa
    14341 cacactcgtc acaaatatat acatttggac ctgcaattaa tcttcttact tgatcttgat
    14401 ttttaccgca gaatgaacac tttaattgtc gtttttcttc atatttagac atattaacac
    14461 ctctctctat ctacggtctc tttgtaaata cttcatctat taaaccatat tctttggctt
    14521 ctagtgcact catgaagtta tcacgttcag tatccatttt aattttttct aatggctgac
    14581 cagttctttc agataatatc tcatttaaag tttcttttat ttttaaaatt ctctttgcat
    14641 gaatttctat atctgttgct tgaccttgag ctccacctaa tggttgatgt atcattattt
    14701 cactatttgg tagtgctagt ctttttcctt ttgctcctgc tgccaacaag aatgctccca
    14761 tagaagcagc catacctata catatagtac ttacatctgg tttgatgtat tgcatagtat
    14821 catatatagc cattccagaa gttatacttc ctcctggaga atttatatat aaatgtatat
    14881 ctttgtctgg gtcttcagct tctaaaaata atagctgtgc tactataagc cctgctgtgg
    14941 catcgttaac ttgatctcct aagaagatta ttctgtcttt taatagtcta gaaaaaatat
    15001 cataagatct ttctcctctt cctgtttgtt caacaactac aggtactaat gccatggttt
    15061 cataccccct ttattctata taatttacag tagctttttt taagctactg taaattatta
    15121 tacgcattta actaaattat atttgcacat cattatatac taaacatttc ataacgtcaa
    15181 ctatattagt tagcgtttat tttattaagc tattgttgca ttatcaacta ataaatctat
    15241 tgtttttctt atttttattt gaccttttat atcttctttg tcagcatctc ttaaagaagc
    15301 ttctattttt tctacttcca tattgtaagc agaagccatt ttttctaact cagccttaaa
    15361 ttcttcttct gttgcttcaa ttccttcagc tttagttatt gcttctaata ctaaagaaga
    15421 cttaactaat tttttagcat cttcttttct ctcttctctt aattcttcca tagttctacc
    15481 agtcatctct agtaattgtt gtaatccaaa tccttgatat tgtaattggt agttaagttc
    15541 atttaacata ttatctattt gatgttgtat cataacttct ggtatttcta tttctgtatt
    15601 ttcagcaact ttttctacta ctgaatttct agtttctgca tctgctctat ttttagcttc
    15661 ttcttctaac ttagctctta catctgcttt taattcatct aaagaatcaa attcactagt
    15721 atctttagca aattcatcat caagtgcaga taattctttt acttttacat catttatttt
    15781 tacattaaat acaactggtt ttccagctaa atcttgtgag tgatattctt ctgggaaagt
    15841 aacattaact tctacttctt caccagcttt tttacctact aattgttctt caaatcctgg
    15901 tataaatgtg tttgaaccta taactaagtt ataattttca cctttaccac catcaaaagc
    15961 tacaccattt tcaaaacctt cgaagtctat tatagctgta tctccatctt ctaaagcttt
    16021 gtcttctaca cttactaatc tggcattttt ttcaaccatt tcttgtagtt tagcatcaac
    16081 attttcgtca cttacagtat tctcaacctt agctatttct atacccttgt agtttcctaa
    16141 ttcaaactct ggtttaactt ctacattaag aaccataacc aacccattgt ctttacttat
    16201 ttcttctata tctaaatctg gattgtctat tgggtctatg tctaattcct tgatagcttc
    16261 tggataaact tctgggaata atatttctat agcatcattg tagaatatac cttttccgta
    16321 ttgagtctct ataacaattc ttggagcttt accttttcta aatccaggta tattgtattt
    16381 atttctgctt ttattataag ccttatttac tgctgcttca aatttattgt tatctactgt
    16441 tatttttaaa gtaactttat taccctcttt tttaattagt tctgctttca tccttctaat
    16501 cctcctagtg atatttatgt aatacccgat aacggtatta taattcattc tatttataat
    16561 tcaaccactt aattatatca cataagtttt tttatttaca agtgttccat taatatcgtt
    16621 gtacttattt gataaaattt aattttttga gcgttttttg ccattttttt agataaatac
    16681 acctttatat gcgtatttct tcaattttta tgtcatcttc ttctatatcc ataatgacaa
    16741 aacttttata cgatactcct cttggaagtg aagtgcttcc aggatttatg taaagtacac
    16801 catctttaat ttctctaaat ggggtatggg tatggccaaa tataactata tcagcatcaa
    16861 cttcagttgc ctttttttct aacatattag ttccatattt tacaccatac ttatctccat
    16921 ggcataagaa tatggtttta tttgccactt caaagacaac ttcttcctca acagcatcaa
    16981 agtcacaatt acctttaact gcaataatac ctacattagt catgctatga atatatcttg
    17041 aatcagtaaa gttatctcct gcatgaatta ttaggtcaca ttctttcaaa tatggtattg
    17101 ttttttccat atttttaatc atcatatgtg tgtcgcttac tatgcctatt ttcattactt
    17161 cactaccctt tttataaact cttgtttcat aagttttagt gcgtttgctc tatgactgat
    17221 tgaatttttt ataacactag gtatttcacc aaaagtctta tcatatcctt ttactataaa
    17281 taagctatca tatccaaaac catttttacc tttttcttca aatcctatca ttccttcaca
    17341 aattcctctt actacaaatt cttttccatc aggaaaaact actgctattg cagagacgaa
    17401 tcttgcattt cttttgctca ttggtatatt ttgcatagct tttaacagtt tttttctatt
    17461 ttcttcatct gttgcatgtt ctcctgcata tctagctgaa taaacaccag gttttttgcc
    17521 taaagcatct acttctaagc cagaatcatc tgatattgct ataagtttag tcttttttgc
    17581 tattgttcta gcttttatca acgcattatg ctcaaatgtt tttccatctt ctactatctc
    17641 tatacctgct aaatcaacat cttttaaaga gtacacttta tattcaaaat cttttaagat
    17701 ttcacctatt tcttctagct tatgagcatt attagtagca atcactactt cattaccata
    17761 atccataccc aatatctcat ctgatatttc acctaaagct tctctttgaa gccttattaa
    17821 ttctttattc cctttttcac ctaactctaa caataaattt aaatctgctc tcgaaaaagg
    17881 tttctcttct cctgtcccct gtacttctac aaactcacac ttatcagtca ttattatatt
    17941 catatctacc tgtgctttag aatcttcttc ataacataaa tctagcatgt gtattccatt
    18001 tacaatacca acactaattg cagacacaaa atttgttatt ggcatctctt ttattgtctt
    18061 tgctttatat aatttatata ttgcttctgc aactgctaca aatgcccctg ttatagatgc
    18121 agttctagtt ccaccatctg cctgaatcac atcacaatct acccaaatag ttctttctcc
    18181 taatttattc aagtctacaa cagaccttat tgctcttcct attaacctct gtatttcttg
    18241 tgttcttcca tcaatttttc ccctagatgc atctcttatc tttctttgtt gtgttgctct
    18301 tggtagcata gaatattcag catttatcca tccagtacca ctatttctta aaaaaggagg
    18361 aactttttct tccactgatg cagtgcatat aacctttgtc tctcccattt ctataagaac
    18421 agaaccttct gcatactttg tataatctct agttatcttt acaggtctta tctgttcatt
    18481 acttctatca taacttctgg tcatttttta tatcctttct catatttttt attttttata
    18541 aatttatttt tataattttt aattttgtca aatattaatt attgtcattt ttcgtaatct
    18601 tattagctta ctgaactatt ctatcattaa ttatagtagt tttctactaa attaagcgac
    18661 tttttaactc tctaatacag cttttacttc atctttcgat gataacttta ataattctaa
    18721 aaatctctct tcatctataa ctttaattcc taaatcattt gctttagtaa gtttagaacc
    18781 agcttcttca cctgccaaaa caaatgttgt cgatttacta acactagatg tagcttttcc
    18841 acctcttttt tctatcatat cttttgcatc atttcttttt aatgtaggaa gtgtaccagt
    18901 caatacgatt ttcatccctt caaatatttt ttctactttt tcacctgtat tatccaatga
    18961 ttttgtattt actcctacat tttttaactt gtttataacg ttcatatttt tgtcttcctt
    19021 aaaaaactcg acaacgctgt ttgccattat acttccaaat tcctctaatt cttctagttg
    19081 ctcactagtt gcagaaatta cttcatctaa atccttaaag ttatatgcaa gtatcttagc
    19141 tgctttaact cctataaatt taatacctaa tccatttata agtctccata aatcattttc
    19201 ttttgatttt tcaattgaat ttattaaatt gtcagcagat ttttcgccca ttctctctag
    19261 gtcgactaca tcttcctttt ttatataata taaatcagat acatcttgaa taattttttc
    19321 atttaatagc agtgtaatta tagattctcc caatccatct atattcattg catcccttga
    19381 cacaaagtgt ataattcccc ttcttatttg ggctgggcaa gacatattga tacattttac
    19441 agcagcctct ccttctaatc ttattgttgg ctctgaacaa actggacact tttctggcat
    19501 tttaaaatct atttcatctc cagtacgttc ttcttttata acttctaaaa cttgaggtat
    19561 tatgtcccct gctttttgaa ctaatactgt atcatttatt tttatatctt tttctcttat
    19621 ataatcttca ttgtgaagtg tagctctact tacagtagta cctgcaagtc tgactggttc
    19681 aagaattgct gaaggtgtta ttgtaccagt tctcccaact tctactatta tatctactat
    19741 tttagttttc ttcttttctg ctgggaattt atatgcaatt gcccatctag gacttttggc
    19801 tgtatatccc atttcgtctc tttgttttat attgtttact tttattacca taccatctat
    19861 ttcgtagtct aaattacccc tgttatctgt ccagtattca atatgctcaa taacttcatc
    19921 tatactttta caaactttgt aactctgact tactgaaaat cctagttttt ctaagtactc
    19981 tagtgattca ctgtgacttt ctaaatcgta ttcttctata ttttccatat taaatacaaa
    20041 tatatctaat ggtctctttg ctgttagttt tgggtctaat tgtctcaatg aaccagctgc
    20101 aagattcctt ggattagcaa ataatggctg ttcttgttct tcttgttgtt catttacttt
    20161 tctaaaatta tcttttgata tatatacttc tcctctaact attagttctt ttttatcatt
    20221 tatcttaaga ggtatgcttt ttactgtcat taagttttga gaaatatctt ctcctacaac
    20281 tccatctcct ctagttgctc ctttttcaaa ctctccattg ttataagtta gacctacaga
    20341 aagaccatct attttaaatt caacaacata ttcaacattt tctcccacat attcttgaac
    20401 tctcttatca aaatctctca aatcttgctc tgaaaaagca tttgataaac tcaacatagg
    20461 agttttatgt acaatttgat tgaacttatc taatggctta cctcctaccc tatttgttgg
    20521 agaatcaatt ctctttagtt ctggatgttc ttcttctaaa gaaattaact ccttcattaa
    20581 cttatcgtat tcataatctg atatttctgg gctatcttga ttataatacc tttcattatg
    20641 gtagtttatt gtctctataa gtttattaat ttcatctttt acgctcactt tttgcacctc
    20701 ttcatatttt taaaattaaa ttattttgaa atttccctat aattaattat ctcctattta
    20761 tatatttttt agtatatcac ttttttggct ttaaatttat tacaaaaaaa actcttatta
    20821 gtatctttaa tacgactaat aagagttttt atttttatca aaatttttta tttttaatag
    20881 cctcttgata tgtctacaac attatctaca ggtttattct ccattatata gtgtttcaaa
    20941 ttattataaa ccatattaaa tgttctctct ttgtttttat ctgacaccca agaattatga
    21001 ggagttacta tgacattttc gcactcccac aacttattgt ccttgtttaa aggttcactt
    21061 tcaaatacgt ctaatgctac ccctctaaat ttctctacac attcttctaa atctttctga
    21121 ttaactgtat ttcctctacc tacatttata aatacagaac catctttcat aagtttaaac
    21181 atatctttgt ttactattcc atctgtactt tttgtagaag gcattgctac aactactata
    21241 tcacattctt caaatacaat attcatatcc tcagtagcaa aacacttatc aaagtatttt
    21301 ttactacttc catcagtatt gacaccccat atttctacac caaaagcttt taatcttttt
    21361 gctgcttctg aagatatagt acctgttcct aaaaatccaa ctctttgtcc atacaattct
    21421 gatacagaaa aatccatctt ccattcttta ttttgttgtt gtttaaaaaa ttttgcacta
    21481 tttttatata tttctaaaat atacaataca atactttcac caataggtac actatatcca
    21541 cctctattat tagctatctt aatatttctc ttcaatacct cttcttttgg aagttggtct
    21601 atacctacac tagttgtctg tatatattgt aaatttttca tcttagaaat atctaatctc
    21661 tcaaatggat tgtatgtaac tagtacatct gctgtattta catcctcatt attttcaata
    21721 ctatcttcag agtaatgtat gacatcatag cctaattctc ttattttatc aaatttttct
    21781 tttccataat ttattgtaaa taaaactttc atctcaaatc tcctccatct acccacattt
    21841 aaaattttat attctaaaat ataatatgat actcaatgta cattagttaa aattactata
    21901 agatatctta aatttactta tgttcaattg tttttcctaa agacatagca ttacgttctt
    21961 gaactaaatt ttctattaaa ttattcaatg tccaatcaat agatgtctct gttaccatag
    22021 ctgtaaaagg tctatttatt ctcacttttt ttagcccttc taaaaatgca tttacttgtg
    22081 tgcttggagt gttattaaat attacagact tactcttaat cccattttca cattcatttg
    22141 atatatttcc atctaaaatg tctcttatta tattctctcc atttttatca cttaagatga
    22201 tatggtctct tattccagca aagttactta cgtttttaat taatgataat tcttttttat
    22261 taaagtttac aagcataata cacttcctgt tgtgtatttt atctaggtta ttattattta
    22321 ttttttcaaa tgacatttta tccatccttt catttattta tatactttaa tttaccccat
    22381 acatttaaaa taatcaattt ttatagttaa tttttatatt aatacccaaa ttatatttaa
    22441 atagttaggc tatccaaaac taatatataa aattagaata attagattaa tatctacaaa
    22501 tcataaccta aaggagagat accatgaata cattactaaa caataaattt agcattaaaa
    22561 acttttcaaa tgcttgggtt atagtacttg gttgtatgtt gatacaagct gttccattta
    22621 gtattgtagc aagtatacag cctcaattta taagttatgt tgtagaagga gaaggcttta
    22681 gtttagcttc tttttctcta atattcacta ttggtactat tgcctcagca attgcatcac
    22741 cttttatagg tgtattattt tctaaagtca atattaagat actatattta ttaggttgta
    22801 taatttctag tattgctttt gctacttttt ctatatgtac tgaactatgg caattttaca
    22861 ctgtatcatg tattgttcaa attggcgcta caattatatc tgctatagga atacctgttt
    22921 taataagtgc ttggtttgat gaagaaagca aaggaaaagc tttgggcttt gcttttgctg
    22981 gtggttctat tggtagtata tttctacaac cacttgcagt tcgcttatta gcaactcaag
    23041 gatacagtcg ttcttatcta atatttggaa ttatagcatt aatagttggt atacctgtaa
    23101 tattatttat actcaggatg cctaaagata ttaatgaaaa ggtaaaatct aaaggtaaaa
    23161 aagagaaagc atctgataca atttctcctt cagtatgggg ttatacatta aaagaagcta
    23221 aaaaaattaa atatttttgg attatgacta ttggatttat atttgttggt atatatgttt
    23281 catcattatc tgtacaatat ccaacttatc ttagaaatta tctaaatcct attctaatag
    23341 gaacagtagg ctccacattt gctttatttg ctctcattgg taatttattt ggaggattat
    23401 tgtttgataa attcggtcaa acaaagccac ttattatatc ttttgtattg gttcttatag
    23461 caactttatc attactattg gtagataaat cttctacttt tgcatttgta tttgccattt
    23521 tgaaaggtct ttctgtattt gcttatatga ttggtcctgc ttatttaact ggctctttct
    23581 ttggtcaaaa ggagtttggt agcatatatg gaatcgtaca attaattttt gcagtgggat
    23641 ttgcattagg ttcaacatta tttggattgt tagttgataa ttttggatac agattttctt
    23701 ggtctattat aattttatca gttataattg cctatacttt acttattata acttctatag
    23761 gaatgaaaaa tttgaataaa gaaagattag ataaactaat gcaataattg atttttagat
    23821 atataaatat tataataaaa aagatggagc ttttaatcat aaataaaact ccatcttttt
    23881 tatattacaa tatagctaat tttctcttat ttttaacttt actatctata tcctcatcct
    23941 taactatagt aaatgcctta gttggacaaa catttacaca tgcaggccct tctggcgatt
    24001 ctatacaaaa atcacactta tttgctgtca ttctttctct aaagcaaagc tttccttcat
    24061 ctacatcttt taatgtaact atgtctattg cacctatagg acatgctaac atacaagtct
    24121 tacatcctat acaattttct tcattgattt ttattgtgtt accaacccta actataccac
    24181 catttggaca tgcctttgca caaggagcat cttcacaatg tctacactgt attggagcac
    24241 ttacttcttt tgtctttatg acagaaagtt ttggattaaa atttatttca tctggatttt
    24301 ggtaaaaaat atcttcatct gaatgtgcaa ctacacatcc tatcatacaa gttctacatc
    24361 ctatgcattt atcagggtct gctataacaa aatagttcat cacttagcca cttcctttcc
    24421 tatacaatat gtagtgtgta atagatgatg agatttttct cctaatggtt ctaccagata
    24481 ttcatcatac agttttttaa tatcaggatt ctcatgagat tttctaattg ctaaatgttc
    24541 atcatgcacg tatgttgctt ttgttctatt ttcataagcc aattccttat attcttctag
    24601 taaaattttt ggttgacctc caccacttac acacccaaca ggacaagtca taacttcaat
    24661 aaaatctaca tctaattttc ctttttccaa gtcttcaagc actggtatta cattttttaa
    24721 tccacttact actccaactc ttaagtctaa gtctcctatt ttaatagttg actttctaaa
    24781 accattttct cctcttactt gttttacttc tacatcagga atactttctc cagttataag
    24841 ctcatatcct gttcttatag cagcttccat aaccccacca gtaacaccaa agatagttcc
    24901 agctccacta taactaccta aaggactatc aaatttttct tctcttaaat ttttaaaatc
    24961 aattcccatg tcttttatta aataagctag ttctctagta gttaatacta catctacatc
    25021 tctatatcca ctagaatcca tttcttctct atcacattca tattttttac atgtacatgg
    25081 cattatagaa acactaaata tctttgatgc atctacatta tttatctctg caccataagt
    25141 tttaaatatt gcaccaccca tctgctgagg tgatttacaa gaagataaat gattagttaa
    25201 tttaggataa tttttttcca tgaattttac ccaagctgga caacaagatg taaacattgg
    25261 aagagttcca ccttctgtca ctcttttaat taattcacta ccttcttcca ttatagttaa
    25321 atctgctgca aaattagtat cgtagactct atcaaaattt aaagctctaa gtgctgctgc
    25381 cattttccca gcacctaagt atcctaaata atatccaaaa tcttctccta aagcaactct
    25441 aactgctggt gcacattgga ccatagtgaa tacatttggg tcacttaaag ctttcttcac
    25501 cttatctata tcacatacat tgtatgctgc aaatataggt tcatttatag actctggtat
    25561 ctctctttct tgttttttct cttgtaaaaa ctcaaatcct tcatctataa ctgaggcata
    25621 agatttacaa gtttgaacac attgaccgca cataacgcat ctatctaaat ttatctcttg
    25681 tggctttccc tcttcacctt caatagcatt tactggacag acctttgaac attctctaca
    25741 tccagtacat aaatccttat ctatatttat tatcataaac gtcacctacc cctgtatcct
    25801 ttatttattt gttaacaata aactaagtgc agcttgtcta ttcttagctt ttttgtcatc
    25861 tattggagta acaagtttta atgcttcctg aggacatgca cttatacaag ctattttttc
    25921 attgtcttta cataagtcac atttataagc tatcttctta ttttcatcta aaattacttg
    25981 ttctacttct ttaccatctt catattgtgg aagtaagtct atagctccaa aaggacatgc
    26041 tagcagacaa gttttacatc ctatacactt ttcttcattt acagacataa tattgttttc
    26101 tttaactata gctttttgtg gacatgagtt taaacaagga gcatcttcac aatgtctaca
    26161 ctgtattggc atacagaaat tgtcaccttt tactaagtaa agtctaggta taacaggtat
    26221 atcaactgtt ccaactgtat aacccacatt attatgctga ttatgcattg taaaacaagc
    26281 tacttcacaa gctctacaac ctgtacattt gtttgggtct gctattacaa aagagcctat
    26341 tttatttttc atattatccc acctttcaat ttgtatattt ccaaccatct agttattttc
    26401 atcatatgtc tacttacttt catcatatat atttatatat ttcactattt tctcagtact
    26461 tatatttcgt ttacattttt tgcacaaatt gtatgtgtta ttatacacct tatcagtatc
    26521 atcaattaat actccacaga tttcacactt tgaatatctt tgcttttctt ttttagtaca
    26581 catatattca ttctcacttt ttgataactc tctgtggatt tgcataaata ttcatccgtc
    26641 ttcctcttac aaatccaata agcgttatat ttaatttttt agaaagctct attgataaat
    26701 tagtcggtgc tgattttgat actacaatag gaatttgagt catagctgct tttaatatca
    26761 tttccaatga tattcttcca cttacagcca caattttatc ttttaatgtt atgtcattta
    26821 aaacacaaaa tccaatagcc ttatccattg cattatgcct tgctacatct tcacaagtga
    26881 ctattttttt atcaaaatta aatacagata cgctatgtac cccaccagta tttttaaata
    26941 attctgatga atttaaattt ttatccatta tactgtaaat cgtatcataa tttattttta
    27001 tattgctctt tacaggagca caggttatat agtctagtga atttaaaaat ataatttgct
    27061 catcttgatt tttttgtttc tcttgaatag ttacttttgc aaaacgttca ctctcattta
    27121 tctcaaattt taataagctt tttttgtttt ctatgtatcc ttttgttttc aagaatccta
    27181 ctataagttc ctctaaatta tatggggtac ataaaaatgt atttacatat ttatcattta
    27241 aaataaaact aagtggatat tctgcaatta tttcttcatt atctattgat acttcatgtt
    27301 catttatttt gcttacttct acattaaaag aatttaaata tttgtagtcc actagactat
    27361 aactattttc aaaaacttta tccttactat tttcttgtaa tttagttttt gagtatatat
    27421 tgttcataaa atattagcct ttctgccttt tataaatctt aaatcaaata aatcattaga
    27481 tatttgtagc tgtcattttg tctttcaaac tttgatactc ttctcttaca catttttctg
    27541 ctaattcttg gtcttcaagt ttttcaagtt taacagcaca atatttatac tctggtgttt
    27601 tagatatagg gtctaaattt gctattgtaa gttcattgca agctccaacc caccactgat
    27661 aagtcatata tgttgcacct tccttaactc tatcagtaac tgttgctctt gttattacac
    27721 ttcctcttct tgaactaatt ctaactaatt catcatcttc aattcctaat ttttcagcat
    27781 cattagaatt tatttgaaca cgtcctggtt catcttctaa tgaactaagt gttctacaat
    27841 taccagtcat tgttcttact gaatagtgtc caacttctct tacagtacat aaactaaatg
    27901 ggtattcatc atcttcaact tccatagggg gtctccattc agcagcgaac aaattacctt
    27961 taccattagg agtagaaaac ttttgacctt catacagata catagttcct ttatcttcca
    28021 tactttcact cttacaaggc cattgaacgc atccttgagt ttctatcttt tcataagtag
    28081 cacctaagaa acttggacat aattgtctaa gttcgtccca tatttcctta gtatttttat
    28141 aattcatagg atatcccatt gctgtagaaa tttcgcttat aatttgccag tctggtttta
    28201 catctccttg tggctctata gcttttctca tcaattggaa tcctctatct gcacatgtat
    28261 atactccctc atgttctccc caagaagtag ctggaagtat aacatcagca tgaagtgctg
    28321 tcttattcat aaatatatct tgaacaatga caaattccaa ttcatctaat gcttctctaa
    28381 cttctgacgc atctgggtca ctttgcactg ggtcttctcc aaatatataa taagctttaa
    28441 gctttttctc ttttagtact aagtctggaa cttgagttaa actataacca ttattaggag
    28501 ataattttac cccccaagct ttttcaaact tttctcttat cttatcatca gttacatttt
    28561 gatagccagg atatacatta ggaagagctc ccatatcaca agctccttga acattatttt
    28621 gtccacgtac aggtcctatt ccaacgcttt ctctaccaaa attacctgta agtagagcaa
    28681 ttgatgcaag tcccttaact acatcaacag cttgaccaaa ttgacagaca cccattccat
    28741 aaagtatcat agctttttta ccttttgcat attctctcat agcttttctt ataagctctt
    28801 ctgggattcc tgtaatcttt tcagcatatt tagctgtata tggtttaaca atttctttat
    28861 actcatcaaa tccttgtgta tgattttgaa caaattcttt attgtaaaga ccttcttcta
    28921 ttaatacatt tccaaaagca tttactaaaa ccatgtttgt tccacctttt ataggtagat
    28981 gtatatcagc aattcttgca gattcagtta ctctagggtc agtaacaatt aatttagcac
    29041 catttttttt agcttttact attctatttg ccactattgg atgtgaatct gctccattat
    29101 atccaaatat aaataaaaca tctgcatttt ctatctctgg tatagaatta gacattgcac
    29161 cactacctaa tgagtaagcc agaccggcta cagatggtgc atgtcagaca cgagcacaat
    29221 ggtcaacatt attagttcct attgtagctc tcataaattt ttgcattata tagtttgctt
    29281 cattaccagg tcctcttgca gaacctgttc ccataataga atcaggtcca tatttttctt
    29341 taatttcatt tagtcttgaa gctgtatagt ttatagcctc atcccattca acctcttcta
    29401 gtacaccttg ttttcttatc attggctttt ttagcctagg tgttagaatt ttagggtcat
    29461 ttagaaaatc ccatccataa cgacctttta aacacaaact ttcttgattt gttctaccat
    29521 tagccggttc agctcttaat atcttattat cttttactac tagatataat tggcatccag
    29581 ctccacagta tggacaaact gttaaaattt ttttctccat acaattcccc ccttagattt
    29641 atcccctgat aagtaaaatt tatcttaaag ataatgagtg tttttataat atgcaaaatc
    29701 ataaatattt tgcaatacta tctattaaat tcaacaattt tctcacaatt cctcttaaat
    29761 ttcaaaaaaa atatgtcata ataatcttta tatttcaacc atttaattaa aatattctag
    29821 ttcttatgac atatttttta atatactcaa tatatattct ttaaacttat ttagttttca
    29881 cttaaaacaa tatttctgcc aattctattt ttcattctat agtgagtaat tacagtaaat
    29941 attccacttc caatcagaat caaatagtac ataaatccac catataaaaa tactgcatat
    30001 cccatcaaag gttgagggaa tactgatttg aatatagtga aaaaggctaa ttcattagct
    30061 cctacattcc caggtgttgg tattggagat attgccatat ataaaaatgc ttgtaatgtc
    30121 agtatataaa tatagctata accttgtaaa ttaaatgctt tatatatcca aaatgaaaca
    30181 ctaaagtaag cacttaattg aataaaagtt actattatag ttgaaattaa taccttttta
    30241 ttctttgcaa aaaaatttat tgctttacta taatcatcta taaaactttc tatggattca
    30301 atcttattat ctaaaaattt taaaaactta atttttgata aatgctttat agtaattctc
    30361 ataaaatatt ttattttttt aggatttaaa ataactaata ttatcataat taagacaaat
    30421 gaattaatgg ctatccctag aaacactaga ggcataatca ttttcatttg tttttctaac
    30481 atagcaaaat tcattaaaac aagtattgta caataaaaag taactactat ttggaagata
    30541 atagacttat tggtaacaac tgcacttgcc ttactaagtg gcattttaca ttttttaagt
    30601 acatatatct gtactggttg gcttcctgaa gcaaaaggag ttattaaatt ataataaaat
    30661 cccataatgg ctagcttaaa accaataaat ctaatattaa ctttgtgagt gctttctata
    30721 attatcttca ttactactcc ttcaagcatt atgtgacaca ttattgccaa agcacctatg
    30781 aataaaaact tcttatctac cataaccata acatttgata gcattttaat atccaatgtt
    30841 ttaaacacta ggtaaattgt tatacctatt aacaaaaata aaaatgtata ctgtagtaaa
    30901 ctctttctaa tattctttag tctatccatt agaaataacc atcctttgct caagatttat
    30961 a
//
LOCUS       DAEOSP010000026        30647 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.026, whole genome shotgun sequence.
ACCESSION   DAEOSP010000026 DAEOSP010000000
VERSION     DAEOSP010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30647
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.026"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            122..2023
                     /locus_tag="KRH54_000952"
     CDS             122..2023
                     /locus_tag="KRH54_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG8578621.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            2026..3069
                     /gene="ypdE"
                     /locus_tag="KRH54_000953"
     CDS             2026..3069
                     /gene="ypdE"
                     /locus_tag="KRH54_000953"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG8578622.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNKKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            3084..3389
                     /locus_tag="KRH54_000954"
     CDS             3084..3389
                     /locus_tag="KRH54_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG8578623.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            3486..3821
                     /locus_tag="KRH54_000955"
     CDS             3486..3821
                     /locus_tag="KRH54_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8578624.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            3853..5175
                     /locus_tag="KRH54_000956"
     CDS             3853..5175
                     /locus_tag="KRH54_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8578625.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILSNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            5185..6255
                     /locus_tag="KRH54_000957"
     CDS             5185..6255
                     /locus_tag="KRH54_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG8578626.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIKLIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            6260..7447
                     /locus_tag="KRH54_000958"
     CDS             6260..7447
                     /locus_tag="KRH54_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG8578627.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(7644..8516)
                     /locus_tag="KRH54_000959"
     CDS             complement(7644..8516)
                     /locus_tag="KRH54_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBG8578628.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(8910..9101)
                     /locus_tag="KRH54_000960"
     CDS             complement(8910..9101)
                     /locus_tag="KRH54_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578629.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            9321..10367
                     /gene="cobT"
                     /locus_tag="KRH54_000961"
     CDS             9321..10367
                     /gene="cobT"
                     /locus_tag="KRH54_000961"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBG8578630.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            10399..10959
                     /gene="cobU"
                     /locus_tag="KRH54_000962"
     CDS             10399..10959
                     /gene="cobU"
                     /locus_tag="KRH54_000962"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBG8578631.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            10969..11742
                     /gene="cobS"
                     /locus_tag="KRH54_000963"
     CDS             10969..11742
                     /gene="cobS"
                     /locus_tag="KRH54_000963"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBG8578632.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            11762..12376
                     /locus_tag="KRH54_000964"
     CDS             11762..12376
                     /locus_tag="KRH54_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG8578633.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENNSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      12443..12622
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            12752..14251
                     /locus_tag="KRH54_000965"
     CDS             12752..14251
                     /locus_tag="KRH54_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBG8578634.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            14290..15666
                     /locus_tag="KRH54_000966"
     CDS             14290..15666
                     /locus_tag="KRH54_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBG8578635.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDASVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            15667..16638
                     /locus_tag="KRH54_000967"
     CDS             15667..16638
                     /locus_tag="KRH54_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG8578636.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQCIIRKVS
                     KNDRQLKFGGFILWLITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            16670..17740
                     /locus_tag="KRH54_000968"
     CDS             16670..17740
                     /locus_tag="KRH54_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG8578637.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            17742..18629
                     /locus_tag="KRH54_000969"
     CDS             17742..18629
                     /locus_tag="KRH54_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBG8578638.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKECLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            18617..19249
                     /locus_tag="KRH54_000970"
     CDS             18617..19249
                     /locus_tag="KRH54_000970"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBG8578639.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            19258..20484
                     /locus_tag="KRH54_000971"
     CDS             19258..20484
                     /locus_tag="KRH54_000971"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBG8578640.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            20481..21089
                     /locus_tag="KRH54_000972"
     CDS             20481..21089
                     /locus_tag="KRH54_000972"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBG8578641.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRIDNFDMNVVVILDEE"
     gene            21079..21654
                     /locus_tag="KRH54_000973"
     CDS             21079..21654
                     /locus_tag="KRH54_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBG8578642.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNIKKFNLKNIEVIKGCAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            21647..22399
                     /locus_tag="KRH54_000974"
     CDS             21647..22399
                     /locus_tag="KRH54_000974"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBG8578643.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            22383..23504
                     /gene="cbiG"
                     /locus_tag="KRH54_000975"
     CDS             22383..23504
                     /gene="cbiG"
                     /locus_tag="KRH54_000975"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBG8578644.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTQKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            23501..24226
                     /gene="cobJ"
                     /locus_tag="KRH54_000976"
     CDS             23501..24226
                     /gene="cobJ"
                     /locus_tag="KRH54_000976"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBG8578645.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            24223..24975
                     /gene="cobK"
                     /locus_tag="KRH54_000977"
     CDS             24223..24975
                     /gene="cobK"
                     /locus_tag="KRH54_000977"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBG8578646.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            24994..26472
                     /locus_tag="KRH54_000978"
     CDS             24994..26472
                     /locus_tag="KRH54_000978"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBG8578647.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            26502..27407
                     /gene="hemC"
                     /locus_tag="KRH54_000979"
     CDS             26502..27407
                     /gene="hemC"
                     /locus_tag="KRH54_000979"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBG8578648.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPIAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            27397..28896
                     /gene="cobA"
                     /locus_tag="KRH54_000980"
     CDS             27397..28896
                     /gene="cobA"
                     /locus_tag="KRH54_000980"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBG8578649.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            28913..29878
                     /gene="hemB"
                     /locus_tag="KRH54_000981"
     CDS             28913..29878
                     /gene="hemB"
                     /locus_tag="KRH54_000981"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBG8578650.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            30031..30609
                     /locus_tag="KRH54_000982"
     CDS             30031..30609
                     /locus_tag="KRH54_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBG8578651.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCRAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        12407 a         3058 c         5672 g         9510 t
ORIGIN      
        1 tgtggaatta aaatatacta ggatataaaa ttcttaaata aaaagaaaag gttgttttta
       61 taaaaaatat tttttatacc actttaaaaa acacaataat aacactgagg gaggtgggga
      121 tatgagagaa cgcattattc ttgaaacatt aaaaaatagt agtggttatc taaacataga
      181 ttattttgca gataagttgg gggtatctac tcgaactatc agaaatgaaa taaaaaagat
      241 agaaactata aaagaatgta atggttttaa attagaatat aagtctaaat taggatatat
      301 tctaaatatt aaagaccaag ataaattcga gcattatcta agaaacttac cctcagattt
      361 agttgaaaat ccagaacaac ggttggagtc tataatagta gagcttttag taaatgaagg
      421 ttataaaact atagaacagt taagtaaaaa atttctagta agttcatccc aaataaaaaa
      481 tgatttaaaa aagatagatg aaaagataaa ggatacagaa ttaaaactag agcgaaaggc
      541 tcattatgga attaaaatag aaggttctat aaaagaaatt caaagtatat tggtggatag
      601 ttattttaga gggaatagaa atataacaga gtacaggaat aagtttatag ataatttaaa
      661 attagctaat attagaagta caataaaaaa tgtacttaat gaacatgatt tagaagcaaa
      721 tttaacagaa cttgaggaaa tattagcaca aattattata ttgtatataa gagttagtat
      781 gagagtagtg ggaaatgcta gcgatttaaa attatataca gaagatttaa ttatagataa
      841 attattagat aaaattttta gagataaaaa atataacctt aattatgatg aaaagtatta
      901 tttaaaggag cttataaaac taaagactaa agataagaaa gccactataa agaatattga
      961 taagaataaa cttcaagata ttatgttcga attttttaga gagatagata aaaaatataa
     1021 ctctaatttt ttagaagata aagagttttt taacttattt tacttacata ttgcatgttt
     1081 aatagaacgt ataaagaaga accacaagat aataaatcct ttttcagtta aaataagtca
     1141 acagtatcca actgttttta atttatctat tcaattttca aaaatcatag aaaatgagta
     1201 tcatataaaa ataagtcaag atgaaatagg gtttatagct actcacattg ctgttccttt
     1261 tgaaaagaga gaagaagcta attttaataa aaaatataaa atagcaataa tatgctcttc
     1321 tggtggaggt agtgcatttt tgataaattt aagacttaaa gaaatttttc caaatgctga
     1381 gataagaaat ttttcattac tagaagaaaa agctgtaata gaatttgcac cagatttgat
     1441 tttttcaata acaaatcttt tatttgaaat aaatgctcca gttatactta taaatgaaat
     1501 tttagacgaa ttggattatt tagatattaa agaaagtgtt agatttgcag accatatagg
     1561 aaatatatca agcccaaaac aatatatatt aggtctattt gataagaatc actttaggtg
     1621 tataaaagat aaagctaagt ataaggatat ccttgatagt atgtcaaaat gtatagttga
     1681 cgagggagct tgttctccaa cctatcccaa agatgtatgg gaaagagaaa gttatttgag
     1741 tactatttat acaaatgggg tggctatacc tcatccaatc gagatgacag gaaacaaaaa
     1801 tataatatca gtagctttga tacaaactga tattatttat gaaaataggg tgcctaaaat
     1861 tattttcatg atttcattaa taaagggaaa tttagaactt cataagcaaa tatctaaata
     1921 tttaagtaag attatggcaa ataaggatat ggtagacatg ctaaataagt cacaatctta
     1981 tgaagagttt atgtataagt taaaaatcta tctgggaggt tagacatgaa tatagagttt
     2041 tttaagaggt tgtcagaagc agatggtata gcatctaatg aagaagaggt tagacatgtt
     2101 ttattggaag aactaaaagc ttatagtgat aaaattatct gtgatgggct agggagtatc
     2161 atcttttcta aaataaaaga tgagagtgca ccaaacgtaa tgatttgtgc tcatatggat
     2221 gaagttggat ttatggtgag aagtatagac aaacttggta tgattcactt aataacaata
     2281 ggaggagtaa aacctcttgc acagtttgta caaaaagtta gaattacaac aaaagaaggg
     2341 aagaaaatcc ccgctgtcat aaatgctaca tataataatg gtaaggctga aaatatatat
     2401 gcagatattg gagcatatac agaagaagat gtatataacc tagggattaa tgttggggat
     2461 atggtaacat atacaacaag ctttgaggaa tttactttac cagaccgatt agtgggaaag
     2521 gcttttgatg ataggatagg atgttttgtg atgggagaag ttttaaaaga acttaggaaa
     2581 gaaaatctaa attgtaatat tcactttgcg gctacaagta gtgaagaggt tggaataagg
     2641 ggagcaaaga catcaacaca gttaataaat ccagatatag tatttgtaat agatgtagca
     2701 tgtgctaaaa atgagtttgt gagagactat acaaataaga aacaaattga taaaggtgta
     2761 atgctaatgc atagagatag gactctagtc cctagtagaa aaatgataga ttatgtaatg
     2821 gaaattgctg ataaaaataa tataccactt caacatgata tgtttgaatc aggtgggact
     2881 gatggaggag aagcacatct cgtaaatgaa gggaaacctt gtgtagtaac ttgtgtacca
     2941 gttagatatg gtcattgtgc attttcaata gtaagtaata aagatttgga gaacattata
     3001 agattgtata cacagttaat tttaaatttt gatgaagaac aatataaaca tataagaaat
     3061 tttatctaaa ggagaataag aaaatgtcaa cagaagtaca aataatggaa cttatatcaa
     3121 ctgcaggaga gagtaaatcg aaagcttttg aggctttaaa aaaagtgaag tcaaaagatt
     3181 ttaaaggagc aaaattgtta atagaggagg ctcgtaaaat agatattgaa gctcacagga
     3241 tacaaacaga attaataact caagatttaa atgaagatgt tgaaagtcca gcaatgagtc
     3301 ttttaatggt acatgctcaa gaccactata tgacttctca gctagcaaaa gacttgatag
     3361 aggctttgat atattgtctt gataagtaat tgtaatatag aaatagatta tttatgtaaa
     3421 aattctttta aaagtttaga tgcttttaag agataataaa aatacaaact ttaggaggaa
     3481 ttattatgaa aatattatta tgttgtgcag gtggattttc tactaatatg ttaatgcaaa
     3541 atatgaaaaa ggtggtaaaa gagagtgcta aactcaatga aaaagatttt gagtttacag
     3601 ctataccagc agattcaata gaaagtgaag ttgatgattg ggatatagta cttataggtc
     3661 ctcaaatagc tcacaaaata gacttcttaa aaggaatatt agagccaaaa aataagcctt
     3721 ttgcagtaat agataaagat gtatatggaa aaatggatgg agctacagtt atgaagttgg
     3781 cattaataac ttataagaaa catcaattag ctcaaggata ataaaaataa tgagggaaaa
     3841 gggagattag atatgttttc aaaatttgaa gcatttatga atagagtatt tatgccttta
     3901 gctcataagg ttgacaatca acgtcacctt ggttctataa aggctggtat ggttgctatg
     3961 acaccattca caatattagg aagtatattt gcaatacttc cagcacttcc aaatatgctt
     4021 ggagaaaata atccaataag caaattcata cttagcaatt cagcaatatt ggatttacca
     4081 gtaagtcttt ccataggttt aataggatta tatgcttgta tgtgtattgc ttttaatctg
     4141 gggaactact ataaattata tataccaggt tgtgttgcac tatcaacttt ttctttttta
     4201 ttcctagtag cctcatttac accagatgga gctttagaaa taggaaattt aggagctaaa
     4261 gggctattta caggtatgat agcagggctt ggttctgtgg agttatataa tttttgtaat
     4321 aagaagaata tcacaataaa aatgccagaa aatgtacctg attttgtatc taggtctttt
     4381 gaattaatac ctactactat aatagtttgt ggaactttta tagttgctcg ttttatttca
     4441 ttaaaagtat ttggagaatt acctccacag atacttactc aatttttatc tccattagta
     4501 ggaagtatgg ataacccatg gtcagttctt gcacttaact ttgctatatg tactatattc
     4561 ttttttggaa tacattcatc agtatttagt cctataacta gacctataat gattacattt
     4621 atagctgaaa acatagcagc tatgcaggct ggagaaacaa tacctcattt ctttacagca
     4681 ggagcttcaa gtgcattctt tggatttaca ggatgtggaa ttagtatagg ttgtgtaatt
     4741 gcatgtatgt tatcaaaaaa tgcttcctat aagaagattg gacgtgtatc attattccct
     4801 gcattatttg ggataaatga accaatatta tttggagcac cgataatatt aaatccaata
     4861 atgtttatac cacatgtatt tggtggagct ataataggta cactaccaat gttttttatg
     4921 cactggggat ggttagcaaa gccagtattt gaccctccat atgtaggggt attcttagaa
     4981 ggatttttaa ctaatggaga ttggcattct atattagcaa atgcattgca attagtatta
     5041 gcagttttat tatattggcc attctttaaa ataatggaac gtcaacaaaa taataaaaaa
     5101 caaaaagaag aaactaagaa tatattctct aaagaagaag aagacttatt ggatgattta
     5161 ggattggatt tctaatattt tgttatgaat aatacaatgg agagattagt atccttaatc
     5221 tataaaagag gtgttgacgc tcttttgatt aaagcaaaaa atactaaaag atatatagga
     5281 gctttatcag gtagtggtgt atatgtgctt gttactaagg ataaaaatta tcaaattcta
     5341 gatggcagat atattgatga agcagataaa aaaacgactg gtttcataaa aaggattgta
     5401 tctcaaggtt cttatatacc aactataatt gagttattaa aagaattaaa tataaaaaag
     5461 ttagcaatag aaaaccaagc tatgagtata caagagtatc tttcactaaa aaatgaaggt
     5521 tttgaaatca atttaataac aaatgaatta tggaagctaa gggctataaa atctaaagaa
     5581 gaaattaaac ttattaaaaa ggcttgtgag attacagatg aagtatttga tgaggttatt
     5641 tctgaaatta aaaaagatat gactgagcta gaagtatcag ctctaattca gtatcatgca
     5701 ttaaaaaaag gtgcaagtgg aatgtctttt gaaacaatag tggtatcagg agaaagaggt
     5761 gcaatgccac atggaagacc tacaaacaaa aaattgaaag tgaatgaagc tataacaata
     5821 gattttggtg ttgtttatca agggtatcaa tcagacatga ctcgtacaat atctataggc
     5881 aaacctccta aaattattaa agagatatat gatgtagtat tggaagcaca attatctgca
     5941 atagagtcaa taaaggaagg aactagggca agtgatgttg acaaagtggc tagaaaaatt
     6001 atagataaac atggatttgg cgaatatttt aatcatggac tgggtcatgg tataggtctt
     6061 ggagatggtg aagttcccac tttaaatcca aatagtgaag atatattagt ggaaggaatg
     6121 gtaatgtctt gtgagccagg tatttatatt ccaaatgtag ggggagttag aatagaagat
     6181 gatattgtta taatagatgg aaaaggtgtt ccactcaata agacatccaa agagtttata
     6241 atattaggag agtaaaatta tgaaatataa ttttaataaa gtattaaatc gtaaagaagg
     6301 aaattgtaga aaatggagca accatgtgat taaagaaaaa tttggcttaa atgaagatgc
     6361 aattcctatg gatttagctg atatcgattt tgagtgtgct cctgctataa aagaatctat
     6421 cttaaaaaga gcatcagtag gagattatag ttatactttt ataggagatg acttctatga
     6481 tgctattata aattggaata aaagaagatt tgatgtatat atagaaaagg attggataaa
     6541 attaactttt ggtactgttt caacccttca ttatatagtc caagcatata ctaaagaagg
     6601 agagggtgta ttgataaata cacctgctta tgaccctttt gcagaggctg tagtaaataa
     6661 taatagaaag ttgtgttgta gctcattaaa attagataac aatagatatt atttagattt
     6721 agaggatatg gaaaatcaaa tgaaatacga gaatataaga ttgcttatat tctgtagtcc
     6781 acaaaatcca tcaggtcgta tatggacaaa agaagaacta tatcaagtgt cagagttatg
     6841 tttaaagtat aatgtaattt tggtaagtga tgaaattcat agagatataa tatttaaaga
     6901 ttatagattt gtatctttgt ggaatgctca tccagaaatt tataaaaata gtataatctg
     6961 tgtatctcct aataaaggct tcaacttagg tggattaaaa acatcatatg tattaataag
     7021 agatgtaaaa attagggaga ctttattaga aaggctaaaa agtaattcaa taacttctcc
     7081 aaatgtattt gcaataccag caatagttgc agcatataat gaaagcgaag aatggctaga
     7141 tgctatgact agttatgtag aggagaattt tgaaatagtt tatgatttct ttgaaactaa
     7201 tataccaaaa gctaaagtga tgaagtcaga ctcttctttt ttagcctgga tagatgtaag
     7261 agaagtattt agaaatgaag aagaatctaa agaatttttt agacatgcaa acttaactat
     7321 ggtagttgga agttattttg tgaaagatgg agatggattt attcgtatta atgtggggtg
     7381 tcctagagaa acattaaatg aggctctaaa tagaatcaaa aaaacgtata taagtatgta
     7441 ttgttaaatc ataaaaaaag tatctatggt ttatataaaa acacattatt tagttatcaa
     7501 atattactgg aattttgtat tccagtaata ttttttgatt aaggaatatt attgaggaat
     7561 attaaataat attatttcat acccttatat taacttatat aaaaatattt tattattttg
     7621 ggaacgggtt agctaacctt tatctagtgt atatttatat tgtaagaagc agtaaaaact
     7681 tttcctttac taagtttatt aataaacttc ttatctttat aaattttgtt agaatttata
     7741 ctatcagcaa gaccatacca aggttctata cataaaaaag gagcagtaga attagtttca
     7801 ctataataag gagtccaaat accaactaga gggaaatctt ccatagatat agttacatat
     7861 ttactaccat cattattaca tatagacact tcatctatgt tggtgtatat caaagcatca
     7921 ttcttgaata cttctggatt taactgtaag ttttttaatt ttccaatagg tattatatca
     7981 tctgtaaaag aaccatttaa attgattttt tctacatcat ttttactttt aaactcaagg
     8041 tagtgtttag aaaaatcatt ttgttcgtaa aaaggaaggt tgaatgcagg atgtccacct
     8101 atagaaaaga atatatcttt agaatcagta ttttttacaa tccattctac ctcaatacta
     8161 ttatcattta atgtatagtt tatgattagc tcaaaagaat atggatactt ctttaaagta
     8221 gattcattat ctgttaattt ataagttaca aatgtatctc ctttatctac aatctcaaag
     8281 tccatatctc tagcaaaccc atgttgagtc atgctatata aattttcttc tattattgtt
     8341 tcattatcaa aaagtttacc tactataggg aacaatatag gtgattgtct accccaatat
     8401 tttttattac catcccaaag aatgtttttg tcgtattttt tagaaaatat tttagttagt
     8461 tcagcaccaa aactgcttga ttcgataatt aaattttcat tttgtaatat attcatttaa
     8521 aatctccttt attatatttt atgagtatat tatactataa agtataaaga ttattaaatt
     8581 ttatttgata ttatatttga aaggtagatt ttagtatatt ttgaaaattt tgttttgaaa
     8641 atttgggatt atttaagaaa ttttgtatta taaatagaag gaaatagata aattgtatag
     8701 aataaaatat attaagataa gttaataatg ttaaatggga agtgagttta tggaaaaacc
     8761 aaatacaaaa gttgttaact ttcaagagta taaagacaga aaaaaccaaa gagaagtaaa
     8821 tagagacgaa cttttaaagc ttataaaaaa aattgtgact tcaaaatagg gagttgttaa
     8881 actccctatt ttaatgggtt tcataataat taagacattc taagtccatc tatataatct
     8941 ttgaattgtg agaaatctga ttcactactt aattctatgg tttctgaacc ttccgtataa
     9001 aaattcttaa gatagatagt ataattatta tcaacacaat catgataaat acatctatat
     9061 ccatcttcat acaaactttt taacttagaa aaatcatcca caatatcatc tccttaaagt
     9121 tcttttaaat atatttttac aaaaagcatc taattgtatg caaattgaca atgtaaaata
     9181 aaggaaatga acataaaatg tagaataatt tttatatgta tgtaaaaata aaaagaaaaa
     9241 gttgaaattt ttagaaaata agagtatttt ttatctaaaa agagagttta aaaaataaaa
     9301 aaatgttggg aggataaaaa atgagtttat tagaaagtat atcaaaaaac atatattccc
     9361 tagataactc ttctattgaa aaaacaaagc agagattgga taggcttata catccaactg
     9421 gaagcttagg aaagatagaa gatatttgca tgcaattagc aggaatattt ggcaatgaaa
     9481 attttgacac aagtaaaaag gttataattg cttttgcggg agaccatggt gtatatgaag
     9541 aaggcgtagc acctgaccct caaaatataa caaaattaca gtttccaaat ttttcgaaag
     9601 gtctatgcgg agttggagta ataagtaaat ttgtaggggc agatgttgta gctgtagatg
     9661 taggaataaa ctgtgatgaa aaactggatg gggtattaga ctacaagata agaaaaggga
     9721 catcaaatat ggcaaaaggt ccagctatga gtaaacaaga agcaattaga tgtttagaga
     9781 taggtattga aatagcagag cagtgtatag aaaaagatta taaggttatt ggtattgggg
     9841 agatgggtat agcaaatact actccaagta cagcaataat atctgtaatt tcagggtgtg
     9901 acccgttaga agttactggt ataggtgcag gtcttaagaa agaaagacta aagcataagg
     9961 ctgaagtaat tagaaaggct attgaaatta ataatccaaa tccaactgat ggagtagata
    10021 ttttatctaa agtaggaggg tttgaaatag gctcaatggc aggtgttata ttaggttgta
    10081 gtgctaatag gattccagta gtaattgatg ggtttatttc ttatgcagca gcattaattg
    10141 catataagat aaatccaaaa actagagaat atatgatagc atctcattta tcagcagaat
    10201 caggaactaa aagagcatta gacatattaa aattagaccc tctattaaat atggatatga
    10261 gattaggtga gggaagtgga gctgctttag catttgatat aatagaagct tcaaactata
    10321 cttataaaaa tatggcaact tttgatgaaa ttgacatggg aagataaata taaaaattta
    10381 atatgtagga gtgttttgat gagtaaaatt attctagtaa caggaggagc taggtcaggg
    10441 aagagtaact ttgcagaaaa actatgtttt gacagaaata ataatacagc ttatatagca
    10501 acctctattc catttgatga tgaaatgaaa gatagagtaa aaaaacatca agagaataga
    10561 cctagaaact ggaatactta tgaaatatat gaagatatat attccataat taaggagata
    10621 tataaagaac atgaaactgt tatactagat tgtgtaacat tattagtaaa taatctaatg
    10681 tttgaacaca atctaaatat agaagaatct agtcaagaag aaataaacaa attagaacaa
    10741 tacataaaag aacaagttgt caaacttatt gaagaaatag aaaaaacaaa tctatacttt
    10801 gtatttgtaa cgaatgagtt aggtatggga attgttcctg ggaataaact tagtagaatt
    10861 tatgctgata ttgtaggtcg tataaatcaa tatatagctt cgaaaagtaa tgaagtttat
    10921 tttgtagtta gtggcatagc tatgaagata aaggagtagt atgcaattat gaaaagattt
    10981 attttaatac tacagttttt gacaaggata cctataaaat taaatgtagg atttgatgat
    11041 gaattttata agtcaatagt atattttcca cttgtaggtt ttgtcatagg tatattatcc
    11101 tatttaatag gatggatttc tatgctttta tttgaaccat ttatagcttc tataataata
    11161 actctagctg gtgtcttaat tactggagga cttcatatag atggattagg agatacattt
    11221 gatgcaatat atagttatag ggataaagaa aagatgcttg aaataatgaa agattcaaga
    11281 ttaggtacaa attcacttct agcaattatg tttgtacttt tattaaaggt tggattcgtt
    11341 tacgatataa taagtaataa ttcattgtgg gttataattt ttatgccaat gattgcaagg
    11401 ttaggagtaa tgttattgac atataaaact gtgacaccaa gagaaaaagg aatgggaaat
    11461 ttatttatag gaaaattaac tacaagtatg ttaataactg ctataatata cacattattg
    11521 atagttgctc ttattactaa atttatattt ttattaccta atatagtgtt gattaaagta
    11581 ttaggttcga ttatagtggt ttttgtattt ataatattat tcaaaaaaca tatttataaa
    11641 aaaattgacg gagtaacagg agatatatta ggctgtggaa tagaactttc agagcttgta
    11701 tatttaattt acatatattt gttaattttt atgttttttt aaagtaaaaa gtggtgataa
    11761 tatgataaga ttaatattaa taagacatgc actgacaaat gataataaaa aaggaagatt
    11821 atcaggccat ataaatagtt gtattagtga agagggcaaa ctacaaataa ataaaattac
    11881 cagatatctg agtaatgaaa acatagataa ggtttataca acaccatcta cacgtactaa
    11941 agatacagta gaaaaaatat caaaattgaa attattagaa atagaagaaa aagaagcttt
    12001 aagagaaatt agttttggag actttgaagg aagaactttt gaagaaatta aaatcaaata
    12061 tccaaatgag tttgaaaaaa tgataaaaga aggtaacaac tatagatatc caaatggaga
    12121 aagtttgata gactcttata aaagagtggc aaaagaaatt gataatataa ttttagaaaa
    12181 taatagtaat ttagatacaa agacaatact tatatgttct catgcaggaa caataagaaa
    12241 tataataaca catttgattt ctggtagtta taagtatcac tggaacttta aaattgataa
    12301 tgcatctatt acagttttag aaattgatgg tggatttgct gtaatagata aaatgaattt
    12361 tacagacttc atataattta atcaaaacca aacattacaa tctgtgtttg taatatatct
    12421 gtaaaattag caatgaaaat agaatattag attaaaatta ggttcttaat tagatgttaa
    12481 aagggaaaaa ggttagatgc ctttgcagcc cccgctactg tgaaaccaac gaaactataa
    12541 ataccactgt cagtttgatg ggaaggttat agaagtagga tgaagttaag ccaggatacc
    12601 tgcctaattt aatttaaaac aagaggtctt cggggtgaaa gatttttaat taatatatat
    12661 aaaagtccta tctttagaag tattaggact ttttttaatt tgttttttag taaattttaa
    12721 caaggaggga tggaaagtgg ggaaaaaaat aatgctacaa ggaacagctt caaatgtagg
    12781 taaaagtgtc ttagtagcag gtttgtgtag aatatttaag caagatggtt atactgttgc
    12841 tccttttaag tcacaaaata tggctttaaa ttcttttata actaaagaag gattagaaat
    12901 gggaagagca caagtatttc aagcagaggc tgcgaaaata gagccaatag ctgatatgaa
    12961 tccagtttta ttaaaacctt ctggaaataa taaaagtcaa gttattgtaa gaggtaaagt
    13021 tgtaggagaa atgccttctt ctaagtatca tgattataaa ttggagttag tagatgtgtt
    13081 gaaggaaaca tttaatagct taaattcaag gtatgatatt gtagttatgg aaggggcagg
    13141 aagtaatgca gagataaact taaaagatag agatatctct aatatgggta tggcagaaat
    13201 agctgatgca cctgtaataa tagtcggaga tattgataga ggaggcgtat ttgcctcatt
    13261 agcaggaaca atgcttttat taaatgaaga tgagaaaaga agagtaaaag gtgtaataat
    13321 caataaattc agaggaaaaa aagagctttt acaagatggt gtaaagatgc tagaagacat
    13381 cataaaagta cctgttatag gagtagtacc ttatacagat ataaaaatag aagatgaaga
    13441 cagtgtaact acgagattta agaaaaaaat gaataaaagt gatatacaca tagaaataat
    13501 tagaacccca catatgtcaa attttacaga ttttaatata tttgagacac aagaagatgt
    13561 aagtataaga tatgtagact atggagaatc tttaggaaat ccagatatat taataatacc
    13621 tggaactaaa agtactatag acgatttaac atatataaga aaaagtggtc ttgaatcgca
    13681 aatcaaaaat ttacatagtc aaggtaaatt gatatttggg atatgtggtg gataccaaat
    13741 gttaggtaaa aaattaaaag acccttatca tgttgaaagt gaaattgaag aagttgatgg
    13801 aataggtctt ttagatacag aaacaacgtt tgagaaagaa aagactacaa cacaagtaga
    13861 agctactatt gttgaatgta tcagtgaata catgaataac cttaagggaa aaaaagtaaa
    13921 aggttatgaa attcatatgg gaattacaaa gcgaggtaat gatgtaagaa gtttagattt
    13981 aataactaaa aaacttgata aagaagtgaa ttatactgag ggaagtgtaa ataaagaggg
    14041 gaatgtaatt gggacttatc ttcatggaat atttgatgaa ataagcttta caagagcaat
    14101 tttaaacaat ataagaaaga aaaaaggttt aggagaatta gaaagcaaag taaactcttt
    14161 taatgaattt aaggataaag agtatgataa gttggctgat tttttaagag aacatttaga
    14221 tatagagaaa atatatgaga ttatgaaata atgatgacct ttatttataa tttaggtagg
    14281 aggtgtatta tgaaaaaaat tttaattgcg ggtactaata gtggagtagg taaaacaaca
    14341 atatcattag gaataatgca agctttggtt aagagaaata tgaaagtaca gccatataaa
    14401 gtagggccag actatataga cccttcatat cacactttta taactggaag acattctaga
    14461 aatttagatt cttatatgtt agatgatgaa aaaataaagt atatagtaaa taagtcctca
    14521 aaagatgcag atatttcagt aatagaaggt gttatgggct tatatgatgg ttttggaata
    14581 gatttagata attgtacaag ttcacatact tcaaaggtat taaagtcacc tgttatatta
    14641 gttataaatg gtaaatctat ggcagcatct agtgctgcta tggtacttgg atataaggaa
    14701 ctagataaag atgtaaatat aaagggtgtt atagtaaata atgtaaagac taatagccat
    14761 tatcaaataa ttaagagttc tatagaaaaa tattgtaatg ttgaagtatt ggggtatttt
    14821 cctccaaata acaaattctc tttggaatct cgacatctag gattagttcc tagtgtagaa
    14881 atgaaatcac taagggaaaa attttatacc ttagcagatg aaattgaaca ctatatagat
    14941 attgacagaa taattgaaat atcagaaagt gatgaatttg agagtgattt tgattttcaa
    15001 actttgatag aaaataataa aataaaagaa catgttaata ataaatcaat agctatagca
    15061 tatgataaag cattcaattt ttattataga gaaaatatag agcttttaga ggaacttggt
    15121 ttagaaataa actattttag ccctcttgaa gatgcatcag tgccaaatgc agattatata
    15181 tatattggag gaggttttcc agaaatattt ggaaaagaat tagaagcaaa tgagtcaatg
    15241 agaacttcca tattagaagc acataacaat aatataccta tttatgcaga atgtggagga
    15301 ctgatgtacc taggtgagaa actattgaat caagaagaac aagaatttaa tatggttgga
    15361 atatttagtg gaattagtaa gatgacatct tctttaaaga gatttgggta ctgtattggt
    15421 gtagccaagg aaaatactct tttatctaaa aaaggggaat ctattaaagg gcatgagttt
    15481 catcattcaa tatttgaaag tgatgaaaaa tgtgtatatt caatgagaaa agaaagagat
    15541 ggaaatatag tagaagagtg ggatggtggt tatagtaaag gaaacacttt ggctacatat
    15601 cttcatactc atttttataa taacttaaat tgtatagaaa attttttgaa aaaaggaaat
    15661 gaataaatga gtattttatc tatttatata ggatatgtga cagacttaat tgtaggagac
    15721 ccatattctt ttcctcatcc agtgagattt attggtaaac ttataaattt tactcaatgt
    15781 ataataagaa aggtctctaa aaatgatagg cagttaaaat ttgggggatt tatattatgg
    15841 cttataaccg ttggaataac atatttaaca acttatgcta tagtgaagtt atttagtttt
    15901 aatatattat tgagtattgt agtgaatagt tttataattt atacaacact agccacaaaa
    15961 tgtttaaaag acgaagcatt aaaaatttat aatgtattaa aaactgaaga tatagaaaaa
    16021 tctaggattc agctatctta tatagtaggt agggatacta ctaatttaag tgaacctgaa
    16081 ataataagag caactgtaga aactgttgca gaaaatactg tagatggtat aattgcgcct
    16141 atgttttatg catttattgg aggagcacca cttgctatgg cctataaagc tatcaataca
    16201 ttggactcaa cagttggata taaaaataaa aagtataaag atattggttt tgcatctgct
    16261 aagattgatg atatagccaa ttatatacca gctagaatat ctgtcattct tatgacaatt
    16321 ggaagtttct ttttaaatta taactatagg aactgtttta aaatctctat tagagataga
    16381 aagaatcata aaagtccaaa ttgtgctttt tcagaaggtg cagtatctgg tgctctagga
    16441 atacaactag gaggtaccaa cgtttatttt ggggaaaaag tatataagcc aacaataggg
    16501 gataagctta gggagataga taaagaagat ataattaaaa ctaataaaat tatgtatgca
    16561 tcttcatttg tatctatgtt ggtatttaca ctaatatatg tgttatgtag gatgatttta
    16621 attaacttaa ttatataaat tattgtttgt taacttaagg gggacataga tgaaggattt
    16681 agggcatggt gccaatgtag atgaaatggc aagattatat gggaaaaacc caaaagaaat
    16741 aattgatttt agttcaaaca taaatccaaa tgtattacca aatttagaga gatacatttt
    16801 aaaaggatta gaagagtgta ggaattatcc agatataaat tatacaaact tgagagaaaa
    16861 tatatcaaaa tatatagata taaatccaga ctttataata cctggcaatg gtgcaacaga
    16921 agtgatttat ttgcttatga agagtattaa aaaaaaattg gctataataa atccgacttt
    16981 ttcagagtat agaagaagtg ctgagttaaa taatttagat attatagatt tagaattaga
    17041 tttagaaaat aactttaaac ttaatataga tataataaaa gaaaatataa aaagatttga
    17101 tagtttattt atatgtaatc ctaataatcc gagtggtaat gtccaagatt taaaggaact
    17161 tgttcattta ctagataaac ataataaggt actaataatt gatgaaactt tcatggaatt
    17221 tgttgaagat gaaagtaaat atagtttggt taaatatata gaatcaaata aaaatatatt
    17281 tataattaaa gctgtgacta agttttttgg tatgcctggt ttaaggctag gatatggtct
    17341 tacaagcaac actgaaatta tgaacaaaat ttatgaacac aaagagccat ggactatcaa
    17401 ttcttttgca gatatactat ccaattttat ttttgaagat aaagagtata ttaaaaatag
    17461 taaagaatat tacatagaag aaagaaaata tatgttacaa gagttgagaa acataagaaa
    17521 tataaaggtc tatgatacag atgctaattt tatcttgata agaatatata aaaaaacaac
    17581 taaagaattg aaaaaagatt tgtttaagca aggaaacata ttagttagag atgcgtctaa
    17641 ttttatagga ttagatgata gttttataag agtggctata aaatctcatg aagataacaa
    17701 aattcttata gaaaatatga aaaatttatt gggtgattaa aatgaaatct tatggaatat
    17761 gcccagcatc ttgtggagaa tttgtacaag gtataattga tgatgaagaa tatttatgtt
    17821 catatgcaat agatatgtat tctaaagtgt atattgaaga aaaattagta gatataaatt
    17881 tagggagata taagtcaagg ttagcaattg aaaaggtatt tgaaaaattt aatttaccta
    17941 aaaaatatac taaaaatatt tctttaaata taaatagtaa aatacctgtt ggaaaaggaa
    18001 tggcaagttc aactgctgat ataggagcta ctataaaggc aacattatca ttgatagata
    18061 aagatttaag tagcgaggag atttctaaat tagcagcaga aatagaacca acagattcta
    18121 tatttataga taaaaatagt atatttaatc ctctaaatgg aactgttata aaatacctag
    18181 gaaatctgac caatgcgaaa gttgtaatac ttgaaccaaa caaagtacta gatactatga
    18241 agattagatt gaggcaagat tataataaat taaaagtaga gaataaagaa gtaataaaaa
    18301 aatcatttgc tcttttagaa gaaggattga aaaaaaataa tttatctcta gttggagagg
    18361 cttgtacttt gagtagctta gcaaatgaga atatagaaaa aaaagagtgt ttaaatgaaa
    18421 taataaaaat atcaaaaaaa tatggagcat atggagtaaa tattgctcac agtggaactg
    18481 tagtaggtat tttaattgat aagtctatga atgataaaaa gatgatagat gccttatgtg
    18541 aatctaacat aaattctgtc tataataaaa tttatacaca aaacataatt aatggaggaa
    18601 ttaaggggga aatagaatgg aatacataaa gaatcctatg aaaatagaag aaaaaagttt
    18661 tgaattgata caagaaataa tagacgaaat tagaccagat tataaattta agaataatat
    18721 cgaagaaaaa ataataaaaa gagctataca tactactgct gattttgatt acttagatat
    18781 attaaaaata tcagaagaag cagttgatag tataatagat gcattaaaaa ataatgcaag
    18841 tatttatact gatacaaata tggctcttag tggtataaat aaaagaaagt tagaatcatt
    18901 aggatgtaag tataaatgtt tagtggcaga tgatgaaact gtaaaattag ctaaagaaaa
    18961 aggaattact cgttctatgg cagcagtaga aattgcagct aaagaagaag gaagaaaaat
    19021 attcgtttta ggtaatgctc caacagcttt atataaagtt atggagatga aatcagaagg
    19081 tagattagat ttagatgcag ttataggagt tcctgtaggt tttgtaggag cacaagaatc
    19141 aaaagatgaa gttgaaaaaa cagatatacc atatataatt tctaaaggta gaaaaggtgg
    19201 cagtaattta gcagcagcaa tagtaaatgc tatattatat actatgtagg tacctatatg
    19261 gaagaatatg tatatataga tggaaaaaaa tatagaagag gatatactac aggttcatgt
    19321 gcaacaggtg catctaaggc tgctgtatat atgttgatta caaaaaatag aatcaatact
    19381 ataaatatag atactcctaa ggggatacca ttactactaa aagttgataa tataaatatt
    19441 tctgatacat ttgtggaatg ttccataaaa aaagatggtg gagatgatat agatgcaact
    19501 cataccatgg atatttatgc aagagctgaa attgtagcta aaaatgataa gaacaaaggg
    19561 tatcttactc taaaggatat agatagttta agcaccaata gtgagtgtaa aagtgagctc
    19621 tataagttta ttagggttta tggcggaact ggtattggtg tggttacgaa aaaagggctt
    19681 agtgtggatg ttggtaagcc tgctataaat ccgactccac ttaaaatgat aaatcatgaa
    19741 ataagaaagc ttataggaga taattttgaa tctatacttg gaaatgataa agttttaaaa
    19801 ataacaatct ttgctccaca aggagaaact gtagcaaaaa aaacatttaa tccaagatta
    19861 ggtatagttg gaggaatatc tataataggt acaactggga ttgttgaacc tatgagtgat
    19921 gagggttgga agaagtctct atctatagaa cttcagatga aaaaagaaca aggcttagat
    19981 aaaataatat tggttccagg aaatcatgga gaacagttta taagagaaaa attaaactta
    20041 gatattaagt atgtagttag ggtaagtaat tttataggct atatgattaa agaagctcaa
    20101 agaataggat ataagaaaat cttaatggca ggtcatatag gtaaatttat aaaagtatct
    20161 gctggtattt ttaatactca tagtaaggta gcagatgcta gaagtgagat attagtggca
    20221 aacttagctt taatgggagc tagatacgaa tttttaaata aaataaatca atgcgtaact
    20281 actgaagaag ctgtagagct aataaataat agtgaatata gagaagtata taatatatta
    20341 agtaataaat gtagggagag agtaaagcaa tacttgaatg aagattcaga tgatattgat
    20401 gtggaagtaa tcatattttc aatggataag tctttattag gtaaatcgga taatacagat
    20461 gatttagtgg aggtctttat atgataaaca taataggtct aggacctggc aacttagact
    20521 atataacaaa aaaaggtgaa aatttaattt ccacttctga tgtattaatt ggtggtaaaa
    20581 gaaatttaga gtctataaaa aattttgaag gagaaaaaat agttctggat tctaatttaa
    20641 gagaaattat agagtacata aataacaata aagaaaagca aatatcaata atagcatctg
    20701 gggacccatt aatatatggg ataggtagat atctatctaa aaatattgat aataagatgt
    20761 taaacatggt ttctgggatt agttcattac agtacatatt ttcaaaaata tatgtagata
    20821 tgaatgatgt atatattaca agtagtcatg gtaaagtacc agattttgat tacatattat
    20881 cacataaaaa agtatgtatg gttacagatt caaagatagg accaaaacaa ataagtagag
    20941 aaataataga tagaaatcta aataaaatta ttgttgtagg agaaaatctg tcatatgaca
    21001 atgaaaaaat aacaatagca aaaccagaag agataataag aatagacaat tttgatatga
    21061 atgttgtggt gattttagat gaggaatagt gaatttataa caggtaaagt tccaattact
    21121 aaggaagagg ttagagcaat atcaataagt aagttaaatt tagtaaatgc taaaaacctc
    21181 atagacatag gggctggtac aggaagtgta agtgttgaag ctgcatataa ttatcctaat
    21241 ttaaaggtta tatctataga gaaaaatgat gatgctatag agcttataga gaaaaatata
    21301 aaaaaattca acttaaaaaa tatagaagtg ataaaaggat gtgctccaat taatctaggg
    21361 ttaaatgtta aagttgagtc tatttttctt gggggaacag gaaataatct ggaagaaata
    21421 atagaatggt ctaaaaaaaa tctcgttaca ggaggaagac ttgtagctaa ttttataata
    21481 atagatacat ttaatcaaac tttaaaactg cttaaaaagc atggatttaa agaaatagat
    21541 gtatgtgttt tgaatgtttc taaactggag aaactaggaa aaggtgaata ttttaaacca
    21601 ctaaatccta tatatataat atcttgtgaa aaaggggaaa atgatgatga ataaagtaca
    21661 ttttgtagga gcaggacctg gagataaaga gctgataaca ttaaaaggat acaaattatt
    21721 aagtaatgca gatgtagtga tatatgcagg ttcacttgtt aatcctgaac ttttggaata
    21781 ctgtaaagaa gattgccaaa tacataatag tgcacatatg gatttacaag agataataga
    21841 tgttatgaga gaaggtatag aaaataataa atctgttgta agactgcaaa caggtgattt
    21901 ttctatatat ggttcaatta gagaacaagt ggaagattta aataaattga atatagatta
    21961 tgactgtact cctggagtaa gttctttttt aggggcagca tcatctttag gggttgaata
    22021 tacagttcca gaaatttctc aaagtgtaat tataacaaga atggaaggca gaactccggt
    22081 tccagagaaa gaatcaatac aatcatatgc taagcatcag acatctatgg taatattttt
    22141 atctgttcaa gaaattgaga aagttgtgtc taaattactg gaaggtggat atccaaaaga
    22201 tactcctata gcagttatat acaaagcaac ttgggcagat gaaaaaatag ttaaaggaac
    22261 tttaagtgat atagctgtaa aagttaaaga aaacaacata aataagactg ctttaataat
    22321 ggttgggaga tttttaggag aagagtataa taattcaaag ttatatgata aggatttcaa
    22381 acatgaatat agagggtaat attgctataa tctgtattac agaaaatgga aaaaaattag
    22441 ctctaaagat aaattctcta gtaaataact gtgttatata tcaagtaaaa aataaaaaaa
    22501 gtaatttagt tgttgaagaa acatgtgtac atacagttca aaagaagtta agtgattttg
    22561 taggagaaat atttagtaaa tttgattaca tagtatttat aatggctaca ggtatagttg
    22621 tacgaagtat agcaccatac ataataagta agtttagtga ccctgcaatt ttggtaatgg
    22681 atgaaaaagg caaaaacgta ataagtttat tgagtggtca tattggaggg gcaaacagct
    22741 tgacacagta cataagtagt cttattgatg ctaatccagt aataacaaca gctactgata
    22801 taaatcaaaa agcttcctta gatatgatag caaaaaaact aaatgcacat attgataatt
    22861 ttagagaaag tgtaaaagaa gtaaattcta tgctagtgaa taataaatgt gttggattat
    22921 atgttgatga aggatatcaa gttgatacta ggggatttaa acttatagaa agtaaggatg
    22981 agttaagaga tagtttttca aacacatata tagaaaaatt agttgtagta agcaataaaa
    23041 taaattgtat tgaagatatt gagaatttaa acctagataa taaatttgaa cattgctata
    23101 gtacccaaaa aataataaag gtaattccaa gagatatagt gataggaatt ggctgtagaa
    23161 gaaacacaga tagtgaactg atgtataatt cacttgaaga gtttcttaat aaacaaaata
    23221 tagatattag agcaataaac acagttggaa gtatagaact gaaaaaagac gaaaaagcaa
    23281 taattgaatt atcaaaaata ttgaaggtgc catttaaaac attttcagta gatgaaatat
    23341 ctaaggttga taatcttttt gataaatctg attttgttaa aaaaaatgtg ggtgtgtatt
    23401 cagtagcaga acctgtagca tatcttttga gcaaaggtaa tttgttagtt gaaaaacata
    23461 aatataaagg aataactttt tcagtgggga gaataaaaat atgatatacg ttgtaggaat
    23521 tggaccagga agtaaagata caatgacttt agaagcaata aaagcaatag aagattctga
    23581 ggctattgtt ggatataaga cttatataaa actaatagaa gaatttatac aaggtaaaga
    23641 aataatccaa aatggtatga ggcaagaagt tgatagatgt aaacaagcag tggaggaagc
    23701 aaaaaaagga aaaaaagtag cagttgtaag cagtggtgat gctggaatat atgggatggc
    23761 aggtcttatt ttagaattaa tatctaaaga agatgaagat ataaaagtaa aggtaattcc
    23821 aggcgttact gcaagtattg gtgcagcagc tatacttgga gctcctatta tgcatgattt
    23881 ctgtcatata agtttgagtg atttaatgac tccgtgggaa gtaattgaaa aaagattgag
    23941 attggctgct gaagccgatt ttgtaatttg tttgtataat ccaagaagta agggaagaag
    24001 tgaacatcta agcaaagcat tcaaaataat gggtgagttt aaaggtggag atacacctgt
    24061 tggagtggta aaagatgtag gtagagaaaa agaagaaaag tttgtatgta cttttgaaaa
    24121 tatggacttt gaaaaagtcg atatgactac aatggttata ataggaaata agtctaccta
    24181 tattaatggt gaattgatga taactccaag aggttataca gtatgatttt aattttagga
    24241 ggaacttcag atagtttatc tatatgtgat aagataaatg aactgaaaaa tcaaccatat
    24301 atcctttcag ttactacaga ttatggaagg gaacttgcac aacaacatgc agagaatgta
    24361 attttaggta agcttggaaa agaagatatg ctaaaattta taaaagataa taatatagta
    24421 aaaataatag atgccactca tccttatgca gttgaagtat ccaaaactgc tattacttgt
    24481 gcaaaactat tgaatataga ttatataagg tttgagagaa aatctttaat tgaagaaata
    24541 gattatgata ataaatttat agtagataca attgaagaag cctgtgaaat agcaaataaa
    24601 atagggacaa atatattcat aggaacagga agtaaaaatt tgaacagatt tatagaggaa
    24661 ataagtgata aaaatttagt tgttagagtt cttccaacga gtgaagtcat attaagttgt
    24721 gaaaaattag gtttaagtgc agataatata gtagctatga aaggtccatt tagccagtat
    24781 ataaatgaag aaacatataa acattacaat atagatttag tcataacaaa agaaagtgga
    24841 atagctggtg ggttcttaga gaaggtaaat gcatgtaaaa ctttcaatat accagtaata
    24901 attataaaaa gagaatcgat taactacgaa aatgtattta atgacataaa taaaattaaa
    24961 gacatacttg tgtaattttt aggaggagaa tatatgaaga aagccttatt agtagtgagt
    25021 tttggtacaa gctatcatga aacgagagaa aagacaatag atatatgtga agataaaata
    25081 aaaaattcgt taaaaagcca tgattttttt agagcttata catcaaatat gataataaat
    25141 aaaataaaaa aaagggatgg aatagaaata gataatccaa tacaggcttt agataaaata
    25201 tatgaacaag gatatgatga agtaataatc caaacacttc atattatatg tggcgaagaa
    25261 tttaacaaat taaaagaaca agtagatgga tattcttcta aattcaaaaa gattgtatta
    25321 ggaagacctt tgcttacaca tattgaagat tatcaggaag ctgtagaggc aattaagcat
    25381 caaattcctc atatggaaag taatgaagca gtagtattta tgggacatgg aacacttcat
    25441 gaatcacatt cagcgtatcc agcattggaa tacatgctta gggataatgg aataaatgct
    25501 tatgttggta cagttgaagg gtatccagaa atagaacatg taataagaag attaaaagaa
    25561 ggaaatataa aaactgtaaa tttgatgcca tttatgttag ttgcaggaga ccatgctata
    25621 aatgatatgg caggagatga agaagattct tggaaaacaa tattagaaga aaatggattt
    25681 aacgtaagag ttcatttaaa aggactagga gaaaattctt atatacaaga taaatttgtt
    25741 cgtcatgctg taaaatgtgc agagaatgcg aagttctatg gtataggagc agggccagga
    25801 gatggttctt tacttacgat aaaagcggtt aatactttaa agaaactgga tattctatac
    25861 actccagaat caaaaaaggg tggagatagt ctagctttat caatagttag tgaatattta
    25921 cctgaatcag tggaaataaa gtctaggcat ttcccaatga gttttgatgg gaatgaaaaa
    25981 agtttagctt gggacaatgt ggcacaagaa attatatcag atgttaatga tggtaaaaat
    26041 gtaggatttg ttacattagg agaccctatg atttatagta catatgtata tataatgaaa
    26101 agaatattag gagaggtaga agtcgaaact ataccaggaa tttcatcatt ttccaatata
    26161 gcatctaatc agaattttcc attagtcatg gataaagaag ctttagtggt aattccttgt
    26221 actatggaag atgataagat agagtatgct ttgcaaaatt ataattcaat agtattgatg
    26281 aaagtatata aaaattttaa agaaataata aaaaaattag aaaagtatga tttaatagaa
    26341 catgctattt tagtaagtaa ttcttcccaa gaaagcgaaa atgtgtttac agatttaaaa
    26401 gaagcgcacc ttgaagataa gatatcttat ttttctacta tactagtaaa taaagataat
    26461 aaaataaaat aggtgaaagc aatatttaaa ggagacttaa tatgaatata gtggttggaa
    26521 ctaggggtag taatctagcg cttatacaaa cagaatgggt aataaatgaa ttaaaaaaga
    26581 agtatccaga aattagtttt gagattaaaa tcataaagac aaaaggagat ttaattcaaa
    26641 atgtttcact ggataaaata ggtgataaag gattgtttgt caaagaaata gagcaacaat
    26701 tattagatgg aaaaatagac atagcagtac atagtatgaa agatatgcct tcttatttag
    26761 cagatggact aaaatttgct catacgccta agagagaaga cccaagagat gttctaatac
    26821 ttagagaagg atataaaaat ttagatgatt tgcctcatgg agcagtaata ggaacaggaa
    26881 gtaaaagaag gaagtttcaa ttattgaaac aaagacctga tttaaatata gtacaagtta
    26941 gaggtaatgt ggaaactagg atacgaaaaa taaaagatga aaacatgcat ggtattgtgt
    27001 tggcagcatc aggaataatt agagctaatt tacaagataa aataagttct tatttacctg
    27061 ttgatgttgt tattccagca cctgcccaag gagctttagc tatagaaatt agaagcaatg
    27121 atagtacgat agagggaata gtaaattcac tgaaggatga gaatacagaa atccaaatat
    27181 tggctgaaag aggtttttta gatggtgtaa atggaagttg tcatattcca atagcagctt
    27241 attgtgaaat aatccaagat aaaatacatc ttactggctt atatggagat tcagaaggaa
    27301 aaaaagtagt aatcaagtct atagatggag atatttcttc tcctagagag ttaggtttaa
    27361 agttagctaa acttgtattg aaggagtatg agaattatga agggtaaggt ttacttagta
    27421 ggggcaggac caggtgatta taaactcatg actttaaaag gtttagagtg tataagaaaa
    27481 tcggatgtaa tagtatatga tagattagca aatagtagtt atttaagaga agctaagcct
    27541 gattgtgaac ttatatatgt aggcaaggca tcaagtaatc atattttgcc acaagaagat
    27601 ataaatagaa ttatagcaga aaaagctaaa gaaggaaaaa ttgtaactcg tcttaaagga
    27661 ggagaccctt atgtatttgg cagaggggga gaagaagcag agactttagt agatgaaggt
    27721 atagaatttg aagtagttcc aggtataact tctgctatag gaggtttatg ttatgcgggt
    27781 attcctataa cacatagaga ccatgcttcg tcatttcatg taataacagg tcatttgaag
    27841 ggtgatgata gtggagaatt aaactggaat gctcttgcaa ataacaaagg tactcttgtg
    27901 tttttgatgg gaatttcaaa cctagagaaa ataagtgaaa acttaatgaa agaaggaaaa
    27961 gataaggata cacctgttgc tcttataagt tgggcaacta gatataatca aagagttgtt
    28021 acatctacac ttgaaaatgt ttatgaaact gctattaaag aagaagtaaa accgccaact
    28081 cttatagtag ttgggagtgt tgtaggactt agagaaaagt tgaacttctt tgaaagtaag
    28141 cctttatttg gaaaaagtat tgctgtaact aggtctagaa atcaaaatag tgtattggta
    28201 gaaaaaataa tggatttagg tggaaatcct atagaaatac ctactattaa ggtagaaaaa
    28261 atacaaaata atattaactt agaaaatgaa ataaaaaata taaataagta taattatcta
    28321 atactaacta gtaaaaatgc cgtagaaatc ttctttgaaa agatatatga aatgaatttt
    28381 gatttaagga tattatctaa tttaaaggta tgtgctatag gttctgctac atcaaatgag
    28441 ttgaaaaaaa gaggaattat agcggatata gtaccaaaaa aatttgtagc agagtcactg
    28501 tatgaagagt tagcgcctat tttaaatgat gacgataata tacttatacc tagagcagaa
    28561 aatgctagag attatctagt tgacaaactt aaggctattt gtgaagtaac agaagtacat
    28621 atatatagaa cagtaataga tgaggagaaa aaaggtgaga tattagatat tttaaattct
    28681 aatgacatag attatataac atttactagt tcctcaacag taaataatct tgttgaaatt
    28741 ataggaaaag aaaatattaa aatattagaa aaaataaaaa cactatctat aggtcctata
    28801 actacagaga caataaatag tttgggaata aaattgtaca aagaatctgc tacatctact
    28861 atagattcgt tagtagattt aattataaaa aattaatttt ggaggcttga atatgttaag
    28921 aagaccaaga agactaagag caagtaaagc aatcagaaat ttggtaagag aaactaaggt
    28981 gaatgtagaa gacttaatat atccactgtt tgtagtagag ggggaaaata taaaagaaga
    29041 aattaaatct ttaccagatg tctatcattt atcaatagat atgctagaag acgaaataaa
    29101 agaaataagt gagttgggaa ttgaacatat aatgttattt ggtgttccag atgaaaaaga
    29161 tgagtgtgct tcacaagctt ttaatgataa tggaatagtt caaaaggcaa ttagaaaggt
    29221 aaaagaaata aattcagata taaatgtaat aacagatgtc tgtatgtgcc aatatacaag
    29281 tcatggacat tgtgggattt taactgatac tggttatgtt gataatgata aatcattgga
    29341 gtatttggct aaaatatctg taagccatgc taaagcagga gctgatatgg ttgctccatc
    29401 tgatatgatg gatggaagaa tacaagcaat aagagaagct ttagatgaaa atggattaga
    29461 aaatgttgga atcatggctt atagtgttaa gtatgcttca aattattatg gaccttttag
    29521 agaggcagct cattcagcac catcttttgg agatagaaaa acatatcaaa tggacccagc
    29581 aaatagcaat gaagctataa tagagactga acttgatata ttggaaggtg cagatatact
    29641 tatggttaaa cctgcattat catatttaga tattataaga agagttaaag ataattttga
    29701 cttacctcta gcagcttata atgtaagtgg agaatactct atgcttaagt ttgctgtgaa
    29761 ggaaggttta ttaaatgaag atgtaattct tgaatcaatt acatctataa aaagagcagg
    29821 agcagatata ataataactt attttgctaa agacattgct aaactgttga aaaaataaat
    29881 tctagtagta taaagaatat caaaaagtat aggaattgta attaactatt atacagtagt
    29941 tttgtttaga catttattga agaataatag aaactaattt gcaatttcta tactttattt
    30001 atgtaactaa ataaaatttg gaggtagaag atgctatatc ctattaattt aaaattagat
    30061 gagttagatg tagttataat tggtggtgga gaagttgcat ataggaaatg taaaaatttc
    30121 ttagagttta acaaaaatgt tactatagtt tctaaacaaa ttttaaataa attttatgat
    30181 ttaaaaggaa atataaaaat aataaaagat gattataaag aaatatatat aaaagatgct
    30241 tctgtaataa ttgctgccac aaataataga gagttaaata tggaaatagg attatattgt
    30301 agagcaaaaa ataagctagt gaatgtagtt gataatatag aaatatcgaa ctttactgtt
    30361 ccatcatata taaaaagagg agatttactt ataagtgtgt ctactggggg caagagtcct
    30421 tcattatcat caaagataaa aaaagaaatt gaagagagat atactgaaga ttatgaggag
    30481 tatttaaatg tattaggaga tataagaaaa aaggttataa aaaaatacga agataaaagt
    30541 aaaagaaaag atgtgcttaa tatgttggta acacttgacc tagaagaact taagaagttt
    30601 gatatataaa aaataaaata aaaaatgtta aaaaggtgtt gacggat
//
LOCUS       DAEOSP010000027        29986 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.027, whole genome shotgun sequence.
ACCESSION   DAEOSP010000027 DAEOSP010000000
VERSION     DAEOSP010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29986
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.027"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            159..299
                     /locus_tag="KRH54_000983"
     CDS             159..299
                     /locus_tag="KRH54_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578652.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(474..794)
                     /locus_tag="KRH54_000984"
     CDS             complement(474..794)
                     /locus_tag="KRH54_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBG8578653.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(914..1651)
                     /locus_tag="KRH54_000985"
     CDS             complement(914..1651)
                     /locus_tag="KRH54_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBG8578654.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1866..3689
                     /gene="acd"
                     /locus_tag="KRH54_000986"
     CDS             1866..3689
                     /gene="acd"
                     /locus_tag="KRH54_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBG8578655.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3904..8202
                     /locus_tag="KRH54_000987"
     CDS             3904..8202
                     /locus_tag="KRH54_000987"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBG8578656.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8406..8867
                     /locus_tag="KRH54_000988"
     CDS             8406..8867
                     /locus_tag="KRH54_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBG8578657.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8897..10051
                     /gene="nusA"
                     /locus_tag="KRH54_000989"
     CDS             8897..10051
                     /gene="nusA"
                     /locus_tag="KRH54_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBG8578658.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            10058..10339
                     /locus_tag="KRH54_000990"
     CDS             10058..10339
                     /locus_tag="KRH54_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBG8578659.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10329..10640
                     /locus_tag="KRH54_000991"
     CDS             10329..10640
                     /locus_tag="KRH54_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBG8578660.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10662..12602
                     /gene="infB"
                     /locus_tag="KRH54_000992"
     CDS             10662..12602
                     /gene="infB"
                     /locus_tag="KRH54_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBG8578661.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12645..13025
                     /gene="rbfA"
                     /locus_tag="KRH54_000993"
     CDS             12645..13025
                     /gene="rbfA"
                     /locus_tag="KRH54_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBG8578662.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            13006..13989
                     /locus_tag="KRH54_000994"
     CDS             13006..13989
                     /locus_tag="KRH54_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBG8578663.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNIGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEDVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQIKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSGYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            14002..14907
                     /gene="truB"
                     /locus_tag="KRH54_000995"
     CDS             14002..14907
                     /gene="truB"
                     /locus_tag="KRH54_000995"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBG8578664.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            15038..15967
                     /locus_tag="KRH54_000996"
     CDS             15038..15967
                     /locus_tag="KRH54_000996"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBG8578665.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            16071..16328
                     /gene="rpsO"
                     /locus_tag="KRH54_000997"
     CDS             16071..16328
                     /gene="rpsO"
                     /locus_tag="KRH54_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBG8578666.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            16568..17413
                     /locus_tag="KRH54_000998"
     CDS             16568..17413
                     /locus_tag="KRH54_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG8578667.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            17538..19649
                     /gene="pnp"
                     /locus_tag="KRH54_000999"
     CDS             17538..19649
                     /gene="pnp"
                     /locus_tag="KRH54_000999"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBG8578668.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            19915..20658
                     /locus_tag="KRH54_001000"
     CDS             19915..20658
                     /locus_tag="KRH54_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG8578669.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            20816..22063
                     /locus_tag="KRH54_001001"
     CDS             20816..22063
                     /locus_tag="KRH54_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG8578670.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            22098..22343
                     /locus_tag="KRH54_001002"
     CDS             22098..22343
                     /locus_tag="KRH54_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBG8578671.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            22353..23576
                     /gene="dapG"
                     /locus_tag="KRH54_001003"
     CDS             22353..23576
                     /gene="dapG"
                     /locus_tag="KRH54_001003"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG8578672.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            23689..24423
                     /locus_tag="KRH54_001004"
     CDS             23689..24423
                     /locus_tag="KRH54_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBG8578673.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            24546..26957
                     /locus_tag="KRH54_001005"
     CDS             24546..26957
                     /locus_tag="KRH54_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBG8578674.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            26960..28018
                     /gene="mnmH"
                     /locus_tag="KRH54_001006"
     CDS             26960..28018
                     /gene="mnmH"
                     /locus_tag="KRH54_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBG8578675.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNDVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            28012..29346
                     /gene="rimO"
                     /locus_tag="KRH54_001007"
     CDS             28012..29346
                     /gene="rimO"
                     /locus_tag="KRH54_001007"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBG8578676.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHKIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            29333..29875
                     /gene="pgsA"
                     /locus_tag="KRH54_001008"
     CDS             29333..29875
                     /gene="pgsA"
                     /locus_tag="KRH54_001008"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBG8578677.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNIPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
BASE COUNT        12098 a         3190 c         5695 g         9003 t
ORIGIN      
        1 ttaataggta aaatatccta ttgttttgta gatatatcct aattatgtca aaaaataaaa
       61 aaatttttat ttattagagt ttttggaaat aataataaca gatattataa ttaattaaca
      121 gtttttgtaa gtatttgaca atagagaggg tgtaacaaat gggaataata gagtcagcat
      181 caaaattggc tgagatggtt catttattgg ctgttgaaaa ggggataact gatatagaag
      241 catgggatga agctgtgaaa gaatacagca aaatttatga agaaagaaga aatgaataga
      301 atgataaaaa atgaaaactt gatgataaaa aataacttta tttaaagaaa ttcaattttt
      361 atagaagtat taaaaataat ataaataaaa agatggtatt tcaaatgtaa gtaaaaaatc
      421 attttgggat accatcctta ttatttattt taataatata taagtaataa atcttactta
      481 tccatatttt ttatgtaacc tcttttttct agctttttta tatgctcctt acgtatttca
      541 tcaagattaa taccatattg ttcttctagc ttaaacatca tagtaacaca tgtctgcgct
      601 acatccatta gttccttagc agttttcttt ataatatcaa cttcatctaa atcattattt
      661 tctccactta gatttctgaa tttacctata cattcagcta attcaccttg ttcttcaccc
      721 attttgataa atgtagattc tagtgttggg tctaaattat taagctctgg taaagatata
      781 gtttttagtt tcataaaagc tcctttcaag ttattcgtat ttatactaaa acatcataac
      841 aaaaataata ctaaaaaaaa actttaaatt caacataaaa acttacatat ataataaaaa
      901 tcgtgaaata aatctatatt tgtcttaata cagcctcata taaaacttct ccaataggtt
      961 tatttattac aagagaagct gacttatcat agactgtatt tgctttatta ataagagcaa
     1021 tataatcacc tttgtaataa tctataaaac ttgctgctgg gtacacagct aatgatgtac
     1081 ctcctataat caataaatca gcattactta tagcagaaat tgttttagta atgacatcac
     1141 tgtcaagagc ttcctcataa agtactacat ctggttttac tatagaacca cagttgtcac
     1201 agtaaggaat attacctccc agatttaaca tagattctaa gtcaaaaaac ttaccacatt
     1261 tagtacaata atttctatgt acagaaccat gaagttctag cacattttta cttccagcca
     1321 tttgatgaag tccatcaata ttttgagtta tgacagcttt aagcttaccc atttcttcga
     1381 gttttgctaa agctatatga gcattattgg gttttgcatt aggatatatt aatttatcct
     1441 tataaaattc aaaaaattct tctggatatc taacaaaaaa agtatgtgaa actaattgtt
     1501 cagcagtaaa ttgtttattg agtttttggc taaataatcc agtagaactt ctaaagtctg
     1561 gaatattaga ttcagttgaa acacctgctc caccaaaaaa tacaatatta ttgtgattag
     1621 ctattaagtc ttttaagcta tttgcatcca tagttacacc tccaaattat tatatataaa
     1681 ttgtactaca tattaggtcg tttatgtttt attaatttaa ataaatatat agtttaataa
     1741 gttaaataaa gggaatataa ataaaatgta tacagagtat aaatagacaa tatttactaa
     1801 aataacaagt tgtcgtataa ttatattaaa attgttttag tgtataagaa gggcggggga
     1861 agtacgtgaa gaaaaaagca gctttagcaa cattggctat gttaccctta ggtgtggtaa
     1921 atgcacatgc tgatggagat ataggtatag tgactataaa ttatttaaat gtaagaaatg
     1981 aaccaactgc cgaaagtagc atagcctttg ttgctaaaaa agatgataaa gttttgatta
     2041 aagactcttc taatggatgg tataaaataa aagctgaatc tggacaagaa ggttgggctt
     2101 catcaaaata tatagcaaaa tcaaatagtg actctttaag aacgtctaca aataaagaaa
     2161 aacaagttat ttcaaatagt ttaaatatga gaaatggagc aggtacaagt tacagagtaa
     2221 taactgtact aaaaaaaggt caaaaagtag aagtaatatc agagagtaat ggatggtcta
     2281 aaatcaaata tgatggaaga ctaggatatg tatctagctc ttatttagga gacgtttcaa
     2341 actcaactaa taaatcaaaa actaaacaag tgaatacaac ttcactaaat gtaaggagtg
     2401 gaccaaatac aagttatggt ttgttaggca agttgccaaa aggaagtaaa gtagaagtaa
     2461 tatcagaaag taatggatgg tcaaaaataa agtataatgg aaaagatgcc tatgtatcta
     2521 gtatgtactt atcggatgta agtcaaagta attcagacaa ttctagtcaa agcaatgata
     2581 aaaagaatac tgataaggtt gtaaatacag cttctttaaa tgtaagaagt ggaccaggtt
     2641 ctacatatag taagttggga aaagtttata aaggaagtaa agtaactgta ctatcagaaa
     2701 gtagtggatg ggctaagatt aattttaaca ataaagaagc atttgtagta ggcaattatt
     2761 tatctacttc agcagatact tcaaataata actcaaatag taactctgat aacagttcta
     2821 atagtaatgg taataattct tcatcatcag gtcaagtaaa tggtatgtca ggtataagtg
     2881 gggctaaaat tgattataaa tctttgagtt atactttaga atctcatata agtaaacaag
     2941 ttgagaaagc agcatcagga ggaaatgtga tagctccaag taataggaaa agtactccaa
     3001 gtcctgaatt tagtactttt tcagctcaaa gaacaagctc atttgtaaat gcaagttcaa
     3061 gtgatataga atattattta aatcctaaga attttacaaa tactactaaa ggtatgatgc
     3121 agtttttaaa gattaacagt tatagagatg gtatttcaga atcaagtctt aactcgtatc
     3181 ttaatggttt atcttctagt gtatttaaaa atcagggagc agcttttata aatgctgcca
     3241 agaagtataa tatagatgtt gtatatctag tatctcatgc tatgtgggaa actgcttatg
     3301 gtaagtctac acttgcccaa ggtcaaacat taacatctta taagggacaa cctcttagta
     3361 agccagttaa agtatacaat ttttttggta taggagcaat agataaaagc gctaatgttt
     3421 caggtgcaga agcagcgtat tccaatggct ggactagtgt agaagctaca atagatggct
     3481 ctgcaaagtg gatatcacaa aattacgtaa atagttctaa gtacaatcag aatactatct
     3541 acaagatgaa gtggaattat gattatacat ggcatcagta tgcaactgat gtaaactggg
     3601 ctaatggaat ttctggaatt atggaaaatt taattggtct ttatggagga ggaagtagtt
     3661 tggtttttga agttcctcaa tataagtaat acataaataa aaaatataga ctatgggtag
     3721 ttttcatttt atttaaatta tctatagtct ttttgtatat aatttataat ctgtaaattc
     3781 aatgttaaca aaacttttat gttataatat taattctaag taaatataag tgagtaatag
     3841 gtagaaatag ataaaaagtg gtaataatat atataaagat aattttgaga aaggaggata
     3901 tatatggaaa gtattaagga atatttggat aagttagaaa ttaataatag tggtcttgga
     3961 aagcaactta aagaagttta tataaataga gtagtttatt ttaaggaaga taagatagtt
     4021 tatttttatc taacttctaa agatattgtt tctcatgaac ttttagataa atttaaagaa
     4081 gaattgatgt ataaactcga ttattttaaa gatatgagga tgaagataag atttactggt
     4141 cttgaaagaa aatccaataa agatgttata aaaaaatatt ggaataatat attgtatata
     4201 ttaaagtatc tttgtccatc tattgcaggg tggtataaac aagtagaatt tctttgttta
     4261 gaagaagaat taaaaataaa acttccaaaa ggtatatttt atgagcgatt aatgaaaaaa
     4321 aatgtaactt atgtacttaa aaccgtgctt agcgaagagt tgggattaga tttaaatata
     4381 acaatagaaa aagctgttga tgaaaaggta aataaagaaa gactcataag aatcaatgat
     4441 agagaaatgg aagaaaaaat tagggcttta gaaattggaa aagttaataa ctgtgaagaa
     4501 aatgaagagg aaagctatgt cattaagtct gatgtagatg aaaatttaat ctatggagat
     4561 aatgcaaatg ctatgattga aaatatagtt gaattaaatg catcttctgg aacagttgca
     4621 gttgttggag atatatttga tgttgacact aaagaactta aaaacggaaa aatacttatg
     4681 atagcttcaa ttacagatta tactagctca ataagctgta aacttttttt aacagataca
     4741 aataaggatg gagtacttga aaatgttaaa aaaggagcat acttaaaaat caagggtgac
     4801 atagtttatg atacatatca aagagaaatt agtatgatga ttagtgggat aagaaaagaa
     4861 actagaatag agagagaaga taaatcagag gaaaaaagag tagaactcca tgcacataca
     4921 caaatgtctt ctatggatgc tatttgttct gttaaaaagc ttgtagagcg tgcagccaaa
     4981 tggggtcatt ctgctattgc tataacagac catggtgttg tacaaggatt tccagatgct
     5041 atgaatgcag gtaagtcaaa taatataaaa atactttatg gagtagaagg gtacttggtt
     5101 gaagatgatt ctctcattat agaggatgca aatgataaag aactttcaca gacatttgtt
     5161 gtatttgaca tagagacaac tggtttttct aatacaaatg ataaaataac tgagattggt
     5221 gctgtaaaaa tagaaaactt taagattgta gatagattta gtgagttaat aaatcctgaa
     5281 aaagatattt cttataagat acaggaattg acaggaataa ctaatgaatt aattaaagac
     5341 aagccaacca ttgaagaagt tcttcctaag tttatggaat ttgttggaga tagtgtatta
     5401 gtagcacata atgcagagtt tgatactgga tttatatcgc aaaaatgtag ggaacaaggc
     5461 ttgttatata ataacaaaaa agtagataca cttatgttag caagagtaat gttgccaaac
     5521 ttaaaaagac atagattaaa tgtcgttgca aaagaacttg gtattcctct tttaaatcac
     5581 catagagcag tggatgatgc tgaagctaca gcacttatat ttaataagtt tttgcagatg
     5641 cttacagaaa aaggtgcaaa aactctaagt gatgtaaaca atatacttgg aaagattgat
     5701 tacacaaagc ttagcacaaa tcatataacc ttgatagcaa aaaattctat aggtataaaa
     5761 aatttatata aaataatttc agatgcacat gtaaatcatt tttttagagc accaagaatc
     5821 cttagaagtg tattaaatga atataaagaa ggattaataa ttggttcggc ttgtgaagct
     5881 ggggtggttt ttcaagctgt aaagaaaaac gtgtctgatg aggaaatgaa aaaaataata
     5941 gatttatacg attatattga agtaatgcct atagataata atagatttat gattgataaa
     6001 ggagaagtaa aggacgaaga agagttaagg gagttgaata ggaaattaat tgatacagcc
     6061 aaaaaatttg acaaaattcc agtagctact ggggatgttc attttttaga caaacatgaa
     6121 gctgtcttaa gaaaagttct taaatattct caaggtttta aagtagatga agaagaaact
     6181 tatttacatt ttagaacaac agatgaaatg ttggaagagt ttagttatct tggagaagaa
     6241 ttggcatatg aagttgtagt tgaaaatagt aatctgattg ccgatatggt tgaagatata
     6301 aaacctatac cagatgatac ttatcctcca attatagagg gctcagatgt ggagttaaga
     6361 gaaatgtgtt acaaaaaagc caaacgaatc tatggagacc caataccaga aatagttcaa
     6421 aaaagactgg atagagaatt aaattcaata ataagcaatg ggtatgcagt tatgtatatt
     6481 atagctcaaa aactggttgc aaaatctctt tcagatggat atttagttgg ttcaagaggc
     6541 tcagttggct catcttttgc agctactatg agtgatataa cagaagttaa tcctcttcca
     6601 gcacattata tttgtgaaaa cgaagactgt aaatattcat tcttttatga aataggtgaa
     6661 tggggctcag gtgttgattt accagacaag gattgtccaa agtgtggaag aaaacttgct
     6721 aaaaacggtc atgatattcc atttgaggtt ttcttaggtt ttgaaggtga taaagaacca
     6781 gatatagact taaatttctc aggaaattat cagccaacaa ttcataaata cacagaagaa
     6841 ctatttggag aggggtatgt atatagggct gggactatag gtactgtagc agaaaaaaca
     6901 gcttttggat acgcaagaaa atatgtggaa gaaaataata taagtgtacc aaatgcagaa
     6961 gtacttaggc tttcaaatgg atgtactgga gtaaaaagaa cctcaggaca acatcctggt
     7021 ggagttatgg ttataccaga ctataaagat gtatacgatt ttacacctat acagtatcca
     7081 gcaaatgata cttcttgtgg tgttataact actcactttg attatcattc aataagtggt
     7141 agaatactta aacttgatat acttggacac gacggtccta ccattataag gatgcttgaa
     7201 gatatgactg gaataaatat aacagaaatt cctttagatg ataaagagac tatgtctcta
     7261 tttacatcta cagaagcatt aggtgttaca ccagaagaaa taaactgtcc tataggttgc
     7321 cttgctgtac cagagtttgg aactaagttt gtacgacaaa tgttacttga cacaaaacct
     7381 actacatttg cagaacttgt acgtatatct ggtctctctc atgggacaga tgtttgggta
     7441 aataatgcac aagacttagt tagagaagat atagttggat taaaagatgt tatatctaca
     7501 cgagatgaca taatgaacta tttaatattt aaaggattac ctcccaaaat gagctttaca
     7561 ataatggaga gtgtaagaaa aggtaaaggt ttaaaaccag aacacattga agaaatgaaa
     7621 aaaaataatg ttccagaatg gtatataggc tcatgtaaaa agattaagta catgttccca
     7681 aaagcacatg ctgttgctta tgtaatgaca tcatttagaa ttgcatattg taaagttcac
     7741 tatccagaag cattttatgc gacttatttt acgacaaaag ttgaggattt tgatgcagat
     7801 ttaattgtta aaggattaga tgctataaaa tcaaaaataa atgaaattga gtcactggga
     7861 aatgatgcaa ccacaaaaga aaaaggtatg ttgaccgttt tagaagttgc tcttgaaatg
     7921 tatgctagag gaataaaatt attaccagtt gatatatata aatcagatgc aactgagttt
     7981 atagttgtag gagaaaaaac attacttcca ccaatggcag caatacaagg tcttggagga
     8041 aatgcggcta taaacatcca aaatgaaaga aaaaatggtg aatttatatc aaaagaagat
     8101 ttgagaaaga gaacaaaaat atcaaaaacg gtaatagaaa ctcttactaa tcatggttca
     8161 cttgagaata tgagtgagag aaatcaattg tcattgtttt aagttagccc aaaaagttgc
     8221 aaataaaaga attttatgat atcatagtat agaaaatagt atactttgca gaaactaaga
     8281 gtgagacagc tgttctcact cttttatttt aaatatatta ttaaaaagct ggtttatttt
     8341 tattaaatat attttattta agattatata aactggtcat ttacaattga ataggaggaa
     8401 ctttaatgaa aaaaaattta gaagctacaa ttgaggagat tgtaactaaa ataacggatg
     8461 aacatggatt tgaaatggtt gatgttgaat atgtaaaaga agcaggagaa tactacttaa
     8521 gagtatacat agataaagaa gaaggaatat ctttaaatga atgtgaatta gttagtagag
     8581 aattaagtcc aatactagat gaaaaagacc ctataaaaga aaattacttt ttagaggtat
     8641 cttctccagg gcttgataga gcattaaaaa aagacagaga ttttgttaga tatcaaggta
     8701 gagatgtaga tttaaaatta tataaaccat taaatggatg taagcaattt gaaggtgaac
     8761 ttgtaggatt aacagaagat aataatataa aaatcattgc aaatggtaag gaaatggaat
     8821 ttaatagaaa agatgttgct attgtcagat tagcaataaa gttttagtgg tatattaaat
     8881 agggaggata taaaaaatga atcatgaatt tatggaagca ttagatgaat tagtagttga
     8941 cagaggtata gataaagaaa tacttataga tactatagaa caagctcttt taactgcata
     9001 caagaaaaac tttggttcag cacaaaatgt tagagttgaa tttgatagag acaagggaga
     9061 tataaaagta ttttctcaaa gagttgttgt tgatgaatca gatttatatg atactttctt
     9121 agaaatagag ttagcagatg ctagagagat aaaccctaat tatgaattag gtgatattat
     9181 agaacatgaa gttacaccta aaaactttgg aagaattgca gctcaaacag caaagcaagt
     9241 agttgttcaa agaataaggg aagctgagag agaaatagtg tataatgaat ttatagaaaa
     9301 agaaaatgaa atagttacag gagaggttgc tagagcaaat aaaaatgttg tttatgtaaa
     9361 tcttggaaga atagaaggta taatgactca aagtgaacaa ataccatttg agaattataa
     9421 agcaggtcaa aaaataaaat tctatatact tgaagttaaa aagactaaca aaggacctca
     9481 aatagttgta tcaagaagtc atccgggtct agtaaagaga ttgtttgaag aagaagttcc
     9541 tgaaatatat gaaggaatag ttcaaataaa atctgtagca agagaagcag ggtcaagaac
     9601 taaaatggca gtaagatcta tagatgaaaa aatagaccca attggagctt gtgttggacc
     9661 aaaaggactt agagtaaaaa atatagtgga tgaacttggc gatgagaaaa tagatataat
     9721 aaaatatagt gaagacccag cagagtttat atcagcagca cttagcccat caaaggttgt
     9781 tagagtagat atagatgaag atgaaaagtc tgccttagta atcgttccag attatcagtt
     9841 atcgcttgct ataggtaaag aaggtcaaaa tgcaagacta gcagcaaaat tgactaactg
     9901 gaaaatagat attaagagcg aatctcaagc agaacaagaa atgttaaatc ttgaaaattc
     9961 caaagaaata gaagctgagg atgttgaagc tgaatttatc gaccaagaag agttagatac
    10021 ttcagaggat aattttagtg aagaagagta aggagggatg attttgaaaa aagtaaaaaa
    10081 agtgcctcaa agaaaatgca tagcatgtca agatagagac tctaaaaaag agcttataag
    10141 aatagttaaa aataaagaag gaaaaatatt tttagaccca acaggaagag ccaatggaag
    10201 aggagcatat gtatgtaaaa gctctgaatg tctaaaaaaa gctataaaga gtaaagcatt
    10261 aaataaagct tttaaaatag atgttcctga tgaagtctat gataatctac ttatggagtt
    10321 agaagaatat gaaaaataat aaagaaaaag tactttcgtt cttagggctc gcaactcgtg
    10381 cagggaaaat agtgtctggt gatgattcaa ctttgcttga ccttaaaaaa ggaaaagtaa
    10441 atttgatttt aatcgcagaa gatgcatcaa ataatactaa aaaacttttt aaagataaat
    10501 caacttttag aaatattcca tatctatttt tttcaacaaa agaggaaata ggatttgcaa
    10561 taggaaaatc tccaagagca gttgtaggaa taaaagatga aaatttttct aaaaagataa
    10621 ttgaactaat agaaatttaa aataaaatag ggggtgatct tgtgtcaaaa acaagagtat
    10681 accaaatagc agaagaatta aatatatcaa atgaagagct tataaataag ttagctgaat
    10741 tagatataaa tgtaactgac aaagattcag tattagaagg tgaagagtta gaattagcac
    10801 ttgaaatgtt aggtgaagat ttatctcaag aaaatggaaa tgttatagaa atagatggaa
    10861 agttgacagt acaagtatta gcaacgaaat tagataaatc tccatcagaa ataattatga
    10921 agcttatgaa aatgggaact atggcaacaa taaatcaaga gataagtttt gagattgctg
    10981 ctttggctgc caaagactat ggatttgaat taacagtagc tgaaagtgat gatacagaag
    11041 ctttagaaat agaagcttta atggaaattg aagaagataa agaagaggat ttaaaaccta
    11101 gaccaccagt tgttacagtt atgggacatg ttgaccatgg taagacatct ttactagatg
    11161 caataagaaa aactgatgtg atttcaggtg aagctggagg aataactcag catataggtg
    11221 cttcagaagt taaaatcaat ggacacaaaa tagtattttt agatactcca ggacatgagg
    11281 ctttcacatc aatgagagca agaggagcac aagttacaga tatagcaata ttagtagttg
    11341 cagcagatga tggtataatg cctcaaacag ttgaagctat aaaccatgct aaagcagcag
    11401 gtgtaccttt aatagttgct attaataaaa tagataaacc aggtgcaaac ccagataagg
    11461 ttaaacagga attagcagac caaggattat tagttgaaga ctggggtgga gaagtaatag
    11521 cagttccagt atcagctaag aaaaaagaag gaatagatac acttttagaa atggtattat
    11581 tggttgctga aatggaagag ttaagagcta atccaaataa aagagcagta ggaactgtta
    11641 tagaagctga gcttgataaa ggaagagggc cagttgcaac agtacttgtt caaggtggta
    11701 ctttaacagt aggtgaccca atcgttgcag gtgttgcatg tggtaaagtt agagcaatga
    11761 taaatgcaaa aggtaagaga gtaaaaacag caggaccatc tacagcagtc gagatattag
    11821 gtctatcaga agttccacaa ggaggagacc aatttgtcga agttccaact gataagattg
    11881 ctagaagtgt agcagcaaga agacaacaaa tagttagaga tgaaatgtta aaatcaactc
    11941 aaagattatc actagatgct ctatttagtc aaatgagtga aggttctata aaagacctta
    12001 atatagttat aaaagcagat gtacaaggtt cagtacaggc agttaaacaa tctttagaaa
    12061 aattatcaaa tgaagaagtt caagttaaag taatacatgg tggtgttgga gctgtaacag
    12121 aatcagacat attacttgca gcagcttcta acgcaataat aataggattc aatgtaagac
    12181 cagttccagg tgcagaatct ctaggagaaa aagaaaatgt tgatataaga acatatacta
    12241 taatatacaa ggcaatagaa gatatacaag cggctatgac tggtatgtta gaccctgaat
    12301 atgttgatga agaaacagga aaagctgaaa taagagaaat atataaaata tctggtgttg
    12361 gaacagtagc tggatgttat gtgactaatg gtaagatatt tagaaattgt aaagtaagat
    12421 tagttagaga tagcataata atacatgaag gtgagcttgc agcacttaag agatttaaag
    12481 atgatgtaaa agaagttaat tctggatacg aatgtggtat gagttttgta aattataacg
    12541 acataaaaga aggcgatata gtagaagctt atataactaa agaagtagaa agaaaattat
    12601 aaattcgtat caaagaaaat cagttagtaa agaaggtcga tggtatggca tcatataata
    12661 gaacaagaag gatagcagaa gaaataagaa aagtcgtaag cactatgctt ataaatggag
    12721 taaaagaccc tagaataaca tctatggtga gtgtaactga tgtagaagtt acaaatgacc
    12781 taagatatgc ttatgtatat gtaagcattc taggtggaga tgaagaatcc acactaacag
    12841 gtcttaaaag tgctggagga tatataagaa gagaagtagg aaaaaatata aaacttagat
    12901 atattcctga aatagtattt aaacttgatg attcaataga aaaaggaatg tatatggata
    12961 gccttataaa aagagttaat gaaaaaaatg ctcaacaaaa tgaggatgaa aattatgatg
    13021 aataatatgg atagtattat aaactatatt tatgagggtg actattttat agttacctct
    13081 catatcagtc cagatggaga caatattggt tctacacttt caatgtatta tacattgaag
    13141 aatttaggaa aaaatgtata ttatgtactt gatgatgaag ctccattaaa tttaaggttt
    13201 ttggttgaag atgtaaaaat ctataagtct aatgaattta atatgaagaa ctattctcta
    13261 attgcacttg attgtgggga taagatgaga atatgtgtga gtgatgaaat caaaaataat
    13321 gcatcaaaat taatctgtat tgaccatcat gctagtaatg attcatatgg agatttaaat
    13381 tatgtagata ttgatgcatc ttctacatgt gaattagttt ataatttatt agttagattt
    13441 cagcaaataa aagatattaa tattataaat gaagatatag caacatgttt atatacaggt
    13501 cttgtaactg atactggaaa cttttcgtat tcaaatgtac atgcaagtag ctttgaaatg
    13561 gcaaaaaatt tacttgtttt aggtgcacaa aaaaatacta taatacaaaa tatatatcaa
    13621 agcaattctt ctggttatta caaattatta ggagaagctc taaagggttt ggagatattt
    13681 gattcaaagg tatctagtat agttttaact caagacatga tgaatagaaa taatattagc
    13741 tttaatgatg ttgatggaat aacaacatat acaagagaca taaaaggtat agaagttgga
    13801 attttattta aagagaaaaa acaaaatgaa attaaagtga gttttaggtc aaaaaattat
    13861 gttgatgtaa gtgaaatagc caagttattt ggtggtggtg gtcatgtaag ggctgctgga
    13921 tgtactatta gagatagtat tgataatgca aagaaaatgg tactagaggc agtgttaaaa
    13981 tcaatttaag gtgacagtaa aatgaataag attataagta tattgaaacc cacaggtatg
    14041 acatctcatg atgttgtaag tagagtgaga aagattttaa atataaaaaa agttggtcat
    14101 acaggtactc ttgacccaga tgcttcaggt gttttaccaa tctgtatagg gaaagctaca
    14161 aaagtatgtg aagttatttt aaacaaagat aaatcgtata tatgtgagct tactttaggt
    14221 ataagtacag atacttatga tgcttcaggt gaaattttaa aaaaagttga tgattttaaa
    14281 tttagtaatg aagacataga aagagctttt gacacacaaa gaggagagat aaatcaactt
    14341 cctccaattt attcagcttt aaaggtaaat ggaaagagaa tgtgtgattt agtaagaagt
    14401 ggtagacaat ctgaaattac tttaaaaaca agaagagtaa atataaaaga cataaaaata
    14461 ctaagtataa aaggtaataa agtaatgttt tatgttgagt gttctaaagg gacttatgtg
    14521 agaagtattt gccatgatat aggagaatat ctaggttgtg gagctcatat gtcgtttttg
    14581 aatagaacat cttctggtaa atttgactta gataattcaa taacattaga agaattagaa
    14641 ttattttatg aaaataaaac tttagacaaa tacttatatg atatagatta tgtccttgat
    14701 tcgtttaatt atgttgtatt aaaccctaat gctataaaat attatagtaa tggtggctca
    14761 atagatgata aaaggttttt aaagaataat ttcgataaag atgatgaatt tgtaagagta
    14821 tacagtacag ataatttctt aggattagga aagttatcaa aacacaataa tacaataagt
    14881 gttaaaagtg ataagatgtt catataacca atgtatttaa aaaaacaaca aaagagatag
    14941 tactataata acttctatag tctatctctt ttacttatgc tataatagtt tgtgaataag
    15001 ttaagagtaa attatatttt agcggggaac atttattatg gtgattataa aatctataaa
    15061 agaaatagca aacataaaag aaagtgtcat taccatagga aattttgatg gtgtacacaa
    15121 aggacatcaa gttcttatag gaaaaacagt tgaacatgca aaaaaagaaa atatacagag
    15181 tattgtattt acatttgcaa atcatcctgt aaattacttt agaccaaact caacaaaaaa
    15241 tattataagc aatgatgata aaataaaatt attagaagat ttgggaatag atatagttgt
    15301 aatcatacca tttgatgagt atatgactaa aatacctgca aaggatttcg tagatgagat
    15361 actagtaaaa aaattaaaag ctaaaaagat agtaattgga catgacttta catttgcaag
    15421 ggcaaaagaa ggcaatgtta atctattaaa aagcttagaa catgaatttg gttttgaggt
    15481 agaagtaata aaacccatca aaatcaatga tgtaagagtg agtagtactt atattagaag
    15541 tttagtatcc cagggtgata tggctaatgt caaggaatat ttgggtagaa attacaagtt
    15601 agaaggttgt gttatacatt ctaaacacct tggaagaact ataggattcc caacggcaaa
    15661 tattgacctt aagaataata tgctcgttcc aaagagaggt atttatgctt ctattgtaca
    15721 tattggagat gagacttatt ttggtgctac aaatgtaggc tataacccaa cagttaatgg
    15781 aaaaactttg tctattgaaa caaatatttt agagtttgat agagatattt atggggaaaa
    15841 tataattgtt gaatttttgg aaagaataag agatgaaaga aagtttaatt caatagatga
    15901 cttaaaaaat caattgtata atgatacaaa ttatgtgtat gaaaactatg tttgcaaaaa
    15961 caagtaatgt atgataaaat gattaaggaa aataaccatt actcggcatt tgaatactcc
    16021 aatcaatgct tagtaatagg cgtttctaaa aaaatacgga ggtaatctaa atgataaaca
    16081 aatcagaaat aataaaagaa tatggaagag ctgaagggga tactggttct ccagaagtac
    16141 aaatagcttt attaacagct agaataaacg agttaaatgg acatttaaaa actcataaga
    16201 aagaccacca ttcaagaaga ggactattaa aaatggttgg tagaagaaga aacttattag
    16261 cttacttaaa ggaaaaagat cttgaaggat atagagcatt aatagctaaa ttaggattaa
    16321 gaaagtaatt tcattcttta taaaaaagca ggtatagtgt atacctgctt ttttatagtt
    16381 aattcattat aaagtaaaat agttaataat aaaagtagtt tatatgttat aaaaaattat
    16441 aaaatgaata atattgggta agaatattaa aatagtatac aaatacataa tcaatttaaa
    16501 aggaaagaca ggataaatat agaaatttta taacaggata attagttgga ataaggagga
    16561 tattattttg aaaataaagt tagtatgcga tagtttgtgc gatattccag atgaaatatc
    16621 agaaaaagat tatatagaga tagttccact tacagttatt tttaatgata gggaatatat
    16681 agaaggagta gatataaaga aagaagagtt ttataaaaaa gtaaaagaaa tcaagcaaat
    16741 acctaaaact tcacaagcta catatatgga gtttaaagaa gtgtttgata agttcataac
    16801 agaaggttat catataatct gtatgacagg ggctgcaaac gcatcaggaa cttttcaaag
    16861 tgctatgata gctaaaaatg atgtaaatga aaatgaaaaa atacatatat ttgatactag
    16921 aaatttatct ctaggtagtg gacaatatgt tataaaggca tgtgaacttc ttgaagaagg
    16981 actgggattt gaagaaatta tagatgaatt agaaaatact cgcagtagtg taagattgct
    17041 ttttgcacca tatacacttg attttttaaa gcagagtgga agagttccag ttgcaactgc
    17101 tttgatagga aatatgttaa atataaagcc tatatttttc tttgataatg gggaagctaa
    17161 gttggtaaat aaggttagag gaataaagaa tatagcaagt aaattggtcg atataatttt
    17221 agaaatgaat gaagggtctt tagaaggaaa aatcgtcaca ataggttgtg gagataattt
    17281 acacgattgt gaaatattaa aagatgaagt aaataaaaga ataaaagcaa gaagagtatt
    17341 atttactcga ggaggagttt ctatttgctc tcatacaggg ccagatatat tggctataag
    17401 ttgttctaat tagtagtaat ataaggtttt tgcatatata tgtgaagata attttatatt
    17461 gttaatttat agtatttttg ataatatagt aaatagagtg atatacgtgt tttttaataa
    17521 gaggaggaca agcattaatg tttgaacata aaatatttaa aatggatttt gcaggaagag
    17581 aactttctgt agaaatagga aagatttgtg aaatggctag tggtagttgt atagttcgat
    17641 acagtgatag tatggtaatg gtaaatacaa ctaaatcagc caaacctaga gacggaatag
    17701 acttttttcc tttaagtgtg gattatgaag aaaaattata ctctgtaggt aagatacctg
    17761 gaggtttttt aaagagagaa ggcaagcctt cagagaaagc tatacttaca tcaagattga
    17821 tagatagacc aataagacct ttattcccaa aaggatttag aaatgatgtt caggtagttg
    17881 caacagttct atcagtagac caagattgta caccagatat agtagcaatg ataggttctt
    17941 caatagccct ttctatatca gatataccat ttaatggacc aacaggttca gtatgtgttg
    18001 gccttgtaga tggtgcattt gttgtaaatc caaacgcaga gcaaagagaa aaaagttcaa
    18061 tgcatctagt tgtttcaggt acaaaggaag caataatgat ggttgaagcc ggagctgatg
    18121 aagttccaga cgaggtaatg cttgatgcta ttttatttgc tcaccaagaa ataaaaaaaa
    18181 tagttgagtt tatagaaggt atagttgcag aagttggaaa agaaaaaatg cctgttgaat
    18241 tataccatgc aggagaagaa ataactcaat tggttcgcga atttgcaact gataagatga
    18301 aaaaagcagt tcagactttt gaaaagttag agagaatgga aaatatggat agagttaaag
    18361 aagaaacttt agctcacttt gaagaaactt tagaagattt tgaagatttt gttggagata
    18421 tagaagaagt attacaagat ataataaaag aagaagtaag aaaacttata gtacatgaaa
    18481 atgtaagacc agataataga aaacttgaag aaataagacc tatatggtgt gaaactggca
    18541 tgatacctag agctcatggt tcagccatat ttactagagg acaaactcaa gtacttaatg
    18601 ttgctacttt aggtgcttta ggagatgttc aaaaactaga tgggttagat gaagaagaaa
    18661 acaagagata tatgcatcat tataatttcc ctgcatatag tgttggagaa gctagaccat
    18721 caagaggacc aggaagaaga gaaataggac atggtgcttt agcagaaaga gctttattac
    18781 cagtaatacc atctcaagaa gaatttcctt atgctatcag attagtttct gaagtattaa
    18841 gttcaaatgg ttctacatct caagcaagtg tatgtggctc aacattatct ttacttgatg
    18901 caggtgtacc tataaaagat atggttgcag gtatagcaat gggacttata aagcatgatg
    18961 gtaaggttgc agtgctttca gatatacaag gtatggaaga ccatttaggc gatatggact
    19021 ttaaagtagc tggaacagag tatggtataa cagcaataca aatggatata aaaattgatg
    19081 gtatagataa agaaattcta caacgtgctt taaaacaagc taaagagggt agaatccata
    19141 tactaggaga gatgagaaaa actatatctc agccaaaacc agaactttca ccatatgcac
    19201 ctaagattgt taaaatgcaa ataaatcctg acaagataaa agatgtaata ggacctggtg
    19261 gaaagataat aactaagata atagacgaaa ctggagttaa gatagatatt gaacaaactg
    19321 gggaagtatt tatatcaggt attgagattg atatgattaa gaaagctcaa gaacttataa
    19381 ataatatagt agttgaacca gaagtaggaa aaacttataa aggtaaggta agtagaataa
    19441 tgaactttgg agcatttgtt gagatacttc caggaaaaga aggattactt catatatctc
    19501 atatagctca tgaaagagtt gccaaagttg aagatgtatt gaatataggg gatgaagttg
    19561 aagttaaagt tacagagatt gatgaaaaag gaagagttaa tttatcaaga aaagttcttt
    19621 taccaaaacc agaacataaa aataaataac ttattatata attaaaagca aagtcttagg
    19681 attttgcttt tttaaataca aaaaagtaga taattttaaa atgatagtta tggatggctt
    19741 atagttcttt ttctgtcagg ctttactctt ataaagaagc tatttaaaag ctgattaata
    19801 gagagcttta ttgatatata tagaatcact taagttatat ttagtgcata aacagagctt
    19861 atttttaata gtattaaata agaaacaatt tgtacataat tagggggagt tttaatgata
    19921 atgatggtac ttaataagag taagctcaaa aaaatcataa tactaatttt agtagtgtta
    19981 ttagtattat caggaggggt aatggtatta aagttattta acaacaagcc agtatttaat
    20041 ttatttaatg gtgtggattt accatatgaa agaggtacaa aggataaaag tggatatgtt
    20101 gcattaacct gtaatataga ccttggatgg gaaacagagt acgtagaatc cattttagaa
    20161 acacttaaaa aagaaaatgt aaagattaca tttaatgtaa ctggaaaatg ggcagaaaag
    20221 aataaggatg aattattaaa gataaaaaaa caaggacatg aaataggtaa tcatggatac
    20281 aaacatttag attattctac attatcatat gaagacaatt atgagcaaat agagacctct
    20341 aaaaagataa ttgaagaaat tataggtgaa aaaactaaat tttttcaagc accagcaggt
    20401 tcttttggtc cagaaacagt aaaggctgct aaagcattag ggtatacttc tatcaaatgg
    20461 gatgccgata ctatagattg gaaatacaaa gaccaaccag aggtaatcat tgatagaatg
    20521 aagaaaaaag acattaaaga tagtagtata attttaatgc atccaaccaa tgcaacgaca
    20581 aaatgtatag atgatattat agctattgta agagaaaaag gactaaaacc aggaaaactt
    20641 agtgatgtat ttaagtagac aaagtataaa tatttggtta agattgtata agttgtagtt
    20701 gaattaatta aaagactgac ttataatgta atacatataa aaaataaata taattaaaac
    20761 atagtgtttt caataatcca atatgaaaca aagaataaat tgttaggaga atgtaatgta
    20821 taagacaaaa atactggaaa atgggcttac aataattggt gaagaaatac catatctaaa
    20881 gtcaataaca ttaggtattt ggataaatgc aggctctagg atagaagaag cacaggtgag
    20941 tggaacttct cattttatag agcatatgat gtttaaagga accaaaaaca gaacatcaaa
    21001 agagattgct agttcaatag ataatttggg tggtcaaata aatgcattta caagtaaaga
    21061 atgtacatgt tattatgtaa aactaataga tgagcatata gatacaggaa ttgatgtatt
    21121 gagtgatatg atactaaatt ctaaatttga taaaaatgat atagataagg aaagacttat
    21181 aatattggaa gaacttaaga tgtatgaaga ctctccagat gacttatctt atgatttatt
    21241 agtggaaaat atatatgcta atgatggatt aggaatgaat ataattggga caaaagaatc
    21301 tctgtacaat ataactagag aatccatgtt agaatactta aataagtatt atattccaaa
    21361 taacgctgtt atttctatag ctggaaactt taactttgat gatatggtgg agaaaatcaa
    21421 atcaaaattt ggacactggg aaaagaaaaa tttaagtata gacataagtg aggctaagtt
    21481 taatccttgc tttatatcaa aaaataaaga tactgaacag gtgaacttag ctatgtgcct
    21541 aaaaggtatt ccttttgaaa atgatgaaga agtctattca atggcagtgg taaataatat
    21601 tttcggtgga agtataagct caaggctttt tcagaaaata agggaagaaa aaggtttagt
    21661 atattcaata tactcatcac agacattata taggaaatgt ggtgaattag gtatatttgc
    21721 aagcatgagt acagaaaatt tacaagatgt atataattta ataaagaaag aaattgaaaa
    21781 tataagagaa aactatctta cagaaaaaga aatatctgaa agcaaggaac agttaaaagg
    21841 aaattatata ttggatttag aaagtactag cagtagaatg atgtctactg gtaaatctat
    21901 gttacttagt aaaaaagtaa aaactacaga tgaaatactt gaatgtataa ataatgttaa
    21961 tattaattca ataaagaagg ttgttgataa ggtattcaat atcgaaaata ttggtacatg
    22021 tatagttgga agagatgtag aaaagattct acacttggat taatattgaa aaaagtaaat
    22081 ctctggaggt ataaaatatg aatttatctg aaatagcagg taaggaaata gttaacttgg
    22141 tcacaggaga aagattgggg gtcattggag aatgtgacct tatactagat gaaactacag
    22201 gcggtatatt agcgcttcta attccaaaag aaaaaggatt ttttgccaga agaaaagata
    22261 agtcattttt agaagtacca tggagaaatg taaaaaaaat aggtaatgac atgataatta
    22321 tagaatatga gggatatatt taggaggaaa aaatgagtat tttagtgcaa aagtttggtg
    22381 ggacatcagt agaatcatat gaaaaaatga atgaagtatg taagatagta aaagcataca
    22441 aaaaaaatga tgaagaatta caattagtat tagtagtttc agcaatggga agaaaaggag
    22501 ctccatatgc tacagataca cttataaatt tgtgtagtgc agtaaatgat gagccaagta
    22561 aaagagaact tgatttaata atgtcttgtg gagaaataat atcaggaaca attcttgcta
    22621 atttattgaa tgcacagggg atagattctg ttttctttac tggtcagcaa gcaggtataa
    22681 ttacaagtga tgaatattct aatgcaaaga taaagtatat aaatcctaaa aaggtaaaaa
    22741 gagctttaga tgatggtaag gttgtagtta tagctgggtt ccagggtgta acagatgatg
    22801 gagaaataac tactcttgga agaggcggaa gcgatacatc agcagttgct attggtaaag
    22861 ctttagaatg tgaaacagtt gaaatttaca ctgatgtgga tggtataatg actgcagacc
    22921 ctagagtaga accaaacgct aaagtattga gttttataga ttatgaggaa gtattccaaa
    22981 tggctgacaa aggtgcaaaa gtaatacatc caagagcagt acagttggct aaaagtggaa
    23041 atataacttt agccataaag aatacaatga atccaacttt tgaaggtaca aaaataggtt
    23101 ctttatgtag acacttagaa gacaatatag aatatgaaca agaaagagat tttaaggttg
    23161 ctgttgcaaa taaagatagc gtagcacaag ttaagattaa gtcagcagaa gaagtgttta
    23221 cagaagtatt aaatgagata gaaaagaaac ttataactat agatatgata aatttcttta
    23281 tatcagaaaa agcatttgtt gtagaagatg ctgatataaa atcattaaag gaaatactag
    23341 ataaatttga attagattat gaagtaaata gagactgtgc taaggtaaca ttgatatgtt
    23401 caagaattgc tgatgaaatg tctggtatca tgtcaaaggt agttagagga ttatcaaaag
    23461 caggggtatc tcttttacaa acatcagact caaatatgac tatatcttgt ttagttagtg
    23521 aagaagatat gcatacagca gtacatgcta tacaccaaca attttattta aaataaactg
    23581 tataatacaa ttatttttta atcaaattta agaatttcat atatatctta atatatttga
    23641 tttatttggt aaaactaaat taaatataat tttgatagga gtttttttat gaaagattat
    23701 agagaagata atgaaagatt tgataaagat aattgtagtg aagaatctag ttgcgaagag
    23761 agagaatgta taaagcaatt tggtaccaat gaaatgccac ctcaacctcc taaagatatt
    23821 caatgtataa ctataattgg agaaattgag ggacatttta taggaaatcc tcaaaaaaaa
    23881 gctacaaagt acgagcatat aattccaatg ctatactcaa ttgaggaaag taatgatgtt
    23941 aaaggagtgt tagttgtttt aaacactgtt ggaggagata ttgaagcagg tttagctatt
    24001 gcagaacttt taaacagtac atctaaaaaa gtagttactc ttgttctagg aggaagtcat
    24061 agtataggtg ttcctcttgc tacagcagga gattactcat ttatagcacc aacagcaacc
    24121 atgattatac atccagtgcg aactactggt cttgtgattg ggataaatga gacttttgag
    24181 tattttaaaa agatgcaaga tagaataatt caatttataa taagaacatc aaatatcaaa
    24241 aaagatgtac ttgaaaaact tatgcatgaa aaagatgaac ttgtaagtga tgttggtagt
    24301 gtattaatag gaaaagaagc tgtggattat ggtcttatag atgaagttgg aggactaaaa
    24361 gaagcactta aaaaattaag agagcttata aaagaatctg aggaagaaaa aaataatgat
    24421 taaaatttgt ttaaataata aaatttatga tataatgatg taataaagta ggagtgttag
    24481 tgttaaggca ttagcacttt ttgctgtgaa ggggtaaaac ttgctaaaaa taaggagtga
    24541 tattaatggc cacgaaaaag aagaaaaaaa agaaggtaaa taccttaagt tttaatgcag
    24601 aatatcataa tttaataaca atatttatag gtgtattttt attatatagt ttaaattcta
    24661 attctatggg atggatacct gtgttaatgc aaaatctttt taagggacta tttggagggc
    24721 tttcaatagc aataccattt atagtaatta taacaggact tttaggattt tttgatggaa
    24781 atgaatatat atataggcta agaaagacta aactatacta cataattatt ttatttatat
    24841 ttgtatttta tggattatta aatgcaggaa ctcttcctgt agatagccca ctaaaaggta
    24901 acatgtttga tgatgttatg aaattgggtg taagtggaca aggtagtggt ttgatagcaa
    24961 caactattgc gtattatatg agtaaaatat ttggtatagc aggtggatgg ttaataagta
    25021 tcttcgcact tatactttct gtaatgttta tatttaatat ttctataaaa gatttggtgt
    25081 caaatgcaaa atcaaaatct aaagcatcta aagacagcaa tttaacgttt aaagataaaa
    25141 ttgcaaatat gaaaaaatct gcaatagata tgatgactga tgaagtagat gatactacta
    25201 taaataaaaa accaggtttc tttaaagggt taatgtcaaa aggtcttaat aatgatgatg
    25261 aagaagatga atattttgaa gaagaaaata gtgatggtgt agatgataaa actataaaaa
    25321 tagttggatt taataaggca gaagatgagt atttagaaat attagaagga actcaaagta
    25381 tgccagagtt agatgtttta aaagaattac aaaaagctac caatgaaaat ccagttgtag
    25441 atactaaacc agaaaaaaaa gttgatatag ctaaacctaa tttaaatata gaaaaaactc
    25501 aacctatgag tattgtagca gagccagtta atgaggatta ttcaaactat aaaaagccta
    25561 gcattgagct tttaaataaa gtaaataaga agtctgatga aaatggtaaa aagaaagtgt
    25621 tgaaaaatgc ttctctttta gaaaagacac tatcagattt tggagtggaa gcaaaaatta
    25681 atcaagttac agttggacct actataacaa gatatgaaat acaaccaagt ccaggagtca
    25741 aggtaagtaa aattgtaaat ttaactgatg atatagcttt aagtttagca gctaaaagta
    25801 taagaattga agccccaatt cctggtaaaa gtgcaattgg aatagaagtt ccaaatgaag
    25861 aagctcaaat ggttggtgtg agagaagttc tagaaagtga agaatttaat aattttaact
    25921 ctcctcttgc aatgggactt ggaaaagacg tagcaggaaa aattataatt ggagatatag
    25981 gtaagatgcc acacttacta atagctggtt ctacaggttc aggaaagagt gtgtgtgtaa
    26041 atacattaat aagtagtatt ttatataagg caaatcctga tgaagtaaaa ttactattaa
    26101 tagacccaaa agtagttgaa cttgcaaatt ataatggaat accacatcta ttgataccag
    26161 tagttactga ccctaaaaaa gctgcaaatg ctttaaattg ggcagttaca gagatgaata
    26221 gaaggtacaa attatttgca gatgctcaag taaaagatgt aacaagttat aatgagaaag
    26281 cggaagaaaa acttcctaaa atagtaataa taatagatga gttagcagat ttaatgatgg
    26341 caagtgcaaa tgatgtagaa gattatattt gtagattagc tcaaatggca agagctgctg
    26401 gaatgcactt gattgtagca actcaaagac catctgttga cgttataaca ggggtaataa
    26461 aggctaatat accatctaga atagcatttg cagtatcatc tcaaactgat tcaagaacaa
    26521 tccttgatat gggtggtgca gagaagcttc taggtaaagg tgatatgtta ttctatccac
    26581 ttggtgcagc aaaaccagtt agacttcaag gggcattcat atcagagagt gagtcggaaa
    26641 aagtaatcga ttttgtaaaa agtcaagtta aagatggaat aaaatatgaa gaagatataa
    26701 ttgaaactat atctaaggtt aatacttcaa agggtagtga tgaggatgag tttttgagtg
    26761 aagcgataga atttgtcgtg gaaagtggtc aagcatctgc ctctatgcta cagagaagat
    26821 ttaaaatagg attcaataga gcagctagac ttatagattc tatggaagaa agaggaataa
    26881 ttggagcaag tgagggaagt aaaccgagaa aggtattaat aagcaaacag gatttgcaga
    26941 atttagaagg tgaatagaat tgtcagttat aaaaatagaa aaagcactta agctagataa
    27001 agtaatattt gtagatgtga gaactgaagg agaatatgaa gaagaccata tattgaatgc
    27061 aatcaatatg cctcttttta aaaataatga acataatgac gttggaacaa tttataagat
    27121 gcagggaaaa catgaagcaa tacaaaaagg gtttgactat gtatcgtata aattaaagga
    27181 tatatattta caagcagcag aacttgcatt aaattatgat aatattgtca tatattgtgc
    27241 aagaggcgga atgagaagtg gcagtatagt aaatttatta tcatcattag gtgtaaatgt
    27301 atatcaactt gaaggtggat ataaggctta tagaaatttt gtattagagt atctaaggca
    27361 tgtaatggat acaaaaaact ttgtatctgt tcatggattg actggtacag gaaagactga
    27421 tttgttacat ttattagatg aaaaaggaat agatgtactg gatttagaag gcatagctaa
    27481 aaacagtggt tctacctttg gttttataac ttttgataaa aagccacctt ctcaaaagaa
    27541 ctttgaaaca aaactatttg aaagtatatt tttttcgaaa gagaattata tatttgtcga
    27601 gagtgaaagc aaacgagttg gaagtgttac agttccaaat gaaattttcg aggcaatgac
    27661 acgagaagga catcatatat tattagaatg tgatattgaa aatagagttg atagactttg
    27721 tagagattat atttatgata aagatgaagg taatatattg attttaaaag aatgtataaa
    27781 caagtttaga aaaagattgg gtaataaagt agtagatgat tatattgatt tattagaatc
    27841 aggtgaatat aaagaacttg taaaaagata tcttttagaa tactacgacc cactttatat
    27901 gcattcagta gaaaaatata agtatagtaa gattataaat tttaatgata cacaaaaagc
    27961 tttagaagat gttatagact tatatgagag tatattggaa ggagaaagtg aatgttaaaa
    28021 atagcattag aatcattagg atgctcaaaa aatttagtag atgcagaaat aatgatggga
    28081 attttaaata acaaaggcta taaattaatt ggagattttg aagaagctga tgtaattata
    28141 gtaaatacat gtggatttat agagtcagct aaacaagaat ctatagatac gataataaac
    28201 tttgcagagt taaaaaaaac tggcaattta aagttgttga tagtaacagg gtgtctagcc
    28261 caaagatatt ctgaagaatt aaaaactgaa attcctgaaa tagatgctat agtaggaaca
    28321 ggaagctatc aaaatataga taaaatactt aaagaattaa gtgaaatgca taaaattgta
    28381 agccttaatg atatagagtt tgtatttaat gaagatttac caagatatat atctacacct
    28441 agctatatgg cctatttaaa aattggagaa ggatgttcca ataattgtac ttattgtata
    28501 ataccaaaac taagaggtaa atatagaagt agaaaatttg aagatataat taaagaagct
    28561 aagaaattag ctgaatctgg agtaaaagaa cttgtggtta tagcacagga tacgacaaag
    28621 tacggttttg atttatatgg aaaagaaaga ttatcagaac ttttagaaga acttgcaaaa
    28681 atagatggat ttaagtggat aagggttatg tattcatatc cagaatctat aacagaagaa
    28741 ttgatacaag ttataaagaa atatgataat atatgtagtt actttgatat gccaatacag
    28801 catgcaagta acaatatttt aaaattaatg aatagaaaaa ctactaaaga agatatatta
    28861 aataaaatca atctgataag aagtaatata ccagatgcta tacttagaac tacaataata
    28921 gtagggtttc ctggtgaaac agaagatgac ttcaaacaac ttgtggactt tgttgaagag
    28981 gttaaatttg atagacttgg tgcatttgca tattcaagag aagaagatac accagcagat
    29041 agattgccaa accatataga tgaagaagta aaaatacaaa gaagagatac tttaatgatg
    29101 attcaacaaa aaatatctga agaacttaat gacaaaaaga taggaaaaac ttatgaggtg
    29161 ttaatagaag aacagataga agataatgta tatacaggaa gaacacaagg tgatgcagaa
    29221 gaaattgaca gtatagtgta tgtaaaaagt gtagacaatc ttgaagtagg agaatttgta
    29281 agtgttcaaa taaatgatgc aatggaatac gatttaatgg gagatgtttt atatgaactt
    29341 gcctaacaag ttaactttgt ttagaatatt tttaatacca gtatttgttt taattatgtt
    29401 gctcaatata ccaaataaat ttttaatagc ttgtattatt tttataatag cttcaattac
    29461 tgatgcttta gatggaaaaa tagctagaaa atacaatcta gtcactgact ttggaaaatt
    29521 tatggaccca ttagcagata agttattggt tatttcagca cttacatgta tgatagaaga
    29581 ccatcttgta agtagttgga tggtaattat aatagtggct agagagctta cagttagcat
    29641 attaagagct atagctgctg ctgatggaaa agtaattgct gctggaaaca gtggaaaact
    29701 taaaacgata actcaaatgg tatctatagt gtttttattg ttaggtgctc agtttgaaaa
    29761 tgtattgata ttaaatattg gtgaaattct cattcttata gctacactac ttacattata
    29821 ttctggatgg gaatatctat ataaaaataa agagcttttt atgtcttcta agtaaaaaat
    29881 tatgctttaa taaataaaaa ctaatgatta ataaataaaa aataacaact taaaatttta
    29941 aaaatgtaaa aaagggtata ctattatagt atacctcttt tcattt
//
LOCUS       DAEOSP010000028        28547 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.028, whole genome shotgun sequence.
ACCESSION   DAEOSP010000028 DAEOSP010000000
VERSION     DAEOSP010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28547
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.028"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            45..395
                     /locus_tag="KRH54_001009"
     CDS             45..395
                     /locus_tag="KRH54_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBG8578678.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            414..2057
                     /locus_tag="KRH54_001010"
     CDS             414..2057
                     /locus_tag="KRH54_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBG8578679.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2138..4195
                     /gene="recG"
                     /locus_tag="KRH54_001011"
     CDS             2138..4195
                     /gene="recG"
                     /locus_tag="KRH54_001011"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBG8578680.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4254..4808
                     /gene="rsmD"
                     /locus_tag="KRH54_001012"
     CDS             4254..4808
                     /gene="rsmD"
                     /locus_tag="KRH54_001012"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBG8578681.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4810..5307
                     /gene="coaD"
                     /locus_tag="KRH54_001013"
     CDS             4810..5307
                     /gene="coaD"
                     /locus_tag="KRH54_001013"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBG8578682.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5311..5835
                     /locus_tag="KRH54_001014"
     CDS             5311..5835
                     /locus_tag="KRH54_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578683.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6179..8098
                     /locus_tag="KRH54_001015"
     CDS             6179..8098
                     /locus_tag="KRH54_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG8578684.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENIL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            8111..8398
                     /locus_tag="KRH54_001016"
     CDS             8111..8398
                     /locus_tag="KRH54_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8578685.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            8531..9790
                     /locus_tag="KRH54_001017"
     CDS             8531..9790
                     /locus_tag="KRH54_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBG8578686.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLMGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            9793..10533
                     /locus_tag="KRH54_001018"
     CDS             9793..10533
                     /locus_tag="KRH54_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG8578687.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            10856..12166
                     /locus_tag="KRH54_001019"
     CDS             10856..12166
                     /locus_tag="KRH54_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBG8578688.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            12264..13181
                     /locus_tag="KRH54_001020"
     CDS             12264..13181
                     /locus_tag="KRH54_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG8578689.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVRRDNSDF"
     gene            13197..14006
                     /locus_tag="KRH54_001021"
     CDS             13197..14006
                     /locus_tag="KRH54_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG8578690.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            14064..15812
                     /locus_tag="KRH54_001022"
     CDS             14064..15812
                     /locus_tag="KRH54_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBG8578691.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            16074..17096
                     /locus_tag="KRH54_001023"
     CDS             16074..17096
                     /locus_tag="KRH54_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBG8578692.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLYSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGITKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            17101..19407
                     /locus_tag="KRH54_001024"
     CDS             17101..19407
                     /locus_tag="KRH54_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBG8578693.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMCILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKKILDSKPSEKKEKSIDEFEPMEYI"
     gene            19379..20494
                     /locus_tag="KRH54_001025"
     CDS             19379..20494
                     /locus_tag="KRH54_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578694.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQKGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            20512..21930
                     /locus_tag="KRH54_001026"
     CDS             20512..21930
                     /locus_tag="KRH54_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBG8578695.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            21945..23153
                     /locus_tag="KRH54_001027"
     CDS             21945..23153
                     /locus_tag="KRH54_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578696.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(23227..24615)
                     /locus_tag="KRH54_001028"
     CDS             complement(23227..24615)
                     /locus_tag="KRH54_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBG8578697.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVIWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IGFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            24956..26290
                     /locus_tag="KRH54_001029"
     CDS             24956..26290
                     /locus_tag="KRH54_001029"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBG8578698.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREISYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(26432..27349)
                     /locus_tag="KRH54_001030"
     CDS             complement(26432..27349)
                     /locus_tag="KRH54_001030"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBG8578699.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            27557..28441
                     /locus_tag="KRH54_001031"
     CDS             27557..28441
                     /locus_tag="KRH54_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBG8578700.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
BASE COUNT        11147 a         3058 c         5092 g         9250 t
ORIGIN      
        1 attaagaaat gtttttaata aaaataagct taagggggtc aaccatgagt gcaaaggtaa
       61 cgaatcaata tggaaatata gaaatagata atcaagtaat agctcaagtc gtatatagag
      121 ctgcaatgga aagttatggc ttagtaggtt tagcatataa gtctaaagga atagtagagc
      181 tattaaaagg cgagaacgca actaaaggtg taagagttga ggaagtagaa gaagatgcca
      241 tagcaatcga actttatgtt ataatacaat atggtacaaa tatatctact gttgccaata
      301 atataataga tagagtaaaa tatgtagttg aaaaaatgac tggcgtaaga gttactagaa
      361 ttgatattaa tgtacaagga ataagagtta agtaatttca taggaggaaa caaatgattc
      421 aatatataga tggtaaaagg ttaagggaga tgtttatttc aggtgcaaac aatctccaaa
      481 acaataaaga gttagttgac aagttaaatg tattccctgt accagatgga gatacaggaa
      541 ctaatatgtc attaacaata tcttatgcct taaaagaatt agcaaaagtt gaaaatgata
      601 atatatcaga tatcggaaaa gcattatcta aaggttcttt aatgggagct agaggaaact
      661 caggagttat actttcacaa ataatcagag gtatagctaa atctatagaa ggaaaaagta
      721 aattatcaac agaagattta gcaaaagctt ttaaaaatgg ttcagacaca gcatataaag
      781 ctgtaataaa accaattgaa ggaactatac ttactgtagt tagagaaagt ggagagtttg
      841 caataaaaac tgcgaaaaaa gagaaagatg tagtaaagtt tttaagtatg cttgtgaagg
      901 aatcaaattc ttcattagaa agaactccag accttctaaa gaatctaaaa gaagctggtg
      961 tggttgactc tggtggaaag ggtcttgtac ttatatatga aggaatgtta gcttctataa
     1021 aaggcgataa catagaaata aaaaatgttg acctagatac taatatttca acaagtatgg
     1081 attttgcaaa gagtactact agcacagatg atataaaata ttgctattgt acagaattta
     1141 ttttagaaag ttctaaggta gaagatacta aaataagaga cataatgatg gcttatggtg
     1201 atagcctagc agtagttgga gatgatggag tcataaaggt acatgtacac actaatgacc
     1261 caggaaatgt acttcaagaa gctctaaagt acggtcaatt attaactata aaaatagaaa
     1321 atatgaagtt acaacatgaa aatacattac tggatgtaga agaaaagaaa gaaaatgata
     1381 gtgaaccttt agaagaagaa aaagagtttg ggtttatagc tacttctatg ggtgaaggtt
     1441 tggcaaatat atttaaagat ttcggagttg accatataat agaaggtggt caaactatga
     1501 atccaagtac tgaagatttt atgaatgcta taaagcatat aaatgctaaa aatatattta
     1561 ttttcccaaa taacagcaat ataataatgg ctgctaatca ggcaaaagaa ttgagtgata
     1621 aaaatataat agttatacct acaaaaaata cgcctcaagg atttgctgca ttagtaactt
     1681 ttaatggaga attaagcgaa gatgaaaata aagaagctat gatgaatgct ttaaactcag
     1741 ttaaatcagg tcaagtaact tttgctgtta gggatacagt gatgaatgaa attgatgtta
     1801 aagaaggtaa tataattgga attgcagaag gaaatttatt atcagcagga gattatgtgg
     1861 atgaagtaac atcaaacctt attgaaaagt tggttgatga agatactgct ataataacat
     1921 tattctttgg tgaagatgtt acagaatctc aagcaaatga actacgtact tctttagaag
     1981 aaaagtttga agatgtagat gtagagttat attatggagg acaacctctt tattattact
     2041 taatttcagt tgaataatga ttataagaac ctgtatgacc aagtagcttt tataaaattg
     2101 gttgtacagg atttttgtgt agtaggtgat atatttgttg gatttatata aagatgttca
     2161 atatgttaag ggaataggac ctaaaaaagc agataaatta aataaactgg gtatatttac
     2221 actaaaagac ctattgtatt attttccaag acaatttgaa gatagaaata atctaaagaa
     2281 aatagcacaa ttagaagatg gtgagaaagt tacgataaaa gcagtaatat caagtataaa
     2341 tacatttagc ccaaaagaag gtatgacttt aacaaagatt gatgtaaaag atgagacagg
     2401 ctcggctaaa ttagtgtttt ttaataagag ttatataaaa aatacttttc gtccaggaga
     2461 ttcaatatta gtttttggga aggttaagaa aaaatttaat aacttagaac ttacatcatg
     2521 tgaattagaa tatcttacta attcacctaa gaatacatgt agatttatgc ctgtgtacca
     2581 gcttacatac ggtgttacaa ataaagaaat tatgagtata attagaacag tacttgaaga
     2641 taaagaatta attatacaag aatatatgcc acaaagaatt atagaaaaat ataggctatg
     2701 tagtatagat tttgcagtta gaaacataca ttcaccaagt agtaaggaat cattaaaaat
     2761 agctttatat agaattgtat ttgaagaatt acttatacta caactagggt tgtttgtatt
     2821 taaaagtggc agaaataaag aagatggaat aaagtttgaa acaagtaaag acttaaaaaa
     2881 aataataagt gctttaccat ttaaactgac taaagctcaa aacagggcat tagatgaaat
     2941 tatacaagat atgaatttag agaaaataat gaatagattg gtacaaggtg atgtagggag
     3001 tggtaaaaca gtagtagctc tcttagcact tgcaaattgt gttttaaatg gttatcaggg
     3061 cgctttgatg gcacctacag agatattagc agggcaacat tatatttcac ttactgagtc
     3121 tctaaaagat tttggaataa atgtaggtct attaataggt agtcttacaa aaaaacaaaa
     3181 ggatacagta ttggaacaaa taaagaataa tgagattgat atattaatag gtacacatgc
     3241 actaattgaa gataaggttg aatttaataa tattggttta gttataacag atgaacaaca
     3301 tagatttggt gttatgcaaa ggagcaaact ttcattgaaa ggagcaaatc ctgatatatt
     3361 agtaatgaca gcaacaccaa taccaagaac tttagcactt atactctatg gagatttaga
     3421 tatttctatt atagatgaac ttcctccagg aagacagcca atagaaacaa ttgctattga
     3481 aaaaagtaag cgagatagag catataataa tcttgtaaga agagaggttg aatcaggaag
     3541 acaagtgtac attgtatgtc ccttggtaga agaaagtgaa gccatagaag ccaaatcagc
     3601 agttgaactt gttgaggagt taagagcgga atattttcac gacttaaggc ttgggttgct
     3661 tcatggtaaa atgaagtcaa gtgaaaaaga tgaagtaatg agacttttta aagataaaga
     3721 aatagatatt ttagtttcaa caacagtaat agaagttggt gtcaatgtgc caaatgcaac
     3781 attaatgata atagaaaatg cagaaagatt tggacttgca caattacatc agcttagagg
     3841 gcgagttgga agaggtagtc ataaatcgta ttgtgtacta atttatgatt ctaaaacaga
     3901 tgtatgtaga caaagaatgg ctataatgga agagaccaat gatggtttta agatttcaga
     3961 gaaggattta gaaataagag gacctgggga attttttggg acaaggcagc atggtcttcc
     4021 agaacttaag gtagcaaact tatttaagca tataaaaata ctgaagttag cacagcaaga
     4081 agctcgatat atattgggtg aagataataa tctacaatta aaagaaaaca tggcactcaa
     4141 aaaagaaatt atagataaat ttaaagatac attgaaagaa atttcattaa attaatttta
     4201 aattgtgtta aaatattact aatgtaataa tgtgctgaag ggagattgtc atattgagag
     4261 taatttcagg aaaagcgaga ggattaaaac taaatactcc aaaaaatgaa gatgtaaggc
     4321 caactacaga tagagtaaaa gagtctctat ttaatatgat aaattcatat ataatggaaa
     4381 gtgaagttct ggatttattt gctgggactg gttctttagg aatagagtgc ttatctaggg
     4441 gtgccaagtc atgtactttt gtagatatta gtaaggaaag catagatatt gttaagtcca
     4501 atataaaaaa ggcaagagtt gaaagtgaaa gtattatttt aaaccttgat tttaagactg
     4561 caatagataa acttaaatta caaaatagta aatttgacat aatttttatg gaccctccgt
     4621 attataaaaa tatgtttata gaagctattg aaaaaattga taattctaat ctattgaacg
     4681 aagatggaat aatagttgta gaacatgata cgaatgattt atttccagat aagatatgta
     4741 aattggagaa aactagagat aagaaatatg gaaatactac attaactttt tataagatgg
     4801 aggcataata tggaaaataa acctagaaaa gctatatttg caggaagttt tgacccaatt
     4861 actaatggac atttagatat tatttgcagg gcttcgaagt tatttgacga attacaaata
     4921 ggagtattaa ataatcctaa taaaaagggt ctatttagct ttgatgaaag agtaaaactt
     4981 atagaaaaaa gtacaagcca tttaaataat ataaaagttg tgacttttga tggtttatta
     5041 ataaattact gtcaggaaaa tgggattgga gcattagtta gaggtgtgag aagtggtgct
     5101 gatgttgatt atgaacttca gatggcacat atgaatagag agcttaatcc agatatagag
     5161 actataattt taccaagttg tacaaaatat tcatttataa gttcatcttt aataaaagag
     5221 gttttgctct ttgatgctga tataaaaaat ttggttccaa agattgtttt ggaagaatta
     5281 aagaaaaaaa ctagtggggg taattgatat atgaagatag atttagaaat gattgaacta
     5341 tttgagcagt tagaggagat atttagaagt gcttctacaa tccctttctc acataaatgc
     5401 acagtggata aagatgaggt tttagcaata attaatgata taagaacttt aataccagaa
     5461 gaagtaacac aggcagtatg gattaataaa gaaagaaata aaataataag tcaagctaag
     5521 caagatgcta caaatatagt tgaacaagcg aaaaaagaag ctgagagaat acaacaagaa
     5581 tatcaagata atatagagga attaaagaaa aattctgaag atgtagtaaa ggcatatgta
     5641 gaatccagtg aacctgttgt tcaagcagac caaagagcaa aagatatagt tgatagagct
     5701 gagagaatag ccaatgagat aagaattggg tcaattgaat atgcagagga tgtgctttct
     5761 agtgttgagt ataatttaag agaaatatta gaagaagtaa aaagagataa agctgaactt
     5821 agacctaaaa aataaatttt aaataaataa aaatggagct atcacacaaa aaattccaat
     5881 ttatgtgtga tagctctctt ttaatatagc aattttttgt tatataattg tgctttttag
     5941 attacttata tcttattttg atattataat gtagtttaat cttttatttg ttgaaatcaa
     6001 cttatttatt tgtataaata atataggtta ttttcaaatt tttttaataa ttttaccact
     6061 gtcttctttt agattagagt tagactgtgc aaaattataa aataatacta aatcatgtgc
     6121 aattatttgg tatggcacta catgtagaaa atatagataa taaaattaag gaggtagtat
     6181 gaaggaaagg gctaaaaaaa tattagattt tattattaaa aataacaatg taacatcaca
     6241 agaactacaa gaaaagttta atgtttcaaa gagaactatt tattacgata ttctagctat
     6301 aaatgaacaa ctaggaaaaa gtggtaatat aaaaaatgta aaacacaaat ttatatttga
     6361 aggtaattta tgtgatgcaa ggaaaataat ttcaactgaa gaagataaat ttttagatag
     6421 tgattacaga aaaactttta tattaaacaa gatattactt ggcgaaaaaa tatcaataga
     6481 aaaactaaca aatgaaatgt tactttcaaa aaacacagta gtacaaacaa taactgatgt
     6541 aaaaaaatac cttcaaacaa tggggttaag attagagtat aagggaaagt acaaaattat
     6601 aggtgatgaa tacgttatta gagagctgtt tctgataatt gttcaagaaa atatattgga
     6661 aattaattct ataagtgagg aagtaagtag tttcgataca aaaggacata taaaacttac
     6721 tgattattct ttattaaatc taactaagtt tgtggaattt ctaaataaaa ggataagaga
     6781 tgggaaaaca ttatactcat ataaatatct aaatgaagct aaaaaaatta gttactttag
     6841 taattgtaaa gaacttttat gtgaagaagc taatgaaaat gaacaagcct atatatgcac
     6901 ttacataagt tcacttccca gcttaaatag tgaagttaag gaagatgttg ttgaagaata
     6961 tgtagataaa cttatagata agtttgaagt aaatacagca ataaaattag aaagtaagca
     7021 tgagtttaag aaaaatattt taaggcacct ccattcttct tacaacagaa tacgatttaa
     7081 gtttcctatt agaaatccaa tgttagatga gactaagtat aaacatgagt cactttataa
     7141 aattataaaa tcaattatag aaaatgaaga agagtttcct gtatttgagg ggataagaga
     7201 agaagaaata ggatttatag cagcgtattt tggaggatat ttaagagggt ctagggataa
     7261 tggtctaaga agaaataaag tcttactagt ttgtcctaat ggtcttatgg tatcaaagag
     7321 tttggaaata caactatata aatacatacc aaccatagaa atagtaggta tagtatccat
     7381 taagcaacta aaagaagtaa atgtatacta cgattacata attacaacta tagatataca
     7441 aaatgtaaac aacgtaattg ttgtaaatcc tcttttaaca tcatcagatg ttcaattact
     7501 tatgaacaaa ctaataagtg taaaagaaaa cgagaagtat ttcaatcttg aattaataat
     7561 acaagctata agaaaaaatg gagttatcaa taatgaagaa gcactaaaag ctgatttatt
     7621 aaacataata cacaagatag atgaaggaga gatgtatcaa ccaatgttaa aggaattaat
     7681 taatgcagaa agggtcaata taataaaaag tgttagagat tggaaagaag caataaaaat
     7741 agcctcaaag ccattgttag aagataactc tatagaagag ttatatatag aaaatatgat
     7801 aaagtcagtt gagaaatatg gaccatatat tgttttagct gatagatttg ctctaccaca
     7861 tgcaagttct aaagaaggag taaacaagct tgctatgtct ttactaatag tagaagatga
     7921 agttgattta ttaggaaaac cagtaaatat atttatggta ttagctgctg tagataacac
     7981 tactcacatt agagcattag catcactatc agagatgatg tacgaggaag aaaatgtaaa
     8041 actaataatt aatggtgata aaagttcaat actagaactt ataaataagc aaaattagga
     8101 ggaatgaaaa atgaagatat tagcagtatg tggttttggt gtaggttcat caatggtatt
     8161 aaaaatgaca atacaaaagg ctctaaagca gttaggtata gaatgtgaag tagaaaatac
     8221 agatataaat tcagctagag gtgctgattg tgactgtata ttcacaagtc aagaattgtt
     8281 aaatgaatta aaaggaactt gtaatgtaga tgtatatcca gttaaaaagt acatggattt
     8341 agcagaagtc acagaagttg ttcaaacttt tttaagtgat aaaaatgata agaaatagta
     8401 taagttttaa aaagtaaatt taagttaatc agaataaaat aaaaacttac taaattcaaa
     8461 aatagtaaga aagagaggag ggatttagtg gtagaaaact taaacagttg acctatcact
     8521 aaaaaatata atgcttgatt ttatttcaaa taatatttta cgtaatccat ctatgttaat
     8581 tggattaata gcagcatttg gtcttgtact acagaagaag aaagcatctg atgttataaa
     8641 ggggtcacta cttgcagctt tcggaattat aattatggag actggaacag gtatgttagt
     8701 tagttcaata gcaccaatta atggagcttt ccaaagtata agtggtggtg cagtaacaaa
     8761 aggtttaagt gatgttactt ttactgcaag ttatggtgga attataggtt tagcaatgtt
     8821 tgcgggttta gctattcacc taatgatagc aagatttact cctattaaga caatcttttt
     8881 aacaggtcac atgctttatt ggttcccatt catatttgta gcttcaggag ttgaagctgg
     8941 attaaatggt ggagtattaa tagcttttgc agcagtatta tcagcactct attggtcaat
     9001 aatgccttgg ttattaagaa aatatgtgtt tgatgttata ggagatgatt cttttacatt
     9061 aggtcatcca tctggttgtt tagcacttat atcaggattt gttgctaaat tagtaggaaa
     9121 taaagaaaaa tctacagaag atataaacat acctcaaagt ttatcatttt ttagagaaat
     9181 atctataagt ggtggaatgg cagtgttttt aatgtttata gttgttggga tagtaatacc
     9241 aggtgcgttt aaagatagta atgagtttat tggaacgtat gcaataaagc aaggtctttc
     9301 ttttggagca ggtcttataa taatgcttca tggggtaaga atgctaataa atcaaataat
     9361 gccagcattt caaggtatat ctgaaaaagt tgtgccaaat gcactacctg cttttgactg
     9421 tccaatatta tttaattata aaccaaatgc tgtattaatg ggattcttag ttgctatgct
     9481 aacatctaca gctatgattt taatatgtaa ctactttaat gtatttaatt tgctacttat
     9541 accacttgtt ataacttcat tctttgagtg tggagcagca gcagtaatag gagaatcaca
     9601 aggtggtata cgtggagcta taataggtac agctatagct gcaatattta tggtagtttt
     9661 agtaggaata tctgctgtaa tttactcagg aactattcaa aattggatac taatatttgg
     9721 aggtaatgac ctttcattat ttggtataat cagtaggttt ataggttcac tatttggagg
     9781 aatattatag atatgaaatt taaatacata gatgagataa gaagattaat tgatataata
     9841 gaaaacgaag aaaaagtcaa tatggaaaaa gcagtacaaa tctttgttga agcaatagaa
     9901 agtaaaaatg ctatattttc atttggagct tcacatgcag gaatattaac agaagaatta
     9961 ttctatagag caggtggctg tgttcttata aatccagtat ttgcaaaatc tctaatgtta
    10021 gatacagcgc ctataactca cacaagtaag atggaaagat tagaaggata tggtacagct
    10081 ctagctgata aaactccaat taaaaaagga gatgtagtta tagttcattc agtatctggt
    10141 agaaatccag taagtataga gtttgctatg aaagctaagg aaaataaggc aatagtaata
    10201 tgtataacta atttaagtta ctcaaaaagt gtatcttcaa gacattcttc aggaaaaaaa
    10261 ctttatgaag tatgcgattt agtgatagat aatcatggtc aaaagggaga tgcctgtgta
    10321 gagatagatg gattaaacca aaaagttagt ccaacttcta ctgtaatagc aactactgtg
    10381 atgaactcta ttatagctca agctactcag gaattagtta ataaagggct aaagaatcct
    10441 ccgattttct atagtgcaaa tattgatggt ggagatgagt taaataagaa gatttttgat
    10501 gaatataaaa gtgtaattca ttatgaatat taaggttaaa aaaatatgtt aatgaacttt
    10561 gaagtttatc aacaaagaat attaataata tataataaat gctatcattt aaaagctgta
    10621 ggtaaaaatc tacagctttt tattattgta gattaaaaag atgcgtaatt aaattttaaa
    10681 ttggagaaaa taaattggaa ggtatcaatt ataaatatta gaaacgttgt catagaaaaa
    10741 aactatatta aaaaaaagta catagaaaaa actgattaga caataaatac atgctttgat
    10801 aatattttaa atggagtgaa aatgtgtaga attataatga aaaaagaaaa taaaaatgaa
    10861 aaaagaaaat aaaatggagg gaataaaagt gggtatgtta aaaaaattat gttcactagc
    10921 tatggtagca attatgacta caacacttat aacagggtgt tcaagtggaa atggaaaaga
    10981 ttcaaaaaag gatggcgaaa aagaaaaact agttctttgg atgccaccag tagaaaataa
    11041 tatgaaggaa gtttgggacc ctattttaga taaatttgag gaaaaaaata actgtgaagt
    11101 agatttacag ataattcctt gggataatta ttctgaaaaa tttgcaacag ctataagtgc
    11161 tggcgaaggt ccagatgtag gatatatgta tgcagaaatg tatccacagt ttatagaaag
    11221 tggagcagta gaagatttga ctaattatgc aactaaagag gataaagagc aatctattta
    11281 tataaaaaca tctgaaatga tgggtggaat gtatggtatg ccattccaag cagcaaatcc
    11341 aggggtatta tactataaca aggatatttt agagaaatta ggagaaaaac ctcctaaaac
    11401 atgggaagat tttaaaagaa tttgtaaaaa agcaacacaa gatactgatg gtgatggaaa
    11461 gatagaccaa tggggattag ctcagggatg gggagctaaa acttttggta acttaaactg
    11521 gaactggtat ccttatttat ggcaagctgg tggagatatc tttaatgatg atttaaaatc
    11581 ggttaaattt aatgataaga gtggtttaga agcagctaat ttcttaaaag aattacaagc
    11641 atatgtgcct gaagattctt tatctaaaga tagtaatgaa atgatagaaa gtgtatttgg
    11701 accagggaaa gcagcattta caataatgtt atcttctgct gcaacaagtg tatttgatga
    11761 atcattcccg gatttaaatt ggggatttgt aactggacta gaagataaga aagcagcaac
    11821 ttttggagca gtagaccatt tatctttaat gtcatctgct aaaaataaag aacttgcata
    11881 caagttaata caacatatgt taagtgttga atcaatgaca gaatttcata aagcaatacc
    11941 aagagcacca ataacaaaag gtgaaccata tcaaggtgat gaaagattca aagaaatggt
    12001 ggaaaatgac aaaaatgtat atagaccatt agtagtagga cctcatggag ttgaaatata
    12061 tgaatattta tggaaagaac ttcaaactat gatttcagga gataaaactc caaaacaagc
    12121 gttagatgat gctgcaaaat attcaaatga tttattagca caataaaagt ttaaagctgt
    12181 aatatgttgt aaaggacttt acagattaaa attagcattg tatttataaa tacaatgcta
    12241 atatttatat tggagaggat aaaatggaaa acttagttag agagtttgaa gaaaattcaa
    12301 aaagaaaaaa aacaaaatta aatattaaac catatatgta tatattgcca ttgggaataa
    12361 ttttagtggc gttttatgtg ataccaataa taatgtcaat atactttagt tttacaaagt
    12421 ataatattat tagtccagct acttttatag gtttagaaaa ttataaaaaa ttatttacag
    12481 atgaaacatt aaaagtatct attataaata ctattaaatt tactgttgtt gtagtacctt
    12541 gccaaactat attatcattg attttggctg tttggattac aggaaaagga aatagtaaaa
    12601 tagcttcatt tgctaaaggt gctatattta taccagtatt atcatctatg gttttgatag
    12661 gtatggtatg gagggcttta ttgaatggag aaggttctat tatatatcag gtattaggtg
    12721 cctttggaat agaatcatct aaacttttag gagatagtaa gacagcatta cctacactta
    12781 tgtttatatc tatgtggaaa aatatagggt atttcatggt aatttatatt tcagctataa
    12841 tgaatttacc aaaacactgc tatgaagtag cgaaggttga tggggctaca aaacttcaag
    12901 aatttataaa aataactgta cctttattga aaccaactac aataatggtt gtatttttag
    12961 ggtcgatatg gtcattacaa gtatttgact taatttatac tgtgacaggt ggaggtccag
    13021 gtatatctac tatgagtata gttatgcatg catttaactt aaactttaaa aactttaatt
    13081 ctggatatgc aatgactgta gcaaacgtat tatttttgtt aatagcagta gtttcgattt
    13141 tacaaaataa gcttgtaaga cgagacaatt cagattttta gaaaggtagg tataatatga
    13201 aaacagcaag aagattatta agtgatggtt tacttttatt tatagcgtgt gcaagtttgg
    13261 taccttttat atacatgttg ataatatcat tgaagataac atataactca tatagtttgg
    13321 atatatcttt ttcaacagta acccttcaaa actatataga tatttttact aaaaaaggat
    13381 ttgcacaata tttttttaat acagcaatag tatctttttc aggtgtatta ttaaatttag
    13441 tatttagtac attagcagga tattcttttg cgaagatgga ttttaaaggt agtgataagt
    13501 tatttttatt tatgataatg actttaatta taccttccca agttacaatg ataccactat
    13561 atataataat gaaacactta ggatggataa atagttatct tgcacttatt atgccaatac
    13621 caactgcatt tggcgtattt ataatgagac aagctatttt aggagtacca aaagagttat
    13681 tagaatcagc aaagatagat ggttgctcgg attttagaat tttaatacaa atagtgttgc
    13741 ctttaattaa accagcatta attacactag caatatttac ttttatggga gcatggaacg
    13801 agtttatgtg gcctcttata gctacaacta aagatgcaat gagaacatta acagttggat
    13861 tatctactct taagacattc cagataacta attatggaca gatgatggca ggggctacaa
    13921 taacattttt acctccattt attttctatt tgattttaca gagtaaattt gttgaaggtg
    13981 tgtctttgtc tggtataaaa ggttaaatag tctaatttaa taatatataa agaaacttat
    14041 ataaatatgg aggaatttaa actatggaaa tttcatatgt actgaaagat tgcacattca
    14101 aacacaataa atactcagtt gtagaagctg aaacttggaa agataaggat ttggatattg
    14161 tagtagctaa aggtgaaaaa tttgcatttc aaattatgtt aaaagcaaca gaagaattta
    14221 attgtacaat tgataaaagt agtgctatat catggaaagg cctgggaaat agagtaaggt
    14281 tagcattaaa tatgcctaat agtttagaaa ataattttag tataaatata ttaggatatg
    14341 ttcaagatga tacaaaggca tttgtgaatg atgctatttt aagggataaa gatgtgttag
    14401 tagagcaata tttgcctcaa acattttgga ttgaaggtca agtaccagaa gattttgaag
    14461 aaaataacct tgatataggt attgatatat ttaaaagctt tggatatgaa gacgaagaaa
    14521 aagtttgtac aattgatgtt cctgtaaaag taagaaatgt tgtgctaaag cctttagatg
    14581 aaagcaagtt ctttttagat ttatggcaac atccaagctg tttagctaga atgtataaag
    14641 ttgagttatg gtctgatata cattttgaaa tagtcgataa ctatttaaaa gaattagcat
    14701 ctttaggaga aaaagttgca acagttatag tttcagatta tccatgggca gggcaaagtt
    14761 gttataaagt ttataaaaat ccatcaaact tatatgaata taacatggta agtgtatcaa
    14821 aaggattgga tggaaagatt aaatgtaact ttgagagtat ggataggtat ataagcatag
    14881 ctgataagta taaaatggct aaggagatag atttatttgg tctattaggt aattggtgtg
    14941 ctggtgagtt tggaaatcct gtagaaggtt ataaggaccc tataagagta agatattttg
    15001 atgaaaagga taaagtattt aaatttataa acaatacaaa tgatttaaaa gaatatatag
    15061 gattagtatt aaatcattta atagagtgtg gattatggga cagagtaagg attatagctg
    15121 atgaaccaaa taatccagaa gtagtcaaag aatgtataga atttataaat tctactgtag
    15181 gtactcatca agttaaatat aaatcagcaa ctcacgacca aaacttttta gacagagcta
    15241 aagatgaaat tgatgatatg tcaataaatt taaaacttac tatacaaaat tataaagata
    15301 tagaaagtct taagaaaaaa atcaatgaca agggtgggat attgacttgg tttgtatgct
    15361 gttttccaga gaaaccaaat agttttttaa gttctccttt tgtagaaaat agaataatag
    15421 gatggtatac ttattacttt ggtcttgatg gatttttaag atgggactat aatttatgga
    15481 cagaagaccc ttggaaagat tcaagttaca aattccctat ttggaaagct ggagatatgt
    15541 tctttgttta tcctggaaaa gatttaaagc cagttagaag tgtcagaatg gagaatttaa
    15601 gatttggaat acaggacttt gagttattta caatgttaga aaaagaaaag ggaagagagt
    15661 atatagaagt tgagcttatg caagaattat taaataaaaa agaaaatgct gaaattaaag
    15721 gttttggtga catagaacta ggatattcac ttgataattg gggatatgat aaagttaaaa
    15781 aatatgtatt agagttgtta gacagagtat aaaaaatata agagtgtctc aaaatgaact
    15841 ttttagttca tgaggcactc tttttgtatg aaatcaatat agtttcattg tttcaacaat
    15901 gaaaaatagg attatttcta ttctaagaca gccctttagt tttttagttg tattcaatgt
    15961 tttaaaacta actataaacg ctataaagtt tataaatgac aataattata tataacatga
    16021 tgtactttaa tttgctataa tagggattga ataatattat atggaagata acattgtttg
    16081 gggggattaa tataaaaaga ataggtatta ttataatgtt ttttttaagt atatctttta
    16141 ttggatttta tcttgtaata agagataaca gtgatacagt taagatgcaa cgagggataa
    16201 atataggtaa tgcactagaa tcacctaagg attttccgtg ggatgtaaaa atgtcaaata
    16261 agttttttga tgatataaaa gatgcaggtt ttgacacagt acgtatacca gtaaggtttt
    16321 ctgattatac gtcagattct gataatttta aaattgatga agaatttttt aaaaagattg
    16381 ataaatatgt agattatgca ttggataaag acttaatagt tgtgttggat ttacaccatt
    16441 ttgaagaaat tatgaaagaa ccaagagttc ataaagaaaa gtttttaaaa atatggcaac
    16501 agatagctaa taggtatcaa aaatatgata aaaaattggt atttgaatta ttaaatgaac
    16561 ctaaagaaaa tttatattca cagttactaa acgaatacat tgaagaagca ataaagataa
    16621 ttagaaaaac taaccctaaa agaactatta tagtaggacc atataatttc tatcaaattg
    16681 attatttgaa tgaattaaat attcctaaag attctaatat agtagtttcc tttcactatt
    16741 acgagccaaa tgattttgca tttcaaggaa atatatatca taagggattt gagcacttaa
    16801 gcaatattac ttgggaagga acaaacgaac aaatggatta tttaaaaaaa aggtttgata
    16861 ctgtggaaaa ttgggcaaat aaaaataagg taaaaatctt cttgggtgaa tttggaataa
    16921 caaaagaggc accagaagct tcaagaagag catggataaa agctgtaaga gaagaagctg
    16981 aaaaacgaaa cttttcatgg gcttattggg agcttgcatc tggatttggt atatacaatc
    17041 aaattgaagg gacttgggat agagatatat taagtgcatt aatagaaaag aggtgatttt
    17101 atgaagaaaa attcaaaaaa gatacttata actttaacta ttattactaa tgtcatatat
    17161 atattgtgga gaatttttta cacagtacct aaagaagaag ggaagtttgc cttaatatgt
    17221 gccataatac ttctttttgt agagataatg ggcatgatgg aaatgtttgt gcattattat
    17281 ggtatgtcaa atattgagta ccctgaaaaa ccaatcataa gtgaagaact ttatccacat
    17341 gtagatgtat tcattgcaac atataatgaa tctgttgatt tggtgagaaa aactgtaaat
    17401 gggtgtattc atatgcagta tccagataag aaaaaggtac atatatacat atgtgatgat
    17461 ggaaatcgtg aagaaatgtg tattctagca gaaaaaatgg gtgtaaatta cataactaga
    17521 actgaaagag aaggagcaaa agctggaaat ttaaataatg caatgcaaca tacaaattcc
    17581 cctttgattg ctacatttga tgcagatatg ataccaatgc atgacttttt aattgctaca
    17641 gtaccatact ttttgaaaaa tgagcaagct aaaaaagatg gagaaaaaga ggagtatgaa
    17701 aaggttggtt ttgtgcaaac tccacagagt ttttataatc cagacttgtt tcaatttaat
    17761 ctgcactctg aaggaaggat tccaaatgag caagattatt tttacagaga cattcaattg
    17821 gctagaaata ggacaaattc tgtaatttat ggtggtagta atacagttat ttcaagggaa
    17881 gcacttgaag aagttgatgg attttatact tattctatta cagaagattt tgcaacaggt
    17941 atcttaattc aaagtaaggg gtatagatgt tatgctattc cagaagtaca tgcatcaggt
    18001 ttatctccta cagatttaaa aagtcttatt aagcaacgtg aaagatgggc acgtggatgt
    18061 atacaaactg gtagacgtct aaatattttg tttaggagag gtttaggttt ttggcagaaa
    18121 ataagttata tttcttcaat tacttattgg tatgcaagta taaaacgctt tgtatatata
    18181 atggcaccta ttttattttc tgtgtttaat gtgattgtag taaaatgtac gttacttcaa
    18241 gtattggtat tttggttgcc aatgtatatt ttaagtagtt tatctcttaa aatattttct
    18301 caaaatataa gaaatactag atggaccaat atatatgaaa ctataatgtt tcagtcttta
    18361 atgccagcag taatacttga aacgtttgca atatctaaaa ataaattttc agtaacaaat
    18421 aagagtaaat tagaagaaaa tagaatgtat aagtttttac agggaatacc ttattttatt
    18481 tacatggtct tatctatcat aggaatttta aaaatgtttg tagcaatatt taaaatgagt
    18541 tcaatgacat attcagttgt attattttgg ttgattggaa acctttttaa ccttgttatg
    18601 gctacattat ttatatcagg gagacagcag ttaagaaagt ctgagcgtta tattgctgaa
    18661 attgatttta agttaaagca aaattcttat gttctttctt caaaaacaat tgatatatca
    18721 gaaaatgggt ttgcattttt gctagaaaat ccagaatata tttccccaga agaagagttt
    18781 gaggtagagt ttagagaaaa atctggaaat gaaatgtata tagctaatat gaaagcaaag
    18841 attgttaatg ttgtggaagt aaattcaaag tggaaatatg cagcttatat tacacatatt
    18901 gaagatagtg aaatagataa ttggatgtgt attgttcacg atagaatacc tactttacca
    18961 atgacaatat ctaatcaact aggtttcttt gatgatttgc aaattaatgt aaaaaaacgt
    19021 attgaaaaaa ctagaacatt atcacgaaga agtccaagaa tcaatatgaa ttttcagatg
    19081 gatataaaaa atacaggtaa attaagaata gtgaatttta attatcaata tgtattatta
    19141 aattttgaga ataaaagtat ttatcctaaa gaaatagcat tggaaattaa tgaaaatatt
    19201 gtgttagaat gtgatttgtg tgagggtaaa attgatgaaa gaggtatctt atataaagta
    19261 aataacattg acagcataat gcaaaatttc tttttaagag atgaaatgat ggattggata
    19321 ttacaaaata agaaaattct tgattcaaag cctagtgaaa agaaggaaaa aagtatagat
    19381 gagtttgaac ccatggaata tatttaggag agatgaaatg aagactttaa aagtgttttt
    19441 ttgttctaca aaatatctta ttttggtgtt tatttttttt ataaatacta cttttataag
    19501 ttcagcaaca agtgataaaa ttttagattt atcctttaaa aagatagaga ctgacttaag
    19561 ttctaagatt acatatgagg atacaggtgt aaaaatagaa acggatagtt ctaaaagcga
    19621 taaggaacgt tatttatata tttaccaaaa tataaaagaa aattggagca tgtataataa
    19681 tttttatatt gaaattcaaa ataaaaataa aagttctcag agaataaatt taagcataca
    19741 aagtaaaaat atgcttgaat ttagattaaa agaaggtagt gaagtatttt tagaaggtaa
    19801 aaatataata tattcagata aaataaaaga aggatgcata gaagtaccag gtgaatttga
    19861 aggcaaaatt tatgtgaact ttaatagcct tataaatgag gaaagtaatg ttgtgctgga
    19921 ctcaaatatg ctttcaaaca ttgttagttg gggaattacg tttattccat ctgatgaaga
    19981 acataatatt gttattataa aaaagatatc tttattgtct gaagagaaat taagattttt
    20041 aaataatatt aaaataattg gcgatgaaga agtacaaata ccagtattgg gtcaaagtat
    20101 aagtcagtat gaagtattag gtcttaaaag tgattctaaa attaagtact ctcttatggg
    20161 aaaacaagat aatgtaagca tatctcaaaa aggtaaatta acattaagta ataaagccaa
    20221 acctggacaa attattttac aagttaatgt tgatgataag tttaaaattg gaaagaaaat
    20281 aacattaact gagtcttgga gtgttaataa aaaagataag gatggtgttc catatacatt
    20341 agtatatcct gagcaatcac caacagtaca agatatgaaa aaaataaact ttatgaataa
    20401 catcataacg tttgttcgaa ttttatttgt atctctagta ataatatgct ttggaatata
    20461 tttatattgg aaaaaatgtt caaaaactaa gtaaagagag gaggttagat tgtgttattt
    20521 tcaagtttaa tttttttatt ttatttttta cctataactt tggttttata ttatgtattt
    20581 aggtttaacc gaacgataca gaacatgata ttgcttgctg ttagtttatt tttttatgct
    20641 tggggagagc caaaatttgt tgttatcatg atagtttcca taataatgaa ctatatattt
    20701 ggtttactgg tagatagata tagggaaagt aaaataaaag taaaagtttt tttagttttg
    20761 atgtgtgcat acaatatagg tgttttattt atatttaaat atctagcttt tgcgctaaga
    20821 aatattagtg aacttattag tactgaattg actataccta gtattgtttt acccatagga
    20881 atttcatttt ttacatttca gggaatgtct tatgttatag atgtatatcg tagacatgga
    20941 gaagtccaaa agaacccttt ttatgtggga ttgtatatag catttttccc acaattaata
    21001 gcaggtccta tagttagata tgagagtgtg gcagaacaaa ttctcaatag aaaagaaact
    21061 tggaataaat tttctattgg gacttgtagg tttataacag gtctaggtaa aaaagtatta
    21121 atttcaaata acatggctat agtagcagac tatatttaca ctatgaatag tcaaggagaa
    21181 atagctgcat cacttgcatg gcttggttca atagcctata ctctgcaaat attctttgat
    21241 ttttctgctt attcagatat ggctataggc ttaggtctta tgtttggttt taaatttgaa
    21301 gagaacttta attatccata tatatcaaaa tcaattagtg agttttggag aagatggcac
    21361 atttctttgg gaatgtggtt taagagctat atatactttc cgcttggagg ttctagagta
    21421 gcaaataaag atattatgat tagaaatatg tttatagtgt ggttatttac tggaatatgg
    21481 catggagcag aatggacatt tgtaatttgg gggattttaa actttgtttt cctaattata
    21541 gagcgattta taatgtttga aaagattgaa aatcacaatt ttataaaaca tatttatgct
    21601 ttattagtag ttaattttgg gtgggtatta tttaggtcac ctaatttaaa agaagcatat
    21661 aattatttta aagctatgtt tggaggcaat ggagctatat ggagtgatta tacatatatg
    21721 tttttaaaag aatactgggt tttctttata tttgcattta tttttagtgt acctattgca
    21781 aagaaaatca ataagtttgt tgttgaacaa gctaaatata gtatgatatt taacgtattt
    21841 tatccaatct cagctatttt attgtttttt atttctgtga catatttagt aactggaagc
    21901 tataatccat ttatttattt taatttttag ttgggaggaa aagtatgaat agatttttta
    21961 aagtaagatt aataactgca attatgttct taattatttt attcgctttc tctatcttta
    22021 atgtttgtta ttctggaaaa gatacgatac aggaaataaa aaattcaata gctgaaaaaa
    22081 gtagtttaaa agatacaata tcatctattg ataatacagt gaatgacaaa gtaatataca
    22141 aaaatacgtt cattgaaact tatggctatt tacaaaaatt aatgggtaaa aatgagtttt
    22201 caaaatttag tgttgtaaaa gatacttctg gaaagatgca ttatacatat tttgctaaag
    22261 ctccaaatcc agttggtgtg atagcagata gggtaagaaa attttctgat gaaatggaaa
    22321 agcaaaacac taaattggtt tacctaatga ctccagataa gtatataaaa ggagtgacaa
    22381 aatttccaaa aggaatacct tattcttata gtaatgagac agcagataat tttttaaatc
    22441 aactaaaaaa gtataatgtg gactatattg attttagaga aaatatttta aagagtggta
    22501 ttccaaaaga tgatttattt tttaaaacag accatcactg gaaagtagaa acttcttttt
    22561 gggcatttgg agaattagtt gagcaactta ataaaaaata caatatgaat ttagatgaaa
    22621 atcattatta tagagataag gacaattata attcaattgt atatcctaag tcatttcttg
    22681 gttctatggg aagaaagact ggtatcttat atggtggtat agatgatttt acattaattt
    22741 atcctaaatt taagactaat tatacttatt atacagattc taaatctcaa aaatttgaat
    22801 taaaaggaag gtttgaagag agtttaatat tatcatatcc ttttaatgca gaccttgatt
    22861 taatggatgg tcaatcagat aagtatttta catatttatt ggggaacaga ccattagtta
    22921 aaataaaaaa tatagaaaat ccagatggat taaaagtgct gtttgttaaa gattctctaa
    22981 ttgtaccaac agcagcattt ttttcctctg tttgttcatc tatagatatg atagacccta
    23041 gatattatga tggagatata ctagaatatg caaaatccca tgactatgac tttgttttta
    23101 tatctgtata tccccaaaat cttacaaaag aattttttcc gttttgtgag taaatttcta
    23161 aaaaagtttt ctgataaaat aaatggctaa ctggtaaaaa gttagccatt tattttgagt
    23221 gttattctaa gaaatatttt ttttactaaa tacttcttta tctatttttc caagtgattt
    23281 tcctacagta attgatgata ccatagcacc attaacattt atcatagtac gtcccatatc
    23341 tagtataggg tctattgcaa gtataccacc aactaatgga aaataagaac caagaccaac
    23401 tccagagata acaactgaaa cggccattgt agcagttcct ggaagaccag ctattccaaa
    23461 cgaactaatt gttataacaa ttaacaacat aacataaaag ctcatatcca tgtttactcc
    23521 agccatattg gcaagtgtta cagccattaa agcaggatat atacctgcac aaccattcat
    23581 acccatattt gcacctaaac ttgctgtaaa gcttgataca ccctcatcaa ctccgtgatt
    23641 ttgtactaga ctttctatcg ttacaggcaa tgttcctaaa ctagaacgtg aagtaaatgc
    23701 cagaagtaaa gcttcagttg aattttttat ataagtaatg ggatttagac catttaaagt
    23761 tataaaaatt aaatgtataa taaataatat tgcaactgat atataaagag ctaatataaa
    23821 ctgtaaaaca gatactattg aactaatacc ttgtgaagtt attgtatttg caagcagagc
    23881 aaccacagca taaggcataa atttaataac agtcatagac acacttacaa ttattttgta
    23941 agatgcttct ataaaaccta taaatggttt tattgtttca aaatgttttt tacttaatct
    24001 ctttattgag gtagctataa atgtagcaaa tattattgta gctacaatat ttccatttgc
    24061 catagccatt acaggattag atggtagtaa tcctcttaaa gtatccacaa gagagtttat
    24121 ttctttaagt tcttgatttt caactaaagt aggcatatta gctcctacac ctaacttcat
    24181 tatatttgca acgataattc caacaattgc agctatagca gtagtagcaa gaagcatacc
    24241 taaagattta aatgtcagag ttccaagatt gttatctttc atatttataa ttacttttat
    24301 aatggataag aaaactaaag gtactactaa catttttaat aagtccataa atccagaacc
    24361 aaataggcta taccacttac ttacttcttg tagccaaatg acttgagttg tatcattagg
    24421 aaaaccagca aaagcttgta ttatgattcc caatatgagt cctagtactg tagcatatat
    24481 tgttctcaat gaaaatttta tttttttctt ttcaaattgt ctaacaataa agaacgtacc
    24541 tagaagtaat attatgaaaa aaattgtttt aaaatctgta atcataagaa attgtgataa
    24601 aaatgaactt tccaatattt ttctcctttt atttaaaaat aaatttttct aagtttaaat
    24661 gatatcaata tattaaaaaa atgtcaatat tatatcacaa tttacttaaa tagagtaaaa
    24721 caagttgatt ttgaaaaatg tgattaagta cagaaaaata gggtataata aatgtttaaa
    24781 gaatttgaat tgtggtaaga aaataaaaaa tagcttttat aagatttgat ttcaaattaa
    24841 aatgtgttaa ataaataact aaaaattatt ataaaaaatg ttttcataga atatatatca
    24901 aatgaaaagc ttaaaataag tgaagaaatt aactagacat caaaggagag ataaaatgag
    24961 agtaataata attggtggtg ttgcagctgg tatgagtgct gcagcaaaat taaaaagaat
    25021 aaaacctgag tatgaagttg ttgtttatga aaaaactgaa atagtatctt ttggtgcttg
    25081 tggattacca tactttgttg gtggattttt taatgatgct gatgaattat tagcaagaac
    25141 accagagaaa cttagagaag ctggaattga tttaaatata tttagagaag tcgttgaggt
    25201 tgatagtgaa tctaagaaaa taaaagtaaa aaacataaaa acagatgaaa tatatgaaga
    25261 ttactatgat aaattgatga tagcaactgg agcaagaagt attatgcctc ctataaaaaa
    25321 cataaaatta aagaatgttt ctacattaaa aagtttatat gatggagaat atttaaaaaa
    25381 acttttaagt aatgaagata ataaaagagt gacaataata ggagctggtt ttatagggtt
    25441 agaagcagtt gaggcatgta agaaattagg taaagatgta gatgtaattc agttagaaga
    25501 tagaatattg ccacaagtat ttgataagga aatgacggat gtattagaag aagaaataaa
    25561 tagacataat gttaatttgc acttagatga aatggttgta gaactttgtg gagaagataa
    25621 agtagaaaaa gttataacaa acaaagggga aatagataca gatgtagtaa taattgctac
    25681 tggcgtgaga cctaataccg agtttttgtc aaatactaat ataaaaatgc taaaaaatgg
    25741 agctatagta gttgatgagt atggaagaac ttctgtagaa gatatatact ctgctggaga
    25801 ttgtgcaact ataaagaata tagtaagtaa tgaaaatgta tatgttcctt tagctactgg
    25861 agctaataaa ttgggtagga tagtaggaga aaacctagca ggaagagaaa tatcttatca
    25921 aggttcacta tcttcaagct gtataaaaat tatggatatg gaggcagcat caactggaat
    25981 tacagaaagg caagctaaag atttaggcat aaatgtcaaa tctaaattta tttctgatta
    26041 taatcaaact cattattatc caggaagaaa taaaatatat gtaaagctta tttatgatgc
    26101 agatacaaaa gttattttag gcggacaagt ggctgggttt aaggatgctg tccaaagagc
    26161 aaatgtattg gcagctgcta tctttggaaa aatgaccaca aatcaacttg gaatgttaga
    26221 tttatgttat gctccaccat ttgcaagaac atgggatgtt ttaaatgtag ctggaaatgt
    26281 atctaaataa tatttagatt atagctaata tagttgacaa gcaattaagg gaatgaattt
    26341 acctaaacta tttttatcat ggtaaaattc attcctaaat ttttattcca aatttttatt
    26401 ccaaattttt agaaataagg attccattaa attattcatc aaaatttaaa cgcaagctta
    26461 atctatcttc tccaagaata gtattttcaa aaacattagt agcattttct aaatacatac
    26521 taggtatgtc taaagaagga ctaaaatgag tcagtaaaag ttgtttgaca tttccaagtt
    26581 ttgcaagatt agcagcttct ctaaaagtca tatgtttgtt tctaacagct tttgatatat
    26641 ctaaatcatc accatacatg gcttcacata taaataaatc actatctttt atgaaatctg
    26701 gtattgattc tataggtctt gtatcggttg taagacttat cttaactccc tttctcaatt
    26761 ctcctaagac catatctgga gtgtattgtt ttgaatctaa aactatattt tgaccttctt
    26821 ggagtttgtt ccataatatt ttaggaactt cgtttttagt tgccttatct atatcaaatt
    26881 tagggtttct tctaaaatta aaactgtatc ctaaacactc tatagagtgt tctaaactga
    26941 ttgttgaaat ttctaaattt cttaaaactt tattatctaa agaaaaattg ccttgagggt
    27001 tttctataat ttttaaagtg tatggtacat attctactaa atttctcata gaacgtatac
    27061 aatcgactat tccaactgga ccaactatag ttaaatcact tgtacgacca ctattcccaa
    27121 ttgttgataa aagtcctaaa agtccaaata tatggtcacc atgtaaatgg gttatacaaa
    27181 tcaagtctat atctttaaag ccacattttt ttagcttcat agatacctga gtaccttcac
    27241 cacagtcaat tagtattttt ctacctttat agtttataaa tacagaggat agaaatctgt
    27301 ttggcatagg tacatttcca ccacatccaa gtaaagttaa atctatcatt ttttcccctt
    27361 tctaaaatta acacaaatct gttgtttgtt tttattatga tatcctatta taattatttt
    27421 aacattaaat ataacaataa atgtgtattt gagtcaagaa atattgtcaa ataaagtttt
    27481 tatatagaaa tgtataaaaa tttggaaaac aaaaagtgaa aattatattt aggagcattt
    27541 aaaggaggaa aatttaatga aagtattaaa gaaaattact attttgtcat tgattacatt
    27601 aaccttagtt ggttgtagta acggagaaga taaaaaagtg gttgaaggtt ctaagaaaat
    27661 tcaaaattct aaaaaagatg aacagcctat aatagtacat aaagatgcaa atatattgaa
    27721 agataggtat gagccaccaa aaggatttaa aagagttgat gtagaaaaag gtagttttga
    27781 atcatttctt aggaatacta agctaaaaaa ttatggagag aaagtaaagt attatgatgg
    27841 aagaactaag gaatcaaaag gtatttatga tagtgtattt gatgttgata ttggcaatag
    27901 agatttacat caatgtgcag atgcgattat gcttatgaga gcagaatact tgtatcagaa
    27961 taaactatat gatgaaataa gttttgattt tgttgatggt tttaaggcga agtattcaaa
    28021 atgggcacaa ggatatagaa tttcagtaaa agatagtggt tcaagctggt ataaagcaac
    28081 tgaatattca acatcttatg agagttttag aaagtttatg gatatagtgt ttgcatattc
    28141 aggaacgtta tctcttgaaa aagaattgga gcctgtaaaa gttgaggata tgaaaatagg
    28201 agatgtgttt atagttggtg gaagtccagg acatgcaagt ataattatgg atatggcaga
    28261 aaatgaaaag actggtgaaa aggtatttat gatagcccaa agctatatgc ctgcacaaca
    28321 aacgcaactt cttattaatc gtaaaaatcc aaatttgagt ccttggtata gtttagattt
    28381 tgaaggtgat ttgacaatac ctcaatggac ttttaaaaga gaccagttga aaagatttta
    28441 aaataataat tttatataat aaaagaaaaa tatagatgtg aaaaaatgta attagattaa
    28501 aactaattgg atatggacaa aattaaattg atagataaat attaaaa
//
LOCUS       DAEOSP010000029        28546 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.029, whole genome shotgun sequence.
ACCESSION   DAEOSP010000029 DAEOSP010000000
VERSION     DAEOSP010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28546
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.029"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(268..444)
                     /locus_tag="KRH54_001032"
     CDS             complement(268..444)
                     /locus_tag="KRH54_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578701.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(498..594)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1390..1791
                     /locus_tag="KRH54_001033"
     CDS             1390..1791
                     /locus_tag="KRH54_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBG8578702.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            1788..2855
                     /gene="rsgA"
                     /locus_tag="KRH54_001034"
     CDS             1788..2855
                     /gene="rsgA"
                     /locus_tag="KRH54_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG8578703.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYYDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LMGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKAEAVFADNKSE
                     YLAKKEKKFKNISKINKTIRR"
     gene            3391..4077
                     /locus_tag="KRH54_001035"
     CDS             3391..4077
                     /locus_tag="KRH54_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578704.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDSNKKEKVS
                     EDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIFDKTD
                     EQKKHINVLI"
     gene            4255..5145
                     /locus_tag="KRH54_001036"
     CDS             4255..5145
                     /locus_tag="KRH54_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578705.1"
                     /translation="MKKNILTTVITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGDNYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(5547..6503)
                     /locus_tag="KRH54_001037"
     CDS             complement(5547..6503)
                     /locus_tag="KRH54_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG8578706.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKKMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            7207..8202
                     /locus_tag="KRH54_001038"
     CDS             7207..8202
                     /locus_tag="KRH54_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8578707.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNYFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKTSVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            8180..9043
                     /locus_tag="KRH54_001039"
     CDS             8180..9043
                     /locus_tag="KRH54_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG8578708.1"
                     /translation="MSIMSNYNEASSNYSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            9040..9834
                     /locus_tag="KRH54_001040"
     CDS             9040..9834
                     /locus_tag="KRH54_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBG8578709.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWKQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLAMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            10119..11498
                     /locus_tag="KRH54_001041"
     CDS             10119..11498
                     /locus_tag="KRH54_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8578710.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVSQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            11946..12659
                     /locus_tag="KRH54_001042"
     CDS             11946..12659
                     /locus_tag="KRH54_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8578711.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(12931..14469)
                     /locus_tag="KRH54_001043"
     CDS             complement(12931..14469)
                     /locus_tag="KRH54_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG8578712.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVREL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            15351..15806
                     /locus_tag="KRH54_001044"
     CDS             15351..15806
                     /locus_tag="KRH54_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG8578713.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            15855..16439
                     /locus_tag="KRH54_001045"
     CDS             15855..16439
                     /locus_tag="KRH54_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBG8578714.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(16444..16767)
                     /locus_tag="KRH54_001046"
     CDS             complement(16444..16767)
                     /locus_tag="KRH54_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8578715.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            16919..17437
                     /locus_tag="KRH54_001047"
     CDS             16919..17437
                     /locus_tag="KRH54_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG8578716.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(17613..18281)
                     /locus_tag="KRH54_001048"
     CDS             complement(17613..18281)
                     /locus_tag="KRH54_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBG8578717.1"
                     /translation="MLEVSHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            18396..19811
                     /locus_tag="KRH54_001049"
     CDS             18396..19811
                     /locus_tag="KRH54_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG8578718.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            20034..20492
                     /locus_tag="KRH54_001050"
     CDS             20034..20492
                     /locus_tag="KRH54_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8578719.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            20489..21073
                     /gene="clpP"
                     /locus_tag="KRH54_001051"
     CDS             20489..21073
                     /gene="clpP"
                     /locus_tag="KRH54_001051"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBG8578720.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            21385..22461
                     /locus_tag="KRH54_001052"
     CDS             21385..22461
                     /locus_tag="KRH54_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBG8578721.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            22528..24594
                     /gene="bclA3"
                     /locus_tag="KRH54_001053"
     CDS             22528..24594
                     /gene="bclA3"
                     /locus_tag="KRH54_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HBG8578722.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGPTGA
                     TGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAVGATGIGITGPT
                     GPTGATGADGATGVTGPTGATGADGVTGPTGATGATGFGVTGPTGPTGATGIGITGAT
                     GLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGATGADGATGATGATGATGPTG
                     ADGLVGPTGATGATGADGLVGPTGPTGATGVGITGATGATGATGPTGADGLVGPTGAT
                     GATGADGVAGPTGATGATGNTGADGATGPTGATGPTGADGLVGPTGATGATGLAGATG
                     ATGPIGATGPTGADGATGATGATGPTGPTGADGLVGPTGATGATGATGATGPTGATGA
                     TGATGPTGASAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNA
                     AGTLTNFAFSMPRAGTITSISAYFSTTAALSLVGSTVTITATLYQSTAPNNSFTAVPG
                     ATVTLAPPLTGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAGYAS
                     AGIAIN"
     gene            24763..25524
                     /locus_tag="KRH54_001054"
     CDS             24763..25524
                     /locus_tag="KRH54_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG8578723.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            25833..26111
                     /locus_tag="KRH54_001055"
     CDS             25833..26111
                     /locus_tag="KRH54_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578724.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            26404..26979
                     /locus_tag="KRH54_001056"
     CDS             26404..26979
                     /locus_tag="KRH54_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBG8578725.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPNRLSTETLEQFHTNDDNLANQYAMNVELLHRNG
                     AEVGIPEYKIVGQEIDINDLTQVRIVSKYPSQLKVSALLREKLGLSRKAFDEMLDCGT
                     IKSISGVDLRRSKLNYEIVLEVGKQKQKSSN"
     gene            27418..28128
                     /locus_tag="KRH54_001057"
     CDS             27418..28128
                     /locus_tag="KRH54_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8578726.1"
                     /translation="MINIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSAGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            28122..>28546
                     /locus_tag="KRH54_001058"
     CDS             28122..>28546
                     /locus_tag="KRH54_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8578727.1"
                     /translation="MLTNLSVGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIIIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMV
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFL"
BASE COUNT        10681 a         3368 c         4766 g         9731 t
ORIGIN      
        1 aatttataat tttgttaatc taatccttca aactaaaaaa tcatttatgt ttacagaatt
       61 tcattttaag agatgtttat tttcacatag ataagagaat ttaagttctc atatttatta
      121 tttgatacaa atttacatct ttattatatg tattttaaat aaaaaataaa attatgaata
      181 ctagaaatct atcttaatta gctttctaac ataaggctat ttaaagtaga taaaactaaa
      241 aagggttctc tagaaaacca aatctattta attatactta cttactaata ccatcacttg
      301 atgcaaacaa gattctacca cattttctcc gtactctagt ataaaatcat ctagttgtgt
      361 atctataaaa tcacaataat catatgaact accaatataa gcagtcaaca tatgtgtaca
      421 tctatccatt ttatctctaa acatatatat tattatcccc ctaaaaaact ttcataaaaa
      481 caaaaagaat atctcttttt ggcaactggc tgacataact catagtttat tttataagcg
      541 ttagtcccta tagctttgcg tcactaaatt tctctagttt tgccgattta gttttattct
      601 acaattacaa taatacaata gaattgctta ttattcaata tacttatttg aaaattaaat
      661 aaatttacaa atatgaacgt tattttatat caaaaaaata gactatgcat gtagaatata
      721 tggaaaaact tgattgtagg ttttgtatga aaagacagtt gttattaatt taagtgagat
      781 aaattgagca ataggcataa aatgagaaaa caaataatag cagttaattt ttattattaa
      841 tactaggtga acattaaatt ttaaaatacc actagatttt atagaataag aaaatgagac
      901 aaacataaat atatgaatat attaaattat acttgaacat acttatattt tatgataaaa
      961 tttcaataat aattattagt gaaggagtgt cttgcctgat gtcagagtta tatataatgg
     1021 taaccaacaa ataaatagtt ggagacgcat atgggttctg tttaatcaga atttttaagt
     1081 gtatttttga tacatttgat tgttgtgtct tactctgcct ttatagataa ctaacattag
     1141 acaacaaata ataatattac atattacttt gttaaattgt ggtgtagtgg gatttaatct
     1201 gctacacttt tattaatttt acacagtatt attttgaata taattatgtt gaaatagaag
     1261 atattaaatt ttctagtcca caggggtttt ctgtttttgt gtaggtaaat atgtatacca
     1321 cataaaaagg cagatataaa tacctgtgga ctttttttgt agaaaataca agatacggag
     1381 gaaataaaaa tgaattataa aaatagaaaa cgagcttatt atcaggaaaa ttcctgtatg
     1441 gataacttta tttgcaaaaa ttgtggttgc cttgtagttc ctgaaggagc tggtacacaa
     1501 catagaaatc attgcccaca ttgtttacat agtcttcatg tagatatcat tcctggtgac
     1561 cgtgctgcag attgtgatgc taatatggaa cctattggag tgtgggtgag aaaaaatggt
     1621 gagtgggcaa ttattcatcg ttgtacacgc tgtggacatc ttagttctaa tcgtattgca
     1681 gctgatgata attcagtgaa attaatgtca attgcattaa aaccactggc acagccccct
     1741 ttcccactag aaagaatcat tgaaaagttg gaaaaggagg aaacagaatg aatggtatga
     1801 aacaaatcaa tttacaagac tatggtctaa ataggaattt tattactcaa agtatggaat
     1861 attatgattt ataccttgct cggattatct cgcaggaaaa gaacttttat caagctgtat
     1921 gtgtaaatgg tgtactgttt gcagaggttt caggaaagct gcgttttgag gctactatac
     1981 aatctgattt tcccacagtt ggtgatttcg ttatgttgga tcgttgcaat aataaaaatg
     2041 gtaatgctat tattcatcat gtgcttcata gaaaaagctt gtttgtgcgt aaggctgctg
     2101 gaaaggtaaa tgaggagcag gtagttgcag ctaatattga cattgttttt ttgtgcatgt
     2161 cactgaataa ggattttaac attcatagat tggagaggta tctgtcttta acatgggaaa
     2221 gtggagctat cccagtagta gtacttacta aatcagactt atgtgacaat gtgcatcgta
     2281 aattacaagt agttaattct atagccttgg gtgtagatgt agttgttacc tcatctatag
     2341 agccagatgg ttataaacag atttctaagt atattaaacc aggctgtact gttgccttac
     2401 ttggttcttc aggagttgga aaatcttcat taattaattg tctgatgggt ggagaatttt
     2461 taaatacaaa ggaaattcgc atagatgatg ataaaggaag acatgccacg actagacgag
     2521 agctattttt aatttcaggt ggtggtattg ttattgacac accaggtatg agagagttgg
     2581 gaatgtggga tgtttcagaa ggtttagata aaagttttgc agatatagaa gcttatttcg
     2641 gaaaatgtca ttttcgtaat tgtactcaca cctcagaacc aggctgtgct atctgggaag
     2701 ctattgctca tcaagaactt tcgcctaaaa gatgggagtc ctatcgtagg cttaaagctg
     2761 aagcagtatt tgcagacaat aaaagcgaat acttagcaaa aaaagaaaag aaatttaaaa
     2821 atatctcaaa aataaataag acgattagaa gataagtgta gtttttttgt tcattctatg
     2881 agtctatata ctattaatat attaaatttg ccatcaattt gttactttaa tgtgattttg
     2941 atggcaaatt tggtttgtgg tattatttaa agcaatttat taattttttt aataaagttt
     3001 gattttttat atgatgatat atacatgtga taatgtatac atatttgtta cgataggctc
     3061 ctttataaag aggtcttatg taagataaga aaaggcatat ttatactaat acataataga
     3121 gtatatgctt atgtatgata gagatgtttc ttaagagcaa aactgcaata ttgctaattt
     3181 ataaagtaaa aatatatgaa ttataaaata caaataatat atttataaaa attattgttt
     3241 taatttataa taaaaatttt ttgttgaaaa taatgctcga acctataaaa ctattaacta
     3301 atatttgtaa atgtatactt ttgatgataa aatataatta agttaagttg gtttttataa
     3361 acataaattt attttaagga ggtgaaataa atgaaaatat ctagtcaata tagaagtcaa
     3421 tattcattta gatatgaaag taatataaat aatacaagaa taaatgaaag tatggttaag
     3481 aaaaatgaaa ctgtaggaaa agacacttat ttatctaata ttatgaaaca aaagcaagaa
     3541 cttaatgata gaattagaga tttaaaatat agacaagagg tttatactaa aaaaataaat
     3601 gacgcaatta agaacttatg taaatcagaa ataagagaaa caactaataa cttttctaat
     3661 atagaaatag gtattaaaaa tagcattata gaagataaaa ataaaagtac aatgttagat
     3721 gaaaattcaa cttatctaaa tacaaatgat gaaaaagaat ctttaatcac taaagagtct
     3781 aatgaaaaaa ttgaagaaga aatattaaat gatgaaaaat tagaagagtt agaacaaaaa
     3841 aaggattcta ataaaaaaga gaaagtatct gaggacttat ctttagtagg taaaactcgt
     3901 gaagagcttg aaaatatgct taaaaatttt ataaatttaa cacaagaaga aataatgaaa
     3961 cttgagtcga gaatagaaaa gttagataaa aatgctgaag aatacaaaca aaattcaaag
     4021 actaatatat ttgataaaac agatgaacaa aaaaaacata taaatgtact gatttaaaca
     4081 atatgtttta aaaaaataag tttattatat aatgaaaata taaaggtaca atttcaatac
     4141 acttctatta aaccaataat ttatgaatca gacttaattt caatttacta ataaccatat
     4201 atttatgagt taataatttt atgttttaaa aagaattaaa taagggggag tagtatgaag
     4261 aagaatatat taactactgt gattaccatg attattatat tgatgatgag tatgacggta
     4321 atggcagagc catatcaaga agatgaaaca tctagcttga aaaaacagag tgcaaatgaa
     4381 cgtaatcaga ctaatatagg cacaatggat ggcagagtgc aaacaaatgc aataagtaaa
     4441 ggtgataatt atgaccctaa atatcctctt aaaggaatgt taacacaact tggtcttaat
     4501 acaacaggta aagagggatg ggatttactc tatggaggaa atcccaatgg agttgagctt
     4561 cgagcagatg gtaaatatta ttatataaat aagggtatgg aagggctagg attttatgcg
     4621 gcagcattgt caggtggctc atatcagaga ataggctttg gaaaacttga ccctatagaa
     4681 gtaatagctg atggaaattg gatttcttac aaacaagctc aagatacact tacagtaata
     4741 aggaattttt taaatagttt tgactggaga aatgcatctg agatggaaag agctaataga
     4801 gctgcaaaat tggtaaccga agcaaaatat gcagactcta aatattgtaa catagtctat
     4861 ggtaatctgg ttgataaacg tggtgtatgt ggaagttttg caagtagctt tcatttatta
     4921 acaaggttaa tgggaatgga ttcactatct atattaaatc catcattaaa tcatgcttgg
     4981 aactatatac aaattgatgg gaaatggtat aggtttgatg ggtctgaaat atcagctttt
     5041 ggtggatcat tagactttga ttaccgtaaa ttaaaggatg caacaagaga gatgactact
     5101 tactatgatg caaaggcatt gtctatatta ggatttaatc aataaaaata gggggaggct
     5161 tcttatagag ccttttttta ttgatttgaa atttaataag gaattttata tgaaaattta
     5221 atcaataaaa gcaaaaaagg ctctttataa agcctttcta agtgtttttt taagtaaaat
     5281 tttttatatc aacatcagtt ttaatcaaaa catataacat tttggactta cttttaaaaa
     5341 taaaattatt aaaatataat attaatcatg ttaataatgt ttttagttta ttatattcta
     5401 taatagtggt catgttgact attgtagttt tattaagaat agttggttta tataaatata
     5461 tgcaaagata gcttgttgtt tactttactt attagtatat aggacaagat actctaataa
     5521 atttgcctat tttaatatct tttgtactaa aaccaactaa agtttccttt attatttcgt
     5581 tttgaataat acaaagcata atccgattgt ttgtataaag actggaaagt atcagagtta
     5641 ttttctaatt tagatatacc tatagaacaa gaattactct ctaggcataa ttctaaaacc
     5701 ttctttgcct tattttcaac attctctttt gattgattac tatattcaaa aacagcaaat
     5761 tcatctccac caagtcttcc aataatagca tcatttacga atactttagt caaaatattt
     5821 gaaaaatgta ttagtatttc atctcctcga acatgaccaa agatatcatt tacctgttta
     5881 aaattatcta aatcaaagat gtaaaagtat ccatgtgaaa cagttttaag catttttttt
     5941 atgtgtactt caaaaaaatg tctattgtat agtccagtca taaggtcttt ttctgatttt
     6001 agtttaaatt catttgccat tgctagtgct tcttgtgctt tttcagttat tgttttaggg
     6061 taatactcat ctttcatttg gtcaaagttt ggaatagaat ggtgaacatg gataagttta
     6121 atcatatcat ctgtaactct gtataccata gtaaatcgag tatccatttc tacatagata
     6181 tctttttcta ggtaatcttt ttctattacc caaagagtac catatactat aaaagcttcg
     6241 tctgtgactt tggtacatga ataccactca tctataatct caaagtctat ttgctctgat
     6301 tctgtttggt ctttttcaag acctataata aattcttcta atgtttcgta aaattcatgt
     6361 tttcctgttc caattaatgc aaggttgtca tctaataagg aaataataaa tttaaggttt
     6421 tcttcttctg gatttaaaat ataaatatgc caaatttttt ttgataaatc tcttaattgt
     6481 ttttgtattg tattattttc catagattac ccccttaaga tttacagttt atattttaat
     6541 gtattataag ttgaactctt attacaatat tattataata tagaactaaa gaaattacat
     6601 cctgtatagg taaaagtata tcttttacaa taaaaatatt acagtaaaat gaccagtttt
     6661 aatatacgta ttatattttt aatttataaa cgaatattat aaatgaaatt taaattcaac
     6721 cttgaagaaa taagctaaat gtattagtat ataaatccat gcttgaacat aaataacctt
     6781 tatgatatga tatcaataat aatattatga gaggagtgcc ttgatgtcgg agttatataa
     6841 tggtaaccaa caattaaata gttggagaca catatattct gtataatcag aaatttcaag
     6901 tgtattttta atacatttaa tttttgtgtc ttattctgcc tttatagata actgacatat
     6961 gacaacaaat agtatttaaa atatatatta ctttgttaaa ttatggtgta gtaggcttta
     7021 atctgctaca cttttattaa ttttgtatag tattattttg ataattattt tgaaatagag
     7081 agcattaatt tttctagtcc acaggagttt tctgtttttg tgctgttaaa tataagcaca
     7141 gaaaaaggca gatatatacc tgtggacttt tttttatata aattatgaaa tatggaggta
     7201 gttaaaatga gtataattga acttaatgaa ctgactaaat cttatagaag atttgagaaa
     7261 aaggcaggat ttatggggag tgtaaagtct ttatttaata ggaaatatac agaaaaaaca
     7321 gctatatcgt catttgattt aaagatagaa gaaggagaat ttgttggatt aataggtcaa
     7381 aatggagctg gcaaaactac tcttattaag atgttaactg gtattattgc accgtcttct
     7441 ggagaaatat ctgtaatggg ttattaccct aacaaattag aaaataattt taaaaaacaa
     7501 tatgctgttg ttatgggcca aaaaagtcaa ctattctttg aattgacacc agcagatacg
     7561 tttcttttat ttaaagaatt atatgatata tctaatgaag aatataaaaa aaacttggat
     7621 tactttatcg aattatttga ggtatctgac tatttaaatg ttcaagttag gacgctttct
     7681 ttaggagaaa gaatgaaaat ggaactaata gtagcccttc tacataatcc aaaaatatta
     7741 tttttggatg aaccaactat aggcttagat gctatagccc aaaaacaaat taggtatttc
     7801 ctaaaagaag taaatgaaac aaaaggaact actattatac ttacttctca ttacatggaa
     7861 gatattaaga gtctctgtag tagaagtatt gttgtagaaa atagtcgtaa aatatatgat
     7921 ggttctacag agaaattatt taattatttt caaaaatata aaaaaataac agttagtttt
     7981 tccaatgaaa ctaacattgt atttccaaaa gattgtaaag ttgttgaaca aacaaatcat
     8041 aagacatcag taattgtgcc aaaagaaaga ggaaatgaaa cgattaaatg gatttttgat
     8101 aaatatgatg ttaaagatgt aatgattgaa gaagaagata taggtagtat tgtagagaga
     8161 atatataaga aaggaagtga tgagtattat gagcaactat aatgaagcaa gttcaaatta
     8221 ttcaatgttt tataaattta aacagagtat tagaaggtat tatgaagtag ccaaaattaa
     8281 ttttaaagtg caacttatct ggagatttga tgtggtggtt aatatggtgt ttacgattgt
     8341 aaaaatttta tttgcttata ttttatggaa agcaattttt ggtgaaaata aaatagtctc
     8401 tggttttaca tttaactcta tgttatctta ctatataata agctcatttt tagcaggtat
     8461 tgatatgtca aagagtacca gtgaagagat atgtaatcgt gtaaaattgg gcaccttttc
     8521 aaagtacatg attattccaa ttaatgttca aaattatttt attgctaaaa attttggaat
     8581 gacatgtttt tattttgtat ttaatatgat ttctgcattt gtatggattt ttatatttaa
     8641 aatagatttt gcctttacat caaatccagt tcaaatatta atttcaattt tgatgatttt
     8701 tctagggttt atttttatga tgaaactcca cttctttatt gggattttgt catttaaatt
     8761 tcttgagatt agttttttta gaatgataat tgaaaatatt gttgaattta ttacaggaac
     8821 aatgatacca cttgtactct taccagaaac tatagtaaca gttatgaagg tatttccatt
     8881 ttattataca acatatttgc catctatgtt actaataggt agaaacagca atgaggcaat
     8941 acaaggatta attattctag cattatgggt tttatttttt tctgtagtaa ataaagttat
     9001 gtataaaaga atgagaacat tatttgatgg ggtgggtata tgatgaaaaa aaatttgaaa
     9061 ataatattta tgttattaaa aatgaaatta tcaaagatga tggtatttag atttgatttt
     9121 tttggagcat tttttgtaga tagttcatta tttatattac aacttctaat gtttaatagt
     9181 atatattctc atgtagactc aataggggga tggaaacaag gagagatgtt aatatttata
     9241 ggaactttct cattaataaa tgcgataaat atgacgattt tcttttttgg tatttatgat
     9301 attccacgaa aaatccagga aggagaatta gactactata ttacaaaacc attagatcca
     9361 ctatttaggt taacatttga aaatattaat attggttcat caccattaat tctagcaagt
     9421 atacttattg ttttatatgg aatatctgag ttaaactcag agataacttt agctatgata
     9481 ataatatata ttttcatggt gttattgatg acattacttt tttatgatgt atgtatcata
     9541 cttagaacag tttcattttt tgttatatca tcttctgcga taatgagatt agaagataat
     9601 ttattgccaa tgaatatgaa aattcctggt gtaatatatg aaggtgtttt taaagtatta
     9661 ttctatttaa tcttaccata tggaattatg tcgacaattc cgactcaaat tttggctggg
     9721 acaataacaa ttaatggatt aatatactca attttaattg tatttttgtt tacattgttt
     9781 atgtggagat tttggaagtt aggtatgaga cattataaaa gtgcaagtag ttaataaatt
     9841 agtaaaaata gacaatgtat aactatatat aaatatgttt catatgacac taaactatgt
     9901 gaacaagtat ttgcgctaaa ggcactgaaa gctataaaat tattaaagta aggatttaaa
     9961 agtttcttta gtagaaatat aattgtgtaa aaaagcaaga ttaattaaaa ctatatccaa
    10021 ataattagat ataattctta tgcagaaagg aggtctgtaa atgtctaatt gtttggaata
    10081 tattttaaaa ataattaatg tacagaaaaa taaacaaaat gagtgatgta tattattcag
    10141 aaaatcatga acaagataat ctttatgagc aagataattt actggcacaa agatttaatt
    10201 tatttaccct attgaagttt gcatttccta ctatttttat gatgattttt atggggttgt
    10261 atacaatcgt agatactata tttgtatcaa gatttattaa tacaaatgct ctatcagcaa
    10321 ttaatattgt atgtccaatt attaatctta tagttggaat tggaactatg cttgccactg
    10381 gaggaagtgc tattgtagca cgtaagatgg gaaatggtga tattgaggga gcaaataaag
    10441 ctttttcttt aatcgtatta tcaggagttg taattggtgt aataattact ggtataggta
    10501 ttgtatttat ggattatatc attatatggt taggagcaag tgaagtttta attaaatatt
    10561 gtcgttcata tctttttatt atcttggttt ttgcttctgc aaatatacta caagtattat
    10621 ttcaatgttt atttgtaaca gctggaaagc ctcaatttgg attatggcta gtgttaggtg
    10681 ctgggtgtgc taatataatt cttgactatg tgttcattgt tctgttacaa atgggtatta
    10741 gtggagcagc acttgcaact ggagtaggat acctaattcc taccgtttca ggtatggttt
    10801 tcttttcaaa aaacaaaggg ttacttaagt tttgtatacc cagactagat aaaagaatat
    10861 tatttgaagc ttgttttaat ggttcatctg aaatggtcag tcaactatca acagcagtta
    10921 ctactttttt atttaatgct atgatgatga aattaattgg tgaagatggg gttgcagcta
    10981 ttacaataat tatttattct caatttcttt taacttcgct ttatataggt ttctcaatgg
    11041 gtgttgcacc aataataagt tataactatg gaagtgaaaa taatacgcag ttaaatcatg
    11101 tttttaagat ttgtatatta tttatttcag tagcatcttt attggtattt atcttttcta
    11161 tatcatgttc accaatcatt gtagagattt tttctcaaag aggaagccga gtttatgaaa
    11221 ttgcaaggtc tggattttat atattttctt ttagttttat tttttgtggg tataatattt
    11281 ttacttcgtc tatgtttaca gcattatcaa atggaaaggt gtctgcaata ttatcttttc
    11341 tacgtacatt tggatttata actataggtt tattgatatt gccaaatata ttaaatataa
    11401 atgggatatg gttagcagta ccaagtgctg agattttaac tttattttta accatttttt
    11461 gtatatgtaa gtataagaat agatataaat atatgtagat attaaataaa tagtttaggt
    11521 agatatttta tgttttaata agtaattcaa ttagtgggag atatatacta ttgaaaatta
    11581 aagttataat tgatataaag aattaataaa aataacatat aaaagtttaa aagggagttg
    11641 aacttaaagt ttgattagaa aaagtcaatt ttaagacaac tccttttatt ttactattgt
    11701 attaatctgt atctgtaagt gtattagtct aatattataa actttggatg aaattaattt
    11761 tctataggtt ttaatgtatc atacacttta ttttaaagaa acttatctta attttcatag
    11821 tgattgaatt tgtctattag agatgttact attagtaatg taaaagcaag agtagtgaat
    11881 ataaaaaaca agatagtgat actgcaagat tagaatataa taaattttta tggagagatt
    11941 tcaatatgga tacatgtttt aataaaaaaa tattacttgt tgatgatgaa aaggatatag
    12001 tagatttaat tgaagaagta ctcataaatg atggttttaa aaacattatt aaagcttata
    12061 atggtctaga tgctatttcg ctgtgtaaga tagcatgtcc agatgttgtc attcttgata
    12121 taatgttacc agatatagat ggtatagaag tgtgtaaaaa aattcgagag ttttcatact
    12181 gttctattct ttttttatca tcaaaaaatg atgatattga caaaatacta ggtcttagta
    12241 gtgggggaga tgactatata acaaagccat tcagtccaag agaaattgct tttcgagtta
    12301 aagcgcaatt acgtagacaa cagtatcaaa gcattgtacc atcagatagt gaagttatta
    12361 aaataggaga tattactatt gatatagaag gaaaccgtgt atataaagat agaaatgaga
    12421 ttgagttaac tggaagggag tatcatcttt taagctacat ggctcaaaat gtaaataaaa
    12481 ttataggaaa agaaagactc tatgaacaag tatggggagt atatagtagt atttgtgaca
    12541 atacaattat ggtgcatata cgacatataa gagaaaaaat tgaagataac ccctcaaatc
    12601 ctaaaatact tataacagtt aaaggactag gatataaact tgtgaataga atcgattaga
    12661 atagatttta atattaatga agatatctca agttgaattt ttagttaatg aggcattctt
    12721 ttatgggtga aatcaaatgt attttcgtta tctcaacaat gaagaagagg tctatctcta
    12781 ttttgagata gcctcattaa gtttatatcc tggtaatgta ctttatgttc taataaaatt
    12841 tttttagttt atttgcagtg aaacattaac tagaacatta aaatttgcat tgaaatacca
    12901 attaaataat attgtatatt gtgtataaac ttatactaat tgtttttcta tattaaatac
    12961 tttatcaatc caacctttat aaaatttttc ttcatgggaa actaggatta tacttcctct
    13021 aaactgtttc aaagcatttt gtaaagcatc ctttgtctct gcatcaaagt gattggttgg
    13081 ttcatccaga ataagaaaat tacaaggaga taacatcata caacataatt taaccttaga
    13141 ttgttcacca ccacttaaag tagatacact ccttgaaaca tgctcttcct tcacaccaca
    13201 tctggctaga tgacgtctaa tctcttttgt atttaatttt ggatatttat ctgctactat
    13261 ttgaagtggg gttttatcag gattttccca ttttaaatct tgctcataat aaccaatttt
    13321 aacttgttct gaaaactgga aatctccaga tatacgagga atatctttga ctaatgtctt
    13381 taacaaggta gatttaccga ttccattaaa accagttata actatttttt gacctccatc
    13441 aacagagaaa tttaattttg gaagtaaaga atagtagtat ccaacttcaa gatttgttat
    13501 agtaagagca ttttgagccg atatttcaat ttctgaaaat tgaatatttg gttttcctgt
    13561 aaaactagga ggagctattc tttctatttt atccaattgt ttttgtcttc ctcttgcaat
    13621 ttttgaattt actccagctt tatttttacg tataaatgtt tcttcttttt ctattttctt
    13681 ttgttgtgct tgataccttc taatataatc ttctcttaaa tgtgattttt gtttaaggaa
    13741 ctctgaatat tttccatgat atttcttaat catgccaaat tctatatcaa gaatacctgt
    13801 agaaattttt tctaaaaagt caaaatcgtg ggaaactaca ataaatgccc cattaaatgt
    13861 atttaaataa tttgagagcc attcaacatg ctctttatct aaaaagtttg ttggttcgtc
    13921 taataataga acatcatggt tagaaagtag taattttgct aaaattacct ttgccctttg
    13981 accaccacta agctctctaa caacacgatt cattcctatt gaatctaatc caagaccatt
    14041 tgctatttta tttatttcat tatctataga atagaaatta cttgctatta gttgttcctg
    14101 ataatctgat gctttgatta gatattgctc attttcagat atagcacttt tttgatagag
    14161 taaattcatt tccttttcta ttttataaag ttcttcaaat gcagtgtgta aatagagtga
    14221 tattgttgta tctctattga tttctgcata ttggtctaaa tggcctatat tgatattagg
    14281 ttgccacttg atttctcctg aatctggaac aacttctccc aataaaattt taattaatgt
    14341 acttttacct gtaccatttt ggccaactac acctatatgt tcacctttga ataaatcaaa
    14401 gttagctttc tcatacagtg atttatccat aaaacattgt gataaattag ttacttttaa
    14461 taaactcata attcaaactc cttttctttt attaaaaaat ttatggaaat aaaaaaagca
    14521 gaggaatggc attgtaaaat gccgacctct gcttttggaa aaacaaaagg tcatagacaa
    14581 gcattgttgt ctatgaccag aaaatctata ttatctatcg gcaaataggt aattttaaat
    14641 aagcatagcc aaaaaactat actaaaccta tatttgccga tgaagataga aagctttaag
    14701 ctttcagatt aacctctaca caacgcttca tcgtatacat ttgtgtagag ttaagttttc
    14761 taacaagttt aactaattct atataaatca taattattca catcctaatc ttagtattaa
    14821 ttctagttta tctgtattta aattgtactt ttgtactttg tggattatct tatttattta
    14881 aaatatgttt tattaattat agcttaagtt atgttatcat aatacttagt tatagtcaat
    14941 aagaaagtta tatttactat tattatatta atttagtaaa tctatcatta caaagttatt
    15001 tgaagactcg ctatcaataa aattttatag tctataagaa aattaagatt atgagtttta
    15061 taattttgat atagggatac tattttcata aatattacaa catgtagaag tatatgtcta
    15121 ttattgttga attttcaatg atatattaag gttctaatat attatacaaa ataaaaacat
    15181 taattcaact taaataattt taagtattat atgtgttttg attaaagaaa actaaaattt
    15241 taaggaaaaa taacatattt gtattaaatt ttattgaaat gattaaattt atttacttaa
    15301 tgtaaattat tagctaaaat tgtattaaat aattaaggag gtgagtagct ctggatgaaa
    15361 tagatttaaa aataattaat tctctaaaaa agaatagtag ggcttcaacc tctgaaataa
    15421 gcagacaaat taatttatca gtaccagcag tctcagagcg tataaggaaa attgagcagt
    15481 caaatttaat tcaaaagtac actattcaaa ttaatcggga aaaaacaaaa tataagcttt
    15541 tggcttttat atttatcaat actgatttat ctaaaagtat gaaagaattt aagaaaacta
    15601 tattggaata tgatactgta ttagaatgtc atcatatttc aggagagtat gattacttaa
    15661 taaaagtatt agcagaagat acaagtaatc ttgaagagtt tatatcagtg actttgaagg
    15721 aaattggggg gattcaaaaa gctaatacaa ccattgtttt atctacaatt aaagaagaat
    15781 ttaatacaat aaaagatatt ttttaaataa tttactagta aagtgttaat tatattttag
    15841 gaggaaaata gaatatgaaa gttttagttt ttttagcaaa aggttttgaa actatggaat
    15901 ttagtgtatt tgtagatgtt atgggatggg ctcgtaacga ttatgggcat gatattgatg
    15961 tagtgacttg tggatttaaa aaacaagtta tgagcacatt taatatacaa gtattagttg
    16021 ataagacgat agaagaagta tgtgttgatg attatgatgc tctagctatt cctggaggat
    16081 ttgaagaatt tggattttat gatgaagcat atgattcatc ttttttaaat ctaataaggg
    16141 agtttaattc aaaagaaaaa ataatcgctt ctatttgtgt ggcagcattg ccagttggaa
    16201 aaagtggtgt tttaaaaaat cgtaaagcta ctacatatca tttgaaaaat ggtaaaagac
    16261 aaagacagtt aagtgagttt gatgtaaatg tagttaatga accaattgtt gtagataaaa
    16321 atattataac ctcatattgt ccagaaacag ctccacatgt agcttttaag cttttagaaa
    16381 tgttgacatc taaagaacaa atggatgaag ttaaattagc aatgggattt aaactgtaat
    16441 ggtttataga ttttctaagt aatcagaacc ccagtcacac atggaatcta aaattggaat
    16501 aatactcttt ccaaaatcag atagcgagta ttctactttt ggaggaacaa caggataaac
    16561 cactcttata attaaaccat ctgattctaa ttcacgtaat tgttgagtta acatcttagg
    16621 cgttgcttta ggaattaatt tttttaattc attgaacctc aatatagtgt ctttaagatg
    16681 ccaaagtata agtgtcttat atttaccacc aatcaatgca agtgttgctt ctattgggca
    16741 actgtaatta tctaaacatt ttttcatttt tgggctcctt tatttatttt aaagtatctt
    16801 tttaggtact atattacaaa aaagtgcatt attgtaatat agtaccatag tgataaaatt
    16861 atagcatagg ttaaataaat catcaactta gcctaataaa acaatagggg gtaaataaat
    16921 gaatttcgtt gaacttgcaa aaaaaagata ttcttgccgt aattatcaag atagaaaggt
    16981 tgaaaaagaa aaacttgaga aggttttaga tgttgcaaga atagctccaa ctggtggtaa
    17041 tcgtcaacct caaagattga ttgttattca ggaaaaagaa ggtataaata aactttctaa
    17101 ggctgccaat atatatgatg cgccattagc aattcttgta tgtggagata aagacaaagt
    17161 ttggacaaga ccttttgatg ggaaacaact tacagatatt gacacaagta tagttacaga
    17221 ccatatgatg ttacaggcaa ctgaacttgg attggcaagt gtttgggtgt gctattttaa
    17281 tccagatata attagagaag agtttagttt gccagataat ctagaaccaa taaatatatt
    17341 attgatggga tatgaatcta agataccaga gagcccagag agacatgaaa aaactcgtgt
    17401 tcctttaagt gaaattgttt cttatgaaac tctttaatta taaaattaag ttattcatag
    17461 gataaatgta ataaagtgtt tgttataaac aaaattcaaa ataaataata tctacatata
    17521 attaatttta gtgaagtata tctagtttaa ctagaatgta tacttcacta atttatttat
    17581 tcaaaatatt tatgaggatt ttaatatatt aactaaaata tatcaattga attaactttt
    17641 aaatctggaa aagcacttga aatatagtgt tttatatcat ttaaatggtc ttgaatttgc
    17701 ttttctgata ttttatcagg tccatataca aaatacataa tatttttagt atcttttgtt
    17761 atagctgttc gattatcagg gccattaaga atactagata atataccaat attatctgat
    17821 aaaaataaat catcttttgt taaaatatgt tctttaccac ctataccata aaaatgttca
    17881 cttccatttg ctaattctat tttcaaaggt aattccattt tatcaaggtc atgacctgct
    17941 gttagaagaa gatttttcaa ttctgctaaa aacattgact caattgttac acctacatct
    18001 gggatacctt ttgattttaa tagaatagat tctaattgaa gcaatacagg gtaagttttc
    18061 ttgaattttt tataatactt tatataactt gaaacaggct ctgttttaat tttttctttg
    18121 cgattgtatt ctgggtgttg ttctataata tttttaattg tagagttttt aagaactaaa
    18181 aaattttctt tattaggttt tgaatgtgta atatttataa ccataactcc aaatttaata
    18241 ttgggataaa tttctttaac tttatgtgat acctctaaca taatatagac tcctttttat
    18301 tttatatttt atgatataat cttagcaaat taaaagtgga ttttatagtt ccatttttat
    18361 agtaatttta tagaaccact tttggaggta ttagaatgtg gattacgatt aatagaaata
    18421 gtaatatatc attatcaaga caaatttatt gtgagataaa agatatgatt ctggatggaa
    18481 gcttaaagtt tgagcaaaaa ctgccatcat ctaggacttt atcaaaagaa ctatctgtat
    18541 ctcgtaatac agttttagat gcatataatc aattaattgc agaaggatat ttggaaacaa
    18601 ggaatggata tggaacaatt gtagctaaag gaatatcagg gcataaagtt gattttaagt
    18661 taaatgatac ttttagagat gaaaaagatt tacatactaa taaacaaaaa aattatatag
    18721 attttcgttc aggagttcca gaattaaaac tatttccacg caaagaactt tcaaagctat
    18781 ataaacaaat actgtatgat ttatcagatt cggatttcag atatggaagt acagctggtg
    18841 tatgggtttt aagagtggaa atatctaagt atttatttcg tactagagga attagatgta
    18901 atcccaaaaa cattatgatt gtttctggtt caacacaagg tttatcttta atttcacagc
    18961 ttttatacaa aagtaataaa aaggttgctg tagaagaccc tattcataaa ggcttattaa
    19021 aggttatttc atctattgga tattcagttg ttggaattaa agtagatgat aagggaatga
    19081 ataccaactt aataaaatca ataaagggga tttcttttgt ttatactaca ccttctcatc
    19141 aatacccatt aggagggatt ttacctattc aaagaagact agagcttatt aaatgtgcaa
    19201 cagagaatga ttactatata attgaagatg attatgatag tgaatttaga tatgagggtc
    19261 aaccaattag ttctctttat gaacttagcc caaataaagt aatttacata ggttctttta
    19321 gtaaaatatt atctccagct attcgtgttg gatttatgtt attaccagac aatattttag
    19381 aaagctataa aaccttaaaa atgtactctg atgttcatac agaaacaata ttgcaacatg
    19441 tattagctga atttattgga aatggaggtt tagagaagta catatttaga atgaagaagt
    19501 tgtacaatag aaaacgtaag tacctcataa aagttttaaa cacggaattt aaaaatgaat
    19561 ttaaaataaa aggtgaagca gcagggcttc atatagttgc tcatttctat aatgttaaat
    19621 ttacggaaag tttaattcat aaaatttttg aagcaggtat aaaagtttat cctatacaac
    19681 aatatgcaat tgagaatata ttagaatatg actatgaaat tatactaggt tatgctcatc
    19741 ttagttttga tgaaattgaa aaaggtgtaa aaatactcag caaaataata catatgaatc
    19801 aaggtacata gataaaacat gtagcaatct acatataata gatgtaatta agtttaaact
    19861 ttaaaagaaa tatatattaa tatttttgta taaaaatacc tgatattttt ttatcgatac
    19921 tttattgcta tacactaaat taagatagca atttatatta agaaaaaact ttttgcatag
    19981 gttataatac ttaaaaggag aggatagagt tgaaaacaaa tacagatgtt attatggaga
    20041 taattgatta tatagaagaa aatcttacag aaaatctaaa tttagataaa atagcttata
    20101 ttgctaatta ttcaaaatac catttacaca gaatgtttac aaatgttgta ggatgtacta
    20161 tccatcaata catacagagg aggagactta ctgaagcagc tagacaactt atatttacag
    20221 acaaatatat tatagacata gctattacag taggatattc tacacatcaa tcttttacac
    20281 tagcatttag aaatttatat aaagaatctc cacaagtata tagaaataaa gatgaatttt
    20341 atcctattca acagagattt gttttaaatc taaatcatca aattcaaagt gaaaaatgta
    20401 tttcttatgc aaatatgaat tataaacaaa gaagagatga aatcaattct tatcttaact
    20461 ttaatattaa ttgtgaggtg aaagtagcat gagtttagtg ccacatgtaa ttgaacaaac
    20521 aggtcaaggg gaaaggtctt atgatatata ttcaaaatta ttaaaagata gaattatatt
    20581 tataggagaa gaaattaatg atgctattgc aagtttagta gtagctcaat tactgttttt
    20641 agaatcagaa gacccagatt cagatataat tatatatata aatagccctg gtggctctgc
    20701 tacatcagga tttgctatat atgatactat gaattatgtc agatgtgatg tatcgactat
    20761 ttgtattggt atggctgcaa gcatgagtgc ttttttactt gcaggaggaa cacatggaaa
    20821 aagatgtgct ttacctaatt cagagattat gattcatcag ccaatgggag gagctaaagg
    20881 tcaagcaaca gatgtaaaaa tagctgtaga taatatattg aaaataaaag aacgattaga
    20941 taaaattctt tctgaaaata caggtaaatc aatagaagat attaggagag atacagatag
    21001 agataatttt atgactgcac tagaggctaa agagtacggg ctgattgatt atataatgaa
    21061 taggaatgaa taaagtatca aagtatcata ttaaaatgtc tgttttacaa atatatatat
    21121 aatattaaat aagagtgtct caaaatgaac tttttagttt atgggacact cttttttgta
    21181 tgaaattaaa tatggtttta tcatttcaac aattattacc aaatttcaag tttaataaat
    21241 tctactataa aaaatacata aaagataagg ttctttagta actttctgtc gtcaaaagtt
    21301 tttataaatt taataacaaa acaagagaaa ataccatata ttacttatgt ctgcaatata
    21361 tagaagacaa aggagttgta cattatgatt acaataagtt tgtgcatgat tgttaagaat
    21421 gaggaagaag ttataggcag atgcttagaa tgtgtaaagg atatagtaga cgaaattata
    21481 attgtagata caggttctac agattccaca aaagaaatag tctcaaaata tacagacatg
    21541 gtgtacgatt tcgaatggat agatgatttc tctgctgcaa gaaatttttc attttctaaa
    21601 gcttctaaag actatactat gtggcttgat gcagatgaca taattttaga agctgataga
    21661 gaaaaattat taaagattaa agaaagcttg gatacttcca ttgatattgt tatggcaaaa
    21721 tacaatgtat cctttgatga aaatggtaac ccaactctct cgtattatag agaacgattg
    21781 tttaaaagaa gttctaatca taaatgggtt gaccctattc atgaagtagt acctcttttt
    21841 ggagaggtat tttattctga cattgcaata tctcataaaa agcttcatag acaggaccct
    21901 ctaagaaatc ttagaatttt tgaaaaaatg atttcagaag gaaaaacatt ggagccaaga
    21961 caccaattct attattctag agagttgtac tataatgcta ggtataaaga agcaatagaa
    22021 ggttttacta agtttttaga ctcatcaaga ggatggattg aggattgtat cagtgcttgc
    22081 agagatttag ccacatgtta ttacttaatt aatgatgaaa aaagtgcctt atattcattg
    22141 tttagaagtt tcgaatttga tgagccaaga gcagaaattt gttgtgacat aggaaagcat
    22201 atgtttgata gacaaaaata taaagaagcc attttttggt ataaggtagc attaacaaga
    22261 gataagaatg atactaatgg aggttttaag tcaaatgatt gttatggata tataccatct
    22321 atacaattaa gtgtctgtta tgacaggtta ggagaatcag ataaagctat ttattatcat
    22381 gaaaaaacaa aagaaatcaa acctaacgat agtgctgttt tgcacaatga aaattatttt
    22441 agtaaattta aaaatagttg ataggtcaca atcatactaa atttgacata ttatatatag
    22501 agcttaaaag ctcaaatata tcaggaggtg cttttaataa tgagtagaaa taaatatttt
    22561 ggaccatttg atgataatga ttacaacaat ggctatgata aatatgatga ttgtaacaat
    22621 ggtcgtgatg attataatag ctgtgattgc catcattgct gtccaccatc atgtgtaggt
    22681 ccaacaggcc caatgggtcc aagaggtaga accggcccaa caggtccaac gggtccaaca
    22741 ggtccaggag taggggcaac aggcccaaca ggaccaaccg gtccgactgg tccaacagga
    22801 aatacaggga atacaggagc aacaggatta agaggtccaa caggagcaac aggggcaaca
    22861 ggcccaacag gagctatagg gtttggagta acaggtccaa caggcccaac aggagcgaca
    22921 ggagcaacag gagcagatgg agtaacaggt ccaacaggtc caacgggagc aacaggagca
    22981 gatggaataa caggtccaac aggagcaaca ggggcaacag gatttggagt aacaggtcca
    23041 acaggcccaa caggagcaac aggagtagga gtaacaggag caacaggatt aataggtcca
    23101 acaggagcaa caggaacacc tggagcgaca ggcccaacag gagcagtagg agcaacagga
    23161 ataggaataa caggtccaac aggtccaaca ggagcaacag gagcagacgg agcaacagga
    23221 gtaacaggtc caacaggggc aacaggagca gatggagtaa caggtccaac aggggcaaca
    23281 ggagcaacag gatttggagt gacaggccca acaggtccaa caggagcaac aggaatagga
    23341 ataacaggag caacagggtt aataggtcca accggagcaa cgggagcaac aggagcaaca
    23401 ggcccaacag gagtaacagg ggcaacagga gcagcaggac taataggacc aaccggggca
    23461 acaggagcaa ccggagcaga tggagcaacc ggagcgacag gggcaacagg agcaacaggt
    23521 ccaacaggag cagatggatt agtaggtcca acaggagcaa caggagcaac aggagcagat
    23581 ggattagtag gcccaacagg tccaacaggg gcaacaggag taggaataac aggagcaaca
    23641 ggagcaacag gagcgacagg tccaacaggg gcagatggat tagtaggtcc aacaggagcg
    23701 acgggagcaa caggagcaga tggagtagca ggtccaacag gagcaacagg agcaacagga
    23761 aatacaggag cagatggagc aacaggtcca acaggggcaa caggtccaac aggagcagac
    23821 ggattagtag gtccaacagg agcaacagga gcaacaggat tagcaggagc aacaggagca
    23881 acaggcccaa taggagcaac aggtccaaca ggagcagatg gagcaacagg ggcaacagga
    23941 gcaacaggtc caacaggtcc aacaggggca gatggattag taggtccaac aggagcaacc
    24001 ggagcaaccg gagcgaccgg agcaacaggt ccaacaggag caacaggagc aacaggagca
    24061 acaggtccaa caggtgctag tgcaataata ccttttgcat caggtatacc actatcactt
    24121 acaactatag ctggaggatt agtaggtaca ccaggttttg ttggctttgg tagttcagct
    24181 ccaggattaa gtatagttgg tggagtaata gaccttacaa acgcagcagg aacattgact
    24241 aactttgcat tttcaatgcc aagagctgga acaataacat ctatttcagc atacttcagt
    24301 acaacagcag cactttcact tgttggctca acagttacaa ttacagcaac actttaccaa
    24361 tctactgcac caaataactc atttacagct gtaccaggag cgacagttac actagctcca
    24421 ccacttacag gaatattatc agttggttca atttctagtg gaattgtaac aggattaaat
    24481 atagcagcaa cagcagaaac tcgattctta ctagtattta ctgcaacagc ttcaggtctt
    24541 tcattagtta atactgtagc aggatatgca agtgcaggaa ttgcaataaa ttagattaga
    24601 taagaaaagg cttctaatat cgtattgata ttaggagctt ttttaagaat gattgatgaa
    24661 catagtttta atattaatta gtgcgcatat tataaaatac tattatataa tataaaataa
    24721 taacattaaa ttggaaatat agaattatct aggggtgata ctatgaaaaa taagattgaa
    24781 atatcaatga acaactttgt aaaaaattat cataaatcaa ataatataac tacaaagtgg
    24841 caagaacttt tgtttgctta tgcatctgct gaagatgata tgtttcagaa gctaaaaagt
    24901 gttgtaggaa catctcatgc tatgccaaaa gattttctgg aaaatggaaa aactataatt
    24961 acatatttta taccttttga tgaaagtgta aataatagta atattggagg gtttgaatgt
    25021 tctgaaatat gggcaagagc gtacatagaa actaatatgt tgatttcaaa tttaaatgat
    25081 ttcttatgtg aagaactaga gaagatgggg gctaattcat atgctattcc tgggtcgtat
    25141 aattttgaca gtgaaaaatt ggtaagtgac tggtcacaaa gacatgtagc attcatagca
    25201 ggtcttggaa cttttggttt aaataatatg cttattacag aaaaagggtg ttgtggaaga
    25261 ataggaagca taatcacaag tttagaatta gagccaacta aaagaccaga aaatgaatac
    25321 tgcttgtata aacataataa tagttgcaaa aaatgtgtca acaaatgcgt gaaagaagct
    25381 ttaaaagaag atggatttaa taggaataag tgttatgaaa tgtgtatgat aaatgaaaaa
    25441 aaatacactt atataggtgg atgtgatatt tgtggaaagt gtatagttgg agttccttgt
    25501 tcagtaataa acccatcaag ataatggatt taagttgact aataatttta aagatagttt
    25561 ataatgaaaa aattttagtt atcaaaataa atataatata aaaactaaat aaatgtgttt
    25621 aaaaaatgga taaataattt gtgttgattg acaaccagtt atactgttga tagacttata
    25681 tgtatcgctg tgatatataa ggagggttga gattttatgt ttgatttaat ctttattttt
    25741 gcatagcaag ggctattgca actatgaaat ttaatatata aatagccctt tgctattcct
    25801 aatgaaaaat tactatttaa taggagagca aaatgagcta taaaaaagca atacatatat
    25861 taccagaaga attacttgaa ttaatacagg aatatgtaga tggtgaatgt atctatattc
    25921 ctagaaaatc taataataaa aaagaatggg gaagtaatac acctactcgt gaggagttgt
    25981 caattagaaa tatgcagatt tacgaagact atcaagttgg atatgatttg caatatttat
    26041 cggaaaagta ttatttatct ttgaaaagta tccaaagaat tgtattacaa gaaaaaagaa
    26101 aagatatcta aggatgagcc agtatagttt tataattata ctggctcatt ttcttttgat
    26161 aaaagtgttt gaggtatagt ttcttttaag tggatgatat aatttaagta atttcaataa
    26221 cttatacagt agctaacaaa gctggttgta aagtatctaa taagaagtag tagcttttta
    26281 tatgtttgaa attaaaattt attaaaatta ggaggtcttg ttttgaaaaa aatacttgat
    26341 tatattccac cttttcataa ctttctatta atttagtaat cttatagaaa ggaaattgca
    26401 tttatgaaaa aaattacttg ggaggtggaa tatatctccc agttacctgt aattaggtat
    26461 tgtaaaaaat gtggaaaaaa gactgaatat atttgttcag gtttatttag ggttaatgct
    26521 caacataaat atttggatat atggttaata tacaagtgtt caaattgtga ttcaacatgg
    26581 aactcaacta tatattctcg tgttaatcca aatagattaa gtacagaaac actagaacaa
    26641 ttccatacaa atgatgataa tcttgctaat caatatgcta tgaatgtaga acttttacat
    26701 agaaatggag cagaggttgg tattccagaa tataaaatag taggacaaga aattgatatt
    26761 aatgacctta cacaagttcg tattgtaagt aagtatccat cccaattaaa ggtgtcagca
    26821 ttattacgtg aaaaactagg tttatctagg aaagcatttg atgaaatgtt agattgtggg
    26881 actattaaaa gtatatctgg agttgattta aggagaagta aattaaatta tgaaattgtg
    26941 ttagaggtag gcaagcagaa gcaaaagtct tcaaactgat tagaataact aacttaatat
    27001 attttcagga acatcattta aaaaaatagt gactttaaaa aatttaaagc cactattttt
    27061 taatatgttt tatatgagta tttaaaataa agtgtgtatt cttcaaattt atagcgataa
    27121 ttaaaacatt actataaaaa ggaaggtgca tactttttat ttaacaaaaa ctagatatta
    27181 aaaacttttc gaggaatatt ttattaaaat ccctaaatat atttaatata tattaaaaaa
    27241 ttgtattata atgaaaatca aaatagattg caaatccaaa aagtaatact taatgtccaa
    27301 aaagctaaaa ttaaaatcta aatagctatt tactacagaa gtatgttaaa atatgtaaaa
    27361 tgaatagaaa tttatatatt tttattaaaa atatataaga aaggatgttt aagaattgtg
    27421 attaatatag gaatatgtga tgatgaacta cattatcgaa taaaaataaa agatatatta
    27481 agtgaaatat taagttcata tcctataaat tataacatat atgaattttc agcaggagaa
    27541 gagttactaa ataactatcc taaagattta gacatattaa ttatggacat acaaatgaaa
    27601 acaataaatg gtatggatac tgctaggaag ataagagagt ttgatcataa gttagaaata
    27661 atatttgtta cttcatttgt agagtttatg caagaaggtt atgaggtaaa agcatacaga
    27721 tatatactaa agccaataaa taaagaaaaa atttctaaaa gtgtattacc ttgtataaat
    27781 gagatgatga aaaagagaaa taattactta actataaatg taaaaaatta tgtagaccga
    27841 attaaaatag attctattac ttatatagaa acagatagac cgaatatatt gatttataca
    27901 catgatgata tgtatattac taaaatgagt atatctaaaa tagaaaagat attaaatgaa
    27961 tatggatttt ttagatgtca taatagctat atagttaact taaaattagt agaatcaatg
    28021 agtggaagta ctgttatagt agatggaaaa agtattccaa taagtaaata tagggttaaa
    28081 ggcttgaagc ttgcgataac taatatatta ggggacatag tatgctaaca aatttaagtg
    28141 taggattttg gaatattatt aatttagttt caggtactat agattggatt atattttttt
    28201 tagtaatcca cttagttgga aagaggaaaa tatctaatat aagatatata tttacaacaa
    28261 taattataat tatatttatg gcatacataa atgtagttaa agtgtttcca aatattaaga
    28321 ttcttctatg tatggtaata ggaatatcat tttatataac atcatatgaa gataaaatct
    28381 ataaaagtat aatagttagt ttattgtttt ggctaggatt aatggttgct gaaggttctg
    28441 cagtaggtct agtagtattt ataaataaat taaataatat taacataaca caacatgaaa
    28501 acttatttag aatacaagct attattatgt caaaggtatt tttatt
//
LOCUS       DAEOSP010000030        27954 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.030, whole genome shotgun sequence.
ACCESSION   DAEOSP010000030 DAEOSP010000000
VERSION     DAEOSP010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27954
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.030"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(20..511)
                     /locus_tag="KRH54_001059"
     CDS             complement(20..511)
                     /locus_tag="KRH54_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578728.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(522..965)
                     /locus_tag="KRH54_001060"
     CDS             complement(522..965)
                     /locus_tag="KRH54_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578729.1"
                     /translation="MYSNTYNNENFMRARSFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(955..1794)
                     /locus_tag="KRH54_001061"
     CDS             complement(955..1794)
                     /locus_tag="KRH54_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578730.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(2013..3398)
                     /locus_tag="KRH54_001062"
     CDS             complement(2013..3398)
                     /locus_tag="KRH54_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8578731.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(3571..4314)
                     /locus_tag="KRH54_001063"
     CDS             complement(3571..4314)
                     /locus_tag="KRH54_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBG8578732.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(4334..5596)
                     /locus_tag="KRH54_001064"
     CDS             complement(4334..5596)
                     /locus_tag="KRH54_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG8578733.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARVMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(5780..6778)
                     /locus_tag="KRH54_001065"
     CDS             complement(5780..6778)
                     /locus_tag="KRH54_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBG8578734.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(6762..8273)
                     /locus_tag="KRH54_001066"
     CDS             complement(6762..8273)
                     /locus_tag="KRH54_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBG8578735.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(8327..9289)
                     /locus_tag="KRH54_001067"
     CDS             complement(8327..9289)
                     /locus_tag="KRH54_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBG8578736.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(9337..10227)
                     /gene="rbsK"
                     /locus_tag="KRH54_001068"
     CDS             complement(9337..10227)
                     /gene="rbsK"
                     /locus_tag="KRH54_001068"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBG8578737.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(10269..11282)
                     /locus_tag="KRH54_001069"
     CDS             complement(10269..11282)
                     /locus_tag="KRH54_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG8578738.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(11511..12488)
                     /gene="bioB"
                     /locus_tag="KRH54_001070"
     CDS             complement(11511..12488)
                     /gene="bioB"
                     /locus_tag="KRH54_001070"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBG8578739.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEF"
     gene            complement(12694..12957)
                     /locus_tag="KRH54_001071"
     CDS             complement(12694..12957)
                     /locus_tag="KRH54_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578740.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(13027..14100)
                     /locus_tag="KRH54_001072"
     CDS             complement(13027..14100)
                     /locus_tag="KRH54_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG8578741.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(14359..15117)
                     /locus_tag="KRH54_001073"
     CDS             complement(14359..15117)
                     /locus_tag="KRH54_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8578742.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTMYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISIVCLLAAVILFNKKPV"
     gene            complement(15114..16031)
                     /locus_tag="KRH54_001074"
     CDS             complement(15114..16031)
                     /locus_tag="KRH54_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8578743.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDGGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(16031..16231)
                     /locus_tag="KRH54_001075"
     CDS             complement(16031..16231)
                     /locus_tag="KRH54_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBG8578744.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(16236..17330)
                     /locus_tag="KRH54_001076"
     CDS             complement(16236..17330)
                     /locus_tag="KRH54_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8578745.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(17840..17980)
                     /locus_tag="KRH54_001077"
     CDS             complement(17840..17980)
                     /locus_tag="KRH54_001077"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBG8578746.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDIYPDSGWGFMTWDEN
                     GE"
     gene            complement(18290..19597)
                     /locus_tag="KRH54_001078"
     CDS             complement(18290..19597)
                     /locus_tag="KRH54_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG8578747.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVSGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(19662..20141)
                     /locus_tag="KRH54_001079"
     CDS             complement(19662..20141)
                     /locus_tag="KRH54_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBG8578748.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(20188..21051)
                     /locus_tag="KRH54_001080"
     CDS             complement(20188..21051)
                     /locus_tag="KRH54_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG8578749.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(21051..21806)
                     /locus_tag="KRH54_001081"
     CDS             complement(21051..21806)
                     /locus_tag="KRH54_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8578750.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(21833..22321)
                     /locus_tag="KRH54_001082"
     CDS             complement(21833..22321)
                     /locus_tag="KRH54_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8578751.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(22339..22740)
                     /locus_tag="KRH54_001083"
     CDS             complement(22339..22740)
                     /locus_tag="KRH54_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBG8578752.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(22743..23240)
                     /locus_tag="KRH54_001084"
     CDS             complement(22743..23240)
                     /locus_tag="KRH54_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8578753.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(23264..23668)
                     /locus_tag="KRH54_001085"
     CDS             complement(23264..23668)
                     /locus_tag="KRH54_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8578754.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(23898..26465)
                     /locus_tag="KRH54_001086"
     CDS             complement(23898..26465)
                     /locus_tag="KRH54_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8578755.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            26631..27374
                     /locus_tag="KRH54_001087"
     CDS             26631..27374
                     /locus_tag="KRH54_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG8578756.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHKWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNSNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
BASE COUNT         8871 a         5234 c         2830 g        11019 t
ORIGIN      
        1 taaatataaa aatcaactat tattttttaa atagcatctt tcttaatcta ttattgtaat
       61 aacctataaa agaactgtaa acatagtcat atactataaa ggctatctca aatgaaccta
      121 taataaatat gtttattgga atataaacta ttgttcttaa taaaaaatac aatataagta
      181 tcattatatt tgcaaatact aatttcataa aatactcaat atatatagac ctatcttttt
      241 ctataagata ttttacaata ccatatattc caaaggtaag tgaatataca atccattgag
      301 acttacttgc catcactata aatcctaaaa ctatagaacc tatataaaaa gcaattccac
      361 tcttaaagcc ccattccatt attacaattg atactaaaag tgatgctaac cccataataa
      421 acaatgtatt tacaggaata atgtttgtaa gagtcaacaa tatcacattt aatgctaata
      481 aaactcctcc ataagcaact tttatactca tatttatcat cctaacaaca agttattaaa
      541 tctccaccaa aacattcaca acatgaatct aaacaccaaa gtttagcaca attttcacaa
      601 caatcgtcac ctagacaaca acagcaatca tcacagcaac agcaacaaca tccatctaaa
      661 tcattacgtc ttcttctatt ataattgtca gccctattgc tataatcatt tctatattgt
      721 gtataacttc taagtgcatc actatattca ctattttcag gctccataaa ttttgctctt
      781 tttaagaagt cttcaccttc ttcataatat cctatgttca ttgcagaaaa accagttaaa
      841 taataccact ctgcacattt gtcagtcaaa gtttttaaaa aatcatatgc ttttttaaat
      901 tcactatttt ctatgaatga tcttgctctc ataaaatttt cattattata agtattactg
      961 tacattcttt ccacctctac tttctaatat attaatatat cttaaataaa caccagaata
     1021 aattatattt tctattattc caacattttt atttagattt agattatcaa ttcccctagc
     1081 taaaaatcct aatgatgtta ctattatttt ttttactttt tcttttaact catcattttt
     1141 atcaatatac tctataaatg gattatatct tcctttctta aaatcttcat ctaaatcttc
     1201 ataggcatct agtagatata tatacttacc tatattaaaa cctatcattc ttaagtttct
     1261 ctcattttca tcttttttat agacaaatac ttcacccatt atatttccaa atatactaga
     1321 tacttcatct atatttatat ttttatcttt ttctaactta tttaattcat ttagttgttg
     1381 ttttatatac tctgcttttt caggatactt ttcatatgct aattttaatt tgtttttata
     1441 tatataatat gccaatttat cttttatgcg tttatcatcc attaaattat cttccaattt
     1501 ataatacgtt aaaagaatat tcatacttgc tgcatattct gtaatatcat ttgtaattac
     1561 ctttttcttt ttaaatggat ttactataca cacttcctct gtacttatag actctgggtt
     1621 atatactgct gttaataaaa caattaaaaa tgttatatca tagttcaaag aaaacctaga
     1681 gatttctcca tgatttcttt ttaagtattt acatagtcca caatagtaag ctttgtaata
     1741 atcatattct ctaaatgtta aatccatttt gtttatttta acatatccaa acataaatac
     1801 tcctttttgt actatttttt atttagatta tatatagtat aacacttttt atcaagatta
     1861 tataatttac agtattctaa gaaacttaaa aaattccttc taatcattgt atcttaaatt
     1921 taaaataatt agaaggttgg gttaaaaata atattaaatt atatacttat attaactcat
     1981 acaagttagt tatgcttata tcaatttaag ttttaaataa ttggtatata tttaaaacta
     2041 cctacagtat ccttcaattc tttttttgct ttgtttataa ccaattcatc aagcattaca
     2101 tcatttattg aacatgatat agatttagat gccaataaca ttgctttaaa tcccatatca
     2161 gaacctgcac tggcacaaga ttgccatgta tgggcagaaa taccaatagg ccaaactgaa
     2221 caagaacact gtgctgtagg aattatgtag ctaacatctc caatatctaa tgataatgat
     2281 atacagttat tatgagtaaa tgtatcatcc gtaacttcat catgcagatt catcttagta
     2341 atatccttat ttccacttaa tacactagaa acactacttc ttttatctat agacactgtc
     2401 tcagctaatt tatctgcaaa atagtagtca tcttcattat agttttgcac acctactaat
     2461 ttcatatttt gtgatagaat atctgttaat attgtatttg gaatatagtc atatactcca
     2521 cttataatgt catattccat aatagtacca gtcattaatg ctgctccttt tgctatatca
     2581 acaatcctac caaatacatc tcttacatgc tccattttaa ctcctcttat gtaataccac
     2641 acttctgcaa aatcaggaac tatatttggc atttttccac cattagtagt tacatagtgt
     2701 actctacaat tatcaaatat atgttctctc aaataattac aacctatatt cataagttct
     2761 actgcatcaa gtgcacttct tccgttataa ggagcttgtg ctgcatgggc actaatccca
     2821 ttatatctaa atttcacaga gtaattagca agtgttggta ttcttattgg agtatttata
     2881 tcaaatggat gccaactaaa agcacaatca attccatcaa aaaaaccttt ttttatcatc
     2941 aatacttttc cagataaatc ttcttctgct ggacatccat agtattttat agtccctttc
     3001 agctttttta gtttgattaa ttcctttact gcaattacag ctcctattga acctgtacca
     3061 agtaagttat gtccacatcc atgacctgca ccaccatcta caataggttc tttagtagtg
     3121 gatacttttt gagacaaacc atctaaagca tcatattctc ccaaaactgc tataactggt
     3181 ctaccttctc cataactagc cacaaatgct gtatccatac cttctaatcc ctctatacta
     3241 aaaccatgtt tttttaaaca ttctttttgt aaatttgaag catagtattc tttatactga
     3301 agttctggat ttttccatat cttatctgat atatctctta attctccctg tatctcatct
     3361 atatattcca tcaaaaactt tttatttaaa ttatccatat taattatata aacctccctg
     3421 tttctaataa cttatcgatt atcagccaaa atgactaatt ttaactgatt taaaaatatt
     3481 ttctaccata caaccatttt atacaataaa acctcttgac ttgtaagttt cattttatca
     3541 agtaagaggt tttcaagttt tataaaattt ttagcatgtt aaccaatcaa gcatattaac
     3601 ccataattta ttatagtatt tccaatcagt aaattctttt ggaccccagt gaggtgcaca
     3661 gtcagaacta aagatagcag attttccttt tccaaactcg ccaacagcta caaatgggtc
     3721 tcctccaata gttgctaaaa caggacaatt atctctagct actgttttgt tatatcctaa
     3781 aaactttggc cattctgttg gtataccttt taatactgga tgttcagagt caataacttc
     3841 tggaattatt cctgctggtt tttcaactct atcatcttta tctatcattg ttattggtaa
     3901 tacatctttg atagcagttt ctccaaatct agcttttgca tctattcctg taaatgacat
     3961 atatccacct accatgatta acgccccacc attatttaca tactctttga ttagttcaag
     4021 tctatctgga ttactattgc aatctataaa agtactatta gataataaga acgtattaga
     4081 acctatatca gataatatta tacagtcata ctcatttaac tcttctaatt tatacggaaa
     4141 actatctgct gctacatgtg caggcatatg aactgtctca tatcctccag attctattgc
     4201 ttctttcaac cactttacag cttcttcata atgagttgta acaaacgtat caaacccttt
     4261 tatatgagtt atatttttta cccaagattc ccctactatt aatacttttt tcatttatat
     4321 attcctccta cttttataag tttaatatta gacttgcata cataagtata ctatctaaat
     4381 actccttaat ttcaacatat tcatcaattg aatgacattg ttctattgaa cctggcccta
     4441 atataagtgt tggtatttca gctaaatttt tcattactct cgcatcattg ccagctgttg
     4501 aaccttttat ctctaatttt tttcctagag tttcttctag tattttatgt gcattcacta
     4561 caaaatcaga atccttgtcc atctcaaaac ctaaccctga ttgatatatg tttatatttg
     4621 gtttattatc tttaagccat aaatctccat tagcccttgt cattatgaca tttgttacat
     4681 catttacaac ttgttcataa ctcattatat ttggcaagaa gtgtacacat aaattaaata
     4741 cacacttatc tggcacagta gaacctgctg ttccaccatt tattacacct aaatttattg
     4801 taggtgaagg taatagtgga tgtttatata tcataagcca gttatgttcc aactctttta
     4861 tatcttgaag caacaacata gccttctcaa ttgcatttac accctcccac ttactaccac
     4921 agtgaagaga cacaccttta acctcaactt caaagaatac aaatcccata tgtgcaacta
     4981 ttaagttttg ttctgatggt tcacaaatta tgcaacaatc tccatcaata ccattcataa
     5041 ctgcatttat ggtaccattt ccgccacctt cttcatctac tactgacatt atttttacat
     5101 taccaggtac atttagacca ctatctttta taagctttac agctaatatt gatgctataa
     5161 gtccagattt catatcagca gttccaagac cataaagttt tccattatct tctgtagcac
     5221 gatatggatt atatttccac ttacttatat cacccggtgg catagtgtct acatgaccat
     5281 taaatacaat tgtcttacca ggtaaatcat cactatactt acaaattaaa ttatatctat
     5341 cttgataatt gtgacctaga tttccttctt tatatatatt ttttgctttt ttaattaatt
     5401 cttcgctcat ttcttgtcta tccacactaa aacctatgta atttgcaagt ttttcaatat
     5461 attcctgtcc ttctttttca aatccaccta gtattccatg accaacatct tcagtcttta
     5521 tacttacaag ttcttttaaa taatctatgt attcttgttt tttactattt aatgtatcta
     5581 agagaatttt tttcatattt cctcctaaga tttattttta catttaaaat ttcaagtcta
     5641 atctcatcta tttaagccat agtaaacttt ttcacttagc atattctcat tcaatgtata
     5701 aagatgtatg caaattaaag tataaaaata tatacaaaaa cttaataatt tttatagttt
     5761 ataagctaaa atttaattac tatttagatt tagctgattt ttttaatttt atattattta
     5821 tccatatagc aaataacatt accaaacctt ttataatcat ttgattatat gaagatacac
     5881 ttaataagtt taatccattt cctaatattc ctattattcc gacacctata catgtaccta
     5941 ctatagaacc agaaccaccc atcatattag taccaccaag cacaacggct gctatagcat
     6001 ccaattctgc tccatctcca acagttggtg aacctgatgc caaacgtgat gatagtatta
     6061 tcccagctaa tgctgttagt agtccagcta gtgcgtatac ttttactata actttattta
     6121 cttttactcc acataatcta gcggcctctt catttccacc tactgcatgt acatgacgtc
     6181 caaaattagt tctttttaat agaaaatgtc ctgctattac aactataatc ataattataa
     6241 ctggtattgg tattggtcca atatatcctc taccgagcat cttaaatgca tctgtattta
     6301 ctgatatagg tcttcctcct gtaaatacaa agcttgcccc acttattatc gttaacatgg
     6361 ctaaagttac aatgaatgga ggtagttttt gtgttgaaat aattgcacct tttatgactc
     6421 ctaatattgt ccctattgct aatgcaagta tcatacctac ccatataggt gcattataat
     6481 tcactactgt tgtagcccaa aatgtaccta ctaaagcaat tactgctcct acacttaaat
     6541 ctattcctcc tgtaattatt acaaatgtca tacctactgc aagtattgca tttattgata
     6601 tttgtcttcc aacattaatt aaattatcta aagttaaaaa gttagatgaa gctacactca
     6661 ttattataaa aattattaca agtcctacag ctgcactccc ttggtctcta aatacacttg
     6721 ttaattttgt caataatgga gaagattttt cttctttatg cttagttatt gaactcatta
     6781 attttacctc cgaaagccaa ttttaataca ttattttcaa ccatttcttc accttctaaa
     6841 aatccactta tatgaccttc tctcataacc attattctgt cactcattga tattaattct
     6901 ggtatttcag aagatatcat tattatactt acaccactct ccactaaatc tcccattaga
     6961 ttgtatatct ctgccttagc atttacatct atacctctgg ttggttcatc cataatcaat
     7021 atatctggtt gtaataaaag ccatcttgct attatagctt tttgttggtt tccaccactt
     7081 aaactttgta taacttgatt catatgtggt gtttttatct ttaaagcttc tatgtatttt
     7141 tctaaaatct ctttttcctt ttttctatct ataattgatt taaacttaga tatctttttt
     7201 aacccagcca tagttgtatt ttctttgaca ctcatttgta aaaacaatcc agtacctttt
     7261 ctatcttctg ttattaatcc tagtccattt tctattgcat ctgatggagt tttaaacttt
     7321 acttcttcat tgtttaaata tatttttcca gaatcatatg ttctaagtcc aaatatagct
     7381 tctactattt cacttctctt acttccaact agtccaccta ttcccaatat ttcacctttt
     7441 cttatattaa agctaatatc attaaaaaca ttgttcttag ttagattttc cactcgcata
     7501 atttcttctt ttatttctac ttttcttttt gggaaaaatt ggtctagctt tctatctatc
     7561 attaaattaa ctaattcttc ctcagacgat acatctttgg tatttaaagt cgaaatcgtt
     7621 ttcccatctc gcattatagt tattctgtca caaatctcaa agagttcttc catcctatgg
     7681 gatatatata ttatactaac tccactctct tttaaatcat ttataagtct aaaaagtatt
     7741 tcagtctctt ttttacttat tgaagaagta ggttcatcca ttattaatac tttagtttct
     7801 gatgataaaa ctcttgctat ttctaccatt tgtttttgag ctatactaag ctctttgact
     7861 aaactatttg gatttatatc tattcccata ttatcgaaaa tctttttagc ctcttcattc
     7921 atttttttgt cattggttaa gactttgcca tttatttctt ttcccataaa aatattttgt
     7981 gctactgtta attcgtcaaa aactgttagt tcttgatata tggcatttat acctatcttt
     8041 ttagagatat ctggggatat attctctatt tctttgcctt caaaaataaa ttttccagaa
     8101 gttttcgaat gtgcaccagt taaaatcttt atcattgttg atttaccagc accattttca
     8161 cccagtagtg catgaacttc gccatgaaat aactcaaaat taacattatc taatgctctg
     8221 acaccaggaa attctttagc aatattactt aacttcagaa ttatattact cataatgaca
     8281 ctccttttta aataggattt ctttcaaata atctcatttt attttactaa ttcttaaagt
     8341 cttttatatt ttccttatca taaagtaaag ctggtactgg tatattcttt tctacctttt
     8401 ccccttttat agcttttact actgcttcta cagctaaacg tcccatctct ttaggttgtt
     8461 gttgaatcat agcaagaata gttccatctg ttattccact tgatgcttct tcagttaagt
     8521 caaatcctac aatcttaaca tccttatttt ttgcagattc aagtgcagct ttagctccta
     8581 gtacactatt ttcagctgtt gcatatatta tatctacatc tggattagat tgaattaagt
     8641 tttctactgt attaagagat tcttctctgt catatcctgg ttgtgaattt aatatcttaa
     8701 catttgctga accttttaat gcatctttaa atccatctat tctctgcatt tgtatttgag
     8761 attttaaatc tgtcactatt gctatttctg actttccacc taacttttca tctatgtatt
     8821 ttttagtgta ttcccctaat tttttaccag catcataact aactgttcct acataacttg
     8881 taatattttc agtatttaat acagcatcta ctgtaactac tggcattttt tcaaatgctc
     8941 cttctactgc tggtactata gccgaattat cagttccaag tactatcatt ccatctacat
     9001 tttgttgtat aaaatcttct attgctgatg tttgcttagc tacatcttgg tctgggtcat
     9061 ttactataac tttcacacct aattctttag ctttttcttt tattccctca tcaatatcta
     9121 taaaaaattg atgttgaaga gttattgtag atactccaat agttaaatct ttcttgtctg
     9181 aaccaccttt attatctgat ttacttggtc cttccattga acaccctgtt aacattccta
     9241 ttgctagtat tccagcaatt cctaaagata aaatttttct cactctcata attaacctcc
     9301 atttttaata gttttaaatt tgaacaaatt agatttttat ttattattta aatacttttc
     9361 aacatcttct ttatagggca taccagattg tgcacctagt ttagttacag aaagacctga
     9421 tactactact gcatattcta ctgctgtata taaatttttc ccttctgtta tagccactgc
     9481 caatgctcca ttaaatgtat ctcctgctcc tgtggtatct tgaacatcaa cacttataca
     9541 agatacatgc ttgatttcct caccatcata aaatctagct ccattttttc cttctgttat
     9601 tacaagttta tttggatatt tttttaatac ttcttctatc ccttcatttg tgtcaaaaac
     9661 tatcttatat tcatgttcat ttggtgttaa gtaagttact ttttctatta tatcttcatt
     9721 tagttttact gctggtgctg gatttaataa aaccctaatc ctattttcaa aacagaaatt
     9781 cactacataa tttattgttt ttaatggaat ctctaattgt agcaatacta tatctgcatt
     9841 ctttatctct tcttcattct ttttaattaa ttctatatct actagctcat tagtccctgg
     9901 tacaacaaca atactattat cattttcaca taatgttata acagcaacac cagaactttt
     9961 gtgattattt ctggatacta agctataatc aactttattc gttctaaaat ttcttattaa
    10021 ctcctctcct aagccatctt cccctatgca tgatatcatt tttacatttg ctcctagttt
    10081 agaagctgag atagcttgat tagccccttt tcctccaggt gatgtaaaaa aactattacc
    10141 taatactgtt tctccttttt ctggtctttt atctacagaa cataccatgt ccatgtttac
    10201 acttcctatg acaactatgt ttcccattac attactcctt tacatatcta tttatgtctc
    10261 tcgtgctgtt attcactttc ttttgttgaa tctcttatga ccaattctgg tttgaaaatc
    10321 aattcatcaa acgattcttt tttatctcct cttaaacaat ttatcaatat ttcactggct
    10381 ttatatccta tatcataaga cggctgtttt actgtggtaa gagaagggct tattagactt
    10441 gatgtttgaa tatcatcaaa tcccacgaca cttatatcat ttggtatact taatcctctt
    10501 tctttaagac ctcttatgac acctattgca atcaaatcat ttgcacaaaa tattgcatca
    10561 aaatctatct tttctaaatt ttttataaat tcttgtcccc attcaatctt atattttcct
    10621 tcaaatacat aagcaggatt gtagcttaaa ttgcactcga ttagagcctt tttatatcct
    10681 tctaatctat ctactgctat ttcattttga agtggaccac ttaggtatat tattttctta
    10741 tttccaatat ctaataaatg ttttgtagct atataggccc cttcaaagtt atttatttta
    10801 acaattcctt ttaaggtttc tttgttcata cttatcttct tatcaatcaa gacagttggt
    10861 atttttatat ctttataatt tgcttctttc cagtttgtat ttgaagatgc tgcaaatata
    10921 attccatcta tcatcctctc tgatagtgtt cttattcctt catattcttt tttagcatct
    10981 tcatctgtat tacataaaat tatattgtag ccttcttcat tagctttatc ttctacacct
    11041 cttgatacat ctgtaaagaa tggatttcgt atatctggta taagaagccc tatagtatta
    11101 gtttttttag ttactaaact ttgtgctaat ttatttggta cataattttt ttccttcatt
    11161 attttcaaaa ttttttctct tgttgcctga cttatatttt catctttatt attaatgact
    11221 tttgacacag ttgttttaga aacaccagcc aattcgccaa tttcttttat agttatactc
    11281 aagaagttca cccccaaaat ttaggaaacc gttttactta accggttacc tagatactat
    11341 cacatctttt tttcatttgc aaccatttta tataaatttt gatatttttc ccttttttca
    11401 aaatattgca acttataatt caattttaat atttaaatta atctgcaaaa aaaaattaat
    11461 atacttttat aattatctaa aagtatatta attaatttaa gcagtattta ttaaaattca
    11521 aatccttgct ctataatcat ttttttatca ttttcaatcc cacttccaac agttgtcaaa
    11581 taatcaccaa caattgcaga atttactccc ccattaaatc caactttgtc atatcccttt
    11641 aatgatagcc ttccaccagc ataccttatg tacacattag gaattataaa cctaaatact
    11701 gctgttgttt ttagtatttc cattggttct aatacatcat aattttccat tggtgtattc
    11761 ttaataggat ttaaaatgtt tattggaaat gattttacat ttaattctcg tatttcaaaa
    11821 gccattttta gtctctgttc ttcactctca ttcattccaa ttataccgcc tacacataca
    11881 tctagcccag cttttttagc atcttttata gtttttatcc tatctttata tgtgtgtgtt
    11941 gtacatatat tatgaaaatt atctctacac gtctcaacat tgtggtgata tgtagtcaca
    12001 cctgctgact tcaacatttt agcttgctca taatctataa tacctaaaga tgcacataat
    12061 tttaaattag tattttccct caatccttca tagatattta aaatattatt aaactccttt
    12121 cctgacatac ctttaccact tgtaaccaat gagaatctat gcactccttt actctccatc
    12181 tctttagctc tatttaagat ttcattgtaa tctaataatg aatattcttc tacaccagtt
    12241 ttataataag aagactgagc acaaaattta cagtcttcac tacatttgcc agatttagca
    12301 ttcattatcg tacataaatc tgcttttctt cccataaaat attctcttat ttcatttgca
    12361 gattttaata gaatatcaaa atcgttttta ttatttgtat caaggcttat taaattaaga
    12421 gcttcttcat aacttatttc tttttctctc aatactttat tttttaattt tattatgtat
    12481 ttttccatat atcctcctat ttgtcaactt aatattttta tgtagtttac ttattatata
    12541 tagtattgca tatataacat ttattggtca actttatttt agacacaagt ttactataca
    12601 caaaaaaata ctgcttcaaa acaatattag tatatagatt atacatatta ttattaaagc
    12661 agtatttttt attagaatat aattcttact ggtttataaa actatccttg tcttaactcc
    12721 atccaagctt tccattgtaa taacttctaa gccttcatat tctctaaata gccattttaa
    12781 atccttcatg gcttgattaa cctgactttt tgttagatta tttataaaat attcttttct
    12841 agtttcctca tactctttgc tagtcaaaaa gtttcttaga aaattaaacg caaatacact
    12901 tggaatattg tttacattag ccttcttgct gttttccttc tcatagtaaa ttttcataaa
    12961 tgcatactcc tttgatactt ttagatttct cttacaataa ttatatgcat ttaagtgcta
    13021 ctattcttat aattttatta attcatattc tttacttccc atacccattt cacatgcata
    13081 gtctattatt ctagttccat ttacatgagg atgttcatgt ttgaaaacat catgacccat
    13141 ttccttacaa atcaaatcat agcatgcttg ttcaatagct acagggtctg ttgatgctaa
    13201 tattcctata tcatgggcaa ttggtctacc tgaccaaggt acacaatcac aaagtggagt
    13261 tacattcata acaaaagtta tataccctac cttgtctttt ttatttgata ctgctccata
    13321 agcgtattct gccatctttt cgacaaacac atctgagtca ctttcccatt gtatagttac
    13381 tgctctagtt ggacagacag ttggacattc tccacatcca taacatacat cagaatctat
    13441 aactgctttt ttatccacta tacttatagc ttttgtagga caagaattaa cacactttcc
    13501 acaacctaca cacttctttt cctttactac tggagtaaca tcagaatgtt gcatttgttt
    13561 tcctgctgca cttgcacatc ccatagctaa gttttttaat gctcctccaa aacctgctgc
    13621 ttcatgacct ttaaaatgac tcattactat cattgcagaa gaattataaa tttctccacc
    13681 tatttttaca ctttcaaaat gttttttgtc tatttttaca ttctcataat ttctactata
    13741 taatccatct gcaattatta ctggtgcatt tacaactgca taagcaaatc cattttctat
    13801 agcagttgta agatggtcta cagaatttgt tctactccct gtatacagtg tatttgtatc
    13861 tgttaaaaat ggctttcctt cgcagtcttt aaccttatca accacttgtc taactgccac
    13921 aggactcata tatgtagtat ttcccttttc tccaaagtga agtttaattg cgacttggtc
    13981 atttttttct attaaatctt tgaatcctgc ttcatcaaat aatcttctta cattgtttgg
    14041 tacgttttta ttttgcgaat ttgagtataa atcacaaaaa tatacttttg acttttccat
    14101 atctacaccc ctttaaaata tttacttata ctaattttat cacttttaaa gtaattttta
    14161 gtatatttta acataattaa atattcttat atttatttga tacaaataat attatttgta
    14221 ttattttcta cacttaattc tatatattaa actacaccta aaaatcaaaa agacttgaaa
    14281 cttaagtcca agtctttttg atttttaggt gtagttttta acaacataag attactttac
    14341 cttgtgataa aggtattatt aaactggttt tttattaaat aaaataacag ctgctagtaa
    14401 acatactata gaaataagaa ctgttataat aattggaata ataaattctg gcattgccac
    14461 tttccctgaa atcaagtcta tattttttga catcaagaaa ttaggagtat aaggtgcaat
    14521 ctgtttagga atagaaatta aactcaataa gagagttgct accaatacaa gtaaaacact
    14581 ggcaaatgct ggtctaaata gtacacaccc aagaattaat acactaatat acataattcc
    14641 tataagccat aaattaaatg ctgaaaatat aatatgatta agatttgcag ttggccaaaa
    14701 atacattgta tagccataag caaataaaaa actcaaccaa aaaccaattg tcataataat
    14761 caccgtaaca gagaatttag acaatactat agatgaacgt gataaacctt tagtcagcat
    14821 aataacaagt gtccctttag aatattcatt tgataaaata ttacaaaaaa taattagagt
    14881 taaactcata ccaagaccag agatattgct aaaaaattgt gtccaagaat ctaaagcagt
    14941 tggtgcagca gtaactttta aagtcggtgc aaaatgagca atcaagtctg gcataaattt
    15001 tgcagaaata ggactactta taccaaatat tagaaataaa ataattaaac taaataattt
    15061 ataatttctt aaattctctt taaattcttt ttttgtaaat gctatgtatt ctctcacttt
    15121 actacctcca aaaataaatt ttcaagtgat aattctatta gttccatctt tactggacaa
    15181 agattcattt ctgcgagtgt agatataact gttttttgta ttgcttttat atctttacca
    15241 tgcaaaacaa tttccatatt agtttcttct gaatctttta gtaaggattt aataccatca
    15301 ctagacttaa acttttgaat ttcatcatta cttgcaaact ccagtaaaag tctatccttt
    15361 ccgtgcatac tttttatttc tgaaagtgta ccactaaggg caatgcttcc tttgtttaat
    15421 atagcaacat ggtcacagat tctttcaaca tctgatagta tatgtgttga aaatattaca
    15481 gttgttgagt ctttgatttt aagcataata tctaatattt cttttcgacc aacagggtca
    15541 agtgcacttg ttggttcatc acaaataagt aatttggggc gtgaaagcaa agcttgagct
    15601 ataccaagtc gttgcttcat tccacgagaa aacccaccta ttcgtctctt ttcatttcca
    15661 agacccacaa gtgataacaa ctcttcactc cttatctgta tttctttttt tgaaagtccc
    15721 gtaatttcac cacaaagaga aagatattca agtggtctca tatagttgta aaactctgga
    15781 acatcaggca gataacctac atgacgatta gaacttgttt tcccataggt cactttctca
    15841 ccacaaacat ctatagttcc accatctggt ttaagtaacc caagtaccat tttcattgtt
    15901 gtagtcttac ctgcaccatt cttacctaca aacccaaaga ctgacccttc tggtacagta
    15961 aaatttaaat tatctattat ctttcgatta ccaaaacctt ttgataatcc atttattgag
    16021 acaatattca tcattcttca cctcttccta ttgtaaaata caaaattggt cctattagac
    16081 taataaacag tactacaata atccatattg ccttatttcc aattttataa tttggatgtc
    16141 tcaatacatg aacaagtgca gtaattataa gtattaaatc taaaataata acaggaacca
    16201 aaagtggtaa gtattccatc aaattttcca tactcttatt cctccaataa agataatttt
    16261 tctataagaa atttgtattc tttgttttca cttaatacag aaaacattgg attattttta
    16321 atagcagcaa caatacctgc ttttacgatt gtatcatcac gtgtaatacc tgttccaatt
    16381 tcagaaatcc atctgtcaag tttattaaaa aagtcatccc catgtattat aattgggtat
    16441 atatcacgtg ttgctaaatc tacatatttt cgtaatgatt taatagctcc ttcattatca
    16501 ccatgtatta aattaatttg tgcctgtgtt aaatatgaag aaagcataat agcaggagat
    16561 aatgtatcaa gctcaaatat atctgaaatt gctagaatac gtctttctat ttccttcatt
    16621 tgtaatttat ctgcattttg caaaataccc actagatttt ggactaatga tataaggttc
    16681 tgatatgcac caagttgaaa tgtttctctt gcttcatcca tttgaccatt catcatttgc
    16741 ccttgtgcaa gaagtataga ttcatttatc atgggaaaat tgctgttttg caataaatca
    16801 atcacttgta tgggttgatt taacaatata taacaagttg cctccatact ttttgcttga
    16861 cggcaaatat ctatgtcatt acaagtttcc tgtatacgaa taaatatttc taatgcttca
    16921 ctaattaata gttcacgctt tttttcatct acaatatcat agtgattaac tattaaaaga
    16981 cccatccgaa aaatcaaagg aaaacatgaa taatattttt ttatgatttc tcttatttca
    17041 tccatgactt catcaaatgg tttaaccgta aagtctttgc acagtttatt atacagttta
    17101 ttaatatctt ccttcatcat ttgaggttca taacatatta actcatcaac ggtaatgttg
    17161 aaatatgtcg cgagttgcgg taaaaaggtt atgtcaggat agctttgtcc agtttcccat
    17221 ttagatacag aagctttaga aacgcctata taatttgcaa gttcatcttg tgtaattcct
    17281 ttttctttac gctttaaaat cagcgttttt gcgatgttta attcattcat gtcaatccct
    17341 ccttatgtat aattatatcc ttaaacatgt aaagctaaca agcgatttga agttgacttt
    17401 tgacgaaaaa atcaaccaca gagaaacaaa taaacaagta tataaaatat atatttaaat
    17461 ttatctttaa tctcctttgt aaaatatgta aactttgtaa ataaaatcca tattatataa
    17521 aactaatagt taatctatct aaatacagtt tttatttatc acgtttttaa atattttgtg
    17581 taaaacctct tttaatataa acaccttatt ttcatttctc atttcatatt ctttttaaaa
    17641 tactgaatat aactcttcga ttttccattt aatcctttta taaagttatt attcgtattt
    17701 attcctctaa ttataacagg caactatcat gtattggtat ttgtctatta tataaaaatc
    17761 tataaaagaa aagcagtcaa tcatttagat taactgcttg tacatatatc ttattaaact
    17821 gtataaacta taatttttat tattctccat tttcatccca agtcataaat ccccatccac
    17881 tatctggata aatatcgata gacttttctc taatcatata accaacttta taacactctt
    17941 ttgttatttt attagatagt atacatcctt ctccatttat ctaattccat ttaattaact
    18001 tttcttttca accttaactt ttataaaatc atctttttta ataatttcat acttattttc
    18061 tcttcaacat catcaaatga gggtgtctca aaattaactt tttacttcat aaggcactct
    18121 tttttgtatg aagtaaaata tattttcatc atttcaacaa taagtaagag gcttatctct
    18181 attttgagac agccccattt gatggtgttg aacatttaaa taaggttgaa ctcgttttat
    18241 ctctcttttt aagatatctg taaatttgca cataaagtct cttcttcttt tacaattcta
    18301 atagttttat taaagccaaa gtatatatct ccatattctt tcgtatatga tttattggta
    18361 ctctttcatc tactccatga ccaccagttt tatacgctaa gaacttaggt ccaaacgcca
    18421 ccatatcttt aaaccaactt gcatatgtgc caccacccat agcaattgta tatccatttt
    18481 catctgttac ttcttcatag gcttctaata attttttaag tactaaatta ctctcatcca
    18541 cataatgtaa gtctttatga gcaataacct ctattttcca accattacaa aaaacctttt
    18601 tgaatctatc tattatattg tttaaaacta tatttttacc ataccttata tctaactcac
    18661 atacgatttt ttcctcattt acttttaata ttcctaaatt taaggtagtg tttttgaata
    18721 cctcatcata ttgattaatc cccaatgcat ctccataata atcactacaa aggtattttg
    18781 aaactaattc tatatcttct ctatgctgaa aatccatatt atcctcaact atctttatta
    18841 ccattttaga tatagcattt tctccctttt caggattact tgcatgtaca gctactcctt
    18901 tagtagttaa ctctgaaata attccattaa taatttttgc agtacaatga gatggaacaa
    18961 cattttcttt agttcctcca cttactatta tgtaaggttt acttttgtca attttaattt
    19021 ctttttctat ccttatatga acccttcctt tttctccatt aaccactgga tacatagcat
    19081 ctggagtaaa tcccatactt ggaatttctt cacctttatc taagtaatat ttgatacatt
    19141 tcatacctgt ctcctcattt gtaccaaaaa ttaatcttat tcttttatta attgtaggat
    19201 acatatcctc tatgtttttc aatacatgta taagcataga tacagcaccc ttattatcta
    19261 tgacacctct tccataaata atattgtcaa ttactgctcc cgaaaatggg tctacactcc
    19321 acccctctcc tggtggaact atatctaaat gcattggaat ccctattaat tcctctccct
    19381 ctccaatttc tgcatatcca atataattat caatattttt aacactaaaa cctaaactag
    19441 cacagtaatc taaagtttta tctaaagatt ttttaggtcc tataccaaaa ggacattgtg
    19501 aaataggttc accttgttgt gaatcaattt ttatcaattc ttttagaaat tctatggttc
    19561 tatttaaact attatctaca cttctgctta tatccataaa tcaacctact ttctatcttt
    19621 ttaaagcctg tccttgaaga acaggctttt agcattaaaa attatttttt tgtaaatact
    19681 aattcacaaa cttcatcatc atgagcacat ttttgagcaa aatctaattt aacaccttgt
    19741 gcacagtcaa ctcttccata atccccataa caagctaatc tacaaatttc ttttctttct
    19801 tctttagtta atccatattc tttccaagca gcatctaatg gacagtgatg gaattttaaa
    19861 acgccattct catctgtatt ttctactaat tccatttcaa acactttttg tcctttactg
    19921 ttatataaca tttctgccat ttttcctgga gtatctgcta cgttatattt ttgacctctc
    19981 attttaccaa atccatatat tgcttcttta catatatcat ctggattagc tcctaatttt
    20041 tcagcttctt tcataagaag atagaaccat actgctcttt ctcctgttgc tcctctaatt
    20101 ccttctaaat attccttttc tgtatacatt ttttctgaca tttctttctc ctcctaaaat
    20161 taaattctat attttaatca ctgtgaatta gaatattcca atgaatgacc ctaccaatgc
    20221 taatacaact aaaattacta ataacttagt tattgtccac ttcttgtatt tcattcccca
    20281 atatacgaag aatacaacac caagtggtaa taaacctgga gcaatcatat ttaatgtatc
    20341 ttgaacaaca attgctttac caccaatatc aaatgatagc ggagttgaaa gcttaactgt
    20401 agttgcagat aatgctccca tcataaacat tcctagaatt gaagcaccat caattatctt
    20461 attgacaata ccacctgata gaattttctt aattgaatct ctacctaaag aatatcctaa
    20521 atgaaataag taataacctt cgcaaaatag taatatacta aacattattg ggaaaaatgc
    20581 acctataaca cttccttctg ctccaaatga agcacctaaa gctaagaata ttgtttgaaa
    20641 tgtaccaaaa tctaaagtat ctccaattcc agccaaaggt cccataagac cattcttaac
    20701 acctgaaata acttctcctg ggatttttcc ttcagtagct ttctcttctt ccatagctaa
    20761 aactgtaccg tgaattaatc ctccccatat agcttgagta ttaaaaaatt gtaagtgtct
    20821 ctttaacgca tctactaaat cttcttttct cttatataac ttttctaaag ccggagtgaa
    20881 acttgcacaa actgctaatg cttgcattct ttcaaaagag ttggataatt ctgctgtcca
    20941 ccaccatctt agatatgttt ttgttacatc tttctttgta agtactcttg tcttattaac
    21001 ttcagttgga ccttgtccat ttttaacttc tttatttttt atttcttgca ttatacttca
    21061 cctacccttt tgtcttcttt cataaaggta attaaaagag ctatacaaac accaaatatt
    21121 gcagctgcag taattgatat ttcaaaatat tgaaccataa aaaatcctat aataaacatt
    21181 ggtaaaaatt tatttttacc tatagtaaat attgttgttg caaaacctaa tgcaggtaat
    21241 ataccgccca ttactgttaa tccattcatt aaccattgtg gtataacatc taataatgat
    21301 tgaactaaat ctgctccaaa aaagttacat acaaaaacta ttggaaacct taaaacaaac
    21361 ccaaatatta aagaataaag tgttgcacat ctccagatgc cttttgtatt accttcttca
    21421 gcatacttat ctgccttatg aaccaataca gcattaccag ttcttcttaa gttattaact
    21481 aatactccta taacacctaa aggtaccgca atagatactg caacttctgc attaacacca
    21541 gtttgaagtg ctactggtat tgcaataaga gcagctagac atttatctga tggaatattt
    21601 ccccctgctg cgaccattcc tagataaacc atttctatac ttgcaccaac aatcatacca
    21661 gttgttacat caccccaaag taatccaagg ctaaaaccta taaataaagg agactttagt
    21721 gttgagaaaa gggtataccc tgcaagacct gctgcaaacc aataccataa tcctgttaca
    21781 attgccaaca tcaaaacaga ttccatttat aattcctcct aatttatatt ttttatttaa
    21841 atttctttaa cgcattttca tatgtcatag atgattcctc tggtataact tgtaggacta
    21901 tttcaacccc attttcatta agttcattaa gttgttctac atcctcattt cctaatgaaa
    21961 cagctgtaaa caccattttc tttcctgctt catttggaat acctcctaat tgaatctttt
    22021 catatttaag tccagcttta taagccttat ttgccatatc aacattttta aatagaataa
    22081 atatattttt atctcctaaa gtgttgtttt ggaatgcttg tcccgcatct tctgctgtta
    22141 aaataactac ttctactcct gatggtgctg ccattttata tatgtcagcc ataaattcat
    22201 cttgacctaa gaagtcatca actattatta ttttttgagc ttgagcgatt tttacccatt
    22261 ttgttataac ttgcccatga attaatctgt ggtctactct tactaatgtt atttttgcca
    22321 ttatattcac ctcataattt atattttttt aaattcttta attaaatcca cacaaaggtt
    22381 attatttttt ttgacgattc tttctggtaa tgtatgtaat ttatcttctt tgcttaattc
    22441 ttcatctacg attaaaattg tctgtaagct aagtccattt attgcatcta ttcctcttct
    22501 tatagcaaca gctattgcaa cacttgatgt agatcctcca tataaatctg atattataat
    22561 tttcacatct tctttatcta ctacttcttc aacctctttc ataaatacag aaacatcttt
    22621 ttcagaactt agtgataaaa cttcatattg atttgacaat ccaaagtttt tatttgtttc
    22681 ttctactaag acttttcccc aagttccatg gcacagtatg attaccttat atttattcat
    22741 tgctaaataa actctccttt atagcgatta atattttggt ttaattctac ttcttcttca
    22801 tcaggtacca tttgaattga tacattaata ttctttttaa taaattcatc tattaaaaga
    22861 aactcttttt tattaatgta tacattttga gatattttct tttttcctat tcctgtaggt
    22921 attcttccaa caataagttt tttgatatta atcccttctt ctatcattct atttgcagta
    22981 tctaaatttt taattaatac aaaaacgtta tcatcagctt gattattata aaaatatctc
    23041 cttgattcac ttacagttaa tattgataaa tcaatattct ttggtgctgc catttttatc
    23101 aaacctttag caataacatc attggttgtt tcatcatcta ctactactat cttttttaca
    23161 cctagatgct taacccattt atatataact tgaccatgta ttaatctatc atcacatctg
    23221 caatatttta ctaaagacat ttttaatcaa actccttttt ctattacatt gagctaagaa
    23281 ctttattaat attgtttata ccagtttgtc ctgcttcaat acatgcttct gctaactctt
    23341 ctaaactttt tccctctcta ttaattaaag cttcaagtag cattggaaga tttactccag
    23401 taagtgcatg aaacttgcca ccttttttaa ggtttaaact tgcaacatta cttggcgaac
    23461 ctcctaatag gtcagctaat actaaaactc cgtcaccttc atctaattca tttaactttt
    23521 ctacaaaatt tttatttaag tcttcaatat tatcattcct atttagttta attgctgctg
    23581 ttctttctgc tggtcctgca ataagttcac agctttttat aatttcttca ctaaaactcc
    23641 catgagtcat aactattatt cctatcacca atttcacctc acttttcttg tcacttttta
    23701 tataagcaat tgccgtgcca acttatgtgt atcaataaca aatggctatt ttacaggatt
    23761 taatcaaaac tttttactca atttttttta aaaacttatt atttcgatat gtatcaaaag
    23821 attttttcaa cctgtatcaa atctgtatca aaaaaagtaa aaaaaagata gccttttggc
    23881 tatttttaaa ttttgtatca acttttgaat ttatctgtat catattgtga ttcaaatata
    23941 tctaatatat ttgcatattc catatctgaa ataattatat cgaatgtctc ttctactagc
    24001 ttaaaacttt cttttataac taaatataat tctttatttc tactaattct ttcttcatat
    24061 gatttataaa ctaaagactc ttttattatt actctttcaa tcatacatga acaatgaaat
    24121 ataaacttta ctaaaagttc atcttgaatt actaaagaat agtgttcact aattctatta
    24181 attacttctt ttaatataaa ataagccttt tccccattta aaaaagtcaa agttttacta
    24241 atattatcaa ttagtaatct atcaaaatat ctaccaccat tatctttttc agcatatgta
    24301 acttccatat ctccataatg tttctttata gattctataa tattaaaata taatttctct
    24361 aatgtcattt ctggagttag agcctttcta gttgcttcta ttacaatagg tgtacttacc
    24421 atatctattg cttttacttc ttttcctgta gcatcacgaa taactttagc aaatgttaaa
    24481 atagacccca tatcagttaa aattaaaatt ccttttcctt tatcaatttt attggctatt
    24541 tctacagctt gtactaaaat atcctgaact ctatcttcta atcccatatt aattgccact
    24601 gcatggttac aatccagtaa agtattggct acattcacca tgctagatgc tgtagattca
    24661 ccatgcatta tgactaatat tgctatactc tcctcattta atcccacata cgataaataa
    24721 agaaatgttg ctaaaaaaat tgcttcatct tctggaaact ctactccata cattaatgac
    24781 atgttagata atatttcttt tgctatgtta tattcatatg ctctttcctt cataatttca
    24841 tctttattag gatgctttac tatatttcca atctttattc tttcaattaa attctgaata
    24901 tgtaaagcta tagcttttat taatttatct aatccttctc ttttgctcca tttatcatgc
    24961 ttttgtaaaa tatattttac agtagttgca attgaatcat taacaatgtt gttataagct
    25021 gtttgattac tactattata tataaaagta ttcattaaat tataggaata cttctgtatg
    25081 tctccatcta taacacttct aatttgtgac tctttaatac cttcttgttg aagtttattc
    25141 caagtttctt ttatcattcc ataaaaatct aaatcatatt ttttattttc tattaataaa
    25201 ttttcacttt caaaatcctt ttcttctggt aaaaaaataa tatcatcttt tccaataaaa
    25261 ctgaaatttt gtacaaactc ctcttttcta ttatttccat ataatgattc tctaatatta
    25321 tttggtagaa gagagagcct tacatataca cattcccttt tataggtcat ataatctaaa
    25381 aaagctcttg cacaaattaa ttgtatatcg ctttttaatt ggcctatgtt tcccttacac
    25441 ttatataaaa taaatgcctt aataacttct tttgaaacct ttaacttaac accaatccta
    25501 acactctctt ctttaaaaaa ataacatata agcttcattc tctcttgaaa gcctctttcc
    25561 tctaaagacg gaatctttat agttacaggt attcttctta aaaacgtttc caacattgct
    25621 gtttctgggt cttctgtagt ggctgcaatt atcataactc tacactcgtg taccttatta
    25681 gcctctccta accttctata tatcccttta tccataagca gaaatagcat ttcttgtccc
    25741 tctgcagata atctatgaac ttcatctaaa aacaatactc cattattagc ttcatgtaca
    25801 ataccatact tatctttatc agctccagta aatgctcctt ttacatatcc aaaaagctgc
    25861 gctaataata attgctgatt atcagaataa tcagcacagt taaatatgat aaatggtgca
    25921 ttatcactat cttcttttat agttaaacta tacttataca tatattctgc aaatatagat
    25981 ttaccaactc ctgtaggtcc tattattaat gtactcaacc ccttaggtgg atatattact
    26041 gctgctttag cctgttctat ttgggacctt aaacttccat cacatcctac taaattaaaa
    26101 atattgttct ctttttttat actctctaaa atatactcat ctttgataga atcattttca
    26161 ctatattcgt ttatagagtt aatattatca aatatccctt tatttagttt ctttcctaaa
    26221 atactctcaa tactttcttt atataagtac tgaacaggct taccctttat ctttataata
    26281 atactttctt ttattaattg atttaattcc ttactagcat tacttctatt tatacctact
    26341 tcagtagcta tatattctgc atcaaatcca aatctaatgc cattattaaa gttcaagtgt
    26401 tcttctgtta accttttcaa gcaaattaaa actttttctt tccttgatag cttcttttgt
    26461 gtcatagtta acctccccta taagcagtaa tactgttaaa catttattat taagcttaat
    26521 tctacaatat tttaatattt ttgtctaatt atgtaactat tgattttctt tcctattcgt
    26581 tttataatat aaattacgtt accctcatct tttatagagg aggaatttat atgaattttt
    26641 tatcagattt acacacccac tctatagtca gtggtcatgg ctatagcact ttactagaaa
    26701 atattaatta ttgtaaagaa aatggaatta agatactagg tacatctgaa catggtccta
    26761 agatgcctgg ttctccccac aaatggtact ttcacaacat tcaaaatatc ccaagaatca
    26821 taaataacat tataatttta agaggctgtg aagcaaatat actagatatt aaaggaaaca
    26881 tagacctaga gccttttgta attcctcggt tagattatct tatcttgtca tttcacgaag
    26941 ctgtgttttc tccaaataca ttagagaata atactaaagc tttaataaat gcaataaata
    27001 agcatgataa tattgaaata ttaggacacc ttggaaactc aaactaccca atcgactatg
    27061 aattaatcat taaattagct atagaaaaga atatcttaat agaaataaat aattgttcta
    27121 ttaaaggcgt gtcaagaaat ggtagctcgg ataactgtaa gtatattgct acactttgca
    27181 aaaaatatgg tgctaaaatc atactgactt cagatgctca catttgtttt gacataggta
    27241 actatgagta ttctgaaaat atcttaaagg aaattaattt tccagatgaa ttaataatga
    27301 attatcctaa aaaacttatt aatcattttc accaaaaagg taaattacta gatgttgatt
    27361 atacaaatat ctaagtaata atacatgctt actttaactc ccataaaaat tttaatacta
    27421 tttatattat atgaaataaa gaaattataa taaagaagct atttaataaa gcttaattac
    27481 ttttatgaac ttgatggatt gatatggtct atgatagcaa tagaaattat aacattttta
    27541 ataaacttag ttttatatac cttatagtat aaatctaaaa aagcatcatc taaaaaggag
    27601 ctgtcacatg agcaaattaa atttgttcta tgatagctct ttttttactt attttaatta
    27661 ggtatatttt taattatttt atataaagct tatgttctaa gtaaaattat gcataacaaa
    27721 caaaggtata aaaataccat tttttcagat aatatttaat tataaataat aaatttagaa
    27781 tacttctttt aaatgatgag agtctagaaa tttttatgtg tattctttaa ataaaaattt
    27841 ccagactcta gccccttttt gaatatttta aaatttaact taaactaagc aaattctata
    27901 atagcttatc tctaaatatt taaatttaat ttatcataca atattactta ttat
//
LOCUS       DAEOSP010000031        27335 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.031, whole genome shotgun sequence.
ACCESSION   DAEOSP010000031 DAEOSP010000000
VERSION     DAEOSP010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27335
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.031"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            150..1301
                     /locus_tag="KRH54_001088"
     CDS             150..1301
                     /locus_tag="KRH54_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBG8578757.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1347..2522
                     /gene="thiI"
                     /locus_tag="KRH54_001089"
     CDS             1347..2522
                     /gene="thiI"
                     /locus_tag="KRH54_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBG8578758.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSILPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2678..3481
                     /locus_tag="KRH54_001090"
     CDS             2678..3481
                     /locus_tag="KRH54_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG8578759.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYSPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3724..4365
                     /locus_tag="KRH54_001091"
     CDS             3724..4365
                     /locus_tag="KRH54_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBG8578760.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
     gene            5778..7448
                     /locus_tag="KRH54_001092"
     CDS             5778..7448
                     /locus_tag="KRH54_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HBG8578761.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(7570..8304)
                     /gene="pflA"
                     /locus_tag="KRH54_001093"
     CDS             complement(7570..8304)
                     /gene="pflA"
                     /locus_tag="KRH54_001093"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBG8578762.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(8464..10695)
                     /gene="pflB"
                     /locus_tag="KRH54_001094"
     CDS             complement(8464..10695)
                     /gene="pflB"
                     /locus_tag="KRH54_001094"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBG8578763.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            10988..12049
                     /locus_tag="KRH54_001095"
     CDS             10988..12049
                     /locus_tag="KRH54_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBG8578764.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            12140..13753
                     /locus_tag="KRH54_001096"
     CDS             12140..13753
                     /locus_tag="KRH54_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG8578765.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            14028..16013
                     /locus_tag="KRH54_001097"
     CDS             14028..16013
                     /locus_tag="KRH54_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG8578766.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKVKSFDEFYKIITKRR"
     gene            16018..16452
                     /locus_tag="KRH54_001098"
     CDS             16018..16452
                     /locus_tag="KRH54_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBG8578767.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            16481..17041
                     /gene="srlA"
                     /locus_tag="KRH54_001099"
     CDS             16481..17041
                     /gene="srlA"
                     /locus_tag="KRH54_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG8578768.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            17105..17473
                     /locus_tag="KRH54_001100"
     CDS             17105..17473
                     /locus_tag="KRH54_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HBG8578769.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            17498..18064
                     /locus_tag="KRH54_001101"
     CDS             17498..18064
                     /locus_tag="KRH54_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG8578770.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            18105..18470
                     /locus_tag="KRH54_001102"
     CDS             18105..18470
                     /locus_tag="KRH54_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG8578771.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            18571..19344
                     /gene="srlD"
                     /locus_tag="KRH54_001103"
     CDS             18571..19344
                     /gene="srlD"
                     /locus_tag="KRH54_001103"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBG8578772.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            19692..21962
                     /locus_tag="KRH54_001104"
     CDS             19692..21962
                     /locus_tag="KRH54_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG8578773.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMALHKVNYISDELD
                     TTSNKVNYVSDELDITSNKVNYVSDELDMTSNKVNYVLDELDITSNKIDYISNELYNL
                     SNKSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKVCFDMFITDTATLCDMF
                     IPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFMELAKKMGLNDYPFVE
                     KRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKFETPSGKYELYSESIKN
                     LGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKKSIAQAYINRRMAKK
                     VGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHGNPNSLTDTGISDFG
                     GQVTYNESMVEIIRQN"
     gene            22050..22385
                     /gene="spoIIAA"
                     /locus_tag="KRH54_001105"
     CDS             22050..22385
                     /gene="spoIIAA"
                     /locus_tag="KRH54_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBG8578774.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            22399..22827
                     /locus_tag="KRH54_001106"
     CDS             22399..22827
                     /locus_tag="KRH54_001106"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBG8578775.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            22850..23614
                     /gene="sigF"
                     /locus_tag="KRH54_001107"
     CDS             22850..23614
                     /gene="sigF"
                     /locus_tag="KRH54_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBG8578776.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            23894..24331
                     /gene="spoVAC"
                     /locus_tag="KRH54_001108"
     CDS             23894..24331
                     /gene="spoVAC"
                     /locus_tag="KRH54_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBG8578777.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            24485..25498
                     /gene="spoVAD"
                     /locus_tag="KRH54_001109"
     CDS             24485..25498
                     /gene="spoVAD"
                     /locus_tag="KRH54_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBG8578778.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            25512..25874
                     /gene="spoVAE"
                     /locus_tag="KRH54_001110"
     CDS             25512..25874
                     /gene="spoVAE"
                     /locus_tag="KRH54_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBG8578779.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            26057..27073
                     /locus_tag="KRH54_001111"
     CDS             26057..27073
                     /locus_tag="KRH54_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG8578780.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
BASE COUNT        10723 a         2968 c         4812 g         8832 t
ORIGIN      
        1 ttgtaataaa gtgaaaaaac tagcttttca tgaaataata ttttataatt taagattgtc
       61 tttttaataa agattaacaa aaaaaatact ataattagtg gtataatagt tagagttgaa
      121 gattttaaat aaaggtaggt gtaattaagt tggaaatata cttagacaat agcgcaacta
      181 caaaacctta tcaagaagtt atagataaaa tggtttatgc acttaatacg gaatatggaa
      241 atccttcttc tgtccataga aaaggtgtag aagttgaaaa agctataaaa gaagtaagac
      301 aagatatagc taaatctcta ggagcgaaag aaaaggagat ttattttaca tcaggtggta
      361 cagagtgtaa taatactata ataagaggta ttactagttt aaataaaaaa agaaaaaatc
      421 acataatatc tactaacata gaacatcctt ctgttttaaa tacattaaaa gatttagaag
      481 aagatggttt tgaagtaact tatttagagg tcggaaaaga tgggaaaata aatatagagg
      541 atttaaaaaa tgcgataaag tcaactacat gcttagtaag catgatgcat gttaacaatg
      601 aaataggaac tatacagcca ataggtgaag ttggaaagta tttgaagggt ttaaaagaaa
      661 aaatttattt ccatgttgat gctattcagt catatggaaa aattaacttt agaccatcta
      721 aatacaatat agattttatg agtgtaagtg cacataaatt tcatggtcca aagggaattg
      781 gatttatgta tataaaagaa aataatcgcc ttaagcctat gttaacaggt ggtggtcagg
      841 aaattggaat taggtcggga acagaaaatg tacctggaat atatggtctt ggagaagctg
      901 ttagaatact aaacaaagat ttagatgctg ttatttcgaa agtaaataac ttaaaaaata
      961 tattaaaaaa tgaaataatt gataatatag aagatataaa aattaattct ccagaagatg
     1021 gagtatgtca tatattaaat gtatcattta gagggacaaa aggagaagtt ttacttcatt
     1081 acttagagca aaaaggtatt tatgtatcaa ctggctctgc atgttcttct aaaaagaaag
     1141 gtagttacgt attaaatgct ataggtctta caaatgaaga aataaatgga acaataagat
     1201 ttagtttatc tgatatgaat acagaagaag aaatgttaga agctgtgaaa gttttgaaag
     1261 aatcaatttg tgacttaagg tcaataatga aaagaaaata aagaatactg tatagatagt
     1321 gataataaat tagtttaaga ggtgaaatgg tgtataatat attaatagta aagtatggag
     1381 aaattggagt aaaaggaaag aatagatata tatttgagaa taggcttata agaaatataa
     1441 gaaatatgtt aaaaccaatt ggaagattta atgtatataa agagtatgga agaatatatg
     1501 ttgatttaga agactatgat tatgaagaag taattgaaga agttagaaaa gtatttggga
     1561 tagtgggtgt gtgccctgga gtaagagcta aaaaagatta tgatacatta aaagaaatag
     1621 ctttaaaaat gttagaagaa aaaatagagg ctggatataa aacttttaag gtagagtcaa
     1681 gaagaggaga taagtctttc gggttgactt ctcaagaaat gagtatggat ataggtggat
     1741 acttattatc aaaggtagga gataggatta atgttgatgt aagaaatcca gaagttaaaa
     1801 taaagtgcga atacagagaa tttcatacta tggtctatag tgatactata cctggatatg
     1861 gtggattgcc acttggaacg aatggtagag ctatgtctct tttatcagga ggtatagata
     1921 gcccagtagc tacatggatg gttgcaaaaa gaggtatgga agtagaggca gttcactttc
     1981 atagttatcc atttacaagt gaaaggtcac aggaaaaagt taaggattta gctaaaatac
     2041 tggcaaaata ctgtggtaga gtaaggcttc ataaagttaa tatcttagaa atacaaaaag
     2101 caattggcga gaattgtaat gaagaagaag ctactattct ttcaagaaga tttatgatga
     2161 ggatagcaca gagactttct gaaaaaagac attgtgatgc tttaattaca ggtgaaagta
     2221 ttgggcaggt tgcatctcaa actatacaag gacttacttg tactaatgcc gttgtagact
     2281 taccagtgtt tagaccactt atagcaatgg ataaatcaga tatagttgat atagccaaaa
     2341 aaatagggac ttttgaaaca tctatattgc ctgaagaaga ttgttgtagt gtattttctc
     2401 ctagaaagcc cgtcactaaa cctagattag agaaaataga aaaatctgaa acagcacttg
     2461 acattgaaaa attagtacag gatgctattg acaaaataga ggtagaggat attgagtttt
     2521 agcatacaag ttatttaatt actaaattga atattgagta tattagtagt tctgtaagta
     2581 tattagtagt tctgttgaaa ggatttatgt agacaattta ttaatatgta gtatttaaaa
     2641 ataagtacta taattttatt taatgggaat gattattatg aataaaaagg gttttacact
     2701 aattgaattg ttagtagtta tatctataat aggcatttta gttatagtag ctgttccagc
     2761 attgtttaga aatatagaaa aaagtaaagc agttacatgt ctttctaata gagaaaatat
     2821 aaagactcaa attgttattg caatggctga ggaatcaagt aaaggcaaga atgaagtcat
     2881 gaaagaggta ttagaaaaca aagatggtaa gtactttgaa acagaaccaa agtgtaagtc
     2941 aggtggaata tattcagcaa cgtttgatga tggttatgat ggaataactg gaatagaaag
     3001 cattgcaaaa gtgtatgtta cttgtacaaa acatccagac ggtgttgaaa tggctaggga
     3061 tatacatcaa agtatgaaag atttgattgc atcatttgca caagaccctt ctataatacc
     3121 aggagcttca aagggtaatg atgattttag aaaatattta ttagacaata aatataaaaa
     3181 tgggtggcct acaattccag atgaatttaa ggcaaaatat ggattaagta aggatacact
     3241 atatatacaa ccatatgcat atagtcctac taaatctgat gctactgtag ttgtatttgc
     3301 aaataataag actgggggta attggtatac ttccctagtt tacgattatg atgaaggtag
     3361 atggtataaa ggtaaaaatg gtatttctgt tgcaggtagg tcatgggatg ttgacacaga
     3421 tagtgttaag tctgtaaaaa cagagattca ttctaaagag ggatggggtc ctttaaatta
     3481 atatatcatg ttttattaat ctggtattga ttaaactata gaaaagagca gtttctatag
     3541 atgacgaata ccagattttc atttatatat ataattgaga tatattaaaa gtgtttatat
     3601 ttttaagtct tcacatacaa gctaaaaata attataataa tattgtaaag aaatttaatt
     3661 ataagattaa ttttctgaag atttagtatt tgtactaaga aatacataaa aaggggaata
     3721 gatatgaata ataattttca agacaaccaa acaataaatt taatacaaag tcgtagaagt
     3781 atacgaaaat ttacgacaga acaaattagt gatgagcaag taaacacatt attacactgt
     3841 gcatttgcag caccttcagg atgtaataaa cagccttggc acataactgt tgtgcaagac
     3901 cagaaacttt taaaagagat tagtgatgat actttatcta ggattcatga ggtgagtaat
     3961 gttgagataa ataaaaactt taagttattt tatggtgctc caaccgtttt atttatttct
     4021 tatgatgaaa gtagttcatg ggctccatat gatataggaa tacttacagg gaatataaca
     4081 acagctgcac aagcactagg gcttggaagt tgtataatag gaatggttag aggtttattt
     4141 acacctgttg aacaagggga tattgaaggt cttgtgagtg tattggataa agaagatgta
     4201 aaagaatcag agtcaataaa gatgaagttt gatacaaata aaaaatacag agaactactt
     4261 gatataccag aagggtattc tgtaccattt ggtattgctg taggtattcc agatggaaat
     4321 ttaccaaatg ctagagaggt agtttacaaa gtttccagag tttaggataa agtattgcaa
     4381 gaaccaatca gttacttgaa gaattaaacc agtaatatac aaataaattg atggaagagg
     4441 aagctattta aaaagtagtt tagatttttc gttgtaaaat aattaaataa agaataaggt
     4501 gataagtatt ttatttatta tcttattctt ttttattttt gttttgtaga atgataaaaa
     4561 taaggattaa gtgtaataaa atagaaaaca agtatgttaa ttttaagtat atgatttaaa
     4621 atttatccaa gttaaaaatt taagatatct tttcagtata atggaaaaaa tgtcaaaata
     4681 ttgaaatagg gaaaaaatag tgatataata agaaaaaata gagaaaaaaa gtaaccctta
     4741 gttataaaaa agttactatt tattgaaaat ttagatattt ttctaaaaat tatctatgtt
     4801 aatagttaaa tttatgaaca ttttttgttt ttatgttagg atatttctta gaaatgaaat
     4861 acttcttaat ttgatttgat atgtatattt ttatagctat tttattataa aaaaaaagaa
     4921 cccttttata acaaatggaa actatggatg taataattaa aataaaaaat attattttat
     4981 atttataaac attgacattc taattttgta gtgtaatcgt attttttatt aattcttaca
     5041 aaatcaatga aaataagtat atccatttta aagtacataa atttaaaaat ccagaaaaat
     5101 atacttaaat tatgtaagta aaagaggggt tgaatcagtt gaaaatgtaa tattagtaaa
     5161 tagtacatca atcctatata ccctgtaagc aactctattt tcatgtacta aaaatgtacc
     5221 tattttatta actgaaaaga ataaattaaa tgtaagtaca taagaagata gaaaaaatta
     5281 aaaacaaaaa atatttatat aaataaatat aaaaaatgat ataatcccta ttaaaaaatt
     5341 tatagatgat gaagaaatat caaaatctta tattatagga agagacactt ctttgccaaa
     5401 atctatagaa aagcaatacc aaatccaaag agaataggcg aagaaaaaaa taaaataaat
     5461 tcaagagtaa tagaaaagtt ttatagaata aagaactgat aactctttat ataacgaaaa
     5521 atagaatttt aagagaaaat gatttaataa atgatttaac atggtgtttt agcaggtaaa
     5581 aataaatcac cagtaatgat tatagaaaaa ttactttatg aatctcaaaa aacttagtta
     5641 agagtaagag atttgataag ataactaaag ttggggaaat gtgtttgatg agacaaaaaa
     5701 ttagtaaaat agtataatct aaataaggat attttgttga ttaaactaca ctaatagtaa
     5761 tattgtaggg ggaaaagatg agagaagaat cgaactcttt aagtttagac catatagaaa
     5821 ttttctttga acttttatct caaagtacag aagattatat atttttttgg gatataaata
     5881 ggaataaatt taaaatttct tcagcaattt ttgatgaatt taacttaagt aaagagatag
     5941 aatctgattt ggtcaattgt tggagtaaaa ttgtttatcc ggatgatgtt caaatatgga
     6001 aagatgatat acaagaatta ctacatggaa aaaaaggtga gcacaattta gaatataggt
     6061 tgattaataa atataaagaa atagtatgga tttcttgtag aggtaaagtt tatgtgtcgg
     6121 atgacccaaa aactatattt ctagtcggga gaataaagaa tattggtgaa aaaaataaat
     6181 ttgattctat cacaggaacg tggaatagag aacaatttga acatagaatg aattatctta
     6241 taaaagagaa aatttataaa aatggtgcta tgtttattat ggatattgat aattttaaaa
     6301 atataaatga aaaatatgga cattcttatg gtgacaaagt attgcgtgca attgctactg
     6361 aagtattaga atatcttcca aaagatgtaa gattgtatag gttagatgga gatgaatttg
     6421 cattttttta ccctatgtgt actaaagaaa ctatagagaa aatatatgaa aagatacaga
     6481 tgtatacaaa tactcagcat gaaattgaat caaataagta ctattgcaca gtgactgcag
     6541 gcgttgctat gtatccagaa gatggagata attatttaga tttatttaaa catgctgata
     6601 ttgcattgga tatagcaaaa attagtggta aaaatagaat taagtttttt tcacaagagc
     6661 tctatgaaaa taaactaaag gtaatttcaa tgcaacaaaa actaagagag tgcgttgaaa
     6721 ataattttaa tgactttgag ctgttttttc aaccccaggt aaatgctgtt acaaaagagg
     6781 taataggggc agaggtactt ttaagatggc attcgagtac ttatggagaa gtatctcctg
     6841 ttgaatttat accaatacta gaacagagta atttaattat accagttggg aagtggatta
     6901 taaaagaagc tgtaaaacag tgtaaagaat ggcataaaat aaatccagac tttaaaatat
     6961 ctgtaaatgt atcgtatatt cagttaaagg aagatttttt tagagatttt atagtagaat
     7021 gtttagtaga gtatcaatta agacctgaat ttttaatttt ggaattaaca gagaattgtt
     7081 ggataccaga tataaattta ctaaatgata aatttattag tttaaagggt ataggagtat
     7141 atattgcaat agatgacttt ggtacaggat attcatcttt aaattacctt aaagaactgt
     7201 ctgttaatat aataaaaatt gaaagaagtt ttgttaagaa tattacatat aatagttatg
     7261 aatatacttt tcttgaatat attattaaat tagctcatat tataaattta aaagtttgtg
     7321 ttgagggaat agagtcttat gaagagtatg atatagtaaa aagcttaggg gtagatatta
     7381 ttcagggttt tttatttgga agacctgtaa gtgcctctga attttataaa tttcaactat
     7441 caaagtgatg tatataaaat gagatatata gtatataaaa atagggcaaa gtggaagttt
     7501 tgaagtttat taaaaattta cactttaccc tattttgtta tgtcttctat aaaggattaa
     7561 tatactacac taactattat atactttcac tccaaattta gagattattt cactagcttt
     7621 ttggacatct tccttagtgg catcaggaac atcttttagt tcataatcga tacctaaact
     7681 ttcccatttg tgtattccca tagaatggta tggtagcaat tcaaatcttt ctacattttt
     7741 caatgtagct acaaattgac ctagtttttc taaattatct atatcatcag taatgccagg
     7801 aactaaaaca tgtcttatcc atacaggaat atttcttgat tctaagtgtc tagcaagttt
     7861 taatgcctta tccataccaa caccagtcaa atcaatagat ttttcctcaa ccatatgttt
     7921 taaatcaagt aaaaccaaat cagtattatc taaaactggg tctatagttt ctatatctac
     7981 aaatccagat gtatccaagc aagtgtgtat atcattttct tttgcttttt taaataattc
     8041 acttaaaaat tctggttgaa gagtggattc accaccagag gcagttatac ctccaccaga
     8101 aaacttcata aaagaagtat acttaagagc atccgttatt atttcatcaa ctgtgtattc
     8161 ttttcctgat ttaatatccc atgtatccct gttatgacag tatttgcatc taagaggaca
     8221 cccctgaaaa aataatatat atcttattcc tggtccatct acagtaccaa acgtttctat
     8281 agaatgtatc tttcccttaa tcatagagtt cacctcacaa tatattttaa taaccttatc
     8341 aatagttata atatttctct aaaaaaggcc atcttatacc agaaataatt caaatataag
     8401 atagcctttt atttttattt tttacacagt agtaatctta gttaaagtat aaaaatttaa
     8461 agactaagcc attttaccgt ggaatgttct gtttataaca tctaattgtt gctctttagt
     8521 taattttatg aagttaactg catatccaga tactcttata gttaattgtg gatattcttc
     8581 tggatgttcc atagcatctt ctaaagtagc tctatcaaat acatttacat ttaagtgatg
     8641 agcattttga gcgaagtatc catccatcat tgcagaaaga ttgtttattc tttcagtcat
     8701 atcttttcct aaagcagcag gtactataga gaaagtatta gatataccat cttgagcatg
     8761 ttcatatggt aatttagcaa ctgaagataa tgaagctaaa gcaccattag tatctctacc
     8821 atgcattggg ttagcaccag gagcaaatgg agcaccagct cttctaccat ctggtgtatt
     8881 accagttttc ttaccataga caacatttga agttattgta agtatagatt gagtaggata
     8941 agaatttcta taagttttat tctttcttat tttattcata aagctttcaa ctaactctac
     9001 agctatacta tcaactctat catcattatt tccgtatttt ggataatctc cttctatttc
     9061 aaaatcaaca gctattcctt gttcatttct aatagtttta accttagcat gttttatagc
     9121 agataatgag tctgcacaaa ctgataatcc agccatacca caagccatag ttctaaatat
     9181 atctctatca tgtaatgcca tttctaatgc ttcataagaa tatttatcat gcatataatg
     9241 tattacattt aaagtattta catataatgt tgctaaccaa tcagtgaatg gttcaaattt
     9301 actcataact tcatcataat ctaagtactc agaagttata ggttcaaatc ttggtccaac
     9361 ttgaacacca gatttctcat ctacaccacc atttatagcg tataataaag ttttagctaa
     9421 gttaactcta gctccaaaga actgcatttg tttaccaatt ctcattgcag atacacaaca
     9481 agctatacca tagtcatctc cccagtatgc tctcattaaa tcatcatttt catactgtac
     9541 tgaacttgta tctatagata ctttagaaca gaaatctttg aatccttgag gtaatttagt
     9601 tgaccataga actgttaagt ttggttctgg agaaggccct aatgtatata gagtatttaa
     9661 cattctaaat gagtttttag taactaatgt tctaccatct attcccatac ctcctataca
     9721 ctcagttacc caagttgggt ctccagagaa taagttattg taatcaggag ttcttaggaa
     9781 cttaaccatt cttaatttca taacaaaatg gtccatatac tcttgtaatt cttcttctgt
     9841 tacgattcca gcttttaaat ctctttcaaa gtatatatct aagaaagtag atgttctacc
     9901 taaagacatt gcagcaccat tttggtcttt aacagcacct aaataaccaa agtataacca
     9961 ttgtacagct tcttttgagt tagttgctgg ttttgatata tcaaatccat aagattcagc
    10021 cattttttta agttcattta aagctgatat ttgctcagtt atttcttctc ttaatctgat
    10081 aacttcctca tccatgcaag aaacttctaa tgatttcttt tgctcatttt tatcttctat
    10141 taaagcatca acaccatata gagcaactct tctgtagtca ccaattattc tacctctacc
    10201 ataagcatct ggaagacctg ttattatacc agattttcta gcagctctca tttctggagt
    10261 ataaacatca aaaactcctt ggttgtgtgt ttttctgtat ttagtaaata tatcttttat
    10321 ttctgggtct aattcatatc caaaagcttc gcatgaattt tcaaccatct ttataccacc
    10381 atatggcatg acagctcttt ttaaaggggc atccgtttgt aacccaacta ttgtttctaa
    10441 ttctttatct aaatatcctg gagcatatgc agctattcca gaaacagttt ttgtatcaac
    10501 atctaaagta ccaccatttt ctctttcttt tttaaatagg accattgctt cgtcccataa
    10561 ttttttagta ttttcagtgg caccagctaa gaaagagtca tttccctcat atggagaata
    10621 gtttaattgt ataaactctc taacatttat ttctttagtc catcttcctg ttttaaatcc
    10681 ttgccatgca ttcattaagt atttcctcct tagatttaaa tgataaataa tactattaaa
    10741 attaattttt atatacttaa tttattttat atattttttt aaaaatatat tttctaatat
    10801 aataatagca tattattcgt atcctgtata cataaaaaat aaaaaattgt cgctttaaag
    10861 tataaaacag attatatgtt aataatgtgg tgaaattata cccatatata aataaaataa
    10921 aacaaataaa tagctaatta aggagaaaat attttttatt ttttgtttag gataaataaa
    10981 ttttattatg gaaaaaagaa aaagcataca tcttcacaaa ctgaatattt atttaatagg
    11041 gggaggtgat agattgttta tcacagtggt attattttta gtaggtttcc tattaattac
    11101 taaaggcgca gatatcttta taaattgtac tgtagagata ggtaaaaaaa cgaatatatc
    11161 agaaattata cttggagcta ctatagttag ttttgcaaca acattacctg aatttacagt
    11221 gtctctactt gcttctattg atggtcatac tactatgagt ttaggaaatg cagttggttc
    11281 tataatatgt aatacagggt tagcattagg gttggttgtt tttataagtc catttaatgt
    11341 agataaaaaa atgttttttt ctaagtcttt actattaata gtatccgtta tagttttaat
    11401 tttattgagt ttagatggag ttataacaag aggtgattca ttattactta taataatatt
    11461 aatcttttat atggttaata attatagaag tgtcgttgga aaatctgata ctaaaaatag
    11521 aaatattaaa aataacacta ttaaagacat atctactaaa aaagggaaaa attatagagg
    11581 tttttccgta ttagaaattg taaaaatact attacttttt gctacaggat tgataatgat
    11641 gataataggt tcacaaatcc ttatagagag tggagtaata atagctagtt ttttaaatat
    11701 acctcaaggt atagtaagtt taactataat agcacttgga acttctctgc cagagatagt
    11761 ttcgtcgatt actgctataa gaaaaaatca tcatgaaata tcagtaggaa atattttggg
    11821 tgcaaacata ttgaatatag tatcagtaat agctgtatca gcaataccaa ataacatacc
    11881 aatactatct caaaatagac aattggatat accattcatg atattattat tattaatagt
    11941 aataatacca acacttaaat ctaataaact tagtagaata caaggtatat tgatgttatt
    12001 cacatatttt ctgtacatat caattttgta tttcatgtat attatttaac tatactgttt
    12061 aaaatattaa ttgcagaata tcctaaaaaa gtatataatg gataggttaa gattaattga
    12121 taacggagag gaataatata tgaatataac aaagtttgaa gatttaccaa taagtgaggg
    12181 aataaaaaaa gctatagctg aaatggggtt tgaagaacca tctcctatac aagcacagtc
    12241 tatacctgcg attttgtcag gtaaagacgt tataggacaa gctcaaactg gtacgggaaa
    12301 aacagctgct tttagtatcc ctatactaga aacaatagac ccaaataata gaagtttaca
    12361 agctgtagtg ctttgtccaa caagagaact ggcaatacaa gtatcaacag aaataagaaa
    12421 gttagctaag tattcgcacg gaataaagac attacctata tatggtggtc agccaataga
    12481 tagacaaata aaatctctaa agagtggtgt gcaagttgta ataggtacac caggacgtac
    12541 aatagaccat atcaatcgta aaacattaaa gatggataat gtaaaaatga ttatattaga
    12601 tgaagcagat gaaatgctag atatgggatt tagagaagat atagaaatga ttttaagtaa
    12661 gattccagaa gaaagacaaa caactttctt ttcagctact atgccaagag gaattttgga
    12721 attaactaag agatatcaaa aagacccaga gcatataaaa gttgttagaa aagaacttac
    12781 agtttcaaat acaaagcaat actatataga aactaggtca tcaaataaat tagaagtatt
    12841 atgtagatta gtagatgtat atgaccctaa actatctgtt gttttctgta atactaagag
    12901 aaaagcagac gaattggtag gagatttaca agcaagagga tattttgcag atgcattaca
    12961 tggtgactta aaacagactc aaagagatat agtaatggat aagtttagaa atggtacaat
    13021 agatatactt gttgctactg atgttgcagc tagaggtata gatgttgatg atgttgaatg
    13081 tgtattcaac tatgacttac cacaagatga agagtattac gttcatagaa ttggtagaac
    13141 tggtcgtgct ggtagagaag gtatgtcatt tacttttgta tttggaaaag aaatgagaaa
    13201 gatgaaagat atagaaagat atactaaatc taaactaata aagcataata taccaacaat
    13261 aactgatgtt gaagagaaaa aagtaggtac tttctttgca caagttaaac aaactataga
    13321 agaaggtcac ttaactaaac aattacaatg gttagaaggt ttctgtaatg atgaagatta
    13381 tgcaatggtt gatatagctg ctgcacttgt aaaactatct ctaggtgaag aaatgaaaga
    13441 agaaataata gaagaaaaac ctagaagaga gcgtggagat agaaaaggtg gtactggagc
    13501 taaagatggt atgattagat tatttatcaa tattggtaga aatcaaagag tacaagcaaa
    13561 agatatagtt ggtgccatag ctggagaagt tggaatacca ggtaaagtag taggtactat
    13621 agatatatat gataagtata cttttgttga aatacctaaa aaagatgcaa aaacagtaat
    13681 tgaaaagatg aaagatatta agataaaagg taataagatt aatatagaaa aagcaaataa
    13741 gaagaaaaaa taacgtatag tatacaaaac tataattaga aaatatataa aataatatat
    13801 tggatattta aaaagcctga tgtgttttca aaacagatag tagataatct gtaagagtta
    13861 acagcataag gcttttcttg ttttatatga tgttataatt ttccagattg ttgtgaaaaa
    13921 tgtatattag ttcctttttt tcaaatctga tttgaaaaag agaatattaa ttatctatat
    13981 agtttagatt ataataaaaa atgtaagata acaaagggga gaaaaagatg tcaatgactt
    14041 atatggaccc tagggttttt ttgattttag aaatggtgtt agaagaagac acaaatactc
    14101 tagaacaatt ttcaaatgtt ttgggtgtta gtactaggac tatcagaaac tatataaacc
    14161 agattaatcg agaattaaaa ggtggtattg ctaaaatagt taaaaatgaa aaaggtacat
    14221 atagtataag aattgaggat gaaagtaaat taattgagat tgtaaatttt aatagaggaa
    14281 agctatcaaa ttttataaat ttaaattcac ctgatgaaag aataaattat gttcttgata
    14341 ttttaactat gaccaacaat ccaattacca tagatgattt ggctgaagag atatgtatag
    14401 gaagaacaac attaataaag gatttaaaaa aagcagacag aattttagct gaatatgatt
    14461 tagaattaaa aaggaagcca aatataggta tgattttagt tggtgaagaa atggatataa
    14521 gattattaat ccttgataga ttgtatgaaa attatattga tgtcttggaa aacgttaatc
    14581 agataaattt agttaaagat tatgatattg aatgtcttag agatgaatta aaaaagttat
    14641 ttaaaaaaga agaactctac ataacagaac aggtcttgag agatgttgaa agatatataa
    14701 tcatgtctgt tttaagaaat ctaaatgggt acaaatttga aaagatagat aagagatttg
    14761 aagtgataag atgttcagat gaatatactt taggattaaa gctcaaagct ttattggaaa
    14821 agatatttaa tatagtttta aatgaaaaag aaaccatatt tttgacaatg ccattaatag
    14881 gtagaaaagc tcctagtaca aaattagcac ttagcagtat aaagataaat gatagcgtaa
    14941 aggaagttgt agatgaagta actagttttt tattagagac ctcagggatt gattttaaag
    15001 aagataaaaa gcttatagaa aatttggaat atcacttgta ttttgcactt aatagaatga
    15061 gattcaacat aagggtaaaa aatcctcttt tacaagaaat aaaggaaaga tatacatttc
    15121 catatagcgt tgcaaagata gctgcaatga taattgagga aaagtttgac ttgaaaatat
    15181 gtgaccacga aataggatat atagcattac attttggaag ttattttgaa aaaaattcaa
    15241 gaaaagttct aaatgtaaat agggtagctg ttgtttgtgg aacaggtctt gggactgcac
    15301 aacttattag aataaagata ggaaaaatac ttggagaaag tgtagaagta aatacttttt
    15361 ctgatataga agctaaagct aatttagatt tattaaagta ttatgattta gtatttacaa
    15421 cagtagattt caaaacagac ttagatgttc caatttttag agtaaatgcg ttatttaatg
    15481 aagaaagtat aaagaaagag atagaaaatg caatactttt gaaaaatagt ataaatctta
    15541 agtctataaa aaagattgaa aaaaatatcc cttttatagg taaattaatt gaagaggata
    15601 agttctttat attgaataag agtacgttta tggaaaattt agaagatatg ttagaaaaat
    15661 tagttcataa aggaattatt gatatagagt tcaaaaaaaa ggtattaaaa agagaaaaga
    15721 aatcttcaac agcattagat aattatatag cattgcctca ctcagttaat actaatgggg
    15781 aaaatcttta tctggcattt ggaatattag aaaaaccagt gatttgggat acaaaggaaa
    15841 ttagaataat aatattgatg ataattccag ataagaatgt agatagtaca gacctgataa
    15901 taaaaagtta tgaagaagta cttgaagtag ggagagataa aaagatggta gaagaacttg
    15961 ctaaggtaaa atcatttgat gaattttata aaataattac caagaggagg taataatatg
    16021 gacccagtaa tttttattat agcatcactt attacagtgt atttattaaa tatttttctt
    16081 ggatacttac agcttaaaga ttttaataaa aattatattg aactaaaaag aaaaggaaga
    16141 gtggcaattg gcagaaaaaa aggaagaatt tcatcgggta caattgtgtt gatacttata
    16201 aatgatgatg gtgtgattgt cgagactaga aaaatgcaag gtgttacagt attggcaaga
    16261 gtaaagtcat ttgaaggttt ggtaggaaag agcttagagt atatagaaaa atcagattta
    16321 actgtttaca ataaactact taaaaaagca atactagatg cagtcaaaca gtacataaca
    16381 tttgagaaaa ataaatgtga agatacaaat aatctgagta ctgatgaaga gtgtaggatg
    16441 gaagtagttt aaaaaaatat atttataagg aggagttaat atgacgcaat tttttcaagt
    16501 attagcaagt ggtgcagata gttttatgaa tttatttagg gcaggtgggc aacaatttgt
    16561 aggttttgta acagatatag taccactttt agtagcatta ttagttacaa tgaatgcaat
    16621 tatcaacttt attggtacag atagagtcga gagacttgca aaaaaatgtt ctgcaaatat
    16681 atttactaga tatttgatat taccagtcct tggtacattt gtatttgcaa acccaatgac
    16741 actatcatta ggaagattct tgccagaaaa gtttaaacct agttactttg cagcagcaag
    16801 cttttcatgc catacaatga atggattatt cccacatatc aatccaggtg aactatttat
    16861 atatctagga attgcaaatg gtataacaac tttgggattc tcaacagcag atttagcagt
    16921 tagatatttg ttagtgggta tagttgcaaa ctttattaaa ggtgtagtta ctgactatac
    16981 aacaaaattt gtagagaaac aacaaggaat agcattaaat tcagatataa agattggata
    17041 attactttaa ggtataaata aatttttcaa ataaaaagat agtgtataga ggaggataaa
    17101 ttatatggca tttaacaaag ttaaaatagt aaaaggttct ggaggatggg gtggaccatt
    17161 gataatagaa ccaacagaaa agaagaataa ggtagtatat gtgactgggg gagcaaagcc
    17221 agaaactgca gtaaaaatag ctgaacttac aggatgtgag ttaatagatg gattttcaca
    17281 gggtgttaaa gatgatgaaa tcgcatgtgt aataataaat tgtggtggaa cattgagatg
    17341 tggtatatat cctcaaaaga aaataccgac tataaatata atgaaaacag gaaaaagtgg
    17401 accattagca atgtttataa aagaagatat atatgtttca ggggtaaaac caacaaatgt
    17461 agagcagtta taattgaggt agtaggaggg aagaaatatg gcttatgata ctaaaaagaa
    17521 gataagtgag cagagtcaag gaattgttgc aaaaataggt atagcactag gaaatgtgac
    17581 aagtactctt tttcaagctg gtagggatac tatagataca ttaatacata ctatactgcc
    17641 ttttatggct ttcgttgcag tcctaatagg tataataaat caatcgggta taggaaattg
    17701 ggtagccaat ttacttactc cattaatcgg aaatgttgga ggtttagttg tcttatcaat
    17761 aatatgttca tttccgttgt tgtctccgtt tcttggacca ggagctgtta tagctcagat
    17821 agtgggagta ttggttggta cagaaatagg aaaaggaaat attccaccaa gtttagcatt
    17881 accagcattg tttgcaataa actcacaagc agcttgtgac tttataccag taggtcttgg
    17941 tcttgcagaa gctgaacctg atacagttca agtaggagtt ccgtcagttt tatattctag
    18001 atttttaaca ggtgcaccaa ctgttctatt agcatggtta gcaagctttg ggttatataa
    18061 atagaagaat actttataaa taattagaga gaggttcagt tgatatggaa ttaatataca
    18121 gtactcaaat aaataaaata ggagataatg caaatgaatt tttacaacat aatatgtttg
    18181 taactttcaa agataatgct cctcaagagt tagaaaatta ctgttatata cattctgaaa
    18241 ataatttagt aaaagaaata cttacggatg atgtgctatc aataaataat gttgattaca
    18301 aaattacttc tgttggtgag ttagtaaacc aaaatctttc agaattaggt catataacat
    18361 ttaagtttac aggtgaaaaa gatggagcaa ttggaggaac cttatatctt gaagaaaaag
    18421 aaatagcacc tattggcgta ggtactatac taaaaataat tagaaaataa aatttaaaac
    18481 gaatctaatc atattataag taaattatag ataaaagtca atttaactaa tcaagttaac
    18541 ttttaatgtg ataaaaaagg ggaaatgaaa atgagaaaag tggctttagt cgttggagga
    18601 ggaagaagtt taggtgaata tttatctaaa catttagcta aagtaggtta tgatgttgct
    18661 attgcagatt taaatgaaga caacgcaaaa aaagtagctg aagaaataaa aactgaattt
    18721 ggttgtaaat ctgtaggata tagttgtaat gccatagatg aaagacaggt tataagtact
    18781 gtcaaagcta ttgaaaatga ttttgataga atagatttat tagtgtacaa tgcaggagtg
    18841 gctgtaagta aaaaaataac tgattttgga tacaaagatt ttaaatttat tgtagacgta
    18901 aatttaaatg ggtactttct atttgcaaga gaagtatcta aagtcatgat agcaaaaggt
    18961 attgagggta gaataataca gataaattca aaatcaggta gagtgggaag taagcataat
    19021 tcaggatatt cagcaggtaa atttgctgga gtaggactta ctcaaagttt agcgctagat
    19081 ttggcagaac ataagataac tgtaaatgca ctgatgctcg gtaatttact tgattctgat
    19141 atgtttgaaa gtttaattcc tcaatatgca gtaaaattgg gaatacctga atcagaagtc
    19201 aagcaagttt atatagataa agtaccttta aaaagagggt gtagatttat agatgttgca
    19261 aatatgctta cattttatgc ttcaaaggaa gctgaatact gcactggtca atctataaat
    19321 attactggtg gacaagttat gtaaggtttt atggagtgtc tcaagatgaa ctctttagtt
    19381 catgaggcac ttttttgtat aaaattaaat atattttcag catttaaaca atgaaaaaga
    19441 ggtttatctt tattttgaga tagcctcatt tttgtaatta aaaaacatag tgtttaagta
    19501 aacatataag attgttagtt ttttaaaaga gaatttttta ataatattat atttatgaag
    19561 tatttttaaa cttaatttga taaaatatag ctatgtttta tatactgtag ttttgagcat
    19621 agaaagttta ataaattata gtatatatgt taatttttaa taaggtaaga aagtagaaag
    19681 aaggatattt tatggatata ataaaaaaat taagtcatgg gtgtacatta gattgtcatg
    19741 actgttgtaa attcaatgtt tatactaaag gaaataatgt agtaaaaatt gaaggagata
    19801 aaaatcatcc ttatacaaaa ggttttatat gcaaaaaagg gatggcccat ttagatagat
    19861 taaatcataa agatagaata aagactccta tgcttaaagt tgatggagta tggaaagaga
    19921 tttcttttga taaagctata gaaataatgg cagaaaagct tacatactat aaagaaaagt
    19981 atacatcaaa gtctgtcatg cattatgacc aatatggaag tgggtctgtg ctaaaataca
    20041 taggagatat attttttaat ttttatggag gtgttagtag acataagggg ggtccctgtt
    20101 ggagtgcagg gatgcatgct caaaaatatg attttggagt agctaagagt catgctatag
    20161 aagatatgtt aaatagtaaa agtatatttg tgtggggaaa aaatccagca tatactacaa
    20221 ttcatactat gcaaataatt aaaaaagcta aagaaaaagg gataaagata gtagtgattg
    20281 accccatata tacaaaaaca gctcaaatag cagataagta tgtacaagta aatcctggaa
    20341 cagatggagc tttagctata gctatggcaa aaattatagt agaagataaa ttatatgatg
    20401 aagaatatat aaactcgtat gttattggtt ttgaagaata taaaaaatat ttatcttcat
    20461 tggaattaag ttttttaatt gatgaatgtg gtgttaaaga gaatgatata agagagttag
    20521 tggatttata tactaacaaa tattcaagta taaatgttgg atatgggctt caaaaatata
    20581 aaaatggtgg aaacacaata agagcaatag atgctttagg tgctataact ggtcaaatag
    20641 gatttagtgg tggaggggta aactatgcta ataaagttta tccgagtgtt ataaattctg
    20701 acccttacaa tagccaatca tatggtgaag atagggagtt ttatgtaagt aatatcagta
    20761 aatttataga agaatcttta aaaaatacat caaataaagt aaattatgca tcagatgaac
    20821 ttgatatggc attacataaa gtaaattaca tatcagatga acttgataca acatcaaata
    20881 aagtaaatta tgtatcagat gaacttgata taacatcaaa taaagtaaat tatgtatcag
    20941 atgaacttga tatgacatca aataaagtaa attatgtatt agatgaactt gatataacat
    21001 caaataaaat agattatata tcaaatgaat tgtataattt atctaataag tcaatcaaag
    21061 ataatattcc tataaagatg gctgtgataa ctaaaagtaa tatgctgaat caactgccag
    21121 atttagtaga attggagagg gtgttttcaa aaattgaatt taaagtatgt tttgatatgt
    21181 ttataactga tacagctact ttatgtgaca tgtttatacc atgtacaaat acactagaga
    21241 gtgaagatat aatttatagt tcaatgacca atccatatat aacctataat gaaagagctg
    21301 taaagccagc acataaattg atggatgaat attacttctt tatggagcta gctaaaaaga
    21361 tgggattaaa tgattatcct tttgtagaaa agagaactta tttagaaaaa gtgattgaac
    21421 cacttaagcg ttttgacaaa aatctggata tagaaaaact taaaaacaat tactttacta
    21481 tacataaccc tgttgcttgg gaagataaga agtttgagac tccatctgga aaatacgagc
    21541 tttattcaga aagtataaaa aacttgggaa taagtccaac acctgtatat ataagcaata
    21601 aatataaaga aatagaagat aaaaatattt cttttagatt attaactaat catcatgcag
    21661 atactttatt tagtcagcat tttatggaca aaaaatctat agctcaagca tatataaatc
    21721 ggagaatggc taaaaaagta ggtattgagg acaaagatat tgtgatattg aggtcaaaaa
    21781 aagcaaaaat aaatgtacaa ataaatatag atgatggagt aggtaattat atagtaaaaa
    21841 tgtatgttgg ttggtggaag aaacatggaa atcctaatag tttaacagac actggtatat
    21901 cagattttgg gggtcaggta acatataatg aaagtatggt agaaataata cgacaaaact
    21961 agttaaattt cattgaagga ataaaaatat aattatagaa ttgattaaaa gttactattt
    22021 ttacaaaact attgaaggag ggatttttga tggtaaatta ttccttagaa cataagaatc
    22081 tatacataga atttatgtgc tcagaactag accaccatgt agccaatgaa ataagggaag
    22141 aaatagataa cttattaagt gtaaatcaag taaaaaatgt tgtatttaat tttgaaaata
    22201 ttaattttat ggattcttca ggtataggcg tcatcatagg tagatataaa aagatttcta
    22261 atgaaggggg acgagtttca gtaattaata taagttcacg agttaagaag atttttgatt
    22321 tatctgggtt aaataaaatt attggcattt atgatacgta tgaagaagct ctaagttctt
    22381 tgtagggagg agtatgtgat gaataatatt atggaagtta aattttcggc aatatctgaa
    22441 aatgaaagtt tagcaagagt tatagtagca tcctttgcag ctaagttaga cccaacagta
    22501 gatgagttag tagatataaa aactgctgtc tctgaggctg ttacgaatgc tataatacat
    22561 ggatatgaag aagacagttc taagtttgtt ttcttaagat gtgagataga aggaaatact
    22621 ataaagatta tagttgaaga tgaagggtat ggaatagaaa atgtggagaa ggcaatggaa
    22681 ccactttata catcaaaacc agaacttgat agatcaggaa tgggattcac agtaatgaaa
    22741 agctttatgg acgatgtaga ggtaagttct gtaaaagata atgggactag aattgaaatg
    22801 actaaaaaaa taaatgtgcc taaataaagt ccaaaagtga ggttttatta tggaagtaac
    22861 tgttgccaga gaagaaaaaa aacctcttct tagtcatgaa gagactctag aacttattga
    22921 aaaagttcaa aatggagatg aagaggcaaa agaaatatta atatcaagca atttaggtct
    22981 agttaggagc gttgtatcta gatttttaaa tataggatat gatagagaag atttatttca
    23041 gttaggttct ataggcttaa taaaatcaat atataaattt gacccaaaat ttaatgtaaa
    23101 attctctact tatgcagtac caatgatttt aggtgaaata aagagatact taagagatga
    23161 tggtatgata aaagtatcaa ggtcattaaa gcaaatagct gtaaaggcaa agatggaaag
    23221 tgaagcttta actaaaaaat taggtagaga gccgagtata gaagaattag ctaaagcaat
    23281 agatgtagaa aaagaagatt tagtaatggc tatggaagct aatttcaatg ttgagtattt
    23341 gcaaggagtt attcatgaag aggaaggttc tccaatatgc cttattgata aaataagcat
    23401 gaaaggagaa agtgaagaag aaaaagtagt ggataatata ttgttaaaag acatacttgg
    23461 aagactggat aagagagagc gtcaaataat agttctcaga tactttgaag acatgactca
    23521 aagtgaaata ggagagatgc tcaatatatc acaagttcaa gtatctagga tagagaagaa
    23581 agtattgtct aagttaaaag aatatatatc gtgataatac ttatgatatg tagaataaca
    23641 aaatataata aatatattac taaaataaaa tgtaataact ttttatttta agtttttaaa
    23701 ttattaaaag atattaaaat aataaaaagc attaaaaaag taaaaatata aaaacaaggt
    23761 gtctttatgg aaaaatagat tttttcttta ttaggcatct tttttatttt ttgaccaaac
    23821 tttagatagt caaaataaat aataggttta tttttttaaa attaacaaat aatactttag
    23881 agaggtgttt agtatggata aaaattataa aaaatatgta gaccaaataa gcccaaaacc
    23941 tacatattta aaaaactata cacttgcatt tattgtaggt ggaattattt gtatgatagg
    24001 gcaagcaata aatgatttgt atatgaaagt tggaggtctt gataaactag gtgctagttc
    24061 tgcaacatca ataacattaa tatttatagg ggcattttta actggattag gagtctacga
    24121 cctaatagga aaaagagctg gagctggttc tataattcca ataacaggtt ttgctaactc
    24181 aatagtatct ccagcaatgg agtataaaag agaaggctat gtattaggtg taggtgcaaa
    24241 cttatttaaa attgcaggac cagttttagt ttatggtata ggttcttcta ttttatgtgg
    24301 aattatttat tatattttta agatgtttta gatgattttt atttaattta aattttatta
    24361 attatgaaga aggtataaaa tctgaaaata gctactaaag taactaattg aaaaataatt
    24421 gtgcagattt aattaatatt aaaaatttga atagtaaaat gcaaaattaa aaaggtgtga
    24481 aaatatgaaa aataaaagaa ttggaaaaag aacagtcaag ttagaaaata aaccaaccat
    24541 aatatcaact ggaactatag taggtcctaa ggaaggtgaa ggaccactaa aggactattt
    24601 tgatatgatt atgactgacg atttatatgg tgaaaagact tgggaattag ctgaaagtaa
    24661 aatggtagag actgcatctc aacaagctat ccaaaaggca ggtaaaaaat tgtcagatgt
    24721 aaattatatg ctaggtggcg atttaataaa tcaaatagtt ccagcatcat ttgcagcaag
    24781 agaactggct attccatttt taggtatata tggagcttgt tctacaatgg cagaaggtct
    24841 ttgtataggt tctatgctta tagatggtgg ttttgctgat ttagtactgt caggtacatc
    24901 aagtcactat tgtacagcag aaagacaatt tagattccca cttgaacttg gaaatcaaaa
    24961 accaatgaca gctcagtgga cagttacagg agcaggaagt gtattattag ctcctaatgg
    25021 agacggtcca aaagtaaagt atgttactgt tggtaaagta atagatgagg gtattgatga
    25081 tggtaataat atgggaccag ctatggctcc agcagcaata gatactatct actcttactt
    25141 tgaagatact aatgatgacc caaatagctt tgatataatt gctactggag atttaggtac
    25201 tcttggaaaa cagatagcag aagactttct aaaagaaaag ggtgtagata tatcaaaagt
    25261 ttatactgac tgtggtatag agatgtttaa ccttaaagaa caagatgtcc actgtggtgg
    25321 aagtggatgt gggtgttcag ctacagtgtt tgcaggatat atatatgata agttgagaaa
    25381 aaaagaattt aacaaagtaa tgcttgtatc aacaggagca ctactttcac cgacaagtac
    25441 actacaaaag cagacaatac catgtgttgc acatgcagta gtaatagtaa atgagtagga
    25501 agtgatataa tatgttaatg gactatgtaa gagtatttat tgtaggtgga attatatgtt
    25561 taatagccca agtaatgatg gattatttca aaatgcaaac tccatatgtt atggtaacgt
    25621 atgtaacaac tggtgtagtt ttaacttttc taggacttta tgagccatta gttaagtttg
    25681 cagcagcagg tgcaacagtt cctataatag gatttggata ttcacttgct acaggagtaa
    25741 taaaatcaat tcaaagtgat ggttttttag gtattttttt aggaggtaca actgctacag
    25801 ctggtggaat agcagcagct atattctttg gatatattat gtcagtagta tttactccaa
    25861 aagcaaaacc ataaatcttt attaaaagaa taataagttt ttgaaataat aactttttat
    25921 aaagcaggtg gatatctttt agattccacc tattttttat ataaagaagc gtttacataa
    25981 ttgaagttga caattattta caacatagtg tagtattata ttatcaaaat aaaatgaagt
    26041 gaagtagggg ataaagatgg taactattaa agatattgca aggaatctag gtatcagtta
    26101 ttcaactgtt tctaggtgct taaacaataa tcctaatgta tctgaaaaaa caaaaaagaa
    26161 agtggtagaa gaggcaaata gactagggtt ccattttaat gtaaatgcaa gaaatttagc
    26221 aaaaaaagaa actaacagga taggggtaat cttttcaaat aattttaatc atcaagatac
    26281 tagaaagttt tttagtgata taatggatag ttctataaat tctattgaga ctaataaata
    26341 cgattttata atacagccta ataataacat tagtggtgat agtaatgtat ataaaatggt
    26401 aaatggacaa atggttgacg gattagtaat tgtgtctcaa tcaataaaaa aagaagagta
    26461 tgatttttta aaagacaata attttccaca tgtatttata tattttaaac catcttttat
    26521 aggagaagta gataatttct tttgggacga taacgtgtat ggaggttata tagctaccaa
    26581 gcatttaatt gaccatggtc ataaagatat aataacagta acttcagatg ataagagttc
    26641 aaaaatgcat gaagatagaa ccaagggata tttgaatgct atggaagaag caaatttaaa
    26701 gacagaagtc attaaatcta aaatggattt tgaatctcaa gttgaatttt tagaaaaata
    26761 cattgataaa ataaaaaaag cttcagctat atttgtacaa caagatgtac cagcgatttc
    26821 tataatacaa gagttaaaaa ctacttatgg tataaatgta ccagaagata tttccataat
    26881 agggtataat aatattgaat taatatcata ttttaggtcg catttatcaa caatagatga
    26941 cccaagagag caagttataa aaaatggagt agatagttta gtaaatataa taaataaaaa
    27001 gagtacagaa cacattccga gaaaattata tccaaggtta ataatacgaa atagcgttaa
    27061 aagaataaag tagtaagctt ttaagtatca aacgtttatt aaaaatatta tagattatgt
    27121 gaagtttttt aacaaataat ataaataaaa attaaaagga gtaggtgtat ataagtacat
    27181 tgtactcctt tatttattga gatagttttt ataatatgaa tgaataattt tattcaatta
    27241 tttgaaaatc cgttatttta acaatataaa ttaataaatt atttgtattt tatatcatga
    27301 atctataata tttttttgat tcataactaa aagta
//
LOCUS       DAEOSP010000032        27068 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.032, whole genome shotgun sequence.
ACCESSION   DAEOSP010000032 DAEOSP010000000
VERSION     DAEOSP010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27068
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.032"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     regulatory      218..308
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            716..1033
                     /gene="flgB"
                     /locus_tag="KRH54_001112"
     CDS             716..1033
                     /gene="flgB"
                     /locus_tag="KRH54_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBG8578781.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            1041..1445
                     /gene="flgC"
                     /locus_tag="KRH54_001113"
     CDS             1041..1445
                     /gene="flgC"
                     /locus_tag="KRH54_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBG8578782.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            1455..1772
                     /gene="fliE"
                     /locus_tag="KRH54_001114"
     CDS             1455..1772
                     /gene="fliE"
                     /locus_tag="KRH54_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBG8578783.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1797..3347
                     /gene="fliF"
                     /locus_tag="KRH54_001115"
     CDS             1797..3347
                     /gene="fliF"
                     /locus_tag="KRH54_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBG8578784.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            3360..4430
                     /gene="fliG"
                     /locus_tag="KRH54_001116"
     CDS             3360..4430
                     /gene="fliG"
                     /locus_tag="KRH54_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBG8578785.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            4423..5154
                     /locus_tag="KRH54_001117"
     CDS             4423..5154
                     /locus_tag="KRH54_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBG8578786.1"
                     /translation="MNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKINAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQANLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            5158..6477
                     /gene="fliI"
                     /locus_tag="KRH54_001118"
     CDS             5158..6477
                     /gene="fliI"
                     /locus_tag="KRH54_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBG8578787.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRVFE"
     gene            6508..6954
                     /gene="fliJ"
                     /locus_tag="KRH54_001119"
     CDS             6508..6954
                     /gene="fliJ"
                     /locus_tag="KRH54_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBG8578788.1"
                     /translation="MELDKNFKFRLQKVLDLKVKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDSIQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEENLKEQNTNDEFASMSYLRNRQVL"
     gene            6983..8188
                     /locus_tag="KRH54_001120"
     CDS             6983..8188
                     /locus_tag="KRH54_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBG8578789.1"
                     /translation="MKIQADSYNIIKDIGDRKSSPKNKNIKNNKFEDNLYKATDHKKV
                     KDRREVSKQNISSDKPENSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     DNYKKDKDINTILNNIEKLSISDELKQELKLNKNILNKIANQNSELNKANINNSNDIE
                     NLDIVKLRELLEDNANKDIEVITKTTENDTKNIDLYSFDSNRMDNLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKNVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKKNTNDKHNNVVIEDDIKENTI
                     DDDRVDLLA"
     gene            8206..8814
                     /locus_tag="KRH54_001121"
     CDS             8206..8814
                     /locus_tag="KRH54_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBG8578790.1"
                     /translation="MYGIATALAGTKGNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDVLLALSNSTLINSALSMATSLIGKEVEVSGEEKEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDENSGEENDSSEHESPEEA"
     gene            8841..9824
                     /locus_tag="KRH54_001122"
     CDS             8841..9824
                     /locus_tag="KRH54_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBG8578791.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMVQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9837..10040
                     /locus_tag="KRH54_001123"
     CDS             9837..10040
                     /locus_tag="KRH54_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBG8578792.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            10040..10858
                     /locus_tag="KRH54_001124"
     CDS             10040..10858
                     /locus_tag="KRH54_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBG8578793.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMGIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10848..11543
                     /locus_tag="KRH54_001125"
     CDS             10848..11543
                     /locus_tag="KRH54_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBG8578794.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSIFSTGSSTSEKSPIDSVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKVDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            11555..11998
                     /locus_tag="KRH54_001126"
     CDS             11555..11998
                     /locus_tag="KRH54_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBG8578795.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQVDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            12013..12387
                     /locus_tag="KRH54_001127"
     CDS             12013..12387
                     /locus_tag="KRH54_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HBG8578796.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYVKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILKIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            12388..13053
                     /gene="fliP"
                     /locus_tag="KRH54_001128"
     CDS             12388..13053
                     /gene="fliP"
                     /locus_tag="KRH54_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBG8578797.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLTQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            13066..13335
                     /gene="fliQ"
                     /locus_tag="KRH54_001129"
     CDS             13066..13335
                     /gene="fliQ"
                     /locus_tag="KRH54_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBG8578798.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMISLITK"
     gene            13347..15161
                     /locus_tag="KRH54_001130"
     CDS             13347..15161
                     /locus_tag="KRH54_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBG8578799.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKPFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPVSGFKNMFSMRSLSTLI
                     KDIAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIAIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEVKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            15170..17245
                     /gene="flhA"
                     /locus_tag="KRH54_001131"
     CDS             15170..17245
                     /gene="flhA"
                     /locus_tag="KRH54_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBG8578800.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMIIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTVGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELISNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMSNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            17220..18236
                     /gene="flhF"
                     /locus_tag="KRH54_001132"
     CDS             17220..18236
                     /gene="flhF"
                     /locus_tag="KRH54_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBG8578801.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENIEQKDLKREIEQLKLIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQVINGKIALVGPQGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            18233..19111
                     /locus_tag="KRH54_001133"
     CDS             18233..19111
                     /locus_tag="KRH54_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBG8578802.1"
                     /translation="MIDQAQILRKMAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            19125..19826
                     /locus_tag="KRH54_001134"
     CDS             19125..19826
                     /locus_tag="KRH54_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBG8578803.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19842..20264
                     /locus_tag="KRH54_001135"
     CDS             19842..20264
                     /locus_tag="KRH54_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG8578804.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            20249..20434
                     /locus_tag="KRH54_001136"
     CDS             20249..20434
                     /locus_tag="KRH54_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBG8578805.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            20475..21248
                     /gene="flgG"
                     /locus_tag="KRH54_001137"
     CDS             20475..21248
                     /gene="flgG"
                     /locus_tag="KRH54_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG8578806.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            21269..22030
                     /gene="flgG"
                     /locus_tag="KRH54_001138"
     CDS             21269..22030
                     /gene="flgG"
                     /locus_tag="KRH54_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBG8578807.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            22053..22934
                     /locus_tag="KRH54_001139"
     CDS             22053..22934
                     /locus_tag="KRH54_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG8578808.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22937..23311
                     /locus_tag="KRH54_001140"
     CDS             22937..23311
                     /locus_tag="KRH54_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBG8578809.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            23343..24746
                     /locus_tag="KRH54_001141"
     CDS             23343..24746
                     /locus_tag="KRH54_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG8578810.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLYMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            24862..26799
                     /gene="htpG"
                     /locus_tag="KRH54_001142"
     CDS             24862..26799
                     /gene="htpG"
                     /locus_tag="KRH54_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBG8578811.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
BASE COUNT        11211 a         2847 c         5020 g         7990 t
ORIGIN      
        1 aataaaattg aaatatatgg aaaaatatag aattaagatg agatttgtct tatattttta
       61 taaattaaaa atattttcaa agagtttatt ataaaattgt aaactatagt gtttataatc
      121 tttgtaatta ttggtaatta aatttaaaaa ataaaatatt taattataca caaaaaagat
      181 atttaaaaaa tttgttattt attgtatact taagttaaaa ctaaataggc aaatctagag
      241 aaatctagtg acgcaaagct atagggacta aggtttatat acataaacta tgtcagccag
      301 ttgccaaaaa gagtcctagg tgtattgtat acctaagaaa agtctataat gactgccttt
      361 ttggcagtca ttttgttatg gaaaaatcac tttatttcag tagaaaatac acataataac
      421 atattttacc aattataaca taaaataaag aaaaattatc tcaaaaagtt actattttac
      481 aaaaaatgac ttctttattt atttaaatta atcgcatttc cattacatta atacaataat
      541 gtatcaattt agtaagaaaa ctaatatttt tttaatgaag tcccatgaaa atataaaaaa
      601 attttaatat gtatttaaat atttcttcga aaagttgctc tttttgtaat atagcaactt
      661 catttttttt attaataaac aaaatagata aaatatattt tgaggagggg tgagtgtgaa
      721 tacatatgac attataaaaa tgggattaga tgcaactaat ctaagaagtc agacaatagc
      781 aaataatata gcaaatataa atactgaagg atataaaaga cattatataa cttttgaaga
      841 aacactaaat gatgctatgc ctaaaataga agaaaaaaca gacaattcta aaggtttaag
      901 agtagatggt aataatgtag acatagaaga agaaaaagta aatcaagcaa tggcaacatt
      961 acaatataat gctttgataa gttttgcaaa tggaaaaata gcaatgacca aatcaataat
     1021 aagtgggagg taattagaag atgagtgtgt tcagtggaat gagaatatct gctagtggat
     1081 taacagcaga aagtttgaaa atggatatca tatcatccaa tgtagctaat ataaatgcta
     1141 caagaggtcc aaatggagaa gcatatagga gaaaagttgt ttcatttgaa gaaaactatg
     1201 ataataaatt aggtatgcta ggagtaaaag ttacatctgt tgaagatgat aaatcacctc
     1261 ttaaagaagt atatgaccca tctcatccag atgcaaagcc aaatggatat gtagaatatc
     1321 caaatgtaaa tatattggta gaaatgacag atttaataac agcttcacga gcatatgatt
     1381 ctaacgtaga tacattgaat gctcagaagt ctatgatatc aaaagctttg gaaataggaa
     1441 agtagggata aattatgcca attacacaaa ttaacaattt aatgcaaggt gcaaagttag
     1501 ataatttgat tggagaagca aagccattaa ctcttaataa taaagaagaa aaaaagaatt
     1561 ttgaagatgt aataatagat actatagata aagttaattt tcaacaagta acagcagatg
     1621 atatgaaaga aaaatttata aaaggcgaag atgtttctat gcatgaagtt atgttaatag
     1681 gtcaagaagc acaactatct atgcaatatt taatagaagt tagaaataag attcatgatg
     1741 catatcaaga aatgaataag gtgcaaattt agccttttga acaggagtaa taatcaatga
     1801 taaccagttt taaaaataag attaaggagc aagttgctaa atctaggaca aagataagtt
     1861 ctttaaaaaa gggaactaaa atagcattag gtataggggt tattgctata tgtttagcgc
     1921 tagtatttac agcaatatct gcgagcaaaa ataagtatgc agtcttattt tcagaactag
     1981 atgctcaaga tgctgcagat atatcagcag agctagagtc taaaaagata gatactaagg
     2041 taaaaggaag tacgatttat gtacctagaa cagaagttga taagttaagg ctggagttag
     2101 cgccaagtct tacaggtggt tctaaaggat ttgcactaat ggatgatagt tcatcaatag
     2161 ggcttactga tgaagaattt cagattaaga aacaaagaat gatacaaggt gaaatagaaa
     2221 aaacaataaa gagttttcca caagtggaag acactagagt acatataaca tttggagagt
     2281 catcagtatt tgagaaggag gctgttccag ggagtgcagc agtttatttg atgctaaaac
     2341 cagggacgac tttagaaaaa aaccaaataa agtctgttat ggcacttata tcaggaagcg
     2401 ttacaaatat accagagaaa aatgttcagg ttattgataa caaaatgaat ttattatctg
     2461 aaggtatttt ggaggatgaa aatgttgatg aaaataatca accaataaat agtggcatta
     2521 gtgttgataa aaataaggca tctgaacaag cattgaataa acaactagaa aagtctattt
     2581 tagaaatgct agaaccgata tttggtaagg gaaaggtgaa agctacagta aatgctgatt
     2641 taagttttga tacagctgaa aaaactgaga taaaaattga ccctgataaa gttgcaataa
     2701 aggagacaag gtcgaaaaat tcatctaaat ctgctgcaaa taatgttcaa ggtgtagatg
     2761 ctaacatgaa taaccaagga aataacaatg gtactaatac agaagatagt ttagatgaga
     2821 gttttgagta tgaaacaggt aagattgaaa ctcacactat agttgcgcct ggagaactta
     2881 atagattgac agcttctgtt gcaatagatg gaaaaatagc taaaggtgtt caagctgatg
     2941 tagaggatat tgttaataat gctataggta tggatggagc gaggggagat actcttagcg
     3001 ttgtttcaat ggtatttgac ccagaaggta agaaagaggc taaacaagag ctggaagaga
     3061 ttaaaaaaga aggatttaaa aagaacataa taaaagcagt tattggagta gtagcagtaa
     3121 tagcagttgg agctattgga tacttaatat ataagaaaag aagtgaacga gaagatatgg
     3181 attatgaaga tgaagattat atttctaaat ctgattctgt tgataggaag gataatcctc
     3241 tattaggaga tgatccagaa ctgacattag aggaagctgt taaaatgtat gcagaagaaa
     3301 agccagacca agtaacagag ataataaaga cttggttaaa tgagtaaggg ggatagaaag
     3361 tgtccacgga aattacaaat gttaatgcgt ttgacttaag tgatataaat gaaagtgata
     3421 agaatgagaa ggtagaaggt gagggtttaa aaaaagctgc tgtattatta atgacattag
     3481 gcccaaatgt gtctagtgat ataataaaaa gcttgcctga taaacaagtt caaaaaatag
     3541 gtttggaaat tgcaaatatt tcttcaatag cttcaaaaga aagaagagaa atcttaaaag
     3601 agtttgtaga aataaatagg gcaaaagatt atgttataga aggtgggctt gaatatgcaa
     3661 gagagttatt aagtaatgct ttaggaaatg ttaaagcaaa caaattactt gaaggtatat
     3721 caataaatac aagtacaaag ccttttaaac atattataaa actagatgta aagaagatat
     3781 tagaaatact taggaatgaa agtgcacaaa ctatagccgt tttacttgca catatggaac
     3841 cagaaaatgg agcagaagtt ttatcatact tagattttga tttacaaaat gaagtagcta
     3901 tgagaataag ctcaatgggt tcaatatctc cagaagtaat aaaggtaatt gaaagttcat
     3961 tagaagataa attacattca ataagtggtg atgattttaa tgattatgga ggggtgtcta
     4021 ccttagttca aatattgatt aattctgata gaaaaacaga aaagaatatt ttgggaacta
     4081 ttgaagaaca aaacccggat ttagcagctc aaattaaatc tcatatattt gtctttgaag
     4141 atattgtcaa gttagatgat ataagcatac aaaaagtgtt aaaagaagtg actatgaaag
     4201 atttagtata tgccttaaaa ggtgtagatg atgagatttc agatgctata tatagaaatc
     4261 aatcttctag agcagcagat gcacttaaag aagaaatgga aatggttggt gttataaagg
     4321 ttacacaagt tgaggcttct caacaaaaga tagctaatat cgtaagaaaa cttgaaagag
     4381 aaggtgccat aactattgta aggaattttg gtggtgactt tgttgaataa taagattatt
     4441 aaaagtaaaa aagcattata taaaggagtg ctagatttaa ccataataga taaagttaga
     4501 gtgggagaat caaatgtaaa taaagaaaat gattgtagat ttagggatga ggtaataaaa
     4561 gaagctatag aatctgctga gataaaaaag aatgagatat tagaagaagc aaaaataaat
     4621 gcacaaaata tagaaaagca agcatatgaa aaagggtata gtcaaggtca aaaaaatgga
     4681 tatgaggatg gatataaaga gtcatatgat gaatataccc aaaaagctaa agatgaagca
     4741 gattttataa aaaatcaggc aaatttaatc ttattacaag ctaatgagaa gatgactgag
     4801 tacataaaag aaaaaaataa agagattgta agacttgcta ttaatatggc cagttctgtt
     4861 ttaaaaaagc atttcgaaga tgaagaatct atgagtgatc tgttaaagca aattattcaa
     4921 gattatgaag gaagtagttc atttatagta agatgtaatt ctttctataa agataatata
     4981 gagaaagagt ttgaattatt caaagaggaa acatcactaa aatcaagtgt atttgttatt
     5041 gcagatgata gtatagataa aggcaatgct attatccaaa aggaaaatgg aagaataata
     5101 gttggtattg attgtgctat ggaaaaacta aaagaagaat tgcttggtga ataaagtatg
     5161 tataaaactg atatatctat agattttgac aaaataaata agaaaataga aaatttagaa
     5221 ctcactatat cagaaggtag agtaaagaag ataataggac ttactgtaga agttgaagga
     5281 ataaaagctt ttgttgggga gttatgcgtt atatacaacc aagttaataa accagtgaat
     5341 tgtgaagtag taggttttaa agataaagaa gtcatattaa tggcgttggg agaattaaca
     5401 ttgatagcac ctggttgtaa agttatttct aaggggatac ctttaagtgt tatgtgtagt
     5461 gataatttac taggtaaagt acttgatggt cttggaaatc ctatagacaa ctcagatgct
     5521 gttttaggag atagatacaa cctgaacaat gaacctcctg atccaatgaa gagaaaaaag
     5581 atacgaaata taatggaaac aggtgtaaga gcaatagatg catttactac ttgtggtgaa
     5641 ggacagagaa tcggcatatt tgcaggaagt ggagtaggta agagtacgac tctaggtatg
     5701 attgctagga atgcaaaagc agatgtaaat gtaattgctc ttattggaga aagaggaaga
     5761 gaagtactag attttattga taaagattta ggtgaagagg gaatgaaaaa atctgttgtt
     5821 gtgtgtgcaa catcagataa agcccctcta gttaggttaa aaggtgctct tactgctact
     5881 gcaatagcag aatattttag agaccaaggc aaaaaagtaa tacttatgat ggattcagta
     5941 actagatttg ctatggcaca aagagaagtt ggtgtagcaa taggagagcc accagctcaa
     6001 aaaggatata caccatctgt atttgcaatg ttgccaaaac ttatggagag aacagggaca
     6061 tcagataaag gttctataac tgcattttat acagtattag ttgatggaga tgactttaat
     6121 gagccaatag cagatacaac aagagggata cttgatggtc atatagtttt atctagagac
     6181 ttggcaaata agaatcacta tccttctata gatgtattaa acagtttaag taggcttatg
     6241 aatgaaattg cttcaaaaga agacataaaa atagcgtctt ttgcaagaga catgcttgct
     6301 gagtatagag aagcagaaga tttgataaat ataggtgcat atgcttcagg tacaaataaa
     6361 aaaatagatg aagcaatcta ttatcatgag catattatta attttttaaa acaagggata
     6421 aatgaaaaga gttcttttaa tgaaactata agtagtttga gaagagtttt tgaataaaca
     6481 attaaaaaaa tatttgaata ggttgtgatg gaattggata aaaactttaa gtttagatta
     6541 cagaaagttt tagacttaaa agtgaaagat gaagaagaga taaaaatgga atttgctaag
     6601 attcaacaaa aaaaaataga tatagagact aatttagaaa acttagaaag taattatagc
     6661 aaatattcta taagtaaaaa taacgatagt attcaaaatc aaaaaataac tattaactat
     6721 ttactagctt taaacaattc tataatggat ttatcagaag agttggataa gtcaacaaat
     6781 gaacttgaaa aagctagaaa gcagcttata agcaaacaga tagagagaaa atcattagaa
     6841 aaattaaaag aaaaaaagta tggacagtat tataaagaag aaaatttaaa agaacaaaac
     6901 actaatgatg agtttgcaag tatgagttat ttaaggaata gacaagttct ataagaatag
     6961 taggaagtga ggatattatt gtatgaagat acaagcagat tcttataata ttattaaaga
     7021 tattggggat agaaaaagtt cacctaagaa caaaaatata aaaaataata aatttgaaga
     7081 taatctatat aaggcaacag accataaaaa agttaaagat agaagggaag taagtaagca
     7141 aaatattagc tcagataaac cggaaaatag tgataatgag tgtcatttag atgaagataa
     7201 aagtaatcac ttagaagatg aaaatacata tgaaaaagat tgtagtaaga ataatgaagc
     7261 tttgcagcaa ttactgaatc ttttaaagga taattataaa aaagataaag atataaacac
     7321 tatattgaat aatatagaaa aactaagtat atcagatgaa ttaaaacaag aacttaagct
     7381 taataaaaat atattaaata agattgcaaa tcaaaattca gagctaaata aagctaatat
     7441 aaataactct aatgatatag aaaatttaga catagttaaa ttaagagaac ttttggaaga
     7501 taatgctaat aaagatattg aagtaattac taaaactaca gaaaatgata ccaaaaatat
     7561 agatttgtat agttttgata gcaatagaat ggataatctt aactcaaata aacagaaaga
     7621 tgatagctct gatattttgg aaaaactagc aggggtaaat ggaaataaga gtgggaattt
     7681 tggacaagta ataaataaaa atgttgatat gaataaaatt aaaaatgata atgtcaattt
     7741 aaaacagata gaatcaaatg taatggatga ctctataaaa gctattaagc atatgaaaac
     7801 aaatgatata caagaattga caattaaatt aagaccaaaa gaattaggag atatgaatat
     7861 acaattgtta aaagatggtg aaagtatgag agctgttgtg actgttttca ataaagatgt
     7921 ctttgactct ataaataaaa acatagcaga tttaaagcaa catcttgaac ttactaatgt
     7981 aaatataaaa gatgtatcag tgcaaatgca cagcgataat acaaatacat ctgatacctt
     8041 tgataaagca tttgaacaac agaatagaca aaataaccaa gaaaacatga gtaagaagaa
     8101 tacaaatgat aagcataata atgtagtcat agaagatgac attaaagaaa atactattga
     8161 tgatgataga gtagatttac tagcttagta agggggaagt attgcatgta cggaatagca
     8221 acggctttgg caggaactaa gggaaactat aggtcaaata acttaagcca acttgaaaga
     8281 aatattaatg atattgccaa taaacaagtt agaagcaaag gaaatcagac agtaacagac
     8341 aatgggactc caattatagg acaaggtgat gatgctgata aagacttgtt tatgaagata
     8401 cttgtggcac agatgggtaa tcaagaccca atgaatccgc aagacccaac agaatatgtg
     8461 tcacaattag cacagtttgc aactttagaa aagatgtcag ctatgaatga gaagctagat
     8521 gtacttttag ccttaagtaa ttccactcta ataaactctg ctttaagtat ggcaacatca
     8581 ttaattggta aagaagtaga agtatctggg gaagagaaag aagatgaaaa cattgtagga
     8641 gtagtaaagt ccacatatat aaaagatggg gaagtacact tagaattaga agtagatgga
     8701 agtgacaaaa ctgtaggtgt taaatatgaa aaattactta aaataggaga ggtagatgaa
     8761 aattcaggag aagaaaatga ttcttcagaa cacgaatctc cagaagaagc ataatattaa
     8821 atgagaaaag aggtaaattc atgataaaag caatgtattc aggtgtaagt ggaatgaaag
     8881 caaatcaaac aaaattagat gtaataggta acaatgtagc caacgctgga actacttctt
     8941 ttaagaaaag ttcagctaga ataacagatt cattttatca aactgtacta tatgcaagtg
     9001 ctccaacagc tgcattagga ggaacaaact taggacaagt aggtgtaggt agtaagatat
     9061 caagtataaa taaagatatg gtacaaggaa atgtacaacc aactaataga ccatctgatt
     9121 taatgataga tggagatgga tatcttccag tagtaagaca aggaacagtt atgtatacga
     9181 gagatggttc ttttaattta gatacaggat ttaaagcagc tgatggtgct ttgaagaata
     9241 ttaaaggtgg aagacttgta aatggtgatg gatatatgtt acaaggtatg atttatgatg
     9301 gggaatataa agctgatact ggagaatttg ttaaaaaggg tgatgctaaa ggaaaagagc
     9361 ctattgtaat accacttcaa agtataagtc ctaaggatgg aacagttcaa aatgtactag
     9421 agtataatat atcaaaggat ggaacagtag aatttttatt gagtgatggt caaagaactc
     9481 aatttgtcta tgatatacct gatggtgcta aggtaggaga tacacctgct ggaatagata
     9541 aattaaaagg tacaatacaa aaagtgcaaa catatgcatt ccaaaatcca gcaggactag
     9601 atgcagaagg aggaaacttg tttaaaccat cagcaaactc aggagaccca caaatagcag
     9661 gagctacagg aacaataata caaggggcaa tagaagcttc caatgtagat atagcagaag
     9721 aatttacaga aatgataata gcaagtaggt ctttccaagc aaacagtaaa ataatatcga
     9781 catcagacga gatacttcaa gaaataataa atcttaagag atagtaggta catattatga
     9841 taagagtaac ggatttaaac ggaagaaaat acatattaaa ttcagacctt atagtgagga
     9901 ttgattctgt accagaaagt aggattctcc ttacaacagg agaaaaaaac cttgtaaaag
     9961 agtcggtaga tgagatagta gataaagtaa taaagtataa aagaaaaata aatgtaggtt
    10021 atattaggag cgaaaaataa tgaaaaaaac agattcactg acatttattg gactaatagt
    10081 atctacagta ttagttttag ttggtgccgc aaaaggaagt agttcagggt taaagaattt
    10141 ttttgatgtt tcatcaatat taatcacagt gcttggttca tttggagctc taatgattac
    10201 atttactata gatgatataa aacttataaa aaatgcatta caatactcat ttaagacaat
    10261 gagtgtatca aagttagatt tactagaaca atttaaaaca ctttctaaaa aagctagaaa
    10321 agaaggtctt ttatcaatag agagtgatgt aatgggaata gaagatccat ttatgaagaa
    10381 agggttagag ctgtgtattg atggattgga aatcaatgag ataaggtcca tcttagaact
    10441 agagatagat tctgttgaag atacaaataa cagatactct aaactattta aaacttgggg
    10501 aacctttgca cctgcttttg ggatgttggg aacactaata ggtttaatac aaatgttagc
    10561 tgaccttaca tctccagatt taatagcatc tggcatggga aaggcattaa ttacaacgtt
    10621 ttatggttct ttacttgcca atatcgcttt gaacccaatt gcatataata tagatgagaa
    10681 gactgaaaaa gaaatttatg taaaggaaat gatgttggaa ggtataataa gcatacaatc
    10741 tggggagagc agtatagtag ttgaggaacg tttagccaca tacctttcaa acaatgagaa
    10801 gttagaaatt atgaaatcca ataaaaatac agaaagagca atgagcaatg gcgcgtaaaa
    10861 aaaataagaa aaaggatgag ataaatccag atgcatggct ggctacttat gcagatacta
    10921 taacactgat tcttacattt ttcgtattat tgtatgcaac atcagttgta gatatggaaa
    10981 aatttaaaaa catagcagat gcgttaaaag gtcagttttc tgggacaagt atatttagta
    11041 cgggttcaag tacaagtgag aaatctccaa tagacagtgt tttagtggat aaaaatgata
    11101 aatcaaattt agaacaggat ttaaaagata aagttaataa ttctaactta caaaatagtg
    11161 taacagtcaa agatgatagt agagggattt tactagagtt agatgattca attttatttg
    11221 actctgggat ttctgagtta aaagtagata gtaaagctac tcttaataaa gtatatgaca
    11281 tgataaagat gatgaaaaat aagatagtta tagaaggcca tactgataat gtacctgtaa
    11341 aatcaagtac acatgaatcc aattgggatt tatcttctag tagagcagta agtgttgtaa
    11401 gatactttgt agaagaaaag ggcatggacc ctagactatt ttcggcaaca ggatgtggag
    11461 agtatcaacc tttggtttca aatgaaacag caaagggaag ggctgagaat agaagagtta
    11521 atatacttat aactattgac taggaggaag aggtttgttc aaaaataaaa aaattattat
    11581 aattattttg atactgatta ttttctcagc gggagcaggt ttttttatat taggagaagg
    11641 tggaaataaa caagttgaca agattgcagg tttgtttatt ccaacaggaa aaggtaaaga
    11701 tgaagtaaag accgaaaatc ttgacttaga agagatggta ttaaagctag ctgatgaagg
    11761 tgtaagatat ttaaaagtat caataactgt atcttatgat tcttcatata aagatataga
    11821 aaaaaatacg cctttaatta gggataatat tataaattgt tttatggcaa aaagggcaga
    11881 tgattttact gctgataatt taaagaacat aaaagaagaa attaaaaata cattaaatat
    11941 acaactaggt gaaaatgtaa ttcaaaatat atatttcaca aatatagtag tgcaataaaa
    12001 ggatggtaag acatgatact ttatttacta aaaatattaa tatgtatccc aataatttta
    12061 atattgatag tcttatcttt aaaaattagc caaggaactt tattaaaaaa atataatgat
    12121 aaatatgtaa aaatactaga tattgtaaat ataagtaaaa acaatagcat aataatttta
    12181 aaaataggtg aagaaggatg cattatggca tcttcttctt ctagcatgag taaaataaaa
    12241 gatttgacca aagaagaaat attaaaaata gaaaacgaca ttaaagaaag aaattttaaa
    12301 attcaagatt ttaaaatgga aagaattaaa aagaaatatg atttcagtag atatattgga
    12361 aaatttacaa aaaaggaaga taagtaaatg gaactattat caaaattacc tttggctgat
    12421 gtaccatata caagttcaat gcaattgttt ttatttctaa ctgcattaat gtttttacca
    12481 tttgtaatgt tcatgatgac aagctttgtc agaatagtta taagtttatc atttttgaaa
    12541 tcagcattag gagcacagca agcaattcca agtcaaattt tggtaggact tgcaatagta
    12601 ttgacaatat ttataatgag accagtatta aatgagataa acgaaaaggc attacaacca
    12661 tatatgaaag aagaagttac tatggaagaa gctatgaagg aagcagaagg acctattaaa
    12721 gagtttttat taacacaaac cagacagaca gatttagatt tatttgtaga acaggctggt
    12781 ttaaaagaaa agaaattaac tcgtgaaaat ataccactat ctgttgtagt tccagcattt
    12841 gctataagcg aactaaaaac tgcttttcaa ataggttttt taatatatat accatttttg
    12901 ataatagacc ttgttgtggc aagtgttttg atgtctatgg gtatgtttat gttgcctcca
    12961 gtcatgatat cattaccatt taagttatta ttatttgtaa tggtggatgg atggaacttg
    13021 atagtaaaaa cattgatatt gggatttgga taggagaaaa atagtatgag tgaatttatg
    13081 ataatgagtg ttcttaaaaa tggcatgttt acaggagcta tgattgcagc tccagtactc
    13141 attgtttctc ttgtagttgg gcttttaata agtgtgtttc aagctacaac tcaaatccag
    13201 gagcaaactt tgacttttgt accaaaacta atagctattc ttttagtagt agctattggt
    13261 ggaagctgga tgttacatac tcttattagc tttacttctg agatattcaa tatgatatct
    13321 cttataacta aataagtagg atacatatga tttttgtatt tattagaata gtagcattct
    13381 ttggaacact aagaatacta tttccagctg gtacacctgc tttatttaag tcactctttg
    13441 caatcattat atcaatattg atttcaagta ctatgcaaat tgaatatgca acaaatatag
    13501 acaacattgt attgtttacc ttatatggta taaatgagac cataactggg attatgcttg
    13561 gatatataac caacctatgt ttttatagta tcagaatggc gggttctatg atggaccaac
    13621 agatgggatt atcaatgatt aatatgtttg acccaaattc aatgactcag acaactttaa
    13681 ttgataatct tatgaactgg acagcactta tgatattttt tagcatggat ggtcatcatg
    13741 ttttaattag aggtataagg tatagttttg agttaattcc aataggaaaa ccatttgtag
    13801 ataataacat agattatata ataaacatat ttgtgcagtg ctttttaact ggattcaaaa
    13861 tagcaatacc tattgtactt tgtctgctta tggcagattt tattttaggg cttatatcaa
    13921 gaagtgtacc tcaattaaat gtaatgatag ttggtatgcc attaaaaata ttggttggta
    13981 ttgcattatt tatcatatca atacctttaa ttgtcaatca aataagtcat ttattaagtc
    14041 aaataccaaa gatgtatgag ggaacttttg cactagctcc tatgtttttt atgggttcaa
    14101 cagataaaac agaagaagct actccaaaga aaaagggaga acaaagaaag aaaggtaata
    14161 tagcaaagag tagagaatta cccgttgcaa tgactctact tgcattcacc ttattagtac
    14221 ctacattatt ttcatatgta gtagatacac ttaaatcttc gcttaattat tttttgagtc
    14281 tagattttta tatgaacata aattattcaa acctagagaa attagttata gcaggattaa
    14341 tggatttttt taagatattt ttaccaatag ctattccctt tttagtttta ggcataatag
    14401 caaacctatt tcaggtggga atactgttta ctggtgaaac tttaaaacca aatttatcaa
    14461 aattaaatcc agttagtgga ttcaaaaaca tgttttctat gagaagtttg tcaaccttaa
    14521 taaaggatat agcaattata tctattttgg cttatatagg atatacattc ttccaagata
    14581 attatttaga tatattaaag ctaggtaaca tatacctacc aactttaatg tacacagtta
    14641 aagatttagt atattcaata ttgagtaaga tttgtgtagc tatgatagct atagcagttg
    14701 cagattatgt ttatcaaaga tacagtcata aaaagcaact gagaatgaca aaacaagaag
    14761 ttaaagatga atataaaaac tcagaaggag acccagaagt aaaagctaag ataaaacaaa
    14821 aacaaagaca aatttcgtct caaaggacaa tgcaagcagt accaagtgct actgtaatag
    14881 taaccaatcc aacccactta tctatagcag tgagatatga aaaagggaaa gaccaagctc
    14941 cagtagtggt ggcaaaaggt gcagattatt tagcttttaa aataagggaa attgcaaagg
    15001 gaaatgacat ccctataata gaaaacaaac ctattgctag gttgttatat aagcaagtag
    15061 aaatagacca agaaattcca gaggacatgt atcaagcttt tgcagagata ttagtggcag
    15121 tatataaaat caaaaataga tacaaagtcc ccaaaaggta ggataagtaa tgaataagtt
    15181 taatttaaag gaaaatatgg acgtaatagt ggcatttggt gttgtaggga ttgttcttat
    15241 gataatagtg ccattaccta catttatatt agatatttta ctggcaatta acataagttt
    15301 atctgtgcta attttactaa tgacattatt tacaacaaat gtacttgatt tatctatatt
    15361 tccaacagtg ttacttgtga caacactatt tagattggga ttaaatcttt catcaactag
    15421 attgatttta acacagggat atgcaggtga agttatagag gcatttggaa gttttgttgt
    15481 aggtggaaat tacgtagtag ggataattat attcttaatt atattaattg ttcaatttgt
    15541 tgttataacc aatggttctg gaagagtatc cgaagtaaca gctagattta cattagatgc
    15601 tatgccaggt aagcagatga gtatagatgc agatttaaat gcaggagtta tagatgacaa
    15661 aactgctaga aaaagaagaa aagagcttca acaagaagca gatttttatg gttctatgga
    15721 tggagcaaat aagtttgtaa agggagatgc catagcaggt cttatagtaa cggcaataaa
    15781 cataataggt ggaattgtaa taggagctgt aatgctgaat atgactctta tgggtgcagc
    15841 acagacatat gtaagactta ctgtaggaga tggtcttgta agtcagatac ctgctctatt
    15901 aatatctaca tcagcaggta taatcgtaac aagagcttct actgatgaaa actttggtgg
    15961 attagtaggt aaacagttaa cagcaatacc aaaagcagta atgatgactg gctgtgttct
    16021 aatcgtactt ggtatgatgc ctggattacc taaaccagca tttttttcac taggtatagg
    16081 tgctatagca gcaggttact ttttaggaag agaaaagaaa gatactttag aagacttgga
    16141 gtttgaaaaa cctgatatgg taaatccaga taaaaatgaa gtagaagatg taacaaactt
    16201 aataaatgta gaatcaatag aagtagaaat tggatatggt ttaatatctt tagctgatga
    16261 atcagcagga ggagatttac ttcagagaat agcatcaata agaagacaat gtgcaataga
    16321 catgggtata gtcgtacaac ctataagaat aagagataat ttacagttaa atccaaacca
    16381 atacaatata aaaataaaag ggaatgtagt tggtgggtat gagataatgc ctaatatggt
    16441 tttatgtatg gatcctatga atcaaggatt tagtatattt ggaataaaga caattgagcc
    16501 tacttttgga atagaggcac tttggataga aagtgacaag gtagaagatg cagagttaaa
    16561 gggttataca gttgtagatt catccacaat acttataact cacttactag atataataaa
    16621 atcaaatgca cacgaattac tgggaagaca agaagttaag actattatag atgcagcaag
    16681 agaaaattat agtgctgtag tagatgaatt gataccagat ttgatgacac ttggtgaaat
    16741 tcaaaaagta cttaaaaatc tgttaagaga aaaagtaaat ataaaggata gagttacaat
    16801 acttgaaacg cttgcagacc agtctagaaa tacaaaagat atagagcttc ttactgaata
    16861 tgtaagaata gccttggcta gaagtatttg taataattta gttgatgatg ataagacaat
    16921 tgtagtggct acattgtctc cagaaactga agagttgata tctaataatt tacagaggtc
    16981 tgtaaatgga acttatccag cagttgaccc agaaaataca aataaaatat ttgaaagtat
    17041 acaagaagta atgagcaatg tatatttcaa tcataatata ccagtaatag tagtatcacc
    17101 aaagataaga gctccattta gaaaattagt tgaaattgta tatcctaatt tgactgtatt
    17161 atcattaaat gaaataccta acgatattaa aatcaaagca gaaggtgtgg tgagcatata
    17221 tgatgacgaa aaagtataca gctaacacaa ttcaggatgc tatgaatcta gccaaaatgg
    17281 aattgggtga caatattaca ttaatagata aaaaagaagt aagaaagtct ggaatacaag
    17341 gtatcttttc aaagaaagat attgaactaa ctattggatg ggaaaagaga gaaaacatag
    17401 aacaaaaaga tttaaaacga gagatagaac aattaaagtt aattattaac aatatggggt
    17461 ttgataataa gaatgataat gatatagata aaatatgtaa aaatctacta agtttagaat
    17521 tgaatgaaga aattgtagaa tctataagaa ttgatttgca agaaatgaag ttcaatggaa
    17581 tcgatacaag taaaaattta gtagaaattt taaagaaaaa gattaaaata gaaaatcaag
    17641 taataaatgg taaaattgct ctagtcggac cacaaggagt aggtaaaacg actacaatag
    17701 caaagttagc cgcaaaatta gtttttgaag aaaataaaaa agtaggagta ataactatag
    17761 atacatacag gattggtgct gtagaacagt tgaagatata tactgatata atgaatattc
    17821 cctttaaagg ggtaataagt cctgatgaaa tggaattggc acttgacgag atgaaagatt
    17881 gtgatgttgt attaatagat acaacaggta gaggctataa aaactctatg caaatactag
    17941 aaatcaaaaa tcttatagat aaagctgaaa ctgataatat acatttagtt ttgaattgta
    18001 caacaagaga aagtgataca aaagcaatta tagattcata tagaaatgtg aattttaaaa
    18061 gcttaataat tacaaagcta gatgaaacaa taacatatgg atcaatattt aacataatga
    18121 attatgcaca aaaaccaata tcatatatta caacaggaca aaatgttcct gatgatatta
    18181 taaaacctaa tgaagataag attataaggt tactattagg agttgaaagt atatgataga
    18241 ccaagctcaa attttgagaa aaatggcaat ggaaaaaaga ggacttgatg agtttatagt
    18301 agagaatgag aatgtaccta agataattac aatagcttct ggtaaaggcg gtgttggaaa
    18361 gagcaatctc gctacaaatt tgtctatatg cctgaccaaa cttaataaaa aagttcttat
    18421 attagatgca gacataggaa tgtctaatat agatataata atgggggtaa atgtaaaagg
    18481 gactataatt gatgtaatta atggcgaaaa aaatatagaa gatataattt cacagactaa
    18541 gtatggtgta aatataatat ctggaggttc tgcccttaat catatagaag actttacaga
    18601 agctcaaaga aataagttta tacatagcat agaacagatt cacgatgtgg attttataat
    18661 aattgataca ggagctggta tgagcaaaag cttattgtca tttatatact gtagtactga
    18721 atttttctta ataacaactc cagaaccgac atcattgact gacgcatata gtttgttaaa
    18781 agctataagt aattttggca taaagagaag tgcaaatata ataatcaata gggtaattga
    18841 tttagaagaa gcaaaatcta catacaaaag aataaaaatg gttgtggaca aatttctaaa
    18901 ccttaatcta gaattgtatg gatatataat agatgacaaa aaagtaagtt tatgtgtaaa
    18961 aaaacagata ccattcattt tagaatatcc tacaagtatt gcatcaaaat gtattatagc
    19021 catagcaaag agaatggtaa gtcagtcaag agaagatatt gttgataata aaagttttct
    19081 aagaaagatg tttagtatat ttaaaagcta aggaggcgta gttaatgaat agagaagaat
    19141 taataaaaga gaatatgcct cttgtaaaga gtatagcaag aaaatttttt ataccaggga
    19201 aaggttttga atatcaagac ttagtaaata gtggagtcat tggattgatt gatgcaataa
    19261 ataaatttga tgctgaaaaa ggggcaaagt tttctagtta ctcttatata aaaataaaat
    19321 ctgctatatt agatgaaatt agaaatcaaa gtccaatttc taaacataat ctaactaaag
    19381 taaataagta taatagagtg gtagaaaagt tgcaatctaa attattaaga gagccaactt
    19441 catatgaaat tgcaaaagaa ttaaaggttt cagaaaaaga acttcatgat atcgaaagta
    19501 atatagatat gttaaatatt gtatcattaa actatgtaat ctttgaagat acaaatgaaa
    19561 ctgtacaaga tgtaataagt gatagagaag aggaagctcc agaaaatata atagaagaag
    19621 aagaaaagtt agagattcta tccaaagcaa ttagtaattt aaatgaaaga gaaaaattaa
    19681 tactttcact gtactattat gaagatttaa atctaaagga gatagggaaa gtgctaggtg
    19741 tttcagaatc aagagtttca caactccaca gaaaatcaat aagaaactta agaaacaaga
    19801 taaaagaact taaatattct atatagatgg tgattgataa gatgatacaa attatattaa
    19861 tagtagtgtc tattttaata atagtaacta tagttataaa tttacataag gaatcaaaaa
    19921 ataaagataa cagatacttt gataaaatat ttgagcaaga atatgaagca aatgatagat
    19981 taataattgc agaaaaaaga agattctttt tagacaaagt acttgcagaa aaaagaatta
    20041 atagtcatat aaaagtttat gaaaattata acctaataaa ggctaatgaa gaaagaaagg
    20101 aaaacatatt tatagaatca aaaaaggaag taagacaaga aaatgagtta tctcttaaag
    20161 tgaaacatct tataagttca ggttatgatg atgtggaaat atgtaagatg ctagatattg
    20221 gaaaggggga actgttatta ataaggagtt gttacaaaat ataaggatat tattatgtga
    20281 taaaaaagta ttcttaggaa taggtattgg tatattaata tcaactttat gtataatgct
    20341 ttttagtaat aatgatatga gtaaggcaga gatagaaaaa aaggccagag cgttaggcat
    20401 gaaatataca agtgagatga aagttttaga atagatgata ttgtttagac atttacaaaa
    20461 aaggaggtca taatgtgata agaggattat atacagcagt ttctgctatg ataactaatc
    20521 aacaaaagca aaatgttgtt gtcaataacc tagctaatat ggatacaaat ggatataaaa
    20581 gtaaacaact tttgactaaa agttttgatg aattaaagtt ggagggttac aataactacg
    20641 caaatggaac taaacaaaag cagattatag gcgatataag tccaggtatt tctatggatg
    20701 aaactatcac aaattttaat caaggaccta taaaaactac tgataataaa atggatgtag
    20761 ctattcaagg taagggattt tttctagtta gtgatgcagc tggaaatcaa ttttacacta
    20821 gaaatgggaa cttcagaaca gacaatcaag gtgaccttat aacaagtgaa gggtattatg
    20881 tacttgggac gaatactgct actggtgctg taggtcatat aaatgtagga aatcaaaaat
    20941 ttgaagtggc aaaagataat acaatatcac tagacggaaa tgctatgtat aaatttaata
    21001 ttgtagattt taataactac aataacttaa aaaaagaagg agataactta tattctggag
    21061 gaggagctat gaaagtaaat aatgcactta caatacaaag tgcgatagag tcctctaatg
    21121 tagatatggt atctgaaatt aataatatga tgacaatatc aagagaatat gaggcaaatc
    21181 aaaagataat tcaggctatg gattctaaat tagcaaaaat agcaagtgag attggttcag
    21241 taagataggt aataggaggt gaatttttat gtacacaatg ttttatacta gtaaaacagc
    21301 tttatcagca aatcaaagta aactagatat aatatctaac aatatagcaa attctgattc
    21361 aacaggatat aaaaaaataa atatggaata ttctgattta gtggatgaag tactaaatag
    21421 accatcatat cctacaaatg gcaatgatat atcgactgga acaggagcta aggcaacagc
    21481 tcccatgaga aattatgaac aaggtgcatt ggctcctaca gatagtaaga gtaatttggc
    21541 aatagatgga gaaggattct ttagagtgat aagaaaagat ggtacatatg catatacaag
    21601 aaatggtgga tttaatgtag atgctttagg taagattgta gatgataatg gaaatatatt
    21661 agatgtccaa tttgatgcag gatataacta taataataca aatatagata gtacaaacct
    21721 gacagtttca agaaatggag agctattcgt agataataaa aaaataggaa aaatcaactt
    21781 atatcaacct ataggaactc aaaattttat atctgaagga gatagtttat tcgtcgcaac
    21841 tgatgcagca cagattaaac aagttgagaa agtcgatata gttcaaggct atagagagag
    21901 gtcaaatgtt tctttacaag aagaatttgt tgacttaata gccactcaaa gagcatttca
    21961 aatgaactca aagggaataa agacagcaga tgaaatgtgg caaatagcga ataatttaag
    22021 agctaaataa ttatgtaagg tgaggaatat tcatgaatga tatgaaagag tacgattttg
    22081 gcaaacctca gagttattca agagaaaatt taacttgttt aaatttttta ttagctgaat
    22141 tttgtaaaaa atataaaaac tatataattt atgaacttaa gtgtaattct agtatggttg
    22201 ttgatagtat agaccaagta aattatcaga cttttttaga tagagtaact tcttcatgcg
    22261 tagtagtaca aaatgcaatg gaacctgtta tgaaaaactt ttcttttaga atagatagaa
    22321 gtgtagctga tatgtggatt gatataactt ctggaggaac tggagttgta aaggataatg
    22381 atagagaact tacagaactt gataaaaaag tgcttttaca ccttatggat gatttagtaa
    22441 gaaatatgca cttatttgag ggttttgaaa atatacaagt attggataca catacacata
    22501 ttaatttgcc acaattatgt tctccaactg caccagtatg cgttgtagat ttaaaagtgc
    22561 taaatgataa tgagtatatt ggaagtgttt ctctttgctt tccatacaat ggtttagagg
    22621 ctctgttaga aagtatatct gttatagaat tttttgataa tgatgtagga gatgattcag
    22681 aggaatttac ccaaaaaatc tataatagtg tatctaatat agaattatca gttattgcag
    22741 agataggaaa agtatcaatt aatgttgagg atttactcaa actagaagtt ggagatgtaa
    22801 tagtaacaaa taaaaaaata aatgattata tagatatatt tgtagaaaat tcaaaatcat
    22861 acacagcaac accaggattt atatcaagta aaaaaggtgt aaaaataaat gatgctgttg
    22921 gaaaagaggt gtagttatgg acaatagtaa tcttatttca gatgaagaag caaagatgtt
    22981 attaaatgat aaagttgaaa gtgaagttga tggagatgat gagttagcct tagatgtctc
    23041 tgaagttaat cgaaataata tacaaaggat acttgacctt aagttagaat tatctgtagt
    23101 tataggaaga actaagatga ctctaagaga tattttaaat ttacataaag gttctcttat
    23161 tgaactggat actttggcag accaagatgt ggaaatacta atagataata aggttttggc
    23221 ttatggaaaa gtagtggtgg tagatttaaa ttttggtgta aagataacaa gtatagtaag
    23281 tgaagaagac atggtaaaat ctcttgttta gagataacat caattaatac ttaaggcatg
    23341 aggtggtact tattttaaac ttaaatgata taaaaaaaga tatactaaaa ctcaataaaa
    23401 aagactcatg ttctctatat aatgaatcta ttaataatat aaataataat tgtatatcaa
    23461 ctggattaaa atttttaaaa caagcaagag atttaaaccc agatgatgtt gatatattaa
    23521 atctcacagg tcttgtaaac ttactaaaat gtaattttga tgatgctgta gaaagtttct
    23581 ataagagttc actttgtgaa aaaactgctc tgtgtgaaaa gtatataaac atgcttacat
    23641 ctgaggagtt tttaattttc tttgaaaagt acaatcaact tataaggtta ataaataaag
    23701 gaatgtataa agaagctata caagggttta aacttataac tgaccaatac tgtgatttaa
    23761 tagaacctta tgagttgctt gccttactgt atgacaagga agaagaatat ataaaatttg
    23821 atgaatgttt agaagtattg aagattattg ataaggaaaa ttatttatta aatcaagaaa
    23881 ataacacatt atatatgtat aatagtgcta atatacaaac taaaagtaat ataaaagtag
    23941 atgattataa gaatgagttc ttacataaaa gtagtagtaa ccataaaatt aatgattata
    24001 atgatgtaac taaagaaaag agttcaagca aaaaccagaa aagttcagat tctaataaga
    24061 aaaaaagtat taaaaaaaat aagtacctat atatagccat aatattagga atattattag
    24121 taggacaaag tatatatagt tcttataaga tgaataattt aactgataaa atatctaaaa
    24181 ataatgaaac taaaaatgaa gagtcaatag attctaactt gactaagaaa gaaaataata
    24241 tagaagaaaa tgaagatatg agtaaggctt ctaatataaa taagagtaat aaaaacatta
    24301 ataaagagga tgatgtaaaa tctaaaaaaa atactgtaga aaatgaagat gataaagatt
    24361 ctgacttatt tacagaggat gaattgatgt ctaaggcgaa tactttaaaa tcagaaaaga
    24421 aaactaaatc ttctatacaa ctatataaga aagttgcaga tataggtgaa aataaaggga
    24481 atacatctga agcaacttat caagttgcta ttctcagtga aaagttaaaa gactataaaa
    24541 ctgctgaaga atattataaa atgtatgtag aaaattactc agaaaaagat gcttattttg
    24601 atgaatctta ttataattta ggtatgatgt actataacaa tggtgattta aaaaattcta
    24661 aattgacatt aaagaagttg gttaataagg taccaaatag tatgtataat aattcaaaag
    24721 taaaagaaat tttaaaagaa gaatagttaa attagataag aaaaataaat tattttattt
    24781 taaaataata tgattataat atattgacat accattttaa aaaatttaaa atattaatta
    24841 taaaatgagg agggataaaa aatggaattt gaaaaaggga gtatttcaat tcatacagaa
    24901 aatatttttc ctataattaa aaaatggtta tactctgaca aagacatatt tataagagag
    24961 cttataagta atggatgtga tgcagtaagt aaacataaaa gacttgtatc actaggagaa
    25021 atatcagaaa ataagtcatc agattataaa attacagtat ctgtaaataa aggagaaggt
    25081 acacttaaat ttattgacaa tggaattggt atgactgaag aagagataaa aaaatacata
    25141 aatcaagttg ctttttcagg tgctgaagat ttctttaata aatataagga taaaatggaa
    25201 gagtcaaatg atataatagg tcattttgga ctaggatttt attctgcatt tatggtgtct
    25261 aaaaaggtac aaatagatac actttcatat acagaaggag cgactcctgt aagatggata
    25321 tctgaaggtg gtacagaata cgaaatatct gaatcagatg ctagaaatga tagaggaact
    25381 actataacac tatttattga tgatgatagt aaagaatttt tagatgaatt tactgtaaga
    25441 ggaataataa ataaatactg ttctttctta ccagtagaaa tatatttaga agatgttgaa
    25501 agattagaaa gagaagctaa agaagcagaa gaaaaagcta aaaaacaaaa ggaagatgga
    25561 aaagaagaga ttgtagatgc aaaagtaatt gagcctttaa acgacactaa tcctctatgg
    25621 ttaaaatctc caaaagactg tactgatgag gagtataaag agttttatag aaaggtattc
    25681 aatgtatttg atgaaccttt attttggata catttaaatg tagattatcc tttcaattta
    25741 aagggtatac tttacttccc taaattaaaa aatgagtttg aactgactga aggaaaagta
    25801 aaattataca acaatcaagt gtttgtggct gacaatataa aagaagttat accagaattt
    25861 ttacttttat taaaaggagt tatagactgc ccagacttac cattaaatgt atcaagaagt
    25921 ttcttacaaa atgacaggga tgtaagcaaa atttctaaac atataattaa aaaagtagct
    25981 gataagttga aatctctttg taaaaatgaa agagaagaat acaataagtt ttgggatgat
    26041 atacaaatat ttattaaata tggttgctta aaagatgaaa gtttctatga gaaggtaaaa
    26101 gaatgtatat tattcaaaac tattgatgat gagtatataa cattacaaga ttatttagaa
    26161 aaatgtaagg acaaacatga aaataaggta ttttatgtaa gtgataagga acagcaatct
    26221 caatacatta aactatttaa agaatacgac ttaagtgctg ttgttttaaa ctcatcaata
    26281 gatactcatt ttatttcatt tatggaatat aaagagaatg gagttaagtt taatagaata
    26341 gatgcagacc tttctgatgt attaaaagat aaaaatgaaa ataaagatag tgaagaaaac
    26401 aaagaagaga tagctaagat agaaggtttg tttaaagaag ctgtcggaga aagagtgaag
    26461 aattattctg tagaaggatt gaaaaatgaa gacactccag ctatggtatt agtatcagaa
    26521 caatctatta gaatggcaga aatgcagtca agatttgctg gaatggattt aggaatgaat
    26581 tttgaggaag aaaaaactct tgtaataaat gagaacagtc caataatcaa aaaattggtt
    26641 tctcttaaag atgatgagga aaagaaagat aagattacgc ttatttgtaa ccagatagca
    26701 gatttagctt tactttctaa taaagaatta aagccagatg agttagattc atttgtacaa
    26761 agaagtaata agcttatgag tcttttgata gaattgtaag attgaatttg aatacattga
    26821 agcaaattta taaaaagtat tcaatatcag tgatgagaaa gaagtgtctc aaaatgaact
    26881 tttcagttca tgaggcgctt tttttatgtg aaattaaatt tattttaatc atttcaacaa
    26941 tgaaaaatat atttatctct attttgagac agaccgttct ttattttttt atctttggtt
    27001 aatttttaaa tatttaagta ggttctaata aaaaactcta tttagttaaa tatagttaac
    27061 ttgatata
//
LOCUS       DAEOSP010000033        26306 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.033, whole genome shotgun sequence.
ACCESSION   DAEOSP010000033 DAEOSP010000000
VERSION     DAEOSP010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.033"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(135..962)
                     /locus_tag="KRH54_001143"
     CDS             complement(135..962)
                     /locus_tag="KRH54_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG8578812.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(1010..2446)
                     /locus_tag="KRH54_001144"
     CDS             complement(1010..2446)
                     /locus_tag="KRH54_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBG8578813.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(2533..3402)
                     /locus_tag="KRH54_001145"
     CDS             complement(2533..3402)
                     /locus_tag="KRH54_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG8578814.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(3560..4213)
                     /gene="phoU"
                     /locus_tag="KRH54_001146"
     CDS             complement(3560..4213)
                     /gene="phoU"
                     /locus_tag="KRH54_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBG8578815.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(4241..5005)
                     /gene="pstB"
                     /locus_tag="KRH54_001147"
     CDS             complement(4241..5005)
                     /gene="pstB"
                     /locus_tag="KRH54_001147"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBG8578816.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(5024..5848)
                     /gene="pstA"
                     /locus_tag="KRH54_001148"
     CDS             complement(5024..5848)
                     /gene="pstA"
                     /locus_tag="KRH54_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBG8578817.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(5850..6767)
                     /gene="pstC"
                     /locus_tag="KRH54_001149"
     CDS             complement(5850..6767)
                     /gene="pstC"
                     /locus_tag="KRH54_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBG8578818.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(7148..7594)
                     /locus_tag="KRH54_001150"
     CDS             complement(7148..7594)
                     /locus_tag="KRH54_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578819.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMQIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIRTYCKHQVS"
     gene            complement(8111..8791)
                     /locus_tag="KRH54_001151"
     CDS             complement(8111..8791)
                     /locus_tag="KRH54_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8578820.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(8841..10856)
                     /locus_tag="KRH54_001152"
     CDS             complement(8841..10856)
                     /locus_tag="KRH54_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG8578821.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGQLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFEAYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            complement(10928..11605)
                     /locus_tag="KRH54_001153"
     CDS             complement(10928..11605)
                     /locus_tag="KRH54_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8578822.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     regulatory      complement(12185..12268)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(12501..13343)
                     /locus_tag="KRH54_001154"
     CDS             complement(12501..13343)
                     /locus_tag="KRH54_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG8578823.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(13595..15379)
                     /gene="pepF"
                     /locus_tag="KRH54_001155"
     CDS             complement(13595..15379)
                     /gene="pepF"
                     /locus_tag="KRH54_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBG8578824.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(15445..16110)
                     /locus_tag="KRH54_001156"
     CDS             complement(15445..16110)
                     /locus_tag="KRH54_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG8578825.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(16261..16422)
                     /locus_tag="KRH54_001157"
     CDS             complement(16261..16422)
                     /locus_tag="KRH54_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBG8578826.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(16500..17009)
                     /locus_tag="KRH54_001158"
     CDS             complement(16500..17009)
                     /locus_tag="KRH54_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBG8578827.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            17215..17433
                     /locus_tag="KRH54_001159"
     CDS             17215..17433
                     /locus_tag="KRH54_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG8578828.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            17451..19397
                     /gene="feoB"
                     /locus_tag="KRH54_001160"
     CDS             17451..19397
                     /gene="feoB"
                     /locus_tag="KRH54_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG8578829.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIIEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(19576..20631)
                     /locus_tag="KRH54_001161"
     CDS             complement(19576..20631)
                     /locus_tag="KRH54_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG8578830.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(20679..21479)
                     /locus_tag="KRH54_001162"
     CDS             complement(20679..21479)
                     /locus_tag="KRH54_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG8578831.1"
                     /translation="MNKLTKKELRSMFWRSFVLQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(21472..22263)
                     /locus_tag="KRH54_001163"
     CDS             complement(21472..22263)
                     /locus_tag="KRH54_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8578832.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(22303..22725)
                     /locus_tag="KRH54_001164"
     CDS             complement(22303..22725)
                     /locus_tag="KRH54_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBG8578833.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(22741..23220)
                     /locus_tag="KRH54_001165"
     CDS             complement(22741..23220)
                     /locus_tag="KRH54_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8578834.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(23352..26108)
                     /locus_tag="KRH54_001166"
     CDS             complement(23352..26108)
                     /locus_tag="KRH54_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8578835.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYENSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIFEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         8533 a         4611 c         2862 g        10300 t
ORIGIN      
        1 caagattatt ttaattttaa tgctttctca ttaattttat attatcctta cagtatacct
       61 cttattcgta ttttttatat tatatgtttt attttataaa taatttactt tatcttttac
      121 tatatttaaa tttattataa gttttcttgc cagaaatttt taggtgttat attttctgta
      181 attggcaata ttgtatatcc tctctctttt aagattctta taatattatt taaagcattt
      241 aaactttgct ctttctcatg aattaatact actaattcat ctctttttct tccataatat
      301 aatatattac ttactatctg acttgtagaa gacttccaat cctgtgtatc caaattccag
      361 tcccaaatta aataatcatt tgccataagt ttattatatg aaccttctgg catatatggt
      421 ttacttccgt atggtaatct tattaatttt gaactctgac cagttatttc ttgtagagta
      481 ttattgcatg tattaaattc cccaactgtt acttctgggc ttttatataa cttatgtata
      541 tcatgactta cactatgaaa acctattgag ttttcttctt ccaagactct ccttacttct
      601 tccttgtggt ttttcatatt ctgattaagc atgaaaaatg tcgctttgac atcattttct
      661 tttaaaatat caagtagtgc atttgtatat tttgatggac catcatctat tgttatgtat
      721 gctacttttt cagaagtagc ccctggacct tttgtttctt cttgagatgc tttgtatatg
      781 atttctccta aagtattact ttgagaaagc agttccagtg tattttcacc ttttgaactt
      841 atattttcaa ctggaacatt attaattttt tcctccaata atgttccagt taaaattaaa
      901 acagctataa gaaatatcct tataaataca tgaaaaacag ccttattatc tttaattttc
      961 atcccccata tctccctttt ataaaagcat aagtttaaga cttaaaaagt tattttttat
     1021 atttattctc tttcttatta ctttttatat ctctactaaa tattttttta accttttctt
     1081 cttctttttc tcctaaagat tctactcttt tgattcccaa agaagttaaa aattgtaata
     1141 catttaaatt atcaaatgga gtttccatat tcgtactttc actatattca tcaacattct
     1201 tagtaccttc ttccacactt aaaatacgct tcggacctcc tacacagcct cctacacacc
     1261 ccattccttc tataaatgta gcttctattt ctttatttag aactttcttt agaccgtcca
     1321 tgcactcttt tgttccttca aatgcctttt cttcaaattt tatatttttg tcaattcttc
     1381 taactgataa cttaactgct ttacttacac ctcctgttct agcataaact ctacctgaaa
     1441 gggacgcctc tactctatta ctctcatcca ttattgctgg atttaactct aatgcatcaa
     1501 atatttcttg caactcttta aatgtaagca caaaatcaac tgcatctttt atatcttcta
     1561 acattgcctc tttctttttt gccatacaag gacctatgaa tacaacttta gcatctggat
     1621 ttaaaatttt tacagctctc ccacaagcaa tcataggaga aacggatggt gatacatttt
     1681 ccaatatctg aggaaaacta ttttgtatca aactaaccca aataggacag caacaactag
     1741 ttataaagca tccttcttcg tcctttttca tatgctcata ataatcatat gcttcttttg
     1801 cagtaagcat atctgctgcc aaagcaactt ctatcatatc ttcaaatcct atctttttta
     1861 atgcagttct taatttacca gggcttacat ctttcccaaa ttgtcctaca aaagctggtg
     1921 caactattgc atatacaggg ctctttatat cttttaacaa ttttatcatt ggtataaatt
     1981 ctattttatc tgaaattgca cccaacttac atcttgatac acaatcacca caagcactac
     2041 atacatcttc ctcattacaa ttaatatgct tacttcttat cttacatgac tcatcttcct
     2101 tattattttc atcacaatat ttgcaagcat ctgatattac agatattatt ggtcttttga
     2161 cttcatctaa atttacagct tcatgtagtg actcttttaa atttttatct gtatcttctg
     2221 tcacatccaa tcccatagtt accctaatta aatttaccat agtatcttta tcaaattttc
     2281 cactaaaata attatttaaa ttatccttta aatcttcatt taaaatttta ttaggtaagt
     2341 tttcaatatc ctctatatca tcattccaca ctgcttttac aagttcgcca aaaagcttca
     2401 ttttatatat ttctagtatt ttagaattgt catttctagt attcataaaa aatagtacct
     2461 ccataaatta aacttatata tgtttaatat ttacaaagat actatttttt atttattatt
     2521 taactactat tattaatatg atttatttag ctttctcaac aactctattt caatcattat
     2581 tttcattaga tgatagttga atacagggct tttttcttca tctatattcc agtctagctt
     2641 atccacatta tatgttttct taaggtattt atatgtatgc tctttcaaat aaagtttatt
     2701 ttcaagtaac tctatagact gcatatgaaa atgtatttct ctacatatcc ttaaggcatt
     2761 ctcaattttt tctatattag tttcactcaa actataatcc aactcatcaa taagctttga
     2821 atatatttct tctaattttt ctacttcttc ttcaaatttc tctacattta aattttcttt
     2881 tctaagaatt ttatcttcaa taagcaacat tgaaagttct tctatctttt taaactcatc
     2941 tataacacta tttagataat ttggtctagc aacataataa ttaacaagta ctccaaacaa
     3001 aacacccaca gatgtatcta acaccctatg taaagaataa tatacagggt cactatttcc
     3061 tacacctaga tgtatagcca gaaaggttac acaggcaact actactgact ttattttaaa
     3121 cagattgcat aaatatattg agcacattac tcctagacca cataaaaatg ggtctccagc
     3181 tttaatgatg gcaaatatga atcctattat accacctaaa atagtacctt ccactctatt
     3241 gattcccgtc tttaaagaac cttttactgt atcttgaaca gaaattatac atgctaccag
     3301 agaataaaac atattctcta ctaaatagtc accaagaatc atacataaag tgacactaat
     3361 tcctgttttt atagtcctca tacccatctt ttgaagtttc aaagttccac ctcctaaaaa
     3421 gcaccttata taaaatacgc atcctcatat ttaatatgtt cctagtatat cacaattttt
     3481 taaatttacc cattaatcat atttttttat caattctttc ttaatttaga caataaaaaa
     3541 actgctttaa agcagttttt tatcctatct caaccatttt accctcaatt gcaaaaatta
     3601 tcttctcaca tacatttgtt atatggtctc caattctttc aaggtatctt cctataagta
     3661 gaagttttac cccttggtct atggtatttg agttttcatg cattattttt aaacaatcaa
     3721 tttgtacttg ttcatacaat ccatctacaa tatcatcttt agttccaaca tcataagcta
     3781 tacttgcatc aaataagatt aaagcttctc tagtctgaga catcatagac ttacattctt
     3841 ttgccatctt aggaatatct attagctctt ttatataagc ctctccacct atcttcaaca
     3901 tttctctagc tatatttgct gcataatctc ctattctttc cagctctata gatatattac
     3961 ctaatgaata aataaatctt aagtcccttg ccattggttg ctttaatact attagctcta
     4021 tactcttatc tcttatttct tctcttaatt tatttatttc ttcatcatct tctactactt
     4081 ttcttgcacc ctctaaatcc ttctctatca tacaagtcac agacttttct attgcttctt
     4141 cacatttttc catcataagc actgtttgtt cttttaaagt atcaatactt atatctaagc
     4201 tattattgac cattgtctaa ctcctttata gttatatatt ttaaccaaat ctacctgtta
     4261 tatagtcttc tgttctttta tctacaggag tggtaaacat agtttttgta tcgctaaatt
     4321 ctataacctc tccatttaag aaaaaagctg tttcatctga tatacgagca gcttgttgca
     4381 tattatgtgt aactatgact attgtgtagt ctttttttaa atcctctata agttcctcta
     4441 cttttaatgt tgaaattggg tctaaagcag atgtaggttc atccataaga ataacttctg
     4501 gtctcatagc aattgctctt gctatacata tacgctgttg ttgaccacca gaaagtccta
     4561 gtgcagatga tttaagcctg tctttaacct catcccaaat agctgcacct tttaagcttt
     4621 cttctacaat tttatctaat tgcttcttat ctcttagtcc atgtgtacga ggaccatatg
     4681 ccacattatc atatatactc ataggaaatg gatttggctt ttgaaatacc attcctactt
     4741 tagtacgaag atttataaca tcatctgagg tatatatgtc ctctccatca acagaaatgt
     4801 ttcctttaat tgttacatct tcgattaagt cattcattct atttaacgtt cttataaaag
     4861 ttgattttcc gcatccagat ggacctatta aagcagtaac cttattctct tttatatcca
     4921 tatttatttt ctttaatgct tgcttatcac cataaaataa atccaaatct tttacactca
     4981 tttttatctt atcgattaat tccattattc acacctctta aatttaataa ttagctttat
     5041 ttaatttctt agcaacaaat cttgctagca tgtttagtgc taacacaatt actattaaaa
     5101 caattcctat actggctgct gttgctatat ctccactctc tttagtcagt aagtatgagt
     5161 gtacagttaa tgttcttgca ctatcaaata ttccacctgg catcttggct acagtacctg
     5221 ctgtaagtaa tattgcagct gattctccaa ttatacgacc aactgataag attactccag
     5281 ataaaattcc tggtattgca cttggaagta ctaccttata taaagtttga aattttgtag
     5341 ctcctaatgc caatgatgat tctctataag cttgaggaac tgtttttaaa gcttcttctg
     5401 ttgtacgaat tattactggt aatattatta ttgcaactgt aagtgaacct gcaactatgg
     5461 aatatcccat ttttaatgta actacaaaga aaattcctcc aaataatcca tatactatag
     5521 atggtatccc tgataaactt tcagttgcaa atcttatagc tgtaactgct tttccttttt
     5581 tagcatattc ttgtaaatat atagcagcca atattcctat aggagttgct acaactatag
     5641 aaactactac tgtatataat gttgtaatta tcattggaag tataccacca tcacctgatg
     5701 ctgaataatt gctaaataag aaattaaaat tcatgttgcc aattcccttt ataaatataa
     5761 atcccactat aactactaat gatactattg taaatagtgc tgatagatat actaatgact
     5821 tcaatagatt ctctttgaac tttctcatct taatttaccg ccttatttga aagtttattt
     5881 aaaactaagt ttaaaattaa tataaatgta aacaatacta ctccagttgc aaatagcatt
     5941 tcttgatgtg taccaaatgc gtatcccatt tcaagtgcta tattagttgt aagtggtctt
     6001 acactatctg ttattgcagt tggcataact ggtgagttgc ctgcaacaag tattactgcc
     6061 attgtttcac ctaaagctct tcctactcct aacactactg cggctaatac tccagatttt
     6121 gcagcaggta atacaacttt gaatattgtt tctatttttg atgcaccaag tgctaatgaa
     6181 ccttctttgt atgcttttgg tactgctctt attgctgttt ctgatacaga tattattgtt
     6241 ggtaacatca ttactgctaa tactattact attgctaaca aactttgacc ttttggaaga
     6301 ttaaatatac tttgtatagt tggaactatc actgcaagac caaatacacc atataatact
     6361 gaaggtatac ctgctaaaag ttcaactgct ggtgacatta tttttgctaa tcttttaggt
     6421 gctacttctg ctataaatac agaggttaat ataccaacag gtactcctat aacaagtgct
     6481 ccaagtgttg caaatattga tgctactatc atagatgata ttccaaattt attggcagaa
     6541 ggaacccaat ctgcaccagt taaaaagtct ataaatgaat atccttcaaa tataaatggt
     6601 cttaatcctt tataaaatac gaatcctatt atcaaaagta aacttgcaac tgctactaaa
     6661 gcactaagta gaaaaatatt ttttgctact ttttcaatta cgtattttgt cttatttcca
     6721 ttattgtcat ttttttctaa acctactaat ctagatttta ctatcataat accctcccat
     6781 taaaatatac ttatcgattt tcgttctgta ttcaaaattt aattttttat aattcgtctt
     6841 catcaaattt cctatacttt tattctactt tttttaagtt aaatctatat taggattaag
     6901 ttaagcaagt gtaaaattat atttactttt taacattaat tttactttta aaactatatt
     6961 tgacatttac tttacaatat actgttgttt ctctatatta aaatagcaga tatagataat
     7021 aatacagttt atgtataatt tattacctat atctgctatt aagatttgtt aactaaattt
     7081 aacttttata ctacatatat aattattgta tcctctagtt tatatcacta ttatttccat
     7141 caaattgtta cgatacttga tgtttgcaat aggtacgaat agccaaaaat ataaaaatag
     7201 gtatttctat cactgagaaa aacagtataa tgtgtggtgt ccaaaaactc atacctccta
     7261 agtgtaagaa attaaatgat gtcatttgat ataataaact aatcttaaaa ccaattcccc
     7321 ctggtggtaa taggtatcca atccaactaa cacctattcc cataggaatt aagcaaatag
     7381 aaaaagcaat ctgcatagaa gtaagagaat ccttacattt tgcagataaa aataatgtta
     7441 aactaaccat tgcaagtatt gaaattagac caccaatagc tataagaatc tgtgcttgta
     7501 gtaaatttat atttactaga ctaatgacag aaaacaacat ttgaaatgag gttttcaaac
     7561 attcagtacc aaaagctaaa tcagaaattg ccaaatggat agaaatacat ataacaaaca
     7621 ttattataaa tatagaaaat agagctaata ttttagtaaa cgctagacga atatgtccat
     7681 gtttcgtaca acgaaggatg ctatctgatt gtgtctgata ctcaccagaa aaagtaggtg
     7741 cagcaattgc aacatatata attaataata taaaaatata aaatattata taatcgaaag
     7801 catcttctgg atttattaag aaattcttaa tcttattatt taactatttt attatttaat
     7861 atatattaaa ggtaaaaaat aatatataaa tttagatata caatttattt atcttttcaa
     7921 atataatcta taaaataata gttccgttac gcattttttt atataataaa actgactgta
     7981 atttaactgg caaagtctag aagaataggc tttatcaatt aaattacagt cagctatttt
     8041 ataataattt agtttagata tcgttagcat ttcacattta taacaattat taatgatatt
     8101 aatatattta ttattcctta ttaaaataat atcctgctcc tcttattgtt ataattttct
     8161 gaggattttt ataatctttc tctattttct ttcttaatcc attcactgca actaccacac
     8221 tatagttatc ctctaagtat tcattcatcc atacatgttc atatatctct ttagtagtca
     8281 aagtcttttc aggattctct ataaaaaagt ttaacaactt attttctgtt ggagtcaatt
     8341 ttattatttt atcttctaac tttattactc cactagttct attatattcc tctaattttg
     8401 tattattttc attattattt acatatgagt tcattcttct taaaatagct tttacttttg
     8461 catataatat ttctaaattt agaggcttta ttaagtaatc gtctccacca gaatttaaag
     8521 catttaaaat gctttggtct tcatttatat aagtcatata aacaattgga caagtagtta
     8581 catttctaat ttttttacaa agagtccatc catcaccatc acttagaatt atttctaaaa
     8641 atattaaatc atatttattg ctaaatattt ttccaatagc ttcttcacaa ttatatgcta
     8701 aatctatact tattcctttt tcctccagaa atccttttag cttctggcaa ttatccttgt
     8761 ctccatctat tatcaatatc ttagtttgca ttataatcac tcccaaatat cattattctt
     8821 ttatattaaa taagctatta ttataaaaaa catagactta attttattgt aaaaacagtt
     8881 ccttttccta gttctgaatc tacaaatatt tctccaccat gctctcttat aatggatttg
     8941 acaatatata gccctaaccc ttgccctttt ggctcattat tgtctctttc tgtaaagaat
     9001 ctgtcaaata cctttggtat attttctgga ggtatacctt ttccattatc agaaatttta
     9061 ataattgcaa atccatcttc tttatccatt gataaaatta tctttccatc aaatggagta
     9121 aaggtcaaag cattataaat taaattctcg aatgctcttg taagcatctc tttatttatc
     9181 tttgcatata tttttttctc caatacgtaa gcttcaaagt ttacaccaca tgcttgtgta
     9241 tctggcaaat tattttcata tatctcttta ataaaattat tcagacatat tttttcacta
     9301 ataccaatgc ttttatcttg tgatgtaaat tcttgcaaac tacgtactct attttgtact
     9361 tcatttgact tttgatttat agcataaaga taggttcttg tctcttcatc tacatgaata
     9421 ttaccaacct ttatcaaatc tataaatgct tgtatagccg aaacaggtgc ctttaaatca
     9481 tgtgctacat ctgccaagaa tcttttacgt tcatttaaca atgttgtaag ttcattagtt
     9541 ctcacttcaa cttcatgttc aagattcctt gtaagttttt cattctcttt tattattatt
     9601 ctattcctgt ttatcatcat aacagaaaat aaaataacaa ttacaaaccc acaatattct
     9661 gtttgccacc ctgtaaatat aggctcaaat ctattactcg taactgcatc aaataatatg
     9721 ctaattccaa aaacagtgtt tactgaaact aaaatataag aacttatgtg ctcagagcat
     9781 gtctttttat atattgaaac cagcgaagac attaatatat atataaatac aatatattta
     9841 tatatgtcta ttaacttccc atatatgttg acaaagtaca tatcatatgg aagaagaata
     9901 attggtatca ctatagatat gatactcatt gtaattccta atggtaaaat taaaaatcta
     9961 tacactttat tttcttctat tttggatata cttccattta tacaaataat acataaaact
    10021 accataaaat atgacaaatc ttcaaccgca tatgtaaagc ttactaaagg cagtcctaac
    10081 aaatgtacaa agggatagga tacatggatg gcaaagaaaa cacacataca tccaaaatat
    10141 aagtaaattt tacttttgtt tgaaagaaac catacagaca atgaaaatat agctattgca
    10201 aaagttgtga agcaaattat actataaaaa agtaatcgat aaaacatcat attacttatg
    10261 tcctttgttt ctcctaatgc aggcggataa tacaaaccac tataataatg tgaaaaattc
    10321 gatacattga ttataatttc tgtatttttc tttgcaataa aacttactac tgaattttta
    10381 attttgggtc tatatttttc tgttcctaca tctcctaatt tagaaaccat ttctccattt
    10441 atccatactt cacttgcgga aaaaatctca ggaagctcta aacttagtag cttatccttg
    10501 ccttcatttt ttattctcat acggtaagta gcattcccat atggtgactg attccctttt
    10561 ctcatagaaa aatctggata ttgacctata aaaacatatt ctggttttat atcttgctct
    10621 gtaaaatcat tcggtttaaa tattttatga ttataaaact cccagtcatc tatcaaaaag
    10681 acaagttgcc ctttagtgaa agtatcatta tctatagtta aaattccatt acttgctcta
    10741 atagacttat atgtatattt attatcatat ttatacagaa gatttaatcc tgttattgcc
    10801 aacagagaga ctatcaatac aatgagagtt ccttttagta tattatactt tttcattgca
    10861 tcaaccacat ctctttcata gtcgtattaa ataaaacaag aactatttat taattacagt
    10921 atctcactta tggtacaaac ttatatcctt ttccccatac ggtttgaatg tattgatgct
    10981 ccctgcttaa agaattaatt ttttttctca tctgtgcaat atgaacctgc actgtatgtt
    11041 gatctccaat atcaggcatt ttccaaactt ggttatatat ctctatcttt gaaaatactt
    11101 gttctttgtt ctcagctaga aacaataata tttcaaattc catagtagtt aaatctatac
    11161 tttctccatc aaaagttact tttcttgaat ttacatttat tactaacgct gaaaacttta
    11221 acacattagg aactgatgta atatttttgt actgcctcat acgagcatat attctaagtt
    11281 caagttcttt taaactataa ggttttgtta tataatcatc ccccccagct aaaaatcccc
    11341 gaactttttc ttcttcatgc tcataattag ataaaaatat tataggtatg ttaacctttt
    11401 cttttatatc attgcacatt ttatatccat ctatatctgg caatgaaata tccaaaatta
    11461 tacagtctaa aactatactc tctactatat ccatagcatc tttagcagtc tctgatgtat
    11521 atacattaaa acctatatta gaaaaataaa tttgatttat gtttaaaata tcaacatcat
    11581 catctacaac cagtaaatta tacattacac cactccattc aaaggtattt taattataat
    11641 tccaagtttt atagttatta caaattataa tgttttattt tcatatataa ctggtattat
    11701 tattttataa tatctttaca aactagacta taatacttaa gtatttttga ataattcttg
    11761 aatattgtat taaacattaa gattcattat taaatactaa ttaaagtctc cgattgttgg
    11821 agaatggagc ttaaagtcat tttcacgtat aaatttatta tttttctaga aacttgtcta
    11881 ttggctagtt tgagtattga tttatatgat ttatatggtt ataatataaa ataaattgta
    11941 ttattttggt aaatatattg ttacaaaagg tttatattct gcatctcaat gtttataaaa
    12001 actttatttt cttattataa tactattatt cttttaaaaa aatttccctt ttccaacaaa
    12061 tggctacttt aaaagccaaa attttaccta tcaataaaaa tcttaacgac taatactctt
    12121 attttcaaat tttgcaacat ttttgttaat tttaaaacaa aaaaagacta tgcaatataa
    12181 aaattatatt catagccggt tgcaccacta actccatata tctccagatg cccaataata
    12241 agatatactt ctcagtttct aaaatatttt tattaaaaat cttttatata ttttcataaa
    12301 tttctttttt attctaacat tgtaatatta ttttgtcaat atttccatat tcttagatat
    12361 aagatactaa aatacctatt ttatctaaag atatttatat ttctacattc aataatagac
    12421 aataccctgc aagttaaagc atcattttta tgagcttatc tcacagggta ttatatgttt
    12481 actttatatt ataactcttg ttattgatta actttaataa atcctttttc tgaaacaagt
    12541 gcttgtcctt catcacttaa tatataatct attaaagctt tacctttttc tgacaaacca
    12601 tcttctttat taactaatat aaatggtctt gatattggat atttattagc tattacattt
    12661 gcatcagttg cttcaacatc atttattttt aaactattta ctttctcatc aacatatcca
    12721 aatgatatat atcctatagc attttcattt ccttcaactg tagtttttat atttcctgaa
    12781 ccatcactta tttgtgcatc tttagttagt tcttctgaat caaaccctac aatttcttgg
    12841 aatccatctc tagttcctga accttcttct cttgatacta ttactattgg tgcatcattt
    12901 cctcctactt ctttccagtt agtaatcttt cctgtatata tacctttaac ttggtccatt
    12961 gtcagatttt ttactgagtt tgcagtatta gttatcaatg ctataccatc tattgctatt
    13021 ttagtttcct ttaatccagc acttttttct tcatctttta aatctcttga actcattcct
    13081 atttcagatg ttccttctat agcatttttt atacctccag aagaaccaat ctgttgtact
    13141 tctacactaa caccttgatt tttttcttga aacttttctc cgatagtttc cataagagga
    13201 cctattgaag ttgaacctga aacagaaact ttgcttgaat ctcctgaatc acctgaacct
    13261 cctgagttag aactacatcc tactgctaaa gctccaacta acattgtact agctaaaaga
    13321 acgcctattt tctttttaaa cattttactt acccccgctt atattttaat tttttgttgt
    13381 catttttcta tattttaata atacagattc tttgttaaat gaatattaga gcagggttaa
    13441 gtaaatgtaa atattgttaa atacatgtaa aaacttaatt ttatatgatt tattcttaat
    13501 tagattaact ttttaaagtt actttaggca gatataaaca taatacaact catattatta
    13561 gaagtcttca ttgaagctat caacctatca catcctataa ttctttttct agtttattaa
    13621 ctaattcagc aaatacattt aaggcttcct ctatagattc ttttttcgtc atatctacac
    13681 cagcagattt taattgttct agtgggtatt ctgaccctcc actttttaag aagtttatat
    13741 atctatctac tgcagtactt ccttctgaaa gtatctgttg acttaaagca cttgcagcag
    13801 aaaaccctgt ggcatactta tatacataga agtttgaata aaaatgaggt attctcgccc
    13861 attctattcc tatttgttta tctactattg cagattttcc gtaatacttt tcatttaatt
    13921 tgtagtatat attagtaaat tcatcagcag taagtggttc tccactctct accctttgat
    13981 gtacaatttt ttcaaattca gcaaacatag tttgtctatg aactgtagtt ctaaattgtt
    14041 ctaaataata atttaataag tatatagttt catccttaga cttagaattt tctaataaat
    14101 aatttataag aagtaattca tttaatgtag atgcaacttc tgcaacgaat atcttatagt
    14161 ccgaatataa aaatggttga cttgttcttg aataatggct gtgaacagag tgccctaatt
    14221 catgaattaa agtgaataga gaattaaggt catttttata gctcattaat atataaggat
    14281 gtgaatcata acttccccaa gaataagctc ctcctttttt accttcatta tcatatacat
    14341 caatccaccc ttcatcaaaa gcacttttta tgaccttcaa gtattcttct cctaaaggtt
    14401 taagtgcctc taatatgata tcctgtgctt tatcatatgg tatatccatg tcaaatttat
    14461 ctgtcaaagg aacatacaaa tcatacatgt gtatttcatt caatcctaaa aatttctttt
    14521 taagctcaac atatttattt agattaggaa tacttttatc tatagctgat ataagattat
    14581 tgtaaacatc taaacttaca tcatctgaaa ataaagacgc ttctattgct gaattatact
    14641 ttctagtttt tgcgtaaaat atctctgatt ttattccacc ataaagcgtt gaggcaaatg
    14701 tatttttata tttctcatat gttgaaaatt caccttcaaa agcgtctttt ctaactctac
    14761 tattcttact ttttataaat aaagagtagt ttgaatgaga aagcttaacc ttattcccct
    14821 cctcatcttc tatctcagga aattccatat ctgcatatgc taacatatcg tatacatttt
    14881 ctggaattgt cgataattct gatgtagctg caagtatttc ttcctctcgt tcactcaaag
    14941 tatgtggttt atctctcaat atatccttta tatattttgt ataaaaggat agcttctcat
    15001 cttttaagta ttcacttaac ttattctctt ctatcgccag tatttctgga actatataag
    15061 aagtagccac acctaaatca gtagataaca tttctgtctt agtggctctt ccttgattat
    15121 cattaattct agtatcttca tgttgtttca tatgagtata tacatataaa ttttgaacaa
    15181 tcctagatgc attttcagag atatttaata ctttatataa gttctcctta ctctcagaaa
    15241 gcttcccttt atattctttt acttcttgga taagattttt tactttactt atgtcttttt
    15301 caatttcctc tttactggag tacatcttat ctatcttcca tttgtacttt tcttctattg
    15361 tacttctatc agttcccata tcatacacct ctattttaat ttgttttata ttttattcac
    15421 tttacaaaat atattgtata tattttagtc caaataatct acatttatta ctccttctat
    15481 catatttatt tctttcataa cgtcgtgtaa gtgtattctc ttagcataag atacaaaaaa
    15541 attaaccttc cagtattctt cattacgaca ttctatttcc atatttctta cgataagttg
    15601 atttttttca aatttatcat ataaaaaagg tatagaatcc aaattgctta attttaatat
    15661 caatacattc ttttcttggc taaataaaaa cgtattgatt atcctaagca caaataatgc
    15721 tatcattaca aataatccac ctagaattgc caattcataa taaccatatc caactgctat
    15781 accaatacat gcagttgccc acatcccagc agctgtagta agccctttta ctccaccttt
    15841 attttttaaa attgctcctg cacctaaaaa accaattcca gataatactt gagcaggcat
    15901 tcttgctggg tcagcatttg tatacaaatt ataatcaaag aataattgaa tcgaagttat
    15961 tgaaacaata caagaaccta ttgcaattag tatatgtgtc ctaaatcctg caaactgtct
    16021 tattttttct ctctctaagc ctattattcc tcctattatt agtgctaata gtaaccttat
    16081 tgctatctcc ttacttccta tatcaatcat aatataaaac cctcctgact taattacaac
    16141 ttatgtcctc cagataattt acacatcaga aatactattt tatatcttaa tattattatt
    16201 aatatatgta aaatatagcc atctgttaat ttttgataca aaaaaaccca caactgtggg
    16261 ctatttgctt aggtacatgt atttatttat taatatatct attttcttac ttgattctac
    16321 taactcttct tgaaatattt ctggatattc tatgtatgta tttatctctt ccctaactgc
    16381 ttcaatctca ttttttaacg cttctaattc attgttcgcc ataataatca ccactattac
    16441 aaatttgtat atttttatat acattttatc aaacaatatt atattttgtc aatattatgt
    16501 caataaaact tactataaaa atgctttaat ttaggtattg tatacctgga tactaagcct
    16561 acaaacatac cagttactaa tgatgccatt aacataaatg gtaagtaccc tatagcaagt
    16621 atattttcaa tgattaaact tgcaactatt aattgaccta tattatgccc tattgcacct
    16681 ataatgctta tccctattag tgataaatct tttattttac tcgctaaata cataaataaa
    16741 agacttagat aagctcctcc tatactaaat agtagatatg ataaagggcc tgcaaatatt
    16801 gatgacaaaa ttaccctgat agtaacaatt ataacagtat ctttgacacc taacaacatt
    16861 aatgaaatta aagttataat attgctcaaa cccaattttg cccctggaaa tataactata
    16921 agaggatttg gtatatatgt ctctaataca tataaagcta aactataccc tatcataaga
    16981 cctaaaaatg tcatttttct agttttcatt aagttaactc ctccaaattt tatctatcat
    17041 cttacattat aatacaaaat atataaaaac acctaattta gactaaaata tttttaatct
    17101 atttagaata aatattagtt atttcaatgt gcaattacct ctataaatct tgactttttg
    17161 tatcatactt gttacaattt taatgataat gaatttcatt aggaggtgtt aataatgact
    17221 gtttacgatt taaaaatagg agaaaaagga tttattaata gtatagatgg agatattaaa
    17281 ttatctaaaa gattgcttgc acttggatgt ataaatagta ctgaaataga agttaaaaag
    17341 gttgccccac ttggagaccc aattataatt agatttagag gctttgacct tgcgattaga
    17401 aaatctgatg ctaaaaatat ttctttacaa taatagagga ggagctaccc gtgattaatg
    17461 tagcattatt aggaaaccca aatgtcggta aaactactgt ttttaatcta cttacaggtt
    17521 caaatcaata tgtaggtaac tggcctggtg ttactataga aaaaaaagaa ggatttcttg
    17581 gaaaagagat aaaggttgtc gatttacctg gtatatatgc aatggatact ttctcaaatg
    17641 aagaaaaggt ttctaaatca tatttagaaa atgaagatgt tgatgtaata gtaaatgtag
    17701 ttgatgcttc taatttatca agaaaccttt acttaacaac tcaacttatg caatttaaca
    17761 agcctatagt tatattgctg aatatgttag acattgcaga atctaaagga gttaatatag
    17821 atgccaaaaa attaagtgaa gaacttggag taattgttgt tccaattata gcaaaaaaga
    17881 aaaatggtgt tgataggata gaagaagaga ttaagggagc tacgcaaaaa gcttttatat
    17941 atcagaaaaa ctttggaagt gaaacagaaa catataaaaa attagaatcc attttatcaa
    18001 gatgtacaaa aactacatat acacaaaaga aatctatcag tgataagata gacaatatag
    18061 tcttgaatcc tatattagca tatccaatat ttattggtgt attgttcctc ttatttaaat
    18121 ttacctttga ctgggtcgga ggtccactac aagagggatt tgctagtctt atagaggctt
    18181 atatttctgc acctgttagt gatatgttat ctaactctag cccatggttt aaatcactta
    18241 tagttgatgg aataattgga ggtgttggtg gtactttacc attcttccct ttaatattta
    18301 cattattctt tggtatatcg ttgtttgaag atagtggata tatgtcaaga acagcatttt
    18361 taatggataa agttatgaga aaggttggtc tatctggtaa agcttttata cctatagtta
    18421 tgggtatggg atgttcttct ccagctataa tggcaactag aacattggaa agcgaaaaag
    18481 atagaagagt tacagctctt attgcaccac ttatgacttg tggagcaaag ctacctatat
    18541 atgcactatt tgtagctata ttctttccac ataatgctgc tttagtaact acatcactgt
    18601 atttggtggg tatagttatg gcaattgtag ttgcattatt cttaaataaa acagccttta
    18661 aaacagaggc tgaaccattt atattagaat taccagaata tagaatccct actatagatg
    18721 cacttattaa aaatacttgg aataaatcaa aaggtttctt gataagagtt gttactgtca
    18781 tgtttgcaat gtcagtcgta atttggggac tttcttcatt caatgttttt ggatatacag
    18841 aagatataaa tgtaagcttc ttggctactc taggtcatat tatatctcct atattcaaac
    18901 ctcttggttt tgatgattgg agaacttcag ttgctatttt aagtggtctt ggagcaaaag
    18961 aaatcgtagt aaactcttta aatatactat atggtaatct aactgttgtc ttaccaacaa
    19021 tatttaatgg agttactgca tatacattct tgatatttac tgctttatat actccatgta
    19081 tagctgcact tgcaactatg aaaaaagaat atggcaataa aatgatgttt acatcatttg
    19141 catatcaatt tatactagca tggataatgg cttttatagt taaaagtata ggtggagtac
    19201 tatttatggg taattcaata atagaagctt tgataggtgg aataattata gtccttgcac
    19261 tctttattct gtttaataaa ttcaagtcta caaaaaacgg aaatgtctgt agtggatgtt
    19321 ctatttgttc tggatgtcca agtgctaatt cttgctcaag taaggaagac agcaataaaa
    19381 aaaataatgc acactaatat ataaatattt tagtttaaaa taagataaaa gtgggtgtct
    19441 caaaattaac tttttagttc atgaggcact cttttttata gaaaatcaaa tatattttca
    19501 tcatttcaac agtaaaaaag agcttatcta gtaaaaaaga ggcttatctc tattttgaga
    19561 caacctcttt ttttgttact tattcagttt cttgctacct aacttcatgt ggaacctagt
    19621 atcttttgga acacttgggt ctacacctct taatgtaggt atttccccac atattagttg
    19681 tataggtact ataaaattaa atgtttgata gtaaggatta tctgtgatat caatctttaa
    19741 atttttatca ttttctgtaa catctctacc tatagcaaat acattttccg tccaaccaga
    19801 aagtacatca atcatcttag ttactctagg ttcttttcct gtatctagta taaatatagt
    19861 agaatcacta tttatagcat tatatatacc atgtataaac tcttcaaact catacccagt
    19921 tacaggtatt ctcattgttt ccaataattt taatgctgct tctaatgtat ctccatatat
    19981 atcagagtga cctattattc ttatttcttt agaattaact aacttttctt tgtttctttc
    20041 aatccattgt ttagagagct tatacactgc ttcaaatcta ttaattgcgt ctaatatctt
    20101 atttatttcc tcattgtact tttcactaga tattatacct ttttctctag ctatttgaag
    20161 acctaataac attaagttta actttgtaca ataatatcct tttgttttag ctccagattt
    20221 ttcttcacca caatttactg ttaatatata atcagaaatc tcatctatta aagcattctt
    20281 acaacctgcc atagatgcta ttttacagcc cttatcttca gctaacttca tagcgttgta
    20341 tgtagaataa ctactcccac cttgagatac acctactact agggtatttt cattatcaaa
    20401 cttgaatgta tcttcagtta tcataaatgg atacattttc acaacatcta tatctaataa
    20461 attttgtaaa tatggttgca cttgcactcc tgagtgatag ctagtacctg aacctgttat
    20521 tataatcttt tttagatttg ttctcttaac ttcattaaat aagctatctg cattagatat
    20581 aatttctctc attctaactg gtgtttctaa catataatct tgtattgtca ttttatacac
    20641 cttccttatc tatatattca ataaattata attaatttct ataacaaacc tatagcatgt
    20701 aaagcgattc ctgttactat agtgcctata gttaattttg ttacacttac ctttctcttt
    20761 aacatgtagt atattacaaa tgcaaaggtt aaaggtaaca tattaggtat tattttatct
    20821 agtatatctt gtaaaattac ttctgcacca tttaagttga atttcagagg agtagttata
    20881 ctcaacatag ttgcaaccat tcctcctaca acgaataaac caacagttgt agccatagac
    20941 attattttct ccataagtcc ctctctttgt attctttcaa gagaatttac ccctacttga
    21001 taaccatact taagtccaaa tattctaagg gcaaatgctg gtatattgta taatagtaag
    21061 aataatagtg gtccaaagat attaccttct tttgcaagtg atactcctac tcctgctgct
    21121 ataatcctaa atgttcccca gaataatgaa tcaccaattc ctgcaagagg tcccataaga
    21181 gctgttttaa cagcactaat tgaactttca tcaaattctg gattattagc attctcttct
    21241 tccatagctg ctgttatacc taatatagca ggaactacta ctgtcgttgt attaaaaatc
    21301 tccaaatgcc tttctattgc ttttgcttga ttttctttct tattgtatag cttctttatt
    21361 gcaggtaaca ttgaataaca gtaacctaag ttttgcattc tttcgtaatt aaatgcacct
    21421 tgaagaacaa atgaacgcca aaacatactt cttaattctt tttttgttaa tttattcaaa
    21481 gtcaatctct ccttccgtta aagtattgat tgctaattga ggattttgat tattttcttt
    21541 gttattgata taaaagttta gtacaaatgc tacacatact ccaaataaag ctatagcagt
    21601 tatatctaat ccaccataag atgctaacaa aaaccctata aagaaaaata cagacatctt
    21661 ttttgaaagt aacatatcca taagcaatgc aaaacctact gctggcaaca atgttcctgc
    21721 aacacccagt ccgtctaata taactttagg tattgcattt atcaatgctg taactttatc
    21781 agcaccaatc ataattgcta aaaaggttgg aactgatgta cttaaaaaga ataatattac
    21841 tggcaaccac atcattatgg taactttctt aaaatcagct tgtttagcat agaaaactgc
    21901 cttttgagaa aagtacgaat ttattattct aactaatact ccaagagatt gtgctaagac
    21961 tgctactggt actgctactg ctaaagcaac ttctgcacca ttacctgata atatagcaaa
    22021 agctgtacct aatacgcctc ctgttactac atctggagga gtagctgcac ctattcctgc
    22081 aattcccatc caaattaatt caagctgtgc accaatcata attcctgatt ttatgtctcc
    22141 taaaactaat cctactaaca tacctgtaac tattggccta tcaagcatag attgaccaaa
    22201 tatacgccca tctagtatac caactcctgc tataatagct aacatagtag cttgtactaa
    22261 catattgaac ctccatttta acttagttta atttacatta aattacatta catttttgat
    22321 tttgttttga ttttctaata actcatttac ataaacaatt ccattttttg cttcctctaa
    22381 tcctgattta actattagtt ttgtatcctc ctcaactaat aaaagtatag ataaaagcat
    22441 tggtaaattt actcctgtca ataattcaaa tttgaaccct tgtaaaagta agtttgtaca
    22501 tatgtttgcc acacttccac caaaaacatc tgttaagaca acaaattctt cactatcttt
    22561 attatttgat aatatacttc taatttcttt ctcagcatca tctgctgatt tatccgttgt
    22621 catacaaaaa tactcaatat tagttaatga accaattatt aatttacttg tgtcaacaag
    22681 ctctctggac aattctccat gtgttgcaat tagaaatttt ttcattctct ttctcctaaa
    22741 ttattttatt aaatctgata gttgtttctt tttgtctcta ggtaacattc taaattctat
    22801 atcaatccct aaatcaccta tttgtttaaa gttatttata tcatcatcat caacagcaac
    22861 taaatctgta agtttcttct tcccttcctc atatcttaat cctcctacat ttatttcttt
    22921 taatactcct cctgaatttt tagctacaac taaagcatct ttggtatttt ttactaacac
    22981 taatacattg cttttctttg aagaagcaaa tttttctact atttctccag attcctctac
    23041 cccccttata tataaagtta cattgctagg tttcgctaag ttcagtgaca tagctttcat
    23101 ttggtcattt tttgcttcat catttgcaac tagtattaca tttataccta gtgatgttgc
    23161 ccatccatat gcaacctgac catgtatcaa tctgtcatca attctcatca aagataccat
    23221 tttccccaaa tcctccatca tacattaaaa tttttattgt ttatactctc atttatctat
    23281 aaaagcaaaa agtatgccaa atactggcat acttttttaa atttcattca cttattttgt
    23341 aatcttgtat tttactgtgg tagaccatag ataatattat aaatatatcc tatctctgaa
    23401 attggtattt gcacactata actcttttct agcacactaa atgcattttt tattagtttt
    23461 acttcactat gatggttttt gattaacaaa tccatatcag tatactcttc aatttcagtt
    23521 tgtctaacta atctttcaat catacaactt aagtgaacat ataaagaaat ctttttatca
    23581 tttgaaagtt ttttcttttg aatattttct atagcattaa ttccttcttc tattctctct
    23641 attatctttt ttgaatctaa aatagttaga ctatctatta ctctaattaa tgaaaaattc
    23701 tttacaatag aatcatttat gttctttatt ttttcatttt ctactactct gctaaatatt
    23761 ctaaaaacat catcttctcc ttttcctgat attatatcct ctaataatat aaaatctacc
    23821 ccattcacat ttggattggc agttcctact atagccatca catcatacat ttcaaaaact
    23881 ggttctttat ctcccataga aacaagacta tcataatcat aaggtattat tttgacatca
    23941 actattccaa ttaaactttc ggaaagtaga tgttggattt ttatagcagt acctatccct
    24001 gtaaaacaag atgatattat agcatattct ttattttttt gaggataata caattgtata
    24061 ttaggtttag tttcttcatc cataatttct gcaatctctt caatgttttt accttgcatt
    24121 aataaatttc ctacttctag tgcagaaata gtggatattt ggtctataaa taacattggg
    24181 ctatttatct tattctttaa atcttcaaat ataagattaa gagagcccat atcaaataac
    24241 acaattaaac cattctttat cctatctccc tctaggtatc tagacatttg ttttgataca
    24301 tcagcaatag tagcatctat aggcatatca atagctgtaa atatattttt acccaacatc
    24361 cgattacata cattcgccat actactggca gtagcatatc catgtgctat aatagtcgca
    24421 ttaacttgat taaatttcat tcttataatt gttgatttaa taaatataat cattatgatt
    24481 gtatctacat tattaagata catatccaat ctattcgtta ttaatgttga tattttactt
    24541 attatagagt attctttgta actctctttt tttaaatata ttagtaattt attttctaaa
    24601 gctttatact catcagacaa ttgataagat tcgtccttca aataaagata agacgaaaat
    24661 gccaatacat tattcccatc atattttata ttgttattaa cttctaaatt tctaaatata
    24721 tcctgcatta tcgatattat aagctccata agtacgttat tattttcatc attatttgaa
    24781 aagactaaat tgtccattag tctaatcatt agattagaac tctttttact aaatttttcc
    24841 tttgttattt catttttttc tagtctatca aagtaatcta aacattttat gaaactttga
    24901 tagattatat ttcttttatt tcttccattt gacatttcaa tactaaattg tttaggagaa
    24961 aagataatgc ttttgctttt accactttgg aattgaaata tatctacatt ttctatataa
    25021 agatttctag gaaggtcttg aagagtaaca aaaatatttc catttcttct ttgatttttt
    25081 gcataagctc gtccacatgc atattttatt acatttttac attcacctat attccccttg
    25141 aattttatct ttaaaatcgc atttaatgta atttgagtta tctctatagg caaaccaagt
    25201 accttacttt cttcataaat aaaatgttct acaatctttc ttttttcaat agtccctctt
    25261 tcttctatat tgggaacata aatatttatg ggtattcttc ttaaaaaggt ttgtaaaaat
    25321 tcagatagac tttctgttgt agcaaatatt aatcttaccc tagcttttct aattactcca
    25381 ttctctccaa tcctagaaaa cgtacctgta tccataaatg taaataactt ttcctgacct
    25441 tcactattta atctatgtac ttcatctaaa aataaaacac caccatctga ttgttctatt
    25501 agacctgctc tagattcatt tgctccagta tatgctcctt ttacatgccc aaataataga
    25561 ctagacaata actccaaatt attataatac tgcgcacagt ttaacacatt gtatggagaa
    25621 tttttttcta atacattact gcttacacta tatttgtata ttatcttggc taaaaaagtt
    25681 tttcctactc cactttcacc tgtcagcatt attgtaagtc cgccatttgg ataaagtaca
    25741 gcggttttta tttgttcgat tacttctttt aagctattct catatcctat gatactatta
    25801 aacacagtct cttgctttaa taatttacac tcatttttaa gttcatctaa attttcataa
    25861 atactttttt ttgtaggtcc tactacatca tttattttat ttttaggtaa aaaaataaca
    25921 ggtctactgt ttatctttat aagattatca gattttacta aatcatttaa taatctactt
    25981 gattgagttc tacttaaatt aaactttttt actatatctt ctgttattat gccactttct
    26041 tttttaagtt catcaaagtt gttaaataat acacattcat ttacaaaact taaaatttca
    26101 tccctcatgt tcaacctcca atacaaatat caataatact ataatattag tatatcattt
    26161 atatatttat gaaaaagata attatattcc tatcttgatt ctcttttgta aggttgtaaa
    26221 atcaaacttt ttataagtat aaaatctaga ttttttaaca taatctcaag gttcttatct
    26281 ttccatatat ctgacataga atttat
//
LOCUS       DAEOSP010000034        26270 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.034, whole genome shotgun sequence.
ACCESSION   DAEOSP010000034 DAEOSP010000000
VERSION     DAEOSP010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26270
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.034"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            300..698
                     /locus_tag="KRH54_001167"
     CDS             300..698
                     /locus_tag="KRH54_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8578836.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(915..1124)
                     /locus_tag="KRH54_001168"
     CDS             complement(915..1124)
                     /locus_tag="KRH54_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578837.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(1156..1365)
                     /locus_tag="KRH54_001169"
     CDS             complement(1156..1365)
                     /locus_tag="KRH54_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8578838.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            1664..2083
                     /locus_tag="KRH54_001170"
     CDS             1664..2083
                     /locus_tag="KRH54_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8578839.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            2311..2766
                     /locus_tag="KRH54_001171"
     CDS             2311..2766
                     /locus_tag="KRH54_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578840.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(2987..3247)
                     /locus_tag="KRH54_001172"
     CDS             complement(2987..3247)
                     /locus_tag="KRH54_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578841.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTGIKGMLQNGV"
     gene            complement(3510..3863)
                     /locus_tag="KRH54_001173"
     CDS             complement(3510..3863)
                     /locus_tag="KRH54_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578842.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLNQSSVNEASNISSFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(3880..9201)
                     /locus_tag="KRH54_001174"
     CDS             complement(3880..9201)
                     /locus_tag="KRH54_001174"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012632.1,HMM:NF024930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578843.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFTKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGHGNTMLIDNKGTLKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDIFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNKSASQYT
                     EIGVSNVTDAYSSYNATFIVADEKKVYATGINTNYLLGVNPNDGVNINLTLINNWFYV
                     NPSGASYSTNSCTNDITKINNIIIYEYMAILCTNYGSFLTGYHGTLWTKPSDSTYRVQ
                     QQGITHAGYISNYIYNYYPTRCTQSSYSTNFAYLYYGESTSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHVFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNVFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYICNDVSIIVKNDNTCYVCGFVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNVIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAESNEPQA"
     gene            complement(9218..10201)
                     /locus_tag="KRH54_001175"
     CDS             complement(9218..10201)
                     /locus_tag="KRH54_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBG8578844.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(10202..10900)
                     /locus_tag="KRH54_001176"
     CDS             complement(10202..10900)
                     /locus_tag="KRH54_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBG8578845.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMISREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(10897..11949)
                     /locus_tag="KRH54_001177"
     CDS             complement(10897..11949)
                     /locus_tag="KRH54_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBG8578846.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKMHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(11942..12370)
                     /locus_tag="KRH54_001178"
     CDS             complement(11942..12370)
                     /locus_tag="KRH54_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBG8578847.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDILSANVKVSTIYGEVEINV"
     gene            complement(12370..12696)
                     /locus_tag="KRH54_001179"
     CDS             complement(12370..12696)
                     /locus_tag="KRH54_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBG8578848.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(12712..14241)
                     /locus_tag="KRH54_001180"
     CDS             complement(12712..14241)
                     /locus_tag="KRH54_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBG8578849.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGIIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(14523..14945)
                     /locus_tag="KRH54_001181"
     CDS             complement(14523..14945)
                     /locus_tag="KRH54_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578850.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(14945..17398)
                     /locus_tag="KRH54_001182"
     CDS             complement(14945..17398)
                     /locus_tag="KRH54_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578851.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(17398..17565)
                     /locus_tag="KRH54_001183"
     CDS             complement(17398..17565)
                     /locus_tag="KRH54_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578852.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(17592..18038)
                     /locus_tag="KRH54_001184"
     CDS             complement(17592..18038)
                     /locus_tag="KRH54_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBG8578853.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(18152..18580)
                     /locus_tag="KRH54_001185"
     CDS             complement(18152..18580)
                     /locus_tag="KRH54_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBG8578854.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(18594..19658)
                     /locus_tag="KRH54_001186"
     CDS             complement(18594..19658)
                     /locus_tag="KRH54_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBG8578855.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(19663..20106)
                     /locus_tag="KRH54_001187"
     CDS             complement(19663..20106)
                     /locus_tag="KRH54_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578856.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMKEINLNLGE"
     gene            complement(20355..20795)
                     /locus_tag="KRH54_001188"
     CDS             complement(20355..20795)
                     /locus_tag="KRH54_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578857.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(20900..21097)
                     /locus_tag="KRH54_001189"
     CDS             complement(20900..21097)
                     /locus_tag="KRH54_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8578858.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            21266..21586
                     /locus_tag="KRH54_001190"
     CDS             21266..21586
                     /locus_tag="KRH54_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG8578859.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            21668..22174
                     /locus_tag="KRH54_001191"
     CDS             21668..22174
                     /locus_tag="KRH54_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578860.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            22875..23630
                     /locus_tag="KRH54_001192"
     CDS             22875..23630
                     /locus_tag="KRH54_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG8578861.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(23773..24201)
                     /locus_tag="KRH54_001193"
     CDS             complement(23773..24201)
                     /locus_tag="KRH54_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG8578862.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(24622..24822)
                     /locus_tag="KRH54_001194"
     CDS             complement(24622..24822)
                     /locus_tag="KRH54_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG8578863.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(25005..26174)
                     /locus_tag="KRH54_001195"
     CDS             complement(25005..26174)
                     /locus_tag="KRH54_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBG8578864.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
BASE COUNT         8422 a         4435 c         2804 g        10609 t
ORIGIN      
        1 ttaatctttt tctcttttac agacaatcaa aaagcaatct tagtttatcg aaataaattt
       61 atacaatcta ttcatacacc cctttacatt tatataacaa ttaaacctat tgttatatat
      121 acaaatcaaa ataaaccata tattttcaac tataatatgt taaaatcata ttatgagcat
      181 attaatttaa gcaaaatgtc atttagactt taatttagta gttttatatt ataaatttat
      241 atatttttaa ttttaaatgt catttagaca ttactcataa aaagaaagga gtgtattttg
      301 tggatacttt aggaaaaaga attgcttatt tgagaaactc caaaaaactt actcagcgca
      361 aacttatgga tattttaaaa tttgaaaact taggcaagta tgaaacagga gatagaaaac
      421 ctaattgtga tatactaatg tcaatagctg attattttaa tgttacgact gactggcttc
      481 tctatggtaa agagaaggta aatgtcaata gtagtgtaaa agaagataaa gaagattact
      541 tacatgttac taatgatgaa atgacgatat taaatctata cagacaacta aacgaacgtg
      601 ataaaataaa aatagaagga atacttgaac ttaagatatc tgaatataaa gacttaaaaa
      661 agcattcctc taataataat gaagataaaa tggtttaaac atacataatt tgatacgatt
      721 ttacatcatc tattaacctt ctttttttgt agtatattac taaaatatgg atattggatt
      781 atttaaaaac aagtatagtc taatttcaag cacatttata atatttagat tatcttatcc
      841 atattttagt ttaagtactt tgaaaaatat aatacatctc tatgtataac ttatattcct
      901 aagaaatgca ttactcatgt ttttaattct tccaacatct ttctatacac aattgtacat
      961 ccttcataat tcccctcttt aagaaggatt gaacatgtac tagaatattt agttaaagta
     1021 tttaatcttt ccatatttaa gctttttata ttactgagtg ctgtaataaa atcatcaatt
     1081 gctctacaac actctataac cccatctgaa tccaatttac acacaagaat attcacctac
     1141 tttatatact atttattaat catcttctga atattctata ttaagtaatt gataaattga
     1201 tgatttatat tttttacctg ttcttcttcc atttaaaata gctgacaaat agttttcatt
     1261 aacacctatt ttcttcgcta attccctttg agtcatattt aattcaataa gtctttgctt
     1321 gacttgcact cccaaaaaag gtatccattt ttttcgagca tccatttatc aacttccttt
     1381 ctaagtgatt tatatttact tttatattat tttagcttac acaccccctc tatttagtat
     1441 atatttttaa tatgtacaca tattaagtat ataaatttat ttaatactaa ataagatttt
     1501 ttgattactt tgtgctaaaa ttaggatgta aattcgtatc attaagttaa ttataaagtc
     1561 attttgacct taagtcaatg ctttttatgt cattttgacc ttaaatttga aactatttac
     1621 atcttaaaaa gtctttttga cattaattga atggagagtg tttatggata ctttaggaga
     1681 aagaattgtt tatttgagaa aagcaaaaaa ccttaaacaa tatgaactag aagaaatgtt
     1741 gggctgtgac aacttaagta aatttgagag aaacattaga aaaccaaatt atgaaatatt
     1801 aaaatctata gctgagatat ttaatgtttc agtagactgg ctattaaatg gagataatct
     1861 atcacataaa tcagatttaa tttgcgattc ctcctcaaac tatccactta atagcataaa
     1921 ctctaatgaa ataaagcttt taaacaattt tagaaagtta aatgattatg ataaagccaa
     1981 aatagaaggt atgattgaat taaaacttca tgaatatgaa aaagagaagg atttaggaaa
     2041 aattgagtat aacaaaaata aagatgagaa aatagataaa taatttttat gttgcttttg
     2101 atacgatttt acatcatttc tttgttctaa tatttttatt tcatttattt tcttttttat
     2161 aattttatga taaaataatt agtatatttt gctttacttg aatgtaatta tttatacaaa
     2221 agtcaaacat aatataaatg tagtaaattt ttagtttcaa gtaatatctc gttattataa
     2281 ctaaatattt attaaaatta ggaggataaa atgggcttaa gagacaaatt cgcgcaatca
     2341 tttgctagat caaaaactat gagtggccct gagaaaaagg ctaatgaaat aatgggtaaa
     2401 ttgcttctta agaaagctat attacctatc gttttaatgt tcgtgataat aatcgctgga
     2461 gctatgctta aaatcaattc ttgggttaca cttggcataa atttagttat tgctgttggt
     2521 gctttcttct acataagaaa ttctagtaaa aagtatcaga actttaaacc ttatgttggc
     2581 aatttgatta gtttagagaa aaaaggtaaa aaagagtacg ttgctattat aaaacaaggt
     2641 aagttaccag ttaagttaca aatagcttat ggtggtgaag accttgaaca tgttaagaaa
     2701 aatcaaatgg ttcaaataag ttataatcca gatgctaaaa tagctatttt ggttaataga
     2761 caataattta ataaaaaaaa ggagttgtct taaaattgat tttttctaat caaatttaag
     2821 ctcaactcct tttttaattt ttatatatta tttttatata ttatttttat tacttttttt
     2881 aattaatatt tttaaatatc aaatccttct caataaaata gggtactatc tacaatacta
     2941 ctttatcttt tcctaacaat atataatctg gtgctactct ttcatgttat acaccgtttt
     3001 gtaacatacc ttttatacca gtaacatctt ccttaatttc ctctacatct gttttcatta
     3061 tacccatttg aacaagtata tttttgttta tttcttgttg ttgtgtagat aattctataa
     3121 aattttctac agttttttta tacatgtctc tatcttcttt tttctcctgc atagtatttt
     3181 taaataatag agcacatagt atacctattg cccctagact actcaattcg gttattaatt
     3241 tatccattat agcctcctat tgtctaaatt ttttatactt atagctttaa tttttgttat
     3301 attcttacat catgcaatat tatattttca ggattatcaa tttaatcttc tgtactttaa
     3361 attgtaggtg caatttatag attgaataaa taataaatat atatacaatt tctaatataa
     3421 caaaaaataa gtacaaggct tacttattaa agttaagacc ttgtacttta cataaaaaaa
     3481 ataactaata catagttatt ctaaatttac tattcaactg gtatttctaa ttctttgcat
     3541 ctaattctaa cttgctctct aattactgct ggtacttctt ctattgtttt tttgcctctt
     3601 aaaattaaag ctacatatac ttctaccatt ccactctcac ctccagattt tcttacacta
     3661 aaagagctta tattacttgc ttcattgaca gaactttgat ttaacaacat ctcatatatt
     3721 tctgcatttg ctataaaatt atttgttata tcatcatctt gctttacttg acctatactt
     3781 atgtctgaaa tgtctttata ctgcatttct acttttctac cttgtttgat ttgttctatt
     3841 ttatttttat cgaatttaaa caattttcac acctccattt taagcttgtg gctcgttgct
     3901 ttcagcatca tatctaaaaa tcatagatgg acgttttata gctgtccatt tatctccttt
     3961 ttctactatg attttaggct gagcactttt tatgttggct tcttctaatt catagatttc
     4021 ttggtaactt gagtctgatt ttttgatttc aactagtttc atgtctttga ataagtcaga
     4081 tttatttatt tttacttgaa ctttagtttt atttattaat tgatatatag gattgttttc
     4141 catcatgtca tcctttaatc cctcatttaa tgttactatt aaatcagtgt tattttcagt
     4201 aatgtttatg attgaatata ctttattgtt tatcattaaa atagaatcct tttttacttt
     4261 aagccctgtt tctgatttaa atataaacat acttgaatat aaatcgcctg taggactact
     4321 ggctttaatc agtatcttat tttctcctaa tactatttta ttttgtggta tctcaaatat
     4381 aatgctattt atttccaaat tagtcttagt agatattaat tcatcattta taaacatttc
     4441 aagtttttct ataacattat tagggtcttg tactacaact ctcacttgag atattgattt
     4501 ttcaactatt tctatagatg ctttacttgt atctactttt gttatcatgt atattgtatc
     4561 atctgaaagc tctatatcta aaatatcatt tagatgttca ggaattggta ttttagtaaa
     4621 ttcatataaa tcagtaattg atatagatat tttatacata gatttatttt taccagttat
     4681 ccatagacta ttatcatttt taattactag tatgtggtca tcattatgtg acttaatttc
     4741 tttaacatta gttaatactt tagtaaaagt tcttattatc ttatcttttt cactttctcc
     4801 tagtccaaat aatccatttg tattgtaccc ttgagcgtac aactctccct cattagttaa
     4861 aacaagtgtc attgagttat atatacttat atctttaaca gaaggtaatc cagtaacctt
     4921 ttgtaactct cttacatcat tattgtggcc aactcccaat tggtactcag tattctttcc
     4981 atatgaccat agagtattat ctgtcttaat aatataggag tagttatctc cacaagcaac
     5041 ttttttgaca tctgatatat ttacttcagt aagagttaat aattctatat cttcaagggt
     5101 ttgattaatt tctttatatg aattacttcc tattccatat aacttgccat ccttagcaac
     5161 agccaatgaa tgtgtttttg atatatcaat atcagatacc tcaaatggca atattattga
     5221 agtatactta aaattatctt taaatatgtt tttataattt atatacttat tatttgtttt
     5281 tatatactta tcattaaaca ttactagctt ttcatttttc ttattaaaaa ctattttatc
     5341 tgatgtgtta tctgttttaa aatatgaatg tagggaacct tcaagagctt catttttatc
     5401 attatcatat ctatggtgaa tacctaaatg attatatcta ttatctcctg ttgaagaata
     5461 aaattttcca tatatatctt taataaatat agtattgtaa ctagaaaata cagtatcaac
     5521 atctttcttt atagatactt tataatatgg aactgacata tccctataag attcatcaga
     5581 tataccataa gtatctctac ctgatgatat caaattgtta ttattatcta tataaagtgt
     5641 attattagct gttacaaaaa catctttaac atcttccaca ctatttgtat tattattatt
     5701 tgctatactt ccatatccac taagacctcc aacaaatagt ttattgtcat ttgttaaaat
     5761 ggctgaagta tatccattaa ctactatttt ttttgcattt ataatattat taattactcg
     5821 tatacccttt atatcattag tttctgttga aaagaatacc ctctcttttt tccctgtagt
     5881 ataaataaca ttgtcatttg ttataaaaaa tgtagcttca cttcctgctg ttacaacaga
     5941 ttttacattc tcaatattta tttttgtaaa tgtacttata cttccttcag taaacccaaa
     6001 atatccattt acaaatccac atacataaca tgtattatca tttttaacaa ttattgatac
     6061 atcattacat atatacacat ctctaactcc atttataggt atacgtgtaa atatattcct
     6121 acttgtagta tcacccaatc ctaactgtcc ttttccatta ttacctgctc catataaaag
     6181 accatcatta gttaataata gagatgtgtt tccatataca gctacatatt ttacattttc
     6241 aacatttatt ttagtaaata catttctatt tacattatct cctattccta actgaccaga
     6301 attatttaaa ccaacagcat aacaggtatt atcatttttt atcgcaaata catgtttact
     6361 attgtcagta aatctattta catttataga ttttatatta tcaatattta ttttagtaaa
     6421 tacctttcta tttatctcat ctcccactcc taattgacct acatcattta aaccaacact
     6481 gtaaagcata ttattattag ttaataaata tgtagacttc gaatggctat atatttcttg
     6541 tatattagaa gatatttcac tactgctatt ctttttattg taatttatct tattatttaa
     6601 atttaaacta ctattaactg atataccatg cgtaccctta gagttatatc cacatataaa
     6661 taagctctta tcagttaata ttgcaaatct agttttatct ctacttaaat cataaggagg
     6721 tataaatata ttttttatat ttttcattac ctttttataa ttagtggcat tatatccatc
     6781 ttgtgtatct gaaacatcta gttcttttgc actttcagat atattcccaa aaccatataa
     6841 cgtgttagaa gataaataca atagagcttt atataaaaat atggctttat cagaggtaat
     6901 aggacctgaa tttatattag atgctgcaat attattagat gattgattgt taagataatt
     6961 ccttccatat tgatgtatat aagatgaacc tccagaaata agtaaattat ctggatttac
     7021 attttttaaa ttacttgttg attctccata atacaaatag gcaaaatttg tagaatatga
     7081 tgattgtgta caccttgtgg gataatagtt atatatgtaa ttactaatat atccagcatg
     7141 tgtaattcct tgttgttgaa ctctataagt agaatcactt ggctttgtcc ataaagtacc
     7201 atggtatcca gttaaaaaag aaccataatt tgtacataat atagccatat attcatatat
     7261 aataatatta ttaattttag taatatcatt tgtacaagaa tttgtactat aacttgctcc
     7321 tgaaggattt acataaaacc agttatttat taatgttaaa tttatattga ctccatcatt
     7381 aggattaaca cccaataaat agtttgtatt tataccagtt gcatatacct ttttttcgtc
     7441 agccacaata aatgttgcat tatatgaact atatgcatct gttacattgc taacaccaat
     7501 ctcagtatat tgacttgctg atttattact aagtccatta acataacttg aactctttcc
     7561 agaagtatat atttgcttat tattagttac aattaatgaa aaccagtttg ataaatcagt
     7621 atttataaca acttttttta cattatctat agatattttc ttaaatatat ctgaattaga
     7681 aggaagaaca cctgctattg agttactatt tggcccagaa taataacaag tgtcatcatt
     7741 tttaatgtag aatattcctc cagaaatatt ttttgatgct actatagact ttacattaga
     7801 gatacttaat tttgtcaact catttaattg acttggtaag tcagaaccca ttcctttgta
     7861 tctattattt cctagaccat atacatctcc attccctaaa ataaccagag tacaataagg
     7921 actaaaaacg acatctctta cagaatgtat atctagtgga agttcaatat cctttatttc
     7981 catttcttct tttaataaag ttaatttatc ctcagattta attaaattta tataattact
     8041 ataactatat ttttcactat ccattttctt aaattctaca atgtgcatat catcaaaaaa
     8101 cgaacctaat tgtccataag tattatctcc tgtacaatat gaatcattat ttgaatcaat
     8161 tagtatgcta tgtgataaac caatgaaaat attcttagct acaatatttt ttaaatctat
     8221 aaatgaatta tctatattct cagagtaatt tgctatacct aactgatata tatcattatt
     8281 tccagcaacc tttaaagtac ctttgttatc tataagcata gtgttaccat gaccacatgc
     8341 aatagttttt atatttactt cttctatctt agtaaatagt gatgattgaa gtgcatttcc
     8401 aaaacatttt tcctttccac aaccatatat atcattatta tacgtcatta acacagtgtg
     8461 ttcagaacca caagctatag tcttaacatc attaatagaa actaaactaa ataaatctct
     8521 attattctta tctcctaaac ctagttgacc ttgattatta aaaccagtag aatagcattt
     8581 attgtcattc tttattataa aagctgattt attatatgaa aatacagcct ttacattgtc
     8641 aacatctact tttgtaaatg tatttacatt tgcagatgct ccattagttc ctagacctaa
     8701 ttgaccatac aaattatttc cagcaacata taaagttcca tcattgtata aaagatatgt
     8761 tgattgaagt ccacatgcaa tatcctttac gccttttatt tctggaacct tctcaaatgt
     8821 agaaactgtt ttccctactt tttctaatcc caatcccatt tgaccatctg tatttatacc
     8881 tgcgacaagt aattctccat ctgaagtaag tataactgca tgagattctc cacatgcaac
     8941 ttttttaata ttggtagata tatttggtat tttcgtaaat aattttcttg ttgtattgtc
     9001 ttctcccaat ccaagttgaa agttactatt atctccacat ccatacaaga tattattatt
     9061 ttttagacca aatgtatagt tacaacatcc agtaaaaaaa ttaataaagt cataattatc
     9121 atacccttca tcaaattcat caactaccat atttttatcg ccaaagaaat tacctctttg
     9181 aagtagttta gttcttttca ttataaaaac ctcctaatta tcttatttca tacgaatatt
     9241 tatttaaagt tatatctgtc tgaccaattg aatatgccct attagatttt gtttcaacaa
     9301 agttattatc catttcatgt atatatctaa ctctatttgc attaaatggt agcaccacat
     9361 tcttaaatgt aacactacca agttctccag ttatacactt tctatttaaa tctaacttaa
     9421 ggccttctat agctattatg tttttagcat tagttaaggt atcataccaa tatccagctt
     9481 catctacatc tatttctgac tctaattcta agtatattaa gatatctaat actttttcag
     9541 ataatttatt gtaactattt tttaatacag atagttcagt ttgtatttga cctcctatta
     9601 catcatcaat agatatattt ttaatttttt ctactacctc atttatagct cctgataagt
     9661 ttttagcttc tgtagataag agttcttcgc caatttttac ttttatttct tttatatttt
     9721 tatcaaactt atcatctaaa tcttgaatat ccttttgagt tacaaaaacc aacgtcgggt
     9781 ctacttctaa tgtaacattt gaagtattgg acaattgtaa aatcattttt acaaccaaat
     9841 ctttaatact gccatcttca gcacgtggct tatacgtctc tggatactta cctatagcta
     9901 ttatattatt ctcattatcc agaacagcag cttctcttat cataaatcca ccaactgttc
     9961 ctggtaaaat agtctctata actatccaat taggattttt ttcatcaatt cttagagaat
    10021 ttatatttcc ttcccaaact acattcttta aagcagtttg ttcctctgtt ggctcataat
    10081 attctccatt actatctcca acttgaaact tggttaatcc aacaagctct cctagtgcag
    10141 ttgcatttgc tattttttct ctaccaattt tggttaatat tatataaaat ttttcttcca
    10201 attataccac ctccgattta ggataaacta ctacattttc tacacctgta tcatttgcaa
    10261 tagctatatt aatcttacaa ttattttcaa tgcatttaat gcttaaagga taaaccttta
    10321 tttcttctct acttatcatt ccagctccac agtttacaac agcactacat tttataattg
    10381 gttcaaagta tggatgaact tttacctctt cactacatac catacctcca ccacaataga
    10441 ccatactttt atctagtatt attggttcta aatagtgtaa taagtgagca ggttttactc
    10501 tttcaatcat atcgctacaa tctaaaagag ttttatagtc acagttattt gctataaaac
    10561 tcaatacgaa tgtaaattca tcactataaa cttttatatc tgtttctgat tttgtatatg
    10621 cctcacatat actttttata acttcaatag tactagtacc tctgcttctc attttagctt
    10681 ttatattgct acgtcttgtg tcaaaatcaa gttctttttt ttcaatgcag agtatgtctt
    10741 cccacatatc taatccccat gttgcagtgt caacaaataa ttggttaaag gtatcatcaa
    10801 tatcttcttt aagaatattt aattctgtat cgtatgcacc ttgtatctcc tctacaatgt
    10861 aatttctatc aaatgatggt agtttatcaa ttagcttcat tgattttcca cctcactaaa
    10921 attaacagtt gttacactag gtattttatc ttcattaata gttatattat ctgtacttcc
    10981 atttataagt aggttactta aatcgtgtac accagtagta tttataagta ttcccattac
    11041 ttttgtatag attatctctc ctctaatatc tctaaagtat gtatttatac tttctaggaa
    11101 agattctttt acattgtcta atgtatatcc atcttctagt ttcattactg cacttatact
    11161 tatttctatt ggtaatggtg tcacaactgt tatagttggt ccaataggct tctcttcatc
    11221 tatatgttgc tgacaccttt caattgtttc tgtatcaaca gcttgattat ttttcccaaa
    11281 tatcaagacc ttaactgttc ccggaccatc ccatcttgga taaaccttaa cattatagac
    11341 tccatctact tccaaagccc attcttcata gtgagcttta tttccacttg tagcttgatt
    11401 tctttggatt ttataaaatc tttcttttag ttcttcatct gtctctatat ctgtaccacc
    11461 ttcaaaactt cttgtgttag taatctttgt tactccagat atattatcaa ctagtttaaa
    11521 ttcacaattt gtaggtaaat tatatttctt accaacttcc agagcttgaa ctgggctatt
    11581 gtcaccttct tcactaccaa tagttacatc ttttattact acaaatagta aatctctata
    11641 tgatattatt gtgccattat ttattacagt acctttctct ccaataaatt caacttctcc
    11701 atttgactct gtacctaact ttctatatac accaaattca ttgactcttt tatcaagaaa
    11761 ctggttatat gtgtcttgta taaaagccat tttatgcatc tttgaaagtt ctagatatat
    11821 cttcgaaagt tctagattat ttccagatac catgttgttt agaaaagaac cttctccttt
    11881 ataaatatca agattaatat tttcaagagt tctatttttt attacttcat atgtctggtc
    11941 actatacatt tatttccacc tccccataga tagtggatac ttttacattt gcactcaata
    12001 tgtctctgtt aaagttagca ctttttacgt ttacttctaa tatatatgga tttattagaa
    12061 gggcctcttt tatgaatcta ctagcttcac tttctgtaag accttttgta tatttttgtc
    12121 ctattagttc tgaaagctct gtcccatatt ctaaagagta tatctcatgt tcatatctat
    12181 ttgtctttat acacttgtat atccaaactt ttaaggcttc ttttttttca attattttaa
    12241 agtcaccttt ttctaaaata ggttcatctt tttcaaaatc ccatgccact tcacgaaaga
    12301 ttggcaattc ttctgtttta ggtaagatat aatcctctgg gacacctata aaaggaaata
    12361 ttgtactcat tataagctca ccaacttact tacaacagca aatttttcgc ctattctaaa
    12421 cattattacc atatccccag attcaaagtt atctataaaa ggatttttta cttcatgctg
    12481 gtgttcttga tttgtttctg tgtcaaatgt ttctagctgt ctatcaatca tccaactatc
    12541 tatcaaaata tcttcttttt ctaatatgat gttatttgtc tctattttta aatctggtaa
    12601 tttactttta atttttccaa taaaaaaaga aggttgatta tgatactttc cttcttctct
    12661 tattattcct ataaattcat ttattggatt agccattata tcaccatcct tttataatac
    12721 tctcctagct gtgttaaaat ctcttctact acttaactta cttattttaa ctacatcacc
    12781 tttttgtgga gcatgtaaaa attctccatt acctatatat aatccaacat ggctgactgg
    12841 attatgaaaa aatactaaat ctcctgcttg aagatttttt tgttctactt tcttgccttt
    12901 tttagattgt tgatttgacg ttcttggcaa actaacatta acttttttaa aacaatagac
    12961 cattaaacca gaacagtcaa aactacttgg tccatttcca ccccatttat aaggtttacc
    13021 tagatgtttt tttgcttctg atattactat tttagcttta tcagatacat taccagaata
    13081 accaccaata ataatttttc cttttcttct tccaaattta ctagcttctt caacatttcc
    13141 aaataaaata tctatatggt atgttccatt cttttctata gttattgcag gtcctacgtc
    13201 atttacttta tagactccat cacgaccaga aactcctgta attacctgta tttcatctcc
    13261 atagtttaat aaaggatgtt tatttaaaaa ctcttttgta taataagttt gctcatattt
    13321 accaaccata ggagcagcgc aagttttttt agatgggtca agttttttct ttctacaatc
    13381 tgtatctcca ccttcttctt ttctaggaca gtaagctgta aattctgctg taaactcttt
    13441 tcctcctgca taatcttctc cccctaaatt actttcttcc ttaggttcat cctgtcctgc
    13501 tgacttttca tccataagat tttgaaaatt aagttcaagc tcaatttgat attctccatt
    13561 ttgccaagta tgtttgtctg tatctatata aaatagtcct acaagctttg tataagaatc
    13621 tttaactttt actcctctac cagttataca acttacatct ccataacctt taagagaaca
    13681 gcttttttct atcccattaa actcgctatc aatatctaca tcttgatttt cttgttgctg
    13741 aattactttt tgcattatta catttacttc cttaaaaatt tcattatcta ttttttcgct
    13801 aatcttgctt ccatactggt ctaccactat tactttattt tttacatttt ccatgctctc
    13861 cgaaaaggtg gtatttataa tattaaatcc ctcttcaaac ataacactta atgtaacagt
    13921 tcctttttca ataacattaa acttatctaa attggcctct atcatatact ttttctttgt
    13981 ctttttactt gcttctgtat atgcactcat tattgtgtca taaccattta ctcctataaa
    14041 catctttgta tacttgacat tggtctttgc tattattcca actgaaagcc tattttcagc
    14101 aaatacttgc tttgctatgt cttcaactaa cttatcttta aaattgtatg acacttcact
    14161 ttgtgtaagt aaaaatccca tatctttaga tacaaaacta atttcattac tgcttgaatc
    14221 tttagaccta ttaattatca ttcctctaaa gagttctttc tcatctacat agaaacagac
    14281 tgtactagct attggtatat cgatttgttg gaaatttaca tcaaatgatg attgtattat
    14341 tgaaaactct agtgtccttg atggagattt ataatcacct gaccatgata ccttgtctac
    14401 tatgtcagtt atgtcatata tacttccgtt ttttatgtgt acccaaatct taataataaa
    14461 aaatcacctg ccttttcatt taattctatc agcagttatt tataacttat tatctataat
    14521 agttattgtt ttggagttac ttttggtatc tttattcttt tatactcttt taaatctaat
    14581 gtaaaatata catctcgagt cccatctctt tcttcataac taaacccttc aattatgact
    14641 tccatattta tatttgtttc cgtaattgta aatcttaata taaaaccttc cttcatccat
    14701 ttttctatct tatttacaca atcatatggt gatggaaaac ctgtatagtc acagaaagta
    14761 tagtctttat ttggaaaaaa acttgatatt gaaatgcttt taagagctac accaccaaaa
    14821 gttgctattt cacctatttt gagtatacta gaagagttta cagcagcttt tccattaata
    14881 ctaaaggaag atggaaatac tggaaatcta aatctatctt cagcttgtct aagccacatt
    14941 tccattataa gacaccccct aactgtgcca attggatact tgcaactaat tttgatgtta
    15001 tcttttctat atcagcttct tctctaatca ctattgtatc agctaactta gctatgttta
    15061 tatttccacc attttttctg cttctatatt gatttgcttc ttgtttagtt aaaaccattt
    15121 caccttcatg gagtctggtt tgatagtcat tataaggaac atagttcaat ccaaatgcat
    15181 tattaccttt cttacctgaa taagctttac cagaatcacc agcctttgtt ccagcatatg
    15241 gattatcttt gtaatttttt ttactctttt ccactagatt taaaactgtt cctgtgatgc
    15301 ttccattttt tatcttatta atcatagttt gccaccattt acatatatct ttaactattt
    15361 tacatacttt atctaatatg ttaaaaaaag ctcccaatat tggctctata attttccatc
    15421 cagtttccaa cagaggtcct aaggtctccc atacagtttt ccaaataaca cccagtgtct
    15481 ctataaaatt gtttatttga ggtgcatatt cacctaaaaa agtaaaaatt cttgtgataa
    15541 taggactaac agcttcaact acaggtccga ttagttggaa tgctgtagtt actattgtac
    15601 caaatatact tattaatcct tgtgcaattg gtgatttatt gtttatacta tctagtaaat
    15661 tatttactaa gtttactact ggaagtaatg aatctattaa tgctgtaaaa tcgatatttt
    15721 gtaatagtgt acttcctgcg ttaacaattg cttttggtgc attccctatc attgttgctg
    15781 catgattttc aaaacgttct tgcttatttt caaccatcat tttacgcttt tcagcctcat
    15841 tagaaattcg atattgagta gaatcagaaa actcttttcc attttttaca cttctagatt
    15901 gcatctggtc atatttgttg ccaataatta tcttaccatt cattacattg cctattttac
    15961 cagccatttt ttcagataag ccagctaaat tgcctaatcc actttctata aaagttttta
    16021 ttttctttag tccttgacct aaaatatcat caaaaggttt aacaatactc ttcaagccat
    16081 tgttaatgct tgtttgagct cctgatatta tacctggtat tgtattactc atttctttag
    16141 ctaaccccat ctgctttaca cttatgcctt ccaatgcttc atcaaaagtt ttataatttt
    16201 ctcctaccag agtattaaga gattccatgt ttccttttgc tgcacttaag aaagctgctg
    16261 atacatcttg ttcactactt gtattcatat ttaaagctct aacatctacc atagcttgag
    16321 ttatatcttt ttcttttttg ccaccagtaa tattactaat cattttagct ttagttccca
    16381 ttgcaacaac atcttcaggg tcaaaaggcg tatcatttgc cattcttact aaactgccat
    16441 aataatcatc aacatctatt tgattaactt ttccagtatt tcttttcatt acactttcta
    16501 gaacaacttt ctgattttgt tcctgaccaa gtaaatcaac accctttttc acagcgacac
    16561 ctatagtagc accatttgtc aaatcagata tcttcccttt tatcgaacca agtattccac
    16621 ttgccatatc ctttacagct attgcaccac tccatacctt acctgcaaat aatctcaatt
    16681 tgggaaatac tttacttata atgccacttg ccatatcttt tatagctatt acaccactcc
    16741 aaaaagtatt tttaaacaac attaacttag gcaaaattct acccatagca ctactcacca
    16801 tatctttaag attaatactt ccttgataag gacgtttctc tacttgtttt aattttccca
    16861 agatactact tatcactctc ttagtcatat cttttgcttt tattacgata ttccaaatct
    16921 tgccaccaat cttttttaaa tttccaagta atttattttt agttttttct acttcatcac
    16981 ttttagcttt aaatattaat ataatcataa tatttgaatt aaactcattt ttcttttgaa
    17041 tcaacttatt aaaataatca ataatcatag ctatagttat tgctagtctc ataatacttc
    17101 catttaaact tccaataact cctgaaacag catttatgct agaacttaat tttgtcatag
    17161 ttgaacttac actactagca gtagtttgaa cttttcttaa cgaggaattt aaactgttta
    17221 ctctattact tgcacttcca atagaatttt ttatgctact agccattaca ttagtggaaa
    17281 gtgaaacagc cacagttgct ctcctaaaat tatcagttac tgatataatt tgattcattt
    17341 ttggtgtgta attatctgta aggtacatat ttataccttc ttctctcaca tttcccatta
    17401 cataccacct cctgtcaaag gacacactcc atatccatgt ttcatttttt caacttcttc
    17461 ctcaatttct aaggcgaaaa aagcttcaat aagctttaat tcgcctttat ccattgcata
    17521 aaaaagggac ggtcttatac cttttttctt ccaataatag tacattattg tagtcctggt
    17581 atccgtccct attagttttt tacctcttct atcactgcat ttttaccata acccataaca
    17641 tcttgaagta ttctatataa gttggtaatt tcacctggta ataataattt tcttgctaat
    17701 tcttttggtg ttggagcctt aaatttatcc attagctcct tatttttaaa taaaagcttt
    17761 ccatccaaat cacaaacacc ttctataatt gtaagcatct gcatctccgg taagtctata
    17821 tccgttttca tattttcatc aattttaaga caagtatttt gtatttcttc atctttactt
    17881 attgttaaag ctctacacat tactttaaat tcctgcccaa atatagttga taatctctta
    17941 agttcaacta tagtacttgg tctttctaag ttttctgtat ctgaatttaa aagtaaatct
    18001 actatgttta tattttttga taatccattt tcactcatta ttatttgctc ctttatcaat
    18061 caaattcaat caactaactt ttttacttat taactatctg aatttattaa gtatatactt
    18121 actaaatatc agtataaaac taaatataaa actaaattat atcaagaaaa tcatactcag
    18181 taaatgtgaa aggagcttct atctctcctt ttactccaac ctcccaatca gctaaagtta
    18241 aatcatcaaa tgctatattt tttactgcta ttctttctgc tccataagaa tctgggtcat
    18301 ttaattttga tatagcaaca aatctaggtt cagaacctct ctttatcttt tctccaatta
    18361 acttgtgcat tcttgaacta acgtggtata gagttattga gccttttcct ttatatccca
    18421 tatactttgt atcagtaccc atttgacctg ctattataat atcttctttt gtaaattcca
    18481 tctttgcttg aaatttcttt acttctgcta ctttgtttcc atcaagccaa agttctcccc
    18541 aagtaccact cattacattt ctagcttcca tatttgccat attaataatc ctcctatatt
    18601 tctattgata aatctatatc ttccatagca tcaagtactt ttatttttgc ttttaaaaat
    18661 actttagaac ctgtgttagc ttcttttatt tcttgtaatg tcatataaga taaatctact
    18721 ccttttgatt ttaaatacga tttttgtgct tcaaaatcta tttcaacagt agaatcagat
    18781 tctataagtg ctgatttttc taattcttct aaataacttt ttatagctac tatcaataaa
    18841 catttgttgt cataactgtt agtaaccttt cctatatagt catctattat cacctttctt
    18901 atgtcactat gtataatatc taaagtgtca actattttta ttttctggaa catttctcct
    18961 ttttctgctg ttaattcagt taaagaattt actcctctag caattcttat agctcctgct
    19021 tctttaataa gtataagctc tcctttatta acccttgatt ctgcatcaac tttcgtcatc
    19081 ttaggtatat cgactacatc actaagttta gtataagtta ctgattgact taaaggtgta
    19141 cctgctataa gtccagccac cctgcttgta aactcatcaa cactgtattt cttttctcca
    19201 actaacacat cttctgtagt aaaattaatt atcccttcat gatttcctac aacttttcct
    19261 aatacagctt taaccttaac cttatctata tctctaagtt taattatcca attttttata
    19321 gcagtcttat cagcttctac tgcttttggc atacatagat aattaaattc cttagtctct
    19381 aaaaaatcta atgcagtttg aatatctgct tctccttcta ttacataaac taataattta
    19441 tttggagtgt taacatttcc catcaaagct aaatttatat attctttatt ttcagcagat
    19501 aaatcaactg gtatatcctc tttttcatgt atttcattaa gacctagtgc cttagcatct
    19561 ttaagtacca ttgcaattat tcctctagct gaacgttctt taacagttgt agctagctcc
    19621 ttaaatgata tgttgatact tggtaatcct atagccatac tatcactctc ctaaatttaa
    19681 attgatttct ttcatatttt catatactac atcttcttca tatacactat ccagatagtt
    19741 tagtgtaaat gtaaagttca atacataaat tgactcttct tttttaatac tttgttctag
    19801 ctttgttata tttaattttc tgtctgttac ctttatattt ctgttaaata gcttatttaa
    19861 ttcatctgaa atttcataca ttctaatact ctttgactgt ggttttggta agtattttat
    19921 agagataaca atatctttgt tattcgtatt taacattaca ggtgtacaaa taactgatag
    19981 tatacttaca aaaaaacaag acccttcaga gccttgcaca ctttcatcat caataaaaat
    20041 actttcagta aagttttttt tgagaatctc aataattgtt tctaatattt ccttgtattt
    20101 aagcaaataa tcaccacact atcactaaat attcttgctt aatctgataa tcttactata
    20161 aatgatataa gtcattttta aatatatatc tcatttattc ctgtcaagta tcttctatat
    20221 ttttaggtaa tattattctt ttcaatctat cacctccttc aatatggatt tctttcacaa
    20281 taacattatc tcacatattt tattgcctat tgttgcaatg gtgtatataa agtgttgtaa
    20341 aattgtccta ttttttataa tatctcactc aatttaaata tcatcttttt ctttaaattg
    20401 tagcaagatg ttctatctaa gtgtagtttc atagaaatat atgtcatatt gttttttgtc
    20461 ttactattat aaaaaagatt aaaaaattcc atttctatat catttagaca cgttagagca
    20521 ttttctatct tctctttttc tatttctata tcttcttttt ccatttttaa ttttcttaat
    20581 ctttcctctc tttttaacac ctcattttcc acagaagagc ttatgttata tgtgttacta
    20641 gtcttttctg tatataccat tgctccacat ccagagtatt cattctcaca tctttttatg
    20701 tccaagttaa tattctttat ctttatatcc aaatacttat aattatgtaa tcttttatct
    20761 gtttctttaa ataatatatc ttttttgttt tccatagtac ttcccctttt ttatatactt
    20821 attattagct tcaaatttac aataatctct ttagtgcaca gttttttact aatctcacaa
    20881 ctttcaagaa catattgttc tataactgta taaaaaaaac ttcttctata ttttgttcta
    20941 aaatactaga aatcttctta gccataagaa gtccaggatt ttttatacct ctttctattt
    21001 gagatatata atcatttgta attccagcct ttctggcaag ttgaatttgt gtcatacctt
    21061 tgcattttct ataatatttc aagttatttt taaacattgt tttatacctc cattactatt
    21121 aagaatattt tgttcttcct cacttagatt atatagaatt atttgttctt tatcaataga
    21181 ttttagaaaa aaatattctt ttatatttta ttagttttat tttttagaac aatttgttat
    21241 aatctataca taaggtggtg ttataatgtt tgccaaaaga ttaagagaac ttagaaagga
    21301 atttggattg actcaaagag aacttggaga aaaagtaggc gtttcccaaa gagtcttagg
    21361 gtactatgaa acagaaaaca gatttcctga tgaacacatt ttaaataaat tggctgatgt
    21421 atttaatgta tcagttgatt accttcttgg aagaacctta gttaaggaaa atattgatac
    21481 agtagctgca catagaaaaa atccacatga agagttacct gaagaagcac aagaacaact
    21541 taatgattat atagaatttt tactaaataa atataaaaaa aaataaacca tatgatgagc
    21601 agtctatttt tctgctcttt taatataatt taaaggcaaa catacattct attttagggg
    21661 gatttttatg aataatttgg ataagctttt tgaattagct tctcaagaag agataataat
    21721 tcattatacc acttatattg caggtgattt agaaggacta tatataaaca aacatggtat
    21781 taaaattata tcattactca gtaatttaaa acaaaactca aaaaaattga catccatctt
    21841 agctgaagaa cttggacatc atttcactag ccttggatac tatgtatcct cttataacga
    21901 ttattataca aaaattatta tagataagtg cgaaaataaa gccttaaaat gggcttgtga
    21961 gttcttaatt actgaagaag atataataaa cataattaac tctggcatca cttgcgttta
    22021 tgaaatggca gacatactta atgtggatat tactttcttt caaaaaagat tagaatttct
    22081 atcattaaaa aaacagtctt tacaacttgg aaataataaa tatttaatat taactaattt
    22141 gccgtatttc tacatatttg atccaatttc ataataaaaa tagtagtaat tttaactggc
    22201 ttaacagtaa aatctatata tttattttaa caaattttgg tttctataaa ttcaatatta
    22261 atatataata atgatatcat attatttaaa attctaaaaa caaatatatg cttttgttaa
    22321 gaaaggttgt ttgatgaatg tataaagaaa agttgtttga taattatttt aagttcttag
    22381 cactattatt ttggccaatt atgtggtata agtggatagt aatttcaaat ggtactcttg
    22441 aaaatatgtt atttaccata tatgcaatcg tcgcaattgt tttcatcatt ttatattcgg
    22501 tgtttatgat aaaatataaa gacattacac aaatagattt cttttacagg ataagtactc
    22561 tacttgcttt tatatttaca ctttttagct ttttaatata cccaaagagt ttatttttct
    22621 tgtatttaaa gattattttt actggaatat acctttatta ttcaatagta aaaactctta
    22681 aatttaaaga tgacgaaggt gtagtgggta ttatgagctc acttttatta attgtaataa
    22741 ctttgttcta ctaaatttaa ttctatttga cttaatatgg ctttaaatat tagacatcta
    22801 cattattttc tgatattatt taagatggta gtcttatatt aataatgaaa tacactatga
    22861 aggagttaac tattatggaa aaaaaagttt tagcaacagt tggagaaaaa gagataacta
    22921 atattgatat tgaaaatgca ttaaaatcat tagaccctta tcaagctatg caatttaaaa
    22981 ctgaagaagg aaaaaaacac ttattaaatg atttagtgaa tcaagaatta ttcttcttag
    23041 atgctaaaga agaaaaatta gatgaagaag aaatatttaa attagaaatg aaaaagatag
    23101 aagaaaatgt attaaagcaa tttgctataa ataaagtttt atctagtgtt aatgtaacag
    23161 aagatgaaaa agtaaaattc tttgaagcta ataagagctc tttctctaaa ccagaaagtg
    23221 caactgctaa acatatatta gtagatagtg atgaaaaagc taaagaaata cttgctcaaa
    23281 taaaatcaga agaaatatct tttgaagatg cagctcttaa acattcttct tgcccatcta
    23341 aagatatggg tggtgattta ggaacattcg gtagaggaca aatggttcct gaatttgaag
    23401 aagctgtgtt ctctatggct aaaggtgaag taagtgaacc agttaaaact caatttggat
    23461 accatataat aaaattagaa gaccttcaag aaagtacaga gtctacattt gatgaagtta
    23521 aggctgaagt tgaaaaaagt ttattatatc aaaaacaaaa tgaagtttat ggaaataaga
    23581 taaatgctct taatgctaag tacggaaacc ttgtaaaata caacgattaa tcataaggtc
    23641 atatataaaa aagctgtctt ttcacaatat ttattagaaa agacagcttt ttatattaaa
    23701 gtttaaatat tcagtttcat attatcattt ttttattgta aagaactcag cttaaaagtt
    23761 taaataagat ttttaattat cattagctaa atttttatat ccacgtatta tctcataaca
    23821 gtaacctatt acatctcttc tttttattat cccaataaat gtgtcttggt catctataac
    23881 aggaatgaaa ttttgattta aagattttat aactaaatct tcaatatctg cgttgatgga
    23941 aacaggagaa ttatccattt ttctatttat atctgtcact ggaacatctt ccaatccctt
    24001 taagtccaga tttaaatcat tttttaatgt ccataaaaaa tcaccttctg ttatagttcc
    24061 aacatattta ccatccttac ttattattgg aattgcagag tatttgtgat attccatttt
    24121 ttctagggct tgccttatag tgtagtcttc atatatatat gctacttcac tttttggtgt
    24181 tagaaagaat aatatgttca ttacactacc caccgttact gaaaacttat aaatattact
    24241 caatatttat aatgttactt ttttgcagta actttctcct ttcttatttt ctaagtattc
    24301 ttaatactat acgctgtaac aagcgttttt cctttgctca ttttaatctt attatgcttt
    24361 attaatattt ttatatggtc tataagtatc acttattctc tttttagaaa atttactaaa
    24421 aattaattga cttaaactta gccataataa ttttactcta cactattaat tttaacatat
    24481 ttatctcatt attaatgatt ttattttaat aattttaatt tattttgcta ttaattttat
    24541 ttacaaaaac tgaaaaaacc gctaaatgat tagcggctta tctctcatat tactagaaca
    24601 ctaatatata tgaattttta attataatct aactacgtta gcagcttgag gacctttagc
    24661 tccatctact acttcgaact caacgctttg tccttcttct aatgttttgt atccatctcc
    24721 agttatagct gagaaatgta cgaaaacatc tccttcacct tctacagata tgaatccaaa
    24781 tcctttttcg ttgttaaacc atttaactac accagttttc attaataaat cctcctaaaa
    24841 aaacacaaat atttagctac tcttttctgt aaccttatta ctactctatc atatatagct
    24901 atgtttgtca attattatta tttttataca ttaataatca atttttttac tttataattt
    24961 taaatgatta ttatctttta cactttattt ttctttataa ttttttaata atttattttt
    25021 attatatgga atcttatgga cttatcactc cagcctaaat cccatggcac caacattcta
    25081 tcagtattat ggcaagttac caaaggatat cctcttgagt ctaatcctgt cactgtcgaa
    25141 acatgtgtta ttctgccttt cttctcataa ccaacaaaat cccctggacg taaattataa
    25201 gcttctttgt atacttcttc ataggaacct ttagcaatta atgaacctct tccactatac
    25261 agtgcataat ttttaaaagc ttgtgcattt acccatgctt ttgtacctgc tctctcgcta
    25321 taattccaaa ttgagttctt tttgaacctt ccactttcaa acattatctg tgatgcaaag
    25381 tttgcacaat ctccaccttc tggattataa tccctatagt ttggattaaa tttcatacca
    25441 tgttcttcat cagctgcggc tccacagtat ttatgtgcat aatctatagc ttttttttgt
    25501 tcatctgtaa gctgtaactc tacaccatct tgttgaccaa tataatttct tatatcatca
    25561 gattttatat tttctaaatt taaagagtct gcaaaagggt ctgtatacca ctctttagtt
    25621 atgacatatt gattatctat aatttttaca tttagataat gatatgtacc tattctaaag
    25681 gcattttctt tgtccttttg attttcatat gaatacttat attcagtgga tactgaacag
    25741 attataccat agaggtcttt ttctttttcc tttatttttt taatcttaac ccttgtcttt
    25801 atgtctttaa attttacacc ttgtttacta gaccagtttt ctaaatattt catctttttt
    25861 acttcatttt cataagccca cagaccaaat tttttatccg tttcataaag accttttaaa
    25921 atattttcat ttttctctaa tattgcctta tttctgtagt caaataagtt ttcaagcaat
    25981 agtttatatt ggtcttttac atcatcacct tcaacatttg cagattccat gactttttct
    26041 tccatatttt tcatacttaa atcattgatt aaaaatacac ctgctgtagt agctaaagac
    26101 aaaaatacaa atgccattat acctttgatg tacttattct tatttttcag tattttcaaa
    26161 gctacacctc ccaaatagta caccttacaa atcaaattac aaagaatatt ctgtcacata
    26221 tagtaatttt catatttaat aattctctat tttattatgt ccaatattaa
//
LOCUS       DAEOSP010000035        26182 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.035, whole genome shotgun sequence.
ACCESSION   DAEOSP010000035 DAEOSP010000000
VERSION     DAEOSP010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26182
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.035"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(47..934)
                     /locus_tag="KRH54_001196"
     CDS             complement(47..934)
                     /locus_tag="KRH54_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG8578865.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            1053..2252
                     /locus_tag="KRH54_001197"
     CDS             1053..2252
                     /locus_tag="KRH54_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBG8578866.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            complement(2446..5001)
                     /locus_tag="KRH54_001198"
     CDS             complement(2446..5001)
                     /locus_tag="KRH54_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG8578867.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKEYTVDRLGITKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIEVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(4998..5684)
                     /locus_tag="KRH54_001199"
     CDS             complement(4998..5684)
                     /locus_tag="KRH54_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8578868.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            complement(5761..6669)
                     /locus_tag="KRH54_001200"
     CDS             complement(5761..6669)
                     /locus_tag="KRH54_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8578869.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            complement(6671..7342)
                     /locus_tag="KRH54_001201"
     CDS             complement(6671..7342)
                     /locus_tag="KRH54_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8578870.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGYGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            7604..8668
                     /locus_tag="KRH54_001202"
     CDS             7604..8668
                     /locus_tag="KRH54_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HBG8578871.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            8826..9512
                     /locus_tag="KRH54_001203"
     CDS             8826..9512
                     /locus_tag="KRH54_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8578872.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKGNYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKVKAFLNHLTIKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            9502..10533
                     /locus_tag="KRH54_001204"
     CDS             9502..10533
                     /locus_tag="KRH54_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8578873.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITADLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIRVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            10615..11280
                     /locus_tag="KRH54_001205"
     CDS             10615..11280
                     /locus_tag="KRH54_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8578874.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            11293..13710
                     /locus_tag="KRH54_001206"
     CDS             11293..13710
                     /locus_tag="KRH54_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8578875.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKNKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTTIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNLVQMYAKLKSLGMTSFQLKKIIFLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKVKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKLIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTEGMFFASDKTLESLTPYNTN
                     LDFSILIDKKYSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(13891..14682)
                     /locus_tag="KRH54_001207"
     CDS             complement(13891..14682)
                     /locus_tag="KRH54_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG8578876.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(14715..15302)
                     /locus_tag="KRH54_001208"
     CDS             complement(14715..15302)
                     /locus_tag="KRH54_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8578877.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            complement(15478..16869)
                     /locus_tag="KRH54_001209"
     CDS             complement(15478..16869)
                     /locus_tag="KRH54_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBG8578878.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSREIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKIGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(17059..18357)
                     /locus_tag="KRH54_001210"
     CDS             complement(17059..18357)
                     /locus_tag="KRH54_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBG8578879.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKDIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNN"
     gene            complement(18716..19591)
                     /locus_tag="KRH54_001211"
     CDS             complement(18716..19591)
                     /locus_tag="KRH54_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBG8578880.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(19784..20470)
                     /locus_tag="KRH54_001212"
     CDS             complement(19784..20470)
                     /locus_tag="KRH54_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBG8578881.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(20519..21052)
                     /locus_tag="KRH54_001213"
     CDS             complement(20519..21052)
                     /locus_tag="KRH54_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG8578882.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(21096..21728)
                     /locus_tag="KRH54_001214"
     CDS             complement(21096..21728)
                     /locus_tag="KRH54_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBG8578883.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(21818..22897)
                     /locus_tag="KRH54_001215"
     CDS             complement(21818..22897)
                     /locus_tag="KRH54_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBG8578884.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(23030..24376)
                     /gene="pepV"
                     /locus_tag="KRH54_001216"
     CDS             complement(23030..24376)
                     /gene="pepV"
                     /locus_tag="KRH54_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBG8578885.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(24445..25725)
                     /locus_tag="KRH54_001217"
     CDS             complement(24445..25725)
                     /locus_tag="KRH54_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBG8578886.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKSKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(25852..26112)
                     /gene="hfq"
                     /locus_tag="KRH54_001218"
     CDS             complement(25852..26112)
                     /gene="hfq"
                     /locus_tag="KRH54_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBG8578887.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
BASE COUNT         9068 a         4053 c         3105 g         9956 t
ORIGIN      
        1 tatctctatt tttatattaa attacttgtg aaatatgtat tctaaattat atattattag
       61 atatcatgct taaaaattct ttggttgcta aggataaaga tatatctttt aaatggcagt
      121 aacctatatt tctcttaggt attggctctt ttatatctaa tttaaacaat ttctcatttt
      181 ccaaataatc tatgctaaat tcttctataa cacaagacac tccaagattt atatacgcaa
      241 attctaaaag taattcatga gaacccaatt ctatatccgg gtttagtttt ataccttttg
      301 ataaaaaata ttcatttaca taaagtcttg aatttgcttt attttctagc attattaaag
      361 gcaatgtgtt taattcttcc aatgatattt tcctaccttt gtattctatg taatcatttg
      421 cacatacaaa tatatcatgt atttcaatac actcaacaat atttagtgta tcatcttcta
      481 ttggcatgtt tataattgct atatctatat tcccatcttt gagtaaagat ataagttctc
      541 tactcgttct atttattgta gatacatgta tatgtggata cttcttatga aaaatttcta
      601 aatattttaa caagaaaaaa cgtgcagctg tatctcctac tcctattttc agagaaccgt
      661 attctaattt tttaaattta tttattctat cttctcctga tgatattaac tttaatgcag
      721 tagtgacatg ttcacttaga attttcccct ctggagttaa ttttactccc ttattgtttc
      781 tataaaataa taaagtatct aattgttctt ctaattgctt tattgactgg cttacagctg
      841 gctgtgacat aaacaattcc ttagcagcaa gagaaaaact cttattattt acaactgcat
      901 taaaaacctt ataaagttca agttttatat tcatatataa ttactcctta tgtcaaatat
      961 tagtattatt tattttactt ataattatat ttgaattata atatacatgg tactaatatg
     1021 acaatattaa ttctttagga ggattttaga taatggatag attagttgga actgtttcaa
     1081 gaggggttag agcacctatt ataagacaag gagatgactt agttaaaata gttgttgatt
     1141 ctgttttaaa tgcgtcaaaa agtgaaaact ttgaagttag ggacaaagat gtaattgccg
     1201 ttacagaagc tgttgtagct agagcacagg gaaattatgc tcacgttgac aatattgcaa
     1261 aagatgtaaa agataaattt ggagatgata ctgttggagt tatattccca attttaagta
     1321 gaaatcgttt tgctatatgt ttaaaaggaa ttgcaaaagg atgtagaaaa gtcgtattaa
     1381 tgttaagcta cccttctgat gaagttggaa atcacttaat aagtattgat gaacttgatg
     1441 ataagggaat aaatccttgg tcagatgttt taacagaaga aaaatacaga gaactatttg
     1501 gatataacaa gcatacattt actggtgtag attatgttga ttattataag aatttaatca
     1561 aggattgtgg tgcagaggta gaaatagtat ttgcaaacaa tccaaaaact attcttaact
     1621 atacaacaag tattttaact tgtgatatac atacaagaca aagaacaaag agaattttaa
     1681 gacaaaatgg agctaaaaaa gtatattcac ttgatgatat aatgacagca agtgtagatg
     1741 gtagtggtta caatgaccag tatggacttt taggttcaaa caaagctaca gaagaaacag
     1801 taaaattatt ccctataaat tgtgatgaag ttgtaaacaa aatccaaggt aatataaaag
     1861 aaataacagg gaaagatgta gaagtaatgg tttatggaga tggagctttt aaagaccctg
     1921 taggtaaaat atgggaatta gctgacccag tagtatctcc tgcttataca aaagggctag
     1981 agggaactcc aaacgaagta aaattaaaat acttagcaga taatgatttt gctgacttat
     2041 ctggtgatga gttaaaagaa gcaatttcaa aatacatagt tgaaaaagat aataaatctg
     2101 atgatttaac aggaaacatg gtttctcaag gtactactcc aagaagatta acagacctta
     2161 taggttcttt agcagattta acatctggaa gtggagataa gggtactcct attatttaca
     2221 tacaaggata tttcgataac tatacaaaat agtatgtttt aataagattt aaattttttg
     2281 gattatatat attaacatga aaatttatta aaacctctta atagatatga cttttcaaga
     2341 gttatatcta ttaagaggtt tatttaatgt tcacagataa gatatattta aataatttaa
     2401 acaagattgt cagaaagtta tataatctta aaaatttata taactctaat tcttatttat
     2461 ttctgaaata atatcactct tcaaaatttg tcttactgga ataattacag caactatact
     2521 aatagtgata tttaaaataa tcatacctat gtatagaggt gtatttattt taagaattgg
     2581 atttatatat aagtatacac gttttaccat aaaataaatc ataaattgtc caataacact
     2641 tccaattacc attataatac ttgcatagaa tccatataaa aaaccttctc ttatcatcat
     2701 aagtaaaaac ttattatccg taatacccat agctcttaaa attccaaact catgtcttct
     2761 tgaaaaaatt aagtagctta ctgtattgat tatatggaac aaacttattg caagaagtac
     2821 aaatacaata ccatatataa ataagagttt ttgatttaag taggtattat ttttttcaat
     2881 agcaatggta taatctgtta caatacatct ttttacatca cttacaacac tttttatgtt
     2941 ctcagacaat tcctcactgt tactatcttc tgtctttttt atacttactg aattataatt
     3001 ttcgatatca aagttatttt tcatttgccc attcgtcata actatatcca taccataatc
     3061 accaatgaaa tatatgttat ttgccatcac acgttttaca gtagcagcaa ctacaaattc
     3121 ttttgttgta taatcattct tattttcttc tttaaattta acaatttcat ctgttggttt
     3181 cattgtcttt ggcactttaa cttttattgt atctcctgga tttatatcaa ctgcatcata
     3241 cataccagtt ccatccatag gtagtctaac tataacctta ttatttttaa ccatatcatg
     3301 ggtattaatc tccccatcta taatataaga atttaatcct tctaacatac cattgttata
     3361 accataaata tttgttttta aaagatagcc accttcattt tgctttgtac aaataccatc
     3421 aaaaaaagtc tttatcctgt aatctttttc aagaggttta aagaaatttt tccaaaatag
     3481 ttgttcttct ttaatttgca tagctccaaa taaatatctt actggataga cttttgatat
     3541 tccctcaaca ttgtttaatc ttgaaatttt atcttttggt atacctacat caaaatctgt
     3601 tgtttgcata gttatttggt agtcagaatt taaaccatca tcagctttca ttgtaagttc
     3661 attattattt ttagtaagct gtatagagta actagaacat agaaatataa ttccaccaag
     3721 tgataatgaa aacaaaattc caataaacat tccctttttt tgagtcatgt atttatgaga
     3781 taatactttt gtcatgttag agtgttttac actttctaat attctctttt tcttattagt
     3841 aatcttaaag tttttcatag attctatact agagtattta ttcaactgat gtacaacacg
     3901 tacagcaatt aaaattaaca acaataagat aaataaaaag cctaaataaa tagatttttc
     3961 acttataaaa aatgctcctg gaacaatatc actactcaaa aatatatgtg gaaattgttc
     4021 atatattgta gacgccactt caatacctaa gaagcaacct ataggaaatc caacaataaa
     4081 aagtaaaaat aactctaaaa atagtagaaa taaaagttgt ttattattag cgcctaatac
     4141 ttctaaaacc ccatattgtg atattctctg aagtattgaa acatggaaaa tactatatac
     4201 tgctgctgca ccaaacactc caacaagtaa tataattgca tttgtattta gttctaaatt
     4261 ttttaagaac tcaaatatat tatatttttc tatatttttt ggtggtttta ctccaaattc
     4321 aactgaaaga ggccagttta tataagtttt ttcttttgat atgttgaatt ttttcataaa
     4381 tttataattt tgctttttaa tatttttatt ttcatcatat tttacataaa ctatataaga
     4441 atccttttct ttaggctgaa aataatcgtt tacaaatccc tccagagcag tttctgggtt
     4501 tgaaagactt gataaaatac cacataactt atagatttta ccatctaaat tgatttctgt
     4561 tccaatttct ttgtttacat ttaaattgtt tagagaatat gtgtcaagta ctatctctcc
     4621 atctttacta ggaagagttc cttttaataa ttttctacct gtcatgtcta aatatgaaga
     4681 atcagcattt aaaaattcta taataaaagg ttcatctatc tgatttttag gttttgtaat
     4741 gccaagtcta tctacagtgt attcctttga ttttttattt tctttaattt tattaagttg
     4801 ttccttatct actttataga aataatgata atctccacta tcttccctta tcttttcaat
     4861 tctacttttg tctgcactat gaactaaaga accaattccc gtcaataatg ctactgatag
     4921 tactatgctt aaaactatag atatagattg ttttttataa tgctttatat attgaattga
     4981 aaattttact agagacatca tagtacttca ccaccagtat tcaccacaac tccatcttca
     5041 atatggataa ttgagtcgca tacttgtgct atattttcat tgtgtgttac cataagtatc
     5101 gtttgatgga attttgcact actttctttt aataaacata ccacttccat tgtagtttta
     5161 ctatcgagat ttcctgttgg ttcatctgct aatataagcg caggtttatt tgccaaagct
     5221 ctagcaattg ctaccctctg ttgttgacca ccagatagtt cgcttggaaa ttttaaacgc
     5281 ttatcccaaa ttcccaatgt cttcatcagc atttcaatat attcatggtc tatatggttt
     5341 cctttatcta atgtaacagg aagagctata ttatcataag tgtttaatac gggcattaaa
     5401 ctgtagtttt gaaatataaa tcctatattt cttcttctaa atactgtcaa atcttttttt
     5461 cctattttag atatgttctt tcctttaatt attacatcac ctgaagttgg attatctaat
     5521 ccacctataa tatttaacaa tgtgcttttt ccagaaccag aagttccaac tatagcagta
     5581 aatgtatctc gttcaatttc tatacttact ccatttaagg cttttacttt agtctcacct
     5641 tttccatagt gttttactaa attattcgtc actaatatac tcatttaatt tctcctttca
     5701 atacagatat atgttctata aaatattgta agtgacgata attacactca tcctacaaaa
     5761 ttagtttaaa ctcttacaat tctgtaagaa gatactaaaa caacatccac ttcctagttt
     5821 agattctact ataatgtttc cattttcttt ttctaaaatt aatcttgtaa gatatagacc
     5881 aatccctgtg ccattttggt tttccacatt tttacttctg taaaatcttt taaaaatatc
     5941 atttagttct ctactatcaa tacctattcc ctcatcttta atagtaatct tcgtatatag
     6001 ttccattttt attacagaaa tagtaatcgt gctatctgat ggtgagtatt ttattgcatt
     6061 ttctaaaaca tttacaattg cttctatggt ccacttttta ttatgaacta actttatgtc
     6121 tgaaaatggt tcgagattta tccttatatt tttagcttct gctttattaa agatagtact
     6181 gatactttga ataagagtat ctttaattag tagttcttct acttcaaatt ctataactcc
     6241 atcctctaat ttagtcattt taaataagga ttgtaacagc cagtcaatct taaaagtttc
     6301 atttttcatc ttttgtaaaa actccttctt ctgtaatgtg gaaatattta tattctccag
     6361 aagttcacaa taaatggtta tatttgaaag tggagttttt aattgatgtg acatatttga
     6421 tattaaacct ttaatcgctt ctttttctga ctttgactgt tctacctcta tttctatcat
     6481 atccttaatt ttttttactt ggtgagaaat ttttgacgct cttgtatcct ttgtatctgg
     6541 ataagtaact tctttacctt ccaatatttg atttaacatc aaatttaatt catcatatct
     6601 ttttcgattt attttagtag atactataaa tataacaact gtacatatta taaaggctaa
     6661 tataaacata ttattctacc tcattccata aataacccat tccataagct gtttttatat
     6721 attttggatt agatgggtca tcctctactt ttactctaag tcttcttata tttacagata
     6781 caatattatc atcaacataa ttttcttcgc tgtcccaaat atggtgtaaa atttgttcct
     6841 ttaataatat ttgaccttta ttttcaatta gataacttag aagcttatat tctacagagc
     6901 taagttccaa acattcttta tttttatata cttttagtaa cttttggtcc aattttattc
     6961 cattggaata tattacttta ttattcgatt tttttcttat tgctactttg actcttgcct
     7021 ttaatacaga taaagaaaat ggttttgtaa tataatcatc aagccctatt tctaatcctt
     7081 ttacttcatc aatttctgag tctcttgcag taagcattat aattggtata tctgactttt
     7141 cccttattat ttgacataca tcaaatccat atccatctgg aagattgcag tctaagatta
     7201 taaaatctat atttttacta ttaaatatat ttaatccttc ttgttttgta taagccttaa
     7261 atacattaaa tccatcattc tcaaatgcat aaaaaagacc ttcatttaag tttatatcat
     7321 cttcaataat taataagttc aattttactc tcctttagat tttttagata aataattatt
     7381 ttcttgcatt atcatactaa aaatatttta attttattta tttcactaaa aagattgtat
     7441 ttaaatttgt tttcaaaata aaaacatcat aatcaagcat atagttatat atttcccaat
     7501 ttaattacgc ttaaattgaa aatataactt tattcttata taattaaatt atacaacaat
     7561 aaagttatat tttttaaata tatttttact agggggaata gaattgaaaa gggttgatga
     7621 gagaaatact atgtttgcaa gagcaaatta caaaaaagat agtactgctt ataatgatta
     7681 ttataagaaa aatcctgaca aaaaagaaat tgatgatagt ataagaaata gaccaaattt
     7741 atgtagtgaa ggaacaatga cttacaatga gttaaactct ccaatggcaa gtagtgcatt
     7801 cgatttttta tctgatatca aatctttatg cgaaggaaaa gtttctgata ctaaagtaga
     7861 tgtagatgca aagtcaatga caaaaaaaat taagggtctt gcaaagcagt atggagcttc
     7921 tgtagttggt ataactaaat taaaagacta tcatatttat acaaataggg gtagacatga
     7981 agaaaattac ggtgaagaaa ttaatctaac tcataagtat gcaattgtat ttggatgtga
     8041 gatggataaa gaaatgataa atagagctcc aatgatatgt gaagttatag aaacatcaaa
     8101 gtgctatgtg gatgcatcta tcgtcggcat gattttaagt tactatataa gaaatttagg
     8161 atatgatgct agaaatcata tggatgctaa ttatttggta atgcctgttt ttatagctcg
     8221 tgatgctggt ttaggagata taggaagaaa tgctatactt acgaataaag attatggctc
     8281 tagacttaga ttaggtgttg ttactacaga tattcctctt ttagaagatg aatatgttga
     8341 ttttggacta gaagattttt gtaaggtctg taaaaaatgc tcttttaact gcccttctca
     8401 ttcgctttct aatgatatta aaattggtga tgatggaaaa tataattggg taattgaaca
     8461 tgagacatgc tatattaaat ggagatattt aggtacagat tgtggtatgt gtatatctgc
     8521 ttgcccattt agccaaaatc tcgaaactat aaagaatact acttcattta agggtaataa
     8581 tgagcttatt caaaaagctt tagatgagta tacttcaaaa tttggtaaaa gaatatttat
     8641 acctggaaat ccatcttggt tgaaataatt ttatttaata aagaatctgt actagaattt
     8701 atcataggac aggttcttta ttaaatatat aaatttttaa atactttttc tcgtttataa
     8761 caattcagca atgtttttag tctataattg acattataaa atattaaact aaaaggagta
     8821 actgtatgga aaaaatatta gtagtagaag atgattctat attaaataaa actctatcat
     8881 ataacctaat tgaagatgga tatataatta cttcaaaatt tacagctaaa tctgcactga
     8941 agtctatttt tgaatgtgaa tttgatttaa taattttgga tattaattta ccagataaaa
     9001 gtgggtttga attatgtaat gaaataaagg gaaattacaa tataccaata atttttttaa
     9061 ctgctaatga tatggaatgt gatatgatta aaggctatga acttggagct ttggattata
     9121 taacaaaacc atttaatatc aatatattta aacagaaagt taaagctttt ttaaatcatc
     9181 ttacaataaa aacaaaacaa gattactata gggatggata tcttgaaatt aacttttctg
     9241 agttatctgc caatataaat ggaaatcaaa ttatatttac accattagaa tatcgtactt
     9301 taaaactgct tactgaaaat cctaaaagta ttttaacaag gaaagtactt ttagaaaaac
     9361 tctgggatat agatgctaac tttgtagatg aacatacttt aacatctgtt ataagtagga
     9421 ttagaagcaa aattgaaaaa gacaatctta agtatatcaa aacggtatat ggtatgggat
     9481 atatgtggtt aggtgaaaga aatgaatttt aaaaaatttt cttttagttc tatttattta
     9541 ataattgctg ttggtataat ttttacagct attttattta gtatctcaat ttatctaata
     9601 actgctgatt taaaactaat tgctctatta ttaatatata caatagtgct ttgtggattt
     9661 atagcattaa taatattttt acttcataaa aaaatagtta tattttgttc taaaatatgt
     9721 cacactttag atagtatgat gaatagtgaa gacaatattg aaattgaatt agaagaagaa
     9781 actattttat cacgtatcaa tcacagatta attagaatgt atgaagttat tcaagaaaat
     9841 cgcaatagta ttgctataga gaaagctgat ttacaagaac tagtttctga tatatctcat
     9901 caaattaaaa ctcctattgc aaatttaaaa atgattaata ctactctact aaatcaaaca
     9961 tttaaaacag aaattcaaaa ggatttttta atggatatgg aaaaccaatt agataaatta
    10021 gattttttaa tgcaatctat ggtaaaaacc tccagactag agacaggtat aatcacacta
    10081 tctaaaaaga aaaattcaat ttatgaaaca attgctacag cacttagtgg aattttgttt
    10141 gatgctgaaa aaaagaatat tgaagttaca gttgattgtg acccaaattt atatataatt
    10201 catgacaaga aatggacttc tgaagctatt tttaatatat tagataatgc tgtaaaatac
    10261 acaaattcta atggtaaaat acgtgtagct acagaatgtt gggttatgta tacaaaaata
    10321 gatattattg ataatggaaa aggaatatca gaaagtcatc aagcagaaat ctttaaaaga
    10381 ttttatcgtg aagaagatgt ccatgacata gaaggtattg gaataggtct ctatctttca
    10441 agaaaaatta tatctcttca aggtggctat attaaagtta catcagatat tggaaaaggg
    10501 tcgacttttt ctattttctt acctaataaa taaattttta aatatttcat ttgtaatgat
    10561 tctgcaacat tttggtttta tactgtattt acaagatgtt aaggaggtat tattatgagt
    10621 attttacaag taattaattt aaagaaatat tatggacaaa gttcaaacat tacaaaagct
    10681 cttgatggtg taactctatc tattgaaaaa ggcgaattta tttctattgt aggtacatct
    10741 ggtagtggca aatccacatt attaaatatg atgggaggat tagataatcc tacatctgga
    10801 aaggttatta taaagggtaa ggaattatct actatgactg atgaacagct tacaatcttt
    10861 agacgcagaa atataggttt tatatttcaa aattataatc ttgttccttt acttaatgta
    10921 tatgaaaata ttatcttacc aataggattg gatggagaga ccatagataa ggattttata
    10981 aatgaaatta taaatatgct ttctcttgaa gaaaaattat atgatatgcc aagtaatctt
    11041 tctggtggtc aacagcaacg tgttgctata gctagagcac ttgtaacaaa accagctatt
    11101 atactagctg atgaaccaac aggtaacctt gatagtagaa ctagttcaga tgtactgagt
    11161 ttacttaaag tgacaagtaa aaaattcaac caaacaattg taatgattac acacaataat
    11221 gaaattgcac aactaactga tagaattatt cgtattgaag atggaaaaat tgttaactag
    11281 gaggtgtagt atatgtttag aaataataat cgcacattta taagaaaaat agctttaaat
    11341 gatttaaaaa ttaataaact taaaacatat ctttcaggta tcattataat gatttctaca
    11401 tgtttattat taacagtgac cttagtttca tataatgcct ctgtagattt agttaatgca
    11461 tctccatatc atgctatata taaatctgta gatgaaaaag caaaaaatat actatataaa
    11521 aataaaaact ttagtggcgt aggaacttac aaattaattg gaagtaataa aaagacagat
    11581 tgtattatgt cgattgttta tgcagatgat acaacaatta aacttatgaa ttttcaacca
    11641 ttgaaaggca aactacctac aaataaaaat gaaattgcta tttcagaaaa atatttacag
    11701 gaatttggtt tagataaaga tattggagat agcattaaat taaattatta tgatgatatt
    11761 acgaataaag aagttcaatg tgattttatt attgttggat ttctagaaaa ttattatcaa
    11821 gataatgcca agcaatacta tacagtcgtt tcaaatgatt attttaagaa tatagcttct
    11881 acacctttac aaaataaaag taatactttt gacgagtcaa gacctgatac tgtagatgta
    11941 ttagttaaac tatcagaaga aaatacagag aaagattcag cttcaattaa gacacagtta
    12001 aagaaaattg gtttatcttt aggtattaag aaatataata tatacttaaa tgataactat
    12061 attgaatcaa atttaattga tggtgagcag attattacag ttttagtagt tggaatagtt
    12121 gttctttttt ctagtgtttt tgttatatat agtatttttt atatttcagt tgttaattta
    12181 gttcagatgt atgctaagtt gaaatcgtta ggtatgactt ctttccagtt aaaaaagatt
    12241 atttttctac aaggaaatat tttgtcaatt atttttattc cattaggggt tatagcttct
    12301 tgtataattg cttacataat tcaaccatta gcatggcaaa tgatagctga tttatttata
    12361 attttaattt tatcactggt aatgttttta actgttagaa tttctttatt taaaccagct
    12421 agaattattt ctaaaatatc tgctattgaa gccatgcaat atacagaaac taaatttaga
    12481 aaaaaagtaa aaaattttag ttatataaat ataaaaaatc tagcattaaa gaatatcgag
    12541 tcaaatagaa agaaaaattt gattgcatta atatcgctaa gtattagtgg agttttattt
    12601 atttcaatcg ctaatcttgc taattcaata gatttcaaaa aacaactatc tcaacaattt
    12661 gtctataacg aagattatat aattagcatt aagagtgata acttatatga gcatattaca
    12721 gaaatccaac aaaataatcc actaacagat tctcttaaga atgaaattaa atctattcat
    12781 ggagtaaaaa aattaattga atccaaatta attaaaagta atattataga accttacatc
    12841 aaagatagag acgaaaaaca atttattact ctaattaaag gtattacgcc tgaactttca
    12901 aaatcacttg aagaatatat tgtcagtgga aaaatagact atacacattt agattcagat
    12961 agtttaatta taaataaata tagaacaaaa tattatggat taaatttaaa agttggagat
    13021 acagttacat ttaatatctt tagtggaaat gatattatta aaaagaaaat gaaaattata
    13081 ggaatcattg acaattccaa tacagaaggt atgttttttg cttcagacaa aactttagaa
    13141 tctctaacac catataatac aaatttagat ttttctattt taatagataa aaagtattct
    13201 aaagaagttg aagaaaagtt gttaagtata attcagaaaa attcaaattt aatgctttat
    13261 tcatatgaag atgattatag aatgattact agagcatttc aatatataat tatagcttca
    13321 tacatttttg ttggtttaat ttcttgcttt ggtatactaa atatgaccaa taccctaata
    13381 aacagcgttt taattagaaa aaaagagttt gccttacttc aagccgttgg aatgacaaga
    13441 aagcaactgc gaaatatgct ttatagagaa ggtctaaaca taagtatcaa agctatatgt
    13501 acttcaagta tactaggtta ttttggttca aatctacttt gcacttttat aaaagatgta
    13561 attagattag actttattaa ttttaaattt tctatattta ctattttaat tttttcattc
    13621 gtattaattg gtatacaagt tttagtgact gaattacttg taagaaatat agaaaaaaaa
    13681 tcacttacag aacgcttacg ttctcagtaa gaatgtatat aaaaaatagc cattttttat
    13741 acaagtttta acataatttt ttaaaagcta aaaggataga aaattttgac tttacataat
    13801 tttctatccc tatacttgtt tttattttat acagatacac ttccttatat ctccgagcaa
    13861 catcaccaga gacataaaca agcatattcc ttattttcta aatatgtcaa ttgcatgact
    13921 tgtaacccct actaaatctt cacgttcaca agtagcaagg tgatatttta agtataatgc
    13981 aaatgcatca tcatccatag agtctactgc ttcacgcata tacaaagcca aaccatctga
    14041 tgcgacataa tgcagtctag ttacattaaa tgcagacatc aaatcatcaa tgttttcttt
    14101 acgtacaagc tcaaacaaat cctttggttc agacttagca gcaaatgtct gtggatcaat
    14161 caaacctttt tcaatatatt cagatacatt aatatttcct ctatggaacc cctcgtcaat
    14221 gagacagcca tcagatataa catatgcagc aaaaaccaca ccaccaggtt ttgttacacg
    14281 aattgcctca tgtaaagctt gttgtttatc ttctttgtta taaagatgat atagtggtcc
    14341 taacagtaac gtaatatcat atttattatc tgggaaaaca gataaatcca tagcattacc
    14401 ctgagtaatg cttatatttt catttgattg agtattttta tggaaaacct ctatattatg
    14461 ttcgactaat tccactgcgt caacatcata tccctgacgt gccagtgtgt gagaataacg
    14521 accagttgca gcgccaattt caagcacaca atcacctgat tttatgtact tatcaatgta
    14581 atgcatagta gtgagaaatt ccaccattcc atgttttaat gcaagtcgac tatcttcatc
    14641 ataagtatta taaaagtcaa ttaagtattg ttttgtttcc ataatttcac ctcttctata
    14701 ttagttaata aacatcaaat agacaaattc caatctggtt tttcttgtct tataaaagct
    14761 gttaatatat ttttcaacat tttttcagca ctatatctat ctaatgttag tttttttgtt
    14821 gctgttaaga cagctatgcc atgagtataa agaaataaat ctaaaaatat tacttttgca
    14881 cattccaact ctattccaat gctttccgaa aaaagtcttg cctttttttc attgtcaatt
    14941 tctttataaa aatcattcgc ctctttcatc atcaaatcta aatcatttat aaataacaac
    15001 ttaaataaat acgtttcttc ttgagcaaat tcaatgtatg aaagcggaag cagtaaataa
    15061 gggcttacac ttttagaatt acgataatta acaacatact gattataata ttcatatgca
    15121 aaagcaagaa attcattttt taattcattc atgttttcgt aacaagtaaa aattggtcga
    15181 gtggaacact gtaatttact tgcaatactc ctcgcattta cagcttcaaa tcccacttct
    15241 cttgtaatat ccaagactgt attcaaaatc atttcttttg ttatctttac tttaggtggc
    15301 atatatgttc acctctccat taaattttta taaaacacat atgttatata actatagtaa
    15361 catatcaaga aattatgtca agaaaaagct ttgcattttt ttgcaattcc taaaacttat
    15421 aaataaaaca tgagttgctg ttatctatta atagataaca gcaactcaga ttttccttta
    15481 aatactcttc tttttgtcta atatttcata tacacaatct tctattgaat taatatcttt
    15541 ttcttttctt tttttagaat caattaaaat gaaatacagt ataaaaccta cagcaagtcc
    15601 ccatgttccg cctttagcag caattacacc tcccataagt ccaatcaatc ccttctcaac
    15661 acctgtatta catagctcca ttgatagctt tgtacataaa tatccttgaa ctaaaagtgt
    15721 tactgacaat gctaatggta taagaggtgt agcaatctgc gcaattggat aaaagcagat
    15781 aaaaattgct gaaacccata ctaatgtacc cataccacta aaaacagact gcatggcttc
    15841 ctttccaatc ttgtaccttt gagcaacact tcctgtaagt gtcgcagcta atggaccaca
    15901 cattggtata tatggagaaa agattgccat aaaaatattt cttatcccac ttataacatt
    15961 agacctattt gaattgaaat ctatatattc atcttttcta ttttcacttt cagaaagtac
    16021 taaattttca cctgttataa aatcaccaaa tgcaattatg tatatcataa aagccattgg
    16081 tatcccttgt ataaataaca ttacacttgg aaaacctatt ccaaaaggag atactgtatt
    16141 tatcagattt ttaaaatcta ctatgtaaac taaattgtca aaactaaatc taggaatttc
    16201 tatttctcta ctaaatatac ctactattaa actcactaaa atagctgata ctacaccata
    16261 ttctcctaaa tgaaataaaa atttattctt aacttttaga gtatcaaatc tttcagaaaa
    16321 cattacaaaa caagcaacca aaattcctac tgttatagat ataggataag aatcaaatct
    16381 ccctttagga ttaaactctg atattatagt cgatataccg acacccatta gtattccacc
    16441 tttaatagaa tttggtatct tactaactaa ctttccagct acaccagtta tacccattaa
    16501 aataaatatt attcctaaat ctatttgtaa agctgttaat gcttgtattc tttcaatgcc
    16561 catgctgaac tttaacaaat atgcagttgt aagagttata gaagctgtaa tccagccagg
    16621 tacaactggg tctcccataa aaacatgcaa acagtataat attgagttaa tgatagccat
    16681 actccatgcc acttcatagg gtaatcccaa tatttgtgta ctaaggccaa ttgtgcctaa
    16741 acttgtcata cttgatatta taccttgaat agcttctggt ttctcaaatc tgtaatgtat
    16801 aaatggaatt cttatcttaa acattccaaa ttttatatga ggttgttccc ttctagcgag
    16861 tctttccata acttcactcc acctttcaat aactcttgta agtttacaac tttataattg
    16921 tttataaatt atcaatgtca ttaaaattag tatataacat gcaaattata taaacaataa
    16981 attaaatcta taaaattgat agattttgtg catatataga aagaccactt catttttagt
    17041 tacttaaatc acttaatatt agttatttaa atcacttgat aaaggttttt tataaaattc
    17101 aatagttcta ttccatgatt caaactgtcc ttttgaattt ccttctacag ttccacctga
    17161 agaaaaaatc cctccactca tactcataca taaagtctcc tttagaggca taatatatgg
    17221 aattggtaac ggatgaccca tattattgta tgttaataat tctacgttat atggataatt
    17281 attttcttca aagatttcta atgcctctaa acaagcttca taactattcc atatgttatc
    17341 ttcttttcca gcaatcataa gtatatctgc tttggaattt tctattttta ttctagcctc
    17401 ttttctattc tctgctcttt taagactttt cttataagta ctattaaatc caaatggaat
    17461 atcttttgat atattttttt tctgttcttc aaagaatata tcattgtcta ctttgatata
    17521 tggaaatggc tctcctttat atgaccatga tgaagtatca tttccactca tcaatccatt
    17581 aagagcttgg aaacaataaa tgtgaggttg tgatactaca acctttttaa tttgttcata
    17641 tcttgaagca agtaaaaagg ccaattctcc accctttgat gtcccatgta caaatatttc
    17701 tttagtactg gtaatttcat tttcactaat ccactcaaat actttttcaa aatattctaa
    17761 tggaacctct tctaatttat ctggtaaccc ttctaaagca aagtatgcaa ctgttagtac
    17821 attgaatcct ctagatgcaa gtggagctgc taaaaggtct agagcgtcta aatttccatc
    17881 agaaccacct agcattaaaa ttgttttatt attagaattt tgtttataaa ataattttcc
    17941 tataaactca tcagatatgt attttgtggt aatatcttct gatttaaata ttctctttaa
    18001 ttcaacttct tttctttcaa tagaagtttc aaatatcata ttcataatta ttggtttatc
    18061 aatagatata ttttctgcaa aatttttacc ttcagtttta gatttttcca gcgaataaat
    18121 taaccccata caattatcag ccttataagt accttctata ggttcaacta aatctaaatc
    18181 tacttctcct ttttcatttg aacaaaacac tccataagat gaaaattctt ctcccttgca
    18241 ccatggaaat tccattttca cacttatttt aagtttagta tttggaatta agtccgaaac
    18301 cataatctta agtctttcat ccacaaatga ttctacattg tatacattta ctttcataat
    18361 ctatttctcc ttataataat tatttatgtt ttatataaaa cagctttatc taattcaaca
    18421 taaataaatt tttaaattaa gacaccatat tgactatttc attttatcaa catattccat
    18481 ctttgtcaat atattgtctt ttttagtaat ttaaaattat gtagtctata tgaaatccag
    18541 ttgatttttg gctaattaaa gataggaatg ctattaggtt taaaatatat ctattcaaaa
    18601 taaaaaacca ctcattttaa gaatggtttt taatataaac tatttgttat taataaggat
    18661 atttttttat ttaaatttat ataatcgaac atctaatatt taatgtttaa taaaattatg
    18721 catttgcaaa agtatatatg gcattaactc ttgctacata taaattctca tcactacatc
    18781 caatatatct agcatatttt tcaatctttt cttttgcatt ttcaacatca ttttcatcgc
    18841 tctccattaa ctcatttata taaatatcca ttaatgacgc tttatattct cttatatcat
    18901 agcctaaaaa ttcagtcaat ctctcaattt ccttactaaa atctccatct tcttttacca
    18961 aaaattcgta tacatcttcc ctatatggat ttaaagttat tatttgtttt actactttgc
    19021 attcgtcagc cttcccaata aaaccatctt tataattatt aaataaggca ttagattttt
    19081 ttatagattc ttcatcaata taagaatgta ccgcagttat acgattttct ataagggcat
    19141 ctacaacagc ataatgaatt ttaaaacaat cattgtataa ataattgctc attttcttta
    19201 ttgtcgattc tatttctaag aagctcgcgt ggccattttt tttattttta tttggtataa
    19261 taaaagtttt attcaaatta ttaaaaagtc tcttaaaatc taaatatttt tcacaataaa
    19321 cacttttaaa ggtatcgata tctatagtta taattccaaa acttactata gtctcaattc
    19381 ctttcttaaa agactcttct atatattttt ctcctaaact taaacctcta tcagatattt
    19441 gctttagatt ttgaaaacta tttaaacaat ctctttcaaa gtttttagat atttctaaag
    19501 catataattt aaagattttc cttatcttat tataattatt ttttgtatca tcaaatctga
    19561 taatagctcc atttacatca aaagtaatca ttgagtttta acctccccat tagtttcatc
    19621 aataaaataa atttaatata aactttataa tcataatatt aaacttattt atactataat
    19681 tattaatttg atgataaaag ctattttcct tctaaataaa aaatttgtct aaagtttcta
    19741 aaaaatttag acaaatctta ggttattact ctttaaatta tttttaaatt attccaaata
    19801 cgccaaataa tattggtata aatatagctg gtaacaaatt ggctactttt atattcgtta
    19861 atttaagtac atttagacca agtcctataa ttaataaact tccagctgct gtcatttctg
    19921 tgactacagg atcacttaaa aatccattta aaaatgatgc tcccacacat ataatacctt
    19981 catacaaaaa tacagatatt gcagaaaaca ttacacctat acctaaagaa gcagtaaata
    20041 taattgaagc tattccatct ataacagatt ttacaaataa agtatcatga cttcctgtaa
    20101 gaccactttc taatgaacca actactgcca tggctcctat acagaataat aaactagatg
    20161 atataaaacc ctctgctata gaatcttttt tatttccttt agaaaattta ctttgtaaaa
    20221 aagcacccaa cttatttacc catttgtcaa tatctattaa ttctcctatc agagcaccta
    20281 cagctacgga tattattgtc actaatgtgt tttttccttc tagtgctcct gatataccaa
    20341 tgtacaatac acataaagca attccattca ttatagtatc gcttactctt tgaggtaatc
    20401 ctccttttat tataagacct actatacatc cacctgcaat tgctagacta tttgctatta
    20461 ctccaaacat tttttctccc ctcgattagc taattgaaat ttatcctatt attaaaacct
    20521 aaacatctat actttgtatt ggatatttta aatattccaa aaattcttca aaaccttttc
    20581 tagatatatt tacagtttta gtatttatgt taggatgaaa atttaagtaa tcttctttaa
    20641 ttacatcttt atctatgtaa agctcaactt ttctttctgt atcatttatt aatgaaaaag
    20701 gatttacact tccaggaaga agtttaagta cattgtatag tctttcaggt gaagcaaacg
    20761 ataatctaga acttcctatc ttatctttaa gtgagtttaa atcaacttgt ttttctcctt
    20821 taactacaat aagataatac ttatttcctt tggcatttct taaaaataaa tttttacatt
    20881 gacacccctt tgttattcca tctaaaatga ctaattgctc tgctgtatat acagcttcat
    20941 gctctactac atcatattca acatttaatt tatccaaaat atcatatatt ttagtttcat
    21001 tcatgctaga ttcattttca tcttgttctt ttttattatt taaatcactc atacttttct
    21061 ccttaaacac ttcatcataa tatcattgtg aggtattatt ctactatagc tattgctctt
    21121 actggagctc catcagcatc attatactta aatggtgctg ctataaatag aaatttttct
    21181 ggaacatttt ctaaatttgt aagattttct atgattattt ttcctttttc aaataaaata
    21241 tgatgcactt caaatactga tgtgtcatat ctatccactg atagcatatc tattcctatg
    21301 ccttttatat tagtattagc tatatagttt gcagcttctt ttgttaatgt aggataacct
    21361 acatagtact gctttttatc ccaaaatttt gaccaacctg atttaaatat tataaaatca
    21421 ctatttttta tttcatcttc atattttttt agatattcta attctatatt ctcatttccc
    21481 ttttcaagtt ccaatacata agctttccca acaaaatttt ctatatccaa cttatctagg
    21541 gtctcacctt tagtatacat atgtcttggt gaatccatat gagttccatt gtgagaaaat
    21601 acactaatta atgtttcttg ataaccattt tcttcaataa tagctacttt cttgatatct
    21661 ggtctattag gctctgcata tacaggcatg tcattatgtg tcacatgagt taaatcaaaa
    21721 accttcatct ttatataccc cctatgtttt tattttctat tatataccta aattcatttt
    21781 atttctatca gattttattc cattatatac catactacta tatatttttt ataaacctca
    21841 agggatttac cgaaaaatct tggaaagaat tatcagtaaa tataacatct tcacccaaaa
    21901 aacgtttata ccttccttct tctttcttta aagtaattct ctgtttttgt gaatgattat
    21961 taaaaaccat tatcttaaga ggaggttttt ttctattagt ctgcactctt tttaacatgt
    22021 attttatgtg catatcagct tcaggaaatg aatatccaca aaatattaaa aggtcagtat
    22081 ctcttaaagt tttttctgtt tcattccaaa cattacttaa aaatatgttt gacatattct
    22141 taaaatatgt tggtggcact ataataggtt ctgttaactc accacactct aaacattttg
    22201 tctctgaact attttcaata agcttcatga caccaccttc atgtggtgta agagttacac
    22261 tattacaaat ggggcaataa agccaattca atgacccatg tatcttatat aatttgatta
    22321 atggttcttt tggttttctc caatttaact cctctttaaa atttgtaaac tccacaccat
    22381 aatcaagcat aattggcaaa tcattcttat ctcttaatcc agtaactgta ttgtctatat
    22441 gtatatcata atttgcacta ataaaagtag tatcaagaag caaatcattt tccaaaagat
    22501 tttcaactaa aagtttatga tatttattat ctgttctagg tgtattatga attgcttcag
    22561 ccattagcag tattaaatat tgtcttaaaa aacgtatact atcacttctg ttatttagat
    22621 tttctagtcc aaaatttcta aatgcttctt ttctttgttc tgctaagtct aaaagaccta
    22681 aaacttcttc aaaggtagga aaatttgcat tatctatatt atcttttact atatcaatat
    22741 tgaacatctg tttaaagaaa atatacaatt ctctattcat tttggtatta taatttgtgg
    22801 gatttagttt tttaaaatat tcgctaaaaa gttcattttg aataggtaga ttctctgctg
    22861 ctgaagctcc tgctcctaaa aatattgcta ttttcatata gttcctccta atataaatat
    22921 tactcctatt tttatatcca ttaaatatcc atatcattta ttttatgata caacaataaa
    22981 gctgtctttt actagaaaaa atcctattac aagacagcct tatattatat taaatcttta
    23041 ataattcgta aatagaaaga gcatatattt gagtagcttt tataatatcc tctactatta
    23101 agtattcatt agcttggtgt tctgtttctg gttttcctgg aaaaatagct ccaaatgcaa
    23161 cacaattatc taaagctctt gcgtatgttg ctcctccaga tgaaagtggt gttccatcaa
    23221 gaccagtttc ttcttcatat acttttctaa gtgtcttaac aagaagagta tcttctggaa
    23281 catagattga atccaagaaa tcatattctt cataatttaa attgtattta tctgtcattt
    23341 tctttaactc ttttacaaaa tcatcctttt tgtatgttac tggaatcctt acatctattc
    23401 ctgcaaattc tttttcgtta tcaatagtta ctctacctat atttactgtt agtttacctg
    23461 atacatcatc cttgcaatta ggaattatat tgtttccatt agcatcttca ccaataactt
    23521 ctgctaaaaa cttaattata ttagaatcta tacctatatt attaagagct atacaaagtc
    23581 tagccactgc atttatacca gtatcacttg ctgctgaatg gacacttttt ccaattatac
    23641 agattttatt accttcaaca gtatattcaa aatttaattt atcaagttct ttttttaatt
    23701 tatcagagta ttttccaata tagattgctt tacctggaac tgcatttaaa gcctttccaa
    23761 ctgacaactc tatatcactt ttgctatcac aagtcaaatg aacttgtaaa agacctttct
    23821 ctgcatttgt tattgggaat ctagaatctg gagtaaatcc atagtttggt atttcttcat
    23881 tattttcttt atatttattt atacatctcc aaagattttc ttcatcagcc ccaaaaatga
    23941 atcttatcct cttattaaag tctacattta aatccataag agctttcact gcataaattg
    24001 ctgatattgt aggtccttta tcatcttgtg ttcctcttcc atacaattta ccatcttcta
    24061 aaacagcttc aaatggaggg taattccagc tttctaaatc accttctgga accacatcaa
    24121 cgtgaccaag tatacctatc attttttcac cttgtcctat ttctgcatat ccataatatc
    24181 catctttgta tactgtctta aatcctaaag atttacataa gtctaatata cctcttaaag
    24241 ctttatctac attttcacca aatgggcaat tttcagttgc ttcagatata acactaggta
    24301 tttttacact ttcttgtatt gaagaaatca tttcatcttg tagagaattt actttttctt
    24361 taatttgctc tttcatatta tcacccttca attttcatca taatatacta ataaatattt
    24421 tgacctttta aaaaaaaaat atgcttattt tttcagtttt tttataaatt caatcgcttt
    24481 taaagtcccc atttttgaat ggtcaaatgt aagtccacct tggaaataaa cattataagg
    24541 aggtcttatt ggagcatctg cacttaattc tatagaagaa ccttgtataa atgctcctgc
    24601 tgccattatg acttcacttt cgtatccagg catgtcccaa ggaacaggtt ttacataaga
    24661 atcgactggt gcagcttctt gtataccttc acaaaaactt attacttcat cagcattatt
    24721 aagtctaata cattgaataa tatcacttct taagtcatca tattttggta atacatcata
    24781 tcctaatttt tcaaaagctc ttgagcaaaa gatagctccc attacagcct gagatactat
    24841 ataaggcgcc aagaaaaaac cctgaagtac atttctagtt gttccaaaag taagaccaca
    24901 ttcttttcct attccaggag aagtcattct ataagatata agctcaatca aatcttttct
    24961 accagctata tatccacctg tcaatgcaag cccaccacca ggatttttta ttaaagagcc
    25021 agccataaca tctgctccaa catcagtagg ctctttggta tctaaaaact caccataaca
    25081 attatcaacc attactatag cttctggttt aacagacttt attacttcta tagcttcttt
    25141 tatatcacta attgaaagtg attttctcca agaatatcct tttgaccttt gtatcataac
    25201 gagtttagtt ctatcattta ttttgctttt aattccttct aaatctaagt ttccatcctc
    25261 taaaaaatct acatcatcat atgtaacacc atactctttc aatgaacctt tttcttctct
    25321 tatgcctata actccctcta aagtatcata tggtctacca gtaacagata aaatttcatc
    25381 tcctggtcta actatacctt gtatacataa agtaagtgca tgtgtaccat ttacaattat
    25441 tggtcttaca agagcatctt ctgtattaaa tactttagaa taaatttcct ctattttttc
    25501 acgaccaata tcattatatc catatcctgt agtccagtta aagtgcatat cacttagttt
    25561 agattcttgc atagctttta aaactttata ttgattatat tctcttattt cttttatttc
    25621 ttcaaactta tcttttattt cttccattat ttcttgtgat agtttaaaag tatcatcatc
    25681 tattccatag tagtctttta ataattcttt tgtctcatta agcattttat cacctgtctc
    25741 tttattattt tatttaaaca tgtaaaggag actattttac taaaacaaat aaattatagt
    25801 ttataagtta gtataagtct cctctcaatc atcttaaatt aaattaatta tctatctgtt
    25861 gttattatta ttgttgtttt ggttttgatt tgttagatta atatactttc caggttgtat
    25921 agttgataca gcatgtttat aaatcatgtt ttgttgtcta ttatcccctt ctattaatat
    25981 tatataacta tcaaacccct ttacaagccc tttaacttgc actccattaa ctaaatatat
    26041 agtaacaggt atcctttctt tccttgcatt atttaaaaac aaatcttgta aatttaaaac
    26101 tgtatttttc attagatttg taccctccca agattaaatt gaacttctaa aatattccta
    26161 ccttatattc tatataagtt at
//
LOCUS       DAEOSP010000036        26084 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.036, whole genome shotgun sequence.
ACCESSION   DAEOSP010000036 DAEOSP010000000
VERSION     DAEOSP010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26084
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.036"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            143..880
                     /gene="cas6"
                     /locus_tag="KRH54_001219"
     CDS             143..880
                     /gene="cas6"
                     /locus_tag="KRH54_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG8578888.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGKERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKVREFYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            885..2360
                     /locus_tag="KRH54_001220"
     CDS             885..2360
                     /locus_tag="KRH54_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578889.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATAKQKEDIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRFDIVKNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     VNTKNLGKKMIDNGNLSNLKELLLDNISFLKQSTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            2380..3393
                     /locus_tag="KRH54_001221"
     CDS             2380..3393
                     /locus_tag="KRH54_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HBG8578890.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYIGSTQLITNRGFLSSEFGRKYYDEKEENEVPR
                     DENFPTTQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVIGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            3409..4206
                     /gene="cas5"
                     /locus_tag="KRH54_001222"
     CDS             3409..4206
                     /gene="cas5"
                     /locus_tag="KRH54_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBG8578891.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIKKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            4233..6641
                     /locus_tag="KRH54_001223"
     CDS             4233..6641
                     /locus_tag="KRH54_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG8578892.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINNILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNKNVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDIDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            7082..8029
                     /gene="prmA"
                     /locus_tag="KRH54_001224"
     CDS             7082..8029
                     /gene="prmA"
                     /locus_tag="KRH54_001224"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBG8578893.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            8048..8806
                     /locus_tag="KRH54_001225"
     CDS             8048..8806
                     /locus_tag="KRH54_001225"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBG8578894.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            8808..10106
                     /gene="mtaB"
                     /locus_tag="KRH54_001226"
     CDS             8808..10106
                     /gene="mtaB"
                     /locus_tag="KRH54_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBG8578895.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            10149..10499
                     /locus_tag="KRH54_001227"
     CDS             10149..10499
                     /locus_tag="KRH54_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBG8578896.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            10662..10841
                     /locus_tag="KRH54_001228"
     CDS             10662..10841
                     /locus_tag="KRH54_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBG8578897.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            10871..11314
                     /locus_tag="KRH54_001229"
     CDS             10871..11314
                     /locus_tag="KRH54_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBG8578898.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            11425..11922
                     /locus_tag="KRH54_001230"
     CDS             11425..11922
                     /locus_tag="KRH54_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBG8578899.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            12115..12678
                     /gene="raiA"
                     /locus_tag="KRH54_001231"
     CDS             12115..12678
                     /gene="raiA"
                     /locus_tag="KRH54_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBG8578900.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            12891..13127
                     /locus_tag="KRH54_001232"
     CDS             12891..13127
                     /locus_tag="KRH54_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBG8578901.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            13140..14483
                     /gene="yqfD"
                     /locus_tag="KRH54_001233"
     CDS             13140..14483
                     /gene="yqfD"
                     /locus_tag="KRH54_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBG8578902.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKDFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            14422..15537
                     /locus_tag="KRH54_001234"
     CDS             14422..15537
                     /locus_tag="KRH54_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBG8578903.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            15594..16055
                     /gene="ybeY"
                     /locus_tag="KRH54_001235"
     CDS             15594..16055
                     /gene="ybeY"
                     /locus_tag="KRH54_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBG8578904.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            16061..16780
                     /locus_tag="KRH54_001236"
     CDS             16061..16780
                     /locus_tag="KRH54_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBG8578905.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            16899..17300
                     /locus_tag="KRH54_001237"
     CDS             16899..17300
                     /locus_tag="KRH54_001237"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBG8578906.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            17364..18257
                     /gene="era"
                     /locus_tag="KRH54_001238"
     CDS             17364..18257
                     /gene="era"
                     /locus_tag="KRH54_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBG8578907.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            18269..19648
                     /gene="mgtE"
                     /locus_tag="KRH54_001239"
     CDS             18269..19648
                     /gene="mgtE"
                     /locus_tag="KRH54_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBG8578908.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            19721..19876
                     /locus_tag="KRH54_001240"
     CDS             19721..19876
                     /locus_tag="KRH54_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBG8578909.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            19842..20615
                     /gene="recO"
                     /locus_tag="KRH54_001241"
     CDS             19842..20615
                     /gene="recO"
                     /locus_tag="KRH54_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBG8578910.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            20608..21207
                     /locus_tag="KRH54_001242"
     CDS             20608..21207
                     /locus_tag="KRH54_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBG8578911.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            21367..22245
                     /gene="glyQ"
                     /locus_tag="KRH54_001243"
     CDS             21367..22245
                     /gene="glyQ"
                     /locus_tag="KRH54_001243"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBG8578912.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            22245..24311
                     /locus_tag="KRH54_001244"
     CDS             22245..24311
                     /locus_tag="KRH54_001244"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBG8578913.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFDKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKIKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(24477..26024)
                     /locus_tag="KRH54_001245"
     CDS             complement(24477..26024)
                     /locus_tag="KRH54_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBG8578914.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DTFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
BASE COUNT        10681 a         2579 c         4684 g         8140 t
ORIGIN      
        1 gaataaaact aacagtaaag tatatttaaa aaattaaaaa acaaaattaa atttattatc
       61 aaaaaatcat tttatgatat gttttatttt tatattacaa atagagaata atgaattata
      121 ctagaaagta ggaggtgaaa atatgagcag aatttcaatt tttatggaat catgtaatgg
      181 caaatttcca aatgaaaatt caatgttgtc agtttccttt ataaaaaata ttttaaatgc
      241 agaaaacaaa gattttgcta aaaacatatt taactatggg aaagaaagaa aaaacaataa
      301 gcaaataaag gatataaata cagccattta cataccaaat cttataagaa gcaaaaatga
      361 gttacttgtt gatggagata taatattcaa tataagtttt tataattatt ctatgttcag
      421 taaattatac aatggaattt taaaagtaaa ggagctagaa tataaaggtt atagatttaa
      481 aataaaaaat ataaaaatac ataaagaaca cgagataaaa gaaaatggtg ttatatttaa
      541 aactatgtct cctataatag taaagaataa agaaggtaaa tatttagatg tagaagattc
      601 aaattatata gaatgtttaa actatatagc taatctaact ctggagagta taagaggtag
      661 tgggcttaga aagccattgg aatttatacc tcttaacttc aaaaaaagag tgttaaaaga
      721 aaagattcgt ggatttaaag tgagagaatt ttactatata aatgcttatg caggaacatt
      781 ttttttaaaa ggagatatgg aagatttaaa tgcactgtat aaaatgggaa ttggatatag
      841 aaggacggaa aatgcaggaa tggttgatat attaaagtag cgaggtgaga gaaataaaag
      901 ctaaatttta tagatcaggt gatattgtaa gagatattgg cattgtttac ttttatgaat
      961 tactaatgga tttaaaaaac gaactagaaa aaaatgatta taagctaaaa tttacttgtg
     1021 agttaaatag aaattattta tctttagaat ctgcagaata tatagaccct aagtatatat
     1081 ctgattatat attagaaaat caacttttta aggtatttaa aaatgggaaa aaggaaacct
     1141 tagaaaaaat atttagtaat atagataatt taacgtatgt aaattttatt gaagaattgg
     1201 ataaatcttc tgcaactgct aaacaaaagg aagatattaa aaaagatttt aaaaacagga
     1261 ggtttccata tgttagaaat agtggtaagt atggattaaa cacgagttta gaaaatatgg
     1321 aaacaaatgt aaaaagaata gtagatactg taattaaatc aaatatagag atagatgaaa
     1381 tagataaact tgatttgaca caatatgaag aaaaagatag tgtatgtaat gtttgcaata
     1441 taaataaaac aacaaaacta gatattgatt taagagaacg aaaggactct aagtataact
     1501 ttttatttag aggtgcagaa aaatcaggct ttaaaagaag tggacaagtt gaaagtaata
     1561 tttgttttga gtgtgaattt ttcaacttaa tgtgtctttt atatataaat ttaaaaagac
     1621 caatggtttt tgcatatacg aatgatttaa gagaattagc atttttaaat cataagataa
     1681 tgcttaaaag aaaaatgtat tcagataaat cattttataa aaaacttttg catgaaaaaa
     1741 taagttcaat tagactatat agatttgata ttgttaagaa taaaggtata attctaaagt
     1801 ttgactctat tatagagtat aaagaattat taaaaaaaat tgaacttata gatattatag
     1861 ataattataa tttctcaagg gagccagtaa acactaaaaa tttaggtaaa aaaatgattg
     1921 ataatggaaa tttatcaaat ttaaaagaat tattacttga taatataagt tttttaaagc
     1981 aatcaactgg aacttctcga gaaatggatt ttgtatctag ctcatataat atcggtttat
     2041 atataaaatt gtgttggatt gtagatggag gggaggagta taaaatgaaa aactatatgg
     2101 aaagtaattt aatatattca agagtaggta aagacttatt taataaaatg acagatgaat
     2161 ccagaagaaa aaatttttct atgaaagtga ttcaactact taagtctaat gatagaactc
     2221 agctatttca aactataatg catgtgttag tttctaatgg aatcttaata ggagaagggt
     2281 ttgtagaagg tctaatgcag agttctgaat tagaattaaa tacgaatgta ggtctattta
     2341 ttcaagaaat catgaaataa aaaaggggga aaatttaaaa tgaatgaaaa aagagtaaaa
     2401 ggagtaacat taactatact gacagaaagc ccagttccac tttcatatga ccaagggtat
     2461 ggaaattata caccaataaa aaaagaacaa tatagagaaa aaatacatgc aaaaactagt
     2521 atagccacaa taacatatga tttaagaaga atgctccacc aagaatatgg ttggaattta
     2581 agtaatattg tttttggaaa aaaaggaaat atatatccat ctattaagaa aaatgtagga
     2641 tgtgtagatg aaaatggatt agagactgat gtatttggtt atttgatacc acttaaagat
     2701 aaaggttcta taagtaaagc ttctccactt agaataatac cttttaggtc attaaatcca
     2761 tatataggtt caacacagct aataacaaat agaggttttt taagtagcga atttggtaga
     2821 aaatattatg atgagaagga agaaaatgaa gtacctagag atgaaaactt tccaacaaca
     2881 caagcattag caatagaaga gactttatca gattactacg tttatacaat aacattggag
     2941 cttgatagaa taggagtagt agaagtagaa gatgggaagt tacttttacc agaagaaaga
     3001 aaatttatga gtaaggaatt aagagaaaag gcagtaaaag atatattaga tgcaattact
     3061 gtgtttacaa gaaacataaa acattcatca gtactattaa aaccacttgc agtaataggt
     3121 ggagcttttg ataaagttgt accatttttt tgggatgatg tagattataa cgctgatagt
     3181 ggtgagataa atttagaggg agtcatagaa actatagaaa gttatagcct taaagaaagt
     3241 aacacaatac ttgctatcaa tgacagatta aaaatttcta ataaaaagga attaaataag
     3301 tttaatttaa gtaaataccc tgtaaaagag attaaaaatc tagcagatag attggaaatt
     3361 ggagaagata atatgtggta tttaaaagag taggaggttt tttgaattat gaaagtatta
     3421 caatgtaaac ttaagcaacc aactgctcac tatagagacc caaaagtatt tcaaaatgaa
     3481 tatataagta ctttaaattt accatctaag accacaataa tgggaatgat aacgtattta
     3541 tgtgatagaa gattaaactc tgatatagat ataggaataa ttggaacaca tcatcataga
     3601 gaactagaat tttcaagagg agagaacatt gatttttgga atgaatatac aaatatgaga
     3661 aaaggcaaag ataaagaaaa gtttttgctt caaggaaact actatgatta ttataaagaa
     3721 cataaagctc aaaatagcat attaaactat gaagttctta aagaagtaga actgactata
     3781 tttatttctt gcaaggatga tgaagaactt gaatttatta aaaaaaagct tgaaagtcca
     3841 tgtaaatatg caaatttagg aagaaaagaa gattttgtaa taccaagtga aaaagggtgt
     3901 tttgtgaaag aagttgagtt aaaagaggtt atacctatga atactcgtga tgctataaaa
     3961 gaaaatataa aattaaagaa tacatatgta agagttgact taagagataa agagaatgtc
     4021 gagactataa taaatcaagg tgtattgatt gcattacctc ataaatataa agatttagaa
     4081 gcaaatagag atgatagagt ttatgagttt tgtcattata tatatgttga taatgatggt
     4141 atatatccta aaaatattaa ggtaaatgta tgtattgata caaaagaagt ttttacatgg
     4201 ctttaaatta ttttgaaaga taggtgttta tcatgaaaaa tatcatgcaa aaagaaatat
     4261 tagctaagtc atacaattta agaactaatc aacaagagca aacattagtt gaacatatta
     4321 aggatttatt tgaagtatta gaatctatat tagaactaaa tctatattca gataaagatg
     4381 tagagatatt gaaaatatgt tgtgctttgc acgatttagg aaaaattaat tcaattatgc
     4441 aacaaaaaat ggagattaat aataaaatat cgtatagttg ctcagaagaa gagaggaaaa
     4501 agttagagtc tgacaaaaaa tctttaaaaa agatagcaag acataatata ttttctggag
     4561 catttttaaa agatatatta gaaaaaatga acttgtcaga agaagataag ttatatatat
     4621 ataaaagtat aatgttacat catggaaact atgaagatta tatgaggtta agtattagta
     4681 aagttcaaga agaaatttat gagtatattg aaaaaggcat tttagaaaaa gaagagttta
     4741 atctgaaaga tatagaaagt tatataaata acattttaaa tgtagacttt aaatttggtg
     4801 aagatgtatt ggattatgat tacatagata aattaagtga aagtatgttt atagacagtg
     4861 attataacaa tggtcaaata gatgattcag ttttaaatta tagaaagttt aagtatattt
     4921 tatataaagg gatgctgaac ttaatagacc atagtgcatc aagtagacag aaaggtataa
     4981 aattttataa cgattttact gatgaagaaa ttgataatat gattttaaat gagatatata
     5041 agagtcaggg taatttagaa aaaaataata tagagttcaa tactatacaa aaaaggttga
     5101 gagaactttc tggaagaaat gttctgactg ttgcatttac aggttcagga aaaacagctg
     5161 cagattatag aaagactttt aaaagaaaat tctttttagt gccaaataaa atatcagcag
     5221 aaagttttta tagaaaaaat atttttcaaa ataaaaatga tttagataca agaagtagta
     5281 atgattacat tggattactt catggagata taaacttata ttcagagaat gaagataatg
     5341 gagaacatga ttttgtattg acattaaggg atatagattt gagtataaac ttttgtaaac
     5401 cttgtgtaat tgcaacagtt gaccagttat tacttagtat gtttaaattt ccgggatatg
     5461 aaaaaatatt cgcagccgta aaaaatgcat ctataactgt tgatgaagta catttattgg
     5521 aaccaaagat gtttttgatt ttgatatatt ttatgcagtt tgcatgcaag tacttagatg
     5581 ttgattttca cttaatgaca gctacacttc caaaatcata taaagagcaa atgataaaca
     5641 agggaattat ttttcaagaa gaaagtaata aaaatgttac tgataaagga gaaatagttt
     5701 ttattgagag caacaaggaa gaagagattt gtgaaggtaa agatgtaaag gtttctttta
     5761 taaaggaaaa agaaatcaag tcaatagttg aagatgcttt agaaaataaa caaaaaattc
     5821 ttataataaa gaatactatt gattcagtca atagaacata tgagttttta aaagaaaatt
     5881 tatctgataa gtatggtggt gtagatatag atgttttaca tagtagattt aagttcaaag
     5941 ataaaaaaga gaaatatagt aaaatattaa atggagaggg tgatatttgg atatcaactc
     6001 aatcagtaga aatatccttg gatttggatt tcaatataat aataagtgat ttagcaacta
     6061 tggatagctt gatacaaaga atgggtagat gcaatagaaa taataaatat gaatatggaa
     6121 acttttatat tctacctagt gaagataaaa tttatgataa caaacttaag aacacaacta
     6181 aaaacatctt aaaggacata ttaaaaacta actctatttt tacaatggga attagaaaaa
     6241 ctattttgga tgattactat gataatagtg tagtcaaaaa atattttgaa gagaatttta
     6301 taagttgtga taaagaaatt aagaatattt atggtataaa taaagaaatt tttgatggat
     6361 tagatttaat ttttaatttt gaaccttaca aaaatattgt tgatagtaag aaggaagcag
     6421 ctaaaatttt tagagatatt gatgttagtt acaaaattat tctggaagaa gatttttata
     6481 aggaagatag ggagttacaa caagattcga ttcaagtatc agggtttata tttaatagat
     6541 tatattatta tggattaata agtaaagtag aaggatacat ggtactaaaa tctagtagta
     6601 agtttgagta taatagtact gtaggcttaa ttttgaagtg agcatatgtt taaatataat
     6661 atacctttag ccattgaaaa tacaaaaagt actactgtaa gtccactgta aaaactattt
     6721 tttaaaagga aaaataagtt gtgagataga gtgtttacaa gtgtatatag ataataaatt
     6781 tgaggtttaa gattaactaa gtgggtttca ggtacatcgc gaaaataatt ataagttgga
     6841 gattttgtat gtttaagatt aactaagtgg atttcaaata ttgatatagt agatataata
     6901 gaagttttat tattaggttt aaaattaact aatcggattt ttaaatattc tgactattat
     6961 ataaggatat tattagcttg aatttaaaac caataatata aaacaacaaa atattatatt
     7021 tatgatataa taaaagttat ggaaaaacag gaatattact atagagaaga gaggtaaact
     7081 tatgaataat tggatagaag taactataaa aacaacaaca gaagcagttg aacctataac
     7141 gaatatacta tatgaacaag gagctggagg agctgtaata gaagacccaa aagacttttt
     7201 attccaaaag aaaaatgaac tagactggga ttatgttgaa gaagaagtat ttaaaaagaa
     7261 tgaagaagat gatgttctta taaaaactta tgtttctgaa gaaaaaaatg tgatggaatt
     7321 tgtcgaaata ataaagcaaa aagttttagg attaaaagat tttggtatag atataggtga
     7381 aggaagtgta tcattggacc aagtaaatga agctgattgg gcaaatgcat ggaaagcata
     7441 ctacaaacca acaaaagtag gacaaagggt agtagtaaaa cctacatggg aagattatgc
     7501 tatgcaagag ggggatttaa tcatagagct tgacccagga atggcatttg gtacagggac
     7561 acatgagact actagtatgt gcattagaga attagaaaag tatgtgaata aagattctaa
     7621 agtatttgat attggctgtg gtagtggaat ccttgcaata gcagcagcta aattaggagc
     7681 aaaagaagtt gtagctgtag atttagatga agtggctgta aaagttgcaa aagaaaatgt
     7741 tcttgaaaat aaagttgaaa aaagtgtatc tgtaatgcat ggtaatttaa ctgatgtaat
     7801 aaaagataaa gcggatgtaa tagttgcaaa tataatagct gatataataa agatacttgc
     7861 aaaagatgtt caaaacttta tgaaagaaga tgctatattc atatcttcag gtataatttt
     7921 ggataaagta gaagaagtta aagaaagctt aattgaaaat ggatttgaaa tagttgaagt
     7981 tcaaaaatta ggtgaatggt cagcaatagt ttctaagctt aaaaaataag ggatatggtg
     8041 acgtataatg gatagatttt ttgttgaaaa aaataatata aatctacaag ataaaacttg
     8101 cactatagaa ggtgaagatg taaaacatat ttcaaaagtt ttaagatgca aattaggtga
     8161 gaaacttgaa atatgtgaca agaataataa tgagtatatt tgtgagataa tgaatataga
     8221 taagagtata gttaatttag aaattttaga aaaagttgat ataaatagag aatcagaact
     8281 taaagtaagg ctataccagg gacttccaaa agctccaaaa atggaaatga tacttcaaaa
     8341 attaactgaa gtgggtgtag aagaaataat attagttcaa actaagagaa gtgttgttaa
     8401 agttgatgac aaaaaagaag ataaaaaatt tgaacgttgg gaaagaataa tttatgaagc
     8461 agcaaagcaa agcaaaagag gtaagatacc aaaattaaga ggtgttttaa gttttaaaga
     8521 agctttagaa gatatgaaaa aaaataatgt aaatatttgc ccttatgaaa atgaaagaac
     8581 agtttcaata aaacatgcac taaaaaaatg tgattctaat atagatagtg ttggaatttt
     8641 tataggtcca gaaggtggat tttctgaaga agaaatagaa caaatacaaa aaaataattg
     8701 taatgttgta tcattggggc ctagaatatt aagaactgag acagcttctg ttgtagcatc
     8761 aacaatagct ttgtatgaac taagtgattt aggaggagaa aaataaattg aaaaaagtag
     8821 ctttttatac acttgggtgt aaagttaatc aatatgaaac agaagctatg ctagaactat
     8881 ttgagaaaga tggttatgaa caagtcaata gtgaagagta tgctgatgtt tatgttataa
     8941 atacatgtac agttactcat atgagtgata gaaaatctcg tcaatatata agacgagtta
     9001 aaaagaagaa tccagatgca ataatagccg ttgtaggatg ttattctcag gtatctccgg
     9061 aagaaatttt ggatatagaa gaagtaaatt tagtcatggg tacaaatgat agaagaaaga
     9121 ttgttgaaga aattaaaaag ataaattcaa gtaagaaagt tagtacagtt gacgacataa
     9181 tgaaagttaa agcttttgaa gaaatagaaa tcagccaaac taatggaaag actagagcat
     9241 ttatgaaaat acaagatgga tgtgacagat tttgtactta ttgtatcatt ccttatgccc
     9301 gaggacgcgt tagaagtaga gatattgata gtatagtcga tgaagtgaaa aaattggcta
     9361 ataatggcta taaagaggtt gtattaacag gaatacatgt tgcctcttat ggaaaagatt
     9421 taaaggatag ggatattaaa cttcttgatg taataaaaca aataaaccag atagaaaaaa
     9481 tagaaagaat taggcttagt tctgttgaac caattttatt tacagatgaa tttgtaaatg
     9541 aagttttaaa gatggataaa gtttgtcctc attaccattt atcacttcaa agtggatgtg
     9601 atgaaactct aaaaagaatg aatagacgat acacaacttt agagtacaaa actattgtag
     9661 atagattgag aagtaaaatg ccagatgtgg caataactac agatgtaata gtaggttttc
     9721 ctggagagac aaatgaagag tttaaaaaaa cgtacgagtt tttaaaagaa atagagcttt
     9781 cacaaatgca tatttttaaa tattcaccaa gaaaaggaac tccagctgca actatggaaa
     9841 atcaagttga ccctcaaatg aaacacttta gaagtgaaca acttttaaat ttaagtaaag
     9901 taaattttaa taaatttgca actaaattta tcggccgtga gttggatgtt ttgtttgaac
     9961 aaaatataga aggcaataaa tatgagggat taacttcaaa ttatataaga gttgttgttg
    10021 aaagtgacaa aaatatacaa ggtcagatat taaaagtgaa aataaatgat gtaaaagatg
    10081 aatatgtaga aggaattttg ctataacatg tagaaattat aatatttata agataggagg
    10141 tggaaagaat ggattgtatt ttttgtaaaa tagcgaacgg agaaatacct tcaactaaag
    10201 tttatgaaga tgatagagtc ttagctttta atgacttgaa cccagttgct ccatatcata
    10261 tattagttgt acctaaaaaa cattatgata gtttaattga tattcctgat aaagagatgg
    10321 acattgtgtc acatattcat gttgttatta ataaaatagc taaagagaaa ggttttgacc
    10381 aaactggatt cagagtcata aataattgtg gaagtgatgg agggcaagaa gttaaacatt
    10441 tgcactatca tattttagct ggtaaaaagc ttcctaatta cgaagcggga caaaactaaa
    10501 ctaaattatt gaaaaattga gttccttctt gataaaaata cttgataaaa gtaaaaattt
    10561 agtttataat aaacaaagtg ttaggttgta atgcatatta tgcaaaaaga acatagttac
    10621 agtcccagca taattgcgct attggaggga gggaaaaaga aatgtcagaa gtaagagtta
    10681 gagagaatga aacattagac agcgctttaa gaagattcaa gcgtcaatgt gcaatgtctg
    10741 gcatcatgtc agaagtgaga aaaagagagc actatgataa gccaagcgtt aagcgtaaga
    10801 aaaaagctga agcagcaaga agaaaaaacg ctaagaaata gtagtaaata agaataaaga
    10861 ggtgaagggg atgtccctta agcaaaagtt acaagaggat ttgaaatctt cgatgaaaaa
    10921 caaagataca gtgagaaaat ctgtagtaac tttaataaga gcttctataa agcagtatga
    10981 ggttgataat agggtcgagc ttgatgaaga tggaattatc gatgtaatag caaaacaatt
    11041 aaagcaacgt agagatgcat tagttgaatt tgaaaaagct ggaagagaag acttaataaa
    11101 agaaacagaa ggtgaaattg aagttttaaa agagtaccta cctcaacaat taagcgaaga
    11161 agagttagaa gaaattgtaa aatctactat atctgaagta ggggcaactt ctatgaagga
    11221 tatgggaaaa ataatgtcag ttatccaacc aaaagttaaa ggaagagctg acggaaaact
    11281 tataaataag ttggtaaaac aaaacttaca atagattgat atagcctaga gagaaatctc
    11341 taggcttttt atagtttttg ggaataacga gtcatatttg agcatatcta taatcaaaga
    11401 aaattttgaa agaaggggaa aaatttgatt atatcagtaa aagagattag gaattttttg
    11461 ataaacatat ttataccact aataataggt tacttaagta gcatcttaac aatgattatc
    11521 agtggaacag atatttcaac ctattattta caactcacaa agccaagctt tgcaccacca
    11581 gcttttatct ttcctatagt ttggactata ttgtacatat tgatgggtat atcttcatac
    11641 ctaatattaa gaaaaggtta caacttacct aaagtaagag atgcaatttt ttattatgga
    11701 ttacaacttg tattaaactt tatttggagt atactatttt ttggatttgg acttagattt
    11761 acagctttga tagatataat aataatgatt ttggtttcta taataatgat ttcgaagttt
    11821 tctaaaatag ataagagatc tggatacata aatttgattt atttaatgtg gcttgtttat
    11881 gcaggatttt tgaactattt tatatggttt attaataaat agaatgtaaa gattttgtaa
    11941 attaccctgt acttttaata atatcataac aaacgaaaaa taaatagact attaaaaata
    12001 atcattttca tatacaataa tattaattta aatacaataa atttataaaa attgtattta
    12061 aagattaaca tattaaagta ttagaattgg aaaggcttga agggagaggt ttttatgcaa
    12121 ataatagtat ctgggagaca aatgaaatta actgacggaa taaaaggata cgtagatgga
    12181 aaattaagta ggttagaaaa ataccttgat cctgaatcag aagtgaaagt aacagtaagt
    12241 gcgaaaaagg atagacaaaa agtggaggtt acaataattc ctattaacgg tcaaataata
    12301 agagcagaag atgtagaaga tgacttatat gcagcaattg atattgtttg tgacaaatta
    12361 agtagacaag ttgtcaaata taaaactaaa gttaaagaca aagttcaaaa taataagagc
    12421 ataagatttg aaaatcttga ctttatagat aatagcagtg aatttgatga ttatgattat
    12481 gatgatgaag aagatgaaaa tatagtaata gaaagaagaa agaaatttaa tgtaaaacct
    12541 atgagttctg aggaggcgat acttcaaatg gaactagtag gacacaattt ctatatgttt
    12601 agaaaccaag ataattttga aataaacata gtgtataagc gtaaagctgg tggatatggt
    12661 ttaatagaac aagattaata aaaattgtat acgtttagat tttatataag aaaaaataaa
    12721 aaggtaatca tgttgatttt ttatcaatat gattaccttt ttcttgttta aataatatat
    12781 tagatattag gtatttatta tataaaaatt ttaaaactaa tctgttacaa catcaataaa
    12841 gtgcataaat acctatttca agcataaaat taattaaggg ggtaatgctt atgttagaaa
    12901 tatccactga tttacaagta aatcaaccag ttattactgt tacaagtaat acgtttatta
    12961 gtatagaaaa ttatctatca atattagaat atgaagttga tttaataaga ataaagacta
    13021 aagttaaaac aattaaaata tctggggata aactttcact taagtatatt actgatagtg
    13081 agataggtat aaaagggatt atctacaatg ttgagtatgt tgattaggag gagatacact
    13141 tgataagttt tataagggga tactatgtta tagttgtgga aggagtagga ctcgaacaat
    13201 ttttaaatca tcttataaga aatgggataa gtgtttacaa tgtaactaga ataaaaaata
    13261 ctaaaatgga gtttcatata gatagacaag atataaaaga ctttaagaat gtttatagag
    13321 gaagtaagtt tgatataaag gtaaagcaaa aaactggggt cccttttatt atcaaaagag
    13381 tttataagca caaaggtatg tggatttgtg ctcttgtttc tttattttta ttgatgtcta
    13441 catcacagtt tgtgacagat gtatatatac aatctcctga aggtataaaa aaagaagctc
    13501 ttagaaatga gctttataag gttggagtaa gaccaggagt ctataagaag agtattgata
    13561 gaaaagaagt tagagaccat atgatgtcaa aatttaatga tgtggcatat ttatctatca
    13621 atgtaaaagg aacaaatata tttgttactg ttacaaaaaa ggcagaatcc ctaaaatcta
    13681 cagaccaatc aaattattgt aatgtaattg ctttaaaaaa tggtataata gaaaaggtaa
    13741 tccctagaag tggtaaatca gttattaaat ctggagacat agtccaaaag ggagatgtct
    13801 tattaaatgg agctaatacc aagtcaatac cagaagtatg ggcaagtact ttttatgagt
    13861 ccacaaaaaa ggcaagttat gtagatactg taaataaaaa aactggagaa aagaaaaata
    13921 tatatacact aagtttttat gataaagagt ttacaatgag aaaaaatata aaatataaag
    13981 attatgttgt tgaaaataaa gaaaaaaaac tatcaatagg aaactatact tttccaataa
    14041 agataaaaac gagtactttt tatgaaacaa aaaaggttga agtaaaaagg aataaagaag
    14101 agttaaaaaa agaattatct gaaaaagctt taaaagagtt agaatacata atcccagctt
    14161 cggctagaat aatagatgta aaacataact ttaaggttaa taaaaatatg ttagaatatt
    14221 taataacagt tcaaactagt gaaaatattg ctaaaattta tcctcttagt aagtcagaag
    14281 cagaaaggtt tataaaggaa gaaagtaaac cagatgaagg tgaagaggaa gttccttcca
    14341 atccagaaaa aagaccatta aatgatataa gaaatgagtt tgatgaggat aataaggata
    14401 ataaagatca aaacaattct gatgaaaata atagtaatca aaatagtaat aatagtcaaa
    14461 ataataatag caataacaac taataaaatt aaacttaaaa tcttaggagg actaattcaa
    14521 ttgataatac aaaagaaatt tacaatagca gatggtaact ttgaaagaga gttatttggc
    14581 aactttgatg aaaatgtaaa attaatagag aagactttaa atatagatgt aatacttaga
    14641 gaaggcaata taattttaat tggagaagaa aaaaatgtag attctgcatt aaaacttatg
    14701 aatgaattac accaaactgt atcaaatggt aagcatttag ataagcagag tatttcttat
    14761 tcattatctc ttttgcttga aggaagtgaa caaaaaataa aagaacttga ggggacaatc
    14821 gtcataactc aaaggggaaa agctgttcaa cctaaaactt taggtcaaaa agaatatata
    14881 aaacttatag aaaataatga tataactttt ggtgttggac ctgctggaac aggaaagact
    14941 tatcttgcgg ttgctatggc agtaaaagct tttaaaagag atgaagttag tagaattata
    15001 cttacaagac cagctgtgga agcaggagag agtctaggtt ttttaccagg agatttaaag
    15061 gacaaagttg acccatattt aagaccacta tatgatgcct tatttgaaat gttgggtgct
    15121 gataaattta ataagtattt agaaagaggt actattgagg ttgcaccact tgcatttatg
    15181 agaggtagaa cacttgataa ttcatttata atattggatg aagctcaaaa tactacttct
    15241 gaacaaatga aaatgttttt aactagactt ggatttggct ctaaagctgt agttacagga
    15301 gatgtaactc aaacagattt accacaaaat aagaagagtg gtctaataca agctacagag
    15361 attcttaaag gtgttccagg tattgggtct ataatgctaa cagatagaga tgttgtaaga
    15421 catgagttgg tacaaagaat aataagagct tatgagaagc acgacaaaag agaagaattt
    15481 aaaaaagaag aacgtaaaaa agcgaaaatg caggaaaaga ctttcaaaag aaagtaaaag
    15541 tgtagtaata tagatatata gcattattaa tgaataaaaa gtaggtgtag tgaatggatt
    15601 tgatactaga tgatagacaa gataaattag aagtaagtga ggagttaatt gaaaagataa
    15661 aggatataat catagaatgt ttagattatg aaggatatga tgataattat gaagtgagtc
    15721 tttcttttgt agataataaa gagattcatg aattaaatag agaatataga ggagttgata
    15781 gagtgactga tgttttatca tttcctcttt taagtgatga ttttgaggat gttgagttag
    15841 aagaagaatc cctaggtgac atagtagtat ctcttgaaag agccttagaa cagagtatag
    15901 agtataacca tagctttgaa agagaagtat gttttttaat atgtcatagt atgtttcatc
    15961 ttttaggtta tgaccacgat acagatgaaa atacaaaaga aatgagagaa aaagaagaac
    16021 acatattaaa taagttaaac ataacaaggg agtagttttt atgaaaccag aaaaaactcg
    16081 tcaaggtata attaaagcat ttaatgcagc tatagaaggg atattgtata catttaaatt
    16141 tgaaagaaat atgaaaatac attatttagg ttcagttgct gttcttataa taagtttgtt
    16201 ttttaacttt tcaaaattag aaatgataat gttacttatg tccatatgct tagttgtagt
    16261 tgctgaaatg tttaatacag ctatagaaaa agctgttgat ttagtaactg atgaatatca
    16321 tgttcttgct aagatagcaa aagatgttgc agctggaggc gttctagtgg cagcactaaa
    16381 ttcagtagta gttggatata ttttgtttta tgataaattg acagatatat ctggaatatt
    16441 aatttataaa ataagagaat cagaattaca tataaccttg atatgtatat tattggttct
    16501 tatagctgtt gtagttgtaa aagcattgac ttctacaggt actcctctaa aaggaggaat
    16561 gccaagtggt catgctgcct tagcatttgc tatagcaact gcaattacac ttatgactga
    16621 aagagttgtt gcctctactc ttgcatatat aatggctgta ttagttgccc aaagtagaat
    16681 tgaaggaaaa atacatacct tttgggagac tattgctgga gctttgttag gtgtgttgat
    16741 agctatatta gtatttcagc ttggaatgtt ctataattaa aataaatgta cataaaatat
    16801 ttaaaataaa acaaatctgt gctaattaaa tttttatgat ataatgtaga gtattaaata
    16861 aaaataactt ttaattattg tatacaggag gagttgttgt ggataacata gaattgttaa
    16921 ggttggcaga agatgccaga caacattctt atgctcctta ctcaggtttt agagtaggtg
    16981 cagctctttt aactaagagt ggtaaagttt atactggatg taatatagaa tgtgcatcat
    17041 taggtggaac aaattgtgca gaaagaacag ctatttttaa agcaatatca gagggtgata
    17101 aggatatcta taaaatagct atagcaagtg ataactcaga aaataatgag caaacatatc
    17161 catgtggaat atgcaggcag gtaattatag agtttggttc tgatataaaa ataataacgg
    17221 gatatactaa aggagaggtg tttgagcata gtataaagga tttgttgcca aactatttct
    17281 ctggaaatga tttaaaataa atttaaaagt ataaataaat ttaagtaatt aaaacaagaa
    17341 gataaaaagt taggagacag ttaatgttca aatcaggttt tgtcagtata gtgggtagac
    17401 caaatgtagg aaaatctact ttaatgaata atgtagttgg agaaaaaata gctataatga
    17461 gtgataagcc tcaaactaca agaaatacaa tacaagcagt atatacagat gaagaaatgc
    17521 agatagtatt cttagataca ccaggtattc acaaaccaaa aaataaatta ggtgaattta
    17581 tggtaaaggc agcaacagaa gcttttaaaa atgtagattt aatattattt gtagttgatg
    17641 attctaaaaa aataggtcct ggagatagaa agataataga ggatttaaga agtgttaaga
    17701 caccaataat tttagttgta aacaaaatag accaactaga ccaaaaagat gagctatttg
    17761 atataatcaa aatgtatgat agagaaggca tttttaaaga aatagtacca atatcagcat
    17821 taaaaggaaa gaatacggat accttaataa aagttataca aaattattta gaagaagggc
    17881 caaagtattt tccagattat atgattacag accagccaga aagagtatta atagcagaac
    17941 ttataagaga aaaagttctt cactatctta atgatgaaat acctcatggt gtagctgttg
    18001 aaattgaaaa aatgaaagcg agaaatgata aggaaatagt agatgtatct gctgtaatat
    18061 actgtgaaag agattcacat aagggaataa taataggaaa aaatggtagg aaactaaaag
    18121 gaataggtaa gtcagcaaga caagatatag aattgctttt aggttcacaa ataaatcttc
    18181 aattatgggt taaagtcaaa gaaaattgga gaaatttaca aaattatata aataattttg
    18241 gatataatga taaataaagg tgggttatat ggataggaaa ctggatgctt cccaagattt
    18301 attatatgaa gtaaaatcat taattgataa taataaagta ttggagttaa gagaattaat
    18361 agaggaatat catataattg atatattcga tataatggaa aatttagagg aagacatgaa
    18421 aattcaatta tttgaagtac ttcctttaga tatggcatct tctatattag aagaaggtag
    18481 tgtagagttt tttattagta ttttatctaa actggatgta gaacactcga aaaatatact
    18541 tgaacttatg tctcttggag atatggcaga caagttgagt gaacttgaag aagaagaaag
    18601 agaacacata ataaatcttt tgaatcaaga gaatgccgat tatgtaaaag agttactatt
    18661 ctatgatgaa gattctgctg gtggaacaat gaccactgga tatatttcta taaataaaga
    18721 tatgacagct cttgaggcta tagaccatat gagagaagaa gctgaagaag ctgaaactat
    18781 ttattatata tatgtggttg atgatgaaga aaaactagtt ggagtattgt cattaagaga
    18841 attaataata gctagagacg caaatatagt agaagatttg atgagtgaaa atataatctc
    18901 tgtttatgta gatgaagata gagaagaagc agtaagatta gtatctaaat acaatttaat
    18961 agcaattcca gtagtagaca gacaagaaaa attaaaagga ataataacag tagatgatat
    19021 aattgatgta atggaagaag aagcaactga agatatgtat aaatttgcag gaagttcaga
    19081 acatgaaaga gaagttgctg aaaaagaaaa ccctacattg agagagcaaa taatatcagc
    19141 acttagagga cgattacctt ggttaattat taccttagtt ggagggttgt tggcttcgtt
    19201 aatactttct aacttagact atataatgaa tcctgtatat gctccattag tattttttat
    19261 acctgtagta attggtatgg gtggaaatat tggaactcaa tcatcatctg ttacagtaat
    19321 aacattatct aataaggatt taaattttag caatgtggtt agagaaggta ttgtaggtat
    19381 tattacaggt cttttgtgta gtataataac aggtattgta atttactttg ttatgagaaa
    19441 gttagacatt gtattaattg tttcaatatc attatttata aatatggttt taggtgcaac
    19501 aataggtgcg tttatgcctg ttttattaaa aaaaatggat gcagacccat ctacagtttc
    19561 atcaccaatt atatctacag ctcttgatat aactggtata gcagtttact ttataataac
    19621 aacggcatta ttgtcaaaaa ttgtttaata actttatgta tgatttatcc tcttcttact
    19681 aaaaatataa gtataaatgt aagaaggggg ttttttaaaa atgaacaata tcagttggga
    19741 agtttttaaa aaaacaggta gtatagatgc atatttatat ttctgtgatt gcaaaaacat
    19801 acatgaagaa attgaagaag taagggagaa aaaagaagac gatgataatc ctgaacaccc
    19861 aggggatagt acttaaagct ataaggtata aggaaagtga tataatactt acactattta
    19921 caagaaagct tggaaaagta tctgcaatag caaaaggagc taaaaaaaat aagagctctt
    19981 tgctttcatc atcacagtta ttttcttata gcaattttac cttaaaaaaa caaggtaata
    20041 tgtacaaggt tactcaaagt gaaataataa aaagttttta taatatatcc tatgatatag
    20101 aggcattttc ttatgctaca tatattacaa aattagttga aaattctata cttgaaaatc
    20161 agactaataa taggctattt atattacttg cgcagactct gtatttgtat acacaggata
    20221 atacagacaa taggtttata acagcagcat tcgagttaaa atttttagac tatataggat
    20281 ttaaacctat agtaaataaa tgcattaatt gtgaatgtaa aattttacaa aattctgtgt
    20341 ttaatatcta cgaaggtggt atactatgta gtaagtgtag taaacttttt gataataata
    20401 taaaattaga cttaacaact ataagtctta tggaatatgt attaagaaat gatattttaa
    20461 catgtagtaa agctaaagta tcaaaatata taacacatga acttgagaat attctagaca
    20521 agtatttgaa agtttatgtt gataatatta attttaaatc attacacgta ttgcaaagtg
    20581 taaaaaataa taagggagtg gataatgatg agtagtaata taactattga aatggttgat
    20641 tcagtctttg aaagagttcc aggagcaact tatgcagaag caaaagaagc attagtaatg
    20701 tgcaatggag atgtaattga ggcagtaata tatttagaaa ataaaaaaaa tacttgtaat
    20761 tgtaaagcaa aatctgctaa agaaactatg gaagaagtat ttggcaaaga tggagaaaat
    20821 ataaagtttc aattaaaaga gcttcttaga aaaagtagtg tagtgagaat aatagttgaa
    20881 aaagatggaa aaactatgat gaatattcct ttaacagttg gagttgttgg attagctttt
    20941 gtaccattag ctactttagt tggattatca gctgctgtta taagtaaata tcgtataaaa
    21001 gttcaaaatg aagaagatgg agaaattgta gatttaggtg aattgaatga agaaaaactt
    21061 cacgttttga agaatatgat aactaatgca gcaaaagatg tgaaggatgt agtagttgac
    21121 aaaaaagaag atgacaaaga tgtaactgct gaccttatga aagaagaatc tgaaaagaat
    21181 aaagaagacg aagacgaaaa taaatagata taaagtgact tttaaaaaat gaagagaaga
    21241 taattataag aattatcact aacattgaca aattttatag aatttgatat tctaaaagtt
    21301 gtagaagaac tattaaaata gcctttcgtg tagacgagag gctttattgt tataggaggg
    21361 atttttatga attttcaaga gatgatactg gcgttgcaaa aatactggtc aaagcaaggt
    21421 tgtatcatga tgcagcctta tgatattgaa aaaggtgcag gaactatgaa tcctaatact
    21481 tttttaagat cattaggacc tgagccttgg caagtatgct atgtagagcc ttcaagaaga
    21541 ccagcagatg gaagatatgg tgaaaaccca aacagactat atcaacatca tcaattccaa
    21601 gttatactaa aaccttctcc agataatata caagaattat atttggaaag tttaaaagag
    21661 attggaattg acccaagtga gcatgacata agatttgttg aagataactg ggaagctgct
    21721 acagttggtg cttggggact tggatgggaa gtttggctag atggtatgga aataactcaa
    21781 tttacgtatt ttcaacaagt aggtaatatt gaatgtgagc ttgagacagg agaaattaca
    21841 tatggattag aaagattagc tatgtacatt caagaagttg atagtgttta tgatttaaaa
    21901 tggaatgata aaataactta tggtgaagta tttaataaag ctgagtatga aaactcaatg
    21961 tacgcatttg aattatgtga tgcagatatg ttgtttaatc tgtttgatat atatgaaaaa
    22021 gaagcattaa gacttatgga aaatggcctt gtaatacctt catatgacta tgtgttaaaa
    22081 tgctctcatg catttaatac attggatgca agaggtgcaa taggagttag tcaaagagct
    22141 tcatttatag gtagagttag aaatatggca aagactgttg cagaaacttt tgtaaaacaa
    22201 agagaagaaa tgggattccc tcttttaaag gacggtgaca aataatgaaa aattatcttt
    22261 tatttgaaat cggtgttgaa gaaatgccat caagatttgt aggcagtaca ctagaacaat
    22321 taaaaaacaa tcttagtaaa ttatttgatg aaaatagaat agcttttgat aaaataaatg
    22381 cgtatgggac accaagaaga cttactttag tagtcgaagg attgagtgat agacaaaatg
    22441 atttagaaga ggaaataaag ggtcctgcta agaaaattgc tgttgatgca gaaaataatt
    22501 taacaaaacc agcattagga tttataaaaa gcaaaggatt aagcgaatct gacatatatt
    22561 ttaaacaagt tggaaaagaa gaatatatat ttgcgactat aaagcaagat ggaaaagaaa
    22621 cttcagaagt actaaaagac atattaccag agactataaa atctgtagta ttcccaaaag
    22681 ctatgcgttg gggaggaaaa aatcttaaat ttgcaagacc tataagatgg atagttgcat
    22741 tgttaaatga taaagtatta gagtttgatt tagaaggaat aatatcttct aatgtaacaa
    22801 aaggtcatag atttttaggt gaaagtacaa tagaagttga ttctatagaa gattattttg
    22861 aaaaattaga aaaaaattat atagttttag accaaaacaa gagaaaagaa atgataagaa
    22921 aacaatgtat agaagtagca aattctttag gtggagaagt tgaatttgat gaagatttac
    22981 ttgaagaggt aacttattta gttgaatatc caactgcttt ttatggagaa tttgatgtgg
    23041 attatataaa actaccaaaa gaagttgtta taactcctat gaaacaacat caaagatatt
    23101 ttccagtact taaagaagga aaactgttac ctaactttat agctgttaga aatggtgatt
    23161 cttacagaat agacaatgtt aaagctggaa atgaaaaagt tttagaagca agacttgcag
    23221 atgctttatt tttctataga gaagatacta agagaaactt agaaagctac atagaaaaat
    23281 taaaaactgt tgtattccaa gtaaaactag gaactattta cgacaaaact ttaagacttg
    23341 aaaaattaag tgctgatata cttgatagtt taggtttagt agatgaaaag gcagatacta
    23401 taagggctgc taaattatct aaggcagatt tagttactgg aatggtagga gaatttgatg
    23461 aattacaagg ttttatggga aaagaatacg caaaagttgg aggagagaat gatgttgtaa
    23521 gtgaagctat ttttgaacat tatcttccaa gatttgcagg agatatactt cctaagacta
    23581 atgcaggagt tgccttatca atagctgata agttggattc aatagcaggt ttctttgcta
    23641 taggaataca accaacaggt tctcaagacc catatgcttt aagaagacaa gctttaggta
    23701 tacttagtat acttatggac agaaaagttg ctgtggatat aaaagtactt attgaacatg
    23761 ctttagacaa ttattctgaa ttagattttg ataaagaaga agttgttgag caaataatga
    23821 atttcttcaa tgagagagta aaaaatctat ttaaagattt aggaataaga tatgatgttg
    23881 ttgatgctgt tttaagttct gatataaatg atgtttctga tatgcatatg agagctttag
    23941 agcttaataa ttggctagaa aaagatgaat tagtggagat gcttactgca tttaatagag
    24001 tttctactct agctcaaaaa gcagaaagtg ataaaattga tgaatcttta ttaaaagaag
    24061 atgctgaaat taaactttac aatgaattta aacaagtaaa gataaaagtt aatgaattat
    24121 taaaggataa gaaatatggt attgctttag atgaatttgc atctttaaga ccagtagttg
    24181 ataatttatt tgatacagtt atggttatgg ataatgatga agctattaag aacaatagac
    24241 ttgctttact taaacaagta tatgatacta tgctagaaat ttgtgattta tctaaaattg
    24301 tttataaata aaatttaaac tatttataag aaataaggct gtctcaaaat agagatagtc
    24361 ttatttttca ttgttgagat gatgaaaata tatttgattt catagaaaaa gagtgtctca
    24421 taaactaaaa agttcatttt gagacaccca tgttttttgt aagtaaatat aataaactat
    24481 attttatact tatcagttat tttgtttaaa atattatcat ttgaatttac ccattcataa
    24541 aaattgcctg atgatgcgtc cgatatttta taaagcattt caggaatatc actagcttta
    24601 aaatctccaa gtgattttcc caatctagtc ttacctattt caaaactgcc tccaacaaat
    24661 aattcaaatg tatctgtaga tataccatca acattcttct ttttaccaca taaacctatg
    24721 ctaccaactt gatgaacacc acatgaattt aaacatcctg atatatataa cttaggtgct
    24781 ttatttaaaa tttcttcatt attttgtaat ctaaaataat ctatgatttc atgtaacatt
    24841 ttctgactat tttgtatgcc catttgacaa attggaacac ctatgcaaga tatactttgt
    24901 tctaattgag aattacaact aatagtttta cttatttcta atattctttt tgcttcattt
    24961 ccatttaaat ttaaaatata taatccctct gtcattccta gtctaatcat aggattttca
    25021 acattatcta attctttaag taaagtactt aaatcctttg ttaacaaaat acctccaact
    25081 ggatgtatat atacagtgta taaaccatcc tgtttttgag gtattagtct agaatcttgt
    25141 atatctattt taactccagg tttagagtaa tctatttctt caatattgag ttcaagacta
    25201 ccattttctt tttctaattt atagtattct ttaaattttt ctaagaatag ttcttcacca
    25261 agtgattcga ccatatatct aaccctagct ctgtttttat tttcataatt tccataatcc
    25321 ataaagaatt taatcatacc ttcaacacaa tataaggcat ctttcggctt tataagttca
    25381 tcaagttcta aagcaacttt gggttgttta cctagacctc cacctaagaa aactctgaaa
    25441 tatggttcat catttttcaa agtagcaaca aatcctaagt cttgtatact acaatgagca
    25501 gtatctgtgt aacaattaga ataagaaact tttaattttc taggtaaatg gtatgtagta
    25561 gcatttttga taaaatattt atcagtagca actgcatatg gagtaacatc aaagacttca
    25621 ctagggtcaa ctccagataa tggagataat ccgacatttc taggataatt accaccacca
    25681 cctctagtga atatatcatt ttttattcct tcttccatga tatcaacaat tgaatttata
    25741 gataaatcgt gcaattgtat agcttgtcta gttgttaagt gtatcttatc taagttgtgt
    25801 ttaatagcta tttcatagat tttattaagt tgatactgag ataaaactcc agcagatact
    25861 ctaagtctta tcataaaaga ttttttgtct ctttgagcat aaacaccata tccaccagaa
    25921 aatcccttat attgcatttt atttatttct ccatttaaaa attttgaaga atattcttta
    25981 aaattaggta gttcatttaa caatatcttt ttaaaattat ccattatttc acctcattgt
    26041 tagtcttgta taaaaatata tctattgtat aatagatata tata
//
LOCUS       DAEOSP010000037        25378 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.037, whole genome shotgun sequence.
ACCESSION   DAEOSP010000037 DAEOSP010000000
VERSION     DAEOSP010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25378
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.037"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..659)
                     /locus_tag="KRH54_001246"
     CDS             complement(<1..659)
                     /locus_tag="KRH54_001246"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG8578915.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     I"
     gene            complement(1022..1693)
                     /locus_tag="KRH54_001247"
     CDS             complement(1022..1693)
                     /locus_tag="KRH54_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG8578916.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            complement(1776..3992)
                     /locus_tag="KRH54_001248"
     CDS             complement(1776..3992)
                     /locus_tag="KRH54_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG8578917.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            complement(3995..4465)
                     /locus_tag="KRH54_001249"
     CDS             complement(3995..4465)
                     /locus_tag="KRH54_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBG8578918.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            complement(4527..5921)
                     /locus_tag="KRH54_001250"
     CDS             complement(4527..5921)
                     /locus_tag="KRH54_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8578919.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            complement(5994..7355)
                     /locus_tag="KRH54_001251"
     CDS             complement(5994..7355)
                     /locus_tag="KRH54_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG8578920.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            8089..9247
                     /locus_tag="KRH54_001252"
                     /pseudo
     CDS             8089..9247
                     /locus_tag="KRH54_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608406.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9399..9482
                     /locus_tag="KRH54_001253"
     CDS             9399..9482
                     /locus_tag="KRH54_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBG8578921.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            9607..10344
                     /gene="erm(B)"
                     /locus_tag="KRH54_001254"
     CDS             9607..10344
                     /gene="erm(B)"
                     /locus_tag="KRH54_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBG8578922.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            10289..10462
                     /locus_tag="KRH54_001255"
     CDS             10289..10462
                     /locus_tag="KRH54_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HBG8578923.1"
                     /translation="MSKYCLFLIVIYYLTGGNNSMSRFCKFGKLHVTKGNVDKLLGIL
                     LTASKELKRSGPH"
     gene            10706..10945
                     /locus_tag="KRH54_001256"
     CDS             10706..10945
                     /locus_tag="KRH54_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8578924.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSLIESGKSDPTL
                     KTLERIAELTNSTLVIDLIPNELEQVELQIEEEKQ"
     gene            11340..12830
                     /locus_tag="KRH54_001257"
     CDS             11340..12830
                     /locus_tag="KRH54_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBG8578925.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KKSMIGGRGVVLTSEEAIHENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTLIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKLVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREALGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALSEFFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            12833..13225
                     /locus_tag="KRH54_001258"
     CDS             12833..13225
                     /locus_tag="KRH54_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8578926.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            13226..14665
                     /locus_tag="KRH54_001259"
                     /note="aph(2'')-Ia"
     CDS             13226..14665
                     /locus_tag="KRH54_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HBG8578927.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(14795..15967)
                     /locus_tag="KRH54_001260"
     CDS             complement(14795..15967)
                     /locus_tag="KRH54_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HBG8578928.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFEPYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            complement(16002..18077)
                     /locus_tag="KRH54_001261"
                     /pseudo
     CDS             complement(16002..18077)
                     /locus_tag="KRH54_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610982.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
     gene            complement(18528..19163)
                     /locus_tag="KRH54_001262"
     CDS             complement(18528..19163)
                     /locus_tag="KRH54_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8578929.1"
                     /translation="MEGCAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRTEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYRTPYQVVKMINPNSKEDMELLREVLRNNHFDDEDE"
     gene            19675..20817
                     /locus_tag="KRH54_001263"
     CDS             19675..20817
                     /locus_tag="KRH54_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG8578930.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYVCDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYKNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTNLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            20810..21016
                     /locus_tag="KRH54_001264"
     CDS             20810..21016
                     /locus_tag="KRH54_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG8578931.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            21095..22288
                     /locus_tag="KRH54_001265"
     CDS             21095..22288
                     /locus_tag="KRH54_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG8578932.1"
                     /translation="MKEKRRDSKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKEKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNLQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDAIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSR
                     ETIQAFQRVMKKRPKAEPFVIDGQSNFLFVNHKGKPKVAIDYNALFVRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(22474..23820)
                     /locus_tag="KRH54_001266"
     CDS             complement(22474..23820)
                     /locus_tag="KRH54_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG8578933.1"
                     /translation="MSKYQKNVSMFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVV
                     GGAVGVSPTQITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVG
                     GNYGLASILGAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMA
                     GGVNSPTYASMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIIN
                     FDPIREATWFAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNIT
                     DKELSSGVIGSGICTMIGALFGGLPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGF
                     VPKFGALMTTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGI
                     TSVPAAQEAFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            complement(24148..25188)
                     /locus_tag="KRH54_001267"
     CDS             complement(24148..25188)
                     /locus_tag="KRH54_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8578934.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
BASE COUNT         8219 a         4575 c         3928 g         8656 t
ORIGIN      
        1 tctatagctt cttgagccat agttccatat ccttgcatta tccatgctgg tatttcttca
       61 tatccgtccc atgctgtatc ttgaaccatt actccatggt catcagcttc tgcataatca
      121 ttagctaatc ttactgcatc atcataattt aagtctgtta tactagcatc tgcaccttct
      181 cctttaattt tatttaatct aaattcagaa gaaccttttg gcattaatac aacagatttt
      241 tgacctaatt tattagctgt ccaagcaact cctctaccgt gatttccatc tgtagctgta
      301 aagaaagtta tttctcctaa tttttctttt atttctttcg aagtcaattt gtcataccct
      361 aactcagata tatctgtatc taatctttga gctaagtatc tacccataga ataagaacca
      421 cctaatactt taaatgcatt taatccaaat ctataagatt catcttttac atatactcct
      481 gcaacaccta atttttttgc taaaccttct aggtttgcta atggagtttc tttatattga
      541 ggaaagcttt tgtgaaaatt tctaacctta gttatttctt cttcatttaa aaatttgata
      601 caattttcct tatcgccctt tggcaaatta tttactttcc atttaatttc ttttaacatc
      661 taaaatacct ccaaattatt gttaaatttt tatactttaa tatataagca attaacatgc
      721 caatactaaa tatatactag atatttttcc atgactcttt agtatagaaa ttgcaatgct
      781 tttattattt tttcttttct atctttagtt catacaatgt atgtagaatt taataactaa
      841 aaaacctttt ctatcttctc actttttaat tctcattttt aaaatttaaa aataaatctc
      901 aaaacacttt gtttctatat tttttaatat aaaatttata aaatctaaat taaaacataa
      961 aatagtgatg caataatcta ttacatcact atttgtatat attcaataat ataataaatc
     1021 tttagtggtc tctaagaggt gatttttcat cttttttaac atctaattct tcttttacga
     1081 cattccattt ttcaagacca aacttagaaa ccataactgc ctctgctgca ccttttccaa
     1141 gtctttttac tgtgtccata cctgtctcat cctcttgggc accttttgct tttccatctt
     1201 tactccatat tgtaccacct atatagcatc cacctaaacc tccagacact aacatttcat
     1261 gcattaggaa gaaatttatt ataggaagtt ttgtcaattc ttggccacca tatctagttc
     1321 caccagtagt tatagctgca ccaaccttat tttgaagttt tcctggatga actaaataca
     1381 ttggacgaag tctattaaat actgctgtaa gttgagcagt tatattcata tcataaactg
     1441 gagaacctac tataattcca tctgcctcca atattttttg ctctaattct tggatatcat
     1501 cttttataat acacatagtt tcttttctta tacatgcatc acaatgtaca caaggactta
     1561 tttttttccc tctaactgac caatactcag tttctatacc aggtatagtt tctgcttctt
     1621 tcaaagcttc taacactgca tattctgttg ctcctttacg tggacttcca caaataccta
     1681 atatctttgc cataataata tctcctcacc aaataaatct atttttatta tctatttaca
     1741 aatacaataa attgttattt ctgctttaat ttctactatt ttaattttat tggtaatgaa
     1801 tgtatctcta catcagttgc attatatact gcatttgcaa cagctggcgc aactggtaca
     1861 acggcacatt ctccaatact cttaccacca aaaggcccag ctttttcata accatctaca
     1921 aatgcaactt gacattttgg catatctaca gttctaaata atttatatgt tttaaaatca
     1981 gtagctttag gtctaccttg ttcatcaaat gccaattctt cactcaatgc gtatcctaga
     2041 cccatatgaa tcccaccttc taattgtcct tctaaattta gaggatttat aactctacca
     2101 acatcatgta cagctacaaa atcaactact tttatatcct tagtttcttt atctactaaa
     2161 acttcagcaa aatgtacacc ataagaagta cgtccagctg gtgaagaata atcttcaaca
     2221 acagttaact ctcttaatga tttactttga ctatatactg cgacatcaga aagtgatgct
     2281 tttacatttt catcaagttt actaattaca catccattgt ctaggtataa atcttctttt
     2341 gatgtttcaa gtaatttttc tgcttcttct aatataagat ttttcaattt ctctgctgtc
     2401 tttttagctc ctccaccttc tacaaatacc cctctacttg catagtcacc aaggttgaat
     2461 gtacatgcat ccgtatcagt ttcaaatgta tcaactttat caggtgttat tcctaaaact
     2521 tcagctacca tcattgtttg catagttaca acaccattac ccatatcatg agctcctgtt
     2581 agtagtgtca acgtaccatc ttcatttaat ttaagagtca atgtaacaac atctctatgt
     2641 gcaccaaaaa ctccattacc atgtgcacct attgccatac ctatacctct taaatatttt
     2701 ccttcttctt ttggaagttt tttcttttca tcccacttaa acatcttctt accttgctct
     2761 atacaatcca atactctagg atttccaaga gaaccgtgga agattatatc gacatcatct
     2821 ggctctactg catttttatt ttgaaaatcg actaaatcca tacctatttc ctttgctatt
     2881 ttcgcaaatt gagcttgttg agccataaat atttgaggtg acccatagcc tctcatagct
     2941 cctgctattg gtgtatttgt atatacaggc atcccagtaa actttatatt tggtatttta
     3001 taaactttaa atactttatg actaagtgct cctataacat ttaaagcact tgatgcatat
     3061 gctcctgtat ttgtatatat ttttatgtct tgtgctatta cttcaccatc tttatttaca
     3121 ccagttttta attttacaaa agacccatga cgagttcttg ttgatatcat agattccttt
     3181 cttgtaagaa ccattttaac tggtcttctt gtcatttttg ataatgcggc tgctactggt
     3241 tcaattgtaa gttctaattt tccaccaaat gagcctccaa tagcaggtct tataactctt
     3301 actttattta atgacatccc aaaaatttga gatagtatta ttctaaatcc aaatgtattt
     3361 tggcaaggtg tatgtacagt taatttatca ttataatcat agtgtgcaat tacactatga
     3421 gtctctattg cactatgatg tattgctgga gtagagtatt catcttcaaa tatataatca
     3481 cattccttga agcctttttc tacatctccc ccatttatgt tgttttgaga aactatattt
     3541 gaatctccat gtatgcagat agcatcttct tttattgcat cttgtatagt aaatacagct
     3601 ggcaattctt catattcaac ttttataagt tttacagctt tctctgctat ttctggagtc
     3661 tctgcagcta ctgctgcaac acggtcacca acaaatctaa ctgtatctga aaatataact
     3721 tcattttcag gtattttgtg ctcataaaat cttaatgaac cattgtattt tctttgtgat
     3781 gtattgagat aagtagctat agctctaaca cctactactt tctcagcttc acttgtatct
     3841 atacttttta tttttgcatg tgctacaggg cttaaaagca tcttagcgta aagtacattt
     3901 tgtggtttca tatcagccac gtatttcatt tgaccagtaa cttttaaagc tgcatctttt
     3961 agagggtatg attttcctat taccttttcc ataattatct accccctaat ctagctgcag
     4021 ataattttac agcttctata atctttacat atcctgtgca tctacagaga ttttctttaa
     4081 aagcttctct aatttcatct tcattaggag atgcatttct atttaaaaga gcttgtgttg
     4141 ttataatcat accaggagta caatatccac attgtacagc tccgacttct ataaaagctt
     4201 cttgtattgg atgaagattt tctccattag caaaactttc tatagtgtat ataactttac
     4261 cttcgcagtt ttttgcaaga agtttacaag atttcatgtc aactccatct atcaaaactt
     4321 tacatgctcc acaactgcca ctattacaac cttgtttcgc tccagttaga tttaattttt
     4381 cacgaagtac atgcaaaaga gtttcattct tttcaacttc tatttcatac atgtaatcat
     4441 taatatttaa ttttattttt tccattttct cctcctacag ataaactaat gttgtaatag
     4501 aatattatta caatcttatt ttaatattag tataagctat tccacttatg tccttccatt
     4561 ctattagtta cattagctct cttacataat tcttctgctg catgttgtgc attaagcaac
     4621 acttgttctt cactttcaat tgtagttact tttccatttt caagtattac atttccatca
     4681 actaaaacac tctctacatt gtgcatacta gctgagtata ccaaagttga tacaggattg
     4741 tgaagaggta ttgattttgg agaaagcatt gagttaaata ttactaggtc agcttttttc
     4801 ccaatctcca aagaacctat ttcatcttcc attctaagag ttcttgctcc ctcaattgta
     4861 gccatttcaa gaactttctc tgcagaaatt gccagagggt cacaattatt tactttgtgt
     4921 tgtaaagctg ttaatttcat aagctctacc atatcttgtg cgttattact tgctgcaccg
     4981 tcaagaccta aactcacatt tatacctttt tttaacattt ctggcacagg tgcaacacca
     5041 gaagcaagat acatgttact tgctacatta tgagaaactt ttatgtcata ctttttaagc
     5101 atttccatgt cttcatcagt tatacataca caatgtactg ctacaacttc tggacctact
     5161 ataccccatt tttcaagtaa ttttatatct atttctccat gtatatcctt tgttgctgtt
     5221 cttgcaaatt ctgtttcaga tatatgaact gtaaaatatg aatcatattc tttaactata
     5281 ttccaaagca tcttacccat ttcttcagat atagcccaca ttgaagaagg agctacgcag
     5341 atttttattc ttccattttc tgaattatga tatttttcga atacccttct caaatctttt
     5401 tcaaccgttt ccacatcttc tataagctct ttgtgaatac ctaaatctat acaacctcta
     5461 cctacaaccc ctcttatacc taaatcttta tatgccttta ctattccatc tgtaagtcct
     5521 ggtctattgt gagtatgcat ataatcaacc attgtagtta taccactttt taaaccttcc
     5581 atacatccaa gcattgcagc atcataagta tcttgttctg ttaagaattt tgctgatgga
     5641 aacatcattg tatttaacca ctcatgaagt gccatatcat cacctaaacc ttttaataac
     5701 acttggaaca ggtgattatg agtatttata aagccaggga atattacttt tccattagca
     5761 tctataactt ttttagcatc aagatactta ggctcaatat caactgtatt tcctatgtcc
     5821 aagattctgt caccttgaat agctattgct ccatcaaata taacatctct gtctttatta
     5881 actgtaacaa ttattgcatt ttttattagt atatcaatca tcttaaatcc ccctttgaat
     5941 actttcatta caacatttta ccatatggtt ttctcatatt atatctaata catttatcca
     6001 atattttcct caagatttga agtaacgttt tcagcacttt tacgtgactt taatttaaat
     6061 aatgtttgaa gtaatatagc tattattgct ccagaaacta tacttgaccc tccaaggaca
     6121 gttccaattg tttctgggaa ttggtctaaa acttgtggag cagttgtaag acccatactt
     6181 gttgctatag atacacctgc tataagattg ttttcttcag tgaatccatc catactcata
     6241 atagagaaac cagacattgc tattgatgag aaaactacta aagttgctcc accaacaaca
     6301 caagatggta tagttgtcat tattgcaccc aatgctggag atatccctgc tagtaataat
     6361 acaagtgccc ctagtgctat tacaaatcta cttgtaactt tgttcataga tacaattact
     6421 gtattttggc taaatactcc agttggaaga gctgcaaata gtgatgaaat tatagaagtt
     6481 atagaatttc ctaatacaac tgatgctaat tcttcgtcag ttacctctct atccattgct
     6541 cctactgtag ctattgtaca ggcacccatc atatcagcta tacctattat gtaaataata
     6601 gtaaacatta ctattaactc tggtctaaat tctaatccaa aagctgctgg tgttggtaaa
     6661 gatactatag ctgctccttg aacggctgag aaatcaacca atcctaaaac taaagatata
     6721 gcataaccta ctaatattcc tattaatatt gaagaatttt ttactaaacc tttaccgtat
     6781 ttatttatca ttattattac aaaagcaact ataactccta ctgtgaagtt tattgggctt
     6841 ccaaatgttt gtgtgccttc tcctccagct aaattcttta tagctgttgc gaatagtcca
     6901 agtcccatac atgctattat agttccagaa actattggag tgaatatatg tctaattttc
     6961 ttaactccaa atcctacaaa gaatacgatt accccagcag caatctgtgc tccaaataga
     7021 gctgctaaac catctttagc tgctactgaa agacaagctc ctaaaaatac ataactcatt
     7081 cccatcatta caggtaatct tgaacctaac ttaaatcctt taaataaagg tattggatat
     7141 aactgtaaaa acgttgctaa acctgctgct aacatagaac cctgaatcaa cattgttgct
     7201 tgcccctggt taagtcctac cacgtttgct attaatatcg caggtaccat atttcctact
     7261 atcattgcta acagatgttg taaggccaaa ggtattgatt cctttagtgg tggtatccca
     7321 tcgacatcat attttgaagt attacgtgtt ttcataaaat aaatcctccc atacataaat
     7381 atcttgacta aatattttta taattatttt ctataaatat ttttatgacc atgaatttat
     7441 tgctcaaccg ataaacacag ctaaactgag ctatttttat aaaaaattaa caaatttcta
     7501 aatattgcct ccccgatttt caacttaaat gaacattaat tgcctggtat atatttattt
     7561 gttaaatttt ttattaaaat gaatagctat caaaaataat ctaacaatct catactaaat
     7621 aatttaaaca attatattta aattttatat ctgatttttg attatccgca taaacactat
     7681 actttcaagc attttgaaat ataggaaagc actcatttta ccacgaattt accacgattg
     7741 aatgagcctt gatatgacgt aagaatttga aaaggacacc gtatagtttg gtgtcctttt
     7801 acatgaaaat cattagtcgc tattgattgt tattatacat tttgttaatt gttcttattt
     7861 cttttacgtt ttttaccgaa aatcatgagt attgtccgag agtgattggt cttgcgtatg
     7921 gttaacccta aagttatgga aataagactt agaagcaaac ttaagagtgt gttgatagtg
     7981 cattatctta aaattttgta taatggtatc gtaatttaag acacttcaag agacatttct
     8041 taatgaatct gatatactgt aaacactaca gaaagaagga ttattattat gtctcgaaca
     8101 agaagaaatt tctctgccaa attcaaatca gaattagtga ttgaactgct caaaggagaa
     8161 aaagacttaa atacaatcgc aaccgaaaac aatattcagc cgaatcttct ccgcaactgg
     8221 aagaaggagt tcctcgataa agcatccgtg gtttttaatg acacacgaga ggataatctg
     8281 aaagaaaaac tcgctttaga acgcaaggaa aaagctgaat atgcgaaaaa agttggccag
     8341 ctcaccatgc aggtggattg gttgaaaaaa aaatctgaag aaacacttgg acctgactac
     8401 gagagtaaat ttagtccaaa accttttgaa gactaaagaa cttccagtta aaacaggagc
     8461 tacacttctt gatatcaatc gtaccagtgt ttattacaag ggcatgccca tatctcagga
     8521 ggagttggat tgcaaatcga tcatagatcg attacacacg gataatccgg cctggggagc
     8581 acgacaactg tctgctcaac tgaagaagcg tgggcatcag gttggtcgcc ggaaaacgcg
     8641 ccgttatatg aatgaaatgg ggattgatcc aatttatcca aaaatgaacc tttctaaacg
     8701 tatgcgacag gctaaagtct gcccgtatct gctacgtaac gccgttatcg accgtccaaa
     8761 tcaggcatgg tcaatcgaca ttacatacat ccccattaag cgtggatttc tgtatctgac
     8821 cgctgtgatt gactggtaca gccgctgtat cgttggctgg gaagtcgatg atactctgga
     8881 taccagaatg gtcataactg cgttaaaaaa ggcgtttata gtggcaaaac ctgttatcct
     8941 gaattcagat cagggctgtc agtttacaag caatgagtac atgaatttcc tcaaagagaa
     9001 ccagatccgt caaagcatgg atggtaaaag ccggtgggct gacaatatca tgattgaacg
     9061 atggttccga agcttcaagt acgaggaagc atatctgacc caatataaca atatcagaga
     9121 agcacggaag gcaatcggta aatatgtgca cacttacaac tttgaacgtt gtcattctgc
     9181 actcaataag caaacaccgg catcctgcta ttatccgatt ctgttactgg atgatcatgc
     9241 agcctaaggg gggattttct cccctaccca gttacatata tcagttcatt ataaaaagct
     9301 tagatttttg tcttgacaac tgaaccacta tattgtataa taggaattga agttaaatta
     9361 gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc caaatgcgta
     9421 atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac gcagataaat
     9481 aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa cagataacta
     9541 aaattacaaa caaatcgttt aacttctgta tttatttata gatgtaatca cttcaggagt
     9601 gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga aaaagtactc
     9661 aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga aattggaaca
     9721 ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac gtctattgaa
     9781 ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacat tcgtgtcact
     9841 ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta taaaattgtt
     9901 gggaatattc cttaccattt aagcacacaa attattaaaa aagtggtttt tgaaagccat
     9961 gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt ggatattcac
    10021 cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct taagctgcca
    10081 gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact tacccgccat
    10141 accacagatg ttccagataa atattggaag ctatatacgt actttgtttc aaaatgggtc
    10201 aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat gaaacacgcc
    10261 aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt taatagttat
    10321 ctattattta acgggaggaa ataattctat gagtcgcttt tgtaaatttg gaaagttaca
    10381 cgttactaaa gggaatgtag ataaattatt aggtatacta ctgacagctt ccaaggagct
    10441 aaagcgctcg ggaccccatt aagtattttt aaaagcatgg tcgcaagttt cactagcagc
    10501 catgctttta ttgaatcatt gtaggacatt ttaaagataa ttgcccactt taaaattaaa
    10561 catgtgctta gaatcgttct aggcaacacc aataacaatc actaatcaca agtgatttgt
    10621 gattaatcac ttgtgattta caaggtgtaa cgatataatt aaatggaaaa cgtgcaaaat
    10681 atttacataa acaggaggga gaaacttgga actagcattt agagaaagct taaaaaagat
    10741 gagaggtacc aaatcaaaag aaaaattctc ccaagaatta gaaatgagta gatcaaatta
    10801 ttcattgatt gaatcaggaa aatcagatcc gacattaaaa acattggaac gaattgcaga
    10861 attgacaaac tcaaccttag ttattgactt aattcctaat gaattagaac aagtagagtt
    10921 gcaaatagaa gaagagaagc aatgattgtc gttaatatat ttaacgaaat tgttgcttat
    10981 tttttttaaa agcggtatac tagatataac gaaacaacga actgaataaa gaatacaaaa
    11041 aaagagccac gaccaactta ttttccgcca agaaaataag ttgcgagcct taattgatta
    11101 ccaccaatca attaaagaag tcgagacccg aaatttggta aagtatttaa ttactttatt
    11161 aatcagatac ttaaatatct gtaaacccat tatatcgggt ttttgaggga atttcaagtc
    11221 tttaagaaga taccaggcaa tcaattaaga aaaacttagt tgattgcctt ttttgttgtg
    11281 attcaacttt gatcgtagct tctaactaat taattttcgt aagaaaggag aacagctgaa
    11341 tgaatatccc ttttgttgta gaaactgtgc ttcatgacgg cttgttaaag tacaaattta
    11401 aaaatagtaa aattcgctca atcactacca agccaggtaa aagcaaaggg gctatttttg
    11461 cgtatcgctc aaaaaaaagc atgattggcg gacgtggcgt tgttctgact tccgaagaag
    11521 cgattcacga aaatcaagat acatttacgc attggacacc aaacgtttat cgttatggta
    11581 cgtatgcaga cgaaaaccgt tcatacacga aaggacattc tgaaaacaat ttaagacaaa
    11641 tcaatacctt ctttattgat tttgatattc acacggcaaa agaaactatt tcagcaagcg
    11701 atattttaac aacagctatt gatttaggtt ttatgcctac gttaattatc aaatctgata
    11761 aaggttatca agcatatttt gttttagaaa cgccagttta tgtgacttca aaatcagaat
    11821 ttaaatctgt caaagcagcc aaaataatct cgcaaaatat ccgagaatat tttggaaagt
    11881 ctttgccagt tgatctaacg tgcaatcatt ttgggattgc tcgtatacca agaacggaca
    11941 atgtagaatt ttttgatccc aattaccgtt attctttcaa agaatggcaa gattggtctt
    12001 tcaaacaaac agataataag ggctttactc gttcaagtct aacggtttta agcggtacag
    12061 aaggcaaaaa acaagtagat gaaccctggt ttaatctctt attgcacgaa acgaaatttt
    12121 caggagaaaa gggtttaata gggcgtaata acgtcatgtt taccctctct ttagcctact
    12181 ttagttcagg ctattcaatc gaaacgtgcg aatataatat gtttgagttt aataatcgat
    12241 tagatcaacc cttagaagaa aaagaagtga ttaaacttgt tagaagtgcc tattcagaaa
    12301 actatcaagg ggctaatagg gaatacatta ccattctttg caaagcttgg gtatcaagtg
    12361 atttaaccag taaagattta tttgtccgtc aagggtggtt taaattcaag aaaaaaagaa
    12421 gtgaacgtca acgtgttcat ttgtcagaat ggaaagaaga tttaatggct tatattagcg
    12481 aaaaaagtga tgtatacaag ccttatttgg tgacgactaa aaaagagatt agagaagcgc
    12541 taggcattcc tgagcggaca ttagataaat tgctgaaggt actgaaggcg aatcaggaaa
    12601 ttttctttaa gattaaacca ggaagaaatg gtggcattca acttgctagt gttaaatcat
    12661 tgttgctatc gatcattaaa gtaaaaaaag aagaaaaaga aagctatata aaggcgctgt
    12721 cagagttttt tgacttagaa catacattca ttcaagagac tttaaacaag ctagcagaac
    12781 gcccaaaaac ggacacacaa ctcgatttgt ttagctatga tacaggctga aaatggaaga
    12841 agttttaatt gatgataata tggtttttga tattgataat ttaaaaggat ttcttaatga
    12901 taccagttca tttgggttta tagctaaaga aaataataaa attataggat ttgcatattg
    12961 ctatacactt ttaagacctg atggaaaaac aatgttttat ttacactcaa taggaatgtt
    13021 acctaactat caagacaaag gttatggttc aaaattatta tcttttatta aggaatattc
    13081 taaagagatt ggttgttctg aaatgttttt aataactgat aaaggtaatc ctagagcttg
    13141 ccatgtatat gaaaaattag gtggtaaaaa tgattataaa gatgaaatag tatatgtata
    13201 tgattatgaa aaaggtgata aataaatgaa tatagttgaa aatgaaatat gtataagaac
    13261 tttaatagat gatgattttc ctttgatgtt aaaatggtta actgatgaaa gagtattaga
    13321 attttatggt ggtagagata aaaaatatac attagaatca ttaaaaaaac attatacaga
    13381 gccttgggaa gatgaagttt ttagagtaat tattgaatat aacaatgttc ctattggata
    13441 tggacaaata tataaaatgt atgatgagtt atatactgat tatcattatc caaaaactga
    13501 tgagatagtc tatggtatgg atcaatttat aggagagcca aattattgga gtaaaggaat
    13561 tggtacaaga tatattaaat tgatttttga atttttgaaa aaagaaagaa atgctaatgc
    13621 agttatttta gaccctcata aaaataatcc aagagcaata agggcatacc aaaaatctgg
    13681 ttttagaatt attgaagatt tgccagaaca tgaattacac gagggcaaaa aagaagattg
    13741 ttatttaatg gaatatagat atgatgataa tgccacaaat gttaaggcaa tgaaatattt
    13801 aattgagcat tactttgata atttcaaagt agatagtatt gaaataatcg gtagtggtta
    13861 tgatagtgtg gcatatttag ttaataatga atacattttt aaaacaaaat ttagtactaa
    13921 taagaaaaaa ggttatgcaa aagaaaaagc aatatataat tttttaaata caaatttaga
    13981 aactaatgta aaaattccta atattgaata ttcgtatatt agtgatgaat tatctatact
    14041 aggttataaa gaaattaaag gaactttttt aacaccagaa atttattcta ctatgtcaga
    14101 agaagaacaa aatttgttaa aacgagatat tgccagtttt ttaagacaaa tgcacggttt
    14161 agattataca gatattagtg aatgtactat tgataataaa caaaatgtat tagaagagta
    14221 tatattgttg cgtgaaacta tttataatga tttaactgat atagaaaaag attatataga
    14281 aagttttatg gaaagactaa atgcaacaac agtttttgag ggtaaaaagt gtttatgcca
    14341 taatgatttt agttgtaatc atctattgtt agatggcaat aatagattaa ctggaataat
    14401 tgattttgga gattctggaa ttatagatga atattgtgat tttatatact tacttgaaga
    14461 tagtgaagaa gaaataggaa caaattttgg agaagatata ttaagaatgt atggaaatat
    14521 agatattgag aaagcaaaag aatatcaaga tatagttgaa gaatattatc ctattgaaac
    14581 tattgtttat ggaattaaaa atattaaaca ggaatttatc gaaaatggta gaaaagaaat
    14641 ttataaaagg acttataaag attgattata taatatatga aaagctatta taaaagacat
    14701 tagtattaaa tagtttaaaa aaatgaaaaa taataaagga agtgagtcaa gtccagactc
    14761 ctgtgtaaaa tgctatacaa tgtttttacc atttctactt atcaaaattg atgtattttc
    14821 ttgaagaata aatccattca tcatgtaggt ccataagaac ggctccaatt aagcgattgg
    14881 ctgatgtttg attggggaag atgcgaataa tcttttctct tctgcgtact tcttgattca
    14941 gtcgttcaat tagattggta ctctttagtc gattgtggga atttccttgt acggtatatt
    15001 gaaaggcgtc ttcgaatcca tcatccaatg atgcgcaagc ttttgaatat tttggttgat
    15061 cgatataatc atgaatcaat cgatttttag cctcacgcgc taagttaata tctgtgaact
    15121 taaaaattcc tttaacagct tctctgaaag attttgaatt ttttttagga atggtggtaa
    15181 agatatttct taggaagtga acttggcatc tttgccaact tacgttggtg aaggattttc
    15241 taatggcaga gactaatcct ttgtgcgcat cagaaataac gagttccgta ccttgtaaac
    15301 cgcgttcttt taggtattca aaaaatgttg tccaggtctc ttcgctttcg ccactttgaa
    15361 tcatgaagcc gataatttca cggtcgccat ctttggttat tccaatcgct atatgacagc
    15421 tttttgagag tactcgattt tcttctcgta cttttatata gagtacatcg gtcattaagt
    15481 aaggataatt tttttctgat aataaacgat tctgccactc gttaaccata ggttctagct
    15541 gttctgttaa gctagaaacg aaggacttag agacggattt accacaaagt tcttccacaa
    15601 tttttgatac tttacgagtt gaaacgcctg atacatacat ttccaacatt gaagccatga
    15661 gggctttttc gtttcgttga taaggttcaa acactgtggg tgaaaaatgg ccatcacgtg
    15721 ttctgggtac ttttaattct agcgtgccta cacgtgtcgt aaagctgcgc tcataatagc
    15781 catttcgttg actttgtcgg ttttctgttc gttcatattc ttttgcttga atatattctg
    15841 ttcgttgatt ttccattagt tgattaaata ccgttgttaa aatattttta gaaacgtcat
    15901 cctttacaga atattcaata atgctttgaa tctcttcgct tttcagtgta aaatgtactt
    15961 gggtcatgta aaagtcctcc tgggtatgtt tttgtcgtta ataaaacagc taatacaccg
    16021 gttgaaccag caaacataga agtaaacaat cctacgtttg tcatatcacc tgtttttaca
    16081 gggttctttt gtccgctagg ctttgttgga gtagttggct tgctcggttt ctgtggctgt
    16141 tgtggactta caggtttatt tgttactttt tcttttacgg taactgtaat tgttttttca
    16201 gtaattgcac catttttatc tactacacgg aaagttacat gataagtgcc agccttagaa
    16261 gtatctacat catttgcaat aatgttatct tttgttaaaa taatcgttcc gtcctcttta
    16321 tctgttgctg tcacatttgc caatgggtcg aacgtatcgc ctacattcaa tgtaacatca
    16381 tttgctgaaa tgattggagc agagttaagg tcgcctgtat tttgtttcac agttacagta
    16441 atcgtttttt tagcagaagc tccgttttta tctgttactt tataagtcac atggtaagta
    16501 ccagccttag aagtatctac atcatttgca acaatattat cttttgtcaa agtgatagtt
    16561 ccgtcctctt tatctgttgc tgttacattt gccaatgggt cgaaagtatc gcctacattc
    16621 aatgtaacat catttgcaga aatagttggt gctgtattat cttctccttg tgagtttaac
    16681 gataattcta cttttaaaac aacgtttccg tctttataag taccgcaatc atactcataa
    16741 gtaactggtt tttttacatc atctaccgtt ataattcctg tatctttatc taaagttcca
    16801 tttgcagtaa cttttacttt tgaaatatct attccttgtt caacatcttt ttttaagtcg
    16861 attttattcc ctttaatatc aatataaccg ttgcctacaa aagcatgatt attttcaaga
    16921 actttattct ctccgacatt taaccagtat aaatcatcgc aaattaattt tttaattttt
    16981 gtgtttttac ttatatcaag acctcttatc ccttgttcaa aaatttcacc gcaacgtagc
    17041 atttcaagtt ctaaattatt gcttaaatct aattttttaa tagttgtatc atgacaaaaa
    17101 aggcgtgtca gttttgtgtt ttttgttaca tctaattcag ctatatttgt catataacat
    17161 gatagttgtt tcaaattagg gttcttactt atatcaagtg tttttacttt tgtatctcct
    17221 atatctaaag tgactaattg cggattatgt gtgacatcaa gttccattaa tcctgtattg
    17281 ctacaagata gttgttctaa gttaggattg tttgttacat ctaaattgga aatagaggta
    17341 ctatctgcaa ataacgtatt caatttttca ttcttagata catcaagact gttaattgat
    17401 gtattatcgc aaacgaaagc ttccaaattc ggataacgtg ttaaatcaac gcttcttact
    17461 tttgaagaac gacaatctaa tctttttaaa gatgagtttt taaccggact taagtctgaa
    17521 acagaagtcg tagaaacatc taaagactgc aaatttgtta gatattgaat accctctaat
    17581 gaagatatcg ttttcatatc atgcaagttt aagaattggg cttttgatat ttcatcaaca
    17641 gataacacac tatcgccgtc tttatcaaat tctgcaatga tttgttctct aaatactttg
    17701 tctggaaaat gtgcttcatc aatagcaatg cttccagctt ttacttctgt atttttgctt
    17761 gcttcaacgg tcttatcttc ggtaaccact tctttttctg gtgttgcagg tgtttcctgt
    17821 tttgtacttt cttcttttaa agtttctgca ttttcagcag tttcttcctg ttccttgtct
    17881 gttatattct cttgtacttc tgcgttttct gttttctgtt ccacaggaac ttgctctgtc
    17941 ttgttttctt ctacaacggc ttcctgcaca acttctgtgt tttcctgtgc catgacagga
    18001 gtaggaagcc ctgtaagtgc agttgttgtt ccaatcatca atgcggaact tactgcaagg
    18061 acttgttttt tcttcatagt ttgttcactc cttatttttc ataaattctt tgcatagaaa
    18121 aacgcacaaa ctccccctta taactggata atctgtgcgt tctgtttatt attgatagaa
    18181 ttgaaaaaag ggcagaattt acttgtctgt ctgtctgtct gtctgtctgt ctgtctgtct
    18241 gtctgtcgca agcgtaacaa tgtgtgaaca tgactgtcaa ccctttcttt caaaaaaatt
    18301 caatccttgc ttctatacaa aaaacattat actggaaaca aacacctctt tcaatctttt
    18361 tatgcgtgca agataaaaaa attgagacat cacacttttc cttttttcct cttgagtacc
    18421 cacaacagaa aagaaaaagc cctgtcaaga gcttgccacc attggtggtg ctttgcactc
    18481 ttgatcgggc tttttgtttg ctgtatcttc tcgcaagagg aaaaagatta ctcgtcctca
    18541 tcatcaaaat ggttattcct taatacttcc cgtaacagtt ccatatcctc ttttgagttc
    18601 ggatttatca ttttcacgac ttgataaggt gtcctatatt tatgtccctc tatgctttcc
    18661 ccaaacatat ccatactgta taaatcatca tagcggtcta gtaatttttg aaacttcaaa
    18721 tgctggataa attctttgat tgggtaaagg tcggacgctt caatgctttt cccgttgata
    18781 tagtctgtaa tgtattcccg taacttttcc aactcatatt ccagccattc ttcctcgctg
    18841 tcaaagaagt tatcatagtg tccgtgtttc agttctgcat ttagacgttc ttctgttctt
    18901 aaaaactgac agacttcttt ttcagactga ttgacatact tccattctcc cgataataat
    18961 tcattcggcg ttacgtccag cacttccatt atcttttcca gcgtttcata agcaggatag
    19021 ttcaaccctc tttcaatctt ggaaaggctc tgcatattga taccgatttt gtccgcaagt
    19081 tcctgttgtt tcattcccct aaattttctt atggtctgta tattatttcc taaatggctt
    19141 attttcattg cacaaccctc catatctcgt tatatcttca ctgttataag tctatcatgc
    19201 ttaatatgat ttgtaaagac aaaaaaagat attgacaaat aagcatataa gaattactat
    19261 gctattgtga gtgaataagc acaatagcat gaaatacaaa aacattcaaa aagaaatacc
    19321 catctgatta aacccgtaga tatgaatatt ggttgaaatg gaacttgtta gaaaaggcaa
    19381 aacaggactt gctgacaaag tatttctgac acggtggcgg aagcctctca ggcttaccgc
    19441 catgacacct tgtgtcaagg ggaagaaatg ctttgtcggc tggcgagcct gcgagccttg
    19501 actgtgcccc cgttatctaa caggggggca ctatttcaca aaagacaagt caaaaaccat
    19561 acaaccccaa acctgtgagt agtaatgaga agttttaaga agatagtaat ttcacattct
    19621 tttgaatagt ggtttacagg tatttcaagg ctgttgtggg attggaggaa tacaatgaac
    19681 aatttagaat ttgcacttga aatgaagaac aaacgtcttg caagcggtct ttcacaagga
    19741 gaacttgcaa gtcttaccca tgtatcaaga tatagtatca accgctttga gaacggaaaa
    19801 gccaacgcaa gtaaagaaac acagaacatt atcctgcgtt gtctgaatta ctatgtctgt
    19861 gataagcctt tttatctgct cgttgattat ctgtctgtcc gttttccaac gactgacgca
    19921 ttagaagtta tccgaaaggt gcttggcatg aaagcggact attttatcca ttatgactat
    19981 gggtattatg gctataagga gcattatgcc tacggggaaa tcaaggtcat ggcttctgat
    20041 gatgaacaca tgggtgtatt tttagaatta aaaggggcag gctcacgcaa catggaatat
    20101 gtcttacagg cacagaacag ggattggtat tcatttttaa accgctgtct tgactgtggc
    20161 ggtgttatcc gacgttttga cttggcaatc aatgatatgt gcggtctgct ggatattccc
    20221 gttttgtctg aaaaatacaa aaatggcggt gcggattgtc gctgtaaaaa ctatgaaaat
    20281 gtacaaggtg gaaaactaag cggaaaaaac cgtaatttag caagtaccct ttatatcggc
    20341 tcaaaggcaa gcacaaaata tttctgcctg tatgaaaaac aaaaggaaca ggcaaccaaa
    20401 aagaaacata cggatattat caaccgtttt gaaattcgtc tgcgtgataa aaaggcagta
    20461 caggcagttg aggaattgtt attgacatat aaccctcatg ggttggtgtt ttaccttatt
    20521 actgattttg tgcaatttcc cgattatccg ctgtgggaga tattcatttc ccatgacagt
    20581 ttaccttttg aaatgaaccc cgtacctgtc aatatggaac gcactctgca atggctggaa
    20641 agacaggtca tgccgtcaat cgtgatgata gaggaaatcg acaggctgac aggctcaaac
    20701 tacatgaaaa tgattgatga atgtaccaac ctttccgaaa aacaggaaat gcttgtagaa
    20761 cagatgtgta cggatatagc agatgtaatc gaaagtgagg gggtgtttta tgagtaatgc
    20821 acaggatatt cccgtatggg aaaaatatac ccttaccatt gaagaagcgt caaagtattt
    20881 ccgtatcgga gaaaacaagt taagacgctt agcagaggaa aacaaggacg ctggctggct
    20941 cattatgaat ggcaaccgca tacagattaa acgccgacag tttgaacagg ttattgacaa
    21001 attggacgca atctaatgca aatgagcctt gtatgtgtta tgatgaacac aagtcatatc
    21061 aaggctcttt ccaacaagga aaggagcaga caccatgaaa gaaaaaagac gggatagtaa
    21121 aggacgtatc ctgcatactg gagagagcca acgaacagac gggaaatact tatataaata
    21181 tgtagacgca tttggaaaca caaaatatgt gtatgcttgg agattgacac ccacagaccc
    21241 gacaccaaag gaaaaacggg aaaaaccctc acttcgtgaa ctggaacagc agataagacg
    21301 ggatattgag gacggtatcg acagcacagg caagaaaatg acgctttgcc aactctatgc
    21361 caaacagaac ttacagaggg cgaatgtgaa gaaaagcaca caaaaacaac gggaacaact
    21421 catgcggtta ttgaaagagg acaagttggg tgctaggagc attgatgcga taaaaccctc
    21481 tgacgctaag gaatgggcgt tacgcatgaa agataaaggc ttttcctata acaccattaa
    21541 caaccataaa cgctcgttaa aagcgtcatt ctatatcgcc atacaagacg attgtgtaag
    21601 gaagaaccct tttgatttta agttaagtga agtcctagaa aatgatacca aagagaaagt
    21661 cgcattgaca gaggaacagg aacaagcctt actctcattt atcaagacag acaatgtgta
    21721 tcacaagcat tatgatgatg tgctgatact gttaaagact ggacttcgta tctcggaact
    21781 gtgcggactg acagtagctg atattgattt caagaatgaa gttgtaatta tcgaccacca
    21841 gttactaaag agcaaggaac agggctatta tattgaaacg cctaagacga aaagcggaat
    21901 aagacaagtg ccattaagta gagaaacaat acaggcattt caacgggtta tgaagaaacg
    21961 cccaaaggca gaaccatttg tgatagacgg acagagcaat ttcttatttg tcaatcataa
    22021 aggcaagccc aaagttgcga ttgattacaa cgccttattt gtccgtatgg taaagaaata
    22081 taacaagcac cacaaggaca atcccttgcc acatatcaca ccgcatacgc tacgccatac
    22141 attctgcaca agactggcaa gcaagaacat gaacccaaaa gatttacagt atatcatggg
    22201 acattcaaac attagtatca caatgaactg gtacgctcat gcgtccatag ataccgcaaa
    22261 atcagaggtt cagcgtctaa tcgcatagaa gtatttacca cgattttaac cacgcttgat
    22321 agcgaaaata taagaagata gacctagata tgtgaggttt accacaaaag caaaatgccc
    22381 gtagagccta taaaataagg ctttgcggac atttaaggag atataaaaag atagtcaaaa
    22441 agacatatat aatttctttt aaattattat atcttaatta gtctcttcca taacagctct
    22501 ttctttagcc tcatcctcta gacttttatt tggtattatt agatttaaaa cgaatgcaac
    22561 caatgttgca attactattg gagattctcc aaatactaac tttacaaatg aaggaaatgc
    22621 ctcttgggct gctgggactg atgttattcc catgcctaag gcaattgcca aaccaacaat
    22681 ggtaatattt cttgatgaca actcttctgt aataattaac tttatacctg tcattgttat
    22741 catactaaat atagatattg tagctcctcc taaaacagaa tatggtatag tagtcattaa
    22801 tgcaccaaat ttaggcacaa accccgacaa taacataaat ataccagcta ttccaagcac
    22861 aaatctactt attactttgt tcatagctac catccccacg ttttggctat atgatgatgg
    22921 taaacctcca aacaaagctc ctatcatagt acaaattcca cttcctatta ctccacttga
    22981 taattcctta tctgtgatat tcctatccat gcctcccatt gtagtagctg ataagtctcc
    23041 aattgcttga acagcattga ttatacttac aattactact gagattatta caggcatatc
    23101 aaattccatc ccaaattcaa gcggtttagg tatcgcaaac caagttgcct ctctaatagg
    23161 gtcaaaattt attataccca gaactaaaga tacacaatat ccaactataa ttcccaaaaa
    23221 tattgctgcc aattttgcat atccttttgc aaattgacta agacctataa ctaccactaa
    23281 tgttatagcc gctactgccc agttttgcat agaggcataa gtaggactat ttacaccacc
    23341 tgccatataa tttattgcta taggatacaa agataatcca attgtaaaaa ccactgttcc
    23401 tgcaactata ttcggaaaaa attttcttat cttttttatg aatatcccta ttaatactgt
    23461 agccactccc cctattaatt gtgcacccaa aatgcttgct aatccataat ttcctccgac
    23521 tgctacaagt gttggtacat aagtaaaacc aactccaaaa attataggca atcttgaacc
    23581 aatatttcct attggatata gttgtataag tgttgatata ccagctatta ttaatgcata
    23641 ttgcactaaa agtgttattt gtgtagggct aacacccaca gctcctccta cgactatagg
    23701 tactgtaaca gttccaacta tcattgctag tatatgttgc attgatagtg gcaaacactt
    23761 taaaattggt ggccttccat caatatcaaa catagaaaca ttcttttgat atttactcaa
    23821 ggttatcact cccaactatt tttaatgaca gttattatta tctcacacaa catcctttct
    23881 ttttttattt tgtcatctca acttaatatg aagcaattat aatgccaatt ttttaacata
    23941 attgttttac atatataatt tttattatat ttgttagtat ttgataattt attaaagcat
    24001 ttattttttt aaattctttt aaattttttt tatttttgga taataaaatg ctattacaca
    24061 aaaaaacact tagaaaatag ttttccaagt gttttttatc aattttataa tttcaaattt
    24121 tagaattgag aataaaaagc ctaattatta tacatctatt tttctgtaca atgtagctag
    24181 tgatattcca agttttttgg cagccagttt tttagactta gtatcatttc cgtaaaactt
    24241 taacactttc tttatatagg ctctttcaaa ctcttcaagt gtaagtatgt cttgttccat
    24301 acttatctca tagtcacttt cctcaagtaa ctttatattt atattattta acttaaaata
    24361 ttcaagtatc tttctattta ttattccttt atgtattatt ccatcttcct caagaaggtt
    24421 taatgccagt tctatagtat tttccaactc tctaatattt cctggccaag gatacttaag
    24481 caacatttgt tccacatctg gctctatatg gtacacgttt ttcttcatct ttctcgaata
    24541 tttgtctata aaattgttta ttaatatttg tatatcgtct cctctttctc taagtggagg
    24601 cacttctaaa ggtattacat tcaatctata gtataagtct tctctaaatt tattttcctt
    24661 aaccaactca aataaatctc tattagtagc tgatatgatt ctaacatcta tatcaattag
    24721 ttcatttgaa cctattctcg taaacttctt ttcctgtaat accctcaata actttgattg
    24781 tagttgaatt ggcatatctc ctatctcatc taaaaataca acccctccat tggccaattc
    24841 aaacttacca attttacctt ttgaatctgc tccactaaaa gctccttttg tatatccaaa
    24901 aagttcactt tctagtaaag gttctggtat agcagcacag tttatagcaa cgaatgtttt
    24961 attccctctg tttcctgcga gatgaatggc tcttgctatt aattcttttc cagtgccact
    25021 ttctccagtt atagctacag tagaatcact atttccaatt ttacaaattc tattcttaag
    25081 agcacgcatt acatcagact ctccaattat ggagtctact gttgttgttc cccaaatatt
    25141 atatacttcc attccaccat acattactga atcactgtgt ttgtccataa aatatcaact
    25201 ccaaataagt attctatata ataatcattc acattatcta tttaattata tcataacaga
    25261 aaagcttagt tataatttaa taatattatt ttaaaacaaa acttaataaa aagtatatct
    25321 acttagtttt aatttgatag atatactttc ttcattttac aaaaatgctc tattaaat
//
LOCUS       DAEOSP010000038        24226 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.038, whole genome shotgun sequence.
ACCESSION   DAEOSP010000038 DAEOSP010000000
VERSION     DAEOSP010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24226
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.038"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            109..300
                     /locus_tag="KRH54_001268"
     CDS             109..300
                     /locus_tag="KRH54_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBG8578935.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            455..1864
                     /locus_tag="KRH54_001269"
     CDS             455..1864
                     /locus_tag="KRH54_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG8578936.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTVEDFSSLETALNNIVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQIIDLEDI"
     gene            1995..2684
                     /locus_tag="KRH54_001270"
     CDS             1995..2684
                     /locus_tag="KRH54_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBG8578937.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2687..3622
                     /locus_tag="KRH54_001271"
     CDS             2687..3622
                     /locus_tag="KRH54_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBG8578938.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3619..4512
                     /locus_tag="KRH54_001272"
     CDS             3619..4512
                     /locus_tag="KRH54_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBG8578939.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4590..5336
                     /locus_tag="KRH54_001273"
     CDS             4590..5336
                     /locus_tag="KRH54_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBG8578940.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5337..6170
                     /gene="rsmI"
                     /locus_tag="KRH54_001274"
     CDS             5337..6170
                     /gene="rsmI"
                     /locus_tag="KRH54_001274"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBG8578941.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6193..6897
                     /locus_tag="KRH54_001275"
     CDS             6193..6897
                     /locus_tag="KRH54_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBG8578942.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7085..7513)
                     /locus_tag="KRH54_001276"
     CDS             complement(7085..7513)
                     /locus_tag="KRH54_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBG8578943.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7550..8980)
                     /locus_tag="KRH54_001277"
     CDS             complement(7550..8980)
                     /locus_tag="KRH54_001277"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBG8578944.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9112..9696
                     /locus_tag="KRH54_001278"
     CDS             9112..9696
                     /locus_tag="KRH54_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HBG8578945.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9712..10233
                     /locus_tag="KRH54_001279"
     CDS             9712..10233
                     /locus_tag="KRH54_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HBG8578946.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10299..10545
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10629..12566
                     /gene="metG"
                     /locus_tag="KRH54_001280"
     CDS             10629..12566
                     /gene="metG"
                     /locus_tag="KRH54_001280"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBG8578947.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTNFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12596..13366
                     /locus_tag="KRH54_001281"
     CDS             12596..13366
                     /locus_tag="KRH54_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBG8578948.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13703..14134
                     /gene="phnG"
                     /locus_tag="KRH54_001282"
     CDS             13703..14134
                     /gene="phnG"
                     /locus_tag="KRH54_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBG8578949.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14149..14724
                     /gene="phnH"
                     /locus_tag="KRH54_001283"
     CDS             14149..14724
                     /gene="phnH"
                     /locus_tag="KRH54_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBG8578950.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14727..15812
                     /locus_tag="KRH54_001284"
     CDS             14727..15812
                     /locus_tag="KRH54_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HBG8578951.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15832..16701
                     /locus_tag="KRH54_001285"
     CDS             15832..16701
                     /locus_tag="KRH54_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBG8578952.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16694..17560
                     /locus_tag="KRH54_001286"
     CDS             16694..17560
                     /locus_tag="KRH54_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8578953.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17601..18314
                     /locus_tag="KRH54_001287"
     CDS             17601..18314
                     /locus_tag="KRH54_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8578954.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            18315..19484
                     /locus_tag="KRH54_001288"
     CDS             18315..19484
                     /locus_tag="KRH54_001288"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBG8578955.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19488..20324
                     /locus_tag="KRH54_001289"
     CDS             19488..20324
                     /locus_tag="KRH54_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG8578956.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSNLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20325..21320
                     /locus_tag="KRH54_001290"
     CDS             20325..21320
                     /locus_tag="KRH54_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8578957.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21338..23026
                     /locus_tag="KRH54_001291"
     CDS             21338..23026
                     /locus_tag="KRH54_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG8578958.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     EFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23038..24105
                     /locus_tag="KRH54_001292"
     CDS             23038..24105
                     /locus_tag="KRH54_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG8578959.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
BASE COUNT         9677 a         2711 c         4326 g         7512 t
ORIGIN      
        1 ttttttgtca aaaaaaacaa aaaatacgta tttctttttt catttcggga aaactaaaca
       61 caggaattat ggactttata aaaatttaat tcttgaggag tgatataagt gaaaaaagtt
      121 gctttaattt tagctataat aggtgcgctt aactggggtg caattggtat tctaggtaca
      181 gacttaatag gtagtatctt tggtggaaca tatgaaatgg taagtagaat aatatacttc
      241 attgtaggtt tagctggatt atacttaata ccaagtttaa tgtctgatga tagagaataa
      301 tttatgcttt tatgttaaat acctcattct aaatgtttag aatgaggtat ttaattttat
      361 aaaaatatta atttgtttct tttttaatgg taaaatagat aagaattgaa gttttaaaaa
      421 gaagtattat gtaaaaaagg agtactatta atatatgttt attattaatg aattaaataa
      481 gattgtaaat gaagaagtaa tatcttttca tatgcctggt cataaaaagg gacaggttta
      541 taaaaagtta ggatataaag atatactaaa aaatttgtat aaaatggata caactgagat
      601 attaggaaca gataatttac actttccgga aggaattata aaaaattcac aaaaaaaagc
      661 atcaagagta tttaaaagtg attatacttg ctatttagta aatggtagta gctgtggaat
      721 acaatctgca ataatgtcag tatgtagtcc taaaagtaaa ataataacaa atagagactg
      781 tcatcagtct gttataaatg gatgtatttt aggagatata gatatagagt atgtgccttg
      841 tgagataagt gaagagacaa atgtcttaaa agggatatct ataagtaatg tgattgatat
      901 aatagataaa aacttggatg caaaagcagt actgcttact tatcctacat attatggaat
      961 gacatatgat ttagaataca tatgtaatta tgcacatagc aaaaagatgg tagtgatagt
     1021 agatgaagca catggtgcgc atttagggct tagcgagagg cttcctaaaa cagctcttga
     1081 gcaaggcgca gatatagttg tacagagtac acataaaact cttccagcat ttacacaatc
     1141 gtcaatgata catatacagg gagaccgagt tgatatagat aaaatattga acatgttaag
     1201 aattacagaa tcttcaagcc cttcatattt attattatct tctctggaat tagcagtaga
     1261 tatatatgaa aataaaggaa aagaattaat ggaagagctt ttaaataata ttataacttt
     1321 caaaaataaa gttgattcaa atattaatat atacgacaca aatgataaaa caaaaatatt
     1381 tatatcatct aaaaatatgg gtttaacagg ttatgaacta gaaaatattt taagaaacaa
     1441 atataacata caagtggagt tatctaatta ttatggagtt cttttaatat gtacaatagg
     1501 aaatactgtt gaggatttct ctagtttaga aacagcatta aacaatattg taaaggaaga
     1561 ctttaaattt gaaaagttag aaaataaaaa ataccctgtt gttattccaa agaaaatatt
     1621 aacacctaga gaagcatttt ataaaactaa aaaaagtgtt aaaatatatg acagcatagg
     1681 taaagtttgt ggtgaatgta tagttccata cccacctgga ataagtgtaa tatctcctgg
     1741 tgaagtaata agtaaagaaa taatagacta tctaatgttt tgtcattcta aaggcatgat
     1801 tgtgagtgga ttgaaagatg tagatttaaa ttttatacaa ataatagatt tagaagacat
     1861 ttaaaaatta tgatataaaa gtagatagaa gtagatagtt taaaattgtg aagagaaata
     1921 aatagactgc ttatatagag ctatctaata aaagtactaa aaaagtaaaa agtgtttgta
     1981 tggaggatat gaaaatgaaa ggtaagttaa taattataga gagtggttca gatgcaagtg
     2041 gaaaggctac tcagacagaa atattatatg aaagattaaa aaaagaaggc tatatgatta
     2101 aaaaggtaga atatccaaat tatgaatctc catcatctgc cttggtgaaa atgtacctaa
     2161 aaggagactt tggtaaaaat gcatcagatg tagacccata tatagcatca acttttttta
     2221 cagctgatag gtatgcatca tttaaaaccg attgggagga gttttacaat gaaggtggaa
     2281 taattatcgc agatagatat acaacctcta atatggtaca tcaggcctct aaaatggata
     2341 tttgtgatag agaagagtat ttaaattggt tagctgatta tgaatttaat ttatttaaga
     2401 taccacaacc tgattgtgtt atatttttag atgtgcctat tgaatttagt aaaaagctta
     2461 tggagaatag aaaaaataaa attacagggg aagaaaaaaa ggatattcat gaaagtgaca
     2521 tagagtattt agagaaatct tacaacaatg ctttatatat agcagataaa tatgattgga
     2581 aaaaaataaa ttgtgtttta gataacaaat taagaagtat tgaaagtata cataatgaaa
     2641 tttataaaat agttcttgat tcaataaaac ctatggagag ataaatatgt attttgaaaa
     2701 tattataggg caagattttg caaagaaata tttaacaaat tctataaaga aaaataaact
     2761 taataatgcg tatatgtttg aaggaatgga tggaatagga aaaaagaaat ttgcagatga
     2821 attatcaaaa ctattacttg attatgaaaa tctcgaaaat agtccagact atgtactcat
     2881 aaaaccagat ggaaatagta taaaaatagc tcaaatcaga aatttacaat cagatatagt
     2941 aataagacct cataaagact ataaaatata tataatcaat aatgctgaaa aaatgactgt
     3001 ggaagctcaa aatgcacttt taaagacgct agaggaacct ccaaactatg ctataataat
     3061 cttagtcaca aacaacaagg aatctttatt agaaactata aaatctagat gtgatataat
     3121 aaagttctca cctataccaa ttgaagactt aaaacgttat ttgataaata caggtataga
     3181 agaagaaaga gctcaattat tggctatttt ttcgagagga agtattgaga atgctctaaa
     3241 tctatctcag tcttctgagt tttcaatgat gagagaagat atacaacagt atattcagat
     3301 aatgttagat aaaaacatag tggagatttt aaatatacca aataatatgg aaaaatatag
     3361 aggtaagatt atagctttat tagatatgat gataaactat tttcgagata tcatcctatt
     3421 aaaagaaaat gtaaacaaga acatgttaat aaatgtagat aagctagttt tcattcaaaa
     3481 tatgagtgga aaaattagtt attctcaatt gtccaagatt attgatataa tagaagatgc
     3541 aaagagtaaa ataaaaagca attgcaattt caatataagc atacaagtta tgtctttaaa
     3601 tatatacgag gtgattaaat gataaagata gtaggtataa ggtttaaaag tgctgggaaa
     3661 atatattatt ttgaccctgt tgattttaat atagagcaag atatggatgt agttgtagaa
     3721 actgcaagag gcttggagta tgggaaagta gtcgttggtc caaaagatat ggatgaaagc
     3781 gaattagttt ctcctttgaa gccaattata agaatagcta cagaagaaga taaaaaagta
     3841 tatctagaaa ataaggaaaa ggcaaaagaa acttttgagt tgtgccaaca aaaaataaaa
     3901 gaacatgagt taacaatgtt tttaatagat tgtgagtata catttgatag gaataagttg
     3961 attttttact ttacagcaga aggaagaata gattttagag aattggtaaa agacttagct
     4021 gctatattta aaactagaat agaacttaga caaataggtg tcagagatga agctaaatct
     4081 ataggaggtc tagggccttg tggtagaaaa ctttgttgtt catcttggtt aggtgatttt
     4141 caaccagttt caataaaaat ggcaaaagac caaagtctat ctctaaatcc aacaaaaata
     4201 tctggaatat gtgggagatt attctgttgc ttaaaatatg agcatgatgt atatagtgaa
     4261 gcaatagaaa aaatgcctgt tgtaggtgca ttggtgcaat cagatgatgg aaaaggaaaa
     4321 gttgtagaag tgaatccttt attagaacaa attaaagtgg aatttcaaga caagacagta
     4381 aagatataca caagagaaga tataaaaata cttagagagc ctaaaaaatg tgatggctgt
     4441 ggtggtaaat gtaaggataa tgatggatta gatgaagcca cactaagaga gctaaagaaa
     4501 ttagaagatt aaatttaagg tagggctttt cctacctttt ttaaagcaat taatgcttaa
     4561 agttattaat atttgaggag aatttatata tgcaattaaa agattcagaa agaatagatg
     4621 atttacaatt aaaaggtctt aaaattatac aagatactaa tggtttttgt tttggtatag
     4681 atgcagtttt attagccaat tttactaaaa taaaaaaaga tgccaaagta gtagatttgg
     4741 gtactggaac tggaattata cctattttga tagctggaaa aagtgaagct aaaaaaatta
     4801 ttggcgtgga aatacaagaa gatgtatatg aaatggcaac tcgttctata aaattaaatg
     4861 gattagaaga aagagttgag attataaatg aagatataaa atacatagac aaagtcttag
     4921 acataaatgg ttaccatgta gtcacatcaa atcctccata tatgcatatt gatggaataa
     4981 aaaatccaaa tgataaaaaa gcaatatcaa gacatgaagt aaggtgcaat ttggaagatg
     5041 taattagagc tgcatccaga ttggttatgc ctagaggaaa attcttcatg atacatagac
     5101 caacaagatt ggttgatata ataactttag ggagaaaata taaattagaa ccaaaggtta
     5161 ttcaatttgt gcatccaaga cctcaaaaag caccgaactt agttttggtt caatttgtaa
     5221 aagatggaag gccagagctt aaaatattgg acccattgta tgtttatgga gaagatggga
     5281 attatacaaa agaattaaaa gcaatatata ataataaaga tataggagag ttgtaaatga
     5341 gcgggaaatt gtacatatgt ccaacaccaa taggaaacct agaagatata acatatagaa
     5401 ctttaagaac attaaaagaa gtagacttga ttgcagcaga agatacaaga catagtataa
     5461 aacttttaaa tcattttgaa atctcaaaac cactaactag ttatcatgaa cataataagg
     5521 attcaaaggg agattatttg ataaataaac taattgatgg tgaaaatata gctctgataa
     5581 gtgatgcagg tatgcctggt atatctgacc caggagaaga gataataaaa caagctatac
     5641 aaaataacat agagatagaa gtattgccag gtgcaacagc ctttgtaacg gctttagttg
     5701 gctctggtat ggacacacat agatttgttt ttgagggctt tttagataga gataaaaaag
     5761 ttaggaaagc acaattggaa gaattaaagg aagaaagtag aactataatt ttctatgaat
     5821 caccacatag attgaaggat acactaaaag atatgcttaa aattcttggt aataggaata
     5881 tttctataaa tagagaactt acaaaaaaat atcaagaaat aataagagaa aatatagaaa
     5941 ctaccataaa aatatttgag gaaaaagaag taagaggaga atttgtatta atagtagagg
     6001 gttttcatgg tgagaagtca aagaaagaaa attatgatgg gcttacagat agggaatatg
     6061 ttttaaaact aattgaaagt ggtatgaaca aaaaagatgc aataaaaata gtttgtaaag
     6121 atagaaagtt aaagaaagat gtggtttata aacaagtttt agatttataa aacctgaatc
     6181 tgaggtgatt ttatggaaat agggaaaatt ataatagaga gtttaaagag ggaagataca
     6241 cataaaatat ttatagaatt agataataag catgaattag aaaaaataat tcctaaaata
     6301 agctgtatga agtctgtagg tgaatgtaaa tatcatgtag taaattgttt tgaacattct
     6361 ataaacgcat taaaagaatt agaaatagta ttaaatgata aggatttttt tccaatacat
     6421 ctaagaaaac atgtgtcaaa ctatttaaac acatatattg aagaaggaat aaataagtta
     6481 catgtattaa agctgggaac atttttacat gatataggta agcctgacag tatgacctta
     6541 gatgaaaccg gaagagttca tttcacaaat catgaaaaaa taggtgctca aataattgac
     6601 aatatgggaa taaagttaga gttatcaaca gagacatata gacttataag taaatatgta
     6661 agatatcata tgatactttt gtctctatat aagaaaaatg atttgagtag gaaagaatta
     6721 attaatgtat ttaacttagt agatgaggat actataggtg taataatgct tgggtatgca
     6781 gatatagtgt ctacagtaaa attattgggc aagagtggag aagtcagtgt gttaaagaca
     6841 tatatggaat atattttaac aaattacttg tataagagta attatacaca tgtataaatt
     6901 tagttaatat aattgaattt tagatgatta attgaagttt gaaatatacg gatttcaaaa
     6961 acataaaaga agttgtcttt gttgtaaaat tatgaaagac aacttttttt atatcaaaaa
     7021 ttgaaatttt attaaggata cttaatggtc tattaattaa taaaccatta gtatttaatt
     7081 tatattacaa tattgatttt gcttgtattg gcgttgatag tataacagag gtcttagtag
     7141 aaccaatttt ctttatagta tcaatcacat tttctagctc gtacatatct tttacaatta
     7201 cttttaaaag taagcaatca tcacctgtta tatggtgaca ttctacaatc ctagggtcct
     7261 ttgcagctga ctcaataaat tctgtatatc cattgcttgg aagagaaata tgaataaatg
     7321 cctttataac tctgcctaat gaatctgggt tgacaatagc tttatatcct tctataacac
     7381 cagactcttc taatcttttg actctttctg aaactgcagg agaagttaaa ccaactattt
     7441 ttcctaagtc cttcatagaa attctcccat catcctgtaa aatttctatg attctgtaat
     7501 ctgtaacatc cataaataaa cagtccccct taaataaaaa taattaatgc tataaaactt
     7561 ttttagaatc tatcatttgt gtttttaaat accaaagatt tctagataaa tcaaccttat
     7621 aaatgtgtgg ttttgaaagt cttttgtatt gttcccagat agtacttaat tcatattgta
     7681 catgattttt tatttcaaga acagcttttt tattatattt gcatctgcct tttatataca
     7741 atggtttaag aagctcttta actttgtaat ttgtaaatac cttcgttttc catgtataag
     7801 taggatggaa gatttctaaa ggtttagact catctataac ttcatcatgt aacataatca
     7861 aatctgcctc tgccttattg tcttcgttat atattcttat aactttcttg taaccaggat
     7921 tatttatttt ttctggattc tcagaaatct ttatcttagg ttcaagaact cctttttcac
     7981 aagatgcagc taatttataa actcctccaa gagaaggtga atcgaaagat gtaatgagct
     8041 ttgtacctac tccccatgaa tttatagtag ctccttctgc tttaagtgat gttatggtat
     8101 attcgtctag gtcattagat gcagtgatgc tgatatctga aaagccagca ttatctaatt
     8161 gttttttagc ttctttagat aaataagcta aatctccaga gtcaagtctt atacccatag
     8221 gtttgtatcc tttttcagaa atatttttga atacttttat tgcattagga actccactgt
     8281 ttaatacatc ataagtatct acaagtagta ggcatttatc tggataaaca tcagcatatg
     8341 cttggaatgc ttcaagttca ttatcaaatt tttgtaccca actgtgagct tgtgtaccaa
     8401 ttataggtat atcaaacatt ttccctgcta gtacgtttgc agttccagca caaccaccaa
     8461 ctacagcagc cctagcccca tatatccctg catctggacc ttgtgcacga cgtaatccaa
     8521 attcaaatac agggtctcct tgagctgcaa agcaaactct agaggcttta gtagcaataa
     8581 gagactgaaa attaactatt gttaacaatg ctgtttcaat aagttgagct tgatataaag
     8641 gtgccttaac agtaattaat ggctcattag ggaacatgat agttccttcc tcaacagcat
     8701 atatatctcc tgtgaattta aattctttta aaaattttaa gaacttgtca gaaaataaat
     8761 ttaagttttt taagtactct aaatcttcat ctgaaaaatg aagattatct atgtattcaa
     8821 caacctgatc aataccacaa actatagtat accctccatc acaagcgttt tttctaaaaa
     8881 acatatcaaa tacaacaatg tcttcatgaa tattcttttc aaaatagcca tttaacatag
     8941 taagctgata taaatcagta agaagtgtta gatttctcat tatttctctc cttaattatt
     9001 attaaatatt aaaaattgta aaaactccca cacttatact aaaacataat atagtacaac
     9061 tttcaagtat aaagtattac ctaactatca taaaattatt taaagaggag gatgattata
     9121 ataggaaaaa tatggtttta tatggtatcg ataggaataa taggaagctt gatttctaaa
     9181 aatatgggtg aattaaataa agttatatta agtgaaacat caaaaggtat agagtttgca
     9241 ataagtctag ctggtatcat ggcactctgg atggggataa tgaatatcgc aaaagaatca
     9301 ggtttaattg aaaagatagg tcaaaaacta aatcctataa tgagaagatt atttccttct
     9361 attccaaaag gtcataaagc aatgtcttat atagttatga atattgcttt aaatatgttg
     9421 ggtgctggaa atggtgcaac agcatttggt ttaaaagcaa tgagtgagct tcaaactttg
     9481 aataacaaga aagatacagc cactaatgat atgattatgt ttatggtaat caatatatca
     9541 tctatacaaa taattccatt tactataatc aaactaagga tggatatggg ggctcaaaat
     9601 ccaagtgaaa taatttttac gactttattt gcaaccataa tatctacagc agtagcaata
     9661 ataacatgta aattttttca aagaagatat agataaaggg gatgtagata catggagata
     9721 ttttcaaact tacttatacc aataataatt ctgtacatag tagtttatgg taaatataaa
     9781 aaaatagatg tgtatgatag ttttgtaaaa ggagcaattg atgggttaaa agcagcatgg
     9841 gatatcctac catatataat aggtatattt ttggcaatag gaatattcaa aactggtaaa
     9901 ggtcttgata tgttagaatg gctatttaca ccaatagcaa atatgatgag tataccaaaa
     9961 gaattaatag gtctaataag tgtaaaacct ttatctggaa gtggtgcttt agggatgtac
    10021 agtgaacttg caaatagagt tggaatagga agtttagtag aaaaaatggg tgctacgatt
    10081 gtaggttctt ctgagactat tttttacaca atggcaatat actatggaag tctgaagata
    10141 aaaaatacta gacatacatt atcatgtgct atgattagcc atgttgcagg tgtcatagca
    10201 gctgttttta tttgctatgt aatatttgta taatttgttg acaatatggg aaaaatttta
    10261 taatatagtg attatataga aacaaatagt aatacaaatt acaagctatg aaaagaagag
    10321 taaataaagg ttttttctat cagagagcta ggttaggtga aagctagtgt caaaagcttt
    10381 attgaagatg gtcttggagc tttttatctg agcaagtgtg ctagggtaaa acggttggac
    10441 acgttatagt ctactaaggt atctataaaa tgtttctaca tttcatgtga aacataacta
    10501 ttatagatga attagggtgg taccgtgaat aatctcgccc ctatgttaac tcataggagg
    10561 cgatttttta ttatttaaat gaaagaatca tatgattacg aacgataaaa aattaggagg
    10621 taaataacat gagcaaaccg agtttttatg taacaacacc aatatactat ccaagtggag
    10681 gattacacat aggacatact tattcaacag tagctgcaga tacaatagct agattcaagc
    10741 gtttctgtgg atatgatgta aagttcttaa caggaacaga tgagcatggt gaaaaaattc
    10801 aaaagaaagc catagaacaa ggaatgtcag agatagagta tcttgatgga atgataaaag
    10861 atataaaggc cttatggaat actatggata tatcttatga tgattttata agaactacag
    10921 aaaaaagaca tacagacata atacaaaaga tatttactaa actatatgag caaggtgata
    10981 tatataaagg tgaatatgaa ggtagatact gtactccttg tgagagtttc tggacagaat
    11041 ctcaattact agaaggaaat aaatgccctg actgtggaag agaaacatat ttagtaaaag
    11101 aagaatctta tttctttaga ttatctaaat atgaagacag attaaaagag ttatttaaag
    11161 atgatagttt ctgttttcct gctgctagaa aaaatgaaat ggtggcaaac tttttagaca
    11221 aaggattaga agatttaagc gtaactagaa cgacttttga ctggggtata aaagttcctt
    11281 ttgatgaaaa acatgtaatt tatgtgtggg tagatgcttt atgtaactat ataacagcat
    11341 taggttatat gactgataat gatgaagaat ttaaaaaata ttggcctgcc aatgttcaaa
    11401 tagtaggtaa agaaatagtg agattccata caataatatg gccagcgctt ttaatggctt
    11461 taggtctaga agttccaaaa caagtatttg gacatggatg gatactattt gctgatgata
    11521 aaatgagtaa atcaaaaggt aatgtggttt atccagaacc tataatagaa agatatggaa
    11581 tagacacact taagtacttc ttattaagag agtttgcatt tgggcaagat ggaagttata
    11641 ctcatagaaa ttttgtaacg agaataaatt atgatttagc aaatgattta ggaaacttaa
    11701 taagtagaac tgtagctatg gttgaaaaat acaataatgg tataatacca acagcaaaag
    11761 tttcaactaa ttttgatgct gacttaaaag aacaagctgt ttcaactaga gaaaactttg
    11821 aagctgaaat ggataaaatg caattccatg aagcattaga gagtgtatgg aaattagtta
    11881 gaagaactaa caaatatata gatgaaacta tgccttgggc actagctaaa gatgaaacta
    11941 agaaagatga attagacaca gttttatata atttatgtga atctataaga ataatcgcaa
    12001 cactaataaa tcctataatg aatgagactg ctaataaaat atatgaacat ataggaataa
    12061 aaggtcaaga tgatataact acatgggaaa gtacaaaaac atttggtctt attggagaaa
    12121 atgtaaaagt atttaaaggt gaacctttat tcccaagatt agatgtagaa aaagagatag
    12181 aagaattaac taaaatgttc tctgggaaac ctccagtaga agaaaaacct ttagagcata
    12241 aagaagaaat tactattgat gatttagaca aaatagagct tagagtaggg aaaataataa
    12301 gctgtgaaaa acatccaaaa gcgaataaat tattagtatc acaagttaag attggaccag
    12361 aaacaagaca aatagtatca ggaatagctg aatactataa gccagaggat ttagtaggaa
    12421 aagaggttac agtggtatgt aacttaaaac cagttaaatt aagaggtgta gaatctcaag
    12481 gtatgatatt agcagcaggt gatgatggag acccatatgt tttaccattt acacaagggg
    12541 ctaaagatgg atgtgaggtt cgttagtagg taattaaata gtaaggagat taaagatgtt
    12601 atttgactca catgcacatt taaatgatga aagttttgat gaagatagag atgaacttat
    12661 tggttcatta aaagataaag gtgtagattt ggttgtaaat ccaggagcag atatagagac
    12721 gtcgataaca gcaattgaac ttgcgaaaaa atacgacttt atatattcag cagttggggt
    12781 acatccacat gatgtatcaa aactagatga tacagcaata gaaactctaa gaaaacttgc
    12841 aactgagaat gaaaaagttg tagctattgg agagattgga ttagattatt attatgatta
    12901 ttctccaaga gaagaacaaa aagagtggtt taagaagcaa attgaattag ctaatgagct
    12961 aaaacttcca ataataatac atgatagaga tgcacatgga gatacttttg agataataaa
    13021 gaatactaaa aatcctgaaa taggatgtgt acttcattgt tatagtggga atgtagagtt
    13081 agctagagaa tatgttaaga tgggttgtta tatatcaatt ccaggtacgg ttacttttaa
    13141 gaataataaa aaaactagag aagtagtaag agaaatacct ctagaaagat tattcataga
    13201 gactgattca ccatatatgt caccagagcc acatagagga aagagaaaca atccttctca
    13261 agtatctttt gttgctgata agatagccca ggaaaaaggg atatcttatg aagaagtatg
    13321 tagagttaca aaagaaaatg caaagaaatt ttttaatata aaatagacaa attaaatctt
    13381 ataatgatat atataataag tcattataag tataggatga gagtgcctca aaatgagttt
    13441 tttagtttat gaggcactct ttttgtatga aatcaaatat attttcatca tttcaacaat
    13501 gaaaaagaag tttatcatat tttgagatag tttgttttta tttatttaat tcatatttta
    13561 aaatatattt tcttaacatt gttaaaaaag aatttacaag atattcatta gaattaactc
    13621 aactctaata ttcacttaac ttaagttgtt tattatatta ttaaagagaa gtaaacgaaa
    13681 agtagattaa ggaggttgaa ttttgtatac agttgatagt actaagatat tgataaatat
    13741 gaacttagag aaactagaaa agatatactc aatgataaaa gatacatcaa gtatttctat
    13801 aattaaagaa cctaacttgg ctacagtaat ggtaagagct aatgaaagtg taaaaaacac
    13861 aacatttaat ttaggagaga tattagttac tgaatgtagt gtaaaggtag atgaaagctt
    13921 gggttacgga atagtttcag aaaacaataa taagaaagcc atttatttag cagttataga
    13981 tgcagtttta catagcaata acagtaaatt tgatgagtta aagaattaca taaataaaag
    14041 tgtatatgaa gagaacttaa ggtatgaaca agagattatt gatgaattta gcttgattaa
    14101 taaaacgaaa gttcaattca atgctatgga ttaaatagga ggagaatcat ggtaaattat
    14161 acacaagaaa tatatagaaa gatgctagat agtgcttcat ttcctgggaa attaaataat
    14221 atagataata taaatataga aaataataca aaactattga atggagcaat tggtatagca
    14281 atcactttat tagaccaaga agttaccttc tatatagaaa attatactca aaaagatata
    14341 aaaaatataa gagcacttac aatgtcaaat caaatagatt atagaaaatc agattatata
    14401 tttttagata ttaattcaga agtggatata ttacagatta aaataggttc ttttgaatat
    14461 cctgatgaaa gtgcaactat agtacatcaa gtaaatgatt tatcaacgta ctctcaaact
    14521 aagtatataa agcttgattt gagtggtcct ggtataaaat ctaaaaatag tatatatata
    14581 aatggaatta gagaagaatt tatagaaaga ttatctagca taaataaaga ttatccaata
    14641 ggaatagatt tgattttagt agataaaaaa ggagaaattg catttatacc aaggtcatca
    14701 aaattaagtt gggaggtatt atagaaatgg cttatgtagc agttaaaggt ggagaagaag
    14761 ccatattaca ggctaaaaat atacttgagt tttatagggt aaaaggagat agcttgccta
    14821 tagatgtaaa gcagattgag gaacaaatgt cttatgctgt agacaaagta atgtctgaag
    14881 gaagtttata caacaaggaa ctatcagcat tggctataaa acaagctcaa ggtgatatat
    14941 tagaagcctc tttctatgta agagctttaa ggtcaacaat gcctagaatt acatattcag
    15001 aacccataaa tacagaagaa atgagaataa aaagaagaat atcagctact tttaaagata
    15061 taccaggagg tcagtatctt ggggcaacta gagactatga aaatagaata ttgaatgtag
    15121 aattattaga agaagatgaa aaaaagaata gacattttag agatagtttt ataaatgaat
    15181 tagaaacagt agatgaaatc cctgaattta caaaaatatc taatatattt agaaaagaaa
    15241 atttattaca agaagaagtt tgtgaaaaca aattggaaga agttattgat gatattacaa
    15301 gaaatagttt aagatttcca gtttctaggt cagcaaagtt acaggcatta actagaggtg
    15361 aaagtggagt agtgtcaggt tttgcttatg ctattgtaag aggttttggt gatgaacatc
    15421 catatataag tgaacttaga actggtaatg tatttataaa agtaaaacat cccatctatg
    15481 aagatgaaga aataataata ggggatatgc tggttactga atgtgaaata gtttcaaaag
    15541 catcacaaaa taagactgaa aattctaaaa ctaattcgga agataaaaat aagatgtctt
    15601 taggatatgg tctttgtatg gggaataatg agaataaggc tatatctatg gccatactag
    15661 acaaatcttt ggagccaaat agtgaaaaac tagcacaaga tgaagaattt gtattgcttc
    15721 atatagatgg aatagattca ttaggattta tatctcactt taagcttcct cactatgtag
    15781 attttaaagc ggatgtagaa agaataaaat agtttgagga ggtaagttta ggtgatatat
    15841 aactatggtt ttttagacga aaacacaaaa aaagaaataa gaagaaagat attaaaagca
    15901 atatcaatac ctggttatca agttcccttt ggttcaagag agttgcctat agctaaggga
    15961 tggggtactg gaggtttaca attaacacta tcattaattg gtaaaaatga tgtagtaaaa
    16021 gtaattgacc aaggaagtga tgattctaca aatgcttgta atataaggag ctttattgca
    16081 agtgtaagta atgtagaaac cacaaaagat acattagaag ctacattgat tcaaacgaga
    16141 catagaatac cagaagaaaa acttaaatct aatcaaatac taatatttca agtaccaata
    16201 ccagaaaccc ttagaatagt agaacctagt gaagtagaaa caagaagaat gcattcagaa
    16261 gaagattata gtaggatgtg ggtatatctt tatgaagata tagttagatt taatgatata
    16321 tctatagcag ttgaatatcc atgtaaagta aatgatagat atttaatgaa tccatcacct
    16381 atacctagat ttgatataaa aaaattaaat atgtcagaca atttattctt gtttggtgca
    16441 ggtagagaaa aaagaattta tgctattcct ccatatacaa aggtagaacc tttagagttt
    16501 gaggattata agtttgaaga ggaaaagttt gaaggaaaat actgtagttt atgtaacagt
    16561 acaaatacat ttttagatga agtatatgac agtgacacta atgaaaagta ctatagttgc
    16621 tcagatacaa gttattgtga aaaagttaga ttaaaaaata acagtataga tgtgacaata
    16681 ggaggtactt ggaatgagta aaataaatga ctctaaaaga ttagaaaata tagacaagca
    16741 agtattaata ggaagaaata tcaatcttat ttatggagat gggtgtgaaa agtgttttga
    16801 aagcacagga tatgaaagca ataatatatg taagtattgt ggttctatag ttgcatgtaa
    16861 taatgtaaat ttagaacttt atgaaggaga agtccttgga attgtaggtg aatctggttc
    16921 aggaaaaagc actttattaa aaatactgtt tttccaagaa catgcaaata gtggagaagt
    16981 atatatatcc cattacaata aagacacaaa tatactagag ctttcagacc aaaaaaagag
    17041 atacataaag aatcatttta tgggtattgt gtatcaaaat ccccaccttg gtttaaattt
    17101 agactttagt agtggtggta atatagcaga aaaattattg atggcaaatt tatataatgt
    17161 tgaaaaaata agagatagag gcaaagaact tttaataaaa actaatatac ctgtagaaag
    17221 aatagaccat aaaccaaaat actttagtgg gggaatgcaa caacgagttc aaatagcaaa
    17281 agctctttca aataaccctc caatattgct attagatgag gttacaacag gtttagatgt
    17341 ttctgtgcaa gcggaagtat tggatttaat aagagaaatc caaagagaac ttaagatttc
    17401 tatgatagtt gtatcccatg actttgatgt tataaagatg cttgctgata ggacaattgt
    17461 aatgaaaaat ggaagtgtaa ttgagtctgg tcttacagac caaataatgg aggacccaca
    17521 acacccatat actcagcaac ttataaattc attgctataa atatcgtttg taaataagca
    17581 aaatgggggt ttttaataaa atgaaagaaa ctatacttga agtaaataat tttagtaaag
    17641 actttgagtt acatgcattg aacaagacta taacggcatg tagtaatata agttttgaag
    17701 tttcaaaagg tgagttttta ggaataatag gtaaatctgg agcaggtaag tctaccatat
    17761 taaaaagtat ctataaaaca tatataccaa caactggaga aataattttt aattcagaaa
    17821 tatatggtaa tgtagacctg gctaaaattg gagaccgaga aatgataaat cttagaaaga
    17881 aagaaatagg atatgtatca cagtttttaa agacacttcc acgaataact gcaattgaac
    17941 tggtagtaca ttctttaatt gagagtgggt ttgaaaaaga ttgttcgtat gatatggcaa
    18001 aagatatact taaccaattt gaaataaagg aaaatttatg ggatgcgtat ccaaataatt
    18061 tttctggtgg agaaaaatta agattaaatt tagctcaagc tatggttaaa aagcctagac
    18121 ttttgttact tgatgaacca actgcatcac ttgataatca atctaaaata tatgtaaaag
    18181 aaaaaatgtt ggagcttaaa agtcaaggaa ctacaatgat aggaattttt catgatatag
    18241 agtttatgga aactgttata gataagacct ttactatgac aaaagggact ataagtgaaa
    18301 gtggggtagc ctagatgata aaactaattc ataatgcaaa tattgtatta gaagatatga
    18361 ttttagaaaa tggtcattta atcatagagg gagatacaat aaaaaaagta tcaaatgata
    18421 agattgatta cgcatttgac tttaatgaga tagatgaaat aatagatgca aaaaacttat
    18481 atgtaatacc tggtattata gatattcaca gtgatgccat tgaaaaggaa atagagccga
    18541 gaccaagtac attattacca tttaatatgg cattttatga actagataaa aaactacctg
    18601 caaatggaat aactacagtt tatcattcta tatctttagg ggatggagta ggagttagaa
    18661 gtattgataa ctctttaaag atgattaaaa atattgattc atataaaaat atagatagta
    18721 agagcataaa tcataaagtt catttaagat atgaagtgtt gtattatgaa ggtttagaaa
    18781 aggttttaga actattagat gaaaataaaa tagactactt atcaataatg gaccattctc
    18841 caggacaagg tcagtataca aatccaacat tttataaaga gtatgctact aaagtgtggg
    18901 gagttacaga aaattatgta gatacatggt tagatgattt agtaaatctg catgataatt
    18961 tagattggaa taaaatagct aatattatag gtatagccaa aactaaaaat ataaatgttg
    19021 catcacatga tgatgataca ttagaaaaaa tggatttcat aaaaaatata ggcataaatg
    19081 tatctgaatt tcctataact cttgaagttg caaaacattc aaagaaattg ggtgttctaa
    19141 cttgtttagg tgcaccgaat attgtaagag gtaaatctca taataacaat ttaaaagcaa
    19201 tggatgctat attggaagat tgttgtgaca ttgtgtgttc ggattatctt ccatcagcta
    19261 tgataaagtc tatgtgtata gttgcagaaa gaataaataa tttaaataaa gcagtcagtt
    19321 tatttacctc aaatcctgct aaagcagttg gtatatatga tgaaagaggt agtataaaag
    19381 aaaataaaaa agcagattta gttttagtag atattgatag tgaatatcca aaggttgtaa
    19441 atactattgt aaatggaaaa acagtttata aaagggaggt ataaagattg ctaacagagt
    19501 tacattgtca tacaagtata tcagataata attttactac tgaagaaata ataaaaaaag
    19561 cagcagaaaa gaatataaat atcttagcaa taacaaatca tgacacatta tctggattgg
    19621 atgaagctct tgtgcttgga gagcattatg gaataacaat aataccaggt atagaaattt
    19681 cagcctatga ttataaaaat agaaaaagag ttcatattct tggatataac atagatttaa
    19741 acagtcaaga aataaaaaat ttgtgcaatc caatggtatg tgacagacat aaagcatctg
    19801 ttaagatggt aaataaaatt atagatttag gatacaaaat atctctggaa gatgtaaaga
    19861 aatattcatc aaagactgga atatttaaac aacatataat gaaagctctt atagataaag
    19921 gatatacaga taatatatat tcaaatctat ataaagaatt gtttcataga ggaaacggca
    19981 aggtatatgt atctttaaaa tatgtggatt acagagatgc aataaaagct ataaaaaatt
    20041 ctggtggaat ttgtgtgtta gctcacccgg gtcaaatgga taattttagc gctattgaag
    20101 agatggtaaa agtaggatta gatggaatag aagtatatca tccatctcac aacaaagaac
    20161 tagaaaaaga gtcgctttct tatgctaaga agtataatct agtggtcaca ggaggttcag
    20221 attaccacgg attttattct caacataatt atgaacttgg aagcaagagc ttaaataaag
    20281 aagatttatt taagtttagg ttagctttag gaggggtatt ataaatgtct tttttacaag
    20341 taaaaaatgt aggtaaaagt tatggacaag ttaaggtgtt aaaagacata tctattgata
    20401 ttgaaaaagg agagtttatt tgtttactag gtccaagtgg ttgtggaaag agtacactac
    20461 ttagaataat tgcagggctt gaggataaac atggtggaaa aataataata aatgataagg
    20521 atatgactaa ttcaccacct gaaagtagaa attttggtat agtttttcaa tcatatgcat
    20581 tatttccaaa tatgaatgtt tacaaaaaca tagcctttgg actagaaaat aaaaacataa
    20641 gtaaatctaa tattgataaa aaagttaaag aagttctaga agttgtagaa ttaagtggat
    20701 atgagaaaaa atatccatca caattatcag gtggacaaca acaaagggtt gcattagctc
    20761 gtgcgattgc tttagagcca gactttttac ttcttgatga acctttatct gcattagatg
    20821 caaaagtaag acttaaactt agggagcaga ttagaagttt acatagaaaa ctaggcatta
    20881 caactataat ggtaacgcat gaccaagaag aagctctttg tttagctgat aagatggttg
    20941 ttatgaatag aggagaaata atacaagttg gaacgccaaa ggaggtatat aaaaatccag
    21001 aaacaccttt tgtagctgat tttataggaa caataaattt tatagatgat ggtattaata
    21061 agatagctat tagaccagaa gatataaaag tagagtctaa tagagattct aaagataagg
    21121 atataaaagt gggagaaatt ttagatatcg aatttagagg atttaactat agaattacag
    21181 ttgagtatcg ttctaagcaa atgaaattag atgtagtttc caaagttgca gaacaaatga
    21241 aactgtgcat aggttctaaa ataaacttta agatacctaa agaaggaatt gtacaatata
    21301 aatcagaggg atgtgcttaa tagctagggg tgaataaatg ataacatcaa aaaataaaag
    21361 aagaataagt gatagcacaa tacaaaaata tttcttgatt ttttcggtaa ttgtactaat
    21421 agtatttata ttatttccat tagtatcatt attaaaaaat gcatttatgg atacaagtgg
    21481 aaattttata ggaatttcaa actttagtaa atatgttgaa aatccaagtc ttatagcatc
    21541 atttaaaaat tcaatttttg tatctacagt ctcatcttta atatcactat gtttagcatt
    21601 tatatattca tacgcaataa caagaacaaa cataaagttt aaagaatttt ttaaaactat
    21661 gggaatgttt ccactttttg caccaactat gttatatggt atttcactta tatacctttt
    21721 tggaaataag ggaatcttta caaccatggg tctggaagta ccactatatg gtccattagg
    21781 aataattatt tctcagattc tatttacatt tccacaagct tttttaatat tatctgtagc
    21841 actttcgatg gctgattaca gactttatga agcagcagat agcttgggtg ctagtgaatt
    21901 taagaaattt tgcaaaataa caattcctgg tataaagtac tcattactta gttcattctt
    21961 tgtatccttt atacttgcat ttacagattt tggagcagct aaggtagttg gaggaaacta
    22021 caatgtactg gctactgaca tatataaaca agtagttgga caatttaaca taccaatggg
    22081 agctactgtt tctatggtaa tgttaattcc tgtactaata gcatttacat tagataagat
    22141 ggcaagtaag aaacaaggta tgaccataac tgcaaagtct atgccatata agattaagga
    22201 aaataagttt agagatagag tatttttaat agtatgtagt tttataacat tgctaatttt
    22261 tattttaata ttcgtttcag ttataggttc agttataaag ttatggccat ataatttaag
    22321 ctttacaatg gaacactata catttaaagg tattttggga agtggaataa aaacatattt
    22381 aagcagtttg aaaatatcaa ctttaacagc aatatttgga actatatttg tattttttag
    22441 tgcttatatg atagaaaagg tggataagtt tcctaaatta agacaaacag cttacctttt
    22501 atcaatgata ccaatggcct tacctggctt agttttaggt atatcatata taactttttt
    22561 taactcagca agtaatccat taaatttttt atatggaagt acagcaatat tagttatagt
    22621 aaatgtagtt catttttatt cagtttgttt tataacctca aattcatctt taaaaatgtt
    22681 ggataaagag tatgaattag ttgcaaaatc cataaatatt ccattctata aagtattttt
    22741 taatataaca gttccaatgt ctataacatc aatacttgaa atagtagtat attattttgt
    22801 aaattcaatg gttactgttt cagcattaat atttttatat acacctcaaa ctcagactgc
    22861 atcaatatca atactaaagt tagatgagat aggatatata ggaccatcag ctgctatggc
    22921 agtattagtg cttttaacaa atataatagt aagacttttg tacgaatttg taacaaagaa
    22981 attaaaaaat aacacacaaa aatggcagca aaaagatgtt gcatagggga ggtaaaaatg
    23041 aaatttaaga agctagtttc tttaggagta ttatcaacac ttttattaag tgtaataaca
    23101 ggatgtagta ctgattcatc aggttcagat tcaaagtctg atagtaagac aaaaggggat
    23161 ttaacagttt acacagcaat tgaagaagat agtatagagc catatttagc tacatttaaa
    23221 gagaaatatc cagatattaa attaaatatt gttagggcat caacaggaga tataacagca
    23281 agattattag cagaaaagga taatcctcaa gcagatgttg tatggggagt tgcagccaca
    23341 agcttattgg ttgcagatga ccaggggatg ttagaaccat atgctccaaa aggttctgaa
    23401 gaaatagagt ctgcatttaa agatgataaa gaagttcctt cttgggttgg gatagatgct
    23461 tggatgacag gtataacagt aaacactaaa gaattaagtg ataagaacat cccagttcct
    23521 agttcttacg aagatttaat aaagccagaa tataaaggtc ttatatctat gcctaatcca
    23581 tcttcttctg gtacagggta cttaacagta tcagccctta tacaaattat gggggaagaa
    23641 aaagcatggc agtatatgga taagttacat gagaatattg gtgtatacac tcaatctggt
    23701 tcagctccag ctacatcagc agcttcagga gaatatccta ttggtatatc ttttggatat
    23761 agaggtataa agttaaaaga ggaaggctat ccagtagaag tagtatttcc aaaagaaggt
    23821 tctggatggg atatagaagc aaatgctctt gtgaaaaaag ataatataaa agaagcatca
    23881 aaagtatttt tagattgggc tataagtaaa gatgcaatga acgaatactc taaaaactat
    23941 gcagttacta ctatttctac aggaaatcca ataccagaag gattccctaa aaagccatta
    24001 gagcaaatga tagataatga tttaaaatca gcagcaaaaa atagagaaga tatactaaat
    24061 aaatggatat caaaatatga tggtaagaca gaaaaagaga gttaagtaaa actatacaaa
    24121 tggagtaaaa agaaaaattt tttttaagta gattttatta ttataatata agttgagaat
    24181 aatataggtc aagaaaagtg aatattctat aaaaatgtat agagta
//
LOCUS       DAEOSP010000039        24011 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.039, whole genome shotgun sequence.
ACCESSION   DAEOSP010000039 DAEOSP010000000
VERSION     DAEOSP010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24011
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.039"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            247..3150
                     /locus_tag="KRH54_001293"
     CDS             247..3150
                     /locus_tag="KRH54_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8578960.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEEDEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKISDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDESLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            3183..3629
                     /locus_tag="KRH54_001294"
     CDS             3183..3629
                     /locus_tag="KRH54_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8578961.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGDMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            3752..4054
                     /locus_tag="KRH54_001295"
     CDS             3752..4054
                     /locus_tag="KRH54_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8578962.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            4235..5641
                     /locus_tag="KRH54_001296"
     CDS             4235..5641
                     /locus_tag="KRH54_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG8578963.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPVITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKMKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            5985..7043
                     /locus_tag="KRH54_001297"
     CDS             5985..7043
                     /locus_tag="KRH54_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG8578964.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            7142..7828
                     /gene="araD"
                     /locus_tag="KRH54_001298"
     CDS             7142..7828
                     /gene="araD"
                     /locus_tag="KRH54_001298"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBG8578965.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEIRNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            7846..8493
                     /locus_tag="KRH54_001299"
     CDS             7846..8493
                     /locus_tag="KRH54_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBG8578966.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(8666..10033)
                     /locus_tag="KRH54_001300"
     CDS             complement(8666..10033)
                     /locus_tag="KRH54_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBG8578967.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(10223..10311)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10739..12859
                     /locus_tag="KRH54_001301"
     CDS             10739..12859
                     /locus_tag="KRH54_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBG8578968.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLKSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            12963..14216
                     /locus_tag="KRH54_001302"
     CDS             12963..14216
                     /locus_tag="KRH54_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBG8578969.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(14370..15431)
                     /gene="ytvI"
                     /locus_tag="KRH54_001303"
     CDS             complement(14370..15431)
                     /gene="ytvI"
                     /locus_tag="KRH54_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG8578970.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            15705..16445
                     /locus_tag="KRH54_001304"
     CDS             15705..16445
                     /locus_tag="KRH54_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBG8578971.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILRELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            16457..18235
                     /locus_tag="KRH54_001305"
     CDS             16457..18235
                     /locus_tag="KRH54_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG8578972.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            18402..19319
                     /gene="pfkB"
                     /locus_tag="KRH54_001306"
     CDS             18402..19319
                     /gene="pfkB"
                     /locus_tag="KRH54_001306"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG8578973.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            19356..21275
                     /locus_tag="KRH54_001307"
     CDS             19356..21275
                     /locus_tag="KRH54_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8578974.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVTEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
     gene            21871..23397
                     /locus_tag="KRH54_001308"
     CDS             21871..23397
                     /locus_tag="KRH54_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG8578975.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKDIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            23425..23982
                     /locus_tag="KRH54_001309"
     CDS             23425..23982
                     /locus_tag="KRH54_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8578976.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
BASE COUNT         9628 a         2514 c         4208 g         7661 t
ORIGIN      
        1 caagtttttc aatcataaat ttataaagta ttttgcatta aaatttaaac aaagaattaa
       61 attatttaag attatgaata actaatacat ttagtaaatt actaaatata caaaatgtaa
      121 atacttatca aaatgtataa atacttatga catctagtta cttacagggt ttaagtagaa
      181 aaaaatatca aagtaagtat ggcatgatag ttgcttatat atttatcaag aaaggagtta
      241 ttaagcatgg agttgagaga taaacttgta aacatcataa atacagaaaa taaaaaagat
      301 ccattaacag atgttcaaat agcaaagttt ttaagtacaa ctagagagaa cataactaat
      361 ttgagaaaag aacttaatat aagtaattca aggcaaagaa gatatcctta tttaaaatca
      421 gcaatttcgg caatattaca aaagaataag aatatttcta tatcagaaat tacaagggaa
      481 ttgatgacag aaggctttaa tatatcgaga agagttgtgg aggaactttt acctaaagaa
      541 agtttagaaa tggatgtagt agaggaagat gaagaagaat cgaatgaccc atttgaaact
      601 ctgataggta ataaagggag tcttagaaat gctgtagagc aggctaaatc tgctatattg
      661 tatccaccaa aagggttacc aacgttgatt attggggaaa gtggagttgg taagagttta
      721 ttttctagac atatgtatga gtttgcaaag caaaaaaaga tagtaaaaga gagctccaat
      781 tttgttgtat ttaactgtgc tgattatagt gacaatccac aattattatt atctttgtta
      841 tttggatata aaaaaggtgc atttacaggt gctgaatatg atacaccagg tcttgtagaa
      901 gaagctgatg aaggagtttt atttttggat gaaatacata gattgcctcc aaaaggtcaa
      961 gaaatactat tttctatctt agatagggga aagtttagga gattaggtga aactaatgct
     1021 gaaagaaaag taagtattat gttgattggg gcaactactg aaaatgtaga gactaattta
     1081 ttattaacat tcagaagaag gataccaatg ctaataactt tgccaccatt acatgataga
     1141 ttactaaaag agaaaataga ccttatatac aatatctttc aacaagagtg taatagaata
     1201 aatgcaaaaa tatttgtaga taaaaatgtg attgaaatac ttgcactaaa aaagtttagt
     1261 ggaaatatag gacaattaca aaatatgatt caggttcttt gtgcaagagc ttttatgaag
     1321 tttataaatt ccaaagatga agatagtgaa tctatagtag tagtagatat aaatgaagtt
     1381 ttaaagctta aagattcgtt taaagatgct gcctttcaag agattgaata tacagagata
     1441 agaaaatatt tgaaaaatgc tatatttata ccatttaatt tagaagaatc atcagtaaaa
     1501 ggaagtcttt taagtgatga atatatatta ccagaggaca tatacaaaac aattgaaaag
     1561 aaatattatg acctaaaatc cctagacatt gaagatatag atatagagaa tattttatgg
     1621 acttttattt taaatagatt tgctgactta aagtttaatc ttgatagtaa tgaagaaatt
     1681 ttttcaatga gtgatttaaa tagttttgta agtgatagtt tagttaaact ggtaaaagaa
     1741 cttatgaatg atatagttga aaaaaatatg aatttagaag taaataaaaa tatgttcaaa
     1801 tatctagcaa tacacttaga agaagctata aaaagaatta agctaaatca aaagataata
     1861 aatgtaaatt tagaaaaaat aaagattgac ttttcaaaag aatatgaaat tagtaaaagt
     1921 tttgcaataa aaattgagga atcaattggt attaagattt cagatgatga aattggattt
     1981 ataacgctct acataaagtc tgctctaaaa aatgaagtga aaaaagataa agtagggtta
     2041 ataataattt ctcatggacg tatagctaca gagactgtaa atgtagttaa agaactttta
     2101 ggtgtaaagt ttcctgtggc tatagatatg cctttagatg aaaaaccaat aaatatatac
     2161 aataaggcag tagaattgtc aaaaataata gaccaaggga aaggcatatt gtttttggtg
     2221 gatattggtt cacttacaaa cataggtcaa atagtaaata agagaaccaa tataagtact
     2281 aaaactattg atagagtaga cctcctaatg gcactagaag caactagaaa agtttctatg
     2341 ggagaggaag aacttgatga aatatttttt tctttgctaa aagataggat gggatataac
     2401 tatgaattag aaaaacacat agataaatca aatgcaatta taacattatg tcttactgga
     2461 gaaggaactg ctaagtatat aagtaaaacg ttggaagaaa agtatgataa cactaaatgt
     2521 tatcaaatga gtgcattgga tgaaagttta ttttgtaaaa ttgaaaatct aaaagagaca
     2581 aacaatatat tagctattgt tgggacaata aaccctaaga taccaggtat aaattttata
     2641 ccctataata aagaactttt caaaaatttg gatatctatc tatcaaaatc aaaacaacaa
     2701 gatgacagac caattaaatc gtatgaaaga atgcttgatg aggatttagt gatttttgaa
     2761 ccagatatat attttaaaaa ggatttatta gaatatgtat gttcaattct tataaataaa
     2821 gaatatgtag aaaaagatta cttagattct gttttacata gagaagaaat gcttccaaca
     2881 tattctaaag gagctatagg tgtgcctcat ggagattctt ctactgtaaa tagtacaagg
     2941 tttgtttttg ttaaactaaa gaatcctatt gattggggtg taggtaatgt aaatttcata
     3001 tttatgccag tttttcaggc taatgataaa gagattgtta aaaatgttct tgagatacta
     3061 aaagatgcag aatttatgaa caatgccaat aagtgttttg ataaagatgc ttttagaagg
     3121 attatatttg ataaatttaa acatctgtag ccatagcaaa aaaattgaga ggagttagag
     3181 taatgaatgt agatttaata tcattgggtt tagaatctaa tgattatgaa agtgttattg
     3241 aagaattagg aagtataatg tgtaaaaaag aatatgtaaa agaaacatac ataaatgctg
     3301 tcttggaaag agaaaggaca ttaccaacag gtctagatat tggagacatg tgtgttgcta
     3361 ttccacatac agattcaaag catgtaaatg agtctaatgt agctgtaggg atattgaaaa
     3421 atcctgtaaa gtttaattca atgatagacc caaaagatag acttgatgta gaacttgtat
     3481 ttttattagc agtaaagaac ccagactcac aagtaaaact gttaaaagat ttaatgtcag
     3541 tttttcaaaa tataaagtta ttaaaaaata taaaaaacgc atcaacaaaa gaagaagtag
     3601 ctaaattatt ggattttata gaaatttaat gtaggagaat tttataaata aaaaattaaa
     3661 atttaaacaa tcaaagtaag tattattttt ttaataggtt taatcttata taaaataaaa
     3721 atttagagtg aaaaattagg aggaaattat tatgaaacaa tttaatgtat tatcagtttg
     3781 tggttcaggg acagttacat catctatggt agcaggtaaa ttaaaagaag tactaggtaa
     3841 taaggggatt tctattacta caacagaagc tagaccaaca gaagcactaa atttagcaca
     3901 atcaggaaga tttgacttta taacatttac gagtccactt caaacaggcg attatggaat
     3961 accaactata aatgcatttg cttgcctaac tggtataggg gaagaggcat tctttgaaga
     4021 agttttgact gttattgaag ggatagataa gtaagttttg gagacgtgaa tttatagtat
     4081 ttaaatcaat gatttagata ctataataag ctaatattaa ctaaatattt gtggaaaaaa
     4141 cattttatat gttgtcataa attaaaaaat tataaaaata gtcacttaag tattaaatta
     4201 agcttataaa tatttataag taggagggaa aaagatgtta gaatttatga aatctataat
     4261 cgatacattt ggttctgcaa ttatagttcc aattataata ttcataatcg caaagatatt
     4321 taaagtaact actaagaaat cgtttctttc tgctgtgtat gcaggagttg ctttacaagg
     4381 ttttacactg attttaaatt catttacacc agtaataaca ccagttatca ataggatggt
     4441 tgagagtaca ggagtaaacc taccagtttt tgatgttggt tggcaagcaa catcattggt
     4501 tgcattttca acaagtgcag gtatgatata tttaggtctt ggtatattat tacaaacaat
     4561 tttatttttg ataaaatgga cagatgtgtt tcaaccatca gatttatgga ataactattc
     4621 ttatatggta tggggagcaa tggttatagg agtaacagga aattttcctt taggtatagc
     4681 atgtatggtt ttattaaacc tatatagttt attaatatca gagttagttg caaagagatg
     4741 gtctagttac tatagatatc ctaattgtac aataatagct atgcataatg ttgaagcttc
     4801 tgtgtttgca gtatttgcag accctatata caataaattg ggattaaata aaatgaaatt
     4861 aaatccaaaa gaattagaga agaaattagg gtttttaggt gaaccaatta ctttaggtct
     4921 ttttttaggt atgtttatag gtatattagg taatatgacg agaataaata ctatggaagc
     4981 ttggggagaa ataatgaaag taggtatatc tacaagtgca gtgatggcaa ttttccctaa
     5041 agtagcaagt atgtttgctc aagcatttgc acctataaca gaagcagcta gaaagattat
     5101 gcaaaaagct ggaaaccgtg agtggtacat agcagtaaat gatgctgttg gatatggtga
     5161 acctgcaact ttaatatcag gtcttatatt aattccaata atgcttgtaa tagctatggt
     5221 acttcctggt aataaggtat taccagttgt ggatttattg gcaattccat atatggttca
     5281 aggtttagtt gccatacata atggtaatat accaaaagta ttagtttcag gtataatttg
     5341 gtttggatta ggattatatg tttgtacatc tacagcacca ttatttacag atatggctac
     5401 aaatataggt gtagctatac ctgcaggagc aatgttaata acaagtttta atatactagg
     5461 aaaacctttg atgggattag ttttctttgc atttctaagt gcaaatccaa tttatatagg
     5521 tctttcagtt gtaatttact ttgttctatg ggcattgttt agaaagaaca aaacaagtat
     5581 tcttgattat ttagaaaaac aagcacttaa aaatgtagaa gaagagcctg tagcagttta
     5641 agtttataaa aatgaaataa aaataattaa aggagtttat aattgatttt atataagtat
     5701 cagataatac ctgagaaata tttaaatgaa atatagactt ctttttattt gataatattt
     5761 gtaattataa tgcattgtat ctttatagtt gagttttctg tataacctgc tatttaaaac
     5821 atactctttt atatttatac agtaatgtgg tttaatttta taaaatgtaa tctctactgg
     5881 taaaagactt tgtatataag gcatttgcct gaataaacca tttatctttt gtatggcata
     5941 gatattgcta tatagaagta tatagtatga aaggagatat ttatatggga gataaaatga
     6001 gagcatcagt attgtataat gtaggtgatg tcagatatga aatggtagat ataccagaaa
     6061 taacagatac gcaagtgctt gtaaatgtaa aatatgttgg gatatgtggt tcagatttac
     6121 ctaggtctat ggtaagtggt ttgagtggaa atacaaaata tccattgatt ttagggcatg
     6181 aattttctgg tgaagttgtt aaaattggag aaaaagtaaa gcatattaat gtaggagata
     6241 gagtagctgt tgcaccatta gtaccttgtg gaaaatgtga ttattgtaat gaaggtaatt
     6301 ttggcttgtg tgatgattac aatataattg gaacaagagt aaatggagcg tttgcagaat
     6361 atgtaagagt tccagaagaa catattttaa aactaccaga tacattggat tatgaaactg
     6421 ctgccggaat tgaaccagca actatagctt atcatgggat ttcaaagtct aatattagag
     6481 taggagatag tgtagttgtg ttaggatgtg gtccaatagg tcagtttgtt atacaatggg
     6541 caaaggtgtt tggtgcatct aagattatag cagttgatat ttttgatgaa aaattagaac
     6601 tttctaaact tctaggagca aactatatac ttaactcaaa agaagtaaat gtaatcaaag
     6661 aaataaaaaa aataacaaat ggtggtgctg atgttgtaat agaaactgct ggtagccgat
     6721 ttacacaaga acaatctcta tttgtagcaa aaaagagagg aaatatagtg tttgtaggta
     6781 tatcacacac agaattacca cttagtgcag acgctactga gtgtatatta cgtggagaac
     6841 ttaccttgaa aggttcttgg aattcatata caagccctta tcctggaaga gcatggactg
     6901 caacattgga ttttatggga aaaggtgaca ttatttttaa acccatgatt tcacataaaa
     6961 taggtcttaa tgaagtgggt gactttttaa gtaaaatgag caagagagaa attaatttca
     7021 ataaaatcct agtagaaata taaaaattta ataaaaaatt ctaaaagcaa ttaaatttta
     7081 taactatgac attaaataaa ataaaaataa ttttcttaaa taaaatatag gaggaagaaa
     7141 agtgttagaa aatttaaaag aagaagtatt aaaagcaaac ttagaattac ctaagaaaaa
     7201 gctaattact tatacttggg gaaatgtaag tgggatagat agagaaaaag gattagtggt
     7261 gataaaacca agtggtgtag agtatagtaa tatgactgta gaagatatgg ttgtagttga
     7321 tttagaggga aatatagttg atggcaaatt aagaccttct tcagatactc cgactcatct
     7381 agttttatat aaagagtttg aagaattggg gggaatagta catacacatt caagttgggc
     7441 aacaatttgg gctcaaatag gaaaatctat accatcttgt ggtactactc atgcagatta
     7501 tttttatgga agtataccat gtaccagaaa aatgactaga gaagaaattc gcaatgaata
     7561 cgaaaaggaa actggaaatg taatcattga agaatttatg ggaagaaatc caattcattg
     7621 tccaggaata attgtaaatg accacggacc ttttacatgg ggaaaagatg caaatgaggc
     7681 tgtacacaat gcagtagttt tagaagaagt cgcaaagatg gcttattata cagagttaat
     7741 gagtcctgat aatattatgg ataaggttct tatgaataag catttttcaa gaaaacatgg
     7801 aaaaaatgca tactatggtc aaaaatgatt atctggaggt gaattatgct agactcttta
     7861 aaaggtaaag ttgtggaaat agcaattaaa gcagagcatt atggtttatg taaagaaaag
     7921 actggtaatt ttagtattag agataaggat actggatata tacttataac cccatcaggt
     7981 gtaggaagag aagaattaaa agttgaacat atttgtgtat tggatttgga tggaaatgta
     8041 atagaagttg agaaaggtat taagccaagt agtgaggtat tgatgcatat tgaaatatac
     8101 aaaacaagag aaaatataaa tgccatatca catactcatt ctttatatgc aacagcattt
     8161 tcagtagcaa agaaaccaat tataccaatt gtatatgaat cagttaatta tggcggttat
     8221 gtagatattg ctccatacga aagaccagga actttaaaat tagctaagag tgtttcaaaa
     8281 ttactgatag atacagatgc atgcttatta gaaagacatg ggttgacaac tgtaggtaat
     8341 gatttagacg aagcattatt aaaatcaaga tatgtggaag aggtagcaga aatatactat
     8401 aggtctttag tattaaatca atttaaagaa cttgaagtag tagatgttga tgagtttaaa
     8461 tcttggaaat acccagaaga aataaagttt taatctaaat aattttcatt gaagtggtta
     8521 attaaaatat atgtattatt tatatcaatt actatgtttt attatgttaa ttaccgtatt
     8581 ttgcaaatat atttattaca ttttttcaaa aaaatataga atccgaaagc catataaaat
     8641 tgacttggga ttctatactt tttatttatg catttttctt tatctgattt tctggagaat
     8701 ttatatgttc aaaatatttt ttagtttcag atactattat aggtgataat gcaagtaaag
     8761 ctataaggtt aggtattgcc ataagcccat ttacaatatc agcaattatc caaacaactt
     8821 ctaactttaa gaaagcacct aataacacca tagccacaaa tacagttcta tacccattca
     8881 tgccttttac accaaacaga aattcaaaac atctttctcc ataataactc catcccaaaa
     8941 ttgtagtaaa tgcaaataat gttaaactaa ctgtaagtaa tattggtcca aatgtaggaa
     9001 ttgctccgtt aaaagctgct tgtgtcataa cagctccatt taggtctgaa caccacactc
     9061 ctgaaataac aagagagaat ccagtaagag tacagattat aatagtatct ataaaagtac
     9121 ctgtcataga tataagacct tgttccgctg gccattcagt tttagcagca gcagcagcta
     9181 ttggagcact acctagacca gactcatttg aaaatacacc acgagcgata ccatttctaa
     9241 ttgctagtga catagttgct cctaaaaaac caccagtagc agcagtaggt gtaaatgcac
     9301 ttttaattat taacataaaa gtttcaggga ttttattcca aaaagatatt aaaatgattc
     9361 cacagattac aacatatata actgccataa aaggtactat tttagtagct acattagata
     9421 tacttttaag tcctccaaat acaactaaag ctacaaatac tgctaataca acccctgtaa
     9481 ttctagggtc tatcccgaaa ctattattta ttgcatctgt tattgaattt acttgagtaa
     9541 aagtaccgat tcctaaaagt gcaactagta taccacttat agcgaaaaat attgcaagag
     9601 gtctccattt ttctcccata ccatttacta tgtaatacat tggcccacca gacacttgac
     9661 catttttatc tttagttctg tattttattg caagaacacc ttctgaatac ttagtagcca
     9721 ttccaaagaa tgctgctatc cacatccaaa aaagtgcacc aggtccacca gctttgatag
     9781 ctgtagcaac accaacaata ttaccagtac caactgtagc agctagagca gtacataagg
     9841 ctgcaaaact tgaaacatca ccactacctt tgttttcagc agagaatatt aacttaagag
     9901 ctcttggcag tttagttatt tgaacaactc caagcttaac agtaagtagt ataccagtac
     9961 caactaatag taccagtagg ggtggtcccc aaataaaagc atcaactttg tttaaaatat
    10021 caattagatt cataaaaaca ctccctcatt ttttttgctt tacataatca atagaatttt
    10081 taatgaagta attgacagga aataagtata atttattaat aatttctagt gtaataaaaa
    10141 aagctaaatc taaaaaagat tcagctctgg atgtacacta attagcaacc aaaataaaag
    10201 tattagtaaa tactgttttt ggtagataat ccctgtcctt ttgcctgaga gattgagcat
    10261 aaaagccttg ccccttcggc gcccataatg ggtctctcca gagttccgta aattttacag
    10321 tcctactttt aaaagtacct gagagtgtta ctccttcggt gtgctatata gcaactcttc
    10381 tataaaatct taaccggata ttgatttgtc atagcttatt gtattaataa tattaatata
    10441 agtttttatg ttttgtcaac taaaaaatta aaaaacaaag aaaaaatata aaaatgtaag
    10501 cctatttacg aattataaac ttagaaagat gagtaatata cgtgaaaatg aattttgtaa
    10561 tacacataca aatgaagtgt aaaataagtt ttaaataaaa aaataataaa atcttaaata
    10621 attaattaga tatgattaaa ttatggtaat attatagtta cgatattaag aaaagttcta
    10681 tttagttaga ttttaagcgt aatatgagat tatgtataga gaaaggatgt atttttagat
    10741 gggaaaaata ttagtgctag cagaaaaacc ttctgttgga agagatatag caaaagtttt
    10801 aggatcaaaa aatgaaaaaa atggttatat agaggggcca aagtatgtga taacttgggc
    10861 actaggtcat ttggttacac ttgctgaccc cgaaagttat ggagaaagat ataaatcatg
    10921 gagcttagaa gacctgccaa tacttcccaa gcaccttaag actgttgtaa taaaaaagag
    10981 tggaaagcag tttaatacag ttaaatcgca aatgaataga aatgatatag atgaaatagt
    11041 tatagcaaca gatgcaggtc gtgaaggaga acttgtagca aggtggataa tagaaaaatc
    11101 acaggtaaaa aaacctataa aacgtctttg gatttcttca agtacagaca aagcaataaa
    11161 agacggattt gcaaaattaa aaagtggaaa agaatatgaa aatttgtatt actcagcaat
    11221 agcacgagca gaagctgatt ggattattgg aataaatgca accagagctt tgacaacaaa
    11281 gtacaatgca cagctttctt gtggaagagt acaaactcct actcttgcaa tgttgcttaa
    11341 aagagaagaa gaaataagaa atttcaaacc taaagagtat tatggattag aattaattgc
    11401 tacaaaggga aattcagata taaaatttat ttggaatgat aaaaacaata attcttcaac
    11461 ttttagtaaa gaaaagatag aatccacatt aaaaaaagta aaaggtgtag acataaaaat
    11521 atctgaagta aacaagtcat ataagaaaaa atattcacct gcactctatg atttaacaga
    11581 attacaaaga gatgcaaata aaatgtttgg attttcagca aaagaaactt tatctataat
    11641 gcaaaaactg tatgaacatc ataaagtact tacttatcct agaactgatt ctagatattt
    11701 aacatcagat attgttggaa cattaaaaga tagaataaaa gctgtaaata ttagtgatta
    11761 ttcaaagatt tgtaatagat tattaaaaag caatattata gctaataaat cgtttgttga
    11821 caattcaaag gtaacagacc accatgcaat aataccaaca gaacagaagg tattcatgag
    11881 tgattttagt gataaagagc gaaaaatata taatcttgta gtaaaaagat ttttagcagt
    11941 actatctcct ccttttgaat atgagcagac aactataagt gcaactgtag aaggagaaat
    12001 atttaaggct aagggaaaca aagtaataaa tttaggttgg aaagaaagtt ataaagatat
    12061 tgaagatgaa gatgagtatg aaagtatacc aaatgtttta gaaaaagatg tacttaaggt
    12121 caagggatta aagataactt caggaaaaac aaatccacca ccatacttaa atgagggaac
    12181 attattgact gctatggaaa agaatggtct agggacagtt gctacaagag cagatataat
    12241 agaaaaatta ttcaattcat ttttaatgga aaagaaaaat aaagatatat atataacatc
    12301 aaaaggaaga caattattag atttagttcc agtagactta aagacaccag aacttacagc
    12361 ttcatgggaa aagaaattat tggatatttc aaagggaaaa cttagtaaaa atacattcat
    12421 aaatgacatg aaagattatt caaaagatat tgtactagaa gtaaaaaata gtgataataa
    12481 atttaaacat gataacttaa ctaaaaatag atgtccagaa tgtggaaaat ttatgttaga
    12541 ggtaaatggt aaaaaaggta agatgttgat ttgtgaagat agagaatgta atactagaaa
    12601 aactatatca caagtaacca attcaagatg tcctaactgt cataaaaaat tagaacttcg
    12661 tggagaaggt gaggggagaa tcttcacttg tagttgcgga tacagagaaa agttgtcttc
    12721 tttcaacaag agaaagcaag aagaaaaaca gaaggcatca aaaaaagatg ttaatcaata
    12781 tttaaaaaat caaaataaaa atcaagagac atttaataat ccatttgcag aagcattagc
    12841 taagctaaaa aacaaataat tttaaagtta ttattataca caattataac tttaactgta
    12901 tattagttat atgtactgat ataatatagt gtatagttta ggcatgaaag tagggctgtt
    12961 aaatggattt aagtggcata tttaaatact attgtaaaga gtgtgaaaat acatggaata
    13021 attcgagtgt tgaattattt gagaatatag aaacgtatag taaagattca caaaaaaaga
    13081 gggaaaaaga attagataaa ttgctaaata caatatcagt tcatttagag aggtatccaa
    13141 gtgatgctgt attgagaaaa atgtgggtaa aaaagggcga ggttttctta caaaagacat
    13201 tggaaaaaga aaatattttt aagttagaaa aaatggatgt agaggataga aaaaaatttt
    13261 tagatataac aaaacagttt attagagatg ctagaaaatt tgatgatgat ttacctatag
    13321 gtgatattat gcaagctatg aggaatgtat ggatttcaaa tgcattacaa ttattatttg
    13381 gtaaagaagt atattattca aaagctaact ttgcatatag tatgttatat ccatatactg
    13441 ataattattt agacaataca aatatagata aaaatgataa gattttattt aataactggt
    13501 tagaaaaaag gctcctggga gaacacatta aatctaagga ttatcatgaa agtaaagtat
    13561 ctcaaatgat agattatatt gaaagtgtat accctagaga aaagtttaca gaagtttatg
    13621 aatcgttatt attaatattt aaaagtcaag taaatagttt aaaacaacat ggtaaggaaa
    13681 atcatttgtg taaagaagat ttattatcca tttctattga aaaaggaggt tcatccgttt
    13741 tagtagatgg atatttaata agtggattga tgacaaagga agaaatagag ttttgtatag
    13801 gatatggatt tttattacaa atatctgatg atttacaaga tataaaagaa gatttaaaat
    13861 acaaccataa aactattatt acagagatgt caaaagaggg tactttagat aaagttgtaa
    13921 ataaactaat aaattttact attgagttaa tagatagttt taaaattaat aataaaaata
    13981 aatctgtaat aactatgata aagaatgatt gcttaatgtt aattttattt tctgtagttt
    14041 ataatgctga atttttttct gtaggatata taaaagaagt agagaaattt attccatata
    14101 caatagatta ttcattagag attgaagaaa aaataaaaga aaaatttaaa aatatagatg
    14161 ttttaaataa tgaaaatgaa tataaagaaa tgattgatat tatttgtgca gagtagaaaa
    14221 aaagggagtg tcttagaata gactttttag tttatgaggc actctttttg tataaaatca
    14281 aatgtatttt catcatttta acaattaaaa agaacttatc cctatttgag aaagcccttt
    14341 ttaatttaaa taaaaatgtc aaggttaaac tatttaaatg cagtaataat cccattatca
    14401 tgtaagtttt ttatgattgt agctgcgata gggaatccaa ataatccagc tattccaaat
    14461 agttgagccc caacaaacat acacattaat gttattaaag gatataggcc aatttggtgt
    14521 cctactacct ttggttctaa tatttgtcta acaacggtta taattatgta tagtattaaa
    14581 agtcctattg ctaaattaat atttccatta atgagtgaga taatcatcca aggtgtcaaa
    14641 atacttcctg tacccaagac tggcaaaata tcaacaagtg ctatgatgac agatatagta
    14701 aatgcatttt caacctttag gatagtaagt ccaataagta gttctatgaa tgtcactgat
    14761 agtagtattg cataggcttt tagtagtttt aaaagagcat taatactgct attttttata
    14821 tttattatca taaattgact tttttctgga aattgtttta agataaagtt tactattttt
    14881 tgatagtcaa aagtaaagaa aaaagaagca attaaagtaa taataagttt taacataaat
    14941 gcaggtgttt tacttgcaat actagcaata gcattgagtg catttgttga aatagaagct
    15001 actaaagaca tcatagattg acttatatta tcccaaccaa gtattaatgc taggttaaca
    15061 ttaggtgtac tagcatctat ctttgaaaat aaagtattta gcgttggttg tatacttgat
    15121 ttatatattt caggaagtct aaaaaataca tcacttacac ttgcaaatat cttcacacca
    15181 aagctaacta ataaaaatat acacaaccca taaaaaatta aaagtattac tatagaaata
    15241 agtgtacgat ttaaatttgt attatttgta ataagtctta taataggtct taagatggat
    15301 gcaatgataa aactgattac aaaaggcatt aataatggca ataagaattt taatacaata
    15361 tatacaatac ttgttattaa aactacatat gtaaatttaa ttataaaatt cttttgcttt
    15421 tgaatatcca taatataaaa cctccaatgt atttgatatt attctaaata tggtcttttt
    15481 tacagaaaat atactactgt atattgatta taaactataa agcttatgta aagtaaataa
    15541 attatgtaaa atttaggtta attttgtata aagagatact tttatatttt acaattaata
    15601 accaaatact aaaaaatatt ttaaataaag aacttaaaat tgtgataaaa ttaatttata
    15661 gatattaaaa aaagtataat atatggtttt taagaggtgc taagatgaaa aattttagag
    15721 atttaggtgg gaacaaaact gaggatggca gaactgtaaa gaagggatta ttttataggt
    15781 ctgcaaaatt atcaaatcta agtgaaaatg atataaaaat tttaagagag ttaaatatta
    15841 aatatatatt tgattataga agtgatgaag aagctcgtaa acatccatca acaataatat
    15901 caaatataaa aaatataaga ataccagcaa tgagagagct agaagaatca ggaggaagtt
    15961 ttggttctat tgaagatatg attgatggat tgtttgaaaa agatggtgca tttaatatgt
    16021 taaataatag ttactataat ttgcctataa ataatccgtc atataaaaaa ttagtagaac
    16081 ttataagaga ctattctaat ttaccaatat taaatcattg tacagctggt aaggatagaa
    16141 ctggtgtggg gagtgctata attcttatga ttttaggggt atcgagagag aatataatga
    16201 aagattatct aaaaagtaat gattttgcag ataaagagat agaaagattt atagaatata
    16261 agcctaaatt taaggacatt ccaaaagaaa atttgaaata tatatttggt gtaaatgaag
    16321 agtatatgaa aactgcattt agaagaattg atgaagaata cattagtgta gaagcatatt
    16381 tatatggaga atttaattta aataaagaag aaataagaaa attaagaaat caatatctag
    16441 agtaaaaggt gagtttatga gtttaaatat aacagcaaga cagataaata taataaaaat
    16501 attgttaaat tcaaaagata caattagtgg tattgcctta agtcaagaga taggatgttc
    16561 ttcaaaaact atacaaaatg aaataaagga tattaataaa caacttagaa atggtaaaat
    16621 tttgtcaata agaggagttg gttataaact agaaggaagt tttgatgagg taaatctaaa
    16681 tagcaatctc tatgatgatg tagatagagt agaatatata atcagaaaaa tattaacctt
    16741 aagtaatgaa gaaaaaaata ctataaaact tgaagattta gccgattcta tgtatgtaag
    16801 tgtttcaaca gtaaaaaatg atttaaaaga agtaaaaaga atgttggaaa agtattcaat
    16861 atcagttaaa tctaagcata aacaaggtat atgtgttttg gaaagtgaag ataagatatt
    16921 aaattgtata gttgacttaa gcaataagag agataatcaa ttgagtttaa atgatttttt
    16981 aaatgatgat ataaaggaaa gaaaactatt aataaaaaaa ttattattag acatattaaa
    17041 tgaaaatgga ttagtattaa ctgatataga atttaagagt gtgttaaata atatattagt
    17101 tatgttaagc agacataagt ataatgaaaa agaatttata gagaattata tagaaaatta
    17161 taggagcaaa agaagctcaa ttttagaaaa tgaacaaaac aaagaaatta taataaattc
    17221 aataaaaaca ttttgtaaaa atttaaaatt agctacatca atagatataa gtaatgataa
    17281 ggtatttgaa gagtttttat ataagcatat tagtagttta tgtaaaaaaa taaagttagg
    17341 tataaatcaa tcacctataa tggctcaaga tataaaaata aaatatccat ttgcttttga
    17401 actagctaat atagctaaaa agacaataga aaaagatttg aagatagaaa ttaatgaaga
    17461 tgaagttgca aacatagctc tacatatagg aggagcagtt gaaagagcat cttataataa
    17521 taaagataag gttttcaaaa ctataatagt ttgtacttca ggcataggca catcaatgtt
    17581 aataaaagct aaattagaga atatatttaa ggaaaaatta gaaatactaa agattattcc
    17641 ttcatactta gttgaatata taaaagttat agatgttgat tttgtaatat ctactgttcc
    17701 tattgaagta ggagatgtgc cagtaataaa tatatctcca attttaagtg agaaagaaat
    17761 tagattaatt gaaaaatata tagaaacagg aaatatatac ctagatttag atataaaaag
    17821 cctgtttgat agtgaattgt tttttactga tttggtgttt aaaaggaaag aagaagttat
    17881 agattttatg gcaagtaaac ttgtacaaaa gggctatata gatgaggata tgaaaaaatc
    17941 ttactttgag agagaaaaaa tagcaacaac agaaataggg aatatggtaa ctataccaca
    18001 tggagctatt ggaaaaattt ttgataataa aatagcaatt ggtatattaa agaagcctat
    18061 ttcttgggaa atatcagatg ttcgtttaat aataatgtta gcccttgata aagataaaat
    18121 attagattat gaagtaatat tttcaaaaat atataaaaga gtagattcta tagcaaaagt
    18181 tataagtata tgtgaaaata gtagttttga aaaatttgta aatttattta attagatggg
    18241 tagtattgta tatacacctg taaatttgga taatataaaa ctaaataaga taaaaacgat
    18301 aaatttctct tttaattaag agaaattttt tgactttcaa ggatattaat ataaagttaa
    18361 gatataaaca taaagcaaat aaataaaaaa gaggtggcaa tatgatttat acagtgacat
    18421 taaatccatc aatagattat atagttaagc tagatgagct aaagacagga agtacaaatc
    18481 gtgtaaaaga agaatacgtt tatccaggag gaaaaggtat aaatgtatct cgcatactta
    18541 aggaacttgg aaatgataat acatctttag gttttataag tggttttact ggagaatata
    18601 taataagaac attggaagaa aaagaattga agacagattt tataaagata aaaagtggtt
    18661 tttcaagaat aaatgtaaaa ataaaggcgt taaaggaaac tgagattaat ggacaaggtc
    18721 caaatataga tgatgaagac attgatactt tatataaaaa acttgataag ctaaatcaag
    18781 atgatatttt aatattggca ggaagtatac catctacttt agatgaaaaa ctatacgaaa
    18841 atataatggc tagattagaa aaaaagaata taaaagttgt agtagatgca actaaaaatc
    18901 ttcttcttaa tgttttaaaa tataaacctt ttctaattaa gcctaataac gacgaattag
    18961 aagaactatt tggtgttaaa ttaaattcaa ttgaagacat ggtaaaatat gctagaaggt
    19021 taaaggaaat gggagctata aatgtcttgg tatcaatggg aaaagatgga gcattgttga
    19081 ttacagagga agatgaagtg cttataagtg atgttccaaa aggaaaagta aaaaattctg
    19141 taggagcagg tgattcaatg gtagcaggat ttatatctgg gtatttgaat actgggaaat
    19201 atgattatgc tcttaaattg ggagcagctt caggtagtgc aactgctttt tcatatgact
    19261 tagccaaaag agaatacatt gataaattgg taaatgagat atctgtaaag cagttttaaa
    19321 atattaaaaa atatatagaa aggtaattaa tatttatgaa aattatagat ttattagatg
    19381 aaaagtcaat taagttaaat ttaaattcaa aaaccaaatc agaagcaata gaagaattag
    19441 tagatttggt cgcaaatagt ggaaatttaa atgataaaga aaactacaaa aaagcaattt
    19501 tggctcgtga agaaatgagt acaacaggaa ttggagaagg tgtagctata cctcatgcta
    19561 aaaattcttc agttactaaa gcttgtatag cagcagcagt gtcaaaagaa ggtatagatt
    19621 atgaatcgtt tgacggaagt ttatcaaatc tattttttat gatagctgca ccagacggag
    19681 ctaacaatac tcatcttgaa gtattatcaa gattatctac aatacttatg gatgaagact
    19741 ttagaaataa acttataaat tcaagctctg aaaaagaatt taaggaaata atagataaaa
    19801 aggaaagaga aaaattcagt gaagaatatc aagatgagaa agttactgaa aaaaatataa
    19861 tagatactaa agagaatgat gctaataagt atcctaaggt tttagcagta acagcatgtc
    19921 caactggaat agcacataca tttatggcag ctgaaagttt aaataaaatg gcagaaaaca
    19981 aaggtgtatc aataaaagta gaaactaatg gttcagcagg tgttaaaaat aagcttacaa
    20041 aagatgaaat agaaaatgca acatgtataa tagtggcggc agataaaaat gttgagatgg
    20101 ctagatttaa tggtaaaaaa gtcataaaaa ctaaggtggc tgatggtata cacaaagcgg
    20161 aagaacttat agacaaggct gtaaatggag atgctccaat ttaccatgga ggagatggaa
    20221 gtcacaacga atcaaatgaa gaaagtgaaa gtggatttag aaaagtatac aaacacttaa
    20281 tgaatggtgt atctaatatg ttaccatttg ttattggtgg agggatactt atagcgatag
    20341 cctttttact tgatgattat actattaacc cttcaaactt tggttcaaat acacctatag
    20401 cagcattttt taaaggaata ggagataaag cttttggatt tatgttacct gtacttgctg
    20461 gatatatagc atactctata tctgatagac cagcgtttgt tgtaggattt gtaggaggtg
    20521 ctctagcagg tgatggtggt tcaggttttt taggtgcact acttgcaggt tttatagcag
    20581 gttatttagt agaagggctt aagaaaatat ttagtgtact accagcttca ttggaaggaa
    20641 taaaaccagt attgttatac cctttacttg gaacactttt gatgggaatt ataatgacat
    20701 ttttagtaat acctccagtt acagcaatta ataatgcaat ggttggattt ttaaatggtt
    20761 taggtggaac ttcaaaaata gttttaggat tagtattagg tggaatgatg gctgtggata
    20821 tgggtggacc tgtaaataaa gcagcatatg tatttggagt tgcatcactt gaatcagggc
    20881 agtttgaaat aatggcagca gtaatggctg gtggtatggt tccgccactt gctatagctc
    20941 ttgcaactac attttttaag aatagattta ctaaagaaga aagagattct ggtaaagtta
    21001 attatgtaat gggattatca tttgttactg aaggagctat accttttgca gctggagacc
    21061 cacttcatgt tataccagct tgtgttggag gttcagcagt ggcaggagca cttagtatgt
    21121 tgttcaacgc agctcttcgt gcacctcatg gaggagtatt tgttatacca gtggttaccc
    21181 atccatttgc atatatatta gctatagctg taggtgcttt ggttggaatg atgcttttag
    21241 cattacttaa aaaaccatta aatcaagaag tatagcaatt aaaggatatt caagaactta
    21301 aaatatgatg ttttataaaa attaatatat tgctgaatta ttttaaaata aggattttat
    21361 gaggctgtta tataacgatt tgtaattgtt atcttgacag tcttttattt ttatataagt
    21421 aaaataagtg tgttttgctt tatttgtatt taaattgttt ttaagttaga atataaagaa
    21481 aattatatat tattttaata ttcactcgaa ctagaaaata atatttgttg tatttttttg
    21541 tatataaaat atattgtaat attaaaaaaa taatagtaaa ctgattgcat aaaaatgatt
    21601 tttatataaa atttaagcag aagaataaat ttctaacgaa attggatatt tcttctgtaa
    21661 atacatattc aaaaaaagaa ttataagagt ttagtcagca gtcgtgtaag taataaggac
    21721 tttataaata tatagaaata taatgatatt aatataacca agcgattttt aatttagctt
    21781 tttaataagc ttatttttta tactatttat aataaataaa ctccatgatg ggaaaactat
    21841 tactatttaa tatgtaaagg agtattttaa atgaatgaat catattggtt tttaaactca
    21901 agtccaaaag aatataacaa attaggtgaa aatataaaaa cagattgtct tatagtaggt
    21961 ggtggaataa cgggattgac aactgcatat ttattagcaa aagaaggtaa aaaagtagtg
    22021 cttgttgagg ctgataaaat aggatatgga acttctggaa ggaatactgg taaggtaact
    22081 tgtcagcatg atatatttta ctctaagata gagaaaaaat atggattaga taaagcaaaa
    22141 tcatattata atgcaaataa tgaagcttta aatttagttg aacaaataat agaagaaaac
    22201 aatataaaat gtgattttaa aagggaaact tcttttgtat ttacagaaaa agaggatact
    22261 ataaaggata ttaaagatga atatcgtaca tgtaaaaaaa taggaataaa ttgtgagtat
    22321 catgaaacta tagaaaatat tccattagat ataaaggggg ctattagttt tactaatcaa
    22381 ggtcaattta acccaaagaa gtatattgat ggattggcaa aagcagcagt aaatttaggg
    22441 ctaaaaatat atgaaaatac accagtagta gaccttgaaa aaggaaaaat atgtagggtt
    22501 aaaacaagag aggataatat aatagaagct gagaatgtta taatttcatc tcatagtcca
    22561 tggtatgatg gattgaattt ttattttgca aaagaatatg cagagcgtgc ttatttaatg
    22621 gttgcagttt tggaaaataa attagcagat ggtatgttta taagtataga tgacccatca
    22681 ataactttta gacaatataa tgatggaagt gaaaatttac tgatttttgg aggaggagac
    22741 cataaagttg gtcagggagg aactgagaaa gaaatatttg atgatttaga gcattatggt
    22801 aaagaagtct ttaaggtaaa agattttaag gggaaatggt ctgctcaaga taatatgagt
    22861 tttgacaatg ttccttatat tggttatatc aacaaaagag aagacaatat ttatgtagct
    22921 acagggttct ctaaatgggg aattactaat ggaactgctg ctggaataat aataaaagat
    22981 ttaataataa ataataattc tgattataaa gatactttta atccatccag actaggtagc
    23041 tacttttcaa aagattttat aaaggaaaat gcaaacgtag caataaatta tgtaagtgga
    23101 aaattaaaaa taggtagtgg agatatgcct aaaaataatg gagaaggaaa gattgttaat
    23161 attgatggaa aaagatatgg tgtatataag gatgataatg gagattttta tattgtagac
    23221 actacatgta ctcatttagg atgtgaactt aattttaaca gtgaagaaaa aacatgggac
    23281 tgtccatgtc atggttctag atttgactat aagggaaata tacttgaagg accagctctt
    23341 aagccattaa aactttatgg acatggtgat aatgatgtta atcctaagtt attataaaat
    23401 tatttttagg aaggtaaagt gtatatgaat aaacttttaa agccaataac tatactattg
    23461 tatatagttt atattttatc tgccgtattt tttgtagcaa acaaagaata tggaagtcta
    23521 ggtctagtag caattagttt agttggtact ttcattttga gttttataaa taagcaaact
    23581 gaaagattat tgagtaaaga attatatata atgcttgctt tatttataat gttttcatca
    23641 ttgttaggta cttgttttaa tttttatgat ataaattatt atgatgattt tttgcattta
    23701 tggtcaggta taatatcttg tattgtggct ttttctataa ttaaatactt ttatacacaa
    23761 agagagataa gtaaaatgag taaagtgttt ttagttatat ttttatttat gtttagtatg
    23821 ggagtagcga gtttatggga aataggtgaa tttttaatgg atacttttgt tgggactact
    23881 acacaggccg gagggttaga agatactatg attgatatgg tagatgcttt aataggcaca
    23941 attataacta ttccttttat agtaaaaaaa tctaatatat agttttaaat tttttatatt
    24001 aattataatg g
//
LOCUS       DAEOSP010000040        23570 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.040, whole genome shotgun sequence.
ACCESSION   DAEOSP010000040 DAEOSP010000000
VERSION     DAEOSP010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23570
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.040"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..283)
                     /locus_tag="KRH54_001310"
     CDS             complement(<1..283)
                     /locus_tag="KRH54_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBG8578977.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFG"
     gene            complement(293..1222)
                     /locus_tag="KRH54_001311"
     CDS             complement(293..1222)
                     /locus_tag="KRH54_001311"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBG8578978.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(1237..1677)
                     /gene="def"
                     /locus_tag="KRH54_001312"
     CDS             complement(1237..1677)
                     /gene="def"
                     /locus_tag="KRH54_001312"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG8578979.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(1695..4220)
                     /gene="priA"
                     /locus_tag="KRH54_001313"
     CDS             complement(1695..4220)
                     /gene="priA"
                     /locus_tag="KRH54_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBG8578980.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(4459..5658)
                     /gene="coaBC"
                     /locus_tag="KRH54_001314"
     CDS             complement(4459..5658)
                     /gene="coaBC"
                     /locus_tag="KRH54_001314"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBG8578981.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(5663..5929)
                     /locus_tag="KRH54_001315"
     CDS             complement(5663..5929)
                     /locus_tag="KRH54_001315"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBG8578982.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(5932..6549)
                     /gene="gmk"
                     /locus_tag="KRH54_001316"
     CDS             complement(5932..6549)
                     /gene="gmk"
                     /locus_tag="KRH54_001316"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBG8578983.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLKELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(6579..7460)
                     /locus_tag="KRH54_001317"
     CDS             complement(6579..7460)
                     /locus_tag="KRH54_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBG8578984.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(7464..8294)
                     /locus_tag="KRH54_001318"
     CDS             complement(7464..8294)
                     /locus_tag="KRH54_001318"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBG8578985.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(8581..9615)
                     /gene="ltaE"
                     /locus_tag="KRH54_001319"
     CDS             complement(8581..9615)
                     /gene="ltaE"
                     /locus_tag="KRH54_001319"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBG8578986.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            9911..11686
                     /locus_tag="KRH54_001320"
     CDS             9911..11686
                     /locus_tag="KRH54_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBG8578987.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(11901..13553)
                     /gene="abc-f"
                     /locus_tag="KRH54_001321"
     CDS             complement(11901..13553)
                     /gene="abc-f"
                     /locus_tag="KRH54_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HBG8578988.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(13852..15063)
                     /locus_tag="KRH54_001322"
     CDS             complement(13852..15063)
                     /locus_tag="KRH54_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBG8578989.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKIVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIASITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISIGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(15347..15880)
                     /gene="pyrR"
                     /locus_tag="KRH54_001323"
     CDS             complement(15347..15880)
                     /gene="pyrR"
                     /locus_tag="KRH54_001323"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBG8578990.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(16240..17187)
                     /locus_tag="KRH54_001324"
     CDS             complement(16240..17187)
                     /locus_tag="KRH54_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG8578991.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(17156..17602)
                     /gene="lspA"
                     /locus_tag="KRH54_001325"
     CDS             complement(17156..17602)
                     /gene="lspA"
                     /locus_tag="KRH54_001325"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBG8578992.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(17912..18691)
                     /locus_tag="KRH54_001326"
     CDS             complement(17912..18691)
                     /locus_tag="KRH54_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG8578993.1"
                     /translation="MKKIIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(18746..19228)
                     /locus_tag="KRH54_001327"
     CDS             complement(18746..19228)
                     /locus_tag="KRH54_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG8578994.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(19494..20915)
                     /locus_tag="KRH54_001328"
     CDS             complement(19494..20915)
                     /locus_tag="KRH54_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG8578995.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(21061..21831)
                     /locus_tag="KRH54_001329"
     CDS             complement(21061..21831)
                     /locus_tag="KRH54_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8578996.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELFNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(21836..23038)
                     /locus_tag="KRH54_001330"
     CDS             complement(21836..23038)
                     /locus_tag="KRH54_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG8578997.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT         7646 a         4186 c         2354 g         9384 t
ORIGIN      
        1 taccaaatga tgatgcaatg aaagatataa taggaaataa tccactgaca atattaaaat
       61 ttattttcat tatatttgag ttaacatact ttcctttaat tactctataa gtaactgctg
      121 ttgaacatag tattataagt attagtgata ttattataat atttatcatc aaggagaata
      181 tttgagttaa acttattata aaaaaattaa caactcctat caaaattcct aatattaaaa
      241 tcaaaatccc aacacttgtt aaatacttct ttatatctgt catacttttc acctaaccca
      301 atactaaacc agtttctata gtattccctt ttatatattc accaaccaac attctctttt
      361 tattcggcat ctgaatttct tcaattaaaa gtacattatc tatagtactt acttttatcc
      421 catctttgct tacatcaatt attgtaccta catcttttgt gcttttttca tctaacactt
      481 tagttttcca aactttcatt atgacatcat tataggtagt ataggcactt ggccatggat
      541 ttactcctct aactaaattg tgtatttctc tggcactttt tgaaaaatct atgttaccta
      601 atgatttatt cattattggc gcataggaaa attcttcatg attttgtact tctcttggag
      661 catttccttc ttcaattaat cttaatgttt cttttaaagt ttctgcgcct atattcatca
      721 ttttatcatg aagttctcct gctgttatat tttcatcaag atttacttct gtctttagta
      781 tcatatctcc agtatctagc ccttcatcca tatacatagt tgtaacacca gtcttttctt
      841 caccatttat tattacccaa tttataggtg ccgcacctct atattttgga agtaaagaaa
      901 catgaacatt tatacatcca agtttaggaa tctctaatat ttcttttgga agtatctgtc
      961 caaaagctac aactactatt acatctggat ttaaagattt aattttatct ataaattctt
     1021 tatctctagc ctttactggt tgatatacag gtatattatt ttcaattgcc agttctttta
     1081 ctggactcat acctagtttt ttacctcttc cttttggttt atctggttga gttacaacac
     1141 ccaatatttc atacttctca tctattattt tttgtaaaca tggaactgct atatcaggag
     1201 ttcccataaa tactattttc atcaaatcaa ctcctactac ttttctattt tgtcaacaaa
     1261 caaaattcca tctaagtggt ctatctcatg acaaaatgct ctagctaaaa gttcttctcc
     1321 ctctaactct attgtttcac catttctatt taaagctctg acctttacat aatttggtct
     1381 tctaacattt cccgcttctc caacaacact taaacatcct tcctcatcta tttgctcccc
     1441 agatgtttct attatttcag gatttataag ttctataagc tcttctccaa tatctattac
     1501 tactactctt tttaatattc caacttgagg tgcagccagt ccaacaccat ctgcatcata
     1561 catagtttct gccatatcat ctaataattg tattatcttt tcatcaattt tttcaacctt
     1621 ttttgatttt tttcttaata ctggttctcc tatttgaact atttgtctta aagccattat
     1681 aattcctcct aaatttataa tacactgttt ggattaatat ctatagatac attgatattc
     1741 ttatcaaata tcaaatctcg ttttgttata catatatatt ttattatacc ctttaagaga
     1801 ttaatttcaa tattttcatc tttaaagagt atttgccatc tataattttg atttattttt
     1861 gatattgaac atggatttgg acctaatata aatcctaaat catttatacc ccttttttct
     1921 aataaataaa tcaatgatgc atgcatattt tttatatttt ttatcactag tctttcatct
     1981 tcaccactaa caactacact taacatatta ttaaaaggag aatatccaaa tgcttttctt
     2041 atttttatct catcttcata aaaaccctca taatcatatt caatagcatg cttaatagca
     2101 tagtgttcag tgtcataagt ctgaagtact acttttccct ctttatcagc tctccctgct
     2161 cttccagaga cttgagttat caattgaaat gtagtttcaa aacttttaaa gtctggaaaa
     2221 tttaatatca tgtctgcaga caaaatacct actaaagtta cattctcaaa atccagccct
     2281 ttacttaaca tctgtgttcc aataagtaca tctgcttctt tatctttaaa tttatttagt
     2341 atctcatcta aagcaccttt ttttgaagtt gtatctttat ccattctcaa agtttttata
     2401 tctggaaaaa tatattttaa ttcctcttca attttttgag ttccaactcc aaatggctta
     2461 acataagtac ttttacattc aggacactct tttggaattt ctttctcata tccacaatag
     2521 tggcatcttc cagtattgct tttcttatga taagttaaag atatatcaca attttcacat
     2581 tgaaacacat atccacactt tctacaagat acaaaatttg cataacctct tctgtttaaa
     2641 aatagtatta cttgattatt ttcttcaatc gcataacgta tttctttttg taatttcaaa
     2701 ctaaatatac ttctatttcc tttatctaac tcatctttca tgtctaccac ttcaatgttt
     2761 ggaagaggct tatcatttgc tctacttttt atatttataa gtttgtattc tccagatttt
     2821 gccctataat actcttcaat tgagggcgtt gctgaaccta aaataagagt tatattattt
     2881 tttaatgcca taaatctagc aacttctata gcattaaatt ttggattctt ctcagactta
     2941 tatgatgact catgacattc atcaattatt attaagccta aggaattaaa aggtgcaaat
     3001 aaagctgacc tagctccaat taacactctt actttacctg ctttgacagc tttgtaaaca
     3061 tcatgttttt ctccttcaga tagtttactg tggaacactc caaccaaatc tccaaaccta
     3121 ctctttagcc tatctattgt ttgaggagtt aatgctatct caggtactaa gaatatactg
     3181 tctaagcctt gatttagagc aaattctatt atttccatgt aaacttcagt ttttccactt
     3241 ccagttactc catgaattaa gtatggtttt ttatcatcta caaacatatt cgactttatc
     3301 tcatcaactg cttcttgttg ctcattgttt aattttatac tttttagtac ggatttgtaa
     3361 atactttttg gctctctata ataatcctta aattctaggg ttattagctt tttcttactt
     3421 agagaattta tactttgttt tgaagcattt aataaatcca ataaatcatt tatctctaca
     3481 ttttcattat ttttaagaaa acttaatatc tctttttgtt tacttcccac atttatttta
     3541 ttttgctcta tataatcgtc aatcttatca ctttctaaac tcaaagatat ataacatacc
     3601 ttcttttcat ttttgtgatt tttatattcc caacataatt ttataattcc attcttattc
     3661 attgtgtaca aagaactatt taagtttggt atatatttta acttatcaac cttaatttta
     3721 cctttactat caatcacttt ctctactatt tctctatttt tatcacttaa agttgatatt
     3781 ttttccttca attcaatatc atttaatcca cttaaacttt cacctaatag gactacttta
     3841 taattattta atttgtatcc ctttggatat atcaaattta tacattctat ataagtacac
     3901 aaatatctat ttttcatcca ataaaccaac tctaaatctt cttttctaaa tatcggattt
     3961 tcatctaata tatcaataat ttcttttatt tttatttttt catctaagct atctgtaaat
     4021 tgaaatacaa aggcttctgt tggtttatta ccttttccaa atggtactaa tattctgtgc
     4081 ccaattttta ttacatcaga taaaaataca ggaatttgat atgtaaataa attatctgta
     4141 tatatagtat tacttctaac tattaccttt gcatattttt tcattataaa acctctactt
     4201 aaatttaata tattgttcat attttaatat actttacaag atattaaaat ctcagaattt
     4261 ataagcaatt tcaagttatt aatattaact ttattttaat aattaactat taattatatt
     4321 ttatacttta tgacaaaata aatgctgaaa actacaattt aactgacatt tgcgagtttt
     4381 tactcgctaa aattaaattg cagctttcag ccactttagc tgtgataaaa tacacaaata
     4441 tcatatttaa aaaaggtctt atttctcaag caacgcttta actttatcta aaataacatt
     4501 ggctacttct tcttttttca tcttaggata ctcagtaata tttccctcta catctattat
     4561 ttttacaata ttagtatcag ctctaaaacc agcaccttct ttagttaaat catttgccac
     4621 aataaaatct aaatttttct tttttatttt tcctttggca tgttctataa ggtcattagt
     4681 ttcagctgca aatccaatta gtatcttatc ttcttttatt tttcctattt cataagcaat
     4741 atcataattt ctacttagtt ctattgataa atcatcattc ttctttttaa tttttttgtc
     4801 agaataagtt tttggtttat aatctgcaac tgctgcactt tgaattacta ttttattttc
     4861 acttaaattt gatactactg cttcatacaa atcttgtgct gactctattt gaacaaattt
     4921 tttaagattt tgtggaatag aaagatttgt aggacctgat attaatgtta catcagcacc
     4981 tctagctata gcttctcttg ctatagaata tcccatttta ccagaagacc tattagttaa
     5041 atatcttact gggtctatac tctctacagt tggaccagct gtaactatca tactttgacc
     5101 ttttaaatct tgctctttag taagattagt caaaacttcc tctactatta tatctggatt
     5161 agctagttta cctgttccaa tatctccaca tgcaagcctt ccactttccg gttcaataaa
     5221 tttatatcca aagctcttta aagtttgtat atttttttga acaattggat tttcatacat
     5281 attagtattc atagctggtg ctataagaac ctgtgctttt gttgccataa cagtagttgt
     5341 caacatatcg tcagctatac catttgcaat cttgcctata acatttgcac ttgctggcac
     5401 tattaagaat aaatctgctt tttttgctaa tgatatatgt tcaacttccc aagtttttgg
     5461 ttcttcaaac atatcttcta ctacataatt ttgactcagc gattgaaatg taagtggttt
     5521 aacaaattct gttgctgatt ttgtcattat aacatggata tttgcattta gtttttttaa
     5581 cttacttaca acatcacaag ccttatatgc agctataccg ccacttatgc caattacaac
     5641 agttttatct tttagcatga ccttactcct cttctgaaat ttgttggtgt tgctcagcat
     5701 gatgtctagc ttcttctatc tctatttcct cttctgtcaa taatctatat gttatttttc
     5761 cacttgcaac ttctttagtt gctacacata caggcttttc cttagtttta tttgaaacat
     5821 atggttcttc tccatcaatt aattttcttg ctctctttga aacagtccct actaaatagt
     5881 atctgttgtc tattttttct agtacctcat tgatagatgg ttttaacata attataattc
     5941 ctccttaaat ttctctatta taatattttt atatctgcta actttattct tttcagatga
     6001 tattatacct tctatttctt tagcagatgt ttttacatct tcattaaata taaaataatc
     6061 ataatcctta atcattttaa tttcttcata tgcacagcta aatctcttct ctatttcagc
     6121 ttctgtttct gtacctcttc caacaattct attttttaat tctttaagtg atggtggtag
     6181 tacaaatata aatactccct ctgggcaaac ttcttttatt tgttttgctc cttgcatttc
     6241 aatctccagt aatacatctt gtcctttttc taaacattcc attattgccg cttttggagt
     6301 tccataaaaa ttatcataaa tttgagcata ctccaaaaac tcaccttttt ctatcatctc
     6361 ttcaaacttt tccttactta tgaagaaata atttactcca tcaacttctc cagttctggg
     6421 ttttcttgta gtagctgata cagaaagttt tattgtatca ttttctttaa gtagttcttt
     6481 gcaaatagta ccttttcctg cacctgatgg tcctgataca actagcaata atcctttttt
     6541 cacaaacatt taagcattcc ccttctaata ttgtattctt actctatatt ttgtatttgt
     6601 tctcttattt tttccaattc gctttttact tctactacta aattagttat atttatatca
     6661 gatgacttcg aacctatagt gttagtttct ctattcatct cttgtattaa gaaatctatt
     6721 tttctaccta tagagtcgtc tttaaatata gtatttttaa gttgccctat atgactttta
     6781 aatctaacta tttcttctgt tatactgctt ttatctgcat atattgcaac ttcttgtgct
     6841 aatctattct catctattat acttgggtca tccagtaatt cacttattct caaattcaat
     6901 ttttctctat aatcttctac aacactactt gaatattttt caatctcttc tatatgattt
     6961 tttaataaat cacatctcat ggcaatatct tcagctaatt ttttaccttc ttcacttctc
     7021 atttctctta atttgattaa tgcatcttca actgcttgat tcaacattga ccacaataaa
     7081 tcttcatctt cttccttttc ttctattttc acaatatcag gaaactttgc aacattcata
     7141 acgctaatat catctataat atcaaattta ttttttattt cttttagtat gtctatatac
     7201 tgagatgcta atccctcatc aaaatttaag tttacatctt ctttgcctaa taagtcaaac
     7261 tttatgtata aatctactct tcctcttttt acataatttt ttactaaatt tctaacttta
     7321 tcctctaaaa atgaaatttt tcttggaaga cgtatgttta tatctgaata cttatgattt
     7381 atcgttttac actctactaa aaagtaataa ttatcatcct tgtattctcc tcttccaaaa
     7441 ccagtcatac ttatagccat taattaaacc tccaaaatac cttcacatat ttttactgct
     7501 ggacctgtca tataagagaa atctcctaca tctattttaa ctgttccacc ttgagacgtt
     7561 acatttacac tactttctac catattaagt aaattgcaca ctattgctga tgcagtcata
     7621 ccagtaccac aacctaaagt atatccacaa cctctttccc aagtataaac atgtatatta
     7681 tacttatctt ctacctttac aaaatttaca ttcgtttttt caggaaatat tttattattt
     7741 tcaatctctt ttccatattt acaaacatca tctacatcta attcatctac tataactact
     7801 gtatgaggaa cccccatcaa catagaagta actttaagct ccctatctaa aacctttata
     7861 ctttcttgca caaaacgttc tttatttgta agtacaggaa cgtccttagc tataaaactt
     7921 ccttttccca tattgactct tatagagttt atttctctat tagtaagatt tacttttata
     7981 tcttttatac catctaatgt atctactgta aaactttctt ttttaactat acaattatca
     8041 taaacaaatt tacaaaaaca tctaaggcca tttccacaca tagctgctct tgaaccatca
     8101 gaattataat ataccatttc tacatctgca acttttgaat ctttcacaac taacaatcca
     8161 tccgctccta cagaaaatct tctatggcat actttttttg ctaaagcagt ataatcagat
     8221 ggatttatct ggtcgaatct tccatctatt gctataaaat catttcctat tccatgtagt
     8281 ttccaaaatt tcataagtat cgctccttcg taaattaaca cttaaactat tataacataa
     8341 aaaaattccc ataaacttat aatttatggg aaaagttttg taatgacaac tataatttga
     8401 ggatgtgagg gagagttgcc attacaatta gggtaattat attatgtttt taagctagtt
     8461 ctttctatgt ttatatatta atatttctat atgaatttta tgtatgtttt atttaaactg
     8521 tctatgaatt ttttaattaa acatctggta actagctata atccttctag tagatatcta
     8581 ttaacataca cctattaatt ctttaagtgt ttttaaaaca tagtctaaat cttccttagt
     8641 aatatctaca tgagttacaa atctatattc accattttcc attttattaa tttttatatt
     8701 tctttcaaaa aattctttta ttaaaacatc ctcttttata atatcttcac tcatcttaaa
     8761 aaataccata cttatatctg gattaacatt gtttacttca atgccatcaa ttttatttaa
     8821 ttcacaagct aaatattttg cattttcatg gtcttcattc aatcttaaag tcattttttc
     8881 aatggcaatt ataccagctg ctgcaactat acctacttgt ctcattcctc cacccataac
     8941 tttttgcttt cttctagctt ttgctataaa ttctttattt ccagcaagta tagaacccat
     9001 tggtgcagct aaaccttttg acacacagaa cataacacta tcacaacact ttgctatttc
     9061 tttagcttct acacccaaag ttaatgctgc gttaaataat cttgctccat ctaaatgtac
     9121 tggtaagtta tgtttctcag ctattttttt aatttctctt aaattatcaa gactcacaac
     9181 tgttccacct gaatatgcat tttctacaca tataagccct gtctgtggtt catgtatgtc
     9241 atctcttcta attgcctttt caacaagttc aggatttaga gctccacttt cgtcttcatc
     9301 tatagttcta agctgaactc cagctattac agcaggtgcc ccaacctcat gagtcacaat
     9361 gtgataacct ttaccaataa ttatttcttg ccctcttagg caatgagtaa atagagctaa
     9421 ttgattccca aaagttccac ttggaaggaa aatagcatct tcttttccaa ctttagctgc
     9481 tgctaattct tcaagtttat ttacagtagg gtcatctcta aagattgaat ctccaacagg
     9541 agcctttgcc atagcttctc tcatttcgtc agtaggcata gtaacagtat cacttcttaa
     9601 atcgataaat ctcatttttt caaccttctt tcatatttat atactataga accttaaaat
     9661 tgattttttc tgtaaagttc atatactttc atagcttata ctctacacaa taattatact
     9721 atatttactt tatatttaat ctactttaaa ttattaattt taaaaataaa gtttcctctc
     9781 taataaatat aaataattat acaatttaaa tattaatctc tattgtttaa attcccccta
     9841 gtcacaaagc gtatattatg tgtataatta atttactgac tcattttggt caaagaaagg
     9901 ggtgctataa atggcattag atggattagt tatacattca atagtcgatg aattatcatc
     9961 aaaacttaca ggtggaaaaa tagataagat acatcaacct gaagatgatg aggtaatttt
    10021 taatataaga aataataaag aaaattttag attggtttta agtgcaagtg cctcaaatcc
    10081 tagagtttat cttacaagta attatcaaaa agaaaaccct ctaaaagcgc ctatgttttg
    10141 tatgttactt agaaaataca tacaaggtgg aaatatagta gaaatttctc aaataggttt
    10201 tgaaagaata ataaaaataa gtgttgaatc tttagatgaa ttaaaagaaa agacagttaa
    10261 aaatattatg attgaaatta tgggaagaca tagcaacata ataataactc atggtgaaga
    10321 aaataaaatt atagattcta taaaaagagt cccttttagc ataagtagag ttcgtcaagt
    10381 tttacctggt catgattatt cattgccacc agaacaaaac aaactcaacc ctcttgatga
    10441 tatatctaag gatttgttta ttaaaaacct agaggaattg gaaggaccta tctttaagtc
    10501 tatatattct aaatttctag gtataagccc tattatagct aaagaaattt gctacagagc
    10561 aggtgtaaat caaaatgcta tcataaagga tatatctgat gaacaatttg attctttaca
    10621 caaagtattt tgtaatttat tcaatgatat aaattctaac aaatactctc cttgcattat
    10681 tatagataaa aaggtggata aggtagttga tttcagttgt ataaacctta cattatttag
    10741 cgatttatca tatataaata aggatagtat gtctcgtatt ctggaagatt tctatagaac
    10801 taaggatatt aaagatagaa ttaatcaacg ttcatctgat ttaaagaaga gtatatctgt
    10861 taagcttgat agactttata ataaacttaa aaaacaagaa gaagaattat cagaatccga
    10921 aaatgcagac atttataaga taaaaggaga attgattact tcatatattt atatggtaga
    10981 aaaaggtatg gaaagtattg aagttgctaa cttctatgat gaaaattgta atgatgtaac
    11041 aatagaatta aataaaaact tgacaccatc tgaaaatgct caaaaatact ttaaaaagta
    11101 taacaaaatg aaacatgcta aagtcgagat atctcatcaa atttccttaa ataaggaaga
    11161 aatagattac ttagaaaaca taattttaag tattgaaaac tgtgaaaatt tagctgaatt
    11221 acaagatata aaagaagaac ttgctaaggt tggatacatc aagactcaaa agaaaaacag
    11281 taaaaaggat actatcccat ctacaaaacc tcatgaattt gtttcttctg atggatttaa
    11341 aatattggtt ggtaaaaaca ataagcaaaa tgactatctt acactaagac ttgctgataa
    11401 tgatgactta tggatgcata caaagaatat tcctggttct catgtaataa taaagtgtgc
    11461 tggtaaggaa gtccctgata atactgtatt tgaaggagct atgctagcag cattctttag
    11521 taagtcaaaa ctttcttctc aggtgcctgt agactatact aagagaaaaa atgtgaaaaa
    11581 acctagtggt tctaaaccag gtatggttat ttatgaaact aacagtacta tttatgttac
    11641 tcctgaagaa gaaacagtag ccaagcttaa ggttaaatct gaataaattc tgcactaaat
    11701 acaaaataaa tgttagacaa ctacacatta tcattctaaa taaaaatagt ggccttctta
    11761 taaagtacaa taataaggcc actttctaat tagcttactt ttaattttct aacaaaatac
    11821 ttttctgttt taataactat acttatattc ccaacaaata ttttcagtct tcttatatag
    11881 atgtaattaa aaagttaaca ctaaaagcta ttttttaatt tattgatttg ttttagtaat
    11941 tcattatatt ctgattcata caattctttt tttagattat ctttttctat agataacata
    12001 gatataacag ttgtaagtct attttctaat actagaagat tttctttttt gatttggttt
    12061 tctctactag gagttttttt atttctgcta attgtatatt cgtcataatt acaatcaaac
    12121 tcccttattt tagcatcttt tatctctata atatagttac aaatatgtga tacaaatact
    12181 ctatcatgag acactataag catagtttta tcagtattta tcagtgcctt ttccagtgct
    12241 tccatagatt ttatatctaa gtaatttgtt ggttcatcaa atactaaaaa attattatct
    12301 tccaatatta ttttacatag tgctattttt accttttctc caccactaag tacttttact
    12361 tttttgtaga catcatcacc tttaaaccca aataggctta agtttatcct tataaaagat
    12421 tcatcaaatg aactattaac tttagtattg tatagaacac tcttttcctc atctaataaa
    12481 ctttgatttt ggtcaaaata acctactttt accttgttat ttatttttat attgtcattt
    12541 ttatcagcca atatttcttt tatcagagta ctttttccac acccattatc acctaataat
    12601 gctatctttt tacctctttt tatcttaaag gaaacattat ctaacaggag cttattttct
    12661 aactttagag tcatatcttt tacctctact agatttttac taattatctc cataccatct
    12721 tgaatcttaa tgttcatttc tttagaaact ttaggtttat tctttacctc aagtttatca
    12781 attctgcttt ttatagcctt cacatttgag tccaactttt tcttattagt ctgaccaccc
    12841 attttgtgca acctagcttc tgaattaccc attctttttg gtgtttttct tatgccatca
    12901 cttaaagctt ttttatataa aagagctttt tcaagccttt ttttctcttt aacatattct
    12961 tgatactcaa aatcagctct ttttacttct tcatcttttt gcattaaata atcagtgtaa
    13021 ttgcctttgt aagcttttag ttttccatct tctagttcta atatagttgt acataactca
    13081 tccagaaaac gtctatcatg agatattaat aataatgctc cctcatgcct tttaaacata
    13141 tcttccaaaa ccccaatgct ttttttatct aagtttgaag ttggctcatc agctattatg
    13201 atatttttct tattactcag cgcatctgct atttttaact taaccttttc ccctcctgat
    13261 aagtgttctt caaatttatc tggagcattt aaaagactct tctccttact atgactacat
    13321 gtttcaatat tattttcact ctgacttata taagaatagc tttctgtaag gtaagtatat
    13381 ccttcatcaa tttctatttc tcccaatatt gcttttaata atgttgtttt tccttgacca
    13441 tttgaaccaa ctagacctat tttgtcattt tctaatattt caagtttatc tatatccaaa
    13501 attaatttat ctgcataata tttctttaaa ttctctactt ttactaacaa cataaaaaat
    13561 ccctccttaa cacacttaaa tataaggaga gattctataa ataggcataa caaataaacg
    13621 tatagactat aatctactct ccttaattta agcattgcaa ataaagccac caaaataggc
    13681 ttttcaaaaa ttacaatact ttaaattata aaaaatagat tatattctca ttgtcctagt
    13741 taccttgccc tttctttcat ttatttttaa tcattatatc aacattaatt acaaatgtct
    13801 atagaaaatt aactaatttt agttattcaa tatacaactt attctttaaa tttattcttc
    13861 ttcaacttta aagtcctgat tatttaaaat tctgtttaat acaattccaa caattgcagc
    13921 taaactaaga ccagttatct ttactgtact tgttataggg attccaatag aaataccttt
    13981 attagataaa tatgtggttc caagacctat taataatata actgctatga ttaatatatt
    14041 tattttactt tctacacaag aactgtccct tatagttttt aatcctacat aagttatcat
    14101 cgaaaatagc attatactaa ttccacccat aactgaacct ggtatacttt gaagaaatcc
    14161 accaaacttt cctacacatg caagcaatat agcaaatatt gctgtacgtc taagtattga
    14221 agggtcatag tttttagtta tagcaagtac tgctgtattt tctccataag ttgtatttgc
    14281 tggtcctcct aaacaccctg caactatagt cgcaaatcca tccccaagta atgttctatt
    14341 taatccggga ttttctataa agtttttacc tactacagca ccattagtag tcatatctcc
    14401 tatatgttcc ataaatacag ctaatacaac tggagatata atcacaattg ctcctaaact
    14461 gaacttagga actgtaaagt taggtattgc aaataaactt gcttctgtaa tagtagttat
    14521 atctactaat cttaatatca aacagattat atacccagaa aaaactgcta ttaatattcc
    14581 taattgtttt ataaatcctc taccaaactt attggttaaa attgctattg ctaaagttat
    14641 agatgctatt acaaaattgg ctgaagccat actaaaagct gttggcaata aatttaatcc
    14701 tataactact atcatagctc ctgtaacttg tgcaggaaaa tataatttaa ttttatttac
    14761 accaactatt tttactataa tcgacattat aatataaatt aatcctgcta ctattatacc
    14821 accttgagca tatgctaaat ctccaccata tgcctcttta gctgctagta taactggtat
    14881 aaacgcaaaa cttgaaccta aaaaggcagg tacttttcct tctgtacaaa aatgaaatat
    14941 caatgtacca acaccagcac agaaaattgc aactgacgga ttaaaacctg tcaaaatggg
    15001 aactaatact gtcgctccaa acatggctaa tagatgttgt aaagacatta tcgttttttt
    15061 cataaaaacc tccaaaatta ttatatataa ataaatcctc tgtttaatta atatctttta
    15121 attaattatc attccctaga ttctattttt taacctctat atacttatta tataatttat
    15181 cattatttta cattttacga catcaaaaac tcaaataaag gagaataaaa actacattaa
    15241 gactcacatc cataaaatta taatatttat tttgtgcaat ctatgtaagc tttatcctcc
    15301 tccctgacta ttcaatgcta agcttttata taatacttaa aaataattat tctttgattg
    15361 taactgaatc ttctccatca atttctaata aactgacaga tataatttca tgtctagaag
    15421 taggtacatt ttttcctaca taatctgctc ttataggcaa ctctctatgc cctctgtcca
    15481 ctagcactgc taattgtatt gatttaggtc ttcctatatc tataatagca tctagtgaag
    15541 ctctcacagt tcttccagtg tataaaacat catcaactaa aactactgtc ttatcattta
    15601 cattaaaatc taaataagtt cctttaacta caggttcaac atgaattttt tccaaatcat
    15661 ctctatacaa agttatatca atatctcctg tatctacttt tgttccttct atttgttcta
    15721 ttttcttaga aattctattt gcaattggca cacctctagt ctttattcca actaaaacta
    15781 gattttctac acctttattt ctttcaatta tttcatgact tattctagta attgctcttg
    15841 ctattgcctt ttcatccatt aattgagctt tttctaccat atcataatta ttgtcaaaga
    15901 tataatatat ctatttgaca aatctactcc tttcttttaa ttttttataa atttattttt
    15961 atttaaaatt tcaatttaaa caacaaaaaa acttctcatc acaagacaag aagctcttat
    16021 ataaaaaata tacagtgcaa tttagcaatt gtcttacgat atctctgtat cgtttaaaga
    16081 taatttttta atctatcaaa tcaaattata tatttatatt tcaaattttt caagcttttt
    16141 taaacaaaat actttaatat aagatatttt atttaaattt cactaaattt atttacttat
    16201 ataaaaacaa aaaatgattt ataagtatta tacaaatggt tattttatct ttttaataac
    16261 ttccttaaaa tactcaggta attctgaatt aaattcgata tattccttag tagtaggatg
    16321 tataaatccc aacttcttag cgtgcaaagt ttgtcctttt aatttaaatt tagtttcttt
    16381 tggaccatat acataatcac ctaaaagtgg gtgatttata gacaaagcat gaactcttat
    16441 ttgatgagtt ctaccagttt caagcctagc tctcatatgt gtaaacttat caaatctttc
    16501 tacaacttca aagtgagtga ttgcattttt tccatctttt actacagcca tttttaatct
    16561 atccttagga tttctcccta taggtttatc aacagttatt ttatcttctt taaccacacc
    16621 atagcatata aattcatact ccctagttat agtatgttcc tttaactgtt ctgctagaaa
    16681 attatgtgca taattgttct tagctatcat aagaagacca gaagtatctt tgtctattct
    16741 atgaactata ccaggtctaa taactccatt tattgatgac aacttatctt tacaatgata
    16801 taaaatcccg tttactaacg tattttcata gtttccagga gcaggatgga caaccatatc
    16861 ctgaggttta tttattatta agacatcatc atcttcatat acaatatcta ttggaatatc
    16921 ttgaggtaaa acttctaata ctttaggttc tggtatctct ataacaatat tatcttcctc
    16981 tttaaccaga tatctagctt tttctacttt attattaacc tttacttttc catcttttat
    17041 aattttttgt atataacttc ttgacatatc acctaattgt tctgctagat aaatatcaag
    17101 tctaacttct tcttcctctt caagtactaa aaactctttt acttcatcca ttatatcacc
    17161 tacttttact ttcaaaaaat aaaacatata tacataaaaa cacagttcct acaactacaa
    17221 atacatcagc tatattgaat acatattccc atataattct aaagtcgaag taatctacta
    17281 caaaacctaa tcgtactcta tcaattagat ttcccaatgc accagatata attaatataa
    17341 ttcccaacct tccaaatata tgtactttcc ttgtattaag atagtatagt ccaaatactg
    17401 ttgcaagtaa tgcaacaatt ataaatatcc attgattatt ttgtaataat ccaaatgctg
    17461 cacctctatt ttcgacataa gttaaatgaa atacattatt tattattggt attgttgata
    17521 catccaccaa attattcaat acccatattt tagacagttg gtctaaacct atgagtagaa
    17581 ttattattaa tatatatagc aatgattttt ccccctataa attatttaaa aaccaatttt
    17641 atttgaagca cactattatt attatacaaa aaaaatgctc taaagagcaa ctttattaaa
    17701 attgtatttt cttaataaag ctagaacaaa atttaaaata gtactacttt ttttatattg
    17761 tagatttcta catatttaaa atcatatcat tatgtataaa aaatttaaaa ataccatact
    17821 aatcaatatt ttttgtgtat tttatagatt aaagattaat gatgtctgta atttaaactt
    17881 acaatcacca ctaatcttca aacaaatatt tttacttttg cacttgctta tttttatcta
    17941 aatgttgtat aagttcagat actgttacga atttatatcc tctctctttt aatttaggaa
    18001 ttactacttc taaagcttgt atagtttgtg atttatcatt tcttttagtg ttataatcat
    18061 gtaaaagtat tatattacca ttttggacct ttgtaaggat agtatttata attgcatcta
    18121 ctccagggtt agaccaatct ctagggtcta agtttgacca aagaattata ttcatgcctt
    18181 tagaaactat aatatcacag acacttttac tcatggctct atatggaggt ctaaatatct
    18241 ttggttctac tcctattaca ctttttataa tattttgcgt atctgttatt tctttttcaa
    18301 cactactata actatttttt gcaacattta tatgtgtaaa tgtatgattc cctatttcat
    18361 gcccctcatc atattgtctt tttaataatt ctttgttata ttctactttt tctccaacta
    18421 caaaaaaagt tgcttttaca tcatttttct ttaatatatc aagtacttgt ggtgtaaaat
    18481 cctcatctgg accatcatca aaagtcaatg ctaaaatttt ttcatcctca tttccttttt
    18541 ttattattac atcttcataa tcgtctttta tattgtcatt taaactatat gcttcttttg
    18601 aactatcttg attatgtgta ttatgattat atgtatatac acctactata gataataaac
    18661 atatacatat accccatatt attttcttca tgtcattacc tcttttctaa ttcattctag
    18721 caatatattt tataatgtca gttttttaat ctctcaatat tccattttta tttaaatcag
    18781 ttaaatttgt taggtcttct gaataaaagt tagaatcttc tacatcaggg ttttgcattg
    18841 catcacctaa taaattattt tctttctttg cacaatcatt acataaactt gtatctggca
    18901 atatatccaa tctttctaaa tctatttgat tatgacataa atcacaaatc ccatatgttc
    18961 cattctctat tttagataat gctaagtcta tttgatacaa tcttccctct tcatgattta
    19021 ttagactgtt ttgcatattt tgcatatata aatctgttcc catgtctcct atatgattat
    19081 cataactaga taattctcct gatgaatact tttcacttgt atgtttagtt gtatctccaa
    19141 ataaagtatt atctttcata tcctctacta aattagttaa attttctctt tctttcaata
    19201 atttatcttt atatttttta gtatccattt ttaacacctc taattttaat atttacatca
    19261 taaaaatagt atgtggaaaa gtaaattaaa catacaataa aaataagatt tgattcgaaa
    19321 aatggaatgt ctcaaaatga aatttttagt tcatttaacc ctctttttta tatgaaatca
    19381 aatatatttt tatcctttca gcaataaaaa agagacttat ctctattttg agatagtctc
    19441 ttctttgaac ataatttaaa tacaattttt taatttttat agttattatt ttactaactt
    19501 gccaatttat gattattttg ttctttaata tgttttgctt ttttaaagaa taaaagacca
    19561 agaattattg ctacaataac acctattcca ttagatatat taactggtag tctaaatcct
    19621 tcttttgctt gcattatata actaaatgtt actacagtca taaaaagtgc tggtaatgta
    19681 gttatataat ggtttttatt tgctttgact aaatatgcag aaccagtcca caacattatc
    19741 attgcaagtg tttgatttgc ccaagagaaa tatctccaaa ttatattaaa atctataaat
    19801 gtaagagcaa caccaactgc gaataatggt aaagctatta aaaatctatt tttgactgca
    19861 tcttgattat aattcattgc atctgctatt gtcagcctag cacttctaaa tgctgtatct
    19921 ccagaagtta taggacatgc cacaactcca agtatagcta aagctcctcc aataggcccc
    19981 attaatccaa cagatactgt atttactact gttgctgctc caccatcatt aaatatcaca
    20041 ttaagttgag gtatgccatg gaaatatgac atggctgctg ctgcccaaac taaagctatt
    20101 ataccttctg caaccattga cccataaaat actggtcttg cctcactctc tttctcaaca
    20161 catctcgcca taagtggtga ttgagttgca tgaaaacctg atattgctcc acatgctatt
    20221 gttataaata agtatgggaa taaaggtgtt ccatttggat tcatattttg taaatttata
    20281 tttggtatgt tatacccctt tattattaat cctccagcta cacctactgc cataattaat
    20341 aatgctgctc caaataaagg atatatttta ccaatcaatt tatctattgg taaaacagta
    20401 gccataatat aataaagtat tatcactcca acccatattt gtgcgtttac acctgtcaat
    20461 gtctttaata atcctgctgg acctgttaca aatacaactc caaccaatac taaaagaata
    20521 acactaaata ttctcattgc tatttttgca ccatttccta gataaaggcc taccaactct
    20581 gatgcagatg tacctttgtt tttcattgat agatatcctg aagcaaaatc gtgtactcca
    20641 ccagcaaaga ttgttccaaa cacaatccat aaaaatgctg atggtccaaa taatgctcct
    20701 tgtatagctc caaatatcgg acctaaccct gctatattta gaaactgaat taaaaagatt
    20761 ctcttccatt tcattggcac ataatctact ccatccctac aagctatagc tggagtttga
    20821 tttttgtcat tggttccaaa aaccttttct acaaaagtac catagacaaa ataacctact
    20881 ataagtacta ctattgaacc taaaaagctt accataaatt catcccctta gtttttatat
    20941 aattgcttta taaattttta tgagtttaag aaaactattt ccagttcaaa tagtaatact
    21001 tttttatgct aatataaata ttaattacat taagtgcata tttaagtaca tgaaatgact
    21061 ttatatatgc attaacagcc taaattcttt tactttgctt ctacttacag ttacttcata
    21121 atcactattt tttaatttta atatgtatgt attattaaac catggtataa tttcttttac
    21181 tttggttaag tttacgatat aagacctatg tgttctaaaa aatgttttat catttataag
    21241 attttcgact tctgatatgc cttcttttaa tataaacttt tctttctcag tatatataaa
    21301 tgtctgcctt tcattagctt cacagtaata tatatcatca atatctatta caactaattt
    21361 atcacctttc caaaggctaa ttttatgtgt tatttcttct atctcctgat ttactgcttc
    21421 cttcttatat ttgtataaat tactatttac attaaatagt tcaatatcac tcatctcact
    21481 tttttctaat ttattcagca tagaaataat tctctcttca gaatatggtt tcaatatata
    21541 atcaaataca tccaaagaaa aagcatctac tgcataactt tcatatgctg ttataaaaac
    21601 aattttaggt cttgatttaa actgatttaa tgtttttgca agtagcattc catcaagatg
    21661 aggtatattt atatctaaaa aaattacatc tattttattt tcctgaataa agtctaacac
    21721 ctctatacca ttggtaaatt cacttactac ctcgattcca cttttatttt caataaaata
    21781 tctcaattcc tttcttgctg gaaattcatc ttccactatt atagctctca tacttctact
    21841 cctttacata aaatactatc aaagtcccat tatctattct tcttatatta agccctcttc
    21901 catacatcag tttaagcctt aaatgtacat tataaagccc aatcttattt tctggcatat
    21961 tatcttgata tacattattt ataatttcct cacttatacc tataccatta tcctctattg
    22021 atattctcac agtattttct aatgaatacg gtaacttttt tatggatatt ttcactacac
    22081 cagctctacc actttctaaa ataccatgta ttattgcatt ttccactaat ggttgtataa
    22141 tcaaacttgg aatctttata tttacatctt ctatatcata agatacagtt agtaattctc
    22201 caaacctagc tttttctatc tctacaaaag atttaacttg ttcaatctct ttatttatat
    22261 caattaagtt atcactatat tcaagattat atcttagata agttgataaa tttattatta
    22321 actctctagc cttatctgga ctaaatctta taaatgaagc tattgtgttt agggaattaa
    22381 acaaaaaatg tggattaatt tgtctttgaa gtgcttttat ttctgcttta tttctcgctt
    22441 ctatatcttt tttatctttc tcaatcccct ctactataga tataacaaat cctattccaa
    22501 tctgcccaac tattaaaggt aaatatattc caccgattat ttgaattgct aaagaatgtg
    22561 gatatgatat taagtatata agtaatatag taaaactttc agaaatcatg cctaccaata
    22621 ttccatacat tactctatgt tgttttggta tcctcttata tagaaatcct gataatacac
    22681 ctgctagtat acttgataaa agacatggaa cagatgttat ccccccaata tccataaaat
    22741 atctatgaat ccctgaaaaa actcctgctg taatacctac catcggacca aataaaatgc
    22801 cacctgatac tatagatatt attctagtgt ttactattga ccccatataa tttataccac
    22861 tataagttcc aaatatggct aaaaaagtaa aaactaatgc tatacagacc aagtcattaa
    22921 aactatattc ttccttttga aatatctgct taaatatttt taactttgtt attagaagaa
    22981 acaatactac aataaggctt gtcttttcta ttaatgcgat taaaattgat gttttcataa
    23041 tcccccttta aaaaataaat ttttacacac tctaaattta ttatggttgt tataaattac
    23101 atatttgaag taatcctatc acaaaacttt ccacatttaa tcttttaatt aaataagaaa
    23161 tagttgtact tctattaaca actaatacta gctagcataa gcttgtaata acttgtagtt
    23221 attattaact ttacaattct agtatttaat tttatacttt agaaatctct tttcttagaa
    23281 ataatacttt cgaatatcat ataatacaat attatgtaga ttcttactca tacaacatat
    23341 ttctttaaaa attttatact attctttgtt caaatatctc atttgattta attgtgttaa
    23401 acacacatta tcatctctct gttatatatt tattatatag ccgtatatcg ataaagtaaa
    23461 taaatcccat atttaaaata atatgtttga caataaatta ttataatgta tatttaccat
    23521 aataatttgt caaccttgaa agttacttct ataattattg ttaaataatt
//
LOCUS       DAEOSP010000041        23036 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.041, whole genome shotgun sequence.
ACCESSION   DAEOSP010000041 DAEOSP010000000
VERSION     DAEOSP010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23036
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.041"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            338..1174
                     /locus_tag="KRH54_001331"
     CDS             338..1174
                     /locus_tag="KRH54_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG8578998.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1527..3104
                     /locus_tag="KRH54_001332"
     CDS             1527..3104
                     /locus_tag="KRH54_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG8578999.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSNFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            3097..4281
                     /locus_tag="KRH54_001333"
     CDS             3097..4281
                     /locus_tag="KRH54_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBG8579000.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIEKR
                     IL"
     gene            4299..4898
                     /locus_tag="KRH54_001334"
     CDS             4299..4898
                     /locus_tag="KRH54_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG8579001.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4922..5392
                     /locus_tag="KRH54_001335"
     CDS             4922..5392
                     /locus_tag="KRH54_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG8579002.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEVLVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
     gene            5751..6497
                     /locus_tag="KRH54_001336"
     CDS             5751..6497
                     /locus_tag="KRH54_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBG8579003.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKFYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            6558..7232
                     /locus_tag="KRH54_001337"
     CDS             6558..7232
                     /locus_tag="KRH54_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBG8579004.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLRCMMSCPWSL
                     RYANQK"
     gene            7601..8947
                     /locus_tag="KRH54_001338"
     CDS             7601..8947
                     /locus_tag="KRH54_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG8579005.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQNIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGRGNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIITLSGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDITVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNRNIDTLESDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            8974..10374
                     /locus_tag="KRH54_001339"
     CDS             8974..10374
                     /locus_tag="KRH54_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579006.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENRAI
                     ITAKSVRHCIKNICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAVICEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            10584..12020
                     /locus_tag="KRH54_001340"
     CDS             10584..12020
                     /locus_tag="KRH54_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBG8579007.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKDYEGYGSYKINNITIDQCGYCGFLFDSVKGSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGKETVYYYKHHEPDKTNQIQ"
     gene            12364..13308
                     /locus_tag="KRH54_001341"
     CDS             12364..13308
                     /locus_tag="KRH54_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HBG8579008.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            13477..14409
                     /locus_tag="KRH54_001342"
     CDS             13477..14409
                     /locus_tag="KRH54_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBG8579009.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKVK"
     gene            14514..14981
                     /locus_tag="KRH54_001343"
     CDS             14514..14981
                     /locus_tag="KRH54_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBG8579010.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(15225..16079)
                     /locus_tag="KRH54_001344"
     CDS             complement(15225..16079)
                     /locus_tag="KRH54_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar uptake protein"
                     /protein_id="HBG8579011.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYMENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            16245..18209
                     /locus_tag="KRH54_001345"
     CDS             16245..18209
                     /locus_tag="KRH54_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG8579012.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSYTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            18209..18670
                     /locus_tag="KRH54_001346"
     CDS             18209..18670
                     /locus_tag="KRH54_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8579013.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            18673..18993
                     /locus_tag="KRH54_001347"
     CDS             18673..18993
                     /locus_tag="KRH54_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG8579014.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            19009..20109
                     /locus_tag="KRH54_001348"
     CDS             19009..20109
                     /locus_tag="KRH54_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG8579015.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            20229..22829
                     /locus_tag="KRH54_001349"
     CDS             20229..22829
                     /locus_tag="KRH54_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBG8579016.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INIQLPALGYKVVSFELSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLKGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRKNGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKELEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         9063 a         2364 c         4059 g         7550 t
ORIGIN      
        1 ttagtacaaa gctataatct gaatttcaat aaatatatat ataattaagt tgaaacactt
       61 gaaaatactg gaaaaaattg aaaatagttt ttgtaatata aaatgtcgat tgattttaaa
      121 taattaaata tacataagaa ataattaaat atatttcaat gaaaatatga taaaatatga
      181 caaattaatt gatgacaata aaaaatattt tgttattgta gtattatagg gtaaattgca
      241 aaatatatac gaaagtaaaa ttttaaatat tgacattaaa ttaaatatac tatactatgt
      301 aagtgtgatg agagaatttt tttagagggg atttatagtg aatataaaaa agatatttaa
      361 taacaatgta gtagtaagct ccctagaaga tggcacagaa ataattgtca ctggtgctgg
      421 agtaggattt aagaaaaaag taggcgattt gatagatgaa aatttgattt ctaagaaata
      481 ttttgttcaa gacgatcaaa gggacaaata caatcaaata cttaataaga cctctattga
      541 atactttaaa atttcagaag aaattataga aaaggctaat gaagttttaa atactcaagt
      601 gaatgattca ataatattag ccttaactag tcacatagaa tttgctgtcc aaagagaaaa
      661 acaaggtata aaattaccaa acttaatatt gaatgagaca aaacaacttt atagggaaga
      721 atttgaattt ggattatggg caattgatga aattgaaaaa aggataggaa taaaattgcc
      781 tgaagatgaa gcaggatata ttgctattca cataatcaat ggtttggaaa attctcaaaa
      841 agatgaaggt attaatatac tagagttttc aaaacaagtt attcagataa ttgaagaagt
      901 acatggattt aaattggatg taaattcatt aaattatact agacttataa ctcacttaaa
      961 gttttttgtt caaaggattc tccgaaaaga aacttacaat gatagtgata ttgaagatat
     1021 gtataagtta gtgaataaaa attacaataa acctaaagtg tgtactgaag aaattgcagc
     1081 attggtatta gacagatttg aatataagat tagtaaagaa gaagaattat atttaatgat
     1141 tcacataaat aaaataacaa acagtggttg ttaggattgt aactgataaa gcaggcaaaa
     1201 cctaagtcat tgagcttaac tattagatta agttttttga tttaggtttt tttattagat
     1261 acaatcaaaa tacagacagt gacttttagg attgtaactg gttaagcagg caaaacctaa
     1321 atcattgaat ttaactatta ggttaagttt tttgatttag gtttttttat tagatacaat
     1381 caaatacaga cagtggttgt taggattgta actggttgag caggcaaaac ctaaatcatt
     1441 aaacttaatt attaaattaa gttttttgat ttaggttttt ctataaattt gattatgttt
     1501 ttagataaaa ataggagggg aaaattatga aaaaaaataa gttaggttta tggaacttct
     1561 tccaaatgtt aggaaaaacg tttatgttcc caatagcact acttagtgtt tctgggatga
     1621 tgctaggact tggggcaggt tttacagacc ctaagatgat tgaaatggtt ccatttttag
     1681 ggaatgaata tgttaactta gttttaaact tcatgttaac aataggatta tttgcattta
     1741 acaacttggg agcattattt gcaatggcaa ttccactagg attattaaaa aaagaaaaag
     1801 agtttggtgc tttttcagga ttagtaggat ttatagcaat gcatataggt acaaattttt
     1861 atttaacaag agctgatttg ttagtaccag cagaacagat gtcaactaat ggtcaagcta
     1921 tgattatggg tatacaaaca tataatacaa gtgttttagg tggtattgta gcaggattaa
     1981 ttgtttatgc aatatatgat aaagttagta atttaaagat accagaatca ttaggattct
     2041 acagtggacc aagattagtt ccaattgtta gtttaatagt tatgagtata gttggattat
     2101 taatacctat tgtttggcca ccatttttca atgcattcca aagtttagga agatggattt
     2161 cttcagcagg accattagga tatttttcat atgcagtggc agaaagagtt acaattccat
     2221 ttggactaaa tcatcttgta acatctgtat tccgttttac accaataggt ggaactgctg
     2281 ttattgatgg agaaacgtac tttggtacat tgaacatgtt tatggcatat gtagaaaata
     2341 atcagattat acctcttgat ttagctggta aaatggaaca aggtaaattg atgattcaat
     2401 atggattagc tggtgctgca ttggctatct accgtacagc aaaaccagaa aatagaaagg
     2461 cacttaaagg acttttaata tcaggagtgt tgacagttat tataggtggt ataagtgaac
     2521 caatagagtt tctattctta ttcatatcac cagcattatt tgctttccat acatttatga
     2581 atggtctagc aaacatggta cttccatact taggcgtgtt gatgggattc acaggagatt
     2641 taattgcatt tataagtttt ggtgtgttaa gaggaacagc tacaggatgg ccaatagcag
     2701 tattagtagc agttttatat tttgcaatat actattttgt atttaagtgg gcaattatta
     2761 agtttaatat aaagacccca ggacgtgagg ataaagaaat aaaagtatct gaaggaaatg
     2821 gttcaaattt caaaaatttg tctacatata aaggacaaca aatgatatct gctttaggtg
     2881 gtcaatctaa tatattatca ctagataatt gtgtaactag attacgttta aaattacaag
     2941 atgtatcatt aataaatgag gatgcaatta aagaggctgg tggtatagca gttgttaaat
     3001 tggatgagca tacaatacaa gtaattatag gaacccaagt gtattcttta agaaagcaga
     3061 tggataaagc tatggaaggt catttggagg catgtaatga ttaattttga taaaaaagta
     3121 gaccgattgg gtacatattg tacccagtgg gactatgtag aagatagatt tggtaagaag
     3181 gatttgcttc cttttacaat ttcagatatg gatttagagt ttccatttga aataacagag
     3241 gtgttgcagg aaaggttaaa tcatagagtt ttgggataca gtagatggaa acatgatgat
     3301 tttagaaatt cagttacgaa ttggtatatt aaaagattta attgtagcgt tgatagtaac
     3361 tggatatatt atagtccaag tgtaatgtat tcaattagca aacttattga gatgttaagt
     3421 aatgaaggag atggaatcct tataaatact cctgcataca atgcttttta tgatgtgata
     3481 ataaataaca atcgtaatat tataaagtca catcttgtaa ataggaatgg attctattca
     3541 attgatttta atgattttga gaaaaagtgt aaaaaatcta agatatttat tttatgtaat
     3601 cctcacaatc caactggtag agtatggagt gagttagaac ttgttaagat gataggaatt
     3661 tgtaagaata atgatgtcaa gataatatca gatgatatac acatggatat tgtatatgac
     3721 aatagaatga cacctatctt aaatgttgca aaggactatt tagattctgt attcatttgt
     3781 acatcagcgt ctaaatcttt caatatacca gcattgactg ggtcatatgt gattatacca
     3841 aatgaaggta ttagaaataa atttgaaaat atcactagat accgtgattt tgtaaattcg
     3901 ccagcaattc tagcaatatt agccacaatg gtctgttatg atgactgcga atattggtta
     3961 gatgaactat taaaatacct taaagagaat cttaaatata cgattgatta tataaaagat
     4021 aatttagaac cattgaaatt ggtcatgcca gaaggctgtt actttgcttg gatagatttt
     4081 tccgaactta aaataagtgg tgagaaattc caaaaattat tgatggatat tggagaagtt
     4141 gctattatgt cggggtctgt atatggtgaa gaaggtgagt tttatttaag gttaaatgtg
     4201 gcatgttcaa gagaaaaact tgaagatggt ttaaatagga ttaaaaaaac tatggattat
     4261 attgaaaaaa gaattttata gtaggaaggg agatagttat gggatattta aaaacaatgg
     4321 ttgatgttat gactactgaa atgaatgcca taagatgttt aatagatgaa gcaggaatag
     4381 aatatgaaag tatagttagt gaaattgcaa attgtgaagg caaagtaatt tttatgggtg
     4441 taggaaaatc tgcacatata ggtaagaaat tggcagctac atttgctagt actggtacac
     4501 ctagcttttt tgtacatgca acagaggcag tgcatggaga tttaggaatg attgaaagta
     4561 aggatattac aattttaata tcaaatagtg gaaattctat ggaagtggta aactgtataa
     4621 aatatattaa agcaataggt tctaagacaa ttgcatttac ttcaaacaga aattcagtac
     4681 tggcaaaaga gtgtgattat gctttaatat atcctgcaaa ggatgaagca gaccatttaa
     4741 atctagcacc aacaacatca tctacaatta ctttggtttt aggagattct attgcatgtg
     4801 cattatctaa gagtagtaat tttggttctt cagattttta taagtaccat ccaggaggaa
     4861 gtttaggtga gaagttaaaa acagctaaca atggataaaa gcaaaaagga gagtagtttt
     4921 tatgttcaaa atgtttaaaa aaaaatcaat aaaatcaccc atatcaggaa aagtaataga
     4981 gttatctaaa atacaagatg gtgtattttc tcaaaaaatt atgggagagg gtgtagctat
     5041 agattctaca ggagacattg tatatgctcc aatcaatgga aagatagtag tagtagcaga
     5101 gactaaacat gcatttgtaa tagaattaga aaatgggatg gaacttctta ttcatgttgg
     5161 tttagatact gtaaatttaa aaggcgaagg ttttgaggta ttggtgagta tgggagaaca
     5221 agtgaaggaa ggaacaccaa tgttgaaaat taaccgtcct ttgattgaat caaagggtat
     5281 atcacttatt actccagtta caataactaa tcattcagag tacaatatga atacatgtaa
     5341 tgtaggcaat agtgttgaag gtgggaaaga tactgtaatt gagtttaaat aaagaactaa
     5401 aatacaaggt taatatttat aaatacctat aaacttatgt tttataagat tgattgtata
     5461 gatttgttgt ataaaaaaga actctttaat tataaattaa ggagttcttt tttattttgt
     5521 aaatattgtt tagatttata tagaaaaaca cttagtaata aatagaataa ataagtattt
     5581 atatagctaa aaatctaaat agttttgttt aaatatattt ttatattttg taatttcaaa
     5641 ttaataaata attagatttg ataaacaaaa aataaaaatg ttgattacat taataaactt
     5701 taatataata tatttatgaa aaatgtaaac tgtgaaatgg aggtagtaac atgaaaattg
     5761 aaaatctttc tattgatgat attaaacgag gctatatatt taatgatgat ttaaattgtt
     5821 atagttgcat taaatgtaca aaaacatatg aggtaggaga aatatataaa ataggtaata
     5881 gattctttga agcttcaaaa gctattgaac ttcatatgga attagaacat agtgattatt
     5941 tagagcaatt gcttaatagt gattcaaagt acaacacatt gactgaaaat caaaaacaat
     6001 tatttcaatt atttattagt gatttaccag ataaggagat agcaaaacag gttggagtat
     6061 ccacatctac tatacgccat caaaaattta ttttcagaga aaaagcaaaa caggctaagt
     6121 tctatttagc actatatgaa tctgtatttg aagataaatc taataaaagt agtactataa
     6181 taccaatcca tgaacatgct cgaatggtag atgaaagata tgtagtaact gaagaagaga
     6241 gaaaacacat tttggataca tcatttgaaa gtatgaatcc tttaaaatta aaagtatttt
     6301 cttcaaaaga aaagaaaaag gtcgtaattt tatctaagat tttggaacaa tttgaacgtg
     6361 ggaagaaata ttcagaaaag gaagttaatc agattttaga atctatatat gatgattttg
     6421 caacaattag aagatatcta attgagtatg gttttatgag tagaaataaa gaatgtacaa
     6481 aatattggtt aacataatta aaataataaa ttttactaat gtgtaatgtt atttttatct
     6541 agggggagga gtcaaatatg aatgatgaat taaaatctat tttatacaat aaaggtgtag
     6601 acattgttcg ctttgttgat atatctgaat ttccaataaa tcaaacacaa ggtttttcta
     6661 aagcaatttt attttgtata gggttatcaa aaaaatttat tacagatata tacaataact
     6721 tacctacaga tagtgatgag tttttggaaa aagaggagaa agtagaagaa ttagctgact
     6781 ggatttcaaa atatattcaa aataaaggat atagagcgta ttctcaatcg gagaaaaaca
     6841 atctagaaca tggatatttt gaaaaagcct atattaatcc agaaatgcaa tcagggatta
     6901 gtcctttacc acataagaca atagcaaata ttagtggcat tggatttatg ggaaaaaata
     6961 atttgtttgt aacagaggaa tatggatgtg cttttagcat gtgtacagta cttacagatg
     7021 ctccaatttc tgttgaaaga tatcctttga ttgattcaaa atgtatggat tgcaatgttt
     7081 gtgtagagaa ctgtcctgca aaagctatac atggaaatga atggacttta cctggaaaaa
     7141 gagaatctat tattgatgtt tcaaagtgtt tttgtgtatt gaggtgtatg atgagttgcc
     7201 catggtcatt aagatatgca aatcaaaaat aactttacta gagaaggtat taaattgaat
     7261 attataaaat ttttaaaatg taagattata taagattttt caaaataaaa atttgtatag
     7321 aacactcaga aatgagtgtt tttctattgg gattaaatta tattgataca aagcttaact
     7381 attttaatca ttatataaaa tttgtattaa aaaattaaga ataacttggt aatagtgaaa
     7441 atataatgag attataattg atttataata taactataat gatattgtaa aaatgaaaat
     7501 tgtcctctca aaattcccaa agagagtaaa agtcacttca gttatagtca aaaaaatttt
     7561 tctgtgaaaa gaatttagat ttgaaaagga ggaattaatc atggaaattg aaactaaaac
     7621 tccagattgg attaatgtac tagaattaaa cagtaaaata gatattactg ggaaatatga
     7681 tgtaagtaat atgatacaaa atattgtctc tgataagtca ctagaaggtt ccattatata
     7741 ttttccaaaa ggtaattact tatttgagaa aggtattaaa attagtcaac aaataacact
     7801 tcaaggagat tcttattatg gaagaggtaa tcaagtatca aatttagata aaaaacaatc
     7861 aatcatagga acaaccaatt ttataacccg aggagtttct aatatgagta ttattacatt
     7921 gtcaggaaca agtcaatgta tcaaaaatat taatttttat tatgatagtc atgatataga
     7981 gaaaataccg cctaaaaatg tatctgctat tacagaatat ggagaaacac aaggactatc
     8041 tcattttgaa catctgttta tttctggatt ttcagggata ggaattgaaa ttccatacta
     8101 ttcaactggt aatgatatta ctgttagttc atgtggtctt ggtatgaggc ttggagagaa
     8161 gtcaatgctt tctagtagca agatttacga atgtaaaaat ggaatggaga ttactactgg
     8221 tgttagctta aataatgtga gaatagaaga aataaaagaa ataggtatta ataataaagg
     8281 atttggtttt aacctgatta tgaatcttat ggttgatcaa tgtggatatt gtggatttat
     8341 gtttgaaaaa atgtgctata gccagataac tgccagaatt actagatgcg gtcagtatta
     8401 taaaagtgtt gattataatg cttatgagaa tatggaagat aggaaagaag aagcttattc
     8461 cattttttat ggagatattt tagaaaattg caatattgta ttgatgaata gaaatattga
     8521 taccttagaa agtgattcac ttaatgaaca taagatatat gtaatcaagg caaatgaaac
     8581 aaacaatgta atgctaacat gttatgctga agcagataac tttatacaat gtgcaaaggg
     8641 caacttgctg ttagaaaatg gtgaaaatac ttataaattt tataatggtg agatttgttc
     8701 tgtaggaggt gttcaaattt ctgatataga tgaaaaagat ttaatcaaaa taatagagaa
     8761 cactttgtat atagatgata aaggtaagta tttaatggtt cctagattaa aaaagaatac
     8821 tgtaatatat tcagtactag attctacaga agaaataaat aaattacatg gtggaatatg
     8881 ggaacagatt gatgtaaaag tattagctgg ggaatccatg tactattata aaaagataaa
     8941 agattaaatt atcaaactag gaggtagtga ttaatggaag agtctataat aattaaagat
     9001 ataagtggct ggattaatgt acttgattat ggaatttgta atgatgaaac aaaagatgta
     9061 agttttaagt tgcaagagat tatggatgct gtaccagaag attctattat atattttcca
     9121 aaaggaaaat acttatttga aagtggtgtt aagattaaga aacgattaac cttatgtgga
     9181 gattcttata ttttagcgaa tgttcctaaa aatactgctg gagtaacaca gtttactttt
     9241 aaaggaaaga aaaatcctaa aagtgaaaat agagcaatca taacagcaaa aagtgtaaga
     9301 cactgtatta agaacatttg cttttattca gatagttgtg atagagatat gacatctaat
     9361 catgaacctc caacagatgg aaaacctaga tttcatcata atgtaattat aaattataga
     9421 aatatatctg ctgttatatg tgaagataaa cagggagaac caggtcatta tgaaaatcta
     9481 tcttttagtg gtttttctgg aattgcactt gatatgccta ataattctac agcaaatgat
     9541 attacaattt ttacatccgg acttggaatt agtacagggg aaaattcaac aattgctaac
     9601 agtaaggttt ggggctgtgt tgatggaatg ataatttcta ctggaacttt tataaatggt
     9661 atgagggtag aagaaataaa aaaagttgga attaaaaata ttggtagagg tttaaatttt
     9721 attacaaata tcacaattga ccaatgtgga tattgtggag tttggtttga ttctatatcc
     9781 aatgttcaga tatcaggaaa tatcacaagg tgtgggcagt actattatga tgtaagctat
     9841 gataattatt taatgttgga taatagagtt aaagaagcat attctttatt ctatggtaat
     9901 ttattggaat caagtaatat agttttatca aataccaata aagataattg ggaagatttc
     9961 aaagaagata agcataaggt gtatcttgta gaagcacttg aaaccaagaa tgtactatta
    10021 acatgcaata tagatgctac tgactggatt gttaagtcag aaaaaggaaa tttaacgtac
    10081 aataatatgc gacaaactgt tatattttac gatggaaaac taagtagtat caatggaatt
    10141 ggtatatctg atgaagatta tggggataaa gtcaagataa aaaaaggaaa tatatatata
    10201 aatagtaagg ataaggatat taagatgcat aaaccagagg taggtgacat tatgtcaaca
    10261 attattaaag ataaaagtat tctttctgct cattatgggg gaacatggga gaaaattggt
    10321 gaaaaaatag agtttggaat aactgtacac tattataaat tgatagagaa ataagtaaat
    10381 acgtagtatt tgaatagatg tattttattg gagagctagg actatagttt taatgtaaat
    10441 ggtttggtaa tagcatggat tgaataatat aaaaataata taaaaaatta atttgattat
    10501 gtaaaattct atgaagtagt aaaactgtga ctttagtcat aggaggttca aactaagtaa
    10561 aagggaaagg aatatataat attatggaag gaagaactgg gattaaagat ggaagatgga
    10621 ttaatgtagt agaaagaggt attaaaaatg atggttcgta caacataggt cctgctttgc
    10681 aggatattat tgatgaaata gaaacagatt tttgtgtctt atattttcca aaaggtaaat
    10741 acctatttac aagtggtgtt aaaattaata aagaaattgt attgcaaggt gattctaatt
    10801 catctacagg tactacacaa tttataactc gtggagtaga aaatatgagt attattacat
    10861 tgacagggaa gaaacaatgt attaaaaata taaatttcta ttctgatagt tgtgagatac
    10921 aggtaaataa agaaccaccg acaaaaggta atcctaaata tcattatgag atgattatca
    10981 ataagaatga aaagaatgtt gagatgaaca atgtatcagc aatcctgtat gataacagta
    11041 agaatatgat taaaggtcta ggccattatg aaaatattta tataagtggt ttttcgggta
    11101 caggaattag gataccatat tactcaattg taaatgatat tacagtttca tcttgtgggc
    11161 tgggtattga tacaggaata gatacaatta tttctaatag tagaatatct aagtgtcaaa
    11221 atggaatgag gattaggact ggaactagca taaataatgt aagaattgaa aaaatacaga
    11281 aagttggtct tataagtagt caagttaagg attatgaggg atatggaagt tataaaatta
    11341 ataatattac gattgaccaa tgtgggtatt gtggtttttt atttgactca gtgaaaggta
    11401 gttatttttc tggtattata agaagatgtg ggcagtacta ttataataca gattatgaca
    11461 catatttaag tattaaagat agagtagaag aagcatattc tttattttat ggaaattatt
    11521 ttacagaatg taatattgat ttagttagtg ataacaagga taactgggat gatggcttag
    11581 aatataaaca taaagtttat gtatttaaaa cccttcaaat accaaaatta acattgagat
    11641 gcaatataga tgcctatgat tatatcgtta aatcggtaca tggaggaggt aatttaatgt
    11701 taaaaaataa ctttaacaat tatatattta ttgattcatc taatccagaa gttattaatg
    11761 gaattaattt taattatcaa aagaatggaa acttgttaag gttaaataat ggtaaattaa
    11821 gttataaaaa tggaaaggaa gaaacagcta tattcagacc taattatggt cttattgtag
    11881 catcaacaga ttcagaatgt gaacaaatag agactagcta tggaggaaaa tgggagaagc
    11941 ttggttctga agtaattgga aaagaaacgg tatattatta caaacatcat gaaccagata
    12001 aaacaaacca aatacaataa aatttgtatt atataatgat ataaagtttt aatatatagt
    12061 gtttatcttt aatgtaatat tggtatattt gtatagtatt aaatttgtta aaaagtaaaa
    12121 ttgtagggtg cttgaaaata gcaccttttt tagtttcatt ataaaattag ttttgaaagt
    12181 ttaggaataa tgtatattta tttttaaaga attataacct tattaaggat attttaaatg
    12241 ctagaaatga atcagtaaat catactggaa ttatcttaaa tgttttgatt aaattaagta
    12301 tttagagcta taattatatt aaaaatatat tagaacataa ttatattact atggaggtta
    12361 aatatgatag taagtgtgag tagaagaact gatatacctg ctttctatag taagtggttt
    12421 tttaacagac ttaaagaagg ttttgtttat gtaattaatc caatgaatcc aaaacaggtt
    12481 agtaagatag agttaaatcc acatactgtt gattgttttg tattttggac aaaggatgta
    12541 actccaatgt tatatgattt aaataaatta aaagactata aatattattt tcactatact
    12601 ataacatcat atggaaaaga agtagagaca tgtattttag ataaaagaaa ggtaatagat
    12661 tcatttaagg aattgtctag aaaaataggc aaagaaagag taattttaag atacgacccg
    12721 attttcttaa gtgaaaagta cacagtagat tatcatatca aagcatttgc gagtttatgt
    12781 aatcagcttg atggatttac agaaaaatgt ataataagtt ttatcgattt gtataagaaa
    12841 acaaaattta atactaaagc attacatata aagccaatag aaattacaga aatagaaaca
    12901 ttatcaaaag agatttctaa gattgctcat aagcatggga taatacttga aatgtgttct
    12961 gaagaatata atttaagtaa atttgggatt ggaaaaagta agtgtattga tgatagatta
    13021 atatcaaaaa ttataggatg tgaagtagag gtaaaaaaag atagtacaca aagagatatt
    13081 tgtggatgtg ttaaaagtgt cgatatagga caatacaata cttgcagaca ttattgtctg
    13141 tattgttatg ctaactttaa ttatgcaaaa gtagaagaaa actgtaggct ttataaggaa
    13201 gataataaat tactcataga tactttaaga gatgatgcaa aaataagcat tagagatatg
    13261 aagactatta aagtagataa gaataaacaa attagtatat ttgattaaaa taaatatact
    13321 aattacctta atatattaag aatatagata cttttgataa taaaattaaa cttaatagat
    13381 attttctata taagaaagat ggtcataata tgtcaatttg accatctttt tgatataatt
    13441 aaaacacaaa taatttagta acaaaggagg cagaacttga atttattaaa aaaatatcct
    13501 atacctattg caagtttgat tttgtcaatc tttactttag gtaacttatt acaatcttat
    13561 agtgaatcat tacgtaatgt aattggattt ataggattta ttttgtatgc aatttatata
    13621 gtcaaaataa tattacttag aaagggtgtt aaagagcaat tagagaaccc tttaatatca
    13681 agtacatttc caactataac aatggcaaca atgatatttg ctacatatat aaaaccatta
    13741 tctttagaat taagtattat gatttggagc ataggaattg tgggtcatct tgtgttaata
    13801 gtcttattta gtaagaagtt cttagttaaa ttctcaataa aaacggtatt tccatcttgg
    13861 tatatagtat atgttgggat agttgcagct agtgttacgg caccagcatt taatcaactt
    13921 ttgattggca aaattgcttt ttggatagga tttgtatctt atatagttct tataccagta
    13981 attttaaaga gagtatggat aatcaaggaa atgcctgaac catctttacc aagtatagct
    14041 atattagctg caccagggtc actattattg tctggatata ttaattcttt ttcagataaa
    14101 aataatgtag tattatactt gttattcata ttatcaattg tattttatat tatagttttg
    14161 ggatacttac cgaaattatt aagattgcct ttttatccga gttttgctgc atttacattc
    14221 cctatggcaa taagtgcaat gggtatgaaa ctaatgaatg gttatttaac taaatcaggt
    14281 cattttataa gttggatttc atatgcttct aagttagaag agataattgc tacagtgatt
    14341 attttatatg ttttagtttt gtttattata tatcttagta aatctgaaac tacatctaaa
    14401 gttaaataag aattattaaa tatgtgtatt taataggaat attgatataa atatatgaag
    14461 ataagataaa ccatagaaac ttaatttagc ttaattttat tggaggaaaa caaatgaaaa
    14521 caaaagaaga atatttaaaa gaagttagaa acatatcaga agaatacttt agaaaaggag
    14581 agtttttttg ctctgaagca gttctccaga caataaatga tgctttaggc caacctttaa
    14641 gtccagagat aactaagtta gcatcaggat ttccgatagg acttggaaag gcacaatgtc
    14701 tttgcggagc aatatctggg ggagagatgg ctttaggtat agtatatgga agagttcatg
    14761 gtgaagctat gaatccaaag atgtttgagc atgctaaagg tttgcatgat tatattaaaa
    14821 aagagtatgg tgcaacatgt tgtagagcta ttacaaagaa gtgggatggt gataacttta
    14881 tgtctcccga aagaaaagag cactgtatta aaataacagg tcaagttaca gagtgggttg
    14941 cagataagct tatagaagat gaaaaactta atatcaaata gtgcaatatt agttggtgaa
    15001 tttataaagt taaatttact tatagtataa gatacttgat attctcgtat tgatagaaaa
    15061 atttatagca tatgtattta atgaaatata cttatattta gagtgtggtt taatttcata
    15121 aatacatatg ctattatttt aagtatataa gcaaatttat ttaaataaat agaaacagtt
    15181 aatttatatg tacaaaacta tatatgatta gtgtaaataa atacctattt tataatactc
    15241 aacataaaga caccagacat gattagtatt ataccaataa aaatagcaag ccattctttt
    15301 ttgttcttct tttctttaaa aaatataatc cctcctaaag tggctactat cacacaactc
    15361 tgagagatag aaaaacttat tgtaactccc aatgtattag tcgttatgaa catacctaaa
    15421 tttgctattg accacaatat accagtaatt aaattgaata aaacattttc catatataca
    15481 atctctttag aaaaatactt gacaataata gatgaagtta tcaacatacc aacagattga
    15541 ggtaatataa ttctatatcc ttcaattaaa aatatttgat tagttacaac atataatgat
    15601 aaaaatagag atgataataa tacaactcca acacatttta acatattttt atctgtagat
    15661 tttttagttt gataatttgt gagaagtata ccaagtatta tacataatat tgataaagaa
    15721 ccaatcagta acattttaga actggtccat tcattaaaca agattacagc taataatgtc
    15781 gtaaaagtta attgtgaacc atttgaaatt ggcattactt tagatacact agcaatttga
    15841 attgctttaa actgcattaa ttgtcccaga ctccagaata tgccagaaat catacttata
    15901 caaaatgaaa aaaatgtaat ttcaggatgt ataaccagag ttattatagt ggaaaatata
    15961 aacgcactta tggatgtacc taaaagctgt tcatgaggag aacttttctt tagatttgca
    16021 actatgggca tcaatcccca tcccattaca ggaagtaaag caataattat tgtcagcatt
    16081 aaactatttc ctttcatatt aaatttagac agcttttaat ttatcatcta aaattaaagt
    16141 gttcaataca taaaaattac cgtatttaat caggaaatat tagtagtttt ttagaaaaac
    16201 attttccatt aaaataaaga caaagggaag gagtaaaatt gaaaatgtta actgatatga
    16261 ttcaaaatcg tgaaattaaa ataataaagt ttttattaaa agaaggtcat acaactgtaa
    16321 aagcaatagc agatgattta gatgtttcag aaaaaacagt ttcaaaatct cttaaagaag
    16381 tagaagcagt gcttaaaaag ataggtcttt ctttaataag aaaacctaag ataggtgttt
    16441 atattgatgg agatttaaaa aaaatacttc cttacctaga taataaagga agtcaaattc
    16501 ctacaaccag agaggagaga gttatctata tatattcagt acttttaaag agtaatgatt
    16561 atattactat acaacaactt tctgatgaac tatatataag tcgtggaact attgaaaggg
    16621 atttagcaga agttgaaaaa ctgctaaaaa acgaagggat ttccatatat aagaaaccta
    16681 gtaagggaat aaagttaaat ataagtgaaa gagataaaag acaacttact gctaagttta
    16741 tacataaatt ttggagtaga aactggtata ttaagcaaga gcaagaaagc ttttatcaag
    16801 catttgaaaa gatacaagca gatgtaaatg gtattttttc ggaagatgat ttatcaataa
    16861 ttattgatat tcttaggaat ttcagcagag aaagaaaatt tgagttctct gattatgaat
    16921 ttcagtcatt agttattcat ttggcaatag ctgtagagag aattagaaaa ggcgaatata
    16981 tagaagaaag tttgtatact ggtaatatag aaaataatca aatgtcaaat accaattatt
    17041 tagcaaaaca attagaagaa aaattttcaa tattattacc aaaatctgaa attggatata
    17101 taaatttaca tttagtagcg tctgaccaac atagagatac taatgaacta aaaaatactt
    17161 caactaatcc ttgtgagaca agtacaaata atatatcaaa tcttcgggaa attataaaaa
    17221 atacattgta tgagacagct tatgacaagg atttaattga tggattaact tcacatgtac
    17281 attcatcaat aaatagactg aaatatggac ttagtattaa aaatccatat gtaaatacaa
    17341 tcaaacaaaa cttttcttta tcatttgagc aagctttaga tttaaagaaa gtaattgaat
    17401 ttatatatga tattaggata aatgatgatg aatgtgctta tatagcatta cattttgaag
    17461 cttttagaga aagaatgaaa gaacttccac aaaatattag agtaattcta gtatgcagta
    17521 caggattagg ttcatctagg ttattgtctg caagaataaa aaaatacttt ccaatgataa
    17581 agattgaaaa agtactttcc gtacaagaat tagtatccag taaaattgat gcagatttag
    17641 taataagtac tatttattta gagttagaaa caattccaac catagtagtt agtcctattt
    17701 taaatgaaag tgatataaga accttggaaa gaaatataca aaatgtttca agttatactt
    17761 ctaagaagta taaatctttc agaaacttaa tctttgaaga aaatatattg ttgaatattg
    17821 aagcttcaaa ctatgaagat gcaattaagt atataacaga taacctaata aaaaaaggtt
    17881 ttgcaaaaga aggtatagct aaaagtgcaa ttgagagaga aaatttatct tacacttctt
    17941 tccaatcaat agctacacct catgctaatc ctagttatat taaaaaatct aatatatctg
    18001 ttgcaacgtt aaaaaatcct attacatggg gaaatgaaaa ggtggaagtt attttcttta
    18061 tttcattaga aaatgacaaa aacctagagc ttgatgaaat atatgaagtt ttttatgact
    18121 tcattgacaa taaaaaaaat ataaatgaca tattaagtgc aacttccaca aaggaagtgt
    18181 acgatttgct agtggaggag agtatatagt gaaaatatca gatgtattga aaaaagagca
    18241 aattgttttt aatcttgata caaatacaaa ggaagaaaca ataaaaaaat taagtagtat
    18301 atttgttgaa aatgggattg tggacaatga agaagaatat gttaagtctg ttttggaaag
    18361 agaagaacat tcaactacag gtattggtaa tggtattgct attcctcatg gaaaaagtaa
    18421 tgctgtcaaa aaatcttcta taatatttgc aaagactaag cataagattg aatgggatgc
    18481 tttagatgaa aaaccagtag atagtatttt cttattggct ataagcaata ttgataagga
    18541 tagcaatcat cttgtactat tatctaaatt agcaactaaa cttatggatg atgacaatgt
    18601 agatgcacta aaattagcca attcagaaca agaaataatc aatatattta gtgaagaagg
    18661 agagatgtag aaatgaaaag aaaaattata gcagttacag catgtgcaac aggagttgca
    18721 catacatata tggcagctca agcgttaaag aaagcttcaa aaaagatggg tcaccttata
    18781 aaagttgaaa cacaaggagc tacaggaatc gataatgagt taactacaaa ggatgcacaa
    18841 attgcagaag tagtaatatt tgcagtagat actaaagtca gaaatgctga acgttttgag
    18901 ggaaaagaaa tactagaagt accagttaat gcacctataa aggatgcaga gaaagtaata
    18961 aatgatgcac taaagttaat tgacactaaa taaatggagg gaaatataat gttgaaaaag
    19021 atagtctctg agttaaaaaa gcatgtctta actggaatct catatatgat tccattagta
    19081 attgcaggtg ctatgataat ggcgatatct cgtgtaggtg gttctttcta taatatacca
    19141 gatatatggg atgctaaata tgcagaaagt gctagtagta ttgttagatt attacatgat
    19201 ttagatgggt ttggaggtac tgcattagga ttaatgtttc ctgtaattgc tgcatttata
    19261 ggattttcta tagctgataa attagctatt gtaccaggtc ttgttggagg tatgattgct
    19321 aaagatatag gtgcaggatt tttaggagct ttggcagttg gtcttatagc aggatataca
    19381 tgtttgttta ttaaaaatca tgtcaaatta ccaaaatcag cagcttctat agtaccaata
    19441 tttatagtac cagtatttgg gacattaatc actgttgtat taatcaatta tgtgataggt
    19501 ataccttttg ccacattaaa tactggatta gaaaattggt taaatggatt atctggtaca
    19561 aatcagatat taatggcagc tattataggg gctatgatgg cagttgactt aggtggacca
    19621 gttaataaag cagctttaac aacatcactt gcattattaa caagtggaat ttatgcgcct
    19681 aacactgctg caatggtagg aatagttata ccaccactag gattgggact tgctactata
    19741 ctagcaaaac aaaaatataa taaacagtta cgtgaagctg ggaaatcatc tcttataatg
    19801 ggtttaattg gagtaagtga aggagcaatt ccttttgcag tagaatctcc attaaaagta
    19861 ataccttcat gtgtaattgg aacagcaatt gcttcagcta tggcagttgg tttaggttca
    19921 gtaaatgcaa ctccaattag tggattttat ggatggttta cagtagagaa ttggccaatg
    19981 tatgtgttat caatagcagt tggaacagta attgtagctg gattagttat agtattgcgt
    20041 ggaaatccag tgattgagga tgatgaattt gaagatgatg attttgatga agcagagtgg
    20101 gaatcttaaa atttaataac tatttaagac gtgttataag ctatattcaa tatgaatctt
    20161 atactaaata aagtgttaaa tattcagttt agacttaaat ataaaagata aagaacagag
    20221 gagaataaat gataaaagca catattgtta atcatacaca ctgggataga gaatggtatt
    20281 ttacttctgg agatgcatta gtacttagtg aacaactatt tacagatgta attgatgaat
    20341 tagaacgtaa tccagatgta agttttgtac ttgatggaca actttctatt ttggatgatt
    20401 atgtacaatt acacaaagaa aaaatagaaa tcattaaaaa gttaataaaa gaagatagac
    20461 tacatattgg accatggttt actcaaactg atgcattctt tgctagagga gagtctattt
    20521 taagaaactt aatgattgga atttttgaaa gtaaaaagta tggtaagtat atgaaaattg
    20581 ggtatttgcc agatacattt ggatttaatg cacagatgcc tatactatta aagcatgtag
    20641 gtcttgataa tatattcttt tggagaggta tacatcttgg taaacaggtt caatcaccat
    20701 attttaagtg gaaaagctta aatggagaaa gttatatata tgcagtaaat atgccacatg
    20761 gatatggaac tggtatgctt ttagagcctt cattaaagta tgttaatgga cgcttagacc
    20821 cagctattga ttttattaaa tcatatactg atgtagatga ggtgttaata ccttctggga
    20881 atgaccagtt aaatataata ggtgatttca aaaataaaat taaagcgatt aatgatatcg
    20941 gaaagtattc atatataacg agttcatatc aagagttttt aaaatatata aagtccatag
    21001 aatcactaga aagctataga ggtgaattta gagaacctgt attagcaaga atacataaat
    21061 caattggttc tagtagaatg gatataaaat tagcatgtga taaactagaa aacaagctga
    21121 ttaaaagaat agagccatta ttagtaattg ctaaaaagag taaaattgaa ataagcaatc
    21181 agttacttat aaatacatgg aaaaaacttt tggaaggaca agcacacgat agtttagctg
    21241 gatgtgtaac tgatactgta acagatgata ttcttcaccg tataagagaa gcaaatgaaa
    21301 tatgtgacag tattgaaaat actattgtaa aaaagatttc agaagggctt aaattatcta
    21361 aaaatgatat tttagtattt aatacagaag ctaagcgttt taatggatat aaagagatac
    21421 aagttgtaag tgatagtaaa aatatttatt ttaaagataa tttagatgct actataattg
    21481 aagaaactta tgtaaaaccg agagaaaatg tattagaaga aacacctgct ggtaatatat
    21541 ttattgaaga accaggatat tatatattaa aagtaagaat aaatatacag cttcctgcac
    21601 ttggttataa agttgttagt tttgaattaa gtgataaaga aatggtgtca cttgataaat
    21661 ctaatgacac ttttatatct aatgataatt ataagataat atatgaagat ggaagcttaa
    21721 atttacaact taaagatgga acttacatat caaacttttt aacattaaaa gatagtggta
    21781 atgcaggtga tacctatgac ttttctccac taaaaaatga tgaggatata aaactttcgt
    21841 ttgataaagt gtatacagaa aaagctttag gatatgaaaa aatggtggtt aaaggtacta
    21901 ctttactacc acttacatta gaagatagaa aaaacaaata tcttaaaggg aaattaaatg
    21961 taaaagtaag tattgtctta tctaaagaaa acccattgat ggatgtaaaa ctttgtgttg
    22021 ataacacgat atataatcat agactacgtg ttcatataaa aactgatata aaagataata
    22081 aaaacatagc atcattgcca tttggatata taacaagaaa aaatggagtt ttagacaact
    22141 gggaagatat ttacagtgaa atgcctatag atttagaacc tttggaaagt aatattactc
    22201 taacaaacca aaatagaagt tgtactgtat ttactagagg aataaaagag tatcaacata
    22261 ttgaagatga aattgcattg accttacttg ctactacaga tgaactagga aagcctgatt
    22321 tattatatag accaggacgt gcttctggtg atacaactaa aaaaggtcat ataagaatta
    22381 aaacagataa agctcaaatg cttaaggaat tagaattttc atttgcaata tatatggatt
    22441 ctaaatcatt tgatgaattg gaaattgcga atttaaccca caattatcta agggaatgtg
    22501 taaattatca attacaggat tacaatttct ttttatatcg tatagataat aagattcaaa
    22561 aatcaattgt caataaaact gtaagtagag aattagaaat aatatcttta ccagaaaact
    22621 atcttatatc agcatgttat ccttcttatt acagtaaaga taaatttata ataagagtgg
    22681 aaaatccaag tagtaataga atgatattgg atgagcaaat atttaaaaat agaaatggga
    22741 aaattgtaaa tgcaatagaa gatgtagaag aggaacaggt atatgaaata gcaccttttg
    22801 acgttatttc aatattattg gatttataag tgtaaaagta agtttattgt ttaaagtctg
    22861 acagatatta atctgccaga ctttttaatt tgtataaatt tgggtaaata ttaatgaatg
    22921 gttttagtat ttaagtatga atttagtatt taatatagaa tttattgaag tagaataatt
    22981 agtttaacag gataagcagt ttaaataaag aattattata ttagagtgat tttata
//
LOCUS       DAEOSP010000042        22835 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.042, whole genome shotgun sequence.
ACCESSION   DAEOSP010000042 DAEOSP010000000
VERSION     DAEOSP010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22835
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.042"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            182..1618
                     /locus_tag="KRH54_001350"
     CDS             182..1618
                     /locus_tag="KRH54_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG8579017.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESSLVKIFANNFIEIVN"
     gene            complement(1710..3584)
                     /gene="asnB"
                     /locus_tag="KRH54_001351"
     CDS             complement(1710..3584)
                     /gene="asnB"
                     /locus_tag="KRH54_001351"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG8579018.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            3787..4461
                     /locus_tag="KRH54_001352"
     CDS             3787..4461
                     /locus_tag="KRH54_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBG8579019.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLIATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            4574..5110
                     /locus_tag="KRH54_001353"
     CDS             4574..5110
                     /locus_tag="KRH54_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG8579020.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(5276..5896)
                     /locus_tag="KRH54_001354"
     CDS             complement(5276..5896)
                     /locus_tag="KRH54_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBG8579021.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(6210..8348)
                     /gene="vanT"
                     /locus_tag="KRH54_001355"
     CDS             complement(6210..8348)
                     /gene="vanT"
                     /locus_tag="KRH54_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG8579022.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKHLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVNMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINDCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLAE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(8366..9172)
                     /locus_tag="KRH54_001356"
     CDS             complement(8366..9172)
                     /locus_tag="KRH54_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG8579023.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(9169..10269)
                     /gene="vanG"
                     /locus_tag="KRH54_001357"
     CDS             complement(9169..10269)
                     /gene="vanG"
                     /locus_tag="KRH54_001357"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBG8579024.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(10530..11612)
                     /locus_tag="KRH54_001358"
     CDS             complement(10530..11612)
                     /locus_tag="KRH54_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG8579025.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFMNHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            complement(11662..12363)
                     /locus_tag="KRH54_001359"
     CDS             complement(11662..12363)
                     /locus_tag="KRH54_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBG8579026.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(12797..15328)
                     /locus_tag="KRH54_001360"
     CDS             complement(12797..15328)
                     /locus_tag="KRH54_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG8579027.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(15436..16053)
                     /locus_tag="KRH54_001361"
     CDS             complement(15436..16053)
                     /locus_tag="KRH54_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBG8579028.1"
                     /translation="MCKKTYLLVVSILIAGLLTACSGSPTTDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(16414..17298)
                     /locus_tag="KRH54_001362"
     CDS             complement(16414..17298)
                     /locus_tag="KRH54_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG8579029.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLVLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(17489..18739)
                     /locus_tag="KRH54_001363"
     CDS             complement(17489..18739)
                     /locus_tag="KRH54_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8579030.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYENSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVNGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            complement(18961..19725)
                     /locus_tag="KRH54_001364"
     CDS             complement(18961..19725)
                     /locus_tag="KRH54_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8579031.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(19719..20444)
                     /locus_tag="KRH54_001365"
     CDS             complement(19719..20444)
                     /locus_tag="KRH54_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8579032.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(20446..20814)
                     /locus_tag="KRH54_001366"
     CDS             complement(20446..20814)
                     /locus_tag="KRH54_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8579033.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKITLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(20990..22534)
                     /locus_tag="KRH54_001367"
     CDS             complement(20990..22534)
                     /locus_tag="KRH54_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG8579034.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKRNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLIRG"
BASE COUNT         7903 a         3830 c         2383 g         8719 t
ORIGIN      
        1 attaagacct tacctttcat ccctacacct aaaggagagg gcttttcggc aattttattg
       61 taattatata tcatttatat aaacaagtac atattttatt ttgaaactac aaaattattg
      121 tactatattg ttttaagttg ttttataata gattatatca tcttattata ggagaaaaaa
      181 tatgatttgt actattaaac cattaaagat ttcaataaaa aagtctactg aacctctaat
      241 tgaatttata tctgatacta cctataaaaa taagagttct tcatatatta aagaagtcta
      301 ctttacaaga tatataggtg ttgatgattc aattgataaa tacatagaaa aaattaaaaa
      361 tatagatact tattttttta ataatcctac aattccctat attagattaa acaatctaaa
      421 aataaatttt gacaaagaac aaacagataa gatgcttaaa atttttaata tttataatag
      481 ttctaagttt aacagttggg atttatataa tatcgacttt ccatgtaaaa tagaaaatga
      541 caccttaaat tggactaaaa agattgcatt taaaaatact ttagatttat tttccacaag
      601 tatgccaaat tgtacatcct ctatcatcaa aaactttgca gttaaacttt tgtgttggat
      661 agactatttt ctacctaaat tattttataa caaggtgaag actactattt caccaaagat
      721 agtatacttt ggaaatatta aaaggcaaga actttttttc ttatattttt tatcacaact
      781 tgggtgtgat attttatata taaatccaaa taaagatatt cttgatttat atcctgaatg
      841 ttcaaaattt tctactctat ttgaactttc taggaagact ccaaatatat tagaaatacc
      901 ttttgaaagg catattacaa atataaatta cagtgaaaat accaataaga cttctcctac
      961 tacgaataaa cctaactcta aaaatacact taataacaca aatctaacat ctaaaaaaat
     1021 tgtacaagat gaggaagtag aactttctta tgaagacctt gcaaagttat cagcttctgt
     1081 tgttatgatt gttgtgtgca ataacgaaaa taaaccgttt aagagcggtt ctggagtagt
     1141 aataaataat gaaggttata tacttacgaa tcttcatgta gtcaatgatg ggtattcatt
     1201 tttagttaga tttgaaaatg atgataaagt ttatacatct taccaaatta taaaatacca
     1261 ttcagattat gatttagctg tgataaaggt tgatagaaaa tgcaagccaa tacctgttaa
     1321 agtcagtaaa aaacctgtga gaggtcaaaa aatagttgca attggaagcc ctctaggtct
     1381 ttttaataca gtttcagatg gaattatttc agcttttcgt gattttgaaa cggttcaaat
     1441 gatacaattt acagctccaa tctctagtgg tagttcagga ggagctcttt tagatatgtt
     1501 tggaaactta ctaggattga tttccgctgg atatgatgat ggacaaaact taaatttagc
     1561 tgttgaaagt agtcttgtca aaatatttgc caataatttt attgagatag taaactaatc
     1621 aatcaaaatt ctacttatcc tattccataa taatcaagag ggtgaaaaca ttgcatgctt
     1681 ttgccctctg attaatattg aaaaaagtat taatactcga catttttatg ctcaataaat
     1741 aattgatgcc atagacaaaa tgtaaatata gtccatattt ttctcgaatt atctctatga
     1801 cctttaagat gcgtatctaa aagttttatt gcatattttt tatctataaa ttcatctaca
     1861 tttgaattat aaatagtttc ctttacaata tcccctagag aatcttttaa ccaaactctt
     1921 attggagttg gaaatcctaa ttttttcctt tgaacaacat gttctgggac tatttctcta
     1981 aacgcctctc taagtaaaac ttttgtatta ttcttattta ttttttgctc cattcttaaa
     2041 ttttttgcga catcaaatac atctttatat aaaaatggta ctctaagttc aacctgctca
     2101 gctgtagaaa gtttgctaat tttttgaagt atatcgcctt gaagccatgt attcacatct
     2161 atatgttgca ttttacttat ataatcgtaa ttattttttt gtgcatctct atacaaatta
     2221 gacaacaggt attcatatat attcttttcc ttatacttaa aaaagaatct ttttacttcg
     2281 ctgttttcaa aaacttttgc atttcctata tatctttttt ctaatggtgt tgtagctcta
     2341 taaagataat ttcttccttt tatgtttggc ataagttctg aaactcttcc taatatactt
     2401 ttaatataag taggtgaatt gacaactgat ttcatagtac tatactcttt atatatatta
     2461 tatcctccaa ataattcatc tgctccttct ccagacaatg ctactttaac atgtttacct
     2521 gcctctttaa ttaagaaata aagacctact tctgaagggt ctgctatagg gtcatccaaa
     2581 gaataaatta cattcggtaa tgattttata tattcatctt gtgttatatt aatctggata
     2641 ttttctatac ctaacttatc agctgttttc tttgctactt ctaattcact atatcctttg
     2701 acactaaagc ctgcagaaaa agattttata tttggattaa tttgtgaagc cacagtagtt
     2761 ataatagatg aatctattcc accagacaaa aatgttccaa tttctttctc ctcttccata
     2821 tgagttacta ttgaatctac tactatattt cttatatcat cctcattcac atctctattg
     2881 cctaagtgat ttttagtata aaaaaatttt gtactacttc taaactcaag tttattatat
     2941 ctctttaaag atagtatacc attttctacc ctaaaatagt gacctgctgg tatcagcctt
     3001 attccttgta tcatagtatc ttcaggaagt acatattgaa atgaaaaata actttgtaag
     3061 cttctttcat ttatatttaa atttttcata tattcaagta aaaacttata ctctgaggaa
     3121 aaaattattg cattttcctt atttatataa taaagtggtt ttatcccaaa taaatctctt
     3181 gctccaaata tactttcatt ttctctatca tagattataa agttgaacat tcctttaatc
     3241 ttatgtacac aattcttacc aaattctata taagatgcta ataagacttc aatatcatca
     3301 tttgtagtaa aattgtgtcc ttttttaatt aattcttctt ttatttcatg atagttatat
     3361 atatttccat taaaaacaat aacttttcca tctttttcaa atggtttatt tccataaata
     3421 gcatcaatta cattaaatct ttcaaaatca aaagtttttt cctcatcctt atactctatt
     3481 ctactagaaa gtcttgtatt atttacagat tcttttattt taaatttaat atcttctttt
     3541 ttagatatat cacctttata ataaattgaa atattgccgt ccatataatc aaaattcctt
     3601 tctgtccttt agtcttattc tatatttcat agttttgtct ttggctttaa aacttattca
     3661 atttaaaatg aaattgctat taaattctta taaattatct gttaatattt catatttttt
     3721 ttatttctag aaatactata ttaggaggtg atttttatga agaaaaaaat attaatacca
     3781 gttattatgt ctttatttat aatctcacag tgcataactt catttgcttt tacacctgaa
     3841 aataacaaat ttaaggttaa accattacct tatgcatatg atgcacttga accttatata
     3901 gataaagaaa caatgaaact gcatcatgat aagcattatc aagcttatgt tgataaatta
     3961 aatgctgctc ttgaaaaata tcctgagctt tataattatt ctttatgtga attattgcaa
     4021 aatttagatt ctttacctaa agatattgct acaactgtaa gaaataatgc aggtggagct
     4081 tataatcata aattcttttt tgatataatg acaccagaaa aaaccatacc ttctgaatct
     4141 ttaaaagaag ctattgatag agactttggt tcttttgaaa aatttaagca agagttccaa
     4201 aaatctgctt tagatgtctt tggttctggt tgggcttggc ttatagctac taaagatggc
     4261 aaattatcta ttatgactac tccaaatcag gatagccctg taagtaaaaa cctaactcct
     4321 ataataggac ttgatgtttg ggagcatgct tactatttaa aatatcaaaa tagaagaaat
     4381 gaatacattg acaactggtt taatgtagta aattggaatg gtgctttaga aaattacaaa
     4441 aatttaaaat ctcaagatta attaaatatt aaaatccttt tgaaaaaaac ttatgtttgc
     4501 tatataatat aataaaagat agtctaaatt taccaatata atgtttatag tgacgattca
     4561 ggaggatttt tatatgataa aagaaacaca tgaaagggga ggttatatat tcgcactttt
     4621 gatactcccc tttataaaca atctgtacct agttaaatac gatattgcat ataaaatagt
     4681 attaattata atttatattt attttgcata tttaggttct ttatttccag atatagatat
     4741 gagaggttct tacataagta aaaaatttat gttaatatac aaattatttg gaagtaggtt
     4801 tagacataga gggtttactc acagtctaat tgccttatta cttatttata gtttttttaa
     4861 atctctgaca atcttcacta acaataatat agttttctca tgtttgtcca gtgggtttat
     4921 aataggatat ctatctcact tgtgtcttga ccttattact aaagaaggta ttgaattgtt
     4981 ttatcccatt accataaaca tttctttact acctataaaa actaactcaa aaacagaaaa
     5041 attcatatct aagctattaa atttcatagt tatttttttg attggatatc aattttatat
     5101 tttattatag ttgtagaaaa aataaataac aatattttga actaagtata aataagtttt
     5161 atatatttta gtttacaaaa gaactaaaaa ttttgtacct taatacaaaa aataaaacgt
     5221 atcttaaaat aatttagtac taaatcattt taagatacgt tttatttcgt acaagctaaa
     5281 tcattttatc atctttatca ttgtgatgtt ttttatgatg gaaatcataa ttatgatttt
     5341 caagattttt atttactcta tttccaatct tccatgacaa aaacaccaat aatataaatg
     5401 atgctatctt taaaggactt gttataaaac cttctatatc acttgccacg taacttataa
     5461 ctataaacat tacaaatttt cctgccaaca ttgctggcac aaaatctttt aaatcttttt
     5521 taccaaaagc tgatgcgata gtaactaaac aaccaggcat aaatggacag gcatacgcta
     5581 taaataataa cttaaatccc tgtttatcca tccaacttat tgctttttca gttttactat
     5641 ttctcaactt attaaaaagc ttactatcat taaatctgct tattaataag aataatgaag
     5701 ttgtcccaag tcctgagcca atccatgata acaaacatcc aataaataat ccttgtattg
     5761 cagcatttgc tgtaactata gcaacaagtg gtaaggctgg tataaagctt tctataaatg
     5821 cacttaataa gccgattatt gtggctaaca cccaataatt ttgagtcaaa tcaagtagtt
     5881 cttgtatata ctgcatattg cttttcccct tttttaactt taactattat tatatactct
     5941 atatgtttat tatataccaa tctcaaaaaa tgaatctaat attaaaaagt aaataatcta
     6001 tataagttta cagttaaggt ataattattt tattatatat tataacaaaa aaggtgatgt
     6061 ttcaaaataa aaatctgaaa catcaccttt ttataactta aattgtcatg taagttataa
     6121 aaaatatata ttagacataa tattactaaa aatgtctagt cagagtaaaa taaattacac
     6181 agtataacaa atattactat ttaattgagt tacttcttta taacatttaa ccttcttcct
     6241 actctgctca ataattcatt tgatatacta catgagttat ttgcaacttc agaagcagat
     6301 aattcagcta aattatctct tcctattatt attgccgtat taccaacttc tacattaggt
     6361 atatctgtaa tatcaacagc aagttggtcc atgcaaattc gacccactat tgctgctcta
     6421 caatcattta ttattacgta actttttcca caggataaat ttctaggtat accatcggca
     6481 tagccaatag ataatatagc aattacgcta tctctgcttg caataaagac tcttccatat
     6541 ccaaaactat catctttttg tatttttcta ataagaatta cttgtgattt taaggataaa
     6601 acaggtcgta aatcaagttg caatttagtc gtatcatttg gagaacttaa tatcccgtat
     6661 aatgcaattc caattcttac ataattacac ttaagattgg gataattaaa aaaaccataa
     6721 ctactttgaa tatgtacttt aggaattttt ataccttttt ttaacagaaa atcaattaac
     6781 ttataaaaac attcaatttg tttatttgta aagtctacat cttcatcttt cagactatct
     6841 gaaacacaca agtgtgtaaa tatgccacat atatctaagt attttaattt aaatgccttt
     6901 aaaattgacc tcacatcatt aacatcaaaa cccagtctgt gcatacctgt atcaatttta
     6961 atgtgtgatt ttatgttgta tttctgtttg tttaaagaga tagcatgatt aaggtcaata
     7021 acactctgtg taagattgta tttatgaagt tcctttgccc ttattgggtt agtatatcca
     7081 agaatcaata tttctcctag tattccatat cgacgtaaat caatcccctc atctattgtt
     7141 gctactgcaa atgcttttac acctatttgg tttgcacaag tcgctacttc aaaagcacta
     7201 tgaccatagg cattagcttt tacaactgcc atcaattcac aatcttctgg catagctttt
     7261 tgtatcatat ttacattatg ttccaaatta tttaaatcaa tttcaatcca agctctattg
     7321 gtatttacct tatgttctcc tttcaagtct ttttttagat gtttaagaat caatataata
     7381 attatagaag ctataaaaga tgctatactt accactatat aatgaataag gctattgtca
     7441 accaagacag tttgtattcc gaatatttta gaaaacatac gaataacaac aatcatcata
     7501 ggatgtataa tataaataat taatgatgat gttcgcaata acctagctcg agaacctttc
     7561 aaaaatgtta aggctgtaaa taagaaatac atacatggca ataacataat atacatgcta
     7621 tcatgacgct gtacccctag tttatgtaaa aacattccct cacataacat aagtaagaat
     7681 gatataagaa atccaatcaa gctattttta gttgacatat aattagattt attagatatt
     7741 attccaccaa gtatgaaaaa tacaggtgca aaaaatatac catttcgtgt ataatctgat
     7801 acttcaaaaa tatacccata taaattcttt aatagtggta ttttttctaa aaaaccataa
     7861 taactatcac caaacatacc aattacataa ataacaatag tgattactag tgagtacttt
     7921 aaacctagtt tttttacaag aaagtatgca atacttgcac caataattga tgcgggtaga
     7981 taccataaat gatataaagt cccatcaaat acaatgtcct taataatatt gggcactaaa
     8041 ttattcatgg aaaaatatcc agtgtaaata tttaagggaa tataaattac aatagctatc
     8101 ccgtaaataa ataatacatt ttttagaaaa gatttcagtt tattacaatt ataattatat
     8161 gttgaaatta gaaaaaagcc tgatagcata aaaaagaatg gcacagcaat acgagcaatt
     8221 atactagtta acacaaagtc agccatttta ctatatgata ataatggaga tgtatgaatt
     8281 gcaattataa gtaatgcagc tataatccta aaataatcaa gccctgcata agatttattc
     8341 ctaatcattc ttgttcctcc atttcttagt cctttctatt tctccaaaat gtcacaataa
     8401 aaccatcctt attgttaccc gatatttgat atacacattg ttcaggtata gagattctca
     8461 cttcttcttt agtatggata ggcacataaa agatttctat aattttttct ttatcttcta
     8521 tttttaaaca atttgtatac gaaaatctct taatatattc tatatattct tcaagagcga
     8581 gattcatttt attgataatc tcagaatgcg gatatcccac ataacgaaaa tgccatggtt
     8641 cttgagatat tcctgttatt tcttcttttt tacactgata acgctctata aaaccataat
     8701 gtagtgctgc cttacgaaac tcattacaaa taccattata aggaaattca gggcagataa
     8761 aatctataac ttctttcttt aaccctaaat caatagcaag ccctgtctga tgttcactgt
     8821 gatttggaag tgctacaaac tttttagtaa agtcttttcc attgtcacgc agagaacttg
     8881 aatatatatc ttgttgttcc tctgcagtac gatatccact tacaggtact atttcattta
     8941 ttccacatat actttttaaa atattttgta aaatgtttgt agcttcacgt ttcataagaa
     9001 tacttggaaa ccttgtatct acaggaatta aatctatatc ttctgatgtt ttaactggta
     9061 aaaaagcatt tactagaatt agattcccat cataaacttt atctttagtc aataagatag
     9121 tatttgtcat tttatcttct ttatttttat ctaataaaat attattcatc attttatgta
     9181 cacctcaatt atcttgtcca atatttcttc aaaagacagt ccaatacctt tcatcatatt
     9241 tggataacga ctatgagaag taaagccagg aattgtattg acttcattaa aaataatttc
     9301 accagatgga gttaaaaaca tatctactct agcaaatcca gaacatccaa gagttctata
     9361 aataattttt gctgtctctt gtattctttt ctcacaatcc aaatcaattc tagctggcat
     9421 atgaattttt gaacttttca atgtatattt ttctgtataa tcaaaaaatc cttttgacaa
     9481 ttctatttca tctactcttc caacaagtaa gtcatcgttt cctaaaactg cacaccctac
     9541 ctcaaaacca tcaatatttt cctcaattat tacttcatta tcatgttcaa aagccaagtc
     9601 tactgctact tgtaactctt cttttttata aattttacta ataccaaatg atgaacctgc
     9661 acgcacaggt tttataaata atggataagt taagtctttt gacagttcag ccacattaaa
     9721 tttatctgag tattttagta atattgattt tggtacttta ataccttctg aatgaacaag
     9781 tttatgtgat ttgtctttgt ccatacataa tgctgaagaa agagtaccac atccaataac
     9841 ctgtatacct gccaattcaa ataacccctg cactgttcca tcttctccat tttttccatg
     9901 cagtacagga aacacaaaat caagtttagt gtatttaata tcattttcac atgattcaat
     9961 agcactattt ggaatatgat tctgagaaac agcatcagga attaaaaatt caataatacc
    10021 atttttgaca cggttctgcg aaacaataac tggaattata tcagtatccc tttcaaacca
    10081 agtgttattc tggatattat tataatctcc aatataatga taccaattac cctcccttgt
    10141 aattccaatt ggtataatat catatttttc cgtattcaaa ttctcaagta ctgcaaaagc
    10201 tgattgtaat gatacatcat attctgttga gtttccacca aatataactg caattttttt
    10261 attattcatc tttgattctc ctttgttgat ttatttattt ttatacaatc tacagaaaca
    10321 tgttaaaata ctttttctat ttcaaatcca atcaaccttc acttacaatt gcatctgttc
    10381 cacataatat attttcacat ttttctattg atataattac tgtatcaaaa taaaagcact
    10441 cttgttttga tacagtaatt atatcaataa caagagtgct ctttatttat ttatcctaaa
    10501 gaatttccta tttttttata cttaaattct tacaaatttc ctacaatcgg aataataacc
    10561 ttaaaggtaa ttatattgtt ctcgctatga gctgttattg tcccgttatg aagctccact
    10621 atttctttag ctatagcaag tcccaaccca gctctaccac tgtttgaatt acgagatgta
    10681 tccaacctgt aaaattgttc aaaaatacgc tcaagttttt cctctggaat cgtattgcta
    10741 tgattcataa atttaagctt tatataatca tcctcctgta cagctgttat ttcaatagta
    10801 gtatcttcaa aactataata aacagcattt cgtaataagt tatcaaatac acgttgcatt
    10861 ttattaacat cacaccttat catcatattt ggtgtaacat tcagtgtata ttttaaattc
    10921 ttactcatca aagtaggttt aaactcatat gttagttgtt caagcatacg agttaagctt
    10981 actgtactgt attcaagtga tatatttgat aaattaaacc ttgtaatttc aaagaattca
    11041 ttaattaaat cttcaagacg ctctgcctta tccaacgaaa tagataaata tttttttcgc
    11101 agttcttcag aaatctgact ttcatctcga agtagtgtca gataccctat aactgatgta
    11161 agtggtgttt ttaaatcatg tgcaagataa acaataagtt cattcttacg ttgttctgcc
    11221 atttgtgtct caaatttacg ttgttcaagt atatgtttaa ttgagtttat tttcttttct
    11281 attgccaata actctggaga taaagccact tctccaacat cttctttgat aagagagtca
    11341 ataccttgat ttatttcact aaaatatttt gtaaaccaat ttaaataaac acggaatatt
    11401 ataaaaaaca caatcaccat agctagtatc atgaagagct ccatgctatt acggaaaatc
    11461 tgctgatata aattcaatgc agctttatat tccatacgaa aagcattttc aaagaatgat
    11521 actatccaga tagaaaactt accatacaat ataaaagaat ataaaatcca tatgaaaata
    11581 atggctataa atgccattaa agctgttctc ataaaaattt tatttctcac ttgagtaaag
    11641 tcaggattga ctttaatatt tttacttttc aattgtataa cctactcccc atacagtttt
    11701 tataaatttc gggtttttag aaggttcatg aattttttca cgtaatcgcc ctatatgagc
    11761 cataactgtg ttattattat caagatattt ttctccccat acagcttcaa acaattcttc
    11821 agaaggaacc acttttcctt gatgttcaca taaataccaa agaatagaaa attcaatcgg
    11881 tgttaatgct acttctttcc caaataaact acatttatgt gtttctttat tgataatcaa
    11941 tcctttaata tcatactcat taacaattat cgactgttgt tcataagaat tattatatcg
    12001 catatatctt cttaattgtg tctttactct tgcaacaact tccaaagggt taaatggctt
    12061 tgttatataa tcatcagcac ctatggttaa acccattatc ttgtctatat cttcaatttt
    12121 agctgttaac ataatgatag gataatagta tttttctcga attttctggc aaatatgaaa
    12181 tccatcaata tcaggaagca taatatctaa aatagccata tccaaatttt tagattcaat
    12241 acattctaga gcttccttac ctgtataaaa cttgtatact gtaaaacctt cattttttaa
    12301 atatacttca attaaatctg ctatttcttt ttcatcatcc acaatcaaaa tattttcgtt
    12361 catagattat ttttcactct ccttattatc gaggtcatca tttataatat tggtaaattt
    12421 acatatcaaa taattacaag catatttgta actaattttt ataagaaaag aactactaca
    12481 tcagaatgtg ttgatgctac tattggaaca caaaaactga gattaattgt tattataaaa
    12541 actcccctaa cacgtcctta ttatcgcata acaggtatta actttcaata aaaatatcta
    12601 tcagtcaaac acaattcaat ttattatacc agagacaatc catataaaaa acaattaaac
    12661 ctatgtatgt aaccacagac attactaaaa tttccatgtt attttatata aaaattcaaa
    12721 aaaggagttg aatttaaaat ttgattagaa aaaaatcaat tttaagacaa ctccttttta
    12781 ttttaatgta tttaaattat agtatatcag ccttcataat atcctcaaaa tcaccttttt
    12841 cagatattaa tcttatcaat ttaacagtac agcttaaatc atttataagt atagctccaa
    12901 ctaatgaacc atctttcata aataattttt tatatattct ttgaccttca tcttttgatg
    12961 aaatttcttt atcaaaattt tgtatattac caatactaaa tacttttaca ttcattgcat
    13021 ctaatgaaac tggatatact tcactttcaa atgtcaaatt atctccactt gcatttgccc
    13081 ctgctatttt tcccatttct actgaagatg gccatattgc taaactaata tttcccactt
    13141 gtgcaacatc tccacaagca tatatatctt taacatttgt ctgtaatgtc ttatctacaa
    13201 caatacctct atcaattttt acagatgtat cctgaaccat ttgagtgttt gctcttacac
    13261 caattgaaaa tactaccata tcacagtcga cactatctcc atctttaaat acaacttttt
    13321 gagcttttcc atcaccttca ataccatcaa ctgctacacc caatcttaca tctattcctg
    13381 tatctcttac acattgttct aatattttac taccttcttt atcaagttgt tttgatagta
    13441 tgctatccat ggcttcaact actactactt ctaatccttt tagtctaaat tcccatgcag
    13501 cttcaagtcc caataaaccg ccaccaatca ctacaacttt cttagatttt tctagtgctt
    13561 cttttacact atataaatct tttatatttc taagagtaaa cacattttct aaatcatggc
    13621 ccttaattgg tggtataaag tttctacttc ctgtagctat tacaagctta tcaaatttaa
    13681 tagctccatc attcactata atagttttat tcacttcatc taatttatct acattcgttc
    13741 ctaatattac tactatatta tttttatcat accaatcttt atcttccatg tagaaatctg
    13801 aaccaagttc ttcatttata ccatcagaaa gtgcaggtct atagtaagtc aactcatctt
    13861 cattagaaat catagttatc ttacaagttt tatttctttt tcttatagca tctgctgcat
    13921 aaaaaccagc tgctccatta cctactatta cgaaatattc atcagtatct tttctaaaag
    13981 taacttcatc ttcttcaact tctataaatt ggtcttcctt agctccacat acagggcata
    14041 cactaggagc actatcacct tcaaaaactt cattacaaat tacacattta aatttcttag
    14101 ttttcttttt acttccaaaa gtagaattta acttatctag gaatttacct gtataagaat
    14161 gtaatttatc aatctcttca tttgatggtt taaagttaac agcaaaaggt tttactgctg
    14221 tcattctaag ctgacttatt ctctccatag cattttctat accttctcca ctccatccat
    14281 atgaaccaaa tactgaagca acttttccac catgaagaac tgggtttaac gatattaata
    14341 tatcccatat aggctttaac gcatctccta atattgttgg tgttccaaat aatacacctt
    14401 gtgatactgc tatttttgcc aaaatatctt cttgcttatg ctctattaca tcaaataaat
    14461 ttactttata gttgctattt ttttcaatat atgcttttat agcttctgcc ataatctttg
    14521 tatatccatg tgcagaaaca taacaaattg taacttcttt ttctaacttt atttgttcat
    14581 ttacactcca gttgtaataa aggtctataa tttttctagg attttcagtt aatactggac
    14641 catgacctgg acatacagta tctatatcta aatcttttat cttttctata gcagttacca
    14701 tagatggttt aaatggaccc attatacaat cataatagta tcttaaagca tccaagtaat
    14761 ccttttcttc attttcatca agtgtattta ctatcttatc attactatag tgactaccaa
    14821 atgaatcaca tgttacaaga gttttatcct cttttatata tgtgtatatg gtatctggcc
    14881 aatgaagcat aggtacagag aaaaattcta aagttttgtc cccaattgat aatgtatctc
    14941 catctgtaac tgcaacatat tcaaagtctt tgtttacgat ttccttaaga tatttaattg
    15001 ctgtttctga agctactact tttgcatttt tagctaaatc caaaagcttt tcaacacttc
    15061 ctgcatggtc tggttcagta tgacttacaa caatatagtc tattttttca aaatctatat
    15121 ttaaatcatt taatctttca atataattgt caaaatgctt atctttaact gtctcaaata
    15181 aaactgtttt ttctgttcct tttaatacat atgaattata agtagtccca taaggtgtat
    15241 acataattat gtcaaatact cttaaatcag ggtcaagtga acctacccaa tataaatttt
    15301 cttttatttt tagtgatttc aatgacatac cgtactcctc ctaaaatagt tatttagtct
    15361 ctttataccc tgcttgttat attttaaaca aaatttattc aaaaaagaaa ttattttaaa
    15421 gatagtttaa atcttctaat cttctttttc taccttcaat acagttccct ttttagaatc
    15481 tacttttatg tcatattcat ttttatcttt atctattctt attttataaa aagtaacatc
    15541 taaatctttg tctaaactcc attctttaac tattccattt tgttctttta atgcaatttc
    15601 catagcttgt tgaggtgtca ttatgtcatt taaatctaat ttttcttttc tagcaactcc
    15661 atttaaatct tctgaatcca acttttctgt tttatcttct aatacatctt ttgtattagc
    15721 atcaattttc atcttatatt cattattatc atctacacct tccacagtat aaacaaattt
    15781 atttaaatct ctctcaagac ttaaatctac tatatcagca tccttatatt tatctttaaa
    15841 tatgtttatt gcatctgtgt atggcacttt aaaatcttgt tccattaaat tttcattatt
    15901 tttttcatct tttaaagcgt tatttgatgc atttgcctcc ttttttgtat tatcttgtgc
    15961 ttgtcctgaa tccgtagttg gactcccact acatgcagtt aataaacctg ctataagtat
    16021 acttaccact aataaatacg tttttttaca cataaaaatc ctccttaaat atattaattt
    16081 attctatatt atgccctctg aaccctttat ttaccctttt atattaaaaa tttctcctat
    16141 tttatcaatt ttaaaacttt atatttacta aaattaaagt tacataaaaa ttatactgtc
    16201 tcaaaataga aacaagcatc tttttcattg ttgaaataat aaaaatatat tttatttcgt
    16261 acaaaaagag tgcctcatga actaaaaagt tcattttgag acacgcccat tttttatatt
    16321 tttacttaca aaataacagt acttattaat tttttacaaa ccttataagt atatctatat
    16381 gatattctta aatttaaaca ctatattaaa aaattaaatt ttgtttaatt cttttactgt
    16441 taaatccatt ttaggatatg gaatcgtaat tccttcttcg tcaaacctaa ctttaacctc
    16501 ttctagtaaa tcaagataaa ttgtccaata atcttctgtc ttgcaccaaa ctctagttgc
    16561 aaatttaata gcattatctc catgttctac tactcctata aatggttctg gttcatttat
    16621 tattaattta tgattattaa caatatcttt aagtactctt ctaacatgta gtatatcatc
    16681 ttcataccct acactaaaag tcaaatcaac tcgtctagta ttctttgctg aatagtttat
    16741 tagactatca tttgatacag aaccattagg tataagtatc tgtttattat ctggagtaac
    16801 caattgtgta taaaaaagtc ctatttgttc aaccgttcct ccatggcctg ctgcttctac
    16861 gtaatctcct actttaaaag gtctaatcaa tagtataata aatcctccag caaaatttga
    16921 tagactacct tgtaaagcca aacctattgc tacccctgct gatgctatca cagcagcaaa
    16981 actggacaaa ctcattcccc aatattccag aacaatgatt ataacggcaa ctttaagtac
    17041 actcaaaaga agagacttca aaaaccttct taaagttaaa tcaatatctc ttttactaag
    17101 tactaacata acatggttta caaatttttt tattatttta aatccaatag aaagtataag
    17161 tatacctatt ataagtttta ctccgcttgt agtagcccat tctagcagac tttccattat
    17221 acctcccata tccatcttat taatgtctaa attatttaaa tctaacttgc ttatatcatc
    17281 tattttcgtc gtatccataa aaaactcctt accttatatt ttagaaccaa tattatagtt
    17341 taaagttctt attttctatt taatataata caataccatt tgtacatagt aaacaagatt
    17401 tatattccaa ttatacatct taggaaaatg caaactattt gttatatata tgcaaaatat
    17461 aatactaaat taaactatta ataaaacact atatagcctt aaaatcatca ttttttaccc
    17521 ctatatgaga tgatactata gttataatac ttagtactat aaatactaca aaaccttttt
    17581 tatatccatt aactatatta tatgaattta cttgtaaaga gatagctata atcaaattaa
    17641 taattccact tcctataaat tcaaaagtat taataacact agttgccagt ccagaataaa
    17701 tttctttatt tttatacttg gcatcattaa aaacctgtag atgtgacata actatacatc
    17761 ccataataaa gaatgatatt ggtaaaacta taattggtgg ttttacctgg tatacaacta
    17821 caattacaat ccatataaac aaatctatca ttgccccaaa ttttattata ttaaatttgc
    17881 tagaccttat tttagcaaat acaaaatcca taattattga accaaatata aatccatatg
    17941 taaaaaagga tactataaac gcagaaaaac tcttactcac accataaacg tccattatgt
    18001 atcttactcc ccacaagctt ataaatgcac ttgtaagacc cacaaaagag aacattatca
    18061 ttgaattata ccatgttgat ttgttcttta taacagactt aataccatca acaatattaa
    18121 ccttctctga attttcatag ggttcagtat ctacattgaa tccatactct tttggtgtat
    18181 ctctaacaat aatatacata aaacatccaa ttacaacacc aattataccg attaataaaa
    18241 atgaatttct ccaaccaaca agttcagaca aaaacacaag tggaaaagta gcaagaactc
    18301 cacctaaatt tcctacaaat gaaaatttag ctgttgcaga agcaaattct gacttattaa
    18361 accatctccc ttgaattttt aaaatacaaa gtaatattac tgatgttcct gcccctacaa
    18421 ttacacgaga aatatatgct aattctatac tttgaatcaa accaaataaa attgacccta
    18481 aacttgccat tattattcca agactgctaa tttttcttgc tccatattta tcaatcaaaa
    18541 ttcctgcagg tatttgcata aatgcatatg catataggca aaaggatgct atatttgaaa
    18601 tttgtataga attaaaacct aactcatttg ctaaattgtc tgaaacaaca gcagttgaca
    18661 tccttaaaaa taatactaaa acaaatgaaa atgtaattat accccaaaca ctccacttag
    18721 cctttgtata acttttcata tcttctccct caaatccata tatttttaaa ttttcttgat
    18781 ataacttata taaagtgtat agttattctt acaaattttt acacgcaata tcattataca
    18841 acatatgtat atatttaact agaaataaaa cactattcat attatataca tgaattaact
    18901 acataaataa taagagccat cttttaacat tactcaaaag atggctccat ataaaaacat
    18961 ttattctaaa tttaattttt tatctaatat aaattttgta gcgaaaaata acgctattgc
    19021 aactacaaga ctaccaagta ttgcaagcat aaaactagga ctctgataaa gatgatatac
    19081 catatttgta tcttcatttg gtaagatgct tccaactgca tctccaacaa ttgatattac
    19141 tatatttata gctataaaag aagcaaatgc cactatgttt ctatgtttat tgaattttgg
    19201 taattgtccc attgagattg atgtatataa taacaaaata aatgatgaat atgaaataaa
    19261 cattactact actaataata aaatatctaa aaagttttca gaaatccatt taaaactagt
    19321 gttaaacaaa tctaaaattt caggtagtaa aattccagaa gtaccaaaaa gcataagaac
    19381 tccaaaagtg aatactgcaa ctataaaact aagtacagcg tatataagtg ctgttataca
    19441 tttagataat ataagtgaac tagtgctaac aggaagagta aacattaaat aaccttcatc
    19501 ccccaaaaga ttttttctaa acctttgaat tgtaactaca atagttaaaa ctcctaatgc
    19561 cataaaaaga cttgttaata ctagtattcc tattccctgt acctgaagta tatttgtatt
    19621 cataaatatt ccattaaata cagctactat caatatggct atatataacg gtataaatat
    19681 tcttccactt gcttttaact catattttaa taatttacct aacatttaaa ttcctcccta
    19741 aataatgcat ctatagattt tcctttttct tctcttattg cttcaacgtc tccttgtaac
    19801 actatttctc cttttgatat gaatattatc tcatcacata tgttttctat ttcacttatt
    19861 aagtgggtag caatcagtaa agtactatct tcagaataat tttttaatat agtcttaagt
    19921 atatatgacc ttgcagctgg gtcaacacca cctataggtt catccaatat atatatgctc
    19981 gcatttcttg acattactaa aataagttga actttttctt tagttccttt agacattgtc
    20041 tttagctttt catttacatc aatatctaag cttttaatca tctcatttgc tcttctaaca
    20101 tcaaaatcac tataaaaatc atagaaaaac ttaagtaaat ctgatacctt catccaatca
    20161 tttaaataag ttctttctgg taaatatgaa actatttttt tagtatctat ggatggtttc
    20221 attccattta tcataatttc accattgtca ggctgtaata atccattcat tagcttaatc
    20281 attgtacttt tgccacttcc attaggtcct aacagcccga ctatttttcc ctttggtata
    20341 tttaaatcaa tattttttaa aacattctta gttccataac tcttgtttac acctttaaat
    20401 tcaactattc catcttgaga aacattgctt atatttttat ccattttata attctccttt
    20461 cagattttct gtaataagtt ttagcatttc atctctatca tatccaagct tttctaaggt
    20521 tatttttaat gtatttatct ccacattagc tatttcctct ttcatagctt ttattttttc
    20581 cttatcatct gttacaaatc ttcctttagt tctctgacta taaactaaac cttgtctttc
    20641 aagttctgtt agagcctttt gcattgtatt tggattaact tccgcatctt ctgctagtgc
    20701 tctcactgtt tctaatttag atcctatttt aatttcccct gaaattattt ttaactttaa
    20761 atgttctact aattgtatat atataggttt gttattatca agttcccatt ccatgttcat
    20821 gcctccttat tatattattg tattaagttg ttaatacaat aatataataa tacaaagtat
    20881 atgtcaatta ctttttagta aaagtataaa aaattttaca aaaaaaatga acactacatt
    20941 agagtccatt tttaacacaa tttattaaat tctttatatt acagattaat taccctctta
    21001 tcaagttgga tacaattaaa tgttcaaatt cttcaattgg cattggcttt ccaaataaat
    21061 atccttgtgc catatcacac tttacttctt ttaaaagttt aaactgttct tctgtctcaa
    21121 ctccttctga tatgactttc atacccaact cttttgccat ttctattact ttagtaacaa
    21181 ttattttgct tcttttacta ttataagttt ccataataaa tttttggtca agttttaaga
    21241 tatccatagg aatttctttt aacatattta atgatgagta ccctgaacca aaatcatcca
    21301 tagatattaa aaatcctatt ttttttaaat tattcattat atcaattaaa atatcaaaat
    21361 tatcaaatat gatactctcc gttaattcaa gttctatata ctttgcaggt atattgtatt
    21421 tatcaattat atttttgtat gtttctataa aattattgca atataaatgt atccttgaaa
    21481 cattaactga aacagtaact ggatttattc ctgcatcaat ccattttcta aggtatatac
    21541 atacttcttc aagcacatat aaatctaaat ttataacaaa accgtttctt tcaaaaaatg
    21601 ggataaattc atttggaggt attagccctt tatcaggacg tttccaccta acaagtgctt
    21661 ctgaaccttg tatctctcct gtattaagtt ctatcttagg ttgtaagtat ataataaatt
    21721 ctttattttc tagacttgaa aacattgtat tttccatctc tttttctttt atctcttggt
    21781 catgaatctt attatcataa aatgcatatg aacttttatg tcctcccttt gccattttat
    21841 gaggtatatt agccctatct atgattgagt tgatatctct atcttctgga agtataaaat
    21901 agattccaca acttaaaact aacttatatt tttcttcttg attattatta aacaagtcta
    21961 attcttcaaa tatagttttt aacctctttg ttatatcttc ttcttctata tatttcataa
    22021 ttataataaa attatccgca gatattcttg caaacatttc ctcttcaaat atattcctct
    22081 taataatctt tgttaaatta cgcaaaattt cattcccaac ttcatatcca aataaatcat
    22141 ttatatattt aaatttatca atatctatat ataataaagc atactttact tgagtatttt
    22201 tacatagtat tttgttagca tcaattacaa atttatctat agtatttgct cctgtaagat
    22261 tatctgtata tgctatcctt tgcaatttaa ttttaaaatt taatttatca tatattatat
    22321 agaatataag tattacacat attatagcca taataagtat tatttgtatt gcatgtttgc
    22381 taataagctg attattagac acaatagact caaagttatt tatggagttg tttttataga
    22441 gtttgcataa agaatagact ggtgttgtgt atgtaatttg taaaattata caaataaata
    22501 aaaaaaacat caattttttt tgtaaattcc tcataaaaat ttcctcccat taaaacacct
    22561 ctattttatt agcaaataag tacatctcaa aagtttccat ttcttgtaaa atggatagtt
    22621 ttgaaaagaa aatattaaaa ttataatata aaaataaaat ttttcagaca atttaaacaa
    22681 aaataatcac cttttaataa ataattatat gccaaaataa tccaatttat tttctattat
    22741 acctatatga aatatataaa tcaatagtta agacaatgaa tatactacat tctataatta
    22801 tagtagcaga aaattttaca aaattaaagt agata
//
LOCUS       DAEOSP010000043        22812 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.043, whole genome shotgun sequence.
ACCESSION   DAEOSP010000043 DAEOSP010000000
VERSION     DAEOSP010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22812
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.043"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..1110
                     /locus_tag="KRH54_001368"
     CDS             <1..1110
                     /locus_tag="KRH54_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG8579035.1"
                     /translation="VKYIIKQSTVLSIVSTLLVYLIEQLVYGFTAPLNYLIFTSDINT
                     DIYKFDINMGLSSLITIIIAGFIYWYSSKKFNIKVMNFDKYIAILMIPLLLIILFMQS
                     FEYSSNINIDTSLGIVKLLLNTSITEEIQAFLFSIIGTICVFFSLFTFKKLIQALEDD
                     KERAIMNQQIHAQKNYIEEAKSRLSQTISFRHDFNNHLAIVNGLLKKDQILKAQDYLN
                     KLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQLGIEVDCEVIIPQSTNMNDFDLCIV
                     FANAIDNAIKACRVVDKQNRYLKLSTKLEGGFFMIEIKNSYNPINNYTSGSGIGLLNI
                     KEIAKKYQGAISIDKTSDYFQINVLLVISLHCASI"
     gene            1149..1634
                     /locus_tag="KRH54_001369"
     CDS             1149..1634
                     /locus_tag="KRH54_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579036.1"
                     /translation="MYLNRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKSLQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKAKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(1897..2133)
                     /locus_tag="KRH54_001370"
     CDS             complement(1897..2133)
                     /locus_tag="KRH54_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579037.1"
                     /translation="MLLTHIYFNGQFKQTIELYKQAFNATVKTLIEDSCYLLIVHNEI
                     VIHNKFLILNDFGNKNGVSISEGYQPTDKIQTAS"
     gene            complement(2288..2512)
                     /locus_tag="KRH54_001371"
     CDS             complement(2288..2512)
                     /locus_tag="KRH54_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG8579038.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(2609..3862)
                     /locus_tag="KRH54_001372"
     CDS             complement(2609..3862)
                     /locus_tag="KRH54_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBG8579039.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            4122..5111
                     /locus_tag="KRH54_001373"
     CDS             4122..5111
                     /locus_tag="KRH54_001373"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBG8579040.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            5183..5923
                     /locus_tag="KRH54_001374"
     CDS             5183..5923
                     /locus_tag="KRH54_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBG8579041.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            6139..7290
                     /locus_tag="KRH54_001375"
     CDS             6139..7290
                     /locus_tag="KRH54_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG8579042.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            7315..8070
                     /locus_tag="KRH54_001376"
     CDS             7315..8070
                     /locus_tag="KRH54_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBG8579043.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            8262..10649
                     /locus_tag="KRH54_001377"
     CDS             8262..10649
                     /locus_tag="KRH54_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBG8579044.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGCTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(10880..11113)
                     /locus_tag="KRH54_001378"
     CDS             complement(10880..11113)
                     /locus_tag="KRH54_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBG8579045.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            11402..11992
                     /locus_tag="KRH54_001379"
     CDS             11402..11992
                     /locus_tag="KRH54_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8579046.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEYFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            12106..12951
                     /locus_tag="KRH54_001380"
     CDS             12106..12951
                     /locus_tag="KRH54_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8579047.1"
                     /translation="MIKVDDLSFSYTDRDFLQNINFEVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENESNKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            12948..13631
                     /locus_tag="KRH54_001381"
     CDS             12948..13631
                     /locus_tag="KRH54_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8579048.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLIIASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            13628..14344
                     /locus_tag="KRH54_001382"
     CDS             13628..14344
                     /locus_tag="KRH54_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8579049.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISLLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            14441..14704
                     /locus_tag="KRH54_001383"
     CDS             14441..14704
                     /locus_tag="KRH54_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8579050.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            14676..15131
                     /gene="def"
                     /locus_tag="KRH54_001384"
     CDS             14676..15131
                     /gene="def"
                     /locus_tag="KRH54_001384"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG8579051.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIEQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            15183..15644
                     /locus_tag="KRH54_001385"
     CDS             15183..15644
                     /locus_tag="KRH54_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBG8579052.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            15694..16596
                     /locus_tag="KRH54_001386"
     CDS             15694..16596
                     /locus_tag="KRH54_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG8579053.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            complement(16780..17997)
                     /locus_tag="KRH54_001387"
     CDS             complement(16780..17997)
                     /locus_tag="KRH54_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG8579054.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(18079..18282)
                     /locus_tag="KRH54_001388"
     CDS             complement(18079..18282)
                     /locus_tag="KRH54_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG8579055.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            complement(18743..18973)
                     /locus_tag="KRH54_001389"
     CDS             complement(18743..18973)
                     /locus_tag="KRH54_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8579056.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            complement(18970..19392)
                     /locus_tag="KRH54_001390"
     CDS             complement(18970..19392)
                     /locus_tag="KRH54_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG8579057.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            19897..20250
                     /locus_tag="KRH54_001391"
     CDS             19897..20250
                     /locus_tag="KRH54_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8579058.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            complement(20310..20477)
                     /locus_tag="KRH54_001392"
     CDS             complement(20310..20477)
                     /locus_tag="KRH54_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBG8579059.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(20596..22515)
                     /gene="tet(M)"
                     /locus_tag="KRH54_001393"
     CDS             complement(20596..22515)
                     /gene="tet(M)"
                     /locus_tag="KRH54_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBG8579060.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            complement(22531..22647)
                     /locus_tag="KRH54_001394"
     CDS             complement(22531..22647)
                     /locus_tag="KRH54_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBG8579061.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         8434 a         2981 c         3689 g         7708 t
ORIGIN      
        1 gtaaagtaca taataaaaca atctactgtt ttatctatag tatcaacttt gttagtatat
       61 ttaattgaac aattagtata tggatttaca gctccattaa attacttgat atttactagt
      121 gatataaata cagatattta taaatttgat ataaatatgg gactatcttc tttaattact
      181 attataatag ctggatttat atattggtat agttctaaaa agtttaatat taaggtcatg
      241 aactttgata aatatatagc aattttgatg attccattgt tgcttataat attatttatg
      301 caatcatttg aatatagttc aaatataaat atagatacaa gtcttgggat tgtaaagctg
      361 ctattaaata cttctataac tgaagaaatt caagcttttc ttttttctat aattggaact
      421 atatgtgtat ttttttctct atttaccttt aagaaactaa ttcaagctct cgaagatgac
      481 aaggagaggg caattatgaa tcaacagatt catgctcaaa aaaactatat tgaagaagcg
      541 aaatcacgat tatcacaaac tatatccttt agacatgatt ttaataatca tttagctatt
      601 gtcaatggat tgttgaaaaa agaccaaatt ttaaaagcac aagattattt aaataagctt
      661 gaaaaagtaa caagtgacct atcattttct tgtaatacac agaatattgt tattgatgct
      721 cttttcaaca ataaattaag tatagcaaaa caattaggca tagaggtaga ttgtgaagta
      781 attatacccc aaagcactaa catgaacgat tttgacctat gtatagtgtt tgctaatgct
      841 attgacaatg caattaaagc ttgtagagtt gtagataaac aaaataggta tttaaagctt
      901 tccactaagt tagagggtgg attttttatg atagaaataa aaaatagcta taatccaata
      961 aataattata caagtggctc aggtataggt cttttaaata taaaagaaat tgctaaaaaa
     1021 tatcaagggg ctataagtat agataagaca tcagattatt ttcaaataaa tgtattgctg
     1081 gttatttcat tacattgcgc tagtatttga taacaatttc attgaaaata gtatttgtaa
     1141 gctatattat gtacttgaat agagaaagtt tgatatatag aaatgaggag gttactaaaa
     1201 tgaaagttgg aaatataaat ggtcaagcac caattacaaa tggtttagat agttgtcaaa
     1261 gtgaggtaca aggcttagaa caaaagaaaa aatcattaca ggatgaaata gagaaagtaa
     1321 gaagtgataa aagtattgat ggtgaaacag ccaaattaaa aatagaagct ttaaaaaaag
     1381 aaatggaaga gattgaaaat aaaattcaag agttaaaagg aaataaagca aaaaaaacaa
     1441 ataaagaaga aaataatgtt tcagaagtaa ataataatct attagagaga aaaaaagaac
     1501 cttctaataa tgaaaatgta aaacaattat atgatgtttt tgaaagaagc aatgtagacg
     1561 aacaatggga taatacttat cgtgttctcc aagatgataa aaacttaaaa gtaaaattta
     1621 atagacctat atagtttatt tataaaaata gttaatattt taattatcac cactttttag
     1681 aagaatacac actttatttt aaagacccac atatttattt acagttagca catttatatg
     1741 tggttttata actattattt acaagtctat attgataact tttacatagt atataatgtt
     1801 taaatactta ttcaaattta tctatacata aacaaaaaaa gagattttaa aatctctttt
     1861 tagattgttg acttatggct ttttgattta ataaaatcaa gaagccgttt gaattttatc
     1921 tgttggctga tacccttctg atattgaaac accatttttg tttccaaaat cgtttaaaat
     1981 taagaactta ttatgaatga ctatttcatt atgaacaata agcagataac atgaatcttc
     2041 aatcaatgtt ttaacagtag cattaaatgc ttgcttatac agttctatcg tttgtttgaa
     2101 ctgtccatta aagtaaatat gagtcaataa cataactata tctacacaaa ttaatataaa
     2161 ttttttatac tgtacattat ctaactgttg attatctgta ttacaacatg attgaattta
     2221 aaaagccatc aacacataga atgttgatgg cctgaaaata gatttaaaat ctctttttta
     2281 ttatatatta attatttttt aaatttatat tttttgcatc agattttcta atagctaagt
     2341 caaaccctct aaatctaatc actatagggt cattaagagg agctatcttt ttcacttcaa
     2401 cttcagtgcc atctattagt cctaaggcta gaagtctctt cataagtttt tcatttccag
     2461 ctatattgtc tataattccc ttttgtccaa gttttaagtt gtaaacagtc attattaaca
     2521 cctcctaatg aaaatcatta tcaaacttta tatttataat atataacaaa aagagcaaat
     2581 tgtcaatata taacaaaaaa taaacatact aagttagtat gtttataaaa aatagtatac
     2641 atgggatact aatagcatct agcaaaaaac cacttactaa tggtacaact aaaaaggctt
     2701 ttggagaatc accatatttt tgtgtcaaag aactcatatt tgccagagcg tttggagtag
     2761 ctcctattcc atgtccaatc aaaccactta ttatgatggc agaatcaaaa tcttttccaa
     2821 gtactctaaa gcaaataaat attgaaaata ggattataaa gattacttga aaagttacta
     2881 ttaagagaat aggaggtaat aatgtagata attcaaataa atctatattc ataagtgata
     2941 aagttaaaaa aattcccaat gaaagttctt ttaaaaaatc tataagttta aaatttaatt
     3001 ctagtatatt tatcttatca tttaaatttc taaaaataac agccgaaaac atacaagtta
     3061 caacagaggg taatagtata ttaaacgtta tatagaagca tctacttata atttctcctg
     3121 tatttataca aataaatatg agaagaacct gttccaaaaa tacagatggt gttattgttt
     3181 cggagaagtt attatttttt atcctattct tatttctaaa aaatttactt ccaaatctct
     3241 ttagatttct attatatcta ggaatcgaca agtctatatt tgatgatggt tttaatttat
     3301 atttatttat tagaaattta gctacaggac atcccaatat accacccata ataagtccta
     3361 ttgttgcagc agataatcca accccaactg ctccttctat accgagattt tctattgtaa
     3421 gtccaaatgc agcagaataa ccatgaccac cttccataga tacatttcca cacataagcc
     3481 ctaaaagagg ttcaagtttt ataatctttg atattgtaat agctaatata ttttgacaaa
     3541 atgccaatac tccacacaaa atccaatatc taaataatac actaccacct ttctttataa
     3601 gatttatgct tatttcaagt cctatagttg taaaaaagaa ggttatgaaa tttggcatta
     3661 aatttccact tatagatata tctgctatat taaaatattt aaaaaataaa tttagaaaac
     3721 aaaataatag acctcctatt actggtgcag gtatacagaa attatttaaa aagttaactt
     3781 tattcttcaa taaattacca attaaaaaga agaatataga tagtgtcaag gtttgcacta
     3841 tatcaaaact taaggtaatc aaaataagtc ctcctttgta attcaaagtt ttaattatat
     3901 aacttaaatt cttatgtatt gaaattttat tatcaaatgt atattaataa atgtatatta
     3961 aaaagaatca gtagatataa ttagatagtc ataaattaaa ttaaaatatt ttaatgagat
     4021 atgacttttt tagaattaaa aaccgtagta tattgatata atagaaataa gacttaaaaa
     4081 tctgttgata gtattattta taagtacagt aggaggataa aatgtttgaa aatttaccta
     4141 agatagattt acattgccac ttagatggaa gtgttagagt agaaaccatg cttgatatag
     4201 ctattaaaga gaaaatagat ttaccttcaa ataatatgga tgaaataaaa aaattagcaa
     4261 aggtttcttt taattgtact tcactagatg aatatttaga aaaatttgat ttacccttaa
     4321 aagtaatgca atctaaggaa aatttaaaaa gaattacttt tgaacttttg gaagatgcaa
     4381 gtagagaaaa tgtaaaatat attgaaatta gatttgcacc attacttcac acccaaaaag
     4441 gtatgagtgt taaaaatata attgagggta taattgaagg aattagagaa gctgaaagta
     4501 tttatgatat aaaaggcaat ttaatattag gttgtatgag aacaatgacc tcaaaagagg
     4561 cattattagt aattgaagaa gggaaatcat ttgtaaataa aggtgttgta gcagtagact
     4621 tgtgtggtcc agagaaagag ggattttgta aggaatataa agatgttttt aaattagcaa
     4681 gagaatatgg atataaagtt acaatacatg caggagaagc tgcaagtgga gaaaatgtat
     4741 tggatgctat aaatatttta aaggcggata ggattggtca tggggtaaaa attaaagacc
     4801 ataaaaaggc gtataattta gttaaagata aaaaaatatt gcttgaatta tgtccaacta
     4861 gtaatgtaca aactaagaca gtagattctt atgaagcaca tccattttat actttttata
     4921 aagataattt acatgtaagt ataaatactg ataatagaac tgtatcagat ataaatttaa
     4981 gttcagaact aaatgttata tttgatactt ttaaactagg attagaagac tataaaataa
     5041 tttatagaaa tgcagtagaa gctagttttg cagataaaga aactaaagag tatttgaata
     5101 gtttaattta atagataaaa atatttaaac tttaaattaa gctattaaat tgataaaaat
     5161 acattgtatt aggagaaaaa atgtgaaaga ttatgaagat gttgtttata ttaaactagt
     5221 caaaaaagaa cttgaagaat ggaagaaaaa tatattaaag aaaccttcta tatttgacaa
     5281 agcatcaaag ggtatacaga ataaatttaa tggagtattg ccagataaat atcatgaaat
     5341 aattacttct tctataaaag taatgacaaa aaccgtactg tttggatata aatatgctac
     5401 aaagccacca cttagaaata tgtcattaga agaaagagaa aaattagttt tagaaaaagc
     5461 taaaacttac aagactacag caatgattga aggggcagga acaggggcag gaggaatcct
     5521 cttaggttta tctgattttc ctttgctttt aggtattaaa gtgaaatttt tatatgatgt
     5581 agcttcaata tatggatatg atgttaaaga ttatagagaa aggatttata tattaaatat
     5641 attacagttg gctttttcta gtcaacagca tataaacaaa acttttaaat tgatgaataa
     5701 ctgggatgaa tatgtaaata gtctaccaga agacatcaat gattttgatt ggagaacatt
     5761 tcaacaagag tatagggatt atttagattt agctaagttt ttacaactaa tgcctggaat
     5821 aggagcattt gtaggatttt atgtgaatgg aaaattaatt gataaattat gtgaaactgc
     5881 tgtgtctgct tatagattga ggattcgaga gcttaagtta taagaaaaat tataacataa
     5941 aaaattagtt atacaatata tctatatgta tctaattttg cattgataaa aacaattatg
     6001 gataaaaggt aaagcatgat agaatctaat gtagaggttt gtattttaat ggaaaaattt
     6061 agtttttatt gcttaagata caaattgaat tacgaagaga ttgaattatg aagagaagta
     6121 attgggagga atattttcat gacaaatttg ccaaaacaat ttgatagctt caatgaagct
     6181 agacaaaaag gatttataaa tgcaaaggaa cttaaggaaa gtgggaaaaa gatggttgga
     6241 gttttctgta catttactcc agttgaaatt ccaatggcag ctggagcaac tgtagtaggt
     6301 gtatgcggcg ttagtgaaga gccaatacct gatgcagaaa gggtgcttcc aagaaatctt
     6361 tgtcctttaa ttaaatcaag ctatggacat gcaataacag atacctgtcc ttatttctat
     6421 ttttctgatt tgttgatagg agaaaccact tgtgatggta agaaaaaaat gtatgaagaa
     6481 cttgcaaaag ttaagccaac atatgtaatg catttgccaa atacttcaaa aggagaattt
     6541 gcctataaac tttggaaaga tgaaatgata agattaaaag aagaggttga gaaatcttta
     6601 ggtgtaacta taactgaaga agatataaga actgctataa aagataaaaa tgaagaaaga
     6661 gaacttttaa aagagtttta tgcacttggt aaattacaac catctgcact tacaggcctt
     6721 gagttacata atgttttata tcaagcaggt tttaaatttg accgtgcaga acttaaacat
     6781 tctttaagaa aagttataga tgatatgaaa gaaagatatg aaaaaggtga atgtcctgtt
     6841 caaaaagata agccaagaat attgataaca ggttctccta ttggtggaat aagcgaaaaa
     6901 atagttaaaa ctttagaaga tgcaggggca tcagtcgttg catatgaact ttgtggagct
     6961 attagatcaa acgatttatt ggttgatgaa gaaatagaag atgtatatga tgctttaaca
     7021 caaaaatata taaatatagg ttgttcttgt atgatgaata atgacaatcg tattgaatta
     7081 ttagatagaa taatagatga atataatgta gatgctgtaa tagatgtagt tttacaagca
     7141 tgtcatacat ttaatataga aagttataga ataagagaat ttgttacaaa agaaaaaaat
     7201 aagccattta tgtctcttga aactgattat tcaaaatcag atactgaaca attaagaact
     7261 agatttgaag cgtttgtaga gatgttgtag gaataaataa aagtaggagg aactatgttt
     7321 agtataggag ttgactcagg gtcagtggca acaaagggtg tgctatttga tggtgaaaaa
     7381 attattaaga aaataattat accaactgga tggagcccta aaagtacatc taaacaggtc
     7441 tatgagttgc taagtagtga aattgacaaa aaagatataa aaaaggtggt aggtacaggt
     7501 tatggaaggg gagtaatgga ttttgctgat aagaaagtaa cagaaataac ttgtcatact
     7561 agaggtattt actttttaaa caagaacata aggactattt tagatgtagg tggtcaagat
     7621 agtaaagtta taaatttaga tagagatgga aatgtattta actttataat gaatgataag
     7681 tgtgctgcag gaactggtcg atttttagag attacttcta accttttagg aagcgatatt
     7741 gaaagtatag atacattagc taaaggatat gaaccagtta atatatctag tatgtgtaca
     7801 gtttttgctg agtctgaaat tgtaagtctt ctagctcaaa acatttctac tggagaggta
     7861 gcagcaggga tacttaaatc tattgcaaat aaatccacct caatgttagc aagaggtgag
     7921 gtaatagatg aagttgcatt tactggaggg cttgcaaaaa gtaaagaact agtaaaaatg
     7981 atagaagaaa tactgggtaa aaaaattttt attgcagaag atactcaaat aataggagct
     8041 ttaggagctg ctgtaatagg atttagataa aaacatataa aaagaaaatg agaagctgaa
     8101 tttattataa ataaattcag ttttttattt tattttattt atttataaag gaaaactttg
     8161 gtataattga tttaaatgat tgtatatttg taaatacttg atatttagtc atatataaca
     8221 ttataatttt aaaaattagc tcaaaataag ggggagaaag cgtgaaaaaa tttacttcaa
     8281 aaaaagtaac aaggtttgtt gcgctgtttt taatatctat tttagtaatg tcaacaattc
     8341 ctgtatcagc tgacaatagc aatacattga atcagataaa aaaagcagaa tacagtgaga
     8401 cgtataaaca atatctaaat gatgctaaaa atggatgtac agaaaaatat aatgggatta
     8461 taccaaatcc atatcagttg gaaggtactc aaatccaaag taaaggaaga acagctccaa
     8521 catcttatga ccctagaaaa ttaggtctta tgacatcaat aaaaaatcaa gaggatttag
     8581 gaatctgttg ggattttgct gcaatggcaa ccttagaaag tttcttaaag ttaaataatt
     8641 atggagacta tgatttatca gaagaacact taagatggtg ggcttcagat ggtgaatatg
     8701 gatggtctgt aaatgatatg aatggtgctt taaattatga agctatggga tatttaacat
     8761 cttggtcagg tcctaaatta gaaaaagata tcccatacaa tggtagagta tctaaagcac
     8821 aaggagcaaa aaaaccaact aatatgaata cagcacctac tgtttttaat gtgacagatg
     8881 ctgtgtgtgt aagcaatgac ataaattcta caaaaaatgc tatattacag tatggagctg
     8941 taacatctgg ttattatgaa gatattaaat atcaaagtga tgaccaaaat tcatattatt
     9001 gtccaacaaa aagttttaat acaaatcatg ctatttcaat tgtaggttgg gatgataact
     9061 attctaaaga taattttaat gcaaatatta gaccatcaaa aaatggtgct tggttaataa
     9121 aaaatagctg gggagattat aattctgagg gtggatattt ctggatatct tatgaagaca
     9181 agacacttat gtctgatata gataattttt caatcaaagg tggtaaaaaa ccaaatgatg
     9241 atgagaagat gtatcaacat gactatgcaa gtattgtacc tttaatttca aaaaagataa
     9301 cagctgcaaa tgtatttgat ttcaatagag gcgatgaaac tctaaaatct gtaatgttct
     9361 taactgaaag tataggagct aagtatgaag tatattatgc tccagtagta aatggtatac
     9421 ctcaagaaaa caatatgaaa aaattaaaag aaggtactgc ccaatattca ggatacataa
     9481 cagttccaat tgattctttt gatataccag aaggaaaggg tgcaattgta gtcagtatag
     9541 aggggaaaaa tggagagtca actataggtt ctgagtcaaa tgtaccagga tatgatacat
     9601 ttaaagcaaa agctaattta ggtgaaagtt atatcataga taatactgga aaattctttg
     9661 atataaatag agatagtaac ttctatccgt gtaattttac tataaaagct gttacagaaa
     9721 agtcttctgg tgagagtatc ccaaatgagt ctttaattgg tagtgataga tatgaaactg
     9781 ctatcaaagt tagtcaaaat ggtttcaatt cttcagaaaa tgtagtttta gtaaatggca
     9841 gttcaatagt agatgcattg gcagcaactc cttttacatc tgctataaat tcaccaatat
     9901 tattgacaca aaaagaagct ttaaattcaa aaactaaagc tgagatacag agattaggtg
     9961 ctaagaaagt atacctaata ggtggagaaa attctataag taaagaaatt gaacaacaat
    10021 taaaaagctt aaatatatcg atagagagaa tatcaggaag tgatagatac aagacaagtt
    10081 tattattagc tcaaaagtta aatggtataa aaaatgtatc tcaaatagca gttgtaaatg
    10141 gagtgaaagg attagctgat gcaattagtg taggcgctgc agcagcagaa aataacatac
    10201 caatcatact tgcaaatgaa aagagcgaat tgcaaggagc tgatgaattt ctaaattcat
    10261 taaatataga gaaatcttat attattggag gaacagcatc cctatcaaac aatctagaaa
    10321 gtaagttaaa aaatccaact agattatctg gtagtagtag agacgaaact aattcaaaga
    10381 taatagataa tttttataaa aaagatactt taaaaaatgc atttgtagtt aaaaatggaa
    10441 tcaagaatca aaatgattta atagatggat tggcagttgg tcctttagga gcaaaaactg
    10501 ggtcaccagt aattttagtt ggtgataaat tagctgatag ccaaaaagaa gttcttaaaa
    10561 ataagacttt agaaaaagta actcaagttg gtggaggagc aaataaaaat gcatttaaag
    10621 agttagtaaa attaaaaagt gctaaataga gtacttaagt attaacatat attagtgtgt
    10681 attagtaact tatcttaata aaagctgaaa ataattactt caaattattg ataaataaga
    10741 taatcaatga ttgtaaagtt ttgttttcag ctttaaattt atgtagtata agtttaaaaa
    10801 cataaaaaag ggttacttta tatagaaata tagaaaatct tacattttat atataaataa
    10861 cccatattta attaactttt tatagtgttc taatagatat tattgaactt aatggtaaat
    10921 ataatgtgta agtttcattt aaagttggat tagattcatc tatatatctt gagcaaagtg
    10981 taattgataa gctatctgct tctataattt caccatatag aggacaactg ctaaagtaag
    11041 aatgtacttc aacatattta tttaagtttt cttgaacata atcgtaaaaa cggttttttg
    11101 tagtagtagc cataaaatca actcctttat tttttattaa agttgaaact tttaaaaaga
    11161 taaatatgat tacttctatt agaaactaag ttaaaaaata agcaaataga tatgaaaatt
    11221 agaataatat attatttata tcttaattat aacactagat aataaaaaat gctatataaa
    11281 attcagaatt ttcgaaagtt tttaagctta tttttaaata tactaataat actattgaca
    11341 ttagatagaa aaagtattat attgactata taaattattt tagtcaaact ggagggaaaa
    11401 gatgcctaaa tcatattctg ataaagaaag agagtatata ataaaaagac ttaaagaaga
    11461 agcaagattg tgtatggagc aatatggtat tagaaaaact actgttgatg agcttgtaaa
    11521 acgggtaaaa ataccaaaag gaacttttta tctatttttt caatcaaaag aattactctt
    11581 ttttgaggta ttaagagata tacatgactc tatacaaaaa gaaatcttat atgagataaa
    11641 tagagtagat gaaagtatta catgtgaaca attgacagat atatttatga aattctatag
    11701 gatggttgat agtacttcaa ttttaaatct catgataaat ggagaatttg agatattggt
    11761 gagaaagtta ccagattcta taattgaaga atactttaga catgatgact ttgaaattgg
    11821 agagattata tcttgcatac caaatgcaaa aaataaagac atagaaagct ttagtggagc
    11881 atttagagca gtttttctta ccatgctata taagcgcgag gttggaagta attgttttga
    11941 agatgcttta agattaatga taaacggatt agttatacaa cttatggaat agaatttaaa
    12001 ttttgtttgc taataacttt tattaaaaat tattaacaaa caaaaaaatt atattttatt
    12061 gaccatatag tcaaaatagt caaaaatttg aaagggtgat attgaatgat taaagttgat
    12121 gatttatcat ttagttatac agatagagat tttttacaaa atattaactt tgaagtagga
    12181 aaaggggaaa tactaggatt tttaggacca tcaggtgcag gaaaatctac attacaaaag
    12241 attctaattg gaatgataac taattatgga ggaagtgtca ttgtaaatgg agtagagagt
    12301 aaaagacact caaataaatt ttatgaaaat ataggagttg attttgaatt tcctagttta
    12361 tatgaaaagt tgactgcaat agaaaattta aaatattttg ggtctctata ttctaaaaaa
    12421 ctactatcta ttgatgagtt attaaaatca gtaggactag aaaatgaatc aaataaaagg
    12481 gtatctgagt attcaaaagg tatgaaatct cgattaaatt ttataaaagc tttattacat
    12541 aatccagata ttttattttt agatgaacca acaagtggtc ttgacccatc aaatagcaag
    12601 gttatgaaag atataatttt atcagaaaaa tcgaaaggta agactattat tttaacaact
    12661 cataacatgt tagatgcaac agagctttgt gacagagtag cgttcattgt aaatggtaaa
    12721 atatctgctt tagatacacc acataatctt attatgtcaa aaggagctat taaagttaga
    12781 tatacttact ttgataatgg agaaaaaact tctgaatgtt ttctgaacaa tactgctaat
    12841 gataaaaatt tgaatatgct tattgagaaa aataaacttt tatcaataca tagtagtgaa
    12901 ccaacattaa atgatatttt tattgagata acagggagga atttacaatg aggctaaaaa
    12961 atcttatttt aggagatata aagtttcaat ttaagtatgg tttttatttt ttatatcttt
    13021 ttttaagtat tatatatatt tgtattatca atgtatttcc aacttttatg agagaaaaaa
    13081 tagctataat tatgatttat tctgacccag cagcaatggg attgtttttt atgggagcta
    13141 ttgtacttct tgagaaaagt caaagggttt taaattcctt agctgtatct ccagtaaaag
    13201 tatctgagta tattttatca aaggtaattt cattaggagt aatatctagt attgtagcta
    13261 tgtttattgc tattacatta aatttagata atattataat atcaactata ggtacctttt
    13321 tttcttctat tatattttct ttgcttggat tgataattgc aagtaaagca tctagtttga
    13381 atcagtttat tgttttaagt attcctattg agattatatg ctttattcca ccaatactga
    13441 atgtacttct tgacacaaaa tcatatgcaa atttgtaccc ttttaatata tgtatttctc
    13501 ttattagtgg agataaaaat tttataatga ttaatatttt aattttaata tcaattataa
    13561 taataattta ttttataact tattatttta tttgtagcag ttggaaaaag gtaggtggag
    13621 taaaactatg ataaagatta taaatgcgtt taaacaagag tttgaacaaa ttaagagaga
    13681 tgctatgctt tttatagtat gtgtttctcc aatattatgt ggagtattta taaaatttgg
    13741 aataccatta atacagaata tatcacttaa caagttttat tatcaattaa atttagagcc
    13801 gtatttcttg atgtttgatt tacttttagc ttttattaca ccttttatgt tcttttttgc
    13861 ttctacaatg gtcatattgg gagaaattga tgacagcatt tcaagatatt taatagtaac
    13921 tccgcttggt aaaactggtt atcttatttc acgatttgga ataccaggta tcttagcatt
    13981 tattattact atgattctac ttatcttttt ttctctaact aaaatttctc tcttgttaaa
    14041 cttatcaatt tctttattag ttttattaca aggagttatt atatcaatat tagtgatttc
    14101 tttatcatca aataaactag aaggaatggt aattacgaaa ttctcaggtg tttttatgat
    14161 gggtatttta gcaccatttt ttattttaaa taaagcacaa tatattctgt tcttcttacc
    14221 aactttttgg atatcgaagg catttaagga agataattat gtctatatgt ttatttcaat
    14281 tgttatatca ttaatatgga ttttattatt atttaaaaag tttagtaaaa aaatagttaa
    14341 ataagaaaat ttaatatata aatgtaaggt tgacttgaca gtaatgttga aaaatgttac
    14401 atttaagatg taaagttaac cttacatgaa tggagcaaat atgaaaaata aaataaagat
    14461 attaagagaa aaattaggat tgacacaaga acaactaggg agattagttg gaacatctag
    14521 acaggctata aatgctatag aaactggtaa aaatgaacct tcaatatggt tggcatatga
    14581 tatttcaaga atttttaatg aaccaattga aagtgtattt ttatttgaag aaagtgaaag
    14641 aaaatcaaga gcacagataa gtaggggaga atattatggc tataagaaag ataagaacat
    14701 ttgatgatga aattttaaga aaaaagagta agtatgtaga aaacgtagat aataagataa
    14761 gagaaatatt aaatgacatg gcagagacca tgtacaatac tccaaatgga ggaggattag
    14821 ctgcatgcca agtaggagta ttaaagcgat tagttgttat agatttagga gaaggactta
    14881 ttaagcttgt taatcctgaa attattgaac aagaagggga acaaattgtt gttgaaggat
    14941 gtttaagttt tcctgaagta tggggtaaat taaaaagacc aaaaaaagtt acagtgcaag
    15001 ctctaaacga atacggtgag aaaatagaaa ttaaaggttc aggttttatg gcaaaatgtc
    15061 tttgtcatga gatagaccat ttaaatggta tagtttttac agataaaatt attgaacatg
    15121 taaaattgta agatagattg gttattgaat ttataaaata ttttatagta aggagttaaa
    15181 ttatgagatt atggcataag gatttaattg atgtattacc taaaaatcaa ttagtaagtc
    15241 aatggagaga gctacttgct ataaaaggtt ctatagataa aaaaggtact ccaaaccatc
    15301 tattagtgaa taaagtattg aattatagta tagatgaatt taaattttat acaaaaattg
    15361 tacatgatga aatgttaaaa agaaattata aacctaatga acttaaatat acaagtatat
    15421 taaaatggaa aaatagaaat tttgcaaatg atatatctaa tgaacatagt ttaaatttag
    15481 aaaatttata tgatgattgg cataataaaa tgtatttaaa gcaatgctta tataatttag
    15541 aagaaaaagc aacatgtgga ggaataccaa taaatgaatg gaatatatta ctttgtaaat
    15601 atagtaaaga ttatgaattg tggtctggaa atatcatgtt ttaattacta tagattaaca
    15661 ttatagtatt attgtatata aagaggtgat tatttgtata ataaaataaa atatattcca
    15721 ataaattgtg aaatagcttg taataaatta aaaaggagta tgccatataa atgggattta
    15781 aatatttatc gtggatgtga gcatggatgt aaatattgtt atgctattta ctctcataag
    15841 tacatcaact ccaataatta ttttgaagag atttatgtaa aaacaaatat agtagaaaaa
    15901 cttgagagac aacttaaaag cagtaaatgg aagaaagaag taatcaatat aggaggagtt
    15961 acagatagtt atcaaccaat agaagctgac tataaaatta tgccagaaat attaaacctg
    16021 ctaataaaat acaagacacc agctattatt tctacaaagt cagatttaat tttaagagat
    16081 tacgatttaa ttgataagct atctaggatt acatatatca atgtagcatc tacaattacc
    16141 acagtaaatg aagatacaca aaggcttatt gagccaaatg gtgtggattc aatgagacga
    16201 tttaaaatgt taaaagaatt tagaaaaact aacgcatcag ttggtctaca tattatgcca
    16261 attataccat atattacaga tgattttgat aatataaatt cgctatttag acatgcaaaa
    16321 gaatgtaatg tacattatgt tttacctggg actttatact taaggggtat aactagaggt
    16381 gttttttttg actttgttaa aaaagagttt cctgaattat ttgataaatt atcaatacta
    16441 tatagtgcag gttcacttaa tagggagtat aaaaatcaat tgtataaaat ggttaatgaa
    16501 cttagatata agtattctct atcaagcagt tactctaaaa taatgaagga aaagttaaaa
    16561 aactcaggag atgtacagat atcttttttt gattagtttt atataaaaca aagtataaat
    16621 ttctaattat ctttttatat tttcttaaat gctcgtaaag ccttattcta tgtgctttcg
    16681 agtattttta ctgtaggaag atacttcacg tttctttgca tatttcctca tgtcttagct
    16741 gtcagaagtg gtaaataagt agtaaattca tttgtactac taagcaacaa gacgctcctg
    16801 ttgcttctct ttattcaagc gtttcatttc tgccattgca gaatcgaatg ttgcatgtgc
    16861 gtaatagttc agcgtcatgg ctatattagc atgtcccata atgtactgta atgcctttgg
    16921 attcattcct gcatttgcat agttggtaca gaatgtatgt cgcaaactat gtggagtgat
    16981 gtgtggcaat ttatcctcgt tatacttatt gtatttctta acaagacctt tcatcatgcc
    17041 gttgtaatca cttgccactt ttggatagtt ctttctatta agaaagagga aatcactata
    17101 tccatcaatc tcaacacgct tatcattctt tcgattcgct aacactcgct taaatgcttg
    17161 ataggcttct tcaaccatag gaacttgacg ttcgccactt ttggtctttg gtgtttcaat
    17221 gtagtaccca atttcagtat ctctcaatag ctgatggtct atattgacaa gacgattctc
    17281 aaaatctaaa tctggaagtg tcaaaccacc aaactctgaa atacgaagac ctgtttttaa
    17341 gagtatcaga atttcatcat aatttttgct gtaggtttta tcagcttttg caaaggctaa
    17401 cagtttttct tcctgttctt ctgttagtac ggtcttaggg acagtatcat catcaagaac
    17461 tgctttcagt tgaaagtcaa atggattctt ccgaacacaa tcatcttgta tagcaatata
    17521 gaatgaagcc tttaaagaac gtttgtagtt attgatggtt tgataagcat aaccattttc
    17581 actcattcta atagcccatt ctttagcgtc tgatggctta atactgtcaa tacttcttac
    17641 acctaacttg tctttcttca aaatatccat aagatatttg cgtccagttt cagtgttttt
    17701 tctaaccttt ggtctttgag cgttctgttt tgcgtaaagc tggcagagtg tcattttctt
    17761 tcctacaaca tcaataccat catgaatgtc tttctgtaac tctgcgattt tctctctaag
    17821 tgagatacaa tcacgctttc ctgctggtac tcggtctgta gccacaagtt tccacgagta
    17881 aacaaattgc ggttctccaa atgaatctat atatttgtat aagtatcttc cgtcttttcg
    17941 ttggctctct ccagtcttta agattcgacc tttattgtca cgtctttttt ctgacatggc
    18001 atttgctcct ttcctttatg gaaagagcct tgatacgact taatactatt ttatcatata
    18061 caagaccctt tggcgacgct agattgcgtc caatgtatct ataatttttt caaattgttt
    18121 tcgtttaatc tgaatacgat tgccattcat aatcagccaa tttgcatttt tattttcctc
    18181 tgccaagcgt cgtagcttgt tttcgccaat acgaaaatat tttgacgctt cttcaatggt
    18241 tagggtataa cgttcccaaa taggaatgtc agtctgcttc ataaaatcct cctttccaaa
    18301 tcacttattt ggatttcata aaagttgttt taccagcaat cgaacagctt tagcaaagct
    18361 cacgggagtt ccacccctgc atggttctca tgtagccata ctcattgcct gcgacggttt
    18421 tatcacgctc ggactattga ctgtatggga gtatcattat cacgataaga atgtcgttgc
    18481 aggcaatcct gctaaagatt gcttctcgga tcactaacat gaatcgctcg ctatctttat
    18541 aagataggtc atggcggtta gttccgttgg ctcttttctt atcgaaacgt attcgattac
    18601 ttttattcag ttttcaaaga acaatggctc gttagcctat caaaacacat tgaaagctca
    18661 atatgctttg gtggaataac aaacctccct gttcgggaag cgtggaatgg tttagcacgc
    18721 ttccacgaaa ggagagagga tattacttaa tttcaaatga caaaatcttt gtaatcagtc
    18781 tggtttccat tcttccacgt aagacttcat caacgaccat actttgattg ccatattcat
    18841 ctttcataag tcgtagggaa cgcttcgtta tgtaccctct gtaatgatgt agaatctggt
    18901 taatcgcttc ggtatcgcca tctgttgcct ttacaatgag aggaaaggga atcataggat
    18961 attgtgtttt cattcttcaa attcctccat aaacttttta attaaggcta gtccactggt
    19021 tctatgccga tagacagtag aacggttcaa tttcaacagg tctgcaattt ctgaatcgct
    19081 catgtccata aagtaaaaca gcagtagaat ttcacgtttc ttgtctggca actcacgtaa
    19141 tgcttcactc aacaaatcat tttcaacgcc tactgataac ccattgagtg taaaaatctg
    19201 aaagtcagtt gaatagttat ctgttgtcgc aaactggcta acaagataat cgccaacatc
    19261 cgaaaaggac acctcacgct ttgcaatcct tgaaagataa agcatataat tctttcgctc
    19321 gtcttccata gcacgtttac agatatagtc aaactgattt tctattgtgg tctgaaaaga
    19381 agatggtttc atgtttctca ccccctttct gtctaggaaa ggaagtgagc cttgctcgtt
    19441 tatctccttt cactcttagt cccaatgtga aagggggatt tgttgcatta ctgataaata
    19501 aactttgtaa aaaagttctg aatagccaaa aaagcatata aacagattta tttctctgtt
    19561 tacatgcttc tgttattcta tctatatgat ttataaaacc acattggtgg acgtacttat
    19621 ctattgcaga tagacgactt tttttgacaa gaacccaatg taaggaaatt tattgtatat
    19681 gatgtacttc atggcgacgt tgacctccaa caaaccgcca tttggaagta atatacaata
    19741 ttttaacagc gtaaatagca ctaccatata acggtttttt ttattggcgt ttagtagtgc
    19801 tttttattaa atataaacct ataaaccata taacacgttt ttctatacct gtttttaatt
    19861 cagtaggaac aataaaatgt atagaggtgg tctactatgc gtaaaaaaga agataaatat
    19921 gattttagag cctttggttt agccattaaa gaagctcgat tgaaacgagg tttaactcgt
    19981 gaacaagtgg gagcattgat tgaaattgac ccacggtact taactaatat tgaaaataaa
    20041 gggcaacacc ccagcataca agttctttat gaccttgtat cgttacttca tgtttccgtt
    20101 gatgaatttt tcttacctgc taataacttg gtaaaaagca cccgacgatt acagatagag
    20161 aaatacatgg atagctttac agacaaagaa ctatccttaa tggaatcttt agccagcggt
    20221 atcaacgaag caagaaacat cgaagactaa ttaaaagaat ccatacataa cggaaagagc
    20281 cgataaaatg agattgtatt aatctcattt tatcggctct gcgtctttgc gtctggctct
    20341 gtaatcacag ttactttgaa ctgctttatt tcaattaaat tttcttgtct gcatttcgga
    20401 caatagaggg ggaatttttt taattcagta tcttccctta tctttaatcg tgttttattt
    20461 ccacatacag gacacaatat ccacttgtag tttataataa ctatctcctc ctttacactt
    20521 taattcaaat ctttattaaa aaatatttca tcttatttaa caagaaacca tatttatata
    20581 acaacataaa atacactaag ttattttatt gaacatatat cgtactttat ctatccgact
    20641 atttggacga cggggctggc aaacaggttc accggtagta acatggtacc cttttaactc
    20701 tgttaaacaa acactacgtc catttgtaaa gaaagttaaa tcactacgat attcttgaat
    20761 acaccgagca gggatttctc cactaagaat gacctcatta tttttcaatt gagtgtctac
    20821 gatgttcgca caatatttag gagcatcgtt gtatgctcgt gaaagatatt cctgtggcgc
    20881 ataaatttta aaactaagat atggctctaa caattctgtt ccagcttttt ttaagacttg
    20941 ttccaataca ataggagcaa gcatccgaaa atctgctggg gtactaacag ggctatagta
    21001 taagccatac ttaaaacaga ttttacaatc cgtcacattc caaccatata atccttgttc
    21061 gcaaccatag cgtatccctt ccataactgc attttgaaat gattgattta agtatccaag
    21121 agaaaccgag ctctcatact gcattccact tcccaacgga agcggtgata cagataaacc
    21181 aatggaagcc cagaaaggat ttggcggcac ttcgatgtga atggtatatt ctgcattttt
    21241 taacggtctc tccatataaa tgactgtagg ctcttttagt tctatctcca catgatactt
    21301 ttcttgcaac agtgcactaa tcacttccat ttgtactttc cctaagaaag aaagtataat
    21361 ttcatgtgtc gtagaatcca cgtaatatcg tagaagcgga tcactatctg agatttccaa
    21421 aagggcatca agcaacattt ctctctgttc aggtttactc ggttcaacag ttgtttgtag
    21481 tagagggtgc ggattttcaa tcttttttct ctgtggcaat agttttgtat ctccaagaac
    21541 actatttaac ttcaaaaact cattttgcaa aataacaatt tctccagaat aagctctatc
    21601 aatcttacat aattcaccat ttattgaagt atacatttct gtaactttta ttttttcttt
    21661 ttctgatact ctaaccgaat ctcgtaaatg tagtactcca ctataaaggc gtatatatgc
    21721 aagacgttgt cttttttttg tatattcaat tttgaaaaca tttccgcaaa gttcagacgg
    21781 acctcgatgt gttgatgaat aaaatttatt cgtaatcact tctataaggt tatcaatccc
    21841 tatattgttt tttgcacttc cgtgataaac agggaacagg gaacaattat gaaatcttat
    21901 gctttcctct tgttcgagtt ccaatgcttc taatgattta ccggacatat atttctctaa
    21961 aaggtcatcg tttccctcta ttaccgtatc ccattgttca gattcggtaa agttcgtcac
    22021 acacatatta ggatacagtt ctaccttctg tttgattaca atttcggcag aaagtttctc
    22081 tttaatatcc tgataaaccg ttgataaatc aattccattt tggtcaatct tattgataaa
    22141 aaagattgtg ggaatcccca ttttcctaag tgcatgaaat aatatacgag tttgtgcttg
    22201 tacgccatct tttgcagaaa tcagtagaat tgccccatct aaaactgata atgaacgata
    22261 tacttctgct aagaaatcca tatgtcctgg cgtgtctatg atgttcacct tcgtattttc
    22321 ccactgaaaa gaggttattc ctgtctgaat tgtaattcct ctctgacgtt ctaaaagcgt
    22381 attatccgtc ctcgttgtac ctttgtccac gcttcctaat tctgtaatcg ctccactgtt
    22441 atataataag ctttctgtta aggtagtttt tcctgcatca acatgagcta aaactccaat
    22501 attaataatt ttcatgtgat tttcctccat tcaaaaaccc aaaagggcat aaaaatccca
    22561 gtgataaata cttttatcac tgggattttt atgcataacc ataggtatac aaagcataca
    22621 gatattctct ggatacttta gaatcacatg ataaaggtat tcttaaactg ggtacaaaaa
    22681 actaagccct ggtatattgt aattgactta tctatctgtc aattatatac accttttcct
    22741 ttcattaaat tttgtttaga attgaattgt gaatgtgtac tttttctttc agcacctgta
    22801 gcttgactac tt
//
LOCUS       DAEOSP010000044        22559 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.044, whole genome shotgun sequence.
ACCESSION   DAEOSP010000044 DAEOSP010000000
VERSION     DAEOSP010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22559
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.044"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            33..686
                     /locus_tag="KRH54_001395"
     CDS             33..686
                     /locus_tag="KRH54_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG8579062.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            827..2098
                     /gene="brnQ"
                     /locus_tag="KRH54_001396"
     CDS             827..2098
                     /gene="brnQ"
                     /locus_tag="KRH54_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG8579063.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            2300..3487
                     /locus_tag="KRH54_001397"
     CDS             2300..3487
                     /locus_tag="KRH54_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBG8579064.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(3717..5741)
                     /locus_tag="KRH54_001398"
     CDS             complement(3717..5741)
                     /locus_tag="KRH54_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8579065.1"
                     /translation="MDKKKLILITHDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLGLDEKLIKSPSLMEYMPNSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCIEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            6131..6799
                     /locus_tag="KRH54_001399"
     CDS             6131..6799
                     /locus_tag="KRH54_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579066.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            6801..7286
                     /locus_tag="KRH54_001400"
     CDS             6801..7286
                     /locus_tag="KRH54_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG8579067.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            7347..8468
                     /locus_tag="KRH54_001401"
     CDS             7347..8468
                     /locus_tag="KRH54_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBG8579068.1"
                     /translation="METNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGIAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            8749..9939
                     /locus_tag="KRH54_001402"
     CDS             8749..9939
                     /locus_tag="KRH54_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG8579069.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    10073..10328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10387..11388
                     /locus_tag="KRH54_001403"
     CDS             10387..11388
                     /locus_tag="KRH54_001403"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBG8579070.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            11492..11881
                     /locus_tag="KRH54_001404"
     CDS             11492..11881
                     /locus_tag="KRH54_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBG8579071.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            12042..13250
                     /locus_tag="KRH54_001405"
     CDS             12042..13250
                     /locus_tag="KRH54_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBG8579072.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(13336..14412)
                     /locus_tag="KRH54_001406"
     CDS             complement(13336..14412)
                     /locus_tag="KRH54_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG8579073.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            14641..15168
                     /gene="hpt"
                     /locus_tag="KRH54_001407"
     CDS             14641..15168
                     /gene="hpt"
                     /locus_tag="KRH54_001407"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG8579074.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            15257..16189
                     /locus_tag="KRH54_001408"
     CDS             15257..16189
                     /locus_tag="KRH54_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBG8579075.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            16251..17477
                     /locus_tag="KRH54_001409"
     CDS             16251..17477
                     /locus_tag="KRH54_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG8579076.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            17561..17782
                     /locus_tag="KRH54_001410"
     CDS             17561..17782
                     /locus_tag="KRH54_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG8579077.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            17971..18141
                     /locus_tag="KRH54_001411"
     CDS             17971..18141
                     /locus_tag="KRH54_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579078.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(18234..18695)
                     /locus_tag="KRH54_001412"
     CDS             complement(18234..18695)
                     /locus_tag="KRH54_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579079.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            18878..19345
                     /locus_tag="KRH54_001413"
     CDS             18878..19345
                     /locus_tag="KRH54_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579080.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            19388..21151
                     /locus_tag="KRH54_001414"
     CDS             19388..21151
                     /locus_tag="KRH54_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBG8579081.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNDTIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            21159..21767
                     /locus_tag="KRH54_001415"
     CDS             21159..21767
                     /locus_tag="KRH54_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG8579082.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            21839..22408
                     /locus_tag="KRH54_001416"
     CDS             21839..22408
                     /locus_tag="KRH54_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBG8579083.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT         8588 a         2389 c         3862 g         7720 t
ORIGIN      
        1 caaaaataat ataatttata gtggagggga aaatgactaa aattattaaa gtgcaaaaac
       61 ctaaatttac aggagaatta gaaattattg aaaatcttga agatatactt gaagatatat
      121 ttaatgatga aaaaatattt aataaaatta tagaaaatga agttataagt gatttagatt
      181 ctgtaagagt tagctttgaa tcttgtatat ttagaaatgt gtcttttgaa aattgtaatt
      241 taaaaaagat tgatttactt gatgtggtat ttgaaaactg caatttatca aatacatttt
      301 ttgatggagg aagtatatac agagttgaat ttaaaaatag taaattgata ggagttagat
      361 ttgatgattg tatacttaaa aacgttttgt tcaaggatgt tttaggtaag tattctaact
      421 tttcattctc taaatttaag atagtttcta tcttagattc aaattttgaa aattctgtat
      481 ttcaagaagt aaaaggtgac aatctagtat ttgagtatac aaatttaaaa aaagcaaact
      541 ttaatggaac taaactttct ggcatagatt ttactactaa taatattgag ggaattgaga
      601 ttggtataga tgatattaga ggagcaattt ttgatgtgtc acaagcaatt gatttaacga
      661 aattgatggg aattatcata aaatgaaaaa aatattcata aaataaattg ggaaaacgtt
      721 aacaatgtgt gaatttttac cagattgaca tttttctgaa aataaagtaa gattatctta
      781 atattcaaaa tattttcaaa gggggtaatt tagtgtgagt aagcagatga gagatatttt
      841 tatagttggt tttgctttat tttctatgtt ctttggtgca ggaaatctta tgtttcctcc
      901 atttttaggg atggaatccg gcaaagactg gttaattcct cttattggat ttgttgttgc
      961 agatgctggt atagcaatgc ttgttattat agcaacagca agatgtaagg gaagcatgga
     1021 tgatgtactt aggagagctg gtaaagggtt agcaagagta atgagtgtag ctgctctagc
     1081 atgtttagca cttcttgtaa taccaagaac ttgcgcaaca acatatgaaa tgggtattat
     1141 gcctatattt ggagaccatg gtccagtagc taaagctgta ttttcaataa tcttctttgg
     1201 gctaacttta gcatttacta ttaggtcttc taaggttata gacataatag gtaagtattt
     1261 aactccagcg ttattattag tattatttgt tttaatagta aagggtataa tttctccagc
     1321 aggacctatg agtcctgagc atatgataga taaaaatcta tttggtgaag gtatatcaca
     1381 aggttatcaa acaatggacg ctctaggtgc agcatctatg gcaactataa tgttgatgtc
     1441 tataatagct aaaggatata catcagataa agaccaaata ggaatgacta ttaaagctgg
     1501 ttttgtagcc tgtgtatttc tagcagtagt atatggtggt cttacttatt taggtgcaac
     1561 agtttcaact ttatatgata ctagtatacc tcaagcatct ttattagttg aaataactag
     1621 tatattgtta tatggtcaag taggaaaggt aattttaggt gtaattgtgg cacttgcctg
     1681 tctgactact tctacaggtt taacagcatc aatatcaact tattttgaag gaataagtaa
     1741 ggcgagttat aaacaactgg ttataggtat atgtgtagct agtatgctta tatcaaattt
     1801 aggtgttgat tctataatag ccatatcagt tccaatccta caaactatat atcctgtttt
     1861 attagcaata gttgttatgg agttggctgg taaacatata aagaatgata atgcttttaa
     1921 aggtgcagca tatgttacat tagtgattag tttattatca gctataaatg gaatgacagg
     1981 agctgttcca tttatacaaa gcttgccact tgcaggtttg ggctttaact gggtaatacc
     2041 agctatattg ggtggaatag ttggtaactt tataaaaagc aacaaacaag tagcataata
     2101 atttgtaatt aatttaattt tataatatat taatttttag tactatgggt aggtttaatc
     2161 taaacctaat ctacccatag tactttttag attggattat aatagcatgt atataatttt
     2221 ttataatata atttttcttt gaagtatgat aaaattagta atagtgataa gaatgtacaa
     2281 atttaggagg ttttaattaa tgcttagaaa aataacagca tttatgctat ccatatgttt
     2341 aattattgga cttacaggtt gtagtttact tgtaggcaag aaagataaag agaaaattat
     2401 agatgatttt aaagttgcag tggtaactca accattatct gaaaataaag tacaatacaa
     2461 tatggtggaa gagatggcaa aagaatatga ggaagagaat aaaattgata aagacaaaga
     2521 tggtcaaact aaagttaaac aaactataaa gcatgttgta ttgccagaga attttacttc
     2581 taatatagac agtgctatta ataagatagt aaagttggct gacgataaag aagttcaggc
     2641 tattgtagtt tcaactgacc aagcagggct tttacctgct ttacaaaagg taaaggaaaa
     2701 gcgtccagaa ataataacta tttctgcccc tatgggagat gataaaaacc aattaagcca
     2761 atttgttgat gtgaatctag gggttagtgc agaggaaaga ggaaaagtat tagctgaacg
     2821 ttcaaaagaa atgggagcaa aagcttttat tcattatgcc tctacagatg atttgaaaga
     2881 tgttaatata gccaaaagat tagaaatgat taaagagact tgcaaaaata taggattgcc
     2941 atttgtacaa gttaatactc caaatataaa tactgaagaa gacaaaaaca aagttaagca
     3001 gtttttaaat gaagatattg aaaaacaagt taaaaaatat ggaaaagata taaatgtatt
     3061 tggtgtaaat gagtatatgg atgaagttat attgactaag gctttagaat taaagtatat
     3121 agttgcagag caaagcaatc catcaccaat acaaacatat cctagtgtta tgggtctaaa
     3181 aatatctgaa aaagatgctc aaaattatga taaaataaac gacatgataa gtgaaaaggc
     3241 taaggctttt ggcatgtcaa atagacttgg tggatatcct atgccaatgg atgcttttct
     3301 accatcagta gcaatttatc ttgctactga aatggtgaaa caagatttaa cacaagaaga
     3361 tgtatgtgac ccagattatt tagaggcgtt tacagaatta agatttggaa tcggttcaga
     3421 atttactcct ttaacagagg tactttataa ttatcaatca gtaattttaa gccaattaat
     3481 ttattaatat taaggaagtg tctcaaaatg aactttttag tttatgaggc actctttttc
     3541 tattaaatca aatatatttt tatcttttta acaattaaaa agaagcttat ccctatattg
     3601 agatagcttc gtttttaata taaataagtt ttattatttt ataaaaaatt tatagaaaaa
     3661 taaaaaatta taattatata taaattttga taaagtaaag atgaattact ataggtttaa
     3721 attatattat tttctattat ttttatgcct ttctcagtta ttgatgagcc ttttcttcct
     3781 ttactagaaa ttaagtatcc attgcgtgtc aaatattcta gcatatttct tacttcgtat
     3841 tctgtgacat tatattccat aaatttcatt aactttgtcc tccccattgg tttcatgtgc
     3901 aatgatttta ggatagaaat gcatatgttt ttgtcatatt ggtttaaatc attgaatatg
     3961 gacatattac ttgacatatg attattattt aattttgaag atatgttttg aggtaaatca
     4021 tttattgtaa gataattgga acccatgtaa gccatatatt caatacaatt tctcagttct
     4081 ctaatattcc ccatccaagt gtatgaattt aaagcttcta aaagactctt atcgagtttt
     4141 atgtttttat agggaagttc ttttaaaaat gattctaaaa gaagaggtat atcttctaat
     4201 ctctttctaa gaggaggtaa atctaaagtg aacatactta tcctataaaa taaatccatc
     4261 ctaaatttat tttctataat taatttctct aaatctttat ttgtggcagc taatattctc
     4321 acatcaattg gaataatact tgtaccacca atttttctaa tttctttttc ttgtaagact
     4381 cttaaaagtt taacctgtaa atcatgtggc atatctccta tctcatctaa aaatatagtg
     4441 ccagtgtgag ctaattcgaa taatcctttt tttccacctt tttttgctcc agtaaaagaa
     4501 ccatcttcat atccaaatag ttcactttca agtaattcac ttggtaatga agcacaattt
     4561 attgctacaa aaggtttatt ttttctattt gaatgattgt gtattgactg agtaaaggct
     4621 tctttacctg ttcctgtttc acctgttatt aatataggat tatctgtaag agccattttt
     4681 tttgcctttt taatacaatc ttttataata gaactatttc caataatatt attaaattta
     4741 tatttagcta cataacctct tttgacagaa tcacttctta ttttttgttc taatatttct
     4801 atattgttta catctttcaa tgtaattaag caaccctcta tatttttata aagataaaat
     4861 ggttttttag aaagtatgaa ttttttatct atttcgtcta tgtatataat catattatcg
     4921 atatttaagt tctttgtaat tttatctaag aaactatttg gcatatattc cattaaggaa
     4981 ggtgatttta taagtttttc atctagtccc aacatattaa gtaaagactt atttacaaaa
     5041 gtaaccttgt taaaggtgtt gaaaaatatt agaccatcac ctatttcatc aatggtcatg
     5101 taaagtatag tttttaattt agatatatta tctaatgatg tatttaagaa atcatgactt
     5161 agtgtctctt tggacaattt gtatagtttt tctataaatt tctcattttt tagttttaat
     5221 actactaata atgacatata tgtatctaat gagatttttc tccaacctaa atcaacaata
     5281 ttttttatgt attttggtat aaatctaatc tgacctggtg ttatagctgt atctaaatca
     5341 tatatttcat cacaatctgg atatgtcggt acaaaatcta tatgatttat accaatttta
     5401 tatagataag tagccatctc aaatgctgaa gttttattgg ttgtcataag taacgatttt
     5461 gtacctggag gtagtttcat cattttttca tatccaagtt ttgaaagtgc tttcgttcca
     5521 tgaataattt caatatcttc cttaaaatgt tttattaaaa aattactagt taaaggtgat
     5581 agagaaagca ctacatctcc ctctaaattt tcttttattc catcaagtat attttgtgtt
     5641 tctatgatta tttcgtctcc aaaaaagttt gttaattctt ctttgtagtg gtcactattc
     5701 aatttatcat gtgttataag gattaacttc tttttatcca ttttatttct cctaacaaaa
     5761 tagtatttat ttttaaagta tttaaagtat aaggcaagtt atattataca acaaatagta
     5821 gtataaattt atatatttga ttaaataata ggttttttta ggattttatc ctataaaaac
     5881 ctattttaaa attatttttg caagataagg aaaatagctt tttatattgt aatgtatatt
     5941 ttaaataatt aataaaaaaa tattatattt ttaaacttga aaagtaagta attataagct
     6001 atttaatcac tggaataaat ttacaataaa aaaataaata caaaggcttt atcattaagg
     6061 tattggcata agttttgctt attaaacaag tatactaata aaattcattt aaacaggagg
     6121 gagaatttat atgagtaaat tagaatcaac aacagaaaaa gttgaggtaa aatggtatgg
     6181 gtatgtagca ttaatattgg gagcattatt tttttcagga atatttaaag atgcaccagg
     6241 accattaaaa gtattggatt ttaacaatgt attaggttct tttggaacat tgggaacaat
     6301 taatgatggt gtaggtacat tagctgcaaa ctttagaggt gatggaggta ctggccctcg
     6361 agatggatgg ctatatgcat tgacattaat accatcagtt atgtttgcac ttggagtagt
     6421 tagagttata gaccacttag atggaatgaa ggcagctcaa aagcttctta gtccactatt
     6481 aaagccatta ttagggttac ctggatttgc aggactaact ttgatagcta gtttacagtc
     6541 tacagatgca gctgcatcta tgactaaaga acttaaagat gatgggtata tagatgaaaa
     6601 acaaaaagct gtattttgtg catttcaatt ttcaggtgcg tctgcaatta cgaatttctt
     6661 tgcttctgga gcagcactat ttccatttat aggagatgta ccaatattta taccacttgc
     6721 tttaatatta attatgaagt ttgttggggc gaacttactt agattatatt taaataaatt
     6781 tgacaagggg gaagcttaac atgagtgagg tagcaaaaaa agaagtaaaa gttaaaaaag
     6841 gaaatgtgat agatgcgttt attggaggag ctagaaatgg attccaaata agtacaaatt
     6901 caatggctcc aaatgtcatc ttcggttttg ctataatttc tgtgttcaat ttaacaggtc
     6961 ttttggatgt aatagggaca atatttacac ctgtaatgag tatctttgga cttccaggtg
     7021 ttgcagcaac agctataatg acaatattta tatcaatggg aggagcatct ggagtaatcg
     7081 caggtttatt tacagcaggt caaataagtc aagcacatgt tgcaattcta ttgccaatgt
     7141 tattgctttc aggagcgcag atacaattta tggggagact tttaggaaca gctgagttaa
     7201 aaactaaata ttatccacat atgtttgtta tagcaacttt aaatggttgt attgcaatgc
     7261 ttataatgaa ttatttcgta gtataggtaa ttataataaa gatttaatat aaatggaaag
     7321 tttaaatttt aaggaggagc tagattatgg aaactaacaa agactatatt gagtatgtac
     7381 ataaagtgta tgattatcta cacactatac cagaaaaagg atttgaagaa tataagacat
     7441 cagagtttgt tttaaaagag cttgaaaaaa tggggtttga aaatattaat aaaggaattg
     7501 caggaactgg tattatagta actatagatt ctaaaaaaga aggaccagta tttgctataa
     7561 gagcagatat ggatgcattg gaatttatca tagatggaga aaagaaaatg atacatgctt
     7621 gtggtcatga tggtcatact tctatgcttt taacagctgc aaagttaata aaagagagaa
     7681 atttagtaaa aagaggtaag ttaaaaatac tttttcaacc aggtgaagaa aaattatatg
     7741 gagcattaag agtaattgat tctggtttgt tggatgatgt agaagaaatg gtagggatgc
     7801 atttaagacc tggacaagat tgtaggttgg gaaaggcgat acctgcaatg taccattgtg
     7861 ctagctgtat aatggaattt acgattaaag gtatcacttg tcatggttct aaaccacatt
     7921 taggagtaaa tgcagtagat gctgtctgtt tagcagtgaa tgcaataaat gcaataagag
     7981 taaatccagc tataccaaat tctataaaag taacaaatat atcagttaag ggagaaactt
     8041 ataataatat accagatgaa gcatttatgg ctttagatat aagggcagaa agtaatgata
     8101 taatagaaga attaaaagaa aaagcaatac atgctgtaaa aactgctgta agttctataa
     8161 atgcagaagc tgtactaatt tccaaagatg gagtaccagc agcagaattt gatgaagaac
     8221 ttgtaaactt ggctgaagaa actataaaag atgtattggg agctgatgca tctataggta
     8281 tatttaaaaa ttctggtgga gaagatttcc attattatac acaaaaacta aaatgtaaaa
     8341 ctacatatat tggattagga gctgatgcta caccaggatt tcataatcca aatgtaacat
     8401 ttgatacgaa agctttagaa tatggtgtag atatatggtg tagattggtt gaaaagagat
     8461 tgggataaca tccaaactaa taattctaca tttaaaatta taattataga agttatagaa
     8521 aaagattgct tatgttaatt agaaaataat taatatgagc aatcttattt tgatataatg
     8581 aaactgattt tggaatattg aaaaaatatg atggaaaagt aattatattt taaatctatt
     8641 ggacaaaaaa gtgatattat tataatatta agaattgtaa aataatacca aagtgcatct
     8701 agtaatagac ttaacaatta taaaaatttt attcttagga gggaaaaaat gagtaagtat
     8761 aattttgaca gagtaataga tagagtagga acagattgtg taaaatggga ttttagaact
     8821 aattgttcaa caaaagcaca aaaagatggt ttgccttttt ggattgctga tatggatttt
     8881 gaatgtgcag aaccaataat agaagcatta cgtaaaaaag ttgaacataa aatatttgga
     8941 tatagttcaa atgatagtga taaatacttt aatgcagttt gtgattggta taaaaggaga
     9001 tttaactggg aaataaatag aaaagatatt atttttagtc ctggtgttgt acctgctgtt
     9061 gctattttag taagaatatt aactaattct aatgaaggtg taatagtcca aaaaccagtg
     9121 tattacccat ttgaagctaa gataaagagc aataataggg atgttgtaaa caatcctctg
     9181 atatatgaga atggtactta taggatggat tatgatgatt tagaagaaaa agctaagtgt
     9241 agcaacaata aagtactgat actttgtagt cctcacaacc ctgttggaag agtttggaga
     9301 gaagatgaat taaaaaaggt tgtagaaata tgtaaaaaat atgacctatg gataatagct
     9361 gatgagatac attctgattt aattagaaaa ggatttaaac atactccttt acagtcatta
     9421 tgcccagagt acaaggataa aatagttaca tgtatagctc caagtaaaac atttaatctg
     9481 gcaggaatgc agttgtctaa tataatagtg aataatgatg aactaaaaaa gaaatatcaa
     9541 gaagaggtta cagctgttgg agttgcaact tcaccaaatc cctttgctat tgtagctaca
     9601 atagcagcat ataatgaaag tgaagactgg cttaatgaat taaatgatta cttagataat
     9661 aatatacaat ttattgatga atacttaaaa gaaaacctac caaaagtaaa acttgtttat
     9721 ccagaaggaa catatctagc atggttagat tttactgctt atggattaaa tgaagtagaa
     9781 cttgaagatt taatgtttaa aaaagctaat gtattatttg atgaaggtta tatatttgga
     9841 aaagaaggaa taggatttga aagaataaat gtagcttgtc cacagtcttt attaaaagaa
     9901 tgtatggaca gattaaagac tatatttgaa aatttataaa gtataggtaa aaatttataa
     9961 agtgtttaaa aataaaaaaa taataaactt gacaaatttt aactaatcac ttatcatact
    10021 attatgaata aatagaaaac ataagagagc taaattttat tgaattaaac tcttaaagac
    10081 tttgaaagga agagtagtta tagaatgtaa ttaaagcgag ttggtgatgg tgtgagacca
    10141 atatgaagtc tataatgaag aacatcctgg agttgctaac cgaaatttta aaagagtagg
    10201 cttagtcgga accaaaccgt tatcatattg gggtgtatcg agtaatatct gtacattgtg
    10261 agctgggttt gaatttattt caaactaatc agggtggtac cgcggaaaag caacctttcg
    10321 tcccttttat atagggatga agggtttttt taatggaaaa atatataaat atagggagtg
    10381 aatgatatga gcacaataat accaaaggat tataaaagta gtttgaacgt aattgataca
    10441 caaatagcta taaaaaaatt aaaagatttt tttgaaatga gactaagtaa tgaattaaat
    10501 cttataagag tttcttctcc gttatttgta ttgccagaaa caggtgcaaa tgataattta
    10561 aatggagaaa aggctgtaag ctttgatata ccatttatga ataaaaatgc agaaattgtt
    10621 caatcacttg caaaatggaa aagattagcc cttaaaaaat atgggtttga agttggtagt
    10681 ggtttatata cagatatgaa tgcaataaga aaaaatgaag aattagacaa tatacattct
    10741 ctttatgtag accaatggga ttgggaattg gttatagata aaagtagtag aaatgaaaag
    10801 actctaaaag atgttgtaaa aagattgtat ggagtattta aagatactga aatttttgta
    10861 tgtagtatgt atgaaggtat aaaagaaata ttaccagaag aaataacttt tataacatca
    10921 caagagttag aagatatgta tccagaatta tcaccaaagg aaagagaaga taaaattgta
    10981 aaagagaaga aagcagtatt tataactcaa ataggaaaaa cattaatatc aggagaaaaa
    11041 catgatggaa gaagccctga ttatgatgat tgggaattga atggggatat attattttac
    11101 aatcctgttt tagattcagc attagaatta tcttcaatgg gaattagagt tgatgaagaa
    11161 tctcttgata gacaattgaa acttgctgga tgtgaagaga gaaaagagtt tgactatcat
    11221 aagatgcttt taaatggcga acttccgtat acaattggtg gaggaattgg tcagtcaaga
    11281 atatgtatgt atttcttaca aaaagctcat ataggagaag tacaagttgg agtatggcca
    11341 caggatatga ttcaaaattg tagtagtgca ggaattgaat tattataatt taaatataaa
    11401 ctaattgata aaatttatat ctaatatgtt catcaaatta attatatcgg ttataatgta
    11461 gaataaccta taattataag aggtgataaa tatggagttt ttaaaaataa tcattaatat
    11521 agttttagat attttaaaaa aaattttggt cagatttaaa aatgctaaat ttggtttggt
    11581 ttttgtattt gatttactta aactacctga ttttatgaca gataaaagaa tcaatatagt
    11641 tgataaaata aaggtaattt ctgtattaat ttttactata tcctactttg tatctggggt
    11701 tgatataatt ccagagatga ttgcaggggc atttggtttt atagatgatg ctatagtttt
    11761 aatttggagt ataggtatag tgaatgaaga aattaataag tatagagtta tcgcaaaaaa
    11821 agataaacat tcaaatataa ttgaaaatgt agaattttct ataaaagatg aagaagaata
    11881 acaatttata tatgttattc ttttttttat taacaaaaaa tggttaaatt gttgacggag
    11941 aggtaaaact ctattatcat aataagtgga tatccaatat ttaactaagt attcaatgtg
    12001 caagaatgct taatttaaaa aagttatgga gggtaaatat tatgaagaaa aaaataggtt
    12061 tagtgcccaa actaatcatc ggtatcatag tcggtatttt aatcggaagt tatgcaccag
    12121 aaataatagt acaaatatta gtaacagtta gtactttatt ctcagcattc ctaaaatttg
    12181 ttataccatt tataattata ggttttgtaa cagcaggtat agctgattta gcaacaggtg
    12241 caggaaagtt attggggata actacgggaa tagcatatgg ttctacctta atagcaggat
    12301 tattatcatt tgcagtatct acattaatct tcccaaattt tattgatgca agtgttgcaa
    12361 gtcaaattgg agacccagag gcaggaatgt tagctccaat attcacaatt ccattagagc
    12421 ctatggtaga tgtaacagct gcaatcgtat ttgcatttgt aatgggactt ggaatatcag
    12481 ctttaagaaa tcatggaaaa ggagagactt tatttaattt cttccaagaa tttcaaggaa
    12541 tcgtaacgaa aacgttgtca acagtgatta tacctttatt acctttatac atagcaggaa
    12601 cttttgcaaa tataactata gcaggggaag tatggacaat attaggggta ttctggaaag
    12661 tatacttagt agtaatacct ttacattttg tatatatggc atgtcaattc acagcagcag
    12721 gtgtattttc aggaaaaaat ccagtaagaa tgttaaaaaa tcaagtacct ggatatttaa
    12781 cagctatagg aactcaatct tcagcagcaa ctataccagt taatgttggt tgtgcagaaa
    12841 aaaatggagt aagtaagcaa ataagagaat ttgttgtacc tctttgtgca acaatacatt
    12901 tatcaggtag tatgatatca ataacaggat ttacaactgc agttttaatg atgaatggta
    12961 tgccacatgg aataggtgta ttattccctt atattgcaat gttaggtata gcaatggttg
    13021 cagcaccagg agcaccaggt ggggctataa tgagtgcact tcctttccta ccaatggttg
    13081 gaataccatc agatggtggg cttgcaagtt taatgatagc tttatactta actcaagaca
    13141 gttttggtac agcatgtaat gtatcaggag ataatgcaat agcagtagta gtagataaaa
    13201 taaatgatgg aatgagtaaa aaaggaaata aagaagagca aagtgcttag ttttttaata
    13261 atcaaaaatg agttatctca aatagagata gctcattttt tatggtataa aaaattataa
    13321 aataaagtga tttttttatt taaaaattgc tttaagagat gatattaata atttattttc
    13381 attaggtttt aatatacata ctctaaagaa atactcatct agaccttcaa atgaacaaca
    13441 atctcttatt attatttttt gtggaagcaa ttgttctcga agagatttag cagtcaattc
    13501 ctttgtcttg attttacaca atatgaaatt gcctttagta ttgtatatat ctaagctttt
    13561 tatattctta agctctttaa gcaggtaatc tctttcagtg ttcattaagg aaatagtatt
    13621 tgatataaaa tctaaatcac taaacatgat ttcacccatt ttagaagcta ttatgtttat
    13681 attccataaa tctagatttt tatttatttc atttttgaca ttagtgttag atataagacc
    13741 atatccaagc cttattccag gcgttgaaaa aaattttgat gtacctctta taacgaataa
    13801 attagaataa tcatcaacta attgagtaca agagtatgta tctgtatcag taaactctac
    13861 ataagtctca tcaatcatta aaaatgagtc tgtatttttt aaaatttctc tcacttctac
    13921 tttagtaaat gcaaatccag ttggattact aggattacag attactacca aatcataatc
    13981 tttagcattg atagttttta taagattttc taaatttata tgaaaattat cttcttcttt
    14041 tgcaaagtat ttttctatag aacaattaat tttagataac tctttttcat actctgaata
    14101 ggctggagag agtaaaagag cctgtttagg gttaattgta tgtataaatg atgaaattag
    14161 ttctgtagct ccactgccaa gtactatatt atctatagaa catttgcagt aattggatat
    14221 agaagttttt aagtctatgt aatcagggtc aggatacata gataccatat ctatattgtt
    14281 tacaatgtat tgtttggcta aacttgatgt accaaaagga tttatatttg agctaaaatc
    14341 cataaattct tcctttgaaa agccatactt actagataaa ctatataaat tagctccatg
    14401 gtttgtgctc ataaaagtac ctccaaaaat aaaattatat aatcgataaa aataactgat
    14461 taatatatgt acatatagat tataatacaa aggaatcgtt attccaaatt tttaatggaa
    14521 aaagcaaaaa agtatggata agctgttaat ttaggtttaa aaatgatgaa aaagatttta
    14581 attgatattt tggtatgcta taatatataa gttaatagaa ggaaagcggg aggatacaaa
    14641 atgtacaaag ttacaggaaa aatgttgaca gaagaacaaa taaaagaaaa agtttatgaa
    14701 ttaggaaaaa agatagaaga agactttaaa ggcgaagatt tactagtggt aggtatactt
    14761 aaaggagcta gtgtatttgt ttcagattta ataagatgta tagatttgga tgtaaatata
    14821 gattttatga gtgttacaag ttatggaaac tcaacagagt cttctggaac agttaaaata
    14881 ttaaaagatt tagatgtaga tattgaagga aagaatgtgt tgattgtaga ggatataata
    14941 gattctggtt taacattaag taatctagtt gcagctttaa aaactagaaa tccaaaatct
    15001 ttaaaactat gtactctact tgacaaacct cagagaagaa aagctaatat acctgtagac
    15061 tatgttggat ttgttataga agacaaattt attgttggtt atggaataga ctatgctgaa
    15121 aagtatagaa atttacctta tataggtata gttgaggatg tagaatagat ttaaatgtga
    15181 atcaaaagca ctaatttcta agggagttag tgctttttag ttaattatat aaaattttag
    15241 taaaggagac atctatatgg atattcagtt aaatatatta ttatttttcc aaaatttaag
    15301 aaatccaatt ttgaacttta tatttcttgt ctttacaata agcacagaag caccgttatt
    15361 aatattaatt acaacaatta tatactggtg tataaataaa aaatgtggac agaaaatact
    15421 gtttgctata attggaaatt ttgttgtaaa tttaggcatc aaagagtttg taaaagctcc
    15481 cagaccaata ggaataaaag gtcttgaatc attgagagtt tctactgctg gtggttattc
    15541 ttttccaagt gctcatactc aaactgcaac aacattttgg gttagtatta tgactatatt
    15601 taaaaagaga tatcttcatt taataggtgc agttatggtt ttaggagtag gattgtctag
    15661 gctgtattta gctgttcatt ggccaataga tgtgatagcg ggttggatat taggaatatt
    15721 ttttacagtt gtatttgtaa aaatatttga ctatattgat gataataaaa attatatttt
    15781 acttgtagtt ttacttatac catttataat agtggctata tttctaaata gccctgaata
    15841 ttttgaaaaa tttggtataa tagtaggatt tgtatttgga tatatggttg aggatagatt
    15901 tgttaaattt aatacagata ataataacaa aaaaataaat tttagtaata aaaataaggg
    15961 gagtaaaaat ataatttttt caagaatatg taggtttttg gttgggatag taacaatagg
    16021 aatgttatat attggcatta agttgtttat ttcaatgtta atagtcactt taaatataag
    16081 tgactctact atgagtataa tttttatgaa ttttattaag aatacagtag ttgtttttta
    16141 tggaatagca ggagctccag cattatttaa acttttaaaa ttaaattgat tttaaggtat
    16201 agagtcaata gtttaaatta aaaaaattaa gggtagaaaa gagtgattat ttgaagaagg
    16261 tagtaataat aggtggtgga ccagcaggga tgatagccgc atcaactgca tgtgagaaag
    16321 gctatgatgt aacattaatt gagaaaaatc ataaattagg aaaaaaactt gcaataacag
    16381 gaaaaggtag atgtaatatt actaacgctt gtgaaataga ggaattaata gaaaatgtac
    16441 ctacaaatgg aaaatttctt tatagtgctt tttatacttt tacaaatgat gatgtaataa
    16501 gtatgtttaa taatcttgga gtaaagacta aaacagaaag aggaaaaagg gtttttccag
    16561 agagtgataa agcatttgat attgtaaatg ctcttgaaag acaacttaaa agtaagaaag
    16621 taaatatatt attaaattcg aaagtagaaa aaataatatc taaaaataat aaaattgaaa
    16681 aagtaatttt aaacgataaa aaagaaatta agtgtgattc agttgtagta gcaactggtg
    16741 gattaagtta tccacttact ggttctactg gtgatggata taaatttgca atttcacagg
    16801 ggcacacaat tatagataca aaaccatctt taattggtat agaagtacaa gaaagtttta
    16861 caaaagattt agaaaaatta tcattgagaa atgttgaaat aagggtgttt aatagcaaac
    16921 aaaagaaagt ttatagtgat tttggagaac ttgaatttac taaatttggt ttagatggac
    16981 ctataatcaa gtctgcaagt tgtagaatga aagatacaag gaaagaaaat tacactatat
    17041 tattagattt aaaacctgca ttagatgagg aaaaattgga taaaagagtt caaaaagatt
    17101 ttcagaagta tacaaataaa aaatttgaaa aagcattaga tgatttactt ccaaaaaaac
    17161 taatacctat aataataaac ttaagtgaaa ttaatccaaa tacggttgtt caccaaattt
    17221 ctagagagca gagaaaaaac ttagtccatt tattaaaaaa tttaaagttt acagtaaaga
    17281 gatacagacc tattgaagaa gctataataa cttctggtgg tgtaaaagta aacgaaataa
    17341 attcaagtac aatggaatca aaattagtag aaggtttatt ttttgctgga gaagtaatag
    17401 atatagatgc atatacaggt ggattcaatt tgcaaatagc tttttctact ggatatttgg
    17461 caggttttaa ttgctaaaaa ttacctgaat taaaaatgtt taaaatcaca taatattatc
    17521 agcaacacaa aataaattaa acagaaggaa ggtaatcaac atggcaagta acaataacaa
    17581 caacagaaca gtagtaccag gagcaaaaga ggctttaaac caaatgaaat tagaaatagc
    17641 taatgaactt ggtatgagta attatcaaca agttgataaa ggaaacctta ctgcaagaga
    17701 aaatggttat gtaggtggat acatgactaa aaaattagtt gaaatggctg aacaacaaat
    17761 ggctggaaaa agcaacagat agttataaat aattatttat aactattggc tattagttta
    17821 gctagagcta tctcaaatat agaaagtaat ttctaaattg agatagcttt tttttattaa
    17881 attattaaat agatatattt ttatgtaata aattttatgt atttggataa aataatacaa
    17941 atgaatatgt tacataagga gtgatgtaaa atgagtagtg taaattttga ggatttaaaa
    18001 aacaaattta ttaatagtga cttagatgaa aaaataagaa tatatacaac aacagaaggt
    18061 ttatcagtag aacaatttaa agagctacta aaatattatc caatacaaca tttatctaaa
    18121 ctagaaaaag cacttggata gcagtattta atccaaaatt aaaaaggcaa tttatttaat
    18181 attaaaaata ttttttatta aataaattgc ctttttataa taggtttaaa ttattatgct
    18241 tttgatatat aattatagat tctctcattt ccaagttcaa tagacccagt attaataaga
    18301 gtaactattc cttctatatc tctagcagtt aagtatttat atggaattaa taaattataa
    18361 ccagtattac ttagtaagtt ttttaattgg gttatttgag agatattgaa acattttgca
    18421 gttttagtag atttttttct taagttagat aaattttcat tttgaatatt tattaaagtt
    18481 tctatatagt ttctataaga tatcttaaag tttgtctcat tgactatttt ttcattaagt
    18541 atactactga taaaatcttg agacaatata ttatctgcaa atttatctgc tgttaatttt
    18601 ccagatgtta tgcctttttg atattttcta agaatttttt tagtatagca gtatatttta
    18661 tttctcgttt ctaaatcgag ttgttctatt tgcacaaaac cacttccttc taattataaa
    18721 aatatttatt aaaaatctat acttaactaa taaagcttca ataacttatt tacaataaat
    18781 actaaatcat aaaatatttg ttatttttta ttattaccaa gtataaaaaa tattataatt
    18841 gtaaaactta caaatatatg taaaatattt aggagtgatg aaaatgttaa atgggataat
    18901 aaagcttttt tattctgaaa ttgtaaaaac aaagattaat aacttagatt caagtggata
    18961 ttctttaaga gatgaatgta aagaaatcag aaatataact ctaaagatag atagtgtatt
    19021 ttttaagctt ttaaagttag cttttggttc aatattgctt atggtagctt ttaatttaaa
    19081 catggtttta ggtataggag tgtttttagt tggtgtagtt tatatactat ataaaagaaa
    19141 tatggaagaa caagtaaaag aacatataaa taatgtgaaa aataatatcg acatctctaa
    19201 aataactttt atggatgaaa aagggagaac aggtctaaat gcattaatta cattacttct
    19261 tataggatta ttgacaggat ttaactggat attagttgct agttttgtcg ttgtttttgt
    19321 ttttactata aagaatgtat gttaaaatac taataaatag tttaatatat agggggagaa
    19381 attgagtatg actaatggca gttataaaat gaagaattta gtggaaatat ccgctatggt
    19441 gactgaatct acagattttt ttaatataaa agatgacata atagaaaaaa tgttagaagt
    19501 tgttcaccct acaaaggcat gtgtgaatct attttataaa aatgattcta aatatgccta
    19561 cttagtatgt tcgcaaacct tagaatatgt acctcaaatt tttccaataa atagtcttaa
    19621 aggtgcaaaa attgattttg acacatatcc agagtatata catgaggctg taaatgaaaa
    19681 aaagataata tatgtggaaa atgtatttga agattctaga gcagaaggtg aacgagattt
    19741 agcaaaagct gaaggttata ttgggagaat agtttttcct tttataataa ataacgtagt
    19801 tgttggattt atgacttgtt ttttaaaaga ggatgattat ataacagaac aagatataga
    19861 ttttatatca tctgttgcat cactcttaag tttatcaata gaaataacta ataaaaataa
    19921 taatgtacaa attttaattg ataaactaag aggctcaata agtgccataa atgaagctac
    19981 taaaaaacta taccttaata agagtattga tggctttttg gaaaatttaa gtaaacaagc
    20041 gtgtaatatt actaatagta aagaagcttt aataattata aatagtcatc ataataagga
    20101 tgctatagtt agttgttata atgttgagca aaaaaagaaa acaaatttat atcctatgat
    20161 tgatatgttt ttaaagaaag agtcattggg ggggtatcta aataatttaa aaccatgctc
    20221 aaaaaaggga ataaatttgg aaagttatat atattacaaa cttcaggata aaaatgatac
    20281 tataggatgt atagtttgtg caaatagcaa aaactatact agtgatgatt taaatattct
    20341 tagtatattg tccaaacaag tttctgttgg tatgcaatta tatgaacata atgaaaagga
    20401 agtaaaacac aaagttcttg aaaacgagtt gaacatatta aataaacaac aacagcttat
    20461 tatggataaa ggtaaaatgg attgtaatga tgagaaagaa ctttattttt accataaacc
    20521 agcaagggta gtaggtggag atttttatta tgctacaaaa gtggatgatg aacatatagt
    20581 ttatattgtt gctgatgtaa tgggacatgg aatggtatca aattatgttg ttgcaatgat
    20641 aaaaggtgct tttaaagtat tatgtaatca atacaataca ccaagagaaa taatgacaaa
    20701 tttaaatagg atgttatatg atgaatttga caagatggga gtatttacaa cttgtttgat
    20761 aggtattata gatactaaag aaaatatgat tactgtgtct aatgcaggtc actatagtcc
    20821 tgtagttgta aagaaagatg gcacaataga aaataattta aattgtaaaa agggcatacc
    20881 tattggaata atggaagatg ctacttatga aaataatact ctttcaatgg aagattatgc
    20941 aatggtatgt atgtacactg atggaatttt agagataaaa aattcatcaa aagaagagta
    21001 tggtataaac cgattagaaa actttctaaa agaaaatttt agattaaatc aacagttaat
    21061 agtagaaaat ctaaaattgg atttaaagaa cttctcatca aaagataatt atgatgatga
    21121 tattttaata gttatgttaa aggataggta gattgattat ggattcaaac gataaaaaag
    21181 gacttaaaag tccagagatt ataataataa atggaagtcc aagaattaat aaaaattgca
    21241 gttttatagc aaatgaagta aaagctatgc tagaagagtg tggattaagt tgtaaaatat
    21301 ttaatatcta caatatggat attgaatatt gcacagcatg tggattttgt gaaaaaacag
    21361 ggtattgcaa gtttaatgat gatatgaagc cattatataa aatgtttgat gattcaaaag
    21421 gaactatagt aataagtcct gtacattttg attcaatatc agctaaatta aagactgttg
    21481 ttgatagaac tcaagctata tatgcaagta aatatgtatt aaataaacct tcaattgata
    21541 gaagtaagaa acggatagga atgtatattg cagtgggagg gagtaaatca tatgattcac
    21601 agtttaaagg tggacagatt gttatggatt ttttctttaa gagtataaat actaaacttt
    21661 tacacaatat atatttaaac aatacagatg atgtatctta tatagataat caagaattta
    21721 aagatataat atataaaaat ttaaagatat atattgattc aatataattt gattttgtag
    21781 gtaatattat gtataattgt attgcaataa tttaaataat taaaaggagg gaatttcaat
    21841 gaaattacca atagcattat ctaataaaca tgttcatttg agtcaagctg atatagacgt
    21901 attatttgga gcaggtcatg agttaactaa atttaaacca ttatctcaac cagggcaata
    21961 tgcctgtgaa gaaaaagtgg atttagtagg accaaaagga actttaaaag gagttagagt
    22021 actaggacca gctagagctc atactcaagt tgaaatatct ttagcagatg gtttcccatt
    22081 aggagtaaaa gcaccaataa aagaatctgg tgatatagct ggaactccag gtgttaaatt
    22141 agtaggacct gctggagaag ttgaaatgaa agaaggagtt atagttgctt ctagacatat
    22201 acatatgagc aatgaagatg ctgctaaatt tggtgtaact gataagcaaa tagtaaaagt
    22261 taaaacatct ggaccaagag cattagtatt tgaaaatgta ttagtaagag ctagtgacag
    22321 ctttaatcta gaaatgcatg ttgatatgga agagggaaat gcagcaggcg ttaaaaatgg
    22381 agatttagta gaattaatag ttgattaata ttataaatag actttatgct agtagatatt
    22441 ttatttgcta gcatattttt aaatgtaata aatttctata ggataaattt aatcgattag
    22501 attaatttat aaaataaagg ggtattataa aaaatgtaat aaaaacttac aaaaatatg
//
LOCUS       DAEOSP010000045        22500 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.045, whole genome shotgun sequence.
ACCESSION   DAEOSP010000045 DAEOSP010000000
VERSION     DAEOSP010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22500
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.045"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            121..1635
                     /gene="glpK"
                     /locus_tag="KRH54_001417"
     CDS             121..1635
                     /gene="glpK"
                     /locus_tag="KRH54_001417"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBG8579084.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            complement(1749..2378)
                     /locus_tag="KRH54_001418"
     CDS             complement(1749..2378)
                     /locus_tag="KRH54_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8579085.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            2966..3757
                     /locus_tag="KRH54_001419"
     CDS             2966..3757
                     /locus_tag="KRH54_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBG8579086.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            3786..5006
                     /locus_tag="KRH54_001420"
     CDS             3786..5006
                     /locus_tag="KRH54_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBG8579087.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            5026..5721
                     /gene="pxpB"
                     /locus_tag="KRH54_001421"
     CDS             5026..5721
                     /gene="pxpB"
                     /locus_tag="KRH54_001421"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBG8579088.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            5751..6737
                     /locus_tag="KRH54_001422"
     CDS             5751..6737
                     /locus_tag="KRH54_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBG8579089.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(6932..7498)
                     /locus_tag="KRH54_001423"
     CDS             complement(6932..7498)
                     /locus_tag="KRH54_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8579090.1"
                     /translation="MLGEKMRNIKKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            7850..8917
                     /locus_tag="KRH54_001424"
     CDS             7850..8917
                     /locus_tag="KRH54_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBG8579091.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            8936..10096
                     /locus_tag="KRH54_001425"
     CDS             8936..10096
                     /locus_tag="KRH54_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG8579092.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPISMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            10109..12205
                     /locus_tag="KRH54_001426"
     CDS             10109..12205
                     /locus_tag="KRH54_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBG8579093.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSAKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(12364..12708)
                     /locus_tag="KRH54_001427"
     CDS             complement(12364..12708)
                     /locus_tag="KRH54_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG8579094.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            13000..13890
                     /locus_tag="KRH54_001428"
     CDS             13000..13890
                     /locus_tag="KRH54_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG8579095.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            14108..15394
                     /locus_tag="KRH54_001429"
     CDS             14108..15394
                     /locus_tag="KRH54_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG8579096.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(15483..15926)
                     /locus_tag="KRH54_001430"
     CDS             complement(15483..15926)
                     /locus_tag="KRH54_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBG8579097.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(15950..16744)
                     /locus_tag="KRH54_001431"
     CDS             complement(15950..16744)
                     /locus_tag="KRH54_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBG8579098.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(17310..17786)
                     /locus_tag="KRH54_001432"
     CDS             complement(17310..17786)
                     /locus_tag="KRH54_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG8579099.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(17887..19056)
                     /locus_tag="KRH54_001433"
     CDS             complement(17887..19056)
                     /locus_tag="KRH54_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8579100.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLEKPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTNTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            19219..20082
                     /locus_tag="KRH54_001434"
     CDS             19219..20082
                     /locus_tag="KRH54_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBG8579101.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWGKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            20153..20746
                     /locus_tag="KRH54_001435"
     CDS             20153..20746
                     /locus_tag="KRH54_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBG8579102.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(20997..21632)
                     /locus_tag="KRH54_001436"
     CDS             complement(20997..21632)
                     /locus_tag="KRH54_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG8579103.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(21712..22443)
                     /locus_tag="KRH54_001437"
     CDS             complement(21712..22443)
                     /locus_tag="KRH54_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG8579104.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LTLNIELKTDKINYPNIEKGVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
BASE COUNT         8545 a         2626 c         3795 g         7534 t
ORIGIN      
        1 cttcttacta aatatctatt atattaaatt tgtacttaag tatgattatt tattgaaata
       61 tgtgatatta aatttttctt tttaataaat taaatattat atgataagga gaatatggtg
      121 atggaaaaga agtatgtaat ggcattagac caaggaacaa ccagttcaag agctatacta
      181 tttaataaaa aaggcgaaat agtgaaaatt gcacaaaaag agttcaatca aatttatcca
      241 aaagctggat gggtagagca tgaccctatg gaaatatggg ggtcacaaag tggggtaatg
      301 agagaagtta tagaaacagc aggtataaga ccagaagaaa ttgcttcaat agggataact
      361 aatcaaagag agactacggt tgtttggagt aggtatactg gtaaacctat ttataacgca
      421 atagtatggc agtgtaggag aacgtcagaa atatgtgatg aacttaaaaa taaaggattg
      481 gaagaatcaa taaaagaaaa aacaggtctt ttaatagatg catacttttc agcaacaaaa
      541 gtaaaatgga tattagataa tgtagaagga gctagggaaa aagcagaaca aggagagtta
      601 ttatttggaa ctatagatac atggttaata tggaatttaa ctcgtggaaa agtacatgta
      661 acagattact caaatgcatc tagaacaatg atgtacaata taaatgattt agaatgggat
      721 gaggatatat tacgagaatt ggacatacca ataagcatgt tgccattagt aaaaccttct
      781 agctatgttt atggtcatac agatgagcgc atgttatcag gagctaaaat acctattgcg
      841 ggatgtgctg gagaccaaca agcagcatta tttggtcaaa attgtgttga agaaggtact
      901 gcaaaaaata cttatggaac gggttgtttc ttgcttatga atacaggcag taacatagtt
      961 aaatctaagc atgggttact tacaacaatt gcttggggag ttgatggaaa agtaacatat
     1021 gcattagaag gaagtatatt tattggagga gcatctgttc aatggcttag agatgaatta
     1081 aagataattg aatctgcaaa agatagtgaa atgtatgcaa atagggtaga ggatactaat
     1141 ggtgtatatg tagtgccagc atttacaggt cttggagctc catattggga tatgtatgct
     1201 aggggttcaa ttttaggact tacaagagga gcaaaaaaag aacatatagt aagagccaca
     1261 ttggaatcaa ttgcttatca gacaaaggat gttttagaag caatgcaaaa cgattcaaaa
     1321 ttaaaactaa aatcattaaa agttgatggt ggagcaagta ataataattt tttaatgcaa
     1381 tttcaatcag atatattaaa tgtagatata gatagaccca aaatagtaga aactacagct
     1441 ttaggagctg cttatttagc agggttgtct gttggattct atattggaag aaatgaaatt
     1501 acttcaaagt ggtcagtaga aaaagagttt aatccaaata tgagtgaaga gaagagatgt
     1561 aaattatata aaggatggaa aaaagctgta tcgagagctt taagctggga aaaagaagat
     1621 gaattggata tttaaaaatt aatcaaagtt aaatttattt gaatttaaaa agagctttat
     1681 gtaaaatatg aagctctttt tttgttgtta atcttgcatt tttatataat cacactaaat
     1741 tatataattt atttttcgtt ttcatataaa aacattggta tagaattgta aatgtgttgg
     1801 tcaccaacta ctaagagaat gtctccttct aaaaattcaa tatatggtcc aggagatatt
     1861 ataagttctt cacctctttt aattgcaact attgtagcac ctgtattttg ccaaaattta
     1921 gtttgtgcag ctgtttgacc aatatatttg caattagaat ttatggtgaa ctgaacgggc
     1981 actaaaggat tgattatttc aagtctgtta gaatagtcta aaattttatt tatagtatct
     2041 aatatttcat tttctaaatt agatttagta tctattaaat tataaagtgt tgatttatat
     2101 gatgatatat ttgtaatatc tctatatttt tttataaaag aagaagcttt gtctggagat
     2161 agtacaataa cacctttacc ctttatgctt ttaacaacgc caatgtcctc taaaattttg
     2221 acagctcttc ttatcgtttc tggagataca ttgtatttac cagccaaaac agagcgtcca
     2281 aatagtactg aatcctcttg tatttcaccc gtgtatattt tatttgcaat atccaacgct
     2341 attttttggt atattggcat cgtatagttt ttatccattg tttttccccc aatacaaaat
     2401 ttatgttaaa aaaatatata atcttaacaa tactattgta taatttatag gtttatatgt
     2461 ctacaatatg tacaaatatt gtgcatgttt taaaataata ttttaacatg catttagtat
     2521 acatgatttt attatactta aaactatata aagaattaat tataaaaata taaagatatt
     2581 ttacttacaa agaatgataa ttatatagat gaaagaaaca aatgaatgaa gaaaaatgat
     2641 tctgtcttgc atataaagtg attaaaaatg aaaaatactt taaaaatgaa ggttaaagcc
     2701 cttattttaa gtgaaaataa ttaattatta actaaaaaat ggtaaaaaga ttttttgaaa
     2761 aagaaataca aatgaatgtt caattaaaat aattgcaaaa atattcctta aatggtaaaa
     2821 ttatattaat taaatgcaat agaatagttg caaaaataaa attgaataca agtgataaaa
     2881 tatttcatgc aatatataaa ataaacaaaa tttaaaaatt aaaactactt attctatcaa
     2941 aatgttagcg aggaggggtc ataaattgta taaagtagat ttaaatagtg atttaggaga
     3001 aagttttgga acgtataaga ttggtttaga tgaagaagtt ttaaaatata tttcatcagc
     3061 caatatagct tgtgggtttc atgcaggtga ccctagtcat atggaaaaaa cagtacagct
     3121 agctaaaaaa aatggagtga aaataggcgc acatccaggt ttcctagatt taataggatt
     3181 tggaagacgt gagatgaaaa taacaaagca ggaagctaaa gattatacaa aatatcaact
     3241 gggtgcttta atggcatttg caagttcaaa tggatgtaat atacaacatg taaaaccaca
     3301 tggagcatta tacaatatgg cagctaaaga taaagagtta gctatgggga tttgtgaagc
     3361 tatatatgaa gtcgataaag atattatact gttggggcta tataatagtg aaatgataaa
     3421 ttctgctaaa gaaattggac ttaggtttgc aaatgaagta tttgcagata gagcttatga
     3481 taataatggt tttttggttc caagaaatgt agaaggagca gttatacatg atacaaaaca
     3541 tgcaatagat agggttgtta ggatggtaaa agaaggaact gtagaaactt taacaggtga
     3601 agttatacat atcaaagcag attctatatg tgtacatggg gacaatccaa aagcaataga
     3661 gtttgtcaaa gaaataagaa agcgttttga attagaaggt atagaagttt gtccattaga
     3721 aaacatagaa gtttgttcgt cagagaacat agtataagac tgataacatg agggggagat
     3781 aaaatatgag tacaaaaaat attacagata aaactaaaaa taaaaaagat gtcggagcac
     3841 ttataggggc tgcatttata atggcaacat cagcaattgg accagggttt ttaactcaga
     3901 ctgcacaatt tacccaagat tttggaccga atttttcatt tgtaatttta attacaacaa
     3961 tcctttttat aggggctcaa gtaaatgtat ggagagtaat tggtgtatca ggacttagag
     4021 gtcaggatat agcaaataaa ataatacctg gattaggcta tttagtagct tttttagtag
     4081 ctcttggagg attagctttt aatataggaa atgttggtgg agctgcactt ggtatgaatg
     4141 taatgtttaa tatgaatatg acattaggaa ctgtattaag tggattaata gctatatttg
     4201 tatttatgtc aaaaaattca aattctctag tcgataagat aactaaattt ttagcattag
     4261 gaatgataat tatagttgga tatgtagcaa tatcaaatca tcctcctgtt ggagaggcag
     4321 tatcaagaat ggtaaaacca gaaaatccaa aaggactgat attcccaata ataactttac
     4381 ttggaggaag tgttggaggt tatataactt ttgctggagg acatagatta atagacggtg
     4441 gaataacagg agaagaaaat ataaaagaaa taactaaatc ttctcttttg ggtatattag
     4501 ttgccacaat gatgagagtt cttttatttt tagcaatact ggcagtggtt tcaaaaggtc
     4561 ttcaacttga cccagaaaat ccagcagctt cagcttttaa atttagtgca ggagcaatag
     4621 gatataaatt ctttggactt gtattatggt ctgctgcaat tacatcagtt ataggagctg
     4681 cttatacatc agtttctttc ttaaagacac taaatccttt tatagataaa tatgaaaaat
     4741 attttataat agcatttata gcaatatcaa cacttataat ggcatttata ggaaaaccag
     4801 ctactttatt aattttagca ggagctttaa atggattgat acttccaatc acattaggaa
     4861 ttatgcttat agcatctaaa agaaaagata ttgttggaga ttacaagcat cctacatggc
     4921 ttttaatatt tggattaata gtagttttaa tatctgcata tacaggaata acttctctta
     4981 gcagcctagg tgctttattt gcttaattta gaagaggtga catttatgga aactagatac
     5041 ttgatatcag gggataaggc agtggttgct gaatttggaa atgagatatc agaagatata
     5101 aacaaaaaag taatttcatt tatgagagca atagaaatat cgaatcttaa aggtattgta
     5161 acagaaatgg taccaacata taggtcactt atgataagtt ataatccatt agaaatagat
     5221 tttgatagtt tgattgagaa tttaaaaaaa atagaagata atttggaaag tatagtatta
     5281 ccaaaaccta aaatacatga gattccagta tgttatgatg aggtttttgg tatagatatt
     5341 aaaaacgttg caagctacaa taatctcact gtagatgaag ttataaaaat acacacaagc
     5401 agagagtact tgatatatat gctaggtttt actcctggat ttccatatct aggtggtatg
     5461 gatgaaagaa tagctacacc tagattagaa gtgccaagga ctaaaattta tggtgggagt
     5521 gttggaatag caggttccca gactggtgta tatcctattg atagtcctgg tggatggcaa
     5581 ataattggta ggacaccttt aaaattatat gatgaaaata gagaagaaca gattttgctt
     5641 agagctggag attttataaa atttgttcca ataacattag atgaatttat agaaatcgaa
     5701 aaaaataact taagtattta aatatcgtta tagtacaggg agtggtatat atgggattca
     5761 aagttgaatt aggaggtttc caaacattaa tacaagacag aggtagagtt ggctatggtc
     5821 aatatggtgt atctggatgt ggagcaatgg atgagtatgc acatagagta ggaaatatat
     5881 tagtaggaaa ttctgaagat gaggcttctt tagaagtttt aatgttaggt cctacaatta
     5941 cctttgatga atatacgcaa atagcagtaa ctgggggaga tttaggggct aaaataaatg
     6001 gaaaagaaat tcaaaactgg aggtcatatc aaataaatcc aggtgatgta ttatcattta
     6061 gaggtgtgaa atcaggtgct agagcctatg tgtcaatagc tggtggaata gatgttccac
     6121 ttgcaatggg aagtaagtca acttatacaa gagctaaaat tggtggattt gaaggtagag
     6181 cacttaaaaa aggagattat ataaatactt ttattcagga aaaagatttt acaataaata
     6241 aaaaattaag ttcaaaatat attccaactt atagttcaga aatcgtacta agaattgtaa
     6301 aaggtcctca atttgatgct ttttctaacg gtgaagtaga gaaattttta tcaaataaat
     6361 acaaagttac gaatgaaata gatagaatgg gttgtagact agatggagag agtataaagc
     6421 accttaatgg agcagatatt atatctgatg gtatttctta tggagctata caagtacctg
     6481 gtcatggaaa accgattata atgttatcag atagacagac tagtggtggc tatactaaga
     6541 tagggaacgt tataagtgta gatttgtata agttagcaca agcaaagcca aatgatgtag
     6601 taaaatttga acttgtagat atatatgaag cacatagact attgagagag caagaagata
     6661 agattcaaga tatctacaag agtatgaaaa atataagagt tgtaaaggca aaagtattaa
     6721 atgatatagc agtttaattt taacaaatac aatttttatt ataatttata atataactta
     6781 caaatagcta tttactattt accataatta agatggttta gattagtcaa atagctattt
     6841 ttttagtttg agtaaatatt ttaaatttaa aataaaagca atatttaaat ctattatatt
     6901 atctacaaaa agtaggtgtg tcatattgag tttatttaga ttttattggc caaattggag
     6961 gtgatataac tccatatcca cgagctattg tatctttact tggattgatg attttatgtg
     7021 gtacattagc ttttataaat gttgagtcac ctggatttaa tctaactacc ttttcaccca
     7081 tacaaatatc aaccacacca ctcgttacca ccataatttc ttctgatgca tgatatatat
     7141 aatctttggc atcctcactt tctggtttaa gttcaaactc tacaaataaa attgaaggtg
     7201 taaactcagt tgtaggcatt ggagatacta tctcgtaaaa catttcactc tttggaaatt
     7261 tcatcataac tctatcttct tttctaatga ctaaatcatc agtattattg tcatccataa
     7321 acatatataa aggagtgtct aaaacttcag cgatttttct caatgaagac aaggatggtt
     7381 caatagcatc tctttctatt tgagagatat aacttataga taaatctgtc aatttggaaa
     7441 catcacttaa tgtttttttc ttgctttttt ttatatttct catcttttca cctagcataa
     7501 gaacctccaa ataataaaat taatataaaa tattcgctta aaacaaagta attatagcac
     7561 aacatagaag tagtggcaat aagaatacat agtagatttt gacaattaaa atttaattaa
     7621 atccaaataa aagtctatga aaatgaatta ttgttaaatt aatacaaaga aaataataat
     7681 aaaaaaattt aatagaagag tattaattga attaagatta tcacattata ttttggaaaa
     7741 gagtaaattt tcagtaaatt atcttgacat atatattgaa gtaataatat aatttaaagt
     7801 ataaaatttt aaaaattgaa ttataacttc aaaaaataag gagggtaata tgaagataac
     7861 agatatcaaa tttgaaaagc ttagaattaa attaaaaaaa cctgtagtag tttcatttgg
     7921 agtaattgag tatggagaaa gtataatttt aaaaattgaa actgatgaag ggtattgtgg
     7981 atttggcgaa gcagcacctc ttgcagcagt tacaggtgaa gttttggata atgttctttc
     8041 agtattgctt atgtttaaaa aagagttaat aggcaaagac ccactagata ttgaaaccat
     8101 acataccatc atggatggaa ttattatagg taatacttct gcaaaagcag ctattgacat
     8161 agctttatat gatattaaag gaaaaattat gaatgcacct ctttataaag ttcttggagg
     8221 atttgatagt aaagtccaaa cggatattac tataagtata gataagccag aaaaaatggc
     8281 agtagaagct ttagaaagag taaaagaagg ttttaggata ttgaagctta aagcaggtat
     8341 aaatcctgaa gacgatattg aagctgtaaa gttaattaga gaagccgttg gagagagtat
     8401 aagaattaga attgatgcaa atcaaggatg gaacgtaaat agcagtataa atactatcaa
     8461 aaagttagaa gaatttaatg ttgatgcaat tgaacaagca cttccacatt gggatttaga
     8521 tggtactgct tacataagaa ataaaagtaa cacaaaaatt atgatagatg aatcattaca
     8581 ttcaccaata gatgcaatta aggctataaa aaagaatgca gttgatacat ttaatattaa
     8641 gctgatgaaa tcaagtggaa tatatcctgc tataaaaatc aacaacatag ctgaggcaag
     8701 tggagtaaat tgtatgttag gatgtatgct ggaaacgaga attggtataa cagcagcagc
     8761 aaatttaata gcatctaaaa agaacataac agaagctgac cttgatagtt ttatgttttg
     8821 tgaagaatta gaaggtatat ctggaggatt tgtaatggat ggagatatta tgaatcttgt
     8881 caataaacca ggtttaggaa tagaagtaaa cttatgataa atttggaggt taaatatgaa
     8941 tacacttttt tcaaatccag gtagtatact tgctgtaatg acatcaatga ttgcattggg
     9001 tttttactta caaagataca aagccattaa aagcttagga ccagcactta caataatcat
     9061 aatgggaata atactttcaa acttaaaagt agttccagta agtactgagt tatatggaac
     9121 tatttcaaca tatgctattc caatttctat gactataatg ttgatgagtg ttgacttaaa
     9181 agaaatgaca aaattatcaa gagaaccttt aattgcaatt tttgtagctg tattaactgt
     9241 aagtatcatg gcatttttat ttggattagt atttgcagaa aaaatatcag aaggttggaa
     9301 ggttgctgga atgtttgtag gtacatatac tggtggaagt gctaatctta cagctattgg
     9361 aacaggtctt aatgtaagta gacaaactct tgcagcagca aatgcagcag actatgtaat
     9421 tggagtacca acattgatat ttatgtttgc tctaccagca atattaaaaa attcaaagaa
     9481 gtttaaaaaa ctttggccat atcatgtaga agaatcagaa ttagaagatt gtcaaaatga
     9541 agaatttatg gagtcaaaag aatggagtat taaagatata gcttggatgt tggctatagg
     9601 ttttgttgtt acagaagttt caacaatact tgctggatat ttcaattcaa gttttagtag
     9661 tgcagcaaga atacttttag ttacaacaat ttcaattata attgctcaat taaagccagt
     9721 aaagaaacta aagggaaatt tagatttagg attatttgtt gcattattct ttttatgtac
     9781 aataggtttt tcagtagata taaaagagtt tttaggttca acttttacta taacacttta
     9841 ctgttttagt ataatttttg cttcatttgt tttccattta ggcataacta gacttttaaa
     9901 gataaagtat caatatgtaa tactttctat agtaggagca attgctgatg gtcctacttc
     9961 agctttagta gctgcttctg caaagtggaa ttcgcttgta agtgtagcag tcgtaatggg
    10021 agtaattggt ggagtattag gaaattatgc aggtatatca gtggcatatg caataaagat
    10081 gtctctagga ttataggggg gagtttaatt gataaaattt aaattataca tatctggact
    10141 atacagtgga aatgtaatct ttgatggaga tttattaatt gaaaagttga atccttttac
    10201 aaataaaata gaaagtttaa aaccaataag caaagaagaa aatacatatt atttaaatct
    10261 tacaaaaata gatttaaagt ctttatttaa taattttgat gtatatacta aaagcttagt
    10321 aaacacagat aaagacacag ttataaataa cttaggagaa acgcattcta aacttaatga
    10381 atatatatgg gttcaaagaa acaaaaagtt cccactagat ataattattg tagataataa
    10441 aatagtaggt tttatctgtt tatctagaga aacttgtact atattaatta tggatggata
    10501 tgaagagtat actgtattaa aagaatggga aaaaactcat aaaaatgaag agatatattc
    10561 tataagattt ggtggaaatt atatgattga tatgaaagat ggtataaaac tttctacaga
    10621 tgtatatctc cctgattttg tagattctgc caaaaaagca ccgacaattc ttatgagaac
    10681 tccatatgga aaagaaaatg ataaagaaat atattataaa tatgtacaaa gaggatatgc
    10741 tgttgtaata caagatgtta ggggaagaaa tgaatcagaa ggtaagtggg aacctatgat
    10801 tcatgagagg gaagatggag atagtaccat aaattggata gtatcacagg aatggtcaag
    10861 tggaatagtt ggaatgcttg gagcatcata tcttggttat gtccaatggg cagctgcatc
    10921 ttcaggcaat aagcatctta aagctttagt tagtatagta acatcaggaa gcccttttat
    10981 tgatatacca agaaaaggag gagcttttgt atctggtatg cttgcatggg catttatggt
    11041 atcaagaaat aaagttgata gaagcaaaat ggtaagagat gattgggatg atgttttaaa
    11101 catacgacca atagaaaata tacctgttga agctcttgga tatagaattg aatttctgga
    11161 agaatggtta aagagagtag aaaaagatga gtattgggat ttgatggact ggcatttaca
    11221 aaaagataaa ataaatgtac ctgcattggt tgtatctggg tggtatgatg ataatagtat
    11281 gggaactaca gaggccttag atgttataaa ggactatgaa aaaggaaaga gaaaggctat
    11341 tctaggtcca tggatgcata attcaaatac tttaagagat ataaatggta tttctttagg
    11401 aaatagttca ttaagatatg atttggatta taattatcta ttatggtttg ataaatactt
    11461 aaaaggtata gaaaataaca tagatacgac tgctccagta gagtattact ctgtaggttc
    11521 taataagtgg aagacagaag aaaattggcc aataataaat aagatagata aaagtatgta
    11581 tctaataagt gatggaaatg ctaacacatc tttaggaaat ggtagattag tatttgataa
    11641 tgatttagaa gaaaagtatg atagttatat atataatcca aaagacccat cagtacaact
    11701 aattgatatg tcagagaatg aagttggagt tccaaataac tacaaagatt tagaaaaaag
    11761 aagtgatatg ttgtgttata catcagatgc ttttagtgaa gaatttactg taacaggtga
    11821 tataaagtta gagttttttg catcaagttc tgccaaagat actgactggg ttataaaaat
    11881 tatggacgtg gatttagatg gaaattcaat taaattagct gatggtatac ttagtgctag
    11941 atttagaaat agtttttata agtcagagtt tatggaagaa ggagagattt ataagtttac
    12001 gattataact tcaaaaatat caaacacgtt taaagttggt cataaaatta gactagatat
    12061 aacgtctagt gctaaaaact ttattttcca aaatagcaac acgacagagg gatataatag
    12121 tattgaatat atagaagcaa agaatacgat ttaccatgga ggtaaatatc catctaaatt
    12181 gatattgcca atagagaata agtaaattta tatagattaa atagtgagga ggtgtctcaa
    12241 aatgaatttt ttagttcgtg agacactttc ttttatacga aatcaaatat attttcatca
    12301 tttcaacaat gaaaaagagg cttatctctc tgttttgaga tagcctccta taagctaaat
    12361 tgattatttt gtagctgcaa caacgctcat ttcaactaac aaatgctctc tagcaagtct
    12421 agcttcaaca caagctctag caggttcaaa tcctttttca acccaagcat cccaaacaga
    12481 gttcatagct tcaaagtctt tcatatctct taaataaata gtaacagata gcaaatgttg
    12541 cttatctgaa ccatatttgt ttaataaatc ttctatttta gctaaaactt cagttgtttg
    12601 ttcaattact cctccttcag catgagtttg tccacataaa taaactgtat tgttatgaac
    12661 tacagctcta ctcattcttc cagttccttc atatctcttt atttccatat tttaaatacc
    12721 tccatagtta aaaaatctat taaaaatcta aataataaat taaaaaaagt ctatactata
    12781 taaaattata ttatatattc tgataataat ccataaaaac atataacttt ttagctataa
    12841 ttattattgt tattaattga aaagatttat tagttacttg acatagttta cacttttaat
    12901 tatagtataa attggaatat ttagataatt attatttaat gtataagcta agtttttaat
    12961 taatatggct ttatacattt atatagaaaa gaggtacgta tgagtagcac aatcaaatca
    13021 aatcgaataa aaggtataat gtttataata gcatcagcgc ttggctttgc aatgatgtct
    13081 gcattcgtaa agctagctgg agatttacca agttttcaaa aagtgttctt tagaaatctg
    13141 gtatcagcta taatagcttt ttggcttatc ataaaacata aaggaagtct aactggtaaa
    13201 aaagaaaatt taaaagtact tttatatagg tcaatatttg gaactctagg agttatattt
    13261 aactactatg caattgacaa gttgatgctt tcagatgcta atatgctaaa taaaatgagt
    13321 cccttccttg tagtaatatt ttgtgcaatt gctctaaaag aaaaaataaa tgtaaagcag
    13381 ataggagcta ttataattgc atttatagga gctttattca taataaaacc aacatttagt
    13441 gtagaagtaa ttccatatct tggaggaatt gcaggagctg tatttgcagc aatggcatat
    13501 acttgtttaa gagttcttgg agatagagaa gattactata caattgtatt tttcttttca
    13561 gttttttcat tagttactgt agggccaatt gcttttgcag tatatgaacc aatgaccctt
    13621 atgcaactta tatatctatt actaggtgga gtatttgcaa gtctaggtca atttggaata
    13681 actctagcat ataaatatgc acctgctaag gaaatctcta tatttgacta tagtaatata
    13741 atcttttcag caattttaag tatattctta ttcaatgtct atccagatat gttaagtgtt
    13801 gttggatatt taatagtatt ttcggcagca ttttatatgt ttttatacaa taagaagtta
    13861 gataaattag acaaagaaaa gaagaagtaa actagataaa agtgttttta taatttgtat
    13921 ttaatattta agtctttgta ttaaaattaa agaaaattta attaaaaatg tttggtatat
    13981 taacttataa cagcagttaa aattgaaaaa ttataactgc tgtttctttt attaaaaata
    14041 tccaaatgtc tgtaaaaata gactataata gagtagtata aataatataa aggggataaa
    14101 ttaaagtatg gaaaaagtga aagttgctgt gattggagca ggtaatagag gaacgtatgc
    14161 atatgctcca tatatttatg aaaattctga tgtatgtgaa atagttgctg tagcagaacc
    14221 taaaaaagga agacgagaac tatttacaca aaaatataac ctagatagta aaaatgtatt
    14281 tgaaacttta gaagactttt ttaaacatga caagatggca gatgcagtaa taatagctac
    14341 aaatgatgat agacattatg atgtagcaaa actagctctt gaaaaaggtt atcatgtact
    14401 attagaaaag ccaatgtcta atagtttaga tggtctggta catatagatg acttgtgtga
    14461 taagtataaa gataagattt ttatgatttg ccatgtactg agatattctc cattttataa
    14521 taaattaaaa gaaattgtag aaagcaaaaa attaggagag ctagttagta tacaatataa
    14581 tgaaaatata ggatattggc attttgcaca tagctttact agagggaatt ggagaaatag
    14641 caatgaaacc agccctttaa ttttagcaaa gagttgtcat gacatggata ttttgttata
    14701 tttagtagga agtagatgca aaaaaatatc tgcatttgga agcttaaagc atcttaataa
    14761 ccaaaatgct agtggagaga tggcacaaaa ttgtcttcag tgtttagttg aaaaaaaatg
    14821 tccatattca gcaaagagaa tttatttaga aaaagataga agtataaata gagctgttca
    14881 cataaatcca actgaagaga atctattgaa tatacttaag acaagtccat atggaagatg
    14941 tgtttacaga tgtgataata atgttgtaga caatatggta aatatactac agtttgaaaa
    15001 tggagtgacg gctactttta atttatgtgc atttactaaa gaaaatggaa gaactataaa
    15061 gttaatgttt agccatggtg aagttggtgg ggatttaaat aaaaatgaga taagaattaa
    15121 agaatttgga aaaaacgaag aaatagttat gaatccatca aatcaacaga atatggtaga
    15181 ggaatatgat agaaatctta tagctgaatt tattaaatta gtttcaaata aagaactaga
    15241 aaagggtaga gtagcagcaa aagaagctat acaaagtcat gtaatggcat ttgcagcaga
    15301 atattctagg gtcagtgatg aagtagtgta tatagaagaa ttttttgata gtgctaagca
    15361 aatgactaag gagatagaag aaactatatt ttaattaata aaaatttatt aaataaaata
    15421 aagatagatt aaataataga aatacgaaaa tatgtgttct attaaggaat ctatcttttt
    15481 tattaaggtt ttttcttatc cctatcaaaa aaatccatac attctgataa aagataagaa
    15541 agtaaagcga ataagatttc tgtacttaaa ggaatcgtta ctgaatccat aaaggtatca
    15601 attatgttca tagctagaaa agttagtata aaatcaatta taaattcgca aagtttatat
    15661 attgagtatg gtaatttgtt tacatatctg aatatatcta aaatagtagt gcatagaaaa
    15721 tcaattggag tggttataca aaaatatatt aaaaagaaaa ttaatacagc tccaagagat
    15781 ttatattcaa atcctaagaa tgataagaag ccaattccta taaccgtgaa aatacaaaaa
    15841 gttataccaa aaaaaacagc aaaaaataaa aatacagaaa taaattcgcc taaaggaatc
    15901 cttcttttat tacttatctt aatcaactcc ttaatttatg atatgaaatt tatataaata
    15961 ataacattct taaaaattca taatagtcct ctgtttcaaa tagtactgtt tgagaatcta
    16021 tttttttcat atttggataa aaagaggcct taaaagcttt ataatgttct ctaaatttta
    16081 tttctatctt aaatttatct ggcaatttta ttaagtgcct agataaatct ccagataaag
    16141 cttttttgac acctttttca atttttttta gtgcaaggtt tggatggatt gaaattgaac
    16201 catttccaat tccttttgac acaggaacag tgactatatt tggatttagt ttttttgcac
    16261 tctcacaaag catttcatct ccacttaaaa atgctacagg aacattataa tatgcagcaa
    16321 tataagcatt tatagtaaat tcagatgcta tttcatcatt tattttaaaa tagtcataat
    16381 caatatccat agtatgagaa agtggattac cattacaagt agctccagag tgatagccgg
    16441 tgaatataca tgcatcaaag ctttcatcaa ttcctgccat cataactagt gggtctttgg
    16501 tccatcctct aagtattttg acgttttcag gtaaaatatt tggattaata tttctaccac
    16561 tgtcatgtgc atctttaaca agtatttcat gtacaccaga ttgagtagct ccatcacatg
    16621 ctgctgttat ctctttactc atttgcgttc tatagtgttg cgaaaattca cattcaagtt
    16681 ttgtttcatc ccaatttaca ataccacaag taccttctat atcagcactt aaatacaatt
    16741 tcataatata taccccctta tgtttattat tgatgataat aacagataac agaaaataca
    16801 taatcaataa agggcataaa aatattatgt cttactatta actattatta aatctgtaac
    16861 tgaaaaataa ctaaattggt ataaaaaaat ataacactat gtgtcattta tttatatagt
    16921 tttttatatt tgatgtttat ataaaattgt aacatataag tggttacatt tactagttta
    16981 acatagttaa aatagttagg tattattttt aagttaaatt gattattata aaatcgcaga
    17041 ataaaagaaa aatttaattt attaaaatgc atgaaatgtt ttttcctcaa cataatgcta
    17101 aattaaactc aaaaagaaat aatagaaaaa tcttatgtag cttagaaaga atgtgaatga
    17161 tttaagggaa aaatagaaat ttattatata atattaggtt gtattatata atacagccta
    17221 atattatatg tatatgtgac ataaaatctt ggtaagtgta aataaaataa atgtggaaaa
    17281 caagttaatg aaatcaagaa aaaagtaatt taaaattcaa tttcatcttc ccaattaaat
    17341 tctcctgatt tttctacact cttaggccag tgactacacc atttaggaac ggacggattt
    17401 tcaaccctat caacgcttgg agtatatggc tttaagtcaa taacaggact attatcatta
    17461 gcatcaatat atggtatttt tattatgcca ttttcatggt cgatattgat aatttgtaca
    17521 gcagtcaatg ctattggatt gggtcttata ggtgaccttg ttgcaaatat tcctataacg
    17581 tctggtccac ttttataagg ttttggggtt tctaaaatac ttcttagttc aggattatca
    17641 aacccatcag cccaccaaaa tacatttaaa tgactgaaat cgtcgagttc atttaatgca
    17701 ggtacatact ttttgtctaa tttaataaag acctcttcgc cttgaataca tattttacct
    17761 ataggattta ttaatatttc tttcataata ttacctccaa ctatttaatt tatcatttgt
    17821 aattttaggg tataacatga catcatgtga gggtcaatat aaaataataa ataaaaatat
    17881 taatagttag aatgataata cattttttac atggagcatg acaccagtaa taaggcaatc
    17941 ttcgtcaata tcaaaagtag aggtatgcaa aggggaagta atgttttttt cacgatttgc
    18001 acaaccaagg tggtaaaaag ctccttcaca gtgtcttaga aaatacgaaa agtcttcagc
    18061 accaagagtg ggcgagtctt taaggataat attttcttca ccaaacaatt ctacagcatt
    18121 ttgtttgacg taatctacta gatggtttga attgataaga gctggataac cttcctcaat
    18181 ttcaacagaa atacttcctc caaatgtttt acaagtaaaa tcgcaaattt ctctaattcg
    18241 tttttttgaa aactctctag tttcaggagt caaggttctc aaagtacccc ttataaccac
    18301 tttgtcacaa actatatttt ctttgattcc accttcaatc tttcctagag aaataaccac
    18361 agaatcagtt ggatttgtgt ttcgactaac gattgtctgt agagctgtaa taatatgagc
    18421 agatataaca atagcatcaa taccttgatg tggataagct ccatgacatt ttgaaccatg
    18481 tacagttaat tgaagtgtat tggtggaagc atttagagtg ttgtatttta attcaattag
    18541 atttttgttg atattgggat ttacatggag tccaaatata tattttacat ttggtttttc
    18601 aagacatcca tcttctatca taagttgagc cccaccaata gtttcctcag caggttgaaa
    18661 aaagaatttt acatttacat ttaagtaatc ctttaattta aatagtatat tacaagtacc
    18721 cagtaaaata gcagtgtgtg catcatgacc acatgcatgc attttgccaa tattaataga
    18781 tttataagaa taattaagtt cttctgttat aggtaaggca tctatatcag ctcttatagc
    18841 aactgtttca aagtttggac tcacgttaat gtatgcagtt atccctgtat gattcttata
    18901 ttcaatataa tttataccta tttcatttaa gtatttaata atttttttct tagtttgaaa
    18961 ttcttctaat ccaagttctg gagtcttatg taaatctctt cttatattta taacccaatc
    19021 atttatctca tgacaaagag tttttatatt aagcataaaa cacatcctaa ctatattaaa
    19081 aatttatttt tcttcattaa ttatattatt ttatgtaaat cattattatg tatcaagtac
    19141 aaataaaata tcttataatt atattagaga tatttagtat ggagaagaat atatcatttt
    19201 ataagaagag gtgtttaaat gttaggaata attgcgccat ctgggccatt aagaaataca
    19261 agtttggagg aaataaagtt taatttagaa agttatggtt acgaagttaa gttttctgaa
    19321 agttgtagtt taaattataa aggttattta gctggtaatg atgatattag agcaagggat
    19381 atagaagata tgtttttaga taaagatgta gatataatta tgtgcttaag aggaggttat
    19441 ggaacaacaa gaatattgga taaaataaat tatgatataa taaagcaaaa tccaaaacca
    19501 tttataggtt tttctgatat aacaggttta aatttagctt tctataaaaa ttgtggtctt
    19561 ttaccatatc atggaataat ggcagcaaat gtaggtaaat ggggtaaatt tacctataaa
    19621 tcattggtaa atgctttaga atttaaagat gaattatatt tagaaaatcc aaaagaagaa
    19681 aagatttata ctgtatgtga aggtaaagca gagggaataa taatgggagg aaatttatcc
    19741 ttaataattg ccacaatggg aacaaaatat gaaatagatg caaaagataa aattttgttt
    19801 attgaggaaa ttggagaacc tcaatataaa ctggatagaa tgttaacaca actatattct
    19861 tcaggaaaac ttgaggaatg taatggtata atttttggag attttaagga ttgtattgaa
    19921 gaaaatgatt tgatggaatt actaatagaa tttgcaaata aggtcaataa accttctata
    19981 tataatcttc agtcaggaca ttgtatccca atgataacta ttcctttggg cagaatgtgc
    20041 gaattagatg ctacagaaaa aatcgttaaa ttaaaaaaat agtgtgtata taaatataat
    20101 agaaatatgg tataatctta tacaagtaaa aatttagcta ggaggatgaa aaatgtctga
    20161 tgatagaata atagacttta acgaactaaa gaacaaagtt aaagattctg atgtagataa
    20221 atttgagcaa tacatatata acttatattt ttctgttatg gatggaaaga tgtctatggc
    20281 tgagttttca agaaaaatat tcgattatat gagagataac aacatatctc aagaaaaatt
    20341 tatgaaaata caaaagcaat ttatggagag atatggtatg gacactgaag aagtagaaaa
    20401 acaacttaga aactttggaa tcgacccttc tacagcaggt tttatgagta ataatacttc
    20461 cagcaaagta tctacagaag atttagagtc atttaagaag agtgcaggat tttatgagaa
    20521 atatggagaa aaaatccaac caaagagttg tataacaaca tttataaaaa atgacttaaa
    20581 tgatattaat gtaataatcg accaagaaaa gataatgctt tgtagtgata gaaaaataaa
    20641 tcttatggat tcagaactaa atgagttttt attagaatat aagaatatgt ttaataaaaa
    20701 aataaaggta gtaatgtgcg aaactacgaa taagtatgat tattaaaaaa tgaatttaaa
    20761 tactgaagta gtataaatta attgtactac ttaaaaataa aaatcgcaat ttattattcc
    20821 ttataaattt gtataaggaa tatctaaact gcgatttttt atattgtgaa gattagtaat
    20881 aatagatagt gttttaaatt gtaatcttct cactaaaact gtgtttttct attagaaatt
    20941 ttattaaaaa taaatatctg ataattgtat tatatgggat gtaaagtatt agaagcttaa
    21001 cgaatctctt ttaatacatt aactccattt tgaatcatac tgttgaaaaa tactaagttt
    21061 aaaatctcag cagggtgaac gttaggaata tcataagtat ctactaaatt cataggtaca
    21121 actatatttt tttcaatatt atgttgatta aaatatgatt ttagagttat tgcaaattga
    21181 tatatacata tatctgtgca atcgccaact ataattatat tatctatatt atcaaaatcc
    21241 aaattttcta atgcaaaaaa tccattagta gaatttttag gaagaatttg taggttttct
    21301 atcgacttaa gttcatcaac caattcactt tctacatcat tttccaaaca gtgaacagga
    21361 taactcaaaa gttctattga tttaggagta tgagaatcag taaatgctat tactctattt
    21421 aatttgtttg atattttttt tgtaaacatt tcaattggct ttattaaaga ctctactcta
    21481 ggagagtata atgcaccttg tcttgcaaag ccattattaa catcaattat aaataaagca
    21541 gttttactca aatcataatt ttctattttt tctattggta gattatctaa attactcttt
    21601 aaagcttcaa gttcatttaa taagttatcc atagttatac ctccaagata aatttaataa
    21661 aattgtacat attaattata taaaagtcaa ggagtacaaa ctattgtata attagctgta
    21721 ttgttttgag tgtaaaagat ttgaagcaat atctggatag ttagtaaaaa taccatctac
    21781 attgagtttt agacagtgta gtatatcatc ctcttcattt acagtatata cattgacacc
    21841 aagtagattt ttgtgacaaa gttcaattag agattcattt actaaaacta gaggtggatg
    21901 aagataacta ttacccagaa atttagcaaa attgactaca ttgtttatag gtaaataaca
    21961 caataatcct gtttttacac tagggtctaa tttatgaaag ttttttaagc tattgctgtt
    22021 gaaactagat ataagaactc tatttttcat attatacttt aaaataagct ctaaaacgcc
    22081 tttttctata ttgggatagt ttattttgtc agtttttagt tcgatattta aagtaaggtc
    22141 tgtaggtgag attagaatta atacttcttc taaagttgga attttacatt ctttattgct
    22201 ttgatatcct tcaaaagatg agtttagagt ttttaatttt cttaaagtaa agtcttttac
    22261 aaaaccatgt ccattaaaag ttctatcaac cttttcatca tgaataacta caatttttcc
    22321 atccttagtt ctgtggacat caagttctat tccatatata tccatatcca aacatttttt
    22381 aaaagctaga atagtatttt ctggatactt tccactaaac cccctatgag cataaatatt
    22441 catttatatg gttccctcct ataagtataa cttatcttat aaaaatttaa ttaatataat
//
LOCUS       DAEOSP010000046        22476 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.046, whole genome shotgun sequence.
ACCESSION   DAEOSP010000046 DAEOSP010000000
VERSION     DAEOSP010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22476
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.046"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            134..2677
                     /locus_tag="KRH54_001438"
     CDS             134..2677
                     /locus_tag="KRH54_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBG8579105.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESVSQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGVKEEIDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERNVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            2800..4791
                     /locus_tag="KRH54_001439"
     CDS             2800..4791
                     /locus_tag="KRH54_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579106.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNKDTGVKYIYSFSDNKEKLEAVLEDSQIIIYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            4781..5101
                     /locus_tag="KRH54_001440"
     CDS             4781..5101
                     /locus_tag="KRH54_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579107.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            5299..6168
                     /gene="galU"
                     /locus_tag="KRH54_001441"
     CDS             5299..6168
                     /gene="galU"
                     /locus_tag="KRH54_001441"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBG8579108.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            6192..7205
                     /gene="galE"
                     /locus_tag="KRH54_001442"
     CDS             6192..7205
                     /gene="galE"
                     /locus_tag="KRH54_001442"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBG8579109.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            7317..9251
                     /locus_tag="KRH54_001443"
     CDS             7317..9251
                     /locus_tag="KRH54_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBG8579110.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            9406..10269
                     /locus_tag="KRH54_001444"
     CDS             9406..10269
                     /locus_tag="KRH54_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBG8579111.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(10477..11358)
                     /locus_tag="KRH54_001445"
     CDS             complement(10477..11358)
                     /locus_tag="KRH54_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG8579112.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            11771..12619
                     /locus_tag="KRH54_001446"
     CDS             11771..12619
                     /locus_tag="KRH54_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBG8579113.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            12698..14620
                     /locus_tag="KRH54_001447"
     CDS             12698..14620
                     /locus_tag="KRH54_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG8579114.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            14702..15565
                     /gene="aroE"
                     /locus_tag="KRH54_001448"
     CDS             14702..15565
                     /gene="aroE"
                     /locus_tag="KRH54_001448"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG8579115.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            15765..16982
                     /locus_tag="KRH54_001449"
     CDS             15765..16982
                     /locus_tag="KRH54_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8579116.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            17218..19083
                     /locus_tag="KRH54_001450"
     CDS             17218..19083
                     /locus_tag="KRH54_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBG8579117.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(19261..20625)
                     /locus_tag="KRH54_001451"
     CDS             complement(19261..20625)
                     /locus_tag="KRH54_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8579118.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(20804..22147)
                     /locus_tag="KRH54_001452"
     CDS             complement(20804..22147)
                     /locus_tag="KRH54_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG8579119.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(22220..22321)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         8968 a         2513 c         3908 g         7087 t
ORIGIN      
        1 aaaagtaaaa tgatgtaaat aatatgatat gatataagat gtagcaaatt tagtaaataa
       61 ttatttagat atcataatca aattagcaac tttgcacaat gtaaatatta caataaaata
      121 attgatttaa ggagtgaagg ttttgagtga aaataatata ataagaaaca ctgttttaaa
      181 tgtcacagca agttttttaa aacaagagtc taaaataaat gaaaagctag atggtgtttt
      241 ggaaaagaaa tttgaaaaag tagaatttaa tgaagcaaaa tatgcagagc ttttaaaatt
      301 taatatattg ttttataaga cattagcaag aaatacagaa ccacttattg gcaaatggat
      361 agtagataag tacatacctg aaatagatga attagaaaaa gatttagaat taactacagc
      421 aaaatgtaga aagtatgtaa ataaggctat gaaagaggga ttagactctc taaaagccaa
      481 tgatttaaat agttttttag cttatgataa aatggaacta tcagagagaa gaagacgttt
      541 agaaaaggat tataaggttt taaacctata taaagatctt cttaatatat ctcttagaaa
      601 aataagttta gaaaagaaag aatgtggaga tttatttctt aaaaatcaag cagatgcgaa
      661 aagagaatta aaaagagaaa ttatattctg tgtaaacaaa atattagcta gcaataaaga
      721 tgtggtacct aataatgtgg aagagaataa cgaacaagac aaagtagaaa taaaagcaac
      781 tgaaattgag gatttgcaat taggtaaaga taattcagaa aatgacatgc tattaaatga
      841 aattaaaaat tcaaaagtaa aacatgaatc aaaatcaaat gatatagaat cgaagtttat
      901 agataagtta aatagcttaa ataatgaaag tgtatctcaa acaatacaca atgaatataa
      961 gaagttatgt ggattagaag aacttcattc tgttgaagga tatggttttg gcaaagaaat
     1021 tattaaagat tttgcttgtg caacggttgt attagaattt ttaaagagaa gaaatagaga
     1081 ccttatagaa ggtgcaatga gactgactat aattggagaa tttggtcctg aaaattttaa
     1141 ggaatttgtg gactatgtaa ttaaaaataa aacagaaatt agtgaagata catggaataa
     1201 agcacagaat ctaataaaag ataactatag tgaattagaa aatcatgaaa ttgcatcaaa
     1261 aagaactaga agaaataaag atatcgatat tgaagaatac atttacatga taaaaaatgc
     1321 agataaagat atttgcttta gaagtagtat ttcaatagaa gatgatgctg aagaaggggt
     1381 taaagaagaa atagatagta ataatcaaga tataggtgac gttgtagaag ataaagatac
     1441 tacagataaa gagtacgaca gcaataaaga agatataatt gaaccagaaa ataaaaagag
     1501 taaaaagaag gctaaattat ttggatttat taaaaaagac aatgaagaag ttgaacaaga
     1561 agaagagaat ttaaatgata tttctcctga tataatttta gataaaccag ttgaaaatca
     1621 tcaagtaaaa tcagaagaaa tagagcaaaa tgaattaaaa gaaataaaac aagaagaacc
     1681 aagtcaacat atagaagaag aacgtaatgt aaaaatagaa aaaccaataa ataataactt
     1741 agatgaaaaa gtatcttcaa ataatgaatc gaagcttgaa aaagaaagta aaaacttaga
     1801 agataaaaaa gcaaaagaaa taaaagaaga aaaattagaa aatgaaaaat cagtagtaat
     1861 acctattaaa aagaaagaaa atagtaataa aaaatctaaa aattcttcta aggacaaata
     1921 tagagaaaac aagaaagaaa tgaaaaatta tgtatcagat aaagaagatt ctttagatga
     1981 tgaagaggtt gtttctaaga agagtagatt aaaagaaact attatagcag tagttatagt
     2041 ggttattgtt ggtgtaggat attttataac agtaggcaac aataagaaaa atgataaaga
     2101 gaatattcca aagtcatcaa ctcaacaaca agctaacaat aaattaactg aagaagagaa
     2161 aaaagcacaa gctgaaaaag agaaaaaaga agcagaagaa aaggcaaaag cagaaaaaga
     2221 ggctcaagaa aaagcacaag ctgaaaagaa agcaaaagaa atggaagcat ataaagatgg
     2281 taaaggtgta tattacactg tttatgcagg ttcacttaaa gttgaaaaaa cagcaaaaga
     2341 aacagcaaaa gaatatgagg ctaaaggtat ttcttcaaca ataattcaag aaaatggata
     2401 ctataaaata aaaataggtg attattctca atatggagaa gcacaagaaa aatgtaatga
     2461 actagctaag aaatctattg atacatatat agctatgtat gataagtact atgattataa
     2521 gttagaagaa ttaaaagaat cagctccttc tttgagtgct gaagaactta aacaaaaata
     2581 tgaagattta agaagtgagt taaagaacaa atctggatat cgtgaatatg ttaaacattt
     2641 agataaactt tatgaagaaa tagttgaagg tgcttagagc attatagaaa aggagaaact
     2701 atgtctcaga ataaaaagta ttaaaaaaaa tattatttat tgtgagacat tttgttcacc
     2761 tttataaaat acttataatt ctgtttaggt ggtgaaggta tgttgaaatt aggagaaaaa
     2821 atcatatatg agctttcaga tgggtttgag cttagtggca taagtagata ctcaaaagaa
     2881 tataaaaagc ttcatataac aaatctaggg aatataataa tcagcaaatc agatgaagtt
     2941 aataaggata caggtgtaaa atatatatat agtttttcag acaataaaga aaaattagag
     3001 gcagttttag aagattctca aataattatc tatggaaata acttgccttt ttacataaaa
     3061 agtgaaaatt caagtaaaaa tgttacagca aatttgacta tgaaaataac tttggatgaa
     3121 tatgatttaa tctgcaaaag tcaaagagaa tttatattct atctaaagga taatatgact
     3181 atattaagtt tagatgacaa taagttttat atgggtggaa ttaataaaga taatgagaag
     3241 tttatattta tcagtgggaa aaatagattt gaaataaatt ttgatgatat agaaagatac
     3301 attttagaag ataaaagagt gtcattgaag ggatactttc atatggaaag agaggggatt
     3361 atagtaaggt ctgtatctat atttaataac aatatagata gtgtagtccc atctgattta
     3421 aatgagagag ttaaggataa ccaaaaaata ggaaatcttc ctaaagattg tgaaatagta
     3481 ttctgcaaga tatcaggaaa tatagatgga tttgattata aaaacacaaa catgctttta
     3541 gtaaaatatc aagaccaatt aatttttata aataagaaat ctaagaagac tattgtaaag
     3601 tcagcgaaag ataattgttc taagttaaat ttaggagaag atataatatt atatgataat
     3661 aaaaatgtat ttaacctaca tataaatgat aaaaatagag aaattatgca aatagatgac
     3721 ctaaaagata tagaaaatga aatagtggga tatacattaa aacatgctcc attttttatt
     3781 caagaagatt tcgatagctt gactattcta aagtcttttc aaaaagaaat catatctata
     3841 aaaaatagtg atataaaaga cattgtaatt aataaagaac ttgaaaacga aaattctaat
     3901 tttgtagaaa cggaaataaa atttaataat caaaaagtac ttttaaactt aagtaaaagt
     3961 atggttcaaa aacttatgca agatgtattt atatatgcaa agcaacctct acttaaagaa
     4021 aattctattg aagtgatata taagaattgg tctaaagcta tgaatgatat gataatattt
     4081 aatttctttg gaaatattta ctatatgaaa tctgaatttg ataagatttt agaaaaagag
     4141 ttaaatgatg aaataagaat agaagttata aattcacttt ataagcaaat acaagaacaa
     4201 agaaacaatt tagatttatt gtcagcatat atgccaagaa tattagaaaa tcaagaaata
     4261 gatttgtttg aaaagtataa tactaaactt gatgtacaag tgtttaagca gattaaaaat
     4321 ctattgtctg atttatcata taatataagc agttatttaa atgaagttga aaaaagttta
     4381 gacaatatca tctttgtaat ttcaggagaa gataagaaga aatacaatta tagaatgtta
     4441 aaagaaagtg aaagtgcaag tcttgatgta tttttaaaac aagccatatc aagattaaac
     4501 catttagtag aaaatatgta tccttattat gtagatgaaa ctagtaggga aatgtttaag
     4561 ttatttgagt tattgtggaa aaactataga aatattgatg atgatagtat aaaagaaata
     4621 ttattcgaga gaataacaaa tacatatgta tttaaacaat tgacattaaa taatagtaca
     4681 aaagaaagaa gaaaagacat aattgaaaaa atatataact ctgttgatta tggaaccaat
     4741 aaactagatg aaaatatgtt tttcacagga ggaataaagt atgtcaagta aatacaatgt
     4801 ttggactttt aactatgagt tcttaggatt ggactcaggg gatgaaaaaa ataatgaagt
     4861 ttattacgag attgatttag atgaaatttt aaatgaaaac tatatagatg aagatataga
     4921 tagtggttta gattctgatg gaaatagtat tttaggagaa ttaatatcag aaatggatat
     4981 agatgaggat gtcagtgttg atatgacata tcaagaacta gaagttgact tagaagattt
     5041 agattcttat ctagatgaag atatagatag tgatataaaa ggcatcttag atgaaattta
     5101 gtacctgtat aagttttgga taaaatataa acaatacaat attaacttat aaaactaaag
     5161 gtagatgaaa tttcatctac cttatattat cttatagcat taaatattgg gataatatag
     5221 agtttgaaga aaaagtataa atatggtata ttaatttggt acaatagaaa atttacgaag
     5281 aagggtgaaa aaatgcagat gacagttaaa aaagcaataa taccagcagc agggctagga
     5341 actagatttt taccagcaac aaaatcacag cccaaagaaa tgcttcctat agtagataaa
     5401 ccaactttac agtatataat agaagaagct atagaatcgg gaattgaaga aattcttata
     5461 ataacaggta gaaataaaaa atctatagaa gatcattttg ataaatcagt tgaattagag
     5521 ttagaactag agcaaaaagg taagacagag atgttagaaa tggtaagaga catatcaaat
     5581 atggtaaata ttcattatat aagacaaaaa gagccaaaag gtcttggaca tgcaatttac
     5641 tgtgcaaaaa gttttatagg tgatgaacct tttgccgtat tattaggtga tgatatagta
     5701 gattcagatg ttccgtgctt aaaacaatta atagatactt ataatgagta taaaacaact
     5761 gtattaggtg ttcaaaagat agctaaagaa gatactaata aatatggtat attggatgta
     5821 aaacatatcg aagatagagt atataaagtt aaagatatgg ttgaaaaacc tgcaattgaa
     5881 gaagctccat ctgatatagc tatacttgga agatatataa taactcctgc aatatttagc
     5941 atattagaga aacagacacc tggtaaaggt ggagagatac aactgacaga tgcacttcaa
     6001 actcttggaa aacaagaggc tatttatgct tataactttg aaggtagaag atatgatgtg
     6061 ggagataaga ttggtttttt agaggcaaca atagactttg ctctaaagag agaaaattta
     6121 aaagatgatt taatggacta tatgagaaaa aaagtaagtg aaaaataaat attagaaaag
     6181 aggtaattac aatggcagtt ttagtagctg gaggagcagg atatatagga agccatacag
     6241 caatagaact tttagaatca ggttatgaag ttgttatagt tgataactta agtaatagca
     6301 actcaatagt ggttgataga atcaaggaat tatctaaaaa accagtaaaa ttttataata
     6361 ttgatatcag aaataaagat gaaatgcatg ttgtatttaa agaaaataat attgaatcaa
     6421 ttatacactt tgcagcttta aaagcagttg gtgaatcagt agaaaaacca attgaatatt
     6481 acagcaataa tctaataagt accttaaatt tatttgaatt aatgagagaa tatggtgtta
     6541 aaaagtttgt atttagttct tcagctactg tatatggaga cccacatact tgtcctatat
     6601 tagaggattt tccactttct gtaaccaatc catatggaag aactaaactt atgatagagc
     6661 agatgttagt agatatatca aaagcagata agtcattgga tatagctctt ttaagatact
     6721 ttaatccagt cggtgctcat aaaagtggta gaataggtga agaaccaaat ggagttccga
     6781 gcaatctaat gccttatata acaaaaatag cagtaggtaa attgaaggaa ttgagtgttt
     6841 atggtaatga ctatccaaca catgatggaa ctggagttag agactatata catgtattag
     6901 atttagctgc tggtcatgta aaagcattac aaaaattaga agaaaatcca ggacttgttg
     6961 tttataattt aggaactgga aaggggtata gtgttcttga tttagtaaaa gcattttcta
     7021 aagcaagtgg aaaagaaata ccatataaga ttgtaggaag aagggctgga gatgtagcca
     7081 tgtgttatgc agattcttca aaggcagaaa aagagcttgg atggaaagct aagtatgaac
     7141 ttgaagagat gtgtgaagat tcatggagat ggcaaagtat gaatcctaat gggtatgaag
     7201 aatagaatat atacttatat gtataaatgt aacaaatttg taaatataag gtattggtaa
     7261 ttttatgtta taattagaca catgtagtaa tgtttctaaa agagggggaa gttatagtga
     7321 aaaaaagtct aaaaaagtat ttagtactag cactgacttt agttttagta tttgcatgtg
     7381 gcgtaccaga atcaagcgca gccagtaaac atgtgattat agtaaattca agaaaaaata
     7441 ctctaggata ttttgtaaat aataaacttg taaaagaatt tagagttgca acaggtaaaa
     7501 aggggtcaga aacaccaaca gggaaaacaa aagtagttaa caagataaaa aatagaccat
     7561 attataaagg taatatacct ggaggaagtc caagaaatcc attaggagat agatggatgg
     7621 gattagcatt aaaaggaact tatggagata catatggaat acatggaaat aataatgaga
     7681 gttctatagg aaagcatatt tctggtggat gtataagaat gcacaataaa gatgttagat
     7741 ggttatttga ccaagttcct gtaggttcag atgtaattat agattatagt aatgacagct
     7801 atgtaaagat agcggcaaaa tataagataa atctaaatca aacaggatgg aaaacagaaa
     7861 atggtaagaa gtattatgta aaatctgatg gaacatacca aaagaatagt tggctaaaag
     7921 taaatggaaa aatgtattac tttgatgctt caggagtaat gcaaacagga tggaaaacaa
     7981 ttaataacaa aaaatattat ttaggaactg atggagcaag agtatctgga tggaaagtaa
     8041 tagacggaaa aacttattac tttaactcag atggagtaat gcaaacagga tggcaagaaa
     8101 agaatggaaa gaaatactat ctaggatctg atggtttagc agtaacagga tggcaagaaa
     8161 tagatggtaa taagtattac tttgacaaaa caggaataat gcagacagga tggcaacaaa
     8221 tagatggtaa gtcttactat ttagacaaag atggaaagat gctaactggt tctcaaaaaa
     8281 tagatgggaa agattatact tttaatgaag atggaactat aaatcctaca tgggatacaa
     8341 ttataggtgc aaatagattt gatacagcta aaaagataag ttcagttgga aactggaatg
     8401 ctgatagctc agatactgta atattagtaa atggtaatgc aatagctgat ggtataacag
     8461 caactccttt agcaagtagc tatgactcaa caatactact tactaataca gctaatttac
     8521 caactgaaac tgttgaagaa atgaagttat tagcacctaa aacagtaatc ttgataggtg
     8581 gagaaaatgc aataagttct aaattagagc aagaaataaa aactactttc aacacagaaa
     8641 caaagagaat agcaggtcaa gatagatatc aaactgctac aagaatagct gaagaacttg
     8701 gaaatcgaga agaaataaaa actgcatata tggtttctgg aaatggggag gcagatgctt
     8761 tatctgtagc ttctaaagct ggggaagaaa aacaacctat aatattagtc aataaagatg
     8821 gaattacaga agaatcttat aaatggctaa cagaaagaaa attagaaaat gcatacttta
     8881 taggtggacc aagtgcaatt aacgattcag taatagcaaa aatgaatgat ataactactg
     8941 aagatatttc aggaaataga atatatggag atagtagagt agatactaat gccaaagtaa
     9001 tagaaaaatt ctatggagat actgatttac aggctgtatt agttagtaaa tcagatgctt
     9061 tagtagatgc tttatcagct ggaccactag ctgtaaaatt acattcacca atagttttaa
     9121 tggataattc aggattatca tcagagcaac aaagagtatt tgcaaataaa aaagttgaaa
     9181 ctccttatca aataggaggc ggagtatctt atatagtaat ggataaatta atggatattc
     9241 ttgcaaaata gtaaaaatat atattgaaaa ataattataa ttgagatgtg aaattcaatc
     9301 aataaaagct tgattgtttt acatctcaat tttttagtat aaaattaagt ttaagttata
     9361 taaaaatctt ggaatatttt taaaactcat ataaaatgaa tatagatgct atcatcatat
     9421 gttaagaggg tgagtaagat tataaagaaa aaatatataa tgtttattga tgaaagtgga
     9481 agagctgata taaatcatga agacccattt acattgacag gagtaatttt tgaatataaa
     9541 tatgcagtaa gtcaaggcga ttatgtatgt tctttgagga aagaaatgga tgcatttaaa
     9601 gagtattgtt ttggaactag taacatagga atacatttga cagatatatc aagaaaatca
     9661 agagcatttg agaagtttga tgattcacag attaagttat tctatgatga gttgccttta
     9721 tttctatcta gactagagtg tactataata tcagtaacag tagataaagc tagattaaag
     9781 aaatattatg caccttcaaa ggaaccgtat gttgtggctt ttatacatgt tcttcagaat
     9841 ttttattcat ttgtaaataa tgaaactgta gaatctgcac gaatagttat agagtcaagg
     9901 gatgatgtct caaacttaaa agtacaaaag gcgttttttg atgtatttaa taatggaaca
     9961 atatacttag atatagatga agaattaaga gataaagtta agggttttat aattgccaaa
    10021 aaagaagatg ctgactatag agaaggttta gaaatagcag atattttatg taatcctctt
    10081 agcagagcaa gaagagggtt aatagaagct aatccaaagt gtatgcaata tggagaaaaa
    10141 aacaaaatat tcaaatctgt cagaaacaaa atatatactc caacgagtgt gaatgatata
    10201 agaaattggg gatttaaaga agttcctatt gtagagcctg ttggaccatg gcttgaaaaa
    10261 atgcgttaga tacaattgac aaattttaaa aaatctgata taatgtaagt aacctagtta
    10321 ttcaggaact gaatccagtt gctgttgtat aacgataatt actgagactc ggatgccgac
    10381 tgtcagttag gaatctatac ttttaaaagt atagattttt ttattttata aaaagaataa
    10441 aagaggatat ttaaaaataa cccctttatt aataaattat aattgagcgt ttttaatgac
    10501 aaagttttta aagtttttaa ttgcaggagg caaatatcta tcttttataa atgctaagta
    10561 aaagtttctt tcccaacttg ggtgaataat ctgaattact tttacttttg taaagctaag
    10621 catattcata tttgggacta cagcaattcc gaaatttttt gcaactaatc cagcaattac
    10681 ttggtcttcc tctacttcat agactatatt aggctgttga tttatttttt taaacatatc
    10741 atcaattata tatctaagac cgcttctctg attaaatact atttgaggat aaggtatggt
    10801 ttcttttaaa tctatagtat cctttgcagc tagtggatga ctatatggta caattagtac
    10861 taaatcttgt ttggcaacag gtataaattc aattcccttt tctttttcaa gtttcgaaca
    10921 aaatgcaata tcatattttc tttctttaag accttgaata atgtcagtag ttacaccagt
    10981 atgaaattta aaatctattg atttagcagg attagatttt aaaaatttgt gcactatgtc
    11041 tgggataaaa tcagtaccaa gtgttctaag aaaagctaag tctatttgtc cttcaccagt
    11101 accagttatt tttaatgatt ttacacttga atctaaaatt tctaatgatt tttccacatc
    11161 ttttaaaaat atttttccat acttagtaag gactatattt ctaccttctt tttcaaatag
    11221 agccacccca agttcatttt ctagtaaaga aatagcatga cttaagcttg gttgtgttat
    11281 agataaattt tctgccgctt ttgtatagtg ttctaaatgt gccaatgtaa caaaatagcg
    11341 taagtgatat aaattcaatt tttgttcatc ctttcatagg tatttttatt tatatatttg
    11401 tagaagttaa cctttaagat atttttacat ttttattata tatgattata gagtaagaat
    11461 aaaagctttt aaaaaaattt atcgtttttt gtctgaatta taagataaat atatttatac
    11521 gacctaatag aaaaatacaa gaattatctt ataaataaaa tctataaaca aaatctatga
    11581 attaatagga aattatcaat ttgttattag taaagtctcg tgatacaata aatttataaa
    11641 atctagataa atagaaatca aaaaatactt ttatttttaa ggtaaaataa tactactagt
    11701 aggataaata aaaatataaa atcaaaaata ctattaatta taaagaaaat aaagggggca
    11761 aatttaagct atgaataatc aaaaaaatct taatattcca attacaatta gttcttatac
    11821 attgggaaca gaagtttcat tccgtgatag agttcgtatt gcaaaagaag ctgggtttga
    11881 tggtattgga ttgcgtgctg aaaattatat tgatgcaaaa aatgcaggtg ttacagatga
    11941 agaaatgatt gcaattctta atgaatatga tatgaaagtt acagaagtag aatatataac
    12001 tcaatgggga agagaagaag atagaacgga agctcaaaga gaaaaagagc aaaatgttta
    12061 tcatatggct cacttattta atgttaaaca tataaattgt ggattattag agatgatacc
    12121 agaagaacaa attataactg cattgggaga attatgtgac agagcaggtg aacttattat
    12181 aggtcttgag tttatgccat atagtggtgt accagatttg gctactgcat ggcgtgttgt
    12241 gaaaggatgt aatcgtgaaa atgcaatgct tatattagat acttggcact gggctagagc
    12301 aaagcagaca gcagaggacc ttgcacctgt tccagcagat aagatagtat caattcaagt
    12361 gtgtgatgtt ttagaaactc cttatgaaaa acttagggat gaatcactac atgaccgttt
    12421 ggctccaggt gaaggatatg gaaacacaga ggaatttgtg aaaataataa aagaacacaa
    12481 tatatcacca agagtcatag gggcagaagt aatatctgat tctctaatat ctaaagggtt
    12541 atccactgct gctgaaactg tatttaatgc aactaaaaaa gttttggata aagcatggtc
    12601 tgaagtatct ccaaaataaa cttatataat ataaaaatat acagagtatt ataatataaa
    12661 acttttaaat ataaaacttg aaaggaagga aaaccttatg aagattagtt ctatgtttac
    12721 acctataaga attggttcaa tgacagttcc aaatcgtttt gtagttcctc ctatgggaaa
    12781 caattttgca aacacagatg gtactttaag tgaaacttct aaggcttatt atcttgagcg
    12841 tgctttaggt ggttttggat taataactat agaatctagt gttgtagaca aaaaagcaaa
    12901 aggaggtcct cgtaagcctt gtttatatga tgatagcaca atagatagtt ttaaaaatgt
    12961 aatagatgct tgtcatgatg caggttcgaa ggtatctata caattacaac atgctggttc
    13021 tgaaggaaat gaaaaagtag caggacatcc attaaaagct gcttctgcaa tacctgcatc
    13081 aaatggtcgt aatacaccac tagcaataac aacagaagaa atatatgaac ttatagaatc
    13141 ttatggagat gctgcattac gtgcacaaaa agctggtgca gatgctgttg aagttcactg
    13201 cgctcatgga tatctggtta gtagttttat atcacaaaga acaaataagc gtgtagatga
    13261 gtttggtgga tgttttgaaa atagaatgag attacctcgt cttatcattg aaaatatacg
    13321 taaaaaagta ggaaattctc tagcaatatt atgtcgtata aacagcactg atgatgttcc
    13381 aggaggaatt gatgtacacg atagttcagt aatagcagca tatctagaag attgtggaat
    13441 agatggactt catgtttcac gtagtataca tatacatgat gaatatatgt gggctcctac
    13501 gactttacat gctggattca gtgcagaatt agttactgaa ataaaaaaag ctgttagtat
    13561 accagtaata acagttggac gttatacaga gccacagttt gctgagttaa tggtcagaca
    13621 aggaagatgt gatttagtag cattcggtag acaaagcttg gctgacccag aaatgcctaa
    13681 taaagctaaa aatggaaaac tagacgaaat gataccatgt attgcatgct tacaaggatg
    13741 tgtaccaaat atgttccaag gcaaacctat agcctgtctt gcaaatccta tattaggaca
    13801 tgaagcagag cttaagccag ctgaaattag caaagaagtt cttgttgttg gtggaggtgt
    13861 tggaggtatg cttgcagcat gggtatgtgc taaaagagga cataatgtaa ctttagttga
    13921 aaaatctgaa gttcttggtg ggcaaatgcg tttagctgca tatcctccag ggaaaggaga
    13981 tattactaac ttagttagaa gttatatttc aaaatgtaat caatatggtg taaaaatatg
    14041 tactaataca gaagctacag tagaacttat aaaggagaaa tcacctgatg tagttattat
    14101 agctactgga gcaactccac ttgtacttcc tataccagga atcaatgatt caggattaat
    14161 tcatgctgta gaccttcttg atggtaaaaa atcttgtggg aaaaaagtac ttgttgttgg
    14221 aggtggaatg gttggatgtg aagtggcagc cttccttgga gaacaagaac atgaggttac
    14281 tgttattgag cttagagaag aagttggtgc agatgtaatt tcagaacacc gtaaattcct
    14341 tatgaatgat tttgaaaatt ataatataca taccattact ggtgcaaaag tttctaaatt
    14401 ttatgatgat ggtgtcgact atttacttac agatggcaca gagcatgatt taaaaggttt
    14461 tgattcagta gtacttgcta tgggttctcg taattacgac ccattaagtg aagttataaa
    14521 agaagttgta aaagaaactt atattgttgg tgatgcagta aaagctcgta gggcattaga
    14581 tgcaacaaaa gaagcttttg aagtagcatt aaatatataa ataataaata aatttttctt
    14641 tgtaaataac tgaataggtt atttgcaaag aaaatgataa tattgagagg agattatagt
    14701 tatggaaatt tcaggtagaa caggattatt tgcattaata ggtacaccgg ttggtcactc
    14761 taaatcacca gtgatgtata attatagttt taagaaatta gatttagatt atagatattt
    14821 agcctttgat atcacagtag ataaggtaaa agaggcactt ttagcaataa aaacttttaa
    14881 tataaaagga gccaatgtta ctatgccatg taaatctgct gttacagaat atatggatga
    14941 actttctcca gcagctcgta taattggagc atgtaataca attgtaaatg acaatggaaa
    15001 gttagttggt catataacag atggagtagg ctatgttcgt aatttaaaag aaaatggggt
    15061 agaagttaaa ggtaaaaaga ttacaattat gggtgctggt ggtgcagcaa ctgctataca
    15121 agtacaatgt gctttagatg gagcaagaga aatatctatc tttaatccta aggatgattt
    15181 ttataaaaga gcagagcaaa cagttgagaa tataaaaaaa gatgtaccag aatgtgttgt
    15241 taatctatat gatttagaag atacaaacaa actttatgag gagatacaat caagtgatat
    15301 attgacaaat gcaactctta ttggtatgaa gccttatgac aatgaaacaa atataaaaga
    15361 tactagtgtt ttaagaaaag atttagtagt aacagatgtc gtttataatc ctaagaaaac
    15421 aaaaatgatt gaagatgctg aagctaatgg atgtaaggct attggtggat taggaatgtt
    15481 attataccaa ggagcagaag cttttaatct atatactgga ttagaaatgc ctgtagaaga
    15541 agttaatgaa ttatgcttta aatagataag agtaatatct taaacaatga aaaaacatac
    15601 gtatttcaat atttttaata ataaattaca aattgtcata gtgggttttg cctcatagtc
    15661 cagttaaatt tgaagtttta actgactata ctttaaatcc attatgggga tgagtttatt
    15721 gtacacaaaa atgttaataa attaacaaga atgggagaaa aattatgaac aataaaaaat
    15781 attatcctac tgccatagcg ttgtattttt catatttttt acttggtatt ggaatttcaa
    15841 ttttaggaca atacaaacca gaattttcat caatgtgggg ggctaaaaca ttatcagatg
    15901 gaacattgga tgtaagtatt gttttagctg ttattgctgc actaggatta gggagactta
    15961 tatcttatcc atttgcagga cctatttcag ataagtatgg aagaaaagta agtggtctta
    16021 taggaaactt tctacatgca attttctttg ttggaattgt attttcacca aatttttata
    16081 tagcatatgt atttgcaata attggagggg cagcaaattc attcttagat acctgtgtaa
    16141 ctccttcttg tatggagata tttgctagtt taggaacaat agcaaatatg tttactaaat
    16201 ttacaatagc tttagcacaa tttttgcttc cttttataat tggatttgtt gcagcaaatt
    16261 caatatcatt taaagttatt tttatagtaa cagcgattct tattgtagta gatgcctttt
    16321 tgatagctgt gttaccattt ccaccagcta ataatgttat tgataacaaa ggcaaaacag
    16381 ttaaatcaga gaagatgaaa tttacaccaa caagtatagc tcttgtttgt attggattta
    16441 catgtacatc tacatttgta ttatggttaa attgtaatca ggaattgggt aaattgtatg
    16501 ggatggctga ccctacaaaa attcaatcct tttactcaat gggagtaata tgtgcagtat
    16561 taataacttc tttacttatt aaaaaatata ttaaaccaat aagaatatta gttatatatc
    16621 ctataatagc tttacttatg ctattagttg tatattttgt tcaaactcca actatttgta
    16681 tgttaggggg atttgtaata ggttattcag cagcaggagg tgttcttcag ttaacaacgt
    16741 caactgcaaa tgaaatgttt ccaactaata aaggaaaaat aacatctata gttatgattg
    16801 catctagtat agcaaactat gttattttaa atatagcagg tattattaca aaatcagggg
    16861 gagttaatgg accaaaatat gttgtattgt ttaatgtggc aataacattt gttggaattt
    16921 tacttgcatt gtttgtaaac atgagatatg aaaaagaaaa agtgtatgat tatgatgtct
    16981 aatattagta taatatatgc atagagaagt ctaaatctag gatagatttt tattaaaagt
    17041 aattaaaatt aataaggata ttatttacag ttataagata gggtatttac ataattaata
    17101 ataaggatat tactagaatt tcaatttcta gtaatatttt tttgcgtata aatgtaaact
    17161 tgatgaaata agtagtataa tcaatataaa ttacatcatg taaaggagga taatttggtg
    17221 gagaatgttt ttaagcgatt gcaggaattt aatggttatg atggatacaa agaatcattt
    17281 gaaatgaatt acctatgtat atatgaaagt ataccgttaa gagaacaagt agagcttgca
    17341 aataatctag tagatgaaat tttaaatatg tataaaagtg aatctaatga aatttattta
    17401 ttggagggta gtaattctaa gtctttaatt tgttatttcg aaatttttat gaaaaagata
    17461 aatactttgg taaaagaaat gattattgat gagaaatggt tatataaatt aactaaagaa
    17521 ttaatatata agagtaaaaa agttgagtat gtaaaactag ggttagtttt aagtgagaaa
    17581 tatctaaatg tagaaaactt aagagaagtt gtagatacat tttctaaatc tggagaatac
    17641 gtattttatc tgagtaatac tattaaaaaa cttgaatttt acaatacata tttatttaat
    17701 ttatctaaaa aggctacagg ttcaataaaa gtatttgcta ttgttaatat ggaaaattta
    17761 gattctaaaa taaattccta tctaattgaa gatggttata aggatacaaa gtacgaacga
    17821 ttattgatga actatattat ttcaattgta gatttgaatg aatatcttga aaaacgagat
    17881 ttagataaag aaaagataaa taatttagca cgtcttatat gcaattactt gctaagtgta
    17941 gaatttaaat atataggtaa taaattagaa ttagtgaatc gatttttacc tactgtagta
    18001 aattatggga ctaactttga aagtttatat tcaattttcc taatagctat aaacgtctta
    18061 aaagatgaaa atatagagtg caataagatt gaatttgaaa aagaaataaa tggtatcttg
    18121 ctcagtgaaa aatggaaaaa tatatatttt gaagcattaa gagatgctag tggaaaaaca
    18181 gaagatatta taaaaatgtc agaaatttat gatgtaaact tatcttttga tgacttgttg
    18241 ccatatctaa atagagatat tagagatttt gaggtatatt ggcatatatc taaaaaggga
    18301 acgacaagtt ctagattaaa attattaaat ttttttgaag agacgtttaa aatagatgat
    18361 ttaattggaa aaatgaaaga tatagaaaaa gataaattga ctcaagaata ttatgatgat
    18421 atgttatttt ttattgtgct taaaggaagt aaatcactat atcctgaagg aaagaatata
    18481 tctttaaaag gaatatttgg aaatataaat gaagtaagaa aagaatccat aaatatctta
    18541 aaaagataca gagagaaact atctcttgaa gaattaaaaa ttgttaaaga agcgtatgaa
    18601 aaagaaaaaa atgttatatt gaaggatgaa ttaagaagag tattatatga aagtaataat
    18661 ttaaaaaaag aatttgtaaa tattgaaaaa ataaaagtag atgaacatgg taaggatatc
    18721 tatttgactt caatagctgt tgctggttca aggtttagaa atagagaata tcttgaaaaa
    18781 gaattggaga aaagtaagat atactattta actagagaga aagataatct atatgatgaa
    18841 aaagctatta aaatagttgg tgaaactgga tatgtgatag gctatgtccc aagaaaagaa
    18901 aactatattt tgagcaattt attagatgga ggaaaactgc tgtattgtag agtaactgaa
    18961 tataatttat atgaagattg tatctacgca aatgtgtact tgagttataa agatgtaatt
    19021 gaaacagttg aaaactcttt aaagatggtt ttagataaga gtagaataaa acttataaac
    19081 taagacgttt atatattgct tagatgatgt aatcctaact gattggttat tttttctgga
    19141 attatggtgt attttatgag caatgctaat gaatataaaa taaaaaaaca attgttttgt
    19201 atatatgatg tatataaata tatttatata ttcatattta caaaacaatt gaaatttatg
    19261 ttattttata attttaaatt tagatggaat tttaacttct gctttatctt ttattttaga
    19321 agcttgtttt ttaactttat ttaataattc agattcatct acaactaata cttttctttc
    19381 cttcatcaac catttaccat cacaaatagt agagtctaca ttttcactag tcatagaata
    19441 aactatattt ccaattggat tatgcattgg taaacaatga attgtatctg gatttagaat
    19501 aatcatatca gctttttttc ctacttcaag actaccaatt tctttttcta gtaaagcaca
    19561 ttttgctcca tttatagtag ccatttctaa gacttcttca gctggtacga cagttgggtt
    19621 taaagttctt cctttatgta ttagtgatgt aagatacatg tccctcataa ggtccattct
    19681 attatttgaa ggagctccat cagtaccaat tgaaacaggt attccttttt ctaacatttc
    19741 aggaattgat gcaaacccta gaaccacttt catagctgaa cctgggtcat gagaaacttt
    19801 tacatcatgt aatctaaata aatcaatttc cttatgagta agccaaactg tatgagcagc
    19861 taaaagattt ggtcctaata cacctagttt atcaaggtat tcaactgttc caactccatt
    19921 gttttgcttt acaaatgaaa tttcatcagc tatttcagca atatgcatat gtatacctgt
    19981 attgtattta tcagctagtt ctttagttct aacaagtaat tcttcagttg tattaaaaat
    20041 agttcttaat gcaaaccata ttttaattct ctcattagat gtattattaa acttatggaa
    20101 taagtctaat tgagtgttga tttcctcatc agtagttttt tgccatgcct caggaagacc
    20161 atctcctgta tccataactg atttagctaa acaggctcta agtccagttt tatcaacagc
    20221 ttcaaccata gcatctacat attgaccacc agattctaaa aatgtagtga caccagattt
    20281 tatcaattca acacaacatg cagtagatga aatcaaggaa tcttcataat tgaagctact
    20341 ttcgtatggc caaattcttt ctctaagcca agttaaaagt acaacatcat ctgcaagacc
    20401 acgtcctaat tgttgagata agtgaacatg agtattgact agccctggca ttagtatttt
    20461 tcctttagca tcatatatct ctgcatcttt atctacaata ttaatatcaa acttaccaac
    20521 ctcttttata atattatttt caataagtat gcttccattt tcatatacct ctctatctgc
    20581 attcatagta attagatatg cattttttat aagtaattgt gacataaaca ccactccttt
    20641 tctatatatt tttatatcta tatattatga taataaattt tctatacaaa gttaatagca
    20701 aaatcaattt acatataaag tgattttgct atttataaat ttaaaataaa taagatttta
    20761 ttctatataa tttaaaatat tgacctagaa ctatttaaaa atattaactt agaactaagt
    20821 ttacaactgc aagtaaacct ataatccaaa gtgtaatact gattttatca gtttctttag
    20881 ctactacatg cattattata tatgaaataa atccaaagca taaaccaata cttatactat
    20941 aagttaaagg catcaataaa actgtaacaa aagcaggtac agctattttg ctatcagcaa
    21001 aatcaatctg ttttatgctt tcaaacatga atacaccaac cattacaagt gcaggagctg
    21061 cagcgtaagt aggaactata ccaagtaatg gtgtaaatat aagtgtagct aagaataatg
    21121 ctgctgttac tattgaagca agtcctgttc ttgcacctgc tgcaatacca gaagcagatt
    21181 ctccaaatgt tgtaacagta ctagttccta agaatgagcc tattaaagta gaagaaacat
    21241 ctgcatataa cattcttcct aaatctttta tttcaccatt ctcatcttca agacccattt
    21301 gtttacaaca agtaataaga aatgctaatg aatcaaacat gtctataaac atgaaagaga
    21361 atataggtcc aattaatgag aatttcaatg cacctacaat atctaattta aaagcaactg
    21421 gtgctatact aggaggtgtc gagattattg ctgttggcaa ctccacaaat ccaaatataa
    21481 cagatattat tgttgcaact actatactta tcaatattcc acctttaact tttttatatt
    21541 ctagtattgc cattaagata agagcaaaaa gagctattaa aactgttggt gtaaaatctg
    21601 caagtgaaac tatagtatct ggattagcta ttataacacc catatttttt aaaccaacaa
    21661 aagcaaggaa aagacctata cctgctgaag atgctactgc caattctcta ggaattgcgt
    21721 ctgcaattgt ctttcgaaga cctgttactg cgagtgtaaa gaagacacat ccagatagaa
    21781 aaactacccc aagacctgtt tgccatggga ttcctgcacc tataaccaaa gagtatgtga
    21841 agaatgcatt taatcccata ccaggagcta gagcaaatgg tgcatttcct aaaatcgcaa
    21901 atataagtgt accaatggct gtagctagta tggtaactgt tataagggcg gctttatcca
    21961 ttccagcttc agataatatt gctggattta cgaaaactat gtatgccatt gttaaaaatg
    22021 tagtaactcc acctattacc tctgttttaa ggctagagcc acgttcgcta aatttaaaca
    22081 ttttgtctaa aaagcttaat gatgaattat ttccacctgg ttgtacattg ttagtgtttg
    22141 gtaccatttt tacctccaaa aaactttaag atataaacaa aaaaatatat attcatatag
    22201 atagaataga acatatattc agagttgaca ttctattcat agaaaaccca tttaaggtga
    22261 gtttgtagaa actattagac cgtattacta atattatatg aacattttct atttgttttt
    22321 ctgttgtatt atatcgtaat attgtattat ttacaataaa tataataaaa ttattcgttt
    22381 ttttgtcgat tttataaaaa attaaggtta tttattgaaa aaaattactt tttccaataa
    22441 ttgaatatgt atgaatataa tgtcaattat tctaac
//
LOCUS       DAEOSP010000047        22454 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.047, whole genome shotgun sequence.
ACCESSION   DAEOSP010000047 DAEOSP010000000
VERSION     DAEOSP010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.047"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            22..1302
                     /locus_tag="KRH54_001453"
     CDS             22..1302
                     /locus_tag="KRH54_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBG8579120.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNLAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDV
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
     gene            complement(1565..2956)
                     /locus_tag="KRH54_001454"
     CDS             complement(1565..2956)
                     /locus_tag="KRH54_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG8579121.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(2967..4187)
                     /locus_tag="KRH54_001455"
     CDS             complement(2967..4187)
                     /locus_tag="KRH54_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBG8579122.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(4201..4989)
                     /locus_tag="KRH54_001456"
     CDS             complement(4201..4989)
                     /locus_tag="KRH54_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG8579123.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(5006..6280)
                     /gene="larA"
                     /locus_tag="KRH54_001457"
     CDS             complement(5006..6280)
                     /gene="larA"
                     /locus_tag="KRH54_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBG8579124.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(6398..7111)
                     /locus_tag="KRH54_001458"
     CDS             complement(6398..7111)
                     /locus_tag="KRH54_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBG8579125.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            7236..8495
                     /locus_tag="KRH54_001459"
     CDS             7236..8495
                     /locus_tag="KRH54_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBG8579126.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            8775..9341
                     /locus_tag="KRH54_001460"
     CDS             8775..9341
                     /locus_tag="KRH54_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG8579127.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKYLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(9542..10747)
                     /locus_tag="KRH54_001461"
     CDS             complement(9542..10747)
                     /locus_tag="KRH54_001461"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBG8579128.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(10850..10956)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11048..11416)
                     /locus_tag="KRH54_001462"
     CDS             complement(11048..11416)
                     /locus_tag="KRH54_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBG8579129.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(11468..12745)
                     /gene="obgE"
                     /locus_tag="KRH54_001463"
     CDS             complement(11468..12745)
                     /gene="obgE"
                     /locus_tag="KRH54_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBG8579130.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(12856..13146)
                     /gene="rpmA"
                     /locus_tag="KRH54_001464"
     CDS             complement(12856..13146)
                     /gene="rpmA"
                     /locus_tag="KRH54_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBG8579131.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(13160..13477)
                     /locus_tag="KRH54_001465"
     CDS             complement(13160..13477)
                     /locus_tag="KRH54_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBG8579132.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(13492..13803)
                     /gene="rplU"
                     /locus_tag="KRH54_001466"
     CDS             complement(13492..13803)
                     /gene="rplU"
                     /locus_tag="KRH54_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBG8579133.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(13904..15325)
                     /locus_tag="KRH54_001467"
     CDS             complement(13904..15325)
                     /locus_tag="KRH54_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBG8579134.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(15343..16044)
                     /locus_tag="KRH54_001468"
     CDS             complement(15343..16044)
                     /locus_tag="KRH54_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBG8579135.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(16046..17890)
                     /locus_tag="KRH54_001469"
     CDS             complement(16046..17890)
                     /locus_tag="KRH54_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBG8579136.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            18209..19402
                     /locus_tag="KRH54_001470"
     CDS             18209..19402
                     /locus_tag="KRH54_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBG8579137.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(19614..20696)
                     /locus_tag="KRH54_001471"
     CDS             complement(19614..20696)
                     /locus_tag="KRH54_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG8579138.1"
                     /translation="MKKIRKLATKLLIAMMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGEVVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQQGAILDEVYGNNLRPKIEMYEGVPCSLINSSINTIKFTSKNSNLDPS
                     KHTVTVIGFMNTPYGMVALAPDGVPDEYPFEYDKPFSLDFTGKNLSAAELSKKCKENN
                     VIIKVEIGETASNSGRGMVAYARYVN"
     gene            complement(21260..22051)
                     /locus_tag="KRH54_001472"
     CDS             complement(21260..22051)
                     /locus_tag="KRH54_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579139.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(22055..22384)
                     /locus_tag="KRH54_001473"
     CDS             complement(22055..22384)
                     /locus_tag="KRH54_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8579140.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
BASE COUNT         7225 a         3987 c         2506 g         8736 t
ORIGIN      
        1 taaagataaa aggagtattt tatgaacata cttggattaa tagttgaata taacccattt
       61 cataatggac acatatatca tttatctaaa tctatagaaa aaacaaatgc aacacatact
      121 gtagcaataa tgagtggaaa ttttctacaa agaggcgaac cagctttatt tgataaatac
      181 actagagcaa aaattgctgt cgaaaatggt gtagacttag ttatagaatt accaacttta
      241 tttgcttgtc aaagtgctga aattttttct catggtgcta taacaatttt aaattcacta
      301 aactgcataa attctatatg ttttggtagt gaagaaggaa atacagaaat tctttataat
      361 ctagctgaaa ttttagcaaa tgaaccaaat gaatttaagt tacttttaaa aaaatattta
      421 gatgatggtc tgttatttcc aacagctaga gctaatgcac tttttgatta tatacatcaa
      481 gacaaaaaaa ataattttat acaaaattat tcaaaggaac aattatcaaa aacattaaat
      541 tcatcaaaca acatactggg tatagaatac ataaaaaacc tcatccattt aaacagtagt
      601 ataaaaccat ttactatctc cagggtccaa tcagaatata attccgaaga aattgagagt
      661 gatatttgtt ctgctactgc tataagaact tcacttaaag aaaatacaga cctttttcat
      721 ttaaaaaatg ttattccaga aaaaacttat aacatcctaa atgaaaaaat agataatggt
      781 tttttaccta tgtttgatga cttcttcttt gatattttaa aaacaattat acttagagat
      841 atggacactt taaaagagta ttttgaagta aatgaaggca tagaaaacag aatctataaa
      901 gatatcttta catctgaatt tctacatgaa ctacaattag caattaaatc caagcgatac
      961 actttaacca aaatcaaacg aactctaaac aatatattgt taggtattaa aaaaagcgac
     1021 gtgcaactta taaaaaacat agatactata ccttatataa gaatacttgc ttttaatgat
     1081 aaaggtagag aaattctaaa agagataaaa aataaatctg aaataaaaat aataaacaag
     1141 ttttcaaata tatcattttc attagatgat acgattttta aaacactcat aagttatgat
     1201 ataaaagcta caaatatata taattcaata tattataaaa acaataaaga tttagtaaaa
     1261 ggccctatgg atttttatac tcatccaata tatgtaaagt agttttttaa aataactata
     1321 tttataaaaa tcattttatt aactctttat tatataagat acaatagtct tactttattt
     1381 atgtacacat gcttttagga tgacaacatc aacttaaata ttatgttact ttctaaattt
     1441 aagtaaatat tgccatccta aaaattttac aatttagctt cagatttaaa agtaaagttt
     1501 aaaatattat atttaaatct aatttaatat tttttgtttt ataaatatta ttaaattaat
     1561 atttctaata tattttccct ggatttaaaa tatttttagg gtcaaaagct aactttatat
     1621 ttttaattaa catcatataa gcatctgaat ttgattcatg taagtactct tttttagcat
     1681 atcctatgcc atgttcacca gaaacttgac cactaagttc tctagatttt ttatacatat
     1741 agtcaaaagt ttcttttaat cttattttcc atgtattatc atccatacca tcttttaaaa
     1801 tatatatgtg tagatttcca tctcctgcat gaccaaaact ttttattctt atttttaatt
     1861 tgtcttgaag ctcgtgagtg tatcttataa actctgctat cttatctctt ggaacaacga
     1921 catcacattc atccatttgt gtcgtagatg cctttatagc ttctaaaaat gcacctctgg
     1981 ctgaccatat agaatcgttt ctttcttgtg tatctgatat aaatacatct aatgcaccat
     2041 tttctaaaca taaattagct actttttcat attctttttc tatatcttca gtgctatttc
     2101 catcaaatgt aagcaaaaga taagcatctg atgtattatc tggaaacttt ttacctaaaa
     2161 attcttctgc tgccaatatc acatctcttt ccataaattc tatagccgtt ggtattgatt
     2221 ttgattttat tattttagga acagtttcta ttgccattga taaatctgga aatggtataa
     2281 gcaaacttat acttttcttt ggtaaaggaa gcaattttaa tatagcttta gtaactattc
     2341 ctaaagtacc ttctgagccc actaataaat cctttatact atatccagaa ctatttttaa
     2401 ccacctttcc acctacattt ataatttctc catttggtag tacaacttca agtcctctta
     2461 cataatccct tgtaacgcca tattttactg ctctcatacc acctgcatta gtgtttatat
     2521 tacctgcaat tgtagcactt ttttcacctg ggtcaggtgg gtaaaataaa tctctatctt
     2581 gaacatattg acctattgtc atcaataaaa cacctggttc tactgtcaat gttaaatttt
     2641 cataatcaac ttcaagtatt ttattcattt tacatagatt aatcattatt ccaccattta
     2701 tggcaacagc agcacctaca agaccagtac cttgacctct tggtgtgact ggtatattat
     2761 ttttatatgc gtatttcatt attttagata cttgttcagt ttcaagtaca tttactaaaa
     2821 cttcagggta cttctcaatc cctccaagtt catcatgtga aaaatcttca tttatatcac
     2881 ttccaactaa tacattttct tctccacaag tatctattaa aaaatctata tcttttttat
     2941 cgattaattt atacatttct atccccctat ttagctgctg atgccatata tttttgatta
     3001 ttatctttgt tattttctat tttttcaatc aatttaggaa taatatcata aatatcacct
     3061 attaaagcta aatgtgctac atcaagtata ggcgctttat catctttatt tattgctact
     3121 atatagtcag caccctgcat ccctgcaaca aattgaactg ctccagatac accacaagtt
     3181 attatcaatt taggttttac agttctacca ctaagaccta tttgtttttt agcatctatc
     3241 caacctgctt ctattacagg tcttgttcct gccacttgag cgtttaactt atcagctaat
     3301 ttatatatca tctccatatc ttcttgtttt tttattgctc tacttgccac aactatcact
     3361 tcagcttctt ccagaccaac ttctttattt ttttctttta cactcagtac cttaatttta
     3421 gattttagtt cttttttttc tactttacat aaagttatct ttcctgtagt attctctatt
     3481 ttttctggtg ctgaaaatat tttatatctt acagtggcaa attgtggtct attattaggt
     3541 gtgtttatat gagccattat atttccacca aatgctggtc ttatttggtc taaatcagta
     3601 tttgattgaa tatcaagtat tgtacaatct gctgtaagac ctgttctaaa tctagcggca
     3661 agtcttggag ctaatgacct acctaatgta gttccaccaa ctaatactat cgttggtttt
     3721 attttatcta taaagttttc tattgctttt gaatatggtt ctatcctaaa atctttaaat
     3781 tcatcttcat catatacaaa tacttcatcc accccataag aaatcaaatt actgcactta
     3841 tcttttatgt cttttcctac aaatacacaa tacacaggtt gttttatttt tgatgccatc
     3901 tcacgagctt tacctataag ctcgtacgtt acaggatgta tatttccatt atgatgttca
     3961 acatatactg caattcccct ccattcatct ttattaattt gtactttact actctctata
     4021 aattcaaatg cgccttttgg tccacttttg acacacaact tgcacatctt acagccagca
     4081 cttatttcta ctatacctga cttttcttct atagccccaa aaggacatat ttctaaaatt
     4141 ctctttaagt cagttatttt atcttgattt acaactatct ttgacattta ttactccccc
     4201 ttacaccaac tttaattcct ctaatttatt tacaagtgca aaacttagtt catctgcatt
     4261 tcctcttaca atatttttat ctgtatttgg ctttggagga aatattctta caacttgagt
     4321 aggagaacca ttaagtccat agttattttc atctttatct tcaaagtcat ctaagctata
     4381 tacaggtatt tctctatctt tagtattaag ttttaattta aatgaaggta gtcttggttg
     4441 aaaaatgtct ttttctacag ttattaaaca tggatattca atctcacata cttgtaaatc
     4501 atttggcata tctatttcaa caactatact ttcatcttta acttcaatta attttgtaac
     4561 gttagtaaca tgaggtatat ctaaaaattc agctacttca ggtcccacct gtgctgtatc
     4621 accatcagta gtttgttttc cacaaattat taggtctacc tttcccatct tcttaatacc
     4681 ttgtgatatg gtataggaag tagccaatac atctgctcct ccaaatctcc tatcacttat
     4741 taatgctcct tcatcagctc ccatcataaa actttcttca attaccttct ttgcctgagg
     4801 aggacccatg ctaagtactt ttagattcgc tttcttatct tcctttattc taagagctgt
     4861 ttctaaagca tataggtcat agggattcat ttttgaatct actccatctc tttttagtac
     4921 tcctgtagtt tcatcaactt ctactgaagt cgttccagga acttgtttaa tacatgtcaa
     4981 aatttccata aagtacactc cttttttatt taactataac tgatactcca tcaggtataa
     5041 ctactacttt gctctcctta ccaactatac tattcgccat ttctaaagct tctttaaagt
     5101 taaatgcatg tttcatatgc atttttttta tcatttcagg attacactta tctgttacca
     5161 ttatcacagt atatttagat aatattctcg ctagtatctg aaattcccat tggtctggaa
     5221 tcgtatctac tctatcgaca cttctaattt tatctaaaag ttgttttgga gaatctgcat
     5281 ttgctacatt ttcataaaaa gattgacctc catgaccatc attacatgct gaaatcatta
     5341 ttattactcc accgtcttta caagtagctt cagctgctgt cattccttta acagattgat
     5401 aaatattttg gtctagtgga tatccaccat ttgtagttac aacaatatca gattttatag
     5461 aatttacttt tgataactct gttacaaatc tacatccttt agtatgtgct ttttcgacat
     5521 caccagcaaa ggaagcaatt atttttttat ctttatctat tacaacattc aatatgaatg
     5581 ctaattttgc aactttagct gcatatacca tatcctcatg aataggatta ttattcaatt
     5641 ttccagttct tgaattatca ctatctataa agtcactaca atgattgtac attatagtct
     5701 ttgcagatgc gattcctgga agtacacttt ttctgccacc tgaaaaacct gcaaagaaat
     5761 gaggttctat aaaaccttct gctatcaaaa gttctgcctc atatgctagt ttgtttatat
     5821 ataaatctcc acctgaaggc aatatcccta ctttacacat agatttatca tcagtagata
     5881 tatgcataac tatatcttca ttatctacta tttcttctcc cattttataa attagctctt
     5941 cccttgtact tggcctatga aatccagttg ccactataat cttcacatca atttctggat
     6001 taacattcct aatccttctt aatataattg gcatagttat tctacttggt acaggtcttg
     6061 tatggtcact tgtaattatg actacattct ttttcccctt aaccagttct tccagagaat
     6121 tagagcctat aggattattc atagattctt caactatctc ttcttgagtc ttatgttcta
     6181 tataattatc cgattttgac tccaaaacac cgagaaaacg ttcatcaggt atttctaaaa
     6241 tcattccttg ttttgaatac ggtactttta actttaccat ttttaatctc ccattataat
     6301 aaactataaa attggtatgt ggtcatacca gttttacatt tatatgatag taccattatc
     6361 taccatttgc aagaataatt tattttataa caaactttta attttcattt tttctataat
     6421 ttttacttat aaaatccata tgttcctttg ctttctcaca tgcaagttct acatcatttt
     6481 ctacaactgc attgtatata gcttcatgct gtttaagcaa tatattttca ttaaaaggtt
     6541 ctgcgattat tttttctctt gcatcttcta taaatccatc aaataatttt gatgctgtca
     6601 taaaaagact ttgaattaaa taatttttac ttaatgttgc tagtttactg tgaaattttt
     6661 ggtctaataa caccagtact cgattattat cttttttatt agtttctacc ctcatttcat
     6721 ctattatatg tttcaattct ataacttcag aagaagttat tctctttgaa gcaagttttg
     6781 caatctcaat ttctaggatt attctaagct ctaatatatc ttcaagtgta ccattattaa
     6841 gtttaaaaat catagataaa ggttctatta aagttttttc tatatttgta catataaaat
     6901 ttccttcacc ctgtctgctt tctatcactc ccatagtctc taatacccta atagcttccc
     6961 ttatggaagt tctactcaca ttcatttgtt cagacagttc tctttctgat agtaatttat
     7021 cccccttttt taactctcca tttagtattt tatattgtat ttgctcaatt acttgttcat
     7081 aaactttttt actagctata ctattaaaca ttttttcctc caatatgata atcgttgttg
     7141 taatatatat ctattatata tcttaagcca ttacattcat atatttttat tacttttaat
     7201 ataatatctt ataactcaaa tttacaggtg atattatgaa aaaaaataaa attattgctt
     7261 tttttgctcc atctatcata gttggagttc tagttttagg gattatccta tacccaaatg
     7321 aagctataaa atctgcaaga gaaggctttt ctatatggtc taatgtttta gttccatcac
     7381 tactaccatt tataattgga gcaaacttaa ttgttgattt aaaaatagtg gatattatag
     7441 ggtttatcat caatcccata actagatttg tatttaatgt ttcaggaaag agcgctttag
     7501 cttttgctat atctacagta tctggttatc cagtaggagt aagtttagct tctgaattta
     7561 ggagtaatgg acaaatttct aagtatgaag cacaaagact tgtgtctttt tgttctacat
     7621 caggaccttt attcataata ggttcggttg gtacaggtat gttccaaaat tcaaacttag
     7681 gttatttaat gattctctgc cattatctag gaactatatt ggttggtctt ttctttagaa
     7741 attatggtaa tgaaaatctt ccaaagacaa aattggattt aaaaacaaat ataaaaaatg
     7801 taatcaacat tagaaattca agtgggaatg gtttttttgt tttatttgga aatgctgtat
     7861 ttaatggagt aaatacatta cttacagttg gtggatttgt aattgttttt tcagtagtat
     7921 ttaaaatact atctcttttt aatgtaatct cattaatagc atcagttata tatttgcctc
     7981 tttcattttt aggtgttagt aaagaactct gtcaagcatt tgtaagtggt ctatttgaaa
     8041 ttactatagg atgcaataaa gtatctagta taacaagttc tccagaaatt ttaagagcat
     8101 ctttagctag ttttttgata ggatttagtg gattatccat cctagctcaa tgttgtacat
     8161 ttctatcaaa aacggacata gatttaaaat tatacatact aagtaaattt gcgcatggag
     8221 ttttagctgc tatctttata tttatacttt atccaattgc aaactctgct gtgttggttt
     8281 ctagctttgc tgatacatac aacgttatat ataataattt aatctggttt tattatctta
     8341 gctattatga tacaattctt caaattgtag taataattta tctaatctca gccgtattta
     8401 ttgcaaaaaa acatatgaaa actttatcta caaatgggaa actaatgcga tataaaaata
     8461 cttttttcac taagaaattt tcacgaaaaa aataaaacta aaaataaaac taataaaact
     8521 taactaaata cttccatttt tttattatat ttgtcataat tttaataacc tttttaatta
     8581 gtttaacagc atagttattt catcacaatt taataatttt attagaaata tttttcaaaa
     8641 aattctcctt aatcacatat atcacacttg tataagttta ctgggaaatt tttccattat
     8701 ataatatttc atttatatta tataataaat accgacctaa acttaccaaa tcaaactaag
     8761 aggagtggtt gaaaatggca acaagaccta tagaaataga cgaatataaa aagataatgg
     8821 aactattaca tactggcttt acttatagtg aaaatggcgt tgaaaaaaga tttagaaaaa
     8881 atcctaaagt tgcattggca ctaatgttag aagctaattt aggtctaaga atatcagata
     8941 ttttaagatt aaagataggt aattttaaag gtaatatgtt ggaaataaat gaaaaaaaga
     9001 cgggcaaatt acagtataga ccagttaata aaaatataat tgagtccata aatgaacatg
     9061 caagaaagta taatcttaaa tcaaatgact acttagtaaa tataaagaca aaggctattc
     9121 aaaaacagtt gaggataatt tgtaaatact taaatctgta taatataagt acacatagct
     9181 ttagaaagct ctatgctact actcaatttg aaaagagtaa caataattta gagttggtta
     9241 aagaactttt aaatcacagc tctgtagcca ctacacagcg atatataagg gtaactcagc
     9301 aagctattaa tgaagctagt gaaaatttct ttattggtta attataacgt atacattttt
     9361 ctattatctt attaattcac cagatattaa aaaagagtgt ctcaaaatga actttttagt
     9421 tcatgagaca ctcttttttt ataaaatcaa atatattttt attatttcaa cactggaaaa
     9481 aaggcttatc tctctatttt gagacaccct attaaatttt cataattctt gcataaacat
     9541 attaatctat cttcaataat tttttagctg attcactgta attatttctt tttacaattt
     9601 ccaaaactct atcaatctca tgttctgact ttgcacttac aaaaatcgcc aattcttcat
     9661 catctataat atcataacta tctacatatt tatctaaaca ttctaaaatt ccatctttat
     9721 tattataccc accaaatcta actactgcac tacactcatc ttctataagt ccacttaaat
     9781 ccaatttccc ttttgaaaaa gactttatat ttgaacttct attatctatt atatctataa
     9841 tatctgcata aacagcactt cctgttgcac ctcttccagc tcccttacct acaaaagaaa
     9901 gttcacctac tgaatcacct actaaaataa ctacgttgaa ctcattatct atctttgaaa
     9961 gcatattatt attgtccact aatgctggtc taacaaatcc acttacttct cctgattcat
    10021 acttacttct agcaaccaat tttatcttac aatttaattt ttttgcatat tctatatctt
    10081 tttcatctat tgaagttatt ccttctaata ataaatcttt ccaatatact atttttccat
    10141 aagataaagt agacaatata gataactttc tagcactatc atatcctaaa acatctgcat
    10201 ctggattaac ttctgcaaat cccaactctt gtgcctgttt taaagcgaca tcatacggta
    10261 aattttcatc atacatcttt gataatataa aattacaagt cccatttaaa atagcgttta
    10321 tgctctttat attgttgcct tccagtgatt ctatcaatgg ttttaaaact ggaataccac
    10381 ctgccacaga tgcttcaaac ttgatgctaa cattatttaa gttagctaat tctataaggt
    10441 cactaccttt ttcagccagt aaatctttat tagctgtcac tacatgtatt tttttattta
    10501 acgctcttgt tacataatca aaagctggat ttaatcctcc aattacttca actagtatat
    10561 caatatcctt gtcaaatata tcttctatat cagtagttat cttatcaaag tacttcttat
    10621 acttgtgttt ttctttattt ttaactaaaa ttccaacaac ttctatatct ctttttgaag
    10681 tattattttc tattaaatca attaatccac ttcctactgt tccataccct aatattgcta
    10741 tcttcactgt aacatacccc catcattttg attatattaa attatttata tatataaata
    10801 taccaataaa aaaagcttga gtataaactc aagccatatg acgggtttat actcatcttt
    10861 cagcataaat gctgcaggat ttagcaccaa tgtataatta aatatactgg ttgccgggct
    10921 tcacagggcc agtccctctg ccactcttga taagattgct aatttaaatt tatgtttaat
    10981 agtataatac aatttcttcc aattgtcact atttttttaa cttatttccc taaaaggata
    11041 taaactatta tctaacagct cttttagttg gtttaccctt ttttgtaaca ttattatttt
    11101 tagtctttgc tttcgcctgt tcatgtcctg gaaaaagtat tttaggctct tcaatattcc
    11161 tcttgtaaat agtaaattta aaaccaatag cctgcacaaa ttcagctctt aaagcttcac
    11221 atactgcatt agcagtttct ttagtatcaa gacctgcatt ttctaatata gtaactttta
    11281 ctaattctct agttcttaac ataccatcta attgctctaa gaaactctct gtaacacctt
    11341 ctttaccaat ttgtgtagta ggctttaaag tatttgccaa agatcttaaa tacgcccttt
    11401 gttttccttt taacatattc tcacctctat aattctatac ttaaattatc tataatctat
    11461 atttaaatta attgtaaaac tcaaattcta attcataaat tttaaccaca tctccatctt
    11521 ctattcccat ttctctaagc ctatcaaaaa caccttgact ctccatagct ttttggaaat
    11581 attgaaggga ttccatatca tcaaaattta cagaatacat tattcttcta agtgctttac
    11641 ctgtaactac atacacacca tcttcaatat ctatacttaa ctcttcttca gtaccaatat
    11701 caagttctgg tctatacatt tcttcttctg aaactagctc cacatcttct acttctttta
    11761 gcattttaga aacataggct attacttcat ctatgccttg acgagttgct gctgacattt
    11821 taaatactgt atatcctctg ccttctaatt cactcttaaa cttctcaaac ttactctcat
    11881 cttctaatat atcaaattta tttgcaacta caacttgtgg tctctttgaa agcttttcat
    11941 tgtacaattt aagctcatca tttatcttgt caaaatcttc aatagggtct cttccctcta
    12001 atccagatat atctacaata tgtatcaata cctttgttct ctcaacatgt cttaagaact
    12061 catgacctaa tcctatgcct tcagaagcac cctctataat tccaggtata tctgcaagta
    12121 caaaactatc tccaaactta gtttgaacaa cacctagatt tggtgtaagt gttgtaaaat
    12181 gatagtttgc aattttgggt ttagctttag ttacaactga tagaaatgtt gatttaccta
    12241 catttggaaa tcctaaaaga ccaacatctg ctatcatttt aagttcaaga gttatccatc
    12301 tttcttctcc atctgtacca gattttgcaa aagctggagc ttgtcttact gcatttgcaa
    12361 agtgttggtt acctttacca cctataccac cttttgcaac tatagctgta tctccttctt
    12421 tttttaaatc tgctaataca agacctgtag cctcatctct tattactgta cctgctggta
    12481 cttttaatac taaattttct ccatttttac cagctctttt tcctttgctt ccgtcgccac
    12541 cattttgagc ttgatatttt ttctggtact taaagtccat tagagttcta agccccaaat
    12601 caacttcaaa tatgatgctt gctccacgac caccatctcc accgtctgga ccaccagctg
    12661 gaacgtattt ttctcttcta aatgctaccg aaccatttcc accatttcca gccttaacaa
    12721 atattcttgc cttatctata aacaaattct tcacctactt aattaaaaac ttttaattag
    12781 cattaccaaa aataccatta taaattcatc agtattataa gaaaaaggag tgcctcaaca
    12841 ctccttttat gtatattact cagctatagc tactggatat atactaactt tttttctctt
    12901 cttgtctttt ctttcgaatt taacagtacc atcaactaat gcaaataaag tatcatcact
    12961 accttttcca acattagttc ctggatgtat tttagttcct ctttgtctaa ctattatact
    13021 tccagcagtt actaattgac catcgaatct cttaacacct aatcttttag atatagaatc
    13081 tctaccattt ttagaagaac ctaccccttt tttagatgct aataattgta aattcatgtt
    13141 taacatatgg agtcaccccc tactttttta aaatttttat attttttggg tattcccgtt
    13201 taatttcttt aaccgctaat tgaaagtgat ttagtaataa ttgtgcgtct tttacatgtg
    13261 tatctttgac tatacactct atatgacctt cttctcctac tactttttca aattcaatct
    13321 tttgtaattt gtctaaagaa tttataactg caatagcatt agatgtaact gcagcacata
    13381 ctatatcata gcctatttca gcaaaatcag catgtccctt taaacaaaaa ccttgcaaca
    13441 agaaatcatc attataataa tatttaactt ttatcataac ttaaccaata actaagcgtt
    13501 tattttttct acaactatct tagtgtatga ttgacgatgt ccttgttttc ttctgtaatc
    13561 tttttttgct ttatacttga aaactataac tttcttagct ttaccttgtt caacaacttt
    13621 agcttgaaca gatgctcctt ctactactgg agaacctaat tttaattctc catctttaga
    13681 gcaagctaat acttcgttta aagtaacaac atcacctgcg ttagcttcta atttttcaac
    13741 gaataataca tcaccttcag aaactttata ttgctttcca ccagtcttta ctatagcgta
    13801 cattaaatac acctcctcgg cctcaaactc gccagtacaa ggtacttcat ttgaagattt
    13861 aaacctttac tgtgcggcat tacaattatt aattataaaa tatttacatt ttattgtcaa
    13921 ctattttatc gaaaataaca ttggttttat tgtgctttaa tgtactattt gcgtttaaag
    13981 aaatctttat attatatttt tcaccaattt tatcaattat atccatataa tattctttta
    14041 tttgttcaaa aataaaatca ttaaattcta ccattatgtt tttgtaaaca gtatgttcac
    14101 ttattctcat aatttctttt tcaatactat ctagtatata atgtacagat tttattgttt
    14161 gttcaccatc acaacatgga caagacatta aataatactt atcaatggat tctttttctc
    14221 tacgcctagc aacttctact aaaccaagtt ttgtcatacc taatacttca gcttttcttt
    14281 tatctttatt aaattcttct tctaggatgt gtaaaatctc cttttgatgc ttctttttat
    14341 gcatatcaat aaaatcgatt attattatcc cagctatgtc tctaattcta agctgtctta
    14401 caatttcttt tgcagcttca agattagttt tataaacggt ttcatcaagt ttaccagttc
    14461 caataaactt acccgtattt acatctataa ccgtaagagc ttctgttttt tcaattatta
    14521 aataacctcc actctttaac catacttttt tatttaataa tttttctatt ttagattcta
    14581 ttctgtataa atcaaataca tctctatttt cctcaagaca cagtttatca acataagtct
    14641 tatctatacc tcttaaaata gacttaagtt cagagtactt atcataacta ttggtaatta
    14701 tagattcaat atcatcattc acattatctt ttacatattt agttgcaaaa tctaaagact
    14761 tatataataa tttcggacct ataccaagtt tatattcttt taaaatgttt tcatacttta
    14821 atttcaactc ttctatatct ttctttattt catcatgagt gcaaccttga gcctcagtcc
    14881 ttattattaa tcctagattt tccttattta ctgccttagt aatttttttt aatctaaatc
    14941 gttctttttc atcagtaatt ttatttgata ttgtagttct atcattggaa ggtatataga
    15001 caatatacct tccagaaata ctaatctcag tgtttaattt tgcgccttta gttcctattt
    15061 cttctttatt tatctgaacc aaaatatcct gaccagattt tatagtactt ccctttttta
    15121 attgtaaata agcaagtttc ttaaatccaa tatctacaaa acaagcttct attcctggta
    15181 taactttttt tacgattcca cgatatatat tggatacagt cttcttattt aaattgtctt
    15241 caacaaaaag ttctgttaat ctttcatctt caagtactgc tgttttctgg gaccctatta
    15301 gggactcaat aactatcttt ttcacaactt cacctctcga gcttacatag gagtcactaa
    15361 ttccccatct tctaatacat ataaatctct tctcaatata tcaacatcta atgggtcaat
    15421 atcaagttca aacatctcta atatctttgg tataagtata ttagtattta aattagtttt
    15481 tgagcctgta gctatagtag cagtaagagt aatatgttca tcttctaaat ttaaaacatc
    15541 aaaggttctt atcataggtc ttatatctac ttcaaccatc ttaccttttt tattcttttt
    15601 tgttatgatt atttctttat tattcataaa gtcaattact tttcttttta caaactcttt
    15661 cgtcataggt cttttcaaat ctatattaaa caaatattct ccatagtcta tcacagaaga
    15721 aagagaaggt gtttgtctat caatttcttt tgctttgata aaatctatcc catctggaag
    15781 ttgaattgat actttattta aaaattcctc tactgataaa tcatcttctt ctatttcaat
    15841 atctacatac tcaccttgac tttctgtccc taaagccaaa gcattgccat aactcatttt
    15901 agggtgagga ttaaatcctt gagaatgtga taagtttatt tcagctcttc taaacgctct
    15961 ttgtaacaat ctctgaaggt caagatgtga tatatatatc atgtcgcctt cctttttaaa
    16021 ttttactctt attatcttac tcatattagc aaagccccct tcctatatca tgtatattta
    16081 ttccacaacc attgcagtta tgtctacaat cagaagtgat tttttctttt ttagcatttt
    16141 cgtcttctct aattaagaat tttttactaa tacctacatc tatatggtcc caaggaaata
    16201 cctcttcata gtttctattt ctatgtgcat aaaaatctat agaaagatta ttcttttcca
    16261 tagcttcttt ccatatatct aaattaaaat gttctgccca tccatcaaat ttagcaccta
    16321 gtctaaatgc atcatatata acttttccta ttcttctatc gcctctagct actactgctt
    16381 ccatcaaact agtctttgaa tcatgataat tgtattttat attgttattt cttaatttat
    16441 ttactaaatg tctctgctta ttaataactt cttctgtagt atcttgacca tgccattgga
    16501 aaggtgtaaa tggctttggt acaaatgtag aagtactcac tgtaacacta aaacttctct
    16561 ttagctttcc tccatttact tttctataaa tatcaataac tttataagct aatttagcta
    16621 taccatctaa atcttcataa gtctcagttg gtaaaccaat cataaaatac aacttaacac
    16681 tattccaccc catctcaaaa gctctttctg ttgcattttc caaatcttct tctgtaacac
    16741 ctttatttat aacatctcta agtctttgag ttcctgcttc tggagcaaaa gtaagtccag
    16801 attttcttac ttgttgtatt ttatcagcaa tttccattga aaaattatct aatctaaggg
    16861 atggaagcga aatcccaata ttattagatg catattcatc taccaaataa tcagtcaact
    16921 ctgataactt actataatca ctcgtactaa gtgatgataa tgatatttca tcatatccag
    16981 tattcttaac taatttatct aatatttctt taagtctttt aacactcttt tctcttattg
    17041 gtctgtatat catacctgct tgacagaatc tacatcctct agtacatcct ctaaataatt
    17101 ctagtactat tctatcatga acagtatcta tataaggtac tattagtttt tctggataat
    17161 ctacagtctc aacatctttt attatccttt tagtaggatt tttagtaatc ccttctctat
    17221 taggagttac actttgaact gtaccatctt cattgtactt gacatcataa aaacttggta
    17281 tgtatactcc ttctatgcta gatattttat ataaaaaatc ttctctagtc gttttatttt
    17341 tcttccattc tttatactca tttactactt ctaaattaac ctcttcacct tcacctaata
    17401 ttactacatc tacaaaatct gctaatggtt cggaattata agcacaagga ccaccaacca
    17461 ttataaatgg gtcttccaaa gttctttctt cttttaatat tggtatattg gctaaatcta
    17521 atatatttaa gatattagtg tatgaaagtt catattgaag tgtaaaagtg acaaaatcaa
    17581 agtttgttat aggctctctt gactctaatg caaataatgg tatacttttt tctctcatta
    17641 tattttccat atcaactgca ggagaataca ctctctcaca aaatacatct tcatccttat
    17701 ttattacatc atataatata tgactcccta agtgactcat accaacttca tataaatctg
    17761 gaaagcaatg tgcatatctt atcaattcac tattactagt atctttatga attgaattta
    17821 tttcatttcc aagatatcta gcaggctttt ctactttttt taagattttt ttcaagtcta
    17881 ctttattcat ttattaaaat tcctccaaac aagctcttta tttttattct tatttataag
    17941 taatattata cacgataaaa tattttctat caaaaatata actaaaatat tgaatttaaa
    18001 atacatatta ttcacaaaat agaatagcta aaaatattta agaatatttt ctattaaatt
    18061 tttattgaaa tacaattttt ttacaaagta tgatatatta taaacataaa actattgttt
    18121 tacatgactt atcatgacat cttttgtttc attcacctca aaatggggta tgttacttat
    18181 ataatgataa attttaggag ggttaaaaat gagtaaagtt tttgaagtaa aaaaagatat
    18241 ctactttact ggagtagtag atgaaggttt aaaagtattc gacataataa tggaaactga
    18301 gtttgggaca acttacaact cttatttaat caaggacgaa aaaacggttt tatttgacac
    18361 tgtaaaggca aattttaaag atgagttttt aagtaattta tcagaggtta ctgatatagc
    18421 taaaattgac tatgttgtaa ttcatcacac agaaccagac catgcaggta gtttaaaata
    18481 cttattagat ataaatccta atatagaagt ttattgtact aaagctgcta aactatactt
    18541 agatggtcaa ataaatagac cttttaactg tcatgtaata aaagatggtg aaattttaaa
    18601 tatcggtaaa agaaatctta gatttataac tgctccattc ttacattggg ttgatacaat
    18661 gtttacttac attgaggagg acaagactct tttaacttgt gatgcatttg gttgtcactt
    18721 tgcaagtgtt gatgcagagg tagtgaatag tgaagattat ctaaaatcag ctaaacacta
    18781 ctatgattgt atagttaagc catttgctaa acatgtttta agtgctgtag ataaggtggt
    18841 aggactaaac atagaatttg atactatatt gacttctcat ggtccaatgc ttactaaaga
    18901 cccaatggct gctgttaaaa gatatgttga gtggtctaca gaagctgtta atacaactaa
    18961 tcaaaatcaa gtttctatat tctacttatc agcttatagt aatactttag agatggctaa
    19021 aaagataaaa gaaggacttg ataaagaagg tgcaaaggct gaattatatg atttagaaga
    19081 tatgacatta acagaaatgc acgatacatt agtagtgtca aaagtaatat tactaggttc
    19141 tccaactata aacaaaacta tggttaaacc tatgtgggat ttattctctg ttatagaccc
    19201 aatggctaac caaggcaaga tagcaggagt atttggttca tttggttgga gtggtgaagg
    19261 tataactatg gctgagactt tacttaaatc tatgtcattc aaaatgcctg tagaatctct
    19321 taagaaaaaa ttcttcccta gtgaagagac attaaaagag tgtatggcat ttggtgcaga
    19381 atttgcaaaa ttagttaaat agccattaat caaacataat attatttaaa ataaatgatt
    19441 ttatatttaa aaataaatta aaatggagtt gtctcaaaat taatttttct aatctaattt
    19501 taagttcaac tcctttttta ttttctaaaa tgactgatat aatgataaat gcaccctttg
    19561 tgcgagggtg cattatttat aatatgttct atttgtacac tagtatattt gtattaattc
    19621 acatatctag cgtatgctac catccctctt ccagaattag atgccgtttc accaatttcc
    19681 actttaataa ttacattatt ttctttacac ttttttgata attctgctgc agaaaggttc
    19741 ttaccagtaa aatctaaaga aaatggctta tcatattcaa aaggatactc atcaggtaca
    19801 ccatctggag ctaatgcaac cataccatat ggtgtattca taaaccctat tacagtaact
    19861 gtatgctttg atgggtctaa atttgaattt ttgcttgtaa acttaattgt atttattgat
    19921 gagttgatta aactacacgg aactccctca tacatttcaa tttttggacg tagattattt
    19981 ccataaactt catcaagtat tgccccttgt tgatctgcta tcatagtgtg attcccaggt
    20041 ccaactggcg cagaaggctt agctggcgta ggtttcttaa cagttggcgt tggattctgt
    20101 gtctttacta cttcgccaac agctggatat gctttcttgt caaatggtaa tgggtgagta
    20161 agactcttgt atatgatgtc tgctcctgct gatttgttgg catcaatatc tacatgatac
    20221 aaacgtccat cctcaaaacg aataagattc cactgatggt catttgttgt actatgtaca
    20281 taataacatt caattcccat agcatcacat aaagctttca agctcctaca cattccagaa
    20341 caagcacctt ttttagtaac caatgtataa gcagtattgg ctccctctgc tgcgttccag
    20401 ttaacatatg ttgtgttgtt tttaatgtga ttataagcaa ccctaatctt agcatctgtg
    20461 gtcatgttct ttgtaatttt cttgttcata aagtctgctg ttgcttgtgc taccgaatta
    20521 agttgagctt gtgataactt tggaccatat atactgttgg ttccagctac ataacttttc
    20581 ggtgtctttg cagtaacatc tttagctgtc ataccataaa ctgttacact catacatgag
    20641 gttaaaacca tcattgcaat caatagttta gtagctaatt tcctgatttt tttcatatta
    20701 gcattctcct ttataatata ttttactttc aattccctac cattaattaa gttcatttta
    20761 gatttaaaat atggattatc aacatgtatg catagcaatt cacgtggtca tagaatattt
    20821 agatttcttg gaaaatctgt tctatatttc ctatatagtt cgtatacaat ccaactcaca
    20881 aacctgcatt tttgatatca tcacaataaa atgataataa ttaatatatt caaaaatgtt
    20941 ggtctgtaac aggtatgtca attttccaat tattggtaat ggcgagagtg aggagaattg
    21001 tcaataatac atttaaaatt aaaattgtat acacttcata cattctcccc taacaacatt
    21061 atacagatta taatttgtat attcaataca ttttttggaa agtataatta tatcaaaata
    21121 tgtgattatg tatagataca atccactatg gagaaatata aaagaaaaga gtataacaaa
    21181 caataaaaaa taagatgatt caaattttat tttgaaacat cttatttact ctacaaatta
    21241 agattatttg gtgaattatt taattattat tttttattaa agaaatttta aatcttaata
    21301 taaaatacgc tattatacat aataatccaa atgcaaatgc tactggaatt aacatctccg
    21361 attcaaaata aaagaaatat tctaagaaaa tatctggatt tactatattc ccattcttgt
    21421 ctaaaaaatc ttttccatta tatcctaaat gctgtataaa attacttatg ttattataat
    21481 ctgcttccct ctcatatacc atattagcat tcaatccaga agaacttaaa ttagtgtttc
    21541 catctttcat atctattaca tatagtctaa gagctttgac tttactatac ttatctttat
    21601 caagtatttt ttcaagttct aacttatatt caccaatctt tgtcatatca tgtctttctg
    21661 ccaattgctt tatttctcca tcaagttctt ttcctaaatt atacatatta tgttgtaaac
    21721 tattattgta ataccacata aatccagaaa ttaaaaatgc aattatactt atgtaaccta
    21781 atactttttt aaaccccatt acaattgatt tatgcctatc caatgataaa gatagttctt
    21841 ttattatatt acataattca tactgcattt catcaccatc atcaaatctt tcaattgctt
    21901 ttttacatgc ttcttcttca tctaatcctt ctaatttaaa ttcatttgca gattctataa
    21961 gatggcatct catttcctgt tttaattcta tagtactttt attgtcacat tttttatata
    22021 gtttatctaa atattcatct attagtttca taaattaatc cacctctccc aaaaattgat
    22081 tcataacctt tttaataaat ctccattctt gtttctttat ttctaaaaaa tctttacctt
    22141 cattagttaa attataatat tttcttcttc cacctgaact ttgttcattg ctataataag
    22201 atttaattaa atttttagat tccattcttt taagtgcaag atacattgtt ccttctttta
    22261 attcaaactc acttctttct cttacttgct ttgcaatttc atatccataa caatctttct
    22321 tttctaatat agaaacaatt aatatatcga tataaccttt taacacttct ttatttaact
    22381 gcattatgta ccaccgcctt ttcacctact actctataat gtatagtact tatatttact
    22441 ataataatac atag
//
LOCUS       DAEOSP010000048        21709 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.048, whole genome shotgun sequence.
ACCESSION   DAEOSP010000048 DAEOSP010000000
VERSION     DAEOSP010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21709
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.048"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            69..725
                     /locus_tag="KRH54_001474"
     CDS             69..725
                     /locus_tag="KRH54_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579141.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKIMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(1202..2473)
                     /gene="sleC"
                     /locus_tag="KRH54_001475"
     CDS             complement(1202..2473)
                     /gene="sleC"
                     /locus_tag="KRH54_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBG8579142.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            2718..3434
                     /locus_tag="KRH54_001476"
     CDS             2718..3434
                     /locus_tag="KRH54_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBG8579143.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            3513..4823
                     /locus_tag="KRH54_001477"
     CDS             3513..4823
                     /locus_tag="KRH54_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBG8579144.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            4923..5459
                     /gene="lepB"
                     /locus_tag="KRH54_001478"
     CDS             4923..5459
                     /gene="lepB"
                     /locus_tag="KRH54_001478"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG8579145.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            5625..6161
                     /gene="lepB"
                     /locus_tag="KRH54_001479"
     CDS             5625..6161
                     /gene="lepB"
                     /locus_tag="KRH54_001479"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG8579146.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            6171..6926
                     /locus_tag="KRH54_001480"
     CDS             6171..6926
                     /locus_tag="KRH54_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBG8579147.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            7000..8667
                     /locus_tag="KRH54_001481"
     CDS             7000..8667
                     /locus_tag="KRH54_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBG8579148.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            9481..10410
                     /gene="glsA"
                     /locus_tag="KRH54_001482"
     CDS             9481..10410
                     /gene="glsA"
                     /locus_tag="KRH54_001482"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBG8579149.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            10508..11071
                     /locus_tag="KRH54_001483"
     CDS             10508..11071
                     /locus_tag="KRH54_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8579150.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            11559..12395
                     /locus_tag="KRH54_001484"
     CDS             11559..12395
                     /locus_tag="KRH54_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBG8579151.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            12679..13674
                     /locus_tag="KRH54_001485"
     CDS             12679..13674
                     /locus_tag="KRH54_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG8579152.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKEYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(13931..14335)
                     /locus_tag="KRH54_001486"
     CDS             complement(13931..14335)
                     /locus_tag="KRH54_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBG8579153.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            15432..16346
                     /gene="queG"
                     /locus_tag="KRH54_001487"
     CDS             15432..16346
                     /gene="queG"
                     /locus_tag="KRH54_001487"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBG8579154.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            16452..18689
                     /locus_tag="KRH54_001488"
     CDS             16452..18689
                     /locus_tag="KRH54_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG8579155.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            18886..19938
                     /gene="nth"
                     /locus_tag="KRH54_001489"
     CDS             18886..19938
                     /gene="nth"
                     /locus_tag="KRH54_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBG8579156.1"
                     /translation="MKNVNLKEINEKDVIVEEDINLKGMDKKDVIEKEEVNKKSIDKK
                     DIIEKEEIDKKNVKKVEVQKGIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKST
                     KKGETEKKTKKSSSNKATQKTSKETKSKSKIKNESANEKDVNKILDELEKLYPDAKCE
                     LNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGL
                     YKSKSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDT
                     HVFRVSNRIGIVDEPNPQKTEFALMETIPKERWSHSHHVLIFHGRRMCKARNPECASC
                     PIKEDCNYYKELNETK"
     gene            20132..20602
                     /gene="trmL"
                     /locus_tag="KRH54_001490"
     CDS             20132..20602
                     /gene="trmL"
                     /locus_tag="KRH54_001490"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBG8579157.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            20638..21486
                     /locus_tag="KRH54_001491"
     CDS             20638..21486
                     /locus_tag="KRH54_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBG8579158.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
BASE COUNT         8564 a         2067 c         4039 g         7039 t
ORIGIN      
        1 agattgtaaa ttaaataaat ctttaataga aaggagaact atttatatat taaatagtat
       61 ttataaatat gaaaaaaggc gattttatat ggagtggagt tcttatttgt attattgctg
      121 tcttagcaat accaagtagt agagaagtat ttatggaagc aacaggtgca catccatata
      181 ttggaggttt tttaaagttt gctgtattag ctacaatggg agatttactt ggtgctagaa
      241 ttttgtataa ggattggaaa ataccagtag gagtatttta tagagcaata gtttggggaa
      301 taataggtgt agttaccaca tatggaatgt caatctactc atcaggtgta ggtcaagcac
      361 aagtggatgg tatgcttcca tttgagggaa tagcttttgc aaatgctcta tttaagagtg
      421 caactatgaa tatactaatg tcaccaactg tatttttatt ccataagatt atggactgtt
      481 ttatagataa aaaatatgag gttggaaagg ggattaaggt aaaacttaaa gatgtggtaa
      541 atattgtgga ctggaatgct taccttggtt ttagtgtttt aaaaactata ccatttttct
      601 ggataccatg tcatacaatt gtatttttat tccctgctga gtatagagta attgcttctg
      661 catttgcatc tatagcttta ggtctaatat tggctttagc taataagtct aaagttgcag
      721 tttaggttca ctgatgaaga taatagtatt atttaaatta ggaataactt tttaaattaa
      781 tattatttgt atttataata aataataatt tatataagaa gtaatattct aagtgatgga
      841 tagtgtttta aataataaga agtaatactc taagtgatag atagtgtttt aaataataag
      901 gagtaatact ctaaatgaga agtatcattt taaataataa gtgagatttt aaataataag
      961 taggtttttc gttagaatag cattttatta aacttaaaaa taaatataat attttaaaga
     1021 aatctaagtt gctaagcact gtttttaaga ttctaaaaaa acaagttata actaaaaaat
     1081 ggctgttaag actacaatac ttataagtaa tacttatggt atatattagt tcataacagc
     1141 catttttatt ttaaattaat aattttattc aataatttga gtttatgttc taaatcaagc
     1201 tttaaattaa aggatttaaa gaagctattc tagttgtagc tacataaact ctggatattt
     1261 cataccatgt tttaaaatct actactccgc tttgaggtaa accaaagatt ccttggaata
     1321 tcttaacagc gttttctgta tctgttccat agataccatc ttctataact tttggtacag
     1381 ctggataaga attagatata gcatttaact gattttgtat ggttctaaca tactgcccag
     1441 aagaaccaac ttgtaatgta gtaccaggga atgacactgg tacaccactt actataggcg
     1501 ctctttcaaa tactatctca tctccataga aatatcttag tatactctca tagtcatatc
     1561 cttggtcacc taagtcttta ctgccccatt gagtcatttg gtcaggacat tgggattttt
     1621 gtccatcaca atattgtgct aatagtggct gtcttgatgt aggaggtctt tttataaaag
     1681 tattaaaaat agcatctaca actacgttaa ttggttcaaa taagtttcta ttatttataa
     1741 acttatggtc gtatgctgtt gttgaagtta ttgtaaagtt atatccttta tttctatacc
     1801 attcagtaaa gactctattt aaagtaaatg atattatagc tattacattt gcataaattg
     1861 tctgttcagg ccaagttgaa tatatttcag atgatgctat gtttttaatg tactctttaa
     1921 atggaatcca atagtttgga gctgatgaat cttctggcaa accatcatga actactataa
     1981 attcaggcac tacagggtta tctaatacaa cgaaaccagt aggaggtggt aatggtttta
     2041 agttactttc tggaatctta ggaggataag taccatatag agtatgttct cctatgtcaa
     2101 aaatcaactc actttgtctt gagaaagaac gcttacttct tgttctagga gacatgggaa
     2161 ctccttgtct tgcttcaatt gtagctaaca actgtgttcc ttctatgaca actgtctcat
     2221 atccatctgc tatggcttcc actatatact ggctataagg tctaacatca gatggttgta
     2281 gagagtaatc tacatctggt gctggtaata ctaaaccagt cacttgacca gattcattac
     2341 ttcttaggtt ttggtataaa gttgatgagc tttggctacc attagacatt gaatatatgt
     2401 ttactactgc attttgaata ggtctattgt tagtagcatc aattatgctt actgttaaga
     2461 aaccatcttg catcaaatca ccctttcttt aaatgaattt agttccctta tatttcaaga
     2521 tatgaatata gcttaaaatt tgttaaataa tatagatata aatttaaatt tttttgagta
     2581 tttgagtata tttgtattta aataatttaa aataatttaa tgtatatttt agtatgtatt
     2641 taaaacacga ataagttaaa aataagtaga tgatataaat cataaataaa tcaaaaattt
     2701 tgaaaggaaa ataatctatg aagctagatt tatttttatt agcaatagtt cctatattga
     2761 taggaatgtt ttggataagg tctaaagata gatattgtag agaaccccta atccatctaa
     2821 ttaaattttt tttaataggt gcttttctaa gtgttataat tatactttta gaaaatttac
     2881 tgatgaagtt taatgtattt gaagggtatt cagaactaat atatgtatca tttgtagttg
     2941 caggacttgt agaagaaggt gtaaaagctc tgatactgat tcctgcccta attaaagaaa
     3001 aacactttac tgaaaagtta gatggtatta tttactctgt atttttagct ttaggatttg
     3061 caacaattga aaatatggtt tatatttttt ctgaaagtag aaatctagca ctgcaagttg
     3121 gtattaatag ggcagttatc tctataccag ctcatgtaat gtttgcaatt actatgggct
     3181 actatatttc taagtataaa tttgagggaa ataaaaataa aagacgtgag tatttgttta
     3241 tggcagtgtt aattccaatt ttattacatg gagtttttga tttcatattg atgatagaat
     3301 atagatgggc tattatatta cttatagttt atgtaataat tttgtggaag ataaatcttg
     3361 ataaacttga gaaatatatg aatcactcta aaaaagtgtt tttcggtaat cttaggaaaa
     3421 agaagaagaa ataaaattta aaggaaagac ataaataatt aaagtataat aaatgacaca
     3481 caagtaaagc agattgaagg gggattatag atttgacaga attaccaaag gaatttttag
     3541 aagatattga agaaatacta aaagatgaat atgatgattt tataaaaagt tatgaggaaa
     3601 gtaagactac tgggcttaga ataaatactt taaaaattga caagaaaaaa ttatttgatt
     3661 tggagttgtt tgatttgaat caaataccat gggcagaaga aggtttttat tatgatgata
     3721 aaattgacag acctggtaga aatccactgc atgaggctgg agcatactat ttgcaagagc
     3781 cttctgcaat gagtgtaata ccaaaggcag atattaaaga gggtgaaaaa gttctcgata
     3841 tgtgtgctgc tcctggtgga aaatctacat atatactatc taagctaaac gatacaggtt
     3901 tattggtttc taatgaaatt aatgctagta gaattaaggc tctaggggaa aatttagagc
     3961 gatttggagc gagaaattgt attataacaa acaccgattc taatagctta agaaagacgt
     4021 ttaatgggta ttttgataag gttattatag atgcaccttg ttctgggcaa ggtatgttta
     4081 ggaaggatga ggttgccata tcagattgga gttatgcaaa ggtagtagaa tgtcaatcca
     4141 tccaaaaaga gattataaga gatggatata aaatgttaaa gaaggatggc attttaattt
     4201 actcaacttg tactttttca agggaggaaa atgagcatgt tataaatgag tttatcagtg
     4261 aatatccaca tgcagaactt attgaaatgg aaagattatg gcctcataag ataaagggag
     4321 aaggtcattt tgttgccaaa ataaaaaaat tagaagaaga agaatgtaat gtcaaagaat
     4381 taaaaataaa aaatttagat aaagaaataa aggattatag ggagtttgaa aagaaatttt
     4441 taaaggttga tttttatagt aataaaaaaa ataaatttta tttaagagga gaaaatttgt
     4501 acctacttcc tgaaatatat ccagatacga aaaaactaaa agtattgaga tatggtttgc
     4561 atttaggggt tttaaagaag aatagatttg agccttctca tgcactatct cattacttaa
     4621 aagttgaaga tgtaaaaaat gttgaaaact tcagtgtaca gagtgaatca attcttaaat
     4681 atttaaaagg ggatgttgta aattctaatg atagtagagg atgggttttg gtttcggttg
     4741 aaggaatacc tttaggttgg ggaaaagaat cttctggtgt aattaagaat cattatccta
     4801 aaggtcttag aaaagtattt taaactatta gaaaaaagaa gtataatatt tatattgtag
     4861 gtttatggta gattattttg ctgtagattg acttaatatt taacacagag gagacgattt
     4921 tagtgggtga agcagttaaa aaagaagttg tagaatggat aaaagtgatt gtcatagctc
     4981 ttgttttggc atttgcaata actcgtttta tagtgccaac aatagtcaaa ggagaatcaa
     5041 tgtatcctac attagttgaa cgtgattatt tgatagttaa cagaattgcg tacaaggtag
     5101 gagagccaaa atacaaagat ataatagtat tcaaaaccga cttaacagag gaaaatggaa
     5161 agaaaaaaga tttagtaaaa agagttatcg gggttcctgg tgaccatgta aaaatacaag
     5221 actccaaggt atatgtaaat gataagttgt tagatgagac ttcctatata cataataatc
     5281 gtactgatgg agatattgat atcgtagttc cagaaggaaa attatttgca atgggagata
     5341 atagagaaaa aagtttagat agtagatacg atgaggttgg attggtcgac gagcatacca
     5401 ttttaggaaa ggttctagtc agattgtatc cattttctaa gataggaact attgactaaa
     5461 catataaaat ttggagcaat cgctgattag ctgaaatcac cagaccatac acaatagttg
     5521 tctatataaa aaagacaaca ccttatttag tagacttatc taaaagatag tgtttaagag
     5581 acattatcaa taaagataat ataactataa aggagaattt gtgaatgaat gaaactatta
     5641 aagaagagat tgtagagtgg ataaaaataa ttattactgc actttttttt gcatttatta
     5701 taactcgttt tataaaacca acattagtaa atggagaatc aatgtaccca acacttaaat
     5761 cacatgatta tttggtagca aacaggatga catataagtt atcagaacca aaatgtggag
     5821 atataatgat atttaagact gatttattac aagagaatgg aaggaaaaaa gagcttgtaa
     5881 aaagggttat aggtgttcct ggtgaccatc taaaaattaa ggattctaag gtttatataa
     5941 atggtaagtt attaaatgaa gtttcatata tacatgataa ttatactgaa ggcgatattg
     6001 atatggtgat tcctaaggga aaagtatttg cgatgggaga caatagagaa gttagtttag
     6061 acagtagata taaagaagtg ggattagtag atgaagaaaa tattaaagga aaagttattt
     6121 taagagtatt tccttttaca gatataggta tttttgagta ggtgatttgt atggcagttg
     6181 gtatagcgtc cttattgttt aatattgagg aggctttaaa catttgtgag agtataaagc
     6241 aaataactca tttagagatt ggaattgaca atatatctga atgtagtgag ttatgtaaat
     6301 ataaagagag aatttctaag ttaggattat ctatagggat acatttgcct atggaattaa
     6361 atacttgcga aaacatagag tatatacgaa attcatggat aaattttatt gagaagatag
     6421 aatttgaact aaaaggattt gatttaaggt attttaacct tcatttggga tatgttatga
     6481 caaatagagt tagaaagaat agagacaaat atttaggaaa tagtgtggat tttttagata
     6541 aattaaatac aaattcatat gtatgtatag agaatactta ttcaaaaggt ggggattttt
     6601 caaatattgg aaatatatct tatgattttg aatatatatt taagaggatt aaaaattcta
     6661 agatttgttt ttgttacgat actggacact gtttaattga tgaggatgct tatgtaaaga
     6721 acttaaagga taaaataaga ctgatacact taagtgataa tgatggtatc aatgatacac
     6781 atgttggtat aggaagagga attttatctg aagaggggat taaagaggtc ttaacattag
     6841 atgcagaata tttagtatta gaaataaatt acgaagacat tgaagatact ataagtaaat
     6901 tgaataatat tgtaggagag ggttaaatac ctctctttat cttgttgata tgcctcatta
     6961 attaaaattt atattcatca aaaggaagga gatgttgaag tgaaaaaaat aaacttaaaa
     7021 atagaaggaa aagataagga atattcatta gaagaaaatt cccctggaat cagattggga
     7081 gatatagcaa aagaattttg tgatgagcat aagggttata taacacttgc agttgtagac
     7141 aataaactaa aagagctaaa ttgtagagtt aagaaggatt gtgagataaa ttttttagat
     7201 actactaatg aagatggaga gagagtttac tttagagtca tgtcatttat atttgtaatg
     7261 gcatgtagag aaatcttttg ggatagcaga gttacaatag aacattcact atcagatggg
     7321 ctatattgtg aagttcatat agatagaaag cttaaagaag cagatgttga aaatataaaa
     7381 aacaagatga aagagatagt aaacaacgat tatgttatag aaaaaattga agttacaaga
     7441 aatgaagcta tttctatctt tgaaaataat gaaatgtatg aaaaggcaga gcttttgaaa
     7501 tacaaagagt atgatagtgc taaagtatat aagtgtagaa actatataga ccatttttat
     7561 ggttatatgt tgccatcaac tggttatata aaatcatttg atattaagct ttataatgga
     7621 ggcgttataa ttttaggacc tagtgaagaa gataagactc ttcctatgaa atttgtacct
     7681 caacctaagc tatcaagtgt atattatgaa gcagaagaat ggtcaagact tatgggaatt
     7741 gacaaggtag tatctcttaa taaaataata gaaaacaatg aatatggaga tataataagg
     7801 acatttgaag cattgcatga aaagaagtta tcccaaatag cggatatgat aaaggatggc
     7861 aataagagag tagttttaat agcagcccca tcttcatctg gaaaaacaag ttttgctcac
     7921 agattatcaa tacatcttag agtaaataat ttaaatccta tttcaatttc tttagatgat
     7981 tattttatag atagaaaaca tactccttta gatgaatatg gaaattatga ttttgaatca
     8041 atttatgcaa tagatttaga gaagtttaac ttagacttaa agaaactttt agctggtgaa
     8101 gaaattgatg atataagatt caattttaaa gaaggtaaga gagaatacac tggtaaaaag
     8161 ataaagatag attcaaatca gccaattata ttagaaggta tacatgggct aaatcctatt
     8221 ttaacatcat ctataccaga tgaagataaa tttaagatat acataagtgc ccttactcaa
     8281 ataaatttgg atgaccataa tcgaatccct actgctgatt tgagaatgat aagaagaatt
     8341 gttagagact ataattttag aggatatagt gctgataata ctattttaca atgggcatca
     8401 gttagaagag gagaaaagaa gaatatattc ccgtatcaag aggaggcaga tgctatattt
     8461 aactctgctt gtgtatatga attggcagtt ttaaaacgat ttgcgaaacc tttgttggaa
     8521 gaaataaagg aagataatcc tgcacatatt gaagctacta gattactaaa gtttttgcag
     8581 tattttgttg aattggacga tacttctgac ataccaggca tttcaattct tagggaattt
     8641 attggaggaa gtaaaatagt tgattaatgt gtgtattata gattgtttta tgaagattaa
     8701 atggcatagc aagtagtgtg attggtttat gttaagtata ttaagtgtaa agtggagaat
     8761 taaattgata cagattttaa agtttgagtt gatatataaa aaggcttgtt ttatactttt
     8821 gtatgaagca agctttttat tttaaatttt acttttttgt ttaatatttt gttagtttat
     8881 gtgattattt tttaaaagag taaaagagta aaagagtaaa agagtaaaag agtaaaagag
     8941 taaaagagta aaagagtaaa agagtaaaag gtgttgggtt agaaattttt ggtgttataa
     9001 aaatttaaat tactggatta ttagattgct ggattgctaa attactaggt tgttaaatta
     9061 ttgaattatt aggttgttga attactaaat tactaggttg ttaagttact ggattattta
     9121 gatagttaga ttactaggtt gttaaattat tgaattatta gattgttgaa ttactaaatt
     9181 actaggttgt taagttactg gattattaga tagttagatt actaggttgt taaattattg
     9241 aattattaga ttgctaaatt attaggttgt taaattactg gattattaga ttactaaatt
     9301 gctaggttgt taaattattg aattactaaa ttattaagct ctttaaacta tttaaattgt
     9361 ttgaaaaatg aattgattct tgtagaaaat aattttaaag tataattttt tttaacattg
     9421 ttttattata gtataaactt gtgctaaaat agaatatata acataatata agaggtgcat
     9481 atgaatacgt tagatgagac attattaaaa gaaattataa gttctaataa gaagtatact
     9541 aattatggac aagtcgctag ttacatacca gaattaaaaa acgctagaag aaatgattta
     9601 ggaatttgta tcatagatag cgaaaataat ctttatagtg ctggaaactg tagcacaaag
     9661 tttactatac aaagtatttc aaaaccaatt gtgcttgcta tggcacttat ggataatgat
     9721 tgggaagacg ttttttcaaa cgttggaatg gagccaagtg gggacccatt taattcaata
     9781 atgaaacttg aaattaatga cacaaaaaaa ccctgtaatc caatgataaa tgcaggggct
     9841 atagtaacca cttcccttat aaatgggagt tgtttagaag aaaaagaaga aagaatgctt
     9901 agtttcttta gaaagttagc aaaaaatgat aatataggaa taaactatga tgtctataag
     9961 tctgaaaaaa tgactggaga tagaaacaga gctatggctt acttattgaa aagtgatgga
    10021 tttatcagag ggaatgtaga agatgttcta gacctttatt ttaaacaatg ctcaattgag
    10081 atagattctg tagatttagc gagaattgga ataaatcttg caaattatgg agtagatata
    10141 gaaaatggtg agcatttaat gagtgaaatg gtttctagga ttgtcaagac atttatgatg
    10201 acttgtggaa tgtatgatgc ttctggggaa tttgctatta aagttggcat tcctgcaaaa
    10261 tctggagttg ggggtggtat aatggcttct gtgccgggac gcatggggat tggtgtttat
    10321 ggacctgcgc ttgacaaaaa aggtaacagc gtcgcaggag ttaaagtttt agaggagttg
    10381 tcaaataaac tcaaattaaa tattttttaa aatattaaga aaggattttt ttgacaacaa
    10441 catataattt aatatataat taaattgtaa agaaatatga attaaataaa ttttttagga
    10501 ggggtcaatg aaagaggtat tggtattacg agatttggag tgcataaagg ctattgcgca
    10561 tccacgtaga atagatattc taaaagcatt tgacaaatcg ccattgtcag caaaacaatt
    10621 atctcaatta ttggaagaac ctcatgcaaa aataaactac catataaaaa tgttatacaa
    10681 agttggtatt ttagaattag ttgaggaaaa aataaagtcg gggattgtgg aaaagtacta
    10741 ttatccaagt gcaaaaaatg tggttatagg gaataggata cttaattttt ctttagacaa
    10801 tggggaagaa aaagaagaat tgtacatatc aaaatttgaa aatatgagtg aagtttttta
    10861 taaggcagca gaggaagatg ttttagaaaa tgaaaatata gttgattatc atgatatttc
    10921 tcttactcat gatgaattag ttgaacttag tgacactatg aagtcaaaga tagatgaaat
    10981 tttaaataaa agacaacaca atgttgaagg ttctaagtat gatatagcta tggttatagt
    11041 tcctactctt gaggaggaat gtcctagtta gaaaattaat tatggattaa ttaaagatat
    11101 aatattagag attgaacatc aaaatttact taataatcaa aaaatagttt ttggtatggg
    11161 taaaacaatg atttttaatc tctatttttt tgttttctta tacctattgt atttgtaaat
    11221 aatgtgtata gtgtaagatg tatacactat ctaagaagtg cttagataat atacaaagta
    11281 tgtaagatta taatatttga aattgattaa aatgggtata attaaagtgt aaatattaaa
    11341 attaaaaatt aatataaatt aaatcaatgc tagaaatatg ttaaaagagg aatgagatta
    11401 gttgggatag aacatccctt agagcttatg aatttttagt gaaaaagtac tatcaaatag
    11461 gaatctccat cttcatatgt tggctaaatg tagttaaggt ggagtagttc aaaattaaaa
    11521 ttgagtacaa gtcgtagtac aaataaaagg agattaatat gattaaatta ggaacacatc
    11581 tttcaatagc aaaaggattt gctaatgcgg ctgagacagc tgtatatatt ggtgcaaata
    11641 catttcaatt ttttagtaga aatccaagag gaggaaatgc taaagaattt gatgaaaaag
    11701 atataattaa atttcaagaa attagaaaag aaaataattt tgcaccactg cttgcacatg
    11761 caccatatac tatgaatctg ggtggtacaa aggatgatgt atacgaattt gcaacaaggg
    11821 tgatagatga ggatataaga aggatggact cattaggaat tgaatacatg tgttttcatc
    11881 ctggaagtca tgtaggtggt ggtgtagatt ttggtataga tagaatagta aaaggattga
    11941 acaatgccat aaagggtgat gaaaatataa ctatacttct tgaaactatg tcgggaaagg
    12001 gaactgaaat aggttttaaa tttgagcaat taaaaagtat aatagacaaa attgagcata
    12061 aagaaaaaat aggtgtgtgt cttgatacat gtcatatttt ttcagctggt tatgatatag
    12121 tgaatgattt agatggggtt ttggaagaat ttgataaggt tataggcata gaaagattga
    12181 aaactgtcca tctaaatgat agtatgatgc catttggaga caaaaaagac agacatgctc
    12241 ctataggaga aggaaagata ggattaaaag cattaattga ttttatggaa catcctagtc
    12301 taaaacattt accatttttc ttagaaactc cttttgatga tgaaggtcac aagagagagc
    12361 ttaaaatgat aaaagaaata ctatctagta aataaacctt tattaaaaat atgtgaaata
    12421 ctccgccctc aacataatta gggtggagat ataatcatga aatgtgataa tttaaattaa
    12481 tggataggaa taacaaaaat tgtatacaaa ttaactgggt ataaagtatg attatattat
    12541 gaatagactg atataaaata taattatatt atgaatagta tgtaaatata ttgcaaagat
    12601 tctcataatt tgttgtataa ttaaagatga catatttata ttatataaaa aattattaaa
    12661 agttaggtgg ggaaagatat gagaaattta ggcaatacta acatgaaaat aaagagagtt
    12721 ggctttgggg gcattccaat acaaaggatt actcaagatg atactaactt agttataaat
    12781 gagttagaaa aacaaggaat aaactttatt gacagtgcaa gagggtatac aataagtgag
    12841 gaagcaatcg gaattgccat tgaaggaaaa agagataaat ttttcttagc tactaaaagt
    12901 atgtccagag attatgattc aatgaagaga gatgttgaaa taagtttaaa taattttaaa
    12961 actgatttta tagatttata tcaattccat aatgttaaag aagaggagta tgataatctt
    13021 tttaaagata aaatggctta tagtgccctt ttagaggcaa aggagcaagg taagataaaa
    13081 catattggta taactagtca caatttaaat actatagaaa aagctataga agatggaaaa
    13141 tttgacacta tacaattccc ttacaatata gttgaaggac aagcagatga agtgtttaaa
    13201 aaagctcatg aaaaaggaat tgggataata gtaatgaaac ctttagctgg aggtgcttta
    13261 gacaatgcaa cactggctat taaatatata ctatctaaag agtacataga tgtagttata
    13321 ccaggtatgg agagtgttga acaggttaga caaaatgtcg ctgtacttga aaatcttgtg
    13381 ctagatgaaa aagataataa ggaaattgaa gaaataagaa attctttagg taaaaaattc
    13441 tgtagaagat gcgaatattg tatgccttgt gctgtaggaa taaatatacc attgagtttt
    13501 ttatgtgaag gttattatac tagatatgga ttaaaagagt gggctaaaga aaaatatgag
    13561 gttatggatg ttaaacctac tgagtgtatt gactgtgggc tttgcgagag tagatgtcca
    13621 tatgaacttc ctataagaga aatgcttaag actgttgttg aaaagctagg atagatttta
    13681 tttattgaaa tcggtgtatt aaatttactc cgatttcttt ttattagatg tggtcttata
    13741 tacaaatatt aaatttacat agttatagct attatataaa tttaatattt gtttaatact
    13801 tattaaatat ttacttaatg ctttttaaaa acatttgttt gagtatagac ataacacaat
    13861 tattattgtt gtattagtat agtttcaaat actggagtat caatataatg tcgaataaca
    13921 cataaaatct ttatttttca aaaatattat aggatatatc gtatttagat actggataaa
    13981 gttcaatttc aaccctaggg ttatctttat ctataaaatc aaataaaagt actggcttaa
    14041 gttgtttatc attttttatt attccacttt tttcaatacc atcacaaata ctttttggat
    14101 aattatgtaa atcccccatt ctcttatttg ggaagaataa tttcaagact gttattagat
    14161 tttcttcaac agtttcccct tgatactgtt ggtttataaa acctgcaatc atgttttcgt
    14221 atgcaagata cttagaatat tttccctttg aaggcatcca tgcttgacca tttatgttat
    14281 gaagcttaaa attagattta ctaataggtc tacctggaat tgttatttta atcaaatttt
    14341 tcaccatcct tatacagtat ttattataat ctgtatatga tttaaaagca aattgttatt
    14401 taaagtgttt tacagagctt ttttgtaaaa ttttccactt tagatgtagt aatataagat
    14461 aagtattttg cttacaaatt ttaaaatttg atatatgatg gacatattca tattagacta
    14521 ttataagacc tttataatat taataagtat agtttagtaa agagagtaaa aagaaatcca
    14581 tataagtcaa gtaaagactt aataaatggc aatgtaaatg ggatattaat tataagaaaa
    14641 aatcaatctt tttgcttaac tatagaaaaa atgtcaaaat ataactaatg atatttgaag
    14701 ttttagagtt tatattaaaa ggtaagaagg ttaagtgggt aaaaaaggtt gaatagatta
    14761 aaaaggctaa gtatctaaaa gagttaagta ggtaaaaaag ttaaataagt aaatgaaaaa
    14821 ggctaagtat ctaaaagagt tagtaggtaa aaaagttaaa taagtaaatg aaaaaggcta
    14881 agtatataaa ggagttagta gataaaaaaa ttaagtatct aaaagcgtta gtagataaaa
    14941 aaattaaata aataaaaaag actaagtatt caaaagagtt agtaggtaaa ataattaaat
    15001 agataaaaag actaattatt taaaggagtt aagtagataa aaaagttaaa taagtaaatg
    15061 aaaaagacga agtatctaaa agagttagta aataaaaaag atgaagtatc taaaagagtt
    15121 agtaaataaa aaagatgaag tatctaaaag agttagtagg taaaaaacta aataggtaaa
    15181 aagatgaagt atctaaaaga gttagtaggt aaaaaattaa ataggtaaaa agacgaagta
    15241 cctaaaagag ttagtaggta aaaaaattaa atagataaaa agacgaagta tctaaaagag
    15301 ttaagcaggt aagaaaattg aatagctaaa aagattaagt atttaaaaga gttagtagct
    15361 agaaaaggtt aaaaagacta aaaaaattaa ataaatttaa aaagtcaatt agattaaaaa
    15421 ggaataattt tatggataat aaggaattaa aagcattttg taatttaata ggattaaact
    15481 gtgtaggagt agcaggggta gataagtatg ataatttaga aaagatactt aaggacagac
    15541 aacaaaaagg ttatttaact ggtatggaag aaccaataat tgagaacaga attaacccaa
    15601 gacatataat gaaggatgct aactctataa tagtctgtgc atttccatat catataaata
    15661 gacaagaaaa gaataaaaat tcaaatttat caaaatattg tcatggaaag gattatcaca
    15721 ttgtagtaaa ggacttttta caacaaattt gtgattatat ttctaaaaca gtaacagatt
    15781 ttaaatacaa gttatttgct gataatggac cattagttga caggtattta gcttatttat
    15841 caggaatagg atattttgga ataaataata atatcataac tgatgaatat ggttcttacg
    15901 tatttatagg gtatatagta aataattata aatttgaaga agataaacca ctagaaaaaa
    15961 gttgttttaa atgtggtaag tgtgtaaaat attgtccggg aaatgctata ttgggaaatt
    16021 atgatatgaa ccccaaaaaa tgtttatcat atataactca aaaaaaagga gatttgtcag
    16081 agaaagaaaa agttgtttta aagaatggca aaaaggtatt tggatgcgat atttgtcaag
    16141 aagtatgtcc acataatgtt gaaataccaa caactcatat tttggaattt aaagaaaata
    16201 taatagacta tttggattat gatgagatac aaaatatttc aaataaagaa tttaaaagga
    16261 gatatggaga tagagctttt agctggagag ggagaaatat aataaagaga aatatggaaa
    16321 taattttaga taagtatgat gactaataat aagagtctgt ttcatatact ttaatgtgta
    16381 tttactttaa cacttgttgt gtaaaagtca tattatatca ttaaagttaa atatggggag
    16441 gtgggggaat attgaaatat aataatacat ctgaaataaa tccactagat tatatattgg
    16501 gtcaagaaag agctgtagaa gctatggaat tagggttgaa aataaataat ccagcgtaca
    16561 acatatatat agctggtgaa cctggaactg gtaaaagtac ttatgcctta aaggttttaa
    16621 atgagtatgc atccaaaaaa aatacgcata aagattggtg ttatatatat aattttgaaa
    16681 accctagaga gcctattata gttgaattag aaaaggggtt tggaagagaa cttaaaaaag
    16741 atatggagaa attaattgaa agtcttttag aagattttaa aaatgcgttt gaaagtgaaa
    16801 attttgaaat agaaaaaaat aaacttctgg atgaatatga aatagaaaaa gatatgcttt
    16861 tgaagcaaat taagaaatat ggagaagaaa aaggatttaa attaaaacag agtaaaatgg
    16921 gaatagtttt tatccctttg aaagaagagg aagatgaaag tgatgagtct gatgaagagt
    16981 tttacaaagc taaaagagaa ctagaaaata tggcgataca ggtcgtatat aaaattagaa
    17041 acttagaaga aatagctgaa aaagcagtgt tagagcttga agaagagata gctaaacttg
    17101 tagtagaacc tcatatcaag agattgtgtg aaaaatatga aaattatgat aagattcaaa
    17161 cttatctgga aaatataaag aaagatataa tagaatatat gtatttattc tatttagatg
    17221 aagaggagtt aaaagataag tatgataaag aacattttat taaatataaa attaacctat
    17281 ttgtagacaa tggaagctca aagaataaag aatcagcacc tgttgtagta gagataaacc
    17341 caagtccagc aaatctattt ggaaaggcag agtatgatta tgcaaatgga aatataaaga
    17401 ctgatttcac aaaactatta tcaggagcat ttcatagggc taatggtggt tatttagtac
    17461 tttatgcaga ccaattacta aaatatacaa tgtcttggga aattcttaaa aagactttgc
    17521 agactaaaaa agtaatattg gaaacacaaa cagcaattaa accagaaaat atgccattgg
    17581 atgttaaatt agtactgatt ggaagccatt atatatatga tattttatat agatatgatg
    17641 aagattttga aaaatatttt aaagtatttg tagactttga tagtgaaatg gataaaaatg
    17701 aagataatga agaaggaata gcaagattta tagcatacca atgtgataag aataatttaa
    17761 ggcattttac taaatcagca gttgaagagg ttattaaatt tagcactaga ttatctggtg
    17821 atttagaaaa actatcaact aagtttaata aaataatgga agttgtaata gaaggaagtg
    17881 cttatgcaga gtttagaaat tcggagtata cagagcagtg tgatgttaaa aaagcaataa
    17941 gtgaaaagag aaaaagaatc aatagagtgg aaacacatat ggatgaatca attgaaaatg
    18001 gatttactct aatagaaaca gagggaagaa aagtaggcgt gataaatggt ctgtcagtct
    18061 taagtacagg agaatactct tttggtagag cttcaagaat tacagcgacc actagcccag
    18121 gaagtaaagg catagtaaat atagaaagag aagtaaatat gagtggctct atacataaca
    18181 aaggagtact tatattaggt ggctatcttt ctgagaattt tgcacaggat ttactattgt
    18241 cactaaatgc atatgtttgt tttgaacaaa attatggtgg aatagatgga gatagtgcat
    18301 cttctgcaga attatatgta ctattatcat ctcttagtgg agtatcaatt aaacaaaata
    18361 ttgctgttac aggttctata aatcaaaagg gtgatataca agtagttggt ggtataagtg
    18421 aaaagataga aggattttat tctatatgta aaactaaagg gttaaatggt gagcaaggtg
    18481 tcataattcc tagaaaaaat caaagaaatc tcgttttatc agatgaagtt aatgatgcag
    18541 tgcgagatgg aaaatttaag atatatactg ttgaaagagt agaagaggct atagagatat
    18601 tgacagatgt taaatttgaa gaaataaaga aattggtaaa agaaaaatta atatctttca
    18661 gcaagataca aactgtgtca aaagaataag tttattatag aatttaatgg gtatttatgt
    18721 ataataaaaa gcaagaactt taataggttt ttgctttttt tgtaaatata tataaattaa
    18781 attcttattt tgtatgtttt atatattgat gtttattttt tgttgatatg attaaactat
    18841 ttataaatag cgttttataa attagtattt taaaggggga taattatgaa aaatgtaaat
    18901 ctaaaagaga taaatgaaaa agatgtaatt gtggaagaag atataaatct aaaaggaatg
    18961 gataagaaag atgtaattga gaaggaagaa gtaaataaaa aaagtataga taagaaagat
    19021 ataattgaga aagaagagat agataaaaaa aatgtaaaga aggtcgaggt tcaaaaagga
    19081 atagataaaa tgattggtaa aaaagatgag ataattgatg agaaagatat agagaatttt
    19141 atggatattg aagaagataa aaaaagtatc aaagtaagta aaaaaagtac taaaaaaggt
    19201 gaaacggaga aaaaaacaaa gaagagtagt tctaataagg ctacacaaaa aacaagtaaa
    19261 gaaactaaaa gtaagtctaa aattaaaaat gagtcagcaa atgaaaaaga cgtaaataag
    19321 attttagacg agttagaaaa attgtatcca gatgctaaat gtgagttaaa ttatgggaca
    19381 gcctttgaac ttttaatagc aactatttta tcagcacaat gtactgatgt aagagtgaat
    19441 aaagttactt cagagttgtt taaaaagtac aacacagcta gagactttgc taatttgagc
    19501 attgaagaaa ttagtaaaga gataaagagt tgtggtcttt acaaaagcaa gtctcaaaaa
    19561 ataaaggata catctgaaca gctatgtgaa ttatatgatg gtgaagtacc tgatagctta
    19621 gaaaagctta taaaattacc tggtgttgga agaaaaacgg caggggttgt acttagtaac
    19681 gcttttaatc atccagcaat agctgtagat actcatgtat ttagagttag caatagaata
    19741 ggaatagtgg acgagccaaa tcctcaaaaa acagaatttg cacttatgga aactatacca
    19801 aaagaaagat ggtcacactc acatcatgta ttaatttttc atggaagaag aatgtgtaaa
    19861 gctagaaatc cagaatgtgc aagttgccca ataaaagaag attgtaatta ttataaagaa
    19921 cttaatgaaa cgaaataact tttagaaagt aagttgtaca agagggattt tctgatataa
    19981 taatacttgt gaaaatgtta gataaaaaac tattatatac atgaaataaa aattattatt
    20041 aacattattt tatataatag tataatatta ttgttattct gtttataaaa taaaatttga
    20101 taaataaaag caatacttag ggaggattaa aatgtcatta aatatagtat tagtagaacc
    20161 agaaatacct caaaatacag gaaatataat aagaacctgt gcagcaacag gttcaacttt
    20221 acatttagta aaaccactgg gtttttcatt ggatgataaa cacctaaaaa gatgtgggct
    20281 tgactattgg gatatagctg atatacaata ttacgatagc tttgaagaat tacaagaaaa
    20341 gtatccaaat agtaaatact ttttttctac aacaaaggct aaacagtcac attcagatgt
    20401 gaaatatgaa gaaaattgtt ttatagtatt tgggaaagaa acaaagggat taccagaacc
    20461 tttgttgaaa gcaaatatgg atacttgtat tagagtgcct atgttggatg ttgaaaaagc
    20521 taggtcatta aatttatcaa attcagttgc tattgtagtg tacgaggctt taagacaaat
    20581 aggataccca aatatgaggt agttttaaca agcctaaatg agaaaggagc atacttaatg
    20641 aaaaatctaa agataatctg tgacagttta tcggacgtgc caaaagattt gttagataaa
    20701 tatgatatag atatagtttc attaacagtt atactggagg aaagagaata caaggataga
    20761 attgatatat ctggagatga attttataaa aggcttagag aagaaaatgt atatccaaaa
    20821 acttctcaag ctacttatgc ccaatttaag gaaatttttg agaaatatac aaatgaggat
    20881 agagagatac tttatatagc agggtctgcc acagctacag gtacgtatca aagtgctgta
    20941 atggcaaaga atgatgtaga tggtaaaata tatacatatg actctaactt gctttgtatg
    21001 ggaactggtt tacttgtaat aaaagcagga gaacttgcaa gtgaaggaaa gtctgttgct
    21061 gaaatattgc ctgttttgga tgaattaaaa gagaaggctt ttgttatatt ttcagtagat
    21121 acattggaat atttacaaaa gggaggtaga atatcatcta ctaaggcagc aataggaagt
    21181 atattaaata taaaaccgat tcttgaaata agagatggat tagtttcgca aatatctcaa
    21241 gtaagaggaa agaagaatgt aatttctaag atgatggaat tgataaaatc aaattgtggt
    21301 gatgatttat cagaccaaat tgtgtatata ggatatagtg atgattttaa ggaaaaggaa
    21361 caattatcta aggttatgca agatgaatta agacctaaag aaattagatt tttccaaatt
    21421 ggtgtgtgta taggtgcaca tgctggtcct ggtgttactg gtatcatatg cttaaaaaat
    21481 aaataatata tttttggatg acattattta tttttttatt aaattaggat agaatattaa
    21541 ttattgagat gttaaattgg atttattttg gatgaaatta agtgttgata tattagattt
    21601 agtttgtagg gaagtgtgta aatagataag aatctattgc acactttttt atagtaagat
    21661 ttattaaaaa aatttcaaaa tgtacttgta aattgtatat aaaaaaatt
//
LOCUS       DAEOSP010000049        21160 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.049, whole genome shotgun sequence.
ACCESSION   DAEOSP010000049 DAEOSP010000000
VERSION     DAEOSP010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21160
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.049"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            156..1580
                     /locus_tag="KRH54_001492"
     CDS             156..1580
                     /locus_tag="KRH54_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBG8579159.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            1615..3744
                     /locus_tag="KRH54_001493"
     CDS             1615..3744
                     /locus_tag="KRH54_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG8579160.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKSFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            3774..4196
                     /locus_tag="KRH54_001494"
     CDS             3774..4196
                     /locus_tag="KRH54_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8579161.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4215..5366
                     /locus_tag="KRH54_001495"
     CDS             4215..5366
                     /locus_tag="KRH54_001495"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG8579162.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEGILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      5472..5573
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5756..6328
                     /locus_tag="KRH54_001496"
     CDS             5756..6328
                     /locus_tag="KRH54_001496"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBG8579163.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6392..7042
                     /gene="fsa"
                     /locus_tag="KRH54_001497"
     CDS             6392..7042
                     /gene="fsa"
                     /locus_tag="KRH54_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG8579164.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            7194..10118
                     /locus_tag="KRH54_001498"
     CDS             7194..10118
                     /locus_tag="KRH54_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8579165.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
     gene            10710..11168
                     /locus_tag="KRH54_001499"
     CDS             10710..11168
                     /locus_tag="KRH54_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8579166.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            11229..11504
                     /locus_tag="KRH54_001500"
     CDS             11229..11504
                     /locus_tag="KRH54_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8579167.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            11563..12837
                     /locus_tag="KRH54_001501"
     CDS             11563..12837
                     /locus_tag="KRH54_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBG8579168.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            12967..14019
                     /locus_tag="KRH54_001502"
     CDS             12967..14019
                     /locus_tag="KRH54_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG8579169.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            14019..15050
                     /locus_tag="KRH54_001503"
     CDS             14019..15050
                     /locus_tag="KRH54_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBG8579170.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            15142..15954
                     /locus_tag="KRH54_001504"
     CDS             15142..15954
                     /locus_tag="KRH54_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG8579171.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            15957..16877
                     /locus_tag="KRH54_001505"
     CDS             15957..16877
                     /locus_tag="KRH54_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG8579172.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            17052..17498
                     /gene="rpiB"
                     /locus_tag="KRH54_001506"
     CDS             17052..17498
                     /gene="rpiB"
                     /locus_tag="KRH54_001506"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG8579173.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            17528..18193
                     /locus_tag="KRH54_001507"
     CDS             17528..18193
                     /locus_tag="KRH54_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG8579174.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            18303..19805
                     /locus_tag="KRH54_001508"
     CDS             18303..19805
                     /locus_tag="KRH54_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBG8579175.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(20117..>21160)
                     /locus_tag="KRH54_001509"
     CDS             complement(20117..>21160)
                     /locus_tag="KRH54_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG8579176.1"
                     /translation="LSMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFFMYIYYAEAI
                     AIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLKVIGDDEISTL
                     AKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDMLKNNDFDRE
                     TINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQSIVEFEDKI
                     QNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYIDMFIKEENII
                     LTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELENAHMEIIIDA
                     DLFKVMLTFRRVN"
BASE COUNT         8554 a         2218 c         3932 g         6456 t
ORIGIN      
        1 aaattaaaca aaaactaaaa tgacaaatgt cattatctaa agtgacatag aaacggtttc
       61 ttaaagttgt tactataaaa tataaactct ataataaaag tataacaaaa gggaatcata
      121 tcgatataga atataattca agaggaggta aaattatgag tagttctaat caatcgacaa
      181 taaagacatc tatacagcga gtagggaaat ttttaagtgg tatggtaatg ccaaatatag
      241 gagcttttat agcatggggt cttataacag cattatttat accaacagga tggatgccta
      301 atgaaaaatt gagtactata ggtgacccaa tgataaaata ccttttacca ctattgatag
      361 cttatactgg tggtaaagca atagcaggac aaagaggtgg cgtaataggt gcagcagcag
      421 ctatgggagt aattgttggt gcagatatac caatgtttat cggagcaatg ataatgggac
      481 catttgcagg atgggtaatt aagaagtttg ataaatttgt agatggaaag ataccaacag
      541 gatttgaaat gttagtaaat aacttttcaa taggtataat aggtatgctt cttgcaatac
      601 taggatttta tgcaattgga ccagcaatag tagcaggaac agctcttata gaatctggag
      661 ttcaatttat agttagcaaa agtttattgc ctctagtttc agtatttata gaacctggaa
      721 aagtattatt cttaaacaat gcaataaatc atggtatatt agggcctata ggtatagctg
      781 aggcaaaaga agcaggtaaa tctataatgt tcttactaga aagtaaccct ggaccaggtt
      841 taggagttct attagcatat tggatgttta gtagaggaag tgtaaaacaa tcagcaccag
      901 gagctgtaat aatacatttc tttggtggta tccatgaaat ttacttccca tatattttaa
      961 tgaatccagt tttaatatta gctacaatgg caggtggagc agcaggaata ttgacttttt
     1021 caatattagg agctgggctt gtagcagcac catcaccagg aagtatattt gcacttatgg
     1081 cactagctcc aaagggagga ttacttccag tattagcagg agttgcagtg gcaacagttg
     1141 tatcattctt agtggcagca ccatttgtta agagagcttc agcaaatcaa agtgaagaag
     1201 acagtacttc tttagaagaa gctaaagcaa aaatgagtga tatgaaatct gcaagtaaga
     1261 actctgagaa aaatattgag gaaaaacagc ttgaagtaaa tgagattaag aagattgtat
     1321 ttgcatgtga tgcaggaatg ggttcgagtg caatgggagc ttccagattt aaaaatagaa
     1381 taaaaaactt agacttaaat atagaaatta caaattcatc tgttgataat cttccagatg
     1441 atacacaaat agtagtaact cacaatacat tagttgaaag agttgctaag aataattcaa
     1501 gtgttgagat agtttcaata aacaattttt taaatgaccc aaatttagat acattgttta
     1561 aaagattaga atctaaataa aaataaaaag taccactaat taggagtgga taatttgaaa
     1621 aaaaagaaga ttacatctag gcaaaaaaag ataatactaa tgatagtaga aaattcaaag
     1681 aaaaacattc caataactat ttctgagatt gcagggactt tagagttaag ttcaagaact
     1741 gtacttagag atatgtctgg aattgaaaaa tggtttgatg aaaatgattt taactttgta
     1801 aaaaaaccag gagtgggatt gattttagag gaaaatatag aaaatcaaaa ctttataatt
     1861 gagttgttag aagaagaaaa aatagaaaaa gaatacagca aggaagaaag aaatttaatt
     1921 atattatcaa agctactagt ttctaacgag ccagtaaaat cctattattt tgcaaaaata
     1981 ttaaaggtat ctgaaggggt actgaataat gattttgcct tagcaagtaa atggttggaa
     2041 agatttgata ttgagcttgt tagaaaacca gggcttggtg tctatttaaa aggtcaagaa
     2101 aaaagcttta gagaggcata tgtaaatctt atatacgatt cattcaatga aaaagaaata
     2161 ctagatatgg tcagaaatat aagtgaaaat atacaaacag ataaagcaat agaaatttta
     2221 agtgaaaata gacttttaaa tttaatggac aggtgcataa taagaaaggt tgaagctaca
     2281 ttaacaaaga agctttcaga tttagatgta aatttagctg atagtgcata tatagggctt
     2341 gtagttcata tttcattagc cttacagagg ataaaaaatg gtgaaaatat aactatggat
     2401 aaagagtttc taaaagaatt atctataaca gaagagttta aattagctaa agaaatcgta
     2461 aaaggtatgg aaacagattt taacatggat ataccagtag atgaggttgg atatataact
     2521 atgcatataa gaggtgcaaa gcaaaggtca tcttcaaacc ataaggcttt aaacttagat
     2581 gatatagaaa taatggaaat tacaaataag atgatagatt tggcggaaga tgaatttaaa
     2641 ataagtttaa aaaatgacga gaggttattt aaagacctag caaatcactt agggccttct
     2701 ataaatagat tgaatatggg attagaaata agaaatcctc ttttagatga aataaaaagc
     2761 aagtattcat atgcctatga tggagttgaa aagatttcta gaattataaa agataaacta
     2821 aatataaatt caatacctga atcagaaata ggatatattg caatgcactt tgcatcagcc
     2881 atagaaaaaa atcttatgat gaatactaat ataaatattg ttgtggcatg tcccactgga
     2941 ataggtactt caagattctt atcaactaag attgaaaata aatttcctaa tttaaatata
     3001 cttgaaacca tatcagctat aaacatagat gaagagtatt taaaggaaaa agatgttgat
     3061 ttaatagtgt ctacagttga gttaaataca agtttaaatt atatatgtgt aggtccattt
     3121 atgagtttag atgatgaaca gataatcaag gaaaaaataa aatctatagc tcaaaataaa
     3181 ttaatcaatt taaatgcgaa aaatgatact aagagtaaga ataaagtata tgaacagata
     3241 acagaaagta tgaatatagg aaaagatatt cttcaatttt tagaggaaat tagatttgaa
     3301 aaatttgaaa gtaaagattt aagtgagctt attgaagact catcaaaaat atttgcaaaa
     3361 tctagtgaag atataatctc tataaaagaa tcgttaaaag aaagattaaa aatttcaatt
     3421 ccatatatag aagaatcaaa aattctattg ttacattgta tgagtgaaag aatagatatt
     3481 atgaagttat caataataaa actagagaat agaatcgtgc tagattcaaa tgaagaaatc
     3541 gacaatgtag tttttatgtt acttcctaaa aattcaccaa gttatcaaag acaaataatg
     3601 agtgaaataa gtggttcatt aatagacaat ataattttta ctaataagat aaataaattc
     3661 tctatagaag agatggcatt agaggtaaaa gatatagttt ttaattttta tacaaacaga
     3721 ttaaaagcat ttatagataa ataaaattaa tattaaaaag gaaggttgat actatgagta
     3781 aagtattgaa tgagaataat atatttttag gattagatag tgtttcaaag gaagaagcta
     3841 taactttagc aggaagaaaa ttagttgaaa atggatatgt aaaagaagaa tatataccag
     3901 caatgttaga aagagaaaaa gtaatgacta catatatggg aatgggagtt gctattccac
     3961 atggtgtaaa tgaagcaaaa aaagaaatat tatcttctgg aatagttata ttacaattcc
     4021 ctaatggtat agattttgat ggagaaaaag catatttatt aataggaata gctggtgttg
     4081 gagatgaaca tttagaaata ctatctaata tagcagtagt tttagatgat gacttaacag
     4141 aaagactaaa aaattcaaat gacaaacaag cttttatgga agcttttgcc aactaatctt
     4201 aggagggatt taaaatgaaa aaggcaattc agtttggagc aggaaatatt ggaagaggtt
     4261 ttattggagg cttgttagtt aagtcgggtt atcatgtagt atttgctgat gtaaatgagg
     4321 ggatattaaa ctcaataaac aaagataaaa agtatactat acacataaga gatgtagaat
     4381 gtatagatga agtaattgat aatataagtg cggtttcatc tataaaagaa gaaataatag
     4441 atgagattgt acaggctgaa ataataacta cagcagttgg acctttagtt ttgactaaaa
     4501 tagcctctac aattgcaaaa ggtataaagg ctagaaaaga aaagggatta acaagtaatc
     4561 taaatataat agcatgtgaa aatgcaatat acgcaagttc ttctttaaaa gaagaggtac
     4621 ttaagtattt aaataaggaa gaagtagaat atttagagat gtatgttggt tttccaaact
     4681 gttcagttga tagaatagtt cctcctggta aaaacgaaaa tcctcttgat gtaacagttg
     4741 aaaattttta tgagtggaat gttgaaaaac aaggatttaa aggggaaata ccaactatag
     4801 ttggaatgaa tttagctgat aatcttatgg cttatataga acgtaaattg tttacactga
     4861 atacaggtca tgctataaca gcttatatag gatatttaaa aggatataag actatagaag
     4921 agagtataaa agataaattt atctgtgata tagtcaaaag tgcaatggta gaaagtggag
     4981 aaggattgat taaaaaatac aattttgact cagaagtgca ctataaatat attgataaaa
     5041 ttttaaatag atttaaaaat ccatatctta atgatgatgt attaagagtc ggaagggaac
     5101 cattaagaaa actaagtgat aaagatagat tgattaaacc tttaatgaca gcaaaatcat
     5161 atggattatc agttgataat ttgattcttg gaataggtgc agcgcttcat tataataata
     5221 gtgaagatac tcaaagtgta gaattacaag agttgataaa gtctattggg gtaaaaaaag
     5281 ccgttgctaa aatagctaat ataagcaatg acgaagaact tttaaataat atcgaaaaaa
     5341 gttatatatt tatgaaaaat ttataaattt acttgaaaaa aagtagttct gataaagtaa
     5401 ctataatata ttattctaca cagtttgaat tatttgttga ataatagaga attataatat
     5461 ataattaaaa ggaataaatt aatatattta cctgtatatg tttgataata tggttcaaat
     5521 gtttctacta agctaccatt aatagcttga ctacaggagt gaagaagata tagtttatac
     5581 ttattttaaa ttatatatta agttaattac tttgaatttt aagctcctgt ctatgatata
     5641 gacaggagct tttttagtat tttaatataa aatacttaca agaatagata gcaaaattag
     5701 aacaaatata atttttttat taatataatt ttagaaaggg ctaggtggaa gttcaatgaa
     5761 ggctttacaa gaaaagatat tgagagaagg tagtgtttca ggaaatgata tactaaaagt
     5821 tgatagtttt ttaaaccatc aaattgatgt agctttttta aatgaaatag gaagagaatt
     5881 taaagagaga tttaaaggag aaaaggtgga taagatattt acaatagagg cttcaggaat
     5941 agctatagca tcaattgttt cacagtattt tgataatgca cctgtagtat ttgcaaaaaa
     6001 atcagaatct aaaaatttgg ataaagatgt atatgaaact aatgtatatt catttactaa
     6061 ggcaagagag tattcagtta aagtttctaa aaagtacata aacaaaggcg aaaatatatt
     6121 gatagtggat gattttttgg caaatggaag agctgctctt ggacttaaag atttaattga
     6181 gcaagcagaa gctaacttag ttggagtagg aattgtcatt gaaaaaggat ttcaagctgg
     6241 aggagcactt ttaaaggcta atgatgtaag attagagtct ttggcagtag ttgaatctat
     6301 agataatgga actgtaaaat ttaggtaatt tatgatatta tatatttgtg tttagcacat
     6361 attttaacta tgtaattatg gaggattttt tatgagattt tttatagaca cagccaatat
     6421 tgaagaaata aaagaagcaa atgatttagg tgtaatctgt ggtgtaacta ctaatccgtc
     6481 tctaatagct aaagaaggta gagattttat agaggtagtt aaagaaataa gtgaaatagt
     6541 agatggacca ataagtgcag aagtaataag tttagaacat aaaggtatga tagaggaagc
     6601 agaaaaatta tctaagatac ataaaaatat agttataaag atacctatga cagcagaagg
     6661 tctaaaagcg gtgaaagtac tatcaagtaa aggtataaag actaatgtaa cgcttatatt
     6721 ctctgctgga caagctcttt tagctgctag agctggagct acatatgtaa gtccttttgt
     6781 tggtagattg gatgatatat cacaaaatgg tcttgattta attgaagaaa tcgttgatat
     6841 atttagtgta aatagtattg aggctcaaat aatagttgct agtgttagaa atccaataca
     6901 tgttttacaa gctgctagaa tgggagcaga tatagcaaca gttcctctta aagttataaa
     6961 ccaaatgatt aagcatcctt tgacagacaa aggaatagat agctttatga aggattggga
     7021 aggtgcttct ttaaaattat aattataaac tacgctttat aagtatatta aagaggttat
     7081 tatggaaatt ctaagtaata aggctaggct attttgccta gtcttatttt ggtgtataat
     7141 atattgaaac atattattga aatagattaa ttttttaagt gaggaggcga aaaatggata
     7201 aaatattaag agagatacaa agagaagata aaaaaaatcc atatactgac caagaacttg
     7261 ctgaaatgtt aaatgtagct aggtctgaag tgatatcagt taggaaaaaa aataatattt
     7321 tagattcgag agaaagaaga aaaaaaatat taataaaaga tatatcaaaa attgtaagtg
     7381 aaaatcctca aatgtctgaa agaaaaataa ctgaaatatt aatagaaaat ggatataata
     7441 tatcgagaag tgcagtttca aaattattaa aagaggaaaa tttaaatcag tcaaagattg
     7501 ggattaagga taaagaccca gcatcatcag gaaagtatgt gaaggtgagt aaagttgtag
     7561 aagaagatgg atttgaagaa ttgataggta taaagggaag tttgaaagaa aaggtaaatt
     7621 tagctaagtc agcaataatg tatcctccta atggccttca tactataata tatggagaaa
     7681 caggagtagg taaaagtgaa ttagctacat gtatgtataa atatgctata aaaaacaaca
     7741 ttaaagaaga gaacagccca tttatagtgt ttaactgtgc agactatgca gaaaatccta
     7801 atctattgat agctcaactc tttggggtag taaaaggtgc atatacagga gcagatgcaa
     7861 atagagaagg tcttgtagaa caagctaata atggaatatt gtttttagat gaaattcata
     7921 gattgcctgc tgctgggcaa gaaatcttat tttcattaat agatagagga gaatttagaa
     7981 ggttaggaga aagtagttct aatagaaagg caaatgttct cataataagt gctacaacag
     8041 aaaatccaga ttctaattta cttcaaacat ttagaagacg tataccaatg gtaataaatt
     8101 tacctaatat gagtgaaaga ccaaaatctg aaagatatga aataataata aagttttttg
     8161 aaagagaagc aaagcgagta aacaagaatt ttataataac taaagaagtt atgtgtgcac
     8221 ttatgaatta taagtgtact ggaaatatag gtcagttaaa gagtgatata caagtaactt
     8281 gtgctagagc ttttagtaaa tcgatatttt ctagtgataa agtaattata gatttagata
     8341 gtttaaggga ttatataaaa agtggatatt atgattgtaa ttgtaaaaat gaaaagtatt
     8401 ctgagttttc agttgaggat atatatatag atattagtga attggtgagt ggaaaaaata
     8461 gaattttatc tgataatgag ataagcgaga tatataatta tgcagaaaaa gagcttaagg
     8521 ttttggaaag taagtgcttt tcagaagaag agttaaaaaa tgcatttata gaaaagttag
     8581 ataagaagtt taatgctatt aaaaacaata aaaatttatc tgatagaaga agtcgaatag
     8641 actggggagg acaattaaaa gagtctacac tggagataat ggataaggta attgtattta
     8701 ttaaggagca gtttaagcat gtaaatcaag ggctgtactt ggctctagca attcatattg
     8761 agcatgctat aggtaggata aaagatggaa aaactataat aaatccgagt ttagataaaa
     8821 taaaagcatc aatgccaatg gaatatgagt tgtcaagata tataatgggt atagtagaag
     8881 atttgacaca agtaacattt ccagaagatg agcttggata tttggcttat tatataaata
     8941 aattttgtta taatgaggaa agtataaaag ataaagttaa agtagtaata gttacgcatg
     9001 gtaaagttgg aattgaaatg tctaaagttg taaatcatat tttaggtata gaatgtacac
     9061 taggtataga aatagcactt acagattcac catcagaggg gatagagcat gtattagaag
     9121 agcttcaaaa gatagaagct agaaaaggta ttctagttct aattgatatg ggttctttgg
     9181 taattcttgg tgatgaggtt gaaaaaaggc ttggaattag atgtaaaact gtaaatagag
     9241 tagatacatt attggcaatg gaagcaggaa aacttgcaac cattgaaggg aaaagtttag
     9301 atggtataat agctgattta aagaaaaata agaattatgc tatgataaat acaaataaat
     9361 ttagttacag aaaaaacgag tatggaaaga aaaatgtaat cattacttta tgtttgagtg
     9421 gtgtaggtac agctttaaat ttaaaagagc atatagaaaa acaaattaag gaatatgaca
     9481 ctttaattga agtaaagcca gttgcttttt taaataataa tttagaagaa gagttaaatg
     9541 atattgaagc tgaatataat attattgcta tctgtggaac tattgatata gattataaaa
     9601 acgtgccttt tatttcttat aatgaggtac ttggtaaaaa tggtattaat aaagtattag
     9661 agcatttaaa tagtaaacaa ccagctttaa atattgaaaa ccagttgaat aatttaatac
     9721 atgaagattt gattttatac aattttgaag gaatttcaaa ggagtatata attgatactc
     9781 ttgtttcaaa acttgaagaa ggtggatatg tcgactcaaa gtatatatta agtgtatata
     9841 aaagagaagc aatgggaagt gttgtaatgg cctctaaggt agccgtgcca catggacttc
     9901 cagaaaacgt tataaagcct gcaatagcaa ttgcgaggtt gaataaacct atagtatggg
     9961 acaataaatt tatggtggat ttggtggtac tactggcact taaagaaaat aataagaaag
    10021 aaatacgaag cttattttca aagattaatg accagcatac tttagaagtt ttattaaata
    10081 ctgaagataa aaatgaaata aaagaacttt taagctaatt gttagtaccg agaagaaaga
    10141 caaaaaaatt tgctttagta gtataaaatt taaaactact caattgctaa gtagttaatt
    10201 actctatata aggctagttc tatgaaattt atttatatga ttttatagaa ttagcttttt
    10261 tcttggtaaa taaaatatat gaatttattt atgtaaaaag taaattttat tggacaattc
    10321 tataaaaatt tttcctatat ttcttataaa atatagaata tataaaaaat atataaaaat
    10381 aaattaaatt cataataaat aattttataa aatagttgtt aaacataaaa aaccatttgt
    10441 aaaaaacaaa aaaatcattt ctctaacaaa aaaaaccaca aaaaatataa aaaagtatgg
    10501 ctagttaagt gattaacaag taaaaattaa aataaaaatc aaaaaaaatt tatcaatgaa
    10561 agtcagctaa aatcctggaa gagagcatta tttttgggaa aacattttct tttgtttcac
    10621 aaaaaaagcc actttttggc acacaacatg ctttatataa agtcaagaaa aactaaggta
    10681 taagacaaag gcaattagga ggtgtaaata tggagtttat aaattttgta agtgataaag
    10741 ttatagacct taaaatggat tgtaaaacga aagaagattt ctttaaagaa atacataata
    10801 aagtttttga tttaggcttt gtaaaagaag aatttggaga aaaaatatta gcaagagaaa
    10861 atgtatttcc aacaggttta aatttaggtg attatggagt agcaatacca catacagatg
    10921 cggaatatat taaagagcaa tttatatcag tctgtacttt taatgaacca gtagtattta
    10981 gttctatgga agaccaagat gaaaaagtac ctgtaaattt ggctttcgta ttaggactta
    11041 atcaaccaca cagtcaatta tctgtattaa cagaattaat gggaattatg caaaataaag
    11101 agttagttga taagttgatg agttctactg ataaagaaga agttttaaag acactaaagt
    11161 ctttataaaa ataaaaaagt actaaaaata aaaaatataa aaataaaaat tattaggagg
    11221 aaataaaaat gaaaaaaata ttagtagcat gtggagcagg aatagcaaca tcaacagtag
    11281 tttgtgacaa ggtagagaga ttagttaagg aaaataatat acaagcagaa gttatacaat
    11341 gcaaaatagc tgaatgtgca acaaagcaag aaggagcaga tttaatagta tcaacaacta
    11401 tactaccaac aacttataat ataccagcaa taaaagcaac agcatatata actggagtaa
    11461 atacagcagc attagataaa aaaatattag atgctttaaa ataatcctat catagataca
    11521 ttgaaataat attttataaa acagaaaaag gaggatttat aaatggataa attattagca
    11581 gtagtacaat acattttaaa tatgggaccg acagtaatac taccaataac aattgtagta
    11641 ctaggaatga tatttagaat taagtttaca aaagctttaa agtcaggtct tacaattggt
    11701 ataggatttg tgggtataaa cttagttgta aacctattaa catcaacact agggccagcg
    11761 gctcaaggaa tggttgaaag attcggactt aatttaacag taatagatgt tggttggcca
    11821 acaacagcat cagcaacatg ggcttcacca gttgcaccaa tattaatacc agtttgttta
    11881 ttagtaaact tactattatt atacttcaat aaaacaaaga tattaaacgt tgatatatgg
    11941 aattattggc actttatagt agcaggtgca gttggatata tagtaacagg aagttatgtt
    12001 tgggctatat tctgtgcaat actaatggag ttattagtac tgtttattgc agaaaagaca
    12061 gcaaaaaatg ttcaggagtt ttatgattta gaaggtatag cattaccaac tggttcaaca
    12121 gcttcatttg ctccacttgg atatttagta ggtaaatttg ttgaaaaaat accaggaata
    12181 aataaaatac aagcagaccc agactctata caaaaaagat ttggtatatt tggagaacca
    12241 atgatgatgg ggattatact aggtataata cttggagcac ttgcgggata tgatgtagca
    12301 ggaatattca atgtaggtat atcaatgggt gcagtaatgt tacttatgcc tagaatggtt
    12361 agaatattaa tggaaggttt aattccaatt tcagaatcaa taagagattt cttacaagca
    12421 aaatatggag acagagaact ttatataggt ctggatgcag cagtagcaac tggacatcca
    12481 gcagttatat caacagctct tatacttgtt cctataactt tgttcttagc agttatacta
    12541 ccaggaaaca aagttttacc atttggagac ttagcgacaa taccatttta tatggcattt
    12601 atagtgtgtt ttagaaaagg taatatagtt caatcagtta taacaggaac aatagtaata
    12661 gctatatcac tttatatggc tacaaacttt gcagatgttc atacacaatt attacaacaa
    12721 gcaaatatga tgccagaagg aacaacaatg gttacaagta tagatacagg tggaaacttg
    12781 ctaaaatggg gaatattgaa attatctcaa ttagtttctt catttggaat attctagtat
    12841 gtagttaata gagattactc ttttttagta aagtatgcga aaatttaaca tttagcagct
    12901 actaaaaaag agtaatattt tttataaatt tttaaaaata caaaatacaa gaggaggaga
    12961 tttatcgtga aagcagcagt attacatggc acaaatgata tgagatttga agatatagaa
    13021 ataaaaccat gtgaaagtga tgaagtaaaa ataaaagtta tggcagcagg aatatgtggg
    13081 tcagatcctc caagagtatt aaaacattgg aaatatccag ttccagctat accaggacat
    13141 gagttttctg gagtaattgc tgaagttggt aaggatgtaa aaaatgtaaa agttggagat
    13201 agagtagttg caattccttt tataccttgt aatgaatgtg aatattgtaa aagaggatta
    13261 ttttcattgt gtgatgacca tggaatgtta ggagctaaaa gttttggagc ttttgcagag
    13321 tatgtaaata taaaagctac aaatgtactt ccaataggag atatggactt tgaagatgct
    13381 gcaatgatag agccactagc agttgctatg catggagtac ttaatatagg tgttcaagtt
    13441 ggagacactg tagccgtaat gggtagtgga acaatggggc agttggtaat acaaggactt
    13501 aaaatagcag gggcaggaac aattatagca gttgatatat ctgataataa attaagagaa
    13561 tcaaaggaat taggggctga tataataatt aatgctaaag atattaaccc agtagaaaaa
    13621 ataaaagaac ttactggtgg aaaaggtgtt gacatagctc ttgaatgtgc aggttcaaaa
    13681 ataactcaag aacaatgttt acttataact aaaaagaaaa gtaagatagg atttttaggg
    13741 atagcatatt cagatataac tttatcagaa gaagcatttg aaaatatatt tagaaaagaa
    13801 ctagaattaa aaggtttttg gaactcttat tctgcaccat ttccaggaca agaatggact
    13861 aagggaatca atctagttaa tgaaggaaag ataaaattaa aagaaatggt atctcataga
    13921 ttctcacttg aagatactta caaagcattt gaaatgataa gagatagaaa agaagaattt
    13981 aacaaaatat taattttacc acaaggggtt gaaaaataat gaaagcagta gttaaaacta
    14041 aaccaggcta tgataatgta gaagttttag aagtagaaga accgaaagct acaggagaca
    14101 aggttaaaat aaaagtagaa tatagtggta tatgtggttc agatatacat tcatttaaag
    14161 gtgaatatgc aaatataaaa gccccagtaa cattaggtca tgaattttct ggaattgttg
    14221 tagaagtagg agaagatgtt aaaaatataa aagtaggaga tagagttaca tcagagacta
    14281 cttttgcaac ttgtgagaaa tgtatatatt gtgaaactaa agattataac ttatgcccaa
    14341 ctagaaaagg tattggtact caagtaaatg gaagttttgc tgagtatgta ttatcaagag
    14401 aagaaagcat acatgtactt cctgaaaatg tatctttatt atcagcagcc cttacagagc
    14461 cactagcttg ttgtgttcat gcagctcttg aaaagactac tatagaaagt tctgatacag
    14521 ttttaataat aggaccagga ccaattggtt tattattagc tcaagttgtt aaatctcaag
    14581 gtgctacagt tataatgtct ggtgttacaa aagataagga aagattagaa atagctaaaa
    14641 atcttggagt agatagaact gtaaatgtaa tggaagaaag tttggcagat gtagtgaatg
    14701 aaatgactga tggaaatgga gtaaacaaag gatttgattg ttctgggttt atgccagcag
    14761 ttaacgaagc cttaagactg cttagaaaaa aaggtacttt cgtacaagtt ggaatctttg
    14821 ctaaaaaatt aaatgaaatg gaccaagaag caataatcca aagagagtta caatatatag
    14881 gaagtagatc tcaaaagcca agttcttgga ttacagcatt agatttaatg gcaagtggga
    14941 aagtaaatac agaagcttta gttactagaa ctgttggttt agacgaattt aaagaaggta
    15001 ttacagcttt aatgaacggg gaagaaataa aagtagcagt aaaatcttaa aaaaacattt
    15061 gtattatttt aaaaaaaatt gtaaaatcta ctgtaggata aaaaaatgta aggtcttaaa
    15121 aaataacagg aggaagaaag tatgagcact cttagagatc attctaataa tattagaaaa
    15181 aacataataa aaatggttgc agaagctaaa agcggacatc caggtggttc attatcaata
    15241 gctgatatgt tgacagtatt atattttgat aagatgaacg tatgtgcaga aaatccaaaa
    15301 atggctgata gagatagatt tgttctatca aaaggtcatg cagcaccagc tttatatgct
    15361 acattagcag aaaaaggatt tttcccagaa gaagaattaa taacactaag aaaatttggt
    15421 tctaagttac aaggtcaccc agacatgaaa aaagtagcag gaatcgatat gtctactggt
    15481 tctcttggtc aagggctatc tgcagcaaat ggtatggcac ttgcaggaaa attagataac
    15541 aaagactata cagtatatac tatattaggt gatggagaaa tccaagaagg tcaaatatgg
    15601 gaagctgcaa tgacagcatc acattacaaa ttagataatt taattgcatt tgttgactta
    15661 aatggattac aaatagatgg ttctaatgaa gaagtaatga atgtaaatcc agtagatgac
    15721 aaattcaaat cttttggatg gaatgtaata gtaataaatg gacattcttt tgaagaaata
    15781 ggaaatgcag ttgatgaagc taaaaaagtt acaggaaaac caactgtaat aatagctaaa
    15841 acagtaaaag gtaaaggtgt ttcatttatg gaaaataatg ctgcatggca tggaacagct
    15901 ccaaatgcag aacaagcaga gcaagcattg aaagatttag aaggaggtct ataggaatgg
    15961 gaatagcaac tagagaagct tacggacaag tgttaaaaga acttgcagaa aataaagata
    16021 tagtagtatt agatgcagat ttaggaaaag ctacaaaaag tatatcattt aaagaagttg
    16081 ccccagatag attttttgac atggggatag cagaaggtga catgattggt acagcagcag
    16141 gtcttgctac ttgtggaaaa ataccttttg cgagtacttt tgcaatattt gcagcaggaa
    16201 gaggctatga gcaaataaga aactctgtag catatccaaa tttaaatgta aaaatagcag
    16261 ctactcatgc aggtgtaaca gtaggtgaag atggtggtag ccatcaagct atagaggata
    16321 tatctctaat gagaggtata ccaaatatgg ttgtattaaa tccagcagat gctttagaag
    16381 caagacaagc tattcttgca tctattgatt ataatggacc tgtttatata agattaggta
    16441 gagctgcaac tcctgatgta aacagtgaaa attataaatt tgaaatagga aaaggaactg
    16501 ttttaagaga aggttctgac ataacagtaa tagctacagg aataatggta gcaaaagcct
    16561 tagaagcagc agaagaactt gctaaagaag gtataaatgt agaagttgtt aatatatcta
    16621 caataaaacc attagatgaa gcactaataa aagaaactgc taagaaaact ggaaaagtag
    16681 ttacagcaga ggagcacagt ataataggtg gtttaggttc agctgtatgt gaagctttag
    16741 cagaaactaa agatgtagta gttagaagaa ttggtgttaa agatgtattt ggtcaatcag
    16801 gtacaccagc tgatttatta aaacactatg gtttaactac agaagatata gtaaaaaata
    16861 taaaagagtt gttatagata taaatatatc taaataacaa attgatttga cataagtcaa
    16921 ttaaagctgt cttaaaaata gaaagaaatt tctacaatgg gacagcttta tttttacaat
    16981 actttatttt tatgataatt ataaaatagt ctgaaagtaa tcaaaaaaat tataaaaaga
    17041 ggtgctattt aatgaaaata tctattggat gtgaccatgg tggttatgaa ttaaaggaat
    17101 atataaagga aaatttaata aataaaggta tagaagttga agatgtgggt acaaattcaa
    17161 aagaaagtgt agattttcca atatatgcaa aaaaggtaag tcaaaatgtt caaagtaaaa
    17221 aatctgattt aggcatactt tgttgtggaa caggaatagg tatgtctatt gcagcaaata
    17281 agtttaaagg aatcagagca gctgttgttt ctgactgttt ttcagctaag gcaactagag
    17341 aacacaataa tacaaatgta ctttgtttag gggaaagagt tattgggaaa ggattagcaa
    17401 tgagaattgt tgaagaatgg ttaaattcaa gttataatgg agaaagacat gcaagaagat
    17461 tacaaatgat tgatgatata gaaaaagaaa atttataaat atataattta taacaagggg
    17521 gagcgttatg tctattattt gtgcttcaat aatgtgtgca gaccaaatga agttaaaaga
    17581 ggaattagaa gctcttgaag aagcaggtgt aaaattactt cattgtgatg ttatggatgg
    17641 aatatttgtt aaaaacttgg ctatgggacc agagttatta aaatcaataa gtgaaaatac
    17701 aacaatacct ttagacatac acttagccac agaaactcca gataaatata ttgatatgat
    17761 gtcttatata aagccaaaat atatttcttt tcatgtagaa tcaagtacaa atgtaaaagc
    17821 tgatatacaa aagttaagaa attatggtat agggccagtg ttagcaataa gccctcaaac
    17881 aagtgtggat aaaatagaag aatatataag tttagttgag ggtatcttag taatgacagt
    17941 aaatccaggg tttgcaggtc aaaagtttaa tctttctgta ttggataagt tggataaact
    18001 tacagagata ctaaaagatt atgataatcc tccatttata gaggttgatg ggaatataaa
    18061 taaggatact attaaactta tgaatggtaa aaaagtagat atatatgttg ttggtacttc
    18121 agctttattt aatgataagc ctccaatttc atataaagat aaaatagaag aactaaaaga
    18181 aagtataaag taaccatgta ataaaattat aatatgtttc tgtttaatag taaaaattta
    18241 ggtggaaaca agaaatttta taatatttaa taaaaatttt ggttgagtaa ggagaatttt
    18301 ttatgggaga cctatataaa ctacaaaatg gtactgatat tagaggaata gcgtatgaaa
    18361 atgattctaa agaagttaac ctaactgtag aagaagtaaa aaaaatagct aaagcgtttc
    18421 atatttggtt aaaagaaaag actaagaaag ataaagtaac tgttgccata ggaactgatt
    18481 ctaggataac aggaagccaa tttagaagta ctgttattga aactttaact aatgacgatt
    18541 gtaatgtaat tgattgtgag attgccacaa ctccagctat gtttatgaca actattatgg
    18601 atggatacaa ttctgatggg gcgattatga ttacagcaag tcacttacct tattactata
    18661 atggtttaaa gtttttcaca gaaagtggag gtttagaaaa aactgatata aaagagatgt
    18721 tggatatagc tgttaaaaat gatagtgtag actatgagga agtaaataaa aaaggtgaag
    18781 ttatagtaaa aaatttaata gaagattatt ctaatttatt gatagataaa attagaaaag
    18841 gtgtaaattc aagtaaaaat tatgaaaagc ctttttctgg gcttaagata ttggttgatg
    18901 caggaaatgg ggctggtgga ttctttgctg aaaaagtttt acacatactg ggagcagata
    18961 cgacaggtag tcagtttttg aacccagatg gtatgttccc aaatcatata cctaatcccg
    19021 aaaacaaaga agcaatggaa agcatatgta aagctgtgtt ggataataag agcgatctag
    19081 gaataatctt tgatacagat gtagacagag ctgctattgt aggtaaaaat ggcaaaccaa
    19141 taaataaaaa tgctcttata gctgttatat catcaatagt attagaagaa catccaaaaa
    19201 cagctatagt aacagattca attacttctg aaggacttgc taaatttata aatgaattac
    19261 aaggaagaca tcatagattt aaaagaggat ataaaaatgt aataaatgaa gctataagat
    19321 tgaatagtga aggtgaagag tgccatttag ctatagaaac gtctggacat gctgctttaa
    19381 aagaaaatta tttcttagat gatggggcat atttaatagc taaaattttg ataaaagtag
    19441 caaagctatc cttggaagga aagactatag aagaattgat agaaaacctt gaagaagctc
    19501 aagaagagaa agaagttaga ataggcatta ataaagaaga ttttagacca tatgcagagg
    19561 atatattaaa agatttagat tctcatgtta agagtattga tggatggtct gtagcaccaa
    19621 ataattttga aggcataaga gttaattgtg atgaatcaaa tggagatgga tggtttttac
    19681 ttagaatatc actacatgaa ccattattag ctttaaatat tgaatcaaat aaaattggtg
    19741 gagcagaaca aatttattct aagttaaaac ttttcttaga aaaatatgat ttaaaaggtc
    19801 tttagggtaa atagaaagaa tatatttata ttgaagaata aatgtaaatt tgtacctaag
    19861 attacaaata ttacaaaatt gtaatcttag gtaacagaga tgtaactgat tatggaaaca
    19921 ctatgtgata ttatagatac agagagatga aatatatgat gaattataga gaaatataat
    19981 ttaaaatgta ttaatatcac accaaaaagg tataatcaaa aaatatgtaa atataaaccc
    20041 caacatttag aaaaagacct ctaaggaggt ttttttcttg tttaaataac ttaaaatata
    20101 tttttatatt aatatatcaa ttaactcttc taaaagtaag cattacctta aataaatcag
    20161 catctataat tatttccatg tgtgcatttt ctaattcaat tatattttga gcaattgata
    20221 aaccaagacc agagccatct gtttttctag atatatcacc ccttctaaat ctttctatta
    20281 actcttctgg ctttagattt agtttatcat ttgaaatatt tttaaacgtt aatattatat
    20341 tttcttcctt tataaacata tctatgtaga ccctagtgtt aggcatagag tattttagta
    20401 tattgcaaat tagattctca aaaactctcc aagttttttt accatctatc atggtaaaaa
    20461 cagcatattc aggtgtttct attataaaat ctaaactgct attttgaatt ttgtcttcaa
    20521 actctacaat actttgaatt aaaagttctc ttatatcggt ttttgttata ttaaattcta
    20581 catctccact acttagctta gatgcttcaa atatgtcatc tattaataat tttaatcttt
    20641 gtgatttttt atctaaaata tttatatagt catttatagt ctctctgtca aagtcattat
    20701 tttttaacat atcaacatag cttaatatag atgtaagtgg agtttttaag tcatgagata
    20761 cgtttgtgat aagctctgcc ttcatccttt cattttttat attagcatct acagcagttt
    20821 caagaccgcc tgttatatta tttatacttt tggctaaagt agatatttca tcatctccta
    20881 taacttttaa tttatacttt aaattgccat tttctatatt ttttatattt tttgatatgt
    20941 tatttatgta attaaactta acagataaaa agttgtatat ataaaaatga aaaactgtgt
    21001 ttacaagtgt aaataaaatt actatagcta ttgcctcagc ataatatata tacataaaga
    21061 atatcaaaac aagatattgg ataatagctt gtacaactat agatattaaa aatactatag
    21121 ctaatttttt atttatagtt tttttattat acatactaag
//
LOCUS       DAEOSP010000050        20904 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.050, whole genome shotgun sequence.
ACCESSION   DAEOSP010000050 DAEOSP010000000
VERSION     DAEOSP010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20904
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.050"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(150..332)
                     /locus_tag="KRH54_001510"
     CDS             complement(150..332)
                     /locus_tag="KRH54_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579177.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            645..1064
                     /locus_tag="KRH54_001511"
     CDS             645..1064
                     /locus_tag="KRH54_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HBG8579178.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            1195..3036
                     /locus_tag="KRH54_001512"
     CDS             1195..3036
                     /locus_tag="KRH54_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG8579179.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            3063..4949
                     /locus_tag="KRH54_001513"
     CDS             3063..4949
                     /locus_tag="KRH54_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG8579180.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLT
                     LSAISIRKYDKVNNFYFSAKKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSNNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELKKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYG
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNETRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            4957..6957
                     /locus_tag="KRH54_001514"
     CDS             4957..6957
                     /locus_tag="KRH54_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8579181.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEIVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            7219..8478
                     /locus_tag="KRH54_001515"
     CDS             7219..8478
                     /locus_tag="KRH54_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG8579182.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            8539..10326
                     /gene="iorA"
                     /locus_tag="KRH54_001516"
     CDS             8539..10326
                     /gene="iorA"
                     /locus_tag="KRH54_001516"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBG8579183.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            10328..10903
                     /locus_tag="KRH54_001517"
     CDS             10328..10903
                     /locus_tag="KRH54_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBG8579184.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            10956..12026
                     /gene="buk"
                     /locus_tag="KRH54_001518"
     CDS             10956..12026
                     /gene="buk"
                     /locus_tag="KRH54_001518"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG8579185.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            12244..12945
                     /locus_tag="KRH54_001519"
     CDS             12244..12945
                     /locus_tag="KRH54_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579186.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            13005..13511
                     /locus_tag="KRH54_001520"
     CDS             13005..13511
                     /locus_tag="KRH54_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBG8579187.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            13686..14708
                     /locus_tag="KRH54_001521"
     CDS             13686..14708
                     /locus_tag="KRH54_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG8579188.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(14855..15163)
                     /locus_tag="KRH54_001522"
     CDS             complement(14855..15163)
                     /locus_tag="KRH54_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG8579189.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(15223..15651)
                     /locus_tag="KRH54_001523"
     CDS             complement(15223..15651)
                     /locus_tag="KRH54_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579190.1"
                     /translation="MNNKLHELEKNLPEISCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(15874..17331)
                     /locus_tag="KRH54_001524"
     CDS             complement(15874..17331)
                     /locus_tag="KRH54_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBG8579191.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            17749..18663
                     /gene="nadA"
                     /locus_tag="KRH54_001525"
     CDS             17749..18663
                     /gene="nadA"
                     /locus_tag="KRH54_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBG8579192.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            18676..19977
                     /locus_tag="KRH54_001526"
     CDS             18676..19977
                     /locus_tag="KRH54_001526"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBG8579193.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            19958..20794
                     /gene="nadC"
                     /locus_tag="KRH54_001527"
     CDS             19958..20794
                     /gene="nadC"
                     /locus_tag="KRH54_001527"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBG8579194.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
BASE COUNT         8280 a         2124 c         3662 g         6838 t
ORIGIN      
        1 acttatttaa gtatacattt tgaaaaaatt aaaaaagcta tatatagatt tttaaagaaa
       61 tctatatata gcttttaagt ttatgtcaat cttttttgtt aatgaataaa taatatagaa
      121 tacaatatac aaaaaagttt cttgaaatgc taagataatc cccatttaca aagcaacatc
      181 attatactgt gtaaatttga atcaacaatg ttttcaccat atttcatcat aaaatcctct
      241 aattgtgtgt caataaatga ataatatcca cttatactat cgatgtatgc agtcattaag
      301 tgtatacatt catcactttt atctttatac atatatatct ccccctatat acaatatata
      361 aatttagata ccaaatcgta tcatattaaa tagttaagtg tgtaacatct atgcagttat
      421 atatttatat cattatatta ataactaaag tgagttttga caatagattt tgggaaactg
      481 gatgttcaaa gtgcaaactt ggattttaaa atcattaatt aagatagcgc ataggaatat
      541 atgtatacat aacttaatta taaagattat aaacaattac ttttattaac tttatttaaa
      601 tggtagaata acttggagat taataattat aaggaggata taatatgagt ttatataagt
      661 ttatatatga tgataaggaa tatttattaa aagaggagaa ctgtagtgct ttaataaatg
      721 atgaagaaaa tccagttcaa ggagtaagta tttcaaaaat aattgatata cttaatgaag
      781 cagaagaagt tgattttgat gtagaatatt atcaagaagc atgtccatta tgtttagaag
      841 gagtaaaaga aaagaaaaaa ttctttcctt ttttagaata tcacttttat attttcagta
      901 aagatggaga atatgtaata agcaatatat ctgttgatta taaaggacta tcttttaata
      961 aactatcaag ggcaaataaa gttgataata gttatattgt aagtgtgata atttgcgaaa
     1021 attgtcagga ttatatagtt cagatagaaa attgtatagt ttagtgtaaa gtatgtctta
     1081 atttaagaga aatttctaaa ttgagacatc aacttttttc aaaaatagta tggaaaatta
     1141 ctaaatttat gagataatgt aagtcggagg tgagttacaa tatatagtaa atatttggaa
     1201 gagatagatg atacaatttt agattcaatg tttaaactaa ccttgaatga aagattattg
     1261 actaataacg attttattga aaataaaatt gatgtagtaa agaatttaat tgattgctat
     1321 aaaactgaac aaaatagttt aatgttatta aaatcaaatt ttttgctagc tttattatat
     1381 ggtattcaag ggtttagtga aaaaatgaaa gagtatattt tgatttcctg taaatatatt
     1441 gataaatgct tacccaaaga ttataaattt ttagcacgat tttattcaca aatagctatt
     1501 ttaagtttaa aaaatgaaga tgtaaataag gcaaatttat atatggataa atttaatctt
     1561 atttgtgatg aaaataattt tctaattgaa gaaattattt ttaaatcaca acttatttac
     1621 attaaggcaa gtaagtgtat agaatcttct gtaataatta aagagataga aacgttgtat
     1681 ataaagataa aagaaattaa cgattttaat tgtaaaaaaa tatatttttt tattattggt
     1741 aaaatatatt ttttgttttt ggatgatgct ctaattgcta aggtaaattt tttaaaagca
     1801 agaaaatatg cagatttact aaaggatatg caagtatctt cactatgtaa tataaagata
     1861 ggtgagtgtg aatgttcgtt tgaaaattat gaatgttcca tggaatattt caatgaagta
     1921 ataagcaata aaaagtatag aaatgtaaat ataattcaaa aatatagagc atcaaacaac
     1981 ataactaaaa tattaataaa gactagaaat tatcctgtcg caataaataa tttagcaaaa
     2041 tctgaagtat atctagaaaa aattaaaaat catagcttaa aagaagtaga gaagtttgat
     2101 ttatttataa gtttagccat gtattatgca aaaagtaatg aaaaatcttt tgaacaatca
     2161 atatgctatt tagatagagc taaaaacata ttaaatatga taaaaaatat agaagaatat
     2221 ataatatatg atttagaaat aatttatcac caggcaaaga tatattatat ctttggatat
     2281 tatgataatg ccttaaacac aaataaaaaa ctccttgaaa aatctaaaaa tgctaaaaat
     2341 tataattatg tgaagttagc atacaagagt atatatttgt gttttgaaaa gattcaaaat
     2401 tatgaaatga gtatgaaata ctttaaaatg tattatgagt taaaaatgat gtatataaaa
     2461 gctcgaaata gaaattatat agattcctta aattatagac atgagtatat agaaaaagaa
     2521 ataaataaca tgagaaaagt aaaacttgat ttagataaaa aaaagtatgt tgaccatttg
     2581 acaagtgcat ataatagaac ttttttgaac gattttttag aaaaacaaga agttagtgaa
     2641 tattgcacag catttatgat agatgtagat tactttaaag aatacaatga tacttatgga
     2701 cactataatg gagatattgc tcttaaaaat ataactataa taataaaaaa atatctaaaa
     2761 aaagacatgc ttatgataag gtatggtgga gaagaatttc tgatattatc tgtatgtaaa
     2821 gattataaaa aaagtaaaat atttggaaga aaattatgta ggattgtaaa gaaattttta
     2881 aacaataatt tgacagttag tattggcatt gatacatgta aaaatagtag tatagatata
     2941 aaagaaataa ttgaaaatgc tgataaagct ttatataaag caaaggaaag tggtagaaat
     3001 agatgtttgc attatcatga ttttaagaat ttttaactgt tgaaatagga ttggtgattt
     3061 acttgaaaag gagtttaata gaagatttaa agagaaatat aaacaagata gaatacgata
     3121 tgttgactaa attctatcta aatttgacag atttaaacta catcttggat tatcacaaag
     3181 accttttaaa tacaaaatta gaagataata gtagagtcta tactttatat ttaattagtc
     3241 ttataaacta tataaaaaaa gatagagaag catcatacaa ctatttgata gagtcattaa
     3301 agtatattaa aaaattgtca ttaaaagatg attcagatat aatatctaaa atttatattt
     3361 atcttacttt atcagctatt tctataagaa aatatgataa ggtaaataat ttttatttct
     3421 cagcaaaaaa aattataaga caaaatcatt taaatgagtt gttggtattg ttaaatatga
     3481 gtttatgtgt agaaataagt gctttatatc agaccaaaac ggatgtaata gctttagtag
     3541 aagaagtgtc tttttttaaa actttaaaaa ataaagctct tgtaagtagg gcaaattata
     3601 tatttgggag agtttatcta tatttattta ataactttat aaaaagtatg gaatacttaa
     3661 ttgaatgtct aaaattggct caagaaaata atttatactc tatagaggct ttttgtaaat
     3721 gtataataag tttgtgttat ttagagagtg gaaataatgt agaagctata aaatacataa
     3781 ataatgtatt agatgttgca cacagtaata actcaatatc agtaactgaa agagtgtcta
     3841 taaaaataga ccttatatgg gcatatttaa aagaagatat gaataaagaa gcagaagtta
     3901 ttttattaaa gactataaaa ttaatgaaag ttgttgaagg tgtatataag gattatttat
     3961 attcatttat atttttatat tcagctgaaa ttgagttaaa gaaagataat tgtagttttg
     4021 aaaaggtaaa cacctattta attttatcaa aaaatatata taaaagtttt ggagatgttt
     4081 ttatatataa ttctaaacta gattacatat ataagttgta tggagattta tatataaaat
     4141 ttggtaatat agatgaaggt ataagttgtt atataaaagg tttaaattta gttaaaaaga
     4201 gttcattgaa tcttagaaaa atatctatat tttatgggct aatagcaaat ggatatgagc
     4261 taaaaaaaga ttttaaactg gcaatagaat attataaaag tatggattgg tattctgata
     4321 ggtgggaaaa aaataattcc tataaaacat ctcaagcaat tcacaaacaa tatgaactca
     4381 gacaaaaaca agagagttta agattactag tggatgataa taagaaatta gaaaatgata
     4441 ttttaaaaga tggacttact aaactatata atagaagata tctggaacag cagttggatt
     4501 tatttaatga aaccagagac aaaaatatgg tcgcaatact ggtcgatata gattgtttta
     4561 aaagttataa tgataattat ggtcatctag caggagataa agttatagtt aaggtaactg
     4621 atattataag aagtgtattt atagatatta ccgaacacat aatcagatat gggggagaag
     4681 aaattttaat attagtagat tctatagaat caatcggtgt tgaagagatt gataatatag
     4741 aattatatat taaaagagtt ttcaaacttc ttgaatttga gggtattgaa catatatatt
     4801 ctaaaataaa aaactatata actataagtg ctggagtaag tataaaaaga tgtcgtagta
     4861 gaaaagatgt tgaaatatta attgaagatg cagataagaa cctatataaa tcaaaaaaag
     4921 caggtagaaa tcaatacatt ataatttaaa attgaggtga ttttgttgcg aaaaaaaata
     4981 ggaattatag cctctgatat tgaattaaaa gaaagaatag aagaattata tagagaagat
     5041 gtagaaaatg gaactataat aatcgacata cttaacctag acttaatgga aaatcaaggt
     5101 cgtatacttg ttgaaaaagg tgcccaagct ataattggta gaggtggagg ttatagttta
     5161 gtaatagata cagtaaatgt acctgttata ccaatgaata tgaagtcaac tgacctttta
     5221 agagcaatag aaatagcaaa gaaatattct aaaaaagtag tattgattct tggagacaat
     5281 gaagtttctt ttgactatgt tggctggaga aatgtcataa gtactgaaat aactgaagaa
     5341 tggtttgaat ctaagtatga gataagaagt aaagttgtaa aatatataga ccaaaaagat
     5401 gaaattgtaa tcgtaggagg aggtttggca tgttcttttg caaggcaata tggaattgat
     5461 agtgtatttg caactgctag tgatgaatct ataagagaag ctgtagaata ttgtaaaaaa
     5521 ttattagata cattaggtga agaaaagttt aataatgaag tgttacgaaa tatacttgat
     5581 ggtataaaag atggagtaat tgctatagat agtaatggca gtataatttt gtacaatgaa
     5641 agtgcaaaaa acatgcttaa agtagagaga aaatgtgcac ttaataaata catcttagat
     5701 gtatttccta aaatggagtg gatgttagat tgtttacatg aaaaagagga cgtagaagat
     5761 agaaagataa gaaatataaa taatctaatt gttaatacta ggacaacatt gataaaagtt
     5821 gataatagta cttatggagt tttaggaata atacaagata taaccaagct acaaaactta
     5881 gaaagaaaaa taagatttga cttgaatcaa aaaggtctct atgctagata tacttttgat
     5941 gattttttat ttaaagataa attaactaaa gaatttattg aagaagctaa aaaaattgga
     6001 aaaagtgatt atacaacttt actttatggg gaaagtgggt ctggtaaaga aattatagct
     6061 catagtatac acaatataag taaaagaaaa gataggcctt ttgttgctat aaattgtgct
     6121 actatagcag aaaatttact tgaaagtgaa ctttttggct atgaagaagg tgcatttact
     6181 ggtgctagaa agggtggaaa aagagggcta tttgaacttg ctcatggagg gactcttttt
     6241 ttagatgaaa taaatagtct atcctttaat attcagacaa aactattaag agtaatagag
     6301 gaacgtcaaa taatgagaat tggttctgat tatataatac ctctggatat aaggattata
     6361 gcagcaacga acgaatcttt aaccgaaaaa atagttatgg gtacatttag agcagactta
     6421 ttttatagat tgagtagttt agagataaat atacctcctt taagagacag aagagaagat
     6481 ataatacctt tgtttaacaa ttttgtaaat gaagtattaa aggatgatgg tctcaatgga
     6541 ataaacagta tagatgaaaa ttttgttttg actaaagatg aaatggataa gctatataat
     6601 tatagctggc ctggaaatgt aagagagtta aagacaatag ctcaaaaata tgttgtgaca
     6661 ggtaaaatta aattgagaca agatagagat tttaaaacta aaaaattact tcctaactct
     6721 gaagtagaca aatttaattc tgaaacgaca gcaagtgtag aagtccaaga tgaatctata
     6781 aatataagta agataaatga tggtaagata agtatagata taaaagaagt aaataagtat
     6841 gttgaagaaa aaataataag tatgcttttt gctcaaggac tttctaaaaa tgaagttgca
     6901 caagttcttg gaataagcag gacatcttta tggaagaaat acaataaaaa tatttaaata
     6961 taattacata taaattattt tgaagtaatg ttaatttttt aaaacaagtg ttaagaaaaa
     7021 ttaacattac ttttttgcaa atttttattt ttttagacag gcatatatat ttataagaaa
     7081 aatagttaaa acacttaatt taataatgaa aaaaataata attctaaaca aaatcaattt
     7141 tacataaagt gggatttatt ggcatagtaa ttgcttaata tataatgtaa gagtaaatac
     7201 aattttaggg gtgatggtat gagtcaaagt atggttggta agcatgcaat gtggccaaaa
     7261 gaaaatgatg ttatatttag catatctggc agagctcaag cagcagagaa ggcttttggt
     7321 atggataatg taataaatgc aacaataggc gctctaatgg atgattcagg aaaattgata
     7381 acaatgaaaa ctgtatatga agaatataaa gcattagata actgtgagat aggtgcatat
     7441 gcagctcttg aaggtcaacc agattactta gaagcagtta aaaaagtatt tttcagagat
     7501 tacttaccag aaggacatat aagagtatta gcttcaccag gaggaagtgg tgcaataaaa
     7561 ttagctgtat ggaactatac taatgaagga gatgaggtat taacatctga ttggttctgg
     7621 agtccatatg taagtatagc tgaagaggct aatagaaaag tagttaatta ccaacttttt
     7681 gatgaaaata gaaggtttaa ttttgagtcg tttaaagaaa aatttgtaaa tatagcagaa
     7741 aaacaaggta gagtctttac aattataaac acaccagcgc ataatccaac tggatatagt
     7801 gttgcagatg atgagtggga taaaatatta gacttatcaa aagaagtcgc aaaagataaa
     7861 gacaaaaaga taatattttt cgtagattca gcatatattg actttgcagg tgatgatgat
     7921 gtatgtagaa agttttttaa gaaattttct aatttgcctg aaaacgttct cgtattggtt
     7981 ggattcagta tgtcaaaagg atttacagct tatggtatga gaatgggggc tataatttgt
     8041 atatcttcaa gtgaagatgt agctgaagag ttccattact catgtgttca ttcatgtaga
     8101 gcaaattggt caaattgtaa tagaagtgcc atggcagtat tatctaacat tgtaaatgac
     8161 cctaaaaaat tcaaagaata tgaggatgaa aaagaaatat acaaaaacat gcttactaga
     8221 agagcagatg tatttgtaaa agaagctgaa agagtaggat tagaaattct tccatatata
     8281 gcaggtttct ttgtaagtat accatgtgat aatccaaaag aagtatgtga agaattgact
     8341 aaacataatt tatttgcagt tccattaaaa atgggtttaa gatttgcagt ttgtgcagtt
     8401 tctgaagata aatgtaagaa agcaccaagt attataaaag aagcacttga aagtttagaa
     8461 gtaaaaataa ataattaatt tcattgaaca aattgggtgg actatgaatt taataattgt
     8521 agatggaggg aattttatat gaaacaattg atgacaggta atgaagcaat tgcacgtgga
     8581 gcctatgagg ctggtgttaa atatgcatct gcatatccag ggactccaag tacagaaata
     8641 ttagaaaata tagcaacata taaagatgca atagttgcag agtgggctcc aaacgaaaag
     8701 gtagcacttg aggctgctat agggggttca attgcaggag caagaacaat ggcttctatg
     8761 aaacatgttg gtttgaatgt tgcagctgat ccaatattta cttatgcata tacaggggta
     8821 aatggtggta tggtacttat tacagcagat gagccgggaa tgcattcatc tcaaaatgag
     8881 caagacaata ggatgtatgc aaaatttgca aagatacctt tatttgagcc atcaactagt
     8941 caagaagcta aagatatgat aaaagaagct tttgaagtta gtgaaaaata cgatacacca
     9001 gttttatata gagttacaac aagactttgt cattcaaaag gattagtgga gtgctatgat
     9061 agagaagaag ttgaaattaa agagtatgtt aaaaatgcta agaaaatggt aactgttcca
     9121 gcaaatgctc aaataagaag gggcgttgtt gaagaaagaa tggaaattct taaaaaattc
     9181 tcaaatgaaa ctgatttaaa ctactatgaa ataaatgata caaaaatagg tgttatagcc
     9241 tctggtatgt gctgtaattt tgccaaagaa gtttttggaa aaaatgcatc atatatgaaa
     9301 ctaggattta caaatccatt accttatgaa aaaataaaag aatttgcaga aaaagttgat
     9361 aaaatatatg ttatagaaga aaatgaccca tttatagaag aacaaataaa agcatatgga
     9421 atagattgta ttggaaaaga tgtaatacca ccatatggag aaatgacacc agatgtttta
     9481 agaaaagcta tatttggaaa aacaaatgat actatagaat acaaatctga attagtaaca
     9541 ccaagaccac caagtttctg tgcaggatgt ccacatagag gattcttcta tgaacttgga
     9601 aagagaaaaa atttaatagt tggtggagat ataggttgtt atactcttgg atttgctcct
     9661 ccatataatg gaatagatta tgttgtttgt atgggttctg cttttggaac tgcacatgga
     9721 gctcaaaaag ttcttaatat gaaggatgat aatgaaaaaa gattggtagg tgtacttgga
     9781 gactcaacat tcttccatac aggaataaat ggacttttag atgttgttta caatagaggt
     9841 aattctattt cggttatatt agataataga ataactggaa tgacaggtca ccaagaaaac
     9901 ccaggttcag gatatacttt acaaggtgct aaaactaaag aagttgatat agaaggatta
     9961 gttaaagctt gtggaataga acatgtaaga gttataaacc ctaataattt aaaagaagta
    10021 aatgaagcac tagattgggc tttagctata gaagatgaac catcagtaat aataacaaga
    10081 tggccatgtg tacttaaaaa attctctaaa gaagatatag aagaatttaa taatccattt
    10141 aagactaagt gtaaagttga ccatgataaa tgtataggat gtaaattatg tttgaaaact
    10201 ggatgtccag ctctatcatt tgataaagag aataagttat caaatataga tagaaatcag
    10261 tgtgtaggat gtggagtatg tgcacaagtt tgtccaaaac aagcaatagt taaggaggaa
    10321 aaataatatg actaagagta tacttttggt aggtgtagga ggtcaaggaa ctatacttgc
    10381 tagtaaatta ttaactatgg gattgatgga agctggatat gatgtaaaga tgagtgaaat
    10441 acatggaatg agtcaaagag gaggttcagt ttcctcacaa gttagatatg gagattgtgt
    10501 tcattctcca gttatagaaa ttggtggagc agatatatta gtatcttttg aaaaaatgga
    10561 agctttaaga tggttcaatt atttaaaacc agaaggaaaa gctttagtaa ataatcatag
    10621 gatagattct atgactgttt taatcggagg agctgaatat caagaaagtg atatagatgc
    10681 agagttaagt agattaaatg caaaagttat aaatgcagct gataaagctg aagaactagg
    10741 aaattcaaaa ataatgaatg ttattttatt aggatgtcta gttaaatcta tgcaattaga
    10801 gtctattgat tgggaaaaaa ttataagtga aaatgttaaa ccaaaatttg ttgaattaaa
    10861 cataaaagct ttccatgaag gtatggaaat ggttagcaaa tagtttataa tagatataaa
    10921 tagactatag aaattaaggt gaaaggaaga aaattatgac ttacagaata ttagccataa
    10981 atccaggttc tacttctaca aaaatagcag tatatgatgg agaagaacaa attcttgtga
    11041 agacgataga ccatccagct gaagagattg caaaatataa tactatacaa gaccagtttg
    11101 aaatgcgtaa ggaagcagtt ttgaatattc ttaaagaaaa tagtatagac ttaaaatctc
    11161 ttagtgcaat agtaggaaga ggtggagttt taccaccagt aaaatcagga gcatatttag
    11221 taaatgaaga aatgattgat gtactaagac atagaccagt acttgaacac gcttccaatt
    11281 taggtgctgt tgtggcacat gcaatatcag aacctcttgg aatcaactca tatatttatg
    11341 attctgttgc agtagatgag cttatagatg tagcgagaat atctggactt tgtggaatgg
    11401 atagatcaag tgcagggcat gcattaaata ctagagcaat ggctttaaaa tatgctaagg
    11461 ataaaggaaa agattataag agcttaaact taatagtagc tcacattggt ggaggagtaa
    11521 gtatttatct tcatgaaaaa ggaagaatgg ttgatatgct atctgatgat gaaggaccat
    11581 tttctccaga aaggtcagga agagtacctg ctacaaaatt agtggctgcc tgttattcag
    11641 gtcaatattc agaaagagaa atgactaaaa agataagagg taaaggtggt atagtttcat
    11701 acctaaatac tgtagatgct agagaagttg aaaaaatgat agcagaagga aatgaagaag
    11761 caaaaattat ttatgaagca atggcttatc agttagcaaa aggtattgga gagttagcaa
    11821 ctgtagtaga tggaaaggta gatgctataa ttataacagg tggaattgca tattctgaaa
    11881 tgtttacttc aatggttaaa aagaaagttg agtttatagc accagtagaa attatggcag
    11941 gagaaaatga gttggaatca cttgcttttg gaactttaag agtactaaat ggagaagaag
    12001 aagctagaat ttatagtgaa aattaataag aaaaactaaa aatgtttata tactaaaaaa
    12061 gtggtatatc cgttttttat acacgaaagc ttagagtata ggtgaaaatg tataaaattt
    12121 ggattgccac tttttataat taaaatttta tcttaaccta ataaataatt ctgtaaaatt
    12181 tcgtatttat atatatattt tattggtatt tattgttatt attattatga ggagtgattt
    12241 ttgatgggaa aaataagttt aaaaagtatg ataagattat tttcaggttt ttttatattt
    12301 gcaatttctt ctgtgcttat gattaatgca catgtaggtc ttatgccttg ggatgtactt
    12361 caccaaggtt tatctataaa gctaggcata actataggtc aggcttctat aatggtagga
    12421 gttgtaattg ttatattgga tgcagttttt ggtgaaaata taggatgggg tactttactc
    12481 aacatgacat ttataggaat ttttattgat ttagtaatat tttctggagt gatacctcat
    12541 gcaagcaaca cttatatagg tgtgtttatg gttgttatag gaattatttt ggcagctata
    12601 gcaagttttc tatacttagg tgtttgttta ggaagtggac cgagagatgg gcttatgatt
    12661 gctcttcaga aaaaaactaa taaatctgta cgtcttgtta gaacaattct tgaaatacta
    12721 gcattagttg ttggatggtt attaggaggt tctgtaggta ttggtacgct tgtaagtgct
    12781 ttaggtttag gatatgtatt acaaatagtg tttagaatat ttaaatttga cacaaaattg
    12841 ctgaaacata gatttattat agatgatata agagaatgga aggaaaagaa aagcaatgaa
    12901 cataaatgta aatcaagtat tgtaataaaa aatgagcaga attaattatg ctaaaatata
    12961 tttgtagttt atctaattta tagtaaagtc aaggagattt tgaaatggaa ttatgggatt
    13021 tatataatgc tgatggaatt aaaacaggaa atgttataaa aagaggtaat tctattgaag
    13081 aaggttatta ccatttggca gttgaagtgt ggattttaaa tagtaactct caaattttaa
    13141 ttcagaaaag gtctaaaagt aaaaaaacat tgccaaatat gtggggaatg acaactggat
    13201 gtattgtttc tggggaagac agtttagaag gagcaattcg cgaagcaaag gaagaaatag
    13261 ggatagatat aactaaagat gagatgaaag tttttagaag tatgattcat gaagatactc
    13321 tttgggatgt atacttagtt aaaaaagaat atgatatatc taaagctatt ttacaggagg
    13381 aagaagtaag tgatataaag tgggtaagca cagatgaaat aagacaatta ctaaaagagg
    13441 gtttgttttt tgaatatcca gagatatacg aattgctata tgagatagat aataataaat
    13501 taaatatatg atgtagagca gatttcagta taagtaagat tatttaaaat gtatcttctc
    13561 taaattttaa tattgtttta tgaaaattaa taagttattt ttagtatttt tctacttgtt
    13621 ttttgagatt aaaataaaaa gggcagtcat tataaagtca taaaaatttg gggaggattt
    13681 aaactatgga agataacaaa ggattggcac gtgacagttc aaaatataaa attgtttcta
    13741 tatctgtcgt agtaattatt ctaattacat tttggtatat gttaaatatg gtgcttttaa
    13801 catttattat gacttttatt ttctataact tgcttgtagc cacaagaaaa cgaattaaga
    13861 agttttcttc tttaaatatt ccagattcat tgataattat agtgttatat gcactatttg
    13921 ctatattgtt agtattgatt agctatgcag ttgtaccaat aattatagtt caacttacag
    13981 agttgagtag ggtttttagt gattttgatg taaatcaatt tgctcaatct ttaggaccga
    14041 aattgtatcc tattgtctca aagctggatt ttaataaata tatttcacaa gcagggctac
    14101 ttatagcatc aacagcaaca aaagttggaa gttttggagt aaatatatta ttggcatttt
    14161 tactgagttt actcctttta cttgaaaaaa atgaaattaa aaattttgga gataaacttt
    14221 ctgatagtaa gatttctttt atttataatt cgcttgtatt ttttggaaag agctttgtta
    14281 aaaattttgg tgaagtaatg aaagtacaag ttatgattgc ctttataaat tctgttgttt
    14341 ctatgatttt cttaggtttc atgggattcc ctcaaatatg ggctttagga tttatgattt
    14401 ttgtgttggg tcttattcca gtagcaggag ttatagtgtc tttgattcca ttaactgtaa
    14461 tagcatttaa tacaggagga ataacaaagg tttttggagt tttacttatg atttgtatag
    14521 tgcatgcagt agaaacatac atattaaacc ctaagttgat gtcgaataga actaaacttc
    14581 cagtttgttt tgtttttatt attttacttg taggagagca ttatttaggt gtatggggat
    14641 tactaattgg agttccaata tttatgttcc ttatggatat tctgggtgtt aaatttactt
    14701 ctaggtaata tattaaaata ataatttatt tttatatata aattatgaat tgttttaaaa
    14761 aagaacctat aaatagacat cattaataaa gacatctatt tgataggttc aattttgctt
    14821 tgtataaata ctataaaact aaataaaaag aaatttattt catttcaaaa gtttcacaac
    14881 ttgcatttct ttcatttata tgaacttttt cagctttgca aagttcattt ttattgtatt
    14941 tacattttac agcttcacaa tgaatgttat ctgtatcaac ttggttagtt tcattagaac
    15001 gactagttat accagatgta tatttgtctt caaaagaagc acaataagta ttttctgtag
    15061 tagttgcttg cattccatct acttttatac taccagcata acaatatcca ctagtattat
    15121 aagcacaatt agtagcagca caatttaaat tattattttt cataaataac ctcctaagtt
    15181 ttatttaaac ttattatgtg aagttatcta aattttatac acttatttac aattacattt
    15241 atcacattta acaactacat ctttggaagt ttcccttcct aaatttttat taacatgata
    15301 cacatcaaca ttttcagttg atccacatat agaacattta aaattgtttt ctagtagggt
    15361 tctttcttta aataacttcc aatacataga attgatgtaa tcattataat ttaccttgag
    15421 gcatgtctca agaaagttta tgtatagatt gtctaaatct cctgtattta attctgtata
    15481 ttctggttca aaatattcac aacatcctct aaaatcgggc tttgtatctg aaataacaca
    15541 tctaatatca tatctatagt ctaggctagg ccccttaaat gaaagatgtg tgcaaaaaga
    15601 acaagatatt tctggtagat ttttttcaag ttcatgtaat ttattattca taagtattcc
    15661 tccaaaaaat ttattttaag tatataaatg aatagattat tttaaaactt ttatttattt
    15721 atattagaga tttaggataa atattccaat aaggagtaaa tacttaagta tttattactg
    15781 tattaaataa aaaagaggca aatctaactt gagattgcct acttttttat cattttttaa
    15841 taaaatttat tctaagtatt attttattta gatttagtca gccaacttag tatctgcttt
    15901 gctttttaac tttctaccag agcgtacagt taaaatagca tataatacag taaatatggc
    15961 agctaaaaca tatgatatat ttatgtttaa gttaaatcca atttgagcat taaatatata
    16021 actgaatata acaaaggaat aaaacatacc aggaattaag cttataatat aattcttttc
    16081 ttttattata aggtaaactg taatcattgc aaatgcaaat atggcaatag tttgattact
    16141 ccaagagaag tatctccaca atatattaaa tccagaagca ctaagctttg caaatataag
    16201 gatggctatg acaggaataa atatacagat tgacaatata acacgatgtt tcttttcctt
    16261 ttggtcataa tgaaagtaat ctgcaagcat taatcttaaa gatcttaaag ctgtatctcc
    16321 agaagttatt ggtaaaacaa ttacacctat tatagctatg ataccaccaa tagaaccaag
    16381 taaatctctt gcaaccaaac ctattacatc aggagaacct actaattctt taggtattcc
    16441 tttattgtaa atacccatag cagctgcagc ccaaatcatt gcaattaaac cttctaaaat
    16501 catcatatta taaaaagtag tttttccttc tttttcattt gaaactgatc ttgctataag
    16561 agtagcttga gttgagtgga atccagaaac aataccacaa gcaactgtaa caaagaatgt
    16621 aggaataaga ggaataccat caggatgaat acctttccaa tttgcaagac ttaaattagc
    16681 taaatcataa ccttgtgtaa agattccaat tccaacacca gcagcagata aaagtagaat
    16741 agctccaaat attggatata cctttcctat gattttatca attggaaata gtgttgcaca
    16801 caagtaatat agaaagataa gtccataaac tatccagaca actggattat ttatagtcga
    16861 tttcatattt aaaatttgag taacaactaa atccccaggt gtataaataa aaactgcacc
    16921 aactaaaagc attaaaagac aaaggaatat attatagact tgaaatactt tattacctaa
    16981 aaatctactg ataagagaag gcatttgtgc tcctttttct cttatactaa tcattccact
    17041 catatagtca tgcaaagctc ctccaaatac acagccgata ggtattagta tgaatgctat
    17101 tggaccaaac aatatacctt ggataggtcc tagaattggt cctgtaccag ctatattaag
    17161 caactcaatt agactatttt tccatttttt cattgggaca tagtctactc catcactttg
    17221 agcaagtgca ggtgtttttc tgtcatctgg gccaaatact ttttcacaat aagcaccata
    17281 taaagcacct cctattagta atatagccaa gccgattaag aatgttgtca taatatcacc
    17341 ccatttataa aattttttgg tctttgtata catattatct aaatgtgtag atttatattg
    17401 aatttaattc tggacaacct acaaattcta taaagtatta caactaatat aatttcagaa
    17461 ataaatacat atacataacc atgtacttag tatacaggta tttggtaata ttttcaatgt
    17521 tatagcaata aagaatataa acaaaattct gataatattt tacaaaaaaa ttaaaaaata
    17581 taaaaattag tttaaataga tggagaaaaa aggaaaatca attgagaata taaatgtcta
    17641 tatcttaaca atattaagag gtttaaaatt cttgaagaaa gaatagtcaa gtgatataat
    17701 gggtttacag gtgtatatac actagtacat atagaaagcg gtgcaattat ggataaagat
    17761 ttaacatatc aaatcaaaga gctaaagaaa gaaaaaaatg caataatact tgcccatttt
    17821 tatcaaccac cagaaataca agaattagca gatgcagttg gtgactcata ttatttaagt
    17881 gagattgcta gagattgtaa agaagaagtg gttgtatttt gtggagtaag gtttatggga
    17941 gaaagtgcta agatattatc acctgaaaaa actgtattga tgccagtaag taacgctggg
    18001 tgtgctatgg cagatatggt agatgaagaa ggcgtgataa aattaaagca acagtatcca
    18061 aatgctttgg ttgtatgtta tataaactct acagctaaag ttaaggcaca ttgtgatgtt
    18121 tctgttacat cttctagtgc aattaaaata ctagaaaaca tagataataa agaaattata
    18181 ttccttccag ataaaaattt aggaggatat attgcagaac aatttccaga taaaaacttt
    18241 atattttggg acggatattg taaataccat aacaatataa gagctgaaga aataattgag
    18301 ttaaaagata agtataaaaa tgcagaagta ttagtacatc cagaatgtaa aaaagaaata
    18361 agagatttag gagattatgt aggtagtaca tcaggaataa taaaatatgc tactaatagt
    18421 aaaaacaaag actttataat agcaactgaa gaaggtatat tacatgaatt aaagaaaaat
    18481 aatcctaata aaaactttta tattcctggt ggaaaaatac tatgtacaga tatgaaaaag
    18541 acaacattag aaaatttata tagtacactt aaaaatatgg aaaatgaagt tattgtagaa
    18601 gatgaaatca tggaaaaggc tttaaattca ctgcttaaca tgcacaaatt agcagaagga
    18661 tagagggata gacttatgaa tttagaacaa gatgttttaa tagttggaag tggagtttca
    18721 ggattatact gtgcattaaa tttagataaa agtttaaatg tgctagtagt atcgaaatca
    18781 acaattgaaa acaacaatac atatcttgca caaggtggaa tttcaactgc tagaaatatt
    18841 gatgatatag aatcctttgt agaagataca atgaaggctg ggcagtataa aaatagagtt
    18901 gaagcagttc aagtacttgc agatgaatct atagaaaatg taggtcagat agtagaatat
    18961 ggtatgcctt tagacaaaga aaatggagag atagattaca caagagaagg agcacatagt
    19021 gtaaatcgaa tagttcatag taaggataat actggagaag ttgtttttaa aactttatta
    19081 aaagaagcta aaactagaga aaatataact cttatagaag atgcatatct tttagatata
    19141 ttaaaagatg gcaataaatg tataggagct agaattttta aatctaaaaa agagattcat
    19201 gttttttcaa aaatagtagt tttagctaca ggtggaattg gtgggctttt taaaaattct
    19261 actaatcaaa gacatcttac aggtgatgga attgcaatag ctttaagaaa taatataaaa
    19321 atagaaaatt tagaatatat tcaaatacat cctacagcct tttatgaaga aaataatgaa
    19381 ggtaggagaa tgcttatatc tgaatcatta agaggagagg gagctaaact tttaaataaa
    19441 aataaagaaa gatttgtgga tgaacttctt cctagagatg tagtttcaaa agctattttt
    19501 gagcaaatgg aaaaagataa attaccttat gtgtatttag atgcaacaca tttagatagt
    19561 gaatatttaa ttaatagatt ttcttttatt tataatgaat gtttagctag aggaacagac
    19621 ataacgaaag aatgtataaa ggtatctcct gcacaacatt attttatggg cgggatacat
    19681 gtagatttgg attcaaaaac atctatggaa aatttatatg cagttggtga aattagttgt
    19741 acaggtgttc atggagccaa tagattggca agtaattcat tacttgaagg gcttgtattt
    19801 tcaaaaagag cagctaaaaa tataaactca gttatagata atgtaaaagt taagtttata
    19861 gatgtaccag atatggatat agatatagaa caggtaaaaa aagaaaataa aatattagtc
    19921 ataaaagaaa ttgaaagaac aagtgaggat tttggcgatg aattatttga ttattgatag
    19981 aatgatacag gatgctttga tagaagatgt tccaagtgaa gatataacaa ctaattctat
    20041 tgtggatgaa aattctaaat ctactgtaga tttaatttgc aaacaagatg gtataatagc
    20101 tggcttaggt gtatttaaga gagtttttga gatacttggt gatgtagatg taaaattata
    20161 taaaaatgat ggtgatagag taaagaatcg tgaaaagata gctgtattaa caggtagtac
    20221 tagaaactta cttgtaggag agcgtgtagc attgaattat ttacagagaa tgagtggtat
    20281 agctactatt acaagtaggt atgttgaaaa gcttgaaggt acaaatacaa aattattaga
    20341 tagtagaaaa acgataccta atcttagaat attagataag tattctgtca aagtaggtgg
    20401 aggatgcaat catagattta atttatctga tggaatattg cttaaagaca accatattgg
    20461 ggctgctggt agtgttaaga aggcagttga attagctaga aaaaatactt cttttgttag
    20521 aaagatagaa gtggaagtgg aaactttaga catggtaaaa gaagcaatag aagccaatgc
    20581 agatataata atgttggata atatgagttt ggaaatggca aaacaagcag tagatattat
    20641 aaatggaaga gccattgtag aattttccgg aaatgtaaat ttagatacta tagaagatat
    20701 aggaaaaatt ggtgtggatg ttgtttctgt aggagcgctt acacattctg ttaaagctct
    20761 cgatataagt atgaagaatt tgagaaatat ataaatttta tattgaaata aacttaatta
    20821 aaatagactc cttaaatttg ttttataaat ttaaggagtc tattttttat tcattaaaat
    20881 taataaaaat agaaaaaaat caat
//
LOCUS       DAEOSP010000051        20762 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.051, whole genome shotgun sequence.
ACCESSION   DAEOSP010000051 DAEOSP010000000
VERSION     DAEOSP010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.051"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            370..681
                     /gene="rpsJ"
                     /locus_tag="KRH54_001528"
     CDS             370..681
                     /gene="rpsJ"
                     /locus_tag="KRH54_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBG8579195.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            773..1402
                     /gene="rplC"
                     /locus_tag="KRH54_001529"
     CDS             773..1402
                     /gene="rplC"
                     /locus_tag="KRH54_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBG8579196.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1432..2055
                     /gene="rplD"
                     /locus_tag="KRH54_001530"
     CDS             1432..2055
                     /gene="rplD"
                     /locus_tag="KRH54_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBG8579197.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2055..2345
                     /gene="rplW"
                     /locus_tag="KRH54_001531"
     CDS             2055..2345
                     /gene="rplW"
                     /locus_tag="KRH54_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBG8579198.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2375..3205
                     /gene="rplB"
                     /locus_tag="KRH54_001532"
     CDS             2375..3205
                     /gene="rplB"
                     /locus_tag="KRH54_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBG8579199.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3240..3521
                     /gene="rpsS"
                     /locus_tag="KRH54_001533"
     CDS             3240..3521
                     /gene="rpsS"
                     /locus_tag="KRH54_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBG8579200.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3553..3888
                     /gene="rplV"
                     /locus_tag="KRH54_001534"
     CDS             3553..3888
                     /gene="rplV"
                     /locus_tag="KRH54_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBG8579201.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3911..4726
                     /gene="rpsC"
                     /locus_tag="KRH54_001535"
     CDS             3911..4726
                     /gene="rpsC"
                     /locus_tag="KRH54_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBG8579202.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4762..5193
                     /gene="rplP"
                     /locus_tag="KRH54_001536"
     CDS             4762..5193
                     /gene="rplP"
                     /locus_tag="KRH54_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBG8579203.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5195..5398
                     /gene="rpmC"
                     /locus_tag="KRH54_001537"
     CDS             5195..5398
                     /gene="rpmC"
                     /locus_tag="KRH54_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBG8579204.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5422..5676
                     /gene="rpsQ"
                     /locus_tag="KRH54_001538"
     CDS             5422..5676
                     /gene="rpsQ"
                     /locus_tag="KRH54_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBG8579205.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5702..6070
                     /gene="rplN"
                     /locus_tag="KRH54_001539"
     CDS             5702..6070
                     /gene="rplN"
                     /locus_tag="KRH54_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBG8579206.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6094..6399
                     /gene="rplX"
                     /locus_tag="KRH54_001540"
     CDS             6094..6399
                     /gene="rplX"
                     /locus_tag="KRH54_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBG8579207.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6429..6971
                     /gene="rplE"
                     /locus_tag="KRH54_001541"
     CDS             6429..6971
                     /gene="rplE"
                     /locus_tag="KRH54_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBG8579208.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6989..7174
                     /locus_tag="KRH54_001542"
     CDS             6989..7174
                     /locus_tag="KRH54_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBG8579209.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7207..7605
                     /gene="rpsH"
                     /locus_tag="KRH54_001543"
     CDS             7207..7605
                     /gene="rpsH"
                     /locus_tag="KRH54_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBG8579210.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7636..8178
                     /gene="rplF"
                     /locus_tag="KRH54_001544"
     CDS             7636..8178
                     /gene="rplF"
                     /locus_tag="KRH54_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBG8579211.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8214..8582
                     /gene="rplR"
                     /locus_tag="KRH54_001545"
     CDS             8214..8582
                     /gene="rplR"
                     /locus_tag="KRH54_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBG8579212.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8603..9112
                     /gene="rpsE"
                     /locus_tag="KRH54_001546"
     CDS             8603..9112
                     /gene="rpsE"
                     /locus_tag="KRH54_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBG8579213.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9127..9312
                     /gene="rpmD"
                     /locus_tag="KRH54_001547"
     CDS             9127..9312
                     /gene="rpmD"
                     /locus_tag="KRH54_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBG8579214.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9345..9788
                     /gene="rplO"
                     /locus_tag="KRH54_001548"
     CDS             9345..9788
                     /gene="rplO"
                     /locus_tag="KRH54_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBG8579215.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9832..11100
                     /gene="secY"
                     /locus_tag="KRH54_001549"
     CDS             9832..11100
                     /gene="secY"
                     /locus_tag="KRH54_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBG8579216.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11117..11767
                     /locus_tag="KRH54_001550"
     CDS             11117..11767
                     /locus_tag="KRH54_001550"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBG8579217.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11768..12514
                     /gene="map"
                     /locus_tag="KRH54_001551"
     CDS             11768..12514
                     /gene="map"
                     /locus_tag="KRH54_001551"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBG8579218.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12536..12805
                     /locus_tag="KRH54_001552"
     CDS             12536..12805
                     /locus_tag="KRH54_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HBG8579219.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12843..13061
                     /gene="infA"
                     /locus_tag="KRH54_001553"
     CDS             12843..13061
                     /gene="infA"
                     /locus_tag="KRH54_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBG8579220.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13097..13210
                     /gene="rpmJ"
                     /locus_tag="KRH54_001554"
     CDS             13097..13210
                     /gene="rpmJ"
                     /locus_tag="KRH54_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBG8579221.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13363..13734
                     /gene="rpsM"
                     /locus_tag="KRH54_001555"
     CDS             13363..13734
                     /gene="rpsM"
                     /locus_tag="KRH54_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBG8579222.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13770..14168
                     /gene="rpsK"
                     /locus_tag="KRH54_001556"
     CDS             13770..14168
                     /gene="rpsK"
                     /locus_tag="KRH54_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBG8579223.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14200..14823
                     /gene="rpsD"
                     /locus_tag="KRH54_001557"
     CDS             14200..14823
                     /gene="rpsD"
                     /locus_tag="KRH54_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBG8579224.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14901..15848
                     /locus_tag="KRH54_001558"
     CDS             14901..15848
                     /locus_tag="KRH54_001558"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBG8579225.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15869..16210
                     /gene="rplQ"
                     /locus_tag="KRH54_001559"
     CDS             15869..16210
                     /gene="rplQ"
                     /locus_tag="KRH54_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBG8579226.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16330..17163
                     /locus_tag="KRH54_001560"
     CDS             16330..17163
                     /locus_tag="KRH54_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG8579227.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17151..18017
                     /locus_tag="KRH54_001561"
     CDS             17151..18017
                     /locus_tag="KRH54_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBG8579228.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18011..18814
                     /locus_tag="KRH54_001562"
     CDS             18011..18814
                     /locus_tag="KRH54_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBG8579229.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18846..19577
                     /gene="truA"
                     /locus_tag="KRH54_001563"
     CDS             18846..19577
                     /gene="truA"
                     /locus_tag="KRH54_001563"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG8579230.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19697..20128
                     /gene="rplM"
                     /locus_tag="KRH54_001564"
     CDS             19697..20128
                     /gene="rplM"
                     /locus_tag="KRH54_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBG8579231.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVIVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20157..20549
                     /gene="rpsI"
                     /locus_tag="KRH54_001565"
     CDS             20157..20549
                     /gene="rpsI"
                     /locus_tag="KRH54_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBG8579232.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8294 a         2451 c         4320 g         5697 t
ORIGIN      
        1 ctttactaaa ctaatggatt taatataaaa aaagaggggg gtaactcctc tcttttttgt
       61 tttttgttga caagtagcgc taaagcatat atactaaact agtgccttga gacagattaa
      121 caatcaagtt taaggttatg atgttgtaat agaaaataaa attgaaaaaa acagaaaaaa
      181 aacttgaaaa aatgtaacag atgatataaa atataatagt gtgcttgaga gattaagaaa
      241 ataataatta aatcctagtg aaatacgaac tgatatcgat ttaaaagtta caacacaccc
      301 ggagcagtgt atcggtattg gttgtacgca acttgaaaat taaacacgtg ataaaggagg
      361 gaaaacaaaa tggctaagaa tgaaaaaata agaataagat taaaatcata tgatcacaaa
      421 ttattagatt tttcagcagg taaaatagtt gaaactgcta aaaaggctgg atcacaagtt
      481 tcaggacctg tgcctctacc aacagaaaaa caagttgtaa ctatattaag agctgtacat
      541 aagtacaaag actctagaga acaatttgaa ataagaactc ataaaagact aatcgacata
      601 gctaatccaa cacctaagac agttgactca ttaatgagat tagacttacc agcaggtgta
      661 gatatagaaa taaaattata agaaccccta agatattatc ctatagatga ttatcttaga
      721 atgattactc taaaagatgt aatccgctgt aagaattata ggaggtgcta atatgaaagg
      781 aatattaggc aaaaaagttg gtatgactca aatattcact gataaaggtg tagttatacc
      841 tgtaacagct gttgaagcag ggcctatggt cgttactcaa ataaaaacag ttgataaaga
      901 tggatacaat gctatacaaa taggttttga agatgctaaa gaaaaagctt taaacaaacc
      961 taaaaaagga catttagctg ctgctaatgt tttaaagaaa catttaaaag aatttagagt
     1021 agattctgta gaaggataca cagtaggaca agaaataaaa gctgatgtat ttgaagcagg
     1081 tgcaaaaata gatgttactg gaataagtaa aggtaaggga ttccaaggtc caataaaaag
     1141 acatggacaa agtagaggtc ctgaaactca cggttctaga taccacagaa gaccaggttc
     1201 aatgggagct tgttcttacc caggtagagt atttaaaaac aaaaaattag ctggacatat
     1261 gggaagcgta aaggtaacag ttcaaaactt agaggttgtt aaagtagatg ctgacaagaa
     1321 ccttatatta gttaaaggag ctataccagg agctaaaggt tcagtagtaa ctataaaaga
     1381 agctataaag gtttctaaat aatgactaac ctagagaaag gaggaataac aatgccaaaa
     1441 ttaaatgtac taaatgttag tggacaaaac gttggagaaa tagaattatc agattctata
     1501 tttggcgtag aagttaatgg acatgtttta tacgaagttg taaaaaatca attagcaaat
     1561 aagagacaag gaactcaatc tgctaaaact agagcagaag ttagaggtgg cggaagaaaa
     1621 ccttggaaac aaaaaggaac aggtagagca agacaaggtt ctacaagatc tgtacaatgg
     1681 gtaggtggag gagttgcttt tgcacctaaa ccaagaagct acaaatatac attacctaag
     1741 aaggtaagaa gattagctat gaagagtgct ttatcttcaa aagttcaaaa tagtgaagtt
     1801 atagtattag atgcattaaa catggatgct cctaagacta aagaatttgc tcaaatatta
     1861 aacaatataa atgctgctaa aaaagcttta gtagttatag ctgacaaaaa cgataacgta
     1921 ataaaatcag ctagaaatat agaaggtgtt caaactgcat tagttaatac tatgaatgta
     1981 tatgatatat taaaatacga ttcatttata ataactacag atgctgttaa aaaagtggag
     2041 gaggtgtacg cataatgact aatccacatg atgtaataat aagaccagtt gtaactgagc
     2101 acagtatggc tgaaatgggt gaaaaaaaat acacttttgt tgtggctaaa gatgcgaaca
     2161 aaactgaaat aaaaaaagca gtagaaaaag tattcggagt tagtgttgat aaagtaaata
     2221 ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac tcaaggaaga acatcaagct
     2281 ttaagaaagc agtagttaaa ttaactgctg atagtaaaga aatagaattc ttccaaggaa
     2341 tgtagtatta taacaaacga aggagggaaa acagatggct ataaaaaagt ttagaccaac
     2401 ttctcctgcc ttaagacaaa tgacagtttt agtttctgat gaaataacat gtaatcaacc
     2461 agaaaaatct cttttagtta acttaaagaa aaatgctggt agaaacgtac atggtagaat
     2521 aactgttcgt catagaggtg gaggacaaaa aagaaaatat agaataatag attttaaaag
     2581 agataaagat ggtatacctg ctaaggttgc tactatagaa tatgatccaa acagaactgc
     2641 aaatatcgca ttattaaact atgctgatgg agaaaaaaga tatatattag ctccagttgg
     2701 aattaacgtt ggagatacta tattatcagg attaggtgct gatataaaac caggaaactg
     2761 tttagcatta aaagatatgc cagttggtac tatcattcat aatatagaat taaagccagg
     2821 aaaaggagct caattagtta gatctgctgg agtttctgca caattaatgg ctaaagaagg
     2881 aaaaaatgct ttattaagat taccatcagg tgagatgaga ttagtaagca taaactgtaa
     2941 agctactata ggtcaagttg gaaatataga gcatggtaac gtagttatcg gtaaagcagg
     3001 tagaaaaaga catatgggaa taagacctac tgtaagaggt tctgtaatga accctaatga
     3061 ccatccacac ggtggagggg aaggtagatc tccaataggt agaccatctc ctgttactcc
     3121 atggggtaaa ccagctcttg gatacaaaac tagaaagaaa aataaagctt caaataaact
     3181 aatagtatca agaagaacta agtagtaaat ttatttttgc tcggaaagga ggaatataaa
     3241 tgtcaagatc aactaaaaaa gggccttttg tccatgcaag acttttaaag aagatagaag
     3301 ctatgaatgc tagtggaaat aaagaagtta taaaaacttg gtcaagatct tcaactgtat
     3361 ttccacaaat ggtagaaaac actatagctg ttcatgatgg aagaaaacat gttccagtat
     3421 atataacaga agatatggtt ggacataaat taggtgagtt cgttcctact agaactttca
     3481 aaggacataa ggatgacgaa aaatctaata agagaaaata attaaattat gactaagaaa
     3541 ggaggaataa caatggaagc aaaagctact gctaaatacg tacgtgtatc accaagaaaa
     3601 gcaggccaaa tatgtgacct tgttagagga aagaatgttg atgaagcatt agcaatatta
     3661 aagtttactc caagaggagc agcttcaata atagctaagg ttgtaaaatc agctaaagct
     3721 aacgcagaaa acaatcacga aatggatact gaaaaattat atatagcatc aatagttgct
     3781 aatcaaggac caacaatgaa gagattcatg cctagagcta tgggtcgtgc aactacaata
     3841 agaaaaagaa cttctcatat agaggttgtt gttaaagaaa aaaaataatt aatagatagg
     3901 agggaaagta atgggtcaaa aggttaatcc acacggctta agggtcggtg ttataaaaga
     3961 ttgggactca agatggttcg ctactgataa aaaagagttc ggaaacttat tattagaaga
     4021 ccataatata cgtaaattct taaagaaaag attatactca gctggagttg ctaagataga
     4081 aatagaaaga tcagctaaca aaataaaaat ggacttacac gttgctaagc ctggtgtagt
     4141 tataggaaga gctggtgcag gaatcgaagc attaaaagct gaattagaaa aaatgacaaa
     4201 gaaaactata atagttaata tagtagaagt aagaagtaca gataaaaatg ctcaattagt
     4261 agctgaaaat atagctttag ctatagagag aagggttgct ttcagaagag caatgaaaca
     4321 agctatacaa agagcaatga aatctggtgc taaagggata aaagtttctg cttcaggaag
     4381 attaggtgga gctgaaatgg ctagaactga aggttatagt gaaggaaatg tgccactaca
     4441 aactttaaga gcagatatag attatggttt cgctgaagct gatacaactt atggaaaaat
     4501 cggtataaaa gtttggatat gtaatggtga agttttacca actagagacg gtgtaaatcc
     4561 aagagaagaa agcagaaaga gcgatagaag agacaacaag agagataata gaagaaacga
     4621 tagaagagga aatgacagaa gaggaaatga taacagagga aactacagag gacaaagacc
     4681 tcaaggtgga tcaagacctc aaagaactga aaacaaagga aattaataat agttaggttc
     4741 aaggaaggag gaaaaatact catgttaatg ccaaaaagag taaagcgtcg tagagttcat
     4801 agaggaagca tggctgggca agctcataag ggtaataaag ttacttatgg tgagtttgga
     4861 ttagtagcat tagaagcttc ttggataact tctaatcaaa tagaagctgc cagaattgcg
     4921 atgactagat atataaaaag agggggaaag gtttggataa aaatattccc tcataaacca
     4981 gtaacaagaa aaccagcaga gactcgtatg ggtgcaggga aaggttctcc agaatattgg
     5041 gtagctgtag ttaagccagg aagagttatg tttgaattag caggtgtttc tgaagataaa
     5101 gcaagagaag ctatgagact tgctgcacat aaattaccaa tcaaatgtaa atttgttaaa
     5161 aaagaagatt tagaagtaaa gggtggtgaa taggatgaaa gctaaagaat taagagattt
     5221 aacaagcgaa gagctaatga acaaattaaa cgacttcaaa agtgaattat ttagcttaag
     5281 attccaatta gctactggtc aattagaaaa tacagctaga ataaagtttg ttaagaagga
     5341 tatagcaaaa gttaaaactg ttcttgctga aagaaagtta tacgaaacta gagcttaatt
     5401 tggaaaggag gctttttaaa catggaaaga ggaagaagaa aagttagaat aggccgtgtt
     5461 gttagtgata agatggataa aacaatagta gtagctgttg aagaattcgt acgtcatcca
     5521 ctatataata aacgtgttaa gagaactaag aaattcaagg ctcatgatga aaagaatata
     5581 tgtaacatcg gagatagagt taaaataatg gaaactagac cattatctaa agataagaga
     5641 ttcagactag ttgaagttgt tgagaaagtt aagtagtttt tgaagaagga gggattacga
     5701 tatgatacaa caagaatcac gtctaagagt tgctgataac tcaggagcta aagaactttt
     5761 atgtatacgt gttctaggcg gaagtaaaag aagatatggt aacataggcg acgttatagt
     5821 tgctactgtt aaaagtgcaa cacctggtgg agttgtaaaa aaaggtaagg ttgttaaagc
     5881 tgttatagta agaagcaaac aaggcgtaag acgtaatgac ggaagctata tatcttttga
     5941 tgaaaatgct gctgttataa taaaggacga taaaactcca gtaggaactc gtatattcgg
     6001 gcctgttgct agagagttaa gagacaatga attcatgaaa atagtttctc ttgctccaga
     6061 agtactataa ttaaggaggt gcaataggac atgatgcgtg ttaaaaaagg tgacactgtt
     6121 gtagttatag caggtaaaga taaaggtaaa aaaggttcag ttttaaaggt atatcctaaa
     6181 actagcaaag tgttagttga aggtgtaaat gtaataacta aacatcaaaa accaagtgct
     6241 atgaaccaac aaggtggaat aataaataaa gaagccccaa tacacatatc taatgtaatg
     6301 ccatttgatc ctgaaacagg aaaaggcgtt agagtaagat atgaagtaaa agatgggaac
     6361 aaagtaagag tatcagcaaa gagcggaaaa gaattataat aaatattgct tgaaaggagg
     6421 gaccttaaat ggcttctaga ttacaagaaa aatacatgaa agaagttgct cctgctttaa
     6481 tggagaaatt tggatacaaa aacgtaatgg agatacctaa gttaaataaa atagttatta
     6541 acatgggtat aggtgacgct agagaaaatc caaaaggatt agaaaaaggc gttgaagaat
     6601 tagaaatgat atcaggacaa aagcctgtta taactaaagc tagaaaatct gttgctaact
     6661 tcaaattaag agaaggaatg ccaataggaa caaaagttac attaagagct gacaaaatgt
     6721 tctactttat ggacaaatta gtttcagttt ctttaccaag agttagagac tttagaggag
     6781 ttaaccctaa tgcttttgat ggtagaggaa attatgcttt aggagttaaa gaacaattaa
     6841 tattccctga aatagaatat gataaaatag ataaagtaag aggaatggat ataatatttg
     6901 ttactacagc aaaaactgac gaagaagctc gtgaattatt aaaattatta ggaatgccat
     6961 tttctaagta agcaaaggag ggattcccgt ggctagaaaa gcgatggttg ttaagcaaca
     7021 aagaaagcaa aagtacgcta ctagagaata cactagatgt actatatgcg gaagaccgca
     7081 ttcagttttg aaaaaatttg gtatatgccg tatatgcttt agagaattag cttataaagg
     7141 tcaaatacct ggtgttagaa aagcaagctg gtagaagctt gattagaatg gaaggagggc
     7201 aacaaaatga caatgacaga tccaattgca gatatgctta ctcgtataag aaatgctaat
     7261 gttgttaagc atgaaactgt agatgttcca gcttctaata tgaagaaaga attagctaga
     7321 atcttattag aagaaggttt cataagaggt tatgatgtta tagaagatgg aaaacaaggt
     7381 atcataagaa tacaattaaa gtacggacaa gaaggcgaaa gagttataac aggtcttaag
     7441 aaaatatcta agcctggaat gagagtttac gctgctaacc atgagatacc aaaagtatta
     7501 aacggattag gaatatcagt tatatctact tcaaaaggaa tattaactga caagcaagct
     7561 agaaaagaaa atgttggtgg agaagtaatc tgctacgttt ggtaattaat atgaacaatg
     7621 taaggaggtg cgaccatgtc aagaataggt gttaagccaa taataatacc agcaggtgtt
     7681 gaagttacta tagctgaagg aaatttagtt acagtaaaag gtccaaaagg aactttaact
     7741 aaacaattaa gtgctgagtt aaacataaaa aaagaagaaa atacaataat ggttgaaaga
     7801 ccaactgata ataaaaaaca tagatcttta cacggactaa ctagaacttt attagataat
     7861 atggttgtag gtgtaaatac tggtttcgaa aagaaattag aattaaaagg tgttggatat
     7921 agagctcaaa aacaaggaaa gaaattagtt atgaacttag gtttctctca tccagttgag
     7981 atggaagatc cagaaggaat aactgttgaa gctccaaacc aaacagagtt aatagtaaaa
     8041 ggtatagaca agcaattagt agggaattat gctgctaaga taagagcttg gagaaagcca
     8101 gagccataca aaggtaaagg tataaaatac gttgatgaag taatcagacg taaagaagga
     8161 aaaactggta aaaaataata ccaaaactta aacgctagaa aggagtgagc taggtgttaa
     8221 aaaaggctga taaaaatgct aatagacttc aaagacataa gagagttcgt agaaaaataa
     8281 gcggtacttc tcaaagacca agattatgtg tatttagaag tgctaacaat atatatgctc
     8341 aaataataga tgacacaaaa agagtaactc tagttgctgc atcttcttta gaagcagaag
     8401 ttaagagtgc tgtcaatcat acaggaaaca aggaagcagc taagaaagtt ggagaattag
     8461 ttgctaaaaa agctgttgaa aaaggaatca ctgaagttgt atttgacaga ggtggatact
     8521 tatatcatgg aagaattcaa gaattagctg aaggtgctag agaagctggt cttaagttct
     8581 aatacgaagg agggaaaaat acatgctacg tcgtaagccg atagatgcag gacaacttga
     8641 cttacaagaa aaagttgttg aagtaagacg tgttactaag gttgtaaaag gtggtagaaa
     8701 tttcagattt gcagcattag tagttgttgg agatgaaaac ggacacgttg gtataggtgc
     8761 tgggaaagca atggaagtac cagacgctat aaaaaaagca gttgaagatg ctaagaaaaa
     8821 tctaatagta gtgccaatag ttgggactac aattcctcac gaagtacgcg gacactttgg
     8881 tgctggaaat atattaataa tgcctgctgt agaaggtaca ggagtaatag ctgggggacc
     8941 tgctagagcc gtacttgagc tagctggact aaaagacgtt agagctaaat ctttagggtc
     9001 taacaatcca agaaatatgg taaatgctac aatagaagga cttaattctc taaaaacagt
     9061 tgaagatata gctaaactta gaggtaaaaa agttgaagaa cttctagggt aaggaggtag
     9121 tgtaaaatgg ctaaattaca aataaagtta gttagaagtg ttataggaac tactcctaac
     9181 caaaaaaaga atgttgaagc gttaggatta agaaaaagag aacaagttgt tgtaaaagaa
     9241 gacaatgctc aaacaagagg tatgataaat aaagtaagtc acttattaga agtaactgaa
     9301 atagctgaat aatttttata aagtagtgca aggaggtgca atccatgaag ttacatgagt
     9361 taaaacctgc tgaaggtgca gtaagagcta agagaagatt aggtagaggt actgcaactg
     9421 gacaaggtaa aactgcagga cgtggacaaa aaggtcaatg gtctcgttct ggtggtggag
     9481 taagagtagg atttgaaggt ggacaaatgc cactagctag aagacttcct aagagaggtt
     9541 tcaataacat atttaagaaa gtttacacag aagttaatgt tgaagtttta aacagatttg
     9601 aaaatggaac agaaataact gcagaattat taaaatctac taaaactata agcaaaatag
     9661 gtaaagacgg aattaaaatc ttaggtgaag gtaatttaga gaaagcttta actgttaaag
     9721 ctgctaaatt tacagcttca gctcaagaaa aaatagaaaa agctggtgga aaagcagagt
     9781 tagtataata acctgctaat ctcctagcca gtacaggcgg ggtgaattag cgtgctgtca
     9841 aaattaaaac aagcttggaa gattaaagca gtaagaaaaa aagtaatgta tacacttatg
     9901 atgatcgtga tattcagaat aggtactact ataccagttc caggcataga cactagtatt
     9961 atacaaaaga tggtaggcgg caatagtcta ctttctctgt ataatatgtt tactggtgga
    10021 gcatttagta acttttcact atttgcttta ggtataagtc catatatcac tgcatcaatc
    10081 ataatacaac ttctaacagt aggttttgaa agtttagcag aacttcaaaa atctggtgaa
    10141 gaaggaaaga aaaaaatcaa taagtacact aaatatacag cactagctct agcagttgta
    10201 caagctttgg gaattacatt aggaattgta agaagtgctt taatatcaaa tagtgtgttt
    10261 tttatcacaa ctgttgtaat aacattagtt tcagcaagta tgttagtaat gtggattggg
    10321 gacaaaatta ctgaaaaagg aatcggtaat ggtagttcag taataatatt tgcaggtata
    10381 atatcaagaa tacctacaga tgttattaaa atatcgcaac aagttaaatc tggcgaagtt
    10441 gcaccttggg ttatagtgat tctagctgta gttatattac ttacagtaac aggagttaca
    10501 tttatacaag aagcaactag aaagatacct gtacaatatg ctaaaagagt tgttggaaga
    10561 aaaatgtatg gaggacaaag ttctcatata ccaatgaaag ttaaccagtc aggagttatg
    10621 cctataattt ttgctagttc acttttagct ttccctcaaa caatagctat gtttatgggt
    10681 ccaaatgctc aggcttttgt tcaaaaatac ttaagtatgg caacagaaca aggattttgg
    10741 acatatagat caattgagat tctacttata attttcttct catattttta tacaactgta
    10801 tcatttaata cagaagacat atctaaaaat atgaaaaata atggtggatt tataccaggt
    10861 ataagaccag gtgagcctac tatggattat ttaaatagaa ttttaactag attaacttta
    10921 gctggagcta ctttcttagc aataattgct atggtgccag ctcttacaac acattatatg
    10981 aaagtaaata tgagtctagc tgggacttca ttgctaatcg ttgttggagt tgctttggag
    11041 cttaagagac agttagagtc aaacttagtt atgagaagct atcaaggttt cttaaaataa
    11101 aatggagatg gataatatga gaataatatt acttggacct ccaggtgctg gtaaaggtac
    11161 tcaggcggca ggaatagtag aaaaatacaa tatacctcat atatcaacag gagatatatt
    11221 cagaaagaat ataaaagagg gaacagaact tggaaaaaaa gctaaagaat acatggacca
    11281 aggtttatta gtaccagatg agttaactgt aggtttagtt actgatagaa tatctcaaga
    11341 agattgtaaa aatggattta tgttagatgg atttccaaga aatgtagcac aaggagaaca
    11401 tttagatatc ttcttaaaaa atgctggtat atcactagat aaagttgtca atattgaagt
    11461 tgataagagt atattagtgt ctagagcagt tggtagaaga atatgtaagt cttgtggagc
    11521 tacttaccat gttgagttta atcctcctaa agtagaaggt gtatgtgatg tatgccaagg
    11581 agaattatat caaagagctg atgataatga agaaactgta tctaagagaa tacaagttta
    11641 tctagatgaa actaagcctt tagtagatta ttatagcaaa caaggtataa tagcagatat
    11701 aaaaggtgat caagcaatag ataaagtatt tgaagatatt gtcgcagcct taggaagtgg
    11761 aaaataaatg attatcttga aatcaaaaaa agaaatagaa cttttaagag aagcaggtaa
    11821 aatagttgct gacactcatg aagttttaag aaaagctatt tctccgggaa tatctacttt
    11881 agaattagat aaaatagctg aagagaatat aagaaaatac aatgcagaac cttcttttaa
    11941 aggatatgga ggttttccag gaagtatatg tgcttctatc aatagagagg tagtacatgg
    12001 tattccagga gaaactatat tacaagaagg tgacattgta agtttggata taggagccta
    12061 ttataaagga tatcatggtg attctgcgaa aactcacgga gtaggcatga tatctgaaga
    12121 agataggaaa ttgatagaag taactagaga aagcttctat gaaggaataa aatttgctaa
    12181 attaggatat agactttcag atatctcaca cgcagtacaa acacacgttg agaaacatgg
    12241 tttctcggta gttagagatt tagtgggtca tggagttgga gcaaatcttc atgaagaccc
    12301 acaagtacct aattatggac tcccaggaaa aggaccaaga cttagagaag gaatggttat
    12361 tgctatagag ccaatggtta atgcaggtcg ttatcatgta aagactctat cagatgggtg
    12421 gacgactgtt acaatagatg gaaaaaaatc agctcattat gagcatacga tagcaattac
    12481 tgaacatgaa cctttgatat tgacaaagtt gtaatttaaa aagtaggtga aaaaagtgct
    12541 atcagataat ttaagtatag gtcaagttgt taaagtttca ttaggacgag ataaaggaaa
    12601 tttattcttt gtagtcaaaa taattaataa tgaatatgta ttaatagctg atggaaaaaa
    12661 gagaaaactt gacaaaccta agttaaagaa agtgaagcac ttaaaaaaat ataattttat
    12721 taatgatgaa gttagaaaaa gagtagtatc tggacaagag attacagatt cttttttaag
    12781 agctgaactt actaagttaa attagataat ttaacttatt gaaatggagg gctggttagt
    12841 taatggccaa aaaagatgtt atagaattag aaggtacagt ttcagaagct ttacctaatg
    12901 ctatgttcaa agttaaacta gaaaatggac atgagatatt atgccacatt tctggaaagc
    12961 taagaatgaa cttcataaga attcttgaag gtgacaaggt gaatgttgaa ctttctccat
    13021 atgaccttac aagaggaaga attacttggc gtaagaagta gactatatta atatttagtc
    13081 taaggaggga ttaataatga aggttagacc atcagtaaaa ccaatatgtg aaaaatgtaa
    13141 agtaataaaa agaaaaggaa aagtaatggt tatctgtgaa aatccaaagc ataagcaaaa
    13201 gcaaggataa gaaattatta ctagcatttt gttaaatttt atagtataat actatattgt
    13261 gatgttaaca aaagaacgtt aaaatcgttg aacaatctgg cgtaggattg gcatatgatg
    13321 tgaagtttta aacactaaaa actatgtgta ggaggtgcga tcatggcaag aatagctggg
    13381 gtagatttac ctagagaaaa aagagcggag ataggcttaa cttatatata tggtataggt
    13441 aaagcaactg ctaacgaaat attagctaag gctgagataa atccagatac tagaataaaa
    13501 gatttatcag aagatcaagt taatgagtta agaaaagtta tagatgacga tttcttagtt
    13561 gaaggtgact taagaagaga aattgcttta aacataaaga gattaagaga tataaaatgt
    13621 tatagaggaa taagacacgc taaaggtctt ccattaagag gacaaagaac taagactaat
    13681 gctagaacta gaaaaggtcc tagaaaaaca gtatctcgta aaaagaaaaa ataaataaat
    13741 aataactaga tgaaggaggg aaaaggaaaa tggctaaacc aaaaaagaaa gttacacgta
    13801 ttagaagaag agaacgtaaa aatatagaac gtggtcatgc tcatatacaa tcaactttca
    13861 ataatacaat aataacttta actgacgttc acggaaatgc tatatcttgg gcaagttctg
    13921 gacaattagg attcaaagga tcaagaaaat caactccatt tgcatctcaa atggctgctg
    13981 aaacagctgc aaaagctgcg atggaacacg gactaaaaag tgttgaggta ttcgtaaagg
    14041 ggccaggttc aggaagagaa gctgcaataa gagctttaca agcaactgga ctagaagtaa
    14101 ctatgataaa agacgttact ccaatcccac ataacggatg cagaccacca aagagaagaa
    14161 gagtgtaatt aataagagta caataggagg tgtagataaa tggcaagata tacaggtgca
    14221 tcatgtagac aatgccgtag agagggaatg aaattattcc ttaaaggtga tagatgttat
    14281 acagacaaat gtgctatagt aaaaagaaac tatgctccag gtcaacatgg ccaaggaaga
    14341 aagaaagttt ctaactatgg attacaatta agagaaaaac aaaaagttaa aagaatatat
    14401 ggagttttag agactcaatt tagaaattta tatgaacgtg ctgaaaatat gcctggtaaa
    14461 gcaggtgaga acttattaag tttattagag agaagattag ataatgtagt ttacagaatg
    14521 ggactagcat cttctagaaa agaagctaga caattagtga ctcatggtca tttcacttta
    14581 aatggaaata aagtagacat accttcatta atagttaaag ttggagatgt tatagaagtt
    14641 aaagagaaat caagatcttc tgcaaaattc aaaaatttag tagaagttaa ctcaagaata
    14701 gctcctaagt ggttagaagc taatgtagaa ggaatgacag caaaagtagt tggtgttcca
    14761 acaagagaag atatagatct tgagatagca gagcacttaa tcatagaact ttactctaaa
    14821 taataagcac acttttaaaa attattttaa aattttgttt accctcagtg gattactgaa
    14881 ttttaaggag ggttttgtcc atgatagaaa tagaaaagcc aaaagtagat atagttgagc
    14941 ttagtgaaga ctatagatat ggtaagtttg ttatagaacc tctagaaaga ggatatggaa
    15001 taactatagg taacgcatta agaagaatat tattatcatc attaccaggt gtagcagtga
    15061 atgctataaa aatagatgga gttcttcatg aattttcaac aatacctggt gttaaagaag
    15121 atgttactga gataatatta actttaaaag agctttcagc aactatagat ggtgaaggaa
    15181 gtagaacact taaaatagaa gctcaaggac catgctctat cacaggtgca gatataatct
    15241 gtcctccaga tgttgaaata ttaagtaaag atttagcaat agctacatta gatgataatg
    15301 ctaaacttaa tatggagata tttgtagata aaggtagagg ttatgtttct gctgaagaaa
    15361 ataaaacaga gaatgttcca ataggtgttt tacctgtgga ttcaatatat actcctgttg
    15421 agaaggttag ttatcatgtg gaaaacacaa gagttggtca aaaaacagat tatgataaat
    15481 tagtactaga agtttggact aatggtagta taaatcctca agagggaata tcattagctg
    15541 caaaggtatt agttgagcat ttaaacttat ttatagactt aactgaacat gtaagtagtg
    15601 ttgaaatcat ggtagaaaaa gaagaagatc aaaaagaaaa agttcttgaa atgactatag
    15661 aagaattaga tttatcagtt agatcatata actgtttaaa gagagcggga attaatacag
    15721 ttgaagaatt agctaataaa tctgaggatg atatgatgaa ggttagaaac ctaggtaaga
    15781 agtcattaga agaggtaata caaaaactag aagaacttgg attgggatta aaaccaagtg
    15841 aagaatagcg agcaaaggag ggataagaat ggctaagtac cgtaaattag gacgtgaaac
    15901 agctcacaga aaccttatgt taagaaactt agtaacttgc ttactaagaa gtggaagaat
    15961 agaaactaca gtgactagag cgaaagaaac tcgtagaatg gctgaaaaga tgataactct
    16021 tgctaagaga ggagatcttc atgctagaag acaagtttta gcttatgtta tggatgaaac
    16081 agtagttaat aacttattca cagatttagc tccaaaatat gctgagagaa atggtggata
    16141 cactagaata ataaaaatag ggccaagaaa aggcgatgct gctgaaatgg cttttataga
    16201 attagtatag taaggaaagg attaggcttg atggcttaat ctttttttta tttttttgac
    16261 taaggtatga tataataatg tgtaaaatga tttttataag ttattgctta ctaaaaggag
    16321 tatgagttaa tggataatat agtaaaggta aataatattt cgtttgagta tattacagat
    16381 gaagcaaaac ttaaagcaat agataattta agcctagatg ttaaaaaagg agaatttgtt
    16441 gcgataatag gacataatgg ttctggtaag tctactttat ctaaaaactt aaatgctatt
    16501 cttatgccca ctgaaggaaa tattcttatt gatgatatgg atactaaaga agaagaaaga
    16561 ttatgggata taagacaaac tgcaggcatg gtatttcaaa atccagataa tcaaatagtt
    16621 gctactatag tagaagaaga cgttgcattt ggacctgaga acttaggtat agaaccaaaa
    16681 gaaataagac gaattgtaga ggagtcttta aaaagtgtag gtatgtatga tttaagagat
    16741 agacagcctc atttgttatc aggaggtcaa aagcaaagag ttgcaatagc aggaattatt
    16801 gctatgaggc ctaaatgtat aatctttgat gaagcaacag caatgttaga tccatctgga
    16861 agaaaagaag ttatgaaaac aataaaaaga cttaataagg aagaaaatat aactgttata
    16921 catataacgc actttatgga agaagcagta gaagctgata gagttgtagt tatggaaaaa
    16981 ggaaagaaaa tcttagaggg aactcctaga gaagtattca gtaaaataaa gatgttaaag
    17041 gaaatcggtt tagatgtacc ttgtatgaca gagttatcta gtttattaat agaagaaggt
    17101 ataaatatta gtagtgatat attaactgtg gatgagatgg tgatggaatt atgtcaatta
    17161 tagtaaagaa tttaacacat atatataatg aagggatgcc ttttgcaagt aaggcacttg
    17221 atgatgtttc ttttgagatt aaagatagag attttgttgg tcttataggt catacaggct
    17281 caggaaaatc tacattaata caacatttaa atggattact aaagccttcc tcaggagaaa
    17341 tatttataaa tgattttaat ataacagata aaaacttgaa tttaactgag attagaaaaa
    17401 gagtaggggt tgtatttcaa tatccagaat accaattgtt tgaagaaacg attgataaag
    17461 acatagcttt tggaccctct aatttaggct tagaggagtc tgagatacat aacagggtaa
    17521 aagcatcaat ggaagctgta ggacttgatt atgagggatt taaagacaaa tctccttttg
    17581 aattatctgg gggacaaaag cgtagagtag caattgctgg agttatagcc atgaacccag
    17641 aagttctcat attggatgaa ccaactgctg gtcttgaccc tggtggaaga gatgaaattt
    17701 ttaacttaat aaaagactta catgaaaaaa agaatatgac tataatacta tcatcacata
    17761 gtatggatga tatggcaaag ttagcaaaaa ctttaattgt tatgaatcat ggtagcgtag
    17821 aatttatggg aactcctaga gaagtattta aatcaaatgc tagtaaatta aaagatatag
    17881 gattagatat tccacaagtt ttagagcttg cattgaaatt gagagaaaaa ggctttgaca
    17941 tcagtgagga tatattgact ttagaagaag caaaacagga aatattaaaa gttgtgagag
    18001 gacgaggatt atgttaaaag atataactat agggcaatac tacccaacaa gttctgctat
    18061 tcataaatta gatccaagga taaagcttgt agcgacaatt gtatttatgg tatctatatt
    18121 tgtagtaaat aaattttggc cttatatagt agttttatta tgtttattag ctatgataaa
    18181 attggctaat ataccagtca aatatattgt taagggtgtt aaacctctta aatggattat
    18241 tttgtttaca tttcttataa atattttttt cttaccaggt gatgaaatat ggtcatttgg
    18301 atttttagct attacaaaac agggtctaag acaagcaatt tttatggcaa taagattaat
    18361 atttttagta gttggtacat cattgcttac tttaacaact tcaccaatag aattaactga
    18421 tggaatagag agattgttga atccatttaa gaaaattggt ttgccagttc atgaacttgc
    18481 aatgatgatg acaattgctt tacgttttat tcctacattg ttagatgaga cagacaaaat
    18541 aatgaaggct cagatgtcta gaggtgcaga ttttgaaagt aagaatctta taaatagagc
    18601 aaagaattta gtaccacttt tggtaccttt atttgtaagt gcatttagaa gagctgatga
    18661 attggctatg gccatggagg ccagatgtta cagaggtgga cataatagga ctaagatgag
    18721 agagtctgtt atatcaagaa gggattatat ggcatgtgta tttcaggttg tttatctggg
    18781 agcaattata gctactagat ttatagcaat ttaattaata agttatagat ataatgaggt
    18841 aaagtatgag aaatataaaa attaaaatcc aatacaatgg taagaattat tgtggatggc
    18901 aaaagcaacc tgattcttta gggatacaag gtacaattga aagggctata tatgatataa
    18961 caaaagaaga aaccagtctt atagggtctg gtagaactga ttcaggagtt catgctattg
    19021 gtcagattgc aaattttaaa attaattcag gtataagtat agaaagtata ccaatggcac
    19081 taaatgccaa gttgccaaaa gacatatcag ttatagaagc atgtgaagta aatgatgatt
    19141 ttcactccag atacagtgca aaaggtaaaa catataaata tttagtatat aatagcaagt
    19201 ttagaaatcc aatacttagt gagatatcat atcaagtaaa atatgaactt gattttgata
    19261 aaatgtgttc agaagcaaaa agtttattag gaacacatga ttttaaaggt tttatgagtt
    19321 ctggttcatc tgtaaaagat acagttagaa cgatatatga tattgatata agtaaaaaag
    19381 atgacttgat tacttttgag attagtggta atggtttttt atataacatg gtgaggatta
    19441 ttgttggaac acttgttgat atgggtcgtg gcagaattaa cgaaccattt ttagatataa
    19501 tacaatcaaa aactaggtca agatgtggac atacagctcc agcacaagga ttatttctca
    19561 aaaaagttca ttattaactt gacacaccag gctacatgta ttaaaatata tgagaatgtg
    19621 ttaataagtc cacttgcccc ggacttgaca taaacggtaa aaaaatttta tgaaaagtag
    19681 ttaaggaggg acagttatga aaagttatat agctaagcca gctgatgtac aaagaaaatg
    19741 gtacttagtt gatgctgaag gaaaaacatt aggtcgttta gcaacagaaa ttgcgacagt
    19801 attgagaggt aaacataaac caacatttac tcctcacgtt gatggtggag attttgttat
    19861 tgtagtaaat gcagaaaaaa tagttttaag tggtaagaaa ttagatcaaa aatattacag
    19921 ataccatact ggttatgtag gtggattaaa agaaatatct tacagagata tgatggataa
    19981 gaaaccagaa gaagttatat cacatgctgt aagcggtatg ttacctaaaa ataaattaag
    20041 aagcagaatg atgactagat taagagtatt tgcaggtgct gagcacactc atgcagctca
    20101 aaatccagaa gttttaaact ttaaataaga ttaataggtg aaaggaggag tttataatgg
    20161 ctaacgttca atattatgga actggaagaa gaaaaagttc tgttgctaga gtaagactag
    20221 ttgcaggtga aggaaatata ttagtaaatg gaagagcatt agaaaattat tttaattatg
    20281 agacattaat aagagatgtt aaacaaccat tagttttaac tggtaatgaa aataaatatg
    20341 atgttatagt aaaagttgaa ggtggaggat tcactggaca agctggagct ataagacatg
    20401 gaatatctag agctttatta aaagctgatt tagatttaag acctgcttta aagaaagaag
    20461 gtttcttaac tagagatgca agaatgaagg aaagaaagaa atacggatta aaagcagcaa
    20521 gaagagctcc acaattctca aaaagataat tacttatttt tttggaaaag caaaatcctt
    20581 tatttactgt tattcagaaa gtattggtat tttaaaaatg ttgggttaag ttaattattg
    20641 atatataata aaataataag atatcttatt aggatgtttt tcattaagaa gaacatcctt
    20701 tttattttga ctatttaata aaaaacatat gctaataaca tttaaatttt atttttataa
    20761 ta
//
LOCUS       DAEOSP010000052        20416 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.052, whole genome shotgun sequence.
ACCESSION   DAEOSP010000052 DAEOSP010000000
VERSION     DAEOSP010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20416
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.052"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            101..1132
                     /locus_tag="KRH54_001566"
     CDS             101..1132
                     /locus_tag="KRH54_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG8579233.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCL
                     DIEENNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            1265..2563
                     /locus_tag="KRH54_001567"
     CDS             1265..2563
                     /locus_tag="KRH54_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBG8579234.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(2677..2922)
                     /locus_tag="KRH54_001568"
     CDS             complement(2677..2922)
                     /locus_tag="KRH54_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBG8579235.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3070..3936
                     /locus_tag="KRH54_001569"
     CDS             3070..3936
                     /locus_tag="KRH54_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBG8579236.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(3940..4476)
                     /locus_tag="KRH54_001570"
     CDS             complement(3940..4476)
                     /locus_tag="KRH54_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBG8579237.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(4667..4870)
                     /locus_tag="KRH54_001571"
     CDS             complement(4667..4870)
                     /locus_tag="KRH54_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBG8579238.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            5161..5925
                     /locus_tag="KRH54_001572"
     CDS             5161..5925
                     /locus_tag="KRH54_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBG8579239.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKKTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            6314..7627
                     /gene="prdC"
                     /locus_tag="KRH54_001573"
     CDS             6314..7627
                     /gene="prdC"
                     /locus_tag="KRH54_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBG8579240.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      8030..8115
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            8697..10805
                     /locus_tag="KRH54_001574"
     CDS             8697..10805
                     /locus_tag="KRH54_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBG8579241.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPLDGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPS
                     DGQIPDGEKPSDGQMPDGEKPSDSQTPDVEKPSDGQMPDGEKPLDEQIPEEEKPLEEE
                     IVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEEDVKADEQSEINQVFTSEDSFISL
                     WYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTLTFDYVIKGLVGASSKDDKYVIE
                     GEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQENQNEEAVKSILYAGSEFDGFGY
                     KQQFNLGEEENDIMTYSATQSAIWIILGQIDEQERLKQYQGSIDLCDKLIEIAKTEEE
                     RAEYQKRKEVAINTKAYLEALLKAGREELKPNDTGKPSLSNGLTKINFEKNEDGTYET
                     EAVALVGYSGVVKLKLPDGVTAYDVDGNIIGTGEVEISTQQKFKLKSVGKPDAKANIS
                     AVSYDYIFPKAIQYYKAVLDLGQKDHSSSLPSSKQNLLSYTIEKKNGQEVNFNIGLPT
                     DDVVNPPVPPTDDTNGHKPKPSPPIDDTVINPPVPPMDDTIINPPVPPTEDAVLNPPV
                     PPTEDAVLNPPVPPTEDTVLNPPVPSVSDTVEKTPELSRDDTIIKSPKTGDETQLMSY
                     VFISVIAICGLAYQCKIKRN"
     gene            11212..12972
                     /gene="prdR"
                     /locus_tag="KRH54_001575"
     CDS             11212..12972
                     /gene="prdR"
                     /locus_tag="KRH54_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HBG8579242.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            13401..15281
                     /gene="prdA"
                     /locus_tag="KRH54_001576"
     CDS             13401..15281
                     /gene="prdA"
                     /locus_tag="KRH54_001576"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBG8579243.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            15362..15646
                     /locus_tag="KRH54_001577"
     CDS             15362..15646
                     /locus_tag="KRH54_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579244.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            15684..16409
                     /gene="prdB"
                     /locus_tag="KRH54_001578"
     CDS             15684..16409
                     /gene="prdB"
                     /locus_tag="KRH54_001578"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:16134..16136,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBG8579245.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            16489..17247
                     /gene="prdD"
                     /locus_tag="KRH54_001579"
     CDS             16489..17247
                     /gene="prdD"
                     /locus_tag="KRH54_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBG8579246.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            17262..17729
                     /locus_tag="KRH54_001580"
     CDS             17262..17729
                     /locus_tag="KRH54_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG8579247.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            17747..18229
                     /locus_tag="KRH54_001581"
     CDS             17747..18229
                     /locus_tag="KRH54_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBG8579248.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            18249..19256
                     /locus_tag="KRH54_001582"
     CDS             18249..19256
                     /locus_tag="KRH54_001582"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBG8579249.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            19376..20257
                     /locus_tag="KRH54_001583"
     CDS             19376..20257
                     /locus_tag="KRH54_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG8579250.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGAKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         8142 a         2375 c         3792 g         6107 t
ORIGIN      
        1 atgtttttag caagaaatat agcatttaaa agataaattg tgcaacttaa atcaaagttt
       61 aagatgaact attaacatag atatattaaa aacttttgta atggagaata tatgtgatat
      121 gaataataaa gttaaggttg cagtattaga tacaggaata gacaaagaac atgattactt
      181 aaaagataat ttagttggag gaattgcttt tgaatgtatg catgattata tctttatatc
      241 agataaattt gatgatgaag atggtcatgg aactgcttgt gcatcaataa taaagaaaga
      301 gtatgaagat gtagagttat ttgtaataaa aattttgaga aaagatggta ttacaaatat
      361 aaaagttctt gaagaagcat taagatacct tttagacact aacataagat taattaattt
      421 aagtttatca gtcataggag tagaaagtgt taagggctta tttgaagttt gttatgaatt
      481 gtttagaaaa ggaaaaataa tagtatgttc actcgctaat gattttgact taagttatcc
      541 agctatgttt aacaatgtta taggtgtaag aggatttact ttagatggag atgattcatt
      601 ttggtataat aaaaaatatg atgttcaatg tgttatggat agcaattcat atataagttg
      661 tgatataaat aattcctata gattaccacc aaaatgtaat agttatttat cagcaaagct
      721 tactggtaaa atagctaaga tattatcaga aaaaccaggc ataacaatat ctgatttaaa
      781 tgataagctt gaatcattag caactagaaa tcattgggat agttgtgatt ttgatgagtg
      841 tagtagaatt gtaaacttta aattggattt gtatgacaaa gaaaatgctc tttttatgaa
      901 agttgctgac gttgttaggg attgcttaga tattgaagag aataatgaaa aactctttaa
      961 gggtagcctt tttaataagg agataggctt ggtttatgat aattgtttta atctgttgaa
     1021 aaagctagag catagatttg acataaagtt taattatatg gacatctcaa aatatgactt
     1081 agtttcaata tatacactga ctgaattagt ggaaagatat aaaaacttat aaataaagat
     1141 ttgtagctaa atttaatgta gaaggtatta aattatatgt attctttgtc ggtagattaa
     1201 aagtaaaaaa tatactataa ttagatagca taagagaaaa tgacaataca gggggagtta
     1261 gataatgaaa tatgaagaag cattagagta catatcacaa actaataaat tcggtataag
     1321 attaggatta gaaaatatag gaaagttatt agaattatta ggaaatccac aagaaacttt
     1381 gaatataata catgtagctg gaactaatgg aaaaggttct gtgtgttctt ttgtttctaa
     1441 tatactaaga gagtgtggat ataaagttgg tttatataca tctccatatt tagaaacttt
     1501 tactgagcgt ataagagtaa atggacaaaa tataccacag gatgatgttg ctagaataat
     1561 tgagttaata aaagaaaaaa tagagattat ggtaaaagaa ggctatgcat atccgacaga
     1621 atttgaagtg gttactgcaa tggcatttta ttactattca gaacaaaagg tagactttgt
     1681 ggcattagaa gttggtttag gtggcagata tgatgctaca aatataataa caaaatcact
     1741 tgtaagtgtg atagtatcta taagtttgga ccatacaggt atacttggag atacaataga
     1801 aaaaatagcg tacgaaaagg ctggaataat aaaggaaaat ggagtagtac tagtatatga
     1861 ccagactgat gaagcaaagg atgttataaa gtcagtttgt aaagagaaaa aagctaaata
     1921 tatagaagtt gattttgatg atataaacat aaaaaaatca gatataaatt ctcaaatata
     1981 cgattgtact gtaatgaaag aaacttatag agaccttgaa ataaaattga taggcgaaca
     2041 tcaaataaac aattctatct tggcaattag tgtaataaaa tatctaaaag acataaataa
     2101 attagcaaat ataaatgaag aatctataag aaaaggactt ataaatacta aatggccagg
     2161 aagaatagaa aagataaaag aaaatccaat ttttataata gatggtgcac ataatgaaga
     2221 tggagcaaaa tctttggcaa aagcacttga taagaatttt aaaggtagaa aattaacctt
     2281 attaataggt atgttagaag ataaagatat tgatagtgtt ttagagatat tgttgccaca
     2341 ttttaataag gtaataacaa caacaccaaa taacccaagg gccataaatt cagatatatt
     2401 aagagaaaaa gtattaaaat atgtagatga tgtaacatca aagcatgaga tagaggatgc
     2461 agtaaattat acattagaaa catcaagtga ggatgatatt ataataagtg caggttcatt
     2521 atatatgatt ggtacagtga gaactttagt aaagaaatta taattttata aaataagtat
     2581 acaaataaaa atataaaaaa gagttgtcta aaaaatggac aactcttttt atataaaata
     2641 agtttttaat aattttactg atgtttatat aagtaattat atagattgac gtatagcttt
     2701 agataattgg tctgggcaag aagtaccttt tccaccacag tcaataccat ttaatttatc
     2761 agcaacttca agagcatttt ggccaattat taagcttcta agtcctgata aattaccatt
     2821 acatcctcct ataaactcag catcaacaat cacattattt tcatcaactt caaaaatcat
     2881 ttctctacaa catacaccac ttggattaaa tgttactttc attaaaaaga cctccattaa
     2941 atattatttc tttttttaac attaataata ataccaaata tatgcgtaat aatgcaacta
     3001 taaagcataa tacaagctaa tgaaataata tattattgta gtatatgtat tgtagagagg
     3061 aggggttata tgaaaatcta tatttctaac tacttatcaa agagtattag catagttgat
     3121 tattctacac ttgaactaga aaaagaaata gtattagaag ataatatata tccacaccat
     3181 ttttgcatag aaaaagaaaa aaatttaata tacataccta gttcaagtaa tgggatactc
     3241 tatgtcttag atttaagtaa tgataaaata attgatactg tctctatagg aggaagtctt
     3301 agtcaagttg tacttagcga taatgaattg tttgtggcaa atgaagattc taatagtata
     3361 tatgtactag ataaaaacac attaaatcca ataggaatta ttggagttga taatatgcca
     3421 catggatttg actttgatag agaaagcaaa aagttatatg taccgtgtat aaactcgata
     3481 gtatgtatag atacaattaa taaaaatata caaaagaaaa taaatatgga ctttagagct
     3541 tggcatatag aacttgataa acaaaagaaa gaaatgtata tatcaacact tgatggaaag
     3601 cttataataa tagatgaagt aagcatggat ataaaaaagg tattatatga atttttatta
     3661 ccagtagaaa taagatttaa ttatagtggc aaaaaagttt atgttgctga tttgggttat
     3721 aataatgtaa gaattttaga ttatacaacg agtagataca ttggaaatat tgaaataaat
     3781 gggattcccc aaggattaga aatatctaaa gatgaaaagt tactatttgt atcagataca
     3841 caagaaaatt cagtaaaagt ttatgaaact gcaaataaca aattaataaa agaaatcaag
     3901 gtaggaaaag agcctacaac tataatttgt ttgtaggctt taatgtatgt ctcttataag
     3961 taaatcgata acttcggcat acatataaga tgcattattt ttccagttat tgcaaataag
     4021 ttttgcttgt ttctctgatg aaacatttag atttaaatct attaagttag actgattttc
     4081 tatgactcta aggttgacaa taaattcatt atcactttgt tttacaaaac tagactttac
     4141 ttgagtatct gctagtagat tttctttgtt agaatctata tacttgtcta ttttttcagt
     4201 aacactagta ggtattcgat taaagaagta ttctagactt tctacgcctc tttgagtaag
     4261 agcataatat tctctgtcag agtctttata tgtagtcaaa aacttagatt ccataagctg
     4321 agataaaagc tgttgtaatg aaaaataatt catcatctca gtctctaata ctacttgagt
     4381 tatttgagaa ttagttaaat ccattttaat tttttctaat atatataaaa ttaaaagttt
     4441 gtgataggct aattcttcag acgagttttc aaacataaat atcatcctcc gtcaaactat
     4501 ttctatatat aatatattaa actaatttgt acaaaattaa aagaacaatt tgtattgtat
     4561 tattaatctt taataattca tataaaaaca attttataaa taaatataga gaaacagggc
     4621 tgctaattag cagccctgtt gatacccagg tgtcaaaatt tactatttat tttccagcca
     4681 tttgtctttc agccatttca actaatttct tagtcatata tccaccaaca tatccatttt
     4741 ctctagctgt taagttacct ttgtctatgt tttcatagtt agataaacct atttcattag
     4801 ctatttctaa tttcatttgg tttaaagcag cttttgcttc tggaactact gttctgtttg
     4861 aatttgacat agtaaatttc ctcctcaaat aattagttta tgatgtctaa caacactttt
     4921 atttatgtga tgttaatact aatttctcca aaataaaaat aatatataca gaaaaataaa
     4981 ggtaaaaatg taaaaaatat aaaaaaatta aataaatgag tattttagag agtttaatta
     5041 ggtcaatata ggttttattt ttttagttat tgtcttataa attaaatatt acagtacttt
     5101 tttgagctga aaaaaaataa attttaaata tagaagaaat tatttcaaag ggggaatagt
     5161 atgaaaagag aagttaaaag gactaaagct ttgatatata gtgttttatt tttttcatta
     5221 ttattagtgt taatatttgt ttttgtttat ggaataaaat acatatgttc taaatctgac
     5281 aattcaaaaa tgcctatata taaagttgat acaaatgaga aaaaaatagc tttgagtttt
     5341 gatgtagcat ggggaactga taatatggat gatattttaa aaattttaga taaacataat
     5401 gtaaaagcta ccttcttttt agttggtagt tgggtggatg ataatgaaga aattgtaaaa
     5461 gctatagatg aaaaaggaca tgaaattgga aatcattcaa acactcatgc caatttgaag
     5521 gaaatatcaa aagaagacat aacaaaagag atagaaacta catcagaaaa aatatataat
     5581 ataacaggaa aaaagactaa tttatttaga cctccttttg gggacgtaaa caataaagct
     5641 atggacattt gtagtgattt aggttataaa gtaattaagt gggatgtaga ttctatagac
     5701 tggaaggagc ttggaccaaa tcatgtaatt gaaaaagtaa taaaagaatc tcaacctggt
     5761 tcaatagttt tattccatgc taatataaat gatgtagata actatttgga tactataata
     5821 agtcgactaa aaaaagatgg atacagtcta gtcaaaatat ctgatttgtt gtataaagat
     5881 aattatgtag ttgattccaa tggagtgcaa aaaactaaga attaataagt aataaatgta
     5941 tattttaagt aaagttgtga taaattatat agtatatgtt agaaaagtgt cgataaaaag
     6001 acggtgtcta ttttttgaca ctttttatac atgtatgcaa tatcaataat tatagattaa
     6061 attgattaaa aaagtgtcta ataattgact tttttttgat gggtttaaaa atttagaaga
     6121 attaatgaat gctgtaagac agtatttcca aagggtataa gtttgtttaa aagttggcat
     6181 agaaattgct ttgttaataa gcgatatgtt ctatagaaat aaacaatatg ttaagacctt
     6241 ataatagaaa aatagcatta acattatttg aacagcttat aaaaaaatta atatacttaa
     6301 gggggcgtac tagttgaaca aactatattc tattttatta aaacaacacg ttggtggtcc
     6361 agataagcca gtagtaagtg ttggagatgt agtaaaaaaa ggaactctaa tcgcagaacc
     6421 aactggatta ggggcaaata tttatgccag tgttagtgga aaaataagtg aaataaatga
     6481 ccaagcgata gtaattgaag ctgatgaagt acaagaagat acttttgaac cattaaaagg
     6541 agaaggtata cttgacttaa ttaaagaagc tggagtagta ggaatgggtg gagcaggatt
     6601 ccctactcat gtaaaattaa acatagattt aaatggtgga acaatattag caaatgcagc
     6661 tgaatgtgaa cctttattag ctcataatat aaaagaaata gaagaaagac cagaaatagt
     6721 ttatcaaggt ataaaatatg ctatggaagt tactaatgct ggaaaaggta tgctagcaat
     6781 aaaaagtaag catccaaaag caatagaagc tttcaaaaaa gtaataaagc caggagacaa
     6841 catagaagtt gcagaattag ttgatatgta cccaatggga gaagagagag caatagtaag
     6901 agacgttcta ggaaaattac ttgagccaac tcaattacct tcagaggcta atgcagtagt
     6961 aataaacgta gaaacactaa ctagaatagt agaggctgtt gagcaaaaaa gaccagttat
     7021 atctaaaaat ataactgtag ttggtcaatt aaacagtgga aaagaatcta tagtgtttga
     7081 agatgtgcca attggaacaa ctgtcggaga attaattgaa agagctggtg gaataaaagg
     7141 tgaatatggt gaaataatcc taggaggacc tttcactggt aaagctacta ctttagatgc
     7201 tccaataact aaaactagtg gtggtataat agttactatg ccatttgtta atgaaaagag
     7261 aaaaatgggt cttttagttt gtgcatgtgg acctaatgaa gaaagaatga gagatatagc
     7321 aactaaaatg ggagttactg atatagtatc agttcaaaaa tgtaaacaag ctcaagaaat
     7381 taaaggtgca ttaaaatgtg aaaatccagg acactgtcca ggacaagctc aaaaatgtat
     7441 tgaatttaag aaagctggag cagaagttat attaatagga aactgtactg actgtagtaa
     7501 cacagtaatg ggttctgcac caaaattaaa attaggtgta tatcatatta ctgaccatgt
     7561 aatgagaaca gttaatcacc cattaataag aagaataaaa ggtgacgtaa gattaaaaat
     7621 aaaataatag ataaatagaa gttagacatc tttagaagta aaacttaaaa gttacttcta
     7681 aagatgtttt ttaatgtatt ttgagggcaa tatatattgt aaaataaatg gaaaaaatag
     7741 acaaaatatg ctagatattg tataatgata acaatagcta ttacaattaa tattggacaa
     7801 aaataatgat actcaagtat gtggtttgta ttaatatgca tattatatta agtcaactat
     7861 ccgatagagt aaaaggtttt tgtgatattt gaagatactc tatagtatct tttttattat
     7921 aaaaactaat ttttgtcttt tattagataa gtatctagga tactaattta tattttattg
     7981 agttgttaaa aaagtgcact taaaaagtta aaaaagaaaa attaaaaaac ctatttatag
     8041 aaactgtgaa gtatatctta aacctgggca cttaaaagat atatggagtt agtagtgcaa
     8101 cctgctataa atataaatag gagtaacttt taattgtcaa aaatatataa aataaataca
     8161 taaataagta attaatagaa gatagatata aatatatttt tgaaatgctc cctgcattca
     8221 taacgagtgt agagtggcag atatctgccc aaaactcctt taattgctgg aagttcctaa
     8281 agctaactaa accacaacat aatcatgaaa taaggataag tgtgatagag tgtgcattta
     8341 ataagcactt gaggttacga aagtagaaaa aattagttag atagcataag gttaaatcct
     8401 aagtgccttg aaaatggata atcagcaggt aagcttcgaa aagaagaaac ttcaacgact
     8461 agagagaaat ctcgtaccta caagtgtggg gaaatgggga gtacctaatt tatggttagg
     8521 taatgatata gtctgtgctt aatagaaata ttaagaagtt cataggagaa ctggttagga
     8581 gtaacgaccc taattgaaca cactaaagat gtattattga ataagattca tataatattt
     8641 aataatttta attatattaa gtgaatacaa tatatgtttt tagtatcagg agaattttga
     8701 aaacaaaaat taaaaaatca agtataatca gtttagtaat tgcttttagt atggtattta
     8761 ctgcatttac accaatagta tcttatgctg acgaagtgac tagcaatgat acaatactta
     8821 atggagaaga acaatcaaat ggtcagacac cagatgtaga gaaaccatca gacggtcagg
     8881 taccagatgg ggagaaacca ttagatggtc agataccaga tggagagaaa ccatcagatg
     8941 gtcagatacc agatggagag aaaccatcag atggtcagat accagatggg gagaaaccat
     9001 cagatggtca gataccagat ggggagaaac catcagatgg tcagatgcca gatggagaga
     9061 agccatcaga tagtcagacg ccagatgtag agaaaccatc agatggtcag atgccagatg
     9121 gggagaaacc attagatgag caaattccag aagaagaaaa accattggaa gaagaaatag
     9181 ttatagagga aatgagttta aaacaagata ttgataaaat tcttgatatg actttatctc
     9241 aaataaataa aatagtatat aacttttggg aagatgaaga agatgtaaag gcagatgaac
     9301 aatcagaaat taaccaagtt tttacttcag aagactcatt tattagctta tggtatgaca
     9361 aaaaagcaaa ggtgaaaaat tcttgtttac taaaagaaat agatggttca gatagacctt
     9421 atcatgattt aagatttgat gatattacaa agactttaac ttttgactat gtaataaagg
     9481 gtcttgtagg agcaagctca aaagacgaca aatatgtaat agaaggtgaa gatggagagc
     9541 aaactgcatt ttgttacaat aatcatttaa gaccaccaag tagtaatgga aagagtccat
     9601 atttaccagc agaagatttt aatggtcaag aaaaccaaaa tgaagaagca gttaaatcta
     9661 tactatatgc aggttctgaa tttgatggat ttggatataa gcaacaattt aatttaggcg
     9721 aagaagaaaa tgacataatg acttactcag ctactcaaag tgcaatttgg attattcttg
     9781 gacaaataga tgaacaagaa agacttaagc agtatcaagg ctctatagac ctttgtgata
     9841 aacttataga aatagcaaaa actgaagagg aaagagctga gtatcagaag aggaaagaag
     9901 tagcgataaa tacaaaagct tatttagaag cattgttaaa agcaggtcgt gaagagttaa
     9961 agccaaatga tacaggaaaa cctagtttga gtaatggttt aactaagatt aactttgaaa
    10021 aaaatgaaga tggtacttat gaaacagaag ctgtagcctt agtaggttat agtggtgtag
    10081 taaagttaaa actaccagat ggagtaactg cttatgatgt agatggtaat ataataggta
    10141 caggcgaagt agaaatttca acgcaacaga aatttaaact taagagtgtt ggaaaaccag
    10201 atgccaaagc taatatttct gctgtatctt atgattatat ttttccaaaa gctattcaat
    10261 attataaggc agtgcttgat ttaggacaaa aagaccattc atcttcatta ccatctagta
    10321 agcaaaattt attaagttat acaatagaga aaaaaaatgg tcaagaagtt aactttaata
    10381 taggtctacc aacagatgat gttgtaaatc caccagtgcc accaacagat gatacaaatg
    10441 gacataagcc aaaaccatca ccaccaatag atgatacagt aataaatcca ccagtaccac
    10501 caatggatga tacaataata aatccaccag taccaccaac ggaggatgca gtgttaaatc
    10561 caccagtgcc accaacggag gatgcagtgc taaatccacc agtgccacca acagaggata
    10621 cagtgttaaa tccaccagta ccatcagtaa gtgatacagt ggaaaaaact ccagaactat
    10681 caagagatga tacaataata aaatcaccaa aaacaggtga tgaaactcag ctaatgtcat
    10741 atgtatttat tagtgtcatt gcaatttgtg gattagcgta tcaatgtaaa ataaaaagaa
    10801 attaatctag attattatta agtttaaaat aaaaagtctc aaattgattt ataagtcatt
    10861 ttggggcttt tttatgtaaa tataatatgc aaatatagtt tgtttaaaaa tatatatttt
    10921 aattatattc ttaaatttat gattggatac aggcattcat taattgtttg atgaatagaa
    10981 aaatgttttg aaggtgaaaa tagtattgaa aaaataatat actaagggta taattcattt
    11041 gatttcattt gtataaattt aattataaat tttaaagtga ttatataaat ttaaatgcta
    11101 attacttaat aaattttaat aataattatt taataaataa ttattataat atgctagaat
    11161 aaatatatat aaataagttt tgatgaggta ctactataaa ggggggactt tatgttttcg
    11221 ataccagaag taaaaaaagt agaagaggta atggatacaa aatttacaac tatagacgaa
    11281 gacacaagaa ttgaagatgc aataaaagaa atgataaaaa gcaacacaaa aactctaatg
    11341 gtaatagatt catctgacca attaaaaggc attatatcca tgacggatat acataatctt
    11401 tatgagatgc ataaaaagta tgaaggtcaa ccagtaaaac ttataatgaa gaaggatgta
    11461 atatatgtca acgaaggttt aaccttagat gaatgtagag atataatgat attaaaaaat
    11521 atagggattc ttcctgttct tagagataat aaaattattg gagttctaaa acaagagcac
    11581 attagggact atctatatat gcatttagaa gattatggat taactttgaa gtatattata
    11641 ggtcaaatta aagaaggaat ttgtgctata aataacgaag gtgttgttat cttgtggaat
    11701 aagtttatgg aggaaaggta tgatataaag tctgaagata tagttggtcg accgatgaat
    11761 gaatttttgg aaaatactat ttcagaaaaa gtactaaata gtaaagttgg aatgagtgac
    11821 ttgtatttta cagataaaaa agaaaatatg tatgcattag ttcatgcaaa tcctatattt
    11881 tataaagaag aatttatcgg tgttgtatgt acagaagttg atgttacaga agctaagata
    11941 ttagctcttg aacttgaaaa agtaaatgat acattaaaat atctaaagaa cgaagtcaaa
    12001 aatttatcta aaggtagttt tgataagatt ttaggtaaaa gttataaatt ggaaaaatcg
    12061 aaagcgattg caaaacaagt ggctagaaca aatagtagca tatttatttg gggcgaaagt
    12121 ggtacaggta aagaagtatt tgcaagagct attcatgatt atagtgagag aaaaggtcaa
    12181 tttatacctg ttaactgtag tgccatacca aatgagttat ttgaaagtga gttttttggg
    12241 tatgaatcag gtgcgtttac aggagcaagt aaaaagggtc gaataggtat atttgaactt
    12301 gcaaaagatg gaactgtatt tttagatgaa attgccgatt tacctcttag tatgcaagca
    12361 aagttgctta gagtattaca ggaaaaagaa ataagaagag ttggtggaga taccacaata
    12421 aaaataaatc ctagaataat atcagctacc aataaagatt tgagtaaaat ggtaaaggca
    12481 gaaaaattta gagaagattt atactataga ttaaatgttg ttgaaataaa gattcctcca
    12541 cttagagaga gaaaagaaga cataggatta ttggttcata gctttttaga ggagatatgt
    12601 aaacaaaata ataagcctgt tcttacaatt agcaaagatg taatagatat atttcagaat
    12661 tatagatgga aaggtaatat tagagaatta aaaaatacga ttgaaaatat tgttgtattg
    12721 tcacaaaatt ctaaaataga tgtggatgat gtaccaagtt atatgatgga ttctacaaat
    12781 aatagtactg aagaagaaga gtatccttta gatttaacga aggcaactca aaaaattgaa
    12841 ataaagaata taaccaaggc acttaaaatg tctaatggaa ataaagctaa agcagctaag
    12901 atattaaata tacccagaac aactttgtat tataagatag accaatacaa aatagatgtg
    12961 tctaaaatat gacacatgac taaattttga caaaatgata aactttaatt taaaacctag
    13021 taaagccaac gcttatataa gtgttgtgta tatagatgtg ttaaaaaaaa tgaaaaagtg
    13081 ttgattaatt gacacttttt ttgtttgaga aaatgtttac aaatacaaaa aataacatat
    13141 tttaaataaa tatttatata atatttggct aaaaatagcg atagatgaag aatctattat
    13201 agaaaaaatc tttttgttaa taagttggca taggaattgc ttataatata aagtgacttt
    13261 aagtgagaaa aaaataatat taatcataac aaattatgta ggttttaatt tacttaattt
    13321 ctcagttaag gggttacctg ttgaggaatt gagatttaaa ataataaata agaaaataat
    13381 gtaaggaggt attttatact atgtcaataa ctttagaaac agctcaagcc catgcaaatg
    13441 acccagcagt ttgttgttgt agatttgaag cgggaacaat tatagcgcca gaaaacttag
    13501 aagatccagc aatatttgca gacttagagg attctggatt attaacaata ccagaaaatg
    13561 gattaactat aggtcaagta ctaggagcta agttaaaaga aactttagat gcactttctc
    13621 caatgactac agataacgta gaaggataca aagcaggaga ggctaaagaa gaagtagtag
    13681 aagaaacagt agaagaagca gctccagtat cagaagcagc agtagttcca gtaagcacag
    13741 gagttgcagg tgaaacagtt aaaatacaca taggtgaagg taagaacata agcttagaga
    13801 tacctttatc agtagctggt caagcaggag ttgctgctcc agtagcaaac gttgctgctc
    13861 cagtggcaag tgcagcagca gaagtagctc caaaagttga agaaaagaaa cttttaagaa
    13921 gcttaactaa aaaacacttt aaaatagata aagttgaatt tgctgatgaa actaaaatag
    13981 aaggaactac tttatacatc agaaacgcag aagaaatatg taaagaagct aatgaaactc
    14041 aagagttagt tgtagatatg aagttagaaa taataactcc tgataaatat gaaacttaca
    14101 gtgaagctgt attagatata caaccaatcg ctactaaaga agaaggcgaa ttaggttcag
    14161 gtataactag agttatagat ggagctgtaa tggtattaac tggtacagat gaagatggag
    14221 ttcaaatagg tgaatttggt tcttcagaag gtgagttaaa tactactata atgtggggta
    14281 gaccaggtgc tgctgacaaa ggtgaaatat tcatcaaagg tcaagtaaca ataaaagcag
    14341 gaactaacat ggaaagacca ggacctttag ctgctcaccg tgcatttgac tatgtaactc
    14401 aagaaataag agaagcatta aagaaagttg acaactcttt agtagttgat gaagaagtaa
    14461 tagagcaata cagaagagaa ggtaaaaaga aagttgttgt tataaaagaa ataatgggac
    14521 aaggtgcaat gcatgataac ctaatattac cagttgagcc agttggtaca ttaggagctc
    14581 aaccaaacgt tgacttagga aacatgccag ttgtattatc tccacttgaa gtattagatg
    14641 gtggtatcca tgcattaact tgtataggac ctgcatcaaa agaaatgtca agacattact
    14701 ggagagagcc attagtaata agagctatgg aagacgaaga aatagattta gtaggtgttg
    14761 tatttgttgg ttctccacaa gtaaatgctg agaaattcta tgtatctaag agattaggta
    14821 tgttagttga agctatggaa gttgatggag ctgtagtaac tactgaaggt ttcggaaaca
    14881 accatataga tttcgcatct cacatagagc aaataggtat gagaggtata ccagtagttg
    14941 gtgtaagttt ctcagctgtt caaggtgctc tagttgttgg taataaatac atgactcaca
    15001 tggtagacaa caataagtct aagcaaggta tagagaatga aatattatct aacaacactt
    15061 tagctccaga agatgctgtt agaataatgg ctatgcttaa aaatgctata gaaggtgtag
    15121 aagttaaagc tcctgaaaga aaatggaatc caaatgttaa attaaataac atagaagcta
    15181 tagaaaaagt tacaggagaa aaaatagtat tagaagagaa tgagcaatct ctaccaatga
    15241 gtaagaagag aagagaaata tacgaaaaag acgaaaacta aaatttagat actaactaag
    15301 atttagacaa taacataaat ttagatccaa gtttaaaaca gtatagagag gtagaattat
    15361 aatggatagg ttaaaatata tctcatcaga gactttttat gaaggtatta tagttgacat
    15421 caaaggcggt ggagtgacta tagacctaaa aggtagactt ggacaattta aaatacctaa
    15481 tagaatgctt ataactgact acgaacttaa gataggtcaa gaagttggat tcatgctaag
    15541 ctatcctgag gtattaagtc cagagcctaa tcaagagtat gtagaaaata ttaggagaga
    15601 aaaagaaaaa caagcagaaa tgcaatcaaa acaagaagaa aaataataaa taatcgtttg
    15661 aaaatagaag ggagaggaaa tatatgagcc ttacaacagt acaaggactt caatctgaaa
    15721 tattcgttcc aataacacct cctccagttt ggactcctgt aactaaagaa ttaaaagata
    15781 tgactatagc tttagctaca gcagctggtg ttcatttaaa atctgacaag agatttaatc
    15841 tagcaggaga tactacattt agagctatac ctaatacagc tacagttgat gaaatgatgg
    15901 tatcacatgg tggatatgac aatggagatg taaacaaaga tataaactgt atgttcccta
    15961 tagatagatt acatgaatta gctgcagaag gatttatcaa aggagtagct cctatgcact
    16021 atgcattcat gggtggtgga ggaaaccaac atgtcttcac tgaagaaact ggtcctgcta
    16081 tcgctgctaa acttaaggaa gagggagtag acggtgtagt tatgacagct ggctgaggta
    16141 cttgccatag aactgccgtg atcgtgcaga gagcaataga ggaagctgga atacctacaa
    16201 taataatagc agctcttcct ccagtagtta gacaaaacgg aactcctaga gcagttgctc
    16261 cattagttcc aatgggtgct aatgctggtg aaccacataa tatagaaatg caaacacata
    16321 tattaagaga tacattagag caattagttg caataccatc tgctggtaag atagttccat
    16381 taccatacga atataaagct cacgtttaaa atgtaattta tatttgaaaa tatagaaccc
    16441 ttctcctaca cggttaggag aggggtttta agaacaaagg tggatattat ggaagaaaaa
    16501 ataatgagaa gacttgttat aaaaccgttt catatgaatg aagttaattt cggttcaaaa
    16561 acttctataa agaaagatgt acttacaata gatttatcaa gtatagatga aataaaagaa
    16621 agagaagagt taataacaga cataaaagta gacattataa aacctggaga ttatgacaga
    16681 gaaattaata ctattatgga cattatacca atatcaacaa aagttttagg cagactggga
    16741 gagggtatta cacatacttt aacgggtgta tatgtaatgt taactggtgc agatgaagat
    16801 ggaaatcaaa tgcatgaatt tggttcttca gaaggaattt tgaaagacca aatggtattt
    16861 gggagatatg gaactccatc agttgaagat tatataatcc atgtagatgt aactttaaag
    16921 ggaggtttac cttttgaaag aacacttcca cttgcagcat tcagagcatg tgatgatttt
    16981 atacaagaaa taagagcgtc tttgaaaatg gaagacggaa gaaatgctac tcaagttcgt
    17041 gaatattttg acaaaattcg accaaatgca aaaaaggttg tcatagtaaa acaaatagca
    17101 ggtcaaggtg ctatgtatga caatcagtta ttctcaaagg aacctagtgg atttgaagga
    17161 ggaacatcaa ttatagatat gggaaatgta cctatgatta tatctcctaa tgaatacaga
    17221 gatggtgccc ttagagctat gacttaagag aggtggatat tatgggaata ggaccttcaa
    17281 caaaagaaac atctcttcat cactttagag acccactttt agacatagtt gaaagtgata
    17341 aggatgtaga tttacttgga gtaatagtag taggaactcc agatggaaat gaaaataaaa
    17401 cctttgtggg acaaagaacg gccgcttggc tggaagccat gagagtagat ggagctatag
    17461 tttcatcaga tggttgggga aactcacatg ttgactacgc taacacattt gaggaaatag
    17521 gaaaaagaga tatacctgta gttggagtta catttaatgg gactcaagct aagtttgttg
    17581 taagcaatca atatatggat accatagttg atatgaacaa atcaaaagaa ggtattgaga
    17641 cagaagtagt tggagaaaac aacacaaatg aaatagatgc taaaaaagct ttggcatttt
    17701 taaaacttaa aatgagaaaa catggataaa aaatttaagt aagggaatga gttttgtggg
    17761 aatttctaca tcaatgaaaa acacatcact tcatatattt agagacccgt tactagattt
    17821 gatagataat gattctgaca tagacttgtt aggagtggtt gtagttggga catcagagca
    17881 caatgaatgg aaaacatttt tagcaactag acttggaaga tggattgaat cattgcgacc
    17941 tgatggagtg attataactt tagatatggc aggaaaccag catattgatt ttacaaatgc
    18001 tatagcagag tttgtaaaat cagatatacc tacagtaggt cttactatta tgggagcaga
    18061 tggattagta attaccaatc catatttgga taaagctacc ataattgatt ataaaaagac
    18121 aacaggctac atagaaacag aagtagttgg agacaatcac atggatgaag ttgatgttaa
    18181 aaaagcattg gctttcctaa aattaaaaat gcgtaaagac gcaaaataaa cctaaggggg
    18241 aatttgatat gaaatttagc agaagtatac aagctataga ctctcataca gcaggtgaag
    18301 cgactagaat agttgtaggt ggaataccta acataaaagg aaattctatg cctgagaaaa
    18361 aagaatattt agaggaaaat ctagattatt taagaactgc tataatgtta gagccaagag
    18421 gacataatga tatgtttggt tctgtaatga ctcaaccttg ttgcccagat gctgactttg
    18481 gaataatctt catggatggt ggcggatacc ttaacatgtg tggccatggt acaataggag
    18541 ctatgacagc agctatagaa acaggtgtag ttccagcagt agagcctgta actcatgtag
    18601 ttatggaagc tccagcagga ataataagag gagacgttac agttgtagat ggaaaagcta
    18661 aagaagtatc attcttaaac gtaccagctt tcttatataa agaaggtgtt gaagttgact
    18721 taccaggtgt tggaactgtt aaatttgaca tatcatttgg tggaagcttc tttgctataa
    18781 tacatgcaag tcaattaggt ttaaaaatcg aacctcaaaa tgctggtaaa ttaactgaac
    18841 tagctatgaa acttagagat ataataaatg agaaaataga aatacaacat ccaactttag
    18901 ctcatataaa aactgtagac ttagttgaaa tatatgatga gccaactcat ccagaagcta
    18961 cttacaaaaa tgtagttata tttggtcaag gtcaagttga cagatctcca tgtggaactg
    19021 gaacaagtgc taaattagct actttacatg ctaaaggtga attaaaagta ggagaaaaat
    19081 tcgtatatga aagtatatta ggaactttat tcaaaggtga aatagttgaa gaaactaaag
    19141 ttgcagattt caatgctgta gtacctaaaa taactggttc tgcttatata actggattta
    19201 atcattttgt aatagatgaa gaagacccac ttaaacatgg atttattctt aaataagtaa
    19261 taaataaatt aggaaattaa aatagaatag tagccacttg tcggttgcta ttctatagct
    19321 tttaggctat aagattcaga atatatgaaa aaataaaaaa ataggaggat ggattatggt
    19381 agcaatctta aaggcattat atgtagtatt cgcagcaatc tttgcagttg tgtttggtaa
    19441 agacgtagct aaagcaaaca aagaggggaa actagaagaa caaagtactc caaaagttgc
    19501 tgtaacagga tttataacag acttctttga tacattagga atagggtcat ttgctccaac
    19561 aatagcaatg tcaaaggctt taaagttaaa tataccagat aaaaaaatgc ctggtacatt
    19621 aaacgtagca catactattc cagttgttac agaagcattc atatttacat caattatccc
    19681 agtagatggt gttactttag tatctatggt tgtggcagca gcagttggtt catatatagg
    19741 tgcaggaata atagctaaga tggacgagag aaaaatacaa cttgttatgg gtgtaacttt
    19801 agcattaaca gcaatattaa tgttattagg tcatccttgg attaacgtat taccaggtgg
    19861 aggaaatgaa ttaggtctta ctggtgctaa attagtaata ggtgtaatag gtaactttat
    19921 actaggagct ttaatgacag ctggtatagg tctatatgct ccaggtatgg caatggttta
    19981 tttcttagga atgtctgcaa aagttgcatt cccaatagtt atgggttctt gtgcattatt
    20041 aatgccagtt gcaagtatga aattcataaa agaagatgca tatactaaaa aagcatcagt
    20101 tataatagct atatgtggtt taataggagt tttcgtagct gcatatatag taaaaactct
    20161 accaatggat atattaaaag cattagtaat agtagttata gcttatactt ctgctacaat
    20221 gataatggct gcaaataaaa acaaaaagac tgcttagtta aagactactt agtgtttatt
    20281 aaatattaca gctaataata aagattataa actgctttaa aatattgaat taatatttta
    20341 gggcagtttt ttatttacta aattaatata aagcaattaa catgccaatt atgtaatata
    20401 tatagtgatt tagtag
//
LOCUS       DAEOSP010000053        20286 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.053, whole genome shotgun sequence.
ACCESSION   DAEOSP010000053 DAEOSP010000000
VERSION     DAEOSP010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.053"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(63..1268)
                     /locus_tag="KRH54_001584"
     CDS             complement(63..1268)
                     /locus_tag="KRH54_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBG8579251.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1443)
                     /locus_tag="KRH54_001585"
     CDS             complement(1291..1443)
                     /locus_tag="KRH54_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG8579252.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            complement(1446..2831)
                     /locus_tag="KRH54_001586"
     CDS             complement(1446..2831)
                     /locus_tag="KRH54_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBG8579253.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2860..3246)
                     /locus_tag="KRH54_001587"
     CDS             complement(2860..3246)
                     /locus_tag="KRH54_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG8579254.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3262..3576)
                     /locus_tag="KRH54_001588"
     CDS             complement(3262..3576)
                     /locus_tag="KRH54_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG8579255.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            4278..5090
                     /locus_tag="KRH54_001589"
     CDS             4278..5090
                     /locus_tag="KRH54_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG8579256.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPANYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            6138..6899
                     /locus_tag="KRH54_001590"
     CDS             6138..6899
                     /locus_tag="KRH54_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG8579257.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLSRILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            6964..7746
                     /locus_tag="KRH54_001591"
     CDS             6964..7746
                     /locus_tag="KRH54_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG8579258.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            8013..8432
                     /locus_tag="KRH54_001592"
     CDS             8013..8432
                     /locus_tag="KRH54_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBG8579259.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(8535..8801)
                     /locus_tag="KRH54_001593"
     CDS             complement(8535..8801)
                     /locus_tag="KRH54_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBG8579260.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            9028..9729
                     /locus_tag="KRH54_001594"
     CDS             9028..9729
                     /locus_tag="KRH54_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579261.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            9811..10419
                     /locus_tag="KRH54_001595"
     CDS             9811..10419
                     /locus_tag="KRH54_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG8579262.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            10743..11207
                     /locus_tag="KRH54_001596"
     CDS             10743..11207
                     /locus_tag="KRH54_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 domain-containing lipoprotein"
                     /protein_id="HBG8579263.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            11844..12860
                     /gene="gap"
                     /locus_tag="KRH54_001597"
     CDS             11844..12860
                     /gene="gap"
                     /locus_tag="KRH54_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG8579264.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            13255..14424
                     /locus_tag="KRH54_001598"
     CDS             13255..14424
                     /locus_tag="KRH54_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579265.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            14804..19057
                     /locus_tag="KRH54_001599"
     CDS             14804..19057
                     /locus_tag="KRH54_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBG8579266.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDIPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            19206..19700
                     /locus_tag="KRH54_001600"
     CDS             19206..19700
                     /locus_tag="KRH54_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG8579267.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            19816..20193
                     /locus_tag="KRH54_001601"
     CDS             19816..20193
                     /locus_tag="KRH54_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBG8579268.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
BASE COUNT         7734 a         2426 c         3572 g         6554 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacg gggggttaag tgctggcgtg gctattgcca caccagccag
     1441 caagatcagt ccacacctgc gacttccgct tcgcacgtcg cctgcgtgga ctgtctgctg
     1501 ttggataact ttttaatttc ctccaagaaa tcatatcctt ttggtacaag gggagtataa
     1561 aactctgata tgacacttgt tcctacatca acatagccac gacctttgat tcgctttaag
     1621 aagaaatcct tttgtacgtc actgccaaac atcatgccat agcccatttc agacatacga
     1681 cctaaagcca ctctgaaatt aaactgatca cggattccgt cgcctaaata ttttgcgtct
     1741 ggacgttgac aagccagtat tagaaagaag ccagcttgac gacctaacat gacaatctgt
     1801 ttcagcttat tcataactgc ggtgttttct tttgttccca gcatttccat gaaagcgacg
     1861 tattcatcaa agattaagaa gtgtgccggg agacctaagt aagcataatt tttgccagtc
     1921 ttatagttct tcatctgctt catttcctca ctacgtttca tcatttcttc atagaatgtt
     1981 tcaatgcaag aaagcaagtc ttcttttcta tagtagacat ttgccatcac agaacctaag
     2041 tccgcaagat cagcattttt cgggtcaaga atatacagtt ttgaatctgt atgaagcaag
     2101 gcttcaatca gtgtcagtat aaagtaagtt ttaccgccac ctgtaccacc agcaatcaac
     2161 atatgaggga gcttatcata ttcccaccat acgtttttca ttaagcgaag tttaccatct
     2221 ttagcttcta cttcatcaat agaaatacga ctggctatgg tgtcatagag caaagtatat
     2281 tccacatagg aatcctttaa ctctttatcc gtcagctcac agtacaagcc actctctaat
     2341 ttcttttcca agtgtaagag ttggtcttga tattttccca gcgtgatttc cacccgtatc
     2401 tgtatcaagc catttttaag tcgataatac attttaggga agtaggttat cttttccttt
     2461 gtacgaccag cactatcttt aaagaaaccc tctgttttga cctgttcaga ttcataccac
     2521 ttgttttcaa gtatcatctt tgccagtttt tgacggtggt aaagttgttt aaccgtatca
     2581 tagcgaaccc gtttgaatac aaacgctacc agcaagcaga taagaattgc gacactgaaa
     2641 ctgataatta aatagggaat gtcaatctta tctgcttgtg ataggttaaa atcctgccag
     2701 ttgatctgct ggattgtctt cacatgaaac agtccgacaa ccagcaggaa aacaggcagg
     2761 agtgacgcta tcgtaaaatg aaagactaaa tctttaccag atgggcgaat ccttttacca
     2821 cgctgtttca tgcgaaaaag tctcctttct acctagcgac tatttgtctt gtgtcggttc
     2881 tttctttgct tgtggttgag ctttgaatga actagaatcc tttgtcagca caatatcgtc
     2941 tgccttgata taccagtcaa catctgctcc ttgataggtg gcagtagcaa cggtgtccgc
     3001 aatgggattg ataagttcca cccgtgcgtt ataatcaaac tctttcaaag gcacgctggc
     3061 aggaatactt acttgaatca tgcgtccttg tcctttggat tttaagtcat aggtacgttc
     3121 cttgatttca tctgaaaccg acccgtcttc attttggatt ctcacttcac gacgtagagc
     3181 agagaatttc aattctccaa aagtcgtgtc tttatctaat acaatgccat ttgctaatct
     3241 catcattttt cctctctttc tttattcttt tatcatgtcg tcagcatgta aaaggtaatt
     3301 tgtaaaacca cgagtgccga ttttgtagcc ctctgcggta atacgtggat tgactaactt
     3361 cacacgttcc tcaaagccga aatgtttttc gccagcttca gcaggaagca ccaccacaat
     3421 atcatctgct ctttgaacat cagaatagag attatagctt cttgataaga cagttagccg
     3481 tccgttgatt cttcgctgaa cgactttatc ctcgccagca aattctaaat tgccgaatgt
     3541 tttttccatg ttgggaatca caaatttaag ttccatattt ttacctatcc tttctttttt
     3601 attggctgaa tgaatgtttg atggtcttaa agagtgggga acgacctttt gattcttgat
     3661 tttttgtttt cataagttca cttcctttca aaatcgggta aaaaaataga cacctcattt
     3721 tttgaagtgt ctacctatta aatattcaaa ttttattgga agtatcttta tatcttcact
     3781 tttcaaggat aaatcgtcgt atcaaagctc attcataagt agtaaattag tagtaaattg
     3841 agtggttttg accttgataa agtgtgataa gtccagtttt tatgcggata actagatttt
     3901 tatgctattt ttatataaaa aaattttttt tactatgatg attaaaattt attaaaatta
     3961 aaatttattg acaaatataa aatttaaata tataattaat tcataaaata aaaatatgaa
     4021 aggatgatgt tgttcggtga tatttgatat cataaaatcg ttgatataag tattgctgga
     4081 aaagagtgaa taaaatcaaa tttaataagc aaacaaagta aagctatagt tttattgttg
     4141 tgcttaaaaa tatttggttc tattttactc ttatttaaac tcatcttttt gaagttaaac
     4201 cacagagtaa actctgtggt ttttttattt taaaaataaa ttttaaggag tggtatgttt
     4261 tgttaaacaa attatgtatg tatttagaaa gaccagagct ttataaacaa agtgaaatta
     4321 atttttggga tgatgagtat atttcaaagc aattattaaa agcacactta gacactaatt
     4381 ttgaaggagc aagtagaaat tttaacttta tcgaggattc tgtaaattgg atagttacag
     4441 ttgcaaatcc agctaattat cctaaattac ttgatttagg ttgtggtcca ggtttatatg
     4501 cagagaaatt tgcacaaaaa ggttataagg taactggaat tgatttctca aaacgttcta
     4561 ttaactatgc acaaaataga aataaggaga ctaatttaaa cataaattac ttgttccaaa
     4621 gttatttaaa tatgaactat aatgaggaat ttgatttggc tactttaatt tattgtgatt
     4681 atggagcttt gtcaactgaa aatagaaggt tattaatgga aaaaatttat gatagtttaa
     4741 aacctggagg aaaattaata ttagatgtat ttacaataaa taaatacaat aattttgagg
     4801 aaattaaata ttgggaaata aatgaagatg gaggattttg gagtaatgaa aaatatatgt
     4861 gtctacaaga taattgtaaa tacaatgatt ataatacatt agaacaaact cttgtaataa
     4921 ccgaaaaaga tgaaaatgta ttttatgttt ggaatcacta tttttcaaaa gaaagtttct
     4981 tattagaagt tgaaaatatt ggatttaagt ctgttgagtt ttttagtaat gttaagggtg
     5041 aagcatattc tgacgatagt atgactatgg gattagtttt acaaaaataa taattttttt
     5101 aaaatgaata aataaaaact tgaaaccttt aacaataaaa taacataata aaagcttaca
     5161 tattgaaaaa tggaaacttt tatgttattt ttagtacatt acaaatttgc ttatataaaa
     5221 atcaatactt gaattattat ttttatgtga aatttatcta tagtatattt ttataaacaa
     5281 gtaatgttat taaaattaag tatgtagtta aaagtgtata gcaaaaaggc tgttatatta
     5341 atgttagagg ttcagggaaa atatataaag caaagtattt acagataatg tagattgcac
     5401 agctataagt tagataatag aattatgtaa tcaagaattt acaacaaagt ataagataag
     5461 gataatgcta aattgccata caggtgctag atgtgtaaat ggatttaatt aatactattg
     5521 ataaagtgca acaagcacat aaaccaattg atgagattat taaaactata agagatatag
     5581 ttgacataat taaaatattg gaacttgttt ataattttaa atcaaatcaa taatgattaa
     5641 gattaaaata atattaaggc atcaatgtta taagagtaca atataaggag tactatatag
     5701 tactccttac ttatacatat taggcaatag gaaatttttt catagttaaa ttttcaagta
     5761 ttttttagga aactttataa ttgagaatta agggtatagt atgtgatgaa cttactgaat
     5821 ttataattag gttatataat ggaataatca tcaatagttt tactttaggg aaaatatatg
     5881 cttaaggaag tatagcttac taatattatt aaaatgtata tttaaaatga taaaaaaaga
     5941 attagacaag tatttagtaa attgactatg caaaattcta tcaagcagga accctatgac
     6001 tttagtcatg ggaggttcag taggtaactt gttgaattaa gtttatatta tttaaataaa
     6061 ctacttaatt tagataatat aaaaagtcag aattttaaga atagagctat ataatcttag
     6121 ctattaggag aattactatg ataggaatat attttagtgg aacaggaaat tcaagatact
     6181 gtgtagaaaa atttttacaa gaatatgata taacagcaaa ttcttattct attgaagata
     6241 atgaactttt acagcacatc aataatcatg agaatattat ttttagttat ccggtgcaat
     6301 atagtaatgt gccaaaaata ttgaaagatt ttataataaa tcattcaact ttgtggaatg
     6361 gtaaacgtgt ttttattatt gcaactatgg ctctatttag tggagatggc tctggtgtgt
     6421 tatctagaat attaaaaaaa tacggtgcta taactgttgg tggattacat gtgaagatgc
     6481 cagatagtat aggagatgaa aaggtattaa aacattcttt agagtataat agaaagcttg
     6541 taataaaagc agaaaagaaa gtaactaaag ctgctaaaaa actgaaacat gggtcaccac
     6601 caaaagaagg gttaggtttt ttgagtcata ttgtagggtt atttggacaa cgattgtatt
     6661 ttatcaacaa aacaaatatg tatacagata aactaaaaat taatactgag gaatgtattg
     6721 tatgtggtaa atgtgttaat ttatgtccaa tgaagaatct agtaattaaa aattgtatga
     6781 ttgttgcaaa tgggcaatgt acaatgtgtt atcgttgtgt aaataattgt gctaaaaaag
     6841 caattacatt gttaggtaaa aaagttgttg aacagagtaa tattaaaaaa tatatgtaag
     6901 aaatttaaag ttttattgaa tgtaaattga tgtatatttt atgttagaaa gagaggttaa
     6961 ataatggaat actacatagt tgattcattt gcaactaaac tatttaaagg aaatcctgct
     7021 ggagtttgtg ttcttgacag aagaatacct ttagagttaa tgcagaaaat agcagaagaa
     7081 aacaatttac cagaaacagc atttgtagtt aaaggtaaag gaaattatga attacgatgg
     7141 tttactccta aagcagaaat tgatttatgt ggtcatgcaa ctttagcagc agcttatgta
     7201 atatcaaatt ttattgatgt taatgtaaaa aggatagatt tctttactca aagtggaaag
     7261 cttgaagtta cacgaaatgg aaatctatat gaaatgatat ttccagaaat aatgcctatt
     7321 gaaatagagt tatcaccaca acaagctaat ttgataggct gtgtaccaag tgctgtttat
     7381 tcttcaagag atttaatctt attgctaaac agtgaacaag aagttataaa ttataaacct
     7441 aattacgcac aattgcgtaa attgacagac tggttaggta taataatcac agcacaagga
     7501 agtaatacag attttgtatc aagatatttt tgtccagaat tagattcaga agaccctgtt
     7561 actggttcat cacattgtaa tcttattcca tactggtctg aaaagttggg aaaacacaaa
     7621 atggtagctg ctcaactttc taatcgtggt ggtataattc agtgtgaagt gttgaaggat
     7681 aatactgtga aaatttctgg tgaagcagtc ttgtttatgc agggaacaat aaagattgat
     7741 atttaaataa gtataataac taatttatta tgaaaaataa ttaattttct tagtgttaac
     7801 aataataaag tagagttaaa aaacttgatt tcacttaaat caatatgata gatgttgata
     7861 ctaagattga caagcatgat taaaaaagat aatatttaat ctataaagat aaaaacacag
     7921 tctggttggt agtccagacg cagcaatagc tgtcagtaac ctgcctcctt ggttgtccgt
     7981 tctttagaaa gtaatttaag gaggaactag caatggacaa aattagtgga aaattaacag
     8041 tcttatttga agaaccattt tgggtaggta tatttgaaag acaagatggt aaaaaatatg
     8101 aagcatgtag ggtagttttt ggagcagaac ctaaagaagt agaagtgtat gaatttatac
     8161 ttgaaagatt tttctcatta gattttggta gcattaaatt agaaaaaaat gtgaccaaag
     8221 acaacattgg atacaaaaga atgcagagaa aagtaaaaaa ggaacaagaa aaagaaacta
     8281 tagggactaa ggctcaaaat gctcttaagc ttcaatatga agaaagaaaa caggatagaa
     8341 aaaacctagc taaaaataga aaagaagaag aaaaagaaag actatttaat ttgaaacaag
     8401 aaaagagaaa agctaaacat aagggtcatt aattttaatt aaatatcttt ttaataattg
     8461 ctagaaaaag gtgtataaaa tttttaattt tatacacctt aatttattga aaagaataag
     8521 gttttatatt tcaattactt taaaatataa aaatatacta ataaaaattg taaaaatagt
     8581 ataaatggta taccaaatgt aaatttaatg tgtttagttt tatgtctaaa agaatacatt
     8641 ccaattatag aacctatact acctccaatt actgctatag acataagagt agcttctttt
     8701 attctccatt cgtttctaat tgctcttttc ttatctatgt acattgaaaa gaaagcaata
     8761 gaattaatca ctataaaata tattagaata aaatttttca cagtcttgcc tccataaaaa
     8821 tatattatac attataaaac aagatatttg ttttggaaag ttaaatttgt aattagtaca
     8881 tataaaatat ttgataagaa caaatataat attaattgga aaatttaaaa aaattaaaca
     8941 attagcaaat agggggtata tttataagac acaaagtgat aaaatctatt tagaaaataa
     9001 tgtaaaataa caggaggata aagcaaaatg aaatttatag tagaaaaaga agtttttgat
     9061 aaattagaaa atgtatgctt tggtgtagta gttgcaaaag gaatagataa tactaaagaa
     9121 atagaaagaa taagcaacct tttagatata agtatagata gagtagaaga ttacttcaaa
     9181 gataaaaaag taaaggaatc agaagaaatt ataccatata gagaggcatt tagaagtcta
     9241 ggcatgaacc caaataaatt tatgagttcg atagaagcaa tgacaacaag ggttgctaag
     9301 aataagaaac ttcctcatat aaatcctatt gtagatttag gaaactctat ttctttaaaa
     9361 tatttacttc caatgggtgc tcatgatatg gattttagaa atgatgatgt atatgttaga
     9421 ttttctaaaa aaggggataa atttgtacca tttggagaaa ctgatgttga attaatggaa
     9481 gaaggagaat taatttattc agttggagac atggtaaaaa ctagaagatg gatatggaga
     9541 caaggagaag aaggaaaaat tactaatagc tcaaaaaata tattttttcc tatagatggt
     9601 tttactgatg ctaatttaga taaggtaatg agtgcaagag aagaattagc aaaattgtta
     9661 aaggaaatat ttaattgtga aattaaagta ggttttgtag ataaagataa tcctgaaatg
     9721 gaaatataat aagttgttga atacttgtta agatattata tttacttaat gatgttaaaa
     9781 aggtattaaa aaagaaaggt cgtagtaaat ttgaaaaaaa ctataataaa gaatgtaaat
     9841 ccaatgattg aaaataagtt tataggactt tttgaaatag agtataaaaa caaattagat
     9901 gaagataagg tttggatggt agcatcaaga aaaagtagtg agcaattaaa aaatatttat
     9961 ttgaagaatg aagaagatag tgtagatgca gttgtaatag ttgggcttca taaaagtagt
    10021 aaaaaattaa ttttaataag acaatttaga gtacctatta atggatatat atacgagtta
    10081 ccagcaggtc ttgttgatga aggagagtct atagatattt ccgttgaaag agaacttaga
    10141 gaagaaacag gacttactct tttagaaatt aaaaaaaata aaagtattga taaagtttat
    10201 ttatctcctg gaatgagtga tgaatctgtt gcctttgttt attgtatatg tgatggtgat
    10261 ataacagatg aatttttaga accagatgaa gagattgaag cgcttttaat ttcgcaagaa
    10321 gatgcaaaag agattttaca aagtaatcat aagatagaca caaaagcatt tttgatttta
    10381 caaatgtttg tgagtttagg agctaaatta tttgaataga tatatagaaa agaggttata
    10441 tagaagagta aaataataaa gtttatttta cttgtgtaaa ttataatctc tttttttata
    10501 caataaagta taattttgtg tatttttaaa taagtgattt gactcttaaa ttttcataat
    10561 atgttataaa ccaatttaag atactacaat aattaaaatt ccaaaattat ttaacatatt
    10621 tgcaattaaa atgagaaaat gtaaaggctg aatgattgaa aaatttgatg taaaatagta
    10681 gcttagttta atactatagt ttgtagggtt aaaaattaga aattgtaagg gagataaatt
    10741 ctatgaaaaa attagttatt attttaatta gtatcattag tatggttagc ttgacagctt
    10801 gtaaaaaaag caatgaaagt aaatataata atttaaacaa caaagaatcc ggacaaacaa
    10861 ccattgaaat ggacttagat aaaaattatg atacttcaga cccatttgtt aatgcacgtt
    10921 tgttttgtgt gtctaatgat atagatgttt tagacacaga gatttctttt aaaatggatg
    10981 gagatagtgg tattgtggaa ataaaggaca ataaaacaga tgaaacttta tggagtaata
    11041 catggcatgg aagcgttgat aatgatacat tcacaatttc tctttctaat atacaaaaag
    11101 agaaagagta tgctatatgc tttactggaa ttaaaataaa tcatgcagtt gtaaaggtta
    11161 cttttgaaag caatttagtt aaggagaaag aaaaaccctc aaaataaatt ccagcttttc
    11221 tataacttga tattcagtac ataaaagcag ttgatattta tgattgactg ctttttctta
    11281 tcaaaaagga taggttgtgc atttttactt gaaatagttt atgcacatca aaagttggtg
    11341 agcatgatat tggaaatggt aagcatgaaa tcttgataat acaaggaaat aaatttaagt
    11401 tttcaattaa gtatatttcc ataatttagt taacaataag ctaattatta tttatatttt
    11461 gatatataaa taataattgt agcaaatttt aaaaaatcat atattttttg aactaaataa
    11521 aaatttattt ttattatttt aaattagagg agattcttga agatatatta tttttaaaaa
    11581 taagtatagt ttaaaaattc aaaaaaaaga tggcaaaata tgtcccaagg gacattgagt
    11641 aaaataaatg atttcagctt atttacacta aaaagtacat actaaatgag gggtaaaata
    11701 aagaaaaatg atatactaat tgtcttttga taataaaaaa acatggaatt tgattattta
    11761 atcaagaaaa agtattgaac aaatgattta tttagtgtac aataatcatg taaaaacata
    11821 gtaatggagg gagacatttc ataatgaaaa ttaaggtagg aatgaacgga tttggaagaa
    11881 taggaagagc tgtattaaga atagctcaag aagaattagg agataatata gaaattgtag
    11941 ccataaatgc aagagcaaca acagaatctt tggcacattt atttacatat gattcatgct
    12001 atggaacttt tagaggtgaa gtagaggcaa aggatgaaga cactctagta gtaaactcta
    12061 aagagataaa aatattaaga tataatgacc cagaagaatt accttggaaa gaattggggg
    12121 ttgatatagt aatagaatca acaggattat ttacacaaag agaaaaggca gaaaaacata
    12181 ttaaagcagg agcaaaaaaa gtaattataa cagctccagg aaaaaatgaa gatataacta
    12241 tagttatcgg agttaatgag aaagaatatg ataatgaaaa gcacaatata atttcaaatg
    12301 catcttgtac tacaaattgt ctagctcctt ttgctaaagt attggacgaa aaatttggta
    12361 tagtaaaagg gttaatgact acagtacatt catatactaa cgatcaaaga atattagata
    12421 aaagccataa agatttgaga agagcaagag cagcggctga atctataata cctaccacta
    12481 caggagcagc aaaagctgtg tcaagagtac taccacaatt agaaggaaaa ttaaatggtt
    12541 tttcacttag agtaccaaca ccaacagttt cacttgttga tttagtgtgc gaacttaaag
    12601 aaaacgttac atctgagcaa gttaactctg tgttaaaaga tgctgctgaa ggagaattaa
    12661 aaggtgtttt aggatattgt gacaagccac ttgtttctat agattataaa ggagattcaa
    12721 gatcatctat tatagacgct ctatccacaa tggttataga agacaatatg gttaaggtag
    12781 tttcttggta tgataatgaa tggggatatt cttcaagaac tgttgactta gtaaaatatg
    12841 tagctgaaaa attaaaataa tatatatttc ttaaagcaaa ttttaaaaaa attttttaaa
    12901 aaaattatat aaaactaaaa aaaaagatta tgaaaaatat tgaaggaaaa attttttcat
    12961 aatctttttt ctttcatgca aaaagataca agttacaact tatatttgca tattaggtcg
    13021 ttagagaagt atatatatat ttagtaagga atcatacttt ttataaaatg aaagataaga
    13081 tgtcgaaaac ttgcataaat ctgaaaaatt tttattatta aaatactgat ttccatttta
    13141 aaaatgataa aaaaatgaca aataaagttg caaatagtaa tacttataag tataattaaa
    13201 aaagagaaaa ggttttgtta aactttataa caaaaaaaga agggatgatt caaaatggag
    13261 aagttaacag acaggagaca ggttaaggca gtaagcatgg aaaccttcat tttcctaata
    13321 ttactagtag ttggttttgg gtatgttggt agtattatgg gagcaggaat gatgttcaaa
    13381 gtaataatga gtacagcaca tgctttacta ttggagacag ttttcttaat tatggctatg
    13441 gcagttcttg caggcgcatt aagcgcacta ctttcagagt ttggagtaat agcgttaatt
    13501 aataaaattt tcgctgtatt tatgaagcct ttatatggac tacctggtgc tagtatagca
    13561 ggagctataa caacttattt atcagataat ccagctatta taccttttgc taaagataaa
    13621 acattcacac agtattttaa acaataccaa gttccagcat tatgtaactt aggaacagcc
    13681 tttggtatgg gacttatctt aacaacattt atgatttctc aaggtacaga atatgtactt
    13741 ccagcattaa ttggtaattt aggtgcaatt ataggtagtg tcataagtgt tagaataatg
    13801 ttgacattta ctaagaagtt ttataaatac aatccggaag aagataaagc aacaggaaca
    13861 ttagagaaaa aggatgaatt tagagaaatc agagaaggaa atgtatttca aagagcttta
    13921 gatgcaattc ttgaaggtgg taaaatgggg gtagatatgg gaatggctat aattcctgga
    13981 gtcctagtag tatgtacatt ggtgatgtta ttaacttttg gaccatctac agatcctgtt
    14041 acaggtcaag aagtttatac aggagctgca tatgaaggaa taaaattact tccagttatt
    14101 ggagataaac ttggatttat attagaacca ttatttggat ttacatcacc agaggcaata
    14161 gcattcccta taacttcatt gggagctgtt ggcgcagcaa tgtctctagt tccagagttt
    14221 ataaagtcag gagctataac tcctaatgat atagcagtat ttacagctat gggtatgtgt
    14281 tggagtggat atttaagtac acatattggt atgatggatg cattaaatgc tagacagttg
    14341 gcaggtaaag ctattctttc tcatacaata ggtggtttat gtgcaggagc agctgctcac
    14401 tttatattta cactcgtagg ataaatgtaa tcagctaaga taaaaaataa tatacaaaaa
    14461 atggttgttg atatgtaata agaatttaca tatcaacaac catttttttc tgactaaaga
    14521 taatttataa tacataaaca ttaagtggat attagagtta agttatattt atgtaaaaag
    14581 taacaaaaat ctgtagaaat ttatgacaaa atttagtaca ctaactatat gttcttattt
    14641 aaaatttaag gttagaatta ggaattacct agtagagtaa gctttgatta ataaaagaat
    14701 cataaaataa aaatattagt cataaagatt ataaataact tacttaaaat aaagccatag
    14761 aacttacaaa aattaaaaaa gcatataaat atggaggggt acaatggatg aattatttca
    14821 tataggaatt gatgtaggtt ctacaacggt gaaggttgtg gtacttaata atagtaataa
    14881 tatagttcat aaagaatata gaagacatta ttctgatgta aaaaaatcag ttaaagaagt
    14941 actaaatggt atatatgaaa aattgggaga tataaatact accattataa taactggttc
    15001 aggtggaatt ggaatatcaa aaaaattagg tgtaaaattt gttcaggaag ttatatctag
    15061 tacaaaatca atagagtatt ttcatcctga aactgatgtt gtcatagaac ttgggggtga
    15121 agatgcaaag ataacatatc taagtggagg aatagaccaa agaatgaatg gaatatgtgc
    15181 aggtggcact ggagcattca tagaccagat ggcatcactt ttaaagacag atgccagtgg
    15241 attaaatgaa cttgctaaag ggtataatgt tatatatccc atagcttcaa gatgtggtgt
    15301 ttttgctaaa acagatattc aacctcttat aaatgatgga gccaatcaga ctgatatagc
    15361 aatgagtatt ttcaatgcag ttgtcgtaca aactgtgagt gtcctttctt gtggaagaaa
    15421 aatagaagga aatgttgcgt ttttgggtgg tccattgtac tttttatctg aacttagaga
    15481 agcatttaaa agagtattag atttaaaaga tgaaaacata atcttcccag aaaatgcaca
    15541 actttatatt gctattggag caggattatt atcagtagat gaagataata tacaactaaa
    15601 atctctaata gaaaaattgg atagtataaa gaatattgaa gatggagaag taaatttatt
    15661 agaaccttta tttaaagata aaaatgagta tgaagaattt tcaagaagac atgaaaaaga
    15721 aaaaataaat tatgtggaca taaattcaat aaaaagcaat tgttatttag gaattgatgc
    15781 aggctcaact acaactaaag cagcacttat tgatgaggat ggaagacttg tatattctta
    15841 ttataatagc aatgaaggaa atccattaaa aacaaccatt aaagttataa atgaagtata
    15901 tgatatatta ccaaaaaata ttaaaatatt gagttcaact gtaactggat atggagaagg
    15961 tcttattaaa aaagctctta aaatcgataa tggagagata gagactatag cacattacaa
    16021 agctgctaaa tttttcaaca aagatgtaga ttttatactt gatattggtg gtcaagatat
    16081 gaagtgccta aaaataaaag atggagtaat tgataatata atattaaatg aggcttgttc
    16141 atcaggttgt ggctcatttt tagagacatt tgcaagttct ttatctatga caatagaaga
    16201 atttgcgcat gaaggtattt attctcaaaa tcctgtagat ttaggttcaa gatgtactgt
    16261 atttatgaat tctagagtaa aacaagctca aaaagaaggt gcaagtgttg gagatatatc
    16321 agcagggcta tcttattcag taataaaaaa tgctttattt aaagtaataa agattagaga
    16381 tttaaatgag attggtaata atattgttgt acaaggtgga accttttata atgatttggt
    16441 gcttagaagc tttgaaaaac taattggaaa aaatgtaatt agacctaata tttcaggaat
    16501 tatgggagct tttggttcag cacttatagc taaagaaaaa tatgaaagtg gatacaaaac
    16561 atccttgtta tctaaggaag agttgaataa tatcaagcta gaagcaagtg taactagatg
    16621 taaaggttgt tcaaatcatt gtcttcttac aataaataaa ttttctgata atgaaatttt
    16681 tatttcaggt aatagatgtg agaaaggtga agctatttat ggtgataaaa aaataggatt
    16741 agaaaagaaa aaaccaataa atttatttaa gtacaaatac aatagaattt ttagatacaa
    16801 accattggaa aaagaagaag caaaaaatgg ggaaataggg ataccaagag tattaaatat
    16861 gtatgaagat tatccatttt ggtttacttt tttcaatgct atgggcttta gagttgtact
    16921 ttcagatagg tcatcaaaac agttatatga aactggtata actagtattg catctgaaac
    16981 agtttgttat ccaggaaaat tagttcatgg acatatagaa aatttaattg caaaaggtat
    17041 aaaaaatata ttttatccaa gtgtaacaaa tgaaaataaa gaggattcaa atgcagataa
    17101 ttattataat tgtcctgttg taatatcata ttctgaagta ataaaaaaca atgtagagaa
    17161 tataagaaat aagaacataa attatataaa tccatttata agtctaaatg acaaggaaaa
    17221 attgaagaaa agactctatg atgaattatc aagacatttt tctgatataa aaataactaa
    17281 agaagaagtt aatcatgcag ttgataaagc tactgatgaa cagaatttat ttaaaatgga
    17341 gatacaagca gcaggtgaaa gagcacttaa agaaataaaa gaaagaaata tgaaggggat
    17401 tgtgttatgt ggaagaccat atcatataga cccagaaata aatcatggaa tgccagagct
    17461 tataaattca ttagatatgg cagtgcttac tgaagactct atatgtcatt tagccaatgt
    17521 acaaagacca cttagagttg tagaccaatg ggtatatcat tcaagattgt ataaagcagc
    17581 tagttttgta agagataaaa actatttgga acttgttcaa cttaattcat ttggctgtgg
    17641 attagatgca gtaactacag accaggttca agaaatactt aatgaaaaat cgaagatata
    17701 tactattata aagattgacg aaggcaacaa tttaggagca gctaagataa ggatgaggtc
    17761 cttaaaagca gctatgtttg agagagaaag caaaaatata gatgtaaaaa atataaaggt
    17821 aaacaggata cagtatgcta aaaataatca gataacgaac aaacatacta tattagcacc
    17881 acaaatgtct ccaatacatt tccaattcct tgaaaaagct gttaacttaa gtggttataa
    17941 tatagaaatt ctaaaagata ctgatagtag tgtaattgaa gaagggttaa gatatgtaaa
    18001 taatgatgca tgttatccag ctataatagt tgtaggtcag ctaataacag cattaaaatc
    18061 tggcaaatat gatttaaata acacttctgt tactataact cagactggtg gaggttgtag
    18121 agctacaaat tatattggat tcttaagaaa ggctatatac gatgctggat ttaaagatat
    18181 tcctgtcatt gcacttagtg taaatggaat agaagatagt ggtataatgg ataatatatc
    18241 actaaaatta atcaatagac tctttatgtc tgttgtttat ggtgatttgc tgatgaaagt
    18301 actatataga gtaagaccat atgaaaaagt tactggaagt gcaaatgctc tatatgaaaa
    18361 atgggttgat atatgcaaat cttctcttgt taaagcaaaa atttctgtat ttactagaaa
    18421 tataaaagat atagtaaaag aatttgataa tttagagata ctagatatca aaaaaccaaa
    18481 agtaggtttg gttggagaaa tattggtgaa attccatcca attgcaaata ataatttagt
    18541 agatatatta gagcgagaag gtgctgaagc agtcgtgcca gaccttacaa atttcttctt
    18601 aagttgtgcc tttaatacta tatacaaaca cactcattta gaaggaagta gaaagagtag
    18661 aatgatagga gaagcattta tatacataac tggaatatat caaagagtat ataaaaaagc
    18721 acttgataag agtgagagat tttatgctcc agccaatata aaaaatgttg ctaaaagtac
    18781 agaaccagta gtgtcattag gtaatcaaac tggagaaggt tggcttttaa caggtgagat
    18841 ggttgaatta ttaaatgaag gtgtagaaaa cattatttgt atgcaacctt ttggatgttt
    18901 acctaatcac ataattggta agggttctat aaaagaatta aagaggttgt ataaaaatgc
    18961 aaatataata cctattgatt atgacccttc tgcaagtgag gtaaatcaac ttaacaggat
    19021 aaaacttatg ctttctaagg cttttaaaaa tatataagat ttatattttg atatgttaaa
    19081 agttaatgaa tttaaaaata tactctatat taagtatcta taaaaaagag ttatcctatt
    19141 ttttagggta actctttatt agttaaaaaa ttatatgtat actgatatta aaggggggaa
    19201 gtaatatgag tttagtatat gatgtttata aatcaaaatt aggaaatttg tacatattaa
    19261 gtgaagatgg ttttatagtt agtatataca taggagatga aaaatttaat aatttaaaaa
    19321 atgaatgtat taaaaaagga attgatataa agagaagtaa ggaagattta aaggaagcaa
    19381 taagccagat agatgattac tttaatggaa aaagaaaagt ctttaattta aatattcgta
    19441 ttgaagggac tgaatttcaa aaatcagttt atagggaaat gttaaaaata ccttatggag
    19501 aaacattgtg ttattcagat atagcaagaa atataaacaa tccaaaatca gtaagagcaa
    19561 taggacaagc aagtaaaagt aataaaattc caataataat accttgtcat agagtagttg
    19621 gcaaaaaaga tattggtgga tatatgggga atcattctga tttgaaagag atactattaa
    19681 atttagaaag acagagttaa cttagaaaga aatgttttta ttgttaatat ttgatataat
    19741 aatagtaaaa agaagtattt tttacaaaaa caatgaagaa tttattttaa tttataaaaa
    19801 tgagggggaa ttttaatgtg tgataaagtt atagcatcaa aatatcatga gaagggattt
    19861 aattgtgcag aatcagttat aaaagcatat aatgaggaat ttaatacaga tataccagtt
    19921 tgtttaggaa gtggactggg ttcagggtgt ggggttgcta gtttatgtgg tgcagtaaat
    19981 gcatcaaata taataattgg atatgtaaaa ggcagaaacc acgaagatga aagtactaaa
    20041 gctaaagtat atgctaaaga tttaactaca acagtaagaa aagagtatgg ttcagagtta
    20101 tgtatagacc taaaaaaaga tttagttgct tgtagagaaa taatggattt cgcatatgat
    20161 agtctaaagg aaactttaaa aaaagaatta taagaaaata agctatcaag tataaattat
    20221 ttgatagctt atttttatag tcgatactaa ataaatcaaa tatattttag aattattatt
    20281 tgtaaa
//
LOCUS       DAEOSP010000054        20166 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.054, whole genome shotgun sequence.
ACCESSION   DAEOSP010000054 DAEOSP010000000
VERSION     DAEOSP010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20166
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.054"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(84..311)
                     /gene="acpP"
                     /locus_tag="KRH54_001602"
     CDS             complement(84..311)
                     /gene="acpP"
                     /locus_tag="KRH54_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG8579269.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            483..908
                     /locus_tag="KRH54_001603"
     CDS             483..908
                     /locus_tag="KRH54_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579270.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            956..1162
                     /locus_tag="KRH54_001604"
     CDS             956..1162
                     /locus_tag="KRH54_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579271.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            1344..1523
                     /locus_tag="KRH54_001605"
     CDS             1344..1523
                     /locus_tag="KRH54_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579272.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            1557..1757
                     /locus_tag="KRH54_001606"
     CDS             1557..1757
                     /locus_tag="KRH54_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579273.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            2010..3026
                     /gene="ccpA"
                     /locus_tag="KRH54_001607"
     CDS             2010..3026
                     /gene="ccpA"
                     /locus_tag="KRH54_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBG8579274.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(3149..3547)
                     /locus_tag="KRH54_001608"
     CDS             complement(3149..3547)
                     /locus_tag="KRH54_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579275.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            3759..4664
                     /locus_tag="KRH54_001609"
     CDS             3759..4664
                     /locus_tag="KRH54_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579276.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            4779..4959
                     /gene="ssrS"
                     /locus_tag="KRH54_001610"
     ncRNA           4779..4959
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KRH54_001610"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5142..6359
                     /gene="cdeC"
                     /locus_tag="KRH54_001611"
     CDS             5142..6359
                     /gene="cdeC"
                     /locus_tag="KRH54_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBG8579277.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNSECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(6459..7781)
                     /locus_tag="KRH54_001612"
     CDS             complement(6459..7781)
                     /locus_tag="KRH54_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBG8579278.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(7832..8626)
                     /locus_tag="KRH54_001613"
     CDS             complement(7832..8626)
                     /locus_tag="KRH54_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG8579279.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEQMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTVLDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            8736..10181
                     /locus_tag="KRH54_001614"
     CDS             8736..10181
                     /locus_tag="KRH54_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG8579280.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            10210..10419
                     /locus_tag="KRH54_001615"
     CDS             10210..10419
                     /locus_tag="KRH54_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBG8579281.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            10439..11833
                     /locus_tag="KRH54_001616"
     CDS             10439..11833
                     /locus_tag="KRH54_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG8579282.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSRNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNIPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            12073..12825
                     /locus_tag="KRH54_001617"
     CDS             12073..12825
                     /locus_tag="KRH54_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8579283.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            12851..13264
                     /locus_tag="KRH54_001618"
     CDS             12851..13264
                     /locus_tag="KRH54_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8579284.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            13268..14011
                     /locus_tag="KRH54_001619"
     CDS             13268..14011
                     /locus_tag="KRH54_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBG8579285.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            14011..14481
                     /locus_tag="KRH54_001620"
     CDS             14011..14481
                     /locus_tag="KRH54_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8579286.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            14498..15256
                     /locus_tag="KRH54_001621"
     CDS             14498..15256
                     /locus_tag="KRH54_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8579287.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            15240..16082
                     /locus_tag="KRH54_001622"
     CDS             15240..16082
                     /locus_tag="KRH54_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG8579288.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(16242..17147)
                     /locus_tag="KRH54_001623"
     CDS             complement(16242..17147)
                     /locus_tag="KRH54_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG8579289.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            17509..18243
                     /locus_tag="KRH54_001624"
     CDS             17509..18243
                     /locus_tag="KRH54_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579290.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            18454..19635
                     /locus_tag="KRH54_001625"
     CDS             18454..19635
                     /locus_tag="KRH54_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBG8579291.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILDLEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            19619..>20166
                     /gene="mobB"
                     /locus_tag="KRH54_001626"
     CDS             19619..>20166
                     /gene="mobB"
                     /locus_tag="KRH54_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBG8579292.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNV"
BASE COUNT         7932 a         2185 c         3504 g         6545 t
ORIGIN      
        1 tgtaatctag tctgtgcgaa ctctaatata atttattatg tagaagttta cacagactat
       61 ttatatttgt agacaaaacg atactatttg ttttcttcga tatatttaca tatatcacca
      121 atacatttaa agttctcagc ttcctcgtct ggtatttcta ttccaaattc atcttcaagg
      181 gccattataa tttcaacagc atctaatgaa tcagcttcta agtcgtccat taaagatgct
      241 tccatagtta tctcatctaa attgtctacc cctaattgtt ctgctattat ttcttttatt
      301 ttctcaaaca ttttaagcct cctaaagcag ttttaattaa ttttaatgtt agtttaatat
      361 aatatcaaga atttttcaat atggaatcta taaaaaataa taaaatttta taaatgtagt
      421 aataaagtat tatttgatga ataaacttta ttgataacaa atataactta ggaggaaatg
      481 ctatgaaata tgtaggacta ttactaactt cagtaggaat gtttttactt atagcagtaa
      541 atttttatta taattcaata actttagata tgcaacgtat agaggattat gtaatggaga
      601 ccaatttaat ccttgaagat gtagctgaaa aggagagtta tgtatctaat gaaaaagaag
      661 actacatatc aaggttaatg catgtaaaga aaggaataga aaattcaaaa acatccttct
      721 tgatagaaag atataaggag tacaaaataa aatcaataga aagtttaatc tatactatat
      781 cagaggagaa aaaagattat ctggatgaag ttgatagata caataaactt ggtgaaaaag
      841 aaataaataa actaatcaat aaaaactttt tagaagtaac ttatctgtct attactacat
      901 atatttaatt aaagagacaa aacttagtct cttaatgatt caaggaggat tagttatgac
      961 aaaattagaa gacaatatgg aacgtttctt tggaggatgc gaaggatttt ttgggaataa
     1021 aatgttgata ataatagtta tagtattctt actattatgc acagacattt tagaagactt
     1081 attatgcgat gacaatatat ggatatgggt tatattaata gtattgttat tatttaactt
     1141 cgatgatggt tgttgttgct aaaaaatatt gattattaaa taggtaaaac cagtaactta
     1201 tacaagttat ggttttacct tttttagttc aaattaaaaa atttaaaaaa atagtaaaaa
     1261 tacattagca aaatggaaac caaatgcata gtatatgtta ggagcgaaaa gctcaacaaa
     1321 atatcaacag gaggtaacgt ataatgttag atagattctt tggagaagaa ggatgttttg
     1381 gtggaggagg atggtggata atagtactat tcttcctatt cttagctttt ggagatagct
     1441 gggcagaatt tgacataatg gcatggatac cattcctaat agttttatta atactttgct
     1501 gctgtggtgg atgcttagaa taagaacaca gttattaaaa aaaggagggc tttgttatgt
     1561 tagatagatt ttttggagaa gaaggatgct ttggaggagg aggatggtgg ataatagtac
     1621 tattcttcct attcttagct tttgaagatt gctggagaga cattgacata atgtcatgga
     1681 tacctttctt aatattattg ctaataactt gttcatgtgg tggattcttt gatggtgaag
     1741 taggttgcgg ctgctaatac ttaaatcatt agaataaaaa aatgctggta ttttatacca
     1801 gcattttttt attttatttt ttaatatgat ttaaattaag aatgaatatt attaaatttg
     1861 ttgaatatag ggtataatat aaaatataat gtatttaaat gtacttgctt ttatggtaaa
     1921 ataaaaactt atataaatgt agttaaaata ggaaccttca aaaatttggt tcagttttag
     1981 ataaatttag taaaataagg aggatgaaga tgaaaggcaa tataacgata aaagatgttg
     2041 ctaaacaagc aggagtgtca atatctactg tatctagagt tataaatgat tcaaaacctg
     2101 taactgatga agtcaaacaa aaagttttag aggttataaa agagactgga tatataccaa
     2161 atccacttgc tagaagctta gtaacaaaga agagtcaatt aataggggta atagttccag
     2221 aagtttcaga ttcttttgtt aatgaggtgt taaatgggat agaagaggtt gctaaaatgt
     2281 atgactatga tattctttta gcgaatacat actctgataa ggaacaagaa cttaagagta
     2341 taaatctatt gagagcaaaa caagtggaag gtatagttat gatttcatgg atagttgaac
     2401 aagaacatat caactatata caaaattgtg gaataccagc gacatatata agtaaaactg
     2461 ctagaaatta tgatatatat acagtaagta ctagcaacga agaagctact tttgatatga
     2521 cagagcatct tataaagaaa ggtcatgaaa agatagcttt tataatgacg agtaaagatg
     2581 atactgtttt agaaatggaa agacttgctg gttatgagaa agcactttca aataacaata
     2641 tagaattaga caagagtttg attaagtatg gtggaactga ttatgagagt ggatacaata
     2701 gtatgaaaga actattagat gatggaataa tacctcatgc ggcttttgta acaggtgatg
     2761 aggctgccat aggtgctata aatgctatat gtgatgctgg atataaggtt ccagaagaca
     2821 tatctgttgc aggatttaat gatgttaaga tagctagaat gtatagacct aaacttacta
     2881 cagtatatca acctctatac gatatgggag cagtagcaat aagaatggtt ataaaattaa
     2941 taaataagga attaattgaa aataagaaaa tagaattacc ttatagaatt gttgatagag
     3001 aaagtgttac agaaagaaaa aaataaaact attaaaatca atcaggtagt gcctaaggca
     3061 ctacctgatt ttttatgtca ataatatcaa atttaataat agattaacta aaaataatta
     3121 tctaattact taaatagttt atttttatct attcatgagg gttagacttt ttagttttct
     3181 taaataaact tttagatttt ttttgttgac tacctggtgt attttttggt gcaggtgaat
     3241 ttgacttacc tttagtaggt ttagccttct tagttttagc tggtttagta tctctttggt
     3301 tttctgcatc tgagattttt tccattatat tatccagttt tttgcgttga ttttcatcta
     3361 aaaatgcttt gaattttgat actacttcat ctctgccatc tagattctta cctattttta
     3421 taagttcctc cataagttca ttttctgatt ttcctttgta actatcagcc atcttttcta
     3481 ttttatcctt gtcaatattt ttttgtttag ccattttttc taatgactct gtatctaatt
     3541 ttttcataaa aaaacccctt tctataaata tatattgaat tatattcata ctatttatat
     3601 atagaaataa tattgaagtg gtgaattgtg agtgatatat aatataaata aaaattctta
     3661 tagataatat atatgcactg agctttaaaa atggaacatt aaaatatgaa aaacatactt
     3721 taatataggg caattatata atataagggg gaattgtctt gaataactta ctatttttga
     3781 ttggagtgat tgcattgagt aatggtaata tctctatatt tgatgataaa aataaatcaa
     3841 aaggaaataa tagagttaga aaaaacaaaa aaaatgcaaa tgataaactt tcacagtcta
     3901 cacatggcaa tttaaggttt gatgcaaatg atataaaaag aggtttaagg ctcatggatt
     3961 taagtgaaga agatttagaa atgggaatgg aaataataac aagaactaaa aaatacatgt
     4021 caagagatga aagaaaaata ctaataaaaa tagaaagtgt tttagattta gttagaggaa
     4081 taaaaaaatt aaataatata gatatggtag atacagaaga tgaaacggat tttttcagaa
     4141 aaatggatag tgatgataaa aagaatatga tgataaagga aattatagat gtattcccag
     4201 aaaaaagaaa aaattcagtt gaaaaagcta taggaatgaa aaagaagata gatttatttg
     4261 cagaattatt tttaccagat gactttggtg aaggtggatt tagtttgagc tctttggcaa
     4321 atataaacaa tcttggtagt atgaataatc tcaaattgtt aggaagtctt ttaagaggtg
     4381 atgatagtaa taatgatgat gaagacaata atgatgagtt aaattatgaa gacgaagaat
     4441 tagattgtga agacaaagaa ttggatgatg atgagttaac agatgaaggt gaaatggagg
     4501 ataatgaaga tgaattagta gaagataaga acacaggcta tgaaaatata gaaaatgatg
     4561 aagatatagt gtacgatgag gaactataca acattaatga gcaagaatcc gaatacaatt
     4621 ataatgaaaa aaatactaaa actagagcta agaaaaaacg ataattaact aaatacataa
     4681 agaaaaaagg aagaaaaagt aatgatagaa taagattatc tcaattacta tataaaacca
     4741 ctttaaaaaa ttcaaaattt attatataat aataatatga aatcctgaaa catacgataa
     4801 ggctttttta aattcaacct acaaatgaaa tacgggagac cgtgtctggt aaattataac
     4861 atgcctagtt agtttggacg ttaaaggtta ctggcaagtc acccacctgg gagtgcctag
     4921 ggtgtgaatt tacttagcca ccggtgtttc gggacaaaaa aagtgctact atttcaaata
     4981 gtagtacttt ttttattaat atgcattttt ctactaaata cgcctaatta ttgataaatt
     5041 caaaaagcaa gtaacaatta agattataaa agcatagtat taattaggct tcaaaaagcc
     5101 aaagacttaa taagaatcgc ttacagggag ggcaatgaaa aatgcaagat tataaaaaaa
     5161 ataaaagaag aatgatgaat cagccaatgt ctacaatgaa tgaagaagaa gtgtatacag
     5221 atgaaataaa ttcagaagac atgagaggtt ttaaaaaatc acaccatcat aatggatgta
     5281 atactgataa taagtgtgag tgccatgatg attgcaatcc atgcaaccca tgtaatccat
     5341 gtaaacctaa cccatgcaat ccatgcaaac ctaatccatg tgatgacaat tgtggatgcc
     5401 atgacaattg taaatgtgat tgtgaaccat gtgaaatgga ttcagatgaa tgttttgaaa
     5461 acaaatgtgg accagaatgc tgtaatccta tatctccaag aaacttctct gtatcaaatg
     5521 cagtgccatt tgcaatagag gctaatagaa tatttgatac tatgcaattc caaacattta
     5581 cagatgcaac aggaccaaat ggagagccat taacttttga aacagaagta gtagaagtat
     5641 ttggttcagt tccaagtgca ggtcaagcaa gtgtaactat agaaaaaata tgcttaagta
     5701 atgatggaat cgttatagac acaggaatga caactttaga agatttcgat ttagacccat
     5761 taggagatat agtaggaaga aactgtgaaa caacttttga atttgcagtt tgtggagaaa
     5821 gaaactctga gtgctgtaga caaggaaaag gcaaatcagt agcttataaa caaagaggat
     5881 taactgtagc agttcgtaat ttagtactag agctaagagg tagatgtgga tgtacagagt
     5941 tcgttgcatt agctttccca gcagttagag caggaggtgg atgtaagaga agagttgatt
     6001 atgtagaatt tacttttaac acactttcag caccaatatg cttgccagct gacggaagag
     6061 ctgttacttt aagacaagaa tatcaaacta acttaactgt agattgtata ggaaaatcta
     6121 tattaaaatt agaatgcaac gaatgttgtg aacctttcta tgaattaatt ataccaaatg
     6181 atatagattt agtactttgc ttacaagaaa cagttagcac attaataagt gaacaaatag
     6241 tagttttagc atcaccaaat ccaatccaac caagacttgt tgatactttc tctaaagtat
     6301 gtgatttttc gcaatgtgga cctaatcatg gaagtggaaa gccaagttgc cacagataga
     6361 taggtagata tatagacagt ttaaattata aataaaaatc atctaaggaa ggctaccaaa
     6421 gtgtataatt tacattggta gccttcacgt ttttataact agcgtataga ttttagtgat
     6481 ttaaaaagca ctatagtatg taatagacaa gtaaagattg atactatcgt cattgtgtat
     6541 atgtatgcat aaatattaag tcctggaatt ggaagaaata catataaagc tattagttga
     6601 attaacatgc cagtaatagt atttatactt gaagctaatt cttttcttat tgaatgtaaa
     6661 atgccagata gagtttgatt tagggacata aatagtggaa ctaaaaacat agcttttaga
     6721 tattctcctg caagtgtatt tttaaatact aatatacata aatcttttcc aaagaaataa
     6781 aaaaatattg atgatagtat gcctacaaat aaagtaagaa gtatggagta tttgattttt
     6841 ttaattacac tcttacgctt tcttagagcc atttcctgtg aaatagttgg tattaaattt
     6901 acaataagcg ctgaaccaac agtaaaaggt aaaaatataa aaggcattac catcccactg
     6961 acaataccat acatacttaa agattgttga taagtcattc ctgataatgc taatcttgat
     7021 ggtatcatca tagaacttat agattgcaaa agtatgtttg acattctatt acaagttatg
     7081 ggaatagaca tttttaatgt ttccatagaa gaattataaa aatcttttat gcttataata
     7141 tacctattgt caaatgatga atctcgccat aagcaaataa gcataaatat aatattactc
     7201 aactcaccaa tcgatatacc taatagagct atataacaat tcatagattt attatttatg
     7261 tacataacaa gtaaaaaaac aaataatatt ttaccaattt gttcaatgat ttgtccaata
     7321 gcagggattt ttacattctt tatgccatag taatatcctc ttagtacgtt tgaaagagtt
     7381 attataacta tagcaggaca aacagctagt ataaatagat ttaatttagc atcttttaaa
     7441 aatttaagtg ataaaaaaga actattgaaa gatgcaacta tagatataat tagtgctacg
     7501 aaaaatgata tatataaagt tgatataaat aaagcattag tattgtgtct atcgtttaaa
     7561 gcttttctct ttgcaactag acaactaagt gcagtaggta tacctgttgt tgtaacagct
     7621 aagcaaatca tcaaaaagtt aaaaatcata tgatagatac ctaaacctgt ttcacctagt
     7681 gctcttgata agaatatttt atataaaaaa ccaaatattc ttacaataaa attggatata
     7741 aataaaataa ctgttgatag tactaaataa ggtactttca aaaaaatcac ctccttatct
     7801 tttaaatata ttcacaagga ggtgtaaaaa tttataattt tataaattct ggtttcaatt
     7861 tattaaattt agatacatat ttaaatccca tattttttag taatgaatat atgtagtcaa
     7921 actcacatgc aacttgactt atatgatgtg aatctgaacc tgtggttatg atttcccctc
     7981 ccaagtcttt atacatttga agtatatact tagatggggt taggtctgga agattatatc
     8041 tataacaaga ggtatttatc tcgatacctt tgccatcata aatagcttgt ttaagagttt
     8101 cttcaattat atcagaaaat agtctatcat ctaaaacagt gtcataaggt gcataccttt
     8161 taattaaatc taaatgacct aagacagagt aatctttata attcttaaca atattatata
     8221 aataaagata gtaattttca taaacttcat gttgagtttt atctttaaaa taattaccaa
     8281 ggtataagtc cattgtatct atagcatgga tagaacatat tataaagtcc aatggatatt
     8341 gatgtgcttc ctttttgtat gtatccatta attgagtttg aactccaaat tcaattcctt
     8401 tttttattga tattttatct ttatatttgc tttgaagagt ctctaaactc ttaaaataat
     8461 cttcatagac aattgaaaaa gaatcatcag catatacatc atagtcaaca tggtctgtga
     8521 aacaaatttc ctccaaaccg agttcaatag atttttttac catttgttcc attgtagatt
     8581 ttccgtctgt agagaaactt gagtgcatgt gatagtcata aaacataaat atttcccctt
     8641 tttataaaaa ttttggtatt atatatacat actaaataat atcaaatata cataaaagca
     8701 tcaataaaat ataaatacaa tgagcggtga taaatttgga aaaatacaga atagatttaa
     8761 atgagaaaaa atcaactaaa tatatacaga tatttaatca tataaaagaa ttgataataa
     8821 ataaagagtt agtagaacat gaaaaattgc ctccaataag aaaactatca aattttttac
     8881 aagtaaataa cactactata gttaaggtat acgagctttt agaaaatgaa ggatatgtat
     8941 ataaaattat agggagtggt acttttgtat caagtttaaa ttcttataaa agcggtatag
     9001 ataacaggaa aaataagatt attgtgcaaa gagaagattt aattcatttt gacaatggga
     9061 atccatctaa tgatatgttt cctatcgatt catttaaaaa tgctgtaaat atggcattat
     9121 ctaaagatgg tagttcaata tttgaatatg atgaaggtct tggctctgaa gaattaagag
     9181 aaaaaatggt agagtatcta agtgatggaa atataaaaac tactaaggaa aatattcaaa
     9241 taatatcagg tgctcaacaa ggtatagata tagtttgtaa aggacttata agttattcag
     9301 atgttgtttt tatggaagaa ccttcttaca atggagcaat tgaagtgttt aaaagtagag
     9361 gtgcaaaaat aatatctata cctatgttag atgatggaat tgatataggt attttaaaat
     9421 taaagttgga aaaaataaaa ccaagactta tatacattat gcctaatttt caaaatccaa
     9481 ctggaatatc ttactcaaca tataaaaaaa agaaattgat agaattggca gaagaacatg
     9541 atttttatat aatagaagat gattttataa gtgattttgt ttttgaatca aaggacaata
     9601 gaacaattag aagttatgat gataaaaata gagtagtata tataaaaagc ttttcaaaaa
     9661 tacttatgcc aggtcttagg ataggaattg tagaaatgcc aaatgagttg ttaaaaaaag
     9721 ttttatgggc taaatattca tcagatatat caacaccagg tttaattcaa aaatcaatgt
     9781 attactatat gaataatttt gattggaaga attatttgag tcatatagag aagatatata
     9841 ctgataagta taaattggca aaaaacttga ttaatgacaa attgagtggt aaattaaaag
     9901 ttagaaaatc ttgtggagga ataaactttt ttttagaatt accaagagga tatacatctc
     9961 aagcatttac aaattttatg ttaaacaaag gagtgtcaat gcttccaggg acttattttt
    10021 ttgacaatct agtggatgat agattcttta ggataaatat agctcgtcca agcatggaag
    10081 aactagaaaa gggaatatct ataataagtg ataatataga tgagtttttt tgtgaatata
    10141 aaaacaagtt agatataaaa agcaataaat tattttatta aataaataga ggttttttag
    10201 gaggaaaaaa tgtttgacaa gaagaaatta gataggatta atgaattagc aaagaaaaat
    10261 aaagagggaa tcctttctgc tgatgaaatc aaagaaagag aaattttaag gaaagaatat
    10321 ttagaaaact ttagagccca ctttagatca agactagata gtgtcaaagt agtttcacca
    10381 gaagaatatg agcaatacat gaaaaacaat aaaaattaga acttagtagg aggaaaatat
    10441 gaacttaaag tacgaattta agaatgcttg ggattttgaa agaaaagaaa atactattga
    10501 acaaattatg caatattctc gcaattatat ggagttttta agtaagtcaa agactgaaag
    10561 actttcagta aaagaaatta taaaattggc taaagaaaat aactatattt caatagatga
    10621 agctatggaa aaaggaagca taagttgtgg tgataaaatt tatgttataa ataaagagaa
    10681 ggcagtagca ctttttgtaa taggtaaaaa ttatatagaa aaaggtatga agattatagg
    10741 aagccatata gattcgccaa gattagattt aaaaccaaat ccactgtatc aagagagtaa
    10801 tctaggtttt tttaagactc attattatgg tggaataaaa aaataccaat ggacggctat
    10861 tccacttgct ttacatggta tagttatatt aaatgatgga acaaaagtgg acatatctat
    10921 tggggaagaa gatagtgacc cagtattttg tgtaactgat ttattgattc atttggctgg
    10981 agaccaaatg caaaagaaac tatcagaagg aattagtgga gaagctttaa atatattaat
    11041 tgggaatatt cctttagaag atgaagaaaa agaacctata acagcaaata tattaaagat
    11101 tttaaatgag aaatacaata tagttgaaga agatttatta agtgctgaaa ttgaagtagt
    11161 tccagctggt aaggctagag atttagggct tgataggtct atggtgttgg gatatgggca
    11221 tgatgatagg gtatgttcat atgctgctgt aaaagctata ctagaaactg aacaaccaga
    11281 atttacttca gttgcattat gtgtagataa agaagaaatt ggttctaaag ggaatacagg
    11341 tatgcactca aagttttttg aaaatactgt ggctgaactg attgctttag aaggagatta
    11401 ttgtgatatt aaagtaagaa gagctttagc aaatagtaag gtattatcag ctgatgtttc
    11461 tgctggatat gacccaaatt ttggagaagc atatgaaaag agaaattcgg catatatggg
    11521 aaatggagta gttttaacta agtatacagg ttcaagagga aagagtggat gtaatgatgc
    11581 taatgctgaa tttatgtcag aagtaaagag aatttttaat aaaggaaatg tagtatggca
    11641 aactgctgaa cttggaaaag tagaccaagg aggaggcgga actatagccc atatattggc
    11701 aaatcaaggt gcagaggtta tagactgtgg tgttggagtt ttaaatatgc atgcaccaca
    11761 tgaaattgtc tctaaagtag acatatatga aatgtataag ggatataagg cattttttaa
    11821 tataaattta taagtaaaaa tataaatgta ataagtgaat aaaggctgtt gtataaacaa
    11881 tttaatctgt tgtacaacag tcttattttt atgtaaaaat agcaatttgt cacactagag
    11941 aaaataaaag aaaatactgt caaattaaaa attatttaaa aaaaaattga caacgatttc
    12001 atcttgttat aatatgtaat aaagaacttg ttatataatg ttataaaaat gttacatagg
    12061 gaggtgagca aaatggatga aaagagacaa aaaaaagaaa tggatttaga agtaaattcc
    12121 tttgttcctt tatatcaaca actatatgac aacataaaaa aacaaatagc atctggcata
    12181 tataaaccag gagataaact tccatctgaa ggagacttat gtaaagaatt taatataagt
    12241 cgtataactg tgagaaatgc acttaatgaa cttgtaaaag aagatatatt atgtaaaaaa
    12301 cgtggtaaag ggacttatgt aacaatacca gagagaatag aagcaacatg tgcaggaaat
    12361 agttttacta attcttgtca tcgtattaat gcaaaaccaa gtaccaaaat aatctctgtt
    12421 ttaataaaaa aagcagataa gcaagtagct gaagctttaa gtattgaact agaagagaag
    12481 gtaatatgta ttaaaagact tagattaata gatgaagtac ctgttatttt tgaagttgac
    12541 tattttagaa tagattacat gtttttatta aaagaagaat tagaagataa atctttaatg
    12601 gaggttatat ctaataatat aagcacatta cctaaacgag tagaaaatat atttgaagta
    12661 aaacattcaa acaaagaata ttctgaccat cttaaatgtg caagtaatat gccactattg
    12721 aaagtaagac aatcggtgta cacagaaaat aatgatgtct tatattacaa tgagcaattt
    12781 ataagaagtg ataagtacaa atatgcagta tcagcagaaa tataatctat aatttagtag
    12841 gaggatgagt atgaaaaaga taatattagc atctcatgga gacttttcaa aagggctttt
    12901 aaatgcagta aaaatgatag taggagattt agcagattgt gtcagttcgt atggtttgta
    12961 ccctggacaa agtgcttcag attttgcttt agaattagaa aaaacaatat cagaagataa
    13021 agaatgtgaa tatataattt tatctgactt atatggagca agtgtatgta cagcaatgtt
    13081 aagattaact aatctacata atgttaggtt attttctgga atgaatttga atatggtatt
    13141 agaacttctt acaaggtttt ctgatgaatt aacagttgaa gatatagacc aattagtgga
    13201 agaatctaga agaggaatac aaagcgtgac attacaaatt aaagaagaag aagaggtatt
    13261 ttagtttatg aaaaaaaagg gaatattatc agtatttaaa agtaaaaaac caattatagc
    13321 aatgatacac ttaaaaggag atactccaga agatatcttt gaaagagcta aaaaagagat
    13381 tacaatcttt gaagaaaatg gtgtagatgg aataatgtta gaaaattact atggtaatta
    13441 ttatgattta gaaagaatac ttgaatatgt atcaaaagct aatttatcaa taccatatgg
    13501 agtaaactgt ttaaatgttg atactatggg atttgaactg gcaacaaaat ataatgctag
    13561 ttatatacag gttgattctg tagtaggtca tgttaaacct agagatgaag ctactttaga
    13621 agagtttttt aaattacaac gttcaaaatg cccagcgtat ttaattggag gcgtacgttt
    13681 taaatatcag ccagtattat cagaaaatga tgttgaagaa gatttaaaaa taggaatgac
    13741 aagatgtgat gctatagcag ttacagaaaa tgctacaggt caagaaacat caatggaaaa
    13801 aatagaactg tttcgtaaaa atttaggaga cttcccattg gttatagctg ctggagttac
    13861 attggaaaat gcaaaaaaac agcttaaatt aggagatatg gcaataatag gaagttactt
    13921 taaagataat tacaaagatt ttggggatgt tagtgtagaa cacgtaaaaa cattcatgga
    13981 tgaaatcaaa aaaattaggg aggaattata atgattaaat tagtaagagt agaccacaga
    14041 cttatacatg gacaagtagc atttacatgg acaaagtttt taagtacaga ctgtatattg
    14101 atagcaagtg atgacttatt aaaagatgaa ctgagaatgg caggacttaa aatggctaaa
    14161 ccatctaatg ttaagttagt aatgaaaagc atagcagatt ctataaaagc acttaactca
    14221 ggtgttactg ataaatacaa cttattgata ctttgtgaat ctgtagaaga tgtttataga
    14281 cttgctaaag aggtaaaagc tattaaatct ataaaccttg gtggaacaaa atcagatgat
    14341 aatcgtgaaa atatatctaa agcagtgcat gtatcaaaag atgatataaa gatgattaaa
    14401 gagttagatt cagaaggcgt aaatgtattt gtacaattag ttcctgatga tgatgctaca
    14461 aatgtaatga aattaatata attttagggg gatgaaaatg gaatttacac aagttatttt
    14521 gataactcta attgcattct ttgcatatat gcatagtttt gtaggttcta caatgcataa
    14581 tagacctata gttgttgcac cacttgttgg tttagcacta ggtaatcttc atacaggtat
    14641 agtaataggt tcaacattag aattagtatt tatgggtgca tttcctgttg gggcaagtaa
    14701 tcctcctgat tttgtgtctg gttctattat tgccacagcg tttgtaatat taacgggtca
    14761 agatgtatca gcagcagttg tacttgcagt tcctatagca actctagtat tgcttataga
    14821 taacttctta atgacagtag tacttacatg gggagctcat attgctgacc gttatgcaga
    14881 agaggggaat attgaaggcg tagaacgtgt gcaattatta tttggtatag gaaataaatt
    14941 aatattagca ataatagtag gtataggatt ttcattaggt gttccagtaa tagaaaaaat
    15001 actttcattt ataccatcat atgtaactca tggaatggat gttgcagcag gcgtgattcc
    15061 agcaataggt tttgccatgt tagctagaat gatgcttaat aagaaaacag tagcattttt
    15121 attattagga tttatattgg ttgcttacct aaacataaca gtaacaggag ttgcattgtt
    15181 tggattagca atagctttaa tatatgttaa ctttgtagga gagaaggagg taatagtaga
    15241 tgacaacgag ttctaagaaa ttagagacaa tttcaccaga tagtaaaata acgaggaaag
    15301 acttttggaa atgttttaga aggtcattga cattagattc atcttggaac tacgaacgta
    15361 tgcaaaatat agcatatgca tacatgatgg caccaataat ccgtagatta tataaagatg
    15421 ataaagaaaa gaaatctaag gctttaaaaa gacatttaga atttatgtca gttactcctc
    15481 atataagtac tcttttggta ggtatatctg gagctatgga agaagaaaat gcaaagaata
    15541 aagaatttga tgctaatagt ataaatgctg ttaaatcaag tttaatgggt ccagtttctg
    15601 ggataggaga ttcatttttc tggggaacat taaagcttat agcagctggg gttggtatag
    15661 ctttagcttc tcagggtaac ataatgggac cgattttatt ccttttaata ataaatgttc
    15721 ctcattttat tatacgctat atatgtctag ataaaggatt taagtatgga actcaattct
    15781 ttaaagatgt gagtggttca agtatagttt caaaagttat ggaagcagct tcaatgttag
    15841 ggcttatggt aattggaggt atgactgcat ctaatgtaat gttaaaactt agtgttaatg
    15901 taggtagtgg agaatgggca gagcctattc aaacttactt agaccaaata atgccatgta
    15961 tgcttccagc tatgatattc ggtattatgt attggttatt aggaaaaaag gtaaagacaa
    16021 ctacgatatt aatttcagtt atgattattt gtatagtatt ggctgctatt ggagtagtat
    16081 aaataaaaaa atcccaagtt catttgaacg agggattttt tgactaaatt aaataaaaaa
    16141 gaaagatggt tcataataat ttataagcat ttatgtgata gttataatta gcttattaat
    16201 tagttataaa ctcaaaatta agcactactt tattaaagtt attatattat aatttcgaaa
    16261 tactcacata atctgtttag aaatttatct tctaatgggg aaaaagtttt ctttttagag
    16321 taaatgaaga aaaactttct agtaaaatca acatccttta ttttataaca ttttatttta
    16381 cctgcattga gataatcgat agctgaaatg tacgatataa aggatactcc aagacctaat
    16441 ctaaccattt ccttaatagc ctcatttgat tctacatgag cgattacatt taatttactt
    16501 actggaaagt tatttttact aagtgtgttt agtattaagt ttcttgtacc tgacccttct
    16561 tttctcataa taaaatttag ataagccaat tctcctatat cgatacaatt atttttatta
    16621 tcaattttaa aatctgaagg agtgataaga acaagttcat catctaataa gtctaggtac
    16681 tctatttgag gattattgat tttagaacca actaatccaa agcttattcg ttcatttaaa
    16741 atttctgata tagcatattg agaatcataa tggcttatgg aaaattttac atctggataa
    16801 ctcatagaaa aactcttcat aaaatcaggt aaaatataag tttcaggtat ggaactacat
    16861 gctatatcta tgataccttc tatttttcct gaatattctt ttatatcata aattgcacgt
    16921 ttgcaattat tcaatatata tatagcatgt tcataaagaa tttcaccaga ttttgtgagt
    16981 gtaataactt tgttgcttct atttataaga acagtttcta gctctctctc taaattctgt
    17041 atgtgtgcgc ttacagtagg ttgagttaaa aaaagttctc ttgcagcctt tgaaaaactc
    17101 tgatgttttg caacagcaac aaatacttca agttgtttaa aatccattat ttaatcctcc
    17161 ttaaattatc taaaactatt ataacatatt tgataaatta atagtcaaag gtgattttta
    17221 aaatagaaaa cctttaaata ttcatggagt ttttgattgt ttaggaataa tttggtataa
    17281 tgttgtaagg tacttacaat aaaaatatcg taaattgaat tttcaaaatt caactttaaa
    17341 gtgtttttta ttacagaaaa attacaatta gcttcttaat cttattaaat tcaaataaat
    17401 attatataat tattttataa tataataaat ataattattg aaaaaagaga taactgtaaa
    17461 ttgagatata gataaatact ataaaattta agaaaggagg attgatttat gaagttaaaa
    17521 aggtcattag tttgtgtaac tattttaggt attatacttg ttggatgtca taaagaaaat
    17581 actaaagaga aaaatcaagt agcaagtaaa gcaacacagc aaaaaactat gacaaaagtt
    17641 caaaatgatg ttaatgaaat tatgaataaa gattataagt acattataaa gaatatgggt
    17701 ataccttata atacatttta ttatataaaa cctaaagttt taaaagaatc aaatacaatg
    17761 caagatataa atacatcaag ttatatgaca ttagtttatc caaaatatac tggaaatgat
    17821 gaactagatg gaagtgcttt atatgttgat attaacgaaa ataaagttgt caatgttgaa
    17881 accaattctt tctcatcaca aggtatatca gtcattgatg ctgaatctag tatagtgatt
    17941 gaaaaaagtg accatgaaaa atcggctgta tcactagaaa attttagaca tattgacctg
    18001 ggagagtatg ttggtgttga agattccaga ataaatgaaa ttgtaggaga tgcgaattat
    18061 gatttaacag catataatca tgaaggcagt aaagtagtta agagttatag attgaaagaa
    18121 gataacaaaa tattaaaaaa agaggtactt acaataagta ttgtggataa caaaataaaa
    18181 tctataaaaa ctattgaaag tgataaaatt gtaaaaataa taaaaggaac tttattagaa
    18241 taaaatattc taaagcatat gatgctgttt caagatagaa ggaaatttct agctgggaac
    18301 agttttttta ttagatttat aaaatttgaa ctgcacttat agataactat tagttataat
    18361 taaatataag ataaaagtat ttccaacttt tataatctat aatatattac atagaaatat
    18421 cttaaataaa aaatatattg gggagtattt aaaatgatag aattagaaga tgccataaaa
    18481 attattgaaa aaaatgtttt accaataaac agggtcaaga tagtaaatct tatagatgca
    18541 gtaaataagg tagttgcaga agatatatat tcaacaattg atagtcctcc atttgatagg
    18601 tcaccctatg atggttatgc atataatgca aattctgaaa atagaaattt aaaggttata
    18661 gggactttgt atgcaggaga tgtatttaat agggtattaa ataaaaatga agcagtgaag
    18721 ataatgactg gtggaaagat accacagggt gcaaattgtg taataaaaaa agaagatgta
    18781 acagttatag gtgatatgat aactttaaat ataaaattaa aggaatacga taattacata
    18841 tttaagggtg aggatataaa aaaggggcaa ttgattatta ataaaaatca gaatatagga
    18901 tatgatggta ttggagtatt agcaagtctt ggaatatcca aagtaactgt ttatgatgat
    18961 ttaaaagttg gaattttgaa tacaggtaca gaactacaag atataaatga gactttagag
    19021 aatggtaaaa tatacgatag taatagatat accttatatt caagattatt aaaacttgga
    19081 atacaagcag atacaatatg caattgttct gatgatatat tggatttaga aaaaaaggta
    19141 aagaatatgt tacagagtgt agatttttta attaccacag gtggtgtttc tgttggagat
    19201 aaagatttaa ttccagatgt attcaaaaaa ataggggcaa cagaattatt ttggaagata
    19261 aacattaaac ctggtggagc ttgttatgta ggtacattgg gagaaaagct attatttggt
    19321 ctttctggta atccacatgc agcaatagtg gtatttgata atgtgatagt tcctgttatt
    19381 gagtatttaa catataagaa gggaacacat catataaaag ctatatttaa aggagaattt
    19441 aataaacttt caaataaaga tagatttgta agtggaaaac tatatacaag tgaaggtaaa
    19501 ttgtatgtag agctaaatga taagaaagat gcaaaagcta gactatctgc aactctaaga
    19561 agtaattgta tgattaagat taaaaagaat gagacaataa aagaaaatca attagtggat
    19621 gttgttatta gatagtttta tgaaaggaca caattttatg tataatatat tgtcagttgt
    19681 ctcatattca ggagtaggta aaactacgtt gattgaaggc ataataaaag aattaaataa
    19741 aaaagggtat cgtgttggag ttattaaaca tacctgtcat gattttgata tggatgagga
    19801 aggaaaagat acttataaac atagaaaatc tggggcgaga aaagtctgta taatatcaga
    19861 taaacgagta gcatatattg aagaactaaa agagaagaat ccattatata aaatgattca
    19921 attatatgaa gatatggact tgattatcat agaaggatat aaaaattata gatttaaaag
    19981 attggaagta actagaaaag gtaagtatga ggatatttta tctaataagt ctgatttgat
    20041 aggtattgtc agtgatatag aatataaatt gaatattgaa cagtttaatt taaatgatta
    20101 taaaaatata tcaaagtaca tagagtcttg tataaaaaat gaaattttaa gtataagtaa
    20161 tgttga
//
LOCUS       DAEOSP010000055        19770 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.055, whole genome shotgun sequence.
ACCESSION   DAEOSP010000055 DAEOSP010000000
VERSION     DAEOSP010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19770
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.055"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..441
                     /gene="vanZ1"
                     /locus_tag="KRH54_001627"
     CDS             <1..441
                     /gene="vanZ1"
                     /locus_tag="KRH54_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HBG8579293.1"
                     /translation="ILFKLQFDISSLETMNLRSINLIPFAGSLIINNRVDISEIILNV
                     AIFVPFGIYVCMLKEEWSFIKKVIPIFITSLVFETLQYIFALGASDITDLIGNTLGGI
                     IGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLFLGLVIFANL"
     gene            complement(543..737)
                     /locus_tag="KRH54_001628"
     CDS             complement(543..737)
                     /locus_tag="KRH54_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HBG8579294.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
     gene            complement(1253..1645)
                     /locus_tag="KRH54_001629"
     CDS             complement(1253..1645)
                     /locus_tag="KRH54_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG8579295.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEINTTLYM"
     gene            2212..2574
                     /locus_tag="KRH54_001630"
     CDS             2212..2574
                     /locus_tag="KRH54_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579296.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSKIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            2558..3283
                     /locus_tag="KRH54_001631"
     CDS             2558..3283
                     /locus_tag="KRH54_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579297.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVGGFTEEDEEDEEYEEELKQN
                     IIENIKKNFDEKNLVQVYKYRGV"
     gene            3303..3722
                     /locus_tag="KRH54_001632"
     CDS             3303..3722
                     /locus_tag="KRH54_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579298.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            3712..4236
                     /locus_tag="KRH54_001633"
     CDS             3712..4236
                     /locus_tag="KRH54_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579299.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            4548..5105
                     /gene="efp"
                     /locus_tag="KRH54_001634"
     CDS             4548..5105
                     /gene="efp"
                     /locus_tag="KRH54_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBG8579300.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            5197..5652
                     /locus_tag="KRH54_001635"
     CDS             5197..5652
                     /locus_tag="KRH54_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG8579301.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            5815..6525
                     /locus_tag="KRH54_001636"
     CDS             5815..6525
                     /locus_tag="KRH54_001636"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBG8579302.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            6579..7649
                     /locus_tag="KRH54_001637"
     CDS             6579..7649
                     /locus_tag="KRH54_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG8579303.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            7677..11231
                     /gene="smc"
                     /locus_tag="KRH54_001638"
     CDS             7677..11231
                     /gene="smc"
                     /locus_tag="KRH54_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBG8579304.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEIKDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEGSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKFAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            11258..12538
                     /gene="ftsY"
                     /locus_tag="KRH54_001639"
     CDS             11258..12538
                     /gene="ftsY"
                     /locus_tag="KRH54_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBG8579305.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDIEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            12641..13009
                     /locus_tag="KRH54_001640"
     CDS             12641..13009
                     /locus_tag="KRH54_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBG8579306.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            13006..14358
                     /gene="ffh"
                     /locus_tag="KRH54_001641"
     CDS             13006..14358
                     /gene="ffh"
                     /locus_tag="KRH54_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBG8579307.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPINIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            14396..14668
                     /gene="rpsP"
                     /locus_tag="KRH54_001642"
     CDS             14396..14668
                     /gene="rpsP"
                     /locus_tag="KRH54_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBG8579308.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            14733..14960
                     /locus_tag="KRH54_001643"
     CDS             14733..14960
                     /locus_tag="KRH54_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBG8579309.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            15068..15583
                     /gene="rimM"
                     /locus_tag="KRH54_001644"
     CDS             15068..15583
                     /gene="rimM"
                     /locus_tag="KRH54_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBG8579310.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVAREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            15592..16287
                     /gene="trmD"
                     /locus_tag="KRH54_001645"
     CDS             15592..16287
                     /gene="trmD"
                     /locus_tag="KRH54_001645"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBG8579311.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            16472..16822
                     /gene="rplS"
                     /locus_tag="KRH54_001646"
     CDS             16472..16822
                     /gene="rplS"
                     /locus_tag="KRH54_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBG8579312.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            17143..18039
                     /gene="ylqF"
                     /locus_tag="KRH54_001647"
     CDS             17143..18039
                     /gene="ylqF"
                     /locus_tag="KRH54_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBG8579313.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            18428..19720
                     /gene="brnQ"
                     /locus_tag="KRH54_001648"
     CDS             18428..19720
                     /gene="brnQ"
                     /locus_tag="KRH54_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG8579314.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         8301 a         1858 c         3591 g         6020 t
ORIGIN      
        1 atactattta agttgcaatt tgatatctca tctcttgaaa ccatgaatct tagaagcata
       61 aacttaatac cttttgctgg gtcacttata attaataata gagtagatat atcagagatt
      121 atattaaatg tggctatatt tgtaccattt gggatatatg tgtgtatgct aaaagaagaa
      181 tggagcttta taaaaaaagt tatacctatt tttataacta gtttggtatt tgaaacactt
      241 caatatatat ttgcactagg tgcaagtgat ataacggatt tgataggaaa cacactagga
      301 ggaatcatag gaatagctgt ttttatgcta ttatcgaaaa tatttaagaa taataccata
      361 aaaattatca atgtattggc attgattgtg acaattattg ttgtattatt tttaggatta
      421 gttatttttg caaacttata gaaataagta tatatgttta gcttaaaata gaacgtaaga
      481 tacagttaca tataaaatta tttacacata aaaagcacct atacaggtgc tttcctaaat
      541 tattaaagaa ttattgtatc ttgaaactct tgtatgttca attcttgatt ttctcttgtt
      601 ttttctaata aatctaacgc acaatttata caaactttaa tttctgcatt ggtatttttc
      661 tcataataag ttatatattt atttctatat ttatctattt cttttccaca acgactacac
      721 attacaatta cgctcatatc ttagttcctc catctctaat tattcttaat atattcaata
      781 gtatcacttt tctgaaaata atgattgtta aaactgtatc aaatttagat ttacttttta
      841 ttataatgca ttttaataaa aaaataaagg ttatgggtaa aatatttata gaaaaaagta
      901 tattattgaa tagattataa aattttatta ttttttagca gtgttaaata cacattaata
      961 tctataaagt gacaaaataa aaaatactat ttaaatagat attaaaaaat tgataccaag
     1021 tatttatttg ctatatgaat aacaatttat ttaggaactt tactttaaca ctgtataata
     1081 ataaagtaat tgctaaaaac gtttgatgat ttggatgtat ttttagaaat tagaaaaata
     1141 tatacttact gactaatgat aatcttatat gtattttagt ttgagtgatt taagaaaagt
     1201 gttttgaatg gggattattt tatagagtaa ataaatatat aatagtagaa aattacatat
     1261 aaagagtagt gtttatttca ttaccttctt tatctgttcc gatagggtct tgtagagata
     1321 cttgagtttt attttttttg ttagatctaa tattcattaa aatctcattt tcaatacatc
     1381 tagaagcata tgttgctaat cgagttcctt tatctatgtt ataagtttct atagctttaa
     1441 ttaatcctat tgtacctatg gatattaaat catcctgatc ttccgttgaa ttattatatt
     1501 tctttgctat ataagctact aatctcatgt ttctttcaat taatgtatct tttgcacttt
     1561 tatcgttttc tattttaaat ttagttaaat actcaatctc ttcttcaggg gttaagggtt
     1621 tttcaaaaga tttaagagct gccaataggt cacccccttt tcaacggtca ctttaaatta
     1681 tatgaaggag acttatccaa gttgactgtt atctaataaa ataatatgca tatttttaga
     1741 atttaaaaca tatatttgca aacttttgaa tggaatatga cataaagagt gggaatttaa
     1801 atttaagttt ttgttgtata tatatgaaat aaggcgaaga aattattgat ttataaagta
     1861 taaattggaa ttgaggtgtc aataaatata aaaggaaata gtatttatat atgttttggt
     1921 gttgaagtaa aagattaatg caaagcatta gagtctataa aaatatgaat ataggaggtg
     1981 taattttaca atacttaaaa aagtaaatgt aattaattgt ttgtataaag ttgattacaa
     2041 gcaaaagtat cagtcatagt taatactgtt aagggtgaag gtggtgtgtt aatttatata
     2101 tgtatgggaa gaaattttga ttacaatgaa aaaacttaaa aaagatattt atgaaaaaac
     2161 ttatagttaa atgtttatat ataaaatatt atttaggggt tgtttttatt attgaaaata
     2221 agaaagagtg gttttatatc aattgaatgt ataataagta ttgctatatt atatgtggct
     2281 gtttatttag tttctacatc attgtataat tgttatagtt ttatcagtag aaatatatct
     2341 gacagagaaa tgttaagtac agcaaaaaaa tatatagaag atgagaagta tagaatacaa
     2401 aatagtaagt atgagttaat tgaagataag atagaaaaaa attacataaa tggatatgaa
     2461 attaacagta aaatagagca aattttagat tattatcaat gctatgaaat aaatatagag
     2521 ataaaaaatg aatttaaaaa actgaggttt aatagctatg ttactagaaa ataaaggctc
     2581 tatattaata ataacattga taatattttc tattataagt actgtgtgca ttatgtgtat
     2641 aggtttaatt tatacacata ataatatgtt tgagttagga tacaaggaca ttcaactaaa
     2701 agaaatttct ttaagtggaa tagaaatatc agtaagtaat gtactttctt gtgttgagga
     2761 agctattgat gtatctaata atgaagacga atttagagaa tatttcttag gaaataatat
     2821 gattgaatgt ataaaaaaaa taaaagatac atcttatagt agtttagaag aactttcttt
     2881 aaaagtagaa aataatacta tttatgacag aggagatttt tttgaatttg atatagaaag
     2941 taatgttaga gaaaaagaat ttttaaaagc aataaaagta agagtgaaaa tcaaaaatcc
     3001 atggatagga attgacattg aagattatat ggataaatct aataacaata aaattgatga
     3061 caaaacattc aataattata aagcatctga aaataatgaa attctaaatc aatttaaaaa
     3121 tgaaagtgaa aatataagga atcaagaggt tggtggattt acagaagaag atgaagaaga
     3181 tgaagaatat gaagaagaac ttaaacaaaa cataattgaa aatataaaga agaattttga
     3241 tgaaaaaaac ctagtacagg tatataagta taggggtgta taactttgaa aaaagaagga
     3301 atgtgactgt aatagaatta atagtatcta tcagtataat actcttgata gttacattat
     3361 cttttccaaa agataatctt gaaaatcata aaataaattt atttgcacgt caactttgtt
     3421 cagatataag gtatgttagg cgaattaata tgtttagcaa ctacaataca tatatatatt
     3481 atataaatca aggtaaacac gaaggttatg tattgagaaa agatggtaaa aacattaaat
     3541 caataatgct acctaaaaat gcaaaaatag ctaggagtat tgaaataata aagtttaaat
     3601 ctgatgggtc gcctcaaaag gcgtctacaa tagagatata taataataga ttaagaaaaa
     3661 ctataacaat aacacctgta agtggaagag tacttctgaa agagggaaaa tatgaaacat
     3721 aaatatggtt acttgctttt ggaaagtgtc gtatcattgt cttcaatggt gataattata
     3781 ctagttttat attctatatt tttgtctaca ataaacttaa agttaaaagt agaagataag
     3841 atagaactac aacaacaaag tcttgaaatc ataaaatcta tggaaggaat tattagcaat
     3901 tctatgggaa taatgaatgt atctaattat gaagaaacat ttaaaaagac aacatctata
     3961 aaatgtagat atttagatga aaataataat gaagaaggca taagtaataa agaaattata
     4021 ttgaatgaaa gacgaaataa gttatttgta aattctttaa atggcgaaag tagtcaagca
     4081 ggtggttatg aaattggaga ttatgtagat gagatgtatg tatcaattac taataatggt
     4141 caatatgtta atattaaatt gaaattatca aaacgtagtc aaaaatatga gactgatttc
     4201 aaaataaaag tatggaattt tagtgaaagt atatagaaat acataaattt aagagaaaga
     4261 agtgacatca ataagtcata taaaatataa ttattgagaa ctatataact aaataatatc
     4321 aatatgtaaa gtgtatagat taagaatata gaggataata ataaaaatat agaataagta
     4381 atttgccaaa tacttgttta tattgtataa cttatgatat tatgatatta gtaaaaattt
     4441 gtaatcctga cttgacagaa ttcaagtaag agatataatt ttaaaggcat agtaatgtaa
     4501 aatttactaa aaaataagaa atttatttta atatggagga acaaaaaatg gtatcagcag
     4561 gtgattttag aaaaggtgtt acatttgaaa aagatggaca accatgttta gtagttgatt
     4621 ttcaacacgt taagccaggt aaaggagctg ctttcgttag aactaaatac aaaaacttaa
     4681 aaacaggagc tataagagaa gaaagtttca atccaagtga aaaattccct aaagctgtta
     4741 tagatacaag acaaatgcaa tatctataca atgatggtga gttatattat tttatggatc
     4801 aagaaaattt tgaacaaata ccattaaact atgagcaagt tgaagatgca attaagttct
     4861 taaaagaaaa tgaagttgct acaataagat tttaccaagg acaaccattc caagtggaag
     4921 cgccaaactt tgcagagcta gaagttacag atacagagcc aggtataaaa ggtgatacag
     4981 caagtaatgt aactaaagca gctacagttg aaactggagc agttgttcaa gttccattat
     5041 ttataaatac tggagataaa gtaaagatag atactagaac aggtgaatat ttatcaagag
     5101 tataaaaatt tatgtatata aaagcttata tgtcttaata atataagata tatgagcttt
     5161 tagtttatat ataaaaaaga aagcgagggg tgttacatga aacatttata tgaagaagtt
     5221 gcatacttaa aaggcctagc agaaggacta gaaataagtg ctgaaagtaa agaagggaaa
     5281 atgatacata aaatagttga tgcattagaa gtatttgcag atgctattgt aacattggat
     5341 gaagaacaag aagaacttca agattttgtt gaatctatag atgaagattt agcagattta
     5401 gaagaagata ttgctgacat ggaagaagat ctttatgaag aagatgacga tgaggatgat
     5461 gaggacttca gctatataga aatggaatgc ccaaattgtg gagagttagt agaaatagac
     5521 gaagatttgt tatatgatga tgaagtagat gttgtttgtc ctgattgtaa ggctgtgata
     5581 ttatcatcag aagatgattg tgatgatgat tgtacatgtg gtggttgttc aagttgtgat
     5641 gatagagagt aaaaattcat aagaattaaa actgcctatg atgcctatga gatattttgc
     5701 ttcataggta gttttatttt tatgaggaag ggtattatgt attttggttt tgcaatactt
     5761 ggacaaaaat agtacactag aatttataga ataggatttt ggagggatgt aaatatgaaa
     5821 ataagtaaaa aattactaga taatatacaa agatttgaaa atgttataaa ttataaattt
     5881 aaaaataaag aatatatact agaagctctt actcatagtt catattctaa tgaaaataaa
     5941 aaatataatt ttaacgaaag attggaattt ttaggggatt cggttttagg catagtgata
     6001 agtgattatt tatttaatga agaagctaat ctgcccgaag gggaactaac taaacttaga
     6061 gcaaatatag tttgtgaaga ttcattaagt gaagtagcta atgatataaa tttaggtatt
     6121 cacatgttat taggtagagg agaagaagca acaggaggaa gacataggac ttcaatatta
     6181 gcagacgcat ttgaagctgt aatagctgct atatatcttg atggaggttt tgaaagtgca
     6241 agacagttta tacttcatca tatggaaaat ataatttatg attcaagaaa aggaaatata
     6301 tttagagact acaaaacgca tcttcaagag gtgttacaag ggaatggaga aaataatatc
     6361 tggtacaggc ttatagaaga aaaaggacca gaccataata aaagatttgt aatggaagtt
     6421 ggaattaatg acgatgtact tggtatagga gaaggcaaga gtaaaaaaga agctgaacag
     6481 ttagctgcta aaatagcttt aaagaaaaaa ttatgggaaa aataaatata atatgtaaaa
     6541 atataaaaag aaatatttat attagaagga tgttgtagat gaaaaagaga attataccta
     6601 tatttgttcc tcacaaagga tgtcctcatg attgcatatt ttgtaatcaa aaaaaaataa
     6661 caggtgtgag tactgatgtt actagtgaag atgcaagaaa tataattgag gaatgtttag
     6721 agactataga taaagatgca gatgtagaaa tagcattttt tggtggtagc tttactgcta
     6781 tagatattga tatacaaaaa agtttattat cagttgctaa agaatatgta gaaaaaggtc
     6841 ttgtaaaaga tattagaatg tctacaagac cagattgtat tgatgaagat atattaagta
     6901 tgttaaaaga atataagaca agtataattg aactaggagt acaatcctta gatgaaaaag
     6961 tattattaga tagtataaga ggtcatcaat ctgaaattgt atacaagagt tcaaagatga
     7021 taaaaaatag tggaatcaaa cttggattac agatgatgat tggtcttcca gctgacacag
     7081 aagaaaagtg tatatttaca gcaaaaaaat ttgtagagct aaaaccagat tgtgttagag
     7141 tctatccaac tttggtagta aaagatacag gtcttgaaaa gttaatggaa caaaataaat
     7201 ataatccatt tactcttgaa gagagtgtaa atatagtaaa aaaagttctt gtactgtttt
     7261 atgttaataa tatcaatgta ataagagttg ggcttcaagc tactgatgat atacaaattg
     7321 gaaaagctgt tttagcagga ccatatcatc ctgcatttag agagttggta gaagcagata
     7381 tgataaaaga ttatttagaa tttgttattt tgcaaaataa aaatataaag caaatgttgg
     7441 tcaaatctaa taaaaagaat atttctaaaa tcataggaaa taaaaaaaca aatgttaaat
     7501 atatggaaga aaaatttggt gtgcttttaa aaacacaaga atcagattta gatataaatc
     7561 aattagaaat tgtgttagat gggaaaagtt taattagtgc taatatgaga gacattcata
     7621 gaaaattgta tgacatttat gacctttagt tttaataatg aagggagaga aaagatttgt
     7681 atttaaaaag actagagtta aagggattta aatcctttcc agtaaaaaca gatattattt
     7741 ttaaagaagg aataacagcc atagtaggac caaatgggag tggaaaaagt aatatatctg
     7801 atgctgtaag atgggtatta ggagaacaga gtattaagag tcttagaggt gataaactcg
     7861 aagatgtaat atttgcaggt acagatacaa aaaaacctat gaattattgt gaagtggctc
     7921 ttactattga taattctgaa aatcaattgg aattagattt tacagaagta actataagaa
     7981 gaagggctta tagaaatggt gaaagtgaat tttttctaaa taataaaagt tgtagattaa
     8041 aagatataaa agaagtcttt ttagatactg gtattggaaa agatggttat tcaatcatag
     8101 aacaaggaaa agtagatgaa atattaagta ataatccttt aagcagaaga aaagtttttg
     8161 atgaagcttg tggcatatca aaatatagat ataaaaaaca ggaagcagaa aggaatttaa
     8221 gtaatacaaa ggaaaattta gaaagaatag atgatgtata tatagaaatt gaaaatcaat
     8281 taaagcctct ttttaatcaa cagacaaagg caaaaaagta cttggaaata agtgaaaaat
     8341 taaagacact tgaagtaaat agctttataa gagaaattga aggaatagaa aaagaattaa
     8401 gtgaggttaa tgaacatcgt aaggttattg aaaaggagct caatgaaaaa gaagaacaaa
     8461 aaaatgttgt agagaaaaag caagaagata ttaataaaga ggtagaggta ttacaagatg
     8521 taattgaaaa atctgtagat tatataaatt caattaaagg agttatatca aaaaaagagt
     8581 cccaaataaa tttaattaag gaaagaataa gaaattttac gaatgaaatt agtagaaaaa
     8641 atttagaaat aaaggatata aaagagaagc taaatgaaaa taagcaatac ataaaagaat
     8701 tagaaagcaa taaattatca ggtagtgaag aactatctac cttgcaggaa aatataaaag
     8761 tattggaagg aagtaaagat aaacaaaaaa ttaagttaga atccctaaat aatgagatag
     8821 aattattgaa agaaagtata attgatatat taaataagaa acaagagttt agtaataagc
     8881 tgtctacttt aaacgcaaat aaagaaaata tgaatatcag agatgaaaat ataaattcag
     8941 agataacaga gttaaacaaa aatattgaaa taaaatcatc tgaattagat actataaata
     9001 aagaatttaa tatgcaaaat gaaaatttaa agaatgtaaa taatagacat aaagaattat
     9061 caattaattt gcaggattca attagtgaac acaataaact agaagatgaa atacagaaaa
     9121 gtaaatataa tttaaatgga tataattcaa agttaaatgt ctatatagat atggaaaatc
     9181 attatgaagg atttaataga ggagtaaaag aagtactaaa aaataaaaat ttaaaaggag
     9241 ttcatggagc attaggacag attataaatg tacctgaaaa gtatgagaaa tctatagaag
     9301 cagctcttgg tgcttatatg caaaatataa taactgataa tgagtttagt gctaagtttg
     9361 caataaacta tctaaaacag aataatttag gtagagtaac atttcttcca ttaaatataa
     9421 ttaaatcaaa taaaataagt ctaggaaatt taaaagcaaa tactaagttc ataggaatag
     9481 caagtgattt aattacattt gatgaaaagt atagaaatat aattgaaaat atacttggaa
     9541 gaacaatact tataaataat atagatgagg gcataaaatt tgcaaaagaa actggtcata
     9601 ggtttaaaat agtaacttta gatggtgaaa tcttaaatcc aggaggttct ttgactgggg
     9661 gaagtctaaa aactaatggg aatatactat cgagaaaaag atacattaat gaatatacag
     9721 aaaagataag taatatcaag aatgaaattt cacatctaga attaaaaaga gaatctttag
     9781 acaaagatgt gaaaaatatc aaaaatgaaa ttgattcaca tgaaagtaaa ataaaagatt
     9841 tagaaaaaag tataataata aaaagtacaa gtataaagaa tgttgagtct gaaattgaat
     9901 cgttaaaagg tagtattact aaattagaaa atgagaagaa tgatttaaat tctaacctaa
     9961 attatacact tgaaaaaagt gatgatgtta gaaaagatat ggaagagtta gatgatttat
    10021 acaataaaaa taaagaaaaa attgatgcat tgaatgagga aataaaaaga tacaatgatt
    10081 tatatgataa agaaaaatct gaatttgatg aattgaactt aagtttagtg aagaaaacag
    10141 aagtttacaa tagtatagtt agagatataa aaagaatatc tggtgaaaat tgtgagctag
    10201 aagaaaaaaa taaacaatta gaagagtcat taaattatga agaacatgaa ataattaaat
    10261 tacaagattc tatactcact gaagaaaaag aaaaagaaaa tttaacaaaa caactagggg
    10321 atagtaatag aaatcttgaa actagaaaaa tagcaaaaga tgatttaaaa aatagttttg
    10381 atgaaattaa taaagaatta aaaactatag atagacagca tatagaactt aaagaaagtt
    10441 tatttaaagt tggtggaaga ttagaaagat taaaaactag tcaagacaca tatataaata
    10501 agttgtttga gcaatatgat atgaccttag tacaagcatt ggaaattaaa gatgaagatt
    10561 tggatataga tagaaaattc ttagaaagtc taaagagaga aataagaagt cttggaaata
    10621 taaatataga ctctataaag gaatatgagg aaataaaaga aagatacgat ttttatagcg
    10681 aacaaaagca agatttagaa gagtctatgg aagagataga aaaacttata catactttag
    10741 aagaaaatat gaaatctgaa tttgaaatta agtttgaaga aataagtaaa aattttaaat
    10801 atgtatataa gagattattt ggtggaggtt gtggagaatt aactatttta gataaagaaa
    10861 atttattaga aagtgatata ctaataacag ctcaaccccc aggtaaaaaa atgaaaaatt
    10921 taaacctatt atctggtgga gagaaagctt taacggcaat aagtatatta tttgctatat
    10981 tgattacaaa gccaacacca ttttgtatat tagatgagat tgaagcgcca cttgatgacg
    11041 caaatatatt tagatttgga gaatttttga aggatttatc taaagagact caatttatat
    11101 cagtaactca taggagagga acaatggaag ctgctgatta tatctacgga gttacaatgc
    11161 aagaaaaagc catttcaaaa gttattagtt taaaattaaa agaagctcag gaaataacag
    11221 atattatata acttagattg acataggagg aataacagtg ttaaaaaaat tatttggttt
    11281 tggaaaagac aaggaaaagg aaatagaaaa aaaagatgct gaagaagaaa tagaagttga
    11341 agacagtgtt gataatttag aaaacttgga agaaactatt ttttcaggac ttgaagaaga
    11401 agtgatagat aaagtggaag atattgaaga aaaaaatgag gaaagtgtca ataatgaagg
    11461 cactgaagaa atcgaaaact ttgaaaaaat agaagttgag atggatagta atggaaaaga
    11521 agtagaaaat atttcaaata atgattctca agaagagata atcgaagaat tagaaaatta
    11581 tgatgaaatt gagtctaaag agatagaaga taaagaagat aagaaagtta acttgtttga
    11641 aagactaaaa caaggattga caaaagcaaa gcaaggaata actgatagaa tagatgaagt
    11701 tttaaaatca tatacaaaaa tagatgaaga attacttgaa gatttagagg aaattttaat
    11761 aacagcagat gttggtgtaa atacaactat ggatataatt gaaagactaa gagataaaat
    11821 aaagcaaaaa ggtattactg aaccaataaa ggtaagagaa gaacttaaat ctatagttga
    11881 agatattctt acaaatgaaa actctacact agatatagaa ccagcaccat gtataatatt
    11941 aatggtgggt gtaaatgggg tcggtaagac tactacaata ggtaaactag caaatagata
    12001 caaaaaagat ggtaaaaaag ttttattagc agcagcagat acatttagag cagcagcgac
    12061 agagcaatta gaaatttggg caaatagaac caatgtagac ataataaaac atcaagaggg
    12121 agcagaccct ggagctgtag tatttgatgc tataaaagct gcaaaagcta gaaaaacaga
    12181 tgtattaata tgtgatacag caggaagatt acacaataag gctaatctta tgaatgaact
    12241 tggaaaagta tttaaaatag tagatagaga atttccagaa gcaaaacgtg aagtacttct
    12301 tgtggtggat gctacaacag gtcaaaatgc agttgttcaa gcaaagacat ttaaagaagt
    12361 tgcagatata acaggtatag tacttacaaa acttgatgga actgcaaaag gtggagtagt
    12421 ccttgcagta aaatcagaag tagatgtacc tgtaaaactt attggagttg gagaaagtgt
    12481 agaagaccta caagatttca atgcaaaatc attttcagat gcattatttg gtaactaatg
    12541 ttcttttaaa aataatggtt gacaagagtg ttgttatgat ataaaataca atagtgtaaa
    12601 gtaaataacc tttacagtat actgctagga agtgtaagat atgaatatag aaaaaatggt
    12661 cgaaattgga ttgttatttg aacagtataa agaattatta actgacaagc agaaagaaat
    12721 agttgctcta tactatgaag aagattattc tttaggtgaa attagtgaaa atctgaatgt
    12781 atcaagacaa ggtgtttatg ataccttaaa gcgttcagaa aaaatactaa gagattatga
    12841 agaaaagtta cacttagtat caaagattca agagcaggaa aaaaatatta agattataaa
    12901 agataaaatt attgacatta aagaagattt attgcacaat agagattgtg ctaatttaat
    12961 ccctaagcta gaaaatatag aagatgtatg tagggagatg ataaaatgat atttgaagga
    13021 ttgtctgata aactacaagg ggcatttagt aagttaaaat ctaaaggaaa acttacagaa
    13081 gtagatgtaa aaaatgctat gagagaagtt aaacttgctc ttcttgaggc agatgttaac
    13141 tttaaagtag ttaaagattt cataaaaaaa gttcaagaga gatgtgttgg gcaagaagtt
    13201 atgcaaagct taactccagc tcagcatgta atcaaaattg taaatgaaga gcttacaagt
    13261 ttaatgggag atgtacaaag taaagtaatg atttcctcaa agcctcctac aattttaatg
    13321 atggttggat tgcaaggtgc aggtaagact actacagcag gaaaacttgg aggatatttt
    13381 aaaaagcaag gtaaaaaacc tctattagta gcttgtgata tttatagacc agcagctata
    13441 aagcaacttc aagttgttgg agaaaaattg gatatacctg tattcaatat gggagataaa
    13501 gaaaatccaa taaatattgc gaaagcaggt ttaagccatg cgattaaaaa tgctaatgat
    13561 ttagttatta tagatacagc tggtagactt catatagatg aagtacttat ggatgagtta
    13621 aagtctatta aatcagaagt taagccacat gagatacttt tggttgtaga ttctatgaca
    13681 ggtcaagatg ctgtaaatgt tgcagaaagc ttcaatgagg cacttggtgt tgatggagta
    13741 gtattaacta aattggatgg agatactaga ggtggtgctg cactttcaat aagagcagtt
    13801 acacaaaagc ctataaaatt tatggggatg ggagaaaagc tagatgatat agaacctttc
    13861 cacccagata gaatggcatc aagaatatta ggtatgggag atgtcttaag tttaatagaa
    13921 aaagcacaag aaaatataga cttagagaaa gctaaagaat tagagcaaaa aattaaaaaa
    13981 caagagcttg attttgaaga tttcttacaa caaatggaac aaatacaaaa tatgggacct
    14041 cttgacaaga tacttggtat gatacctggt atgggaaatg taaaagacca acttggagat
    14101 gtagatttga atggcaaaga aatgaagaga acaaaagcca taattcagtc aatgactaca
    14161 tatgagagaa gaaacccagg agtattaaat gcttcaagaa agaaaagaat agctaaaggc
    14221 agtggaacta gtgtacaaga tgtaaataga cttataaaac aacttaatga aatgaaaaaa
    14281 atgatgaaga tgtttgctgg ttcacaaaag agtatgaaga aaaggggcgg cttaggcggt
    14341 ttaccttttt ttaaataaaa tataaaaaag taaaaattat tcggaggtga ctgtaatggc
    14401 agttaaaata agattaaaaa gaatgggagc taacaaaaaa cctttctaca gaatagtagt
    14461 agctgattca agagctccaa gagatggaaa atttatagaa gaaataggat actataatcc
    14521 aatttctgaa cctaaacaag taagaataaa tgatgaaaaa gctataaaat ggttagctac
    14581 tggtgctcag ccaacagaag tagttaaaaa attacttgta aaaaacggag taatagaaaa
    14641 attcgaagct tctaaacaag caaaataatt ttttcttggc aattagttta agtaagggct
    14701 cattgctaag gttactttag gagatgaaat atatgaaaga gctagttgta gatatagcta
    14761 aggctctagt tgataatcct gactcagttg ttgttgagga atttgaagat aatgatggta
    14821 tcgtcttaaa gcttacagtt gctcaagagg atatgggtaa ggttattggg aagcagggaa
    14881 gaatagctaa agctataaga actgtcgtaa gatctgttgc aaatagagaa aatataaaag
    14941 tttctcttga gatagtataa aataggagga ttaggtataa tgcctagtcc tttttatata
    15001 aatgcgtagt ttaagatttt atgatgatat gaccacataa gattaaattt tttaagaagg
    15061 tgataacatg aaagaaaaat taactcattt taaggtaggt caaatagtaa atacacaagg
    15121 tttaaaaggt gaagtaagag tttatccgct tactgatgat atagagaggt ttgatgaatt
    15181 aaaagatttc tatctgggaa aagatttaaa taataaattg gctgttgaaa aagtaagata
    15241 caaaggttct atggttataa tgaagataaa aggcatagac agcatagaga aagcagaaaa
    15301 actaaaaaat aaatttatgt atgtagctag agaagatagc agagagcttg aagaaggcga
    15361 attttttata gcagatatga ttggaatgga agttttaact gtagatggaa aatatgtagg
    15421 aatactagaa gatgttcttc aatactcagc aaatgatgtc tatgtagtaa aaggtgagga
    15481 agataaagaa tttatgatac cagctataaa aaaatttgta cctacaatag atattgatga
    15541 aagaaaaatg attatagacc ctattaaggg aatgatagat taggtggtaa tatgagattt
    15601 catataatga cactttttcc agaaatattt aattcgtaca tggatgaaag tataatgaag
    15661 agagcagttg aaaaaggtat aattgaggtt catatatata atataaggga tttttctaat
    15721 aataaacata aaaaagtaga tgattatcca tttggaggag gagcggggat ggtaatgact
    15781 cctcaaccta tatatgacac atataagcat ataataacaa ctcataatat aaataaccca
    15841 agtgtaattt atcttactcc aaaaggtaag gtatacaatc aatctatggc aaagcagatg
    15901 tctttaaaag aagatataat actactatgt ggtcattatg aaggaataga tgagagaatc
    15961 attgatttaa tagttactga tgaaatttca attggagatt atgtattaac tgggggagag
    16021 ctcccagcac ttataatgat agattctata tcgagattaa taccaggtgt attaaaccaa
    16081 gaggaatcat ttgaagaaga atcatttaaa gataacttat tggaatatcc tcattacact
    16141 agacctagag attttgaagg attaaaagta ccagaagtgc ttttatcagg gaatcataag
    16201 aagatagatg agtggagaag agaagaatct ataaggataa ctaaagaaag acgatttgat
    16261 ttatataaaa agtctaatga aaaataaaaa tataatgaaa aattaatata tatgtataca
    16321 ataaagtacc tataaatgga attaaaaaca taaaagtgct tgtaataact attgaagtat
    16381 agtataatat aaaatgttga aaatacgggc gttcctctgt ttacactggt cgggtagtta
    16441 agaacgtcta tgatatctag gaggaagaaa gatgaatgaa atgctaagag caattgagca
    16501 agaacaatta aaaaatgaag ttcctaattt tggacctggg gacacagtta aagtacacgt
    16561 tagaattata gaaggaaaaa gagaaagaat acaagtattc gaaggtgtag tattaaagag
    16621 acaaggtgga ggagcaagag aaactttcac agttagaaaa atgtctttca atgttggtgt
    16681 agaaagaact ttcccagttc actctccaaa aatagaaaaa atagaagtta ctagaaaagg
    16741 taaagtaaga agagctaaac taaactactt aagaggtaga gtaggtaaag ctgctaagat
    16801 aaaagaagct agaaataaat aattaaaaac tttacttata aatggggggc tgatttatca
    16861 gtcctctttc tattttaatg aaaattacag caaataatta tactagaaat aaatagaaat
    16921 atttgttgaa aatatacatt atttatctat gaatttatag cctttaaaat gaaaaataaa
    16981 attggtaaat attgcattat attttgtaac aatattaaaa ataagagaac agtattatgt
    17041 aaagtatgac gttaaaaaga tgacaatatt atagtttgtt aagatagatt ttttaagtgg
    17101 aattaggata taataatata atattaataa ggagtgatac atgtgtcaaa tgaatacgaa
    17161 gagtatctta tgaatgacaa tctacatata aactggtatc caggtcatat gaaaaaaact
    17221 aaggagttag taaaaaataa cttaaaattg attgatgtag taattgaact tttggatgct
    17281 agaatcccgt ttagtagtaa aaatccagac atagacaggc ttgtaggaga taagccaaga
    17341 gttgtcgtac ttaataagag tgatatggca gatagggata aattgaatca atggattgaa
    17401 tactataaga aaattaatat caaagctata ccagtagata caataaaagg cgttggtata
    17461 aataagataa tcgaagaatg taaaaatgta actagagaaa aaatgagttc tttaaaagac
    17521 aaagggagaa aagaaagagc tatacgaata atgatagttg gagttccaaa cgttggtaaa
    17581 tcgtctctta taaacaaatt aacaggtaga aaaagcactc aaactggaga taagccagga
    17641 gtaacgaaag ggaaacaatg ggttagatta aagggaaatc ttgaactttt agatacacct
    17701 ggaatacttt ggcctaagtt tgaagaccaa gaagttgcat taaatttagc atttagtaga
    17761 gctataaagg atgagatttt agatactgaa actttggctt taagacttat agagaaactt
    17821 atgaaaatag aacctgagaa gttgaaagcc agatataagt tagattgttt aggagaaact
    17881 ccaattgaaa ctatggatat gattggccat aaaagagggt ttataacagg taaaaaagaa
    17941 ctagattata cacgcatagc cacaactgta ttaaatgaat ttagagatgg aaaaatagga
    18001 aatataacat tggaagttcc agaaaacgtt aaaagataga ttttaaaaca aaatcaaaaa
    18061 tttaatgtta aaaatttaaa aaagcctttt agcaaaggct tttttatttt tttgaaatat
    18121 agacaaatat ctccatataa aagtataaat taggttagaa aacctttaaa aatattaatt
    18181 taagacaaat atacctaata ttttgaaaat taaaatgagt gtgattattt aaaattttag
    18241 catattgact tgcttgtttc gtataatata taataagcac aaataaatac aatatattaa
    18301 aaatttaatt tatttaattt tattaattta ataataagtt cttattgttt aattcaactt
    18361 acttatgcaa gcgtaaacaa tcgagacatt ttattaattt aataaggtta aaaaggggga
    18421 ttttataatg ggtaagacca aggatgttat agtatttgga tttgcgttat ttgcaatgtt
    18481 ctttggagca ggtaacttaa tatttccacc ttacttggga ataattacag gtccagaatg
    18541 gttaattgcc ttcttaggat ttacgtttgc tgatgctgga ttggcacttc tagcagtaat
    18601 ggctacagct aaattcgatg gaaacgtagt tgaaatgttt aaaagatgtg gtatgaagtt
    18661 agggattctt ataggttgtg cagatatttt atgtataggc ccattcttag ctataccaag
    18721 aacaggtgca acaacttatg aaatgggtat aatgcctttg tttggaactt caattccagt
    18781 gttattattc tgtatacttt tttttgcgat ttcatatgta ctaacaataa gaccttctaa
    18841 ggtcgtagat atagttggac agtttttaac tccagctctt ctaatagctt tagcatttat
    18901 aattataaaa ggtattatat cacctcttgg agatatagta gataaaccta tgatacctaa
    18961 tgtatttgca gaaggtatag gtcaaggata tcagacaatg gatgcttttg cagcaatagc
    19021 acttgcatca gtgctaatag tatctttaaa tgataaagga tattctacta ttagtgacaa
    19081 gttgaaaatg attggtaagg cgggtgttct tgcttgtgga ggattagctc tagtttatgg
    19141 aggattatgt ttccttgggg caactgtatc aacaatgtat ggaacagatg cagtacaatc
    19201 acaagtaata gtaaatataa ctgaaggatt gcttggaaat gttgggaaag cgattttagc
    19261 agtagtagta tcattagcat gtttaactac atctataggt cttacatctg ctacaggtca
    19321 atatttctct agacttacaa aaggtaaatt gagttatgaa aaaatagtat tagctgtatc
    19381 tgtattcagt gcagtagtag catcttttgg tgttggaact ataataaaga tagcatctcc
    19441 tatactaagt atagtttatc caccatcaat tgttttaata attttagctt tcttcaatga
    19501 aaaaattaag aatgataatg tatacaaagg agcagtatat atgtcattgc ttgtaagtat
    19561 actaacagtt atatctagtt atggagtagc tgttccagta gtaaattctt tacctcttaa
    19621 ttcattagga tttaactggg tggtgccagt tataatagct ggaataattg gtaattttat
    19681 accatcaaag agtcaatcta atacacttgg aacaaactaa gttttgttta attaaaatat
    19741 aatcaattgt aaataaatga gttaactaaa
//
LOCUS       DAEOSP010000056        19467 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.056, whole genome shotgun sequence.
ACCESSION   DAEOSP010000056 DAEOSP010000000
VERSION     DAEOSP010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19467
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.056"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..720
                     /locus_tag="KRH54_001649"
     CDS             <1..720
                     /locus_tag="KRH54_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG8579315.1"
                     /translation="YVSAYHKDNLEAKSANKVYKIGGGLTSKVMSSIASSLSKHNTTP
                     TEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKDYNLNTSLATTEYLRSKGFNVIMTRD
                     TDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNKQGHGVEVFYKLKDKNGGITKTVATN
                     ILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVLRSNDMPAVLVECAFLDNENDMSLIN
                     SSAKVKEMGTQIGKGIEDSLK"
     gene            962..2875
                     /locus_tag="KRH54_001650"
     CDS             962..2875
                     /locus_tag="KRH54_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579316.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERSLRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYREILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            2868..5087
                     /locus_tag="KRH54_001651"
     CDS             2868..5087
                     /locus_tag="KRH54_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG8579317.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            5196..6026
                     /locus_tag="KRH54_001652"
     CDS             5196..6026
                     /locus_tag="KRH54_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBG8579318.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            6016..9483
                     /gene="addB"
                     /locus_tag="KRH54_001653"
     CDS             6016..9483
                     /gene="addB"
                     /locus_tag="KRH54_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBG8579319.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINIIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEVYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            9483..13310
                     /gene="addA"
                     /locus_tag="KRH54_001654"
     CDS             9483..13310
                     /gene="addA"
                     /locus_tag="KRH54_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBG8579320.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEN
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            13356..14567
                     /locus_tag="KRH54_001655"
     CDS             13356..14567
                     /locus_tag="KRH54_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBG8579321.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            14554..18081
                     /locus_tag="KRH54_001656"
     CDS             14554..18081
                     /locus_tag="KRH54_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG8579322.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNKIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKLDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKIKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            18212..19465
                     /locus_tag="KRH54_001657"
     CDS             18212..19465
                     /locus_tag="KRH54_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG8579323.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
BASE COUNT         8452 a         1787 c         3468 g         5760 t
ORIGIN      
        1 tatgtatctg cataccataa agataattta gaagctaaat cagctaataa ggtatacaaa
       61 ataggaggag gattgacttc taaggtaatg agctctatag catcatcatt atctaaacac
      121 aatacgactc caacagaacc aggaaatagt ggtggcaaga cagttatgat tgacccaggg
      181 catggtggtt cagcacctgg aaattcatct ggaggaatga ttgaaaaaga ttacaattta
      241 aatacttcac ttgcaacaac tgaatattta cgttcaaagg gattcaatgt aataatgaca
      301 agagacacag ataagacttt atctcttgga aatagaactg ctctatctaa ttcattgaaa
      361 ccagatttat ttacaagtat acattataat ggctcaacta ataaacaagg tcatggtgta
      421 gaagtatttt ataagcttaa agataaaaat ggagggatta ctaaaactgt agctaccaat
      481 atattaaata gaattttaga gaaatttaaa cttacaaata gaggtataaa aacaagagta
      541 cttcctagtg attctacaaa agattattta tacgttttaa gaagtaatga tatgccagct
      601 gtacttgtag aatgtgcatt tttggataat gaaaatgata tgagtttaat aaactcatct
      661 gcaaaagtaa aagaaatggg tacacaaata ggtaaaggaa tagaagattc attaaaataa
      721 atggaaataa aaatatttaa aaacttaatg taaatttata gttgtaaatt tatactaaag
      781 tcaaaaaatg aaatataagt gtatcaatag taagactgta tgtacatttg cacatgtgaa
      841 tgcaagtata tatacagtct ttttttgtag ttaaatgcaa taatttgatt gtgttttgta
      901 aaatacacaa tatgatataa ttagataata tacacttaat ttaattgagg aaggtgatta
      961 tatggaatat ttatttgaaa aaagtagttt aaaagaatat ggaaatgaag ctttagataa
     1021 atatagagaa ttattagata aaactggtaa taattcaagg tgtattcttt tgttagtacc
     1081 aaacaataac acaaagatac gatatgaaag gtctctaaga ttaaattata gtgaagaact
     1141 gaaaataaca acatatataa gttttgtgaa gaaagaatta gttaaatact ggccactaat
     1201 aatagaaaaa tgtgatgaaa taagtacaaa ggttgtatct ccaacattta tatcaaatag
     1261 tttaagtgaa tatttaatag tgcaaaaagt taaggaaaaa aggatacaag aaggatattt
     1321 tgaagatatt acaggaacca ataaatccat agcaaatagt ataatgacta acataaataa
     1381 gtctgcattt aatttaattg attttgattt tattggagaa aaaatatatt catcaaagaa
     1441 aaataaagat agtatatata gattttcata cactcaaatg gatgaaatta tatcatatta
     1501 tataaattcc ttactatcaa attcaatttt agataatgca ttaagcatat atttatacaa
     1561 tcaataccta cttaatgatg atttttattt aaaaaactta tttacagaag tgagatatat
     1621 aattgtggat agccttgaaa gctcaagtaa tgcagaagtt gattttataa cagaagcatt
     1681 ggataataca ttacaaagtt atatatattt tgattatagt agggattatt ctgtatttaa
     1741 taatgtggat atagactata taaatgagaa aataatatca aaaattaagc ctaaagaaga
     1801 aaatattaaa aagaaggaga ttagaataga agatttatac ttattacctg caaatataga
     1861 attaaatcaa tcaagccaat tatataatga aatgttagat ttaataagtg aaaaagttat
     1921 atctcttata gaagaagatg tatctccaag agatatagca attatatctc ctataaacaa
     1981 tagtatattg gagcatcaaa ttagagattc tttgatagaa aaaaatatag atgtttttag
     2041 tactaagaag gataaaaaag ctgtggatta tccatatggg aatgcattgg tagtggctac
     2101 atgtatattt tatggatatt tagattttat aaaagatgat gaatacttaa gctttttaga
     2161 aactctttta gaaataaata ggataaaagc atttaagatt tttaatgaaa caagacattt
     2221 aagtattgat aatatagatg aacatagtca gtatcgtgag attttacagt atattgaaga
     2281 aaaaaagaag agtgatataa aaatacatga atttttaact caattttaca tagataaaat
     2341 gttaaattta aaagaaggta aaaagaatgt tgggttatgt aaaaagataa tagctgaaag
     2401 tgagtctttt agtgaaagtt taagtctact tgggatgaaa aaagaaaaga tatttataga
     2461 agctttaaaa agtacaataa acgattatta ctctgttgtc gatatagaag agttgaaatc
     2521 caaagacaaa atagtaataa ctactcctta ttcatatata tcatcaaata tagatagaag
     2581 tatacaaata tgggtagata taggaagtaa tgcttggaat atgaaaatag aaaaagatat
     2641 aagtaattta attgtactta gaaaatcttt tgaagaaaaa aagatttata caaatgaaat
     2701 ggaagaatat tataaaaaat actatctata caatacagta tataatttgt tattaaatgc
     2761 aaagaaaatc tatgcattta aaagtgaata tgcgattaat ggttatatcc aagagagtat
     2821 attatatagt atacttttaa aaatctcaca taaaggagat aaaaattatg attaatataa
     2881 attatagaga agaccagata ccaataataa attacgataa tggtactatg gcagtaccag
     2941 cagtaccagg tgctggaaaa acatttataa taacaaattt agtagctaaa ctattacttg
     3001 agcagaaaca taagggtggt aaaatattaa ttcttacata tatgaatagt gctgtaaata
     3061 attttaaagg tagaataaga aagattcttg aagagaataa aatagatgat actaattcgt
     3121 atgaagtaat gactatacac agtttggctg taaaaataat aaaggaaaag ccagaagttg
     3181 taatgttaag tgaggatttt aacatagctg acgatttgca aaaaagtatt atattaaatg
     3241 aatgtataag tagatttaga tttgaaggtg gagaaagagc ttttagatgg tttttaaagg
     3301 agcaaaaaga tgaacgctgg aaagaaataa ctcttgaagc ctgggaaaga ggtttttttg
     3361 aatttgcagg aaatgcaata agtgaactta agtataaggg aatttctcca gataaacttg
     3421 aagaaatatt gtcaagagga cacaaaggaa tgcttaaaat tattttacca atatataaat
     3481 tgtatgatag aaaattaaag caaaatggat tgctagatta tgatgatata ttaattttag
     3541 cagaaaaaac tttaagctta gacgaagggc ttagaaagaa gtttcaaact agatacaaat
     3601 atatatttga agatgagtgc caggactcaa atgaaattca aggtaatata ataaaaatta
     3661 tatctagcga aaataaaaac ctagttagag ttggagatat aaatcagagt ataacaggaa
     3721 cattttcatc atcagaccct aaatttttta aagaatttat aaatagtgca gatttctgtt
     3781 ataggatgga tatgtcaaat cgaagttcaa aagatatact tgatttagca aacactcttg
     3841 tcaagtatgt tacaaaggaa tttagacaga aagaatgtag acatgctctt gaagatatgc
     3901 aaataaaaac tgtgccaaat ggaatgggat ataaagaaaa ccctaatcca gaacattata
     3961 atataaatgt aaagtggtat gagagttgga aaattgaaat agagcaaact gctagatacg
     4021 ttaagggtat aaaaaataaa tatccagata aaagtatagg tatattggtt ccatttaatg
     4081 aacaagtgac acaagttgct aaagaactta gagagaataa tctaaccttt gaagaattag
     4141 gaccaaattc tttgaataaa aggaaggtta taaacaatat agcatatatt attgatttta
     4201 tgttgcattg tgatgatgta gaaaagttaa tagttgtact taataaagtt tttatagata
     4261 ctgataatga agaagaaata aagtattttt tagaagaatt aaaaaactat tctaccgaag
     4321 aaattattta taattttgaa tttagtaagt tagataatga tttaatagaa tttaaagaag
     4381 aaagtgatat atacttaagt tttaaaagag gaatagatat tataaaacag atattagaat
     4441 atccaataat aaggttagac ctcttagttt tgtttattgg gaaaaaactt aatttagaaa
     4501 aagaagataa agctgtactg gattatatat ctttttatat aaagtatcta gtatcagaaa
     4561 atataaatat ggatttagaa gatgtatata atttattgtt tgatgtaaag aacagggtgt
     4621 ttaatcatat aatagatgtg gtatatgaga taaatggata tgaaccagag cccggtagta
     4681 taacactatg taactatcat aagtcaaagg ggatggaatg ggattgtgtg tttttactag
     4741 gtcttgttga atataatttc cctgacaata ttaatcaaaa gtttcagagt gataaatggt
     4801 atctaaaaga aaaatataaa aaccctatgg caattattaa atcagaagtt gaagctatat
     4861 taaaaggaag tatttcaaca gattatgctc ataaaacgaa gattgattct ataaatgaaa
     4921 agattagact tttatatgta gggattacta gagcaaagga aatgttagta ctttcaggaa
     4981 gtgcttatag agatgaaagt gatataggga ataagagaaa agagcaaaaa ccatgtatat
     5041 atttaagcag acttaatcaa catattattg aaaaaaggtc aaattaatat ataataagtt
     5101 attaaaagat tgcttgaaat atatctaaaa ggggataaaa caatatgtac agtaagtaac
     5161 tactaaaaaa taaagttaag agagaagtga tatgagtgaa cgataaatta aaatattttt
     5221 cttatagtca aaattcaatt aacacatata aatcatgtcc attgaaattt aaatataaat
     5281 atattgatag aataaattgg aaaaatgatg atgttggtag tagagagtat tatgaaactt
     5341 taaaaacagg aagagatttt catttgattt gtgaaagata ttttagcaat atacctcttg
     5401 gaatctattt taatgaggat gataaaaata gtaaaaagtt tttaaagtgg atggaaaata
     5461 taaaaaaagt tgttcctatt gaaaaaggaa aaacgtattt gccagaatat gaagtaagga
     5521 tgacattaaa tggggatata atacaggcaa agtatgattt agttgtaatt ggaaataaca
     5581 atatagaaat atgggattgg aaaaccgagt ctaaaaagat tgactataaa cacgtagaaa
     5641 atagaataca aactattgtt tatatgtttt tagcaaaaga agtaatacct aaagttttaa
     5701 aaatggacat aaatgttaaa gatataaata tgagatatta tcaacctgaa tttgatgact
     5761 taccaattac cattttatat gatgaagaaa aacatgaagc aaatagaaat aaaattcaaa
     5821 attatataag tatgataaaa aacactaatt atgaagaaca tacatacgaa gacaatctat
     5881 acaatgatat agaaaaggta tataataaag aagaacgtat gtgctataga aataaaaagc
     5941 attgcaagta ttgtgaattt aataagttat gtaatggcaa agaaatagat tatagtattt
     6001 tggaggcgga aatttatggg acttaggttt gtattaggta gaagtggaag tggaaaatcc
     6061 acctatatac tagatgagat taaaaaagaa gctcaaaaaa atgaaacgac atcaattata
     6121 cttttagttc cagaacagta cacatttgag gctgaaaata gagtaagtaa actattttta
     6181 ggaaaagaaa aagataagta tctaagagtt agggtactta gctttaagac actaagtaat
     6241 atagtttttt ctcaagtagg aggattgaca gatgtaaata taaattctag tggaaaagca
     6301 atgatggtat atagagctat tgaagatgta agtgaagaat taaatgtgtt ttcaaaatca
     6361 aaatcgcagt ctggttttgt aagttcaatt acagatatga tatcagaaat gaaacaatac
     6421 aatataagcc ctgaaatgtt agaaaatata tctggagaat tggataatga aactttaagt
     6481 ttaaaattaa aagatataag taaaatatat aattcttttg aagggaaatt gcatgaaaat
     6541 tatgtagatg ctcaagatat gttaacttca ttggcaagta aaattgaatt aagcagttat
     6601 ttagatggag cttgtgttta tatagatgaa tttacaggtt ttacaccaaa tcagtacaat
     6661 gtcataaaga gtatattaaa taagtctaaa agtgttaata tctctttgac tgtagatgat
     6721 atcaactata ttgggtacag taaatctgat atgttttcta gaacgaaatt tacttattca
     6781 aaattgactc aattgtgcaa cgaagaaggc ataaaaatat tgccacaagt aaatttaaac
     6841 acaggtgtta taaaaagatt tgaaaaagtt aaggaattac agcacttaga aagattttat
     6901 aatgcttatc catataaaat atattcaaat ccaactgaaa atataaagat aaaagagttt
     6961 aacaatttat attcagaggt ggaagagata gccagagaaa ttgtacattt agttagagat
     7021 aagaatgtca gatatagaga tataaccatt gctacaagag atttaaatag atatgatttt
     7081 ttagtacact ctatatttaa tgaatataat ataccaaact ttatagataa gaagagagag
     7141 gcaaaaagta atccaatagt tattttaatt atttcagctt tagaaatgaa gaatagacga
     7201 tatgggtatg aaactatgtt taggtattta aaaagtggac ttataggaat tgataatgat
     7261 gatataaatt tactagaaaa ttatgtgttg gctaatggga ttaaaggtaa aaaatggttt
     7321 gatgaaaaat gggattatag aataactcaa agtctttcag gtcaagaaag tgaatttgaa
     7381 ctggaattaa aagagaaaat aaatgaaata aaaaatagag tattagagcc tatagtaatt
     7441 ttacaagaaa aattaagagg aaaaaataga gtaaaagaaa tctgtagata tatatatgaa
     7501 tttttattag atataaatat gccagaaact atagaaagtt taatagtaaa ttttaaggat
     7561 aaaggggaac ttgatgttgc aaatcaatac tcacaagttt gggacatagt agtggacata
     7621 ttagaccaaa tggtggaact tatgggtgat gaaataattt ctttagaaaa atttataaaa
     7681 ttaataacct taggttttga tgaatatgaa ttaggactgg ttcctcctag tatagaccag
     7741 gtacttgtaa gtagtgtgga tagaatgaaa aatccagaca cgaaatattt atacctagtt
     7801 ggaactactg atggtgtgtt tccgctgatt acaaaggata gtggaatatt gagtgataat
     7861 gatagggaaa gtcttggaaa caaaggaatc gaagttgata ttgatagtaa gactaggagt
     7921 tttgaagaac agtttcttgt atataaagct cttacttcga catctaagaa tttaaccata
     7981 acatatccaa tatcagacca tgaaggtaaa acgcttagac catcaataat tatatcaaga
     8041 cttaagaaga tatttccaaa tatagaaaat aaaagttatt tagtggaaga aaataaaagc
     8101 actgataaag atatacttaa aaaaataacg gttaaatccc ctacatttaa tgaacttata
     8161 aacataataa aaaattatga cagtgatggt tacaatacag aagagattaa tagtatttgg
     8221 ttagatatat ataggtacta tttaaaagat gaagtctata gttctataac aaaaaaagtt
     8281 ataaaaggtc ttagttatac taatcaagtt cataagattg aagagaaaaa aataaggtca
     8341 ttgtataaaa gtaattctct tagcatatct aggcttgaaa aatatgctga atgtccattt
     8401 gcttatttta tacaatatgg tctaaaagca aaaaagcgta aagagtattc atttacacca
     8461 ccagatttag gaacttttat tcataatatt ttagataggt tttcaaaaga acttttacaa
     8521 gataatttaa cttggagaga tattgatgaa aaatatatag aattaaagat aggtataata
     8581 gttgatgaaa taattttaaa gataccagga tatatattaa atagttctga gaggtataaa
     8641 taccttgcat atagattaaa gaatatgttg acaactgcta taactataat tagtcagcaa
     8701 ataaaacaag gttcatttga accaatagat tatgaagtta aatttggcaa caatggaaaa
     8761 tatccaccta taaaaatggt gttagaaaat ggacaagaag taagtttaat tggacaaata
     8821 gatagagttg atgaatttga agaaggagaa aataaatata taagaataat agattataag
     8881 tcaggcaata agtccataag tctaacagaa atatattatg gtctacaatt acaattactt
     8941 gtgtatttag atgcaatcct tgaaagtgca aaggatgaag atataaatat aaatccagct
     9001 gcaatactat attgtagaat aaataatcca atagcaaaat ttaatgaaga taaagatgat
     9061 gaagaaatac aagaagcaat attaaaagaa cttagaatga aggggcttgt agtaaaagac
     9121 tctcacatag ttaaagagat ggataaatca ttaatagatg gagaaaggaa aaactcttta
     9181 gtaataccag taggtcttac taaggatgga aatgtaggta aaagtacttc agcaataagc
     9241 tatgaggatt tcaaactgct tagaaaatat gtaaggcatg ctataaagga tttatgtgaa
     9301 gaaatgttaa gtggagaaat acgtatagct ccatataaac ataaagatgg aacatcatgt
     9361 gacttttgtg attactctgc tatatgtcag tttgattcaa ctataaaaga taacaagtat
     9421 aaaaatttaa ataataagag taatgaagag ataataaaaa tgatgaaagg agatgttaat
     9481 tagtgagttc tcctaaatgg acaaaagagc aattagaagt tatagaaagt agagaatgca
     9541 atttattggt tgctgctgct gctggttcag gaaaaacagc cgttctagtt gagcgtataa
     9601 ttcaaatgat aacaagtaga gaaaatccaa ttgatataga taagttatta gttgttacat
     9661 ttactaatgc tgctgcgtca gaaatgagag agaggatagg ggatgctata ggaaaagcct
     9721 tagatgaaaa cccagaaaat aaacatttac aaaaccaact tgttttatta aataaatcta
     9781 gtattacaac aatacactca ttctgtttgg atgttataaa atcaaacttt catagaataa
     9841 acttagaccc taatttcaga ataggagacc aaactgaatg tgctattttg aaacaagaag
     9901 caatagagga agtatttgaa gatttatatg aagaaagaga tgaggggttt ttaaatttag
     9961 ttgaaagtta tgcagaaaga ggaggagata aagaagtaca agatattatc ttgggaatat
    10021 attcatttgc aatggcttct cctgaaccta aaaaatggtt gattgactca gcagaacgat
    10081 ttaatattga tgaaaatttt gatttttcac agtctatttg ggctagagct atattagaca
    10141 ctgtaaaaat agagattaat ggtctttgtc taaatatgga aagggcatta aaagaagtgg
    10201 aatcaataga agaattagaa acttttgcag aaaagttatc tgttgaatat aaaaaaattg
    10261 ctgacatttc acaagcatgt aataaatctt gggatgaggc atataaaaag atggcaagta
    10321 tgtcctttga aaattatgta aaaggagtaa aaagaatttc aaaagatgct ccttcgtata
    10381 taaaagaatc aaaagaaaag gcaaaaacta taagagataa gactaagaag tctttagaaa
    10441 gtatagtaag tgctacattc aataaagata atgattctat tagagaagaa ataaaatatc
    10501 tttataacat agttaaacca atatctagtg ttgtactgag atttgaagag gaatatagca
    10561 ataagaaaag agaaaaagga ataatagatt ttaacgatat agagcatttt gcattaaata
    10621 tacttacaga tgtagatgaa aaaggtaata ttgtaccttc tgatatagca gttggatata
    10681 gaaacaagtt ttatgaaata tttatagatg aatatcagga tagtaatcta gtgcaggaag
    10741 tactgcttaa agctgttgca aatacagaaa ctccaaatag atttatggtt ggtgatgtaa
    10801 aacagagtat atatagattt agacaagcaa agccagaatt atttttgcaa aaatataata
    10861 actataatga taaaaaggga agttcccata gaaaaataat gctttacaaa aattttagaa
    10921 gtagagaaga agttgtagat gctgtaaatt atatatttga aaatataatg aatgagaata
    10981 taggagaaat tgaatataca gaaaaagaaa gactaaattt aggtgcaaat tttaatgtgg
    11041 atacagacga gaaatctatt attggtggag caacggagat acacctaata caaaaagata
    11101 ataaacttga tgatgatatt ataaatgata aagacgatag aattaataac aaagaaaatg
    11161 aaatagagga agaagaaaat ttagataata tacaacttga ggctagaatg gttggaaaca
    11221 ttatcaaaga tttaatgaaa gtcaatgaag atggaaaaat tcaaaaggtc tatgataagg
    11281 gaatagatgg atatagacct gtggaattta gagatatagt tattctttta agagctacat
    11341 ctgcatgggc tccagtgttt gcagatgaat tgatgaatat ggatatacca acgtatgctg
    11401 atgttggagt ggggtatttt gatacgattg aaataaaaac aatattgtca ttacttcaaa
    11461 taatagataa tccaatgcaa gatatacctc ttatatctgt attaaaatca cctatatttg
    11521 gatttacacc agaggatttg attgatatta gagttcagag taaagataaa attttttatg
    11581 aggttttaaa aagtacagca gagtatgatg gatttacaga ttctcaaaat gaaaatgagt
    11641 ctgagtttat accaagtgaa gagtgcataa acaaaagtaa agatttttta attaagctaa
    11701 aggaatttaa agaaaagtca atgtacatga gtacagatga atttatatgg tacttgtaca
    11761 caaggactgg atactatgcc tatgttggag cattgccagg tggaagtcaa agacaagcta
    11821 atttaaaagt attgtttgag agagcaaaac aatttgaaga aaccagtctt aaaggaatat
    11881 tcaactttgt aaattttata gagaaattaa aaaaatctag ttctgatatg ggaagtgcaa
    11941 aaacacttgg tgagaatgca aatgttgtta gaataatgag tattcataaa agtaaaggtc
    12001 tagaatttcc agtagtcata tgctctgcaa tgggtaaaaa tttcaatact caagatttta
    12061 aaaagagtat tttatatcat cataatttag ggtatggacc tcaatttgta gactatgaga
    12121 gaaggatttc ttttccaagt atagccaaag aagcattaaa aagcaaaata aatatagaaa
    12181 atttatcaga agagatgaga gttttatatg ttgcatttac aagagctaaa gaaaaattga
    12241 ttataactgg ttctactaga aatatacaag atagtataaa gagatggtca aatggaattg
    12301 aaagtttaga tacaatatca caatacgaga tattaaaagg taaaaatttt ttagattgga
    12361 taatgccttg tgtattaaga catagggatt tatctaatct actagaagaa gtaggattag
    12421 acgcagtttt taatgttgaa cataattcaa aatggtatgg aaagttgtgg aataagaatg
    12481 atattttagt ggaaaagaag agtgatgaag aaaaagaaag tattgaagaa attcttgaaa
    12541 aaatagatgt agataatcct gacagtgatt attacagtga aatagaagaa aaattaaact
    12601 atatatatcc atatgaattt agtacaagaa aacctgctac tatatctgtt acagaaataa
    12661 aaaagataca aaataattat gaagaagagt taataaatac tatatttgaa caaaaagtaa
    12721 tattaaaaaa gcctctattt attcaaaatg aagaagaaag agaaaaaata agtggaacag
    12781 aaagaggtac aatagtccat ttagttatgg aggttttaga cttaaaaaat gtaagtagtg
    12841 taaatgatat aaaatcacaa ataagaggtt ttgtttcaaa aggtataata acagaaaaac
    12901 aagctagtat agtaaatcca tataaaatat ataaattttt tgcctctaac ataggaaaaa
    12961 gaatgttgaa tgctgaaatt ataaatagag aaaaatcaat ttatgcacaa gtaaatatga
    13021 aagacatata tatttatgaa aagcttataa acaatgacga taaaaaactt tatgataatg
    13081 aaagtgtcat gttaagaggt atagttgatg cttattttga agaagataat caaatagttt
    13141 tggtggatta taaaacagat tttgtaaatg aggaaaatat aaatcaaata atagagaaat
    13201 ataaaaaaca gttggattta tatgcagata ttattgaaac tttaactggt aagtcagtaa
    13261 aagagaaatg tatatattta tttggagttg atgaagctgt ttgttattag tttttatata
    13321 taaattaaac ctaaaagata gaggaagggg aaaaaatgag atttattcat acatcagatt
    13381 ggcatctagg gaagtcccta gaaggccatt ctagaattga ggaacaagca aagttttgtg
    13441 aggaatttat aaaaatagta gaatcaaatg aaatagatat ggttataatt gctggagatg
    13501 tatatgatac ttctaatcca ccagcacaag cagaaaaact attttatcag actgtatcaa
    13561 gacttgccaa taatggtcag aggtgtgtat taataatttc aggaaatcat gataatccag
    13621 agagactctc tgcaataaca cctctagcac atgaacaggg gatacttata tatggttatc
    13681 cattaagtgc tacgatagaa gctaagtata aagggtttga aataacatat gcaactcagg
    13741 gatgtacaaa actgaatata aatggagaaa atattgtggt tgctacatta ccttatccaa
    13801 gtgaaaaaag attaaatgaa gttttttcaa gtgaagatga gtttgagaaa caaaaaaatt
    13861 attctgaaaa ggtaggagat atatttagaa gtttagaaga aaattttaga agtgatacta
    13921 taaatatagc cgtatcacat atatttgtaa taggtggaga gagtactgaa tcagaaagac
    13981 ctatacagtt aggtggaagt tttttagttg aaagaaaaga tttgccagaa aaggctcaat
    14041 atactgcact aggtcattta cataaacaac aaaaagcttc cgaacgttta aatgcatact
    14101 attctggttc tccacttcaa tatagcaagg atgaaagagc gtataccaaa ggggcataca
    14161 tagtggatat aaaagctggt gaaaaaccaa taattgaaga tgtatatttt aataattaca
    14221 aacctataga agtatttaaa tgtaatggaa tagaagaagc actagatata tgtgagcaaa
    14281 atcaagatag agagatttgg tcatattttg aaataaatac tgatgagata atatcacaga
    14341 atgaaattaa aaaaatgaag gaactattaa aagacataat agaaataaaa ccaataataa
    14401 catcttgtta tgaacaagaa agtgtagata taaaagaaaa gtctatggca gagttgttta
    14461 gagagttcta ttcttttagt aaaggtgtag agccaaaagg agaacttatg gatttatttc
    14521 tagatataat aagtgaagaa ggtgaatcag cagatgagac caattagatt agaacttaca
    14581 gggcttaata gctatattga taaacaagtt atagactttg aaaaattaat tgaaagaggt
    14641 ttgtttggga tatttggaac tactggaagt ggaaaatcta caatcttaga tgcaataact
    14701 attgctatgt atggaaatat atccagaaat acaaaagaat atataaacag tgtgtgcgat
    14761 aaggcaatta tatcatacga gtttgagata ggaagcaaaa acactagaag aagatacatt
    14821 gtagatagaa ccattgctag aagcaaaaca ggaacaaaga cctctaatgc tagattaata
    14881 gaagtattga atgacaatac ccaaaatgtt ttagctgata aagttgtgga agtaaatgaa
    14941 aaagtagcac aggttgtagg attaacggca aatgatttta ctaggtcagt agttttacca
    15001 caagacaaat ttaatgagtt tctaagatta tcacgtgcag atagaagaga catgttagag
    15061 agaattttta atcttgagaa atatggaaga agtcttggag aaaaagtgaa aaagagaaaa
    15121 aatatacagt tacaaaattt aaaagatttg aaatcaaaat taagtcaata tgatggaatt
    15181 acagaggaag tatacaacaa aataaaccaa gaattaatag agttaaaaaa tttggaaaaa
    15241 gataagaata atgctttaga cctagctcaa aaatcttatg aagaaagtaa aacagtatat
    15301 gaagaacaat taaagttaga aaaaaatgaa cttagaaaaa atgagcttga tttaaaaaat
    15361 agtgagataa aagaaaagat tgctcttgta gaaaatgata gtaacgctaa aaaggtagac
    15421 ccatacataa gttcagttca aaatctagaa aaaaagctag atgaagatag ttttgcagta
    15481 agtaatttag aaaagaaatt agcaatatta aatcaggagc ttgaagttac aaaaaataga
    15541 tatgaaaaaa taaacaaaat taagaatgaa gaagtcccta aattgagtga agaaaaaata
    15601 agattgcaac aagcaataaa attagaagaa gagttagttt tattagatag agagttaaaa
    15661 gagctaaaag aaagtggaat taatttaaat aagactaaag ttgaattaga aaaagtaaag
    15721 caggtgtcag agtccagaaa agatgcagtt actaaatcta taaaagaagt tgaaggaaag
    15781 attgataaag taaatataag tgcagaatta aaacaaaaaa tatttctagc atatgaatac
    15841 gagaaagatt acaacaacgt attggaagaa aaaaatcaaa agctaaataa actagaagaa
    15901 atactcaaag agacagaaaa tataaattta aaagttagat atattgataa agataagaat
    15961 gatgtaaatc gtaatttaga aaatttatca cttcacttgg atgttcttct taaaaaatgt
    16021 cctggtaaga gtgctgattt acttttaaaa tctgaatatg ttactgaatt aagaaataaa
    16081 gcaaataata ctaaagaaaa tgaaattaag aaaagcagta tacaagatga attaaagata
    16141 atacttgaaa gtaagtttaa tactgaaagg gaagtaaatt tactaaatga aaaacttgaa
    16201 aataatagaa agaacagaga tgacttagaa aaagaattgg aagaactaaa gtatttaaat
    16261 ttagcctctg aacttagacg tgaattaaaa gaaaatatgc cttgtccagt ttgtgggtca
    16321 aaacatcatg aaaatcatga tataactaag tatgatgaaa atatatcttt tgtcaaagaa
    16381 aaactggaga aattagaaaa agaaaaaata agtataagaa acaatataga ggaattaaat
    16441 gctaaagtaa gtgggtattt atctatagaa aaaatgaaga ctaaagaact tgaagatgta
    16501 aaaggaaaac taggggggat tccatctagt caattattaa aaaaacttga tgaagaacaa
    16561 agaaaacttg cattgttaaa atctaatatt caggagtggg agaaagaaaa agagagtact
    16621 gaaaataaag taacattagc taaagaagaa aaaaataaaa ttgaaaaaga agaattaaaa
    16681 ataagagaga gtctgaataa ttataagaaa ttaacaagag atttaaacat agaaatagaa
    16741 ggtttagaaa ataaatgtaa aaaaattaaa caggaatacc taggattaaa gacgattact
    16801 aaagtctcta atctattgtc aaaagtaaaa gaaataagag agaatgaaaa agaattagaa
    16861 ttactaagta ctgggcattc aaatttatta aaaaatagag actcactaga tattaaaata
    16921 agagaaaatg agagtaaact acatgaaata gagatagaat taataaaggc tagagaatta
    16981 tatgtagaaa agaaattgag tagggataat aagtataaag aagttattag cataacgaaa
    17041 ggtgatttag ctaaaaatct tttacataat gttgaagaaa atatatgtaa gatattagaa
    17101 caagaagaat caagtaagaa aaaattggaa gagcaaagat tagaatatga aaaaaacatt
    17161 gctgaaaagc acaatataga tggtagactt aaaactgcta aggaacaata taaagaccaa
    17221 aaatcaacac taaataaact attagcagaa aataaatttg aaagtatata tgcagtaaaa
    17281 agagctttat tagatactga tgcagtaaaa aaattgattg aagaaatact agagcatgaa
    17341 gaagaacaaa agttattatc atttaaaata aaatcatcaa aagaaaaatt aaatggaaga
    17401 agcataaaga aggagtactt tgaacagtta aaagatgaaa tttataattt gaaagtagaa
    17461 ataggaaata tatccaaaga tattggagct aatcaaaatc agttaataac attaaaagac
    17521 tctttagata agataaatga ttttaataaa caacttaagg tagttgaaca caatgtggac
    17581 ttattggaag aattagataa atgtgtacaa ggaaataaat ttgtagaata tgtttctaca
    17641 aaccaattaa aatatatagc attggaagca tcaaaacgat tggatggaat aacaaaagga
    17701 agatatgcac ttgaaattga ctcaacacta aactttgtaa tgagagataa ctttaatgga
    17761 ggagaaagaa gaagcattga tactctatct ggtggagaga cattcttgac atctctatca
    17821 ttagcacttg ctctttcatc tcaaatacaa ttaaagggaa gtgcaccatt agaatttttc
    17881 tttttagatg aaggttttgg ttcattagat agtgaacttt tagaaattgt tatagaatcc
    17941 ttggaaagat tgcatagtaa taatctaagt gtgggtataa taagtcatgt cgaggaactt
    18001 aaaaatagag ttcctgttaa gctattagtt tcatctagtg aggctggaat aggttcaaaa
    18061 gtaaaaattg aatatagtta gaaaatatat gttaattgaa tatattatct aatatttcca
    18121 aataatatgt aattgtgata taataaaaac taatattaaa gttatttata attaatatta
    18181 atataaaaac atgtaagtag gagtgtggta agtgttggaa tcgcccaata atttgattca
    18241 gattattttt ttaatagtat tacttatagg gtctgcattt ttctcagcat ctgaaacggc
    18301 cttaatgtcg ttgagtaaaa tcagaatcag atatatgcaa gatgaaggag ttaaaggagc
    18361 taagttagta agttcattaa tagaaaatcc aaataagctt ttaagttcta tattggttgg
    18421 aaataacgtc gtaaatatag cagcaacatc catatcaaca tctttgttta taggattaat
    18481 gggagaaaaa ggagtggcac ttgctacagc tgttatgaca gtattagtat taatatttgg
    18541 tgagattact ccaaaaacaa tagcagcaaa taattcagaa aaggtttctc tattagtatc
    18601 taaacctata aaagcaatta tatttatatt aagaccaata gtatggatat ttaatataat
    18661 tacaaatatt atatttaaat tatttggtat aacaaataaa ggagctaaat cattcataac
    18721 tgaagaagag ctaaaaacta tggtaaatgt aagtcatgaa gaaggcgtcc ttgaaatgga
    18781 agagagagaa atcataaata atgtttttga atttggagat atgcaagcta aaaatgcaat
    18841 ggtacaaaga atagatatgg tagctataga tatggaagat agttatgatg agataataca
    18901 ggtatttaaa actgaaaaat taagtagaat gcctgtgtat gaagaaacta tagatgatat
    18961 cgtaggaata cttaatataa aagatattat atttttatct gatgaagaaa tagagtcatt
    19021 tgatattaaa aattatatga gagaaccttt tttcacgtat gagttcaaaa agataacaca
    19081 acttcttgaa gagatgaaac ttgaaaaaag tcagatggct atagttgtag atgagtatgg
    19141 tggaacatct ggacttttaa ctatagaaga tttagtagaa gtcattgttg gagatataga
    19201 ggatgaatat gatgaagaag aggatgaaat acaagttata aaagaagatg agtatattgt
    19261 agatggaagt actaaaattg gtgatgttaa tgaacttatt ggtgtaaact tggaatctga
    19321 ggagtttgat tccataggag gttttataat tggacaccta agcagattgc cagaagaaaa
    19381 tgaagtaatt gaagttgata atataagatt ttgtatagaa agtatagaaa aaaatagaat
    19441 taaaaaaata agaatatata cataatg
//
LOCUS       DAEOSP010000057        19159 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.057, whole genome shotgun sequence.
ACCESSION   DAEOSP010000057 DAEOSP010000000
VERSION     DAEOSP010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19159
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.057"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            102..1316
                     /locus_tag="KRH54_001658"
     CDS             102..1316
                     /locus_tag="KRH54_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBG8579324.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            1493..3406
                     /locus_tag="KRH54_001659"
     CDS             1493..3406
                     /locus_tag="KRH54_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8579325.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKLSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            3677..4651
                     /locus_tag="KRH54_001660"
     CDS             3677..4651
                     /locus_tag="KRH54_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG8579326.1"
                     /translation="MHILKSVKKIPGGLMVCPLLFGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            4672..4947
                     /locus_tag="KRH54_001661"
     CDS             4672..4947
                     /locus_tag="KRH54_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG8579327.1"
                     /translation="MNAIIHANEKDNLVTCVRPLIKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            4960..6123
                     /locus_tag="KRH54_001662"
     CDS             4960..6123
                     /locus_tag="KRH54_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG8579328.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            6460..8739
                     /locus_tag="KRH54_001663"
     CDS             6460..8739
                     /locus_tag="KRH54_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG8579329.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSTGDTYFEINDSEKEVNKYLSKKLQLFEFDWLG
                     IIDSNGNFISALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYKGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSIYYTLNDGISKAMHYLPLQEENLYLLSVVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDMEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(8834..10174)
                     /locus_tag="KRH54_001664"
     CDS             complement(8834..10174)
                     /locus_tag="KRH54_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8579330.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEEILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(10186..10968)
                     /locus_tag="KRH54_001665"
     CDS             complement(10186..10968)
                     /locus_tag="KRH54_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8579331.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(10983..12005)
                     /locus_tag="KRH54_001666"
     CDS             complement(10983..12005)
                     /locus_tag="KRH54_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG8579332.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(12011..13024)
                     /locus_tag="KRH54_001667"
     CDS             complement(12011..13024)
                     /locus_tag="KRH54_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG8579333.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFIAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(13152..14090)
                     /locus_tag="KRH54_001668"
     CDS             complement(13152..14090)
                     /locus_tag="KRH54_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG8579334.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATESDKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            14360..14824
                     /locus_tag="KRH54_001669"
     CDS             14360..14824
                     /locus_tag="KRH54_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579335.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(14936..15253)
                     /locus_tag="KRH54_001670"
     CDS             complement(14936..15253)
                     /locus_tag="KRH54_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG8579336.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(15266..15997)
                     /gene="chbG"
                     /locus_tag="KRH54_001671"
     CDS             complement(15266..15997)
                     /gene="chbG"
                     /locus_tag="KRH54_001671"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBG8579337.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(16023..17333)
                     /locus_tag="KRH54_001672"
     CDS             complement(16023..17333)
                     /locus_tag="KRH54_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG8579338.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(17430..18743)
                     /locus_tag="KRH54_001673"
     CDS             complement(17430..18743)
                     /locus_tag="KRH54_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8579339.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(18765..19076)
                     /locus_tag="KRH54_001674"
     CDS             complement(18765..19076)
                     /locus_tag="KRH54_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8579340.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
BASE COUNT         6863 a         3055 c         2628 g         6613 t
ORIGIN      
        1 ttatctatac atacccatat aactagataa ttaaaatgta taatattatt tgctatagaa
       61 aataatacac tcatacataa taaattttag gagtgaagta tatgaatcaa gaatatgtaa
      121 aaggaaatcc tatttttact aatattaata aagttccaag acaatttcca taccttactg
      181 atgatattga tactgatgtt ataattgttg gtggtggcgt aactggatgt atctgtgctt
      241 actatctagc taaaaacaat ataaagtctg tcattcttga aaaaggaagg attgcacatg
      301 gaagcactag cgtgacaact tctctattac agtatgaatt agatgacaat ttgatagatt
      361 taacagaggt tatgactcta aaagatgctc taaaagcata taacctttgt acttcagcac
      421 ttgaagaatt agatacattt atagagctat atggaaataa gtgtgattat gccaaaagag
      481 atactctact ttatactgcc aacaaacttg aagttaaggc tatcaaagaa gaatataact
      541 taagaaaaga aaatggattt gatgttgaat atattgatga atccacaaac ccttttagct
      601 ttgatttaaa atcaggattg atagcaaaga atggaggacg agaattagac ccatataaat
      661 atagtcacca tttgattgat gtaagtttaa aaaatggtct tcaagtatat gaaaatacag
      721 aagtaaaaaa agtagattta tcaaatgata aggttactgc tgaagtatcg tatggataca
      781 aagttcatgg taaaaaatta attgtagcta cagggtacaa tacaagttta tttacaaaaa
      841 gaaattttgc aactaagtca aatactttta atatcgcaac gaaaccactt aaaaacatag
      901 cttcttggaa aaacaacatt ttaattagag ataactgtga cccatataat tatctaagaa
      961 ctactaaaga caatcgatta attataggtg gtgaagatgt aagttttgat gatatagaaa
     1021 atgaaacttt agccaatgaa aagtacgata ttttagaaca aagattgaaa agtatgttta
     1081 aggatataaa agacattgaa gttgaatata aatattgtgg ttgttttgct tcaactttag
     1141 ataatttagg ttttattggt ccagataata aaaataacaa tctatggtat tgcttaggtt
     1201 atggagcaaa tggtatactc tttgcaatat taggaggtat aatgttaagt gaattatatc
     1261 ttggcaaaca aaataaaaat atgaaattat ttaaagtaga tagatttgat aaatagatat
     1321 tatatattaa aagactgtaa aaagatgaaa ctttataatt ctaaaagtta actttgatac
     1381 agtcttttat aatgttcctt ttaaaaatac tttatttata attaaatata aagtaaaatg
     1441 ttaaaatata actaataaaa tttagtcata agataatatt taagtttatt tgatgaaata
     1501 tggaggaatt attatgaagg aaatactcgt tattgctccc actaaaggaa cctatgaaaa
     1561 atcaattcac atagttaaaa aaaacaaata tacaaatatt gatgttgtat ttggcaatct
     1621 aaaagaaggt atacctcttg ctgaaaaaag tattaatcat ggtactagaa ttatcataag
     1681 ccgaggtgga acttacaata tgttaaaagc tacttacaat atacctatag ttgaaattaa
     1741 ggtagatgct tatgatatta taaaaagcta caaagaggta aaaaattcca acgaaccatt
     1801 tggaataatt ggatttaata atgtcatcta cggtttcgat attatagaag aaatattgaa
     1861 taagaaaata actatgattg aaattgaaaa agaagaagaa atttatgatg ctatagaaaa
     1921 gtacagaaag aaaggtataa acacatatat aggagatacc actgttgcac atattgttaa
     1981 gagacttaat tgtaagggaa tattaatcga atctagagaa gaaaacatac ttagagccat
     2041 tcaacaagca gagcaaatac tcgaagcaac caaggatgaa caaaaaagaa gattacagat
     2101 agaagctatg acagattttg tacatgatgg aataataaca gttgataaaa attttaaaat
     2161 aacattattt aataagagtg ctgaaaaaat atttggtata aaaaaagaaa atgcacttta
     2221 tcataatgtt gtagatgtta tcccaaacac agttcttcca caagtagttg agactatgga
     2281 acctcaaatt ggtgaaatac aaaatatagg gagtacaaaa attattacaa atagagttcc
     2341 aatcattgtt gatggtcaga ttcaaggagc tgtttcaaca tttcaagata gcaaggagat
     2401 aagttctttt gaacatacaa taagaaggag cttattaagc aaaggtctat ctgctaaata
     2461 cacttttgat gatataattc atgctagtga aaagacaaaa gattgtatag aacttgcaaa
     2521 aaaatatgct tgttatgata ctcctatgat tattactgga gaatctggag ttggtaaaga
     2581 actattttgt caaagtatac ataactatag taagcgtaaa aatgcacctt ttgttgctgt
     2641 aaattgtgct gccatacctc catctctaat agagagtgag ttttttggtt atgaggaagg
     2701 ttcatttaca ggtgctagaa aaaaaggtaa agcaggtgta ttcgaactag cacatgaagg
     2761 cacaatcttc ctagatgaaa taagtgaaat ccctcttgaa cttcaaggtc gattattgag
     2821 ggtattgcag gaaaaacaag ttatgagaat tggtggtgat aaagttattc ctatagatgt
     2881 aaaaattatc tgtgcttcaa ataaagactt aaaaaatatg atgcgcaatg gtttatttag
     2941 acaagacctt tattttagaa taaatatact aacattatat cttccttcat taaaagaaag
     3001 aaaagaagat attataaaat tatcagaatt ttttatacat aaatattcaa gcaaatacaa
     3061 taaaacgcct ttaattatca ctccaaatat taaaaaggca cttcttgaac gagaatatga
     3121 aggtaatata agggaattgg aaggtatgat ggaaagggct gttattgtgg aatcatttga
     3181 ctcgatttta ttagaaaagc ttagcaatga atatacaaat aatatagttg ataaagataa
     3241 tagtgatttt gaatgcagta atttagattt aaaaacttta gaaaaaaaat atatctataa
     3301 aatttataac gaaaataata gaaatactac aaaaacctgt gaaattctaa agataactcg
     3361 ctccactcta tggcgtaagt taaaagaaat agaagatgat gtttaaaaat aatacatatc
     3421 tatatatatc aaaacgtttt atgtttaatt ttaatacata agacgtttat ttgttttcaa
     3481 taaaaaatac aattatatga taaaaagaat ctttgtaagt gaaattctcg tcaatcatac
     3541 ttttaattat aagtaatcta cagcatttca atcgattatt aaaaataatc ataataacta
     3601 aagatacaaa ctctatatat tggcatagta attgctttta taatacctat ataaaaatga
     3661 aagggagtga tactagatgc acatcttaaa atcagtgaaa aagataccag gaggacttat
     3721 ggtctgtccc ttgttatttg gagcactatt taatacagta tgtccacaag ctcttgaaat
     3781 tggaggattc acaacatcct tatttaaaac tggtgcaatg agtatacttg ctctattttg
     3841 tctatgcaat ggagctcaaa tcaatatcag acaagctggt aaaccattga ctaaaggtat
     3901 agtacttaca gcaacaaaat ttataattgg tgcagtatta ggtattattg ttagtaaatt
     3961 tattggacct aaaggtataa ttggtattac acctcttgcc atagttgcta ccatgactaa
     4021 ttcaaatgga ggtctatttg ctgctcttgc aggtgaatat ggagactcca cagatgttgg
     4081 agcaatatct atcttatctc taaatgatgg tccttttttt acaatgcttg cttttggagt
     4141 aactggactt gcaaatgtac ctatagtagc actatttgca gcacttatac caatactact
     4201 tggatttata cttggtaacc ttgatgaaga tatacgagaa tttcttgcac ctggtacaac
     4261 attattaatt cctttcttcg cttttccttt aggagcagca ttaaacttta atcaaattat
     4321 aactgctgga ctacctggag ttgtacttgg tttaggagtt actataataa caggtatggg
     4381 tgggtacttt gtaatgaaac ttatacgtgc agaacatcca gcagtaggtg cagctacagg
     4441 tacaacagcc ggaaatgcag ctggtactcc tgaagcttta gcagctatag accctactct
     4501 agctacaata gcagctgttt caacagtaca agttgctgct gctattattg ttacagcaat
     4561 atgttgtcca atgttagttt cctatctaga taaaagagaa aaaaagaaaa aatctatttt
     4621 taaaacaaat aaaaatattg aagtaaatta atacttaagg aggtaaaatt aatgaatgca
     4681 attattcatg caaatgaaaa agataatcta gttacatgtg tcagaccttt aattaaaggt
     4741 gaaaaagttt caattgatgg aaagtcttac atagtaaatg ataatatacc tgtctttcat
     4801 aagatggcta cagaagatgt aaaaaaaggt gatgttgtat acaaatacgg tgaaattata
     4861 ggaattgcaa cagttgacat aaaaactggt gactatgttc atgtacataa cattgaaagt
     4921 acacgtggtc gtggagataa aaaataggag gaagttaaaa tgaaattaat gggttattta
     4981 agagaaaatg gtcagtttgg tattcgtaat catgttttaa ttattccaac atctgtatgt
     5041 tcaagtgaga cagctacaag aatagcatca cttgtgcctg gagcaatagc aatccctcat
     5101 caacatggat gttgtcaaat tggttctgat atagaactta cagcaaagac attaatagga
     5161 tttggaaaaa atcctaatgt tgccgcagtt ttagtagttg gtcttggatg tgatggtatt
     5221 caagcaaaag aattagcatc tgaaatagct acaacaggaa aaaaggtaga ttacgttgtt
     5281 atacaagaat gtggtggaac attaaaaaca gtttctaagg gagctgaaat agtaagtaaa
     5341 atggctcgtg aagtatcaaa agaagtaaga gttgaatttg gtatgagtga aattacatta
     5401 gcacttgaat gtggtggttc tgacccaaca agtggaatag cttcaaaccc ttcaataggc
     5461 gttgcatcaa accttcttgt agatgaaggt ggaagtagca tactaagtga aactacagaa
     5521 gtaattggtg cagaacattt gttggctacc cgttttgaag atgaagaaat gaaagataag
     5581 tttcttaaat ttgtaagtga tgtagaaaaa agagcaattg ctatgggaga agatttacgt
     5641 agtggtcaac ctactccagg caataaagct ggtggacttt caacaattga agaaaagtca
     5701 cttgggtgta tgtataaggc aggcaataag ccattcaaag gtgctcttga atatgctgat
     5761 attgtacctc ctgataaaaa aggattatat tttatggata ctccaggtca agatatagat
     5821 tctatcactg gtatggtagc aggtggtgca caaattgtaa tcttttcaac tggtagaggt
     5881 actccaacag gaagccctat ttcaccagtt attaaaatca ctgggaatag tgatacatac
     5941 aacaaaatgc cagacaatat cgatataaat gcaggtagaa ttataacaga tggtgctaaa
     6001 atagcagata taggtcaaga aatatttaat gaaataatag aagtctgtaa tggtaaacat
     6061 accaaagctg aaagtcttgg tcatagagaa tttggcattt acagaatttc atctacattt
     6121 tagattgtaa caaaatatct tttataaata taatacttat gtagatacat aagctaaaag
     6181 ttctttctaa tcctataaaa attttctata ctgaaaagga actgtatcaa aattattttg
     6241 aaaatagttc cttttttcac ctcttttact caaaatatta taaaataaga tttaaacaat
     6301 aaacgtatct tgattaatct ctaagaagac ataaataaag ctaaatgtaa agtgagataa
     6361 ataaaattta atatctttgt atgaaaagat atttggtata atatgtctga gagaattctt
     6421 atttgtaaac aaataaagga tggaggatga aacaaaagaa tgaactttga aaacacaaaa
     6481 aataaaaaaa taatagctac tttactattg acactccttt taatcgtgtc attaacatgt
     6541 gtcactttta ttatgaatct aaataaagta acacataatg aaacagaacg atatcttgta
     6601 gaagtttcac aaagtatttc cactacaatt aatgtgaaat gtaaaagtat atttaggaca
     6661 ctatattcta cgggagatac ttattttgag attaatgata gtgaaaaaga agtcaataaa
     6721 tacttatcaa aaaaattaca attatttgaa tttgattggt taggtattat agattcaaat
     6781 ggaaatttta taagtgccct taaagataca ggtaatgtta aaggacaaga cttttataga
     6841 aaagcccttc aaggtaaaga aacaattact cctgttaaat cacaatctgg tacatatgga
     6901 attttatatt ctgtaccact atacaaaggt gataaaattg ttggagctgt tgctgcttgg
     6961 aatactattg atactatgag aaatttatta agtgtagaaa gctttcatgg tgaaggattt
     7021 tcccaaatta tcgaccaaga tggaaatttt attgtaagtt cagcaaacaa aaatgctcca
     7081 aaaaatgtgg tgaatttttt tgacatgatt aaacaccaag gaaaacaggt tgataaaaat
     7141 aaacttgaag aaatgaaaat aaacttaaaa aagaacaaaa ctggttctat atattacaca
     7201 ttaaatgatg gtatttctaa ggcaatgcac tatcttccat tacaagaaga aaatttatac
     7261 ctcctttcag ttgttccaac aaaaacagca agtgcacagt ttgatttact tctaaaaaaa
     7321 gcaatcttca tcaatatttt aattgttata ctatttttat tgcttattat attgattatg
     7381 tttataaatc aaaaaaacag ggcacaactt ataaaaatcg cctatgtaga ctctgtaact
     7441 aaaggttaca gtaaagcacg ttttgatatg gaagctaaaa aaattataca gtcatcttca
     7501 tcagaaagct attcattagt atctataaat attcagaaat tcaaacttat taatgattct
     7561 tttggtagtg aagcaggaga taaaacatta aagtacatac atgatgtcat tagtaattat
     7621 ctagaaccag gagaattact ttgtcgagtt tctaatgacc agtttaatgt acttattaaa
     7681 actattcctc aaaaagaaat tcttaagtac atagaaaata tcaccaatga tattaatagt
     7741 ttcaataaaa atgaagaatg taaatacttt atagctctat ctataggtgt atataaaatt
     7801 gatgacccaa atctaccttt agtttcaatt caagaccgtg caaatgttgc acgaaaaaat
     7861 aataaaggaa acattggaaa tcatccactt tatacatgtg ttttttatag tgatttagag
     7921 cgtttaaaaa tgattaaaga aaaagaaatg gaaaatagaa tggaggaagc attaaataat
     7981 aacgaattta tagtatatct tcagcctaaa ataagcctca aagataatac tattgtaggt
     8041 gcagaagcat tagtaagatg gcaagaccca aataaaggtc ttataccacc agacgagttt
     8101 attcctttct ttgaaaaaaa tggttttatt acaaaactcg accactatgt ttttgaaaaa
     8161 gtatgcagta tgataagaaa atggatagat aatggagaaa ctccaatacc tgtttcagtt
     8221 aatttttcac gtgctaattt aaataatata gattttttag aaaaatacag aatgcttcgt
     8281 aataaataca acattcctgc taagctctta gaaattgaat taactgaaac acttgtattt
     8341 gaaaatctcc aaaaattaat taatataata gaccaaatcc atgaagaagg ttttcaatgt
     8401 tcactagatg actttggaag tggatactct tcactcaata tgttaaaaga aataaaagtt
     8461 gacacattaa aacttgaccg tgcttttttt agctcaccta atgcagataa tattgctgaa
     8521 aatcatgtaa tagaatctat agttaatttg gcaaaaaaat taaatatgaa tagtatatca
     8581 gaaggtgttg aaacaatctt gcaaatggat tatcttaaaa aaattaattg tgatatgata
     8641 caaggatatg ttttttcaaa acctctacct caagaaatat ttgagaaaat gacttttgga
     8701 aaaaaactag aagaaaccag tatggatagt tccgtttaaa taaaataaaa aacttgtctc
     8761 tataaaataa ttttaatcat tttataaaga caagtctttt tattttccaa tttaatttta
     8821 ttttctaaat ttcttacact cggcttgttt taaattccct ttttaagaat aataatacga
     8881 ttatcatagc aataaaatct gcaactggct gtgccaacca aacaccatct aaattaaatt
     8941 gaattggaag tacaatgatt aatggtataa ataaaataaa ttgtcttaaa agagttaaaa
     9001 acattgaatg tttaacttta cttatacact gaaaatacac aggtcctaca atagaaatcc
     9061 ccattatagg caatgtaatt agatttaggc ttatacctct tactgctata tccatcaaat
     9121 ctgaatcttt attaaatata cctacaaata cttctgggaa aacttgtact actaaaaatc
     9181 caaatgacaa tatgacaact gacactatga tagataatat taaggttttc tttgcacgct
     9241 catattgttt tgcaccataa ttgtatgata ttatagtttg cataccttga ttcaatccaa
     9301 acacaggcat cataaacatc aataaaatag atgtaagtgc tgtcatagct ccaatagcta
     9361 aatctccacc ataaaccttt agagaattat ttgtaaacat gtgagttata cttatagcta
     9421 cttccatagc aaaaggtgtc attcctattg caaatacctt ctttactata tctttattca
     9481 attttaagtt acattttttt agttttaaat tggacttccc tcttataaaa taatgtatta
     9541 cccaaaaaaa tactactatt tgacagagta ctgttgctat agctgctcct tttataccta
     9601 aattaaatac aaatataaat atagggtcca atatcaggtt taatacacat cctacaatca
     9661 ttgtccttgc tgctaatttt ggattacctt ctgccctaat tgcattatta agagaaaatg
     9721 caactaaatt aaatatactt ccaaccaata tgacactcat atagtctttt gcataaaata
     9781 ttgaatcttt acttgcaccc aaaataaaaa gtatctcttc taagaaaact aatcctaata
     9841 tagttataat taaagagata attatagata atgtcaaggc attacctaat atcttctctg
     9901 cttcttctct atttctttcc cctaacttta tagacaatct tgtgctagca cctactgata
     9961 ccaacattcc aaaagctatt attagagtaa atactggcat agttacacca agacctgcaa
    10021 tagctataga gccaatacct tccatagaac caataaaagc tctatctact acattataaa
    10081 gtgaagttac catcattgct attattgcag gtatcgaata cctaaataat aaaatccata
    10141 ttttttcatg tcttaatgct tcttgattct ccatgttgtc accatttaca ccatgtcata
    10201 agttatacaa attggtttat ttgttcttgg gtctgttgct atttctgcat caatattaaa
    10261 taattctttt agattatcct tcgtcataac atcatatgca tcaccttcac aaactatttt
    10321 acctttctta atacctatca tgtggtcagc aaatcttgct gcattattaa gttcatgtat
    10381 taccatgaca atagttcttc cttgtttttt atttagttca tctaataatt ttagtatctc
    10441 caattgatgt gccaaatcta aatatgttgt aggctcatcc agtaataata tatcagtttc
    10501 ttgtgccaaa gccatagcta tccatgccct ttgtctttgt cctcctgata aatcttccat
    10561 atttctatcc ttaaattctg caattccagt aacttctaaa ctccaattta caatctctct
    10621 gtcttcttta ctagaattac caaatccact tttatgagga aatcttccat aagaaattaa
    10681 ttctgaaacg gtaagaccac taggagcttg agggctttgt ggaagtacag ccatatcttt
    10741 tgctatttct ttagacttct ttttgtagat gtcctctcca tttatatata ttttcccact
    10801 attagggcta ataattctac ctatagtttt aagtattgtc gattttccac aaccattagc
    10861 ccctatgatg gtagttattt ttccttttgg tatatttaaa tttaaatttt ttataatatc
    10921 tgtgtttcca tatgatacat ttagattctt agtatttata cagttcataa caacaccctt
    10981 tcctaatcat ttaccaacat taaataaata aagtacggca ctcctattat agaaactact
    11041 atgcctactg gtatttctat aggtgctaga agatttcttg atatagtatc tgctattaaa
    11101 agtaataatg ttcctatcaa tgctgatact ggtattaatc ttttatgctt tggtcctatt
    11161 atttttctac ctatatgtgg agctattaaa cctaaaaatg aaatactacc tgctactgct
    11221 gtcgatacac cagataaaat tactgcatat attataagtc ttcttctttg tttttctaca
    11281 tctacaccaa gacttataga tacttcatca cccaaattta aaacatctat atatttagat
    11341 ttataaatag ttatacccat aaaaatcact ataaatggaa gcacagctat tacatatttc
    11401 caactggttc cccaaatgct tcctgatgtc catgccataa ccctattaaa ttcttgtgtt
    11461 gtaaatttca attggaatat cgtcaaaatt gaagtaaaag ctatgtttac acctatacca
    11521 ataagtagta atcttgacga atttatacca cctttccatg caagagaata tataagaaat
    11581 gctccaaata aagctcctat aagtgcaaca ataggcattg taaatacagt caagttactc
    11641 ataccttcat atacattacc attcataagg tatatataaa ctactacaaa caatgcagca
    11701 cctgaattta ttcccaaaat tccagaatca gctaaatcat tctttgtaac tccttgaagt
    11761 attacacctg ctgttgataa tgcagaacca accaaaagtg ctattactat tcttggtaat
    11821 cttaatttaa atattgcaat ttcatgatta cgctgaccct gccctaaaaa agtctttata
    11881 acatccattg gttctataga aaatgagccc atatttaaac tcaataaaaa agtgataaaa
    11941 ataagcacca ttaaaattga tataacaagt aatgttttag aatttatttt tataatattt
    12001 ttcatagtac ctatttctcc ccctttctaa caaggtatat aaatattggc actcctataa
    12061 gtgctgttat agaacctact ggtgtctcat atggaggatt tatcatccta cttaatatat
    12121 ctgtatatac aagcaataca gctcctaata ctaaagagct cggtattata tacttataat
    12181 ctgaacctac tattccttta gctatttgag gaactataag acctataaaa ataatattac
    12241 cagacactga cacagatgct ccagtcatta atattacaag tactatacat ataaatctaa
    12301 caaagttagt tttttgtcct aatcctattg ccacttcttc tccaagactt aatatagtta
    12361 ttcttggagc cataataata gctaaaataa atccaatacc tccagctata aatagcaatt
    12421 ttataccttc ccacttcact cctacaagcc ctcctgatat ccagaaactc aactgttgag
    12481 ataggttaaa atacatagat attgctgatg ccaaagaaat taacaatgta ccaagagcta
    12541 ctcctgcaag tgctagtttt acattattaa ctcttcctct tgatttagaa cttataaaat
    12601 atataaataa tccacttata cttgccccaa taaatgcaaa catcattaca ctaaaacttc
    12661 catgtataag ttgaggaagt tttttttgaa gtaccagtga tatcgaaatt gcaaatgtag
    12721 ccccttgtgt aattcctata acagatggtt cagctatagg atttctcgtt attccttgca
    12781 ttattgcccc ggaaactgct aaaaaacctc caacaagtgc agctgctatt gcccttggaa
    12841 gtctaacatc ctttactatt ctcatattta ttccatcttc catttttatc aagctatcta
    12901 taacagttga tagattcata tcttttgccc caatagttat ggataataca attccaaata
    12961 caagtacaac tatacttact aatatcaaca aataagttat atttttttta cttaacttag
    13021 tcaattatcc tacctccttt tcccttttaa tcgtcagtat aatatcatct attaagtatt
    13081 ttaattttaa gctttattaa aaataggtgt gaaaaatgaa tatcattatc tcacacctac
    13141 taagttaaga attactttgt taattcattt ttaactgatt ctaataataa ctcttttcca
    13201 attggattgt atgattgact gaaaaatgga gcagcgtcaa gtattgttac attgccatct
    13261 tttactgctc ttatttgagc ccagatagca ctattttcta aatctttttt atctgattct
    13321 gtagcaatta caactatatg gtctgcatct atatctgtta aaccttccat acttaccatt
    13381 ggtagtgtta ttccatcctg ttttggcata ttcttaggtc tagctaattt catatcatca
    13441 ttgattagag tacctattcc accatcgcta aataccataa attgacctga acttgccaac
    13501 actggtaaat aagttttttc tccattcttt tctataactt ccttacctaa ctttgtagct
    13561 ttttcatcat acttctgtag ccaactttta gcttcttctt ctttgtcaaa taactttgaa
    13621 acatcagtta acttgcttct ccagtcattt gcataatctt tcatcataac tacaggagct
    13681 atttctttta attggtcata tattttttct tgtctttgac tcattattat taaatcagga
    13741 tttacttcta atatagcttc catatccata gtatccatca ttgagtgccc aactatcttt
    13801 acacctttaa gctcttctct tatgtaactt ggtaatttat cagtttcata tgagtctaca
    13861 tttgctgttg caacaggctt ataacctgct aataatagtt cttcactact tcctgatata
    13921 tcaactattt ttttaggatt tgatggtatc ttaacctctc ctttaacaga ttgtacaact
    13981 cttgtctctg atttatcttt atcctctgta ttcttatctg aacatgctgt aacaagtact
    14041 aatgtagtta taatagatat aaatattgca agtgatttta tttttttcat gattacttct
    14101 cctacttgtt tattatactt acatgatatt gattatcata agcacatatt tattatagca
    14161 tttttctatt tttagtcaat attattttgt aaaaggataa aaattcctaa ttaatcttaa
    14221 ttatatatag cttagataag gttttcttat attaaacagt atttttttaa agaattggac
    14281 acaaatacaa tgacttttag gttaaacaat gataaaatgc aatttataga taatgaattt
    14341 agtaaggagg attcaaaaca tggtacattt agtatattgt gataatgcag gaaaaaaagg
    14401 agaaaaggtc ttagataaaa tcttggctgg aacaaaaaca atggttatta gaggtgctgc
    14461 tggtagaaaa ataccacata gtagagtatt tgaagatgag ttactttatt ttatggaaaa
    14521 aggaactgca aaaatatctg caaaagccac tgttaaaaag gtagaaaact atgttaaatt
    14581 aacagaagat gaaatttcta aaattttaaa tgaaaatcaa ccaaaattaa atttatctga
    14641 aaagcaaaaa gttcgttggc ataaaaaatg tttatgctta gttgaatttg aaaatgttga
    14701 agaaataaca cctcttgatt ttgaccatca aggaaatatg gatgattggc ttattgttga
    14761 aaaaattgaa gatgtagttg ttggtactag tataccttac aattatgaga agtctaaatt
    14821 ttaaagtatc aataacataa tactatattg ctaaactcta cttaaataaa aaaagagtgc
    14881 atttaataat tataaaaatt atttttatac actctttttt aataataatt ttaaattact
    14941 tactttctaa tctttcatat acatctataa attcttctgc taattgttga aaattcatag
    15001 ctgtcattaa atggtcttgt gcatgaataa gtataacact tatttcagtt ttattaccat
    15061 tagcttcatt ttgtatcaat tctgtttgga atttatgagc tttatgaatt gattctttcg
    15121 acttttcaat acattctcta gctttatcta tattcccgct ctttgcttct tttattgctt
    15181 caaatgctag ccctttcgca tcacctgcaa atccaattat tccaaaagat atctctatca
    15241 tcttctcatc catttattcg acctcctaaa tatctctata gcttgatatg actaatccat
    15301 tcttttctaa aaattctttg acttttttag atgtaagtat cttgtgctct ttcgttctat
    15361 caatagcata agatgtagaa tttaaaatat aatcatctaa aaaagctggg tgagacataa
    15421 tatccactac atcatacttc attatttcgt ctatattttt tataaaatat tcttcactta
    15481 cattttcctt ataaaaacta tctattaaag gaactacttt agaatactct aaattatatt
    15541 caaatccacc tcttattgga agattatact tggttacaat cttctctatt actggcttta
    15601 aacttactaa agtatggata tgatggtgac tatctaaatg agatatatct attccaagcc
    15661 cttttaccct atctatttga gcacaaagtt ctctataaat ctcatcacaa tccatttttt
    15721 caataatttc gtctgtaatc ctcctaataa aaaaaccgtc tttatcaaca atagttttta
    15781 agttagataa taatggtcta ccacaactta aagtcatatg aactccacaa tttaaagttt
    15841 tattttcttt aagtaagcct actccatgtt ctaccccagg catatttgcc atcatagaag
    15901 tagacctaac tataccatta ttatgagctg aaattattcc atagttcaca gcttcacaat
    15961 atccaaaatc atctgcattt attataattt tagtcatacc ataacctact ttctatagac
    16021 aactatctat taaatctagg aagataatct ttatgagctt ccaacatttc gtctaatata
    16081 gtttttgcta agtcatcaga tggaatcaat gggtttatac aaagtgccaa aagagcagat
    16141 tcataatcgc catatacagc tgctttagct gccaatactt caaatgattt aatttgactt
    16201 actaatccat gcacagaatc tggtaaatat cctatactta ttggttttgg accatttttt
    16261 gttattacac tacttacttc taccgcttgt tcatctttaa aatctcttat agaaccctta
    16321 tttaatgtat taactacttg gatatctttt ttgtcgttgt atatagaact tattaaatta
    16381 caagcagcat cactatagta agcccctcct cttttttcaa gttgaggtgg ttttacatct
    16441 aatttttcat ccttatataa ttcaaataat tgttcttcca attctttaac tacttgacct
    16501 ctagcttttc cctctttaaa ctctctcaac tcatcctcaa gcatttcttt agttttatag
    16561 tagtatctat gatatggaca aggtattgca cccaaagatt ttacaaattc agcattaaaa
    16621 tcaattgcct ttatattttg catacttata tctgctttga caaatttatc aattgcttct
    16681 tttgtaacat cttctccatc taaagataca tttagaccat aaaccatatg attaagacct
    16741 gcaaaatcca tacttattct gtcactttct acattaaata gtttggcaac atcatttttt
    16801 aaatgaattg gcacgttgca tagacctata tatcttctaa aattagtata tttgaataca
    16861 gcctctgtta ttattcctgt tggatttgta aaattcacca tccatgcatt tggacataat
    16921 tcttccacat cttttattat atcaaatata actggtattg tgcgaagggc tttaaataaa
    16981 cctccagcac cattagtttc ctgccccata actccatgag ataaaggaat agtttcatct
    17041 tttattctcg catctaaaag acctactcta agttgtgttg tcacaaagtc tgcatctctt
    17101 agagcttctc ttctatctaa agtaagattt attttcatat ctactccagc tttttttacc
    17161 atcctcttag ctagatttcc aactatctct aatttttctt ttccagcttc tatatctaca
    17221 agccataatt cctttacagg taactcttca tatctattta taaaaccctc tacaagctca
    17281 ggagtataac ttgaacctcc tccaattgta actattttta gtcccttttt catctcatta
    17341 taccatcctt acctataatt taatatactc tacaaaaccc catacattta tttaaaactc
    17401 tatacattta tttaccgaaa taaaactacc tagttgttaa ttttcaattt attagcttcc
    17461 attttttcag acgctatgac aaatggaata tatattaata ttgataatat taaattaaat
    17521 agtgctaaag ctccaccagt ccagtcacct gtagccattg ctccacctaa tataggaggt
    17581 gttacccaag gtattttagg taaaattact ggtgctacta atccaaaatc tattgctaag
    17641 tatgatacag cactacatat tactggtgca agtacaaatg gaatcataaa tataggattt
    17701 aaaactattg gaagtccaaa tatcaatggt tctcctatgt taaatattgc aggtggacca
    17761 cctaatacta tcatctcttt acgtcttctt ccagctatta ataaagctac tattaatccc
    17821 aataccattc cagaaccacc taaatataca aatgcatcaa agaatgcacc tgcaagtaca
    17881 tggtatcctt ccgttgctcc tgcttgagcc aaggcagcat tttctccacc taatttcata
    17941 agtatagttt ctgtaaaagg aagtgctata tttgcaccat gaagtccaac agtccaaagt
    18001 atatgaatta taaatgcaat aagcattgta gaacctaaac tatcagctac atttgatagt
    18061 ggtgctccta aataagtatt taaaatatct gttaagaaac ttccattaga aagcattttt
    18121 ataaatattc ctattacagt aaatatcatt atagttaaca ttcctggtat taactttgca
    18181 aatgatcttg aaactgctgg tggaactcca tctggcattt ttatagttaa tttctttacc
    18241 ttagataatc ttacaaatat ctcagttgat aataatccta ttaatataga agagaataaa
    18301 gctgctgtac ctaagtaagt ttgttgtatc attccccatc cttcaactac tgtagttcct
    18361 ccctcaggaa ctattttacc aatttgtgga gccattataa aaaagataga taaagctata
    18421 agcccagatt gtaacccatc actctcataa gattttgcta gaaaataagc aacacctatt
    18481 actaagaata ctgccattgt acctattgct ccccaccaaa ggtcgcctcc aaatgtggtc
    18541 cagttttctc caaatatact tgccatcaag tttttgtatg cttccaatgg taagttattt
    18601 atcaatgttc ccaatgcacc taccatagtt ataggtatca ttactataaa tgcatctctt
    18661 actgctacta agtgcctttg agctcctatt ttagcagcta caggcactat atatttatcc
    18721 ataaatgaca tgaatttttc cattttacat cctccagtac aactttattt tgaactattt
    18781 aactctacag ctctatctaa cacagctttt ccattacatc ttccataatc catcatattt
    18841 ataacttcta ctggaatatt atatggtttt gccatttcag ttgcttttcc taatacatat
    18901 ctaacttgag gccctaaaag taagacatca caattttcta tttcattttt taagttcact
    18961 tctgatatag cccatatttt tgcttctata ccgttttcct ttgctgcatc ctccattttt
    19021 gtaactaata aactagttga cattcctgct gaacaagcta acattatttt tatcattttt
    19081 tcccctccaa attagattca ttaaatattt tttaagtcat aagtttttta aactatacat
    19141 ttaaaatatg cttttctat
//
LOCUS       DAEOSP010000058        18529 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.058, whole genome shotgun sequence.
ACCESSION   DAEOSP010000058 DAEOSP010000000
VERSION     DAEOSP010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18529
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.058"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..517)
                     /gene="yqeC"
                     /locus_tag="KRH54_001675"
     CDS             complement(<1..517)
                     /gene="yqeC"
                     /locus_tag="KRH54_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBG8579341.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSLINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTV"
     gene            complement(700..1977)
                     /locus_tag="KRH54_001676"
     CDS             complement(700..1977)
                     /locus_tag="KRH54_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG8579342.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            complement(2116..4422)
                     /locus_tag="KRH54_001677"
     CDS             complement(2116..4422)
                     /locus_tag="KRH54_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG8579343.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            complement(4415..5209)
                     /locus_tag="KRH54_001678"
     CDS             complement(4415..5209)
                     /locus_tag="KRH54_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG8579344.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDKKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            complement(5316..6674)
                     /locus_tag="KRH54_001679"
     CDS             complement(5316..6674)
                     /locus_tag="KRH54_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG8579345.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            complement(8020..8985)
                     /locus_tag="KRH54_001680"
     CDS             complement(8020..8985)
                     /locus_tag="KRH54_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579346.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEITVMKEDMSEVKQEINVLKGNMSEVKQEMIVMKEDMSEVRQEINIMKEDMSEVRQ
                     EINTMKEDMSEVRQEMTVMKEDMSEVKQEINVLKEDMSEVRQEINIMKEDMSEVRQEM
                     TVMKEDTSEVKQEINVMKKDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNG
                     IKDEVSIANEKLYGIEIKIDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIE
                     HNIAYIKGDTNSIKEDIAEIRRDLNLVELATSKNWSDIVKLKSVK"
     gene            complement(9215..10300)
                     /locus_tag="KRH54_001681"
     CDS             complement(9215..10300)
                     /locus_tag="KRH54_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBG8579347.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(10336..11649)
                     /locus_tag="KRH54_001682"
     CDS             complement(10336..11649)
                     /locus_tag="KRH54_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG8579348.1"
                     /translation="MIAIKSKYLISSADEIYANSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNSP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETILASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(11650..13941)
                     /locus_tag="KRH54_001683"
     CDS             complement(11650..13941)
                     /locus_tag="KRH54_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG8579349.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(13931..14725)
                     /locus_tag="KRH54_001684"
     CDS             complement(13931..14725)
                     /locus_tag="KRH54_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG8579350.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(14764..15210)
                     /locus_tag="KRH54_001685"
     CDS             complement(14764..15210)
                     /locus_tag="KRH54_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG8579351.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(15282..16688)
                     /locus_tag="KRH54_001686"
     CDS             complement(15282..16688)
                     /locus_tag="KRH54_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8579352.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(16706..18076)
                     /gene="hydA"
                     /locus_tag="KRH54_001687"
     CDS             complement(16706..18076)
                     /gene="hydA"
                     /locus_tag="KRH54_001687"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG8579353.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGEDLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(18209..>18529)
                     /locus_tag="KRH54_001688"
     CDS             complement(18209..>18529)
                     /locus_tag="KRH54_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG8579354.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKS
                     VYGK"
BASE COUNT         5563 a         3613 c         1973 g         7380 t
ORIGIN      
        1 ttactgtaga actactatta atactacttg tatctaaatt tgctattttt ttaaatattt
       61 ctactctatg tatattctct tcattaatat tcattccaaa tgatgttata tctaatatcc
      121 ctatggtttt tgtagtttta tgatatacta ctggttcttt agcattccaa cctttaagtt
      181 tttttctctt tgagccatct ccctctatta aaactaaatc aaattggtct acaatttcat
      241 ccaaaataga ggaatcaata cttactaatt tattttcatt atttatataa ctgccacaga
      301 ctgttattcc atgacaaata gatggaataa cagttccatc tattgtaata attatattgt
      361 cataatcgtt tggtacatat attttagttg ttgttgtaag gagaactttc aatttatctc
      421 tataaaaatt tgcaaaatag tttataagcg aagtttttcc tcctgctcca actacagtta
      481 ttatttcatt cttttctatt atatttgata gttccacact ttcacctctt ataaccagtt
      541 ttctaatctc ttaattatta tagttagacc tttttagtaa aagtgatttt tgaaattcca
      601 taaactcgaa agtaaataga ttctgttgta aacaacaagg cggatccaat tcgccgctaa
      661 gctgtaagct tacgctatca gtttatcttt taaatataat taagatattc ttactctctg
      721 tggttgcttt aatatttgag gattattctg taaattagat aaattaacta cttgtgattt
      781 ttttagtata ttaagactcg catttacatc tgcatttata atgattccaa cactggattt
      841 gtactgtcca cgcgttattc tttccccttt aaagtcatat tttttaacat tgtccacatt
      901 gatatcaggt agaaaatcat cagcaaaaaa atctgattta ctagtatagc tttcttcttg
      961 cctaataaaa ttgatatttt ttattttaca cagatattct aattttcctt ttatctctcc
     1021 aaatgggatt cttataaaat ttctatatat ttttctctcc atattatcac ttttttctaa
     1081 cttagcatta tatcctacaa ttaaatttcc aatattattt tttacacaat aatcaattat
     1141 ataaaaacag gttttattta catagtcttt aacttgatta tttctcttat tccaaacttt
     1201 aaattgtttt cttgttattg cagttatatt cttttttaat tttaaggctt ctattttatc
     1261 attctgtttg tttgcccact gattcacaga ttttagcctt tttccatcta tgataaaact
     1321 atcacctaaa ttggtaacac atgtacataa attttctaaa cccaagtcta tagctagtgc
     1381 atttttttga tttgaaactt cctctgattt tggtatttca taacaatact gaatttcaaa
     1441 gaacctagct ttatgtttag gtattattct tatttcttta atttttttct ctagtatatt
     1501 ttgtggaact tttatactta ctttcccata tagtttttta aatgctggag acataggtac
     1561 atttaaaatc ccatcttctt tgattcttat ttgacctatt attatgctaa aaaatccttc
     1621 tttatctaaa tatttaggtg attttatccc tctaaagtca tattttcctt ctttggctaa
     1681 tttaattaat ccaaaaaatg acctaaacga actgtctact tcttttaata tttgttgcgc
     1741 tatattacta ttgagaagct tatagttctc attttctttg catacatgat aattagcttc
     1801 atagttaaga tgttctcctt gattgaaata atgctgtctt acattatatg taccaacatt
     1861 atataagttc ttagctaaga agcacatttc tcgtagagct tgatattctt ttttagttaa
     1921 gttttttaat tgttgtctct gagttccata gtgtatattt tttattctgt ttgacatgaa
     1981 ctcacctcct ttagaatata ctctactaga cattatatac ggtattttta caaaacacca
     2041 tatataatgt ccatttaatt actttatttt acaaaataat ttaaatttac ttgcttttat
     2101 acatcttatt atttattata attgtttatt attatttttc atacccatat aaactttttc
     2161 aaaagtcata ggaagctctc ttaaacgaac tcctgtagca ttaaatattg catttgctat
     2221 agctggtgct ggtgtatgta taactatttc tcctaatgat ttagcaccat aaggacctcc
     2281 tgggtcgaaa ttctcagcaa aatctaccaa tatatttcca acttcttttt ttgtaggaac
     2341 tttatactgt aggaaactat ctgttaataa tctaccatct ttatcaaata tagggtcttc
     2401 atacattgca aaccctatac ccatcatcac accaccttct acttgtattc tagctaaagt
     2461 tggatttata actgttccac aatcaggtac acatgcaaaa tttatgactt tatattcgcc
     2521 tgtattttta tctaattcta tctctgcaaa tcctgctata aatggtttta tttttttcaa
     2581 catgccaaaa gattctgttg cttccaaaac tactggtaca tcatcactag caaatcctat
     2641 tcctccaaca gattctctac ccaattcctc taatagaaca aacttgctat tatcgttttt
     2701 aacaaaaacc ctgtcttttc ccaactctaa atcttctttt ttaaatccta gctttgactc
     2761 tgcaactgaa agtaatatat cttttaattt atttgctgtc tttattgttg catttcctgt
     2821 aaccgttgtc gtacaagaag caaatgctcc tgtatcatag ggacataagt ctgaatcacc
     2881 tgtataaata gaaattctat ctacagtagt atttaaagct tccgcagcaa tttgagcttg
     2941 aattgtatca gccccttgac ctaagtcagc aagaccagat aatagtttat atgtgccatc
     3001 ttcatccatt cttatagtga ctgttgccat atctacacgc ggtataccag aaccatgatt
     3061 tgctactgcc atacctactg ctcttacttt gttgtttcct atatctttac atggatattt
     3121 atcatcccaa ttaattaatt tcttgccctt ttctatacat tgtctcagct cacaacttaa
     3181 tatttcgtct tcagctgctt ttccattggt atacctatct attatatttt taattctaaa
     3241 ttcaagtgga tccattccaa tcttatgtgc caattcatca atagcactat ctattgcaaa
     3301 tattccctgt gtagcaccat accctctaag tgctcctgct ggtaacctat tagtatatac
     3361 tgttctagca ttaaatctaa cagcatcaat atcattatat attggtaagg tattgtgtcc
     3421 agattcggaa gaaacagaga caccattccc accataagca cctgtattgt ttaatgactt
     3481 tagctctatt gcttttaaat ttccttcttt atctgcacca actttaacat caaaaaacat
     3541 ttcatgtcta ctattagaac ctacaaaagt ttcacttctt gtaaatacaa attttgctgg
     3601 tctaccagtt ttcatagtaa caaatgcagt aaatatatct gttgctgcta tattctttcc
     3661 tccaaatcca ccacctattc taggtttaat tactcttatt tttgaagatg gtatacccaa
     3721 tacttttcta agtattctct ttacattgtg tggtgattgt aatgatgaaa ctacaacaat
     3781 tctatcattt atatctttat atgaatatga cctatgagtt tccatcatgc aatgcgattg
     3841 actttgggtt ttgtatgaag attcaaccac aaaatcactt tctttaagct tagcttcaac
     3901 atctccaaat tcaaaaaatt cttttgatac tatattattt tttgcatcca atccaaaagg
     3961 atatggctct actatgtctt tttctggatg tataacaact tcatttcctt ctgcttctct
     4021 tgcatcaaac acaggcttta aaacctcata atctaccttt atcattttta aggctttaag
     4081 tgccgttttc tcatcttcag cagccactaa agctacttca tctccaataa atctaactgt
     4141 atcttcaagt aaaactctat cataaggtga tgcttctggt tctgtttgac ctggcacaca
     4201 atatctgacc tgtggtacat ctttatacgt ataaataccc acaacccctg gaattttttc
     4261 ggcttttgat gtatctatat tctttatctt agcatgagca tgtggacttc taagtagttt
     4321 taatgtcaaa acatctttat gcataatgag gtcttcagta tatagaggtt ttcctgtgac
     4381 aattgctgta ctatctatct ttcttatccc cttattaatc attatacatt ccctcctcaa
     4441 tagcattata catcctttta actaatgcac ttgccatatc ctttctgtac tctttactag
     4501 ctctcatatt acttccataa gtaagttcct tactagcaac aatagatgcc ttatctatat
     4561 ctttttcttt atttaatata ttactcgctt caagtgctaa tctagctttt tgaggtcttg
     4621 ccccaattgc tattttgtaa gtatcatttt cttttagcat tgctgaattt aatacagaaa
     4681 aatctcctgt gcattttctt attgagtcaa aaacagctat tgcatctttt tttggtagta
     4741 ttacttcgac caatatatcc ctacttaatt cactatccaa aaactcactt aactctagta
     4801 ttcctttatt gtataatctc accttcgctc caacaaccaa aagcgctggt attaagtcag
     4861 aaaagccata ctttgaaaat acacttgcac caattctagc cccttgtcta aattgaacac
     4921 caactatatt agaaacagcg cttgaaagta ttccactaca gtaatttttt atagttttat
     4981 caatttctaa acttctaagt gaagtatctg cacctatcaa aatatttttt ttatcctctt
     5041 ttatataatt tagatttata tcttttaaat ctatcgctgt tcctatattt ttattactta
     5101 attttataaa tccacatcca cctaaaataa cattattttt tttagaaatt aaaattttat
     5161 atgcttcttc taaactctgt ggaactgaat attcctttat agtaaccatc ttatcccccc
     5221 atcattaact tattaaaaca caaaagtttt taataaatct tttatttata tttataattg
     5281 tagtatattt aattcacata tattaaacct ttacattata aacttttatt tttatcctca
     5341 accttatcac ttttatttcc aggaaatttt accatatata aagcttgaat tattatggct
     5401 accattgctc ctgaaactac acttgaatct gcaagtaatg tctctataaa ttttggaaat
     5461 ttttcaagaa cttgaggagc taccatgaat ccaataccag aagccataga aagacctaat
     5521 atcatgctat tttcttgatt aaatccatcc atactaacaa gtcttaaacc agatgttgta
     5581 agagttgaaa atataacgag tgttgctcct ccaaccacac agggaggaat tgttcttatt
     5641 aaattagcta ataaaggcga tattcctgcc aaaaataatc caattcctcc aatagctagt
     5701 acaaatttac ttacaacctt tgtattggaa acgattgcag aattttgtcc aaacatcccc
     5761 attggagttg cagaaaataa tgcacctatt gtagagagta ctccatttcc taaaacagca
     5821 gaagaaagtt cttcatctgt tacttgtcta ttaagccctc ctaccgcaga aagtgtacaa
     5881 gcacccatta tatcagctat ctcaactaaa tatattattg aaaacattac aacaacttca
     5941 agtcttatat ctagcccaaa agcaagtggt tttggaagtg caaccaaagt aaactcctga
     6001 attgaagaaa aatttactat acccaaaatt aatgataaaa tgtatccaaa aattatgcta
     6061 actaatattg atgcatcttt aaataatcct tttccatact tatttaatcc taatatcata
     6121 agagatacag atattccaac tataaaatta attggattcc caaaagtctc attgcccata
     6181 cccccagcta agttttttat agctattgga aaaagtccaa gacccataca tgcaactatt
     6241 gttcctgata cagctggagt aaatataaat cgtatttttt taagaccaaa accaattata
     6301 attattacta taccagctat tatttgagag ccaaataata ctggtagtcc atacttactt
     6361 gcaacagata ccccagctcc tataaacata gctgtagctc ccattataac tggtattcct
     6421 cctcctaatt taaaaccttt aaacaaaggt attggatata attgtaataa agttgataat
     6481 cctgcaaaaa ctaatgcggc ttgaatcaat aatgttgaca tcttatgatt aagtccagct
     6541 gcatttgcaa tcaatatcgc tggtaaacaa ctacctatta ccattgccat taagtgttgt
     6601 aaccctaaag gtattgcttc ctttagtggt ggtattccgt ctaattcaaa ttttgatgta
     6661 ctttttgctt tcataataaa aactcccgtt acataaaact tattgaatta tataaatact
     6721 taaaaatcta atatattaaa ttaatgatgt tcataaatat caactatact ccaacatata
     6781 tttaagtttt tagtcttaaa aaacagatgc aataaattta attattaaca aatacacgta
     6841 ttctttaatt ttgtatacat tatataccat gctttacaaa tataagagta ttatagctat
     6901 gaatttttaa ttttttatag taaatttaat tcaatttaaa tctttcaatc ttaatcatta
     6961 taagttataa gttttcataa gtatttacat gtttcaagtt ttaaacattt acctataaac
     7021 gctactttat agatgtcact tcaaatccag tgacctcata ctttcatatg agcgtagact
     7081 atttcttcat cttcagcatt acctgctaag agcaacccgt ttccactatc aatcgcttat
     7141 agtgtactct ctctcgagat agtcgtttga ccttcctatt tctaggcttg gcgaccaaat
     7201 gtccattttt aagtacttag gctctcacca tataccatcc tcactattgt ttctggtttt
     7261 caccacattc ataaaagttt ctttctatct tttattgtag ttgcaaggct ttaggattta
     7321 ctggttttaa agttgtgtcc tacatagatt tctctatgta cggggccaaa agtacaattt
     7381 atttaacata ctttgcttta ccccctgtaa ataatattat ttactctttc actgtttttg
     7441 ttattaaatt aattaaatat caattaatct aagcataaaa taattattta atttaaactc
     7501 aaaatattta tgaatatttt ataatataat tctcataaga cagaaaaact taataagcta
     7561 tgtatctcag ttgtaccaat gtactctcct ttactatatt tgtatctgca acataaaata
     7621 tacaagtttc tatctattta tagatatata tatctataag caaaatagta atattatttt
     7681 tatagtaaaa attttattca tattactatt tataattttt atagagcaat tttagtgcca
     7741 atatttgcat acatttgtat agtataaaat ttttatgttc tcattgataa aattttactt
     7801 atttcaagcg ttctattaga ttttatcatc taaattttag gttttatttt tataaattaa
     7861 ttgtatttac ataaaaaaaa agacctaaaa caaattttta acggtctttt ctcaattttt
     7921 tattttcatt tctaaaattt ataataaata gtaagaacac tataaattct tttatataaa
     7981 attcttagtt tgtggatgat aaattagtga attaaaatac tactttactg attttagttt
     8041 gacaatatca ctccaatttt tactagtggc taactctact aaatttaagt ctcttcttat
     8101 ttcagctata tcctctttta tagaattagt gtcacctttt atatatgcta tgttatgttc
     8161 tatattatca tgagtagctt tatttatttc agaattatgt aataaacttc ctaaaatatt
     8221 attctgttct atctgtatct ctttcattga tttatcttca cattctagac tatcaatctt
     8281 aatttcaatt ccatataatt tttcatttgc tatggatacc tcgtccttaa ttccattcat
     8341 atcttcatta atgttattta ttttattttg cataaagttt acttctgatt taacttcgga
     8401 aacatcttct ttcattacag ttatttcttg tctcacttct gacatgtctt ttttcattac
     8461 atttatctct tgttttacct ctgatgtatc ttctttcatt acggtcattt cttgtcttac
     8521 ttcggacata tcttctttca ttatatttat ctcttgtctt acttcggaca tatcttcttt
     8581 taatacattt atctcctgtt ttacttccga catatcttct ttcattacag tcatttcttg
     8641 tcttacttcc gacatatcct ctttcattgt atttatctct tgtcttacct cggacatatc
     8701 ctctttcatt atatttatct cttgtcttac ctcggacata tcttctttca ttacaatcat
     8761 ttcctgtttt acttctgaca tatttccttt taatacattt atttcctgtt ttacttccga
     8821 catatcttct ttcattacag ttatttcttg ttttacttcc gacatatctt cttttaacac
     8881 atttatttct tgttttactt ccgacatatc attttttatg ctaactacat cttcatttat
     8941 agtatataat aactctaaaa tcttctcttg cacttgcatc accatcccta ttaattatta
     9001 taacatttta cacaaatgct atacaatgtt aacatatata tcagaattat taaaatttta
     9061 attaaacaaa attgattcac tttattactt caaaactaat taatatctaa aataactaca
     9121 aataaacaaa aataaggtca acaacaaaaa aggcttatct ctttgagata gcctttctta
     9181 tttttattta aaactttgtt ttttataaaa taatctatac attcagctct gaacatgaaa
     9241 tttttcttcc aagtgctttt ggtttacaaa ctgatataca aagtccacaa tatctacact
     9301 cttcttcatt caattccatc ttatcattca attttctcgc ttgatatgca caaactctct
     9361 cgcacatctt acatttagta catttttctt catcataagt aaactcaaag ctaagtcttt
     9421 cattaacatc tcccatatga ttatgtacta taccacaagc attttctata gtattatatc
     9481 ctaatctatt aagattagat tttaaatcat catgtatttt agatattact tgtgcgcctt
     9541 ttaaaattac tactgaacat ataccaacaa agtcagcacc tgccataatc atttctaagg
     9601 catcatctcc acttacacat ccacctaagc caattattgg tatattaact tgttttttta
     9661 tttcatatat tctctgtaaa gttattggct tgatagtttc tccacttaaa tatcctattc
     9721 catttccacc taataaaggt tgccctgttt ctatgtctat tctaaatgct ggtcctacag
     9781 agtcacaagc agttattgca tctgctcctg cttcttctaa ttttttagca aaatcgccta
     9841 tctcatctat cataggagga agtttaacta ttacaggtat atttactctt tcctttgcat
     9901 ctttaagcat tggtatcaaa tctctatagt cataagatac taattcaata aagtcagctc
     9961 ctgcatttgc aactttctct actaattcgc tactttcttc aattgtatga cctattgaag
    10021 ctatacagac agtcccatct cttttcattt gtggtatttc aaaatctatc cattgctctg
    10081 gctcaaaatc tgtccatttt tcattattta aaagagcatt agctgtattt ctaaccatat
    10141 gtggtgctgg atttatagct ctttcttttc ttatagtctt agtcacaact gcccctgctc
    10201 ctgcatcact taatatcttg catccagctg cactatatgt taatggtcct gaacctatta
    10261 taagtggtga ttttaattct ttacctaaaa aattatacat tatctgcttc cccttttcat
    10321 atatatttat attcgttaaa atttccaaaa ttcacaagca acttctttca tttctttaac
    10381 aatagctttt ttatctaaat caactaactg tttttctttt aaaatatgtc taccatctat
    10441 gtatacattt gatataaatt cttttttacc ctgaactact atctggtcaa atatattatc
    10501 aagagtcaca ggagttttaa attcatcttc cataactatt atatcagcct tattaccaac
    10561 tgctatttta ccagaatttt gaagtcctaa tacataagca ccatgctctg ttgccattct
    10621 aaatactaaa tttgctggca ttactgatgc atcttcttca actgatttat gcattaaaaa
    10681 tgcacctctc ataacttcaa aaaaatcatt tatatatcca tcagtaccca gtgccacttt
    10741 tatgcctttt ttaatcattt ttggtactgg agaaaaacct ccacctactt cacagttgct
    10801 aagaggcata tgaactactt ttacaccttt ttcttttaat atatctattt cttcatcatt
    10861 tacttttaca cactgagatg ctaatatagt ttcatccaat atatctaaat cattatagta
    10921 gtcaactgct tttttgccaa aatgtttttc agcatagttt ggctcatata tactctcaca
    10981 taaatgaaat tgcattggtg aatttaattt ttttgcatct tctttagctt tcttaataaa
    11041 tctatcagaa catgtgaagc ttgtatgtgt acacattatt ccatttatta atttactatt
    11101 ttctctactc cactttatta aattagaatt ttcatctaaa catctaagtc cattttcaaa
    11161 gcttattctt tcacaacttt ctaaggataa aatcgctttc atgcctattt cttctagtat
    11221 ttttccttgc tcaattaaag ttccttcttc tgtatttgga gcttctaggg tatcacaaaa
    11281 agctaccaca ccagattcta taagttctat tgctgatgct ttagttgttg ccttcacttc
    11341 tttcaatcca attctatttt ctataaatgg ccaccaatag tcatttaaaa aaccttcaaa
    11401 gtcagtaaaa tgtacatttg ccggtattcc tctagaaaga actccatatt gatgcatatg
    11461 accattgacg aatcctggca ttattatagc atctgattta tctattattt cttcaatatt
    11521 tttatatttt atttcaactt cttcatttgg caatatatct tttataatgt cattttctac
    11581 tacaacagca gaatttgcat atatttcatc agcagaagat attaagtatt tacttttaat
    11641 agcaatcatt tatttacctc cctgatttaa ttttttcatg gccatataaa ctttctcaga
    11701 agttatagga agttctctta tacgaactcc tgtggcattg tatattgcat ttgctattgc
    11761 tggtgcaggt gtatgaataa ctatctctcc taaagattta gctccatatg gacctgttgg
    11821 ctcataatta tctacaaaat caacaattat gtttcctata tctttttttg ttggaacttt
    11881 gtattgtaat agattagaag tttgaagctt tccatcagaa ccatgtctag ggtcttcata
    11941 cattgcaagc cctatacctt gagttacacc accttcaact tgtattctag caagtgcagg
    12001 attcataact gtaccacaat ctgtagaaca agcataattt attactttaa actcccctgt
    12061 tgttttatct aactctattt cagcaaatcc tgctaagaat ggtttagcac tctcttgtat
    12121 accataagat tcagatgaat ataaaacttc ttgatttcca ccaccaacag attcttttcc
    12181 taattcactt aacaaaacaa acttctctac atcttcacta taacaaactc tgtcttcata
    12241 tagtattaaa tcatctattg gtaaatcgag tttcttctgt gctactttta atatcttttg
    12301 ttttaaactt tcagcagatt ttattgtagc atttccagtt acataagtag tacaagaagc
    12361 gtatgcacca gtatcatatg gacacatatc tgtatctcct gtataaacgg aaattctatc
    12421 aatagatgta ttcaatgctt ttgcagctat ttgtgcaagt attgtgtcag aaccagttcc
    12481 taagtctgaa gacccagaaa acaacttata agttccatct tcatccatcc tcatatttac
    12541 tattgccatg tctatgttgg ctataccaga accatgagtt cccacagaca ttccaacagc
    12601 cctcacctta ttgttgccaa tatccctaac agggtacttt tcatcccatc caataagttt
    12661 tttacctcta attatacatt catcaagtgc acaacttctt attggaaaat tcattattcc
    12721 gccttcagtt tgttttttta taatattctt taactttatt tctgttgggt ccatattaag
    12781 tttatgcgct aattcatcaa ttgcacaatc aagtgcaaat gtaccttgtg ttgctccata
    12841 tcctcttaat gctcctcctg gaactaaatt agtatataca gttctcccat caaattttat
    12901 tgcaggtaca ttattatatg ttggaagtac attatgacct gattcagagc aaactgaagg
    12961 tccattatca ccataagcgc ctgtattgtt caatgctttc atatctatag cttttatgtt
    13021 tccttcttta tctgccccaa tttttatatc aaaaaacatc tgatgtctag tattagtcat
    13081 tgcaaaagtt tcttctctag tataaattag ttttgctggt ctttttgtca tccaagttac
    13141 aaatgatgca tatatctctg ttacagctat gttttttcca ccaaaacctc cacctattct
    13201 tggttttatt actctcactt ttgaaagagg tattcccagt gctttagaaa cctgtcttct
    13261 catatgataa gctgattggt ttgcagaagt tactactaat ctgccattcg tatctatata
    13321 cgtataagct ctatgagttt ccatcataca atgggcttgt gattgagtca tataagaatg
    13381 ttctactatt acatcacttt ctttaaactc tttttctaca tcccctttac ccattttttc
    13441 tctcgataca atattttttg tagcatccaa tccaaaatca aatggacaaa acaaatcatc
    13501 ttcttcatgg attaaaattt tatttccttc cgattctctt gcatccaata taggagtcat
    13561 tacttcatat tcaactttta taagtttcat agctttaata gcagcccttt catcttcagc
    13621 ggctattata gcaacttcat ctcctacata tctaactacg tcttcaagta ttaattggtc
    13681 atgaggtgat gcttctggat atgactctcc aaccattgaa tatctataat ttggaacatc
    13741 tttgtaagtg aatatgttta ctacaccttt tacctttaat gcatttttta tatctatgtt
    13801 ttttatcttc gcaaaagcat gtggacttct aagaagctta attgttaaaa catctttatg
    13861 aaaaattaaa tcttctgtat atattggttt tccagtaaca atggcttcac tatctatttt
    13921 tcttacctct ttatttacca tcacactcac ctccaatact attatacatt ctcactacta
    13981 aggcttttgc catatctttt ctgtattctt tacttcctct tatattactt ccaaatgaaa
    14041 gttcttcact tgatacttca ccagcttttt ctatatcatt ttctaaactt aatatttcac
    14101 ttgctttata ggcaattttt gctcttcttg gtctagcgcc tattgcaatt ttataaatac
    14161 ttccttcttt taacattgct ccatttaata ctgcaaaatc acctgtacat tttcttatag
    14221 aatcaaatac acctatagca tcttttttag gtaatataac ttctattaat atatcttttt
    14281 caagctcact ttctaaaaaa tcagctaagt tcataacccc ttttttatat aacttaacct
    14341 ttgcatctac cactagtaat gaaggtatta aatctgaaaa tccatactta gcaaatacgc
    14401 tagctcctac tctagctcca cttctaaatt gaactcctac tatattagaa acagcgtttg
    14461 aaattacccc attacagtaa tttttaataa ccttatttaa ttctaaagtt cttaaacttg
    14521 tgtctgctcc aattaatatg ctatcatcag tttcatttat ataatctaaa gctaagtctt
    14581 ttaaatctat tgctgatcca atatttttac tacctagttt taaaaatcca catcctccaa
    14641 gtattatgtt gttttttctt gatattagca gttcataagc ttcatctaaa ctttttggaa
    14701 ctacatattc ctttatagtt atcatactct ctccccatat caacttaatt ttcacttaaa
    14761 agtctaagtg tttaaatatt tatgtattgc tcttaattga cctttatatc ctgtacatct
    14821 acaaaggtta ccatttacat aatctataac ttcttcttct gttggatttt gaagctctct
    14881 tttcattgcc aaaaccatca ttataaatcc aggagcacaa aatccacact ggtcagcccc
    14941 ttcgtctgca agaaaatctc caaacttctt tgcttcatcc aagacaccct caagtgttgt
    15001 aatatgctga ccgtctactc tagcagtaag ataattacat gaaagcacgg ccttgtcatt
    15061 taccatcact gtacataacc cacatgagcc tgtatcacac ccttttttaa cacttacata
    15121 tccattttct cttaatgtat ctgctaaaaa atcatctggt cttatactta attctttttc
    15181 tttattattt atggtacatt tgataatcaa ataaatcacc tcaaaactca tatttttaag
    15241 ctaaagtttt actatacttt tctatgaata aattacattt attaataaag gctattccat
    15301 ttatgacctt ccattctatt ttttatagtt cctcttacac aaagtctctc agctgtatct
    15361 tgagcatctt ttaatgcttt ctcttcatta gctgtcaata tttttgaatc ttccattatt
    15421 atatttccat ctactataac actctctata tttttcatac tagaagaata tactaatgta
    15481 gatactggat tatgcatagg tatcgctttt ggtgaaagca ttggattaaa tattagtaag
    15541 tctgcttttt tacctatctc caatgaacct atctcatctt ccattccaat agctcttgct
    15601 ccatctatag ttgccaactc aagaactttt tctgctgaca ttgctaatgg gtcacactta
    15661 ttaactttat gttgaagtgc tgttaatttc ataagttcaa gcatatcttg agagttatta
    15721 ctagcggctc catctactcc taaactaact gttattcctt ttttaagcat ttctggcact
    15781 ggtgcaacac ctgaagataa gtacatatta cttgctgtat tatgagacac tttcatatcg
    15841 tattttttag taagttccat atctttttca gttagataca cacaatgtac cataagcaca
    15901 tttggtccaa gtattcctaa tttttctaaa acatctatgt catattgccc atgcaattct
    15961 tttgctgctt ctctatcaaa aggagtctct gatatatgta ctgtaaataa agcatcatca
    16021 tattctttta ctactctcca tagcatttca agcatttctt gagaatttga ccatattgct
    16081 gcaggagcta ctcctatttt gattcttcca ttttctgtat tatggtgttt ttcaaacaat
    16141 cttcttacat ctttttcaac cgtttctaca tcttgcatta ttccaggatg aactccaaat
    16201 tgagctcctg tattcataca tcctcttcca agtattcctc ttattcctaa ttctttataa
    16261 gcatctatta ttccatcaca aagacctggc ttactatgag gatacatgta gtcaaccatt
    16321 gttgttatac cacttctaag tccttcaata catccaagca ttgcagcatc ataagtatct
    16381 ttgggctcta aatagttagc tgctggaaat gtcatagttt ctaaccagtc tttaagtacc
    16441 atatcatctc ccaatccttt taataaagtt tggaataaat gattatgtgt atttataaaa
    16501 cctgggaata aaactttacc tttagcatct ataatttttt taacatcagt atatttactt
    16561 tctatctctt tagaatttcc aatatcagca atcttgttat cttttactac taatgcccca
    16621 tcaaaaataa cttctctctc tttattcaca gttactatta tggcattctt aataagtata
    16681 tccatcattc ttatttcccc ccgcattatt tagtaatttt tcttttaata tattgacctg
    16741 ttggatgttc tttaacatta caactttgta ctacatgatt tcctctcaaa aatacatctt
    16801 caactttaca tttaacctta aatccttcat atggagtata atctacattt tgtttttgag
    16861 ttttataact aattgtttct tctttattga tatttaaaat gactatatct gcatcacttc
    16921 ctacagctat ctcacctttt ttaggataca ttccaaatat ttttgcagca ttcgtagaag
    16981 taacttccac aaacttattt acacttattc tattttctaa gaccccatat gtatataaaa
    17041 gtgctaacct atgttcaaca ccaggtgctc catttggaat cttactaaaa tcatcaattc
    17101 ccaagtcttt ttgcccctta aaattaaatg aacagtggtc tgtgccaact gtttgaatat
    17161 ctccactaga caatccttcc caaaggtaat ttatgtcttc tttttttctt aaaggaggag
    17221 acattacata cttagcaccc tcaaatttat ccttattata taaattgtct tctaataata
    17281 gatactgtgg gcatgtttct actatcatat ctatattttt ttctctagcc atttttacag
    17341 tttcaagtga ctccctacaa cttaagtgga ctacatagga ttttgaatta gataatttag
    17401 ttatatcagc aagtcttgaa acagactctt tctcaactat gttaggtctt gtaagtggat
    17461 gatattttgg ctctaaatta ccattttgta tattttcttc tatcaataca tctagtaaat
    17521 ccccattttc acaatgaaat ccaactatac aacctaaatc ccttgctttc tttaaaaccc
    17581 tgtatattgt tccatcatct actttcatgc catcatatgc catatacatc ttaaaagata
    17641 ctatcccatc cgaaataagt ttttccatat gattaaatgt atctttattt aaacatgtaa
    17701 ttgtcatatg aaaaccataa tcacaataac aatttccact tgctttttta tgatatgctt
    17761 ctacaccttg cagtaggtct tccccctcat tggcttctgc aaaatctagt attgtagtag
    17821 ttcctccagc tatagctgct cttgttcctg tttcaaaatt atctgcagtt gtaatagaac
    17881 ctgcattcat atcaaaatga gtatgtgtgt ctattccacc aggaattaca attttacctg
    17941 tagcatcaat aaccttatca ccatttattt ctaagttatt tcctatttca actatttttt
    18001 catcttctat ccttaaatca cttatataag tcttatcaga ggtaattata gttccacctc
    18061 ttagtattgt tcccatatat acctcctaac ttttaagctt taaagtattt tctaagtatt
    18121 gtaaaacttt atttaaaatt gtattttcta aaggtgctat attcagcacc tctagactat
    18181 atttaaataa tttattgtga actaaatatt attttccgta tacagatttt aatattccat
    18241 agtaaccttc tgttcctata aataactgtt cttgctctat atactcatct attgtgtgag
    18301 caagattttc cttagaagga ccaaatccta tagtttttat tccagcttca cctgcatagt
    18361 gacttccatt cgtacaaaat gagtattgag ttatttctga atctattcca gcttctttta
    18421 atcccttata tgctgcttga acaaattcat cttcttcgtc atataaccaa cctgggaaga
    18481 atctttcact ttctatagta tttcctgtat agcaatcagc tttttcaac
//
LOCUS       DAEOSP010000059        18088 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.059, whole genome shotgun sequence.
ACCESSION   DAEOSP010000059 DAEOSP010000000
VERSION     DAEOSP010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18088
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.059"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            37..198
                     /locus_tag="KRH54_001689"
     CDS             37..198
                     /locus_tag="KRH54_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579355.1"
                     /translation="MAIVVIGIYLGFKREFSKLIGFLVVALVAVGLVFNAGGVKDVLL
                     ELFNKIIGA"
     gene            279..656
                     /locus_tag="KRH54_001690"
     CDS             279..656
                     /locus_tag="KRH54_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8579356.1"
                     /translation="MDYKVDDKNLDASMFISFVNQVWQGDYDMEQTRQALSKTLNITA
                     YDNKKLVGCLRILSDGYYFGTITELLVLPQYQKQGIGSKLLQLAKDNTPTMLYFGSQP
                     EAEKFYEKNGCQKSLQSYMINKG"
     gene            763..1059
                     /locus_tag="KRH54_001691"
     CDS             763..1059
                     /locus_tag="KRH54_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG8579357.1"
                     /translation="MNDIFKNMQAKIGCEYLSDLPSYKRKVWHEMKQLNLADYEEKQL
                     EDFSKYVFGMSYQTLKDVMKQQIGREEQCRKQGYWWKRKEQLTKKQHHTGSTCR"
     gene            978..1481
                     /locus_tag="KRH54_001692"
     CDS             978..1481
                     /locus_tag="KRH54_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG8579358.1"
                     /translation="MQETRVLVETKRTTDEETTSYWFDLPIEVAEFEEKLGVGAESGE
                     YRIIEKVLPYADEVHEHTSVYQLNKLDFMYRQLSSDMQEEYVSLLMVFENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFNFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            1499..2002
                     /locus_tag="KRH54_001693"
     CDS             1499..2002
                     /locus_tag="KRH54_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG8579359.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFSFPIDEEDVKEKIGLNEQY
                     EEYAVHDTDNFPIDIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GMCEIPY"
     gene            2123..2521
                     /locus_tag="KRH54_001694"
     CDS             2123..2521
                     /locus_tag="KRH54_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG8579360.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQTLNETITAVRSVNYVPDKIY"
     gene            2499..4949
                     /locus_tag="KRH54_001695"
     CDS             2499..4949
                     /locus_tag="KRH54_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8579361.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLREVAVQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEYQLPEKKLQKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALSTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSDSRGLLHVIDELRREDTPISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            4949..7144
                     /locus_tag="KRH54_001696"
     CDS             4949..7144
                     /locus_tag="KRH54_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG8579362.1"
                     /translation="MKERIQSAFTKKKMFRFLKITVFVVALSLILLSLLETVAHATGL
                     VDDTINTENLYSKYPLSNYQLDFYVDNSWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGQSIQTLAGVTSSGFSTSGFYVGFLLLIIL
                     VVGIYVAYTGLIKRETSKALHAVINFVVVFILSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SVSPEDEDGKTREEIVKTEIEDNDNNILTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNASDSQSMGRRIFRRP
                     YLFIRHRARRMERRMARAVSAGGISGGVAGALAGSTVSNARTTRNNPTKENRGNTTSI
                     MGQRAGSKIGAVLDTKNKVKDKANAVKENVKDMPTQTAYAVYSAKEKAKSSVSDFKRG
                     MVQEQQSRQTGRLEKQEQRKKNIANKRMELQKAQEARQARKADGSATTGATRPHERPA
                     TASKPSVEKVQEVKRPATTPTSKASEPVKTNVIKERPLSSGASDGKATQSAQPIHRQN
                     IEKVVSQETRQNYVKDRRTKVQQTQSVQKNQQTTEKNHNLVTKKGQKKK"
     gene            7141..8145
                     /locus_tag="KRH54_001697"
     CDS             7141..8145
                     /locus_tag="KRH54_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBG8579363.1"
                     /translation="MKLKHIALIGSLFPILFSIVLFFGVLISADSDDENSNFSSGITG
                     LNLSAEVLKHQPMVEKYAREYGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSYKNQGINDLNVAIQSYNYGGGYVGYVAGKGKKHTFTLA
                     ESFACEKSGGKKVTYTNTIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKIM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYGKAGISLPRTAQAQYDATQHIPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGYADLNSSYWQQHLIGAGRVK
                     "
     gene            8165..9067
                     /locus_tag="KRH54_001698"
     CDS             8165..9067
                     /locus_tag="KRH54_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG8579364.1"
                     /translation="MFKKSKKQTENVKEKKVRTVKVGTHKKTVIAFWVVLIVSVSFGV
                     YKNFTAIDMHTVHETETIQLRLTDTNGIENFVKNFAKSYYTWNNSKEAIEARTQAISG
                     YLTKELQDLNVDTIRTDIPTSSTVTDVIVWNIEQSGTDTFSATYEVDQQIKEGEQTTA
                     VKAIYTVKVHVDVDGDMVIIQNPTLAPTIEKSDYELKTPEADASVDADTINDATSFLE
                     TFFKLYPIATEKELAYYVSGNVLEPIGKDYLYSELINPVFTKDGDNVKVKVAVKFLDN
                     QTKATQVSQYELVLHKDSNWKIVK"
     gene            9211..9822
                     /locus_tag="KRH54_001699"
     CDS             9211..9822
                     /locus_tag="KRH54_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8579365.1"
                     /translation="MPKGIMLTQEQQEERREEIISVALQLIEKNGFQKTSMRKIAILA
                     NMGKSSLYDFFKTKDEIFVYAVEKEIEETIKKVHRIIADESSPEQCLRKIMLNHLGVP
                     KQYRTVLMWLNTESDYLEEDYRKRLKTARYAYQDIIKSVIENGMESGVFRKTNANLMT
                     RLLINSIISIIYTSRPSASPEKMLDETMDIFLHGIMNDGGELL"
     gene            9819..12386
                     /locus_tag="KRH54_001700"
     CDS             9819..12386
                     /locus_tag="KRH54_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG8579366.1"
                     /translation="MIYYTLPLLHKQATLEMVGGKGMSLSKLLTTGIPVPEGFHVTTT
                     SYKIFVEKNHIQPYINKLLDGIDTNNTSQLENVSTQIGMLFHNGEMPQEVSDAIKTAY
                     ARLGNIAVAVRSSATAEDLPDASFAGQQETYLNIQGENEVLDAVKRCWASLWTARAIA
                     YRVKNDIKQEIVALAVVVQKLAFSDASGVMFTLNPINGRRSEMIVNAAWGLGESVVSS
                     LVTPDTIVVDKNAERIVSYEVANKEIMTVRNSDGTEEIPTPEQLRKKHALTRNQVMRL
                     TQLGKKIEKYYEMPMDVEWALEKDKLYIVQARPITVLPPEWTLPEKDVIYTRGSLAEH
                     LPNPVTPLFATLGLEIVNRASALLWIDMFGKSAKKLLPENGAYTIINGYVYLSANSKP
                     LLIAVKSLSPRSLRRALTNSVARWETARKEFEDVIKQWEEKPMHMLNAHQIMEGIQTV
                     FYGACIYFTRIQLTLPAASISETLFTKFFQGAARRAGMTDTSVFLLGFDTIALQAEKN
                     LWSLSEWVKQNNTLNLYLQSNPTAKIAENFMSSVPPDEVSLEVWIEWKNRINQYFKEF
                     GRTAYEFDFAYSTPQETLTPTFESIKTFVEGKGESPFLRQSKFEKHRKQAEEEILQHI
                     GSSRKKLFLKLLHWAQETAPMRENAIYCMGMGHPLIRQMFHEISARLIRGGATSHIDD
                     IYWLTKLELEKLIVQLDKNIPLSDRRNSILARKAELKKYQGYMPPAQLPEKKVKSISH
                     APQKQKDGKTVLRGIGTSSGVVTARACVLNSPADFKNFQPGSVLVAVTTTPAWTPLFA
                     SASGIVTDIGGPLSHSSIVARECGIPAVMATHTATRSIKNGQMITVDGSEGTVTIDE"
     gene            12379..13539
                     /locus_tag="KRH54_001701"
     CDS             12379..13539
                     /locus_tag="KRH54_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8579367.1"
                     /translation="MNKKPIFALNLATLVIALGYSLILPVMPFYMENLGAGGRELGWL
                     TAVYALAQTVCAPFWGALSDRIGRKPIISIGIIGYSISLFLFGLASSFWTLFIARSLS
                     GILSSATSVASLAYVGDSSSEEERSKNMSQLGASMSVGVMMGPLLGGILAGYSLALPF
                     FTGAGISFIAFLLILTLLPESIERSGQTEKRKPFDFSDLKSIIFGSAGMVLILIFVVH
                     FCQTGLQGITGLYVVDKFGFSTKQVGVIWMVLAGILIVVQGFLTGPLAKKIGEKRLIL
                     IGMFCKALVAFAMVLTTGFVSVLVVFGLFAVSSAITVPTLNATLSKTDNQHKGTLMGF
                     ASTAGNLSKVIAPLLTGYLYESNIELPYITTGGIALIGVVICYIWIKNQKNK"
     gene            complement(13726..13919)
                     /locus_tag="KRH54_001702"
                     /pseudo
     CDS             complement(13726..13919)
                     /locus_tag="KRH54_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            14022..14528
                     /locus_tag="KRH54_001703"
     CDS             14022..14528
                     /locus_tag="KRH54_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG8579368.1"
                     /translation="MKNEKKITVALLIVYLLILSWIILFKLQFSFSVLDHIRQINLIP
                     FGGSVIANGKIDFDEIINNAIVFIPVGAYFSLLFKNKSVLKAIGSVLGISFVYEIIQF
                     IFAIGASDITDLISNTLGGIIGIALVYVLSIVLKDKTHKILNRIAIVCTVLVVAFLFM
                     LLAVNNIF"
     gene            14533..14808
                     /locus_tag="KRH54_001704"
     CDS             14533..14808
                     /locus_tag="KRH54_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HBG8579369.1"
                     /translation="MLITCCFGKDFNLSKFAFEIEHEITPMEQMKAINFSLEKDDCTS
                     KVLIYNPDKWKYVEYYFYKDRPIKKLLEKFIQFCICHNKICFEILNT"
     gene            15078..15650
                     /locus_tag="KRH54_001705"
     CDS             15078..15650
                     /locus_tag="KRH54_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG8579370.1"
                     /translation="MNVDTIRTDIPTSSAVTDVIVWNIEQSGTDTLSATYEVDQQIKE
                     REQTTAVKATYTVKVHVDADGDMVIVQNLTLAPAIEKSDYEPKTSETDASVDADTVND
                     ATAFLETFFEVYPTATEKELAYYVSENVIEPIGRDYLYSELVNPVFTKDGDNVKVKVA
                     VKFLDNQTKATQVSQYDLVLHKDSNWKILG"
     gene            15753..16568
                     /locus_tag="KRH54_001706"
     CDS             15753..16568
                     /locus_tag="KRH54_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBG8579371.1"
                     /translation="MFKIGEFSKLTQVSTRMLRYYDETGLLKPAKIDSLTGYRMYSAE
                     QISTLNRIIYLRDSGFNVAEIALALNFSNNAIIEQLDRKSIEIMDNIQKEQEKLKKIE
                     IAKKEVLHGKSELYFNITIKEIPSYCVLSLRKEIPNYYYEGELWKELSVFAKENKINI
                     SDETFSIYHNEEYKEKDVDVELCAIVNKIGKATGDFKFRFTEPVSMMACTMVYGDFFN
                     ISYAYRSFAKWLQENSQYKMKGSSRQIVHRGPWNEKNPENYLTEIQIPLENLL"
     gene            16649..17125
                     /locus_tag="KRH54_001707"
     CDS             16649..17125
                     /locus_tag="KRH54_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBG8579372.1"
                     /translation="MITYKVNSIGTIYNNEKGAFIQLEAAYIPALRALDGFSHINVLW
                     WFSDFDNTDFRSVLETEQPYKNAPEKMGIFATRSPVRPNPIALSVVEIINIDYMNGMI
                     QIAYIDANDKTPILDIKPYTPSLDRVENPNVPDWCSHWPHSLEESASFAWENEFNF"
     gene            17139..17690
                     /locus_tag="KRH54_001708"
     CDS             17139..17690
                     /locus_tag="KRH54_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8579373.1"
                     /translation="MSLQDYIIEKPVIDTEHLILRTMDKNDIDDLKEWISDISLYEYW
                     GKRPSKSEKNPEVLFSKPEKATKSFHWGIVHKQDSKVIGEIWIYLIENNRMAKVAFRL
                     SKQYQGNGLMSEALKSVIEFCFSKTELQKIWTDVHILNHASYKTLEKVGFQREGHIRG
                     GKMVNTYCDYYLYGILKSDYITS"
BASE COUNT         5860 a         3187 c         3972 g         5069 t
ORIGIN      
        1 acttggtttt taagcaacgc acagagttta gtgcttatgg cgattgtggt aattggtatc
       61 tacttaggtt ttaagcgtga gttttcaaaa cttatcggct tcttggtagt tgctcttgtt
      121 gccgttggct tggtattcaa cgctggtggt gtgaaagacg tattgttaga actgttcaat
      181 aagattatcg gtgcataaaa ttttattgta gtgtgagtgt agaaatggta tgatatggat
      241 atgaagaaag caacaaatta gaagttggag gaacaataat ggactacaaa gtagatgata
      301 aaaaccttga tgcatcaatg tttatttctt ttgtcaatca agtatggcag ggcgattatg
      361 atatggaaca aacaaggcaa gcattatcta aaacactaaa cataacagcc tatgacaata
      421 aaaagttagt agggtgtttg cgtattcttt ccgatggtta ctattttgga acaattacag
      481 agctacttgt tcttcctcaa tatcaaaaac agggtattgg aagtaaactt cttcaacttg
      541 caaaagacaa tacacctaca atgttatatt tcggttctca accagaagca gagaagtttt
      601 atgaaaagaa cggctgtcaa aaaagcttac aatcttacat gataaataag ggataataag
      661 agataacttc cagcttgaca attttatatc catatacaca aagcagttag tcttaggatt
      721 gactgctttt ttcttaccta aaaattagat tggagtgatg taatgaacga tatttttaag
      781 aatatgcagg caaaaatcgg ctgtgaatat ctttctgacc tgccctctta caaacgtaag
      841 gtgtggcatg aaatgaaaca actgaacctt gccgattatg aagagaaaca gttagaagat
      901 ttttcaaagt atgtgtttgg tatgtcatac cagaccttaa aagatgtgat gaaacaacag
      961 ataggacgtg aggaacaatg caggaaacaa gggtactggt ggaaacgaaa agaacaactg
     1021 acgaagaaac aacatcatac tggttcgacc tgccgataga ggttgccgaa tttgaggaaa
     1081 aattaggtgt cggtgcagaa agtggggaat accgtattat cgaaaaggta ttgccttatg
     1141 ctgatgaagt ccacgaacat acaagcgtgt accagctcaa caaattagat tttatgtacc
     1201 gccagctttc aagcgatatg caggaagaat atgtatcact acttatggtg tttgagaatt
     1261 tagaagcact ttatatttgc agaaatgtaa ttaccgttta tcccgactgt aaaagtatga
     1321 tagatgttgc aaggcaaaag ctgatgaacg acccgacgtt caaacattta tctgaggact
     1381 gtcaagaata ctatttcaat tttgaagcct acgcttctca cttgcaggaa cacgggaaat
     1441 ttttagtaac agaacacggt atctttgaac tgccagagta ggaagtgagg tggggcttat
     1501 gattgatgat atggcggttt atattgctaa tcttggcaaa tacaatgagg gatatttagt
     1561 cggtgcgtgg ttctctttcc ctattgacga ggaagatgtg aaagaaaaaa tcggtttgaa
     1621 tgaacaatat gaggaatacg ctgtccatga tactgacaac ttccctatcg acatagggga
     1681 atatgtttcc attgaagaac tcaatgagat gtatgaaatg atagaggaac ttcccgacta
     1741 tatcgtagag tgtctggacg aatttatcag ccactacggg acgctggaag aagtcgtgga
     1801 acacaaggac gatatttatt attatccaga ctgtgaaacc atgacagacg ttgcctacta
     1861 ctacatagac gaattgcagg cacttggcga cattccaccc agcttacaga actacattga
     1921 ctatgaagcc tacggtcgag atttggatat gggcggttgc tttattgaaa caagccgagg
     1981 tatgtgcgag ataccttatt aacgctggaa gatcagcgac aagcattgtt gctactgaca
     2041 gcgtatttct cttttaaggg acagtctgaa acatggctgt ccttttctct ttttaagaac
     2101 ttacgaaagg agctgaaaga acttgaaaaa gattaaaagc tacacgggta tctggaacgt
     2161 ggaaaaagtc ttgtatgcaa tcaatgactt taacttaccc tttcccgtta cctttacaca
     2221 gattacatgg tttgtaatta cagaatttat catcattctg tttggggata tgcccccact
     2281 ttccatgatt gagggagcat ttctcaaata ctttggtatt cccgttgctc tcacttggtt
     2341 tatgtcgcag aaaacctttg acggaaagaa gccgtacagc tttttgaaat cacagataac
     2401 ctatgccctg cgacctaaaa tcacttatgc aggaaaagcc gtaaaactgc ataagcagac
     2461 cttgaatgaa acaatcacgg cagtaaggag tgtgaactat gttcccgata aaatatattg
     2521 acaataacct tgtctggaac aaggacaatg aggtgttcgc ctactatgag ctgataccat
     2581 acaattattc tttcctatcc gcagagcaga aatttatcgt gcatgacagc tttcgacagc
     2641 taatcgcaca gtcccgtgag ggtaaaattc atgcgttgca gattgccaca gaaagctcaa
     2701 tacgaagtat gcaggagcag tcaaagaaac tggtaacggg gaaacttcgt gaggtggcag
     2761 tccaaaaaat agacgaacag accgaagcgt tagtatctat gattggggac aatcaagtgg
     2821 actaccgctt ttttcttggc tttaagctca tggttacgga agaacaactc aatctgaaga
     2881 atattaaaaa atcggcatgg ctgacgttca cggaatttct ccatgaagtg aaccacacgc
     2941 tgatgaatga ttttgtttcc atgccgaatg atgaaatcaa ccgttacatg aaaatggaaa
     3001 agttactgga aaataaaatc tcccgtcgct ttaaggtgcg tcgcttggaa atcaatgatt
     3061 ttgggtatct catggaacat ctttacggca gggacggtat cgcctatgaa gattatgagt
     3121 accagctacc agagaagaaa ttgcagaaag aaacgctgat aaaatactac gaccttatcc
     3181 gcccgacaag gtgtgtgatt gaggaaagcc agcggtattt acgattggaa catgaggaca
     3241 aggaaagcta tgtgtcttac tttactgtca atgcgattgt aggcgagctt gattttccgt
     3301 cgtctgaaat cttctatttc cagcaacagc agtttacatt ccccgttgat acttctatga
     3361 atgtagaaat cgtggagaac agaaaggctc ttagcacagt aagaaataag aaaaaggaat
     3421 tgaaagacct tgacaatcac gcttatcaag caggaagtga aaccagctca aatgtggtag
     3481 acgctttaga cagcgtggac gagctggaaa cagacttaga ccagagcaaa gaaagtatgt
     3541 ataagttaag ctacgtgata cgggtgtctg cacccgatct tgacgagctg aaacgccgtt
     3601 gtgatgaagt caaggacttt tacgacgacc tcaacgtaaa gctggtgcgt cctgctgggg
     3661 atatgctggg gcttcattct gaatttttac ctgccagcaa gcgatatatc aatgactatg
     3721 tgcagtatgt aaaatcagac ttcttggcag ggcttgggtt tggtgctacc cagcagttag
     3781 gggaaaatac gggtatctat atgggctatt ccgttgatac gggaagaaat gtgtacctgc
     3841 aaccgtccct tgccagtcaa ggtgtaaaag gtacagttac caacgccctt gcgtctgctt
     3901 ttgtcggttc gcttggcggt ggaaagtcgt tctgcaacaa tcttcttgtg tattattcgg
     3961 ttctctttgg gggacaagcg gttatcttag acccaaaatc agagcgtggc aactggaaag
     4021 aaacacttcc agaaatcgca catgaaatca atatcgtaaa tcttaccagc gacaaggaca
     4081 atgcaggact tcttgaccca ttcgtgatta tgaagaatgt aaaagacgct gaaagtctgg
     4141 caatcgatat cttaacattc cttactggta tttcctctag ggacggcgaa aaattccccg
     4201 tacttcgtaa agcggtacgc tccgttaccc agagcgacag tcggggcttg ctccatgtga
     4261 tagacgagct acgccgtgaa gatacgccca tatcaagaaa tatcgcagac catatcgaca
     4321 gcttcacgga ctacgacttt gcacacctgc tgttttcaga cggtacggta gaaaatgcta
     4381 tcagtctgga taaccagctc aatatcattc aagtagccga ccttgtgttg ccagataagg
     4441 acacgacctt tgaggaatac acgaccattg aattgttgtc tgtgtctatg ctgattgtga
     4501 ttagtacctt tgcccttgat tttatccatt cggacagaag catttttaag attgtagatt
     4561 tggacgaagc atgggcgttc ttaaatgtgg cacaaggcga aaccttatca aataagctgg
     4621 ttcgtgctgg acgagctatg caggcaggcg tctattttgt tacccagtct gcctatgatg
     4681 tttcaaagga aagtctgaaa aataatatcg gcttgaaatt tgcctttaga agtaccgata
     4741 taaacgagat taaacagacc ttagaatttt tcggtatcga caaagaggac gaaaacaacc
     4801 agaaaaggct tcgtgatttg gagaacggac aatgcttgtt gcaggactta tacgggcgtg
     4861 tcggcgtagt acagatacac ccagtctttg aagaactgct acacgccttt gataccagac
     4921 cgcccgtaca gagaaatgag gtggagtgat gaaagaaagg atacagagtg cgttcacaaa
     4981 aaagaagatg ttccgctttc tcaagataac ggtgtttgtc gtggcacttt cccttatcct
     5041 gctttcacta cttgaaacgg tggctcatgc aacggggctt gtggacgata cgataaatac
     5101 agaaaattta tattctaaat atccgttatc caactatcag cttgattttt atgtggataa
     5161 tagctgggct tggttgccgt ggaactggct ggacggtatc ggaaaatcgg tacagtacgg
     5221 gctttactgt attactaact tcgtttggac tatcagttta tatttaagca atgccacggg
     5281 ctatgtggtg caggaagcct ataagctgga cttcatcaat gatatggcag acagtatcgg
     5341 acagagcata cagacccttg caggcgttac ctcaagcggt ttttcaacct ctggcttcta
     5401 tgtcggtttc ctgcttctca ttatcttggt ggtgggaatt tatgtagcat atacgggact
     5461 gattaaaaga gaaaccagca aggcacttca cgccgttatc aactttgttg tggtgtttat
     5521 tttgtcggct tcatttattg cctatgctcc cgactacatc aaaaagataa atgaattttc
     5581 aagcgacatc agtaccgctt ctttggactt gggaacaaaa atcatgctcc caaactctga
     5641 cagcgagggc aaggacagcg tggacttgat aagggacagc ctattctcta ttcaagtgga
     5701 acagccgtgg ctactcttac aatttgggaa cagcaacgca gaggaaatcg ggacagaccg
     5761 tgtggaagct ctggtatcgg taagcccaga ggacgaggac ggaaaaacca gagaggaaat
     5821 cgtgaaaaca gaaatcgagg ataacgacaa caacattctg acgataccgc aggtagtaaa
     5881 ccgtttaggt atggtgtttt tcctcttatt cttcaactta ggtatcacga tatttgtatt
     5941 cttgcttacg ggcatgatgt tgttcagcca gatacttttt attatctttg caatgttcct
     6001 gcctattagt ttcctgctct ctatgatacc gagctatgaa agcatggcaa agcaggcaat
     6061 cgtgagggta tttaatacca taatgacacg ggcaggaata acgctcattg taacggtggc
     6121 gttcagcatt tccagtatgt tttacaatat ctccacagac tacccgttct ttatggtggc
     6181 gttcttgcag atagtttgtt ttgcaggtat ctatatgaag ctgggcgatt taatgagtat
     6241 gttctcactt aatgccagcg acagtcaaag catgggacgc aggatattcc gcagaccgta
     6301 tctgtttata cgacatagag cgagaagaat ggagcgacgt atggcaaggg cggtaagtgc
     6361 tggcggtatt tcgggcggtg tcgctggtgc gctggctggc agtaccgttt ctaatgcaag
     6421 gacaacaaga aacaatccta caaaggaaaa tcggggcaat accacttcca tcatgggaca
     6481 gcgtgcaggc tcaaagatag gcgctgtctt agatacgaaa aataaagtga aagacaaggc
     6541 aaatgctgtc aaagagaatg ttaaggatat gccgacacag accgcttatg cggtgtattc
     6601 tgcaaaagaa aaggcaaagt ccagcgtgtc cgacttcaag cgtggcatgg tgcaggaaca
     6661 gcaatccaga cagacgggac gcttagaaaa gcaggaacaa cgtaaaaaaa atatcgctaa
     6721 caagcgtatg gagcttcaaa aggcacaaga agcaaggcag gcacgaaagg ctgacggatc
     6781 agcgacaacg ggagctaccc gtccccatga gcgaccagcc acagcttcaa agccgagtgt
     6841 ggaaaaagtg caggaagtca aacgccctgc cacaactccg acttcaaaag caagtgagcc
     6901 agtcaagaca aatgttatca aagagcgtcc gctatcttcg ggtgcttctg acggaaaagc
     6961 aacccagtcg gcacagccaa tacataggca gaatatagaa aaagtggtat cgcaggaaac
     7021 acgccagaat tacgttaaag accgcaggac aaaggttcag cagacgcaga gcgtccagaa
     7081 gaaccagcag accacagaga aaaaccataa ccttgtaacg aagaaaggac agaagaaaaa
     7141 atgaaactga aacatatcgc tcttatcggc agtctgtttc ctatcctctt ttccatagtc
     7201 cttttttttg gggtgctgat tagtgcggac agcgacgacg aaaacagcaa cttttcttct
     7261 ggtattacgg gtttgaacct atccgcagaa gtcttgaaac atcagcctat ggtggaaaag
     7321 tacgccagag aatacggcat ttccgagtat gttaatgtat tactggctat cattcaagta
     7381 gaaagtggcg gtacagcgga agatgtcatg cagtccagcg aaagtctggg actaccgccc
     7441 aactctttag atacggaaag ctcaatcaag caggggtgta aatattttgc gtccctgctt
     7501 tcttcctaca aaaatcaagg aataaatgac ttgaatgtgg cgatacagtc ctataactat
     7561 ggcggtggct atgtgggata tgtggcagga aaagggaaaa agcatacgtt tactcttgca
     7621 gagagcttcg cctgtgaaaa atcgggtgga aagaaagtaa cctacaccaa cacgatagcc
     7681 gttgcgaaga atgggggctg gcggtatggc tatggaaata tgttttatgt ggaattggtc
     7741 aatcaatatc tgactgtacc gcaggtatcg ggagaactgg cacaaaagat aatgaatgaa
     7801 gcgttgaagt atcaaggttg gaagtatgtc tatggtggca gtaatccgaa cacttccttt
     7861 gactgttcgg gacttgtgca atggtgttat ggaaaagcgg gtatctcttt accgagaacg
     7921 gcacaagccc agtatgacgc aacccagcat atccccttgt cgcaggcaaa ggctggcgat
     7981 ttggtgtttt tccattcaac ctataacgct ggttcgtatg taacccatgt cggtatctat
     8041 gtcgggaata atcagatgta ccatgcaggc gacccgatag gatatgcaga cctcaatagt
     8101 agctactggc aacagcactt aatcggtgca ggacgagtaa aataaaagaa aggacttaga
     8161 aaatatgttt aagaaaagta agaaacagac agaaaatgtc aaagaaaaaa aggtgcgtac
     8221 tgtcaaggta ggcacacata agaaaactgt aattgcgttt tgggtggtgc ttatcgtaag
     8281 tgtaagtttt ggggtatata agaattttac cgccattgat atgcacaccg tacatgaaac
     8341 agaaaccata caattaagac ttaccgatac taacgggatt gaaaatttcg tgaagaactt
     8401 tgcgaagtcc tattacacat ggaacaacag caaagaagcg attgaagcaa ggacgcaggc
     8461 aatcagcggt tatctgacaa aggaattgca ggacttgaat gttgatacca ttagaaccga
     8521 tataccgacc agttccacgg ttacagatgt gattgtttgg aatatcgaac agtcaggaac
     8581 ggacactttt tctgctacct acgaagtaga tcagcagata aaagagggag aacagacaac
     8641 agcagtcaag gcaatctata cggtaaaagt ccatgtggac gttgacgggg atatggtaat
     8701 catacagaac cctacgcttg caccaacaat cgaaaaatcg gactatgagc taaagacacc
     8761 agaagcagac gcaagtgtgg acgctgatac cataaatgac gctacttcat ttttggaaac
     8821 attctttaag ctgtacccaa tagcgacgga aaaagagctt gcctattatg tatcgggaaa
     8881 tgtgcttgaa cctatcggca aggactatct ttattctgaa ttgataaacc cagtctttac
     8941 aaaagacggg gacaatgtga aagtcaaagt tgccgtgaaa ttccttgata atcaaacaaa
     9001 ggcgacgcag gtatcgcagt atgaacttgt gctacataag gatagtaact ggaaaattgt
     9061 gaaataataa tttaaagcgt gcatttctta taagatttgt acgctttact tttatggtaa
     9121 aaattagcct tgacaagcga ccgttggtcg ctatataatg aaagcgaaac aagaaacgac
     9181 cgatggtcgc tataaggagg tatcataaat atgccaaaag gaattatgct tacacaagaa
     9241 caacaagaag aacgccgaga agaaataatc agtgttgctt tgcagctcat agagaaaaat
     9301 ggattccaaa aaacatcaat gaggaaaatt gcgattttag caaacatggg aaagtccagt
     9361 ctgtatgatt ttttcaaaac gaaagacgag atttttgtat atgcagttga gaaagaaata
     9421 gaagaaacaa ttaaaaaggt tcataggatt attgccgatg aatcctcgcc ggaacagtgt
     9481 cttagaaaaa tcatgctgaa tcatcttgga gtaccaaagc agtatcgaac ggtgctgatg
     9541 tggttaaata cagaatctga ttatttagaa gaagattatc gaaagcggct gaaaactgcg
     9601 cgttacgcat atcaggatat tataaaatct gtaattgaaa atggaatgga atcaggcgtc
     9661 ttccgtaaga cgaatgccaa tcttatgacg cgtttgttaa ttaactccat tatatcaatc
     9721 atttacacat cccgaccatc agccagtccg gagaaaatgc ttgatgaaac aatggatata
     9781 tttctacatg gaattatgaa tgatggaggt gaattattat gatttattat actctaccct
     9841 tgttgcataa acaggcaacc cttgaaatgg tcggcggaaa aggtatgtct ttatcaaaac
     9901 ttttgacaac gggtattcct gtgccggagg gctttcatgt tacgactact tcatacaaga
     9961 tttttgttga aaagaaccat attcagcctt acattaataa gttgctggac ggtattgaca
    10021 ctaacaatac gagccagctt gaaaatgtat ctacgcagat agggatgctt ttccataatg
    10081 gagaaatgcc gcaggaagta tcagatgcaa ttaaaaccgc atatgccaga ttgggaaata
    10141 tagcggtggc tgtccgttcc tccgcaaccg ccgaggattt gcccgatgct tcttttgcag
    10201 gccagcagga aacctatctt aacatacaag gtgagaatga agttcttgac gctgtaaaga
    10261 ggtgctgggc ttccctatgg acagctcgcg ctattgctta ccgcgtgaag aatgatataa
    10321 agcaggaaat cgtcgcactt gctgttgtag tacaaaagct tgcgttttcc gatgcgtccg
    10381 gcgttatgtt tacgctaaac cctatcaatg gcagacgtag cgaaatgatt gttaacgccg
    10441 cgtggggact tggcgaatcg gtggtttcta gcttagtcac ccctgataca atcgttgtag
    10501 ataaaaatgc tgaacgaatt gtatcgtatg aagtagcaaa caaagagatt atgacagtac
    10561 gcaactcaga tggaacagaa gaaatcccaa ctcctgaaca gttgaggaaa aaacatgctc
    10621 ttactcgcaa tcaagttatg cgattgacac agcttggaaa aaaaattgag aagtattatg
    10681 aaatgcctat ggatgtagag tgggcactgg aaaaagataa gttgtatatt gttcaggctc
    10741 gccccattac tgtcctaccg ccggaatgga cgttaccaga aaaggatgtt atatatacaa
    10801 gaggcagtct agcggaacac ttgccaaatc ctgttacgcc cttattcgcc acacttgggc
    10861 ttgaaatagt caaccgtgcg tcggcgcttt tatggataga tatgtttggt aaaagtgcga
    10921 aaaagctact gccagaaaac ggagcatata cgattattaa cggatatgtt tatctttccg
    10981 ctaattcaaa gcctcttttg attgctgtca aatccctttc accccgatct ttacgccgtg
    11041 cgctcacgaa cagtgttgca cgctgggaaa cagcacgaaa agaatttgag gatgtgatta
    11101 aacaatggga agaaaagccc atgcatatgc tgaatgctca tcaaataatg gaggggattc
    11161 agactgtatt ttatggagcg tgtatttatt tcacgaggat tcagcttaca ttgccagccg
    11221 cctctatcag cgaaacatta tttacaaaat tttttcaggg ggcggctcgt cgcgctggta
    11281 tgacagatac ttctgttttc ctgcttggat ttgatacgat tgctctgcaa gccgaaaaga
    11341 acctgtggag tctctcggaa tgggtaaagc aaaataatac tctcaacctt tatctgcaaa
    11401 gtaatccaac agcaaagata gcggaaaatt ttatgtcctc agttccgcct gacgaagttt
    11461 cactggaagt atggatagaa tggaaaaatc gaatcaatca gtattttaaa gagtttggtc
    11521 gtaccgctta tgaatttgat tttgcatatt ccacaccgca ggaaacactt acaccaacct
    11581 ttgaatcgat aaaaaccttt gtagaaggca aaggtgaaag tcctttttta cgtcaatcca
    11641 aatttgaaaa gcataggaaa caggcagaag aagaaatttt acaacatata ggtagctcac
    11701 gcaaaaaact gtttttgaag ttgctccatt gggcacagga aactgctcct atgcgtgaaa
    11761 atgctattta ttgcatggga atggggcatc ctctgattcg ccagatgttt catgaaattt
    11821 cagcacgcct tatacgtggc ggagctactt cgcatataga tgatatttat tggctcacaa
    11881 aattggaatt ggaaaaactc atagtacagt tagacaaaaa tataccttta tccgatagaa
    11941 gaaattcgat acttgcgcga aaagcggaac tcaaaaagta tcagggctat atgccaccag
    12001 ctcagttacc tgagaagaaa gtaaaaagca tatcacatgc accacagaaa caaaaagatg
    12061 gaaaaaccgt gttgaggggt ataggaacca gttctggtgt cgtgacagct cgtgcctgcg
    12121 tactaaatag tccggcagat tttaaaaatt tccagccagg aagtgttttg gttgccgtta
    12181 ctacaactcc ggcttggaca cccttatttg cttcagcaag tggaattgta acggatattg
    12241 gaggtcctct cagtcattca agtattgttg cacgggaatg tggtatccct gccgttatgg
    12301 cgacacacac agccacgaga agtattaaaa atggacaaat gataacggtg gacggctcgg
    12361 aggggacggt gacgattgat gaataaaaag ccgattttcg ctttaaatct tgctactttg
    12421 gtgattgcgt taggatatag cttaattctt ccggtcatgc cattttacat ggaaaatcta
    12481 ggtgctggcg gtcgtgagct tggatggctc actgccgtat atgccctggc acaaacagta
    12541 tgcgcacctt tttggggtgc actgtccgac cgaattggta gaaagccgat tatcagcatt
    12601 ggtattatag gatattccat cagtttgttc ctgttcggtt tggcttcatc cttttggaca
    12661 ctatttatag ctcgcagttt atcgggtatt ttgtcatctg ctacgtccgt tgcatctttg
    12721 gcatatgttg gagattccag ctcggaggaa gaaagaagca agaatatgag tcaacttgga
    12781 gcttcaatga gcgttggcgt aatgatggga ccattattgg gaggcattct tgcaggctat
    12841 tcgcttgcgc tcccattttt tacaggagct ggcatttcat ttattgcgtt tctgctaatt
    12901 ctaacacttt tgccagagtc aattgagcgt tcaggacaga cggaaaaaag aaaacccttt
    12961 gatttttcag acctgaaaag tataattttt ggctcagcag gaatggtttt gatacttatt
    13021 ttcgtcgtgc atttctgtca aacaggcttg caaggaatta cggggctgta tgtcgtagat
    13081 aagtttggat tttccactaa gcaagttggt gttatttgga tggtgctagc aggtattttg
    13141 attgtagtac aaggcttttt gactggtcct ttagcaaaaa aaattggtga gaagcgacta
    13201 atactgatag gtatgttttg caaagcctta gtagcgtttg ccatggtatt gacaactggg
    13261 tttgttagtg tgttggtggt ttttggctta tttgcggtat cttctgctat tacagtaccc
    13321 acattaaacg caaccctttc aaaaactgac aatcagcata aaggaacact gatgggattt
    13381 gccagtaccg ccggaaatct tagcaaagtt attgctcctt tattaactgg ttatctgtat
    13441 gaaagcaata ttgaattacc atatataacc acgggaggga tagccttaat cggagtggtt
    13501 atctgctata tctggataaa aaatcaaaag aacaaatgat ggaagaggac gtttatatgg
    13561 aggttataca cagtaactaa tctggtatag aaattcaatt gaggcttttc tgctttatta
    13621 aagtcttgct actacttaaa cctataatat ccttaattca ttatgacaaa ttccaattca
    13681 acagttttga tacacataag gaggtaacaa tactatggaa aattatcaat gaagtgctaa
    13741 ttctttatca gtgaagttat ccataagttt ttctacctgt aatcgtctgg tgctttttgc
    13801 cagatcattg gtaggtaaga aaaattcatc aacagacaca tgaagtaatg atacaaggtc
    13861 acttgtatgc ttggatgttg ccctttattt tcaatattag ttaagtaacg tgggtcaata
    13921 tttgtaattc gattttaggg aaatatttaa agaaatattt tcgttttatt tctttaaatc
    13981 ttcaagaata ttgatagttt ttttgaaagt aggtgtgtat tatgaagaat gagaaaaaaa
    14041 ttacagtcgc attgctcatt gtttatttat tgattttatc atggattatt ttatttaagc
    14101 tacaattttc attttctgtg ttagaccaca taagacaaat aaacttaatt ccttttggtg
    14161 gttcagttat tgcaaatgga aaaattgact tcgatgaaat tataaacaat gccattgttt
    14221 ttatccctgt aggtgcatat ttcagtttgc tttttaaaaa taaatctgtt ctaaaagcaa
    14281 taggttctgt tttaggaatt agctttgtat atgagataat tcaatttata tttgctatcg
    14341 gagcaagtga tattacagac ttaattagca acacattagg gggtataatt ggaattgcat
    14401 tagtatatgt attatctatt gttctaaagg ataaaacaca taagatatta aatcgaatag
    14461 caatagtatg tactgtatta gtagttgcct tcttatttat gcttcttgct gtaaataata
    14521 tattttaatg ccatgttaat tacatgttgt tttggaaaag actttaatct gtcaaagttt
    14581 gcttttgaaa ttgaacatga aattacacca atggaacaaa tgaaagctat aaatttttca
    14641 ttggagaaag atgactgtac aagtaaagta ttaatataca atccagacaa atggaaatat
    14701 gtagaatact atttctataa agatagacct attaaaaaac tattggaaaa atttattcaa
    14761 ttttgcattt gtcacaataa aatatgtttt gaaatattga atacttaaat atgcaaaaaa
    14821 aacaaatgta actggctatg gggctatatc tactaaaaag ctatgtgata ggttgggatt
    14881 aaatttaaac atcacttcta gaaggcatga atacacaagg ctgacaaact atacagttat
    14941 gattaaacga taccaacggg attgaaaatt tcgtgaagaa ttttgagaag tcctattaca
    15001 catgagcaac agcaacgaag ctattgaagc aaggacgcag gcaatctagc ggttatctga
    15061 caaaggagtt gtaggacttg aatgtagata ctatcagaac ggatataccg accagctctg
    15121 cggttacaga tgtgattgtg tggaatatcg agcagtcggg aacggacact ttatctgcta
    15181 cctacgaagt agatcagcag ataaaagaga gagaacagac aacagcggtc aaggcaacct
    15241 ataccgtaaa agtccatgtg gacgctgacg gggatatggt aatcgttcag aaccttactc
    15301 ttgcaccagc aatcgaaaaa tcagactatg agccaaagac atcagaaaca gacgcaagcg
    15361 tggacgctga taccgttaat gacgctaccg catttctgga aacattcttt gaggtgtacc
    15421 cgacagccac agaaaaagaa cttgcctatt atgtatcgga aaatgtgatt gaacctatcg
    15481 gcagggacta cctttattct gaattggtaa acccagtctt tacaaaggac ggggacaatg
    15541 tgaaagtcaa ggtagctgtg aaattccttg ataatcagac aaaggcaacg caggtgtcgc
    15601 agtatgatct tgtactccat aaggatagca actggaagat tttaggataa catggaaatg
    15661 ggcttgtatc ataattatag tgtgcaagcc cttttcatat tgaagtatca ataatcaata
    15721 attataatta ggtacatgaa tggagggata aaatgttcaa aattggtgag ttttcaaaat
    15781 tgacacaagt ttctactcgt atgttgcgtt attatgatga aacgggttta ttaaaacctg
    15841 ctaaaattga ttcattaact ggatatcgta tgtattcagc agaacaaatc tcaactttaa
    15901 atagaattat atatttacgt gatagtggct tcaatgtcgc tgaaattgca ttagccctca
    15961 atttcagtaa taatgccatt atagaacagc ttgatagaaa gtctattgaa attatggata
    16021 atatacaaaa agaacaagaa aaactaaaga aaattgagat tgctaaaaaa gaagtgttac
    16081 atggcaaaag tgaattgtat tttaacataa ccattaaaga gataccgagt tattgcgttt
    16141 tatctttaag aaaagaaata ccaaattatt attatgaggg cgaactctgg aaagaactct
    16201 ctgtttttgc taaagaaaat aaaataaata tttctgatga aacattctca atatatcaca
    16261 atgaagaata taaagaaaaa gatgttgatg tagaattatg tgccattgta aataaaatag
    16321 gaaaagctac aggtgatttt aagtttcgtt ttaccgaacc agtttctatg atggcttgta
    16381 ctatggtata tggagatttt tttaatattt cttatgctta tcgttcattt gctaaatggc
    16441 ttcaagaaaa cagtcaatat aagatgaaag gttcaagccg tcaaatagtt catcgtggac
    16501 cgtggaacga gaaaaatcca gagaactatc taacagaaat ccaaattcct ttagaaaatc
    16561 tcttgtaaaa aatgataatc tgccttgact ttaacatggt gttaaggttt atcgtttatt
    16621 tatcaagaag aaaaaggagg aaaatataat gataacttat aaagtgaatt caataggtac
    16681 tatctataat aatgaaaaag gtgctttcat tcaacttgaa gctgcatata tcccagcatt
    16741 aagggcatta gacggattca gtcatataaa tgttttatgg tggttcagtg attttgataa
    16801 tacggatttt agaagtgtat tggaaacaga acagccttat aaaaatgctc cagagaaaat
    16861 ggggattttt gcaacaaggt cgccagtacg tccaaatccg attgctttat cagtagtgga
    16921 aattatcaat atcgattata tgaatggaat gatacaaatt gcttacattg atgctaatga
    16981 caaaacgcct atcttggata ttaaaccata cacaccgagt ttggataggg tggaaaatcc
    17041 aaatgttcca gattggtgta gtcattggcc tcatagttta gaagaatccg cttcttttgc
    17101 atgggaaaac gaatttaatt tttaaggagt gatgaaccat gagtttgcag gattacatta
    17161 ttgaaaaacc agtaattgat acagaacacc ttattttaag aacaatggat aaaaatgaca
    17221 tagatgattt gaaagaatgg attagtgata tttctttata cgagtattgg gggaaacgtc
    17281 cttcaaaaag cgaaaaaaat ccagaagtac tctttagtaa gccagaaaaa gctactaaaa
    17341 gttttcattg gggaattgtc cataaacaag atagcaaagt gattggtgaa atatggatat
    17401 accttattga aaataataga atggcaaaag tggctttcag actttctaag caatatcaag
    17461 gtaatggact aatgagtgaa gcgttgaaaa gcgtaattga attttgtttt tcaaaaacag
    17521 aattacaaaa aatatggaca gatgtgcata ttttaaatca tgcttcttac aagacattgg
    17581 agaaagtcgg ttttcaaagg gaaggacata ttcgaggggg aaaaatggta aatacctatt
    17641 gcgactacta cctatatgga attttaaaat ctgattatat cactagctga tgttatggct
    17701 aatattgaag cgacgattgg cgaagcaatg aacaatacag ttccaaaggt ctatgatacg
    17761 aaaagcatta aaagatatgt gatgaattta ttagatatgg ctcattaaaa agtaccaaag
    17821 aaataaaagt taccatatag acaaaattgg ttttttatat cttttgattt cttataagat
    17881 aatggcattg tgcgacaagc tatacaaaaa attttcatgt atactttgat tataaattat
    17941 acaaggagct aactatatat aaatcaagtg atttttgata attagctgaa atatttttta
    18001 gatcattgaa aagtgaattt tgtgtacact gaaaactact gctttatgca gtactttaaa
    18061 aatattcaat tgaatgcttt taaataag
//
LOCUS       DAEOSP010000060        17834 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.060, whole genome shotgun sequence.
ACCESSION   DAEOSP010000060 DAEOSP010000000
VERSION     DAEOSP010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17834
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.060"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            240..1844
                     /gene="rho"
                     /locus_tag="KRH54_001709"
     CDS             240..1844
                     /gene="rho"
                     /locus_tag="KRH54_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBG8579374.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            1997..2197
                     /gene="rpmE"
                     /locus_tag="KRH54_001710"
     CDS             1997..2197
                     /gene="rpmE"
                     /locus_tag="KRH54_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBG8579375.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            2342..3235
                     /locus_tag="KRH54_001711"
     CDS             2342..3235
                     /locus_tag="KRH54_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBG8579376.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            3253..4101
                     /gene="prmC"
                     /locus_tag="KRH54_001712"
     CDS             3253..4101
                     /gene="prmC"
                     /locus_tag="KRH54_001712"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBG8579377.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            4145..5209
                     /gene="prfA"
                     /locus_tag="KRH54_001713"
     CDS             4145..5209
                     /gene="prfA"
                     /locus_tag="KRH54_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBG8579378.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            5338..6018
                     /locus_tag="KRH54_001714"
     CDS             5338..6018
                     /locus_tag="KRH54_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBG8579379.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            6047..7087
                     /locus_tag="KRH54_001715"
     CDS             6047..7087
                     /locus_tag="KRH54_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBG8579380.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            7104..7553
                     /locus_tag="KRH54_001716"
     CDS             7104..7553
                     /locus_tag="KRH54_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBG8579381.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            7570..8022
                     /gene="rpiB"
                     /locus_tag="KRH54_001717"
     CDS             7570..8022
                     /gene="rpiB"
                     /locus_tag="KRH54_001717"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBG8579382.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            8058..8687
                     /gene="upp"
                     /locus_tag="KRH54_001718"
     CDS             8058..8687
                     /gene="upp"
                     /locus_tag="KRH54_001718"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBG8579383.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            8988..9803
                     /locus_tag="KRH54_001719"
     CDS             8988..9803
                     /locus_tag="KRH54_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBG8579384.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            10029..10466
                     /locus_tag="KRH54_001720"
     CDS             10029..10466
                     /locus_tag="KRH54_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBG8579385.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            10651..10878
                     /locus_tag="KRH54_001721"
     CDS             10651..10878
                     /locus_tag="KRH54_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBG8579386.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            10883..11269
                     /locus_tag="KRH54_001722"
     CDS             10883..11269
                     /locus_tag="KRH54_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBG8579387.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            11272..11976
                     /gene="atpB"
                     /locus_tag="KRH54_001723"
     CDS             11272..11976
                     /gene="atpB"
                     /locus_tag="KRH54_001723"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBG8579388.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            12045..12305
                     /gene="atpE"
                     /locus_tag="KRH54_001724"
     CDS             12045..12305
                     /gene="atpE"
                     /locus_tag="KRH54_001724"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBG8579389.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            12428..12925
                     /gene="atpF"
                     /locus_tag="KRH54_001725"
     CDS             12428..12925
                     /gene="atpF"
                     /locus_tag="KRH54_001725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBG8579390.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            12922..13467
                     /locus_tag="KRH54_001726"
     CDS             12922..13467
                     /locus_tag="KRH54_001726"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBG8579391.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            13484..14986
                     /locus_tag="KRH54_001727"
     CDS             13484..14986
                     /locus_tag="KRH54_001727"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBG8579392.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            15014..15892
                     /gene="atpG"
                     /locus_tag="KRH54_001728"
     CDS             15014..15892
                     /gene="atpG"
                     /locus_tag="KRH54_001728"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBG8579393.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            15905..17299
                     /gene="atpD"
                     /locus_tag="KRH54_001729"
     CDS             15905..17299
                     /gene="atpD"
                     /locus_tag="KRH54_001729"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBG8579394.1"
                     /translation="MANVGKVVQIVGAVLDVKFDGEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            17302..17562
                     /gene="atpC"
                     /locus_tag="KRH54_001730"
     CDS             17302..17562
                     /gene="atpC"
                     /locus_tag="KRH54_001730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBG8579395.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
BASE COUNT         7092 a         1988 c         3476 g         5278 t
ORIGIN      
        1 atagttgcat attttaaagg aaaatattat tataagatat aaatatagtg ataaatttat
       61 aaaaacgtgc taagattgga cttgaaatac agtaatatag taggtaataa tgttttaaat
      121 tttatttatt gaccataata tataaataat gatattatta gacttaactg tttaaataaa
      181 acttttcttt ttcatgcctt caatttccaa aataagtatg tatataaatg aatatataga
      241 tgaggaggat ttttttgatt ttagatgata aagtattaga aggattaggt agtaagacct
      301 tagttgagct acgagagata gctaaagaat taaaaataaa atcaattact acatataaga
      361 aaaatgagtt aattgagatt ataaattcca aaagtaaaaa tgaggaaaaa gtaaacgaag
      421 agactaaaga aaaaaatgaa gatattaaag aagacataaa gaatagaaaa gaaaatatag
      481 agcgtgaaag tatagaaaaa gaagttgaat ataagagccc taccaaatca tacaacaaga
      541 aagaaataga tacccaaaat atttcccaaa accaagaaca tagaaatcaa agaaatgact
      601 ataataagac aacgagtgta aatgatagca ataagtcatc aaatatatct aatagaatgg
      661 tcagaaataa taataaaaac tattatatgc ctaaacaagt tgatgaatct aagatagtag
      721 atgaatttaa cacatcaaaa gaggatgaag tagtaggtgt acttgaaatt ctgccagatg
      781 gatttggttt tttaagaggt tcaaattatt tgtctactga aggagatgtt tacgtttcac
      841 catctcaaat aagaagattt aatatgaaaa caggagacaa gattaaggga ataactagac
      901 atccaaaaag tggagagaaa tttagagcac ttttatatgt tcaaaaaata aatgatgaaa
      961 accctgacac agctatacaa agaaatgcgt ttgagacatt aacaccaata tttccagaag
     1021 aacgacttac actggaaact aatagaaatg aaatagctac aagaattata gatttaattt
     1081 cacctatagg aaaaggacaa agaggattga tagtagcacc tccaaaagct gggaaaacag
     1141 ttcttttaaa aagcgttgca aatagcatag ctaaaaatca tccaaatgtt gaacttatag
     1201 tactccttat agatgaaaga ccagaggaag ttactgatat gaaagaatct attgaaggag
     1261 acgttatata ttccactttt gaccaagtat caagtcatca tgtcaaggtt gcagagatgg
     1321 tgttaaatag agctcaaaga ttagttgaac atggtaaaga tgtagtaatt ttactagata
     1381 gtataactag attggcaagg gcatataacc ttacaatatc tcctacaggt agaactttat
     1441 ctggaggtat tgaccctggt gcacttcatg gacctaaaaa gttctttggt gcagcaagga
     1501 atattagaca gggtggttct ttgacaatat taggaacagc attggttgaa actggttcta
     1561 ggatggatga tgtaatattt gaggaattta aaggtacagg aaatatggag ctacatttag
     1621 atagaaaatt agcagaaaaa agaatattcc ctgcaataga tatttacaag tctggtacaa
     1681 gaagagatga tttattgctt gatgatgaag aaaagactgc tctttggaga ttaagaagag
     1741 aaatgagtaa taattcagta atggaaatta cagataaagt cattgaactt ataaagagaa
     1801 ctaaggacaa taaagagttt gtaaaatcaa taaagaattt gtaaaatcaa taatacaaat
     1861 gcaaatatgg attatataaa ctaatagaaa aatcacttga atggcataat aagttgtgct
     1921 ataataatgt agttgataat gtaaaaagaa agttaatata attaattttt ttaaataaag
     1981 agaagaggtg actataatgc aaaaggaaat acaaccaaaa tacaatccag ttgaagtgcg
     2041 ttgtgcttgt gggaacacat ttgttgctgg ttcaactaag gacgaaataa aagttgaaat
     2101 atgttcagag tgccatccat tctatacagg aaaacaaaag aacatagaaa aaggtggaag
     2161 aatcgacaaa ttcaagaaaa gattcaaaat ggactaattt ttataaaagt ggagttggag
     2221 gaagccaact cctattttat tacaaaaaaa cattcatatt ctactttaaa ttgtcttttt
     2281 gtattataat aataaatatg taaaattgag tattctaaat acaataaaat gaggtgagtg
     2341 gatggcaaaa caagctgtag gtgggcaagc tgttattgaa ggtgtaatga tgcagtcaaa
     2401 agacaaaaga gcggtagctg ttagaaaaag tgacggagaa atagcactaa aagaagatag
     2461 aataaaaagt tgggtaagag ataagaatat agataagata ccttttgtta gaggttcttt
     2521 tgttatgata gatacaatga ttcaaggcat aaaaagttta aacttttctt cagagttttt
     2581 tatggaagaa gctgaagagg ataaatttga tttatttata aagaaaattt ttaaagataa
     2641 ggcaaatgat ataattataa tattttcatt ggtcatagca atgttacttt ctgctggatt
     2701 attcattttt atacctacat tggtaggagg agctttttct aaagtaatgc ctaatgattt
     2761 tatgcttaat ttaatagaag gaatcataag gatagctata ttatttgcat atatagtatt
     2821 gatttcacga agtaaggata tagaaagagt ctttcaatat catggagcag aacataaatc
     2881 tatatattgc tatgaaaatg atttagaact tacagtagag aatgctagaa aatttaaaag
     2941 attacatcct agatgtggta caaatttcct atttatagtt atggctgtat caataatatt
     3001 gtttgcattt tttggttggc caaatccaat tcttagaata tttatgagaa ttatatgtgt
     3061 gcctatagta gcaggtttat cttatgaggt aattagagtt cttgggaaat atgataatgg
     3121 atttactaaa attattgcat atccaggaat gatgttgcaa tattttacaa caaaagagcc
     3181 tgatgatgag caattagagg ttgctttgga agcattgaaa gcagtagtag attagagaaa
     3241 aggatatttg ttatgacgat aaaagatata attattaaat attcagataa actaaaagat
     3301 ataagtgata ctccaagatt agatacagag ttattgttac aaaaaacatt gggtgttgat
     3361 agattatata ttcacttaaa tctgaataaa gagctaactg aagaacaaaa aacaaaattt
     3421 atgggttttg cagaagaaag attaaatgga agaccaatag catatatagt tgaaaatagg
     3481 gagtttatgg gattagattt ttttgttaag gaaggcgtat tgattccaag accagataca
     3541 gaaactttag tagaagaaat aattgaaata tgtagagaaa aaaaagatgt aagtatatta
     3601 gatataggaa ctggttctgg agctattaca ataagtttag caaagtacat tgaaaattct
     3661 aaaatcatgt cttttgatat atcagagaca gcattagaaa tagctaaaaa aaatgctatt
     3721 ataaatgaag ttggtgaaaa aataaagtat attaattctg atttatttac agcaataagt
     3781 gatagtaaca taaaattcga tatcatagtt tcaaatccac catatattaa aaaacaagat
     3841 atagaaactc tgcacaaaca agttaaagat tatgaaccat ataatgctct tgaaggtgga
     3901 gaagatggat tagactttta tagaagaata acagaacaag gtaagaaata tttaaataag
     3961 tgtggaatat tagcatatga agttggtcat aatcaagctg aagatgtaat aaatattatg
     4021 aaaagtaatg gatataaaaa aatatacaca aagaaggata ttcaaggcat tgatagagtt
     4081 gttataggtt acaatatatg atataatcaa caattgatat gttacattta atcgaggtga
     4141 aagtatgtta aaaaaattag aggtattgga agatacatat aaagacttaa gtgaaaaaat
     4201 aggtgatccg gatgtaataa atgatcaaaa agtttggcag aaatatataa aagaacatgc
     4261 tgacttggaa cctattgtta tgaaatacag agagtataaa agcgttttag atagtataaa
     4321 agaatcaaaa gaaattttac aagaagagtc agatgaagaa ttaagagaat tagctaaaat
     4381 ggagttagct gagatggaag aaaaagtagc acctctggag gaagaaataa aaatactatt
     4441 attgcctaaa gaccctaatg atgataagaa cgttatagtt gaaattagag gtggagctgg
     4501 aggagatgaa gcagctctat ttgctggtga tttatttaga atgtactcaa ggtatgctga
     4561 aagaagaaga tggaaaatag aattattgag tgcaagtgat actggagttg gtggatataa
     4621 agaagtatct tttatgataa aaggtaaagg tgcttattca agattaaagt atgaatctgg
     4681 agttcataga gttcaaagaa taccatcaac tgaatctggt ggaagaatac acacatcaac
     4741 ttctactgtt gcagtattac cagaagttga agatgtagag gttgaaataa atccaaatga
     4801 tttaagaata gatgttttcc gttcttcggg taatggagga caaagtgtca atactactga
     4861 ctctgctgtt agggtaacac atatacctac aggtgaagta gtatcttgtc aagatggaaa
     4921 atcacaatta aaaaataaag agcaagcttt aaaaatatta aaagctagac tttacgataa
     4981 agcattggca gaacaacaca aagatatagc agcagaaaga aaaagccaag tcggaacagg
     5041 agacaggtca gaaagaataa gaacatataa tttccctcaa ggtagaataa gtgaccatag
     5101 aataaattta actttatata agttagatgc attcttagat ggagatatag atgaaatgat
     5161 agatgcttta attactgttg accaaacaga aaaaatgact gcaatataag taatgtttta
     5221 aagaagctgt ttcttcctag aaattatttt ctattttgaa acagctattt tgcattttag
     5281 gtcatgttat catattctat tgtatataat tgttatatac aagtatagga gggttatatg
     5341 atactaaaag taacaataat tgggcttttg gcaggtgtta tagggacagg attaggagga
     5401 gtaatatcag ccatttttaa aagagaggtc gataaatatc taagtttttt tatgggatta
     5461 tctggtggaa taatgttggc tgtagtggtg tttgatttaa tgaaagaatc catggacaaa
     5521 atggggataa tcaatacagt tatatttacg tttgttggag ctctcataac tatgtacata
     5581 aaaacaaaat tagatgtgtc tggaaatatg gcatctggat atcttatatt cataagtata
     5641 ttactacata atttaccaga aggactagcc attggttctt cttttatgtc aactgaaagt
     5701 ttaggtatta cacttgctat agttataggt ctacataata taccagaagg gttagctatg
     5761 gcattaggct tagtttgcaa taaaatgaaa ctaagtaaag ttattttgtt tactgtaata
     5821 gctggattac ctatgggtct aggaagtttt ttaggtgttt attttggagg agtatttact
     5881 tctcttatag gagtatttct tgctacagca ggaggtacta tgatgtatgt tgtattggaa
     5941 gaaatttttc cacattcaaa aagtgtgtat tgtataattg gtttcttgtt aggtacaatg
     6001 attgtaaatt atatataaaa ttaaaatgaa agcaggcata caaaagatga aaactattat
     6061 aagtaatatt gatataaata atattgatta tgaggaaata aaaatacaag caaaacttct
     6121 tagagaaggt aaaacagtta tttttccaac tgagacagta tatgggttgg gtgcaaatgc
     6181 attagatgaa aatgcagtaa agaagattta tgaagctaaa ggaagaccta gtgacaatcc
     6241 attgatagtc catatatatg aaaaagaaga agtatatgac ttagccaaag atataagtga
     6301 taaagctaaa cttgttatag aaaagctttg gccaggacct ataacaatta tattaaataa
     6361 aaaagatata attccataca aaactagtgg agggcttgat acagttgcaa taaggatgcc
     6421 ttctaatgtt attgccaggg caataataaa agaagctggt atacctattg cagctccttc
     6481 tgcaaatatc tcaggtcgcc catctccaac aaaagcaaaa catgtatatg aagagatgaa
     6541 tggaagagtg gatggaatag ttttaggagg agacagtaat tttggattgg agtcaactgt
     6601 attggattta acagaagaga ctcctatgat actgagacca ggaagtataa ctaaagaagt
     6661 tttagaggaa cttcttggag aagtaaagct tgacccatca ttaagtaaaa aagaagataa
     6721 tcaaaaagct aaagctcctg gaatgaaata taaacattat tctccaaatg cagatgtata
     6781 tataatatct ggaaaaagag aaaatgtagc aacaaagatt aatgatatga taaaatctaa
     6841 tagagacaaa ggtctaaaga caggtgtaat gtgtattagt agaaaccgag agttttatga
     6901 tggagaagta atcgatttag gaaactctct tgaagaagtt gcaagtaatc tgtttgatgc
     6961 tcttatagaa atggacaaaa agggtgttga tgtcatatat tcagaagagt tccctaagac
     7021 tggagttgga caagcaatta tgaatagact actgaagtca gcaggataca aagtgataaa
     7081 ggcttaagag ggagttgtaa aatatgaata tacttattgt gtgtacaggt aatacttgta
     7141 gaagtcctat ggctgaggct atactaagga aagcaattaa agaatctgga agaagtattg
     7201 aagaatattc tatatcatca gcaggtatat ctacagcaaa tggaatgggt gcttctgaaa
     7261 attctataga agtactaaaa gaaatcggta tagacttatc aaatcataga agtaaagtaa
     7321 ttactaaaaa attaattgat gaatcagata taatactaac aatgactaaa tctcataaag
     7381 aaattttagt acaagcagtt ccaaaatgca aagaaaaagt gtatactttt aaagggtttg
     7441 caaataaaaa tgaggaagat atatctgacc catttggtgg aaatttggat atttatagaa
     7501 gcactatgag agaaataatg tattctgtca acgaaatagt aaaaaaaata tgaaacggag
     7561 gataaacaaa tgaagatagg attaggctgt gaccatggag gatataattt aaaaaaagaa
     7621 ataataagct atttagaaag taaaggtata gaatgtgtag attacggaac taataatgct
     7681 acagattctg ttgattatcc tgtttatgga gaaatagttg caaattcagt tataaataaa
     7741 gaagtagatt atggaatatt atgttgtggt acagggatag gaatatcttt agctgctaat
     7801 aaggttcctg ggataagatg tgcagttgta tcagatgtat tttctgcaaa aatgtcaaaa
     7861 gcacataatg atgcaaatat gttatcatta ggcgaaagag ttttaggaaa aggtctagca
     7921 cttgagatag ttgaagcatg gattaatacg gattttgaag gtgatagaca tgctagaaga
     7981 gtaaacatga ttaaatcaat agaagaaaaa cataataaat aataaaaatt acagttttat
     8041 caggaggaat tatcaaaatg agcaaagtgg tagagacaaa ccatccatta atacaacata
     8101 aattaacttt aatgagagat aaaaatactg gttctaaaga ctttagagag cttttaactg
     8161 aaattgcaat gttaatgggg tatgaaataa caaaagatat accattaaaa gatgtagaaa
     8221 tagaaactcc tattcaaaaa acatcatcta aagtagttgc aggtaaaaaa ttagcaataa
     8281 ttccaatttt aagagcaggt cttggaatgg tagatggatt agtgagctta atgccagcag
     8341 caaaagttgg acatgtagga ctatatagag acccagaaac attaaaacca gttgaatatt
     8401 attgtaagct tcctcaagac ataggagaaa gagatataat agtagttgac cctatgcttg
     8461 caacaggggg ttctgctgta gcagctatag atttacttaa gtctaaagga gctaaaagta
     8521 taaaattagc taatttagta gctgcaccag aaggtatagc agaagtgcaa aaatatcatg
     8581 atgatgttga tatatatgtt gcatcagttg acgaaagatt aaatgaacat ggatatataa
     8641 ttcctggtct tggtgatgct ggagatagat tgtttggaac taaataaaaa taaataacta
     8701 taaatattta ttagggactt tcttatatag tgtatataat tttatgtaag aaggttcttt
     8761 tttatatatt ttttctcaaa ataggaatta ttgaattgat aaatttctcg aattgagaat
     8821 aaaaagtttt taccaattaa aaaagattgc ttattttaca aggttttata gaatagtagt
     8881 ctattttaat gtggcatgtt agttgcttac gattaagatt ataggtatta agtgtattag
     8941 ttaaattgag aattgaatat taataatatt attgggagtg gtaatacatg aagaattgta
     9001 tagtagtaag aggggctgga gatttagcaa cagcagttat tcataaatta cacaacagtg
     9061 gttttaaagt agttgcgtta gaaatagaag agccattagc tataagaagg caaatttctt
     9121 tttgtgaggc tgtttatgaa aaaaaagtaa ctgttgaagg tgtagaatgt atattttgca
     9181 atactgaaaa agaaatagaa gaagttttat ctcaacataa agtagccttg attatagacc
     9241 caaaaggaga aaaattatct aaattaaatc caatcgtttt ggttgatgga atactcgcta
     9301 agaaaaactt aggtacaact aaagatatgg caccacttac aatagcttta ggtcctggat
     9361 tttgtgcagg agaagatgta gatgttgtta tagagacaat gagaggtcat aatctaggaa
     9421 gaataataac taatggtaga gcagaaaaaa atacaggagt accaggagca ataaaaggtt
     9481 tttctaaaga gagagttatg cattcacagt atgatggcat aatggaaaat gtatgtaaga
     9541 taggtgatat agttgaaaag ggagacctac tttgctatat taataattat gaaaaaaaac
     9601 atgaagttag agcaacaata tctggagtcc ttagaggttt attaaaggat ggagcaaagg
     9661 taaacaaaaa tttaaaaata ctagacatag accctagaaa agaagaagtt aagaatgctt
     9721 ttacaatatc agataaagct agatgcatag ctggaggggt tttagaagcc atactaaaca
     9781 atgggattta tccccaaaat taatttatat tagataaagc tattaatatt ttattaagat
     9841 gatataataa atttaataga ttaaagagag catatgacgg gtatttaaaa tttaaatggt
     9901 aaaaatgctc tttttttatt ggacattcaa ttaacatgtg tatataatat aaaaataagt
     9961 atttatttag gttgaaatct aataaatata taataccagt acataagcaa agaggagaaa
    10021 agtaaattat gagaccatca tgggatgaat acttcatgga aattgcagaa gtagtgaaaa
    10081 agcgttcaac ttgtattaga agacaagtag gagctgtaat tgtaagagac aaacaaatat
    10141 taacaactgg atataatggt tctccgagaa atcttgaaca ctgtgaaaat attggatgta
    10201 agaggcagga attaaatatt ccctcaggag aaaggcatga attatgcaga gcattacatg
    10261 ctgaacaaaa tgcgataata caagctgctc ataatggtat tagtgtagat ggggcaactt
    10321 tgtatgttac tacaagacct tgtgttttgt gtgcaaaaat gtgcataaat gcaggtatat
    10381 tgaaaattgt ttatgaaggt gattatccag atgatatgtc tacagaatta ttaaaagaag
    10441 caggaataga actcataaaa ttttagtgga taaaatttat aagacaataa atgtcaaatt
    10501 atgacactga aatacaatgg tatcaaatga ttaaaagaaa gtatttgaaa aatcatcaga
    10561 ttagtagtaa gatatatttt ataattaaat aaatttatga gatagattaa ttacatgtta
    10621 tttattttat aataatgagg gatactattt atgagtaaaa gaaatacaca tacagaaatt
    10681 gcaaagttgt ttagtttaat aagtcaaata ggattgatga ttgtaatatc aatattagga
    10741 tgtactttct taggaaaatt tttagatagt aaattgaata ctactccagt tctgacaata
    10801 atatttttat tgttgggtgt tggaggtgct tttagtggtg tttataaaac tctaatagta
    10861 tatacacgaa ggaagtgata tattggatat aaaattactt aaccaggtaa ggtcagtaac
    10921 aaaaggtata gtaatatttg atgtagtttt aattgtgcta atgttgataa catcaacatt
    10981 aagtaaacca gtattattag gacttatatt tggctctata atagctatgt taaattttag
    11041 attattggcg atatcactgg aaaaatcagt gacatttcct gctgggaagg cacaagctta
    11101 tgcatcagca caatatgctg ttagattatt tataatggca gttgttctaa ttgcatcagc
    11161 tacagtacca catttaaata taatcggaac agcattggga ttattgagca caaagtttgt
    11221 catattatca aaaaacttta taaataaatt aaagaggaag gaggcataag tatgaatcaa
    11281 gcgcattatt atctcatagg aaactttaag attagtgaaa caatactagt aagttgggga
    11341 ataatggcct ttttagcgat agcatcttat tttttgacta gaaacttaaa aaaagttcca
    11401 actagtaaag ttcaaatatt cttagaattt gcagtaggtg gtcttgctaa aatggttaca
    11461 gatgttatgg gtaaaaatac agtaaaaaga atgccatata tgataccata cataggaagc
    11521 ttgtttttat ttttcgcatg ttcaaattta agcccattac ttggattaag gtcaccaaca
    11581 accgacttag acacaactgc tgcatgggca atgattacat tttttatgat ttattttgca
    11641 ggagttaaat ttaaggggtt atcatacttt aaagaattgg ctcaaccact tgccataatg
    11701 ttaccaatca atattatagg tgagttagct agaccaatat cattaagttt ccgtccattt
    11761 gggaatattt taggtggaac aataataatg ggtctgtttt atcaactttt aggttttata
    11821 tctagtttaa taccaggatt atcaatacca attggagaaa tattaatacc agtcccatta
    11881 cacttgtact ttgacttatt tgcaggattg ttacaagcat atatatttat aatgttaaca
    11941 atggtattta tatcaagtgc aggagagtta gattaagaaa tcaacttata ttaagataaa
    12001 aataaaataa tttaaaaaat taaagtttta aggaggaaat aattatggaa acagcaatag
    12061 tagcggcagc atcagcaata ggagcaggga tagcagttgc aacaggtata ggtgcaggta
    12121 taggtcaagg tatagcagca gctaaagctg cagaagcagt aggaaaccag ccagaagcta
    12181 aaggtgatat aacatcaact ttattactag gagttgctat agcagagtct tcagcaatat
    12241 atggtttggt tatatcaata atacttctat tcgtaaatcc attctttaaa tacttaggaa
    12301 tgtagattta gtccttaaat attttgagat aggtttatag gtaaatcatc tcaaacttat
    12361 aatcatgaga tgaactttat catctcgtga ttataagtag ttatgattta tggaaaggag
    12421 gataattatg gaaaaagcac tagtaggtat aacgtgggaa tttgtgttcc aaattgttaa
    12481 tacttttata atatttttac ttctgagaaa gcttttattc aagccagtat taaatataat
    12541 tgaatcaaga gaaaatgata ttaaaagtga tttggcagaa ggcgaaaaag caaaaaatga
    12601 aggtttagct ctgaaaaaag aatatgagtc aaaaatcaat tttgccaaag atgaaggtca
    12661 agaaatcata aaacaagcaa ctataagagc agagcagaaa tcagatgata tagtaaatac
    12721 tgcaaagaaa gatgctcttg atataaaaga aaaagcaaac aaagatatag aacaagaaag
    12781 acaaaaggtt ataaatgaaa tcaaaaatga tatatctaac atagcactac ttgcagcatc
    12841 taaagttatt gaaaaagatt tagataaatc taaacatgaa gaattgatag aaaactttat
    12901 aaaagaggta ggggaagcta aatgataaat gtaatagcaa atagatatgc cgaagcccta
    12961 tttcaattag gagaagaaga aaattcaact gatgtcttat tcaaagaact agaaaaagta
    13021 gtagatatga tgactaaagt tagtaaagat ttctacaaag ttttaaaatc accattagtt
    13081 tcaaaatcag aaaagaaaaa cttggtagaa attattttta gtaaggaagt aagttctaac
    13141 ataaaaaact ttttaaaagt tttagtagat aaagatagaa tatcatattt agaagatatc
    13201 gaattagctt ataaagaatt attaaataag aaaaacaatg taattgatgg agtagcaata
    13261 agtgctattc caatgagtga aacagatatt aaagaacttg aagtcaaact ttcaaataaa
    13321 tacaacaaaa atgttactat agaaaatgtt gttgacaaga ctatactagg gggagttcta
    13381 gttagaattg gaaatgaaca aatagatggc actgtgaaaa ctcgtcttga taagatgaaa
    13441 gaaaagctat ctgaagtaat atcttaagaa tggcggtgaa cccatgaact taaaacctga
    13501 agaaataagt tctataatta aacagcaaat aaaaaattat gagaataaag ttgagttgac
    13561 agatacaggt agtgttttaa cagttgggga tggtatagcg agtgtatatg gattagaaaa
    13621 agctatgtct ggtgagttgc tagagttccc aggtgaaata tatggtatgg cacttaactt
    13681 ggaagaagaa gtagttggag ctgttatcct tggagatgac agtgaaataa aagaaggcga
    13741 tatagttaag agaactggaa gaatagttga agttccagtt ggagaagctt taataggtag
    13801 agttgtaaac tcattaggtc agccaataga tggaaaagga cctatagcgt acacaaaaac
    13861 tagaccagtt gaatctgagg cacctggtat aatagataga aggtctgttt atgaaccatt
    13921 acaaacagga atcaaatcaa tagattcaat gataccaatt ggtagaggtc aaagagagtt
    13981 aataataggt gacagacaaa ctggtaaaac atctatagtt attgacacta tactaaatca
    14041 aaaaggaaaa gatgttatct gtatatatgt agcaattgga caaaaacgtt ctactatagc
    14101 tcaattagtt agctctttag aaaaaggtgg agctttggat tatacaatag tagtttcagc
    14161 aacagcatct gaatcagcac cacttcaata tatagcacca tatgctggag cagctatggg
    14221 tgaagaattt atgtacaatg ggaaacatgt acttatagtg tatgatgatt taagtaaaca
    14281 agctgttgca tatagagaaa tgtcattgtt acttagaaga ccaccaggac gtgaggcata
    14341 tcctggagat gtattctatt tacattcaag attacttgaa agagcagcta agttatctga
    14401 tgaattgggt ggaggttcaa tgactgcctt gcctataata gaaactcaag ctggtgacgt
    14461 ttctgcatat ataccaacaa atgttatatc tataacagat ggtcaaatat acttacaacc
    14521 agagttattc tattcaggtg taagaccagc agttgaccct ggtatatcag tatcaagggt
    14581 tggtggttct gcgcaaatta aagcaatgaa aaaagttgca ggaacattaa aacttgcata
    14641 ttcacaatat agagaacttg cagcattctc acagtttggt tctgacttag atgaagatac
    14701 taaaaagaga cttgctcaag gtgaaagaat cgttgagata ttaaaacaag gtgagcatca
    14761 accaataaaa gttgaaaatc aagttatgat aatatatgct gttataaata atcatttaga
    14821 ggatattccg atagataata tagctagatt tgaatcagaa ttgtatgcat ttgtagataa
    14881 taattatccg gaaatatcaa gaaaaatatt aggtggagaa gacttcactc atgatttaac
    14941 agatgcaata aatgaattta aagaaaagtt tgttgttgaa gtataatcct ttttaaaaaa
    15001 ggaggtaaat taattggcag gagctggaat caaagcaatc aaaacacgta tgggaactgt
    15061 tgaaagtaca aagcaaataa ctaaagcaat ggaattagtt gcatcttcta agcttagaaa
    15121 agcaaaagaa aaggctgaag attcaagaga atattttaat actttataca atacagtaaa
    15181 agatattgcc caaacaacac gtggaattag aaatgaattt ttaaaagaaa gagaagtaaa
    15241 aaataaatgc tatgtagtag tagctggaga cagaggactt gcaggtggat acaattctaa
    15301 tgtattaaag gaatctgtct ctcatatgaa aggtaaaaag gaaacagtga ttactgttgg
    15361 aaagaaagca catgaatttt tttctaaaag aaactacaat atagcaaaag atataaatag
    15421 tgttgaagct tgtggatatg aagatgcgac agaaatttca caaactatta ttgacctgta
    15481 taaaaaaggt gaagtagatg aagtttatat tgcttataca aaatttaaat ctgctttatc
    15541 acaagaagtt gaaattttga aattgcttcc attagtcttt gaaaaagata cagacgaaca
    15601 aggaatggca gtaaaagaag aaaaacaaaa tagatcaaaa gtacaatata taccatcagc
    15661 agaagcagtt ttaagttata ttgtacctca gtttatatca ggtacagtat atggtggaat
    15721 tttggaatct tttgcatcag agcaaggtgc tagaagaact gcaatggaat cagccacaga
    15781 caatgcgaat gaaatgctat ctaatttaga gttaagttat aacagagcaa gacagtcagc
    15841 tgtaactcaa gaaataacag agatagttgg tggagttgaa gctctaaaat agggaggtta
    15901 ggaaatggca aatgtaggta aagtagtaca gattgtagga gctgtactag atgttaagtt
    15961 tgatggtgaa caaagcctac ctaacttatt aaatgcgtta gtaataaagt taggagataa
    16021 agaaatagtt gcagaagttg ctcaacatat aggtgatgat actgtaagat gtatagctat
    16081 gagtgcaaca gacggacttg taagaggtat ggaagttgta gatacaggag ggccaataag
    16141 tgttcctgtt ggagatgaga ctttaggtag aatattcaat gttcttggaa aaccagttga
    16201 tgggaaacca gctccaaaat ctgcacctaa attacctata catagaccag caccggcgta
    16261 tgatgaacta gaaactacag cagagatact agaaactggt attaaggtag ttgacttact
    16321 agctccatat ctaaaaggtg gaaaaatagg tctatttgga ggagcaggag taggaaagac
    16381 agttctaata caagagctta taaacaatat tgctaagcaa catggtggta tttctgtatt
    16441 ttcaggagta ggagaaagaa caagagaagg taacgacctt tatggcgaaa tgagtgagtc
    16501 tggagttata aataaaacag ctctagtatt tggtcaaatg aatgaaccac ctggagcgag
    16561 aatgagagtt gctttaactg gacttacaat ggcagaacat tttagagatg agcaaggaca
    16621 agacgtttta cttttcgttg ataatatatt ccgtttcaca caagctggtt cagaagtttc
    16681 agcacttcta ggacgtatgc catcagctgt tggttatcag ccaacattag ctactgaaat
    16741 gggtgcactt caagagagaa taacatcaac taagaaaggt tcaataacat ctgttcaagc
    16801 agtatatgta ccagcagatg acttaactga cccagcacca gctacaacat tctctcactt
    16861 agatgcaaaa acagttttat caagacaaat atcatcactg ggtatatatc cagcagttga
    16921 ccctcttgaa tctacatcaa ggattcttga ccctagtata gtaggaaaag aacattatga
    16981 agtagctaga ggtgtacaat caatacttca aagatataaa gaacttcaag atataattgc
    17041 tatacttggt atggatgaat tatctgatga agataagtta atagttgcac gtgctagaaa
    17101 gatacaaaga ttcttatctc aatcattcac agttgctgag caatttacag gaaatccagg
    17161 tcaatatgta cctgttaaag agactgtaag aggatttaaa gaaattcttg aagggaaaca
    17221 tgatgactta ccagagtcag cattcttatt tgtaggaaca atagaagacg ctgttagaaa
    17281 agcgaaggga agtatgtagt catggccagt gaattttcat tgaaagttgt aactccaagt
    17341 aatacttttt atgatggaga tgttgaaatg atagtagtta gaactacaaa aggagacaaa
    17401 ggggtgttga aaggtcatat accatttgtt tcaggtatac ctgtaggaac tttaaagata
    17461 aaaaaagagg gaagttttaa agaggcgaaa atagcagaag gattcattaa tgtaactcaa
    17521 gataaaactg tcatattaac agaatctgct gagtggattt aataaattaa tttagagtat
    17581 aaaaagtcat tttgaccaaa aaaacttttg ggtaaaatgg cttttttgtg ttttggtgtt
    17641 aataaaataa cgataaaatt gtaagttttt agtgataata aattcacaaa atgtttaaat
    17701 ttactgccaa attatgttgt aattaataag ataaagaaga tttagatagc ttttatgtaa
    17761 gtgaaaaagt cttcatataa gaaatacata taaaattttt ataaaattta aaaaatttgc
    17821 ttagttgtgt agta
//
LOCUS       DAEOSP010000061        17608 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.061, whole genome shotgun sequence.
ACCESSION   DAEOSP010000061 DAEOSP010000000
VERSION     DAEOSP010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17608
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.061"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            43..705
                     /locus_tag="KRH54_001731"
     CDS             43..705
                     /locus_tag="KRH54_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBG8579396.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            1076..2485
                     /locus_tag="KRH54_001732"
     CDS             1076..2485
                     /locus_tag="KRH54_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBG8579397.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            2600..2875
                     /locus_tag="KRH54_001733"
     CDS             2600..2875
                     /locus_tag="KRH54_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBG8579398.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            3505..3816
                     /locus_tag="KRH54_001734"
     CDS             3505..3816
                     /locus_tag="KRH54_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579399.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            3818..5743
                     /locus_tag="KRH54_001735"
     CDS             3818..5743
                     /locus_tag="KRH54_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBG8579400.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFNCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            5746..6237
                     /locus_tag="KRH54_001736"
     CDS             5746..6237
                     /locus_tag="KRH54_001736"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBG8579401.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            6289..6852
                     /locus_tag="KRH54_001737"
     CDS             6289..6852
                     /locus_tag="KRH54_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBG8579402.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            6867..7844
                     /locus_tag="KRH54_001738"
     CDS             6867..7844
                     /locus_tag="KRH54_001738"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBG8579403.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            7831..8154
                     /locus_tag="KRH54_001739"
     CDS             7831..8154
                     /locus_tag="KRH54_001739"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBG8579404.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            8271..10049
                     /locus_tag="KRH54_001740"
     CDS             8271..10049
                     /locus_tag="KRH54_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBG8579405.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            10046..11419
                     /locus_tag="KRH54_001741"
     CDS             10046..11419
                     /locus_tag="KRH54_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBG8579406.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            11425..12093
                     /locus_tag="KRH54_001742"
     CDS             11425..12093
                     /locus_tag="KRH54_001742"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBG8579407.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            12370..13419
                     /locus_tag="KRH54_001743"
     CDS             12370..13419
                     /locus_tag="KRH54_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG8579408.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            13515..14504
                     /locus_tag="KRH54_001744"
     CDS             13515..14504
                     /locus_tag="KRH54_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8579409.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKGKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDASIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            14509..16227
                     /locus_tag="KRH54_001745"
     CDS             14509..16227
                     /locus_tag="KRH54_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBG8579410.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAASLAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(16309..17463)
                     /locus_tag="KRH54_001746"
     CDS             complement(16309..17463)
                     /locus_tag="KRH54_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG8579411.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
BASE COUNT         6729 a         2163 c         3196 g         5520 t
ORIGIN      
        1 aaaaacttta ataaaaattt agtgtatagg agttgtttaa gtatgataga tataagtatt
       61 gatgacaaac ttaaggaaag atggcctaat gctaagctgg gttgcattca agcaaaagta
      121 gcagtaaata aaagctcaga aaaattaatt aatgaaataa atgagttttg tgatactata
      181 aatcaaagtc taaaagttga agacattacg aaactagata aaattaaaga tgcaagaaat
      241 gcttataaag agcttggaaa aaatccaagt agatatagaa catcttcaga agcattggtt
      301 agaagaatta ttcaagggaa gggtctttat acaataaata atatagtaga aataaacaat
      361 ttaatatcaa taaaatcatt atatccagta ggtacatata atgtaagcaa attacattca
      421 ccagtatgtt ttactgtagg agatgaagga gaacagtata agggtattgg gaaagaatta
      481 ataaatatag aaaatcttcc tatattatct gattcagtgg gtaaatttgg gagtccaact
      541 agtgattctg aaagagcaat gataacagaa gatgcaaatg aaatattaat ttgtatattt
      601 tcttttagtc aagaatctga tattgaagaa tattttggat atgctaagga attacttaaa
      661 gaatatgcag atggaaaaga tatagaaact aaaataatta aatagaatct atataacaac
      721 gttatgttaa gctaaaattc aatataaaaa taagttaaga taaagaaact aacacaaaat
      781 tagtttcttt tttaaaatag tagtttagtg ttacaaaaat agatagatag tcccacgatt
      841 attaaaagta tggtaatata ttaatatagc agagattgtg tttaagggat tttgaaaggg
      901 gggatttata ataatcattt tatctaaaag ataaaatggt tttatagaaa taactatata
      961 tttaaaaata taaataatga tacagtaatt tgggtatata aaataaggca tacatggttt
     1021 tgtttaaaaa ccaataaggt acataggtaa ttacaatgta aggaaggatt taattatgat
     1081 tgatggaaaa gaggtaaaac aagaaagtgg acaaaaaaat ctaaaattta agatagctat
     1141 agtagtaggt gttttgtttg tagcttatat aggaatggca atctttttta gaaatcattt
     1201 ttactttgga acgactatta atggcgtaaa ggcttcagga aaaacagttg agcaagtaaa
     1261 tgatttgtta acatctagta ctaattccta tactttaaac ttagaggaaa gaaacaataa
     1321 gaaagaacaa ataaaagcaa aagatattga tttaaaattt acaccagatg ataaagttca
     1381 aaagataaaa gatagtcaga gcgctattgg atggattggt ggaatattta agtctaaaga
     1441 gtataataat atgataactc tttcatataa taaagattta ctagaaaaaa ggtttaatgc
     1501 tttatcttgt tttgatagta aaaatgttgt agaacctaag agtgcttatc ctaagtatga
     1561 tagcactaaa aacgcttatg ttatagtacc agaggtactt ggaaacaaag tgaaaaaaga
     1621 tgatttttat aagttagtac aaaactcaat taattctggg aatacttcta tttctctgga
     1681 tgaaagtaaa gtttatgaag caccaaacaa cacaaaggat tcagaaaagt tgaaacaagc
     1741 tattaagaca ttagataagt atgttggtgt agtcgttaca tatgatttta gtgaccgtaa
     1801 ggaaacttta gatggttctg aaattaataa atggttaaaa gtagactcag aaaagaatta
     1861 tgacataact tttgataatg atgctatggt agaatataca agaggtttaa gtaaaaaata
     1921 cagtactttt ggtgactcaa gaccatttaa aactgccaca ggtaaagata ttacaatttc
     1981 aggtggaatt tatggttggt tgatagataa aaagaaagaa gctgaagcat tagttgatgt
     2041 tgtaaaagct ggtaaaaatg ttacaagaga acctatttac atacaaaaag ctgtatctcg
     2101 taataaagat gatattggaa atacatatgt agagattagt ctttctggcc aacatatgtg
     2161 gttctttaaa aatggagaaa tgcttgtatc aacagacgtt gtaacaggtg atttagctag
     2221 aggatttgct acaccatcag gtatatatcc tcttaattat aaggctagaa atgtttcatt
     2281 aactggtcaa ggttacagtt cacctgtaag ttattggctt ccgtttaatg ggaacatagg
     2341 cattcatgat gctacttgga gaaattcttt tggtggaagt atatataagt cttcaggttc
     2401 acatggatgt gtaaatacac ctttttctaa ggcaaagaaa atatatgaaa atatagaacc
     2461 aggtacacca attattttat actagtaaat gtgttgctaa cagttaagcg aagtcatctt
     2521 agtttattta ggatggcttc ctttttatat aaagcttatt gaagtttaca gaaagactac
     2581 ttaaaaggag aaatacataa tgaatgaaaa attaactaaa attttagaag aagctaagaa
     2641 tgatgatgaa tttagaaaag aacttataaa aacaagagaa caacgtgatg ttatggattc
     2701 attttgtaag gttgttacat ctagaggtta tgatatgaca gtaggggaac ttttttctat
     2761 gggagaagaa tatacatcca atctactgaa aagtgtaaat ggaggagcag catatcctat
     2821 tgaagattgg agtgattggt acgaggattt ttttataatg ttgtcgaata tatagagtaa
     2881 ctaatgatat tatctactta agattaatac attgccttaa aataaaacta ttagaactag
     2941 gtgtaagttt tatcaatctt taaaaatatt gatttatttc taactagata taaaaaaatt
     3001 agattaaaaa taaggtgaca aaaatcgata tagcttgatg atttataaaa gttagataaa
     3061 atagtgcgag atatttctaa aaattcaaaa aaaacaataa acttcaatgg ttagaggttg
     3121 aaaaagaata taaaaaactt ttatatgtaa taaattaaat taaaattata aaaaaaataa
     3181 aataaaaatt taaaatgtcg acataacgtt aattttaaaa caaaataacg ctattgacaa
     3241 actatatcgc tttaaattat aattattgaa aataatggta tttaaaaacc atactcatgt
     3301 catagtctaa aagtaagtca aaaaaggatg taaatctagg caagtactat tctaaaatat
     3361 gttttattaa gaaataaacg ttgaaaaata ttaattatta tataaaaatg ctgtttatac
     3421 tttaacaatg aggtagtaat ctaattagaa taaaaaaatc taattggtta tatacatagt
     3481 aaaaaggtaa gagaggtgat ttggatggct tttgaaataa taaaaagcat tgttgaggca
     3541 gagcagacag cagacagtat caaagtaaaa gctgttactg atgcagagtc tatcagagct
     3601 gatgctgtaa acaaatgtga aagcatattt gctgatgtaa aaaaacaagc aaagcttatg
     3661 gaagaaactc ttattgagaa ggcagtcacc gacagtagag cagaggttga taaaatctta
     3721 gctaatgcta aaagtgaatg tctgaaaatt gaaaaaactg ctgaagaaag aaaaagtaag
     3781 gctattgaag cggttattgg aaaggtagtg agataatatg gcaatagtta atatgaacaa
     3841 aatatctatt gttggcttag aagctcaaaa aagccagatt ttaaagctac ttatgaaccg
     3901 tgggtttgtt cagattgatg atagtgcctt tcttactgag gaggaggaat taaaaaatta
     3961 tcttgtaaaa gatagtgaag aatctgaagt tataatgcta gagcagaaaa tgtctcatgt
     4021 aaaccaagca attaatattg tttcttcact agtgaaacaa aaaaaatccc tacttgcacc
     4081 aaaaagagag tttaacaaaa ttgatagtga aaaagaaggg caagtttatg ctgaagctct
     4141 ggagttaaac aatttgcaca aggagatagg tacaataagg tctaacataa acttacttaa
     4201 caacaataaa agtatgttag aaccatgggt gaactttgat attcctcttg aaaatatgga
     4261 aactaagtat acaaaaacta tacttggaac tgtgcccata agcgcaaaca ttgatgcttt
     4321 aagagtaaaa ttagaagatg aaataccaga aagtgttatt gggattgtca attctgacaa
     4381 gttaatgaac tatgtttatt taattacttt aaaagaaact tttgatgatg ttttagaagt
     4441 tttaaaagaa tttaattttt cagtggtttc attaccaaat gaagaaggta caccgttaca
     4501 agctatcaag aaatacaatg aaatgataat tagacaggaa aacgtttcta aggacaaaac
     4561 agaggaaatt aaaagacatg cagatagtat acacatgctt gaaaacctat atgattttta
     4621 cactatagaa agagaccaaa aaaagattat agagagactt gtcaaaacaa aaacaacatt
     4681 ctgtcttaat ggttggttac caagcaaaag ggcagatgaa ttaattaaag aaattacaga
     4741 aaagtttaat tgttgtgttc agactgaaga aggaagtaaa gaagaaggct tcccaattct
     4801 gcttgaaaac aataaattag taacaccttt tgaaagtgta actaacatgt acagctatcc
     4861 aagtacaaaa gatattgacc caaatacaat tttaactttc ttctttgttg tattctttgg
     4921 aatgatgcta agtgacgccg catatgggtt aataattgca gtagtctgtg gatttgttgt
     4981 atataaatta aagatacaaa aaggtgaagg aaatttaatt aaacttattg gtatctgtgg
     5041 agtttcaact actgtatggg gtcttatatt cgggggtatt ttgggggatt taattcctat
     5101 aaaagcatta attaatcctt tagaggatgt tatgacactt atgggaatgt cgttactgtt
     5161 cggtattatc catatatatg taggtttggg aataaaagca tatacattaa taagaagtgg
     5221 aaaggtaata gatgcaatct ttgacatagg tttttggtat ttgtgtataa caggtgtatg
     5281 tctacttata ataccttttg ttgcagggga tataggtgta ttttcagaag taggaaaata
     5341 tttagcaatt ataggtgcta taggtcttgt acttactcaa ggaagaagct ttaaaggtat
     5401 tcctgttaag ctttttaaag gattttcaag tctctatggt ataacaagtt attttgctga
     5461 tatattatct tatacaagaa ttatggcgct ttgtttaaca acaggagtta tagcacaagt
     5521 tatcaacctt ttaggagcta tagctggtcc gatacttgca gttgtaattg gtgttgtagg
     5581 acacacaata aaccttctta taaatgcact aggtgcatat gttcatacaa gtaggttaca
     5641 gtatgtagaa tttttcaata agttttatga aggcggagga gttccttttg ttccgtttaa
     5701 gtataaaaca aaatatacaa gcattaataa aaaggagatg taaatatgga atttttaaac
     5761 gcgttaggaa atggtcaatt tttggcaata tcaggagctg ctatagcagc attatttgca
     5821 ggtatgggtt ctgctaaagg tacttcaata gcaggtcaag ccgcagcagg tgttgtaaca
     5881 gaagacccta gtaaattcgg tcaacttcta gttttacaac ttcttcctgg tacacaaggt
     5941 ctgtatggat ttatcatagc tttccttatt ttatcaaaag caggaattat tggtggagat
     6001 gcaataccaa catcagctca aggtttacaa cttcttatgg caggacttcc aattggttta
     6061 gttggtttag tatcaggtat agcacaaggt aaagcagcag cagcaggagt tggaatagtt
     6121 gcaaaaagac ctgaagaact tggtaaagct ataacatttg cagcaatagt tgaaacatat
     6181 gctcttttag gactacttgt ttctttctta gcttacaatg gaattgcaat tggttaatta
     6241 taaattataa ttcataagta aaaaaaatga aaaggaggaa gcactcctat gggtaatgaa
     6301 caaaaaatga ttgacagaat tatagctgat gccaaacaag aagcacaaga aatattggat
     6361 aaagctaaaa gtgaagcaga tttaaaagta aattcagcta atgaaaaagc tgaaaaggaa
     6421 atggcttcct atacaaagct tgcagaggca gaagcagaaa aagcggcttc taaggaaata
     6481 tcaggagctt atatggaggc taaaaagcaa atattatcaa aaaaacaaga gattttagaa
     6541 gaggtaattt tagaagctaa aaacaaactt ttaaatctta aagataatga atatgaagag
     6601 attatactaa acatgattga aaaatcaaat tgtacagatg attcagagat agtattatct
     6661 aaaaaagata aaaaaacatt aaaagatgtg ctatcaaaaa aaggcattaa agtgtctgat
     6721 gaaactagag atattactgg aggtttcata gttaaaaaag gtgacattga gtacaattat
     6781 tcatttgaag ccataattgc agtagagcat gaatatattg aacaaattgc agcagaaatt
     6841 ttgtttaatt aaggaggggt taaaaaatgg cagaagaaaa aacttatccc tatgccgttg
     6901 cacgaataag agtgcttgaa aaacagcttc ttaacagaca aatgtttatt caaatggcag
     6961 aagccaaaag ccctgaagac tctttaagaa ttatagctga ggcaggttat gctgatacat
     7021 caaataataa tatacataat tttgagaata ttttgactca agaattatca aagacatatg
     7081 aaattcttaa aagtctagct cctgaagaaa aatttgttga tgtatttctt tataaaaatg
     7141 actatcacaa tttaaaagtg ctgattaaag aagaaatttc tggtgtagat ggtgagaaat
     7201 atttaattga tggaggtaca attcctctta ttaagcttag agaatcttta gcaaatcgta
     7261 gttttagtga tttacctaag ataatgtcat cagctatatt agaggcattt gatgtttata
     7321 ataagacaca aaatggccag atggttgata ttgttctaga taaagcaacc tttaccagta
     7381 tgaaagaaac tgctaaagaa agccaaaaca aatttgtaat agattatgtt atgaaagttt
     7441 gtgatttaac aaatctaaag agttttataa gagtcaaaaa tatgaagaaa gattttgata
     7501 tgtttatgaa tgtgtttgtt tcaggtggct cacttgataa ggaaaaattc ttagaagctt
     7561 tttcctcaga tactccagca tcatgtttta aatctacttc ttactctgat gtctgtaaaa
     7621 atggcatgga tagtggtttt actgtctttg aaaaactttg tgatgactat cttatggaat
     7681 atgttagagg agcaaaattt aagcctctta cattagaacc actaattgca tatttatatg
     7741 ctaaagaatc agagataaaa acaattagaa ttattttgac tagtaagctt aataatatag
     7801 acgcagatac tataaaagaa aggctaaggg atgcttatgt ataaggtagg agtagttgga
     7861 gataaagata gcatcatggg atttttagct cttggaatag atatcttccc agcctatgat
     7921 agtgacgaaa ttaaaaaaag tatacataag ttggttgaag atgaatatgc gataatttat
     7981 attacagaac aggcatcatt gcttgcaaaa gaatcaatag caaaatacaa agattaccaa
     8041 cttcctgcca ttatagttat tccaggtatt ggaggaagta tgggattagg tatgaatgaa
     8101 gtaagagaat ctagtaaacg tgctataggt gcagatatat tatttaaaga atagatgaaa
     8161 gttgaataaa agacaatctc aaattttatt ggtgctaaat atatgtgcta ataaaatata
     8221 ggagggaata tttaaatttt gataaagtat atgattgcag gtgagagaat atgaaaacag
     8281 gcacaattgt aaaggtgtca ggaccgcttg ttgtagcgga gggcatgaga gatgcgaata
     8341 tgtttgacgt cgtaagagtt tcagataaac atcttatagg cgagattata gaaatgcatg
     8401 gagataaagc ttctatacag gtttatgagg aaacttccgg tctaggacca ggggaagaag
     8461 tagtttctct tggaatgcct atgagtgttg aacttggacc aggtcttatt tcaactattt
     8521 atgatggtat tcaacgtcca cttgaaaaaa tgtatgatat ttcaggtact aatataacta
     8581 aaggtgttga agtatcttca cttgatagaa caactaagtg ggaatttaaa cctaaaaaat
     8641 ctgttggtga taaggttgta gcaggagata taattggtgg cgttcaagaa acagcagtag
     8701 tagaatgtaa aattatggtt ccttatggag ttaagggtac tataaaagaa atatattctg
     8761 gtgaatttac tgttgaagat acagtttgtg ttattacaga tgaaaaaggt aatgatatac
     8821 ctgtaactat gatgcaaaaa tggccagtta gaaaagaaag accatataga gaaaagaaaa
     8881 caccagattc acttcttgtt acaggtcaaa gagttataga tacatttttc ccaatcacaa
     8941 aaggtggggt agcagctata ccgggacctt ttggaagtgg aaaaactgtt acacagcatc
     9001 agcttgctaa atgggcagat gcagatatag ttgtatatat aggctgtggc gagcgtggaa
     9061 atgaaatgac agatgttctt cttgaatttc ctgaattaaa agacccaaga acaggcgagt
     9121 cacttatgca aagaactgtg cttatagcaa atacatcaga tatgccggtt gctgcacgtg
     9181 aagcttctat atacactggt attacaatag ctgaatattt tagagatatg ggatatagtg
     9241 ttgcacttat ggcagactct acatcaagat gggctgaggc tcttagagag atgagtggtc
     9301 gtttagagga gatgcctggt gaagaaggtt atcctgcata cttaggttca cgtcttgctc
     9361 aattctatga gagagcagga aaggtaaatt gtctaggtat ggatgaaaga gaaggaacac
     9421 ttacagcaat tggtgcagtt tctcctcctg gtggtgatac atcagaacct gtcagccaag
     9481 caactcttcg tatagtaaaa gtattctggg gtcttgatgc atctttagca tataaacgtc
     9541 atttcccagc aattaactgg ttgactagtt attcactcta tcaggaaaaa gttgatgtat
     9601 gggcagataa aaatgtaaat gataatttct cagctcaaag agctcaagct atgaaacttt
     9661 tacaagttga atctgaactt caagaaatag ttcaattggt tggggttgat tcactttcag
     9721 atggagatag attgatacta gaggttactc gttctataag agaagacttc cttcatcaaa
     9781 actctttcca tgaagttgat acttatacat cacttcataa acaatataga atgatgggac
     9841 ttatattaaa attctataag tcagcacaag atgctatcaa gcaaaatgtt actataaacg
     9901 atattatcaa gctttcagtt cttgaaaaga ttggtcgtgc taagtatgta gaagaaggcg
     9961 atatagatgc agcttacgat tcaattgaag atgaaataga caacgagctt gcagacctta
    10021 ttaaaaagag gggggctgaa gaactatgat aaaggaatat aagtcaatac aagaggtagc
    10081 cggtcctctt atgttaataa acggcgtaga aggtgtaaaa tttgatgaac ttggagaaat
    10141 tgaactttca aatggagaaa ttcgccgttg caaagttcta gaggtaaatg gagatactgc
    10201 acttgttcag cttttcgaaa gctcaacagg tataaacctt gctgaaagta aagttcgttt
    10261 tttaggaaag agtcttgact ttggagtttc accagatata ttaggtcgtg tttttagtgg
    10321 aatgggtaga cctattgatg gaggtccaga aattatacca gataaaagac ttgatataaa
    10381 tggagctcca ataaatccag cagcacgtga ttatccggcc gagtttatac aaactggagt
    10441 ctctgctata gatggactta atacattggt tagagggcaa aaacttccta tattctcagg
    10501 gtctggtttg ccacatgcca atcttgcagt tcagattgca agacaggcaa aagtacgtgg
    10561 aactgattct aagttcgccg ttgtatttgc tgcagttggt ataacttttg aagatgctga
    10621 attttttata agcgatttta aagcaactgg tgcaatcgac cgttctgtag tgtttgtaaa
    10681 ccttgcaaat gacccagctg tagagcgtgt atctacacct agaatggcac ttacagcagc
    10741 agaatacctt gctttcgagc aagatatgca tgtacttgtt ataataacgg atattacaaa
    10801 ctatgcagag gcgttgcgtg aagtttcagc agctaaaaaa gaagttccag gacgtcgtgg
    10861 atatccagga tatctttata ctgaccttgc tactatgtat gaaagagcag gaaaaatgaa
    10921 agggcatgaa gggtctataa ctatgattcc aatacttaca atgcctgagg atgataagac
    10981 acatccaatt cctgacctta caggatacat tacagaaggg caaattatac ttagccgtga
    11041 actttataaa aaagatatac agcctcctat agatgtttta ccatcacttt ctcgtctaaa
    11101 agataaaggt attggtaaag gtaagactcg tgaagaccat gcagatacaa tgaaccagct
    11161 ttttgcagct tattcaagag gtaaggatgc aaaagaactt atggctatac ttggtgaatc
    11221 tgcactttct gagattgatt taatgtatgc taagtttgca gatgaatttg agaaagaata
    11281 tgtttcacaa ggatttagaa ctgatagaac tattgagcag acattagaaa tcggatggaa
    11341 gcttcttagt atgttaccaa aaagtgaact taaacgtatc cgtgatgaat attttgaaaa
    11401 atatatgcct aaggagtgat atctgtggct agattaaata taaatccaac acgtatggaa
    11461 atgactagac ttaagaaact ccttaaaact gctacaagag gtcataagct tcttaaagat
    11521 aaacttgatg aacttatgaa acaatttctt gagattgtaa gagaaaataa gcgattgcgt
    11581 gaagaagcag aaaatgctct tgatactgct tataaaaact ttataatcgc aagagcagtt
    11641 atgagtcagg agtatctggg ttcagcactt atgatgccaa agcaatcagt ttcagtagat
    11701 gtttctacga gaaatataat gagtgttgat gttcctgtgt ttgactttaa aactgaaaac
    11761 aatcaaagtg atatttatcc ttatggtctt gcttttacat caggtgaatt agattctgct
    11821 atggaagctt tctcagatgc tatgcaacca cttttaagac ttgcagaaag tgaaaaaagt
    11881 gcacagcttc ttgcacaaga gatagaaaag acacgtagac gtgttaatgc tcttgaaaat
    11941 gtaatgatac caaattacat tgagactatt aagtatatag ctatgaagct tgaagaaaat
    12001 gagagagcaa gtactactag acttatgaag gttaaggata tggtgcttaa aaaagcactt
    12061 gaagaaaaga aaaaaaatga tttggttgta taattaaata cttataaaaa taagtctaac
    12121 attctacttt atagaggtta gacttatttt ttatgtttaa attaaaatgt agatactata
    12181 aaaattaata tattgtttaa aaacagactg tatttaacta aaaataatat atttaacaaa
    12241 aatttctaaa aattaacgaa ctgttaattt acacttaact ttatattaac aaattaggtg
    12301 tattatataa acataagcaa aaacaataaa aaacaaataa aaaaaggaaa aaacacaaag
    12361 ggggaagata tgaaagaatt tatatttact tgtctgtgca ttgtagtgat tacatttatg
    12421 acaagttgca tatcaacaac accacaaact actcaaggaa aaggaaataa taaacttaca
    12481 atatatgcag cactagagga agagcagata gcttattata tggaaggttt tagagaagag
    12541 tatcccaata tagatgtaga gatagttatg gattctcatg gtgtaatagc ttcaaaacta
    12601 ttagcagaaa aagataatcc acaagcagat attatatggg gattatcagc aatgaacatg
    12661 atagagctaa aaaaagatgg tgcacttgaa gcatataagc ctaaaaatta tgataaagtt
    12721 gataaaaaat ttaaagatac tgaagatgat gtatcttggg taggaatgag tgtcccagag
    12781 acagcaatag tagttaatta taaggaactt aagaaactag gtatagatat tccaaaaagt
    12841 tatgaagatt taataaaacc agaatataaa ggacttataa caatgccaaa tccagcatca
    12901 tctggaacgg ggtatcttac agtatcaggg cttattcaaa tgatgggtga aaaagatgct
    12961 tacaattaca tggataaatt acataaaaat atagccacat atacacactc gggttcaaaa
    13021 cctgcgaagt tagctggctc tggagagtat ccaataggta ttacacttgg atatagagct
    13081 acacaacaat tatctagtgg agaacctata gaggttgtat ttccaaagga aggttcaggc
    13141 tgggacttag aagcaaatgc cttagttaaa aaagataata taaaaaaaga agcaaagcta
    13201 tttttagatt gggcaataag tgattcagct atggatagat atgcacatga agtagcagtt
    13261 acttccataa agacagataa tccagttcca aaaggttatt ctaaacaacc atttgaacag
    13321 cttataaaag aagttgattt aaactctgct gccaaaaaca gaagtacagt acttaaaact
    13381 tgggaatcaa aatacggttc aaaatctgaa agtaagtaaa gtttaaatta ataatttaga
    13441 tgaaagaatt ttagaagaaa aagtaggata taaaatttaa aataaaaaaa taactatata
    13501 aggagagata agatatgagt tacttaaaaa taaataatgt gtttaaaagt tatgaccaaa
    13561 aaagggtttt aaataatatt agtttggata ttgaagaagg agaatttcta tgtttactcg
    13621 gacctagtgg atgtggtaaa actacattac ttagaataat agcaggtctt gaagatgtaa
    13681 atagcggaac tataattctc caggataaag atattacaaa tttagagcca tcaaagagag
    13741 gttttggtat agtatttcaa tcatatgcat tatttccgaa tatgactgct tataacaata
    13801 tagcttttcc actaaaagag agaaaagttt caaaaggaaa aatagataat aaagttaagg
    13861 aagtacttga aacagttgga ttgactaatg aagctcataa atatcctaaa gcattatcag
    13921 gtgggcaaca acagaggatt gcaatagcaa gagcattagc tcttgaacca aagtttttac
    13981 ttttagatga accaatgtcc gcattagatg caaaggttag acacaagctt agaatggaca
    14041 ttaaaagatt acaaaaagaa cttaatataa cgacaataat ggtaacacat gaccaagaag
    14101 aagctataac tatggcagat aaaatagcta ttctaaatgg aggagatata atgcaaatag
    14161 ggactccaga agaaatatac caaaatcctc aaaacctatt tacagctcaa tttataggag
    14221 atacaaactg ttttgataat ggagattcta tattaactgt aagaccagaa tatgttcaaa
    14281 tagagaaatc gacaaaagaa aactaccaag gaataatatc aaatatagag tttagaggga
    14341 atttattgag agttgaaata aaagataaat taaatgaaaa tttcataata agtgatgctt
    14401 ctataaaaga gtgggttaat ttaaatttag tagaaggtga ccttgtaaaa ataagtatag
    14461 acgaaaaata ctatttaaag tatccaaaag ttaaaggagc ataagtgtat gaataataat
    14521 gctataagtt ctaaaagtat aagtcttaaa tcaaaaatga atttagatat aaaaaaatgt
    14581 atgcttacag tattagttat gtctttagta atatttttat tagcacctat aatatatcta
    14641 tttgtacagg cttttttaga taaagatgga gtctttgtag gattacaaaa ctttcaagaa
    14701 tacttggctt ctgaatctct attaaattct ctaaaaaata cagtatttgt atcagttata
    14761 tcaactgtaa tatcactaac acttgcatta gtatatgcat atgcattaac tagaacaaag
    14821 attaaattta aaggtttttt taaatcatta gctatgtttc ctatgtttgc tccaacaatg
    14881 atgcatgcaa taggtctagt atatttattt ggaaatcaag gtttggtaac taatttactt
    14941 ggagtgcatg ttccaatata tggagcagtg ggaataatat tggcacaaat tatatatgta
    15001 tttcctcaat catttcaaat actttatata tctctaagtt caaccgatta tagactatat
    15061 gaagctagta atactatggg gataagcaag ataagagaat ttataagcat aacaatacca
    15121 agtataaaat actctttaat aagttctgca ttcgtatcgt ttacattatg ttttacagat
    15181 tttggagctc caaaggtgat aggaggagag tttaatgtat tatcaataga tgtatataag
    15241 caaattatag ggcaacagaa tatgccaatg ggagcaactg tatcaatatt acttttaata
    15301 cctgcaataa tatcgtttat agtaactaaa aaaattgaaa aaaatcaaaa ttcatatata
    15361 acttcaaagt caattccata taaggttaaa actaataagt taagagatag tattttagga
    15421 ggggttgtag gaattattgc atttggtata atagctgtaa tgttaactgt aatcttatct
    15481 tcattaatat cagtttggcc atatgattta agactgacac tggaacattt ttctatgaaa
    15541 aacagtattg atgggattaa accatttata aattcaataa aaatgtcact attgacagct
    15601 attctaggga cgagctttgt tttcttattt gcatatttga atgaaaaaac agaaaaagct
    15661 ccatatttaa aatcatttgg gtatttttta agtacattgc ctatggcatt gccaggatta
    15721 gttttaggta tagcttatgt attatttttt aataaattag agtttaattt tatggataca
    15781 atatacatta agaatttgtt taatggatta tatggaacat tacttataat ggttatatgt
    15841 aatatagttc atttcttttc agtagcattt ataacagcta ctactgctat taagaaagta
    15901 gataaagaat tggatatggt agctaaatca atgggaataa acagcgcatc aattcttaag
    15961 aacgtaacta taccactttg tttgccagca gttttagaga actttatgta ctttttccta
    16021 aactccatgg tgacaatatc agcattagtt tttatatata ctgcaagttc aaaagtagct
    16081 gcaatatcaa ttgtacaact agatgataag gggagtactg cacaggcagc atcactagct
    16141 gtgttaatat tagtcacaaa tatattagtg aaagtaatat atgaatttat aaagaatcgt
    16201 ttagaaaaaa gaacaaactt taattaaata atttatctta taaaaacaca aatttaaaaa
    16261 agtctattct tgtgatttat tatttaaaga atagactttt taaatttatt attgatattg
    16321 tttattaatt ttccttttgt tatcatacat ttgagaatct gcacgacgat atgtatcaaa
    16381 taaagaaaca tctacttttt catcaaaaat agcatatcca atagacattg ataattttaa
    16441 ttcattagtt gagttttctt caactatttt taaattaata ttatttataa tattatttat
    16501 ttctttgtga gataaatttt ttagaataat tatgtattca tcacctccaa tacgataaac
    16561 tatatatttt ttatttatta catattgtat aattttactt gcttgcttaa tatataagtc
    16621 tcctgtctga tggccaaatt tatcattaat cttttttaaa ccgtctaaat ctattgatat
    16681 gatagatatt agattcttta gatttgaatt gtttaagtta ttcatatcta tttcaaaagc
    16741 atttctattt tttaggcctg ttaaataatc tgtattagca aaatcagtat atgtaggatt
    16801 agatattctt ttaaatagaa tcactgcaat caatgctgat attagacaag ttaataatgc
    16861 tattattggt gtaactatac ttataaattt aaatgtattg tattgatgtt ttgcttcaaa
    16921 ttcaattcct attactccaa caaccttacc aacatcattg tgaataggaa aatacgatat
    16981 aaatatgtat ccccaagatg ttgatttaat ctttttaggt aacaatatat gattatctaa
    17041 tgctttgtta aggtctcccc aaatctcttt ttctatcaaa tcaccagcat ttctaaaatc
    17101 actcctagat gaatttaaac catcaacaac atatactagt gatccatctt ttgttttttt
    17161 agcagtatat agatacatga ctccagtagt attttttaca gattctaaaa ggattttaga
    17221 acttttataa gacttttttg attggtcttc tttagaatta atttcagtaa aggtattttt
    17281 atcaatattt ctttctacat attcatagat atgttttgac ctctcactta aagagtatat
    17341 catgctctta taagttagtg tataattaaa aaaatataca catgaggaag atattattac
    17401 aataattgca gtaagtattg aaacctgcat atcaactcta ttgaattttt tatttatttt
    17461 catagcttaa atgtcttgtg aaaagaacct cccacaatct ccccataatt aatatatatc
    17521 tataaatttc accataatat attcatatat tatcaaattt gtgtggaaat tacaattagt
    17581 aaacatgctt aaagcattca aatatagt
//
LOCUS       DAEOSP010000062        17326 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.062, whole genome shotgun sequence.
ACCESSION   DAEOSP010000062 DAEOSP010000000
VERSION     DAEOSP010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17326
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.062"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            339..2426
                     /locus_tag="KRH54_001747"
     CDS             339..2426
                     /locus_tag="KRH54_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG8579412.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPKLEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            2441..2728
                     /locus_tag="KRH54_001748"
     CDS             2441..2728
                     /locus_tag="KRH54_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8579413.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            2755..4026
                     /locus_tag="KRH54_001749"
     CDS             2755..4026
                     /locus_tag="KRH54_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBG8579414.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            4084..4683
                     /locus_tag="KRH54_001750"
     CDS             4084..4683
                     /locus_tag="KRH54_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG8579415.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            4688..5593
                     /gene="dapA"
                     /locus_tag="KRH54_001751"
     CDS             4688..5593
                     /gene="dapA"
                     /locus_tag="KRH54_001751"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG8579416.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            5699..6331
                     /locus_tag="KRH54_001752"
     CDS             5699..6331
                     /locus_tag="KRH54_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBG8579417.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGETLCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNAKKIREIIKNVKKI"
     gene            6809..9316
                     /locus_tag="KRH54_001753"
     CDS             6809..9316
                     /locus_tag="KRH54_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG8579418.1"
                     /translation="MKEDIGLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDTYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNIVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSMI"
     gene            9658..11820
                     /locus_tag="KRH54_001754"
     CDS             9658..11820
                     /locus_tag="KRH54_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBG8579419.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKRLNKNYSKWIKDIADENNVTISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILKELNVDRSIFIARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYMLSLNGKKSYGFVNIMHFTLKIVFISFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSATARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            11825..12442
                     /locus_tag="KRH54_001755"
     CDS             11825..12442
                     /locus_tag="KRH54_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8579420.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            12631..12975
                     /locus_tag="KRH54_001756"
     CDS             12631..12975
                     /locus_tag="KRH54_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBG8579421.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            13545..14411
                     /locus_tag="KRH54_001757"
     CDS             13545..14411
                     /locus_tag="KRH54_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG8579422.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            14524..14928
                     /locus_tag="KRH54_001758"
     CDS             14524..14928
                     /locus_tag="KRH54_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBG8579423.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            15092..15541
                     /locus_tag="KRH54_001759"
     CDS             15092..15541
                     /locus_tag="KRH54_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8579424.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(15697..16074)
                     /locus_tag="KRH54_001760"
     CDS             complement(15697..16074)
                     /locus_tag="KRH54_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG8579425.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            16336..17253
                     /locus_tag="KRH54_001761"
     CDS             16336..17253
                     /locus_tag="KRH54_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBG8579426.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFRNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
BASE COUNT         6785 a         1864 c         2932 g         5745 t
ORIGIN      
        1 tttaatatat tagaattata aataaggcac attaatttta ttttaaatta atgtgccttt
       61 tatttatggt aatttatgca taattagtat cgttttgatt ctttaaataa atttttcttg
      121 gtttcttcac ataaggaact ttttttaatt tataaaatct acagttatat tatagtatct
      181 attttagaga agtttaattt ttgtatcaat ctaaaacaaa agttatactt aaatgaaatt
      241 gaaaacgata aaattctcaa ataaaataag tattgtggag gtgatgaaag aatccaatga
      301 taatatgaag actaatctta ataagaaagt aggggttaat ggtagtggac aaagaaattg
      361 tatcaattct ccagatttta ttaaatagca cattgattac aactaatgga ttacaagagg
      421 agtctaattc atcaaagaga caaattactt atagaataaa taaaatcaat gacatgctta
      481 aagtaaaaaa tgtaccactt atatgtttaa gagctgataa agatattatc gttagaaaag
      541 aaacaaaaaa agcaattaaa gaaattttag aaaaaaacta ttctaagaat acttattatt
      601 ttagtaagac agaaagactt ttatatatgt atttaatgct ttttattaac atggaggata
      661 tttcaattaa tcattttata aattctataa aagtaagtag gtctactgta aatcttgatt
      721 tcaaggattt gataccaaag cttgaaaaga agaacattaa agttaagaac aatcgtataa
      781 atggatatta catagttggc aatgagatgg aaattaggag agtgcttatt aaaaatatta
      841 tagaaacttt gtctaatgag aaaagttcaa gggtatttga tatttttatt aaagaatata
      901 atttagatag ttttgaagag gctaaaaaaa ttatattaaa gctaataaaa aaatatgaaa
      961 ttacttttgt tgaagataga ttaatagaat ttatatatat atttatcttt ctaaaggcaa
     1021 ggatgtattc aactagaata gttactcagg aaaatgtaga tattcctaat atttcagtta
     1081 tgagttcaat gaaggagtat aaatttgcta aggaattatt agaagtatat gggagtgaag
     1141 ataaaataaa gcctcacaat ataatgtata ttagtgcttg gattttaggt atatcagtag
     1201 gaaattatga agaagataca gaagataagg atgtaatttc aaaaattgta aataaaatga
     1261 tgacaaagtt tggatattta agtggagttt actatataag ccaagaaaaa atatttaaac
     1321 aattatattc tcattttaga cctgcctact atagactttt atttaaatta cctatctata
     1381 atccgatttg tgaaaaagta aaagaagaat ataaattagt ttatagaatt gtaagtaatg
     1441 caatgaaaga gttttgcgga ttatttggag aagaaattca ggaagaagag ttagcatatc
     1501 ttacaatgca ctttgcaaca cttttttcaa ataaaaaaga atttgatggt tttagaaaaa
     1561 gaatagcatt aatagtttgt tcaaatggcg ttggtagttc agctatttta catgcagaac
     1621 ttacaaactt atttcctgac ttacacttta taccaatgtc ggaatcagtt ggattagaaa
     1681 atataagtga acctgtagat attgtattta ctactaatta taacgcagag ggtttaaaaa
     1741 cagatgtacc agtaattaaa gtaagccctg ttatgaccac aaaagaaaaa tataagatta
     1801 cacgtgaagt gtatattcaa ttaggtgata tttttttaag acaacctaaa atagatgagg
     1861 ttatggatat aataaaaaaa tatgcaaatg taacctctga aagtattctt tcaagtgagc
     1921 tattagctta ttttacacag atagagaatt ttacatctaa ggaaggagag gggcctatgc
     1981 taagtgagat tactaatgaa acactagtaa aaataaaggt aaaagctaag aattgggagg
     2041 aagctattag acagtcagct tctgtattag ttgaaaataa caaagtaact gaagggtatg
     2101 tagatgctat gattaactca gcaaaagcat ctggaccata catagtaatt acaaaacatg
     2161 ttgcacttcc acatgctaga ccagaagctg ggtcaaaaga gattgcaata ggcattgcta
     2221 ctttagatag tcctattgag tttgggaata acgaaaacga ccctgtaaaa tatgtatttt
     2281 gtctaagtgc aatagacaat aatagccatt tacgtgctat gtctgagcta gttgaactat
     2341 tggaagaaga tgagtttttt aaggttttag atagtgctaa agaagctaaa gaaattattg
     2401 attttataaa aacacacgaa ttataaagga ggatttaatt atgtataaaa ttttaatcgc
     2461 atgtagagca ggagttggct caagtttaat gttaaaaata aagactcaac aaattattaa
     2521 ggaaaataac tttccaattg aagtagaaca tggttcatta gattcactaa atggattcaa
     2581 tggagatgct gtagaaacat taattgatgt tgctgaagaa ttaaaaaata aaaatttgaa
     2641 gtttgatata attgggattc acaatattgt tgaccgtaat gaaataaaga cagcactaga
     2701 aaaattcctt gcttcaaaag agggataaat ttaagatgat aaggaggaat atttatggag
     2761 tttattatta gtttgctgag tacacctgct gttttgcttg gcgtagttgc atgtatagga
     2821 ttgattcttc aaaagaaatc agctgttgaa gtatttacag gctcttcaaa aacacttatc
     2881 ggttttttga tatttggtat tggtgcctct gctatgacag gagcacttca aaatttcaat
     2941 aaactgttcc aacatggatt tggaattact ggtgttatag cttcaccaga agcagctaca
     3001 gcacttgcac aaggtacata tggttttgca gtttcatgta cattaattct tggattcata
     3061 ttaaatttag tatttgcacg tattacaaag tttaagaata ttttctttac aacaggtcat
     3121 agtttgttct tttcttgtgt attagtttta attatgaaag cacatggatt tgataataca
     3181 atcactattt taataggagg aactatctta gggtttatgt ctgctgcact acctcaattc
     3241 tgtcaaccat ttatgcgtga attaacaggc ggcgacgaac aagctattgg tcactttaac
     3301 atgattggtt atggtttatc aggttatatt gggcgtttat tttcaaaaca taaagatgat
     3361 acaacagaga ctatagaatt tcctaagtgg ttatcaatat tcagagactt cttaatgggt
     3421 ctatccatag ttatgttaat cttattttat attgcaacat taaaagctgg tcaagcattt
     3481 actcaagaaa ttgctggttc aacacattgg ttggtttttc cgcttataga ggcatttaca
     3541 tttgtggcag gtatgtcaat attgatgtca ggtgttcgta tgttcttagc tgaaataact
     3601 gcagcatttg taatcatttc agaaaagtat attccaggtt cacgtcctgc gctagatgtt
     3661 ccaaccgtat ttccatatgc accaaatgca gttattattg ggtttatatc agcatatgct
     3721 gctggtctat tagcaattgc tattatggca tcatttccta gtatatttcc tgtagtaatt
     3781 attccagctg ctcacatatg ttttttctca ggaggtacag ctgcaatatt tggcaatact
     3841 tcaggaggat ggagaggggc tattgctggg tcatttgttg tggggttatt acttgcattt
     3901 ttgccagtta tactatatcc agtttatggg actcttggta tagaaggagc aactttccct
     3961 aatattgatt acaacgtagt tggttcgata ttacataata ttttacaatt tataaaacct
     4021 atttaaacta aattatataa aagtatagtc atagttacta tgatttcaag gaggatacat
     4081 attatggaaa atacattatc tgaattttta gataatatat ctaatgaatt gtcagaattt
     4141 attgcctcaa tagatgagac atctataaat aaagcatgcg aattaattct tgaagctgaa
     4201 aaaaatcacg gacgtgtaca tgtgactggt attggcaaac caggacatgt atcgggatat
     4261 atttcatcat tgctttcatc tactggaacg tcagcatata tattgcatgg tacagaagca
     4321 gtacatgggt caagtggtca agtagttgaa ggagatgttg ttattgcaat atcaaatagt
     4381 ggtgaaactc aagagttaaa agcaactctt aagactctaa aagtaaatgg agcaaagata
     4441 attggtgtaa gtggtaatga gagttcattt ttaaaaaata tttctgatat attcctgttt
     4501 gcaggtgtta aacaggaagg agattgtctc aataaagctc ctcgtgcttc aattttagct
     4561 gaaacaataa tattacaatc attaagtgtt gtattacaat atgctaaagg tcttaataca
     4621 cagcagtatt tgaagtggca tccagcagga agtcttggaa agtctattag agagggaata
     4681 taattttatg aaatataaag ggattatatg tgcaatgata acaccatttg atgaaaatca
     4741 aaatattaat tcacaagcaa catgtcaatt aattgattat ttgattgaaa agggaattta
     4801 tggattattt attttgggca ctaatggtga atgtcatgta ttaacagatg atgaaaaagt
     4861 tgaatttgca aagattgtta ttaatcatac aaataatcgt gtacctgttt ttgttggaac
     4921 agggggtaat tcaactcgtg aagttgtaga tttatctaag agaatggaag cgattggagc
     4981 aagtgcatta tctattatta caccatactt tgttacacca acacaacagg aattaatctt
     5041 acattacaaa actattgcta attcagttaa tttacctatt ataatgtata atatgccagg
     5101 taagactgga attaatattg aaccagagtc agtacgtgaa ttatcaaaga taaagaatat
     5161 tgttggaatt aaggatagta gtggtaaact tgacaacatg aaagcttata ttgaagcaac
     5221 gaagaatgag aattttagtg tgttttcagg ctcagattca cttattctgg atagtctaaa
     5281 agctggtagt caaggagctg ttgcagcaac aacaaacttc ctcacagaga ttggtatctc
     5341 tatctatgaa aactttatga aaggggattt ggaagaagct caaaaagctc aaagtagtat
     5401 agaagaactt cgacgtatac tgaaacttgg aacaattcca tctgttatga agaaaactat
     5461 tgtcttgaat ggaattaatg ttggaactgc acgacttcct gtaactgaac caactgggga
     5521 agtacttgaa gaaattaaac gagtagttga aaattatagg acaatactta ataaaagtag
     5581 tgagaataga taatataaat agggaaatta cactggtata taattaatat atatattata
     5641 aaacatgact aaaattaatg aataagtaaa gtggtttgat gagtatagag gtgataatat
     5701 gaaaaagatg aatataacca aaagaatgtc agaatgtggg gctcttgcaa ttgtaagaga
     5761 agaaaatctt aaccgtgcat gtgaaattgc tgaaggatgt ataaaaggtg gtataacagt
     5821 tatagaaatg agttataccc taaataatgc tggagaaata attcaagggc ttaataaaaa
     5881 gtatggtgaa acactttgtg tgggagcagg aacagttttg gatagtgaaa cagctcgtca
     5941 tgctatatta catggagctc aatttattat tgcaccaaat tacaatgaag gtgtagcgaa
     6001 gatttgcaat agatatcaga ttccttatgc tccaggatgt acatcattga ctgaggcagt
     6061 agatgcacta agtttaggag caacttttat aaaagctttc ccaatatcag atttctatgg
     6121 acctaaacta gtaaaggtat ttaagacacc aatacctgat atgccagttt tagcaagtgg
     6181 agggattaat attgaaaacc tacatatatg gttagaaaat ggagttgatg tatgtggatt
     6241 tggtggttta ttaactaaag gttctgttga agatattgct aaaaatgcta aaaagattcg
     6301 tgaaatcatt aaaaatgtaa aaaaaattta acatagggct gtatcatagt atcttataat
     6361 tactatgata cagccttttt aatattattc aaatttaaaa cagtttacat ttctattata
     6421 ttacatataa tttcagtaat tttatattaa tttttctatc tggaaaaaaa tgatttaaaa
     6481 cttaagttat gatataattt cattaaaaga aggcgatttt tagtaaatat gagaaaataa
     6541 tgtgttttta ggggaaaagt taaatacaga tttaagtgaa caagaatcga taattaagat
     6601 attaaatggt gaatagggca tcaaaaaaat tactaaatta aaagttttag agggagaatc
     6661 ttagcaagtg tgtgtgaaaa ttcaatttca aacaaaaaat aattgtaaat gaaatcaatt
     6721 tatacacgtt gaagattcac ttttatcatt atattaaaaa gcagagtgaa ttaaaagtga
     6781 gtctaaagat aggaggataa ttaaaacaat gaaagaagac attgggttac atcgaatgtg
     6841 taaaaaagaa caagcaacaa agaatgaagt tatatcagag gatattctaa aatctatgag
     6901 tattggggtc agtaaacatt ttttagatga ttcatttaca ataatatggg gaaatacatc
     6961 attctatcaa ttattaggat atacagaaga agaattttta gttaaatttt ctagtttaaa
     7021 agattattat gaacagaatc tttatcattt tgaatctatg aaaaatcatt ttttagaggc
     7081 ttatcatcat ggcgaaaaga gtatagaata tcaaatttgc atttcagcaa ataatggaga
     7141 ccctgtttgg gtaataatga ctggtatatt aattgaatca caagataatg agaaatcaat
     7201 ggtctatata ttgtattcaa aaattaatga gttaaaaata aagcaagaag aactatctga
     7261 tatggaaaaa gagagtacag agaattttaa atggatgatg tcagagtatg ctgggaatgt
     7321 ttatattagc gatatggata catatgaatt actatactta aataagcact catgcaatac
     7381 tttacaggca tcagcaaatg aacttattgg aagaaaatgt tatgaggtaa ttcaaggtag
     7441 aacttcgcca tgtccgtttt gtacaaattc gtatttaaaa aaagatgaca catatgaatg
     7501 ggaattttat aataaaaaac tgaaacgaac atttataatt aaagaccgta tgttgaattg
     7561 gcaagggcgg agagttcgta ttgaattatc ttatgatatg tatagtgctg aatataaatt
     7621 agcaaaaaaa gatcaggaaa gagaatcaat tttgaaaact attccagcag gaatggttag
     7681 attagatgca agagattatc ttactatact ttggtgtaat gatatatttt tgaatatgat
     7741 tgaatataca aaggaacagt ttgcagagga acttaataat caatgtggat atttattttc
     7801 tgaagattat aaacgtagta agaaactggc acaagatttg aaggagtctg gggataatat
     7861 tgtatttgaa gcaaaaatat atacacgttc aagggaagaa cgtatatgga ctgttacatt
     7921 gtgttatatc agtggggaag acagttggga tggaatacca tctttttata gtataggtat
     7981 tgacataaca aaggaaagaa aacagattga gaaattacag catatatcag agaaagatgc
     8041 attaacagga atttataatc gtgctgagac tgaaaggcag attaagaaat attttgaaaa
     8101 aaatttaaat gttatgggtg cactgtttat gattgataca gataatttta aacagattaa
     8161 tgatacagaa gggcatatga ttggagatat agtacttact gagatggcat caggtatgaa
     8221 aaaaataatg cgtgacagtg atgttgttgg aagaataggt ggagatgagt ttacaatttt
     8281 tatgaagaat atttcctcag ttaaagatgc agaaaagaaa gcagaagaac tattgtatat
     8341 gttccgtcat ctatttcaga aagaaaaaag ttttctaaaa gtaacttgta gcataggaat
     8401 agctatatat ccaaaggatg gtactacatt caaggaaata tatgcaagag ctgataaagc
     8461 cctgtatcaa gcaaaaaata tgggaaaaaa taattatgta atctacaacc aagattattt
     8521 taaagaatta gaagaatttg attgttcttc tcttggtaca gttattgatt cagaaaaaca
     8581 atacgctgaa tatcctgata atcttacacg ttatatattc cgtatgcttt atcagactga
     8641 tgatatagac caggcgatta atatgatact tgaggttgtt gggaaacagt ttgatgtaag
     8701 tcgtgtttat atttttgaaa atacagaaga tggtagatat acttctaata cttatgaatg
     8761 gtgtaatgaa ggaatctctt ctcaaatggc ttatttgcaa aacagcaatt atcaagacta
     8821 tgaggattat gaaaagcttt ttgaggatga attagttttt tattgtcgag atatccatac
     8881 attaagtcct aaactagaag aattgttttc tagtcaaggt atccactcta cacttcaatg
     8941 tgcatataaa gaagatagag ttttcagtgg atttgttgga tttgatgaat gcacaggact
     9001 acgtctatgg tcacaagagg aaataagtac attatcattg atttctcaaa ttatatcaat
     9061 gtttttacaa cgtaaaaaaa ataataaact taatcaacag attcaacaat accagaatat
     9121 tttagataat attgaccagt gtatttgtgt gattaataaa gaaaataatt ttttattata
     9181 tgtaaataaa aaatttaaaa atacatattc taaattccaa attggacaac cttattatct
     9241 tgattctata gatattcata agatatccat tatatggaat aaaaaagaag catacctatg
     9301 tactagcatg atttaatggt aaagtaaagt ttatattgga cttaaattaa attttgtttt
     9361 aaataagata ttattttagt tgattactga aaagactaga atctatatta atgaattata
     9421 ctaattttgt accactatta aaatattgtt tgctaaaaat aaaaaatagc cttaaaacag
     9481 ttgattataa aaaattaact gttttaaggc tatttttatc tttgtgacga aatagacact
     9541 tgccatttag aatgtgacaa tatagtagtt gtttagaaaa ggcacaaagc attatacttt
     9601 aactaaagta aaatgtattt tgcttttaga ataaaaacaa ttaggaggga gctataattg
     9661 aaaaagataa tatatgtatt atttacaatt gaactattaa tagtatcttt attaggtcta
     9721 aatatagtta aagataatga aattaataat attttatata acgatacaac aagtatatct
     9781 gtaatgttta aagattataa aaggttaaat aaaaattata gtaagtggat taaagatatt
     9841 gcagatgaaa ataatgtgac tatatcaaag tatgttttta ataataatga aaaattatct
     9901 atatatacga ctgatacaag tttgaataac aatataaagt taaaatcagg aaaatttcct
     9961 aatactaatt caaatgaatt tattagtaat ataaacacta ataatagtaa tcaagtaggt
    10021 aaattctcaa ctatgagtaa agacatatca attttaatta gggattttgg taaaataagt
    10081 gaagttggag aatcaggaat cttatatatt tcaactcaaa atgaagatac aataaataaa
    10141 atattaaaag agttaaatgt ggacagaagt atttttatag caagattaca tgatagatat
    10201 gttaactcaa atttatactt aaatccttca ataattagag atttaatact aattatcctt
    10261 tgtttcttgg ctacaataat acattattca attagtgtga gtagagaggc ttctatttta
    10321 agattaaatg gatactctaa gctagatata attgctagag ttataaaaaa tatacttaga
    10381 attatgattt tatcatctgt cacagcatta ttaatatata taatctatat tttaaaatta
    10441 aatataggtg ttagagcatg cttatacttt gctatgtttt cgattatatg tattttgata
    10501 aatattttga tatctatatt aatagttggt tttaatagta gaagttctaa atatatgcta
    10561 tctctaaatg gaaaaaaatc ttatggtttt gtaaatataa tgcatttcac attgaaaatt
    10621 gtatttatat catttcttat attatccata aataactgta ttttaaatta taatatgcta
    10681 caaactcagt taggaaatct atctgagtgg gataaagcaa aaaatgttta taatcttact
    10741 cttaaagata caggtgaaca atctttagga aaggaagaag taattaaaaa tgccaagatt
    10801 aaaggtttct ataaaaattt ggtagaagaa aaagatgctt tcttaataga ttcaactaat
    10861 tttgaaaaac ttgaagatgg aagttacatg tatgaaataa attcaaaggg taaaaaccca
    10921 gaagtaagtc aatatggtaa aaatataaaa ataaataaaa actatcttaa agttaaccct
    10981 atttactcaa atggcaaaga aatatttaat ctaatcgatt atgacgataa gactataaat
    11041 ttattagttc ctaaaaagtt acaagtacat gaaaatgaga ttaaaaaaga gtttagagaa
    11101 ttattttatt ttgaaaaaat agaagtagaa aatatgtaca ataaagagtt aaatagagag
    11161 ttaaataaaa caaagaagtc agatttaaat gttaatataa tatatgtaga taataatcaa
    11221 agctatttta cttataactc ccttgttatg gatgacaata ggaatctcat tgatgaccca
    11281 atagcaatag ttgacataga taatattgat gattcttttt atctttctta tatatctaga
    11341 tgtgtatatt ttaattctaa gaaattagat gcccttgcag atataagtaa cattattgaa
    11401 gctcaaggtg tagaagcgca tatccaaagt ttacatgctg tttataatga gtatggttta
    11461 gaaataaata aactagaaaa atatttgaat agtgaaattt ttactataat aataatagca
    11521 atatcaaact taatgattac ctacaatata gtagctagct actatgaaag aaataaatat
    11581 aagttatata ttaaaaaaat atttggttat tcagctacag ctagaagtat gttgttaata
    11641 gtctcattaa tattaacaaa cattattcca ataggcatta tatcaacaag agtagattta
    11701 agtaataata ttatcttatt tggacttcta atattagtta tagaagtaat tgtatctatt
    11761 gtattagaca agattatctc aaatagttca tttaataaaa ttataaaagg agaacattaa
    11821 aaagatgatt tcattaataa atatatctaa aaaatttaaa gataaatcta taatagacaa
    11881 atttaaccta gaaatttcta agggcgagtt tgtagcgata acaggaaata gtggttcagg
    11941 taagacaact ttacttaata taatgggatt attagaaaaa cctaattctg gtgatattat
    12001 aataaataat ataaaaaatc caaatagtaa acagataaga attttacaaa gggattttta
    12061 tggctatcta tttcaaaatt atgctttaat tgagaatgaa aatgttgaaa ataacttgaa
    12121 aattgcttta aagtatcaaa aaaatatcaa ccataatgaa aaaataaatt acgcacttga
    12181 gtctgtaggt cttagaaaat acaataaaaa aaagatatac gaattgagtg gaggagaaca
    12241 acaaagggta gcattagcaa gagtaatatt aaaagaatgt gaagtaatat ttgcagatga
    12301 acctacaggt aatttagata aaaaaaatag agacatagta tttgatatac ttaaaagtct
    12361 aaataagaat ggtaaaacta tcgtatttgt aactcatgat ttagagttat cggagcaggc
    12421 agatagagtt ataaaattgt aataatatca actataataa atataagaaa gagattacag
    12481 ctaaatcaag tttaataata atacgaatag aatttaaaca ttaagtaaat tagtagtttg
    12541 taaatataag tataatatga tatcataaag gctcaaaaga aattttatat aataagaaat
    12601 ctaaaaataa caaatggagg acatataaag atggaaaatt taccaaattg cccgaaatgt
    12661 aattcagaat atacttatga agatggaact cttttagttt gtccagaatg ctctcatgag
    12721 tgggcattag gattgggagc tgaagaagat tcaaatatta tcaaagatgt aaatggaaat
    12781 gtattaaatg atggagattc tgttacagta attagagacc ttaaagtaaa aggaagttca
    12841 tcatctataa agattggaac aaaagtgaaa aatatccgtt taatccatga ctcatcagat
    12901 ggacatgata tagaatgtaa gatagatggt tttggagcta tgaagctaaa gtcttctgtt
    12961 gttaaaaaag cgtaatatta taaataagct gtagtgttta ttgtttttca gtacagtata
    13021 aaaccataag aatctattaa gtaataaaag tttgtttatt tatctttata ttttcttatg
    13081 gttttattaa atataaattt tattgaacac caaaatattc tctcctctac aaataagcca
    13141 atcttattta aactaatata ctttgtacta tttatactga aattgtaaat ttaaatgcta
    13201 tgttatttag gtttgtatag ttaatttata tatttctgat tttataaatt gtatttatta
    13261 ttatatcttg attctaaaat cattaaaaat ttcaacttct ataaagattt acaatttatc
    13321 aaatacatta tgagtatata aacaatttat tattacagtg ctacatttaa aataagtctt
    13381 agtataaagg tattgttaca agtataaaga aacttatgta tgctattgga ataaataata
    13441 ccttaaatta taattaagaa taagaactgt taaataaggg acttgattat catttagtta
    13501 tagataatat aatattaaaa ataaagttta agaggagaaa aaatatgata gatatacata
    13561 aaaatatata tgataataag ttatttgagg agctaaagat tgactgtaag aagtgctttg
    13621 gactttgctg tgtagctctt tatttttctg cttcagatgg atttcctatc gataaggaat
    13681 caggaaaacc ttgtattaat ttacaaccag attttaaatg ttctgtacat aatagtctta
    13741 tgaagcgagg ttttaaaggg tgtactgcat atgattgttt tggttcaggt caaaaagtag
    13801 cacaagtaac atataagggg atagactgga tgcaatcacc agaactaaca aatcaaatgt
    13861 ctgaagtgtt tttaattatg agacaattac atgaaatgtt atggtatctt aaagaagcat
    13921 ctgtattaaa tatatcagat acaatcaaat caaaaattga tttaataatt gaagaaactg
    13981 aaaagataac taatatgggc ccagaacaga taattaattt ggatatcata tcacatagaa
    14041 caaaggttaa tttattatta tcacaagcga gtgagtctgt gatgggaaag gttaaatcat
    14101 ttatcaagac ttctactctt aaaaacatga aaaaattaag taaaaatata gatttaatag
    14161 gagcagacct tagaaaaata aatctaatag gagcagattt aagaggtaga tttctaatag
    14221 cagcaaattt aagaaataca gatttgagtg gggcaaattt aataggagca gacttaagag
    14281 attgtgatat tagaggtgct aatcttgaaa atagtatatt tttaacacaa ttacaagtaa
    14341 atactgcaaa aggagattct agtacgaagt taccagcttc actaatacgc cctaaatact
    14401 gggaaaaata attaaattaa tacataattt tacaaaatat gaactttttt gactttaatt
    14461 catattatac attagagatt ctttacaaga atttcttaat ataatatagg gagagatata
    14521 aaaatgagtt atcaaaatta taaatgtgaa agagattatg tgtatgatga ttataaatac
    14581 aataagtgca acaagtgcta taatgattat gaggaaatga cacatgttca tgaatattca
    14641 gaaagtgtta agttagcaga agaatgtgag gatagacata accaccgtgc agcaggggtt
    14701 actggtgaag ctataccaat aaatggaggt acaaatcatg ttcataaaat aaatgataat
    14761 gtagatttcc ttgaccattt ccataagata tgtgttacaa cagggccagc aattagaata
    14821 cctggaactg ataaacacat tcacttaata tgtggagaaa ctactgttaa tgatggtcat
    14881 tgtcataaat tcctttttac tactcaaata gaagcacctt tagtataagc tgatgtgaat
    14941 tattgtagtg aatttgctag attatagtat aatatataat agataatatt taaacttaat
    15001 gaggactatg atatttaacc ttcgtagtcc tttctaagtt ttgtataaaa aagtgttatg
    15061 caattaaaag tgatataggg aggaaatttt tatgaaatct ggaataattc ttgaaatgaa
    15121 acaaataaat tcaacagaat atgacgaaat gttgcaagtc tgggaatcat cagtaagagc
    15181 aacgcatgat tttttaacag aaaaagatat tgaatctttg ataccattag ttgagattgg
    15241 acttaaagaa gtagaaaata tagtttgtat aaaagataat gatataataa agggctttat
    15301 aggaatagat aaagataaaa ttgagatgct ctttatagaa gataaatata gaggaaatgg
    15361 tattggtaaa aaattaataa aatatgctat agataaatac aatgtaaaat atgttgatgt
    15421 aaatgagcag aataaaaaag ctgtaggatt ttatattcac ttaggattta aagtgtttga
    15481 taggtctgaa atagatggac aaggtaaccc atttccaata ctacatatga aattaattta
    15541 aagttatact tctcatttat tcgcaaaaat atttacttaa atttttatac aaataaaagt
    15601 cgtgacagtc ttaggacacg acttttttac tacactatta aattgaaatt tatctgttct
    15661 atattatgat tattagatgt agttttttta ttttaactat gtacttaaat accaagtata
    15721 cccataccta tcaagtactt cagcagcgcc atcattttga tgtggtggtg ggtgtggtaa
    15781 aaattcttgt acaagtgtac caccttcggc aagttttaca gatatttctt ctatcgcttc
    15841 tcgagatggt agaacaacac aaatcgccat gccagaaatt acaacaggct tgtctgtatc
    15901 cgcacccatt atacaacctt catattttgt gaaagtaaca cttgcgtgca taataggatt
    15961 gtttggattg ctgtctaatc gcgcaaatat agtttttcct cctccgaaag cctcagcata
    16021 aaaattaaat gctttttcac aagtaccatt aaatattaaa tatggagtta gcatgaccaa
    16081 tatatcctcc ttgatataga ataatttata gttgcacctg ataatctaaa ttatgattta
    16141 gtaaattgta tcatagggaa accttgaagt atataatttt ttattattat attatacaaa
    16201 tttattaatt taaaaaaatg ttatatgtac ggaaggttta caggataaaa tataataaaa
    16261 agattaaggt gacattataa aaaatgtata ttgacattga cgctacgtaa ttgtatatac
    16321 tttgtcttaa aggagatggt aaatatgaat attatagaaa ttcgctcaga taaaatatac
    16381 aagaagataa tggatgcacc aataaacaaa aaagaagata tatacagata tgaattgatg
    16441 aagccttttg aatttaagtg gaagtgtatg aatgttccaa tagttgctag acagaaaggt
    16501 ggatatgatg taattatagc aagtgaaatg ttaggggttt tatcgcctaa ggatattgat
    16561 gaaaagcaaa aaaagaatat aaatgtgtta tctgctgata aaatttgggg cacttgcaaa
    16621 gaaaccatag aaaactctat aaatgctttt ataaaagaag ggtatgattt aaacattaag
    16681 gactataaat attcaatatt attggcgaat ccaaatagtc cttatacaat attaagtgat
    16741 ggatactggg gtgatggtgg gattcctgga tatatatttc tatcattggt tcctaatgaa
    16801 tatactatca atagattacc agtattaata gcacatgaat gtaatcacaa tattagattt
    16861 cagtttatag agtggaataa taatataaca ttagaagaaa tgatgataaa tgaaggtctt
    16921 gcagaaaatt ttgcaacatg gatgtttgga gaggaaatgt taggaccttg ggtcagtaga
    16981 acagatatcg aaacattaaa tacttatata aagccaataa taaaaagtgc tttaaaagaa
    17041 actggatttc gaaatataac atcttatctt tatggtgatg atatagctaa aatgcaagga
    17101 tattttccag tagggttgcc ttattgtgca ggatatgctt gtggatatta tatgattaag
    17161 tattatttag aaaagacaaa taaatcaata atcgaagcga ctttattgcc ttatagtgag
    17221 ataatcgaag cagtaaaaga gttttgggaa taaattttat aaattgcata atttgaaata
    17281 aattatgcaa ttattttatt tttaagaaaa aataaattga caataa
//
LOCUS       DAEOSP010000063        17286 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.063, whole genome shotgun sequence.
ACCESSION   DAEOSP010000063 DAEOSP010000000
VERSION     DAEOSP010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.063"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            370..2604
                     /locus_tag="KRH54_001762"
     CDS             370..2604
                     /locus_tag="KRH54_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG8579427.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIKLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2635..3468
                     /locus_tag="KRH54_001763"
     CDS             2635..3468
                     /locus_tag="KRH54_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG8579428.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKKKFDKND
                     Y"
     gene            3708..5441
                     /locus_tag="KRH54_001764"
     CDS             3708..5441
                     /locus_tag="KRH54_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG8579429.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENTQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDNTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5773..6127
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5902..5930
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(6657..6761)
                     /locus_tag="KRH54_001765"
     CDS             complement(6657..6761)
                     /locus_tag="KRH54_001765"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBG8579430.1"
                     /translation="MDDYIYYYNNFRYQNRLNSMSPFEFRKYLSTERA"
     gene            7386..7631
                     /locus_tag="KRH54_001766"
     CDS             7386..7631
                     /locus_tag="KRH54_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579431.1"
                     /translation="MNQKKIKISIKMVIIGIFAVIIAFVLSRLFVEYIDELLRSNWQY
                     QLFESESSYIVFANCIMFSNILLEIFLIYICRKFRKI"
     gene            8626..9275
                     /locus_tag="KRH54_001767"
                     /pseudo
     CDS             8626..9275
                     /locus_tag="KRH54_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
     gene            9816..11171
                     /locus_tag="KRH54_001768"
     CDS             9816..11171
                     /locus_tag="KRH54_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8579432.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(11289..11957)
                     /locus_tag="KRH54_001769"
     CDS             complement(11289..11957)
                     /locus_tag="KRH54_001769"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBG8579433.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(12024..12452)
                     /locus_tag="KRH54_001770"
     CDS             complement(12024..12452)
                     /locus_tag="KRH54_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG8579434.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            12559..13152
                     /locus_tag="KRH54_001771"
     CDS             12559..13152
                     /locus_tag="KRH54_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBG8579435.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            13271..14206
                     /locus_tag="KRH54_001772"
     CDS             13271..14206
                     /locus_tag="KRH54_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBG8579436.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(14478..15422)
                     /locus_tag="KRH54_001773"
     CDS             complement(14478..15422)
                     /locus_tag="KRH54_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG8579437.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKICYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            15740..17170
                     /locus_tag="KRH54_001774"
     CDS             15740..17170
                     /locus_tag="KRH54_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8579438.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
BASE COUNT         6651 a         2246 c         2198 g         6191 t
ORIGIN      
        1 aagttaatta tgaataaaat aattatgcct ttgaaaaatc attaaactta aaattaatta
       61 taaaaaaact ctaccttact aactatacag ctcacaatct atctatttat gaaaaaatat
      121 gatataattg cattcaaaaa ggaaatcttt caatatacta gaatttaagc attaactaat
      181 tttatctaat attattacta aactaaaaaa tatttgtatt tgtagcattg gagcaagtaa
      241 cttaatgtat attcatttta aatacttatt taaaatttag catttaaatt atttacaaat
      301 taacatttaa atacttctag cataaataca gttgtcatta aaatttaaag tatcttatgg
      361 aggaatacca tgcaaaatca taattatata aaaatgttaa ataaaaaggt agaagtatta
      421 tctaaggaat tagatagtag aaattttaaa ttaaaaacct taaatgaaat agattttttt
      481 tctaaaatac caaacagaga atttatgtac caacatattg atgactgtat ttcgaaaaat
      541 atacatatag gaatcatgtt attaaatatc gataacttta aattatttaa tgatatatat
      601 ggatacctag tcggagaaaa tttattaatt actctaactc aaaacctatt gcactttatt
      661 ccagaatcaa agggaatagt cgcttattta aaaggagaag gttttttaat tgtacttcca
      721 aatgtatcaa ataaagagtt aaataatatg ggtgcaacta ttatagagag cgcccgagaa
      781 ttaaaaataa aacttcctaa tgaaaaagaa atatactcca atgttacatt tagtatcggc
      841 agcactatct ggaatggtga accaaacatg actactttga tgcttttcaa ccaagtatat
      901 agtgctttaa aaaaggctaa aagtgaaggt aaaaatagat acattctatt tgattttaaa
      961 gataatagtt tttacaataa attagaaaat gataaggagt tcttatcaga aatatcacat
     1021 gttctacaaa ctaaacaaat tgaagtattt taccaaccac tttataatat aaaaaataat
     1081 actatagttg gatgtgaggc acttgcaaga tggagacacc ctattaaagg acttcttact
     1141 ccagataaat ttattccttt atttgaacag tttggtctga taacggaatt agatttgcat
     1201 ttatttgaag agaactgtaa aaatataaga agatggattg ataacaatga tgtttttgtt
     1261 ccaatttgtt gtaacttttc tcgtcttcat ttttttaacc ctaattttcc aagtatgcta
     1321 aaaggtatta ctgaaaagta cagcgtctct acatcaaatt ttagcattga aataacagaa
     1381 aatatattaa tagaaaatac aaatacaatt attcaacaac tagctgaact tcgttcttat
     1441 ggtttttctg tatcaatgga cgattttggt tctggatatt catcttttgg tatgttgcaa
     1501 aatctacctt ttgatataat caaaattgat aggatatttt tatctcataa tttaaatgat
     1561 tttaaaaata caaccatttt gtactctata ataaacattg caagagctct tgggatgctt
     1621 acagtatgtg aaggtgttga aacagaacaa catgtagaat ttattaaaag tataaattgt
     1681 gatatcgccc aaggttatta ttatgcaaaa cccatggagt gcaaagaatt tgaaaaatta
     1741 ctatctaaag agaataattc agagaaaatg tttataaaat attctgatat aggaaaatct
     1801 ttaatagaga agatatttga tggatttttt attatgcaag atatgaaaaa actaagagct
     1861 aatgtcactg aagacattca atggtttgat ttttacaaca aaactgaaat tacatctttt
     1921 cctaaagtaa tagagcattt tgaaaaacat attaaaggta aaaaattttc tattctttac
     1981 aaaagtataa ccccacataa tactgaatca gattcattat taattagtgg agaagccgtt
     2041 ttatcagatg aagcttctga acatcaaaaa tatagcaatt tctactttag tgcaaattgt
     2101 ataatttcag aaaataaatt actcttagca aaattacatt tggatttaat taaaactgct
     2161 ggctatataa atgaatttga atcatattca atacctaaaa tgacaaatac acatcttgat
     2221 gaaagtaata ttttatcaca attttattca atgttgcctg taggtataat tcgctacgat
     2281 ttatttggtg atatgattat tacttatatg aatgaagaaa tgtttgatat tataggttat
     2341 acaaaagaac agttttttgg tgagatgggc ggaaatttaa gagcaattgt tcatccagat
     2401 gatttagact ttgtttataa aaaaagtttg gatatgattg acacaaataa aattgaacca
     2461 tttttatata gattcataaa acgagatggt caaattgtca cagttatgta tacacaatgt
     2521 aacctttctg caattgatgg aaggtcgatt gcccagggta tgtatgtaga tgtaaataaa
     2581 ataaaaaatt taacaaaaat ctagtacaaa gtattttatt ttggagaaat taatatgttt
     2641 aaagaaattt ttttaagaac ttttccagga tttctaatta taagagattc taactacaga
     2701 attatattta taaatgataa tttaaaaaat ttaattaaaa gttatatgca agataatcca
     2761 cttggaatga ctaatataga aatagctaag aaactccctg ataatattgc taaatttttt
     2821 acggattctc acaatattca attagattgg gaaaaaaatt atccttatga taaaatttca
     2881 aattggattt tagagtttaa aaaagatact acaagctatt ggaatgtttt agaatataaa
     2941 gtagatgttg atgaaaaaac ctacataatt actatggcca atgatataac aaaactctat
     3001 gaagaaaata agagaaatct tcactactca attactgacc cattaactgg agcatataat
     3061 agaaaatatt taaatgatag atttgacata tttataggag attatattgt actaattgac
     3121 ttagataatt tcaaaatgat aaatgactat gaaggtcata atgtaggaga caagatttta
     3181 tgtgattttg tttcactttt aaataaagaa ttaataaatt ctacttctat tataagatta
     3241 ggaggagatg aatttattgt tatattctct agtgatgttg acaaaaatta tgtttattca
     3301 caactagaag ccttaagaga aaatttttta aaagtattct ctaagtataa gtatctttca
     3361 tttagttatg gtgttgatac agtaaaaaga aatttaaaat tgacaatagt tgaattggat
     3421 aaaaaaatgt ataaaaacaa gaaaaaattt gataaaaatg attattaata ttgttatttt
     3481 aaaataaatt aaaattatct ttcttttttt aaacttgtac attagtttat aaatatgtat
     3541 ctatacaaga tattgacatt aagtactgaa gcatatattg tttcagttct tttttgttaa
     3601 aattaactta tttaacaaag atatcatagt ttaacaaagc ttttaattta aatatattga
     3661 tgtataatat aattaaaata taatagcaaa aagaaaggag gaatatcatg agcatactcc
     3721 taaaaaaagc acccaaatta gcaaaacata ttataacaag tttttatata aatcgggata
     3781 ttgatgaagt attaaaatac ctttgtgaaa atgttacatg gatcggacca ggagaacagg
     3841 aatttctcac ttcttttaat gaaataaaaa attattttta tgcaggtcaa gatgaaattc
     3901 cttcttgtga tataaataat gatatatttg aaactgtaag tgaagatgag aatagatgta
     3961 tggtattagg aagatatact gttcgaacta aagaaaatac tcaaatgatt ttagaggtaa
     4021 atcaacgttg tacatttgag ataattgagg atagaggaaa attattagta aagcacatgc
     4081 acatatcaaa cccttatggt gaaatgcaac ttgatgaata ctttcccact aaaattggaa
     4141 ctcaaagtta tgattatttg caaagattac taaaggaaaa aacagaagtt attgaaatga
     4201 ttacaaataa tatcaatggt ggcttaaaag gtagcaatga tgataataca tactcatttt
     4261 tctatgtcaa tgaaggattg cccaaaatat taggatacac atatgatgag ttcatggaaa
     4321 tgagtggtgg ttcagcagtg ggtgctgtat atccaccaga cttacctaaa gcactagaag
     4381 attgtgaacg atgttttgca aaaggaccta catattcatc tgaatataga atccgaaaaa
     4441 aagatggaac attaatgtgg gtattagatt ctggaatgaa atcattaaat agtgatggca
     4501 tagtaaaaat taatagtatt attactgaca taacccaact aaaaaacata gaatctgaac
     4561 taaaattaga aagagagcga tatcgtattg ctttgcaaaa tattacagat atcatgtttg
     4621 agtatgatat tgaaaacgat acccttataa aatatcaacg tgtagaaatt gacaaaaaaa
     4681 ttgaattaga aaattttgaa acaaaaaatt actccaaact attagaatct ggtaaaatta
     4741 ttcaccttga tgatattgga aaactactag aagtacttca tggaaatctt cgtgaaacaa
     4801 ttgagataag acaagtaaat tcattaacta aaaatgaatg gagatggatt cgtgttcaat
     4861 gttcagttat atatgatagt gaccataacc ctatcaaaac aataggcgtt ttaaaagaca
     4921 taactgaaga taaatctaaa ttagaaagct taattaatca ggctcaacgt gacccactaa
     4981 cacaattata taatcaaaga gtatcacaaa atcttattga agagtatcta tgtagttctg
     5041 attccaaaaa taatgatgct ctattagtta ttgatattga tgattttaaa actgtaaatg
     5101 atacttttgg gcatttagag ggaaatgaag ttttagttgc tgtatcaaaa atactattac
     5161 ataacacaca tgataaagac atagttgctc gaattggtgg agatgaattt acaatattta
     5221 taaaatcatt aacaaaggat ttaataataa aaattactaa tgatatttta aatgatgcaa
     5281 gtaaaataaa agtaaaggac aatcataaaa taacattaag tattggtatt gccttcactg
     5341 atgattcaac taaattatat aaagatttat tttctaaagc tgataaagct ctttatctat
     5401 caaaagcaga tggcaaaaat tgttatagta catacgaata ataaagctat ttatacgacc
     5461 aagaatgaac ctttaaattt aatttcatag tctaacaatc aaagagttga agctaatctt
     5521 atagctagtc aactagttcc tagaaaaaat ttaaacaata tatttattga tactgctcat
     5581 ttttctaaag agcaaattta tataaaaaat taaactttta tgtacataca gatactaatg
     5641 ataaacagtt atcattagta tcttctatct taaaacaaaa tgataattta tctatataaa
     5701 aaagtgcttt gttgacagag ttcacttttt tatataggta cagaatttaa gtataaatac
     5761 atgttttatt ctatacattt tagttaatgt aaaatttctt gattgtactc tctaactttt
     5821 caacattata ctatttatat accacttagt taatataaac cccttagctg gttctagtgc
     5881 tttttctatg attttagtca tatttacata ccacttagtt aatataaaac caatttagat
     5941 gatgtatatg tgcaaaagga agaaataatt tacataccac ttagttaata taaaacggtc
     6001 actacaactt atacttccag tctaacgatt tgaatttaca taccacttag ttaatataaa
     6061 acatattttg gatttattaa tataaacata attaaaaaat ttacatacca cttagttaat
     6121 ataaaacccc aaaataaact gggcattttc aatacttaca catatgcaac tctcttaaat
     6181 ttgcagtgaa ccatgagtag tgcaaatgat aaagcttatc atataccctc aacaccttat
     6241 atttcaagcg ttaagctcta ctttgtaaca aatatcgctc actgcaaaac tatttacatt
     6301 tttattatat cataaatata ttatttttga atatctatac caatttgtgg tataataaaa
     6361 gcaagaagaa ctacaatcta tttagacgta gagtgaagtt caaatactaa tgtttattct
     6421 ttttgaactt gatttttagt tcaaaactaa agtcactcct gcctgtgtga actatacccg
     6481 ctgttttata cagttaaaac atgtaaaaat agttataaag tactagtgta actcattagt
     6541 actcttttta ttatattcat ataattaata tctatatttt aaagtttact atctaaaaaa
     6601 gagcacgtca aataaaaccc tagatattta atttttttag agatacttaa aataaattat
     6661 gctctctctg tacttaaata tttcctaaat tcaaatggag acatagaatt taatctattt
     6721 tgatatctaa aattattata gtagtatata taatcatcta ttgctttaac taattattca
     6781 tatgtatcaa atttccttaa atagtacatt tctaccttta aaattcccca aaatccttcc
     6841 ataggagcat tgtcaaggca acgaccaact cttgtataat atatgtatat ctaacaccat
     6901 taagaaatat atattttata atatatattt cttcatttaa tgcacttaga aaagcaaaga
     6961 aaatacagat tagtacactt tttagagaaa aataaaatta atacatctaa aatattaaaa
     7021 aatactttag ataatctaaa gacacatctc aaagttttta actaaattat aaaactaatt
     7081 acaaatgcta tgtaacgata ataaataaaa aatttacttc gcttaagcga cgtgtcgttg
     7141 aaatatttca tgttgccaac tggttcgtaa gttgataaga aaagaacact aattatttag
     7201 agttcttttc ttagctacaa gtggattata atacttttaa atctgtgaga aaatcatata
     7261 taataatgat atgaacaagt aaagaaaata caaaaatgac aaaattataa ataaaatatt
     7321 ataaaatcat aaggtcataa ttgttatatt gtagaaacaa agtaaagaga gagggtggag
     7381 aataaatgaa tcaaaaaaaa ataaagatta gtattaaaat ggtaattata ggtatatttg
     7441 ctgtaattat tgcttttgta ttatccagat tattcgttga gtatattgat gaattattaa
     7501 ggtctaattg gcaatatcag ctatttgaaa gtgagagttc atatatagta tttgccaatt
     7561 gtattatgtt ctcgaatata ttattagaaa tatttttaat atatatttgt agaaaattta
     7621 gaaaaatcta atatggtcaa cgtatttcga atcgaatact tcatgattat ttagtctatt
     7681 gtgctagtta aagtttaatt cccatataaa gtaaattgat tttagtaaaa caaatcaccc
     7741 ctaagggtta gtttatggat aagtcatcca aatattaact ctttaatttt tgctatagat
     7801 tcatctttgc tcataacttt acttataaaa aatgaaatat tataagctag aacttttatt
     7861 ctaattctag tcataaatcc tagcatagag ttactttttt tatattataa ataaaactat
     7921 acaacaaaga taatatttag taaaaataac tttcatttta actaaactta aaattcaatt
     7981 actctctaaa ttgacgttta ggaattagtt caaaataaaa aagagtgtat atattgaaat
     8041 tactacattt caatatatac actcttttta aatttctcag ttttccatgg aactttactt
     8101 tatttatttc tgtattagtt gtatttttta tgcatttttc tttaaaatca tattttctga
     8161 ttcaactctt atattataaa atttctttac taaattattc tctttttcta tatcttcata
     8221 tatatttttt aatcgccttt tacctaatct tttatctaac attactaatg atttaactaa
     8281 tatattatat gattttatcg catcttctat tgagatattt aaacatatat ttactgcttc
     8341 ataaaaatca aattcattaa aaaatccatt tttatttaca atcttatatc cctttgcatc
     8401 tctttctaaa tactcttcct aaatactctt caaaactttc atcatctaat ctaaatccta
     8461 tatcactatg aaaaataggt tttgcatttg gatatagttc tattgtttta tcaaaagttt
     8521 cgaataccaa gttattatta ttcgattttc caacagtcca ctataaacaa taataagttt
     8581 gttgctccaa ataatgtaac acttgtagaa agatgttaca gatatataaa agatagagaa
     8641 tgtagttttt ctcctaatac tattagaaat agaaaaaata tgattaaaaa tcaaattgaa
     8701 gttttcttta aagatatgaa attaatagat ataactcctt ctattttaca gtcttatata
     8761 aataacatat acaatgagca catgttaaat tcaacaaaaa accaagtcga ttttataaaa
     8821 tcagtattga aagaatctta tagattaaaa gaaatatcag aaaatatttg tgattttgtt
     8881 acaaccccaa tcaaaaaaaa tagttcaact tctaagcttt atacaaaaca aaaagctcaa
     8941 ttattattag aaaaatcaaa aaatattcct ataggtatac caattttttt gatgctaact
     9001 cttggattac gttttggtga agctgtatca cttatatggt cagatgtaga ccttgataaa
     9061 aaatctcaca tgtaaatcaa acattagttt atgtatacaa taaaataatc ttcaaaggcc
     9121 ctaaaacccc tagattcaaa agaagattat ttgttcctga tgaacttata agtttgttta
     9181 aaggggaaaa attaaaacaa aataagttaa aattacaggg tatattgaaa aatgaatttg
     9241 atttgatatg cttaaataaa agattcaacc cgtgagttca accaactttt gttgttcatt
     9301 ttaaaaaact attaagagat aataatccta gagatataaa agtacatgaa ttaatacatg
     9361 caaatgcgac tttgatatta ctttcaggta ctaacataaa agctatttat aaaatattag
     9421 tacatgcaga tataaaaata gctatgaata tatattccat attctcactt attagatgaa
     9481 atgaataaag aaacatctga aaatataagc aaaatactat tcaaataatg ttcagtttta
     9541 tgtcaattaa atgtcactca aagtgctttt tttccatttg tcagttaaat gtcacttaaa
     9601 gtaccttttt cctatttgtc agttaaatgt cagtaatata taaataagat atatttataa
     9661 gacccttaaa atcactatta tttaataaaa tttataatta tatactattt gctgttgaca
     9721 ttggtattat accatagttt atcatttata ttagaataaa attattaatt aatagtttcg
     9781 taattttata atagatttac agggagtgat aataaatgga tacaaaaata tctataaacg
     9841 aaaacatgag tctaggtaaa agatttttta aatacctagc accatctgta gttgcaatgt
     9901 gggttttctc actatataca atggttgatg gaatatttgt aagtaaagga gttggagaat
     9961 tagctttagc tgctgtcaat atatcaatgc cttttattaa ttttatattt gcagtttctc
    10021 tattattttc aactggtgca tctaccataa ttgcaatcta tctggggaaa aaagatatta
    10081 aatctgctaa cgaagtcttt tcatttaact tagtatcaat aattatatta tcaataatta
    10141 tattagctat aacttttttt aatttagata gattagcact atttttagga gctacagaat
    10201 ctacaattgg tatggttaaa gattatttag gaataataat attttttaac ggattcttta
    10261 tagtgtctta ttctttagaa gttataataa aaactgatgg ctttccaata ttagctactg
    10321 ttggtgtaat aatatctgct cttacaaaca tcatacttga ttacttgttt gtaattgaat
    10381 ttggatgggg tgtaaaaggt gctggtattg caactggttt gtctcaagtc ttttctacta
    10441 ttttcttctt aatacatttt ttaagaaaaa actctacttt gaatttttca aaatttagaa
    10501 ttgattttaa gacacttaga aaaattgtat ttataggttt tcctgattca actactgaat
    10561 taagttgtgg tatagttgtt ttacttttta acttaagtct tactaaatat attggagaaa
    10621 atgctcttat ttattatagt gtcataaatt atataaatac attagtactt atgactatga
    10681 tgggtataac tcaaggtatg caaccactta ctagctttta ttatggtgca ggaaatatag
    10741 ataatgtaaa aaaattatta aaaatgggga taaaagccac aataatagca tcagttgctg
    10801 tatttgcaat ttgtatggct ttttcaggac caatagtctc tttatttata catccagaag
    10861 aaacaatgct attcaatgaa ggagttcgtg tatttaaaat attctctata tctttcttat
    10921 tggtaggaat taatgtaata atttctggat tctttgtatc agtagaaaaa ccatcaattt
    10981 ctactgttat atctcttggt agagggcttg taatagttgt tttaagtcta atttcaatga
    11041 tactaatatt tggtggacaa ggaatatgga tgactactat tgtttctgag tttatatgtc
    11101 ttattttatc attagttttt ctaaaaaaga atttttctac attagactct aatctaaata
    11161 aggttgctta gatgataaga gtgacaatat atgaaaatat ctaaataaaa aatacctatt
    11221 atacaatctt gcaaatatta aacctttgca aaaattagga ttatataata ggtattttat
    11281 ttatgtaatt attttatttc taaatatcca tagccatctt ttattaaagt tccttttact
    11341 tcatattcta caggtttttt aaatattgga ccatctacga caaatgtatg atactctcca
    11401 tgttctccac atgggtctgc accagcattt tttatcttct ccacaagttc tctagttaat
    11461 ttttttccta aaaattcctc tcctaagtgc tttaagttta cttttgttac aacactacaa
    11521 aatccactgt ctataaattc atatactaaa tcctctctac tctcttgcca taatggtaaa
    11581 tctactttca taccaactgc ttcacatcta tcagtatccc attttctatg actttctata
    11641 tctatatctc catatgcaca tatcgttgct cccatgtttt tagcttttat taaagccctc
    11701 tcaaagctct cttcatatcc ttctacgcca cactcagcaa ctaataaagg taatcctatg
    11761 ttgctactta cttgcttaag catattatat tctaaattat gtgtccatga ctttccatgt
    11821 tcttcactta tagttgtcaa gagtgccaca ggcttatatc cttttttcaa cattcgattt
    11881 aatgctaata tactatcctt accaccacta aatgacatta caaatttttc atcagccaat
    11941 tttaacacct ctttcatcta actttgtttt attaacataa ttataccaaa tttttgagtt
    12001 gcatagcaaa ttatattaaa ttgttaattc actataaaga atgtccctaa tattaaaagt
    12061 ataaccccta tgtatttgta taaatgcatt gtttcttgtc ccaaaattac gctatcaata
    12121 aaaaatccaa ctaaaacttg ggatacaaga gttaatataa gtgtatttga tactcctaaa
    12181 tttggaattg cctttactgt aaaatatatt attagacctc caaatactcc tcctaataac
    12241 aacttataat taaaaccatt tatagatact agacacttta agtcacctgt aactattata
    12301 cttgccaaaa aaaatattgc tcctacagtt aagcttataa aggtggccac taatgctgtt
    12361 gtactttttc ctagctctcc atttataaaa gcttcaagag ctgtagaacc acctgctaat
    12421 aatgcaaata ttattgccca taattttgtc atggacaaac ctccttctat ataattctat
    12481 taatagaagt ttatgataca acctattaat taattactaa gtatagttta atttttatac
    12541 actaggcttt atttggatat gaatttctgt tataattgtt ctatcgattt tggaggtgat
    12601 tttatgtaca caaattttaa tatcgatttt aataacttta ataaaaaaga aaatgctatt
    12661 aaatttatgt taatgggagt attactcatt atattaggat tattatgcct tacatttaag
    12721 acattaggta taaaattaat ttcttggact tttggtattg cacttttatt ctttgcatat
    12781 ttaaatttaa aaaatattaa tgagttaaaa agatatgcta ctaaagaaga aattaaaccc
    12841 tctattaata tccaatgggt tctaataatt gcatgtatat tactttttgt atttcctcaa
    12901 aaaatacaaa gcatattttc attattttta ggattttatt taatctttaa tcaactagta
    12961 gcacttgtaa atagtaagaa taatccttat tctaaattta caacttggaa tatagttaag
    13021 attctatttg gtatatgcct tatattatct ccactttttt tatctagatt tatagtatcg
    13081 ataatgtctt ttttcataat attatttgga ctagttctat ttttctctgg aaatacagcg
    13141 agaaaatatt aattattttc tcgctgtatt tttatattac ttaaaataga atttgtttaa
    13201 gttatctatt tgtggtaata tgttttttgt acaagctcag cgtacaattt tattagaaat
    13261 aggtgatttt ttggataaat ttaaatatgc ttttgataat aaaagatatc atacttggaa
    13321 ttactatctt agaaatactt ttggagaaaa agtttttaag gtatctataa atgcaggatt
    13381 tacttgccca aacattgatg gaagtcttgg ttatggcgga tgtacctatt gtagcaaaga
    13441 aggctcaggt gattttgctg gaaaccctaa agataatctt atcagtcaat tttataatat
    13501 aaaagaaatg atgttaaaaa aatggcctca tgcaaagtat attggctact ttcaagccta
    13561 tacaaatacg tatgctcctc ttgaagtttt aaaagaaaaa tatgagacta tattagaact
    13621 tgaagatgtc ataggtcttt caatatccac aagacctgac tgtttgccag atgatgttgt
    13681 agaatactta tctgaactaa ataaaagaac caatttatgg gttgaacttg gacttcaaac
    13741 aattcatgat tctacatcta aaattataaa tagaggtcat gattataaaa catttgttga
    13801 aggtgttgaa aaactaaagt ctaaaaatat aaaggttgtc gtacacatta taaatggtct
    13861 tcctggtgaa gattataata tgatgatgga aacagcaaag gcagttggta aaatgggtgt
    13921 agacggtata aaaatccatc tattgcatgt tataaaagat actcctatgg aaaagatgtt
    13981 acaaaatggt atgttaactc ttatggaaca agatgaatat atcaatcttg tctgtgacca
    14041 attagaaata cttcctgaaa ctatgataat ccatagactc actggtgatg gtaagaggga
    14101 tgaactagta ggtcctatat ggagcctaaa aaaatgggaa attttaaatc aaattgatga
    14161 tactttaaag gctagaaatt cttatcaggg ctgtaagttt gtttaataca aaataataac
    14221 tatagaaatg gttatataaa tatagtcctt tctatagtta tttagtattt acaaaaacaa
    14281 aatattaatt tgtacattta tacaatatat aataaaaaat agatagtcta aactgtatct
    14341 acttatattt tatttgtact gtccacttta ttctataaaa tttataaata gttatttttt
    14401 aataccaaat ataatttggt ataatttttg ttggactcta aaatgtatac caataaatcc
    14461 tttttaagta tataattcta aatactttca gttttttcaa tctgctttaa cttgttggac
    14521 ttataaaata ttcttatttc atcataagaa agattaagtg ctactactgc agatattaac
    14581 atcaacatca ctccaaataa cttccctaag gaaaaacttt ctcctaatat agtgcaacca
    14641 attattactg aaccaactaa ttctactgat gccacaacac ctgcaactga caactctaca
    14701 cctttagata tacctgctgc ataaaaaata tatgcaagtg cagctggaac aatgcctaac
    14761 attagcatac aagataaaac atctaaatcc ttagcataat ttaagatttc ccaaggtctt
    14821 gaagaaggaa tcataaatat agctccaaat aagaaactgt atactaatat tgttgaactt
    14881 gaaaattcct ttaaagcatt tttactaata gttggcataa gtgcatatgc tattgctgat
    14941 aataccccaa gtaaaagtcc aaaagcgttt aaaccttgaa aatccaaacg tccaccagtc
    15001 actgccataa ttgcaccaat aaaacaaaga ataagtgaaa ataatttgct tctagtaata
    15061 ttttctttat aacatatttt tgaaaatatt gctaaaaaca gtggtgatgt atatagtaaa
    15121 actgctgcaa ttgatacacc cgcaatgtca attgctttaa aataacataa attaaacatt
    15181 gcctgacata taatacctat tattactgaa tatataattc cttttttgct tatctttaag
    15241 atttgtggtg tccttataga tgaatatacg cctaaaacta taaatccaaa aaacaatctt
    15301 gtaaatgcta cttgttctga tgtcagtcca tttcccatta aaaactttcc aaataatcca
    15361 agtgtagccc ataataaccc tgctattgct ataaatacat accctttact tttttctccc
    15421 attttaaatt cccttctatt tttatttagt caatttacac tcttcttttt aagcaaaaac
    15481 tatgccatct taaatcaata tatatcgact ctaaaaccct agtctttcca ataatactaa
    15541 taaattttca atttttttat atttattttt cacttacttt aagttgattt agacttattt
    15601 taattgtaaa atgagttata tataacttat ttaaagttaa aatagactga aacttatata
    15661 taaatatcaa attttaagta tattaatcaa ttaaacctat tttaatatat cattaaaaat
    15721 ttaggaggaa aatataaaaa tgaatttaga tttggaattt tatcaaaaaa tattagaagc
    15781 ttcccatgat gaaatatgtg tttctgatga taaaggaata ataatatatt gtaataaagc
    15841 atttgaagaa aattatggtc taaaaaaaga agatatttta ggaaaaaatg tttctttttt
    15901 agaagactct ggatattcaa caaaaagccc cataccaatt gtattaaaaa caaagactaa
    15961 attttcactg gaacaagata ctcaaacagg gaaaaagctt atcataactg ctacccctat
    16021 atttgatgaa aatggaaact tagaatttac tgttgaaaat tgtagagata taactgaact
    16081 taataatata aaaaataaac ttgaagacac aaaaaagcaa gtaaaaaaat ataaatctga
    16141 agtagaaaca ctatacagaa ctgcacttag aattgaggat actgtaatta tggacggaat
    16201 agttatgaga ccaatcataa acacagtaaa tcatgtttct aaaacggatg taagtgtact
    16261 tttattgggt gaatctggaa caggaaaaag ctcacttgct agatatatac accacaacag
    16321 caatagatct aatggcccat ttataacaat aaattgtgca actatatctc cacaacttct
    16381 tgaatctgaa ctttttggat atacttcagg tgcttttaca ggtgcaagta ctaaaggtaa
    16441 agttgggctc gtagaattag ctaatggagg aactcttttt ttagatgaaa taggagatat
    16501 accacaaaat ctacaggcaa aatttttgca attaatacag gatagaactt ttactccagt
    16561 tgggagctta aaaaataaaa aggtcgatat aagaattata tctgctacaa atgttgattt
    16621 agtttcaaaa gtaaaagaaa aaaagtttag agaagattta tactatagat taaatgttat
    16681 agaaataaaa ttgcctcctc ttagagaaag aagggataat ctagtggaaa ttataaaata
    16741 ttattttaat aggtactcaa gtgacttcaa tctaaataaa actatttcta aagaagctat
    16801 ggatgctatt gcaaattata gattcccagg caatataaga gaattacaaa atataattca
    16861 aaaaatcttg cttacttgta cagataatca tataacaata gatgacttac caaacattct
    16921 tacaaaaaat atacatatta ccaataatgg aaataaaaca catatatccc aaattaataa
    16981 ggttattata tctgactcaa aatctactaa ttataaaaat aaaaattttg acactttaat
    17041 taaagagtat gaaaaaaata taattttgga tgcttatgaa aaatttggta gttcttataa
    17101 agtagccaaa caccttgaaa tctcacagtc taaagccaat aggttaatta gaaagtatac
    17161 taatacttaa ttttaaattt gataaataaa aacttaacac tatttacata ttgtaaattt
    17221 ggccatttac tgttattatg aatatggttg aaattattta aataaatact gttgacttag
    17281 tttcat
//
LOCUS       DAEOSP010000064        17263 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.064, whole genome shotgun sequence.
ACCESSION   DAEOSP010000064 DAEOSP010000000
VERSION     DAEOSP010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.064"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            57..1898
                     /locus_tag="KRH54_001775"
     CDS             57..1898
                     /locus_tag="KRH54_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBG8579439.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFNNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKTEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            2203..2910
                     /locus_tag="KRH54_001776"
     CDS             2203..2910
                     /locus_tag="KRH54_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8579440.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            2904..4214
                     /locus_tag="KRH54_001777"
     CDS             2904..4214
                     /locus_tag="KRH54_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8579441.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            4331..5014
                     /locus_tag="KRH54_001778"
     CDS             4331..5014
                     /locus_tag="KRH54_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8579442.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            5007..7394
                     /locus_tag="KRH54_001779"
     CDS             5007..7394
                     /locus_tag="KRH54_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG8579443.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYDDYKSDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKLNDKSNLSI
                     EQAKSKLNKIANDVGLDTINVAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQVKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            7674..8816
                     /locus_tag="KRH54_001780"
     CDS             7674..8816
                     /locus_tag="KRH54_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG8579444.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            8835..9359
                     /locus_tag="KRH54_001781"
     CDS             8835..9359
                     /locus_tag="KRH54_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG8579445.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIDRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            9459..10277
                     /locus_tag="KRH54_001782"
     CDS             9459..10277
                     /locus_tag="KRH54_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG8579446.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYIASNIHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSKLFAKKLEESLNPTKAIEKVVLCLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFSGENKTIKENIKYSYL"
     gene            10355..10666
                     /locus_tag="KRH54_001783"
     CDS             10355..10666
                     /locus_tag="KRH54_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBG8579447.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSVSSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(10843..11526)
                     /locus_tag="KRH54_001784"
     CDS             complement(10843..11526)
                     /locus_tag="KRH54_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579448.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(11619..12260)
                     /locus_tag="KRH54_001785"
     CDS             complement(11619..12260)
                     /locus_tag="KRH54_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8579449.1"
                     /translation="MGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDGLSR
                     GAIYHHFKSKDEIIKAITSRLYNDGFYGYEVKNDEGKTALEKLQYRLLHAYEQHNKQD
                     IFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMEVKNPEIAAQMFGFL
                     LNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            complement(12434..14437)
                     /locus_tag="KRH54_001786"
     CDS             complement(12434..14437)
                     /locus_tag="KRH54_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBG8579450.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELMKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(14590..15228)
                     /gene="recX"
                     /locus_tag="KRH54_001787"
     CDS             complement(14590..15228)
                     /gene="recX"
                     /locus_tag="KRH54_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBG8579451.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            15504..16193
                     /locus_tag="KRH54_001788"
     CDS             15504..16193
                     /locus_tag="KRH54_001788"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBG8579452.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            16194..16766
                     /gene="yqeK"
                     /locus_tag="KRH54_001789"
     CDS             16194..16766
                     /gene="yqeK"
                     /locus_tag="KRH54_001789"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBG8579453.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            16883..17233
                     /gene="rsfS"
                     /locus_tag="KRH54_001790"
     CDS             16883..17233
                     /gene="rsfS"
                     /locus_tag="KRH54_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBG8579454.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
BASE COUNT         6747 a         1961 c         2800 g         5755 t
ORIGIN      
        1 tgtaaaatat aaattttata ttaaagctaa aactaatttg aggaggctaa tttatattga
       61 agattagaag gtatttaatt ttggtaatta taattctaat tacaacaagt cagagtttta
      121 tttcttatgg gctagagcga tttgaagaaa taaccatttt tcacactaat gatatacatg
      181 gaagatatgc tgaaggtgat gaccatatac aaataggaaa tctagccaca ttgaaaaaag
      241 aaactccaaa ctcaatttta gtagatgcag gagattgttt gcatggactt ccaatagtaa
      301 atatggatag agggaaaagt gcaattgaat taattaaatg tgctggatat gactatatta
      361 ctcctggaaa tcatgacttt aattatggaa aagatagact gtttgagtta tctaaaagtg
      421 ctaatttagg agagaatact ttaaaatttt tatcatcaaa tatttttgaa aatggaaaaa
      481 aagtttttga tagtaatgat ataaaagaaa tagatggaat aaaaatagga ttttttggac
      541 tctcaaccca ggaaacaaag agcaaaactg gatttaaaaa tgtggaagga ttagagttta
      601 aaaatcctat agaaagtgca acagaacaag taaatgaatt aaaaggaaag ggtgccaatg
      661 taatagttgc aatatgtcat attggtacaa atagtttaag taagccaaat agtatagata
      721 tagcaaatga ggttcaagga attgatttga ttattgatgg acacagccac actaagtttg
      781 aaaatggtaa acaagtgggt gatacattga ttgtaagtgc aggacaatat ttagaaaata
      841 ttggcgagat aaaactaact ttggatacta atgatgtaga aaatataaaa atagaaaata
      901 aatcagttag attaattgga aaagaagaag cattaaagta taagcttgac tccgctgtaa
      961 gtaaaaaggt tgatgaaata aaagaacaac aagaaaaaat attagataaa gttatagggg
     1021 taactacaaa tactctagat ggttcatatg aaaatgtaag gacaaaagaa actaatttgg
     1081 gtaatttaat aagtgacatt ttactagata aaactaaggc tgatatttct ttatttaatg
     1141 gagggaatat tcgagatacg atagaaaaag gcaatataac aagaagagat attgtagatg
     1201 tatttccttt taacaatact atagttacca aagaacttac aggagcacag attaaagatg
     1261 tattggagca tggagtaaaa ctttacccag aaaaaagttc agcctttttg caagtgggag
     1321 gaatttcata ttactttaat cctaaacaaa aagaaggcga aaaaataaca aatattcaaa
     1381 aagaaggaaa gccattagat ttaaataaaa agtatgtagt tgcaacgaat gattatatag
     1441 catcaggtgg agatgaattt ccttgttttt caaaagagcc agtactaaag gaatttggaa
     1501 atttagagag tgcagtcatt gaatatatag agtacaaaac agaaatatca aaaaatgtag
     1561 atggaagaat tggtattaaa tctaaagatg agttaataag caagaatcat gagagtgatt
     1621 tgatattaag tgaaaatgaa aaaaatatca atgataatga agagaatcta tctaataata
     1681 taaaaagctc tgatagtgat aaaaataaaa gcttagataa aacaagtgct agagaaagtt
     1741 ctaatctagg aatatctaaa agagaagcta aagaaaaatc tcctaaaaca ggagatttag
     1801 gtttttcaaa tagtataatt atatttatag tttcaagtac attgatatgt ttattgaatt
     1861 ttaatcaaaa agagttaaag gataaaaagt caaaataaaa attaatgagc aaaaactagt
     1921 agatagtaat ttaattttag ataatagtaa acagcaaagg aaattagtta tataaatact
     1981 aatttccttt gttttatgtt ttaacatgat tacttttaaa taaaattgta ttttatgaaa
     2041 aataattaca ctaaatggca tagtattatg gggattttta gtttttattc aagtaaatga
     2101 aatcatatta acaacttctg atatatttac aaattgataa aaaaatgatt ttattagtat
     2161 gatataattt agctagtata tatgatgatg gaggatatag ggatgaataa tatattactt
     2221 ttggaagatg acaaatcttt aaatagagga attagtttta aattaaaaaa agaaggatac
     2281 aatgttttta gtgcatttgg tatagaagaa gcaaaaaata ttttcgcaaa agaagagata
     2341 tgcttaatta tttccgatat aggtctacct gatggtagtg gttttgactt ttgtgaggag
     2401 attagaaaga aaagtgatgt ttacataata atgttgacag ctcttgatga agaggtggat
     2461 attgtcacag gatatgattt aggtgcagat gattatatca caaagccttt tagcttgatg
     2521 gtattaatat caaaagtgaa tgcgcttatg aaaagagtaa atacagtaaa aaattatact
     2581 ctattagttt gtgatgattt attcttttat tatatagaaa ataaattaat tataagaaca
     2641 gatgataagg aagaagagat aattttaagt aagacagaga caaaactatt aaaatatttg
     2701 atggaaaatt ctatgcagac attgactaaa gagcaactgt tagaatcttt atgggatagt
     2761 agtggtaatt ttgtagatga taatacgatt gcagtaaata taagaagact tagacaaaag
     2821 gttgaaaaaa atccatctga acctaaatat ataaaaacag taagaggggt tggatatatg
     2881 tggggagaaa ggagtataaa aaaatgctaa aaaatatgaa cctcaaagat ggtttaagtg
     2941 tgaaagatga ttatataatg agaagagtta ttgtatattc tgttttatct gtactaatag
     3001 taacccttgt atttatactt tttgaatata gcagattaca taaattgtat gaacatcaaa
     3061 taaaaaatac tcaaaatatt gctggaatat tgattgaaaa atatcctaat gatgaaatag
     3121 atatagtaaa ttctatttat aattctgatt atagtaaggt gtctactgga gaaaaggcat
     3181 ttaagaaatt tggctatgga ttggaaaata aaatgtcaaa tgataaaaat tttagcttat
     3241 acttaaaatc tttttttaag gaatcatttt ttattttttt aatgatgttt atagtagtta
     3301 taggtataat atattatttt ataagataca taaataaaag attgagtaaa atttatttta
     3361 tagtagataa gatgtcacaa gaaaactact tgaaggacac tactgagtca gaagaaactg
     3421 gttatctagt agaatataat gaatacttta aagaaggcat atttagtaag ataaataact
     3481 gtctatatga gcttaataga agtttaaaga taaagtttat taaattagaa aaagaaaaag
     3541 aaagtgtaaa atcattagta acagatattt cccatcaatt aaaaactcct ttagcatcct
     3601 taaaattata caatacatta cttatagaag aagaattaga agaagatgaa gccaacgagt
     3661 tcttattgac caataaggct tctataaata aattagaaaa tttaataaat tctttagtga
     3721 acatatcaag gttagaaatt agcatgataa acataaaaaa agaagataat gatataaaaa
     3781 atactattct aaatgcagta aaaagtgtta cccctaaggc taagagcaaa agtataacat
     3841 taaatttaaa tgaatttaac agtagaatta taccacatga taaaaagtgg actgaggaga
     3901 gtatttttaa tgtattagac aatgctgtaa agtatacaca ggtaaatgga gaaataaata
     3961 tatctgtaga agaaactgct aatttcttta aaattataat agaggataat ggcataggga
     4021 ttgaaaaaaa tgagtttaat aatatattta aacgttttta tagaggttct gctgatgaaa
     4081 ttgaatctgt agaaggtagt ggggtaggat tgtatttaag tagaaagatt ttagaagaac
     4141 aaggtggaaa tattatagtt tcttcaaaag taggaatagg gagcaagttt tctttgtttc
     4201 ttacaactat gtaatattaa atgaaaggaa aatgtaagcc tctacattta ttatataagt
     4261 ataaacagct ccagaaataa aaaattaata aaatatgggg taatataact tagaatgaga
     4321 ggataatttt atgaaaatac tatatactga aaatttatca aagcattatg gaaaaggaga
     4381 aagcttagta agagctttgg acaatgttga tttagaaata aatgaaggtg aatttgttgc
     4441 aataatagga aaatcaggtt ctggaaaaag tacacttttg catatgattg gaggtcttga
     4501 tatacctaca tctgggaagg tgtatataga taataaaaat atatttacat taaaagaaga
     4561 agaacttgct gtatttagaa gaagaaaaat agggtttata tttcaaagct ataatttaat
     4621 tccatctctt aatgtatggg aaaatgtagt gttgccaatc ggtttagatg gtagagaggt
     4681 agatgaatct tttataaaag aacttttgaa atcattagga cttgaaaata aacacgacgt
     4741 tttaccaaat acattatcag gtggtcaaca acaaagggtg gcaatagcaa gagcattagc
     4801 tacaagacca gctataatac ttgctgatga accaactgga aacctagatt ctaaaacatc
     4861 tgatgaggtt atgagtatat taaaatctat gtctaagaag tacagtcaaa ctctagttat
     4921 gataactcat gatgacagta tagcccaaat ggctgataga gttatattca tagaagatgg
     4981 cagggtgtca aaggttggtg ataaaaatga ttaatactac taaaatagca aaaagtaatt
     5041 taaagcaaaa taaatcaaaa agtattctaa taataattac tataatactt tcaaccttaa
     5101 tgctttcttc tattggaatt tatattgttg atgcaggagc atatcaaaaa gaaaatacta
     5161 taaagtattc aggcaattat caaggtatat tagcaaatgt agatgaaaaa caggctgata
     5221 tattgagtaa tcatgcagat gttgaattaa ctggtgaaat gaatggagta ggtgttgaaa
     5281 aactggaaga tgattcaaat atttctctag catacatgaa tgaagatgct ttgaaattaa
     5341 atagttttga atttatcaaa ggaaaattgc catcaaaaga gaatgaaatt gtgttagata
     5401 gtggagcttt aaaggcactt ggttataaaa ataaagattt aggtgaaaag ataaaaatat
     5461 cttatgatga ctataaaagt gataaaaaaa ttgaaaaaga gttcattata agtggaatct
     5521 taaaaactag cgaaatatct gaagcaggta aatattatta tgctattata tcagaatcat
     5581 atatgagaaa tactagagat atgtcgcaag aagattttaa tatatatgtg aaattaaatg
     5641 ataaaagtaa tttatcgata gaacaagcaa aatcaaaatt aaataagatt gctaatgatg
     5701 taggattgga cactattaat gtagctgtaa atgaaaacta tataaatgct ttaaaaccag
     5761 atatggaaac aattatgggt ggagtatttg tagggttagt aattgtattg tctagtattt
     5821 tagtaatata caatatattt tatatttcaa tagttacaaa ggttcaagaa tttggaaagc
     5881 ttagagcaat tggtgcaact aaaaaacaag taaaaaatat agtatttaag gaagggttca
     5941 tattagccgg aatagcaata ccaataggaa taattttagg ctatgtactt gcaaatatta
     6001 taataaaatc atttatggat atagatgtaa aatcatcacg attaccagtt atattatcgg
     6061 ttgtggtaat aagctttata tctgttgttt tatcactttt aaaaccaatg aaagtagcat
     6121 caaaagtatc cattgttgac gcagtaagat atacaggaaa taaaataagt aataagagta
     6181 agagaaaagg atataaaaat ataaatctaa agagattatc acatgccaat ttagaaagaa
     6241 ataaaaaacg tacttacatg acattggcat cacttatact tagtggaact atatttataa
     6301 cagtatcaac tgccttagaa agttttgatg ctgagaaaat ggcaagagaa cactttcctt
     6361 atgatataga agttagacta tctggttatg aaatgaacag tgataaaaat cctaaagata
     6421 atttgaatat tttacaaatg gataatcctc taagtaaaga tttttttaat caaataaaga
     6481 acatggaagg aattaaaaga atagaatctg caagaagtgt aaaaattggt atggaggact
     6541 atgatgttga atttaagtat gaccttttac aaagtataaa tgaaaatgat gtaaaatcat
     6601 taaacaagaa tataatagat ggaaaaataa atcttgaaag acttcaaact ggagatgaaa
     6661 tagtaattac tcatgttgat acagcaaaag aaatgggagt aaaagcagga gataaaataa
     6721 gattaacttt atatgatgga gataaaaaga taaaaaaaga atttaaagtt caagcaatag
     6781 ctatgggggt tccaagtttt ggtataggga aggattttat tgatagaact ttgaaatacg
     6841 attcaactag tagcttggga atatatacag aagaaggaaa gtatcaagaa atcaaagata
     6901 gtatcaagaa gattgctaaa aataatggat ttttagagac tgattttata gatagtagaa
     6961 ttgagtcaaa taaggctact atttcattta taaagataat gggatacacg ctaacaggca
     7021 taataggtgt gattggattt atgaatcttg tcaacacaat gataactagc atagttacta
     7081 gaaaaaaaga acttggtatg cttcaagcta ttggtcttac taataaacaa ttagtgaaaa
     7141 tgcttaattc agaggcaatg tattacactt ctggaatgat gataggaagt attctatttg
     7201 gaggtatatt agggtatatt gctgttatat ttctaaaaaa gactggatta tcttatgcaa
     7261 catattcttt gccaatagta ccaatcttgt tgatgattgt gtgtattctt atagctcaat
     7321 ttataacaac ttatttgata ggacgaagtt ttaataaaga atctcttatt gatagagtaa
     7381 gatacagtga ataaaataca aatatttatg gttagtttaa ctagcaaaaa acatataaaa
     7441 aaatatagtt aaagagattc ataattaaat aggttaaagc atagtacaga gcaaacaatt
     7501 taagcagatg tttgctctgt attttattat gtaatcaata atatatctaa tatactgata
     7561 ttattcatgt tagaaaatgt accggtacaa ttctaaaaaa gtataaaatt gtaccatata
     7621 aaatgaaaat tttgtataat aatcgtatag aaaataaata ggagctgata tatatgaata
     7681 agaagattaa ttatgcacca ggaccaactg aaactaggga aaatgtaaga ttagcaagag
     7741 cagaaaaaac aacaaatcca gatatagata ttgattttgt tgaattttat aaaaagacat
     7801 gcgagaagtt tggtaatata attggaacaa aaaacgatgt ttacatattg agtggtgaag
     7861 gaatattagg tcttgaagca gcatgtgcat cacttacaga aaaaggagat agagtacttg
     7921 taattgacaa tggtatatat ggagaaggtt ttaaagattt tgtaacaatg tatggtgggg
     7981 agtatgtgct tttttcaagt gaatatacta aaagtataga tattgatgaa ttgaaagcat
     8041 ttttagatga ggatagtaat tttaaatatg caacagtagt gcattgtgat actccaactg
     8101 gcgtattaaa tgatgtaagc aaaatatgtc cattacttaa agaatatgga atattaactg
     8161 tagttgattc tgtagcaggt atggtaggag aaagattaag tgtagatgaa tctaaaatag
     8221 atataatatt aggtggttca caaaaagcta tttctgcacc agcaggactt actatagtcg
     8281 gaataagtca agatgcaaaa aattgtataa aaaatagaaa aactgatgta ataggttttt
     8341 attgtaattt aaatatatgg gaaggctatt atgaaaaaaa atacttccct tatacaatgc
     8401 caataagtga cataatgggg cttgataaag cacttgataa tatattagaa gaaggaatag
     8461 aaaatgtatt aaatagacat gaaaagatag cttatagtgt tagaaaatct attgaggaat
     8521 atggtctaga actattttta gaagaaggat attctaatac agtcacagct ataaaaattc
     8581 ctgaaagtat aggagcttta gaacttactg attatatgct taaaaattat aatacacttg
     8641 ttgcaacatc actaaatcaa tatatggata ctattttaag aataggtcat atgggagaga
     8701 atgcaaatct aaataaaata gaacatgtac taaatgtttt ggataaaagt ttaagtgcat
     8761 taggattcaa aggaaatgga aatttgttaa atttatttaa taagtattat ttttaataga
     8821 tagggggata tagtatggaa gttacagcta agcaaaaatt tacaacgaaa gacatggtgg
     8881 aaacctcact attaatagca ttagtattta ttgcaactaa atttataaac ataagattac
     8941 caatatctat aaatggtggg cttgtgcatt taggaactgc aatgttgttc atatcagcaa
     9001 tagtatttgg aagtaaaaaa ggtgctttat caggagctat tggtatgtca ctttttgatt
     9061 tgatatctgg gtggacttta tgggcaccgt ttacctttat agatagagga gttatgggtt
     9121 atttacttgg aaagattgca tgggcaaatg gtaataatgg gaataatttt ttaatcaatg
     9181 taataggaat atgtgtttca tcaatttgga tgctatttgg atattatgta acagaagtta
     9241 tactttatgg taattttata gtacctctta cttcaatacc aggtaattta atgcaagtat
     9301 tgataggtct tataatagcg cttcctattt ctaaggtatt gaaaaaatgt ataaaataaa
     9361 tataaagtga actaaaattt atgatagcat aaattatact gtataatatt aggatggagt
     9421 acataattaa aaaattttag tatagaggtt agttcacaat gttatcagtt acaagatatt
     9481 caatcaaaaa tgttcgttta aagccgtggg taaagtttat ttggtatttt gagacaaaat
     9541 caaatatact gttaaataat aagttacttc cgacagacag tatagatatt attctcaatc
     9601 tttctgatgt tatggaatat aaaattgaaa atcaagacta tattgctagt aatattcatt
     9661 ttaatggaat aagggataaa catggattta ttattcaaca tggaaatata cgtgttatag
     9721 ggatttcttt ttatccattt ggtctctatc catttttaaa gataccaatt tcagagttta
     9781 acagacaaat tgttgaccta gaagcagtat caaaactatt tgcaaaaaaa ttagaagagt
     9841 cattaaatcc aacaaaagca atagaaaaag ttgttttatg tttagaagaa gtattattat
     9901 ccatattaga agaagattta atttcaaata aatatgtaaa gcttctaaat tcatttatat
     9961 atgttaatag atatagcaat ataaaagcat tttgtgatga tacaaatata aatattaaaa
    10021 cattggaaag aatctgttta aaatatacag ggtatacacc taagatttta aagagaattt
    10081 acagatttaa aatggcaagc aatcaactta tttataatta taaagatgat gaactttttg
    10141 attttattta tgaaaatgag tactatgacc aagcacactt tataaaagaa tttaaaaaat
    10201 tttcagggac gtcaccaatt aaatttagtg gtgagaataa gacgattaaa gaaaatataa
    10261 agtacagcta tttataatta tactgtcgat tttttacaat acctccaatt tattttttga
    10321 tatgatatag gaaaataaat tggaggtttt acatatgaaa attattacag aaatagttga
    10381 gtttaatgtg gaagaaaatt tatcaaaaga agagtttata gaaattgtaa atgacttaga
    10441 attaaaattt cattctattc aatctggttt tattgatact gaacttttat atgatgaaaa
    10501 gtctcaaaag tggattatga tacaacattg ggaatctata gaagacctca agagtgtttc
    10561 tagtaaaatg tttaaagatt cttcaactga gaaatttaga aaagctttaa agaatcaaac
    10621 tgttaagatg aatataatac cacaaattaa aagttggaaa ttatagaact gaatgtaaaa
    10681 gatagttaaa tagtatcaaa ctaaattagc caaataacat tttgtattta aattgttatt
    10741 tggcttttag tgtttatttt aatatgagtg ttatatacta gaaaaaccaa caaactgtta
    10801 gagtgtggat tatcataatg tcatattcgg gagctaaaag gcttaatact gatttgccaa
    10861 ttggtcaatc catgcatgtc catctggata tgctgttatt aaagcattta caagccaatc
    10921 cctttgctct tgtgataata gtgggactgt tacatcgaag tctttttgat aaccaagcct
    10981 atctatgtat attgatttgc aaaacaaaac cacttcggga gccatatatg ggatattatc
    11041 tgatgttatc agtctggcct tatccatatc tcttataatt ctttgatttt gggaaaaaag
    11101 aaattttcca tctttctgtg gattatatat caattcaata aaatcgaact gtaactgttc
    11161 gtcacactgt atattcattt tatagttatc atcctcgact ttttttaatt caataaatga
    11221 gcaatcaggc ttaatcgcaa aaacacaaat ttgctctata tgttgcttgt taaggtcgat
    11281 aataggtcgt aataaataat tacccaaggg ctcatatata tcccatcctt gattaagcat
    11341 atactgaatg ctcttttctt tatctgtgtc agaaatccat atgtcaatat cactgtgtgg
    11401 gcgaatatgc atattcgcaa ataattctag ggcatatcca ccacatattg aatatgaaaa
    11461 gccacaatca ttaaaaaagt ttacacaatc tttaattagt ttgttcatag caacctcctt
    11521 ttgcatgtac tgaaataaat taaatttttt aaaataccaa ctaaatgttt gttttgtgta
    11581 aatataaaac tgccgatatt aaggcagttt tatatttact atttagaaaa tttattaaca
    11641 aatttttgat attcacacat tatcttatca ttcataaaag aaatacccat aaaagcagct
    11701 tgctcaccaa acaaagtaaa acgttctatc atctgttgtg gattacctgg ataaactgtt
    11761 ggaatacacc atgtgtttag aagaaaacca aacatttgag cagcaatctc tggattttta
    11821 acttccatag aattatcttg atttccttct tcaatcaatg cttttatcca aggcacaata
    11881 tcatttataa tctcatcaaa ctgcgtagct aaatacttag gattacgcaa tagtgatgat
    11941 gacattaaaa aaatgtcttg tttattgtgc tgttcataag catgaagcaa cctatattgt
    12001 aatttttcta atgcggtctt tccttcatca ttttttacct catacccata aaatccatca
    12061 ttatacaacc gacttgtgat tgccttaatt atttcgtctt ttgatttaaa gtgatgataa
    12121 atagcacctc ttgaaaggcc atctaaatta tctacaatgt cttgaatcgt tgtctgctca
    12181 tacccttttt ctaagaataa cttatgtgaa atatcaagta ttttttgtat agtattatca
    12241 ggttcttttt ttcttcccaa aaaactcact cctcgaaaca atcatttggt atgtttatct
    12301 tagcaaatac ataatgattt gtcaatgtgt ctttttagca tgttgaacag gtatattgcc
    12361 atgctttcat tattttattt ttttgcctta tactggacta tatttgcagt ttcctaatat
    12421 atttggctca tatttaaata ccattatcta aaagcactat tgctgtacga gaggcaatag
    12481 gcattttaga agaaggagga gttgagctaa agtatacatc ataatctctt aagttttctt
    12541 tttttgtgtt tcctccaaac tctaccatat cagtatttaa tatcgttttg tagtttgtat
    12601 tttgaaactt tgaaatatca actgattgta ttagattagg gtggaaatta aatataaata
    12661 aatatttgcc cttactgtac accaaaatct ttttatcatt atccatatgt ataagttcat
    12721 gactattttg gataaaaata tcactgtatt ttgtaagttc gagcatagat ttatcaaaat
    12781 ttaataattg tttatacttt aagttgttat cttctgaaag gctccattgt cttctggcat
    12841 acttatagct ccaattgttg ccttctcgtg gaaaatcaat ccactcagga tgtccaaatt
    12901 catttcccat aaaatttaga tatccttcac ctgctagaga aaaagttatt aatttaatta
    12961 atttaataag agatattgct ctattgataa catggttatt tgagtttatt tccatattcc
    13021 agtacatttc cttgtcagct agccaaaaaa taattgtttt atcgccaact aatgcttggt
    13081 catgggattc acaatatcct atattttttt ctccaggtcg tctagtagta agttcatacc
    13141 acattttgcc caaatcccag tcttcatcag ataagttaga gatagttttt atccaaaaat
    13201 caggtactcc cattgcgagt ctatagtcaa atccaattcc gccgtctttt atagggatac
    13261 acataccagg cattccactc atatcttctg caatacttat cgaattaggt ttaatttctt
    13321 tcattaattc atttgcaaat tggagatatg ttatagcttc aatatctgta ttcatactaa
    13381 aatatttttc ataactatca aatgaaactc caagaccatg gttatgatat aacattgaag
    13441 ttacaccatc aaacctaaag ccatcaaaat ggtactcatt taaccaaaat tttatatttg
    13501 aaagcaaaaa atgtattact tcaggtttac catagttaaa aagttttgta ccccaagcag
    13561 gatgatttcc ctttgaacct gagtggaaaa attgatgttc acttccatca aactcattta
    13621 ttccttctaa agtattcttt acagcatgag aatgaactaa atctaataaa acagcaatcc
    13681 ccattgaatg agcagtgttt atgaggtttt ttaagtcttc aggagtacca aacctagatg
    13741 agattgcata aaagtttgaa acttggtatc caaaagatgc ataatatgga tgttccataa
    13801 tagccataag ctgaatggta ttgtatccag ctttttttat cttaggaagt attttttcag
    13861 taaattcatt ataagttcca atagattcgc tttcagtaga catacctata tgacattcat
    13921 agattagtgg agaagttatg ttttttaagt caaaatcatt gtcagtccag ataaatggag
    13981 tttttggttg ccatatttgt ccattaaatc caccaaaatc tttttgaatt actcttttta
    14041 tatataatgg tatacggtca aatgtttttc catttgcagt aacttgtacc tttacctctg
    14101 atttgtgagg taagctattt tgaccaggaa taaatatttc ccaattacca gacccaattt
    14161 ttcttagagg atgagatttt ctattccaat tattaaagtc accaattagt gacaaactat
    14221 ctgcatttgg agcccattcc ctatagaaga atccatcatt tgtctgatga aatccatagt
    14281 aaagatgagc atttgcaaaa gaactaaaat ctttatagtt tttcaatgtt tttgatttca
    14341 tatcctcata atgtttcatt cgctttttta tatcagattc aaatggttgt aggtatacat
    14401 caatatctaa aattttaaat cttttttgac ttttcattgt atttctcctc cataataaat
    14461 attacatcta aaaaataata tttatctcat actatcttct ctgtataatt gatatatatt
    14521 atataatcct aataaaaagc ttaagccacc aatgattaat ttcataattt acctccctag
    14581 tatttatact tatatatcgt attcatcaaa atttaaaagt ttgtttaaaa ctcttttaat
    14641 aatatcataa tcaaagcctt tataggaaag atgttgagaa attttttgat atattttctt
    14701 tttatcttct ttctttactc tttcatacct tttttttgct agatacatag cattttcaaa
    14761 ttcagtgtcc ttgtctaatt cagatactgc ttcattgatt gtacttctat tgataccttt
    14821 attatataaa ttctgcttaa ttctgttttt gccacattta tttaggttaa catttgtgtt
    14881 aacaattttt tgagctaata cactgtcatt tacgagatta taacttttaa gaaaatctat
    14941 gaccctctca attatatttt catcaaattc tgatgatagt ttttctttta attttttttc
    15001 agattggtca gcatgagata aaatatttaa tgctttattt ttagctttta tatacatttc
    15061 atcatctaaa ataggtttta attcttcttc atttatttca ttcccttttt ttaaattaaa
    15121 tgtataaacc agttctttaa atatagcaat aaaaaattta tcatctacat aaatattaac
    15181 tctatcatca tttctttttt gttgttctat ttttgtaatt atacccataa gtcctccttt
    15241 tttatatgta attatattaa caaaagcaac taataaaatt taacaaaaaa cttatatttg
    15301 tatgttatat tttatcaaaa ttaagacaaa atagtaaaaa gttattttta ttaaaaagta
    15361 aagcagaatc atgttaaata attatttttt aaagtgtttt ttagcatata aatataatta
    15421 tatacccaaa aaaacaaatt ataagcttta agaaataaaa aataatggta caatagtatt
    15481 ataacattaa aaggagacaa aatatgagaa gtgaaaatct agttaagatg gcagagttga
    15541 aaaatactga aaaactagag aaatttaatc gccataaagg caaaatcaaa ataggtatat
    15601 tgggaggtac ttttgaccct atacactatg ctcatttagc aacagcagaa tttattagag
    15661 acaagtatga cattgacaaa ataatcttta taccatcagg aaatccacca cataaattat
    15721 gtatcactac agataagtat gatagatata atatgacact tctagcaact gaaagcaacg
    15781 aagatttttt agtttcaaaa gtagaaatcg aaagaaagaa aaggacttat acaatagata
    15841 cattaaaata cttaaagaaa aaatacaaaa atgcagacat ttattttata acaggtgcag
    15901 atgcaatttg tagtgttgaa gagtggaaag atgtaaagaa aaactttgag ttagctactt
    15961 ttatagccgc aactagacca ggaattagtt tgttaaggtc acaagagact atagaaaaac
    16021 taactaagaa gtataatgct gacattataa cagtttatgt tccatcactg gatatatcgt
    16081 ccacatatat aagggaacag ctaaatgaag gcaagtcaat acgttactta gtacccgaaa
    16141 atgtagaaaa ttatttatat gaaaataaac tatatcaata tggagatgat taaatgaact
    16201 tagaaaatat taaccaaaga ctaaatcaaa tgctaccagt ggggaggctt agccattcaa
    16261 aaaatgttgc aaaatgtgct gaaaaattat gtgagattta tggatgtgac aaagaaaaag
    16321 cttacttagc aggaatgatt catgattgtg caaagtattt aagtgacaag gaaatagagg
    16381 attatgtaaa taagtatgaa atatatttag acccaataga ggatggaaat cgttccttgt
    16441 ctcatagtgt aattggggcg tacatatgcg agtatgaatt tgaagtggaa gatgaagata
    16501 ttataaatgc aatcaaatat catacaacag ggagagagga catgtctctt ttagaaaaaa
    16561 taatttacat agctgattta attgaagaag gaagaaaatt cccagttgta gatacattaa
    16621 gagaattagc atatggaggg aagcttgatg aagcactttt aacttctttt aataatactt
    16681 taatgtttgt catcaataaa aaagaagaga tacatccaag aacagttatg gcaagaaact
    16741 atctaattaa agaaaagtta ttataaaaaa gctatataaa ggcaaacata ttaataaatt
    16801 taaatttgac tatgttacaa aatcgtatta atatgttaca ataactaagt gtattttaat
    16861 taggaaagtg aggaattttt atatgacagt ggaacaaatg acaaaaatag catatgatgc
    16921 catagaagat aaactgggtc aagatacagt tataataaat ataggaaaag tttctagttt
    16981 gtgtgattat tttattatta ctacagcatc ttctcagaga caggtaaaag ctattgctga
    17041 caatgtggaa gatgaattag caaaactagg attagagcca agaggtaaag aaggacaagg
    17101 aactcaaact tgggtactcc ttgattatgg agacataatg gttcatgtat ttaatgagga
    17161 aaatagaggg ttttataatt tagaaaaatt atggaaagat gcaccttata tagatattga
    17221 cacattagca taaacatgat aatatatgtt attataatta gaa
//
LOCUS       DAEOSP010000065        17152 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.065, whole genome shotgun sequence.
ACCESSION   DAEOSP010000065 DAEOSP010000000
VERSION     DAEOSP010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17152
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.065"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            63..788
                     /locus_tag="KRH54_001791"
     CDS             63..788
                     /locus_tag="KRH54_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBG8579455.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
     gene            complement(916..2112)
                     /locus_tag="KRH54_001792"
     CDS             complement(916..2112)
                     /locus_tag="KRH54_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8579456.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDNKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(2115..3140)
                     /locus_tag="KRH54_001793"
     CDS             complement(2115..3140)
                     /locus_tag="KRH54_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBG8579457.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KLIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            complement(3212..4342)
                     /locus_tag="KRH54_001794"
     CDS             complement(3212..4342)
                     /locus_tag="KRH54_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG8579458.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            complement(4936..5331)
                     /gene="tnpA"
                     /locus_tag="KRH54_001795"
     CDS             complement(4936..5331)
                     /gene="tnpA"
                     /locus_tag="KRH54_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBG8579459.1"
                     /translation="MKLDSNNHSVFLLHYHLVMCIKYRRKVIDDSISDRLKDIFVDIA
                     PNYGVVLIEWNHYVDHVHLLFKAKPNTEISKLLNAYKSASSRRIKNEFPSIRKYLWKE
                     YFWSKSYCLVTTGGATLEDLEQYIENQEE"
     gene            complement(5496..6503)
                     /locus_tag="KRH54_001796"
     CDS             complement(5496..6503)
                     /locus_tag="KRH54_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG8579460.1"
                     /translation="MRLREYIGDKVVFLGTNLIIFLIASGIMLYSKINVVLIFMLFCI
                     WFLPLFTYMLIDYLQYKKYFNSIDNILDALDKKYLLPEVIDDVNFVTGEKINDILKVI
                     SRNMHENIKYYNDNQEEYKEYIETWVHEIKTPIASSKLIIENNSNEITKKIDTQLDKV
                     ENFVEQVLYYSKIDNVNKDYIIKKLNLEEVVNEVIKRNYRDFINKNIKLELGEINKTV
                     YSDSKWVEFILNQIVVNSIKYIKSKDGLIKISAKRVENSIVLTIEDNGVGIEERDINK
                     VFEKGFTGENGRVFGKSTGIGLYLCKKLCNKLGLGLILESKVNVGTVIKVSFPIDYNL
                     F"
     gene            complement(6500..7171)
                     /locus_tag="KRH54_001797"
     CDS             complement(6500..7171)
                     /locus_tag="KRH54_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005210744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8579461.1"
                     /translation="MEKIIIVEDEEVIRDELKSFLERYGYEVMAPTEFDNIVEFILSE
                     KPNLVLLDINLPIYDGYYICRVLRKESNIPIIIVTSRNTDMDELMSMNLGADDFVTKP
                     YKTQILLARIVALLKRSGLNSNVNNTLIFKGIELNLFNATLRYKDKEIELTKNEFKIL
                     SYLVENQGSIVSRDSFMEYLWHTNWFVDDRTLTVNITRLRKKLDEIGIENLIETRRGL
                     GYIIS"
     gene            complement(7248..9242)
                     /locus_tag="KRH54_001798"
     CDS             complement(7248..9242)
                     /locus_tag="KRH54_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8579462.1"
                     /translation="MYSKIAIKNVRKSFKDYSIYFLTLTLAICIFYSFNSIESQKALM
                     EMNASTKSHVEMLKNMISYMSVFVSVILCSLILYANNFLVKKRNKELGIYMTLGMEKV
                     KISKILVFETIIVGIVSLVSGLILGLLVSQGLSILLLKLFEFNMSEYNFVISISSIIK
                     TIVYFGIMFLLVMIFNTYIISKYKIIDLLTIGRKTEDIKFKSPFVYTITFILCIISLI
                     IAYSMVLKVGLNPKNPKFIMSIVLGVIGTILFFYSLAGFALYIVKKNKNIYFKGLNIF
                     VVKQINSKINTNFISISIICLMLFLTIGILSTGFGFKSALNAGLKGATPFDASATMIV
                     HEKCKVKNIKESFEKVGIKFNKGDKVAYYDEYINGNKLSDVMQGQYSGRVDFEVAYIK
                     SSQYNEIRKLRGEKSITLSKDEVLITSNATEVVPSIEEYMKNNKIIKIDNKAYNIKNK
                     KVIKDNLRTDFTETNALTVVVNDKLCDSMEISSSIVNINFASDNNEKSQENFDKRLKS
                     KYSSTSFYEVGTVSGGSRNAMYNDNNGMTSTILFVGIYLGIVFLISSMAVLALQQLSE
                     ASDSIDRYVSLKKLGASKNSISKTIFTQTLMYFSIPVGLALVHSVVGIQVANNFISRL
                     NKPDIGASSLMTVGILMLVYAVYFYTTYQGYKNIVIKKLN"
     gene            complement(9232..9999)
                     /locus_tag="KRH54_001799"
     CDS             complement(9232..9999)
                     /locus_tag="KRH54_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8579463.1"
                     /translation="MKNILSVEKIEKYYGNKDNITKAIDNISFKVDEGEFVGIMGPSG
                     SGKTTLLNCISTIDNVTIGKIIINNNDITRLKSKNLDKFRQNELGFIFQDFNLLDTLT
                     AYENIALALTIKGEKPSKIDSKIRDVATYLEIEKVIDKYPYQMSGGQKQRVASARAIV
                     TNPSLILADEPTGALDSKSARLLLERFESLNQDLKATILMVTHDAFTASYAHRILFIK
                     DGKIFTELVRGADSRKEFFNRIIEVTTLIGGDDNDVF"
     gene            complement(10254..11099)
                     /locus_tag="KRH54_001800"
     CDS             complement(10254..11099)
                     /locus_tag="KRH54_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003485307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8579464.1"
                     /translation="MYSKIAIKNVRKSFKDYSIYFLTLTLVICIFYSFNSMESQKALM
                     EMSTSKKPDLEMFKNMISYISVFVSAILCTLILYINNLLIKKRNKKLGIYMTLGMGKI
                     EMSKILVFETIIVGIVSLVSGLILGLLVSQGLLILLLKLFEFNMSEYSFVISIPAIIK
                     TIVYFGIIFLLVMIFNIYLISKYKMIDLLIVERKTEDIKFKNPFVYMITFVSCFIFLF
                     TVYNTTNHIIINLIDDKQLNSKSKGVEINEFTYQDWQQFCDIKYYGKKGLISMTLKEY
                     IEKYK"
     gene            complement(11089..11562)
                     /locus_tag="KRH54_001801"
                     /pseudo
     CDS             complement(11089..11562)
                     /locus_tag="KRH54_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521021.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12268..13161)
                     /locus_tag="KRH54_001802"
     CDS             complement(12268..13161)
                     /locus_tag="KRH54_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBG8579465.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     gene            complement(13241..14143)
                     /locus_tag="KRH54_001803"
     CDS             complement(13241..14143)
                     /locus_tag="KRH54_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG8579466.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            complement(14193..15353)
                     /locus_tag="KRH54_001804"
     CDS             complement(14193..15353)
                     /locus_tag="KRH54_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG8579467.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            complement(15509..15814)
                     /locus_tag="KRH54_001805"
     CDS             complement(15509..15814)
                     /locus_tag="KRH54_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBG8579468.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAKGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            complement(15912..16886)
                     /locus_tag="KRH54_001806"
     CDS             complement(15912..16886)
                     /locus_tag="KRH54_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBG8579469.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
BASE COUNT         5659 a         2926 c         1738 g         6829 t
ORIGIN      
        1 aattaaatat atgactattt tgctactaca tatataaaaa taaatttatg aaggtgattt
       61 taatgaaaac tatttctaaa caagaattag aaaaaacact tgagaaacat tatttatggc
      121 ttaaaaacaa taatgctgga gaaaaggcag atttaaaaga attaagatta gaaaatatgg
      181 acttaagagg atactgctta agtaatattg acttttcgtg gtctgacctt atcaatttaa
      241 atttagaaaa agcagattta gaaaactcta tttttaataa ttcttatctt tcggattgtt
      301 cattaaaaaa ttctatatta aaaaatgcag acttaagtgg tgcaagtttt agattttgtg
      361 acttatctaa ctcagatatt agaggagcta atttagaaaa ttcaaattta gaacatgcta
      421 ctttaaatgg agtaatatca gatgaaagta cccgtttttt taaattgtat tgtcctgaaa
      481 caggagcatt tataggctat aaaaaatgtt ttaattttag aatggtacaa ttattaatac
      541 ccagcgacgc aaaaagagtg tctgctacat caaatgcttg tagatgtgat aaggcaaaag
      601 tactgtcaat tactagtata gatggtaaag aatcttttaa aagtgctcgt gcatatgctg
      661 atgaaaattt tatatataga gtaggagaaa cgattgtagc tgaagatttt aatgaaaata
      721 gatggaaaga atcgactaca gggattcact tttttttaac tagagaggaa gctattggtt
      781 atctataaaa tgctaaagtt ttataaatat ataaatacat aaacaaaata acaaaagagt
      841 atagattata atttcaaaaa aattataatc tatactcttt tttctatagc acataagtac
      901 attttatttc ataaattata ataaattatt tatatctgca ctattgatta tttgagaaac
      961 tttatctata tgttctatct tattatccaa actgtcataa tacttcttac tctcatctct
     1021 atttctaaaa gaataaactt ttttatccaa ttttaaagcc tcttttataa tatctatatt
     1081 cctcttattt gtctctccta cactaaatcc tgtatctatt ataatcttgg aatcatttaa
     1141 atttctaata gccaaatcga aactctcatc acttataggc tcaaatggat tttctgtaaa
     1201 catctttatc cccatagttc taccaatttc ataatcaata tcattttcat gtattattcc
     1261 agaatacaga cctatacctt ttttagtaaa agctctataa attggtgttg ctttaccccc
     1321 accaccaatt ataaaaactt catttttaga tttattacta atttctactg aacctaaaag
     1381 actattaaag tttttatcat ccaattcata aagcgaattt ataatatcct catctactac
     1441 atcttcaggc tgaccataag ctatgacttt attatttttt ataagagcaa ctttatcaca
     1501 actttttaat gctatgtcta tttcatgcaa tgaaagtatt actgatattg acttttcttt
     1561 acttaatttt ttcagtatgt ttatgagttc caatctatgt tttatatcca agtgagttgt
     1621 aggctcatct aatataatta tttctggttc ttgaacaaga gcccttgcaa ccaacacctt
     1681 ttgtttttct ccatcactta gttcatcaaa atatcttttc tttaaatgta aggcatctac
     1741 agactccaat gcttcatcta ccattattaa atctttctta gatagactcc caaaaaatcc
     1801 tgtatatgga taccttccta tattgactat atcttgtaca gtcattaaat cacctaataa
     1861 tctatttgta agtactaaag ccattttttt tgataatgct tttcttgata tacaatttat
     1921 gtttacacca ttcaaatata tttcaccttt tattggtgat ataagtttag ataaggacct
     1981 taatagggta gtttttccag ctccattact tcccaaaaga catagtattt ctccattttt
     2041 tacttctaca tttatattac ttataacaac tttgttattg tatcctacac ttaaattatt
     2101 agttttcaac atgtctaagc cctatccttc cttttcacca ataacattac tacaattgga
     2161 gctcctaata aagatgttat cgtacttatt ggtaattcta ctggtgataa aattactctt
     2221 gatattaaat cacaaagagc tgtaactata ctgcctaaaa atattactac tggtatcaaa
     2281 actctattat ctgatgtttt aaatatcaat tttgaaattt gtggcactgc aagtccaata
     2341 aaccctatag gtcctgcaaa tgctgatact attgctgcta atatactaga tataaatact
     2401 atcaaaactc taaaaaatct tatatttatg ccaatacttt ttgcatactc ttctcctaat
     2461 aaaaaagcat ttaaaggttt tattatgaat acagtcatca aaagacctaa tataccaact
     2521 attataagta tttctacctt ttcccatgta aatccagaaa aacttcccat agaccacata
     2581 gtaaattctt ttaatttatc actatttgca aatgattgta acacatttgt tataccacta
     2641 catacatatc cggtcataat tcctattatt aaaagagttg ttatattctt taccttattt
     2701 gcaaataaaa tgactataga cattacaatt aaagctccta taaaagctgc tacaaatata
     2761 ctcattgaat ttacactctc aaatcccaat gtgtatcctc ccaaaatcat gagagctact
     2821 gataaagtag aacctgaaga tactcctaat atatacggtt caactattgg gtttttaaaa
     2881 aatatctgta ataaaatacc tgatactgct aggcatgctc cacctaccat ggttgcaagt
     2941 actctaggga ttcttatatt gtatattatt ttataatgtt ctgtattatc catacctctt
     3001 aatacaacat ctattacttc tttaaattca atttttacag agccaaatgc tacacacact
     3061 aaaaaagtaa gtatcatact tataattaat actataaata ttgttgtatt ccttattctc
     3121 attttattga taaaatccat ttgtatactc ctcttagcaa agctatatta actaaaatct
     3181 ataaaaccta tctttttgtg tcctctctgt actatttatc ataatttata taatgtttga
     3241 ttttttcacc tgtagtacct gggtggaaca tctcaactaa atctactatt aattcgtccg
     3301 tcttatctac tgattgataa taatctggag caaacaccca taggtttccg ttttttacaa
     3361 catccaaatc agccaataca ggtgcactag attttataga atctaaattt ggtgcataat
     3421 ctgaacttga tgaatataca aatatatctg catcttttgc aacttcatgc aattgttcaa
     3481 tacttactcg cccttcattt gaatctattg aagcacatgc atttattcca ccagccattt
     3541 ttatcatatt atccacatat gaatctttag gagctatata agcacttcct tcatatacag
     3601 atgcccatac aactttaggt ttagctttgt tttttatttt tttacttact tcttctacct
     3661 tttgtacagt tttatttagt tcactagtag ccatatcttc tttatcataa aatgcagcta
     3721 ttaaacttgt ccactccatt cttccaaatg gattttgctc tttatactca ttgtctacaa
     3781 cataattaag ccctaattct tttaatttgt caataaattt tgtatcagcc tctgataaac
     3841 cactactcac tatagttaag tcaggattta agtctactat tttttcataa tctggtgcta
     3901 aatttgaacc aacgtaatgt atttttccat ctttcattaa atctttaatt tctttaatag
     3961 tccagtactg ctcttctgtt gttacggctt ttatactatc cagttcatct attgttctca
     4021 gagaacaagc ctgtgttgta gatgctatta atacattgtc cacaggcgtt ctaactatat
     4081 tagcattttt atatttttca ggaattttaa tcttctttgg gattaaaatc agttctcgat
     4141 tatctccatc agttactttt ttaattccat cttctaaatc ttcaacacta aatccactag
     4201 catattttaa ttctaatttc ttaccagtac tagcttgttt attctctttg tcacttttac
     4261 tacaccctat aagacctcct accataaaaa ttaccatagc aattaaaaaa aactgcttta
     4321 aaacatttga tttaaatttc atctctttca ctccaatatc ttatttttta tgagtggcta
     4381 aaaaacttta ataagatata ccttttaaag tatatcttat taaagttata ttaaaccatc
     4441 tttaacaagt ataacttttt aataaaatta tagtgaattt tgatttcaca tgcaattaac
     4501 tacttataca cttaactacc tacgacaacg tttgaagtgg catcttcttt gtcaaaatag
     4561 ctattgttat tagtatctcc aagctaatag gatagttccc aacacttacg attagcatct
     4621 gagtcactac gttggcaaac taatagaaat ataacattta tttgatgatg ctttagatac
     4681 agtacaactc tatcaaattg cctatgttgt tttatcctaa tcattgattt taatttaagc
     4741 agttttatat acccatttct atatgcacaa tatcttttga gtaacgtgtt gacaaactat
     4801 tttggttagt agtatagcta tgatagttaa ttttctttga tagataagtc cgttacatcc
     4861 aaaattctta ggaaaatatt ccttttattc actgtttgta taaacctgta cttataagct
     4921 cttaacatat tatcactatt cttcttgatt ttctatgtat tgttccaaat cttccaatgt
     4981 tgcaccacca gttgttacta aacaataact tttactccaa aagtattctt tccataagta
     5041 tttgcggata cttggaaact cattctttat ccttctgctt gatgcagatt tataggcatt
     5101 taataacttg gaaatttctg tattaggctt tgctttgaat aataagtgaa catggtcaac
     5161 ataatgattc cattctatca acaccacccc ataatttggt gcaatatcta caaatatatc
     5221 ttttagtcta tcagatatag aatcatctat tacttttctt ctatatttta tacacataac
     5281 taaatgataa tgcaataaga agactgaatg attattacta tctaatttca ttataattac
     5341 ttcccttcag cttttactac taaattaatg atataatacg tttttatgaa aatcaagtaa
     5401 aaaaaagaac ctatcaatta tgcttacatt cttaactttc ttaaataaat acaaaaaact
     5461 gacaactaaa aattgtcagt ttttataact atacactaaa ataaattgta atctataggg
     5521 aatgatacct ttatgactgt accaacattt acttttgatt ctaatattaa acctaaacct
     5581 aacttattac ataacttttt acatagatat aatcctattc cagtactttt tccaaatact
     5641 cttccattct ctcctgtaaa ccctttttca aataccttat taatatctct ctcttctata
     5701 cctactccat tatcttctat agttaacact attgagtttt ctaccctttt tgcacttatt
     5761 tttattaaac catcttttga ctttatatat tttatagaat ttaccacaat ttgatttaat
     5821 ataaactcaa cccatttcga gtcactatat acagtcttat taatttcacc aagttctaat
     5881 tttatatttt tgtttataaa gtctctatag tttcttttga taacttcatt tacaacttct
     5941 tcaaggttta gcttttttat tatataatct ttatttacat tatcaatttt tgaataatat
     6001 aatacttgtt ccacaaaatt ttcaacttta tctagttgag tatctatctt tttagtaatc
     6061 tcattactat tattttctat aataagttta ctagatgcta taggcgtttt tatttcatga
     6121 acccaagttt caatatattc tttatattct tcttgattat cattataata tttaatattt
     6181 tcgtgcatgt ttctacttat tacttttaaa atatcattta ttttttctcc agttacaaaa
     6241 tttacatcat caataacttc tggcaacaaa tattttttat ctaaagcatc taaaatatta
     6301 tctatagaat taaagtactt cttatattgt aaataatcaa ttaacatata agtaaataat
     6361 ggtaaaaacc atatacaaaa taacataaat attaaaacga catttatttt actataaagc
     6421 atgatacctg atgcaattaa aaatataatt aaattagtac ctagaaaaac aactttatca
     6481 cctatatact ctcttaatct catgatatta tataccctaa accacgtctt gtctctatca
     6541 aattttctat gcctatttca tctagctttt tacgtagcct tgttatattg acagtaagag
     6601 ttctatcgtc tacaaaccaa tttgtgtgcc atagatactc cataaatgaa tctctagata
     6661 ctattgaacc ttgattctcc actaaataag atagtatttt aaattcattt ttagttaact
     6721 ctatttcttt atctttatat ctgagtgttg cattaaataa atttaattca atacccttaa
     6781 atattaaagt attatttaca ttcgaattta atccacttct ttttaacaat gcaacaatcc
     6841 ttgctaaaag tatttgtgtt ttatatggct ttgtcacaaa atcatctgct cctaaattca
     6901 tgctcattag ttcatccata tcagtgtttc tacttgttac aattattatt ggtatattcg
     6961 attctttacg taatactcta catatataat atccatcata tattggcaag tttatatcaa
     7021 gtaatactaa attaggtttt tcacttaata taaactctac tatattatca aattcagtag
     7081 gagccataac ttcatatcca tacctctcta aaaagctttt taattcatct ctgattactt
     7141 cttcatcttc cactataatt attttttcca ttaatttttc accctttatt ttcaaaaaat
     7201 ctatttacag tatgattttt actgtaaaca gattttagtt agttgtatta attaagcttt
     7261 tttataacta tatttttata cccttgataa gttgtataaa aatacactgc atatactaac
     7321 attaaaattc ctactgtcat aagtgatgaa gctcctatat ctggcttatt aagccttgat
     7381 ataaaattat tagccacttg tattcctact actgagtgaa ctaaagctag tccaacaggt
     7441 atgctaaagt acattagagt ttgtgtaaat atagttttac ttatactatt tttacttgct
     7501 cctagttttt ttaaggatac atatctatct atactatcac tagcctcact taattgctga
     7561 agtgcaagga ctgccatact acttattaaa aatactattc ctaaatagat tccaacaaat
     7621 agtattgtag aagtcatacc attattatca ttatacatag catttcttga tccacctgat
     7681 acagttccaa cttcataaaa acttgtagaa ctatactttg attttagcct tttatcaaaa
     7741 ttttcttgtg acttttcatt attatctgaa gcgaagttta tatttactat acttgaagat
     7801 atctccatgc tatcacacaa tttgtcattt acaactacag taagagcatt tgtttctgta
     7861 aaatcagttc ttaaattatc ttttattact tttttatttt ttatattata tgctttatta
     7921 tctattttta taattttatt atttttcatg tattcttcta tcgaaggtac tacctctgta
     7981 gcattagatg ttattaatac ttcatctttt gataaagtta ttgacttttc acctcgtaac
     8041 tttcttattt cattatactg tgaagatttt atatatgcta cttcaaagtc cactctacca
     8101 gaatattgtc cttgcataac atctgaaagt ttattaccat ttatatattc atcatagtaa
     8161 gcaactttat cacctttatt aaacttaatt ccaacctttt caaaagattc ttttatattc
     8221 ttcactttac atttttcatg tacaatcatg gttgcacttg catcaaatgg agttgcacct
     8281 tttagtcctg catttaaagc tgatttaaaa ccaaaccctg ttgatagtat tcctattgtt
     8341 aaaaataaca ttaaacaaat aatagatatt gatataaagt ttgtattaat cttactattt
     8401 atttgcttta ctacaaatat gtttaatccc ttaaagtaaa tgttcttatt cttcttaaca
     8461 atatataatg caaagcctgc taaactatag aagaataata tagttccaat tacacctaat
     8521 actattgaca taataaactt aggattttta ggatttaatc caacttttaa aaccatgcta
     8581 tatgcaataa ttaaggaaat aatgcataat ataaatgtaa ttgtatatac aaatggactt
     8641 ttaaatttta tatcctctgt ttttcttcct attgttaata aatctattat tttatattta
     8701 gaaattatat aagtattgaa tatcatcaca agtaaaaaca ttattccaaa atagacaata
     8761 gttttaataa ttgatgatat cgatattaca aaattgtatt cactcatatt aaattcaaat
     8821 aactttaata ataatattga taacccttga gatactaata acccaagtat tagtccactt
     8881 actaaagaaa ctattccaac tattatagtt tcaaaaacta atattttcga tattttaact
     8941 ttttccatcc ctaaagtcat ataaattcca agttccttat tccttttttt gactaagaag
     9001 ttattagcat aaagaattaa actgcataaa ataactgata caaatacaga catgtatgaa
     9061 atcatattct taagcatttc tacatgagat tttgttgaag cattcatttc cattaaagct
     9121 ttttgagatt ctatagagtt aaaactatag aaaatacaaa ttgctaatgt taaagtcaaa
     9181 aaatatatgc tataatcctt aaagcttttt ctaacatttt taattgctat tttagaatac
     9241 atcgttatca tctcctccta ttagagttgt tacttcaatt attctattaa aaaactcttt
     9301 tcttgaatca gctcctctta ctaattctgt aaatattttt ccgtctttta taaataggat
     9361 tctatgggca taactagctg taaaagcatc atgagttacc attaaaattg ttgcttttaa
     9421 gtcttgattt agactttcaa acctctcaag taataatctt gctgattttg agtcaagtgc
     9481 tcctgtaggt tcatctgcca aaatcaatga tggattagta actattgctc tagctgatgc
     9541 tactctttgc ttttgtcctc ctgacatttg ataaggatat ttatctatca ctttttctat
     9601 ttctaagtat gtagctacat ctcttatttt tgaatcaatt ttagatggtt tttctccttt
     9661 aattgttaat gccaatgcta tattttcata agctgttaaa gtatctaaaa gattaaaatc
     9721 ttggaatata aatcctaatt cattttgtct gaacttatct aagtttttag attttaatct
     9781 tgtaatatca ttattattta ttattatttt acctattgtt acattgtcta ttgttgatat
     9841 acagttaaga agtgttgtct tcccactacc acttggaccc attatcccaa caaattctcc
     9901 ttcatcaact ttaaagctta tattatctat tgcttttgta atattatctt tgtttccata
     9961 atatttttct attttttcaa cacttaatat attcttcatg ttctatttct cctttaaatt
    10021 aattttctta tatctctatt gtaatttttt atttaatttt tttaaattga tttacatttc
    10081 attaatgttt catttttgta gtaattatat attattccct aataattact aatataaaat
    10141 actcaagacc agcattcttt cttttatact ttgctagagt aacaaaattt actcaattct
    10201 agaaaacaca aaaatataga gggcaatgcc ttccatctaa aatttatttt attctacttg
    10261 tatttttcta tatattcttt taaagtcatt gatattaaac ctttctttcc ataatacttt
    10321 atatcacaaa attgttgcca atcttgatat gtaaattcat ttatttctac acctttagac
    10381 ttggagttta attgtttatc atctattaga ttaattatga tatgatttgt agtgttatat
    10441 acagtaaata agaaaataaa gcatgataca aatgtaatca tatatacaaa tggattttta
    10501 aattttatat cctctgtttt tctttccaca attaataaat ctatcatttt gtatttagaa
    10561 attaaataga tattaaatat cattacaagt aaaaatatta ttccaaaata gacaatagtt
    10621 ttaataattg ctggtattga tattacaaag ctatattcac tcatattaaa ttcaaataac
    10681 tttaataata gtattaataa cccttgagat actaataatc caagtattag cccacttacc
    10741 aaagagacta ttccaactat tatagtttca aaaactaata ttttcgacat ttcaattttt
    10801 cccattccta aagtcatata aattccaagt ttcttatttc tttttttgat taataagtta
    10861 ttaatataaa gaattaaagt acataaaata gctgatacga acacagatat atatgaaatc
    10921 atattcttaa acatttctaa atcaggcttt tttgaagtac tcatttccat taaagctttt
    10981 tgagattcca tagagttaaa actatagaaa atacaaatta ctaatgttaa agttaaaaaa
    11041 tatatactat aatccttaaa gctttttcta acatttttaa tcgctatttt agaatacatt
    11101 gttatcatct cctctgatta tagctatttc ttcaattatt ctattaaaag actcttttat
    11161 tgaatcagtt cgtcttgcta actctataaa tatttttctg tattttataa ataagtttct
    11221 atgagtataa ctagctataa aagcatcatt agttagcatt aaaattgttg tttttaaatc
    11281 ttgatttaaa ctttcaaaac tctcaagtaa taatcttgat gcttttgaat caaatttata
    11341 ttgttttccc tttaattgtt aatgtcaatg ctatattttc ataagctgtt aaagtatata
    11401 aaagattaaa attttggaat ataaatccta attcattttg tctaaattta tctaagtttt
    11461 tgaattttaa tcttgtaata tcattattat ctattgcttt tgtaatatta tctttgtttt
    11521 cataatactt ttccattttt caacacttaa tacatttttc atgccttatt tctcctttaa
    11581 atttcttata tatctattgt aatttttaat ttaatttttt taaattgatt tacatttcat
    11641 taatatttca cttttgtagt aattgtatat tgctacctat aattactaat ataaaatgtt
    11701 caagaccaac agattttctt ttgtgcttta ctagaatcgt aaaatttacc caattctaga
    11761 aaaacacaaa aaatggaagg caatgccttc cctctaaaat ttattttatt ctaattgtat
    11821 ttttctatat gttctttcaa aatcattggt attaaatcat tcttactata gtgctttgta
    11881 tcacaaaatt attgccaatc ttgatatgta aattcattta tctttatact tttaagcttg
    11941 aagtctaagt gtttatcatt tattagatta attattacat gatttgtagt agtttatatg
    12001 tggctcttat tgttaactta tacttatcta aaaatgtttg taaaactcca agattatttt
    12061 taatagataa aggctattgc tttaatattt gtgctaactt ttaaactaat ctgctatctt
    12121 agtattattt aatttttatt aacataattg taatttattg ttgaccactt ttcaaagttg
    12181 atttaacata tataaaaaga gtgtttaaaa tgattataaa aatcagttta aacactcttt
    12241 ttttgctcat atatcttact tagtaagtta acatatattt aattcattca tcaatttttt
    12301 aatttcacat acttgttttt catttggttt tacaaatgga gcagtcatat aatcacttat
    12361 ttgtaaccct ctgatattca tagctttttt cattgctggt ataaatggat tggttattaa
    12421 gtaaaaatcc attgctttat taatatattt ttgaatttct ataaccttct ccatatctct
    12481 attattgtaa gcttcagccc atttggagca aacttctgga attaaatttg ataacccccc
    12541 tatacaacct gctcccccag acattagatt atgtataaag ttttcatcat aaccactata
    12601 aaccttaaat tctgggaact ctattttcat agtatttatt aaatctctgg tatgtcccat
    12661 caaagttact gtatccttat atccaactat gtttttatgt tttcttgcaa gtcttaatgt
    12721 aagttctgga cttaaatcat aaccagttct atctggataa ttgtataaat atatttcagc
    12781 ctctgtattc ttagctattt cactataaaa taactcaata ctatcatcat caagagagaa
    12841 ataataaggg cttattatca taacaccatc tgctccacta ttatgtgcat agtttgaaag
    12901 ttctatagat tcatttatac acatacgact tgtacctata aacactttta ctctcttatt
    12961 tacatgacta gttactaaat ctataagttc tttttgcata tccatagaca tattaaagaa
    13021 ctctccagta cttcccatta tgactagtcc atctacacct ccatctataa gatggtcata
    13081 cactttttta ttaccttctt tatctaaatt tccttcttca tcaaatattg taacaactgg
    13141 tgtcaaaaat tctgctttca taatttaaac tcctaaaatt tattttaata tgttcattaa
    13201 tacatcagat ttagctttaa tattaattat attaaaacag ttacttaatg taatttttta
    13261 aaattccaat actctcaatc tcacattcaa ctacatctcc actactcata tatcgaggag
    13321 gtgaaaatcc catccctact ccagaaggtg ttccagtagc tataatgtct ccagcctcta
    13381 aggtcatacc ttttgaaagt tctgaaacta tgtctgaaac gtctgatatg aataatcctg
    13441 tattagaact ttgtctttct tcaccattta ccctactaca tactttaagt tttataggaa
    13501 aaggaactgc acttttatgt aaaatacatg gacctaaaga ggtaaatgta tctaatcctt
    13561 ttcctctaaa ccattgaaca tgttcttttt gcaactttct agcagaaata tcatttataa
    13621 cagtgtaacc aaagatataa tcttctacct cttcttttct aatattaaca cctgttttac
    13681 ctattactac tccaagttca acttcatagt ctagttgatt atttatctca aaatgaccat
    13741 ctatctcatc ttctggacct attgcctttg tcacacgttt tgtaaaataa accgtttttt
    13801 gtggttcttc aaaactatca tcaaagtgag tttgagtttc ttcaagatgg tctttatagt
    13861 tcactcccac acataagata tcatgtattg gtttttctat aggaacatgt accgtacatc
    13921 tctctctttt aaaaacatca taattataga atgtactttc atttataata gttttcaact
    13981 taataatatc ttcactagat acattttgaa ttaattcatt taaatcctta aaatttctac
    14041 tgagatttaa agagttaaca tctataataa ataattcatc tttagaaaaa actccagttc
    14101 ttttacattc attttctcct tcagtaaaac ttacaaactt cataatctca ccctttatta
    14161 aaattctatg tatttatttt cttacttaaa gattatacat agttgcatac tctagctatt
    14221 gccatttcag tatatccaag ggactctgct tttgattgtt ttccatttgc tacatctaca
    14281 accatattta acaaatcatc tgttaattct tccattgttt gtggaccata gataactgga
    14341 ctagcatcaa aatccatatt atcttccata tttgcaaaag ttattttatt tccagttatt
    14401 tttattactg gtgctattgg atttcctgat ggtgttcctc ttcctgtact aaataccact
    14461 atttgtgctc ctcctgctac cataccagca acagatgaag catcatttcc aggggtatcc
    14521 attataacaa gacctttctt atcaatttgt ttggcataat catatacttc acttatttgt
    14581 ctatgaccac ctttatgtat acaaccaagt gatttttctt ctaaacttgt taaaccacca
    14641 gctatatttc caggagatgg attaccttct ctaacttctt ctccaaccaa ttttaatgaa
    14701 ttttcatatc tatgtactat atgtaatatt ttttctttta cttcttcatt tactgctctc
    14761 ctagctagta tatgctctgc acctataaat tcagtagttt cacttaaaat tgatgttgct
    14821 cctaaatcga caagtttatc acttaattca cctattaatg gatttgctgc taatccactt
    14881 gttgggtcag acccaccaca ttcagttcct agtataagtt cagatattgg aaattcttct
    14941 tttctaagca aagaagcttc ttgtgccatt tctctagcat aacgaacagc tttttctata
    15001 gtttttaaag ttccatgttc ttcttgtatt acaagagttt ttattggctt atttgttctc
    15061 tcttttattg catcaactac caaatcattt tggcaatttt cgcatcccaa agatacaact
    15121 attacaccat atacatttgg atttgctgca taaccagcca taacatccat agttaattgt
    15181 tggtctgaat gtacttgtga acatccattc tgattattaa aggtaactgc cccttgaact
    15241 tgtgatgcta ttattctagt tgtatctgat gcacacacac tcgtaggcaa tattaatatg
    15301 tggtttctta taccaactct tccatctggt cttttatatc cataaaattt catactgatt
    15361 tcctcctaaa atagtctata tactttctat aatttttata tattataatt tttattacac
    15421 tcactaatca aacagctaaa aacctattta aaaactattt gacactaaaa gcaatgaata
    15481 caacttttat aactctataa atattatttt atatattagt cataacttgt ttatccaaat
    15541 cctctctaac acttctaaca ttatgaatat gtacatgttg gccacttttt atttcctgat
    15601 ttgcttctcc tatatgttca ccatatttag taactttatc acctttggcc atatctttta
    15661 tagccaattt atgataaata gttatatcat ctagtgctgt aatcgttttt actgttttgt
    15721 cttttaattt aaaactaatt tcactacctt tagctattgc ttcaatagct actgcaacat
    15781 tatctttttc atcaattatc actgcattta tcattattct tgctctcctt tataaataat
    15841 ataattttat taatatccta aaatctaatt aaagaattta ttattttacc aacaactcac
    15901 tgtaatataa attaagctgt tattgtacca tcctcatttt gttttgggaa cttaggacat
    15961 ccatacttct tagcccacca actagttata agaggtacta atattgcagt gacaactact
    16021 gcggctgcca cttgtgttgt agctgttgca acatatggtt gccatgatgg gtcaactaat
    16081 gcaacagcag caggtacagc aattgcattt ccagcagttg tagctacagc ccatcctgca
    16141 tatcctggac gttttcctat aaatttatca cagaatacta taaatatacc tcctacaaat
    16201 acagatatta atccaagtaa tactcctgat aaacctcctt ttagtatact tgcaagattt
    16261 atgctactac caagtgcaaa ccctacaaaa ggtacgagta aattacaccc tggctctaaa
    16321 aactctttca tttttttgtc aaaatttcct attatcatac caacaagtat tggcactatt
    16381 gcagccaata atgacaacaa tggtacattt gcaagacctg aagtcccaag agctattaat
    16441 gtaaaaaatg gtccatcatt taaagtaagt aaactcatag ttgcacaatc tgtttcatct
    16501 ccatatgaag tcatcaatga aagatatata ctaccattac tatttgtaac tgcacttata
    16561 acagccaatg ttgtcagacc aaggacacca tccattccaa atagctttcc tataagtata
    16621 cctatcaaag cacctatagc aaatttagaa ccaagaagta taccacctct ctttaaaact
    16681 tttggcattt ctctaaattg aagctgagtt cccaagcata ccagttgaac cccaattata
    16741 gttgcagacc caacacttga aaatactgct gtcgtaaacg aacctatttg tacaatatct
    16801 gggcaaaagg tattaatgaa agctgctata aacattggca ctatcatcat acctgcagga
    16861 atttttttaa taaaatccat tatcataaag ttaatcctcc taaaacatga tatttaatta
    16921 ctcttattaa gtttttagct ttaattctaa cggccgtttc ggttcgtatt taagaactaa
    16981 gtttttaatt acgaatcttt gaattaatga tagcattttc ctatttatga gtcaataaaa
    17041 aaaataaatt tttgtcaaaa ttttttcaaa ctttctgata ataccttgta tattatcaaa
    17101 tgacttcctt atatgcaaaa atacagcgta tttaaaatta tatagtattc aa
//
LOCUS       DAEOSP010000066        17012 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.066, whole genome shotgun sequence.
ACCESSION   DAEOSP010000066 DAEOSP010000000
VERSION     DAEOSP010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17012
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.066"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(124..648)
                     /locus_tag="KRH54_001807"
     CDS             complement(124..648)
                     /locus_tag="KRH54_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG8579470.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(713..1465)
                     /locus_tag="KRH54_001808"
     CDS             complement(713..1465)
                     /locus_tag="KRH54_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG8579471.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1496..2794)
                     /locus_tag="KRH54_001809"
     CDS             complement(1496..2794)
                     /locus_tag="KRH54_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBG8579472.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2811..5075)
                     /locus_tag="KRH54_001810"
     CDS             complement(2811..5075)
                     /locus_tag="KRH54_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBG8579473.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5330..6148)
                     /locus_tag="KRH54_001811"
     CDS             complement(5330..6148)
                     /locus_tag="KRH54_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG8579474.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6141..6839)
                     /gene="cbiQ"
                     /locus_tag="KRH54_001812"
     CDS             complement(6141..6839)
                     /gene="cbiQ"
                     /locus_tag="KRH54_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBG8579475.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6842..7120)
                     /locus_tag="KRH54_001813"
     CDS             complement(6842..7120)
                     /locus_tag="KRH54_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG8579476.1"
                     /translation="MSAKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEITK
                     INPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7110..7862)
                     /locus_tag="KRH54_001814"
     CDS             complement(7110..7862)
                     /locus_tag="KRH54_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBG8579477.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8044..8236)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8368..10140)
                     /locus_tag="KRH54_001815"
     CDS             complement(8368..10140)
                     /locus_tag="KRH54_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBG8579478.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10154..11083)
                     /locus_tag="KRH54_001816"
     CDS             complement(10154..11083)
                     /locus_tag="KRH54_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG8579479.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11311..11499
                     /locus_tag="KRH54_001817"
     CDS             11311..11499
                     /locus_tag="KRH54_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8579480.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            11492..12193
                     /locus_tag="KRH54_001818"
     CDS             11492..12193
                     /locus_tag="KRH54_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8579481.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAIIAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGQVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12195..13118
                     /locus_tag="KRH54_001819"
     CDS             12195..13118
                     /locus_tag="KRH54_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8579482.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13275..14198
                     /locus_tag="KRH54_001820"
     CDS             13275..14198
                     /locus_tag="KRH54_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8579483.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKEREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14191..14928
                     /locus_tag="KRH54_001821"
     CDS             14191..14928
                     /locus_tag="KRH54_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8579484.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            14939..15712
                     /locus_tag="KRH54_001822"
     CDS             14939..15712
                     /locus_tag="KRH54_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8579485.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(16239..16847)
                     /locus_tag="KRH54_001823"
     CDS             complement(16239..16847)
                     /locus_tag="KRH54_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBG8579486.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5480 a         2947 c         2142 g         6443 t
ORIGIN      
        1 aaataaatta aaaagtgaaa tgtatttgtc taaaaacata ataacatttc actttatcct
       61 catttttaag taatatatat ttttaatttt tatttataac taaatctagt ttttatataa
      121 attttagttt ttttctacat ctgagtaatc tattccaaaa gtttctacag taacagattt
      181 cataacttgt ggagtttgtg gcttatcagc tgaatctgtt ctaactgatg ctatttcatc
      241 aactgtatcc ataccttcta tcactctacc aaaacctgca tactgaccat ctaagtgtgg
      301 tgagttttta tgcattatga aaaattgaga accagctgaa ttaggtgcca tagttctagc
      361 cattgatatg actcctcttt catgttttaa attgttagta aatccattac cactaaattc
      421 accttttatt gagtgacctg gaccacccat tcctgtacca tttgggcatc ctccttgtat
      481 cataaatcct ggtattactc tatgaaatat tattccattg taatatcctt catttactaa
      541 tgatacaaag tttttaactg tatttggagc tatatttgga tataactcaa ttttgatttc
      601 ttttccattt tccatttcta tagttactat aggattttta ttttccataa tttattacct
      661 tccttttcat tagttaaatt acataattac atttattata ttatactcta aattaaagtc
      721 tatcaagtat attttttcct atttcatatc cttcttgata aatagtttta caaaactcat
      781 catcaaagtt aagcgtgtct ttaggagcaa attgagtatt aggtctaact actattattt
      841 tatcttcatc atatactttt ttaatatctt ctggcaactc ataattatat tttgtggata
      901 ttaaaataat tttatccaca ttatccccaa ttaatggttc aattaatgca ctcctaatta
      961 aacctccatc taatttgaaa ccatcataaa ttccttcttg cacatctttc acaaattgtt
     1021 ctttaaatcc aagagttgcg tttggatttg atggtaacaa cccactgtat gaaatgctga
     1081 taatggcttc attacgaggc atttttgata cgtcaacaat cttttctgat actactttat
     1141 tatcaacttc aacataatta acataaaagt tcatttcttc tatattacta tcatctagtt
     1201 cccttaggtt gtctatagca ggagaattat ccacagtatt atttactatt tttacatttc
     1261 cattctggat atcttttata tttgtccaca ttttctccaa gttatcaaca ttccctgtgt
     1321 acaaataata tccatttatt gctcctattg atgtccctga aatcgaataa aatttattta
     1381 ttcctccatc atacaaggct ttaactactc ctgcttgata agcccctttt gctcctccac
     1441 cttctaaaca taatcctaat ttcatagtct catctccaaa ttgtttatct tatatttata
     1501 tttcaaaaaa cttactaaat gttttatata tactatagtg gtcctcaaca tttcctaatt
     1561 ctcttataga atgcattgct aaaattggac ttcccacatc cacagatggt atatctatat
     1621 gagtagaaga tattggacct atagtacttc ctcctctttc atctgaccta ttaacaaatt
     1681 cttgatactc aacattagct tctctacata tatttttata aatagctata gaatatccat
     1741 cactagtata tgcctgacta gcatttatct ttataaccgg accttttccc attattggtt
     1801 tattagttgg gtcatgcttt tctggaatgt ttggatgaat agcatgagct aaatctgatg
     1861 atatcataaa agatgaataa atagactcaa agaatccctc tctatcctta cccattgata
     1921 tgcaaattct ctccaatata tttaacaaca tgtttgaatc tgcaccttgt ttagtagagc
     1981 taccaacttc ttcattgtca aatacagcca ttacatttac accatgttga cctttagtat
     2041 taacaagagc attcaaacta gcatgtacca ttgcaagatt atcttgtctc ccaatagata
     2101 taaattcatc attagcccct attaaacttc ctttttcaaa ttcatataaa aataaatcaa
     2161 aatctataat atcttccata tttacatcta agttagatgc tatttgcttt ataagaaaat
     2221 tatctttttc taatgtatca tttaacatcc caactaaagg taacatatct ttttgcttat
     2281 tatatgaatg accatcattt atacttctat ttagatgtat tgctagattt ggtattatac
     2341 aaattggctt tttaaagtct ataagccttt catttggttt taatatacta tcacctttta
     2401 aaactactct accagctatc ccaagaggtc tatccaacca tgtacttaat attgctcctc
     2461 catagcattc tgtatttaac ttcaaataag atttttcagc ttctatttct gcatttggtt
     2521 taatactaaa tgtaggagaa tctgaatgag agcctattat tctaaatcca tctctttcta
     2581 taccttctga atttactaca aaagctacta tagctgataa attcttagta acataatact
     2641 ttccaccaac tcttagattc catctttgat tcaaaactaa ttcttcaaat ccatttttat
     2701 ctaagatttc cttagtactc tcgactgcat gaaaagacga cggactatca tatataaagt
     2761 ctattaagtt tttagcaaag tttatactat ccattcctca tttacctcca ttataaaata
     2821 tttagtgttg tatattcttt atttattttt ttaattccct gttggtcaaa agctatagta
     2881 acatctgtac caattataga aaccactgtc ccaaccccaa atttaggatg atgtacctta
     2941 gaaccaagtt ttatatcatc tggattagtt tctttattag aatcttttat tgtagcatta
     3001 actttatctg caactttaga tttgcttata gtactcatat actttttagt atatttatct
     3061 aacatattat aacttgcttt agaatagctt agttcttttt ttactttgta caatctttct
     3121 atacattctt ctggcagttc ttccataaat cttgaagcta tcgcaacatt tgttttacca
     3181 tataatgttc tcttctctgt caatgacata tatagctctt ctttagctct agttatacca
     3241 acataacaaa gtcttctctc ttcttctatt tgtgaatcac tcatagattt tatagctctt
     3301 gatataggaa ataacccttc ttccatacct gttaaaaaca caacagggaa ctctaaacct
     3361 ttggatgtat gaattgtcat aagcgaaacc ctatcattgt cttcttcatc atttgattca
     3421 gatgtaagtg ctatacttgt taggaatgtc tctaagtctt tattttcata agtatcatca
     3481 ttactacttt caaactccaa agctatagaa ataaattctc tcaaattatc aattctgtct
     3541 tgagcttctt cacctttgcc tgttaaatct ttttcatttt tatttcttat ctctacaagc
     3601 tcgtccatat atcctgtagt atccaagact ttttctataa gtttgctaac aggataaact
     3661 tcttttatag ttctaagagt ccctataata tcaacaaatc cacttatact tgcccttgcc
     3721 ttagtagata tatctgaatt tgtttctata tcaattaata ctgagtatat actttcctgt
     3781 tttaagttag ctctatcttc aattttttct atagttctaa gccctatacc tcttctagga
     3841 acatttataa tcctttttat agatatatca tcttgtggat tttgaataac tcttaaatac
     3901 gccaacaaat cctttatttc ttttctctca tagaattttg taccaccata tatattgtat
     3961 ggaatttgac ttctatttaa agcgtcctct acaggtcttg cctgtgcatt tgctctataa
     4021 agtactgcaa aatccttata aggtctattt tcttttctag ctatttgtgc aattgaatca
     4081 gcaatgaaat cagcctcttc tatttcgcta ccagttaatt gtatctttat aagttctcct
     4141 tctttttttt cactccaaag cttttttctt tttctttcta tattattaga tataacttta
     4201 ttagctgcat ctaatatcac ttgtgtagac ctataatttt gttctaactt tacaacatgt
     4261 acattgtcat aatctttctc aaattcaagt atgtttctta tatctgcacc tctccatcca
     4321 taaatacttt ggtcatcatc tccaacaaca caaatatttt gatgttctct tgctaataat
     4381 tttatcaatt catattgagc tttacttgta tcttgatact catctaccat tatgtatcta
     4441 aatctacttc tgtaatatgc taatacttca tcattagcct tgaaaagttc tacagtttta
     4501 agtattaaat catcaaagtc aagtgcacta tttcttttta aacggtcttg atatagcgca
     4561 taaatatctg caatcttaac cattctatta tctgccatat tgatatcttt aaactgcttt
     4621 ggagtataaa gtttgtcttt tgctcctgaa attgcactta taactgcttt tggttcaaac
     4681 actttatcac ttaaatttaa ttcttttaga cagtctttaa ccaatgtaac ttggtctgct
     4741 gaatcataaa ttacaaaact tctattgtaa ccaatcttat ttatatcttt tcttagtata
     4801 cgaacacagc aagaatggaa tgtacttatc cacatatcct tagtttcagg acctatattt
     4861 tgttcaactc tctctctcat ttcatttgca gctttgtttg taaatgttat agctaatata
     4921 tttggtgctt gtacaccttt atcttctata agatatgcta ttcttgttgt taatacctta
     4981 gttttacctg aaccagcacc agccagtata agtactggac cttcagtctt ttctactgct
     5041 tctctttgag caggatttaa tgtatctaaa ttcatatttc tcctcctgtt aacagccata
     5101 aatatatttt aatctaaaat atgaattact tcaaattata attatgtatt atattaatag
     5161 attatttcca atatattaca attaaataac atttttccat ataatttatt tcattgttta
     5221 ataaaaaaaa taaccttatg atttggggaa tcataaggca cctttaaggg gtctaggtta
     5281 agggttatta tatataaatc tattttttta aaataggttt agggcttaat tatttatttg
     5341 tattaagttc tctttccttc caatacttat acaaatcttc ttctttctta aataagggta
     5401 aattcatatt tctatgtact tttactaacc atggggattc taggccagct tgtataatat
     5461 tattttcatt tataaacaca ttttctgctt ttccttcatc aatcaaaatc cccttatcca
     5521 atacatatat gtaatcacat atttcataca ttaaattcat atcatgactg gatattacta
     5581 tttttatatt atttttattt aatcccttta ttatatctac aatagaccta gtacttactg
     5641 ggtctaaacc agctgtaggt tcatcaagta atacaatttc attttccatt gctataactg
     5701 atgctattgc cactcttttt ttctgtccat agctaagaaa atgtactggc ctgtctataa
     5761 aatctattcc attaacagct actagagctt tgtttattct ctctttaatt gttttttcat
     5821 ccataccaat gtttctcatt gcaaaggcta tatcatcata tacttttgaa taaaaaattt
     5881 gtttttctgg gtcttggaag acaataccta cattttttct aaggttgtat aaacttctct
     5941 tatcatattt taacttttct tctttaaaaa gaatttctcc agatgtaggc tttaatatac
     6001 ccattaaatt cataaataat gttgattttc ctgaaccatt tgaacctatt atacctataa
     6061 catttccctt tgaaaaatcc atattaatgt ttaatagtgc attggtattt ttttcatatt
     6121 gatacgttaa attatttatt ttaaacatca ttatccccta ctatgtgaaa tttaccatca
     6181 tacaatttta tgtctaatga tatacacata tcatcatacc ttgtcataag tcttttgtac
     6241 agcatacttc caagaatacc taaggattta tatgaagttt ttaaatttat atatccaaat
     6301 cttagctctt gtgacttgta tatttctgaa aattcttcta aaaatatgaa tataaaccta
     6361 tatattaaca ttgataactc gacaaaagtg tcaggtaaat gaagtttttt aaaaaagaat
     6421 attaattgat taaaaggtgt tgtaagtata caaaaataca cacaagtcaa acaagacatc
     6481 gctctaaata acacatgaat agacatgtca atagacgcct ttgatattcc tacattaaaa
     6541 ctaaaaaatt caaaactata taataaatct gctttattaa aagatatatt tacaagagtt
     6601 attcctatac taagaaataa aaagtacatt ggtattctaa gtaattttaa ataatttttt
     6661 aaatctattc cagccacaca aacaaccaaa atactcatta aaatcataat tccacttaaa
     6721 ataaatatat tttgtattac catagaagca atcagaaaaa ttactcctat ggctactttt
     6781 ttgtttggat ttactttact caatctattt gtatatgcat atttatcaat tataagcatg
     6841 attaacttct ctttcctttt tgaagaccta atatgtatcc tattacacca gcccctaaag
     6901 ctgcttgtgc agagaataat aaactttcta tttctccact tggtggctca tatggactgc
     6961 taaaccatgg tttgtaattt ggatttatct tagtaatttc actttcagct tgaccatctg
     7021 ctcctccata ttcagcacct gagttgacaa gtaatgggaa tataataaga gctactacta
     7081 ataaaagtaa taatatgtta gtttttgttt tagcactcat tagctttaac tcctttatca
     7141 ttattttcta gtatgctttt ttctccgttt tctgtaatta aattatatac aattacagta
     7201 agtatacctt ctgctattgc tattggtatt tgagtcataa agaaaattcc taaaaattta
     7261 attgctgaag ccattacccc acctgatggg tctgggaaag ccagtgctaa ttgtattgaa
     7321 gttattgtat atgtggctaa gtctcctatt gctgctgcaa gaaatactgg aatagcatta
     7381 tttccatctt ttttctttaa agccttaaat attaggaatg atacgattgg ccctattatc
     7441 gccattgaaa aagcattagc tcctaaagta gttattcctc catgtgctaa taacaatgct
     7501 tggaatatta aaactatagt tccaagaaca aacattacgc ttggtccaaa cattattgct
     7561 ccaagaccta caccagtagg atgtgaacaa cttcctgtta ctgaaggtat ttttagtgct
     7621 gataatacaa acacaaatgc tccacataaa gctaataata ctttcttttt agggtcttgc
     7681 tttactattt tacctattga ttttaatccg aataagaaaa atggtataaa tataactccc
     7741 catgctatag accattttac tggtaagtaa ccttccatta tatgcattgc atttgatgta
     7801 actggagtta aaatgattaa cataagtgca gctataaccc ctaatttaat attctgtttc
     7861 atcttagtat ccctctctcc ctttctaata attaaaacat aataatacat gtctgattta
     7921 tttaataaaa taaaaaagcc taactcgatg gttaggcttc taattttcca caatctaaat
     7981 acaaacacat aaaacaaaca tatctgtata attattataa gaaactttct ccatccccga
     8041 agaaattact ttaatatttt aggcaggtct cctgacttag cttcatccta ctcctcttcc
     8101 ttcccatgag atataaaaaa tatcatcata gtggctaaca aaagtttcgt ctgcatcaca
     8161 gtagcggggg ctgtaaagga atttaacctt tttccctatt aatcttgctt aaagaaccta
     8221 taatattata tatttaattt ttaaattctt cttaaattat tttctatata aatcattgta
     8281 taccttttct gtgaaaattt ctacattttt tataaatttt attcattttt atatttttat
     8341 gtatataaat tcttttttgt aagtttatta atttctaatt atcttcttta aatacattga
     8401 aactacatca gaaaatctag ttatatctct tgaatatata aaatcatttc catatattaa
     8461 tctttcagct tcaagttctg attcttttcc tgtaaatact cctaatacta atatattttg
     8521 ctttctagct tttctcactt cattggctgt atctctcaaa ccaactatac ccctataagc
     8581 cttttctcct cttaatgttc tctcactact ttttcctatc tttacatcat ttggtcttcc
     8641 atcgctgaga actatcagga ttttattttc ttcatctcta tttattagac cactacatat
     8701 agattttatt gccaaaccat ctctattatt accagcacaa aaatattcaa atatattttc
     8761 actatttttt atactatcat cataatcttt aaatcttttt aatattgtat agtctaaaaa
     8821 actagaaaat cccatcactc tatttggtat gccaacttct gttagagcat ttgcaattat
     8881 gtaagcttga attgccacat ttccctgatt tctactttga gagccacttg catccaataa
     8941 tatatcaata acgtatttcc ctttttcatt ttctatatct ttataaaata ttttattatt
     9001 attacttcta ccaactctcc aagctctatt tgctaatata gtaccacaat cagaatatac
     9061 tctactttta gcattttctt ctattaaaat tcttgataga ctttctttta atctctttat
     9121 ttgctgtcta tacattctag catgttctct aaattttcct aaattatttt ctttttgtct
     9181 tgttacatat tttagttgaa atacattatt acactcactt ctaagaactc catctgtaaa
     9241 gtgtattcta caaccttcat gaacatttct acaattttgt gtctcaattt ttttaatttc
     9301 acctttactc aaataagttt ttccataata atgttctatt tttgaatata tcttatttgc
     9361 agtgtcttca tcaacatata taactcggtc tgctgaattg ctacatttag aatcatcaaa
     9421 atcccctaca ccttctacaa gcatggatga agatattgaa tctatatcac tttcaataat
     9481 attttcttct tcatcactat aaagttcttc atacatgaaa tcagcaaatg tatcaaaatt
     9541 tacttctaca ctttctacat tattttcatc aataagacta ttttcttctc catttaatat
     9601 gtattcaaaa taagttaaat atataacatc taatgctttt aaaatctcca taatatcttg
     9661 attggtatct atatttctta tatcattaac tattcttcta gtcatactat ctaatgctgg
     9721 gtgcttgtcc atactataaa gtacaaatgt gtatttaatt ttatccataa tattatcatt
     9781 gtgtattttg gtaaaacgtg aaacaatatc tttataagca tttcttctta tgtcttctat
     9841 ccctggccta tctttaatga ccttttcttc aactattgta tttaatataa cttgaattag
     9901 acccagtata atatctttgt tgtatccttt tcttattcta tttgtaagat attttttaac
     9961 tatatcccaa tctacatact ttcttctagc acctgcaatt attgcaaaat atagacctat
    10021 ttcttttgaa gagtaatctt tttctgatat atctatactt tcattgtaat caccagctat
    10081 tgtccatgat aagttattag ctctattttc aaattcataa tcatcatata tccatttcat
    10141 aatctcacct atattaaata tgacttattg gaaacacatc tatactctcc catttattag
    10201 gtattctagt tttaattaca tctccaacca tttctttctc atatacatca aaagtctttc
    10261 ctgtaagtcc catattaatt gcaagagttg gttttaatcc tcttcttata gtcttaagag
    10321 atgccataag accacgtaaa tcaatagctt tactagatat ctctccattt tgagacttca
    10381 attgtaaatc taaaaatatt cctgcaaagt gtattagttt ttcttcatca gcatctgaaa
    10441 attcattttt taaaatcatc attaacttat cttcttctat aggaggaata tctattacca
    10501 taaatctaga taccagtgct tcattaagtt cttttgttcc tgcatattca taattcatag
    10561 tcccaataaa tcttgttgct ggatgtagat tgactctctc atacccaggt acatcaatta
    10621 ttcttctata gtctaaagct gaatgtacca caactatagc atcatttttt gccatattaa
    10681 tttcatcaaa caccccaaaa cctccattaa tagcacactc ataaactgag cctcttctaa
    10741 gtttaacttc attgtctata aatgtatctg tacctataag tgttgaactg tctgtattta
    10801 tatgaaatga tgtattccac tgaggtctat taaatatttc ccctagatta tcagcaagta
    10861 agttttttcc cgttgccttt ggtcccgata ataatatatt ttcttcttct aaaatagctg
    10921 ctatacacat agacaagata tctttaccat aaaaataagt tttagacttt gttactctgt
    10981 attctaaatc tttttctaat ccataataat ttctaaaata cagtacatct tcaataagtt
    11041 tttcatctac cccttgcacc cttagattat ctattatatc catattttta ccccttttat
    11101 ataaatagta tttgtttcat ttcttatcac cattttagca taaaaggata taagctaaaa
    11161 ttgatttttt aatctcccca ttttaagtaa aaccatgcac cattttatca ctacaaaatt
    11221 aagagtacta cttactgata tttcttgatt ttgttcgtct atacgtatat aatatatgct
    11281 gataaattat gaggaaagga tgattgaaat atggaatata tgtcttgccc agaagtagca
    11341 aaaaaatggg gtatttctga aagaagagta caaaaactat gtgaagaaag tcgcatacca
    11401 ggagtttcaa aactcgggta tatgtggctg ataccaaaga acgcagaaaa gcctgttgat
    11461 ggtagaacaa aaagaagaaa ggaagtacaa tatgaatagc gttttgatta tagatgatga
    11521 caaagaactt tgtgctctta tgaaaaaatg tgttgagcaa gaaaatttat ctgctattat
    11581 tgcacattgt ggtgcagaag gtctacattt ggtcgataaa aatagacaca cactctctct
    11641 tgtaattttg gacataatga tgccagatat agacggtttt caagttctga aaaaaattag
    11701 agaaacaagt aatattccag taattatgct tactgctaaa aatgacgagg atgataaagt
    11761 ttccggacta cgtttaggtg ctgacgatta cctaacaaaa ccgttcagca taaacgaact
    11821 tatggctcgt gtcaattctc ttattcgtcg gtatactatg ttaaaccctg cttttataac
    11881 agacacagat tgcataataa taaatggcat gattattgac aagacaaacc gaattgtttc
    11941 tgtcaataat attcctgttc aacttacaag caaagaattt gatttacttg cttttctggc
    12001 ttctaataag ggacaggtgt ttaccaaaaa acagatttat acacaagtat gggaagaaga
    12061 atatgctttt gacgatagta acatcatgtc tttcatcagt aaattgagaa aaaagattga
    12121 accagacccc aaccatccat tttatatttt gacagttcgt ggtgtcggtt atcgttttaa
    12181 taaggaggct taacatgaat acaagtgtct atctgctttt gatattggtt cttagcctga
    12241 ttattatagc gtaccttgtt tctgtcctgc tacgtgtccg aatacaactt acacttataa
    12301 aagatgcttt agaggatata aaaaatggaa accttaatcg acgtatttta acagatgaaa
    12361 acgacattac aaaacaaatt tgctatgata ttaacgaaat agcaatcaat agccaatttc
    12421 aacttattca gcaaaaacaa tcagaacaag cctacaaaca gctaatgaca agtctctccc
    12481 atgatgtaaa aactccactt gcttctttgg tcggttattt ggaagcagta gaaagtaaaa
    12541 ttgttgtagg agaggaaaaa gacgagtata tccatgttgc gtctaataag gctcattact
    12601 taaagaattt tgtagaaaat ctatttgaat gggtaaaatt ggattctaag gaacaagttt
    12661 ttcattttga tatttttgac ttgaatgaat tatcccgtaa tattatatct gattggatac
    12721 ctgttttaga aagcagtcat tttgaatatg aatttgatat accagaaata gagtacttct
    12781 tacgtataga cgcaaatgca tatacccgta tcattaataa cctattacaa aatattatta
    12841 cccatagtag tggcaacaaa atgacattga gaatttttga aaacaaagag caagctcaaa
    12901 ttataataac agataatggg aaaggtatat cttcagacaa tctcccacat atttttgaaa
    12961 ggttgtacca atgcgaccat tcacgagctt caaagggaaa tggtttaggc ttggcgattg
    13021 caaaggaact tattaacgct cataagggaa atattaccgc caacagcact cccggtatgg
    13081 gaacagaatt tacaatattg cttcctaaag ccctgtaaaa gtacgaggct ttcttactct
    13141 aaaacaagat aaaagcaaga ttatggcaag gttttggcaa ggttcatgtt ttataattct
    13201 aattgtagtt gattttgacg ttgtatcaac tatcttttgt agaaacaaat ttattaaaag
    13261 tgaggaaatt atatatgaat gattttgtga ttgaaacaaa gcaacttaca aaaatttatg
    13321 ggaatcaaac agtagttgac aaagtcaatc tccatgtaaa aaaaggccga atatatggtc
    13381 tgttaggacg caacggagct gggaaaacca caattatgaa aatgatttta ggattgactt
    13441 caatcacgta tggcgaggta gatgtatttg gagagaatat taaggaacgt gaaaaacaag
    13501 tatatccccg tatcggtgca attatcgaaa cgccaggatt ttatcctaat ctgacaggaa
    13561 cggagaactt ggagattttt gcaaaactgc gtggtacagc cgcccctaat tctgtaaaaa
    13621 gagcattgga aattgtaggc ttaccttata aaaataaaaa gctgtttagt aaatattcac
    13681 ttggcatgaa acagcgactt ggtattgcca acgctatcct gcatgaccca gaactgttag
    13741 ttttagatga gccaaccaat ggtcttgacc ctattggtat tgcggagatg agaaagttta
    13801 ttagggactt gagcgttgag catggaaaga caattcttat ttccagtcat attctttcag
    13861 aaatctcatt gttggctgat gatattggaa ttatcgacca cggtgttctt ttggaagaaa
    13921 gtagtatgag tgaactagaa aagaaaaaca gtaaatatat cctgctacaa gtttcagata
    13981 ttccgaagac atcacttatt ttagagcgtc aatttaacac gaaggattat tccgttcaag
    14041 atgactacac gttacacctt tataacacta atctggatat gggtgaaatc aataaagcac
    14101 ttgtaatgca gaatgtaact gttattagtt ctcaactttg taatgatact ttagaggact
    14161 actttaaaaa aatcacagga ggagagggca ttgcttaaac ttattcaaac agaatttctt
    14221 aaactgcgtc gtaggaaact tatttggctg atgttgttgt ctgctctcat tatgccgttt
    14281 ttcgcactac tatacttcaa ttattttggt aaaacaggtg ttgagccaat aaagttttat
    14341 aaatggtctg catttggtta tacactttgg attattttgc ctgttgtttt gggtatattt
    14401 tgcaccatgc ttatacacga agaaaaccag tatgatatgc tcaaacagct ttggattgta
    14461 cctattagta aaatgggata cttttttagt aaatttttcg ttgttttgat ttactctatt
    14521 tgttttatgc tgattacagc cgttgcttct gtgttattta gtgtactttc tggatatgtc
    14581 gtatttagct gggcaagcat tttatatttg ttgaaaaaat gtttagaaat tggtgtaatc
    14641 actgcttttg taatgttgcc gattttggca atcgctacat ctcaaaaagg atacattctc
    14701 ccaatttgta tcacattggt atatgccttt tccggttttg ttataatgac aataaatatg
    14761 tatttacacc cattatccag tatgggagtt attatcatga gaaataaaga cattccaggt
    14821 cttatatttt cacaggaaat taatgtccca ttagcatttc tttgtatttg tatttggagt
    14881 attgtatcag tattgtttgc aaatattgca ctaggaagaa gaaagtgagg tgtagataat
    14941 gtggaaatta ctttgggctg aatgtcaaaa aattcgtcgt tcaaaaattg tttggattac
    15001 agtttttgca tccattatga tagcagtcat agtttttgca ggggggcagg atgtatataa
    15061 tgacccagat ttacattatg gcttaaagag cgtttatgac ggttcacggt atatagacaa
    15121 tgcaggttgg tatatggacg cagtacaacc ttggtcaact ttttttgttc ttcctgctgt
    15181 cattgcccta ttaggaagct atattatttg tcgtgaagaa gaggaggaca ctattaaatc
    15241 cctgcgtctt attcccataa atgaaacaaa tttaaccata gcaaaaatga ttattacttt
    15301 tatattcagt atacttcttt atttgttact ctttactata acatttttga ctgaatccgt
    15361 tttacatttt tccgatttat cgacaaaatt ggttttaagt tgcatgaaag agtattttct
    15421 tgatggcata ggtgtattct ttgcaatttc tcccattatt gccttggtat cgcgcatgaa
    15481 aaaaggatat tggcttgcat tagtattcac agaagtttat tctattgctg gactatttgc
    15541 gggtatgtca aatgtcttac aaaccttttt tcctattgcg gcaatattta atatttctgg
    15601 atactatata actactggag aaaaaattat ggggagtatc ataagtttat tactttgtgc
    15661 ctgcctttct gcatttatac ttaaagggct aaaacacaat gaaaaaaatt gaaataggta
    15721 tatcaaaatg aaaaagataa aaaaactgat tattctaatt gtattcttgt ttctttttgg
    15781 catgttacac tgttatactg aatggaaatt caacaataac ccacctaaag gggtaataga
    15841 aagaataaat gagtggactg gattagtaac ttatgttaat tatagtgaat gtaatattag
    15901 caagttattg tttcgtattt taatactata actttaaata tttggtttta atcttccaaa
    15961 cgaatataaa aaaactgtca cagaggcaac atatttttga atatttataa agtggtagtt
    16021 ttgattttca aagctaccgc cttttcttct atataagcaa acttatcctc aacaaagtgt
    16081 aaaacatggt acattttaag aaaaaagtac cacagacaca agcaataaaa gaaacgccga
    16141 gaacctacaa aaacgtagtg cttatgcaga tttacgacgc cttctatttt ctcaaccgtc
    16201 ttaatgacct tgtacaaaag aaatttttct aatatttttt ataatcttat cttgtgatgt
    16261 tttttatgta taaatgactt aataacaaat ccaataaaaa ttatggatat tggtataatt
    16321 agaacgagga atttataatc attataatgt gacattattc ttacccaact ctcagaaaaa
    16381 atatatccta tactgattaa tgaagtattc catatggtta ttcccacgat agatgatatt
    16441 acaaaagtta taatattttg ccctgctata cctgctataa aagatatgta tgttctacat
    16501 aaagggatta atctacatac acaaacagaa atagaagagt atttattaaa ataatcttga
    16561 gatgcaaata ttcctttctt tgcctttgga catatctcta ttattttatt tatgatagat
    16621 ttacctcccc aatttcccac tgtataacaa aatatcgaac caagtatacc tgcaattata
    16681 cttaaaatta aaattgtcag aaatccaaaa tgatttcttg cagcaattgc tccacataaa
    16741 ggtaaaacaa cttcgctagg taatggaaaa caagcatact caatcataat taatacaaat
    16801 acagagatta atccatattg taaaataaaa ctattgatta cagacactta cttatctatc
    16861 tcctttctta tttaaaggtt ttaagggtac atgttctaag gattctcacc ctatatttaa
    16921 agaatcaatc tctttaaact attatatgta tttacacaaa gcatttataa catactttaa
    16981 aaatagtacc ttaaaataag aaacttaata aa
//
LOCUS       DAEOSP010000067        16805 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.067, whole genome shotgun sequence.
ACCESSION   DAEOSP010000067 DAEOSP010000000
VERSION     DAEOSP010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.067"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            222..2081
                     /locus_tag="KRH54_001824"
     CDS             222..2081
                     /locus_tag="KRH54_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG8579487.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2373..2972)
                     /locus_tag="KRH54_001825"
     CDS             complement(2373..2972)
                     /locus_tag="KRH54_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG8579488.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(3075..3872)
                     /locus_tag="KRH54_001826"
     CDS             complement(3075..3872)
                     /locus_tag="KRH54_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG8579489.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3985..4091)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4183..5169)
                     /locus_tag="KRH54_001827"
     CDS             complement(4183..5169)
                     /locus_tag="KRH54_001827"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBG8579490.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5653..7383
                     /locus_tag="KRH54_001828"
     CDS             5653..7383
                     /locus_tag="KRH54_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBG8579491.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7493..7573
                     /locus_tag="KRH54_001829"
     tRNA            7493..7573
                     /locus_tag="KRH54_001829"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7527..7529,aa:Leu,seq:tag)
     gene            7908..8423
                     /locus_tag="KRH54_001830"
     CDS             7908..8423
                     /locus_tag="KRH54_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HBG8579492.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      8915..9019
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9229..11703
                     /gene="gcvPA"
                     /locus_tag="KRH54_001831"
     CDS             9229..11703
                     /gene="gcvPA"
                     /locus_tag="KRH54_001831"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBG8579493.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISTLKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11703..13160
                     /gene="gcvPB"
                     /locus_tag="KRH54_001832"
     CDS             11703..13160
                     /gene="gcvPB"
                     /locus_tag="KRH54_001832"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBG8579494.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDPDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINVLKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            13583..15940
                     /locus_tag="KRH54_001833"
     CDS             13583..15940
                     /locus_tag="KRH54_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBG8579495.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDDNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(16026..16343)
                     /locus_tag="KRH54_001834"
     CDS             complement(16026..16343)
                     /locus_tag="KRH54_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBG8579496.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         6361 a         1940 c         2737 g         5767 t
ORIGIN      
        1 aagtttataa attatattaa tagtaataat ctataagaac ttatatatta gaaaaaaatt
       61 tttgtatata taaaggactc taaagatgaa ctatgtaaaa caaggtttat atattctaat
      121 tgtatagaaa aatttattgt aaacgcatgt atattattag aaaaaatagt ttggaggtat
      181 tcaataaaca taaaaaacaa tataggggat aaaggtgttt tatgaagaga tttttgagga
      241 gaattatctt agttttattt attttgttga tttttataag ttttatttct attaaattaa
      301 ttcataatgt tggagattat ggaaaattga ttaattatgt tgggattgtt cgtggagcta
      361 gccaacgtct aactaaacta gagatgaatc atcaaccaaa tgatgaatta atagaatata
      421 ttgatgaaat cctacaggag ttgattacag ggcatggaga ttatggttta gtaatcactg
      481 attgtaatga atacaatgag gacttacttt tattagaaaa aaagtgggaa gatttaaata
      541 ttgaaattaa aaaagtaaga atgaaagaac agaataacca gttattaagt ataagtgaag
      601 agtttttttc tcttgcaaat gatacagtgt ttaaaataga aaacttttct aaagaaaaaa
      661 gtaattactt aatgacttta ataataatta tatctattat aggtatatta gcatgtatca
      721 tattgatttt acaatatagt aaaaaaatgg ttagactgga aaaattaaat gtagatttaa
      781 aaaatattgc atataaagat gaactaacag gagtaaatac tatagaaaaa tttaaattgg
      841 atgcaaatca aaatatttgt atacataagg ataagaaatt tgctgtattc tatattgatt
      901 ttgaaaactt taaatacatt aatgatatat ttggatatga ttatggtgac atgatactga
      961 aaagatatgc taatcttatg atgaatgaca tagggaaata tgaaatattt gcaagagaaa
     1021 ttgcagatag atttgttgcg ttaagatgct atgtagataa ggaagattta gtggtaagac
     1081 aacgagatgt agacaatgaa ctaattaata taacaaatga aattaaaaat aaacatagta
     1141 ttactgttgt tagtggaatt tgttgtattg aagatgtaaa tgaaaaatta agtatagatg
     1201 gtttaataaa tagagcaaat tttgcacaaa aaactgttaa gaataaacca ggtactaatt
     1261 atgcttttta taatgacagt attcgtaaaa aaatgattga agaaaatact atcaaaagta
     1321 gaatacatga agctattgaa aaaagagaat ttatagttta tttacagcct aaagttaatt
     1381 tacatagtca aaaaataaat tgtgcagaag ctttagttcg ttggcttact ccggataaag
     1441 gtattatatc tccagcaata tttattccag tacttgagaa gaattttttt attgccttag
     1501 tagataagta tgtttttgag gaggtttgta aatggattag aaagaggtta gatgaaaata
     1561 aaccttttgt tcagatatca gttaatgttt caagaataca attttataac actaaatttg
     1621 tagaaactta ttccaatata caaaataaat acaggatacc taaaaatact atagaaattg
     1681 aatttacaga gagtgttgct tttgagaatc aaaaacatct tttggaaatt atacatgatt
     1741 tgcatgaaaa tggatttact tgttctttag atgattttgg aaaaggttat tcgtctttaa
     1801 gtgttttaaa agatttacca tttgatgttt taaagcttga ttctatgttt ttcaaggcta
     1861 gtttagataa agataaagag aaaatagtaa taaaaaatat tgtacatatg ttaaaagaac
     1921 ttaatatcac aactgttgct gaaggcattg aatgcaagga acaagttgaa tttcttaggg
     1981 atattgggtg tgatttagtt cagggatttg tattttataa accaatgcca atattagaat
     2041 ttgaaaaaat attggataaa gagtttgttt ataatagcta aaatgtactt tataaaatat
     2101 gaactaatta agtaaaatta ttttgtactg aataaatttt tactttattt acagaataat
     2161 gattatttac agaaaagcgc gaaaagtgta ttcctttaag tgtgggatgg atagcacaaa
     2221 taggggagaa aaaatattgg ccatataaga atgcataata atataaatat ttgaaaggtc
     2281 agcattatat aaaaattaaa aagaaaataa ataatttttc tataaaaaag agactatgag
     2341 aaaatttact catagtctcc taaataatgt atttattttc ttcctgcaac ccattttaat
     2401 ccccaatgaa attcagtatc catttctcta tgacctttga agtatttaac tactttttca
     2461 acactgaatg tctcatcatt tacattttct atcaatgcta ttgaacacat attatagcca
     2521 tttttatgtt catcaagttt gacaactaag tctgaatctt gtttttgttt gattgttaca
     2581 attccatcag cttcagacca gttagctaca ccttcgtata taaaagcata aataagtatt
     2641 ctttttatat cttttatttt attaccattt attcttaaat tttctccttg tgtattactt
     2701 cctgttctgt catctccatc taattgagca aaaggtggat tgtctaaact accaaaggca
     2761 tttcctaatg cttgaacagc accttttaca ccatttttca tctcaaatag gcaacccaaa
     2821 tctaaatcta tattactgct tcttaaagag ccaaaaaaac cctttttttg tactttttga
     2881 ttccaattta aattaactaa gatttcacct aaatcgctat tttccttttt tgtaaggttt
     2941 attttttgtc ccttttttaa ttctatagcc atagtaatct tcctccacaa acatagattt
     3001 atttaaatgg tagcacttaa taagtcaata ttcaacaaga ttagaataat aatattaaaa
     3061 ttatattaaa tatactaaaa agtaggaatc acaatacctt tgtatttttc ttcaattaag
     3121 cttcttgctt tatctgatgt aagaacatct tgtagaactt taatcttttc actgtcttta
     3181 tcttcagttc taaatgctaa aagatttgca tactcgtcat ttataggagc tgtataaatt
     3241 ccgtttttct tggcatcaag accagcaggt acagcataag tagtatctat aaatgcagca
     3301 gtcacatctg gaagagaaga tggaatttga gcaatatcta actctttgaa ttgtattccc
     3361 tttggatttt ctattatgtc cttaggacta actaatccat cccctttagg taacgttaaa
     3421 agtccaatac tttcaaggta tctaaggttc ttagaacaag aagatgggtt attgcttatt
     3481 gcaattgtat ctcctttttt aaattcatca acagatttta tcttataaga gtaaagtatt
     3541 gctggacata catatagttt tttaccagcc attattttgt aacctttggc cttaacagct
     3601 tcttttagat aaggttcatg ttgaaataga tttgcatcaa tttcaccatt atttaaagct
     3661 tcatttggaa gtatgtaatc atcaaagttt ttaacttcta aagtatatcc tttttcttta
     3721 ataagtggtt ttaattcttc taataactct cctcctggaa ctaaagtagc accaacaaca
     3781 atttttttat catctttagc tttagaacat ccaaccacag ctaaagttaa tactagacat
     3841 aataacaaac ataataattt tttgaatttc atataaattc cctcctgaat ataattgatt
     3901 agttaaaaat aaaataccat tctttatcac actgcaaaat aaaaataagc ctgagtttaa
     3961 actcaggctt taaaagctaa tttagactca tctttcagta taatactgca ggatttagca
     4021 ccaatgtata tagaaaatat actggttgct gggtttcata gggccagtcc cttcaccact
     4081 ctggataaga agtatcaatt tttaattatg ttataaatat tatcatgatt ttttacgtct
     4141 gtaaacataa ttttataaat attttaaaat ctttaatgta ttttatttaa cacctaaaat
     4201 tccagaaact agaatatcag tatcagcacc taagaaatag tcatttattt caatagaatc
     4261 cagagtattt ctcaaagcat tttcttcatg ccttatacca ataagtttat tttctacaaa
     4321 tgcaacatca cattttccaa aaaaatctcc aaaaaatttt atactagtta ttatgccttt
     4381 atcaacattt aaattaaact caacatttcc accagaatat tttaattcat ttgaaagagt
     4441 atattttggt gaattaccaa aattccaatc ccaagtttcg tattttgatt tagtaagctt
     4501 ttctatttgt tttatatcat gctcactcaa acaataatta gtattatcag tatttgtttt
     4561 agaaagataa tccattatag catctttaaa ttctaatata tccatttttg aatgtaaatg
     4621 ttcacttata ttggttacac gagattttac agattttatt ccttttcctt caaattttat
     4681 aggtttaacc tttaatgctt ttgagaggtc atttatttca gatgaaaata aaagagtccc
     4741 atgatgcatt actttgtttt tgtagttgta ttgagcattt ccagaaaatt tcttgccatc
     4801 tataagaagg tcatttcttc cagaaaattc ggcattgaca tctagtgttt taagtaaatc
     4861 tattatgggc tgagtgaatc ttctgaaatc actaaaatta ttattgttac aagctataaa
     4921 tgtaaaattt atatttccca aatcatgaaa tacagctcca ccaccagact gtctcctaac
     4981 gacaggtata gaatattctt ttatatattc taaatttatt tcagaaagtg tgttttggtt
     5041 ttttccaaca attatagaag gttcattcct ccataaaata aataagtctt catcaaaatt
     5101 ttttagaaga tattcttcca ttgctaaatt aaaataaggg tttgtttttt cattataaat
     5161 taataacatt aaagtagttc ctccatagaa ttgataaaat ttttattatg aatacaatta
     5221 attttataat tcgacaataa tataaaaact cctttaaatt agaaatttta aaaactacct
     5281 tgcaaagttt aaataaataa tatatactca aaatatgttt aataaaagtg aatagaaggg
     5341 tagaggcgca ataatctatc agtactaata aggagttaag cacagagatt tattaggaaa
     5401 ggagatattg ccgaagtttt ataatcgatg ctttaagatt atattgctgg gggtacatag
     5461 aaaatatgta caactgtcac aggtttattt gtggtgagct atcatttaag gaattatatt
     5521 atatttattt ttactattgt tgatatattg taatgtttaa gccttgagtg tttaccactc
     5581 aaggcttttt tagttttatt gtaacataat accctgtaca gttcacaaaa ttaaaaggag
     5641 agggagattt atatgaggaa caaaaaaatt aatattattt ttctgacgac aatcatgttc
     5701 ataatgtcaa cagtaatggt ttttgcagaa gaagatatag acactattgc actagccaat
     5761 gcagaaaaat ttgggatttt aactttaata ccaccattag ttgctataat acttgcattt
     5821 ataactaaaa acgtaataat ttctttactt ataggtattc tttctggtag ttttataatt
     5881 aaagctagtg gtattaatgt ttttgcgaca tttatacagg catttttaga tttagtagac
     5941 agagctttag tatctttagc agacccttgg aatgcaggga taatactaca ggttttggca
     6001 attggaggag taataaatct tgttgctaaa atgggaggag ctaaagctat agctgaagca
     6061 cttgctaaaa gagcaaagtc agcaaaaggg acacagctta taacatggtt tttggggctt
     6121 ttagtattct ttgatgatta tgccaattca ctaatagtag gtcctatgat gagacctgtt
     6181 gcagataaaa tgaaaatatc gagagaaaag ctggcattta taatagatgc cacagcagca
     6241 ccagttgctg gtttagctat aatatctaca tggataggtc ttgaggttgg tttaatacat
     6301 gatgcttttg aaagtataag tatagatgta gatgcatttg gtatattttt aaatacaata
     6361 ccatttagat tttacaatat attaatatta gctttcattg taatatcagc attattatta
     6421 aaagaatttg gtccaatgag aaaagctgag ataaaatcta gaagtagaaa aattagtata
     6481 gaccttgatg aaggcgtaga agagttggat gatttagctc caaaaaatgg agtgaaattg
     6541 agtgtatgga atgctataat cccaatagga acattgataa tagttgcatt agcatcattt
     6601 tattatagtg gatatacatc tataatggga ggagacgata aggctctgat acaactattt
     6661 acaaattctc catattcttt tgaagcaata aaagaagcat ttagtgcatc tgatgcatct
     6721 agagcattat tccagtctgc acttgttgca agtttagttg ctattataat ggctgtagta
     6781 aaaaagattt ttacaatatc agaagcaatt gatgtttgga tagatggaat gaaaagttta
     6841 gtaatcactg gtgtaatatt aatacttgca tggtcattaa gttcagtaat aaaagagtta
     6901 ggtacagcta aatttttaat acacctatta tcaggttcac taccaccatt cttattacca
     6961 agtttaatat ttggtttagg agcaataata tcttttgcta ctggaacagc ttatggaaca
     7021 atgggtatac ttatgccact tgcaattcca ttagcatact cattaaatcc tgatatgtct
     7081 tatgtaatag taagtactag tgcagtttta acaggtgcaa tatttggaga ccattgttct
     7141 ccaatttcag atacaacaat actttcttca atgggagctg gctgtaatca tatagaccat
     7201 gttaacactc aaatgccata tgctatattt acagcagtaa tcactattgt atttgggtat
     7261 ataccagctg gacttggatt gccaatatat atagtattac cagtggcaat agctgctata
     7321 tttgtaggta ttcaaataat tggtaagaaa gttgatgaag cggaaataga acttgtagaa
     7381 taaagatata aataagtttc accttttatt atcactattt tgttagtttt tagataattt
     7441 taaaaagtta ttgaaaataa tggcgaaata tagtaaaata aaagagtgat aagcggatgt
     7501 ggcggaattg gcagacgcac tagacttagg atctagcgcc attggcgtgg gggttcgact
     7561 cccttcatcc gcactttaca gtacttaatt gtactgaaaa aaactggggt tgctggcttt
     7621 cggagtgggc tatcccgttt ttattttttt gtatatataa tagattggca ctttagtata
     7681 aggtgctttt tttataggca aaaacagtac atttatttct gttttattat cattcatcta
     7741 ttagaatata ctttagttaa gaatgtgtta ataagcagtg ttcacattct tgataaaatg
     7801 tggaataata tgtattttta ctaagacgga aatgaaataa ttaaaccatt aaataatatt
     7861 ataagatgat gaataagtag ataaatcaaa ttttgtagga ggatattatg gaaaaatata
     7921 tatggttgtt tccattactt tttatttttc atgatatgga agagataata ggttttggaa
     7981 tatggcttaa aaaaaataaa tcaatgcttg ataagaagta tccatttatc agtaaagttt
     8041 atgaaaatta tagtactgaa ggcatggcat ttgctgtgtt tgaagaattt attttatgca
     8101 ttattttctg tatacttaca gttataacag agaatcagta tgtatatcta ctatggttag
     8161 gtagttttat agcatatacc ttacatttgg taattcatat agggcagtca atcataataa
     8221 gaaaatatat accttcactt attaccagta tcatatgctt accaataagc atttggtgta
     8281 taagtaagag tatttatata gttgattgtg aaatgagtac aacaattctg tacagtataa
     8341 ttggcattat aattgtagca ttgaatttaa agtttgcaca gtcgctgatt ggaaaattta
     8401 caaagtggat gtcaaacata taaattttag tttattggac agttcaagga atcagaaatt
     8461 ggaattttat agtattaata ttttatagta ttaatataat gtataaaatt ttatagcaat
     8521 gtatttcata agtataaagt gactagaatt ttaagaaata tacaaattct ataaaaacta
     8581 aataatttca agtatatttt aaaaatctta tttttcacaa gtttaaaatt gtaaacgatt
     8641 tcatcaagtc tatgaatata cttttaacat gttatagtaa atctcaatat aaatttacat
     8701 ttggttcttt gtgtgttatt ttaataataa attaagtgaa aatgttattt tacattaata
     8761 tataatatct ttgcataaga gtgaataata taaaagagaa ataattgata ataaaaactt
     8821 aacaaaaaaa ttaatacata aaaaaaaata aatataatgt gttgacattt aattgaaata
     8881 ttcatacaat aaaattgaaa ataaatattt ccaaatgaga ttagcgagag agtcctttat
     8941 acgaaaaggc gccgaagaag aaatttaaga aatgccaatt tttagagaag ctttcaggca
     9001 aaaatatcgc taattgatgg gactctggag agacttaagt taagatacta taagtcaccg
     9061 aagaagcaaa ataatattaa attattaaat ctttcaggta tacaaacagg gatatatagg
     9121 ctaggattat tttccatgct gatatatctt ttttttatga aaaagtacag gaaataatat
     9181 caaagttact taaatacagt aggtctaatt ttctagggag ggttatatat gaatgaattg
     9241 aaaagagtat ctttgtacaa tatacacaaa gagcttggag caaaacttgt tgaatttgct
     9301 ggttgggaaa tgcctttaga atatgagggt ataaataaag agcatgagaa ggtcagaaaa
     9361 agtgcaggta tatttgatgt atctcatatg ggagaggtac agataaaggg agcagagtca
     9421 gaaaaattta tacaaaacct agttacaaat gatataagca cattaaagat aaatgacatt
     9481 atatatacac ctatgtgtta tgaaaatggt ggagtagttg atgatttatt aatatataaa
     9541 tttggtgaag aagattattt attagtaata aatgcaggta atatagataa agatgtagca
     9601 tggataataa aacaaagtga aggatacaat gtagatataa aaaatatatc tagtgaagtg
     9661 agtcaattag caattcaagg acctaaagca gaagaaattc ttcaaaaaat cacagatata
     9721 gacttaaact caataaaatt ttataagtca attccatcaa caaaagtatg tggttgtcct
     9781 tgtttagttt caagaacagg atatactggg gaagatgggt ttgagatata ctgtaaaaat
     9841 aaatatgtag aaataatttg gaatgaagtt ctaaaggttg gaggagaaga tatatgtcca
     9901 gctggtctag gttgtaggga tactttgaga tttgaagcag ctctacctct atatggacat
     9961 gaaataaatg aacacatatc tccaattgaa ggtggtttat ctatctttgt gaaaacaaat
    10021 aaagaatcat ttattggcaa atcaatttta tcaaaagaaa aagaaagtgg agctaaaaga
    10081 aagttagtag gatttgaaat gcaaggtaaa ggaatgccta gaaatggata tgatataagg
    10141 attggtgata aaacagttgg atttgtaaca acaggatgtg catctccaac gacaggaaaa
    10201 attttaggta tgggaattat agattctgaa tatgcaaaag taggaaatga aataggtata
    10261 gcaatcagaa aaaaagttgt tcctgctgtg atagttaaga aaccttttta taaaaaacaa
    10321 tataaaaaag ataatattat attaaacaaa gaaaataaat tttcatatat acctgctaca
    10381 agtgaagata aatcaaaaat gttaaaagta gttggattaa attcagttga tgaattattt
    10441 tcagatatac cagaagaagt aaaattaaaa agagatttaa atttggaaat tggaaaatct
    10501 gaattagagg taagcaaaat agtaaaaaga ttgtcagaag aaaatttaag tttagaagac
    10561 ttgacttgtt ttcttggtgc tggtgcatat gaccattata taccatcaat tataaaacat
    10621 ataacttcaa gatctgaatt ttatacagca tatactccat atcaagctga aataagtcag
    10681 ggaactttac aagtagtttt tgaatttcaa tctatgattg cagaaattac aggtatggaa
    10741 attgcaaatg catcaatgta tgatggagca actgctgcaa tagaagcatg tataatggca
    10801 atgaatcaga ctagaaaaag taaaatagta gtgtcaaaaa ctattcatcc tgaaactctg
    10861 tcagtattaa gaacttactt acaatataaa gattgtgaga tagtagaaat tgacttttgt
    10921 aatgaatatg ggactacaga tatagaaaaa ttaaaagctt ctgtagacaa agatacagca
    10981 tgtgttctta tacaaactcc taatttcttt ggaattattg aagaaatgga agaaatagaa
    11041 aagataactc atgaaaataa ggctatgtta attatgagtg tagacccaat atcattagga
    11101 gttttaaaaa caccaggtga gataggtgca gatattgttg ttggagaagc tcaatcactt
    11161 ggaaaccctc ttaattttgg aggtccttat gtaggatttt tagctagcaa atctaaatat
    11221 actagaaaaa tgccaggaag aatagttggg caaagtttag atgtagaagg taaaattgca
    11281 tatgtattaa ctttacaaac tagagaacag catgtaagaa gagaaaaagc aacttctaat
    11341 atatgttcaa atcaagcttt aaatgctctt gtagcttcaa tatatatggc tactatgggt
    11401 aaagaaggat ttaaagaggt tggtatgcag tctatgaaaa aagcacatta tacctacaat
    11461 aaacttgtac aaactggaaa atataagcca atatttaaag gtaagttttt caaagaattt
    11521 gctgtacaag gaaatcttaa tatagaaact ataaatgata aactcttaga agaaaatata
    11581 ttaggtggtt ataatttaga atacaattat ccagaattaa aaaattcaac tttgctttgt
    11641 gtgactgaga aaagaagcaa agaggaaata gataagttag ttggaataat ggagggatta
    11701 taatgaaaga atacaatagt ttgttgatag atatatctaa aaaaggtagg aaagcatact
    11761 cacttccaaa gttagatata gatgatataa aaatagaaga tatgattgac ccagatatgg
    11821 caagacaaag tgagttgaat ttgccagaag ttggtgaatt agaattagtt agacattata
    11881 cattattatc aaataaaaac tttggagttg atacaggttt ttatcctttg ggttcttgta
    11941 ctatgaaata taatcctaaa ataaatgaag atatggcagc acttccaaac tttactggaa
    12001 tgcatccata ccaatcttcg gatacagctc aaggttctct ttctttgatg tatgatttat
    12061 caagaagact tgcagaaatt acaggtatgg atgaagttac actacaacct tcagcaggtt
    12121 ctcatggtga gtttacaggg cttatgatta taaaagcata tcatgaaaat agaggtgacc
    12181 ataagagaac caaagtgata attccagatt ctgcacatgg aacaaatcca gcaagtgcag
    12241 ctatggcaaa ctttgatgtg attcaaatag catctgataa aaatggagct gtagacataa
    12301 atgtattaaa agaggttttg aatgatgaag tagcagctct tatgcttact aatccaagta
    12361 ctcttggttt atttgaaaaa aatataaaag aaatagctac tcttgtacat gaagcaggag
    12421 gacttttgta ctatgatggt gcaaacatga acgctattat ggggataaca agacctggag
    12481 atatggggtt tgatgtggtt catcttaatc ttcataagac tttttcaact cctcatggag
    12541 gtggaggtcc aggagctggt cctgttggag ttaaaaaaga attagtacct tttttaccaa
    12601 taccagtaat tgaaagaaaa gaagataagt atgtactaaa ttatgataga gaaaaatcta
    12661 ttgggaaaat taaaaatttc tatggtaatt ttggagttct tgtaagagct tacacatata
    12721 tactaactat gggaagggat ggacttaaag aggctagtga aatggcagtt ttaaatgcta
    12781 attatataaa ggaaagtata aaagatgatt atattttgcc aatagatacc ttgtgtaagc
    12841 atgagtttgt attaggtgga ttgccaagag gagaggccaa tataaaaact atcgatattg
    12901 caaaaagact actagattat ggataccatc ctccaacaat gtactttcct cttattataa
    12961 atgaggcact tatgatagaa cctacagaaa gtgagagtat agagacatta gatagtttta
    13021 tagaagctat gaaaagtgtt gctaaagagg caaaggaaga accagaacta ttgaaaactg
    13081 ctccacataa tactttggtc aaaagagtag atgatgcaag ggcagtaaag aaacctatac
    13141 taacatggtc acaaagataa atagttaaaa agtagagatt taaagtataa agagaattaa
    13201 atttataaga aaaagctatc taacttaatc tagtttagat agcttttttg tatattagta
    13261 tatatggtat ttcctatata aagttttaaa ttatattagt gcttatatag aatattgata
    13321 tgatgattat gtatagatat taataagaat tgttgaattt atcttgcctt aactttattt
    13381 actcttttat attaagaaaa acttaactaa aatatagcga aattcataaa aatttaggaa
    13441 atcttaagaa ttataatatt taaatcttta gatttatatt ttattatata aactatagaa
    13501 taataaaata aaaaaattga gaattttata atatatttgt agatttgagt ataaaaaagt
    13561 gctaagttga aaggagtaaa atatggataa ggttaattta gaaaggtata gttgtgatat
    13621 aaatgttggt ttaaataaaa atcaacttca aagtagaata gatgataatt taattaatgc
    13681 atttgataat ggaaaaacca aaacatataa acaaattttt aaagacaata tatttacact
    13741 ttttaatctc ataaatattg ttttagtctg tttattagtt tcagttggtt cttttaaaaa
    13801 taccttattt atatttattg ctgtaattaa tactattata ggagtaatac aagagataag
    13861 agctaaaaga ttattggaca atctatctgt tatagtttca aataaggtat ctgtcataag
    13921 agaaaaagag aaaaaagaga ttgatgtaca agaattagta ttagatgata ttatgatttt
    13981 aaaaacaggt aatcaaattt gtgcagattc aaaagtttta aatggaaaat tagaggtaaa
    14041 tgagtccttg gttacaggtg aatctgatat tattataaaa aataaaggcg attttttata
    14101 ttctggaagt tttgtagttt ctggagaagc ttttgtaaga gttgaaaata taggaaaaga
    14161 taactatgca aataagatag ctaatgatgc aaagatttct aaaaaacata attctcaact
    14221 tagaaattca ttaaatgcaa tattaaaaat tgtaggtata ataataattc ctctaggaat
    14281 tatattgttt gcaaaacaac attttggaaa tggagattct atagctacat ctgtagtagg
    14341 tacagtggca gctgtaaatg gtatgatacc agagggatta acattactta cgagcattgc
    14401 tctagctgta ggaactataa aacttgctag tcataaaact ttagtgcaag aactgtactg
    14461 tgtagagact cttgctagag ttgatacact atgtcttgat aagacaggta caattacaga
    14521 aggtaaaatg caagttgatg atatagtaat gttggaagaa gtggatatta atgaaataat
    14581 ggggaatata tgtaattctc tcaaagatga taatgcaaca ctaaatgcaa taagagataa
    14641 gtataatgtt ttagatacgt ataaagctaa gaatttaata ccattttctt cagaaagaaa
    14701 atacagtggg gttacctttg aaaataaagg tacatattta ttaggtgcat ttgagtttat
    14761 atttaaggaa aaaaataaaa aacttaggat agaagttgaa agatattcta aaaaaggaaa
    14821 cagagtaata gttttagctc atagtgattc ttatatggaa gatgataata ttcctgaaga
    14881 tataaagcca ctagctttta tattgatatt ggataaaatt agagatgaag ctaaggaaac
    14941 tttagaattt ttttacaatg aaggtgtaga tataaaaatt atatctggtg acaatcctat
    15001 aacagtaagt gaggtagcta gaaaagcagg tcttaaaaca gctagtaatt ttattgatgc
    15061 aacaacactt aaaaatgata gtgatatata caaagcagtt gataaataca gtgtttttgg
    15121 aagggtaagc ccacaacaga aaaaacaaat gattttagca ttaaaaaaac aaaatcatac
    15181 agtagctatg actggagatg gtgtaaatga tgtgctagca cttaaagaag cggattgtag
    15241 cattgccatg gcatctggaa gtgatgttac taagaatgtg tctaatttag tcttattaga
    15301 ttcaaacttt gcatcaatgc caaaggttgt tatggaggga agaaaagtaa ttaataacat
    15361 tcaaagagca tcttctttgt ttttagtaaa gacaattttt tcattcttct tgtcgttatt
    15421 aacattattc ttcttcagta gacagtatcc atttgttcct atacaattat cactcattgg
    15481 ggctgtaaca gtgggtatac catcattctt cttagctctt gaagcaaaca aaagtagaat
    15541 ttcaggaaat tttttactca atatattaaa aaatgcttta ccaggagcac tatgtgtgac
    15601 agtaaatgta attattgttt acaatatagt tgaatatttg ggttatagtt caagtgtatt
    15661 ttccacaatg tgtgctatat taactggtgt atgtggtctt ttaatattaa gacaacaatg
    15721 tttgccattt aataaagaaa gattattttt aatagtaagt atgactattg catttgttat
    15781 tgggattcta tttttaggtg gattattctc atttgtagca ttgagtagag aattaattct
    15841 cataactatg gcattagcaa tattaatgcc aattatgata aaagttatgt tatttgtctt
    15901 agatgagata ttagagacaa ttgttccaga tttaaattaa ttttaaaatg atataaataa
    15961 gagcagtttt agttgaactg ctctttttag atgtgtttaa ttttatgtaa gaatatgtat
    16021 ttttattaat cagctatata tgagcaacag tagtcaacaa cttcattaac ctttaagtca
    16081 aaacttgaat cactaggaac attaaatttt tgtccttctt tgtaagttac aaactcattg
    16141 cttccaggca gttttacatc catacttcca gcaagcatct ccattatttc ttcttctcta
    16201 gtagaaaaag tgtattctcc tggcaacatt aatcctaaag tttttctttc tccattaggc
    16261 agtattatta ctctgctact taccttacca tcaaaataaa cgtttgcctt ctttactgca
    16321 gtaacattct taaattcact cattagataa aacctccaaa attatttgta catatatatt
    16381 atataaaaat aatgtaaaaa atgctatata tttacaaaaa aatttagtat ttattaaaaa
    16441 aattaataag aataaaaaag agatggaatt gcttaaattg ataaaaaata taaaataaat
    16501 tgcaaagtaa gcaagaaaag aataagttga agagttatat attataaata ttaaaaattt
    16561 tagtattaat aaaaaagata ttacattaat atgaaaatat aggagattag aagtgtttga
    16621 taattacagt acatgttaga tatatcattg taaattaaca tagctttgaa tataataatg
    16681 ttcaaaaggc aatatttttg aggtctgaat ccaataataa tatagtctta tgattgtaga
    16741 tatcaaaaaa tataagttac taagaatacg ttttgtagat ttatatatga ttaataacta
    16801 aagct
//
LOCUS       DAEOSP010000068        16797 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.068, whole genome shotgun sequence.
ACCESSION   DAEOSP010000068 DAEOSP010000000
VERSION     DAEOSP010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16797
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.068"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            393..1796
                     /locus_tag="KRH54_001835"
     CDS             393..1796
                     /locus_tag="KRH54_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG8579497.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            complement(2273..3295)
                     /locus_tag="KRH54_001836"
     CDS             complement(2273..3295)
                     /locus_tag="KRH54_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG8579498.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(3330..4928)
                     /locus_tag="KRH54_001837"
     CDS             complement(3330..4928)
                     /locus_tag="KRH54_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBG8579499.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            complement(4970..6235)
                     /locus_tag="KRH54_001838"
     CDS             complement(4970..6235)
                     /locus_tag="KRH54_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8579500.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            complement(6379..7791)
                     /locus_tag="KRH54_001839"
     CDS             complement(6379..7791)
                     /locus_tag="KRH54_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBG8579501.1"
                     /translation="MASTNKNNATSKEEEKEVSTSVLESCTVKEKKAKMNQTNFSNIN
                     ENQTNENKTNTYKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTEKNKKVIF
                     KGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDYIMKIT
                     HNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIW
                     SNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDN
                     LWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEM
                     MLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYE
                     VERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKE
                     EWITFDGMDVLKQMYMGVEE"
     gene            complement(7829..8836)
                     /locus_tag="KRH54_001840"
     CDS             complement(7829..8836)
                     /locus_tag="KRH54_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG8579502.1"
                     /translation="MGKYQYSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            complement(9033..10355)
                     /locus_tag="KRH54_001841"
     CDS             complement(9033..10355)
                     /locus_tag="KRH54_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579503.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKLDISRL
                     SIDTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIVVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            complement(10713..12353)
                     /locus_tag="KRH54_001842"
     CDS             complement(10713..12353)
                     /locus_tag="KRH54_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG8579504.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHVSHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            complement(12458..13114)
                     /locus_tag="KRH54_001843"
     CDS             complement(12458..13114)
                     /locus_tag="KRH54_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579505.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            complement(13140..13841)
                     /locus_tag="KRH54_001844"
     CDS             complement(13140..13841)
                     /locus_tag="KRH54_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG8579506.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILL"
     gene            14649..15128
                     /locus_tag="KRH54_001845"
     CDS             14649..15128
                     /locus_tag="KRH54_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG8579507.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            15125..15904
                     /locus_tag="KRH54_001846"
     CDS             15125..15904
                     /locus_tag="KRH54_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579508.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNKFKFLTWKQDEVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(16154..16654)
                     /locus_tag="KRH54_001847"
     CDS             complement(16154..16654)
                     /locus_tag="KRH54_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8579509.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSNLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
BASE COUNT         5850 a         3012 c         1993 g         5942 t
ORIGIN      
        1 tataattata ttctaatata ctacctacta ctttgcgcta caacaatttc tctatttata
       61 tgatttgtaa aatatagtac aacatttttt atcattttca tttcaactaa atttgtaata
      121 ttttattcaa tacttaataa atacttaatc aatacatgct ttataatact ttactcaata
      181 tataatcaat acataattta taatttgtaa tactttattc aatacataag tagaaatctc
      241 tatatacata aattcatttt ttcatacatc ttacaaattt atttttattt agataacatg
      301 tatatttttt ccatgaaaaa taaataataa aaaatagtaa atatggaaaa tataaaattg
      361 attaacaata attaattacc tgaggtgtga atatgaaaat gctaaacaac ttttttaaga
      421 aaatcaacaa tccagctcct gtctttgcac agacaaaaat ggaaagagat aatcaaacag
      481 atgattcact ttctacaaaa ccaaagacta catttagaga cgttgctgga ttagacgaag
      541 taaaagaaga actatttgaa atagtcgatt ttatgaagtc accacaaaaa tatcaaaaaa
      601 tgggtgcaaa aatacctaaa ggagtattat tttatggtcc tccaggaaca ggtaaaaccc
      661 tacttgcatc tgctgtagct ggtgaaacaa actcatcatt tttcaatgtt actggttcag
      721 agttcgttga aaaatatgta ggtgtgggag caaaaagagt tagaactcta tttgaaaaag
      781 ctagaaaaga agctccaagt ataatattta tagacgaaat agatgctgta ggagcaaaga
      841 gacatcttga aagtaacaac gaaaaagacc aaacactaaa ccaactttta gttgaaatgg
      901 atggatttaa taaagattcc aatgtattaa taataggtgc taccaataga cttgacttat
      961 tagatgaagc tttacttaga ccaggaagat ttgacagaca cattcatata ggtgcaccaa
     1021 actatcacac tagatttgaa attctaaagg tacatacaga tgacaaacct atagacaaat
     1081 ctgtaaatct agaactttta gctaaaaaaa ctcatggttt caatggggct catctttcaa
     1141 atatagcaaa tgaagcagct atatttgcag ttagagatga cagtgagtgt attacatcag
     1201 aacattttga caaggcactt gaaagagtta ttgcagggct agaatctaaa aactctgcat
     1261 tggttgaaaa agagaaaaaa atagtagcat atcatgaagc tggacatgct cttgtaagtg
     1321 atatagtagg aatttgtcct atacaaaaaa tatctatagt tcctagagga caagcattag
     1381 gttatgttct tcaattacct gatgaagata gatatattta tactaaggat gagttgattg
     1441 gaaaaataaa aatattactt gctggaaagg cttcagaaga attaatattt aatcataaat
     1501 ctactggtgc aaaggacgac ttaaagaaag tcactgaaat agctaaccaa atggtttgtg
     1561 aatatggtat gagtaactta ggatttatga ctattgatgg aaatgataag actttcttat
     1621 gtgataaggt tcaaaaggaa gcaaatcgta tagttgaaat ttgttataaa gagactttag
     1681 agatgttaaa ggataatctt gaagatttac attctgtttc taaattctta tttgaaaaag
     1741 aaactatgac tcatgaagag ttaaaagatt tgataggaaa agaagctgta aactaatata
     1801 gttttaaaaa tttgaggctg tctcaaaata gagataagcc tctttttatt gttaaagtta
     1861 taaaaataca tttgatttta tacaaaaaag tgcctcataa actaaaaatt tcattttgag
     1921 acacttacat attttaatta catattttaa ttttgtctat ttacagaata ttatatctac
     1981 ataaaactaa actgctgtaa acttaactac ctccagtaat gtttgaagtg gtataaaagc
     2041 tagtagccta gtttctacac ttataattta tattttacat aactaaattt agtagatttt
     2101 tatactcaca atttagaacg attgaaaact taaaaatttc ttatcagata tctacaactt
     2161 aattaggtat ctatcacttg attaaatatc tatcacttaa ttaggtatct atcacttgat
     2221 taaatatctg ccatttaatt aaatatctac cacttaatta aatatctacc acttaaagtg
     2281 tagaacctct ttcaatcaat tcaggtttta acatttcaac gactgactct ggattttctt
     2341 catttaataa ggacttaagt atgttaattg ttttttcaat caacaaatct agtggctgtc
     2401 taatcgtcgt taaattataa ctcttccatg aagccatttg aatatcatca tatcctatca
     2461 ctgataaatc ctctggcacc ctcaacttgt actcaaacct tgcagcatcc attacaccaa
     2521 tagctatcaa gtcacttgtg caaaaaattg ctgttggagg atttgataat gctaacattc
     2581 tcttacctgc ttcatatcca gaatcataat catagtttgc agattcttct ttaaggttat
     2641 gaatcccact ttgtcttaac ttagagtaaa atccaatttt cttttccatt tcttcacttg
     2701 tttctctcgt aaattgaata tatcctatat tcttatgacc tttcttaagc agatattcag
     2761 cagccattcc cccaccttca attgggtcta catagactgt acttattttt ataccaggta
     2821 taaaacgatt aaaaagaatc acaggaatat tgttttcttc acaagtctca gccatcacct
     2881 tattcatggc cgaagctgta ataatcacag cttctacttg aaactgaatt acttttgaaa
     2941 ttatactatc tatactttgt tgtctaggaa ctttaaaaac gagacactgt ttacctatct
     3001 cttgaatttt ttctacaaat aaattgataa ttctattgta aaatggacct agactatcac
     3061 caactatcaa tcctataaca ttagtttttc cactaatcat tccccgtgct accaaattag
     3121 gaacatatcc tatttcattt gctacacgaa aaattctttc tcttacttca ggttttacgc
     3181 cctttccatt tgcactacca aatgctcttg aaacagatga ttgagatact cctgccaatc
     3241 ttgcaacatc ttttgaggta gccgatttat tataatgaac tttcttttga ttcatttagt
     3301 atttctcctt aaatatctag tatttcttct taaaattgta tcaaaaataa tataatatat
     3361 tggataataa aaaaagctaa tacaattaat tcttgtttac ctaaagaaaa tggcactata
     3421 gatactccat ctactttatc cattccttga gaaataaggt gatttctcat accaaatgca
     3481 gtgtattttt taatatcatc tgcattatga tttttcttta aaatgacact aaaaatatat
     3541 ccaaatatca atgttattgt agcacctatt gctggattcc acaaccaact cacattatat
     3601 ttattagaaa taaaaaatcc taaaactaac cctacaataa aaccaataga tactccttta
     3661 tcattagctt tatatgtaaa cattgctagt acaaatgcac ctgtagctgg tccagcaaag
     3721 tatgagatat agttaccaac aaggtcaaga attgatttga tgcttcctcc aaatcccata
     3781 ataataaaac ctgtcataac aactcctata actacagtta taatcattgt aacttttaat
     3841 gatgattctt tgttatcttt tttgaaataa aactcataaa tatccttcgt aaaaacagtt
     3901 gtcatactgt ttaatataga gtcgatacta gacattgctg ctgcaaacac acctgcaata
     3961 ataattccaa tggctcctac tggaagttca tttaaaataa aagtaatcat tatttcattt
     4021 gaagtttcaa atgtccttcc cccataaaaa acaaataaaa ttactcctac caatagcata
     4081 aagtagtaaa ctaaattcat aacaaatgca ctaactacaa aagaattttt tgcacatttt
     4141 aaagatttag atgttaaaac tctttgtact tgagattggt caaaacaaaa gtatcttatc
     4201 cacattacag ttccaccaat taaagtagcc caaaaagtat ttgtcactga aatatcccta
     4261 ctgaaatcaa atgtatttaa ttttccactc tgttgtgcaa tttgtaatgt atcaaagaaa
     4321 cttagattca ttccttttaa ggctacaata attactataa atacagcact tattactact
     4381 atcatttgaa tactatcagt ccatataact gccttaatcc cacctaatag agtgtatata
     4441 attgatacaa gtacaactat tgggactaaa gcataaagtg accagccagt aatcatttga
     4501 ataataagca ctggaatata taccatagaa ctagcttgaa tcaatgaatt aataaaaaat
     4561 tgaataatag ttaatcctct tgaataattt cctaaacgaa gtcccatata ttcatatata
     4621 gaggttattt ttaatttata gataactggg gtggtaatgc tcagtgcaaa aaatattgct
     4681 aaaggtactg taatattaac cattactgga tacattccat ctacatatgc ccatccagga
     4741 ccacctataa agccatttgc actaatcata gtagctgcaa ctgataaagc aattggaatc
     4801 catggcattg acctacctgc caagaaataa tcttcttgat ttttattatc ttttcctgaa
     4861 taataaccaa taactaacat taatagcaaa tatcctatta taatacatac atcaattgat
     4921 gaaatcataa gccacccctt tctaagtaac aaaataaatt cctagtattt tattttttat
     4981 tatttaacgt ctccggtaag aataaccata caatagcacc tgctacaaag actgcgattg
     5041 atatgtaaaa gatagtcatc atacctaaag taccagaagc tgtaccagct atagcaggta
     5101 ttgcaaacgt agatataatc cttcctccat taaatgacat accagatgac aacgaacgat
     5161 atttacttgg gaacaactct gtgtagtatg ctccccatgc tccagaatat ccaacagcaa
     5221 cacctagtaa tactgatatc atatagaatg ttgttgtatt atctccacct aattttgtat
     5281 atataaaaac aaatactgct gctaatgcca tacctataaa aggtattttt cttccaaatt
     5341 tatctgcaat tgaaccccag aataaatatc ctactaattg accaattcct tgtaaactat
     5401 atatagaccc agccactgct gctgacattc ctattcctgt cactaggtat gtagttgcat
     5461 tatttgaaaa tgctgaatat gctgtaaaat ttgcacctga taaaagaatc gttcccaatc
     5521 caatccataa gtattttccc ttatacatac ttatatagct aatcttttca ccagtttctt
     5581 ttcccttttt aactaattcc tttgtatact ccaaatcatc tggcacaaag aagattgcta
     5641 agatagctcc aactataggt ggtataatcg cagtatacat caatatttgc caatttgcat
     5701 cacctaaaac gccatatact ttcccaccaa aaatcattgc taatgaaaat aaagcagtta
     5761 atatagcagc taacttacca cgatatttac ttggaaacat atctgcaact aaaggcatag
     5821 ctgttccaaa tacgcctccc aacactactc ctgtacacaa acgtgctatt ttccataaca
     5881 taatattggt aggagataac attggtataa atgttgtcaa tccaatcagt aaagcaacgc
     5941 taattaaagt cctcttcttc ccaatttttg tagaaatcac cccaaaaata aatgtacctg
     6001 gaattgtacc aaaagacata actgcaaata tttcttctgt ttgttttaat gaaaaaccaa
     6061 atcccccatc tgctaaagca taaatcattg ttggtttaat aaaagtaccc accataaaat
     6121 tgtagcaata aaaaaagtat attaaagcaa ttgaaaaaaa tgcaattcct ctttttaggt
     6181 ttgaatagtc ttttcctaat atgatatcat tttctatttc aaccgttgtt ttcatatttt
     6241 tataattccc ccttttaaat ttagtcataa ttttgctaaa agtttaaaca tctatttttc
     6301 atatacattc cccacttaaa ttttataaag caatacttcc ctatatacat ttcaaaacta
     6361 tttatactct aaacagcttt attcttctac tcccatatac atttgtttta atacatccat
     6421 accatcaaaa gttatccact cctcctttac tttaccttgt agtatatgat aatgattgat
     6481 tcccattact tctatatgct ttccagaagg ctgtccaaaa aatcctatac cttcatttat
     6541 tccacgtaat ctccatctta cagatacatc ataaccttca tttttttctc tttgattaca
     6601 agttactctt tccacttcat aacttccatt tggaattgat gcaagtaaac ttacaatcat
     6661 tccttgaatc tcatcatagc catttaaatc tttatcacat ataaaatgta ctactgcatt
     6721 atcatgatag tattttttaa cttcatttat atatttataa ttataaatac ggcttgtcat
     6781 ttcaagcatc atttcgccaa cagaatcatc ttttgcttga tacttagttg gcataaattg
     6841 accatccata gattcacaga taccataagt tgattgtaaa ctaagcactt tagattcact
     6901 tgcctttgcc atactctttg ctacttcctg agggtttaat cctaattgag taacaatcca
     6961 taaattatct ctaaccaacc attcttcgtg gattctattg ttagttgtgg cacaatcaat
     7021 tacagttcta aagtttattt ttctacctgt tgctggtcca aaattactat cacctaagtt
     7081 agttgctgta gacaaaacac ggtgagaaga taaaaaacca tcagcaccaa agttagacca
     7141 tattacattt tgaccaatta atcttctatc aggaaacgca tgtaatgttt gaagagtatt
     7201 ggaaataaca tctttaatac caactaaatt tgaagagcca caatgcattg taacattatt
     7261 gtgatatgta tcataaataa cacctatacc tttttcttcc cagatattat gtgtaatctt
     7321 cataatataa tctacaaaat ctctatactt agaatcaaat ccaggtattt cctgttccct
     7381 ttttgtctgt tttgaaaaat cattgtaatc cattactcca acaggcgtta cttctaatga
     7441 atctccatag taaatttttg tagatgacat attatcttcc cccttaaata taactttttt
     7501 atttttttca gtaagagtat ttgtatttaa tttatctgta tccactgtat ctatgttaac
     7561 tttatttatg tcggatttat ttgtgtttgt tttatctata tttcctttat ctatatttcc
     7621 tttatatgtg ttcgttttat tctcatttgt ttgattttca tttatattac taaaattagt
     7681 ttgattcatt ttagcttttt tttctttaac cgtacatgat tcaagcacac ttgtagaaac
     7741 ttctttttct tcctctttac ttgttgcatt atttttgttg gtagatgcca ctttatatcc
     7801 ccctttgttt tagaatacgt atgcataact aagcattaaa aatactttta gttctctcaa
     7861 gaatatcaag cccttgttgt ttcaaccaat aaggaatatc aattaacacc cagttttctt
     7921 gaagtttatc gcccttacga taataaacat caacaacttg catatctgct ttaacctctc
     7981 cacctggtaa tcctaaaaaa cctccacatg gtacattagt cagattaggc catccaaaga
     8041 aacaagcaaa attaccttct gcaaatctac atacatgccc attgaaagtc tttcctttta
     8101 aattatttct aaatggtagc tggtgttgct cttgatatct aggtatagta taagaagcac
     8161 caattccacc tggaccatac caaatcatat ctttagccca agatttttca agtatttctg
     8221 gtgggcatcc catagcacca ctttcattta attcagataa atcatctacc attttattta
     8281 ctaatgctaa agtttttact ccttcaactt catctgcatc ttcaaaaagt aatccattat
     8341 ggtcaattgg ccctggatat acaaaatatg aacctgtttg tggtggcaat ggattaactc
     8401 ctgcttgaat cataaaccca attatatcta caaataatcc agttttagca attttaccat
     8461 cttgaacaca gttaaattct gcatatctta aactaatcat tttacgagta tgtttaatgc
     8521 caagccaatc gttatcaaaa agtcccataa aatgccccat actcattacc cattcactat
     8581 tgtcaatctc atttgttcct gcaatgaaaa tatcttgtcg tctttgcata ttactcaatg
     8641 atttttttaa aggcttccaa aatacatctg ctacattttc agtgccttct tgttcacgaa
     8701 atggatacac acctttccag ttataatctg atttcatatg ttgctttaag acattttcaa
     8761 cctcatcccc agtagcattt tctaatgctt caaaataatt tcttacaact ttttttgaat
     8821 actgatattt tcccataatt atctcctccc agatttattt aagttataaa ttaactttct
     8881 catttcatgc atacgatttc ctttttaatg atttttcagc tgttttaagt ttattttatg
     8941 aatacatatg catttttcta aatattaaca tatatctaaa aaattgtaaa gaaaatgtta
     9001 tcataaaaat ttaatttttg acttatatct atttaaaaca acagaaaagg tttaaaacgt
     9061 aatattctac tgggtctacc atacttgctc ataagctgtt cactcactac ctcacaataa
     9121 ccagaatcac acaatctaag tataatcctg tccatagttc taatacttac accacatata
     9181 gtagctaatt cctttgatgt aaattcagtt tttccactat ttattatttt attgaatact
     9241 ttatatacag tattagcact tagccctgtc tctgtagcaa tttgataaaa acgttcatca
     9301 aaactatttt tctctttgac agtaggattt ggctttagtg gtcccataat ttctccattt
     9361 tcaaaaacta catatgctgc atcatttttt tgactttgag ccatcttaat actctcataa
     9421 gcgttaaatt tagcttcatt tgctgttttt ccataaccaa ttcctatata agtgtttata
     9481 actgaaactt cacgaatcat atccaacaaa taaatatctc tgtatttctt tgtttctaac
     9541 tcaagtattt ttttagttgt aaaaatcata aattcacttc tactctgctc taccaaaaca
     9601 ccatctatac ggctattgaa atggtatagt ttatcaagaa tcttaatcct ttgtgaaaga
     9661 taagcgtact catcttcctt cattagtgaa tattctgaag gcatgttcag tttaatagca
     9721 agaacaacaa tctgattctc attgtttttc tttgcaatat aacgcatctg aatgtttttt
     9781 atactttgca ccattacgtc ctcagaaggc aaggctaaaa ctactggaat attatctttt
     9841 ttcattatat gataggcctc ttcaagacct gtaatacaac aacatacatc attattttga
     9901 taattatatt tatgaaattc aagcacaaac tttatatagt cctcttctaa atacttttgc
     9961 tctgcaaaaa acatatgttc atccttaaat tcaactccaa tttctttata caatctcttc
    10021 atcatagcct tatcataagt atctatacta agtctactaa tatctaattt aagcaaatat
    10081 gtaatttcaa gcaacactct gtacaatgtt gtttcatgtc taggaatata atcagacaat
    10141 acaaaatctt tttgtctttt ttcaaaaaat ttaaatggcg acttacctgt aaaaagaact
    10201 gcatccactc tacctctata tttttccaat aaatcaggta cttcaatata tctatcatat
    10261 ctaagaaaat ctgcttcaat attaggaaaa ttctttttac ttactgctag tattttatcg
    10321 attaccatac ctggtcctac ataaagtatt ttcatttcgt ctctcctttc ctaatgatac
    10381 tattatctct taaaacattt tctaaaccta ccatgactaa agccacagga ctcctgattc
    10441 atagaatttt gaataatcaa ttatacctaa tataaaaagt tactttaagt tttttaatta
    10501 cgactatctg ttgaaacatt tttgtattta aatttttgta tttaaatctt attcattaat
    10561 attaattaca ttttacaatt aatatattgt tttttctact taaagttaaa ttctttagaa
    10621 aacacaagta gagtaatctt tattaattta acaaaaatag ccttccttaa tattcaaaga
    10681 aagactattt ttataaaagt tcttaatgta ttctattttg ctatgctaag aggattttta
    10741 attacacgtc cagcacatac atcagtttgc tttcctcctg ccaatgcaat tttaccattc
    10801 accaacacat attccactcc cactgtatac tgtttaggat tactataagt cgctttatct
    10861 tctattgtat ctgggtcaaa taccacaatg tcagcaaaat aattttcttt aagtagaccc
    10921 ctttctttat aaatgcctaa gcgttttgct gaaagataag ttatcttttt aactgcttcc
    10981 tcaaaagtca acataccttt ttctctaaca tagtaagaga aaattcgtcc aaaagtacca
    11041 aatgcacgag gatgaggctt tccaagagaa agtaaacctt ctgtagaaag gtccatacta
    11101 tcagaaccta tacatgactt tggatggcaa acaaccttat ctgtatcctc ttgaccttgt
    11161 gtatgaacaa ctacttgaat tttacttttt tcttcaatca aaagcctaaa gaacgtatca
    11221 aatggatttt caccattgat tttggctacc tccattaaag ttttaccctc tacatattta
    11281 tttgcttcag tcattacaga ggctagtaca acaccgttcc acccacctgc taattggtag
    11341 aaacattgcc atccaggaat cccttcctcc aactctttta taattctgtc tctattttct
    11401 tgtacttcta atcgttttac catattttct acaccgcctt caaaagccca aggaggtaaa
    11461 agtgcaccca aagatgtcat tccagctgta tatgggtaac aatcaaaagt tacttccaaa
    11521 ccatctgcct cagcttcatc tagcttttta aatacctcat caatagttcc aaaattctta
    11581 tgacccataa cttttaaatg agaaacatgt agaggaactc ctgcagtctt agctatataa
    11641 attgcttcat caagagcttc catttcttca tcaccttcat ctctaatatg agatatatag
    11701 tatccattgt atggtatcaa ttccttacat aattcagcca tttcttcttt ctttgtgtat
    11761 gctcctggta aataaactaa tcctgatgaa agagctacac atccagcttc catagctttt
    11821 ctaacttcca acttcatatt tttaagttgc tctaaagtag ctggttcctt tgaaaatccc
    11881 atttcattga tacgtacagg tccttgtgct acataagcta caacattgac tgctggagaa
    11941 ttttcagtta agtaatcaaa gtattcttgc attgtattcc aatgaagctc taattttgtt
    12001 ggaattgtac caattatcct agttccaaga taatcaatta actcttgctt cttctcttct
    12061 cttatcggag caactcctat accacagttt gcaataacat ctgttgttac acctgtatgg
    12121 atacggcttc ttgtgtattt atcatacaca agagataaat cagaatgagt atggaaatct
    12181 ataaaaccag gtgatacaaa ttttccagtt gcgtctatag tctctttagc ctctttttga
    12241 cttaaatctc caatagccat gattttgtct cctacaatcc ctacatcact tttatatctt
    12301 gaatttcctg taccatcaac gattattcca ttgacaattt taacatcaaa catgcttttt
    12361 ctcctttcaa gacttattta tattcatatt tcttaataaa caatcctatt cttttttctc
    12421 aacaaataat tctagtcttt ctccttaata agcaatccta ttcttttgtt ttattgttat
    12481 caattacaaa gaagaccctt gtgataagta tcccacctaa cacaataaac aatgttaata
    12541 atccacctgg tataccaatt ttttcaccaa agtacataat tgctaacgct acagcaatcg
    12601 tacatccacc agctcgaata tccttacgtg caatatcaac ataaattgct ccaaataatg
    12661 ctggaagtat ataattaaat gaattagtaa caaattcagg taacaaagga attactttat
    12721 agcctacaat ggtaaaaatg gtaatcatag aagctgatac aaagattgaa caacatgtac
    12781 caattgttcc aacaacttca ccctcatgag ttcctgcttc tacacctgca attttctgtg
    12841 ccattgatgc tgcaggcata cgtatatctc caacagaacc tgcaagccaa cctatataag
    12901 aacctggagc tcctagtgta ggataataag ctataggttg aacaatccaa ctaaccccat
    12961 aagtcgcagc gactaacccc caaatttgaa atataacaga aagcattggc atctgaccaa
    13021 agcgaatacc tacatagata gcgggaataa agtttgcaat aattgcaaga cctatagaaa
    13081 ttctccctat ttttatcatc tttttatcat acataattat cacctcttat ccgttttaat
    13141 cacaataata taatctgtgt accaaacata ccaattaaca tggcaatacc taaggataat
    13201 tcttgtaatc gttgattctt tttaaataaa gttgtcaata ctagcataac tatagctgaa
    13261 ataattgctg gtgtaagttt tggtatttca aatgttttta tttgatttga aagaaggtaa
    13321 gcaaataatc caactgtagc tcctgcaagt agttttttaa cccaagtagg attatacttc
    13381 atgttcatct tttcaacaat tccacgcatt ctatgaccca aaataaatac aacaaatagc
    13441 caacctaagt tatttaaagc catcccccat agtgcatacg aaaaagcttc tgctccaaat
    13501 gcagcactac ctacttctac cccagataaa ttggatgtaa gagatattac tccaagctct
    13561 gttctagctg ctccgacatc acagagcctc atccaagtcg taggagcgcc aatcacagct
    13621 accagtgata ataatgtaat aacaggagat agagaaggtc caatggctgt aacactagct
    13681 gagcgaattg ctgctttaat tcctttcctt tcaataccaa gatttgaagc ctcttttagt
    13741 gcctctttca taaatacgat tgcttgaaca actgaaataa ttactaaaaa actagaaaca
    13801 atccaaagac ctgggctatt aatcatttct tgaaacttca tactttattc ctccttaaaa
    13861 taaatttttc agcattttat tgtgagcttt ctttctgtaa tcttgttatg tttttatatt
    13921 gttttaattt attttatcta agatatagta tctttaatag gagattatct gtcttaaagt
    13981 ctcactaaaa acattaatac ttaaatattt tattcataat tagtaaatat cagaaaattc
    14041 acatattatt tttttaacct gtaattaact tataaggtct tttattattt tgacatttca
    14101 atataaaaat tttaatcaaa aaactttatt attttaaaaa aaattattat ttttataatt
    14161 tatatttttt aagtttattt atgttcatct ctatctaaaa taatgacatt tctaactctt
    14221 aattctcatt taagtttcat tgttttttct taaaagccta cttttagtca agatactgtt
    14281 atctttactc attatttaaa gaacttattt taaatctgta catgaagtaa ataatccaaa
    14341 agcattgtta ttctaactac tcaacattaa atacttacta ttttctaatt actcaatata
    14401 aacacttact ataaaactaa atatataaat ggcttctaaa gtttaatata tctacatggc
    14461 tttatatatg atgggaaaat actagaaaaa aatatattat agaatatttg tatagaatag
    14521 gcaaaacttt caaacttaaa tttatcaaat aatcataaaa aaagtgaacc atctatagta
    14581 aaattttatc taataagtga aaggcaaaaa agtagcgcta cgatttgcat agaagggaag
    14641 gaacttagtt ggataaaact acatttacaa acaatatact tgaatcagaa cagactttgt
    14701 atcgagtatc taagtctatt ctaggaaacg accaagactg tgaagatgcc gtcaacaatg
    14761 ctattttaaa agcatatgaa aagttggatt ctcttaagga agaacaatac tttaaaacat
    14821 ggctgattag aatagtcata aatgaatgta attcattaag acgcaaacga ttaaaatctc
    14881 tatcatttga agatgtcttc aaaaacaaaa aaattgatga aaaagatgac tatagcgatt
    14941 tgtatactgc tattcaaagt ctacctaaaa aaattaaaat tccaatagtc ctttactata
    15001 ttgaaggtta ttcagtagat gaagttaagg aaatattaga tatacctcaa ggaactgtaa
    15061 aaagtagatt atcaagagga agaagattat taaaaactaa attagaaaac acggaggtaa
    15121 tcatatgaat aaaaatatat taaataattc gtttccagaa gttccagaaa gttttcataa
    15181 tagtttaagc aaaacactaa atagtttacc agaaagagag gaaaattata aaatgattaa
    15241 taataaaaca cacaaattac catttagaaa aggcttagtt gcaactttag ccattacttt
    15301 gatattgagc acaacagccc ttgcagtagg acatatatat agtttggttg gaaaatcaag
    15361 tactaaggct acctatacat ctatcccaac tacagaacaa gtaaaaaaag actttaactt
    15421 tacccctaat atagtttctg aatttagtaa tggatataaa tttaaaggag catatactgt
    15481 aaataaaaaa gcatttgaca aagaaaataa tgttttagga aaatcaaaag cacttagttt
    15541 tgaatataaa aatggcaatg atgaaatatc tcttgacaca gcaaataatg tacttgggaa
    15601 gagttctgac aatctaaaag ttgtagatac ttacaaagat attaatattt cttatgaagc
    15661 attcgcacaa aagtttgtac cagaaggtta taaaatgaca gaacaggata aaattgatga
    15721 aaaatcaggc aaatacacat ttagtgtagg gccaaataat gaatcagaca aagttgaagt
    15781 taataaattt aagtttttga catggaaaca agatgaagtt tactattctt ttacagttca
    15841 agactcaaat ttatctatga atgacttagt taaaatggct catgaagtaa tagatgcaaa
    15901 ataaacataa gtaataataa gttgtttatg agtttattat ttatgacttt aattttttta
    15961 ttagtaaaca tggttgtatc ttccatcttt atattttctt taaatttaat atataaaatt
    16021 ttagatattt agctaccttc aaaagaacca ttattaaaaa gcccttaaaa atatttcttt
    16081 tatatggaat tttaagggct ttaactttat tttatacaag tgtatatgga attttaaaga
    16141 ctttaacttt attttatata agtgtaagct ccatttgaat tacttcttct tttacaggtc
    16201 tatcattcca tgtaaaacca ctagatattg tatttacctc tacaaaccct aaagatttat
    16261 aaacatgaat agctctacta ttacttttaa atacatcaag ctcaattttt tcatacccat
    16321 attcttttga taaatcaata atttgtttta aaagattact tccgatacct ttttccctat
    16381 actctttaag aatacccatg cctaaataac caactttttc agttttaggt aaaacgtcac
    16441 accaaccaat acagttatct gattctaaat caattataaa cagttgtggt aagtttttat
    16501 ttataatatt ttttataaat tcaactgttt cttcaaaagg aaaggcttca tccatagcta
    16561 gatatttcct ctcttttgct attttatcaa aagtttgcca atacgaataa atatactttt
    16621 cttctgcctt tataaatttt atgccttcta tcaacttaac atttcccctt tttaatttgt
    16681 gattgtagtt aacttatttt atatgattct agtttataat gctatataaa ttactatata
    16741 ttagcttaaa atatctttgt atatttctat aaaattaaaa ccaatatatc tatctct
//
LOCUS       DAEOSP010000069        16678 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.069, whole genome shotgun sequence.
ACCESSION   DAEOSP010000069 DAEOSP010000000
VERSION     DAEOSP010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16678
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.069"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            148..510
                     /locus_tag="KRH54_001848"
     CDS             148..510
                     /locus_tag="KRH54_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBG8579510.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            946..1647
                     /locus_tag="KRH54_001849"
     CDS             946..1647
                     /locus_tag="KRH54_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBG8579511.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1917..2735
                     /gene="larE"
                     /locus_tag="KRH54_001850"
     CDS             1917..2735
                     /gene="larE"
                     /locus_tag="KRH54_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBG8579512.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            2790..3938
                     /locus_tag="KRH54_001851"
     CDS             2790..3938
                     /locus_tag="KRH54_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBG8579513.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            4074..5462
                     /locus_tag="KRH54_001852"
     CDS             4074..5462
                     /locus_tag="KRH54_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG8579514.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            5491..6189
                     /locus_tag="KRH54_001853"
     CDS             5491..6189
                     /locus_tag="KRH54_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8579515.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            6192..7625
                     /locus_tag="KRH54_001854"
     CDS             6192..7625
                     /locus_tag="KRH54_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8579516.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            7647..8384
                     /gene="truA"
                     /locus_tag="KRH54_001855"
     CDS             7647..8384
                     /gene="truA"
                     /locus_tag="KRH54_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBG8579517.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    8495..8702
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8955..10154
                     /locus_tag="KRH54_001856"
     CDS             8955..10154
                     /locus_tag="KRH54_001856"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBG8579518.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            10401..11447
                     /locus_tag="KRH54_001857"
     CDS             10401..11447
                     /locus_tag="KRH54_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HBG8579519.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGEYLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNNSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            11555..12718
                     /locus_tag="KRH54_001858"
     CDS             11555..12718
                     /locus_tag="KRH54_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8579520.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SRNPSSTDLQEIAKAITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(12807..13187)
                     /locus_tag="KRH54_001859"
     CDS             complement(12807..13187)
                     /locus_tag="KRH54_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBG8579521.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(13190..13849)
                     /locus_tag="KRH54_001860"
     CDS             complement(13190..13849)
                     /locus_tag="KRH54_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBG8579522.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(13955..14929)
                     /locus_tag="KRH54_001861"
     CDS             complement(13955..14929)
                     /locus_tag="KRH54_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG8579523.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(14969..15316)
                     /locus_tag="KRH54_001862"
     CDS             complement(14969..15316)
                     /locus_tag="KRH54_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579524.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(15534..16490)
                     /locus_tag="KRH54_001863"
     CDS             complement(15534..16490)
                     /locus_tag="KRH54_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBG8579525.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
BASE COUNT         6562 a         1750 c         2692 g         5674 t
ORIGIN      
        1 atttaaatat ataatgtgaa taatgtttaa taatttgaaa tatgataatt ttttatgttg
       61 aaagcttata atgattatga tataaattgg ttatagtata caatttatgg gtataacaat
      121 aaaaaattag aaaggaattt ttataaaatg attaaattat atggatatac aaaatgctct
      181 actgttaaaa aagctaaaaa ttggcttaag gaaaataatt tagaatttga agatatagat
      241 atggttcaaa accctcctag taaagaggag ttaaaatcaa tttataagac tagtggatat
      301 gatataaaga aattttttaa tacaagtggt atgaaatata gagaattggg tcttaaagat
      361 attgtaaaaa ctgaatctga tgataaactt ttagaaatat tggtaagtga tggtatgctt
      421 attaaaagac cattactttt ggatggtaaa aatgtattat tgggttttaa agaagatgta
      481 tggaaaagta ctttgctaaa agaagattag gaagagatag gtgtgttgat taaaattatt
      541 aattaataca cctataaatt tatgtattaa atctaatctt tataaatatt aattagcaaa
      601 ttcaaatcaa aaaaattcat atcaacaaac atattacttc ttattacaaa ttatatcata
      661 tgttttatat taaaatatta taatattaca acgaattgtt acaaaattac ttattattaa
      721 ccatgaacat ttgttttgtg ctatcatatt tatataaggg aattgtcata aaaaatatct
      781 tgttcataca gttttatata gaaaaaagaa atgaatcttt tctttatata agtatatttt
      841 gtaaattgcc tctatattta tagtttaaat aatagtggta ttaaaaatct gacaacaaaa
      901 ataaaacttt caaaaaatta aatctaaaaa gaataggaga taaatatgag tattgaagat
      961 ttatatgatt ttgaatgtgt tcctttgaac aatgagattg atattgatgt tttatttgaa
     1021 aagatgatag aagatggaaa agaaaaagaa tatacaccaa ttatagtcat tgatgaaaaa
     1081 gtagggctat taagaaaaaa tatagatatg attactaaag agtatggttc tcttgaaaac
     1141 tatagagagt attgtttaac aaaatataat gaaattgatg taaatcaatt ttttgatttt
     1201 aggaagagtg agatttcaga attattaaag gagctaattg atttagaaga tgatttttat
     1261 gattataaac attttagacc cattatgaat atagatatat ttcatgaata taacaaagtt
     1321 tacatagcta aaattccaac aattaaacca tatgaagtat ttgcatacat acctattggc
     1381 ggatttaatg attgtcctag agatgaagaa catatagcta ttgcaaaata ttggtatgaa
     1441 aagcatggaa tctttccaat cgcaattgga tgtgatacag ttcaatattc tgtaaaaaat
     1501 ttaactaaag atggtaaaaa atttgatgac ttatgtattg aattagtatt ttattgtgag
     1561 gatattatta tacaagggta tgaaacctta aaggctctta aggatgtatt aaaacgctct
     1621 acaatagtgc ttttttggtg ggattgatat tctatcattt agatagtatt aaattgttag
     1681 tcaccaagaa tccacttgct ttagttatag ggagtgtgtc aaactaggtt tttatacaca
     1741 gtgtagcata gtaatatgtt taaattacaa aggttaaaaa aatactttaa tatttttaat
     1801 attataatta atagatttaa ggtaattata atttataaaa taaatacttt atatgttata
     1861 atcattaaga atgtaaaaaa ataatatata gttttatagt tcatattttt ccagatatgg
     1921 aggtaaattt tgtgaaggaa aaagaaaaat tagataaatt aaaaaaaatg ttacttgaat
     1981 taggcagtgt tgtagttgct tattctggtg gagtagatag taattttttg cttaaggtcg
     2041 caaaagatac attaggtgaa aatgttatag ctgtgactat tcatgcaatg atgcattcaa
     2101 gtagggaaat tgaagaggct aaacaatata ctcaaaattt tggtgttaaa catatcatat
     2161 taaatataga aaactttgat ttaaaagaat ttaaagaaaa tggtgttgat agatgttatc
     2221 attgtaaaaa atatatattc tctaaaataa aagaggtagc aaaagaacat aatataaagt
     2281 acatagtaga tggaacaaat atagatgatt taggagacta tagacctggg cttaaggcat
     2341 tgagtgaatt aggagtaatt agtccattaa aagatagtag tttaaaaaaa gaagaaatta
     2401 ggtcgctttc aaaaacatta ggattaaata cttttaataa accatcattt gcatgtcttg
     2461 cgagtaggat accttatgga gttgaaataa cagatgaaaa tttaagaata gtagaaaaga
     2521 gtgaagaata tctatccaat ttaggttttt ctcaatttag agtgagaatg catggagata
     2581 tagctagaat agaagttgga caggaagagc taggtaaatt ttttgaaaat aacaatttta
     2641 ataaagttga tactaaatta aaaatatttg gctttaaata tgttacacta gatatgtcag
     2701 gatataaaat gggaagcatg aatttaaatg tgtagattat tttaaaaata aataagtttt
     2761 atagttaata taaggaaaca ggaggtttta tgaaaaatta tacgttaata tcgccatgtt
     2821 tttttggaat ggaaaaaatg ctggctagag agattactaa tttaggatat gaaataataa
     2881 aaacagaaga tggaagaata acatataaaa ctgatgagtt tggaatagca aagtcaaaca
     2941 tgtggttgag atgtgctgaa agagtacatc ttaaaattgc tgaatttgag gcaaaaagct
     3001 ttgatgaatt atttgaaaat acaaaaagaa taaactggtc tagatatata ccttatggag
     3061 ctcaatttcc tatatcaaaa gcttcctcta taaaatcaaa attatacagt acaccagatg
     3121 tacaagccat agtaaaaaag gctatagtag aaagcttaaa gaaaagttat ttggaggatg
     3181 gtttgcttaa agaagataag gagaaatacc ctatttttgt atttatacac aaagataaag
     3241 taaccatttc tatagataca acaggagatg ctttgcataa gagaggatat agagaaaagg
     3301 caaataaagc tccaataaga gagactttag ctgctgggct tatttattta actccctgga
     3361 aagcaggtag agttttagta gaccctatgt gtggttctgg tactatattg attgaagctg
     3421 ctatgattgg tataaatatg gcacctggct taaatagaga gtttatatca gaaaagtgga
     3481 gaactcttga taagaaaata tggtgggatg taagaaaaga tgcttttaat aagatagata
     3541 atgaatctaa atttaagatt tatggatatg atatagatga agaatctata gatatagcta
     3601 gagaaaatgc tgaaatagca ggtgtagatg aatatataga gtttaatgta ggagatgcaa
     3661 ctcaatttaa aagtgaagat gagtttggat ttataatcac taatcctcct tacggagaaa
     3721 gattagaaga taaagacagt gttaaacagt tgtataagga gcttggttat gcatttagaa
     3781 aattaaaaaa ttggtcgtat tatttgataa cttcttatga agactttgaa tatgagtttg
     3841 ggcaaaaagc agataaaaaa agaaaattgt ataatggtat gttaaaaact aactttttcc
     3901 aatatccggg tcctaagcct cctagaaata ataaataagt tttacatgat ttatttgtaa
     3961 aatataaaat tttattaaaa taagtaaaac attatgatgc cctgaaacgt ctaattattg
     4021 tgaggagatt tttgtgaaca aatttaaaga tttataggat taggaggagt taaatgaaag
     4081 tgttaaagct tattactgta gtagccctga tgatgttatt tgttactgga tgtaatataa
     4141 aaactgaatc tccagaacaa ttagcgaaga tacctgacta tgatgataca aaaaaagtat
     4201 tgtatgatgg tattgaccag ttactaaaac cagattcatc tatgatattg ccttctaata
     4261 caaaagaagt aggaaaaata aataaggtag atttaaacag cgatggaatt gatgaattag
     4321 tagtatttga acaaaaagaa gatttaagta ataatgtgag tcaggtaggg tttgtaacct
     4381 tgagttacaa tggagaaaaa tacgtacttg gagaccattt tttagaaaat ggcgaatcaa
     4441 tagaatatgc taatttttat gatttggact cagatggata taaggaagtt atcttattgg
     4501 ttaaatccaa agacaaaacg aatatgcata tatataaagt agtagataat gaaataacaa
     4561 aaatatatga cttggatgca tcatggcttc aaaataaaga agattttaat gacatgaaag
     4621 taaaaattgg atatatagat ggagatgaca agttagacat attgatgctt catttgaata
     4681 ataagactaa tgaaatgttt gcaagtgttg caaattttga tgggaaaatg aaaatcaaag
     4741 attctgttaa gtttgaaaat gtaaaaaact taagtgaatt atatataaca ttaggtaatg
     4801 ttgcttcatc tgttggaaat tcttctgcag ctataaaagg tattgtactt gatattccta
     4861 tattgaaaga taataactat attacgcaga tactttatat gaaagatggg aaaattaaca
     4921 aagcttttag tgattatgat aaaactatta ctaaatctta ttatatacct gtagatgata
     4981 ttgacaaaga taagattata gaaataccta tagttgcagg cagtacaggt aataataaaa
     5041 atacttatag ttctaaatca tctgcaacta taagttggta tagatggaat ggaaaagaag
     5101 gtgctgactc aagtttaata tttacaagtc agatatatta taattataaa tataatttta
     5161 aattatttat tccaaataat ttatttgata aaatacttgt tgaacaggaa tttgttggag
     5221 aaaaagcagt atttaaattt tactattttg actattcaga ccgtaagaat ttatttaata
     5281 tagtagtaga gagtaaaaat aaacttgaag atagaaaaaa tacaggtact caaaattcta
     5341 ttgtacttca agagagcgat gaatatactt tcttactagt tgtaaacaat gcaaatgaaa
     5401 tggataagct agatatgact atagacgcat taaaagaata tttctcatta atatatggtt
     5461 aatattatac tcaattggag gttttatttt atgaaggaaa agatacttat actagaagat
     5521 gaaattggta ttagaagttt tgttagtata aacttaaaaa gggaaggcta tgaaatagtt
     5581 gaggcaggta ctggaagaga agctatagaa aaaatgacaa cagaaaagga tattacaata
     5641 gctcttttag atgtaatgct tccagatata agtggtatag aagtatgtaa atttataagg
     5701 gagaattttg accaagtagg tataataatg cttactgcta aagctcaaga agatgataaa
     5761 atagaaggat ttatctcagg agcagatgat tacatcataa aaccatttag tataaaagag
     5821 cttttagtca gagtttctgc tcttcttaga agagtagcaa aggatgattc aagtgttaag
     5881 tcgagtgaga tagtttctcc accatttata cttgatatag acaaaagaaa attatttaaa
     5941 aatggaaagg aaattgagct tacaccaact gaattttcaa tagtgaaata tctaatatca
     6001 aatgctaagc agtcgttaag tagagaccag atattagacg aagtatgggg aactaactat
     6061 ttatatgatt ttaaaatagt agatgtaaat ataagaagga taagaaataa aatagaagat
     6121 gacccatcaa aaccaaaata tatacaaact atttggggtt atggatactg ttttagaaag
     6181 gaagaataat agtgttacat tttgaaggtc tgagaaaaaa agtaataaaa aactatttta
     6241 taataattat tataatggtt actctgtttg aaggattgtt catgttttac atacaaaact
     6301 actattatga ttctgtcaaa cagctacttg aatctgaaat aaaatatgct gatgaatata
     6361 atgctattac aatggaaact acaagttttg agaaaaaagt aaaaaatata tttgataaac
     6421 aacccttaac taaaaattca gaatttggaa tttcaataat agataaagat aaaaatatta
     6481 tattagacca atatggtttt aaaagtaaag aaaaagcaaa ctatgaagat gttaacaatg
     6541 cattaaaaga tataaaaact aaaaatttaa caccttatac ttatagaata ccagatacag
     6601 gggagcatgt tatgagtatc tccttaccac ttaaggtaaa taatataata gaaggtgttg
     6661 ttagatatac agtatcatta gatgctattg ataatgcaat attaaagcaa gctacatggc
     6721 ttatattagc aggaatattt atacttatta tagctatact aataagtctt aaatttgctg
     6781 agactcttat aaaaccatta cgagagctaa agaaatttgc aaatgaattg gctgtaggaa
     6841 attataatat aaaattagaa aagatgaaga ttgtagatga tgaaattggt gatttagcac
     6901 agacatttga acacatggca catgagattg acaaaagtga aaaattaaaa gaggaattta
     6961 tatcatctgt atctcatgaa ctaaggactc ctcttacatc tataaagggg tggagtgaaa
     7021 ccttaggtta tgaaagtata acaagagaag aactagactt aggtttaggc attatacaag
     7081 atgaaacaga aagacttata aaactagttg aagaattatt agacttttca aggctttctt
     7141 cagacaggat aaaacttcat gttgatatag ttgatgttga aggtttaatt gtaggtgttg
     7201 taaatcaact taaagttaaa gcagctgaaa aagatatatc acttttattt gaatttgaaa
     7261 atgaatttat agagaacatt caaggagata aaaatagatt aagacaggtt ctcataaatt
     7321 taatacaaaa ttcatttaaa tttacgagtc aaggtggtta cataaaagtt gtagcttctc
     7381 aagatgaaga gataacaacc atatcagtag aggataatgg ttcaggtata gaaaagcaaa
     7441 atttaaataa agtgttagat aaatttttcc aagaagatta taataaggct ggtagtggat
     7501 taggactagc aattagtaat gaaattgtaa aacttcatgg tggaagaatg aaaatagaaa
     7561 gtgaaaagaa tgttggaaca aaaataactt ttaatataaa aaataaattt gctaaacaag
     7621 cataaattct tagaaagagg attaagatga gaaacataaa aatgatagta gcatacgatg
     7681 gttcaagata taaaggatat caaaaattag gtgacaataa tatgactatt caagaaaaac
     7741 ttgaaaatgt tttaagtaaa atgactaatg aaacagtgga gataataggt tctggaagaa
     7801 ctgatatggg agctcatgca agaggacaag tggtaaattt tagaactaat tgtatggatt
     7861 ctttggataa aattcaaaaa tatttatatg aatatttacc agaagatata gttgtaaaaa
     7921 ctgttgaaga agttgatgaa agatttcatt caagatacaa tgtaaaatca aagacttata
     7981 tgtataaaat tgacaataat aagtatcata atccttttat aagaaaatat gctactcatg
     8041 taagtaaaaa attagattta gatagaatga gaaaagcaag tgagtattta gtaggagaac
     8101 atgattttac tagttttgct tcttctaaat ctaagaaaaa atcaaatgta agagaaattt
     8161 attctataaa tataaaagaa gatgataatg ttatagaaat atatgtagaa ggaaatggat
     8221 ttttgtataa catggtaagg ataatagtag gtgctttgat agatgttggt ctaaaaagaa
     8281 gagctccaca agatattaag tatatgcttg aatcaaaaga tagatgtcaa tcatcagata
     8341 ctgcaccagc taaaggacta tgtctttgga aagtaacata ttaatcaatt atataaaact
     8401 ctctttaaaa taaaaataaa ataaattata tagttgacat aaatggaaaa ataaactatt
     8461 atatatttta taagagagat ataaaacttt atatttaaat cctgtgagaa agagagtaac
     8521 ttctatgtgt tttatagaga gctgaggatg gtggaagctt ggcaaataag tattagtgaa
     8581 aagaactttg gaggatacta attgaattta gtatgttagt aggtaatctc gccttaagag
     8641 aaagagtgga taagtttatc ttatcaatta gagtggtaac gcggatatat ttcgtctctt
     8701 ggtttttcca agaggcgttt tttaatatat ttaattatta gtgtttatct ggtatgttga
     8761 acatttacta attgattagc taagtttatc aataagagtg gtaccgcgga tatttcgcct
     8821 cttagtattt taaaatacta aggggttttt ttagtattaa aatgatttta tgtgagtcta
     8881 aattctcaga gtcattcaat attaagaatt tctataaaat aaaaattata atttaagatt
     8941 gggaggaatt aaaaatgaag gaaaaagtag tattagcata ttcaggagga cttgacacat
     9001 caatcataat accttggtta aaggaaaatt atgaagatat agatgttata gctgtttgtg
     9061 gaaatgtagg tcaggaagat aaaatggaag atgtatatga aaaagcttta caaagtggag
     9121 catcaaaggc atatgtggat gatataagtg aagaatttgt aactgaaact atatttaaag
     9181 ccgtaaaagc agaggctaaa tatgaaggaa aatacttatt aggaacttct ttagcaagac
     9241 ctataatagc taaaaaatta gttgaagttg ctcataaaga aggtgcaaaa tatatatgcc
     9301 atggatgtac aggaaaaggt aatgaccaag ttagatttga agctacaatt gcagctcttg
     9361 acccaactat aaaagttatt gcaccttgga gaatttggga tataaaatca agagaagatg
     9421 ctatagacta tgctgaaaaa cataatataa aggtaacagc aactaaagct aaaatatatt
     9481 cagtagatgc caacctttgg catgtttcaa cagaaggtgg agatatagag cacctagaaa
     9541 atgaacataa aaaagatgtt tataaacaat gtgtagaccc agaagatgca tgtgatgtag
     9601 ctgaatatgt agaagtttac tttgaaaaag gtgtacctaa aaaggtaaat ggcgaagaat
     9661 tatctccagt tgcactgatt cataagttaa atgagttggg ttgtaaacat ggaattggtg
     9721 ttatagatat agttgagaat agacttgttg gtatgaaatc aagaggtata tatgagactc
     9781 caggaggaac aatcctttat gaagcacata atattttaga aagtgctact ttagacaaag
     9841 atacattaca tttcaaacaa atggtatctt ataaatacgg agaacttatt tacaatggac
     9901 tatggtattg taaattaaga gaatctatag atgcatttat ggaacaaact caagataatg
     9961 ttacaggaac agtaaaagtt aaactttaca aaggaaatat aaaacctgct ggaatattta
    10021 ctgaaaatgc tctttatgat gaaggaatat cttcttttgg aaatagtgaa ctttatgacc
    10081 acaaagatgc agaaggattt attaatttat tcactttacc attaaaaatc agagctatga
    10141 aagctggaaa ataataaaat aaattattta agagtgtata aaaacgattt ttaggttttt
    10201 atatgctctt tttttatttg aatttattaa attatacagt aaatagtata aaaaaatata
    10261 ttatagttcc aaatggctta ataatgtgaa aatataagac ctaaaatata aattttataa
    10321 atacaatagt tattgactct ctcattgtaa gagggtttat actgaatcta atgaaaattt
    10381 atagtgagga gatagattat atgagaaaaa taattgaaat taaacatcat tcacatgatt
    10441 atgagtgtat gtggaatggt atagaagatt tatatataaa taaaaccggt gaataccttc
    10501 caaacgattt tttcttttta ctgtcaggtt ttggttcatt ttgttatatg aaaacaaata
    10561 aagctgattt gaaaagaatg gtagctttgg gagatggaag gacaaaacag atgtataaat
    10621 ttttagcacc aattgttgga tttgaataca aacatcatga gtttaaaaag tatgagcaag
    10681 cattaaagaa agcaaaatct gaaattgatg atggatatcc agttatttta ggagctttag
    10741 atatgtatta tctaccatat tataagaagc tatatcataa agaacatatt ccttttcatt
    10801 atatattaat ggtaggatat gatgatgaag gaatatatct gtatgattgt ggtagaacag
    10861 aattattaca tctttcttat gataaattac gtgaaagcat gaattgcaat tcccctggat
    10921 taagcaaaga aaatactatt tgcacaataa gaatgaatac ttcaaagaat aaatataaca
    10981 ttgctagaga agctattgct ataaagcgtg atatgttttt aaatccacct aaaggatttt
    11041 tgggatataa aggttttgaa aagtttatta aagagttgcc acagtggaac actgaattgg
    11101 ataaggatga ttatgataag attttgacca atatggttat gttttttgga acagtaccaa
    11161 caattccaaa tgcaatcaaa ggaattaaag aaccagatac tgttcaattt aaaggtggat
    11221 ttgataaaat gagtagaatg ctcaacagta ttggggagga atacaaaaac aactctttta
    11281 tacaagtagc tgatattttt gataaaactg ctattataat tgagaaaata tccaatatta
    11341 taatagatta cttaactcaa acatgtgatg atacagaaca acttccgtta ttgttctctg
    11401 aggtcttaga gcatatgaaa acaggatatt taatcttgga tgtataaaaa atatattgtt
    11461 gaccctaaca ttacgtaata gtgtaatatg aaaatatagc ttaaatatat tagtaggtat
    11521 tgatggctgt aaaattaaaa tttgaggtgt ggatatgaga acagtgaaac aagtttctga
    11581 tttaactgga ataagtgtga gggcattaca ttattatgat gaaataggtc ttttaaaacc
    11641 aaataaaatt acagatgcag gatatagact ttatgatgat gaatctatta aaactcttca
    11701 acaaatttta ttttttaagg agattgatat tcctttaaga gaggttaagg aaataatgtc
    11761 aagccaatat tttgataagg ttgaagctct aaaaaatcaa aaaaaattac ttattttaaa
    11821 acgaaagaga ctggatgaat tgatagagct tataaatcaa actctaagag gagaaggaaa
    11881 tattgatttt aaagagtttg atatgagcga gtattttaat gttttagaag aatttaaaag
    11941 agagcatagg aataaggtaa ttaaaattta tggtagtgta gaaaaataca atgaatatat
    12001 agaaagagtt aagtctaatg aagagaaaat tgctaaaatg gctataaaac aatatggaac
    12061 tattgagaag tttgctaaag ctataaaaac aaattttagc agtgatatat tgaatttagg
    12121 agaaaaattt gatagatata aaaatgattg tttaaaagaa aaacatccaa aattaaaaga
    12181 attatataga aagcttgtag aagatttaag taggaatcct tcttcaacag accttcaaga
    12241 aatagcaaaa gcgataacag atatatctaa aaaagattat gaaattttct ctatggatac
    12301 tggtgatgac aactggtatt atatggttca aaattatcta gtaaatccta tgtggataga
    12361 agaagtagat aaaaagtatg gaagtggagc aggaaagttt attggacaag tactaaagac
    12421 ttatctaaga gatagaaaac caaagataaa tacattgtat gaaaaacttg tagaagattt
    12481 gagtagagat tgttcttcaa gggaagttca aagcattgtt gaagaaatag acaatgaaat
    12541 gaaaagaagt aatgaatttt ataaaataga caatggtgaa agatactttg attatatgtc
    12601 agagctttat ttacaagatt ctaactatat aaaagtaact gataagaatt atggtgatgg
    12661 tgcatctaag tttattggag aagcttttaa gatttatttt gataataata attgttgaaa
    12721 atttggaagt aaaaaatatg taagttttat aaaagcacaa aaataaggat gtagattagc
    12781 tccatctata tccttattta aagttattaa aatatatagt ttacatcaaa cttaacactt
    12841 tcaataatat ttcccaaata tgtttttata ctttcaactt cttctaaaga ttttaattta
    12901 ttttcagtat aaattgcttt gtccagaata ttaaacctta atcctatttc atcaaagtac
    12961 tcatgattta aaactttgta ccaatggttt ttttctttat tccaactcgt agatattgaa
    13021 tcagcttctt tttgagcttt ttcccaatct tcattttcta tagatttttc taaaacagat
    13081 atattgtctt tatatccttc tgtaaaatca tatagtttat tgttaacgta aaatccaaat
    13141 aatacaaata agatagtcca tagtataaca aatgttgtag atttcaatat taatcaccct
    13201 ttttattttg aataaatatt ttgtcatttt catctaaaac acaaataaga acatctttaa
    13261 aagtttctat gtgattagat tttaaaatac ccattaacca gttagtatct ttttgagcta
    13321 tgtctagatt tttatttatt attctaccat ccagtataag tgggataggt aaatgattaa
    13381 aagctcttgg aggagtacta ttgtagcttg atgctggtac aacacttaga ttaccatcag
    13441 tctccaaaat agcatattgt acatctttta ggttaaagta tccttgaatc ctaagttgtt
    13501 ctaataattc atctatacta attctttctt ttttaagctc tttatataca atttttcctt
    13561 tatctattaa tacagaaggt ttacctgata aaaatgaact taattttctg ctttttaatg
    13621 ataagaatga aattagagtc tgcatgatta caagtccaga tattgctgca acaccattta
    13681 tgattggtat attattattt tccattggga cagatgcaac ttctgcaatt aatagtgtta
    13741 taactaaatc aaaacagttc atttcagcta tttcaccttt tcccatgact ctaagtgcaa
    13801 ttagcactgt tatgtataat ataatacttc ttattaatac tactgtcata tctatcaatt
    13861 actcctttaa aaaatattta cacttagtta gtatatccta tataaaaaat ttaattcaaa
    13921 gtataaaaaa ccttaaataa ttggattcaa ctatttaagg tttttttgat aattttaata
    13981 ttaaattttt tatggatttt ctgttacttt tatttatttt aataacagat tcaacaaaac
    14041 tctttttatc tcctaatttt aagataaaat caggatattc ttcaaattca ttgagtttta
    14101 ttaatttgaa agaagaagat agatttttaa agttaataat tttagagtta ttcactaata
    14161 cacttctttt tttaaaataa atttcttttt tacttatatt tagtatagtt aatatttgtt
    14221 taagatatat tattatatct tctggagttc tatcaaattt gagttcaaaa ttttcatctt
    14281 caaagtaggg aatataatat aaaggtgttt tgagagcagg taatttatta tataattctt
    14341 caagaatatc gatattttta ggggagttaa agaaaaagca tttacataca ttcattaaat
    14401 ccacagcatc tgaaagtgag ttatgtttaa tatctccttc tacagaaaaa atttttttca
    14461 tattcagtaa agatatttgc ttagataaga ttctattatt gtatgtaata tgttgagata
    14521 taaaaggttg aatgtcaatc atcatattaa ataactctaa gcatttacct gtatatttat
    14581 acactctaaa tgaagttaga aggccagtta tatcaaaatc accccaatta tatattgtaa
    14641 catcatcaaa tttacaaaac catttataaa aatcatccat aacatataaa agattaggag
    14701 cattattgat atctagttgg ttcaatttgg ttaaattttt acatttattt gaaagtattt
    14761 tattgtattt tggcttaact aaagagtaat aattatctat tgtattaaat tcgttatcaa
    14821 ctaaaactcc accaatagaa attaattccg caccattata ttcttttggt ttatttttat
    14881 tatcatctag tatattaaat tcaaagtcta taaataactt gtatttcata aatctaaagc
    14941 acatcctcag gtaaattatt tattgagcct aagcatcata agaatcaata gatataatga
    15001 ctctaaacat caaaggttca ttacaacttt tatttatttc ataatttaat tcaaataaat
    15061 cttttaagat ataatttata agtattcttt cccaacccat ttttaaatta actttagagt
    15121 ctattaatat attttctatc tttttaaata tttctatatt tgctggtatt atactgagct
    15181 caatattact tttaaacggg ttagactgta aatctgttat aagtatattt tttaattcct
    15241 ctgtaagttc agaaattaaa ctatcaccta tgtatttttt tatgatttcc tctctaattt
    15301 cttttaaatt tagcaatatt agtacctgct acttacatag tagcttttcc tttcctttta
    15361 atactattat tcatttttga atcttattta gatatatttt aagattaaaa gtctttagct
    15421 atataatttt ttacccattt ttaacttata ttaacatatt acaggcttat atctccaccc
    15481 tgtttacatt gaggatggag tatattgcct attttgataa aaatgatagg ttgctatttg
    15541 cctattcctt tttctgataa tttttctaac atatctttgg tcattttatc taaatcataa
    15601 ctaaacttaa agttccattc ttttactgca ctactactgt ctagagagtt aggccaagaa
    15661 tcagctatac tttgtcttac agggtctaca tcatattcta tcacaaaatc aggaatatat
    15721 ttttttatag aatcagctat ttcttcagga gaaaagctca tagcagtaat attaaaagca
    15781 tttctatgga tgagtttatc agctggagct tcgattaaat ccactattga ttgaagtgca
    15841 tcaggcatat acatcatatc catttttgta ccttctgcta tgtaagattt atatctctta
    15901 ttttttaatg cctcataata tatatcaact gcataatctg tagttcctcc accaggaggc
    15961 gtaacataag aaataagacc tggaaatcta acacctctag tgtctactcc aaattttgag
    16021 tgataataat cacatagtaa ttctccagaa actttagtca caccatacat agtattaggt
    16081 ctttgtaatg tatcttgagg agtcatattt tttggagaat tttcaccaaa agcagcaatt
    16141 gaactaggtg taaaaaattt acaatccaat tctttggcta tttcaagacc atttattaat
    16201 ccattcatat ttaagttcca agcttctaga ggtttacttt cagcaactgc agaaagtaaa
    16261 gatgctagat gaattatagt atctacatca tatttttttg caatttcaaa aaaacgattc
    16321 ttatctgtaa catctaatat ctcaaatata ccagattcac atactggatt accttctttt
    16381 actctccttg aactagcgat tacattttgt tctccatact catttctaag ctttattgtt
    16441 aattctgaac ctatttgtcc taatgcacct gttataagta tttttttcaa ttttacaacc
    16501 tcctaagggt ttaatttatg tagtactact tatttttatt ttagcaaatc ttttgtttaa
    16561 atacaaagaa ttaaagataa taatatacga cttaattgaa tgaagtgcag taagtgcgta
    16621 aaaatttaaa gctaatatat aatggtctat tgtataacaa taaaagagta gattaaat
//
LOCUS       DAEOSP010000070        16671 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.070, whole genome shotgun sequence.
ACCESSION   DAEOSP010000070 DAEOSP010000000
VERSION     DAEOSP010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16671
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.070"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            152..2221
                     /locus_tag="KRH54_001864"
                     /pseudo
     CDS             152..2221
                     /locus_tag="KRH54_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            2218..4083
                     /locus_tag="KRH54_001865"
     CDS             2218..4083
                     /locus_tag="KRH54_001865"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024642.1,HMM:NF024754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBG8579526.1"
                     /translation="MKRNWVSKEDWSPAAGFRLEVEAMNTVINDKNTLVVAGPGAGKT
                     ELLAQRACFLLETNTCRYPKKILAISFKKDAAENLKERLELRCGKELASRFESKTYDA
                     FAKEIVDRFRNSLDEAYRPLNNYNIAENRDVRRAFEIAGLNFRGNQTEFNKVYGNKLS
                     INKLPLHTDFVDKIFIDAWNVLLKGNKDTNFVSSLTFGMISRLAEYLIRTNRYIKKAI
                     EMTYSHVFLDEFQDTKSIQYDLVKACFFKTETIITAVGDGKQRIMLWAGARKSIFEDF
                     QNDFLSQKRTLIMNHRSAPRLVEIQKAMYNSLNEEAIDLKTNEKWNYEDGEVYLRLFE
                     DYIKEADILSEEIVELVETGIEINEICILVKQSVDIYSKEIINKLSERNIKARNEAMY
                     QEILKEDIIKTLVSLFRLSLSDRNPDDWDYIYDILGELYGIDEGYNPKILNDFITKVE
                     LMINDLKVNLNITDEKQFSDLVDSKVEYISYEKFASKFQKYKSKDYFDDLVDKFKKLV
                     WIEYNETKDWIKAIDNFEGKNSIPIMTIHKSKGLEYDTIFFVGLEDSAFWNFKNQPDE
                     DRCAFFVALSRAKRRIDFTFSLDRPAGFSNSQKHYCINEFYELFDNTEIVKKIEY"
     gene            4207..5021
                     /locus_tag="KRH54_001866"
                     /pseudo
     CDS             4207..5021
                     /locus_tag="KRH54_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5046..5534
                     /locus_tag="KRH54_001867"
     CDS             5046..5534
                     /locus_tag="KRH54_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBG8579527.1"
                     /translation="MYEKDYKQYIKLDIPIDEAKIKNRFEIRLKNERAYHAAVDLLAN
                     RDEEKTAFSIINRYIRFVDKDDEKIRSEWKTNDQWSYFIGEGRAKLKLTTNPEPYNIQ
                     KTLNWLSRQVAPTLKTIKRLDSLNNSTLIEGLIRYARLTDKHKKIIEQNSVNVEDIIY
                     DE"
     gene            5647..5808
                     /locus_tag="KRH54_001868"
     CDS             5647..5808
                     /locus_tag="KRH54_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579528.1"
                     /translation="MAIAIIGVYLGFKREFSKLIGFLVVALIAVGLVFNTAGVKDILL
                     ELFNKIIGV"
     gene            6048..6275
                     /locus_tag="KRH54_001869"
     CDS             6048..6275
                     /locus_tag="KRH54_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8579529.1"
                     /translation="MDIVKVFANNVRKYRNKKGLSQEAFAEKAGLHRTYICAIERKKR
                     SIALDNVQKIADALEIETYLLFIEGEEKECL"
     gene            6272..9211
                     /locus_tag="KRH54_001870"
     CDS             6272..9211
                     /locus_tag="KRH54_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBG8579530.1"
                     /translation="MKKLPIEKLIENLGFSGSKNLVNIDMLTKLDISKHDVKTLKVIN
                     PYAIYLVNSKPFILFIESSFEEKKLQQISKLIWNAQIPIAFICDSNSVKIFNGKALDL
                     KSYLIEEIYNQSIEDFSEESEFSYLKISDPLFWDQYTKQYTKTHLNDYLLDNITSLTN
                     KLKKKYKVKFATKLVLRLIFIRYLIDRGVDIAYKNFNHDIKASQKELLKITKNKVELY
                     ELFKYLKEKFNGNLFELNDEINNTCLTDEVFILLSDFLSGKISIDNGQMSLFSMYDFN
                     IIPVELISNIYEILLGKEIRNKDNAFYTPNYLAEYILDKTTLEFLKNNRQYKILDPSC
                     GSGVFLVNSFRRIIDMNLGDKLFCEDDKLLKELLLSNIYGIDINEDAIDVTIFSLYLT
                     ILDYKDPKILSEFKLPNLKGCNLIASDFFDDNKLESLKKINFDFIIGNPPWGSVSDGL
                     HLDYCNKNGYNDMQQNKEISRSFIFRAKDFSSRNTVCSFVVHSKILYTQKKPSKKFRE
                     FLLKKSQIFSVVEMSSVRKLVFKNADAPAAVISFKYNESEENLNNRIIYTSLKPNVFF
                     KLFNIIVIEKYDVKYISQKLLLENDWAWKTIVYGFSKDIDIIMNIKKKYQTVNEIILK
                     NSFVHGTGIQINGNESHDATHLKGKWYVDSKKGIDSFYVDINKGKELTQDKMRRPKAN
                     QQEIFIPPYVLLKKGVDTKTYRYRAAFSNDAFVYSDAVTGICGKKDNLTTLYCLTGML
                     NSSFYSYLNIMLGTSIGIEREQGFSTEIFTYPAIESDEIANLVVKIQEATKLEKTTMF
                     LNEKNSCKYIKQLDKLILKSFNLNDDKFIDYAINIQIPELTNSKSTNIYRKITKEDLI
                     NYSECFKEQFSSIYRRVGKSISISVYPKILNRFTVFELEVTDEKTDREIMVKAEADNN
                     MELFTKIIRYDYNDVFHQIRDIIHFGENSFYIIKPNCYKYWHPAIAEMDLADVLDQIL
                     SDTGGEE"
     gene            9211..9885
                     /locus_tag="KRH54_001871"
     CDS             9211..9885
                     /locus_tag="KRH54_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579531.1"
                     /translation="MGGFKNSSLNFYLKQGLYEIIIDGLIDCCLLMSKSCIEQSRKIL
                     NHEEKIRTHLVENYLENDIERRKTSLSKQHLRFEVEAQENYDNKNESYEGRVDIKVIS
                     KDTLADRRAYYIIECKRIDGKETLNKKYITEGVQRFITEKYSSHYGKNIMLGFIVKDI
                     NIQSNIQKINSLNQNILYTYIDTDMLTVRSDSENNYAVCESKYKVKSKLLLLNHIFYN
                     FSSIIR"
     gene            10046..10462
                     /locus_tag="KRH54_001872"
                     /pseudo
     CDS             10046..10462
                     /locus_tag="KRH54_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455547.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            10422..12869
                     /locus_tag="KRH54_001873"
                     /pseudo
     CDS             10422..12869
                     /locus_tag="KRH54_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            13009..13431
                     /locus_tag="KRH54_001874"
     CDS             13009..13431
                     /locus_tag="KRH54_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579532.1"
                     /translation="MRLEDIIDKESYEEKYDDLTVEIKKLSKEKKTLKASLINEKSIK
                     ARLREFRKILERNELLEKFDRYVFASIVDKVIVGVINEDGIVDPYKITFVYKTSFDDK
                     QDGHKFINKSPLETYSKDNNDNKKKCSYTNNETLRDCS"
     gene            13969..15003
                     /locus_tag="KRH54_001875"
     CDS             13969..15003
                     /locus_tag="KRH54_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBG8579533.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDERQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            15090..15293
                     /locus_tag="KRH54_001876"
     CDS             15090..15293
                     /locus_tag="KRH54_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBG8579534.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            15464..16624
                     /locus_tag="KRH54_001877"
     CDS             15464..16624
                     /locus_tag="KRH54_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HBG8579535.1"
                     /translation="MKLSKILLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
BASE COUNT         6813 a         1617 c         2917 g         5324 t
ORIGIN      
        1 aaattaaata tatttttaaa atatttacaa tagagttatt ttttattcgt ataataatag
       61 aaatatttta gcataagatg tataataaag gaaaaaatac agaattaaaa aacataagat
      121 agataagtgg acttagggga gagaaattat tatgcaattg acaaaaataa ttcttagtaa
      181 ctataggtgt tttggagaag aacctacaac tattgaaatt aataatttaa ctggatttat
      241 aggtcataat agcagtggta aaactgcttt attaagtgca cttatgaaac tttttggaga
      301 aaagtctagt gatagaatta ttgaaagatc agatttccat atacctgaaa gtattgatcc
      361 agatagtatt tctaagaatg aattatatat tgaagcacaa tttgagtttc ctgaattgga
      421 gagaggtaaa gacagcatat ctataccagt attttttaaa aatatggttg ttagtgaacc
      481 aggtgggaaa ccttatttaa gaattagact tgaagcaaat tgggagaaaa gctcaaatcc
      541 tgaaggaagt attgattcaa atgtatacta tataacatct acaacagagg agataaatcc
      601 tgaagataag cgtaatatca aaagatatga gcttgatcaa ataaagatga tttatgtacc
      661 tgccattagg agaccagatg aacagttaaa aaatgtctca ggaacaataa tttcaagatt
      721 attaaatggt ataagttggt cagaacaaac aaaggcaaga attaaaacga aagcaagtga
      781 aacggaggaa attttctttg aagaaaaagg ggttgaatca ttaaagaaat cactcgaaac
      841 tcaatggaat agttatcata aggatattag atattctaat ccaaagataa acttcaatag
      901 tactgattta gagacaattt taaaaaaagt tgaagtaact ttttcaccta cagggacaca
      961 tcgagattat cgggttgaag agcttggaga tgggttacgt tctttatttt atctttcact
     1021 tgtaaataca tttttagaaa ttgaagaaga aattctaaaa tctattaatg atggtaaaga
     1081 agaagatata tttacattaa taccgcctgt attaacaatt gttgcggttg aagaaccaga
     1141 aaatcatatt tcacctcatc ttttaggaag ggtagttgaa aaattaaatt ttatttctac
     1201 aaaagaaaat tcatagacaa tattgacatc acattcacca gcaatagtaa aaagaattga
     1261 accaacagac ataaggtatt ttaggatttg taaggaatat gaatgtacaa aagtgaagtc
     1321 aattatatta ccaaatgaag aaaaggaagc agaacaattt aaatttgtaa aggaatcagt
     1381 attagcatat cctgaacttt attttgcaaa acttgttgta ttgggcgaag gtgatagtga
     1441 agagatcatt attaagcaga tgattgaatt aaaagcagaa tctgtagatt catcagaaat
     1501 ttcaatagta ccattaggtg gtaggcatgt taatcatttt tggcgtttat taaatgattt
     1561 agaaattcca tttattactt tgttagattt agatagagaa cgagatggag gtggttgggg
     1621 acgtataaag tatgcaatac aacaattaat aaatattggt catgataaga atgtattact
     1681 ttatacatgt gaaggaattt cacaagataa tgaatttaaa gagatgcata cttgggatgt
     1741 aactgatact gaaaatatgg ctaaatggat tgaaatgcta aaaggttatg atgtttattt
     1801 ttcagctccg ttagatatag attttgcaat gttacaaagt tttaaagata aatatatttc
     1861 agcattaaca agtgcggaag gtcctaggat aaaagatcat ggaaaaatac aagatattgt
     1921 gatcaatgaa gatgctgaag atgaatcatt gaaagctgct tataaagata gaatagatag
     1981 tgatattaaa tcaaccttaa aacaagaagg tggtgatgga tctacttata ctgagggaga
     2041 gaaagaacta atgatatggt attcatattt ctttttagga agaggtaaac caactactca
     2101 tttatcttta ttttctaata atactgatat agattttgaa agttttccat actgtctaaa
     2161 ggagtttgta aatagtatta ttgaaaagat gatagatgat tcagggggtg gaagaaaatg
     2221 aagaggaatt gggttagtaa agaagattgg agtccagcgg ctggattcag actagaagtt
     2281 gaagcgatga atacagttat taatgataag aatacacttg ttgtagctgg tcctggcgct
     2341 ggaaaaacag agttattagc tcagagagcc tgctttttac tagagactaa tacctgtaga
     2401 tatcctaaaa aaatccttgc tattagtttt aaaaaggatg ctgctgaaaa tttaaaagag
     2461 cgtttagaat tgagatgtgg gaaagaatta gcttcaagat ttgaatcaaa gacctatgat
     2521 gcttttgcaa aagaaatcgt agatagattt agaaatagtt tagatgaagc atatagacct
     2581 ttaaataatt acaatatagc tgaaaatcgt gatgttagaa gagcatttga gatagcagga
     2641 ttaaatttca gaggaaatca aactgaattt aataaagtat atggaaataa actatcgata
     2701 aataaattgc ctttacatac agattttgta gataagattt ttatagatgc ttggaatgta
     2761 ctacttaaag gcaacaaaga tactaatttt gtaagttcac ttacttttgg tatgatatca
     2821 cgtttagccg aatatttgat aagaaccaat aggtatatta agaaagcaat tgaaatgact
     2881 tatagtcatg tgtttttaga tgagtttcaa gatactaaat ctattcaata tgatcttgtt
     2941 aaagcttgtt tttttaaaac agaaacaata attactgctg taggggatgg caaacaacga
     3001 attatgttat gggctggtgc tagaaagtca atatttgaag attttcaaaa tgatttttta
     3061 agtcaaaaaa gaacattaat tatgaatcat cgttcagcgc caagacttgt agagattcaa
     3121 aaagcaatgt ataacagttt gaatgaagag gcaattgatt taaaaactaa tgaaaagtgg
     3181 aattatgaag atggagaagt gtatttaaga ctttttgagg attatataaa agaagctgat
     3241 atacttagtg aggaaatagt cgaattggtg gaaacaggta tagaaattaa tgaaatatgt
     3301 atattagtaa aacagagcgt agatatttat tcaaaagaga ttataaataa actttctgaa
     3361 cgtaatatta aggcgcgtaa tgaagcaatg tatcaggaga tattaaaaga agatataata
     3421 aagacactgg tttcgctttt taggttgtcg ttaagtgata gaaatcccga tgattgggat
     3481 tatatttacg atattctagg tgaattatat ggaatagacg aagggtataa ccctaaaata
     3541 ttaaatgatt tcattaccaa agtagaatta atgattaatg atctcaaggt aaatttaaat
     3601 attactgatg aaaaacaatt ttcagattta gttgattcta aggttgaata tatttcatat
     3661 gaaaaatttg catcaaagtt tcaaaaatat aaatctaaag attattttga tgatttggta
     3721 gataaattta aaaagctggt atggattgaa tataatgaaa ctaaagattg gattaaagcg
     3781 attgataact ttgaaggtaa aaattcaatt ccaataatga caattcataa aagtaaagga
     3841 cttgaatatg atactatctt ttttgttggg ttagaagatt cagcattttg gaattttaaa
     3901 aatcaaccag atgaagatag atgtgcattt tttgttgcat tatcaagagc taaaagaaga
     3961 attgatttta catttagctt agatagacca gcaggatttt cgaattcaca gaagcattat
     4021 tgtattaatg aattctacga attgtttgat aatactgaga ttgtaaaaaa gatagagtat
     4081 tgattaagat gataaaaact ttagtatatt atattttaag atattagtta tataagtaga
     4141 cattgaattt atgtatttga tgtctatttt ttatataaaa atatattaaa gaaaggatga
     4201 atttgcatga agaaaaaaac aaaaatatct aaaaagattg taagttttat tatggttttt
     4261 ataactatag ttactactat acttacatca tcagtttcag cttatgctag cgagttggat
     4321 atgagtgaac agacaggctt taattattta ggtattagtc caattacagg aaaaaaatta
     4381 atcataacat ttatcgtatg aagatggata ataaaattgt attttgtgta gagccaggaa
     4441 ttaaaacttc aagtggagga ggatatatat cggaggagtt aggagataaa tttatatcta
     4501 caacacttcc aaattatcat aaaagaaaag ctgaaatact tgagaaggta aatagacata
     4561 atatattacc ttcatggaat aatgaaaaag ttgcagttaa aagtggagaa acaattgagt
     4621 tagaagataa gaatggtatt attagagaaa tgcaattaga atcaaataat actggaacag
     4681 atatattact tgaagaaaat actttgaaaa ttacagcaaa taaaaattct aattcaggag
     4741 ttattagtta tataaagatt cctaaaaatg aggttggaat atcaatagtt tatcgtaagc
     4801 ataactatca aagcttatca gaatttcata tggaaagttc gatgagtgca aatttaaata
     4861 taaatgtaat caagttagga attgttaaag taaaaaagat tgatgaaggc acaggaaagg
     4921 aattagcaaa tgcaaaactt agatttgagt atgatggtga tagtaaagaa attataacgg
     4981 gttctaatga aattgctagt atagaagata ttattggttg attaaaaagt gaagtatact
     5041 tttgtatcta tgaaaaagac tataagcaat atataaaatt agatatacct attgatgaag
     5101 caaaaataaa aaatagattt gaaataaggt taaaaaatga aagagcatat catgcagcag
     5161 ttgatttgtt agcaaataga gatgaagaaa agacagcatt ttcaattatt aatcgttata
     5221 tacgttttgt tgataaagat gatgaaaaaa ttagaagtga atggaaaact aatgatcagt
     5281 ggagttattt tattggtgaa ggtagagcga agttaaaact aacaacaaat ccagaaccat
     5341 ataatattca aaaaacttta aattggttat caagacaagt tgcaccaacg ttaaaaacaa
     5401 ttaaaaggtt agatagtttg aacaactcaa cattaattga gggcttaata aggtatgcaa
     5461 gactaacaga taaacataaa aaaataattg aacaaaattc agtaaatgtt gaagacatta
     5521 tttatgatga ataaaaaaat gtaattagta ttgcagattt taatataaaa gaagggagat
     5581 atacgaatga attttggtca aaatttatat aattagtttt tatcaaatgc acaatcattg
     5641 gtattaatgg ctatagctat aattggtgta tatttaggat ttaagagaga gttttcaaaa
     5701 cttataggat ttttagtagt tgcattaatt gcagtagggc ttgtgtttaa tacagcagga
     5761 gttaaagata tattattgga gctatttaat aaaataatag gtgtgtaagg attaaaagag
     5821 aatagaaaat tatgagtata tgatgaaggg ttctaataag ttttattaaa atatatattg
     5881 aaagacaagt agagctattt cgagttatta catgtaatgc taatatatat aactgcattt
     5941 tggaaattaa atacataatt agaaatagca aattttatag gtgacttatt aatatgttta
     6001 cttattgtat actatttgat atatttaata tgaggtgatt cttgaaaatg gacatagtga
     6061 aagtttttgc taacaatgtg agaaaatata gaaataaaaa aggtctatca caagaagctt
     6121 ttgctgaaaa agctggattg catagaactt atatttgtgc tattgaaaga aaaaagagaa
     6181 gtattgcttt agataatgtg cagaaaattg ctgatgcgct tgagattgag acttacttac
     6241 tatttataga aggtgaggag aaagaatgtc tatgaaaaaa ttaccaatag aaaagcttat
     6301 agaaaattta ggtttttcag ggtcaaagaa tcttgtgaat atagatatgc ttacaaaatt
     6361 agatatatct aaacatgatg taaaaacttt aaaagttata aatccttatg caatttattt
     6421 agtaaatagt aagccattta tattatttat agagtcttct tttgaagaaa aaaaattaca
     6481 acaaattagt aagcttatat ggaatgctca aataccaatt gcttttatat gcgattctaa
     6541 ttctgttaaa atatttaatg gaaaagcttt ggatttaaag tcatatttaa ttgaggaaat
     6601 atataatcaa tctattgagg atttttcgga agaatctgag ttttcttatt tgaaaatatc
     6661 tgatccatta ttttgggatc aatacactaa acaatatacc aaaacgcatt taaatgatta
     6721 tttgctagat aatattactt cattgacaaa taaattaaag aaaaaatata aggtgaaatt
     6781 tgcaacgaag cttgttctaa gattaatatt tattagatat ttaatcgata gaggtgtcga
     6841 tatagcttat aaaaatttta atcatgatat taaagcttca caaaaagaac tgctaaagat
     6901 tactaaaaac aaagttgaat tatatgaact gtttaaatac ttaaaagaaa agtttaatgg
     6961 taatttattt gagttaaatg acgaaattaa taatacttgt ttaacagatg aagtgtttat
     7021 tttactatct gattttttat caggtaaaat aagtattgac aatggacaaa tgtctttatt
     7081 ttcaatgtat gactttaata tcataccagt tgaactaatt agtaatatat acgaaattct
     7141 tttaggaaaa gaaattcgta ataaagataa cgcattctat actccgaatt acttagcaga
     7201 atatatttta gataaaacaa cacttgaatt tctgaaaaat aataggcaat acaaaattct
     7261 agatccttct tgtggatcag gagtattttt agtaaatagt tttagacgta ttattgatat
     7321 gaatttgggt gataaacttt tttgtgaaga tgataaactt ttaaaagaac ttttattatc
     7381 taatatttat ggaattgata tcaatgaaga tgctatcgat gttactattt tctcgttata
     7441 tctaactatt ttagattaca aagaccctaa gatattatca gaatttaaac tgcctaattt
     7501 aaaagggtgt aatttaatag cgagtgattt ttttgatgat aataaattgg agagtttaaa
     7561 gaaaattaac tttgatttta tcattggaaa tccgccttgg ggaagtgtct cagatggatt
     7621 acatttagat tattgcaata aaaatggcta taatgatatg caacaaaaca aagaaatcag
     7681 tagaagtttt atttttagag caaaagattt tagctctaga aatacagtat gttcttttgt
     7741 agtgcattct aaaatactat atactcaaaa aaagccatca aaaaaattcc gtgaattttt
     7801 gcttaaaaaa agtcaaatat ttagtgtagt tgaaatgtca tctgtaagaa aattagtgtt
     7861 taaaaatgca gacgctcctg cagcagttat atcatttaag tataacgaaa gtgaagaaaa
     7921 tttaaataat agaatcatct atacatcttt aaaaccaaat gtatttttta aattatttaa
     7981 cattattgta attgaaaaat atgatgttaa atatatttca caaaaactat tattggagaa
     8041 tgattgggct tggaaaacta ttgtgtatgg attcagtaaa gatatagata taattatgaa
     8101 tatcaaaaaa aaatatcaaa ctgtaaatga aattattttg aaaaacagtt ttgttcatgg
     8161 aacagggata caaatcaatg gtaatgaaag ccatgatgcg acacatttaa aaggaaagtg
     8221 gtatgttgat tcaaagaaag gaatagattc attctatgtt gatataaata aagggaaaga
     8281 acttactcaa gataaaatga gaagacctaa ggctaatcag caagaaatat ttattcctcc
     8341 atatgtatta ttaaaaaagg gagttgacac gaaaacatat agatatagag cagcattttc
     8401 aaatgatgca tttgtatatt cagatgcagt tacaggaata tgtggaaaaa aagataattt
     8461 aactacactt tattgcttga ctggtatgct taactcttct ttttactcat acttaaatat
     8521 aatgctaggt acatcaattg gaatagaaag agaacaagga ttttcaacgg aaatttttac
     8581 gtatccagca atagaaagtg atgaaattgc taatctggta gtaaaaatac aagaagcgac
     8641 taaattagaa aaaacaacta tgttcttgaa tgaaaaaaat agttgtaagt atatcaaaca
     8701 attagataag ttaattttga agtcttttaa tttaaatgat gataaattta ttgactatgc
     8761 aataaatatt caaattcctg agcttacaaa ttctaagtca actaacatat atagaaaaat
     8821 aactaaggaa gatttaatta attatagtga atgtttcaaa gaacagtttt catcgatata
     8881 tcgtagggtt gggaaaagta tttcaatttc ggtatatcca aaaatattaa atcgttttac
     8941 agtatttgaa ctagaagtaa cagatgaaaa aacagataga gagattatgg ttaaagcaga
     9001 agctgataat aatatggagt tgtttacgaa aataattaga tatgattata atgatgtttt
     9061 tcatcaaatt agagatatta ttcattttgg agaaaattca ttttatatca taaagcctaa
     9121 ttgctataaa tattggcatc ctgctatcgc agaaatggat cttgctgatg ttttggatca
     9181 gattttatct gatacagggg gtgaagaata atgggaggat ttaaaaattc ttcactaaac
     9241 ttttatttaa agcaagggct ttatgaaata attatagatg gattaataga ctgttgttta
     9301 cttatgagca aatcatgtat tgaacaatct agaaaaatat taaatcacga agaaaagata
     9361 agaacgcatt tagttgaaaa ttatttagaa aatgatattg aaagaagaaa aacatcttta
     9421 tctaaacaac atctaagatt tgaagtagaa gcacaagaaa attatgacaa caaaaatgaa
     9481 tcttatgagg gtagggttga tataaaagtt atatctaaag acactttagc ggatagaagg
     9541 gcttattata tcattgaatg taaaaggata gatggtaagg aaactttaaa taaaaaatat
     9601 ataaccgaag gtgtacaacg atttattact gagaaatatt cttcgcatta tggcaaaaat
     9661 ataatgcttg gatttatagt aaaagatata aatatccaga gcaatataca aaaaattaat
     9721 tcactgaatc agaacatatt atatacatat atagatacag atatgttgac agttagaagt
     9781 gattctgaaa ataattatgc tgtttgtgaa agtaaatata aagtaaaatc taaactacta
     9841 ttgttaaatc atatttttta taacttttcg agtataataa gataatctta tgaatataaa
     9901 atattactat gttaaaatta tagtttggtt ttgataatat tataaaaata agtttaagaa
     9961 taaaatataa aggaaaaaag gggcgtagca ctatttttta gtgtgacagc ccctttaatt
    10021 ttaaaatcgg taaggaggag tgtatttgaa aaaaattaag tcatatacag ggattttgaa
    10081 tgtggagaag gttatatatg caataaatga ttttcagctt ccatttccag ttacttttac
    10141 gcagatgaca tggtttgtat taacactttt tataattata ttatttggta agttacctcc
    10201 attatttatg atagaaggag catttttaaa gtatcttggc ataccaattt ttataactta
    10261 gtttatgtct caaaagactt ttgatggaaa gaaaccatat ggatttttaa agtcaataat
    10321 actatattca ttaagaccta aaataacata cgcaggaaaa aacattaaat ttaaaaataa
    10381 aattattgat gaaaatataa ctatagtaag gagtgaatta tatgttcccg ataaaatata
    10441 tagagaacaa tcttgtattt aataaggaag gagaatgttt tgcatattat gaattaatac
    10501 catataatta ttcattctta taagctgaac aaaaatttat tgtacatgat aattttaggc
    10561 aattaatatc acaaagtaga aatggtaaaa ttcatgcact tcaaatagcc actgaaagtt
    10621 ctataagagt tacacaagaa aggttaaaga aaaatattac aggaaaatta aaagaaattg
    10681 cttatgaaaa aatagacgag caaacagagg cacttgtttc tatgattggc gataatcaaa
    10741 ttgattatcg tttttttatt gggtttaagc ttttagttac agagaaagaa atgaatataa
    10801 agagtataaa aaagtcaata ttagctaatc tatcagattt tatatatgat gttaataata
    10861 aattgatgga tgactttgtc agtataaata atgatgaaat tatgagattt ttaaaaatgg
    10921 aaaagttatt agaaaataaa atctcaagaa gatttaaagt tagaaggtta gataaaaatg
    10981 attttggata tttaattgaa cataattatg gaaagaaagg cacagttttt gaagattatc
    11041 aataccattt acctacaaag aaacttaaaa gagaaacatt agtaaagaaa tacaatctta
    11101 taaagccaac tagatgtctt atagaagaga agcaaagata cttaaaaatt atacatgagg
    11161 attcagaaag ttttgtagct tattttacta ttgaaattat tattggagaa atggattttc
    11221 catctagtga gatattctat tatcagcaac aacagtttgc atttcctatt gatactagta
    11281 tgaatgtgga aattgtaaca aataaaaagg ctttaactac agttagaaat aagaaaaaag
    11341 aattaaaaga tttagacaat catgcttggc agtcagggaa tgaaacagta acaaatgtag
    11401 tagaagcatt agaggctgta aatgagcttg taaataattt aaatcaaaca aaagaatcaa
    11461 tgtataagtt aagctatgta attagggtat cagcagatag cattgatgaa ttaaaacgcc
    11521 gttgtgatga agttaaagat ttttatgacg atttaaatat aaaattagta cgcctaatgg
    11581 gagatatgat aggatttcac aatgaattta taccttcaag caaaaggtat ataaatgatt
    11641 atattcaata tgtaactagt gattttctag caggattagg gtttggagca actcaatagc
    11701 ttggagagaa cgaaggaata tatattggat atagtttaga tacaggaaga aatgtctatc
    11761 ttcaaccatc tcttgcaagt caaggagtaa aagaatctgt aactaatgcg ttggtagcag
    11821 cattctttgg atttttagga ggtgggaaat catttgctaa taacttactt gtttactatt
    11881 cagtattgtt tggagggcaa gctgttttgg tggatccaaa agctgaacgt ggcaaatgga
    11941 aagaaaattt accagatata tatcatgaag taaatattgt taatttaaca agtgatatag
    12001 aaaataaggg aatgctagat ccttatgtaa ttatgaaaga agtaaaagat agtggaagtc
    12061 tagctattga tatattaact tttcttacag gaataagtag tcatgatgga gagcagtttc
    12121 ctatacttag aaaagcaatc ataaatgtta ctcaagacaa atgcagagga cttttgaagg
    12181 taatagatga attaaggaaa gataattctc aaatatcaaa taatattgca gatcatattg
    12241 aaagttttac agattatgat tttgcacaat tacttttctc agatggaaat attaaaggct
    12301 caattaatct agaaaaacaa atgaatataa tacaagttgc agatcttgtt ttaccgaata
    12361 aagatacttt atttgaagaa tatacaacaa tggaaagtct ttcagtagct atgttgatag
    12421 taattagtac ttttgcatta gattttatcc atacagatag aagtactttt aaaatagtag
    12481 atttagatga agcttggaca tttcttaatg tagcacaagg aaaaacttta tctaataaat
    12541 tagttagagc aggatgtgct atgaatgcag gagtgtattt tgtaactcaa aatgcaggtg
    12601 atgtatcaga tgaaaattta aaaaacaata ttggattaaa atttgcattc agaagcacag
    12661 atatcaatga aattaagaga atacttgaat tttttggagt tgataaggaa gatgagaata
    12721 atcaaaaaag gttaagagag ttagagaatg gacagtgttt aatacaagat ttatatggac
    12781 gagttggggt aatacaaata catctagttt ttcaagaact ccttactgca tttgatacaa
    12841 gaccacctat aagaaaagag gtggattaat gaaatcgaaa gtatttagaa atttaagctg
    12901 gataactatt ttaatagtag tacttttact attttttatt tatataaatt tatagattta
    12961 gagaaacaaa tagatacatt agataagaaa agaaaaaaac tcatagatat gagattagaa
    13021 gatataattg ataaagaatc atatgaagaa aagtatgatg atttaacagt tgaaataaag
    13081 aaacttagta aagagaaaaa aactcttaaa gcatcattaa taaatgaaaa gagtataaaa
    13141 gcaagactta gagaatttag aaagatactt gaaagaaatg aattgttaga aaaatttgat
    13201 agatatgtat ttgcaagtat agtagataag gttatagttg gagttattaa tgaagatggt
    13261 atagttgatc cttataaaat aacttttgtt tataaaacta gctttgatga taaacaagat
    13321 ggacataaat ttataaataa gagtccgtta gaaacgtatt caaaagataa caacgataat
    13381 aaaaagaagt gttcatatac taataatgag acattgcgag actgtagtta aacttattag
    13441 agaataacaa ggtttcagac ttttatattg tgtcttaaaa gtagatttgc cacctatttg
    13501 ccaccataat tatttttggg tggcaaattt attctacaat gatttcaaaa atatttaccg
    13561 tctggtttct catgttgtta gttacatgta aatatgtatc catagttgtt gaagttttag
    13621 catgtccgag tatatgctgg atatctttta tattagtacc attttctaat aacatagtat
    13681 cagggttttt aaaataaagt gtgtattctt ctaaagtagt gataattaaa atatcaaata
    13741 tcactataca aaggaaagtg cacgcttttt atgtttaaca aaaacgaaat attaaaaaac
    13801 ttattgaaga atatgatgtc aaaacaacta cagatattca agatatgcta aaagatttat
    13861 ttacaaatac aattcatcaa atatttacat tgtcgaattt tagcggttta aacataatat
    13921 attgactgat ataataaaat catagaatat tttaggggga aatagattat gtttaagaaa
    13981 aagttaatag ctgttgcaat gagtataact atgattagtg tagggagctt ttcgtatgct
    14041 cattcaggaa gaactgattc atcaggaggt catcgtgata ataaaaataa atctggatta
    14101 ggaagttatc actatcattg tggtggttat ccagctcatt tacataataa tggtggttgt
    14161 ccttatactg gaggaggaag cagttctgga acaacaactt cggttaataa tgaagaaaag
    14221 caaaagaggt ctgttggaga aaaaggatat aatcaagggt atgaagatgg gtataaagga
    14281 aattatagtt catcaaatta tagtggagat tatagtgata catatgaatc aaagtactca
    14341 gaaggatatg aaaaaggtaa ggctaaatta gaagaggaag aaaaggtggc taaagagact
    14401 ggatataatt tagggcttac aggtgctaaa tctaataata catatgaaaa agaagtttta
    14461 aaaaatgctt atgataccgg atattcaact ggatacaatg aatataagac taaaaagata
    14521 gaagaatata aagctaaagg gatagaggat agcaataaag ataaggaaaa aatgacattt
    14581 gaagaaaaca tagattctga atttatagat gcttataata atgcttatga cgaaagacaa
    14641 gaacaattaa aaaatgatta cactactcaa ggatttgaga gtgctatcaa aggagaaaga
    14701 tttgacacat caactatagg taatgttaaa tatgctaatt ggtttaaaga aggatatgat
    14761 aatggtaagg ttaagttacc taaagcaaaa gaaagtgtat ataatcaagg ttacaatgaa
    14821 gaagatttta gtataccaga tgaaatgaag tctattgaaa ctaagttaaa aggtgtatat
    14881 gatgaaggtt tagaaaaaag agaagaagag aaaagtagaa atgctactta tggggttgga
    14941 gctggaacag cagctgtagt tgctggagta gtatataaga agaaaaaacg taaaaagata
    15001 tagatgctag atataccaat gatgacaagt gtataacaaa tataatttta agtataaata
    15061 tttaaaatat agaagggaag gttatatcta tgggatttaa ctttaggaaa agcataaatc
    15121 taggaggagg actcaaatta aatttaggta agaagagtgt aggtataagt gctggtgtta
    15181 aaggtgctag agtgtcagtt aattcaaaag gaagaaaatc agctacattg agtatacctg
    15241 gaactggaat atcatataca aaaactagta caaagaaacg taaacctaaa taaatatttg
    15301 atatactaag aaatagactg ttacaaatag tctatttttt agtatataga tttaaaaagt
    15361 gacaaaattt tgaaatataa actaatttca taaaggaaaa tattatttta tgtagaatta
    15421 taatatttaa gtaatattat aaaatttata ggaggatttc attatgaaat taagcaaaat
    15481 actattggca atagggatgt ctgtttcatt attgattgct agttgtccaa catcaaacgc
    15541 tcttagttct attaattcaa taaaaggtac aaataagtat gaaacagcag gtttaatagc
    15601 agataaacaa aattatgata ttgcaatact tattaattct gaaaatggat tatctgatgg
    15661 attgtcagca agtggattgt caggagctaa aaatgcccca atattgttaa ctaagaaaga
    15721 tacaatccct tcagaaacat taaaaagatt agaaaaggta aaagaagtat atctaattgg
    15781 aggaaaaaat tcgataggag catcagttca gaaaatatta agtgataaaa aaataagcat
    15841 aaatagaata agtggaactg atagaataga tacaagtcat aaagttgcaa aagaaatagg
    15901 aagagtaagt acggttaaaa aagtaatatt aactaatgct tttaaaggcg aaccagatgc
    15961 tatgagtgtt gcttctatag cagttagaga taaatctcca atagtattaa ctgatggaaa
    16021 tactacaagt tttgctacag atggattaga aacttatgca attggtggaa aagtcagtat
    16081 gagtgataag ttaataaatg aaactaaagc aacaagaata ggtggagtag atagatacga
    16141 tacaaataaa caaataataa ataaatttta taatggctca aaagaatttt acatagcaag
    16201 tgggactgat ttagtttatt ctttagttgg ttctacaatt gcacaaaaaa cacctatagt
    16261 gttagttgga aatggaagta ataagagtat attaaaaaat gcaagtaaaa taactgctat
    16321 tggaaatatt gatgatgttg ttataaaaca atgtttaaat ataactaata atgttgggga
    16381 ttcaaataca ggtattgtaa aagatactaa ttcaaataat acgaacaaac caagtaatga
    16441 gcctagctct aataatcctg gattaactga taaaagtaca gtatatgtaa ctcctaatgg
    16501 aaaatcatat catctaacaa aaaattgttc tacactaaaa agaagtaaaa caataaataa
    16561 agtaacatta aaacaagcta agtcacaagg taaaatagat ccttgtaatg tttgtgtaaa
    16621 ataatgtatt agccaaatat atattaaaaa agacaattaa ttaagattgt c
//
LOCUS       DAEOSP010000071        16592 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.071, whole genome shotgun sequence.
ACCESSION   DAEOSP010000071 DAEOSP010000000
VERSION     DAEOSP010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16592
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.071"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            109..1482
                     /locus_tag="KRH54_001878"
     CDS             109..1482
                     /locus_tag="KRH54_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8579536.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRKNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            1738..2805
                     /locus_tag="KRH54_001879"
     CDS             1738..2805
                     /locus_tag="KRH54_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBG8579537.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     NVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            2926..3234
                     /locus_tag="KRH54_001880"
     CDS             2926..3234
                     /locus_tag="KRH54_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBG8579538.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3227..4657
                     /locus_tag="KRH54_001881"
     CDS             3227..4657
                     /locus_tag="KRH54_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBG8579539.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKAIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            4735..5109
                     /locus_tag="KRH54_001882"
     CDS             4735..5109
                     /locus_tag="KRH54_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBG8579540.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5103..7307
                     /locus_tag="KRH54_001883"
     CDS             5103..7307
                     /locus_tag="KRH54_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG8579541.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7307..8716
                     /locus_tag="KRH54_001884"
     CDS             7307..8716
                     /locus_tag="KRH54_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBG8579542.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIKYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8701..9765
                     /locus_tag="KRH54_001885"
     CDS             8701..9765
                     /locus_tag="KRH54_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBG8579543.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFSNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            9795..11216
                     /gene="nhaC"
                     /locus_tag="KRH54_001886"
     CDS             9795..11216
                     /gene="nhaC"
                     /locus_tag="KRH54_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBG8579544.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            11645..12109
                     /locus_tag="KRH54_001887"
     CDS             11645..12109
                     /locus_tag="KRH54_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG8579545.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12343..12705
                     /locus_tag="KRH54_001888"
     CDS             12343..12705
                     /locus_tag="KRH54_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG8579546.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            13300..14136
                     /locus_tag="KRH54_001889"
     CDS             13300..14136
                     /locus_tag="KRH54_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8579547.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLAFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     LS"
     gene            14220..16334
                     /locus_tag="KRH54_001890"
     CDS             14220..16334
                     /locus_tag="KRH54_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HBG8579548.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMATKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHVVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REKYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELTQLIDM"
BASE COUNT         6571 a         1846 c         3103 g         5072 t
ORIGIN      
        1 aatatatgat aagataaata caatagcaac attttattaa ttttttgggt ttttatatat
       61 ttaggtactg gggtgatata gttgaaccaa gagatatttt ttaaaataat gaatgaaata
      121 ttggaaaata ttgatgaagg ggtacatttt gttaataaag acgggaaaac tataatctac
      181 aataattctg tacaaaaaat ggaaaaaatg aacagacacg atattttaac caattcattt
      241 tttgatatag tcaacaaaat gaaaataaac aggagtacac tattagaagt tttggaaaaa
      301 agggaagtta tcaaagacaa tattcaaaaa tatctggata aaaatggaaa agagataaca
      361 gcaattaata ctacaatacc aattgatatt aaaggcgagt ttgtaggggc acttgagata
      421 tcaaagaata tgacaacgat agaaaattta ctaaatagaa attataaaaa aaatttagtc
      481 aataaagtta gtagtagaca aaaaaagggt tatgaatttg atgatatttt aggggaaagt
      541 aatttgatta aagaagcaat agctaaatct aagaaagctt gcaagtctga tgcttcagta
      601 tttatatacg gtgaaactgg aacgggtaaa gagttattta gccagtccat tcactatgga
      661 agtaatagga aaaactatcc atttatagca caaaattgtg cagctttgcc ggaatcacta
      721 ttcgaaggta ttctttttgg aactagcaag ggaggattta caggagcaat agataggcca
      781 ggcttatttg aacaagctaa caaagggaca ttgttattgg atgagataaa ttccatgcct
      841 attatgttac aggctaaact attaagggtt ttacaagaag gttatgtaag aagggttgga
      901 ggaactaagg atatacctat agatgtaaga attattgcaa cgacaaatga aagtccaaag
      961 gtaattttaa acgagcataa aatgcgaaaa gatttatatt ataggcttaa cataattcat
     1021 ataaaaattc ctcctcttag agatagaaaa gaggacatac ctctattgtg caaaaaattt
     1081 atttcaaagt ataataaaaa gttaaataaa aaagtggtag gaatttcaga tgaagctatt
     1141 aaaattttaa aaaattacga ttggcaaggt aacataagag agctagaaaa cataatttat
     1201 tgtactatga gtatgatgga agatgaggta caaatagaaa caagtatgat taatctaaac
     1261 gactattaca atcttaaaga gaataaagag agtaacaatt attctttaaa cgatttggag
     1321 gacaaaacac ttagtaagat tatcagtgag atagaagaaa agtatatata tgaatcatta
     1381 gaaaaggcat cttacaatat aactaaagct gcatcaattt taggtatgaa taggcaaaat
     1441 ttacagtata aaataaaaaa atataatatc aaaatattat aaataagcaa gataaaaaaa
     1501 caaaatcaaa gtaaaataaa aaagcaaaat agaggagcaa aattattata aaagagcaaa
     1561 atattttgct ctttttttta tttctcataa gaaatattat tttttattta tgaaaataca
     1621 taataaaata attatagttt caacgttcca ataaatattt actttaattt taaagttggc
     1681 acgatttatg ctttaactaa ttattgagaa tatataaatt tttaggaggg tgtatatatg
     1741 agaaaagtaa gagtaggaat atggggattt ggagctatgg gaataggtat ggctaatatg
     1801 attctaaaga aagaaggtat agaaatagta tctgtttgta gtagaagtac ttcaggaaaa
     1861 agtatgtata atgttttagg aattgaaaga ggagaaagac cagaagttat tatcaataaa
     1921 aattatgaag aagtttttag agaaaaaagt gtagatgttg ttttgttagc aactgattca
     1981 tttactaaaa aggcttttga taaaataatt ttcttattaa atagaaaaat aaatgttata
     2041 tcaacagcag aacaaatggc atatccacaa gctgatgatg cagatttagc taaaaaaatg
     2101 gatgaagttg caaaggaaaa tggagtaagt atacttggaa caggaataaa tccaggattt
     2161 gtattagatt tactagtatt ggcactttca ggtacttgtg aagaagttac tagtataaaa
     2221 gctaagagag taaatgattt atctccattt ggaaagtctg ttatggtgga acaaggtgtt
     2281 ggagtaacaa gggaagaatt tataaaaggt gtagaagata agactatagc aggtcatgta
     2341 ggttttgtag aatcaataaa tatgatagct gatggattag gttggaaatt agataagata
     2401 gaacaaacaa aagagccaat aatgactaca gtagatagaa aatctaagta cggagaggca
     2461 ttggctggaa atgttgctgg ttgtagacaa tgtggatatg gatatgtaaa tggagaagtt
     2521 ttaattgaaa tggagcatcc acaacagata ataccagaag cagaaggtat taaaacagga
     2581 gactatgtat caataaaagg tataccaaac attgatttac aaataaatcc tgaaatacct
     2641 ggaggagtag gtacttatgc aatgatagta aactctattc cattaataat aaatgctaga
     2701 ccaggtctta aaactatgtt ggatatacca gtacctagag caataatggg agatataaga
     2761 aatcagatag aagttgaatt ggaagaagaa tcaaaggcta attagtaaga tagcaactag
     2821 ctaattagta agataacagt tagctaattg gtaaaataat gtttaattta aaataacagt
     2881 aaattaatta gtaaggcaat agtcaattaa taaggggatg aaaaaatgga tgctaaaatt
     2941 aatgattggg ttattataca caatatagta ttgactccag aagaaagagc tccacaagtt
     3001 ccagaggata ctaagaaagt atccttagaa atgtgggtta aaggttttat acaaagtgat
     3061 gcttctatag gcgacttagt agaagttaaa acaattacag gcagacttgt aaaaggtaat
     3121 ttacttaaaa taaatccata ttatactcat gattatggta aatgtatacc agagttactc
     3181 caaataggaa tccaagctaa agaaatatta tttggaggtg tatataatga ataattcaac
     3241 atcaatgaaa gatatgagtt atcaagctgt tatgggaaga aataacgaaa taatgaaaaa
     3301 tgcaatcgga ttggattatt cttcttttga acaagaggga atagggtttg attatgagaa
     3361 aatgatgagt gaaacaggat atacattaca agatattgaa gcaattcaat cacagtatgc
     3421 tgttggaaat actcctttaa tagagcttaa aaatttgact aaattagcta gaaaatgtgc
     3481 taaagaaggt aaaggagcta gaatatttgt aaaagatgaa gcaatgaatg catctggtag
     3541 ttttaaagca agaagagcag ctactgcagt atatcatgcc aaacaaatgg gatataaagg
     3601 tgtaatagca gctactagtg gtaactatgg tgcagctgta gcatcacaag cggcaatgca
     3661 gggattaaaa tgtataatag ttcaagaatg ttatgattct aatggtgtag gtcaacctga
     3721 aatcatagaa aaagcgcgaa aatgtgaggc atatggagca gaagttgttc agttaactgt
     3781 tggaccagag ttattttata cgttcttagt tttattagaa gaaacaggct attttaacgc
     3841 atcattatat tcaccatttg gaattgcagg tgtagaaact ttaggttatg agttagctat
     3901 ccaatttaga gaaaagtata aaaaagaccc tgatatagtt gtttgtacaa atgcaggtgg
     3961 tggtaatctt actggaactg caagaggatt gataaaagct ggttctatta atactaaagt
     4021 agtaggagca agtgtagatt taaaaggatt acacatggct agtgataatc aatttaataa
     4081 aaaatcattt acaacaggac atacaggttt tgggatacca tactgtacat ggcctgatag
     4141 gtcagatgta cctaggtctg cagctagacc acttagatat atggatagat atgtattagt
     4201 aaaacaagga gaagtttttt atacaactga attattggca cagctagaag gtatagaaag
     4261 aggtccagca ggaaatacat ctttagcagc agcattctct ttagctcaag agctagatga
     4321 ggataaagct atagtggtac aagaaacaga atatacgggt gcaggtaaac atatatgtcc
     4381 acaacttacc tttgcaagag aaaatggaat agatataaaa tttggaaatc caagagaaga
     4441 aattgcagga gtaaacttaa tattaccaga aagaccagaa ttattgaaat gtgttgatat
     4501 tgatatgaat aaaataagaa agtcatttat aaaaaattgt atatctaata atcacattga
     4561 taatattgat aagctaagta atcgagatat agaattttta atggaagaag taaaaagttc
     4621 aagagatttt gttgttgatg tattaaataa tttatagtaa ttattgtttt ttaatttaat
     4681 ataaaataaa aattcagaaa attcgattaa atcaatacga atggaggaat gttcatgaaa
     4741 aaaagagagg atgattttga agttagaaga aaacatcttc aagaactgag tgatgatgag
     4801 ttaaaagaaa ggttttggag tttagctacc cagatagtag aaccaatgat agaacttggt
     4861 aaaaagaata caacaccatc aatagaaagg tctgtcttgt tgagaatggg attttcttct
     4921 cttgaagtaa aacctatttt agaaggggtt atggaaagag gtcttatagg taaaggagca
     4981 gggcatgtag tttataaatt ggctaaatct aaaaatataa cggttagaga agcaggtttg
     5041 ttattagtta agggtgaata ttgggatgaa gttacatgtt tatttagaga aggggtagaa
     5101 tcatgttaaa ggaaaataaa aaattggata tagactatat attaaaagat ttggataaat
     5161 ataaaccaaa acgaagaggt tgggtttgga gagaacactt ggaaaatctt gaaatgggac
     5221 cttttaaata taaagactgt acaaaacctt taaagaagag tgtagggcta ccatcatcta
     5281 agtatttcaa taatatagac cctcaaccaa gtccagttat tactactgaa atagcttctg
     5341 gtagatttga ggatgatata agaagaatga gaatggcagc tcatcatgga gctgaccatc
     5401 taatggttat cagaactgct ggacaatcac attttgatgg gcttatagaa ggaactccac
     5461 aaggtatagg tggagttcca ataacaagaa aacaagtaag agctcaaagg aaggctcttg
     5521 attttataga agaagaagtt ggtagaccta ttaattacca ttcatatgta agtggtgttg
     5581 ctggtcctga agtagcagta atgtttgctg aggaaggagt caatggagca catcaagacc
     5641 cacaatataa tgttctgtat aggaatataa acatggtaag gtcttttgta gatgcttgtg
     5701 aggcaaagaa aataatggct tttgccaata tagctcaaat tgatggagca cataatgcta
     5761 atgctactgc tagagaagca tggaaggtaa tgcctgaact tatggttcaa cacgctttaa
     5821 actctatatt ctcagaaaaa ataggtatag ataaaagtaa tatatgctta tcaacagttc
     5881 caccaacagc acctccagca ccatgtttaa aaatagactt accttacgcg gttgcactta
     5941 gagagttatt tagtgattat aagatgagag ctcaaatgaa tactaaatat atggaatcat
     6001 caactagaga agcaacagtt actcatgtat taaacttatt aacatcagta ttaactagag
     6061 ctgacataca atcaactata acacctgatg aaggtagaaa tgtaccttgg catatataca
     6121 atatagaagc atgtgataca gcaaagcaag ctttagttgg tatggatggg cttatggata
     6181 tgatagaact taaagatgtc ggtgaattaa gagacaaagc aagagaactt aaagaaagag
     6241 ctgtattata tatggaagaa atatctgaag ttggtggata ttttgaatct gtggaacaag
     6301 gattttttgt tgattctgga aattatccag aaagaaatgg tgatggaata tctagaaaaa
     6361 taaaaggtgg agttggtgaa ggtactgttt atgaaagaga agaagattat ttagcacctg
     6421 taactgctca ctttggatac aataatgtag ctcaatatga tgaaaatgct atagataatc
     6481 catcaatgtt aatagatgga tgtacatttg aaaatccaga caaaataata tatattgatg
     6541 agcttgacga ttttgataat gtagaaaata gactaaaaga atcatatgag tacagaaatg
     6601 gaactaaaat taagcctgaa atggaatggt gtgcagatgg aattgtaatg ataacaatga
     6661 tgcttcctac tgataaaaga acagcagagt ttgcagcttt ggaatttgtc aaaaaaatga
     6721 acttacaaga aattgaggtc ataagtagag aagttatgca tgaatctgaa gggactagaa
     6781 tagaagtgaa aggaagagtt ccgtttgata tagatttaaa caatttagta ataccagaag
     6841 aacctaaggt actaactgat gaagaaataa gagcagatat agaagaaaaa cctatgaaaa
     6901 tagtttcagc tacggttgga gaagatgaac attcagttgg tttaagagaa ataatagata
     6961 taaaacatgg aggtattgaa aaatatggca tagaatgcca ctatctaggg acttctgtac
     7021 ctgtagaaaa attggtagat gcagcgattg aactgaatgc agatgcaata cttgcatcta
     7081 caataataag tcatgatgat attcactata aaaacatgaa aaaattacac gattattgtg
     7141 tagaaaaagg tataagagat aaagtaatga ttgcgtgtgg aggaacacaa gtaacacctg
     7201 aaatagctgt tgaacaaggc atagatgctg gatttggaag aaacagcaaa ggcatacatg
     7261 tagccacttt cttagttgaa aaaagaagag aaatgagtaa taaataatgg aaaatagcaa
     7321 ggtttcttta aaaatagatg tgttggtagc tgaaattgga agtactacta cagtggtaaa
     7381 cgctttccac gatattaata ctaacaatcc tatatttttg ggtcaaggtc aagctccaac
     7441 aacagtgttt gaaggtggcg atgtaagaaa tggcttatca ggtgctatta aagatttagc
     7501 taataaatta tctgtagatg atataaagta taatgatatg tttgcaactt ctagtgcagc
     7561 tggtggatta aaaatgactg ttcatggatt agtctatgat atgacagtaa aagcagctaa
     7621 agaagcagct ttaggggctg gagctgttat tcgtcaaata acatcaggta gaattaaaag
     7681 aactgattta aataaaataa aagaaataaa cccaaacata attttaatag ctggtggagt
     7741 cgattatgga gaaagagata cagctattta taatgcagaa atgatagctt ctatgaatct
     7801 aggcatacca gttatatatg ctggtaatgt tgaaaatcaa gaggaaataa ggttaatatt
     7861 tgaagatact aattataagc tttatataac tgaaaatgta taccctaaaa tagatttatt
     7921 gaatatagaa cctacaagaa gaatcattca aagtgtattt gaagaacaca taactacagc
     7981 accaggaatg aaatacatta aagaaatggt taatgaaaat attactccaa ccccaggagc
     8041 tgtaatggaa gcttctaaac ttttatataa aaatattgga gatttattaa ctctggatgt
     8101 aggtggagca actactgatg tacattctgt tacagatgga agtgactata ttaataaaat
     8161 tttggttaat cctgaaccta ctgcaaagag aagtgttgag ggggatttag gtgtatatgt
     8221 aaatatgaag aatatagtag aagttatagg taaagaaaac ttacaaagtg aactatctat
     8281 agatattgat gcagtaatag agaattatcc tccaatacca aaatcaaaag aagaaatatt
     8341 atttgttgaa agattgacaa aagaagctgt tgttaaagct atgctaagac attcaggaaa
     8401 aataagaaat atctatggaa caactggaaa agtcaaaatt gcagaaggga aagatttaac
     8461 agaagtaaga tatatagttg gtacaggggg agcacttact aggctcccta gtagaattga
     8521 aatattagat aatatgctca aatacaataa aaataatgaa ttactatttc caaaggaaaa
     8581 aacaaagata ttaatagata atgactatat tatggcatct atgggtgttt tatctaaaaa
     8641 atatgaagaa gcatctctta agctcctgtt aaaaagtttg aattttgagg aggaaagatt
     8701 atgtatccta ggttagaaat agatatagaa aagttaaagc ataatgcaaa attaatttcg
     8761 cagatgtgtc atgaaagaaa tattaagata tcttttgtta ctaaatcatt ttgtgcacaa
     8821 aaagaaatag tagaagaaat atgtagtgaa ggaatagacc atatagcaga ttcaagaata
     8881 caaaacttaa aaaatttaca agatataaat ctacctaaga tattgattag aatcccaatg
     8941 ttaagtgaat tagaagaagt tatcaattat tgcgatatta gttttaactc agaattagga
     9001 acaataaaga aattaaatga attatgtgaa agtaaaaata ttattcataa gatagttctt
     9061 atgtttgact taggcgattt acgagaagga tacttttatg aagaagactt ttttagtaat
     9121 gtaagagaga tagtaagatt aaaaaatatt gaaataattg gtatagctac aaacttaacc
     9181 tgttatggag ctattatacc atctagggaa aatataggca gactggtaag tatagcaaaa
     9241 agaatggaag aaaaatttgg aataaatcta gaaatagtat ctggaggtaa ttcaagttct
     9301 attcatctgt tgataaataa taatatgcca gaaggtatta ctaatttgag aataggcgag
     9361 tccattctac ttggtagaga aacggcatat ggtgaaaata taaatggaac atatcaagat
     9421 gcatttaaat taatatgcca agtagttgaa tgtaaagaaa aaccatcagt tcctattgga
     9481 gaaataggag ttgatgcctt tagaaataaa cctgtttatg aagataaggg aatcttaaaa
     9541 agggcaatca tagctatagg taaacaagat ataaatattg actctttaat tccaatagac
     9601 actgatataa aaatattagg ggcaagttca gaccacatga tactagatgt aagtaacaca
     9661 aaatatgatt ataaattagg tgacaattta gaatttttat taacttatgg gggtatcatg
     9721 tcatctagta cgagcaaata tgtttcaaaa aaaattatag tgtagtgttt aaataattag
     9781 gaggaaacta ttttatgaaa gagcgagaat gtagaaaagc aagtctgttt gatgcaatga
     9841 taccaattac ttgtttgata ttatttttaa gtctaggagt attagtgtat ggttcatcac
     9901 ctcatgtacc actaatagga gcagcagcag tagcaggact tgtggctgta tatagacttg
     9961 gatttaaatg gaaagagtta gaattaagta tgtttaatag tataaagatg gcaatgcaag
    10021 caatcttaat cataattata ataggcgtac ttataggaac ttggatagta agtggagtag
    10081 ttccatgtat gatttactgg ggactaaaaa ttttatcacc aaatattttt ttagtagcat
    10141 caacattagt ctgtgcaata gtatctcttg cgacaggttc atcttggagc acaatgggta
    10201 cagtaggagt agctctttta ggcattggtc aaagtttagg tatgccaatt ggattaatcg
    10261 taggttctat aatatctgga gcttactttg gagataaact atcaccttta tctgaaacga
    10321 ctaacttagc tccagcaatg gcaggaacag atttgtttac acatataaaa tatatgctat
    10381 attcgactat accatcatta ttaatttgtt tagtaattta tggtgtaatt ggaatgaagt
    10441 attcaggtca agcactagat ataaaacaaa tagaattaat taggtctaca ttagattcta
    10501 catttaatac tttatcacca gtattattat tagctccagc aatagtaatt ggattggttg
    10561 tatttaaagt tcctgctata ccagggctta tagtaggtgc tgtattggga gtattgtttg
    10621 cagtagtttt tcaaggagaa tctatgaata cgatattaga tgcagcacaa aatggatatg
    10681 tttcgtcaac acaaatacag gaggtagatg ctttattatc taaaggtggg attatgaata
    10741 tgatgtccac tgtatcctta acaatatgcg ctttatctct tggaggtatt ttagaaaaaa
    10801 caggtatgct tgaagtagta gcatcttctt tactaagatt agcaaagggt gtatttggaa
    10861 cagtattatg tactatggta acttgtacaa ttaccaatat aatagcaggt gaacaatatc
    10921 tatctatagt tataccagga agaatgtata ataaggaata taaaaagaga ggtatacatc
    10981 ctaagatgtt gtcgagagct ttagaagata gtggaacttt aacctctcca ttagtaccat
    11041 ggaatacatg cggggcgtat ataacagcaa cattaggtgt atctgcacta acatatggtc
    11101 catacgcttt attaaatatt ataaatccaa tagtatcttt agtattaatt gcatgtaagt
    11161 ttaaaatagc aagaatagaa gatgaacctg aaacttgttt aaattttgat gtttgataat
    11221 agataactta tgtaagaacg taggaactag gggggtagcc tgtggggaat tagtaagaca
    11281 tattttagtg tgcgaaattc tataaatcag taatcttgtg actttagcca tgggaggttc
    11341 agattagtaa atttatagtt ttatattagt ctatattttg atcttttaat tgtttgtata
    11401 aattttttat agaaaaagat ttcttttaga cttgcctaaa aattaggcaa gtcttttaag
    11461 tatgttctga ttataagagc actgtgtgac aagaatgatg agattatttt tgctatactt
    11521 tcgatataag aaatggaagg aggttatgtt ttatattcat atgaaataat tagttacaca
    11581 gtaaattttc gtataatttg gtataaaaag attagaaatg aaaataaagt aatggaggaa
    11641 taaaatgact agaatatcca atataacaat agctgaacaa tctgagtact gttttttggc
    11701 aatacgaaaa acaattgact ttatggtaga gttttcagag ttttcaaaac aaagttttga
    11761 aaaaatttct aaatatctgg aggagagagg aattttatct tcaggagcac cgattgtatg
    11821 ttttcataac atggatttag caaaattgga tgttgaagta gggtttcctg tagccactta
    11881 tatagatggg aaaaatgaga ttctacaaag gattgttcct gcacagaaaa taataactgc
    11941 aattgattta ggaccttatg aattacaaga ccctacttta gaagacttat tttcatgggc
    12001 aaatagcaat ggatataaac ttcatggcga tatatattac cagtatttaa atgatatcaa
    12061 tcgtccagct agtgaatatc ttacgaaaat gatgttaccc attatataga aagttaaaga
    12121 ttatcgtttg ttagttgata attagaagaa catttactgc tactacataa attttatcct
    12181 aaataatatc aaggtgattt aatttatgta ttctaatgta accccaatat attgttaagg
    12241 tctacttaag atgctcaaaa ggctatgctt aatttattgt tatataattt gtagagtggt
    12301 ataatttaga ggtaatatta tatagatata tagggggaaa atatgaaaat atatgatata
    12361 ttaacacgtt tttatattca agatatagaa aaagccatac ctttttatga aaatctttta
    12421 aaagaaaaat gttctttgcg tttttcctat aaagaagttg ggttggaatt agcgcaaata
    12481 ggaaatgttt tactattatc tggttctgat gatgctctta aaccatttat agaaacaaaa
    12541 tctactttta tggttgattc tgttgatgaa tggagaagtt atctattgga taatggagca
    12601 gttgtagtaa gagataagaa aaaagttcct acaggatata atatgacatt gagacatcca
    12661 gatggtacaa ttatagaata tgttcaacat acaaagcagg attagttaaa aacagatttt
    12721 actactgagc aactaattag aatctattct tagacgtaca atttattatt tttggtatta
    12781 acagattata agtcaaatta taatatttca aaatagaata gaaatttatt tacataaaag
    12841 agagtgttta aaaatgaact ttttagttca tgaagcactc ttttttgtat gaaatcaaat
    12901 acatcaatat ataaaataaa ctgatgaaga gcttaaatta aaataaaaag attcataaca
    12961 ggaccccatt aaactttaag atagggtaag tttgtaatca tattaaataa ttgtaaaaag
    13021 ctgtttttag tcgtataata taaatattta tcatttaatt aaggaatata aaaggaatct
    13081 aataagaaga aaataataaa taacttttta gatttactaa atgaaaaatt attgaaaatt
    13141 aaaggattag agtctttaat cagaaagtaa aaagtaatat attaaattta tgaattgaaa
    13201 acttaaacaa caaatttgtt tataaaatag caagattcat taaatataac taagtaataa
    13261 ttgatataat gttgcatgga taaaaggagg gaagctgcaa tgtcttacaa agtaattaat
    13321 gtcataactg ctattgacta tatagaggag catttatcag aaaagctgga tttagatata
    13381 gttgctaatg ctgttcatta ctcaaaatat cacctgcata gaactttcac aacttctgtt
    13441 ggcctaacaa tgcatgatta cataaagcgg agaaagttga cagaagcagc aaaattattg
    13501 gcattttcaa agaaaccaat tattgaaatt gcattgatag ctggatatga aagtcaacag
    13561 gcattcactt ctatatttaa agctatgtat aaaaaatctc caaataaata taggaaagaa
    13621 caagaatttt atcctttgca gctaagattt gttttgaaaa aagataacct attttcaaag
    13681 aatgtactag agttggaaaa agaaattaaa cttgcaagta tgtctgacat accattatgg
    13741 atgaatttag tgagattagt catagatgga tttccaaatt tacaagagga agaatatata
    13801 tatcaattag aacagtatat tttagaagaa agagctttaa tattaaaact taataatgtc
    13861 gctattgcaa atatggtgtt taatagagaa actagaagta ttgatttttt tggaatacat
    13921 ccacagtatc gtaattcaga cattgcacaa gtgtttttga agaaagttat tgaagatttc
    13981 ttgatagata cagatatttc aattactacc tttcgagagg gtgacaaagc tgatacagga
    14041 catagagata tgataaagaa gttgggcttt gcagaagcag agttgcttgt ggaatttgga
    14101 tatcctactc aaaaatttat tcttccttta agttaaatag caagaatgat taaaaactcc
    14161 aaaacacaat taagtataat gattcagtgt aagtacttaa ttaacaaagg aggatttaaa
    14221 tgtctgaaaa taaacaagta gtaaattgcg atattattgc atctaattct aaacataata
    14281 tgtgtgaata tgatgcagag ttattaactt tttttccaga tgagattgca catctaatag
    14341 agataaataa caagctagac aatgctttta agaaagctga aaatcttgtt gacaagcttg
    14401 ataaggatta tatggacgct aaaatgtata tggtaaaaaa tcgtggtgaa atagacccac
    14461 atgaaatgtt tcaaaatgaa caaggactaa aacaaataga taactatggg gcttttatgg
    14521 taaaagttcg agataagatt aataaaatca aagattctcc atactttgct agaatagatt
    14581 ttaggctgaa agatatggat gatgaatcta agtattatat tggtagattt gcatttgatt
    14641 atgaagatga gcttataata cttgactggc gttctccaat tgccagtatg ttttatgatt
    14701 atgaaatcgg aaaagctgga tatgatgctc caataggatg ggtggatgga gaaataacaa
    14761 gaaaacgaca atttaaaatt aaaaatggaa aattagaata tgcactagaa agttccatta
    14821 atatacaaga tgatattctt caaaaagaac ttagtcacac ttcagatgaa aaaatgaagt
    14881 caatcatttc aacaatacaa aaggaacaaa atcaaattat tagaaatgat aaagctgata
    14941 cactgattat tcaaggggta gctggttctg ggaaaacatc tatagcatta cacagaattg
    15001 cttttcttct ttatcgattt aaagataaaa tatcagcaaa taatgtaatt atcctatcac
    15061 ctaataaagt ctttggagac tatatttcca atgtccttcc tgagcttgga gaagagccat
    15121 tatgtgaatt aagttttgaa aatatagcag aggtacaatt agatagagtt attaattttg
    15181 aaagtgagaa agacccatta gaaataaatg atgctaaatg gtctgaaaga gtgcgtttta
    15241 aatctacact tgattttgta aaacttattg atgattatat aaaacaaatg ccaaataaga
    15301 tttttattcc aaaagactac acttttggaa gttttacagc taaaagtgac tggatacaga
    15361 gtagatttga ggcatataac agatatccag ttaagaagag attagagaag gtagctgaag
    15421 acattcatta taaatttgaa tcagataata ttatggaaga agatttacca aaggtaaaaa
    15481 gtatacttaa aagtttgaat ggaatgttga caataaaaaa tactcttaca ctatataagg
    15541 atttctttaa acaaatgaat atatcaaaca tgtttgtgat ggcaacaaaa aaaactcttg
    15601 aatggtcaga tgtataccca tttatatata ttcatgctgc atatgaaggt atacaagaag
    15661 ataagattat aagacatgtt gttattgatg aaatgcagga ttacacacct attcaatatg
    15721 ctgtaataaa cttacttttt aagtgtaaaa agactattct tggagatttt ggacaattgg
    15781 ttaatcctaa tcacactcat acactagatg atatgaaaca gctatataat gatggggaac
    15841 tagttatgtt gaataagagt taccgttcta cttttgagat tattaatttc gccaaaaaga
    15901 ttcaagacat atcttcatta gagcctattg aacgacatgg tgaagaacca gctttattaa
    15961 aatgtaataa cgagcaagat gaaataaata agataaaaat agaaattgaa gaatttaaaa
    16021 agagtgataa tgcgactctt ggtattatat taaagacaga taatgacgct aaagctgtat
    16081 atgatgcgtt aagagaaaaa tatagtgttc acttaatatc ttctgaaagt tctagcttta
    16141 caaaaggagt atccataact tctattaaaa tgtcaaaagg tttagagttt gatgaagtta
    16201 ttgtaccttc agtaaataat aaaacatatt atagtgatta tgaccgcagt ttgttatata
    16261 ttgcttgtac acgagctatg cataaactta agttgacata tactggtgaa ttgactcaac
    16321 ttatagatat gtagattata tacttattat gtaagtgttt acttatataa atataacctg
    16381 tcaataaaat tcattaataa atcaataaaa acaatatttg taaaagtaaa agctataaat
    16441 tatatattaa attttataat ttatagcttt tataatactg cttatcttaa tcctcccaat
    16501 agcaaaataa ttttaaacat gccatatttt ataaatgtaa taacaattat acaattaaaa
    16561 taactttccc aaagtaactt gacatttaaa ac
//
LOCUS       DAEOSP010000072        16424 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.072, whole genome shotgun sequence.
ACCESSION   DAEOSP010000072 DAEOSP010000000
VERSION     DAEOSP010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16424
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.072"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            109..1473
                     /locus_tag="KRH54_001891"
     CDS             109..1473
                     /locus_tag="KRH54_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBG8579549.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMISAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            1612..2778
                     /locus_tag="KRH54_001892"
     CDS             1612..2778
                     /locus_tag="KRH54_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG8579550.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            2895..3677
                     /locus_tag="KRH54_001893"
     CDS             2895..3677
                     /locus_tag="KRH54_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBG8579551.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3796..4449)
                     /locus_tag="KRH54_001894"
     CDS             complement(3796..4449)
                     /locus_tag="KRH54_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8579552.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4473..5837)
                     /locus_tag="KRH54_001895"
     CDS             complement(4473..5837)
                     /locus_tag="KRH54_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG8579553.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
     gene            6495..7925
                     /locus_tag="KRH54_001896"
     CDS             6495..7925
                     /locus_tag="KRH54_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8579554.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            8028..9131
                     /locus_tag="KRH54_001897"
     CDS             8028..9131
                     /locus_tag="KRH54_001897"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG8579555.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENRKFVAVLKEILNIKGEPSIG"
     gene            9146..9382
                     /locus_tag="KRH54_001898"
     CDS             9146..9382
                     /locus_tag="KRH54_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG8579556.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            9399..10448
                     /gene="vorB"
                     /locus_tag="KRH54_001899"
     CDS             9399..10448
                     /gene="vorB"
                     /locus_tag="KRH54_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG8579557.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            10450..11190
                     /locus_tag="KRH54_001900"
     CDS             10450..11190
                     /locus_tag="KRH54_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG8579558.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            11193..11732
                     /locus_tag="KRH54_001901"
     CDS             11193..11732
                     /locus_tag="KRH54_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG8579559.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            11856..13955
                     /locus_tag="KRH54_001902"
     CDS             11856..13955
                     /locus_tag="KRH54_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBG8579560.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLYTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPLLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(14222..14737)
                     /locus_tag="KRH54_001903"
     CDS             complement(14222..14737)
                     /locus_tag="KRH54_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBG8579561.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(14829..16040)
                     /locus_tag="KRH54_001904"
     CDS             complement(14829..16040)
                     /locus_tag="KRH54_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBG8579562.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNISNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         6217 a         2013 c         2796 g         5398 t
ORIGIN      
        1 tgttatattg agatgaattt attatgatat aaaaaataat gttaggataa atttaaatct
       61 ttttctaaaa atgtattaat ataattctta aagaaaggat ttttaataat ggatataaaa
      121 gagttagtta aaatgcaaag aaaatattat aatacaggta aaacaagaga tatatcgttt
      181 cgtatagaac agttgaagaa acttaagtta gttgtatcac aaaatgaaga aaaaatactt
      241 ttagcactaa agaaagattt aaataaatcg gattttgaag gttttatgac agaagtaggc
      301 atgttttata gtgaattaaa ctttgctata aagaatataa gaaaatggag caaaataaaa
      361 agagttaaat caagtatggt taattttcca tctatatcca aagttgttcc acaaccttat
      421 ggtgttactc tcataatgtc accttggaat tatccatttc aattagcact tataccatta
      481 gtttggtcat tagcagcagg gaattgtgtt atattaaaac cttctgaata ttctgtgtca
      541 acatctagtg tagttaaaga catagtggaa gatacttttt caaaagaata tgttgcagtt
      601 gttcaaggaa gtcaagaaga aagtgaaaag ttactattgg aaagatttga ttatatattt
      661 tttacaggaa gtactaatgt tggtaaaata atcatgaaat cagcatcaga gcatttaact
      721 cctattactt tagaacttgg aggaaaaagt ccttgtatca ttttaaaaga tgctaatata
      781 gaccttactg ccaaaagact tacttgggga aaactaataa atgcaggaca aacctgcgtg
      841 gctccagatt atgttttagt gcatgaagat agaaaaaatg agttaataga gaaaataaaa
      901 tattatacta ataagtattt tggagataat ccatgtaata atgagcaatt tcctaaaatc
      961 ataaatcaga gacattttaa tcgaatatta tctcttattg ataaagataa gatagtctat
     1021 ggtggaaatt ataataagga gacattaaag atagagccaa ctatagtaga caatgttaat
     1081 tgggatgata atataatgaa ggaagaaata tttgggccta tatttcccat tttaacatat
     1141 aaagatttgg atgaagtaat acaaaaaatt atacaaatgc caaatccatt ggcactttat
     1201 atttttacaa aaaataagta cctagaaaat aaacttttag aaatgatttc agcaggagga
     1261 tgttgtatca atgatacagt tactcatata gctacgaatt acttaccttt tggtggtatt
     1321 ggtgaaagtg gtatgggctc atatcatgga aaagctggat tcgatacatt tactcactat
     1381 aagagtgtgt taaaaaaatt aaatttggat gtccctatta gatatgctcc atacgataat
     1441 aaattaatta aggttcttaa aaagataatg taggttatat tgtagaataa taaatataaa
     1501 taaaattgag tatttgtatt ctgaatatat tttattttta atttggtata tttgttataa
     1561 aagtggtgat tttgtgataa aatttaatta tatagaaata aggaggaaaa aatgaaaatt
     1621 aattggaata caaaatacac aacaatagca atttatacat ttataatagc tgctagtagt
     1681 attatatttt atctagtatc atcacaaatt gatgtatttt ctaataattt agatgctata
     1741 tttacaacat tacaaccttt tataatagga tttgctattg catatcttct taattttatt
     1801 ttaaagtttt atgaagacag aatttttata aaaagtgaga aattaaaaaa actaaaacaa
     1861 agttcaaaaa gaggcttggg tcttttatta acatatgcaa cagctacatt gatactttac
     1921 ttatttatgc atttcgtatt gccacaagta attgagagta ttgttggact agcaaatgat
     1981 atacctatgt atgtaaacaa tgcaactaag cttattgata aattaatgac tgatttaaat
     2041 cttgatgagc aatactttaa tttagcagtt gataagtgga atgagtttgt tacatatatt
     2101 ataaagtttg taacagattt aatcccaata ttaggaagta tgttaaagaa tgtagcctct
     2161 agtatatgga atattgtact aggattaatt gtttcagtat acttattaat tgataaagaa
     2221 aaattctatg gattaagcaa aaaaataact tatgcagtat ttacagaaaa acaagctgct
     2281 agaattttag aattaactca tagaagtaac tatacatttg gaagattttt aggtggcaaa
     2341 atattagatt cattcattat aggtattcta acatttgtaa tacttacatt agttaaaatg
     2401 ccatatacat tattaatatc agttataatt ggtattacaa atattatccc tttctttgga
     2461 cctttatttg gagctatacc atccacgcta atagtgatgt ttgtatctcc tataaaagct
     2521 ttctggttat tgttaattat attaataata caacagatag atggaaacat tataggacct
     2581 aaaatacttg gagattcaat tggtatatct gcattttgga ttttgttctc cttattggtt
     2641 gcaggaaaat tattaggttt tattggaatg gttataggag ttccaatgtt tgcagtaatt
     2701 tattctataa ttaaggatat tgttgaaagt aaattggaca aaaagggatt accgacagat
     2761 acttccgatt atatgtagct aaaaaatatt atgtataatt aaaaatatat aaattaagca
     2821 atatttaaag cactgagaaa gttaaactct tggtgctttt tagatataaa attattatag
     2881 aagaggtgtt ttttgtggca tttatagaat gtaaatttaa atctaaatat actggaggag
     2941 aaactgatat aattgtaatt ttgcctgtag caaatggaaa agaacttttt agtggtaaag
     3001 agatatatga ttttgaaggt aaatttaaaa cattatactt atttcatggt ttagaagatg
     3061 accaaagctc atggattaga tatactaatg tagagcatta tgctaaaaat aaaaatatag
     3121 ccctagtaat accaagagta gaaactagtt tttataccga tatggcgtat ggacaccaat
     3181 attttacttt tgtaagcgag gaattaccta aatttgttag agctatattt cctttatcag
     3241 ataaaaggga agataacttt gtagctggta tgtctatggg gggatatgga gctttaaaat
     3301 tagcgctttc aaaaccaaaa gagttttcgg cagttgcaag tttttctggt tcgcctgata
     3361 tgattcatga gttagaaaat caacaaatag aaaccaatgc agacattcta tttcacgatt
     3421 ttggtagacc agaagacgct agaaatagtg agaatgattt actttacata cttaagaacc
     3481 ttaaagaaag aaatgaagat attcctaaaa tttatcaatt ttgtggtgat agagattttt
     3541 tatataaaaa taatcagatg tttaaaaaat atgcagattc tctgggtgta gatgttgaat
     3601 atgaagagtg tgtagggggg catgaatggc gtctttggaa tttatggata aagaaattta
     3661 tagatataat tggatagtac acttatggta ttaacaggta tactataaaa tatctttaaa
     3721 tatgataaag actgaaatcc taaaatcaaa ttatgatttt aaaatttcag tctctaagaa
     3781 tttaataaaa ttttattaag ttcttacacg agttattatt ggagtaaaat tgtcaactat
     3841 atctatatag tatttactca attcctctaa aatctcttcg cataaatccg tactatgacc
     3901 aagattaata agaaactgtt ttgtaaaatg ctttattaca gcttgagaag atatatcgta
     3961 cttgtgtgaa gtataaactc taattatttc aggtttcata gcaagtatac caatacaaga
     4021 aagtattaat tcatcatgtt ctatagattc tttatcatta acatatcttt tcatgaccat
     4081 ttcggttaca cttctttgat agcttaaaat agcttcttca taagctatat ccaaataaaa
     4141 atttctatat gcttcgtttt ttagaaggaa acaaaggtca gcattcatag caagaaagaa
     4201 catcatcata gtaccttctt catggtagtt atagattaaa gactgcattt catttcttat
     4261 atcttcatat attatagctc ctatttcgct tttacttgag aagtaatagc ttattagacc
     4321 taaatttata ttagctcttt ctgcaatttg cctagttgtt gtcttatgat aaccttgctc
     4381 aaaaaataat tctctagcag cacttataat actagctttt gtttctttgc ccttttctga
     4441 ttttgacata tttactgtgt atacctctct tttcaatctt tttttgaaaa tttgctaact
     4501 ataaatattg taatagaacc acctattata ggaactaaaa ttctgtcaaa tacagtaaga
     4561 ataccaatca tatttaataa tgccaatata acacttacta taacagcata gatagctcct
     4621 ttttttgtag cacctttcca gcaaagacca attacaaatg gtgcaaatat tacacctgta
     4681 cataatggag ctggccaaaa caataattct aataaactat caaatttaag tgctatgatt
     4741 gcacctatta tagctgtcaa agctgatgca atttgagaat actttagagt attttttaaa
     4801 tcattaaatt ctatattagg atgtaataat ttgttataaa tatctcttga tataatagca
     4861 ctcattgagg tcattaatga atcggcagta gacattatgg cagccaatat agcagctaag
     4921 aataatacag atattaaagg tggcaaagta gtcgatatca tttgaggtaa aacctgaaca
     4981 gatgatatat ttggaaataa actaccacca atagctccaa ttattcctat agggaaagca
     5041 agaaataata gtataaatcc tgcaattaca ctacttttaa ctgcattttt agcattatca
     5101 gcacattgat atcttaaaaa actttcataa gaaaatattg agcctaaaac taatggtaaa
     5161 actgtataca taaaatcact aatcgattta tttcctggaa agaatggttc ttttaccatt
     5221 gattgtaaca taagtaaagg gttgtcgcca actaatttta agcttatata agctactatt
     5281 ggcaaaaaca ctatacaaat agcaagttga ataaatgctg atactgcaac tccccacatt
     5341 cctgatgtat aggtatatat aattataact ataactccta taattacagc tatatcataa
     5401 ggaataccaa atgcagtaga tacagaacca aaagaaatta attgactacc cataatagct
     5461 agtatggata ttggtgttac aagactttga taagctctta aaaatacact atgaccatat
     5521 ctagtttcaa tataatcagc aggtgttata tcacaagata agcttcttaa ttttcctgcg
     5581 aaaaatgcat ttataaagca agctaaagct gcacctatag tagaatacat tccaacccaa
     5641 agtcctcttt cagctcccca ttgaactcca ccaactaagc accctcctcc aaaagatgta
     5701 gcagcaatgg ttgcagctag tataggcaaa ggcagatttc tacctgcgat taggaaatcg
     5761 gatgctgttt taactttttt tgaaaaataa aaaccaatcc atatgtatat tgcacacgta
     5821 acaattaaaa gtaacatagt cttttctcct ttataaaata ttttaataaa atatacaaaa
     5881 tatatttaaa atatatttta tataaaagta gtacgatatt tgttttattg gttttagatt
     5941 aatatagcta taataattta tgatattaga tggaaattac gtaaaaatag aaaattgaaa
     6001 attctgaaat ttttgcatat tgatatttat ctcatagatt cttttttgta attgtaacac
     6061 gcttttattt ttaaatcaat agtacatgta taaaaataaa aggaatttaa ataagaaaaa
     6121 gatattttta atatctggat gtagtataga aatatcttga ataaattatt cttgaataaa
     6181 attataaaaa attaaaatca ctagaaatat gataaaataa tttaaaatac gcatttcaat
     6241 gacatttcaa aacaagaaaa taataaaaaa caaacctaaa ttaagtctaa atagcttttt
     6301 agttaatttt agtagtaaaa aataaaactt aaaaagtatt tgacaattat gaattttgag
     6361 aatataatta aattaaaatt atacacatat aaaaatatat tcctgaatca agaatgagat
     6421 atttaagatt caaaatatat attattatac gtgaataata aaaagtaaga aaattttata
     6481 agggaggggt agatgtgagt agtcaagaaa gcaaaccaaa aggaaatctt atatcaaatt
     6541 ttagagatga tatgagagac cctaagaaaa gagcaatata cacatattca agttctataa
     6601 acttagcgta caattttata ggttactttg tatcaactgt tacaaatatt tttttaactg
     6661 atgtggcaaa acttggtgta attatggcag gtggtatgca aattatacaa tctttcataa
     6721 aaattgctgt agctcctata acaggtacag tatttgacaa acaaccattc aaaaaaggaa
     6781 agtattatcc atggcttaga tatgcaccag cagcgttagc attaacatat ggaatatttt
     6841 tcatagttcc attaatgaaa ataagtccag cattactttt acctttgatg acagctttat
     6901 tattaatagc atcttgtgct cagcaagtag ttttcactat aatttatgct atttaccctg
     6961 tagttgctaa gacacctaga gaccgagtta taggctcaac tactacgaat atatttaaag
     7021 aagttggtaa attcttagta ggtttttcat atccgttatt attagtttta tttgcagatg
     7081 tattaggtgg agaaggaatg ggttatttag gtacctactt attctttgca gcaggatgcc
     7141 ttttagtatt ttggttttct tcaacagaga taataaaatc tggagcagaa gaagaagttc
     7201 ttcaaaaaga agagaagcca aagattaaag caagtgaaat ggcaaaagct ttattcacta
     7261 atgtaccact tatgatggca ttttgtttag aatttcttat atgtataaga tctataggat
     7321 taggaccttt ggctccatat tattttaaat atgtaataga agatgaacgt ggattagcag
     7381 tatttttatc aatcatgcct ttggtaagtg tagcattcat gtttttcgca ccagtgttta
     7441 taaaaatctg tagagagcaa aaactagctt caataatttc tttttctata tgtgcactat
     7501 gccacttatt ggttgcatgg actccttggg ggaaaactac tttaggagta actatgttgc
     7561 tggcaattgg tggaggtttt tcaaatgtag tatcaataat aaatcttaat ttctttgccg
     7621 gttcttgtga ttatgggcat tggaaatcag gaaaagattt gcctggttta agtatgtcac
     7681 tatatccagt tgcaatacaa gtaggagtat tacttgcaac tactataaga acagttttaa
     7741 tgaactcaat gggatatcaa gctgatatgg tagtcacaga agcagttaaa tcaggtttta
     7801 taaatatgat ttcttattct atggcaatac ctttaataat agctgttgtt atagcaatac
     7861 tatatccagt aagtgacaag aaattaaacc aaataagaga agagttaaat caaagaaatg
     7921 cttagttaaa agaaagttaa gtagatggtt aatttaagct attaaagtaa gtagacgcaa
     7981 tagcgtttat ataaatgaaa atacatatac agagggggct tttaaaaatg atagaaaaat
     8041 tattgagaaa taccgtaaaa gaattacatc aatatgttcc aggtgaacca atagaaaaag
     8101 ttaaggaaaa atatggtgta aaagaaataa taaaattagc ttcaaatgaa aatcctctag
     8161 gaccatcacc aaaagcaata gaagctatga ttgaaatgct aaaacaaggt caactttatc
     8221 cagagccaga agctaacgaa cttagaagaa aattagctga aaagctagat ttaaaaccag
     8281 aaaattttat tgttgctaat ggtgcggata acgttatcac tctaataggt gaggctttta
     8341 taaatagagg agatgaagtt atatattgta acccaacatt tccatcatat agaactgcaa
     8401 ctataaaaaa tgaagggata ccagtagaag tacctttaac agaagattat aagtatgatt
     8461 tacaaggtat attagataag attacagata aaacaaaatt aatatgtgta tgtaacccta
     8521 ataatccaac aggaacaatt gtggatgata aagagttaga agaattttta aagaaagtcc
     8581 ctgaaaatat tataactata ttggatgaag catatataga atttttaaca gttcctaatt
     8641 atgtggatgg acttaagtat gtaagggaaa attataacgt tatagtaaca agaactttct
     8701 ctaaaatata tggtcttgca gggcttagag taggatatgg tattgctaaa gatgagataa
     8761 taagaacttt atttacagta aaagagcctt tctctgcaaa tcgtgttgct ataagtggag
     8821 caacagcagc acttgatgat gaagagttta taaaagaaac atatgaatta aatagagttg
     8881 gaatggctta ctttaaagaa gagtttacaa aaatgggatt tgatgttgta gattcacaat
     8941 caaatttcct ttatgtagat atgaaaacag atatacctaa gttatttgaa gacttgaaaa
     9001 aaagagggtt tgtaataaga ccaagtgcta cacatgcacg tgtaagtatt ggaactatgg
     9061 aagaaaatag gaaatttgta gcagttttaa aggaaatatt aaatattaaa ggagagccaa
     9121 gtattggata atagatagaa atattatgaa acggggtgta gatatgaact taaaaataaa
     9181 cgcagagaga tgtaaaagtt gtgaatactg cgttatatct tgtaaaaaag gagcattaaa
     9241 aatatcaagt aagataaata aagaaggtta tgctcatgta gaagttgatg agagtaaatg
     9301 catactttgt ggaatttgct atcaggtgtg tccagataat gtttttgaaa taattaaaga
     9361 agaagcatgt caagaggctt agaaggagtt gggataatat gaaaaagatg tggaagggga
     9421 accacgccat tgcagaagca gccctaagag gaggttgtga attttatgca ggatatccaa
     9481 ttacaccaca aacagaggta atggaatttt tatcacatag aatgtctgaa cttggaagaa
     9541 cttttataca gtccgaaaat gagatggctg ctatatatat ggtatatgga gcatatgcaa
     9601 gtggaatgag gtctatgacg agttcttcag gaccaggaat atctttaaaa caagaaggga
     9661 tttcttactt atgtgcaaat cattaccctt gtgtaatcgt aaatgtacag agatgggggc
     9721 ctggtcttgg ctcattggac tcagcacaga cagactatct aagagatact agagggggag
     9781 gaaatggaga ttatcatttg atagtttatg caccaaacag tattcaagag actgtagatt
     9841 tgatgtataa ctcatttgat gtagcggaga agtacagagt gcctgtcgaa attttgacag
     9901 aagcagcttt aggtcaaatg atggaaccag tagaatttcc agagtttaag aagagagaag
     9961 gagatttagg ttggacttat gatggaagta atagagacca tgcaaaggtt gcagataacc
    10021 aaaagccaac tttttgtatg gagaaacgta tgagaataac tgaaaatgaa caacaatggg
    10081 aagattatca agtagaagat gcagaatatg tgtttgtagc gtttggtata ccatcaagaa
    10141 caactatgaa tgctgttaga agacttagag aacaaggtga aaaagttgga ataataagac
    10201 caataacagt atggcctttc ccatataaag catttgaaaa ggtaagtaaa gatgtaaaag
    10261 gatttatctc agtagaatca acagacacag gtcaattagt agaagatgtt gctttagcct
    10321 caaagaaagt atgtaaagaa aatgtacctg tgtatggttt atttagtgga aatcatatac
    10381 caaaaacatt gcaagtaatg gacacttata gcaaaataaa atctggcgaa atcaaggagg
    10441 tgttctaata tggctacaga aaaattaaca cctagcgttg tttcaatacc aaacaagttt
    10501 tgcccaggtt gtggtcatgg aatagtaaat cgtataatag cagaggtaat agaagaacat
    10561 ggatatgaaa aaaatcatgt attaacatta ggagttggtt gtgtctgcaa tatgaatttt
    10621 agctggaatg gtgataagat gcaaactgca catggtagag cttcatctac agctatagga
    10681 gttaaagtag cacttccaga tacattagta atgacttacc aaggtgatgg agatgcttat
    10741 gttataggtc tttctgaaac tttaaataca gcatatagaa atcataatgt gactgtattt
    10801 gtaataaaca ataataattt tgcaatgact ggtggacaaa tgtcatggac cactatgccg
    10861 ggtcaagtca caacaacaag tgtaaatgga agagatataa agactactgg tagtccactg
    10921 aaagttcctg agatggtggc aaacttcttt gatgtagcat atgtagcaag aggttcagtt
    10981 catagcccaa aggaaataat taatctaaaa aaatatataa aaaatgcaat agaagctcaa
    11041 ttaaatggag agggatattc cttagttgaa atattagcac cttgcccaac taactggggt
    11101 gtatcactag agaaatctat caaatggatg gaagaagaaa tagtgccata ttatgctctt
    11161 ggcgaattta agcaaaggga tggtgaatag ttatggaaaa agagctagta tttgcagggt
    11221 ttggtggtca aggagtttta actcttggtc tgattgtagc tgagtcagca cttgaattgg
    11281 ggaaacaagt aacttggatg cctgcatatg gtccaactat gagaggtgga aaagcctata
    11341 gtgtagtaaa gttttctgat gagagtatag gtgggcctga tatggaagaa atagatgttt
    11401 tagtagcaat gaataaacca tctttagatt atataaattt agttaaagaa ggtggaacag
    11461 tagttattaa tacatctgca atagatgaga atgtaacatt aagagaagat ataagtgtag
    11521 ttaaaataaa ttgtcaggaa ttagctcaaa aagtaaacaa tccaaaagca gcaaatatag
    11581 tagtacttgg agcattaatt gctaaaactg ggttatttga aaaagaatta gctttaaaaa
    11641 ctatgtgtga tttctttgag gaaaaaggaa aaggaaagtt taatgttcaa aatgaagcag
    11701 cattcatgga aggctataat aacgccattt gataaattac ccttatctta agtgatttat
    11761 ctcaagattt aataatcttt agtgccaaga gaaacttcat cttgagataa aattcactta
    11821 aaatcacaac tataaacata taggagcgtg aatttatgag tttggaaaaa cttattagac
    11881 caaaaagtat tgctatagta ggagtgactg ataaacttgg ttttggaaga agtgcagcac
    11941 ttagtatagt aaaaagtaag gaaacagata gagtatatta tgttaatccc aaaagagaag
    12001 aattgtttgg gagaaaatgt tataagacga tacaagaagt tccagaagta gtagactgtg
    12061 tagtagtatg tacgcctaga aatgtagttc catctgtatt aaaagattct ggagagttag
    12121 gaacaaaggc agcagtggta tatgctagtg gatttgcaga ggaaggtaca gaggaaggta
    12181 cagatttaga aaatcaactt atagaaatat caaatacata cgatatgaaa atattgggtc
    12241 caaactgcat ggggttgcta aattgtatag ataaggttaa tctatgggca ggaggttcta
    12301 aatgggattt atatactaaa aaacctggta taggaattgt aggtcaaagt ggatttataa
    12361 ctgctgaaat tgtttcaagt gattatttta atatatctta tggtttttca acaggaaatg
    12421 gaaatatagt tacactagaa gatgcagtag atttccttgt agatgatgat tatgcatcag
    12481 taatagcaat ttaccttgaa ggtttaaaaa atcctcaaaa atttatagat gctttaaaaa
    12541 gggcagcaca aaaaagaaag ccagtaataa tattgaaatc aggaaggtca gaaaaaggtg
    12601 ctatttctgc tgcttctcat acaggaaatt tagctggttc gagtaaagct tttgaaagta
    12661 tttttgaaaa atatggagta atttctgtgg aaaatttaga acagtttatg tgtttagcac
    12721 aagcttttag tgttttagat ggtaatttac ctgcaaattc taattttgca gcaataaact
    12781 tctcaggagg agaaaatact atttgtgcag atttagcaga agaaaatggt gtagaacttg
    12841 cagaaatatc ttctgaaact aaagaagaaa tgaaaaaata tcttccagga tttgcaacac
    12901 ctaaaaatcc actagatgct acaacagcat tattccatga aaaggatatg atagtaggtt
    12961 tattacatac ctttgaaaag gatgatagtg ttggatttac tatggtaggt gcaaatataa
    13021 gagatgaaga aaatgaaatg catgaaaccc tttgtaaagc tgtaagtgaa gctagagaac
    13081 aaggattgaa aaaacctgtg tttgcagttc ctacacttga aggaaataga tatttagatt
    13141 ataggactag attagaagat aatcaagtgc ctataatgtc atcagttgga actacattta
    13201 cttgttttaa taaaatggct aagtttatag attatgatta ttcaaaaaga actttagaat
    13261 ttaaagctgt taagaaaaga gagtcaaata atgtagtagc attatcagaa ttagatagta
    13321 agattgagat gaaaaaatat ggagtaccag ttccaggaca aggtaatgca aaaagtattg
    13381 atgaattaga tgaaatctta aaatatatta agtatcctgt tgttttaaaa ataaattctt
    13441 ctgaaatatt gcacaaatct gatgttggag gagttaaaat aggaattaag aatagagatg
    13501 aggcagtaga tgcatataat gagattttaa ctaatgtaaa aaaagcaaaa ccagatgcta
    13561 atattgatgg tatattagtt caagaaatgg tagaaagtgg tatagaaatt attattggta
    13621 taactaatga tgaccaattt ggaccactgt tattagtcgg tttaggagga gtatttgtag
    13681 aggtatttaa agatactaca ttatatccat tacctataaa tcatgatgaa gctattatga
    13741 tgcttaaaaa actaaaatca tttaagcttt taaatggcta tagaggaagt gagccttgcg
    13801 atattgatgc tcttgcagat atgatggtca aattaggaaa atatgcttat gaaaataagg
    13861 atgaagtcaa ggaaatagac ttaaatccag tgtttgtata tcctaaagga aaaggcgtat
    13921 gtgcagtaga tgccttaata gttaaatata aatagtttat aagttttggt attcaaccat
    13981 aattaaagca ccgactgaaa atcacatttt gtaagttaga tagtgtgatt ttcagtcacc
    14041 ccaaatttaa tttaaaaata ttttaaaatg aaagtatagt tttaagtttc attgtgaaat
    14101 agcactgtat aaacaataaa atactacgta taaaacgaat aataaaaggt gttttaatga
    14161 taagaatcaa tcttgatttt cattaaaaca ccttctttat tatattaaaa atttttaaac
    14221 gttatattgt cacaggtggg tttgttggag ctgcatatgt acgattagca aattcttggt
    14281 caacaaagaa tagaccaccc tctccagcaa gtttaacacg ttttatattg tcttcacaat
    14341 ttgtctcttc ttctgcttgt tcagatataa accaaagtaa gaattgagaa gttctaaagt
    14401 ctgattcaga gtttgctaca gctgctaagt tgtttattaa agctgtaacc tttttttcat
    14461 gagctaacgt acgctcaaga atatccattg cagaagtaaa gtctgaatct ggcattggga
    14521 tagcatcaag ctgaaccttt cctcctacat tatgtaagta tttgtaaaaa aacatagcgt
    14581 ggtctgtctc ttctttatat tgaacataaa accaattaga aaatccttta agtccttctg
    14641 cttcaaggta agaagacata gaaagataaa gatacgcaga atataattca tggttaatct
    14701 gctcattaag taatttttcc attttagctg aaatcatttt tatacctcca aattttaaat
    14761 gctcttataa aatataataa tattaaatgg aaaaatattc aataaaagtg tttaaatatt
    14821 ttacatattt aataaaaatt tatactacca ttaaaaaaat tataacttac aatggctata
    14881 gagccaatta atggtgcatt tgctccaaaa tttgaatgcc ttacacttat ttttttatat
    14941 ttagaaaaac ttgctgaagc agtcaatttt tttaataatg tattttctat aaatgtacca
    15001 ggtatattaa agtcatagcc tattattata gaactacaat caatcaatgt aagtgtatta
    15061 actaatgcat atgatacgta tgtacaaaat tcatcaagca aaaacatggc aagactatct
    15121 ttattatttg cctcatctat taacttgact aaagaaaaat ctttacagtt aattaatggg
    15181 gagttaggat aaatatggct caatgattca attcttttaa ttagattact gacacttgta
    15241 taataatcta aacatccatt gttaccacaa ctgcacaatg gtccactaaa attaattgat
    15301 gtatgtccta tttctccact ttgtcctagg ttgccagtat gaagtttatt ttctagaact
    15361 agaccagctc caataccatt cattatgtgt aggtatatat aatttgatat atttttacct
    15421 aaaccataca ttttttctgc taatgcaccc gaattagcat catttatcaa aaatattggc
    15481 aaatcagaca tttcttttat aaaatctact atatttaggt ttgatatatt tccaaaatct
    15541 gggggattta gtatggtacc atttatatca tctactggac ctattgaaga aataccaatt
    15601 ccaattatat ctctcttgtt gattttcatt attttagtaa acattctttt tattatatct
    15661 tttaaaacat catttgatag acatttaggg taagtttcag aaagttgttt tacaatatta
    15721 ccttttaaat ctgaaataat taccttacat atatttcttc ttattaggat tccacaaata
    15781 caaggcgata tattagaaat atctaataaa atgggtttac gacctgatga atgtgcattt
    15841 aaagttgatg actctatttc cctaatcata ttttcattaa taagttcagt aactagattt
    15901 gataaagtca ttttagtaag tcctgtagac ttagctaaat caactctgga cataggtgta
    15961 tttgtagaaa gtaatttcaa tattattaac ttatttttaa tttttacatc tttgatgtta
    16021 gaaccttgtt tgttactcat cataaatctc cttaaataaa gttaagtgtt ttatttattt
    16081 tcaagtatac tatcacctta taatagagta acattttttt acttgaaaat atcattaatt
    16141 agaatatatc acattatata tcataaaaca tataaaaaaa tattagaatt aaattaaaag
    16201 tgaaaagtag attgatatat aaatagtaaa tacttaatta aaaaaagata atgatagcaa
    16261 taattattgt taccaagaat ctactacttc aagtgttacc ttaggtattt aagttgatag
    16321 tggtttgcac gtaataaact tgatgtaata tatattctta ataatattga taaaagattt
    16381 acttaaaaat aatatagatc atgtaaacat ataatctatt tatt
//
LOCUS       DAEOSP010000073        16422 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.073, whole genome shotgun sequence.
ACCESSION   DAEOSP010000073 DAEOSP010000000
VERSION     DAEOSP010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16422
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.073"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            142..582
                     /locus_tag="KRH54_001905"
     CDS             142..582
                     /locus_tag="KRH54_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG8579563.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(767..955)
                     /locus_tag="KRH54_001906"
     CDS             complement(767..955)
                     /locus_tag="KRH54_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579564.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
     gene            1896..2291
                     /locus_tag="KRH54_001907"
     CDS             1896..2291
                     /locus_tag="KRH54_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579565.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            2334..4433
                     /locus_tag="KRH54_001908"
     CDS             2334..4433
                     /locus_tag="KRH54_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBG8579566.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGETMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(4658..9868)
                     /locus_tag="KRH54_001909"
     CDS             complement(4658..9868)
                     /locus_tag="KRH54_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBG8579567.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKALIGNVTEEKIRNILKKAKEDLDSQEKDYDGYKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLNEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNTLYEEHKDWTISKREDSGSA
                     GAEGKAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNTKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSSKEEEYKWTNRMVNFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNRKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(9883..12378)
                     /locus_tag="KRH54_001910"
     CDS             complement(9883..12378)
                     /locus_tag="KRH54_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8579568.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDERDGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFISIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIDR
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMDFASEKNIILACNNL
                     DVGSNVKINVSGKSGELVDSSMYGKNGEDGRDSGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGKNGETGASGSNGADAGYTSYFHGQMFKGYFAKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGSGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(12968..13255)
                     /locus_tag="KRH54_001911"
     CDS             complement(12968..13255)
                     /locus_tag="KRH54_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579569.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(13275..13550)
                     /locus_tag="KRH54_001912"
     CDS             complement(13275..13550)
                     /locus_tag="KRH54_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579570.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(14183..14362)
                     /locus_tag="KRH54_001913"
     CDS             complement(14183..14362)
                     /locus_tag="KRH54_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579571.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(14647..16113)
                     /locus_tag="KRH54_001914"
     CDS             complement(14647..16113)
                     /locus_tag="KRH54_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8579572.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLLTYM
                     YKACRESILKIVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIGDCL
                     PSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDDYINTEEGAK
                     RLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDFVLL
                     CKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYLKKG
                     DVERFAESFSNIFKESLQYLKKVISSLNVDMRVKLDIL"
BASE COUNT         5460 a         2862 c         1840 g         6260 t
ORIGIN      
        1 ctattagcta aaactaatta ccttatattt ttacgtattg gtataataat acataaaaac
       61 ttgaataatg cataaaaact tgacatactg ttgtcataat tactttgcta taattaaaag
      121 taaaaatgga ggtaaattct aatgtatgaa cgtatgcttg ataaaagcaa taaaccctgt
      181 ttgtctcaaa taatagaata tataggtaaa gatggatatg cacgattgtg taagttggaa
      241 acttacttga aaactcaata taatttatca agagaacttc gatttccttt tggaaacaat
      301 tatggttggg gttacaaata tagccataaa tcttttcatt tattttatgc tttttttgaa
      361 aaaggtgcat ttactgttct gattcagata ggtgacaagc aagttccagc agtagaaaaa
      421 acgttggtag ctttgacacc aaaagctaaa gaactctggg tcactcgtta tccttgtggc
      481 aataatggag gctggattaa gtatcgtatt ttagaagatg cagacttact tgatattatt
      541 caatatataa gagcaaaaaa atctccacct aaacaaaatt aacttttatt ttttgtgatt
      601 taaatatagt gtatataaat tccattcttt ccatataaaa aaaataatat aaaaaatatc
      661 tattagtata acttatttag tatgtcttat tctacttttt acagataatt aaaaagtttt
      721 atcataaata aatcataact agacatccta gtaaatcaaa attaatttat tcttttttat
      781 tagaattttt gataacagtc cctatacata tgccaattat cattccaaaa attagaccat
      841 aacacattgt gatacctaca tttcctttag aaatacctat agcacaacct atagccattc
      901 caagtatcat gcctataaac atacatataa taactaaatt gttgttttta ttcatagttt
      961 ttctccattc aatatatttt aaattgccta gctcgccttc ttagcttaaa acaaagaaat
     1021 ataaattaac tttctttcat tctaatatct gcttgtataa atgtcaaaca acacatttct
     1081 tgtcataacc tcctcttttt gaagtaagta caatctccca gacgcaataa tatcaatttc
     1141 ctaaaaccat ttaagtaact tttatattta cataatattt acaatcataa actatattgt
     1201 tattcattta tacatcataa acactccctc ctaatcatta ttatacttaa aataatgttc
     1261 atttataata atatatctaa cgcactttaa ttgaaaaaac taaaactttg cctttcattc
     1321 ccatatataa ataagtatac tttttgatgc tctattacaa atcaactaaa ttaattgata
     1381 taaattatca ttttctgttg aaaataccat tcgaacatgc tatacttaat tgcataaatg
     1441 gtaataaatg tcatatgatt ttacaaatct attaataaca attattactt ttactgtatt
     1501 ctaaagtttt tggactaaat atcaagaact ttctaaaata caaagtacaa aaaatttata
     1561 taagttataa atttatatga actagataag ctcaaacaat atgttatgta taaactgtgt
     1621 agtattcggt tataaattta tataaattat ataagctaga taaagtatta cctcaactat
     1681 ataagctaca tatataaatt acatacataa gctatataag aattatttca actatataaa
     1741 ctacgcatgt aaaattcatg taaatttact aatataaatc aaataaaatt actaaactct
     1801 ctaaactaat tttgtaaaac atactctatt tttaacattt gttaccttat acataagtac
     1861 aaacaaaatc taactaaatg aaaggataat gtaaaatgaa tattttaaaa atgaaaaaaa
     1921 cagggatatt tttaggagga gtactttttg gtactgtagg tgttaagata ctttcaagtg
     1981 attgtgttaa aaaaacttgc actcgtgcaa cagcaggagc attgaaagtt aaagattctg
     2041 ttcttgaaac aactacgaaa attcaagaaa atgtagaaga tattttagct gaagcaaaag
     2101 aaatcaacga gcaagaagac ttatctacat ctaaggtaaa cgttgtacaa gatactctta
     2161 aatgcgaaca agaagttctt aaaactgaaa ctataaaaac tgtagaagat actaattcta
     2221 atagcattaa atgtgaagaa atcaaagata acgtaattga agaaaaaaat agtacttgta
     2281 caacagaata aatttaatga ttattatgat taataatatc aggaggaagt aaattgaatt
     2341 tttatataaa acacgaaata agaggtcgta tacgctttga cttacaactt ggcaaattaa
     2401 acaacaagca gtccgatact ctcttatatt atttaacttc tataaatggt attaccaaag
     2461 caaaagttta cgatagaact ggtgatgcag tcatttatta cactggtgaa agagaataca
     2521 taataagaga aatcactaaa ttcagtttta acaatagcaa aatagaaaac ctagttcctg
     2581 aacataccag cagggaatta aacaattatt atagagacaa attagcaaat aaactgctag
     2641 tcaaaagtat ctccaaaata ttttttccaa agtccttaag atttttttta attggacttc
     2701 attctataaa atattttaaa aaaggtctct cttccctgct aaataaaaaa atagaagttt
     2761 ctgttttaga tgccactgct ataggtattt ctatttttag aaaagatatc aatacggctg
     2821 gttcagttat gtttcttctt ggtatcggtg aactattaga agaatggact agaaaaaaat
     2881 ctatagacga cttggctcaa agtatgtcac taaacattga aaaagtatgg ttaagaaaag
     2941 gtgacactga aatcttaatt ccaatttcag agataaagga aggagattta gtaagtgtaa
     3001 ctatgggaaa tatgattcca ttggatggag ttatagtatc aggagaaaca atggtcaatc
     3061 aggcatcact tacaggtgaa tcccttgcag ttaacaaaaa agaaggctct tacatatatg
     3121 ctggaactgt aatagagcaa ggcaatattg ttatgtgcgt caaggaaaaa gctggtacta
     3181 ctagattcca gaaaatagta actatgattg aggaatctga aaaactaaaa tcttctgtag
     3241 agagtaaatt tgaacatcta gcagatacat tagttccata cagcttttta ggaagtatat
     3301 taacctatgc aattactaga aaccctataa aatcactctc tatattaatg gtagatttct
     3361 cttgtgcatt aaaactctct atccctattt cagtactatc agccatgaga gaatgtaaca
     3421 ataacaacat tactgtaaaa ggtggaaaat ttcttgaagg tgttgcatgt gcagatacta
     3481 ttgtatttga taagacaggc acattaacaa aagctcaacc aactgtttca gatataatta
     3541 cattccaaaa ttataataag gaggatatgt taagacttgc agcttgctta gaagaacact
     3601 tcccacactc catagcaaat gcagtagttt atgaagctga aaaacaaggg cttagtcaca
     3661 aagaaatgca tacagaagta gaatatatta ttgctcatgg catttcaaca aaagttgatg
     3721 ataaaaaggt tgtaataggt agcaaacatt ttgtatttga agatgagaag tgtactatcc
     3781 cagacggtga ggaggataag tacaataatc tatcagatga gtattcacac ctatttatgg
     3841 caatatcagg taaattatca gcagttattt gcataaatga ccctttacga aaagaagcaa
     3901 aatatgtaat ttcaaattta agagaatgcg gaataaaaaa gattgtgatg atgactggag
     3961 atagcgaaaa aacagctaaa tctatagcaa gtaaagttgg tgttgatgaa tattattcag
     4021 aagtcttacc agaagacaaa gccaattttg ttaaaagtga aaaactcaaa ggtcgcaaag
     4081 taattatgat tggtgatgga atcaatgatt ctcctgctat ttcagaatca gatgtcggaa
     4141 ttgcaatgag tgaaggtgct gaaatagcaa gagagatttc agatattacc atatcagcag
     4201 ataatttgaa taatttaatc gttttaaagc agattagtaa taagcttatg aaaagagttg
     4261 atttaagttc taagtttatt atcggcttta atttaggttt gattttactt ggtgtaggtg
     4321 gatttgttag accaagtaca tctgcctttt tacacaacgc atctactgtc ggaataagtt
     4381 taaacagtat gacaaattta ttggaaaaca ccaatactta taactaccat taatctccat
     4441 tacataaaac atatgatata aaaaatttat aattcatctt tttaaaaaaa tatgtcttaa
     4501 aattagcttt tttctaatca aaattatata aagcatactt tacaaatttt aaactcaact
     4561 tatttctatt ttttacaaaa aaggagatga ttcaaatttt cattttgaaa catccccttt
     4621 tgtaaatttc tttaaaattt atatattaat tatattttca accaataatt gtattttcaa
     4681 ccaatctttc ttcatagcat tttaaatcat tttgctttag ataattaaat tcagactcta
     4741 cataaatacc acagcctacc aattcaattg tcaaattagg tatattttcc tttagtatac
     4801 tagtctgctc ataaataatt ctttccaata aatcaacttg taatatagaa aaatgcttca
     4861 taatctcccc atcactatct atatctttct tgatttcata ccaactcttt gacaaaattc
     4921 ccttcaaatc aatataccct agactcctca atatatctat actatcgctc catgcatact
     4981 tactaggttt atcaaagtat gactctagtt tcttcttctg ctcatttgta agtgaatcat
     5041 aagaaagctt cctattaaaa tgtgccttca aagaaatctt agccatagaa tatggacttc
     5101 taccagagtt taatcctgca tttttgaact tttcaattcc tttatcttct actgaagacc
     5161 atattgagtt tattgaatct gaatcattgt atttaacatc aagaccatgt gtaatattta
     5221 tatagttttg tggaaataca tattgataat agacatcttt tttatcatca tatagagaga
     5281 agtatccact atcatataaa tgaatatgca cctttacttt taaactatcc tctaaaatat
     5341 ttcgtctaag gttcgggata tttgcataca tggttacata ttttatggca tcaaagtttt
     5401 tatttaatgg aacttcaaaa ctaatttcat ctcctgtcaa aagctttttc acttcttctt
     5461 cattacaatc atatagagaa tatgtatttt caaatcccac acgtgatttc ccatctaaac
     5521 tgcctgttaa tgctaccttc aaaatatggt tatctgtttt ttgccattgg taagcatctt
     5581 ggtcaagaaa ctctctaatc tgtctatttt gctctttcgc ctgatttata acctcgtttt
     5641 catcttctgt aatccctaca tggcatctat ttaatatatt tactatattg cttccaaatg
     5701 ggaatgtcca tagttgaaaa gctgctgcct caacctgaca caagtatcta acctgattta
     5761 attcaatttg atggataagt tttttctgat attcattcat ctgattacca ctatctgaca
     5821 tttgataaat caattctccc attgcaagat tatctttttt cttatcaatc ttatcagtaa
     5881 tgataatttg agtttccatc atatcctgta cttttttaaa tattatagaa atctcctctg
     5941 aattttccat ttttaagctt ccaaacttcg tgcttaattc agttaaaact tccttcatct
     6001 ttaatttact tatctcattt tcaaaaatat cttgattttt aactgtacta tcgaattttt
     6061 ctacaaatgg tataacagat tctttcaaag ttttatcttt aaattccacc atttgagtat
     6121 tcaattcatc taatttctta atattatttt ctttatcttt aattgcctct ttaactacct
     6181 ctattttact ctcaaaacta gattttacat taagacaatt attaacacca ctaatcgcag
     6241 tatttataag agaacaagca gaacttccaa tactcaatgc atttttaatt ttagatgtct
     6301 tttgctgtct ttcaatagct tgtttacttt tctctagtgc agctaactta tctttttgct
     6361 gtttattatt gtaatttgcc tccaacatac ctatttcttt tttctggatt tttgtgtaaa
     6421 tttccttata ctcgctttca cttaacccct cttttgctct ttgaatatcg tcagctttgt
     6481 ctgcatagtt atcatcatct atagacttaa ctataatctc cttaatttgg tcaaatttag
     6541 ttcttctttc ttcatcaaca cctgccaaat tataagtcat tttatctaaa gcattttttc
     6601 tatcttcact tagctgttct ccaagtacaa atctcatacc atcagaatcc aatttaaaat
     6661 ctgaaaaatt aaccatcctg ttagtccatt tgtattcctc ttctttagat gaaagatttt
     6721 ccttcaaaga ttttgcactg tcaaatgccc ctagacaagc ttgaccaaca ccactaaatg
     6781 cagtcagtaa actcaatgcg ctttgtattc cagaaagaat taactgtgtt ttcatttgtt
     6841 cctcaagttt tgcctgtttc ttttttagtt cctctatgtc ttcttgttca tcttctttca
     6901 tctttataat ttcttgaatc aacaggttca tttttgcatt aacagaaagc attttattct
     6961 caagttcatt ttttaaaacc tctatttgtt gatttccaga ttcaatttga gttaggatac
     7021 tttccttata tgtattgaaa tatccttctt ttaactcatc tttcttccct tgttccaatt
     7081 ctcttaaggt cttatccaga taatctgtca aagaacgaat atctgtaaca cgatttcctc
     7141 caatttcact ttcttcaatc atctgcacat aaaatgctgt cttctgctca agtgcaaata
     7201 aaatcatact ccattcgcta aaattctgat tttttcctac aaaagattct attctatcag
     7261 actgcatcaa tttatttata aggtattcat agatttcctt tgcttcttta taaaattctt
     7321 tctttgtatt catatcatcc agagaaagta ctgctttatt tttcttctca aaactatagt
     7381 acttctgaag tatttctcta tttaattgca tctctatttt ctcaactgca tgaaaaattt
     7441 cataaatatg tgaaagttct aaaatatcag cttctaaaat tcctactgca cacaacagtc
     7501 taagatttct ataaccttta attccagaaa aaccaaatct ctcgaaaaat tcatagaaga
     7561 atgtatctat tgacaaagaa tactcctgct ttaacaattc cttttctttt agtgcatctc
     7621 cagagtgacc agcttttccc tctgctccgg ctgaacctga atcttcacgt ttgctaattg
     7681 tccagtcctt atgttcttca taaagagtat ttaatttctc tccatcatct gctgcttgct
     7741 tctgatttaa agcatgatta aacacatatc tatcaaaata atagtagttt ctatcactat
     7801 tatccattgg aggagaggta cctatatatc ttattgtttc ttcatatata cctctaattc
     7861 caccaagacc tcctgcacca ccagctcctc cagagcctcc ttttcctgct aaaccatgat
     7921 ttccatcttt tttacttata gcacttttta ataaaggagc ttttccaagg agataaacct
     7981 gtccagactt gcttccaaga cctgcatttc caccattacc tcctgtgcct ccagttccac
     8041 cttgttttcc tttttctgct tttacaacat agtattttgt acctttaaca ttagcaaatt
     8101 cttttgactc ttttttagaa cctattttag catttgcccc agcttctcca gttatccctg
     8161 tatctccttt ttttccatct tgtccatttc caccacgtcc accatctacg taaagtttaa
     8221 gttctcctct tttttctatt tctttaaaag ataatatgat atcacctgaa gtacctgcat
     8281 tattgccatt tcttccattc attccgtcgt aaatagaact ttctccaacc aatttttctg
     8341 catccttacc agatacgttg attgaataag tcccttctgg tatatgtaat ttttctgtaa
     8401 gtagtacaat attcttacct gagaagactt ctttctccat attacagtca attttcaact
     8461 cacaatctcc atacatatat atagatgaca aaatattaag ttgataacca gatattattt
     8521 tagtcaaatc cgataaattc actttaggta taggaatata tatagaaagt acattttctt
     8581 tctcatatga ttttatatat gtacaacttt gaatcttctc tatcctatac tctgcatctt
     8641 caagcttttt ggcttcttct tccctatatt cttcttctgc tatccaatat ttgatatctt
     8701 ttaatgtatc ttctaaagta ttttttagca atgattttaa caactcactt agaccaacaa
     8761 tatgagtctc actacagtaa ctatttaact ctaaaagaag acgtgtttct tctaatatat
     8821 tcactttctg tacgccatca ccatatgaaa gttcttgtac aaactccata agctctttca
     8881 tattattagt cctactacat aaatctgtat acatcttatc taattgaatc tttttatctt
     8941 ttagactatt tatagaagat agaatggaaa tagaatcttc catacctttt ttaacacttt
     9001 ctatataagg aactaaaagt ttttctaggt ctttataggt tacatcaaca gctttttgta
     9061 tttctatttt acaagaagca gtaagcatca atcccaaatc tgttttttca aatgtttcag
     9121 gtagattttt catttcattc aacaactttt ctggctgttt atctagtgga ttacataatt
     9181 taatagatac accatctgat aatgctacat ttaataagta cactaattcc ttataaccgt
     9241 cgtaatcttt ctcttgacta tctaggtctt cttttgcttt tttcaatata tttcttattt
     9301 tttcttctgt tacattaccg attagtgctt taggtatttt attgataaga aagaagaaat
     9361 tttctttata ctttgcataa tcaccaagca tatctttcaa atttttgcct gtttctatta
     9421 ataatgtatg tctagagtcc agtaaggatt gatagtctaa aataactacc atccctttca
     9481 cctttgaaga tgtagaaata gtttccctta caagcatagc accagcaatc atatactctg
     9541 ctccacggtt atcaaaaaaa cctgggcaat cacataatga aaaatctcta tttgcaatat
     9601 ctacaacttc tggacataaa gtcttagaat atacaccatt tccaatttcg acagctgaat
     9661 cttttgcagt tatatatatt tgtccagtct cttcatccgt tttttcttcc atcacacagc
     9721 cttttaaata atttatacaa gtactctttc caacgcctga agcacctagg aataacaaaa
     9781 ttgacttttc ttttaagtca tattttctaa gctcgtctct tccttcttcc aaaaggttta
     9841 cgatttcact ataaagttta tttgccatac atccatccct ttctatattt cggaacaata
     9901 gccttgatat ttttctttaa taagattctc ttctaccttt gaaacagctg ttgataactt
     9961 agtgtttgga gcattaaatc ctccattgtc tcctctatct ccatattcag cgtattctct
    10021 tgctggtcct gttttacctt cattccaact tccaccacta aattctatat gatatttaat
    10081 tatactatcc aacttataat cccaacttgt aaaatatcct gtatagtaca cagtcttatc
    10141 tataccactt ggagaaaatg aaagtgtatt gtatccgtga tagccaccac ttcctccata
    10201 tccacctgaa cctggtctac catttggacc tgcttctcca tctttcactg ttatatcaca
    10261 atgattaatt attctatttt tttgttcctc tgaatctgaa atgaagtaaa tattaccttt
    10321 cttaccagca tttccacctc ttccagcatt tccaccagct ccaccttttt cacctttttt
    10381 gccacttgta ccttttgcaa aatacccttt aaacatctgt ccgtggaaat aacttgtata
    10441 ccctgcatca gctccattac ttccagaagc tcctgtttca ccatttttcc catctattcc
    10501 tttagaacca tttcctccat ttgcaatcag tgttaaacga taatttctaa tttcttcttt
    10561 aatatataag taaatattac ctgaatctct accatcttca ccatttttac catacataga
    10621 actatctaca agttctccgc tctttccact tacattaatt tttacattac tacctacatc
    10681 aagattgtta caagcaagaa taatattttt ctctgatgca aagtccattc caaggttatc
    10741 atccatataa atagtatctt tagcaaagaa cataaatgta gaaacattgt catctacttt
    10801 tctatttttg aaatattcca atacactgct aattactaag tttggaatta atgctcttat
    10861 ttcaatacct ttcttatcat taatattaat tgtttttact ataaatggat ttaaaatgta
    10921 tttaccaata aattctctaa ttatctcatt accttcttct ccatatgcac ttaatcttgt
    10981 tatactgtct tctaactctt cattttgtgt aactgtaaca tttgtcaaaa ttgaattaaa
    11041 atcacttaca ctatatacat aatccttttt atttgcaact tccattaatt tttctctatt
    11101 tggtaatgta aatacctgtt tttgcacttc ataagaaact aaaacttttt ctaaattctt
    11161 tcttattata atgaagtttt gttccattat cttcttactt gccttttcta taataaattc
    11221 catatgcaca gaaggtaata aaatttcccc agttccatgt tctgtatatt tacttaaagc
    11281 atcaattata tctaagtttc cttttatctt attttgtaat ttcatgcaac tatcttgtaa
    11341 gaataagcca ctttgtttgg ctaatacgtt gataaatctg tctatagtaa gacttgttgt
    11401 actttccaac cagtttttaa atacttttac aacctcatca tattttgcta tttcttgaat
    11461 cattgttata cttccaataa tactgataaa atcttctgta tacgccttaa cataatcttc
    11521 taggctagat acgatattgt tttgaatttc ttttatcaac gtatctaaaa ctaatcttgc
    11581 atctgcacta attggatact ggaatgctga tgtcttatct ttaacttcat ctaattctag
    11641 tacttgcttt aataaaccgt ccctctcatc tgatgcaagt ggcttacaaa gatgaatatt
    11701 ttttccatta tcttttagca attcttcaaa taaccaagct ttttcctcta tattactgtc
    11761 taaattttcg cataattgtt catattttct ttttataaca ttatatattt gtttttctgt
    11821 tcttcctaag aaactttttg aaataaaaaa agttatacta cccttaaaat tttctttatg
    11881 aagacacata tttaactgtt ggaacaggtc tagtactttt gtaagtctag aatctaatat
    11941 atctgcttct ggtatcaata caattactgc acatatagat gcagaatttt taaatacttc
    12001 acctaatgaa actgctgcgc taatatcatg ggctactccc ctattgtcgc taaaacctgc
    12061 agtatcacat aattttgcat ccattccttc tattggtttt ctcatattta caatgttcgg
    12121 gtataaagtt tgagaaacag ctcctgaacc aatctctgcc acagggtcta agcaatcaat
    12181 atatgatata ccagtatctt catcttcgac ttccatcatt acacatcctg caagatagtt
    12241 aattgcagta cttttgccac atcctgtccc accaagaatt agaattaaat tttgattttc
    12301 agccttattt attgctactt ttccttcatt taataagagt gttatttctt ctaaaagttg
    12361 attgttattt tctggcattt attttccccc ttggttatat attttattta aataaagttt
    12421 aatcccttaa gtagattaca aagtttttag actatattta aatttgtttt ttatttttta
    12481 tttttaataa atttgcattt tcactaaatt tgtattttca ctcaataatt attcttcttt
    12541 gtttattaaa ataactgtgt tgtataatat aatctttacc aacacagtta ttctatttaa
    12601 tgcgcttatt ttattgtatt ttatgtaata cagttgtttt ttatattata tgtccattat
    12661 tttcctatgg ttattacata tagattttta attatatttg taccattaat ttaaacattc
    12721 ctatactact gcttaagttt tacacctata ccagcttgaa tattttattt ttatttgctt
    12781 aaatagttca actatactgg tttaaatagg ttatctttat ttgcttaaat agttcaacta
    12841 tactggttta aataggttat ctttatttgc ttaaattagt tcaactatac tggtttaaat
    12901 aggtttcctt tagtttaaat aattcaccta tacctacttg aatagttcac ttttatttgc
    12961 ttaaattcta taccaatact ggcttaaaca ttatactacc atgattgtat tgtctatctt
    13021 caactacaat aaaaacaggt tctaaatctt tagtagatgt tatttttaat tctaaaaaaa
    13081 cattatcagt tggtaatatt tttacatcat aattatttaa attattatta gtgatactat
    13141 taaatacatc accttgacgt tcaaaaaaaa cattatcaac tattacaaaa taaacatctt
    13201 cttttctact attttcaacc tctatagtat tttgtttagt tacatttagt ctcatcttcc
    13261 acctcctatt aatattactt aattatatta atacttgtaa ttgttgcatt atgttcatca
    13321 caaggaagag gtttaaaaac aactatctct gctaaataat tctgtggagg atttgtaact
    13381 gaagtagcat tgcaaataaa tttaaaattt gttcctgcta caacttgagt agaaacaata
    13441 agtggttcat aaccaacccc ttctaacatt cctacagctt cattaaatac ttttaaatct
    13501 tcttcttcga cagctctgaa ttttgaccat ccaccaacaa aaagactcat ataaaaacct
    13561 cctttatata tatatttatt tatatatagt agtaccaatt ttctcaatat tcaatatttt
    13621 ctgtgtaact ggtcaatgaa aatgcataac tggataaaaa tacttataaa ataacttaac
    13681 tttacatata aatagcttac tttacatgta ataagattat tctacaatat gaaatacatc
    13741 ttgttttgaa tatattctaa atacaataat ggatattttc aattattatc cttagaaaat
    13801 atccattact taacttgttt tatttaaaat cacatagaga ctttattatt ttttttattg
    13861 catgaatagc tatttcaggt atcataaggt gatatacccc ctccttgaaa ccacaaaaaa
    13921 catgacaaac tttacgaatc ttggcttaaa aaactacagg tacattcctt atttgtggat
    13981 tggagtagcc ataacaagca aattttacaa tttttatgat gacaaaatca ataaaatcgc
    14041 ttagtatata acacgaaaca ctacttgttg ctattaagaa tagttagata ggaagtatgc
    14101 attataccaa taaaggaatc aataaagaaa gttaaaacat ccctacaaat caaaaatctg
    14161 ttacattttc tatatttaaa cgttatttac ttacaattgg agaaataaca ggttttcctt
    14221 ctcggtcaag taaaggcgta aatccggctg catttccata atagtggaat acatagttca
    14281 cacctgtttc tttatccacc caaatctccg ttgcatttat ttttccttgt gaatatactt
    14341 tttcaaatct atcttttccc attttataat cccccatatt tttgattggt ctataccttc
    14401 ttcacttaag tcgaaaaaat acataactta tttttttaat tctaatatga acacgtaaaa
    14461 atgtcaaaca gcttatttaa tttcataact tcatgttact gttcgtaacc ttgtttgttt
    14521 acttacacta cattacagca ccattttacc ctttacatga ttgtattgca cacagcaaaa
    14581 atgcttactt tattggattt ttcaactaaa aaaaggtttt tttctccaca gaacatttac
    14641 tattttttat aatatatcta attttactct catatcaaca tttaatgaag atataacctt
    14701 tttcaaatat tgtaaagact ccttaaaaat attagaaaaa gattctgcaa atctctcaac
    14761 atctcctttt tttaaatacc taagtatctc aaaacataca tcaaaacgtg ttttcaaaat
    14821 ttcattatat tcttcatgaa aaaatctctc taaatagatt ccaaatatca tatattgttt
    14881 taaatgcaaa aatgtttcct gtaatacata aatttgtgta taatctacta tcttacaaag
    14941 taaaacaaaa tctggataaa ccttacactc ttcacaatta ttttgtaagt accttaaatg
    15001 agtttctatt ttatctacag ctatcaaatc cattttattt cctagtaaaa gtactacatc
    15061 ctcaagagta atcgtaacaa actccatagc atccaaaagc aaactcaacc ttttagctcc
    15121 ttcttctgta tttatataat catcatcata ttcagaaaaa agtgttacat atgttccctt
    15181 gccattaatt gttttagcaa ttcccaattc atttagctca ttataggcac ttctaactgt
    15241 tggaagtgat acattatatc tattaattat cgaagtaatt gatggtagac aatctcctat
    15301 tttaatattg ccttcaaaaa tttgacaaat tacatctgaa acaattaagt catataaata
    15361 acatttttcc aacgttacag aattaaaact acttgtatct atactttgtg gagcacgatt
    15421 ttctactatt ttaagtatgg attctctaca agctttatac atatatgtta atagtttttt
    15481 aaattcaatt agattcccgt ccaatccata taagtaagtt tttcttacct gctctatgta
    15541 tttattacga aaaaagtcta tacaacaatt atccaagtta tcgaaattat aggccaattg
    15601 tgaaataatg acatcatgct gaataatctc acaaaaatgg tgaatcagga aattatttgt
    15661 tttacttatt agtttattta tatattttag cctttgttct ttaaagacta aataattaga
    15721 actatttttt tccatacaat cgataatctc gtttaattct tcaaagtctt tagatgtaaa
    15781 tcttttaata ttgtacatag caatagaagg aaatattaaa ctaaaaatct tatatatttc
    15841 tgatatttgt aaaatcttct catataataa gtcagattct attgattgtt tactcgtact
    15901 ctggattaaa actggtctag aaccagcttt tgtctctata aagccctttt ctgagagcat
    15961 ttttattaca gtttttatag tattttttga taccatatat tcttctgaca tgctttttat
    16021 agaatcaagc ctatctccaa tagtaatttt accagtataa atttgttgaa ttatatgctc
    16081 aaaaataatt aaatattgtg gttttctctt catatttttc ctcttcgata aattagattt
    16141 attgtttttt gtaaggtata tttactttta tttaataggg caaaatagtt gcttattttt
    16201 tcctcataat ctataattaa tataaaccat ttatatatat ggtctaagtt ttataatact
    16261 gtaatgcagt aaaatagcat attgtagcta tactacagca aattaacata ccattgtagt
    16321 atattaattg gaataatttt aagtacttca aaattccagc aaaacatata tgtgtttttg
    16381 atattatgta tattcgttaa tagaatagct atacttgttt ta
//
LOCUS       DAEOSP010000074        15989 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.074, whole genome shotgun sequence.
ACCESSION   DAEOSP010000074 DAEOSP010000000
VERSION     DAEOSP010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15989
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.074"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(133..1323)
                     /locus_tag="KRH54_001915"
     CDS             complement(133..1323)
                     /locus_tag="KRH54_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG8579573.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENSENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKISYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(1583..2014)
                     /gene="dut"
                     /locus_tag="KRH54_001916"
     CDS             complement(1583..2014)
                     /gene="dut"
                     /locus_tag="KRH54_001916"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBG8579574.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            2127..4355
                     /locus_tag="KRH54_001917"
     CDS             2127..4355
                     /locus_tag="KRH54_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBG8579575.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(4466..4951)
                     /locus_tag="KRH54_001918"
     CDS             complement(4466..4951)
                     /locus_tag="KRH54_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8579576.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            5203..6426
                     /locus_tag="KRH54_001919"
     CDS             5203..6426
                     /locus_tag="KRH54_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG8579577.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            6430..9564
                     /locus_tag="KRH54_001920"
     CDS             6430..9564
                     /locus_tag="KRH54_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBG8579578.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            9598..10896
                     /locus_tag="KRH54_001921"
     CDS             9598..10896
                     /locus_tag="KRH54_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBG8579579.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYDRYKKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDSLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(11185..11430)
                     /locus_tag="KRH54_001922"
     CDS             complement(11185..11430)
                     /locus_tag="KRH54_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBG8579580.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(11644..14271)
                     /locus_tag="KRH54_001923"
     CDS             complement(11644..14271)
                     /locus_tag="KRH54_001923"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG8579581.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(14332..15174)
                     /locus_tag="KRH54_001924"
     CDS             complement(14332..15174)
                     /locus_tag="KRH54_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBG8579582.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(15181..15819)
                     /locus_tag="KRH54_001925"
     CDS             complement(15181..15819)
                     /locus_tag="KRH54_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8579583.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
BASE COUNT         5605 a         2538 c         2297 g         5549 t
ORIGIN      
        1 aaataaataa aaagcaggta tcaatcaaat agtaacatgt attatactat acattactat
       61 caatattgta cctgctttca tattataagc aataaagaaa aatctattta ttaggtttta
      121 taataaaaac aattataaaa ctcttcttgc atttacatat ctacttgaat agtagctact
      181 atttatatta gatattgtta ctttttttga tgaacctgaa gatgcatgta tcatatctcc
      241 atttcctatg tatattccaa catgacttac agaaccatta tttgaacctt gtgtatcaaa
      301 gaatactaaa tctccacttc taaggtctcc tctatttaca tatgttccat acttactttg
      361 gtctctagat actcttggta tactaactcc tacagatttt ttcataacgt actgcgtgaa
      421 tccagaacag tcaaaactat ttggaccttc tgctccccat acatatggtt ttccaagtag
      481 tgtctttgca aaacttatta cactatcagc tccttgactt ggagaattag tcgaataaga
      541 tggaattgaa gtatcaacag aacctttttc agcaaggtat tgacttgaca catatccagt
      601 ttgattgcca taacttattt ttgtccatcc tccactttca gaaatacttc caacctcagt
      661 accataactt aatgtagcta ttttagaatg acttgtacct ggtcctgttc taacgttcaa
      721 tgactttgct gttacaacct ttgtaccctt tacaaggtca tttgaactgt tttcagaatt
      781 ttcatttgta gagtcattta cactagttcc taaatattta cttgaaacat aacctactct
      841 accattagaa cttatttttg accatccatt tgactcagat atgtatccga cttcagtccc
      901 atatcctagt gttgatattt tagaactact tgtacttggt cctgttctaa aatttaatcc
      961 ttttgctgtt acaacttttg tcgacttaac tgaagtatca ctactttcat tgctacttcc
     1021 aaggctattt attgctacat aatttccatg cacataacct gtgtttccat tatatttaac
     1081 ttttacccaa gaaccttctt ttcctacgta ctcaacttta tcacctttgt aaagtttccc
     1141 cattgattca tggtttgtac ctggtccttt tctaaaattt accgcattcc ctgttattgt
     1201 tctgctttct atactcacac tctctacttc agactcatta gcatatgcta aattccccat
     1261 agtaggcatt attatcgcag ttgccattat actggctgct attctctttt gattaaaatt
     1321 cataactttc cactcatctc ctcttttcta ctatctttat ttcacttcaa aatataaaag
     1381 aaataattga cactatcaaa atatcaatta tttcttttat attaatatta ttacaaattt
     1441 gtaactattt gtctatagta atatttgctt accataaatt accatgtata aattttaatc
     1501 tttaatagcc tgtaatttag taatatagcc atcttttacc ttttttaaaa atggctatat
     1561 tttttaactt ttttgtaact ttttaaaatc cagttgagcc aaacccacct tttcctcttt
     1621 cggagtcact taattctttt acttcttcta tgttaatatc atagataggt tttactatca
     1681 tttgagcaat tttcatatgc ttttcaacct taaaatcatt tttaccatga tttattaata
     1741 taattttaag ctctcctctg tatccttcgt caatagttcc tggtgtattt aatacagtaa
     1801 cagaatgctt taaggcaagc ccacttctag gtcttacctg cgcttctgtc atagtaggaa
     1861 gctctataca tatcccagtt ttgataagtt tagtctctcc aggaggtatt acaacctctt
     1921 ctatagaata caaatccata ccagcatcac ctttgtgagc aaaattaggt attatagcat
     1981 catcatttaa tttttttact tttaaattgt acatcttata tctcctttca actttctata
     2041 aatattataa cttaaatttc gacctcttga agttttttat ttttttgtgg taatataatt
     2101 attagtgttt taaaggagga ttttttatga aacttgtact tgctgagaaa ccatctgtgg
     2161 caaaaactat agcatcattt ttaggtgcaa agaccagaca agatggttac tttgaaggaa
     2221 atgattatat aattacttat gcagttggac acttagtatc attatatgat atgaaggatt
     2281 atgataaaga taaatactct ggctcttgga aaatgaacaa tttcccattt gttcctgagg
     2341 ataagtttaa atttaagatt gatagttcta aaactaaaca attcaacact gtaaaaaagc
     2401 ttttgaatag aaaagatgta gaatatgtaa taaatgccac tgataatgac cgagaaggag
     2461 aacttatagc atttttaata tttttactgg caaaaaataa aaaaccagta aaaagaatct
     2521 tggtaaatga atggacacca gaagatatta caagaggtat aaaaaaccta aaagatgaag
     2581 atgaaatgag aaatcttcaa gctgctggat atactaggct tattactgac tggttgattg
     2641 gaataaattt cacttctgtt gctacattga aatacggaaa tgggaaacta ttaaacatag
     2701 gtagggtaat tcttccaaca gttaaattag tctatgatag agatatggaa atactaaact
     2761 ttgttcctaa aacttattat gaaatagaag ggcattttaa agctgaagct ggtgaatata
     2821 aaggtaaata tgttaagggt aaagaaagta aatttgatac acttgaagat gcaaataaaa
     2881 ttatagcttc aattacatct gaaactggta aaatactaga taaaaaagtt actatgtcaa
     2941 aggaatatgc accaaaactt tttagtttga catcacttca aggatatata acttctaagt
     3001 attccaattt tacatctgat aaggttttaa atgtctgtca atctctctat gagggtaatg
     3061 gtaagggtgg atacataact taccctagaa ctgattccat tttcttagaa gaaagtttgg
     3121 cttcaaaagc ttctcaaact ttagataaat taaaggttgg tcttgaatat gaaaataaaa
     3181 ttaaattttc taaaactaaa agagtttttg attcttctaa agtagactcc catagtgcta
     3241 taattccaac ttatataatt ccaaaaagtt tatctaagga tgaacaatta gtttatgatg
     3301 ccataaaaga tagatttgta gcaaacttta tgcctcctgc tgaatatgag aatacagaaa
     3361 ttaagactga agtagataat tgtactttct taacaaaagg taaggtgctt aaatcaaaag
     3421 gataccttga ggtatataat aaggaagaaa aaaacgattt acttccactt gtaaataaag
     3481 atgatgttgt agatgtgtta gaaataaaac cattaactaa gcaaactact ccacctaaac
     3541 cgtatactga ggacacactt ttaaaggcta tgaaaaattg tggtaaaaat gtgcctgaag
     3601 aagatacaac agtgctatct ggatattcga ttggaacatc agccacacgt gctgatgtgt
     3661 taaagaagat aagtcaagtg ggctatgtaa agaaaaaagg taaatcttat tctataacag
     3721 accttgggaa aaatttagtt gaaatttttc ctgttaaaga cttatttgat gtagattata
     3781 ctggaaaact tgaaaaaagt ttaagcgata ttcaaaaagg aaagtataca agaaaagaat
     3841 atcttactaa tatcattagt tttatatatc aaaatgtcaa tattataaaa caagatgctt
     3901 caaaaaatat aaacacagaa gcatatactt tcgattcaaa aactaaaaaa tttactaaaa
     3961 caaaagaaaa aaaacagaaa aaagatgtaa ctaatactga aaaagaagtt gctatatcaa
     4021 aggattctaa taacaaaaaa gaagttaaaa atggtgatga aaatcaatct cttggcaaat
     4081 gccccatttg tcaaggtgat gtactagaat ttgacaaagg ctttgcttgt aaaaatcata
     4141 aagaatgtaa atttgttatt tggaaaaatg ataagtattt aagtctttat aaaaagaaag
     4201 ttaacaaaac aatggtcaaa aatattttaa aaaaaggtga aactaaagta aaatctttaa
     4261 ctgctaaaaa cggtaataaa tttgatgcta cattaaaata tgataaaaat ccagatactg
     4321 gctatttcaa ttggaaaata gagtttgaca actaaatatt cataagaaaa gaaatagcag
     4381 tagtataata atctatatta tattactgct atttcttttc ttatgaagtt tcaacttttt
     4441 taatagtgac ttttacaatc acactctaac ctaatttctt tactatttct gataacttta
     4501 atgtatgttc atctaataaa gttagttcct cttcagaaag attagataat ttttttacta
     4561 gagattcttt gagaatagct tttactgcat caaataattt aaatgcttta tctgttaatt
     4621 caaccctaag aatccttcta tcttttgggt caggaaattt attgactaaa ccatgttcca
     4681 ctaatctgtc aataatcgga gtcatatttg attttaaaat tcctaaatta gaagctattt
     4741 gagaaattga tgatgaattt gatgtcataa gataaagaat taccttagca tgtgatggag
     4801 ccattggaca ttcttcaaaa taagctttca ttatatcaga gttattttga aactttttta
     4861 acaaatcgct ttctttaaat atattatttt caagcacaaa taaaaaatct aaaaaagagt
     4921 ccgctatttt aagtatattg ttgttatcca tatttgtaac atcccttcaa tagataatat
     4981 aataaaacat tgttgtttaa tatagtataa attataaact attaataaat ttaaaacaag
     5041 aaaaagttat tgactcataa taattataaa tttataatca aattagagaa ttataaattg
     5101 ccaataaagg gaagtttttt aattagtcta aaactaaact taaattataa gacactttaa
     5161 attctacaac aagctagtgt gcaatatata ataggaggag atatgaaaag atatttaata
     5221 gttttcttaa tgtgtagttc actgttttta gttggatgtg gaaaaacaga agaatctgaa
     5281 cctgagaaac caatagcagt ttctgttcaa aaagcaatag gtggagaaat tgaaaacaca
     5341 aattctttct caggaactac aaaggttaaa gatgaaacag ctgttactgc acagactgtt
     5401 ggcacagttc aagaagtgta tgtaaaactt gggcaaaatg taagaaaagg agatgaatta
     5461 ttgagtataa gcagtccaga gctagaaaat agtgtaaagc aaagtaaagc ctcactagat
     5521 ttagcaaaag caagttattc aagtgccact ggtggttcat tagaggcaca agtaaatcag
     5581 gcaaaaacag ctcttgacaa tgcaaagatt caatatgagg agtctcaaag aaattatgac
     5641 aataataaga ttctttatga acaggaagtc attagtttag accaatttaa aaaaatagaa
     5701 ttttctttag agcaaacaaa acaacagtta gattctgctc aaagagcata cgatacagca
     5761 acttcaaaaa gtatacctca agctaaagca cttgctaaaa aacagcttga ccaagctcaa
     5821 gtatcatata atttagcaat gagtaattta gacaaattaa cattaacatc tccagtagat
     5881 ggtacaataa cagctaaaaa ctttgattca aaggaaatga taacacaatc acaaccagct
     5941 tttattattt ctaatcctaa tatactagaa gttgatttaa atgtagcaga atcagatata
     6001 ggtaaattta agaaagatgg aaatgtagat gttataattg aagaccaaag gattttagga
     6061 aaaatagatt atgtaccttc agtagttgac cctcagacat ctttatatcc tgttaaagta
     6121 cttgtaaata atacaaacaa caaatttaag gcaggtatgt ctgctcaagt aaacttaagt
     6181 gttgaaaaag aaaatggtgc tgttacagta cctaaaaaag caatatttga agaaaatggt
     6241 aaaaaatatg tatacatagc aacaaaagac aatatagcaa agaaacactt ggttcaaact
     6301 ggaattgtaa cagaggataa aatagaaatt aaaagtggtg taagtgataa agatacagtg
     6361 attatcggag ggataagtct tatttctgac ggaactaaga tatttcctgt agaaaaggag
     6421 aagtagacta tgaatttaac acagacttcc gttaaacgtc ctctcacaat cataatggtc
     6481 tttttagtat taatagtttt tggtggtatt ggatataaaa aaatgtcaat aaatctaatg
     6541 cctgatatag aaataccagt tgtaatggtc atgactactt ggacaggtgc tggtcctcaa
     6601 gacgttgatg aacaagttag tcaaaaagtt gatgaaagtt tatctgccgt ttcaaatgta
     6661 aaatctacta tatcaagttc acaagaaagt gtttctatgg tagttgctca gtttgaattt
     6721 ggaactaatt tggatgaaat aatgaatgat gttaggtcta aagttgatgc attacagacc
     6781 agcctcccag atgatgcagc taaaccaaca gttttaaagc tggatatgaa tgcacaggct
     6841 ataggtcaac ttgtcatatc tggaggaaat gaaaatagtt cacaagcctt aagaaaatac
     6901 gctgaagatg taattcagcc taaaatagaa tccatagatg gtgttacttc agcagacctt
     6961 aaaggtggag aaaaagcaca ggttaatgta atagctgacc cagctgttct ttcaaattat
     7021 ggagttagtt tatccactat aaaaggtgtc ttatcctcta gtaataaaac attcccttat
     7081 ggttcaatta ctcaaggtga ggataaaata gttttaagag cgattgataa attagaatcc
     7141 ctagatgata taaaacagat tcaaattcct gtaaaaggag gaaatacagt tagattagat
     7201 aatctttgta gtgtagatta tggatatgca gacaaagata gtatttatag atacaatgga
     7261 aaagacagtt tggtaataga tatttctaaa caacaagatg ccaacactgt aaaagttatg
     7321 cagagtgcat acaaatacgt agatgaattg aataaggaaa atcctcaatt taaaattaaa
     7381 attgctaatg atacaagttc atacataagt gaatcaataa acagtgtaat gagcaacttg
     7441 cttataagtg ctgttatcgc atttattgta atatttgctt tcctaaaaag tgtaagagct
     7501 tcactagttg ttgcagttgc aataccaatt tctataatag gtgctatagc aattctctac
     7561 tttactggtg aaagtttaaa tcttattact gcaagttcat tggtaatttc agttggtatg
     7621 gttgttgata atgctacagt tgtaattgaa aatatattta aatataggaa aagtggcaga
     7681 ttgagtcttg atgattgtgc tattgaggga acaagaactg taacaaatgc aatacttgca
     7741 tcaacattga ccacaatagc aatattctta cctatcatgt ttacagaagg tatagctaaa
     7801 ataacttttg gggcattagg taaaacacta atagcttcac tagcattctc ctttataggt
     7861 gcaattacac tcgttcctag tgtatttgca aaattaagca gaggaaaaaa tagtcaaaag
     7921 atgcaagaaa aagatagtcc tatatttgat aaggtaagtg aagcatataa aaaattatta
     7981 agtgtatgtt tgagacatca acgcttagtt gtaatatcaa gtttacttat ttttgtagct
     8041 tccttatttg gaatgggatt tataggaatg gactttatgc ctgcagcaga caagggtgaa
     8101 ttatctattt ctatagaact tccaaaaggt cttagcctag aatccaatga ttactatgta
     8161 tctatgactg agaaaaaagt ctcagatatt ccagaaatta aaactttaat aacaactttg
     8221 acatcagatt caaataatag taaagcaagt atagcaattg aattagaatc tcaaaaaaat
     8281 agaaaaagaa ctactgatga aatagaaaaa gagataattg atagagtggc tactgtacca
     8341 gattgtaaaa taaatgtaag tcaaaacagt agtgttatgg gtggagctgg aggagcagac
     8401 ttcacattag aaatgaaagg tcctgattta gatgtattaa aaatactggg aaaagaagct
     8461 gaaaaagaat ttagctctat aaaaggattt agaaatgttg aaacttctct tacagataca
     8521 acacaagaag ctcaatttac tatagataaa cataaggctc aagaatatgg tgtaaatact
     8581 gctgaaattg caggcatgtt acgtacagca ataaatggtg actctgtaac cactgctact
     8641 attgatgatt acaaagtgga tgtaaaccta aaatttaaag cagatactat tgatagctta
     8701 gaagatataa agcaactaaa agtaaactct gcaactggaa aagaagttcc tcttggtgct
     8761 ttttcagata taaaaatggc agatggtcta caatctatat ctaggactga tggagatttt
     8821 tctgtaaata taactgctac acttgacaat ttagatgctg gtactgctac aaaactagca
     8881 ctagaaaaaa cgaataaaat ggtaattcct aaagattact ctataggtgt tggtggtgaa
     8941 acagaaatga tgaatgaatc tatggcaagt cttggatttt caatggtcat agctatagca
     9001 ttagtttata tggtaatggt agctcagttt gaatcattta gtaaaccatt tataatcatg
     9061 ttctgtgttc catttgcatt cgttggagtt gtgctttcgc ttctagttag tggtcaaagt
     9121 ttaagtactg tcggtatgct aggtataatc ttacttatcg gtattgttgt taataatggt
     9181 atagttctta ttgactacat agagcaactt agaaaaacaa atcgtaacaa tgatttaatt
     9241 ggtttggttt caattggttg tgcagcaaga ttaagaccag tcttaatgac tacagcaaca
     9301 acaattttag gtatgttgcc aacagcacta tctcttggtg aaggtggaga aactatgcaa
     9361 ccactagcca tagttataat gggtggtcta actgtatcta cacttataac tttggtgctt
     9421 atacctacta tatacttaat atttgataaa tgggaaaata agttttcaga caaaatgcct
     9481 aaaatatttg gtaagataac agataaaata ggaagtataa aacgtaaaat aataaagaaa
     9541 ccaaaattga aagctaaagt ttaaatagaa tggagggtga ttgtattgaa taaaaaaatg
     9601 agcaaaatag ttgcaattgg ctgtggtgtt ggtataataa ctgccaattt aatcccagtt
     9661 tacgcagata ataatacttc ttctataaac aaagttgata attctatttt aagtgactca
     9721 aataaaagaa catctgaaaa ggaaattttg actgctagag aagctgttag tgctgcactt
     9781 aaaaatagtg aaaaattaaa aatgaaatca gaagaaataa aaatgttaaa agaaaagtta
     9841 gaagtacaag atgagtttga tagttttact ggaagtgaca actcgtttcc atacgaccaa
     9901 attggacttc ttaaaaatca aagtgaacag gctaaaggct ttatggagga ccaaatagca
     9961 aatgatatta ctaataaatt taacgattta gtttcaagag aaaatgagct agataagata
    10021 aaaaacaatc ttgaaataaa aactaaagaa attaaagata tgaaattaaa aaaagattta
    10081 gggttagtaa catctttaga aactgaatca gctgaacttg aacttcaaac actacaaaat
    10141 actcaaaaag ctaaacttca agaactaaaa aacaatcaag actattttaa gttattgact
    10201 aatatagatt taaataatta tcaattggat aaagaatata gatttgaatc ttttagagta
    10261 ggtggctcag ttgattcata tatggaaggt aaggttaatg aatatttaaa atacgaccaa
    10321 ttgattttag aacgtacaga agaaagcttt aatgataaag atgagaacaa agcagattta
    10381 ccagatagac ctaaccttac aagaccagtt gctcctacaa aaccagagca aggtaaccta
    10441 actgatgatg aatacaaggt tcttatggat aagtatgaaa aagattatga tagatataaa
    10501 aaagaagttg aagcatacaa tcttgaaaga cagacttatg ctgctggtct tactacttat
    10561 gcaaattatc ttcaacaaaa atttaatact gaaaatggtc tagtaacatt agaagacagt
    10621 aaaaaagctc ttaaaaaagg gttaattgat agctatgctc aattacttgc tttagaagat
    10681 acaattcaga ttactaaaaa acaacttgat ttatcagaaa aacaattaaa aaatactaaa
    10741 ttaagatatg atttaggatt aattactcta actgattata aaaaacaagt ggtaagtaat
    10801 gaagatgcta aaaatagcta tgactctctt attgtgaatt ataactcact aaaaaatggt
    10861 atagaaaaac cttggatact taatactgga aaataattct ggtaaataga aagattactt
    10921 taacatacgt ttgattaaaa aatagttatg cacactcgtg gctttagtca tggaatattc
    10981 agaaggcaaa taatagaaat agattagtaa aaatatattt acttataaaa ctgtatactt
    11041 ttatagaggc tatctcaaaa tagagataag cctctttttt attgtttaaa ttataaaaat
    11101 atatttgatt ccatacaaaa agagtgcctt atgaactaaa aagttcattt tgagacaccc
    11161 tcaaaagctt aactctaaat ttaattattt tttatcaaat gtctcatcta ttttacatct
    11221 caccatgtta acaaattcag aaatattata tcctctaagt ttgcatccac atttaggaca
    11281 ataatccagt ttcctagata ccaaagccct acaactaggt tctggacatc ttatttttag
    11341 actctcatct gctactttaa atccacatcg tgggcatata ctacctggac gtgatgatac
    11401 ttcctttcca catttaggac actttgtcaa ctttatcccc tccagtaaac tcaatttaat
    11461 tgatagttga aatttctctc tacaataata tatgcaatat aattaaatac gtttcactta
    11521 caactatata agttaaattt ttataataaa aatcccctat attcaattta caaatactta
    11581 atttgataca ttgaacaata ggggatttta tttttattta gttacatatt ttgcagtcta
    11641 ttattatcta ggatttttta tagatgcttg agctgctgca agtctagcaa ttggcactct
    11701 aaatggtgaa caagatacat agttaagtcc tacactataa cagaactcta ctgaagatgg
    11761 ttctcctcca tgctctccac atattcctag ttttaattct ggtttaactt ctctacctaa
    11821 tttagctccc atcttaacta acttaccaac accattttgg tctagtactt ggaatgggtc
    11881 tttttctaat atttcttcat ctacatattg acctaagaat ttaccagcat catctcttga
    11941 atatccaaat gccatttgag ttaagtcatt agtaccaaaa ctgaagaagt ctgcttcttg
    12001 tgctatttca tcagcagtta aacaagctct aggtatttct atcatagtac ctacagtata
    12061 aggaacttct ataccttctt cttttattat agcgtcaaca gtttctacta tcatttttct
    12121 tattattttt aactcattta cttctccaac taatggaacc attatctctg gagaaacttt
    12181 aacacctgct ttgattgctt ctatagcacc ttgtattata gctttagatt gcattacaca
    12241 tatttcagga taagttattg caagtctaca acctctgtgt ccaagcattg ggttaaactc
    12301 atctaaatca actattctct tcttaatatc acttactttt attcccatag attttgaaag
    12361 ttcttctata gtctcatcat catgaggcaa aaattcatgt agtggtgggt ctagtaatct
    12421 tatattaact ggttttccat ccataacttt aaataactct acaaaatcct ctctttgcat
    12481 tggtaatatt ttttcaagag ctttttctct ttgttctact gtattagata atatcatttc
    12541 tctaactgct ggcaatctag cttcatcaaa gaacatatgc tcagttctac atagacctat
    12601 accttctgca ccaaactcta tagctgcacg agcatctcta ggattatctg cattagttct
    12661 aaccatcata catttgtgtt tgtctaccca gttcattaat ttttcaaaat ttccagttaa
    12721 agatacttct gttttcttaa catctcctag gtacacattt cctgtagaac catctataga
    12781 aatgtattct ccttctttta taactacatc atcaagtgct cttatttctt tattaaactc
    12841 atcaactttt atttctccac atccagcaac acaacattta cccatacctc tagcaacaac
    12901 tgctgcatgt gatgtcattc cacctctagc tgtaaggatt ccttctgctt taaccattcc
    12961 ttctatatct tctggagatg tctctagtct aactaaaact actttttctc ctttttcatt
    13021 tgcttttact acatcatcag cattgaaata aactttacca cttgctgcac ctggtgatgc
    13081 tggtaaacct tttgctataa ccttagcttc ttttaatgcc ttatcttcaa atttgggatg
    13141 taataattgg tctaattgat taggctcaat tctcatgata gcttcttttt catcaattat
    13201 tccagcttca actaaatcaa ccgctacatt tattgctgct gctgctgttc tcttaccatt
    13261 tctagtttgt agtatatata atctttcatt ttctatagta aactctatat cttgcatatc
    13321 tttataatga ccttcaagta tatgagtaat ttttacaaat tcatcgaata cagctggcat
    13381 tacatctgca agagttgata tattttgagg agttcttata cctgcaacaa cgtcttcacc
    13441 ttgtgcattc attaaaaatt caccaaataa tttattttct cctgtagctg gatttctagt
    13501 aaatgcaaca cctgtaccac tagtttctcc catattacca aataccattg attgaatatt
    13561 tactgctgtt cctaagttat gagctatatc attcaattta cggtatacga ttgcacgagg
    13621 attattccat gatctaaata cagcctctat tgcaagcatt aattgctctt ttgggtcttg
    13681 tgggaaatca ttttttattt cttttttata aattgcttta aattctttta ctatttcttt
    13741 taaatcatca gttgtaagtt ctgtatcaac tgtgtatccc tttgcttctt ttactctgtc
    13801 taatacgttt tcaaatttat attttggaac ttccatagca acatcagaaa acatttgtat
    13861 gaatcttctg taactatcat atgcaaatct ttcattttgt gtagcctctg ctaagccaac
    13921 aacactattg tcattaagac ctaggttaag aattgtatcc atcattcctg gcatagagaa
    13981 tactgcacca gaacgaactg atactaataa tggattctta ttgcatccta atgttttatt
    14041 taaatctttt tctaaagtag ctaaatgctc ttctatttct tttattatct ctgctcttat
    14101 actttcattg ttaacataat aatcattaca cgcttctgtt gttatagtga atccaggagg
    14161 gacaggtaag cctatcttag tcatttctgc taaattggca cctttacctc ctagtaaaga
    14221 tttcatgtct ttgcttcctt caccaaaact ataaacatac ttagtttcca ttgttaaatc
    14281 ccccattaat ctttttattt taatttatat catttttatt aatagacatt attaaatttc
    14341 tatttctttg tatatcttta gaccattttc tttcattatg tctaaaatta tacctgctgt
    14401 ttcttctatt gccttatttg aaacgtttat tactgggcat ccaattcttt tcattatctc
    14461 ttctgaataa tctaactcct gaagtattct ttctaaatta gcgtagtttg ctttactgga
    14521 caatcctaaa gcttttaatc tttgggttct tatctcattc agcttttcag gtgaattagt
    14581 aagacctatt atctttttag tatctatctc aaacacttct ttaggaattg gtatttctgg
    14641 aactaatgga acattagcta cttttatatt tttattagct aaatacatac tcaaaggagt
    14701 tttagatgtt cttgatattc ccactaaaat tatgtctgct tgaagaacac ctctagggtc
    14761 tttcccatca tcatatttca ctgcaaattc tatagcttca actctcttaa agtaagattc
    14821 atctaatttt ctaattatac cagcttctct tttaggtttt cttcctgttc tcttgcttat
    14881 ttctcttagt atatctgtca ttaaatctat acaactaaga ccttctcttt cgcaatatct
    14941 ttcaacaata ctgcataatt cttcatcaac taatgtatat acaattaaag cgttttcagc
    15001 ttttgcattc tccaaagttt caagtagtac tcctttatta atcatgtatg gaaatctttt
    15061 tatttcataa gtttcattca cataaaactg agacatacat gcctttgcaa cttgttgtgc
    15121 agtctcccct actgaatctg atactgcata tataattagg tttttcacca gcatacctcc
    15181 ttaattgtca actatttcta agaatagctt agttatgttg gtttttgata ttctcccaac
    15241 aactctcata tgatttttgt cttctccctt atattcaaca atcggaatcg aatctacttc
    15301 atgttctatt atttttcttg aagctaataa aacacttgct cctttagtta aagtgactac
    15361 atttggagtt cttgtcatta tcatacctat tggcatttta tttatatcag aacctcctat
    15421 agttgctttt agcaagtctt ttctggaaac aactccacat aactcttcat tctcatctat
    15481 aataactata ctccctacat cagataaaaa catagtgact attacatcat atatactttc
    15541 atccttctta gctagtacag gcatgctcat tatatcctct actgtttttt cttttacact
    15601 tttaccaatt aaatttattt cactaactcc tgaataaaaa tatccaactt ttggccttgc
    15661 gtctaatatt cctgtcattg ttaatatagc taaatcagat ctaagtgtag cacgagtcac
    15721 attcaagctg gaggcaatac tttcactcgt tattggttca ttttccttaa ctatatctat
    15781 aatttttagt tgtctttcat taagctgaat aataatcacc ttctttcatt gtgctatact
    15841 ttttatcttt tcgcatctta tatagtatat tatataacta tatataccat tctgtaaaca
    15901 gattttttat tgaatacgat atatttttta ctaagttctc tcgcttaaag atatttatta
    15961 tatattaatg ggggatattt ctttaatta
//
LOCUS       DAEOSP010000075        15969 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.075, whole genome shotgun sequence.
ACCESSION   DAEOSP010000075 DAEOSP010000000
VERSION     DAEOSP010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15969
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.075"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            248..397
                     /gene="rpmG"
                     /locus_tag="KRH54_001926"
     CDS             248..397
                     /gene="rpmG"
                     /locus_tag="KRH54_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBG8579584.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            423..644
                     /gene="secE"
                     /locus_tag="KRH54_001927"
     CDS             423..644
                     /gene="secE"
                     /locus_tag="KRH54_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBG8579585.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            664..1206
                     /gene="nusG"
                     /locus_tag="KRH54_001928"
     CDS             664..1206
                     /gene="nusG"
                     /locus_tag="KRH54_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBG8579586.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1245..1670
                     /gene="rplK"
                     /locus_tag="KRH54_001929"
     CDS             1245..1670
                     /gene="rplK"
                     /locus_tag="KRH54_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBG8579587.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1741..2439
                     /gene="rplA"
                     /locus_tag="KRH54_001930"
     CDS             1741..2439
                     /gene="rplA"
                     /locus_tag="KRH54_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBG8579588.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2491..2619
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2662..3168
                     /locus_tag="KRH54_001931"
     CDS             2662..3168
                     /locus_tag="KRH54_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBG8579589.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3226..3591
                     /gene="rplL"
                     /locus_tag="KRH54_001932"
     CDS             3226..3591
                     /gene="rplL"
                     /locus_tag="KRH54_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBG8579590.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            3983..4771
                     /locus_tag="KRH54_001933"
     CDS             3983..4771
                     /locus_tag="KRH54_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG8579591.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5242..8958
                     /gene="rpoB"
                     /locus_tag="KRH54_001934"
     CDS             5242..8958
                     /gene="rpoB"
                     /locus_tag="KRH54_001934"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBG8579592.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            9000..12485
                     /gene="rpoC"
                     /locus_tag="KRH54_001935"
     CDS             9000..12485
                     /gene="rpoC"
                     /locus_tag="KRH54_001935"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBG8579593.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            12774..13196
                     /gene="rpsL"
                     /locus_tag="KRH54_001936"
     CDS             12774..13196
                     /gene="rpsL"
                     /locus_tag="KRH54_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBG8579594.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13320..13790
                     /gene="rpsG"
                     /locus_tag="KRH54_001937"
     CDS             13320..13790
                     /gene="rpsG"
                     /locus_tag="KRH54_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBG8579595.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            13839..15905
                     /gene="fusA"
                     /locus_tag="KRH54_001938"
     CDS             13839..15905
                     /gene="fusA"
                     /locus_tag="KRH54_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBG8579596.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6250 a         1985 c         3250 g         4484 t
ORIGIN      
        1 ctttactaaa ctgatggatt aaaaggaata acaaaaagag gggttatgct cctctttttt
       61 taacatagaa atacaaaaac attgacacag cataggattt tgtgataact tatacaaggt
      121 aatgattata ggaagttaat aatatcttga atactgtaga tttttaataa atacatattt
      181 taaagatatt atattcacat atattaacaa tatatattat tgtattacaa agttggaggt
      241 gtatcagatg agagttaaag taactttagc atgtacagag tgtaagcaaa gaaactacaa
      301 cactactaag aataagaaaa acaatccaga cagaatagaa ttacaaaaat attgtagatt
      361 ctgtaaaaag catacaactc ataaagaaac aaaatagttt tttataagga tgtgagtatg
      421 gaatggctgc ccaaaaaaat gacggtgcaa aaactaaaaa aaggtttagt ttgtttggat
      481 acttaaaaga aactaaacaa gaattaaaaa gagttacatg gccgacaaaa aaggaactgt
      541 ttaaaaacac aagtatagtt ttaactgtag ttatttcatg cactatatta gtatggggta
      601 tagacactat attatctggt gcactagcgt tattactaaa atagacgaaa agaggtaagt
      661 taaatgtcag aattacaaga agcaagttgg tatgtagttc atacctattc gggacatgaa
      721 aataaagtta aagctacaat tgagaaagca gttaaaacaa gaggtatgga agactgcata
      781 agacaagtag ttgttccaac agaagaagta gtggaaacta ctaaaactgg aaaagaaaaa
      841 actagacaac gtaaagttta tccaagttat gttttggtta aaatgataat aactgatgaa
      901 tcttggtatg tagtaagaaa tactaaggga gttacaggtt tcgttggacc tggttcaaaa
      961 ccagtgccat taagtgaaga cgaagtcaaa gctatgggga tagacacaac agaccctaaa
     1021 gtagtaaata gcgatattga ttttgaaata ggagatacag taaaagtatc tcaaggacca
     1081 tttagtggac aaataggtaa catagaagaa attgacttag aaaatagaga agtcaaagta
     1141 tgcataaatg catttggtaa aaggactcta tttgtaatag agcttgaagg tatagaaaaa
     1201 atctaatact atattataga aaaatctgag gaggtgcgct caaaatggct aaaaaagtta
     1261 taggtcaaat aaaattacaa atacctgcag gaaaggctac tccagcgcca ccagttggac
     1321 cagcattagg acaacatggt gttaatataa tgggattcac aaaagaattt aatgctaaaa
     1381 ctgcagatca agctggaatg ataataccag ttgttataac tgtatatcaa gatagatcat
     1441 tcagttttat aacaaaaact ccaccagctg cagttttaat taaaaaagca ttaaacttaa
     1501 aatcaggttc tggagaacca aacaagaaaa aagttgctaa aatgacttca gctcaagtaa
     1561 gagaaatagc tgaattaaag atgcctgact taaatgctgc atcagtagaa gctgctatga
     1621 gtatgatagc tggtactgct agaagcatgg gtgttgtaat cgaagactaa ttaaaagata
     1681 cacttttttt tataaagtgg gaggctagaa gccgaaatta accacgagga ggaaaataaa
     1741 atggctaaaa aaggtaaaag atatgcaggt gcattacaaa aagtagatag aactaaattt
     1801 tatgatgcat cagaagcatt aacattagtt tcagatatag ctggagctaa atttgatgag
     1861 acagtagaag cacacattaa attaggtgtt gactcaagac atgctgacca acaagtaaga
     1921 ggtgcagttg tattacctca tggaactggt aaaacaaaaa gagttttagt ttttgctaaa
     1981 ggtgaaaaag ctaaagaagc tgaacaagct ggtgctgact ttgtaggagc tgaagaatta
     2041 gttcaaaaaa tacaaggtga aaactggttt gactttgata tagtagttgc tactccagat
     2101 atgatgggtg tagtaggtag attaggtaga gtattaggac ctaaaggttt aatgccaaac
     2161 cctaaatcag gaacagttac atttgatgta gctaaagcta tagatgaaat aaaagctggt
     2221 aaagttgaat acagattaga caaaactaac ataatacacg ttccagtagg aaaagtatca
     2281 tttggtggag aaaaattaac tgaaaacttt actgcattaa tggatgctat aataaaagct
     2341 aaaccagctg ctgctaaagg acaatattta agaagtataa cagtagcttc ttcaatggga
     2401 cctggagtta aaataaaccc agcaagaaca gctgaataat aaaactgttg acacaggttg
     2461 acaaaaatgt tattatatca cttgtcaatg taaaaaagaa tagatttgcc gtagatagtg
     2521 ggtgcgagag cttaatatcc cgccgaggtt ttagataaat tgagattact aaagcttttg
     2581 atgtctactt ttggcaacaa aagcttttta taatgttgcg gtaaatacta agcctatttg
     2641 gtgaaggagg tgcacatcta tatgagaaaa gctatagaaa ttaaatcaga agttgtttct
     2701 gagatagttg aaaagttaca aaaatcttct gctgctgttg ttgtagatta caaaggatta
     2761 acagttgaag aagtaactga gttaagaaaa caaatgagag aagctggtgt agattacaaa
     2821 gtatacaaaa atactttagt tagaagagca gctaaagaag ttggtataga gcaatttaat
     2881 gatgaattat tagttggaac taatgccata gcatttggat atgatgatcc agttgctcca
     2941 gctagaatat taaaaggatt catggattct catccaaaaa tgaaattaaa gatgggtatt
     3001 gtggaaggtg cattctatga tgaatctaag attgttgaaa tggctaacat accatcaaga
     3061 gaagttctta tcgctaaatt acttggtagc ttaaaagctc cagtatcaaa cttcgcatac
     3121 ttaatagatg caatagcgaa aaaggcagaa ggtcaagaag aagcttaatt gcttaactaa
     3181 aaaattataa atattaattt gaaataaaat tcggaggtgc taaaaatgac aatagaacaa
     3241 atattagaag ctatagaaaa tatgaaagtt ttagaattaa atgaattagt taaagctgct
     3301 gaagaaaaat tcggtgtatc agcttcagct ccagtaatgg tagctggtgc agctgctggt
     3361 ggaccagctg ctgaagaaaa aactgagttt gatgtagtat taactgacgt tggttcttca
     3421 aaagttggag ttataaaagc agttagagaa ataacaggat taggattaaa agaagctaaa
     3481 gaagtagttg acaatgctcc taagacagtt aaagaaggag cttctaaaga agaagctgat
     3541 caaataaaag agaaattaga agctgctgga gctaaagtag aagttaagta gtttctttaa
     3601 aaagataccc attgggtatc tttttttatt tataaaaaac tataaagaaa gtatttggaa
     3661 ttataaagaa agtattttta taaaataaaa aaaatttata taatagtact aaattaaggg
     3721 tatcatatat ggtgtaatgg tattttaatt taacattatt atcaaattaa aatataaaca
     3781 attgttgtaa tgtctatata taaagtatat aattaaacta aaatgtaaaa ttttacattt
     3841 tagtttaaaa aaatacacga gtgttttgta aataaaatgt aataaataat taattaacaa
     3901 atataaattt taaaataagt tagatattta gaaaaatcga aatttgtagg atttaaagga
     3961 catattcaag gaggagttaa taatggaaaa attacaagga aaaattgcag tagttactgc
     4021 agcaacaaaa ggtattggat tagcatcagc agagatatta gcaaaaaatg gagcaactgt
     4081 gtacttagca gctcgttcag aagaattagc taatgaagtt ataaataaaa taagtgcaga
     4141 aggtggttgt gctaagtttg tttactttaa tgctcgtgaa gaagagacct ttacctcaat
     4201 gatagaagag gtagttaaaa aagaaggtaa gatagatata cttgtaaaca attttggttc
     4261 aacaaaccct tctcttgaca aagaccttgt aactggggat acagataatt tctttgacac
     4321 agtaaatact aatttaaaaa gtgtgtattt accatgtaaa gcagcaattc ctcatatgat
     4381 aaagaatgga aaaggaagta tagtaaatat atcaagtata ggttcagtat tacctgattt
     4441 atctagaata gcttactgtg tatcaaaagc agcaatcaac tcattaactc aaaacatagc
     4501 tacacaatat gctaaagaca atgttagatg taatgctgta cttccagggc ttattgcaac
     4561 taaagcagca ttagacaata tgtctccaga gttcataaaa gaatttttaa agcatgttcc
     4621 tttaaatcgt ataggggaac cagatgatat agcaaaagct gttctattct atgctagtga
     4681 tgactcatca tttataacag gggatttact tgaagttgca ggaggatttg gtttacctac
     4741 tccacaattt gcagataata tattaggata attaaattac taatatgcaa tgaaagctta
     4801 tgcattagtt gtagtagaaa tgatattttt ataaagacca tagatataat ttatattgga
     4861 aaggtttgag gttcaaaatt ccaagttaaa tttatttatg gtctttattt tgtatatttt
     4921 aataaattgt atatatttgt ataaatgtaa ttatttacaa tcattttaat atggaattat
     4981 aattaaatag gggggatggt acttgtataa aattttttat agaaaattag acctataaat
     5041 gaaaaatatg gattttatat tggaaaattg tacataatac ataatatatg cttaaaataa
     5101 tttacaaaaa atcaaaaata atgcatagtt tacatataag ataaaaaata tattgaaaaa
     5161 tacttgacat tatgctaaaa ttattagatg cgttagaatg aactattgtt ttatttttaa
     5221 ttattgagag gtgaaaagtg aatgccacat cctgtcacga taggtaaaag aactagaatg
     5281 agcttttcta aaattaagga aatagctgat gtgccaaatc ttatagaaat tcaagtagat
     5341 tcctatgagt ggtttttaaa agaaggttta aaagaagtgt ttgatgatat ttcaccaata
     5401 gaagattata ctggtaatct tatattggaa tttgtcgact attctctaga tgataaacca
     5461 aagtacgata tagaagagtg taaagagaga gatgctacat attgtgcacc tctaaaggtt
     5521 aaggtaagac ttataaataa agaaacaggt gagataaaag aacaagaagt gtttatgggt
     5581 gacttccctt taatgacaga aagaggaact ttcgttataa atggagcaga aagagttata
     5641 gtaagtcaat tagttagatc tcctggtgtt tactatgcgg aagaaagaga taaaacaggt
     5701 aagagattga tttcatctac tgtaattcct aatagaggag catggttaga atatgaaaca
     5761 gattctaatg atgtaatatc tgtaagagtt gatagaacaa gaaaacaacc agttacagtt
     5821 ttacttagag ctttaggaat aggaacagat gcagagataa ttgacctttt aggagaagat
     5881 gagagattat ctgctacatt agaaaaagat aatactaaga cagtagaaga aggtcttgta
     5941 gagatataca aaaaattaag accaggtgaa cctcctactg tagaaagtgc atcatcttta
     6001 ttaaatgctt tattctttga cccaaaaaga tatgacctag caaaagttgg tagatataaa
     6061 ttcaataaga aacttgcttt atgttataga ataatgaata aaatttcagc agaagacata
     6121 ataaatccag aaactggaga agtatttgta aaagctggag aaaaaataag ctatgatttg
     6181 gctaaagcca tacaaaatgc aggtataaat gtagtaaatc tactaatgga tgatgataaa
     6241 aaagttagag ttataggaaa taattttgtg gatattaagt ctcacattga ttttgacata
     6301 gatgatttaa acataaaaga aaaagtacat tatccaactt taaaagaaat tttagatgga
     6361 tatagtgatg aagaagaaat aaaagaggct ataaaatcaa gaattaaaga acttatacca
     6421 aaacatatat tattagatga tataatagct tcaataagtt atgagttcaa tatattctat
     6481 aatataggaa atattgatga tatagatcac ctaggaaaca gaagaataag atctgtaggt
     6541 gaattgttac aaaatcaagt tagaattggt ctttcaagaa tggaaagagt tataaaagaa
     6601 agaatgacgg ttcaagatat ggaagctata acgcctcaag cgttagttaa cataagacca
     6661 gtttcagctg caataaaaga attttttggt agttctcagt tatctcagtt catggatcaa
     6721 acaaatcctc tatctgagtt aacgcataag agaagattat cagcccttgg gcctggaggt
     6781 ctttcaagag aaagagctgg attcgaagtg cgtgacgttc accattcaca ctatggtaga
     6841 atgtgtccaa tagagactcc agagggacca aatatcggtc ttataaactc tctaggaact
     6901 tatgcaaaaa taaatgaatt tggatttata gaatcaccat atagaaaatt tgataaagaa
     6961 acatcaacag ttactgatga aatacattat ttaactgctg acgaagaaga tttattcgta
     7021 agagctcagg caaatgagcc attaacagaa gatggtaaat tcgtaaacca tagagttgtt
     7081 tgtagaactg taaatggtgc tgttgaaatg gttcctgaaa gtagagtaga ttacatggat
     7141 atatctccta agcaggttgt atctgttgcc actgctatga tacctttctt agaaaatgat
     7201 gatgccaacc gtgcccttat gggagcgaac atgcagcgtc aggcagtgcc tctagttaga
     7261 agagaagcac caattatagg aactggtata gaatacagag cagcaaaaga ctctggagca
     7321 gttgttgttg ctagaaattc tggtatagca gaaagagtaa cagcagatga aataattata
     7381 aaaagagaag atggaaacag agatagatat aatctactta agtttaaacg ttcaaactca
     7441 ggtacttgta taaatcaaac acctataata aataagggtg accagataat aaaaggtgat
     7501 gttatagcag atggtccagc aactgattta ggagaagttg cacttggaag aaactgtctt
     7561 atagcattca tgacttggga aggttataac tacgaggatg ctatattaat aaatgaaaga
     7621 ttagttaaag aagatagatt atcaacaatt catatagaag aatatgaatg tgaagctaga
     7681 gatacaaaac tagggccaga ggaaataact agagatatac ctaatgttgg agatagtgca
     7741 attaagaatt tagatgacag aggtataata agaataggtg cagaagtaga ctcaggtgat
     7801 atattagttg gtaaagtaac tccaaaagga gaaactgaac ttactgctga ggaaagatta
     7861 cttcgtgcaa tatttggaga aaaggctaga gaagttagag atacttcact taaagtgcct
     7921 catggtgaat ctggtataat agttgatgta aaagtattta ctagagaaaa tggagatgat
     7981 ttatctccag gagtaaatga attagtaaga tgctatatag ctaaaaagag aaaaataaaa
     8041 gttggagata aaatggctgg tcgtcatggt aataaagggg ttatctcaag agtattacct
     8101 gaagaagata tgccattcat ggaaaatgga acaccactag acataatact taacccacaa
     8161 ggtataccat cacgtatgaa cattggtcag gttttagaag ttcatttagg acttgctgct
     8221 aagacattag gatggtatgt agcaacatca gtattcgatg gtgctaatga gtatgacata
     8281 atggatgctc ttgaagaagc tgggtatcct agagatggta aattaacttt atatgatggt
     8341 agaacaggtg agtcttttga caacagaata actgttggat atatgtacta cttgaaacta
     8401 catcacttag tagatgaaaa actacatgca agaagtactg gaccatactc attagttact
     8461 caacagccac taggtggtaa agcacaattt ggtggacaaa gatttggaga gatggaggtt
     8521 tgggctctgg aagcatatgg agctgctcac atacttcaag aaatacttac tgttaagtct
     8581 gatgacgttg taggtcgtgt tagaacatat gaagctatag ttaaaggtga aaatatacct
     8641 gaaccaggaa tcccagaatc atttaaggtt cttataaaag aacttcaaag tttatgctta
     8701 gatgtaaaag tattgacaga tgaagaccaa gaaatagaag taagagaatc tgtggatgaa
     8761 gatgatacaa taggtgaatt tgagctagat gttgtaaatc atatgggaga agttgaagaa
     8821 agtaatataa tagaagaaat tgaagatgat tttgcagaaa atgcagaaga tgaggatata
     8881 gagaatttag aagaattcac tgaagatgat ttatttgaag aagaaataga ttttgatagt
     8941 gatgactttg atatgtaaat ttaattaatg taaatctcaa atagaaggga gagaactcct
     9001 tgtttgaatt aaacaatttc gagtcgataa aaatagcatt ggcttctcca gaaaaaataa
     9061 gacaatggtc taggggagaa gttaaaaagc cagaaactat aaattaccgt actttaaaac
     9121 cagaaaaaga tggtcttttc tgtgaaagaa tatttggacc acaaaaagac tgggagtgcc
     9181 actgtggtaa atatagaaga gttagatata aaggtgtagt ttgtgataga tgtggagtag
     9241 aagtaactaa atcaaaagta agaagagaga gaatgggaca tatagagcta gcggctccta
     9301 tgtctcacat ctggtacttc aaaggtatac caagtagaat gggactttta cttgatatgt
     9361 caccaagatc gttagaaaaa atattatact ttgcctcata tgtggtagtt gatccaggag
     9421 agactggatt aaatgaaaaa caattgctta cagaaaaaga atacagaact gctcttgaaa
     9481 agtatggata tacttttact gtaggaatgg gtgctgaagc tgtaaagaca ttactacaaa
     9541 atatagattt agagcaacaa agtaaagacc taagagcgga gttaaaagat agtacagggc
     9601 aaaagaaagt tagaacaata agaagattag aagttgtaga ggcatttaaa aagtctggaa
     9661 ataaaccaga atggatgatt ttagatgcaa taccagtaat accaccagat ttaagaccaa
     9721 tggtacaact tgatggtgga agatttgcga cttcagacct aaatgattta tatagaagag
     9781 ttataaatag aaacaataga cttaaaagat tattagagct tggagctcca gatataattg
     9841 taagaaatga aaaaagaatg cttcaagaag ctgttgatgc attgatagat aatggtagaa
     9901 gaggtagacc tgtaacagga cctggaaata gaccacttaa atctttatca gatatgttaa
     9961 aaggtaagca aggtcgtttc cgtcaaaact tacttggtaa gcgtgttgac tactcaggac
    10021 gttctgttat agttgttgga ccagaactta aattttatca atgtggactt ccaaagaaga
    10081 tggcattaga attattcaag ccatttgtta tggataaatt agttaaagaa ggttatgcac
    10141 ataatataaa aagtgcgaaa tctatagtag aaaaagttaa gccagaagtt tgggatgttt
    10201 tagaagatgt tataaaaagt catccagttc ttcttaaccg tgcaccgact ctacatagat
    10261 taggtataca agcatttgaa ccaatcttag ttgaaggtaa ggctataaaa ctacatcctc
    10321 ttgtatgtac agcttataat gcagactttg atggtgacca aatggcagtt catgtacctt
    10381 tatcagtaga agcacaagca gaagcaagat tcttaatgct ttctgtaaat aatatacttg
    10441 ctcctaaaga tggttcacct ataactactc catctcagga tatggtttta ggttgctatt
    10501 atctaacaat agaagcccaa gatggagcta aaggaacagg tatggtattt aaagacttta
    10561 atgaactatt acttgcttat tacaataaat cagttcatct acatgcatta gtgaaactta
    10621 aggtaacact tgaagatgga agaagttcat tggttgaaag tactgttggt agatttatat
    10681 ttaatgaaaa tatacctcaa gacttaggtt ttgtagatag aaaagaaaat ccatttgcac
    10741 ttgaagttga tttcttagca gataaaaaat ctcttggtaa aataatagat aaatgcttta
    10801 gaaaacacgg aaatacagaa acagctgaat tactagatta tataaaagct ctaggattta
    10861 aatattctac attaggtggc ataacagttg ctgttgatga tatgagtgtc ccagaagaaa
    10921 agaaagtatt tatagctgag gcagaagcta aagttgataa gtatgaaaaa gcatacagaa
    10981 gaggtctaat ctctgatgaa gaaagatatg aaaaagttat agagacatgg acagaaacaa
    11041 ctgataaagt tactgatgct cttatgggtg gactagatag attaaataat atatatataa
    11101 tggcacattc aggagccaga ggttctaaaa accaaattag acagctggca ggtatgcgtg
    11161 gtcttatggc aaatgcatct ggtaaaacag ttgagatacc agttaaatct aatttccgtg
    11221 aaggtttatc agtactagaa tactttacat cttcacatgg tgccagaaaa ggtcttgccg
    11281 atacagctat acgtacagct gaatctggat atttaacaag aagacttgtt gatgtaagtc
    11341 aagatgttat tgtaagggaa atagattgtg gaacagaaga tactacagag atttatgcta
    11401 taaaagaagg aaatgaagtt atagaagaga tatatgatag aattgtagga agatatacta
    11461 tagaccctat attaaatcct gaaactggag aagttatagt tgaggctgat tcaatgatac
    11521 aagaagatga agcagaaact atagtagctt taggaattga aaaaattagg ataagaacag
    11581 ttcttaactg taaaactaat catggagttt gttctaagtg ttatggtaga aacttggcaa
    11641 caggaaaaga agttaatata ggtgaagcag ttggtataat agcagctcaa tctatcggtg
    11701 agccgggtac tcagcttaca atgcgtacat tccatactgg aggagttgca ggagctgata
    11761 taactcaagg tctaccaagg gttgaagaat tatttgaagc tagaaaacca aaaggattag
    11821 ctgtaataac tgaagtatct ggtagagttg aaattgatga aacaggaaag agaaaagaag
    11881 taaatgtaat accagaagaa ggcgagactc aaacatatgt aattccatat ggttctagat
    11941 taaaagttaa gcaaggtcaa atgctagagg ctggagaccc tctaacacaa ggatttataa
    12001 atcctcatga tatagtaaga gtaaatggtg ttaagggagt tcaagaatat atagttaaag
    12061 aagttcaaag agtgtataga cttcaagggg ttgatgttaa tgataagcat atagaagtta
    12121 tagtaagaca aatgctatct aaagtaaaag ttgaagaccc aggagataca gatttattgc
    12181 caggtggata tgaagatgta ttaacattca atgaatgtaa taaagatgct atagataaag
    12241 gtctaagacc agcagttgct aaaagagttt tacttggtat aactaaagca tctcttgcaa
    12301 ctgattcatt cttatcagca gcttctttcc aagaaacaac aagagtatta acagaggcag
    12361 caataaaagg taaagaagac cacttaatag gacttaaaga aaatgttata ttaggtaagt
    12421 taataccagc aggaacagga atgaagaaat atagaaatat agctgttgaa aaaattgaag
    12481 attaatagca tatagagtaa agaaatcttg acaaccatta tttgtggtgt taaaatagta
    12541 aagtgtgttg tttaaaagta cactaagggc ttgaacaagt tcttagtgac ttttagatgg
    12601 caataaatga gaactaaaaa gcatcaagct agaaaagtgc taatatcaca tatttatgtg
    12661 ttatagacat taaattgtaa gtaacgaatg ttacaatcag tcagtatgaa tattcatatt
    12721 gatgtagttg gcggtcactg ataagatcga aaatttggaa ggaggtgcag atgatgccaa
    12781 caattaacca attagttcgt aaaagtagaa aagcgctaga gaagaaatct actgctccag
    12841 cattacaaaa aggttacaac tctttaaata agaaagtaac tgacgctagt gccccacaaa
    12901 aaagaggggt ttgtacttca gtaaaaacag ttactcctaa gaagcctaac tcagctttaa
    12961 gaaaagttgc cagagttaga ttaactaatg gtatagaagt ttctgcttat ataccaggag
    13021 aaggacacaa cttacaagag catagtgttg ttcttataag aggaggaaga gtaaaagacc
    13081 ttccaggggt tagataccat atactaagag gtactctaga tacagcaggt gttgataaga
    13141 gaagacaatc aagatctaaa tacggtgcga agagacctaa agaagctaaa aagtaaaaac
    13201 aattaatgat tgattatagt tcggtgcttt aaatatatat atatttacgg catcacggca
    13261 ttaaatgatt tatgtcgagt acctatgatt taagttaaat aattaaggag ggaagcgaaa
    13321 tgccaagaaa aggtaatatt ccaaaaagag aagttttacc agatcctatg tatgggagta
    13381 aggtagttac taaattaata aataatctaa tggtagacgg aaaaaaagga aagtctcaaa
    13441 gaatagttta tgatgctttc gcaattgttg ctgaaaagac aggagaagaa gctttagaag
    13501 tatttaataa agcaatggat aatataatgc ctgttttaga agtaaaagca agaagagttg
    13561 gtggggcaaa ctaccaagtg ccaatcgaag ttagacctga aagaagacaa acattaggtt
    13621 taagatggct agtaaaatat actagagctc gtggagaaaa aggaatggtt gaaaagctag
    13681 ctaaagagat aatggacgca gctaacaaca ctggagcttc agttaagaaa aaagaagata
    13741 ctcataaaat ggcagaagct aataaagcat ttgctcatta cagatggtag gatatataga
    13801 tatcttataa aattgaggat atcataagga ggataaccat ggctagaaag tttcctttgg
    13861 aaagaactag gaatatagga atcatggctc atatagatgc aggaaaaact actactacag
    13921 aaagaatcct gttttatact gggcaaactc ataaaatagg agaaactcat gaaggagctt
    13981 ctcaaatgga ctggatggag caagaaaagg aaagaggtat aacaataact tctgccgcta
    14041 ctactgcttc atggaaagac catagaataa acataataga tactcctgga cacgtggact
    14101 tcacagttga ggttgaaaga tctctaagag ttcttgacgg ttcagttgct gtattctgtg
    14161 caaaaggtgg ggttgagcct caatcagaaa atgtatggag acaagctgaa acttatggag
    14221 tacctagaat agcttttgta aataaaatgg atatattagg tgcagatttc tacaatgttg
    14281 taagcatgat gaaatctaga ttaaattcta atgcagttcc tatgcaatta ccaataggta
    14341 aagaagattc atttataggt ataatagact tattaaaaat ggatgctgtt atatacaaag
    14401 acgacctagg ggttgaaatg gaagaaacag atataccaga agatatgaaa gaattagctg
    14461 ctgagtggag agaaaaatta gttgaatcag tagctgaaac tgatgaagaa ttaatgatga
    14521 aataccttga aggtgaagaa cttactatag atgaattaaa agttgctata agaaaagcta
    14581 ctatagcttg tgaaatgaac ccagttttct gtggtacagc ttatagaaac aaaggtgttc
    14641 agttagtaat agatgcagtt ttagattact taccagcgcc aactgatata ccagctataa
    14701 aaggtatatt agcagatgga gaagaagctg aaagacattc ttctgatgaa gagccattct
    14761 ctgcattagc attcaaaata atgactgacc cattcgttgg aaaactagcg ttcttcagag
    14821 tttattctgg aacattagaa tcaggttctt atgttttaaa tgcaactaag aataagagag
    14881 aaagaattgg tcgtatatta caaatgcatg ctaatacaag agaagaaata actaaagtgt
    14941 atgctggaga tatagcagca gcagtaggat taaaagatac tactactgga gacacattat
    15001 gtgaccctgc taatccaata atacttgaat ctatggaatt ccctgagcca gttatatctg
    15061 ttgctataga accaagttct aaagcagctc aagaaaaaat gggtatagct cttcaaaaat
    15121 tagctgaaga agacccaact ttcacagtta agactgacca agaaacagga caaactataa
    15181 tatctggtat gggtgaatta cacttagaga taatagtaga tagattatta agagaattta
    15241 aagtagaagc taaagttggt gcaccacaag ttgcatatag agagactata actcaaccag
    15301 ttgatgttga atacaagtac tctaagcaat caggtggtag aggacaatac ggtcacgtta
    15361 agattagagt tgctcctcaa gagccaggtg aaggatacaa attcacaaac aaaactgttg
    15421 gtggatcagt accaaaagaa tatgttggtc cagttgacat gggtatacaa ggagctatgc
    15481 aatctggtat agttgctgga tatccagttg tagacgttgc tgttgagtta tatgatggtt
    15541 cttaccatga ggttgactct tctgagatgg cgtttaagat ggctggttca atggcattca
    15601 aagatgctat gaagaaaggt aatgcagttc ttttagagcc ttacttcaaa gtagaagttg
    15661 ttactccaga agattacatg ggagatgtta tgggtgactt aaactcaaga agaggattaa
    15721 tccaaggtat ggaagctaga agtggtgcac aagttataaa tgcattcgtt ccactttcag
    15781 aaatgtttgg atactcaaca gacctaagat cttcaactca aggtcgtgca acttacacaa
    15841 tgatattcga tcactatgag caagttccag cttcagttgc taagaaaata gctgaaggaa
    15901 aataatttaa aaataattaa taatataaat ataaaaaata atcagctaga tgagagatat
    15961 ctctcatct
//
LOCUS       DAEOSP010000076        15916 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.076, whole genome shotgun sequence.
ACCESSION   DAEOSP010000076 DAEOSP010000000
VERSION     DAEOSP010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15916
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.076"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            373..627
                     /locus_tag="KRH54_001939"
     CDS             373..627
                     /locus_tag="KRH54_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579597.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            663..986
                     /locus_tag="KRH54_001940"
     CDS             663..986
                     /locus_tag="KRH54_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579598.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIL
                     IDYII"
     gene            1260..1412
                     /locus_tag="KRH54_001941"
     CDS             1260..1412
                     /locus_tag="KRH54_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579599.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            1606..2529
                     /locus_tag="KRH54_001942"
     CDS             1606..2529
                     /locus_tag="KRH54_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBG8579600.1"
                     /translation="MVGAFVLPNYAIILSEMSNLWGNFIMWILVPIITIVLLIIAVSS
                     MQYILVMIAFLLIIYSFIEKKIVMGLVSVLFFTYSIYLCATWEDKSLIADNKVETVRA
                     QRETVEREKEMERRRIQEEVDKERYIEKHGMEISENDLKVKLEALVPQEYKGKKYELK
                     VGKFKRYSMYFDLTVQNEKFSNSEECKKFVKEIANDLKKIKISKAYFKFHSKDDGGIY
                     NSVYIDYFRNIQNNVDNVENLEFNEFELKTEEEEKREQEKIEQEKNSYNNYIQNRVVD
                     PLDRIKKLKELLDSGAITQEEYNKKKKELLE"
     repeat_region   2796..3810
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2796..2824
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            4001..4153
                     /locus_tag="KRH54_001943"
     CDS             4001..4153
                     /locus_tag="KRH54_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579601.1"
                     /translation="MKNRRKDKRGFSVLLDKEKLDKFDEVLEEKNLTKKEWLEEKIDE
                     ELEQKE"
     gene            4274..5087
                     /locus_tag="KRH54_001944"
                     /pseudo
     CDS             4274..5087
                     /locus_tag="KRH54_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888845.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            5139..5300
                     /locus_tag="KRH54_001945"
     CDS             5139..5300
                     /locus_tag="KRH54_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579602.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(5629..6093)
                     /locus_tag="KRH54_001946"
     CDS             complement(5629..6093)
                     /locus_tag="KRH54_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579603.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKKKILRERKYKKRR"
     gene            complement(6229..6546)
                     /locus_tag="KRH54_001947"
     CDS             complement(6229..6546)
                     /locus_tag="KRH54_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579604.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITILAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(6924..7124)
                     /locus_tag="KRH54_001948"
     CDS             complement(6924..7124)
                     /locus_tag="KRH54_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579605.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILEDIEIDLI
                     IIDEKNNVVWRNEKNTPAHENA"
     gene            complement(7143..7556)
                     /locus_tag="KRH54_001949"
     CDS             complement(7143..7556)
                     /locus_tag="KRH54_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579606.1"
                     /translation="MKVKSKRSFIVGIIVCMLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSADERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(7540..7773)
                     /locus_tag="KRH54_001950"
     CDS             complement(7540..7773)
                     /locus_tag="KRH54_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8579607.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            7992..8366
                     /locus_tag="KRH54_001951"
     CDS             7992..8366
                     /locus_tag="KRH54_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8579608.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEEYFKNLK"
     gene            8978..9670
                     /locus_tag="KRH54_001952"
     CDS             8978..9670
                     /locus_tag="KRH54_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8579609.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            9866..11926
                     /locus_tag="KRH54_001953"
     CDS             9866..11926
                     /locus_tag="KRH54_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG8579610.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(12132..12455)
                     /locus_tag="KRH54_001954"
     CDS             complement(12132..12455)
                     /locus_tag="KRH54_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBG8579611.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(12448..13149)
                     /locus_tag="KRH54_001955"
     CDS             complement(12448..13149)
                     /locus_tag="KRH54_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBG8579612.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(13427..14812)
                     /locus_tag="KRH54_001956"
     CDS             complement(13427..14812)
                     /locus_tag="KRH54_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG8579613.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIGFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(14920..>15916)
                     /locus_tag="KRH54_001957"
     CDS             complement(14920..>15916)
                     /locus_tag="KRH54_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG8579614.1"
                     /translation="ITLDIFMYIWILGIIAISFYYLRTNFKFYNYIENFKKEILEIDI
                     TSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTRCKIIIPEKKYNNKELRFIFKHEL
                     IHYKRKDNLFKLLISIICIIYWFNPILYLLKKYFHELCELSCDEIVIDKCTTSEIKEY
                     AYIILDTIKYQKSLKYSSFVSQFLNKKNIILKKRLNSMFNQDKKKINLGLKFLLTFII
                     IGSIFSINSNFKQNEDVEYTIGKINYNKNGETYGTHIIDSNGNSIEPELIQTIGRGKK
                     VGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFKKTIPLYDKEGIKIIGRFEIN"
BASE COUNT         6261 a         1730 c         2389 g         5536 t
ORIGIN      
        1 ttagtatgca aaattctgtg aagcaggaaa cctgtgactt tagtcatggg aggttcaggc
       61 tagtattact cattattgta ttcaaaagct aaagtggaaa cctagtgaat atatgaattt
      121 agaagttaat gagagagcat tagcagccga ctcaatactt attaaaattg aagatgaaga
      181 agaaacaatg aaagaagctg aaagagagaa aaagagaggt agaagaaggt agaatttaaa
      241 gttaaaatag ttacattgct aatatatgaa aatgttacaa atttggaaat agatggatat
      301 atatgttgta tagtataatg tagataatat atcaccaagt aaaaaacaac aaataaatct
      361 aaattatgag gggtggaaaa taaagatatt aaacttacaa tatgtatatt tgcttttgta
      421 attggaataa tagtgttatt ttgctgtata ggtctaggtc aatttaatat ggattgtatt
      481 ataaaacaaa atggtgggtc aatagctacc gaacaataca acatatattt agaacaatct
      541 atttcacaat atagaaattt tggttctata ctggttttac taggaggaat aggaatactg
      601 tttgataaaa gtgtcagaaa atactaaaaa agtatatttt tatttaggat gtgatgaatg
      661 atatggcaag agatgtattt agtgtaaaga tgacaccact agaagcacag aaaatggtag
      721 aaaaaaattt ggaagcagat ttagtttctg ttgatattta taacctagaa aataataagt
      781 ctataatagt tacaatcttt gaaaaatatt atgcaagaag caatagtgat gcaggtttgg
      841 tagtgatttg tgaaaatact acaggaaaaa cacttgttaa aattacctca actggtagtg
      901 caattggact atttcagatt gattggggaa caggtaataa tttgattaaa agagtaaaaa
      961 aaatacttat tgattatatt atttaattat tttatgtaga tattcttagg ttggtatgga
     1021 atttgtacta acctattaag taatcaataa aactaaataa acatagaaag cacttacaaa
     1081 tatgtaggtg ctttttattg aaaaaataga aaataaaata aaaagtaaga tataggaaaa
     1141 atatgtaaga attatatgtt ataataattg tagcaagaag atgtaatcta caatttatag
     1201 agtggagttc atgtattaaa aaattatcct cccaacgata agaagggagg tgaatataca
     1261 tggataattt tttatttaat gtgttagcta gtgtaacagc tagttacata gtttatttga
     1321 ttagcaagtt aatcaaaaaa gtaaaaagcc actctaccgc aaagagtgac ttaaagattg
     1381 atataaattt taaatatcat cgtaaaaaat aactttcagt tatagaactt cactctaccg
     1441 caaatagatt gtagttcttc ttgtttttat tataccacaa attagaaaaa atattgcata
     1501 taatattttt tatagtcaat aaaaatatga aattttttat acacaatgat gatgaatcta
     1561 actagatgaa taaatttaat tgtaaataag aaagtactta cttaaatggt aggtgctttt
     1621 gttttgccaa attatgctat aatattgagt gagatgagta atttgtgggg gaattttata
     1681 atgtggatat tagtaccaat aataacaata gtattactta taatagctgt ttcttctatg
     1741 caatatattt tagttatgat agcttttttg ttgattatat attcttttat agaaaagaaa
     1801 atagttatgg gtcttgtgtc agttcttttt ttcacttatt caatttatct ttgtgcaact
     1861 tgggaagata aatccttgat agcagataat aaagttgaga ctgttagggc acaaagagaa
     1921 actgtagaaa gagaaaaaga aatggaaaga agaagaatac aagaagaagt agacaaagaa
     1981 agatatattg aaaaacatgg aatggaaata tcagaaaatg atttaaaagt taagctagag
     2041 gctttagtac ctcaagaata taaggggaag aaatatgaat taaaagtagg aaaatttaaa
     2101 agatatagta tgtattttga tttaactgta caaaatgaaa aatttagtaa ttctgaagaa
     2161 tgtaaaaaat ttgtaaaaga aattgctaat gatcttaaaa aaattaaaat aagcaaggcg
     2221 tactttaagt ttcattcaaa agatgacggc ggtatatata attctgttta tattgattat
     2281 tttagaaaca tacaaaataa tgtagataat gttgaaaatt tagaattcaa tgagtttgaa
     2341 cttaaaaccg aagaagaaga gaagcgagaa caagagaaaa tagaacaaga aaaaaacagt
     2401 tataataact acattcaaaa cagagtggtt gacccactag atagaataaa gaaactaaaa
     2461 gagttgctag attcaggagc aattacacag gaagaatata ataaaaagaa aaaagaatta
     2521 ttagaataga taatatagta agcacttaca agtatgtagg tgcttttaaa tttacaaagt
     2581 attccatttt aattttatag tttagatttt atgatataat aaaaatatag aagttttgca
     2641 gtgtgcgata tttgttacaa agtagggctt aatacttgaa atctaagatg ttgagggtgc
     2701 gtgataagtg ttatcaattg cactattgcc cgctcactgc aattttaaga gtattgtata
     2761 tatgtaggta ttggaaatgc taagtttatt ttggggtttt agattaacta tatggaatgt
     2821 aaatattaca tgggtaaaaa ttttgatgat ttttatgaca gttttagatt aactatatgg
     2881 aatgtaaatt gtatagaatt aagaattttt ttaatactaa ttatagtttt agattaacta
     2941 tatggaatgt aaatattttt caatatcaat atagaacttt catctttccc gttttagatt
     3001 aactatatgg aatgtaaatc aagatatttt taataaaatt tctgagcgaa ctgtttgttt
     3061 tagattaact atatggaatg taaatgagga gattcagaca gcgactaaaa agactgatat
     3121 aaagttttag attaactata tggaatgtaa ataactttat atctccattt gcatatccat
     3181 ctgatgaacg ttttagatta actatatgga atgtaaatgc gtacaaggtt gagaagctcc
     3241 gcaatctcaa cgtcgtgttt tagattaact atatggaatg taaatatttg aaaggaataa
     3301 attttaaatg aatgaaagga taggttttag attaactata tggaatgtaa atcagccaaa
     3361 aacttcgaat ttgaactaaa atgcgtgggt tttagattaa ctatatggaa tgtaaattct
     3421 catgactatt gaataaatat aataaacaag catacgtttt agattaacta tatggaatgt
     3481 aaatttagaa tcaaaagcac ttaatcctag ttctttcata gttttagatt aactatatgg
     3541 aatgtaaata gctttttagc ttcgtctact actttataag caaagcgttt tagattaact
     3601 atatggaatg taaatgatga acaataaaac aatcatctaa agacgaagaa agttttagat
     3661 taactatatg gaatgtaaat gttcctgttg tgttttttac taatcccatt ttatatgttt
     3721 tagattaact atatggaatg taaattgctg tgatttcact aataaaacaa ttaataattg
     3781 tgttttagat taattatgtg gattcaaaat taaataaaca aagaaagcac ttacaaatat
     3841 gtaggtgctt ttattctgtt caaaattggt cggttgggta aaataattag aaaaaattag
     3901 taaaaacctc ttttctgtaa ctcgttacaa tattattatt aatgtaacga gttacagaaa
     3961 agaggtgaat aaaatagcaa ctaaaagtag ggcagagtat atgaaaaatc gtcgaaaaga
     4021 taaaagaggt tttagtgtac ttttagacaa agaaaagtta gataaatttg atgaagtatt
     4081 agaggaaaag aatctaacca agaaagaatg gctagaagaa aaaatcgacg aggaactgga
     4141 acaaaaggaa taaaaaataa gggtcactcc caccgaccaa agtttgagta acccctatga
     4201 cgtatactat catatatcaa ttatagtata tgtcattcct taaaaaaaat caattattaa
     4261 ggagtgtaat attatgaaaa atgaattaat gatgtttgaa ggaaaagaga ttgaagtatt
     4321 tgaatttgaa ggaagaattt tattcaactc aaaagatgta gcaaactgtt tagacataaa
     4381 aaatgttaat gagaatataa gacttatgaa taaaaagcaa gttgtaaagt taagaaattc
     4441 ggatatcagt aatactgaca tccgaaaact taataatgct ggagaaaatt ttcttactga
     4501 aagtggagtg tataaattaa tctttaaatc aagaaaagaa gaagctgaga gattccaaga
     4561 ctggataagt gatgaagttt taccaagcat aagacaaact ggtgcataca taactaataa
     4621 tgcaatcccg aaaagttgag agaaaaagca agtgagattg aaaaattaca gttgcttata
     4681 acagtacatc tatgttaaaa gaattgttag atgatgcagg ctttgacaat aaatcaaaac
     4741 tattaacagc taaaacatta tataagaaag caggaattga tttaccaata gagataaacg
     4801 aagaagaaca ttattttgat acaaaacaaa tagcatctaa actgaaaata tattctaaga
     4861 gtaataaacc agctcagatg gctgtttgtg agattattaa aaagattgat ttagaagatg
     4921 gtgaagttaa aggtgtttgg taaactaatg gttcttggac tggtactgta aataaatata
     4981 caaagagtgt aatagataag gtgagaactt ggatagagga aaataataga cctaccaaga
     5041 ttgcaggtga gaagaagaat tatcatgtat cttacaaaat tgagtaaatt tatcagttgt
     5101 attaaataat ttagtttagt tttgggggga ttaatacaat gtgtgagaat ttacttgata
     5161 atatgtatat tgaaaagaga aaagaagaat atagaattaa acttttgaaa ataagagaaa
     5221 cagatataga tatatataat aagttagaaa gtatagtata taaactttct gagaaaaaat
     5281 tagagaaaaa taataaatag acatataaag cacttggata ttctactgtt tcaagtgctt
     5341 tgtttggtat aaaatggtat aatggagata gagaattata ttaacccaaa aatggagaaa
     5401 atagagaaga tgatttagtt gacgctttat cagtaggagt acttgcaggt aagacagaat
     5461 ctctagtact attagttgga aatggaataa gtgatactca aaaaagttta ataaataaaa
     5521 gagaatttag aaatataact caaattggtg gaaatggaaa tgagaaagca tttagtgaag
     5581 tagaaaactt agttaaatag attagaaggt agcaactaga gttggttgtt accttctttt
     5641 tttatacttt ctctctctaa gtattttctt tttcatatct tctaatacat ctaatgcagt
     5701 agtataaaat tgtatattat ttctataagg tatagctatt ggaagaatgc ccttgttaac
     5761 taaaaaaata agaagtacaa taaataaaag tgtcataatc attctaataa aataattagt
     5821 gatatctgat aaatcaaatg ttaagttaat tgttgtagaa aatacaacag ccataataga
     5881 tatatacaaa gcaaaaatgg ggttactatt tttttgtaac tgtcctttaa gcctagattg
     5941 ctctatttct atatcttgaa tactttttat ggaatccatt tcactataat acttataaac
     6001 ttttaaataa tattttttat acttgtttaa agcttcttca ttatctttaa ggtctttgtc
     6061 acttaactct gatttaactt ccccaaaatc cataaaatct ctcctacaat gttatatagt
     6121 atactttaat ttatagcata tttaagaact tatatcagca atttgtcgaa caattttagc
     6181 taaaaataga gtataatgat aggcttatta tttataagat attatttttt atatagttat
     6241 atatttcttt ctaattagat aatataatgc tgttgttata atggctacta atattattgc
     6301 tagtattgta atatctaatc ttcttgtaaa taagcctatt gctatatact caagacctgt
     6361 tacataacga taaagtactt ctagtttaac agtttttttc ttatctatat ttccatatat
     6421 tgattctaat ttattaaaat attttttact acaaaaagca gttttaggtt taattaaata
     6481 tattattgta gttcctatta taactattat tccaataagt ataagattga tatttccaaa
     6541 atccatagca gatacctcct attaaatttg cttttcttag tcaaagtata tcagtaacac
     6601 aagtacaaag agtatataaa atattacaaa tattaacagg atagataaaa ctatagtaaa
     6661 aattgtaata ataaagcgaa cgaaaattat catactaaaa cataagacat gttataattg
     6721 tattagatag aagattaaca tacaccaaag cactccttat aataaagagt gcttattttt
     6781 ttgaaattca tcaatatata aactatcaag aatcttattc aacacacctt aaaatcgatt
     6841 taaatagctc tttttgatag cataatttat atgatataat aaaaaaatag aagtgtattc
     6901 gcagtacact tctatagtta taactaagca ttctcatgag cgggagtgtt tttttcattt
     6961 ctccaaacaa cattattttt ttcatcaata attatcaaat caatttcaat atcttctaaa
     7021 atttctgttg cagtacaaat tagattatct aagtcaaaac ttcgagttat ctctagttgt
     7081 ttgttattta cttctctttt tagtatataa gttttaaaat acatttatat caccccttgt
     7141 atttactctt ttttctctaa aaatatattg ataagtaaat atgcaacaaa taaaaaaatc
     7201 tcaatgacac atagtgttat agctataact agaagtgatt gatttttcca tgcactataa
     7261 gctatgatga aaagaaatgt aaaagtaaat aatgtataat tcataatttt aactaagata
     7321 tgacttgttt tcattgttaa gtacaagtct ctttcatctg cactatcgtg taattcttca
     7381 acaatgcctt ttcttgaaaa agcattacaa aaattaaaga ttgctattac aattaaaaga
     7441 aatgatgaaa ttaggaatct tttatctttt aaaatgcaat aaataactaa actagcacaa
     7501 cataacatac aaactataat acccactata aaacttcgtt tactctttac tttcatgttt
     7561 tttatcctcc atttctttat tttcttttaa acaacatagt tcttcaatcg ttgtaccaaa
     7621 cacttgtgcc atacgatagg caagcataag agaagggcta tattgctctt tttcaataga
     7681 aataattgtt ctgtttgata catggactaa atcagcgagt tgttgctgtg ttagacccat
     7741 atttgttcga aattctcgta cttttgtttt cattccataa cctctttcta tatgaagcta
     7801 acttcatgtg aagttaactt cataatatcc taaaatgagt aaaatgtcaa tctcattaga
     7861 taagtattat aaaacctaaa agaataatat ggtaaaaatt ggatggaatt atttacctta
     7921 attctcttgt ttgtaaaact ttattgatat agaatttaac atatacataa atcggcaaag
     7981 gggtgcgaat tatgaaaatt agtaaattac cagaagcgga attaaaagtt atgagatata
     8041 tttgtgaagc taacaaggta ttaacttcta gggaattagt tgctgcaatg gaagaaaaat
     8101 atgagtggaa tgaatcaact acatttacag ttttgaaaag attagagcga agggagtttt
     8161 taagtacaga gaaaataggc aaacttacac attataatat actagtaaaa gagaaaaaat
     8221 atctaaggtt tgaaacaaag gaatttttga agaacataca taaaaattca atttcaagtt
     8281 taatgtcagc gttacatggt gaagacgacg aagtggatga agataaatta agtgaactcg
     8341 aagaatattt taagaatttg aaataagaaa atgacaaatt gatacataat aaataaaatg
     8401 aaggtatgaa attataactt tttctacctt cattttgttt attattctga acatgttaag
     8461 aaaatgaaat aagtttgtat tttagttata taaattttaa tagaatatgt acagtatata
     8521 gtagatagaa tgtataaaaa tagaatttaa ataagtagct attattgcag tgaaatgaat
     8581 gtttctaagt atatttgggt ggaagaatgg tggaagaaag ttagtgaaat gtaattcttc
     8641 caccattctt ccacccaaaa aataaaaact gttcaataag aattaatata catcaattca
     8701 gctgaatgca atcaaaatac ttaaatgaaa gtaatctgaa aacaacataa ttcaaatcaa
     8761 tttattcaaa taaacctcaa tttatcttaa atcttaagaa atatttaaga attaggataa
     8821 tattttctta agatttatat tttattatat agttataagg gaaagttaca aaaatgtaag
     8881 ccaaagtaat ataacaattt agtcattgtt gagaaaggga gtgaaagata ttgtataata
     8941 tagataagta ttattataca atagaggaag tgaaaatatg tacaatatat tagtagtaga
     9001 cgatgataaa gaaattgttg attcgataga aatatatctt aaatcagaag gatttaaaat
     9061 atataaagca tatgatggaa tgaaagcttt ggaaataata acagagaatg acattcatct
     9121 aatactaatg gatataatga tgcctaaatt agatggaatc aaagcaactg taaaaatacg
     9181 agaagaaaga aatattccaa taattttaat ttcagcaaaa agtgaagata cagataaaat
     9241 aataggatta aatattggag ctgatgatta tataacaaag ccatttaatc tattagagct
     9301 tatagctagg gttaaatcaa acttaagacg atatgtaact ttaggtaatt atgaagatac
     9361 atgtaaagaa attttaaaaa gtggagcttt ggagttaaat acactaacaa aagaagttaa
     9421 aattgatggt gaaaacataa aaatgactcc aattgagtat aaaataattg aacttctttt
     9481 agaaaataaa ggacgagttt tctcaataga tgagatatac gaaaaagtat ggaatgagga
     9541 aagttttaat tcagaaaata cagtagcagt acatatcaga cgaataagag aaaagattga
     9601 aataaatcca aaagaaccaa gatttttaaa ggtggtgtgg ggagttggtt ataagattga
     9661 aaaattttag tggaaaaatt ataaaagaaa agagtttaat atttaattgc attttgattt
     9721 tagtcatttt tacatttaca caaatgttag gaattttaat atgttttaat tttttctctg
     9781 agagtttaat ttactctatg gggattatgc ttttcttaaa ctttatttta tataaaatac
     9841 tgacaatgta gatgaggtgg gcaacttgga gaaatataga gtattaaagt tagtttcatt
     9901 aatcataata tctatctcat ttccattaac tatattgttg gtgacaatag gacttgctga
     9961 gcctaaatac aatatacaaa aaacttctac aaagagtggg atttttgata tatctgatga
    10021 tttttatgat agtaatatat ttgacgaagg aacaatggca agtctattac ccaagtttaa
    10081 gctatctgta ggtggtaaat taactagtaa agaaaaaggt aaattccaac aagaaggtaa
    10141 aagtatagat ttaagtagta gtgattctga gtatgataaa atcatgatta atgaacaagc
    10201 ttctgaagaa tatttaagtt atctaaaaaa tataaaagtg tatgttatta atacatacac
    10261 aggagtattt tatacaaata cgtcatatgc tagtttatat gattttaaac aaaaaactaa
    10321 gagctattgt aatgtagaga taataaataa gataggaaag agctcatata ttaaaacaat
    10381 aaatggagaa aaatttgaac tgaataatct gagtctggaa ctaaatgaag tagatgaagg
    10441 ttttgaggca tatgtatctt ttccagaaga gccaactata gaagatggaa tagtatatac
    10501 taattttcaa atatttaaac aagctacaga aaaagtaaga ttattttttg tagctagttt
    10561 aagtttagca attatatgtt tattatctat aattttatat atcaaaataa aaggtgaagt
    10621 tttaaaaaga aataatgtat ttttactaat atacagtaag atacctatgg aatttaatgt
    10681 aatattagta tctatttcaa ttgcaatttt aactatatat gctcttgctg tatcttatca
    10741 tattgatgta attgcattag tttctttatc tttactgata gtatcaaata atatattttt
    10801 tataaatgtg caattgagtt atttagaaaa taaaatgtgt atatttaaga caagtattat
    10861 agtaagagtg attttatggt taataaaaac tgtaagggag gtcagtaacg ctataaagaa
    10921 agtatcttta gcaaggaaag ttattgttat agtgataata tctttagctt taatcatggt
    10981 aattagcact cgaagtatag gtatgggatt actgttttct atatgtattt ttttaggatt
    11041 tacaatatat ataactaaaa gactttcata tcttagttac gtaatagaag gtacagaaag
    11101 aataaaaaag ggagaattag attataagat aaaaattact gggaatgata attttacgtc
    11161 tttagctgaa aatataaata atattggaga aggtcttgat agagctatag aagagcaagt
    11221 aaaaagtgaa agaatgaagt ctgaccttat aacaaatgta tctcatgatt tgaaaactcc
    11281 acttacttct ataataaatt atgtagattt aattaaaaaa gaagaatcaa tacaaccaga
    11341 gtatataaat gattatataa atgtacttga gtcaaagtca aaaagactaa aattattgat
    11401 agaagattta tttgaagcga gtagagtaag tagtggcaat atagaattac aaattagcaa
    11461 gattgattta gtgcaactat taagacaaag tataggtgaa cttgaagaaa aattatctaa
    11521 aaataattta tatttaaaat taaatgttcc aaatgataag gtgtatatat gggcagatgg
    11581 aagaagaatg tatagagtat ttgaaaatct tttatctaat atagcaaaat attccttaga
    11641 agaaactaga gtctatattg atgtatatga ctatggtgaa aatgtaaagg taactatgaa
    11701 aaatatatct tcttatgaat taaattttga cccaagtgag attatggaaa gatttaaaag
    11761 agctgatgaa tcaaggaata ctgaaggaag tggattaggg cttgctattg caagagattt
    11821 agtagcttta caaggtggaa aattttatat agaaatagat ggagatttat ttaaagcaaa
    11881 tttagagttt caaaaatatg aagaagaaat aacaaatatg gaatgagata gtatttaagt
    11941 atatatattt tttttactgt cgaaagcttt tattataata tattagaata tttacagtag
    12001 agaatgaaga gtaaaaacag agtaacttta agtcactctg ttttttattt aaaatcaata
    12061 atataacaac ttaaattaaa attgtacaca tagttttaaa ataacttata caataaaaca
    12121 ttatccaaat atcacaagat aattccaaat aaatatatag atatcactcc tgtaataata
    12181 gaaataccaa tagatttaaa cttatatgca acaagaagta caatcataaa agatataatt
    12241 ttagggttag ctatagataa atatagacta tctccattaa aaaatacatc tttagcagtt
    12301 aaggaagtaa gtattgctac aggtataaat ttcatgattt ttttcaaaaa atcaggcatc
    12361 tctttttttg aaaaaattaa cataggaagc actcttggta gatatgtaac aatgtacatt
    12421 ccaattataa taaaaaatat ataattatta ttcataagat tcctcctttt tacagtaaga
    12481 ttcttctata aagaaaccaa ttaaagatgc taaaattgat gcaactatta ttactaaatt
    12541 gcttttgaat aatgcatata aaataacaga aataatgatt gatgatataa atactacaat
    12601 ataaatctta ttttttattt gagaaactat taaagctata aacatagaag tcaatatgaa
    12661 gccagaaata gaactgtcta tagatatact ttctcctaaa aatgctccaa taaaattagc
    12721 taaaactgat gaaattaagc agaagaaatt aagataaata gcatttctat cagaccaatt
    12781 tccagcagta aatttttcat aattaactgc aaaagtttca tctgttatag tatgacaaaa
    12841 tggcaatata aattttggac ttttattttt tatgtacatg ttcaaattag aactcattag
    12901 aaaatgtctt aagttaacta taaatgtagt caaaacaata gatgttacac ttgcaccagt
    12961 agaaatcatt gaagctgcaa tgaattgaga acttcctcca aatactaaag ttaccattag
    13021 tgctatttga aaaggtgaaa gaccagattt ttgtaatagt attcctccta ctatacctat
    13081 aggtatataa cctaagaata taggaattga agcaataaag ctttctttta attgttgagt
    13141 tctttccaca ataaatcact ccttttgtat taaatgtatt aaagtattcg taaaacagaa
    13201 aaatagatta attgtataat tgcaattata attaaaaata aatttggatt aatatataaa
    13261 atactataca acaaatagat aatcttaaac ggttattaaa ttttatctat ttgttattat
    13321 gatgtaataa tacatattaa tctctataag ttaattacta tctggtgtaa ttttatttta
    13381 acaacaaaaa aaaatacaca caattaaaat attgtgtgca atacaattat tttttctctt
    13441 ctaatagtct ttttaatacg ctcaaaccag ctttaagttc ttctgtactt tctgtagcac
    13501 atacagctat tcttattgca gaaattggtt tttcttttcc aacagcaaaa cgttcagaag
    13561 catagacctg tacaccttct ttaagtgctt gtatctcaaa tttttctgcc attattccat
    13621 caggtagaat caaccatcta aatatacatt cttcatttcc caaaagatta tatccagata
    13681 aatattgatt aataatcttg tttcttcttc ttgcagattt acggcgtgca ttaattaatt
    13741 tatcggcttc acctgatgta atcatgcgat aagcaatttc tgttaaaaaa taagattgag
    13801 atagattaat gttgtataat gcgcttgata atgcctctcg atactgttta ggtgtagaga
    13861 tatatgctag ccgtaaacta ggtgcaataa tttttgataa actcgtaata taaattgttt
    13921 gattgggcag ataggaagcc actggattta aatgtgtttc attaagtaaa ctatgaatag
    13981 catcctcaat aacaattaaa ttgtatttag atgcaatatt tgcaatcatt tttcgtccat
    14041 tttgtgacat tatatgtgta gttggatttt gataatctgg tattatgtat agacctttaa
    14101 tattttcatt tttacaagca tataatagtc cttcttcact aatctcatta tcttcttgtt
    14161 taattggaat tagttgaatc ccaagcattt tagctgctgt ctttattcca ggataagtta
    14221 atgggtctac acctattcta tctccatgtt gaaacaaacc agctaaaata gcaacaattg
    14281 cattttgtcc accacttgca ggaagtaagc tatctgatgt agtttcaagc ccagctttat
    14341 aaattaattt ggcaatagct tctttttgcc atttagcacc atctctttgt ccatattgga
    14401 atatggtttt aaaacctatt tccttaatta tgtgtttaaa caaagtattc atttcttcta
    14461 gcgtgaaatc tggattcatt gtgcccattt caataaatgt aattttatta ttactggaca
    14521 tcaagaatag atttgaacgt gtatcataag atacaaaagt tccacttcct gtaactccac
    14581 ttattagacc ttttttttca caaattttaa aagctctaga gatagtactc acattaacat
    14641 ctaagaaatc tgccaattca cgttggggtg gtaatttagt acctggtaat aaaaatccat
    14701 tgttgatatc ttgttctagt tgttctgcaa gcgccttata taatatttgc ccttttgaag
    14761 gtctctttgg tttccagttc ataggataat tttcaaaaga attaattggc ataagttttc
    14821 acctcacatt attaatttga ttttttcttt atctaaaaca ataaattcta aagatacaaa
    14881 taaaaactga taggaaataa ttctatcagt ttttgtcttt taattgattt caaatcttcc
    14941 tataattttt ataccttctt tatcataaag agggattgtt tttttaaata tattaaaatt
    15001 tctttttctt gtatataaaa cagcttcttc tggtgtcttt ggttgattat cacaatcgta
    15061 caaatctctt ttttttacgt agccgacttt tttacctcta cctattgttt gtataagttc
    15121 tggttcaatg gaatttccat ttgagtctat tatatgtgta ccataggttt ctccattttt
    15181 attataatta atcttaccaa tagtatattc tacatcttca ttttgtttaa aattggagtt
    15241 gatagaaaat attgacccta ttattataaa ggttaataaa aacttaagac ctaaattaat
    15301 tttcttttta tcttgattaa acatagaatt taacctcttt tttaaaataa tatttttttt
    15361 atttagaaac tgagatacga atgatgaata cttcaaagat ttttgatact ttattgtatc
    15421 taatattata tatgcatact ctttaatttc actcgtggta cacttgtcaa tcacaatttc
    15481 atcacaagat aattcacata attcatgaaa atattttttt aataagtata aaattggatt
    15541 gaaccaatat atgatacaaa ttatactaat tagaagctta aataagttat cttttctttt
    15601 ataatgaatt aattcatgtt taaagatgaa tcttaactct ttattattgt actttttttc
    15661 tggaattatt attttacagc gtgttaaacc tataatggca ggagaataaa tacctttaac
    15721 cttatatatc tgaagttttt tatttatact tagttctttt gcttgttcat ctaagatact
    15781 agtaatatct atttctaaaa tttctttttt aaaattttct atgtaattat aaaatttaaa
    15841 atttgttctt aaatagtaaa aagagattgc tataattcct aatatccaga tgtacataaa
    15901 tatatctaaa gtaatg
//
LOCUS       DAEOSP010000077        15847 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.077, whole genome shotgun sequence.
ACCESSION   DAEOSP010000077 DAEOSP010000000
VERSION     DAEOSP010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15847
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.077"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            216..770
                     /gene="tcdR"
                     /locus_tag="KRH54_001958"
     CDS             216..770
                     /gene="tcdR"
                     /locus_tag="KRH54_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBG8579615.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSVFEEDICFFEYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            1088..8188
                     /gene="tcdB"
                     /locus_tag="KRH54_001959"
                     /gene_synonym="toxB"
     CDS             1088..8188
                     /gene="tcdB"
                     /locus_tag="KRH54_001959"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBG8579616.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGIASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMLSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDDKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8310..8810
                     /gene="tcdE"
                     /locus_tag="KRH54_001960"
     CDS             8310..8810
                     /gene="tcdE"
                     /locus_tag="KRH54_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBG8579617.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            9139..9321
                     /locus_tag="KRH54_001961"
     CDS             9139..9321
                     /locus_tag="KRH54_001961"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579618.1"
                     /translation="MRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            9538..>15847
                     /gene="tcdA"
                     /locus_tag="KRH54_001962"
                     /gene_synonym="toxA"
     CDS             9538..>15847
                     /gene="tcdA"
                     /locus_tag="KRH54_001962"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021414120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBG8579619.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQT"
BASE COUNT         6404 a         1652 c         2481 g         5310 t
ORIGIN      
        1 acaatacttt attaatataa agtttattgc taaaatactt tatttattag aaaaagatta
       61 ctaattaatt attaaaatta atgtattcat aatgcatatt ttcatataaa atttaattta
      121 tttgccgatt atataattat aatgactgat ttaattccaa tgttgtcaaa attttcgaat
      181 aaatcatcat tataaatata agagaggatg attttatgca aaagtctttt tatgaattaa
      241 ttgttttagc aagaaataac tcagtagatg atttgcaaga aattttattt atgtttaagc
      301 cattagtaaa aaaacttagt agagttttac attatgaaga gggagaaaca gatttaataa
      361 tattttttat tgaattaata aaaaatatta aattaagtag cttttcagaa aaaagcgatg
      421 ctattatagt caaatatatt cataaatcat tactgaataa gacttttgag ttgtctagaa
      481 gatattctaa aatgaagttt aattttgtag aatttgatga aaatatctta aatatgaaaa
      541 ataattatca aagtaagtct gtttttgagg aagatatttg ttttttcgaa tatattttga
      601 aagaattatc tggtattcaa agaaaagtta ttttttataa atatttaaaa ggatattctg
      661 atagagaaat atcagtgaaa ttaaaaatat ctagacaagc tgttaataag gctaaaaata
      721 gagcatttaa aaaaataaaa aaagactatg aaaattattt taacttgtaa ttaatgagct
      781 taaagaaata tttacaatag aaatcaaatt ttagaattaa ctttattgta aaatcaataa
      841 cttaatctaa gaatatctta atttttatat tttatataga acaaagttta catatttatt
      901 tcagacaacg tctttattca atcgaagagc aaattaatca actgagtgtc ttcaatttaa
      961 aatgttagga agtgaatgta tatgaaaacc taagtagata ttagtatatt ttataaatag
     1021 aaaggaggat atataaaaga gttttatcat ttagatgtaa aaatattata gtaaaggaga
     1081 aaattttatg agtttagtta atagaaaaca gttagaaaaa atggcaaatg taagatttcg
     1141 tactcaagaa gatgaatatg ttgcaatatt ggatgcttta gaagaatatc ataatatgtc
     1201 agagaatact gtagtcgaaa aatatttaaa attaaaagat ataaatagtt taacagatat
     1261 ttatatagat acatataaaa aatctggtag aaataaagcc ttaaaaaaat ttaaggaata
     1321 tctagttaca gaagtattag agctaaagaa taataattta actccagttg agaaaaattt
     1381 acattttgtt tggattggag gtcaaataaa tgacactgct attaattata taaatcaatg
     1441 gaaagatgta aatagtgatt ataatgttaa tgttttttat gatagtaatg catttttgat
     1501 aaacacattg aaaaaaactg tagtagaatc agcaataaat gatacacttg aatcatttag
     1561 agaaaactta aatgacccta gatttgacta taataaattc ttcagaaaac gtatggaaat
     1621 aatttatgat aaacagaaaa atttcataaa ctactataaa gctcaaagag aagaaaatcc
     1681 tgaacttata attgatgata ttgtaaagac atatctttca aatgagtatt caaaggagat
     1741 agatgaactt aatacctata ttgaagaatc cttaaataaa attacacaga atagtggaaa
     1801 tgatgttaga aactttgaag aatttaaaaa tggagagtca ttcaacttat atgaacaaga
     1861 gttggtagaa aggtggaatt tagctgctgc ttctgacata ttaagaatat ctgcattaaa
     1921 agaaattggt ggtatgtatt tagatgttga tatgttacca ggaatacaac cagacttatt
     1981 tgagtctata gagaaaccta gttcagtaac agtggatttt tgggaaatga caaagttaga
     2041 agctataatg aaatacaaag aatatatacc agaatatacc tcagaacatt ttgacatgtt
     2101 agacgaagaa gttcaaagta gttttgaatc tgttctagct tctaagtcag ataaatcaga
     2161 aatattctca tcacttggtg atatggaggc atcaccacta gaagttaaaa ttgcatttaa
     2221 tagtaagggt attataaatc aagggctaat ttctgtgaaa gactcatatt gtagcaattt
     2281 aatagtaaaa caaatcgaga atagatataa aatattgaat aatagtttaa atccagctat
     2341 tagcgaggat aatgatttta atactacaac gaataccttt attgatagta taatggctga
     2401 agctaatgca gataatggta gatttatgat ggaactagga aagtatttaa gagttggttt
     2461 cttcccagat gttaaaacta ctattaactt aagtggccct gaagcatatg cggcagctta
     2521 tcaagattta ttaatgttta aagaaggcag tatgaatatc catttgatag aagctgattt
     2581 aagaaacttt gaaatctcta aaactaatat ttctcaatca actgaacaag aaatggctag
     2641 cttatggtca tttgacgatg caagagctaa agctcaattt gaagaatata aaaggaatta
     2701 ttttgaaggt tctcttggtg aagatgataa tcttgatttt tctcaaaata tagtagttga
     2761 caaggagtat cttttagaaa aaatatcttc attagcaaga agttcagaga gaggatatat
     2821 acactatatt gttcagttac aaggagataa aattagttat gaagcagcat gtaacttatt
     2881 tgcaaagact ccttatgata gtgtactgtt tcagaaaaat atagaagatt cagaaattgc
     2941 atattattat aatcctggag atggtgaaat acaagaaata gacaagtata aaattccaag
     3001 tataatttct gatagaccta agattaaatt aacatttatt ggtcatggta aagatgaatt
     3061 taatactgat atatttgcag gttttgatgt agattcatta tccacagaaa tagaagcagc
     3121 aatagattta gctaaagagg atatttctcc taagtcaata gaaataaatt tattaggatg
     3181 taatatgttt agctactcta tcaacgtaga ggagacttat cctggaaaat tattacttaa
     3241 agttaaagat aaaatatcag aattaatgcc atctataagt caagactcta ttatagtaag
     3301 tgcaaatcaa tatgaagtta gaataaatag tgaaggaaga agagaattat tagaccattc
     3361 tggtgaatgg ataaacaaag aagaaagtat tataaaggat atttcatcaa aagaatatat
     3421 atcatttaat cctaaagaaa ataaaattac agtaaaatct aaaaatttac ctgagctatc
     3481 tacattatta caagaaatta gaaataattc taattcaagt gatattgaac tagaagaaaa
     3541 agtaatgtta acagaatgtg agataaatgt tatttcaaat atagatacgc aaattgttga
     3601 ggaaaggatt gaagaagcta agaatttaac ttctgactct attaattata taaaagatga
     3661 atttaaacta atagaatcta tttctgatgc actatgtgac ttaaaacaac agaatgaatt
     3721 agaagattct cattttatat cttttgagga catatcagag actgatgagg gatttagtat
     3781 aagatttatt aataaagaaa ctggagaatc tatatttgta gaaactgaaa aaacaatatt
     3841 ctctgaatat gctaatcata taactgaaga gatttctaag ataaaaggta ctatatttga
     3901 tactgtaaat ggtaagttag taaaaaaagt aaatttagat actacacacg aagtaaatac
     3961 tttaaatgct gcatttttta tacaatcatt aatagaatat aatagttcta aagaatctct
     4021 tagtaattta agtgtagcaa tgaaagtcca agtttacgct caattattta gtactggttt
     4081 aaatactatt acagatgcag ccaaagttgt tgaattagta tcaactgcat tagatgaaac
     4141 tatagactta cttcctacat tatctgaagg attacctata attgcaacta ttatagatgg
     4201 tgtaagttta ggtgcagcaa tcaaagagct aagtgaaacg agtgacccat tattaagaca
     4261 agaaatagaa gctaagatag gtataatggc agtaaattta acaacagcta caactgcaat
     4321 cattacttca tctttgggga tagctagtgg atttagtata cttttagttc ctttagcagg
     4381 aatttcagca ggtataccaa gcttagtaaa caatgaactt gtacttcgag ataaggcaac
     4441 aaaggttgta gattatttta aacatgtttc attagttgaa actgaaggag tatttacttt
     4501 attagatgat aaaataatga tgccacaaga tgatttagtg atatcagaaa tagattttaa
     4561 taataattca atagttttag gtaaatgtga aatctggaga atggaaggtg gttcaggtca
     4621 tactgtaact gatgatatag atcacttctt ttcagcacca tcaataacat atagagagcc
     4681 acacttatct atatatgacg tattggaagt acaaaaagaa gaacttgatt tgtcaaaaga
     4741 tttaatggta ttacctaatg ctccaaatag agtatttgct tgggaaacag gatggacacc
     4801 aggtttaaga agcttagaaa atgatggcac aaaactgtta gaccgtataa gagataacta
     4861 tgaaggtgag ttttattgga gatattttgc ttttatagct gatgctttaa taacaacatt
     4921 aaaaccaaga tatgaagata ctaatataag aataaattta gatagtaata ctagaagttt
     4981 tatagttcca ataataacta cagaatatat aagagaaaaa ttatcatatt ctttctatgg
     5041 ttcaggagga acttatgcat tgtctctttc tcaatataat atgggtataa atatagaatt
     5101 aagtgaaagt gatgtttgga ttatagatgt tgataatgtt gtgagagatg taactataga
     5161 atctgataaa attaaaaaag gtgatttaat agaaggtatt ttatctacac taagtattga
     5221 agagaataaa attatcttaa atagccatga gattaatttt tctggtgagg taaatggaag
     5281 taatggattt gtttctttaa cattttcaat tttagaagga ataaatgcaa ttatagaagt
     5341 tgatttatta tctaaatcat ataaattact tatttctggc gaattaaaaa tattgatgtt
     5401 aaattcaaat catattcaac agaaaataga ttatatagga ttcaatagcg aattacagaa
     5461 aaatatacca tatagctttg tagatagtga aggaaaagag aatggtttta ttaatggttc
     5521 aacaaaagaa ggtttatttg tatctgaatt acctgatgta gttcttataa gtaaggttta
     5581 tatggatgat agtaagcctt catttggata ttatagtaat aatttgaaag atgtcaaagt
     5641 tataactaaa gataatgtta atatattaac aggttattat cttaaggatg atataaaaat
     5701 ctctctttct ttgactctac aagatgaaaa aactataaag ttaaatagtg tgcatttaga
     5761 tgaaagtgga gtagctgaga ttttgaagtt catgaataga aaaggtaata caaatacttc
     5821 agattcttta atgagctttt tagaaagtat gaatataaaa agtattttcg ttaatttctt
     5881 acaatctaat attaagttta tattagatgc taattttata ataagtggta ctacttctat
     5941 tggccaattt gagtttattt gtgatgaaaa tgataatata caaccatatt tcattaagtt
     6001 taatacacta gaaactaatt atactttata tgtaggaaat agacaaaata tgatagtgga
     6061 accaaattat gatttagatg attctggaga tatatcttca actgttatca atttctctca
     6121 aaagtatctt tatggaatag acagttgtgt taataaagtt gtaatttcac caaatattta
     6181 tacagatgaa ataaatataa cgcctgtata tgaaacaaat aatacttatc cagaagttat
     6241 tgtattagat gcaaattata taaatgaaaa aataaatgtt aatatcaatg atctatctat
     6301 acgatatgta tggagtaatg atggtaatga ttttattctt atgtcaacta gtgaagaaaa
     6361 taaggtgtca caagttaaaa taagattcgt taatgttttt aaagataaga ctttggcaaa
     6421 taagctatct tttaacttta gtgataaaca agatgtacct gtaagtgaaa taatcttatc
     6481 atttacacct tcatattatg aggatggatt gattggctat gatttgggtc tagtttcttt
     6541 atataatgag aaattttata ttaataactt tggaatgatg ctatctggat taatatatat
     6601 taatgattca ttatattatt ttaaaccacc agtaaataat ttgataactg gatttgtgac
     6661 tgtaggcgat gataaatact actttaatcc aattaatggt ggagctgctt caattggaga
     6721 gacaataatt gatgacaaaa attattattt caaccaaagt ggagtgttac aaacaggtgt
     6781 atttagtaca gaagatggat ttaaatattt tgccccagct aatacacttg atgaaaacct
     6841 agaaggagaa gcaattgatt ttactggaaa attaattatt gacgaaaata tttattattt
     6901 tgatgataat tatagaggag ctgtagaatg gaaagaatta gatggtgaaa tgcactattt
     6961 tagcccagaa acaggtaaag cttttaaagg tctaaatcaa ataggtgatg ataaatacta
     7021 tttcaattct gatggagtta tgcaaaaagg atttgttagt ataaatgata ataaacacta
     7081 ttttgatgat tctggtgtta tgaaagtagg ttacactgaa atagatggca agcatttcta
     7141 ctttgctgaa aacggagaaa tgcaaatagg agtatttaat acagaagatg gatttaaata
     7201 ttttgctcat cataatgaag atttaggaaa tgaagaaggt gaagaaatct catattctgg
     7261 tatattaaat ttcaataata aaatttacta ttttgatgat tcatttacag ctgtagttgg
     7321 atggaaagat ttagaggatg gttcaaagta ttattttgat gaagatacag cagaagcata
     7381 tataggtttg tcattaataa atgatggtca atattatttt aatgatgatg gaattatgca
     7441 agttggattt gtcactataa atgataaagt cttctacttc tctgactctg gaattataga
     7501 atctggagta caaaacatag atgacaatta tttctatata gatgataatg gtatagttca
     7561 aattggtgta tttgatactt cagatggata taaatatttt gcacctgcta atactgtaaa
     7621 tgataatatt tacggacaag cagttgaata tagtggttta gttagagttg gggaagatgt
     7681 atattatttt ggagaaacat atacaattga gactggatgg atatatgata tggaaaatga
     7741 aagtgataaa tattatttca atccagaaac taaaaaagca tgcaaaggta ttaatttaat
     7801 tgatgatata aaatattatt ttgatgagaa gggcataatg agaacgggtc ttatatcatt
     7861 tgaaaataat aattattact ttaatgagaa tggtgaaatg caatttggtt atataaatat
     7921 agaagataag atgttctatt ttggtgaaga tggtgtcatg cagattggag tatttaatac
     7981 accagatgga tttaaatact ttgcacatca aaatactttg gatgagaatt ttgagggaga
     8041 atcaataaac tatactggtt ggttagattt agatgaaaag agatattatt ttacagatga
     8101 atatattgca gcaactggtt cagttattat tgatggtgag gagtattatt ttgatcctga
     8161 tacagctcaa ttagtgatta gtgaatagat aaaaatatgt taaatatatc ctcttatact
     8221 taaatatata aaaataaaca aaatgataca ctacataaag tgttctatct aatatgaaga
     8281 tttaccaata aaaaggtgga ctatgatgaa tgcacagtag ttcacctttt tatatttcta
     8341 atggtaacaa aatatttttt tatataaacc taggaggcgt tatgaatatg acaatatctt
     8401 ttttatcaga gcatatattt ataaagttag taattttaac tatatcattt gatacattat
     8461 taggatgttt aagtgcaata aaaagtcgta aatttaattc tagttttgga atagatggag
     8521 gaatcagaaa agtagcaatg atagcatgta tatttttttt atcagtagtt gacattctta
     8581 caaagtttaa ctttttattt atgttaccac aagattgtat caatttttta agactaaaac
     8641 atcttggaat atctgaattt ttctctattt tatttatttt atatgaaagt gtaagtatat
     8701 taaaaaatat gtgcttatgt ggattaccag tacctaagag attaaaggaa aaaatagcaa
     8761 ttttactaga tgcaatgaca gatgaaatga atgctaagga tgaaaagtaa gtaatggtag
     8821 atataataaa gatattaaca aataaaaagt gttatccaaa taagaatagc tgaaagttat
     8881 cataattcat gaaactaata atgaaaacga gggagcagat gccaagagac acacaagtat
     8941 taaatacata taatttcgaa gcaagtgttc attactatat agatgacaag gtagtatatc
     9001 aaacattggt tcacaaagat ggtgcatggt cagttggtaa aatctattaa gctacattag
     9061 ttacagatat cacaaactat aatagttaaa catagaaata tgtgtaaatt gtgatggaaa
     9121 ttattcaaaa acacaaaaat acgtgatgaa ggacaaaatg atatagaaaa taagtatcaa
     9181 accttaataa atgatttaat tgatagttta aaagttatag gaaaaatata taaagaaata
     9241 aaaacattaa aaaaatataa gatatgttta caaattacta tcagacaatc tccttatcta
     9301 atagaagagt caattaacta attgagtatc tttaaattga aatgttagga agtgatttaa
     9361 atatgaaaac ttaaattata aaaaatcaat attaatttat ttttaaaaaa tagaaaggag
     9421 tgtataagat ttattttcaa agtttaaaaa caagaaaatc aatttaaatt tcagaaggaa
     9481 taaatgtggt tatagaagtg gatttattat caaaaataat aatactagga ggtttttatg
     9541 tctttaatat ctaaagaaga gttaataaaa ctcgcatata gcattagacc aagagaaaat
     9601 gagtataaaa ctatactaac taatttagac gaatataata agttaactac aaacaataat
     9661 gaaaataaat atttacaatt aaaaaaacta aatgaatcaa ttgatgtttt tatgaataaa
     9721 tataaaactt caagcagaaa tagagcactc tctaatctaa aaaaagatat attaaaagaa
     9781 gtaattctta ttaaaaattc caatacaagc cctgtagaaa aaaatttaca ttttgtatgg
     9841 ataggtggag aagtcagtga tattgctctt gaatacataa aacaatgggc tgatattaat
     9901 gcagaatata atattaaact gtggtatgat agtgaagcat tcttagtaaa tacactaaaa
     9961 aaggctatag ttgaatcttc taccactgaa gcattacagc tactagagga agagattcaa
    10021 aatcctcaat ttgataatat gaaattttac aaaaaaagga tggaatttat atatgataga
    10081 caaaaaaggt ttataaatta ttataaatct caaatcaata aacctacagt acctacaata
    10141 gatgatatta taaagtctca tctagtatct gaatataata gagatgaaac tgtattagaa
    10201 tcatatagaa caaattcttt gagaaaaata aatagtaatc atgggataga tatcagggct
    10261 aatagtttgt ttacagaaca agagttatta aatatttata gtcaggagtt gttaaatcgt
    10321 ggaaatttag ctgcagcatc tgacatagta agattattag ccctaaaaaa ttttggcgga
    10381 gtatatttag atgttgatat gcttccaggt attcactctg atttatttaa aacaatatct
    10441 agacctagct ctattggact agaccgttgg gaaatgataa aattagaggc tattatgaag
    10501 tataaaaaat atataaataa ttatacatca gaaaactttg ataaacttga tcaacaatta
    10561 aaagataatt ttaaactcat tatagaaagt aaaagtgaaa aatctgagat attttctaaa
    10621 ttagaaaatt taaatgtatc tgatcttgaa attaaaatag ctttcgcttt aggcagtgtt
    10681 ataaatcaag ccttgatatc aaaacaaggt tcatatctta ctaacctagt aatagaacaa
    10741 gtaaaaaata gatatcaatt tttaaaccaa caccttaacc cagccataga gtctgataat
    10801 aacttcacag atactactaa aatttttcat gattcattat ttaattcagc taccgcagaa
    10861 aactctatgt ttttaacaaa aatagcacca tacttacaag taggttttat gccagaagct
    10921 cgctccacaa taagtttaag tggtccagga gcttatgcgt cagcttacta tgatttcata
    10981 aatttacaag aaaatactat agaaaaaact ttaaaagcat cagatttaat agaatttaaa
    11041 ttcccagaaa ataatctatc tcaattgaca gaacaagaaa taaatagtct atggagcttt
    11101 gatcaagcaa gtgcaaaata tcaatttgag aaatatgtaa gagattatac tggtggatct
    11161 ctttctgaag acaatggggt agactttaat aaaaatactg ccctcgacaa aaactattta
    11221 ttaaataata aaattccatc aaacaatgta gaagaagctg gaagtaaaaa ttatgttcat
    11281 tatatcatac agttacaagg agatgatata agttatgaag caacatgcaa tttattttct
    11341 aaaaatccta aaaatagtat tattatacaa cgaaatatga atgaaagtgc aaaaagctac
    11401 tttttaagtg atgatggaga atctatttta gaattaaata aatataggat acctgaaaga
    11461 ttaaaaaata aggaaaaagt aaaagtaacc tttattggac atggtaaaga tgaattcaac
    11521 acaagcgaat ttgctagatt aagtgtagat tcactttcca atgagataag ttcattttta
    11581 gataccataa aattagatat atcacctaaa aatgtagaag taaacttact tggatgtaat
    11641 atgtttagtt atgattttaa tgttgaagaa acttatcctg ggaagttgct attaagtatt
    11701 atggacaaaa ttacttccac tttacctgat gtaaataaaa attctattac tataggagca
    11761 aatcaatatg aagtaagaat taatagtgag ggaagaaaag aacttctggc tcactcaggt
    11821 aaatggataa ataaagaaga agctattatg agcgatttat ctagtaaaga atacattttt
    11881 tttgattcta tagataataa gctaaaagca aagtccaaga atattccagg attagcatca
    11941 atatcagaag atataaaaac attattactt gatgcaagtg ttagtcctga tacaaaattt
    12001 attttaaata atcttaagct taatattgaa tcttctattg gtgattacat ttattatgaa
    12061 aaattagagc ctgttaaaaa tataattcac aattctatag atgatttaat agatgagttc
    12121 aatctacttg aaaatgtatc tgatgaatta tatgaattaa aaaaattaaa taatctagat
    12181 gagaagtatt taatatcttt tgaagatatc tcaaaaaata attcaactta ctctgtaaga
    12241 tttattaaca aaagtaatgg tgagtcagtt tatgtagaaa cagaaaaaga aattttttca
    12301 aaatatagcg aacatattac aaaagaaata agtactataa agaatagtat aattacagat
    12361 gttaatggta atttattgga taatatacag ttagatcata cttctcaagt taatacatta
    12421 aacgcagcat tctttattca atcattaata gattatagta gcaataaaga tgtactgaat
    12481 gatttaagta cctcagttaa ggttcaactt tatgctcaac tatttagtac aggtttaaat
    12541 actatatatg actctatcca attagtaaat ttaatatcaa atgcagtaaa tgatactata
    12601 aatgtactac ctacaataac agaggggata cctattgtat ctactatatt agacggaata
    12661 aacttaggtg cagcaattaa ggaattacta gacgaacatg acccattact aaaaaaagaa
    12721 ttagaagcta aggtgggtgt tttagcaata aatatgtcat tatctatagc tgcaactgta
    12781 gcttcaattg ttggaatagg tgctgaagtt actattttct tattacctat agctggtata
    12841 tctgcaggaa taccttcatt agttaataat gaattaatat tgcatgataa ggcaacttca
    12901 gtggtaaact attttaatca tttgtctgaa tctaaaaaat atggccctct taaaacagaa
    12961 gatgataaaa ttttagttcc tattgatgat ttagtaatat cagaaataga ttttaataat
    13021 aattcgataa aactaggaac atgtaatata ttagcaatgg aggggggatc aggacacaca
    13081 gtgactggta atatagatca ctttttctca tctccatcta taagttctca tattccttca
    13141 ttatcaattt attctgcaat aggtatagaa acagaaaatc tagatttttc aaaaaaaata
    13201 atgatgttac ctaatgctcc ttcaagagtg ttttggtggg aaactggagc agttccaggt
    13261 ttaagatcat tggaaaatga cggaactaga ttacttgatt caataagaga tttataccca
    13321 ggtaaatttt actggagatt ctatgctttt ttcgattatg caataactac attaaaacca
    13381 gtttatgaag acactaatat taaaattaaa ctagataaag atactagaaa cttcataatg
    13441 ccaactataa ctactaacga aattagaaac aaattatctt attcatttga tggagcagga
    13501 ggaacttact ctttattatt atcttcatat ccaatatcaa cgaatataaa tttatctaaa
    13561 gatgatttat ggatatttaa tattgataat gaagtaagag aaatatctat agaaaatggt
    13621 actattaaaa aaggaaagtt aataaaagat gttttaagta aaattgatat aaataaaaat
    13681 aaacttatta taggcaatca aacaatagat ttttcaggcg atatagataa taaagataga
    13741 tatatattct tgacttgtga gttagatgat aaaattagtt taataataga aataaatctt
    13801 gttgcaaaat cttatagttt gttattgtct ggggataaaa attatttgat atccaattta
    13861 tctaatacta ttgagaaaat caatacttta ggcctagata gtaaaaatat agcgtacaat
    13921 tacactgatg aatctaataa taaatatttt ggagctatat ctaaaacaag tcaaaaaagc
    13981 ataatacatt ataaaaaaga cagtaaaaat atattagaat tttataatga cagtacatta
    14041 gaatttaaca gtaaagattt tattgctgaa gatataaatg tatttatgaa agatgatatt
    14101 aatactataa caggaaaata ctatgttgat aataatactg ataaaagtat agatttctct
    14161 atttctttag ttagtaaaaa tcaagtaaaa gtaaatggat tatatttaaa tgaatccgta
    14221 tactcatctt accttgattt tgtgaaaaat tcagatggac accataatac ttctaatttt
    14281 atgaatttat ttttggacaa tataagtttc tggaaattgt ttgggtttga aaatataaat
    14341 tttgtaatcg ataaatactt tacccttgtt ggtaaaacta atcttggata tgtagaattt
    14401 atttgtgaca ataataaaaa tatagatata tattttggtg aatggaaaac atcgtcatct
    14461 aaaagcacta tatttagcgg aaatggtaga aatgttgtag tagagcctat atataatcct
    14521 gatacgggtg aagatatatc tacttcacta gatttttcct atgaacctct ctatggaata
    14581 gatagatata tcaataaagt attgatagca cctgatttat atacaagttt aataaatatt
    14641 aataccaatt attattcaaa tgagtactac cctgagatta tagttcttaa cccaaataca
    14701 ttccacaaaa aagtaaatat aaatttagat agttcttctt ttgagtataa atggtctaca
    14761 gaaggaagtg actttatttt agttagatac ttagaagaaa gtaataaaaa aatattacaa
    14821 aaaataagaa tcaaaggtat cttatctaat actcaatcat ttaataaaat gagtatagat
    14881 tttaaagata ttaaaaaact atcattagga tatataatga gtaattttaa atcatttaat
    14941 tctgaaaatg aattagatag agatcattta ggatttaaaa taatagataa taaaacttat
    15001 tactatgatg aagatagtaa attagttaaa ggattaatca atataaataa ttcattattc
    15061 tattttgatc ctatagaatt taacttagta actggatggc aaactatcaa tggtaaaaaa
    15121 tattattttg atataaatac tggagcagct ttaactagtt ataaaattat taatggtaaa
    15181 cacttttatt ttaataatga tggtgtgatg cagttgggag tatttaaagg acctgatgga
    15241 tttgaatatt ttgcacctgc caatactcaa aataataaca tagaaggtca ggctatagtt
    15301 tatcaaagta aattcttaac tttgaatggc aaaaaatatt attttgataa tgactcaaaa
    15361 gcagtcactg gatggagaat tattaacaat gagaaatatt actttaatcc taataatgct
    15421 attgctgcag tcggattgca agtaattgac aataataagt attatttcaa tcctgacact
    15481 gctatcatct caaaaggttg gcagactgtt aatggtagta gatactactt tgatactgat
    15541 accgctattg cctttaatgg ttataaaact attgatggta aacactttta ttttgatagt
    15601 gattgtgtag tgaaaatagg tgtgtttagt acctctaatg gatttgaata ttttgcacct
    15661 gctaatactt ataataataa catagaaggt caggctatag tttatcaaag taaattctta
    15721 actttgaatg gtaaaaaata ttactttgat aataactcaa aagcagttac cggatggcaa
    15781 actattgata gtaaaaaata ttactttaat actaacactg ctgaagcagc tactggatgg
    15841 caaacta
//
LOCUS       DAEOSP010000078        15773 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.078, whole genome shotgun sequence.
ACCESSION   DAEOSP010000078 DAEOSP010000000
VERSION     DAEOSP010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.078"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            122..679
                     /locus_tag="KRH54_001963"
     CDS             122..679
                     /locus_tag="KRH54_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8579620.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKAAIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLVDSIDTVLLGIHKKYD"
     gene            complement(1029..1391)
                     /locus_tag="KRH54_001964"
     CDS             complement(1029..1391)
                     /locus_tag="KRH54_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8579621.1"
                     /translation="MEIDYKAIGQRIKIARIKKGITQESVANIINMTPSHMSNVETGK
                     TKVSLPTLIAIANALSVSVDTLLCDNVLSSKVIFEKEAKDIFSDCDEYEIRSLVELLK
                     SAKTVIRKDREIRKQFEK"
     gene            complement(1825..2634)
                     /locus_tag="KRH54_001965"
     CDS             complement(1825..2634)
                     /locus_tag="KRH54_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8579622.1"
                     /translation="MIKKLYKTSEFAELCGVKKHTLYHYDEIELLKPSITHENGYRYY
                     TIDQFEKFNIISVLKKAGTPLEEIKQYLNDLDTNTFLNVLHRKLMDLQEEVKKIEKMC
                     SMLRNVISTMEIHLNVNTEKIEIVNCEEEYLIATKIPEAVLTDEKTMLQGIGNHLQYC
                     LLHDYNFHVGEIVLEKNIKQGIFRESYYYSLIKNKVDDERLFIKPKGNYAVMYHQGSY
                     DSLYKTYQNMKNQLEEQGYHIVGNIYEEDLIDYLSEHNAENYIQKISMQVI"
     gene            2726..3349
                     /locus_tag="KRH54_001966"
     CDS             2726..3349
                     /locus_tag="KRH54_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HBG8579623.1"
                     /translation="MKIITISREFGSGGRELGKQLADILNFDYYDKEIITAIARKKKI
                     DENYVSYILENHGWRNVPLTMCTSFTNILSMQSMKTDLLIEQSNVINQIASTGKDCVI
                     VGRNADILLKKYQPFNVFVCANMNFKIQRCIEKKLNNEHISKKELERKIREIDKNRAR
                     SREFFTSSLWGNQTSYHIIVNTSEWNIKHLSYAIANFSEYWFRRYEQ"
     gene            3346..4671
                     /locus_tag="KRH54_001967"
     CDS             3346..4671
                     /locus_tag="KRH54_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8579624.1"
                     /translation="MIQLSDNFNYKRLLKFTFPTIIMLVISSIYGVVDGYFVSNFVGK
                     TAFTAVNFIMPFLLILGCVGFLFGTGGGALIAKTMGEGKKEEANEQFSLLIVTSAGCG
                     IILAVLGIVTMPWIASAMGADGQLLTDSILYGCVVIIAIPAYILQCEFQCLFATAEKP
                     KLGLYVAIMAGITNIVLDALFITVFKWGLVGAALATAIGQYVGGIIPLLYFSRQNNSL
                     LKFKKPKFDCDVLKKTVANGSSELMTNVSTSVVSMLYNAQLLKYAGEDGVASYGVLLY
                     VALLFQAVFIGYSVGTAPIISYHYGAKNYDELKNLRKRSLIVIICFAVFMFIIGEFLS
                     KPIAFIFVGYDNNLMEMTLRAFMIYSFSFLFSGFGVFGSSFFTALNDGLTSALIAFMR
                     ILVFQVVSVLIFPIFWGVNGIWFSIVGAEILATIMTAFFIIRKQKKYHY"
     gene            4692..5231
                     /locus_tag="KRH54_001968"
     CDS             4692..5231
                     /locus_tag="KRH54_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8579625.1"
                     /translation="MSLTIQKVTSYFKDLEKFEELNNEAFPDEERMSIQEMLNLVQSK
                     KIEVSALYDKQQFIGFYVLMIENHIGYILFLAISPEQRSKGYGGKALSLMKKQYPNCQ
                     IVLDMETIEDSAPNLQQRILRKQFYLQNGFYETEYLMEYKGLEMEVLCNQKTFQKNEF
                     ETLLKNLKIREIPFHLWKK"
     gene            5250..6641
                     /locus_tag="KRH54_001969"
     CDS             5250..6641
                     /locus_tag="KRH54_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HBG8579626.1"
                     /translation="MKRRLAGEWEPIKGVMLAWPPFLPHKYIVDLTQNYSVYMCVPNK
                     EEIEIAKYYLSKWGGNLKNLIFCIAPQGFDTPWVRDWGMHPIFDENGKLFLAGAEYKM
                     STPFVSINKPEIMFDCEHNLLTTNSYEQKEDQSQELIAKQIGLPFMKLPYALTGGNLM
                     SDGFNKLMSMHILQTENRAKGISDEEFFSMVARDTGMNEYYIFSNFANYGLQHIDCYL
                     KIIDEETLLVSRTPKEHYLYERYEKILEEVKKVESRYGRPFKIFRIDIVPFQKDSDEL
                     TAYVNSVILNSRVYVPMYGIEQDKIAIKQWQNALPGYEIKGYEFDFEKEPDIIKNRTG
                     YVRTGWGNEDVIHCRTRAIWDENMLYISVKRLEEIVSENDALEVTIQIVDYSKAGLDY
                     ENCRLFYRYYGFQTWESIRLEETTEAEIFFANMIGKSGDMIEYFVQAKSCSGMCETMP
                     PVAPKGTYKVTIK"
     gene            6656..7747
                     /gene="ytvI"
                     /locus_tag="KRH54_001970"
     CDS             6656..7747
                     /gene="ytvI"
                     /locus_tag="KRH54_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG8579627.1"
                     /translation="MNEQDHTSYQNKQRFLINFCYLVVILGLLMLLGKYAFSVLLPFV
                     FAFLIASLLNKPVLYISEKLLIKRGIVATISVFLFFLIAGIIVSVIGTYLIYGIKEIF
                     HFLPTMFEELVIPLTETVIKEVELFSGSFNFSFIELIEANMPVLMNEMSVFIVSLSNN
                     IVSGITDIVSSVPLLFMKTIITIVASIFIAIDYPTIKRFIVLQIPKNKSYLLAEAKAF
                     TIDTIIKCGFSYLLIFAITFSELYVGFVIINISYAGIIALFIALLDILPVLGTGSILI
                     PWCIISLFLGDYSMALGIALLYIVITIIRNIIEPKLVGKHMELHPVLALASMLTGLHF
                     FGFIGLFGIPLLIAFLKKLNDKEIIHILH"
     misc_binding    8050..8263
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8517..9551
                     /locus_tag="KRH54_001971"
     CDS             8517..9551
                     /locus_tag="KRH54_001971"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBG8579628.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGSAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            9592..10809
                     /gene="argJ"
                     /locus_tag="KRH54_001972"
     CDS             9592..10809
                     /gene="argJ"
                     /locus_tag="KRH54_001972"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBG8579629.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            10824..11684
                     /gene="argB"
                     /locus_tag="KRH54_001973"
     CDS             10824..11684
                     /gene="argB"
                     /locus_tag="KRH54_001973"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBG8579630.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            11714..12901
                     /locus_tag="KRH54_001974"
     CDS             11714..12901
                     /locus_tag="KRH54_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBG8579631.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLCSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            13005..14027
                     /gene="argF"
                     /locus_tag="KRH54_001975"
     CDS             13005..14027
                     /gene="argF"
                     /locus_tag="KRH54_001975"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBG8579632.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLV"
     gene            14154..14771
                     /locus_tag="KRH54_001976"
     CDS             14154..14771
                     /locus_tag="KRH54_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBG8579633.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIESFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(14991..15689)
                     /locus_tag="KRH54_001977"
     CDS             complement(14991..15689)
                     /locus_tag="KRH54_001977"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBG8579634.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
BASE COUNT         5883 a         1750 c         2787 g         5353 t
ORIGIN      
        1 taattaaact gtcaagattt tataatagta taaagaattt tataaattac aataattgac
       61 gaaactaatt agattagtta aactaataaa gtaattaatt ttaaatgaaa gagggaaaat
      121 catgcgtgtt agtagagaaa tagttataca agctacatca gatatagtta ataaagatgg
      181 gcttaacaaa gtttctttaa aagttattgc agaaaagcta ggaatacgta caccatcttt
      241 gtacaatcat atagaaagtc tagatgattt attacttgaa gtagctcata aaggaatgag
      301 agagatgaat agtcaaatga taaaggcggc tattggaaat tttggagata cagctataaa
      361 gtgtataagt atagcatact ttaattttat aatttctaat cctggtgttt atgaaacaat
      421 tcagtgggct acttggcatg gaaataatga aacattagaa atctttgatg aatataaatc
      481 cttattagaa aaacttattc tttcttgtaa tctaaataca aagaatacag gtgaagtatt
      541 aaacttaata atgagtgtac ttcatggtta ttcgactttg caacttggga aatcactttt
      601 gagtaaagaa gaagctataa aaggtctagt cgattctatt gatactgtgt tattaggtat
      661 tcataaaaaa tatgattaag gaggtaaata aattatttta acttatttat tgaacacttc
      721 attacgtggg atgtaaaata aaattttatc atttttatga aaggaaaata gtatatttca
      781 agaatattta ctttctgaat tggtaaaccc tatttttact gttgtcgttg ataatgaaaa
      841 ggtagctgta aaattccttg ataatcagac aaaagcgaca caggtatcat agtataaatt
      901 gatatttcat aaggacagta attggaaaat tattggatag gttatttgat tatataaaaa
      961 tgattatata aaaaaagaaa agaccttagc taacaacaca tatcaattta taccttgaag
     1021 ctgtggtttt atttttcaaa ttgtttgcgg atttctctgt ctttacggat tacagttttt
     1081 gctgatttta gcagttcaac taaagaacgg atttcatatt catcacaatc tgagaaaata
     1141 tctttggctt ccttttcaaa aattactttg gaagaaagta cattatcaca taagagagta
     1201 tcgactgata cagaaagtgc attggcaatc gcaatcaagg ttggcaggct gactttcgtt
     1261 tttcctgttt ccacgttgct catatgggat ggtgtcatgt tgataatgtt tgcaacggat
     1321 tcttgtgtta tacctttttt aattcttgca attttaattc gctgtccgat tgctttataa
     1381 tcaatttcca tagtttttgt tctcctttca ttcttttaaa tattgtaact atttacagag
     1441 cataatataa tactctatat aaatgaataa tttatataat atatatttaa gaaaaaaatt
     1501 tgcactactt ttaagacaac tacaaaagaa atggaggcat aaagcctcca tgttaagatt
     1561 ttataaaaag atagtaaaaa actgattaaa tggttaacga aaataaaaag ctatttctgt
     1621 atctgaaatt catgaggaaa tgcagtttgc attgaggaaa caaataatag caatgaacct
     1681 gaatttctac aaaaatttgg aaataacata ccaactttag aagataaaaa aggacatttt
     1741 aagcaaatta ttatagacaa tacgagatat taaaaaaggc gtgtatttct catgtgattt
     1801 gagatataca cgctttttat attttcaaat aacctgcata gaaatttttt gaatgtagtt
     1861 ttcggcgtta tgttctgata aataatcaat taaatcttct tcataaatat ttccaacaat
     1921 atgataccct tgttcttcta actgattttt catattttga taagttttat ataaactgtc
     1981 ataactcccc tggtgataca taactgcata atttcccttt ggtttaataa aaaggcgttc
     2041 atcatctact ttatttttaa taaggctata ataatagctc tctctaaaaa ttccctgttt
     2101 tatatttttt tcaagaacaa tttctcctac atgaaaatta taatcatgta ataaacaata
     2161 ttgtaagtga tttccaatac cttgtaacat tgttttttca tctgttagta cagcttcagg
     2221 aattttagtt gcaatcagat attcttcctc acaatttaca atttcgattt tttctgtgtt
     2281 tacattgaga tgtatttcca ttgttgaaat gacatttctc aacatagaac acattttttc
     2341 aatcttcttc acttcttctt gaagatccat tagctttctg tgaagtacat taagaaaggt
     2401 attggtatct aaatcattga gatattgttt tatttcttct aaaggtgtac ctgctttttt
     2461 tagcacagaa ataatattaa atttttcaaa ttggtcaatt gtataatacc gatagccatt
     2521 ttcatgggta atactcggtt ttaataattc aatttcatca taatggtaaa gtgtatgttt
     2581 ttttacccca cacaattctg caaattcact tgtcttatat aattttttaa tcatttttaa
     2641 atctcctatt gactttaggg ttaccagata ctttaggata acatagaaag cttttatatt
     2701 caatgaaatg tgaggaaaag atattatgaa aattattaca attagccgag agtttggaag
     2761 tggtggacga gaacttggaa aacaacttgc tgatatttta aattttgact actacgataa
     2821 agaaattatc actgctatag ctagaaagaa aaaaattgac gaaaactatg tttcctacat
     2881 attagaaaat catggttgga gaaacgttcc tttaacaatg tgtacttctt ttactaatat
     2941 tttgtctatg caatccatga agacagattt gttaatagaa caatcaaatg ttatcaatca
     3001 gattgccagt acaggtaagg attgtgttat tgttggtaga aatgcagata tattattaaa
     3061 gaagtaccag ccatttaacg tttttgtttg tgcaaatatg aactttaaaa tacaacgctg
     3121 tattgaaaaa aagttaaaca atgaacatat ttcaaaaaaa gaattagaaa gaaagataag
     3181 agagattgat aaaaatcgag caagatcaag agaatttttt acgagctcct tatggggaaa
     3241 tcaaacttcc tatcatatta tagttaatac atctgaatgg aatataaaac atctttctta
     3301 tgcaattgct aacttttcag agtattggtt taggaggtat gaacaatgat acagttgtca
     3361 gataatttta attataaacg cttattaaaa tttacatttc ctacaattat tatgctggta
     3421 ataagttcta tttatggggt tgttgatgga tattttgttt caaattttgt agggaaaact
     3481 gcttttactg ctgtcaattt tattatgcca tttttgctaa tcttagggtg tgttggcttt
     3541 ctttttggaa caggtggtgg agctttaatt gctaaaacaa tgggtgaagg aaaaaaagaa
     3601 gaagcaaacg aacagttttc gttactcatt gttacttctg ctggttgtgg tattattcta
     3661 gctgttttag gtatagtaac tatgccatgg attgcttctg ctatgggagc agatggtcaa
     3721 ttattgacag atagtattct ttatggatgt gtggtaatta tcgctattcc tgcctatatt
     3781 ttgcagtgcg aatttcagtg tttatttgca acagcagaaa agccaaaact tggattatat
     3841 gttgcaatta tggctggaat tacaaatatt gttttagacg cattatttat cactgttttt
     3901 aaatggggat tggtaggtgc agcactggca actgcaatcg gtcaatatgt tggtggtatc
     3961 attccattac tttattttag ccgacaaaac aacagtttgc ttaaatttaa gaaacctaaa
     4021 tttgattgtg atgttttgaa aaagactgtt gcaaatggtt cttcagaact aatgactaat
     4081 gtttccacat ctgttgtgag tatgctgtat aatgctcaat tactaaaata tgctggcgaa
     4141 gatggcgttg catcctatgg tgttcttctt tatgtagcgt tactatttca agctgttttt
     4201 attgggtatt ctgttggtac tgcccctata atcagttatc attatggagc aaaaaattat
     4261 gacgaattaa aaaatttaag aaaaagaagt ttgattgtaa ttatttgttt tgctgtattt
     4321 atgtttataa taggcgaatt tttatcaaaa ccgattgctt ttatttttgt gggttacgat
     4381 aataatttaa tggaaatgac attaagagca tttatgattt actctttttc ctttttattt
     4441 tctggttttg gtgtttttgg ttctagtttc tttactgcat taaatgacgg tttaacttct
     4501 gcgcttattg cttttatgcg tattttagta tttcaagttg tatcagtact tatattccct
     4561 atattctggg gagtaaatgg gatatggttt tctattgttg gtgctgaaat attggcaact
     4621 attatgacgg ctttctttat aataagaaaa cagaaaaaat atcattatta aatcaatttg
     4681 gaggtatcta tatgagctta acaatacaaa aagtaacttc atattttaaa gatttagaaa
     4741 aatttgaaga acttaataac gaggcatttc cagatgaaga acgtatgtct atacaagaaa
     4801 tgcttaattt agttcaaagt aaaaaaatag aagtatctgc tttatatgat aagcaacaat
     4861 ttattggttt ttatgttttg atgattgaaa atcatatagg atatatatta tttcttgcaa
     4921 tttccccaga acaacgctct aaaggatatg gtggaaaagc actgtcactt atgaaaaaac
     4981 aatatcccaa ttgtcaaatt gttcttgata tggaaactat agaggacagt gctcctaatt
     5041 tacagcaaag aattttaagg aagcaatttt atcttcagaa tgggttttat gaaacagaat
     5101 atctcatgga gtataaggga ctagaaatgg aagttttatg taatcagaaa acctttcaaa
     5161 aaaatgaatt tgaaacattg ttgaaaaatc ttaaaattag agaaattcca tttcatttgt
     5221 ggaaaaagta agaaagtgag gtattagaaa tgaaaagacg tttagctggg gaatgggaac
     5281 cgattaaagg tgtcatgctt gcatggccac cttttcttcc ccataagtat attgtagatt
     5341 taacgcaaaa ttattctgta tacatgtgtg taccaaataa agaggaaatt gaaattgcaa
     5401 aatattattt aagtaagtgg ggtggaaatt taaaaaattt gattttctgc attgctccac
     5461 agggatttga tacaccatgg gttcgtgatt ggggtatgca ccctattttt gatgaaaacg
     5521 gaaaactctt tttggctggt gcagaatata agatgtcaac accatttgta tccataaata
     5581 aaccagaaat catgtttgat tgcgaacata acctattaac tactaactct tatgaacaaa
     5641 aagaagatca atcacaagag ttgattgcga aacagattgg tttgccattt atgaaattac
     5701 cttatgcact aacaggcgga aatcttatgt cagatggttt caataaattg atgtctatgc
     5761 acattttaca gacagaaaat cgtgcgaaag gaatttctga cgaagaattt ttttctatgg
     5821 tggcaagaga tactggaatg aatgagtatt atatattttc taattttgct aattatggtt
     5881 tacaacatat tgactgttat ttaaaaataa ttgatgaaga aacattactt gtgagcagaa
     5941 cgccaaaaga acactatttg tatgaaagat atgaaaaaat tttagaagaa gtaaaaaagg
     6001 tagaaagcag atatggacgt ccattcaaaa tctttcgtat agatattgtt ccgtttcaaa
     6061 aggacagtga tgaacttact gcttatgtta attctgtgat tttaaatagt cgtgtctatg
     6121 ttccaatgta tggaattgaa caggataaaa ttgcaataaa acaatggcag aacgctttgc
     6181 ctggatatga aattaaagga tatgagtttg attttgaaaa agagccagat attataaaaa
     6241 atcgtacagg atatgtgaga actggctggg gaaatgaaga tgttattcat tgcaggacaa
     6301 gagcaatttg ggatgagaat atgctctata tttccgtgaa aagattagaa gaaatcgttt
     6361 cagagaatga tgctttagaa gttactattc aaatagttga ttatagtaaa gctggcttag
     6421 attatgaaaa ttgtagactg ttttatcgat actatggttt tcaaacatgg gaaagtatta
     6481 ggttagaaga aactacagaa gcagaaatat tttttgccaa tatgatagga aagtcaggtg
     6541 atatgattga atattttgtg caagcaaaaa gttgttctgg aatgtgtgaa acaatgcccc
     6601 ctgttgctcc aaaaggcaca tataaagtta ctataaagta ggaggtgcat tttatttgaa
     6661 tgaacaagat catacatcat accaaaataa gcagagattt ttaataaatt tctgttattt
     6721 ggtagtgata ttaggcttac taatgttatt agggaaatat gcgttttctg tattacttcc
     6781 ttttgttttt gcatttttaa tagcaagttt attgaataaa ccagtcttat acataagtga
     6841 aaaattacta ataaagcgag gtattgtagc aaccatatct gtttttttat tctttttaat
     6901 tgcaggaata atagtaagtg taataggtac atatctaata tatggaataa aggaaatttt
     6961 tcatttccta ccaaccatgt ttgaggaatt ggtaatacct ttgactgaaa cagttataaa
     7021 agaagtcgaa ctattttctg gttcattcaa tttttcgttt attgaattaa tagaagcaaa
     7081 tatgcctgta ttgatgaatg aaatgagtgt tttcatagtt tctctttcaa ataatatcgt
     7141 atctggtatt actgatattg tttcgtctgt tccattacta tttatgaaaa caattattac
     7201 aatcgttgct tctattttta tagcaattga ttatccaacg ataaagagat ttatagtgct
     7261 acagatacct aaaaataaaa gttatctttt agcagaagca aaagcgttta ctattgatac
     7321 cattattaaa tgtggttttt cttatctttt aatttttgct atcacttttt ctgaattata
     7381 tgtcggtttt gttattatta atatttctta tgctggaata attgctttat tcattgctct
     7441 tttagatata ctacctgttt taggaactgg aagtattcta ataccttggt gtattatcag
     7501 tttattttta ggggattatt caatggcatt aggaattgca ctactatata ttgtgattac
     7561 tattatcagg aatattattg aaccaaaatt agttggtaaa catatggagt tacatcctgt
     7621 attggcctta gccagtatgc taacaggatt acatttcttt ggatttattg ggttgttcgg
     7681 tattccattg ttaattgcgt ttttgaaaaa gttaaatgac aaagaaatca tacatatatt
     7741 acattaggaa agaatagttg tatgtatgtc tcttttatgg agaaaattgt gttttttcta
     7801 aaaaacgctg gatgaaaaat aattttgaca aaggtatagc ttttgaatga cttgattcaa
     7861 gagggagaac ttttttaaaa tatattacag caggaaattc atggtgtcct attactgtag
     7921 atatttataa ctcaatttag atacaaaatg ctggaaatta attatataga aaatatttcc
     7981 aaaaacttaa aaattatatt gacaaagtta aaaaaataaa ctatcatata aaacaataag
     8041 aaaataaatt gaaatcctgt gaaaaagaga gtagcataaa gctagttgat agagagctgg
     8101 gaatggtgga agcctagtaa cgaattttat tgtgaataga gctttggagg agattaccag
     8161 aagtaatagt atggtaattg gtgaatctcg ccttaagaga ttagagtggg taagattatc
     8221 ttatcaatta gagtggtaac gcgggtgtat attcgtctct tggtctttga ccaagaggct
     8281 tttttaattt taaatgtaca ttttgtagat actttttaat ttgagtttta atgattattg
     8341 taattttacc aataagagtg gtaccgcgga aaatcgcctc ttagtattta taatgctaag
     8401 gggttttttg ttttaattag agtattttaa tctacgtatt atcaaatgat tatcatatag
     8461 taaagtggaa agataggatt ttaagactat ttgaatataa aaatggaggg aacaaaatga
     8521 ataaaataaa agttggtatt attggtgcaa ctggatatgt cggggcagag ttgattagat
     8581 tattaatgaa tcatgataaa gtcgaagtga cagcgatagg ttcaaattcg tatgttggaa
     8641 aagatatagt tgatatatat ccaagtatag gatataaaaa taacatgata tgtatagaaa
     8701 atgaaaaagt tatagatatg tgtgatgttg tgtttacagc ccttccacat ggagttagtg
     8761 aaaagtttgt aataaaggca attaaatcga aaaagaaagt tatagattta ggtgctgact
     8821 ttagaataaa agatgaagaa gtatatagta aatggtatgg agtatctttt atagataaaa
     8881 tcttacataa aaaagctgta tatggattaa gtgaaatata caaagaagat ataaaagatg
     8941 cagatataat tgcaaatcct ggatgttatc caactagtat ttctttacca ttgatgccat
     9001 tactaagttc taaattgata aaaaataata atataataat tgattctaaa tcaggtctta
     9061 caggtgctgg tagagaatta tcgattagta gtcactttac ggaggttaat gaaaatatca
     9121 ctgcatataa aatagggaaa cacagacata ctccagaaat agaacaaaat ttaagtgaat
     9181 cctgcaaaga aaaagtaagt gtagttttta caccaaactt aatcccagta aataggggaa
     9241 tcttatcaac tatatattgt acaaaagaag ataatatttc tataaatgat atacatagga
     9301 aattaactga ttattatgaa tttaaagagt ttatagaaat tcttccatta gataaggtag
     9361 cttctttaaa gaatgttaga ttctcaaata aatgtgtaat atcattacat gaaaatggag
     9421 atacattgat aatctgttca gctatagata atatgattaa gggatcagca ggtcaagcta
     9481 tacaaaacat gaatataata tttggtattg aagaaaatac aggtttaaaa aatattgcac
     9541 catcatttta gaaataaaaa atactaaact catataatta ggaggataaa tatggagatt
     9601 ataaagggtg gagttacagt ttcagaagga ttttttgctt cagggattca ctgtggcttg
     9661 agaaaaaata aagaaaaaag agatttagct ttggtttatt cagatgtttt atgtgatgca
     9721 gcagcagtag taactcaaaa taaagtaaag ggcaatcctg tttatgttac acaggaacac
     9781 attaaaaatg gaaaggcaca agctatcata gtaaatagtg ctaatgctaa tacctttaat
     9841 ggaaaagaag gacttataaa tgcttataaa atggctaaat tcaccagtga taaattaaat
     9901 attaaagaaa gtgatgtttt ggttgcatct acaggtgtta taggaaagcc attaaatata
     9961 gatttaatag aagaaaatat ggatgaatta gtgaataatc taagtaaaca aggtcatatt
    10021 ggagcaagag aagctattat gacaactgat attataaaaa aagaaatagc agtagcaata
    10081 atgtatggag ataaaaaaat aactattgga ggtatggcaa aaggttctgg catgatacat
    10141 cctaatatgg caacaacgtt aggatttata actacagatg caaatattga tggagttttg
    10201 ctaaaagaag ctttaaaaat tgctgttgac aaaagtttta atagagttag tgtagatgga
    10261 gatacaagta ctaatgatat ggtaatgatt ctagcaaatg gaaaagcaaa aaatgataga
    10321 ataaacaaaa aagatgaaca ttatcaagta tttttgagtg cacttacata tgtctgtata
    10381 gaacttgcta aacttgttgc aaaagatgga gaaggatgta caaaactaat tgaatgctat
    10441 ataaatggag cattaagtga agaacatgca gtaaaattag caaaaacagt tatatcttct
    10501 tcattagtaa agacagctgt atttggagct gacgcaaatt ggggtagaat actgtgtgct
    10561 ttaggttatg caggtgaaga aatagatatg gaaaaagttg atattatttt tgagagtatg
    10621 aaaggatata ttgaagtatg taaaaatgga aatggattag attttaatga ggaaaaagca
    10681 aaaaaaatcc ttgaagatga cgaaataagt atattagtag atttaaatat gggcaatgcc
    10741 agaggaaatg catggggatg tgatttaagc tatgattatg taagaataaa tggaagttat
    10801 agaaattagg aggatatact ttaatgatta atatagaaaa agcaaataca cttatagaag
    10861 ctttaccata tatagaaaaa catcagggaa agactattgt agttaagtat ggtggaagtg
    10921 caatgaaaaa agatggtcta aaagaatctg ttatggaaga ccttgtgtta atgagttatg
    10981 ttggaatcaa tatagtttta gtacatggtg gaggggcaga aatcaataag atgctagcaa
    11041 aagtggatat agaaagtaaa tttgtaaatg gtcttagata tacagatgaa gagaccatgg
    11101 aaattgtaaa aatggtactt gctggaaagg ttaataaaga cctagtaaat aaaatacata
    11161 caaaaggtgg aaaagcagta gggctttgtg gaatagataa taatatgatt ttatgtgacc
    11221 catataaaaa ttatgaactt ggatttgtag gagaaatcaa aaaagtaaat gttgagctta
    11281 tagaatcttg tctaaagtca ggttatattt cagtaattgc aactataggt gttggagatg
    11341 atggagagac ttataatata aatggtgaca cagcagcttc tgctatagct aaagagttaa
    11401 atgcagataa attgatactt cttacagatg tccctggttt gttaagagag ccagatgaag
    11461 aaaaatcatt aataacagaa gttatattag aggatgttga taaattattt gaagaaggta
    11521 taattactgg aggtatgata cctaaaatag agggatgcgt agatgcctta aataacggag
    11581 taaatagggt acatattttg gatggaagag ttccacattc tataataaca gagctattta
    11641 ctgatagtgg aataggtaca ttaataagaa aagaaaatga ataaaattat aagtttaaga
    11701 ggggagaata agaatgaata acgacaatac aatttcaaaa tggaatgaat attttataga
    11761 tacatataac caacccaatt ttgtcataga ttatggtgaa ggaagttgtt tttttgatac
    11821 taatggcaat aaatacatag attttacaag tggatatgga gtatcttctc ttgggtatag
    11881 taatagtaat ttaaagaatg ctctaaaaga gcaagtagat aaattattac atacttcaaa
    11941 tttatatttt aatgagccag tgttgtgttc aggagagaaa ataattaatt caagtggaat
    12001 ggctaaagta tatttttgta attctggaac agaagcaaat gaaacagcat tcaaaatagc
    12061 aagaaagtat agtagtgaca aatatggtaa tggtagagga actatcataa gtttaaaaga
    12121 ctcttttcat ggtagaacaa tgatgtcttt aatggcaact ggtatggaca aataccataa
    12181 gtatttttat cctcttcccg aaggttttaa atatatagaa agaaataata ttgaagattt
    12241 aaaaaataat cttgattcca cagtttgtgc aataattctt gaagcgattc aaggcgaagg
    12301 tggagttaat gttcttgaaa aagattatgt tttggaaata gtaaaaatat gtcaagaaaa
    12361 ggatattgta gtgatttttg atgaagtcca atgcggtata ggaagaactg gtaaactttt
    12421 tggctatgaa tattttgatg taaaaccaga tatagttacc gtagcaaaag ggttgggagc
    12481 aggaatacca gtaggaggag tattggtaaa taaaaaactt tcaaaagtac taggaaaagg
    12541 agaccaaggt acaacttttg gaggtaattt attagctatg gtggcagctt cagttgtatt
    12601 agatgagata tctaaagatg gtttttataa tgaagtatta gaaaaaggaa attatataag
    12661 aaaatctatt gaaagtttta ataacaaagt tgtattaaag acaaaaggta ttggtctaat
    12721 gataggaatt gaaactaata ttgaatctag tattatagaa gagaaagcta gaaaaaaagg
    12781 attgcttatt ttgactgctg gaaaaaatgt attgagattt ttaccaccat taacaatcag
    12841 ttataaggaa atagatgagg ctcttgaaat cctaaaagat atattactag aaattaacta
    12901 aatattcaaa aaatagttaa ttaaaaaatt agaagtaaat aaaaaagtaa agtgctaaca
    12961 agtttaattg aatttaaaaa gtttataaga aggggcgaat taatatgaat aatataagtt
    13021 taaaaggtaa gagttttttg acattaaagg attttacaaa agaagagatt agatatttat
    13081 taactttatc aagttattta aaaactaaga aaaaagttgg tataaaaggc gatttgctac
    13141 aaggaaaaaa tatagcttta ttatttgaga aaacatcaac aagaactaga tgttcatttg
    13201 aagtagcagc tcatgatgaa ggagcacatg taacttatct aggtccaaat gattgtcata
    13261 tgggtaaaaa agagtctgta tcagatacag caaaagttct aggaagattc tatgatggaa
    13321 tagaatttag aggctttaaa caagaaacag ttgaaagtct agcaaaatat tctggtgtac
    13381 ctgtttggaa tggacttaca gatgaatatc atccaacaca aatactggca gattttttaa
    13441 cagtaattga aaatgtagat aaagatttga ataaagttaa atttgtatat gttggtgatg
    13501 ctagaaataa tatgggaaac tcactgatga taggatgtgc taaaatggga atacatttcg
    13561 tagcactagc acctaaggaa ctttggccaa atgaagaatt ggttaaggaa atgaaggaaa
    13621 tagccaagaa atccaaagga gatataatgt tgactgaaaa tattgatgat gttaaaggtg
    13681 cagatgttat atatacagat gtatgggttt ctatgggaga agaggagcaa tatgaagcaa
    13741 gaataaatca acttaaagat tatcaagtaa atatggatat gataaagaaa actgaaaatg
    13801 aaaatgttat atttttacac tgtcttccag catttcatga tttagaaact aaggttgctc
    13861 aggagattta tgaaaagttc ggattatcag aattagaggt tacaaacgag gtatttaatt
    13921 caaaatattc aaaggtattt gaagaagctg aaaatagaat gcatacaata aaagctgtta
    13981 tggtttctac tatgaccgat gtaaatatga acaataattt agtatagata tgttttaata
    14041 tagactttac tttaatgaaa atttataaaa tattttaaat atattattca aaaactctca
    14101 ttatatgtta aaataactta tgtatcaaca aattaagagg gaggatttga gatatgaaaa
    14161 aaatcatatt agctttaaca acttttttat tagcaatatc tttaacagca tgttcaagtg
    14221 caaaaccaga agatacaatt gagagttttt ttaacagtgc gaaaaagttt gactttgaag
    14281 gcatgaataa ggtaatggaa aataatgacg aaaaatataa ggatatatta aaggaattag
    14341 atacaaaaga cccaaatgcc caatatgttt tagattatct aaaacagaat gcatctaaaa
    14401 tcacatatac aataaaagac agcgaggtta aaggtgataa ggctacaatc aaagtagaat
    14461 gtaaatttat agattcaaca ccattacttc aagaaatagt agctgaggca tttacaaaga
    14521 tgattggtat gagtttttca ggacaagact taactgatga gaaaacaact gaaatgttag
    14581 tatcaattat gaaagaaaaa caaaaaagcg ttaaagagac ttatgttaca aaaaatgtag
    14641 agtttgaatg ttctaaaaag gataataaat ggataataag ttcagtaaat gatgcagtag
    14701 ctgatgtatt gttatctaat cttgttacag caggacaaga attttctaac tctatgaata
    14761 cacttgaata atattgttta ggtgtagtaa agtaataaat atatgattat ttttataaat
    14821 aaacatataa atatttatga aggataccat ttttggtatc ctttttatta atataatgaa
    14881 atacatggtt aatttctata ttgtcaagaa actatgatat atgttataag cctatactat
    14941 ttaagataaa cttataacat atatttttta taatatagtg catgaaaaat ttatttaact
    15001 aattttagat atttattgac aactgattca gccaagcatt ttgatgaatc aacaatattt
    15061 attgaaggat atgatttttc taaaactaca tttaaatcag tacaagcaat tactatattt
    15121 tctatagatt catttttaac atcttcgata agtttattcc aaatatcaat attgtgtgga
    15181 ttttctttat ccatttttat attttgaata agattgttta attttatttg ccattcatct
    15241 ttaaatataa attcatgtcc attatgtatt ataccattct ggtaaattgt agactcaaaa
    15301 gttgaactag tcgaaaaaag agttactttt tgtgagatag taggtaattt tttaactgtt
    15361 tcttctacaa tatttaatat tggtatatta atagactctt ttaattcttt aaagtatata
    15421 tgtgctgaat tgcatggcat tgcaataaaa tttacaccag ttgattcaag cttttgtaat
    15481 ccatcaatta tagtttcttt cataagttca tgatttattg gacggtcaat ataaaatggg
    15541 gtaggtaatg aatatatcat catctttgga aattcatcat catattttgc tccatattga
    15601 gattgacact catcaataat taaatcaaca aaaggggctg ttgaccttgg acccattcca
    15661 gccaaaatac caattaattt ttcattcata aaaattatct cctttatata taaatatatt
    15721 tatgtataaa ataaaaatct ataaaagcat aaaattatat tttataatag att
//
LOCUS       DAEOSP010000079        15765 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.079, whole genome shotgun sequence.
ACCESSION   DAEOSP010000079 DAEOSP010000000
VERSION     DAEOSP010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15765
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.079"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            198..1388
                     /locus_tag="KRH54_001978"
     CDS             198..1388
                     /locus_tag="KRH54_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG8579635.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            1558..2520
                     /locus_tag="KRH54_001979"
     CDS             1558..2520
                     /locus_tag="KRH54_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBG8579636.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(2642..3304)
                     /gene="zmp1"
                     /locus_tag="KRH54_001980"
     CDS             complement(2642..3304)
                     /gene="zmp1"
                     /locus_tag="KRH54_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBG8579637.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDSINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(3404..3489)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3674..6592)
                     /locus_tag="KRH54_001981"
     CDS             complement(3674..6592)
                     /locus_tag="KRH54_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG8579638.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIQEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSVLEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDANFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSVKNPGTYYLVEIMAPRGY
                     EKLKDKVKVEIGNEDIGKIVDIGNIENLKQENPPVNPPIPPDTDEPIVNPPVPPSTDK
                     PRKPSSSSDTENTIVINPPVPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      complement(6710..6795)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(6922..8100)
                     /locus_tag="KRH54_001982"
     CDS             complement(6922..8100)
                     /locus_tag="KRH54_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG8579639.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     gene            8475..8624
                     /locus_tag="KRH54_001983"
     CDS             8475..8624
                     /locus_tag="KRH54_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBG8579640.1"
                     /translation="MFFISYIYTKSPIEFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCV"
     gene            complement(8725..11499)
                     /locus_tag="KRH54_001984"
     CDS             complement(8725..11499)
                     /locus_tag="KRH54_001984"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG8579641.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAVTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(11671..12927)
                     /locus_tag="KRH54_001985"
     CDS             complement(11671..12927)
                     /locus_tag="KRH54_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBG8579642.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(12999..14501)
                     /locus_tag="KRH54_001986"
     CDS             complement(12999..14501)
                     /locus_tag="KRH54_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG8579643.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(14592..>15765)
                     /locus_tag="KRH54_001987"
     CDS             complement(14592..>15765)
                     /locus_tag="KRH54_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8579644.1"
                     /translation="ISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLVSLDIEI
                     LDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHACGHDIHT
                     SCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAIFGLHNH
                     PDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMGIQTIVS
                     RNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLENIVCEIA
                     RGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGEDFSIFME
                     KAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKLKNKI"
BASE COUNT         4950 a         2724 c         1930 g         6161 t
ORIGIN      
        1 attctttaaa aaactattat ataaaataat catgataaaa ttagaaattg gaatttttaa
       61 tatgcactgt acataaatat attttatata aaagtttata ttttcaaaca agtaattttt
      121 cgaaaattgt ctaaattcag taaaaatatg gtaatatata cataagcaat ataaatctat
      181 aaaaaggagt gttacatatg atatctaata aaatgcaaac tttagttgca aatagttctg
      241 ttataagagc tatgtttgaa gaaggaaaga aactttctga tatttatggt gaggaaaatg
      301 tttttgattt tagtatagga aatccaagtg ttgaacctcc agagactatt aaagcagtta
      361 taaatgatat tttaaatgaa gaatctccaa atttagttca tggatatatg aacaactcag
      421 gatatgagga tgtaagagat gctattgctg aacacataaa taaaaaagat ggattaaatc
      481 ttacaagaga aaatttaatt atgacttgtg gtgctgctgg tggtctaaat attattttaa
      541 aaactctttt aaatcctggt gatgaagtta ttgcttttgc accgtatttt ggtgagtata
      601 aaaattatac agaaaactat gatggaaaat taatagaagt tcctacaaat atagagactt
      661 ttgagccaga tttagatgct ctaaaaaatg ctattactcc aaaaacaaga gctttaataa
      721 taaacactcc aaataatcct actggtgtca tatattctga agaattatta aaaaatcttg
      781 gagaattatt ggattcaaaa caaaaagaat ttaatacaag catataccta atttctgatg
      841 agccatatag agaaataatt tatgatggtg ctaaagttcc ttgtgtacta aaatactata
      901 gaaattcatt tataggatac tcttatagta aatcattatc tctacctggt gaacgtatag
      961 gatatattgt tgctaatggg caaatggatg attttgatga tgttatgtct tcattaaatg
     1021 ttgcaaatag aatacttgga tttgtaaatg ctccttctct ttttcaaagg gttattgcaa
     1081 gaagtttaga tgcagaagtt gatgtaaata tttataaaaa gaattttgac cttctatata
     1141 atagtttaat cgatatggga tattcttgtg ttaaacctaa tggtacattc tatttgttcc
     1201 caaaagctcc aattgaagat gataaaaaat tctgcaatga tgcaaaacaa tttaatttat
     1261 tattagtacc aggttcttct tttggttgtc caggtcattt tagagtatca tattgtgtat
     1321 cttatgataa agttaaaagt tctttaccag catttgaaaa attagccaaa ttatataatt
     1381 taaaataact aaatcaaagt aattcaaata attaactacc ttagtataga atacttacat
     1441 tctatattaa ggtagtttat aattttatgt actatatttt atacatacca tattattcca
     1501 atatgcttga atattcttat ctatagtact aatataaatt acaaggaggt gtttgacttg
     1561 aaaaaaggtt atgtttatat aatactgact accttatttt ttagttcaat ggaaattagt
     1621 ctaaaaactg tcactaatga ctttaatccc ttacaaataa ctttatcaag attttttgtt
     1681 ggcggtttag tcttactacc ttttgctgta aaaagattaa gagctcttag tctttctatt
     1741 acaaaatcag acttaaaata ctttgcattt ttaggtttta tgtgtgtagt agtaagtatg
     1801 tcattgtatc aacttgctgt tctaaacaca aaggcatctg tagttgctgt tttgttcagt
     1861 tgtaatccag tctttgtaat gcttttagct tacttaattt tgaaggaaaa aatatatagg
     1921 cacaatgtaa tttcacttat attagaagtt cttggaatta tagttataat aaatccattg
     1981 catacaaaac taactcttag tggaatattc ttaacattat cttctgctat tattttttca
     2041 atgtatactg tatttggtaa aagaaaaacc ttaaaatttg gtggaatagt tgtaacctgc
     2101 tttagcttta tatttggtag tttggaaatg ttgatattgg ttttactcac taaaattaat
     2161 tttatcgcag atatattaaa taaaaataag cttcatgtat ttgcaaatat ccctattttt
     2221 agtggatata ccttacacaa tatgcccatt atgatttatg tattcgtatt tgttacaggt
     2281 gttggttatg cgctttactt tatggcaatg gaagctacat ctactagtct tacatcttta
     2341 gtattcttct ttaaaccagt aatatctcca atactagcat tattaatact taaagaaatt
     2401 atccctataa acatggttat aggaatttta ttgatagtaa ttggttcaat aatttctatt
     2461 atacctacga taattactca aatgcataat aaacacgttg aagccttgta taaggagtag
     2521 taattgataa tctatcatag ttatacataa gacaaaattt attagattta aacgcaatat
     2581 aaaagagatg ctattataat agcatctctt tatttatatt tattttaaat ttattaaaac
     2641 tctatttagc taaattttgc aaaaagctat aagtttgtgg acaagctgat tttaacttac
     2701 tatttgtatc ttgatttaaa tagtaatatg caaatgattc tgcaaaaaat tcttctggat
     2761 atacacctaa gtagtttaca ttacctaaag aacgaccttc tttagcaaat atttgtttga
     2821 attgtgctga tttagatata tcatttaata ctatatggtc tattgcatgt gctgtttcat
     2881 gtaattctaa atttattgaa tcatgccctt tacctttgtt actaaatcct attctaagag
     2941 ctactgtact tccacctaaa cctggaacat catcccaagt ttttccagta ccttcccatc
     3001 ccttaggaac tacacctttt aaataagcat attctttttc atctgttaac tttccactta
     3061 ataattttat ttttaagtta ttttgtttta atgcatttaa atactttcca tctatattag
     3121 ctaatctatt taccatagca ttagcttcgt ttttatcgta atttccagtt ggaaaaacta
     3181 ctatttgact aagtgtgtct ttattttgtt gtatagtagt actatctgca tgtgcactta
     3241 ctggaactga acttattaag aatattgata ttatagctat taataatttt ttacttggtc
     3301 tcatattatt ccctcctttg agttaggccg ggagtaataa atttcaaaat aaaaaagata
     3361 cccaagagta tctttgtata tacaaaaaga aatttttact cttttcggca actggctggc
     3421 atggtttact aaactttagc ccctatagct ttgcgtgact aggtttccct agttttgcct
     3481 catttaattt tatatttata ttatagctta tatcttttta attgtctata tccctttgga
     3541 acttttttct aaaaagttat tattttttta cataaatctt caaaaaaaaa tatcttctaa
     3601 caatattttc attattaaat attgttttag aagatatttt ttaatataac ttatattttt
     3661 ataatgttag ggtctaattt gtatttttat ttcttcttaa tacgataagt cctacaatag
     3721 ctcctataac caaaagtatt tctccaataa ttgtagtact atcccctgtt tttggtggta
     3781 ctggtggatt taccaaagta tcatctttaa ctttattatt tttttcttct gacactgatg
     3841 gctttactac ttctggtatt ggaataatct gtttaacagg agtttctatc atctcttctg
     3901 atggtggtac tggcggattc aatacctctg gtattggcgg atttattata tcttctgatg
     3961 gtggtactgg tggatttata actatagtat tctctgtatc agatgaactt gatggtttcc
     4021 ttggtttatc tgtgcttggt ggtactggtg gatttactat tggttcatct gtatctggag
     4081 gtattggtgg atttactggt ggattttctt gttttagatt ttctatattc ccaatatcta
     4141 ctatcttacc aatatcttca ttaccaattt caaccttaac tttatccttc agtttttcat
     4201 atccacgtgg agccattatc tccactaaat aatatgttcc tgggtttttt acactagatt
     4261 gaattatacc atcttcatca ctaactaaat ccttaactac tatatttcca aatgcatctt
     4321 ctaaattgaa cttagcacct gaaagtacat ttttatctaa agcatccact tttttagctt
     4381 caaaagcata accttgctta gtagtctcta ctcctggagg acttgaacca ttaatctgag
     4441 ccacatttcg tatagtctcc ccaactttta catttgtgaa gtttgcatca aatgtaaccc
     4501 aactttgcac aggttcccct tcttttatct gtttaaattc gtatttattt actaatctta
     4561 ttgtattttt tccatctcca gcttcttttt taagttcaag attatcagaa tattttatat
     4621 tagatatttc tagaactgag tttacttcat ctaaaagatt tacttgtcct gcatcataaa
     4681 ctccataagt tgacatattg attttgtatt taacaatctg gttatcacta ctgtttaaaa
     4741 cttttttatc aacttctttt tttgcgctta ttaatccaga atttttctta aagtttatct
     4801 catcattttt atcaatttca ctattatttg catcaagaac agctctattt atatagctat
     4861 cttcaatact aattaccttt aatgtataag ctataaaaaa ctttgattcc acttcttctt
     4921 tactgagatt aattgtaaat ccagttataa ctttatctac ttgcccactt tcatttcttt
     4981 tttcttctac tttatatgaa atattattgg caattttttc tttactccaa ggagtccagc
     5041 tcccattcat tggaatttct acaactctaa aatcatgccc agcttctcct gaagtagtta
     5101 atctcatgcc ttctggtata ttatctgtta taacaacatt acttattggt gatttatcat
     5161 catttattga aatctgatag tttattactt gaccaacttg agtaatattt gttgctcctg
     5221 aatgtccttt agtaagaaga taatcatctc ctctatcaaa tatacttaca gtagcatcat
     5281 cttcttgtat cttcttacca tcagattcaa tcctagctac attcttatat ttattaccat
     5341 gttttgcatt tttgtcactg attctagcct tatacttaac agtatacgat ttatctatat
     5401 ttccaaaatt aactgaaata gaatttttac tctgttcaat attaaaatca ctaacttctt
     5461 ttccatcact tgttactaca cttacagaac catttatcaa tgtcaatcct tcaggaagct
     5521 catcatacaa aataatgtct tttaactctt cctgcttttc atttacttgt atactgtaat
     5581 ttatatcttt atttttaact ggcataaata cagtagttga tttttttcct tcaacaagct
     5641 ttttaagtgt tcctttagca tctggaacat ccgttttatc tccaggtttt atttctacat
     5701 ctatatctcc acttccactt tctatttttc cagtagtttc aatatgtatt tttgcattag
     5761 tatcttcatc aaccttttta acatatgctt ccaaacctat tttaccttgc attacacttt
     5821 ggccattagg taattctcta aatgtaagtg tatactctcc attactttct gttttatcaa
     5881 agcattcact tgtataatct aaattaactt tattgaatac atctggcacc ttaaaagtta
     5941 ttgaatctcc tgcttgtatt ccttttgatg ttggagtata agttaaatta aatgatattt
     6001 ttttcattga ctccgtttct attgtattgt tattaatttt tacaacaaat ccatagtctt
     6061 cattttcttc attaagttct tcttctaaaa ctttcaataa attttcatct tctaatagat
     6121 tatcttcctc tggagttttt tctgtatttt tattgtctag tggtttttca tcttctaaag
     6181 cttgttctga ctttttatta tctgttgact tatcttcttc ttgtatctgc tctggctttt
     6241 tattatctat tggcttttct ccttctggag tttgctctgg ctttttatta tctgttgact
     6301 tatcttcttc tggggtctgt tctggttttt tattatctgt tggtttttct tcctctgggg
     6361 tctgttctgg ttttttatta tctgttgcct tatctttctc tggagtctgt tctggctttt
     6421 tattgtcgct ttccttctcc tctccagttg atgaattagt tgaaccactt tgaatctgac
     6481 tattctctcc taattctgaa gcataagata cagttggata tattgtagac accaccatac
     6541 ttaaaatcat aattaaacta attaataatg cctttctatt tcctttcttc atgtaaatct
     6601 caccccttta ggttacctat aatttataat ctaatcttcc attaatattg tatataaagt
     6661 aatttttact aaaaaattat acaaaaaaag ctatgaaaat aaataaattt atattcatag
     6721 ccggttgcac cactgactca gtatatccat aaggtgccca ataatagaat atacttcaac
     6781 atctctataa tatttcataa aaacaattaa aattgcttta tttatattaa aaatacaatt
     6841 atatacttct attatattat gcctctatac tattgtctat cctttttaca ttttttacct
     6901 tatgatgaga ttaattaata attaaataaa atccttatct acaacaatta cacaattata
     6961 ttcaggattt atctccttaa ctaattttgt tatatcttct tttaagtcat cttctgacat
     7021 gtttttggaa aattcggaag gattaactac tatatcaaat attaaattct tcttgcttcc
     7081 ttttccaact attctaaaat catgcataga ttttacaaga ctatttttct taagtatttc
     7141 ttcaagttct tttttggctg acataacttc tttatcttca agacatattg ggtccatgtg
     7201 aattactaga tatatatcta atttttcaga tatttctctc tctgcagtat ctataatttc
     7261 atgtatttcc attatatcta tgttggatgg aatttctgca tgtatagatg caatacatct
     7321 accaactcca tagttatgaa taattaaatc atgtgtacct attatatatt tataagataa
     7381 caatagctct ataatagaat ttaccatttc ttcatccggt gcttccccta aaagaggatt
     7441 tatagtatct tttacaagag agaaaccagc ataaagtatc actaatgata ctacgatacc
     7501 aacatatcca tctatgggaa aattagtaaa tctagctaca ataaatgata taactacaca
     7561 acttgaagta aatacatctc ctaaagcatc aagtgaaact gcttttaagg ctgaagaatc
     7621 aattttattt cccataaatt tattaaaacg actcaaccag attttaatca ttatagatgc
     7681 aattaaaagt ataagtggta ttacttcaaa ttttacagga attggattta ctatcctttc
     7741 tatagatgac tttacaaatt gaagtccaac caacatcacc ataaatgcta ctattaatgc
     7801 tgataaatac tctattcttc catgaccaaa agggtgttct ttatcagctg gcttacttgc
     7861 taatttaaaa cctatcatag ttattgctga agatgccata tcagaaagat tattaaatgc
     7921 atcagccatt atagctatac tagatgtgag cattcctatg aataccttta tcacaaataa
     7981 aagtaaattg gataatatac ctactacacc agctacataa ccatacttat ttctaacatc
     8041 tgtattttga acgttttcgc tatctctgat aaatgttttg actaagattt tggaaaacat
     8101 gaaacttacc tccctgccct ttattttatt aacaaatttt ataatctata tctttattta
     8161 tgataacata atttatatat aaaattctta aaaatcactc tacttcagta aaatattatt
     8221 ctatatatct atttagatac gaattttata tttagtaaag tttttatggc ttagtgtctg
     8281 tttaatagat tctatataag tttttacaaa gattatttat tatgataata tcatttttcc
     8341 caagtattac ttagaatatg atttttttgt tttgtctgta catattttaa atttatacta
     8401 tatttatcta tattttataa ccttatgata cactataact ataagttttt aaatggaaag
     8461 gaatgaaata aatcatgttt tttatcagtt atatttacac aaaatcacct attgaatttg
     8521 atactccttt gcaaaaagag gcatacaaaa tattacaaaa attagatatt gactttgaat
     8581 gtgttgacac agatgaagca attacaatgg aagattgtgt ttagattaat aaaaaattaa
     8641 atatgaaaat ggtaatataa aacaacatta aacaaacaaa aaccgcaaag ttacaatata
     8701 tagtactttg cggtttcata ggaattaatt tttactatta aagttcatag ttctactatt
     8761 tttcattgtt ctaagaatta gctttacaaa ttcatttgac aataggatag tggatgcaaa
     8821 tagtattatc tttatccaca tatcaaagtc caaagaaaca gaattaaaga aactctggaa
     8881 cacttgtgta aatattattt gaattatccc tgtaactact attatttgca atgccagttt
     8941 atttttaaag aaatttggta ttgtactatt taaaccaaac tctctacaat ttaatgcatt
     9001 aaaaagggca ctgaatgcaa ataaagaaaa tataacagta ccttgttctt ctgatgtagc
     9061 tcccaatata ttaaaagctg attgagtaaa tacaattgta attataagaa tagcatttat
     9121 aataatattt acaaacatag acctagctat tattccagaa tgtctattta ttggttttct
     9181 tttcaataca taatctctaa caggttctaa tcctaatgct agggctggag gaccatccat
     9241 tattatattt acccataata attgtattgt agtaaatggc atatccttac cagtaagttg
     9301 agatattata gcaattataa atgctactat atttacagta agctgaaact gtataaatct
     9361 ttgaaagttc tcatatatac ctcttcccca ttttattccc tctactatag tactaaagct
     9421 atcatcagtc aatattatat ctgctgcatt tttagatact tctgttcctg aaatacccat
     9481 tgcaattcca acatctgctt gactaagtgc tggggcatca tttataccat cacctgttac
     9541 agctacaact tcgccacttt tttgaagcgc tgaaactatt ctcatttttg tatctggttt
     9601 acttcttgca acaatagata ttcctttgat ttcttctctc aattctttat ctgtcaatac
     9661 atctatatat gttgcttcaa ctgccttttt cccatcattt aataatccta attcatttcc
     9721 aatagctgtt gctgtattta tattatctcc tgtaagcatt tttacatcta cgcctgcatt
     9781 aaatgcttta tcaatggaat ctttgacccc ttctctcaat gggtccacta ttcctacaaa
     9841 tccactaaaa actaaattat cttccttcat ataacttctt gaatttccta ttaaattcaa
     9901 ttcaccttct gaagtcactg caacttctgt tttagaatta ctcatttttt tataagcaaa
     9961 tcctaatgtt ctcatagatt taatttgtaa cttcttaatc tcatctaaaa tacttttttc
    10021 tacttttgga gttattggaa caatattttt tccttgttgt acataagaac atttttttaa
    10081 taatacttct ggagcacctt ttgaaagtaa tatatctgaa tcctcttgac gaattaatgt
    10141 tgacatcttc ttttcttctg aactaaatgg tatttgtgat atcaaatacg tttgttttct
    10201 catttcatta tagttcttat cattatgata taaaagtaat gcacactctg ttgcgcttcc
    10261 tatatattta aatgaattat cttctttttc tatatctgca gttgaattta ctatacagtt
    10321 ttgttcaaaa tagctattac tttgatactc tcctccactt atatatttac catcaacata
    10381 tgctacctct accatcattt tgttttgtgt taaagtccct gttttgtcgg aacatattac
    10441 agaaacagaa cctatagttt cacaggcttc tttctttgtt actaatgcat ttatctttgc
    10501 catcttttgc attgttatag ctaaggtaat atttatcata gttggcaaac cttctggaac
    10561 agttgcaact attaatgcaa tacaaaccat atatgcattt ttagcaggtt ctaatgattg
    10621 taagaaagat aatacactgc ttgtatctaa aattaacata ccttttctct gcatctgtat
    10681 aatcataaac atacataata aagtagctat tacgcctgat attcctgcta ttttagcacc
    10741 caacttccct aatttaattt gaagtggagt ttgctggtct tcatttgata aattctgtgc
    10801 tatcttaccc atctctgtct tatcaccagt atgagtgaca accattatgc ccctaccata
    10861 tgctacaaga gtccctccaa ataccatatt gacttgcttt gcaggtattg catcctgttc
    10921 gattatttct gttttagaat aaactacttc catatctaat acagcatcag cattcttagc
    10981 tacatcatca gattctcctg ttagcatatc ttctctaagt ttcaaattta tagactgtat
    11041 aagtcttcca tctgctggta tcatatcccc agtttctata tatacaatat ctcctggtac
    11101 taaatcattt tttgatattt taattatctt cccatttcta agcaccttta cttctatatt
    11161 ttctgttaat tttgacaggg catgagctgc ttttttagat tttccttcag ttattatgcc
    11221 tatagaaatt ccaataaaaa tagcccctaa aatacctaat gcgtcatgta attctcccac
    11281 aaaagaactt attactgctg ctcctatgag aattaatatc ataggttctg tcaaactttc
    11341 tcccaactcg tcccaaaatg ttctaccttc ttttatagta aattcattta atccatatct
    11401 aagcttcctt tcttctacct cattatcatc aagtccaatt tctggatttg tttttaagta
    11461 ttttaatact tctttggtag gtttattata atacctcata actttatccc ccttgttaat
    11521 tatctataag gaattatatg cttatgatag cttgtatatt acttttcaaa aaaaataagg
    11581 tgttgattaa tcaacacctt atagtggtta tatattatta aattttatgt tacaggcttt
    11641 ataaattgtg agctatacaa atcaaaatat ttaatcttca aaataccctt ctggatataa
    11701 tggaaactta gatataagtt cttgtgcctc tgctttacat tcatctaata cttctttatt
    11761 atcaatattt tctgctacct tattcattat ccctgctatc tgtataactt ctttttcttt
    11821 cagacctctt tgagatattg atgtaagacc tatacgaaca ccactagtta caaatggtga
    11881 ttgtgggtcg tcaggtataa catttttatt aactgttatt cctacatatt caagagcttt
    11941 ttcaaattgt tttcctgtaa gattcttact tcttaagtca accattacta tatgattatc
    12001 agtacctcca cttacaatac taaaaccatg tgaaatcagt tcagaagcta gagtctgagc
    12061 atttactaca acttgctcca ttatcttttt aaatttatct gtacttaaat ataatagtga
    12121 aaaggctttt gctgcaactg tattaagatg tatagaccct aatgttcctg gaaaaacacc
    12181 tttatcgagt ttctttgcat gttctgcttt acaaagtaca ataccacttc ttggtccaca
    12241 gaaagtcttt gtcgtagatg aagaaacaaa atctgcataa ggtacaggac taggtattac
    12301 tttcgctgcg actaatcctg caacatgtgc catatctacc ataaagtaag ctcctacttc
    12361 ttttgcaacc tttgatattc tttcataatc tattaatctt ggatatgaac ttgcccctgc
    12421 aattataagt tttggtctac attcttttgc ctttgcttca agagcatcat aatctatacg
    12481 tccagtattt gggtctaccc cataaaattc atatttataa atgtcatgta aaaaattaac
    12541 tgcgctacca tgtgtcaagt gtccaccttg gtcaagacgc atacttaaaa cagtgtcatt
    12601 tggttttaat attgatgaat atacacaata gttagctgta gaacctgaat acggttgtac
    12661 gtttacatgt tctgctccaa atacttcttt tgcacgtttt attgctaggg tttcaagttt
    12721 atctgcttct tcagaacctg cttgaaaacg agcccctgga tagccttctt ctgttttatt
    12781 tgtaaataca catccactta attcaagtac ttctgttggt gctgtacttt cagaagctat
    12841 catctcaata ttgtgttctt gtctaaccaa ctcatcagct acaatcttgt ataactctgg
    12901 gtctgaaatt tttgttgttt ttaacataat aatcctcctc ttaattaaag aatatatttt
    12961 attagtttaa tcttcattga taaatttatt ataatggtct ataaaacgta agatattcct
    13021 ccaccaggtc ctaatggtat gccaataccc atgaatagta acaatattat tatttctatt
    13081 aatgtaaata ccactgagta tggaatcata ttcgatatga cagtacctat acctatatct
    13141 ttatcatatc ttctggcaaa ggccaatata actggaaaat acggaaaaag tggacttatt
    13201 ggatttgttg aagcatctcc aatacgataa gctatttgag ttaatgctgg attaaagtct
    13261 agtagcataa acatgggaac aaatataggt gctaaaatcg cccattttgc agaagcactt
    13321 cctacaaata aattgataaa tccagatagc aatataaatc ctattatcaa tccatagcta
    13381 ggtattcccg actctgctag taattttgct cctgttattg ataaaattgt tcctaagtta
    13441 ctaagattaa ataaattaat aaactgggat gctataaatg ctaggactat atatccaccc
    13501 atatcactca tactactacc catcatagat actaaatctt tgtcactttt tatcttctta
    13561 cttacttttc catacactaa tcctggagtt aaaaatataa tagtgattat aggaactata
    13621 cctttcatta aaggtgcatt actagatagt atagaaccag tctctggatc tttcataaat
    13681 gcattgtcac ctatacataa ggcaactatc actactacta caactaacaa tgataataaa
    13741 gcgtatttta tacccttatt ttcaagttca ctcaattcct gtatatcctt atcatttgtt
    13801 gaatcattat attttgtaaa acgaggagct ataaattttt cagttacaaa tgctcctgca
    13861 aaagttaaaa ctatcgctga tataaaagca aaataaagat tcattgctgg attgccttgg
    13921 taagatgggt ccatcatttg agctgctggt attgtaaatc cagctaacaa tacatctgta
    13981 gtactaagca taacatttgc tgcaaatccc cctgcaacac ctgcaaaagc tgcaaacata
    14041 cctacaagag gatgtcttcc aattcctaaa aacacaagtg ctgcaagtgg tggtaatatt
    14101 ataaatccta catctgctgc tgtatgtgat aacatacctg caaatacgat tgctacagtt
    14161 acaagttttt gtggaacttt acttataccc tttttcatta acacttccat aaatccagat
    14221 ttctcagcaa cacctgctcc aagcattgta accagtacta atcctaaagg agcaaatgac
    14281 tggaagtttg taactatgct acctaaatat gattgaatct gctctttctc tagtagattt
    14341 actaccttta caacttcttt tgtagaagga tgtatttctt ctactcctaa attagatata
    14401 actgctgaca atattacaac aattatacat agtaataaaa atatggttac tgggtctgga
    14461 agtttatttc ctgctctctc tacaacattt agtgccctca tcaataatcc actatctttg
    14521 tttttttcct gtgaattaat tttcctcaaa ctaatacccc ctcataaact ttatctccag
    14581 aatgctttat cttaaatttt attttttaat ttatccatat aatttataac agactgacat
    14641 agtacatttg tacctaccaa tatagaattt ttatcagctt taaatttagg attatgccac
    14701 tgatatatac aatcttgttc tttgttgcct acccctaacc agtaaaaaaa tcctggggct
    14761 ttttccatga atatagaaaa atcttctcct cctgtagaag gtattggatc aactatagca
    14821 tcttctgaat acaaatcaca cacagactta caagctattg cgtacatctc tttactattt
    14881 ataacagctg gtaaatcaaa taaataatct agctttgcag ttgcttgata tccacgagct
    14941 atctcacaaa ctatattttc taaattttct gatattttct ttcgtacttc attactaaag
    15001 cttctacatg tgcctgtcat atctaccttt tctgaaatta cattatttgc agtccctcca
    15061 tttatagtac ctattgatat aacagctgat tctaatggac ttatgtttct gcttactatg
    15121 gtttgaatac ccattataat tgcacttgat gctactatag ggtctattgt tctgtttggt
    15181 atacctccgt gacctccaaa tccatttact tctatcttaa tagtgtctac tgctgccatt
    15241 aacccaccta acttaacacc tatttttcca caaggtatat ctggatgatt atgaagtcca
    15301 aatatagcat caacttttgg attttccatt actcctcgtt ccactagcat cttcgcacct
    15361 ttattgactt cttcggctgg ttgaaatata aactttacat ttccatgaag actatctcta
    15421 atatgagaca atacatatgc acatcctaca aggcaagacg tgtgtatatc atgcccacat
    15481 gcatgcatta taccatcata tctggattta tacttaatat caacctcttc atatattggc
    15541 aatgcatcaa tatctcctct tagtgctact gtgggaccat catacttccc ttttaataat
    15601 ccaacaactc ctgtttccat tcctaaatct aatatttcta tgtctaaact tactaataaa
    15661 tcttttatag cttttgtagt cctaaattct ttaaaactta attcggggtg catatgaata
    15721 tctgtaaaaa aatctaaaat atcttcttta tacttttctg agatt
//
LOCUS       DAEOSP010000080        15697 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.080, whole genome shotgun sequence.
ACCESSION   DAEOSP010000080 DAEOSP010000000
VERSION     DAEOSP010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15697
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.080"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            155..1372
                     /locus_tag="KRH54_001988"
     CDS             155..1372
                     /locus_tag="KRH54_001988"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBG8579645.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETRATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1467..1847
                     /locus_tag="KRH54_001989"
     CDS             1467..1847
                     /locus_tag="KRH54_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG8579646.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2131..2613
                     /locus_tag="KRH54_001990"
     CDS             2131..2613
                     /locus_tag="KRH54_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG8579647.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2634..3449
                     /locus_tag="KRH54_001991"
     CDS             2634..3449
                     /locus_tag="KRH54_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG8579648.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3500..5050
                     /locus_tag="KRH54_001992"
     CDS             3500..5050
                     /locus_tag="KRH54_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG8579649.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPFLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5147..6499
                     /locus_tag="KRH54_001993"
     CDS             5147..6499
                     /locus_tag="KRH54_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG8579650.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6630..7388
                     /locus_tag="KRH54_001994"
     CDS             6630..7388
                     /locus_tag="KRH54_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8579651.1"
                     /translation="MNIINKIFWEEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7435..8154
                     /locus_tag="KRH54_001995"
     CDS             7435..8154
                     /locus_tag="KRH54_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBG8579652.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8278..9678)
                     /locus_tag="KRH54_001996"
     CDS             complement(8278..9678)
                     /locus_tag="KRH54_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBG8579653.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9682..10590)
                     /locus_tag="KRH54_001997"
     CDS             complement(9682..10590)
                     /locus_tag="KRH54_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8579654.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10851..11498
                     /locus_tag="KRH54_001998"
     CDS             10851..11498
                     /locus_tag="KRH54_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG8579655.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11823..14462
                     /locus_tag="KRH54_001999"
     CDS             11823..14462
                     /locus_tag="KRH54_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG8579656.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHIEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMNTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14520..15623)
                     /locus_tag="KRH54_002000"
     CDS             complement(14520..15623)
                     /locus_tag="KRH54_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBG8579657.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
BASE COUNT         5905 a         1896 c         2744 g         5152 t
ORIGIN      
        1 atatgtttat tgtatgcaat tgttgtattt ttaggttttt tgtgattaat taaaaaatta
       61 taaaaatcgt ttacatgttg atttttaaaa aaaaaagata tagaattata tagaaagtaa
      121 taaacaaaaa ttggatttac gggagtggaa tgaaatgatg aatgttacct taacagatgt
      181 gaaagaggct agagagacca ttaagaacat agtaaaaaga actgacttac ttgaaagtgt
      241 taaacttagc gaaaaaacag gagcaaatgt gttctataaa tgtgagaatc ttcaaaaaac
      301 aggttctttt aaattaagag gagcttgtaa caaaatagcg agtttaacag atgaagaaaa
      361 ggcaaatgga gttatagctt caagtgcagg gaatcatgct caaggagttg cattgggagc
      421 aaaaatgact ggtataaaat ctacaatagt aatgccagca actgcgcctt tagcaaaagt
      481 tagtgcaact aaaggttatg gagcagaagt agttttaaat ggtgcggttt atgatgatgc
      541 atatgcaaaa gctgttgaaa tacaaaaaga aacaagagca actttcttac atccattcaa
      601 tgataagtat gttatagcag gacaaggaac tatatctctt gaaattttcg aacaattaga
      661 caataaagtt gatactatac tatgcccagt tggtggagga ggaatcatct ctggtgttgc
      721 tgttgcagca aaagcattaa atcctaatgt aaaaatagtc ggtgttcaaa ctgcaaatat
      781 accttcaatg aaagagtcaa taaaaaatgg aaaagtaaca acagcattta atgatactac
      841 aatagctgat ggtatagctg ttaaaactcc aggagattta acttttgaaa taataaatga
      901 attggttgat gaaatagtag tggttgaaga gacagaaatt gcagaatcaa tattatttat
      961 gatggaaagt caaaaaatag tttcagaagg tgctggtgct gtatgtacag ctgctatatt
     1021 aagtggtaag tatgtaccag caaaagatga aaatgtagtt tgtataatat ctggtggtaa
     1081 tatagacata aatacgttat atagaataat aggtgttgct ttagctaaag aaggaagaag
     1141 atattcattt agtacaatta tggaagataa gccaggaaac tttgctgagt taactagaat
     1201 aataagtgag aatggtggaa acattttaag tgctaatcaa ggtaaattat cagcagggga
     1261 agctctagga aaacaaagtg cggaatttat attagagaca atagattatg accatatagc
     1321 tagaataaag aaagctatag aagaaaaagg ttttaaaatt atagaattat aataaaaagt
     1381 gtatataata tacaagaaaa atagtataat agtgtatgaa gatacatttt atgtgtatta
     1441 gattaacgaa ttaggaggat tttaaaatga aacatgaagt aatacatact aatgatgcac
     1501 cagcagcttt aggaccatat tctcaagcta taaaagctgg aaacttgtta tttgtatctg
     1561 gacaagttcc tcttgtacca gagactatgg aagtagttga aggagatgta caagctcaaa
     1621 ctgctcaatc tttaaaaaat cttaaagcta tattagctga aagtggagct gatttttcaa
     1681 atgtagttaa gactacagta tttataaaag atatgaatga atttggggct ataaacgaag
     1741 tttatgctga atattttgga gaaaataaac cagcaagagc ttgtgtagaa gttgctagat
     1801 taccaaaaga cgttaaagtt gaaatagaag taatagctgt attataaaaa tacaatattt
     1861 ttaatttgaa aaggctgaaa aactatattc tagtgattca gcctttttat aaaaaaataa
     1921 atcacaataa agaacatgat aaatattgtc gaattaaaaa ataaaattaa aataaatatt
     1981 gcaatatttt tgagaaggtt ttataatgag tatataattt gaaacgtgta actattaaat
     2041 taggcatgag ttcttggata tttttatcct gaggattcat gccttttttg tattgaaatt
     2101 caaaaatcta tttaataagg aggaagtact gtgttaaatt ttttcaagaa aaataaaagc
     2161 tataagttac atgcagtagt gagtggtaac agcattaata ttgagaaagt aaacgattct
     2221 gtgttttcaa aaaagttaat gggagatgga gttgcaatta ttcctaatag taatgttgtt
     2281 gtagcccctt gtaatggtaa agtaactgtg cttacagaat caaagcatgc ctttggtatg
     2341 gtctcagatg agggtgttga aatattagtc cacataggaa ttgatacagt tagtttacag
     2401 ggtgaggggt ttaaaaatga agtgtctcaa ggagatactg ttaaaaaagg tagtccaata
     2461 ataagttttg aaagagaaaa aataaattct cagggaattg attgtacaac aataataatt
     2521 gtattaaatc atagcgaatt ttcagaaata aattgcatgg tagaaaatga agttgttgca
     2581 gggcaagata ctgttattga aattatgaaa taataggaag ggattaattc aatatgagag
     2641 tagtaaaaat ctttaataat aatgcattat ctacagtaac taatgatagg aaagaagcta
     2701 ttttactagg tcttggtata ggttttaata aacgtccagg agataaagtt aatgaagata
     2761 aaatagaaaa gatttattat gttcaagacc acatgcaaac aaaattttta gaattattaa
     2821 aaaatgttag tccagaagtg atggatgctt cacaacaaat tataagtctt ataccaggaa
     2881 atgagggaaa atttaataat aaagggatat tatctttagt ggaacatatt agttttgcaa
     2941 tagatagaat gaaaaataat gtttttttac ctaatttaat gcttttggat ataaagatga
     3001 tgtattcaaa agagtttgaa ttaggagtaa aagctttaga aattatatat agagtatgtc
     3061 atatccattt gcctgaagat gaagcaggtt acatagcttt acattttgtc aatttacaat
     3121 caaatgataa tttggcatat gatactttaa aatttgtaaa aggtagtatt gatttgatta
     3181 aagaatgtta tggattagaa ttagacgaat cgagtttaag tacattacgt tttagaactc
     3241 atcttaaatt tcttgctcaa agaattttta agaatgaaat ttgtcaggat gataaaatga
     3301 ttgaaatgta tgattattta attaacaatc atcctaaaaa taaagaatat ttagaaaagt
     3361 taaatgtata tattgaaaag gagtttaggt ataaattaga taaacctgaa aaaatttatc
     3421 tactactaca cctaacaaaa atcttataag aatgagatga attagtaata gaaatatata
     3481 attttagggg gggataagat tgaaagaaaa actacttgga ggactggaaa catttgcaaa
     3541 ggcaatggtt cagccattaa tgtatttatc gagtgcagga atattaatgg tggtaggtgt
     3601 actacttaca aatgatatgt tattaggagt acttccattt tttaaatggg agccaattca
     3661 aatatttgga agcctaattt ataactgtat aatggctatt attaataatc ttgctgttat
     3721 atttgctata ggtattcctg ctgcactagc aaaaaaagag aagcacaaag cagcgttaat
     3781 aggatttatg agttatataa tataccttac agcttccaac actatgttaa catcactagg
     3841 tcaactagca aaagaaagca agatgcttgg actgataggg actggacaag ctactatttt
     3901 aggtattcaa gttgtagata caagtgtatt tggtggaatt attttagggt gtatagctgg
     3961 atatgtattt aatcgtacaa gcaacaaaac atttaaagga gctttacaga tatactcggg
     4021 agcaaatttt agttttatat gcatgttctt tgtatccatt ttaattggag ttgtgagtaa
     4081 ctttatatgg ccattagctc aacaaggaat agcttctctt gcaaatgtca tcaaagatac
     4141 aggtacattt ggtttattct tatatggttt tttagagaga atattagtac caactggact
     4201 tcatcattta atctatacac catttcagtt ttctgattta ggaggagtgc ttaaagttgg
     4261 tgcagataca gtttcaggag cctatgctat agttatgaca gaaatgaatt taccagttga
     4321 gcgtttctca gatagtatat actacatggc aactggattt acaaagatgt ttggttatat
     4381 aggtatagga gctgccttta tttatactgc atataagcaa aataagaaga aagttaaggc
     4441 aacgattatt ccactaataa ttacggcagt attagcctct gtaacagaac cattagactt
     4501 tatgtttgta ttctgtgcac catttttatt ctttgtacat gcagttatag ctggtctttt
     4561 tatagcttta ttaaaaatat ttgatgttac tgcattatgt ggagggaact tattagtatc
     4621 aattataatg aatactacag taggcgtaga aaaaacacac tggccagtaa tgatgatatt
     4681 aggacttgca caaattattg tatattttgt agtatttagt tttttaatta agaaatttaa
     4741 ttataaaact ccaggtcgtg aagatgagag tacagctaca ggcgaagaac aatcaaaaca
     4801 acttaattta gatgcaggga tagaaaatat aattgatggg ttaggcggaa aagaaaatat
     4861 taatacagta gaaaattgca tcactagatt acgtgttaat gtaaaagatg aaagtaagat
     4921 taatgaagat ataattaatt taacgccaaa tagcgggatt gtaaggaaag gaaaagatat
     4981 acaaataatt tttggacttc atgtgcatga ggtaagacgt gcagtagaag attttttaga
     5041 agcttattaa agatatatat ttatatagca agggtaaata acagctttaa gtgctgttta
     5101 ctatttatta aagatacata taatttatag aggaggaata attattatga ttattactat
     5161 tgttggtgga ggaagcacat ttactccagg tattgttaag tcaattgcat taagagaaaa
     5221 agagttagag cttgaggaaa ttcgcttatt tgacattgat aaagaacgcc aagataaagt
     5281 agctgtagtt gtcaactgga tactaaaaga agatgttaaa acagatgtta aactaactgt
     5341 aactaattgt gaaaaagaag cgtatacaga tgctacattt atatttgcac aaatgcgtgt
     5401 tggtaaatat gaaatgcgtg agcaggatga aaaaattcca ttgaaatatg gttgtgtagg
     5461 tcaagaaact tgtggttgtg gagggatggc atacggactg cgtacaatac aaccaatgat
     5521 aaaggttatt gatgatgtag agaaatatgc taaaaaagat tattggatat taaattactc
     5581 taatcctgct gcaatcgttt ctgaagcttg tcgtaagtta agaccaaatg caaggattat
     5641 aaatatctgt gatatgccaa tagctattat agatatgata gcggggtcat taaatatcaa
     5701 tgacgttcat aatatacgct atgattattt tggtctaaat cattttggct ggtttacttc
     5761 aatagattat aaacatagag acttaatgga agagattaag gaatacataa aagaaaataa
     5821 aatattatta ccaaaatctt atatagaaaa cttaaataaa agtcgtagta caggaaatcg
     5881 ccatgctgta agtagctgga tacatgtttg ggaatctgtt tatacaatgt tagaaagctt
     5941 cccaaaatat ctgccaaaca cttatttaac atactatctt ttaagtaaag aaacagtgga
     6001 acattcaaac ataaatcgta ctcgtgcaaa tgaggttatg gaaagccgtg aaaaagactt
     6061 atttgaaggt gtgagacatt acttagaaac tggagaaatt tctgaaaaag cattttatgc
     6121 aggttcacat ggagattgga ttagtgattt ggcagtttca attaaaaatg atacacgtag
     6181 tcgtttttta gttataactg aaaatcgtgg agctatacca aacatgccat atgatgcaat
     6241 ggtagaaatt ccagcatata ttggtaaaga tggtccagaa gttattgctc gaggtccaat
     6301 acctctattt caacaaggat taatgatgca acaacttaac agtgaaaaat tgatagtaga
     6361 agcttatata gaaaatagtt atgaaaaagc tttacaagca ttcacattaa ataaaactat
     6421 tccaagtatg aatgttgcta aagaaatttt ggatgaaatg atagaagtta ataaggaata
     6481 ctggccagaa ttaagatagt aggataaagg aaagggtttt tatttaataa ttagatatgt
     6541 tttcagagtc ttttatcaaa atgtattcac aaacatatgg caatatatta aaataaagag
     6601 tgatattttt agaagaatgg ggtgatgcaa tgaacattat aaataaaatt ttttgggaag
     6661 aagataggat tggcatgatt acaaataatt tagaggcaga tacacatagc cactgtatgt
     6721 tacaactgtt cttagggata gaagacagca ttgaaataac agtcaatgaa aaattagtta
     6781 aatgtaattg tatcatagta gataaaaata tctctcattc attttcagca agaaaaaaag
     6841 tttattactc tgctattatt gaacctacat ctatatatgc agaacagctt acctcaaaaa
     6901 tgaatgattt tggctactgg atatgtgaca atgatggatt agagaaatta cgtcaacaag
     6961 gaactttttt gatagataac tctagtaaag aacaatattt aagatttatg gaaatgctta
     7021 ataactattt gaatatacct ataactctca aacattatga tgacagaatt actgaattgc
     7081 tcaatttatt acgtacttgt aattgtgata atcatactat ttcaagtttt gcagataaag
     7141 tgtccctatc agcaagtaga ttatcacatc tgtttaaaga acaaataggg atacctttaa
     7201 aaagctatat cttatttcat caaatggagt gtgcttttag agaattactt tctgggaaaa
     7261 atgttaccga agcttctatg actgctggat tcgatactcc atcacacttt gcagggacag
     7321 ttaagcgaat gatgggaatg cctgtttctt tatctttgaa agatagcgaa tttttgaaag
     7381 tatattaaat attctcgtta tataatgaag ttgtactaag aaaggggctt tgttatgaat
     7441 aaagaatatt tagaggaaac aaaaatgtta aattataatg aaccacaact taagcttttg
     7501 gtatcatcaa agaattggtt ggaattagat gattttcata aaataaaatc tatttatgag
     7561 tttgtacaga atgatatttt atttggatat aatgcttctg atatgttaag cgcaacacag
     7621 gtattaaatg atggtatggg acaatgcaat acaaaagcta ctcttttaat ggcattatta
     7681 cgtgcagtga atatcccttg taggcttcat gcttttgatg taacaaaaga ttttcaacga
     7741 ggagctacat ccaaattgat ttcactatta gcaccaaaat atattcttca tacttgggta
     7801 gaggtatttt atcaagatag gtggattgct cttgagggtg tcataactga taaaaaatat
     7861 ttagaagcaa ttcagaagaa attttttaat catggtggaa catttaaaaa gtatgcaata
     7921 gctacaaatg atttgaaaaa tacatctatt gattgggatg gtaaagatac ttttatccaa
     7981 aaagaagcta tcgtgtatga ttatggtatt tttccatcac cagacgtatt tttttctact
     8041 cattcacaac atatgtcaaa attaaaaaat ttcatatatg tacatcttat cagaaaaata
     8101 atgacaaaaa atgtatgtaa ggctagaaac aattatattg ataaaaacga atgagtgttt
     8161 aagaggtaac tatataggaa atatatagaa taattaaaac tgtactggtt aaaatgaata
     8221 aaaaggagtt atcacagagc aaacttaaat tgttcatagg ataactcctt ttctgtatta
     8281 acttctaata atttgatgtc tttttataaa acatatagat acaattaaac cagtaaatgc
     8341 aagagtagct gctattgaaa atgcaacatc tattccatga atgttagaca gcatgtttga
     8401 actcatatga gctttcttag tagcacttgt cattatagta ataagtatag cagaacctat
     8461 agctccagat atctgacgga gtgtgttatt aattgctgtg gcatgagata taagttctct
     8521 atctaatgtt ttaattcccc atgtagttaa tggcattaaa accattgaaa taccaatcat
     8581 acgaatacaa tacatcaatg acacatgaat taaagatgta tctctgccta aaaaagcgaa
     8641 ggataaagtt cctaaaaaca aacatcctgt tcctaatatt gatagagcat gaggtccata
     8701 tttatctaaa tgacgtcctg caattggatt gagtacaacc atcaatatag caccaggtaa
     8761 cattaatgaa cctgaagata atgcagaaaa tcctcttaca gattgaacgt acagaggaat
     8821 cattaatgta gcagacatca ttgaggcata tacaatcata attaatattg tagaaataag
     8881 aaatggttta tttttaaaaa ccctcaattc taaaaaaggt tctttagatt ttaattgacg
     8941 caatgtaaat aaaattaatg acataattcc tattaatatt ggtaaatatg tggcaatatt
     9001 agtccaccca taattacctt gattagtaaa tccaattaat aaaccaccaa aacctagaga
     9061 tgatagtact attgaaggta tatctaattt tagtttttga gtttccccaa catttcttaa
     9121 tagaataaat gatagtataa catctaatat agcaataggg gaaagaaaca gaaataaact
     9181 atgccaacca aatgaatcta ctaaccatcc tgataaagtt ggacctattg ctggagcaaa
     9241 tcctactgta ataccaacta aactcatagc tgcaccacgt ttttcctcag ggcataaata
     9301 gagtataata acttgtaaaa gtggcattag agaaccagaa cccattgcct gcaacacacg
     9361 agcaatcaac atagttgaaa aattattaga aaaaagtgca ataatacatc ctatagaaaa
     9421 gatagacatt gatgtaataa ataactttct agtactaaat cgatttatta aataagctgt
     9481 tgtgggtaca ataatacctg caataagtag ataaattgtt gttaaccact gaccaatatc
     9541 agcagtaatt ttaaaatctg acattatatt aggtaatgct gttgacaata gtgtttgact
     9601 gagagaactt ataaaagcac cagtcattac aattgctatt attaaatttc cttttttatt
     9661 gttactttgg gtttccataa tttataacgt ctcctttttt aattgatttt taatgctgtt
     9721 caataaaagt acttttggtc ttaaagtgtc ttctttttct aaatatgcaa tcattgcatc
     9781 cataattaaa gaatcatatt gttgattttt tccttcgacg tataattttt ttattgcttc
     9841 tagtaattta ggattttcaa ggagagaagt atcagttttg attagttctt ctatatcttg
     9901 tatcgtttta aaaaatagcg atttattatg aagggaaaag taaacttcat cttcttgttt
     9961 atgtaaaaac attgattttt caattaaagg atttagatta tccatatttt ctacaactaa
    10021 atctagaatt tgtatagtaa atttggcaat aaagtataca tttatttctc ttttttgtac
    10081 atgaataatg tatataaact gatgaattag agcatccaat aagaaaacta aatcaaatat
    10141 aattttttct attttatcac catagttatc aaataagctt agtttgtatt cattcaataa
    10201 gttagctctt gattttgttt gcaatttact tgtagcctta cgttgctctt cagaaaagtt
    10261 ttctcttata taggtattta tggtagttct agaaaaaata tttttccata cagcaattat
    10321 ctttctgttt aacttttctg atgatggaat gtcattattt tcatttatgg aatgtataat
    10381 ttctaaaaac ttttcatcat aataattgat tatttcgcag ataaaacttt ccttagtttc
    10441 aaaatgcttg taaaaagtag ctttagacat tttacactct tttacaacat ccgatattga
    10501 agtattttga aaaccttttt ggtcaaaaat tttttgtgca atagttataa tgagcctttg
    10561 tttttctgtc actttattat ctcctttcac gcaaaaacgt ttagtactat atagtaccta
    10621 caagttttga taaatatatt atacatcatt tggaaaaaaa atcaatatat agactgtaat
    10681 atgaaatttt tgaaaaattt gttgttattt ttagtcgtat aaaaaagaaa cttaagagtt
    10741 tcttaagaat tgtttaaaga agttgcaata aattagtggt atgattatag tatgaaagag
    10801 aatgtcttga aatttttctg tatcaaattc caattatgga ggtgtagtat atgaaaaaga
    10861 aaatttgtgt agcaataatt tttgctctat ttatagtttt tgtagaaatg tttattgata
    10921 gctctagcga aggaagtaaa attatgacag ctgaattaag taaatacagg aaaggcgtaa
    10981 atgtattatc aataaacaat attatagtag cgaataaaaa atatagtttg cccaaagatt
    11041 attctcctca ggaaagcagt gaagcgagag atgcttttta taaaatgaat aaagatgctc
    11101 aaaagtcagg attaaattta aaagctttta gtacatatag aagttatgaa tatcaagata
    11161 gattatttaa gtcatatgta aaagaacatg gagaaaaaga agcaaataga ttttcagcta
    11221 aacctggaga gagtgagcat caaactggac ttgcttttga tattggagga gatgaccagt
    11281 cttgttgggc aaataaaaag ttcaacaata caaaagaagc taagtggttg tatgaaaatg
    11341 catataaata tggttttata cttagatatc cagaaggtaa agagaatata acaggatata
    11401 tgtatgaatc ttggcactat agatatgttg gaactgaaca tagcaaaaac tttgcaatga
    11461 ataatttgac tttagaagaa tatttacata taaattaaga gatggtttgt taaaattagg
    11521 ttaaaataaa aaataaccta gactattagc tgtgtaaagt gatataatgt aaatacaatt
    11581 taagataact actcgaaggg gagtagctta cacgaaagtg gtaattaatc gtcaagacag
    11641 tagaaatact cggttaatta gcaagggatt ccttgatagc aagaccttca aaataagcat
    11701 taggtatgtt tgttttgggg tcttttttta ttcaaggaat ttttatttaa aaaaatttaa
    11761 atttcaagta tacataagag cccccaaaca taaaataaat ttattgaaga ggagggcaac
    11821 aaatgtctta caaaagtact tatcaagaag taattaaaaa tttagactca agtttatctg
    11881 gtttgagtaa agaaagagca gaagagttgt tagaaaaaaa tggagctaat gaattaaaag
    11941 aagcagataa ggttcctact tataagcttt tcctagaaag ttttaaagac ccattagtaa
    12001 taattctatt gatagctgct ttagtacaaa tatttttagg tgaaacagtg gagtctataa
    12061 taatttttgc agttattatt ataaactctg ttctgggtgt tgtacaaaca aaaaaagcag
    12121 aaagttcatt agaaagtttg aaaaatttat ctgcaccaaa cgcaaaggta ataagagaca
    12181 ataaaaaaat gacaattcca gcaaaaaatc tagtagttgg agatattgta ttcttagaag
    12241 ctggcgatta cataccagca gatggaaggc ttatagaagc acaatctctc aaggttgtag
    12301 aagggatgtt aactggagaa tcagaaccag ttttaaagca tatagaaaaa ataagtgaag
    12361 atgtaagttt aggtgaccag aaaaatatgg tatttagtgg tgctgtagtt gtttatggta
    12421 gaggttcttt tgtagtgact ggaacaggta tgaatactga gatgggtaaa atagctgggc
    12481 ttttagaaag tgctgaaaat aaacttacac cattgcaaca aaaaatagag gattttagta
    12541 aaaaacttgg tgtaggtatt gtaatacttg ctcttttaat atttataatt caagtagcaa
    12601 gaaattactt tatggctgga aatggagaaa tgacacaaat tattttagat tcattcatgt
    12661 tctctgtagc agtagcagta gcagctatac cagaagctct atcatcaata gtcactatag
    12721 tattggctgt tggaacaaat agtatggcaa aaaaacaagc cataataaga aaattaccag
    12781 cagtagaaac tttaggttca acaagtgtaa tatgtactga taaaacaggt actcttacac
    12841 aaaataaaat gacagtagtt gattattata tgtatggaac taataatgat gagttagata
    12901 aagaaaaagt aaactattct tatcatgcta aattattgac tatggtttct acattgtgta
    12961 atgactctca tataactagt gatggtaaag aagttggtga cccaacagaa gtggctttaa
    13021 taaattattc aagtaaaaat gatttagatt atgataaatt aagaagtaag tatatcagaa
    13081 taaatgaaat accatttgac tcagacagaa agcttatgtc tactgtaaat agtatatatg
    13141 gagattatat aatgtttaca aaaggtgctc cagatattgt gtttagtaga tgtaaatatg
    13201 ctttgaaaaa tggttcaaaa gtagatataa cagatgagat aatagaagaa tataaacata
    13261 aaaatgaaga gttctcaaat agagcactta gagtattagc ttttgcaata aaagatgtcc
    13321 cagaagaaga ttttatacct tcaattgatg acgaacatga catgacattg gttggaataa
    13381 tggcaatgat agacccacca agagaagaag taatggatgc agtaaaagag gcaaagagtg
    13441 ctggtataaa aactgttatg ataactggtg accataaaac aacagcagca gcaattgcaa
    13501 aagagattgg tataatggaa gaaggagact tggctcttac tggaaaagag cttgatgctt
    13561 tgagtgaaga acaactatat gaaaaactag aaaatataag tgtatatgcc agagtctcgc
    13621 cagaaaataa aattcgtata gtaaaagcat ggcaacataa gaataatata acagcaatga
    13681 ctggtgatgg tgttaatgat gcacctgcat taaaacaagc agatatagga atcggtatgg
    13741 gaagtggtac tgatgtagca aaagatgctt ctgctatggt attggttgat gataactttg
    13801 caagtatagt aaatgctgtt gaagttggta gaacagtata cagtaatata ataaaatcaa
    13861 ttacttatct atttgcagga aacttaggag ctatagtatc catagtattt gcagtgtttg
    13921 caggttggtc aaatccattt acagctttac aacttttgtt tataaactta gtaaatgact
    13981 ctttaccagc aattgcatta gggtttgaaa agtcagaaaa aaatataatg aataaacctc
    14041 ctagacaccc ggatgaaagt atactagcag gaggaacatt gaaggcagtt gctataagag
    14101 gttgtataat aggtgtagtt acaataattg cacaatatat aggattacaa gtatctccag
    14161 aacttggagt agcaatggca ttttcaacat tgatattagc tagaataatg caaacattac
    14221 ctgcacgttc taataatcaa acagtaataa aattaggttt tttcagcaat aaatacgtta
    14281 taggagcagt tttagtctgt cttgggcttt actctttaac actaatacca gtgctaagag
    14341 gaatattctc tataccaaca acgtttggat tgtaccagtt ggggatttgc ctagggttag
    14401 cattaatttc atcaatatta atggaactta acaaattaat aaatagaagt gaagtaaaat
    14461 aatcaaaaaa ttaaagggca tattgatttt aacaatatgc cttttataaa tagtctaatt
    14521 tattctatat tatcgattat ttggaatgct tttataactt caggtaattc tttttcttca
    14581 ggcattctaa atcttatgaa atttttatca agtccagtaa aatcacttcc agatataact
    14641 cttattttta atttagcaaa ctcttgttgt aaatctatgt ccttgttttt atgttcaact
    14701 gtcataattg atacagtatc actagtttca gctatattaa ggtttttcca tggaattaat
    14761 aattgatttt ttatatcttt tgttttttct tttaattctt ctataaattg aacatcatct
    14821 aataaatttg ctgcaatact acgagatatt tcagacactt cgtaaggagt agaaatcttt
    14881 ttaatagggc taacaagttg ttctggaaga actgcatatc ctgctcttag tcctgctaaa
    14941 ccaaatcctt ttgaaaatgt ttttaaagca attacattat cataattatt tagaagcttg
    15001 acagctgaat tttcttttgg catgtattca ccataagctt catctaccat aactgctata
    15061 tcatatttag ctgcttcctt tactatattt tcaattgatg ataaaggtat aatttgtcca
    15121 gtaggattgt ttggattgtc tatgtaaatt actttatatt ctggatttat tttttcaata
    15181 aattcttttt cattaaactt gaaattatcc tctttcttta aaagaacata atcataagtt
    15241 gcaccatgca tttttatatc tgtctcatat tctgagaact gaggtgaata tcctaaaact
    15301 ttatctccct tttcaataaa tagtctattt aaaaggtaaa taacatgtat agaaccatct
    15361 gccaaacaaa ttctgtcagt atctaatgct ataaaatctt tccaataatc aactatatta
    15421 tcttttaaag caattgaatg aggatatcct cttatcatat caaattttaa agcattaaaa
    15481 gcttctacag ctttgtttga gtaagacacg gtatttatac cttcaccaca gtctatctcg
    15541 aatacatctt ctgaatgtcc agattctatt gcgtaacttt ttttcaactg catttcaata
    15601 gctttgttaa tttttagttt catactttat cactccttat ttagtcaata ataaatgtta
    15661 taaaagttgc tagatttaaa atattatgaa aatatat
//
LOCUS       DAEOSP010000081        15679 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.081, whole genome shotgun sequence.
ACCESSION   DAEOSP010000081 DAEOSP010000000
VERSION     DAEOSP010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15679
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.081"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            310..1701
                     /locus_tag="KRH54_002001"
     CDS             310..1701
                     /locus_tag="KRH54_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG8579658.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            1954..3747
                     /locus_tag="KRH54_002002"
     CDS             1954..3747
                     /locus_tag="KRH54_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG8579659.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDGIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(3828..5210)
                     /locus_tag="KRH54_002003"
     CDS             complement(3828..5210)
                     /locus_tag="KRH54_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG8579660.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPINGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            5468..5815
                     /locus_tag="KRH54_002004"
     CDS             5468..5815
                     /locus_tag="KRH54_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG8579661.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            5833..6867
                     /locus_tag="KRH54_002005"
     CDS             5833..6867
                     /locus_tag="KRH54_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579662.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(6916..7965)
                     /locus_tag="KRH54_002006"
     CDS             complement(6916..7965)
                     /locus_tag="KRH54_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8579663.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            8240..9775
                     /locus_tag="KRH54_002007"
     CDS             8240..9775
                     /locus_tag="KRH54_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579664.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(9960..10646)
                     /locus_tag="KRH54_002008"
     CDS             complement(9960..10646)
                     /locus_tag="KRH54_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBG8579665.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            10771..12153
                     /locus_tag="KRH54_002009"
     CDS             10771..12153
                     /locus_tag="KRH54_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG8579666.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            12555..13823
                     /gene="eam"
                     /locus_tag="KRH54_002010"
     CDS             12555..13823
                     /gene="eam"
                     /locus_tag="KRH54_002010"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBG8579667.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPIYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(13916..14272)
                     /locus_tag="KRH54_002011"
     CDS             complement(13916..14272)
                     /locus_tag="KRH54_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579668.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(14423..15517)
                     /locus_tag="KRH54_002012"
     CDS             complement(14423..15517)
                     /locus_tag="KRH54_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG8579669.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         6093 a         1926 c         2511 g         5149 t
ORIGIN      
        1 aaaaaggtat taaaaagaaa tatttttaat ttgaattttt tcaataaaaa tttacaaaaa
       61 tgaaaaaata aacaaaaaag aattgaagat ttaagttgtt atttaaatat atatatatta
      121 agattttctg ctgaagatac taaaaaaata atatttttca taattttaaa aataataata
      181 aaaaaatgaa aatacaggct ataatctatt gagtaaacat agaaaagatg atattataag
      241 tatgtgctaa aatggttaaa gcaatattaa tattaaattt aactataaaa caaaaggggg
      301 cgttttttta tgtcaagtac agaaagcgta tctaaaaaaa gtggtcatcc gaagagtttt
      361 tggcttattt gcatgacaat tttgtgggaa agatttagtt attatggttt aacagcttta
      421 ttggtattat tttttacagc aagtgtagct caaggtggtt taggtctttc tactcaagaa
      481 gcaacatcta tgtttggctt atttacaggt cttatatatc ttacaccatt agtcggtggc
      541 tggttagcag ataaagtttt aggtcaacaa aaatgtattt ctataggtgc acttttaata
      601 gcaataggag atttcgtact atttgcttca tctacatcta ttgcaatggt ttgggtaggt
      661 ctatcaataa ttattttagg aaatggtttt tttaaatcaa gttgctcaaa tatagttggg
      721 gatatatatc ctaaaactga tgtgtcaaga aaagatgcag catacagttt attctatatg
      781 gcagtaaatg taggagcatt tatagctcca ataatatgtg gattgctttc tgataatgta
      841 tttgcagtaa gaggagcaag tggagatata gtatcttatg gatataagat ggcattttta
      901 atttgtggta taggcatgat gttaggtatg attatattta cacttcttgc accaaaatta
      961 ttaaatgaag tcggaaaata tcctgtgact aaaggtaata aaggtgaaaa aattgagtat
     1021 ggaccactta ctaagttgga gaaaaatagg ataatagcaa tggttatttt atttttattt
     1081 gtggtcttat tctggacagc atactatcaa acacaaagtt cttttatgat ttatgtaagg
     1141 gataatgtaa atagaactct atttggattt gaaatgccag tagcatggct tacatcacta
     1201 aatgctatat tatgtgtggc tttatgccca gtattagcta ccctatgggt taaattatct
     1261 cataccaaaa gaggtgattt ctctattcca gttaaaatgg gattaggaat gataataatg
     1321 ggtgttgggt ttatagttat ggtatttgct acattagcta atggtccaga tggaatggta
     1381 aaggctagta tattgtttat agtattgact tatatattta caacagtagg tgaattattc
     1441 ttatctccta taggacttgc aatgttcaat aaattagctc cagctaagta tgctacgttg
     1501 tctatgggag cctggtatct aacaagtttt tttgcaagtt tattatcagg aaagattgct
     1561 ggttttacag ccacacttgg ttttttacag atatttggag gaatagcaac agttttaata
     1621 atatttggtt taatattatt gggaataaga aatgtattaa caagattaat ggctatggat
     1681 ttactaacaa aagaaaaata attcatgacg aattattgta aataaatgaa ttactttaaa
     1741 taaaggaagt tacatttaaa tataaaatta aatgtaattt cctctttgat tatgaaaaaa
     1801 agctttacac aaatagatat tataattaaa attataattg tgataattag agtgtataag
     1861 atttatataa attatacaaa aaatatttat gtaataaact ttacatactt atatatttag
     1921 ttattcaaac tattatttcg aggaggtctt gttgtgaata taaaagatag attaagtgga
     1981 ttaagaaaat ttatggaaga aaaaaatata gatgcctata tgattccatc ttctgataat
     2041 caccaaagtg aatatgtagg agattacttc aaatcaagag aatttatatc agggtttaat
     2101 ggttcagcag ggacggttat tgtgactaaa gacgaagctg ggttatggac agatggaaga
     2161 tattttatac aggcagaatc tcaattagaa ggaagtacaa taaaattatt caaaatggga
     2221 caagaaggtt gcccaactac agatgaatat ttatataaaa atattcctga aggaggaact
     2281 ttaggatttg atggtagagt tatatctgct agagaaggag ctacattagc tgaaaaatta
     2341 tctaaaaaag gtataaagat agaatatcag tatgatttaa tagatggtat atggccagat
     2401 agacctgctt tatcagatag taaagcattt ttattagatg ttaaatattg tggagaaagt
     2461 tttagctcaa aattagctag attaagagaa aaaatgagtg aaaaaggtac ttctactcat
     2521 gtaataacta ctcttgatga tatagcatgg ttattcaata taagaggtgg agatgttaaa
     2581 tataatccag ttgtactttc ttatgcagta ataactttaa aagaagttta tctatttgtt
     2641 gatgaaagta agttaaatga agaaatacta aatgaactag ctaaggaaaa tgttcaaata
     2701 aagccatata atgatgtgta tgaatttgta aaaaatatag acaaaactga aaaagtttta
     2761 ttagatggaa ctaagcttag ttatacaatt tacaataata taccttgtga agttgagaaa
     2821 gttgatgagt tcaatccagt aatgtttttt aaagctcaaa aaaatgaggt agaattagaa
     2881 aatataagaa attcacatgt aaaagatggt gtagctttca ctaaatttat gtactggtta
     2941 aagaaaaatg taggtaagat ggaaatcact gaaataagtg ctactcaaaa attagaggac
     3001 ttaagaagag agcaagaagg tttctttgag ccaagtttca atacaattgc tgcatataaa
     3061 gagcatgctg caatgatgca ctatagtgca actccagagt caaattataa attagaggca
     3121 gaaggattat ttttagtaga ttcaggtgga caatattatg atggaactac tgatataact
     3181 aggactacag ttttaggacc tataagtgat gaattaaaac tacactttac ttctgtagca
     3241 agaggaatga taaatctttc taaggctaag ttcttgcatg gttgtagagg atataactta
     3301 gacatactat ctagaagttg catgtggaat atgggaattg attatcaatg tggaactgga
     3361 catggtatag gatttgtgct aaatgttcac gaagctccaa atggatttag atggagagta
     3421 gtacctgaaa gatttgatag tgcagttctt gaagaaggaa tggtaactac taatgaacca
     3481 ggtatatata tagaaggttc tcatggtata agaacagaga atgaaattgt agttagaaaa
     3541 gctgaaaaga atttctatgg tcaatttatg gaatttgaag ttgttacgtt agctcctatt
     3601 gacttagatg gtatagtacc agaattaatg aataaggatg aaaaagatta tttaaactgg
     3661 tatcataagt tagtttatga taaaatatca cctttcttaa ctgatgaaga aagagaatgg
     3721 ttaaaagttt acactagagc tatatagttt gtactagata caataaaaat cgaggtatta
     3781 taatgcctcg attttttatt gtatatttta ttaactttaa aaaattttta atttgataat
     3841 tctactaatt ttttattcat tataagtagt ataattccaa atatcaataa aaatgatggt
     3901 atcactatga agacttgtaa cataccaagt tggtctataa tagcttgcgt atagccagat
     3961 aatttatttg ctgcaaattt gcctaaaaac caaacaccca ttaggaatga aatatacttt
     4021 tcaggcgaaa gtttatttac cataccatac ccgactggag aaaagcaaat ttctcctata
     4081 ctttggaatg ttaaaaatcc aactacccaa aatacacttg ctttgtgaat tgaattaggg
     4141 tctgttcctg tttgtataac tgctactatc ataaacaaaa atgctatggc taatagtata
     4201 aaacctaagc tcattttttt tggcacactt aaatcgcctt ttttagtttg agataatttt
     4261 acccatacta aagcagagat aggtccaaga ataacacaaa ggagaccatt gtatgaatct
     4321 atccatgaag taggaatttg aaaattgcca acagtaagat ttatgtgttc ctttgtatag
     4381 agtgcaatag acatagaggc ttggttatat gctatccaaa aaaatactgt gaaaaaagct
     4441 aacaccaata ttactattat tctttttttc tcaatcaaag ttaaaggtct tgaagcgagc
     4501 attttcttat attctagtga ttttcttata gactctctcc cgttaatagg gtcattattc
     4561 aaaggtttat aaaactttct gtttccaaat ataaagaaga ataaacctat tagaaccgta
     4621 attgatgaaa ataaaaatac atatctatat ccatacatac ttatagataa tccgcttttc
     4681 atagcaaacc attcattagc aactaaaccg catattaaag ggccaaataa agtacctaga
     4741 ttagtagcca tatacgtaat agagaaagct ccatcttttc ttgggtcatc attggtatat
     4801 aagtttccaa ccatagtatg taagtttcct ttataaaaac ctattcctaa tccagataaa
     4861 aatatcatga tgtttatatc catcttacta tttgctatgt atcctagaaa ataaccaatt
     4921 gaaaccatta gcattccaag tataatacag tttttgggac ctaaaaatct atcactgacc
     4981 catccaccaa taactggaga gaaataagtc catgctaaaa aagatgcaag cattgatgcg
     5041 gattctgctg gtgttaaccc taaaccacct ttggagactt gttcagctaa aaacaataat
     5101 aataccaatt tcataccata gtatgcaagt cctacaaagg taaatattga acagcagaaa
     5161 tataaatctc taggttgttt aagtttttct tgcgttttta aaactgacat atgtcgagaa
     5221 cctccagata aatagtttaa caaaatttta ccatactata tgagtatatg tttttttcat
     5281 atagacgtca acttgtaaga taatttaatt aagtcgaaat atatgtaaaa atagtatcat
     5341 ttttgtgtat tcagtaatat ttttttatga taatatatgt tattattata ttaaaataca
     5401 aacatattta tggaaaggat atgccaattt agtagttaga ttgattgttt ggcaggtggg
     5461 atgagctatg ataagaaaga aatctgaatt acaagtagaa gtaaaagaaa atttaaatgg
     5521 tggtatagga aaggttaagc ttgaaaacat aatacaaaat gaagagctta aaggaaaagg
     5581 aagactattt aaaagagtag ctttaccagt aggaagttca attggtgtgc atgaccatac
     5641 aacagatttt gaagtatatt atatactaaa gggaaaaggt aaagtatttg ataatgggga
     5701 atttgtagaa gtaaatgagg gtgatgtagt gtatacagct gatggcgaaa aacattctat
     5761 agaaaatata ggagaagaag atcttgagtt tgtagcacta gttttatata tgtaaaatat
     5821 ggaggaggag tcatgacagg caagatagaa gaaattaagg acaagataaa tagattttgc
     5881 aactccaatt taaatcaaga atacaaagat ataagcttta aaattttgca aaacctttta
     5941 gataataata aagagataat tcattcttct agagctgata tatggtcttc agctatacta
     6001 aatatagttt tagaacaaaa tcttttatac aataagaagc atccactaca tataacaaaa
     6061 aaagagtttt caaaacaaat aggtgtttct ttaaatacta taaataataa atcgtcttta
     6121 ataaaagatg tttgtagtat tgacttttta aatcaagata ctatagctat ttctaatgta
     6181 gaattttggg taaaagagac taattccaaa tcaaagtctg tagatttaga tttagaaaag
     6241 aagaaaatac tatataaaag atatattaag ctatcacaag atgcgaatag tgatattgaa
     6301 tcaattagat atatggagga agctgtaaat ataggtaaaa gtatgattac taaagaagat
     6361 aaagaacttg gattttggaa aggtttatct accagagcct atatgatagc acttgaatcc
     6421 cttgctagaa agttagaaag tattaataat ctaaaggagt ccaaaaaagt attagaatat
     6481 ttaatagaaa ttaatcctag tgatgagcag ggaataagat acaaattatt aaatgtatta
     6541 attagattaa atgatagaac ttcaataaat aaattatttg aactttataa agaagaaaaa
     6601 tctgctacat ggatgtacag taaagctctt tattacttta aaaataaaag tatgtttttg
     6661 gcaaatgatt ctattaaggt tgctagaagt aaaaataagt acgttggatt atatttgtta
     6721 gattggcgaa atgcttttgg tagagaattt gttacagagg aagaaaaggc agaagctgtc
     6781 tattactatg atgaaaacat agtgatttgg aatgaagtta aaggttctat ggattggcta
     6841 ttaaaaaaaa tgttggaatt ttcataaaaa aaactactcc ttacttttat ataagtaaaa
     6901 ggagtagttt ttaatttata catctagttt taaatcttca ggttttatta gacctttttt
     6961 tctcataaat tctgaaatca acaaagttac aatagcagga agtataaaat taagtaaaag
     7021 tataccaacc cacatagata tacctccttt tccagcactt tgcatagcag ttactgttcc
     7081 aaattgtcca accaatccac aagtacccat accagaacca ataggaatat tctccatttt
     7141 gaatattgtt gtagataatg gtccaagtaa tattgaagct acagtaggtg gtatccaaat
     7201 tttccaattt tttattatat taggtacttg caacatagat gtcccaagac cttgtgccac
     7261 aaggccacca actccatttt ctctaaagct cataacagca aagcctacca tttggcaaca
     7321 acaacctaca gttgctgcac caccagcaag accacctaaa cttaacatca tacaaatagc
     7381 tgcactactt ataggtaatg taagagctat tccaacaagt gcagagacta aagctcccat
     7441 tattataggt tgcatagttg tagcttgcat tattatattt ccaaatgaag tcataaaagc
     7501 agaaacagct ggaccaataa gttcagctac aagtactcca attagtattg tcacagcagg
     7561 tgtaaccaat atatctattt ttgtttcttt tgaaactaat tttccaacct ctacagcaaa
     7621 aagtgtagcg acaaatgctc caacaggtcc tcctaattca ttaccacaag ctccaacaag
     7681 tgcacaagaa aatagtacaa gtggaggtgc ttgaagtcca tacgcaatag ccactgctat
     7741 agctggacca gtaacctgtt tagcaagagg gcttataact tcagtaaaaa gagaaatatt
     7801 gaaattatct cctaatgtat tgaaaattgt tccaattaaa agggacgaaa aaagaccgag
     7861 tgccatataa cttagtgcgt caataagata cctttgaaca gaaatttcaa tattttttct
     7921 tttagcaaat gatttgaaat ttcttttttt ataattgatt tccaatttaa aatcctcctc
     7981 atataatata agtataattt ctttttaatt aattatataa cttttcagct gacaagacaa
     8041 atgttatttc actaaaatta gactattgta atagtaagaa attaacatta attttagttt
     8101 taaattttaa atttatggtt tataattagt atagggatat tctgaaatat aataattttc
     8161 cagtttatcc caatatttaa cttaggtaga ttgcttacaa aacaaatgat tatatattag
     8221 gctaaaagga agtggataga tgatatttgt aaaaaataag aaatacagaa aaattgccat
     8281 aattatatct attcctatat tgcttgttgc gcagtactac ctttttaaat ttggaatact
     8341 tagaccaatg gtaaagggag tagaaattga aattgtagat ggggaatatg ttaaagatat
     8401 agataaatat gtagtaaaat taaatgaaac agttactttg tctacaggtg aatatattaa
     8461 ggttccttcg tatgctaaaa atccaaatat ttggtttaat gtattggata actcagggac
     8521 tttaaaaata gatggaaata aaatgacagc attaaaggtt gggtattctt ctgtgggtat
     8581 catgaaaaat tcaagagtat taaaaaaggc tactataaaa gtagtagacc cagaggtaga
     8641 atcactagat atggagatta atggggattt aaaacatgtt ggtgatagtg ctactataga
     8701 aagtacaata gaagttgatt atgataagtt taaaaaatca tataagccag tttataagag
     8761 tagcaatgaa aatattttaa aaatcaaagg aaataaagta agtgcagttg gagttggtaa
     8821 agctacaata tatgctaagt gtggaaataa agaaattgag agaacattta ggattcaagc
     8881 tagagttgct gaaataaata tggatgatat aaaaatagaa gttgaccaga atgaaaaatt
     8941 gaaaccagat ataataactt ctccacctgg tttagaacca ccaaaaatat attatgagtt
     9001 agtagattca aaattacctg tagaaagagc aataagttta agtagtaatg gaaatgttgt
     9061 agggcttaga gaaggtaaag atagggtgag aataacttgt ggagataaat ctaaagtaat
     9121 aacagtaaca gtagtcaaag aaactataac gaatgactat atacaaaact tggttggttc
     9181 gcataaaata gtaaacaata aagttattat taaacttgaa tgggattata tgaaagatgt
     9241 ctttgattat gatatatatc ttaaagataa gttgtcagga gaatcattta agaaaattaa
     9301 atctataact ataaatgaaa gtgatttagg taagtctaac aaggtatatg cgactataga
     9361 agtagatatg aagggtaatc aaaatataga ttttgatatt tatgtcatag gtaaaaagga
     9421 ttcagataca actaagccaa gtaatgtaat aaatattacc aatggaaatg gaaattcaaa
     9481 taatgataat aatattaaag atatgagggt agaaaatata tcagctaatg ttgatagaga
     9541 taataatgtc attagagtta actggagtag tatatctcat aaggactgta cttacagtat
     9601 atatgtaaaa aataatacta atggagatgg agaatttact ctatatcaaa gtgatataca
     9661 gggaaatgag catactataa gtattcctga aggagattta aatctagata tttatgttgt
     9721 tgcaaattat aatggaaata gctctcaaaa tagtgatgtt ataaatgtaa gatagatttg
     9781 tatacaaatt gaaagtgttg agtttattta aatattaaat taagaaaaag gatagtatag
     9841 ggtaattata ctatcctttt ttgttacaat atataggttt aaaatctcta gtaatataaa
     9901 ttataaaaag tcttatttac acaaatctat agatattttt tttattaatc aatattgtct
     9961 taaattgaac ttagtgcagc ttctattgca tgtattactg tcgtatcaaa tagtggaata
    10021 gaagtgtcat tttgtttaat taaaagacct atttctgtac atcctaatat aacagcctct
    10081 gcaccttgtt tgccaagctt atctataata gataagaaag ctttttttga agattcagat
    10141 ataattccta agcaaagttc attaaatatt gtgtcattta ctattatacg gtcttcttca
    10201 tttggaatta agacttcaat tccattatta ataattacat ttttataaaa atcttgttcc
    10261 attgtatatt tagtacctaa taaggccact ttttttattt ctttttccct taataccgta
    10321 gcagttacat ctgctatgtg aagtaatgga atatgtatac tttcttgtat tttatctgat
    10381 accttatgca tagtattagt acagataaca ataaaatctg cccctgcctc ttgtaattta
    10441 attgcagcat cagctaaaat tttggcgctt ttttcccaat ttccactaga ttggcattct
    10501 tcaatttctt gaaaatctac actgtaaagt atacatttag aagagtgtag tccacctaat
    10561 ctttctttta ttactgtatt aataacttga taatatgtaa tggtactttc ccaactcata
    10621 ccaccaatta agcctatagt cttcatatta tacctctctt atttatttat tttgaaatag
    10681 ctatttacaa atacaagtat agcatctaaa atagaagcat acaataataa aattgtatgg
    10741 agtacaatta taattaagag gagtaattat atgccaataa attcttttga aaactatcct
    10801 atgacatgga aaccaaagtt agataaccga aaaccaccaa tatataaaac tttagcaatg
    10861 ttattagaag aagatattaa aagaggaaat ttaaatcctg gagataaact gcctccacaa
    10921 cgggaattag cagattttct agatttaaac ttaagtacaa taacacgagc atttaaactt
    10981 tgcgaagaaa agggactaat atgtgcaaaa gtgggaaagg gaacattcat ttcttctgat
    11041 gtaaatgtaa gtaatacatt attatatcaa acggaatcaa aagacataat tgagttgggg
    11101 acagttcatc ctccgtatga gcaaaataca tatattattg actttattaa aaatgtacta
    11161 aaacaaccag aaatggatag gtttttacaa tacatgtctc catctggcac atatatgcaa
    11221 aaaaagtcaa ttgcaaaatg gatagaaaga aataatgtat acaccagtga agaaaatata
    11281 ttattatcta caggagggca aaatgctata tgtgcaactt tactaggatt atttaaagct
    11341 ggtgatagaa ttgctacaga ttctttaagt ttttctggaa ttaagtcaat agctaaaatg
    11401 ataggtattc aattagtccc aattcctcaa gaaaataatg aaatatcgat tgaatatctt
    11461 gaaagttatt gtaaaaatga aaatataaaa ggcatatatc ttataccaga ttatcataat
    11521 ccaacgacac atactatgtc tgatttatct cgtaaaaaga tagccaagat agcaaagcaa
    11581 tacaatataa taattattga agatgctata aatagcattt ttagagacgg tatacaaact
    11641 cccatatttt ctttagctag tgataatact atatatattt ttagtacatc taaattttta
    11701 tgtgctggtc ttagagtagc atttgtagtt gctccaaaac gatatataga aaatttagaa
    11761 aatgcactat ataatatgaa tctaatggtt tctccattta cagcagaaat agtacaccgt
    11821 ttattatatt ctcctattat agataaaatt atagaagaga aaagagatgc aattatagaa
    11881 agaaatgaag ttgctgataa agttttgtca gattataatt tgattggtga taagaattgc
    11941 agttttcgtt ggttacttct tcctgatgaa ttggatggaa aatcatttga aatttctgca
    12001 aaaaatctag gagttcaagt atattgtgct gaacgatttt cagtaggaaa ctctacagtt
    12061 ccaaaagcag ttcgtatttg tgtaacagct ccaaaagatg tagaagaatt agaaaaaggg
    12121 cttaatataa taaagtcact tcttaagagt taaatttatt ttactattat ttaattaaat
    12181 aaataaaaag gcggagaaat ttattataat aaacctccgc ttttattatt taatgaaata
    12241 ctttactttt tattaaaaaa tttaatttta ataatcttga aaatataatc gttttatggt
    12301 aaaataattc ttaaaaatag aaaaaattaa tcatttgtta aaaaaaatgc ataatttatt
    12361 gtaatttatt gacgaaggtt gaaaaatatt cataaatgtg cgaaataaca gaaaaaatgc
    12421 aaaaattaat tcaatttcat ttgttataaa atctatatgg caaaattaaa tctgcctatc
    12481 agaatgatat aaacacatca gaaaggagga cgctatcaaa taatacaagt agttaacttt
    12541 gatagcaaaa aattatgaat gaacaaacta gaatatcctt agagagagct gctgaattaa
    12601 aatcaaaaat tgatgattat attcaggcta gaaaaacgat taacagaggt cttgaaaaag
    12661 aagaagagat aaataaacga aaacagaaaa tattaagtat cttaaatgga actgaagagg
    12721 attggaataa ctacaaatgg caattatcaa atagaataac agatgtagat actttatcaa
    12781 aaattataac tctaactaaa aaagaaaaag aatatataaa agaggttggt actcaattta
    12841 gatgggcaat atctccatat tatttgagtc ttatagaccc agaagatata tgtgacccaa
    12901 taaaattact gtctatacca acacatatag agttggaaga tgagcaagaa gatttggacc
    12961 caatgggaga agagtataca aacccagcag gatgtataac tagaagatac ccggatagat
    13021 taataataaa tgtaacaaat gagtgtgcta tgtattgtag acactgtcag agaagaagaa
    13081 atattggaca acaagattct cataagtcaa aagctattat ccaagaatct atagactata
    13141 tcagagaaaa tgaagaaata agagatgtac tagtaactgg tggagatgct cttacattaa
    13201 aagatgatta tttagagtgg attcttagcc aacttaaaga gataccacat gttgattatg
    13261 ttagattagg tactaggact cttgttacaa tgccacaaag aattacagat gaattttgca
    13321 atatgctaaa aaaatatcac cctatatata taaatactca ttttaatcat ccaatggaaa
    13381 taactaagga atctaaagaa gcttgtgaaa agttagcaaa tgcaggcgtt ccattaggaa
    13441 atcaggcagt attattaaat ggaataaata atgataaatt tgtaatgaga tgtttaaatc
    13501 aagaattact gaaaataaga gtaaaacctt attatatatt ccaaagtaaa catgtaaagg
    13561 gaacaaaaca tttcaataca tcagtagatg atggtcttga aatcatggag tatttaagag
    13621 gatatacatc aggaatggct ataccaacat atatagtaaa tgctccaaaa ggaggaggaa
    13681 agactccttt gcttcctcaa taccttgtat caaaaggaac agattacgtt atgcttagaa
    13741 catgggaagg aaaagttata aaaatggaag atgaacctgc tgtagatata aagaaactta
    13801 taaaagaaca agcacaggat taaaaaaata tttaaagttt ataaaaagaa acaactttaa
    13861 aataaaaaga aaatggatgt caaatttata tcaatttgca tccatttttt attagttatt
    13921 tattattttg ttgttgatta actaattcaa gatatccact tgggtggaag taatatatct
    13981 cagaagtatt ttcatttaca aagtaaccaa tatcaccatc tgcatctggt aaaaatacat
    14041 aaccattaag atttttagct tttaatgctt caaaatcact agcatttcct tgatatatgt
    14101 aatcaacttc attgttgtat tttactaaaa gttctttagc ttgttcttga gtcaatttag
    14161 tattttggct tacaggaaca ttcttttgag aatctgcata agaagattca tttactggtg
    14221 cagttaataa caaacaagat aaaactaata aagatataag tgcttttttc atactaaaac
    14281 cctctttcat gtttaattat ataagattca ttacttaata atattgtatg tttcctataa
    14341 ttaaatatta ccattaaaga gaataatatt acagttacac agctgtaact tttgattaca
    14401 aaatcgtaat taaattgtat ttttattcca tttgtatttt atcatgtcta cgatttacaa
    14461 actcacagaa gtatatcttt ataacgccca tcacaggagc agatagaatc ataccaggta
    14521 taccaaagaa tcctccacca acagtaactg ctaggattgt aaagaatggg ctaagaccta
    14581 gttgtccacc tacgattttt ggctctataa atgtaacttc aacttgttga attattatta
    14641 agtaaattaa agaagttaaa gcaattgttg gattggagaa taaattaatt attacaactg
    14701 gtgccatacc tattactgga ccaaaatatg gaaccatatt catgacaccc ataagtgtac
    14761 caaataatag tgcatattca gattttaaaa agtaaagacc aattccagaa agtactccaa
    14821 ctataaaaga atctaaaatc ttacctgtaa agtatttacc aatatttaaa tttaagcttt
    14881 gacatagttc tattataaag tctccatatt ttttacctaa ggaaatataa actacttttt
    14941 ttgtaaacag taagaatttt tctttttcta ataatatata gaaacaaatt ataaaaccta
    15001 aaagaaattg tactataaat tttccaactg aaaatgtagt gctaaatatc ccaccaagtg
    15061 aaccaataag caagttacta aatttaggca ttacactcat aattttgtca ccatactctt
    15121 ttaatgtatt agggtcaact gattgtaaac tcttgcctaa gtcgaataaa aactgctctg
    15181 ttttatctat gtatgtagga atttgagcaa ctaaatcagc taaactattt actatggatg
    15241 gaactgtaaa taatataaac gaaattagta taactatgag tacaccataa gtaagaagta
    15301 acgaatatat tctcttaaaa tttaatttac tttctaagaa ctttactagt gggttgaata
    15361 tatatgctaa tacaaatgct attataaatg gtgttagtag tgacattaat aaacttatca
    15421 ctccaaaaaa atatttgtaa ttatcaataa ctttaatcaa gatatatgaa acaactacag
    15481 tgagtacaat atttagataa atttttttat cttgcaaaat aacacaacct ttcttatatt
    15541 tattaaattt agatatactt aaatctaagt taaagttaat atttatagct tattatacaa
    15601 taaaaagcag tattaatata gtatgattaa tatgaaatta taaatacgtt aaaactttat
    15661 gtttatagct aaaataata
//
LOCUS       DAEOSP010000082        15651 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.082, whole genome shotgun sequence.
ACCESSION   DAEOSP010000082 DAEOSP010000000
VERSION     DAEOSP010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15651
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.082"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(105..965)
                     /locus_tag="KRH54_002013"
     CDS             complement(105..965)
                     /locus_tag="KRH54_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBG8579670.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELIDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(1282..2610)
                     /locus_tag="KRH54_002014"
     CDS             complement(1282..2610)
                     /locus_tag="KRH54_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBG8579671.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2758..3087)
                     /locus_tag="KRH54_002015"
     CDS             complement(2758..3087)
                     /locus_tag="KRH54_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG8579672.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3111..4400)
                     /locus_tag="KRH54_002016"
     CDS             complement(3111..4400)
                     /locus_tag="KRH54_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8579673.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4416..4742)
                     /locus_tag="KRH54_002017"
     CDS             complement(4416..4742)
                     /locus_tag="KRH54_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8579674.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4877..5506)
                     /locus_tag="KRH54_002018"
     CDS             complement(4877..5506)
                     /locus_tag="KRH54_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579675.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5721..8378)
                     /locus_tag="KRH54_002019"
     CDS             complement(5721..8378)
                     /locus_tag="KRH54_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8579676.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKGIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(8737..9681)
                     /locus_tag="KRH54_002020"
     CDS             complement(8737..9681)
                     /locus_tag="KRH54_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8579677.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(9674..10699)
                     /locus_tag="KRH54_002021"
     CDS             complement(9674..10699)
                     /locus_tag="KRH54_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8579678.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10774..12342)
                     /locus_tag="KRH54_002022"
     CDS             complement(10774..12342)
                     /locus_tag="KRH54_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG8579679.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(12455..13366)
                     /locus_tag="KRH54_002023"
     CDS             complement(12455..13366)
                     /locus_tag="KRH54_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8579680.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13350..14336)
                     /locus_tag="KRH54_002024"
     CDS             complement(13350..14336)
                     /locus_tag="KRH54_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8579681.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14696..15097
                     /locus_tag="KRH54_002025"
     CDS             14696..15097
                     /locus_tag="KRH54_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG8579682.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            15100..15498
                     /locus_tag="KRH54_002026"
     CDS             15100..15498
                     /locus_tag="KRH54_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBG8579683.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
BASE COUNT         4935 a         2727 c         1732 g         6257 t
ORIGIN      
        1 aaatacaaat tatattttat taatttttac tacttgtaaa ttattatctt atacaatgaa
       61 tttgtattat ataataagta tttttaggat tctgttacat aatattattt aaatattctt
      121 ctatatttct cttcttcttc ttcagtaaac acattaaata caacttttaa atttttttca
      181 ggattttctt ttagccatag tctaactcta cttacagcaa gattagctgc ctcttttttt
      241 ggataaccaa atacacctgt agaaattcca caaaaaacaa tattttttat ttcatcaatt
      301 tcttttattg tatttaaaca tgatttataa caatgaagca gttgcttttc ttgctcttta
      361 cttggctgac caccagatac aataggtcca acagtatgta ctacaaattt tgcaggtaaa
      421 caataacctc tagttatctt agcatctcca gtatattcta aatgcccttg tttttttatt
      481 attttgtcac aatcttctct aagtctagga cctgcgcatg aatgtatctc attatccacg
      541 catagatgta atggttgtaa acaccctaat aacttgttat ttgcagcatt tactattgca
      601 tctgctctta aattcgttat attacctctc catatggcaa tcccttcctc tacttcttct
      661 atatcattta catctattaa ctctctttct tcacattcaa tcaacaataa attatgtaac
      721 atattaagta tctctggagc aatttctttt ggtggatata cattcaaaag tcctctaagt
      781 atatctctag gcttaatttc atcatttgat aaattgtata ttttagaagg aacttctttt
      841 gaaaaatatg caattaaagt gtttatattt tttcttcttt gttcgtcagt tagaggcttg
      901 atagttttat caaaatcctc aaataaattt acatcatcag cataatctct ccacttcata
      961 gccatctctc cattcttgta ttttagactt aaaaataatt atgtatttat gtacatattt
     1021 atttatttct ataaacacct acataaatcc tgttaaaagt gtaaattttt tattatgcaa
     1081 cttctctggt tatttctatt gattgatagt aaattattgt atctaaattt aatccaacta
     1141 acaagtaact aaacctactt tcctaactgc aaaatacact atgttttcca cttgtatctc
     1201 atttgtagat aaataaagtc cctacaaata cattaactaa gctatgtact aaactgtagg
     1261 aactttaaaa atatattatt cttaattaag ttttggccaa taatttttat ttgcttctat
     1321 taaatcatct agtaattctt ttgcaactgt tgcactagga actgtcttag atagcataag
     1381 tgcttgccac aatttattat aagacttctc aatccaagca tcaactacta atttttcaac
     1441 tgctaattgt tgttctatta aacctttttg gaatgttggt attttcccta ttgataatgg
     1501 ttcatacccg tcttttccaa ctatacatgg aatttcaacc atagcatcat cttgtatatt
     1561 ttctatagct ccattatttt gaactatcaa taacatacgc tcctgtgtat taaaagctag
     1621 agctgtagct aaatccacta taaactctgc atgtattcct atcttaagcc cactgccctt
     1681 agaatgtcct tgcttttcta tcaatgcaca tgaaccaaaa acttcttttt ctcttccatc
     1741 aataacttca ttggcacgag tataatttac atcagaatga tttacaacat aatctggaaa
     1801 tagatagtac tttaagtaac tacttggtat tgtagttaaa tctgttttta ctatttcttt
     1861 agtaaataaa ttagtttcaa accaactatc tttcttgtca tttttcattc cttcttcacc
     1921 atttataaaa ccatattctt ttatatgttc tactaatttt ggtaaaagtt catttccttt
     1981 tttatcttta attgaagtat accaaccaaa gtgatttaat ccaaagtatc ttatatccat
     2041 atgtttacga gatttgagtc ctgcaatcct tgctatatta tgttccatac ctattggcat
     2101 atcacaaata tttattaccc tagaattagg tctcaatctt ctaacagctt ccgctattat
     2161 tgatgctgga ttagaataat ttaacatcca acaatttgga gaatactttt ccatataatc
     2221 tatgttttca agaacacctt ctatagacct cattccataa gctattccac ctggtccaca
     2281 agtttcttga ccaactactc catgttttag aggtatcttt tcatctagtt ctctcatttc
     2341 aagttttcca actcttatat gagctaaaca aaaatctaca tctgtataag cctctttagg
     2401 gcatgttgtt gctaaatact ctatattagg gtccttttcc tttattagta tttcacaagc
     2461 tttagctacc ttattttgtc tttcttcatc attatcatat aatttgattg ctcttaatgg
     2521 taacctatct aagttatcta gcaacattaa tatgatttct ggggtatatg tacttcctcc
     2581 tccagctatt actactgaaa atttttccat aataatatct ccttttctat ttaaaaattt
     2641 actgtattta taaataaatt tataaatact agtactttaa agtaaaaggg agttcattta
     2701 ttaaatctat tgatttaata aactatagcc aaacttcaag cactatttac aagaaattta
     2761 attcttatta attcttgggt ccatcatctc aaatatatct acaagttcag atactaaaga
     2821 atgagcaaat aatgcatttg aataatggtc ttcagagtgc acaactaaaa aatcagtccc
     2881 actatcttca ctattcatag catttgatag taattctaaa tgatgctttg atgctgtttg
     2941 tattttctct gaggctgaat ttaaaaggtg ttttgcttta ttgtagtcac ctttttttat
     3001 ttccttaaga gacatcatta catcactttt agcatctcct gcttctgata ttatactaaa
     3061 tactttttct tccatttcag gtaccatatc cttctccttc taatgacttg ttaaaaatca
     3121 aagctatcat catctaatgt tgtagctaat tctgcatttt tatttaattc ttccttctcc
     3181 attatcttaa agaatggata atatatcaag gtcataagta ttatacttgc aaatactaat
     3241 acgattgcct tccaatctaa tgtcatcaaa aatgctgcaa cccctggagg taaaaagcct
     3301 ggtggttctg caataggtaa tcctactaat cctactttta atgccataaa attaagtgct
     3361 ccaaccaata caggtccaaa tacaaatggt acaaacatat atgggttcat aactattggt
     3421 gttccaaata ttactggctc atttattcca aatagtgatg gtaaaatcac aacttttccc
     3481 atactattaa aactcttaca cttacttctt gacataaggt acactaatcc caaagttatc
     3541 cctgaaccag ttacttgcaa gaaaccatag taaaatccta ttgtaaacac atattttggg
     3601 tctcctcctg ctgctatcag tgttgcattt tctgccatat atgtagctgc tattggcatc
     3661 catatacttg aagttattgc tgctccatga agcccaaaga accataataa ttgagttata
     3721 aatagtatta tagttactgc aaaaacatta tctacactgc ttatagctgg tgctaagaaa
     3781 ttcattataa tagctggtag tggttgacct gagacatttt tagttattgc tgctattgct
     3841 gtaaataaca ttatacatac tatcattgga gttaagtttc taaaactttt atttactaca
     3901 ctaggtactt cttttggaaa tctaattacg agatttttat cattcataaa cttagtaata
     3961 tcaactacta aaagtgctac taatatactt acaaataaac cttgtgcatc taaataactt
     4021 gtatctaata caccatctac tacttgtcca catacaacaa gttgtgcaac aactgctgtc
     4081 atacctattt ctacatgatt aagattatat tttttagcta gtgcctgagc tataaaaaat
     4141 gctgaatata tagataataa actcatagta aatttattag ggataagtaa tgtatccata
     4201 ttttcagata ataatgtttt tgcacttcct tcaggtaaca tattgtttaa tgccataaat
     4261 aacatactta aagaaccaac aattattatt ggggtaatgc ttatcatacc ttcttttata
     4321 gcttgaagat gcctttgttt ttcaatcttt tgtgctattg gaagaagaaa tcttcttaaa
     4381 ttattttgaa gtgcttccat ttttattccc cccaattatc tatttttata taattcgatt
     4441 gccgacttca taacagaagg agcatccata cgaccataat gcttagtatc tattaaacca
     4501 actgctattt tgtaattctt acaagcttca actacatcat cttccttaaa cctaatttgt
     4561 ggtgctacaa gtactacatc tgtatcatta gcttcaatgt atgattttaa attttcaaat
     4621 gatgttgcct ctatatttat agttttagtt tttaactttt catgctcact acaaaaactt
     4681 ttcatctttt ccacaagtac acctgttgaa gctccatttg cacatactaa taaaatattc
     4741 ataattaccc tcctacaata tctaacctct tttgttttct aattttttat cagccaattc
     4801 ttactctaat tcctatcagt ttttacttta attcttatta ttttatttta attcttatta
     4861 atatttactc taattcttag tactgccaat gattaaactt gaaagccgta tctataactt
     4921 cttcttgctt atatttttct aatattttta agtatctttc tttataatca ttatctactt
     4981 ctttttctgg tattactata ctagcttcat ttaaaaagtg gaatgaatcc cttcccattt
     5041 ctaatcctct atatgtatgc ttgtcaagtg ccacgtccat aacttctgga actttaccca
     5101 ttgcaaatcc ttttataatt atatttttta ataaatcact tgatctatct ttctcacatg
     5161 cacaaagata tcttattgca tgtatgaaaa ataaaggtct gtctccatct gcatatgaat
     5221 attcctttct tacttgattt aaattattta tgattattac agctgatggg tcccccattc
     5281 ctatatcttc aactgatatt gtaagtatcc ttctccatag cttatcttct aattctggtg
     5341 atgatatata tagttcataa gcaaattcac aagcttcttc caccatattt cttcttatag
     5401 ctttttgaaa tgacgatatt atttcgtctg cactaaatcc gtttcttgta gtcattcttg
     5461 cccatgggtc atttgccttt ttatctctta atattttatc ttgcatatta tttactcctt
     5521 tttatttaaa ttttaattat ttatattgct catttaataa tataagcaag tattatgcca
     5581 gtttttttag acactttaaa acactgttat ttcaatcatt tttatcatca aaacttagat
     5641 actttgtcta agtatattta tatgtgtcta atattaatat tattttagac taaaaaaaat
     5701 taccaaataa gtttttatac tcattggtaa tctttttcga ttatttctgc atattctaat
     5761 attaataata tttgatagca ctctttttca ttaatttcta tattatattt tttatctaat
     5821 tgagatacta tatcttttat tttgcttaga ctcttgtgat aatcaagtgt tttttcttta
     5881 tcaagctttt caatttctct agtatttcca atcaacccat ctaccatgaa agctaaatgc
     5941 atcataagtc caattacttt atcgtatttc ttttctatat taaatactaa aaacaattca
     6001 tcagacacat attttatacc tttaaatatc ctattataat tagagctttt taaggatgtc
     6061 tttaaacctt caactacatc atcataacct atacttatct ttataatatt ttttaattta
     6121 ttgatatttt catccttatc tatatctttt ttgctaatat agtcaattcc tctaatatta
     6181 attctttgtt cattgattac tgcaacaata ttactacttt ctttaagttt cgtaatattt
     6241 aaacttaaat ctttttctgt tttatatggg acagatataa tatctattcc atctaagtac
     6301 ctactcatct tttcttctaa tatttttctt gttttattat ctacaccttc tgaaacatgt
     6361 gatgctataa taattgtttt agacagattt tctttatttt gaatttcatt agttccaaaa
     6421 tagtactttc tcgtgttagt tatagagttg tatacgtcat ttaaaggtaa tcccatattg
     6481 gccttctgaa ctgcttctaa cacagttaat gtcgtcacca taggaattac tcttactcca
     6541 cacttttttt ctttctttat aatttcatca aaagagttta atgaacccat atctgtaaat
     6601 agtaatacat ctttattgtc tatattatta attaaatttt taaaatctac aattacttca
     6661 tcatagcttt tatctaactt catatcgaaa gaaatagcac tgcactttcc aataatatca
     6721 ccaactgctt ttaccatact acttgctgta gtttctccat gcatagcaac tataatatta
     6781 atgttattca ctattttatt ttgttcatct tgtttagctt gtactatgaa taatgtaata
     6841 tatgctacag tttctataga taaaaataaa tcaaattcat tttcaattat tccaactatt
     6901 tttaatgctg ttttaaattc acttctgtta tttcttctaa tttgattcaa atctatgttg
     6961 tttgtagagc tacttttatc caatacgctt tttatataaa ttgccattga cttaaatatt
     7021 gattcactta tgtctatttc ttcatcttct ttaataattt gtcttattga ttcacacatg
     7081 cttttaaact taacattatt aaatagagaa gcatctgcta tattcatttc tacagattta
     7141 ttaagattag tatttactgc attaataata tttatatcat tgtagttaat aacttcttct
     7201 tctcctaatg acgtaaatac aaaatattct attccatgtg ttataacttc atctgcttta
     7261 atagacttct ctctataatc atcactcact tcatttatag atttatcaaa atattttttc
     7321 tctatataaa ctggttcttt attatctctt ttatatctta gatatccatt tgcaacagct
     7381 aattttatat cattttgaag ttgacctaca ttatttttac aatcataaag taatagtgat
     7441 ttaattattt ctttatgtac ttttattgaa atagaaatac tttttgattc ttccttgaaa
     7501 aaatttttga ctaattccat tctttcaatc attgtcctat ctctcaatga tggtatttct
     7561 attttcattg gtattcttct attgaatgta tctaatagtg ttgatgtcgg attttctgta
     7621 gtagcacata ttattaatac cttagactct ctttctttcg ttgcatctcc tagtctttta
     7681 aatgttccct tatctataaa agtaaacaac atttcttgac cttctgctgg tagtctatga
     7741 atttcatcta aaaatagtat ccctccattt gcaatttcca aaagaccttt tctggaagat
     7801 tctgcacctg tataagctcc ttgttctacc ccaaaaatat gtcccataag taattgtgca
     7861 ttattagcat aatcagcaca attaaatgtt acaaaaggag ctttattttt tctaattcca
     7921 ctatctatag aataatcgta catatatcta gcaatcatac tctttcctac tcctgtttct
     7981 ccatagataa tagtatgaag tccatttgta ggatagagca ttgcgacctt gcatttttct
     8041 attataggtc ttaaactatg ttctttacct attagtgttt caaaactatc tttttttatt
     8101 ttgttttcta cttttgttcc tttttttatg ccaagcaact cactttgtgt atcaagcaat
     8161 tcattcttgt taaggttaag atttgcaggg atataataca caggtcttcc ttttatcttc
     8221 gtaaccatac cctcattgaa taattggttt aaatatgtac ttatattgtt tcttgcaatt
     8281 cctgtctttt ctgataattg gttagctgta atcccttttt cattttccct atatgatgat
     8341 tctaaaactt tcattacttt atctttttta tatatcatta caagctcccc tacattatag
     8401 tattttatta actaaaaccc ctatacacac tttatttttt aataaactct tattctttaa
     8461 ctaaggttat aatttatcaa attttcctta ctttaaatta tagaaaatta ttgtagaaaa
     8521 aataagatta ttgctcattt tttctacatt actattttta attttatagt actttcttaa
     8581 caacatactt tacttgtttt taattttatt atatttgaat ttattatatt tgaaaagtag
     8641 aaataaaaaa ccctattttg gagtagtttt aaaatttctc caaaaatagg gttttagttt
     8701 gtatttcaaa taaatacctg ttcaagtatt tttatactaa acaactttta aattttgttg
     8761 atatttattt atttctgatt tagaagaata tacaaaatgt ccatctgaaa cctcaatcca
     8821 acttaatgac ttttcatttg aacaatcata tccattagaa ttatactcta ttctatttct
     8881 tgacttagca tatctagggt caggaacagg aattgcagaa agtagtgatt ttgtatatgg
     8941 atgaatagga ttattataca attcatttga atctgccaat tctactaatt ttccattata
     9001 cataactcct atacggtcac taatatgtct caccatagat aaatcatgag ctataaataa
     9061 acaagttaaa ttcttttctt gttgcaattt tttaaataaa tttactattt gtgcttgtat
     9121 agacacatcc aaagaagcta taggctcatc agctattata aattctgggt ctactgaaag
     9181 agctcgtgct atgccaatac gctgtctttg tccacctgaa aaatcagatg gatacttatt
     9241 tgcataactt ctatcaagtc ctactagctt tagcatctca tatactttat tcattctatc
     9301 tgttttattt ttacagatac cttgaatttt tagtggctct gaaattatat ctgcaattgt
     9361 catacgtgga tttaatgatg aagtagaatc ttgaaaaata atctgcatac ttttgtttac
     9421 atcttttttt ataaatttgt acgttttttt atctgatatg caatttcctt tgtaaattac
     9481 ttctccacca gtagattcat gtagtcttat tatagtttta ccagttgtag attttcctga
     9541 acccgactct cctaccaatc caaatatctc acccttatat atatcaaaac ttatatcatc
     9601 aactgctttt acagtagtac ttttgtccaa atgaaaatat tgctttaagt tttttatttc
     9661 cagtattttt tctctattca ttagaaatca cccttccaca atttactcta actggtacat
     9721 ctacttcagg tgcatctggg tgcaataacc aagttgctgc actatgtgta tcattaattt
     9781 taaacattgg tggctctttt tcataatcta tcttaagtgc atttttattc cttattgcaa
     9841 aagcatctcc ttttggtggg ttcaataaat ttggaggcat tccaggtata ttatataaat
     9901 agtcatcctg cgaatctaaa gtaggtaatg aacccaacaa cccccaagta tatggatgtc
     9961 tagggtcata aaatatatcc tctacagttc ctatttctac aatctttcct gcatacataa
    10021 ctgctattct atcagctata gttgcaacta ctcctaaatc atgagttata aatattattg
    10081 acaaacctat tttatgttgt aaatccttta ttaaatcaat tatttgagct tgtatagtta
    10141 catctaaagc agttgtaggt tcatcagcaa tcaatacatc tggattacag gcaagtgcta
    10201 tagctattac tatacgttgt ctcattccac ctgaaaaatg atgtggaaat tgtttaaatc
    10261 ttttctcagg attatcaatt ccaaccaatt caataagctc tatggctctc ttttttgctt
    10321 cacttttact tataccttga tgaatgatta cagcttctgc aatttgcttt cctattgata
    10381 tagttggatt gagagatgtc atagggtctt gaaatatcat tgatatattc tttcctctta
    10441 tatcttccat ctctttttca gacttcttaa ccaaatcact tgatttaagt aaaacctctc
    10501 catttttaat atatccattg tatgggagta tcctcatcaa actcttacac agtacagatt
    10561 tcccacaacc agattctcca actatagcca atgtctcacc tttttttaga ttaaaagata
    10621 catttcttac agcctgaact tctccttctt ctactttaaa acttacagat agattattaa
    10681 cctccaataa atgttccaaa aaccatcaac ccctatgcta ttcaaacctt agggatatat
    10741 ttttatccct aaggtttcta attaatttca gtattattca attgtccagt cagctatgtt
    10801 ccagaatata cctacaccat gatgacctaa aactgtatca ggtgttaatc cttttatgtt
    10861 tggttttcca acatatattg catctatata agcaataaat gtataaggca tatccttagt
    10921 catttctact tggaattgtt tatataactt taatttttca tctttatctt ctgtttctct
    10981 tgctttttga agtattttat ctatagtagg attagagtaa gcactgtaat ttgcaccttt
    11041 atctgttcca aatactttat atgtatggtc atctgggtca aatggacttc cccaacctat
    11101 taaatgagca tcttgattag cccaatctgt ttcagttaca acaacagctt tagcatcaac
    11161 accaatttct tttaactgtt gtgcacatat tttagccata tcaactctaa cttggtcact
    11221 ctcacctgct gttatctcga aagctaactt agtaccttct ttttcataga ttccatcaga
    11281 acctaatttc caacctagct tttcaatttc ttgtttagct ttttctggat tatattcaaa
    11341 cttttctata tcaggattat tatatggtcc catttgtaaa ggtgaatatg ctggtacacc
    11401 atgtcctaat aatacactgt caactattgc ttttctatct attgcatagc ttaaagcatt
    11461 tggtaatcct ttagcttttt tatctttaaa gaatttagaa ttaaaattgt ataatatacc
    11521 tctatagtcg gctgttttca ttatatttac tttaaaattc ttttcgtctt tttcaaagtt
    11581 agacatatct ttaggagtta tttgagctag gtctaattct cctgatttta attgcattgc
    11641 cttagcctta tcatctggaa caatcttaaa gactacttta tctaatcctg gttcttttac
    11701 aaaataatca ctattcttaa caagtgttat actttgacct ttgtcccatt tttcaagttt
    11761 aaatggtcca gtacctattg gtttttgatt aaattcatca gtagctatat ccttaccttc
    11821 caaagcatgt tttggtaata ctcctactgt taaataatca agcattgctg tatttggagc
    11881 ttttaaagta atttttatag tattatcatt aaccacatca atttttgtta tatcttcata
    11941 atttgatgct atctcagaag cattatctgg attcattata gtttctattg taaacttaac
    12001 atcatttgct gtaaatttct ctccatcatg ccacttaaca tcatctctta aattaaaagt
    12061 atatgtattt gttgcctcat caaatttcca gtcctttgct agacaaggaa ccaccttatt
    12121 attttcatca tgagctgtta atccattaaa tattaacgaa tttatttctc catgttcgta
    12181 tagtgcagga tttatacttg tatagtcatt acttccataa acaagcacct ttccatcttt
    12241 aggcgtatct gccttcttat ctttgtctcc tccactagaa caacctgcca ttagactaag
    12301 tatcattact aaacttaaaa cttttaactt ctttaatttc atgatgctcc cccttttgct
    12361 ttttataaaa tatttttcaa agctttgacc ccatttattt aaatagatat ttcaaatacc
    12421 tcagtatttg cttatttaat actcaaatgc tgatttataa attactagat tttttattat
    12481 tattctttct tatataatta cctatatttg ttatacacac caatgtggta accaaaaata
    12541 tacccggaat cagtataatc caccatctat ttgaaagtaa tgcttcctct gacaaagata
    12601 acatacttcc ccaagaaaca atttctattg gtaaaccaat tcctaaaaag ctaagtgtcg
    12661 attcagtacc aatagctgcg cctatgtttg ttacaaccat gaacattatt gatgccacaa
    12721 aatttggaag taaatgttgc tttaatacat agaagaatcc tcctcccatg ctcttagctg
    12781 ctagtatgta ttcgctattt tttatttgtc taacttctgt tctcactatc ttagatatat
    12841 tcatccaact tgttatccct attactatgg acatagatac tggatttgaa tttccaagta
    12901 tagcttgtac aaatattata attaatattg aaggtatact aagtatgatt tctgtaaacc
    12961 tcatcattgc atcatctaca agttcagatg ctgaaccact tatactacca taaacaattc
    13021 ctataacagt tgatattaca gtggaaaata gtcctataaa taaagatatt tttcctccat
    13081 accatatcat agaatatata tctctaccca ttgagtctgt tccaaataaa aaagttttat
    13141 ttggagctaa attagaactt gctaaatcca tgtatgttgg ttcatgtgtc attataaaag
    13201 atgagaatac acttccaatg actatgattg ataaaataat aattgatata tatggtaatt
    13261 ctttaatttt tttagataag gttttctttg tttctatatg ttcatcttct gctactaaaa
    13321 tataattttc tcctacaatt tcaaaatcac tatattgtgg cttcattagt atcacctcta
    13381 tcatatttca ttctagggtc tattttattg tttataattt gagctaacat attcccaaat
    13441 actactaaag cccctgttat taagcataaa actaataaca tattataatc atgatacttt
    13501 gcactttcaa agcaaagtgt tcctaaccca ggataagaaa aaactttttc aactacataa
    13561 gttcctccta gtatatgagg tatcgaaatg gccataatac taatatatga tggcattata
    13621 tttctcaggc aatgcttaaa aacaatagtt ctattattta aaccttttgc tttacataaa
    13681 agcacataat cttctcttat ttcctctagt aatttatttc ttatcatata tgtgtaatac
    13741 catagatggc ttaatataag tactgtaaga ggtaagatta gatgtgatat cctacttaat
    13801 atactggact cttctcccat tgaataagca ccactacttg gaagtatact taagtttata
    13861 ctaaatataa gtatgagtac taaggcaacc caaaatgatg gaatacagtt ggttattgtt
    13921 cctaatttgc atatgattct atcaatgagc ttatcttcat gtaatgtaca aaatattcct
    13981 aacaataagg ctagcacaaa ggtaagtata tatcctgaac cacctaatat tatagtgttt
    14041 atatagacat catttataac agaagtaaca ttttgtttat atttaaatga aattccaaat
    14101 tccccttttg atgcatttga aatccatttt atatattgac tgtatattgg ttcatccaaa
    14161 ccaagttttt tcatagcatt ttctttttct tgtgtactca ttctttctac tccatcccca
    14221 tagtaagaga ttaatgggtc tcctggagca agtcttgcca tgtaaaaaac aataaaagat
    14281 aataaaaaca ttactaaaat aaactgcaac aactttttca gtatatcctt taacaaaccc
    14341 caacccccct ttgttgcaat aaaaaaaacc acataaaagc aaacgtaaag taacgttaaa
    14401 accttcgtgg ttccattatc attaaacaaa attaaatttt ctatatttat ctatgagtta
    14461 attttagcat catttattaa ctgtgtaaac aaacttttta aaaattgtta aaatttcatc
    14521 aaattatttt atttatacct aaaataaaat ttttatattc tattcttcat tattttctat
    14581 aaaattataa aattttaggt tgactatata agaaaagtat attatattag tttgtatgca
    14641 aaccatttaa gaggtgattt tattgaaaag taattatgac tgttttagaa tagccatgct
    14701 cttaaaagaa ctttattcta aaactatgta cacagtagaa gaaaatttta aagaaaatgg
    14761 attaacacac caacagatta tagttataaa actgatagct cataatcaag aacttacaat
    14821 atcacaacta tgtgatgaaa tgtctttagc taaaggtacg gtttcaggca taatcagcag
    14881 attagaacag attggatata tagaaaaatt caagaaatct aatgataaaa gaaatacata
    14941 cgttaagttt acaacaacag gatttgaatt tgccacaaat tttaaaatta aaatgcaaga
    15001 aagttttgat gatattttta aaaattgtga tgaaaacgaa ttgagtgact tagtaaaaaa
    15061 tcttagaaat atattggcaa aagtaaagga gagataatta tgaattataa attaatactt
    15121 gcaatagtat ttataacatc agctttaatt ttctacacta taggtgtatt tggagaaaga
    15181 aaggcaaaaa ttttaaaaaa gaaacatgta attatatttt ggcttggttt tatatttgat
    15241 actttaggaa catttacaat gagtaacata gccaacagcc atacttttga agtaaaatct
    15301 gctttatcac aaaaccttca tagtattaca ggtcttcttg caattgtgct tatgcttttt
    15361 catgcaagtt gggctacttt tgtcctatac aaagatgatg aggaaaagaa aaaattcttt
    15421 cataagttta gtatagttgt atggactata tggttagttc catattttat aggtatgttt
    15481 attggtatgg ctggatagta aaataaaaag tgtgaaagaa ggatgtaggt aaatttgtat
    15541 aagacaaatt tacctacatc cttctttcac acttttacat atttatttat aatactccat
    15601 tatttctttc atgttcctta atacctatat agaatattta attgtttcta t
//
LOCUS       DAEOSP010000083        15639 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.083, whole genome shotgun sequence.
ACCESSION   DAEOSP010000083 DAEOSP010000000
VERSION     DAEOSP010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.083"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            73..711
                     /locus_tag="KRH54_002027"
     CDS             73..711
                     /locus_tag="KRH54_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579684.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(1189..2547)
                     /gene="rlmD"
                     /locus_tag="KRH54_002028"
     CDS             complement(1189..2547)
                     /gene="rlmD"
                     /locus_tag="KRH54_002028"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBG8579685.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVAKII
                     KQ"
     gene            complement(2722..4482)
                     /gene="pyk"
                     /locus_tag="KRH54_002029"
     CDS             complement(2722..4482)
                     /gene="pyk"
                     /locus_tag="KRH54_002029"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBG8579686.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(4534..5493)
                     /gene="pfkA"
                     /locus_tag="KRH54_002030"
     CDS             complement(4534..5493)
                     /gene="pfkA"
                     /locus_tag="KRH54_002030"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBG8579687.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(5702..9271)
                     /locus_tag="KRH54_002031"
     CDS             complement(5702..9271)
                     /locus_tag="KRH54_002031"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBG8579688.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(9428..10375)
                     /gene="whiA"
                     /locus_tag="KRH54_002032"
     CDS             complement(9428..10375)
                     /gene="whiA"
                     /locus_tag="KRH54_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBG8579689.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(10392..10817)
                     /locus_tag="KRH54_002033"
     CDS             complement(10392..10817)
                     /locus_tag="KRH54_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBG8579690.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(10886..11992)
                     /locus_tag="KRH54_002034"
     CDS             complement(10886..11992)
                     /locus_tag="KRH54_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBG8579691.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(12004..12861)
                     /gene="rapZ"
                     /locus_tag="KRH54_002035"
     CDS             complement(12004..12861)
                     /gene="rapZ"
                     /locus_tag="KRH54_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBG8579692.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(12892..13605)
                     /locus_tag="KRH54_002036"
     CDS             complement(12892..13605)
                     /locus_tag="KRH54_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBG8579693.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(13609..14523)
                     /gene="murB"
                     /locus_tag="KRH54_002037"
     CDS             complement(13609..14523)
                     /gene="murB"
                     /locus_tag="KRH54_002037"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBG8579694.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(14796..15566)
                     /locus_tag="KRH54_002038"
     CDS             complement(14796..15566)
                     /locus_tag="KRH54_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG8579695.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT         4806 a         2987 c         1711 g         6135 t
ORIGIN      
        1 aaagaggaga atgtatataa gagtattcaa tgtactagaa aaaatcaaaa gaaatattag
       61 aagaaatgga tattgagtaa tcaaagaaaa gattttgata ttacaaatga tatgtttcaa
      121 gaaagcattc ctatcacaac aaaaattatt cttgaagaca tgccttcaaa tgatgaatta
      181 aatcatgttt tttcaaaagg ttgtgaacga aaaatgaaaa agagaaaaat tgtattaatc
      241 acactactat taataggtgt tttgttatta ggaagtatcc tatataatct ttttttagga
      301 aaaacagcta atatttcaat gctaaaggaa tcttggaatt ttgatatacc aatacctaac
      361 aaagaaattg aagtatttga tactcaagat agtattaatg gagatggaca aagttatttt
      421 attcaaggat tttctgaaaa aaatttcaaa aaagttttta atttaaaagg tggtatagtt
      481 gtaagcaaag ataatattaa tgaaatagaa aaatatattg ataaatttaa aagagattct
      541 gttaacatta ataaaagtaa taaaaataaa attgaagaag attttaaaaa atataagtta
      601 gaagttaaaa aagatgataa gtatatatat aagagaaatt atgagaacta cgtagtttta
      661 attataaaaa aagatgaaca aaaattatac agtttaatat ggaatcagta ggtacatatg
      721 tattattaag aattataaat aataaaaata aattttagtc aatatacaaa aaccttttaa
      781 attgtctgga aatcacctct tcatttgtta taaaaacata caacaaatga agaggtgatt
      841 ttatggtttt aaaaaaaata attataagtg gatatgtgct ttcacatgtt tatcaacatt
      901 tgtaatgcct atatttgcta atgaattata atataaaaat attaaataat attcaactat
      961 agattatgta aattactcta tgaaaaaaat aagttgtata aaatctggct actgtagatt
     1021 gacgatttct tttaaaatta caattataat aagttttata gaattaaaaa taaaacttta
     1081 gagatttttg ataacattgt agactaaatt tgccaccaaa atcgctttac agtggcaaat
     1141 cggtggcaaa tacaatatat tattagtata taatctatat aaacattact attgtttgat
     1201 tatcttagct acagtttcca catgtgtaga atgagggaac atatctacag gttgtgcctc
     1261 aacacattta aatccgtgaa catttagata atctaaatcc cttgctaaag tagaaggatt
     1321 acaagaaaca tagacaattc tttccggatt cattgaaaca atagtctcta ataccttttc
     1381 gtcacatccc tttctaggtg ggtctacaac tactacattg gcagtcttac cctctctata
     1441 catcttaggt acaatttcct ctgcctttcc aacataaaat tcagcattat gtatattgtt
     1501 taatttagca tttattttag catcttttat ggcatcatct ataatttcta ttccataaac
     1561 ctttttagcc ttttgtgcca aaaataaaga tattgtacct atcccacagt aaatatcaaa
     1621 aacagtatca gtatcattta aatttgcata ttcaagagcc ttatcataca atactttagt
     1681 ttggacaggg tttacttgga aaaatgataa tggtgaaatc tcaaacacta agtcacctat
     1741 ataatcgttt atttttccat ctccatatat aactatattt tctcttccta gaatagcatt
     1801 cgtatctttt tgatttacat ttacaaccaa agtcttaaat ccaggtatat tttccttaag
     1861 cactgaagct aattctttaa gatatggaag tttgtttcca tttgctacta atacaaccat
     1921 tacttcttta gttgtaaaac ctatttttgt tactaaatgc ctaatcaacc ccgtgtgagt
     1981 cttttcatca tatatactta ccttataggc ccttatatat gtttttatta ttttaacaat
     2041 tttatcattt atctcatgtt gtattacgca tttttctgtc gatattacat catgagtctt
     2101 ctttttatag aagccaataa ttggaatacc attattcttt tgtattggaa attgtgcttt
     2161 atttctatat ctatatggat tatccattcc tatagtgtca tgaacttcta catcctctaa
     2221 cttacctatt ctcgaaagta cttgtttaac ttcatttgtt ttcatttcaa gttgtttatt
     2281 ataatctaag tcttgtatct gacaccctcc acaatcccta agattgtcac tacacactct
     2341 tttcaccctg aatggagact tttctattat ctcaacaata tctcccacag cataattttt
     2401 ctttacttta ttaatcctaa ctttaacttt atcatgtatt aatccgccct ctacaaaaac
     2461 tgtaatacca ttgtactttc ctactcctac tccgccttga ccaatgtcta ctatatctac
     2521 aatatacatc tcgtttttcg aaagcataaa tcaccctcca atcaatctat tatatttaaa
     2581 tctttacaaa cttttttctt taaatttata ttaaaatatt gctattaatt aaactatatt
     2641 ttttactaag ctacaatatt ttattttacc aaaaaaaaag gagcttataa agccccttag
     2701 taaataaatt tattattttt cttataaaac tctagttgaa cctctatata ccacgcctct
     2761 tgatgcatcc acagttacaa cttctccatc ttttactaga tttgttatat cagctgcaga
     2821 cacaataact ggtatatcta aatttatacc tacaatagct gtatgtgatg tcattccacc
     2881 attctcagtt actacagctc ctgctttttc tatataattg ttcatttcag catcagtagc
     2941 agtagaaaca agtatgtcgc cttcattaaa gtctacacaa gcatttccat tctttattat
     3001 acgaacttta ccttctaccg tttgagtacc tacacctata ccttgcacga tttcttcgct
     3061 tattacatga acctttatta aattagtagt tccacttact cctactggta ctccagcagt
     3121 tataactact aattccccat tatctatata atcagcttgt ctagctgcct caattgcctt
     3181 ttctataact tcatctgtat ttcctgcaac agaagactta actggatata ctccccatgt
     3241 aagagctaat ttattcatag tcttttcatt atttgtagta gctattattg gagattttgg
     3301 tctaaattta gataccattc tagcagtata tcctgaagaa gtagatgtta ttatagctga
     3361 tgcatttaaa tctactgctg ttgtacaagt tgcatggctt attgcatctg ttacagtaac
     3421 attgttagta ccattttcct ttagtaatct atcataatct aaagtctctt ctgttctttt
     3481 tgctattgta gccatcattt ttactgcctc tacaggatat tttccagcag cagtttctcc
     3541 agataacatt atcgcatctg ttccatcata tatagcattg gcaacgtctg taacttctgc
     3601 tcttgttggt cttggatttc ttatcataga atcaagcatt tgagtggcag taacaactgg
     3661 ctttgctaat tcattacatt tttttatcat cattttttga actattggca tctcttcagt
     3721 tggtatctca acacctaagt cacctctagc caccattata ccatcagaaa cttttaatat
     3781 ttcatctagg ttctctacac cttcttggtt ttctatttta gatattattt gtatatcagt
     3841 tgcattattg ttctctaata cttctcttat tgctagtaca tcagatgcct ttcttacaaa
     3901 agaagcagct atataatcta ttccttgact tattccaaac tctatatcac ttatatcttt
     3961 aggagttatt gctggtaaat ttatcttaac accaggtaca tttactcctt tatgattttt
     4021 aactattcct gaattttcaa caacacaaac tatatcttct ccatttattt cttttactct
     4081 taagccaact aaaccatcat ctattagtat agaatctcct gatttaacgt cttcagcaag
     4141 acctttatag cttactgtac acatttcttt agtccccata atatctttca tagttatagt
     4201 gaacttttgc ccttcttcta aaaatacctc tgggtcttca aagttacctg ttctaatttc
     4261 tggaccttta gtatctaata atatagcaac tggcttattt aatttttctc taactttctt
     4321 tgccatatca attctttctt tatgttcttc atgcgaaccg tgagaaaagt taaatctaca
     4381 cacatttaga ccattttgca taagttgagt taaaacttcc tcactttgtg atgctggacc
     4441 tagtgtacaa acaatttttg tcttctttac attatttaac atatttgctc ctcctgagat
     4501 attattccac agatttattt ttaatactaa aacttatata gataatattt ttgccatctc
     4561 ataagcttct ttatcaaata ctttcttttg agctaaagct tcacttattt ctaaatcaat
     4621 tattttgttt tcttttattc ctacaactct agctgatttt ccatctaata ataattctac
     4681 tgctcttact ccaagtctac ttgctagtat tctatcagat acagttgggc ttcctcctct
     4741 ttggacatgc cccaatacag tgactcttaa atctgctcct gactcttttt taagttcctt
     4801 ttctaaatca ctagcacttc cgactccttc tgctaaaacg attatactat gtctcttacc
     4861 tcttttttga gtagtcttta atcttagagc aacttcatct actgtaattt ctacttctgg
     4921 tactattata gtttccgctc ctcctgcaag acctgcatat aaggctaaat ctccacagtg
     4981 tcttcccata acttctacta tgttaactct ttcatgagat gatgatgtat ctcttatctt
     5041 acctatagca tctataatag tattcattgc agtatcaaat cctatagtat aatctgtata
     5101 tgctaaatcg ttatctatag ttcctggtat gcctatagct gggaatccta agtcactaag
     5161 tttttgggct cctgcaaatg aaccatctcc accaattaca actaagcaat caattttata
     5221 ctttttaaga atttttactg cttttaatct accttcttct gttttaaatt cttcacatct
     5281 ggaagatttt aatatagttc ctcccctgtg tataatatct ccaacagaag ataagttcat
     5341 ttcagttaaa tcttcttcta gtaatccctt atatcctcta ttgattccat atactttgca
     5401 tccataatat attgctgatc ttacaaccgc tctaatcgca gcattcattc ctggagcatc
     5461 tcctccactt gttagtaatc ctatagtttt cattttttac ctccttggat tttatgtcaa
     5521 ttttttatta taatcattgt tattgtattc taatggactt tttttgtccc tatatcactt
     5581 tttaatccca tcaagactaa aaaaattaaa tgtccttaaa tgtccatttt gtgatattat
     5641 atcacaaaac ggacaatatt tttcatatta ttataatata tttagatttt tatataaaaa
     5701 atcaagcttt tacttttatg ttatctttag gaagtaactc ttctaagtta tttataaatt
     5761 catcacatat acaagtcaat gccccggtca ttttatatgc tttacgttta ccatttatat
     5821 tttcatccat agggtacaag tatatactgt cattacctgg atattgtttg gctatttcta
     5881 caatattatt agttgtatca gtatcagaca tactatctat tttcaaatac aattttttac
     5941 cactttctgg cttaatattt tctacatttc tagttcttcc tgaattgtag tttctatctg
     6001 caaagtcagt atcatcattt atatctttta tctctctagc tataagtttt gcattttcgc
     6061 cttcttttat acttaatgtt ccttttacat atattatatt atcttcattc aaaatgacat
     6121 tatacttttg taataattga ggaaaaacta ttatttctat agcaccatac aaatcttcta
     6181 attcaataaa tgccataatt tcatttcgtt ttgttgtttt aatagtttta gttacaatca
     6241 ttccacccat tataacatct gtttcattca tttgtaggta tgactcttct tcctctttta
     6301 atgaatttaa ttttccatta tccatagatg ttttttcttg tagttctttt tcaaattgtg
     6361 ataaaggatg tccacttaca tacattccca agacttcttt ttcaaggtta agtctctctc
     6421 tttcttcgta ctcttgcctt ttaggcaaac tgtatatttg ttgaaaatca ctatcttcct
     6481 cactatctcc aaaagcatca aataaagaaa tttgaccttg aacatttttc tttctatcca
     6541 tagatacact ttctaataaa ctttcataac cagccatcag actagctcta ttttcactta
     6601 tattatcaaa agctccacat tttattagac tttctattac tctcttattt gtatcttttt
     6661 ggtctaatct tttgactaaa tctactaaat ctacaaataa tccattagat tctctttcag
     6721 cgattatatt gtttattata tttacgccaa cattttttac ggctgctaga ccaaatctta
     6781 tattgtcgcc ttcaactgaa aactttgaaa aacttttgtt tatatctgga ggtaatacat
     6841 ctatttctag tgctttacat tctcttatat actcaactac cttatcagta tttcccatta
     6901 cactggttat caatgctgcc ataaattcaa ctggataata tgtttttaga taagctgtct
     6961 catatgctaa aaccccatat gcagctgcat gtgatttatt aaatgcatat ctagcgaagt
     7021 ctatcatatc atcaaatatt ttattagcta tttcctcttc tacaccattt ctgatacacc
     7081 ctgcaatttc tatattacca tcttcatcaa atttaccatg aataaaatat tgtctttctt
     7141 cttccatcac gtccatcttt ttcttactca tggctcttct aactaggtca cttcgtccgt
     7201 aactataacc acctaaatct ctaacgacct gcataacctg ctcttgatat accaaacaac
     7261 catatgtaac ttccattata ggctttaatt tttcatgtag atatgttaca tcttttgggt
     7321 tgcttttatt ttttatatat gcaggtatag aatccattgg acctggtcta aatagggata
     7381 ttcccgctac aatatcttca aaattgtctg gttttagctg tttcataaaa cttctcatgc
     7441 cagcactctc caattgaaac acccctagtg tatttcctga tgaaagcatt tcatatactt
     7501 tagggtcgtc ataatccatc tttgaaaagt ctattttttc tgtgtagttt tttataactc
     7561 tatttttttc tattaaatca agagtatctc ttataactgt aagagttctt agcccaagaa
     7621 aatccatctt taaaagccca agttcttcaa gtgttgtcat agtaaactgt gttgtgattg
     7681 catcttgatg tttgtacaaa ggtacatatt catctacggg attttttgat ataacaactc
     7741 ctgctgcatg agtagaagca tgtcttagca ttccctctat ctgttttgat atatttatta
     7801 tttcttttgt ttccgggtct tggtcataca attctttaag attaggattt gtatctaggg
     7861 ccttatctat tgtcattcct aaagcaaatg gtatttcctt ggctaccttg tcaactttat
     7921 tgtagcctat atccaatacc cttccaacat ctcttattgc agcttttgca cccattgttc
     7981 caaaagttat tatctgggct acatggtcat caccatattt tctctttaca taatctataa
     8041 cttcttctct tctctcataa caaaaatcta tatctatatc tggcatggat atacgttctg
     8101 gatttaaaaa acgctcaaat aatagagagt actttattgg gtctatatca gttattttaa
     8161 gtgtatatgc tactatcgaa ccagccgcac tacctctacc tggtcctact ataatattat
     8221 tttctttaga aaaatttata aaatcccaaa caattaaaaa atattctaca taacccattt
     8281 tttcaattac atctaattca taatttaatc tctctagtat ctcattgcta ggattatcat
     8341 atctctcttt taatccatta aaacacaact cccttaaata agtttctggt gtatatcctt
     8401 ctggaacatc atacttagga agatgtatag tgttaaaatc aaactccaca ttacacatat
     8461 cagctatttt cacagtatta tcaatagcct ctaatgcata tggaaataat tcttccattt
     8521 cttctcttga ttttaaataa aactcatcac taccaaatct cattctattt gggtcattta
     8581 atgtttttcc catttgaata cacataagta catcatgtat tttcgaatct tctttattta
     8641 catagtgaac atcattagta gcaactagtg gtataccagt ctcttttgat aattttacta
     8701 aaccagagtt tacttctttt tgctcaggta aattgtgatc ttgaatctcc aaaaagaaat
     8761 tttcttctcc aaatatgtct ctatagtcaa gagcaaattg ttttgctttt tcataatttc
     8821 tattcattaa agcacgtgct acatctcctg caaggcatgc agaaagtgct ataatccctt
     8881 tgttatgctt tttaagttct tccatgtcaa ctctaggctt atagtaaaaa ccttctacgt
     8941 atgaagttga aactattttt attaaatttt tatatccttc catattctta gctaacagaa
     9001 ctaaatgtcc ttgatatttg tcatagtttg ggtctttatc tctcaatcca cgtgcagctg
     9061 tatagacttc gcaccctata attggcttta ttccttcttt tatagctgtt ttgtagaaat
     9121 ctataacacc aaacatacaa ccatggtcag ttatagctat tgagctcatg tttagttcct
     9181 tagccctttt tataagtttt ttcaccctcg aaaacccatc aagtaaacta tactcagtat
     9241 gtacatgaag atgagcaaaa tctttgttca ttaaatcacc tcctgcttta taattttaat
     9301 tttaccacaa atatatgtac taaaacaaaa acagagactt ttatctcaaa ataggaatta
     9361 acttcaacgt ataccattat atgttataaa ttaatgctta ttctgagatt gtcccctcaa
     9421 tttattgcta tgattttttt ttcaaatctg ttgcaatttc ttctattttt ctaagtctat
     9481 gattgactcc agattttcct attggtggtt ctaacatctc tccaagttct ttgagagtca
     9541 tatcctcata ctcgattcta atcttagcta tctcttgtaa attttctggc aaactgctaa
     9601 ttcctatagt ctcttgtatg agcttaatat tttctacttg tcttactgct gcatttactg
     9661 ttttagaaag gttagctgtc tcacaattta ctatcctgtt tacattattt ctcatttctt
     9721 ttactatttt agtattttga agacttaaaa gagcgtggtg tcctcctata atactcaaca
     9781 aatctgatat ttgttcactc tctttgatat aaacaacgtg attatttttt ctagccacaa
     9841 ttttactatt aaaacctaaa gagtttataa gttcttttag agaatctgca aactcctcat
     9901 tattagttac aaattccata tgatagttct tttctgggtc acttatagaa cctccaccaa
     9961 gaaaagctcc tcttatgaaa gctctcttac atccttcatg cttaattaag ttctctgaaa
    10021 ttttgtttat agtaaaaaat ccttctttat cttcaagtat acctaacttt tttaataaca
    10081 tttctgaacc catttcgctt gttaccatta atacataact gttatttctc tttagcattt
    10141 gattcttatt tactgagatt gtagtattga tattaaaatt cttttttaat aatttaaata
    10201 ctttacgggc tattgaattt aactctgttg ttatctttag atttagcttc ttatatccaa
    10261 caatttgtat acttccccct gatttaacta gagctgacaa ttcagctata ttacaacatt
    10321 cattcccaga tataactctt gctagttcat ttttggtttc agttgaaaaa gacatatcta
    10381 tcacctatta actaattttt ctttaacctt ttttttattt cttgtaaggc aacctttatt
    10441 atctcctttt catcatcata cctagctaag tctactactc tatctaggtg gataaattta
    10501 gcatcaataa agccttcctc tttttgtgga atagtatcca tattctttgc ttgcatcaaa
    10561 taccaaaaaa ctgttttatg aactgctcta ttttcatccc agttttcttt gaatgtatag
    10621 tgtatttcac ctaaatattt caatatatct gcttttactc cagtttcttc acttacttca
    10681 cgtaatgctg cctcttggtt attttcccct tcttctactt ttcccttggg taacacccaa
    10741 tctccattga acttccttag taaaagaatc gtattgccaa acaggactac accaccagca
    10801 ctaacctctt ctctcatcga tgttcactcc ttaaaactaa cataatgata aggatttcta
    10861 cacaattttt taaaatcctt ttttactaac ttttgttgta attatgattt agggctaact
    10921 caataactat gttagatata tactttgcat catgtcttat ataattattt tttatttcaa
    10981 ttaaattctt ctctacagtt tttataccca tctcttttaa tttatctttt tgctttttat
    11041 ctaacaagac ttgctctgct ccatcttgtt tatataagtc aagcatttct tctggcaata
    11101 tctcattatt tgcgattaca taatctatta aattctcatc agtgtgtttt actatagcat
    11161 taacatgctc taatacatta tacccttctg tctcacctgg ttgagtcatt atatttgcta
    11221 tatatacttt tggagctaca gacgatttaa tagcgtctac aatcccctct acaagtaaat
    11281 tgggtattat acttgtatat aaacttccag gacccataat tataatgtca gcatcataaa
    11341 tagatgcaat aacatccttt aatggtttta catcttttgg atttaaaaaa attttatcaa
    11401 tagagctttt ttgaattttt gactcttcag gtatactaga ttctccattt attatattgc
    11461 cattttctaa tttggcaact aaatttacat cttccaaagt tactggtaaa actcttcctg
    11521 ttatagcaaa aatttcactt aatttatata cagctttttc aaagtttcca tacaacccat
    11581 tcatagcagc caaaaacaaa tttccaaaac tctgaccttt taaaagccct tctgtaaacc
    11641 tatattgcat aacttcattc attgttggct ctatatttgc aagtgctaat aaacagtttc
    11701 ttatgtctcc tggtgggagc attcctaagt cttctctcaa tacacctgaa cctccaccat
    11761 catcagcaac agttacaatt gcagttatat tttgcgtagt gtgtttaaga cctcttaaaa
    11821 ataccgattg accagtacct ccacctatca caaccacatt tggttctcta ttttcaacaa
    11881 tagacctttg aaatagtttt actttttcat tttttgcttt tttatatata aataacgata
    11941 caattaatgc aatccaacct aaaattccta caataataag taatttagcc atgtttgcct
    12001 ccattatcgc aacatagagt ctctatgttt ctttacaact ctatatccct tattagataa
    12061 atcctccgct ataagatttg ttatagtaac tgacctatgt cttccacctg tgcatccaac
    12121 accaattact agatgttgtt ttccttcctc tatatattga ggtactaaaa aatgaatcat
    12181 atctaaaagt ttcacataaa attcttcact gattttagaa ttcataacat aatctctaac
    12241 ttctttatca tcaccagttt tagctcttaa ctcttcaaca taatagggat ttggaagaaa
    12301 tcttacatca aatactaaat ctgcatcagc aagtatacca tgtttaaatc caaaagatac
    12361 tacagaaata gttaggtttg gattatcttc accagaagaa tatattttct ttatttcttc
    12421 cttaaggtct ttaggtttca tgttagttgt atcaattata catgtagata actcctttaa
    12481 aggttccatt atctttcttt ccatttttat accttctgga atctgcatat cttttgccaa
    12541 tggatgattt cttcttgtca ttttatatct ctttaacaat acatcatcat tacaatccaa
    12601 atataccatt tcatattcat aattttcttt ttctaaataa tttaagcttt catgaagcgc
    12661 ttcaaaaaac tttctccctc ttatatctat ccctagagca actttatcaa tacttgaatt
    12721 tggttgataa cataattctg caaatttagt tatcaaagca ggaggtagat tatctacaca
    12781 ataaaatccc atatcctcta aagctctcat tgtctcactt tttccagatc ctgaaagacc
    12841 tgtaactata acaaatttca ttgctaactc ctccctttta tcttatgttt attatattat
    12901 aatgttttct actaacgata tatccagatt agagtcagat atagtcttca tagctagttc
    12961 aaaatcacca actctttctg gacaatgtgc atctgagcct attataaatt tattttctag
    13021 cccttttatt ttatttaatt gatttacatt caaaaaagaa tggctactat ttatctcaag
    13081 tctagtattt gtcttttttg ctgtctttgc tacttcttct atatacacat cgcctttatc
    13141 tcctggatga gtaattataa aaatatcatt attacttatc gcatttatta cagcttttgt
    13201 attatattct tttgcttttt cagttttaaa aatatagtta ttacaatgat ttaataagct
    13261 tcttaatgat gttggagtag aaccaaaatg atatccagcc ataatataat caaaatctct
    13321 aataaattta tcatccatat ctatatttcc tttatcatct aatatgttac actccatacc
    13381 taaaagtata tttatatcag tgtacttatc atttaaataa tctacttctt ctctcatttt
    13441 gagaatatct tttttcttta caccatatgc aatatgttta taactatggt cagatattcc
    13501 tattgttttt attcctttag aaatggctac ctttacatta tcctcaatag tccctttgcc
    13561 atgactatat aatgtatgtg tatgataatc agccagtatt ttcatttgtt aatcctctcc
    13621 aagtattttg acttcttctt ccaacataac cccaaacttg gcatatacag cactttttat
    13681 aacatatatt aaatccaaaa tatctttagc acttgcttct ccttgattga ctacaaatcc
    13741 acaatgcttc tcagatactt gtgctcctct taaagtaaga cctctcaatc cagtttcttc
    13801 tattaattta cctgcaaaat atcctgttgg tctcttaaat gtacttcctg cactagcaaa
    13861 atttaatggt tgtttagtta ttcttctcgt cgctaaatca tccattaaac tttttatttc
    13921 atcataattt ccttcttgta attctaattc tgctgataaa gctatatatc cgtttttcga
    13981 tattatactt tttctatacc caaattccat ctgttcattt gataattcaa atatattgcc
    14041 ttccatatcc atcagtctta ctgacttcac tatgtccttc atttcaccac catatgctcc
    14101 agcattcatg gctaatgctc ctcccaatgt gccaggtatt cctgatgcaa actcaaaccc
    14161 ttttaattct tgcttttgga gagctttccc taaaactgat aataaagctc ctgactgagc
    14221 agtcatccta gtatcattta tctcataaga attgaaatta tcagctagtt ctattaccac
    14281 tcctcttatt ccaccatctt taattaaaat gtttgaacca tttccaatta ttaaatacgg
    14341 aatagattct tcttttacca acttaagtac attttttaat tgttcttcag ttctaggtct
    14401 aactaatata tctgctggtc ctcctaccct aaatgaaata tgttttttca taggttcatc
    14461 gacttttata tcctctttac ttaatatatt taataaattt atatatatat cttgattatt
    14521 cattgtattt cctcccaaat tatactgaac tttagctgtc attattattt aaattacatt
    14581 agtatatagt attaactata ttcaataata tatgattttg cttatcatat ggattttata
    14641 ttttaagctt cttaattaca aactatattc aataaatata taataaagta aaaaaaaaaa
    14701 aataacaaca tataaaagaa tataaataat ttaaaaaaag catcaatatc actgatatca
    14761 atgctttaaa aggcaattta attcgtatta tttatctaac tatttttgac atagcaaaga
    14821 taataaatcc ctccaactac tatggaacct ccaacgatat ttccaagtgt tacaaatacc
    14881 aaattagtta taaattgaac catagttata tttgctccta atgccattcc cattggtata
    14941 aaaaacatgt ttgcaacact atgttggaat ccacataata caaatagcat tattactgtc
    15001 caacatgaag ccaattttga cattatatct tgagatgctg tagccatcca tacagctaaa
    15061 acgactagaa tattacaaag tattgctttt aaaaacattg atgctatagt tgaattaacc
    15121 ttagcttctg ccacagctat agcttttgta gctgcatctc ctgtcaatgt agaagaataa
    15181 aacacaagtg caactagcaa aattgaacct actaaatttg caaaataaac cagtccccag
    15241 tttcttaaca tttttcctaa tgttattttt ttgtccataa gtgcaatagc cattaaggtg
    15301 tttccagtga ataattctgc tcctgccact actacaagca taagacctac tggaaagacc
    15361 atagcactta aaaagctagc tacccctggg tctatcttgc caaaagtttg actaactatc
    15421 atactcccca atgccccaaa acctataaat gctcctccaa ataaacctaa tagaattgcg
    15481 ttttgtgtcg aaatatttgc ttttttaatg cctgcactta tagttgcttg tgcagtttcc
    15541 ccaggtgtca agaatccttt actcataaat ccttccccct taaaaattat actttccctt
    15601 gtctttttat ggcaataatt atataataac aatatgata
//
LOCUS       DAEOSP010000084        15457 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.084, whole genome shotgun sequence.
ACCESSION   DAEOSP010000084 DAEOSP010000000
VERSION     DAEOSP010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15457
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.084"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            20..727
                     /locus_tag="KRH54_002039"
     CDS             20..727
                     /locus_tag="KRH54_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8579696.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            774..2369
                     /locus_tag="KRH54_002040"
     CDS             774..2369
                     /locus_tag="KRH54_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8579697.1"
                     /translation="MSNLSILIKNNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIGSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVKIFKNL"
     gene            2472..3554
                     /locus_tag="KRH54_002041"
     CDS             2472..3554
                     /locus_tag="KRH54_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBG8579698.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKEDKVNSNLDKVIEKLN"
     gene            3703..3864
                     /locus_tag="KRH54_002042"
     CDS             3703..3864
                     /locus_tag="KRH54_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBG8579699.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            4205..5473
                     /locus_tag="KRH54_002043"
     CDS             4205..5473
                     /locus_tag="KRH54_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG8579700.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            5668..6456
                     /locus_tag="KRH54_002044"
     CDS             5668..6456
                     /locus_tag="KRH54_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBG8579701.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            6456..6905
                     /locus_tag="KRH54_002045"
     CDS             6456..6905
                     /locus_tag="KRH54_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBG8579702.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            6909..9173
                     /locus_tag="KRH54_002046"
     CDS             6909..9173
                     /locus_tag="KRH54_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG8579703.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMSLFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            9313..9624
                     /locus_tag="KRH54_002047"
     CDS             9313..9624
                     /locus_tag="KRH54_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579704.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
     gene            9910..10410
                     /locus_tag="KRH54_002048"
     CDS             9910..10410
                     /locus_tag="KRH54_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBG8579705.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            10467..11453
                     /locus_tag="KRH54_002049"
     CDS             10467..11453
                     /locus_tag="KRH54_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG8579706.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            11503..12225
                     /locus_tag="KRH54_002050"
     CDS             11503..12225
                     /locus_tag="KRH54_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBG8579707.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            12254..14188
                     /locus_tag="KRH54_002051"
     CDS             12254..14188
                     /locus_tag="KRH54_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG8579708.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            14181..15449
                     /locus_tag="KRH54_002052"
     CDS             14181..15449
                     /locus_tag="KRH54_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HBG8579709.1"
                     /translation="MSKNIYVKETYEWIRIGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDDIKNTKNDLKKIIFNYKF"
BASE COUNT         6139 a         1439 c         2677 g         5202 t
ORIGIN      
        1 tataatatgg agggaaatta tgttaaaagt aatcaatgta tctaaatcat ataaaaataa
       61 aaaagtagta agtagtgtct catttgatgt aaatgatgga gagctttttg ggtttatagg
      121 acataatgga gcaggtaaaa ctactactat taaagcaatt gttggaatac acgattttga
      181 agagggagag gtactaataa attctaaatc tataaaaaaa cagcctgttg aatgtaaaaa
      241 ggaaatggca tatatacctg ataatccaga tttatacgag tcattaactg gtatacaata
      301 ccttaatttt atagctgaca tatttgaagt tgccaaagat aaaagagaag agttaataaa
      361 atattattca aataagtttg aactcaataa agcattagga gatttaatat cttcatattc
      421 acatggtatg aaacaaaaat tagctataat ttcggctttg atacacagcc caaaaatctt
      481 aattcttgat gaaccttttg taggtcttga cccaaaagct tcctttatat tgaaggaaat
      541 tatgagggag ttttgtgaca aaggtggttg tatatttttc tcaacacacg ttttagaagt
      601 tgctgaaaaa atttgtgata agattgccat aataaaagat ggaaatataa ttgcttatgg
      661 aactacagaa gaagttaagg gaagtaattc acttgaaagt attttcatgg aattaataga
      721 gaaataaaat gttttttata caatatagtg tctaaagtga ggaatgataa aatatgagca
      781 atctaagtat attaataaaa aataatataa taaatgaatt taaattaaat gcgttaaaaa
      841 aagctgatgg tagagaaaaa caaaaaataa tgggaatgat gctttcagtt gttataatag
      901 cgggtctatt aattttttat gtaacgagct taagtctctc tttagttgat atcttaaagc
      961 aaataaatca actggaaatg ctactgatat ttggttttgg attatcaaca ttaacaacta
     1021 ttgttacatc attatataaa acgtcatcat atctatttca agcaaaagat ttagaccttt
     1081 tgactagttt acctataaaa gaatctactg tactagcaag taagatatta atgttggttg
     1141 gagcaaacta tttattttct tcaatatgta tgattatacc tgcagtggtt tactttttaa
     1201 attctgatgt aagtataact tatttccctt atttgatatt gctattttta gctttaccat
     1261 tattaccgat agttgcttca tcaataattt ttctatttat agggcagata tcatcaagaa
     1321 taaaatacaa aaatttagta ttaatcatag gtagcattat attgatgttg ctttataccc
     1381 tacttatgtc aagacttaat tctatgtcag ttgaaatgct aaagaatagt gtgtctattt
     1441 cagagacgat tagaaaagta tatcctccta tatattattt tgttgatgct cttaaaacag
     1501 gaagtatagt ttcattttta gcatttatag ccatctcaat aataccattt tttatgtttg
     1561 taactctgtt tgcaaaaaaa tttaaagata taaatgctaa gatgagtgaa ggatataagt
     1621 caaaaaatta tgaatttaag ggtcaaaaaa cttcaagacc ttcaaaagct ttattaaaaa
     1681 aagaatttaa gagatatact tcttcgttta tatacttttt aaattcatct gcaggtttgt
     1741 tatttctaat agtaggtaca attttaatat tattatttgg agcagataaa gtgggtgagt
     1801 tactgaaatt gaatatagat tttaatctta taaaaataca attattggga gtattcttct
     1861 ttataatagt aatgaattgt acaacatact gttcaatttc tcttgaagga aaaaacttat
     1921 ggatactgaa atcttctcca atagatgaaa gagaaatatt ctggtcaaaa atattgatga
     1981 attttttatt gaatgcacca ctatctattt tatgtctaat attagtatct ataagattag
     2041 actttgaaat tcagtttatt attgtatcaa tctgtttgat tggaagtttt tcaatgtttg
     2101 tagcattgat gggattatta tcaaatttat tatacccaaa ccttgattgg aaaaatgagg
     2161 tggctgtagt aaaaagaagt gcaagtatga tagttacaat gttagtatca ttagcttatg
     2221 taattatact tggaggtata tatgtattac ttggaatttc gtttttaaat atatatatta
     2281 ttttggcaag tatacttact ttgatattag attttatgat atggagaatt atttctacta
     2341 aaggagtaaa aatatttaaa aatttataag tgtatttatg tcaaataaag acttatcttt
     2401 tttagtcttg caaataatag gttaagtagt atactatgta tagtgatatg aaaaaattta
     2461 ggagaaaata aatgagaaaa ttatccaaaa gagaaaaaga ttttattcaa tatggatttt
     2521 tattattttt gatatgtatt actacttatt tagtattaac tactttagat ataaaactaa
     2581 tacctaaaat tataaggcta gtaaataaga cttatatatt tcttggcata ttgacaatta
     2641 tagtatatat gataattgaa tctattgtaa ctcatttgat aataaatgca gttcaaaaaa
     2701 caaaagtaaa atttattggt attaaaatgg ctacaatggg attttactat aacttagtta
     2761 ctccatttgc atctgggagt caaccaatgc aaatatatgc acttacaaaa tatgatgtaa
     2821 gccttagtaa atcagtggct atagttacta ataaaactgt agtatttcaa agtacagtta
     2881 ctgtattttg tggaatttta attttgctaa ataagtcatt actgatggag caagtacatt
     2941 ctgtgaaagt cttgataact tttggaatga ttatgaatgt aagtatgttg ttaggtggac
     3001 tactaatagt atttagtcca aaacaagtaa aaatgatagc agaagttttg attaattttt
     3061 tatctaaatt taaaacattt aaatttttag attcaaaacg tgatagaata aatcattata
     3121 ttgatgatta taattattct ataaaaatat ttattaaaaa tgtaagaatc ttagttttaa
     3181 gtatattact tactgtgtta caattaatcg catatttcag tatagtttat tgtgtgtata
     3241 aagcctcaaa tttaagagga gtttcatatg tacatataat gacattacag gtctttttat
     3301 atatggctat atcaccaatt ccaacaccag gaaatgttgg agctaatgag gttacatttt
     3361 ttactatgtt ctccaatata attccaaaag aattattggg gtactctgta ctcttatata
     3421 gtgggtttgt atactatttt atattaatag tttcaggttt atttacattt ggaacgcatt
     3481 atacattaaa ttcttggaaa aaggaagata aagtaaattc taatttagat aaggttattg
     3541 aaaaattaaa ttaataaaat aattagggaa attctatata taaatatggt gttgaataag
     3601 ctatattata ttgagatatt tccctttttt gattaaaatt atagaaattt gaaataataa
     3661 gtaataaaca gaaaaagtga tattgttttg aaaggagaaa ctatgaaaca agataaacaa
     3721 aatgaaaaac aaagtgagat ttctaagata aaacaaagca atgataataa acttgaaata
     3781 gctcaagaat tacatgtaag taaaaatata gggaatatgc ttggatatag aatagataag
     3841 aaaaaaatga atagaccaag ttaatgaaaa tgttgaaaat ggtacatttt aaaaaatata
     3901 tataatgaaa tgttttattg ttaaatttaa acttatttta atggaggaaa aagtcgaaaa
     3961 atatctaaaa agatatggat ataaaaaata ttaatatata tatactataa acttgacaaa
     4021 atcaaccttt taataactag caaaaaatag caaaattata taagcgttat ttaattgtca
     4081 aaaatgaaag ctaaattgca tgtcctaaaa aaaatgattg ataacattta tttgtgtgct
     4141 ataattatat agtaaaacaa taagtatatt gcataatgta tgcaaatcgg gaggagagac
     4201 ggacatggaa gcatctatac acacaattgc cagcaataat ttattgataa tttttgctat
     4261 tgtaagtata actggaataa tttgtagtaa gcttagtgaa ataataaagg ttccagatgt
     4321 agtgttatat ttacttgtag gtattttaat aggaccatca tttttaaaat ttatagatat
     4381 tcgtggattt caaatagaaa atcaactaat acttacattt ggttctgcat ttattttata
     4441 tttaggtgga aaagaaataa gtttaaaagt attgagaaat gtgaagatat ctgtattctt
     4501 attagctact ttaggagttg tgatatctgc ttttataatg caacaagtaa taggatttac
     4561 ttttgggata agtgcaatga ctgctttact agcaggaact ataatagcat caacagaccc
     4621 agccactctt gtacctatat ttaatcaggt aaagataaag gatagggtaa aacaaactgt
     4681 aataagtgaa tcagctttta atgatgcaac aggagctata ctaacatcag cagtgttggc
     4741 tgtaattttg tcaaataaat tttcattagg tgaaaatatt tatgaactgg ggacaatggt
     4801 tattgtcgga gttttagttg gtttaattac aggaatactg cttttgaaat tagttaatga
     4861 taaaccttat ggaatattta aagattttgc accaataata tctgttattt cagttgtagt
     4921 tgcatatgaa ttatctacaa aattaggtgg aagtggatat atgtcttgct ttattgtagg
     4981 tataataact ggtaacaaga aaaactttaa aatttggtta agtcaaaaat cttatgatgc
     5041 agatttttat gttgctgaaa ctttaggtac tatttgtcgt atggcaatat ttataatatt
     5101 gggtagccaa gttaatttgg ctcagttaag taaatatttt atgccatctg taattacagt
     5161 attagtatta atgtttatag caagaccact ttgcgtttta gtttgtacac ttgtagacag
     5221 ggaagcaaaa tggagtaaaa gtgaaatatt atttatgatg tgggtaaggg aaacaggcgt
     5281 aataccagca gcactttgtg gtattatttc agcaatgaaa gttcctggat acgaagtgat
     5341 ttcatctgta gtattcatga ctatactaat tacattagta atacaaggaa gtactacaaa
     5401 attagtagct aaaaaacttg gattattaga agaagaagtg ataaatataa gtgaaaaagt
     5461 atcaactttt taaagttttg ctttaaaata gtacctgtta atggctattt taatattgtc
     5521 taatgatgat tattgaaata gtcatttttt tatgtataag taatttacag aatttacata
     5581 ttagacatat ttattatcaa aatttgttga agctatgata taattagttg tatttaaaat
     5641 aagaatagca aataagggag gaaatttatg tttacggtta tggatattgt acagccagat
     5701 acagtagaag aagcgtactc aattttaaat aaaagaaaaa ctaaccaagt aattggtgga
     5761 agtgcttttt taagaatggg gaaaaaaaga ataggaacag gaatagaatt atctaaatta
     5821 aatttagatt atataaaaga gtatgaagat tacgttgaga ttggttctat gactactttt
     5881 agaacattag aaacaagttc cataataaaa aataattttg gaagaataat agaagattca
     5941 gttaaaaata taataggagt tcagtttaga aatgtagtaa cagttggtgc aacagtattt
     6001 tctaaatatg gtttttctga cttgatagta gcattacttt cattagatac agaagtagaa
     6061 ttatataata ttgggagaat tagtcttgaa gagtttctaa atagagatta tgagaaagat
     6121 ttacttatta agatatacat aaagaagaca aataagaatg cttcttataa atcacttaga
     6181 aatgcaaaga gtgattatcc aattctaaat gtatctgtat caaaacatat ggaacaattt
     6241 aagttatgtg taggagcaag accgcagaaa gctacgattg caaaacaagc tagtgaattt
     6301 ctatctaaca atgaaatcaa tgaaattaat atagataaag cagtagaaat agcatcagaa
     6361 gaactaactt ttggttctaa catgagagca tctagggagt atagaaaagc tatgagtaaa
     6421 gtacttttaa aaagagctat aatggaggtt atttaatgtt agtaaagatt aatataaatg
     6481 ggaagaaaaa aagtgttgat atagaaccag aagaatatct ggttgatact ttaagaaaag
     6541 ttggtaattt aagtgtgaaa agaggttgtg acacaggctg ttgtggttta tgtacagttt
     6601 taattgacaa gaaacctaca ttatcctgtg caacactgac attgagagct ttagataaag
     6661 aaataactac aattgaaggt ttagaaaatg aagttaaaga atttagtgaa gttttggtaa
     6721 aagaaggagc tgagcaatgt ggtttctgtt ctcctggctt tatacttact gtacttgcaa
     6781 tgaaggatga gcttgaaaat cctacagatg atgaaataaa gcattatctt acaggaaatc
     6841 tatgtagatg tacagggtat atgggacagt taagagctat caaaacttat ctggaggtaa
     6901 aatagaacat gaatatagtt ggaaaaggaa tcaagaaaat agatggaatg agtatcacaa
     6961 caggaaagcc tgtatataca gatgatttat cagcaaaaga tgctttggtg gtaaagatac
     7021 ttagaagtcc tcacgcttat gcaaaaataa aagatataga tacatcaaag gcagaaatgg
     7081 ttgatggtgt tgaatgtgtt ttaacatata aagatgtacc aaaatctcga tttacacttg
     7141 ctggtcaaac atatccagag ccatctccat atgatagatt aattcttgaa gatgttgtaa
     7201 gatatgtagg tgacgaagta gctatagtgg cagctataga tgaaaaaact gcaattaaag
     7261 caatgaatct tatcaaagta aagtatgaag tacttacacc tgttttggat tttgaagagg
     7321 caataggaaa taaaactatg gtacatgatg aagatagaca ttgtaacttt gatataggta
     7381 tgcaaagaga aaaaaatata gtatcccatc atgattatac aaaaggtgat gtagaaaaag
     7441 tattaagtaa ttgtgatgta gtgattgaag aaacttatta tactcaagct caagcacatg
     7501 caatgatgga gacgtatagg gcatacaatt atttagacca tatgggaaga ttggttgtag
     7561 taagttcaac tcaaatacca tttcatgtaa aaagacattt atctcgtgca ctagaaatac
     7621 cagctagcaa aattagggtt ataaaaccta gaattggtgg cggatttggt ggtaaacaaa
     7681 ctgcatgtgt tgagattttt tctgcaattg ttactttaaa aacagggaaa ccagctaaaa
     7741 taatttatga tagaaaagaa acttttaatt gttcaacaag tagacatgca atgagattaa
     7801 aagttagact aggtgctaca aatgatggca taataaaggc aatagatata aaagcacttt
     7861 ctgatacagg agcatatgga gagcatgctt ctacaactat tggtttggtt ggagaaaaaa
     7921 ctttaccaat gtataataaa gttgaagcaa caagatttat tgcagatgtg gtatatacta
     7981 ataagatgcc agcaggagca cttagagggt atggagcatc acaagggtgt tttgctgttg
     8041 aatcaactat aaatatgctt gctaataaat taaaaatgga cccaacagat ttaaggttaa
     8101 aaaatgtggt aacagaaggt gaaactacat ttgcatatgg taaaacttta aatagtgtcg
     8161 atttaataaa atgtataaat aaaggcaaag aacttattgg atggaatgaa aaatatcctt
     8221 gtaaacatct tgataatgga aaaattagaa gtgtgggtat ggcaattact atgcaaggtt
     8281 ctggtataca aggtcttgat aagtcttcag ctgaaataaa attgaatgac tgtggagatt
     8341 atactcttat gataggttct actgatatgg gtacaggaag tgatacgatt ttagctcaaa
     8401 tggctgctga ggttcttgat acaagcattg aaaatataac agttatatca gctgatactg
     8461 acttagttcc atatgaccca ggttcatatg cttcaagtac tacttatgta acaggtatgg
     8521 cagtagttaa ggcagctcaa gacttaagag aaaaaataat ttctgctgga gcaactaaac
     8581 taggtgttga gaaggatgaa gtagaatttg atggtataaa attaactagt aaaaaaggta
     8641 gcactttatt attcgatata gcagttgatt ctacaattgg tccagatact aatcaattaa
     8701 taggtcttgc ttctcatggt agcccagtat ctcctccacc ttttatagca ggttttgcag
     8761 aaactgaaat tgacacagaa actggaaaag ttgatttagt aaattatgta gctgtagtag
     8821 attgtggaac ggttataaat aaaaatctag ctaagataca agttgaaggt ggaattgttc
     8881 aagggattgg aatgtcatta tttgaagaag ttagatacac agaaaaagga agcatggaca
     8941 caaatacatt tatgcaatac aagattccag ctagatgtga tattggaaat gttaaagtag
     9001 attttgttga aacttatgaa ccaactgggc catttggtgc taaatctgtt ggagaagttg
     9061 ttataaatac acctttacca gcaatacaag aagctgtgtt taatggaatt ggtgtaagaa
     9121 taaatacact ccctattaca cctgaaaaag tttttatgga aatgaataaa taattattta
     9181 tgaaaattta gactagtttt acatgttaaa caaccagtta tgtcatacct gttgaaagat
     9241 atttaataag tagttattat aatattatga attttactta taaaatacaa aattaaataa
     9301 ggggatgatg ttttggctgg taatctaaat aatatgagag cagtaaataa ttttagagga
     9361 gataagaaca ttttagaatg tttagtcagc tttgagggtc gttcaataag tcagagaaaa
     9421 gtaagggtat tttttaaaga aaaacaaaat caaatagaaa ttgattttgc agaagaggaa
     9481 atttctaaat tggttgaaaa tgttgtttta aatacatcat atcaagaaat gttatatgat
     9541 gaaatagaga aacaactgga aattgattgt ataggtactt ggatgatatt atctaaatta
     9601 aaagatggta gtagagttca ctgattttaa aataaattat tagagcctaa ttttaaggct
     9661 cttttttgct gtttttaatt aacaatagaa aatttatctt tagtttgtat ataattttaa
     9721 tatcatatat tctatatata taaaattgaa tttgtatgta ttatattgta taatataaaa
     9781 tgaattggga aaatagttcg ctaaaataat ttgtttttat aaaattactg tttaagttta
     9841 tagattttgt ataaaataaa gttatgttat taattttttt aaaaataaaa cttttactag
     9901 gagacagata tgagaaaaaa tagtattaat atagcactaa ctgcagcaat tacagcaatc
     9961 tatgcagttt taacattatc attgggattt ataagttatg gaccaataca atttagggta
    10021 gcagagatta tgatgctttt ggcattttta gataaaggat atatagtagg tcttacatta
    10081 ggttgttttt tagcaaatgt aataggacca tatggtgtac ctgatataat ttttggaaca
    10141 ttagccacat ttataagtgc aagtatgatt tatattacta gaaagttagg taaacagaat
    10201 aaaagtactt tagttatagc ttctttatgg ccaactgtta taaattcgat tgtggttggt
    10261 ctaatgttaa atgtattctt tgggcttcca ttaattctat ctatgataca ggtaggtttt
    10321 ggagaatttg tagtagtaac aattgtagga gtaccatttt tctgtttttt aatgaaaaaa
    10381 tataaaaatg tcattaatat taaattttaa atagtgtata gtaatttaat taattgagag
    10441 tattattagt gaaaagaggt gtttatttgt cgaaggtaaa aagattgact gatgtttgga
    10501 tatatattaa tgataagcaa aagtttacaa taaaagagtt gtcagaggaa tttaatcttt
    10561 cagccaaaac aatacaacga tatttaaaag aattaaataa aatgggactt ccaattcaag
    10621 cagaacaagg tagaaatgga ggttataggg tgctaaataa tagctatata cctcctgtta
    10681 tatttacaga aaaagaagtt atgattatac tatttgcact taagtcactt cagatttata
    10741 attataagtt aatggaaata gaaattaatt ctataatgag aaaagtaagt tatgaacgta
    10801 aaagcagtat aaaagtaggt atagaaaata ttaaaagata cattggcttt gtagctaatg
    10861 atactaatga tggagatttt aagagtagta atgtaactga gttttttaga gcttcttcag
    10921 aatctctaat tttaaatatt aagtataagt gtggtaatca gattttggaa aaaaacgtat
    10981 gccctatagg tatatattta aataagggag catggttttc accagcatat gattgtaaaa
    11041 gtgataaaat aatattagtt catatagata atataattga tataaaaaga acaggagaaa
    11101 gtaaaaaaat acatataaac ttggaagaat ggttacatga agtatataac aatttatatg
    11161 gaaatagttc aactactaaa aaatttggtg aaattattta tctaaatgta ttattaacta
    11221 aagaaggtgt ttctaaaata aaggacagtt cttggaattt agatatggag ctaaatgaag
    11281 atggtagtgg aataattagt acattcataa aggttgaaga tatagattgt tatgcttcga
    11341 ttttattttt tattggaaat aatgctaagg ttatagagcc aaaatttttg aataagctac
    11401 tttatgataa ggccaatgag ttaagatatt tttatgaaga aaatatggta taatatttag
    11461 aaatgttgaa attaaataat tattaaggag atatacgtta taatggaaaa ttttaataag
    11521 aatgctaaaa taattggtat tggagctgaa ggtattaata taataaatga agtagaagaa
    11581 aaaataaaag caaatatgga tatagaaaaa ataaacataa atcaagagat tgaaaaagaa
    11641 tatgttagaa gtcttttaga tggtgtagat atattatttt tgatatattc atctgaagat
    11701 aaacatatta gagatattat aaaagctgtt tcatatatgt caaacgaaag aagagttcta
    11761 tctataggaa tggattgttc agagaaagaa aataaagaag atttagagct tgggagagag
    11821 tttaaaatca ataatgatag tatatttaaa tttgttgatt taatgaatat catggtagag
    11881 tctatttctg attcttgcat gataaatata gatattactg atttaaaaga agctattgta
    11941 ggagataaag gaattaagta ttcttttgaa gagtttgaag atacaaagtc atatagcgag
    12001 atagctgaca ttttatttga caggatggaa tatattggtg aggaatttat aagtaagaaa
    12061 ggaattgtat ttgttgaagg aagtccagaa ttttctataa tggaattgaa tgatttaata
    12121 agtaatattc agagcaaggt agaagaaagc tatgaagtaa tattttcttt atatataaag
    12181 gaaaatttaa atggaaatat tagagtaggc ttattgtata attagtatat tttagatatt
    12241 aaggagagtg tatatgtctg aaaatataag aggtacagag tattggttaa tggaagaatt
    12301 tttttatcag gaaacagaca caccacagat ttttagagaa gaaagcataa ttgcacttgg
    12361 atttgatata aaaataaatt taaaaaaagt attcagtatg gggaaaaaat cagaaaagta
    12421 tattaatgag aattgtaatg ataaagattt tataaaaaag tttatgtcga ttaaaaaagg
    12481 agattatttt ttatataaag catatcaaaa tatagaagaa aaagttaaat gtaatgtgtc
    12541 tattggcata gttgaagaag attttgaaaa tggatatgaa ttaagtcata ttttagggca
    12601 tacactacca gtaaaatgga tgcataattt tgatacagtt tcaaattgta aagtatatac
    12661 aagtgaattt tataaattaa ataataatga tattagctca tatttagaaa ttattaagtc
    12721 acaagataag aaattattga gaaaaagaat agaagaaagt gaaaataata aatggtattt
    12781 aaaaaactat tattataaaa atgtagataa gtgtgtagat gatgaagaat tatttattca
    12841 gcccattaat ctagaacaat tagaaagtct aaaaaatata aagacagatg attatatttt
    12901 attgtataat agcgagaaag taatttgtga aaatgaccat acaatgaaga gtctatttgg
    12961 cgtatgtcaa gttatggaag ggttcgataa agagaaagta agtgctttgg agggtggata
    13021 tttttttcct gttagatgga tagagtatga aaaggagaat atagacggaa atgcatgggt
    13081 tatatttgaa aataatagta tagttgaaaa gtatgtaaaa agatttacta ataaaatgcc
    13141 attaaatact atactttatg gaccaccagg aactggtaaa acatacaata caaaacagat
    13201 ggtttacaat ctagtaagtg aaaccagtct aagaaacaga tactatacag gttacttaga
    13261 ttataaaaac aatgtagaat tttgtacatt tcatcaatca tatggatatg aggaatttat
    13321 agagggtctt aagtctgatg gtgaaggcaa ttttaagcca gaagatggga tattaaagga
    13381 tatatctata gaagcatgtt ataatgcatt gaaatttgaa aaaaagatga gctttgagtt
    13441 ggaaaaagat agcttgactt tagaaactat aaaagacagg aaaaagaaat tagtattaga
    13501 aaatatagaa tatagtgaga gttttaattt tgaagaatca gaaaactatg ttttaataat
    13561 agatgaaatt aatagaggga atgtatctaa aatatttggt gagctaataa cattattgga
    13621 agatgataaa agacttacta aatcaaatca aactattgtc aaactaccat attcaaagga
    13681 aaaattttct ctacctccaa atttgtatat agttggaact atgaacacat cagataaatc
    13741 aatagcactt ttagatatag ctttaagaag aaggtttaat tttgaagaaa ttatgccaga
    13801 ttatgattta ttgtctattg tagatggtat agatattaga aaaatgctat atacaatcaa
    13861 taaaaggata gagtttttat atgatagaga ccatgttatt ggacaagctt atctacttgc
    13921 tgtagaaaat attaatgata tagtagtagt atttagaagt aaaataatac cacttcttca
    13981 agagtatttt tattatgatt cagaaaaagt aggtttgatt ttaggaggaa ttggaagaaa
    14041 tgaaaaagat aaatacattg tttataaaga aaaacttata gcttctgagt tatttaaaaa
    14101 taataatgcc tcaataatgg agagtaaagt taactattgt ataaaacaaa acatatctag
    14161 tgaggagcta aaaaatatat atgagtaaaa atatatatgt taaggaaacc tatgaatgga
    14221 taaggatagg taatggtgaa aatgagctta cagaaattga atatgaaaaa ttgcttaagt
    14281 atctagagaa caataatgat gtattaaaaa gtaatataat agatattaaa tacaaaaaac
    14341 ttagatttat aaactatgta ggcatcatat gttttgaaaa tgtaatactt gaaatacttc
    14401 ctaaattgtc attaagtgat aatcttgtaa aagatagaga gatactatta cagatgcttt
    14461 ctatatgtaa taaaattcca attacaatga atgaaaaaat aagattgagt ttgaaaaatt
    14521 ataatctctt aaattttttt gttatgtatt tcattgagag tatgcaaaca cagatgaaaa
    14581 aaggtattta ttttgaatat ataaataaaa tagagaactt aaatgttatg aggggtaaaa
    14641 tattattaag tacatatgca aaagaaaaag gcatttctcc aatgaaaata agatgtgaat
    14701 atgatgagta tagtgaaaat aatttcttga atcaagttct taaaaaagca tgtatatcta
    14761 ttttatgtag aataaatgac aattcaattc aaggcaagat taaaaaaatt ttaagctatt
    14821 ttcaaaatgt agatttaatt tatatagata gaaaaaaact attggattat aaattttata
    14881 aaaacaatga tagatttaaa gattgttatt tactagctag acttctactt ttaaatttgt
    14941 ctatggataa tagtcaaaat aatcaagagg cattttctat attatttgaa ataaatactt
    15001 tgtatgaaga atatatagga attttaatca aaagtatatg ggacaattca tttagagaaa
    15061 catatataca agataagagt aaatttcttt tgaaaaatga acaaacaggt aagaaaaact
    15121 ttaatttaag accggatata gttttatatg acttaaaaaa tgaatatgaa attataattg
    15181 acactaaatg gaaagctatt gaagtagatt caaatgtttt ttataggtca agtgatatat
    15241 accagatgta tgcatatata acagcatatg aaaatgcaaa aagatgtata ttattatatc
    15301 cttgtattca aaaagataag aattatagtt cctggaaatt atcagaatca tttaagggta
    15361 agtttataga agcaaaaact gtgaggttag atgatataaa aaacacaaaa aatgatttaa
    15421 aaaagataat ttttaattat aaattttaaa aataaga
//
LOCUS       DAEOSP010000085        15403 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.085, whole genome shotgun sequence.
ACCESSION   DAEOSP010000085 DAEOSP010000000
VERSION     DAEOSP010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15403
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.085"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(228..1052)
                     /locus_tag="KRH54_002053"
     CDS             complement(228..1052)
                     /locus_tag="KRH54_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG8579710.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPDKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIELINYYAMLYLIVAIVLVAINPGNALYKRFSKNTTQIL"
     gene            complement(1156..1950)
                     /locus_tag="KRH54_002054"
     CDS             complement(1156..1950)
                     /locus_tag="KRH54_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8579711.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            complement(2484..3338)
                     /locus_tag="KRH54_002055"
     CDS             complement(2484..3338)
                     /locus_tag="KRH54_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBG8579712.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENVESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            complement(3370..3882)
                     /locus_tag="KRH54_002056"
     CDS             complement(3370..3882)
                     /locus_tag="KRH54_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBG8579713.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTVMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            complement(3875..4099)
                     /locus_tag="KRH54_002057"
     CDS             complement(3875..4099)
                     /locus_tag="KRH54_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579714.1"
                     /translation="MNEKPSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            complement(4159..5112)
                     /locus_tag="KRH54_002058"
     CDS             complement(4159..5112)
                     /locus_tag="KRH54_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG8579715.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEVIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            complement(5140..6528)
                     /locus_tag="KRH54_002059"
     CDS             complement(5140..6528)
                     /locus_tag="KRH54_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579716.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFTPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            6802..7683
                     /locus_tag="KRH54_002060"
     CDS             6802..7683
                     /locus_tag="KRH54_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG8579717.1"
                     /translation="MNTKQLEYFISVAEKLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            complement(7871..8863)
                     /locus_tag="KRH54_002061"
     CDS             complement(7871..8863)
                     /locus_tag="KRH54_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBG8579718.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKALSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEGMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIGIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(8868..9608)
                     /locus_tag="KRH54_002062"
     CDS             complement(8868..9608)
                     /locus_tag="KRH54_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBG8579719.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            complement(9673..10782)
                     /locus_tag="KRH54_002063"
     CDS             complement(9673..10782)
                     /locus_tag="KRH54_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBG8579720.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDVVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            complement(10800..11933)
                     /locus_tag="KRH54_002064"
     CDS             complement(10800..11933)
                     /locus_tag="KRH54_002064"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBG8579721.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELKRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIINKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIEENE"
     gene            complement(11935..13029)
                     /locus_tag="KRH54_002065"
     CDS             complement(11935..13029)
                     /locus_tag="KRH54_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBG8579722.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNEYQVVFNVVAEEKTSEVEKIIMVNEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNKNLFKRGDVVIGNNDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            complement(13166..14467)
                     /locus_tag="KRH54_002066"
     CDS             complement(13166..14467)
                     /locus_tag="KRH54_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8579723.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            complement(14692..15393)
                     /locus_tag="KRH54_002067"
     CDS             complement(14692..15393)
                     /locus_tag="KRH54_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8579724.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
BASE COUNT         4893 a         2866 c         1682 g         5962 t
ORIGIN      
        1 ataataaatg ttccttataa ttttaatatt ttaaaccaag aaagttaaag tttctctcat
       61 ataataagtt gatacttatc tatttcattc ttttaagtgt ttcatctaat gaaatacact
      121 ctgcatacct tccattccag ataaatctat tgtaatattt attaattaaa attaattaat
      181 aaggagaatt atttctctca tttgtgatat gattctcact tatattatta aagtatttgt
      241 gttgtgtttt tagaaaatct tttatataag gcatttcctg gatttatagc taccaataca
      301 attgcaacta ttaaatataa cattgcatag taatttataa gctctattaa atctccatta
      361 attatcccta ttaaaaaatt aaatagttga tgtattacaa taggtattat caaagtttta
      421 tttagattat aaaaagttgt cattataata ctgattgata taattgcaat cataaaaaat
      481 ataatatatt ttattaaatc aagtcctttg aatcctgttg taaaccatat tggtaaatgc
      541 cacatccccc accaaaaacc tactattatg gatgctttta aaggagaatg tcttttctga
      601 agatgatttt gaacaaatcc tctccaacct aattcctcac ctaatgatcc tgcaaataaa
      661 ttttttaaaa ataaatatat aaatgctccc catgatgaaa ctgtaaagtt aggcattatt
      721 ccatgatttt tagatgctaa tataaatata actactacag ctattaggac ttgtatacta
      781 attattatag atactgttga aaatttaagt ttatttctaa atctatcttt tacgtgtact
      841 aaaaatttct tatctggata aattttttta aataatatca taaatgcaaa agtggatgac
      901 cacgctgata tacacaacgc aatgtcagat atcactgttg gaagttttaa cattatagtc
      961 actactacca ataaaaaaag tggtaaaaaa attatatttg ttagaataat aaaactggct
     1021 ataggccttt ttaatgattt ctctgttctc ataagtaatc ctcatttcaa attaatattt
     1081 aattgctata taagttataa ctgcttatat catgagttta aacctatgag taagtcccag
     1141 gtcaatgctt ttatattatt ttataggtgc ataaatctct aaaaatatac gctcaatccc
     1201 ttcttcataa gtagtaagca atatattaac atagactatt ccaagtaatt cacattcatt
     1261 ttctattgct atttctctta atctgtcttc cactttatta tcaatatttt ctcctccagt
     1321 tgaccatctg ccatcttcaa taatagtata aatacaattg ggatgagata gaacttcacc
     1381 ttctatgatt ttgttgtagt catctacttc ccttacaatt ataaattttt cttctattat
     1441 accttcttca ttaaaattta ttactcttct taaatcagtt aatgtaactg ctttttttaa
     1501 gttgtttgta ttctctttta gggcttcata ttcctcatag ttagtaacat gttttatttt
     1561 accttttact tccaaaggct tcatttcttt tattgtgtat tttccaaaat atgtttttat
     1621 tttgtcacaa tcttctttga ctaactgaac cttttttaag agaagctttt tatattctat
     1681 ctcttttagt atgatttgtt ccttctcttc tagtaagagt tctaaattgt ttatactctt
     1741 actttttctt atttcttgaa tctttttaat ttctatatca atttctctat aaaagtttat
     1801 tgttgcaata tcatagatat caacctgatt atatattcta tatccatttt catcattttt
     1861 cttaggattt actaatcctt tgtcctcata aaattttagt gtatctctag atattcctaa
     1921 aaatttagat acttgtccaa ttgtatacat tatattctcc tattattaga taaattcttt
     1981 atgctcatta aacgtatttc tattaacaat tacaaaatca cctaaacttt gaagtatcaa
     2041 agtcagcata gtatttacaa cctttggaat tactcttttt atttcataat ccaagtgtaa
     2101 acttgttaat atgatacgtt ataaagacta tattgtctac atatttctaa caatgattct
     2161 ttcactttaa gatgttctta aacatattta acttttattt ttaaaaattt cctatacata
     2221 aaaatattcc tttcatgata acagttttat tatttcaact atctgctacc ataaaaggaa
     2281 tatcttaatc tttaaacaca ttctaaatgc tcttttaaca aatattatca aattattcta
     2341 attctattgt ttcatattta ttagtcttag ttttatataa atttaaatat aaaactatat
     2401 attcacctac ttttaaggat acaataagtc ttcttctacc agtaagagta aatgcctcct
     2461 ctacttatca caaactataa tttttatctc attggtatat atccacgata ctgtgagtat
     2521 gcatccatag ttgctatatc acctaagaat actgatgcga tattgaattg tggttttaaa
     2581 tatgggaact cctcttcttg tgcccattct ggttttttaa taaacgactt tgtctcttca
     2641 accacagctt tataccattc ttcttcccct tcaaaccata gttctgatac acggtcaaaa
     2701 gcagttgctc cataattaat cattatttta ctactgacaa aacgattaac atagcaacag
     2761 tttgtaaagt atggcactac ctcatcatag aaccattttt ctccttcttc tttagagata
     2821 ccatctggat acttaatcat aaattgccaa cgatagtttg gtccatcttg tacagtacgt
     2881 ccttttcctc taaagtcttc ttcccagttt attggaacaa aggcaaatat aaatggtgga
     2941 catccattat ctccacctac tgcacgtcct gcatctccac tttctacatt ttcatgaacc
     3001 cttttatttt ctacatctgg tatacaccct tgccaacgta gaacatccat aggcatatat
     3061 tctgtcataa ttctatttgc catctcaggc atatgttcat ctacaagcca ataatgttct
     3121 gttagttgca tcttacgtgc accaaaacgc tcaccttcat ttggtgtagg atatgcttga
     3181 taaaaggcat atttagtaca atatggtcca aaagtgctaa tactatctgg aatatggtga
     3241 cgatataacc agtccattag cttaggtcta tattcttcct tggcaagtcc tacatatatt
     3301 aaacttctca ttattttaaa ttcaaaaccc atagccattc tcctataatc aaagtatatt
     3361 cacatgcttt caatttccct ttttcctatc tttactcaaa atatatattg taaacaaatc
     3421 tacacctgca cccacgtacc atactcctac tgccatagct acagcaattt gaatacaaat
     3481 atcttgtaaa ttaataatat tttcacctgt cataacagtt agacctatag cccaaccaca
     3541 aagccatgat ggtaggaaaa ttattttttc aaataaggat gcaattacaa ctgcaaaaaa
     3601 acctaatatg ctaagtgtta aaaataattc taggttttga ttgaaattca tatatcccat
     3661 aattaaaatg gctaacactg cccataaatc tccacaccaa aaaccaaaca ttatttttaa
     3721 tccatctttt tgtttattac cattagttac atataatcct gcacagatta gtgctacagc
     3781 tccagttttt attcctatat aaggtgctaa tactgcccat aaaggaggta gtaaagcaat
     3841 ccccatagac agtgttaata tttctttttt ctttctatcc atttcctata ttgtaattag
     3901 tagcttccct agcttgcctt atctccaata gcttaattgc tatttgattt acaatcatag
     3961 atactgcaaa ataacttaaa tatagtggta taaatataac aataaatgct ggagttaatc
     4021 taaccttgta atatgtaagt accaaaaaaa taactgctaa aataacttca atttgtttaa
     4081 aacttggctt ttcattcata caataagcct cctataacat ctaacatccc ttaaaaaaga
     4141 gtcttagtat tttaattatt atcctaatga aactccagct tgcttttctt tagttaatgg
     4201 ctctataaaa ttataagatg tttttctttc tttaaattct tgtgttttac gaacttctgc
     4261 ataatgacct tccattttct taatatcatc accatgtata aacttacctg gtaaagtatc
     4321 atgagcatga cctaattgct cttctggaaa actaaatata gtttttccac ttatagtatc
     4381 taactctcca actataccac atagagcaca aattgccttt ttagtacctg gctcaaggta
     4441 gaataatcta ttgtggcaat gtggacatac accttcttct ccttgatatg aagctttttc
     4501 aaaatctttt gctgcttcaa ctatatttaa accaatttga tgaacttgaa caagccttgt
     4561 atcatccatt actaattcct ttgcccatgg gaaccaagca ttatctataa ctttccacat
     4621 tggtatgaga gctagcatag catgttcaca ttgaactctt gtaccccagt cagaacctcc
     4681 tattcccata aatgatatta ctttatcatt aagcattctt gaatctggaa tttttcctcc
     4741 tataacttct gcaacttttg ttcctataat gttatttcct ctatctgctc tgggtccaaa
     4801 acggtccatt atagtatgga ataatcctga tgccccttct tcaaaaatag ggtctgaaac
     4861 tactatacca tctgcatctt tcattttgtc aagcaaccat tcaaaatcat cttttaatac
     4921 acacatacta cctttcccag ataataaagt tttaacacac gcacaacatc cagtacaatg
     4981 tttaatgtta atagatgaca tttgaataaa ttctacttca gcccctgctt ctttggcacc
     5041 tttaagtgca actttacaca ttgtgtcatt atttccattt tttgttccaa aagaaatacc
     5101 tagtattttc attttcaact ctccctcttt agtatttatt taatcttctt tcatttttaa
     5161 tatatagaat ccatcatcta aagcatctat gatgatgtag cctctatcat caacgataag
     5221 atcttctgtt actgccaagc gtggtcctgg gaatcctggg aaataagatt cttctggtgt
     5281 tttatttgga tttggtggta taaaatatgc tagctctttg atataatatg ggtctgatac
     5341 atcataaacc cttagccctg catggaaata acagcaatat actctatctc ctctttgctc
     5401 taaccatggc ttattatcca ttggttcatg aagattatgt ggaccaaatg gccctggttt
     5461 tcctaatcct gcaacattaa agttaggata agggaaatct tttggaactt caggatatgg
     5521 aaattgagca attaatgttg gttttgttgg gtcactaaca tcaaccatat gtatattatt
     5581 catagcttga acatctgtaa tgttatctgg tttaaagaac tggaatcttt ctccctcatt
     5641 ttgaacaacg actaaatctc ttcctggcaa tggtaatgct gtatgagttc ttgcacctgc
     5701 aagtctacta gaaaatgcag gcgtaaatgg caattcacct aagcatcttg gtcttgttaa
     5761 atcttcaaca tctaatacaa ctaaaccagc tcctccataa ccacaatatg cttttccgtc
     5821 tcttacaaat ggaggtccat gaagccatcc tttatccata aattctgggc aatgaggtgc
     5881 tcctgcatca aaagttctat ttggatatcc atctgcatat tggtctggtc tccaccattt
     5941 acctatttcc actggattag taggatttat tatatctatg accctataaa tcagaccttc
     6001 aaagccaaca caatcacttg ataaatgtac atatcttcca ccattgtaca tgaatctatg
     6061 aacacccatt acatgcttta agccacaatc ccaatatcct aaaaacttag gatttaaagg
     6121 gtcttctttt aaactgtata ttctaattcc tgcttcacac ttaatatttg ctaatttagc
     6181 ttggtcaaca agtgctcctg gtccacttcc acaactcatt gctactatca ttaaatcatc
     6241 tgctatttga attttgggag ttgttgttgt aggatactct ttagggtcta acatttgaaa
     6301 atgttttata aattgtggat tataagggtc tgtaatatcg ctaatcatta ctccattttt
     6361 tttatttcct ccaaaacagc atccatataa atagtatttt tcatctcttt tgtacagagc
     6421 catttgaaac attccacaag ttccatctaa gttattaaag gaacaaactt caagattttt
     6481 aatatatccg ttatttccgt tgccttttgc ataaaattta tcttccattt tttctccctt
     6541 cttattttaa agtattatca attgatatac tttcatttta tgagacaaac ttacttaaat
     6601 ccaatatcgt tttcattgga atatataagt tattcttatt aataaattta taaaatctaa
     6661 tgtaaagata tacttaaaac atatattttt atacaaatat tttctaatat tctttaaaag
     6721 taaattttct tatactataa taagctttaa ttatagataa ataaggttta caaacaatca
     6781 agtttaaata ggggtgatta tttgaatact aaacaattag aatattttat atctgtagca
     6841 gaaaagctaa gttttaccaa aacagcagaa aaattttata tttctcaaac tgctgttaca
     6901 cagcaaataa aagctttaga agaacaaata aatgtaactc tatttacaag aagtaaacgt
     6961 catgttgaac taacaccagc tggaaaagtt tttttatctg aggcacgtac tataataaaa
     7021 aatatcaatg atgctattgc aaaaacacaa caatttgcac atggtttttt tggaactttg
     7081 agcattggaa ccttaatagg atatgaaaaa aacaaacttc aacaatattt aaaagaattt
     7141 tctaatacat accctaatat atctatggat atttgtacta atgaaataac cgaactttta
     7201 aacttagtaa aaaataattc tatggacctc gcttttgtta ttaatccaga aaatcaacca
     7261 ttaaaagatt ttgaatataa aactgtagaa agatattctt tggtagctct tttacctcct
     7321 ggtcatccac tttacaatga ggattctatc gatttaattg aattacaaca tgataaattt
     7381 atttttgtaa aggaaacagg ggatgagtat ggacaaaaat ctatgataca aaatagatat
     7441 cgtgaagcag ggtttgtacc taatgtggta caacgttcaa ataatcttaa tactattgaa
     7501 tcattggtat cttctaacat gggaatatcc attttacctt cattttgtgt tacagataca
     7561 atgatagaac gtgatattgc aatagtacct ataaaagaag tagaaaatag aattgaagtt
     7621 gtagttgtat ggaataaaaa aaatacaaat ccagcattag aaaagttcat ttctataata
     7681 taaaattaat caatattaac gataaaaata gcattaacca ttttttctat agttaatgct
     7741 attttctact attttattat ttaactttta atttaatatt tataatcatt aaaaataact
     7801 ctgtttaaca aatataagca agtcaaaaat gtgtctaaaa aagataaaaa ttttattata
     7861 tacaaatgta ttatctcaat atatctattt tttcattgtt catcagtcta agtacatgac
     7921 ctgtgcttac taaagaatta tccccataag ttgtatgagc taacaccgaa ctagcaaccc
     7981 caaactcaac tatatcttca ctcttcatat tttccaaata tgaatatata attcctgcta
     8041 catatccatc tccggcacca accctgtcat atatttctaa cttatactcc ctactttcaa
     8101 aataaccatc atcatttgat atatatccct ttataaatct attattcata tcatcaaatc
     8161 ttctcatagt tccagctaca atacctatat catactcttt tttaatcaat tctgaactag
     8221 attttatatt tagactatct atattaagta tcttatttat atctctataa ctagcaaaca
     8281 gcccatgaca atatggtagt attttcttat aatcatttac taaatcatca taagtccttt
     8341 ctggatttaa tgattgtcta aaattaaaat caaagaatac ttttatcccc ttttcataag
     8401 attttctagc tagttctaaa gcaatctctt taactgaatc tgtaagttgc atagcaatac
     8461 cacaaatatg gactatatcc attccttcta aacatttatc aatatcataa tcttgtaatt
     8521 tactctcaca aaatgaacta tttaacctat tcaaataagt cacttcagaa ggtctttctc
     8581 catacccatt ttcaagaaaa tacactccta tatgattccc tccatatgtg ataaagtcat
     8641 cttttactcc aagctttctt atatttgcac ttaatgcttt acctacatta ttattaggca
     8701 actttgtcaa catataggta ttgtatccaa agtttttaag tccagataat atatttaatc
     8761 ccgtgcctgt aattatataa tttagttcat tggtttgtgt taagaacttg tagtcaggta
     8821 ctgaaagtct catcattatt tcaccaaaag ctaatatatt cataaatcta tcccaataat
     8881 tctttcatta ttttataaag tactcctata tcttccactt tcgtttcacc tgtttcttta
     8941 tcaattattg atgtataaac atgaggtata actttttcta caccagcatc aagtgcaatt
     9001 tgaactatct ctttgaagtt atctaagtct atacctcctg ttggttctaa ataaaaatta
     9061 tgctttgcac aagcttttgc aacttctata tattcatcct ttgttttaag tcctcccatt
     9121 ggaaaaaact taattgagct tcctcccata tctttcatca tagctattgc tgtttctata
     9181 ggaactatac catctttttc ttttgaactt aatggacctg tggatatttt tacaaatcct
     9241 ggtttaccag ttggagaaac taatccattt attatactat cattttgacc aagtaatgct
     9301 ctagacgtag ccacacctgt aaacacttga tttatatgtt gtggctgaag ttctttagaa
     9361 atctgagata ccatatttga ctgatttggg tctccagctc caagccccac agatattgca
     9421 ttatctattt cctttgagta ttttttcata tcttcaatag ctgcttctac agtactgtaa
     9481 ttctttgata gtactcctat caatacatgc ccgtcacaag catcatatac ttctttagca
     9541 ttttttatgc ttcctgcaag aacatttaaa caaactctat ttttataata ttgtggtata
     9601 caattcatat ttatcccctc cattggtaaa atttaattta ttaaatcatt ataatgtcac
     9661 ttactctaca ctctaacttt ctactatatt ttttatccta ttataaatag tatctaaatc
     9721 acttccagtt acacttctta tatcaatctc aatcttgcct tcattagcct tatagtctct
     9781 agtgtatata gctatctctc cacttctaag ttcatttata atttgttgtg ttgacttatt
     9841 tattatatct tcattaaagt caatttctcc acgaagtatt tctctacctg ctgaatccct
     9901 agttatacta gcactcactc cctttatagt atttatcttg tctataaatg gatttagctt
     9961 gtcctccatt tcttttaaag ttactttatc attacttata tatctctcaa ttgcacatgt
    10021 tagacctaca atattttctt ttcctatttt catagctctt cctataccct ttgattgaag
    10081 ctttacatta tctatgtatt ttttcttacc tattactaac cctgagctag gaccctctat
    10141 agccttagca ccactgtata tcactaggtc tgctcccaat tcataatata catttaaatc
    10201 ttcttctgct gctgcatcta ctatcaaagg taaattatat tctttagcaa cttgtactac
    10261 atctaataca cttagcatac tcttttgaac acaatgatga gatttaatat acataattgc
    10321 tgctgtattt tcatttatct ctcttattat atgttccttg ttacacatat tagcataacc
    10381 ggcctcaaca acttctcctc ctccaagtct tatcattact tctactggaa ctccataatc
    10441 aacattatgt ccctttggaa ttattatttc ccttttaact tgtttcttag gattatttac
    10501 attgtaaact agactcatat catcttttgc tatcattgct cccacactct gtgcaatccc
    10561 agcagatgca ctagaaacta ttagtgcact ttcacaccct aataaatttg ctatatattc
    10621 acctgtttta tttataagtt cttctattat aaagaaattt ttacaccctt cacctattgc
    10681 ttcaataact tccttatcta ctgttgatac tcctagtata gtcattttgc ctgaagcatt
    10741 tataacacgt cttacagagt atttttcaaa tatactactc atacaatagt ttctcccttc
    10801 tactcattct cttctataga cttacctata tttaaatatt ctccattgac tactactgct
    10861 cttggcttta tagacgtact tgtgattact gacttattca aagaatcttg aagttctttt
    10921 tcttcattaa ccacatcaaa tatagttaaa tcagcatcat atccttcttt tattaaacct
    10981 ttatttttta aacttattgc atctgctgca tttttagtaa ctttatttat tatatcttca
    11041 agtgaatagc ccatatatat aaacttttcc attgttgtag atagattata cactggacca
    11101 tttattctat ttttaatata tatatctgta cttatagtgt ctggaaaaat tcctgcatcc
    11161 ttagatttca ttgcaatatc catactaaaa ctttcggttc catgacctac atcaaggatt
    11221 atacctcttt ctctagcttc tatgatttct tttttaacct cattgcctct aagtattcca
    11281 tttggctttc cattatatat atgggttaat atatctcctt tttccatata gtcaaatata
    11341 tcctctatcg ttggaggaga actcccaaag tgaaccatca taggtagatt aagttcattt
    11401 tttattcttt tagctacttt taaaggttca acatcatttg aaattacaac acttttactc
    11461 attctagctt tgattccaac aacaaaatct ttgtattttt taactgctct tttaagttca
    11521 tattcatcaa tattcatcat acttgaaagt tcatcttgag atgttattcc ctgcttagca
    11581 atatttatta aagcatatac atttgtttta tattttttag tatcttcata aaatttacct
    11641 atatctaaag ctcctgttgt tcctgcatct actacagtag taactccact ttttattcca
    11701 acctcatctg gataatcctt ataaaggtct agtctttcgt agcaatgcgt atgtatatct
    11761 atccaaccag cactaataaa tttctctcca tctaagttta taatttgttt tgcctgatac
    11821 ttagaatcat ctatctgatt ttttacttct attattttat cgtctaatat agctatatca
    11881 attatacttt tatctactgt ttttccacat tttattaata tgtctatctt cactttactc
    11941 tccctctaaa ataatcttaa atttactcca aggttcaata aagtctaaaa gtattatttc
    12001 gtcttccttt attctaccca ccttattttt tctttcatct gtacaatctt gaagtatcaa
    12061 ctgtaattcc cctttatact ttccaaagtc attattacca atcactacat ctcctctttt
    12121 aaagaggttc ttattatcat gtttagggaa attttcttta gcatattttt ttcttacttc
    12181 tgtagatctt gctacctgat tagttatatc tcctcttcta aagtgttgct catttaccat
    12241 tattatcttt tctacttcag aagttttttc ttcagctact acattaaata caacttggta
    12301 ttcatttact tcagacattc tttttaattc atcttcactt gcatatgcat tccctacaat
    12361 tacatcatca atcaatcctg ttgcgaataa gtgcttagtt gctgtagcaa tatctaagtt
    12421 tctatgcatt tcaagcgtac acagcccatc atttatattc caaggcccta ttgttgcgta
    12481 ttttgaatta acaaaagcag cagttcttat tccatacttt ttaaatctat ttgaacatct
    12541 tacaaaaaaa tcatatggca agcctgtacc cctttgagga taaaaattgt gacaaccata
    12601 tatattattt acatttggct tgtaagtaat tatattatcc aagtatgcta catcattact
    12661 catatttagt tccactttta aatcatatgg attgaaagaa atcatagctt ctttacttcc
    12721 atcatatcca tagtcaagcc ttattccact agcatttaac tcactaaaaa atgttaaatc
    12781 atcataagat atttgtaata tatcaaatat gtttggagct atatcaagga ttacttcaaa
    12841 tcctaattct cttgcatagg ttataatttc tttaaaatgt tttttaactt cttccttagg
    12901 tcttgtaatt gaaagcatgc acataaatat tcttttaaat ccgtattttg aagccatttt
    12961 tatatatgcc ttatcatctt ccatgttact atgctctgga tatactgata ttcctaatct
    13021 tctttccatc tttctcctcc tcaaattaca ttatttaaaa tatataaaat taaaggcaaa
    13081 ggtcattcct cctaccttca cccttaataa cttataagct ttttctatat tttatatagt
    13141 acaaaacctc tatatctatg tatttttata aattttcact catttcttct tgttctcttt
    13201 gtaattgttg tttttcaaaa actttgaaga atggataata aacagcaaat gatatcacaa
    13261 agttgattaa tactaagata cctgctagta tactccagtt agtactcatt actgctgcta
    13321 ttggtgctgg cattgcaaat ggtaatcttg ctaccatcat aggtataacc cctgtaatag
    13381 taagaacata tgatagaatt gtcattatta taggtccaat tataaatggt atccctagta
    13441 ttggattcat aactattggt gctccaaata ttataggttc atttatgttg aataaccctg
    13501 gtaaaaatcc taatctacct aaacttttta gatattgtga cttagaaaac ataaacatta
    13561 caactaatgc aagagtagcc cctgctcccc ctatccatac ataccactgt aaaaattgtt
    13621 ctgtaaatat gtttggcatt gcatgagctg atactccttc tgtgaatgca gttatatttt
    13681 ctgctataga catgtcccat ataggtctta ttactggccc cattatagct ggaccatgta
    13741 ttccaagtac ccagaataaa gttattagga atactgtcag taacccacca cctaaactat
    13801 ttcctgataa gaatcctttt aaaggtgata acatagcgct tagtgcaaca cttatatcaa
    13861 atcctaaaat atgtcttatt acccagaata acattaaaat tactgctgtc ggtaataatg
    13921 cagtaaatga atttgaaacc tctactggaa ctcctgctgg catcttaatt gtaatattct
    13981 tttccttcat aaaacgatat atttcaactg taattaacga tgttacgata gctccaaata
    14041 atgatggcga attaagtttt gccatatcca tccatctacc agcttctata actccaccta
    14101 ctggttcaac tacttgagtt ggaactattg tagaaactaa aaatgccata actgcaagca
    14161 taccgacacc tgttgtatct aaatcgtagc ttttagcaag tgatgatgct atcccaaaag
    14221 tagcatatag agataaaaat cctacagtat atctaaatgg tatatctaat attgctttaa
    14281 atggagctat aaaatccata taagcatcaa taggtatgtt taataatatt gtgaaaaatg
    14341 accccactat tgttagtggt aatgttgcta ttatacctgc tcttacagct ctcatatgtc
    14401 tctgattact tatcttcgct gctacaggca taatcttgcg ctctaaaaaa cttgcgaatt
    14461 tttccatagt tatccccctg tattattgat attatcattt atttataatg caataacgat
    14521 ttcataacat aaactaattt ttatgtaatt gataatatca cttatttatt aaaaatatta
    14581 taacattata atgtttggtt gtaaacatta taatgttata atattttttt gtttttacaa
    14641 ctaaacaaaa atcctgagca tattatcatt tcaataaact caggattctt attatatttc
    14701 gtatttaatt acatagtttt gtacttctgt attatatctt gcaaatgata gctcaactat
    14761 attatcattc tcatcatagg tctttctttt tcttcctaat aatatgtctt tagctagatt
    14821 tagagccttt gatacctcta gttctgattc ttctataaaa aattcatctt caaatctagt
    14881 tatatttata tttctcctat ataagtacat atataaagaa ctttcattat taaaactttc
    14941 aacagttaaa ttttcatcaa aaggaatata atgagtaaaa aatatatatg gcttactatc
    15001 taagtaatac attctagtaa tcttgctaca tttttctcca aatatactaa atacttgtga
    15061 atttgtatca tttattattt tttcaattga aatagtcttt ttagataact tatgaccttt
    15121 agataaaagt atacttgaaa aagactctgc ttttgatagc ttattaaaaa gactattact
    15181 tattactttt gtcccttttc cacttttttt ctctacatac ccttctgatt ctaatatctc
    15241 aatagctttt ctaatagtaa ttttactgac tccaaactct tgctctaact ccctttctgt
    15301 tggaatattt tcatcaattt tatattcgtt atttaatatt ttttgcttta atatatctac
    15361 aacttgtata tataatggtt gatttcttaa catgattcac ctc
//
LOCUS       DAEOSP010000086        15307 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.086, whole genome shotgun sequence.
ACCESSION   DAEOSP010000086 DAEOSP010000000
VERSION     DAEOSP010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15307
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.086"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            53..1612
                     /locus_tag="KRH54_002068"
     CDS             53..1612
                     /locus_tag="KRH54_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG8579725.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            1718..2677
                     /locus_tag="KRH54_002069"
     CDS             1718..2677
                     /locus_tag="KRH54_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8579726.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            2680..3492
                     /locus_tag="KRH54_002070"
     CDS             2680..3492
                     /locus_tag="KRH54_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8579727.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIITSFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(3708..4610)
                     /locus_tag="KRH54_002071"
     CDS             complement(3708..4610)
                     /locus_tag="KRH54_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG8579728.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            4965..6143
                     /locus_tag="KRH54_002072"
     CDS             4965..6143
                     /locus_tag="KRH54_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG8579729.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVRLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDILIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            6152..6808
                     /locus_tag="KRH54_002073"
     CDS             6152..6808
                     /locus_tag="KRH54_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBG8579730.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            6808..7473
                     /locus_tag="KRH54_002074"
     CDS             6808..7473
                     /locus_tag="KRH54_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBG8579731.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKKINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            7578..8354
                     /locus_tag="KRH54_002075"
     CDS             7578..8354
                     /locus_tag="KRH54_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG8579732.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQESLNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            8416..9750
                     /locus_tag="KRH54_002076"
     CDS             8416..9750
                     /locus_tag="KRH54_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG8579733.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            10247..11173
                     /locus_tag="KRH54_002077"
     CDS             10247..11173
                     /locus_tag="KRH54_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBG8579734.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPDNNKPESNKPEDN
                     NNSDSTNKPDDNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKK
                     SQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGH
                     RKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(11566..15105)
                     /gene="nifJ"
                     /locus_tag="KRH54_002078"
     CDS             complement(11566..15105)
                     /gene="nifJ"
                     /locus_tag="KRH54_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBG8579735.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHACIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRIQV
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKVDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVLEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         5404 a         2327 c         2359 g         5217 t
ORIGIN      
        1 atacaataaa aaatatgtgt taaatattat tttatggagg ttttataaga atatgaaatt
       61 taaaaaattg gcatcattaa tcttagtttc atcattaatg cttacattta cagcctgtgc
      121 ttccaatgat aaagacaagt ctagtggaag caaatctggt ggaactgttg taatacctat
      181 ggcaagtgac ccagatataa tcaatggagc ttttgcaaac gttaaagaag actctcttgc
      241 atcaaatata gtttatgctc ctctttatac ttatgaaaaa ggaaatttag taaattattt
      301 agcagaaaaa gtagatttta aagatagtaa ggaattaact ataaaattaa aatccaattt
      361 aaaatggcat gatggaaagc caataactgc tgaagatgtc ttatttacat ttaatacagt
      421 tttagacgaa aaacaaaatt caccatcaag acaatactta ttggtgggtg agaaaccagt
      481 taaagtagaa aaaattgatg atttaacagt taagattact ttaccaactg caagtgaatc
      541 tttcttatat ggtatatcta aaatttctcc aataccaaaa catgtgtttg aaggagaatc
      601 aaatatagct aagtcagaaa aaaataataa tccagttggt tctggtgcat tcaagtttaa
      661 ggaatggaaa aaaggtgaaa gcatagtatt tgaaaagaat gcagattatt ttggtggaga
      721 acctaaggca gattctatag cgctaaaaat aattcctaat gaagcatctc aagaagcagc
      781 tttaaataat ggcgaaattt cacttatgaa gacatctgct gaaggatatg aaaaggcaaa
      841 atcaaactca aacttacaaa cttatactta tagtgaagaa agattaaact acattgtatt
      901 taaccaaaat atatcaaaca tggctaacaa agaagtaaga caagccttat catatgcctt
      961 aaatagaaat gaaatgatag aatcagcata tggaaaagaa ggttctgtac ctgctaaatc
     1021 tatattagtt ccagaagcag acttctatac agaagaaggt gttgaaggat atgaccaaga
     1081 tacaaataag gctaaagatt tattggataa aagtggtgtt aaaattgata aattaaaaat
     1141 aggttataac acaggtagat ttgggcataa aaactatgcc ttagtagctc aacaagagtt
     1201 gaaaaaaatt ggtatagaag ctgaaatagt tccttatgaa agtaaggcat tcttcaatat
     1261 actatttagt aatagcactg aatgtgatat gtatgtaaat ggttatgctt ggggattaga
     1321 accaaaccca tatagaggta tgtttgaaac tggtcaatat tgcaaccaaa ctaaatattc
     1381 taatgctgaa atagatgctt tatgggaaaa aggatttact gagttaaaca aggaaaaacg
     1441 tgaagaaata tataagcaaa tacaacagga tatatctaag gatgcgccta tttacactat
     1501 agattatgaa caaaatttaa tggcagctca aaaaaatcta aaaggaataa aagatgctaa
     1561 accatctcct gcaatattat ttgaagattg gtctaaactg tatgtagaat aattgatgta
     1621 aaattttata ttattaatta gaaaaggaga tgtctaaaaa taatattgtt ttattatttt
     1681 aaaacgagac atctctttta attaggagaa tttacttatg ttaaaattaa tattgaatag
     1741 agtaaaagtt ttaataccta tgttaatctt aatatctata ttatcatttg gacttttaga
     1801 attagctcct ggtgacccag cagatgcata tataaatcct ttaatgtctt cacaggatat
     1861 tgaaaatata agggttaata tggggcttga taaaccagta tatataagat atttaaactg
     1921 gttgaaaaac actttaagtg gaaacttagg tatttcttat ataaaccata tgcccgttac
     1981 agaacaaatt atggaaaaaa tgggaaatac atttattctt atgggtactt ctttgatatt
     2041 ttcaatattg gtagctatac ctcttggaat atttttagct gtaaataaaa attcaataac
     2101 tagtaaagta tcatctgttt ttaattatat aggtgtttct ataccttctt tttggatagg
     2161 tatgatactc atttcaattt tttcagttaa acttaacatt ttcccatcag gaggtatgca
     2221 tactattgga aatgatagta ttggagattt agtaaaacat ttagttttgc ctgtaataac
     2281 tttaggtcta tataatactg ctatttttac aaattatgta gaagctagtg taaatgaaca
     2341 attaaaaaaa caatatgtta ttacagcaag agcaaaagga ttatcagaaa aagtcatact
     2401 atttaaacat gtcttgaaaa attcactgac atcattggtt acaatcctag gcatgagtat
     2461 acaaaaatta gtcactggtg catttgttac agaagtagtt ttctcttggc caggtatggg
     2521 aagattaatg attgattcaa ttttttcaag agattatact gtaattatgg caataactat
     2581 gttatcagca ctatttttaa ttttaggtaa tttagttgct gatatactat acttattaat
     2641 tgaccctaaa ataaaatcta gcaaaggagg attctagttg tgtttacaag tatcttaaaa
     2701 aaattaaaaa aagataaatc ctttgctttt gcatgtatta ctcttagcat aataatcata
     2761 ttaattataa cttcttttat attaaatata gactctaatg caattgatac tacttctaaa
     2821 cttcaagaac ctagcttaaa gcatatcttt ggtacagacg aacttggtag agattacttc
     2881 tctagagctc tttatggtgg aaggatttca cttagtgttg gtattttatc aatgcttatt
     2941 tctgtagtat ttggtactgc tgtaggtgtt attagtggat atattggagg taagctagac
     3001 caattcttga tgagattagt ggatatgctt atgagtatac caagtttctt agttttgatt
     3061 gtattaaata cttatctaac acctaaagtt tccactatta tactaattat tggattctta
     3121 agttggatgg aagtttcaag aattgtacgt ggcgaaacat taaaaataaa ggaaaatgaa
     3181 tactgtctag ctgctaaagc tactggtatc aaaactagaa atatactatt aagacatata
     3241 gttttaaact taaaagagac tattgtagtt gctggttcgt taaatattgc taatgctatc
     3301 ttaactgaat ctgctcttag ttttttaggt cttggaatcc aattgcctct tgcatcatgg
     3361 ggtaatatgc ttcaagatgc tcaaagacat atttttgaca agttatattt agctgtattt
     3421 ccaggattac ttatattttt aacagtcatg agtttgacta taataagcaa aaaattttct
     3481 agtaatactt aacaaatttt atatagaaaa taaaaacagt gattatctaa agatatacac
     3541 tgtttttatt tttcgtttaa aaaaatacat attttgtata ttataacttt aaatactact
     3601 ctaccatgtt atgtaaagcc tattattgct ctcaaacata tagatgaaaa actaatagtt
     3661 tattataata aaaattgatt tatttatttg ttattttaat aacattttta agagttcttt
     3721 ttactaaaga aatctataat aaatttaaat acatgttggt ctacatggct ataacgttct
     3781 tttttaggtc tacatagtat aactttccat gtaataggat gattaaaagg cttttctata
     3841 attttgctat tttcaaaaaa atcatgaact ggtgatggta gaattgttat aaaatcagaa
     3901 tttgtggttg atagcaacaa aaaatcccat gaaccagaca ttatatatct ttttaactta
     3961 acgttttcat cagtaaattt ttgcattagc ttatgatgta tcataaaagt aggattaaat
     4021 attgcaagta actgctcatt caaatcgttc caatctatct tatccttctt agcaagtgga
     4081 tgatttatat ttaaaaatgc tgtaagttca tcttcttgaa gcacatgttc agttactaca
     4141 cttttatcaa ttttatgtgg atgtaacaat actgcataat ctaattcctg taagataagc
     4201 attctattta agtcatatgc acccttctct actatatcaa attctatatc tggattattt
     4261 gcaactaact gtgcaactac atctgaaaat gctattccaa gtataagtgg aggtattcct
     4321 attctaactt ttcctttaaa ttgaactgaa tcttctctta aatcttccat catatttttg
     4381 tattcattta tgatatgttc tgcatttaca taaaatcttt ctcctgctgg tgttaaccca
     4441 tttaacctgc ctttatacct ttcaaataaa tatacatctt cactttcttc aaaatttttt
     4501 ataatttgac ttaatgctgg ttgagatatg tgaagtgtct tagatgctat tgataaatta
     4561 aaaccagatt tcactatttc aacaaaatat ttcaactgtt ttatatccat tttaaacccc
     4621 cttccccaaa agtataattt agattatatt ttttcttcta atgatatttt ataggaaaat
     4681 caacataatt tcattaatat tttaacaaaa tatttttcta aatctactat aaatttttat
     4741 cctataaatt tttcttatac actatataaa aaattagact tagacaaaac gcttttttcg
     4801 ccgtaatcta taattagaaa caggaaaacc taaagcatta ggaataaaag taattgaaat
     4861 acttaaaaac aagcttgtat taaaaaagta ctttattata gtgttcccta atttctttat
     4921 tcagaaaatt tttaattatc attcaagaaa ggtaactatt agatatgaaa gatgtagtaa
     4981 tcgtatcagc agtcagaaca cctataggaa gttttggagg tgtatttaaa aatacttcag
     5041 ctgttcaact tggaactatc gctgttaaag aagcgatatc acgtgtaaga cttaatttat
     5101 ctgaaataga tgaagtaata attggaaatg ttcttcaaac agggcttgga cagaatgttg
     5161 ctagacaaat tgctataaat gcaggtatac caaattctgt tccaagctac acagtaaata
     5221 aattatgtgg ttcagggctt aaaagtgtcc agctagctgc ccaatcaatc acatctggtg
     5281 aaaatgatgt ggtaattgca ggtggtactg aaaatatgag ccaagctcct tatatcgttc
     5341 caactgctcg ctttggtagt aaaatgggtg acataaccat ggtagatagt atgctaacag
     5401 atggccttat tgatgctttc aatcagtatc atatgggaat aactgcagaa aatattgcaa
     5461 ctaaatttga atttactaga gaaatgcaag ataaattggc attagaaagt caaaataagg
     5521 ctgagaatgc tataaaaaac aaccgcttta aagaagaaat cgttccagta gatattttaa
     5581 tacgtcgagg aaaaatagaa actattgata aagatgaata tcctaaatta ggtatgacat
     5641 ttgaaggttt agctaaactt aaacctgcat tcaaaaaaga tggtactgta acagctggaa
     5701 atgcctcagg tattaatgat ggtgcggcta tgttaatact aatgagccaa caaaaagcag
     5761 acgaactagg tattagacca cttgctaaaa taaaatccta tgcttcagct ggtgttgaac
     5821 ctgaagtcat gggtactggt ccaatccctg caactagaaa agcactgaaa aaagctggac
     5881 ttagcatcaa tgatatagac ttaatagaag caaacgaagc gtttgctgcc caagcattag
     5941 ctgttaaaaa tgaacttcaa atagattcat caaagttaaa tgttaatggt ggtgcaatag
     6001 cattaggaca tccaatagga gcaagtggag ctagaatact tgttacctta atttatgaaa
     6061 tgcaaaaacg taaagttgaa actggacttg caacactttg tattggtggt ggtcaaggaa
     6121 tatcaatggt ggtttcacga taggaggaaa tatgaataaa atagttagca ttgatgaagc
     6181 tctatctcat gtaaaagatg gagatacaat aatggttggt ggattcatgg ccaatggttc
     6241 accagaaaat ttaattgatt atttatgtag taaaaatata aaagatttga cactaatttg
     6301 caatgatact ggctttattg acaaaggagt tggcaaaatg gttgtaaata agcaatttaa
     6361 aaagataatt gcttctcatg taggattaaa taaagaaact ggaagacaaa tgaacaacaa
     6421 tgaaacagat gttgagttag ttcctcaggg aactcttgct gaacaaatac gtgcagctgg
     6481 ttatggtcta ggaggtatac tcacaccgac tggtattggt actcttgttg aggagggaaa
     6541 gcaaaaaatt gttgtcgata gcaaagaata ccttcttgaa aaaccaattt atgcagatgt
     6601 tgctcttcta tttgcatcaa aggtcgataa agcaggtaat ctagtttata aaggctctat
     6661 gaacaatttt aataatttaa tggcatctgc tgccaaaatt acaatagttg aagcagatga
     6721 aatcgttgaa attggtgata ttgacccaaa tgaagttaat acaccaggta tttttgtgaa
     6781 ttacatcgtt gaaggaggta atttataatg gataaattag aaatgcaaga atatattgca
     6841 aatagagtat ctaaagaatt aaaagacgga gccgttgtaa accttggaat aggtctacca
     6901 accaaagtag caaattatat tccggataat gtaaacgtta tcttacaatc tgaaaatgga
     6961 tttttaggtt taggttcagc cgaagatgga aattcaagtg atgaaactat tgtaaatgca
     7021 ggtggtcaac cagttactat acttcctgga ggatgtttct ttgatagtgc tacttctttt
     7081 ggaataatca gaggaggtca tgttgatata actgttcttg gcgcacttca ggttgacaaa
     7141 tatggcaata tagcaaacta tatgattcca ggtaagatgg ttccaggtat gggtggagct
     7201 atggacctag taactggagc taaaaaagtt attgtagcaa tggaacatac ttcaaaaggt
     7261 tctgcaaaaa tattaaataa ttgtactctt ccacttactg caacaaatgc tgtagatttg
     7321 atagttactg aaatgggagt tatggaagta acatcagatg gaattttact taaaaaaata
     7381 aatccagctt tcacattaga tgatgttatt tctgctactg aagctccttt aatattgtca
     7441 gattcattaa atggaaaaat atctgttgtt taaaataatt aaaactagaa aataaaaatc
     7501 tcttagacgt attacatata aatttgtatt gcttaaaata ttaatataaa aataaaataa
     7561 aattggagga aatgaaaatg gttaaagata aagttgtatt cgtaacagga gctgcaagtg
     7621 gaataggtaa acaaatagct gaatcgttct taaaaaatgg tgctaaagta atgttctctg
     7681 atataaatca agaatcttta aataatacca taacagagtt acaaaaaaaa ggttatgatt
     7741 gcatgagtgt aaagtgtgat gtaactaagg aagaagaaat taatcacgct atagacaaaa
     7801 ctgttgaaaa gtatggcaga ttagatatat taataaacaa tgctggactt caacatgttt
     7861 ctatgataga agatttccca actgataaat ttgaatttat gataaaaata atgctgacag
     7921 caccttttat agctactaaa aaagcatttc ctataatgaa aaaacaaggc tttggtcgta
     7981 taataaatat ggcctcaatc aatggtgtaa taggatttgc tggaaaggct gcttataact
     8041 cagcaaaaca tggacttatt ggactaacaa aagttgctgc tcttgaagct gccgattctg
     8101 gtataacagt taatgcttta tgccctggat atgtagatac tccacttgtc agaggtcagt
     8161 tcaatgacct ttcaaaaact cgtaatatac cattggaaaa tgtcttagag gaagttttat
     8221 acccacttgt accacaaaaa cgcctattag atgttcaaga aattgccgac tatgctatgt
     8281 tcttagcaag tgataatgct aagggtatta caggacaagc ttgcttacta gatggaggat
     8341 atactgcaca atagttatta tatttagggc ttgaagaaag tattcttcag gtccttctac
     8401 taaggaggga tatttatgag tattatcgca ccaattggta taattattgg tatcatagct
     8461 attatatatt tttcagttaa agaaattcat ataactatag ctgctccaat agcaacttta
     8521 atagttgtta ttttaaataa aatggatgtt gtaacgtcaa tgctaggtgc tgacaaaaac
     8581 aattatatgg gtgcacttgg aaactatata atgagttatt ttgcaatatt cttattaggc
     8641 tcaatacttg ctaaatttat ggaagaaagt ggagctactg tatcaatagc tgattttatt
     8701 ttaaataaat ttggctctaa tcatccttat agagttttag ttgctatatt tatagtaagt
     8761 tttatattaa cgtatggtgg tataagccta tttgtagtta tgtttgcggt cattccactt
     8821 gcaagaactc tatttaaaaa acttgatatt tcttggaacc ttattcagat accactttgg
     8881 cttggtattg caacaattac tatgactata attcctggaa caccagccat tcaaaatgtt
     8941 atacctattc aatatctaaa tacgtctctt actgcagcta ctataccaag tattgtaggt
     9001 agtataggtt gtgctacatt tggacttgtc tatatgaaat atgctctaaa caaaagtatt
     9061 agaaaaggtg aaacttatgc aacttacact tcagaattag aagaagaatc tttagataga
     9121 gaattaccag gattttttgc aagtattttt cctctattag cactcgtaat tatagctata
     9181 actggaagta cttttggttc agaatttatg aaaaaaaatg taatttacat agctttaata
     9241 gttgcaattc ttttatctgc ttttttattc cataaatttc tatcatctaa gatacagaca
     9301 ttgagcatag gtgctagtgg ttctataggt cctatatttg ctacatcttc tgcagttgca
     9361 tttggagcag taataatggc tgcacctggt tttgatgtat ttagcaaact tataatgtca
     9421 ataccaggta gtccacttat aagtcttact gttttgactt caacaatgtc agcaataaca
     9481 gggtcctctt ctggtgcatt aggaatagta atgccaaact ttgcagatta ttatttaagt
     9541 actggtctaa atccagaatt aattcaccgt gttgctacaa tagcctcaaa tatactaact
     9601 atagttccac aaagtggtgt ttttcttaca ttcttaagct tatctaagtt aacacataaa
     9661 actggattta aagaaacttt cattgtagtt gcagttggtt cactaatagc agaaattatt
     9721 gtaattacat taggattact aatgtattaa tataaaacaa attaaaattc ataatatgaa
     9781 aggtggtgtc tcaaagtgaa ctttttactt aatgagacac tctttttata tgaaataaca
     9841 taagcctatc atttcaacaa taaaaaagaa ccttatctct attttgagac agaccctttt
     9901 tatttcttaa agatttaaat aattataatt tgttgctaat acgaaagcat ctactgaata
     9961 aatttaacca aataaaaaaa aatttatcat aactataaat ttacgttatg gaattattat
    10021 aaaaatatta ttttctctaa ctaagtaatt ccatactata caggctttta atacaaattg
    10081 tcaaacacac tgaaatatac ctatttattc catatatatc ttttttacat atctttacag
    10141 taaaaaaagt cttaatctta tgttagtata gattcacggg taacccccta cactaaaggt
    10201 tacaaatcag taacattttt agaattttta gattaagagg tgtttaatga tgagaagaaa
    10261 tataccaaca aaattaaaaa ctatattagc tttaggagtg ttaactttag caacagcttc
    10321 aagtgctgtt actgcaagtg cttcatcact tgaaaataat gtaaatataa gtaacaaaag
    10381 tttagtatat agtaatacta aatacaagtt ttctgcaaaa ccaaattgtt ctgtaaaacc
    10441 agaacttaag cctgataaag ataactcttg taaggataag aatcataatg acaagaattg
    10501 caacaacact aataaacctg aggacaacaa taattcagac tctactaata agcctgataa
    10561 caataaacct gagtctaata aacctgaaga caacaataat tcaggctcta ctaataaacc
    10621 tgataataat aaacctgagt ctaataaacc tgaagacaac aataattcag gctctactga
    10681 taaacctgat aacaataaac ctgagtctaa taaacctgaa gacaacaata attcagactc
    10741 tactaacaaa cctgatgata ataacaactc aggctctaca agtgaaagtt tttcagctta
    10801 ccaaaaagaa gttgttgact tagtaaatat agaaagagct aaagctggat taaatcctct
    10861 tactcttgat tcttctatat caaatgttgc tacaaaaaaa tctcaagata tgattgataa
    10921 caactatttt tcacacaatt ctccaactta tggttctcca tttgacatgt tgaaaaaatt
    10981 tggaataagc tataagacag caggtgaaaa tatagctatg ggtcaaaaaa ctcctaaaga
    11041 agttgtaaac gcatggatga attctgaagg tcacagaaaa aatataatga atccaaattt
    11101 cagtaaaata ggtgttggag tagcacaaaa aagtggtggt tcaatttatt ggacacaaat
    11161 ctttgtagga taaaatactc tttttccata catatcaaaa gagtatctct aagtgataaa
    11221 aaacacaaat gagatactct tttgtatcta ttttaaaatt atcaaatcca aatatacata
    11281 ctataaatat tatttgttct cttaatttta gtgtgtagag caatatataa aaagtattgg
    11341 caagatgaaa atggaaaact taaaaaagat atttaatagg taaaaacaaa atcttaatat
    11401 cttcataaaa aaaggctgtc tcaaaataga gataaggcct ttttcattgt tgaaatgatt
    11461 aaaatatatt tgatttcata caaaaagagt gcctcatgaa ctaaaaagct cactttgaga
    11521 cactctcttt tatcaattct atataagcat tataatactt taatattatt cagctaattt
    11581 tttgtatcct tcatatcttt ctttagcatc agcttcagct ttagcaaata atccatcagc
    11641 ttcctctggg aattgtttag ctatagcaga atatcttact tgtgcaagta tgaaatcttt
    11701 gaagcttgca gttggttctt ttgaatctaa agtgaatggg tttttacctt cagcttttac
    11761 ttctggattg aatctgtata aatgccagta tccgcaagca acagcttgtg cttcattagc
    11821 tacagttctt cccatacctt cttttaatcc atggtttata catggagcat aagctattat
    11881 tagagatggc ccatcatgtt tttcagcttc taaaacggct ttgataaatt gatttttatc
    11941 agcacccata gcaacttgag ctacatatac gtttccatat gtcatagcca tcattcctaa
    12001 atctttcttt ctagattttt tacctgaagc agcaaactta gccatagcag cagctggagt
    12061 agctttagaa gcttgacctc ctgtatttga atatacttct gtatcaaata caagtacgtt
    12121 tacatcttca ccagatgcaa gaacatggtc taatccacca taaccgatat catatgccca
    12181 accgtctcca cctagtatcc attgagatct cttaactaga taatcttttc tatcttttat
    12241 agcttttact aattctttag ctttttcatc tgtacaatca caagctggtt tttctaataa
    12301 ttcaacaacc ttagcacttg ctactttaga agcttctcca tcatttctag aatctaacca
    12361 ctctgtaaat actgctttag catcttcaca gatatccata gaaactaact ctgtcatagc
    12421 atctactatt ttatttctta tttgcttaac agctgtgtac ataccaaatc catattcagc
    12481 attatcctcg aataaagaat ttgcccaaga tggaccttta ccttcatggt tacaagtata
    12541 aggaattgat ggtgcagaag ctccccatat agaagaacat ccagtagcat tagctatcat
    12601 cattctatct ccatataatt gtgtaactac tttaatgtac gcagtttctc cacatccagc
    12661 acatgctcct gaaaactcca ttaatggttg tctaaattga cttcctttaa cagtatttgc
    12721 aggcataaca tcaacttttg gagctacttc atcaggattt actgcaaatg cccaattatc
    12781 taactctgta tcaagttgag tatctattgg cttcataact aaagcttttt ctttagctgg
    12841 acatatgtca gcacagtttc cacatcctgt acagtcaagt ggactaactt gaattctata
    12901 ttttaatcct tctaatcctt tacctaatgc ttttttagct tcaaatcctt ctggagcatt
    12961 agctaattct tcttcagtaa ctaatactgg tcttatacat gcatgaggac atataaaaga
    13021 acattggtta cattgaatac agttatctat tatccattca ggaacatcaa cagctatacc
    13081 acgtttttca taagctgcag taccacaagg gaatgttcca tcttctatac cattgaatgt
    13141 acttactggt aagttgtttc cttcttgtgc agtcattggt cttagtatat tttttataaa
    13201 ttctggttct ttaacttctc ctttatctgc atcaacagca ttttcccaag aagctggaac
    13261 ttcaacttta actaaagcat ttattcctgc atcaacagca gcatagttca tattaactat
    13321 tttttcacct tttttaccat aagctttaac tatagaatct tttaagtatt taacagcatc
    13381 ttcttctggt attatttcag ctaatttaaa gaatgcagat tgcattatca tgtttattct
    13441 gtttccaaga cctatttctt gtcctagttt aacagcgtta actgtataga attttatatc
    13501 atttttagct atatattgtt tcatatgagc tggtaaatgt tcttcaagtc ctgcttggtc
    13561 ccatatagtg ttaagtacaa atgtaccacc ttttttaaga ccttttaata aatcatattg
    13621 ataaacatat gattgtttat gacaggcaat ataatcagct tcatctatta aataagtagc
    13681 tcttattgga gtatcaccaa atcttaagtg agacatagtt ataccaccag attttttaga
    13741 gtcataatca aagtaagctt gagcatattt ttcagtatgg tcaccgatta ttttgatagc
    13801 ttgtttatta gctccaacag taccgtcaga acctaatccc cagaacttac atcttatagt
    13861 tcctttagaa gctattttta ttggctcaga tggagttaaa gaagtattag ttacatcatc
    13921 aactatacct actgtaaatc catttttagg ttgttctgaa actaagttat caaaaacagt
    13981 ttttatatct gaaggagtag tatcttttga acctaatccg tatcttccac ctattatcat
    14041 aggagcattt tcttttccat agaatacatc acgtacatct aagtataatg gttcaccagt
    14101 tgaacctggc tctttagttc tatcaagtac acatattctt tcaacagtag atggcatagc
    14161 atctaagaaa tgtttcattg agaatggtct atataaatga actttaacta aaccatattt
    14221 ttctccctta gcatttaagt aatctatagt ttcttctatt gtttctgtta ctgaacccat
    14281 agctattaat acgtatttag catcttctgc tccatagtaa tcaaataaac tatgttttct
    14341 acctgttaaa ttactcattt tttccatgta tttttcaact atacctacta tattttgata
    14401 gtatttatta gaagcttctc tagtttggaa atatatatct gggttttgag cagtaccacg
    14461 agcaactgga tgatttggag ataaagcatt atctctaaat gcttgaactg cttcaaagtt
    14521 taataaactt gcataatctt cattttctag taattcaact ttttggattt catgtgaagt
    14581 tctgaatcca tcaaagaaat gtataaatgg taatctacct tctattgcag ctaaatgtgc
    14641 aacaggtgct atatcagcta cttcttgaac tgaaccagaa gctaataata cacatccagt
    14701 ttgtctagca gccataacgt cttggtgatc cccaaatatt gataacgctt gagatgctaa
    14761 agcacgagca cttacatgga atactcctgg taataactct cctgcaactt tatacatgtt
    14821 aggtatcatt aataataaac cttgagatgc tgtaaaagta gaagttaaag ctcctccttg
    14881 taaagaaccg tggaatgttc ctgatgcacc tgcttctgac tgcatttcaa caacattaac
    14941 tgtttgtcca aatatatttt ttcttccttg tgatgcccac tcatcaacta cttcagccat
    15001 agttgaagat ggtgtgattg gatatatagc tgctacatct gtaaacgcat aggcaacatg
    15061 agctgcagct gtatttccat caagtgtttt cataaactta gccataatat gtatcctcct
    15121 tgatagctac aattgtactt gtttataaca agtctatttt tttatattta catataattt
    15181 acacaacaaa aatataaaca cacatatagt cgcgcaaata ctctatcttc taatataata
    15241 ctacagatta gataaaattc ctttgaaaat tgtaaattaa ttacatattt aagtagattt
    15301 tttttaa
//
LOCUS       DAEOSP010000087        15182 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.087, whole genome shotgun sequence.
ACCESSION   DAEOSP010000087 DAEOSP010000000
VERSION     DAEOSP010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15182
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.087"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            54..977
                     /locus_tag="KRH54_002079"
     CDS             54..977
                     /locus_tag="KRH54_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG8579736.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(1149..2291)
                     /locus_tag="KRH54_002080"
     CDS             complement(1149..2291)
                     /locus_tag="KRH54_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBG8579737.1"
                     /translation="MDFKIEKYLLKKTEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(2309..4063)
                     /locus_tag="KRH54_002081"
     CDS             complement(2309..4063)
                     /locus_tag="KRH54_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBG8579738.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQNLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            4199..4405
                     /locus_tag="KRH54_002082"
     CDS             4199..4405
                     /locus_tag="KRH54_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579739.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(4410..5333)
                     /locus_tag="KRH54_002083"
     CDS             complement(4410..5333)
                     /locus_tag="KRH54_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579740.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRIVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(5588..7288)
                     /locus_tag="KRH54_002084"
     CDS             complement(5588..7288)
                     /locus_tag="KRH54_002084"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBG8579741.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(7751..8164)
                     /locus_tag="KRH54_002085"
     CDS             complement(7751..8164)
                     /locus_tag="KRH54_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG8579742.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(8230..10608)
                     /locus_tag="KRH54_002086"
     CDS             complement(8230..10608)
                     /locus_tag="KRH54_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG8579743.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKSGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            10927..12378
                     /locus_tag="KRH54_002087"
     CDS             10927..12378
                     /locus_tag="KRH54_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBG8579744.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(12582..15095)
                     /locus_tag="KRH54_002088"
     CDS             complement(12582..15095)
                     /locus_tag="KRH54_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG8579745.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNNYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADTALYDSKRQGRNRFKIV"
BASE COUNT         4857 a         2613 c         1777 g         5935 t
ORIGIN      
        1 tataaattga tataattata taatagataa gttaatattg aggagggata attatggaaa
       61 taagagtatt aagatatttt ttagcaattg caagagaaga aagtatatct ggtgcggcaa
      121 cctttttaca tataacacaa ccaactcttt ctagacaaat tatggaactt gaagaagagt
      181 tgggagttaa attattggtt cgaggaaata gaaaaatcac cttaacaaat gaaggtaggc
      241 ttttacataa aagagcagaa gaaattatat cattagttga taaggttgaa ttagaatttg
      301 acaatcccaa tgagattatt agcggtgata tatatatagg aagtggtgaa actgaagcta
      361 tgcgacttat agctagaact gcccatcatt ttcatcaaaa ataccccaat gtaagatatc
      421 acctttttag tgggaatgct gatgatgtga ctgaaaaatt agacaaggga ctgcttgatt
      481 ttggtatttt aattgaacct gcaaatatac agaagtatga ttatgcacaa attcctgcta
      541 ctgatgtatg ggggttacta atgcgtaaag atagtgccct tgcatcacaa agcacaataa
      601 atccaaaaga tttatgcaat atacctctta ttatttcaag acagacactt gttgataaag
      661 caatttctaa atggcttcaa gacagtaatg aaaaactaaa cattattgca acatacaatt
      721 tagtttataa cgcttctctt ttggtagaag aaggtctggg ctacgctctg tgtttagata
      781 gactcgtcaa tactacagaa aacagtaatc tttgttttag acctctatct cctcctttag
      841 aagctaaatt aaatattata tggaaaaaac atcaaatatt ttcaaaggca gcagaaaaat
      901 ttttacttca aatacaaaaa gatttttctt ctatcatgtt ggatacatct aagttggata
      961 cacctaaaga aaaataaatc ataataaaaa gtgagagtgt ctcaaaatga actttgcact
     1021 gtataaggca ctctttttta tacaaagtaa aatatatgtt catcatttca gcactaaaaa
     1081 agaggcttat ctctattttg agatagcccc acttaaccaa taaaattaaa tacaacttaa
     1141 caatatcctt acttatttat tccaagttct cttagacaag caatagtaat ttcatcttgt
     1201 ggatttaatt cataagctct ttctatatag taagttgcat catcaatatc accattattt
     1261 aaataagcca ttcctaaatt gcaaagtatt tctgggtctt cctttatctt agccgcttgt
     1321 tcaaaatact ctattgcttt atctaaatta ttcttataag cctcacaaag ccccaattcc
     1381 actatagtat ccacttgatt aggctttatt atcaatatct tctcaagata catcttagcc
     1441 tgctctattt cgcccatacc cttatatcca agtgctatca taaacagcaa gttccaccaa
     1501 tccatatgtt cttcttctaa tggaagtaac ttctctaatc cttccttaaa cttaccttgg
     1561 aatacaagtg tatatccttc ttcatattga actttaaagt ccatcttgcc cagattctct
     1621 tgtacctcag ctactaaatc agcatctaac cctagcctta acgcttcttc ccaagtcagc
     1681 ttagtcttta aatattgacc ttgattataa taatggtagc ctaagtgata ataacctaat
     1741 gcaaaattct catcaacatc aattaccttt tcaagcttag ccatggcttc aagtaagaat
     1801 gcattcattg ccttatcatc catatctttt tgatactgtt tagctatctc ttggcaaaca
     1861 atcgcatagt tatacattgc atctaaatca tctggataca ttgttataaa tgcttttata
     1921 tatattaaag aatctgtatg ttcaccaatc tcaaagtatt ttctcgacat atatcccata
     1981 tacgatttca aatccaaatc aatatctttt gataaagcat ttaaaaactt ctcatactct
     2041 gcattgtttt taaacttact atctattcca attatatata tcattgcatc tgctatagac
     2101 attgaattta acttatcttg agcagtcttt tcttttatcc cttttactaa tacctcattt
     2161 tttataggta catctaatcc accttttggc acttcatagc tttttaattt aaactctcca
     2221 ccatgtttta tagttataaa agctaattct tctgtttttt ttagcaaata tttttctatc
     2281 ttaaaatcca ttttatcctc ctaaatactt aaatctctac tttatgagtt ttagatttct
     2341 caaatatttt tttatcatca tactcaaata agactacatt ttctacttct cttatttctg
     2401 tatttatatc attttttaaa tccacaacat taaatctaaa tggctcaaaa tgaacatatt
     2461 ttattatatg tttagctggt ttatcaacaa aactaggtaa atatgttgat aagttatcat
     2521 tatcttgtaa aaatacatgg catctattct taaaatcatc cacatctagc ttattaaaaa
     2581 actgtggtat atttgatgtt ttacccagtg atatatagtc atattttaat atatcgttga
     2641 atacatcatt atctatgttg attttttcat tatagaaatc catcaatatt ttatataact
     2701 gattctttcc ttgtgccaaa tcaaagtagc cattttcatc aaaatacttg gcaaaaacct
     2761 caaagaattt aaaagggctt tttttataaa atctgcctat aatatatctc atagataaca
     2821 caaagttttt agaattgtag tatctctcca gtatatcttc tatatccttt agcttcaaag
     2881 tttcttcata gcttatataa ttattgtata aaaactcata tggagcataa tccttatatt
     2941 taaaaccatg ctcatctgca acttttctca tacctgtccc tttaatcatc tttaaaaagc
     3001 ctaattgcaa atgctctata cctagattaa atacatcatt aaaagaattt tcaaagctct
     3061 tataatcttc ataaggcagt cctgcaatta agtctaagtg ctgatgtata tttctataag
     3121 atgctacagt ttgtacaaca tgagacaact ttttaaaatc atcccttcta cctactgcat
     3181 caagtgtttt ttcattcgtt gactgaacac ctatttcaaa ttgaaaaaga ccttctttac
     3241 aatcagccaa aaactcaagc atcttatcat ctatcaaata cgctgtaact tcaaaatgat
     3301 aagttgtata tccattatca tttttcataa ggaaatccat gatttccata gccacttttc
     3361 tatttgcatt gaatgtccta tctataaact ttatttgtga tactcttgca tctattaaag
     3421 cttttaaatc actctttatc ctgtctaaac taaaatacct tagacctgga atagtagatg
     3481 ataaacagta ttggcagtta aaagggcaac ctcttgatga ttcataatac actattctat
     3541 tttcatattc ttttggattc aagttttcat aaggactagg aatctcatcc aaattttgaa
     3601 gtaattccat aggcttattc actattatct cattgccttg tctatataca agtccattta
     3661 catcatttat ttccatcttt ccttgcaagt gaagtactaa gtctctaaaa attaattctc
     3721 cctctccata aagtatataa tccacaaaat catacttttt cattgcctca taactatcat
     3781 aactcacttc cggtccgccc aaagctattt tagtattagg tctaattttt tttagattat
     3841 cacaaagttt aactatatct cccacattcc agatatatgt tgagaataat attatatcat
     3901 attcattttt atatatatcc tttaaaatat agtccaaatc gttgtttatc gtgtattctt
     3961 tcatattaac ttctatcaaa tctcttacaa attcttttag atatcttata gccaaatttg
     4021 tatgaataaa cttggaattt aatgttgtta ataaaatttt caaatatttc tcacctcttt
     4081 ttaagattat actgtctttt tgtacccctt ataggttaaa atagtatatg taaagtctaa
     4141 ataatatcta tataattaat ttattaattt caagtaatgt gaggaaagga agttttttat
     4201 ggaattaatt gaaaaagtat ctatcaactc tttatcaaaa agagatttac tacttattat
     4261 aaagtctctt gaatacacta atgaaaatac tcaaattagt gatttcattg accttaggaa
     4321 taatatagta aaggaattat gttttttaac tgacactaag gaaaaagatt ttttagatta
     4381 tctagaaaaa aatagcacac tctaataacc taaacctttt taaactttaa tatataaata
     4441 cctgatttag aatttatttt tatgcttaat atattatcat taaaggtttt cgacataact
     4501 gttatttgat taaatatagg gtctattata gtattacctt cttcaagtct tataagactt
     4561 cttgggtcta tgcttttatt atatgcctta actccaattt tatattttct caatttaaca
     4621 acatattcgg acatagaaaa aataaaatct atattatctt catttaattt actatcttca
     4681 cccaaaatgg ataatataga tttttttatg ctattaattc cttcattttc tacaattcta
     4741 tcttccatat atacatcata tagaacattt aaaacattta ataaaatact ttcttcatca
     4801 taatcttcag atttcaaatc aatgtactta tcaattaatt gtattgcttt aatccttgcc
     4861 atctgaaact ttacaacatc tgatttgtct aattctatag aaaatcctat tatcttgtct
     4921 ttaatactct gtagtaattc gacatactct attttactat tatttataag attgtgcata
     4981 atggaattat acaccacaga acctaataaa taatcaaata aaagttcttc ttttttaaaa
     5041 taaataacat acttttttat tagtttaata acctcatctt cacttttttg aatattagta
     5101 tttgataaaa ttagtggtaa taaaatattt aaaaaattga tatttcctat tttttctcca
     5161 taaaatctat agtaaataag ttcatctctc agtctatttt cgtctatata cacacatctt
     5221 tgtgttttat catctattaa ggattttgta tttattatag ttaaatcttt aagagcccta
     5281 cttgcactac taaaagaaga tgtgtagact ttttttagta attcgtcatt cataaatata
     5341 tcctttctga aaaattgaat gtaaataaat tgttattaaa aaaaaaagag acagcccgtt
     5401 taagaagctg tcatatatag ggggatagta gtgtacaaaa atcattgtaa tctactatct
     5461 atatattatt atacgatact acttttttat atgtaaagca tcagttttcg acatccctat
     5521 attttacagt tatacaaatt gcgtactaag ttcaatttta ttctttttat ttcaacattt
     5581 tatcatgcta cattctttca ggagcgtgca ttcccaacaa tttcagagaa ttttctaaag
     5641 tttgttttgt tgctaaaact aatgcaagtc ttgcttttct tatttccaaa ttttctacta
     5701 aaattggatt gtcatgataa aatttattaa acgcttgagc taaatctagc ataaatctag
     5761 tcactatatg tggctcattt ttctttagag caagtaaaat attcttattg aatgattcta
     5821 taactctaag aacttctgca ctatcttcat cagctaatag actataatct atatcagaag
     5881 tcacttctac ttctgctttt cttaaaactg cacagcatct agcatgtgta tactgaacat
     5941 atggtccagt ctcaccatca aaactaagcg ttctttccca agagaaggta tagtctttaa
     6001 ttctactatt agataactct tggaatacta ctgccccaac acctacttgc ttagatattt
     6061 catccacatt tttagcattt gggtttttag ctagtataat ttccttagtt ttatctatag
     6121 cttgatttaa agcatcttct aaaaatacta ctcttccttt tctagtcgac atagtacctt
     6181 cttcaagtgc aaccatacca aatccaacat gaattaaatc ttttgcccaa tcatatccca
     6241 taagctcaat taccttaaac cattgttgga aatgtaagtt ttgttgagaa ccaactacat
     6301 atatacactt gtcaaaatca taagtttctt ttctatatat tgcagcagct aaatctcttg
     6361 tcatatataa cgttgaacca tcattcttag taataagtgc tggtggcatt ttatactctt
     6421 ctaaatcaac aattcttgct cctttagact cttttaaaag atttttctct tctaatagtt
     6481 cgataaccct gttcatttta tcagaataaa aactctctcc tgctaaagaa tcaaattcta
     6541 tatctaaaag tttataaact ctgttaaact cttttaaact ctcatttcta aaccattgcc
     6601 ataattcttg tgcttcttcg tcaccatttt ctaatttatt aaaccatgct cttgcttcat
     6661 cttccatttc tgggtgttgt tcagcttcat catggaatct tacgtataat tttagcaatt
     6721 ctggaattgg attagactct actacttctt tttctcccca ctttttaaag gccactatta
     6781 acttaccaaa ctgagttcca tagtcaccta aatgatttat tcttatagtc ttgtatcctt
     6841 gcgaatcata tatcttatat aaagcattac ctataactgt agttcttatg tgccctatat
     6901 ggaaaggttt tgctatattt ggagaagaat attcaactat tacagtttta tcttttccaa
     6961 aatcactatg tccgtaattt tctttttcat ctaaaacttt tttaattaca gtttctgcta
     7021 attgagattt atttacaaag aaatttacat atccgcctaa ttgtattact tttgttatct
     7081 ctccacttgg ttctatactt tccgccaatt cacttgctat catgtttgga gcttttctaa
     7141 acactttggc taatttaaag catggaaaag catagtcccc catatcctta tttggtggaa
     7201 cttctataag tgcctctatc tcttctttcg ataaatcttc tattttttct ttaaggcaat
     7261 ttgatattgc caccttaaaa tcttgcattc tgttgctcct ctctacattg ttcctggttt
     7321 atattgtgct tatctcttta tgttaaatca aatttcgtgt tttttcaagt atttcattac
     7381 agtgtaaaac actttagtaa ttatgttttc tgtatatatt aaaaatacta taattgcagt
     7441 ttttaatcac actattattg attttttata agactttttt ctattttatt gtttcttgta
     7501 ctatccagtt gactttatat acttttttta atcttttatc tattttcata cgtcaatgct
     7561 aattttatat acttttcttt taatctttta ttaattttca tacatcaatg ttaactttat
     7621 atacgttttt ttaatctttt atctattttc atacatcaat gtcagcttta tatacttttt
     7681 ttactctttt atctattttc tataaatcaa tattagcttt atatactttt tttactcttt
     7741 tatctatttt ctataaatct ttttcactct taacttttat tccttcttct tctaatagcc
     7801 ttgctgttat acctttccct ggtatctttt tacctgaaaa actaccatca tatattagat
     7861 tacatccaca agaggggcta ccctctttta gtattgcttc gctacataca tataaatttg
     7921 ctatttttag agtttcatta gcccctctta caaagttttc tgttacatcc ttattatctt
     7981 tagtaattac ttttccatca actatctcag caggtggtct tggtgtagat agaccaccta
     8041 actgctctgg gcatacaagt gtaaattctt tgccttttaa atattccttg actttttcat
     8101 tatcattatt atcgccacta tatttacaat ttattcctat taaacatgca gataccaata
     8161 tcataaaaat gtgctcctat acaaattata tatattctca tacaaattac taatgtattc
     8221 ttatacaaat tattttaatt taacaattgt tactcctgct ccaccttctc catattgacc
     8281 acctctttgt gattttacat gtctatttct tcttaaaatg tcttgtagcc ctgcttttaa
     8341 tactcctgta cctattccat gtattactgt tacagactct aaacctgcca catatgcatc
     8401 atcaagatac ttttctactt ccattatagc ttcttctagg tttaaccctc taaggtcaac
     8461 ttcatttttt acactaccag attttgacct tataatgttt ctcgttgatt tagttacatt
     8521 tgttgaaaca tctttgcgtg ttttttgtaa agatttaaaa ggtagagtca tcttcattat
     8581 accaatttgc actactgctt cttttctctt tttatctact gaaactacac taccattttg
     8641 atttaaagtt attaccttaa cttcttcacc tggctttaag tcttttatct ccttatttga
     8701 tacctttgga actatcatac tttttactgt tggctgaaga cttcccatag agctagtcaa
     8761 ttcttttcta agttcttcta tcttacgatt cttttctttt gaagctcttt cttgttcaag
     8821 acttctaagt tcttttatta taatatctac ttcttcttta gcttgtctta taattgaaaa
     8881 tgcttctgat ttagcttttt caatcatctt atctctttga ctgaccaatt tttcaagttt
     8941 ttcgtcatac tctactttca gtttttcaat ctcttctttt aatctttcag cttcttctct
     9001 atcttctact gctttgattc tattcttttc aacattctgt aacacatctt ctaaagctat
     9061 gttttctgta tttatatatt ctttagcacg tgaaatcaca taatcactta gcccaagttt
     9121 tctagaaatc tcaaacgcat ttgattttcc gggaacacct ataagtaact tatatgttgg
     9181 gcttaaagtt tctatatcaa attcaactgc tgcattttct acacctgatt tagtaagagc
     9241 ataattttta agctcactat agtgagtggt agctatacat ttagctccta cagaatttat
     9301 atcttctaat actgctattg caagggcagc tccttcaaca gggtctgttc ccgctcctaa
     9361 ctcgtcaaat ataactaaag agtctgctgt tacgttttgt aatatagata ctatattcgt
     9421 catatgagat gaaaatgttg atagactctg ttcaatactc tgctcatctc ctatatctgc
     9481 aaatacatta tcatatacac acatactact tccataatca gcaggtatat gaagtccact
     9541 ttgtgtcata agtgcaaata gacctacagt ttttattgta acagtcttac cacctgtgtt
     9601 tggacctgtt atcaccaatg tatggaagtc tcttcctaaa taaattgtat ttgctactac
     9661 cttcttttta tccagcagtg gatgtcttcc attttttatg tttaaatatt tatcctcatt
     9721 taaagttggt tctattcctc tcatctgtat agaaagtttt ccctttgaaa atgcaaagtc
     9781 caatcttcca agtatctctt gattagaaat taagtcttca ctaacttctc caaccatagc
     9841 agaaagctct gataaaattc tttctatttc ctcttgctct cctagtttca actgcctcaa
     9901 ttcattgttc atctctacta tagtcattgg ctctataaaa agtgtcgcac cagaagatga
     9961 ctggtcatgc actataccag ctacttgcga cctatattca gattttacag gtactacaaa
    10021 tctatcacct cttagagaaa ttattgcatc ttgtaagtac ttttgataag tagttgaact
    10081 tattattgaa tttaattttg acctaatgga ttgatttttt tgggctattc ttcttctgat
    10141 atctctaagt gtacttgaag cattgtcaga tatttctact tcactaatta tagcattgta
    10201 tatctggtct tctatgtcct tgtatacata cagagaattt gatagagatt gtattattgg
    10261 ataattaaaa tcttcttcat cactactaga taagctattt cttaatcttc tagccactct
    10321 taaggtatct gctatcataa tcagacttcc tgggtcaaga gaagcaccta tataagctct
    10381 ctttacctta tcttctatat catgaatacc ttccaaccct actgaacctc tctttataat
    10441 aattgattga gcttcactag tttcttctag catagatttc acttctacaa aatcagtatt
    10501 tggtactaaa ttttctatat attttagacc taaagaagat gaggctttct tctttaacaa
    10561 atctattatt ttattgtatt ctagaactct taacgatttc tcattcattt tctatattca
    10621 ccatcctcga tattttaact tttttattta attttaaaaa tgtatgtttt caagcatttc
    10681 caataaaaac tgcaagtttt atttcttttt catttctaaa attattatat tttttctctt
    10741 ttagatatat tttacacata ttttgtatta tactatgtat gtagctatct atatattata
    10801 cataaacatg ctttatttgt aatataatct gttaaaaaat cttctttaat atttttaaag
    10861 gagtttattt tagcttataa aaaaaaatag ctacttatat agataaatta aggaggaaca
    10921 tgagtcatgg gaaacgtatt agaaggatta aaaccagaaa gtgtttttaa aaactttgag
    10981 aaaatatctc aaattcctag aggttctgga aatgaaaaag gcataagtga ttttttactt
    11041 agctttggta aaaatttagg tcttgaaact attcaggatg aaagcttaaa cataataata
    11101 agaaagcctg ctacaaaagg atatgaaaat tgtccaggtg tagttcttca aggacatatg
    11161 gatatggttt gcgaaaaaga aaagaatgtt gaacatgatt ttcttaaaga ccctataaaa
    11221 ttaagaattg atggtgacat gatatatgct actggaacta ctctaggagc tgacaatggt
    11281 atagctgttg ctatgggtat ggctatatta gaagataata ctttagagca tcctgcacta
    11341 gaagttttag ttactgttaa tgaagaagat ggtatgaatg gtgcagatgc actagaccct
    11401 agcttaatta aaggtcaata tatcctaaat atggactccg aggaggaagg atacttatta
    11461 gttagctgtg ctggtggtaa aacttgtgtt gttagtttac ctgttgaata caaggaagtt
    11521 aaaggtgaca aacaaggatt attagttgag gttactggac tattaggtgg acactctggt
    11581 atggaaattg ttttacaaag agcaaatgct aacaaagcaa tagctagagt attaagtgta
    11641 ttgaatgttg attatgaatt agcttctgtt gatggtggta ctaagcacaa tgctatacct
    11701 cgtgaagcta aatgtgtaat agctgttaat aaagctgatg ttgaatctgc taaaaaacaa
    11761 ataaatgaca ttttaactgc atttaaacat gagtttacta cttctgaccc tggcatgact
    11821 tactcagtag ctgaaacttc tgtagacaaa gttcttacta aagattgtaa agaaaaagtt
    11881 gttcaaatgt cttgcttaac tcctcatgga gttcaatcag taagtcttga tatagaagga
    11941 ttagttgaaa gttctactaa cttcgctata attgaaacta aagaatctac tattgaattt
    12001 ttaacttctg ttagaagttc tgttatgtct attagagatg aaatcgctga tagaataaga
    12061 cttttggctc aagctttagg tgctaattac gaccttatag ctcaataccc agcttgggaa
    12121 tttaaaaaag gctcaaaatt agaaaaaata tgcagtgaaa cttatgagaa attaactggt
    12181 aaagttccta ctgttatggc tctacatgct ggtcttgagt gtggattatt acttgataaa
    12241 cttcctcatg ctgaagctat ctctatagga ccagatatgt ttgatgttca tactccaaat
    12301 gaacatgtta gtatcccttc tgtagctaat gtttgggatt atgttattga aatattaaaa
    12361 tctatgaacc aatattaaat ttaagttttg attctaagta gattttaatt ttaagtaatc
    12421 tatataaaat taactctgta aattaatact actgttagta taatatttac tttcaatatt
    12481 aacttataga aataaagcct aatgatagat aataacatgt atcattaggc tttattgtaa
    12541 tttaaatatt attaagcttt attctgtttt aaaattaatt cttatactat tttaaatcta
    12601 tttcttcctt gtctcttaga atcataaaga gcagtatctg ctatttgata cagttcatca
    12661 tactcttgac caggctttgc tataactacc cccatactaa tagataaact ttctccattt
    12721 ggcaacttaa tgagatgaat atgattgtat acatcattac atttattaat aagttcttcc
    12781 ttatttatta tatcattgat ataaacacta aattcatctc cacccaaacg acctactata
    12841 tcattttttg caaaactttc atcaagtacc tttgcaacat caataagtac tttatctcct
    12901 aacaagtgac cataagtatc attaatcgac ttaaaataat caatatctat aatcaataaa
    12961 gcaccatttt tttcagttga taaattcaac aaattttgtg cagttaattt tctaatatat
    13021 gctgcattat aaatattggt taaactatcc ctttgagcct tatctaacaa tctattcttt
    13081 tcctcttttt cagaatcaat attacatatt ttcccgatta catacattgg tatttttcta
    13141 tcattataaa ttatttttga agtaactcta atccaatgaa aagtatcatc aagcataata
    13201 gaatacattt cagttgttcc attttcttgt gattttatta catcaaaaaa ctttttatta
    13261 tattctttcc ttattttatc tctttcattt tctatattgc cattttcact ttccttatca
    13321 tttttatcca aaccataata agtctcaata cccttttctg gcatagaaat tatcaattta
    13381 tcttgcttat aatcatactc aaaaaagtat tcacttgtca tttcatacaa ctgagtatgt
    13441 tttttgagtt ccaaagatat cttctcgttc atacttgtac gagtatgtaa accaaatgcc
    13501 ataaatccaa taatacaaat taataaactg gctacaataa aaatagtctc aattggattc
    13561 tgttgcataa tataaagcaa cgagaaatct tgtctatatg tagtattttg gtagacaatt
    13621 gattgcattt tttctgatga aatacttata atagctttat taaatatatt caataattct
    13681 tgtttatctt ttttaattat accaaaacaa atactctttg ctttataggt ttgaggaact
    13741 agacgcacat ttttatactt aggttgattc atatagtact gcatagaata accttcgcca
    13801 taagtgtaat ctgcatagcc tttattaaca gcttctatac actcttctaa attatcatac
    13861 caaactatat tctcttcaaa ctgaccataa taattaatac ctttggctag agctacacgt
    13921 tttccatcta aactattctc atttaattta ttattaatca tcatagtata ttgagtagaa
    13981 agaaaaggcc gagacatagc tatattatgt tcacgagagt attcataatc ataaatcatt
    14041 cctgctacaa tatcaatttc acgcttttta gtcatgtttt ctaaatcttc ttgagatttt
    14101 acaggcacaa gttcaaattc taagcctgtt ttatcacata tatgatttaa caaatcaatt
    14161 gatatacctc ttaattcacc tgtatcttta tctatatttt gaaatggtgg tttgtttaaa
    14221 agtactccta cttttaattt ttctgtctct ttaatgtaat tagtttcttc atcagataaa
    14281 aacatttgat tattctctgg tgtaaagtat ttttcatata aagtagttga aaaatatggg
    14341 tctttctgtt caatattatt aattgctgag ttaagctttt gagttaaatt tgtactacct
    14401 ttaggagtaa tgaaataaaa tggttttgat gaaaattttg ctacagtacg tacaccttct
    14461 attgggttca tactcacatt aagaagtaca tctgctttac cactctttag tgcttctagt
    14521 tgctcatcat ttgttttaca atataccaat tcaggtgaca taagattcat tttacaatac
    14581 tcatctaatt ctttaagtct agtatcagaa ttttctatta cagcaatacg gatttttttc
    14641 acatcttgtg aactcattat gttatcaata tctgtatttt catataatgc ttgtagtact
    14701 gtatttgcta ctccataatt attccctgca taattatata ttttttgcat tggttcattg
    14761 tataacatac ctcccattat atcaatttca ccattttcta acatcttcat taattttgta
    14821 atagattcat ttatctctcc aggaacttga acaaattcat aattccatcc agtatactgt
    14881 gcaatttcta gtaaatattc atatgtatac ccaaaatagt taccttgctc atctatttca
    14941 gttaaacctt tttgaatagg ataagcaaca cgaatggtac gattatcatt acctatagcg
    15001 ttgataccaa aagaaaatat gcaagaagtg aatatagatg taattacaat aattaataaa
    15061 agtaattttt ttaaattcac tacacaatcc tccatatagt taagatttac agtataaatt
    15121 actgcataaa cttgtagaca tgtaaaaata gattagtata taaaaggata tataaatcta
    15181 ct
//
LOCUS       DAEOSP010000088        15137 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.088, whole genome shotgun sequence.
ACCESSION   DAEOSP010000088 DAEOSP010000000
VERSION     DAEOSP010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15137
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.088"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            397..1230
                     /locus_tag="KRH54_002089"
     CDS             397..1230
                     /locus_tag="KRH54_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG8579746.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1323..2255
                     /locus_tag="KRH54_002090"
     CDS             1323..2255
                     /locus_tag="KRH54_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG8579747.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2307..2789
                     /locus_tag="KRH54_002091"
     CDS             2307..2789
                     /locus_tag="KRH54_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG8579748.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2801..3670
                     /locus_tag="KRH54_002092"
     CDS             2801..3670
                     /locus_tag="KRH54_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG8579749.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3713..4468
                     /locus_tag="KRH54_002093"
     CDS             3713..4468
                     /locus_tag="KRH54_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG8579750.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLVSLVDTNPIFAGSSFNETSNSD
                     TIKVLNTKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KEDNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4510..5331
                     /locus_tag="KRH54_002094"
     CDS             4510..5331
                     /locus_tag="KRH54_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HBG8579751.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDKNSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5336..6097
                     /locus_tag="KRH54_002095"
     CDS             5336..6097
                     /locus_tag="KRH54_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBG8579752.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6258..6515
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6628..6867
                     /locus_tag="KRH54_002096"
     CDS             6628..6867
                     /locus_tag="KRH54_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBG8579753.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            7171..8166
                     /gene="ilvC"
                     /locus_tag="KRH54_002097"
     CDS             7171..8166
                     /gene="ilvC"
                     /locus_tag="KRH54_002097"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBG8579754.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDVAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            8219..9907
                     /gene="ilvB"
                     /locus_tag="KRH54_002098"
     CDS             8219..9907
                     /gene="ilvB"
                     /locus_tag="KRH54_002098"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBG8579755.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPIIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            10118..10795
                     /locus_tag="KRH54_002099"
     CDS             10118..10795
                     /locus_tag="KRH54_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG8579756.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            10969..13005
                     /locus_tag="KRH54_002100"
     CDS             10969..13005
                     /locus_tag="KRH54_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBG8579757.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKISYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(13196..13762)
                     /locus_tag="KRH54_002101"
     CDS             complement(13196..13762)
                     /locus_tag="KRH54_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8579758.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKKD"
     gene            14050..14985
                     /locus_tag="KRH54_002102"
     CDS             14050..14985
                     /locus_tag="KRH54_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8579759.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
BASE COUNT         6261 a         1484 c         2652 g         4740 t
ORIGIN      
        1 atttataaat gagattagaa aattttattt gataacaaaa attagatagt atgaatatat
       61 aatgtatgta ttaaaaatgt ggcaaatagt tgttttgcta tttattattt gtttaatatt
      121 tctaatttat aaaaaaaaaa ttataaattt taaaaattat aaatgacatt ttaactagaa
      181 ttaattgtaa aaaacactat ataatactca attttcaaca ttctatacaa ttatacagaa
      241 attatacagc ataataatca ccgaaataac aaattttaaa aattaagcaa aactttttgt
      301 cgaagttcaa cgtctatata tatgtagtgt aaatttttgg gataaaatac tacaaaaata
      361 taaatgtaaa aatatagtaa tattggaggt cgaacgatga gaaataaaaa gaaaaaaata
      421 aatagaaaga agttgtattt gcttttaagt gctattgtag tgtgtctggc ttttatagta
      481 gttggtgttc gcgtcagctc tttaaatatg aaggaaaatg aaattagtag aaatgcaaaa
      541 ctgtcaagtt ctactattac agatatagta tctaatacat caatagaagt tagtaaaggg
      601 ccaagtaaaa gttctggaaa aatagcatac ataactatag atgatggtcc atctaaattt
      661 actgacgaga tgataaaaac tttaaataaa tacaatgtta aagcaacttt ttttatgata
      721 gatgggaata tgaaggaata tccacagcaa gttaaaaata taattaaaaa tgggaacaca
      781 gcaggttttc atagtgtatc acatgatatt cacaagcttt atgttactag tacatcagca
      841 aaagaggaat ttgatacaaa tgatcaaact ttttataaga taactggtaa acattcaaaa
      901 gtaataagaa taccttatgg aagcaaacca tatacaccac aagcctctta tcaggcttta
      961 gttgatgctg gatataaaat ttgggactgg gacttagata cagaagactg gagatctaat
     1021 tcttctcaaa ttgtacaaaa tgtaaaaaat catattaaga atagaaaagg tgaagataaa
     1081 gaccaacttg tagttttaat gcatgagaaa aaacagagtg cagaagcttt agattcagtt
     1141 ttaaaattct tatcagatga aggttatgaa tttgcgccag tagatcaaaa ccaaatacca
     1201 aaaaattatt ggttacgtaa tttagagtag ttaaaatata agaatattga ttgatatttt
     1261 tagaaattga gaataaaaat gagtgaggtg taggtatatt gaataaaaaa ctttatatag
     1321 gaatggtcgg aatattgtct ttgatgatgg ttggttgtaa taaaagttta gcaaaagtaa
     1381 atgatgtgga aatcacaaaa gaacagtaca aaaaaacaaa agcagtttta tctgctacta
     1441 ataattatat aaatgggcag tctttagatg aattagaaaa aactttggat aaaaaaggta
     1501 gaaataaatt agaaaatgtt ataatatcgt ttatggtgga taatgaactt ttatatcaag
     1561 aggctaagga taaaggacta actccaagta aaagtgaagt agactcaaaa tatcaagaac
     1621 tagaagataa gatgaatttg aatacaagct ataaggaaaa aatggacaaa gcaggtgttg
     1681 ataaagatta tttaaaacaa gaaatatcta gagatttagc aatagataaa aataaaaagg
     1741 cctttgaaga tagaataaac ataagtgata atgatatgga agcctactat acaagtcata
     1801 aaaaagattt taatgtagaa gaagtaagtg cttctcagat acttatatcg actttagata
     1861 aaaataaaaa agaagtaagc aaagataaaa aagaagcttt gaagaagaag gcagataata
     1921 tattgactaa aatcaaaaat ggagaaagtt ttgaaagttt ggctaaaaaa tattcggatg
     1981 ataaagcaac tggaaaaaat ggaggacagc taggatattt tacaaaggat gataaaaatg
     2041 ctgagtttac aaaggaagtg ttcaaattaa agaaaaatga agtttcaaat gtgtttgaaa
     2101 ctagttatgg atatcatata gtcaaagtta ctgataaaag agaaagacaa aaaagtttta
     2161 atgagtgtca gagtttaata agagaatcta ttttaaatga aaagtatatt gagcatataa
     2221 aaaaactgaa tgaagatgct aaaatagaca gatagaattt ctaacagctc aatcctagcc
     2281 tgtaatcgga gggagatttt acaggaatgt ttaagagttt aaagagaacg aaagttgaga
     2341 aatacattat cgataataaa gactcttttt atagaatagc atatagttat acgaagaatg
     2401 aagaagatgc cttagatgtg gttcaagaag ccatgtataa ggcactatac tcagtggaaa
     2461 atattaaaga agtaaattat ataaagacat ggttttataa aattttagtt agaacgagta
     2521 tagattttat tagaaaaaac cgtaaatata ataatatgac agatattgac cttatagatg
     2581 agactggaga gtatgataaa tatactgatt tggatttaag aagagctttg gaagaattac
     2641 ctattgaata caaatctatt ataatattaa gattttttga agatttaaag attgaagaag
     2701 tagcaataat attggatgaa aatgtgaata ctgttaaaac taggttatat actgctttaa
     2761 aaaaactaaa attaaaaatt gaagagtagg gataaaaaga atgacaagca aagaaaggtt
     2821 aaataagtta aaagatgagt atcataaaat tccaataccc aagaaattag atactataat
     2881 aaatcatgaa aaaatagaaa acatatatcg agagaaaaat aaaaaagtaa atagattaag
     2941 atttaaagtt gcaatagcct ttgcatgtat atttacagta ttagtaaata taagtcctgt
     3001 atttgcagac aatttttcta agatacctgt aattggagca atagtagaag taataactat
     3061 aaaaaattat agtttaaaaa gtgaaaatta tgaagctgag attgatattc ctaaaatacg
     3121 aggtttaaaa gataaaaact tagaacaaag attaaacagt agttttatgg aagatggaaa
     3181 aaggctttat catcaattcc aagagagaat ggagaagata caatcatcaa agaataaagg
     3241 atataaatct ttaagtttga gttatagtgt aaaaaataat tctaaaaaat ttctatcaat
     3301 agagatgaca aaaaatgaga tagaggcttc atcatatgtg agtaaggtac attatactat
     3361 tgataaaaaa agacaaatag ttttgacttt gccaatgctt tttaaagatg ataagtatat
     3421 aaaagttata agtgataata taaaagaaca aatgagagaa caaatgaaaa aggatagtac
     3481 aaaatcctat tttatagatc aaaaaaaaga tttgcctgtt gaagatttta aaacaattaa
     3541 taagtatcag gatttttatt ttaataaaaa tgaagacctt gtaatttgtt ttgatgaata
     3601 tgaagtagca cctggttata tgggtgcagt tgagtttgta ataccatata aagtaataaa
     3661 agatttgtaa attgaatagt agtttatata taaatatttt aagaaggaca atatgactac
     3721 atataaaaag cttaaaaaat ctatatttaa aattgctata ttttcaatat gcttattagt
     3781 ttcattagta gatacaaacc ctatatttgc aggtagttcc tttaatgaaa cttcaaatag
     3841 tgatacgata aaggtgttaa atactaagag tatcaacttt aaacaagaca attatgaagt
     3901 gaatgtgagt attcctaaaa taaaaggttt aaaagataaa actttagaga ataaattaaa
     3961 caatgagttt ttgcaagatg ggaaaaaaat ttacagtgaa tttcaaaata aaatgaaaaa
     4021 ggaagataat aagggtcata gatacctaaa tgtaatccac aatgtcaaaa ataattctaa
     4081 ggacttttta tcaatagaaa tgacaaaaga agagatagaa gcttcctcat ctataactaa
     4141 gaagcattac actgttgata aaaatagagg aatactatta aatttaccaa tattttttaa
     4201 agatagtaga tatattagag ctataagtga taatataaaa gagcagatga gaaatcaaat
     4261 taagaaagat gatacaaaat cttattttat agaccaagat aaagactcgc cagtgaaaga
     4321 ttttgaatct ataaataagt atcaagattt ttattttaat aaaaatggaa atcttgtgat
     4381 tagttttgat gaatatgaag tagcacctgg ttatatggga gcagtagaat ttgtgatacc
     4441 aaataaagta ataaaaagac taagatagat atatagaaga tttttagata gactggtaag
     4501 gagaattata tgaatatata tgaacaagca ataagattaa tagaaaataa tgaggatttc
     4561 gcatttgcta caataacgtc tcattcaggt tcaacaccac gtgaaactgg agcaatgatg
     4621 attgtgaaaa atgattctac tatatttggc tcagtaggag gaggtagtgt agaagcagaa
     4681 tgtataaaac atgcaattaa tgttataaaa aacagagaat caatgttgta taaatttact
     4741 ctaaacaaat ctgatgtggc aaaactagga atgatatgtg gaggtactgg agaaatacaa
     4801 atagatttta tagatagtaa attaaaaagt aatatagaaa aatttaataa gagactaaag
     4861 gaaaatacaa gtaaagcata tatatttggt gcaggtcata tttcaagaga tgttgctgtg
     4921 atactatctc ttttagaatt tagaacagta gtaatagatg atagagaaga atttgctaat
     4981 catgaaaggt ttccagaatc agaagtagta gttttagatt cttttgaaaa cataccagat
     5041 tttccaactg acaaaaatag ttatataatt attttaacaa gaggtcatct atatgattca
     5101 agtgctttag aatgggctct taaaagagag gctggttata taggtatgat tggaagtaga
     5161 acaaaaattg gattgactta cgaaaaactc atgaaaaaag gttttaagaa ggaagaatta
     5221 tcaaaagtac atgcaccaat aggcataaaa ttgaatgctc aaacaccagc tgaaatagct
     5281 gtatgtatag ctgctgaact tataaattgt agagctaata aggaaaaata aactaatgaa
     5341 cacagggagt aaagatattg atactttaaa ggttaatttt agagggattg aaataaaagc
     5401 agttttagta tataaaaata gaaaaaatat ttctataaaa atagatccat ttggcaaaat
     5461 aactgtaatg agtcctccta aaatatccaa aaaaataata aaagatataa ttattgaaaa
     5521 aggagactgg atacttaaaa agtcagaaaa atgtaaagga agagaggaag tatataaaca
     5581 aagaatgttt ataactggcg aaaaatttct ttatttaggt gaagagtacc atttagttat
     5641 aaaagaactg ttgagagatt ctgtcaaaaa aagtaactgt agaataacta ttaacgaata
     5701 tcaaatagtt gttcagacaa atgatacaag tacagatttt attaaaaaat ctttaaaatc
     5761 ttggtataag atggaaagtg aaagaatagt attagagaga atagattttc ttaaattaaa
     5821 ttgtgaaata atgaagcaat tagttccagc gagtgttaag gtaaaagaac aaaataagag
     5881 atggggaagt tgtacagctc aaaaaaatat ttatataaac tcaaaaatat ctatggcaag
     5941 attagatgta attgattatg taataataca tgagtttagc catttagtac atatgaatca
     6001 ctctaaaaaa ttctatgatt tagtaaaatc cattatgccc gattataaag ataaagaaaa
     6061 ttggctaaaa ataaatggat ataaaataat aatctgaaaa cgattattcc aaaatatagg
     6121 cgtaaagtat aaaagaagta ttattgtgaa aaaaataaat aatctgaaaa ttttaaaaaa
     6181 agtcattgac attgtaaatg atagggaata taatctatat taacaacaaa acagaattaa
     6241 aatataaaat taaaaaatta aaagcaatga cagagacaac aatactaata gtgttatcca
     6301 cagagaacca atgttgctga gaattggtgt taacagtagt ataatgatca ctctggagct
     6361 tttgtactga atatttttag taagtacaaa cgtatgtcag cgttacatga ctaaggttaa
     6421 ggtaattaat tacccgacct gaaaaaagag gggtagatgt attttttata tctacaaata
     6481 aggtggtacc gcggaatata actttcgtcc ttataagtaa tatttatatt gcttataaag
     6541 atgaaagttt ttttatttta agcgatataa ataaatttaa tccatataaa attaataaaa
     6601 aaataataaa aataaggagg gatttttatg gaaaatactg tatatgctaa attaaaccaa
     6661 ggaatagatt cttttttaag agtggctatg actctaagaa gaagagaggt tgatatacaa
     6721 tcaataagta tgactgtaga taatatgaat aattcaggta taaaattaat agtaaatgaa
     6781 gaaaagacat ccttagattc tgtcttaaac catatgaaaa aactacatga tataagagag
     6841 ataactgttg aaagaatgag acattaatta aatatatttt ataaagctaa agataaatat
     6901 gcttatttta aaataaaaaa ctaaagataa atattaaata agaaaaatct tagattaaaa
     6961 tataagtgtc attgaattta ttagtttatc acagttaata aatttattag tttgttgtag
     7021 ctaataaaaa ctgaaaattc taaaattata attgtaagat tatataaatt tagaatttaa
     7081 atctgttgaa taatattaaa acaatgataa tgagtatgaa tttctaataa aaataaatat
     7141 cataaaaata agaaaaagta ttgggaggaa atgaaaatgg caagaatgta ttatgaaaaa
     7201 gatgtggatt tagaagtttt aaaaaataaa aaggttgcag ttttaggtta tggaagtcaa
     7261 ggacatgcac atgcacaaaa tcttagagat aacggtgtac atgtagtgat aggtctttat
     7321 gacggaagta aatcagcaca aaaagcaaaa gaagatggat ttgaagttaa gagtgtagca
     7381 gaagctacaa aagaaagtga tttgacaatg atgttaatgc ctgatgaaaa acaaaagaaa
     7441 gtttatgaag agagtgtaaa ggataactta aaagaaggcc agacattagc ttttgctcat
     7501 ggattcaata tacattataa tcaagtacaa ccaccagaat ttgttgatgt tgtaatggta
     7561 gctcctaaag gacctggtca cttagttaga aatgtattta caaagggaag tggagttcct
     7621 gcactatttg cagtatatca agactacact aaaaaagcta cagagacagt tttagcatat
     7681 gcaaaaggga taggagcaac aagagcaggt gttttagaaa ctacttttaa agaagaaaca
     7741 gaaacagatt tatttggaga acaatcagta ctttgtggtg gaataagtga acttataaaa
     7801 ttgggatata aaacgcttgt agatgcaggt tatcaaaaag aagtagcata ttttgaatgt
     7861 ttacatgaaa tgaaactaat agtcgacctt atatatgaag gtggatttga aagaatgaga
     7921 tatagtataa gtgacacagc agaatatgga gattatgtat ctggtaaaag ggtaataact
     7981 gatgttgcaa aacaaggtat gcaaaatgtt ttagaggata tacaaaatgg aaaatttgca
     8041 aaagcgtgga ttaaagaaaa tgaagaagga cgtgaaaact tcctaaaaac tagagaagaa
     8101 gaatataata cagaaattgc cgaagttggt agaaatctaa gaagtatgat gtcattctta
     8161 aaataaatat atattaaatg gattagtttg agggaaagag tggtgtatag tagtgcgtat
     8221 gaatggagca aaggtaattt tagaatgttt aaagaaagag ggaattgaca caatatttgg
     8281 atatcctggt ggagccgtga tacctcttta tgatgctcta tacgattatt cagatgattt
     8341 taaacatata aggacatccc atgaacaagg tttagttcat gctgcagatg ggtacgcaag
     8401 aagtactaat acagtaggag tttgttttac aacatctggt ccaggagcaa caaatgccat
     8461 aactggaata gcaacagctt ttatggattc ttcacctatg gttgtaattt cagggcaggt
     8521 gccaactagt ttattaggaa aagattcttt tcaagaaatt gacataactg gagcaacatt
     8581 atctatgaca aaacataatt atttagttag aaacactaaa gaattagttc ctacaataaa
     8641 agaagctttt agagttgcaa attcaggtag aaaaggacct gtacttgtag atgttccaaa
     8701 agatttattt ttagcagaaa tggattttag tggtgaggac tatgatttat gtcaaataga
     8761 tgattatatg gattataaga gtgattttga tctagatgat gaaactaata ttaagctttt
     8821 aaatgaagca atagatatca taaaagaatc taaaaagcct ataatatatg caggtggagg
     8881 tgttaaatct tcagacagtg aggaaattct tgaaaaattt gcaactaaga tagatacacc
     8941 cgtactaaat acacttatgg ggcttggaaa tatagataga aagaatgaat tatctcttgg
     9001 aatggttgga atgcatggaa gtagagaaag taatttagca ctttctaatt cagatttagt
     9061 aatagcgata ggtgcgagat ttagtgatag agtgataagt aaaagttctg aatttgctaa
     9121 aaatgcaaaa ataatacaca tagacataga cccatctgaa ataagtaaaa atatagaatc
     9181 taatgtatct ttggttggag atgtaaagct agtccttagt ttattaattg aaagggttga
     9241 aagtaagaat aatagcaatt ggaaagaaga gataaagaga tttagaaaga gtgagggtgt
     9301 acaaacatat gaatttcatc cacaaaatat acttaaaaaa ataaatgaaa aatatgaaac
     9361 attaaagaaa cccactgtgg tagtaactga tgtcggtcaa catcaaatgt gggctgctaa
     9421 gtactggaat tttaaaggta ataaaagttt tataacatca gcagggttag gaactatggg
     9481 atttggttta ggtgcagcta ttggaactaa agttggtaat gtagataaaa atgtggtttt
     9541 agtcactggt gatggaagtt ttagaatgaa ctgtaatgaa ctagctacag ttgcaaatta
     9601 taatgtacct atgcttattt tactactcaa taatagaaca ttaggaatgg taaggcaatg
     9661 gcaaaagtta ttttccaatc aaagatactc tcaaactgac attaatgaaa atgtggatta
     9721 tgttaaactc gtaaatgcat ataacattga tggatataag gtatccagta tggaagaatt
     9781 aggaaaggcg ctagatatga tagattttaa taaaccagta tttttgcaat gtgatataga
     9841 taaggattat gatgtttatc ctattgtagc acctaatgat gcattggaaa atttgatatg
     9901 taactaaaac ttatactgaa tattgataaa attttttatt aaatttttat tagtattcag
     9961 tttttgttgt ttagagatag attggcattt tttatttttg caaataaatc aatgactata
    10021 taattaagaa cttattaaat tttaattata caatttagaa tagtattaca acttaagtaa
    10081 ataataattt tgaataataa tttacggagg tattcatatg tttaaacacg ataaagcatt
    10141 attaaaagaa gtaaaagtag aaagaccaaa tcctcaatat gctgttttaa tgcaagaaca
    10201 gttgggtggt gcaaatggag agttaaaagc agctatgcaa tacttgtccc aaagttttag
    10261 aataaaagac cctcaaataa aagatttatt tcttgatata gcagcagaag agcttagcca
    10321 tatggaaatg gtagcacaaa ctataaattt attaaatgga catgatgttg attataatgc
    10381 agttaataca ggcgaaatag aaacacatgt attaactggt ctatcaccag tcttaataaa
    10441 ctcatcagga gctccttgga ctgctaatta cgttacagtt acaggggatt tagtagctga
    10501 cttactttca aatatagctt ctgaacaaag agctaaggta gtttatgagt acttgtatag
    10561 acaaatagat gataaatatg taaaagaaac aatagatttc ttacttaata gagaagaagc
    10621 acataatgct ctatttagag atgctttaaa taaagttaaa gatacaggtt caaatagaga
    10681 ttttggagtt acagaagatt ctaaattata tttcgatttg tctactccag ggccaaatca
    10741 cgatactaag atagatatta atcctccttc ttttgaaaaa cctttaaaaa aataatttga
    10801 tattttttat gttgttttgt tttagtagtt ataaaaatta tttactagaa gaattaatta
    10861 tatgaaaaat taaatatgta tagttaaaaa tgggtataca cttattataa gtgtatatct
    10921 atttgtttat tgcaaaacat ataataaaat aacaaatgag gtggtatttt ggacagtaat
    10981 agaaaaccta ataacctaat aaatgaaaaa tctccatatc ttttacagca tgcttataat
    11041 cctataaact ggtatagttg gaatgatgag gcttttaaaa aagcaaaaga agaggataaa
    11101 ccaatatttt taagtgtagg atattcaaca tgtcattggt gccatgttat ggaaaaagaa
    11161 tcttttgaag atgaagaagt tgctgaaata atgaatagaa actttgtggc aataaaagta
    11221 gataaagagg aaagacctga tgtagatagc gtgtatatga cagtttgcca agctatgaca
    11281 ggtagtggtg gatggccaat gaccataata atgacaccag ataaaaaacc tttttttgcg
    11341 ggaacatatt tcccaaaata ttcaaggtat aataggcctg gagttataga tttattagag
    11401 aatgtatctg aaaagtggaa tacaagcagg gatatattaa taaaatctgg agatgaaata
    11461 atagaagctc ttaaggatga ttttggtgta aaaaatactg agggtgattt atcaaaagag
    11521 atgttgagtt catcagttag agtatttaag gctatttatg atgaaaagta tggtggtttt
    11581 ggtaatgctc ctaaatttcc aagtcctcag aatcttatgt ttttaatgaa atattatagt
    11641 atagaaaaag acaaagatgt gttaaaaatg gtggaaaaaa ctttagatgg aatgtataga
    11701 ggaggcttgt ttgatcatat tggatttggg ttttcaagat actctactga taaaaagtgg
    11761 ttggctcctc attttgaaaa aatgctatat gataatgcta tgcttactat agctttttta
    11821 gatgcgtata aaataacaaa gaaagagttg tataaagaaa ttgctataaa aactatagat
    11881 tatgtagtta gagagatgaa ggataaagaa ggtgggtttt attcagcaca ggatgctgat
    11941 agtgaaggtg aagaaggtaa attttataca tttaatcctc ttgagataat tgaagttttg
    12001 ggtgaagaag atggaacttt ctttaataat tattttgata taactagtag tggaaatttt
    12061 gaaggcaaaa gtattcctaa tcttataaaa aataaagaat atgagagaca taatgaaaag
    12121 atagctgacc ttagtaaaaa ggtatttgaa tatagaaaag aaagaacatc tttgcataaa
    12181 gatgacaaga tacttacatc atggaatgca ttgatgatag ttgctttaac aaaggcatat
    12241 agtactttaa aaaatgacat atatttagag tattcaaata agtgtcttaa ctttataaat
    12301 aataatcttg taaatgagtc aggtagatta ttagctagat atagagatgg aagttccgac
    12361 tatttagcat atttagatga ttatgctttt ttaatctggg cgtatataga gctttatgag
    12421 tcaacattta atatgaaata tttagaaaag gctttaaatt tgaatgaaag ctgtatcaac
    12481 cttttttggg attatgaaaa aagtggattt tatatatatg gtaaagatag tgaaaattta
    12541 atagcaagac caaaagattt gtatgatgga gctattccat caggaaattc tgttcaatta
    12601 tacaatctta taagacttgc gaaaattact ggagataata gacttgaaga aatgtcatat
    12661 aaacaattaa aactgtatgt ggataatgta aagagttctc caactggcta cagcttttat
    12721 atgctttcat tgatgtttga actttattct actaaggaaa ttatatgtat ttttaaggaa
    12781 gattctgatt taattgcatt taaagaattg ataagtgaaa attttatacc aaatgcaact
    12841 tttttagcaa agaaatacaa tgaagaaaac actattatag gttttttaaa taactataga
    12901 ttgaaggatg ataagattag ttattacgtg tgtcaaagta atagttgtag tcaaccaatt
    12961 aatgatttac aaaaattaaa agatatgatt ttaggagtag aataatcata atagcgaaaa
    13021 aggagatgct ttaaaataaa agtctaatca tctccttttt ttgtacaaaa aacagaaaaa
    13081 gagttcctaa gaacacctaa tctatgtaat gattgttttg ttaaaaaata aaaatgacat
    13141 tatagttata taaaatattg tactataaaa aagtggtatt atagatgcga tagtattaat
    13201 cttttttatt tactctattt cttctttttg caaattcttc aagtgcttca gatttaattt
    13261 tatctatttc ttcatattcc ataaaattag catcttcttc aaagccatct tgttcttcac
    13321 cattaaaacg atacattttt tttagtaaac tccataaggt ctctaattca tctttggtaa
    13381 attcgtgaaa tacatctgcc ataaagttta ttccagtcct actacatgtc accataacct
    13441 tttttcctaa gtctgtaact ttaacattaa ttgctctctt atcatgagga cttggaatta
    13501 catcaacata tccatttttt tcaagattag caactagtct atttatattt tgtttacttg
    13561 tacccatttt tcttgcaata ttatttaatg ttgtttcttc ctctggtaaa tgaagaattg
    13621 ataaaatagt catatactgt cttgaagtaa gtataccaaa atatttatct ccttctattt
    13681 gtattttgtt tgtaagggta aaaagagttg catatgtttg attcattaaa aagagctctt
    13741 ttagttcatt tgaataatcc atgaaatata tctcctttat ttttagatgt tgttttgtcg
    13801 aaaacagaat atcatgaatt aattctaatg tcaatatggt gtcaaaatat attaaagtat
    13861 tactttttta tgtaactatt aattaattaa attattgtat aattttatgt aaaaacttct
    13921 atgtaccaat gataacttct atgaggaatt aataattctt aaatttttta gaaagatttt
    13981 gacttgaaaa tacttgtaat ataatatata aattaatata ctgaataaaa tcaatctgga
    14041 gggataatta tgaagtgtac aaactttact ttcaaaggag aggaaggttt agatatatat
    14101 acatataaat gggaagatga aaatataaaa aaaccaaagg cagttatcca aattgcacat
    14161 ggaatggctg aaactgcaca aaggtatgag acttttgcca aggtacttac taaaaatggt
    14221 tatatagtat atatcaatga ccatagaggt catgggaaaa ctgcaaagat aatagaaaat
    14281 gttggacatt tagctgaaaa agaaggattt agatgtcttg tagaggacat gtatacttta
    14341 acaaatatta taaagaaaga aaatgaagat ctgccaattt atctatttgg acacagcatg
    14401 ggttcatttg caagtcaaag atatataatg gattacagta ataatttatc tggtcttata
    14461 ctatgtggtt caaatggaaa gcaaggaatc attctaaatc ttgctcatct gataatcaat
    14521 cgtgagatta aaaagtatgg aagacgttct aaaagtaata aaataaataa tttgatattt
    14581 ggcggcgaaa taataagaag aaatgaaaag actaagtttg actggttaag tagagataaa
    14641 gaacaagttg aaaaatatat aaatgaccca ttttgtggag tagtgtgtag ttgtggcttt
    14701 ttttatgatt tagttcaagg gttaaaagaa attgaggata aagaaaactt aaaaaaagtg
    14761 ccacttgata tacctattta cataatatct ggagataaag accccatagg aaaaaatggt
    14821 aaaggagtct taagactaag ggatagatat ataaaattag gagtaaaaga tgtaacttgc
    14881 aaactttaca aagatggaag gcatgaattg ttgaatgaaa taaatagaga agaagttttt
    14941 gaggatataa tttgttggtt aaataataaa atagaaatat tgtaataaaa aaatataatg
    15001 atatatttgc tttaaattta tttatatata atatttgaaa agtggattat gtatatgcaa
    15061 agtagaaaag agagcccaga agtgattttt tgctataatg ccatttattg aataggtggg
    15121 tgttattata aataaat
//
LOCUS       DAEOSP010000089        15053 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.089, whole genome shotgun sequence.
ACCESSION   DAEOSP010000089 DAEOSP010000000
VERSION     DAEOSP010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15053
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.089"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(246..389)
                     /gene="scfA"
                     /locus_tag="KRH54_002103"
     CDS             complement(246..389)
                     /gene="scfA"
                     /locus_tag="KRH54_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBG8579760.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(455..817)
                     /locus_tag="KRH54_002104"
     CDS             complement(455..817)
                     /locus_tag="KRH54_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBG8579761.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEIGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(875..1168)
                     /gene="yajC"
                     /locus_tag="KRH54_002105"
     CDS             complement(875..1168)
                     /gene="yajC"
                     /locus_tag="KRH54_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBG8579762.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(1302..2423)
                     /gene="tgt"
                     /locus_tag="KRH54_002106"
     CDS             complement(1302..2423)
                     /gene="tgt"
                     /locus_tag="KRH54_002106"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBG8579763.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(2502..2987)
                     /locus_tag="KRH54_002107"
     CDS             complement(2502..2987)
                     /locus_tag="KRH54_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579764.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(2999..4024)
                     /gene="queA"
                     /locus_tag="KRH54_002108"
     CDS             complement(2999..4024)
                     /gene="queA"
                     /locus_tag="KRH54_002108"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBG8579765.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEYGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(4342..5361)
                     /gene="ruvB"
                     /locus_tag="KRH54_002109"
     CDS             complement(4342..5361)
                     /gene="ruvB"
                     /locus_tag="KRH54_002109"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBG8579766.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(5468..6070)
                     /gene="ruvA"
                     /locus_tag="KRH54_002110"
     CDS             complement(5468..6070)
                     /gene="ruvA"
                     /locus_tag="KRH54_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBG8579767.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(6063..6572)
                     /gene="ruvC"
                     /locus_tag="KRH54_002111"
     CDS             complement(6063..6572)
                     /gene="ruvC"
                     /locus_tag="KRH54_002111"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBG8579768.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(6900..7529)
                     /locus_tag="KRH54_002112"
     CDS             complement(6900..7529)
                     /locus_tag="KRH54_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579769.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            7732..8055
                     /locus_tag="KRH54_002113"
     CDS             7732..8055
                     /locus_tag="KRH54_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBG8579770.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            8323..9177
                     /locus_tag="KRH54_002114"
     CDS             8323..9177
                     /locus_tag="KRH54_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579771.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(9363..10799)
                     /locus_tag="KRH54_002115"
     CDS             complement(9363..10799)
                     /locus_tag="KRH54_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBG8579772.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTKGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISRGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(10870..12252)
                     /locus_tag="KRH54_002116"
     CDS             complement(10870..12252)
                     /locus_tag="KRH54_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG8579773.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(12388..13293)
                     /gene="garR"
                     /locus_tag="KRH54_002117"
     CDS             complement(12388..13293)
                     /gene="garR"
                     /locus_tag="KRH54_002117"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBG8579774.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGIDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHDN"
     gene            13689..14360
                     /locus_tag="KRH54_002118"
     CDS             13689..14360
                     /locus_tag="KRH54_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8579775.1"
                     /translation="MATFKRLQEQAYDYLKELILSGKMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            14463..15011
                     /locus_tag="KRH54_002119"
     CDS             14463..15011
                     /locus_tag="KRH54_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579776.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
BASE COUNT         4951 a         2633 c         1740 g         5729 t
ORIGIN      
        1 aaaattaaaa attattctaa tacatgtaat tgataaaaaa taaaatataa gagtagctac
       61 tttaaaataa atgctcttac atgattagat atttttgcat aattaaacga taagaaatca
      121 aatttacttc tacataaata gaatatatat ttttatataa taattgaaaa taaaagagga
      181 tacctcataa tgataaaaga atcactttga gataccctct tacaaacaac taaatcaata
      241 attttctatc tttcacactc ttgatttgca actgtacaag aagttttaca agctgattga
      301 caagaagttt gacactctcc acatccacct tttgcagcac tttgctttaa agtagcttga
      361 gataaagttt ttacatgttt attttccatt ttcaacgcct ccatttctaa ttgtttccta
      421 ttgattatat cataaaacta ctttaatata aactctattt aaagttgaca cccaatacgc
      481 ctccaatgcc tcctgctgct gatattagca ataacttata aatcatccct atttcaaata
      541 caaacttttc ttgtgctaaa aacacagtca aaaaaataca tactatgtac aaaagtccta
      601 ctaaaagccc atacataagt cctttttctt tcatgtgttt tgaagtgtaa aaccctccta
      661 tagctgcaga aatagtagtt ataaatgatg agaccatagt tatattatct ccaccaatat
      721 ctgtaaatgt caaaaataag ttatacacta ataaaacagc caaagttatt atatatgcat
      781 atcctagacc tttaaaaata taattagatt ttcccataaa aaaacctcct ctcaaattaa
      841 tatatattcg agaaaaggct tttaaattac taatttagtc ttcttctatt tcttcttttt
      901 tagatttaac agttcctata gcccattttt caaatggaac tttagttcta tttggtccta
      961 attctaatat tacaacatca tcttcaactt tagctacatt agctactatt ccacctattg
     1021 taatgacctt gtctcctaca cttaggctgc ttctcatttc ttttaattgt ttgtcttttt
     1081 tcttttgagg tctaatcatt aggaaataaa agattgcgat tactactacc catagaccta
     1141 tactcataat tatttgttgc tgtggcatta atttttcact cctttgtaaa ttatactctt
     1201 ttgtaaatca taataaatgt ttatataaca atttactttc tctatctttg agtactattg
     1261 aatattatat attaaaatga ttataattaa tagtgcaaaa cctatatttc atatccatat
     1321 tttgcaaaaa attcatttct aaaatcaagt aatctatctt ccattatcgc ttgtcttatt
     1381 tgtttcatta aatttaataa aaagtgaaga ttatgagtag ttattaatct tgcacccagt
     1441 atttcatttg cttttataag gtgtcttata taagctcttg aatagttttt acaagcataa
     1501 caatcacatt cagggtcaag tggagtaaag tcttcagcat aagttgcatt tctaactact
     1561 actttacctt ggctagtcat tgcagttcca tttctagcga tacgagtagg tagaacacaa
     1621 tcaaacatgt ctactcctct tataacacct tctactaagt catctggact accaactccc
     1681 attaggtatc ttggtttatc ctttggcata agtggagttg tatgctctaa cacgtcatac
     1741 ataagtggtt ttggttctcc aacactaagt ccaccaatag catatcctgg taaatcaata
     1801 ttagttattt ctttcgctga ttgttccctt aagtctttat acatcccacc ttgtattata
     1861 ccaaataaag cttgcttgtc ggtattatta tgggcatctt tacatctttt taaccatctt
     1921 gttgttcttt ctaaagaatt ttttacatat tctctatctg atgggtatgg tgcacactca
     1981 tcaaatgcca tcattatgtc tgaacccaat gcattttgta tttccatagc tttttctgga
     2041 gttagaaaat gttttgaacc atctaagtgt gacctaaatt ctacaccttc ttcttttatc
     2101 tttcttaaag gccctagact aaatacctgg aaccctccac tatctgtaag tattggttta
     2161 tcccaattca taaacttatg aagaccacca gctcttttta taagttcatg acctggtctc
     2221 atatataaat ggtatgtgtt actaagtatt atttgtgaac caatctcttt taattcttct
     2281 ggtgtcatag attttacagt tgcttgagtt ccaactggca tgaatattgg tgtttcaata
     2341 atcccatgtg gagtgtgtaa tcttcctaac cttgcaccac tctgcttaca agtctttatc
     2401 aattcgtatc ttactgcgta cataaagttc tccttaatca tatttatttc tcattatttc
     2461 ttaagatata tttgtaataa gctattcata taatttattg tctaaataga tttatcttcg
     2521 tcttctttgt tctgttgttc cttaattgct tcttcatcat gaatactcat tatattaaga
     2581 gcactcttgt cttttgttac taaaaacttt tttatagcaa atagtcctgc tatagctata
     2641 actcctatta aaacatctat cataccttca tttttaggca gtagcaacat ttttctagca
     2701 attgcgtaaa gtaatgcctc aataaatgct cctggtatat gaagagatag cattactaca
     2761 agttcaacac ctattactaa aagtaatatt tgtgctaaaa aatcattaag ctgactgtat
     2821 tgtactggat tttgaaaatc aactatatat gctccccaca taagtcttag aacatctata
     2881 gttcctaaaa atactgctat tagaactact actgctaaaa cacgttcaaa tataaaagca
     2941 atctttaata tagggttatt ctttttttgt ttttgtaaac tatgcatcca ttacctccct
     3001 actttataat catagcatct ccaaaactga agaatctata tctttctttt actgcctcat
     3061 tatatgcatt taaaacatta tcctttccac aaatagcact tactaacatt attagtgtag
     3121 actctggtaa gtgaaaatta gtaataagtt tatctaaaac tttaaattta taacctggat
     3181 atataaatat ttctgtccat ccactagttt ctttcatctt tccatactca ttacaagctg
     3241 attctatagt tctacaactg gtagtaccaa cacatattac attttttcca ttttcctttg
     3301 ctctatttat tttgtcagct gcttcttggc taaccatata atattctgaa tgcatcttgt
     3361 gatttaaaac atcctctact tttactggtc taaaagttcc taagccaaca tgtagagtta
     3421 caaaggctat atctacacct ttttctttta ttttatttaa caattcttct gtaaaatgta
     3481 atcctgctgt aggtgctgct gctgaaccat tatgctttga ataaacagtc tggtatcttt
     3541 ctttttcatc tagtctagca gtgatatatg gaggtagtgg catatttcca agttcatcca
     3601 atatttcttc aaaaattcca tcataatgga attttataat tcttgaacct tcatctgata
     3661 aatctacaac ttctccaatt agctttccat ttccaaaaga aaatttagtc cctacttttg
     3721 ctctttttcc tggttttact aaagcctgcc aagtatcttc ctctgttctt tttaataata
     3781 aaaactctat tttacctcca gtttcaagct tctctcctat taatctagct ggtataactc
     3841 tagtattatt taatactaag caatctcctg gatttaaata ttctattata tctctaaata
     3901 ctttatgttc aatattacct gtctctttat ctaataccat caatcttgag ctagctctat
     3961 cttctatagg aacttgagct ataagttcct gtggtaagtc aaatttaaaa tcacttgttt
     4021 tcaatgttaa acttcctctc ttaaaatttc tccagctaca ataatatcaa aaaaacaact
     4081 tattttccac aatattacca taatctatca agaaattgct atttttaaca gacaccatat
     4141 ccacaatata tagatatttt tccacgatat acatgtctta tccacaatat atagatacaa
     4201 tacacacaat ttttatcgtg tttatccaca gtatctacag atataattac cattttgtgg
     4261 atatgtttga atttttagcc attttagaaa tattttaatt taatgactct tttattttat
     4321 aaatactcgt ataatctatt tttatttttc attaggatat ggaattttta agtgttcata
     4381 tgctaatggc atagctactc ttcctctagg acctctattt ataaatccta attgaagcaa
     4441 atatggctca tatacatctt ctattgtatt tctatcttct ccgatagaag ctgccaatgt
     4501 atctaaaccc actggtcctc ctctaaattt ttctattata gtcataagaa gcttctcatc
     4561 tacaaaatcg agacccaaac tatcgactcc taaaagttcc agagcatctt ttgccacctt
     4621 atcagttatt tttccatcag ctctgacttg tgcaaaatct ctaactcttt ttagtaatct
     4681 atttgctatc cttggagtcc ctcttgaacg ttttgcaagt tctaatgctc cattagactg
     4741 tatctctgca tctaagatgc ttgaagacct aagtactatt tttgaaagtt catcaactgt
     4801 ataatagtcc aatttacaaa taacaccaaa tctatctcta agtggatttg taagcatacc
     4861 agctcttgta gtagctccaa taagtgtaaa tttaggaagg tcaagtctta tgcttctagc
     4921 agatggccct ttccctatta ttatatctag gcaaaaatcc tccattgctg gatataaaac
     4981 ttcttcaaca cttctattga ttctatgaat ctcatctata aataaaacat cattttcatt
     5041 taaattcgta agtatggctg ccaaatctcc agctctttct attgcgggtc ctgacgtaat
     5101 tcttaaattt acccccatct catttgctat tatacttgct agtgtagttt ttccaagacc
     5161 tggtggtcca tataaaagca catggtctaa ctgctcattt ctcgatttag ctgcttctat
     5221 aaatatacta agttgttctt tggatttttc ttgacccaaa taatcttcta aagtttttgg
     5281 tcttaaacta ttttctatat ctatatcttc catcttcatt gtagaagtta ttatcctatt
     5341 ttcatcttca aaaccttgca tttatacccc tcctctcata cttttgaaaa tctactttat
     5401 atattttcaa atttacataa actccttaaa tattgtttta aaatccaaat attataaaat
     5461 ccaaatacta tttcaaggat ttactcatta tatatgtaag tgctttcttt ataatagctt
     5521 ctgtattcat accatctttt ttacattttt gaactgcctc ttttgattcc gacaaagtat
     5581 agcctaaagc tacaagtgca tctacagact catcttgtga tatcaaggta ggtttttgtg
     5641 ataataacgt tggttcaaac tctatgttat ttttgtctac cttatcctta agctctaaga
     5701 cgattctttc tgctgtcttt ttaccaacac caggagcttt ggataatttt ccaatgtctt
     5761 cacttaaaat atatgctcct aactgtgctg gtgatgcaaa tgatagtatt gaaagcgcca
     5821 ctttaggtcc tattttagat acagatgtta gtaattcaaa catcttaagt tcttccctac
     5881 ttacaaaacc acaaagactc atatcatctt ctcttacaat taatttagta tatatttttg
     5941 tggcttctcc cacttgtaaa ttcattattg tattggatga tacatttatt ttatatccta
     6001 tcccattatt ttcaacaact atactgtcta tataaatttc ttctactgta ccttttatat
     6061 aactatacat acttaccacc tatattcttt agagtttttt caagtttgtt tgaatgagca
     6121 tggcaaatag ccacagctaa agcatctgca acatcatctg gttttggaac tttttttaga
     6181 cttaaaaatg aagttaccat ctgttgaact tgtgccttat ctgctctccc atatccaact
     6241 acaccttgtt ttacttgaag tggagtgtat tcatatacag gcttcccatt atgagcacat
     6301 gcaagcatag taacacctct agcttgtgca actgttatag ctgtttttac attcttgtta
     6361 aagaataatt cttccattcc aacttcatct atattgtaat tctttactat tgtatcaatt
     6421 cctttataca caagttccaa tcttctcgat atattcatat gggcaggtgt tgtaactgct
     6481 ccataatcga ttactttaaa cttgctattt ttgtattcaa ttataccata tccaactatg
     6541 gctatacctg ggtcaatccc caatattatc attttttaaa attcccccta aaaataaaag
     6601 aaataccaaa atgtatttct cataaaaact tatgttctaa atcattatat cataaataat
     6661 attaacaaat cataaaatgc tctatgtaaa atataaaatc aaataaaaaa gttatatcaa
     6721 agcaagattt tacctcaata taacttttat atttattaca aataaattat tgtattttta
     6781 taggaattaa atgtgactac aattttaata ctaatctatt taatattaaa ttatttatta
     6841 ttaatttact tagtatttaa cttacttagt atttaactta aaataatatt aatttactat
     6901 taacttgcaa agtcttctaa tcttgaatag gcctcttctt cagtatccat aactacacct
     6961 tttttgattt catcttgaac tgttttagga agtgaatcta ctcttatttt tgttttttct
     7021 actaatgttt tacttccgct atcatcatat ttatatagtg ctatatttcc attatcattt
     7081 tcaatagtat atttaccttt tatagtaaca tccattgctt gtacatcttt attatcacta
     7141 tatttagatt ctaccaatat gatttcattt ttatccatac tctttatttt tttagttgga
     7201 tattcttttt taaaatatga tactatttct gacttcgtct tatctaataa tgactctgga
     7261 actattcctg ccattgtaag tgttctctta tagttagttt cattttcgcc ttcactttga
     7321 agccaaactt cacaattttt atttaatcca aaagcttctt ttgacttttc attcatattc
     7381 gcactagtac ttacaacttc accagtttga actccattgt gttttaatct tgtccctata
     7441 taaactccgc tacttaatac aaatactcca accagtatta aggcagatat tttccaagga
     7501 aataattctt ttttcttctt ttcaaacatt gattacgcct cctttttcta ttattttctc
     7561 caagctgtaa gtttttatac aatttaggta aattttagaa gaaaatatat ttatatttca
     7621 aattactcta agaatagata ttttctaaaa ggtagttttc tcttaatggt tatattcttg
     7681 taattatatg gacataattt ataataaata atcgtttagg aggtaataac tttggaaaga
     7741 ataaactgta atgtaattaa ctgttcacat aacaacagcc atatatgtta tgcagataaa
     7801 atttttataa atggtgaaaa agctcacaat gacagacata cttgttgtag ttcattttta
     7861 gatgaatcta tttatagtaa tttatctagc aacgttaata atagaaattg tgaggaatta
     7921 gtttgtactg taaaaaattg taaatacaac aaagatggta gcttatgtac acttgataat
     7981 atattggttg ctcctgaaac tgatagagta aacctttata ttgaaacatg ttgctctagt
     8041 ttcgaatgta aatagtcata gtataaatat aagggtgtca aatgtaatta aactaacata
     8101 gtgtagagta tctacaacta ttagtttgat tatacttgac acccttattt attttaaata
     8161 aaatttcatt attataacca tgtttataat gttctaactg taattaaatt tgataaattt
     8221 ctataaattg gtataatcaa tatgtaaatt gaatttagta aattaatcaa aaaaataaaa
     8281 tatcgagtat atagtgttat actaactaga aatgaggtgc ttatgagtac attttatatt
     8341 caatttcttt attttgtaat ttatagcttt ataggatggt gttgtgaaac tacatattgt
     8401 tcagttttac aaaaacaatt tgtaaataga ggttttttaa atggtccatt ttgccctata
     8461 tatggatttg gagcattagc aatagttgct accttgactc cttttgtaca caatattcct
     8521 ttgttatttt tgtttagtat aattataact agcataatgg agtattgtac gagctttatt
     8581 ttagaaaaaa tatttaatat gacttggtgg gattattcta aacataaatt taatattcat
     8641 ggaagagttt gtctggaaaa ctcacttatg tttggtgtat tatcattgat tgtaatgctt
     8701 atagtacatc ctattgtggt tgactttatt aactctatct ccaaaaatgt gctatttata
     8761 tttgctatat ctatagaaat ttattttgtt ttagatttag ttatcacagt ccatacaatt
     8821 ttacaattaa atggtaagtt aaaacaaatc aatcttataa ttaaggaatt aaaagataag
     8881 aaagaatact acaaacttat tacacaagaa actatagaaa acaaacttga atctttagtt
     8941 gaaaatagac tggatacttt ggaagataga tttgaaatac ttaaggaaaa agaaaactat
     9001 cgctatgcta aaaatcgtat tgatgagcta aaaaataaac ttaatacttt gctgtctaac
     9061 cacaaattac ttcatagacg tataatcaaa gcattcccca acataagctc taataagcat
     9121 acagatattt taaataaaat caaagaaaat attaaatcaa ttaaaaaaga taaataataa
     9181 aataaacaca taagttaaat aataaaaata ctaatacata agattaaggt ttagaatatt
     9241 ttttgtaaaa tactctaaac cctaattaat atactattat ttttaatctt aatttataat
     9301 tttagtttat tttactttta acacagtaaa tttaagcgtt taaacttcaa tacactaata
     9361 tactacccca tcaatatctc aactgattct ctaagtgtgc taatctctcc cacatttcca
     9421 gggaaaatta tatatggcat attaggaaat ttgctttctt tgcctgtcat ccatactggt
     9481 ataccaggtt ttatctgtcc cattactgta gcttttttta ctcttagtgc tttagtacca
     9541 acatcactag aagtaattcc acccttagct ataataaaat ttggccttac attaagcata
     9601 ccaattatac tggtaactgc atctgatatt tctacagaaa tcataagctg tttatcttta
     9661 tcatttgtat ccaagtcaaa acgctctctt ctagtataaa cagcaacagt tttacccctg
     9721 cttatttttt cctctacaac ttttataact ctttttacct cattttttag accattttct
     9781 tgaagaacta aatgttgatt aaattctata aattctattg gatatttaca atttttaagt
     9841 tcttctaact gttgggtagt cttattcaca tgtgaaccaa caagtataat tcctccattg
     9901 gtattacctt ttgatactaa ttcatcctta gtaagcagtt ccttatcact cactccgcca
     9961 agtacttttg ttatggcagc agcgcttcta aacataaatt ccttaccact atttactgct
    10021 cgtataaacg caattgtaaa tacctttaca tctacatagt cggttgcatt tacaataatt
    10081 ttattgaatc cttcagcttt tttaagtttt tctgttatag cttcaatatt tagctctcta
    10141 agttcttttg acgaaatata tatcatatca ctagatttgt actttccttt tgtcttttct
    10201 tcacaccact cacctatatc agatgattta tatccaaaag atttatcttt tgcaaattca
    10261 gtcattcctg ctggtacaag tatttctcct tctttaacat aatgtacatt atctatagta
    10321 aacctaccac cttctttaaa aaatggcatt attatttcac cctgaaataa cttccccgat
    10381 aatctctcta cttcgctttt taataaatta gtttcaagag gataatgtcc tctcatagta
    10441 gaatcactac gacttatgat tataaaatcc ttattaactt tctttgatac atccacaata
    10501 ttctttgaaa tttctttatg tgcttttgta gtttgagcaa cagtaaatcc ccttgaattg
    10561 gtaagtataa aaaacatact atttttctca ttaaaacctt gttccatact atccttatcc
    10621 caatcagtat aaacagaaat atcatgaact gtttgtacac ctgttgggtc atcatctaat
    10681 acaacaattt ttctatctaa atgcttaagc tcatctgcaa gaagtttatt taatctgtcc
    10741 tcatctactt gtttaaaagt attaagtata tctatgctaa ttggttttac ttctctcatt
    10801 taaaacgccc ctttatgtaa tttgtatagt ctaaatgtaa tttatatgat ataaacaata
    10861 gtttgttatt tatagacctg gaagtggtat aattccattt ataacactaa gcaataaaac
    10921 cattataaat gctacaactc cagcaatggt tcctccaatt gtccaagagt ttaaagtatc
    10981 tttaactgat aatccactaa atcttgaaag tacccagaat ccagaatcat ttggaaggga
    11041 gaaaccaact cctccagcac atatagcaag ccctactaaa actggtgaca ttccagttgc
    11101 caaaattgta ggtcctaaaa ttgatgatgt tgttactaag gctacagtag cagaaccttg
    11161 agaaattcta agcaaggcac ttaataagaa tccaagcact acaactgata tactaagacc
    11221 agacatagta tcaactaaga agttacctat accacttgca tttataacac tgccaaacgc
    11281 accacctgaa cctgttatca ataatattag ccctgctgaa tcagcagctt caataactac
    11341 atctcccata ggtttattta tgtatttctt taaaaatcct attgccacca atattccaag
    11401 aagtattgct atgtttttat ctcctataaa tgcaagtact ccactcattg tagaaccttt
    11461 ttctaagaac atactaaata catttgatat aagtataaga agtattggga acaataaaga
    11521 aaacatagaa attccaaaac ctggtacttt agaagaatca tcatcttcaa tttttatgct
    11581 atcttttccc tgctctaaat ttttttctct atcattctca tcaatagcat aagctggata
    11641 tttttcaaaa cgttttccat aaatccatcc acctataaga gctgctggaa aagcaacaat
    11701 tatagaataa agtataaaac tacctacatt tgcatttaca tttgctgcaa ctgctaaagg
    11761 tcctggtgta ggtattacaa gtgcatgacc tactatagtc ccaactccta atgcacatac
    11821 aaaaaccatt agagatttct tagttactct cgaaacatac ttaattatag gcatcattat
    11881 aacaaaagct gcatccataa aaactggaat tgaaattaaa aaacctgctg tagttactgc
    11941 taaagcactg tttttttctc cagtcttagc taacaacccc tttgcaatag attctgttgc
    12001 tcttgattct gaaagaatat ttccaaatat tattccaagt ccaattacaa tacctatact
    12061 acctaatgta tttccaaatc cagtcgatat tgtggttgat atttcattta taggcattct
    12121 aaccataaag cctgtaatta tagatgttat cagtaatgct aaaaacggat ttactttaaa
    12181 tttaataatc gatacaagta aaattcctat agatataaca aatgctatca aaagtccata
    12241 tcctgtaatc ataatcttta cccctttatc attctacttt attatttatc aattttattt
    12301 ttcttttact atgataactt aacctcgctg tatttatata atctaatcct aataatatgt
    12361 atttaattat attgatatat ttaaatatta attatcgtgt ttttttattt caacacctgc
    12421 caacttttca aaatactgtg ctattccact atggtcatct tccaaatgtc catgtacttt
    12481 taatgtctgc ataatttcta ataattgact tgtcataggt agcggtacat ctaaattgtg
    12541 tgctgtttgc attacattct taatatcttt caaattaata gatatttttc cacctggttt
    12601 aaaatcacga ttcataatca ttggtatctt cgcatccaat attgtactac cagctaggcc
    12661 tcctcttatt gccttatata ctttttctgg gtctgcacct gcttttgctg caagtacaaa
    12721 tgcctcagaa actgctgcta tcgtcaaatt aactattact tggtttgtaa gttttgtaac
    12781 acttccacta ccattatctc caattaaaat agatgagctt cccattatat taaaaaatgg
    12841 ctctactttc ttataaattt cttccttacc accagccata aaagcaagtg taccatcaat
    12901 cgcttttggc tcccctccac ttactggtga gtctataaat tctaagccca tatcttttaa
    12961 tttatttgca cacaatattg attctgtagg agtaactgaa ctcatatcga ctactatact
    13021 tccttctttt aaagtttttg ccaatccatc aatcccaaac aatatatcct gcacaatagt
    13081 tccatttgga aggattgtaa atacaatatc acattcttga cccatttcta aaacacttgc
    13141 gtattttgct ccacatgata taagttcatc cacagcagat ttattaatgt catacaccaa
    13201 aagattacat ccatctttta ataaattttt tgccattggc tttcccatta ttcctaatcc
    13261 aataaatccc aatttcattt tataaccctc catatcttca catttttgaa aacgtttttt
    13321 atgttttaga gtatactaca taccagtata ccggtcaata taaaatataa aagtatttat
    13381 tttaacaata aatagtaatt tttaaagtca tttattatat ttagtcatcc tatataagtt
    13441 ttaatacata ctattgtttt caaatataat atactacttt tacagttttg tattttaact
    13501 aatttcaatt agttttaatt aatctgcctt taatgttaat catgcttcac acttcaataa
    13561 ttttttatat tttttaatct tatctgcaat ttaaattgca aaagattgac cttattttca
    13621 ctatgttgta tgcttaaaat tatataagtt tttgtatttt tatattgttt aggggggtat
    13681 tattaagtat ggctacattt aaaagattac aagaacaagc atatgactac ttaaaagaat
    13741 tgatactatc tggaaaaatg atagaaaatg aaatatattc tgaaacaaaa cttgctagtg
    13801 aaataggtat ttctagaaca cctataagag atgctcttca aagacttagt caagatggat
    13861 ttatagatat aatcccaagc aaaggattta gaatccatca aattacagca aatgaaattg
    13921 tagagatatt ccaaatacgc tctgcaatag aaggattttg tactttttta attacaagtc
    13981 aatacaaaga agctagagca gtagaaacta taagtaaatt aaaacatctt ttagataaac
    14041 aaaaagatat tttattaggt gataaaaatt taaactcatt tgcagaatat gatacattat
    14101 ttcacacaac tatagtttca tatgctcaaa atactgaatt tgacaaaatg tttaacaatt
    14161 atatgtacag aataaaaaaa ttagctctcg attcattgtc acatgaagga aggcttgaaa
    14221 caacattaaa agagcacaca gatattttta ataatattgc aaatggatgt attgaagata
    14281 tttacagaac tactctcatt catatggaaa ctcctaaata cattaattta gaagattttt
    14341 gtaacaataa gttattttag cattatacct tcatttatta aaaatatatc tatctcaaat
    14401 taatttccta atttataaaa aatattctat aattatattg taactaatac ttattctaag
    14461 gagtgatttt tttgaacgaa gatgttgtaa ctaatgaaac tattgacaaa gattatgcta
    14521 ttgaagaagt tcgtatggca tgcaaacatt ttggtgattt atatttttat ttttccaaag
    14581 tgttatttga agagtttggt gaagataaaa ctagcgaaat attaagaaaa gttttatttg
    14641 aaaggtctga agaaagagct attgctatga gagagagagc tcatgaaaat ggagatgaat
    14701 taatcgcaga caatattatt agcacaactg atgttccatt tctaggatgg gttcctgaat
    14761 ttgaagagtt acattgcccc tatggagctt cctggctttt aagatttgag gaaaatcctt
    14821 ggtttaaaaa atttgcttct ctttactgtg atgttacaga tactactgta gctgaagtat
    14881 ttacaggaga tacttcccat aaaataacaa aaaacattct atggggagat aaaagttgtg
    14941 aaagaatata tttccatgat gacaaggtat ctgaaggtaa atatacttat ggaaataaaa
    15001 actgtaaata aataatttaa attttctata ttcatatact atattttgta ctt
//
LOCUS       DAEOSP010000090        15049 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.090, whole genome shotgun sequence.
ACCESSION   DAEOSP010000090 DAEOSP010000000
VERSION     DAEOSP010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15049
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.090"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            206..784
                     /locus_tag="KRH54_002120"
     CDS             206..784
                     /locus_tag="KRH54_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG8579777.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            823..1401
                     /locus_tag="KRH54_002121"
     CDS             823..1401
                     /locus_tag="KRH54_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG8579778.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1487..2116
                     /locus_tag="KRH54_002122"
     CDS             1487..2116
                     /locus_tag="KRH54_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG8579779.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2106..4865
                     /locus_tag="KRH54_002123"
     CDS             2106..4865
                     /locus_tag="KRH54_002123"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBG8579780.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     LKKTA"
     gene            4952..6514
                     /locus_tag="KRH54_002124"
     CDS             4952..6514
                     /locus_tag="KRH54_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBG8579781.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6515..7651
                     /locus_tag="KRH54_002125"
     CDS             6515..7651
                     /locus_tag="KRH54_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG8579782.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7715..8791
                     /locus_tag="KRH54_002126"
     CDS             7715..8791
                     /locus_tag="KRH54_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBG8579783.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDFMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8769..10007
                     /locus_tag="KRH54_002127"
     CDS             8769..10007
                     /locus_tag="KRH54_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HBG8579784.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10061..11149
                     /locus_tag="KRH54_002128"
     CDS             10061..11149
                     /locus_tag="KRH54_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBG8579785.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            11177..11968
                     /locus_tag="KRH54_002129"
     CDS             11177..11968
                     /locus_tag="KRH54_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBG8579786.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYTNIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKCDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(12083..12637)
                     /locus_tag="KRH54_002130"
     CDS             complement(12083..12637)
                     /locus_tag="KRH54_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG8579787.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            12833..14035
                     /locus_tag="KRH54_002131"
     CDS             12833..14035
                     /locus_tag="KRH54_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBG8579788.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            14092..>15049
                     /locus_tag="KRH54_002132"
     CDS             14092..>15049
                     /locus_tag="KRH54_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBG8579789.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLSEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYR"
BASE COUNT         5925 a         1552 c         2741 g         4831 t
ORIGIN      
        1 tattaaacat agcttttata catttaataa ggtatgtaaa atctatgtaa aaagtaagaa
       61 atataaacat ttttttaatt atattttaaa aatatcaagc aagttttctt cacaaatgaa
      121 taaaaaatgt tataatatga ttaaagaaaa agataaataa ttagcaagat ttgtttagct
      181 aaaattaaca agggagcgat aaattatggg tataacatta tcaaaaggcc aaaaggttga
      241 tttaactaaa ggaaatccag gtcttaaaaa tattttagta ggtttaggat gggatactaa
      301 caagtatgat ggtggatttg actttgattt agatacagca gcatttttaa caggagcaag
      361 tggaagtgtt actaatgatg gagattttgt attttacaac aatttaaaac atacatctgg
      421 agcagttgag catctaggag ataacagaac tggcgaaggt gatggagatg atgaacaaat
      481 agttgttgat ttatctaaga ttcctggaga aataagcaag atttctttta ctgttacaat
      541 tcatgatgca acagaaagaa gacaaaattt tggacaagta agtaattctt atataagaat
      601 agtagataaa gacacaaatg aagagttaat aaagtatgaa ttaggagaag attttagtat
      661 agaaacagct atagtagtag ctgaaatata taagcacaat ggagaatgga agtttaatgc
      721 actaggttct ggctttgaag gtggtcttgc tgcattatgt ggaaactttg gaataaactt
      781 ataatttcaa ctataacaat aaactaaagg gggatatttt ccatggctat agtattaaaa
      841 aaaggacaaa aaatcgattt aactaaggga aatccaggtc ttaaaaatat aaaacttgga
      901 ctaggatggg atacaaattc atttgatagt ggctatgatt atgatttaga tgtaagtgtt
      961 ttcatggttg gagaatctca aagagttgaa aaagatgaag attttatatt ctacaataac
     1021 ttaaaacatc catctggagc agttgaacac ttaggtgata atagaactgg tgaaggtgat
     1081 ggagatgatg aggaaatatt agtgaattta gagacaatgc caaagcatat acaaaagata
     1141 gctgtaacag ttacaatata tgaggctgct gaaagaagac aaaattttgg acaagtaagt
     1201 aactcatata taagagtatt aaattctgaa aatgatgaag aaatattaag atatgactta
     1261 ggtgaagaat ttagtataga aactgcaatt acagtatgtg agatatataa gtataatgga
     1321 gaatggaagt ttagtgcagt tggagcagga tttgaaggtg gcttagaagc actttgtaga
     1381 aactatggct taaatgttta atttaagcaa tataatatat taaatataaa taacctatac
     1441 aaataatgta taggttatat tatataaaat tttgtggagg tttagtatgt cgattaattt
     1501 atcaaaaggt gataaaatag atttaaaaaa atctaaccca ggactgagta acatacttgt
     1561 aggtctagga tgggaccctg tgcaacaatc tggtggaggt ttttttaaat cgttatttgg
     1621 tggaggacaa gctgatatag attgtgatgc atctgtattt atgcttaatc aagaaggtaa
     1681 attaagtgga ataaaagatt taatttattt tggaaatcta aaaagtgcct gtaaatcagt
     1741 tttgcataca ggagacaatc taactggtga gggtacaggt gatgatgaac aaatcctagt
     1801 taatttggat aaagttccat caaatattca taaattattg tttgtagtaa atatatacaa
     1861 ttgtgtagat agaaaacaac actttggaat gatagaaaat gcctatataa gagttgaaga
     1921 ccaaggtaat aagaaagaga tagcaaaata caatctatct gataattatt ctgaaaaaac
     1981 tactcttata gtaggagcta tatatagaaa agatggaagt tggcaattta aagcaattgg
     2041 tgaaggaaca aaagatgcgg gtcttaaaga agtaatgcaa aatttagaca ggattgagtg
     2101 tgcatatgga atttaataat gatatcaaat attataatgt agataaagaa gaagttacaa
     2161 aagacttatc tgttaatcct gaaaaaggtt tatcagaatc agaagtcaag actagaagag
     2221 aaaaatatgg tttgaatgaa tttactccaa aagaagaggg tagtttttgg gatgatttaa
     2281 aggaaagttt aagtgaacca atgatagtta tcttgattat tgcagcagta gtaagtgcag
     2341 taattgggga aactcatgat gcaataggga ttgtaggtgc tatagcaata ggtatagcaa
     2401 taggtatgat tacagaaggt cgttctaaga aagcagcaga ggctctttct aagttgactg
     2461 aaaacataga agtaaaagta ttgagagatg gggaagtaca tcaaatttct aagagtgaat
     2521 tggtaccagg tgatatagta tatattgaaa ctggtgacat ggtacctgct gatggtagac
     2581 ttatagaaag tataaacctt aaaattagag aagatatgtt aacaggagaa tctgatgacg
     2641 tatctaaaaa gtgtgatgta gtagtttcta tggaagagat agaatctaaa ggagcagtag
     2701 tagtacagga gccaatacct gcaaaacaga taaatatggt gtttggtggc acacttgtag
     2761 cttatggtag aggagctcta gttgtaactt caattggaga taatagtgaa atgggtaaaa
     2821 ttgctcaaaa cttatcagaa acagatgagc aaactccact tcagataaaa cttggaaatt
     2881 taggtggttt gatagccaaa gtttctagtg cagtagcagg attactattt atatttatgg
     2941 ttttccaaat gataagtaag aatgtattaa atgtagatat gtctggaata ttaccgtttt
     3001 tagaatcaat agacccagtt aaaacagcat ttacagtatg tgttgcgttg atagttgcag
     3061 cagtgccaga aggtcttcca actatgataa atatgacact tgcaataaca atgcaaaaaa
     3121 tggctaaaat aaatgcattg gttactaaaa aagaagcttg tgaaacgata ggttctgttt
     3181 ctgtaatatg ttcagataaa actggtacat taactgaaaa tagaatgact gtagaagttg
     3241 catatgttga tggcagatat attgaaagtt cagaagagga aataaatagt tattttgaag
     3301 aaaactgtat gataaactct acagctgatg tagagcacaa tgatggtgat attaagtatt
     3361 taggaagtgc tactgaatgt gcattgcttt tatattacaa aaacgtagat tatagacaag
     3421 ttagaaaaaa ttctaatata gtagctcaaa atccttttac ttcagattca aagagaatga
     3481 cttctgttat aggtcaagat aatcatcatg tactattatc aaaaggagca ccagaagtct
     3541 tacttgaatt atgttcacat attcaaagag gaaaagacat agttccatta actgaaaata
     3601 taaaacatga gattttagaa gaaataaaga aattgcaaat aaaatcaatg agaactttag
     3661 ggtttgcata taaagaaatc tcacaagctg aagaagaagc agcagtaact gctgaaagtg
     3721 atgctatgaa tgtaagtgca atggaaaaca atttagtatt tagtggtttt gttggtataa
     3781 gagacccact tagaaaagat gttatagagt ctgtaaatac agcaaataaa gctggtgttt
     3841 ctgtaaaaat gcttacagga gataatataa atacggcaag agctataggt gaagagttag
     3901 gcctattaaa aaataatatg agagtggttg aagcttcata tatagatact ttagatgatg
     3961 aagaattaaa acaagagata caaagtattt caatcgtggc aagaagtaaa ccagatagta
     4021 aaatgagaat agtttcagct ctgcaaaaga gcggagatgt tgttgcagta acaggtgatg
     4081 gtataaatga tgctccagca ctttcaaaag cagatgttgg tatagcaatg ggtatatctg
     4141 gtacagaggt atctaaaaac gcagcagaca taattttaac tgatgatagt tttagtacca
     4201 ttgtaaaagg tattaaatgg ggtagaggaa tttatgataa cttccaaaga ttcgttcaat
     4261 tccaattaac tgtaaatgta attgcatttt tagtggctat aatatctcaa gttatgggac
     4321 aagagatgcc atttactaca atacaattat tatgggtaaa catcattatg gatggaccac
     4381 cagcactagc attaggttta gagccagtta gggaccatgt gttaaataga aaacctgttg
     4441 atagaaaagc caatataatt tctaagtcaa tggtgtatac aatagtatta aatgcatttt
     4501 atataacagc aatactaatg ttacaatcta catttaattt cttaggagtt gatgaagcgc
     4561 ataagggaac agtaatgttt gcgttatttg cttttagtgc actgtttaat gcatttaact
     4621 gtagagagtt taatgcagat agtatcttcc ctaattttac acataacaaa ttagcactac
     4681 aaataatagt gttgacagga attgcacaag taatatttac tcagctattc caagatttct
     4741 ttaactctgt agcaatggat tttatgacat gggtaaaagt tttatgtgta gcagcaagtg
     4801 taattgtggt taatgaagtt gtaaaatgta taataagact ggtaaaacta aaaaagactg
     4861 cttaaaacaa attttttaga agaaaacaga ctgactcaaa atatgaattt tgggtcagtt
     4921 ctgaataaac atttaaattg ggaggatagt tatgggtgac tcaaaggtat ttgaaaaaat
     4981 attctccagc caatcagata gaggaaatta cactccttca aagggatatc taagttattt
     5041 tatttcgtat attggtttag aagatgaagt tttgtacaat ctcgagatat ttaagacaaa
     5101 acaaaatatt gatagcaaaa aagatattgc tttatttact gatgttatag caaatcctag
     5161 tgattttgat attataaatt attttaaaag tggacttcaa aaatatagaa ctagcatgga
     5221 agatgtagat ataaatattt tgggatttga agagattgat tataaaataa agcaagcaat
     5281 ggatagagtg ttaaaagaag aggaaaaaga atttactaat gatagagtta aacaaaattt
     5341 tatagtgaaa ataatggcat ggataaaaat ttatatcggt gctttagata taaataaaaa
     5401 tgaagctcca aaagtaatat tttatggaga tataaaaaaa catgaagttt atttactctt
     5461 aatattatac ttggcaggtt ttgatgtttt atatcttaat ccaaattcaa agagtaatat
     5521 agatatttta aaatcagaaa gatacaatat agagtttgaa gaagccaaca taatagaaga
     5581 aaaaatttct tttgaagaaa gggttatttt aggggaaaaa atagataaaa gttctgtaaa
     5641 aaaagctttt actgttgggg cagaagcatc aaagcgtata agtgaggaat tgttaaatga
     5701 tgcaggattt ataaaacctt ggcaactcca agatagaaaa ataaaaaatc tacttctaag
     5761 ttcaactgtt gatgaaatat caatatactg gaatcaacca cttaaattaa gaccaggatt
     5821 taagtttaat gatgctattg tagaagctcc aaattttctt tctaagatta atggaatata
     5881 taacgataag aatgaatata ttaagttttt agatttatta agagattcgg aaagttcaac
     5941 atttattgaa tttaatggag atgtagatag attttcaaaa gcttttacaa gagaagcttt
     6001 tagtttatcc tttttattgg attcaaaagg tgcaatagat aaaaactctg ttttaaataa
     6061 taaagattat tccatatcaa cactagcatt gaatcaacaa attatgattt tagagaaagt
     6121 ggaagagttg ttagaaggaa gtatgttttt gaatggttta agtggagaag acaagataaa
     6181 aggtctattt acagtacttc acatggataa gaaatttgtt catatgatga acaatttcga
     6241 ttactcactt ataaacccaa aattgattat atacatgtac aagagtattg tatttgataa
     6301 agaaatagtt tttcttatgc ttttacttag taagattgga tttgatatta tcatattatg
     6361 tccaggagga gaaaacaata tagagaatgt tataaataac caattgattg atgtacacag
     6421 attggataaa atggtatatg accttaaatt aaattcgctt gaaaatgata tacctttact
     6481 taaaaaaatt tttggaaaga ggagacgatt ttagatgggg ataaatataa atagtttttc
     6541 aaaagataag aactctgtag aggttactga aaatttgcct acagcagttc aagaagaaaa
     6601 cttcgatata atggaatata caaataataa aaaaaatgaa ctgagaaagt ctaaggaagt
     6661 tgaggcattg acatctctta tagaagtaga aaatccagat acgattttac agtttggtag
     6721 gaaagcatct gaaggagtag caagagtttc tgatagcctt ttaaatacaa taaaattaaa
     6781 tagaaatgaa gaaaattcta aaatgttggt tcatttaact aaaataatgg acaagtttga
     6841 tttagacgat tttcaagaaa ctaaggaacc aaatttcgtt caaaaacttt tcaaaaaagc
     6901 taataatgcc attgaaatga tgtttcaaaa gtatgaaaca ttgggaggag aagttgagaa
     6961 aattcaaatt gagcttgaac aatacgaaag agatatagca ctttctaata agcaaatagg
     7021 tgctatgtta aatgaaaact ttgagtttta caatgaactt caaaaatata ttgttgctgg
     7081 agaaatggca atagaagaaa tggataatga aatactacct ttctttaaac aaaaatcaga
     7141 gactagtgga gaccaaatgg atgttgtcaa ttatcaagaa ctattaaaag tttatgatat
     7201 gttaaatcaa agagtctatg atttaaggat agcagaaaat atagctatcc aaacaatacc
     7261 tatgcttaga ggtatgcaac ataataatta tggtttaatt agaaaaataa atagtgcatt
     7321 tgtcgtaact ttaccagtat ttaaacaatg tttatctcaa gctatattac ttaaaaagca
     7381 agaattacaa gcaaaatctc taaaagcact tgatgacaaa actaatgaat tattgcttag
     7441 aaatgcacaa aatgtaagta ctcaaagtgc acaaatagct agaatggctg gaacaagttc
     7501 tgttcaaata gaaacattgg aaaaaatgta caatactatc aagtctggta tagatgaaac
     7561 tatgagaata gaagaaaaca atagagcttt aattaaggat aatacaaaga gattggaaga
     7621 attaaatacg actataattt ataataagta gtattatatt aagggctaat ttgtataaag
     7681 ttggcccttt ctatgtatat gaggaggcta gacattgaaa tatttcgatt atatctgcaa
     7741 agatgatttg gagagaattt ttttgaagga gccagaagat tttagtgcta agactgaaaa
     7801 agatgtgttg aaatatgcac tgggcgcttt tttatacgtt ccagctactc aatataatat
     7861 gatttataag agtattatag gggatgttaa gggagttaga ccattagcta tttgtttaga
     7921 agatgctgtt ggagtaaatg gagagttgga ggctatagaa aatcttagat taattttaaa
     7981 aaatattagc aatgaaagta taactaataa agatggaata ccactaattt ttgtaagaat
     8041 aaaagatgtg gagcaactgt tgagaattaa agagattata attaaaaata gtcattttat
     8101 aacaggaata ttaattccta aagcaaattc ggagttaatt gaaaattgta tagaagcttt
     8161 agattttatg aatcttcaag atatgtatgt tattccgata gtagagacaa aagaatttat
     8221 atataatgaa aaaaaagaac ttagttttac taatttgtac aatgctattt taagacataa
     8281 gtcaagaata ttaagcatta gagtaggcat aactgatata ttagggatgt atggtataag
     8341 gagagataaa aacttttcta tatataataa tttgatttgt tcttcattta tattagatat
     8401 aatcacatat cttcaaagac cagagttaga tatacctata agtggtggag tatcagaatt
     8461 ttttgatatg acgaataagg atataagaaa taagtatata gaagaaatat tattggataa
     8521 atatcatgga ttaattggaa agaccgtaat tcatccaatg caaattcaaa ttgtacaagc
     8581 gctatctaca gtatcatatg aagattttac tgatgctttg gatatactgg atagtacaga
     8641 cagtaagtat ggtgtgagca agggtgtatt aggagagaga atgaatgaaa caaatccaca
     8701 ttttttatgg gctaaaaaaa cattgattct atctaaaata tatggtgtat taaataaagg
     8761 agtagactat gaagaattgc ttaaatttta attgtgagat tgacattatt gataatccat
     8821 tgaatttaga actaaatgat tttttagacg taggaataag aaataatagc aaaagacgtt
     8881 ttttgtttat ctcaaagaaa ctcggtaaac atttagcatg taagccaagt gagatggata
     8941 atttgggaaa attaatggct acaatttata atgaaaaaaa taaggaagaa tcaaatggta
     9001 ctgttatagc ttttgctgaa actggtacag ctgttgggca tagttttttt gattacttat
     9061 gtggagatta tgaatttatt catactacta gagagcagtt tgacgagtta gaaaatctgg
     9121 aatttttaga ggaacattcc catgcaacag accagaattt atactttggt atgcttaaca
     9181 actttaaatt tggagatgag ataatattgg ttgatgatga gattaccaca gccaatacat
     9241 gcatgaatat aattaggaaa attcaaagca tttatcctaa aaagaaatac actatatgct
     9301 caatactcaa ttgggtaagt gatgaaaatt taagtaaagt tactcgttta gaaagagagc
     9361 tagattgtaa gatagatttt gtatacttat ttaaaggaaa ctttaaattt tatatagatg
     9421 aagatattat ttcaaattac aacgataatt atagtattac taaattagag actatttcaa
     9481 gtgataaagt aaatttagta gtaaatcata tttatgttga tttagaagat tatattggaa
     9541 ataagaaata tgtaaaatat acaggaagat ttggaattaa tagaagagaa caatttagat
     9601 taaaagaaat cgttaagaga gaaagtaaaa aaataactat ggccagtaaa gaatctttaa
     9661 aaaatgaaaa agaaaagatt ctatttttag gtactgaaga atttatgtat atacctatgc
     9721 tgtttgcaaa agaaaaagaa aatcaatatg atatatacta tcattcaaca acaagaagtc
     9781 caattattaa tattgataag aataattatc ctattaaaag taaatttatg ctaaagagtt
     9841 tttataatat agatattcaa aattatattt acaatataga taaacataat ttttctaagt
     9901 gttatttatt tgtagagctg aataaaagtg aaaattctta tctagaattt attgatataa
     9961 ttaaaaaaac aagtataaaa gaattaacta tagtatgttg tagctaaagt gttttaacag
    10021 gataaaatat atcacgattt taagatagga gttgagtcat atgagagatt ttagcacgta
    10081 ttctaaagat gatgttattt ttttattaaa agatatatca aatttaattg aagaagaagg
    10141 aaataaagaa agagaattaa aaatacaaaa tggtagacat tattcagaaa tgataccaat
    10201 agagtatgaa gttagtgaag attatctgaa tttatatcat gagaaactaa gagaaaataa
    10261 agataaactt gcattttcta taggagttat gtgtgaaaaa ataattaaaa aaaatggaaa
    10321 tgatgtagta ctagtttcac ttgctagagc aggaacacct attggtattt tggcaaaaag
    10381 atatataaga agcaagtata atataagttt acctcactat acaatctcta taataagaga
    10441 taagggaata gatatgaatg caattgaata tatagtaaag aatcatccga gctcaaaaat
    10501 tcaattttta gatggatgga ctggtaaggg aactatatca aaagaacttg aaaaagcatg
    10561 tgatgaactt aatgttaaat ttaaaaaata ttttgactca acacttgctg tattagctga
    10621 ccctgctgga tattcaggac tttatggaat tagagaagat ttcttaatac caagtgcatg
    10681 tttaaattca acagtttctg gtttagtaag tagaactgta ctaagagagg atttaattgg
    10741 taaaaatgac ttccatggag ctaagtttta tagacattta aaagataaag atgaatctat
    10801 gaactatatc gaaactatag aagaatgttt taaaaatcaa tttaagaata tatctgatga
    10861 agttgaaaac tgggaaagtg acattataac aagagatgga tactttgatg ttttaaatat
    10921 aaaagaaaag tataacataa cagatattaa ctttatcaaa ccaggtgtag gggaaacgac
    10981 aagggtttta cttagaagag ttccgtataa gattcttgtt aaagacttaa atgataaatc
    11041 tttagaccat atattcatat tagctaagga gaaaaatgtt gaagttgaac aaatggattt
    11101 aaaagcctat aaatgttgtg gaataataaa aaatatgaaa gatatttagg aaatatatta
    11161 gacaggagaa tattgaatga ttgttttttc agatttagac aggagtatca tttactccaa
    11221 taaattttta aatgctgata gtaaatatac taatatagaa atttataggg aaaaagaaat
    11281 atcttatata tctttagata ctataaattt aataaagcaa atacaatatt atggtatgtt
    11341 tatacctaca actactagaa cagtggagca atttaagcgt atagaattta ataaatatgg
    11401 gatttatttt ccttggagta taacttcaaa tggaggagtt atacttaaag ataatgaaat
    11461 attgaaatca tggagtgaaa aaatagataa actaaaatgt gattatgagc ccattgaaag
    11521 tatgattcat aaatttaaag actatttgaa tgtagatgga ataactaatt ttaaagttgc
    11581 agaagataca tttttttata tagttgttga tttatcaaga tttaatttgg attcaatcaa
    11641 agaatataca aatatattgg aatcaaaaaa ttggaagttt tatgtgagtg gcagaaaaat
    11701 atattttata ccaaaagaaa tttcaaaaga aaatgctata aaatacttga ctaaagaatt
    11761 aggaatagaa tatttttatg cagttggaga ttctattatg gactatggta tgcttaatat
    11821 ctcaaataaa tcctatgtat tgaagcatgg tgatataaat aaaaatgaaa tagaaaattc
    11881 atttatatca agttctttca atggaatgag tggaactgaa gagatactat ctaacatttt
    11941 agacgagaac tgcctactta atatataatg actgatatat aaataataaa ctagaaaaat
    12001 ctgaaataaa ttatagtaaa gtttatcata ctaatttatt tcagattttt atcaatttat
    12061 atttaagtaa gaactcaata tgttaaaatg aaggtggagt cattgcccag actgagatac
    12121 atacctcatc atcataattt tcatacacat gtggtatagt actgttaaaa taaaaactat
    12181 ctccttcctc caatagatat tccttatttc caagtgttat cttcatttta ccttttatta
    12241 tatagccaca ttcttctcct tcgtgtgtca caaattcact gtttttttgt gtatgaccat
    12301 ctagtgtaac aagtataaat tctatttttt tcccagataa attaggaaca agaagttcat
    12361 aaaattttgt agaatcatta gttactattt tttttcgatt atttttcttt actactatat
    12421 cagagtcttc tcttccaatc tcttcaaaaa aataacctat atttatatca agagcttttg
    12481 atattttcca cattgctaca atagatggaa caaccatatt acgctcaatt tgacttatta
    12541 agcctgtact taaatctgaa ttttttgcta aagtagagat acttatattt ttagattttc
    12601 taagagatct aattttgctt ccaacatcta tattcattag atacaaaccc cctcagacat
    12661 gattataaca tataatttat aaagattaat aaaataaaaa aaaagtttta caatacaagt
    12721 attttttatg ctatattgaa ttttctgaaa agatgcatta taatagtatt aatattcaat
    12781 atattgaata ttgataacga tattttaatt taatgaaatg gaggtaatta agatgaagag
    12841 ttttgtaagt cctgataata tttgggtatt atgggcagtt gtaactggat gggcagcatt
    12901 tagtatttat ttggaacaaa agtacaattg ggcatcaaaa gtgtcgggag caataatagc
    12961 tcttgtagga gcaatgttat tatcaaatct aaatattata ccagtagagt ctgttgtata
    13021 tgaccaagta tggggatatg ttgttccttt agccatagct ttattgttat atcaatgtaa
    13081 cattaaaaag atatggaaag aaagtggaag attgctcatt atatttcttg taggttctgt
    13141 aggaactgta cttggagcta tgattggatt tttagcatta aaaaatgtag taccagattt
    13201 aaatattgtt gcagctatga tgacaggttc ttatataggt ggaaatgtaa actttgctgc
    13261 tatgtcaggg gcgtttgatg cgccaggaga attagtatca gcaacagttg tagcagataa
    13321 tcttttaatg gcattatatt tctttgtctt aatagctata ccatcaattg gattttttag
    13381 aaaacatttt aaacatccac atgtagatga aatggaaagt ataggtataa ctgaaggaga
    13441 aacagtagca gcaaattatt ggggaagaaa agaaatatct ttaaaagata tagcaatagc
    13501 cattggtagc gcctttataa ttgttgcagt atctgtagaa ctatcaactt ggtttaaagc
    13561 tataatacca ttatcaaatc cttttttagc aatgttaaat actttatttg gaaatcaata
    13621 tctaataata actactttga ctatgttgtt tgccacattt atgcctaatt tctttggtga
    13681 tataaaagga gcacaggaat tgggtacatt tttaatatat atattctttg ttgtagtagg
    13741 tgttcctgct tcaataacat taataattca aaaatcacca ttactacttt tatactgcgg
    13801 aataatggta ctaataaaca tgctagttac atttatagtt gcaaaaatat ttaagtttag
    13861 tttagaagaa gctattcttg catctaatgc aaatattggt ggtcctacaa cagcagcagc
    13921 tatggcaata tcaaaaggat ggtctaagct agtagggcca atacttatag ttggaacatt
    13981 tggatacata gtcggtaatt attttggtct tttagtagga aatattttga tataatttta
    14041 cgtgaaaaaa agttgtttat tagaaagatt tttatttaat tggaggttgt tatgattttc
    14101 gatggacatt cagatacttg ggcagatgta acaataagaa ggcttaacgg ggaaaaagat
    14161 gtatttagaa gaatccatct tgataatttt aaaagagcaa atatagaagg agctattttt
    14221 gtaatatgga tagacccacc atatgataaa gagcctgaaa aaagagttaa acaaattata
    14281 agttctataa aagatgaaat tgaagatatg aaagatgttg taaacatagt aaaaaaatat
    14341 tcagatatag ataaaggtat aagtgaaaac aaaataaata ttttaattgg tcttgaagga
    14401 ttaagccata ttggaagaaa tatagattta ctagattatt tttataaaga agttggtgca
    14461 agacatgcat cattaacttg gaatgaacag aatgaacttg ctacaggatg tttaggggat
    14521 gaaaatcgtg gtttaagtga aataggcaaa aaagcagtca ataaaatgga aaaattgggc
    14581 atgatagtag atgtgtcaca tttaaatgaa aaatcatttt gggatttaat gtctgttgca
    14641 agtaagccag ttatagcttc tcattctaat gctaggagtt tatgtgatgc taaaagaaat
    14701 cttacagata atcaattaaa agaaatttca aatacaggtg gattagttgg aataaatagt
    14761 tttagaggat ttactaatag taatcctaag aagcaagatt taatacattt ggttgaccat
    14821 atagactata tggtaaatct tataggtata gaacatgtat cttttggttt tgattttagt
    14881 gattacttag ggaaagagac tataggttct tttactggaa atgctagtgt gccaagtgta
    14941 gatggtcttg aaagcacaca tacagcacag aacatagtag tagagcttga aaatagaggt
    15001 tataaaaaga aagatataga gttaatctct agtggtaatt tttataggc
//
LOCUS       DAEOSP010000091        15035 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.091, whole genome shotgun sequence.
ACCESSION   DAEOSP010000091 DAEOSP010000000
VERSION     DAEOSP010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15035
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.091"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            24..770
                     /locus_tag="KRH54_002133"
     CDS             24..770
                     /locus_tag="KRH54_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8579790.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            770..2686
                     /locus_tag="KRH54_002134"
     CDS             770..2686
                     /locus_tag="KRH54_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8579791.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNKNIKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICRKFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            2965..3882
                     /locus_tag="KRH54_002135"
     CDS             2965..3882
                     /locus_tag="KRH54_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG8579792.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQCNCDYESEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEISVVIKISHPFSTIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILLEIEKMKKIY"
     gene            3960..4697
                     /gene="cas6"
                     /locus_tag="KRH54_002136"
     CDS             3960..4697
                     /gene="cas6"
                     /locus_tag="KRH54_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBG8579793.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KDYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKVVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            4703..6580
                     /gene="cas8a1"
                     /locus_tag="KRH54_002137"
     CDS             4703..6580
                     /gene="cas8a1"
                     /locus_tag="KRH54_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HBG8579794.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNLIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQVMFNDYLRQIIYFGELEDLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKKYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTCINELFERNNSLKAQKSEYSDSKEENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFTDKYANKKIQSIYDYKLK
                     SNVLDLLLKNRDLKHLINNILRNKVKNDMESNNKSNISGIDCFRVVQLRALINSYKKG
                     VYKMDSKNLEKNDEKLRIIYYLGCDIHDYFVNKNSKNKIGGVSYKLLNSVKVGNKSDF
                     MDTIIRVFMSAEKQMPAFILDIEIEKDLDFESIGHAFISGLISGKYEGKDKLPNEKEE
                     K"
     gene            6582..7496
                     /gene="cas7i"
                     /locus_tag="KRH54_002138"
     CDS             6582..7496
                     /gene="cas7i"
                     /locus_tag="KRH54_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBG8579795.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGFADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGEIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFFSVDKFFGQLVKEVKEYYGVN"
     gene            7498..8199
                     /gene="cas5b"
                     /locus_tag="KRH54_002139"
     CDS             7498..8199
                     /gene="cas5b"
                     /locus_tag="KRH54_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HBG8579796.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            8210..10531
                     /locus_tag="KRH54_002140"
     CDS             8210..10531
                     /locus_tag="KRH54_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBG8579797.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDLVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQHEFKV
                     RYPIKTERLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIVGVGESNDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLAELTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFANSDRNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIYENKHTKQIFGKGVLISNEIDQFI"
     gene            10546..11097
                     /gene="cas4"
                     /locus_tag="KRH54_002141"
     CDS             10546..11097
                     /gene="cas4"
                     /locus_tag="KRH54_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HBG8579798.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEILIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKERIIENTLININEINMQLSP
                     SKIEKKHYCTKCAYYEFCFVKEE"
     gene            11100..12080
                     /gene="cas1b"
                     /locus_tag="KRH54_002142"
     CDS             11100..12080
                     /gene="cas1b"
                     /locus_tag="KRH54_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HBG8579799.1"
                     /translation="MYKDYYIISNGDIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNALMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIVDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            12083..12349
                     /gene="cas2"
                     /locus_tag="KRH54_002143"
     CDS             12083..12349
                     /gene="cas2"
                     /locus_tag="KRH54_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HBG8579800.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITEGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   12533..14334
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=12533..12561
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            14523..14951
                     /locus_tag="KRH54_002144"
     CDS             14523..14951
                     /locus_tag="KRH54_002144"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBG8579801.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
BASE COUNT         6235 a         1360 c         2515 g         4925 t
ORIGIN      
        1 aaattaggaa ggatagttaa ataatgaaaa ttttaactgt aaataattta agtaaagtat
       61 atggtaaaaa aataattttc aacgcattaa atgacataaa ttttagtata gaagatggag
      121 aatttgtagg aattatgggt ccatctggta gtggaaaaac tactctttta aatatgattt
      181 ctactattga caagccaact acaggaacta tggaattaaa gggcaagaat cctttattgt
      241 taagaggtga ggaacttgca ttatttagaa gaagagagtt aggttttgta tttcaggatt
      301 ttaatttatt ggatacatta actataggag aaaatatagt attgccattg acattagata
      361 aggtcagtgt aaaagagcaa gatgagagat tgaatgaagt atctacaata ttaggtataa
      421 aagatttatt aggaaaaaga acttttgaag tatcaggtgg tcaagctcag aggactgcta
      481 ttgctagagc attgattaac aatccatcaa tacttttagc agatgaacca acaggtaatt
      541 tagattcaaa gtcatcaaag gttgtaatgg agctatttca aaagataaat aaagaaaata
      601 aagttactac tatgatggta actcatgacc cattagcagc gagctactgt agtagaatct
      661 tattcataaa agatgggtct atatataatg aaatatataa gggtagtagt agagagcagt
      721 tttatcaaga gataatggat gtacttacat tattgggagg cgataactaa tgaatataaa
      781 acagtttgct attaacaata tttcaagaaa tattaaagcg tatttaggat acttagcaag
      841 tattgttata tcatcttcac tgctattttc ttttattatg tttacaagtc atccagattt
      901 agatgtatct caatttcctg attatttaaa agaaggtctc aagatgagta agataattgc
      961 atatctattc ttattcttct ttgtgtttta ttcagttagt gtgtttctta aaagtagata
     1021 taaagaattt ggaatacttt atattacagg tatatcaaaa cgccaagtaa tgaaattaat
     1081 ctttatagaa aatatgataa taaatatagt atcttctgta attggaataa taataggctt
     1141 aatctttgca aagatatttt tagtggttat gtctactttt ttagaattat caccattaaa
     1201 attctatata ccatttaatg cgatattgtc tactttaatc tattttatga ttttagctgt
     1261 attaacatca atatttactt cttttatagt taaagaaaat caggtgttga agttacttaa
     1321 aggtacaaaa actccaaaac cagagcctaa aacatcacta atgcttgcta tattaagtat
     1381 ttgtttgttt ataatagcgt attataatgc tattacctca accgaccaat atacaacata
     1441 tctgcgctta gttccagtta catcccttac tatagtagca acttacttgt tcttttctca
     1501 atttagtgta ttttttatga agaagctaaa attaaacaag agattttata gaaaaaatac
     1561 aaatatgctt tggatttcga acttgattta taaggtaaag gataatgcta gaatattttt
     1621 cttgataact ataacttctg cagtagcatt tactgctata ggtacagttt attccttttg
     1681 gaaagatgta gagcgtcaaa ttaatttaat atatcctaac acaatatatt attcaacaat
     1741 gacattgcat aatgacccta aaaagcctga tagcaaagaa aaagacaagg aaagaatgat
     1801 gtttatagaa aataatctaa aaaaagagaa tgtagactat acaaggacta atggtgaatt
     1861 taaaactgta tttcccaaga aaagtgattt taatgtcaga attatgaaag aatctaagta
     1921 cttagaaatt acagaaacta taggggttaa gcctataagt tttgaagata atgaatgtat
     1981 atcattatta tcatcaagtt taccaggaaa taagaacatc aaagaaaata ttattgtagg
     2041 gaatagaggt ttaaaggtgg caaaacaagt ggaagaatgt gttatgccag catattacaa
     2101 aaatatgtat gtggtaaagg ataattttta tgatagtata gaatcaaaat ttatagtgga
     2161 tagatttact gcatttgaag tagaggattc aagtaaaact atagagatat gcagaaaatt
     2221 tgaaaataaa tttaataatg agtcagggat gcaaccatat ttgtttttct ctaaagaatt
     2281 aatgatagga actgggaaat taatgtattc tacatttata tttttagcaa tatttatagg
     2341 tttaattttc tttgttacta caagtagttt tttatataat aaattttata tggattgcca
     2401 agttgataag aaaaagtatg aacagttaaa taaattgggt atgacatata atgagattaa
     2461 aaaagcttca acaattgaga ttggaatagt atttttgttt ccttatgtag tggctgttat
     2521 acattcaata tttgcactta ctgcacttaa aaattctttt gatatagaag tagcatcatc
     2581 agctgttttg gtaatgggaa gcttgttctt ggttcaaata gtttattttt taattgtaag
     2641 aaataattat ttaaaagaga taaggttaaa tttagtcaat ttttagttac tattattaaa
     2701 tgataataaa aagggtttag aattaattaa tattactgga attatagatg tttatttttt
     2761 cagtaatatt ttttattgta ttaactatta aaattttgta caaatataaa atataatatg
     2821 gtaaatgtca aaattataaa aaaattctac ttatataaaa tataaagagg ttatatgtac
     2881 atatgtgtag aaataaataa taaaaatatc aattaatgtt attagggggg gttactcatt
     2941 agtaaattag ctaatacact tagaatgatg atattaattc aaagcagagg caaaatgaaa
     3001 tgcaaagaat tatcagaaga gttggaagta agtgaaaggc aaataaaaag ttacaagatg
     3061 tatttagagc aagctggtat atttataaat tcaacacctg ggatttatgg tggttatgaa
     3121 atagataaat gtaatagcat ctcattaatt aaattattag atagtgaagt ttcaattcta
     3181 gatatgataa attcacagtt agagtataat aatgatattt acaaaaatga atttaataat
     3241 attgtagaaa aaataaaagc agtattaaat acaggagaaa aaagcgatac atatatggat
     3301 tattttacag ttcaagcaca atgtaattgt gattatgagt ctgaaaaaaa taagtgtaat
     3361 gaaataatta gagcatatac cacaaaacac aaattttgga tagaatatta ttcattaaat
     3421 tcaggaaata gtgaaagaat tgttcatcca tatggtttat ttaattataa atcagatact
     3481 tatatggtag ctttttgtga aaaaagattt aagtttatag actttaaatt atgtagaatt
     3541 aaagattata aggttttaga agaaaaatat aatgtagata aaagttttag ttgggatgaa
     3601 tatagtaaaa atagtatagg aatttataag ggagaagaaa ttagtgttgt tataaaaatt
     3661 agtcatccat tttcaacaat tataaaggaa aaagtttggg ttaataatca acaaatcata
     3721 gaatatgatg ataaatctat aatgtttaaa gctaaaatga gaggttatga ggagataaaa
     3781 agttggattt taagtatggg agcttatgtt gaggttgtag aaccagatag acttagaaat
     3841 gatatattgt tagagattga aaaaatgaaa aaaatttact gattttttca aataatttat
     3901 acttggacca atatacttca cggtctagtg ttaatatatt aattgagaga ggtgatatta
     3961 tgaaaatgaa aattgaattt agtacaggtt gtataccaat atcttataat tcgctcttta
     4021 tgagtattat aaaagaagca ataagaaaat ctaatgaaga ttactataaa aacatgtact
     4081 attataaaga aaagaataat aaaaaaacta aaaattttac attttcggtt tatataaaaa
     4141 agtatagtat tgaaggtgac aattttatta tagaagataa agtaatatta aatataagta
     4201 ctccagattt agaattgggt tttcatatat acaatgggtt aatgacttct aaaaaatgtt
     4261 tatataaaga ctatgaactt acaagaatta gaatcgattt atcaagagaa aagaaggtaa
     4321 cagaagaaag ggtactattt aatgcattgt ctccaatttg tgtaaagtct aaagaaggaa
     4381 aattcttgga gataactgat gatagataca tagaagaact aaactatata actaatgaag
     4441 tggttaaaaa ttataggggg aatggattaa aaagaaagtt agaatttgaa aatataggtc
     4501 tcaaaaaggt agtagttaaa gagagtttaa gagaatttaa aaaaataaca ggtaaagagt
     4561 atcaatatat taatggttac aaaggtaaat ttatattgaa aggtgatatt gatgatttaa
     4621 atcttatcta caatctagga attggtttta gacgtgctca gggatttgga gatgtagata
     4681 ttttagaatg gaggtgagat aaatgaagtt aagagtttat agaggagatt ggttttttaa
     4741 tatgggtata gttggatttt taaatataat aaagaaagca gataaacaag ctgaaatatt
     4801 tattatggaa gattatatcg aatttgatag cttatttctt gaaaactttc atgagtatta
     4861 ttttaattac tttatggatg aatatgatgt atcaaaaaga attaaaaaaa atattgatta
     4921 tagtataaat tttattaaat ctaagcctga tagaatcaaa gatggaatta agaaaattaa
     4981 agatagtgtg aaacaacaaa atgataagat taaaaaattt gatgaagaaa aatttaacct
     5041 gataaaagag aagctagata gtatgtctaa gataaaaagt tatgatgaat ttgacagttt
     5101 agaagcttta gtagatgaga ctatagatat atttaaaata aaatcaataa atgacaggct
     5161 tacagcaaat ctttataaat atgtagttca agataattat tttggacaag ttagtttttt
     5221 taatgttgct aaggcgaaat tagatttaga tggattaaaa caggtaatgt ttaatgatta
     5281 tttaagacaa attatatatt ttggagagtt agaagattta ttagaagaga atgactatga
     5341 taagttgaaa aactatttaa atgatagatt aaatagtata gctaaggata taaatgaaaa
     5401 aaaagttagt aagtctagta taaatactat agaaaaaatt atgaaggaaa taaataaaaa
     5461 ctttataaag aaaaataaaa gcatagaaga tataaaaaag tatatggata gtttagaagt
     5521 atgtgaaatg tgtggtttat ataagggaat attagatgaa tactcagaaa gtaattttgc
     5581 tccacttgga gtaagcacca ataatgctag gaatatgttt tggaatcagg cgtatgtacc
     5641 atcaatatgt gatatttgta agctaatatt gttctgtact ccagcagggg ctacttatac
     5701 aagaaagaat tatttaatta atgaagaaaa tgagttttat ttatttgtta acatggatac
     5761 ttgtataaat gagctttttg aaagaaataa tagtttaaaa gctcaaaaaa gtgagtattc
     5821 agattccaaa gaggaaaatc cttttaatca gcttataaaa agtatagtag aagaaaatac
     5881 attgaaaagt gaatggcaac ttcgtaatat actttttgtg gaatttaaag caagtataga
     5941 ttctaaaaaa tgtaaaataa attattttaa tattccaact tatttagcaa aattttttac
     6001 agataagtat gcaaataaaa aaatacaaag tatctatgat tacaagctta aatcaaatgt
     6061 actagattta ctcttgaaaa atagggactt gaagcatcta attaataaca ttttaagaaa
     6121 taaagttaaa aatgatatgg aaagcaacaa caaatcaaat atatcaggaa tagattgttt
     6181 tagagtagtc caattaagag cattaataaa tagctacaaa aagggggtat ataaaatgga
     6241 tagtaaaaat ttggagaaga atgatgaaaa gctgagaata atttattatt taggttgtga
     6301 tatacatgac tattttgtta ataaaaattc taaaaataaa ataggtggag taagctataa
     6361 acttttaaat tcagttaaag tcggaaataa aagtgatttt atggatacaa ttataagagt
     6421 ttttatgtca gctgaaaaac agatgccagc atttatttta gatatagaaa ttgaaaaaga
     6481 tttagacttt gaatctatag gtcatgcttt tatatctgga ttaatttctg ggaaatatga
     6541 aggaaaagat aaactaccaa acgaaaagga ggaaaaataa gatgaaaaga gggttaacat
     6601 ttacagtaat agcacaagct caaagtttaa actatggtga agggataggt aatatatcag
     6661 aattaaaaaa attaagtaga gatgatggaa atatatatac atttgcatct cgccagtgtt
     6721 taagatatga catagtacga ttagcagcag aattgtttaa ttggaattta caggttgtcg
     6781 ataaagaaaa aggtactatt caatttaaag ataatataag tataagagaa tcagaagaaa
     6841 tggatttatt tggatatatg aaaacaaata aaaaagatga gaaagataaa aaaggaggtt
     6901 ctttgactag agaagctact gtaagattaa gtaatgcgat ttctcttgag ccatatagaa
     6961 gtgatatgga ttttttaaat aataaaggat ttgcagatag aataggagaa caccctaatc
     7021 ttgctaacat agagcaacat ttaagttact atacatatac agtgactata gatttatcta
     7081 aaataggcaa ggatggagag atagaacttg ataataaaga gaaatgtaga agagtagtag
     7141 aatttttaga aataataaaa gtattgaata gaaatattag aggtagacaa gaaaatttat
     7201 ctcctctatt tgtagtagga ggagtatatg aaatagcaaa tccatttttc ttgggtagaa
     7261 ttaaattaaa aggagataaa aatggattta aaataaataa acaagctata gaagaagtaa
     7321 tccaaggtac ttttttagga aaagatttaa aagaatttac ctatgttgga atggtagatg
     7381 gggtatttat aaataaagaa gaatttaagg gattgtttga agataatttt tttagtgtag
     7441 ataaattttt tggacaacta gttaaagaag taaaggaata ttatggggtg aattaatatg
     7501 aaggttttga aattagattt atttcaagaa actgcatgct acaaaaaacc atttgctatg
     7561 aagattacag agacatatcc actgcctcca tattctacaa taaatggatt aattcacaaa
     7621 atattaaatg ctacagaata tataccattt aatataagtg tacagggaac atatgaaagt
     7681 atatttaata attaccagac aacatatttt tataaaaaag atagtattac atctatgcca
     7741 atgaatagtc atatgctatt gaatgtaaat ttgataattc atataggtgg ggattttgat
     7801 ttattggaag aaatatatga tagtttatta aattatgacg agcacttatc attaggtaga
     7861 aaagaagatt tagtaagaat taatgatatt agatttgtag aagtcaataa atttgaagta
     7921 gatgaggata tacaattaga tgattatgaa aattataagt tatcagaatg taatataaga
     7981 atgcctatat atataccaaa aacaagttta caagatacag atatagatgg tataagttac
     8041 agactcaata attactatga taataatgct gtggaagata aaaaaagggt ttggaataag
     8101 gtagatagtt attatgttga ggatggaaat actatatcaa ttggagatat attattagat
     8161 aatgaaggag atttattata ttttaataat ataaattagg agggagatta tgttatatgc
     8221 taaatctaat ccagtggaaa cacttagaga acatactgat gaacttttaa aacaaatgaa
     8281 tgtattaaga gaaagttatg gaaaaaatat aaatagctta gattttctag aagaggagat
     8341 attttggcaa ttacttgacc ttgttataga atttcatgat attggaaagg cattttcacc
     8401 gtttcaagag ttaataaaaa gcagaatgga tactaaaata aatgaaccta aaatagtaac
     8461 tcatttagaa aataatgtag gccataatta tctttctcct gcatttattg attatagtta
     8521 tatagacagg aaaaaaaata aagaacttag agcagtatta aatcaagtta ttgtatatca
     8581 tcatgaaaga gatatcttta tagataaaga ttttaaaaat ttaatacaaa aaatattaga
     8641 tgaagattta ataaataaag tttatgaact acagcatgaa tttaaagtta gatatccaat
     8701 aaaaacagaa agattgagta aagtatatct acaaagtgta gaaaaaagaa tagataaaaa
     8761 tcacaagtac tataatttat atattatgtt aaaaggaata ctacatagac tagaccatag
     8821 tgcttctgct catgaagttg tggaatgcaa taatgttata aatataggag aacagacaga
     8881 aaattattta atgaaagagt ttggtagttt gagagaggct caatcatttg ctaaatctaa
     8941 tagaaataag aatataatac tgattgcatc tacaggaatg ggaaaaacag aaactgcctt
     9001 aatttggata gataaagaca aggcattttt tacacttcca ttaagagtaa gtataaatgc
     9061 attatttgat agagcaaaaa atatagttgg agttggagaa tcaaatgata cattcttagg
     9121 tttgttacat tcaacagcaa tagattatct agaagaaagt aatcaagaaa attcaagtga
     9181 gattgtagat ttagcaaagt tattatcttg caaactgaca ttttctacaa tagaccaaat
     9241 atttaaattt ccttttttgt atagagggta tgaaaaagtg tactcaacat tagcatattc
     9301 aaaagtagta atagatgaga tacaagcata ttcaccagaa atagcagctg tattggtaaa
     9361 aggtatagaa atgatacata agataggtgg cagatttatg ataatgacag ctactatgcc
     9421 aaccatatat atagatgaat taaaaaaaag aggtgtaatg aatagtaact tagctgaatt
     9481 gacatgtaat acagaaaaaa taagacattg tgtttctatt gtagaaaatt caatagatga
     9541 aaacttaggt aaaataatac aaagtggaat gaataaaaaa gttcttgtaa tagtaaatac
     9601 agttaaaagt gctgtagaaa aatatgagtt aatagaagaa atagttaaac caaaaggaat
     9661 agatgtcaac ttaaatcttt tacattctat gtatatacaa gaagatagag caaaattaga
     9721 aaaatatata aaagaatttg ccaatagtga tagaaatgga atttggatta caacacaatt
     9781 agtagaggca tctcttgata ttgattttga tgagcttcat acagaaaatt caacacttga
     9841 cagtctattt caacgatttg gtagatgtta tagaagtcgt gagtatgaag aaaatagccc
     9901 aaatatattt atatatacag aaaaagctac aggaataggt agtatttatg ataaggatat
     9961 tgtagaaaaa ggacttgagc tactgaaaac ttttataaat ggaaaagaat gtgtgaagat
    10021 gaaagaaaaa tataaggtag aaatggttaa gatactatac tctaaagaaa gtctagaagg
    10081 tacagctttt gaaaagagat ttaccagtgc tttaaatatt ttagatacta taacgccata
    10141 tagtttaggt tctaaagatg cacaaaatat acttagaaat attgaaggat atacagtaat
    10201 tccagaggat atatataata aaattgaaga aactttaatc aaagattatg aagagttggg
    10261 acaagagctt ataaatgcat atagtaatga tgatagacaa ttgataaata aaataaagtc
    10321 taagagaaaa aaagcaagaa gagagataat taaaaaaact gtaaatttac caatatataa
    10381 agctaaacaa aatgtaattg acatacaggt gaaaggtctt gaagatttaa aaatactttt
    10441 atataaatat gatatatacg aaaataagca tacaaaacag atatttggta aaggtgtgct
    10501 tataagtaat gaaatagacc aatttatttg atttttatat taacaatgtg gctaaaattt
    10561 actaaagaag gtgattttgt gccaatagat ttagaagtat taaaagttca aggtgtaaaa
    10621 ataaattatt attatgtatg taaaagaaaa ctatggctat tttctaaagg aataaccatg
    10681 gaacaaaata gtagtagagt attgtctggg aagatagtac atgaagattc atataaaaga
    10741 atgaaaaata aagaaatatt gattgatgat atcttgaagc tagatataat aaaggatgag
    10801 tatgtaaaag aagtaaaaat aagttcaaag atgaaagaag cagatgaaat gcagttatac
    10861 tattacttat actatttaaa gaatagtatg ggtatagaga aaaaaggaac tataaattat
    10921 gttaaggaaa agaaacaaga agaattgata ttgacagaag aaaaagaaag aattattgaa
    10981 aacacactta taaatataaa tgaaataaat atgcagttat ctccttccaa aattgagaaa
    11041 aaacattatt gtacaaaatg tgcttattat gaattttgtt ttgtgaagga ggagtaacaa
    11101 tgtataaaga ctattatatt ataagtaatg gggatataaa aaggaaagaa aatacgattt
    11161 actattacaa tttagaaggt gagaaaaaaa tattaccagt agaacaagtt gatgatattc
    11221 atatatatgg agaagtagat ttaaacacaa aacttataaa ttacataagt aaatatgggg
    11281 tgatgcttca tttctacaat tactatggat attattcagg tacatattat ccaaggaaga
    11341 aaaatgtatc aggattcagt ttagtatgtc aatcagcctg ctatttagat aaaaatgaaa
    11401 gattatactt agcaaaatcc tttgtggaaa gtgcatcata ccatatttta agaaatatga
    11461 gatattacaa agttgatgaa agtttaattg atactataaa ggatgaacta aatgatttga
    11521 atgatgtcaa agaaatatca gaacttatgg gtaaagaagg cagaataaga aaaacatact
    11581 ataaatcatt taatcaaata ttaaaagatg attttgaatt ttataaaaga gaaaaaagac
    11641 ctcccaaaga cccagtaaat gctcttatgt cttttggaaa tagcataatg tatacgaatg
    11701 ttttaagtga gatatataag acacaacttg accctacaat aagcttttta catgaaccat
    11761 caacaaagag attttcttta agtttagata ttgcagaaat ttttaagcca attatagttg
    11821 acacaataat ttttagcctg attaataata aaagaattaa taagaatgat tttgaatatc
    11881 aagacaaaat ttgttattta agtgaaaaag gaaagaagaa atttttgcaa gaatttgaaa
    11941 ataagatgca gacaactata aaacatagaa atctaaatag aaaagtttca tatagaatgt
    12001 ttataaggtt agagtgttat aaattaatta aacattttat aaatgatgaa aggtacaaag
    12061 ttttaaaggt gtggtggtaa gtatgtttgt tattgttact tatgatattg ttgaagcaag
    12121 gtcgttaaat agaattagaa ggatacttag aaaatatttg acttggacac aaaattctgt
    12181 ttttgaaggc aatattactg aaggaaagtt acataaatgt atttctgaaa tagaaaatat
    12241 tattgataat agcgaggatt caatctatgt ttatgagata aaaaatccta attcaattaa
    12301 aaagaaatgt tatgggattg ataagtattc tgatgaaatg tttatatagg tttgcagtga
    12361 gcgatattta tgctaaaata ggtgttaaca gttggaatat aagggattga aggtgtatga
    12421 taactgttat caattgcact actgctcgct cactgcaaat tttgatgttt ttattgaatt
    12481 ataattgctt gattgaagtg ttttcaatgt attcaaatat acctattttg gggttttata
    12541 ttaactaagt ggtatgtaaa ttggaaaact tgtcaaaaat agcatcaaag catttatagt
    12601 tttatattaa ctaagtggta tgtaaatggg gcttttgggg acatgagtgt tacagatgca
    12661 ctaagtttta tattaactaa gtggtatgta aatagccccc taatgctgga acagtgagga
    12721 cacacaaacg ttttatatta actaagtggt atgtaaatga acttttaaaa gattatgcta
    12781 ggaaaattca tgttgctggt tttatattaa ctaagtggta tgtaaataag ccaaaagcta
    12841 cgaatttgaa ctaaaatgcg tgggttttat attaactaag tggtatgtaa attgcctagg
    12901 gaacccctcc agcccctaac catgcaacag ttttatatta actaagtggt atgtaaataa
    12961 caagaaaaac caacaacaaa accaacaaca aaaaggtttt atattaacta agtggtatgt
    13021 aaatctttcc ataaaatacc ccctacttca tttttattta gttttatatt aactaagtgg
    13081 tatgtaaata agaccaagcc acttccgcaa ttgcatcttc taacatgttt tatattaact
    13141 aagtggtatg taaataaata ttttgttgac ttatttcaaa ctttagagga cgttttatat
    13201 taactaagtg gtatgtaaat agtgtatttt tatcattcta tctactatac caaaaaagtt
    13261 ttatattaac taagtggtat gtaaatgcat acctgtaacc tgcatcattt tgtatctatc
    13321 ttgttttata ttaactaagt ggtatgtaaa taaaagattg taatgagtac gagacaagat
    13381 ttattgtgtt ttatattaac taagtggtat gtaaattata taaattttct ttttcttcta
    13441 tttctaagtt cagttttata ttaactaagt ggtatgtaaa tgttgtatag ctagtacttc
    13501 ctgctttaaa tccttttcgt tttatattaa ctaagtggta tgtaaataag acaaggttct
    13561 tcttttaagt ggcaagctat aaaagtttta tattaactaa gtggtatgta aatcgagtaa
    13621 aataacaatt atctggaatg tgagaagttg ttttatatta actaagtggt atgtaaatta
    13681 cacataatta caacccagtt tactttttgc tacacgtttt atattaacta agtggtatgt
    13741 aaattatgaa tatattacat aatcataaag atttacaaat ggttttatat taactaagtg
    13801 gtatgtaaat tctctaaatc catctataac gttaccagca aaatcaattt tatattaact
    13861 aagtggtatg taaataagaa cttttcatga acgtggaaaa tattgagttc agttttatat
    13921 taactaagtg gtatgtaaat ttgtcattta cgactaccat ttctctagca tttatatgtt
    13981 ttatattaac taagtggtat gtaaatggga aaatatggga aaagttaact aatataatgc
    14041 aaagttttat attaactaag tggtatgtaa atgaaatttt tagtgctatt gcaactactc
    14101 ttgtttgtgt tttatattaa ctaagtggta tgtaaatgca taaccaaaaa cttcaaaatc
    14161 aagacctcga atttgtttta tattaactaa gtggtatgta aattgcgcaa taacatgctg
    14221 aaaactgcaa tttcgaagtg ttttatatta actaagtggt atgtaaatag gataaagaaa
    14281 agactcacac aaggcacagt gtcaggtttt atatcaacta agggtagata agtagaatat
    14341 tagcaatatc aacgagtatt tagaaactta ttttatatta ataaagcagt atataaaatt
    14401 gtagaatcaa caatagcata tactaaaaca tccaccttat attaactaaa tcatatctaa
    14461 ataaaataca cttacctata aacattataa aatcaataca aaaatgaggt gaaataaaat
    14521 ttatgataaa gaaattaaac aataaagaca taaataaaat catggaaata tgggaaaaaa
    14581 gtacaatcaa agcacatgac tttataagta aagaatactg gcaaaataac tataatactg
    14641 ttaaaaacga atatatacct atatcagata catttgtata tgatgatgga gatgaaataa
    14701 aaggatttat aagcataata gataaaagct ttataggagc tttatttata aagcccaaat
    14761 accaaaatct aggtatcgga ggtaaacttt tagattatgc aactaaaaaa tataaaagtc
    14821 taagcttagc agtatataaa gataataaaa aagcagttgt gttttataat aaaaaaggtt
    14881 ttaatatagt aaaagaacaa gtaaatgaag attcaggatt taaagagtac ataatggaat
    14941 atagtaaata atatgattac attctattaa aaggataatt aatttatttg ccatttgatt
    15001 ttttaagagt aatttttaaa atagactata tgaag
//
LOCUS       DAEOSP010000092        14739 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.092, whole genome shotgun sequence.
ACCESSION   DAEOSP010000092 DAEOSP010000000
VERSION     DAEOSP010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14739
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.092"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..1174
                     /locus_tag="KRH54_002145"
     CDS             <1..1174
                     /locus_tag="KRH54_002145"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015983.1,HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG8579802.1"
                     /translation="YFLLGQENKIKIYDFVNYFDDESDKKYIINLIDIMNKNQIFKSN
                     NLNFDMLSNGVAIYFCLTNRCNLFCKHCCVESSSNKKDLLSTYDLKNIVDIISVLNTP
                     HIIITGGEPLIREDFFEIIKYIKQKLPDTKLILSTNGTLIDEHNVDFIITNFEKIDIS
                     IDGVDENTCSETRGKGVFNKVISSVNLLKDNGFSNIKLSMVFGQKNSHLNKQFKDLNK
                     KLGTEFVERYFIPTGRGLENKESYLIEDTVLPITIPYILDNELKMCKDKKNKKMGCCA
                     CNAGNSQIFINYDGDVYPCPSLIKDDYKMGNIFDNEFVKVIKENKTRDLKSLIKLESI
                     YPYNFEKCKDCDVNIFCWHCPAILDSVKDNELELDRWCSMMKHNLNTAVWGDEKVN"
     gene            1171..2187
                     /locus_tag="KRH54_002146"
     CDS             1171..2187
                     /locus_tag="KRH54_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG8579803.1"
                     /translation="MKFTIFVTEDCNLRCTYCYEGNDKKKENMSYETADKTIDFICNK
                     ELESNSDYTTIVLHGGEPFINFDLIKYIHRDLVDRLSDKKILFDITTNGTIINDEIIE
                     FIAKNVKNISISIDGKKNIHDINRIDANGVGTYEKVMENGKRLIDKGIYVRARMTYNS
                     NTVKYLYESVYDIAKQGFKNIAPISDFMDKNWDNSHIKILNEQISKIMNIKKDYPEID
                     ISILDKQFINKKKGDCFGGINSYAIDTRGDVYPCTVCVGDKDILLGNIYDGLDDKKIK
                     NLIKIYISKNEECDGCTRLDYCMSTRCKLINKKVTGKFQSAVPIYCGLERVYVETTKK
                     LCSL"
     gene            2243..2404
                     /locus_tag="KRH54_002147"
     CDS             2243..2404
                     /locus_tag="KRH54_002147"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579804.1"
                     /translation="MIHIKKIEEKIELQDVEVQADPCKWYYDKHGIISQIDCMVDCAG
                     STPKGTVAY"
     gene            2612..5158
                     /locus_tag="KRH54_002148"
     CDS             2612..5158
                     /locus_tag="KRH54_002148"
                     /inference="COORDINATES: protein motif:HMM:NF014717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8579805.1"
                     /translation="MINIKLAVSYLKRQKGKTLSLILSIGIAVMLVFSLSVINESQGQ
                     SDINHAYKNSGDYHAFYEDINSDIVKELNGEEDIKEFNDVLKFGEIISKDNGASLNLC
                     SYNKDFLDSTRYKLVKGREPRTSNELVIEQKVLDKMELDVKLNQTIDFQIVNEHINKN
                     NENSIYSKDKQFKIVGILSKSDEYYENLNKYKVRAFTYSNNGKDIVPYELITHMGTLK
                     LNTKDLDSQKVVNLGRKYKLTSNQFYPNTEVSMSMYMKNISKKTTYSIKQSLLPLLCA
                     TLVIYNMFNIIILDMTKQIGLLRAVGASKRNIRQVFFIQSIVVLSVGIVIGLLGGIVF
                     SYLGLMIVYDKSAELTINTNSIIESIIMSIIAVILASVVPIYKAGNLSIIDAIKKTDK
                     LNYIPRLLLLKSHKKVVGIINEIAFKNIFRNKVTSIIIIITISLCGVLFIGRLTSTKF
                     IYGKESDSANITSKSYGNFDIYLGYSPINANYTFSKYDNSLLNEIKAIEDVTDVEPNI
                     YLKGYLRNADLEEDYLDELKRTEINNNNESTVLIRGYNKELLNNIDKYIDKGENVYSY
                     TDRDYKNVLLVNNFYSRIKLSNNTQIIKSPKIGDLIDIKIPVYKDGEYKYINTKVRIA
                     GIMKNSYISEQDGESQSGGIQIIFNEKDLKELTGISDYNKVFVKIKKETDKKVIKEIN
                     NIIEKSGFSDIEGRYVRNHFGDHYNKSSYKLAMICVFGVLLISSINIIFIVRSNIIVR
                     LSEICTFRAIGMSKKNVKRMLIKENMIYGVLSMIVAGIISSFNYYKIVSETNKLNQQV
                     YGINNSIKFEIPIYEILIFGSISVFVCVIAVYFSNKMINKLSIVSGIKEN"
     gene            5200..6444
                     /locus_tag="KRH54_002149"
     CDS             5200..6444
                     /locus_tag="KRH54_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG8579806.1"
                     /translation="MNSSNKYFLDCICEKIINNNQVILSNKVNGRWVKIPIECYEVLK
                     YSVDNALQLGNLYDVFQENEDKKYYERVIDKLEHIGLLSDSILSEFRLERINKISFSP
                     TNRCNLSCDYCCVDSKIDNNDYLDTSQVRKVIDNIVKLNPRILTITGGEPLMRNDFLE
                     LLDYIKEKFKGKIILSTNATLIKEKQIDDIINGLYAIEISLDGYDELSCSEVRGKGVF
                     CKVLNVIRLIKLKKFENVGLSMVVGKNNEKDIEKFNKLNEDLGTTPIIRNFMNIGRGN
                     ENYSKYLNDKLDIDYICKDDYVDESSLCSNMCKAGVNQISIDHKGDVYPCPNLEYKDL
                     KIFNILEFNENILERILNREMDIFNIFDKLKPVNIESCKECKVNLFCTTCPSKIYTLK
                     NNKKMFDANCKKMKNILEPILW"
     gene            6492..7481
                     /locus_tag="KRH54_002150"
     CDS             6492..7481
                     /locus_tag="KRH54_002150"
                     /inference="COORDINATES: protein motif:HMM:NF012311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBG8579807.1"
                     /translation="MTKVRIAVLDTGLDVNDDDINGNYVYDSNFQLKDLGGKEINDIN
                     GHGTFVSKTILNISKNTQIYPINIFNDSGKTSSLNLLSTLSKLTMSDIQIINISAASI
                     NSMYEKELEKVCNILNSQGRFIICSKHNKYEDKESIPTIFESVIGVVGNENIYKNNQY
                     IYEPENKIQMYANSKDNFIKFKDKVTHFGKNSKACAVATGIVANILKNNPYIKFNELE
                     EILIQKSKSKEDLKIENLNTPSYKSNLIRLDIARKIINVINRNFSKIKIDFEFVSEYS
                     LFNNFTCIGNYNAYNFLTILNKEFNISIDYKEVYLYQLENLDMAVDLIYENLI"
     gene            7518..8192
                     /locus_tag="KRH54_002151"
     CDS             7518..8192
                     /locus_tag="KRH54_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8579808.1"
                     /translation="MKILKVKDLKKIYGKYENEVRALDGIDLDIEPHKFTAIVGASGS
                     GKSTLLHCVAGLDKPTLGNVYLDDVDLYTLNDDKLSEIRRQEFGFIFQSYNLIPVINV
                     YDNIVLPVLLDGKKEDKEYVMRLINKLGIEKQIKKYPNELSGGQQQRVAIARALANRP
                     SVIFADEPTGNLDSKTTNEVMNMLEWCVKEYKQTLVMITHNDNIAKTADRIITIKDGK
                     IDEKNY"
     gene            8176..9918
                     /locus_tag="KRH54_002152"
     CDS             8176..9918
                     /locus_tag="KRH54_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8579809.1"
                     /translation="MKKTINNNLFRILNIIVYKNKTKLIVSFIMVFIIAGVNIVIPQL
                     IRCIIDDGIGNKNIFLLINLILIYIFIRILSATFDVILSYLYTSIKNKVTINLKVKLL
                     NHISKLSGRYYSSKKTGNILSIMESDIFMIENFGAEIIFDIIVNVFTAIISLIFLINL
                     QFDLFLVVLFLQLILTFSQNKMTKLISKKISEIRSDSGSLSNLIQEYVSNIMDIVILN
                     SKKNFFNKYLKRERDLIKKDLNLNIIMSSNIAISQILTGLITILIYGIGGYKIINNKM
                     TMGELIAFQQYTGMLIGPCIAIVRANTRIAQSKVSINRVFSVIDEKIDIKVDNNGYRI
                     DNSEIKKIDFKNVSFRYGEDLNLVLNDINLQFEKGEVTGIVGGSGCGKSTITNLIYRL
                     WDINEGDILIDGVNIKDINLKSLRNNIDIVTQDQFVFDDTIKNNIVLNNKISDKELDE
                     ILEVTCLDDFIKNLDKGLDEVVGEKGVKISGGQKQRISIARALVSNKKVLILDEATSA
                     LDNISQKEILNNLKEYIQDKIVIIIAHRLSTIKSADNIYVLDKGRVIESGNDAELLHK
                     DGLYNKLVETADFA"
     gene            complement(10333..11577)
                     /locus_tag="KRH54_002153"
     CDS             complement(10333..11577)
                     /locus_tag="KRH54_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG8579810.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
     gene            complement(11620..14454)
                     /locus_tag="KRH54_002154"
     CDS             complement(11620..14454)
                     /locus_tag="KRH54_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8579811.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSIVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
BASE COUNT         5802 a         1535 c         2239 g         5163 t
ORIGIN      
        1 gtattttctt ttaggacaag aaaataaaat aaaaatttat gattttgtaa attattttga
       61 tgatgaaagt gacaaaaaat atattattaa tcttatagat attatgaaca aaaatcaaat
      121 ttttaaaagt aacaatttaa actttgatat gttaagcaat ggtgttgcaa tttatttttg
      181 tttgacaaat agatgcaatt tattttgtaa acattgttgt gttgaatctt cttctaataa
      241 gaaagattta cttagtacat atgatttaaa aaatatcgta gatataatat cagttttaaa
      301 tactcctcat ataattataa ctggtggaga accattgata agagaagatt tttttgaaat
      361 aattaaatat ataaaacaaa aattacctga taccaaatta atattatcca caaatggaac
      421 tttaatagat gaacataatg ttgattttat tataactaat tttgaaaaaa tagatattag
      481 tattgatgga gtagatgaaa atacttgtag tgaaacacga ggaaaaggtg tatttaataa
      541 agtaataagc tctgttaatc tgctaaaaga caatggattc agtaatataa agctgtctat
      601 ggtatttgga caaaaaaatt ctcatttaaa taaacaattt aaagatttaa ataaaaagct
      661 tggaacagaa tttgttgaaa gatattttat tccaacagga agaggcctag aaaataaaga
      721 aagctattta attgaagata cagttcttcc aattacaatt ccgtatattt tagataatga
      781 attaaaaatg tgtaaagata aaaaaaataa aaaaatggga tgttgtgcat gtaatgcagg
      841 aaactctcaa atatttataa attatgatgg agatgtatat ccttgtcctt cactgataaa
      901 ggatgactat aaaatgggaa atatatttga caatgaattt gtaaaagtta taaaagaaaa
      961 taaaacaaga gatctaaagt ctttgattaa attagaaagt atttatccat ataactttga
     1021 aaaatgtaaa gattgtgatg taaatatttt ttgttggcat tgcccagcga tattagattc
     1081 agttaaggat aatgaattgg aattggatag atggtgtagt atgatgaaac acaatttaaa
     1141 tacagctgta tggggagatg aaaaagttaa ttgaaattta ctatatttgt aactgaagac
     1201 tgcaatttga gatgtacata ttgttatgaa ggtaatgata aaaaaaaaga aaatatgagt
     1261 tatgaaactg ctgataaaac aatagatttt atttgtaata aagaattaga aagtaatagt
     1321 gattatacaa caattgtttt acatggagga gaaccattta taaattttga tttgattaaa
     1381 tatatacata gggatttagt agatagattg agtgacaaaa agatattatt tgatataact
     1441 acaaatggaa ctattataaa tgatgaaata atagagttta tagcaaagaa tgtaaaaaac
     1501 atatcgataa gtattgatgg aaaaaagaat atacatgata taaatagaat agatgctaac
     1561 ggagtaggta cttatgaaaa agtaatggaa aatggaaaaa gacttataga taaaggtata
     1621 tatgttaggg caagaatgac atataactca aatactgtta agtatcttta tgagagtgta
     1681 tatgatatag caaaacaagg gtttaaaaat atcgcaccaa tttcagattt tatggataaa
     1741 aattgggata attctcatat taaaatatta aatgaacaaa ttagtaaaat tatgaatatt
     1801 aaaaaggatt atcctgagat agatatatct attttagata aacaatttat aaataagaaa
     1861 aaaggagatt gttttggagg gataaatagt tatgcaatag atactagagg agatgtatat
     1921 ccatgtacag tatgtgtagg agataaagat attttactag gaaatatata tgatggatta
     1981 gatgataaaa aaattaagaa ccttataaaa atttatatat ctaagaatga agagtgtgat
     2041 ggatgcacta gacttgatta ttgtatgtca accagatgta aacttatcaa taaaaaggta
     2101 acaggtaaat tccaaagtgc agtaccaatt tattgtggat tagaaagagt ttatgtggaa
     2161 acgacaaaaa aactatgtag tttataatct agtttaacta gttataataa atataaaaca
     2221 aaagtaaagg gggatatgta ttatgataca tataaaaaaa atcgaagaaa aaatagaact
     2281 tcaagatgtt gaggtgcaag cagatccatg taaatggtat tatgataagc atggaataat
     2341 ttcacaaata gactgtatgg tggattgtgc aggatcaact ccaaaaggaa cagtagctta
     2401 ttaaaaaata ctaatcagtc tattaaatat gttgaaagta ctagatgtag ttttttacaa
     2461 atgaaatgtg attttctcaa ctctagtatt tttaataatt aaatttaact agcacaacga
     2521 gttagttagt atttaatgaa tagtgctcta taacctacta ttttaggtta tagagttttt
     2581 cttgtaaata tgaataagga ggattctgat tatgataaat attaaacttg cggtgtcata
     2641 tttaaagaga caaaaaggaa aaactttatc attaatactt agtataggga ttgctgttat
     2701 gctagttttt tcattaagtg ttattaatga atctcaagga caatcagata taaatcatgc
     2761 atacaaaaac tcgggagatt atcatgcttt ttatgaagat ataaatagtg atatagtaaa
     2821 agaacttaat ggtgaagaag atataaaaga attcaatgat gtacttaaat ttggagaaat
     2881 tataagtaaa gataatggag catctctaaa tttatgctct tacaataaag attttttaga
     2941 ttctactaga tataagttag ttaaaggtag agagcctaga acttctaatg aattagtaat
     3001 agaacaaaaa gttttagaca aaatggaatt agatgtcaaa ttaaatcaaa ctattgattt
     3061 tcagattgtt aatgagcata taaataaaaa taatgaaaat tctatttata gtaaagataa
     3121 gcaatttaaa atagttggta ttttaagtaa atctgatgaa tactatgaaa atttaaataa
     3181 atataaggta agagctttta catattcaaa caatggcaaa gatattgttc catatgaatt
     3241 aataactcat atgggtacat taaaattgaa tacaaaagat ttagattctc aaaaagtagt
     3301 gaatctaggg agaaaatata agttaacaag taatcaattt tatccaaata cagaagtaag
     3361 tatgtctatg tatatgaaaa atatttcaaa aaaaacaact tattcaataa agcaaagctt
     3421 actcccttta ttatgtgcaa cattagtaat atacaatatg tttaacataa taatactaga
     3481 tatgaccaag caaattggtt tacttagagc tgttggagct agcaagagaa atattagaca
     3541 ggtatttttt attcagagta ttgtagtttt atctgtaggt atagtaattg gtttacttgg
     3601 aggtatagtt ttttcttact taggtctcat gatagtatat gataaaagtg cagaattaac
     3661 tataaataca aactctataa ttgaatctat catcatgtca attatagcag ttatattagc
     3721 ctctgtagta ccaatatata aagctggaaa tttatctata attgatgcta taaaaaagac
     3781 agataagtta aattatattc ctaggctttt gttgttaaaa tcacataaaa aagttgttgg
     3841 aattataaat gagatagctt ttaaaaatat atttaggaat aaggttacat caataataat
     3901 aataattacg atatcactat gtggggtttt atttataggc agactaactt ctacaaaatt
     3961 catatatgga aaagagtcag attcagctaa tataacaagt aaatcatatg gcaattttga
     4021 tatttatttg ggatattcac caataaatgc aaattataca ttttctaaat atgataattc
     4081 tttattaaat gaaataaaag ctattgaaga tgtaacagat gttgaaccca atatttattt
     4141 aaaaggatat ctaagaaatg ctgacttaga agaagattat ttagatgagc taaaaagaac
     4201 tgaaataaat aataataatg agtctacagt tttaattcga ggttacaata aagagctatt
     4261 aaataatata gataaatata tagataaagg cgaaaatgtt tatagttata cagacaggga
     4321 ttataaaaat gttttattgg taaataattt ttattctaga attaaattgt caaataatac
     4381 tcagattata aaatcaccta aaataggaga cttaatagat ataaaaattc ctgtatataa
     4441 agatggagag tataaatata taaatacaaa agtaagaatc gctggaataa tgaaaaatag
     4501 ctatatatca gagcaagatg gagaatctca atcaggtgga attcaaatta tatttaatga
     4561 aaaggattta aaagaattaa cagggataag tgattataat aaagtttttg tgaagataaa
     4621 aaaagaaaca gataaaaaag taataaaaga aataaataat ataatagaaa aaagtggatt
     4681 ttcagatata gagggaagat atgtacgtaa tcattttgga gaccattata ataaatcctc
     4741 atataaatta gcaatgattt gtgtatttgg agttttatta atatcatcta taaacattat
     4801 ttttatagtt agatcaaata taattgtgag gttgagtgag atatgtacat ttagagcaat
     4861 agggatgtcc aaaaaaaatg taaaaagaat gttaataaaa gaaaatatga tatatggggt
     4921 attaagtatg attgtagctg gaattatatc tagtttcaat tattataaaa tagtttctga
     4981 aactaacaaa cttaatcaac aggtatatgg tataaataac tctattaaat ttgaaattcc
     5041 aatatatgaa attctaatat ttggatctat atctgttttt gtttgtgtca ttgcagttta
     5101 tttttctaat aaaatgataa ataaacttag tatagtaagt ggtataaaag aaaattaatg
     5161 aaatttctaa tttaaatata taggaaggaa ataaaataaa tgaatagctc aaacaaatat
     5221 tttttagatt gtatatgtga aaaaataata aataataatc aagttattct tagtaataaa
     5281 gtaaatggaa gatgggttaa aattccaata gaatgttatg aagttctaaa gtattcagta
     5341 gataatgcat tacaacttgg taatttgtat gatgtattcc aagaaaatga agataaaaaa
     5401 tattatgaac gagttataga taagttagag catataggac ttctaagtga ttctattcta
     5461 agtgaattta gactagagag aataaacaaa atttcatttt ctccaactaa tagatgtaat
     5521 ctaagttgtg attattgctg tgtagattct aaaatagata ataacgatta tttagataca
     5581 tctcaagtaa gaaaagtaat agataatata gttaaattaa atccaaggat attaaccatt
     5641 acaggtggag agccattgat gagaaatgat ttcttagagc tgttggatta tataaaagaa
     5701 aaatttaaag gtaaaataat tctgtctaca aatgcaactt taattaaaga aaaacagatt
     5761 gatgatataa ttaatggttt atatgcaata gaaattagtt tagatggtta tgatgaatta
     5821 agttgtagtg aagtaagagg taaaggagta ttctgtaaag tattaaatgt gattagactt
     5881 ataaaactaa aaaaattcga gaacgtaggt ttgtctatgg tggtaggaaa gaataatgag
     5941 aaagacatag agaaattcaa taaattaaat gaagatttag gtacaacgcc tataataaga
     6001 aattttatga atattggtag aggtaatgag aactatagca aatatcttaa tgataaactt
     6061 gatattgatt acatttgcaa agatgattat gtggatgaaa gtagtttatg ttcaaatatg
     6121 tgtaaagcag gtgttaatca gataagtata gatcataaag gagatgtata tccgtgtcca
     6181 aacttagagt ataaggattt aaagatattc aatatattag aatttaatga aaacatatta
     6241 gagaggattc ttaatagaga aatggatata tttaatatat ttgataaatt aaaaccagta
     6301 aatattgaga gttgtaaaga atgtaaagta aatttatttt gtacaacttg tccatcgaaa
     6361 atatatactt taaaaaataa caaaaaaatg tttgatgcaa attgtaaaaa aatgaaaaac
     6421 atcttggaac ctatattatg gtaaggatta gttttatatt caaggtaatt tattttagag
     6481 gtgattatta attgactaaa gtaagaatag cagtcttaga cacaggatta gatgtaaatg
     6541 atgatgatat taatggaaat tatgtttatg attcaaattt tcaattgaaa gatttggggg
     6601 ggaaggaaat aaatgacata aatggacatg gaacttttgt atcgaaaacc atattaaata
     6661 taagtaaaaa tacccaaatt tatcctatta atatatttaa tgattctgga aaaacaagtt
     6721 ctttaaatct tcttagtaca ctgagcaagt tgactatgtc ggatatacag ataataaaca
     6781 tttcagctgc atctattaat agtatgtatg aaaaagaatt agaaaaggtt tgtaatattt
     6841 taaatagtca aggtagattt attatttgtt caaaacataa taaatatgaa gataaagaaa
     6901 gtattcctac tatatttgaa agtgttattg gtgtagtagg taatgaaaat atatataaaa
     6961 ataatcaata tatatacgaa ccagaaaata agattcaaat gtatgcaaat tcaaaagata
     7021 attttattaa atttaaagat aaagtaactc attttggtaa aaatagtaag gcttgtgcag
     7081 tggcaactgg cattgttgca aatatattaa aaaacaatcc atatattaag tttaatgaat
     7141 tggaagaaat acttattcaa aaaagcaaaa gtaaagaaga tttaaaaata gaaaacttga
     7201 acactccaag ttataaatct aatttgataa gattggatat agcaaggaaa ataataaatg
     7261 tgataaatag aaacttttct aaaataaaaa tagactttga atttgtaagt gaatattctt
     7321 tatttaacaa ttttacatgt attggaaatt ataatgcata taatttttta actattttaa
     7381 ataaagagtt taatattagt attgactaca aagaagtata cttatatcaa ttagagaatt
     7441 tagatatggc agtagatctt atttacgaaa atttaattta aaaatatatg ttttttaaat
     7501 gggaatggag atacgttatg aaaatactta aggtaaaaga tttaaaaaaa atatatggaa
     7561 aatatgagaa tgaagttaga gctcttgatg gtattgattt agatatagag ccacataaat
     7621 ttacagctat tgtaggtgca agtggttctg gtaaaagtac actacttcac tgtgttgcag
     7681 gcttagacaa gccaacttta gggaatgtgt acttagatga tgtagattta tatacattaa
     7741 atgatgataa gctgtctgaa ataagaagac aggaatttgg cttcatattt caaagttaca
     7801 atttaatacc agttataaat gtatatgata atatagtact tcctgtgttg cttgatggga
     7861 aaaaagaaga taaagaatat gttatgagat taattaataa gttggggatt gaaaaacaaa
     7921 taaagaaata tccgaatgaa ctatcaggag gtcaacaaca aagagttgca atagctagag
     7981 ctttagccaa tagaccgtca gtaatatttg cagatgaacc tactggcaac ttagatagta
     8041 agactacaaa tgaagttatg aatatgcttg aatggtgtgt taaagagtac aaacaaacac
     8101 tagttatgat aactcataat gataatattg caaagacagc tgatagaatt ataactataa
     8161 aagatggaaa aatagatgaa aaaaactatt aataataatt tatttagaat actaaatata
     8221 atagtatata agaataagac taagttgata gtatcattta taatggtttt tataattgct
     8281 ggcgtaaata ttgtaatacc tcaactaatt agatgtataa tagatgatgg aataggaaat
     8341 aaaaatattt ttctgcttat aaatttaata ttgatatata tttttataag gatattatca
     8401 gctacatttg atgttatatt atcatattta tatacttcaa taaaaaataa agttactata
     8461 aatctaaaag taaagttgct taaccatata tcaaaacttt ctggtagata ttattcaagt
     8521 aagaaaacag gtaatatttt atccattatg gaaagtgata tatttatgat agaaaacttt
     8581 ggagcagaga ttatatttga tattattgta aatgtgttta cagctataat atcgttaatt
     8641 ttcttaatta atttgcagtt tgatttattt ttagtagttt tatttttaca gttgatatta
     8701 acattttctc aaaataaaat gacaaaactt atatctaaga aaatttctga aataagaagt
     8761 gattcaggca gtttatcaaa tttgattcaa gagtacgtat ctaatattat ggatattgtt
     8821 atattaaatt caaaaaagaa tttctttaat aagtatttaa agagagagag agatttgata
     8881 aaaaaagatt tgaatttgaa catcattatg agtagtaata tagcaatatc tcaaatacta
     8941 acaggattga taacaatatt aatatatgga attgggggat ataaaataat aaataataag
     9001 atgacgatgg gggagttaat agcatttcaa caatatacag gaatgttaat tggaccatgt
     9061 atagctatag taagggcgaa tactagaata gcacaatcaa aagtgtctat caatagagta
     9121 ttttcagtaa tagatgaaaa gatagatatt aaagttgata ataatggata tagaatagat
     9181 aacagtgaaa ttaagaaaat agattttaaa aatgtatctt ttagatatgg tgaggattta
     9241 aaccttgttt taaatgatat aaatttacaa tttgagaaag gggaagtaac aggaattgtt
     9301 ggtggaagtg gatgtggcaa atcaacaata acaaatctaa tataccgact ttgggatata
     9361 aatgaaggtg atatattaat agatggtgtt aatataaagg atattaattt aaaatcacta
     9421 agaaataata ttgatatagt aactcaagat caatttgtat ttgatgacac aataaaaaat
     9481 aatatagtat taaataataa aataagtgat aaagaattag atgagatatt agaagttact
     9541 tgtttagatg attttataaa aaatctagat aagggattgg acgaagtagt aggagaaaaa
     9601 ggtgtcaaaa tatcaggagg tcaaaaacaa cgtatatcaa tagctagagc tttagtaagt
     9661 aataaaaagg tacttattct ggacgaagca acttcagctc tagataatat ttcacaaaaa
     9721 gagatattga acaacttgaa agagtacata caagataaga tagttattat aatagctcat
     9781 agactttcaa caattaagag tgctgataat atatatgtat tagataaagg tagagtaata
     9841 gaatctggaa atgatgctga acttctacat aaagatggtt tatataataa gttagttgaa
     9901 actgcagatt ttgcataagc atgactttgt atgtacatat atgattatta gggtatttaa
     9961 tggaatctat aagttatata actaatttag ttatataaaa gtttgcaaat caatctcaga
    10021 taatgttaaa caaatgatta gtatatccag tagaaaaatt ataatagtag tagaatatat
    10081 tacaaataaa tcaaaacttc acataatttt catatgaatt atcaattaaa ttcacacaca
    10141 agagttatta tataaacata gaagatagta aaactagctt aaaaacataa tataataccc
    10201 tatacaaaat agtggcaact ctccctcaca tcctcaattg ttgccactat tttaattttt
    10261 tgtaaaaaac aaaaagactc cacaaagtgg agtctttata aatttaacat acccttttaa
    10321 taatatgctt tactatgctt taaaacaaac agaattttta atctcaagtt ttttatttat
    10381 cttagcaacc ttaatcttaa tcttatcctt caaaggaata catcctatat tactttcact
    10441 ttttacatca acacttccaa caaatttacc agagtcatta taatactttg caacaccaac
    10501 aatatctccc ttatggatag gataattcat atttttaaac actaccttag gattgatatt
    10561 ctcgttatct gaaacaacac catacaaatc tttatcaacc ttaactgtta tcttaaaatc
    10621 atcaatacca tcaacacaag gagtctctat aatatcaccc ttatgtgcta tctttttaga
    10681 atggaaatta tctttaccat atttcaataa agtagcggaa gaagaaattc tatttttctt
    10741 gttaccatca ccaagtacaa cacctatcaa tctatgattt ctttcgtttt tagcatcttt
    10801 aggtaccatc atagaaaatg caaggcaata tccagcatta tctgtatatc ctgtctttat
    10861 tccatcaaca ccaggtactg aaacaagcag aggatttgtg ttataatgtg taaaatcctt
    10921 tttagtatca ttatatactt gcatagtggt tattcttgta acttgatttt cataatggtc
    10981 gtacatatac tttccaagtg taactatatc gtgagcagtt gacatatttt caataggagg
    11041 ttctttatgt tctgggtcag tatatatagg aagcccattt ggattaaaat aatacgtatc
    11101 aatcattcct atttcttctg ctttcttgtt catcatattt acaaaattct tttcagtttt
    11161 accaatatgt ttagctatgg ctatagcagc atcatttcca gatactagca ttagaccctg
    11221 cataagctct cctagagata tttcatcacc atctttaagt ttacaagtag aacctcctac
    11281 tgaagctgta gacttgtcta ttttcaccat atcagtctct ttaacttgat ttttatcaac
    11341 tgcttctatt gccaataaaa aggtcatcat tttacttaaa ctcgctaatg gcattttttc
    11401 atcaatgttt ttttcaaata atattctttt agtttcctgg tctataagta gtgctgctct
    11461 tgaagaattt ggtactggtg gagtatctgc aaatgcaaat acaggagtta taaatataaa
    11521 aattaatgca agtattgtta actgttttat ttttcctttc aaaatctcgt cctccatttc
    11581 atatattaat taatatgcac aaaataagtg ctataaattt tatttattta gatactcatt
    11641 aatcatgtct attatataag caatttcttc gtctggtatc aaaatattaa aataagcttc
    11701 tgttggctta aaggcttcac gtattaaact atatagttca tgatttttac taattaattc
    11761 agtaatgtta ttatatggaa aagttgtatt tttaaaacat ctttctatca taaaagagca
    11821 atgtagtata tatcctagct taaatttgct ctttaagttt tcatctatta attctattat
    11881 tgtaaataag gaagaattga catagttgaa taatttaaca gagtccaaaa gctctaagtt
    11941 ttgggtcata aagtttatta ttaaactgtt gtatgataga tttgtttctt ttatatttgt
    12001 aatactattg gcattgaatg taccttctat tattttgttt agcatatcta ggccatatcc
    12061 atttagtagt tcctctatgt gtataaaagg ggtgttggct acattaaaat ctacacttcc
    12121 aacaactgcc agaattttag tattactaaa tttattctta tacattttaa aactttctat
    12181 attatgagga actaaattaa tattgtattt actaatattt ttaagtgaag aatttataaa
    12241 gtctgctact ttcatagctg aaccatatcc tgttatacaa gtagttatta ttacatactc
    12301 tcttgacttt tcaggatagt ttatttctgt tgaatcgatt gctgctcgtc caatatatgg
    12361 atttaaagag cttaaggaat tagctaattc atccaaagtc atttctggta gtgtactttt
    12421 tcttactgct tctaaaacta ttggagtaga taccatttca attgagcgag ttttgatttt
    12481 tgttttttct gaaataagtt ctccaaaagc tataagagag cccatatctg ttaaaagaag
    12541 tacaccctta ccttcgtcac attcttttac caactctata gttttatcaa gtatggtact
    12601 tacttttaca tcaagaggca tgtcaattgc attacaatgg tttgtaccaa gcaatgaatt
    12661 tgctacatca cacatactgc ttgctgttga atttccatga gcaagtatta tgactcctat
    12721 actttcagat tgattgtttg gttcaagata catgctattt agaaacatgg atataaattt
    12781 tacttcatct ttaggtaaag atgtttgtaa gtcattttca attatactta aaactatttt
    12841 agctatattt aaatctttat ctaagaaaat tttatcatta tataaattgt acttaggatt
    12901 atatgtgtct tgatttatat tttctataag tgcacttata tgcattgaaa gagcaatata
    12961 agtttgtttt gtgtatttgt ttctcgatat tttttcagcc tcttttaaag aatgttcaac
    13021 agcatagtaa atctttggat tgattacttt aaaaatctca ttacttgtat aatcagaatg
    13081 agttactgaa gatatcgaaa gtaattttgt agagtacttt tccatgtaat ttataaacat
    13141 actccttata tcattttcca gataaggaat ctctgtagag tatttaaatc cattgtttga
    13201 tgtatctata ttgtatttag atagttcttt tgtatattca acttctaagt tattaccatt
    13261 aaattcataa aaaagcatat aatcaagttc tgataattta tttagattac tacgattttc
    13321 attcaatttt aaaagaccaa tatagatgta atcaggaaca atggataaat ctatttctat
    13381 attatcctta agtccagttt tgtaatttaa gaatgctctt gcactcataa gttgtatgtc
    13441 agatttaagc tgacctatat taccaaaaca atcataaagg agtaatgatt taattacatc
    13501 tttgtgaact tttatagttg cattggtgta ttttgattct ataataaaga agtatttaat
    13561 aagagataat ctttctttta aagacctatc ctgtaaagca ggtaggttga ttgtaacagg
    13621 gattcttctt aaaaaagtcc caagcagaga agaatgtata tcttctgttg tggcacacac
    13681 aataaagaca ttagctttat gcacaatgtc agattctcct aatcgtctgt atatgccttt
    13741 gtccatcaaa agaaaaagca tctcttgacc ttctggtggt aatctgtgta tttcatctaa
    13801 gaataatatg cctccatcag cctgtgcaac tatgccgaat ttatccttat tagcaccagt
    13861 aaaagaacct ttgacataac caaataaatt acttaataat aagttagcat tttctgcata
    13921 ttcagcacag ttaaatacta cgaatttgct ttcagaagaa aagattttac tttctacagc
    13981 atatttgtac attgtttcag caaatgtact ttttccaaca ccagtaggtc ctattagtaa
    14041 cgtattaaga ccttttggag gatagagtat agcagattta gctttttcta taggtagttt
    14101 aagactagaa tcatgtccaa gtaatgttga aaaaatatct tctgagtcat ttttacttga
    14161 tttaagtagc tcattacaat tatttataga aaattcatca ctgttcaaag tacggccaat
    14221 taaattttct ataatactag catgtagaaa taaaattggc ttaccagata ttttcagaac
    14281 ttttttttca ttgtataggt tatttaattc tttactggta ttacttctat caagagatga
    14341 atggttagca atttctattg tagtgaatcc tgttttatct gattgaaggt cttttaaagt
    14401 catattttca cataatttat gtagagataa tagaacttta tcttttctta gcatataccg
    14461 tttcctttct ttatgtttaa actatattta agctacatta aatatattat aatatatatt
    14521 cagtattaaa ataaggttta tagtggatat tttgtatttt actttttaat atgttgtttt
    14581 gtgtaataga tggtgttaaa ataagagttg cattatatta ggtatggatt tttatagatt
    14641 aaattagagt attatgttgt taaataacta aatttaagtt ttttaatgta cttaagtcta
    14701 tgtaatatca tttattaata tatggtcggt atagatgaa
//
LOCUS       DAEOSP010000093        14475 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.093, whole genome shotgun sequence.
ACCESSION   DAEOSP010000093 DAEOSP010000000
VERSION     DAEOSP010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14475
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.093"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            248..1444
                     /locus_tag="KRH54_002155"
     CDS             248..1444
                     /locus_tag="KRH54_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG8579812.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(1779..2231)
                     /locus_tag="KRH54_002156"
     CDS             complement(1779..2231)
                     /locus_tag="KRH54_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8579813.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(2542..3987)
                     /locus_tag="KRH54_002157"
     CDS             complement(2542..3987)
                     /locus_tag="KRH54_002157"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBG8579814.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(4131..5039)
                     /locus_tag="KRH54_002158"
     CDS             complement(4131..5039)
                     /locus_tag="KRH54_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG8579815.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            5296..6120
                     /locus_tag="KRH54_002159"
     CDS             5296..6120
                     /locus_tag="KRH54_002159"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG8579816.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            6160..6690
                     /gene="lepB"
                     /locus_tag="KRH54_002160"
     CDS             6160..6690
                     /gene="lepB"
                     /locus_tag="KRH54_002160"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBG8579817.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(6772..8553)
                     /locus_tag="KRH54_002161"
     CDS             complement(6772..8553)
                     /locus_tag="KRH54_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBG8579818.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(8935..11271)
                     /locus_tag="KRH54_002162"
     CDS             complement(8935..11271)
                     /locus_tag="KRH54_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG8579819.1"
                     /translation="MKKNTLLHTNIMVCIIITLGFVATSIIGYQSNTNIFKNDIEHVS
                     TLAAEGIYYQIDKLLSEPINVSLTMANDSLLKNFLDGEKEHLNDEEFIYKLQDYLNVY
                     RNKYSYDSVFLVSTKTNNYYHFNGLDRNLSANNSENQWYYTFLKNDDEYSLNVDNDEA
                     SNNSITVFVNCKIKDDNGATMGIIGVGLKVNSLQMLLKGYNDKFDVVARLIDDKGFVQ
                     LAVDKTGHENINFFENSSSDLSNSKQLILNNKKEQKSFWYSSEKSKSYIVCQYIPSLK
                     WHLVLENDFTLMIKQLHLQFFKGIAVIVLIIIFVLFTITHVLKKYKNQIIKLSVSQKL
                     EYQKLLSKSTEGLYENIFEIDITNNKAGCEDENRYFHEFGLGLNTPYDEALHTIAKNQ
                     IKEEYAQGYLDTFLPENVIKRYNSGINNISYDFLIKKDEESYHWIRINARIFFWTSDE
                     SVRMISYRKNIDEEKNRELLLLERSQRDSLTGLYNKATTEEFIRSFLKNESNQDLEHV
                     FLIFDIDNFKNVNDKYGHVFGDFVISEFALELKSQFREDDIVGRIGGDEFVVLIKDFN
                     NNNYVLIDKLERFCSKINQKKFVEQAEFSIACSIGVAMYPEHGYVYSELYEKADQALY
                     YAKSHGKNSYHIFSEDSIDSISFHIDSRDIKVLLEMATDGVVKFACMENLKLLYFNQK
                     MLEFTGYSAQTVTSIGFDIFSPVLQDDLQMVLDTLREAISKRETFEIVFRFCHTDGHI
                     FPVRMQGKFVSELYQNRYPVFYAIYTDISSINTLLENK"
     gene            complement(11585..13126)
                     /gene="rny"
                     /locus_tag="KRH54_002163"
     CDS             complement(11585..13126)
                     /gene="rny"
                     /locus_tag="KRH54_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBG8579820.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
     gene            complement(13302..14348)
                     /gene="recA"
                     /locus_tag="KRH54_002164"
     CDS             complement(13302..14348)
                     /gene="recA"
                     /locus_tag="KRH54_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBG8579821.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
BASE COUNT         4682 a         2316 c         1723 g         5754 t
ORIGIN      
        1 aaaaaatcac tatcagtaca tcagaatatt atcttactat aaattaattt atgaatacat
       61 aatttagtgg cataaacatg ggtattttac tataatttgt taaaagttta ttttaaagct
      121 atattaattt tttttgatga tttttaccca taagtatgtt attattatct tgtaaagatg
      181 tcttgtattc aaacatacaa tttacataat acaatatacg tatttaaatt aaataggggg
      241 tattaaaatg aattattcaa acagagttag tgcaatgcaa gcctctccaa tcagaaaact
      301 tgttccattt gcacaagcag caaaagataa aggaatcaaa gtatatcact taaacatagg
      361 acaaccagac ataaaaactc ctaaaggatt ttttgatgct gttaaaaact ttgatagcga
      421 agtcttagaa tatgctacat cagaaggtat tccagaatta ttagaagcac ttcaaaatta
      481 ctataaaact tacgatatga attttgaaaa agatgaatta ttagtaacta acggtggtag
      541 tgaagcttta cttttcacta tgatggcagt ttgtgaccca ggagataatc tattagttcc
      601 agaaccattc tatacaaact acaatggatt tggtcaatct gtaaacgtag aagttaatgc
      661 tgttacaaca aaggcagaaa acggtttcca ccttccatct aaagaagaaa tattgtctaa
      721 agtagatgat aaaactaagg ctataattct ttctaaccca ggaaatccaa ctggtgcaat
      781 ttatacaaaa gaagaactta atatcctagc tgaaatagct aaagaaaaag atttatggat
      841 aatagcagac gaagtttata gagaatttgt ttatgatggg cttgagtata caagctgtgg
      901 taacttagag ggtgtacagg atagagttat aataatagac agtgtttcta agagatacag
      961 tgcttgtggt gctagaattg gttctatcgc ttgcaaaaat aaaggtctta tcgctcaaat
     1021 acttaaatta tgtcaaggaa gactttgtgt tcctacatta gagcaaatag gagctgttga
     1081 gttatacaaa actcctgttt cttacttcaa agaagttaat gaagagtaca aaaaaagaag
     1141 agatgtatta tacaatgaat taatgaaggt tgaaggtgtt atatgtaaaa aaccaactgg
     1201 tgctttctat atagttgcta aattaccagt tgaaaatgca gaagatttca caatttggat
     1261 gttaaaagaa tttaataaag ataatgaaac agttatggtt tgccctgctg aaggattcta
     1321 tgctactcca ggtttaggac gtgatgaaat aagattagct tacatcttaa atgaaaaaga
     1381 ccttcataga gctgctacat tattaaaaga aggtttagag caatatgtag ctttacaaaa
     1441 ataatttttt aaacttaatt tatataaatt taatgatata aaatgtcccc ttttcagata
     1501 ttatttgaaa aggggacatt tcttaattaa tatattaatt aagacttaga cctgactatt
     1561 tttcattttc tttatatagt gttggccagt aatctttatt cactttaatt aaagcatcta
     1621 aaacttgtct tgccttagtt agagaaatta taaaaaagta catcaagaaa atttaagatg
     1681 tactttttag attgtattta gacttataat tatatagtta aataaataac aaagaattaa
     1741 tcttagtacc attaaataca aaaattttta tattttttct atctaaattc ttctttgttt
     1801 aatatatatt gaagagtatt tattgcttct aaatattttt ttctgtcttc ttcagttaat
     1861 ttagaaagtt tttctcctaa accatcactt aatttatcca taaaatcagt tgctatctta
     1921 tctccctctt cagtaatgtc aattagatat tttcttctat caactaaatc tcttttcctt
     1981 gttacatact tcttatcagt taaatcatca accatactag ttaaactgcc tttttctata
     2041 tttaacttag agcataaatc agtcatactt attactccat aattttttat gaaaactaat
     2101 gctcttaatt gtgttttatt tacattataa tctggtgcaa atttttttag gtcttctaag
     2161 tacaggctag aataaatctt aggaaatgtt tttactaaaa aacttatggt atcttttact
     2221 atatagttca tataatcacc tcggttgttt taaaatttat taaattatct aaataacttt
     2281 atttcagttt acattaaaaa tattgcaagt acaatagttt ttgctgaatg tttaaattta
     2341 attagtaatc ttatcatttt tttattttta tttatattat tcctattaaa tattatttat
     2401 atagctattt tattaaattc atggtatagt aagtaaagaa gaactcttca cagcggtgga
     2461 gagaagacgc tagcccatta aaatggctat cttatttata aataagatag ccgtttttaa
     2521 ttatatttta tctgtacaat tctatactct taggtctaca tctacttttc ctaagtcttc
     2581 cttataatct tttatagcag gttctacaac ttcatttaca aattctacaa cttgttctgg
     2641 agctctacct ataaagtttt ttgggtccat tatagatagt atttcttcct tgctcatttt
     2701 aaatgaatca tcattcatta taagttcaat taagttatta tccttgcctt catgctttac
     2761 tctataggca gctttcatag aatattctct tattatttca tgcaattcct gtctatctcc
     2821 acctcttttt acagcttcca taagtattgt ttctgtagcc ataaaaggaa gttcttcatt
     2881 tacacgtcta tttatcatat tttcatatac tactaaacca tcagttacat ttatccctat
     2941 ttcaagtata gcatctgctg ccataaatgc ttgaggtatt gccaatctct tattggctga
     3001 gtcatctaat gttctctcta accattgagt ggcttgtacc attgcagggc ttatagattc
     3061 agagattata tacttagata aagatgctat tctctcactt ctcattggat tcctcttata
     3121 agccattgct gatgaaccta tttgattttt ttcaaatggc tcttctaatt cttttaaact
     3181 ttgtaaaagt cttatatcat tagtcatctt atgcatactt tgagctatcc ctgatagtat
     3241 gcttattact tgataatcta actttctagt atatgtctgt ccacttactg cataggaaga
     3301 atggtatccc atctttttac aaattttctt atctaactct tttacttttt cgtggtctcc
     3361 ttcaaataag cttaaaaaac ttgcttgagt accagtagta cctttaactc ctcttaactt
     3421 catgttgtct attctatagt ttaaatcttc caaatcaaga attaaatctt gagcccataa
     3481 acaagctctt tttcctactg ttgtaagttg agcagcttgg aagtgtgtaa atccaagagc
     3541 tggcatatct ttgtacttaa gtgcaaattc ttttaaattg tttattaaat ttacaagctt
     3601 tactcttaga agttttaaag cttcattcat ttgtataaca tctgtattat ctccaacata
     3661 tgcagatgtt gctcctaaat gtattattcc tttggctttt tcacattgta aaccataagc
     3721 ttttacgtga gacattacat catgtcttac ttctttttct atttttctag cttcatcaaa
     3781 atttatatta tctatattag ctcttagctc atctaattgt tcttcagtta tattgattcc
     3841 taactcatgt tcaccttctg caagtgcaac ccataacctt ctccatgtag agaatttaaa
     3901 ttgtggagaa aatatataac tcatgtcctt gctacaatat ctgtctgtaa gtgggtttga
     3961 gtaagtagta tatttattat ctatcattat attagcacct tccttaaatc ttttgtcgtg
     4021 tgattactgt gtacacatta gtacataaat aattatatat aattttacga actataacaa
     4081 gactttttta ttttttgttc acatttatat atagtatttt ttatttatta ctatttttta
     4141 tttatatcca aattatcttt ttcaattaag taatccacta tttcactatc aacaaaattt
     4201 aatacatact tatatctaat tgcatagtat ttatcactcc atccaagctg attttttaca
     4261 tcactttcat ctgccccaag ccttaaactt attgctgcaa atgaatgtct aaaatctctt
     4321 gctgaatatt gagaaagtcc agctaaatct aatgcttttt tgactataag tctgacattt
     4381 ctatctgtta tagaattact cttttgagtt gtaaaaataa aatcatcact aggtattatt
     4441 acctctggaa gcccagaata attcctgtaa tcttctaaaa gtttaaaaaa gtatggatgt
     4501 aatttaacta ctctttcttt tcttccctta cctaacctta catatgagtt atcgttttca
     4561 tctaccataa ggtctttcca ctttaagcta actaattcat ttaataaaca cccagtagta
     4621 actagacaga ctattattac tctatctctt atatttaatt ttggtaggat acctataagt
     4681 ttatttattt cttgtatact tagaacatca tcttttgttt ttattcttgt tactgtgggt
     4741 ttttctacat atctaaatgg atttatatct atgtatcctt ctttttttaa gaagttataa
     4801 aaactatgta aataactata tatcttttca caagttgatt tcgcatattt agtctgtata
     4861 tagtctacaa aatttttaca atcttctttc gtagcataaa tcaattcttt cccttttaaa
     4921 aattctttaa acagtataac ctttgataaa taatcgtgtt tcgtgtttgg atttaatttt
     4981 gtagaaaaaa cattaaaagt ttcatactca taatcatcta gtatttttgt actatacata
     5041 ataactcctt tccttttcat aaaataaaat agctatactt ttactatacc atatatttcc
     5101 ccctcaaata taccccttat tcaatagata acttttaagt ttattttgtt atattatgaa
     5161 ggttttatga aaatatcaat cttccattgt gcactacaca gtactatggt attataagtt
     5221 agtaaaaggt ggcatttttt tattaatcta tgaataaaat atgctactat aaatttgtat
     5281 tggaggtatt ttgatttgga aacatttagt ttgcttgaaa tttttaaggc agtcattttg
     5341 ggcatagttc agggaataac agaatggcta ccagtaagta gtacagggca tatgatatta
     5401 gttgatgagt ttataaaatt gaatttttca aatacattta taagtacatt tttagttgta
     5461 attcaatttg gttcaatatt agctgtactt gttatatttt ttagaaaatt aaatcctttt
     5521 gatagcgcaa agaatattaa gcagaaaaaa gaaacagtcc gtttatggct taaggttata
     5581 attgcagtta taccatctgg agttattgga atactgtttg aagatgatat agatagatta
     5641 ttctttaatt caacagtagt tgctattgct cttattgtat atggtataat aatgataggg
     5701 cttgaaaaga gaaataaaaa acctaagtat aaagattttt ctcaagttac atataaatta
     5761 gctctttgta taggattatt ccagtgtcta gctcttatac caggtacatc taggtctggt
     5821 tctactataa taggtgcagt attacttggt acttctagat atgtagcagc agagttttca
     5881 ttcttcttag ccatccctac aatgcttgga gctagtgcat taaaacttct aaaagctgga
     5941 tttggattta ctggatttga atggcttatt ttaggtgttg gttctgtagt agcatttgtt
     6001 gtttcaattg tagttattaa attttttatg gactatatca aaaaacatga cttcaaagta
     6061 tttggttatt atagaatagt cttgggtata gtggttcttg cttatttctt tctattataa
     6121 tgtattctaa tttaataaca aatgataagg agaaaagtta tgagtgttaa aaaagaaata
     6181 tttgattgga ttaagtcaat agctatggct attgtacttg catttgtaat tctacaattt
     6241 ataagacctt ctattgtaag tggagaatca atgtatccta ctttagatga taaagattat
     6301 ctgattttaa ataggatatc atacaaggtt ggtaaacctg aaaaaggcga tattgtagtt
     6361 tttaaaacca atttagttga tggagaaaca ggaaagaaaa aagacttaat aaaaagagtt
     6421 atagctactg aaggtgacag aataaaaata tcaaattcta aagtgtatgt aaatggaaaa
     6481 ttattaaatg aaccatatat acacaataac tatacttctg gagatataga tactgttgtt
     6541 ccaaaaggta aactatttgc aatgggagat aatagagaaa atagtaatga tagtagattc
     6601 cctgatgtag gtatggttga tgaagatgaa gttcttggta aggttatggt gagactatta
     6661 cctcttgata atattgggaa agtagactaa aaaataagag actgaattgg taaagtttat
     6721 tttacaaatc tcagtctctt tttattttct taaattacaa atattttttt actaaatatc
     6781 ttttgattct gtaataagtc ttattatatc acttgcttct tcattaaaag ttgttactat
     6841 acttaggtag tctgttgctc tactcatagc taaataaacc ttatttaaat ctcctacaaa
     6901 gtcatttact tgataatctt gttcaatatc atttatagtt tgattgtata acatctctaa
     6961 ttcacatact attaccgctt tatactctaa attctttatt gcatatatat ttgctattgt
     7021 agttcctacc ttcttactta tatttgtcaa attatcttct gcacatatat aaggaatttt
     7081 tgcctcttcc aatgcttttt ttaacatata ttgaaagtaa atggtttttc cactttttaa
     7141 tttcttctta ttgtaaggat aaacgatagc aacttcagaa taatcaagac cttttttact
     7201 tattaaatac tcaatttccc agattacaga acttatttgg tcatcaaggt cacttacttt
     7261 tattatatca acagacttat ttccacctct aagtgcttta gtataaaaga aagtactaaa
     7321 ttctgtattt ggtcttaatt cgttgatata tgagttagaa ttatttgaaa atttatttgt
     7381 aaaatctaca atctctttag cttgtctata atttttactt aagataatct tatcatcaaa
     7441 ttctatacca ttatataatt ttttaaatat attcaatcta ttagagatat tcaaggagtt
     7501 acaagaacac acattaaata tgaattttgt tttatataaa aactccctta taaaatctat
     7561 ttcatactct aaaaagcttt ctgcttcatc aataaaaata gccttgaaca tatttttatt
     7621 ttttataatg ttctgtgctt gttttacaag attattaaag gtcttttcgt aatcattttt
     7681 taacatgtta taatctacaa ttaaattgaa ttttttagct agtttaaata taaaagaact
     7741 aaatgtgtga acttctaaat tgttattatc tttgtataat aactctatcc tctctcgaag
     7801 ctcattgcac agttgttttg tatgtgtaaa tatgactaat ttatgatgtg gatatacttt
     7861 tgctaatttt atagccctag aaagcataat tgctgttttg cctataccag aaccaccttc
     7921 aataagtgta ttcccatatt taatagaaac aacatcttta atttgaacgt cttccatcat
     7981 agttgctgta tacttatagt catcattgta gaatgaaatt tttttaaatt tttcatttag
     8041 atgaagttta tcatttatta tatagtattc tgaacatatg tctagtaaaa ataaatttaa
     8101 gtttatttca tcattctcat cattaagata ttcctccagc gaacccttat tcattatatc
     8161 ttgaagctta tttttatcta ttatattatt attaacaaat tcttcaaatt catatgtttc
     8221 ctcaacttca acgtatggca taatgtaaac gtagttataa cttatatttc tattcttttg
     8281 tcccattttt agtttcaaaa gttcatattc ctcattcata acttcaagta actcttcttc
     8341 taaaataaaa aatatatctt ctgttgtatc cataaatttt ataaataaaa ttcttccatc
     8401 tttaacatac aacaaatcgg tatttattcc tttaaaagga gtaacttttg gcactacttt
     8461 aacgtcctca ttgatatttt cgtaaaatat tttctcgaac tccttcaatt ttagggcgtc
     8521 taactgagaa tttatattta tgtattggct cacttacatt acctccttct gttatactta
     8581 attataccat tttgaactat atttttatat atacttttta taggcaaatt tagtaaataa
     8641 attcaaaata aatacaaata aatacaaaac tgctaaaaaa attttgcaat aagtatagat
     8701 atttattgtt ataaaatact ttctgtaaat aataaaatga taaatttcat aattaatgct
     8761 taaaataata agtaaagtct taaaaaaaat aaatcttgtt agccaaaaat aagatttatt
     8821 ataaaaaatt taaaaacctg ctaaaaataa tctattttca ttagcaggtt ttatttataa
     8881 tcttcttata ttaatactat tatatcctat ttttagattt tactgtagta tttactattt
     8941 attttctagt aaagtattga tacttgatat gtctgtataa atcgcataaa aaacaggata
     9001 acgattttga tataattcac ttacaaactt accttgcatc cttacaggaa aaatatgacc
     9061 atctgtatga caaaaacgaa aaactatttc aaaagtctct cttttagaaa ttgcttctct
     9121 taatgtatct agtaccattt gtaagtcgtc ttgtaataca ggcgaaaaaa tatcaaaacc
     9181 tatagaagta acagtttgag ctgaatatcc agtaaattca agcatttttt ggttgaaata
     9241 aagtaatttt aagttttcca tacaagcaaa tttgacaaca ccatcagtag ccatttctaa
     9301 taatactttt atatctctag agtctatatg aaatgaaata ctatcaatag aatcctctga
     9361 aaatatatga taagaatttt tcccatggct ttttgcatag taaagtgctt ggtctgcttt
     9421 ctcatatagt tcagagtata catatccatg ttcaggatac attgctacac caatactaca
     9481 tgcaatagaa aattctgctt gctcaacaaa ttttttctga ttaatcttag aacaaaaacg
     9541 ctctaatttg tctataagca catagttatt attattaaag tcttttatta atactacaaa
     9601 ctcatctcca ccaatacgtc ccacaatatc gtcctcacga aattgagatt ttaattctaa
     9661 ggcaaattca cttataacaa agtctccaaa tacatgacca tatttatcat ttacattttt
     9721 aaaattatca atatcgaaaa ttagaaaaac gtgttctaaa tcttgattac tttcattttt
     9781 taaaaatgaa cgaataaatt cctctgttgt agctttattg taaagccctg taagagaatc
     9841 acgttgtgaa cgttcgagaa gtagtaattc tctatttttt tcttcatcta tatttttacg
     9901 ataagatatc atacgaacag attcatctga tgtccaaaag aaaattcgtg cattaattct
     9961 aatccaatga taactttctt catctttttt aattaaaaaa tcatagctga tattattaat
    10021 tccgctatta taacgtttaa ttacattttc aggtaaaaat gtatctaaat aaccttgtgc
    10081 atattcttct ttgatttggt ttttagctat tgtatgtaag gcttcatcat atggggtatt
    10141 aagccctaat ccaaattcat gaaaatacct attctcatct tcacaaccag ctttattgtt
    10201 agtaatatca atttcaaaaa tattttcata aagaccttca gtagatttgc tcaacaattt
    10261 ttgatattcc aatttctgtg aaacactaag ctttattatt tggtttttgt attttttaag
    10321 cacatgtgta atagtaaaaa gtacaaatat tataattaaa actataactg caatgccttt
    10381 aaaaaattgc agatgaagct gtttaatcat taaagtaaaa tcattttcta gaacaagatg
    10441 ccattttaaa ctaggtatgt actgacatac aatataactt tttgattttt cagatgaata
    10501 ccaaaaactc ttttgttcct ttttgttgtt taatattaat tgtttcgaat tagacaagtc
    10561 gctactagaa ttttcaaaaa aatttatatt ctcatgaccg gttttatcta ctgctaattg
    10621 tacaaaccct ttatcatcaa ttaagcgtgc aacaacatca aatttgtcat tatatccttt
    10681 aagcaacatt tgtaatgaat taacctttaa cccaacacct ataattccca tagttgctcc
    10741 attatcatct tttatcttac aatttacaaa gactgtaata ctattgttac ttgcttcatc
    10801 attatcaaca tttaatgagt attcatcatc attctttaag aaagtataat accattgatt
    10861 ctctgagtta ttagcgctaa gatttctatc aagtccatta aaatgataat aattattggt
    10921 tttagttgat actaaaaaaa cagaatcata tgaatattta tttctataca cattcagata
    10981 atcttgtaat ttataaataa attcttcatc atttaaatgt tctttctcac catctaaaaa
    11041 attttttaat agactgtcat ttgccattgt aagagataca tttataggtt ctgaaagtag
    11101 tttatctatc tgataataaa taccttcagc agccaatgta gatacatgtt caatatcatt
    11161 tttaaaaata ttggtattag attgatatcc aattatagag gtagccacaa aaccaagagt
    11221 aataataatg caaaccataa tatttgtatg taaaagtgta ttttttttca tagtgtaccc
    11281 tctctctaat cctcacttta gcgtcattgt attttcatct tatttaagta caagcattcc
    11341 aagtagtaat atatttattt tacaacttca ttatatcaaa caaacttgga taattatata
    11401 aataatacat atataaagct ttattttatt aatcttttgt aagtagcact tttagcactt
    11461 agattatttt tgcattatat aaaattaaac ttaaacaatt taaaagccac aagatatatt
    11521 atcatatctt gtggcaaatt taaatttcta aaaatatttt cttattattt agatattatt
    11581 tcttttactt cgcatactct atagctcttg tttctcttat tatgcttact tttatttgtc
    11641 caggatattc aagttcatcc tctattttct tagtcatatc tctagccaat agatgtattt
    11701 cttcatcatt tatagcttct ggttttacca ttattcttat ttctctacca gcttgtatag
    11761 caaatgattt ttctacacct tcatatgaat tagctatttc ttctaatttt tcaagtctct
    11821 ttatatatgc ttctaaagtt tctcttctcg caccaggtct agcagctgat attgcatctg
    11881 ctgctgtaac taatacagcc tctactgttt gaggctcgta atctccatga tgagtactca
    11941 tggcatgtat aacttcttta gactctttat aacgtctaag taaatccata cctatttcaa
    12001 catgtgtacc ttccatttca tggtctacag ccttacctat atcatgtaat aaacctgccc
    12061 tcttagccaa tctaatatct gctcctattt cagcagccat tataccagct atatgagcaa
    12121 cttctataga atgtttaagt acattttgtc cataactagt tctatagtta agtctaccta
    12181 ataatcttat taattccgga tgaatcccat gtacactagt ttcaaatgca gcttgttcac
    12241 catattcttt tataatattg tcaacttctt ttctcgcctt ttcaaccatt tcttcaattc
    12301 tagctggatg tattcttcca tctgctatta atttttctag agcaattctt gcaatttctc
    12361 ttcttattgg atcaaatcct gataaaatta cggcttctgg agtgtcatct attataaggt
    12421 ctatacctgt taaggtttct aaagtcctta tatttctacc ttccctacct attattctac
    12481 ctttcatttc atcattaggc aagcttacta ctgttacagt agtttcggct acgtgatctg
    12541 ctgcacattt ttgaattgca tatcctataa tttctcttga tttcttatca gcttcttctt
    12601 tagcttgact ttctatctct tttatcatta tagacatttc acgtcttaca tctctctccg
    12661 cattggttaa gatgatttct ttggccttat cagaagtaat tcctgatata ctttctaatt
    12721 tttctaactg ttgagttttt attacttcaa cttcttcttc tttcttttcc actaccttta
    12781 atttttcact taggtttact tctttagatt ctaaattttg taattttcta tctaagactt
    12841 cttctttttg tactactctt ttttcatact tttgtacttc tgttcttctt tctctgtttt
    12901 ctctttcagc ttctgttctc cacttatgga tctcttcttt agcttctagt aatttttctt
    12961 tttgtactgt ttctgagtcg tggtgtgctt tatctattat ttctttggcc aagttttcag
    13021 cttgtccaat ttttgcttca gatatatttt ttctaacaaa atatccagct ataataccaa
    13081 cagctgcacc tattacaatc attactacac tatctatgac atccacctcc ttcgttaata
    13141 atcccaaaat gtcatcacta tgattaaaaa atattcctaa taattaaaat taaatttaga
    13201 tattttatcc tcacttaatt ttataatttt ttcaacattg tgtcaagcta atagggagcg
    13261 tggcatgtct ttaattacta gttatttttt tgtattttta cttattcttc tacaatttct
    13321 tttgatactg aagtacctga ttcttcatgt tcttcattta aattgtaaaa tgctctaact
    13381 ttctcatcta tttcagtagt taaatctaaa ttatcttcca agaatttttt aacattttct
    13441 cttccttgtc caagtttagt atcattgtaa ctataccatg aacctgattt tttaactata
    13501 tctacatcag cagctatatc taaaaggtct ccaatttttg aaatcccttc tccatacatt
    13561 atatcaaatt cagcttgctt aaatggtggt gctactttgt ttttaacaac tttaactcta
    13621 gttctactac ctataacttt atcaccttgt tttattgtat ctatttttct aacatccaat
    13681 ctaactgatg aatagaattt tagtgcacgt cctccagtag tagtttctgg gtttccaaac
    13741 attattccta ctttctctct taactggttt ataaatatag caacacaatt tgatttttta
    13801 attgaaccag ttaactttct aagtgcttgt gacataagtc tagcttgtaa acctacatga
    13861 gaatcaccca tatctccatc tatttcagcc tttggaacta atgctgctac tgagtctatt
    13921 actattatat ctatcgctcc acttcttatc aatgcctctg ctatctctaa agcctgttca
    13981 cctgtatctg gttgagatat tattaggtta tcaacatcca cacccaaagc ttttgcatat
    14041 actgggtcaa gtgcatgttc tgcatctata aatgcagcta ttcctccatc tttttgagct
    14101 gatgctacac aactaagcgc aacagtagtc ttaccagaag attctggacc atatacttca
    14161 actattctcc ctctaggtag acctcctata ccaatagcta tgtctaaacc aatcgctcct
    14221 gttgatataa catctataga catagatgtt gcttctccca atttcattac tgaaccttta
    14281 ccaaaatctt tttcaatttt acctaaagct tcattcaacg cttttaattt ttcttgatct
    14341 acactcatct ctataccatc ccttcattta tctacaaaca tttgttctct tgttatatat
    14401 taattatata acataaatca aacctagtca atttaaaaac gctactaatg attatatttt
    14461 tattcattac atttt
//
LOCUS       DAEOSP010000094        14421 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.094, whole genome shotgun sequence.
ACCESSION   DAEOSP010000094 DAEOSP010000000
VERSION     DAEOSP010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14421
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.094"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            112..1023
                     /locus_tag="KRH54_002165"
     CDS             112..1023
                     /locus_tag="KRH54_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG8579822.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            1198..1695
                     /locus_tag="KRH54_002166"
     CDS             1198..1695
                     /locus_tag="KRH54_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBG8579823.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
     gene            2439..2801
                     /locus_tag="KRH54_002167"
     CDS             2439..2801
                     /locus_tag="KRH54_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG8579824.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            2866..3483
                     /locus_tag="KRH54_002168"
     CDS             2866..3483
                     /locus_tag="KRH54_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBG8579825.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLKEILVRLGLEV"
     gene            3480..3965
                     /locus_tag="KRH54_002169"
     CDS             3480..3965
                     /locus_tag="KRH54_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBG8579826.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            4008..4526
                     /locus_tag="KRH54_002170"
     CDS             4008..4526
                     /locus_tag="KRH54_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBG8579827.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKIIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            4662..5621
                     /locus_tag="KRH54_002171"
     CDS             4662..5621
                     /locus_tag="KRH54_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG8579828.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            5647..7350
                     /locus_tag="KRH54_002172"
     CDS             5647..7350
                     /locus_tag="KRH54_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBG8579829.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            7563..9665
                     /locus_tag="KRH54_002173"
     CDS             7563..9665
                     /locus_tag="KRH54_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBG8579830.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESTDEKEVIDSNFEYKEG
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            9678..10118
                     /locus_tag="KRH54_002174"
     CDS             9678..10118
                     /locus_tag="KRH54_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBG8579831.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            10154..10957
                     /locus_tag="KRH54_002175"
     CDS             10154..10957
                     /locus_tag="KRH54_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBG8579832.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            10997..11593
                     /locus_tag="KRH54_002176"
     CDS             10997..11593
                     /locus_tag="KRH54_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBG8579833.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            11699..12562
                     /locus_tag="KRH54_002177"
     CDS             11699..12562
                     /locus_tag="KRH54_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBG8579834.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            12629..13618
                     /locus_tag="KRH54_002178"
     CDS             12629..13618
                     /locus_tag="KRH54_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBG8579835.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            13710..14321
                     /locus_tag="KRH54_002179"
     CDS             13710..14321
                     /locus_tag="KRH54_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG8579836.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         5866 a         1362 c         2671 g         4522 t
ORIGIN      
        1 ataaaatcat gtaaaaataa tttagataag attctaagat tttatttatt taaaaattag
       61 tatgattaat ttagattaaa taaatttaat aattttagta ggtggtaatg aatgaaaata
      121 aatagattaa cagaaataat agtaattcta ttaaataaaa aattagtaac agctaaagag
      181 ttggcagata gatttgaagt ttctacaaga accatatata gagatataga aactctgtct
      241 atgtcgggag taccagtata tatgacgaaa ggcaaaggag gagggatatc tttaatagaa
      301 gaatattcta tagacaaggc tatattgtca aaaaaagata aagagagttt aatagttgca
      361 ctgaaaacat tacaggcgac taagtatcca gaaataaatt cagtagtaaa taaaattggc
      421 tcaatatttg gagagcagaa tttttcaaac tggattgaaa tagactttac tgaatgggga
      481 agtaatttta atgaagatga taaatttaca aaaataaaag aagctatttt aaggcgtaat
      541 actataaatt ttaattatgt aaatagtttg tcaagtcaga caaacagaac tgtcgaacca
      601 ctgaagctta tgtataagag taagacttgg tacttgtatg gattttgtaa actaaaagat
      661 gattttagga tatttagaat tagtaggata aggaatttat ctataaagga tgaagtattt
      721 tcaagaaaga taatagaaga agtatgtttg aatgattcta aagtaattaa ggaaaatact
      781 ataacgttga aattgaggtt taaagaaaag atgcttttta gagtatttga tgattttaat
      841 aaggatttaa taactaaaaa tgaagatgat acatatgatg taattacaga atttcctatt
      901 ggtgaatgga tttatggata tattttatca tttggtgaca atgttgaagt acttgaacct
      961 aaagatgtta gggataatgt aataactaga ctaagggagt tgtctaaaat ttattcatta
     1021 taatataatg ctagattaaa gattttatta aatatataat atcttgcatt tagtatataa
     1081 tatctcactg agtttaatta tattaaaaat atgacataaa ggtgtcgtat attataaatt
     1141 ataattgaat tagttcaaaa taaaatagat taataaaaac aaagtgaggt ataaagtatg
     1201 aattatgaaa tagtagagat aaatgagaaa atagttatag gaatatcgaa ggagactaca
     1261 aataaagatg gacaagcggt aaatgatata ggtgaattgt ggaaaaaatt tatgggcaaa
     1321 gggatatata atgctataaa gggtaaaaag aatgacaaga caataggtct atatacagat
     1381 taccagggtg attttacaag tccgtatagc tttgttgcat gttgtgaagt aaatagtaat
     1441 tctgataaag agaagaattt agaagaatta aacactaata atactgttgg agaaagtatt
     1501 attagtaaag ttataccagc aggtaagtat gcaaagtttg ttatagtagg aggtcaaaaa
     1561 gaagtaggag atttttggtt tgagttttgg caaatggatt ttgataggac ttatataagt
     1621 gattttgaag agtatcaatg taatactttt gatacaaaga aacaagaaat tcatatttac
     1681 ataggaataa agtagatttg ttattactat attgaatgaa atattgagta tagtacattt
     1741 attatagata gttttattat ttataactat aagcagtaaa tctgtaaaag atttactgct
     1801 tttttagtaa ttaatagtat cagtatcact ttgaatcttc agacatattc ttcatttaat
     1861 attgtttatt gataaatgat acttgtaaat ataatgattt gttatttaat ttaaaatatg
     1921 aaaaaaagta ttataaacac attagaatac ttctggattt atgagtatac aatttgaaaa
     1981 atataaaaaa taatttataa agctaaatct aatttaatcg ataatacata tagagaaata
     2041 taattaagta aataaacaac tatctattag atattgagaa agtcaaatcc tatatgaatc
     2101 tacagaaatg attttaatga atctgaaaat tttttttata caaacataaa aaaggtataa
     2161 agttgtttta ttataattta attacttttg ataacttttt atgttttata caattatatt
     2221 tttaataact cgtcaaaacg ccaaacatat attacaatag atatataagt ttttttgttg
     2281 acaaattatg ttaagagaat taagtataat atggcataaa taattacttt tatttatcaa
     2341 tgaaatgtat ttattgtgta gcgaaaaata gtcagatagt atagacaata actgaagata
     2401 aaagcgttta ttgttgtaca tatggaggag ttagttttat ggaaaataaa attttgatag
     2461 tagatgatgc tatttttatg agaatgataa ttaaagaagc tctgagtaaa agtggctata
     2521 ataatttaat agaagcaaat gatggagaag aagcgtgtaa tatatttaaa tatgaaaagc
     2581 ctgatttgat gttgcttgat attacaatgc caaaaaaaga tggtttagag gtgctaagag
     2641 aaataaaaaa gttggattca agtgctaagg ttgtaatgtg ctctgcaata ggacaagaga
     2701 atatgattgt agaagccata aaacttggag cgttagattt tatagtgaag cccttcaaaa
     2761 cagaaacatt agttaaagtt gtagataatg ctttaaaata aaagtgtaaa tatacactaa
     2821 tacaatgaaa agcaaataaa tgcaataata aagggagggg tttatattat aaattattta
     2881 gaacttgatg atgttcatat agatgctctt agagaaattg gtaatatagg ctctgggaat
     2941 gcaattactg cacttgcatc catgataaat agcaatgtag aagtattaat acctatggtc
     3001 aagatacttg aatataatga agcgacaaat ttgcttggtg gtccagaaaa caaagtagta
     3061 tgtattttac tcgatatgaa aggtgatatc aatggaatgt ttatgttttt gttggatgaa
     3121 tcaataactc aacttatgct aagttctctt tttaataaag aagaagcttt tttagatgag
     3181 attgaggcaa ttgaaatttc agcaattaag gaaataggaa atattatggc aagttcatat
     3241 gtaaatgcta ttgcatctat gttaaacatg actataagtg tttcaattcc agatatatgt
     3301 attgatatgg ttggagctgt tctaaatgta cctatgataa gattttcaga tgttggtgac
     3361 aaagtcttat ttatagaaaa taaatttaaa atgagtgata attattttac tagtcacatt
     3421 ttgatgattc cagaaatgag ctcacttaaa gagatactcg tgaggttggg attagaggta
     3481 tgaatagaga gatagtagtg aacattgcag atatgaagat agcctataga ccaaatgttt
     3541 tagttactta tgcacttggt tcttgtgtag gagtatgttt gatagataaa gtagctggaa
     3601 ttggtggaat gttacacgtt atgttgccat atagtaaaga tgctattaac attgaaaata
     3661 agtgtaaatt tgctgacaca ggaataaatg agctcattaa atctatggag aatataggtg
     3721 caaatagaat atatataaaa gcaaaaattg ctggaggtgc tcagatgttt ttaggaagta
     3781 gcaattcagt aattgcaagt ataggacata ggaatgttga ggcagtaaaa aaaactcttt
     3841 taggattaaa tattccaata ttagctgagg atacaggtat gaattatggt agaacaatac
     3901 gtttttacac tgagacgggt gaattacttg tagattcaat aaataaaggt aagaagaaat
     3961 tataatatat atttcataga aagtgtattt taggagggag gtattaaatg agatttccag
     4021 atgacccata tttagattat ggactaattg atgaaggaat taagtatcta aagtttaaag
     4081 tagatgagca agattttggg gttgaacttg aaaagataat agaaattact ggaaatcaag
     4141 aaattatatt tattccagaa ctacctacat attctaaagg cataataaat ctaagaggaa
     4201 aaatcattcc gataatagat atgcgtttga gacttaaaaa agatgaatta atgactacaa
     4261 attgcatgta tatagtagta actgaaataa aggacttggt agttggattt gtggttgata
     4321 gagttgatga tatagtaagc ttagaaaaaa gtaaaatatc acctccacca agagttacta
     4381 tagaatattc ggattatttt gtaagtggag ttggagaatt agaaggtaaa ataattactt
     4441 tactagattt agagaaactt ttgacagata aagatgaagt tttaatagat gaattaatca
     4501 atgggtttga ttgcactatc atgtaatata gggatgtagt tggagtgtta ttataaaaag
     4561 attgtgatga gaacatttaa gaatatagtg gtataattat taagtataaa aattttaaat
     4621 cattaaaatt tgaatatatt atgtttaaga gaggtttgta tatgaaaaat aagcaacagg
     4681 gaacaatttt agtcgttgat gatagtgttc tgatgtgtga cttaatagat aaaacactta
     4741 caaaagataa ttttaaggtt aaaaaagcat ttaatgcaat agaggcaaaa gaatttattg
     4801 aagaatttat accagacatt attttgcttg atataatttt accagataat tctggttttg
     4861 agttttgtaa ggaagtaaaa tctaatgctc gtacagcaga tatacctata atttttataa
     4921 cttcaaaaaa tacagataca gacatagtaa aaggatttgg tgtaggagca attgactata
     4981 tggtgaaacc ttttagtatg acagaattaa aagctcgtgt tatagctcat cttgaagtaa
     5041 agaaatctca agataaacta aaaaagataa ataatgaact ggaatcttct cttgaaaaat
     5101 tgaatagact cgttgtaaga gattatctta caggtcttta caataggcga catataatta
     5161 atcagcttat ggagcaaaga agaattaata aatatagtga aacatcaata tcccttatcc
     5221 ttggtgatat tgatgacttt aaggttatca atgatactta tgggcatgaa gcaggggatt
     5281 tcgtgttaac agtaatatca agtataataa aaaagaattg tagacaaatt gatactgtat
     5341 caagatgggg tggagaagag tttttgataa tgctcacaaa tactccaata gaaggtgcta
     5401 agatactttc agaaagaatc cgtaaagaga taaaggagtt tgatttcaac tataaagaac
     5461 ataaaataaa gtgcactatt acattaggag tagtagaagt aaactcaaat atatcattag
     5521 aagaaaacat atccttagca gataaagcaa tgtatgaggg taaaaactta ggaaaagatt
     5581 gtagcgtagt gagtacaatg aaaggattaa aaaagatata aacttacttt atgaaggagt
     5641 acagatatga aaagagaagt aaagctttct aaattagcaa ttgtattgct gattattata
     5701 gtattggctg ttctagcagg gattttttct tctatttcat cttataaagc atttgaaaaa
     5761 gtagaaaaaa gtattaatga tatgaataat ttgaggactc aaagtcagta tataaaagat
     5821 acaactagag agataagtga tgtattaaga agatatgtat ttacaggaga aaaaaagaat
     5881 aaagatattt atgaaaaaga attatacgga aaaacaagag aatcagcttt aaacaaatta
     5941 gaaaaaattg gtcttacaaa gagcgagtca aataaaatct tagaagcaaa agaaatatca
     6001 gattctttag taccaacaga acaaagtgca ataaaggcag tcgaaagtaa tgataagagt
     6061 aaagctcaaa gtataatttt tggtagtgac tatataaact cccaagagaa aatggattct
     6121 ttaataaatg aatttcaaga ggatatagtt acccgttcaa attccaaaat tgtagaagta
     6181 aaaaaagaat tacaaagtct aatattaaga acaatattta tactttccct tttaataata
     6241 ttgacaataa tagaatatac agtaataaaa ttaaaaatag tgattcctat taataaagta
     6301 gaaaaacact tttcaagaat tgcatctggg gacttaagaa caagtataga cgttgaagaa
     6361 agtaaatctg aaataggact attagtgagt tctgtcaaaa agatgcaaag tatgctttta
     6421 aattacatag accttataga cagtacatta actagtattg caaaaggaga tttaagagta
     6481 gagatagacc aagaatttat aggtgatttt aaatcaatta aaatatcttt agaaagtata
     6541 ataaactctt ttaatggtga tttttctgaa ataaatttat cagcagagca agttgcaagt
     6601 gcttcagaac aagttgcagc aggtgcacaa gctctttcac agggtgctac tgaacaagct
     6661 agttcagtag aagaattagc ttccacgatt aatgagatat cagatggaat taagaagtgt
     6721 gcagatagtg ctagtcttgc atgtgaggta tctgaccaat ctgcatcaca agttggtttc
     6781 ggaagtagat gtatggatga tatgatgaca tcaatgggtg aaataagtga aaaatcgagt
     6841 gaaatatcaa aaataatcaa aacaatagat gagatagcat tccaaactaa tattcttgca
     6901 ttaaatgcag ctgttgaagc agcaagagca ggtcaatatg gtagaggatt tgcagttgta
     6961 gcagatgaag ttaggtcttt ggctggtaaa agtgcaaaag cagctcaaaa tacagctatt
     7021 ctcatagaag aagctattaa aacagtagaa aatggtgtca gaattgctaa agagactgca
     7081 aatgcattgg acttggtagt aaatggtgta gaaaaaacta ctaattatat taatggtatt
     7141 tcttcggctg tacatagtca agaagtagca attacacaga ttttatctgg aatagagcaa
     7201 atttccatag tagtccaaat gaactcagca acagcagagg aaagtgcagc agcctcagaa
     7261 gagatgtctg ctcaagctca aattttaaaa gaattagtag aaaaatttca tctaaaggaa
     7321 agtgaagtaa gagaatcatc atgggtttaa attatgaggt gaatattgaa gtaagagaat
     7381 tatcatgtat ttaaattatg agatgtatat atattgaagt aagagaataa ttgtgtattt
     7441 aaattatgag gtacatatag tgagataaga gaatcatcaa ggatttaaat tatgagatgc
     7501 atatataata gaaaattatt tgtaggtcgt aggtgataaa tgtgaattat attgaaccga
     7561 gtatgagaca aatgttagat atgtttatat ttgagacaag tttattactc gaacaattag
     7621 atgaaattat gattcaaata gaaaaagaga aagtatttac tcataacaac attaatgaaa
     7681 cttttagaat aatgcatacg ataaaaggtt cagcatctat gatggggtta gacaatatat
     7741 ctgctttagc acatagtgta gaagacttgt tttatgctat acgtgaaggt aaaatagaag
     7801 ttttagacaa tgacctatta tttgatttag tctttcaatc attagatttt ataagaagag
     7861 atatagatga gttacaatca agaggttgta tatcttctga ttactctaat tttattgaag
     7921 gaattgaaga gtatgtgagt actttggaat tatcaagttc tatgaaatca aaggataatg
     7981 caaatttaga aagtacagac gaaaaagaag taatagattc aaattttgaa tacaaagaag
     8041 gctctgatgt agtaaaaata atctttgaac aaggttgcat gatggaaaat gtgagagctt
     8101 tcatgcttgt atttgaactt agagattact gtgaatttat agatttttat ccaaaagatg
     8161 tagaaacaaa tgcagagtct acagagtata ttaaaagtta tgggttttat atacaatttg
     8221 tagctaaaga ggataaggca atagtttata aaactataga agataatctc tatgtaaaag
     8281 aatacataaa tattagtaaa aaagaaatag aaatgaaaaa tacatcaaac aatgatgcag
     8341 tagaaaataa attaaaagat aataataaag taagtaaaag ttatcaaaat aactatacag
     8401 aagatgagat acaagaagat gttttaaatg aaaaaactga tatggaaaaa gatagtctaa
     8461 gtgaagaaaa tgaacattta ccaataagta agcagagtat tatgagtgta aatttagaaa
     8521 aattggactt acttcaaaat atagttggag aaattgtaat tatggaatca atggttgtaa
     8581 atagcgtaaa ttctacaggt gttgatatag ataatttcaa taaggcaaca agacaattac
     8641 acaagcttac agatgaattg caagatatag taatgtctat gcgtatgatt caaattgctg
     8701 gtgtatttca aaaaatgcat agaattgtac gagatatgaa taaaaaatta gataaaaatg
     8761 tagagttaat aactatagga gcagagacag aggtagataa gagtgtaatt gataatttag
     8821 cagaaccttt tatgcacctt ataagaaatg ctatggacca tgcaatagag cctattgagg
     8881 agcgtatagc gaaaggtaaa gaagaagtgg gaaaaatagt tttatctgct gaaaatctag
     8941 gcggagaagc tgtgataagt atttcagatg atggtagagg cattatgaga gataagattt
     9001 tggcaaaagc taaggaacaa ggtcttctta aacatcaaag taatgagtat actgatgacg
     9061 aggtcaattc tttgattatg actccaggct tttctacaaa agaatctata actgaatttt
     9121 ctggaagagg agtaggcatg gacattgttc gtaaaagtat tgaaaaagtt ggaggttcaa
     9181 ttgaagtaaa aagtaaacag gatgaaggga caacctttac tataaaaata ccattaactc
     9241 tttctatagt agagggtatg aagtttaaag ttggagattc actatttact ttaccaatat
     9301 catccataag aagaacattt aaacttacag acttaaatca aataattaca gaaggaaata
     9361 agacagagtt aattacaata tatggagcat gtattcccat tataaggtta cataaaatat
     9421 ataatattca aacaaaagta accaatctga tgaatggtaa tatgataatt attgaaaatg
     9481 agcataaagc agtatgcatt tttgttgatg aaataatagg taatcagcaa gttgttgtga
     9541 aaccatttcc agtttatttt aaccgattta atataaaaaa taagggttta tcgggatgta
     9601 caatacttgg ggatggaagt ataagtttaa ttatagatgc agatagcatt atagaaatat
     9661 attaggggat ttaaagtatg gctaaagatg aaattattaa acaagtatta acattttatg
     9721 tcaatgatgt tatttatgga atagaattag aaaatgtgat tgaaaccata agatttcaat
     9781 caataacgta tgtaccatgt cttccaacat atataagtgg agttattaat ttgagaggaa
     9841 gaataatacc agttattaat atgcatataa agtacaatct tcctaaagct gattataatg
     9901 agagaacttg tataataatt acaaaggttg atgagtatca agttggaatt attgtagata
     9961 aagttgttga tgttattcat atagaaaatc taaatttact agagacgact aattctaaca
    10021 acactaatat taataaagat ataaaagaga ttgcgaaagt tgaagataat tctatattaa
    10081 ttttagatat tcgaaagttt ttgattaata gcatatagtt agttctgata aattagcagg
    10141 aggaagtttt tgcatgataa aactcacaga tgaagaattt atagttcttg taaaccatgt
    10201 aaaaaaagaa tatggtattg atttaagtaa aaaaagagcg ttaattgaag gtcgcttata
    10261 caatacaatg atagagaaaa aacttagttc ttttagtcaa tatatgaatc tattatttaa
    10321 agataagaca ggaaatgaag ctataaacct aataaataga ttgagtacta accacacatt
    10381 ttttatgaga gaacctcaac attttgagtt tatacagaat agtatacttc ctttttggga
    10441 agaaaataat aaaataagaa acataaatat atggagtgca ggatgttcta gtggagaaga
    10501 agcgtattca atagctatga ctttggatga ttattttgga tataataaag agttgtggaa
    10561 cattaaaatc atagccacag atatttcaac taactcactt gaaaaagcta aaagaggtat
    10621 atacatagaa tcaaatgtta acaatgttcc tgagttatgg aagaagaagt attttataga
    10681 caacaaggat ggtacattta aaatttgtga taagattaga aaaagtgtaa tttttaagcc
    10741 gtttaattta atgaatgatt tctcatatca acattttgat ttgatttttt gtagaaatgt
    10801 tatgatttat tttgatttaa aaacaagaga agaacttata aataaatttt ataatgctac
    10861 caaaaaagat agttttttgt ttgtaggaca tgcagaagta atcaacagaa gtagtactaa
    10921 atacaaatat gtaaaacctg ctatatataa acgataaaaa ggtatttttg atatatgatt
    10981 ttttggaggc ggtaatgtgg aacgttttaa taatatggtt attgctatag gcgcatctgt
    11041 aggtggtaca gaggcaatac ttgaaattat aaaagaccta ccaaaatcaa caccagggat
    11101 tgttgtagtt caacatatgc cagctatatt tacttatatg tatgcacaac ggcttgacaa
    11161 gcagtgtatt atgaatgtaa gagaagctaa gaataatgat agagtagaac aaggaaatgt
    11221 gttaattgca cctgggggtt accagatgaa attgtgtacg gataaacaag gatattatgt
    11281 aacttgtgaa aaaggagaaa gaataagtgg tcactgtcca tcagtagacg ttttgtttga
    11341 ttcagtagca gaagtagcag gtaaaaattc aataggaata atacttacag gtatgggtta
    11401 tgatggagca agtggacttt taaaaatgaa agaaaatggt tcatttacaa ttggacagga
    11461 tgaaaattct tgtatagtat atggaatgcc aatggtagct tttgatagag gttctgttat
    11521 gttacaatta cctcttggtg aaatttcaaa ctgtttgata agacatatta atattttaaa
    11581 acaaaaagat taatttttta taaataaaaa ttaatctttt attaaattat atggataaaa
    11641 aaccctagaa actccaaata taataagaga gatttatatt taggaggaaa atgataatat
    11701 gaaatttttt aaagtcgata caaaagctaa agttttaaga tatgtatttg ttttaacatt
    11761 gactataatg gctgttttgg caattaaaca tgactataaa tataataatt taaggaaaga
    11821 tgcttcaatt aatttaaata gtaataagga agcagtgact gttatgagta accagtcatt
    11881 accaaagggt aatacaaata gtaaacaaac tttatcagta agtacaatag gaagtaaaac
    11941 aatagtgaaa aatgatgagt atttagaaag tactataaac atgcctgtaa taacaaattc
    12001 aaataaaatt gttgaaaggt ctacaaatga taagataaaa aatgacatat ttgaatttta
    12061 taataaatca tataaagaag caaagcagta tcttaaggat aatcctgacg agaaaaataa
    12121 gtttgttgca aatgtagact ttgaacttaa aaagaatact gattctgctc ttagtataaa
    12181 agtgagatat tatacttata gtggtggagc acatggattt tatcaagata tagcatataa
    12241 tgtggatatg agaactggaa aatttttaga attaatggac ttgtttaagg ataatactaa
    12301 atataaagaa gttatagatg aagaaattaa aagacaaata gctgaacttg aaaaaaagga
    12361 tgaagaaaat atcggaatat ataattttaa aggtataaaa gagaatcaaa acttttactt
    12421 gcaagatgag aatctagttg tatattttga tttatatgat ataactccat atgcagcagg
    12481 aataccagaa ttttcaataa ataaagcgtt gataagttct atgctaaaac cagagtatgt
    12541 agatttattt gatttgaaat agaagattaa attaaatgtt atgctataat atttagcata
    12601 gagacttatt aaaattgggg gtataaaaat gctactttta aaaaaagacg acataaaaaa
    12661 agtatttaca atgagagatg caattgaagc tgataaggaa gcttttagaa tttattgtga
    12721 aggaaagagt gtaaatcctt tgagaaccaa tatatctgtt ccaagtcaag atgctagtat
    12781 gttatttatg ccaggatatg tagaagaatt aggctgtggg ggaataaaaa ttgtatctgt
    12841 atttcctaaa aatgctcaaa aaggcaaacc agtaattcca gcaacagtac tattattaga
    12901 tggagaaact ggagaagtta gtgcagtatt ggatggtact tatgtaactc agattcgtac
    12961 tggagcagcc tctggagcag ctatagatgt attggctaaa aaagattcta agattggagc
    13021 tttgataggt ctaggaggac aaggtgagcc acaacttgaa gccatgatag aagctagaaa
    13081 tttggatgag gtaagagttt ttagtagaaa caaggaaagt agagaaaaat ttgcagaaga
    13141 aatgaactta aaattaagta aatataatac taaaatagtt gcagttgaat catctgatga
    13201 agccatagat aacgcagatg tgatagtttt agctactcca tcaaaacaac cagtacttaa
    13261 tggaaactta gtgaaaaaag gagctttaat aagtgcagta ggttcatata tgccagatat
    13321 gcaagagttg tgcccaaact gtttaactag agcgtctaag atattttttg aatcaacaga
    13381 tgcagtttta tctgagtcag gagatatatt aattccatta aaagaaggta agataagtaa
    13441 agatgatttt agtggtgatt taggaaatgt tataaacgga actattcctg gaagagaaga
    13501 tgatgatgag ataattgtat ttaaaactgt tgggataggt gtacaagatg tcgtaacagc
    13561 aaaaagaata tacgataaag caaaagaaaa cggtattgga atagactgga agtggtaaaa
    13621 tattcaaaat aattctatag ttagacaaat ttatgttata atgttgataa ataaattttt
    13681 tattatttta attgagggag gatgtttgta tgaaaatggt aaaaaagtta atattatcta
    13741 cgattatggc tctagtttta gttctaacta tagggtgttc taatgcagaa gaaaaagtaa
    13801 agtcgaattt tgaagctaat ttagctgcat tagaaatgta taagtatgat gaaaaaggaa
    13861 tttctgatgc attaacagaa gctgaatata aacagattca aacttataac agcaaggaca
    13921 aggtttcaat gattgctcaa ttgacacctc tttatggtaa agaaaatgct gaaaagatat
    13981 acgaaacaat tgttaaatta attcaaggtg ttgaaaagac agtaacagtg gaatcttcta
    14041 gtaaggataa gataactttg aaagttgtaa gtaaagcttt agatactagt aaagtaatga
    14101 gtgaagcgaa cactttagta aaatcttatg taactgatga aaagaattac gataaaaaag
    14161 aaaaagaagt tcaagatgat atgacaaaaa tgattataga cctttacaat aagaaaccaa
    14221 ctaaggaagt tacaactatg attacatata ctaaaaaaga tggtaaatgg caaccaccta
    14281 gtaatataca aaagttatgt gaaggaatat atacaattta atttatttta aagacacaaa
    14341 actatctttt aagcatataa taaaatgttt ggaagatagt ttattttatt ttcaaagtat
    14401 aaattatatt ttataaatga t
//
LOCUS       DAEOSP010000095        14316 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.095, whole genome shotgun sequence.
ACCESSION   DAEOSP010000095 DAEOSP010000000
VERSION     DAEOSP010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14316
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.095"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            97..918
                     /locus_tag="KRH54_002180"
     CDS             97..918
                     /locus_tag="KRH54_002180"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBG8579837.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYNRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(994..2382)
                     /locus_tag="KRH54_002181"
     CDS             complement(994..2382)
                     /locus_tag="KRH54_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBG8579838.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVHNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGTLCIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(2517..3275)
                     /locus_tag="KRH54_002182"
     CDS             complement(2517..3275)
                     /locus_tag="KRH54_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBG8579839.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSSVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLICGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(3277..3993)
                     /locus_tag="KRH54_002183"
     CDS             complement(3277..3993)
                     /locus_tag="KRH54_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBG8579840.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIASILIILLFMWQIPLCMFLASKFGMFVTVLTNMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(3995..4702)
                     /locus_tag="KRH54_002184"
     CDS             complement(3995..4702)
                     /locus_tag="KRH54_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBG8579841.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGQ"
     gene            complement(4818..5354)
                     /locus_tag="KRH54_002185"
     CDS             complement(4818..5354)
                     /locus_tag="KRH54_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG8579842.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(5456..6526)
                     /locus_tag="KRH54_002186"
     CDS             complement(5456..6526)
                     /locus_tag="KRH54_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBG8579843.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAELNK
                     ENKLYQEKVNIDKLDIAVDNINVMIRKSSNEDVRVTTRGNNEFYKYSVTLQDKTLVVK
                     GERKSENKIKKIRSFDHLLNTSINSMFSRDYREIRIYVPNNVDINASSISSYLFISDD
                     VDSNNITYKTSSGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENGDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHNEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(6519..7577)
                     /locus_tag="KRH54_002187"
     CDS             complement(6519..7577)
                     /locus_tag="KRH54_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBG8579844.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINENSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(7564..7896)
                     /locus_tag="KRH54_002188"
     CDS             complement(7564..7896)
                     /locus_tag="KRH54_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG8579845.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            8095..9216
                     /locus_tag="KRH54_002189"
     CDS             8095..9216
                     /locus_tag="KRH54_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBG8579846.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(9344..11242)
                     /locus_tag="KRH54_002190"
     CDS             complement(9344..11242)
                     /locus_tag="KRH54_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBG8579847.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            11557..12333
                     /locus_tag="KRH54_002191"
     CDS             11557..12333
                     /locus_tag="KRH54_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG8579848.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKSSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMWKTNQFTLENVKQRKNK"
     gene            complement(12425..13177)
                     /gene="xth"
                     /locus_tag="KRH54_002192"
     CDS             complement(12425..13177)
                     /gene="xth"
                     /locus_tag="KRH54_002192"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBG8579849.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(13338..14288)
                     /gene="galT"
                     /locus_tag="KRH54_002193"
     CDS             complement(13338..14288)
                     /gene="galT"
                     /locus_tag="KRH54_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBG8579850.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
BASE COUNT         4843 a         2375 c         1547 g         5551 t
ORIGIN      
        1 tttataatct acttttttct tttttttata gtaatatata tgtatataaa tattttggta
       61 tcattataat taatacatac taatggaggg ttttttatgc aaaaaaaagt cgcagcaatt
      121 aatgacttat ctggaatagg aaaatgctca ttatcggttg ctatacctat actttcagca
      181 ttaaaggttc aatgttgtcc atttccaacg gccatacttt caagtcaaac tggttatcct
      241 gaatttactt tcttagactt tactgatgaa atggtaaaat attcaaatgt ttggaaaaat
      301 ttaaaagtca attttgatag tatatatagt ggttttttgg gttcaaagca tcaaattgag
      361 atagtggcta attttataaa cgattatccc aatgctttta tcgtagttga ccctgtgatg
      421 ggtgataatg gagttatgta tccaatattc acagaagaaa tgagacagga aattaaagaa
      481 ttagttaaac attcagattt aactactcct aatttaacag aagcttgttt cttaactgga
      541 aatgattata ccaaaagcga ttataataga gatgagctta tatatattgc aaaatctgta
      601 tctgatttgg gacctagcaa agtcgttatt actggtatat tagaagatga taatatatta
      661 aatttggctt acaatagaga taatgaccat gtattcttta catcagtcaa atataataat
      721 tgttcttaca gtggaactgg agatatattc acatctatac tttgtggtat gttagtaaat
      781 aaacatgatt tgggagtagc ggttaatact gcaacagatt ttatatataa aactataaat
      841 tacacatctc aatttgatac tgatagaaat gatggagtta tgtttgaaaa ctttttatca
      901 gatttaacca atatataaaa tcaaaaaaac tatccatttt ataaatacct ataaatcata
      961 aaatttaggt atttacaaaa tggatattat ttactaaact attggtattt caattgttac
     1021 catagcacca ccagttttca cagaattttc aatacataaa gtcccattat gtttttgtac
     1081 aatattattt acaatataga gtcccattcc ataatgagat ttagaatttc tactcaaatc
     1141 tcctgtataa aactgttctg tagcttttga caaatctgct ttagaaaaac cacttccaca
     1201 atctgtaact acaaatttta agtatttatt atgtactgat acacttatgt atagtttact
     1261 ctggcttggt gaataatcta tagcatttga aattatattc ataatagctc tgaataacag
     1321 ttctctatct atagttatat ttttaggcaa attatcttgt ttataaaact ccacatctaa
     1381 gtttttagta ctagctaatg cttctatttg cactaaaaca tcttttataa actcactcat
     1441 actgattgct tctttattta atatatagcc attctctgtt tttgatatat ctattaaagt
     1501 attaatatat ttttctattt gagtgacacc ctttgagata tattccatgt attctaataa
     1561 ctcaggattt tcatttattt caattaataa gtcagtattt ccatgtataa ttgttagtgg
     1621 tgtttttata tcatgtgcca gtgcagatat ctgttccttt ctattttctt ctagcatcca
     1681 ttgtttttct aaagaatctt ttaacgccaa cttcatttta tccatagaaa aaaggacatt
     1741 attaatttca taaattttac tagattctac aacaaaatct aaatcctgtt gttctatttt
     1801 ttctgtagta ttttttaaaa gctccatttc aatattaaat ttcttgccaa atacttttga
     1861 taatataaca atttcaataa aaaatattat actaaaaata atcataccca atgtctctgg
     1921 ttttggtaaa tatgctctta aaattggaga actatactct gatactattt tgtattttat
     1981 tataaaaact tcatctttct taaaaaactt tatataactt tctgaatatg catgcctttc
     2041 ttcaatatct ctcattaaat tccatacttc ttttgattct tttttattaa aggtccctga
     2101 aattaaattc ccatccaaag tataaacccc atactcatat atacttggaa ttatatcttc
     2161 agttactttt tcacttgaaa taatctgttc tttatattta ttaaattgtt tttctgcata
     2221 attagctggt aaaattacac ctgatgataa taatacagaa aatgataaca ttaaaaggag
     2281 tacaattgaa attgtcataa taaagaataa agataaatat tttataaaaa tagttcttaa
     2341 cttttcaact tttttgttgt tagatttttt acctccaagc atttgtaccc tgtcctccaa
     2401 tagtttaatt aattcataat taatacccaa ccagtttttt atttgattat tttatatctt
     2461 tttattgcta agttatattt taacataata atataaataa acagtcaatt tttactctat
     2521 tcttctgatt ttttaccttc ccatttagaa aaccaaatac ataaaattac aaatgctaaa
     2581 attgttacaa aaccacaaat taaacctgtt gattgatatg cttttattga ttcaaccttt
     2641 atatattcaa ccgttttaga acttgaaaaa gacgaccaaa tagttataaa tctaacaccc
     2701 catgcacaag gaaaatactg ccaaatacca gaacccaatc cagttaataa taatgctgaa
     2761 agtaacattt caactatacc tactccaatt gatgttcctt ttccgaacct taaacttaag
     2821 aataaatgta atatgtattc aaataaatta cacccaatta aaattagaat tgaaatcata
     2881 taaaagcttg atgtaaagta agccttagat ataccaaatt taaaacctaa tacagacact
     2941 aatgttgcta caaaaccaag acatactaag attatatatt tacttataag agtaagatat
     3001 tttggattag atgttgttaa catatgttta taattccctg cataatattc ttgttcaaca
     3061 aacattgaac acacaacact tgacaataaa ggaaaagcta ttgctataac ttgtaaatat
     3121 gctactatct ttagtatatt atcagctcct gaaaccttat aataccataa aaacaatccc
     3181 atccccatta aagcaatgca aatgtgcatt agtattataa aatttctctt taattttatt
     3241 aaatcagaac aaaatattct tattaactgt ctcatattac ttcacctctc tattattgaa
     3301 ccaaaccctt gtagctaaag caaataatac aaataaaaca atagatatta atactcctgg
     3361 caaaataaca gcattattta aaaattcact tccctctggt acaggaagac catttggcag
     3421 aatatgcaat acaacagcca ttaatcttga aggaatcgaa tatggaaaca tataccacat
     3481 ttctgtagtt gagaaaacag caactccaac aatagtaaag aacatatttg ttagaactgt
     3541 aacaaacata ccaaattttg aagctaaaaa catacaaaga ggaatctgcc acataaatag
     3601 taaaataatt aatatacttg caatgatatt ttgacctatt gaaatagttt ctttatagat
     3661 tatcccaaca aaaactacac ctattgcaga aataattgat gctataaaac aacaaataat
     3721 acagtaaatc atttttccag tccaaacgtc tttcatttca attggcaatg gtaaaatacc
     3781 acgatattta cttttttcat cttttaatac tattaaagaa gatattaatg ttaatgctcc
     3841 tggaagaaat gtcgtatacc accagttgaa agcagcattt tgccctccac ctagtccaca
     3901 caaaagtata caaaacactg gaactatcca tatcaacttc attccaaaag ttcctttcat
     3961 cttttgaaat tccactctaa taaatctttc catattactg ccctctcttt ctattttcag
     4021 ctacaacttt cataaaaagt tcttctaagt tttgttcttt tgatacttct ccttgataac
     4081 ccattactcc attagaaatt attcctattt catctacaac ttgttcaact tcagacaaga
     4141 tatggcttga taaaataact gtaattcctt gttttggaaa agaacgaatc aattctctaa
     4201 gttcttcaat accaataggg tcaagaccat ttgtaggctc atctaagatt aaaagttttg
     4261 ggtgatttaa taatgcaata gcaataccaa ggcgttgctt catgcctaga gagaattgac
     4321 ctgaacgctt tttaaaggta tctcttaaat ctacagtctc taaaacttct tcaatgcgag
     4381 agtcaggaag accaagtaaa gtagtatgga cttttaaatt ttctcgtgct gttaaattct
     4441 catatatagc aggagactca ataagagaac caatatttga taaatccttt ctagtccatc
     4501 tatgtccctc aaacattatt tttcctgaag ttggatttaa aatcccagtt atcattttta
     4561 ataaagtaga tttcccagct ccatttggtc ctaataatcc atatatagaa ttagatttaa
     4621 ttgctagtga aatgttatca actgccatct gctccttaaa ttttttagaa actcctttta
     4681 cctgcaaaat ataattactc ataattttca tcctttcttt tgtcaaactt atttacaaga
     4741 atcattctaa atcataaata taaggatttt ataagaatta aaaaacaaaa aaagatgtgt
     4801 taaaaacaca tcttttttta tcttaattct attaattttc cattgtagta tatctctact
     4861 tcttcaatcc aaatgctatt atcttcttct tggatgacat ttttaataat attatttata
     4921 gtatatatta tattttgttc attcaaataa tcatctttcc aagaaactct acttttttta
     4981 tctggttcta tcaagtttat tttaacaaga ttatcttttt ccttaacttc tacttccatg
     5041 tctaaaccat taaaatactc atttgataat gtatctaaga ttaatcgcag tttatcctta
     5101 agtgaacttt cttcatcaat ctgtaagtac tttactattt tctcatcctt tgaaattatc
     5161 tctataggta ttttagtagt ttttttagaa ttaatcattt tactagttgt ttcttctttt
     5221 gcatcttcat ccttctttac aactgctgat tctttagaat caactagctc aactgcactg
     5281 atgctataat ccatacatgc cgtactcaat aaagtagtag caattattaa aaaacttact
     5341 gctattttat tcataagtat ccctctccta attctctttc gatacttatt ataccatagt
     5401 atagattata tttttatact tttaaatcaa tttataaatt attatagtaa attatttaca
     5461 attctaagct atttgaattt atttttatag tatattcttt ctctaaattc gacagatttt
     5521 tattcaaaag cccttttatt tccctcgtat ttctatactt gttgctatgt tcttcattat
     5581 gttcataatc atatgaatca ttgtcatatt cttcatcatt attaaattct tcaattgctt
     5641 cttttgactt tatatcacaa ttaaatttat acttatttac tggtatatca agtcttacct
     5701 tagaatttgg aacttctatg tccaggaact tagcaattgg catatcagag tacaagtaaa
     5761 attcactttc ataatttgaa tttctaccta ttttctcttt tatatttaca ttttcaggta
     5821 tatactcttc tatattattg ataaatacat ctccattctc tgaagatata tccaatgact
     5881 ctgattctat gtttacattt ttaacgccta aaattgactt tgttgataga tgaagattgc
     5941 tattagatat taagtttaag ttttctaatc tatttacttt attttctgtt atagctctgc
     6001 taaaacctcc agaagaagtt ttatatgtta tattatttga atctacatca tcagatataa
     6061 ataagtacga acttatactt gatgcattta tatctacatt atttggtaca tatattctaa
     6121 tttctctata atcacgtgaa aacatactat ttatagatgt atttaataag tggtcgaaac
     6181 ttctaatctt tttaatttta ttttcagatt ttctttcgcc ttttacaact aaagtcttat
     6241 cttgtagggt tacactgtat ttgtaaaatt cattattccc tcttgttgtc acacgcacat
     6301 cttcatttga actttttctt atcataacat ttatattgtc aacagctata tctaacttgt
     6361 caatatttac tttttcttga taaagtttgt tttctttgtt taattcagct tccttttcta
     6421 aaccaaagtt tattaggctt ggcataacta aaatccctga ccatgttgtc cctattatac
     6481 ctattaccaa taaaactgca gcaaatatag caagtttttt attcatgttt atcttctcct
     6541 ttcttatttt ctatatcttc aaggtcctta tatatcgcct caaaatttat actatcatca
     6601 ttatattcat cagtttcttt tatgctttct tcttctctat tgtagtaatc attattatat
     6661 gaattttcat taatgtcttt attcaaatct tcatcattta aagtagcttt ttttcttttt
     6721 tccatctttt catttgcctt tatataaata ttttttctct ttatccaatg tatataatta
     6781 tttagtaatt gtttacctag ttttatggca aataataaaa tttgccatgc aagtatctca
     6841 agccctacaa atgctatggt tgcgaaaata caaaacatca taacatctgg aaccattgct
     6901 ataaatttaa tcacaaatgg aattaatata acaaaagcaa ttaatgagca tactaatgca
     6961 atagtagcag atacaatagc acatacaaac gcaaatgctg gaacaattag aaataaagat
     7021 aacactacta ataacccctt tataacttgt ctggataatc ctcctacacc ttcttctata
     7081 cttggtgtaa gtttatcatt tatattttct tttgatttat tgtacttttc cttagcttca
     7141 tctttcaaga tagaataatc cttttttaca tttgatttaa atctactaaa tttaccattt
     7201 aatatgtctg tattcattat ttttccatct atattttctt ttgatttact atacttttct
     7261 tttgcttcat cttttaagat agagtaacct tttttcatat ctgatttaaa tttactaaac
     7321 ttatcattca aaacatctgt atttattatt ttttcctctt caatattttt ttctttagtt
     7381 tgtaagataa gttcttttgc tatagatttt ggtgagccaa gtgatgctat tatctccata
     7441 tcactctttc cctctataag accatctacg aaatactctt cataatctct caatatatca
     7501 ttaacttcgc tctcagaaaa atcttttttt agataatcct ttaaaatttc aagaaactca
     7561 gttttattca agttcattac ctccctcatc ttcacttatt aaaatctcaa cagcctttac
     7621 aaattctctc cactcttcca tcagattgga taaattctct tttcctagtg aagttattct
     7681 atagtatttt ctagcaggac cttcattaga ttctagtata tatgtttcga aatacaaatc
     7741 ttttgtaagt cttcttagaa taggatatat tgttccttca tttacatcta ttactttaga
     7801 gatgttatgt actatctcat atccatacat atctttctta gatatgagag caagtacaca
     7861 aatttctaaa acaccctttc taaattgtgt gttcatatac tccatccttt ctttaaatac
     7921 acagtattgt gttttacaag gtactatgtt aaaacttctt atatcctcat aataacacag
     7981 tactatgttc tgcacaatag ttatttttaa atattcttgt tgattaaatc cttctataaa
     8041 tgtataatac tctaatcaga tatattttat gaaagtggtg ataaattggc taaaatgaaa
     8101 gatgataaaa caatattaat cctaactgct caatttggtg ctggacatat aagtgctgca
     8161 aaagcagtca aagaatgtat tattgaaaaa tatagtaact ataatgttgt tatacaaaac
     8221 tttataaatg caagtattcc catgatgaat aagcctatgg taaaacttta tgaaaacaat
     8281 acaaaatata ctcctggatt atataattac tattattact ttaagaaatc gtttgattca
     8341 agacatgatt tatctcataa attatataca cctaaacttt ctgagtatat tgctgatata
     8401 aatccagatt tgattatctc tacatttcca ctagctgctg cttgtgtaaa taactttaag
     8461 ataaaaaatc ctgatataaa tatacctaca cttacagtta taacagatgt tgtagatagt
     8521 atggaatggg tttttgaaaa tacagatttg tattttgttc cttctcctga aataaaaaat
     8581 agattttttc agaaaggaat aaatccagac tccataaagg ttactggagt tcctgttgat
     8641 aagagatttc aaattgaaag taaagaacta tgttgtgata aatatagact attacttcta
     8701 ggtggaggaa gaggtctatt tgatatagat gaggatttta tgcattggat agatgaattt
     8761 attgaagaac atagtaattc tatagaaatt acaatagtta ctggtaaaaa caaaaaatta
     8821 tatgacaatc taactcataa aaaacctcta aaaaatatta aagtacttgg atttgtaaac
     8881 gacatgtata atctaataag agaatgtgac ctaatgctaa ctaagcctgg aggagcaact
     8941 atatttgaag ctattcaatc acaaactcca gtactcgtaa aaatgccaaa agttggacaa
     9001 gaaattgaaa acgctaaatt tataatagat aagggtcttg gcatgattta tagtgatgac
     9061 ctagacctta aaaacatatt ttataaatta gtatcaaatg aatttgattc tataataaac
     9121 tttatgaaaa aaaatttaaa agaatttaaa acagtcattc atccagagaa aattgctgat
     9181 tatatatcag aattaataga taatcattac agttaaattg tactattaaa aaatttcaaa
     9241 cataaaatta aaggcaatca agtatgcttt tacacttgag aatttactca tagtgtttaa
     9301 taatacttga ttgcctttat catatcaaaa tcaaattgtt atattagtat atatttttct
     9361 tgtactgcat atacagttgt tgtatttctt ccatacttct ctttaattct atttgtaaat
     9421 ctgaagcagt ctggtaatcc ttattttcta tagcttctct cactctagtg aatagtgatt
     9481 ctctagttaa agtatccttc ataatattga actttaaatc cgatattgag ttccaaacta
     9541 cctcatcaag cgcatccata ttttcttttg tgtgtatctt gacaatactt cttatttttt
     9601 gaatcccagc ctctattatt ctagttttta atttcttttg ccacttatct atagctccaa
     9661 tcttaaatga ttctattata ctatcagtaa atacatcacc ttgttttaaa gattttaatt
     9721 ttgaagcata tatttctaag tttttcatat tttcataaac tgtagccggt ggtattccaa
     9781 atcttgcatt tctttcttcc aaactataat gttcaaatac atcattttca tctctatatg
     9841 ctctatcttt ttctaaatag aaactttctt cacctacatt tttagataac tctttttcaa
     9901 gttcttttgt actaagacca ctcttagctg ctgcttttat accatccaac attgtttgat
     9961 aacatcctgc tattactaaa tacgtgttag atagagggct tggtgacctt aactcaaatc
    10021 ttacagtctt tggatttttc atatctctta ttaatccaac aagcactgac ctatttcttg
    10081 atggaatctc ataactatga cctaaagatg taactatgca tacaggagct tcaaatccag
    10141 gaactaatct attaaatcca tcattagaag ctgttacaat tgggtttaat acttcataat
    10201 tgtaaagaag tcccataaga gccccatatc ctagttcact tagataatct tcttttaaat
    10261 cttttggcgc aaatagattt tttatacttc catcctttaa cttcgcactt actcctacat
    10321 gtgtatgtcc accacttcct gctactccat gaattggttt tgctttaaat gtaacttcta
    10381 gtccatgtga tgtaaataca tcctctataa catctcttac taataattca ttgtctgctg
    10441 cttgtaacgg tgtagaaaac ttccaagaaa cctctaattg ctccatagca tggtttgttc
    10501 ttccatctat actaatagaa ctttgtattc ctccgacctc tttgtgagcc atttcagggt
    10561 ttacacctag cttttgaagt ataattaaac ttttctctaa acaagttctt ataataccat
    10621 gagttctttt ccagtattgc tcttttaggc tttgtgatac atataatttc tctaagtcag
    10681 ctttatcctc aggagtatta acccagaact caagttctgt agctgctgtt aacattattt
    10741 cctctatatc atctactgaa tctattccta tgttatttat tacatgtgga tattctctaa
    10801 atatttcata cattgacttt ttaaagtact tatctgcttt ttgaagtact cctctagaac
    10861 atactttttt attatcatgt attaaaaatg caggtatctt taatgttcca acaggtagac
    10921 ctacttcttc gtctatatat tccatattgt aatctacata ccaatgacaa cttttatctg
    10981 gcattaaatc tactttcgca ttatttaatg tagctatatt ataaagctca acacttgaac
    11041 catcagtctg tatagctgat tccaaaaatt tatctatatc ctccaaaaat aattctacag
    11101 gaatcttttc atctgttgca tttcctccta aatcaacccc cattaaggaa acaaatctta
    11161 tatttttatt gctttttagg atttctctta attcttcatt agtgtgttta tctttctcta
    11221 ttacgtataa tagactatcc atttttccac tctcccataa tttttatgta tattttattt
    11281 tgttaaatac aagataaatt tcattttcct tacatttatg aatttttctt gcaaattatt
    11341 cgtgttttat tcgtgttttt atttatatta ttatatatca agtataaaaa gttcgcaata
    11401 ttttttattg gaaaaaatat tatacataca atagtttaac atttttacaa atatggggta
    11461 tactaaaata aaagtatttt tatttgaaaa tttaatttta tttataatat aatctatagt
    11521 aaaagaaata ataaaaaaat ttaggagata aactttatgg aatacataat tttcgatttg
    11581 gaatttaacc aaggatttga taaaaaactc aataaaactg tatccaatga aaagtgtcct
    11641 tttgaaatta tacaaatagg agcaattaaa ttggattcta aattcaatat aatagatacg
    11701 tttaatagct atgtaaaacc tacaatttat aaagacatac atccctttat tagtcgtatg
    11761 actaatatta aaagttctga ttttaatgat tcgcctactt tccctgaggt ctacaacaat
    11821 tttataaaat ttatatcatc acaagaccct atattatgtg tttggggagc tggagatttg
    11881 aaggaattat acaggaatat caactatcac aagctaccat caaattcctt acctaaatct
    11941 tatattaata tacaacaaca tgcatctaaa tatttcaata atccagcagg aaaaagcatt
    12001 ggcctacaaa atgctatttc tatattggaa cttgatgaaa aaatgtctta tcacaatgct
    12061 ttaaatgatg catattatac tgctaaagtt tttattaaga tatacaatcc aagtatagtc
    12121 ccagatatat atctttatac gagtataaaa cctaaaacta taagatattc taacaaaaaa
    12181 agagttgatt atgataagtt atttgatgaa tttaggaaaa ttttaaatcg tgaattaact
    12241 aaagacgaga aaaaaatcat taacttagct tataatatgt ggaaaacaaa tcaatttaca
    12301 ttagaaaatg ttaagcaaag aaaaaacaaa tagagtttat ttagataaaa aataggcatg
    12361 tagattaaaa aactagttaa ctttaaattt atatctacag cccaattttt atcataatta
    12421 atttttattt tatctctaat tcaactggac aatggtctga acctaatatt tcagtgtgta
    12481 tatccgcact tactaatcta tcttctaatt ttttggatgc acaaaaataa tctattctcc
    12541 accctgcatt attttttctt gcattaaatc tatacgacca ccaactataa acaccttctt
    12601 tatcaggatt aaaatatcta tatgtgtcta taaacccact gtctaataat ttagaaaact
    12661 tctctctttc ttctttagta aaacctgcat ttttcatatt atttttagga ttttttaaat
    12721 ctatttcttt atgtgcaacg ttcatatctc cacacacaat tacaggctta tgattatcta
    12781 gtttggttaa ataatctata aagtcgtctt cccatctagt tctatattct agtctcttta
    12841 attcagattg agaatttggc gtatatactg taacaaagta aaaatcttca aactctagag
    12901 ttatcactct accctctttg tcatgttctt ctatatttat tccatacatt acttttaaag
    12961 gctctttttt tgtaaatata gcagtacctg aataaccctt tctctcagca taattccaat
    13021 attgaaaata tcctggtaaa tctaactcta tttgcccttc ttgcaattta gtttcttgta
    13081 aacaaaatat atctgcatct acttctttga aaaagtctaa aaatcctttc ccaacacaag
    13141 ctcttattcc attaacattc catgatataa atttcatact tatactcctc atctaaacat
    13201 tgacacactc cccatagcta aagatggggg cttttcaaca attttattgt aaattcttaa
    13261 tttactttta ttttaacaat gctatactat aatttcatct aaaattacta tttacttaat
    13321 acctaaaaat atatgtttta atcaattttt aacttttttg ttaaatcttc agggctcatt
    13381 gaagaaacat acatatttgt tccaagttca aatcctccaa aattatattt tcttgggatt
    13441 atgtgcatat gaacattaaa atacgatttt gtttcaatct ttgttggatg agtgtgaata
    13501 cataaattaa atggcatata tccattgata ttgtataact tcttaaacaa cttttcaaat
    13561 attgtagaaa gctcctgtat attattatca tttaactcac caaactctat tttatcttta
    13621 aatattattc ttacttctcc actatacttg gttgcatatg gtataagtac caaaaaagct
    13681 tctccattat aaacaactct tctatctaaa cttatttcat tttctattaa atcatcatac
    13741 aaagagcttt tattgctgtc ataatactcc tttgaaacca acagttcatt cgtaatttca
    13801 ggtggaataa aagacataga caatatttga gcatgtggat gcatcaaaga tgctcctgcc
    13861 tttctcaaga agtttttgaa gatactaaca tactctactt tatcatcttt cgacaaatct
    13921 ctaaatctat ttctatacat aaaaaaaaca tcttcaaatt ctttttgact catgttataa
    13981 aaactacgat tatgtttatc gctctctatc attacttcat gaacaccata tacatctctt
    14041 gccatatcat caattatagg aaatttattg tagacagatt ttacaagcca tccttcttca
    14101 ctatctattt caaataatgc atcagttgca tgagcttcat ttcctttgca aaatgggcat
    14161 tcttcatcat attcattagt ttcttcatct tcatttggga tatctgctgt atctaaaggt
    14221 ctttttaacc tatctgtagc aaatataaca acatcattgg taattggatc tattcttaat
    14281 tctttcataa ttaacacctc ttagttaata gccttt
//
LOCUS       DAEOSP010000096        14315 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.096, whole genome shotgun sequence.
ACCESSION   DAEOSP010000096 DAEOSP010000000
VERSION     DAEOSP010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.096"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..835)
                     /locus_tag="KRH54_002194"
     CDS             complement(<1..835)
                     /locus_tag="KRH54_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG8579851.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MK"
     gene            1252..2655
                     /locus_tag="KRH54_002195"
     CDS             1252..2655
                     /locus_tag="KRH54_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579852.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            2682..4226
                     /locus_tag="KRH54_002196"
     CDS             2682..4226
                     /locus_tag="KRH54_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG8579853.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            4257..5648
                     /locus_tag="KRH54_002197"
     CDS             4257..5648
                     /locus_tag="KRH54_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBG8579854.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            5841..7337
                     /gene="abfD"
                     /locus_tag="KRH54_002198"
     CDS             5841..7337
                     /gene="abfD"
                     /locus_tag="KRH54_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBG8579855.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            7472..7747
                     /locus_tag="KRH54_002199"
     CDS             7472..7747
                     /locus_tag="KRH54_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579856.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            7771..9078
                     /locus_tag="KRH54_002200"
     CDS             7771..9078
                     /locus_tag="KRH54_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBG8579857.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            9098..10216
                     /locus_tag="KRH54_002201"
     CDS             9098..10216
                     /locus_tag="KRH54_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBG8579858.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            10503..11423
                     /locus_tag="KRH54_002202"
     CDS             10503..11423
                     /locus_tag="KRH54_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBG8579859.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            11538..12695
                     /locus_tag="KRH54_002203"
     CDS             11538..12695
                     /locus_tag="KRH54_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBG8579860.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(12780..14279)
                     /locus_tag="KRH54_002204"
     CDS             complement(12780..14279)
                     /locus_tag="KRH54_002204"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBG8579861.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT         5490 a         1735 c         2646 g         4444 t
ORIGIN      
        1 gtttcatact atgagatatg tattgatttc ttctccaaag aaccccaata ttcgcagtaa
       61 ttgcacgttc taatggtatt gaacagagat tttctgtatt ttgaaatatt tctttataca
      121 atattgttga agccaagtca ttttctagta aataacgtat ggtagaaagt tgatttgagt
      181 gtaaaattat atttggctta gctttttcaa gtctttttgt aataatctta tagtgataag
      241 aaccttcaga taagattaca aaaggtacat tttttaacat ataattcgat ataatgggat
      301 gttttgccaa ttcatttttg ggatttgtaa ctaagtataa ttctgtttta tagattgtct
      361 ttgaatctat atcatcataa atatcactgt caattatacc aatacaaaaa tcaagtgtag
      421 attcttcaat cattttagct ccaacaaatg ttggaacttc aaaaatttta agctctatat
      481 ctggataaat tacattaaat ctaggaagta tttttgaaag caaaaacgta cctaaaactg
      541 taggaatacc tattttgaga gaagcttttc tcttttttcc taaatcaagt gcgtcacatt
      601 gaaaatcctc aaattgtttt ataaaatgtt ttgacttgtc taaaaaaatt tttccttcag
      661 gcgttaactc tattttatta cctattctgt aaaaaagttc aaagccaagt tctttttcta
      721 attctttaat tgctaatgtt atggatggtt gagaaatatg aatactttcc gcagccttag
      781 tcatgttttt atatttacag acagtttgaa aatagtataa atagtttata ttcatttggt
      841 attagttcct ttcatttaca tattagtgac aagtatataa ttaacaatag cataagtttt
      901 gtatattgtt gtaaaaaaat agaatagttt taaactatca tagcataagt ttttgatatt
      961 ttcaatctat taagaatagt gctattatta aattaacaaa agagaatagc tggtatggtt
     1021 tagagtaaat caatctatat tacagttata tcaacgaata tcaaaaaatt atctattaga
     1081 aaataaaaat tatttttaca attaaattgt taattaaatc ataaagaaaa aagtgatatt
     1141 aacaagataa aattttttta atcagaaatg ttaatttaat aacaaaatag agtattgggg
     1201 agaaaggaga aatatatttt ttaataatat ttgttaattt aataacgaaa aatgaaaggg
     1261 agtgatttag tgaagtttga attgatggat tttttaatga atccttttgt ccttatgttt
     1321 gctgcagtaa ttacaggaat tttatttgga aaaattaaat ttgggaagtt taattttggg
     1381 gtatcaggag cattatttac aggattattt attggatggc ttgcatatag cttggggaat
     1441 ctaataatag caaaagggga aacagcagca ggatataagg cagcaactgt aatgatgggt
     1501 aatggaatta tttcttctga cttctttgat ttctttttaa taatatttgt tgcagcagtt
     1561 ggtctattgg cagcaaaaga tatgaaagct gtattaaaga agtatggagc aagatttgta
     1621 attttaggag ttcttataac ttttataggt ggctctatga cttatgcaat gactttacta
     1681 tcaagtgata aaggaagtag tgcttatgaa gtttctggcg tttacacagg agcattaaca
     1741 agttcgcctg gattagctgc tgctttagaa acagctggta aacatgcaga agatgtatca
     1801 aaagaatttg aaaaagcttc aataaaagat aaaaaagaaa ttttaaaagt agtcgaccca
     1861 gaaggcaaat tggatgtgaa tacaactaca tcactgactc aagagcaaat agataagtat
     1921 attgcatatg ctgaagctgg tgttggtata ggtcatgcag tcgcatatcc ttttggagtt
     1981 ttaatagtaa tcttaggtgt aaatttcctt ccaaaattat ttaggatgga cctaaaagaa
     2041 gaaagaagaa aatatgagaa ggaaatgaag gaagcaagag atagtgtttc tggtaaaacg
     2101 ataccagaag ttccatttaa tataataaca tttttcttga catgtttagc tggatactta
     2161 gttggaggaa tacatgtatt tatgggacca ctagggtact tcacactagg tgcaacagga
     2221 ggttcactaa ttgtatctct agttcttgga tatataggta agataggggt agtaaatttc
     2281 agaatggaag aaaaggtttt aaatatttta aaacaaatag ggctagtatt tttcttagca
     2341 atagttggat taagatatgg tggaaaagta gttgattcaa ttatgacatc tggtatgcac
     2401 ctagccttag tagcaatagc agtaggtgtt acagctatga tgataggatt cttagttggc
     2461 aaatacgtat ttaaattaaa ttggatttta ttatctggag ctgtctgtgg tggtatgaca
     2521 tcaacaccag gtcttggagc tgctgttgat gctcttgata gtgatgaccc agcagcaggt
     2581 tatggtgcta cgtatccatt tgcattatta actaaagtta tattggttat agttttacac
     2641 aaacttccaa tgtaaataag attttatatg gggggatatt tatgaataat gaaggagcat
     2701 ttgaagaatt aagtggaaga ttaagaaata aagaattgtg tgatttaata atgaatgcag
     2761 atgaagctgc taaaattata gaggatggca tggttattgg agttagtgga tttacacctt
     2821 caggctatcc aaaagcagta ccattagcag tttcagaaag agctaaaagt ggagaaaaga
     2881 taaaattaac cgtatattca ggagcatctt taggtccaga agttgatgga gcatggtcag
     2941 aagcaggtat aattgaaaga agacttcctt atcaaactaa ttcaatactt agaaataata
     3001 ttaataaagg tgttgttgat tatatagata tgcacctaag tcattctact caatttttaa
     3061 attatggaac tatacctaaa gtagatgtag ctatagtaga agcacttgca ataactgaag
     3121 aaggaaatat tataccaact agtgggatag gtaattctcc atcttttata aaaagtgcag
     3181 ataaagtaat agtagagata aatcttgcaa aaccaatgga aatggaaggt atggcagata
     3241 tatatattac ggaaaatcca ccaaatagaa aaccaataga aataaatcat cctaaggata
     3301 gaattggaac aacatatata ccttgtggtt tggataaaat tgcaggagta gtaattacca
     3361 atatgcaaga taagacccgt ccattgggag ttgtagatga agcttcgaaa aaaatatcaa
     3421 ataatattat tgctttttta agagaagaag taaaggcagg cagattatca aagaatttac
     3481 ttcctcttca atctggtgta ggaagtgtag caaatgcagt attatatgga ttatgtgaat
     3541 ctgaatttga aaatttaact tgttatacag aggttgtaca ggactctatg ttggatttaa
     3601 taagaatggg taaagttaca atggcatcta ctacatctgt aagtccatct ccagaaggat
     3661 taataaaatt tgaaaaagat attgattttt ttaaggataa gattatatta agacctcaag
     3721 aaattagtaa caatccagaa atagccagaa gaattggtgt aattgcaatg aacactgcaa
     3781 ttgaagttga tatttatgga aatgtaaatt ctactcacat aatggggtca aagatgatga
     3841 atggaattgg tggttctggt gattttgcta gaaatggagc aataactata tttagtactg
     3901 agtcaattgc aaaaaatggt gatatatcat caatagtacc tatggtatct cacgtagacc
     3961 acactgaaca tgatgtaatg gtaattgtaa cagaacaagg atatgctgat ttaagaggtc
     4021 ttgcacctag agaaagagct ataaaaataa tagaaaattg tgcccatcca gattataaag
     4081 aacaacttag ggattattta aatagagctt gtaaaagtgg agcaaaacaa acacctcaca
     4141 tattggacga agctttatca tggcatagta aatttatgag tacaggtaca atgaaaaagg
     4201 ctgaaacttt taaaagtgca ttataaaata tttaataaat tggaggtaga ttttaaatgg
     4261 aaaaagcagt ggaaaacttt gaagatttaa gtaaggaata cattaatggt tatattgaga
     4321 gagcaagaaa agctcaaaga gagtttgaat gttatactca agaacaagta gataaaatag
     4381 taaaaatagt tggaaaagta gtgtattata atgctgaata tttagctaaa cttgctgtag
     4441 aagagactgg aatgggagta tatgaagata aggtagctaa aaataaaagc aaagcaaaag
     4501 ttatatataa caatttaaaa gataaaaaat cagttggtat aattgatata gatagagaaa
     4561 ctggaataac aaaggtagct aaaccagttg gagtagtagc ggcaataact ccttgtacaa
     4621 atccaatcgt tacacctatg agcaatgcaa tgtttgcact taagggaaga aatgcaatta
     4681 ttatcacacc tcatcataaa gcaattggat gtagtacaaa aactgtagag atgattaatg
     4741 aagaattaga aaaaatagga gctccagaaa atttaataca aatacttgac caacaatcaa
     4801 gagaaaatac aagaaattta atttcatcag cagatgtagt aattgccact ggaggaatgg
     4861 gaatggtaaa agctgcgtac tcaagtggaa aaccagcact tggtgtaggg gcaggaaatg
     4921 ttcaatgtat catagataga gatgtagaca ttaaagaagc agtacctaaa atcatagcag
     4981 gaagaatttt tgataatggt atcatttgtt caggtgaaca gtcagtaata gtagcagaag
     5041 aaatgtttga taaaataatg gatgaattta aaaataataa aggttttata gttagagata
     5101 aagtacaaaa agaagctttc agaaatgcta tgtttgtaaa taaatctatg aataaagatg
     5161 cagtaggtca atcagttcat acaattgcaa aaattgcagg tgttgagata ccagaagata
     5221 caaaaataat agtaatagaa gctgatggac caggagaaga ggatattatt gcaaaagaaa
     5281 aaatgtgtcc agttatatct gcatacaagt ataaaagttt tgaagaaggt gtagctattg
     5341 caaaagcaaa tttaaatgta gaaggaaaag gacatagtgt atctatacat tcaaatacag
     5401 ttaaaaatat agaatatgca ggagaaaata tagaagtatc aagatttgtt attaatcaat
     5461 gttgtgctac tagtgctgga ggaagtttct ttaatggtct tgcacctact aatactctag
     5521 gttgtggttc ttggggaaat aacagcatct ctgaaaatct tgattataag catctaatta
     5581 atatttctag aattgcttat tatatgccag aaaatgaagt ccctacagat gaagaacttt
     5641 ggggataaaa tttagacaaa aaatattcat tctaaattaa agtaggaata tattttaatt
     5701 tagaatgaat ttaagacata aattaaattt aaattagttt attggactaa atttaaaata
     5761 ttaagagaca tatagaagaa ggaagtatta taatttaaaa taaataaaaa aatctataaa
     5821 agagaaggag tggttttatt atggcattaa tgacaggagc tcaatatatt gaaagtttga
     5881 gaaaattaaa tactaaggtt tatatgtttg gtgaagaggt taagaattgg gttgaccatc
     5941 ctatgattag accatctata aattgtgtag cagcaactta tgacttagct catgacccag
     6001 aatatgcaga tttaatgaca gttacttcaa atatcacagg tgaaaaaata aatagatttg
     6061 gtcatcttca tcaaagtgtt gatgatttga taaaaaaagt aaaaatgcaa agactatgtg
     6121 gtcaaaaaac agcttcttgt ttccaaagat gtgttggtat ggatgcgttt aatgcagtgt
     6181 attcaacaac atttgagtgt gataaagccc atggaactaa ttatcatgat aattttgtaa
     6241 aatacttaac ttatatacaa gaaaacgatt tagtagttga tggagctatg actgacccta
     6301 agggagatag aagtctatca cctagtgctc aacctgaccc agatatgttc cttcatatag
     6361 tagaaagaag agaagatggg ataattgtaa gaggagcaaa ggcacatcaa actggttcaa
     6421 taaattcaca tgagcacctt ataatgccta ctataagtat gactgaagca gataaagact
     6481 acgctgtatc atttgcagtt ccttcagatg ctgaaggagt gtttatgatt tatggaagac
     6541 aatcttgtga cactagaaaa ttagaagaag gtgcagatgt tgatttagga aataaggagt
     6601 ttggaggtca agaagcttta gtagtatttg ataatgtatt tgttccaaat gatagaattt
     6661 tcttatgtgg agaatgggat ttctctggta tgttagtaga gagatttgca ggataccata
     6721 gacaaagtta tggtggatgt aaagtaggag taggtgatgt aataatagga gcagcagctt
     6781 tagcagctga ttataatgga gcaaacaaag cttcacatat aaaagataaa ctaatagaaa
     6841 tgactcacct taatgaaagc ttatattgct gtggtatagc atgttcatca gaaggtcaca
     6901 aaacagaagc tggaaactat caaatagatt tacttcttgc aaatgtgtgt aaacaaaatg
     6961 taactagatt tccttatgaa atagtaagac ttgcagaaga tatagctgga gggcttatgg
     7021 ttactatgcc ttcagaaaaa gactttaaat cagaccttaa agttggaaca agtggaatga
     7081 ctattggcga agtatgtaac aagtatttta aagcttcttc agtggcttca acagaagaaa
     7141 gaatgagaat attaagattc ttagaaaata tatgccttgg ttcatctgct gttggttata
     7201 gaactgagtc aatgcatggg gcaggttcac cacaagctca aagaataatg atttcaagac
     7261 aaggaaacat aaatcaaaag aaagaattag ctaagaaaat tgcgggaata aaaaaagagg
     7321 aagctttaaa tttataaaaa gttgatatat aaagggaaat tatatattaa tgtgtattaa
     7381 ttaatgaaaa aatatgtagt ataagagata gttataaaat tatggtggtt tttagctaag
     7441 taaagaaatt tttaataagg agaatttaca catgacctgt ggagttagat tttgtggggg
     7501 ctgtaaccct agatacgaaa gagggaaggc tcttacaata atcaaagagc acttcaatgg
     7561 tagggtagat tttagtatag cacaagagaa cgtcgagtat gatttgctac ttataattgg
     7621 aggatgcgca actctatgcg catcctatca acaatataaa gtgaaaaatg aatgtgttaa
     7681 gatgtgggat tttagccata tagaaagagt tataaatgtg attgaatcag caatttgtga
     7741 aacttaaaaa agctaaatgg ggggatttca ttgagttggc aagaattgta tcaaagtaaa
     7801 ttatgtagtg ctacagaagc tgtaaaacag attaaaaacg gtgatactgt agtatttgca
     7861 cattgtgtag gtgaacctcc tgcactagta gaggctatga ttgaaaatgc tgaacaatat
     7921 aaagatgttg agattaaaca tatggtaagc ttgggtagtg gtggatatac tgctaaggga
     7981 atggaagctc attttagagt aaatccaatg tttgtaagtg gaaatgtaag aaaggcaatt
     8041 gaaaatggag atggtgattt tacacctgca ttcttccatg aagtaccaaa gttgcttaga
     8101 gaaaaaagat taaaatgtga tgttgtttta gcacaggtaa ctccaccaga tgaacatggg
     8161 tattgtagct taggaacatc agttgattat acttatgaag ccattaaaac tgctagaaca
     8221 gtaattgttc aagtgaatga ccagtttcct agaacctatg gtgaggtagt ccatgtatca
     8281 gagtttgact atatagtgga aaaatcacaa cctttatttg aactacaacc tgcaaagatt
     8341 ggagaagtag aagaagcaat tggtaaaaat tgtgcttcat taattgaaga tggttctact
     8401 ttacagctag ggattggtgg gataccagat gcagttatgt tatttttaac tgataaaaag
     8461 gatttaggga ttcattcaga aatgatttca gatgggactt tggcccttta tgaaaaaggt
     8521 gttataaatg gtaaatataa aaattttgat aaagaaaaaa tgacagttac cttcttaatg
     8581 ggtactaaaa aattatatga ctttgcaaat aataaccctg cagtagaggt aaaaccagta
     8641 gactatgtga atcatccagc aattataatg aaacaacata agatggtttc tataaattct
     8701 gctatacaag ttgatttaat gggacaggta gttgcagagg ctatgggact tagacaattt
     8761 agtggtgttg gaggtcaagt tgactttata cgtggagtgt ctatgggtga agatggaaag
     8821 gcaattatag ctatgccttc aataactacg aaaaaagatg gtacagtaat ttcaaaaatc
     8881 gtgtctattg tggatgaagg tgcacctata acgacatcaa gaaatgatgt tgactatatt
     8941 gtaacagaat acggtatagc agaattaaaa ggaaaatctc ttagagaaag agcgagaaat
     9001 ttaataaata tagctcatcc aagtgtaaga gaaagtctag cagtagaatt tgaaaagaga
     9061 tttaaggaga aatattaata atagaggggt gttaataatg gaagcactta gagtagttcc
     9121 aaagatattt tactttgata catttaaaga atttaatgaa gaatttaaaa taggaaaaaa
     9181 tgatttagta attactaatg agtttatata tgagccatat atgaaacctc ttggaataga
     9241 tacaaattta atttttcaag aaaagtttgg aacaggtgaa ccatctgatg aaatgataga
     9301 ctccatgaca aaggaaatga agaaatacaa ttttgataga attattgcat ttggaggagg
     9361 aacaatagtt gatatatgta agatattagc acttgatgtt ccagaaaagt ctattgattt
     9421 atttgaagga gaagtatcac ctaaaaaagt aaaagagtta gtagtagttc ctacaacatg
     9481 tggtacaggt agtgaagtaa caaatgtagc tattgcagag ttaaaatcta aacatactaa
     9541 aaaggggctt gcagtagaag aaacatatgc agactatgca gtgttaattc cagagacagt
     9601 aaaagggtta ccttacaaat tctttgttac aagttcggta gatgctctta tacatgctat
     9661 tgagtcttat ctttctccaa aagcaagtcc atttacagag atgtattctc tacaagcaat
     9721 aaaaatgatt atggatgggt ataagaagat agttgataaa ggagaagaag agagatttaa
     9781 tcatcttaga gactttgtac ttgcatcaaa ttatgcagga attgcttttg gaaatgctgg
     9841 ttgtgcagca gttcatgcac tatcttattc aataggtgga gctttccatg ttgcacatgg
     9901 tgaagcaaat tatcagttct ttactgaagt atttaagatg tattctagaa aaaaacctaa
     9961 tggaaaaatt ataaaatgta caaaaatttt agcagatgcc cttgaatgtg accctaactg
    10021 tgatgtttat ggagaattag aaaagttttt aaataaatta atagctaaaa aagctttgag
    10081 agaatatgga atggtggaaa gtcagataga tgaatttaca gatagtacaa tagcaaatca
    10141 acaacgacta cttgctaata attatgtaga gcttagtaga gaggaaataa gagagatatt
    10201 tgcaaatctt tattaataca attttttatt tatatgtata gtcaacataa acaatgctaa
    10261 ttatatttat gtacaaataa aaataaggga acttaaattt taattaaagt taagttctct
    10321 tatttttata tggagttaaa tataagtata tttttaattt aaaatatgct tatatttaag
    10381 tctattactt tatttgaaac aatttgtaga atttcaaatt ccttgacagt aataagtaag
    10441 taattgtata atcaaactgc aatatattaa taatattagt gaaaatgaaa ggattataaa
    10501 atatgtataa atcagaaaat ttttcaaaaa aatctgtatt aataaattca tttttaagta
    10561 aatttttaaa aataattgga tggggtttgg taagcttatt tacattatca gccttagtac
    10621 tttcaactat ttccattata attggatggg ctgtaatact tcctattcaa gtatgtgcag
    10681 tatttttact tataggagga gtttgtattt ttaaaggtaa gagattagga gaccaaattt
    10741 ctcgttataa aaaatattgt acaattataa acaatagaaa tattattcca gtagaactta
    10801 ttgcagaaaa cacatctaaa tcattaaatt ttataataaa agatgtacaa aagatgatag
    10861 acaaaaacta ctttataaat acttatattg ataaaagaaa taatcagatt gtgttaacaa
    10921 atgaagattt tgttccacca agatatgaag atgtagcata tgaagtcaaa gaagaagaaa
    10981 agactgatga atccaatgaa gttgatacaa ttataaataa aggaatgaat tatctaacac
    11041 aaataaaaga agctaataaa aatattaaat cagaatcaat gggcgataaa attgtacaag
    11101 tagaagatgt tacaagtaaa atatttgatg ttgtaaaaca tgacccatca aaacttactc
    11161 agatacaaaa atttatggat tattatcttc caaccacttt aaaactatta aattcatatc
    11221 atacattgga agaacaagga attgatagag aaaatataac aacaactatg gaaagtattg
    11281 aaaatacaat gaatacgatt gtagtagctt ttgaaaatca attggactat ctgtttgaag
    11341 atgaagccat tgatatttct acagatatta ctgttttaga gaatatgtta gtccaagaag
    11401 gattaacgag tggtaaattt taacttcaat agaaaaaata ttctgttatt tttaaacaat
    11461 taattagtca taaagtataa aatgtgtatt atataagtta atcagtactt tatgaaggaa
    11521 gaggagagaa agatacaatg aataatttag atgatatacc agttatgcca actttgacac
    11581 ttgacccatt tggagaagaa tcaaacacta atgatataga taattctgac ttattaatga
    11641 aaaaggatga aaaagaccca gaagaagaaa aactgtctga atctgaacgc aagatggtaa
    11701 aggaatttgc ggaaaaaata gatattacaa atacaaatat gattttgcaa tatggagctg
    11761 gagctcaaaa aaaggtagca agtttttctg aaacagcttt aaagagtgta aaaacaagag
    11821 atttaggaga agttggagat atgcttacaa atctagttac tgatttaaaa gcattttcag
    11881 cagatgaaaa agaacaaagt ggttttttag gaatttttaa aaaggcaaat aataaaattt
    11941 caaatttaaa aactaagtat gatagtgcag aggtaaatgt ggataaagta agcaaagagc
    12001 ttcaaaagca tcaggttaaa atattgaaag atatagcaat gcttgataag atgtatgaat
    12061 tgaacttagc ttactcaaaa gagcttacta tgtatattat agcaggaaaa caaaaattaa
    12121 aggatatgaa agaatatgaa atgccaaagt tgagagaaaa agctcgttta tctggttcaa
    12181 cagaagatgc tcaatctgta aatgatatgg tttcactttg tgacagattt gaaaagaaga
    12241 ttcatgactt agaattaact agaatggtat cgcttcaaat ggctcctcag ataagattgg
    12301 ttcaaaatag taataatctg atggcagaaa agattcaatc aactatagta aataccattc
    12361 cattatggaa aaatcaaata gtgcttgctc ttggaatttc tcacttaaat caagctatga
    12421 aagcacagcg tgaagttagt aatatgacaa atgagctttt attaaagaat gcagaaactt
    12481 tgaagatggg aacaatagaa acagcaaaag aatcagaaag aggaattgta gatatagaca
    12541 caattaagaa aactaatcaa tctctaatat ctactattga tgaggtagtt aaaatacaac
    12601 atgatggtag aataaagaga caagaagcag aagttgaatt atcaaaaata gaaaatgaac
    12661 ttaaaagtaa gcttcttgaa tttacagtta aataaaaatt atactaatat aaaaagagac
    12721 tgtctcaaaa tagagataag tctctttttc attgtttaaa tgattaaagt atatttatat
    12781 tataaatttc tattatcttt taacataaca tcatgagctc caccttcaac tatactagta
    12841 gaagatacaa gtgtaagttt agcatttttt tgaagttcag gaatgctaag tgctccacag
    12901 ttacacatag tagaacgaac tttacttaaa gaaagagtta cattgtcttt caaagaacca
    12961 gcataaggca cataagagtc aacaccttct tcaaaagaaa gtttcttatc tccaccaagg
    13021 tcatatcttt gccagtttct agcacgagca gaaccttctc cccaatactc tttcatataa
    13081 ctaccattta tattaacttt gttagttgga ctctcatcaa aacgagagaa gtatctacca
    13141 agcattataa aatctgcacc catagctaaa gctaaagtta tatgatggtc ataaacgata
    13201 ccaccatcag agcaaattgg aatataaata cctgtttttt caaagtattc gtctctagct
    13261 tgagcaactt caattataga agtagcttgt ccacgtccta tacctttttg ttcacgagtt
    13321 atacatatag aaccaccacc aataccaact ttaacgaagt cagcaccagc ttcagctagg
    13381 taaaggaatc cttcgcggtc aactacattt ccagctccta ccttaactgt atcaccatat
    13441 ttttcacgaa tgaaatcaag tgttattttt tgccactcag aaaaaccttc tgaagaatct
    13501 atacataaaa catcagcacc agcttcaact aaagcaggaa cacgttcagc aaagtctcta
    13561 gtattaatac cagctccaac tacatatctc ttagaagaat ctaaaagttc aagtggattt
    13621 tctttatgag aagagtaatc tttacggaat accatataca taagattacc attatcatca
    13681 agtacaggaa gagaattaag tttgtggtcc catataatat tgtttgcttc ctttaaagta
    13741 acatctttgt tagcataaac tatagatgac ataggagtca taaattcact aactttagta
    13801 tctaaatcca tacgacttat acgataatca cgacttgcaa caatacccaa taatttacca
    13861 gttgaagttc catcttcagt aacagctact gtagaatgtc cagttttttc ttttaagtca
    13921 agtatgtctt ttaatgtgtt atcaggttta atatttgaat cacttacaac aaaaccagct
    13981 ttgtgggatt taacacgtgc taccatagca gcttcatttt caatagtttg agaaccgtat
    14041 ataaatgaaa taccaccttc tttagcaagt gcaatagcca ttttgtcatc agaaacagat
    14101 tgcataatag cagaagtcaa aggtatattc atataaatat ctgcttcctc gcccttttta
    14161 aatttagtaa caggtgtttt taaacttaca ttagcaggta tacaatcagt agaagagaaa
    14221 cctggtacaa gaaggtattc actgaaagta tgtgatggtg tatcaaaata aaacgccatt
    14281 ttttaatcac tccttatatt tttatacttt ttata
//
LOCUS       DAEOSP010000097        14298 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.097, whole genome shotgun sequence.
ACCESSION   DAEOSP010000097 DAEOSP010000000
VERSION     DAEOSP010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14298
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.097"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            75..1676
                     /locus_tag="KRH54_002205"
     CDS             75..1676
                     /locus_tag="KRH54_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBG8579862.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASNSNGQPPTITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLLVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSNEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            1895..3493
                     /locus_tag="KRH54_002206"
     CDS             1895..3493
                     /locus_tag="KRH54_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG8579863.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTIILVNTDNSIVDGLSASGLSGVAKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVVDQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNDLKANIKGNVDINKAGTYVLNISVVDNLGQKSEISVNVKVVVNASTKDSNS
                     YEFKAMVSNEMYDLVNSYRKEKGKKSLRELDSLAGMANAWSKYMEDKKVFAHEIDGKN
                     AAEVFFGFGARSGENIAYLPMNVKSVYTSKDAKEMAESIFDLWKKSSKYNENMLKEEF
                     YSFGFGMHVSSKGEVNATMEFLNS"
     gene            3783..5981
                     /gene="slpA"
                     /locus_tag="KRH54_002207"
     CDS             3783..5981
                     /gene="slpA"
                     /locus_tag="KRH54_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBG8579864.1"
                     /translation="MNKKNLAMAMAAVTVVGSAAPIFADTTVKEEGYTVVQDKYEKLL
                     KELKAKIKDGTITSVGVEFDGKPITTLAPKADGSDKDAIAEQLETLTKNQLKGLGDGK
                     YVDFKITYGAKAEVPAASLSADDIQKYADQINASEKILVEVAAGSEAGIAKFDSVNNK
                     VIAGDAPLKVKDAVKATVTTNGSNKKVLTISAAAGLSGFSYGTLKDTGASLSDVDAIT
                     LDTTNATITEGDTKVLDFDNSFKFNESTKKVGSLVTPNTTNTPADPGTKTTVRVIKAV
                     EKTIDVSSNSTTKAKDLAKQYVFTDVSDTDPESLSYMLKNINDGKVAVKNSDGDYEVT
                     IFPEGKRLNTLSASSAKTILGDKDTPAKIVLKASTTKKLADYIDDLITYNNSYSNVQT
                     VAGSDRIETAIELSRKYYNSTDKNALYGDPVNNVVLVGSQAIVDGLVASPLAAEKDAP
                     LLLSSKDKLDSSTRAEIKRVMDLNSSTGIKNNKEVFIAGGVNSISKDVENELKDMGLK
                     VTRLSGDDRYETSLAIADEIDINDKAYVVGGTGLADAMSIAPVASQIKDGEATPIVVV
                     DGKSDKLSKEAEDFLDDAQVDIIGGENSVSAKMEDYIDDATGKSPERISGADRQATNA
                     EVIKEYFDKDGVSNYFLAKDGSTKEDQLVDALAAAAVAGNYGSKHNEDGDITTDASPA
                     PIILATDNLSAEQHVAVSKTATTNGAKNLVQVGQGIADSVVSKLKDLLDM"
     gene            6227..8569
                     /gene="secA"
                     /locus_tag="KRH54_002208"
     CDS             6227..8569
                     /gene="secA"
                     /locus_tag="KRH54_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBG8579865.1"
                     /translation="MSVLDVILDKTDELEIKKLNNIVDKIDALEDKIQILDDEALKNM
                     TNVFKDKLSKGETLDDILPEAFAVVREVSKRILGMRQYRVQLIGGIVLHQGKIAEMRT
                     GEGKTLVGVAPVYLNALTGKGVHVITVNDYLAQRDKELMQPVYEFLGLTVGVIINGQQ
                     PVERKLQYQCDITYGTNNEYGFDYLRDNMVTQKSEKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGGGDEGTKLYELANAFIKNIRDEDFEMDRKDKTIALTASGLSKAEKFFGIEN
                     ITHIDNIEIFHHVNQSLRAHKLMNIDVEYVVRDNEILIVDEFTGRIMDGRRFTDGLHQ
                     AIEAKEGVKINDESKTMATVTFQNFFRLYSKLSGMTGTAKTEESEFEATYKINVVQIP
                     TNKPVIRADLHDKVFKTERAKYNAVVEEIIRINKTKQPVLVGTVSIEKSEKLSKLLKK
                     KGVKHEVLNAKQHDKEAEIISKAGKLGSVTIATNMAGRGTDISLGAGDKEEEEKVKEL
                     GGLYVIGTERHETRRIDNQLRGRSGRQGDPGTSRFFVSLEDEVIKLYGGKSIEKILKR
                     MNANEDDALESKALNRAIERAQKGIEGKNFEQRKNVLKYDDTINEQRKVIYDERNKVL
                     DGSDIEEEIQKMVMDIITEAAEKYLKKFRDYHGYFKYLYNTFMPADTLLIPDLDKKNV
                     EEIVNQTYQISKRVYDLKKMMIGIEELAQLEKRVLLQVVDTYWVDHIDAMDQLRQYIG
                     LKSYAQKDPFKEYAIEGYEMFEALNRNIRITTVQYLYKFN"
     gene            8983..10854
                     /locus_tag="KRH54_002209"
     CDS             8983..10854
                     /locus_tag="KRH54_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG8579866.1"
                     /translation="MNRKNLYVIMAAAMVSTSVAPSFAAEATQVKKQTITKKEATELV
                     SKVRELMAQRYTGGSQVGQPIYEIKVGETSSQLKIITNIDELEKLVNALGENKELLVT
                     IADKGHTTNSANEVVAEAIEKYENSADLSAEANSITEKAKTEATGIYGVATVEASYDS
                     TKDKLVITLKDKTDVVTSKTLQIGIGDEKVDLTANPVDIAGTNLDPSAEGFRVSKINK
                     LGVEGAKNIADIQLAEITIKNSDLNTVSPQDLYDGYRLTIRGNTIANSVGKSISDISS
                     KDSETGKYKFTVKYTDTSGKAIELTVESTNDKELKDVKDALNGNTKVNLIAGEDRYAT
                     AVAIAKETEYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKEI
                     IKKSPSAKIYIVGGESAVSNTARKQLESVTKNVERLAGDDRHTTSVAVAKAMGSFKEA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLTADAIKLMDGKEIGIVGGSNNVPSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKNDITNSQKNTLDKKLNLGAEVTQIGNGVELTVVQKIGKILGW"
     gene            10963..11670
                     /locus_tag="KRH54_002210"
     CDS             10963..11670
                     /locus_tag="KRH54_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG8579867.1"
                     /translation="MIEATLFFSPHQDDETLSMGSAIIEHVERSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESDFSKNRDEEFKNSCKAMGVKENNIHIENNRAH
                     DGELSKEKARDIMLKYLEKYPDAKVKTVTPYKALGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYNDFKKVNPNVRVWKVLSSQEEKLLNAMDAYKKWNPENGNYAIGYHSVK
                     SHFDELKSNKIQYVHAP"
     gene            11703..13835
                     /locus_tag="KRH54_002211"
     CDS             11703..13835
                     /locus_tag="KRH54_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG8579868.1"
                     /translation="MKISKRIMSLLTATFFIFALSINIADASSKESLVGSGRWETAIK
                     ISQNGWKDATNAILVNDNSIADALSATPFAKLKDAPILLTQSDKLDSRTKAELKRLGV
                     KNVYLIGGMNTLSTNIEKQLKSENMTFERISGNSRYETSLKLAERLDKEKSISDIVVV
                     NGEKGLADAVSVGSAAAQENMPIILSDPNNGIKVANSFISSKNIKKSYIIGGTYSVSS
                     SIEQSLPNVKRLSGNNRNETNAKVLEEFYKNTDLKNAYITKDGMKKQGDLIDSLAVGV
                     LAAKNSSPVVLVGNSLDSSQYDVLNTKSFDNIVQVGGNGNEDAVKDILDLQEETIYRV
                     KTADELDVALRKADANDIITFKPDDNKKITSSFSIETKKAITVELEGLYTQTITVNMP
                     NGELTNKGTLDGTVKIMDIKNGTFINEGDINDIDIYDPNGCKIENKSGGDIWVLTISS
                     SAKDVNIENKGDISKVTNNSTSVIIRNSGNIDTLSGINAPAISGNKPRVDDTDKDEKR
                     ASGIYPTSEPLSPARAGYISVKIFTKPEDTNYKIYYRVVSSRPSALYIGDRIDTSDWT
                     AVPSSVLPFTIKAENGYYIEAVEINSDTKKVSRWGKSDYIDDGFYLKSETAKGLSADI
                     DFDGDYVKLTATGADSGCTVYYRIVSKKPVSMNVGDKVTISDWDEITTNRVRIEANDV
                     KDKYIELVELDDSTNKVTRWGASDKVVE"
     gene            complement(13888..14283)
                     /locus_tag="KRH54_002212"
     CDS             complement(13888..14283)
                     /locus_tag="KRH54_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBG8579869.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIELIFLFKEFLFFVSFRLLSGIIEMIIMYIMIDLFF
                     VNDITVKVFTNIIVIVLNYLFSKMIIFRK"
BASE COUNT         5827 a         1565 c         2732 g         4174 t
ORIGIN      
        1 tatggaaaat aaagagaaaa taaatggaaa ataatcacaa tataaatata aaatataaaa
       61 atcatcaggg ggatatgaaa atgaacaaaa aaatattatc attaggtcta gcagtatcat
      121 taatcttagt aaacttcaaa agtgtaaatg catcatcagt agtagaaaaa atatacggta
      181 aagatagata tgaaacagca gcaaagatag ctgataaaca gacttatgaa acagtaattt
      241 tagtaaatac agaaaaatca cttgcagatg gattaagtgc aagtggatta tcgggagcta
      301 caaaagctcc aatactattt acacaacaaa ataagatacc agcagacaca aatagatgtc
      361 taaaaaatat caaaaaagca tatataatag gtacagaaga tactataagt aaatcagtag
      421 aaaaagaact agattctaaa aatatagaag tcaaaagaat tggtggagaa gatagactaa
      481 aaacaagcta tttaatagct aaagaaatag caactataaa aaaagtggat aaagtactat
      541 taactaatgc atatagtgga gaagcagatg caatgagtgt atcatcagta gctactagag
      601 atggagctcc aatcatactt acagatggaa agagtgtgcc ttttgatgta aaaaatattc
      661 aatcatattg tataggttca gaggagataa tgagtaatcc tctagttaaa aatacaaact
      721 cagtaagaat agaaggaact gaccgatttg aaactaacaa aaacgtaata gactactttt
      781 ttaatagtgc agatggattc tatgtatcag atggatacca attagtagat gcaatagcag
      841 ctgcaccact tactaaaaac tctccaatgg tactagtaaa tgatggaagc gataaaactg
      901 tattagaagg agctaagaat ataacctctg taggtgaaat aaatgagaag gtaatacaac
      961 agtgtataaa tgcttcaaat tcaaatgggc aacccccaac aattacagtt ggaagtacag
     1021 aggtatataa gggtgaaaag tttgacactg gcaagttaaa tatagtagct aaagataaca
     1081 cagggaaggt attaccaata gaagttgatg ggtttataga tacaaataga gtaggtacat
     1141 acatattgac attaaaagct acagacgaat ggggaaaaag cacaggaaaa agagtagaaa
     1201 taaaagtgtt ggatgacaaa tctcatgatt acaacagccc agaatttaaa aaaatggtat
     1261 ctactgaaat gtataactta atcaattcct atagaaaaga aaaaggcaaa gagcctttgc
     1321 tagtgtctag taggctagaa ggtatggcaa atgcatggtc taagtatatg atggataaaa
     1381 aagtatttgc acattatata gatggaaaaa atgctccaca agtattttct gaatttggaa
     1441 tgagaagtga ggaaaatata gcatatattt atatagattc taagaacgtt caaacgacac
     1501 aagatgcaaa agacttggca aaggctatat ttgaggtgtg gaagaagtct ccagagtaca
     1561 atgcaaacat gcttagtaat gagttttata gtactggatt tgggctttat atattatctg
     1621 atggtcaagt gcatgctact caggagtttt taaatggaaa tgaaggtagc ttgtaaaaaa
     1681 ttccataaat cttgtttttt tgttaattta ctgatattat aaaatgaaaa tcacatctaa
     1741 agtacttttt gtaatctgtt ttgttattgc tttacaggct atggtatttt aggtgttttt
     1801 tgttttagta gtttatagat tttgagggaa tcactactga ggatgtaatt tatgattagc
     1861 tgaattatat aaaaaaatta gtaggaggag tttgatgaag gtaaataaga gagtattatc
     1921 aataggattg acaatatcat taataatggc aggagcacca aatatcaatg cactttcgag
     1981 catagaaaag atacagggga aagatagata tgagacttct gcattgatag cagataaaca
     2041 aatatatgat acaatcatat tagtaaatac tgataattct atagtagatg gattaagtgc
     2101 aagtggatta tctggagttg caaaagctcc tataatgttg gtgcaaagag ataagatacc
     2161 aacagatgtt gaaaaaagac taaaggatgt taagaatgct tatgttatag gtacagagga
     2221 tacaataggt aaatctgtag aaaatcaact taagaataaa gggattgaag ttaaaagaat
     2281 tggtggggaa gatagaataa agactagcta tcttatagct aaggagattt cagctattaa
     2341 accagtaaat gatggtgata aggtttttct tgtaaatgga tacacaggtg aggctgatgc
     2401 aatgagtgta tcttctgtag ctgctagaga tggagttcct gtgatactta ctgatggaaa
     2461 gagtatacca tttaaagttg atggcgttca gtgctattct cttgggtcag aggagataat
     2521 gagtaatgaa cttgtgagta agactaattc agttaggatt gctggtaagg ataggtttga
     2581 aactaataag aaggttatac aacgctttta taaaggaaca aagaagtttt atgtttctca
     2641 agggtataag ttagttgatg ctgtggctgg ttctccgttg gctaaggaca aacctatagt
     2701 acttgttaat gatggaagtg ataagagtgt gcttagaggt gctgatgaag ttacttcttt
     2761 aggtggaatg gataagaagg ttgttgacca atgtattagt tctgcatctg ataaaaatac
     2821 aatgcctaca attactgcta atgatgtaga gatttctgta ggggataagt ttgataatag
     2881 tatgttgaat atagttgcta ctgattatta tggaaatgat ttaaaggcaa atattaaagg
     2941 aaatgttgat attaacaagg ctggaacata tgttttaaat ataagtgtag tggataattt
     3001 aggtcagaag agtgagatta gtgtaaatgt taaggtagtt gtaaatgcga gtacaaaaga
     3061 ctctaatagt tatgagttta aggctatggt atctaatgag atgtatgatt tagttaattc
     3121 atatagaaaa gaaaaaggca agaagagttt aagagagtta gactctttag ctggaatggc
     3181 aaatgcatgg tctaaatata tggaagataa gaaggtattt gcccatgaga ttgatggtaa
     3241 aaatgcagct gaagtatttt ttggctttgg agcaagaagt ggtgaaaata ttgcatatct
     3301 accaatgaat gttaaatctg tttatacttc taaagatgca aaagaaatgg ctgagtctat
     3361 ttttgattta tggaagaagt cttctaagta taatgaaaat atgttaaaag aagaatttta
     3421 tagctttgga tttggtatgc atgtttctag taaaggagaa gtgaatgcga caatggaatt
     3481 tttaaatagt tagattttat attaatttac attggaaaag atatagtaaa tttgtaaaaa
     3541 taaatatata aaattaaaat aacttataaa attataaaaa ataatttaaa aataataaaa
     3601 atgatgaaaa aacaattaat ttgttggaaa aaacttcaat taattttgca taaattattg
     3661 aatttatatt ttatatagtc tatcataaga gtgtgagaaa tatttggatt atatgaacat
     3721 ggaaatttaa tgtacataat taaagagatg taattaatat aatgttggga ggaatttaag
     3781 aaatgaataa gaaaaattta gcaatggcaa tggcagctgt tactgttgta ggttctgctg
     3841 ctccgatatt tgctgatact actgttaaag aagaaggata tacggtagta caagacaaat
     3901 atgaaaaatt acttaaagaa ttaaaggcaa agataaaaga tggaactatt acatcagttg
     3961 gtgttgaatt tgatggaaaa ccaattacta cattagcacc aaaggcagat ggtagtgata
     4021 aagatgcaat agcagaacag ttagaaactc ttactaaaaa ccaacttaaa ggtttaggtg
     4081 atggaaaata tgtagatttt aaaattactt atggtgctaa agcagaagtt ccagctgctt
     4141 cattaagtgc agatgatata caaaaatatg cagatcaaat aaatgcttct gagaaaatat
     4201 tagttgaagt agcagcagga tctgaagcag gtatagcaaa atttgattct gttaacaata
     4261 aagttatagc aggagatgct cctcttaaag ttaaagatgc agtgaaagcg actgttacta
     4321 ctaatggtag taataaaaaa gttcttacaa tatcagctgc agctggatta tcaggatttt
     4381 catatggaac acttaaagac acaggtgcaa gtttatctga tgttgatgca ataactcttg
     4441 acactacaaa tgcaacaata acagaaggtg atactaaagt attagatttt gataattcat
     4501 ttaagtttaa cgaaagtact aaaaaagttg gtagtttagt tacaccaaat acaacaaaca
     4561 ctccagcaga tcctggtact aaaactactg ttagagtgat aaaagctgtt gaaaaaacaa
     4621 tagatgtaag ttcaaattca actacaaaag ctaaagattt agctaaacaa tatgtattca
     4681 cagatgttag tgatactgat ccagaaagtt taagttatat gcttaaaaac ataaatgatg
     4741 gaaaagttgc tgttaaaaat tctgatggag attatgaagt aactatattc ccagaaggaa
     4801 aaagattaaa tactttatct gcttcttcag ctaaaactat tttaggagat aaagatactc
     4861 cagcaaaaat agttttaaag gcttcaacaa caaagaaatt agcagactat atagatgatt
     4921 taataactta taacaatagt tattcaaatg tacaaactgt agcaggtagt gatagaatag
     4981 agactgctat agagctaagc cgtaagtact ataactctac tgacaaaaat gctttatatg
     5041 gagatcctgt aaataacgtt gtattagtag gttctcaagc tatagttgat ggattagtag
     5101 catctccttt agcagcagaa aaagatgctc ctttattgtt aagttctaaa gataaattag
     5161 attcatcaac aagagctgag ataaaaagag ttatggactt aaattcttca acaggaataa
     5221 aaaataataa agaagttttc atagcaggtg gagtaaattc tatatctaaa gacgttgaaa
     5281 atgaactaaa agatatggga cttaaagtaa ctagattatc aggtgatgat agatatgaaa
     5341 cttcattagc gatagctgat gaaatagata taaatgataa agcttatgtg gtaggtggaa
     5401 caggattagc agatgctatg agtattgctc cagttgcatc tcaaataaaa gatggtgaag
     5461 ctactccaat agtagttgta gatggaaaat ctgacaagtt atctaaagaa gctgaagatt
     5521 tcttagatga tgcacaagta gatataatcg gtggagaaaa tagtgtttct gctaaaatgg
     5581 aagattatat agatgatgct actggaaaat ctccagaaag aattagtggt gctgatagac
     5641 aagcaactaa tgctgaagtt ataaaagaat actttgataa agatggagtt tcaaattact
     5701 tcttagcaaa agatggttca actaaagagg atcagttagt tgatgcatta gcagcagcag
     5761 cagtagctgg aaactacggt tctaagcata atgaagatgg agatataact acagatgctt
     5821 ctcctgctcc aattatttta gcaactgata atttatcagc tgaacaacat gtagctgtaa
     5881 gtaaaactgc tactactaat ggtgctaaga acttagtaca agtaggtcaa ggaatagctg
     5941 attcagttgt tagcaaatta aaagatttat tagatatgta atataaattt tatatattga
     6001 ataaaaaagg tttctctttt gagaaacctt ttttatttta aacataaatt tttataaata
     6061 aaaagaggct aattagcctc tttttaatgt gagaaacact taaaaaataa gatgtaatta
     6121 tttatttaag tatcattatt atactatacc ttaaaataaa tgtctactat tctataattt
     6181 taaggtataa tagtaagtgg aaaataaata aggaggtaaa ttaaatatgt cagttttaga
     6241 tgtaatatta gacaaaacag atgaattaga aattaaaaaa ttaaataata tagtagataa
     6301 aatagatgct ttagaagata aaatacaaat actagatgat gaagcattaa aaaatatgac
     6361 gaatgtattt aaggataagt taagtaaagg agaaacctta gatgacatat tgccagaagc
     6421 atttgctgtt gtaagagaag tatcaaaaag aatattaggt atgcgtcaat atagagttca
     6481 attaattggt gggattgtat tacatcaagg gaaaatagca gagatgagaa ctggagaagg
     6541 aaagactcta gtaggagtag ctccagttta cttgaatgca ttgactggta agggggtaca
     6601 tgtcatcacg gttaatgatt acttggctca acgtgataaa gaacttatgc aaccagtata
     6661 tgaattttta ggtttaacag taggagtaat tattaatggg caacagccag tagaacgaaa
     6721 attacaatat caatgtgata ttacttatgg tacaaacaat gagtatggat tcgattattt
     6781 aagagataat atggtaacgc aaaaatcaga gaaagttcaa agagaactaa actttgctat
     6841 agttgatgag gttgattcaa tacttataga tgaagctaga acaccactta taattgctgg
     6901 tggaggagat gaaggtacta agctgtatga acttgcaaat gcttttataa agaatataag
     6961 agatgaagat tttgaaatgg atagaaaaga taaaaccata gcacttactg caagtggtct
     7021 tagtaaagct gagaaatttt ttggaataga aaatataact catatagata atatagaaat
     7081 ttttcaccat gtaaatcaat ctctaagagc acataaatta atgaacatag atgttgaata
     7141 tgttgttaga gataatgaga tactaattgt agatgaattt acaggaagaa taatggatgg
     7201 aagaaggttt actgatgggc ttcatcaagc aattgaagct aaagagggag taaaaataaa
     7261 tgatgaatca aagaccatgg caacagtaac atttcaaaat ttctttagat tatactctaa
     7321 attatcagga atgacaggta cagcaaagac agaggaaagt gagtttgaag ctacatataa
     7381 gattaatgtt gttcagatac caactaataa accagttata agagctgatt tacatgataa
     7441 agtattcaaa acagaacgtg caaagtacaa tgctgttgta gaagaaataa taaggataaa
     7501 taaaactaag caaccagtgc ttgtaggaac tgtttcaatt gaaaaatctg agaaattatc
     7561 taagctactt aaaaagaaag gtgtaaagca cgaggtctta aatgctaaac aacatgataa
     7621 ggaagctgaa ataatatcta aagctggtaa attaggttct gttacaattg ctactaatat
     7681 ggcaggtaga ggaactgata tatcacttgg agctggagat aaagaagaag aagaaaaggt
     7741 gaaagagtta ggtggcttgt atgttatagg tactgaaaga catgagacaa gaagaattga
     7801 taatcagctt agagggcgtt cgggaagaca aggtgaccct ggaacttcta gattctttgt
     7861 aagtttagag gatgaagtaa taaaattgta tggtggaaaa agtatagaaa aaatattaaa
     7921 gagaatgaat gctaatgaag atgacgcgtt agaaagtaag gctttgaata gagcaataga
     7981 aagagcacag aaaggcattg aaggtaaaaa ttttgaacaa agaaagaatg ttcttaagta
     8041 cgatgatact attaatgagc aaagaaaagt aatatatgat gaaagaaata aggtattgga
     8101 tggttctgat attgaagaag agatacaaaa aatggttatg gatattatta cggaggctgc
     8161 tgaaaagtac ttaaaaaaat ttagagacta tcatggatat tttaagtatt tatataatac
     8221 ttttatgcca gctgatactt tattaattcc agacttagat aaaaaaaatg ttgaagaaat
     8281 agtaaatcaa acatatcaga tttcaaagcg tgtttatgat cttaagaaga tgatgattgg
     8341 aattgaagaa ttggctcaat tagagaaacg agtattactt caagttgtgg atacttattg
     8401 ggttgaccat atagatgcaa tggatcaact aagacaatat ataggtctta aatcttatgc
     8461 acaaaaagat ccatttaaag agtatgctat agaaggttat gaaatgtttg aagctttaaa
     8521 taggaatata agaataacaa cagtgcaata tctatataaa tttaactaaa aaagtgaccc
     8581 attgggtcac tttttatata ttaagattaa agtgaaattt attttacagt acacactcat
     8641 atagataaat atttataata atgaaaaaga ggctgaacgt cagcctcttt tcctgtgtga
     8701 gaaatacgga aaaaagagat gcttgaaatt tctttcttcc atttaatatt atactataaa
     8761 gagaataaaa agtccatgag ttatctttat gattaaaaaa aaaaattaca attttgttac
     8821 tttttagtat acaataataa atttaaattg actttgttaa tttttattaa taaaatatcc
     8881 aaatattaca atatctacag tttattgaaa taatgtgtaa attatattat aattggtaaa
     8941 taaggaaaaa tggtgaaaac caaattttgt agggggaaat atatgaatag aaaaaatctt
     9001 tatgtaatta tggcagctgc aatggtaagt acatcagtag ctccaagttt tgctgcagaa
     9061 gctacacaag taaagaaaca aacaataact aaaaaggaag caacagaatt agtttctaaa
     9121 gttagagaac taatggctca gagatataca ggaggttctc aagttggaca accaatatat
     9181 gaaataaaag ttggtgaaac ttcatcacaa ttaaaaataa taactaatat agacgaatta
     9241 gagaaattag taaatgcttt aggagaaaat aaagagcttc ttgtgactat agcagataaa
     9301 ggacatacaa caaatagtgc aaatgaagtg gttgctgaag caattgaaaa atatgaaaat
     9361 tcagcagacc tttcagctga agctaattca ataacagaaa aagctaaaac tgaagctact
     9421 gggatttatg gagtggcaac tgtagaagct tcatatgaca gtacaaaaga taagttagta
     9481 ataactttaa aagataagac tgatgtagta acttctaaaa cgctacaaat aggtattggt
     9541 gatgaaaagg ttgatttaac agcaaatcca gttgatatag caggaaccaa tttagaccct
     9601 tctgcagaag gatttagagt aagtaaaata aataaattag gtgtagaagg agctaaaaat
     9661 attgctgata ttcaattagc tgaaataact ataaaaaata gtgatttaaa tacagtttca
     9721 cctcaagatt tatatgatgg atatagattg actattcgag gtaatacaat agcaaatagt
     9781 gtaggaaaat caattagtga tatttcatca aaagattcag agacaggaaa gtataaattt
     9841 actgttaaat atactgatac atctggaaaa gcgatagaac ttactgtaga aagtactaat
     9901 gataaagagt taaaagatgt taaagatgcc ttaaatggaa atacaaaagt caatttaata
     9961 gctggggaag acaggtatgc aactgctgta gctatagcaa aagaaacaga atatactgac
    10021 aatatagtta tagttaattc aaataaatta gttgatggat tagcagcaac accactagct
    10081 caatctaaaa aagctcctat attgttggcg tctgataatg aaataccaaa agtaacttta
    10141 gattatataa aggaaataat taaaaaaagt ccatcagcta aaatatatat agtaggtgga
    10201 gaatcagcag tatcaaatac agctagaaag caattagaat cagtaactaa gaatgttgaa
    10261 agattggctg gagatgatag acatacaact tcagtagcag tagcaaaagc tatgggttct
    10321 tttaaagaag catttgtagt aggtgcaaaa ggggaagctg atgctatgag tatagctgct
    10381 aaagctgctg aacttaaggc tcctataata gtaaatggtt ggaatgatct tacagcagat
    10441 gctatcaagt tgatggatgg aaaagagatt ggtatagttg gtggttctaa taatgtacct
    10501 agccaaattg aaaatcaact tgctgatatt gataaagata gaaaggttca aagagttgaa
    10561 ggtgaaacaa gacacgatac taatgctaaa gttatagaaa catattatgg caaattagat
    10621 aaactatata tagcaaaaga tggatatgga aataatggta tgttagtaga tgcattggca
    10681 gcaggtcctc tagcagcagg aaaaggacca attcttttag ctaaaaatga tataacaaac
    10741 tcacaaaaga atactttaga taaaaaacta aatcttggtg cagaagtaac tcaaataggt
    10801 aatggggttg aattgactgt agttcaaaaa ataggcaaaa tactaggatg gtaataatca
    10861 ataatataga aaaggattcc ttattattta taggaatctt tttctatcga cataatattc
    10921 attaaagtat aacatgctct tgacagatag gaggatatga tagtgataga agcaacattg
    10981 tttttttccc cacaccaaga tgatgaaaca ttaagtatgg gtagtgcaat aattgaacat
    11041 gtagaaagaa gcgatacaca tgttatatta tgtactgatg gcagtaaatc aataataaga
    11101 aaagtattag atgatggagg gtcatgttct tatcatatta aagatgtaca caagtactct
    11161 ttatcagaaa gtgatttttc aaaaaataga gatgaagaat ttaaaaatag ttgtaaagca
    11221 atgggagtta aagaaaataa tatacatata gaaaataata gagctcatga tggagaattg
    11281 agtaaggaaa aagcaagaga tataatgcta aaatatttag aaaaatatcc tgatgctaaa
    11341 gtgaagaccg ttacaccata taaagctctt ggaatacatg aagaccatag agcattaggt
    11401 gaagctgcat tagaattgta tagagaagga aaaataaaag atttaagatt ttatgtagag
    11461 ccatacgatt ataatgattt taaaaaagtt aatccaaatg ttagagtatg gaaggttttg
    11521 agtagtcaag aagagaaatt attaaatgct atggatgcat acaagaaatg gaatcctgaa
    11581 aatggtaact acgcaattgg atatcattct gtaaaatctc actttgatga attaaaatcc
    11641 aataaaatcc aatatgtaca tgctccataa acattaatat aaaaactagg aggatatagt
    11701 taatgaaaat atctaagaga ataatgtcgt tgttgacagc aacttttttt atctttgcat
    11761 tatctatcaa tatagctgat gcttcatcaa aagagagtct agtgggctct ggtagatggg
    11821 aaactgcaat aaaaattagt cagaatggat ggaaagatgc tactaatgct atactagtaa
    11881 atgataattc tatagctgat gcattatctg caactccatt tgctaagtta aaagatgcac
    11941 ctattttatt aactcaaagt gataaattgg atagtagaac taaagcagaa ttaaaaagac
    12001 taggtgtaaa aaatgtatat ttaattggag gtatgaatac tctaagcacc aatattgaaa
    12061 aacaattaaa atcagaaaat atgacttttg agaggatttc aggtaatagt agatatgaaa
    12121 catctttaaa attagctgaa agattagata aagagaagag tatttctgat atagttgtag
    12181 ttaatggaga gaaagggcta gcagatgcag taagtgtggg ttctgctgca gcacaagaaa
    12241 atatgccaat tatactttca gatcctaaca atggaataaa agttgcaaat agctttatta
    12301 gtagtaaaaa tataaagaaa tcttatataa ttggtggtac atattctgta tctagttcta
    12361 ttgaacagag tttacctaat gtaaaaagat tatctggaaa caatagaaat gaaactaatg
    12421 caaaggttct agaagaattt tataaaaata cagacttaaa aaatgcttat ataactaaag
    12481 atggtatgaa aaaacagggg gatttgatag attctctagc tgtaggtgta ttggcagcta
    12541 aaaatagctc tccagttgta ttagtaggaa attcacttga ctctagccaa tatgatgtgt
    12601 taaatactaa aagttttgat aatattgtgc aagttggtgg aaatggaaat gaagatgctg
    12661 taaaggatat tcttgattta caagaagaaa ctatatatag agttaagaca gcagacgaat
    12721 tggatgttgc tctaagaaaa gcagatgcta atgatattat aacatttaaa cctgatgata
    12781 ataaaaaaat tactagttca tttagtatag aaacaaagaa agctataact gtagagcttg
    12841 aaggattgta tacacaaaca ataactgtaa atatgccaaa tggagaatta actaataagg
    12901 gtacattaga tggaactgtg aaaataatgg atataaaaaa tggcacattt atcaatgaag
    12961 gtgatataaa tgatattgac atttatgatc caaatggatg taaaatagag aataaaagtg
    13021 gtggagatat atgggtttta actatatcta gtagtgctaa agatgtaaat atagaaaata
    13081 aaggtgatat aagtaaggtt acaaataatt ctactagcgt tataataaga aattcaggaa
    13141 atattgatac tttaagtgga attaatgcac cagcaataag tggaaataaa cctagagttg
    13201 atgataccga taaggatgaa aaaagagcaa gtggaatata tcctacttca gaacctttat
    13261 cacctgcaag agctggttat ataagtgtaa aaatattcac taagcctgaa gatacaaatt
    13321 ataaaattta ttatagagta gtaagcagta gaccatctgc attatatatc ggagatagaa
    13381 tagatacaag tgactggact gctgtaccat cttctgtttt accatttact attaaagcag
    13441 aaaatggata ttatatagaa gctgttgaaa taaattctga tactaagaaa gtatctagat
    13501 gggggaaatc tgactatata gatgatggat tttatttaaa atcagaaact gcaaaaggat
    13561 taagtgcaga tatagatttt gatggagatt atgttaaatt aacagcaaca ggtgctgatt
    13621 ctggatgtac agtttattat agaatagtat caaaaaagcc agtatcaatg aatgttggag
    13681 ataaagtaac tatatctgat tgggatgaaa ttactactaa tagagtgagg atagaagcaa
    13741 acgatgtaaa ggataaatat atagaactag ttgaattgga tgattcaact aataaagtaa
    13801 ctagatgggg agcaagtgat aaggtagtag aataggtatc taatgtgaaa cataaaaaca
    13861 gactgattaa taatgggcag tctgttttta tttcctaaaa attatcattt tactaaataa
    13921 ataatttaaa actataacaa ttatattagt aaatactttt acagtaatat catttacaaa
    13981 aaataaatca atcattatat acataataat catttctata ataccagaaa gtagcctaaa
    14041 actgacaaaa aacaaaaact ccttaaacaa aaatattagt tcaattcttt tactctcaaa
    14101 cacaaaaaac ttattagtaa cataagcaaa taaaacagca agtatccatg ccaaagcatt
    14161 tgcaaccata aaattaaata aaataactct agtaaaaaat aaatacgaaa ctatatttac
    14221 aagagtagta aatgctccaa aaaataaata taatatcgtc tctctatgct tttttaaaat
    14281 caaatataat cctcctaa
//
LOCUS       DAEOSP010000098        14208 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.098, whole genome shotgun sequence.
ACCESSION   DAEOSP010000098 DAEOSP010000000
VERSION     DAEOSP010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14208
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.098"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            506..1399
                     /locus_tag="KRH54_002213"
     CDS             506..1399
                     /locus_tag="KRH54_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBG8579870.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1399..2793
                     /gene="gltA"
                     /locus_tag="KRH54_002214"
     CDS             1399..2793
                     /gene="gltA"
                     /locus_tag="KRH54_002214"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBG8579871.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            3159..5378
                     /locus_tag="KRH54_002215"
     CDS             3159..5378
                     /locus_tag="KRH54_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG8579872.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5525..6271
                     /locus_tag="KRH54_002216"
     CDS             5525..6271
                     /locus_tag="KRH54_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8579873.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6271..8151
                     /locus_tag="KRH54_002217"
     CDS             6271..8151
                     /locus_tag="KRH54_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8579874.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKNYTRKVWF
                     FD"
     gene            8270..9661
                     /locus_tag="KRH54_002218"
     CDS             8270..9661
                     /locus_tag="KRH54_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8579875.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9768..10808
                     /locus_tag="KRH54_002219"
     CDS             9768..10808
                     /locus_tag="KRH54_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG8579876.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISTDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11297..11950
                     /locus_tag="KRH54_002220"
     CDS             11297..11950
                     /locus_tag="KRH54_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBG8579877.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVNASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            12026..12235
                     /locus_tag="KRH54_002221"
     CDS             12026..12235
                     /locus_tag="KRH54_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579878.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12375..12941)
                     /locus_tag="KRH54_002222"
     CDS             complement(12375..12941)
                     /locus_tag="KRH54_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBG8579879.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            13102..13755
                     /locus_tag="KRH54_002223"
     CDS             13102..13755
                     /locus_tag="KRH54_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBG8579880.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(13913..14089)
                     /locus_tag="KRH54_002224"
     CDS             complement(13913..14089)
                     /locus_tag="KRH54_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579881.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         5571 a         1402 c         2383 g         4852 t
ORIGIN      
        1 tttatataaa aattcaaata attaatgaat attgaaaatt ttagatatat aataaaataa
       61 gagtatattt tatacaatat gaaagctggt attgcaatag attaaagcct tatacacgtt
      121 tagtttttaa agaaattagt gttattatga aaacctaagt taatttatta aataaataat
      181 tgaataaaaa aattttatta tgtatttata aaatttttgt tacaaatttg gcataatata
      241 gtatacttta agtgtgtatt aggttaaatc tataacaaat tagacgtatg atatagactt
      301 agtgtttatt ggcatttaca agaaatttgt taatatcaac tatttgtagt ttgggcagtt
      361 ataattatta taggggggat gtccaataca aaacaatcaa ccaagaacta atattatatt
      421 ataaagagtt atagttttgt gtgtttatgt ttttaattaa atttaaagtg ggaaaaatat
      481 atttgttaga atggagaata gcgagatgag taataaaata gttagtaaga gacaattaac
      541 agatagtatt tatttaatgg aaatagaggc acctagagtt gcaaaatctt ctcaaccagg
      601 tcaatttata ataattaaaa atgatgaaaa aggagagagg attcctctta caatagctga
      661 ctatgataga gaaaaaggaa cagtaactat tgtattccaa acagttggtg cttcaactaa
      721 aaagttagca atgtttgaag aaaacgattt tgtaatggat tttgtagggc cattaggaca
      781 agcaagtgaa tttatacatg aagatataga agaattaaga aataaaaaga tattatttgt
      841 agctggtgga gttggttctg ctccagttta tccacaagtt aaatggttta aagagcatgg
      901 attagatgta gatgtaataa ttggtgcaag aactaaagaa ttaattatat tagaagatga
      961 tatgaaaaaa gtagctaaaa atgtatatgt atcaacagat gatggtacat atggatttaa
     1021 tggaagagtt acagacctat taaaagattt agttgataat caaggtaaaa aatacgacca
     1081 agcaatagta attggaccaa tgataatgat gaaatttatg tgccaattaa ctaaagaact
     1141 aaatatacct actatagtaa gtttaaatac aataatgata gatggaacag gtatgtgtgg
     1201 tggttgtaga gttagtgttg gaaatgaaac taagtttgca tgtgtagatg gtcctgaatt
     1261 tgatggtcat ttagttgatt ttgatcaagc aatgagaaga cagtctatgt acaagactca
     1321 agagggaaga gctatgctaa aacttgaaga aggcgatagt catcatcata gtaattgtgg
     1381 atgtggaggt aacaaataat ggatgctaaa aaagtaaaag taccagttag agaacaagaa
     1441 cctgcagtga gagcaacaaa ttttgatgag gtatgcttag ggtataataa agaagaagct
     1501 atggcagaag ctaatagatg tttagcttgt aaaaaaccaa aatgtgtggg tggatgtcca
     1561 gtaggaatcg acataccagg gtttataaca aaaattaaag aagatgatat agaaggtgct
     1621 gctaaagtaa tagctaagag cagttcatta ccagctgttt gtggtagagt atgcccacaa
     1681 gaaagtcaat gtgaaggtgt atgtatactt ggaataaaat cagatgctgt atcaataggt
     1741 aaattggaaa gatttgtagc agattggtca aaagaaaatg acataaattt atctgataca
     1801 gagcctaaga aaaatcaaaa agtagcagta ataggaagtg gtccagcagg tctagcttgt
     1861 gctggtgatt tagctaaaaa aggctatgat gtaacaatct ttgaagcaat gcatgagcca
     1921 ggtggagttt taacttacgg aatacctgag tttagacttc caaaacaagc tgttgttcaa
     1981 cctgaaatag acaatataag aaaattaggt gtaaaaatag aaactaatgt aattgttggt
     2041 aaaacaatta cagtagatga acttatagaa gatgaaggat ttgaagctat attcataggt
     2101 tcaggagctg gtcttccaat gtttatgaat ataccagggg aaaatgcaaa tggagtattt
     2161 tctgcaaatg agtttttaac tagagtaaac ttaatgaagg cttatagaga tgattatgat
     2221 acacctatta gttctggtaa aaaagttgct gtagtaggtg gtggaaatgt tgctatggac
     2281 gctgctagaa ctgcattaag attaggttca gaatcatata tagtttatag aagaagtgaa
     2341 aaagagcttc cagcaagagc tgaagaagtt catcatgcaa aagaagaagg cattatattt
     2401 aatacattaa caaatcctaa ggaaatttta gtagatgaaa atggatatgt taaaggtatg
     2461 gtttgtataa gaatggaatt aggagagcca gatgattctg gaagaagaag acctatagaa
     2521 atagaaggtt ctgaatttgt acttgatgta gacacagtta taatgtcact tggaacaagc
     2581 ccaaatccac taatatcttc tacaacaaag agtcttgata taaataagaa gagatgctta
     2641 ataacagatg aaaatggtca gacttctaaa gagggtgtat ttgcaggtgg agatgctgtt
     2701 actggagcag caacggtaat atctgcaatg ggtgctggta aaacagcagc agcatcaata
     2761 gatgaatatt taaaagcaaa agttaatgct taatttgtaa attaaagaga gggtgtctca
     2821 aaatgaactt tttagttcat gagacactct ttttgtacaa aatcaaatat gttttaatca
     2881 tttcaacaat gaaaatttca ttcctgtaga aagtctaact tttaggtctt agtacaatct
     2941 ctattttgat gtagccactt ttttagatgg tttaataatt tatcagttat tattgtaata
     3001 tttgatacta agtaacataa ctttgtctaa ctatgataaa atagttatac aataaaggtc
     3061 ttgtaaggag tgaataatgt gtggctaata aaatcagatt tgtcaaatct aacattaaat
     3121 aatagaaaaa taaattgtgt aattaatggt attttaatat gaaaaaaaga gaaaaagtat
     3181 ttgatgggct agtttttaag catatattaa tcttattaat actattagtt ttgattgatt
     3241 taacaagtac catcatatat gcaagtaact ctatggaaat tacttctaga aatatgattg
     3301 aggcatcaaa aagagaactt gaaaactatt tagaagtaaa catatcttta ttaaaagctc
     3361 ttagtcaaga tgatagattt tctaatggtg aaacatcact aattgaaaag ggaaaattat
     3421 taagtccata tcaaaaggaa tataatcttt ttatgattgg tataacagat acaaaaggaa
     3481 atgcttcaag tacatatagg gaaaatgtta actcaataaa agataagcct agttttgaga
     3541 aagcaattaa aacaaagcaa gtagtagttt cagatattga agtcagtaat gtaacaggtg
     3601 acaaagtatt tatcatatat gtaccaattg taaaaaataa tgagatgatt gggaccattt
     3661 ttgcttcttt ttattttcaa gatgtaaata atttaataag tagaagcaat tttgatgatt
     3721 ctattaaatt tctaatgata gataaagatt atactattat ttctcatcca aacaaaaaat
     3781 atgtaaatga taaaagtaag atgcttgatt tagaagggaa tattatagga accactaaaa
     3841 gtgaaatctt aaaaaatata aataaaaaat gtcaaggtga ttttttatct tgggataatt
     3901 ggagattata taatgtaaaa tatacgaaca taaagtggac taattggact ttagtatcaa
     3961 aatgtaatat atttaagaac tttaaaaatc taatagttaa ttttatgata aagttgtatt
     4021 tttatatagt aatatttatg atattgtgga aattaagtaa tgctaaatta atagaacaac
     4081 ttaaaaaatt ggcttattat gattctttaa gtggaataaa aaataaagaa aagtttagaa
     4141 aagattctat gtatatttta aagaattatt atcaagataa tttttactta gtacaattgg
     4201 atgtcaataa atttaaatat ataaatgaga tgtttggata tgctgaaggg aataagatat
     4261 taattcatat atcacaggtt ttaaataata atacaaataa atatgaaatt tgtgctagga
     4321 tggataatga ccattttata ttgttgattg cttgtaacac tgaagatgaa cttttaaata
     4381 gattatctaa aataaataaa gaaatttgta acttaaatac tactaactca tctaagtata
     4441 aaattgttat gtcaagtgga atatataaga ttactaaaaa ggatgatatt aaaaagattg
     4501 acttgttaat tgatagagca aatattgcag caaaaagtaa aaaggaaaaa tatgaacatt
     4561 catattcatt ttttaatgaa gatacaagaa atcgattgta caaggaaaaa cgattggaag
     4621 ataatatgaa taaagcttta gaaaaaggtg aatttatagt atattatcaa cctaagtata
     4681 gtttagatga tgtaaatgaa attgaaggag cagaagcctt gataagatgg aatagtccag
     4741 aatttgggtt tatttcacct atagattttg tacctttatt tgaaaagaat ggttttattg
     4801 taaatattga tatgttcgtg tttgaagaag tatgcaaaac attaaacaag tggataaata
     4861 aagggtatac tcctgtacca atctctgtaa atatgtctag agttcattta tatagggata
     4921 attttataga aaatataact gatttgataa gtaaatacaa tatatctcct gagtttattg
     4981 agcttgaact aacagaaagt gttgtgtttg ataatttaaa tatattgatt gatataatga
     5041 aaaagattaa agagatagga ttccttatat caatggatga ttttggttcg ggatattcat
     5101 ctttaaatct gttaaaagac ctttcttttg atattttaaa attagataga ggatttttaa
     5161 tcgaaacaac agatacaaaa cgtggaaaaa ttattatttc taagattgtg gaaatggcaa
     5221 aagcgattga tattaaagta atctgtgagg gcgtagaaac ttatgaacaa gtagagttct
     5281 tgaaagagat aggttgtgat aaggttcaag gatacttatt tgctaaacct atggtattag
     5341 atgaatttga aaaacactta aactttaaat ttgattaatc attaataatt attagactat
     5401 aagataccct aacaacttac ttacaaatat gtaagttaat tgttagggta tttttatggt
     5461 taaagtagta tattcacgat attatggtat tgtagtaaaa ataaaaaatt atggagaata
     5521 taaaatgaac atattgagta ttagaaatat atcaaaaaca tattgtggaa atattccatt
     5581 taaagcatta gataaggtta gtctaaatat agaaaaggga gaatttgtgt ctgttatggg
     5641 accatctgga agtggtaaat ctactctttt aaatataata tctacagttg atagacagag
     5701 tgaaggtgag gttgtattag atggatatga tgtaagtaaa ttgaaaggag aaaaacttgc
     5761 tgaatttaga aggaaacaac ttggttttgt atttcaggat tttaatttga ttgacacttt
     5821 aactgttggc gaaaatatta tgttgccact tacactagag ggggcaagta tcaaagatat
     5881 gaatatacaa actaaaagta tatctaaatt tttaggaata gataaaattt tagatagaaa
     5941 aacctatgaa atttcaggag gtcaagctca aaggtgtgct attgctagag ctattataaa
     6001 taaacctgcc atacttcttg cagatgagcc aactggaaat cttgattcaa aatctactga
     6061 tgatgttttg aaattattta ctaggataaa caaagaacaa aaggtaacaa cacttatggt
     6121 aactcatgaa gcatatagtg caagttattc agatagagta atttttataa aagatggttg
     6181 tatatataca gaaataaaaa aatctgaaag cagtaattct ttctattctg atatactagc
     6241 tgttttgtct caaataggag gtgtaagata gtggatttta gaaagtttgc ttataataat
     6301 atagttagaa atttcagggc atatttagca tattttttaa gttctgtttt ttctataatg
     6361 atattttttg tttttgcagt gtctatgttc catccaatta taacaaaatc tggaatgcag
     6421 agtggttcta ctgcatttat ggctcttatg acttcagaat taataatatt cggttttagt
     6481 ctacttttta tattgtactc tttaggaaat ttcttaaaat atagattgaa agattttgga
     6541 gtattgatga taattggaat gagtaacaga caactgaaaa aattgatatt aattgaaaat
     6601 ttgataatag gatttttagc agtgatgtta ggaatattac ttggtttatc aatttcaaaa
     6661 ttgtttttat tgtatttaag caaactgttt tatatgaatt taactgaatt ttattttcca
     6721 gtaaaagcaa tgctattgac tattgtatca tttatgattt tatttttgat aacaggacca
     6781 atgagcttaa aattattgag taaaaagagc attttagagc ttttagtagg tacaaaaaag
     6841 cctaaaaaag agataaaatc ttcaaagcta tttggaataa ttggtgtaat atgtcttata
     6901 ttaggatatg ctatgatact atttggcaat aagttaaaaa taaatggagg tatgtataca
     6961 acagcactat taattacttt ggggatattt ttattttttt ctcaatttac tattttgata
     7021 ttaaagtatt taaaaaataa agaaaaattt tataagaaca aagtaaatat gttacttata
     7081 agtaatttga tatataaatt aaaggataat acacgattgt tatttttaat aacaatactt
     7141 ttatcggcta ctttagtagc atttacaaca acttcaacac ttgtaagttc tcaaggagaa
     7201 gcttctaaaa ataatttccc aatggtttac tcatattttt cagagcataa taatgaaaaa
     7261 gaatatgaag aactacaaca aattagagaa gcaatcaaag gatatgatta taaggagtta
     7321 agttttcctg tacttaaata taatagggaa gtcttactaa gtgtaaagaa ttataacaaa
     7381 ttatcaaagg aattgggatt agataaaatt agttcaaagt tagaaaaata tgaagggatt
     7441 ataattccta gtagcaattc gcaagaacat ataaattctt taaaacaatt aaaaaattat
     7501 agtataaaaa atttaaatat agaagtaaaa aaatcaaaaa ataaaataat ttttcctaca
     7561 ggactgttta gtaaagttgt ggttataagt gatgacttat acaatgaagt aaaaggtgaa
     7621 ttttcaaaaa ttaattttca tggatttgat tataaaaact ggcaaagcag tgcaaagata
     7681 actgaaaatt taaaagaaaa acattttaat ttggatagag aatataacag gtcatatttt
     7741 ttaacattac cagatatgta tttagtagag ttacaacaaa gtaaaattct tcaatttatg
     7801 ggtgttttta taggagtgat attatttata gcagttttta gttttttata cttcagatta
     7861 tatacagacg agatagaaga taatgtaaag tacaaaaaac tatcaaaaat aggtctatct
     7921 tttaaaaata tgaataagat tgttagtata gagataggta cattgttttt tataccgtat
     7981 tttatagcaa ttataaatag cataatttct ttactgtttt taaatcaagt attaaataat
     8041 agttttaagt tagaaaattt gggtatatta ctactgatat catctatata ttcactttat
     8101 ttttacttat taaaaaaaaa ttatactaga aaagtttggt tttttgatta aagtggtata
     8161 taatagatat ataaattaat atttaaaaag tagttaatat taggggaggg catttgcacc
     8221 tcccttgtaa tatgtggaaa attgaatatt agaagaagga ggagacttga tggaaaatca
     8281 acaattatta ggaacagaac gaattagtaa attactacta aaatattcta taccagctat
     8341 aataggtatg ttggtaaata gtttatataa tgtagtagat agaattttta ttggaaatat
     8401 accaggggtt ggaccacttg caataacagg tcttggtgta acaatgccaa taatgacaat
     8461 aatacttgca tttgggatgt tgataggtat aggaactact actaccatat caataaaact
     8521 tgggcaaggt aaagtagaag aggcaagaaa acttatagga aatgcaatga cgttatctgt
     8581 aataacagga attatcataa tgatacttgg aatattattt gcaaataaaa tacttacatt
     8641 gtttggagca agtgaaaata ctttaatata tgcaaaatca tatatcaata ttatattatt
     8701 aggtacagta gtaaatcttc tttccttttc tttaaaccac tcaataagag ctgatggtag
     8761 tcctaaaatt tcagcaggaa taatgatagt aggatgttta actaatatag ttttagactg
     8821 gatattaata tttggattca atttgggaat acaaggagct gcaattgcta cagtaacttc
     8881 tcaggctctt acagctattt taactatagg gtattatata agtggaaaat caaacttacg
     8941 attcagtaaa tctaatctaa aattagataa aaagctaata aaagcagtgt ttgcaatagg
     9001 tatgtcgcca tttgctatgc aacttgcagc aagtttagtt caagtaatat caaatattgc
     9061 acttaaaact catggtggag atttagctat aggggctatg gctactattt cttctattgc
     9121 aatggtattt ttgatgccta tctttggaat aaatcaagga gcgcaaccta ttataggttt
     9181 caattatgga gctgaaaaat atgatagggt taaaaaagca tatttaggtt cattagtagt
     9241 agctactata attttatgta tgggtatggt agtagttatg cttttcccag aagctattat
     9301 aggtatattt aataaagacc ctgaacttat gaatatatct gttaatggat taagaatata
     9361 cttacttatg ttgccaattg taggcttatc agttacagga acaaacttta tacaatctat
     9421 aggaaaagca aaaatggcta tgttattgag ccttttaaga caagtaattt tattgatacc
     9481 agcagtatta attcttccta cattcttagg tttacagggt gtatggacag ctcaaccagt
     9541 gtctgatttt atagctacag taattacagg tatagttgta tttagagaat taaaaagata
     9601 tactcctaaa actgaaaaat taaatgaaaa tgaaagacta aacgagataa ctactgagta
     9661 aaaatataaa ttcagataat tgagcaagaa attgcaaaaa atacgattat atgataaaat
     9721 aaaaccgctg tcaaggataa gagaaattct aattatggag tgattttatg gaatttacaa
     9781 agaaaagcaa attaaaaaat atgtatgtaa acatgaatgt agctagaatg ttgtctaaga
     9841 taaatgagta taagggtaga caactgcttt acaaaaaaca accaaaagaa attttagaaa
     9901 acttagaaaa aaaatcttta gtagattgtt ctgaatctac atacattgga aatcaaagag
     9961 atagtagtaa ttttaatcta gaaaagttga taagtaatga agttacacct agaagtagag
    10021 aggaattgag tattgttgaa tacagagatg tggtaaaaac tattaacagt gcttatgaga
    10081 gtattcccat cagttcacaa acaattcttg agcttcatgg atacttatat aaattttcat
    10141 ctactagagg aggaagttat aagtcagata atgattttat tgaacacaat ttaaaaccta
    10201 gcgaatttat aagtactgat tcaagtgtca ataaagttga aaaagctgta gaagaaatat
    10261 gtgaagctta taatacattg atagaggaag atgaaattga tatattaata ttaatttctg
    10321 catttgtgtt agatttcata ttgatacatc cattcaaaga aggtaatata aaaatggcga
    10381 gagtacttat cctattgtta ctaaataaaa atgggtatga agttggaaga tatataagtt
    10441 taggaaagat atttgatgat agttcatatg aatattatag taatttaaat tatttgaaag
    10501 cctcaatagg tagtgaaaaa gcagatatga atgcatggat tgagtatttt ttagagacga
    10561 tattaactgc atatgagaag ctggatgata gtttaaatat atctgataag aaaagacaaa
    10621 caaaaactag tagaatagag aaaataataa attctaccct tggatatttt acaaaagaag
    10681 atataagaga tttatgtcct gacattccag aacctactat aaatagagtt tttaataatc
    10741 taagaaaaca agataagata gaagttgttg caagaggaag aagtgcaaaa tggaagaaaa
    10801 aatattaaaa aatgtttaga taactaaaat ggtatagtgt tttacactat accattttta
    10861 atgtcattag catgagcaat aatttaacaa cttaagcaat atcaatttta tttattgatt
    10921 atttttgaac tgaaaaatgg cgttatatgg tatacctcag tggtaattaa ttttggatga
    10981 gaagatataa tattacagaa tatattagga gtatatgtag aaaaaatata ataaaagtgt
    11041 gaggcttagt aattaacaaa ttatgtggct tcacactttt ttataaaata aagatactta
    11101 gcagaataat attttagttg atatgtttat aagatggtac ttgtttgggt aaaagtataa
    11161 atagtaaaaa agtagaagta atgtgtataa aaacataagt tacattctgt aataatagtt
    11221 attttataat tcaacaaaag aatagaatgg gtatatttgt gaaataataa tataaaataa
    11281 tcttaggagt gagcttatga agaaaaaatt attatatata aatgtaaatt caaaacctga
    11341 agacttatct tcaagtaaaa ctgtagcaag aaaatttata aataaattta tggaaaaaaa
    11401 taaagatttt gaggttgaag agatagacct ttataaagaa catataccaa gacttgagta
    11461 tcagtacttt gaaaaaagaa atagcatagt tagtgaagaa aatgctaaaa atttagacga
    11521 taaggataga aaagagctta caaagattag aaatctttgt gaccaatttg tgaatgcaag
    11581 tgtttatgta atagcagcac ctatgtggag tttatctttt ccagcaccac ttaaagagta
    11641 tatagattgt ataattcaag atggaaaaac tatatctttt gaaggcaatg ataaaccaca
    11701 aggaatttta aatgatattg atagaagtat ggtatatata caatcttctg gtggacacat
    11761 accatgggtg ttgaagccag ttatgaataa agggttaaat tatgtggaaa gtattatgaa
    11821 atttatagga attaaaaaat ttgatgaatt gttagtagat ggaactggaa ctagtgaaga
    11881 agaaagacaa gctgcaatag aaaaagcatc tgaaaaaata gatggtataa tagatgggat
    11941 gaaattttaa ttataatatt ttattaaaaa tataaaggaa aaacctcttt taatgtagaa
    12001 acttatattg aaggaggcga gaatcatgcg agaagaaaaa agtaatgaaa agtatgattg
    12061 ttattggtgt aatcaagaga ataatttctg tgtagaaata aaagataata tagtcatgat
    12121 agatgatggc actggtacgt taaaacaagc cgttttcata gggtataaac aaatccaaat
    12181 taatttaaac tgttcacatt gtcaaaactt aaatagaata aaattaaatt tgtagaattt
    12241 taaaatggaa attattgaag gaattaaaaa tagaaagtgt taatatttag actgaaaatt
    12301 atattttata caataaaagt ttatttaaat aacattgtat tttaatattt attttcagtc
    12361 ttactttatt aaaattattt tttggttatg tctggaaaat atctgtctac taatttttct
    12421 gaaatatttt ttaattggtc agccattgaa gtcagactta ataaggctat caataaatca
    12481 tctttatttt ctggatttat actaatattt tcaaaattag actctataaa gtccggtata
    12541 ttctttcttt gattttcttt tattaacatg aatttttcgt aacatgaaac taaatctttt
    12601 tcactgaaat tatccttttc atacacacca tgtaagattc ttttaatttc ttgtattgta
    12661 agtgtatttt tcatagaata aataattatc atctgtaaaa tttgttcttt agtatacttt
    12721 tttcccttaa ctggtttgat taatccctct ttgctgtaat tatttatcat ggttttagta
    12781 agaattttat cactttcaaa tcttttatta gcagaaagct tattgtcaat tagagaaata
    12841 atttgatcca tatataagtc aatagatgga atatcgtttg aagttatatc tgcattattt
    12901 agtgtttcta gtattatagt attaagttca ttattcatca tatgttacct ccataatata
    12961 tataatacac taaattagaa agtaaaacaa tattaaggct tgtaaaaaaa acataaagtg
    13021 gtataattaa atatataata tagtaatcaa aactatattt atgaatctga tttttaaaat
    13081 acataattgt aggaggacga aatgtatcaa ttttttttaa agggtagaga cccaataagc
    13141 agtttaactc attttatagg tgcttgttta tctcttttag caactataat tttagttttt
    13201 caatctgtta cattacagga aacttctctc ttgatgatag tatctgtatc tgtatttgga
    13261 ctttcactaa ttgctttata tagtgcaagt tcgtattatc attttttaaa ggggacacca
    13321 gaacaagaat tgttttttag aaaagtagac catgcaatga tttacgtatt gattgctggc
    13381 tcttatacac caatatgtct gaattttatg gaaaaaaaag aaggcataat atttgttaca
    13441 gcaatatgga tagttgcatt tattggcata ataataaaaa tattttggat ggatgcacct
    13501 agatggttat ctaccagtat atatttactt atggggtggg caattgtttt tgatataaat
    13561 gcattcaatt caattcctaa ggattgcctt agactattaa ttatggaagg tgtatcttac
    13621 tctataggtg cgattattta tataattaaa aagcctaata taagtcctga gtttggattt
    13681 catgaaatat ttcatatttt tattatgata ggttctttat ttcacttttt agcagtttta
    13741 ttgtatgttt tatagtttta aaagatttga ttaattgata ctaaaattct acaatgaata
    13801 aaatggaact gccttatata gattatatct tagacaataa gacagttcct tattataaat
    13861 attaaattct acttataata aaattttgat aaaaatagtc cattcaaaat gcctattcca
    13921 taattccttt aatgtacttt tcatttacga catatagttt gaatttatgt actataatta
    13981 tatcctctaa attctctgaa acactaacaa ttctgacaca tttgtttcta atttctacag
    14041 gtaaatctaa atcatttgtt aaattcacat agtcatcata tttatacata aaatcatccc
    14101 tttacattaa gatatactta ttatactata ttttaataag tttttttaaa ttattacaat
    14161 ttttcccgaa atttcatttt ttatacataa aatttccaaa ttaggtaa
//
LOCUS       DAEOSP010000099        14066 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.099, whole genome shotgun sequence.
ACCESSION   DAEOSP010000099 DAEOSP010000000
VERSION     DAEOSP010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14066
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.099"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            153..1304
                     /locus_tag="KRH54_002225"
     CDS             153..1304
                     /locus_tag="KRH54_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8579882.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLESLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            1422..2060
                     /locus_tag="KRH54_002226"
     CDS             1422..2060
                     /locus_tag="KRH54_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBG8579883.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            2061..3083
                     /locus_tag="KRH54_002227"
     CDS             2061..3083
                     /locus_tag="KRH54_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBG8579884.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLDTLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            3073..3693
                     /locus_tag="KRH54_002228"
     CDS             3073..3693
                     /locus_tag="KRH54_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579885.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDFKMNDYMENCNM
                     MAGLALDKVGIVCLDCINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(3817..4503)
                     /locus_tag="KRH54_002229"
     CDS             complement(3817..4503)
                     /locus_tag="KRH54_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBG8579886.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKEYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDILGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(4517..5062)
                     /locus_tag="KRH54_002230"
     CDS             complement(4517..5062)
                     /locus_tag="KRH54_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8579887.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            5290..6537
                     /locus_tag="KRH54_002231"
     CDS             5290..6537
                     /locus_tag="KRH54_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBG8579888.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            6624..8618
                     /locus_tag="KRH54_002232"
     CDS             6624..8618
                     /locus_tag="KRH54_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG8579889.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNYCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            8618..9940
                     /locus_tag="KRH54_002233"
     CDS             8618..9940
                     /locus_tag="KRH54_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBG8579890.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNLEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            10219..11505
                     /locus_tag="KRH54_002234"
     CDS             10219..11505
                     /locus_tag="KRH54_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG8579891.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            11591..12898
                     /gene="grdB"
                     /locus_tag="KRH54_002235"
     CDS             11591..12898
                     /gene="grdB"
                     /locus_tag="KRH54_002235"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:12638..12640,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG8579892.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            13176..13907
                     /locus_tag="KRH54_002236"
     CDS             13176..13907
                     /locus_tag="KRH54_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8579893.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
BASE COUNT         5634 a         1401 c         2448 g         4583 t
ORIGIN      
        1 actataaatt aggtttatat tttataaaaa tttgctaaaa aaaacaatta aaaattgtaa
       61 agaaaaaaga tataattaga actgtagtaa taaatgtttt attatagata atagagtttt
      121 aagtatctta aataatattt ggaggaatat gaatggaatt aagaccaaga agtgcaaaat
      181 tagaagaatt tgataaagta atagaattaa taaactatgt atttagaata tcaagaaatc
      241 ataaaccaac tatgatggaa gaatttcctc ttttactaag taaaaataat atagaaaata
      301 tgattataat atcagaagat gataaagtgg tttctgatgt aaactatcta attcaggatg
      361 tatctattca aggaaacaga ttgaaggttg cagctatagg tggagtatgt actcatccag
      421 attatgaaaa gagaggctat tcatctaaga tattggataa agtagaagaa aaaatgtttt
      481 atgatggtgt agatattgta attatatcag gaacaagaag tctatacagt agaagaaatt
      541 gtagtttggt aaagagtttt tataaatata caataaaacc tgaagatgta aaaatagcat
      601 atgaaatagt tgaatttgat gaaactaatt ttgaaaaaga taatgattta gataaaatga
      661 ttgaactata taatcaaaat agtactagat ttataagaac gagagatgag tttcaaaaac
      721 tattgcatgc agcaactatt gcctggggac caattggtta caagaaggta tttataaagg
      781 aaaataataa tataatagga tatttgataa taagaacaat aaaaaaagaa gattctactg
      841 taggagaagt tgctgaaata gggttaaact ctgtaaatgt tgaaaatata ttgaaatatg
      901 ttgcaaataa atttggatta gaatctttaa attataaagt acatgtgaaa aatcttaaag
      961 accagttgaa atgtaatggg acaaaatctt tggattatca acaaggaact atgaaaataa
     1021 taaattttac taagttgtgt gatagtttaa gaagttattt tagtcaatat gtagattttg
     1081 aattactaaa atacatggaa tttaaacaag ttgaaaataa atatattatt aaatacaaag
     1141 aagaagaact tgtaatagaa aatttggata agcttaataa actgtttttt gaaaaaaatg
     1201 aggaacagta caatgaattt aaacatctaa aaaatatata tgaatttgct acaaaggcat
     1261 ttcctgtaga ttttccatgg actgcaaatt taaattatca atagttttag atgatattaa
     1321 ataaaattga ttgataccta gagttagtcc ttgttaaata agagaaatta ttggatattg
     1381 aaaaagcaat tttgtaagta attagaaagg atgagtatta tatggacata cttgatgata
     1441 tagcagaatg tatattaaat atgggtgtag ataatataga gaatttagta aaaattgcta
     1501 tagataaaaa tattgatgtg gaagatattt atgaatatgg tttaaacaaa ggtatgatag
     1561 gagctttaga taagtttgag aataaggaat actatctttc agaagttata gtatgcacag
     1621 atgcattaaa taaaggtatt aatcttttga agggaactgg aaaagtaaag aaaaaatcaa
     1681 agggagtaat tctgatgtct gtagtagaag gagatactca tgagatagga aaaaatattg
     1741 taaaagtaat ggtagaagcc actggatata aggttattga cttaggtgta aatagaaaaa
     1801 gtgaagatat aatagaagaa gctataaaaa acaatgtaga tataataggc ttatcttcaa
     1861 tgatgacaac tacaatggaa aatatgaaat cggttataga cgaacttaat atgattgaga
     1921 tagataaaag accaaaagta ataattggtg gaggacctgt aagtatggag tttgcagaag
     1981 aaataggagc agatggatac agttcaaatg ctccaaaagc agttaaatta ataaataaac
     2041 ttataggagg tgaagtttaa atgttttcag atatgacttt acttcagtat ataaatagtg
     2101 aagatagagg tttttttctg cctgatatgg gtacaaatgg attgttctta ataaataaaa
     2161 aggcatatga agtatatgat agtgtagatt tgcaattaga attagcaaag actatggaca
     2221 agtattttga atcagatttt atatactcat tctgtgatgg gattacattt tgtgaaactc
     2281 tgggtttaga tacattaaga ccagattatg attttccaag tgtattgaca catactataa
     2341 ctgacagaga taaattaaga aaattagatg ttccagaccc ttatacgagt ggacgtatgc
     2401 cactcaatat agagagctta tcagttatat caaaaagtat taaaaaacca ctatatgaat
     2461 cgatacaagg tccatttaca cttgcaggtc aattagctgg agctactcaa cttttaagat
     2521 gtataataac tgataaaaaa tttgtagaag aattattgga atttacaact gaattggtaa
     2581 gaagatatgc tgtagctgca aataaagcag gagcaaaata tatttcaata gcagagccaa
     2641 catcagtaac cctaagtaaa gatagatttg atgaatttat agtaaaaaat ttaaataaaa
     2701 tctacaatga acttgattgt tggaaaggta tgcatatatg tggagacacc agagaattat
     2761 tagataatat gctaagttgt aatatagatg cagtaagttt agaccaaatt ttagactatg
     2821 aagaaattgc tccaatgatt cctagagata tagttttgat tggtaattta gacccaataa
     2881 aacttcttgg acgttctaag ccagataaga ttagaagaga gacactaaaa ttgttaaaga
     2941 agatgagaag atatgataac tttttatgtg attttggctg taactgctta aataccactc
     3001 cagtagaaaa tttacaagca gctataaagg caggaagaat tagttataaa gaattagata
     3061 ggattgatat tgatgaatta taatagtaat aaaaataaat atcttttaaa ggatataaat
     3121 ttagatgata tttttataga taagaatatt gtgctaaagt ttttggggta tggaaataga
     3181 aaagctccag atacaattag taaaatcgtt gaaagagaaa taagaaatat aaatgatatt
     3241 ttagatatca agatatatat aagtgaagtt gatattaata aaacacctaa agaatgtaaa
     3301 aaagcatttg ctgtactata tacaataggg gataaaattg attttaagat gaatgactat
     3361 atggaaaatt gcaatatgat ggcagggctg gcattggata aggtaggtat agtatgttta
     3421 gattgtatta atgagaaaat aaaaatgtat ttaaaaggaa agtatgatag tctcaaaata
     3481 tcgcatgaaa tatatccagg agataaagat tttgaagtag agagacaaaa ggatatttat
     3541 aactatgtaa aaaataaata taatagtata gaaatagaaa ttaatgatta tcatcaacta
     3601 tcccccataa aaagtgttgc aatgcttatt ttaatggggg atgaagaaaa tttagaatca
     3661 agatgtagta agtgtcctag aaaatgcttt tagttaattt tcaagactaa gatttgtttt
     3721 gtgtataaat tgcaataatt tttttggtca ttttatgtat aaactaaaaa aattattgca
     3781 aagtaatatt ctatttaatt tgaacagact tacaaactat agtagtttct ttaattcaat
     3841 aagtgtattt atttcataag ttggtatatg attagtataa tttatactgt tattggaatt
     3901 taaccaacaa gtatctattc ctgaatttat tccacctaag atatcagagg ttaagctatc
     3961 cccaattatt aaggcagaag atttatcatg acaattaaac tttttgagag cgtatttaaa
     4021 tatttcagga tttggttttg atattttcaa ctcttcagat acaaccattc catcaatata
     4081 ttcttttatt tttgaatttt caagtctttt cttttgaact gcagaaattc catttgttat
     4141 tataacaata gtatactttt ttttaagata ttgtaatata tcgattgcac ctggaattaa
     4201 aaatgtacat tcacctaaaa aatttaagta cattttgcta agagtttcag aatcaacatc
     4261 caaagatatt ttatttgcaa aaagttcaaa tctaagtaat ttaagttcat tcaaagttat
     4321 aatatttttt tcaaggtcga gccaaagctt atcacttata tttctatatg actctatata
     4381 attctcaaaa ttaaattcta aatcaaactc agatagaagt tttttaaaag catgtgattc
     4441 acttttctta aaatcaaata aagtatcatc tgcatcaaaa aatataaatt tatatgtctt
     4501 catggttttt actcctttac aagatgactg caaaaattac tttactttca ctatttgagt
     4561 cattttgcca tttatgtttt gttcctaaag gaacttttac actatctcct ttggacaaag
     4621 taaattcatc atcatccata aaaagtttaa cttctccctc caaaacatat gcaatttcat
     4681 ctccattatg agtaatccta tcaacacaag aagaagtgtt tggagctaaa tccataattg
     4741 caaattctaa attaccacta gagtctggaa ttaaaatctc atatataact tctttgcttc
     4801 caggtaaaat aacttttttt ctattatcat gtcttactat caagtttttt ttatctacct
     4861 cagatacaaa aaatgtaaat aaaggaacgt ttagagaaga ggctatagat tttaatgaat
     4921 ttacagatgg attagcaaga cctcgttcta tttggctcaa taatgatggt gtaacatcag
     4981 ctagttttgc taaatctcga atacttaagt tttgtttttt tcgaacttca gctatttttt
     5041 cccctaaatt taaatcattc atataaatat cctctctttt taatttaaaa aatattaact
     5101 taaaataatc aagtaaaatg attatgtagt atgaaattat tataacatat agaattaggt
     5161 tttgaaaaaa ctatgttcaa tataattaaa taaattaaaa tataattgac aaagtggata
     5221 cagaatactt aaaatatatt taaataaaat taagcaaaag atagttaatt tatgatagga
     5281 ggggatatta tgcaaataac gacttttgga gctatatttg gtttattaat agcaattatt
     5341 ttaattataa aaaaatttca agctgtatat agtttaatgc taggagcatt tattggagga
     5401 ttagttggag gagcaaatat aacacaaact gtagatttta tggcaaatgg agctatgaat
     5461 atatcaccat caattcttag agctttggca tcaggtgtat tagctggaag tttaataaaa
     5521 acaggggcag tagataaaat atcagaacag attgtaaaaa tatttggtga aaagagggct
     5581 ttattttcga ttgccatttc aactatggta cttgctggag taggcgtaaa tttggatgta
     5641 tctataataa ctgtagcacc tataggatta tatataggta gaaaattaaa ttattctaaa
     5701 ttgtcaatac tattagctat gttgggaggg ggtaaagcag gtaatataat atcaccaaat
     5761 ccaaatacta tagcagtagc agataatttt tctgtcaatc tctctagtgt tatgatggca
     5821 aatataattc cagcaattat tggagttgtt attacagtta ttttagcaag tatattaata
     5881 aataaaggaa ataaagtaca atcatatgaa attttagagc aaagagaaga tttaccaagt
     5941 ttatttaaat cattatgtgg tccaattata gctatatttc tattgttttt aggaaatgta
     6001 tctcctatag ttatagaccc aatgatagcc cttccaatag gaggaatcgt aactctaatt
     6061 gtcacaggaa acttgaacaa ttcaagagaa tatttagctt ttggattatc taagatgcaa
     6121 ggggtatgca tattattact tggaacaggg actatcgcag gaattataca gatgtctgaa
     6181 ttacaacaga gtacaatagg tgctttacaa tttttaaata tgccacaatt tttattggca
     6241 ccagtttcgg gtatacttat gtcattagca acagcatcat caacagctgg tgctactata
     6301 gcatcatcta cttttcatga tgctataata aatggtgggc tatcaccgat ttcaggtgcc
     6361 tcaatagtaa atgctgggtc tagtgtattc gagcaattac cacatggctc actatttcat
     6421 actagtgcag gtagtataaa catggatata ggtgaaagat ttaagttaat tccatatgaa
     6481 gcattgatag gaatagtaat gactataatt tcaacatcta ttcaattagt tttataataa
     6541 atataaatat ttaatgaaaa aagtaggatt ttgttattaa atattgaatt tattatagta
     6601 aaaaagaaga ttggagtttt aaagtgaata aaaaaaagat tgttattata gggattattt
     6661 attcattttt agtagtattt tcacttacaa atatgtatgt aaatatggag tataatctaa
     6721 atgtatttga atatattaag aagtcacttc catttacaga agaagaaaaa aagtggttag
     6781 aaaaacacaa gaatttgatt tacagctctg accaaagttc acctcctttg agatataaag
     6841 gtaaggaaga tggacagtat aagggaatta ttgttgattt aataaattct ctgtcaattc
     6901 aaattggtag agatttttat tttaaaccaa ataattggtg gaaagaatct ttcgtaaatt
     6961 caatagatga tagtataaag ttttttgact tgattccatc aaaagaaagg gcaaataaat
     7021 ttatatttac tgacccaata tacactttga gtgcaaatat attgaaagat aaaaagtccc
     7081 aagatataaa tagttacatg gatttaaagg ggaaaacagt agcaattcca gaaggtgatt
     7141 attctataaa ttttttaaag caaaaaattc aagatataaa cattttgttg actccagata
     7201 taaagactgg agtcaaccat ttaatgtctg gaaaagtaga cgcagttgtt ggtgatgaac
     7261 cagtattgcg atactatata aataattatg gactttcaaa taaatacagt gttttaagta
     7321 atcctatata tactaaaaaa gcagttcttg ctgtacctaa acaatatgaa gagctggtta
     7381 gtattttaaa taaaggcatt tttaaacttc agaaaaatgg agtgtataaa gatttaaaga
     7441 aaaaatggta ttctacgtat aatgaagttg atgatatatt gtatgagcga ggaattgtgc
     7501 ctagtattta tctttttata ggtataattt taatatcaat ttatgttttt tatagctata
     7561 catatttgtt aaagatagaa atcaaaagga aaacagaaca ggtaatagaa aataaaaaaa
     7621 ctttagaggc aacttttaat agtattacag atataataat gcttgtagat gaaaataaca
     7681 atatagtaga atcgaacaaa gtactatatg actttatggg tgaaatgagt tataagatag
     7741 cagatttgat tagcatgata aaaggtgtta ttgaaaatac cttctctgaa aatactaata
     7801 aaactagtga aatagaaata cacaataaaa ttttaaagat aaacactttt cctgtagagt
     7861 ataaaaaaaa taatactgag tatatagtag ttcttattaa agatataact aatgataaaa
     7921 tagtagaggc gaaattactt agagaaaaca aaatgatatc tataggacaa ttagcttctg
     7981 gggtagcaca tgagataaga aatcctttgg gtataataag aaataattgc tatctcttaa
     8041 aagataacgt tacaatggaa gaagtaaatg attgtgttaa atcaatagaa agtaatgtag
     8101 atagggcaag taatataata accaacttgt taaatttcgc aagaatatca gatgataatt
     8161 tagaacatat aaatataaga aattttattg aaaatatagt gaaacttcaa tataaaatgt
     8221 tacagttaaa gaatgttgaa ataaaaattg actgtgaaca taatttagta tgctatataa
     8281 atggagaatc gttaaaacat gtatttataa atttaatttc taattcaata gatgcaatac
     8341 atcaagatgg aaaaattatt atatactgtt atgaaaaaaa ttattgttta tttatagatt
     8401 ttaaagataa tggtgaagga ataaaagaag atgccctaaa ggacatattt aatcccttct
     8461 atacaacaaa accaattggt gagggaactg gtttaggttt atacattact tataatgaga
     8521 ttaaaaagaa taatggagat ataagtgttg aaagtaaatt gggggttgga acttgttttc
     8581 atataaaaat tccattaaat aaagaggtga ctatataatg aaaatactat tagttgatga
     8641 tgagctagaa tatggagtgg taatgaaaaa aattctacaa aagaagggct atcttgtaga
     8701 tatagcacta agtggtgaag aagctataaa tataattaaa aaagataaaa attatgattt
     8761 ggttttgtct gatgttatga tgaaaaatat ggatggagta cagctattag atagaattaa
     8821 atctattaat agagatatag aggttatatt agttacagga tatggaagta ttgaaaatgc
     8881 agttgatgct atgaaaagag gagctttatc ttattttata aaaagtaatc ccattgaaaa
     8941 tttattagaa gaagttgaaa aggtaaaggc aagtaaaata tctgtttctg tacaaaaaaa
     9001 taatctggaa tttactttgg aatctaaaaa tagagatttt aataatatca taaaaatagc
     9061 aaaaaaagct gcttgtaaag atgttaatat attaatcttg ggggaatctg gtgtaggaaa
     9121 agatatatta gctagatata tacatagcat aagtccaaga aagaatgaga tatttgtacc
     9181 tgtaaattgt tgttcttttt cagaaaatct attggagtct gagttgtttg gtcatgaaaa
     9241 aggttctttt acaggagcag tagatagtag aaaaggtaga tttgaacttt cgaataaagg
     9301 aactctattt ttggatgaaa taggagatat acctctaaat gttcaggtaa aacttttgag
     9361 gacacttgag gacaaatcta tagagaggat aggaagtaat aaatctatta aagttgactt
     9421 taggttaatt tgtgctatga ataaagaacc aaaagtggaa atatcaaatg gaaatataag
     9481 agaagatttt ttttatagaa taagtacaat aacaataact ataccaccgc taagaaagag
     9541 aagagaagac ttaactactc ttatagaatt tttcttaaat aagtaccaaa ttgaacatga
     9601 taaaaagata cactcgattg ataaggaagt aaaggatttt ttattaaatt acaattatcc
     9661 aggaaatata agagaattaa aaaatataat aaatagactt gtagttttaa gtgaagaagg
     9721 caatttatca aaagacaatt taaatttaat atcaaataat gtatacatag atgataaaat
     9781 aagtattaga cctttaagag aaataagaaa agagtttgaa tgtgagtata ttgaaaaagt
     9841 tttatctttg tgtggaaata atatttcaaa tacagctaaa aaattggaga ttagtagaag
     9901 acagttaacc aataaaatat cagagtataa tattaagtga gaaaaaaact tctcattaag
     9961 tgagaaaaaa atttctcaaa ctgtattaaa agataaacag aaaaaatctg tttatctttt
    10021 tttattataa ttttaaaaat taatatttac aataattatt gctaaattca atattggtgt
    10081 aaattaaaaa tgaaaatttc tatgacatta atacatagaa tatatatttt aaataattgg
    10141 catagaaaat gctttatata ttaataaacg taataaatac ttttaattgc gattaaatat
    10201 attaaggggg attttcatat gaagctagag ctagggaata tatttataaa aaatgttgaa
    10261 tttggagaaa agacagaagt aaaagatgga gtactatata ttagtagcga agaaatagaa
    10321 aagatagtat tagaagatga aaggattgta agtgtaaatg ttgaacttgc aagacctggc
    10381 gaatcaataa gaatagctcc agttaaagat gtaatagagc ctagagttaa aattggagat
    10441 gaaagcaaaa tatttcctgg aattataaat aaagtgaaga ctgtaggaag tggaagaacc
    10501 catgtacttt taggagcttg tgttgtaact tgtggaaata tagttggatt tcaagaaggc
    10561 gtaattgata tgagtggtcc tactgctaaa tatacacctt tttcaaaaac aaataatata
    10621 tgtattgtta taaaagcaaa ggatggtata gatactcatg attatgaaga agcagctaga
    10681 attgcaggat taaaaatagg tgcatatgta ggtgaggcag gaagagaagt tgagccagat
    10741 gaagtcattg tatatgagac aaagccatta ttaaaacaag taaaagaata tccagacctg
    10801 ccaacagtag catatgttca catgttacaa tcacaaggac ttttacatga cacttattac
    10861 tatggggtgg atgctaaaca gatagtgcct acatttatgt atccaactga aataatggat
    10921 ggagctataa tatctggaaa ctgtgtagca ccatgtgata aggttacaac attccatcat
    10981 ttaaataatc cagttataca tgacttatat aaaagacatg gaaaagattt aaactttata
    11041 ggagttattt tgactaatga gaacgtattc ttagtcgata aagagagaag ctctgatatg
    11101 gtagctaaat taatagaatt tttaggagtt gatggagttc tagtgacaga agaaggttat
    11161 gggaatccag atactgacct aatgatgaat tgtagaaaat gtagtgaagt tggagcaaat
    11221 gtagttttga taacggatga atttcctgga aaagatggca agagtcaatc tatagcagac
    11281 gctactaagg aagcagatgc agttgtttct tgtggtcaag gaaatctagt ggttcatttc
    11341 ccagcaatgg aaaaaattat aggaacttta gattatgttg aaatgatgat aggtgggtat
    11401 aaaggatgct taaatgaaga cggaagcatg gatgcagagc tacaaattat aatagcatca
    11461 actatagcaa atggctataa tcatttgaca gcaagatatt attaaataag tatttgaata
    11521 taaagttctg ttgatataat cattggtcta attgatgtaa caactataaa gtgatttgga
    11581 ggattaagag atgagtaaaa tcagagtggt tcattatata aacaatttct ttgctggtat
    11641 aggtggagag gaaaaagcag atattccgcc agaaaaaagg gctggtgtag taggacctgg
    11701 aatagcattt cagagtcaat ttaatgatga tgcagagata atctcaactg taatttgcgg
    11761 agatacgtat tttggtgaaa atattgagag tgcaactcaa gaacttttaa aaatgataaa
    11821 agaagaaagc cctgatttat ttattgcagg cccagcattt aatgcaggta gatatggagt
    11881 agcttgtggg actatttgta aggcagttga agaagagtta aatattcctg taattacagg
    11941 catgtataaa gaaaatccag gtgtagatat gtttaaatta gatttgcata tagtgtctac
    12001 aggaaattca gcagcaagtc ttagaaaggt agttccaata atgacaaaac taggtcttaa
    12061 attggttaga ggtgaggaaa taggacctcc tgaagaggaa ggatatataa tgagaggaat
    12121 aagaaaaaat ttcttccatg aacttagagg gtctgaaaga gcaattgata tgttggttaa
    12181 aaagatgaaa ggggaaagct ttgaaactga gtatccaatg cctgaatttg atagagttac
    12241 acctatgaaa cctgttaaag atttatctaa gataaaactt gccctagtaa cttctggagg
    12301 gatagttcca attgataatc cagacaaaat tgaatcttca agtgctacta aatatggaat
    12361 ttacgattta acagatatgg attctatgtc aaataaagaa tttacaacta tacatggggg
    12421 gtatgacaga gcatatgtcc ttgaaaatcc aaatttagtt gtaccactag atgtagttag
    12481 agagcttgaa aaagaaggtg taattggaga attagtagat tattttataa ctactacagg
    12541 aacaggaacg agtgttggca attctaagag atttggagaa gagttttcta aaaaattgtt
    12601 agaagatgat gtagatgctg taattttaac ttctacatga ggtacttgta ctcgttgtgg
    12661 agcaacgatg gtaaaagaga ttgagagagc tggtatagca gttgttcata tatgtacagt
    12721 agtaccaata tcacttacaa taggagctaa tagaattgtt ccagcagtag ggataccata
    12781 tccacttgga aatcctatgt taggagaaga ggaaagtaaa aaaattagaa agaatatagt
    12841 tttaaaagct ttaaatgcac taacagaaga tatagaagaa caaacagtat ttgaataata
    12901 aaaatacaac acaacactga aataaaaaaa gatatcaaaa aataattttt taaaattatt
    12961 ttacgatatc ttttttttat taggtaaatt cgaaatttgc attttttaaa attaaattta
    13021 taaaagtgat aaattagttt attataatat tgatacacaa attcaaatta gatatactat
    13081 gaaaagcgag attattacat tttggaacaa atattcaaca tattagatca tattaattga
    13141 acagaaaaaa ttaactttat ataataatta gaataatgat aagaaagaag gtgtatatat
    13201 tgatttcaat tgccatttgt gatgatgaat cctatatgct aaatcattta aaggaaaata
    13261 tagaattata catgaagcag aagaatttat tatataatat agagcttttt aataaagtag
    13321 aagatttact atgcaatcat gaaaagttta atattgtatt tttagatata cagatggaag
    13381 agattaacgg aatgcaggca gctagaaaat taaggtcata tggtaatgag tgctttataa
    13441 ttttcgttac agttttaaat gaatttgtat ttgatgcttt tgaagtagat gctataaatt
    13501 atctgataaa acctctatct aatgagaaac tatataaaac tttggataaa atagttacaa
    13561 aacttgatga taatgaaagt aattatatca caatacaaaa aggttattca tattggaaat
    13621 taaaacttgc tgatatttta tattgtgaag ttataaaaag aaagatttat gttcatttaa
    13681 agaaagagac tgtagattat tatgagacta ttgaaaattt agagagaagt ttaccaacaa
    13741 atttttttag gtgtcatcgt agttatataa tcaatttaca atatgtatat ggatatgaaa
    13801 atggtaatgc agttatggaa agtggagata gaatacctgt ttctagacta cgtcaacaaa
    13861 tattttcaaa agttatgctt gagttcatga aaaataggag gatataagat gattaattta
    13921 ttttcttccg atttattttt aaagtacagt tttttatttt cttttattgt atatagaatt
    13981 ttaatggtaa tagtgcaata ctattttcta atcagtttta caaatgttaa ttcaaaattt
    14041 tggtgtttta ttttatattc gataat
//
LOCUS       DAEOSP010000100        14024 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.100, whole genome shotgun sequence.
ACCESSION   DAEOSP010000100 DAEOSP010000000
VERSION     DAEOSP010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14024
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.100"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            444..1514
                     /locus_tag="KRH54_002237"
     CDS             444..1514
                     /locus_tag="KRH54_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8579894.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1498..2367
                     /locus_tag="KRH54_002238"
     CDS             1498..2367
                     /locus_tag="KRH54_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBG8579895.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2411..3271
                     /locus_tag="KRH54_002239"
     CDS             2411..3271
                     /locus_tag="KRH54_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBG8579896.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3302..4606
                     /locus_tag="KRH54_002240"
     CDS             3302..4606
                     /locus_tag="KRH54_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG8579897.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4669..5931
                     /locus_tag="KRH54_002241"
     CDS             4669..5931
                     /locus_tag="KRH54_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBG8579898.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEIVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6118..8088
                     /gene="uvrB"
                     /locus_tag="KRH54_002242"
     CDS             6118..8088
                     /gene="uvrB"
                     /locus_tag="KRH54_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBG8579899.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     IDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8107..10932
                     /gene="uvrA"
                     /locus_tag="KRH54_002243"
     CDS             8107..10932
                     /gene="uvrA"
                     /locus_tag="KRH54_002243"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG8579900.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11191..13008
                     /gene="uvrC"
                     /locus_tag="KRH54_002244"
     CDS             11191..13008
                     /gene="uvrC"
                     /locus_tag="KRH54_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBG8579901.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13008..13937
                     /locus_tag="KRH54_002245"
     CDS             13008..13937
                     /locus_tag="KRH54_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBG8579902.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
BASE COUNT         5706 a         1613 c         2546 g         4159 t
ORIGIN      
        1 ggtaaattta taaaaattaa aaaagtttta ttttttatgt ttaaaccttg taaatttggg
       61 acataatata attgtaaaac ttaatatagc aaaaataaat tactcaatct cccccactta
      121 aagacctcta tcccccatag aggtcttttt tattttcaaa ccaaattata taaaaaataa
      181 atagttttat atgactcttc ataaattaaa atcacattgt gatagcaaac catgaatatg
      241 aaaaatatag aaaattatag ctaacataag ctgtatatca tcttaacttc agtatattta
      301 acaaaaaatg tttataattt aacctttatt ttaataagtt tactatatat tcactttgtc
      361 ttaatatatc ctttacaaat taagagtatt attaatctat aagtaaatcg tagaattaaa
      421 ttaaatattt tgggaggata aaaatggcag atataagact agaaaatgta acaaaatctt
      481 ataataaaaa aaatacagtt atagaaaatt taaatcttac tatagaagat ggttctttta
      541 cagtattggt aggcccaagt ggatgtggta aatctactac gttaagaatg attgcaggtc
      601 ttgaagatat aacttcaggt aggttgaaga ttgaagaaaa tgatgttaca aaaacggatg
      661 ctagtaaaag ggatatagct atggtatttc aaaattatgc attatatcca catatgactg
      721 ttagagaaaa catagaattt ggtcttaaaa acaaaaaagt agaaaaagaa aaaagagaaa
      781 aacttataac agaagttatt gatgtagtag gattgagaga atatttaaat gtgaaaccag
      841 gcaacttatc tggaggtcaa agacaaagag tggcacttgc tagagctatg gttaaaaatc
      901 caaaggtatt tttaatggat gaaccattgt caaatttaga tgctaaatta agaaatcaaa
      961 tgagaacaga attaatacaa ttacatcaaa aactaaagtc tacatttgtt tatgtaactc
     1021 atgaccaggt tgaggcaatg tcgatggcag ataagatagt tataatgaac aaaggaaaga
     1081 taatgcaaat aggttctcca aagaagatat atcaaaatcc taaaaattta tttgtagctc
     1141 aatttatagg gagtccttgt atgaacatac tagatataaa aaatgatatt aaatttggat
     1201 tcagaccaga aaaagcaaca ctagagagta taaacataga tgggaaaaat gatgatgtat
     1261 ttagtctatt tggaaaagtt gtaactaaag aagtgttggg aaatgaaact atatattgtt
     1321 tatctgttct taatagagag ataagagtca aaacggaaaa tgatatattt gatattggaa
     1381 gaactttaag aataagtgta ttagaaaaag atttatatta ttttgataaa aatggagaaa
     1441 gaatagaaga tgtaaaagta tgtaaaagta tgtattctaa aattaaagga gatatttatg
     1501 aaaaagcaat ttaataaagc atatttatat ataacacctg ctattatagg aactttattg
     1561 tttatagtct atccaataat taagacgata tctctaagct tcaaatctgg tagtcttctt
     1621 agttcaacct ttgaaaatgt aggcttaaac aattatacag aacttttaaa gaatccagag
     1681 tttatacaaa ctataacgaa cactgcaatt tatacttttt ttatggtgac tatatctata
     1741 tcactagcca tagtacttgc tgtttggcta aataaaaatt ctataataca caatttaacg
     1801 caaagtataa tctttacacc acatgtaatt tcactagtat ctgtagctgt attgtggctt
     1861 tggattatgg agccacaata tggcttttta aactggatac tatcgacatt acatttacca
     1921 acatcaaagt ggctttcaag tgaaagttca gctttaatgt cactaattct aataggaata
     1981 tggaagactc ttggatataa tatcttaata gtgttatcag gtcttaaatc cataccaaac
     2041 tatatatatg aagcatctaa attagataat gcaaacaaaa taacgaattt ctttaaagtg
     2101 acattgccac tattatcacc aacaatattt tttcttatga taacaacaac aactgcatct
     2161 tttcaagtat ttgatgagat aagtataatg acacaaggtg ggccagtggg ttcaactaac
     2221 atgttatctt attatatata tgagtctgga tttatatact atgacatagg acaagcgagt
     2281 gcagcgagta tgtttttgtt agctatagtt atgattgcta catatgctaa ctttagactc
     2341 ttatcaaaga aagttcatta tcaataaaga aaaagtagat attataaggt ttatccaaaa
     2401 ggaggagaca atggatatta tacaacctat caaaaaagac aaagttgata taaaaaaaat
     2461 agcatcaaaa tcaatagaaa tattaattct attactttta tctgcaatat ttatattccc
     2521 ttttatatgg atgctatcta catcctttaa aaatccacaa gaaatgatgc aattaccgcc
     2581 cactattata ccaaatgata tagtgttaga aaattatagt acagcatggc attcaggtcc
     2641 attttttaga tatttgctaa acagtgtatt tgtaacagga agtgttttaa ttgtacaatt
     2701 tttagttatg gttccagcag catatgcatt ttctaagatt aaatttaaag gtgaaaaaat
     2761 gctatttggg cttttgcttg taggacttat gattccacca caagtaactt ttctaccagt
     2821 ctatatgatg atgagtaagt ttggatttat aaatacttat gttccactta taattccatt
     2881 tatgacatct tcatttggga tatttctgct tagacagaat tttatgcaga tttcagatga
     2941 aattattgaa gcagctaaac tagataatgc ttctgactta aaaataatgt ttagaataat
     3001 ggttcctatg gcaaaaccag cagtaataac atttatatta tttaatttta tataccattg
     3061 gaataattat ttctggccct tagtaatgac aaatacagat gcaataagaa ctttaccaat
     3121 aggagttgcg ctccttaaat cttcagaagg aattactgca tggaatgtaa taatggcagg
     3181 aaatataatt ctaattttac caataattct tgtttatata tttgcgaata aaaaagtaaa
     3241 agaagctttt atgtattcag gaataaaata aaaaattaaa gactaaattt ggaggatgaa
     3301 aatgaagctt aaaagaaaat tagcaacagg attagcaata acattattat taggttcttt
     3361 agttgggtgt tcaaattcta atgatgcaaa tgcatctaaa ggagaaaaga ctgatagcaa
     3421 tattacgaag atagatttct gggctccttt aggtggaact aatggagaaa ctgctcaaaa
     3481 aatggttgac caatttaata gtgaacataa ggatgtacaa gtaaatatgt taaaacagaa
     3541 agattactat gaaaatgcaa caaaactaca ggcagcctta acatctaaag accaaccaga
     3601 tgtaacatta ttagaaataa ctcaaacagg tacattcgca tcagcaggtg ctttagtaga
     3661 tatgagtaaa tattttgata aaacatatca agaaagattc ttttctggtc ttttaacaaa
     3721 ttcatattat gaggataagt ttgttggtat gccatttaat agaagtacac caatattata
     3781 cataaataaa gatatggcaa ctaaagcagg tcttgaccct tcaggtccaa aatcatggga
     3841 agagcttaag gattatgcta gtaaaatgac aaataaatca gaagatactt atggttttga
     3901 aacgccaata gatatttggt tctatgaagc tatggtaatg caaagtggag gagaaataac
     3961 taatggtaaa aaagtagcat ttaataatga ggcaggacaa gcaccagtta agttttggca
     4021 agatatgatg aaagctggaa taatgaaaat gcctcctgga gaagattata atgcctggga
     4081 cgtagcaaaa caagattttg taaatggtaa agtaggtatg atatttacat caacagcaga
     4141 tttagcagga cttatgcaac aaacagaagg taagtttgaa atatcaacag cattcttacc
     4201 aaaaaatcaa aaatacgcaa ctcctacagg tggagctaat ctagttatgc ttgaaggtgg
     4261 aacagataaa gaaaaagaag caagtgcaga atttatagaa tggatgacac aaacagataa
     4321 aatcgttcag tttagttcat caacaggata cttaccaaca actgaagatg caacaaactc
     4381 agaaaagtta caaacattat ataaagaaaa acctcaatat aaggttgcaa ctgaccaatt
     4441 acaatatgct gttgcacttc caatgttaaa tggatacaag gaagcaactg ataagctaat
     4501 ggatgagatt aaaaaggggc ttactgattt aaatgcaaat ccaaaagatg tagtaagcaa
     4561 aggaacttct gcaatgcaag cagtaataga taaaacaaat aaataattat atagacttgt
     4621 aagttgatta aaagtattca ataatatata aatgaagagg taatgtaaat gacaaaggtt
     4681 atagtattat cagtagattc tttatttgaa aaagacttgg aatttgtaaa aaatctacca
     4741 aactttaaat ctatactaga aaattgctcc atagtaaaaa atataaattg tgtttatccc
     4801 actctgacat atccgtgtca tactagtatg gttacaggag tatatcctaa aaaacatggt
     4861 atatatcata atgaaaaatt tgacccaaat aaagaaaata aagactggta ctggtactca
     4921 aaggacataa aagcaaaaac tattatagat gtagcaaaag aaaataatct aactacctct
     4981 agtgtattat ggcctgtaat gggagcaaat ccaaatatag attataatat tgcagaaata
     5041 tgggctccta gtagagaaga tgacccaaga gaaacttttg aacgaagttc cagtaaggtt
     5101 ataatggacg gtatttacaa tagacattgt cattatatcg attggaaatt agaacccaac
     5161 atggatttat ttggagtaaa ctgttcagta gatataataa aggaatataa accagattta
     5221 atgctaatac atttagctac attggaccat acacgacata attatggatt atttaatgac
     5281 gaagtagata aagcattaaa aatgaatgat aaatggctag gagatattat tcaggctaca
     5341 aaggatgcag gtacatacaa agatacaaat tttataatac ttggggacca tggtcatctt
     5401 agagttgata aagttataaa tccaaatgtc ttgcttaaat caaatggatt tataaaagtt
     5461 gaaaatggag aagttaagga ttttgatgcc tatgtaaatt cttctggaat atcagctcaa
     5521 ataatagtaa ataacttaga taggttaaca gaattaagag aactgcttga agaatttaaa
     5581 gaagaattat tcatagagaa tatttttacc aaagaggaag catctaacct tggtcttgaa
     5641 ggtgattttg aaatagtgat tgaaggtctt gaaggaattt catttggaaa tgatttcgaa
     5701 ggaagtatta taaaagattc agatataaaa gattacaagt ttgctgttgc gacacatggt
     5761 catttaccta ctaaaggtaa tagacctcca tttatagctt ttggaccaaa tattaaaggt
     5821 ggtgtggtta tagaagaagg agacctgaga gcacatgcag caactatatt aaaaatgttt
     5881 aatttaaaat tagatggagt tgaaaaagaa gcatttgatt ttattatgta agttgaataa
     5941 agtatttatt ttccatgaat tatagacttc tattgcaaag atagaagtct ttttttatgt
     6001 tttagattta agtaaaattt aaatgggtaa aatatttaaa ataacttaaa caattttata
     6061 aaggtatgat ataataatac atgtcaggaa ctaatgttcg aaaaaaggag tgatactatg
     6121 gattttaaaa taaaatcaga cttcaaacct acaggtgacc aaccagaagc tattaaatca
     6181 atagtggatt ctataaatag aaatgagaaa ttttcgacat tattaggtgt aacgggttca
     6241 ggaaaaacat tcacaatggc aaatatcatt caacaagtta agaaacctac cctaataatg
     6301 gctcataata aaactctagc agcacaactt tacagtgagt ttaaagaatt tttcccagac
     6361 aatgcagttg aatactttgt aagttactat gattattatc aaccagaagc atatgtagct
     6421 cacagtgaca cttatataga aaaagatgca agcataaatg atgaaataga taaacttcga
     6481 cattctgcta cagcatctat tttagagaga agagatacta taataatatc atctgtatct
     6541 tgtatatatg gacttggtga cccaaaagac tataaagaac ttatgttatc tataagacca
     6601 ggtatgcaaa gagacagaga tgatgtaata aaaaggttaa tagagataca atacgaaaga
     6661 aatgacataa actttacacg aggtaccttt agagttagag gagatatact agaaatattc
     6721 ccagctagta atgatgaaaa agcaataaga atagaatttt ttggtgatga ggtagataga
     6781 ataacagaaa ttgattatgt aacagggaag attgttggta ctagaaatca tgttgttata
     6841 ttcccagcat ctcactatgt aacaacgcca gaaagaattg aaaaggcaat tgtagaaata
     6901 gaaaacgagc ttcaagaaca aattaaattt ttcaaggaaa atgataggct attagaagca
     6961 caaagaattg aacaaagaac taagtatgat atagaaatgt taaaggaaat aggattttgt
     7021 caggggatag agaactattc tagacatatt acaggaaggt cagaaggaga aagaccatat
     7081 actttaatgg atttcttccc agatgattat ttgataatta tagatgaggc acatgtcact
     7141 ataccacaag taagaggtat gtatgcagga gacaggtcta gaaaaacatc acttatagag
     7201 aatggtttta gattaccttc tgcacttgac aatagaccat taaattttca agaatttgaa
     7261 ggaaatataa atcaaatgtt gtttgtaagt gctacaccag gaccttatga aattcaacac
     7321 tcagagacaa tagcagaaca gataataaga ccaactggcc ttttagaccc aattgtagaa
     7381 gtaagaccta taaataatca aatagatgat ttggtaggag aaataactaa aactatagaa
     7441 aaaaatgaaa gagtgcttat aaccacttta actaaaaaaa tgagtgagga cttgactaac
     7501 tatcttaaag aaatagggat aaaagttaaa tatttacact cagatatagt tacattagaa
     7561 agaacagaaa taataagaga tttaagatta gggaagtttg atgtccttgt aggaataaac
     7621 ttacttagag aaggattgga tataccagaa gtttcactta tagcaatttt agatgcagac
     7681 aaagaaggtt tcttacgttc cgaaacagct ttaatacaga ctattggacg tgcagctaga
     7741 aatgaaaatg gaagagtaat tatgtatgca gacaggataa ctgactcaat gcaaaatgct
     7801 attgatgaaa caaagagaag aagagatata caaaatttat ataatgaaga gcacaatatc
     7861 attcctaaaa ctatacagaa aaatataaga gatagtatag aagctactaa ggttgcagag
     7921 gaagaagttg tatatggaat atctgataca gatgataaag acgaaataag agctaatata
     7981 gataaattaa aatcagagat gatggaagct gctcaaaact tacaatttga aagagcagca
     8041 gaacttagag ataaagtgaa acaattagaa gaaaaattgg aaaaatagaa aggtaaaaac
     8101 aacattatgg aagataaaat tataataaga ggagcaaaag aacataattt aaaaaatata
     8161 gatttagaat taccaagaga taaatttatt gtttttacag gtttgtcagg gtctgggaag
     8221 tcttctttgg catttgatac aatatatgca gaaggacaga gaagatatgt agaaagtcta
     8281 tctgcttatg caagacaatt tttaggtcag atggaaaagc caaatgtaga atatatagag
     8341 ggtttatcac cagctatctc aattgaccag aagactacct caaaaaaccc acgttctacg
     8401 gttggaacag taacagaaat atatgattat ttgagactat tatttgcaag agtaggagat
     8461 gtgtattgcc ctacatgtgg tgaaaaaata agtcagatga ctattcaaga aatagttgat
     8521 aaaatgctag attttccaga tagaacgaaa ttacaaattc tttcaccaat tgttagagga
     8581 caaaaaggta cacataaaaa agtctttgat aatatcaaaa aggaaggatt tgtaagagtt
     8641 agagttaatg gtgaaaacta tgaagttagt gatgatataa tcttaaataa aaatcaaaaa
     8701 cataatatag aagttgtagt ggacagaata gtaattaaag atggaataga atcaagacta
     8761 gcagattcaa ttgaaactgc agttaagcta tctgatggtc ttgtaatagc ccaaataata
     8821 gatggagaag aaattctttt ttcaactaaa tttgcatgcc cagaacatgg aattggcata
     8881 gaagaattat ctccaagaat gttttcattt aatgctccct ttggtgcttg tgatgtttgt
     8941 aatggtcttg gggaaagtaa agaagttgac ccagaattag tagtaccaaa caaagattta
     9001 tcaataaaac aaggtgcagt ggcagcatgg ggttcaacag gtgttaatga tgacacttat
     9061 tacagcaaaa tgataaaaag tttagcagag cattttaatg tatctttgac aacaccgttt
     9121 aaagatttac cagatgattt tgtggaggaa ttactttatg gtaaagataa tataattgta
     9181 gagtttactt atgagtcaaa atttggagga actagaaatt ataaatcata ctttgaaggt
     9241 gtaatagtaa acttagagag aagatataga gaaactaatt cagattacat gagagataag
     9301 attgaagaat acatggcaga gagaccatgt ccaaagtgta aagggatgag acttaaaaag
     9361 gaagttttat ctgttttagt tgatggaaaa aatataatgg aagttacaaa cttatctgta
     9421 aatgaattga ttgactttat ggaaaatata aatcttacag aaaaacagag atttatagct
     9481 catgagatta taaaagagat aaaaggaaga gctatgttct taagagatgt aggtcttgat
     9541 tatttaaatt tatcaagaaa agctggaact ttatcaggag gagaagcgca acgtataaga
     9601 ttagctactc aaataggttc tgcattggtt ggagttttat atgtacttga tgagccaagt
     9661 atagggcttc accaaagaga taatgatagg cttataacta ctcttagaca tctaacagat
     9721 ttaggaaata ccttaatcgt tgtagagcat gatgaagaca caatgagaga agctgattat
     9781 gttatagaca ttggtccagg tgcaggtata catggtggtg aaatagttgc acagggtact
     9841 ttagatgaaa taattgaaaa tgagaactca ataacgggtc aatatttaag tggtaaaaaa
     9901 caaatattgc ttcctgagac tactagagaa ggaaatggaa gttttataga aatagttaaa
     9961 gcatcagaaa ataatctgaa aaatattgat gtaaagttcc cattaggtaa gtttacatgt
    10021 ataacaggtg tttcaggttc cggaaaaagt acattaataa acgatatact ttacaaaggt
    10081 gttgcaagta aagtgaatag acttaagcaa agagctggta agcataagga gataaaagga
    10141 attgaaagta tagataaagt tataaatata gaccaaagtc ctattggaag aacacctcgt
    10201 tcaaatcctg ctacttatac aggtgtattt gattttataa gagatatttt tgcatctact
    10261 aatgaagcta aagcaagagg ttataaaaaa ggtagattta gctttaatgt aaaaggtggt
    10321 agatgtgaag cttgtaaggg tgatggaata ataaaaatag aaatgcattt cttaccagat
    10381 gtatatgttc cttgtgaaat ttgtaaaggt gaaagataca atagagaaac attgcaagta
    10441 aaatacaaag ataaaactat atctgatata ttagatatga atgtggaaga agctgttgag
    10501 tttttcgaaa atataccaaa tataaaaaga aaattagaaa ctttaatgga tgtaggactt
    10561 tcatatatta aattaggaca gccttcaact caattatcag gaggagaagc acaacgtata
    10621 aaattagctg ctgaacttag taaaagacca acaggaaaga ctttgtatat attagatgaa
    10681 cctactactg gacttcatat ggcagatgta gacaagctaa tccaagtatt acaaagacta
    10741 gcagatacag gaaatacaat tatagtcata gaacataatt tagatgttat aaagacttgt
    10801 gattatgtaa tagacttagg accagaaggc ggagataagg gtggaactat agttgcaact
    10861 ggaacaccaa aagaagtatc taaagttgat ggttcttata caggtcaatt cttaaagaaa
    10921 tatttttctt aagttatata aattgtgaaa aattttagag cagatatacg ataaactgtt
    10981 ttgttataag taaattagtc aaatataagt tgtaaatagt aatacaaagt aaagtccata
    11041 ttactcttca tggttaaaat tgttttcaaa tcaattatag aagggaatat gggctttaac
    11101 ttgtttaaag cactggaaat tgtataactt tttaaaattc atattttagg gtataatttt
    11161 agtataaaat ttaatgtttt ggagggcgag atgtttgata tacaagagca tttaaaaaaa
    11221 ttaccttctg agcccggagt atatttaatg aaagacaaat acgaccatat aatatatgtg
    11281 ggaaaagcca tatctctaaa aaatagggta aggcagtatt ttcaatcttc aaaaaaccat
    11341 acatctaaag taaaatctat ggttaagaat atatataagt ttgagtatat tataacggat
    11401 tcagagttag aggcattaat acttgaatgt aatttaataa aaagatacag accaaagtat
    11461 aatgtagtgc ttagagatga taaaacttat ccatatataa aagtaactac gaatgaagat
    11521 tatccaagaa tcttaaaagt tagaagagtt cttaaagata aggcaaagta ttttgggccc
    11581 tataccaata taactgctgt gaatgatacc ttggagctta taagtagtac gtatccaata
    11641 agaagctgta aaatagatat agataaagca ataaagaata agacaagacc ttgtttaaat
    11701 ctacacataa ataaatgttt aggaccttgt acaggaaatg tttctaaaga agagtatgga
    11761 aaaatgatag aagagattat aatgtgttta tctggtaaag aagaaaaatt gatggaactt
    11821 ttaaaggaaa aaatgaatga gagttctatg aactttagat ttgaagaggc tgctgtatat
    11881 agggataaga taaagagcct tgaagagatg atacagaaac aaaaaataga tgctactgta
    11941 agtgacttaa accaagatgt ggttgcaatg gcaagagctc ataatgaagc ttgtgtccag
    12001 gtatttttta taagaaatgg taagattgta ggtagggaac attttatatt agaaggggta
    12061 atggatagtc caagagcttc tatattaagt tcatttgtaa aacaatttta taatgaacaa
    12121 gagtatatac ctaaagaatt aattatagaa gatgagatag aagatagttc tatattagaa
    12181 gaatggctgt catctaaaaa ggggcaaaaa gtaacaataa gagttcctca aaagggagag
    12241 aagaaaagct tagttgaaat ggttaggaaa aatgctgtag aatatcttga aaaattttct
    12301 gatatgaata aaagaaaata tgaaaaaagt attggagcat tggaagaatt aaagcaaata
    12361 ttaaatctag aaaagttgcc aataagaata gaggcttatg atatatcaaa tatacaaggt
    12421 gtagattcga ttggttctat ggtagtgtat acgaatgcta aaaaagataa gaaggaatat
    12481 aggagatata aaataaagac agttattgga cctaatgatt atgattctat ggctgaaatt
    12541 gtagatagaa gattaaaaca tggaaattta cctgacttga tattgttgga tggtggaaaa
    12601 ggacaagtta gtgcagtaaa aaaagtacta gaactcaatg atgtagatat acctctttgg
    12661 ggaatgtaca aagatgataa gcatagaact aaaggattga tatgcaaaga aaaagaaata
    12721 gaactagata aaacaactaa tttatataga tttattgcta gtatacaaga agaagttcat
    12781 aattatgcaa taacatatca tagaagtcta agaaataaag ctttgacaaa atctatctta
    12841 gatgatatac aaggaatagg tgaaaaaaga aagaaatcat tattaaatca ctttaaagat
    12901 gtagatgcta taaaaaaagc tactatggaa gaacttttag aagtagatgg aatgaataaa
    12961 agcattgcag ataatgttta taattttttt agaaaggaag aaaattaatg gaaactagca
    13021 ataaagtatc aataagagag ataataaaag agttgaagtt aaaagtagta tacatgcctg
    13081 atgatgtaga ttattatgtc tactctcaag atataaatag accaggatta cagtttgcag
    13141 gatattttga ttattttgca tatgaaagaa tacaaatagt tggaaagaca gaatataatt
    13201 tttttggatt aatggatagt catattcgag aagaagtact agatagattt ttctcatatg
    13261 aaataccagc gttaataatt tctagagact tggaaatcaa gccagatgta attgaaaagg
    13321 ctaaaaaata taaaagaata cttttgagca caaatagaaa taccactaga cttataaata
    13381 gactgtcaaa ctatttggat aataagttag caccacacac aacaattcac ggggtgctag
    13441 tagatattta tggtataggt gtactgataa aaggtgaaag tagtatagga aagtcggaaa
    13501 cagcattaga actgattcaa aggggtaata gattagttgc agatgatgct gtagaaataa
    13561 gaaaattaga tgaaagtatg cttgtgggtc agtcacctga actaataaga cattttcttg
    13621 agataagagg aataggaata atagatgtta gaagtttata tggagtaggg gcaatcaaaa
    13681 attctaaaat gatagattta gtagttcatt tagaagcttg ggatgaaact aagtattatg
    13741 atagactagg actggataaa gaatatgaag aaatacttgg tgttaatgta gagaaattag
    13801 ttgtacctgt taaacctgga agaaatactg ctatgatatt agaagtagca gcaatgaatt
    13861 ttagacaaag aggaatggga tacgatgcag ctcaagaatt tactaaaaaa ttatcaaaat
    13921 taatcgatac taagtagtac tgaataatag tgaactacac atcatttaat taaatattga
    13981 ttaggattat atcctacacc ttataaatta aaatcaagtt gtat
//
LOCUS       DAEOSP010000101        13956 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.101, whole genome shotgun sequence.
ACCESSION   DAEOSP010000101 DAEOSP010000000
VERSION     DAEOSP010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13956
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.101"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            95..325
                     /locus_tag="KRH54_002246"
     CDS             95..325
                     /locus_tag="KRH54_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579903.1"
                     /translation="MRLDMDSMLYVLIYMTLLVSIIGSIFYFCKDLLNHKIKYVLIRD
                     IFVNRITYSEERLPKQYIVFQKYDIWRYCSLF"
     gene            382..1032
                     /locus_tag="KRH54_002247"
     CDS             382..1032
                     /locus_tag="KRH54_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBG8579904.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEYIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            1235..1906
                     /locus_tag="KRH54_002248"
     CDS             1235..1906
                     /locus_tag="KRH54_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8579905.1"
                     /translation="MAKILVVEDEKRMQNIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            1903..3033
                     /locus_tag="KRH54_002249"
     CDS             1903..3033
                     /locus_tag="KRH54_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8579906.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLIDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            3464..4309
                     /locus_tag="KRH54_002250"
     CDS             3464..4309
                     /locus_tag="KRH54_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8579907.1"
                     /translation="MIKVRELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSIFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            4309..5337
                     /locus_tag="KRH54_002251"
     CDS             4309..5337
                     /locus_tag="KRH54_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8579908.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFFICDLCFTVLLVV
                     FNIGFLEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            5340..6353
                     /locus_tag="KRH54_002252"
     CDS             5340..6353
                     /locus_tag="KRH54_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8579909.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVF
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(6870..8120)
                     /gene="cme"
                     /locus_tag="KRH54_002253"
     CDS             complement(6870..8120)
                     /gene="cme"
                     /locus_tag="KRH54_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBG8579910.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(8206..8952)
                     /locus_tag="KRH54_002254"
     CDS             complement(8206..8952)
                     /locus_tag="KRH54_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8579911.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSSPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(9105..10127)
                     /locus_tag="KRH54_002255"
     CDS             complement(9105..10127)
                     /locus_tag="KRH54_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBG8579912.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKIVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(10127..12547)
                     /locus_tag="KRH54_002256"
     CDS             complement(10127..12547)
                     /locus_tag="KRH54_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG8579913.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSLKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLEQGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(12633..13553)
                     /locus_tag="KRH54_002257"
     CDS             complement(12633..13553)
                     /locus_tag="KRH54_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG8579914.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
BASE COUNT         4934 a         1810 c         2192 g         5020 t
ORIGIN      
        1 atactataat tatttaaaac taaatataaa tgttggaatt atttaggact tgaaattgtc
       61 taaaaataaa tacaagtgat gaagaggtga tgtgatgaga ttagatatgg atagtatgtt
      121 atatgtacta atttatatga cattattggt atctatcatt ggaagtatat tttatttttg
      181 taaagacctc ttaaatcata aaattaagta tgttttaata agagacatat ttgtaaatag
      241 aattacatat tctgaagaac gactgcctaa acagtatata gtttttcaga aatatgatat
      301 ttggaggtat tgtagtttgt tttaatttga aatgaatcta ttataaattc taaagtttaa
      361 aaataaaaag gagagaataa aatggatttc atttcaagta tattgcaagg attattaagt
      421 atattattta attttacagg tgattttgga atttctattg tacttataac tttaatagtt
      481 aaattagtac ttatgccact atcattaaaa caaagatttt ctgtaaaaaa acagcaagaa
      541 ttagctgaaa aaatggaata tataaaagaa aagtataaga atgatactaa agaattggaa
      601 aagcaaatac aagtaagttc tgtagaaagt atgaagagta tgctgggatg tcttatagtt
      661 ttggttcaaa tgccagtagt atatgcttta tatcatgtat gtttagatat gacaaaggaa
      721 tttacaacaa ttataattcc atggattgct aatttaggta catttgataa tttgtttata
      781 ttaccttgta tatatacatt aattatgtta gcttcaaatt tagtgaatta tataccatat
      841 ttaaaaataa attctcaagt aaagtttaac aaacaaatgg ctgtggtgac taccataaca
      901 agtcttatac taactgttag aactccagta gccataggtc tgtactttat aacaagtgca
      961 atttattcat taattgaaga tatttgcttt agaatatatt ttaatcgaaa aaatagaata
     1021 gtgacaaaat aatataatta ttattagaaa tattttagcc tgctaacata gaaaaagtta
     1081 gtaggctatt tttatctatg atatcaattc cattaagaat agtctaaaaa atactatcaa
     1141 aatgctataa aaaatactat aaaaaaatag ttgaaaaata aataaattaa tataaactaa
     1201 aataaagcct acaaaaaaga ggtgataaat agaaatggct aaaatactag ttgtagaaga
     1261 tgaaaaaaga atgcaaaata ttattgtaga atatatgcaa aagggaggat atacttgtat
     1321 aactgctgat gatggagtcg aggcacttac aatattaaag agcaacaata tagatttaat
     1381 gatattggat attatgatgc cgtatttaga tggattttca gtgtgtagag tttcacgtga
     1441 aatgactaat atacctatta ttatattgac agctaaaggt gaagaggagg ataaattaaa
     1501 gggttatgaa tatggcgcag atgattatat aacaaaacca tttagcccaa aagtactttt
     1561 agcaaaagtt aatgctttgt tacgtagata cactactgat attcccaaaa attcattgag
     1621 tttaggaaag atttttatta tggtagcatc aagacaagtg tatgtagaag ataaattgat
     1681 tgatttaaca tataaagaat ttgaattatt gcgtttattt atggaaaacc ctaatcaagt
     1741 tttttccaga gagaaacttt taaattgtat atggggatat gattttgaag gaaacacacg
     1801 aactgtagat acacatataa aaaccttacg caaaaagctt ggtagtgaag gacaccatat
     1861 agttacatta attcgttcag gatataagtt tgaggttaag gaatgaatag tttaagaaag
     1921 attatagaaa aactgaaaaa tgtagatatt tcaaattgga ttaaacaaaa ttttggacta
     1981 tattcagtta gaaaaaaagt gtttttactt tctaaattat caggagcgtt aattatatta
     2041 ttttatctgg ttgttgaaga attttcgata aactctaaat ttgaattttg gatttggttt
     2101 ataatactgg ttatatttat tatgagtatt gatttcttat tgggaaaatt tatttcagaa
     2161 ccaatcacat ctataaataa atctgcaaaa agtatgtccc aacttgattt ttctaatcca
     2221 tgtactgtaa atactaatga tgaatttgga gaactttcta ggagcctgaa tacaatgtct
     2281 acaaatctac aacaggcatt atcagaccta gaaagtgcaa atattcaact tgaaaaggat
     2341 gtaaataagg aaagaatgct attggaacaa agaaaggagt tggtagatac aatttcacat
     2401 gaaatgaaga ctccacttgg cattatacgt gcgtatacag aggggcttat agatgaagtt
     2461 gatgaagaaa aaaagaaaaa ttatatgaat gttataatag aagaaactga tagaatgaac
     2521 aatatgattg tatcattatt agacttatct gcattggaag caggagtttc taaacttaac
     2581 ccagagcgtt ttgattttat agagtttgtt gaaactgtgg caggtcgtct tttaattgat
     2641 ataccagatg ccaattttta ttttacatat gatttgccag agtatgaagt ttttgtagtg
     2701 gcagataaaa tgagaatgga acaagtagta gagaatctag ttattaatgc aaagaagcat
     2761 gttgtatata atggtaattt agatttatcg attacatgtg aaaatggctt gcttttattt
     2821 gaaatttata atgatggtag gtttatagag caagatgaga tttcaaagat ttggtctaag
     2881 ttttatcgta gtgttcaatc tcagcgtaca ggaggttcag gtctagggct tgcaattgtt
     2941 tcacaaatac ttacaatgca agggttagaa tatggagtga ataatcgtga tagaggagta
     3001 gagttttatt ttatgattcc aattgatgaa taatttagta ttaatttata aattataagt
     3061 atctgatgta aaatattttt atttgtatac gtaagtattt aaagagaata aaataaaaaa
     3121 acttaatgaa aaagcttaat gaaaaagtct aataaaaagc ttaatgaaaa gcttaataaa
     3181 aagtcttgtt ttcctgatga aaacaagatt ttttattaag ctttttatta tttcacttcg
     3241 atttcacaag agagacactc tgatttcacc aaatagaaat aaaataggtt aaataaatta
     3301 aaaaatagaa gcgtaattaa ataaggttac aagggggagt acttatgaaa tggtatacat
     3361 ggttgactat tatattgata atattgcttt ctattccgct taaaattaaa tttataaaat
     3421 ggtgggatag acgacaaaaa aatgataaag gaggttatga tagatgataa aagtaagaga
     3481 gttaaaattt tcttatggaa aagataaaca gatattgcat ggacttaatt ttgaagtaaa
     3541 agaaggagaa atctttggat tccttggacc taatggttca ggaaaatcca caacacagaa
     3601 aatcttaaat ggtgttttaa aaggttacaa tggtcacgta tccatttttg gtaaggaagt
     3661 taaagcatac acagaatctt tatatcaaaa gattggtgtt ttatttgaat ttccttattt
     3721 atataccaat ttaagtgcaa ttgataattt agaatatttt tcgtcttttt atcctaaaaa
     3781 tcaaaggaga gacatatatg aactcttaga tttgttagag tttaagaaag agtttataaa
     3841 taagcctgta tcatcctact caaaaggtat gaagcaacgt attagcatgg caagagcatt
     3901 ggtaagtaat ccaaagttgt tatttttaga tgagcctact agtggacttg acccatctgg
     3961 agctgtacta tttcgtaaga ttattgaaga agaacgaaaa aaaggaacaa ctatcttttt
     4021 gactactcat aatatgctag atgcagattt aatgtgtaac aatgtggctt ttattgcaga
     4081 tggaaagatt atggtaatag ataaacccaa aaatttaaaa atgaaaaata gcaataataa
     4141 agttgaagtg gaatttgtat ataatggaaa tcgagatatt gaaattgtag atatacaaga
     4201 gttagaatct ggaataactt ttaaatatga tgaaatctta agtgtacatt caaaagaacc
     4261 tacactagaa gaagttttta ttaaatacac aggaaggatg ttagtgtaat gtattctaaa
     4321 ttttttacct tagtaaaaaa agatttttgg atgatgattt atggtaaatt ttttgtgctg
     4381 acacttggct ctctcatact ttattcgtgt tatattaatt ttggttatgt aaagtttatg
     4441 gatgaagata gttatcacaa taatgtatat ctatataatc cagagaatat aagcatgagt
     4501 gcatctccat taattaatca agtttcttct ataggtgaat taaaagcaaa acttgatgaa
     4561 gatacaagtg cagtttgtat tgatatgagt tccagtaagc cgaaagtaat tttacatgaa
     4621 agtattaaag gagttgacaa ttatagagct gactatgcat tgtcattatt atcatcagaa
     4681 aaagaccata atagcaaaat atcagacaac aatgcaataa ttcaaattaa tgataaggat
     4741 acatctgaga taaaacaaag aaaagacatg acttgtgaga ttttattctt tgaaattgta
     4801 gctattggat ttttagggat tgcctcttta ttatttaaag aaaagcagat gggagtaatg
     4861 cgtattactg gtattttacc tgtaaaaaga agtcttttta tattatcaaa gatatttgct
     4921 ttttttatat gtgacctatg ttttacagtt cttttagtag tatttaatat tggattttta
     4981 gaaggaataa aaatacttcc acaagtatta gttcaagcta gtattttatc aattattatg
     5041 tctttgattg gaataggatg ctcaatgtta ttgagagatt ttaagcaatt ttctcttata
     5101 tatcttgtgg ttgctgtatt cataactaca cctatatttc tatctgcaaa tacatctttt
     5161 gaaatagatt ggattaaata tcatcccttc tatcatgttt atagtcagct taaaaatgcg
     5221 tttttcatga atttttcaaa agaattagtt tattttgtag tgtgttcttt gacaataata
     5281 gttctatttt tacttgtaaa atatattttt aataaagaat tggttaggga gggataatct
     5341 tgataggatt acgttaccag ttaaagagta taaaaaaaga taaaatgtgt ataatttcct
     5401 tctttttacc agttgttatg gcgattttaa taaattttat gggaacaatt gatatatctt
     5461 cattaggaga gatacgtttt ggtatagtga aggatacact tcctgtacaa acagagcagt
     5521 ggttgaagaa gtatggtatg gtaagtgtat atgacacaaa aatgcattta atacgtgaaa
     5581 ttaaggatat taaaacggac acaattggag ttgttagaga tggtaaagga ataaaaactg
     5641 tgttttatgg aaatgagatt aatgctataa aaaatgttgg aaagacattg ccagagatgt
     5701 ttagactaaa gggtgattta aaagatgttg aggttacaat attgcctaaa gatagtattt
     5761 taaaagagtt tcaaaatgtt tttattgcta tgattttaat tactgctatg tttatgggat
     5821 gcacctttaa tgcaatgaat attatatcgg aaaaagaaga tggaatatca tttataaatg
     5881 aagttttgcc acagagtaga agtcagtata ttatacaaaa agtatttttg ggtcttttat
     5941 gtgggtgttt atcttcaatg ataactgtat ttatatgttt ggatatttca atattagata
     6001 tgataatgat gttgttactc gttgtatttt cagcatttat ttcatcactt ataggtttat
     6061 ttattggaaa gttgtcagaa ggtcttatgg taggaattgt ttatattaaa atagtaatga
     6121 ttttatttat aggtactcct ttgttaatat atcttctagg ggttaatacc agtggtatag
     6181 ggaatttatg ctatataatg ccttcagttg ctacatttaa tggtattatg gagattatta
     6241 atggtaatgt cattattatc aaagatattg taattttggc tgtacattgt gtagtatggt
     6301 ttatgatata tattatttta gataaaaaaa gaaagttatc ttttagtaga taactcagta
     6361 taattataat tgggacaatt taataggtga tttaatatac tttgtatagt gtaatttatg
     6421 tagtgcaatt tgtatacttt aatttatgta gtgtaatagt tagttttatt ttataattat
     6481 tcaatataat ttagtaagaa gttcagtaca attagagcta cttagtggaa aacttaatag
     6541 gtacttaaac tatataaata ctttattttc ctccatttgt actataaatt taatttatga
     6601 tataaatgga ggtattatta tatgatttga taaatttcat cattataacc tacaagtaaa
     6661 ggtattatta tataaagtta ataaagatag tatcaaaata ttatttgcta taaagagagg
     6721 gactcaaaat aaagataaac ctatttttca atgttgaaat gataaaatat atttgatttc
     6781 atataaaaaa ttttcttcat aaattaaaaa gtccatttta agccccttca tttttaacca
     6841 gataaatcga gttaaactat ttaaatatat tactcggatt catttgactt agatattcct
     6901 tgcttgtaaa attgttttga ataggggata gccaaagcaa aaaataaaag tataatccct
     6961 gctatgatta ttaaattatt aatagaaatt acatctgcca ttggtccgaa aatcatcata
     7021 ccaagaggaa taaaaccagt atatataata ataggtaagc taagaactct acctagcatt
     7081 tgtggtgcta catgctcttg caacatagtc atagctgatg cttgaatgat tggaattgaa
     7141 aaattcgtaa aaaacataaa tataaaaaat tgccaagctt gtgttacaag acctagaata
     7201 attgaaaaaa tagcatagga taatattgct agaaaaaagg tcttatttct atttttaaaa
     7261 ccaccaaaaa ctcctataat caatccacct aaaaccatac ctaaaaatcc aatcatttca
     7321 ataatagaca ggtatatata acccttgcca aatgttcgtt caataagaag tcctataaga
     7381 aatcctgatg gaacacaaag aagcatgaat gctccataag taaaaagcac ttttctaatg
     7441 aacttatctg aaaaacagta ctttatccct gataccatat ctgcaaaaaa tgagactttt
     7501 tcagtttgtt ttggtatatc atgtttagga attttgatga atattaataa tacaatagca
     7561 ataattgcag taaatacatc aattaaaaga acttgattta ttttattacc aaatccaagt
     7621 attgcagcag ctacaatagg aactataaat tgtactatag actgtagact actatttata
     7681 ccattaaaac gcattaaatg ttcttctggt acaagttgag gaatcattgc gtttacagca
     7741 ggtgtttgta caccacttcc tagtgaacgt ataacagata ttactgatat ggctattaat
     7801 tcacttgttg tgccatttga tgacatcata aatagaatta gaactaatgt agatatagca
     7861 attgttgaat ctgatacaat tatgagtacc tttcgattgt agtagtctgc ccatacacca
     7921 gcaaaaaaag aaattatcat ttgaggtata aaggagcata cagttaaaac tgttacccat
     7981 acacctgatg atgtctgaag tgtgacatac catatcattg ccatttgtac aagtgatgag
     8041 ccaaataagg aaatatattg acttgctaaa aaaagaatga cttttttctt ccatgtagaa
     8101 ttagtatttt tgttttccat cctattcctc ctttgatttg tgtgttgaat ttttcttgct
     8161 cttaaggata acacacaaat caaagtctgg agttagagtg gagggctagg aaaattgttt
     8221 tttcataatt tgaagatgag atttcatggt ttttgcatta tgttcagctt ctttcaatga
     8281 agttatcagt ttgtcacaca ctgagataat ttcgtcatca tcttttggag tgtcaaggac
     8341 agttttaata ttagtattag gactagaaga taaagcattt aacattcgta aaatagctga
     8401 aagagaatag tttgcacaac gaagtgcgct tataatttta agtcgtttta tatcttcatc
     8461 agtatataca cgataaccat tttgttttct ttttactttt aacaaaccat tcatttccca
     8521 atttcttaaa gtatctattg taacgtttaa gtgttctgca gtttcttttc tagttaaaaa
     8581 gagtttattt gtttctggag tatttccatt taaaatctgt gcaacaatag taagtgcttc
     8641 ctcggcatta gatttttcta tttctaattg ttgcaagtat tgatttacaa gactaatagc
     8701 cttatcaaag tccctattcg ctgatgttct tacaatctca agggcatctt ttcttaatcc
     8761 cctttgtacc acttcaattt tcaaggctgt acgagcaaat ctaaattgtt ctatatgaaa
     8821 atcattaaag atacgataac cattggtatt acgcttaggt ttaggtatta agtttagttc
     8881 ttcatataaa cgtactgtat taggatgtac accaattaaa tctgctacct ctgatgtcgt
     8941 gtaagtgtcc attagtattc aactcctttt agatatatag tattagtcat aataacacca
     9001 aacaaaagtc tggtgttata atggagggtt tggctatcta agagtaatta gtttatgaaa
     9061 gagtaattag tttatgaaag agtaattagt ttatgaaaga gtaattaatc tattatagaa
     9121 gtaagtatat atttttcaat gaactcccat ctatcacgag gtttaaacac tattttagat
     9181 ggtatggcta ttactaagtc atattgtgga atacaacaaa tagcatttcc tccagttccc
     9241 attgctgaat accacaaaac accatctttt ttacatagcc accacagata accgtattca
     9301 ttactattca ttttaactga ttcttcaatc cacgatttag atataatacg tttattattc
     9361 caaataccat catttaaata cagaaaacca aatcgcaaca tatctctagc actcaatgta
     9421 agtccccagc cacctataga ataaccctgt ctatcttttg cccaaccctt aagatttttt
     9481 ccaaagaaat cttcaaaacc aaaatctttc atatcataat ctggtataac tttcatacca
     9541 ataggtttaa ataagtactc attagcaaat tcacgagcag attttccagt agtttttgtt
     9601 ataatagctg aaagtaggtg cgttccagct gaagaatatt tgaaagtacc aatttcgcct
     9661 ttttgaccaa gttgattcaa ggtatatttt atccaatctt tttgtttgca aagtctttct
     9721 aaagactcag tcatattttt aaaagggtat ggtgcagtca ttgtaagtag gtgacgtata
     9781 gttactgctt ttttacatat ttcagaagga ctacaagtat attcaggaaa aaaatcaaca
     9841 accttttggt caacatcttt tatatatcct ttatctattg caatacctat taaggctgat
     9901 ataatacttt ttgtaactga tgcaacatga aaagtatcat cagcattata tccattatag
     9961 tacttttcaa agacaatttt tccttttctt gcaataatta taccatttat attgctatat
    10021 tgtgagatta ttaaaggttc aagttgtgaa agcttttctg aactcatacc aacacttgaa
    10081 gggtctactt tttcaaaaat taagtctaag caattgtttt tattcattaa agatacctcc
    10141 ctgttataga ttttggattt tcttttagag tttttggaga accttctgca attatataac
    10201 ctcctttgtc accaccttct ggaccaagct caacaatcca gtcacaaaca tttaatacat
    10261 ctatatcatg ttcaacaaca attacagaat ttccattttc aaccaattca tctaataatt
    10321 gaatcaactt agaagtatca tatagactta gaccagtagt tggctcatcc aatacataaa
    10381 ggatattacc atttcgtttt ttaccaattt cttttgcaag tttgattctt tgggattcac
    10441 caccacttaa agtcggtgta ggttggccta attttatata ccccatacct atttgttcta
    10501 atacatttaa gtgtgaaaca atatttggaa tatctttgaa gaaatgtaca gattcagaaa
    10561 cactcatatt taaaatatca tgtatatttt tatttttata tttaatagat aaagattcat
    10621 cattaaatct ttttccctga cattcactgc atatatggtc tatacttaga ttatttccca
    10681 accagacctt ttcatatcca ctaccactac atttaggaca agcccctttg gagttaaatg
    10741 agaaatgacc agctgtaaat ccttgttcta acgattctgg ctgacctgca aatagtgtac
    10801 gtatcttatc ccatatacca atataagaag caggagttga gttcatattt ttgcctatag
    10861 gagattgaga tatctctgaa tatccagaaa tatgtttagt accaatcagt ctatctgcaa
    10921 tagtttcaac aactgcataa tcgtcttcct cacctatttc actctctgaa gaatttattt
    10981 gattattgat agtatggtta ttaaattggc ttctaagaag aggaagtagg gtgtctgata
    11041 taagtgaact cttgccacta cctgattttc cagatattcc aaccatagca cccaaaggta
    11101 atgatacagt tacattttta agattatttg tatttgcatt ttgaatagtt atacaaggta
    11161 ttttatcagt atgtgtaatg tttttattgc ttgttctatt aggcattgga tattttccag
    11221 atagatactg tccagtaagt gatttgtcgg attgaagtag accttctaaa tctccttgat
    11281 atactaattg accaccttca acaccagctt ttggaccaat atcaataata tgttctgcca
    11341 tttcaattgt gtttttatca tgctcaacta ctatgaccgt attgcctaaa tctttaagtt
    11401 tctttagtga ttttaagatg tctatttttt ctgattcatg caatccagct gttggttcat
    11461 caagcacata aattaatgag tccatctctg aatcgagatg tgaatttaaa aatagtcttt
    11521 gcatttcccc accactcaaa gttgacattt ctcggtataa ggatagatga cctaatcgtg
    11581 attctattaa atgttgagtt ttggataaaa tatcatttac aagatttgta ccaaatgtgt
    11641 ttaaattacc ttgatttatt aagagtttaa ggaaatcatc aatttctaaa atcgtcattt
    11701 ttccaagttc tccaatatgt tttccgttta aaagtactct acgtgcttct tcaccaattt
    11761 taaaaccatt gcaatctgaa caggcagtca tagtataaac tccatttaaa tcttcgcccc
    11821 ttttgtaacg attgtgaaat acctttgata aagaggaact ttttttacca ttttcataat
    11881 gaccatagag tacttcattt cttatttctt caggtagttg taagaattgt atgtctaaat
    11941 aatcattgaa ttttttagaa aatactctca aatatccagg tgtaatcttt gctctatcta
    12001 agacttgttt tagggtatca tgcttatcaa ggactaattt ttcaatatta atctcaaaat
    12061 atgcaccatg tccagagcac ttcatacaca ttccatctga agagttgtat gagaaataac
    12121 ttggtcttaa tcgttcttcc tcatatccac aattattgca aattagatta tcgcttacag
    12181 gagtatcaca catagtacac attatttgac cttccactga atacaatagt gtaagcatgt
    12241 tgagaatatt ggttcttgtg cctactgttg aacgaggatt gctctggcga ataatatttt
    12301 gttgaacagc tatagtggga gctaaacctt gaatattatc aaacttatct tcactatcaa
    12361 tacctgcaag tatacctaaa gattgcagat attgtttacg cccttcctca aatacaatgt
    12421 caaacataag gctagactta cctgagccac tgacacctgt tgcaacaaca agcttatctt
    12481 ttggaataga aacatctata tttttaagat tgtgtatctt tgctccatta atttttattt
    12541 ctttcattgg ttcacctcat tttatcaatt ttatcaaacc gtaaaatagg gatatattaa
    12601 tccttaatac aagaatagat taggtgaggt tattaaaaag gttctatagg taaatatata
    12661 tcgaccaagt gtctattttg agggtgattg tgtgggttgt taagatatac ttcaaaaggg
    12721 aaggaggcac gtagcttata attaccattt gatagccatt cattatacat aaaatcccac
    12781 gcatcactat attcagattt tgaaatatca aaatgtccca caagatattt tccagaaggt
    12841 attatcatat tgccaagatt gtcattctct ttgacaacaa tgttatctgg aatggtcatg
    12901 caaatactag tccttaattg atgctcctgt gtaaattgtg gattatcatg ataaatggct
    12961 aatatttgtg tgttttcaag tccaagtaga ttctgctcac ttgcatatgt gaataaccgt
    13021 tctaaaagtt ttgggaaagt actggttaga ttaccatatg aaccagtata tctagtgtaa
    13081 atagtattca tattatcaag ttctagaatt tcaatttcac ctttaacctc tctagtattc
    13141 ttacatttag gttttgatat gcttctattg tactgagaaa ttttatatgg ttctttgcaa
    13201 ttcttgctat aattgtttct atattttata gggcttattt tatagtaatt tttaaatgct
    13261 ctcgagaaaa ctgctgaatc agtaaagccg aagtagtgtg caatatcagt aacagtcatg
    13321 tcagttctat gaataagaaa agcagttgca ttttctagtt ttagcctatt tacatattgt
    13381 gccaaagttt ctttttctat tgctttgaag attctatgaa aatgaaattt tgaaaaacct
    13441 gctatattag aaagttcatc aagagaaaga ggttcatgta aatgttcttc tatatagtct
    13501 tgcactttgc aaatacgatt tagatattct tttttacttt tagcatttgt catatatagc
    13561 cctccttatt gtgtaaaatg tgtatcaatt aatttatagc aatatagtaa taaaatttta
    13621 tgtttttgta atttatgata aataagatag cgtttaattt tttataattg ataaaagata
    13681 taagatgcct tatatttgtc tttgcatata agtatagtct tcgtaataat tagtatacca
    13741 tagtgattta tttttaattg tgtatttata taaatacaca acttatgata tggacgaatt
    13801 attatgcaaa taagctgtaa ttaaataaat tgcaatataa accagttgaa acataagtta
    13861 gggaaatatt atcaaagtta ttacttagca taagagtata gatggaataa aataagatta
    13921 aaaaattaca tactaaattt aataaaattt ttatat
//
LOCUS       DAEOSP010000102        13760 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.102, whole genome shotgun sequence.
ACCESSION   DAEOSP010000102 DAEOSP010000000
VERSION     DAEOSP010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.102"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(138..2108)
                     /gene="ftsH"
                     /locus_tag="KRH54_002258"
     CDS             complement(138..2108)
                     /gene="ftsH"
                     /locus_tag="KRH54_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG8579915.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2142..3530)
                     /gene="tilS"
                     /locus_tag="KRH54_002259"
     CDS             complement(2142..3530)
                     /gene="tilS"
                     /locus_tag="KRH54_002259"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBG8579916.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(3713..4141)
                     /locus_tag="KRH54_002260"
     CDS             complement(3713..4141)
                     /locus_tag="KRH54_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBG8579917.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(4158..4964)
                     /locus_tag="KRH54_002261"
     CDS             complement(4158..4964)
                     /locus_tag="KRH54_002261"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBG8579918.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(5166..5651)
                     /locus_tag="KRH54_002262"
     CDS             complement(5166..5651)
                     /locus_tag="KRH54_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBG8579919.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(5702..6361)
                     /gene="spoIIR"
                     /locus_tag="KRH54_002263"
     CDS             complement(5702..6361)
                     /gene="spoIIR"
                     /locus_tag="KRH54_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBG8579920.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(6506..7186)
                     /locus_tag="KRH54_002264"
     CDS             complement(6506..7186)
                     /locus_tag="KRH54_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8579921.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(7170..8060)
                     /locus_tag="KRH54_002265"
     CDS             complement(7170..8060)
                     /locus_tag="KRH54_002265"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBG8579922.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTKIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(8178..9728)
                     /locus_tag="KRH54_002266"
     CDS             complement(8178..9728)
                     /locus_tag="KRH54_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBG8579923.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(9822..10088)
                     /locus_tag="KRH54_002267"
     CDS             complement(9822..10088)
                     /locus_tag="KRH54_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBG8579924.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(10236..11096)
                     /gene="yabG"
                     /locus_tag="KRH54_002268"
     CDS             complement(10236..11096)
                     /gene="yabG"
                     /locus_tag="KRH54_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBG8579925.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(11180..12118)
                     /locus_tag="KRH54_002269"
     CDS             complement(11180..12118)
                     /locus_tag="KRH54_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBG8579926.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(12228..12389)
                     /locus_tag="KRH54_002270"
     CDS             complement(12228..12389)
                     /locus_tag="KRH54_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579927.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(12399..12896)
                     /locus_tag="KRH54_002271"
     CDS             complement(12399..12896)
                     /locus_tag="KRH54_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG8579928.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(13011..13721)
                     /locus_tag="KRH54_002272"
     CDS             complement(13011..13721)
                     /locus_tag="KRH54_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBG8579929.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
BASE COUNT         4110 a         2605 c         1318 g         5727 t
ORIGIN      
        1 aaataataaa aaaactgaaa gtatcaatta aattaataca tgcaaatata atttgcttat
       61 taatttaata ttatactttc agttttagta tttaatccac atcagataat aatcttaaaa
      121 tgaccaaggt cttaaaacta tttatcttta tcagatttat cttctaaatt tttatttata
      181 tcaataacat tgtttctttc ttcttcatct ttttctaatt ttatagttaa ttgttcttca
      241 acttctttag aagaattttc ctctgtaact gcattttcaa tttcattcag aggtaactct
      301 ttattaaatg cttttacaaa ttgttctgca tctaaagttt catatataag taaagcttgt
      361 gctacatact ctaatctatc catattttct tgtaatatac ttcttgtctt atcatatgca
      421 ccatcaacta tacgctttgt ttcttggtct atctcaaaag caacttcttc agaataattt
      481 ctcttacttg aaaaactatt acctaggaat acttcatcat cactgtcaaa tgacattgga
      541 ccaagtttag aactcattcc atactttgtt accatacttc ttgcagtagc tgtaactctt
      601 tctaagtcat ttgaagcacc tgtagataca tccttaagta ctagctcttc tgctactcta
      661 ccaccaagta aaactacaat attttctttc atttcatttt ttgtagcata gaacttatct
      721 tcaacaggaa gttgcattgt aaaccctcca gctcttcctc ttggaactat tgtaacttga
      781 tgaacaggac ttacttcttc aagtacatgg gcacaaactg catgccctcc ttcatggtat
      841 gctgtaagtc ttctttcttt ctcacttatt actttcgatt ttttagccac acctgctata
      901 acctttgtta tagcttcttc tatagtttcc atcttaattt tcttttctct ttttctagct
      961 gttaatattg cagcctcatt cattaagttc tctatatcag caggagtaaa tcctggtgtt
     1021 cttctagcca agacatccat ttttacatca tcacttaatg gtttatttct tgagtgaact
     1081 ttaaatatag cctctctacc ttttacatct ggtgtaccaa caacaacttg tctgtcaaat
     1141 cttcctggtc taagtaaagc tgggtcaagt atatctggtc tatttgtagc tgccatgatt
     1201 attatacctt gatttacccc aaatccatcc atttcaacta gaagttgatt aagagtttgc
     1261 tctctttcat catgacctcc accaagacct gcacctcttt ttctaccaac tgcatcaatt
     1321 tcatctatga atatgatagc tggagcactt ttcttagcct gttcaaataa atctcttact
     1381 cttgaagcac caaccccaac aaacatctca acgaaatcag aaccacttat actgaaaaag
     1441 ggaactcctg cttctcctgc aactgctctt gatagatatg ttttaccagt tccaggaggt
     1501 cctaccataa gcattccttt tggtattctt gcacccaatt caatatattt cttaggattc
     1561 tttaagaaat ctactacctc ctgcaaatct tctttttcct catctagtcc agctacatct
     1621 ttaaatgtaa ctctagtttt ttcatcatct ttatgaacct tagcttttga tttgccaaaa
     1681 ctcataacct ttccgcctcc accttgagac tgattcataa acacgaacca aataatcacc
     1741 atgaagaata ttaacagtaa tgtaggcaac atttcgacaa accaaggtgt agatggctta
     1801 gcttcaccac caaaagttag tttcccagcc ttagcttggt ctaacacctc atctgcaagt
     1861 ttatttccca tcacttctgt tggaacatat gacttaaact ttgtatttgt atcttttata
     1921 gtaccttcta cagaagtttg atttacaaag tataatcttg atatattctc atctgttaat
     1981 tctctatata cttcagagaa ttttaaatct tttatttttt ctgtaggctt acctgagaat
     2041 tgaactatac ctactataat tataaaaaca agtagataaa aacctgcccc ttttagcaac
     2101 ttattcaata tagaagccct cctttcatac attgctttgc cctataattt gttaaaatta
     2161 atttttaata cttcttttgt gttttcatca attttataat tttcactgat tttataatct
     2221 ccaacacata ttattccttc actatctgtg attataggaa ttttacatct ttcttctctt
     2281 gggattttta aatctatgaa taaatctttc acttttttac ttcccatagc aagctttatc
     2341 ttatcacctt gccttctgct tcttattact ataccccctt ttaccttatt aaaatcaaac
     2401 cctttagatg aattgtctaa tttcatactc ttatacctat ctatgctcat tacttgggtc
     2461 tcaataattt gttttaattc ttttatttta ataaacccat tactaggtac attgtaataa
     2521 aattcaattt cctcattaac aatttcctca ttcgttaata ttatactgtc tttttttcta
     2581 taaacaaata tatttcttgg aagagttaac attttattta cttttgaatt atcttctaaa
     2641 gacattatat cttctatatg tctttggtca acaaaattag tatctcctag tatatgtttt
     2701 atggaatttc taagaactct aactttgata gcatcatgta agcaaacaaa atcgtctaaa
     2761 tttatctcta caaagccttt ttcttttata tttgaaactt ctctaaattt agcttctgcc
     2821 tctttctcaa tataatcgtt atcacttttt aaactattgc tcatcctcac tatagattct
     2881 attacatttg aattaaaatt atccttcata tatggtaaaa ggtctaacct tatcttattt
     2941 ctagtgtata tattttctaa atttgtttta tctatcctcg gatttaaatt ataagcttca
     3001 caatactcct ctatctcatt tctttccacg tctaatatgg gtctaattat gcaattatct
     3061 ctaatataat caattccctt taaacctttt aaacctgttc ctctcatgat acgcatcata
     3121 accgtttcgg cttggtcatt gagattatga cctattgcta ttttattggc tttaatctta
     3181 tccttaattt cataaaacat ttcatatctt agcttccttg ctccttcttc taatgataat
     3241 ccctcatttt cacaatattt tggtacatta atagacttta caaagaatat aattcccata
     3301 tcctcacaaa gtttagatac atataatgca tctttttgag cttctatccc tcttatttga
     3361 tggtttaaat gtgctgcata tatttctata ttaaaatctt tttttaatct atttagtaca
     3421 tgcaataaac aaactgaatc aggtcctcca gaaagaccta aaactatttt atcgcctttt
     3481 tgtattaaat tatgtttatt tatagtactt agtaccttat caaatatcat atttatccct
     3541 cttacactta gttttattca cttttaagta tactttctat aaaaaaacgc taaacccttg
     3601 tatcaatttt aagaaattaa agtttaatat aaatataata ataccaaata aattaaataa
     3661 aaggtagttt tttcactacc tttataaaat ttctggaaaa tagccatagt tattatttag
     3721 tacctatgta tatatctatt ttattcctac ctaaaaataa atcttgtacc tttatattat
     3781 agtctatttt caattttatg tgttcccctt cttgtatatt tatatctaat tctcttgtat
     3841 caatatcaat taataaagta ccttgtgggg tcacatactt tgtagtacta ctttctccac
     3901 atttaaacag cattgttgag ttaatagcac caaacttttt gatactaact tgatttttga
     3961 gtatctttat agtattagtt atttctatat cttcttcttt ttccttgtaa ataacatata
     4021 ttccatcatt tttataaaaa attttaccaa aggcagtcat ctctatggtg tccatatttc
     4081 ctttttcatc atattgtcta gtatttatac ttatttttac actcaacgat ttttcctcca
     4141 tagattcttc atataaacta gtatttttgt atttctactt tttgaatctt atcaattttt
     4201 ttctttaaga actctcttgc tatatctgaa aatttttcag gtatatctga tacataatat
     4261 tgataagttc catgaatttc tgtattgttt ataatatttt gcacctctaa aatctttttt
     4321 aaatcctttg cagtctcttt agctggatta acaagtttta tatgctctcc aacttcttca
     4381 cctattgttc tctttaaaat aggataatga gtacatccta acactaaaga atctatattt
     4441 ttttctttca attcttgtaa atatatcttt gctgtcaatt tagccacttc tgtatttgcc
     4501 catccttctt caacaatagg cacaaataaa ggacatgctt tattaacaat ttctatactc
     4561 tcatctattt tagatatttc caagttgtat gcttttgaac ttatagttcc ttcagttcct
     4621 attattccta caattttgtt ttttgtagaa cttacagctg ttctagctcc tgcttctata
     4681 actccaatta tcggtatatc atatttttct tgagcttctt ttaagcttct tgcagtagca
     4741 gtgttacatg ctataactat agcttttaca ccttttgttt ttaaaaagtt tatagcttga
     4801 aaagtatatt taattatagt ttctttggat cttgaaccat atggtattct ggcagtatct
     4861 ccaaagtata ttatatcctc atttggtaat atcttcatta tctcttttag aactgttaat
     4921 ccacctaatc ccgaatcaaa aactcctatt ggtttattgt tcatctttat acaacaccta
     4981 ctttacattt ttgtgtttaa caacaactta tttatcgcta aaatttatat ttatattcat
     5041 aattaatata ttattaccta aatgattatt atagtttatt ttacacaaaa aataaaggag
     5101 ctatcttaaa ttagaaaata atttctacaa aaagacaact cctttcacta cttaaaaatt
     5161 taaatttatt ttgcaaacac atgatttcct attctcatat aaggattcaa tctatttatc
     5221 catttgcaag tggctatttc tggattccaa aaatatactg ctttattagt tgggtcagtt
     5281 ccataaagag cttcctgagc tgctttacgt gctgagtctg ttggtgccat ctttattgaa
     5341 ccatcaagca caactgagaa agcatttttt tgatatatga cttcttctat tgtgtctgga
     5401 aatctagggt ccaatactct atttattact acagctgcta cagcaacctg tccctcatag
     5461 ctttctcctc tagcttctcc tgcaaccaac ttagataata acatcaagtc attatcagat
     5521 aaatttatta cttctttctt tgctatattt ttttcttgtt tgtttacttt cttaatattt
     5581 tctgttggtt catatgcaaa tacctcttta ataggcataa atataaaagt caaagctaat
     5641 agtgtagcca tcagtttact ccacctgtcc atgattaaaa ccactcctta cattcttatt
     5701 tttaaaattt tttctctaaa tccttgacta cttcaagtac tttaaactta atttttactc
     5761 tacttgtttt ttttggtgta tcttgtttta ttaacttata ttctttatca gttaaaactt
     5821 ccttcaactt atcatctgta gatttatcta tcactccatt agattcatca acaaaacata
     5881 gtggagggaa cataacacac caccagtttt ttccttcacc tttcccaatg ataatcttta
     5941 atgctttata ttcaccagct ggaagaacta tattagaata ttgtttagta gggaaattgc
     6001 tatattgtat acctaccttt acactgtaat tatatccatt tttaagaata atatttttac
     6061 ttatttcttc cagactacta tattcctttt ttataatagc ttcactttct tttatacttt
     6121 ttgaattttg aagctttggc tgtaaatatt ttattacttc atctctaact tttagtttaa
     6181 gttcttggtc ttcatctgta ttgctgtttg ctataacatg aaatcttatt aatttatctt
     6241 tataatcttt tgatatattg tctacttttt ttacctcacc atttattact attgtcatta
     6301 ttgatatgac tgatataaga cttaatatta atagtcccaa tctaatctta atttttctca
     6361 ttgaaaattc cccctcaaga taaatttata tcatcaatta tgtccaatta aatttaattt
     6421 attcataata ttttttaata ctaataaaaa aagctatctt aaaatgatta caatcatttt
     6481 aaaatagctt tttaaacctt atattttata agtctttact aatcatagcc ttatctatca
     6541 taaactgctc tgctttttct atatgctctg tagcatactt cttggatagc tcactatcac
     6601 ctttaattat agcatcaagt aagtatttat gctcttcaac aatattatgt gatgtatcat
     6661 aagcagacat ataggtaact ctaaatctat atacttgttc ccacaatgag tttattaatt
     6721 ggattaattt tttattatta gttgctttaa atatacattc atgaaattct acatcaagtt
     6781 ttaataacgc atctacatcg gattccaaag tgcttttttt aaattcttct gatatatctt
     6841 taagcttttt aatatcttca tcactaattc tttcagcggc aagatatgct gctaagcctt
     6901 ctaggcttgc tcttacttct aatacatcca taatatcctt taatgacata tttgccacat
     6961 acgcaccttt tctaggcaac ataacgacta aaccttctaa ttcaagcttt cttattgcct
     7021 ctctcactgg agtcctactt actcctaatt gttctgctag ttgcacttcc attagccttt
     7081 gaccaggctt taattttcct tctaagattg cttctcttaa attctcaaag actacatctc
     7141 ttaatggctt gtaattatcc aagttcagtt tagttaaatt ctccacaaat ttcaacccct
     7201 ttttgactgc tattaacaac atacacctgt ttatattttt ttaataattc tttttttcca
     7261 attagagcat cttctttatt tttaaatagt ccaaaaactg taggaccact tccactcatc
     7321 atagaaccta aagcattatt tttcatcatt acttgtttta tatcacttat ttctttgtgt
     7381 ttacctatag taacattttc aagaatattt accatgcttt ctgatacagc ttttatatct
     7441 tcagatttta aacactctat caaatattta ttatttggtc tttttttaat attattcaaa
     7501 tctaatcctt gataaacttc tttagtagaa acgaataagt ctggtttgca tatcaatata
     7561 tttgtatcac atggtaaacc tttgattttt gttagctttt ctccaatacc ttgagctaag
     7621 gcaggtcttc ctgatataca aaaaggaaca tctgcgccca aattaagtcc tatttctttt
     7681 aattcatctt cagataatct aagttcccat aggtgattta aacctactaa tactgcggct
     7741 gcattagaac ttcctccagc catccctgct gctacaggaa tatttttttc tatgaatatt
     7801 tctacacctt tttttatata aaatttattt ttaagtattt tggcagcttt ataaacaata
     7861 ttatcttcat ctaaaggaat atctaaacta gtgcttttta cctttatttc atcttcgtct
     7921 aattctttta tctttactat atcgtataaa tctatcgttt gcattatcat ttcaacaaaa
     7981 tgataaccgt cttgcctttt tcccaagaca tcaatggata aatttatttt tgctctactc
     8041 tttagtctta taaaatccat tctatcctcc ttgtttaaag aataatgcat cactaacaat
     8101 aatattatac tacaaaaaat aaaataatag ggtattataa taccctatta tacttaatta
     8161 ctttaaattt tttaatatta atctactaat acgacttttt tttctaaaat aagacactta
     8221 ccaggcttaa ttggttcatc aagttttata tcattaagtt ctgcaatttc attttctgtt
     8281 gtattgtatt tttttgctat attccagaaa gtatctcctt ctttgcatat atatacgatt
     8341 atgctaggag ctttagataa atcatataca ccttggtctt ctcccttgat aataaaattt
     8401 tctgctttct tatctaatgc ttcagtaaat ctcttaattt tgataatcaa gtctatctgg
     8461 tccctattca aatcaacttc gactttatca atacatgctg tattaaacac agatgctgta
     8521 tcagttagat tatccatttc tatatcatgt tcaaatggaa tttcctcact aattttataa
     8581 accaatttta aaccttcaac tgggacaaat aaaatttcaa cttttatgat tccttgtata
     8641 atgcttttat ttccttcaat ataactattt tctatagaca tggttggaca aacgcttaca
     8701 atatctttta tttgtatatc atcattatca tttcttatgc cttctctaac cataaatgtt
     8761 tcttcactac gtcttaaagt cttatttaat tgaataggtt tgtggtcaaa ttttattatc
     8821 ttttgaggcg aatatgcatc ttgtaatact tctcttgtaa cttcatctgt aacttttacc
     8881 ttacaacaag ccatacaatc tatttctaaa agacctgtgt tgctttcact attttgcttg
     8941 aaaatatgat taaaatctga catagacaac aatacttctt cagtcatacc atcactaact
     9001 ccagggactt ctacaaattg agtaaactca atacctactc tatccaactc tacaagttca
     9061 ccctcatatg tacaagctaa cggatttatt tctaatactc caccaataat aactttattg
     9121 tcagtcactc tactttcttt tactttaaca catggattta aacttattat agattgaatt
     9181 tcctctgtgt ttattgttat agtatctctt atagaacttt ctgctttttc tattccaact
     9241 atatcttgaa aacaaatttc ctttctgtgc ttttgtatgc cttccacttg cgccacatct
     9301 tttacaatgt ctaacctttg cttttcaaac aaacttcccc ttatattcat aagagctcct
     9361 actttaatct ttctctcatt cattatagta caatccatat gctctacttc tgaaaataac
     9421 atgtattcca tatcttgaac aacattgtct ttttctatta cttcgtttat atctactttt
     9481 ccgtctacat tagaaatagt atttttatca tctgcaatat agattacatt ataattaaaa
     9541 ctacctctgc aaagtatctt cccatctgcc atttcaattt ttttaagtga tatgtatcct
     9601 tctgttttta caatttcata tacatctaat tttttgtctg gtacaactgc ctccgcttca
     9661 atgaatgttt gaaattttcc aaagtctatt ctattgtcaa ctttaattac atctttaatt
     9721 aattccatag tctacctcct taagtattat aaaaatatca cactatattt atatttaagg
     9781 agcaaaaaaa aaattacacc tatttttaaa taggtataat tttaacttat ttgtaattta
     9841 tcttgatctc taaaaacttg caactttaca ttagatgtta ataaatctga gtaactataa
     9901 gatactctag gatatccatt actttcatca tctaacttta taacaaacac gcttggataa
     9961 actttttcta gtataccctc ttttgtaata atctgctttc ttcctttatt agctttcaac
    10021 aatattttct ttcctatatg tctctctaag cttactctta tcttatctag agtttgaaca
    10081 gtagccacaa tatcaccttc tttaacgttt atatgtttac ataatatcac atataaacat
    10141 ttttgtcaaa tgtttaaact aattatttta tatatttttt gcaaaaatgt caatactatt
    10201 tttgaagata tacagtataa ttatgttaat ttttgctaat gtaatattgt ttttggcatt
    10261 gcccatctaa attttcctct ggtttcagaa ccaccaattc ccttaacacc tgtaatagtc
    10321 tgctctataa cctcatcaat aggaactact acatcaattc ttgaacaagc aattttctcc
    10381 actataaaaa cagggtctaa tgcatgtatc attatccttc caggcgaact tgcatagttt
    10441 gctcctgcac ttattatttt ttcataattt gattggcatg ctcctgcgaa tatgacaaga
    10501 ttatccaaat ttggttgcca tttacgtgct tcttttactg ttttaacaaa atttgaagag
    10561 tttctatagt tactcatatc ttctatatct cctcttttta ttgtcattgc atcatgacct
    10621 gttattacta gtatatccgg attatatttt tctagtagtg ctcttacttc tttatactga
    10681 ttttgctctg aaacagctac acctactgca ggtattccaa gttgagcata tacatccaag
    10741 caaattttta aatattcttt atctccatct atctgtagta cttttccagg cattccatat
    10801 gtgttaggat ttaattgaag ttttggaact gctctagtca ttcttttttg tctttcttta
    10861 gcttttctta ctgacttata taataaattt tctacatttt tatctattaa tatatctttt
    10921 atatcaggtg cttttactaa ttctaggtca tcaatataag catctgctat tactctaaat
    10981 gcaattccct ttagtatagc tattttctca ttattttcat ctacaccaaa agagactatt
    11041 ttaaatacta tatctttgtt atgtgacttt ctagctacaa tgtctcctac cttcatagtt
    11101 aaacctccaa taaattttta tcttagttta tactatgttc gatataaaac tttgtttcct
    11161 aatgtcaaaa aaacatttct tataatactt cttttatcac tcttaaagca tttttatagt
    11221 atatcttttc tatatcatct tcactaaacc cttctttttt tagaggttca tacagtttac
    11281 ccatctgaga tatatcttca atctcaactt ttgaatctat tccatcaaaa tctgacccta
    11341 gagatacaac atctattcct ccaacattta caatatgttt tatgtgtcta accatatctt
    11401 caagtttact ttcactttta tcacttaaaa ataagtggaa gaagtttata cctgtaactc
    11461 cacctttatt tgctaatact ttaatcatat catcagttaa atttctagaa tgattcatca
    11521 ttgctcttga attagaatgt gtagcaatta ttggtttaga agaaatcttt gcaatttcat
    11581 aaaatccacc atctgaaata tgagaaacat caaccaacat tcccaattca ttcatcttat
    11641 gtaccacttc tcttccaaaa tcagtaagcc ctttttctct atattctact ttattatgag
    11701 gaaagctcaa ttcattcaca tgattccaac ttattgtcat cattctaacc cctaagtcgt
    11761 aaaatttctt taaattttct aatttacctt caagtactgc accttcctca atagataaaa
    11821 gagctgtaag tttaccttct gattggtttt tcataatttc ttcatagtta gtagctaaag
    11881 ctatttcatc actatttttt ttcatttcct catgaaattt atttgccata ctcatgcaat
    11941 aatcaaaagg atgttttact tcttcagtat caacaaaaag agcaaatgtt tgagctaatg
    12001 aatccccctt tttcaatctg tctatatcaa cagaatattt attagactta agttcacttg
    12061 tttctacatt ttccattaat tttgctatag tatcacaatg taaatcaata aatttcatat
    12121 ttataccccc tgtatatttt atatttatat ctaaaaatag aaatagttgt ccttatagac
    12181 aactattgac aactattttt taatattctt tttaatatta cttaatctta ttcttctttt
    12241 aagttaggta ttactttttt aacttcttca atttgatgcc atgttttatc tatattattt
    12301 tcactaatat atttttttaa atcagttaaa gatgtaaaat ctttaactga taaattgtca
    12361 tcatcatctg caaatcttaa aataaacatg agactccctt actttccaca acatttctta
    12421 tatttttttc cgcttccaca tgggcatgaa tcatttcttc ctatttttgc ttctttaact
    12481 attgttttag acttcttata agcctttatt atttcttttc tttggtctgc tggtagtata
    12541 tcttcccaac cttgtaaatt atatagccaa tgagcctcaa cagttaccat attgttatat
    12601 aatttttccc aatctatttt tatagaaact tctttatcag cttctacttc ttctaaaact
    12661 atatcttctt ttaagctttc acttattcca tctatgaatc ccatgaaaaa ttcattagta
    12721 gttccaaatt tttcagccaa ttcacttact ttaccttcaa caacatccat tttattattt
    12781 aatatttcat tgtatattcc agcttcaact ttcaaatatt cttcccagaa ttttacttct
    12841 gcttcttgac tttcatgatt atcacttaaa tttctccatt cagtatataa actcatactt
    12901 tttcctccta agtataccta tatttttata gtttttatag tataaaatct tttagttaga
    12961 ttggaagttt cttctttttg attataccat attctattta aagttaactc ttatattata
    13021 tcataattaa acaattcttt taatactttt atagcatact cttggtttat aaaataaggg
    13081 cttgtcgcaa ttgttattac tttggctttt ttaattaagt tttttatctt gtaaaatttg
    13141 agttcaaatg gtatatagtc catatcactt gaaaatatat ccaaatctat gtcaagaaca
    13201 aactctgatt ctacttctaa atcaaatcca tatagactat ctattattat taattctgaa
    13261 aatatgttat attttaaagc aggttttata aaacttccca catttaaaac ttcatttgta
    13321 tatctaaata catcttttag attatttaca tctacatcat agttctcagg ttctctagta
    13381 tccttatgtt ggtctacatg cactaacttg catccttttg taaactcacc ctttaacaaa
    13441 gactttgccc aaaaataaaa tgcatggtta tgattgtcaa acacatatat ctttttattt
    13501 cccaggacat attcgaccat attctccaat ccttttgctt ttatttcctt attttcatct
    13561 acttcattaa atactacctc attgcctaac cttacatcat ttaatgtacc atttataagt
    13621 tttggaacgt atatgctctt attttctctt ttatcatatg aaaatacatt atttcctact
    13681 ggtttttcta tataaaatcc actatattca taattttcca taaaacttta tttcctccat
    13741 tgttatataa atatataagt
//
LOCUS       DAEOSP010000103        13705 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.103, whole genome shotgun sequence.
ACCESSION   DAEOSP010000103 DAEOSP010000000
VERSION     DAEOSP010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13705
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.103"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            139..1587
                     /locus_tag="KRH54_002273"
     CDS             139..1587
                     /locus_tag="KRH54_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG8579930.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERYNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            1613..4003
                     /locus_tag="KRH54_002274"
     CDS             1613..4003
                     /locus_tag="KRH54_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG8579931.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDYFD
                     LSKGITSEHSRILDLNEGILKRSFIWENSCGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPTGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            4005..4676
                     /gene="pgmB"
                     /locus_tag="KRH54_002275"
     CDS             4005..4676
                     /gene="pgmB"
                     /locus_tag="KRH54_002275"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG8579932.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLKRGKPYPDIFIEGSKILNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNCNTLLQAWSK
                     KINLD"
     gene            4715..6079
                     /locus_tag="KRH54_002276"
     CDS             4715..6079
                     /locus_tag="KRH54_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG8579933.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKKNSILN
                     NIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYFMP
                     IILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYVASVIP
                     ALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLASI
                     INLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCANL
                     AQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGAGI
                     AGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFGVE
                     KNVK"
     gene            6426..7268
                     /locus_tag="KRH54_002277"
     CDS             6426..7268
                     /locus_tag="KRH54_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG8579934.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            7413..9341
                     /locus_tag="KRH54_002278"
     CDS             7413..9341
                     /locus_tag="KRH54_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG8579935.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVYAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            9402..10829
                     /gene="ascB"
                     /locus_tag="KRH54_002279"
     CDS             9402..10829
                     /gene="ascB"
                     /locus_tag="KRH54_002279"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBG8579936.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            11276..12052
                     /locus_tag="KRH54_002280"
     CDS             11276..12052
                     /locus_tag="KRH54_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG8579937.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            12068..12433
                     /locus_tag="KRH54_002281"
     CDS             12068..12433
                     /locus_tag="KRH54_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG8579938.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(12599..13348)
                     /locus_tag="KRH54_002282"
     CDS             complement(12599..13348)
                     /locus_tag="KRH54_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBG8579939.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
BASE COUNT         5353 a         1467 c         2438 g         4447 t
ORIGIN      
        1 acaatattaa ttatagtgag taaaaaatat aattggattt ttaaattgaa ttatttaaaa
       61 ggtatagtat gaaaaaaatc ttataactaa atatgagata gaaattataa aactttatat
      121 tgtgaaagag gtagatgcat gaaattaggg aaagattttt tatggggagg ggcaatttca
      181 gccaatcaaa gtgaaggatt ttatttagat ggaggaaaag gtttatctca gtttgataaa
      241 ctcccattaa atgataagag attaaaagat gtatgtttag atgaggataa tattttaaat
      301 gaaaataatc aatactatcc aagtcatata gggattaaat tttgtgaaaa ttacaaagaa
      361 gatatagcaa tgttagcaga aataggtttt aaatgtttta gattttctat atcttggtca
      421 agaatattcc caacaggaga agaaagtgaa ccaaatgaaa agggattaca actttatgat
      481 aatataataa aagaattaaa aaaatataat atagagccat taataacgat aagccacttt
      541 gatatacctt attctctagt tgaaaaatat tctgggtggg ctgatagaag agtggttaat
      601 ttatatgtaa aatatgctga aattttgatg aaaagatata aagatgatgt caaatactgg
      661 attcctttta acgaaatgaa tatgataatg catattccat atataggtgc tgggttaact
      721 tttatagctg atgaaaatca acttcagaaa aaatatcaag cagcacacca tcaattatta
      781 gctaatgcaa aaactataga aataggaagg tatataaata aagaatttaa gtttgggtgt
      841 atgttagctg ctggaaaaac atatccatac acatgtaaac cagaagatgt atttgcaact
      901 ttagagcatg aaaggtataa tttgtttttt tctgatgttc aagttagggg tgtgtatcca
      961 agctttatga attattattt aaaatcaaat caaataaaat taataataaa agaggaagat
     1021 ttatcaacac ttaaggataa tactgtagat tttgtatctt ttagctatta ttctagtgct
     1081 tgtagttcag ctagagctga aggtttagaa aaatcaaagg caaacggacc agaaaccata
     1141 aaaaatccat atcttccaaa atcaaatagt gtatggcaaa acgaccctgt agggttaaga
     1201 ataactatga atcagttata tgaaaggtat cagttacctt tatttatagt agaaaatgga
     1261 ctgggtactc aagatattgt gaatgaagat ggaaaaataa ttgatgatta tcgtatagat
     1321 tacatgaaag aacatattaa aaatatgatt cttggagtaa atgaagatgg aatagagctt
     1381 ataggatatc ttatgtgggg atgtatagat gtaaccagtg taagtgaagg aaagatgaca
     1441 aaacgatatg gcataattca tgttgattct gatgactatg gaagaggctc atttaaaaga
     1501 actaaaaaac aaagtgcttt ttggtataaa aaagtaatag aaacagatgg agaaatttta
     1561 tttaatgaca tggaaactca tgcataattc attttaagaa aggagtgtta ttatgagttt
     1621 aatatttact actaaggacc aacttataga gcaagggttt agtaaggata caataaaatc
     1681 caatgaaacg ctgtttacac tagctaatgg acatttagga gttagaggga atattgaaga
     1741 gtctgatttt aataatgagt atgtagataa tatgggtaca tatgtaaatg gattttatga
     1801 agaatctcca attacatatg gagaaagcgc ttatggctat gcaaaacaaa atcagactat
     1861 ctgtaagtta cctaatgctc atatagtaaa tttctctatt gaaggagatt attttgattt
     1921 atcaaaaggt attacaagtg aacattctag gatactagat ttaaatgaag ggattcttaa
     1981 acgttcattt atatgggaaa actcttgtgg tagaaaagtt aaagtatcta tagaaagatt
     2041 agtatcacaa gatatagaag aattaatgat gatttcatat acaataacac cattgaattt
     2101 taatggaaaa ataaaattta aaaataaaat ggaaaatagt atggagaagt ttaattgtga
     2161 aaaagtagat gacccaagag tggcatctat aaataagaag caatttaaaa ttaatgtaaa
     2221 aaaatataac aataagtatt ttatgaattt agttacagaa agaagtagat tagggttatg
     2281 gtgtgggttt gattcatata ttacttgtgg aaatattaca aatgagacct ataatgtttc
     2341 agatgaatgt attgagcgag aaatagaagt ctatgccaaa aaagatgaaa ggttaaattt
     2401 agaaattgtt gtaggctata gtagagtata ttctgattta aatctaaatg aagaacgttt
     2461 aaaatcatta gaatgtctgg agtcgttaaa atgtttagaa tcagaagcac atttaaaatc
     2521 attagaattg gttttaagag aagcatgtaa tattggattt gataaagtca aaaatattca
     2581 gagaaagaaa atgaatgagt tttggacaac ctctgatgta gagattgaag gcgataaaac
     2641 tcttcaactg gggataaaaa ccaatttatt tcatatttat caatcagcag gaagagatgg
     2701 aaagactaat atatcagcaa aaggtcttac aggtgatggc tatgaggggc attatttctg
     2761 ggatacagaa atgtatatat taccattttt tatatatact caaccatcca ttgctagagc
     2821 tttattagat tatagatact caatcttaga tgcatcaaga gaaagagcta gagaacttgg
     2881 aataaataaa ggttgcttat attcatggag aactataaat ggtaatgaat gcagtgcata
     2941 ttatccaaca ggaacagcac agtttcatat aaatgcagat atagcttatg gtattagaac
     3001 gtattttgaa gcaactaatg atgaagaatt tatgatgcaa aatggcttag aaattcttat
     3061 agaaacagca agattttggg ttgaatttgg agattatata gaatcaaaaa ataataaatt
     3121 ctgtataaat ggagtaacag gaccagatga atatacagca atagtaaaca ataattacta
     3181 cactaattta atggctaagt ataatttaat atatgcagta cagatggtag ataaatttaa
     3241 gcataattta ttttcaaatg aagtatttag aaaagttaaa ttacaagaat atgaagtgaa
     3301 aaactggata aatgccgctc aaaatatgta tttaccatat gatgaagaga agaaaataag
     3361 caaacaagac gattcatttt tagaaaaatc agtatgggat tttaaaaata caccaaaaga
     3421 aaaatatcca ttacttctcc attatcatcc attaacaata tatagatatc aagtaaataa
     3481 acaagcagat actgtacttt cagcgttgct tttccctgat gagtttagct tagaacaaaa
     3541 gaagagagat tttgaatact atgaaaaaat aactactcat gattcatctt tgtcaaggtc
     3601 aatatttgga atgatggcaa gtgaaattgg aaatcatgaa aaagcttata actattttat
     3661 ggacacagca ttaatggata ttaataacct acaaaaaaat acaagtgatg gaatacatgc
     3721 tgctaatatg ggtggaacat ggatgagttt agtatatgga tttgcaggaa tgaaaataaa
     3781 aaatgataaa ttaagttttg aaccaaggat acctaaacac tggaaaaata ttaaatttaa
     3841 tatattattt agagataatt tagtttctgt aaatatagaa gaatataaat cttattataa
     3901 gttaatcaaa ggtagaaata taaccatatt acatgataat aaagaaataa aattatcaaa
     3961 gaacataata gaagttaata attgttttaa agggagtaat taatatgaaa gcagtcttat
     4021 ttgatcttga tggagttata acagatactg ctaagtatca ttttgaagct tggcatgaga
     4081 tagcaaaatc gctagatata gaaatagatg aaacttttaa tgaaaaatta aaaggcataa
     4141 gcagggaaga atcattagat aaaatattgt tatctgctaa taaagtagat agttatacta
     4201 aagaggaaaa gttaaaggtg tgtgaagaaa aaaataacat ttatataaag ttaataaact
     4261 cattaacata taaagatata ttaccaggga taaatgagct tataaaagaa cttaaaaaaa
     4321 ataatgtgaa attgggaata gcatctgcta gtaagaatgc acctaggata ttagaatctt
     4381 tgggaataaa agatgaattt gattgtatag taaatccatc tttattaaaa aggggaaaac
     4441 catatcctga tatttttatt gaagggagca agattctaaa tttaaaacaa tatgattgtg
     4501 taggaataga agattcttat tcaggtatta aagctataaa tgctgctaat atgacatcaa
     4561 ttggtgtggg gaaaaaagaa gtactaggaa aatgtgacca tattgtagaa gatactagct
     4621 tattaaattg caatacacta ttacaagctt ggagtaaaaa gattaatttg gattaaaatt
     4681 atattaaaaa ttattttaaa agagaggata aggtatgaag aataaatatg aagaagtagc
     4741 tcaattgata attgatggcc ttggaggaaa ggataatata attgacttaa ctcattgtat
     4801 gacacgatta aggttcatct taaaagatga aaagaaagtc aatgcagata aattaaaatc
     4861 tatagataag gtagttggag taaactcaac ttcaactcaa tatcaggtta taataggtaa
     4921 tgaggtaggt gttgtattta atgcaataat aaataagggg ataaatacca caggagatat
     4981 taaaagcgat ataaaaaata aaaaaaatag tattttaaat aatataatag atacaataac
     5041 tggttgtatg acacctatga taccagccct tacagcatct gggatgataa aagttatatt
     5101 atctctttgc accactttta attggctatc atcaaattca agtacatata gagtattaga
     5161 tataatagga gatgtagcat tttactttat gcctataata cttgcaataa atgcagctaa
     5221 aaaatttaat gttaatacat caatagcagt tatagtagta ggtgtgtttt tacatccaaa
     5281 tttctctgca tgggtatcaa gtggggaccc tatatcattt ataggagtac caattcaagg
     5341 tgttatatat gtagcatctg ttatccctgc acttttaaca gtatggatga tgtcatatat
     5401 agaaaaattc attgataaat taacacctag catgttaaaa acaattttaa atccaacttt
     5461 agtattatta ataagtgcgc caatagcatt aatagtaatt ggaccaatag gaaatttctt
     5521 aggagaggga ttagctagta taataaattt actacaagga aggttaggat ttataatggt
     5581 ttgtttatta gcagctgcaa tgccatttat cgttatgaca gggatgcatc atgcattgac
     5641 tcctatattc atatcagcat ttgcaactac aggacaagaa tcgctaatat taatatctca
     5701 agtttgtgct aatttagcgc aaggtggcgc aacattagct gtagcattaa gaagcaaaga
     5761 tagtaagatg aaacagttag catcagctgc atggatatca gctatcatgg gtataactga
     5821 accagctctt tatggagtta cattgaaact taaaaaacct gctatgttag catctatagg
     5881 agcaggaatc gcagggttat ttgcaggaat tactcatgtg actttatatg ttccacaaaa
     5941 tagtataatg gcagtacttg gattatcagg ggaaaagggg atttcaaata tagtaaatgg
     6001 acttatagtt atatttatag cattggttgc tccatttatt atgacatatg tttttggagt
     6061 tgaaaaaaat gttaaataaa gtacagataa tgtagtagag gaagtataat attaactatt
     6121 tacattaata gaatttttat tattattgac tataacttgt tttgtattta tattgatgca
     6181 aagaaataag ttaatattaa taagaataat tattagaact gacaaaaagc aacataaaag
     6241 attgatgaag tatatggaaa taaaaagttg acaaaatatg tctgatgttt tatcatatta
     6301 attggaatta ggtagtaatt gtttaagtac aagctaagct tattcattaa taatagcagt
     6361 atcctaggaa ggagtatgcc cattattatg gataagcttt ttttatgctt aaaaggagga
     6421 taatcatgat aattaataag atattaaaca ataatgttgt aatcacatta gatgataatg
     6481 atgaagaagt tatcgttatg ggtaaaggaa ttggttatca aaaatcaaaa ggcaatctaa
     6541 tagataaaac taaagtaaat aaagtattta gaatatcaaa taaagaaata tcaaacaaac
     6601 tacaagaatt actcaataat attcctatag aacacatgaa attatcaagt gaaattatag
     6661 aatacgcaca aataaagtta aacaagaagc taaatgaaag tatttacata tccttaagtg
     6721 accatacata ttctgcaatt caaaggatga aagaaggaat aaatgtaaaa aatgcaatcc
     6781 tttgggaaat taaaagattt tataaagaag agtttgaaat tggaatgaaa gcactagata
     6841 ttattgaaaa caaaacaggt ataaaattac cagaagatga ggctggattt atagcctttc
     6901 atatagtgaa tgctcagtta agtgaaggtc atactttggc atctgatatt acgaagttaa
     6961 tacaagaagt acttagcata gttaggtatc attttaggat agaatttcat gaagaatcag
     7021 tgttttatta tagatttata atgcatttaa agttttttgc gcaaagattg cttttagata
     7081 gtgcacatga aggtgagaca gacaaagaat tactaagcat tattaaatct aaatataata
     7141 aagagtttga atgtgtagtt aaaattaaaa atttcattaa aaaacaatat aactacatat
     7201 taacagatga tgagcttata tatttaacaa tacatttagc taaagtagtt aaagactcaa
     7261 atatgtaagt gaaataattt ttcagtagta tattaagaat gattgaaagg agtgatacta
     7321 acaaaattat acttatggtt ggatggtaac attgagttgg ccaaaccttc aaatttcaaa
     7381 aaattaattt aaacgaaata tggaggagaa aaatgggaaa atacaaacaa ttagcacaag
     7441 agatagtaaa caatgttggt ggaaaagaaa atattagtgg gttagtacac tgtattacaa
     7501 gattacgttt tactttaaaa gatgaaagta tagcaaatga caatgtatta aaaaacatgg
     7561 aaggtattgt tactgtaatg aaaagtggag gacaatatca agtagttata ggaaatcatg
     7621 ttgaagctgt ctataaagat gtagttgaaa taattggttt agatgattca agtacaagtt
     7681 cagaaactaa aaaatcaggt aatatactag ataaaggtat agatataata tcaggaattt
     7741 ttcagccagt gttaggaata atggcagctt gtggtatgtt gaaaggattt aatgcattat
     7801 ttgtagcaat gggattatat agtgatgtat ctggtggtta tatggtaata aatgcagcag
     7861 gagatgcatt atttacattc cttcctttgt tcttaggata tacttcagct aagaagtttg
     7921 gattaaaacc aatgttggga ttagcactag gagctgcagt atgttatcct gctatacaag
     7981 gtagcagtat atcagcagga gcagatgtga tgtacacttt atttaaagga acaatgtttg
     8041 catcaccagt atatattgat ttctttggac taccaattgt atcaattgat tatactggaa
     8101 cagtcgtacc aattatattt atagtgtatt ttgcttctag gtgtgaaaaa ttatttaata
     8161 agtgtgttcc aaatttagtt aagtttttct ttgtgccaat gcttacatta ttaataacag
     8221 tgcctgttgc acttatagtt ataggcccta tagcaacatt tggttctaca cttatttcac
     8281 aaattgtaat ttcaataaga gattttagtc cactattggc aggtgctata gtaggattta
     8341 cttggcaaat tcttgttatt tttggtatgc actggggatt tattcctgta tatataaaca
     8401 atgttatgac tttaggatat gataatgtta tgatgccatt ctttgcctgt acatttgcaa
     8461 gttcggcagt tgtgttggca atatttttca agactaaaga taaaaaacta aaagagatgg
     8521 caataccaaa ctttatatct ggtatatttg gagttacaga gccagcaatt tatggaatat
     8581 tattgccttt aaagaaacca tttataataa gttgtattgc tagtggtatt ggaggcgctt
     8641 tctatggatt ctttaatctt agaaaattta taactggagg aatgggaata tttgaacttc
     8701 cagcaatgat tgaaccagat gggggaatgg gtaacttaat agtagctctt tctggtatag
     8761 ctatatcaat ggtcgtagct tttgtattaa ctatgatttt atataaagat gagaaagtag
     8821 aagagtctaa aaaagtaaga aataagggta aagaagaaat aaaagaagtc aagtctacta
     8881 aattagaaag agaaatagta acaagtccta taaaaggtga agtattaaaa ttaagtgata
     8941 tagaggatgc agcatttgca tctggtgtac taggtcaagg ggtggcaata atacctagtg
     9001 atggaaaagt agttgctcca gtagatgggg tagttacaac cttatttcct agtctacatg
     9061 ctatagggat tctttctgat acaggtgttg aagtgcttat tcatattgga ttaaatacta
     9121 ttcaattaga gggaagaggt tttgaggcat gtataaaaca aggtgataga attacaaaag
     9181 gtcaaactat attaaacttt gatgtttatg caataaaaga gttggggtat agtactgtaa
     9241 ctccaattgt tattacaaat tcatcacaat ttttagatgt agtagaaaca gaaagtaaaa
     9301 atattgaatt agaagacaat ctaattacag tcttatttta gtaaagattg aggtattagc
     9361 ataatctagt gctttggtta taattttagg aggctttgac tatgagtttt aagaatgatt
     9421 ttttatgggg aggagctact gcggccaatc aatgtgaagg tggatacaat gaagacgaaa
     9481 gaggattggc aaatgtagat gtatgtccaa caggaaaaga tagaacagca gttattacag
     9541 ggaaactaaa aatgtttgat tttgatgatg agcattatta tccagctaaa actggtattg
     9601 atatgtatca taattataaa gaagatataa agttatttgc tgaaatggga tttaaagtat
     9661 atagaatgag cattgcatgg agtcgaatat ttccaaaagg tgatgaagaa acaccaaatg
     9721 aaaagggact tcaattttat gaaaatatat ttaaggagtg taaaaaatat aaaatagagc
     9781 ctttagtcac tattacacac tttgattgtc caatgcattt agttaaaaag tatggtgcat
     9841 ggcgtaatcg aaaaatgata ggtttctatg agaaattatg caatgttatc tttagaagat
     9901 ataaaggatt agtgaagtac tggctaactt tcaatgaaat aaatatgatt ttacatgctc
     9961 catttatggg tgctggtatt tgctttgaag aaggtgaaga tgttgaaaag ataaaatatc
    10021 aagctgccca tcatgagctt gtagctagtg caattgcaac taaaatagca catgaagttg
    10081 attcaaataa cttaattgga tgtatgtttg cagctggaag tgtatatcct tatagttgta
    10141 atcctaatga tgtatgggaa gctacaaagt tagatagaga aaattacttc tttgtggatg
    10201 tacaatctaa aggtaaatat cctaactatg cgttaaagta tatggagcaa aaaggtatta
    10261 caccagaaat ggaacctggt gatattgaat tattgaataa atatacagta gattttattt
    10321 cattttcata ttacaatagc cgttgtgtta gaaccgatga aaatgcagat gatatggctg
    10381 aaggaaacat atttacatct gctaaaaatc catatctttc atacagtcaa tggggatggc
    10441 caattgaccc attaggtttg agaattacac taaatcatgt atatgataga tatgaaaagc
    10501 cactatttat tgtagaaaat ggattaggtg ctaaagatat agctgatgaa aatggttatg
    10561 tagaagatga ttacagaatt gattatctaa gagaacatat aaaagctatg catgatgcag
    10621 tttctataga tggggtcgat ttattaggtt atactacatg ggcacctatt gatttagtaa
    10681 gtgctggtac aggtgaaatg gaaaagcgtt atggatttat atatgtagat agagacaata
    10741 gtggtaatgg aagtctaaga agaatgaaaa agaaaagttt tgaatggtat aagaaagtaa
    10801 ttgcttcaaa tggtgaagat ttatattaga caagaaatct atgttaaata agaaatctat
    10861 attagacaag aaatctatgt taaataagaa ctttgtatta aataagaaga gtataaaaat
    10921 taataataag tataattttt aactttttta actatagaaa atcaatatag aattatttta
    10981 cattgtattg atgcaatagt atgattcaca aataaaattt tagaaaataa aattgttaag
    11041 aagtatggga agtaagtgca atttactttg taataaaaga tgaaaaaggc aagcttaggt
    11101 tttaagcttg tcttttttat tatgtataaa ctatgatatt ttaaaattct atgaaaaatt
    11161 tatttaatag agaaattaaa aatttaatgg taatcaaatt ctttaccaat gaatataaaa
    11221 aatgtctacg tgttataata gtttaaacta ataaacgaaa ggtagatgaa tgaatatgaa
    11281 ataccaacat ataatagaag ttgacaaaga actttgcatt ggatgtgggc tttgtaaaaa
    11341 tgattgtcca gtaaacaata taattataga aaataaaaag tcagtaatta aaaaacaaga
    11401 ttgtctaatg tgtggacatt gtgcagcaat atgtcccaca aaagctatta ccttgacagg
    11461 atttgatgaa cctcctatag aacttacaaa taagccaaag ctagattcag atgaattact
    11521 aatggcaatt aaatctagaa gaagtatacg taaatttaaa gataaagaag tttcttcaga
    11581 aattataaag caaattattg aggcaggtag gtatacccct agtgctaaaa attcacaaga
    11641 tgtatcatat attgttttag acaataaaaa gtcaatttat gaaaacgaag cagtgaaatt
    11701 ttttagaaaa ataaaaccta ttgcaaatat agctattaaa tattcaaaag aagttgagat
    11761 tgacgataac tttttcttta agcatgctcc tattgcaata atgataatta caaaagataa
    11821 gattagtggt tcacttgcag catcaaatat ggcattaatg gcagagtctt atggtctagg
    11881 tgttttattt agtggatatt tttcagatgt agcaaataat tcgccaaaat taaaaaagct
    11941 tttaggtcta aaacgtagta atcatgttct tacaacatta gtaattggat acccagatgt
    12001 taaatataga cgtactgcac agaaagaagt agcaactgtt agatacttat aagagagggt
    12061 ggattttatg gaaaaagaat gtcaacaaag aatgcaagaa ataacgtcta aatttaaaga
    12121 atgtcgcaaa gctatctctg ctatgggtga tgagactaga caattgattt taattgctct
    12181 tttagagagt gattttaatg gtatacgagt gggagaaatc acaaaaaaaa cacatctttc
    12241 tcgtcctgct gtatctcatc atcttaagat attaaaagaa gcagagattg ttaatttgcg
    12301 tagagaagga acgaaaaatt attattatct tgattcaaaa aaatcacaat ggaagtcact
    12361 aacagagcta ataaatctta tatttgttgg aatacaacat attagtgaaa atgatataag
    12421 aaaaggtgat taaattatac ttgatttaat acaaaaagat tgatttagga actaaaaaat
    12481 tcactttgag acactatctt ttgaactata cccattttag gagacggtta aaatatgtaa
    12541 aactgtataa aacaatgggt atagttcatt ttataatggt ttaaatatta agaatatatt
    12601 attctaaata gtatctttct ctgttttgag ttttattttt atactcaata gcttcagtgg
    12661 ggcaaccaca aatacatgcc atacaatgag tacagttatt tttccaaact ggttttttgt
    12721 tttttaaatt aatattattt aaaggacata gctctacaca ttttccacag ccaatgcatt
    12781 tatcagtata atgaaatcct ttggcactaa taattgtctt ataaaaaact gtatttatta
    12841 taccactttt aattgtacct cctaagccac taggagtaat agtttcaaat tcattttcat
    12901 tgcgtatatc agaaataatc ctatatattt gtttatctgc ttctttaatc atttgttttg
    12961 atgtttcttt gtctgttgta gaaaataaag ctatataatt ctctggcatt ttaatttctg
    13021 ccataccttt taattgccat tccttgtatt tacatagttt ttgaatatag tttatggatt
    13081 ttgctgtatc tccaccacaa gtcattacaa agtaaactct gttgcttcct agaaattttg
    13141 tttcttttat aaaatccgta actactattg gcaatctcca tgcataagta ggacatacaa
    13201 atatgaatgg tttatctgct gatattaatt catctgtttt ttcctctttt ataagttgat
    13261 ttagtgatat cagttcatca tttaattctt gtgcaatttt acgagccaca tatcggctat
    13321 taccagttcc gctaaaatat aaaatcataa taatatctcc ctaactattt tttatttatt
    13381 atagtaattt attgatatat tctattcaaa tgtatgtaaa atttagtagt taagaaaatt
    13441 ataacacatt agtaattttt atacttattg gtaaagaatt tgattaccat taaatttagc
    13501 aacaagacat caactggact tgtatatgag aagggataat aatttgctta tgaaagatta
    13561 taaatataaa ggttatttta aaggtgtaga aatatttgat aatgtaaatg accttaactt
    13621 aaatacttta acaacagtta gtagatttgt gcttgttttt ttgtttacga taacttaagg
    13681 aaatatgtta cctgaataat aaatt
//
LOCUS       DAEOSP010000104        13587 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.104, whole genome shotgun sequence.
ACCESSION   DAEOSP010000104 DAEOSP010000000
VERSION     DAEOSP010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13587
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.104"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(21..734)
                     /locus_tag="KRH54_002283"
     CDS             complement(21..734)
                     /locus_tag="KRH54_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBG8579940.1"
                     /translation="MGKVAKILLSLIVIVLVLVGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            1026..2690
                     /locus_tag="KRH54_002284"
     CDS             1026..2690
                     /locus_tag="KRH54_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBG8579941.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(2797..3696)
                     /locus_tag="KRH54_002285"
     CDS             complement(2797..3696)
                     /locus_tag="KRH54_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBG8579942.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(3723..4361)
                     /locus_tag="KRH54_002286"
     CDS             complement(3723..4361)
                     /locus_tag="KRH54_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG8579943.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(4702..5700)
                     /locus_tag="KRH54_002287"
     CDS             complement(4702..5700)
                     /locus_tag="KRH54_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HBG8579944.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(5798..6373)
                     /locus_tag="KRH54_002288"
     CDS             complement(5798..6373)
                     /locus_tag="KRH54_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579945.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            6689..6952
                     /locus_tag="KRH54_002289"
     CDS             6689..6952
                     /locus_tag="KRH54_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579946.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(7389..7766)
                     /locus_tag="KRH54_002290"
     CDS             complement(7389..7766)
                     /locus_tag="KRH54_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8579947.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
     gene            8107..9219
                     /locus_tag="KRH54_002291"
     CDS             8107..9219
                     /locus_tag="KRH54_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG8579948.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(9448..10263)
                     /locus_tag="KRH54_002292"
     CDS             complement(9448..10263)
                     /locus_tag="KRH54_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8579949.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTIELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIITGCSHSGICNIVEYAKKVCRDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
     gene            complement(10560..12002)
                     /locus_tag="KRH54_002293"
     CDS             complement(10560..12002)
                     /locus_tag="KRH54_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG8579950.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFART
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            complement(12129..12515)
                     /locus_tag="KRH54_002294"
     CDS             complement(12129..12515)
                     /locus_tag="KRH54_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBG8579951.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            complement(12552..12941)
                     /locus_tag="KRH54_002295"
     CDS             complement(12552..12941)
                     /locus_tag="KRH54_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBG8579952.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            complement(12973..13509)
                     /locus_tag="KRH54_002296"
     CDS             complement(12973..13509)
                     /locus_tag="KRH54_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG8579953.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
BASE COUNT         4730 a         2180 c         1468 g         5209 t
ORIGIN      
        1 aatataagtt ttctatattt ttaaccaaca gatgttttct tcaaattaat cagatgattt
       61 agtatatctt gaacctttcc agagtccttt aaatatccta atgccccatt tatttgtgat
      121 tttaaatcta tttctacttc catatccaaa tcaacatttc ctttttttat agatatattt
      181 ttaagtgtta ttggataaag ataacttttt tccaaagtaa tcttatttcc attaacctca
      241 aatggaactt ttttattgta gttacttaat attttttcta gcattttatc acttagttta
      301 aattttccaa ttttcacatt ctcaagactt acaactaagt ttccatcctt taaagtaggt
      361 ctcaattcaa gttcatattg actatttacc aaaccaaata ccttatatgg tcctgctacc
      421 tttattttac catttttcat ttcaacaaaa ttattttcaa gttcattaat tccatgcttc
      481 tgcatcaatg tatatattaa atttttaaac tcttcctctg aaacagatat ttttccaacc
      541 attctaagtg gattttttac tacctccata gaatttatat atgagccttt tgataaatat
      601 tcttcatcta tgattttatt attttgtgaa ctaacatcat atctttcttt tggtgtcaag
      661 atatatacaa caattcctac aagtactaaa acaataacaa ttaaacttaa caaaatcttt
      721 gcaacctttc ccataatcgt ctcctttaca aatctgtcat cagatttaaa ataaatttag
      781 ataactaact tcacataata ttcttaacaa tatacatact ataatacagt cttttaaaaa
      841 ttaattcaac caatctaatg ttttgtaaat aaactgtaaa aacatgtttt tagtataaaa
      901 gttaatattt tgcataatac tcttaattac acctttattt ttttctaatc ttggattaaa
      961 attaatatta tgcaatacag tgtagtgttc tgtattataa tcaaattaaa taggaggtat
     1021 agaatatgtc aaatgaaacg aactcatcta actttataaa gaatattatt ataaacgact
     1081 tagaaacagg aaaacatgac agtatcataa ctcgttttcc acctgagcca aacggatatt
     1141 tacatattgg tcatgcaaaa agtatatgtc ttaattttgg attagctaag gagtttaatg
     1201 gaaaagctaa tttaagattt gatgatacaa acccattaaa agaggatgtt gaatatgtag
     1261 agtccataaa agaagatgta aagtggttag gatttgattg gaacgagtta aattttgcat
     1321 ctaactactt tgatgaaatg tataagagag ctttaatact aataaaaaag ggtaaagctt
     1381 atgtatgtga tttgacacaa gaggaaatga gagaatatcg tggtactcta actgaaccag
     1441 gaaaagaaag tcctcataga aacagaacta ttgaagaaaa ccttgattta tttgaaagaa
     1501 tgaaaaatgg tgaatttaaa gatggtgaaa aaacattaag agccaaaata gatatgtctt
     1561 ctccaaatat aaaccttaga gacccaatta tatatagaat atctcattca acacaccata
     1621 atacaggaga taaatggtgc atatatccta tgtatgcttt tgctcatcca attgaagatg
     1681 caattgaagg aataactcac tctatatgta ctttagagtt tgaagaccaa agacctcttt
     1741 atgattggtt tgttaaagaa tgtgagatgg aaaatatacc tagacaaata gaatttgcta
     1801 gacttaatat aaataatact gtcatgagta aaaggaaatt aaaacaactt gtagatgagg
     1861 gtattgttga tggttgggat gaccctcgtg ttcctacaat atccggaatc agacgtaagg
     1921 gatatactgc agaagcattg cgcaatttct gtagtgaaat aggggtatca aaagttaact
     1981 ctacggtaga tagtcaaatg cttgattatt tcctaagaga aaacttacaa ccaaaggctc
     2041 ctcttactat gggagtttta agacctttaa aacttattat aactaattac ccagaagata
     2101 agattgagat gcttgaaata gagaataatg ctaaagatga atctcaagga aagagattag
     2161 ttcctttctc tagagaattg tatatagaac aagatgattt tatggaagaa cctgttaaga
     2221 aatattttag atttttccca ggaaatgaag tccgtttaaa aggtgcttat tttgtaaaat
     2281 gtactgatgt aattaaggat gaaaatggaa atgtagttga aatacatggt acttatgacc
     2341 ctgaaactaa gagtggctct ggctttacag gacgtaaggt aaaatctaca atacattggg
     2401 ttgatgctaa atcagctatc ccatgtgaat ttagattatt tgaaccatta attcttgatg
     2461 atatacctga aaatgaaggt aaacatttct tagaacaaat aaatcctaat tcattggaaa
     2521 tattacaagg ttttgttgaa cctacacaga taaaagatgc taaacctttt gataaattcc
     2581 aatttgttag aaatggattc tttagtatag ataataaata tacaactgat gaaaaatttg
     2641 tatttaatag aatagttcct ctaaaaagtt cttttaaacc aggtaaataa tcttacaatc
     2701 aaaaaatgaa gacttaaaat ataaattact tctactataa ataaacttag ttagaagtaa
     2761 ttttttattt gagtcttcat catttacttt aaatgattaa cttttttcac tacagattcc
     2821 catgacttct attccatctt ttatgcattc tatgttaaga ggaaaatctg gtcctttttc
     2881 tccgtcaata tctgttataa gtgaatcttc acattctata tgcacctttt tagttttaaa
     2941 gtatagtatt tcatttccat tatattgatc taaataatca cctctaagca cacttatcaa
     3001 aactggtatt gattttggta ttggcattcc tttaaaaata attacatcca aatatccatc
     3061 atctatttca gccttatatg ctagatttat atttcctgct gttttgccat taaaaatcaa
     3121 cataagatac atatctccat cataatacat ttcctcagaa gatactttta tcttaaattt
     3181 cctcatatac aatgcttctt ctattccctt tatataataa gaaattcttc caaatgaatt
     3241 ttttaagtct gtgtttatcc tttgtgatac atccgtaaac ataccagcac ttgcaacatt
     3301 tataaagtac ttattattga ctttccctat gtctattttc tttggttttg aatttatgat
     3361 ttcatttata gcttctttta tatcaaatga aatttgaaga gcatttgaaa aatcatttgc
     3421 agttcctgta ggcaaaattc caattggaat ttttatgtct aattctttca ttgcatttac
     3481 tactaaatct acagtaccat caccaccagc tatcaatatg tgttcataag aactatcaac
     3541 ttccttaaat gcattcttta caggttcttt aatagttaat ctatagggta tcaataaata
     3601 atttttttct tgatataatt ctatgatagt gtctaaattt gataatatct ttttttcacc
     3661 tgaattagga ttgtatataa gttttacttt tttcattatt tctccttact cattttattc
     3721 ttttagtatc caaaatcttc atttaaaaaa atcacatgta ttctttctgg tgttaattgt
     3781 ctttttataa gtgtataact acagcatatt cttcctggac ttgaacagtc tatacatata
     3841 ccagtagtct tacatccagt atttgtactt aatcttttac aattggctgg agcagctatt
     3901 gtttttgttc tttttatagc ttcatctaca tctttaacta ttttatttac tcctgctata
     3961 actattactt tatcaggtcc atatatcata gcagcaaccc tattcccatt cccatctaaa
     4021 ttgtaaagtt ctccatcctc tgttatcgca ttagtacttg taaaataagc atctgcagaa
     4081 aatgactttc tatatatttc tttcatttct tctggtttga tatcttttgc atatctatca
     4141 agaaaattat atttttctct tataaaatca atcactcctg tttcaaataa ggtcatagaa
     4201 cctccacatc ctactagaga acctgcttta actatttctt caattttttt aagaagctct
     4261 tctttgttac ttactaaata ccctttcata ttattgtttt ccaaagattt tattgttttt
     4321 tctattcttt tttcattaag ccaatttaaa ttttgatcca ttttaacttc ccccttattt
     4381 ttaatatgta cttaattatt tataaacttt attttcttta taagtttatt ttagtacatc
     4441 taaaaaaaaa tacaatataa aacttaaata gttttacata attttttcca aaataactta
     4501 ctcaatcaaa aaaggaaagt agatgtacta ttctactttc cctatattgt atgtagtatt
     4561 atatattgcc taaaatctat atattaatta tatgtttcat attatatgaa tatcacagtt
     4621 aattaaaatg taatactatc ttctttttaa acatataaat tgtattatta aatttaatat
     4681 atccaacttc aataaatctt attaatttga tgaagaagat gctatagctg cacttgttgc
     4741 tgccattaca catgcaattt gaatagcaac aacaatattt aaaaatatat tattagttat
     4801 agagcctata ttattctcat ttcgtatcaa atctgcataa attgaagcta caatagcaga
     4861 actaatcatt actctattac tttttcctaa agaccaattt ccaaagcctt tttcttcttt
     4921 aagtttattt gaaacatttt ttatctcttc tattatttca tcttctctat aatctaataa
     4981 tgaaattgct cctattattg gatatccata ataattcatt ttacaatcat tttctttaaa
     5041 tgctttttta agttcaatag tctttttaca tttgagcata gttctatcat cactaaataa
     5101 aagaatttgg gataatgctt gtaaatcatt tcctttataa aaaccatttt catttagaaa
     5161 ttcataacat ttttctatgt attctaaatc ttcatctaaa ttttttgaat gaattgcaat
     5221 aagtgctata ttacaatagt catcactaga agttagaaat ggatgttttt tcctcatgta
     5281 atcatatatt attttcattt ttgatataca tgtaagataa tccattttct ctctattttc
     5341 ataaactatt gatgctgtaa gtggtaaata tgtatcgccc caaaactttt cacttttaag
     5401 ttctttgtat atatctaaaa tcatcttaaa actactttct ggatttgatt caaacgaaag
     5461 tagtgttgcc atataaaaca tattatatcc tctaaaatta gagaataatc cagtattttt
     5521 tttaataagc tctattcgtt cttccactaa tctaggactt attctcttgt tcttaagggt
     5581 aaatgcaagt gcacaactat gttgtatcaa tcccatttgc caagaacctt taacttcatt
     5641 taatgctttg taattttcta ttaataagtt tacttttcca tttataaacg attgttccat
     5701 attgccctcc tagtaatata tctcattgta ctaatagttt tatatttatt atatatatat
     5761 taaatactac aatcaataca tattacttca aagaacatca ttcttcaaca ataatgtagc
     5821 cttttttgta atgtcttccc atattattga ctctatcata gtactctgat tcactttcat
     5881 tactccattt gggcacaaat acatctattg cattagaatt actaaaatat ttactagacc
     5941 ccctatccat aacagttttt aattttccat ctacaataat cttagttcca aaacttaagt
     6001 gattgtttgc tacaaaacca tcttctaaat catctcctgt agcagttatc gctccatatc
     6061 cattttcttg attaagactt gtgtaataac taacttcaaa agttataggt actaacttct
     6121 ttattttaat acctaactga ttttgtagtt tctccttttg tactttcaac ttatactcat
     6181 cactattttt ataatcttcc ataccttgat tatacttgga attaaagctt ttacctgact
     6241 ttactatagt atctatattt actatagatt ctttacaatg atctgcatct atttttgttt
     6301 ctccatttac caataatgga gttaaacata cactacatac aacacatgta atcaatgctt
     6361 ctttaagctt cataaattcc ccccttatta ttttgatata ttagcatatt taatattata
     6421 tgtctacatt tagatttaaa taccacaccc atttgcttta agaagttgtc ttatatttaa
     6481 ttcaccttaa acacttataa atcctctatc ttagaatcat atagaataaa gcttttatct
     6541 ttaaatattt tttattaatc ttttaaaatc taaaactact ttatctaatt ttctcttttt
     6601 tagaatttaa gaattaaaat tctataattt taaaacatat tgaatttatt tttttgagat
     6661 gatataattt tcttaagagg tggttgatat gataaaaaat acaaaacaaa ttacagtaga
     6721 agttaatgaa attttattac aagaaatgtc taagataact aatgacgaaa ataatatttt
     6781 gacagatttg ataaatgaat caattgtaaa ttatattctt gaaaacaaaa aacataatat
     6841 tgaaaataaa gatttagaag aagtgattaa attttacaaa aaagaaaata ctgaaaaacc
     6901 taaaagtgca gcaagagcta tagctgaagc aaaaactcat actctgtatt aatcaaataa
     6961 aaaagttcct aaaaactagg aactttttta tattaataat tattaatata tctcaatatt
     7021 tatctcaaat ctatcaatat aagcatgttt aatacaatct attgtatact tatcttaccc
     7081 tagaaataat caaaacaaaa ataaaattat gccctttata tctaaaacaa ttaatttaca
     7141 tgattaatat acaaaaattc tgacttttaa attcgtatac aatatcaaac taaaataata
     7201 ttaatgacaa acccaatata aattaaattc aaacaggttt gattttacat caaacttatt
     7261 gtattaatat taaataacaa gacaaaaata tttataaaaa tcacaaattt tacataaaac
     7321 tttatatttg attcatttaa ctaattattg taaaaactat ttattttatg ataattaact
     7381 acaccaaatt attcatcttt tttaatatcc ttaacccatt tttcaacaga gttcaattct
     7441 tcttcattta cccccttttt gaacaaggat ttcataaaac tctcaataga acttccatgc
     7501 atattatcta aaaaagtttt tgtttcaaaa tgtaaatact cttcttctcc aataagaact
     7561 gtataatgtg tatgtctatc aatttttctt gcacttaaga attgcttatt tactaatctt
     7621 gataaaagtg ttagtgttgt agtttgcttc caattatata gtttttccat agcttcaatg
     7681 acatcttttg atgctactgt aaaatctgaa ctccaaataa atttcatgac ttttaattct
     7741 gctggtggta tcttagtaat agtcatatga ttttacactc ctctcaaact tctacataat
     7801 taaatactat attaatagac ttttcgagtc aaaggtttta tttaaaatat ctcatccaaa
     7861 tttattaata caaaaaattt atatttttat tcaaaattaa acacacaatc tattaatata
     7921 aagataaata tctattctat aagtataaca gattatcaaa ttaaaattct aatatttgac
     7981 ataatatata cattttttaa ataaatatat acaatgtatt aaaatgtatg caactctatt
     8041 tcaaaattat tttgtgatat atatatgtta taataaatgt ataaaatttt gattggtggg
     8101 ttatatatga atgcatttat tagaaaaaga aataaaaact atgttgttta cctggaattt
     8161 gaagatgaaa agacaggtaa aagaaaacaa aaaaatatgg gtacttttga taaaaaaagg
     8221 gaagcaagta aaagactaaa tgaggtaaaa gaaagcttat ataatgatgg ttttttagta
     8281 ccaagcgaaa ttacagtgtc agaatttcta ttagattttc taaataaata tagtgaaaat
     8341 atatctttag ctacatataa taattatgtt agtatttgta agaattacat taatccatct
     8401 attggaaaat ataaaataca agacttacat ccggttcaca tacaaaacta catagataaa
     8461 ctatcttata aactaaatcc tcaatcaatt aagattcata taaatatatt gaaacttgca
     8521 ataaaaagag cttatagact caaattaata aaagaaaatg ttatggatag tatagaggct
     8581 ccaagatata aaaaatttaa aaatgagatt tatgatagag aacaaatgat aaaattatta
     8641 gatttagcaa aaaatacaga aatggaatta cctattaatt tggctattgg attaggtctt
     8701 agaatttcag aagttttagg tctaacttgg gataatattg attttgaaga aaatacaata
     8761 acagtaaata agataacttc ccgaattaat ggttctgtta ttctcaaaga gccaaagaca
     8821 gaaagctctg ttagaaaaat atcttctccc aaagaattaa tgtctttatt aaaagaatat
     8881 aaaataaaac agaataaaaa tttattaaaa tcttccatta gaaataataa taatttgttg
     8941 tttttcaata agaaatgtga acctattgct gaagatgtta tgagtaaaaa atttaaaaga
     9001 tttttagaaa agaatgaatt acctcatatt agattccatg atttaagaca ctcacatgtt
     9061 actttactta taaattctaa agtacctata aaggttatat ccgagagagt aggacactca
     9121 aatataagta ctactctaag cgtttattct catgtactta aagaaatgga taaagaggct
     9181 tctgacaaaa tttctgaaag tttatttaat gctaattaat attaatttat cttagcttta
     9241 aattagctta gcttattgag atagattcat taatattaaa tgtatctatc tcaaattaat
     9301 aactacttaa ataatctgtt ttattttaat ttatcgcctc agttattata cttttttacg
     9361 aaaaattata ctttacaata aaaaagatat tcccttatag aaaatatctt tttaaccttc
     9421 aacttaaatc ttatatactt tcaaatacta tatacttatg gtcagcccta ctccaacttc
     9481 atttatattc aatatttcat tcatcttcgc ttttacctta aaagaaacac agtgacaagg
     9541 gtacaacatt cttatttctt ctttatccaa atattgtatt gtactatcta acctattatt
     9601 caattcaaaa agatgaaaac cacctagtat accaataact ctatcatctc tacatacctt
     9661 tttagcatac tctactatat tacaaattcc actatgagaa catcctgtaa taataaaaat
     9721 acctttatca gttttacaaa ctatagcaga atcatctaaa acataatcat cttcccacaa
     9781 atctcctatt ttacatttac caattttagc tcttttttca aaatcattta tagaaggtat
     9841 ttcacctaaa aacacacaat tcttgcttag attaaatggc ttatccttag gattatacat
     9901 aaaaatattt ttaatttcct cagcagaaaa tggagctcct atgcttttct ctccatgttt
     9961 tctaggtata aaacagttag gatgtgaaat aagttcaatt gtagataaat catacctatt
    10021 ttctaaaaac tggatacctc ttgtatggtc attatgccca tgagaaaaaa caacatgagt
    10081 taaatttcct aaatctatat ttaatttttc agcgtttgat atgaatacat ctgaataccc
    10141 tgagtcaaac aaaatcctat ttccatcaat ctcaatataa tagcttactg ctggttctcc
    10201 aacaaagtat ctatcaatat aagtattatt gtcgactaaa acttgtatct tcaatctttc
    10261 cataataatc actccttaaa tttttattta ctttaaagta cagaatcttt agtttaatag
    10321 tacgacatca cagaatattt tacaatttaa tatctttaat tttcataaat agaaggaatc
    10381 tatctcaaaa taaagagata agattctttt tcattgctaa aatgataaat atatttggct
    10441 tcataaaaaa aagagtgact tatgaactaa aaagttcatt ttaagacact ctcttttata
    10501 aaatatattt atcatttact tttatagcta tctaaaatct aatagtttca aacttcaatc
    10561 tatacatgct gtatgtttgt ataatttgat atttcagagt taacagtata taaatcatca
    10621 acacttaaat catgtatatc tttatgacca gaagttctag caaaagtctt aagttcttct
    10681 aaacttacat ttaaaaagtt tgccactcta ttagcactgt tttctatatg taatcttttt
    10741 ctcaattcct catcctgagt agctactcct acaggacact taccagaacc acatatacga
    10801 tattgttgac acgccgctgc cataagagca gaacttgcaa tagcaactgc atcagccccc
    10861 atagcaattg ccttagcaaa atcagtagat atacgaagcc cacctgttat aaccaaatct
    10921 atatctaatc catgtgtatc tatgtatttt ctagcacgat aaagagcaaa tattgttgga
    10981 attgaagttg catctttaag caactttgga cttgctccag tagctccacc acgaccatca
    11041 atagttacaa aatcaggttt agcataagct ataaattcca tatctttttc aatatgacct
    11101 gctgaaattt taacacctat tggacgccct tctgaaactt ctctaagttc atctacaagt
    11161 ttctttaaat cctctttgga ttgtatttcc tcaaaacaag atggacttat tacatcttga
    11221 cctacaggct tatttcttac ttttgctatt tcttcagtta ctttttcgcc aggaagatga
    11281 cctcccatac caggttttgt tccttgacca atttttatct ctatagcatc agaattttta
    11341 agattttctt cagttacact atatttattt ggtacatact caaaaatata cttgtatgaa
    11401 gcttccttct cttctggtaa tatacctcct tcaccactac acatagctgt cttattttgg
    11461 gctgcaccct ttgctaaagc tatttttagt tctttagaca aagctccaaa tgacatgtgg
    11521 gaaatataaa ctggattttc tataatcata ggtttctttg ctttttttcc tattattgtt
    11581 gttgtattta cttcatcatg ctcatttaaa ggtaatggat ttaattgagc acccataatt
    11641 aaaatatcat cccaagaaat tactggtagt ttagttctca taggttctat tatagatttt
    11701 cctgtaacag ccatttcgtg aattatatcc atatgtttct caacttcatc atctcttctt
    11761 aaatatgtac ttaagtaatt taaatctccc tctttttcag tatttaattt agattcatct
    11821 tcaactatgt cttctgctat cttttcatct tcttcatagt aaacagtact tatctttcca
    11881 aaataactac gaccttttgt acatacaggg cattcccaat cttcacttaa ttcatcccat
    11941 gttttatcat tcttagattc atcataaata tatccacaaa cactacattt ataaattgac
    12001 atattatttt ctccttatat tatattgaca ttaaacaaaa ttaaaacttg aatttatata
    12061 aatttatagg aggctatttg tatgaaaata catttatagc ctcctttttg actcaattta
    12121 tttaaatatt acagttcagc cttccataat ccatgtaaat tacagtaagc ataggcagaa
    12181 atcactttat catcatcatt caaagcaaat ttaactacag gttctccatc tactgctaga
    12241 catttgcgtt gaccaccttg ttcagtcata agatacaccc atgctatatg atgttcttta
    12301 gtcattggat gagttgtgct acttacttta acagtaacat tatttccatc cacttcaatt
    12361 acaggtacat gtttttcaac tgctgcatct gttgtattag gaacaagttc cttcataggt
    12421 tttccacaac agaaaatagg tacaccacca ctttgtatca ttcctacaag gtttccacaa
    12481 gtctcacata taaaaaattt ttgttcacta cacataaact cacctcatta attattttta
    12541 gaatttgaaa actaacctac ttggctacac cttttacaat tattacaaat ccctttaaaa
    12601 cttatgttat aagatgttat ttttatgtct gtctcctcag atattaattc ttgtaaatta
    12661 ttaagaactt cggattctaa atcgtatacc tctccacata cttcacatat gatatgatag
    12721 tgattatcta ttcgaccatc aaatctatct ggatatcctg gaatactaac ctttcttata
    12781 aagccatgtt ccgttagctg agctaaattt ctatacacag ttcctaagct taaatttgga
    12841 ttatcttttt ttaagttttc atataggtaa tccgcagtag gatgaaccgg attatttaat
    12901 atttcattta aaatcagttc tcgttgttta gaaaatttca tagttaacat tcctttttaa
    12961 taatgataat ttttaataat tttcagcttt tatattaaaa tatgcttttg gatggtcaca
    13021 tactgggcat ttttctggtg cttcagtacc tacatacaca tgaccacagt tttgacattg
    13081 ccaaacaact tcttcatctc ttttaaatat ttttccttca tttaagtttt ctaaaagctt
    13141 taagtatctt tcttcatgct ctttctctat ttttccaaca gcttcaaata aataagctat
    13201 cttgtcaaat ccttcttctc tagcttcttt agcaaatttt gcatacatat cagtccattc
    13261 atagttttca cctgctgctg catcctttaa attttcctct gttgaaggca taccatcatg
    13321 taaaagttta aaccataatt tagcatgttc cttttcattg ttagcagttt cttcaaatat
    13381 agcagctatt tgattataac cttcttttct tgcttttgaa gcgtagtatg tatatttatt
    13441 tcttgcttct gactctcctg caaatcctgc cattaaattc ttttctgttt tagttccttt
    13501 taagttcata attaattcct cccttttaat aatagtaatg attactattt ctatatttat
    13561 atattatatc atttgccaat ttttgtc
//
LOCUS       DAEOSP010000105        13562 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.105, whole genome shotgun sequence.
ACCESSION   DAEOSP010000105 DAEOSP010000000
VERSION     DAEOSP010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13562
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.105"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            39..485
                     /locus_tag="KRH54_002297"
     CDS             39..485
                     /locus_tag="KRH54_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG8579954.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            487..1581
                     /locus_tag="KRH54_002298"
     CDS             487..1581
                     /locus_tag="KRH54_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBG8579955.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVIKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1669..2199
                     /locus_tag="KRH54_002299"
     CDS             1669..2199
                     /locus_tag="KRH54_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG8579956.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYGIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            complement(3134..4510)
                     /locus_tag="KRH54_002300"
     CDS             complement(3134..4510)
                     /locus_tag="KRH54_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579957.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            4779..5951
                     /locus_tag="KRH54_002301"
     CDS             4779..5951
                     /locus_tag="KRH54_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG8579958.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVNSMFERVKLISTVILNLDKFEV"
     gene            5965..6612
                     /locus_tag="KRH54_002302"
     CDS             5965..6612
                     /locus_tag="KRH54_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579959.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            6614..7123
                     /locus_tag="KRH54_002303"
     CDS             6614..7123
                     /locus_tag="KRH54_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBG8579960.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(7487..8599)
                     /locus_tag="KRH54_002304"
     CDS             complement(7487..8599)
                     /locus_tag="KRH54_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG8579961.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            8897..9163
                     /locus_tag="KRH54_002305"
     CDS             8897..9163
                     /locus_tag="KRH54_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HBG8579962.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILPIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            9408..11135
                     /locus_tag="KRH54_002306"
     CDS             9408..11135
                     /locus_tag="KRH54_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8579963.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            11135..12925
                     /locus_tag="KRH54_002307"
     CDS             11135..12925
                     /locus_tag="KRH54_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8579964.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(13087..13395)
                     /locus_tag="KRH54_002308"
     CDS             complement(13087..13395)
                     /locus_tag="KRH54_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579965.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
BASE COUNT         5090 a         1567 c         2235 g         4670 t
ORIGIN      
        1 tagtcaaaca tattataatt atagaaggga aatttaagat ggattttaaa aatttacagt
       61 atgaaagttt gtcatatatt atttgtaact tgcaaaaaaa ctttaaactt tattgtgaaa
      121 aatgtttaaa accatataaa cttacgaatg gtctatattt ctatttaatt tatattaata
      181 aaaataggaa ttgttctttg aatgatgttt ctacagaatt tgaagttgat aaagctcata
      241 caacaagaac tatatctagg ttagaacaag atggatacat agaaaaaatc caaaacccta
      301 atgatagtag agcttttcag ttgagagtaa cagataaggg tgaggaagta ttaggggata
      361 taaaaaacat attttcaaag tgggataatc acataaaaaa agagttctcg gatacagaat
      421 ataaagaact ggtaaagaat ttacatgtgg taaaggatat aaaaacagct gtggaggaag
      481 aataggatgt attgtaattt attaaaacca attaaaattg gaaatttgga attaaaaaat
      541 agagtatctt ttgcacctac aagcatgggt cttaaattgg aagaaaaaat taaaaagttt
      601 tctgatattg ctaagtcagg agtagctctg attactctag gagatgtatc tattagacct
      661 agttttcata aggtagctat aagcctatcg gatgaagatg gagtcattaa atacaagaaa
      721 attgtagatg aaatacacaa ttctggtgca aaggtatcag cacaactatt ttgttcagat
      781 tatgatgtta atttaattaa agatactatg aatatgggaa ttacttcaca tgatgagata
      841 aaaaaaataa tgaatgacgg agtaaaagat tacataacaa atatgcccaa agaagaaatt
      901 aaaaatatta taaatttatt taaagtaaca gcattgaatg caaaaaaagc aggttttgac
      961 atgattcaaa ttcatggtga taggcttgta ggaagttttt catcaagtat atttaataat
     1021 agaaatgatg aatatggtgg aacgtgtgat aatagaagta gatttgcaag cgaaataatt
     1081 agtagtatcc gagatgaagt taaggacatt ccaatagact ataaatttgc aataagacaa
     1141 gaaaatcctc attatggaaa tgcaggagtt ttactttcgg aagttgagta ttttgttaaa
     1201 aaatttgaat ccctaggtgt aaacagtttt catgtaacac ttgcaaatca ttcaaaatta
     1261 gaagatacta taccaaccaa taatcatcct tactttaaag atgaaggctg ttttttatat
     1321 ttagctgatg aggtaaagaa acacacaaat cttccagtat gtggggttgg aaaattgtct
     1381 agcccagatt ttatagaaag tattatttca aataatagag tagatatggt ttctatgtca
     1441 agacaattac tagcagatag taattggtta cagaaagtaa aagatggaag agtagatgag
     1501 attaaaaagt gctgttattg taataagaag tgtgcagatg ctttacaaac acgctctcag
     1561 tttggatgta tactagatta gaatatcctt ataaaaataa ataatttgtt aaggttaata
     1621 tatttttggt gatttagata aatatttaga taaagaggag gagacatcat gaaaagttta
     1681 gttttatatt caagtttaac tggaaatact aaaaagattg cttatactat atatgatgaa
     1741 attcaagaag aaaaagacat taaagatgta aatgaattag ttgattatgg tattgactat
     1801 gaaaattatg atatagtatt cttaggctat tgggtggaca agggaatttg tgataaaaat
     1861 tcaaaacaag tgcttgaaaa tattcacaat aaaaagatag cactatttgg aacaatggga
     1921 gcaagtaaaa aaggaagtta tggagctagt ataatagaaa agatagaatc aattatccca
     1981 aaagataatg aaatactagg ttcattcata tgtcaaggta agatagcaga aggattgaaa
     2041 gcaaaatata aagagatgtt aaaactttct cctgacaacg aacatattcg ccaacaatta
     2101 aataaccatg aggaatctca aagtcatcca gatgaacaag aaatatatga agccagtatg
     2161 tttgcaaaaa atatgatgat taaagctagt attgtgtagt agttaaacaa taagttcata
     2221 taagatatag aataaataat ttagtaaaaa agtatagcaa ataatttgct atgctttttt
     2281 tatgaaatca aaaaaataat aaaaatactt gactgtaaac cttgtttata cattatttta
     2341 tagaaaataa gatagtttta aatataaaaa aagatatatt attgaaagga gatattattg
     2401 tatgaaaact tataaaagat aattagtttg attttaagat atactaaata tacaatttta
     2461 tagagtgaaa atatttaaga ctatattata gttataaatt aatagaatag agaggtagtg
     2521 tttaaattgc aataacagta tttccaaagt tgccacctca aattcctatc caatggaata
     2581 atggtgtagc taattatgta gataaaaaat ttatttatgc ctaccctgtc atttgtgttg
     2641 tattcagaat ccttttacgt ccatttatat ggagatggtt acaaatacat gcttttatag
     2701 tgatactatt gcagattatt taacgaattt tatgtgtttt gtagcgttgt ctatagaaat
     2761 attctctata ttttttattt acaatattgc cagacatgtt acaataatta tttttataga
     2821 tatacttgta tttattgctt tactgataat aggtgtgtat aaattattta aaaaatacaa
     2881 ttaattttta aaaataagta ataagtgata gtgtaaaaag tagccactgc aaattatgga
     2941 ttgtagtggc tattttatta tttgtatatg aaattatttt gtttttctaa taagttacga
     3001 tatattatgg tttatttcac tgataaaagg tttaggtatt ttaaagtaaa aagttgtata
     3061 ttctaataaa atttaaacat tttgattctt taaaattaga gaaaatacat tgcttttatt
     3121 aaaaaattaa atattaagaa tttaatttct gcatcttatt tagaaaagat aagaaatcaa
     3181 ctttatatat tttttttggc cttcctcttt gattatcgag ttttgtattt acaatagttg
     3241 caattcctgc tttatataag atactcaata ttctattagc acttctaaga gttatgttca
     3301 tatagtctgc taatatttct gctgacacat tatttgaatt tctatcatta atcatacaca
     3361 tgattttaga tacatttgta ttagatagag gtataaaaga agcaacttta ttggctattg
     3421 agctatcttc aatattatta gaatttttat ttgaatataa aggacctatt gtatcattag
     3481 tatcatttac tataaaagta cagtttccat tcaaagaagc agatttacca ttagcttgac
     3541 gagcattttg ctctgcatga atatttgtat ttccaatgcc ccaacctata tttaagctat
     3601 atttaatttc ttttaaatct tctgaaataa aaaaatctgt atagttattg gtcatattgt
     3661 ttaacatttc tttagtagtg tgtatctcta ttttcatatc attttttttg acctgaactc
     3721 catagaatcc atgtcttgat agagaattta atatagtgtt atatatttga ttttgtatct
     3781 catcattttt aaatatcaaa tcttcattta tgtactctga actatttact gtaactatac
     3841 aagttgcaga tttatttttg tctaagtttt gtaatgttat ttcattcacc aaagatttaa
     3901 atgtattcag gattgattct gtagatggaa ccatgtaaat acaatcatat ccaagcttat
     3961 taagttcaca actaatgatg taatttctag taaaagaaag gttaatctta ttatctcgca
     4021 ataagtctaa atgtatttcc aataatttat catttaattt tttaaagtca tatacaggtt
     4081 gagggatatt aaaatcttta attgtatatg gacatttatt agagtctatt aatgaatata
     4141 tatcataata attgttgtcc tctcttaaaa aatccataaa tgattttgta aagtcaatat
     4201 ctttttggct atttaataat ttgaataagt atttataaaa atctctttca tcaagcttta
     4261 aaaaatttaa tggaactttt atgtctttta tatttattat aagttcacta tatggaacta
     4321 taccactgca tacaaatcca tcaaaattat ctacttgctc atagtatata ttaggtaaat
     4381 tattaaaagg attctcccct ttaagctcaa taaatgtaat ttcacaagta tcatttaatt
     4441 ctttaagagc ttgtttaaac ttatctgaca taaaataaga tgttgtcaaa aaaccaattt
     4501 tatatttcat aaaacctcct ttaaaatttc gagagtattg taactaatca atcaatttat
     4561 aacctcattt tataatagta gataaaacgc gtcaatttat tttacaaaag gaaaattgaa
     4621 agaaaaatca gaaaatataa aaaaagactg aaaaatatgt tggctgtcaa gaataattgt
     4681 gctattttgt aattaaggac tagtctttta agatttccta aaataaactt agattttagg
     4741 aaagaaatac attttatttg aaaataaagg agaagattat gaatacatat ttaaatgaaa
     4801 tttctaagtt tgttgatgag aatagagaag aaatagtttc attatggaaa gagattgtta
     4861 atatggaaag ctatacacat tgtaaagaaa gcgtaaataa attggctaag agattaaagt
     4921 ttgagtttga aaaagaaggt ttagattgcg agttagtaga tgttggagat aatggtagta
     4981 cattgatagg tacacttggt tccaatatag ataaaaagcc aataattttt tcaggtcaca
     5041 tggatactgt ctttgaaaca ggtacatttg gagagaatcc atttaaaata atagaaggaa
     5101 aagcatatgg cccaggtgtt ttagatatga agggtggtat aataatttcc ttatatgtta
     5161 ttaaagcttt aaataaaata ggatacaaag aaagaccaat aaaaatagta ttttctggag
     5221 atgaagagat aggacataag gattcaacag gtgcagatgt tatattgagg gaagcaaaag
     5281 gtggtctttg tgcttttaat atggaaactg gattagtaga taatagtttg tgtgtaggta
     5341 gaaaaggaag aataggatgt aatatacatg taaaaggtgt agaaacacat gcaggtaatg
     5401 attttgaagg tggaagaaat gctatagaag aaatggctaa taagatacta cgcatacaaa
     5461 aattgactaa tttagatgtt ggaactacag taagtgtaag tgtgattaag ggtggaagag
     5521 tggaaaattc tattccagaa gattgtagta taaaagtaga ccttagattt gaaaaagtag
     5581 aagaaatgga gaatgttaaa gaacaagtta gagagatatg taaagaaact tacatagaag
     5641 gaacttctac agaagttaat tttataagtg aaatgatgcc atttgaaact acagaagatg
     5701 ttatgagatt tcatacattt gtaaatgaag tgtcaaagga aaatggattt ggagaattaa
     5761 atgctaagag acttggaggt agttcagatg caagttattt aactattgca aacgttccta
     5821 caatctgttc atttggtgta agaggggaat ggaaccacac atcaagagaa tatgcagtag
     5881 taaattctat gtttgagagg gtgaaattaa tttctacagt tattttaaac ttagataaat
     5941 ttgaggtata aagggggatt ttatatggat gtaacagctg gagataataa ggtaacatgg
     6001 aaagggtatt tatctttatt tgtattgata gtattatttt caggagtgtt taaagatagt
     6061 caaggcttcc ttaaagcatt agattttgct aatctgacag gttcatttgg tactattgca
     6121 gatggtgtag actttatggg taaaggagga acgggagcaa aagatggttt cttatttgct
     6181 ctgactttga ttccaactgt ctgttttgct gtaggtctta tagatgtagt tgaatctatg
     6241 ggaggaatga aagcggcatc aaagatattt aatccattgc ttagacctct gcttggtata
     6301 cctggaatag caggaatagc atttgtatct tcatttacta gttcagatgt agcctcaata
     6361 atgacgaaag aattatttga aagtggggaa ataacagatg atgaaagaac tatatttgta
     6421 gcatatcaat atgctggttc agctgttata ctgaacacaa taaatactca agctccactt
     6481 ttaccaatag cattattggc attaggtcca ataatcctta tagagatatt ctgtaaaata
     6541 cttggtgcta atctagttag attaatcata ggtgtaagaa ataaaaagaa taaggctaag
     6601 gaggctgtgt agtatgagtc aacaaaatac tggaaatgca attgtaaata gtaaacctaa
     6661 gaaaactgct gttgagatat ttatgggggg agcaaaaaaa ggtttttaca taggtgttga
     6721 acagatatta ccagccatga taatgggtta tgtaatagtg cagtttttac aattaactgg
     6781 tttagttgat attttaggaa atatttttgg accagtaatg ggtatttttg gactacctgg
     6841 ggaatctgtt gtagttttga tagcagcatt ctttgctaag gcagcaggag cagctacagc
     6901 tgcaaactta tttgcacaag gtctaatcac tgcaccacaa gcaaccatat taattatgcc
     6961 atgtatgtta atgggtactc tagttggtca ttatgccaga atagtgcttg tagcaaatgc
     7021 cagtactaag tatagaatgt tactgttggc agttcctata tttgatgcaa tagtggggat
     7081 gttaatagta agagttttac tcactgttat gggattaatg taaataacat aaaattataa
     7141 cctaactata aatgatatat aagaattaat atatacaagg attaatgagt agtaattgat
     7201 atatagtaat aaactaagat agtaattaat gtgtatatta attaaaaaaa gagagtatct
     7261 caaagtgaac tttttagttc ataagatact ctttttgtat gaaatcaagt atattttcat
     7321 aatttcaaca atgaaagaga ggcttatctc tattttgaga cagcctcttc ttttaaattt
     7381 atatttctag aatttatgtt ttttatttat ccaacattaa cattagttat tagaaataat
     7441 tttaagaaaa atatactttc atgttaatat cagaataaat gtgtttttaa aggatgattt
     7501 gttttgaatt ttcacgaata tctacttcta tgactttttc tattgtatct cctctaaaac
     7561 ctaataaatt acttgtcaag tttgcagtgg aacaagaatg attaggtata attcttattc
     7621 tatcaccaat ttttatatca gtttcaccat ttactttaat ttttccaact tcttctgaaa
     7681 gtgaatcaat agtaagttct ggatgtccac ataccctacc ataaccaaca atactactat
     7741 ttccatgtgc acctttatca agtccaaggc acttacttcc acaatcaatt aaatagtatc
     7801 cactgctatt ttttgagatt acagtgcaca caacagtaag agcacacata tcttcagtag
     7861 cacaacctaa acctatctgt atagcatcat agtatacata gtttccaggt cttacagcag
     7921 ttatagtttt agaagcagct tcagattcaa ctgttggagt actaccagaa gcgacaattt
     7981 ctatattaaa tccattatca gttagagctt tgtatgcttt ctccatagaa gaatcttctt
     8041 gaacacaaac atctggaaca ccttctggag aactacaacc atacacttga cctggatgcg
     8101 aacttatacc aacaatcttg atgttttcta gcttagaaat ttcagtagca agttcaacca
     8161 ctttttctgg ttcaacccca aaacggtgta agccacaatc tacaataata agtagcttgc
     8221 actctatttt tgatttagaa aaagcttcac tataagcctt agcacattct atattatctg
     8281 tagcacagat taaatttatt tttgatgcca tagtcacaat tctatttata tttacttctc
     8341 ccatgtaagg ataagccaac ataatatctt caaaacctgc ttttatcaga gcttcacctt
     8401 catcaagtgt tccaactaga aaaccactag cacctgcatt tttttgaagt ctcgctatgt
     8461 aagtactttt atgtgtctta agcataggcc aaagttgttt attattattt gtacaaattt
     8521 gttgaatttt atctatattc ttttctaact ggtctaaatc tagcaaaaaa tttggagtag
     8581 cgacttctga aattttcatg ttatttacca cctttcaaca aaagtaaatt aactataagt
     8641 attataatta ttatattagt aatatcggct ttataatatt aatagcttat aattatatta
     8701 tagaactagc gttaacagag agcaatattt atttgtaata attagaattt tcaataagtt
     8761 agtgtagttt taaattattt atataatata tttttaattt tatgcaaagt ttatttaaat
     8821 tagtttgagg atttattaat agggtattat tgaatgagta aaaaagtgat gtttacatag
     8881 aataatcaca ctataaatta atttagaacc taaaaaatta attgagcaaa ttggaaaaga
     8941 gaaggataaa aaagatattt ggatagttgg tggaggcgat atagtaaaat tatttatgaa
     9001 agataactta attgatgatt attatatata tatattgcca ataatattgg ggagagggat
     9061 ttcactattt aagtctggta tagacaagac taatttgaat tttaagaaag ctagtaatat
     9121 tggagaactt gtaaaattgg aatacagtaa aaaacaaaaa taaattacat gccaatttgt
     9181 tacaatgtta tgaaacgaag catttaaaag acttttggct atatgaattg acattacttg
     9241 tcaaatatgt taacatataa gcgacttgtc ggagggtgta ttaaaaagaa tatgctggag
     9301 aagagagtgc gttaactctt ttctcctttt tttataaaat taagcattta taaatttagt
     9361 attattacat gattacacta tctcaaaaat taagaaagga gaaaaatatg aaattaattt
     9421 taagttattt aaagaattat aagttattaa tagtattaaa catactggct atatttagtt
     9481 ttgcattagt cgagttgggg attccaacta taatagctaa aataattgac aatggtattg
     9541 caaatcagaa tatagcttac ataaaacaaa tgggaatagt gattgttatt atttctatta
     9601 taggggttgt aggtagtata ttgttgggtt attgttctgc taaggtatca acaagtgtaa
     9661 ccagagatat aagaaatgac atatttgaga aatcacaaga attttcacat actgaataca
     9721 ataaatttgg aatttcttca atgattacaa gaacaacgaa tgatgcattt caaattcaac
     9781 aatttgttaa tatactacta aggactgcct tattaactcc agttatgttc attataagta
     9841 taataatgac aattagaacc agtgtggaac tttctatagt attggctata tcagtacctt
     9901 ttataattat tggagtagca ataatagcaa aagtatctca ggcaatatct tcaaaacaac
     9961 aaaaaggact agataaatta aaccttatat cgagagaaaa tttaactgga ataagagtaa
    10021 ttagagcttt tggaaatgat gattatgaaa cagagcgatt tgaaaaaaca aatacatatt
    10081 atgctaatgt ttctaagaaa ctatttaagc ttatgtcaat tactcaacca gcattctttt
    10141 tactgttaaa tatagctgtt ttggctgtat tttggatttc aagtgagaag ataaatattg
    10201 gagagcttca agttggacag ttggttgcat ttttggaata tcttttccat gccatgtttt
    10261 caattatgct tttttcaatg gtatttatta tgtatccaag agcagaggta tctgcaaata
    10321 gaataaaaga actattaaat gaagaaccat tgataaaaaa tcctgaaaat ggaattaagg
    10381 atactgaaaa taagggtatc atagaatttg ataatgtaac ttttacctat ccagatggag
    10441 aagcttctgt acttaaggat atttcattta ctgcaaaaac aggtgaaaca gtcgctttta
    10501 taggaagtac tggtagtggg aaaagtactc taattaactt gatacctaga ttttatgatg
    10561 taacagaagg tagcattaag ataaatggag tagatataag agaatatgat ttaaaagctt
    10621 taagaaaaaa gataggattc atacctcaaa aatcactgct ttttactggt agcatagcaa
    10681 ataatattag atttgggaaa cataaggcag gagagtcaga attagaatat tccgcaaaag
    10741 ttgcacaagc atatgagttt atatcaaaaa aacctagaaa gtttgatgaa ttgattagtg
    10801 aaggtggagc aaatgtatca ggaggtcaaa aacaaagact ttctattgca agggctatta
    10861 taagaagacc tgaaatatac atttttgatg acagtttttc tgcacttgac tttaaaactg
    10921 atgcaatact tcgtgctaaa ttgaagaagg aaacaaaaga tgcaattgta ttgattgttg
    10981 cccagagaat tagttctatt attgatgctg ataagattat agtactcaat gaaggacaag
    11041 ttgtaggaat gggaactcat aaagaattat taaaaaactg tgaaatatac tatgaaattg
    11101 caacatctca gttaaaaaag gaggaattag aataatgaaa aagtctaaag gttttaagaa
    11161 aacaattggt agacttcttc catttgtaaa gaaatataaa ttttccttta ttatagctat
    11221 tatatgtata atttcagcag ctactatgaa tgcacttgca cctaaaactg aaggattgat
    11281 aataacacaa ttgactaaag atgtaattag tatcgcaaaa ggagtaccag gggcttccgt
    11341 aaattttgat tatgtaacaa aaatattggt gattttagca tgtatatatt ttgccaatgc
    11401 aatttttacg tatacttcaa gttttctact tacgaatgct attcaaaaca caatgagaga
    11461 tttaagaaat gaagtcgaaa ataaaataag aagattgcct attagttatt ttgatagtaa
    11521 tagttttgga gatgtattaa gtcgtatatc aaatgatgta gatacaatat caaatgcatt
    11581 gcaacagagt tttatgcaaa ttgtaaactc aatcttagtc ataattttgg ctctttcaat
    11641 gatgtttaca attaatatat atatggctct tatagctctt tttataattc caattagtta
    11701 ttttgtatct aaatttgtag taaaaaaatc tcaaagtaga ttttcattgc aacaaaatgc
    11761 tcttggaaaa ttaaatggaa aagtacaaga aatgtatact ggatttaatg aaataaaact
    11821 atatggtaaa gaagaagatt ctataaaaga atttaaaaaa gtaaatcaag aactttgtga
    11881 aaatggattt aaggcacaat ttatatcaag tatgatgaat cctatggttt cattagtaac
    11941 ttactttgga attgcagctg tggcagttgt cggttctata tatgcagtaa gtggaggaat
    12001 aacagtagga aatttacaag catttgtgcg ttatatatgg cagataaatc agccactttc
    12061 acaaatgact cagttatcta cagtaataca atcttctttt gcagctatag aacgtgtatt
    12121 tgaaatttta gacgaagaag aggaaatacc tgatgttgaa aatccagtta agatagaaaa
    12181 tgtaaaagga aatgttacat ttgagcatgt taattttgga tatggagaaa attcaacttt
    12241 aatagaagat ttaaatgcag aagtaaaaag tggtcagatg gtagctattg ttggacctac
    12301 tggagcaggt aaaacaacat taataaactt attgatgagg ttttatgatg taaagaaagg
    12361 tgctattaaa attgatggag tagatataag agatatgaaa cgtaaagatt tgcgttcaat
    12421 gtttggtatg gtgcttcaag atacatggtt atttaatgga accatttttg aaaatataga
    12481 atatggtaga tttggggcta ctaaagaaga aattatacaa gcagcaaaag tagcaaatgt
    12541 tcatcacttt ataaccactc ttccagatgg atacaatatg tttttaaatg aagaagcatc
    12601 taatatatct ctaggggaaa aacagctttt aaccatagct agagctttta tatcagaccc
    12661 ttcaatattg atacttgatg aagctacgag ttctgttgat actaggctgg aacttatgct
    12721 tcaaaaagca atgagaaatc ttatgaatgg tagaactagt tttgttatag cacatagact
    12781 ttctacaatt cgcaatgctg acttgatttt agttatgaat aatggaagta ttatagagca
    12841 ggggaatcat gatgaattaa tggaaaaggg tggattttat gagaagcttt ataatagcca
    12901 atttgcagac aaagaatcag aatagataac ataaactttt gtataaaatt ttgtatcaaa
    12961 ttcaaatatt aatcatataa ttaaaaagag tatcagtaag ttgttttttt atagatttac
    13021 tgatactctt tgattttaag actaaaggta tttatgattg gtacttataa ttcacaacta
    13081 gtcttgttac atctgataac cagaattaca gtacttttct tttttacctt tttgttctct
    13141 aacatataac aattcgctta acttatcaat gtaataacca tcatcactta tgcgaattaa
    13201 actagtcaga taatatccat ctttgcttct aggaatcttt ttatttattg ctaaatcaat
    13261 agctgtcttt gctgcttcaa aatgctttaa atgtgtatga ccttcttcaa aactctttct
    13321 agtattataa actacataac cttttttaac ttgaaaaact ataaattctt tcttttcata
    13381 tattttatta aacattcaat cagcccttct caagatagtt ttatagcata acattagaag
    13441 cttctaaaaa attttacaaa gaacataaat attttataaa ttataaatct aaaactataa
    13501 tttataaaga taactttctg attatttata tgataattca tatagattaa aagtaaatta
    13561 tc
//
LOCUS       DAEOSP010000106        13560 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.106, whole genome shotgun sequence.
ACCESSION   DAEOSP010000106 DAEOSP010000000
VERSION     DAEOSP010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13560
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.106"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            88..420
                     /locus_tag="KRH54_002309"
     CDS             88..420
                     /locus_tag="KRH54_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBG8579966.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            582..767
                     /locus_tag="KRH54_002310"
     CDS             582..767
                     /locus_tag="KRH54_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579967.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            794..1879
                     /locus_tag="KRH54_002311"
     CDS             794..1879
                     /locus_tag="KRH54_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG8579968.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            1872..2330
                     /locus_tag="KRH54_002312"
     CDS             1872..2330
                     /locus_tag="KRH54_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579969.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            2365..3456
                     /locus_tag="KRH54_002313"
     CDS             2365..3456
                     /locus_tag="KRH54_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG8579970.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            3461..3685
                     /locus_tag="KRH54_002314"
     CDS             3461..3685
                     /locus_tag="KRH54_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579971.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            3695..4258
                     /locus_tag="KRH54_002315"
     CDS             3695..4258
                     /locus_tag="KRH54_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579972.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            4219..4848
                     /locus_tag="KRH54_002316"
     CDS             4219..4848
                     /locus_tag="KRH54_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8579973.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            5025..5777
                     /locus_tag="KRH54_002317"
     CDS             5025..5777
                     /locus_tag="KRH54_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8579974.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            5790..6275
                     /locus_tag="KRH54_002318"
     CDS             5790..6275
                     /locus_tag="KRH54_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579975.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYISKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            6282..6431
                     /locus_tag="KRH54_002319"
     CDS             6282..6431
                     /locus_tag="KRH54_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8579976.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSNDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(6568..7389)
                     /gene="yidA"
                     /locus_tag="KRH54_002320"
     CDS             complement(6568..7389)
                     /gene="yidA"
                     /locus_tag="KRH54_002320"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBG8579977.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            7882..9576
                     /locus_tag="KRH54_002321"
     CDS             7882..9576
                     /locus_tag="KRH54_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBG8579978.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYVRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIKNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      9908..10018
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10122..10493
                     /locus_tag="KRH54_002322"
     CDS             10122..10493
                     /locus_tag="KRH54_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBG8579979.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            10590..11537
                     /gene="trxB"
                     /locus_tag="KRH54_002323"
     CDS             10590..11537
                     /gene="trxB"
                     /locus_tag="KRH54_002323"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG8579980.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            11641..11958
                     /locus_tag="KRH54_002324"
     CDS             11641..11958
                     /locus_tag="KRH54_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBG8579981.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            12227..13513
                     /locus_tag="KRH54_002325"
     CDS             12227..13513
                     /locus_tag="KRH54_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBG8579982.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
BASE COUNT         5361 a         1426 c         2452 g         4321 t
ORIGIN      
        1 ctattttaca gataaataag aataaactta taatataatt gtatctgtaa aattttgtaa
       61 aatataaaaa gtataggggg aataaaaatg aaagtagctt atatttttag tagtactaat
      121 acacataaaa ttctagacaa aatgataata ccacaacttg aacaaggaac tcatggagta
      181 gacgttctag ggatgttttt ctttatggat aataccttat ttttatctaa aggtaatcct
      241 gtaggcgaga gactaagtaa attacatgaa aaaactgata tgataataat ggcttgtgac
      301 caatgtgcta tagaaagagg tatagagaat aatttggttg atggagctac aattggatgt
      361 ttcccaaacc tatatgcttg tttaggcagt gcaggtgtgg accaagttat aacgttataa
      421 attagtatag gatacaatcc atagaaaaat ttattttctg tggattattt taagtaaaag
      481 ataaattgtt taaagtaggg gatgaacaaa tgtctaaaat tttagttgaa tttataaaca
      541 cttgaccatc ttgtgatgaa catggagtgt ttataaaaaa aatgtcagaa aagtatgctg
      601 ataaattgga aataaaatta tatcaagcag ggaaagattt ttcgtatata aaaaaatatg
      661 ggattataac aaaaggaact ttaataataa atcagaaaaa gaaatatgat agattaaata
      721 aagatacaat tgaaagggca atagtagaag ctataaacaa taattaaaag tttaaaaatt
      781 aggagtatca aaaatgaata tattagaaat tgttttaaat ggagttaaag atactatagt
      841 agcttctata gttacactga atggagcatc aggatggctt atgataagtt ttatcttggc
      901 aggtgtactt cacaatgttt taactccaag aaggtttcaa aaacaacttg gaaataagaa
      961 attttcttct ttaattaaaa gtactatctc aggaatgttt ttaccaatat gtagttgtgg
     1021 agtaatacca cttggaataa gtatgtatta ttcaggagca tatttaggac cagtacttgc
     1081 ttttatgaca tcaactccaa taataaatcc aatagctgta gttctttgtc taggtctttt
     1141 aggtcctaaa gttacaatca tttatctaat agctggtttt ttagtaccat ttttagtagg
     1201 atttttagga aattgttttg gaaaagaaga attatcactt cataatgatt cagaggaaga
     1261 agtaatagaa ttagaagaag aaaaaagaag ttttatggaa aatatgattg atggtcttaa
     1321 atggtctttt ggtgaattag cccttactat tagtaaatat gtagtaatgg gtatggtttt
     1381 tgcaggattt ataacaacta tttttccaaa tagtataatt caaaaatatt taggaaatcc
     1441 aggtttgcta tctcttggaa gtatagcaat acttgcatgt gttatgtatg tatgtgctgt
     1501 aggacatata ccgtttatag cagcccttgt tgcaagtgga gcatctcctg gtatagcgat
     1561 aacatttcta atagctggag cagctacaaa tctaccagaa cttataagta tgtttaaact
     1621 tattggaaaa agaactgtta ttatatattc tgtaactttg acactgtctt caataataat
     1681 tggatatata acaaatttac ttttagtgaa taataactca gtaaatttag atgcaacaaa
     1741 ttcttcaata cagttggcaa ataaactaat gttaaatata cctgatagtt ttgagtatgt
     1801 atgtactatt ttaattattt tatttgcatt taaatcaata ataccaaaga tgaaagaggt
     1861 cataggaaat catgcttaaa aagatagtta taatatcctg catagtagtt gtgttaatca
     1921 ttttatcaaa aatagttgat gataacatca aagaagatgc ttctattcca aatgtgaata
     1981 aagaaacatt agaatatttt agaaaaaatt ataaagaaga cataataact tgtgctgaag
     2041 aagacttaaa taatgatggg aaaaaagatt tagtagttat atataaaaaa agcaataatt
     2101 caaatgaaat ggtagtcgta gtttctgata aaaatagcca ctatataaca aagccaatac
     2161 cagcacctat agagaatcaa actataacct ttaaaaatat tgatgataaa gcaccaatag
     2221 aagtaattgt atcagggtct aaaaatggta atgtaggata tgctatttac agagtagagg
     2281 gtaaaaaatt tgttgattta tttggcgaag atatggacaa atgttgctaa aaataaaaat
     2341 aatacataaa ggtgggaaat ttatatgaaa ttatctaaga agattcaagc attaatctta
     2401 tttggtgtaa tgggtacatc tatattaaca ggatgtagta gtaaaccaaa agaaaaagaa
     2461 gaagcaaaaa ctggaaaaga tgattataca ataacagtag gttattataa ttgtgaccat
     2521 atgactgcgg gaccagtagc agaatcggca ggaatatata aagatttagg tcttaatgta
     2581 aaaactgttg gaaatggtaa agttcctgaa gctatggcag caggaaaaat ggatgcaggt
     2641 tacataggaa ctaaggggtt agttggagca atacctaagg gttcaccaat aacaatagca
     2701 gctaacaacc atactggtgg ttctgaatat ttaatagttt caaaagatat aaaagaacct
     2761 aaagatttaa taggtaaaaa aattgcaaca gatatgagtg atttcttatg gacaagtgat
     2821 tatggtccag aaacaggtct accaactgac ccatcaaaat atgaagttgt aaatatggat
     2881 tcagacaaag ataaatattt agctcttaaa actgggaaaa ttcaagcttt tacatcttgt
     2941 gacccttggg gttctgtagc tgaaaatgac ggtgcaggaa aaataatagc atctactcaa
     3001 tataaagaaa aagctaatgg caaagaatat aactgttgtt cattctcatt aaacaaaaac
     3061 tttataaaag aacatccaga tttagcaaaa aaattggttc tagcacatac aaaagcaata
     3121 gaatatatat atacaaatcc agctgaagca gctaaaatat ttgcaaaata ttataatgta
     3181 gaagaagaag ttgcacttag aactatctat aagaagactg ttggagaagg tagaactctt
     3241 acttggaaag ttacaggtga agaatataaa aataatttac aaatgtataa agatttaaag
     3301 gctttagatg atatacctaa atatgaagat acgattgata caagcttatt ggactcttgt
     3361 ggagctgatg attttgacaa atttataaaa gaaaaagtta acccagagtt tcctgaaggt
     3421 atgagttatg aagactggaa agcaaaggta ttataaataa atggagaata tgaaaaatat
     3481 aataatactt tttatcatta ttttgggaag tatatttata atatttttta attttgaaga
     3541 taaagaagaa ataaaaggaa aattaagaat aggggtatct gatgatacct ctggttttgt
     3601 aataaattat atggtaaata gagactactt tgaaaatata aaagtaagtg atataatgga
     3661 tatttttaca attaatgatt gctgagcaag tacaatgcag tgggctctga gttcagagga
     3721 aatagatata gcaatcatat gtaaagatgc tgcaaaacaa tatgtaaatg taaatagtgg
     3781 atttgaaatt gttggtactg tagttcaaaa ttctgatata tttctattaa gtgataataa
     3841 tcctgaaaag ataggagtca ttcaaaatag agattaccag tcagaattgg ttaaaaaata
     3901 ctacaagaat gtggaagtag ctcccttatt agggactgca ctagcatatg gattagaaag
     3961 taatttagta gatggtgttg taatagattg cataaagtca attgggttag agggcaaaag
     4021 aaaatctaca actaaattag gagattatga tacctatgta ttagttgtaa gtaaagagtt
     4081 taaaaaaagt aaagcatata gtgattttat aacactttac aataaaagtg ttgatgcttt
     4141 aaagaataaa gctacattta agaaagcatt aaaagagtac aggaatataa gaaatataga
     4201 tatggaggaa ataagcaatt ggaaattaaa atttctaaag ataaaagcga tagattaact
     4261 aagaaaaaaa ctgatggtag tatggatatt ttatataaaa taatactatt agttattata
     4321 tttttagtct ggcattttgc agcaaaagat atagggagtt ctttgttact tccaatgcca
     4381 gtagatgtaa taaaaggttt ctttttctgt gtgacagatg cagagacagt cactaatctt
     4441 tttataacat tgcagagagt acttaaaggt tttatgtatg cattattgtt tgggcttcca
     4501 ataggattta ttatgggatt ttctaaaact tttgaaaggg ttttatctcc agtagtagat
     4561 tctgtaaggc aagttccaat aatggcatgg gttcctctta ctatagtatg gtttggtata
     4621 ggagatggtc caacaatatt tttaatagct ttttcagggg ttttccctat aattttaaat
     4681 actattcaag gagtaagagc aatatcaaag gattattata atgcagctag aagtatggga
     4741 gcatcgccaa ttgtgatatt tacaaatgta attgttcccg catctcttcc agatatacta
     4801 actggttcaa ggattgctat aagtacagga tggatgtctg ttatctgagc cgagtttata
     4861 gctacgagtg ccggtctcgg ttactcaatg gtacaagctc aaacattaat gcatacagat
     4921 gtattaattg ggcttatgat atttgcagct ttaataggat ttataataga tagggtatta
     4981 aagtttataa ataaaatcct ttgtagatgg aggtttgcag attaatgaag ttaagtgtaa
     5041 gagatataaa taagacattt gtaaataata gagtacatac aaaggtttta gaagatataa
     5101 gtatggatat agatgatgga caatttgtct gtcttttagg accatcaggt tgtggaaaaa
     5161 caactttact tactataata ggtgggtttc aaaaatcaga acgtggagat gtttttataa
     5221 atgataaaag agttaaaaaa ccgggcatag atagagcttt tatatttcaa aattatgctt
     5281 tatttccttg gaaaactata agaggtaatg ttctatttcc tatgaaacag cagaaaatac
     5341 caaaagaaaa aagagaagaa atgctagaag aacttttggt tatgtcagac ttgaaaggaa
     5401 aagaaaaact gtttccgcat caattatctg gaggaatgaa acaaagagtg gccatgataa
     5461 gagctttagc atgtaatcca gaggtattac taatggatga accattagga gctgtagatt
     5521 ttcaaatgag acaaaattta caggaagagc tagaacgaat atggataaag aagaaaataa
     5581 ctgcattaat ggtaactcat gatgttgatg aagctgtata tatgagtgat agagttatag
     5641 taatgtcgag agataaagga agaattattg atgatataaa tatagatatt ccaagaccta
     5701 gaattagagg aagccaaaaa tatgaagagt acaaaaacaa attaactgat actttatcaa
     5761 agtgttatga agtttaaaga ggagtagaca tgagtcaaat agaaaaatac aatagtttgg
     5821 ataatacaca acagaaaata agaatctcta ttatggatat gataatagat aaaggttcta
     5881 gtgttacact tcaagaagtg actgaatata taagtaaaaa gttgaatata gaaaaagaat
     5941 atatagaaag gacactacaa tattttattt ataaaaatat aatggttgtt gatgggaata
     6001 gtataaattt tatatatcct gtatcagcat tgccaacaaa tcataaagta actttgagag
     6061 ataatagaag tttcagtgca atgtgtgcta tagatgctat aggaacctct tgcacattca
     6121 accaagatat aaaaataaac tctatttgta gtgctacagg taaggaaata gaagtaatta
     6181 taaaaaatga aaagattgag tatgtgaata atccaaactt aagagtgctt catataaatc
     6241 ttgataagca tctaaattgg gctgctagtt gctgaaatat aatgaatttc ttctggacga
     6301 agagtgattt tgatgcttgg acaaacgaag cgggattatc taatgatgaa gatatatatt
     6361 gtttagatat taatgaggca atagtagaat catataaaat atttaaactc aaacaaaagg
     6421 tactatcata gatatacata tatgaaaaac taaaaatgac ctttagataa aaatgatagg
     6481 taatatccat actatataaa ttagtataga gtagggatgt gaaaagtatt taaatctaaa
     6541 ggtatatttt tagttttttt tagatgatta gatagcctta ttaagtatat atttatttat
     6601 aacctttgca actccatttt cattattagt atcagtaaca taatctgcaa tttcctttat
     6661 actattacgt gcattcccca tagccacacc aagacctgca tactttatca tatgttggtc
     6721 attttcttca tcgccaacag caatagcatt ttctataggt atatttagat tcgtacataa
     6781 tgttttaaga gcagttcctt tattagtagt tttacctaaa aattctagat aaaaaggagc
     6841 agaccttact atagtatatt tattgaataa atttttaggt agttgctgta tgatttcttc
     6901 caatctttct ggttcatcaa ctatcataat tttacagatt ttaatagatt catcaatttt
     6961 atctttttgt atatatttga cttctatatt atttaatttc gcttcatgaa gtgtatattt
     7021 gccaggcgtt tcatttggtg taattaaatt tgtcattgta tttatgtgta ttttagtctt
     7081 atatttttta caaacgttat tatatataaa atcaaaatca tctaaactca tttctacatc
     7141 atgtatgatt ttctcacttt taacttcttg aataattgct ccattaaagc atataacata
     7201 atcaccttca tctgttaaat ttagttgatt tagaagttct tttacaccag gaagaggtct
     7261 gcctgttgtt ataactactt ttgccccagc cctttttgcc tcctgaatgg actcaaagac
     7321 ttcaggagta atcctttttt gtgtatttaa tattgttcca tcaatgtcta gtgcgattaa
     7381 tttgtacata tcaacctcca ataaattata tactcaaatt tcctgtgaaa acgttgacac
     7441 agctagtgtt gaacaaagtt tcagtgggaa atatggtttg ttttgtatac tattatatca
     7501 agagataagt aatttagaaa gtttaataat attaattttt tgataaaaat attacttatg
     7561 tgagatgaga atgtaaaaac tttgtaatca ataattccaa aattcttaat tacaaataat
     7621 ggtatcgttt gcattgttaa tataaataaa tgtatattat atagttagaa gtttgaataa
     7681 atagactgat ggtaatttgt tatattagtt agttttgtgt tataaaatat tgtaattcca
     7741 ttaatcatat gttattattc aacatgatta taatataaag aatatttgat atatataata
     7801 atttataaaa gagtattaca tagtacatgt aaatctatat ttataaatac aataaagtaa
     7861 agttgtaaag gagtgcttta aatgaagttt tcagagttta agtatgaaag acccaattat
     7921 aatagtatga aaaaagagtt tttgagttgt gtagaagata taaataattc tcgtaattat
     7981 aaagagcaac aaaaaaatat acataagatt aatttattaa gaaataaaat agaaacgttg
     8041 tcaaatatag cttcgataag atatagtaca gacacattta ataaattcta caaagaagaa
     8101 aaaaattatt gggatgaata tatgcctttg tatgaagaat taaattcata tttttataat
     8161 gccattgtaa attcaaaatt taaatatgat ttgataaaag agtttggaga gcaatttttc
     8221 acaatagtag agtattcttt aaaaagtttt tccaaagaaa taataagtga attgcaggaa
     8281 gaaaataaat tgtgttcaga gtatactaga cttttagctt cagctgagat aatgtttgat
     8341 gggaaaataa gaaatttatc tggtatgggg aaattcatgt attctaagaa tagaaaaaca
     8401 agagaattag caaacaaagc atattataat ttctttgaag aaaatgaaac taaatttgat
     8461 gacatatttg ataaattggt taaacttaga gataagatgt ctaaaaaatt aggttttgaa
     8521 gattttgtag agcttgggta tgttagaatg atgagaagtg actacagaga atatatgata
     8581 aaaaatgtta gaaaacaagt actaaaatat gttgttccaa tggcaaatga gctttatgaa
     8641 aaacaagcta aaagaattgg tctagaatat ttaagttaca tagatgaagg cgtggaattt
     8701 ttaacaggaa atgcaagttt aaagggagat tcaagataca taattaaaaa tggtaaaagg
     8761 atgtattctg aactgtcaaa agaaacaaat gaattttttg attttatgtt agaaaatgaa
     8821 cttatggatt tagaaacgaa gaaaggaaaa ggagctggag ggtattgtac ttatatacca
     8881 gattataagt caccattcat tttttctaat tttaatcaaa ctgctgatga tatagatgta
     8941 ttaacacatg aggcaggaca tgcatttcag ttgtatatgt ctagatggat tgatatgcca
     9001 gaaattaatt ttccaacgct tgatagttgt gaaatccatt caatgagtat ggaatttata
     9061 acatggccgt ggatggattt attttttaaa gaagatacag ataagtacaa attcactcac
     9121 ttatcatctt caattaaatt tataccttat ggagtaattg ttgatgaatt tcaacactat
     9181 atttacaaaa atcctaatgt agataagtca aaacgaaaag aaatctggag atttttagag
     9241 aaaaaatact tacctcatag aaaatatggt gataattctt ttttagagag aggttgttgg
     9301 tggtttaaac aaggacatat atttaaaaat ccgttttatt atatagatta tgtattagct
     9361 caaatttgtg cgttacaatt ttggaaaaaa atgattcaag atagagatgc aggatggaaa
     9421 gattatataa atatctgtaa agttggagga acaaaatcat ttttagatat tgtgagtatg
     9481 ggaaatttgt attctccatt tgatgatggc tgtattgagt ctattatagg tgatgttaaa
     9541 tcttggtttg atgaaataaa tgacagcaaa ttataaaaaa ttatgagaat tttattgtat
     9601 aaaagtgaaa aattaaaaaa tatataaaaa gtgattattt gttgtgaaaa tagtcttgtt
     9661 tttaactaaa ttgataaaat aaactaaaaa agatttgtac ttaaaaaaaa aagaaaatac
     9721 aagaaatatg caatatcaac atttggaaaa aaaagtaaaa atgtatacaa aatacatata
     9781 aaaaaactat tgcaaatttt atagaatgtt tatatactta agtcaacaga gatagaaaaa
     9841 agctatttat agaaaacaaa ggataaattg cttaatttta aattttctga aaaaaataat
     9901 aatccggatg aagagtatgg gagactgctt tataaaaagc gaccgaaggg acaagtttaa
     9961 tcttacttac ccaaaagatt agatgaaatt ctcaggtaaa ggtgactgta cttggacggg
    10021 gctctgaaaa tgattttaac agggaagaca aattgggaga tttgtttttc ctgtttttta
    10081 tttatcaagt aataaataaa agtttgaaag agagtaaaaa aatgataata attacgaata
    10141 atccaaaagt aaaagaagaa gttcaaggca gagaagtttt gtttaaagac acaacttata
    10201 ttggaatctt agaagcaagt agagatttga tacatgaagg ttatgaactt ttatctcatc
    10261 cactctatgg gagtgtaaag ccaaatgaaa caccttacag gactgttata ctaaagaaag
    10321 gaaatcgttt ggatataaac tctttaacct taattgaaga agcaatcatc actgcaagta
    10381 aatttcagaa taataaaaag actccaaaat ggacagaaag tgttcaagat gattttagag
    10441 taattgatta tgatatattt tataatacaa tacaaagaat gcagtatgaa taaaaaaaaa
    10501 ttgttaaaaa gttattaaaa cagcctaaat attaaatcta atttaatatt aaataaaatg
    10561 agaaatttaa ttggggagga taagtcaaaa tggaaaatgt gtatgactta gtaataattg
    10621 gttcaggacc agcagggctt gcagcaggtc tttatggagc aagagcaaag ttaaaaactc
    10681 taatcctaga aaaagacaag actggtggac aaattgtaat aacacatgaa attgcaaatt
    10741 atccagggtc agttccaaat gcaacaggac caagtttaat agctagaatg gttgaacagt
    10801 gtaaagagtt tggagcagaa atgctaagag acaacattgt tgatactgag ctagatggag
    10861 atataaaagt tttaaaaggg gaaaaggcag aatatagagc aaaagcagta ataataggaa
    10921 caggagcaac accaagaaag attggttgtc ctggagaaaa agaacttact ggtaaagggg
    10981 tttcatattg tgcaacttgt gatgcagatt tctttgaaga ttttgaagta tttgttgtag
    11041 gtggaggaga cagtgcatta gaagaagcaa tgtatttaac taaatttgct agaaaagtaa
    11101 caattgtaca tagaagacaa ggatttagat gtgctaagag tgttgaagaa aaagcaaaag
    11161 ctaatccaaa gatagagttt ttattagata cagtaattga agaaatcaaa ggtgatggaa
    11221 tattagaatc agttgtattt aaaaataaag ttactggaga aactcatgaa tactttgcag
    11281 atgaagaaga tggaacaatg ggagtatttg tgttcgttgg attagatgct caaactgatt
    11341 tattcaaagg taaagttgat atggatgaaa aaggttacat aataactgat gaagatatga
    11401 gaactaacat accaggcgta tttgcagcag gagattgtag atctaagact ctaagacagg
    11461 ttgtaactgc aactaatgat ggagcaattg catctatagt agctgagaaa tacattgatg
    11521 aaaaatttgg gaactagaat ctgtaaatta agaaatagtg tctgtaaatt taatgaataa
    11581 tatttaaaat taaaattaat atttaaaata aaaataaaaa tttcaagggg gaataagaaa
    11641 atgttagatt tagataaggc tacatttgaa gaagaagttt taaatgcaga aggttttgta
    11701 tttgttgatt tttggagtga aggttgtgag ccttgtaaag ctttaatgcc agatgttcat
    11761 aaattagctg agacttatgg ggataaaata aaattctgta agatggatac aacaaaagct
    11821 agaagattag ctataaaaca aaaagtatta ggtcttccaa ctatggctat atacaaagat
    11881 ggagaaaaag tagacgaagt tactaaggat gatgctacag ttcctaatat agaaaacatg
    11941 attaaaaaat atctttaata tgttgataaa ttattaacat taaaagaaaa aatctatttt
    12001 tatctgaaga taagaagaaa aaggttttta tgtccaactt tgttaaaaaa ataattttaa
    12061 ataaaataat ttttttacaa taaatatttt ttagtaataa aataaagatt taaggcataa
    12121 aaacacaata atatatcttt aaaaaaataa gataaattgt aaaatgatat aattttgaga
    12181 aaaactcaaa tgcatataca caaattaaat aacaggaggt gcaagtatgc gtcttgaatt
    12241 agggaaaatc tttataaaag atattcaatt tggtgatgta acagaagtaa aagatggagt
    12301 attattcatc aataagcaag agatgctaca agaaattggt ggagatgagc atattaaatc
    12361 tatagatata gaattagctc gtccaggtga aagcgtaaga ataacacctg taaaagatgt
    12421 tattgagcca agagtaaaag ttgaaggtaa tgggggaatc ttccctggta tcatgtctaa
    12481 agttgacact gtaggagaag gaaaaactca tgctttaaaa ggtgtagctg ttgtaactac
    12541 tggaaaaata gtaggtttcc aagaaggtat aatagacatg actggagaag gagctaagta
    12601 cactccattt tctaaattaa ataatgtagt agtaatagct gaacctattg atggtttaaa
    12661 acaatacgct catgaaaaag ctgttagaat gataggattc aaagcagcaa tgtacttagg
    12721 agaagtagca agaaatctta ctccagatga agtttcagta tatgaaacta aacctcttct
    12781 tgaatcaata aaagaatatc cagagcttcc aaaagtagga tatgtttaca tgcttcagac
    12841 tcaaggttta ttacatgata cttatgtata tggagtagat gcaaaacaaa tagtaccaac
    12901 tttattatat ccaacagaat taatggacgg agcaattgta agtggtaact gtgtttctgc
    12961 atgtgacaaa aacccaagtt atgttcatat aaacaatggg gttgttgaag acttatatgc
    13021 aagacatggt aaagatataa actttgtagg tgtaattata actaatgaaa atgtttatct
    13081 agcagataaa gaaagatctt caaactggac ttctaaatta tgtaaatatt taggattaga
    13141 tgcagttata gtttcacaag aaggttttgg aaatcctgat actgacctta taatgaactg
    13201 taagaagata gaaatgcaag gtgttaaaac agttatagtt acagatgaat atgctggacg
    13261 tgatggaggt tctcaatcac ttgcagatgc agatgtaaga gctgatgctg ttgtaacagg
    13321 tggaaatgcc aaccaagttg tagttcttcc taagttagat aaagttattg gacatttaga
    13381 agtagtagat gtaatagctg gtggttctga tggaagttta agagctgatg gaactattga
    13441 agttgaaatc caagctataa caggtgcaac taatgaaaca ggatttggtc atttaactgc
    13501 taaaggatat taagaagtat tttattaaac gattgagtaa tgttaataaa acatattata
//
LOCUS       DAEOSP010000107        13437 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.107, whole genome shotgun sequence.
ACCESSION   DAEOSP010000107 DAEOSP010000000
VERSION     DAEOSP010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13437
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.107"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            7..543
                     /locus_tag="KRH54_002326"
     CDS             7..543
                     /locus_tag="KRH54_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBG8579983.1"
                     /translation="MDENKTFFISEKNFIYGNIKPDAFSKYKLKKHYLDESFNMIVSK
                     INYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSERWEFKHSLNKHVTYER
                     ELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMNDLKFSAYTCNSVINYI
                     LDSILSNTIKSYHIANCG"
     gene            complement(647..1426)
                     /locus_tag="KRH54_002327"
     CDS             complement(647..1426)
                     /locus_tag="KRH54_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG8579984.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(1484..2740)
                     /locus_tag="KRH54_002328"
     CDS             complement(1484..2740)
                     /locus_tag="KRH54_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBG8579985.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            2998..3897
                     /locus_tag="KRH54_002329"
     CDS             2998..3897
                     /locus_tag="KRH54_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBG8579986.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            3921..5369
                     /gene="ddlR"
                     /locus_tag="KRH54_002330"
     CDS             3921..5369
                     /gene="ddlR"
                     /locus_tag="KRH54_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBG8579987.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDIEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            5428..6234
                     /locus_tag="KRH54_002331"
     CDS             5428..6234
                     /locus_tag="KRH54_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG8579988.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            6260..8179
                     /locus_tag="KRH54_002332"
     CDS             6260..8179
                     /locus_tag="KRH54_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG8579989.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTPKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(8421..8768)
                     /locus_tag="KRH54_002333"
     CDS             complement(8421..8768)
                     /locus_tag="KRH54_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBG8579990.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENRVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(8755..10683)
                     /locus_tag="KRH54_002334"
     CDS             complement(8755..10683)
                     /locus_tag="KRH54_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBG8579991.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFLEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            complement(11095..13284)
                     /locus_tag="KRH54_002335"
     CDS             complement(11095..13284)
                     /locus_tag="KRH54_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBG8579992.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKFGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDIYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
BASE COUNT         4879 a         2075 c         1735 g         4748 t
ORIGIN      
        1 gataatatag atgaaaataa aacattcttt ataagcgaaa aaaattttat atatggcaat
       61 ataaagcctg atgctttttc taaatataaa ttaaaaaaac attatttaga tgagtcattt
      121 aatatgattg tttctaaaat aaactatcta tgcagtctta atatagatag tctttctaaa
      181 tacttttcag taagtagact tagtcaggaa ctaggagtca tatgtcattt tttatgtgat
      241 tttttctgtg tagctcacag tgaacgttgg gagtttaagc atagcttaaa taaacatgtt
      301 acttatgaaa gagaacttgg agcatttgca aaagatgttg atttatctaa aattaaatgt
      361 ggtactataa atagtagttt tgagaagttt tttactaaac tttatagcga ttacaaaaag
      421 aaaactgatt atatgaatga tttaaaattt tcagcttata cttgtaatag tgtcataaat
      481 tatatacttg actctatttt atctaataca ataaaatcat atcatatagc aaattgcggt
      541 taacttaaga atttttaaac ttatataaac ttataattga atataattaa aatgactgct
      601 ctatagaata taattttata cttctctagg acagtcattt tttatactaa ctatttatct
      661 ccattataac atctatggca ctttttacac tatctactac ataatttgca ctttgcttta
      721 catcttccct tgccttagac attgcaaaac tatatttaaa tccttctaac atgcaaatat
      781 catttggaga atccccaata gtagcaactt catctggact tacatttaac gtttttatta
      841 catgctctat acttcctctt tttgacacac ttttaggaac tatatttaaa gtatatgttc
      901 cagaaaaata tatttctaag acttcactaa actcactttt tagataaagt tctattctgg
      961 ataactcttc caaattttca gaacatacgc ctatatttcc tataagatta tcaaaactac
     1021 ttcttttttc aaagtctggg tcaactataa agtcactaga ataatgtcta aaagcagtgg
     1081 tatcactacc cacaattaca tcacccttat atttaaaata aagttgtgca tcacttgact
     1141 caaatctatc aatgactttt tttactaaat tatcatctat cgttctttta aaaattacat
     1201 tataatcttt atcatatata aatgaaccat tttcacatac atagtatcca ttcatatcta
     1261 aactatcttt gataatatta tgtgcttgtt taaaaattct acctgtagca acattaaact
     1321 gtactccctt tttttctatt tcttcaattg ctactatatc ttcttttgtt atggttccat
     1381 tttcatatag agttccatct aaatcacaaa atatatgttt tatcataaat taaaagcctc
     1441 caaaaacaat tttatatttt actaatactt taatcaaaat aacttaaagc ttttctaatt
     1501 gtattctcaa ctcattaagc aaatctctag tagctcttaa tcttatcttt cttctatccc
     1561 cattaaatat atttttcttt actattgttt ttccatttat atatattgca gtataaacca
     1621 gtcccacagg cttctctttg gttcctccac caggtccagc tactccagtt gtagatatac
     1681 ctatattagc tttaagtcct ttagcaacgc cctcagccat ttctatagct gtttgctcac
     1741 ttactgctcc aaactcttcc aaagtttctc tttttactcc taatcttttc attttagaat
     1801 cattagaata tgttacacaa ccttcaagaa atacagagga tatacctgga tagtttataa
     1861 gagatgaaga aaccaaacct ccagtacaag attcagccac tgctattgtt agattttttt
     1921 ctacaagtag cttagaaaca gcatcttcta aagcagtctc cccttcagat atggaaatat
     1981 ctccttcagt atatacatat ttaccaactc tactttttat tttttcttct actggtttta
     2041 ttaatttaaa agcttctctt tcattttcag cctttgcagt tattcttatt gtaacttcta
     2101 attcttttgc atataaagca actgttggat tagtctgctc attaattata tcgagaattt
     2161 ctaattctag atttgactca cctattccat ataatcttaa agtcttagat actagcattt
     2221 catttgtgaa ttgttgtaag taaggtttga cactctcatt aaacatagct ttcatttctc
     2281 ttggaggtcc aggcaatact attataaatt taccatcttt ttttaaaatt gcccctggag
     2341 ctgtaccatt attatttttt agtactatag catcttttgg aaagtacact tgtttcatat
     2401 tattttcggc catttttttc cctgttttaa caaagaaaga ttccaaaacc tctagtgaag
     2461 gtttatgtag ttctaatttc tgaccaaagt attctgcagc agtttctttt gtcatatcat
     2521 caccagttgg cccaagacca cctgtagtaa ttacaaaatc acttctcttt aagctttcat
     2581 caaaacattc taaaagtctt tgtttgttat ctccaacagt actctgatgg tatacttcta
     2641 ttcccaatga tgcaagttct ttagctaaaa attgagaatt tgtatttact atatctccca
     2701 ataatatttc tgttcctaca cttattattt cagctttcaa aatttatcac tccttgattt
     2761 atagccatta agactagtct taagttcata acaataattt tagcaaaaat aataacataa
     2821 ataaagttaa aaaatcattt aaacatatct ttaaactaga ttattattct aaaaccacac
     2881 ataatataaa cttcgtatat agtggtactt tcatcctcaa ctgttaattc atataattaa
     2941 gtatgtaaat aaaaagttta ctttaaaata ataaaaatta aaataggagt gtataatatg
     3001 aaaatagcag ttataatggg tggaatatca tcagagagag aagtttcttt aaattcagga
     3061 aaagagatat ataataacct tgataaaaat aaatatgaag ttgtaaaagt aataatagat
     3121 gataaaaaag atatatttac aaagatacca gaagatatag attttgcaat acttgcttta
     3181 catggaaaat ttggtgaaga tggatgtata caatcaatac ttgagactat ggatatacca
     3241 tactctggat gtggacctct ttgcagtggg atgtgtatgg ataaaaatat aactaaaaaa
     3301 atgcttagag attcaaacct acctactgct ccatgggttt tagtaaaatc agttgatgaa
     3361 atagactatg atgaaataga taatataggt tatccagtat ttataaaacc aaactcagga
     3421 ggttcaagtg tagccacatt ctttattcat tctaaggatg aagttgaaga agcagttaga
     3481 aaaggtctgg aagttgatga atttgtaatg attgaaaaat atataccagg tggagaatat
     3541 acttcattca tattaaatgg agaagtgttc cctacaatat ctataaaatc tgattcagga
     3601 ttctttgatt atgaagctaa gtactcagtt gaaaaaggtg caaaggaaga agttgtttac
     3661 ttagatgaag aacttcaaaa aagagtaaac gaaatttctg agacttgttg gaaaattttt
     3721 aattgcaaag catatgtaag agttgatatg attataagtg aaggtattcc atatgttctt
     3781 gaactaaata ctcttcctgg aatgactcaa actagtttaa ttcctagaag tgctgctgct
     3841 agaggtatta agtacagtga actattagat aaacttatag aatactcttt aaattagatg
     3901 aaataatttg aggtggtaat ttgatatttt caaatctaaa actaaatgat aatgaaccaa
     3961 tttatataca acttaaaaat tatataagtg atatgatatc aaaaggacta attccagaca
     4021 atagtaagct cccttctaca agggagctta gtcagctttt acaagttagc cgaaattctg
     4081 tagttttaac ttatgaagaa ttaaaatcag aaggattaat ctactctatt tcaggaaaag
     4141 gtacatttgt taaatcaaag aataagtctt caaatactac atggtcatta aactgggatt
     4201 gtttagagaa tacatactct aaaaaagcca atgaattgga cattataaaa agtgaaattc
     4261 cttggagctc tgacctgatt tcctttaaaa gtatttctcc agatggagat ttattcgaca
     4321 tagaagaatt aaaaaaatcc tttttaaata gaatttcact agaaggacat aaattactca
     4381 actatggata tgcacaaggt tataagcccc ttattgacta tttactagaa tatatgacaa
     4441 ataagggtgc tgatataagt aataaagata tactaataac aaatggattc acagaaggct
     4501 ttgatattat tatgtcatct tttacccaag aaaaagacta cataatatgt gaaaatccta
     4561 ctcataatac tgctataaaa attatgaaat ctcataacct caatatctta ggtgtagata
     4621 taaatgaaga tggtttggac ttcaatatgc tagaagacaa tttagttaag tataaaaata
     4681 aaataaagtt tgcatacatt acaccctctt atcacaatcc tacaggaatt gtaatgactc
     4741 ctgaaaatag gtacaaattc tataatctta tgaaaaaata caatatagca attatagaag
     4801 atggatttaa tgaagaatta ctctacaata gttctcatat atttcctatc tgttccctag
     4861 ataatatgaa taatggggtc gtatatattg gtagtttttc aaaaatttta tttcctggaa
     4921 tgagaattgg atggatattt gcagataaaa aagtaatcaa taagctagaa agtgtaaaaa
     4981 ggtgcagaaa catacatgta tcctttttgg accaaggaat tttatttgat tatttaagta
     5041 atggaggttt tgaaaaatac attaaaaaaa tacgtaaatt ctatggggac aaatttaact
     5101 ttgcatataa gtgtataaaa aaatatataa aaacagaata tatcttgggt gatggtggtt
     5161 tgcatatatt tataaaacta aaaaatatag atactagagt tttattagaa aaatgttatg
     5221 aaaaaaatgt tatatttatg cctggagatt tattttttac agataactct ggttttgata
     5281 ctttaagact cggattttct aggttgtctt ttgaagacat tgaaattgga ataaaaataa
     5341 ttggagaaac tataaatgaa atgttctagg ttaactaaat attgttagcc tagtttattt
     5401 ttatgaggta taatatatat agtttatatg aatctaatac tgggggataa tatgagtaaa
     5461 aagtcaaaac gaagaaaaat taataaatta aaattaacga ttgtaataac tgtattattt
     5521 cttgctttat tatctatata tgagatactg ttctctaatc ccaacaaaaa aggtaatata
     5581 aagagtcaag aaactatgca aaataaaaat aatacaacag atgattctaa aaataatcaa
     5641 agctctacca aaaagcagga agataataat atcaagccta ctttttcata ttcttcaata
     5701 ccagataata taaaaaataa aatgattggt aaatctatgc ctactgatga acctataagc
     5761 tttgatagtc tatcttattt aaaacttaca tattatggat ttgatgaaaa aactcatcaa
     5821 ggagaaatga tagtaaacag tgaactagct ccagaagtag tggatatttt taaggaatta
     5881 tacgaaaaaa aatatccaat tgaaaaaatt aagttaatag atgactatga tgctgtagat
     5941 gaaaaatcta tgtctgataa taatacctca tcattttgtt atagaactat agctggtaca
     6001 aatgtagttt caaatcatgg taaaggacgt gctatagata taaacccatt acagaatcct
     6061 caagtttctg gaaatgatgt tacacctaaa gtaagtacag tttatgctga taggtctagc
     6121 acaaaatttg gaatgataaa aaaaggtgat gattgttata atgcttttgt aagcagaggt
     6181 tggagttggg gtggctattg gaaaaaccca gattatcaac attttgaaaa ataatagatt
     6241 agaaataaag gtgaattata tgaatttaat gacattggaa aatataagta aaagctattc
     6301 tgaaaaaaaa ttactagaaa atatatctct tggaataaat gaaggggaaa aaataggact
     6361 tattggtgta aatggtactg gtaaatctac cttactcaaa attattgcag gagttgaaga
     6421 aagtgagact ggtaatataa caaaaactaa tggtattagg atagaatatc tacctcaaaa
     6481 tccaacttac aatgaaaatt caactgtact tgaacaagta tttaatggaa cttctgaaga
     6541 attaaagcta cttggtgaat atcaagaaat cctagaaaaa cttaattcaa actttacaga
     6601 ggaattaaat aaaaaattat tatcagtaca cgaaaaaata gatgccttaa atctttggga
     6661 tttagaaagt gaagctaagt cagtacttac aaagcttggt ataaatgact ttaatcaaaa
     6721 aatagcagaa ctttctggtg ggcaaagaaa gagagtctct ctggcatcag cccttattac
     6781 accttgtgag tttttgatac tagatgagcc tacaaaccat ctagacaatg atactataga
     6841 ttggcttgag gaatatctta attccagaaa aggctcattg cttatgataa ctcatgatag
     6901 atacttttta gaccgtgtta caaatagaat tattgaactg gacaaaggta gattattcag
     6961 ttatgatggg aattattcaa tatttttgga aaagaaaatg gaaagacttt cgctagaagc
     7021 aagtattgaa gataaaagac aaaatttaat aagaacagag cttgcgtggg taagacgagg
     7081 agctcaggct cgtagcacta aacaaaaagc tcgtttacaa agatttgatg aacttataaa
     7141 tagagattct tttaaacctg atgaaaatct tgatatatct gttgcatcaa gtagacttgg
     7201 aaataaaata atagaaatac acaatatatc taaatcattt gaagaaaata ctgttataga
     7261 taatttagaa tacactttaa ctcgtacaga tagaattggt ataataggca aaaatggtat
     7321 gggtaaatct acccttataa atatacttaa tggagaaata gagcctgata gtggatatat
     7381 ttcaattgga gaaacggtta aaataggttg tttttctcaa gacgactctc atatgaatat
     7441 tgatatgagg gctatagact atgtaaaaga agctagtgac tatatagaaa cttcagatgg
     7501 tactaggatt acagcttctc aaatgtgtga aagattcctt tttaatggta ctatgcaata
     7561 tactttgata ggtaaattat ctggtggtga aagaagaaga ctacatcttc ttaaaatcct
     7621 tatgacagct cctaatgtgc ttcttttaga tgaacctaca aatgatttag atattgaaac
     7681 gcttaaaata ttagaagaat acctagacga atttaaaggt gtagtagtta cagtatcaca
     7741 tgatagatat ttcttagata gaatttgtaa taaaatattt gcatatgaag gtaatggaaa
     7801 aattcatatc tatacaggta attacagtga ttatcttatt tatagagaga ttcaaggaat
     7861 tgaatttgaa gaatctaaaa gggaatcagt taaaaaagat gactctacta ccccaaaaaa
     7921 agaaaaacct aaaaatgata aaaagcttaa gttctcatat aatgaacaaa gggagtttga
     7981 aaatattgac tctgatatag aaaaactcga atctaaaatt gcaacacttg atgaaagtac
     8041 tgcaaaattt tcaactgatt ttactaaact ccaagaaatt ttagatgaga aatctaaact
     8101 agaaaaagaa cttgaataca aatatgaaag atgggaatat ttaaataatc tagctgatga
     8161 aatagctaat aataagtaat attaaaataa tcttaagttt aataagtttt gctttaaatc
     8221 tgtaatttat aaatatttta aaggaagtgt ctcaaaatga actttttagt tttatgagac
     8281 actctttttt atgagaaatc aaatatattt tcatcatttc aacaatacaa aagaggctta
     8341 tctctatttt gagacagcct ctttcataat taccttctaa tttatatgct ctaatatatc
     8401 tataataatt cttattatat ctatatatcc agtctttcaa tatagtgaca attaacttca
     8461 atatgattta aaaatactaa caacctttca taaagtggat gagattcttc tccatattta
     8521 gcttctaggt tcttgcctat ttctctaaca gtcctttgac catcaatttg taaaaaaaca
     8581 aaactggaat attcatctag ttctattttt ttgtatttag gtattctagc ttttaacttt
     8641 ctaaaaaact tttgaatttt atggtcctgt ttttcaagta tagtaacaac acgattttca
     8701 gctacttcat attcaagtcc atcagtaatt ttaaatacaa tatctaaaac atcattactc
     8761 ttgctcatta ttttttactt tagataaaac aataggtaat gctgtaagca caactaaaac
     8821 tactaataaa gcaattgcca ttgaatttgt tgcagcaaat ccacttggta cctttggagt
     8881 tacaactcca gttacttgta atattatacc tattaaacca atgatagaac caccagcaac
     8941 aagaccagaa gataaactta taccattaga aactttaact tctttttctt tttcagtttt
     9001 agatactaat tcaacaaata aacgaattaa tgctccaact aatattatag aagtagttgc
     9061 aattggtaaa tagaatccta tagctatagt cattattggt aaatttaatg catatagaac
     9121 tattgccata aatactccaa caataatcat aacccaaggt aaactacctg acataatacc
     9181 agaagttaat gttgacatta agtttgcttg tggtaatgca aattgtacat tatcaccagt
     9241 tgctctaagt tggtcagaaa gtaataaaat tactcctaca acaactataa caccaactat
     9301 acttgcaatt gtaaagtaac gttgcatttc gtttttgcta ccaccaataa tataagttac
     9361 tttttgagat tgagtataac ctccagctat agcaatagca acaactataa atgaaccaaa
     9421 taaaagtaat gatttattag cttctaaatc agtccatccc attataacaa acactaatgt
     9481 tacaataact agagaagcta tagtcatacc agaaactgga agatttgatg ttccgattgt
     9541 tcctgttaaa cgaccagcta ctatgacaaa taataaagat aataaaagag atatgatagc
     9601 tccaataatt gccataacaa tgttaccaga aattaagaat gctgctaaaa atccaataac
     9661 tactccacct aataaaagta tcatttgtat agatgaacct tcttctcctt ctccagcact
     9721 tgatttagct ttaagtgttt cttttataga agcgattata gttggtataa gttttattgc
     9781 accaataata cctccacaaa gcatcatacc agcacctata tattttacat aactactaga
     9841 tattgctcca acatccattt ggttaagagg cattgcagga ttattccata ccatagctcc
     9901 atctttagcc atatctgtaa agtatcctat taaaggtgct ataccaaagt ttgataaaat
     9961 acttcctgca aacattgtta aagaaacttc aagccctact ataaatccaa ttcctaaaag
    10021 tagaggattt acttcaatat caaatttcca tttgtagaat ttgcttccta caagactcat
    10081 tacattgttt gctacattta agaatgaacc agttaataca gtaataaatc cacttattac
    10141 aaatccaatt cccatgtact taattgaatc tccaccagcc tctgaagcaa caagtgtctc
    10201 agatatagcc attgattctg ggtacataag tttaccatgt tcttcaacta ttagatagtt
    10261 gtgaactata gcagcaatcc ctataccaaa taaaactcca ccaactccaa cagctaagcc
    10321 ttctaagaat gtaatttggc ttccaattaa tataactgct ggtaatacga aaataaatcc
    10381 acttgcaaca gattctccac cacttgacat accttggatt aagtttttac caagaatacc
    10441 ctttgaacga gcaaatgctc caacaaaagc agaacctatt atagctcctg gaatacctgc
    10501 ggcaactgta agaccggctt tcattccaga gtaagttgta gatgcggcaa aaatggcagc
    10561 taaaataata ccgataatca gtacagctac atttccaccg gttctggact tatctgtgat
    10621 atatggaacg taatcttttc cactaacctc accataagca cccttaggta attttttgtt
    10681 cataaaattt tcaccccttt tcaatttgta tatattataa catatataca tataaaactt
    10741 aactttttat tattttacaa atagcgagtc aaaactacta aaatttaacc ctatattatt
    10801 aatttctact tttagatatt tttatgctaa ttttcttttt atttttcaat tctactaaaa
    10861 actattaaga tataaaataa aaaaattttt taatgtaaaa ttaattttgt aaaaaaaatg
    10921 ctttaaaaca cggaaaagta taaaaaatac aataatcatt cttataaata aattttcacc
    10981 atagtaaata tatacagaaa attcttgttt ttatgcaaat tgattatttt ataaaaataa
    11041 gtgtcataaa gaaatgtctg aaatcacttt atgacaccta tcataataaa aaaattattc
    11101 tggtaatgct cttttattta cttttccgat tggtgtaact ggtatcttat ctatcctcat
    11161 tattattgtt ggaatcatat atggaggtag atgctcattt aaatattctc taatagttct
    11221 ttcatttatc ttaccactat cttcctcatc aattacataa tatgcacata tataaatatc
    11281 tccatcatca tttgttctcg atatacaaac aacactttta atttctgtaa tattatttat
    11341 tatattttca atctcatata gctctatcct aaactcatca atttttactt gattgtccat
    11401 ccttccaata taatctaaat ttccatcatc taaccatctt actaaatcac cagttctata
    11461 cattaactta ttatgttcac aagttttgta aggattctct acaaatactt gctctgttag
    11521 ccctttttta tttaaatatc ctcttgatat ttgaatgcct gatagacata gctctcctgg
    11581 tgctccaatt ggacatagtt tgttcttttt atctaataca tatgcataag tattaaatat
    11641 aggttttcct attggtattt ttccatattc tctgtccaca tgaaaagaag tcgatacaac
    11701 agtaaattcg gttggtccat actcattata tagttcataa ttacgctttc tatatccttt
    11761 taatctttct cctccagtct gtacaactct aagtgaatta ttattgcaat tttgtataaa
    11821 taaccttgca aatgctgttg gcaatgctag tatagttatg tcattttcct cacagtattc
    11881 atttactgta tattcatcaa atttagcttc ttcaggcata atatctattg atgcacctac
    11941 aagcaaataa gggaatagtt gtttcattac agcatcaaaa ctgaaactta agtatatacc
    12001 acatatatca ttttctgtag cgcttataga ttttacgcta taataacaca tatttactaa
    12061 actcttatgt tctatagcta tggtctttgg cctaccagtt gaccctgaag tagatatcgt
    12121 atatgcacaa tttgatgatg ttattttttc agttaattca tatccaaact taaacatttc
    12181 ttcatcattt atatcgagta ctatggtatt ctcatttaca accttacttt tgagttcatc
    12241 tgtagttatc actactttcg cattactatc ttcaagaagg taattcaatc tttcttctgg
    12301 attacttgtg tctattggaa aaaatgctcc ccctgatttc ataatcccta ttgctgtaat
    12361 aattatgtta atatctcttg gtaacattac agctactatg tcttcacttt ccactcctat
    12421 atgatttaaa taacctgcaa gtttatcagt tacttcatcc aattctttat atgttaactt
    12481 tctattttca tccctagcag ctatattact aggtgttacc ttaacttgct ccctaaataa
    12541 atcaacataa cttttatcat agggtacttc atattgatta ttattaaagt cttccaataa
    12601 tactttttct tcttccttac atacaaattc taaatcttct attaaaatat cattatcttc
    12661 tattatttgc tctattatat aatcaaattg atttaaaatt cttctaatag tttctctttt
    12721 atatatagat ggtttatact ttatttttat tttaagtgcc ctcttagtat atactattac
    12781 agataaatca tatccttttt tttctatgtc ataatcatat gataaatcaa aacttttagc
    12841 tataaatatt gtgtttataa gcttagtatt tagatgattt ttattctcta tatctgataa
    12901 caaatagtat ccatactcta aagacctgct ccactgcctt tttagcttcc ttacaacatc
    12961 tataaatttt tcactttgtc taactcttac tcttattgga attatattca ttgttttacc
    13021 aaaaactaca tcatcttcat atatatactt ttgaagtata aggccatata tagtttctat
    13081 gatagagtct atgctaatat tgttcacttt agaatattga attaaccttt catgtttctc
    13141 aatattcatc ttaaaaacct catctactac ttcatcagaa caatctttat aaaaaaaatc
    13201 atttggaatt tttgctatat tcgtataatc atctaacaaa tccatccaat aattcatagc
    13261 tcgttctaaa ttaacttcat acatatcaaa attccccctt actactaagt ctatagaaaa
    13321 aattcttttg gaattttatt ttcacaatat ataaatatat taataataca tatgcaagct
    13381 ctaaaaacat gagcttgcat aaaattacac ttattatgac tactaaattc gtaatat
//
LOCUS       DAEOSP010000108        13380 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.108, whole genome shotgun sequence.
ACCESSION   DAEOSP010000108 DAEOSP010000000
VERSION     DAEOSP010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13380
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.108"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            163..1476
                     /locus_tag="KRH54_002336"
     CDS             163..1476
                     /locus_tag="KRH54_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG8579993.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAVGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            1648..2823
                     /locus_tag="KRH54_002337"
     CDS             1648..2823
                     /locus_tag="KRH54_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBG8579994.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            2926..3945
                     /gene="hrcA"
                     /locus_tag="KRH54_002338"
     CDS             2926..3945
                     /gene="hrcA"
                     /locus_tag="KRH54_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBG8579995.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            3975..4595
                     /gene="grpE"
                     /locus_tag="KRH54_002339"
     CDS             3975..4595
                     /gene="grpE"
                     /locus_tag="KRH54_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBG8579996.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            4687..6534
                     /gene="dnaK"
                     /locus_tag="KRH54_002340"
     CDS             4687..6534
                     /gene="dnaK"
                     /locus_tag="KRH54_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBG8579997.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            6745..7899
                     /gene="dnaJ"
                     /locus_tag="KRH54_002341"
     CDS             6745..7899
                     /gene="dnaJ"
                     /locus_tag="KRH54_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBG8579998.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            8865..9800
                     /locus_tag="KRH54_002342"
     CDS             8865..9800
                     /locus_tag="KRH54_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBG8579999.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            9836..11209
                     /locus_tag="KRH54_002343"
     CDS             9836..11209
                     /locus_tag="KRH54_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8580000.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLIFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(11397..12164)
                     /locus_tag="KRH54_002344"
     CDS             complement(11397..12164)
                     /locus_tag="KRH54_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8580001.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGKLDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(12194..13303)
                     /gene="ugpC"
                     /locus_tag="KRH54_002345"
     CDS             complement(12194..13303)
                     /gene="ugpC"
                     /locus_tag="KRH54_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBG8580002.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
BASE COUNT         5141 a         1515 c         2353 g         4371 t
ORIGIN      
        1 tttttagaga atattttaga agttacgatt gtatgatttt taaatgcata ggcatttact
       61 ctaggactat agactaatat aaaaaaaaac tttgggaata ctaaaaccat aaatttataa
      121 aattaatatt ttatatactg taaaacagtg aggagggaca gtatgcatac tcaactacaa
      181 aagactattg gtctttcagc agctctttct actgttgtag gtatggttat aggttcggga
      241 gtttttttta agccacaagc aatatacact acaactaatg gagctccagg tctaggtatt
      301 atagcgtggc ttttaggtgg ttttataaca ataactgctg ggcttacagc tacagaaatt
      361 tcagctgcta taccaaaaac tggtggtatg atgatttata tcgaagaaat atatggagaa
      421 aagttaggtt ttttaactgg atggatgcaa actgtattat ttttcccagg tacatcagca
      481 gcattaggag taatatttgc tcaacaagca tctgaacttt taggaatgag cccaaataat
      541 atggcaaatg tacttcctat agcagttgga gttatactat ttttagcatt attaaatata
      601 ataggttcat ccttaggagg aaaagttcag acagtagcca ctattggaaa gatgatacct
      661 ttaattttaa taatagtctt tggatttatt aaaggtcaaa gtagtgaagt attaaatcca
      721 tttgtaggag atggtgtaaa tgcatcaaat gcacttggac aagtattaat agctactttg
      781 tttgcatatg atggttggat aaatgttgga gccatatcag gagaaatgaa gtctccagag
      841 aaagatttac caagagcaat agttggtggc ttatcattag ttatggcagt ttatattata
      901 ataaatatag cttatctttg ggtagtacca gcaagtgaat tagcaacagt tacttcccca
      961 gctacattag tagcaacaag attgtttgga aatataggtg gaaaagtaat aactgtgggt
     1021 atattaatat cagtatttgg gacattgaat ggatatttat taacaggttc aaggatacct
     1081 tatacattag ctgaaatggg aactttacca gcatctaaaa ctcttttaaa agtaaattct
     1141 ggaggttctc ctgtaaattc aatactactt ataactgtgt tagcctgtgt atatgcatta
     1201 tctggtcaat ttaatctttt gacagactta acaatatttt caatatgggt attttatgta
     1261 ttgactttta taggtgttat gaggcttaga agacaaaagc cagatttaca cagaccatat
     1321 aaagtccctt tatatccaat aataccaatt atagctatat taggtggact gtttgtaata
     1381 ataaatcaaa tattgacttc aacagtcata tctttaggtg ggatatttat aacactatta
     1441 ggtttaccag tttattatta tatgaaaaaa agataactac ttaatttaag ttagataaat
     1501 ttgggtaaag gtgaaaatta acttttatcc aaatttttta tactgttata caatataagt
     1561 attatatatt acttgatgtg ctattttgtt tgatttataa tttagtaatt gatgttttaa
     1621 gataatttaa gaaatgagga gtgaaagatg ctaggacttt atgtacatat tccattttgt
     1681 gtgaaaaaat gtaagtattg tgattttaat tcttataaaa tggacataga ttcaaaaaaa
     1741 agatatatag aagacttaaa aatagaaatg gaattatata gtaataaatt atataaagat
     1801 aataaatata aaaataaaga atgctgtagt ctaaataaaa atgacaaaat aacgagtata
     1861 tttgtagggg gaggtacacc tagtatacta acctctgatg aaataagaga agtatttata
     1921 agtataaaag aaatgtttga tatagatgag aatgcagaaa taactataga gtgtaatcct
     1981 gggacattaa cattagaaaa attaaaaact atgaaagaga ttggcataaa tagacttagt
     2041 atagggctac aagctattca agaaaagcat ttgaacttta taggaagaat acatacttat
     2101 gaagaatttg agaaaaacta taaagatgca ttaagtgtag gttttaaaaa tattaatata
     2161 gatttaatgt acagtcttcc aaatcaaact ctatgtgatt ggaaggaaac tcttgaaaaa
     2221 gttgtacact taaatccaac acatatatca gcttattccc ttatactaga agaaggaact
     2281 gaactataca atatgtatga aagtaataaa tttgaactta tagatgaaaa tgtagatata
     2341 gaaatgtatg agtatacaat aaactactta aagtcaaaag gatataatca atatgaaata
     2401 tcaaactact caaaagaagg ctataattgt gagcataata ttttatattg ggaatgtgaa
     2461 cattatatag ggattggagc aggagcttct ggctatatta atgagaacag atacaataat
     2521 gtagaatctt tagaagatta tcatttaagt ttggtaaaaa gagaaaaacc aatacaagaa
     2581 aatgaaattc tttctgagaa agatatgata gaagaaaaga tatttatggg gcttagaatg
     2641 aataaaggca taaaatttga agattttaaa aaaaaatttg gaatagattt tagagagaag
     2701 tataataagc aaatagaaat gctattagct agaaagctta taaatcagag ctttgaaggt
     2761 atacaattaa ctcaaaaagg aagagaaata tctaatagtg tatttataga atttatggaa
     2821 taatttttaa ggtgttgaca aaataaattt ataatggtat ataatatata taatcttttt
     2881 agcactcgaa cttagtgagt gctaacagct aggaggtata ataatatgga attaaatgaa
     2941 agaaaattaa atatcctcaa agctatagtt aaagattata tagaaacagc tgaagctata
     3001 ggttctagaa caatatctaa aagacatgat ttaggtgtta gtgctgctac cataagaaat
     3061 gaaatggctg accttgaaga attagggtat ttaattcagc ctcatacttc tgcgggtaga
     3121 gttccatctg aaaaaggcta taagttatat gtaaactctc ttatgagtaa aagcgaattg
     3181 gatgataatg ataagattct tattgaacaa tgtatgaatc acaatataaa tcatataaaa
     3241 gaattgattc atgaaacttc taagctatta tcgcaactaa caaactatac aactgtagca
     3301 gttactaaga gtttaataaa tcaaagtgtt ataaagcaca tacagttggt tgctatgaat
     3361 gacaataata ttttacttat agttgtaaca gataaagggg acttaaaaaa agctaacctt
     3421 acaactaatg tatatttaga tcaatctaag ttaaatttga tttctgataa tcttacaagg
     3481 aagctcttag gtaaaagtat aacagatttg gatgataatc taattgcttt tattaaatac
     3541 gaaataagtg agtattctgg gcttatagat gagcttttaa atgctttgaa ctcaaatatg
     3601 aaagaagaag atttttctct atcattaaat ggagctacta acatatttag ttatccagaa
     3661 tttaatgatg tattaaaagc aaagtcattt ttaaatatgt tggaaaaaaa ggaaacaata
     3721 gctgacataa ttaaatcaaa gggaatacaa aaagacaatc taaatataat aattggtagt
     3781 gataatgatt gtgaactggc acaggattgt agtatagtta cagcaactta taatgtagat
     3841 agagacttgg ttgggcgaat aagctttata ggccctacca gaatggatta tgctagaatt
     3901 tactctataa taaattatat gagtttatta attaatagaa agtaaagaaa ttttaaattg
     3961 cgaggtgtaa agtcttggat aaaaaaaatg agcaacagga agtaagagaa gaaaatgata
     4021 cttctattaa ccaagaatcg gaaactcaag tagagttaga agaggaagtt gttaatgaag
     4081 aatgtgaaac atcaagtgaa aaaactgatg aaaaagaagt tgatgatgaa aatgtaacag
     4141 atataaattc taagttagca gaaaaaaaat tgcaggatga gttggatgaa ttaaatgata
     4201 aatatcaaag acttcaagcc gaatatgcaa actacagaag aagaactcaa caagagaaag
     4261 agacaatagg cgtatttgca aatgaaaaaa taataactga attgatacca gttatagata
     4321 gtatggaaag agccttagat gcatgtgaag ataaggaaga tactatgtat aagggtataa
     4381 gccttgtaca taaacaatta atagacactt tagtaaaatt tggagtagaa gaaatagaag
     4441 cagaatctaa ggaatttgac cctaatctac atctagcagt tatgcaagaa agtgtagatg
     4501 gagttgaagc aaatcaaata gtaatggtac ttcaaaaagg atataagcta ggaactaaag
     4561 ttgtaagacc ttcaatggtt aaagtttctt gctaagtata aattaaaggt taaattattt
     4621 aacctaatat aaaatattaa aataagtaaa acttaattat ataattactc gaggaggatt
     4681 ttagtcatgg gaaaaataat aggaatagat ttaggaacaa caaattcatg tgttgcagtg
     4741 ttagaaggtg gagaagcaca aataatagca aatagtgaag gtatgagaac tactccatca
     4801 gtagtagcat ttacaaaaga tggtgaaaga atagttggag aacctgcaaa aagacaagca
     4861 gttacaaatg cagataaaac aataacttct ataaaaactc atatgggaac tgattataaa
     4921 gtaaatatag atggaaaatc atatactcca caagaaatat cagcaataat attacaaaaa
     4981 ttaaaatcag atgcagaaag ttacttagga caaacagtaa cagaagcagt tataacagta
     5041 ccagcttact ttacagatgc tcaaagacaa gcaactaaag atgctggtag aatagcaggg
     5101 cttgatgtta agagaataat aaatgagcca acagcagcag ctcttgctta tggtatggat
     5161 aaattagacc aagaaaagaa aatattagta tttgacttag gtggaggaac ttttgacgta
     5221 tctatacttg aaataggaga tggaactttt gaagttttag ctacagctgg taacaataga
     5281 ttaggtggag atgactttga ccaaatagta atagattatt tagcagagga atttaaaaaa
     5341 gctgaaggtg tagatttaag aaatgataaa atggctcttc aaagattaaa agaagcagca
     5401 gaaaaggcta agaaagaatt atcatcaaca atgagttcaa acataaactt acctttcata
     5461 actgctacag cagaaggacc aaaacactta aatatagatt tatcaagagc taaatttgaa
     5521 gaattaacta gaggtttagt tgaaaaaact atggaaccaa ctaagacagc tttacaagat
     5581 gcaggacttt ctacaggtga tatagatgat gtattattag taggtggttc tacaagaata
     5641 ccagcagtac aagaagctgt taagaagttt ataggaaaag aacctcataa aggtataaac
     5701 ccagatgagt gtgttgcagc aggtgcttct atacaagctg gagttttagc aggggatgtt
     5761 aaagacttat tattactaga tgttactcca ttatctcttg gtatagaaac tatgggtaat
     5821 gtaatgacta agataataga aagaaatact acaataccaa ctaagaagtc tcaaatattc
     5881 tcaactgctg ctgataacca aacagctgtt gatatacatg ttcttcaagg tgaaagaagt
     5941 atggcttatg acaatacaac tttaggaaga ttccaattaa cagatatacc accagcacaa
     6001 agaggaatac ctcaaataga agttactttt gatatagatg caaatggtat agttaatgtt
     6061 tcagctaaag atttaggtac aggaaaagag caaaaaataa ctataacatc aaacactaat
     6121 ttatcagaag cagaaataga gcaaaagata aaagaagcag aaatgaatgc agaagctgac
     6181 aaacaaaaga aagaaaaaat agaagcattc aatcaagctg aatctactat atatcaaact
     6241 gaaaaaactt taaatgaact tggagataaa ataagttcag gtgaaaaaga ggatatagaa
     6301 aaagctatag cagatttaaa agctgtaaaa gataatcaag atgctacagc agaagaactt
     6361 aagaaagcta cagatgaagt aatgactaag ttccaaaaag tatctcaaga aatgtaccaa
     6421 aaagcagctc aagaacaaca agcggcacaa ggtgcagaac aagcacaaga caatggacct
     6481 aaagatgaca atgtagtaga tgctgacttt aaagaagtag atgaagataa ataagctata
     6541 taatatgtaa cactaaatga taattggaac atatatgatt tgtgaaaatc atatagatgt
     6601 taaagatata tggacaatat acatcataaa gaatataata tatcatagat tatataactt
     6661 aaagtagaat ataagaatta atgatagctt gtagtttatt attctacaag ctatcgtttt
     6721 gtaaaatgaa ggtggtgtca atttttgagt acaaaaaggg attattatga agtcttaggt
     6781 atcagtaaag gtgcagaagc tcaagagata aaaaaagctt atagaaaatt agcaatgaag
     6841 taccatccag atagaaatcc tggagataaa gaggcggaag aaaaatttaa agagataaat
     6901 gaagcttatg aagttctatc tgatgataca aagagaaaaa cttatgacca gtttggacat
     6961 gatgggctaa atggtcaagg cggttttggt ggtcaaggag gatttggcgg tcaaggcttt
     7021 ggtggttttg aagatatgtt cggagatata tttggtgaca tgtttggagg aagcttcgga
     7081 ggtggaagag caagaagaag aggacctcaa cgtggtgcag atataagaca gagtgttact
     7141 atatcttttg aagaagctgc atttggtaaa aagatgtcta taaaagtaaa tagaagtgaa
     7201 gagtgtgaag aatgtaatgg aactggagca aaaccaggaa catcaaagaa aacttgttca
     7261 acttgtaatg gaactggtca agttagaact gttcaaagaa ctccatttgg taatatagca
     7321 agttcaagac catgtagtgc ttgtaatgga actggagaag tgattgaatc tccatgtagt
     7381 aaatgtcatg gaactggaaa tacaagaaaa gttaagacta tagaagtaga tatacctgct
     7441 ggtatagatg atggtcaaat gataaaactt tctggtcaag gtgaagtagg agaaaaaggt
     7501 gctccaagag gagacctata tattgtagtt aatgttaagt cacatccact atttactaga
     7561 gatggaaatg atatttattt tgaaatgcca attacatttg ttcaagcaac tttaggtgat
     7621 gagatagaag tacctacttt ggatggaaaa gttaaatata gtgtgccaga ggggacacaa
     7681 actggaactg tatttagact taaagagaaa ggaatcccta gaattagagg taattcaaga
     7741 ggagaccaat atgttaaggt tgtagttgaa attcctaaaa aattaaacga taagcaaaaa
     7801 gaactattga gagaatttgc gaaggagtgt gggtcgaacg tccacgaaaa gaagaaaacc
     7861 ttcggacaaa agatagaaga tatgtttaag aaaaagtagt taaagtagcc aatatataat
     7921 catattttta aaatcttagt tatttataag attgtcaaag gagatattat gatttttata
     7981 ttggctattt ttgtataaaa aataaatgtg cataaaataa aactgaatat aaataatttt
     8041 ctgaaaaata cttgtcctaa aatatataat gtgctataat ataatcaaat aaaagaaagg
     8101 ggtgattcca gtgggaacgg atgtggcact gccaaatgat gaagaaaact ttattgtaat
     8161 atacaaaata aaattagata cttttagtgc tagattaagt actggaaaaa ctcatgagct
     8221 gggataatta ttgtaattac ctagactcaa acttttagta gtaaaatagt aatattatat
     8281 agacaaggta tctaattttt aaatcttttg tatgaattaa aatttttata aagggtatat
     8341 ctgattagaa tgtaattagg gaaacctagt tgtgaattac ttttagtata tacatgtgta
     8401 aagaaatatg tatatggtat tggttattgt gctagatgtg tatgtaaaga aatatgtgtc
     8461 tagttgggta tatgtaaaga aatatactta taatctatga aaaattttta gtataaattg
     8521 ctaaaacatt gagattgttt tttatagaaa gttagtttat accaactaaa aatgtgctta
     8581 agatttgttt gatgtttaat tataaataag gttgtagata aaaaaataag taaagacaaa
     8641 ggtttaaaat acttgcttta aatatagttt gttttgaaca cttgccaaat taaaataatt
     8701 aataaaatag acatcttctc atagtaaaat atactgtgag aggatgtttt tgctttgttc
     8761 ataaactttt atgaaaaatt tgcatagtgt cacattttaa tatactatat atataataaa
     8821 atatagataa aattaaatga ttcaaaagag aaaggtgaaa agagatgtat tatataggtg
     8881 tagacatagg aggaacaggt atacaagcag gagtagttga taactatggg aaaataattt
     8941 ttaggtcaga gtgtaaaaca gtaattgaaa aaggatttga aggaatactt aatgacataa
     9001 aaataatgat ttataagttg ttggaagata ataaacttac tatgagtgac ataaaatcta
     9061 ttggatttgg tgttccaggt tttataaata aagaaggatt agtaacttgt gtaaatttaa
     9121 aatggaataa aaaagctttt aataaagagt taaaaagaag atttccagat gttgaaattc
     9181 atggagaaaa tgatgcaaca gtagcagcac ttggagaggc aaagtttgga agtatgaaag
     9241 gtgctaatgt tggagtatta tatactttag gaactggtgt aggtggagga atagtaatta
     9301 atcaaaaagt cttttctgga gcacatggat tgggttcaga aataggtcat caaattatag
     9361 gagaaaacta ttttaattgt aattgtggaa ataatggttg tgtagaaact ttttgttcag
     9421 caactgcaat tataaagtat tctcaaaaac tgatagaaga aggagaaaag agtaggattt
     9481 tagatttggc agagggaaat ttagaaaatg taaatgcaaa gatggtgttt gatgcatata
     9541 gagaaaatga tttagtggca attaaagtaa taaatagatt taaagagtat ttagcaaaaa
     9601 cttttgcaaa cacaataaat tctttagacc cagaaattat atctatagga ggaggcattt
     9661 ctaaatctag cgatataata cttgatggaa tagaagattt ggtaagaaaa tttgtattat
     9721 acaaaacaga agatatagct acaattacat gtgcaacttt aggttcagac gcaggaatta
     9781 taggagcagc ttttttataa tgtaaatgaa ctgcttattg gaggcgtgat gatatatggg
     9841 taaaacaaat gctagaagaa cgcttttttt gatagcaata ggttcaggaa ctatgctgaa
     9901 tcctcttaat tcttctatga tttcacttgc tttacatagt atacaaaatg attttcatat
     9961 tagttttaca actgtatctt ggattgtttc agcattttac ctagctagtg ctattgcaca
    10021 accagtttca ggtaagatag gagatttaat aggtagaaag gtcttatttt tgagtggttt
    10081 gatgcttgta attttatcag catccatagt accacttgtt caatcattct ttatattgat
    10141 ttttataaga gtaattcaag caattggaac aagtacccta tatccatctg gagttgctct
    10201 tatacaaaat aatataaaag aaagacaatc ttctgcatta gcagttctaa ccatttttgc
    10261 ttcaactaca gcaggattag gtccgacact tggaggatta ttgttggact taggaggatg
    10321 gcatgctata tttcttgtaa atatacctgt tgttttggtt tctatatgct tagggtattt
    10381 tttattccca aaagaagtaa aagaaaagaa atctataaaa gaaactttaa aaaatgtttt
    10441 gtcaagatta gatattattg gcatacttct attttcaatt gcaatgattt ttatattact
    10501 atttttgcta tctatgaagg agtcttttaa cttagaacaa cttatatttg gtataatact
    10561 gatgtgtctt tttatttggc atgaattgag aacaaaattg ccatttatag atattaagct
    10621 ttttgtaagt aatcctaaat tgtcaaaagt ttacttgcaa tttattatat taaatttctt
    10681 taattatatt ttgttttttg gtcttcctag ttattttcaa gatgccttac attatagtgc
    10741 aaagtcgact ggattattta tgctatttat gtctggtata ggtatattta tatctccatt
    10801 aactggaaaa tggatagata aatctggaac tagatttcct cttgttacat catcaatctt
    10861 tatgtttatt agtgcagtac ttctttcagt agtttttatc catatacctg taataggaaa
    10921 gggtattata ttatcattag caggtattag ttatggagtt ggtaatgtag ccctgcaatc
    10981 ctctatgttt gaagaaagtc ctcgtgacag tatagggaca gcttctggtt tgtttcaaac
    11041 tagtcgttat ttaggttcta ttttagcaac tgttttactt ggaatggtat ttgaagtaaa
    11101 tataacatca gaacaatttc agatattagg ttatgttatg gtggtcttag gtacaataag
    11161 ttttttatta aattttatat taaagaaaga attaagaaaa gtagaatagt ggatttatgg
    11221 atttaataag tctaataact gataagttta aaaatattgt ttgtaaaaca taactggttt
    11281 ggtatttatt aattctaagt tttgaagcat agagtactcg ataagtatac aatattttga
    11341 attaaaatta agcagaaatt tgaactagtc tttcaaatct ctgcttaatt tagtagttaa
    11401 atttttttat agagaaaata agatttgaca cttagataaa aaatcattgc attttcaaaa
    11461 ttctttaaat caaacccaag tatctcatta atcttttcca atctatatac tagcgtgttt
    11521 ctatgtaaaa acatactagt agatgtctta gaaatattta aatcattatt tataaatttt
    11581 attcctgtat atagaagttc ttcgtcaata gatttaatct tattaaaatc tattaattta
    11641 ttgagtatat tatcctcatt aattaaatct aaagttttat atctaataag gtcaaacaaa
    11701 tttaaaaact tattttcagc gttgtctttt ataatatgag gtagtaaatc agaacataac
    11761 tgtaatctaa aatcaaaatc agctttacta tctaatttac caagataaaa aatataagta
    11821 ctttgataag tttcagtttc aattatattt tgtgtagtta cattaatatc taagttatat
    11881 ttagaattta ctataaaaaa accattgtgt tgtattatat ttatatcttc aaataaatta
    11941 taaaaaatat tttttagata ttcaaagtct atatgagatt ggtaaattaa ataaaagttg
    12001 ccttttaaat tatcgagatt atttttatct agaaaatcaa taacataaat agcattattg
    12061 aaagtaacta aatggctact agcatctaac ttttggtttt catatggata gatatttatt
    12121 tttttattaa gctgtctttc taaaaaatct tttaactctt ccataaagtc tccttaaaaa
    12181 taagattata ttcttatctt atagctaaag tagtttcttt gtcaaatatg tgtactctac
    12241 tattgtcaac tgaaagtgtt aagtcatcac caacattaat tctgttttta ctgtcaaatc
    12301 taattgttat cgtattttcg cctagtttta agtgagcata agtttctgct cccataagtt
    12361 cttttatttc aattcttgat ttaaaacaag tatctggatt agatttaata aaagtatctt
    12421 ctacatgaat atcttctggt ctgataccta taacaacttc attattaata taattgtctt
    12481 ttattaaatc atagtgtttt tctttattca atttaagctt aatatgttca ttttgtgcat
    12541 aaatttcatc attttcttct attaatatta catcaataaa attcatctgt ggtgctccaa
    12601 taaatcctgc aacaaatata ttagctggat aatcgtatac atcttgtgga gttgcaatct
    12661 gctgaactac tccatccttc attactacga ttctatcagc catagtcata gcttcaactt
    12721 ggtcgtgtgt tacatagata aatgtagttc caagctgttg gtgtagctta gttatctctg
    12781 ttctcatagc agttctaagt tttgcatcta agttggatag aggttcatcc attaagaata
    12841 ccttaggatt tctaactatt gcacgtccta aggcaaccct ttgtctttgt cctccagata
    12901 atgttttagg ttttttattt aaaagaggta gtaaatctaa tattttagca gcttctttaa
    12961 cttttttgtc tatttcatct ttgggtaatt ttctaagttt aagtgcaaat gccatatttt
    13021 cgtaaacaga taaatgggga tataaagcat agttttgaaa aaccatagct atatctctat
    13081 cttttggttc tatatcattg acaagtttat ctccaatgta tagttctcct tcagagattt
    13141 cttcaagacc agcaatcatt ctaagtgtag tcgatttacc acaaccagat ggtcctacta
    13201 aaacgataaa ttctttatct ttaatgtcta tatttatatt ttttactgcg ttaaatccat
    13261 ttgagtaaag ttttgatata ttttttaaaa ttacttctga cataatatcc tccttgatat
    13321 ttaaatttta attatatagt atcaaatttt tttgttatat taaatgtgta aaatgactaa
//
LOCUS       DAEOSP010000109        13252 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.109, whole genome shotgun sequence.
ACCESSION   DAEOSP010000109 DAEOSP010000000
VERSION     DAEOSP010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.109"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(11..1015)
                     /locus_tag="KRH54_002346"
     CDS             complement(11..1015)
                     /locus_tag="KRH54_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBG8580003.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(1029..1931)
                     /locus_tag="KRH54_002347"
     CDS             complement(1029..1931)
                     /locus_tag="KRH54_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG8580004.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            complement(2126..3301)
                     /locus_tag="KRH54_002348"
     CDS             complement(2126..3301)
                     /locus_tag="KRH54_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBG8580005.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(3388..4233)
                     /locus_tag="KRH54_002349"
     CDS             complement(3388..4233)
                     /locus_tag="KRH54_002349"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBG8580006.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(4331..5128)
                     /locus_tag="KRH54_002350"
     CDS             complement(4331..5128)
                     /locus_tag="KRH54_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBG8580007.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(5147..6157)
                     /locus_tag="KRH54_002351"
     CDS             complement(5147..6157)
                     /locus_tag="KRH54_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBG8580008.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(6176..6958)
                     /locus_tag="KRH54_002352"
     CDS             complement(6176..6958)
                     /locus_tag="KRH54_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBG8580009.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(7017..8153)
                     /locus_tag="KRH54_002353"
     CDS             complement(7017..8153)
                     /locus_tag="KRH54_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBG8580010.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(8862..9329)
                     /locus_tag="KRH54_002354"
     CDS             complement(8862..9329)
                     /locus_tag="KRH54_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBG8580011.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(9408..9950)
                     /locus_tag="KRH54_002355"
     CDS             complement(9408..9950)
                     /locus_tag="KRH54_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580012.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            complement(10002..10688)
                     /locus_tag="KRH54_002356"
     CDS             complement(10002..10688)
                     /locus_tag="KRH54_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG8580013.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            complement(10688..11548)
                     /locus_tag="KRH54_002357"
     CDS             complement(10688..11548)
                     /locus_tag="KRH54_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580014.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            11683..12483
                     /locus_tag="KRH54_002358"
     CDS             11683..12483
                     /locus_tag="KRH54_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBG8580015.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            12508..13077
                     /gene="yfcE"
                     /locus_tag="KRH54_002359"
     CDS             12508..13077
                     /gene="yfcE"
                     /locus_tag="KRH54_002359"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBG8580016.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
BASE COUNT         4189 a         2425 c         1451 g         5187 t
ORIGIN      
        1 agtatataaa ttacttttct tcataaaacg taacattctt tattattata tctatagttc
       61 catcttgatt ttgttttaca gtatacttca ttgggtcttc aaaatcagtt agattccctt
      121 ttatgtcaaa accattatcc gtttttatac ttcttttttt aagctttttc tcaacccatt
      181 ttttatctat actaaaactt tcatcaagac ctttttcttc catatgctct ttaaagctat
      241 cctttaactc gtcatcttta attgaattat caacaaagtc atttatatca acttcatgtt
      301 tttccttcag tgtataattt aatatacttc ttacatcttc agcttgcttt atatcattac
      361 taagagcatt ggttatccaa ttttcagctg tatttttaaa taccttggtc ttgtacttat
      421 catcatctat ttttttagca tttaaaaatt ctgttacaaa cttagattct gacccttctt
      481 tttcagcatc cttatctaaa agccttagat gaaactcatc atttatacca cttagactaa
      541 tcaaagctcc ttgcttttgt cttaaagtct ctgggatacc aatttcattt gaaaccattt
      601 gaatattaaa tttatcatcc acaaattcaa ttgaatgagt gtacaacttt ttataatcta
      661 atttcagtat tgctacactc ttttcatctt taatagtgta taaacaaatt gccaaatcac
      721 aagattctag tgcagcatta attttcataa cgtcaaacaa gtaagatgca atttctttag
      781 aatttttcaa aaaagtactt tcatcatata ttatttgttc acaacagttt ttaattaaat
      841 tgtcattata atctttaaaa acaccttttc ttaaatcctc atcttttgca attcttttta
      901 tagctttctg gaaaaaacca tctacttctt gattaacttt gccttcaaag tcatttaata
      961 ttggaacatc actatttttg tccaaaacat gtattataaa tttatgtatt atcatatata
     1021 tcaccccatt atcttaattt cgatattaac tctttcatat cctctggtat atctgcttta
     1081 aatttcaatt tttcttttgt tcttggttgt tcaaactcta acttatatgc atgaagtgct
     1141 tgcctattta ttagctcttc atctacataa ccatacaact catcacctat tattccatga
     1201 cctatatgag ccatatgtac tcttatttga tgtgttctac cagtttctaa gcttacttct
     1261 aatacagttg cattttctag tctttctatt actttatagt gagtaacaga tgactgtcct
     1321 ctttcatcta ttatcctctt tattgaatct tctgtaggtc tataaattgg ctcatctata
     1381 gttccctcat tttcttttat aattccatca actactgtta tatacttttt ttcaacttta
     1441 ttttcactca ttgctgtaga caaagtatga tgtgcataag cattttttgc tactataact
     1501 aaccctgaag tattcatatc taacctattt acaaatctta tttttacatt ttctttttga
     1561 ttatctatat agtaactaat accatttgct attgttttat catagtggct ttttgttggg
     1621 tgaactacca taaacggagg tttattcacc ataattatat caaaatcatc ataaattatc
     1681 tgtaaattta aatcttgagg ttcaaaattt gctttttcct cgtctatttt tacttctatt
     1741 aaatcaccac tatacacctt taaactcggt tttttatata ctccatttac taaaacactc
     1801 ttttccctct tcatttttga taatgaccta actgagaaat ttagcttgtc caataaaact
     1861 tcttttaaag tcatttcttc ttcatttgta tacgatataa gattatatct ttgattctct
     1921 tttttaaaca aacttttacc tactttcttt tatatcttcc tactttttat atcttcaaaa
     1981 tcatgatatt atatatttta cactattttt gtttaataga taataattat ttttaactat
     2041 aaaaacctgt aagaataaca aatagttatt cttacaggtt aaattcataa ttaaatattt
     2101 aatttgtaaa tattataatc taaaactatc tcttaactat taaagtagtt cccattcctc
     2161 cacctataca aagtgtagca agaccagttt tagcatctct tctcttcatt tcatataaaa
     2221 gtgtagtaag tattcttgct cctgagcatc ctattggatg tcctatagct attgctccac
     2281 cattaacatt aactttgttc atatctatat ttaagtctct tattacagct acagattggg
     2341 cagcaaatgc ctcattagct tcaactaaat ctatatcttc aatagtcata ttagcagctt
     2401 ctaaagcttt tttagttgct ggaactggtc catatcccat tattttaggg tcaacaccag
     2461 ctgttccata agaaactata gttgcaagag gctctattcc tagttcttca gctttttctt
     2521 tagccattac tactagcata gcagcaccat catttattcc tgatgcatta ccagcagtaa
     2581 ctgttccatc ttttttaaat gcaggtctta acttagcaag tttctccatt gtagtgccag
     2641 gcttaatata ttcatcttta tctactacag tgtcaccttt tcttcctttt ataacaacag
     2701 gaactatttc ttcatcaaat tttccttcag cttgagcttt ttcagcttta ttttgacttg
     2761 caagagctaa ttcatcttgt tcttctctag ttatattcca ttgctctgct atgttttcag
     2821 cagtaatacc catgtgatag ttattaaata tgtctgataa tccatctttt atcattgaat
     2881 caacaaaagc agcatcaccc attcttgcac catatctcgc acttggtact aaataaggag
     2941 acatactcat gttttcagct ccaccaacta acattatatc agcatcacct aatgctataa
     3001 gttgagatgc cattgaaaca gatcttaatc cagaaccaca aactatattt atagtcatag
     3061 ctggtttttc tactggtatt cctgctccta atgctatttg tcttgctata ttttgtccaa
     3121 gacctgctgt aagtactccc cctaaaagag attcatctat catatctgga gttatgttag
     3181 ctctttttat agcttcttta gctgctgtta cccctaactc taccgctgaa actgatttaa
     3241 atgctcctcc aaaacttcct actgctgttc tagctgcact ggcaattact acttctctca
     3301 tattcattac ccccattaaa ttagattttt catatatccg aaattgtttt ttaaacaatt
     3361 ttatttaaat ttttaattcc caaattctta tttattataa tcatagaatc ctatcttagt
     3421 ttttcttcct aattgattag ctctaaccat tttagctaaa agtggatgag gtctatattt
     3481 agtatctcca aactcagtat ataaaacgtt cattatggct aaaacaacat ctaatccgat
     3541 taaatcacct aatgctagtg gtcccattgg atggtttgct cctaatttca tagcttcatc
     3601 tatttcttct ttacttgcaa caccatctgc atatatacca acagcttcat ttatcatagg
     3661 tataagtatt ctatttacta caaatccagg agattcagat acatctactg gtactttatt
     3721 gatactctta gataattcaa atactgtatc aaaagtaact tttgatgtta actgaccact
     3781 tataacttca actaatttca tcataggaac tggattaaag aaatgcattc ctataacttt
     3841 atctggtctc ttagtagaag aagctatttc tgttatagat aatgatgaag tatttgttgc
     3901 caagatagta tcttctttac ataattcatc tagtaacttg aaaacatctt tctttatatt
     3961 catgtcttct acagatgctt ctattattaa atccatatct tttaaatctt catagttagt
     4021 agttgaacta acatgactta atatttctgc ttttgtagct tcatccattt ttcccttagt
     4081 aactaactta gttaaatttt tatctaataa agctaaacat ttatctatag caccttgagt
     4141 tctactcttt aaacatacat catgtccaca acttgcaaaa gtttgtacaa taccacttcc
     4201 catagttcca ctacctatta cagctaattt catagttata acctcctgtt attttagtat
     4261 ttatattctg ttaactagat taaaatacta tttaatctag ttaacagcaa atatattttt
     4321 aattagattt ttaccctttt ataaagttag cttctctctt ttcaacgaaa gctgacattc
     4381 cttctttttg gtctttagtt gaaaaacaaa gaccaaataa attagattct atatctattc
     4441 cagtatttat atcagtttga gcaccaagtt gtattgcttc tttagagtat ctaactgcaa
     4501 gctgagcatt tttagctatt atcttagcta atttctcaac ttcttctatt aaaatgtctg
     4561 gctcaacgac tctatttact agccctattt tttcagcttc atcagctttt ataacttgac
     4621 ctgtaaagat taattctttt gcttttgcca ttccaaccaa tcttgtaagt ctttgagttc
     4681 ctccatatcc tggagttatt ccaagagtta cttctggctg accaaattta gctttagcag
     4741 atgcaattct tatatcacat gccattgcaa gttcacatcc tccacctaaa gcaaatccgt
     4801 ttacagcagc tatcactact tttttactat tttctatttc tccaaaagct tttgctccta
     4861 agatactaaa atctttagca gctacagcat ctaaatcttt catgtatgca atatctgctc
     4921 cagctacaaa tgcctttcct tcccctgtca atattacaac atcaatagtt tcatcattat
     4981 taatatctac aaatacttca taaagttctt ctaaagtctt tgaatttatt gcattaaggg
     5041 ctttaggtct attcattttc actgtacata tatttccatc tacttcaaca gctacattct
     5101 cataaacttt aacatcactt gtactcataa taccctcccc aagaaattag ttagctaaaa
     5161 cttcaccttt ttcttttgca acacttaact gactgataag ttctggaagc actttatgaa
     5221 catctccaac tataccaaca tcagcatatt taaatattgg agcttctgga tttttattta
     5281 tagcaactat aaactcagca tcttccatac cagctatatg ttgtattgct ccagatatac
     5341 cacatgctat ataaaggtct ggtcttacag ttttaccagt ttgaccaact tgtcttgctt
     5401 tatctaacca acctgcatct atagtggcac gagaaccaga aacttctcca cctataattt
     5461 cagctaattc ataaagtatg tctaagtttt cttttccacc cattccacgt ccagcagaaa
     5521 ctaatatctt agcatcttct attttaactt gttttttagc ttcttttatt acttgtacaa
     5581 cttgaactaa tttatcagca tcattaaatt ctaccttgaa acggttaatt acagcttctt
     5641 tagtttcatc aggttcattt ttcttcataa cccctggtct aactgtagac atttgaggtc
     5701 tgaaatcttt acaaactatt gttgccatta tatttccacc aaaggcaggt cttgtcatta
     5761 ataataattt tgtatcttca gctactgcaa gacctgtaca gtcagcagta agacctgtat
     5821 gtattctagc agaaactcta ggtgctaaat ctctacctat tgaagttgca ccaaataata
     5881 caactatagg gtcagctgct tttattgctt cataagctgc ttttgtatat ggttcagttg
     5941 tatacactgc taaagcttca tcatctacta ctattacctc atctgcacca tagtgtgcta
     6001 atgtatctat taaaccttct accttactac ctaaaagtaa tgcagaaact tttgtatcat
     6061 aatcttttgc tatttctgta gcctttccta gtaattctaa agaaacagtt tgaattacat
     6121 tttctctttg ttctattact actaaaacgt tacccattct ttatcccccc ttaaattata
     6181 tgatatactt ctcttttaat ttatctatga taattccagc tgatgttttc gcatcttcat
     6241 tgtatattgt accagctggt ttaactgatt ttgtaaatga tttaaataca ctagttggag
     6301 aacctttaag acctaaatta gaagggtcaa cttctatatc ttttacagtc catgtttcta
     6361 ctacatcatt ttcgaaagca tcatatattc ttccaacttt catgtatctt ggtgtgttca
     6421 tatctttaag agttgttata aggcatggca ttttaacttt taagtcatgg caacaatctt
     6481 caaattgtct ttttactagt acatattcac cttcagtttt tatttcttca gcatatgtta
     6541 ttgatggaag atttaaatgt tcagctattt gaggtccaac ttgtgcagta tctccatcta
     6601 tcgcctgtct tccagctatt ataatatcaa aatctatatt ttttaatgct cctgctaatg
     6661 ctgatgaagt tgcccaagta tcagcacctg caaatgctct atctgttaat aatatacctc
     6721 tatctgcacc cattgctaaa gcttctttta aagccatatc tgcttgagga ggtcccattg
     6781 ttataacagt tacatgagca cccatttctt cttttaattt tatagcttct tctaaacctg
     6841 ctttatcatc agggtttatt atacttggta ctccatctct aattaaagta cctgtattag
     6901 gatctagttt aacttctgtt gtatctggaa cttgttttat acaaacgact atattcataa
     6961 atccttcctc catatctatt ttaatatcga cctattgtgt atattttaat atttatctat
     7021 tttaataatt ttcctgaaat aaccattctt tgaacttcac tagttccttc atatatttca
     7081 gttatcttag catctctcat cattctttct actggatagt cacgagtgta tccatatcct
     7141 ccatgaagtt gtacagcttt tgtagtaact tccatagctg tttcagctgc aaataatttt
     7201 gccattgctg cttctactcc ataaggtttt cctaagtctt tatttatagc tgcttgatat
     7261 acaaggtgtc tagccgcttg taccttaact tccatatcag ctaattggaa ttgtgtattt
     7321 tggaattttg ataatggtct accaaattgt actctttctt ttacatattt aacagtttca
     7381 tcaagagcac cttgtgctaa acctaaagct tgtgcagcta taccaattct accaccatca
     7441 agagtagaca ttgctatctt aaatccttga ccttcttttc caagtaaatt ttctttaggt
     7501 attctgcaat cctcaaatat taattcactc gtagctgaac ctcttatacc cattttcttt
     7561 tctttaactc caaagctaaa cccaggagtt cctttttcaa ctataaatgc tgatattcct
     7621 ttgttcccct tagatttatc agtcattgcc attactacat atatgtcacc agctattgca
     7681 tttgttataa atatttttga gccattaagt atgtattcat ccccgtctaa aacagcagtt
     7741 gtttgttggc cagacgcatc tgtaccagca ttaggctcag taagaccaaa tgctcctaat
     7801 ttttctccac ttgctagtgg tcttaagaat ttttgttttt gttcttcatt accatattga
     7861 tatataggcc atgagccaag agatgtatga gctgataata taactcctgt agtaccacaa
     7921 actctagaca attcttcaac tgccattata tatcctacag tgtctccacc ttctccacca
     7981 tattcttttg gatatggtat acccatcatt cctgcttttg ccattttttc cactgtttca
     8041 taaggaaatc tttcttcttc atcaagttct gttgctaaag gtttaacttc attttcagcg
     8101 aagcttacat atagctcttt aagcatctga tattttttag aatttaaatc cataatagcc
     8161 tcctcagtat tttataaata ttttattttc atttattaat taaaatctcg gcaacatcta
     8221 taataaggta tttgaatata cgtcgagaaa tttataacct taatcaaact acgctctata
     8281 tttatatgtg attatactaa ccaattatga cattaattta atcataaatt gttaatttaa
     8341 aatcaactat acttaaaaaa aatctcgctt tttggaattt tgtgaaattt caagttagaa
     8401 taagatttct ttcacactgg ttacagataa aataatttgt ttacgatatt tatattaggg
     8461 tatattaaac attaaatatt gtctgtacat ctgtttttta gccatgtaca gaagattttt
     8521 atcgtataaa aattatttta atagagctaa ataatcgtat ttttatctga ctatttttta
     8581 tttttaaata tcttacatct ataatagtat cactcaaagg ataatttttc aataatttct
     8641 aattattttt ttaacaatat ttattacatt ttttccactt ttttgtcatt tttttattgt
     8701 ttcgaaaaaa aatcagaatt ttttaacttt attttggatt attagtttaa attagatttt
     8761 cctcttagat ttataaatat ttacacaaaa aaactacctc tgtatagatt gtatacagag
     8821 gtagtttttt actaattcct actatagttt actatatttt tttaccaatt cttacaaaca
     8881 tctacccatt tttcacactt agaaaacttc tccatttctt cacatgtaag ttctattgca
     8941 gagttactac tgccacatgc aggaaatact gtatcaaatc ttttcattga atcatctaaa
     9001 tagactttta tagaattttt taaaccaaat ggacagactc caccaatagc atgtccagta
     9061 aactccaaga cttcttctgg tgtcaacatt ttagccttac aattaaattc agccttatac
     9121 ttcttattat caatcttagc atcaccagct gtcacaatta aaattgcatc atctcctatt
     9181 ttaaatgaga gtgtcttagc tattcttgct ggaataaccc cagctgcttc agctgcaagt
     9241 tccacagtag cactagattg ttcaaattct aatatactgt cttccttgcc aaattgttta
     9301 aaatactctc ttacattact tattgacatt aaatacccct cctagtctga acttttataa
     9361 atttcatatt attcttaatt atatctataa ttgatttatt tttcaaatta ttccataata
     9421 ttagcattcc atgtatcatt attttttcca actactagat aaagtccttt tgccattgga
     9481 atattgacaa tatattctcc tttatactct acactcatac cagattcatt aaagtaccta
     9541 taatcaaaat ttagtccttt tggaattaca aaatcaacat aaccttgatt atttggtttt
     9601 attccatatt tcagagttct ttcagttata gaattttttt cattaaataa atctacattt
     9661 acatatgcag aacacttatt cattcccttt ttattttcca cacaattttc aaacttcatt
     9721 ttagcactgt ttaatttata actatattta ctattattgc ttattttaaa tcttacaata
     9781 gtaccaacat ctctacttct cctgctatca aaatcaaaaa caatttcctc taaatgattt
     9841 atttcataat ctttgacact taccatatct ctatatacta atttatttat agctatgaat
     9901 agtgtaaaaa tccccaaaat aaaaaatgaa acttttataa acttcttcat ttttagtctc
     9961 cttatatttt gtgctattgt gctataatat taatcaaaat tttaataatt aaacttattc
    10021 ttttttgaaa tcttaataga aaaatacatt gatattataa aaattattat tgttaaaaat
    10081 gtcatactat ataaatttat atacttagta ttagctatat cagttacata atttgaaaga
    10141 tatactcctc caactttact ataattaaaa tgctttactt gatatacaat catactaatc
    10201 atcttcaaaa gacttccata tacaaagtaa attaaaatac ttactataaa accacaaact
    10261 ttcatttttc catatccaaa cttaaaaatt aaaggcagta aaatcgtcaa aattgctaaa
    10321 aatatattta cagaaaaaaa tacatcattt aaaaccatat ttctaaagaa cactagtgat
    10381 gtcaatttac ttagtaagga cacaattatt gttacagaaa ttattaatcc tacacctaat
    10441 aaatattttg agtaaactat atcttctata ctaactggca tactccttat aaaaatcctt
    10501 gaatcatttt cataatcaca ctcaaacgta tttataaata tcaaataact aacaacaata
    10561 cttactgtat aatatgaaat tgaattaagg aaaaaataaa acacaaaaaa tagcaatatg
    10621 tactttaaat tacggatacc atctgaacta atccctatta ttaaatcttt tcttattaaa
    10681 ttgaacatca tctcacctca tttacataat aatacattag ttcttctata gtaggatttt
    10741 tgaccttatg cttatcaatt tctatcaata ggctttttac tttcaagcta tctccaactt
    10801 taacaagtgc ctcattataa tatttatact ctttcacacc aactatgctt tctttaattg
    10861 cttctaactc tgctttattt gccctaatta tcttatattc ttgttctatt aattctttct
    10921 taacagtaaa aatctgattt cctctattta taaaaacaag ataatcagca attttaatta
    10981 aattatcagt attatgagta gaaattatta cactactact tccatttttc acaaaatctt
    11041 gtaaatactg cattatttca gttcttacaa ttgggtctat accagcagtt ggttcatcca
    11101 aaatcaataa cttagcattg tgactaagtg cgcttgataa cataagcttt tcagattgac
    11161 ctttagataa attttcaata tgcatatttt cttctatatc aaacttatta aggtattcat
    11221 caaacctttc tctatcaaaa ttcttataaa ataaagaaac tatttttcta aattccttaa
    11281 cctttaaatg cggataaaat tgtaaagaat catatacaaa accaatattg tttttaaagc
    11341 ttattggaga attttcaata gtttcattac aaagttcaat ttcaccttca tctgcatcaa
    11401 taatccccat tataattttt attaaagtcg tttttcccga accatttggt cctataatcc
    11461 caacaatatc cccttccttt aactctaagt ttatattatt taacttgaat tttcctaatt
    11521 tttttgtgat attccttatg ctaaacacat cttcacctct tatgcatttt tttataccca
    11581 cctatacata ttttaacaca ttcattacaa aggtcattta taaatttata aattaagaat
    11641 tataaaacta tgttataata caaagtatga agaggtgaat atatgaaaag aattataact
    11701 ataaatacaa accaattaaa taagtccctt gagactaaag aactcttaac tagaaaactt
    11761 atcaatgctg gctttgaagt gtattcagat atttatccag acacagagct tattatatca
    11821 attggtggtg atggctcttt tttaagaaca gttagagatt tcgattttcc tgagattccc
    11881 ataatgggaa taaatacagg tcatctgggg ttttttcctg atattttacc agataagatt
    11941 gatagcttta ttgaagctta tacaaaaaaa gattatatta ttcaagaaat gtcacttctt
    12001 aatgcggaag tatatactac tactagtggt tctaatatgc ttgctgtaaa tgaggttgtt
    12061 ataagaggag acaagtctag gactatacac ctaaatttaa gccttgataa taaacatata
    12121 caaaatttta gtggagatgg tatgattatt tctacttcca caggttcaac agcctataac
    12181 tattctgctg gaggtagtat tgttgatata aatcttgaac ttatgcagat tacaccttta
    12241 catccgataa ataccaatgc atataggtgc tttacatcaa gtataatatg ttcaaatgaa
    12301 tctgttataa agattgcacc agaatataga tttgaagatt ctgtattgat tgtcgttgat
    12361 ggagttgagc acagatttag acaaatcgaa aatataaaag ttagtatatc tgatgcaaaa
    12421 ataaaattac ttagaatgtc aaactatgag ttttggcata gagtatctga aaaattttta
    12481 tagactgtac ttaggaggat ttttaatatg aaaataggaa taatgagtga tactcatggt
    12541 agcttacttt actttgaaaa agcgttaaat gtattatctg attgtgatgt tttattacat
    12601 ggtggagatg tcttatatca tggacctaga aatgatattc cagaaggata taatcctaaa
    12661 aaatttattg agacattaaa taaattagaa aacattgtga ttgtaaaagg gaactgtgat
    12721 gcagatgttg accagatggt tatagaacat cctattcaga gtccttatgt catgagccaa
    12781 tttggtgaaa ttagaattat attgaatcat ggttatatag aatcagaaga agaaattatt
    12841 gacaaagcaa agaaaatggg tggtgacata ttagttttag gacatactca tgtaaaaaaa
    12901 ctatacatgg atgataattt aatagtaata aatccaggaa gtacttctat cccaaaagat
    12961 ggaagccatt ctgtcgcaat tatagatatc ataaaaactg atgatgagga tgagcttgaa
    13021 cttgatataa acttaattga catcaatact ggaaatataa taaatatcaa tgattagtaa
    13081 atttttaata aaaataaaat aagctttaat ttattaaact cataataaaa acaagtaaac
    13141 cattttattt ctatctcaat tagaagttta agtacaatac cttaaacttc taattaaata
    13201 gaatctggtt tacttgtttt attatatgct atattgaaat ataacttagt tt
//
LOCUS       DAEOSP010000110        13184 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.110, whole genome shotgun sequence.
ACCESSION   DAEOSP010000110 DAEOSP010000000
VERSION     DAEOSP010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13184
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.110"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(6..2006)
                     /locus_tag="KRH54_002360"
     CDS             complement(6..2006)
                     /locus_tag="KRH54_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8580017.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            complement(2037..3755)
                     /locus_tag="KRH54_002361"
     CDS             complement(2037..3755)
                     /locus_tag="KRH54_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBG8580018.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGSYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIIDSIRVAIANKKWNDDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            complement(3981..5219)
                     /gene="fabF"
                     /locus_tag="KRH54_002362"
     CDS             complement(3981..5219)
                     /gene="fabF"
                     /locus_tag="KRH54_002362"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBG8580019.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            complement(5248..5472)
                     /gene="acpP"
                     /locus_tag="KRH54_002363"
     CDS             complement(5248..5472)
                     /gene="acpP"
                     /locus_tag="KRH54_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBG8580020.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            complement(5526..6275)
                     /gene="fabG"
                     /locus_tag="KRH54_002364"
     CDS             complement(5526..6275)
                     /gene="fabG"
                     /locus_tag="KRH54_002364"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBG8580021.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            complement(6275..7225)
                     /gene="fabD"
                     /locus_tag="KRH54_002365"
     CDS             complement(6275..7225)
                     /gene="fabD"
                     /locus_tag="KRH54_002365"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBG8580022.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNTVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            complement(7241..8170)
                     /gene="fabK"
                     /locus_tag="KRH54_002366"
     CDS             complement(7241..8170)
                     /gene="fabK"
                     /locus_tag="KRH54_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBG8580023.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            complement(8279..9265)
                     /locus_tag="KRH54_002367"
     CDS             complement(8279..9265)
                     /locus_tag="KRH54_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBG8580024.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            complement(9291..10313)
                     /gene="plsX"
                     /locus_tag="KRH54_002368"
     CDS             complement(9291..10313)
                     /gene="plsX"
                     /locus_tag="KRH54_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBG8580025.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            complement(10324..10881)
                     /gene="fapR"
                     /locus_tag="KRH54_002369"
     CDS             complement(10324..10881)
                     /gene="fapR"
                     /locus_tag="KRH54_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBG8580026.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            complement(11088..11264)
                     /gene="rpmF"
                     /locus_tag="KRH54_002370"
     CDS             complement(11088..11264)
                     /gene="rpmF"
                     /locus_tag="KRH54_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBG8580027.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            complement(11289..11813)
                     /locus_tag="KRH54_002371"
     CDS             complement(11289..11813)
                     /locus_tag="KRH54_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBG8580028.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            complement(11906..13102)
                     /locus_tag="KRH54_002372"
     CDS             complement(11906..13102)
                     /locus_tag="KRH54_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBG8580029.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
BASE COUNT         3867 a         2602 c         1376 g         5339 t
ORIGIN      
        1 ttagcttaat ttatattatt ataaagtttt atcccctctg aggttatttg acttcctcct
       61 ctaccagatt tagatgatat gtatccacat tcacttaaat aagatagcac ctttctaact
      121 ttgctttcag aaatctcaat accttcctgt ataagctttt cgattataaa ccctcttcca
      181 attccagagg atgactctct tctttcatat agaatcctca gtatttcctc aaattctttt
      241 cttgtataac caaatccatc ttcataattg ttagcacatt ctacccttat atatggtggt
      301 aagtcatctc taaaaaccat actatcacac attagactta cataagaagc aacattttgt
      361 aactctctta tatttcctgg ccaattatat ttttctaaaa tagttataac ttctggcgaa
      421 agtcttatat tgtttttcaa aaagaaattc ataagtgtta ttatatcttc ttttctattc
      481 ctcaatgatg gtacatctat tggcaataca tttagtctat aatacaggtc ctctctaaat
      541 tttccttctc taatcatctt tagtaagttt ttgtttgttg ctgctatgac cctaacatct
      601 atgtttataa cattatggga acctataggc ataacctgct tttcttgtaa tacccttagt
      661 aactttgtct gtagcaatgg aggcatatct ccaatttcat ccagaaatat agttccatta
      721 ttagcaagtt caaatagacc cttttttcct tctcttaaag ctcctgtaaa agagcctttt
      781 tcatatccaa acagttgact ttctaataga ctatctggaa ctgctgcaca attaactgct
      841 ataaacggtt gattacttct attggaagca ttatgaattg attgtgataa taattcttta
      901 ccagtaccac tttctcctat tattaataca gaaaggtctg atttagatat ttttttagcc
      961 aattctacac actttaccat atttttattt ttagttttta tatctttaaa tgtatatctt
     1021 gctatctgac cttgttctct taatttatta gtaagatttt gttctaattt ttttatatac
     1081 gttatttctt gtaagttata atatatccca gctttattcc ccatagaaaa aatacttttt
     1141 ttatttacat ttatatattt tttatttgta aataccactt catcaattat ttctttgtct
     1201 tcaagtaaaa tatttaaatc ttcagaaatg atatcttcta tgtttttgcc ttgtggattt
     1261 tcatttatac ctaaaatttt taaagcttct ttattaaatg tgtttattat accatctgct
     1321 gaagtaaata ttattccatc ttttgataga ttcaatataa catcttgttc ttctattttt
     1381 aaaaacagtt gcctataatt atctttaact ccattatcta aacttattaa actctcagaa
     1441 tatgccatta aatttttacg tatttcttta acatctattc taagaccatt tattatttct
     1501 ataaaggtag atatatcaat atatctattt ccaaggtcga ttactttatt tatataactt
     1561 ggtacttttc cctttactcc aggcgttata gctatgtcta tatttttata aatttttcct
     1621 tcaatatatg gagtaaactt taaatttttt actcctattt tgtaaaataa gcttatcgtt
     1681 tctaaggtag tttcttttgt atcatttaca accaatactt cagttccatt tggtattgaa
     1741 aataatccat atatttcatt ttctttaagt gttctcctta tcactataat cttactatct
     1801 tgtactaaat gctttctcat actgtttaat ctatcatcat tcattactag tacaaaatca
     1861 ccttgtattt tatcattctc ttttaaaact tccagataat agtggtttat atcaatataa
     1921 ttacctaaaa ccaaacgtat attttcttct aagaaagatg atagtttagt tattttatca
     1981 gttactatag ctattgattg tttcatattt atacctctta tttatatagt atataattac
     2041 ttatatacta ctttatttct accaagttgt tttgctttat ataaattttt atcagcttct
     2101 tctagtacat ctttgctatc tgaaatatga gctacacctg cacttatagt cacagtcaca
     2161 tcatcattcc attttttatt tgcaattgca acccttatac tgtcaattat atctgctact
     2221 tcctcaccag atttatttct tattaataca gaaaattctt ctcctccgta cctaaagact
     2281 ttatcatctt gttctaacat aacacaaatg gtatttgtta ttccctttat aacagtgtca
     2341 ccaaatttat gtccataatt atcatttatg agcttgaaat tatcaatatc tatcatcaca
     2401 agtgcaaaat tatcatcttt ctctctgtac ttttcataca taatatcaaa actttttcta
     2461 ttataaacat ttgtaagacc atcatgtaaa ttttgtgctt taaaatactt caatcggttg
     2521 tataaaaaag tagaccctaa tatagcaatt aaaattactg taaataatat tttataatat
     2581 tttatttctt ttttagtttt ttcaaatagc ttactttcgt ttgatacttt atcaattata
     2641 tagaaactag tctctgaatc tcttatactt ttgttctctt tatattcatt tattaacatc
     2701 ttttgatact catttaaaga ttctatattt ccttgttttt catatatatt taatatctta
     2761 tttaaatttt ttattttctc ctgggttaac tttcttttag tagatatctc taaacttttt
     2821 ttataattat ctattgcaga attataattt tcgcttttat attctaagtt tcccattgcc
     2881 atataatagt acatatcttt atctaatata taaactgttc tatctgattt tataagataa
     2941 tcagcttttt ctaaaaaagt ctttgccttt tgtatattat ttttgcccac atacgcattt
     3001 gactgcatta tagaggccat tatatcaaaa tcattgtaat tatccgtatt tacaaacttt
     3061 ttataatctt ttatcctgct acttatttca atagctttat catattctcc ctcttctaca
     3121 tatatttctg ctagatttat aatagcatat aatctaacca ttccattttt ttcaccttca
     3181 tcaatatcta ttttaaaaga atcctttatt aatttctctc ctgtttcata gctacctatt
     3241 tttaaaaata tgctcgccaa atcaacatct agtcttgctt tttcataatt attccccaat
     3301 ttctcagcca actttataga ctttacagtt aaatctactg ctttagaata attattatcc
     3361 attatataca ctgatgctaa tcttttttct atattaaatc tacattcatc agataatttc
     3421 acatctgatt ctctcatctt ttctaaagaa tcaatgacta tcttcgttcc atctggaaca
     3481 tatgaaatag tctttaacat atcccaaact tcattatata ctttattgta ttttttttct
     3541 ggaatagatt cgaaagaagt cttaaccact ttttttgctt catcatactc cttatctgcc
     3601 acaaggcttt cagacatata ttttccctta tttataattt ctctttcttt caatccattt
     3661 tggtcatact ttataaacaa aataatacta ataagtatta tacatataaa aattatcttt
     3721 ttgggtaacc ttttctttgg tagtctaaaa ttcaaaattt tagtcctcct tttgtatatt
     3781 gtagaatgaa aacttgcata tataaataac ctcttaataa ttaataatac aatatatcaa
     3841 aatgatttac aacatatgct agacttaaat cttaataaaa aagtctccta aaataattta
     3901 ggagacttta aatttaagct aatataaatt tatacagtta aaacaagtgc ttgtttttta
     3961 gttttatatt taatatttat ttatacatat ttttttagta ctattgtagc attatgccca
     4021 ccaaatccta aagaatttga tagtgcatat ctaatatttt tttctttacc ttgatttgga
     4081 acataatcta aatccaaatc ttcaccaact tcttgtatgt ttatagttgg aggcacaaaa
     4141 ctatcttcta tactcatagc acaaacaaca gcctctatag cacctgatgc tcctaataag
     4201 tgaccagtca tagattttgt tgatgacaca cataagtcat atgccttttc tccaaataca
     4261 gtttttatag ccatagtttc atataaatca ttgtaataag ttgaagttcc atgagcattt
     4321 atataatcta cttcttctaa tggaacattt ccatctttca aagccatatt catagaccta
     4381 gctgctccct ctccattttc agctggagtt gtcatatggt atgcatcagc agttaaacca
     4441 tatccaacta cttctgcata tattttagcg cctcttgcta aagcatgttc taagtcttct
     4501 aagataacaa tacctgctcc ttctcccatt acaaaaccat ctctgtcttt atcaaatggt
     4561 cttgatgcag ttttagggtc atcatttcta gttgacatag ccttcatgtt acagaatcca
     4621 gcaaaagcaa gacctgttac agcagcttca gagccccctg ctaccatgat gtctgcatca
     4681 cctctttgaa ttaccttaaa agcatcacct attgaatgag ttcctgatgc acaagctgta
     4741 catacattag tgttaggccc ttttgctcct aaaaatattg aaacttgacc agctgccata
     4801 tttcctatca tcataggtac aaaaagtgaa cttactcttt tatttccttt ttccaaaaga
     4861 attttatgtt gagtttctat agcttcaact ccacctattc cagaacctat tataacacca
     4921 aaacgttctc tatcaacttt atctaaatca agatttgcat cttcaactgc taatttagca
     4981 gatactattg caaattgagc aaatttatct aatctcttca attctttttt acttatatat
     5041 tcttctggat gaaaattctt aacttctcct gctatctgag tttgatatgt gctagcatca
     5101 aaattagtta ttttatctat accagatact ccagatttta tattactcca gaactcttct
     5161 tttcctgttc ccaacggcgt aacacatcca agtcctgtta ttactactct tttattcatc
     5221 ggatacctcc acttttttaa agctcgacta tttattagct tctatatact taactatatc
     5281 tcctatagat ttgaagtttt ctgcttctgt atctggtatt tctataccaa attcatcttc
     5341 aagtgccatt attatttcta ctgcatctaa tgaatctgct tctaaatcct tcattaatga
     5401 tgtttccata gttatttctt tatcttctac tcctaattgc tctattatta tttctacaac
     5461 tttattaaac attgtgtacc tcctaaaatt ttttatttta atagatttct ctacttttta
     5521 aattattatt gcattaccat tccaccatct acatttatta cttgtcctgt tatataactt
     5581 gacatgtcac taactaaaaa tgctacggca ttggctacat cttctggttt accataagat
     5641 ttctttggta tgtttgctag tgttgattct tttacatcat cacttagtac tttagtcata
     5701 tctgtttcta taaatccagg tgctactgca tttatattta tgtttcttga tgctaactct
     5761 cttgcagaag cttttgtaaa tccaattaca cctgcttttg atgcacaata attagtttgc
     5821 cctgcattac ccataattcc aactactgaa gtcatattta ttattcttcc atacttcttt
     5881 ttcatcatag gtttagtaac tgcttttgta caattaaaga caccttttaa gtttatatct
     5941 ataactctat caaaatcttc ttctttcatt ctcataagca gaccatcttt agttatgcct
     6001 gcattattaa ccaatatatc aatttttcca aattcgctta caacagaatc tatcatttga
     6061 ttcacttcat caaatttaga tacatcacat tttatagagg tacactttac ccccatactt
     6121 tctatttcat ttttagtttt tagtgcttca tcttctttag aagtataatt gattactaca
     6181 tcagctccaa aagatgctag tttttttgct atttcttttc ctatacccct agagccacca
     6241 gtaacaactg ctacttgtcc tgtaagattt atcattatat ctctccattt ctataatttt
     6301 gtacaaattc attaagagaa tctataccat ctatattata aatattgaca tttgcttttt
     6361 tattggaagc tatcttctta ataaatgctt ttaaagtttt tccaggtccc atttctataa
     6421 aagtatcata tccatcattt aaaagtcttt ctatagaatc ttcccaaaga acagatgaac
     6481 ttacttgttt tataagcagg tctttacctt cgtttttata gtaatctgca tttacatttg
     6541 ccaccacatc aactttaggt ttagttatgt taactttttc taattcactg gccaatttta
     6601 caccagcttc ttttaacatt gatgagtgaa aaggtccact tacatttaat ttaactgccc
     6661 tttttgctcc tgcttctttg cattttacca ttgcttcttc tatcttttca ttttcaccag
     6721 atataactat ttgtccagga gaattgtaat ttgctacttc tacaatacct ccttgtatat
     6781 ctttaaccac attttcaagt acatttctat ctaaacctaa aattgctgcc atagctccaa
     6841 cacctgctgg aactgtttcc tgcataaact tacctctttt tttaactaaa catacagcat
     6901 ctttaaagtc aattgcatct gctgcaacca acgcactata ctctcctaag gataatccta
     6961 agcatgcatt atattttaaa tctatttgag cttgtaccgc ttttaatact gctacactat
     7021 gtgtaaccat agcaggttga gtgttttcag ttttagaaag ttcttcttca tttccattaa
     7081 acataaggtt ttttaaatcc atatttaagg catcactagc ctcatctatt acacttttag
     7141 caacagtatt attttcatat aaatccttgg ccatacctac atattgagct ccttgaccag
     7201 ggaatactag tgcaactttt cccatcttgc tcctcctgat ttacagttct attgctggca
     7261 taatctctct tgcttgattt accatagcct ctataatttc cttacaaggt gttatttctt
     7321 ttaccatagc agcactttga cctgccataa atgaaccttt ttctatgtct ccatctactg
     7381 ttgcaaatct taaagctcct gtaccttttt tatccagttc ttcaggagta gctccatttt
     7441 gttccatctt taaaaattct tttgatagtt tattttttaa tgttcttact ggatgaccag
     7501 tacttcttcc tgttacaatt gcatctctat cttttgcttt tagtactaag tttttatagt
     7561 ttgaatggac agaacactct tcactacaaa taaatctagt tcctacttga actgcactgg
     7621 cacctaatgc aaatgatgct gcaattcctc taccatctac aattcctcca gcagctatta
     7681 caggtatgtt tacagcatca gcaacttgtg gaactaagac catagtagta agttctccta
     7741 tatgtcctcc accttcagta ccttctgcta ttactgctgt agctcctagc ttttccattc
     7801 tttgtgccaa agctattgta ggtactacag gaataacctt tgttccagct tcctttaatc
     7861 tatccatata ctttgcagga tttccagcac cagtagtaat aacttgaact ttttcttcta
     7921 taatcaaatc aattatatca tcaacaaatg gcgacataag cattacattt actccaaaag
     7981 gtttatctgt taatttttta cactcaacaa tttctttctt tatagcttct tttggtgcgt
     8041 ttcctgctgc tattatgcca agtcctcctg cattagatac agcacttgct aatgatgcag
     8101 tagctaccca tgccattccc ccttggataa ctgggtattt tatatttaat attttgcaaa
     8161 ttttattcat acaattctcc ttcacttgaa atcataaaag taaacttttt attattaatc
     8221 aactttattt tgtgtataag tttaattttg ttaattaaat cattttagat attttatact
     8281 ataaagtcca cttgacaact gaagcccccc aagttagacc tccaccaaag ccaactaaaa
     8341 ctacattatc acccttttta attttacctt ctctataagc ttcatctaat gctactggta
     8401 tagatgcagc agacatattt ccatatttgt ctatatttac ataaactttt ttcatatcaa
     8461 gctctaatct cttagaagaa gcttgtatga ttcttatatt agcttgatgt ggcactaagt
     8521 agtctatatc agttgtattt agatttgctt tttcaagagc tcttaaagaa gtttctgcca
     8581 tctttctaac tgcaaattta aacacatcac tacctgccat ttctattgta tgtagattag
     8641 cttctacagt ctctttactt gccttaagtc ttgaacctcc agcaggtact tttaaacaat
     8701 cttttccttt accatcagag cccatatgtg tagacagtat tccaccctca tttacagggc
     8761 caataacagc tgctccagct ccatctccaa acaatactgc tgttgttcta tcatcataat
     8821 ttaaaaactt agaacaagtt tctgctccta ttacaagcac atttttatag caacctgttt
     8881 ccacaaattg ctttgcaata gttactccat atacaaatcc agaacaagct gctgatatat
     8941 caaatgcagt agcattcact gctcctattg catcttgtac tatacatgca gttgaaggca
     9001 aagacatatc aggagtaatt gtagctaata taatcagctc tatatcctct acgtttaact
     9061 ttgcacattc tatagctttt aaggcagctt tagatgctaa gtcagaagtt gcttcagact
     9121 ctttggcaaa tcttctttcc ttaataccag ttctagtact tatccactca tctgatgtat
     9181 ccattatttt ttcgaagtga aaattatcat atgattgctc aggtagataa cttcctactc
     9241 ctaatattcc agcctttgta ttcattatat attaaacctc caaaacgagt ttattcatct
     9301 tcgttatttt tattttcttc attgtatttg cttataaatt ggttaatatc ttcaacgact
     9361 tttccctttg aaaattttat tccttgattt atagcatttt ttatagcttt agcatcagaa
     9421 ctaccatgag cttttataac tcctccattt agacctaaaa gtggagctcc cccatactca
     9481 gaataatcta taaaactttt taattttctt aaatcatcct ttataagtaa tgctcctatt
     9541 ttactttttg tactggccat aaaagtttcc tttataagac tcataactga aagtgctaca
     9601 ccttcagcag atttaagtaa tatattacct gtaaatccat cacatatgat tatatctgtg
     9661 tatgcattta taacttctct agcttctaca ttccctataa aatttaaatc taatttttta
     9721 atttcttcat atgacctttt gactaaatca ttgcctttgc cttcttctag tccaacattt
     9781 gctaatgcta ccttaggatt ttcaagacct aaaacttttc ttgaataaat attactcata
     9841 gctgcaaatt caactagatt cttaggttta caatctgcat ttgcgccaca gtctgctata
     9901 agtgtcattc ctcgctttac atttggtatt gctgaacata agcaaggtct atcgattcct
     9961 tttattctac caacaacaaa taatccacca gcaagtaagg ctcctgtatt tccagctgat
    10021 attatagcat ctgcctttcc ttcttttaca agatttaatg ctactaccat agatgaatct
    10081 tttttacttc ttatagcctt tactggttta tcttcgtttt ctataatctc agtagtatga
    10141 actatctcta atttatttct atcaaattca taatttgaaa attccttttc aagtaaatct
    10201 ttatctcctg tgattattaa atcaacctga tattctttta tagcatttac tgcgccttct
    10261 acattagatt taggtgcatt atctccaccc attccatcaa ttactatttt cataatttcc
    10321 ctcctaatct aattcttcaa agataaactt tcctctaaat atttgctctt gggctttatt
    10381 gttgataaca acttttacat aatgcttttt atctatattt ctatacactt ctgcttttgc
    10441 tataagccta tcttgttgtt ctatcaactt aaaacttttt acatttgcaa ctctcataat
    10501 aaccttaggt gcatctatta tagccattgc caatgaatct gctagagaaa aaatatacgt
    10561 atcttttaaa gtattagtct tagagtaaat catgtcctgt gtaacttcta acatagatat
    10621 acctaatcgc cctacagata aatcaatgat ttctcctgta atttccttta ctcctaaagt
    10681 ctttactttt gaactctgag tttctgctat tgatttgact ctttctctta gctctggtat
    10741 gtttagactc attctatcta atcttatggt ctgtatactt acatcaaaca gacttgataa
    10801 ttcttcatca gtataaaaag gatctgtctt tagcatatct ataagctctt tttgtctttg
    10861 ggctttactt ttctttttca tgttccctcc aaattttata gctgtaatta gtaccagcta
    10921 ctaatagtag tatatagtaa cacacaaaaa ttttcaatac ttatattatt atttttctat
    10981 tttttaatat gcataaaatc cacttatttt ctctagtaaa ttatcttatt tttttatatg
    11041 tacaaaaaaa acatgtagag tatactctac atgcttttta gaagctatta cttagacaca
    11101 acttctttac ctttataata tccacagtct ggacacactc tatgtggtaa ttttggctca
    11161 tgacattgtg ggcaacttac aaatcctgtt gcttccatct ttgagttagc tgctcttctc
    11221 atttttgtgt ttgatttaga cgttttacgc tttggtactg ccatttacgc cacctcctta
    11281 gtcaaaattt aattaaataa atcttttaat ttagcaaaac gaggatctat gtattcctca
    11341 tcgtttgtaa tttcactgca agaacactct tcttcattta agtttgcccc gcatacttga
    11401 caaagacctt tacagttttc actacaaaga actttatgag gtacattaaa atctaatgtc
    11461 tgttctatta tatcaataag gtcgatttct tcaccatcga atataaaagg atcaaattcc
    11521 tcaaactcat cttcatcata atcttcttct tttactaaaa aaccttgtat agaatactct
    11581 agtggtattt ctacttcttt aagacatctt gaacaattgt caacaattgt aaaattgaca
    11641 tctatatcta gatacaaacc tttattagtc tttgacactt tcccttttaa atttacaggt
    11701 gatactaatt tgtaaatctc atcacaataa ctaatagtat caattttttc acaaaaattc
    11761 aagtctattt tgtcagtttc ttttctattt attttttcta tgctaacttt cattaagttc
    11821 acctcaacat atcaccaaat ttattataca aatctagcat ttgtttgtca agtattttta
    11881 cttgataggc ataggtttaa tttaattatt ttaccaatct taatgtatct ctagctatca
    11941 ttaattcttc attagttggt attaaaagca tttttacttt agaatcagca gtagatatta
    12001 ctgtttcttt tcctctaaca tcattagctt ctttatctaa tttcattcct aaaaactcca
    12061 tgttagaagc aatatctgct cttatagcct ttccattttc accaacgcct gcagtaaata
    12121 ctacaacatc caatccattc atttcagcag cataagcgcc tatatatttt tgtacttttt
    12181 taacatacgc ttctaatgta gcttgagctc tttcgtctcc atttttagct gcatcttcta
    12241 tatctctgaa gtcactagaa attccagtca ttccatatac tccagattct ttattcatta
    12301 atttatccaa tccatcagca tctaaacctt cttttctcat taagaatggt aatatagctg
    12361 ggtctatatc tccacatcta gttcccatta ttaatccttc taatggagtg aatcccatag
    12421 atgtatctac acattttcca ccatcaacag cagctataga agcaccattt cctaagtgac
    12481 aagttactat ttttaaatct tttatatctt ttcctaatat ctctgctgct ctttgagata
    12541 cataattatg agaagttccg tggaatccat atcttcttac cccatattta gtatatagct
    12601 catgaggtaa accatataag tatgaagatt taggcattgt ttgatggaaa gcagtatcaa
    12661 atacacctac cattggtaca tctggaagta tagctttaca agcatctatt cccattatgt
    12721 ttgctggatt atgaagtgga gctaaatcta tacattcttt taaagcttct tctacttctg
    12781 gagttattac tactgaagtt gcaaattttt ctccagcatg tacaacccta tgtcctactg
    12841 catctatttc tttcatttct ttaactgcac caaattctgg atgagcaaca gcttctaaaa
    12901 ctaatgctat tgcatctttg tggtccttca ttggttgttc aacaacgtat ttatcatctc
    12961 tacctgcttt ttcatgtttt aatatagaac cttctattcc tattctttca accaatccta
    13021 tacataaaac ttcttcatta ttcatatcta ttaattgata ttttaatgaa gaactcccac
    13081 agtttaatac taatattttc attattattt cctccttaga tttttttata aataatatgt
    13141 aataaatttt gatttgtaat aaatgtctaa ataaaactct atct
//
LOCUS       DAEOSP010000111        13124 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.111, whole genome shotgun sequence.
ACCESSION   DAEOSP010000111 DAEOSP010000000
VERSION     DAEOSP010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.111"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(73..282)
                     /locus_tag="KRH54_002373"
                     /pseudo
     CDS             complement(73..282)
                     /locus_tag="KRH54_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
     gene            complement(384..932)
                     /locus_tag="KRH54_002374"
                     /pseudo
     CDS             complement(384..932)
                     /locus_tag="KRH54_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1291..3201)
                     /gene="phnW"
                     /locus_tag="KRH54_002375"
     CDS             complement(1291..3201)
                     /gene="phnW"
                     /locus_tag="KRH54_002375"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBG8580030.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIQFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(3480..4265)
                     /locus_tag="KRH54_002376"
     CDS             complement(3480..4265)
                     /locus_tag="KRH54_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG8580031.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(4482..4712)
                     /gene="dltC"
                     /locus_tag="KRH54_002377"
     CDS             complement(4482..4712)
                     /gene="dltC"
                     /locus_tag="KRH54_002377"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBG8580032.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(4731..5879)
                     /gene="dltB"
                     /locus_tag="KRH54_002378"
     CDS             complement(4731..5879)
                     /gene="dltB"
                     /locus_tag="KRH54_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBG8580033.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWCYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(5879..7393)
                     /locus_tag="KRH54_002379"
     CDS             complement(5879..7393)
                     /locus_tag="KRH54_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBG8580034.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNDIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYRELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(7514..8704)
                     /gene="dltD"
                     /locus_tag="KRH54_002380"
     CDS             complement(7514..8704)
                     /gene="dltD"
                     /locus_tag="KRH54_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBG8580035.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(8992..10011)
                     /locus_tag="KRH54_002381"
     CDS             complement(8992..10011)
                     /locus_tag="KRH54_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG8580036.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(10187..10726)
                     /locus_tag="KRH54_002382"
     CDS             complement(10187..10726)
                     /locus_tag="KRH54_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBG8580037.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(10827..11507)
                     /locus_tag="KRH54_002383"
     CDS             complement(10827..11507)
                     /locus_tag="KRH54_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBG8580038.1"
                     /translation="MQNVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(11536..12825)
                     /locus_tag="KRH54_002384"
     CDS             complement(11536..12825)
                     /locus_tag="KRH54_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBG8580039.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         4234 a         2353 c         1256 g         5281 t
ORIGIN      
        1 gtttccctat atgctgacta ttttagctac taactcatga ctaaagtcac aagtgtgcat
       61 agctattttt taatcaagag aagtctcttc tttcatagac aatattaact ctattctttc
      121 tagtatttta tctcttggaa agaaagtctc ttgacctgta aaagctgatt tttttataaa
      181 ccattcctct ggtattatgt tttttctttt ccatctatat aattgtccat aagatatatt
      241 agttttgctc aataaatctt tcttagatat taattcttcc atagctcttc tagcccacct
      301 ttatactgtt ttttacagta gaattatctg aaactgataa attatcactg tattcaatat
      361 ttccaacatc ataattatct actagagata tgcttttttt ctagcatacg aaattttttg
      421 caataatctc catgcccctt ttttatattt atactacttg ctccaacttc attaaaatta
      481 gaagttccat ttaaactaat attaacttct tcacaattca aaaatccatt acactccaag
      541 attccactta aattgatatt ttcacaatct atattctcat ttgcttttaa atatccttct
      601 ccatgaataa tatccgattt tatacttcct tctacattaa taattccact agtatcaatt
      661 tcaacagaat taatatttcc tgtacatcta agaagtcccg atatctcaac ttttgatgcc
      721 agtatgtttc cttctacttt acttaaacca gatgtaaaaa attcattaca ttctgttgtc
      781 ccaaaaactt ttgctgaacc agaagaatca aatttactac attttacact tccttctatt
      841 ttgcctgttc ctgatatatt aacattatca aaaattccac ctttacattt tccaacacca
      901 tcaaatctta gtgaacttaa ttcttcattc ataatgatat cctttcttta ccgtatttta
      961 tctttaaacc taatataaca aaacattgtt acgttgtcaa gtatgataat aatacttaat
     1021 aaagcaacta tcatttattt tctataaata ggcataaaaa taatagggaa atgctttatt
     1081 taaactataa atctaatttc ttagattgtg gtttaaataa aatatatccc tattactcaa
     1141 tgtttatata ttcattgtat aaacttctaa aatataagtc tttaaattct actgtcaata
     1201 ctttttatac actgtttgtg ctatcatctc acacacctaa aggagtaggc tttgcgcact
     1261 attctgtaac ctacaagaca ctatcttctt ctatctattg ataaattttt ctattacatc
     1321 agctagtctc tccatatcag ttggatagac ttcacctatg ctacctattc taaatgtatc
     1381 tatatcagtc aatttacctg gatagataac aaatccatta tcttttaaat aagtataaaa
     1441 ttccataaat tcaaactttg catttttagg atataagaaa gttgttatta caggtgattg
     1501 agcattatca ttcaccaaag tatcaaagcc taaagatttt aatcttgaag ctattgtaaa
     1561 ctgattttct ttatatctag catatctttt ttcaacagaa ccttcttctt ccaattctaa
     1621 taaagcttga taaaaagctc ttacaacatg tgtaggtgaa gtaaatctcc atttaccatt
     1681 atttttctcc atagtctccc attgcgcata cacatcaaga gataatgatt tagctatacc
     1741 tttacattta gataatttct ctttattagc tattataaaa ccaaatccag gtactccttg
     1801 tatacatttg ttagaactac taactaaaaa atctatatta aaatcttcaa catcaatctc
     1861 aattccccca aaactactca tagcatcaac tatataaatt ttgttatatt cttttgcaag
     1921 tttccctacc tcttgtatag gatttaatct acctgtagta gtttcacagt gaaccattga
     1981 tatatgtgtt atatccttgt tttcttttaa taaattttct actacattta aatccactgc
     2041 ttctatatct ttaaatgtgc agtcaacaaa ttgtatgtta agataattac atatatcttt
     2101 cattctcttt ccatatgctc catttgcaat tacaagaagt ttaccatctt ttgaaatagt
     2161 acttcctatt atagcttcta ctgaaaaagt cccacttcct tgcattaata cacttgtgta
     2221 tttatctgta ttttgggttg ctagagatac taatcttctt ctaacatctt gtactaggtt
     2281 attatattca acatcccaag tacaccaatc ttttaacata gatgctctta ctgatttagt
     2341 agtagatagt ggtcctggtg taagtaatat gtaatcattt tctggtacaa aatctgattt
     2401 aatagtttca ttctctattt ttattagtat atcttctaac tcagccatag tttctattac
     2461 aaaatgtgca cctgcctctt taaatttctt tgaaactata ctcatcttag cttttaattc
     2521 ttccttatcc atgttctcta cttcttcttg tgtaagccct aactcacttg aaccttttat
     2581 tacagcaaca ctccacattc ctgcatttac gccttctttt acatcactta ttgtatctcc
     2641 tacttttacc atagaactca taggagacac ccctaaagct tcagcatttt tatagcacat
     2701 ccaaggatac ggtcttcctt gagatacttc tgatggtgtt acaagaaaat cgggtgagta
     2761 tcccttcttt gctgcatttg gttcaactat gttcatcatc tctcttgtat aacccgttgt
     2821 agaacctatt tttaatccat tttttctcaa cttttcaata gtttcaacaa catgaggaat
     2881 tggagtcgta tactcttcta atgtctcaaa taacattggc tcaaattcag cataaagttc
     2941 atttacatca tcttcagttg gcactttacc aaacttatct gaccaaatat tctttattct
     3001 atccatttca cacatttctc ttatatggtc tatttttaat tttcccatag gttttcttgc
     3061 ttcttccatt gttacatcta tacctcttct tttaaatatc tctataaata cattaagagg
     3121 tgcaaaacat ccataatcta ctgttgttcc agcccaatcg aatactactg cttttatttt
     3181 ttctccatat atctttttca tatttatagc ctccatagat tctttttaga atttttgtgt
     3241 ttttaattgt ttttactttt aaagtataac tttcatatgt taattataca tcatatttag
     3301 gttaaatata tattaaattt ctgtttttag ttgtatttag ttgtttttat tttgtatcat
     3361 attcccataa aatcctaagt tacaatctaa aatttacttt ctaatcttaa tctatgaact
     3421 gactatattt tatgaattta gttgtattga gcatagttgt attatataaa actaagggtt
     3481 tattttgctt ctatccatgt tatatttatc tcatttaact tatccttcca ttcattcgga
     3541 ggttctacat cacaaataac aaaatctaat tcctctaaat ctgctatctt atacatacta
     3601 gcaattccta ttttagaact atcacataat acaaaacttt cctttgaatt ttttatatat
     3661 tcttttgaca acaatgcctt attttggtct atactactaa ttccaaaact atcaagtata
     3721 ccttcactag atataaaagc tttatctacg tataagtttt tcatagtatc tattgacatt
     3781 ggacatgctg tcctttggtg ttttaaattt acttttccac caataaatat aacttctcca
     3841 tcaaacaaac cttgattctc ataagaaatc aattttgata gaactggata agagtttgta
     3901 ataattttta aattgtttac agatattata taatctacaa tctgcaaatt ggtagtacct
     3961 tcatctatga taattgactc attgtcattt acaaacttag ctgcatattt agcaatactt
     4021 tgctttttat ctaggttagt attgtttctc tttatatgtt ctggttcaat cactccataa
     4081 tttattttta tagcaccacc atataccttt tttaatttat tttcattttc taattcttcc
     4141 aaatatcttc ttattgtttc tgatgatact tctaatcttt ttactaattc tttagtgttt
     4201 accttccctt ttgaatctaa taatttaagt atttcttttt ttctatctat cgaatttaaa
     4261 gacattcatt ttcctcctaa atataatata tttttctcct ataatattac aatgatacct
     4321 aagatttatg aaatcatatt tatgtattaa attttttact taattaaagt tattttcaaa
     4381 agactttgta ttatcattaa aaaagtccat caaatattag tatcagatgg actttgtaaa
     4441 attttatttt ataaattaat atttaaagtc taactactaa tttattttct tgtctccaag
     4501 aattttacta aattatttac agtagacata tcttttctct ctaaatcagt tggttgaagc
     4561 tctatcccta atctattttc aatttcaagt aaaacttcaa ttatagctaa tgagtctaaa
     4621 agttccgtct caaataaatt aaggtctaaa tcttctctta tctcatcagt ccctaataca
     4681 tcttcaaata tttctataac tgtttcttgc ataaattttc ctccttaaaa ttaagctaaa
     4741 taacctgaga atatcagcaa tccaaaacac acaatatgga atgtaatgaa aatctgcact
     4801 ctctcgaacc atttatcttt tttatgtttt ttataaaatt tagattttcg ctgatatata
     4861 tcagttaaca caagtgccaa tccttggtat acaccataag caacataaca ccaagttaga
     4921 ccatgccaaa atcccattgt tatcatggtt atcatctgag ccacatgagc agcagtagtc
     4981 cttttcttaa atcttttctt tctcattgat gacataacaa atctagaaaa aatataatct
     5041 ccaaaccatc ttgaaagact tatatgccat cttgtccaga actctttcat atctttgctg
     5101 ataaatggct tgtcaaagtt tataggtact tgtattccaa atatgtaccc tgtacctata
     5161 gcaaataaac tatatcccgc aaaatcaaag aacaggtata aactatatgc gtacatataa
     5221 cttaaaatat ttataaaact catatcttta ggtattcttg atacccaata tgtatttatc
     5281 aaaaatgcta taacaaactt ataacctact cccatgacaa tattttttat tcctggcagt
     5341 aagtattctt ctatatactc ttttctagag atttgttttt ccaagtcctg ttcaaatctt
     5401 cttgacctat caataggtcc agaacttaaa gttggaaaaa ataacataaa ataaagcatc
     5461 tttgaaattt taacttcttt tatagctcca tcataaattt caataaccat ttgtatagtt
     5521 ctgaaattta agtatgatat tccaataaat ccaattattc caaaagaagt aactggagat
     5581 attttattta ttattattgg gagcattgat gcaaacaaga atccccaata tatgtattta
     5641 tttttagttt ttcttcttac atattcatat cctttaacta ttattacttc aagaataata
     5701 aatattacca agtactttag ttggacatca attcctacta ttaagaatat cataaacaaa
     5761 cttgccaaca taccataata ctttatattc ttgcccatta gacctaaaat tactgctggt
     5821 attgatgtta ataataatat ataaagataa aaataatctc catattgtga aaaaatcatt
     5881 aaatttcctc cataagtttc tttctgtcta ttttaccatt gatatttgtt ggaaactcag
     5941 agattattga aatatttctt ggaatcatat atgaaggtat atactttcca agttcctttt
     6001 ttattatcat cccattttta atattactta aatcattctt ttcatttaac tcaacaatac
     6061 cttttaagta agcaattttt tcgtccttat atactggtaa aactactgca ttttttacat
     6121 tatgtacttt tctcaaatta ttttctatat cttctatttc aattctaaat ccatttaatt
     6181 taatctgaaa atcttttctt ccacaataat atatatttcc atctaaaaga tatcccatat
     6241 ctccagtttt ataagctctc cactttacac catctatttc atcatagaaa aatacttcat
     6301 cagtcttttc tttattattg aagtaacctt tagaaactga tggtccaatt attataattt
     6361 cacccttttc attttcttta agctcatttc catcttcatc aagtattttt attttacaat
     6421 ttgacatagg gtatccaact ggaagacttt tttcatcatc tatagccttt tgagtcatat
     6481 catttacact tacaccaaca gtagcctcgg ttggtccata tccattgata attcttgtgt
     6541 ttggaaatct actcataagt tctttagtaa gattttttga aagtgcttct cctatgaata
     6601 tcattgactc aaggttagga agcattttac tattaaactc tttttcagtt atacatactc
     6661 ctgcaaaaga aggagttgag acccatacag ctatatctga gatactaaat tgtctaaata
     6721 gttctctata gtctgctaat acatcttttg atattgaaaa taatgtagct ccatggataa
     6781 gaccagggta tattgttgta actgaaaggt caaaagaata tgctggttgg ttcattatta
     6841 ctttttcact tccatctatg tttagatatg ggcttatcca atctgaaaag ctatctaaat
     6901 tgtttgaact tatttgaacc ccttttggct ttcctgtact tcctgaagta aatagtatat
     6961 atgcattttc atcatctttt acccagtttt ctttatctaa tgattttccc tgatattcat
     7021 ttattatatc atttaattta tccatatcaa ttacattgat atctccaaaa tttctttcat
     7081 cactaaagtt aaataatacc tttggtttta tctctttagt gacttcaaaa actctatcaa
     7141 ttggaaaact aatatcaaga ggcacatacg ctctacctga ttttaaagct cctatcatac
     7201 atgccattat catgttttct ttatttccat aaattactat aggagtatct tcttctttgt
     7261 atacatcctt tagaaatact gatatagcat ctgaatattc atttaaatct ttataagaaa
     7321 gtttatctcc attacacatt agtgctgttc tatctgtatt tgaatatttt tttattcctt
     7381 caataatttt catataagaa tgctccttta aaatccgtta taaataaatg gcaattcttt
     7441 aaatggagta aatgttagaa ctcctatcgc caatattata accatagtta aacaaatact
     7501 agttaacaaa tctttattgt tctttaaata ttttaaataa tctctcacag acatctaccc
     7561 atcctttcgt accaagatgc attacatccc ttaaataata cttatctgac cctttatctt
     7621 taaggtttag aacttcaaag cctttgcttt ctgctatttt ttgcgcttta tcgtagtact
     7681 gatttctttc tttttcagaa ataccagtca aattatagaa tttatccata cttgggatta
     7741 atacaacaac tggcttaata cctaagtcag tacatacatt tagcatcagt ttataatttt
     7801 caaactcctt agatgttaat aaatttacat ctttgtctct tcctttgtat tgctctatac
     7861 catctttaaa atgctcttta taatagtact tatcaatatt tagtggattt ttaccaactc
     7921 tttttttagc atcttcaatg gctttctttc tctcatatga ccaatttatt ggtctttttc
     7981 tttttgttgc tctcttttta tctagcttaa ttaatcgttt ataaagaata cccttttctt
     8041 taagagctat acaatacttt cttccttgaa aataaggctc taaaagcacc ttttcagctt
     8101 tttcaggaaa tgtcttaggc tcatatagct tagcatataa tgcttctgct ttatactcgt
     8161 cacttcccca taataactta cttgattttt ttgcatattc aatcttattt tgtttagata
     8221 tttttggatt atctaaaaat cgataaaatt gtattggtga aaatctagac tgataatggt
     8281 gacttgtgac tccgtctttt tccataaacc attgcataga aattaacaag acaactttct
     8341 tattatcaat attaggattc atacttccta atattgcagc atcttgtaat gtctgagtat
     8401 aggctctccc aattgcaaac accttatttt tactcctatt tgtattaaaa tagtactttg
     8461 gatgttgttt tgttgaatta ctaagttctg atgaccccaa aatcataata tccttttctc
     8521 ttaaaaaatg attatttgct gtaacccctt tatcttttat atcacttaaa gtatcatcca
     8581 taattggaag tagatttttc tccctcaaaa gttcatcagt tttagaatca agaaatttgt
     8641 ctaacccaaa taaaaatact acgcctatta taaaaggtgt aataaagtat atcaattttc
     8701 tcatattttc tctcctctaa aaatattcaa atttatgtta ttattaatca tattagtttt
     8761 gatatgtata ttcaactaca aaattgtaat aaaatcgtca aaactttttt tgatactgtt
     8821 acaaaattgt aaaaaaaaag aaaaaaacgc caaaaataaa tttcttttta gcactttttc
     8881 atacttataa tttgacattt taacacttgg ttaagtatac accttacctt aacttaaaat
     8941 taaatttatt taagaatata tattataaat tcttatattt tctaatataa actatatact
     9001 atcagctgga taaacaatac tactttgtct tcttatctca tccataattt ccattactat
     9061 tattgaattt tcatgagaat ttatatttga ttctatttta ttgttattta taagacttat
     9121 aaactctaca agttcataat acattccttc tatttctcta ggcttttcct ctgctttttc
     9181 ttttggaata gatatatctt cttctcttcc atctctgtat ttaataatag ctttttcaaa
     9241 tagattaagc ttttcaatta ttatacttcc ctcttctcct tgtatttcag atggtatata
     9301 agaatcagct actttagaat actgaattat cgcatccata tcatcatatt gaaatacagc
     9361 tgaaccttct ccatccactc cagtttgaag tatatgagaa actgccttta cagtttttgg
     9421 agcaccaaat aaattcacca taggatgtat acaatacact cctatatcca ttaaagctcc
     9481 atttgacaac tcttttttaa aggcattttc tataattcca tttttatatt tatcatatct
     9541 tgaagaatac tgacaatagc ttccaaaaaa tcttcttatt ttccctattt tatataagtt
     9601 ttctttaact gctttaaagt ttggaacaca tgtaattctc atagcttcca taagcaatac
     9661 attattatct ttggctgttt ttatcattga tttgacttct tttaaattag atgctaacga
     9721 cttttcacaa agcacatgtt ttttgtgttt taaacaagta atggtttgct ttgaatgtaa
     9781 tgcatttgga gaagctatgt atacagcatc tatatccttc gatttagcca tttgttctaa
     9841 atcatcaaaa aaaatacttg ctccatatag agttccaaat tctctagctt tttctaaatt
     9901 tctagaatat acagcaacca attcaaagtc tttaacccta gatgcagctc ttagaaatat
     9961 atttgctata ttgcttgtcc caacaatacc aaatcttatt ttacccatca taaatcactc
    10021 cccattttct acactttatt ttaattttaa taaaaaactt tacaacttat agatatatta
    10081 taaaataatt ttatcaaata aaaaagaaag ctaattttaa atgatttcac cattttaaca
    10141 tagctttctt ttaactacaa tctaaattca acttatattt ttaaagttat ctttctacta
    10201 tatctttatt tcccctcaaa aattttatag taagaatttc atccacaaac aatgttacta
    10261 aaagtatagt tacaacaatt ggccaataag gtattataag actatataaa ctatcaacta
    10321 tatacacttg aaaatatcta actccaataa atgtcaatat agtccaatat acagaaaact
    10381 ttagacatac tattcctcta ctattgtact tataatctga ataatcccaa tagaccttat
    10441 taaaatatac tttcataagt attcctgtaa tatattctac tactgtcggc actactagac
    10501 ataataaaag caatgcaaat atattattgg ggtcaataaa tcttgagacc tctattataa
    10561 tcgacattgc aattgcatac attggtttaa atggaccatt taaaaatcca tcttcttgaa
    10621 aatgcccttg tttataataa ctatataagt tttcaattat ccaacctata aaaccataca
    10681 agaaaaaatt aaataaaaaa tataatagag aacctacatt atccatgaat attcctccta
    10741 gctgtttata cttatttttt ctcatattca tggatttatt ctaaatcaaa gaaattatat
    10801 ttaatttatt taattaatac tttatattat agattgtgac attctttaga gttcaaactt
    10861 tttaggtatt ctctaacttc cagtaaattt tcaagtatct tatgtgattg tgatttaatt
    10921 tgctcatctg gctcttttaa atctggtaca tttatacatc ttataccacc attgtatgca
    10981 gcttcaactc ccataggcga atcctctatg acaatacaat ttttgggatt aacatttatc
    11041 tttttagctg cttttaagaa tatctcaggg tttggtttag agtttacaac atcatctcca
    11101 caaactatag catcaaagta atcttttaaa tttgccttag ctaatctctt tactgctctt
    11161 tctctctttg ttgatgttgc aacagccatt ttgtatccat tttcctttaa aaaagagatt
    11221 agttcattga ctcctaattt gataggagct cccttcctct ccataaattc aatcatattt
    11281 ttagtttttt cgtcatataa atctattatt ggtacagaac tatcgtatat atctgtaaga
    11341 ccttctatta ttcctttacg atttcttccc ataactgatg tataaatttc cttagtcata
    11401 gtatatccat acttttcaaa cgtctccatc caaaattcaa gagaaattct ctctgagtca
    11461 aacaaaactc catccatgtc aaaaataatt ccttctacat tttgcataca attcctccaa
    11521 tattttttga tataattata ttacactttt tatgattaat tttacaaccc tcaatttatc
    11581 caattctaat attgtaaaat tgtaattttc aaaagttaca ctttggttta ctttaatatt
    11641 tgaaccaagc atataggata tccatccacc tatactgtca aatccatcat gctctatatc
    11701 aagtccaaaa tacttattaa tatagttgat agattcagtt ccatcaacta agtaagaacc
    11761 tgctcctatt ttcttaatat gagtctcttc tttgtcaaat tcatcttgaa tttctccaac
    11821 tatctcttct agtatatctt ctattgttac cacaccagat gtaccaccat attcatccac
    11881 aactatagca agttgaagtt tttcctttct tattctctca agagctttat ctatagttaa
    11941 attttcagat atatatataa tatctctcaa tatttcttct agttcaattt tattttcatt
    12001 tatcttttga ttgtataaat ctcttatatg tacaaaacct aatatatcat ctttattttt
    12061 tctgcatact ggatatcttg taacaccttc ctcttttaaa atagctaata tcttttcttc
    12121 agaatcactt tcatatatac ataccatatc agttctagga accattatct ctctaatttt
    12181 cttttcttca aattcaaaaa tattatcaac taatctttgc tctgattcat ctataagtcc
    12241 acttttataa ctttcttcta ctaatagtct tatttcgtca cctgtatggg gttcatcaac
    12301 ctcatcagct tgtgaatatc caaatggttt aagaaataaa tcagtactta agttgaaaaa
    12361 atatattatt ggatatgtta acttataaaa tcctactaat aaaaaagatg tactcaacat
    12421 tattttttct gtattataaa gtgctaaagc tttaggaacc aactctccta ttacaacctc
    12481 catcattgtt ataattaaaa aagaaatgga aattgatata gtatatataa tactttcact
    12541 aagattaaat acacttacta ttggtaaaat taactcttta actgtagatt ctcccatcca
    12601 acctaatgca agtgaacaaa gtgttatacc aagttgacat gcagaaagat aggaatttaa
    12661 gttgtctttt attattttac atctttttgc attattatta ccttcagtta tcatggtatc
    12721 aatcctagaa ctacgaagtt ttaccatagc aaattcacaa gctacaaaaa aagcatttat
    12781 caaaacaagc attactataa gtgttacttt taataactcc cgcataaagt attggttctt
    12841 ataatgtaag aaatctataa gaactcaaag ttcccccctt atattaataa atttaattta
    12901 taatatatat tctatcatat ttattaatat atcctacttt attacaaata atttttataa
    12961 actaaataac cccttttaat atttatgact ggctatttga taaatattaa aggggttatt
    13021 tagttatcat tttttatttc taaaaatctt ttctcaaatg atttagttat ctatttatcc
    13081 aattttttac ataatttaat gattaaaaat attaattttt tcac
//
LOCUS       DAEOSP010000112        13109 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.112, whole genome shotgun sequence.
ACCESSION   DAEOSP010000112 DAEOSP010000000
VERSION     DAEOSP010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13109
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.112"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(195..893)
                     /locus_tag="KRH54_002385"
     CDS             complement(195..893)
                     /locus_tag="KRH54_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8580040.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            complement(927..3629)
                     /locus_tag="KRH54_002386"
     CDS             complement(927..3629)
                     /locus_tag="KRH54_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBG8580041.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKDIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            complement(3807..5624)
                     /gene="ftsH"
                     /locus_tag="KRH54_002387"
     CDS             complement(3807..5624)
                     /gene="ftsH"
                     /locus_tag="KRH54_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBG8580042.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFEESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            complement(5655..6098)
                     /locus_tag="KRH54_002388"
     CDS             complement(5655..6098)
                     /locus_tag="KRH54_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8580043.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            complement(6589..7479)
                     /gene="metA"
                     /locus_tag="KRH54_002389"
     CDS             complement(6589..7479)
                     /gene="metA"
                     /locus_tag="KRH54_002389"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBG8580044.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(7509..8774)
                     /locus_tag="KRH54_002390"
     CDS             complement(7509..8774)
                     /locus_tag="KRH54_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBG8580045.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(8884..8986)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9040..11697)
                     /locus_tag="KRH54_002391"
     CDS             complement(9040..11697)
                     /locus_tag="KRH54_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBG8580046.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGVFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     gene            complement(11825..12568)
                     /gene="yaaA"
                     /locus_tag="KRH54_002392"
     CDS             complement(11825..12568)
                     /gene="yaaA"
                     /locus_tag="KRH54_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBG8580047.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLQVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFDLEGYKYKENLSNNSEYIFVKE"
     gene            complement(12578..13042)
                     /gene="bcp"
                     /locus_tag="KRH54_002393"
     CDS             complement(12578..13042)
                     /gene="bcp"
                     /locus_tag="KRH54_002393"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBG8580048.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
BASE COUNT         4059 a         2404 c         1507 g         5139 t
ORIGIN      
        1 ttacactata tacttatttt aaaacatatt tcatattatt gatactaaaa aattattgat
       61 actaaaaaag ctaaaaaata aaatgcatgt gctaaactga caacaagcac atacacttca
      121 ttcttatttt aactaaacta tataatttta atatatatta ataagcacat aatctaataa
      181 ttaatatatt acaactattc atcatttaat ctgtatccta caccaacttc tgtaattata
      241 tatcttgggt ttgcaggttg tttttctatt tttcgtctta acgttgccat aaagaccctt
      301 aaagacttag tctcattccc tataacacta ccccatattt cttttattat aaaattatga
      361 gtaagcactt ttccatgatg ctttgcaagt aatgaaagaa tattatattc aataggtgtc
      421 aaatggactt caacatcatc aacaataact tttcttttat caaaatcaat caataattta
      481 tctacttcaa acgtagattt aacatcatct ttttgttgag cttctacttg tgaatgtcta
      541 aatgctactc ttactcttgc taataactct actatggaga atggttttgt taagtaatca
      601 tctgcccctg catcaaatac ttctaccttg tctctatctt gctctctagc tgaaactaca
      661 attattggta tatttgaaaa ttgtctgata gcttttatta cctctattcc atccatatcc
      721 tctaagccta aatctaaaag aacaacatct ggagaatatg agacagctat agatatacct
      781 tcttttccag ttatagcctc cttgacatca taatcctgtg ttaaaagtgc agtagatata
      841 aattttctta ttgtcttatc atcctctact aacaatataa gttttttact catttcttcc
      901 tccaaaacta attttaaaat ttaaaactat tcattttcct taggcaaact aaacttaaaa
      961 gttgaaccct tatctttatt gttttctacc ataatttcac ctttatgagc ttgaattata
     1021 gatttacaaa ttgataatcc aagtccaaca ccctttctgg aatctttagc tccttcttct
     1081 ccagtaaaaa atctgtcaaa gatatgcttt ttaagttcct ttgatatacc aggaccatta
     1141 tcagatactt caaaccaaac ataatcatct ttctcatata catttatctc tatcttacaa
     1201 tcttcctttg aatatttcaa agaattatct attaaattaa ttaaaacttg ttctattaat
     1261 tttgcatcca taggaacaaa taatatctgt tctggtatat taatatctat aagtgcattt
     1321 tcaattcttt tttttatttt ttgtaacgct tctgatataa tttcctcaac cagttcagaa
     1381 tgttttttaa cttccaattt cccttcatct atcttggtca tactaagtaa attttcaaca
     1441 agtcttatta accactgagt atcttcatat acaccactaa gtaattcatc tattatatct
     1501 tgacctatag tacccttgtt ttttataatt gtacttactg caccagatat tcctgctagt
     1561 ggagttctaa ggtcatgcga aactgctctt aaaagattac ttcttaatct ttccctttca
     1621 atctcaagat tggtattttc tttagcttct gacaagattt ctctatcaag cgctattgcc
     1681 atttgagcaa ttattgtttc aattaagctt atatcttctg tatccaattt tgaatcaata
     1741 catgatattc ccactactcc taaagttaaa ttcattccta caattggtat atagtatccc
     1801 tttgaaccag gaagtgtatc cgtgtcattt cctgccactg ttgaattgga taaaacccaa
     1861 cttgcaacag ctttttcatc ttcactatcc aaatcatttt tatccttatt attagtattt
     1921 tttttgtaaa gctttgattt attatgctta tctacattta aataacaaat tacatctcta
     1981 cttaagctaa gagacaaaag ttctatacct ttgtttacta tatcatcttt atttgataat
     2041 cttaaaaatc ccttaattat ttgatatatc atttttgtat tttcttctct tttagcagca
     2101 gtttctgctt catgctgtat cttagacgta agagtgctag ttattatacc tactattgag
     2161 aatacaacca gtgttactat atagtttgaa tctgctatag ataatgtata taatggtgct
     2221 gtaaaaaaat aatttaaaag aactatatta aatacagaac ttattatacc tgtactatac
     2281 cctttagtcc acatagaaac taaaactaca cccaacatat atattaacaa tatattttct
     2341 cttataaatc ccatgctttg tactgcatat gctaaaatac ttatcatcaa agtaataaat
     2401 ataagtttta taaaatcaac tttgctaatt ttaaatttag attttattga tgatttatcc
     2461 tttttaggtt tgtatttaac atctgaggca aatttatatg gaattatatg tatatctata
     2521 taatcaactt catcaattaa tttgtcaact atatcttttt taaatctttt actaaatctt
     2581 ttattacttg aatgatttct acctattaca atttttgtta catttctcag ttttgcaatt
     2641 ctaagtattt gctctattat attttcacca tgtaaaacaa ttatttctcc acctaatttc
     2701 ttagctaatt ttaggttatt ttgtagttgt ttttgttcat caaatggaag tcttgctgtg
     2761 tttggagttt caacatataa agctatccat tttgcaaatg tactgtctga tattctggat
     2821 gcagttctaa taacttttgc tgaagatgga gatggactta tacatgcaag aagtgtatct
     2881 gatgtaggta ttacagttac atcacccttt gatagtctgg tcatttgaac ttctttatta
     2941 actctctctg ctgtttttct aagtgcgatt tctctaagtg caaccaagtt atcttttata
     3001 aaaaaattat tttttgctct agtagcttgt tctttcttat atatcttacc ttcattgaac
     3061 ctttcaagaa gtacatcagg ttcaacatct ataagttcta actgtgtatt tgtatccaaa
     3121 aacttatctg gtatagtctc tttaactgat acgtttgtaa ttatctcaac aatgtcattt
     3181 agactttcta aatgttgtac atttacagta gtgtagacat ttatgcctgc taacagcaat
     3241 tcttctatat cttgccatct ttttgtatgc cttaacccag caacattaga atgggcaaat
     3301 tcatctacta atataacttc tggttttctt gctaaagcag catctaaatc aaattctttt
     3361 agttttatag atttatattc tatatccttt acaggaagaa tttccaagcc atcaagtaaa
     3421 gccaatgttt ctggtctagt gtgtggttca atatatccaa ctactacatc cacacccact
     3481 ttttttaaat tttgtgcaga ttccaacata gagtaggttt ttcctactcc agctgcataa
     3541 ccaaagaata tttttaattg acctctactt agtttttctt cttcattttt aattttttct
     3601 aatagaatat caggattagg tctatccatt aaaaaacctc cttacaccat tcattactat
     3661 tataatatca attccgatat aaaattgtag ttaagatgta tttttaatga ttaagattat
     3721 gtaaagattt aaatatgaat ttaaaaaatt tagttgtaaa ataaaaatgc ctaacaatag
     3781 gcatttttat cttgtgtata atcattttag tttaagttaa gtatttccat aaactcatca
     3841 cctgtaatag tttctttttc taataaataa ttagcaagtt catgcaattt atcaatatta
     3901 ttttctaaga tttcaattgc atttttgtga caagccttta ctattttaaa tacctcttca
     3961 tctattttag cagcagtgtc ggaagaacaa gcaagagaag tatctcctcc taaatattga
     4021 ttgcttacag tttcaagagc gaccatatca aactcttcac tcataccaag gcgagtaacc
     4081 atagctcttg caattttagt agcttgttct atatcatttg atgctcctga tgtacaagtg
     4141 ttaaagataa cctcttcagc agcacgacca cctgtaaaag tagttatttt atcaattgct
     4201 tcttctttag acataagtac actctcatct tcagcaactt gcatagtata tcccaatgct
     4261 cctgaagttc taggtattat agtaatctta tggacaggtg cagaatgttt ttgcatagca
     4321 gcaacaagtg catgacctat ttcatgatat gctataattt tcttttcctt atctgaaatt
     4381 acagctccct tacgttgata cccagcaatt acagtttcaa cagattcttc aaaatcctgt
     4441 tgttctacta tatctctgcc agacttaact gctaaaagtg ctgcttcgtt tacaatattt
     4501 gcaagttctg cacctgatgc tcctgatgta gaccttgcaa ttgcattata atctatatct
     4561 gagcttacat ttacattttg agcatgtacc tttaatatag cttcacgtcc tccaaggtct
     4621 ggaagttcaa ctggtatacg tctatcaaat cttcctggac gaagtaatgc tttgtcaaga
     4681 ctgtcaggtc tatttgtagc agcaagtatt actactccaa ttccaccatc aaaaccatcc
     4741 atctcactta acaactgatt taaagtttgt tcacgttcat catttccacc taatccactt
     4801 ccattatcac gctttttacc aatagtatca atctcatcta taaacactat acatggagct
     4861 ttttcttttg cttgtttaaa taaatcacga atcctagaag ctcccattcc tacaaacatc
     4921 tcaacaaact cagaaccaga tattgagaaa aatggtacat ttgcttcacc tgctacagct
     4981 tttgcaagga gtgtttttcc tgtacctgga ggtcctacta aaagtgctcc tttaggcatt
     5041 tgtgctccta tctttttata cttttctgga ttgtgcaaga aatctactat ttcagttaaa
     5101 gcttcttttg cctcatcttg accagctaca tcattaaatc tttttccact ttgagcagat
     5161 acatacattt tggcgttgct ttttccaaat tgcatagcat taccacccat tcgcttttgc
     5221 atagacttca tcattaagtt accaaatagc ataagaatac ctataggtat tatccatgta
     5281 agtaaaatac ttaaaagagg tgaattttcc ttaggaataa ccttagtaaa ttctactttt
     5341 gcatttttta gtctatctac caattctggg tcatccattc taccagttat gtatatattc
     5401 ttatccttgc tactagatgg atttattgct attctattat tttctatctc aacagattta
     5461 acctttcctg tatccaacat tgtgagaaac tttccatagt caatctcaac cattttttta
     5521 tccattaaac ttggaaatat taaagcattt aaaaacatta ctactattat tataactgta
     5581 taataataga gtgctgacct tttttggggt ttatgttcat ccataatttt attctccttt
     5641 aaattatttt tatgttattt gcaatcaatg taagttaaaa gtttatttaa tttctctaaa
     5701 ccttctatta tatcttccat atcttgcaat ttttcactgg tcatattttc aaaataacta
     5761 tcaaaaatac tacttgaaat atgttcttgt atatccaatg atttatttgt aacttttact
     5821 tttacaattc gttggtcttc tatatctctt atgcgattta aaaagccatt tttttcaagc
     5881 ctttgacaga ttactgatac attactattt gtcatgttta agttcttccc taaatcgctt
     5941 atttttttct ctccatgttc acataactca gataaaatca aagcttgcac tgctgtaata
     6001 ccaagtggct gataaaaatc acttaataaa gttgtaaatt tcatatttat atctctagtc
     6061 aactctagta gctctttctt aaaactcatc tcatccatac aaacgctccc tacgaaaata
     6121 ctttatttca aatttaggct gaagtgcata ctgtaaataa atacttaaca ctataatgtt
     6181 taatgtatta aatattatag tattaagtat ttattgtcaa ttacaatatt gtaacttcaa
     6241 ttggaatttt gttatcttat tgatatttaa ttttatacag gatattttta ataaaattta
     6301 ataaaaaatc tatatatata ttttacactg atttctccct ttttaatctc ttttaaaagt
     6361 taaaatacaa atttattgat actataaagg tttagtctcc cattatcgca ccatatataa
     6421 aattaaagcc acctctatct acatacacct aaagtgattt tcttaaaata taaaactact
     6481 attataaggt caaatttaaa aaaaataagg tataaagtat cattaagatt aactttatac
     6541 ctataaattt ctatattttt ttagttaaca tttatatact cttaaaattt attgtatcaa
     6601 atagtttttt atccaatttc taaacaacaa ttctgaatgt tttttccatt ttacagttgg
     6661 ctcttctgaa gggtcatcat taatatagta attttgaggt atagatattt tatctccttt
     6721 atttatatcc ctcttatatt ctttatcaag agtgtatttg tcatactcgc tatgacccat
     6781 tacgaacaca tctcttgaat ttgtaattat atagggacca gcttctttag aatgactcac
     6841 aatgctcaac tctttattct ctttcaaagc ttcaatattt acagtagtat gtcttgaatg
     6901 tggagcaaaa aattcatttt caaatccatc tactatttta ctatctttat ctaccttatg
     6961 cttaaatact ccaaaacatt tttgacttag tggaagtttt tttacattgt agtatttgta
     7021 aagacttgct tgagctgccc aacatatata taatgtagat ttagtttttc tcttagaata
     7081 atccataatt tcttctagtt cattccaata atcaacttct tcaaactcca tttgttcaac
     7141 tggagctcct gttattataa agccatcaaa atactctcct ttggcttcat caaatgtttt
     7201 ataaaattct ttaacatatt cattacaact tcttttactt tctcttgttg aaattttaat
     7261 aaattccaca gacacgttga atcctgtttt atctatcctt cttagtaaat ctaattctgt
     7321 atctatcttt ataggcatta gatttagtat agctatctta gtgtctatgt tttcttcata
     7381 ctttaattcc tttttaaaat cttctttata aattacatct attccttcat ctaataattt
     7441 attaataaca ggtaatccct ttggtagtat taaagccata tcttttcact tctctttctc
     7501 ttagaatatt agaaatttaa agcttgattt aaatcagcta ttaaatcctc tacattttct
     7561 attccaacag ataatcttat tagtgaaggc aatactccag atgcaattaa ttgttcttct
     7621 gttaattgtc tatgagtagt tgaagctgga tgtataacac aagttcttac atctgaaaca
     7681 tgtgtaacca aagatgctat ctgtaatttt tccacaaatt ttttggcatt ttctaaccca
     7741 cctcttactc caaatgataa aacaccacta catcctcttg ataaatactt tttggcattc
     7801 tcataatact tgttatcttc aagtcctggg taattaatcc aatctacatt ttcatgtttt
     7861 tctaaaaatc tagctatttt aagtgcattt tcactatgtc tctccattct aagatgtagt
     7921 gtttcaacat ttaaattagt aagatacgca ttgaatgggc ttaaacaatt tccatagtct
     7981 ctaagcaact gaactcttgc tttagttgca tatgcggcat ttccaaattt ttgagtatag
     8041 cttataccat gatatgttgg gtctggctca actaattctg gaaattttcc attatcccag
     8101 ttaaagtttc caccatccac tataattcct ccaacacttg aagcatgccc atctaaatat
     8161 tttgtggtag aatgagtaac tatatttgct ccatacttta aagggttaca aagcactgga
     8221 gatgctaatg aattatcaac aataaatgga acattaattc tcttagctac atttgctatt
     8281 ttttcaaaat ctatgacttc aagagttgga tttgcaagtg tttctgcaaa cacaacctta
     8341 gtattttcct ttgcaagttc tactatctca tcttcacttg aatctaagtc aaacgatatt
     8401 aaatctatac caaatttttt aagactaggt cctagtaaat tgaatgttcc tccatatact
     8461 tttgaagaac aaagtatact atctcctgat ttacatatat ttaaaactgc caacatattt
     8521 gcagattgcc ctgatgatac agctacagaa gatactccac cctctagtaa acttattttt
     8581 tcttcaaaag cttgaatagt aggattgctt attcttgaat atatatgtcc atcaactttt
     8641 aaatcaaata attcagcaag ttggtctata ctgctatatt taaaagttgt actttgatat
     8701 aaaggtagta ctcttggttc tccattacct ggtttataat ttccttgcac acatattgtt
     8761 tctttattat acattcttgt atccccctta gattttagta aaaaaaatta aactatttac
     8821 cagatgagag ttaaaataaa taaagcctga gtatataact caggctctgt ataagcttaa
     8881 ttatactcat ctctcagctt tcgccgcagg atttagcacc aatgtatata aatatactgg
     8941 ttgccgggtt tcatagggcc agtccctcca cctctctgga taagatttat ttaatttttc
     9001 tatgttttat atcttatata tttttcctaa ttttgtcaac tattttttta atttttttct
     9061 tttaataatt ttcccaacct ctacttgaat tacagacatt atggctaaaa caatcgttat
     9121 aagccactct aagttattta aaactgttaa gttaaaagca tttctaaaaa caggtacaaa
     9181 cagtattact gctattaaaa atgcagaagt taaaaatgag attaaaagaa atggattttt
     9241 accattattt ctattaaaca ttgaatctgt atttgaacgc tgactaaatg ctcgtaacat
     9301 ctgtgaaaat gctaatacac aaaatgccat tgtttgacct acagaatgat tttctatatt
     9361 aagaccaatt ttatatgcta aaatagtcat tattgccaca aatacacctt gggtaacaac
     9421 tctttgtata agattttttt caaacaaagt ggttgatttc actggctggt gcttcattat
     9481 atttctgctt ggtgggtcaa ctccaagagc aagggctgga agcgtagcag ttgcaaggtt
     9541 tacccataga atatgaacag caagaagcgg agtatcccaa tttaataata ttgctacaaa
     9601 caacgtaagt atctctgcta tattaccaac aagcaaaaat tgtataactt tttgtatgtt
     9661 tctatataca cgtctacctt cttttattgc ataagcaatt gttgtaaaac tatcgtccat
     9721 taaaatcatg tcagaagaat cttttgcaac atctgtacca cttataccca tggctatacc
     9781 aatatcagca gactttaagg caggagagtc atttacacca tccccagtca ttgctggtac
     9841 ttctccaatt ctttttagag attgtataat tctaagttta tcaaatgggg atacacgagc
     9901 aaatactgta gtatttttaa cagcttcatc tagttcatca tcactcaaat tatcaagttc
     9961 atcaccagat atcacagtat tttcttcact ccatattcct aactcatgag cgattgttgt
    10021 ggcagtaact ttatggtcac ctgttatcat tatggttctt atacctgctt ctttacaagt
    10081 acttactgca tcaattacct cagttcttgg tgggtcaatc ataccaacca tacctataaa
    10141 tgttaaatca tattctatat tttcactatc ttcttttggt atagatgaaa tctcacgctt
    10201 agcaaaacca agtacacgca atgcatcttt agacatgtta agacataatt cgtgtatatt
    10261 ctttttatcc acttcagtaa tgtttctttc accttttgaa gttaatattt tagtacatag
    10321 gtcaagaatt tcgtctactg ctcctttagt ataagcaata atcttttcat ctatattatg
    10381 aacagttgtc atacattttc tatctgaatc aaaaggctgt tcaaatacac gtggatattc
    10441 ttcttcaaga gcttcttgat ctattccaaa ttttttggcc ataaaaatta atgcgccctc
    10501 tgttgggtca cctaatattt catctgtttt atctggatta atacatgcat cattacaaag
    10561 tgcaccagca taaacaatct ctttatacac taatggatgt ttttcagatg cagtatctat
    10621 tgatgtcact tttccagatt caaaatctcc attgacagca atatgtgtaa cagtcatttt
    10681 atttaaggtt aatgtaccag ttttatcaca acatattact gtagctcctc ctaatgtttc
    10741 aacagcaggt aattttctaa ttaaagcatt tcttttagcc atacgctgta cgcctaaagc
    10801 cattacaata gtagctgttg caggtaatcc ctctggaata attgatatgg caagggaaat
    10861 tgcaatcata aacattggca agagaggtct ctgataaaat gcacctattg caaatataac
    10921 aacgcataca ataataccta tgacacttaa tgtctttcca acagtattaa gtttacgttt
    10981 tataggagta tcaaaatcat cttgtgaatc taaaagatgt gctatgttac ccacttctgt
    11041 atccatacca gttgctacta caacacctac acctctacca tatgtgacta ttgaagatgt
    11101 atacgccata ttgctacggt ctcctaatac acattcttta ggtaagattg tatctgcatc
    11161 tttttctgat ggaacagatt ctcctgttaa agaagcctct tgaattttta aacttgatgt
    11221 ttctattaaa cgaaggtctg ctggaaccat agctccatct tctaaaaaaa caatatctcc
    11281 tactacaaga ttacttgctg gaataacatt ttcttcacca tcacgcataa cacgagctgt
    11341 aggtgaattc atatttttta atgcttctaa tgatgattct gcttttttct cttgaacaat
    11401 tccaataact gcatttactg ttacaattat tagtattaca aaagcttctg taaattcgcc
    11461 aaatatcaaa gataatattg ctgctcctat tagtatcata accatagcat ctgtaatttg
    11521 actccaaagc atttgtaaag ttgttctagg tggttttttt ctaagttcat ttaagccatt
    11581 ctctttaagc cgtttttccg cctcggtatc ggtgagcccc tcctctgatg aatctagact
    11641 ttctaaaatt tcatgcgttg ctcttgaatg ccaaggcatc tgtttgtgat tttccatttt
    11701 ggaagaccac tcctttcttt ttttaattta agaataatta tattttaaaa tcaagttcaa
    11761 ttcaatacac actaagtata ttttacctaa atttaacaat gccatttaat atttaacaaa
    11821 acttctactc ttttacaaat atgtattcac tattgttaga taagttttct ttatacttat
    11881 aaccttctaa atcaaacttt ttcagttctt caatatctct tatcctattt tttacaatgt
    11941 attttgtcat catacctcta gctttctttg cttgtgtaga ttccactttc aatatgccat
    12001 ttttatttac tttaaaagta catgtaatat atgtatcact atctttaata aatttttcta
    12061 tggatttgct gtattcattt gatgaaagat taacgatagc tgttttttct ttgattagtt
    12121 ctttatatat acgatttccc caatattcgt ataaattctt aaactcacct acttgtaatt
    12181 ttgtctgcat ttctaatcta tattcatata tactatcata tggttttaca acaccataca
    12241 atccacttat gattcgaata cagctatttg caaactcttc atctttcctt gtaaaatttt
    12301 ctgcttctat atttttatat tgtattccat cataagttaa gattgcaggg tttcctaatc
    12361 tatcaaattt aatgctttca aatctatttt tatttaatac agctaatttt tcattaattt
    12421 tcattttatt tttaaaatct tctataccaa atgtctttat attttccact atttcttttg
    12481 tgttatctat aaagcaaggc aaactcaaat ctctatcaaa tacttctata ttcttcatat
    12541 ttttagctgg tgataacatt ataatcataa attcttatta ctcctgtttt tccaaatatt
    12601 caagaatctc ttgtgcattt gtgtctggtt ttgctttttc aaagactttt tcaataattc
    12661 cattttcatc tataatatat gtcgcacgta caactcccaa tgcagttttt ccatacattt
    12721 ttttctcttt ccatacatca aaagctttta tagcaactaa gtcagggtca gaaagtaaaa
    12781 taaaaggaag ttcatgcttt tctgcaaatt tttgatgtga ttttatacta tctttactta
    12841 tcccaataac ttgtatatct tcctttttaa atccatcata tgcatttcta aaagcacaag
    12901 cttgacgtgt acatcctggt gtattatctt taggataaaa atatactact acttttttgc
    12961 ctttaaaatc tgacatactt accttatttc catctttatc ttctagtgta aattctggtg
    13021 cttttgttcc tatactcaac ataaatatct ctccttttgt ttttattaat aattttatac
    13081 tatgcttata ttattaccct ataaatcta
//
LOCUS       DAEOSP010000113        12939 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.113, whole genome shotgun sequence.
ACCESSION   DAEOSP010000113 DAEOSP010000000
VERSION     DAEOSP010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.113"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            168..1361
                     /locus_tag="KRH54_002394"
     CDS             168..1361
                     /locus_tag="KRH54_002394"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBG8580049.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            1567..2685
                     /locus_tag="KRH54_002395"
     CDS             1567..2685
                     /locus_tag="KRH54_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBG8580050.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDIVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            2816..3061
                     /locus_tag="KRH54_002396"
     CDS             2816..3061
                     /locus_tag="KRH54_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBG8580051.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            3048..3794
                     /locus_tag="KRH54_002397"
     CDS             3048..3794
                     /locus_tag="KRH54_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG8580052.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENIFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            4077..4952
                     /gene="hslO"
                     /locus_tag="KRH54_002398"
     CDS             4077..4952
                     /gene="hslO"
                     /locus_tag="KRH54_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBG8580053.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFICECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            5343..6143
                     /locus_tag="KRH54_002399"
     CDS             5343..6143
                     /locus_tag="KRH54_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG8580054.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            6670..8910
                     /locus_tag="KRH54_002400"
     CDS             6670..8910
                     /locus_tag="KRH54_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBG8580055.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            9324..10859
                     /locus_tag="KRH54_002401"
     CDS             9324..10859
                     /locus_tag="KRH54_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG8580056.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            10875..11636
                     /locus_tag="KRH54_002402"
     CDS             10875..11636
                     /locus_tag="KRH54_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580057.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            11974..12843
                     /locus_tag="KRH54_002403"
     CDS             11974..12843
                     /locus_tag="KRH54_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG8580058.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
BASE COUNT         5043 a         1310 c         2470 g         4116 t
ORIGIN      
        1 tttattctca aaatatgcat attaatttaa taaaatatac caaaatggaa ataaaaaata
       61 aattttatga gaatttaata actaataatt aatatattta ggttataata aatatgtgcg
      121 taaaatctaa caataagcaa taataccgta aatttaggag gaataatatg gatactttaa
      181 aggaactttt taaaatagga agtggacctt caagttctca tactatggga ccacaaagag
      241 cagcagagag atttaagaat gaaaacccag atgcagaaag ttttagagca atattatatg
      301 gtagtttggc agccacaggg aaaggtcatt taactgacta tataattgaa aaaactatag
      361 cacctaaaaa agtcgaaatt gtttgggaag aagatattat aaaagatttt cacccaaatg
      421 ggatgaagtt tgaagcttta gataaagata aaaatgtaac agctgaatgg actgtatatt
      481 ctgtaggtgg agggactata gcagaagaag gccaaagaaa cagtaaaagc aatagtatat
      541 atcacttaga tactatggat gagatagtaa aatggtgcaa agaaaataat aagactttgg
      601 tagattttgt gttggaatgt gaaccaaaag acataaaaga ttatataaaa actataaaag
      661 atgctatgag aaaatctatt gatgatggac taagtacaga tgaaataata ccaggcaagt
      721 tattattaaa aagaagagca agtacatttt ataatgcata taaaaaagat aagagcttct
      781 caacacttgt atatgcatat gcattagcag cttcagagca aaacgcttct ggtaatataa
      841 tagttacagc accaacttgt ggttctgctg gagtaatacc aggaatattc tttgcaatgc
      901 aggatttcta taattatgat gacgaaaaaa taattgaagc actacttgta gctggaataa
      961 taggtaatat aataaaaact aacgcttcaa tatctggagc agaggtaggg tgtcaaggtg
     1021 aagttggagc cgcttgttct atggcagcag cagcagtagc atacttaaaa ggtggaacta
     1081 tagaccatat tgaatatgcg gcagaaatag cacttgaaca tcatttagga atgacttgtg
     1141 accctgtata tggatatgtt caaattccat gtatagaaag aaatgcaatg gcagctcaaa
     1201 gagcttatga tgcagctaat tatgcattat taactgatgg ctcacattct gtctccttag
     1261 accaagttgt tgaaacaatg aaagaaactg gtatagatat gatggataag tacaaagaaa
     1321 cagctaaggg tggacttgct aaacatttct tctcttgcta aaaataaaat tttccaataa
     1381 taattcctct gttttgattt atgaaacagg ggaattatta ttaatggtga attatttaat
     1441 aatgcaaaat atttttatat tcaatttagc tttttatttt taattagtat aacatgctat
     1501 ataatatttt aattataaag aaatatagaa atatttttta ataaattaga aattggagga
     1561 gattacatga gagaggcatc atatttacaa gaacaacttt ataaacatag gaagtcatta
     1621 aatcctattg cagagattgg gagaaaagag tataagactt caaaatatat aagagattat
     1681 cttgataaat taggaattga gtatgaagtt tatttagaaa caggtatagt aggtgtaata
     1741 gaaggtaaaa gacctaaaaa agatattgct tttagagctg acatagatgg tcttcatagt
     1801 gaagaagggg taaaacattt atgtggtcat gatggacatg ctactatatt acttggatta
     1861 attgagtatt taaatgataa taaagaagac ttaaatgata atatagtatt tatattccaa
     1921 ccagcagaag aaggtcctgg gggagcaaaa gctttaatag atgaaggagt gcttagaaaa
     1981 tacaatattg atgaaatata tgggcttcat atatatccag agttaccaga aggttatgta
     2041 ggtacaagag agggctattt tatggctcaa ataggagata ttgacataga aatagtgtct
     2101 aaaagtggac atggggctat gccacaaaat ggtattgatg gtatagtgat agcttcaaac
     2161 tttgtaagct cacttcaaac tatagtgtct agaaatataa gcccaataga caatgcagtg
     2221 ttgactattg gtagaattga agggggagct agaagaaata taatagctga aaatataaag
     2281 atggaaggta ctatgagatg tttcagccca gaagtgtatg agaaaatgaa gcttagagtt
     2341 agagaacttg ctagagggtt tgaattggct tataactgta aggttaatat aaatattata
     2401 gatgattata ttgctgttaa aaatgacaag gatttgtatc aagagtttgt agaggctatt
     2461 ggaagtgata ttgttgtaga gttagagcca cttatgatat cagaagattt ttcttattat
     2521 caaaaagaag ttccaggatt attttttatg ttgggttcaa gaaacgaaga aaaaggattt
     2581 gtaaatggtc ttcataatat aaactttaat tttgatgaaa agatatgtgt aaatgcattg
     2641 aatgtttatt caaaattatt aaaatacaaa gaagcaatag attaggaaga taagttataa
     2701 agattaaaaa gcataaatac ctaaatttaa aaaaagtgta ttaataaata ccccttacat
     2761 atttgtataa atttatttta attgacaaaa atacaaataa gaaggggtga tttttttgaa
     2821 agaaaaagta caaagagaaa agaagataac taatggcaag aatactaaaa tattgacaaa
     2881 tgatgatata atgaaatatg agatagcctc agaactaggt cttatggata aagttggtga
     2941 acgtggatgg gcaggactta cagctaagga agcaggaaga attggaggta tgcttacatc
     3001 acgcaaaaag aggcttaaaa agattgtgga ggagcagaat aaaaaggatg agtcaatata
     3061 gtgattttgc gtttgtatat gatgaactta tgaatgaagt tgattataat ggttgggtta
     3121 aatatataga agatataata gatagtgaag gtgtaaaagt aaaaaatata ttggaattgg
     3181 catgtggcac gggtaatctt acaattccat tgactaaaaa aaattacgat atagctggta
     3241 tagatatatc tgatgagatg cttagtgtag caagagaaaa ggcagaaaaa gaaggagtag
     3301 aattagtttt acttcaacaa gatatatcag agcttgattt tgaaatatct gatttggact
     3361 gtgtactttg tgcttgtgat ggatttaatt atataactta tgatgatgat ttggaaaata
     3421 tattttcaaa gacatatgaa ttattaaaga aagatggaat attcatattt gatataagtt
     3481 ctttttataa attagccaac atattgggaa ataacatgta tggagaaaat agagaagata
     3541 tagcgtatat gtggcaaaat tactttgatg aagaagagaa tctaattgaa atggaactta
     3601 cattctttat taaagatgaa tgtggaaagt ttgaaagatt tgaagagata caccaacaaa
     3661 gagcatatac tgaagatgag atagttgata tgcttgaaaa atctggattt aaggatataa
     3721 aatattatgg ggattttaca tttgacaaac ctagtgaaga aagtcaaaga atcttctttg
     3781 tatgtaaaaa ataagatatt cttgactata taagataatt attaaaaata accatgatat
     3841 aaaaatatag aaaatgcagt tttatagatt aaaagtaaag tgatgatact ttatgacttt
     3901 tattttttaa taattataaa gttctataga taaaaattaa aatatagtaa ctattactta
     3961 gtaaaattaa aataaaatgg gttttgccca aggttaagat agataagtat ttatagataa
     4021 attaaggtag taatataata aaaataagac aattaacggg acatggagga atattaatga
     4081 gagattatgt actaagagcg acatcaggaa atggtcaagt tagagcattt gtcgcaacta
     4141 ctagaaatac agtagaagaa gctagaagat tacatgaaac tacaaaagta gcaacagcag
     4201 cacttggaag aacattaaca gccacatcta taatgggatt aatgatgaaa aatgatagtg
     4261 ataaattgac tgtaataata aaaggtggag gtccaattgg aactattatc gccacatcag
     4321 attctaaggg aatggtaaaa ggatatgtag gaaatccaca agtagaggtt gaagactatc
     4381 caaatggaaa gttaaatgta gcagcagcag taggtacaga aggtgttgta aaagttataa
     4441 aagatttagg tcttagagaa ccatataatg ggacttatcc attggtaagt ggagaaattg
     4501 cagaagactt tacttattat tttgctgtat cagagcagac tccatcagta gtagctttag
     4561 gtgttcttac aaaagaagat gaagtagagt ttgctggagg atttatagtt caattaatgc
     4621 cagatgcaga agaagagact attgctaagt tagaggaaaa tgtagctaaa ttaccttcta
     4681 taactaacat gttaaaagaa ggaaaatcac cagaggatat attaaatatt gttttggatg
     4741 gtttggaacc taaaatactt gatacctgtg aagttgggtt tatatgtgaa tgttcaaaag
     4801 aaagagtaaa aactgctttg gttgcaattg gtaaaaaatc attggctcaa ataatagaag
     4861 aagataaaaa agcagaagtt ggttgccagt tctgtaataa gaagtatatg tatagtgaag
     4921 aagagttgct tgaaatttta aaggaaatgt agactataaa aatagttgta ttgatataag
     4981 taatgttata gtgatatagt aaagtcgtat tgatatagta attaaagtat aatttattaa
     5041 taatagacgt atttatatta aaaaataagt ttaataaatt tttaagctta ataaatttta
     5101 agaattaaga agctttctgg ctgtaatgtt tgcaatgttg cagttagaag gcttttttat
     5161 tttattatag ttcgtgtcaa agattgtatg aaacaataaa atattgtaaa ataatttgta
     5221 tatttagacc tgattggcac aaaggtttat aaggtatttg agttgattat gcgaaattct
     5281 atgaagtagt aaccctgtga ctttaatcat gggaggttca gaagaaggag aaaattttaa
     5341 atatggaaag taaaacgaag tacataggaa taagtatatt aatattattg atgatatttt
     5401 taattgcaaa gaattttaac ggaaaacttg aaatacctat tcctgagagt aatagtagaa
     5461 aatttaaatc agcagcagtg ttttatcctc aacatcaaga tgatgaggtt ttatggggag
     5521 gaagtgccat tgtagatgct ataaaacagt gtggtgttga taatgtatat gtggtattag
     5581 tatcagatgg ttctggagtt aacgttttca aagctaacac aaaatttaga aatttaacta
     5641 ggaagcaaaa ggaagagctt agaaataacg agtttaaatc agctttgaga gagcttggag
     5701 tgaaacctca aaatgtgatt atacttgcag atatagataa aaaggaaggc acacattatg
     5761 aattaatgga aaagactata ttggattttg aacataagtt taaaggcaat gtgacccata
     5821 tagcccatca ctataagtat gatgaccata taatgcatag aaagaatggg gaagttttaa
     5881 agaaacttaa acgtgaaggt aagataaaag atgacttgta ttttgttaag ccaaaatatg
     5941 taaagtatat accaagaaat gaaagagcta tctataaagc aatggatttt gatgactaca
     6001 ataaggttaa gagtgcttgt tatgaatata aggttgtaaa ttcaaagcgt aaaatgtatg
     6061 gtataggata tatttcggca catagttatt ttgaacatct tctaaatgac cctgaactta
     6121 cgtctgtatt aagtgaagat taatttctta taagtagtaa gttatgtttg ataagttgat
     6181 tggaattttc tatagttttg aaggtattgg aaatttagtt ttatttaaag atttaaagat
     6241 agaggttttt agtgtttaaa attttaaagt taagttgaat aattgtatag aattatttgt
     6301 gaaagaagaa acagtgtaaa acatagtatc ttatttatgg ttttaaaatc ataggcaaga
     6361 tacttttttt atgtaactaa taccattttg agattattaa cttaagaatt ttatataatt
     6421 taacactcat atataatgat gatatttagg tagaatagat gcaattacgc ttaaataaaa
     6481 taactgtatt ttttataaaa atcaaaccct acactatact accagataaa atctacatgc
     6541 tataatttga cctgtgttta taaaaaaagg agtaatctaa taaaatagta tttcacttat
     6601 gtataactaa caactattat tacgattttc tctaaataat ttaaagaaaa ataatgcagg
     6661 aggtaaaata tgaacaatga atttataatt ttaaaaggtt gcaaagagaa caatttgaac
     6721 aatatttcct taaaaatacc aaaaagaaaa ataaccatat tcacaggggt gtcgggttct
     6781 ggaaagtcat ctattgtatt tgagacaatt gcaaaagaat cacaacgcca actaaatgaa
     6841 aggtttagta catttgtaag aagtttttta cctaagtatg gagaggttaa agctgattgt
     6901 atagaaaatt tgtcaacacc aattatcata gaccaaagta gacttggtgg aaattcaagg
     6961 tcaaccctag gtactattac agatgtaaat tcctttttaa gagctttata ttcaaggttt
     7021 ggtagtgagt atataggtaa tgctaatatg ttttctttta ataatattga tggtatgtgc
     7081 ccacactgtc atgggcttgg aaaaaagcta gttcctaata tgaaccaaat tcttgatatg
     7141 aataaatcat taaatgaagg tgctatattg ctttcagggt ttggagtggg ttcttggcat
     7201 tggaatatat tcgctgaatc aggatttttt gatattaata agaggatatg tgattattca
     7261 gaagtagaat tggaaaagtt tttgcatggt aaagcagaaa agattaagat agaaaatgct
     7321 ggtcaaacta acatgacata tgagggatta atggttaaat ttaatcgcct atacttaagt
     7381 agagagggag aaatatctga agctactaag aaaaaattga gtaaacttct tatagaagat
     7441 agatgtccta tatgtgatgg aagaagactt aatgaaagag tatataggtc tttaattaat
     7501 ggttataata tagcagattt aactagtatg caaattgatg aattggctga agttattaaa
     7561 agtatagatg agccagaagc acaaccttta attaaaggaa taatagaaaa gttaaacagt
     7621 atcatagata taggtcttgg atatttaaca ctggatagag agacatcttc tctatcagga
     7681 ggggaatctc agcgtataaa gatggttaag tatttaaata gtaatcttgt tgatttaatg
     7741 tatatttttg atgaaccaag tgtaggactt catccaaaag atgtgtataa attaaataat
     7801 cttttaaaaa aattgcgtga taaagggaat acaattatag ttgttgaaca tgacccagat
     7861 gttattaaga ttgcagacca tattgtagat gtaggaccaa aggcaggtaa gtatggtgga
     7921 gaagttgtgt atgaaggcag ttatgaaaat ctacttacaa gtggtacttt gacagggaat
     7981 gctttaagta aatttcttcc tattaaagaa agtgtaagag aacatagtgg atatttagaa
     8041 gttaaaaatt gtaataaaaa caatttaaaa aatgtatcga ttaagatacc taaaggtgta
     8101 ttgactctag ttactggagt agcaggagca ggtaaaagta cgttgattaa ggatgaattt
     8161 ttaaagcaaa atcctagtgc tgtacttata gaccaaagcc ctgtttcagc aaattcaagg
     8221 tcgagtttgg caacatatag tggaattatg aacaacataa gaagcatatt ttcaaaaaca
     8281 aatggcgtaa atgcttcttt attcagttca aattcagaag gagcttgtga taactgtaag
     8341 ggttctggaa tagtagaaat gaatcttgct tttatggaga gtataaagag tacctgcaat
     8401 gtttgtgaag gtaaaaaatt taaaaaagaa gttttagaat ataggtttca agggaagaat
     8461 ataattgaag ttcttgaaat gtctgtttta gaggcaattg agttttttaa tttaaaacag
     8521 attaaaacga aattacaatg tattgaaaaa atgggtattg gataccttac tttaggacaa
     8581 actcttgata ctttgtctgg aggagagtgt caaagattaa agcttgctag tgaattaaat
     8641 aaggaaagtt cagtgtatat attagatgaa ccaacaacag gtcttcatat ggctgatatt
     8701 gagaatttta taagtataat tgaagagatt gtagataatg gaaatacggt cataataatt
     8761 gaacacaata tagatgtaat taaaagagct gattggatta ttgagctggg acctgaagga
     8821 ggaacaaaag gcgggagagt aatatttgaa ggtattccaa aacaattatg taacaataaa
     8881 atatcattaa cagcaaaata tatagtataa taaataaaat ataataaaat tataatgata
     8941 ttttaataaa tataatgggt atataaaatg ataattaaac ttaacagtaa taaataatgt
     9001 gagaaatatt caaatttaat taaataaagt gtcagaaatt tgtttaaatt ttagtatttt
     9061 aacggttctg accttttttc ttatttttat ggagaatatc gaggatggag tctctaatag
     9121 ttactttttt gtgtctactt gcaagtttga ttaaaaaatg gctatacaca cttgtgactt
     9181 cagtcatggg aggttcagat tataatctta atatataatt tgtgaaataa tttgatgacg
     9241 atttgctgat gtataaacaa taaagattaa ctacattaaa atttaaatct tgttaagtaa
     9301 aacttataaa aggagagaga tggatgataa atgatattct aaccttatta tctgaaagaa
     9361 gtgatttttt tataaagcta tccatagaac atttgatgat ttctattatt tctataataa
     9421 ttgcgattgt tttgggtggt ataattggta ttattgtaag tgaatttcat aattcttcta
     9481 aacctatatt gggaactatc aattttctat acaccattcc atctatttct atgctaggat
     9541 ttctgatacc attttccgga attggaaata ctactgctgt tattgcactt accatttatg
     9601 cattattgcc aatggttaga aatacatata ctggaattac taatgtggat aaaaatattc
     9661 ttgaagcagc taggggaatg ggaagtacag attttcagat attgtataaa ataaaacttc
     9721 cgttagccct acctgttatt atgtctggta ttagaaatat ggtgattatg acaattgcat
     9781 tagctggtat cgcttcattt ataggtgctg gaggtctagg tgtagccata tatagaggaa
     9841 tcacgacaaa taacacaata atgacaatta ctggaagtat acttattgct ctattagctt
     9901 taatatttga ttttatttta gggctggtag aaaaattttt tcaacgaaaa agaaaaaaga
     9961 aaaataggaa aaaagttttt gccattctag gaagccttat tcttatagca actatcttta
    10021 cgtcttattt atttaatcct tcaaagactc aaactattca tattgctact aaacctatga
    10081 ctgaacaata tattcttggt gagatgttgg atattttaat tgaaaaaaac acaggtctaa
    10141 acgtggaaat tacacaaggt gtaggagggg gaacatctaa tatccagcca gctatggaga
    10201 atggagaatt tgacatttat ccagagtata caggtacagg ctggaatatg gtacttaaga
    10261 aaaatgaatt atatactgaa agtatgttta gtaaactaca gtcggaatac aaaactaaac
    10321 ttaaaatgca atggactggt atgtatggtt tttctaatag ctatgcatta gcagttagaa
    10381 aagaagttgc agataaatac aatttaaaaa atataacgga cttaaaagat gtatcttcta
    10441 aattgacatt tggagctgaa tatgactttt atgagagaga ggatggatat actccattgt
    10501 gtaatacata tggactgaac tttaaagata cagttgattt agatattgga ttaaaatacc
    10561 aagctatcaa tgaaggtaag attgacgtaa tgcctatatt tacaactgat ggtcagtata
    10621 gtgtttcaga tgttgtagtt ctaaaagatg ataaaaattt ctacccatct tatcaatgtg
    10681 gaaatgttgt tcgtttggaa gtattagagg aaaatccaga actatcaaag gtatttaaga
    10741 tggttggaga tattttgact gataaagaaa tggctaaaat gaattatgac gtagaaaaca
    10801 acaatttaga acctagagaa gttgctcgtg agtttctgaa taagaaaggt cttttataaa
    10861 ttggaggaaa tataatgaca cctattatac aatttaaaaa tattaaaaaa caatataatg
    10921 ataaaactat aatagataat cttaatttag atatagaaaa aggagagttt ttgactgtaa
    10981 ttggttcgtc tggttctggt aagacaacac tcttaaaaat gattaatggt ctgattttgc
    11041 cagatggagg aaatattttg ataaaccaaa cagatatcaa aaacgaggac ttaattaaat
    11101 taagaagaag aataggatat tgtgtacagg gaagtgttct ttttcctcat atgactgttg
    11161 aagaaaatat ttcatatgta ccaaatctcc taagtaaaaa gaataaatta gaagtaaaaa
    11221 gtgctgtaaa caagtggatg gaaatagtgg gattgccaaa tgatatgaaa gtgcgttacc
    11281 catcagaatt gtcaggtggt cagcaacaac gtgtagggat tgcaagagct ttagcttctt
    11341 ctccagaaat tttattaatg gatgaacctt ttggtgcagt tgatgagatt acacgaaagc
    11401 aattacaaaa agagataaaa gaaattcata aaaaaacagg agttacaatt atctttatta
    11461 cgcatgatat ttatgaagct ttgattttgg gaacaaagac tttggtatta aatcatggag
    11521 ttgttcaaca gtatgatact cctgagaata ttttaaatac tcctgctaac caatttgtag
    11581 accagttatt gaatataaga aaatctataa tagatgatga taatttaaaa gaataaattt
    11641 aaatttaaaa tcaaattgtt gtgataaaaa ctattgattt agagctaact tcaagttgta
    11701 tacttgaagt ggaaaagtta gattaaggta tagaagggca tttagcagtt ggctgtagac
    11761 gaggtaactg ctgaaggtgc tttcaataaa tggctagaac ctgtatctgt tgaagattat
    11821 aataaattga aataagaagc tcaatataca gagtagatag gcacttcaaa tagaagtgtc
    11881 ttttgtgtgc cataaaataa atcaaaatat tagcaaagaa tgtgaaaaac aaaataaaat
    11941 aatatgctat ctttaaaata ggagggatgc tatatggata acaatttgat taccattgaa
    12001 aaaactttag attatataga aaaacatctt ttagatgaga tgttagattt aaatgtaatt
    12061 tctaaagaga taggctattc aaaataccat ttacatagaa tgtttacatc agttgtcgga
    12121 tttagtgtac ataattatat acaaagacgt agacttactg aagcagcgag aatgctaatt
    12181 tttacagaaa aaccaattat tgagattgct ttaatctcgg ggtatgatac acaacgctct
    12241 ttttcaagag ggtttaaaaa aatgtttaaa cgtagtccta atttttttag aagacatcaa
    12301 gattttttac cactacaatt aaaatttgat attcataata gaaaaaaatt acgtggagat
    12361 atggttttag atgttaaaaa agttaattgt gcagatataa atattgttgg ctattctggt
    12421 aaagtaagaa aaggatttca tattattgga aaatgctgga gggatttgca taaaaataaa
    12481 gagatgatag aaaataaggt agatttaggt tttttaattg gagttagtga ttatagcaat
    12541 tttaaagaga ttgagaatac tcctacattt acatacattg caggtgctca agtagctact
    12601 tttggcaaat tacctaatgg aatggaaaaa ttcgtattac cgaaaagtaa ttatattgtt
    12661 tttactttta ttgggaaaaa tgaggatagt atgcaaaatg ttgtagaata tatctatcaa
    12721 gaatggttcc ctaattcaag ttgccagttt aatgaaaata atttatatga ctttgtaaaa
    12781 tatggagaaa aaactgatga gaatggtttg agtgaaattc agttttgggt tccaatcatt
    12841 taaaagaagg tattatgtat aaagtagatg gatttatata ggcaaagttt tacagcagta
    12901 tgtatttata aataatttat agtttgtagg aaagaaata
//
LOCUS       DAEOSP010000114        12867 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.114, whole genome shotgun sequence.
ACCESSION   DAEOSP010000114 DAEOSP010000000
VERSION     DAEOSP010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12867
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.114"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            234..1055
                     /locus_tag="KRH54_002404"
     CDS             234..1055
                     /locus_tag="KRH54_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBG8580059.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    1134..1345
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1432..2748
                     /gene="argH"
                     /locus_tag="KRH54_002405"
     CDS             1432..2748
                     /gene="argH"
                     /locus_tag="KRH54_002405"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBG8580060.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELQKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            2997..3335
                     /locus_tag="KRH54_002406"
     CDS             2997..3335
                     /locus_tag="KRH54_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBG8580061.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(3462..4751)
                     /locus_tag="KRH54_002407"
     CDS             complement(3462..4751)
                     /locus_tag="KRH54_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG8580062.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            4831..5535
                     /locus_tag="KRH54_002408"
     CDS             4831..5535
                     /locus_tag="KRH54_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBG8580063.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHSKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            5627..6673
                     /gene="selD"
                     /locus_tag="KRH54_002409"
     CDS             5627..6673
                     /gene="selD"
                     /locus_tag="KRH54_002409"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5675..5677,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBG8580064.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            6691..8103
                     /locus_tag="KRH54_002410"
     CDS             6691..8103
                     /locus_tag="KRH54_002410"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBG8580065.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            8217..10100
                     /gene="selB"
                     /locus_tag="KRH54_002411"
     CDS             8217..10100
                     /gene="selB"
                     /locus_tag="KRH54_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBG8580066.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            10205..>12867
                     /locus_tag="KRH54_002412"
     CDS             10205..>12867
                     /locus_tag="KRH54_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBG8580067.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIVSGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSS"
BASE COUNT         5248 a         1311 c         2293 g         4015 t
ORIGIN      
        1 tatatatcat atattgtaaa atttctagca ttataagact ataaattgaa aatattcaga
       61 aaaatactac aagaaacgat ttattttgct cttaaaataa gttaaatgaa tgttattaat
      121 tttgtaaatt gaaatagttt aagtataaag ttatgatata atctaataag ggtaattctt
      181 tagattatat taaggaaata taggtataca ataaaaagtt atggagttgg attatgggat
      241 ataaattaat tgtaacagat atggatggaa ctttattagg caataatcat aaagttacag
      301 atgaaaataa aactgcacta caaaaagtta taaaaagcgg tattaatgta actttagcaa
      361 caggaagagc cttcgattca gctaaatgca atgtagattt tcttaaagag gacatgccaa
      421 taatagcttg taatggttca ttaataagag agcaaaatgg aaacataata tattcaaata
      481 agattgatac aagaacatgt ttaaacattt tagatgtatt agataaatac gatatttact
      541 accaatgtaa tagtatagat tctatgcttt cgaaaaaaat agaaggacgt gaagatagat
      601 taagtgtatt tttaggctct gaaacagaag taattgtaaa agatgatttg agagaagaaa
      661 tctttaaaaa agatatacta aaatttgtaa tcatagaaga aaaaaatcca tctattttgg
      721 atgaaataag aaaagaattg agaaaagttc aaggaataaa gataacttct tcatggccaa
      781 acaatattga agtcatgaat gaaggtgttg ataaaggtaa tgcagttaaa attttagctg
      841 aaaaaatgaa tatagacaga gaagatataa ttgcatttgg agacaattac aatgacatag
      901 aaatgataaa gtttgctggt cttggggtag ctatgggaaa tgcagaagaa ttgattaaac
      961 aagaagctga ttatgttaca gatacaaatc aagacagtgg agttgcaaag gcaatttata
     1021 aattcctaga gttaaaagaa tcacttagct cttaaaacag tatataatag gaaaaaatag
     1081 gttgactatt ttgtagatag taattataat acaaatagat aaaacataaa aatttagatt
     1141 cagtgaaaaa gagagtagat atagagaaaa tctcagcgaa ttgggggatg gtgtgagcct
     1201 aatgataaca ctttatattg aaaagaactt tggagaatac tacttgaaat tcagtatggt
     1261 agtaggtaat ctcgccttaa gagaatagag tgggtaaaca ttgtttacca actagagtgg
     1321 taacacggaa tatttcgtct cttggtcttt ttgaccaaga gattttttag tttcattgag
     1381 ttacaagact tataattaaa atacaaaaaa ttaaaatatg aggtgaaagt tatgaaatta
     1441 tggggcggac gctttagaaa agcggaaaat caacttatgg aagaatttaa taaatctttt
     1501 ggctatgatt gtgtactgta caaaaaagat atcgaaggaa gtgtagcaca tgttcatatg
     1561 caagtaaaat gtggactttt aacagaagaa gaaggaaagt ctataacaga aggtttaaaa
     1621 gggatacttg aagatgtaga aaatggaaag ttagtattgg atggagagta tgaagatata
     1681 catagtttta ctgagataaa tttaattcaa agaattggag atgttggcaa gaagcttcat
     1741 acagcacgta gtagaaatga ccaagttgca gttgatatga gattgtatgc taaagaaaaa
     1801 gcaaatgatt tggtgggttt aatatctgaa tttaaagcta ctataaaaga tgttgctgac
     1861 aaaaacccag tgatgatgcc gggctatact catcttcaaa gggcacaagt agtgacattt
     1921 aagcatcact taatggcata ttatagtatg tttgatagag atgagaaaag aataaaaaat
     1981 gctatagaaa tattagatga atcaccttta ggctgtggag ctttagcagg aactacgcat
     2041 gatatagata gaagtataac ttgtgaacaa ttaggattta aaaaagtagt agataatttt
     2101 atggatggag taagtgatag agattattta ctagagctaa tgagtgattt ttcaattata
     2161 atgatgcact taagtagatt aagtgaagag ttgatacttt ggagttctca agaatttgga
     2221 tttgttgaga tagatgattt atatacaact ggaagtagta taatgcctca gaagaaaaat
     2281 ccagatggtg cagagcttat aagaggtaag actggtagag tatatggaaa cttatttggt
     2341 ctttttacag taatgaaagg aataccatta gcatataata aagacatgca agaagataaa
     2401 gaaggttttt tcgatagtgt acatactctt gaaatgtgta tacaaataat ggatagaatg
     2461 atagccacac taaaggtaaa tgaggataaa atgaaacaag cagtaaaaaa tggtttttta
     2521 aatgcaacag aagttgcgga ttatttggtt aaaaataatg ttgcttttag agatgcacat
     2581 ggtatagtag gaagtatcgt tatatattgt gaagataata aaaaagcaat agaagattta
     2641 acattagaag aattacaaaa gttttcagat gcttttaagg aagatattta tgattttatc
     2701 gactatgaga gtatattaaa taagggaata aaaaagaatt taaaataatg tatttatttt
     2761 taaaagctgt aaatttgaat gttatcattt aaatttacag ctttttattg tgtaagattt
     2821 tattatataa aagtaagaaa cttaaaagga gtagtattta aaataaatac aaataaaaaa
     2881 ttattattaa aatgataatt aatatcaata agtattgaca aaattatttc taaatactat
     2941 tatatataat ataaataata atgaaaatca atatcaaata aatgggagga aatctaatga
     3001 acttattagt gattggtggt catgaaagaa tggaaaaaga ttatatggtt atggccaaga
     3061 aaaaaggtta taagacaaag gtatatacta ccatgtcatc taaattaaat aattctatag
     3121 gaagaccaga tgcagttgtc atacttacat caactgtttc acataagatg tctagaacgg
     3181 ttgagtctca agctaaaaaa caagatatat taatcgtaag gcataaaaat agtagtaagg
     3241 tagcatttaa tgaatgtcta gatatggttg atgaatgtct gggaaattgt tcaaaatgta
     3301 agcttaaaga caaacaaaaa caaaaaaata tataggtata aaaaatacat aaacataaaa
     3361 ttaaaagtac tgagataata gttataatta ttatctcagt acttttagat ttataaggat
     3421 aagtatagta taaatttttt attataattt ccaactactt gttacttttt catatttttt
     3481 ttacttctag ctttttttct ttttttcttc tttatatttg aagctattat aaataaaact
     3541 aaaagcacta gaatacctgc aataatctta acaaaagtca ttaaaacatt attttcagtc
     3601 aaaaatggaa gactgctatc aagattattt tttgcaatta agtctatact gttttctagt
     3661 ttaccattat tatatacatt taaagtacca accttagccc ctttttttat aggcaaatca
     3721 attttattta actctgtttt ggtatcataa ttgccttgag attcatcttt ttctaagaat
     3781 atcctataac tattttttgg ttcataaatc aattcatgtt gttttgtaaa caatactttt
     3841 ttagtatctg tgtatttttc tttatcaaca atagtagcac acttaaagtt atcaaatcca
     3901 taatcaatca gaatacgtga gtctaaatat aaatcagcat tagttgaatt aaatacagca
     3961 acgataactc ttctgccatc ttttacagca ctcgataaaa gacatttacc agcagcatca
     4021 gtatatccag tttttatacc atcaactata tcatatttta tatttatagg ttgacctttg
     4081 tatgtaatct tatcatgtga tgtcaaaaac ttattagtgt ttacaaagta tctctcataa
     4141 ggatatgctt ttgtagcttc aaatttaagt gacttagttt ttacaatttg tctaaaagta
     4201 tcattattca tagcttctct agctatgaga gccatatcat gagctgttgt gtagtgattt
     4261 gggtctggta atccatgagg attattaaaa tgagtattag tagctccaat tttttttgcc
     4321 tcagagttca taaggtcaat aaatttttgt acattgccac caccaacata tctagctaat
     4381 acaaaagcag catcattagc agaatgaacc aaaagagcat ctagtaactg ctttacagta
     4441 aaagattccc cattttttag atacatacta gaaccatcta tttgaggtaa atctttaatt
     4501 tctattactt gatttaaatc tttaacctcc tttagaacta aacaagctgt ccatacttta
     4561 gttgttgaag ctggataaag cttagaattt ccatccttat tatatagtat tttgccagac
     4621 tcatagtcca ttaaaactgc atgtttagca actatagctg gttcattatc tgcaaaagag
     4681 atattagagc ttcctaaaaa tgaagtaaat atcaataaac agattaataa aagtgataaa
     4741 tttcttttca ttgtgagcct ccaaatttta aattcatctt ttagtatagc atatatttgt
     4801 caataattaa aagaaaggag tgattaatat atgaaaaaaa aggtaaaatt tatttcgttt
     4861 tttgttttat ttattttaat aatatcatca ataatcgttt ataaatcgct tattttcaaa
     4921 gatgatattt atgttgtaag taaaaaaaat ttacctaatg aaaaaacaca tgttgtagag
     4981 aatgaaatta aaggtgaaga taataatcac agtaaagtaa ctaaagacat agataaaaat
     5041 tttcaaaata ataaaataac tatatacata agtggagcag taaatagacc tggaattgta
     5101 actatagagt cagacaagag actttatgat gctgtagaat tactaggagg aacgacaaaa
     5161 gaagcagatt taaatggagt caatctatca gtaagattag aagatgaaca acattacata
     5221 atacctaaaa taggtgaagc aacatctgtt acaagtaatg atgattccaa taaaccaaat
     5281 cagaagaatg agtcaagagg tgagcctaaa aataaatcca ataaaaatat atctaacgaa
     5341 tcaaaagtaa atataaatgt agcgactata gaagagttgg attctctacc tggggttgga
     5401 gaagctacag ccaataaaat tttacaacac agagaagaaa atggacagtt tagttcaatt
     5461 gaggaaataa aaaatgtaaa tggtattgga gataaaaagt atgaaaatat aaaagattta
     5521 atttgtgtag actaaaatat aatacaaaac ttacatatag atatgttatt ataaatatgt
     5581 atgtatatgt gtgtacatgt ggaaatttag taaaggaggc acttttatga aaaaagaaca
     5641 aaaaaaactt acagagatga caacagcagg tggttgagcc gctaaaatag ggccagaggt
     5701 tctggcatca gttttatctc aattgcctaa aaatgataat atagaaaatt tattagtagg
     5761 actagataca gcagatgatg ctgcagttta taaattaaat gatgatatgg cacttatcca
     5821 aactttagac ttctttacac caatggtaga tgacccatac gtatttggtc aaattgctgc
     5881 atcaaattca ctatcagatg tatatgctat gggaggcaaa cctttggttg ctatgaatat
     5941 tgtttgtttt ccatcatgcc atgatatgga tgtacttgca gaaatattaa aaggtgggtt
     6001 tgataaagtg aaagaaagtg gagcactttt ggttggaggg catactgtag atgacaaaga
     6061 gcctaaatat ggtctttcag tttctgggat tgtaagtcca aataaagttt tatcaaatgc
     6121 tactgcaaaa cctggtgata agttaattat aacaaaacca attggagtgg gtgtattaaa
     6181 tacagccatg aaagaaggta tggtagaaca acatatagca gataaagtaa tagaaataat
     6241 gatacattta aataaatatg cagcaatgag ttttgagaaa tttgatgtaa attcggttac
     6301 agatataact ggttttggtc tacttggtca tactttagaa atggccaaag cttctgaagt
     6361 aagtattgaa attgaatcaa aacatgtacc aattattgaa ggtgcaatag aaatggcaca
     6421 aatgggaatt atacctgctg gtatgtataa aaatatgcat tatgtatcta aagatgtaga
     6481 agttgtagga aatatagaag ttgcagttca agatatttta tacgacccac aaacatcagg
     6541 agggttgtta atttcagtaa aagaagaact tgcagaggag cttgtaaaag atatgaaatt
     6601 aaatggagca atagaagcta aaattatagg ttctgtagtt ccaaaagggg aaaaatatat
     6661 tacagtttta taaaatatta tagagaaaga gtgattgttt tgagcaaaag agaattgttt
     6721 gcaatgttgc cttcagttga tgaagtatta agtgataata gaattgtaga aataataaat
     6781 gaatatccta gaagtttagt tttagaatct gtgagagaag taatagattt aaaaagacaa
     6841 tttatattaa gattaaaaga agatgcatca aactctgtaa caatagagtt tggtgaaata
     6901 atagaatctg caatagagag agttaagtta aattactcat tatctttaaa aaaagttata
     6961 aatgcaactg gtacagttat tcatacaaat ttaggaaggt ctttacttag tgaagatata
     7021 aaagatgaat tatggtgtgc tgcttcaagg tattcaaact tagaatacga tttggataat
     7081 ggagaaagag gttcaagata ctctcattta actagtacaa taaaaagact tactggagca
     7141 gaagatgtac tagttgtaaa taataatgca gcggcagtac ttcttgtact tagcacaatg
     7201 gctaaaggag gagaagcaat tgtatctcgt ggagaattag ttgaagtagg tggttcattt
     7261 aggattccaa gtataatggc tcttagtggt gcagaattag ttgaagttgg ttcaactaat
     7321 aaaactcatt tgaaagatta caaggaggcc attacagaag atacaaatgt attgatgaag
     7381 gttcatacaa gtaattatag aataatggga ttcacagaga gtgtttctat agaagaattg
     7441 gttaatttag gtaaaaaata taaactacct gtaatagaag acttaggaag tggagttttt
     7501 atagatttat caaaatatgg tttatcttat gaaccaactg tattggattc tattcgacaa
     7561 ggtgcagatg tagtaacttt tagtggagat aagatgcttg gaggtccaca agcaggtatt
     7621 atagttggga aaaaagagta tatagaaaaa atgaagaaaa atcaacttac tagagcactt
     7681 agagttgata agcttacaat atgtgctctt gaagcaacat tgagaatgta tttagatgag
     7741 acaaaagcta tagaaaatat acctacttta aaaatgttga catataaaat tgaagaatta
     7801 gaagtaaaag caaataaact atttgaaaaa ataactgctt taaacttaaa tgcaaacata
     7861 aatatagaag atggattttc acaggtggga ggagggtcta tgcctcttga aactataagt
     7921 acaaaggtaa tatcaataac tcctgaacat atgaatgttt catctttaga aaaaaaacta
     7981 agacttagtg aagcacatat aatagccaga gtgtatgata ataaatatgt actagatgta
     8041 agaactatat ttgatgatga atttgatgta atagttgagg aattgagaaa agcatttaat
     8101 taaataaatt tgagttaata aatattaaat ttagaagtat aagtatgaag ttttgattaa
     8161 aaaatagcta cacacacttg tgactttagt catagcaggt ttagaggggg caaaaaatga
     8221 aaaatgtagt aattggaaca gcaggtcata ttgaccacgg aaaaactact cttataaaag
     8281 cattgactgg aagagagact gatacgttag atgaagaaaa aaagagaggt atttcaataa
     8341 acttaggatt tacttatttt gatttaccaa acaatagaag agctggaatt gttgatgtcc
     8401 caggacacga aaaatttata aaaaacatgt tagctggagc atcaggaatt gatatagttt
     8461 tacttgttgt agcagcagat gaaggggtta tgccacagac agttgagcat ttagatattc
     8521 taagcttttt aaatatcaaa aatggaatag tggttctaac taagtctgat actgtagatg
     8581 atgaatttag agagctagta aaagaagata tttgtgaaaa aataaaagga acttttcttg
     8641 aagatgctga aataattgaa gttgattcta tatctaaaag aggtatagac actcttattg
     8701 aaaaaataga taaaatgagt gatgatatag aagataaaaa tgaaaactct ccagcaagac
     8761 ttaatataga cagagtattt tcaataaaag ggtttggtac tgtaataaca ggcactttaa
     8821 tagaaggaaa gataagtata gaagatgatt tggttatata tccacaggta ttaaaaacaa
     8881 aaataagaag tatacaagtt catggagaaa ataaagatac agcttatgca ggtcaaagaa
     8941 cagcaatcaa tatatcaaat gtaaaagtgg atgatataaa aagaggagat gttttagctg
     9001 caccaaactc attggaggaa tcaatgatgt tagatgtaaa attatcattg gtaaatcatt
     9061 tagataaagg attaaagcac tgggatagat taagattgta tcatggaact agagagatac
     9121 tttgtagagc agttccacta gataaagaat taattgaaag tggagaaagt ggctatgttc
     9181 agttgagact tgaagaaagc atagtatcta agaaaggtga tacgtttgta gtgagacgtt
     9241 attctccaat ggaaactata ggtggaggtg taataattga cccatctcct aaaaaacata
     9301 agaaatttga tgaaaaagtt atagaagcat taaaaataaa agaaaaaggc gaactaaaag
     9361 atataataga agagtactta aaaaggaatt taaaaaatta tccaaatata aaagaaataa
     9421 tgagctatag tggtgctcat gaagaagatg taaaaagagc gcttgaaaca cttatatcag
     9481 aagataaagt atttattata ggaaatatgt atatgcatat aaatcaatac aataaattaa
     9541 aagaaaatac aataaagctt ttaagtgagt accataagaa gtataggctt agaaaaggga
     9601 tactaaaaga agaagtaagg tctaagatag agagtaattt taagactagg gagatggata
     9661 ttttattaga aaaattgtct acagaaaatg ctataaaaat agaaaataat atagtatctc
     9721 tattagattt tgaggttatt ttaaatgata agcaaaaaga aatagcaaaa aaaatagaaa
     9781 aaagattaaa atcatgtgga gtttcttcta tactgacaat agatgaagtt tctgaaggaa
     9841 atcataacta tgctgaagta ttggagtcaa tgataggtaa taaagtagag aaattagatg
     9901 atttgtatat aatggacaaa gatatttatg aaaatgcaaa gaacattttg ataaattata
     9961 taaaagaaaa taaagaaata acattaggag aatacagaga tttgattgat tctagcagaa
    10021 agaattgcat gattatattg gaaaacttcg atagaaacaa aataactaaa agagtagaaa
    10081 acaaaagaat tttgttttag tagaaaatgt ctgtttttgc tttaaggtat aaagaaggaa
    10141 ttataatgtc gagtatcgta taattaatta gtataaaata ttaattaaga ggaagtgtaa
    10201 agtattgaag aatataatac ctgatgtaaa accagatata aaagaaagag aagacatgag
    10261 ccttttattt ataataatgg gctcagtact tttattattt attataaaag atatagagaa
    10321 atatgaaact ttattacatg ccatatatac gatattttta gtagctacag gagtaattat
    10381 atttaatact acaaaattta aagtagattc attttctaca tttttaggat taatttttat
    10441 agctactgga gtattggagt gtatatattt atttaattcc ttggggatta aaactaagtc
    10501 tataatggag attaacatca caatatcagc aataacagat ttatttccta tactgggtgt
    10561 atacttaagt tttaaatttg ttaaggataa caaacaaatt tattcaagtg taattttatt
    10621 tatagcaact gctacattaa caataacatc tttattcata atatcaggaa tttgtgacta
    10681 tctgggaaat ggtaaatcac aatatgcttt aggagttata atatctatat tcataataat
    10741 tatttctatc gttagtggaa ttgaattaaa ttcaagttca aataaaggta aatgggaata
    10801 tgatgaaaaa aagattttta acagaataat agtaataata atcttatcaa gagttccaaa
    10861 tctattgcat atagttattg ataatagaaa tgtagagagg atattatcac aaataataat
    10921 aaatgtagcc ttatattatt tatataatta catagtatct aagaatatta agaaaacagc
    10981 actggaattg catgatacga atgaagaatt aacaaaaaaa acggaaagtt taaaagaaaa
    11041 gaataaaaaa ttaatctatg agacagaaaa gatagaggaa cttaagggaa tacttatgaa
    11101 aagagaatct aggctacaat ctacattaga tgtagcagta aatagcatag tagtatttag
    11161 caaagatgga gaaattacat atgcaaataa gagctttaga aatacttttg gagactataa
    11221 agaacaagat aaattgactg ataaaataaa aaacttcaat gatttgatag aaagcattca
    11281 ttatgtgttt gttcatgaga agaatgcaga gaaacttatt agtacaagcg ataataaagt
    11341 ttatcaagct atatttgcac ctttaattat tgcttcacaa acagaaggag tattgtgtgt
    11401 actagaagat aagactaaga aaaaggagta cgaacgcaaa cttattgaag caaataagag
    11461 aagtaaagat tttttagaaa gtattggaga tggcatagta gtgcttgaag gtaataacaa
    11521 aatatatgtt aataatgcat gtagggaaat ttttaaaaat aatttagaga gtattgattt
    11581 ttcattagta tgtaaaagtg aagaaagtat agaaaaaaga tatgtaatag atggtgaagt
    11641 aaagtatgta gagatgagtt tttctcagta tactaatgct ggtactaata aaactattat
    11701 tgtaatacgt gatacaacct ctcgaaaaat atctcaaata aaacttgagg aaagtcaatc
    11761 atcatattca agatttatag atatattacc tgatggcata tgcttattaa aagaagattt
    11821 aagtataaac tatgcaaata agtcgttttt agatatgcta gcttttacaa acatagatga
    11881 tataaaagat tctaatatta aactattaat gaatgcgaat actgaagaaa aaatgaaatt
    11941 tacggataat atggctaaag ttttaaatga aaatagaagt atacttttac tggaatatga
    12001 attgataaat agttatgatg atatagtaga agtagaatta agtgcattac cttttgctat
    12061 atacaatact agatacataa tgcttattat aaaagattta gtacataaga aatattccga
    12121 gcaagcagaa aaagaactgc ttgaaagatt taaaacagat aaaatcaaaa cggagttttt
    12181 tgcaaatatg tctcatgaac taaaaactcc actaaatgtt atatctagtt caaatcaact
    12241 ggtagattct ttctatagaa atgaaaagat aaaagattac aataacaata ttaaatctca
    12301 tgtggattta gttagacaaa gttcttatag gcttcaaaga ttaataaata acattataga
    12361 tttaacaaag atggagtctg ggttctatac actaaaactt gcgaaataca atattgtatc
    12421 tgtaatagaa gacttattta tgaatataga agagtatgca cttagaaaag acataaaaat
    12481 tctttttgat acagaccttg aagaaattaa tgtttatata gacaaggttg aaattgagag
    12541 aattatgttg aatctattgt caaactgtat taagtttact gataatggag gctggatata
    12601 tgtaagtatt cattataaaa tagataaagt aatcataagt gtaaaagaca ctggagttgg
    12661 aataccacaa gataaattag agttgatttt tgaagaattt tcacaagttg ataagacttt
    12721 atccagaaat actgaaggaa gtggaatagg gcttgctata gttaagaatc tagtaagtct
    12781 tcatggtgga gatatagaag tagtaagtga agtgaataaa ggcacagagt tcttaatttc
    12841 acttccaatg aagggattta gtagtga
//
LOCUS       DAEOSP010000115        12782 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.116, whole genome shotgun sequence.
ACCESSION   DAEOSP010000115 DAEOSP010000000
VERSION     DAEOSP010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.116"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(161..3496)
                     /locus_tag="KRH54_002413"
     CDS             complement(161..3496)
                     /locus_tag="KRH54_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBG8580068.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTANYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIWEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLTSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGQEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(3545..3973)
                     /locus_tag="KRH54_002414"
     CDS             complement(3545..3973)
                     /locus_tag="KRH54_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580069.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(3963..4343)
                     /locus_tag="KRH54_002415"
     CDS             complement(3963..4343)
                     /locus_tag="KRH54_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBG8580070.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(4376..4684)
                     /locus_tag="KRH54_002416"
     CDS             complement(4376..4684)
                     /locus_tag="KRH54_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580071.1"
                     /translation="MNQIELDLYHNMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(4717..5901)
                     /locus_tag="KRH54_002417"
     CDS             complement(4717..5901)
                     /locus_tag="KRH54_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBG8580072.1"
                     /translation="MVDEAKILDVVSISGENVKKFDGERSYLSTGNLDFNKISNLEIV
                     TYENKPSRANQTVNIGEVIFAKMKDTKKTLVINKTNKNIIVSTGFYVLKPSKEILPQY
                     LYHYLNSSYFLNQKNRLSKGATQSALNNEGLANIKIRMYNLKVQEKVVRVLDKAQELI
                     DKRKEQIEVLDELVKSRFIEMFGTPSKNEKNWEISEIGKYLDVLTDYHSNGSYETLRD
                     NVTLLDTKGYALMVRTTDLENNNFEKGVKYIDEHAYNYLEKSKVFGGEVIINKIGSAG
                     KVYLMPFLNKPVSLAMNQFMLRFNEDKVNHIFLYNLLLTSYMESKIKEKVRGAVTKTI
                     TKDAVRKINIIVPPIRLQNQFANFVKQVNSLKFEMETSLKELEDNFNSLMQKAFKGEL
                     FN"
     gene            complement(5905..7368)
                     /locus_tag="KRH54_002418"
     CDS             complement(5905..7368)
                     /locus_tag="KRH54_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBG8580073.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFNDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIMGRVKALEKEIVDGLEELERMLEV"
     gene            complement(7878..8747)
                     /gene="gatY"
                     /locus_tag="KRH54_002419"
     CDS             complement(7878..8747)
                     /gene="gatY"
                     /locus_tag="KRH54_002419"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG8580074.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKNVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIETVKC"
     gene            complement(8767..10683)
                     /locus_tag="KRH54_002420"
     CDS             complement(8767..10683)
                     /locus_tag="KRH54_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBG8580075.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(10686..11606)
                     /locus_tag="KRH54_002421"
     CDS             complement(10686..11606)
                     /locus_tag="KRH54_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBG8580076.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLEDISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(11708..12460)
                     /locus_tag="KRH54_002422"
     CDS             complement(11708..12460)
                     /locus_tag="KRH54_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG8580077.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
BASE COUNT         3958 a         2446 c         1290 g         5088 t
ORIGIN      
        1 atttatcaat taaaaagaga tattgcataa ttattcaata cctctctttg tatatttaca
       61 ttatccatat ctagttatct atttcaaccc aatattagac tacctactaa taaatttatt
      121 ctctacatca ttaacaataa ttttgcacta aataacatca ttagcctatc tctgatgcat
      181 tttctttaat tttatttata gttttaatca atttatttct ctcttcaata tgattttcaa
      241 ataaatcaat tatacttcca acagttctaa atgggtcttc catcaacact ttgttatcct
      301 ctataaatcc attcttgact acataatcga ctataagttt aacaaaatgt atttgcttag
      361 tattaaagct cttattattt aaaaactctg aaaaagcttc atttgcagct tctctatcca
      421 atcccactag tcttctcact aattggctta ctggagtatc tccaaactcc ttcttataat
      481 catcacaatt acctaactgt tttaagagta tttcctccaa cgtttcaaca tcttgctttg
      541 ttatcttttt attatttctt aatttaaata ttgctaattc atctttatgg ctatttaaat
      601 aatactctac cttcttttta taattcttaa gattgtttgc attatataca gaatcatttc
      661 tatcttctgc tacaatcata tcttcaaaac ttgtataata tatcttttga ctagactttt
      721 caagatactt aataagctct cttaatgata tacgtacttc ttctacctca aatatatctg
      781 aatcttccca aaaatcctct gtcattgctt tttcaatagt atatttttgt tgctgaatct
      841 gtggtatagt cccaagtttt gaaagttttt ctgctgtagc cattacatgc cttatacctt
      901 tacttgcatt attattttgt aagtatgcaa gttgtatatt atacataact acatcaaatc
      961 ttttagccaa ttcatcatca ctatatgaag ttatgagtgg tgatatatat tctcttatct
     1021 cattttgtgc tactgctcca atattgctcc aaacattttc atttttatac ttatgtacat
     1081 aacttaaatt catcctaact atataactat cctcatttaa agcttttaca gaagttataa
     1141 gttcttctaa caattctttt ctaagttctg catactcacc ttctatatat ttaatatctt
     1201 gaagttcttt aaccatactt acctttatat taaataattt ttcagtaagt gtttctgctt
     1261 tatttccctc aaatccttta ggattaaatt taaaaaattc aaaattatta caaaaatcaa
     1321 atattaagaa tctctcctta tccatatcaa cacctaataa atctggacat aatctagttc
     1381 ctctacctat catttgccaa aacttagtct tacttctcac tttcttgaaa aaaacaagat
     1441 ttagtatttc aggtatgtca attcctgtat caagcatgtc tactgaaact gctatttgag
     1501 gtaatttatt cttatcagaa aaatcatcta ttatagaatc tacataattt atactatagt
     1561 caactgcttt tacaaagttc ccaccatact ttggatacag cttattaaat ctttcaacta
     1621 ttgccctagc atgctttgta ttctttgcaa aaattatagt cttaccaatc ttttcttgtc
     1681 cttctatttt caacccctct gtcattagct tatttaaaac taaatctatt gtgtcagaat
     1741 taaatagcca ctcatttact gcattattcc ctatttcgtc tccaatactc tcatcatcat
     1801 taaatgtatt ttcatattct tccttctctt catcagaaag ctcatcatac ttaattccat
     1861 cttccataat cttagtctta aactcaatac tagtataatc tactaaatat ccatcctcga
     1921 ctgccttatt atactcataa gcatatgtag gtactccatt ttccatgtca aaaactgaat
     1981 aagtattttt atctatctca tcctttggtg tagcagtaag acccattaag taagaatcaa
     2041 aataatcaaa gattgactta tactttttat aaatacttct atgcgattca tctattataa
     2101 ttaaatcaaa atgaccaggt gtaaacaatc tctttccatc cttagacttc gtatcatcaa
     2161 ttgcattcat cattgttgga tatgtagaaa atatcatcct agcatcttct gggctatctt
     2221 tgctatccaa taaattacat aacgccaagt taggtaatag cttagtaaaa ctttttttag
     2281 cttgctttac caatgctttt ctatctgcta gaaataatat atttttaatc caattatgtc
     2341 ttgaaagtac atcaactata gatatagcaa ttcttgtttt tcctgtacca gtagcacata
     2401 ccaaaagagt tttgcgttgc ttattttcaa gagcctcaca tacagcccta atagcttctt
     2461 tttgataata tctattggat atttcatcat ttattgttac agaagttaaa gacttcttac
     2521 tctttcttct atctatcata agttgtaatt ctgatttttt ataaaatcca taaactttcc
     2581 tatctgaata gtcatcccaa atatacatat caaaaccatt agtcaaaaat attaccggtc
     2641 tttgactata tctcttttcc aaacaatctg catataattt agcttgttgt tgaccaattt
     2701 tcgggtcttt actcgttctt ttcgcctcaa ctactgctaa tggcttacca ttttcaccat
     2761 aaagaacata atctacatat cctacaccac tttcattagg cataccttgt acttctattt
     2821 cttcccatat atcttttcca aattcccaac ctgcaagctt taattctata tttatatatc
     2881 tttttcttgt ctcaaattcg cttacttcgt ctatcttgaa atcatagttt gcagtattaa
     2941 cttttctctt ttcagttagt gactttctta attcctcatt ttcttttatt acttcttcaa
     3001 gctttttatc ttttgaactt aatctctcat aaagattttt aagttcgtct ggtctcgtcc
     3061 ttttctcttc cccatgaagt aataaatctt cattaaagtc ttctgctgta tattcatctg
     3121 aataacaata gtctatccaa ctaacaaatt gatgcaaatt gttaagtgaa agtattgctt
     3181 ctcctctctc tatgttcgca ctagtatgta cagataaatt acctaattta actatatatt
     3241 ttaataaagg taacatatca tagtctataa gttctataaa actattatta tgaatcaacg
     3301 tactgagatt atcttgataa ggcaatacta aatcactatc attagcatat agccatttta
     3361 ctgcaagctc taaagctctt cttgttatag ttgcacaagt agatggacta actagtatac
     3421 ttttttctgc ttctaagcaa gcagtattaa aactattgaa atttttcttc ttttgtaaaa
     3481 aatcaaaatt acccaagctt ttatccccct tttgtccctt agttaaaata aatctctcta
     3541 aaaactattt cttacagctg tttcttatac taacaaatac ttcttcattt tctgtaaaca
     3601 attcgtacat atcttcaaac tgtggtatat catatccatg agaatcatca tttctcaatt
     3661 ttctaagtgt ttctataact atagaagaat ttttaggtaa taaattgtga tactcacaag
     3721 ctaaaaggta gtcataaaag tttttagatt ttatgtttaa atcaattgat ttataataca
     3781 acattgcttt tgttaattct agaaatgata ccatatagct tttaaagcct tcttttatgt
     3841 atctagccca taactcaggc atttcttcct tatcatataa gattttgaag ttttgcaggt
     3901 catacaagca agaaccaaaa tccctattta cttcttctat tgagttccaa gataatttgt
     3961 ttctaattac catacatgtc cctccaatct aagatatcta ttacatcact gttttcaaaa
     4021 tagaatttat ttatataatc cttaaaatct ttagattcat aaataacttc accttctaaa
     4081 atagcttttt taagtgctat tgtataatta tctgtaacaa tttttaggtc tacatcaatg
     4141 tctaatatat ttcttagagc ttctgtctta gttgcaagtt ctgtaattgg tacttttttg
     4201 ataggaatcc aagctatgtc tatatcactt tctgatgtat gtttatctgt tccataacta
     4261 ccaaatatta tgattgctag agtattttct tttggaaata catttattat tgagttttta
     4321 atctcatttg gtatatttct catttaatca cctcttgttt attaggtaat attacttaat
     4381 taaatttata tccataatct tttaatctat ttatacatat ttctctttct tcatcagtaa
     4441 acaactcttt atacttttct tttaatacta atacttctaa tgataaatct aatctcttac
     4501 actcccatag tcttgtaaat ccttcagtag ctccttcttt attgattaat tctttagcag
     4561 ctaataaggc tcctttgtta tttatcagtt gtaaaaatct tgttggctta tagttacatt
     4621 gtaaatcagc ttctttatag atatttacca tgttatggta taaatctagc tctatttgat
     4681 tcatagattt tattccccca ttttaataat tgtattttag ttgaataact ctcctttaaa
     4741 agctttttgc attaatgagt taaagttgtc ttctagttct tttaagctag tttccatttc
     4801 aaacttcaaa gagtttactt gtttaacgaa gttggcaaat tgattttgaa gtctaatagg
     4861 aggtacaata atatttattt ttctaacagc atctttagtt atcgtttttg ttacagctcc
     4921 tcttactttc tcttttattt tactttccat ataggatgtt agtaaaaggt tatataagaa
     4981 tatatgattc actttatctt cattaaatct aagcataaat tgattcatag ctaaagatac
     5041 tggtttattt aaaaatggca ttaaataaac tttacctgca ctacctatct tatttattat
     5101 aacctcacca ccaaacacct ttgatttttc aagataatta tatgcatgtt catcgatata
     5161 cttaacacct ttttcaaagt tattattttc taaatctgta gttcttacca ttaaggcata
     5221 accttttgta tctaaaagag ttacattatc tcttaaggtt tcataactac catttgaatg
     5281 ataatcagtt aaaacatcta aatatttccc tatttctgaa atttcccagt ttttttcatt
     5341 ctttgatgga gtaccaaaca tctcaataaa tcttgattta actaattcat ccaatacttc
     5401 tatttgctct tttcttttat ctattaactc ttgagccttg tctaaaacac gcactacctt
     5461 ttcttgcact tttaaattat acatgcgtat ttttatatta gctaatcctt cattatttaa
     5521 agctgattgt gtagcacctt tacttaacct atttttttga tttagaaaat aactactatt
     5581 taagtaatgg tataaatatt gaggcaatat ttcttttgat ggttttaaaa cataaaatcc
     5641 agtcgatact attatatttt tattagtttt attaataact aatgttttct ttgtgtcctt
     5701 catttttgca aatataactt ctcctatatt tacagtttga tttgctctag aaggtttatt
     5761 ttcataagta actatttcta gattgcttat cttgttaaaa tctaagtttc cagtactaag
     5821 atatgagcgt tctccatcaa acttttttac attttccccg cttatactaa caacatctaa
     5881 aatttttgct tcatcaacca tactctacac ctctaacatt ctttctagtt cctctaatcc
     5941 atccactatc tctttctcta acgccttaac cctacccata ataacactag gagcatcata
     6001 aactacttct tcatacacta cctctttata cttattaata gataaatcat acccattctc
     6061 tcttatctct tccacatcaa caaaaaaact ttgctctgtt ctttttctat ctacttcacc
     6121 atcaagatta tcaaatctct taataatatc ctctatatca ctatccttta tctcatttct
     6181 cttatcatcc aaagaataac catcagcttt catatcatag aaccatacct tatcagttcc
     6241 acctgttcct gttttagtaa atatcattat agctgtagat actcctgcat aaggcttaaa
     6301 cactccactt ggcattgata ttatagcttc caatttatga ttatcaacta tttcttttct
     6361 tatcgattta tgtccacctg ttgaaccaaa caatactcca tctggcacta tacatgcgca
     6421 tcttccacca atttttaaaa ctcttaaaaa aagtgccaag aataataact ctgttttctt
     6481 tgtcttagtc acttttaata aatctgctga cactgcttca taatctaaac ttcccttaaa
     6541 aggaggattt gctaacacca atgtaaactt atctttatct gtattaacct ccgataaaga
     6601 atccttatac tctatattag gattgtcaac gccatgcaac atcatattca tagcacctat
     6661 tctaagcata gttctatcca tatcaaatcc atagaacata ccattattaa aatgctcctt
     6721 cagcccttgc actaagaaaa gacttgaatg attatccctt aagtattgtt gtgatgatac
     6781 taaaaaacct gctgaacctg ctgccgggtc tactataata tcttctggtg ttggtttaat
     6841 aagtcttact atcatatcta ttatgtgtct tggagtccta aattgaccat tagtacctgc
     6901 tgtagccact ttggataata aatactcata caaatctccc tttgtatctc tatccttact
     6961 taattctaaa ttactaatac catcaactat tttagataac ataagaggcg taggaatttt
     7021 aaatattgca tctcccatgt actttgcata agctgagtct ttatttccat gtaatttttt
     7081 tatgaaagga aacacttcat tttgtactat ttcatacatt ttttctgaat ttccttcatt
     7141 agaaaactta ctccatctta aatgttgatg ttcatcatta aatatccttt caaaagaaac
     7201 tcccaaaaga gttgcttctg cttcattttt tgtttctact tcatccaatc ctttaataaa
     7261 taaaagatat gtaaattgtt ctataacttc taatggatta gttaatccac cagtccaaaa
     7321 tgtctcccaa atcttgtcta ctttgctcct taattctcct gtaatcatat atgactttcc
     7381 ctccagtgtt cataaaataa tttaaatata tattttagtc gtacgcctta atagttagtg
     7441 tcttatgtta aatccaagtt tttactacac agttattaaa ttatttttcc tatttacatg
     7501 ttttagttaa aaaactaatg caatactctt attttatgat tattatatat accatacaaa
     7561 attattttat catatatcca tcatagaagt catctacctg gataattctt accatattat
     7621 aactttttaa tattaattta aacttagaaa gaactttttt atttaattat atcatcttcc
     7681 ttatttcaaa tttctcaatt acaatattaa tcaaaaaata aaataagatg ctgtctcaaa
     7741 atagatataa tcttcttttt tattgtcaga atgatgaaaa tatatttaat ttcatacaaa
     7801 aaagagtgcc tcatgaacta aaaagttcat tttgagacac cccttaattt aatcttactt
     7861 attctttccc tttattatta acacttcact gtctctattg cttttcctac acatccacaa
     7921 acttccatcc tactcttaac aaaatcttta actgactcta aacctttttt accatatttc
     7981 ttagggtcaa ttacctctgg gtcagaatta agcacattct taactccatt gctataagca
     8041 atccttaatt cagttgcata attaaccttg caaatccctc tttttataga ttccttaata
     8101 acttcatctg gtattccaga cccaccatgt aatactaatg gaactgatac aacatctcta
     8161 atttcagata atctatctaa atcaagcttt ggctctccct tatataatcc atgtgccgta
     8221 cctattgcta ctgctaaaga tgatacccct gttctctcta caaactcttt tgcttctaat
     8281 gggtctgtat atgctccccc atcaccacca tctaaatcgt cttccttacc tccaacttta
     8341 cctaattcag cttcaacagg tatatctgaa ggcatacatg catctacaac agatttagat
     8401 attgaaatat tatcttcata actttcatga gaaccatcaa tcataataga agtatatcca
     8461 actctaagtg cctgcatagc tagtccaaag cttgaaccat ggtctaagtg cattgccaca
     8521 ggaacacttg ccttctctga tgctaccttt acattcgcta aataataatc tagtcctgca
     8581 tactttattg ttgaaggtgt tgtttgcaat attactggtg atttaagttc ttcagcagcc
     8641 tctattactg ccataaccat ctccatattt tcaacattaa atgcacctac tgcataatgc
     8701 ccttcttgtg catctaataa tatttgttta gttgtcacta atgccataat ttctcctcct
     8761 atatgtttat actgatttaa tttcttcgtt atttaatttt gatactattt ctctacttgt
     8821 tttgctattt aataactcat ttaaaaattc ttcattcatt aattttgaag atagttgaga
     8881 taatgttttt aagtgtaaat tatcaccatt atctgtagct gctatcataa atataatatg
     8941 tgccaagctt ccatcttctg attcaaagtc aaacccttct tttgatacac ctactgctac
     9001 acgtggtacc tttactgcat ctgtttttgc atgtggtatc gctatcccca taccaaaacc
     9061 tgtagatgat atttcttctc ttttaaggat ttcttcttta aacttctttt tatctaaaag
     9121 aacaccagat ttatcaagta ttccaatcat ttcatcaatt acatctgttt tattttttcc
     9181 cttaacatct aaaattactg tttcttcgct atataaagag atattattag atacgttttc
     9241 cgattctgca ctttcatttt tatgttcatc ttttgttgca tttgtatttt ctatagtatt
     9301 tttctcattt actactttag gtgtaaccat catataaagt acagcaccta ctaatgaacc
     9361 tacaaatata caccctaccc ataaaagtgg cttattgact aatcctagaa ccaaaaaccc
     9421 tccatgtgga gctggtactt ctattttcat tatataagtt aaaattgatg atattgatga
     9481 tgcaatcata agtactggca acacttttaa tggatttttt gctgcaaaag gtattgcccc
     9541 ctctgttata tgagtactac ctagtatata attgattaat cctgctgctc tatcttcttc
     9601 tgtaaatcta tttttaaata aagtagatgc tattgctatt accaatggtg gtgttataca
     9661 agctgcagat acacctgcca taaataaata atttccctgt cctagaagca tagttccagt
     9721 tacatatgct gctttattta ctggacctcc catatcaaat gctgacatac atcctattac
     9781 tattcccaaa attataggac ttgaattttg taaccccgat agaaagttca tcatagcatt
     9841 gttaagtgct gaacatggcc caccaagtaa caacattgct acacccacaa gacctacgct
     9901 gacaatagga attataaaaa tcgatttaag accttctaat tgtcgtggta attttcttaa
     9961 caaatgtaca aatagtttaa ctaagtaacc tgctaaaaat cctgatatga ttccacctaa
    10021 aaaccccgaa cctgtatcaa atgatatcat tcctccaaca agtcctgcca acataccagc
    10081 tcttcccgat atagattgag ctataaatgc tgccaataca ggaaccatca agttcataga
    10141 atatccacca acagatttta atgtagctgc tatttgatta tattgttctg aatttgggtc
    10201 aaatgaataa atcccccata aaaatgatat agcagtaagt actccacctg ctactactaa
    10261 aggtaacatg tgagatacac cattcattaa gtctttatac aacttttgac caaacgataa
    10321 atttccttct tctgcaattt tactgcttga taagtttcct tttctaatag ctacttttcc
    10381 atcaatacat ctttgtataa gttcattagc tttatgtata ccttctttga caggtacttc
    10441 tattactggc tttccattga atctgtccat atcaacattt ttatcacaag caactattat
    10501 tgcatctgcc atttcgatat cttttgctgt taacttattt tctacaccta tagctccatt
    10561 cgtttcaact tttatgtcac accctaattc cctggctgaa ttttttaatg cttcttctgc
    10621 cataaaagta tgagctattc cagttggaca acctgttacc cctagaatca ttttctttcc
    10681 catttttata ccacctttct gactcttata ttttttatat aatcttccac attctccaaa
    10741 ttacctatac cattactttc agccacactt gctcctgttg ctgctgacct ttttaatgct
    10801 tcttcaatat gttctggatt ttccaaccat atacttaaaa aagcagctaa tgcagaatca
    10861 ccagcacatg cagagctgac tactgtaact ggttgagcac tagcataata aattgatttt
    10921 ccattataaa aataagaacc tttttctcct aaagttagca atatattttg agcacctttt
    10981 aaatgtagta atttaagtac atccttaata tcttcttcat ctcttacaat aattccaaat
    11041 atatctttga tttcttcatc atttggtttt atcaaatatg gattatactg tagtaactcc
    11101 tttaattttg gtgaacttat atcgagtatt gtctttatac ttttattttt acaaatttta
    11161 aatatatcct catagtaatt atcatcaata ccaggaggta gacttccact tattgataag
    11221 taacttatat cttctaatga ctgtatctta tctagcatat ctagtttttg tttttcattt
    11281 acatatgacc cagagtttac aaatttaaat tcatcacttc catcatttaa aaatatattt
    11341 atacgagttg tatcttctac ccaagtaggc aatacgtcaa atcctctctt ttcgctttct
    11401 tcaactatat attttcctga aaatcctcca aaaaatccta atatcttaga ttctatacca
    11461 taatgaccta gtacaaaagt tacatttaat ccctttccat taggagaata tacagcattt
    11521 gatgtacgat ttactaattt tctctttatc ccattagtag atatattcat atctattgca
    11581 ggattcgtag ttaatgtata tatcattgtc ttcccccttg ctcgtgctgt gtatgttttc
    11641 attataataa aaaagtctat gaaaatcttt tcacaaatag aaaatcgatt ttcatagact
    11701 tttcaattta attttcagaa ttattatatc tgaccatata atctataatt atcctagata
    11761 taatatacaa gggcaatctg gaagatactt catattcaga aattgatata tgttcatgtt
    11821 tgtatcctac aatatataaa tccgaatact ttataagttt tgcactttca ctacaacagc
    11881 aagttataac cttagctcca actaaattgg cattctgtgc tgattctaca agctctatgg
    11941 tatttccatt caatgaaaat ataattataa gttcgtcttt ttttattgat ttagatttta
    12001 ttttcattat gttggggtca tttataaaaa caacattaaa atcaagtaat tgcaatttaa
    12061 gactaaactc ttttgctaca tattcagtaa gccctcttgc caaaatatat atcttactag
    12121 atttatttat agtttttatt atattgatta gtgtagttaa agatatttgt tctataactc
    12181 tctgtgcttc tataaaggat ttatttatta tatcccccat attttgtata tcatcatttt
    12241 catcttttac tgttaatcta tacctaagtt cattgaatcc atttaaacca cattttctaa
    12301 ttgctcttga aacggttgct ggagatgaat atgtttcatg agcaatctct actatagata
    12361 attctggtaa tattgattca ttttcattta taaatttaat tatttcaagt tccgtcttcg
    12421 ttagattttc agaattactt ttgtttagct caattatcat atgttatctc cccttatata
    12481 agttttattt gtctaaattt atcgagtaat ataatatatt acctcttaac tacttatttt
    12541 tatatacaat taagatttag tcaatgtcta tatatgtaaa tttataatat aataattttt
    12601 tgcttaaagt ttattatgtt atcttatagc attttaaaat aaaaaagaat tgtacctcta
    12661 ccaaaatttg gatgaagtac aatagatatt cctcatactg atatgtttat gattaaaagt
    12721 ataaatataa atttcgctat gaatcatata attaataaat cattttattt acaattataa
    12781 cc
//
LOCUS       DAEOSP010000116        12710 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.117, whole genome shotgun sequence.
ACCESSION   DAEOSP010000116 DAEOSP010000000
VERSION     DAEOSP010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12710
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.117"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(180..1430)
                     /locus_tag="KRH54_002423"
     CDS             complement(180..1430)
                     /locus_tag="KRH54_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBG8580078.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1462..2403)
                     /locus_tag="KRH54_002424"
     CDS             complement(1462..2403)
                     /locus_tag="KRH54_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBG8580079.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2643..3719)
                     /locus_tag="KRH54_002425"
     CDS             complement(2643..3719)
                     /locus_tag="KRH54_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBG8580080.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(4092..4607)
                     /locus_tag="KRH54_002426"
     CDS             complement(4092..4607)
                     /locus_tag="KRH54_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBG8580081.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(4970..6040)
                     /gene="leuB"
                     /locus_tag="KRH54_002427"
     CDS             complement(4970..6040)
                     /gene="leuB"
                     /locus_tag="KRH54_002427"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBG8580082.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(6047..6538)
                     /gene="leuD"
                     /locus_tag="KRH54_002428"
     CDS             complement(6047..6538)
                     /gene="leuD"
                     /locus_tag="KRH54_002428"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBG8580083.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(6553..7830)
                     /gene="leuC"
                     /locus_tag="KRH54_002429"
     CDS             complement(6553..7830)
                     /gene="leuC"
                     /locus_tag="KRH54_002429"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBG8580084.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(7787..9529)
                     /gene="leuA"
                     /locus_tag="KRH54_002430"
     CDS             complement(7787..9529)
                     /gene="leuA"
                     /locus_tag="KRH54_002430"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBG8580085.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(9653..9899)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10047..10493)
                     /locus_tag="KRH54_002431"
     CDS             complement(10047..10493)
                     /locus_tag="KRH54_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580086.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(10514..11233)
                     /locus_tag="KRH54_002432"
     CDS             complement(10514..11233)
                     /locus_tag="KRH54_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBG8580087.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLKHLDYIIASL
                     HRPCIASGTKEKNTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(11351..12625)
                     /locus_tag="KRH54_002433"
     CDS             complement(11351..12625)
                     /locus_tag="KRH54_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBG8580088.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAKLQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
BASE COUNT         3828 a         2444 c         1311 g         5127 t
ORIGIN      
        1 ttttcatcct caattatagc atagaatatg actatctata tcctaaaatt atccatttct
       61 aaaaaaaagg taactattat taagaatatc aacttataga atttatattc cacatacgat
      121 tttcaatcaa aaaaacaagg gctaaaaagt gaattgtgca ctttttagcc ctttattaat
      181 tattcattta tttcatgtaa gaatagacca catcttactt taggttcaaa ccatgttgac
      241 ttagctggca ttatcttatt tgcatctgct actcttatta aatcttcaat actagtagga
      301 tacattgcaa atgctacttt catatcttca ttaactcttt tttctaattc ttcaagacct
      361 ctaattccac cgataaaatc aattctttta tctactctag gattatcaat tcctaaaata
      421 gcatcaataa catagtcttg taatatagaa atgtctaatg agtcaacagg gtcttctatt
      481 tctaataaat ttttatttgc tttcatttta taccacttat cacctaggta cattccgaaa
      541 gttccttttt tgtcaggttt atatttttct tttccttcta tttcttctag ttcaaaattt
      601 tcttttattt tatttataaa ttcttcctcg cttaaaccat ttaagtcttt gataacccta
      661 ttataatcca aaaccattaa attctcatct ggtgcaatca ttgctatgaa gtaattaaat
      721 tcttcttttc cagtataatt tggattttta gctctcattt ctttggctac attttctgct
      781 gaagctgtcc tatgatgacc atctgcaatg tatagataat ctaatttttc aaatttatct
      841 actaggttat ctattatatc atcattatca atcttccata ctgtatgctc tatcttgtca
      901 tctgttatga aatcatataa aggttcatta ttatccataa aatcattgat tatatcatct
      961 atcacccttt gatgtttgta agttacaagt atagttcccg tattagcatc acagtatttt
     1021 atgtgtttag ttctatcaat ttccttttct ggtcgagtgt actcatgttt ttttattgtt
     1081 ccattcaagc tttcatcaac tgatatacat gctactatac caacttgagc tctaccatcc
     1141 atgatttgtt tatatatata tatacactct ttgtcatctt ttactagaat ttcttgtttt
     1201 cgaaactcat ctaaattttc tcgcgctttt aaataaactt tttcatcata taaatctata
     1261 ttttcatcta aatcaatttc tgatttatct atatgcaaaa atgaatatgg attcccttct
     1321 gcaattcttc tagcttcttt actattcata acatcgtaag gaagtgctgc tactttatct
     1381 actatatatt tatttggtct aatcgcctta aatggtttaa tcgttgccat tactaatttc
     1441 ctcctaatta tacaaaatat tttataatgc tacccctatt agattgttga agtcaagaaa
     1501 gaaatcttga atcacattta ctatttcgtc tcctattctt tcttgagctt ctactgtaga
     1561 tgccccaata tgaggtgtag gagaaactct tggatgattt actaagtcta aatttaatgt
     1621 aggttcttgt tcataaacat ctatagcagc tgctgataat ttaccttcat ctaatgcttt
     1681 tagtaaatct ttttcatcaa ctaaaccgcc tcttgcacag tttattaaat aagccccttt
     1741 cttcatctta tttatttctt tttcagttat tactgccccc ttattcttat caaatggtat
     1801 gtgtaaagat aagaaatctg cattttctaa aacttcttcc atatcacaga atttataatt
     1861 gttaaagcct tgcgcctcac ccattatgtc agtatatata acattcatac ctaataattc
     1921 tgctctcttt gcaacttctt ttgcaattct tccaaaacct ataagtccaa gtgtctttcc
     1981 attaatttct gtacctttat actttttctt ctcccatttt ccatctctca tagttacatt
     2041 tgcagtattt atgaatcttg caagaacaaa taattgacct attgtaagct ctgcaacaga
     2101 tatactgctt gcatttggag tattcataac ttttatgcca ttagcttgag catatttaac
     2161 atctatatta tccagaccta ctccacctct tactatcaac ttcaactttt taccctctaa
     2221 tgctttatct attacatctt tagtaacttt agtagctgaa cgtactacaa gtacatcaac
     2281 atctttaagt ttatctccta aaacatcttt ttcataaaac tgctctatca cattaaaatc
     2341 taactctctc aattttctcg ctgcttcttt ctctattcca tcagtaacta atatattata
     2401 cataacaaac tcctccaatt tttattcact taatttttcc aatttttatt ttttaaagta
     2461 ttttcatcaa taaaaaactt tacttaattc aactttctgt ttatataatt ttcaaaatat
     2521 ttttttatta aagtcatgtc aaatttttct attgtttgtt aaaaatttaa tatgtaattt
     2581 ttataaaata ttatttatta tatattctaa atttatctta aaacatacat atttttacaa
     2641 tattatttat taaaacctaa tatatcatca atattatcta ataattcttg tacatcttcg
     2701 acagtatagt cacccatatg tgaaattcta aaagtttttt cttttaaatc tccatagcca
     2761 tttgctattt ggaatcctcg ttcttgtaat tttgaattta aatttgatac actaatacct
     2821 tgagtatttt ctataacagt aagtgtattg gataagtgat tttcattggt aaaaatttgg
     2881 aagtgttttt tagcccagtt tctaactaag tttgccatgt cttcatgtct gttgaatctg
     2941 ttatctaggc cttcatctaa tatattatct aattggaaat ctaaagcgaa catgtgagaa
     3001 agagatggtg ttgaagggta ttggtagttt ttcttgatta agtattcata cattgctaac
     3061 aaatcaagat atactcctct aaaaggtact tgcttagctc tatcgattgc tttttgagaa
     3121 aatgtgcaaa tagacattcc tggtggtaat cccagtgctt tttgtactga tgttatacaa
     3181 atatctattc caatcttatc aacctcgact tttattccac cggctgaact tacagtatct
     3241 acacagtaga ttacatcttc atacttttta acaacttctc caatttcttc tattggattt
     3301 cttattccag ttgaagtttc attgtgagtt acagttatta aatcgtattc tccggtagaa
     3361 agcaccttat caaccatttc tggcgttgtt gcctctccta attcaacttt aaatatatcc
     3421 gctgggacat tattagccac tgccattttg taccatctat caccaaagga tccacaggaa
     3481 aatactgctg cctttttgga tgtacaggaa cgaatggagc cttccattaa accagttccg
     3541 gatgacgtgg ataaaagtat cgtgttgttt gtatagaata gtttttgcat cttttcactt
     3601 atactctttt ggagcataga tgcatctttt cctctatgtc ctatcattgg tgtagccatc
     3661 ttttgcaaga cttcttcttt aacatcaata ggaccaggaa taaataattt cttactcatt
     3721 ataattcccc cctttttagt ttgtaatatg gatagcgttt ttactgtcct tatttttaat
     3781 aactataata tcaagctgaa ttaactatgt caacataaaa tttatttttt atatataatt
     3841 tattttatat attttatgaa aacattcatt ttctaccttt tcataagtct gaattttctg
     3901 aattattctc tcaaaaaaaa tatacttttt atctatataa attaattttt ttagtatgta
     3961 aatttgacgc tattacgata aatcttttta gtataactaa tttattttta tcttgtattt
     4021 ttattgtatt tttgaatttt attttttcta aatataaaat ttatgtttta aaatgtaaaa
     4081 tttttattta atcaaagttc aatatattcc actttttctt tcgattcttc aaaaaaccat
     4141 tcttcattag aaaaatcacc ttctgcaatt tgtccaatag gcattttcat atactttcca
     4201 gttgctgtca ctgctatatc tccatttggt agaattattt ctcctgtacc ttcaaataat
     4261 tttctgtttt ctttagttat tcttccaaca acttttatag tagcatctgt tggtactggt
     4321 ttcttatatt tcaactctaa agatactgtt actccccata tttggtcatc atttatactt
     4381 acagctcttc caatagtctc atccaatatt gcagcagaca tacctccatg aactctccca
     4441 ggataacttt gatgccaatc ctttgtatta caaattgata caagctctcc attttcaagt
     4501 tcataaaaat ctgctttaag tcctagctca ttttgtgttc cacaaactaa acaagattta
     4561 ctactatttt gcttcttcaa aactttatac tttgccaaaa aatccatctc ctaaccgaca
     4621 ttagtagtcg cttaatatta aataattcta aaatatatta tataatatta ataaaatccc
     4681 tgcaaacttc taataataaa aataggctac ttcaatatga taagtttaat ttaaagtatt
     4741 attttccaaa aaataacttt atcaaaatag aaactcaaag tagatataag cctctttttc
     4801 gttattaaaa tagtaaaaat atattttact taatataaaa aagagtgtct cattaactaa
     4861 taaagtttat tttgagacac ccctcataaa acaaattata ctacaataga tttttactaa
     4921 ttctacaagt tatatattca aattatatta ttcaaattaa actataattc taaatttcat
     4981 ttactattaa ttcgcccatt tcccttgttc ctacaacatt tcccacacca ttatagatat
     5041 caatagttct ataaccttta ttgagtacac tttgtacagc atcttcaata cacttagctt
     5101 cttcttctaa gttaaaacta tgtcttaaca tcatgcttac agatagaata gtcgcaattg
     5161 gatttacaat atcttttcca gctatatcag gtgcacttcc atgtattggt tcatacatac
     5221 caaaactatc ttctctaagt gatgctgatg gtaacatacc tatagaaccc gtaatcatag
     5281 atgcttcatc agatattata tctccaaata tattattagt cactattaca tcaaattggc
     5341 tagggtcttt tacaacttgc atagcagcat tatcaacata catatgtgac agttctacat
     5401 cttcaaactc tttagctaag tcttccatta tacttctcca aagtcttgaa ctctcaagca
     5461 catttgcctt atctacactt ataagttttt tatttctatt tctggctatt tcaaaagctc
     5521 tcttaccaat tcttcttatt tcattttcgt tatatatttc tacatcataa gcattttcga
     5581 taccatcaat gatttttctt ccacgttctc caaaataaat gccccctgtt aattccctta
     5641 caacaacaaa atcaatgcct ttctcaacta tatccgttct aagtggtgaa gcttctttta
     5701 tactttcata cattgtagct ggtcttaaat ttgcaaataa acccaatgac tctcttaatt
     5761 ttaaaagacc agactctggt cttttatctc ccttacattt atcccacttt ggtcctccaa
     5821 cagcaccaag caatacagct tcgttttttc gacaagtttc aacagtttta ttaggtagag
     5881 gttctccagt ttcatcaatc gcgcatcctc cagctaatac atattcacaa tcgaatctat
     5941 gattaaattt acaacaaatt ttatttagaa cctttatacc ttcatctata atttccggtc
     6001 caacaccatc accttttatg actgctatat tacaattcat acttcactac cccctatttg
     6061 cttttattga gttaattaag ccttcattta atataatatt ttgcataaac tcaggaaatg
     6121 gttctgcttt atattctttt ccttttgtta tattttttat cacacctgta gaaaaatcta
     6181 cttctatatt atccccttca tctatgttat tggcagcttc ttcacattca agtataggaa
     6241 gccctatatt tatagagttt ctaaaaaata ttctagcaaa agtagaagca atcacacagg
     6301 atactccact ttcttttatt acaattggtg catgttctct tgatgaacca caaccaaagt
     6361 ttttagttgc aactataata tctccttttt ttaccttaga aataaattta tcatcaatat
     6421 cctccataca atgcgttgct agttctttat aatcagctat atttaaatat ctagctggta
     6481 ttattacatc tgtatctata ttgtcaccga atttaaaaac acttccattg gcaatcataa
     6541 attttacctc ccttaaatta tttctgatgg ttttgatatt tttccagtta tagctgatgc
     6601 tgctgctaca gctgggcttg caagatacac ctctgaatca acatgcccca ttcttcctac
     6661 gaaattccta tttgttgttg aaatggattt ttctcctgct gcaagtatac ccatatgtcc
     6721 ccctagacat ggaccacaag ttggtgtaga aactgctgct cctgcctcta taaaagtggt
     6781 aatataccct ttttctatag cttcaagata tactttttga gttccaggaa atatcataac
     6841 tctaactcca tctgctactt ttttgccttt cattatctca gctactactt caaggtctga
     6901 tattcttcca tttgtacaag agcctattac aacttggtct atctttatat ccttatctat
     6961 ctcatctact gttctagtat tttcaggaag atgaggaaag gctactgttt cttttagttc
     7021 agctaaatct atttcgtata cttcatcata gactgcatct tcatctgctt catatatatt
     7081 tacatctttt gtcatagttg aacacttatg tgaatttata tattccatag tcttatcgtc
     7141 tactggaaaa attccatttt ttgctcctgc ttctattgcc atattacaaa tagtaaatct
     7201 atcatccata gataagtatt ctactccatc tccacaaaat tccattgact tgtaaagtgc
     7261 tccatcaaca cctatctccc ctattatatg aagtattata tctttaccac ttatccattt
     7321 atttggttta ttcttcaaaa caaattttat tgctccagga accttaaacc aacattttcc
     7381 tgtagccata cctgctccca tatcagttga acctacacca gtagaaaatg ctcctaatgc
     7441 tccatatgtg caagtatgag agtcagcacc tataattaca tctccagcag ttactatacc
     7501 tttttcagga agaagaacat gctctattcc catatctcct acatcaaaaa aatttacaac
     7561 tccttttgat tttgcgaaac ttcttgtaaa tttacattgt tctgcactct ttatatcttt
     7621 attaggtgta aagtggtcta aaaccatcac tactttagtc ttatcataaa catcttcaat
     7681 tcctaatttt tcaaattctc taatggctac tggtgatgtt atatcattac ctaaaactac
     7741 atctaaatct gcttcgatta attgcccttt cttaacttca tttaatttag catgtttggc
     7801 taatattttc tgcgtcatag tcatccccat tttttatttc ctcctcaata taacttttat
     7861 ttattgcatt cataagtgca ttaatagctg ctgtatttat gttttccgat atacttacac
     7921 caaaatagac ttcattattg tgctctattc ctacataagc tacccctctt gaatgtgacc
     7981 cttcctctaa tgcatgttcc cagtaatatc taaatttata atctttcatg ccacattgct
     8041 ttagtgcatt attaaatgaa tctacaggtc catttcctat accaacaatc ctttgttcat
     8101 ttcccatata acttattctt gcagtcatct caatcatgtt agtatcatta gaatcatcat
     8161 tttcatagtt taattcatcc attgatttta ttttatactt cttaacttta taagggctct
     8221 caacttcaat atattctttc ttaaacaaat taaatattgc ttcactacta agttcttccc
     8281 caattctatc agattccatt ttgactacat ttccaaaatc agaatgcata ttcttaggta
     8341 tcataaatcc ataatctgtt tccattatat aaacagctcc tccttttcca gattggctat
     8401 ttatccttat aatttctttg tattctcttc caatatcatg agggtctatt ggtaaatatg
     8461 gaacttccca gtaatcatta tctctgtttt tcattgctat catgcctttt ctaatagcat
     8521 cttgatgtga acctgaaaaa gcacaatgta caagattacc tgcatatgga tgtctatcat
     8581 gtaccataag ttttgtacag tcttcatata tacctattat cttatctata ttgctaaaat
     8641 ctagctctgg gtctattcct tgtgtataaa gattaagtcc cacatttact atatccatat
     8701 tcccagttct ttcaccattt ccaaatagtg ttccttcaag tctatcggca ccagccaata
     8761 atccaagttc acttgcagcc gtacaagttc ctctatcatt gtgagtatgc aaacttagta
     8821 taatagaatc cctacaactt atatttttac aaaaccattc tatttggtct gcatatacgt
     8881 ttggtgttgc catttcaact gttgatggaa gatttattat tacttttttt tgaggagttg
     8941 gtttccaaac atctatcact gcctcacata tttcaagtgc ataatccatc tctgttcctg
     9001 taaaactctc tggtgaatat tcaaaagtaa attctgtttc tggatatttt tttacttctt
     9061 cattaaataa ttttgctcct tcaactgcta tatctattat ttcttgttta ctcatattaa
     9121 aaaccacatc tctttgaaga acagatgtag agttatataa atgtataatt gcttttttac
     9181 atccccttat tgattcaaat gttttttcta taagatgagc cctagattgt gtaagtactt
     9241 gtatagttac atcatctgga atcatattct catctattaa ttttcttaaa aatgtatact
     9301 ctgtatttga tgctgatgga aatcctacct ctatttcttt aaatcctgta tcaataagca
     9361 ttttgaacat tctgactttt tcatttacac tcataggtgt tggcagtgac tgatttccgt
     9421 ctcttaaatc gacactacac caaattggag ccttaaatat ttcattatct ggccatttac
     9481 gatttactat ttgcatttta tcatattttt tatactttcc acacttcatg ttatttcccc
     9541 ctttttttgt tttttgtatt cagtatacct catatcgacg caatcctgca caattcataa
     9601 ggataaatgt caattttatc attagaataa aaaaaacctc atctctaata aaattagaga
     9661 cgaggttttg ctcgcggtac cactctattt agctttctaa aaagctccct tagtaatagc
     9721 caatacaata attataatga ctatatttcc tataacgtgg aattactccg attattctaa
     9781 gatttaaatc ttagaaaaca gctcctagac aagttcagaa ctatcaacta ctaaatcaca
     9841 ccatccttta gctctctgaa agtttcattg ttcttactac ttctattcat cactttttta
     9901 atcatttaaa ttttctaaat attttgattt aatagttatt atatacacat taaaatgtat
     9961 tgtcaacaca aaaaataatt atctcaattt tttatttttg ataagcaatt ctataaaaca
    10021 tgctaactat tgaaaacagt atgatattat gccttaaact ttttcatctt ttcatctatg
    10081 atattcaata attttttttc atcttccaaa ctcaatgccc ttaatctaat tttataatta
    10141 gattcgtcag tgtatatatt taatatattt tttactactt ctattttttc tattatctta
    10201 taattaaact tttgactttg tttagatttt atactgaata tacattgatt atttattaat
    10261 attgaatgat tatcgagagt aatatatact tttccctcat atttatttga aaaatcacga
    10321 ccttgtttaa atagaaaaac tgctgttatt atacataaaa tatataatat tgtcgttgaa
    10381 tatataaaaa ttgtagctaa aattataagt gctaaaacca tatatatagt cattcctttt
    10441 ttaaaataac cattgtcata ataattcata tagataaact tatttttact caaatttttt
    10501 cactcccaag attctaaaaa tttatatcaa aaaattctat aatttcatct tcatgataat
    10561 ttatgactaa ttcatctgga aaatctactg cttctaaaac tttttctgca ttttcaaatt
    10621 ctcctataga ataacatata tgagagtcag taccaagaat tactcttgca ccatatgttt
    10681 tacacaatgt caacatatga cttacatttt cccaagtacc cgttctatga gagtttgaac
    10741 ttaatgatga attatttatt tctaaaagga tatttttatc tttagctttt tttactatag
    10801 gttcataatc taaaggatac ctactgtcat ctggatgtcc aattattttt accttaggtt
    10861 tatccataac ttttaaaata gcatttgtat ttttttcttt tgtaccagag gctatacaag
    10921 gtctatgcaa acttgcgatt atataatcca aatgtttcaa catatcttct tgaacatcta
    10981 aattaccatt ataatccatt atatttcctt ctattcctct aagaatcttt acgccttgca
    11041 cttcacgtgg aagcaaatgc aaattataaa aatagaatgg atgtggtcct cctggcatat
    11101 taggtccatg gtctgatatt cctaagtatt ttatattctt cttactcgcc tcttcgacat
    11161 tttccttgaa agtactatgt gcatgtccac ttattagagt gtgacaatgt acatccataa
    11221 tagacttttt cattaaattt cctcctaata ttttacataa attcgtgaaa atagctaaag
    11281 ggctatttca aaactaaagc tgtcataata gacagtgtaa cgttttaaaa cagccccatt
    11341 ttcaattatt ttacacttct aaatctttaa atttatcttt aaataattct ccaatacttc
    11401 ctaaagattc ctctacttct aggtattctg ttatatcctc ttctggctct ctagttgctt
    11461 cttttattga aaccaacatt cttttagtct ttggtttaaa atcaagaatc ttaactttaa
    11521 cctcatctcc aatattaaca acattagtaa ctttagccac tctagtatca ctcaattcag
    11581 atattggtaa atacgcatct acaccttctt taatttgaac aaaagcccct ttttctatta
    11641 ttctaagaac tttagcattg acaacatcat caatttgtac ttcttttgct attaaatccc
    11701 aagggtcatt atttatgtct tttaatgcca aggcaatttt tttagtctct ttatttacat
    11761 ccaacacata aacttctact tcttgtccct ctgaaacaac atcttcaaca ctttcaactc
    11821 ttgtccaagc taaattattt atatgtgcta atccttcaat cccaccaata tccacaaatg
    11881 caccataagg cattatcttt gtcaccttac catctctttt ttgtccaact tcaagagaat
    11941 taaataagtc ttctttagct gctttgatac gagctttttc ttcttctctt tcttgcttaa
    12001 ttcttgctct ttcagcttct ttttctgcct taatttgagc tcttctttct tctctctctt
    12061 tatttatacg ctcttgtaat acatctctat gagatgctac aagtctattt tttctaacat
    12121 ccaattcttt tatataaact tctaagttaa gaccaacaaa tttagatgta tctgtaataa
    12181 attttgtatc aagttgagaa attggtataa aaaatgtata tgtattgtaa ttagcaaata
    12241 cacctttatc tatattcttt tcaacattta cagttattat tctatgttca tcataagcct
    12301 tttgtaactt ggcaaaatca tttcttttgt caagttgtaa tttagataaa gttattgtac
    12361 catctttttg agatactttt gttattacag cagttatttc atcacctatg tggtatatat
    12421 cttctatata ttgatttttt tctatgttca attcacttat aggtattatt ccatcaaacc
    12481 catagtctaa tcctaattgt acttcatcat ttctcactgc agttattttt ccagttgtca
    12541 attctccaac tttcactttg ctgaatactt gttcttgctg gtcaagtaat tcttgcattg
    12601 ttaaatcatt ttccattata aacatccttt cctcttattg attatcatat ctgtaaatat
    12661 tataccatta atattgcaaa tacttacatt tttcttacta taatttttaa
//
LOCUS       DAEOSP010000117        12637 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.118, whole genome shotgun sequence.
ACCESSION   DAEOSP010000117 DAEOSP010000000
VERSION     DAEOSP010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12637
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.118"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(549..1301)
                     /locus_tag="KRH54_002434"
     CDS             complement(549..1301)
                     /locus_tag="KRH54_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBG8580089.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNDLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(1360..3015)
                     /locus_tag="KRH54_002435"
     CDS             complement(1360..3015)
                     /locus_tag="KRH54_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8580090.1"
                     /translation="MVDFSESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKQCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(4288..5367)
                     /locus_tag="KRH54_002436"
     CDS             complement(4288..5367)
                     /locus_tag="KRH54_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8580091.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIVNQNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(5451..6107)
                     /locus_tag="KRH54_002437"
     CDS             complement(5451..6107)
                     /locus_tag="KRH54_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8580092.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            6429..6635
                     /locus_tag="KRH54_002438"
     CDS             6429..6635
                     /locus_tag="KRH54_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580093.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCLAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(6788..7216)
                     /locus_tag="KRH54_002439"
     CDS             complement(6788..7216)
                     /locus_tag="KRH54_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG8580094.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(7692..8630)
                     /locus_tag="KRH54_002440"
     CDS             complement(7692..8630)
                     /locus_tag="KRH54_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBG8580095.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNGKR"
     gene            complement(9144..9956)
                     /locus_tag="KRH54_002441"
     CDS             complement(9144..9956)
                     /locus_tag="KRH54_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8580096.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFTKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWINMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLVEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
     gene            complement(9979..10611)
                     /locus_tag="KRH54_002442"
     CDS             complement(9979..10611)
                     /locus_tag="KRH54_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8580097.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFRQL"
     gene            complement(10955..12610)
                     /gene="ilvD"
                     /locus_tag="KRH54_002443"
     CDS             complement(10955..12610)
                     /gene="ilvD"
                     /locus_tag="KRH54_002443"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBG8580098.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEALAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKYVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
BASE COUNT         4030 a         2336 c         1395 g         4876 t
ORIGIN      
        1 acaaaagaat aaaaaaaaat taatttagca taaccttgtt aaatatagat ttgaatatga
       61 atttaaagtt atgtaaaatt aaaaatacag gaatgaaaat tccattcctg tataaaatct
      121 aacttttgag tctcagtaca atcttatttt gagacaatct tttttgtttt atattttctt
      181 atatttaaaa tataacgcat atcttatgca acatattcat atttttcttt atattctttc
      241 atcgcatcat tccaagcgtc ttgtactgtt acacctcttt tattagcaat tttttgtgcc
      301 agttcaatta tcttcatagc tcttcttgat atgttcataa acattcctcc tacaaattac
      361 catatatttc atatatataa atcaaggtta tatttttatg atatataatc cttctattgt
      421 aagattacag tttatatgtg tattatatat gaactatatt agaagataat tagaattttt
      481 ataaagctat atgattagat taaatataaa agctttacaa ttacttataa atttaaaaag
      541 tctataagct aaactaatgt ttgatatacc aattttacga ccataaataa tgccattact
      601 aaaaatattg gttttatcat ctttgaacct ttctttatag caactttagc acctacataa
      661 gctcccatta tcatcactaa tgcaaacact atagcaattt tataatctac ttgaccattt
      721 atcatgaata ataacaatga agttatatta cttgttaagt ttaatatttt tgtatttgca
      781 gaagcatgta aaaaatcata tccatatatt tttataaatc caaaggtcaa aaaagttcct
      841 gtacctggac caaaaaaacc atcataaaaa cccattataa gagccataag catccctagt
      901 cttaaatttt tcttattaac agcttcaaaa ttatcttctt gacctaaatt tttagaaata
      961 aaggtgtaaa tagccactat taaaatcata accacaacca aaacatttaa tatctcttca
     1021 cttattgata atacacatct cacacctaga acactaccaa taatagtaaa tggtactaat
     1081 ttttttaaaa ggtcgttatt aatttttcca gactttgcat atctataggc actacttata
     1141 catcctgctg atgctgcaaa tttatttgta ccaatggcaa gatgtactgg cactccaatt
     1201 gccataagaa caggcatgct tataagtcct cctcctcctg ctatagaatc tacaaaagca
     1261 gctaaaaacc cccctataca taaaaaaata actgaaaaca taaaattcct ccatcattct
     1321 ctttcaattt ttcttacaat atatattata taatcttatt taatacttat tatccagcat
     1381 attctttcta tattcatttg gagttatact attaaacttc ttaaatattt gtgaaaaata
     1441 actactgtta ttaaaaccta ctttcttaca aatatcacta atactcatgc ttctatctaa
     1501 cagatatttt ttagattctt ctatcctaag aagatttata tactcagtta ttgaaacttt
     1561 atttctttca ttaaaaatag aactcaaata atttggactt ataaaaaaaa catctgatat
     1621 actattcaaa tttatatttt ctttataata tttttttata tattcgttta tattttcaat
     1681 tagatttcct ttatcttttc tccaaaatgc tctattctta tcaatacact ctttggaaaa
     1741 ttcaaataat acattccata aatcttcatc actgtttaca agtgatattt ttttattata
     1801 tttatttctt aaataagata taagttttaa atcctctaaa tctctaagca ctgttctaga
     1861 caatacaaat atcatttcct gaatagtatc tctaacatca ttatatctta atttgtttgt
     1921 atctattaga ttataaacac cttctaaatt catattttta atttcttttt caatataaag
     1981 ttctaattca aagggatatt cttttgaaga attattgaac atatctatcg gattaataat
     2041 atactcttct tctaataatc tactatttgc ttttttaagt gcctcttcaa gtcttatatt
     2101 ttcttttcta ctcttcaata tctcctcatc ttttatacta agttgatttt caaaaaaatt
     2161 cctcttagct atttcctgaa taaattcaga catcattaat aaattttctt ttaatacata
     2221 taacctactt ttaggattta atggaaattc attgtaaatt ttgtttatgt attttatatc
     2281 aataccagaa ctacttgata attcagataa atttttatct atatcaatag acttatatac
     2341 tataaaatta ccacattcta tatatccaac tatattttct tctaatacta taggtatctc
     2401 taaactggaa acaccatact cacaatggta cactttttca aagttatcat tatttatgct
     2461 tttaacaagt tttttagtac actgttttat atttttacaa atatcgcaaa atttattttc
     2521 tttcttagtc tcaaatattt ctttatactt taagtcatat acagatattc taagcttcaa
     2581 tatattagat atttgatttg caagattttc aattatgttt acattaacta actcctgtat
     2641 attgttttca gaaaatacta taggtatttg tctcaaactc gaaaatttat gaggaacata
     2701 agtaattaat attactaaat caattttacc agtgtttata atttcatgtt ctgaatatgg
     2761 tgggcaataa actatagatg tttcctttat attgaccttt ttaccatcaa ttatttgtat
     2821 tccttctccc tgaatcacat ataaaagctg ttcttcactt aaatgaaaat gcttttcttg
     2881 gcagtttccc ggaaaaaatc ttacaattcc agcactaagt ctattgaatt gtgtttttga
     2941 tggttcgtgt atccacataa cctctcccca atcaaatttt tgtacttcat tatttgactc
     3001 actaaaatcc accataaact cacctcgtag tttttgtcta aatatttttt cttcccttct
     3061 tatattaaaa taatatcatc atcattaact ttcattacta taaaatcatc atactctttt
     3121 aatactcctg taattttagt tgcacttagt ttactacaat tcagtttttt aatatattga
     3181 taaattctat aattactttt ttattttgta aaatcagtgt tttcccaaaa taattatatc
     3241 tataaatgaa agtattttta ctgcttacat caatatatgt tatcataatt ttccttttta
     3301 ttgcatatat aaataactgt atataacatt gtccaatact atatagaaat acatctaaga
     3361 aagaatatta ttaaattgtt ccaattagta ttatttttct tataaaaata aaaaaactgt
     3421 tggattcttt tagaaaaatt attttttaca tacctttttt gcttagatag tctatcttct
     3481 cagctatttt gtatatacac tcttctagtt catgatttta ttttaatatc ttgcttatat
     3541 tgctgaaata attgaattct tttatctatc atattgtctc ttatatcata aacatattgt
     3601 ttcttatatc acaaaaatga aagcattctt atatcttcaa tatataaacc tgcttttttt
     3661 aatcttgtaa taaatatagg atactctata ttctcttcct agaaataact aataccactt
     3721 atttttctat tatatagaaa gtaataaatc tttttcttaa taaaattcaa tagtaagtgg
     3781 aataaaattt tatatgaata agaacattat aaaaatactt tatacaatag tcaataaaag
     3841 atattagctt caaattatgt taaagttgta ttatttaaag tgaaaaaaca aataaaaaag
     3901 aaataatagt ataagtagaa aaatatactt aaaacaaggc gaagcctctc ttgtatctaa
     3961 atcaagtaga gaattaaatc taaaaaagaa aataaatata aaagatgact cctgtacatt
     4021 acaagaatca tcttatggtt tcataatcac tccatggttt ttaactgtct gatataaagg
     4081 tctcagttca ctcatagcat gaccaccctt tccgattgct gaccccgtca cttatggaca
     4141 aaatgtccat aagtgatagt cctctcttaa aaccctctaa atagatatat ctcaagcacc
     4201 tcattgaaac cagttgctgg aaacaaactg aagcgttctg gaatagtaaa agaaattgcc
     4261 accatatcca cgttactgtc ctagttgtta ctttgctaac ttcggagcaa tttcctctaa
     4321 caatacgcag tcaaattctg cctttttgct aagaaatggc agatggtctg catctggtat
     4381 aaaaaaaatc tgtttgtctg gcgccactat ttcatcaaaa aatttcttcg ctggaagata
     4441 ggggctatta tagtcatttt ctcccatgat gtagtataca gggacctgat agtcaactcc
     4501 ataatttctt atgtcagctt cttccactaa aaaccgcaat acatcacctt gattgtcaat
     4561 cgcattttga ttgacaatgg ccgtattttt taactctctc aatgaataat acggggttgc
     4621 cagtatactg attatattgc ccaaatccat atttatagaa agcccatact tgctttggta
     4681 tgttcttact gtcataagag ctgccaaata gcttggacca tattttgctg gcggatatgg
     4741 tgcaatagct tccagcgcct tgatatcttt ttgcttccct gcagctttca ccatttcaag
     4801 tgtcttttca tagcccacgc gctcattgtc caccatatta acggtctgcg caatcccaat
     4861 gtacgcgctg taatcctccg gataggtttg cacagccata gttcccagca ctgttcccca
     4921 gctatgacct ataaggataa ttttctcctt gttgaactct tgcttcaaat atgctgtcac
     4981 ctcatggacg tcttgcagaa cacgctccgc gctcatggtc tgcaaaactg ctgccggatc
     5041 gttcgcaaag tatgttttgc ctacgtttcg ctgatcccag ttcaccacag taaaatcatt
     5101 ttcccactca tactggtaca gatgtaaaaa tggcatcatt gaattaccag gacccccatg
     5161 aacccaaagg atgatggggt tgtctgcgtt ttgtccccgg atatataacg cctgctgtat
     5221 gccgccgatc tccaccattt ccatgcggtc gattccagga gcttctattt tatggcttgt
     5281 acgtataatc gtgggggcta tcagacgcaa accggcaaac gccagaacaa aaacagcaac
     5341 tagtatgcaa agtgtgatta atatcatttt aagtaccttc tttcatgaat ttttttagcc
     5401 atagatatct cctctaacat taaaaaaaat atgttgctat tttagttttg ttactctacc
     5461 tgtcccattg gtttcctttc ataaaaaata ttaattgatt ctataatctc atcttccagg
     5521 atatcaactc ctatttgtct cataagaaac tgtaagaatt gaaaccgttt acgaagtgta
     5581 ggaaagtccc cctgaaatac atctgtatca caccaaaagt taaagagcag caaaattacc
     5641 tctgcgcatt catctgggaa ctctgtgatg atgcttctat cctctatgcc ttctcgtaaa
     5701 acatttgcta atattggtgc tagttttttc aaattatcct gtgtaaatgc cagtatgata
     5761 tgcggacttt ctatagcgct tgtaatcatg ttactagatt cttttatcac ctcatccgtt
     5821 aaatgccgcc taactaaact tctaagcttc tcttttgctg taagtccatg catttcaccg
     5881 agccattgat ttactgtctc tattatctgt tcaaagcgcc tttccatgac cacattaaaa
     5941 atatcttctt ttgaactgaa gtgatgaaaa attccgccct ttgacattcc cgaagcatca
     6001 acaatatttt gcatacttgt tttgtcatat cctttttcag caaacaattt tgcagaaatg
     6061 gtaatgatat tttcaatagc ttgctttgga ttgttctttc tagtcattat ataactcctt
     6121 cttattaaaa accgactgtt ggtttttatt atataagcta ttcctacttc tgtcaagcaa
     6181 cattatgttt atggcaaaac aataaggttt ggtggttaga caggtattaa ttagactaat
     6241 gcgactttcc cctagatgga acccttggta aatttgcaat ctttaccggc tttgattata
     6301 tctgtaagca aggcaggtat ggccacacct gccctgtttg accctcggat attcattcct
     6361 atcccttctc tccttagcca ttacaccaaa acgccgctca ttgtctccaa tggcggcgtt
     6421 ttaaagatat gatttctcat gctgctgaaa tatgcaaaaa atataagatg ccctttatta
     6481 tttttcctac aatagaaaag aaaagtattg aagaatgggt taatattgga gcaaatatgc
     6541 tttgtttggc acaagatact acctttatca cttatgttat caatcaatta ttatcttttt
     6601 ataatgagat tgtaccaatt caaaaacaac attaaattat aaatctaatt ttatattaaa
     6661 agagactaat attttatcta tctctgttaa ggatattttc ttatgtatca taatattagt
     6721 ctctcttatt attatatact gctatcatca ttagttgcta tttatactat ttttataata
     6781 tacaaattta ttcagctaac gttttgccta attctataca ctcatctttt gcttcttcat
     6841 ttgggtcttc cattgctgta attccttctc caccaataat tgtagcacca gcattttgca
     6901 tacgttcttc ccagtctctc atccattcac aatttcccca tccatatgaa ccaaataatc
     6961 caatctgttt acctgatacc atagattcta attctgaaac aaatggctcc atatacccct
     7021 cttctagttg ctctgctccc atagatgggc aacctaatgc aaatttatct tcttctttta
     7081 aatcacttgg tttcagtaag ctcacatcta aaacttctgg tgtcttacct tttaacttca
     7141 caccttcagc cacaaaattt gccatttttt cagtatttcc tgttccactc caataaacaa
     7201 tatatatttt actcattttt acctcctgta gtatttttta attaatcaat agttagttat
     7261 agctaactaa catctaaaaa acaaaagatt tctttatctg aaatctcttt atttttctaa
     7321 atatagaata tcatagttga ttttgaaatt caatgcttat tgataatatt tatcatttat
     7381 attttatatt cattttataa tatcaaaaca ctgcatcgta tttctaatct ttcatagaaa
     7441 aatttctaac ttatagtact tttgcatctc ttataaatct tttatatgta taagtacata
     7501 gaaaaacaca ccttttccag atgtgctatc tataatatcg tatatttatt tttcaatttt
     7561 aactagctaa tatgtttata aattttatca cttaatacct ttatacattg atgtaaaatc
     7621 ttacacctaa gtaattttta ttatttgtct cgactctata taatatagct attttatcat
     7681 caaatcaaaa attatctttt gccattattc ttgtatttat agagatttaa atataagtat
     7741 ccatttcctt gttccgttct aggtattcca agcgctctcg tacattttat taattgtgca
     7801 tacagttcag cttcatctaa atctctttca aactcctctc ttgcccattc tttaattagt
     7861 gctaatgagc ctgatacgta tggtgcactc atactggtac cactcataat agcaagttta
     7921 ttatccatat aagtcgatat aatatttctt cctggagcta ccaaatctat tgtagtattt
     7981 gaattactaa acttttcaac caaatagttt tcatttattg cacctacttc tattacctca
     8041 gcataactgg ctggataact atactcactt gtactagaat caccatctcc attattacct
     8101 gctgcacaca ctacagaaat attatttttt actgcttgca tgacagcatt ttttaaattc
     8161 ttatcatctt tgtttccacc aagagacata gatataatat caaccttgtt atttacagca
     8221 aagttaatag cattaattat actttgatat gttccagtac catctttatt taatgctttt
     8281 gctattaata atttacaatc tggagctact cccataactt cattattata gttagatgca
     8341 gctataatac ccgccacatg agttccatga ccattaaaat cctcatatat atttttattt
     8401 ccattactgt catcactaaa atttgcacca ccaattattt ttccttttaa aagaggatga
     8461 gatatatcac aacctgtatc tattatacca actacaatat ttttaccagt ataaccttca
     8521 tcccacattc ccctagcatt catttgtttt attccatatg ggaatttatt ttttgcacct
     8581 cttagatttt catttatttc atacggaatt agtttcattc ttttattcat catatatcct
     8641 cctaaaaatt ttattgctta taaaaatcat gaattttatt ttataatagg tttataaatc
     8701 aaattaaaac ctatatctac ttatttatat ttatgatata ttttttattt ttaggagtca
     8761 tattttattg tatataaaat aatatttaga taactatatt aaaaaataca tgtttgctta
     8821 tatacaaaac ttactaaaat gtctttaata tttttgtata caaacattat attttttata
     8881 taaaaagctc ataaaaattt tgattatatt ttcataagtt aacaaatgtg ggtataatat
     8941 gaatttacaa aattcctctg tttgtataag tgctttacca atacaacaat atataatcac
     9001 attgaagcta tatttgtatt tatagtagat aatgtatcaa aaattggtta tgaatagtaa
     9061 aaataccagt taagcatatt ttcatgtttc tatttttaac attaaatttc caatcttcat
     9121 atgtatattt ttaacaaagg tgtttactta tttaaaaact ctaaaactct ttcattaaaa
     9181 tcctgtgctt cttcatatgt agcgtgtccc aatccctcat agataaatat ttcgcttttt
     9241 ttaatttttt ctactaaatg aaaagaagca ttatttccaa caattttatc attagcacct
     9301 cctataatta aggttggaca tgttatttta tttaattcgg aaaaggcatt atgttctatg
     9361 caagaggtcg cttgaataat aaatcgttta aaatcctttg gtttacctac ttttcctaaa
     9421 aaaggaataa ataattggta tttttttaaa tatctttcag aataggattt ctttgctgtg
     9481 tcaatcatta aatcattgta attctgcttc ttcgccatat taatccaact acaaataaca
     9541 ttttggatag tatcattttg ctttgaagaa gtaactgcca acacaagttt ctcaactaac
     9601 tcaggataat caattgccag atattgagct atcattccac cttgagaaac acccattact
     9661 tctgctttca taattccaag ttttttcatt atatctgctt ggtctttagc catatccctt
     9721 gtggaatatt tttctgtaat ttgatttttt ctactaaata cataaacctt ataatctttt
     9781 gtaaattgct tatatctaaa agcaaaagca atcgcctgta tttttccatg taaaggaaat
     9841 aaaccatctc ctaatcctgg taaaataatt agtgtcttct taccactacc aaattcaata
     9901 taattcactg tattacttcc tatcatcatt tgacaacttt ttgcattata aaacatagtc
     9961 tattcctcgc ttttcctatt ataattgtct aaataaatct ttaaattttt gaataccata
    10021 ctcatcaatt agaggaatat ccattttttt taacatgaat aataaaaaat ccaacctatt
    10081 acatacttca ctgtattcac gtttaaataa tacagggtca agtaaaatat caatactaaa
    10141 aacgagcatt tcagctaatt cctctggata ttcacaagaa aattctttat tttcaacacc
    10201 ttgtttaatt atcttagcaa tattgggtgc aactacattg ttgagattac gaacttcctc
    10261 tattaaagcg aatgggattt tttctaacca gctacaatgt accatttttc tacgtgtgta
    10321 atcattcgca ctatgaacaa ccaattctat tattttttca tttgctgtaa gggtgtcatc
    10381 agcaacaaga tgctctaaaa aattattcat ttgtgagtaa agttcttttg tgacacttct
    10441 cagtatatct tctttagttt taaagtgatg atacattgca cctctagata gaccagataa
    10501 attgataata tcttgtgtag atgttgaatc atatccttta gacgaaaata gtgaaaatgc
    10561 tgattggata attttttctt tactattttt acttttttct tctctattca tatttccctc
    10621 ctaaaaacag actgtctgtt tgtttttcat tatacatttt aatatttatt atgtcaagaa
    10681 ttaactgact ttcaatttct tcatagttta gatggaacga aaagcattcc acatcaaact
    10741 tttaaaaagt tgtattccct caacaaggtc taaaagatac atctctatgc tgtactcgta
    10801 ttccttataa tagagatgta ttgttttcaa agataatcaa gattttgctg tgtttttcat
    10861 acaaaataaa aaggatacct cattagtgct ataacaccta agatatccaa ttaatatttt
    10921 cttaactttt tctaatttac ataaatatag tctactattt taaaactgct cctgtatttg
    10981 ctgatgttac cagtttagca tatctactta agtatccatg ctttattttc ggttctaaag
    11041 gtttaaattt taattttcta ttttctattt caacttcatc tacttttaac tctaattttt
    11101 tgtctggtat gtttatagaa attatatctc cttcttcaac taacccgatt aaaccacctt
    11161 ccatagcttc tggagaaata tggcctatag atgctcctct agttgctcct gaaaaacgac
    11221 catcagtaag aagtgctaca tacttatcaa gtcccattcc tgcaactgct gatgtaggag
    11281 atagcatttc tttcattcct ggaccaccct ttggaccttc atatcttata actataacat
    11341 cacctttgtt tattttttta ccaaaaatag catttacagc ttcttcttct gaattaaaaa
    11401 ctcttgctgg tccttcatga actaacattt cttctgcaac tgctgacttt tttacaacag
    11461 ctccatttag agcaagattt cctttcaata ttgcaagtcc accttggtta ctatatggat
    11521 tctttaatgt atgtattact tcttcatttt ctatttcaca atttcttata ttttccccta
    11581 tagtcttgcc tgttacggtt ttgcaatcta aatttattcc atttatcttt gaaagttcgt
    11641 tcattatagc tggtattcct cctgccatat gtaaatcttc aatatgatgt tttccacttg
    11701 gacttaattt tgttaaacaa ggagtttttt cacttatttc atcaaaaaaa tctaagttaa
    11761 gctctattcc tgattcataa gctattgcag gtaagtgaag tactgtattt gtagagccag
    11821 ccattgccat atccacagct atagcatttt taaaagcatc tattgttaaa atatctctag
    11881 gttttatgtc gttcttaagt aactccataa catacatacc tgcatatttt gctatccttt
    11941 ttctttctcc agaatgtgac gcagcagtac cattatatgg tatacccatg cctaaagctt
    12001 ctgttaaaca attcatgcta tttgctgtga aaagaccaga acatgaacca catccaggac
    12061 aagagttcat ggctaacgct tctaattctt cttcacttac tgttccatct ttacatgctc
    12121 caacaccctc cattgcagag ttaaaatcat atacttttcc atttttttta cctggaagca
    12181 taggccctcc actcacaaca atacttggta tattaagtct tgcagcagcc attaacatac
    12241 ctggcactat cttgtcacaa ttaggtataa gtaccaaagc atcaaaacca tgtgccatag
    12301 ccatagcttc tattgaatct gctattaatt ctcttgaagc cagggaataa ttcattccga
    12361 catgccccat agctatacca tcacataccc ctatagcagg aaattctaat ggcaaaccac
    12421 cactcattct tactccattt tttgcagctt ctgctatttc atcaagatgc agatgaccag
    12481 gaatcacctc attttgtgca tttactatac ctattaatgg tctttcaatt tcttctttag
    12541 taagacccat tccatacatt aacgctctct ttggagctcc ttctattcct ttttttatat
    12601 cacttctcat aatattacct ctttcttact tttttaa
//
LOCUS       DAEOSP010000118        12629 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.119, whole genome shotgun sequence.
ACCESSION   DAEOSP010000118 DAEOSP010000000
VERSION     DAEOSP010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12629
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.119"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            157..1785
                     /gene="aspD"
                     /locus_tag="KRH54_002444"
     CDS             157..1785
                     /gene="aspD"
                     /locus_tag="KRH54_002444"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBG8580099.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1906..2901
                     /locus_tag="KRH54_002445"
     CDS             1906..2901
                     /locus_tag="KRH54_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBG8580100.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3079..3462)
                     /locus_tag="KRH54_002446"
                     /pseudo
     CDS             complement(3079..3462)
                     /locus_tag="KRH54_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      3689..3786
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     repeat_region   3927..4485
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3927..3956
                     /rpt_unit_seq="aatttacattccatatagttaatataaaac"
     gene            complement(4771..4929)
                     /locus_tag="KRH54_002447"
     CDS             complement(4771..4929)
                     /locus_tag="KRH54_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580101.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     gene            complement(5223..6593)
                     /locus_tag="KRH54_002448"
     CDS             complement(5223..6593)
                     /locus_tag="KRH54_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HBG8580102.1"
                     /translation="MKLKKLLSLGLVSMFVAGFPISANALDKIDKIQGADKYETAGII
                     ADKQSYTTAILINADSTMADGLSASGLAGATNAPILLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETFLKDKGIETRRLQGNDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGIESYAIGGTSSMSDSLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSNHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            6809..7926
                     /locus_tag="KRH54_002449"
                     /pseudo
     CDS             6809..7926
                     /locus_tag="KRH54_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861579.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(8289..8369)
                     /locus_tag="KRH54_002450"
                     /pseudo
     CDS             complement(8289..8369)
                     /locus_tag="KRH54_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(8409..9077)
                     /locus_tag="KRH54_002451"
     CDS             complement(8409..9077)
                     /locus_tag="KRH54_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG8580103.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(9930..12350)
                     /locus_tag="KRH54_002452"
     CDS             complement(9930..12350)
                     /locus_tag="KRH54_002452"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBG8580104.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDILDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
BASE COUNT         4354 a         2039 c         1630 g         4606 t
ORIGIN      
        1 ttttaacata tatttaataa ggctaaatag agttattatt ttaatttcat actttacttt
       61 ttctataaac ctattataat taaagtataa tcatttttgc aacttgttta ttttattata
      121 aaaatgtgaa acttattaca taaggaggaa tttaacatga aaaatcatac ttataaagaa
      181 atcaaaaaca tatatggaaa aataagccct tttgaattta aggacaaatt aatagatatt
      241 gcaaaataca ctgcaaaaga aaataacaga gaattacttg atgcaggtcg tggaaatcca
      301 aactggactt gttcaactgc tagagaagcc ttttttactt ttggtcattt cgctattact
      361 gaaactcgtt ctaattggga tttaggtcat ttggctggaa tgcctcaaaa aaagggtatt
      421 aaagaaagat tttttaaatt tataaatgaa aatatagata tgcctggagc atatttagct
      481 agagatatta taaactttgg tataaatgaa cttggatttg atggagatga atttgttcat
      541 gaactagctg atggaattat tggtgataac tatccacttc cagatagaat gttacctcat
      601 atggaaaaaa tagtacacga ttacctagtt caagaaatga aatatgatat atcaggtaaa
      661 tatggtgatg tagaaatttt tgctgttgaa ggtgctacag ctgctatgtg ctatatattt
      721 gattcattaa tggcaaatga acttctaaaa aaaggagata ccatagcctt aatgactcca
      781 atatttacac cttatcttga aatacctaac cttcctcgtt acgactttaa agtggtaaat
      841 ataaatgcta atgaagttga tgaaaaaggt gctcatacat ggcaatatac aaaaaaagaa
      901 ttagagaaac ttcgtgacaa atctattaaa gctttattcg ttgttaatcc taataatcct
      961 gcttctatag ctatggatga aacatcttgt aataatctaa tagatgtaat tgaaaattat
     1021 aataaagatt taatgataat atcagatgat gtatatggaa cttttgtaga agatttttca
     1081 tccttaatgt caaaactacc ttacaacact gttggagtat attcttattc aaaatatttt
     1141 ggagttacag gatggagact tggtacattt gcccttcata agaaaaatgt ttttgataag
     1201 aaaataaatg atttaactgg agaattaaaa aaatcagtag ataagcgtta cagtgatatg
     1261 agtcttaacc cttctagcct ttcatttatg gaaagagttg ttgctgatag cagacttgtt
     1321 gctctcaacc atactgctgg tctttctact ccacagcaag tacaaatggc gtttttctct
     1381 gcttttgcat tgattgacaa agtagatgcc tacaaaaaac tcaatatgag tatctgccat
     1441 agacgtcaaa aactattatt tgaagcttta gagcttccaa ttaatgaaaa caaaaacaat
     1501 gctgcatatt acactcaatt tgatattgaa gaatgggcta aattaaacta cggagaagac
     1561 ttatttaaat ttataagtaa aactgcatct ccagtagatg ttctttataa attagctaat
     1621 gactattcag tagttttact tagtggaaat ggattctatg gtcctgaatg gtcaattaga
     1681 atatctctag caaatctata tgatgaagct tatactaaga taggaaaagc cataagagag
     1741 atattaaatg gttatatcgt tgaatggcaa aaaattaaga aataattata ttacactgaa
     1801 atatctaaat tatatgaata aaagagttta tgtagacctt aactactttt tttgatataa
     1861 tcctatcgga attataaatt ttatttatga aaggcagaga atagtatgag tattaagaaa
     1921 aaagttgcca tagtttttac tggtggaact atatctatga cagtagatga taaagtcgga
     1981 gcagctatac caactttatc aggtgaacaa atactatcaa tggttaccaa cattgataaa
     2041 gttgcagatg ttgaagtttt taattttgat gaaattccag gacctcacat aacaccttat
     2101 agaatgatgg aattgaaaaa ttatgtaaat gatttattag caagaaaaga tattacaggt
     2161 gttgtaataa cacatggtac agatagttta gaggaaacag cttattttct agaccttaca
     2221 attgacaaca tcaaacctgt aatagtcaca ggtgctatga gaagtagttc tgaacttgga
     2281 tatgatggtt caagtaattt atctgcatca gtttgtactg cagtatccaa agatgctatg
     2341 ggaaaaggag tccttgtagt tttaaacaat gaagtattat tagcttctga agctactaaa
     2401 acaaatactc tttctctaaa tacatttaaa tctctaacta gtggcccatt aggaataatt
     2461 gactgcaacg aacttgtcct tacaagagat attgtaaata gaacaattat agatactgat
     2521 aaggtagaat ctaaagttgc cttatttaaa gcatatgtag gagaaaatgc tgactttata
     2581 agatttgctg ttgatagtgg ttataaaggt atagttattg aggcaatggg tagaggtaat
     2641 attcctcctc aaatgctttc tggagttgaa tatgctagag aaaaaggact tcctgtagtt
     2701 atagtttcta gatgtcattc aggtagagta ttcgatagtt atggttactt tggttctggt
     2761 agagatttaa aaaatcttgg ttgtatattt ggtggggact taccaggtca aaaagctaga
     2821 ataaaactta tcttagcact tggaaaaact gataaccttg atgaaatcaa agatttcttt
     2881 gaaaaaggaa tctattgtta attctaataa cttctattta attatttatt gatatttcaa
     2941 catttttaaa tcaaaatcta atacttatgt cagttaaatg tcagcaaaat attttatcca
     3001 caagttttcc acaacttatc cccaatatta tccacaagaa aaaaggtagc acttatatta
     3061 gtagctacct tttatttatt aatcttcatc tttccagctt tttatccaat ttcctatttc
     3121 tattattttt tcttcagtta tttcttctcc atcatgcaat tttgaaatta attctgataa
     3181 tggattgtta tgtagacctc caaaaattct ttttccctct attttgagat attctttctc
     3241 ttttattaat actctatagt gtacatgttt cccaattctc tgcgatatga aaaaaccttt
     3301 tttagttaat ctagctaaaa gtgtaattgt agttgtatgc ttccaagcat atgttttttc
     3361 tatttcatct gcaacatctt ttgatattac agttttataa ccagtattct atatgtattt
     3421 cataattttt aattctgcat ctggtaattt tttattcaac ataatatatt ccttctttca
     3481 atttatgtca cttcctcaaa cttatctagc ttaaattaat aataggagct ttttatcaca
     3541 aaaactacag gtaaattttt ctatatattc tacgtattta gtctgttttt taagtgtgaa
     3601 attacgttca tacctataaa tttattcaat tttcaaacaa tcttattgaa taataactaa
     3661 ttctattgta ttatttagtt atagaataaa actaaatcgg caaaactaga gaaatctagt
     3721 gacgcaaagc tatagagact aagacttata taaatatctt atgagttatg tcagccagtt
     3781 gccaaagaga tattgttctt tttgttttta tgaaagtttt ttagggggat aataatttat
     3841 atatttagag ataaaataga taaatgtaca catatattga ctgcttatat tagcagttcg
     3901 tatgattatt gtaatttttt ggatacaatt tacattccat atagttaata taaaacaagt
     3961 gtttcgaatt tcttttctga aactatacca acaatttaca ttccatatag ttaatataaa
     4021 acgttatata ctgaaagcgg tgaaactggt tacgtagcaa tttacattcc atatagttaa
     4081 tataaaacct gtgcgtgtag gagatggtaa cacaactgtt gaagaattta cattccatat
     4141 agttaatata aaacacacat ctacatgttt ttctatctga gttcatctct aatttacatt
     4201 ccatatagtt aatataaaac tttttaatcc ttcttctgaa ctcccaaagt ttatatcatt
     4261 tacattccat atagttaata taaaacccac gaaatttaag gtcgatatat cagatttgaa
     4321 aaaatttaca ttccatatag ttaatataaa acaagctaaa tggagcaacc gaccaagtcg
     4381 ctagaaataa tttacattcc atatagttaa tataaaactt ttctattaag tgtaagcaga
     4441 atgcatggaa tatgaaattt acattccata tagttaatat aaaaccccaa aataaattga
     4501 gtatttccaa tgcctacaca tatacaaatc tctcaaattt gcagtgaacc atgagtagtg
     4561 caattgataa cacttatcac aagtcctcaa tgccttatat ttcaactgtt aagccttaat
     4621 ttcttcaaaa aatcgaacac tgcaaaattt ctatgacttt attatatcat aaaaatatta
     4681 tttttgaata tctgtaccaa tttgtgatat aataaaagca agaagaacta caatctattt
     4741 tgctgtagag tggagttcat aatttagaaa ttacttagtt ttatggaatc tgatttttaa
     4801 atcaaattcc caaccactct tagttgccgc tttgagtggt tttttacgct ttctaaatag
     4861 tttgctagct atataaacta ttaagctagc agatagactt gctagtatgc cttgtaaaaa
     4921 attatccatg tatattcacc tcccttctta acgtcgggag gataattttt tgatacatga
     4981 actccactct ataaattgta gattacatct tcttgctaaa aatattataa catataattc
     5041 ttacatattt tacctattta ttacttttta cattataata atattctttt aatcaaaaaa
     5101 acacctacta ttaaagtaaa tgtttttaag tatttttaat tttaaaatcc atatagttca
     5161 tatgaaacta aatataataa ctcaacatat gagcaatatc gagacattta catacccact
     5221 agttatttat attcaaaaaa atcttctcca tccgggttat aaaataatat tctacttgcc
     5281 aatccttgtt catatttata attttcttta tcacctacat aagaaaatgt taatgtcatg
     5341 cttcttgatg gatctaatga aattatatct aaataaatag tacatttttc tcctggcttt
     5401 aatacaccga aactattaaa atccattttt attttagcgt attgccccat atataatgtt
     5461 gaattagctt ctccataaac ttttaatttt gttacatcta aattttgtgt acttgtattt
     5521 tctatattta taggaactgt tgggtcttta tatttttcag tataatattc aaggttatca
     5581 tcataacatt caagataact tcctttatct tgccaaatta tgtgatttga atcaaaatca
     5641 atctttaatc cacatattat atttttattt gaagatggaa gactaatatt attgatggta
     5701 ttttcacact ctagcataat tttatcactt agatttccta tcccggttac ttttgcagca
     5761 tttttaagta tatctttatt acttccaata tcaactagta caataggatt atttttagcc
     5821 attggtgaag caactaaagc ataaactaaa tcagtaccac tagctatata aaattctttt
     5881 gcgccattat agaacttatt tactattttt ttattagtat catatctatc tactccaccc
     5941 aatcgagttg aattagtatc atttactaaa ctatcactca ttgatgaagt gccacctatt
     6001 gcataacttt ctataccagt tgtattaaat ggaacacttt ttccatcagt taatactata
     6061 gctgctttat ctctaactgc tacaggtgca gcactcatag catctggctc acctttgaaa
     6121 gcgttagtta atattacttt atttacttta ttaatggaat ttatttcttt tgctacatta
     6181 taacttgtct taatcctatc attaccttga agccttctag tctctattcc tttatctttt
     6241 agaaaagttt ctgttgcttt atcaatggaa ctttctcctc ctattatgta taccttttta
     6301 gctttttcta ctcttttaag agttgcatta ggtatattat tctttttagt caaaaggatt
     6361 ggtgcatttg ttgctcctgc aagtccactc gcacttagcc catcagccat tgtactatct
     6421 gcatttataa gtattgcagt agtataactt tgtttgtcag ctatgatacc agcagtttcg
     6481 tatttatctg ctccttgaat tttatcaatt ttatcaagtg cattagctga tataggaaaa
     6541 ccagcaacaa acattgacac taaccctagt gataataatt tctttaattt cacaatatct
     6601 ccccctttta tatatgaaat actatatatc ttaataattc tacattaaat tttcttttcc
     6661 tcctttaacg atatttatct tcctgttaat ttaagtgtat acaaatacgt gttataatgt
     6721 aactgtaaag aattaaaaag ataatgacat aattagagta gtacaactgt tcttttataa
     6781 aactaactta taaagggagc gtacatatat gaatatcaaa tcagctttta taagaaaaag
     6841 aggggaaaaa ttctatgtat atgtggagta catagaggaa acaactggca agataaaaca
     6901 gaaaagttat ggaagttatg aaaagaaaaa ggatgctgaa aaacatttaa ttgaaataaa
     6961 atctactata aacaataata agtttgttgc tccaaaagat atgacatttg tagaaagatg
     7021 ttataaatac atggacatga ataaaaataa ttggtctcca tatactgtta caaatacaaa
     7081 atcttgggtt aaaaattaca tagaacctct ttttaagaat ctaaaactca cagaagtaaa
     7141 tcctagttca gtacaagctt ttgtaaatta taactttaat aattctacct ttactagtgc
     7201 taaagtaagg tatagctttc taagttctgt aataagggaa gcatatagat taagagaaat
     7261 acaagaaaat tcatgtgatt ttataaaact tccttctaaa gaaaaatatt ctacaaatga
     7321 aatatacaat agaaaagaag tattattgct aatagaaaag ttacttagta ataatataga
     7381 aatacctatt cttttaatgt tacttctagg tttaagaatg ggagaagcta ctgggcttag
     7441 gtggtcagat gttgacttag ataacaatct gataaatgtt aatcaaattc ttgtctattc
     7501 taatggcaaa atatctttta aagagccaaa aacaccaaaa tcaaaaagag ctttatcagc
     7561 tccaaaagaa ttaattgaaa aattgaaaat taaaaaacta agacaaagca aattaaaatt
     7621 gtaaggtatc ctggaaaaca gaaataattt agtttgtcta aacaccaatt taaatccgtt
     7681 tctaccttct tcactaagaa aaacgtttca caattttaca aaaaaaataa tttaaaaaag
     7741 attagaatac atgatttaag atatacaagt gccactctgc tgcttttagg gggaactaat
     7801 atgaaagttg tttctgaaag attgggtcat acagatataa aaataactat gaatagatat
     7861 tctcatgtac ttaaagaaat ggacaaagaa gcttccgaca atctaagcga acttctattt
     7921 aaatagtaaa aactgactgt cagttaaatg tcagttaaac ctaaaaaaat aggttttgtc
     7981 agttgattat cagtcaatta ttttataata gtttcactta aaaacgtcta tatctacaca
     8041 ttttactatt tgtttatgct tattaatatc tatctgttat tattaattct aatagcttct
     8101 atttaattat ttagttcagt ttacaggaaa ctacacacaa accataaaat aggatgttga
     8161 caatataaca gatagtatgc tcctgtaaaa ggtaacgtac tatctgttat gttgtctgga
     8221 tttccaaatg gttacccttt ggcacacaac tttgctatct ccttggtaaa ctaaaagttt
     8281 gtaatctaaa atcctaatcc atctctataa aatttaattg acttctccat actttttaca
     8341 cctaaacata taaaagtaat tttattcact taatgacctc cataattccc aaattagcta
     8401 tttggaactt aactaaaaaa tcctaatata agggcaacta ttataatggc aatcggaata
     8461 catactgtgt aatcctgttc catagagtca tcatctgaat gtagttttac aattcgcaat
     8521 ttagcaatca gtttgaagca gaaatagcct attattgttc caattacatt cgtgagtaag
     8581 tcgtctatat cggttgctct gtgtaacgta aaaagctgaa tagtttcgat aaaaagagat
     8641 aatccaaaac caatcaagaa tgtatttctc aaacgttcaa aggttttgct gataagcgga
     8701 cataaaaaac ctaatgggac aaaaagtaaa atattcaaaa taaaactcaa actaaatcca
     8761 tcactaaaag gaataagatt tatgtttgga ttaaagaacg tttcccctaa ctgtgacagt
     8821 cttatatatt cactgaatgt agggatacca acaatatgcg atagtgttaa acataagtag
     8881 taatacattg ccagtgataa aatagatgtt tttacctttg gaatatcact atttttcttg
     8941 aatattacaa ctaagaaaat accaatcaag ataattccca acaagaaaat aggattaatt
     9001 aacaataaat ctttgatagc ttcaaacaga ctgagttgaa tttctacacc tgattgatta
     9061 tttgttacag tctccatttg tacatacctc ctcataacat ttcaattatt tctaaaacac
     9121 aaaatagaag ataaatctta aaatcatcct atcaaaattt ttaaatttgc ctttatcagt
     9181 ttataccacc agttaattcc tgtcttactt ctgtatggta ttcttgggat tctttcatca
     9241 aattagacaa aaaatggtta ccatatcaat aaactggtta cgctccatat ctcccgaagc
     9301 aaccatcccc tgagaataaa tccgtatcag gaatctccac atctactgca cctatcacaa
     9361 gttatttgat aacatcctca gacaatccca acttggatat atcgacaaca cccattagaa
     9421 taccactgct gaccatactt gttttcctgc tgataatttt aatcaactgg gaagttgaaa
     9481 cgctatcttt tttacaagtt cctttttaga aacatggtag ctattatatg aatctgaatt
     9541 tcgttgtctg ggtgtttcat ataaagcata gatacgcacc ttttttgcct gttttcatta
     9601 tatagaaaaa aatttcaaaa tacaataatt atattttttt cgtttaagct tagagcagtt
     9661 ccttgatgaa caacactttg gaaactgaat gatttacata ccacttaata cattaactcg
     9721 atttaacatt aattataata atatttaaaa caacaactag ggtatataag aatgaacttt
     9781 ttagttcttt aatcattttt ttgtataaaa tcaaatctat ttttataatt tcaacaaatt
     9841 tataatttca acaatgaaaa aaggcttatc tctattttga gacagcctcg tttattttat
     9901 aaatttatat ttttgttcat atttttgagt tatttaacaa ctatatttac tagtttttta
     9961 ggtacagcta caactttaac tatagtttta ccttcaacta atccttttat tttttcatct
    10021 tctaaagcta ccttttccat ctcatcttta gatatatttg agttaacagt taattttcct
    10081 ctaacttttc cattaacttg tacaacaact tctatttcat cttttactaa agctttttcg
    10141 tcatatttag gccagtctat gtcaaataca cttccttctt tacctatcat agtccatagt
    10201 tcttcaccta aatgaggggc aaatggtgca attatagtta ctatagtttg tacaccttct
    10261 tttataactg cttcgtttat attatctaac tctttatatt tatacatatc atttattaat
    10321 tccataagag ctgatatagc agtattaaat ccgaattttt cacttaaatc agcagtaact
    10381 tttttaagtg tagagtgtat agtatatctc atatccttat cttgggaatt taattctcca
    10441 aactctacat cctttttaac cacatctgct aactcgtcaa ccaatctata aaccctattt
    10501 aagaatctaa agcatccatc aacaccttgc tctgaccagt ccaaatctct ttctggtggt
    10561 gctgcaaata atacgaaaag tcttgcagta tctgctccat actcatctat tatatccata
    10621 ggagatactg tattcccttt agacttactc attttagaac catccataag taccattcct
    10681 tgagtaagta agtttttaaa tggctcattg aagtctacca ttcccatact cttaaatgcc
    10741 tttacaaacc atcttgcata aagtaaatgc attatagcat gttctactcc acctatatat
    10801 tggtctactg gatgccatct attaactaat tccttactaa atggttcatt ttcattattg
    10861 ctatccacat atcttaagaa ataccaagaa gaatcaacga atgtatccat agtatcaact
    10921 tcccttctag ctggttttcc acagtgagga caagttgttg acataaattg ctttgaagtc
    10981 gtaagtggag attccccttt tccagtaaat tcaacatctg ttggtaaaag aactggtaaa
    11041 tcttcctttt taactggaac tattccacat tcatcacaat aaaccacagg tataggacaa
    11101 ccccagtatc tttgtcttga aactaaccag tctctaagtc tataattaat tgttctcttt
    11161 cctctacctt ctttttctaa cttatcgatt attccttcaa atccttctga agcatctaat
    11221 ccattaaatt cttctgaatt tatcattttg ttatcttctg ttattacatc aattatgtct
    11281 aaattatatt tttcagcaaa atctctatct ctttcatcat gtgctggaac tgccattata
    11341 gcacctgttc catactcaac taatacatag ttagctatcc atagaggaac tttcttgcca
    11401 tttaaaggat ttataacata tctacctata aacattcctt ctttttctac atcagctgct
    11461 gttctttcta tttctgtcat agtatgcatt ttttgaacaa aagcttcaac atctgcttca
    11521 tattcagtcc ctgcaaccaa ttctttaact aattcatgct ctggagcaag taccatataa
    11581 gtaactccat aagttgtgtc tggtctagtt gtaaatactg tcatagattt atcagttcca
    11641 tctatatcaa aaactaaatc tgctcctgta cttttaccta tccaattttt ttgcattgtt
    11701 ttaacttttt ctggccatcc atctaatata tctaagtcat taagtaattc ttctgcaaat
    11761 tcagtagtct taaaatacca ttgttctaaa tctttcttta atactgttgc tttacatctc
    11821 tcacatgctc cttgaacaac ttgctcattt gcaagaacag tctcacaaga aggacaccag
    11881 ttaacatatg attttttctt atatgcaagc ccatgctcta aaaacttcaa aaatatttct
    11941 tgtgtaaatc tatagtattc tggcgttgaa gttgcaactt ctttatccca atcataacta
    12001 atacctaata aatttaattg ctctttcatt tcttctatat tttccattgt ccatttatgt
    12061 ggatgtattc catgttttat agctgcattt tcagctggta atccaaaaga atcccatccc
    12121 attggatgaa gtacattata tccttccatt tttttgaatc ttgcgacaac atcacctata
    12181 gaataatttc taacatgtcc catgtgtatt tttccagatg gataagggaa catttctaat
    12241 gtgtaataat taggtttttc agtttgtgca gtatccattt tatactgatt attatctttc
    12301 cagatttttt gccatttcga ttctacttct ttaaaattat atacgctcat attttgcctc
    12361 ctatttttaa ttgtgtataa ctaccttatt tagtattatt tacttataat ttccaaataa
    12421 ttctttgaaa ataaaaaatc ctcgtcacta aacttacgtt tagggacgag gataaatctt
    12481 cgcggtacca ccctaattag cctactacat atatagactc tcttagatag cttttaatcc
    12541 agccatggag aatagatttt aatctagtaa gttcaaagtt atataatatt agttctcacc
    12601 ttccactaac tctctgaaaa aatataatc
//
LOCUS       DAEOSP010000119        12503 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.120, whole genome shotgun sequence.
ACCESSION   DAEOSP010000119 DAEOSP010000000
VERSION     DAEOSP010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12503
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.120"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..369
                     /locus_tag="KRH54_002453"
     CDS             <1..369
                     /locus_tag="KRH54_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580105.1"
                     /translation="NKNEELKNNFNNSFLDKKTNKDIVLLVENELRDIVNVEYINQES
                     SLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEINKLKVNNFDVQNIP
                     NEYKQEKDNIVECKMTVRVI"
     gene            622..1659
                     /locus_tag="KRH54_002454"
     CDS             622..1659
                     /locus_tag="KRH54_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBG8580106.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGTNLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            1740..2105
                     /locus_tag="KRH54_002455"
     CDS             1740..2105
                     /locus_tag="KRH54_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580107.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            2110..2427
                     /locus_tag="KRH54_002456"
     CDS             2110..2427
                     /locus_tag="KRH54_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580108.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            2475..4079
                     /locus_tag="KRH54_002457"
     CDS             2475..4079
                     /locus_tag="KRH54_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580109.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNSLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            4203..5831
                     /locus_tag="KRH54_002458"
     CDS             4203..5831
                     /locus_tag="KRH54_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG8580110.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            6096..7445
                     /locus_tag="KRH54_002459"
     CDS             6096..7445
                     /locus_tag="KRH54_002459"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBG8580111.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            7601..8680
                     /locus_tag="KRH54_002460"
     CDS             7601..8680
                     /locus_tag="KRH54_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG8580112.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            9025..9933
                     /locus_tag="KRH54_002461"
     CDS             9025..9933
                     /locus_tag="KRH54_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBG8580113.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            9938..12307
                     /locus_tag="KRH54_002462"
     CDS             9938..12307
                     /locus_tag="KRH54_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBG8580114.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
BASE COUNT         5100 a         1315 c         2319 g         3769 t
ORIGIN      
        1 aataaaaatg aagaattgaa aaataatttt aataactcat ttttagataa aaaaacaaat
       61 aaagatatag tgttattagt agaaaatgaa ctgagagata ttgtgaatgt agaatatatt
      121 aatcaagaaa gtagtttaga taatcacaat aaaaaacaag ttaatttaga aataaaagtt
      181 aatggagaac ttgcaaatgt gttgaaactt gaaggtgtaa tgaagaagtt aagactaaat
      241 aataaacttg aaaagataga aattaataaa ttaaaagtca ataattttga tgttcaaaat
      301 attccaaatg aatataaaca agaaaaagac aatattgttg aatgtaaaat gacagttaga
      361 gtcatataac aaaaaagaat agataaagta aaaaactatt atcaattaat aaaattttat
      421 agaggaatta gttttacgat aattcatttc tacaagaggg attgttgatg attttatcga
      481 aatcctaatt aaagttaata tattgtacca aaatataagt gaatttaata gaatatttac
      541 ttatactata gattgattaa taaaaaaatg gtataataac aaattgtact ttaatttaat
      601 aataagggag tggaagtcat attgttaggg agaaatgagc tatgcccttg tggaagtggc
      661 aaaaaataca agaaatgttg tttaaataaa gatattgttt cagaaagagc atgcagaaaa
      721 actgaacttt cacaaaaaca atatacagat ttatattcaa agttatatga ttattcaaga
      781 caggataagt ttaaagaaga atgtgaaaaa gctaaagaaa tgttttatat aatgcaaaat
      841 gatagtgtaa atgaaaaatt tgaaagattt tttaatactt atttaataca agaccacata
      901 atggaaaata aaaaggttat gacagttggt tttcttgaag aaaatggtac aaacctaagt
      961 caatcagaag tatcaatatt aaaaagtatg tttgaatcat acgtaagtat ttatgaggta
     1021 aaagaagttt ctgctggaaa aatagtatta aaggactgtt taagtggaaa tgaactttgt
     1081 acagaagatg taaaacttct gagaagtttt aaaatcggaa gttgtatgat agctagaata
     1141 gttgatattg aaggaactaa catattgata gatataacta taagtatatc aaatgaggtt
     1201 aaagatataa ttttaaagga tattatgaat ctattcaatc aatatcaaga tgtatacaaa
     1261 gatatgaagt cgttcttaat ttatcataca catatactat acaagtatat acaacagctt
     1321 ttagacccaa gtatagctga atatttaaag agagaaagag ataaaaacga tacaaaagtt
     1381 aattcagaaa atgaattagc tgaagaagaa tgtaaagttt tagatactct taaagaaaat
     1441 gtagaaaaag aaaatataga tgcatgtata gagttttgga atgaatataa gaaaaatcat
     1501 gaaaatataa aaggagctga aaatggttgg gcagctgctg ttgaatacta tgttaaaaaa
     1561 gaagcaggac aacctataac tcaagttcaa atatcaaaga aatacgatat aagctctagt
     1621 acactaggaa agagatacaa ggatttaaaa atatcttaat taattataaa aagctttaag
     1681 tcatagataa agaatctata gtataatata tgtataatgt ttagaaagag gtattaataa
     1741 tgagtcttag tttaggaaat gcaattgctg tatcaacgaa ctttataaaa tctaacccag
     1801 ctgtacctgt gtacaacgga gaagaaatat atgcagttat ttatcatata gaaataggat
     1861 ataataatgg tgtatcacct gataccaaac aatatataat aatagactat aaagagtttg
     1921 atttatctat ggataaaaaa aatgaactaa taaatattgc taaagagtac tgtaaatcta
     1981 aaggtattgt agaaaacgaa aaccttgaaa taaacctttt agacaatgaa gaagcagaga
     2041 acttcttaga aaaggtgttt aataatatga aggttataag aggtcttata actaaaaaag
     2101 gataaataaa tggactatat aggaatagaa aatataacac cttatgaaaa tacatatgaa
     2161 tttagtgtat atgaatatga tgatgaaatc accttaggta gtgaaaagtt atatgtatgt
     2221 gaattgaggg ttgtattgat taaagttaat tctctgtatg ttgaaagatt gcataaatca
     2281 gttgaagcaa tggtcttagt aaaaaacttg aaaaaagatt tagataaaac acttgttgta
     2341 aacaaaataa agaattttgt gctagatgag atttgggtag aaaatctagt aaaagagaat
     2401 atagaagtta tatttgtaga aagctaggaa taaccctagc ttttttatat tacaatctaa
     2461 gagaggaaaa caatatgtta agagtatcta atgtaaaact tggaattgat aaagatatat
     2521 ctttattaaa agatgttata cttaagaagc taaaaataaa agaaaaaaac ttagtcaaat
     2581 atagtatata taaagaatct atagatgcta gaaaaaaagg aaaaatggaa tttgtttatt
     2641 ctgtagatgt agaagttaaa gatgaaaata aaatacttaa ctcaaaaata aaagatgtaa
     2701 caaaaataaa ggaaatcaga tacgtaaatg tacctgtggg tgataaaaaa ttaaagaata
     2761 atcctttggt aattggtagt ggaccagctg gtttgtttgc aggtctgctt ttagctcaaa
     2821 tgggttatga accaatcata cttgaaagag gtcttgatgt agacagtaga accaaagata
     2881 taagtgattt ttggacaact agaaaattaa aaaataattc aaacgttcaa tttggagaag
     2941 gtggagcagg aactttttcg gatggtaaac ttacaactag aataaaagat atacgatgta
     3001 gaaaagtatt agaagagtta gttcagtttg gagcaccaga tgaaatatta tactcacata
     3061 agccacatgt tggaacagat atattaaaaa atgtagttaa aaatataaga aatgaaataa
     3121 taagtttagg tggtcaagtt agatttaatt caaaagtaac agatatagtt gtatcaaatg
     3181 attctataca atctgtaata ataaatgata ctgaaaaaat agatacagat acaattatat
     3241 tagctgttgg acatagtgca agagatactt atgaaatgtt gtattcaaga ggtataaaaa
     3301 tagttcaaaa accttttgca ataggagcta gagttgaaca tccacaagat ttaataaata
     3361 aagcacagta caaggatttt tataatcatc ctaggttagg agctgcagat tatagactta
     3421 tagagcatgt ttcaaatgga agaacagcat atactttttg tatgtgtcca ggaggtagtg
     3481 tcatagcatc tgcatcaaat gattttgaaa tagttacaaa tggtatgagt gaacatgcaa
     3541 gagataaaat aaatgcaaat agtgcatttt tagtaaatgt aacgccagaa gattttaaga
     3601 gtgataattc actagcaggt gttcatttcc agcagaagta tgaaaaatta gcttttgaat
     3661 taggtggaaa aaattataat gcaccagttc agcttgttgg agattttata aacaataaga
     3721 cttctttgaa tttaggaagt gttgagccat catatagacc aggatataca cttacaaatt
     3781 taagtgaatg tttgcctagc ttcgtaacag agactatgag agaagcgttt atatctcttg
     3841 ataaaaaatt gaatgggttt gcaatgtatg atgctgtttt gacgggagta gaaactaggt
     3901 catcatcacc aataagaata gtaagagatg aaaatacttt agaatcagtg agtattaaga
     3961 atctatatcc atgtggtgaa ggtgctggat atgcaggtgg tatagttaca gcagcagttg
     4021 atggtataaa atgtgcagaa aaaataatac aaaaatacac caataataca aaaatttaac
     4081 ataaacttaa tgatttttta atatttaaca tgaatttaac attatcactc ctataatttg
     4141 ataaaataca ataggttata aaatttttag atacagtaat ctctttacaa ggaggaaata
     4201 atttgaacat tataataagt ctaatgggtg gattaggtct gttcttatat ggtatgaacc
     4261 ttatgggaga aggtcttcaa aaatctgctg ggacaaagct aaaaaagata ataaaactac
     4321 ttactagtaa cctatttatg ggagttttgg taggtacagg agttacagca gtaatacaaa
     4381 gttcaagtgc tactactgta atggttgttg gtttcgtaaa tgcaggaata atgactttaa
     4441 aacaggcaat aggagttata atgggtgcta atatagggac tactgttaca gcccaattgg
     4501 tatctttcga cttaactggt atggccccag tagcattagg tgtaggtatt atactttatt
     4561 tatttggaaa taagcctaga ataaaaaata tagccgaaat acttataggt tttggtatat
     4621 tatttacagg tatggatttt atgaagatgg cagtggagcc actaagagac tatcaaggat
     4681 ttactgactt acttgttaca tttggtagat atccactact tggattatta ttgggatttg
     4741 ggattacagc aataatacaa agttcaagtg catctatggg tatgttggtt gcactggcag
     4801 cagaaggtct agtgccatta tcagcagcac tgccaattct ttatggccaa aatattggta
     4861 catgtgtaac ttcactgtta tcaagtattg gtgctaacaa aaatgcacga agagcagcta
     4921 tgatgcatct aatcttcaat gttttaggaa cagtgatatt cttaatattc ttgaataaac
     4981 cagtagtaag catggttaca agttgggacc caagcaatgt tgcaagacaa atagccaata
     5041 cacatacatt gtttaatata atatctgtat tgatactttt accatttaca aatcttatta
     5101 taaaactagc tataaagctt gttcctgata gagcaggtga tatagatgaa gatgaaacta
     5161 aaactattaa atatatagat gatagaatga tagaaactcc ttctatagct cttgcaaata
     5221 cgataaaaga agcacttcgt atgggtgaga aggcgaagga aagcctaaat gcatctatgg
     5281 aagctttagt agaacattca actgaaaaaa tagataagac atataggcga gagagattaa
     5341 tcaatgattt acagaaagct attttaaatt atttacttaa actttcaaaa gcacctttag
     5401 atgatgattc aagagaagtt gtggatactt tgtttaatac agtcaatgat atagaaagaa
     5461 ttggtgacca cgctaaaaat atagctgaat tatcgcaagt tgcaatagat tcaaatattt
     5521 ctttctcaga agaaggacaa tctgaacttg atgttatgta taatagagtt gtttcagcat
     5581 atacttatgc attagaatca atgagaactg ataatgttga tttagcttgt aaggttataa
     5641 aaattgaaga acaagttgat ataatggaga aatcttgtag agctaatcac atgtatagat
     5701 tgaacaataa tctatgtagt atagaaaatg gagttattta tttagacgta ataagtaatt
     5761 tagagagaat atctgaccat gctgtcaata tagcacaaca agttatagca aagagattag
     5821 gaaatgatta aaaataatta atttaaaata atataaaatt taaaatagag gataaataaa
     5881 agagaaactt taaatgatac gtttagagtt tctttttttg taaatttatt tgactaaaat
     5941 ttttaaattt atacatagtt atagcattta gaagaatcga tttcctgata ataaatgatg
     6001 agatatgtat gattttgtgg caaactttta aaaaaaacac ctatattttg tgttataatc
     6061 tctttaaaac gaatatgctg aaaagaggga tttaaatggg aaaaataaat tttgattata
     6121 gtaaagctac agagtttttt tgtcaaaacg aaatagatgt catgcaacca tatgtagatg
     6181 ttgcgcacga catgttacat aataaaactg gtttaggaaa tactttttta ggatggatag
     6241 accttcctaa aaattatgat aaagaagagt ttgacagaat aaaaaaatct gctgagaaga
     6301 taaaatcaga ctcagatgtt cttttggtaa taggtatagg aggctcttat ttaggttcga
     6361 gagctgctat agatatggta agtcattctt ttaggaatgg acttaaaaaa gagcaaagaa
     6421 aggctccaga agtatatttt gtagggcata atataagctc aacatatatt atggatttat
     6481 tagatataat tgaaggaaag gatatttctg taaatgtaat atctaaatca ggaactacta
     6541 cagaaccagc tctggctttt agaatattta aagattactt agagaaaaag tatggtaagg
     6601 aagaagcaag aaagagaatt tatgcaacaa cagatgcaag taaaggtgca cttagacagt
     6661 tagctacaga agaaggatat gagacttttg taataccaga tgatgtaggt ggtcgttttt
     6721 ctgtattgac agcagttggg ttacttccta ttgctgctgc tggacttgat attgatgcta
     6781 tgatgaaagg cgcaaatgat gctagagaag catttcaaaa tcctgactta aaaagtaatg
     6841 atagttatag atatgctgtt gctagaacaa ttttacatag aaaaggaaaa gatgtagagt
     6901 tattagtgaa ctatgaacct caactacatt atgtaagtga gtggtggaaa cagctttatg
     6961 gagaaagtga aggaaaagaa aataaaggtt tattccctgc ttctgtagat ttctcaacag
     7021 atttacattc tatgggtcaa tacatacaag atggaaaaag gcttttattt gagacagtac
     7081 ttaatgttga aaactgtaag agaaatataa ctataagttc agaggaagta gacttagatg
     7141 gtttaaacta tttagcaggt aagactgtag attttgtaaa tcataaagct tttgaaggaa
     7201 ctctattggc tcatacagat ggaaaagtac ctaatttagt aataaatata cctcaactag
     7261 atgagtataa ttttggatat ttagtttatt tctttgagaa ggcttgtgga ataagtggat
     7321 atttattggg agttaatcca tttgaccaac caggagtaga agcttacaag aaaaatatgt
     7381 ttgctttact aggcaaacca ggttatgaaa aagaaaagga agagctagaa aaaagactta
     7441 aataatttta aatagttaag gtttaattaa gattaaaata atataatgaa atcatcaaaa
     7501 aggagtttac tcaaaataga tgatttcatt ttttttatga aaaattggga aaaatatatt
     7561 ttgataattt atattatatt aagataggga gtggtatgag atgtcaagaa gaaagaaagg
     7621 gataagttta gtaatattag ttgctataat aagttcaata cttagtagtt ttctaacaat
     7681 aattttagtt aaggacaatt tagtaagcaa atctacagga agttctacgc cgatagttgt
     7741 aaatgatgat ggtaagagtc aaaatattta tcaagcagtt gcagaaaaag ctacgccatc
     7801 tgttgtaggt attacaacaa ctagtgttga tactagtaat atgtttgcaa taccaactga
     7861 aacacaaggt gtaggaactg gaatcatagt tgattcaaat ggatatatac ttactaattc
     7921 tcatgttata tcagatggtc aagctacgag tgtaaatgta ttattcaatg atggttcaac
     7981 aacttcaggt aaagtagtat ggtttgacca acaacttgat ttagctatag ttaaggttga
     8041 taaaactgga ctaacaccag cagaatttgc tgatagtgac aaagtaaaag ttggagatat
     8101 atcaatagca ataggtaatc ctcttggatt agattttcaa aaaacagtta ctcaagggat
     8161 aataagtgga ttagatagaa ctatacaaac agaaaaaact aatatgactg gtttattaca
     8221 aactgatgca agtataaatg caggaaatag tggaggacca ttgttaaatc aaaaaggtca
     8281 agtaattggt ataaacacag caaaagcttc tcaagcagaa ggattaggat ttgcaatacc
     8341 aatcaataca gctaagagta tagttgaaga agtaatcaaa aatggaaaat atgaaaaagt
     8401 aactttaggc ataaaaggta cagatgtatc aaattatgaa gcagcaacag gtacaaaatt
     8461 aagtactgat aaaggtgttt atgttgcaga agtaatatct ggttcatcag cagaaaaagc
     8521 tggagtaaag gtaggggata ttataactaa agtaggagat acagatataa caggtatgaa
     8581 tgatttaaat aaaaagttgt atactttctc aaaaggtgct agtacaaaaa taacagtaaa
     8641 tagaggcgga aaagcagtaa caataaatgt gaatttctaa atatgaccag aaagatagag
     8701 actacatggt aaagtgtagt ctctattttt atttaatttt tatagtatat acatctaaat
     8761 taattaaaat gatgtattgt ttatagctta ttgctatatt ttcttataaa aataaattta
     8821 ataaatattt aaatttaatt tacaaaaaca atataaatat cataataaaa taaaattttt
     8881 taatattact aaaaatatat agacaaaata ataacaaata tagtattatg taattgtaga
     8941 aatgcttttc ataggtatat tctatgaaga tgaattgcat aaaaatgaaa aaatggaatg
     9001 gatattattt aggggtgaaa aatcatgaat ccattagtta taaacttaca aaaatgtagc
     9061 atacatgatg gaccagggat aagaagtaca gtatttttta aagggtgtcc acttgaatgt
     9121 gtttggtgtc ataatccaga gagtcaaaca tatacaaaac aagttttgta taatgaagaa
     9181 agatgttcaa aatgtgaagc atgtataaat atatgccctc ataaggcaat ttacaaaggt
     9241 gaaacaaaaa tatgcttaga ccaagataag tgtgagtttt gtgaaacttg cttagactat
     9301 tgtgtaaaca atgcaagaga gattgttggt caagagtatt ctgtaagaga tttggttaaa
     9361 gagatagaga aggatagaat tttctatgaa gaatcaggtg gtggagtaac tctatcaggt
     9421 ggagaagtaa tggctcaaga tatggacttc atttgtggag taataaacat gtgcaaatca
     9481 aagggaatcc atgtagcaat tgatacatgt ggctatgcaa agtcagaaaa ttatgaaaga
     9541 gttgctaaat gtgcagattt gtttttatat gatattaagc ttattgatga agataagcac
     9601 ataaaattta caggaaaatc aaatgactta atactgaaaa atgttaagat tttaagtgaa
     9661 ttaggggcta acataaatat aagaatacca ttaattgtag gagtaaatgt agatgatgaa
     9721 aatttagagg ttaaaaaaat gatagagttt ttaaagcctt tgaatataca agcagtaagt
     9781 cttcttccat atcataatat tggtaaacac aagtatgata aaatttataa aaagtatgaa
     9841 ggagaagaac tacaaagacc atcagaagaa aaattagagg aaataaaaag actgtttgaa
     9901 gcaagtaatt tcaatactaa gattggaggg taataaaatg gcaagaggaa cttttgagag
     9961 aactaaaaaa ttaagagaag aaagtattaa tgcagaacca cacatatcta ttgaaagagc
    10021 agtacttatg acagaagctt ataaaaaata tgagggaagt gtagagattc ctgtacttcg
    10081 tgctttatca tttaagcatt atattgagaa tagaactctt agcataaatg atggagaatt
    10141 aatagtaggt gaaaaaggtg attctcctaa tggagcacca acatatccag aaatttgttg
    10201 tcatacaatg gaagatttag aagtaatgca caatagagat ataataaatt ttagcgtatc
    10261 agaagaagct agaaaaatac ataaagaaga aataattcct ttttggaaaa agagacaaac
    10321 aagagataag ataataaatg caatgacacc tgagtggtta gcagcatatg aagcaggtat
    10381 gtttacagag tttatggaac aaagagcccc aggacataca gtttgtggag atacaatata
    10441 taaaaaagga tttttagatt taaagaaaga catagaagca agattaaaag agctagattt
    10501 tcttaatgat ttagatgcat ataataaaaa agcagattta gaagctatgg caatagcttg
    10561 tgatgcaatg gttatattag gtaaaagata tgctgagaaa gcaagacaaa tggcagaaga
    10621 agaaacagat gaagctaaga aaaaagattt attactaatt gctgaaactt gtgatgttgt
    10681 accagctcac aaaccagaaa cttatcatca agctatacaa atgtattggt ttgttcatat
    10741 aggggttact actgaactta atatatggga tgcttttact ccaggaagac ttgaccaaca
    10801 tctaaatcct ttctatgaaa gagatgtaga aaatggaata ctagatagag atagagcaca
    10861 agaattactt gaatgtttat gggttaaatt taacaatcag ccagcaccac caaaagttgg
    10921 tataacatta aaagaaagta gcacatatac agattttgca aatataaaca ctggtggaat
    10981 aaatccagat ggtcaagatg gtgttaatga agttagttat ataatacttg atgttatgga
    11041 tgaaatgaaa ttgatacaac ctagttcaaa tgttcaaata agtaaaaaga ctcctcaaaa
    11101 attcttaaag agagcttgtg aaatatcaag aaaaggatgg ggtcaaccag cattttataa
    11161 tactgaagca atagttcaag agcttatgga agctggaaaa actatagaag atgcaagact
    11221 tggtggaacc agtggttgtg ttgaaactgg atgttttggt aaagaagcat atgttctaac
    11281 tggatatatg aacattccaa aaatacttga attaacatta aataatggat atgacccaat
    11341 tagtaaaaaa caaataggta ttgaaactgg agaccctaga aatttccaat cttatgagga
    11401 actatttgaa gcatttaaaa agcaattaca ttatatgatt gacataaaaa tagaaggaaa
    11461 tgctgtgata gaaaatatat gtgcaaaaca catgccttgt ccacttatgt ctacaatagt
    11521 tgatgattgt atagaaaaag gcaaggatta ccaaagaggt ggagcaagat acaacactag
    11581 atatatacaa ggtgttggta taggaactat aacagatagt ttaacagcaa taaaatataa
    11641 tgtatttgat aagaaaaaat tcgatatgga tacattgctt aaagcacttg atgctaattt
    11701 tgaaggatat gaagctatac ttaatttagt ttcaaataag actcctaaat atggaaatga
    11761 tgatgattat gctgatgaaa taatgcaaga gatattcaat gcatattaca atgaggtaac
    11821 tggtagacca actgtttgtg gtggagaata cagagtagat atgttaccaa caacttgtca
    11881 tatatacttt ggagaaatta tgggagcaag tccaaatggt agactttgtg caaaaccagt
    11941 ttcagaggga atttcacctg aaaaaggtgg agatactaat ggacctacag cagttataaa
    12001 atcttgtgct aagatggacc acataaaaac tggtggaaca ttattaaatc aaagatttgc
    12061 accatcagta gttcaaggag aaaaagggtt agataatatg gctaatctag taagagcata
    12121 ctttaatatg gatggtcatc atatacaatt taatgtattt gataaaaatg tactgttaga
    12181 ggctcaaaag aatcctcagg attataaaga cttaatagtt agagttgcag gatatagtga
    12241 ccatttcaat aatttaagta gaacactaca agatgagata ataggaagaa ctgagcaaac
    12301 attctaggtt tgcttgtaaa taaaacttct tactatataa aatcgtaact gatataataa
    12361 agtataaatg gtttgattcc atttttaaaa ttttagatta attatgagct aactttctaa
    12421 ataggaagtt agctcgttaa gtttaatgaa aaaatcgtta aatttatatt tagtaaatca
    12481 tacacatatt agtaagtaat aca
//
LOCUS       DAEOSP010000120        12372 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.121, whole genome shotgun sequence.
ACCESSION   DAEOSP010000120 DAEOSP010000000
VERSION     DAEOSP010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.121"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            159..1550
                     /gene="hydA"
                     /locus_tag="KRH54_002463"
     CDS             159..1550
                     /gene="hydA"
                     /locus_tag="KRH54_002463"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBG8580115.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNPAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            1544..4105
                     /gene="xdh"
                     /locus_tag="KRH54_002464"
     CDS             1544..4105
                     /gene="xdh"
                     /locus_tag="KRH54_002464"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBG8580116.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRSKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            4257..5603
                     /gene="rpoN"
                     /locus_tag="KRH54_002465"
     CDS             4257..5603
                     /gene="rpoN"
                     /locus_tag="KRH54_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBG8580117.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            6401..7423
                     /locus_tag="KRH54_002466"
     CDS             6401..7423
                     /locus_tag="KRH54_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG8580118.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            7483..8490
                     /gene="gap"
                     /locus_tag="KRH54_002467"
     CDS             7483..8490
                     /gene="gap"
                     /locus_tag="KRH54_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBG8580119.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            8712..9914
                     /locus_tag="KRH54_002468"
     CDS             8712..9914
                     /locus_tag="KRH54_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBG8580120.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            9940..10683
                     /locus_tag="KRH54_002469"
     CDS             9940..10683
                     /locus_tag="KRH54_002469"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBG8580121.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            10692..12224
                     /locus_tag="KRH54_002470"
     CDS             10692..12224
                     /locus_tag="KRH54_002470"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBG8580122.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPAGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         5021 a         1406 c         2486 g         3459 t
ORIGIN      
        1 gatttaacta aatattagtt tagctgaata ttaatttaat taggtattgg tttaatctta
       61 atatagattt actacgtata agtgaactaa ctagattact ttaagttaat tttttcatat
      121 aaaataaatt gatacattac aagtaaggtg gagtctatat gggaattgtt ttaaaaggtg
      181 ggaaagtagt aacaagtgaa aaaacttact gtgcagatgt aagaatagaa gatgaaaaaa
      241 tagtcgatat aggtcttgat atatcaaaag aaaatgatga aatagttgat gttagtgatt
      301 cttatgttat accaggaggt atagatacac acacacactt tgacttagat acaggcgtta
      361 ctaagacagc agataacttt gagacaggaa caaaagctgc tatagtagga ggaactacta
      421 ctatattgga ttttgctaca caagctaaag gatgttcttt gaaagacgga ttagaagaat
      481 ggcatgaaaa agctaaggat aaatcctatt cagattatgg ctttcatatg gctataacag
      541 attggaatga ctcggtttgt aatgaaatgg aggatatggt gaaagaggga gtatcttcat
      601 ttaaactgta tatggcgtat aaaggaagcc ttcaagttga tgatggtgta atctttgaag
      661 cattgagaaa agcagaagag ataggaggaa ttattggttt ccattgtgaa aatggggaca
      721 taatatgtga acttgtggat aaagcaaagt cagaaaataa tttatctcca aaatatcatc
      781 aattaactag accagctata atggaggcag aagctacatc aagacttatg aagatagctg
      841 aagttgcaaa ttctaaagta tatgtagttc acttaagctg taaagaatcc ttagaggagg
      901 ttttaaaagc taagcaaaga ggtgtggatg taatagttga aacttgtcct caataccttc
      961 tattaaatga tgaattatat gatttagatg gatttgaaag tgctaagtat gtaatgtcac
     1021 cacctttaag aaataaagaa aataatgaaa tattatggaa tgctatttta gataatcaaa
     1081 tagacactat aggaacagac cattgttctt ttaattataa gggacagaag gaacttggga
     1141 taaacgattt cagcaagata ccaaatggtt ctccaggtgt agaacatagg atgagtcttt
     1201 tatacacata tggagtctgt aaaaataaaa tatctatcaa caagatggta gaagtaacat
     1261 caactaatcc agcaaagttg tttggaatgt atcctaataa aggaactata gaaataggca
     1321 gtgatgcaga tatattagtc ttaaatccaa acaaaaaatc caaaatagaa gcaaaaaaac
     1381 aattacaaaa tgtagattat acaccttatg aagggtatga agtggattgt caaatagaac
     1441 atgtttattt aagaggaaaa gaagtagtta gagatggaga attaatttgt aatactccaa
     1501 gtgggagata tataaatagg cgtctaacaa agggggctag agaatggtag aatttatatt
     1561 aaatggagag aaagttgttg caaaagaagg caaaaaactt ttagacttcc ttagagaaga
     1621 tatgaatata acttcagtta aaaatggatg ttcagaaggt gcttgtggaa catgtatggt
     1681 tataatagat ggtaaagcta ctaaagcatg tgtatttaaa actgataaat tagctgggaa
     1741 aaacataaca actatagagg gattatcaga gagagaaaga gatgtttttg catattcttt
     1801 tgctagtcaa ggagctgtac aatgtggatt ttgtattcca ggtatggtaa ttagttcaaa
     1861 agcacttata gacgttaata ataatcctac tgaagatgat ataaagaaag ctttaaaagg
     1921 caatatatgt agatgtacag ggtatgtaaa aataattaaa gctgtcgatt tagcggcaaa
     1981 gatttttaga gaaaatatag atgtgccaaa agtaaactgt aagggtttag taggtgaaaa
     2041 tcttcctaga gttgatgcag ttggcaaagc acttggatat tctgaatatg tagatgatat
     2101 gagaattgag ggtatgattt atggttcagc agttagatct aagtatccta gagctttagt
     2161 taaaaaaata gatatagaag aagcaaaagc ttatccagga gttgttgcag ttctaacagc
     2221 taaagatatt ccaggaacta taaaggtagg acacttaaag aaggattggg atactttaat
     2281 accagaaggt gaaataacta gatacttagg agatggaata gttttagtag cagcagaaac
     2341 tagagaagcg ttagaagagg ccaaaaagct agttaagata gattatgagg aattaacacc
     2401 attaagtact acagcagatg cacttagaga agatgctcca aagatacatg agacagggaa
     2461 tatacttgtt aaggaacatt tagtaagagg aaacgctaaa gaaaagatag aaaattcaaa
     2521 atatgttgta actaaaagat atactacacc tgctacagaa cacgcatttt tagagcctga
     2581 atgtgcagta gctggtcctt atgaagatga tggagtaata atctattcaa cagaccaagg
     2641 ggtgtttgcc actcaacatg aatgtgcaga tatgttagga ttaccatatg agaaggtaag
     2701 agttataaac aaaatggtag gaggagggtt tggtggaaaa gaagacatga gtgttcaaca
     2761 ccatgcagcc ttacttgcat accatacaaa gagatttgta aaagtacagt taacaagaaa
     2821 agaaagtatt attgttcatc caaaacgaca tgccacagaa atggaattta cgacagcatg
     2881 tgatgaaaat ggatacttaa cgggtatgaa ggcaacgata atatctgaca caggtgcata
     2941 tgcctctctt ggaggaccag tgctacaaag actgtgtact catgcagcag gaccatacaa
     3001 ttatcaagat attgatataa caggaacagc agtatatact aataaccctc ctggaggagc
     3061 atttagagga tttggagtta cacagagttg ttttgcaact gaatgtaatc taaatttact
     3121 tgctgaaatg gttggaatat ctccttggga gataagatat aaaaatgcaa taagacctgg
     3181 gcaagtttta ccaaatggtc aatttgcaga tgaaggaaca gcaatggcag agacactaga
     3241 agctgtaaaa gaagagtatg aaaagcatcc acgtgcaggt atagcttgtg ctatgaaaaa
     3301 tagtggggtt ggagttggta tacctgatgt tggaagatgt agattggtga taaaagatgg
     3361 caaagttcac ataagaacaa gtgcttcttg tataggtcaa gggcttggta ctgttatgac
     3421 tcaagtagtt tgtgaaactg ctaacctact tcctgaaaat atagttcatg aagttccaga
     3481 tactcattta acaccagact ctggtaatac tacggcttca agacagacag tatttacggg
     3541 agaagctact aaggttgcat caaataagtt aaaagaagct ttagactctg gcaaaacttt
     3601 acttgattta gagggagaag agttttatgg agaatatgaa agtattacag ataaaatggg
     3661 ttgtgacaaa gaaaatccag taagtcatgt tgcttatgga tatgcaacac aagttgtaat
     3721 tctggatgaa aatggaaaac tagaaaaagt agtagcagca catgatgttg gaagagctat
     3781 aaatccaatc agtttagaag gtcaaataga aggtggagta gttatgggtc ttggatatgc
     3841 tctaaccgaa gattacccag tagtcaactc tgttcctaca gctaagtttg gtacattagg
     3901 tttatttaaa tctacagata taccagaaat acaagcttta atagttgaaa agaacaaaga
     3961 tttattggct tatggggcaa aaggtgtagg agaaatttgt acagttccta ctgcaccagc
     4021 agtacaaaat gcctattatg tatttgataa agaatttaga actgagttac cactacaaaa
     4081 tactccatat agtaaaaaaa agtaacaaaa aaaagaaata ttaaataaaa aaatagatga
     4141 aattgtaaat aaaaaaaacc cttttgcata aggtatattt taaatgatag aatatacata
     4201 tacaggaggg tttttctttt ataaaataag tttaataaaa ttgagaggtg agcagaatga
     4261 attttaataa tagtctagaa ttaactcagt ctcagaagct aataatgact acacaactta
     4321 agcaatcatt atcaatatta aatatgagta agttagagtt agaagaagag ataaaaagag
     4381 aagcagagaa taacccatta gttgaagtcg agaaaagtgg ggagataaat tgggaagagt
     4441 atataaaaga catggatagg tcaagacgtt tagatagaac agagataagc tataatcctg
     4501 ataatgaagt taatctagaa aatttagtta aatattcgtc aaatttatat gaagatttaa
     4561 aatttcagat aagcctgtat aaacttacag acaaagaatt ggaagtctgt gagtatataa
     4621 ttgatagttt agatgaggat ggatatttaa gaactgagga aaaagtaatt gttgatacct
     4681 taaagataga tgaagaactt tttgaaaaat gtttaattag tgttcagcag ttggaaccta
     4741 gtggagtagg ggcaagaagt ctttcagaat gtctgatgat acaaatggct agtttaggaa
     4801 tatataatga aattttagag gaaatcgtta caaaagactt aaatttaatt gcaaataata
     4861 aatataaaga gataagtaaa aagtacaata tgtcattaca aaaatgtgtt gaactaataa
     4921 acataattaa gacattagac cccaaaccag gaagaacttg ttcagtagag aaaagtgtat
     4981 acattcaacc tgatgtaaca gtagaaaaaa ttgatgatga atttattgta tatttaaatg
     5041 agaaagattc ttatcaaata agaatcaata gttattataa agacatatta aaaaattcac
     5101 agtctgatga aagtgcaaaa gaatttataa aagaaagact aaattcagca acaggactta
     5161 tgaaaaatat agagagtagg aaaactacag tacttaagat tgcagaagag atagtaaaag
     5221 ctcaagatga atttttgaga aaagggacaa aatatataaa acctttgaaa atgaaagaca
     5281 tagcagaaaa attagaattt catgaatcta ctataagtag aggtgtaaat ggtaaatata
     5341 tgctaacacc ttttggcgta tatgaattta ggtacttttt cagtagcgcc attgagactg
     5401 agaataatga aatggcatct agtacaagca taaaaaaaat tataaaggaa acaataaaag
     5461 acgaaaataa aaagaaacct ttaagtgatg accatatatc taagttacta aaagaaaaag
     5521 gaatcaatgt tgcaagaagg acagttgcca aatatcgtga agaattagga atcccatctt
     5581 caagtaagag aaaagaatac taataagtaa aagacaaata tcagtatggt aaggggaaaa
     5641 ggctaattaa gagatgaatg tagattgggc taaaaatttt ataaagtaaa ataatataat
     5701 atgaaaaagt attaaaatgc tataaggtat taaaaaactg gggaacatta aaacattaag
     5761 atagcaaaga acataaaaaa ctaaagaaga ttaaaacatt aaagaacatt agagctttga
     5821 ggaatattag aactttaaag aatattaaaa tttataaaat ataaaaatac taaaaagaat
     5881 tgaaaaactg gggaacatta aaatattgta aaatgctaaa aaactagaaa acataattaa
     5941 ataagtgctt ataaagaatg attaaattaa ataagtgttt gtaaagaata attagatatt
     6001 agaaaaagaa gaatacctag ataaataaac tattattaaa tttaataatc acaaaaggaa
     6061 aacacgtagg gtataaataa aaatcttaac tagataaaag taaacttagt tgtagataaa
     6121 aataactaga aataaaagta actagaaata aaattattta tagtaataaa tattagaatg
     6181 aaaatataaa aaaatatata taaaaagcct tatataaaaa gaaaaaatac tgtgatttga
     6241 aaatggtgga gaaatatttt ataaaataaa aaaataaaaa agtttttgaa aaactattga
     6301 aatctatacg caaatcctat aaaataaaaa tatcttgtgg gacttaaata aacactaggg
     6361 acaaaaaaag tcccgaaagg aaagcaataa ggagtatgtt atgaagaact tattagaaat
     6421 tcaaaagaaa ttgattccac aggcaattga acttatggaa agaagatact ctattttaag
     6481 acaaatatct ttaagtgaac caataggtag gagaacatta tcaaatgtat tagagattag
     6541 tgaaagaata atcaggtcag aaactgagtt tttaaaagaa caaggactta tagacgtagc
     6601 tgtatctggg atgactatca ctaaagaagg ttctgatttg ttagataaac ttaaggacat
     6661 catgagtgac atcatgggat tgtctaagtt acaagataga gtcagagaaa agcttggaat
     6721 aaaaagggta atattagttc ctggaagctg tgatgaaaat ggaagtttac taaaagatgt
     6781 aactagatat ggatgcgaat atttcttagg cattttaaaa gatggagata tcgtttcaat
     6841 aacaggagga agcactatgt tggagttttc aaacgttatt aaaactgata agagatatcc
     6901 aaactctaca atagttccag ctagaggaag tatgggaact gatgtagaga tgcaatctaa
     6961 caatatagtt gcttcaataa gtaagaaatt acattctaat tgcaaacttt tacatatacc
     7021 agatgagttg gatgaaagtg caatgaaaac tcttagccaa gtgccagaga ttaagacaac
     7081 tttggagtat attaaaagaa cagacatttt agtttttagt ataggtagag cagatgtcat
     7141 ggcaaagaga agaaaacttc cagaagacaa ggttgatgag atattatcta aaaatgcagt
     7201 tggagaagca tttggatatt atttcaacaa agatggagaa atagtttaca aactgaacac
     7261 tgttggaatt gatttagaaa cagttaaaca agtaaaagaa actattgcaa tctttgcagg
     7321 gactgaaaaa gtagaagcct tattagctat atcaaatatg cataaaaaca tggttcttgt
     7381 aacagatgaa gaaagtgctt ataagatatt gtcattatta taaaagtcaa gacctagcac
     7441 agagctagaa aacatacatt tactttatag gaggtagcga aaatggttaa agtagctata
     7501 aatggatttg gaagaatagg aagattagca ttaagaaaaa tgatggagca acaagataag
     7561 ttcgaagttg ttgcaataaa cgacttaaca gacgctaaaa tgttagctca tttatttaaa
     7621 tatgatacag ctcaaggaag atttaacgga gaaatagaag ttaaagaagg tgcttttgta
     7681 gtaaacggaa aagaaataaa agtaacagca gaaagaaatc ctgctgacct accatgggct
     7741 gaattaggag tagatatagt attagaatgt acaggattct ttacatctaa agataaagct
     7801 gaagctcata tacaagcagg ggctaagaaa gttgttatat ctgcaccagc tactggagat
     7861 ttaaaaacaa tagtattcaa cacaaacagt gacatattag atggttctga aacagttata
     7921 tcaggagctt cttgtactac taactgttta gctccaatgg ctaaagtatt aaatgataaa
     7981 tacggtatag aaaaaggatt aatgactact atacatgcat acactaatga ccaaaatact
     8041 ttagatggtc ctcatccaaa aggtgattta agaagagcta gagctgctgc tggaaacata
     8101 gttccaaaca ctacaggagc tgcaaaagca ataggcttag taataccttc attaaaagga
     8161 aaattagatg gggcagcaca aagagtacca gttgtaactg gttcaataac tgaattagtt
     8221 tgtacattag gaaaaaatgt tacagttgaa gaaataaatg cagctatgaa agaagcttca
     8281 aacgaatctt tcggatatac tgaagaaatg ttagtatctt ctgacataat cggaataagc
     8341 tacggttcat tatttgacgc tactcaaaca aaagttatgg aagtagatgg aaaacaatta
     8401 gttaaagttg tttcttggta tgataatgaa atgtcttata cttctcaatt aataagaact
     8461 ttaggatact ttgcacaatt agcaaaatag tcagttttga gaaaatagaa aagtagattg
     8521 acaaagtccg agtttgtagt ttaatactac ggactcagga ctttttttaa gaaaaaatat
     8581 ataaaataca cctaaataat gttacatact atatataaaa atatgataat atgatatatt
     8641 taatagggta aaagtatgta ctatattata cttggcaata aaaaattact ttattaaaag
     8701 gagagatatt tatgtcaatg cttaacaaaa aaactataga agatattgat gtgtgcggaa
     8761 aaaaagtatt agtaagatgt gactttaatg tgccattaca agatggtgta attactgatg
     8821 aaaatagatt gaacggagct ttaccaacta ttcaatattt aatttctaaa ggggcaaaag
     8881 ttatattatg ctctcactta ggaaagccaa aaggagaagc taaaccagaa ttatctttag
     8941 caccagtagc aaagagatta tctgagatgt taggaaaaga agttgtattt gcagcagacg
     9001 ataatgttgt aggagaaaat gcaaagaaag caactgaaaa aatggaaaac ggagatgtag
     9061 tattattaga aaatactaga tatagaaaag aagaaactaa aaacgaagag aatttctcta
     9121 aagaactagc atcacttgct gaaatatttg taaatgatgc atttggaact gctcatagag
     9181 cacattgctc aacagttggt gcaggcgagt ttttacaaga gagagtttgt ggatacttaa
     9241 ttcaaaaaga attaaaattc ttaggagaag ctgttgcaaa ccctgtaaga ccatttacag
     9301 ctatacttgg aggagctaaa gtttctgata aattagctgt tataaacgaa ttattagaaa
     9361 aagttgataa tctaataata ggtggaggaa tggcttatac attcttaaaa gctcagggtt
     9421 atgaagtagg aacttcttta cttgaaatag ataaagttga atatgctaaa gaaatgatgg
     9481 aaaaagctaa gaataagggt gttaacttat tattaccagt agacgttgtt atggcagacc
     9541 actttgcacc agatgctact ccaatagtaa cagaagatgc taatgtaaaa gaagattaca
     9601 tgggactaga tatgggacct aagactatag caaactttgt aaaaactata aaagaatcaa
     9661 aaactgtagt atggaatggg ccaatgggag tatttgaatt tgaaaacttt gcaaatggta
     9721 cattatcagt agctagagct atggcagagt tgactgatgc aactactgta attggcggtg
     9781 gagatagtgc agcagcagtt aatcaattag gatttggtga caagatgact cacgtatcaa
     9841 caggtggagg agcatcacta gaatttttag aaggtaaaga attaccaggt attgcagcat
     9901 tagataataa ataaactaat atatagagga gattgtaaaa tgagaaaacc tataattgca
     9961 ggaaactgga aaatgcataa aacaataaaa gaagctttag aatttgtaaa tgaagttaaa
    10021 gataaagtaa attctgataa agtagaagct gttatatgtg caccatttac tttattaaag
    10081 gatttaaaag aagctactaa gggtacaaat ataaagatag gtgctcaaaa tatgcacttt
    10141 gaagaaaaag gagcatttac aggagaagtt tcacctctta tgttaaaaga aatagatatg
    10201 gactatgttg taataggaca ttcagaaaga agacaatact tcaatgaaac tgatgaaact
    10261 gtaaacaaga aagttttaaa agcattagaa gtaggaatag acccaatatt atgtgttgga
    10321 gaaactttag aacaaagaga agctggaaaa actaaagatg tttgtaaagt tcaagttgaa
    10381 aaagctttag aaaatgttct taaagatgat ttagcaaaag tagttgttgc atatgagcca
    10441 atctgggcta ttggaactgg taaaactgca actgctgaag atgctaatga cgttatatct
    10501 tatataagag aagttataaa aggattatat ggagaattag ctaatgaagt tagaatacaa
    10561 tacggtggaa gtgttaaacc ttcaaatgta gccgaaataa tgggtcaaag tgatatagat
    10621 ggagctttag taggtggagc tagtttagct agtaatgatt acctagacct tgttaatttc
    10681 taaggagtga aatgatgaaa aaaccagttg ctttaattat tatggacggg tttggataca
    10741 ataaggatgt taagggaaat gcaattgctg agtccaaaac tcctaattta gatagaataa
    10801 aaaaagagta tccaaacaca ttgataaatg ctagtggtct tgatgttggt ctacctgatg
    10861 gtcaaatggg aaattcagaa gttggacaca ctaacatagg tgcaggtaga atagtatatc
    10921 aagatttaac tagaataaca aaatctataa aagatggtga cttttttact aataaagttt
    10981 tatgtgaagc aatggataat gcaaaagaaa attctcttca tgtaatggga cttttgtctg
    11041 atggaggagt acattcacat atagaccatc ttaaggctat aatcaaaatg gcaaaagata
    11101 aaggcgtaca aaaggtgtat gttcacgcat ttactgatgg tagagatact gaccctcaaa
    11161 gtgcattaga atatgccaaa gaagtacaag ctagtatgga tgaaattgga gtaggtgaat
    11221 ttgctacagt ttctggtaga tactacgcta tggatagaga taagagatgg gaaagagtag
    11281 aacttgcata caatgccatg gttagaggta ttggtgaaaa agcaaattct attgaggaag
    11341 ctattcaaaa ctcttatgat gatggaaaaa atgatgagtt tataatgcca acagttatta
    11401 tgaaagatga taaaccagct ggaagtataa aggaaaatga ttcaattata ttcttcaact
    11461 ttagaccaga tagagctaga caaataacta gagctttagt ttgtgaagag tttgatggat
    11521 ttaaaagaga agatattaaa aacttctttg tatgcttaac agagtacgat ataactatag
    11581 agaatgtgca tatagcattt ggacctcaat ctttagccaa cactctaggt gagtatctag
    11641 ctaaaaatgg aaaaactcaa cttagagctg ctgaaactga aaagtatgca catgtaactt
    11701 tcttcttcaa tggtggagta gaagagccaa ataaaggtga agaaagacta ttgatacctt
    11761 ctccaaaggt tgcaacttat gatttaaaac cagaaatgtc agcatatgaa ttgacagata
    11821 aagctttaga taaattagga gaagataagt ttgactttat agtattaaac tttgcaaatc
    11881 ctgatatggt aggtcataca ggaagtatag aagcggctat aaaggctgtt gaaactgtag
    11941 atacttgtgt aggaaaattg atagataaaa tagtagagtt gggtggaagt gcaataataa
    12001 ctgctgacca cggaaatgct gaatatatgt tagacccaga aacaggaaaa actgtaacgg
    12061 ctcactctat taatccagta ccatttattg tagttggtca agaatatgaa agtgcaaaat
    12121 tattggatgg aggaagatta tctgatatag caccgactgt tttagatatg atgaagttag
    12181 aaaagccaga agaaatgact ggtcattcac ttatttcaaa atagtcgatt ttgataagta
    12241 gagaataata tataaactac aatataaatt taaaaataat gcagtttagg tttattttgt
    12301 aaagtttaga ctttatgaca taagatttaa attttgtaag ttctggatat tgtaaggtgt
    12361 agatgaagat at
//
LOCUS       DAEOSP010000121        12357 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.122, whole genome shotgun sequence.
ACCESSION   DAEOSP010000121 DAEOSP010000000
VERSION     DAEOSP010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12357
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.122"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            13..1713
                     /locus_tag="KRH54_002471"
     CDS             13..1713
                     /locus_tag="KRH54_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580123.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            1883..2500
                     /locus_tag="KRH54_002472"
     CDS             1883..2500
                     /locus_tag="KRH54_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580124.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            2861..4054
                     /locus_tag="KRH54_002473"
     CDS             2861..4054
                     /locus_tag="KRH54_002473"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBG8580125.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            4072..5364
                     /gene="lysA"
                     /locus_tag="KRH54_002474"
     CDS             4072..5364
                     /gene="lysA"
                     /locus_tag="KRH54_002474"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBG8580126.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYVEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            5581..6240
                     /locus_tag="KRH54_002475"
     CDS             5581..6240
                     /locus_tag="KRH54_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG8580127.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(6395..7480)
                     /locus_tag="KRH54_002476"
     CDS             complement(6395..7480)
                     /locus_tag="KRH54_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBG8580128.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            7671..8387
                     /locus_tag="KRH54_002477"
     CDS             7671..8387
                     /locus_tag="KRH54_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBG8580129.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            8445..9023
                     /locus_tag="KRH54_002478"
     CDS             8445..9023
                     /locus_tag="KRH54_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG8580130.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            9417..10289
                     /locus_tag="KRH54_002479"
     CDS             9417..10289
                     /locus_tag="KRH54_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBG8580131.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     repeat_region   11120..12329
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=11120..11148
                     /rpt_unit_seq="gttttatattaactatatggaatgtaaat"
BASE COUNT         5000 a         1274 c         2125 g         3958 t
ORIGIN      
        1 gaggagtatt aaatgaaacg atatcttaaa acatatggag ttgcattaat aattgtaata
       61 atttatgcat ttattaaatt acctgtacta agattagatt ttacatcttt attttcagta
      121 ggaataattt tctttgttgt agcaggaata ctagatatga tgttagatag aaatgaaaga
      181 gcatctaaaa tggctaaata taatttttca atagcaatag ctttgcttgt gtatattgta
      241 gtagttccct ttataacttc tacacctgta cttcatgcaa aatcatatag agaactattg
      301 ggaaaagtaa ctgaaagtaa atttactgat gacattagcc ctgtcagtgt aaatgacatt
      361 cgtcttgtag atgaagatat ggcaatgaaa ttaggtgaca aaaagcttgg agaagttcct
      421 gcaataggga gtgtatccaa attaggaaaa tttcatatac aaaatgtaga tggagagttg
      481 tactgggtag cgcctcttgt tcacagagac ataattaaat ggataacatc tctaagtggt
      541 acaagtggtt atgtaaaagt atcagctagt aatcctcaag acgtccaatt agttcaagaa
      601 atagatggaa agccaataaa gatagtttac caaccagaag cctacttaca tcaggattta
      661 caaagacatt tatacataca tggtattgta aatgttggta tgacagattt cactcttgaa
      721 ataaatgatg aaggaagacc ttattgggtt gtaagtttat atgaacataa aattggctat
      781 ggaggagcaa atgcaacagg aatagccact gttgatgcag aaactggaaa aataaatgtt
      841 tatgatgtta aaaacacgcc taaatgggtg gatagaatac aaccacaaag ctttgtaaca
      901 gaccagatta aagactgggg tgtttatgtt aatggatttt taaactctgt tatatcagaa
      961 aagggagttt tagtaccaac agaaggtaca tcattagttt atggcaatga taacaggtct
     1021 tattggtata caggtataac atcttcaggt ggagatgaat ctacaattgg atttatgtta
     1081 gttgattcta gaacaaaaga agctagatta tataaacaac caggagctac tgaaacggct
     1141 gcaatgaaat cagctgaagg taatgttcaa gaaaaaaatt atgaagcgac gttccctgta
     1201 atgtataata tattgggtca gccaacatat gtatcatcat taaaagataa ggctggtctt
     1261 gttaagagag ttgcatttgt atctgttgaa gattataatg tacttggaat aggtgaagat
     1321 aagaatgaag cattaagaaa ctataaggat gcattagaat ctaagggtaa tgatttaaaa
     1381 cttgataatg atttaaaaga tgaggtatta gaaggcacag tgacaagaat aagcccagat
     1441 gtgcgaggtg gtaacactaa ttattatgta actttagatt caaataaaga tataatattt
     1501 agggctactt caaaagtatc tagcgaattg ccattgactc aggttggtga taaagtcaaa
     1561 atatcgtatt caaaagaaga atctggtgta atagaaatgt cagaatttga taacctaaat
     1621 attgcaaact ttgaagataa aaaagcaaaa gaaaaaacag gaactgatga aaatacagat
     1681 tccaaagatt ctgaaaatca aggtaatgta taatttaaat aaaaagattg ataatagtat
     1741 ttaacatagt gatagctaat ttttatagaa tttaattcta taaaaattag ctattttatt
     1801 ttaattcttt gtaaatttaa tgtcataaaa ttaatataat attagtacaa ctttgcaaat
     1861 aatacttcaa ggagaatgta ctatgacgaa tatggaaaaa gagatattac ttttaattag
     1921 cagggttaga tgtgttacag agtctcaatt tagaaaaatt tatggagacc aaaaaagata
     1981 caataaaaaa aattttaaaa aaactttgag aaaaatgtgt agtgaatata cactaaaaaa
     2041 atatccttgt gatgtggatt attataatta taaagacctc tcttatgtat actatttaaa
     2101 cggtagtaaa ctttttaagg gagatgattt aataaaggct ctagtaggat cagaattagt
     2161 tgttagaatc aatactgcag gttgtgatgt aaaaagattt taccgaaatg ttagtgtaga
     2221 tgatgtgaaa tacgatttat ttattgaata tacagaccaa tttggtaata taaagcaagt
     2281 tttagttgat gttgacttaa ataatgaaat agacatttct aaatacgaaa atctttctaa
     2341 aaaaatagat aaatctacaa ttccattttt taaagtacca aacgttttgg tagttacacc
     2401 agataatcta tccgaattaa atgatttaga gagatatgat aatgagtata gcataaattt
     2461 tatagattta ggattaaata aactatttaa ttgtatataa gctaacttta gaaaagaaaa
     2521 ctatcgattt gccacatttt gtaaaataat tgttgcaata ttatatagaa tatgataaaa
     2581 ctatattaat aaattaatta aaaataatga gcctagggta gaggtagctg tattcaatag
     2641 tattcaatag gagctggtaa gcgttgataa ttgatgaaag ggtatatagc cgaagaaatt
     2701 ttgttgacgg acaaattttc tggacttatg cagaatatgc ataaggctgt cacttttaaa
     2761 gtggtgagct acaaggttac ttggtctatg aaatttacac gtccaaagta tacctttggg
     2821 cgtttttatt tatcaaaatt tagcttgagg gggagaaagc atgagtataa ttgttcaaaa
     2881 atatggtgga agttcagtag cagatactga aaaaataaaa tcaatagcag aaaatataat
     2941 agaacgaaga aaagaaaatc cacaaatggt aatagttgtg tctgccatgg gaaaatccac
     3001 tgatgaatat ataactttag caaaggaatt gagtaatgaa cctagtaaga gggaattaga
     3061 tgctttaatg tctactggtg aaatgatttc agcatcttta ttatcaatag cattaaatgc
     3121 acttggatgt aaagctataa gttataatgc ttaccagtta aatataaaaa cgagtggtct
     3181 tcatggaaag tctcaaattg atgatataaa cgtaaacaga attaaaaata gtttagatga
     3241 aggaaatgtc gtgatagtaa ctggatttca gggaatcaac gaagatggtg atgtaaccac
     3301 tcttggaaga ggtggttcag atacatctgc tgtagctctt gcggttaaat taaatggaaa
     3361 atgtgagata tatacagatg ttgatggaat ttattttaca gacccaagaa aatattcaaa
     3421 ggctagtaaa cttgatgaga ttgaatatga agaaatgctt gagttagcga gtttaggagc
     3481 acaagtaatg cattcaagaa gtatagagct tgcacaaaaa tatggtgttg aaatctatgt
     3541 cggacgtaca tgtggtacag aaaaaggaac atatataaga gggggaaaag acatgaaatt
     3601 agaggataaa gtaataacag gattagctac tagtgatgat gacagttcta taacaataaa
     3661 agattttaaa gctgaaaata tatctagctt atttgaagat atagcaacta taggtataag
     3721 tgtagatatg ataagccaaa ctgcacctat tttagataag ataagtgtat catttacagt
     3781 tccaaaagaa gagttgggag aatgtaaaaa gatagtatct aaatatacag atgaagaaca
     3841 tgtagtgatt gataataata tcactaaatt ttctttagta ggtcttggta tgaaaaatac
     3901 ttctggagtt gctgctaaag tatttaagat attcaatgaa aatggtataa tgataaaact
     3961 tataactaca tcagagattc gtataacttg tgctataaat tctgatgata agcaagtagc
     4021 tatagaaaaa atagcagaag tatttaatat ataaaatttg gggaggctat tatgaaactt
     4081 catggaacta tgactgtaaa taatgatgaa ttatatattg gaggagtgag ttgtttagaa
     4141 cttagtaaaa aatatcaaac tccactttat gtatttgatg aagctttagt tagacaaaat
     4201 tgtagagaat atgtagaata ttttaaggct agagagggaa aaaataaggt tgcttatgca
     4261 gggaaagctt ttttacctct ttacatgtgt aatcttataa atgaagaaca aatgtgttta
     4321 gatgtagttt caggaggcga gttatataca gcttataaat caaattttcc tatggaaaaa
     4381 gttttctttc atggaaacaa taagactaaa gaagaaataa acatgggatt agatttaggt
     4441 gtaggtaaat ttgtggtgga taatttttat gaattagatt tgcttgaagc attatgtgaa
     4501 gataaaggta aaactcaaga gatatacttt agaataactc ctggtataga agctcataca
     4561 catgattata taaaaactgg acagatagat tctaaatttg gatttgcttt agtaaatgga
     4621 gatttattta gagcaataga aaaattaagt gaatataaaa atattaaact tgtgggtctt
     4681 cacgctcata taggttcaca aatatttgat gtagaaccat acatagatac agtggatata
     4741 atgatgaact tggtaaaaga aataaaagaa aaattcaaca tacaactaac agaaattgac
     4801 ctaggtggag gagttggcgt ttattatagc gaaggagata aacctaaaac tataaaagag
     4861 ttttgtgaaa ctataatcac taaagcagaa acttcatgta aagagcttgg aatagaagta
     4921 cccacattgg taatagaacc aggaagatca atagttgcaa atgcaggaag tacattgtat
     4981 acagtaggtt ctataaaaga tataaaagat gttagggttt atgtaagtgt agatggaggt
     5041 atgacagata atgtaagacc ttcgttgtac caagctagtt atgagtgtag tattgtgaat
     5101 aaaattaatc atgaggctat gaatcatgta acaatagctg gaaaatgttg tgagagtgga
     5161 gatatattaa ttggagatat aaacataatg gatattaaaa gtggagatat acttataaca
     5221 acatcaactg gtgcatatgg atattcaatg tcttcaaatt ataataaaat accaagagca
     5281 gcagtagtat cagtatttga tggaaatgat aagataattt gtaagagaca aagctatgag
     5341 gacttattag ctttagagat ataaaatgga gaagtatata ataaaaatta aatattaaag
     5401 tttattatga gcagatttgc taggatatgt atctctaaat taatttttgg tttattgtat
     5461 cgttcgtagc atatctgctt attttttgtt ttactaacaa atttcttgta ataaatataa
     5521 aatattttaa aaactttata attatgataa aatttaaagt atatgacaag gagggattta
     5581 atgagaggaa gagtaatttt attaatatcg atattatcta tatttcttgt aggatgttca
     5641 tttaataaaa aagatgaaat aaatttagta gagcaaggaa aaacatattt agaaaaacat
     5701 aactataaaa aagctatgga atcattatcc tctgcactgg aagaagatag tactaatgag
     5761 aatgcaagag ctatgtatat gcaagctatg agaatgtcaa atatgacgga gtttgaagaa
     5821 ttaaaaaatt atgagggtgc aatcaaggaa cttaaactta tagagaaaat caaaaatggt
     5881 tcctctgtta ttaaagaaga ggcaactaaa aagaaagaag agttaactaa attagaaaaa
     5941 gaacaaaacg aagcagagga acaaagaaag aaaaaggcaa aagatacggc tggagaagat
     6001 aggtatagag ttgaatcaga tgcaaggaaa tcttcttata gtggaagtag taaaaataat
     6061 aaatctagtt ctaaaaataa tgataaaaaa tcagatagta ataagtctga caataataac
     6121 agttcttctc aaaatcaaga aaagccaaat aatagtaatc caacaccagc accaactcca
     6181 caagatacta ctggtggttc agatagtggt ggaaattctc aaaactcaaa taatcaataa
     6241 gtataaaata tatgcataaa tttaatgaac aaattatata ttataaaaaa tatctcttag
     6301 caattttttc ttactaagag atattttttg agaaataatt taataaataa cagttaaatt
     6361 ttaaactaaa aattaaagtc acagtagaga taagctattg taaacgatta tctatatatt
     6421 tatcaacata agtttttaca acagcagcta ttggtactga aagaaaaact cctatgaaac
     6481 caaatagaga ttgtcccacc aaaatagcag atattaccca tatagctcct ataccaactt
     6541 gattacccat aacttttggt tctataaaat ttccatctaa ttgttgcaat ataaatagaa
     6601 atgcaatgac ccatatagtt ttttgtggac tatataatat agtcatggct attggaggga
     6661 ttgcaccaat aaaaggacca aaatagggaa ttatattacc taagaatatt attgaagcta
     6721 aaaataaagc attttctatt ctgataacaa attgaaatcc taaaaatgca attataccaa
     6781 taataagaga gtctaatatt ctaccaagta aataatcata gaaaatatca tgagtagtcc
     6841 taaaaaattc tataatatta tctgccttgt ttctagcagt agaagaatat agaaatctct
     6901 taaatcccaa tgcaattttt tctttatcat atataatgta tatagatagt ataaatccca
     6961 ttgtcatatt aaatagcata gaagatatac taagtgtata gattaagaaa tttttaggaa
     7021 ttttaccaac aaaattactt gcttctttta ataaattatc taaactatgt tgtatatgag
     7081 gaattaaagt ttttagaaaa tctatgtttg acatattttt ttctataaaa ctatctatat
     7141 aatcagtata cataggcatt tcatttatca atctatttaa tgtattagct attgatggaa
     7201 ttaataacct aatggcaaag gctaaaataa aacctataaa tatataagat aaaaaaatgg
     7261 ctaaaagtct atgtgctttg aattttacct caaaaagcat cacaagtgga tttaaaagta
     7321 aagcaagtaa aatagctaat aaaaatgggc taaaaaactt taagaatcct tcaatactag
     7381 atataaagtc tgatggagta ttaataaatt taaaaaataa aattccaata aacacgacta
     7441 ttaaataata ttttatattt tcctttgaaa taataatcac ttccttaaaa tagtgtttag
     7501 tcagctatta catattaata gtatgtgaaa agaaattaca attatttagg aaaattaaat
     7561 aatttttttg cttgttttta attaaggtct agtatagtat aataatgtag caaaattaac
     7621 attgataaaa atattaaaca cacataattt aagataagga aggattaagt atgagaatta
     7681 ataagtatat agcttcttgt ggaatagcat caagaagaaa agcagaagaa ataattcttg
     7741 gaggaagaat aaaggtaaat ggaagtgtag taaaagagtt atcttttaat attgatgaag
     7801 aaaaagacat agtagaattt gataataaaa aagtaaaacc aagtgaaaat tatatatata
     7861 tagtattaaa taaaccagag ggatatataa ctacagtaaa agaccaattc aatagaccca
     7921 gtgtaataga catacttaaa gatgtaaggg aaagagtata tccaataggg agattagatt
     7981 atgaaactag tggattatta atacttacta atgatggcga tttaacatat aagcttaccc
     8041 atccaaaaca tgaaattgat aaaacgtatg tggcaagtgt gaagggaatt ataagtgatg
     8101 atgagataag acaattcgaa acaggactaa aaatagaaga ttacacaacg gcacctgcta
     8161 aaataagggt aactaaagaa aataaagaaa aaaattattc agtgtgtgaa ataactatac
     8221 atgagggtag aaatagacaa gtaagaaaaa tgtgcaaagc tataaatcat ccagttttaa
     8281 atttaagaag gatttctgtg gggaaaatag tattaaaaga caccaaagtt ggagaatata
     8341 gatacctaac agaagatgaa ataaaatatt taaaatcaat aaaataagat aaaaataaat
     8401 tgagaaaaaa tgaaataaag ttaatagtag ggagaaatag tattatggca tatgcaaagt
     8461 atttaactaa aattacagat ataaataaat tttatttaga aaatattata aatgaaggtg
     8521 acatagtaat tgatgctact atgggaaatg ggtatgatac aaggtatcta gctgagaagg
     8581 ttggagaaaa gggctttgta tatgcttttg atatacaaga agaagcaata aagtctacaa
     8641 gaaagaaact agagaaagaa ggatatacag atagagtaaa attgatattt gatgggcatg
     8701 aaaaaatgaa tcaatatata aaagaagaag tttcatgtgt tttatttaat ttgggttatc
     8761 ttccaagagc gaaacattca attataacca aaccagatac aactttaaag gctataaagt
     8821 taagtttaga aatgttaaaa gaaaatggag taataagcat tgccatatat acagggcatg
     8881 atggaggtca agaagaaaaa gattgcatat ataaccttgt caataattta aatcaagatg
     8941 aatttaatgt tttagaatct aagtttttaa atcaagtcaa taacccacca caattgattt
     9001 taattgaaaa gaaaaaagca taataaatac taaaaaactt gacaagattg tattaagagt
     9061 taatatatat agtgtgacct attaaaaata ctttaaataa taagtataca acagcttatt
     9121 ctcaaaacaa aatatacatc taaatatgca aaataagata gataactttt aaatgagtaa
     9181 aaattgaaaa atttaaaaga aatattggat aaatattaaa aaatgcaagg aaagatggta
     9241 taataaattt aatcgaaaat actatgtgaa atataaggta cagaaaagtt agaattttat
     9301 gagtttaaga agctatgata aaaactaaga tgaaattgag ataaaaaata aaatttttag
     9361 agaaaaagac agtgttaata aaacaaaata gtttatataa ctaaaaggag aaaaaaatgg
     9421 aagaaacaaa tgaaatgaag gacagtaaac atttgatatc aaatatacaa tctcaatata
     9481 cgagattgag taagggtcaa aaacttatag cacaatacat tttaaataat tatgataaag
     9541 ttgcatttat gactgcatgt aaattgggtg aaactgtagg agtaagtgag tctacagttg
     9601 taagatttgc aaatgcatta ggttatagtg gatatcctaa attacaagct gcacttcaag
     9661 aactcataaa aaataaacta actacagtac aaagagttga aatggcacat gattattccg
     9721 atgattttgc aatactaaac aaagttttaa aaagtgatat agataatata aggtctacac
     9781 tagaagaaat tgacgagaga gcgtttaaag aagcttctaa caaactttta agagcgagaa
     9841 aaatatatat acttggaatg aggagttctt ttgtagtagc tcaatattta ggtttttact
     9901 tagatataat acttgacaat gttcatataa ttagaatgga tatgggagat gcttttgagc
     9961 agattgtaag aataaatgaa gaagatgtaa tagttgctat tagtttccct cgttattcta
    10021 agaaatctta tcaaattgtc aattatgcta aggaaaaagg agcccatgta atatcattaa
    10081 cagatagctt atttgcgcca gtagcatcac ttgcagataa cactcttcta gttaagagta
    10141 atatggcttc atttgtagat tctctagttc cagctcttag tatttcaaat gcccttgcta
    10201 tatctgttgg gatgaaggaa aaagaagata ttaaacagca ttttgatgat ttagagcaaa
    10261 tttggaaaag atattctgtg tatgagtaaa gtttgtatat taataagtat tattaattat
    10321 taaaatgttc aaatatagtg atgtttggat aaaatatata aaatagctga ctggcattta
    10381 actgacagtc agttttttta tttaaacaat attttgctta aattttaagg aatatctttg
    10441 tctatttctt ctaaatgaga gtatttattc atagttattt tcatttatta tatttcttgt
    10501 tgattatata tttttttata gaaaagaaaa tagttatggt tttgtatctg ttatttttgc
    10561 acttattcaa tttatttttg tgaaacctgg aaagataaaa ctttaatagc agataataac
    10621 cttgagattg taaagtaaca aagtgaaaat gtataaagag gaaaagaaat ggaaggaaga
    10681 aaagtatata gataaagaga tataacacgt attttgattt aactgtataa gatgaaaaat
    10741 ttgatgattt tgaataattt aaaaaattgt taatgaaaat aaaagactta ttgagctaag
    10801 gtgcgattac ataggaagga tgcaataaaa agaaaaagga atcattggaa tagagaatat
    10861 aataaatact tataagaatg tagatgcttt tagtttgtaa aattatccca tttttatttt
    10921 atagtatgag ttttatgata taataaaaat ataaaagttt tgcagtgagc gatttttgtg
    10981 ataaagtagg gtttaatagt tgaaatataa agcgttgaga gtgtatgata actgttatca
    11041 attgcactat tgctcgctca ctgcaagttt aggagaattg tatatgtata agtattggaa
    11101 atacttaatt tattttgggg ttttatatta actatatgga atgtaaattt tctttttgaa
    11161 gttgttgacg ctctttttca tcagcgtttt atattaacta tatggaatgt aaatacatca
    11221 agactatcta tattattaag agtaacttta gttttatatt aactatatgg aatgtaaatc
    11281 taacttgttt ccctctaaag cgtgtttagt agggttttat attaactata tggaatgtaa
    11341 atctcttttg cgcagagtgg ttttttactt gtctaaatag ttttatatta actatatgga
    11401 atgtaaatta aagacctttt ttcatgttgc actctaaaaa tctaagtttt atattaacta
    11461 tatggaatgt aaatgataag gaatctgaat attaataaca ccagaatctc cgttttatat
    11521 taactatatg gaatgtaaat ctactagcag gaacatcaaa aggaggttta aaatccgttt
    11581 tatattaact atatggaatg taaataccaa aaggaatgtt tatttttgac tttgcttatc
    11641 aattgtttta tattaactat atggaatgta aatatacttg gtcaatacat ttcaaaagtt
    11701 gcaacagaac gttttatatt aactatatgg aatgtaaata acttatcagc atctttttta
    11761 taagttccaa ccacgtttta tattaactat atggaatgta aatttatcat tttttccgct
    11821 tctgcttgta atttagactc gttttatatt aactatatgg aatgtaaatt tccctaatgc
    11881 tccattttca ttataatctg gtgtagtttt atattaacta tatggaatgt aaatgtaggt
    11941 gtcggagagc taggaaacta tagcataatg gttttatatt aactatatgg aatgtaaatc
    12001 gttcttggaa tgctaaaact cgtccttctt ggaataagtt ttatattaac tatatggaat
    12061 gtaaatctcc caggagtacc gctataaaaa taaatcatac aatgttttat attaactata
    12121 tggaatgtaa ataatttttg aaatgcactg aatactcatt tattacatcg ttttatatta
    12181 actatatgga atgtaaatag gaacaaattg ctaaaaaatt aggggttggt tattcgtttt
    12241 atattaacta tatggaatgt aaatctgaga aagttttatt cttttttaat gttacaaatc
    12301 gttttatatt aactatatgg aatgtaaata aattattccc taaaaaaagt ttatttc
//
LOCUS       DAEOSP010000122        11827 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.123, whole genome shotgun sequence.
ACCESSION   DAEOSP010000122 DAEOSP010000000
VERSION     DAEOSP010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11827
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.123"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..659)
                     /locus_tag="KRH54_002480"
     CDS             complement(<1..659)
                     /locus_tag="KRH54_002480"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG8580132.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLNTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     I"
     gene            complement(1273..1811)
                     /locus_tag="KRH54_002481"
                     /pseudo
     CDS             complement(1273..1811)
                     /locus_tag="KRH54_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
     gene            complement(1888..3693)
                     /locus_tag="KRH54_002482"
     CDS             complement(1888..3693)
                     /locus_tag="KRH54_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8580133.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(4089..4466)
                     /locus_tag="KRH54_002483"
     CDS             complement(4089..4466)
                     /locus_tag="KRH54_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBG8580134.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            5371..5856
                     /locus_tag="KRH54_002484"
     CDS             5371..5856
                     /locus_tag="KRH54_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580135.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(6056..6595)
                     /locus_tag="KRH54_002485"
     CDS             complement(6056..6595)
                     /locus_tag="KRH54_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG8580136.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(6588..7001)
                     /locus_tag="KRH54_002486"
     CDS             complement(6588..7001)
                     /locus_tag="KRH54_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8580137.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(7037..8158)
                     /locus_tag="KRH54_002487"
     CDS             complement(7037..8158)
                     /locus_tag="KRH54_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG8580138.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILIPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            8757..10409
                     /locus_tag="KRH54_002488"
     CDS             8757..10409
                     /locus_tag="KRH54_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBG8580139.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAIPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            complement(10768..11694)
                     /locus_tag="KRH54_002489"
     CDS             complement(10768..11694)
                     /locus_tag="KRH54_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG8580140.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
BASE COUNT         4038 a         1927 c         1324 g         4538 t
ORIGIN      
        1 tctatagctt ctttagccat agttccatat ccttgcatta tccatgctgg tatttcttca
       61 tatccgtccc atgctgtatc ttgaaccatt actccatggt catcagcttc tgcataatca
      121 ttagctaatc ttactgcatc atcatagttt aagtctgtta tactagcatc tgcaccttct
      181 cctttgattt tatttaatct aaattcagaa gaaccttttg gcattaatac aacagatttt
      241 tgacctaatt tattagctgt ccaagcaact cctctaccat gatttccatc tgtggctgta
      301 aagaaagtta tttctcctaa tttttctttt atttcttttg aagttaattt atcataccct
      361 aactcagata tatctgtatt taatctttga gctaagtatc tacccataga gtaagaacca
      421 cctaaaactt taaatgcatt taaaccaaat ctataagatt cgtctttaac atatattccg
      481 cttacgccta gtgattttgc taagttatct aatttaacta aaggagtctt ttcatattgt
      541 ggaaagcttt catgaaaagc tttagccttt gcaatctctt catcttttaa aaattcaatt
      601 cctttttctt tttcctctga tttaggtaag ttatttaatt tccattttat ttctctcatt
      661 tattttatat tcccctttca aatttttcgg tgtcatttta attttttatc tttgtgcctt
      721 atagcaaagc aattaccgtg ccaatattat ttattctaaa aacaattttt ttatgttgga
      781 attacatact tttataaaat ttctttaaag tttataaatt ttgagtgtga aaaataagat
      841 ttataaaaga aaaaattctc atattgggaa atttttatca atacgagaat tatcaattcg
      901 agaaatcgtt tatttgtctt aattcttcct ttattatgct cttatttttt atatgtctat
      961 tgttttgtca gatatattac tacttttatt ttctataaat atatggctat aaccttctta
     1021 cgcataaact ctataaattt atatacttta tatactttat attctttatt acccaaagcc
     1081 tccaaaatgg cttaaatctt ttcatagctt cttacagaag tttacatggt catcccccca
     1141 ataagcaaaa gtgcaatatg tgtaccattg tgccctactt gcgactattg agctgtcgaa
     1201 attcgcaact tcgcatttat ctgatgaaat gcactataca aaatattcat aacatccaca
     1261 acagagttaa agttacctat tcatattcac tgtaaaaatt tcttccacag gaaatgtaat
     1321 tttaaccaat cctattgaga caaactcacc acttattttt ttgaactcac gcagtaatac
     1381 atcacttgcc cgaaaaaaag cctcactttc atcatctcca gtaagtgcta ttgtgcaatg
     1441 gggtacccaa cgattggggt gtaccattcc caaccatttg aatcaaaatc ttgtagtacc
     1501 tcatataatt ctctctgaaa ctgatacata ctgctattca ttataggtga tacaaacacc
     1561 gttttagtat cattaaacat gcctactgaa ccaatgtaag caggtattac cttatggttt
     1621 tgtgcaaacc cctttagctt gtctatgcaa caagcctcat ccacatcatt gaaacaagcc
     1681 aaagtaagat gaggtcttgt tttccattct aagtacttcg tactaatttt ttcatcagcg
     1741 atttttttag ataaatcaaa aagttgcttt tccgtttttt tatcataata tagctctatc
     1801 gcatattgca taataacacc tccaccataa agtaaaaata cctatctata aaaacacaca
     1861 aatacctatc attttaatat tatattacta ctgttcctct tccaattttc tatatagtgt
     1921 agccaaacct atgcctaagt cttttgctat cttcttcttc gtctttgtat ctcttccata
     1981 cttattcaac aacttgttta tgtaagtcaa ctctaattct ttaattgaaa gtatcctttc
     2041 ttgattctct actatccctc caactatatc atcttcaaat attatattta catccttatt
     2101 tttataaata tttccattaa tattataata gtttaaaata tctaatggaa gcatatcttt
     2161 atatattatt ccatcatctc caaccaaatt catcatatat tcaactgcat tttccagttc
     2221 ccttatattt cccggccaat tatagttaat caacatattc attacatttt cttctatttt
     2281 atttaactga atacctaatt ctaaagaata tttagaagca aacttcatca ttattgcctc
     2341 aatatcatcc tttctttccc taagaggtgg caaatttata ggaattacat tcaatctata
     2401 gtataaatca tccctaaact taccttcatt tactaatttc aataagtttt tatttgtagc
     2461 agcaatcact cttatatcta aatctataag tttatttgac ccaattctcg caaattttct
     2521 ctcttgtaaa actctaagta actttgcttg taaaggcatt gacaaatctc ctatttcatc
     2581 aagaaatata acaccttcat ttgcaagttc aaatttacct actttacctc cactactagc
     2641 tccactaaat gcacctttta tatagccaaa tagctcactt tcaaggagat tttctggaat
     2701 agcagcacag tttatcgcaa taaatggctt gttccatctg ctaccttcag catgtatagc
     2761 cctagcaatt agctctttac ccgtaccact ttctccagtt ataagtacag ttgaatttga
     2821 ttttgctaat ttcttagttc tctcttttac ttttaacatt gcttctgaat ttccaattat
     2881 agactctata tcactatttc cccagccact attcactttt ccatgtcctt tatgattgat
     2941 atatgtcttg tcaaatatta ttattttgtc gtattgattt atatatggat ataccggaat
     3001 tattttggca tttatattat aaattttgtt atcaatatca atttctagta attcatgtcc
     3061 atttgaggaa gattcaacag atactatatt tactatattt tctattatat ttttatctat
     3121 atctaatttt tttagagcta tattgtttat ataagatatt ttgctattta tgtctagtat
     3181 tataacgcct ttttcaacat tttcaatgat ttctatcatc atatccattt ttctactgct
     3241 attttttgac gcttcatgtt cttctatttt catagaaatt aaatcgcata tatgattgac
     3301 aaattttaaa taattatcta tttttgcact tatttctact tttttagttt tatctgtact
     3361 aatagcacca attactccta tagttcgatt attatatttt attggaactg ctattattac
     3421 tttatttagg catttcatgt aatgggaaca ttctatacaa agctgattat cacatatatc
     3481 caatacgact aattcctgac ctgtctgtat aacattgtca taaataaatc cttctgtaac
     3541 aacgctttca tttatctttg atttatacag acctgtacca ctaattctta ttaatctatc
     3601 gtcaactatt tcaatatcta catctaacac actagctact gtttcagtgt attttatcat
     3661 ttcttgttga aattctttta atttacttaa catagtttcc cccattagaa tttaatttac
     3721 caaattatca gtttagctat attttaacat atagtcactc tttaaaatat atataaaaca
     3781 ttacttattt atatatttgc gttaaaacag acaatattaa tttataaaaa gtttgtttta
     3841 tatagtaacc ttaattataa tatgcagaca ataaaataaa tcacaataca ataataaaaa
     3901 aaagctttcc tcatataaca aatatatttt tgacataaaa atatacatta tatgagaaaa
     3961 gctcaatagt taaatattta gatagatttt tatatagact tttttaatat aaactttttt
     4021 aattgtagac tttttttatt agtttttaat ttttattagt tttgttattg gttttattgt
     4081 tataatactt ataatactgc cattgcttca atttctaaaa ctgcatcttt tggtaattga
     4141 gcaacttgta cgcatgacct tgctggtgga ttttctttaa aatatgtagc atatacttca
     4201 ttaatatcat taaaatcctt taaatctttt acgaatactg tagtcttaaa tattttatcc
     4261 atacttgtac cagcttgttc taatattgct tttacattat ctaaagaagc ttttgttgct
     4321 tcttttgggt cagatactaa ctcgccagtc ttcatatcta ttggaagttg acctgatgta
     4381 gaaactatgt ttccaacttg aattgcttga gaatatggtc caattgctcc tggagctttc
     4441 tctgtagcta ttactacttt tgacatgact tcacctccta ttattaagtt atatttacag
     4501 gaaaataaaa tttcctaaac accaatatta atacgaacct ttagattaaa tttcaaatct
     4561 aaaacttcaa ataatcaata cttagatgat tatgtttatt tgtgtcttgt taacttttat
     4621 taaagcaatt aacgtgccaa actttattta tatataaaat tttgtgaagt cattcaaaat
     4681 agttacttct gaattttttc gtaaaaaaac gctctaaaat tactttttta tgatgtcaat
     4741 ttttttctca tatctagaaa tttaccaaca tgagaattct tgttttgaga atttacagat
     4801 taaagttatt ttttaacctt aaattctgaa cctcccatga ctaaagtcac agggttcctg
     4861 cttcatagaa ttttgcataa tcaagttatt acatatgaaa gaactatagt tgacaatccc
     4921 actattataa ataataaaac ctttacccat accttattta ccctagttat catagagcct
     4981 attgaaaata taacagctaa atataataat aaccaaaaaa ttatcaatat ctaccactgt
     5041 tgctgaaaat ccaaaaaact accttttgat agtcttactt aacaatctaa ttaatctcat
     5101 aataaattta caacttataa ataaattgtt atattggccg tatctcaaca actttctcct
     5161 ttcctagttt taaatctttt atagaattgt accacgtcta agtaaatttt catactatta
     5221 agtcttattt ctaataaatc attagctctc attctatgcc caataatgta agattgcagt
     5281 aattttattg attttcatag ttttatttaa tatacttaca ttaattattt tcatatgaaa
     5341 ttattattta tataggggga tttttttaat atgagcaaaa gtgaattaac agcagaaaca
     5401 acagaagaaa tgttagaagt actaagtggt aaagattatg atattgcatg tcatttacat
     5461 gaacttggta aatcattaga ttgtaaaatt gaaccaaaaa caggtgctcg ttcttacaaa
     5521 atagtatatt caactaagaa accaaaacgt agcttattta ctattgaatg taatgaaaaa
     5581 aaatggagag ttaaagcaaa tctttttcat ctaaatacat ataaagatgc tgtggaggaa
     5641 tgctctaaaa ctattaaaga tagtataact aaaactcgta cttgtaagaa atgtaattca
     5701 aagtgtattg gaggttcttc gtttgaatta gatggaaagt cttacctgac ttgcatagga
     5761 agtggtcatt attttgcaaa tatggaagaa atggattgga aaaacctaga aaaattaatt
     5821 actaaagaaa ataatattat gcaggaatct gtatagtttt ttaagggtaa ttattaaaat
     5881 atatgagtaa caacagatac ttaaataggc ataaaaaaat aaagggtata ataaggatat
     5941 agaataaaaa taaatgatat agcacaaaga ggctgtctca aaatacagat aagcctcttt
     6001 ttattgttga aatgataaga atatattcaa ttttatacaa ttttatacaa acatactacg
     6061 ccaacttacc catttcatat gcctgtttca ttgccttact tcccttaata tcacctacat
     6121 tccaagcacc tacaccatat actatacctt tttccttaga accatttaga catgatgtaa
     6181 atcctctaaa tccttctatt gttctttcca tatcagccac attttcatct gctgctgtaa
     6241 cgataaaata gaaatcttta tcacaaattt cagtatatct agagcaagtt ctatcaatca
     6301 tagttttcat ttgtgcattc atagtataaa aataaactgg agtcgctaaa acaatcacat
     6361 ctgccccaat cattttttca agaatttctc ccatgtcatc tgtctgtgaa caatttgctt
     6421 ctttttcata acaagcacca catccagtac aatagttaat atctttatct tttaaaaata
     6481 tcttctccac ttgattttta gattctaata ccccttgtat aaactggtca cacaataaat
     6541 cagaatttcc accttttcta ggactagctg atactattaa tacattttta ctcataagat
     6601 actgcctcca ttttcttatt tctactgctc tttaatactt tttctttctc aataatacct
     6661 tcttcatatc tatcaatctt aaaatttaat cgttccaaag ttttttgcat ttcttctatt
     6721 ctttttgcta gttggtttct ctgctctatt aaaagttctt ttctagcttt gatagtactg
     6781 ctcccttgtt gaaacatagc tacatactca attaatgtct caatagaaag acctgcacct
     6841 ctcatacact ttatgaaatt aacccactca caatcttctt ctgtaaaaga cctaattcca
     6901 cttttatttc tatttacagg tggtattaaa ccaattcgtt cataatatcg taaagtatcc
     6961 gctgaaagct cgtatttttt actaacttct gaaatcgtca tacatatctt ctcctcttga
     7021 tattgtatag attgaattat ttctcaaagg tttctgctac attctttaaa gcatctataa
     7081 ttttaatgtg ttgaggacag ctttcttcac attgtttaca ttcaatacaa tctgaggcct
     7141 ttccataagt tttagtataa ttattatagt agaccatctg tgttgaaaat ccagtattaa
     7201 gagcttgttt ttctgcattg tacaacgcaa aatagtttgg aatagcgata ttttttggac
     7261 agccttctac acaatactga caggcagtac aaggaattaa aattgattcg ttgataatct
     7321 ctactgcttc atctataata ttatattcct cttgtacgaa tggtttgaaa ttttgcatat
     7381 atcctgtatt gtctaataat tgttccatat ttgacatccc acttaagacc atcataacat
     7441 tatctttact tgctgcaaaa cgtattgccc aagatgaaat tgacatatct ggattgtagt
     7501 cattcaataa tttctctgct ttttctggta tctttgcaag tgtacctcct tttactggct
     7561 ccataacaat tacaggctta ttatgcttct ctgccacctc gtagcatttt ctagattgta
     7621 tgctttcatt atcccaatct aaataattaa tctgtaattg cacgaagtct acttctggat
     7681 gctcactcaa gactttatcc aataattctg cactatcatg aaaagaaaaa ccaatattct
     7741 ttatttttcc ttcttttttc ttttcttcaa taaacttaaa gctatcaaat tttttagcta
     7801 cttcgtaatg tgacacgcct atattgtgta aaagatagta gtcaaaatag tctacaccac
     7861 atttttctaa ttgctcatta aaaatggttt cttgctcttc ttttttcttt aaagccatta
     7921 atggaagttt tgtagctata gtaaaactct ctcttttgtg tctttttact agagattctc
     7981 taagtgctat ctcacttttt cccatgtgat acacataggc cgtatcaaaa tatgtaaatc
     8041 ccctttctag aaaagtgtct accattttat ttaaatgttc catatcaata gttgtaacat
     8101 cattttcatc ttttattggt aaacgcatta atccaaatcc taattttttt gcctccattg
     8161 ctttacttcc ccctttaaaa cttgtttgtt ttttctctct actttaacta ttcttacaag
     8221 tatataattc ttgtataatt atactttact acttggagtt aacttcaagt caatagattt
     8281 ttttattttt aagacaaata ttataaaaat acagttcttt aatattaatc cattacatgt
     8341 ttctttcacc ctccaattct caaacttcac aaactccaac atgtaaagaa atatatactt
     8401 taagaaattc agtccattaa tattactaca aatacttttt agatatgttt aaaaagaaat
     8461 ttcacctaaa agatttagaa aagatttaga aataggtctt gtgtctattt taaaatatat
     8521 aaaacagtgg taaagactta tttataagat aaaaaatgaa tagtgtatac cctttttaaa
     8581 aagttttttc ctttttatat ttgtgataca atgttaaata taaaaaggaa aattttgtat
     8641 taaagaaatc ttatttgttg attttgtaac ttcaaactca gcatcaattt gattttaaga
     8701 ttatatttta ttaagtatgc taaataacaa atttttaaag attgtgaggt aaaaatatga
     8761 ataagaaaaa tttagctata ttaatatcag gtatgatgtt tttttctagt tcttcagcaa
     8821 tttttgcaga caatactaca aagcaagaaa gattaatagg taaaacaaga tatgaaacag
     8881 cagtagaagt aagtaaattg ggatgggttc aatcaaaaac agccattata gttaatggta
     8941 attctattca aagtgcgctt tgtgcaaatc catttgctaa gctaaagaat gcacctatac
     9001 ttttagtaaa caacaacagt attgaaaatt caactaaggc tgagcttaaa aggcttggtg
     9061 tagataatgt gtacattgta gatagtggaa atagcatttc ttctaaagta gaaaatgaaa
     9121 taaaatcact taacataaaa ataaataaaa tagtaggaaa caatatatat gaaatgtcta
     9181 caaatgtact taaagaaata gataagataa aaaaggtaga aaatgttgca gttgtaaatg
     9241 gactaaaggg actatctgat gcagttagta tagcttctcc agcagcaata cataatatgc
     9301 ctataatatt agtatcagat gaaaacagca atgatagtag tataaatttc ttaaacaata
     9361 ggtctataaa aaaatcttac atagttggag gaaataagat tttatcagac aatacagtaa
     9421 agaaatatcc aaacagagaa agaatcagtg gttcagatag aaacaatact aatgcaaaaa
     9481 ttattgataa attttataaa gataaaaaaa taaataatgt atttgtagct aaagatggta
     9541 ttaaccaaga aaatgcatta gtagattcat tatcaatagg agtactagct gctaaagaaa
     9601 attctcctat aatgatatct aatggtagcc ttgtaaatag tcaaaaagaa tttattaaaa
     9661 ataaagatat agatgaaata acacaagtag gaggaggata taatcaaaaa gcatttgaag
     9721 atattttaaa actaaaaaat gtgaacataa gaaataaaac taaaaaaata tctttaatac
     9781 aggctaaaga aatattaaaa caagctcttc caggaagcaa tattttagtt tgttattatg
     9841 atgaagatga taatgaatat gactgcaaag ttataaagga taatatccta tatgagatag
     9901 aaattgatgc ctactctggt aaaataatag aaattgaaaa agatgatgac ttaaatgata
     9961 ataatttagg tacacaaaat atatcattgg ataaagctaa atctatagca ttagatgcca
    10021 ttcctaatgg taatttaatc tattgtaaat atgatgctga agataacgaa tatgaggcta
    10081 aagtaataaa aaataagaaa acatatgaat tagagataag tgcatttgat ggaagaattg
    10141 tagatattga catggacaat gattctgatg atgactttga tgataatgat aagaataact
    10201 ctaatactaa tgacaacagt tctattatta gtgctgaaaa agctaaatca ataatgctag
    10261 ataaagtgcc tggtgggaaa atagtaaaat ttaagtatga tgaagatgac aaagaatatg
    10321 aaggtgaaat aataaaagga aaaactgaat atgagataac aattaatgca attactggta
    10381 aaatattaga atttgaaata gatgattaat aaaaaatata tattctatta attattacaa
    10441 gcaataaagc cataaatatt gaaattttca atatttatgg ctttatttta tttttttaat
    10501 ttaaatccac attatgccat tatatatatc tatgcactct tgtaaaatgg gttatatttc
    10561 tccagtatat taaaaaaagt gagtgtctca aaataaatct tctaactcat aaagcactct
    10621 cctttgtatt aaatcaaata tattttcatc atttaaacaa ttaagaagag acttctctct
    10681 attttgagac aactccattt taatacacta tttatacata aacatatttt aaacaactaa
    10741 aaagcaacta atatcttaat aaatatctca atacatatct tcataacaca tatcaccata
    10801 attacaagca gataaagaac tctcctcatt taaatacgac tcaaattcat acccaaacag
    10861 aattgttggt tctatatata tttccatatc tgtatatctc catcttctta cctttgcctc
    10921 tataaccttg taaaaatctt ctaaactata cccttctttt aatcttttat ctataagtgc
    10981 cttagttata ctcaaatcag ctttaaaatt ttttcctgat tcactattta attttcttat
    11041 aataatttca tgtatgcttt ctggattttt gtaagtagaa tcttcagtca aatcttcatc
    11101 cgaattttca aaggtatttt tatttaaatt ctttttattt aaattatttt ttaataattc
    11161 ttttttcttt gtctcatttt ctgtttcata cccagtatga attatcaaat cataatcttt
    11221 gtctatactc tcttgattta tacggctgta tatgttggta ttactgttct taaacaactc
    11281 atttctatgt ttatatttca ttgttttttg gttattgtta atagttgcat caatgtattt
    11341 ctctatattt ttgaaaatat caccattctt tgttacaatt tctgcattac tactttcaga
    11401 acaaacataa ctatatacag aacaacaatt tccctttact cccctactta ttaatctcaa
    11461 tatacctaaa tctataaatt catttacaag tctacttatt gtagatatag atacatcaag
    11521 gtcagatgca gctttcctta ctgtcatcat aaattgtcct cttggcagag ttccttttaa
    11581 tctttgtata ttttcttttc tcattacata gtgattaaac cttattctat ttacatctct
    11641 tttttcatta aaatataata ctgctgaatt taattgataa aaactattac tcatattccc
    11701 actcctccaa tttaaataga taatttgttg taaagttttt agatttttta ataacttatt
    11761 aaatgattat ttttattaaa aagtattctt tgatattttc attttaaagt tttaagtata
    11821 ataagtt
//
LOCUS       DAEOSP010000123        11765 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.124, whole genome shotgun sequence.
ACCESSION   DAEOSP010000123 DAEOSP010000000
VERSION     DAEOSP010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11765
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.124"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..985)
                     /locus_tag="KRH54_002490"
     CDS             complement(<1..985)
                     /locus_tag="KRH54_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8580141.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNN"
     gene            complement(972..1739)
                     /locus_tag="KRH54_002491"
     CDS             complement(972..1739)
                     /locus_tag="KRH54_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580142.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            2027..2701
                     /locus_tag="KRH54_002492"
     CDS             2027..2701
                     /locus_tag="KRH54_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8580143.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            2725..3729
                     /locus_tag="KRH54_002493"
     CDS             2725..3729
                     /locus_tag="KRH54_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8580144.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            3841..5070
                     /locus_tag="KRH54_002494"
     CDS             3841..5070
                     /locus_tag="KRH54_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBG8580145.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            5078..5752
                     /locus_tag="KRH54_002495"
     CDS             5078..5752
                     /locus_tag="KRH54_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580146.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            5755..6987
                     /locus_tag="KRH54_002496"
     CDS             5755..6987
                     /locus_tag="KRH54_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8580147.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(7273..8538)
                     /locus_tag="KRH54_002497"
     CDS             complement(7273..8538)
                     /locus_tag="KRH54_002497"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBG8580148.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(8591..9739)
                     /locus_tag="KRH54_002498"
     CDS             complement(8591..9739)
                     /locus_tag="KRH54_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBG8580149.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(9839..10384)
                     /locus_tag="KRH54_002499"
     CDS             complement(9839..10384)
                     /locus_tag="KRH54_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG8580150.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(10607..11551)
                     /locus_tag="KRH54_002500"
     CDS             complement(10607..11551)
                     /locus_tag="KRH54_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBG8580151.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
BASE COUNT         4193 a         1822 c         1476 g         4274 t
ORIGIN      
        1 cgttatttac ataagaattt cctaaattta tggtataact gtatttataa ttttcactta
       61 tgttcctatc tatatcgtaa taaagtgata tggtaaaccc tagcatagtt acacttgtag
      121 ctattaatat tgctattaca gccaatacac tactattgct ctttaattta aatccaagtt
      181 cagaaaatgc aattaaattc tgtcccttat aataaaaact ttttctcttc ttaacagcat
      241 caataatcat atacatagtt gatgaaaaaa ataaaaaagt tccaggtatt attgtaacta
      301 atataaaaac tgaaagcata atattttcag caataaaata actaattgac attaaataag
      361 aaatagctat taataaaaca ccaagtacac attttaactt tataaatttt ttaaacttat
      421 acttttctgg atatttatta aataactcta taattctctt atttctgata actatagtat
      481 ttcttattcc aataactata aatattatta aaaatacagc taatgtctga actaaggcct
      541 ttatacttaa gttcatattt actactatca tttcattcat taattttaca agcatcatag
      601 tcataagctt tgaaaataac agtcctaaaa atatacctat tagtattgca actataccca
      661 tcaaaaaagt ttcatataaa aatatttttc ctatctgatt tttcctcatt ccaagtaaac
      721 tataagttcc tacttctcgc ctacgtctat tgaaaaaaaa tgagtttgaa taatatataa
      781 ataaaaatac aaataccact attacaattg aacttgtatt aatactcatc ctggttttct
      841 ctccaagtga actcaacgct tcattatact ctatagattt aaaactaaaa aatacaaata
      901 cactaaatac aattgaaata aagtacatta aataattaaa aaaactattc tttacatttt
      961 gcattgcaat tctaaataaa gtcattttta ttaactcctc caattaagga aactgtattt
     1021 actattttat tatagaactc tctgttactt tctcccttta caatttctgt gaatattatt
     1081 ccatccttta taaagagtat tctattgcaa aaactcgcac ttatagcatc atgagttacc
     1141 atcaatattg ttttattgct gtctttgttt aattttaata gacaattcaa caaatcccta
     1201 gcagatttag aatctaatgc tcctgtaggc tcatctgcaa gtactaatga tggttttgtt
     1261 gctatagctc tacatacagc tcctctttgc ttttgtccac cagaaacttc gccagggtat
     1321 ttatctaata tagattctat atttaattct tttgaaatct cttttattct attttctatt
     1381 actgatactc tttcattttt taaagaaaga ggcaacatta tattttcttt tattgtaagt
     1441 gtatccaaaa gattagaatc ttgaaataca aagcctagat tatcacgtct aaaatctgcc
     1501 aattcatttt gcttcatttt aatagtattc tttccattta tataaatatc tcctgatgaa
     1561 ggtaaatcta ttgtagaaat aatatttaaa agtgttgatt taccagcacc ggaagacccc
     1621 ataataccaa caaattctcc tttataaacc ttgaaagtta ttcctcttag agcttcatgt
     1681 tttgaacctt ttcttccata ctcctttttt atattttcaa cagataatat ttcttccata
     1741 gttaataatc acctttcatc acaattagtt atttatcaat tctcacaaat atattatata
     1801 tattaaaatg gcttttaaac atttaattat attaatttaa ggttacattt tcgtaagtat
     1861 taattatatt tcttcaaatt tattgcaatt atgtttaaaa tataatttat caagtaaaat
     1921 atagttatat taatataatt attttaggta ataaggtaga aatattttaa tttatcaagt
     1981 agaatatagt tatgttaata taactatttt gaattaggag gtaagaatgt ttaaaataat
     2041 ggtagttgaa gatgatgtct cattgaaaaa tatcatagca aagtgtctta ctaaatgggg
     2101 acatgatgtt catcaaatag aaaatcttga aaatataata gaagagttca aaaactataa
     2161 cccagaatta gttttattgg acataaacct tccattttat gatggttttc attggtgtaa
     2221 tgaaataaga aaaatttcaa aagttcctat aatttttatt tcttcaagaa attctaatat
     2281 ggatgtaata atgggtgtaa atttaggtgc agatgattat atacaaaaac ctttttctgt
     2341 agatgtctta gtagctaaag ttaatgccct tttaagaaga acttataatt ttgttgataa
     2401 taactcaaat cagattattc acaatggtgt tactttagat ttatcaactg caacaataaa
     2461 ctatgaagat aatactattg agctaacaaa aaatgaaatc aaaattctcc atgagcttat
     2521 gaaatataag gggcagatag taagtaggaa taaactcatg aaaaaattat gggataatga
     2581 ttggtttgta gatgataata cattaactgt caatgtaaat agaatacgct caaaattaaa
     2641 tgaaattggt ttagaagatt ttatagaaac aaaaagagga ttgggttata taatcagtta
     2701 aaacaattct aagtaggtga atttatgagc tttaaagatt ttctcaagga taaactaggt
     2761 tttttagtat acaattttac tttcttaatt tttacagtat cagttattat attttctcct
     2821 gtagatgttt ttctgactga tactatttta tatatattag ttgtaaatgt tgtgttttta
     2881 tttttatatc tatttattag ctacatcaag aagaacaaat ttttagataa gataaaaaat
     2941 gatacattca aaattaatat gaatgatata gaattagcta gatgtaaaaa tgaagaaaaa
     3001 atatacttga acattattaa aaatcatcaa tatcaatgta ataacataat aaataataat
     3061 atcgagaaat ttgaagaaaa caaagagatt ttaaatatgt gggtacatga tgtaaagata
     3121 cctatagcta taataaaact cattcttgaa caaaatgaaa atttaattga tgaaaaaatt
     3181 tcagatgaca tagatagtga aatatttaga attgaaaact ccgttgaaaa aattttatat
     3241 ttatcaagac tcgaagattt tcataaggat tttttaattc aagaggtaaa tattgaaaaa
     3301 atcattagaa atattataaa aaaatactca aaatatttta tttcaaaaaa aattatttta
     3361 gacttaaata atttgaattt taaggtttta tctgatgaaa aatggctata cttcatattc
     3421 gagcaattat tgagtaattc cctaaaatac tcctcattag gagataatat atctatctat
     3481 tgcaaaagaa ccaaaaatta tttacaatta agagtggaag atactggttg tggtataaaa
     3541 aaggaagatt taaatcgaat attcaataag ggatttacag gtaataatgg aagaaacaat
     3601 tctaaatcta caggtcttgg gctttattta gtaaaagaac tttgtgataa gcttggtcat
     3661 aaaattgatg tagattcaga gtacaaccaa tatacaaaat ttactattac attcaaatgt
     3721 gatttatagt atcttattat tgatagttta ttttcattaa cttatattca acttcttata
     3781 gtaattctat atgtacatcc cagtatataa ttaagtagtt tttataaaaa ggaggaagac
     3841 atgtcttttc taaaaaaatt taaaaataaa agatttagaa ataacagttt taaaagtaac
     3901 aactttagaa atagcagttt tggaagtagt ggcttcaagt ctaaaaataa aggttttaaa
     3961 aacaatccct ttaaaaataa gagattcaaa atatatgcag gaatagcagt aatcatactt
     4021 ttaattctag gagttcttgc ttatgttgat tcgaaaaaca ctgaattaca agctaatgaa
     4081 aattttatcg aaacatatac tattcctgaa aatgaaaaaa tatttataaa tgggatggtt
     4141 gttcctaagc aaactaagga cttcaatata tctggagatt atgagttaag tgatgttaat
     4201 gttacaaatg gtcaaaaagt aaatcaagga gaccttcttt ttactgctaa aaatccaact
     4261 ataatagcag aaatagatag tttaaaatca caacttagtc aatacaaaaa acaaaaaata
     4321 tcactttctg atattgctga aaataaagat gctattgctt ctataaatgc acagataact
     4381 gctttaaact ctcagatagc ttctctggat aaaaaagctt atgacagagt tactgctcct
     4441 tttgatggca cagtatactt gaatgaccaa actggaaatc cagaccaacc agtttcattt
     4501 atgactattc aaggtctaga gttttatatg aaaggacaag caagtgaaca agatttacct
     4561 aaaatgaaga ttgaccaaat ggtcaatata ctagtttttt caactgacca aaagcttact
     4621 ggtagaataa gttttatatc tgataaacca tctactccaa atactgaaat gggagctcag
     4681 cagaatacac tatcttatta tgatataaac atagcatttg ataatcaaga aggattagta
     4741 aatggattcc atcttcaagc atcacttgaa gtagctaatt caagctttaa aataccagca
     4801 tcttgtgtac ttaaagacaa caaacactca tatgtattta aggatttaga tggtatattg
     4861 aaaaagcaaa ttgtagatgt agcaagtcaa aatgatgatt ttgctgtagt tagaggcggt
     4921 cttgaacaag gcgatataat aataaaacac cctactaaag aaatgaaaga aggagaccca
     4981 gttcaaggcg atggcgtaac tgctggttct aataatggag atactactcc aaacaaaaaa
     5041 gtagaagaag ttactatgga tgtgaattaa tttacatatg ttaattaaat tagaaaatat
     5101 tcaaaaatat tacaaagtag ggaaagatga attacatgtt ttaaaatcct taaatttaga
     5161 aattgaatct ggagaatttg tcatgataat gggtaagtct ggtagtggta aaactacttt
     5221 acttaatatc ttaggttttc tagatgtatt tgatgaagga agatatatat ttgatggaac
     5281 agatgtaact aatttgagtg aaaatgaacg ttctgttttt agaaatatta atattggatt
     5341 tgttttccaa caatttaatt taatagaaac tctaaatgta tatcaaaatg tagagttgcc
     5401 tctaatttac aataaagcac taaaaaaatc taatagagaa gaaattgtta aagataaatt
     5461 aagttctgtt gggcttttgg acaagcttaa gcagaaacca cttcaattat ctggtggtca
     5521 acaacagcgt gtagccattg cacgttgcct agcaaatgac cctcagataa tctttgctga
     5581 tgaaccgact ggggcattag atagtgaaac tagcagagaa attatggagc ttttaacaag
     5641 gctcaacaaa caaggtaaga ctataataat ggttacacat gatcaagatt taactaagta
     5701 tgcaactaag gttatacgac ttaaagatgg tgtgtttact tcggaggtgt aatcatgagt
     5761 ttattaaaaa attctttggc caatttaaaa ggtcacaagc ttcgtgtatt tgtagcttta
     5821 ctctggatta taataggaat aacttcagta atacttgtta gttctatagg taatggattt
     5881 caaaaagaaa ttaagaaatc tgtaaacaat gtaaacccta ataaaactac tatttcgttt
     5941 gaatcagccg ataacacagg tcttactgat gatatgagta tttttttaaa accctttaat
     6001 gcaaaagatt tagaagaatt atcatttgtt gaaggagtag aaagaatagc tccttcaaga
     6061 gatgggttta accttgattc agtttactct tcacaagcat cttttgataa aaaaactacg
     6121 tatgttgatg ttggaccagt aaaaaaagat tctaaaataa atttaatttg tggtagagat
     6181 ttttctttag atgatgaaaa aagaaaagta attttactta cattacaaag tacaagtgaa
     6241 atttttgaaa atccagaaga tgccttaggt catggtatca acataaatgg aactatattt
     6301 gaaataatag gggtattaga tgattctcag caaaaccaag ctggaggatt ctttggaggt
     6361 taccaagata tgcaatttac tacttcccta gtacctaaaa aggcttttga cactttaatg
     6421 agtcaaaatt cttattcaaa tgaaatttac cagttagact tagtttcttc taaaggctat
     6481 aatgtaaatg aagttgcaaa caatgttata gctaaattat atgagatgca ccctggtatc
     6541 aatggttctt atactacacc agacccaact gaacagacag cttatcttga aagcattaat
     6601 tccaatgtaa acaaatatgt atctataata actgtagttg caatgtttgt tggtggtatt
     6661 ggtgtaatga atataatgta tgtatctgtt atggaaagac aaagagaaat tggtataaga
     6721 cgtgctattg gagcaaaacc taggtctata ttatttcaat ttttagttga agctgttttt
     6781 ataacagtat gtggtggaat tttaggaact atagttggat ttgctgctac taactatgtt
     6841 tctaaatata taggttttga agctatacct agcttaaata gcttatttta tgcaattgta
     6901 gctactatat tgactggagt tgtatttggt ctgattccag cctttaaagc ctctaaacta
     6961 gaccctataa aggcaattta taagtagaaa ttgtataaag attttcgatt tttagattag
     7021 tttatataaa acatacttaa atatataacg cactattata tatgatagat taggaactta
     7081 atctttccaa atataatagt gcgttctttc ataaaaagga ttctcttcct tactatatct
     7141 aaattaccaa gatgttatgt tctaattgtt caaactaata cttccaattt tgattaatca
     7201 atctcattct cctaatatac aatcttttac tttcaatcat tgtacactcc gccttaaaaa
     7261 aataatctta aattatgcgt tatatacaat ctccccatta catattgtat aattaacctt
     7321 tccatataaa gatttacctt taaatggtga attatcagct ttagaaacaa agctttcaac
     7381 tgtccattct tcttctggat tgaatataac aatgtctgct acagcccctt cttctataaa
     7441 tccaatattt aaattgtaca actttgcagg atttatagac attttttcta ttaactgcat
     7501 catactaaga tgatttttat gcaccaaatt tgttattcca agagctaaag aagtttcaag
     7561 cccaataatt ccacttggag cttttataaa ctctctatct ttttcttctt tactatgagg
     7621 tgcatggtcc gttgctatta tttcaatagt accatcattt aatccctcta taatcttatc
     7681 tctgtcccat ttacttctta gaggtggatt catttttgca tttgttccaa actccaatac
     7741 atcttcctca gtcaatgtaa agtgatgtgg tgaagcttct gcaactacat ttgcacctaa
     7801 gtattttgca aatctaacca tatcaacaga tacacctgaa cttatatgtt gtatatcaac
     7861 tttagcacca gtctttagtg ccaacataca atctctggct accataacat cttctgctac
     7921 acttgatgca ccttttaatc ctagttctga agctaccttt cctgcattta cacctggatt
     7981 tccaactaaa gaagggtctt cttcatgaaa actaagtggt acatccaact cttttgcctt
     8041 aatcattgcc tccataatta gattgctatc cattaaaggt aatccatcat cagtaaaccc
     8101 tggtacacca gcttttttaa gagcttccat atcaactaac tctttacctt caaaaccttt
     8161 agttatagct gctgcttgta aaacatttat acatgcattt tttgactttt ctttaacata
     8221 attaaaagta tcttcattat ctacaatagg attagtattt gccatacata taacagttgt
     8281 aaatccacct ctagcagcag atttagctcc actttctata tcttctttat atgtaaaccc
     8341 agggtctcta aagtgtacat gtacatccac taaacctgga gcaactacat taccactagc
     8401 atctattatt ttttcatatt catcactttc atcaaacttg cctattttat aaattttatt
     8461 ttcttttata attaagtcaa tattctcatc tctttgactt ttagggtcta ttactctacc
     8521 atttttaatt aaaatcatat tatttctcct ttatattttt aatttatata gactcatata
     8581 atattataca ttattttaca tacttaaata caaaattacg tttgacatca atagcctttt
     8641 taggacaaag ttcatggcaa cagtaacatc ttatacattt acttaagtct ataatagcac
     8701 ccttagtccc tatctctata gtggatgcag gacaagcttc cttgcatttt ccacatttta
     8761 tgcaatcttg aaatcttacc acaggctttg gtgttataag ctttgaaata ggttcattta
     8821 aaaattttgg tagctttaca gaaaacaatc ttaaatcctt acttacattt ggaatcttgt
     8881 aattttttat aattaaatct tttatatctt cacctaaaat ttcaatatct gaaaaatctt
     8941 cttgtatata tcctctctta atagcacctc taattgtagc aactctattt ggaagtaaac
     9001 ttataatctt acatgcaaca acatctatag catatggatt ttgtgatgct aataaaactc
     9061 ctatttctcg agtagtacca gctgaaggtc catcaccttc catacctact ataccatcca
     9121 taattgtcaa tgttggtttt atataatcac aaatatctaa aagtacattt tcgcaaaaca
     9181 actcttctgt aggaaatcta taatgtatct ctgctttttt caatccagct atgcatccat
     9241 ataagttttt tgtaccacca gtaaaagttg ccattatatg agtctttaat ttacaaagat
     9301 ttattatata atctgaatct actattggtt taataatatc catatatttt agaacatgtg
     9361 ctttttcact atgtatctta acatctgaaa catcataatt taacttaatg cccagttcat
     9421 ttgctacttc ttcaattcca gatgccttat aaatgctctt aagagaactt tgattatatg
     9481 gaccaccagg gctatctgct attgtaatat cacaatttaa ctctaaaagt ttttcacata
     9541 caacttttac aaccatagga tgtgtagttg tagcttcttc aggctttttt ttcattaata
     9601 aatttggttt tataagtact ttgcttcctt ttttaacaaa cttatctata ccaccaatat
     9661 cctctaaatt tttttcaata gataacttta cttcttcata attgtaagtt ttacaactcc
     9721 taacagatac cgacttcata ttatctccat ccttctatca caacagttta tttgtataat
     9781 ttaaaaaagg agaaaccaaa gtttctcctt ttaatctatg ttttaattgt tttaattatt
     9841 attttccaaa atgtctgttt aataatccta agaatgcttt accatgtcta gcttcgtctt
     9901 tacacatttc atgtactgta tcatgtatag catctaatcc taattcttta gctctcttag
     9961 ctaattttaa ttttccatca gttgcaccat actcagcatc aactctaact cttaagtttt
    10021 cttttgtatc tgcaactaca acttctccaa gaagttctgc aaatttagca gcatgttcag
    10081 cctcttcaaa agctattctc ttatacgctt cagctacttc tggataacct tctctatcag
    10141 cttgtctact cattgctaaa tacattccta cttctgtaca ctcaccagta aagttagctc
    10201 ttaatccttc gattatttct gcatctacac cttgagctac tcctattcta tgttcatcag
    10261 cccaaaccat ttcgcctttc atttcttcaa atttatcagc tccaacttta catactggac
    10321 attgtgctgg tgcagcatct ccttcatgta tatatccaca tactgtacaa acaaattttt
    10381 tcataattaa tttcctccca attttatata attaaatttt tatttttaag tttaataact
    10441 tgctacaaga atatatatac cccagacttt atagcttaaa cactaaaaat aaaatttatt
    10501 tcctaattaa ttttatcact ttgaaacacc agtatcaacc aagttttgat gtcaaatttc
    10561 tacataatac ataatttatt tgattaatta ggaaatagtt gtcatattaa atactttcta
    10621 atgaagactc ctctgaatct ttaagtttca cttttttgct ctttaaaaaa gataatttag
    10681 tttctgcagt tatgattgca acaaatatca agatactacc aataaccaat tttgctgtaa
    10741 taagttcctt tagaattatt atagaaaata ttgtaccaaa tacagcctct gtggataata
    10801 ttatagctgt tctagttcca tcaactcttt tttgacatat agtctgaaat aaaaatccaa
    10861 ttgtagtact aaatacagct agatacatag ttcccatgta accagataat tcagcttcca
    10921 tctttaattg acctgcaaat gtttgaacta caacagacat taaaaacgct actgtaaatt
    10981 gaatagctgt caatgccatt gggttgttat cttttgcaaa ctcacttgta aagaatatat
    11041 gaaatgcaaa tccgaatgaa catataagtg ttagaaaatc tccaaaatta atagagaaat
    11101 cagcttctaa agaaagtatc ccaattccta taagagctac caaactactt attattccta
    11161 ttttatcaag ttttcttcta taaagtataa atcctatgaa tggaactatt acaacattag
    11221 ctgctgttat aaacgcattt ttagatggtg tagtgtacat aagtccaata gtttgtactg
    11281 tgaatgctag aaataaaaat attcctaaaa tccctcctgc ttttaataat tttttaccca
    11341 tattggcacg aatctgttta aaaaacagta aattcataat tatagctgcc aagaaaaatc
    11401 ttaaagttat gatttgtaga ggtgtaagcc caccgtctaa tgcaaattgg gttgctacaa
    11461 agccacttcc ccatataatt gctataaata ttagtcctat ttctccaaag tattttttca
    11521 ataaagcccc ctccttttta tttttttgca aaattataaa ccccctaaac agcaagagag
    11581 tttttctata agcattacta taaattaata tagtaaattt tttcagtatt ttaaatctaa
    11641 atttacatac attttgagct caaatttata tccactattt aacagtaata tattaatcat
    11701 tttttgtaaa caccttttta ataaattttt atatttttta tattttttta cctatactaa
    11761 atttt
//
LOCUS       DAEOSP010000124        11643 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.125, whole genome shotgun sequence.
ACCESSION   DAEOSP010000124 DAEOSP010000000
VERSION     DAEOSP010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11643
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.125"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(107..1020)
                     /locus_tag="KRH54_002501"
                     /pseudo
     CDS             complement(107..1020)
                     /locus_tag="KRH54_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
     gene            1276..1632
                     /locus_tag="KRH54_002502"
     CDS             1276..1632
                     /locus_tag="KRH54_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8580152.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            complement(1747..1923)
                     /locus_tag="KRH54_002503"
     CDS             complement(1747..1923)
                     /locus_tag="KRH54_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580153.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     regulatory      complement(1976..2065)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      2685..2784
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2915..3205
                     /locus_tag="KRH54_002504"
     CDS             2915..3205
                     /locus_tag="KRH54_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBG8580154.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     gene            3180..3950
                     /locus_tag="KRH54_002505"
     CDS             3180..3950
                     /locus_tag="KRH54_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8580155.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSIHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            3972..4985
                     /locus_tag="KRH54_002506"
     CDS             3972..4985
                     /locus_tag="KRH54_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG8580156.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKNNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            4954..5688
                     /locus_tag="KRH54_002507"
     CDS             4954..5688
                     /locus_tag="KRH54_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580157.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            5849..8692
                     /gene="mutS"
                     /locus_tag="KRH54_002508"
     CDS             5849..8692
                     /gene="mutS"
                     /locus_tag="KRH54_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBG8580158.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            8703..10670
                     /gene="mutL"
                     /locus_tag="KRH54_002509"
     CDS             8703..10670
                     /gene="mutL"
                     /locus_tag="KRH54_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBG8580159.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYFVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNIDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            10708..11643
                     /gene="miaA"
                     /locus_tag="KRH54_002510"
     CDS             10708..11643
                     /gene="miaA"
                     /locus_tag="KRH54_002510"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBG8580160.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEAKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
BASE COUNT         4741 a         1267 c         1882 g         3753 t
ORIGIN      
        1 atatatatta acaaataata aaaaaattaa tttgattgta tataaattag tttgttatta
       61 aattagtatt aaaaagttca aaaatttact tagaaggaat ctataattag atttctatgt
      121 ttgttgagaa tgcaattaca gcttttccaa ttatttttac ttcaactggt ttattatcta
      181 aaataattac ttttactaac atcttgcctt tgcgttttat tgtttctcct tgaactgctt
      241 caaatataaa ctctgtgttg ggtgtaggta ttactttata atgtacaagg tacgcaccta
      301 agggtccatt agcatttcct gtaacagggt cttctgaaat accaattgca ggagcaaaca
      361 ttcttccatg aacttgtaga gtttctttat tatgtaatgt aaaaatataa tatccattac
      421 attttatttc ttgacttatt ttcgataatt catataaatt tggagataaa ctatttaatg
      481 ttgataattc ctttattcca atcataactt ttgaatgtcc tgtagatgat atggcaatag
      541 gacaatctgt acgaatatct tcctttaaaa taccaaatgc attaagtaat cttttttgat
      601 aggtttgttg taacggtgga gatacttcaa tttttccttg tgtcataatt atttcataat
      661 cattatcttt ttttataata tcaactggta atattccagc actagttttt tgaaatactg
      721 ttttgttatt gaattggttt tctactgctc taacataatg ggcagcaata gtagcatgac
      781 cacatatagg aacctcatta gttagtgtaa aaaatctgat atgtacatca taattttctt
      841 gatgattttt agccaagtta ttgaaaataa aagcagtttc tgaattattt aattctcttg
      901 ctattttttg catttgtgat tcagataagc catcagcatt tgtaattaca ccagctggat
      961 tacctgtaaa tttttctttt gtaatgagtc aacttgataa agacgaaatt ttttattcat
     1021 attatataat cctttctaaa cattagatta gtttatctac atagaaaact tgagataaat
     1081 tattatttat tattatctgt ttaaataaaa ttaataaaat aacatatgtc tatattaaag
     1141 tttaaacata tacatctaaa aaagtagtat tatgtaaggc tctatactac aatcaagatt
     1201 atattacgaa ttagtacgct aaacaagaat gcacttttta gttagatact tactattttg
     1261 aaagagagga ttgagatgga tatagataat aattttgaag aatttccaaa tattgaagaa
     1321 agacaaaaag attttaattg ttcaattggt tttaccatga ctgtgatagg aagtaaatgg
     1381 agggcaatta ttttatggca tattttaaaa caagagccaa tacgttacag tcaattaaaa
     1441 aaagaagttg ttcatataag tcataagata ctgtcacaag aattaaagta tcttgaaaga
     1501 gatggactta ttaagaggat ttcttatccg actattcctc caaaagttga atatttgtct
     1561 actaaacgtg gtaaatcatt agaatctata ttattagaat tatgtaattg gggtaaacag
     1621 tatatgagat aaactcaact gtttacttta tgaaaaatat ataatgaagc ttattttaat
     1681 tagttcttta atgaattata aaacgtagat aaaactaaaa agggttttct agaaaataaa
     1741 atttctttaa ttatatctgc ttaccaatac catcacttga tgcaaacaag attctaccac
     1801 attctctcca tattctagta taaaatcatt taattgtgta tctataaaat tgcagtaatc
     1861 gcgtgaactg catatatatg cagttagtat atgtgtacat ctatccattt tatccataaa
     1921 catatatcat atatccccct aaaaaacatt cataaaaaca aaaagaaaat atctctttgg
     1981 caactggctg acataactca tttcataagt cttagtccct atagctttgc gtcactaaat
     2041 ttctctagtt ttgcctattt agttttattc tactattacc ataatatagg aaaacgtttc
     2101 ttttttcaag gtgcatatta aaaaatttaa aaattaaaaa aatattttat ttctcaaaaa
     2161 taaattggat aaaataatta tattatagag tttgattctt atctaaaatg ttggttgctg
     2221 aggattttga tgataatgat gagtaatttt tatattatca caaaagatac ttcctaaaag
     2281 gcttgtatct taaaaatcta gatagaggtt tatttttaga ttactgaata ttattaatga
     2341 atttattgaa ggattttttg tagataaaga taagtagagt ctaaaagcta tttctaatct
     2401 gtaatttgta acgattgcaa attagatgag tgaaaacaga aatttagcac aatatttgta
     2461 tggaattgat atacatccaa atatgcttga aatagcaaaa taaaaactag aaaattcagt
     2521 taatctagtt ttggaagatg ttgataaatt accattttaa gataatttat ttggtgtaat
     2581 aatgtacaat ttatattaga tatttttgaa cggataaaat atctaatata aataaaaaat
     2641 ttgttaaaat atgataagtg tagtataatc aagtagaaaa gatataatat atttcgggga
     2701 gctaatgtgc tgagaggaga ttttatctcg accctttgaa cctgtttgga taatgccagt
     2761 gtagggagac cagttaagat aaaaataagt cttatcctat tttggataag acttttcttt
     2821 tatttaaata taatatcttt ttagtaataa aattttttaa taaaaaacaa aatataccta
     2881 gtaggtatta attattttta aaggagtgat ttaaatggta atcgcagaca tagcagtaat
     2941 gccactaaga ccttacaaag gtgaagaaga gatgtataaa gtagtagatg cttgtattgc
     3001 tgaaattgaa aaaagtggtc ttaaatgtga aataggagca atgagcacta ctgttgaagg
     3061 tgattttgat gaagtttttg aattattaaa aaaggtacat aaaatccctt ttaatcttgg
     3121 atgtgaaaga gtcataacag tagctagagt agatgaaaaa gctggaggtc tgactataga
     3181 tgaaaaactt agaaatcata gataaagtca aagatagtat atatccaata gcaacatttt
     3241 tgtttatact tattctttgg caatctatgg taggagtttt agaagttcct cagtatatac
     3301 tacccacacc agtagatata attaatgtgt tttttaaaga ttatcaaaat ttatccatac
     3361 atgctgtggt tactatagga gaagcaatac ttggttttat agtcgcaatt ataatatcat
     3421 tggtaattgg aatattgatg gattttgtat caataataaa aaaatgtctt tatcctatta
     3481 tgttggttac acagatgata cctacaataa ttatagcacc actctttatg atatggtttg
     3541 gatttggaac aatgccaaaa gtattgatgg taatgcttac ttgttttttt cctatattaa
     3601 taagttttgt agatggcatt gagaatattg ataaagatta tttaaattta tttaagacta
     3661 tggactcaaa taaagttaat acattcattc accttaaatt tcctatggca atggataaat
     3721 ttttttctgg acttaaaata tctgcaacgt atgctgtaat ggcagcaact gttgcagaat
     3781 ggctaggtgg aacgaaagga cttggtgttt atatgcttag gtcaaaaagt gcatatgcac
     3841 ttgataaagt atttgcatct actatattag tagttatatt tagtttaatg tttgtaggaa
     3901 ttgtacaggc tgtaaaaaaa gttgttataa gacatagatt aatagattaa gggtatttga
     3961 gggaggcaat tatgaatttt aaaaagttag gaatattagg tttagtaagt gtttttacat
     4021 tggcaacaat aactggatgt tcttcaaata acaataaaga caaaaacaat gataaagata
     4081 cttcttctaa aaaagtaact atggtgcttg attggacacc aaatacaaat catactggat
     4141 tatttgtagc acttgataag ggttactata aagaagaagg tttggatgtt gaaatagtac
     4201 aaccacctga aagtggagca gaaactttag tagcaacagg aaaagctgac tttggtataa
     4261 gctatcaaga acaagttact tatgcaaaga ctagtgaaga tccacttcca ataaaagctg
     4321 tagcaactgt tatacaacat aatacttctg ggtttgcatc accaaaagaa aagaatataa
     4381 caacagctaa agattttgaa ggaaaaactt atggtggttg gggctctcca tcagaagaag
     4441 ctgtttttaa agcagttatg aagaaaaatg gagctgattt taataagtta aagatagtta
     4501 atactggtca agatgatttc tttgcagcta tgaaaactgt agattttgca tggatatttg
     4561 aaggttggga tgcagttaaa gctgatttaa ttggatatga cttaaacttt ataccagtaa
     4621 aagatttaga tgagagattg gattattata caccacttat aatttctaat gaaactgtgc
     4681 ttaaaaataa tccagagctt gctaaaaaat tcttaaaagc tacaacaaaa ggttatgaat
     4741 atgcaataaa aaatccagaa gaatcagcaa aaattttagt aaaacatgca cctgaagtag
     4801 atgaaaaact agctttaaag agtcaagaat atttagcaag caagtacaaa gatgatgcac
     4861 cacgatgggg agaaatgaaa gatagtgtat ggaacaacta tacatcattc ttaaaagaat
     4921 ataaattaat agataaggat atgaaggcaa gtgatgctta cacaaatgaa tttttaccac
     4981 aataaaaaag taaaagtatc tatagaaaat gtatcaaaaa cttataaaga tattgaagtt
     5041 ttaaaagata taagtattga tgtttatgaa ggtgagtttg tttctatatt agggccaagt
     5101 ggatgtggaa aaagtactat ttttaatatt ataactaaac ttacagatta tgatagtggc
     5161 aaagtgaata taaatggtaa gtatagttat atgtatcaaa aagatttact cttgccatat
     5221 aaaactataa tagataatgt gtcactacca ctaattttaa aaaaagaaaa aaagagtaaa
     5281 gctaggtcaa tggtaaaacc ttattttgag gtgtttggat taagcggata tgaagataaa
     5341 tatccttcag aactttctgg aggaatgaga cagagagcta acttcttgag aacttttata
     5401 aattctaatg acataatgct tttagatgag ccttttgggg cacttgattc attaactaaa
     5461 acatccatgc aaaaatggct tttagatgtt aagaaaaaag taaattcaac aatacttctt
     5521 ataactcatg atattgacga agctataatg ctatcaaaca gaatttatgt tatttcaaaa
     5581 aaaccatcaa tagtaaagaa agaatttgtt atagatagta gaaagttaaa tgaagataac
     5641 cttgaaaata tcattaaact taaaaaagaa ataatatctt tattgtaaaa aaaaatattt
     5701 ttatttaata agcattaaac ttagcaatcc aaaatgtgag tatacatttt ggattgtttt
     5761 tagttatatg tcaaaatttt atatattgtg ttaataataa tcatctacta tgatataata
     5821 tttaattaga aaagaaggag gtttgataat gcaaatagat atgaataaat taactcctat
     5881 gatgaaacag tatcttgaag taaaaaatag atataaagac tgtatactgt tttttagact
     5941 tggagatttt tatgaaatgt tttttgaaga tgcattagta gcttcaaaag ctcttgaaat
     6001 agcattgaca ggaaaagctt gtggtttaga agaacgtgct cctatgtgtg gtgttccttt
     6061 ccactcagcc aattcatata tatcaaaatt agttgaaaat ggatataaag tagcaatagg
     6121 tgaacaaatg gaagacccat ctacagcaaa aggaattgtt agaagagagg ttataagggt
     6181 catcactcct ggtacagttt tagatggaaa tttacttgaa aataaaaaga ataattattt
     6241 attatctttg tataaggatg gtactaatat aggtctaact tatgttgata taagtacagg
     6301 agaaactaat gcaacatgtt taaatgaaga taaggtaatt gaagaaattg caaaaatcca
     6361 tccaactgaa ataattataa atgatttaga ctttatagaa aagcttagag atatagctac
     6421 tgtaagtaat atatacataa atgaaagttt tagtgataac tatttggata taaatatatt
     6481 aaaagaatat tttccagatg tttatctaca aaaactaaag tttgatgata aaggtctaat
     6541 aaagtcaagc ttatcaattc ttctgaatta tatatacaat actcaaaaac aaataacatc
     6601 taacattaat aatataaata tatacaattc atcagaatat atggttctag atatgtttac
     6661 tagaactaat ttagaactta ctcaaactat aagagggaat aagaaaaaag gttcattatt
     6721 acatgtactt gacaaaactt ctacagcaat gggaggtaga cttttaagaa aatatgtaga
     6781 agaacctttg ataaataaat ctaaaattga aaatagatta gatgtaatag aagaaataaa
     6841 agatgacttt atattaagag aagacctaaa tgatatactt aaaaatatat acgatataga
     6901 aagaatatgt ggaaagatag catttgagag agtaacacca aaagagttaa ttcacttaaa
     6961 aaattcgata gaaaagttac ctaatttaaa agatactata aatttatcaa atgcaaaaat
     7021 cctaaaagag tatgtttcag aaatggataa attagatgat atatacaatt taatagatga
     7081 agctattcta gaagaaccta cgataaccat aaaagatgga aacataatta aatcagattt
     7141 tagtgatgaa cttaaggaac taagagagat ttctaagaat ggagcctttc ttgtaaagga
     7201 aatagaaaac agggaaagag aaaaaacagg agttaaatca ttaaaaatag gatttaataa
     7261 agtttttggg tattatatag aaataacaaa agccaacttt aaacaggcaa agctagatga
     7321 gacatatata agaaagcaaa cacttagtaa tgcagaaaga tatataactc cagaattaaa
     7381 ggaaatagaa gaaaagatac tacatgcaga ggaaaagatt aaatctttag aatacgagat
     7441 atttgtagag ataagagata caatatataa aaacatagat agaatacaaa aagtggctaa
     7501 gactatagct aatatagatg tttttgtgtc ccttgcaaca gttgcacata taaataacta
     7561 cgtaaaacca gcaataaatg aaaataataa acttgatata agaaatggta gacatcctgt
     7621 tgtcgaaaat atagtaggag aggaaaattt cgttccaaat gatacttatt taaatcgtgg
     7681 agaaaacatt attaacataa taactggtcc taatatgtca ggtaaatcaa catatatgag
     7741 gcaaactgct ataatagctc ttatggcaca tataggctca tttgtaccag cagagtctgc
     7801 tgatattcca atattagata gaatatttac aagagttgga gctagtgacg atttatctca
     7861 aggtcaatct acttttatgg tagagatgaa cgaagtatcc cttatattaa aaaatgcaac
     7921 agagagaagt ttagttatat tagatgaaat aggtcgtgga actagtacct atgatggtat
     7981 aagtttagca tggtcaatag tggaatatat tcaaaaaaat attagatgta agacattatt
     8041 tgcaacacat tatcatgaac ttactgattt agaagaagag tttaaagagg ttaaaaatta
     8101 ttcaatagca gtcaaagaag atggggaagg aataatattt ttaagaaaaa taattcctca
     8161 gggagcagac aagagttatg gaatatatgt tgctaaatta gctaaattac cagacgaagt
     8221 catagaaaga gcaaaatata tacttaaaga cttggaaaaa aatcatgttt acaacagtgt
     8281 tgcaataaat ggagataaag aaaacaataa tataattaat agtaatttgg acgaagaatt
     8341 agttaaggta aatcaaagta attttaagaa taaatatgaa tctctaaaaa tagaacatga
     8401 acttatagta aaagactaca aacatataaa aaaggattat gataaattaa attctaaatt
     8461 taaagcatta aatgatgaag ttgctttaat gaagcaaaat gatgataaag agaaaattaa
     8521 tcaagaagat tctgtaaaag aagtagcctt aactcaaatc tcttttgata gtgtaaatag
     8581 agatatacta tcagaagaaa ttttaaatct ggatatttta aatatgactc cattagatgc
     8641 tataaactct ctttataatc ttcaaagaaa agcaaaagaa ataaaatctt agaggtgtaa
     8701 aaatgaaaaa tataattaac atcctagatg atttgactat aaataagata gcagcaggtg
     8761 aagttgtaga aagaccttct tctgtagtaa aagaacttat tgaaaactcc attgatgcag
     8821 gtgcaaataa aatttccata gatataattg atggaggaaa aagtctaatt aagattacag
     8881 ataatggaat aggaatacct tcaagtgaag ttgaaaaatc atttttgaga catgctacta
     8941 gtaaaattaa aaaaattgat gatttgtatg atttatactc tcttggtttt agaggagaag
     9001 cattggcatc tatttcagct gtatcaaaat tagaaatgac gactaaaact aaagatgaaa
     9061 taataggaac taaaatatat gttgaaggtg gaaaaataat atcgaaagaa ccaataggtt
     9121 ctactaatgg aactaccatc attataaaag atattttctt taatacacca gcaagacaga
     9181 aatttttaaa atctactcat gcagaaacta taaacataag tgatttgata aataaacttg
     9241 caataggtaa tccaaacatt caatttaaat atacaaataa caataaacag atgctaaata
     9301 ctccagggga tggaaaactt gtaaacacta ttagaagtat atatggaaaa gaaataacgg
     9361 aaaatataat agatgtagag tttaagtgca atcactttaa aatgaatgga tatataggta
     9421 ataacaatat ataccgttca aataaaaact tacaacatat ctatataaat aaaagatttg
     9481 taaaaagtaa aataatcata gatgcaataa cagaaagtta taagtctata attccaatag
     9541 gtaaacatgc agtgtgcttc ttaaatatag aagtagaccc atcatgtata gatgtaaata
     9601 tacaccccaa taaacttgaa attaaatttg aaaaagaaca agaagtgtat atagaactta
     9661 gagatttttt aaaggttaaa ttaatacact caaatcttat tggaaaatat gctacttata
     9721 gtgataaaaa aacacaacct agaatcgcta ttaacagtag agaaaaaagt acagattata
     9781 aattaagaaa taatgattta ttagaatcta ctcctaaaaa tagtaatatt actaaaggta
     9841 aggatgaagt cattgaagtt gttactttat caagtgaaaa accaataaat gaatttcaat
     9901 cagtaagtga agtcctaaat gcaagtgtag aagatgatgt aaaaaacatt aattatctaa
     9961 gtgaagatag tgcaaatgat aatatacaag aagagtttca agttgatgga attaaaaatg
    10021 aaggaaatta ttacttagga gatagtatta aggacagtga agaagaatac ttgtgtagtt
    10081 caaaacgcaa gttttcttta tacggatatt ttgtaattgg agttgtattt aatacttata
    10141 taattttgag caaagacgat tctatgtatt tgttggacca acatgctgca catgaaagaa
    10201 tactatatga aagatatatg gagaagttct atagacaaga tataaatatg caaatacttt
    10261 tagacccagt ggttattgaa gtatcaaata tagatatgct tcaaattgaa aataacttgg
    10321 agttatttat gaaatttggt tttgagctag aaatttttgg caataatcat ataatggtaa
    10381 gatgtgttcc tactatattt ggagtacctg aaactgagaa atttatactt caaataattg
    10441 ataatataga agaaattaca agtaattatg atttaaaagg agagagattt gcatctatgg
    10501 catgtaggtc tgctataaaa gcaaatgata aaatatacga tattgaaata aaaagtttat
    10561 tggaacagct agaaaagtgt gagaatccat ttacatgtcc tcatggaaga cctataatgg
    10621 tcgaaatatc taagacagaa atagaaaaaa tgtttaaaag aattatgtaa aatatttgga
    10681 tatttaaaat atatggaaag gaatgttatg aaaaaaatac cacttataat acttacaggt
    10741 ccaactgctg ttggaaaaac agatttgtca ataaaattag caaaagatat gaatgcagag
    10801 ataatttcag ctgattctat gcaaatatat gaatatatgg atattggaag tgcaaaagtt
    10861 acagaggaag agatgcaagg agtgaagcat taccttatag atgaagttaa gcctgactat
    10921 ccattttcag tttcagagtt tcaacaaaga gcaaaaaaat atatacatga aataaatgaa
    10981 aaggaaaaat gtgttttagt aactggaggc acaggacttt atttaaactc tttaatatat
    11041 aatatggatt ttgcacaatc tgatgccaat aatgaattaa gagaagaact tcaaaaacag
    11101 cttgctgaaa agggaataga ttacatgcat aataagttaa aagaacttga tgaagaatct
    11161 gctaatagaa tccataaaaa caatactaaa agggtcataa gagctcttga agtttgtcta
    11221 agtggtaaga aaatgaatga tttttcaagt gatttaaaat ttaatgaaga atatcaacca
    11281 ataataatag ttctaaatag agatagagaa catttgtatc aaagaataaa tatgagagtt
    11341 gatattatga ttaaaaatgg attggttgaa gaggcaaaaa aacttttaag tatgggattt
    11401 aaaaaagata tgatatcaat gcaaggtata ggttataaag agatattaaa gtatctggat
    11461 ggagaatata catacgaaga agctattgaa ataattaaaa gagattctag aaggtatgca
    11521 aaacgtcaga taacatggtt taaaagatat aaaacagcaa aatggtttga tttagaccaa
    11581 tatgaaaata tagacgaatt aaaaaatgaa ataatcttat atattaaaga ttctataaaa
    11641 taa
//
LOCUS       DAEOSP010000125        11594 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.126, whole genome shotgun sequence.
ACCESSION   DAEOSP010000125 DAEOSP010000000
VERSION     DAEOSP010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11594
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.126"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            95..1477
                     /locus_tag="KRH54_002511"
     CDS             95..1477
                     /locus_tag="KRH54_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG8580161.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDKPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMIGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            1489..2838
                     /locus_tag="KRH54_002512"
     CDS             1489..2838
                     /locus_tag="KRH54_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBG8580162.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            2988..3521
                     /locus_tag="KRH54_002513"
     CDS             2988..3521
                     /locus_tag="KRH54_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG8580163.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            3605..4414
                     /locus_tag="KRH54_002514"
     CDS             3605..4414
                     /locus_tag="KRH54_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8580164.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            4825..6870
                     /locus_tag="KRH54_002515"
     CDS             4825..6870
                     /locus_tag="KRH54_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8580165.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            7131..8330
                     /locus_tag="KRH54_002516"
     CDS             7131..8330
                     /locus_tag="KRH54_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBG8580166.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARNLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            8382..9077
                     /locus_tag="KRH54_002517"
     CDS             8382..9077
                     /locus_tag="KRH54_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBG8580167.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            9094..9771
                     /locus_tag="KRH54_002518"
     CDS             9094..9771
                     /locus_tag="KRH54_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580168.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            9882..11483
                     /locus_tag="KRH54_002519"
     CDS             9882..11483
                     /locus_tag="KRH54_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG8580169.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     YDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYPRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
BASE COUNT         4554 a         1160 c         2203 g         3677 t
ORIGIN      
        1 taccttattt taagtaatat agtatataat gtttgttaac aagtaaaaag attgaataaa
       61 agttaattaa aaatttaaag taggaggtaa atttatgctt tcaaaaaata tagcttctaa
      121 ggtattagaa aagtgtctaa ttactggagg agattttgct gagatttttg aagaagacac
      181 tataaataat tctattagtt taatagataa taaagtagaa aatgcaatag gtggaagaaa
      241 ttatggtata ggaataagaa tatttaaagg attgaaaagt atatatgctt atacaagtga
      301 caattcatta aatagtctgt tggatgtagc atacaaagct gctgttgctt taggtaaatt
      361 ggaagatgga aaaagtgtaa tattaaatga cagtattaaa ataaataaca tacataatat
      421 aaaaatttat ccaaattcaa taggaaataa agataaagta agtgttatga aggttgcata
      481 taagagtgca aaagaattta gtaatgatat atctcaagta agtgttagtt atttggataa
      541 agaccaaaaa atattaattg caaacactga aggtatatat gttgaggata gaagaataag
      601 aactagacta gggataagtt caattgcatc taagggtaat gaaaatcaaa ctggatttga
      661 aggtcctgga ggttgtaaag ggtttgaaat atttgaagaa atagacccag aatattatgc
      721 aaaagaatca gctagagtag cacatactat gttgcatgct aaaaattgtc ctgcaggtaa
      781 tatgatggta gcaatagata atggatttgg tggagtaata tttcatgaag cttgtggaca
      841 ttctttggaa gctacatcag ttgcaaaggg gaactctgta tttgcagata aattaggtca
      901 acagattgct tccacaaaga ttacagctat agatgatggt acaataccaa actactgggg
      961 ttctatgaat atagatgatg aaggaaatcc aactcaaaag aatatactga ttgaaaatgg
     1021 aattttgaag tcttatatga tagataaact taatggtaga cgaatgggaa tgaatcctac
     1081 aggaagttct cgtagacaga actacaaatt tgcaccaact tcaaggatga ctaacactta
     1141 tattgctgct ggagaggata aaccagaaga tattataaag tcaataccag atggacttta
     1201 tgctaaaaaa atgggtggtg gttctgtaaa tcctgtaaca ggagagttta attttgcagt
     1261 ttcagaaggt tacttaataa aaaatggaga aatacaagag ccagttagag gtgctagttt
     1321 aattggaaaa ggtagcgata tacttatgaa tatagatatg attgggaata acgtaaaaca
     1381 agctcagggg atgtgtggct catcatcagg aagtatacca actaatgtag gccaacctat
     1441 gataagggta aaagaaatta ctgtaggtgg aagataggag ggataattgt ggaacttaaa
     1501 aattttaaag atatattatt taaaaaggct ctaagtgaag gttttgaaga gtgtgaaata
     1561 tattattata caggagaaaa tttaagtata aatatatatg aaggagaagt tgagaaatac
     1621 aatcttgata aatcttttgg tttatctttt agaggtaagg taaatggaaa gataggttat
     1681 tcttatacag aaattttaga tgataaagct gttgatatgt tgataaaaaa tgtaaaagat
     1741 ggagtaaaca ctattgaaaa tgaagatgta caatttatat atgaaggaga taagcactat
     1801 aatgatgtaa agacttattc taaagagcta gaaaatttag aggcagataa gttgattgat
     1861 ttagctttag aaatggaaag agagactaaa gcttattcag ataaggtggt aaatttatct
     1921 aaatgtacta tatcttattc tgtatcaagt aatggtatat ctaatactaa aggtcttaat
     1981 ttaagcaata aaactaatat gcttataggg tttgtagttc ctatcataga agacaatgga
     2041 caaaaatatg atggaatagg atataatata gcaaattcta ttgaagaaat aaaaccttgt
     2101 gagatagcaa aacttggtgt tgataatgct ttatctaaag taggtggtaa gagtattcca
     2161 tcaggtaaat ataaaactat attgcttaat gaagctatgg tatctttgct tagcacattt
     2221 tctgggatat ttaatgcaga ttcagctcaa aaaggattat ctttattgaa aaatagagaa
     2281 ggagacatga tagcatctcc tatagttaca atagttgatg acccactttt agaaaatggt
     2341 atggcatcta ctccttttga tgatgaagga gtagctacat ttaaaaaaga agttgtttta
     2401 aatggaaagc ttataacttt acttcataac ttaaaaacgg ctaataaggc aggtgtaaag
     2461 actacaggaa atggaataaa atcttcatat tcatcaccta taagcatata tccaactaat
     2521 ttttatatag agaaaggaga taaatcttta gatgaaatta taaaggatat agatgaagga
     2581 ttgatggtta ctagctttgc aggacttcat tcaggtgcaa attcagtaac aggagacttt
     2641 tctttagctg ccaaaggctt ttatatcaaa gaaggaaaaa aagtatttcc agtggagcaa
     2701 attacagtag caggaaacta ttttgacctg ttgaaagata ttgaagctat aggagaagat
     2761 ttagaatttc caatgagtag tataggttca ccttcagttg ttattaaaga gctatcagta
     2821 gctggaaaag atgaataatt aaaaagtaat tttgtggata aaaggtcatg ttaattttaa
     2881 ttaatatggc tttttattat ttaagtagat tttattatct aagttatatg aaaaagttgt
     2941 gatgcataca attaatatca tacattaaat ttaaaggagg agatattatg agtgatgcac
     3001 attatgacat gcacaagcga aatggatatt caagtgatga gccatatcca gagataaaag
     3061 ttttaggtcc aaataagtac tatgcagaat tattgatgga tgactatgcc ggagtatcaa
     3121 gtgaatttac gagtgtaaat caatatctat accataattt tgatttagat gaaacgcata
     3181 gagagttaag tgaaatgtgg ataaatatat ctataacaga aatgttacat atggagatat
     3241 tagcaaagac tataagatta ttgggtggaa atcctgtata tagaggttct acaagttctt
     3301 gtggtgcata ttggaatggt ggttttgtat gttatggaaa ttctatttgt aacagattaa
     3361 aactagattt gcatttggag catgtggcta ttaataatta ttataaagac ataagtctta
     3421 ttgaagaccc ttatataaaa gctatattaa atagaataat cctagatgaa aaacttcatg
     3481 taagtttatt tgaaaaggca atagagaagt attgtaaata aatttgaaaa tttagttatt
     3541 catcatttta ttcatatata taaaataacc atcatacatt ttcttgaagg gagttgctgt
     3601 tgatatgtat tatgtatatg tgaatgatat aaacatagca gtatatgatt tgaatccgag
     3661 tgctaaaaaa actgtacttt ttatacatgg ttggccctta ggacataaga tatttgaata
     3721 tcaaacaaat atattgccaa aactcggata tagaacagtt tcaattgatt taagaggttt
     3781 tggtaaatct gatgcaacat caggaggata tacttatagc caattagcag acgatattta
     3841 taaggtggta catgctattg gattaaaaga ctttaccctt gttggttttt caatgggagg
     3901 agcaatcgta cttaggtata tgagtttatt taatggatat ggagtgtcaa aattggtatt
     3961 agctgcagct gctgcaccat catttgtaca aagacctcca gaatttccat atggtatgac
     4021 acgtgaagat gtaaataaat tgatagcaca agcatgtact gatagaccag aaatggtaac
     4081 cgattttggc gaaaaagtat ttgcatcaaa tccaccagaa agctttagaa gatggtttaa
     4141 tgatattggt tttagtgcat caggtattgg cactattgga actgctgtat ctttaagaga
     4201 tgaagaatta tttaatgatt taaaatgtgt aagagtgcct actggtatat ttcatgggaa
     4261 attagatgaa atatgtccgt atgaatttgc agtttttatg aatgaaaaaa tagaagattc
     4321 aattttatat acttttgagt atagtggtca tgctatattc tatgatgagc ttaaactatt
     4381 taatcaggaa tttttacagt ttttagagga atagatatat tttattatca cttagaaaaa
     4441 gagtaatatt atgtgaaaag atttaagcta aattttatga tgttttttac actaaacaaa
     4501 tagggtttta gaacttgtaa ctaagttctg agaccctatt attatatatt cctaaaattt
     4561 gttataattt attccttgtc attcttaaaa atgtattaat atattaacga atttatatta
     4621 aatttttgtt tatattccaa taatgtcaaa ctaaccgttt tagaaattaa attatatgtg
     4681 aaaaaagata gcttacatta ttttattatg gaaaataata gtaaatggta caaaatttga
     4741 tttttaagat ttaaaacttt tttaaaaagt gctacaatag accaaattat tactttctat
     4801 actcgttaaa caggaggtgg gactatgaaa aaacataata ttttatttgt atcaactgat
     4861 gacaaaataa atatagatat atcaaaacaa ttggaaaata tatttggaga attttgtaat
     4921 atagataact tggtatatgt aaataggata aatattgaac tgtcaagcta tgagcttgta
     4981 gtatgttcag acaatgatat aaaggaatat atacataata atattgataa aaatatacct
     5041 attgtaatag tacatcgtac tataaatatt gaaaatataa atcagataat aagtatagaa
     5101 aatgattctg atgttatggt tatagatgct tataaagagt cagcagatga aactgccaag
     5161 ataattagaa aactaggatt aattcatatt aatctaatac catattatcc aggttgtgat
     5221 aagtctaaat gtgagattgg aattattaca ggaagcagaa acagcatacc acaaaacata
     5281 aagcaaataa ttgacatagg tgataaggta attgatataa atacagttat agaaatcttt
     5341 acaaaactaa atatatctat agataaatta catattatta aggaaaaata caatgaagat
     5401 acagtaagtg gatataggta ttatacaact atgaataaaa ccatgaagag ttttttggag
     5461 attatagatg aaggtattgc ttctgttgat aaattaggaa aatttatata ttgcaacaaa
     5521 gtattttcta atctagttgg aatagaccaa aatgaaatta tatctaataa ctttatggac
     5581 ttatttagtg ataaggttgt caaaaaaata ttttttcaag aagatgaagt taatgatgaa
     5641 gtagtaaatt taaataacaa aaaactgata attaataaag taaatgttta tgaaaataat
     5701 gaacgaataa agagtattat aagcattaaa aatataagtg caattcaagt gcttgaggac
     5761 aagattcaaa ataaacttca gtcaaaaggt tttgtatcaa agtatacatt tgagagtgtt
     5821 gtaggtgaga gtaaaataat aaaagaaaaa attaatattg caagaaaaat agcaactaca
     5881 gacttttctg tgttgatact tggagaaaat ggaacaggaa aagaaatatt tgcccaggca
     5941 atacataatg aatctctaag aaaaaacaag ccatttgttg cagttaatct atcttctttg
     6001 tcagatacac ttattgaaag tgaacttttt ggctatgatg aaggaagttt tacaggagca
     6061 ataaaaggtg ggaaaatggg tatatttgaa agagcacaca caggaacaat ttttttagat
     6121 gagataggtg atatatcttt agatgtacag cagagattgc taagagtatt acaggaaaaa
     6181 gaagttatga gaatgggcgg aagtaaaatt ataccaatag atgttagaat tatagctgca
     6241 acaaataaag atttaaagaa aaaaattaaa gaaggttcct ttagagaaga tttgtattat
     6301 aggattaatg tattacatat agagatacct agacttagag aacgtaagga agatatacct
     6361 cttataagta agtatttttt agatgaaata aatagcaaca aatgttttac agaggaatca
     6421 atgcaagctt taacattgta tgaatggcca ggaaatgtaa gagagcttaa aaatttaatt
     6481 tattatattg atactatagt tgaggaagat agagtagatt acgagcattt gccagaacaa
     6541 tttagatttg aaaaaaacaa tactttagtt aatgaaaatt tcgattcaat tatcttagat
     6601 ttcaaacaat ctaatttctt tgaggaaagt atatgtatat tgaccagtat agaaacttgg
     6661 aataataaaa atatattatt aggaagaaat aaactgcaag agattttaaa agaaaaagat
     6721 atagttttaa gtgtagacca aattagaaaa agaatagata agttaaagag ccatggttta
     6781 cttttatctg gagtaaaaaa acaaggtagt tttataactg atgaaggaaa gaactttata
     6841 tcatatgtaa aaattaaggg agtgatatag gggactaaaa aggcaaaaca ccctaaaaaa
     6901 atccctataa tggatagaaa taaataaaat tatctttaag attaagataa ttcaaaaata
     6961 ttgaataaat aatctaagaa gattattttt aataaaacag tagtaaatat caaccttact
     7021 actgttttta ttttttgcaa gatttattat acaattaaaa tttatatttt ggcacatcaa
     7081 ttgctacata ggaaaaatat aaaaaattgt aaaacttgga ggatgttgtt atgaagtcag
     7141 cttacaaagg atatgaagcc tatagttatt tagaaaaagg aaaagatatt tcatctattg
     7201 agatgtgtaa aggagataaa agagttaaag aataccttat tgaattagat gatattcaag
     7261 aagctaaggt taaaagtata gtggatgaaa aaatatttat atcattacat gaacatccag
     7321 tattatttcc agaacaccta gaaagagata tatttgaata taatagggaa ggtaaacaaa
     7381 gatgtgctta tgaagcatta tcaagaggat attatgatgc tatatttgat aatttaatgg
     7441 atggtgtatg tacgataaca tcaaattcag ggtggaaatg ggatgatata ttaatagatt
     7501 taggcatgag attatgtgat ttagctcatc aagattttgt cataaagtgt gaaaaagtag
     7561 aagatatata cagagctaaa agtgaaggta aagtagcatt gataccaaca ttagaaggtg
     7621 cagcaccaat agaaaatgaa cttgatagaa tagatatact gtatggattt ggtataaggt
     7681 taatgggtat aacatacagc gaatccaatg cattaggttc aggactaaaa gaagagaaag
     7741 atggaggtct tacaaatttt gggaaaaaag ctgttgaaag aatgaataaa attggaatgg
     7801 caatagattg ctctcacgtt ggagttcaaa ctacacttga tgtaatagaa tatagcaagg
     7861 acccaataat attaagccat gttggggcaa gaaatttatg gaacacaaag agactaactc
     7921 cagatgaagt attaatagca tgtgctaaaa aaggaggggt tataggtgta gaagcagcac
     7981 ctcatactac aataacagag aagaataaag aacattcaat agagtcattt atggaacatt
     8041 ttgagtatat taaaaattta gtaggaatag accatgtagc ttttggacca gatacagtat
     8101 atggcgacca tgtaaaatta cactcagtac tagctgctaa atttgatata aaaaaaataa
     8161 caggaacaaa agaacatccc aaagtagaat atgtaaaagg tcttgagaac ccaacagaaa
     8221 cttcaaaaaa cattgttaga tatttagtaa agcatggata tagtgatgag gaaatagaga
     8281 aggtactagg tggaaatata cttagagtac taaaacaggt ttggaaatag atatattaga
     8341 aatattaata aaatttgagg atttataggg aggttttata aatggattat ttaaaattag
     8401 ctaatcatcc acttttgtgg atagcgagta cagtagcagt atcaatagta gtggtacaat
     8461 cccttatatt tgctataaaa tcttgcaaag ttggtaaaac aatgggaata acagataaac
     8521 aaataaagtc agctgttaag agtagtgcta tttcagctat tgggccttca atgactatat
     8581 ttgctggaat ggtatcgctt atagtaacta tgggtgggcc tatagcatgg atgagattat
     8641 catttatagg ttctgtaatt tttgaatcaa tgtcagccgg atttggaaca gatgcattag
     8701 gtataacttt aggttctcct gaaatgacaa aacttgcatt tacaaatgca gtttggacta
     8761 tgatattagg ttctttagga tggataatat tcacattact atttggacat aaactagata
     8821 agataaccaa tgttatatct agtggcaaga aatcatttat accaataata tctctaggag
     8881 caatgttggg ttcatttgca tatctaaatg ctgatagggt gttgcgtttt gataatggaa
     8941 caattgcatg tataagtgga atggctataa tggtaatatt gtgtatgctt gaaaagaaga
     9001 aaaatataaa atggttaaga gagtgggggc ttacaatatc aatgtttagc ggaatgataa
     9061 tagcttcagt aatataaggg gggagttagt tttatgagta aagtaaaatc tatatacaat
     9121 gaagaatatt taccttttat gataaggtat ggaagattaa cactatcttt gggtattata
     9181 gcagcattag ttcctggaat aattttgagc tttggatttg gtattatgcc tccaatatca
     9241 gcattattag caagtacaat ggcaattgtc agtatgagtg ctccaaatta cataatagaa
     9301 ccagtttctt attcaccaat cttaggtata ccaggaactt atatgagttt tctttctggt
     9361 aatatatcta atatgaggct accatgttca atagcagctc aaaaggcagc agaagttgaa
     9421 tcaggtacag aagaaggctc gataatatct acaataggaa tagctgtatc tattttagtg
     9481 aatataagta tactaactat aggggtaatt ttaggaggaa gcgtactatc taaaattcca
     9541 gctgaagtag tagaaaaatt aaacttaatt ttaccagctc tatttgggtc agtttttgga
     9601 caagtatttt tacaagataa aaaattaggt ctagtagcaa ttgtaatatc tgtattgact
     9661 attatattat caaagcaagg tataatacca cagtctctag tagttcttat atgtgtattt
     9721 ggtactatat taattgcaag agctatgtat aaagataagc ttagtgacta attacaatga
     9781 aataatagtg aattttcaaa tatgaaattt aataatttga ttttcaaata taaaaattac
     9841 attaagatta tttaaaaatt ggtttttaag gagggaatat tttgatatat gatttaatct
     9901 taaaaaatgg atttataata gatggtacag gtaatcctgg tttttatgga gatatagcaa
     9961 ttaaagacaa cttaatagca aaaatagatt ctaagataaa tagcaataca aataaagaga
    10021 ttgattgttg tggaaaagtt ataacacctg gatttattga ccctcatgtt catgaagaaa
    10081 tagtagcaat actagatggt aaatttgaaa agtttctaaa acaaggagtt acaacaacta
    10141 taaatggtaa ttgtggacat tctataacac catattcttc tgaaaatgtg tatgaatata
    10201 tgtataaaaa tggtctatta cttgaagaag aaaaaaagta tcttatagat aaaaataaat
    10261 gttggaataa ttttacagaa tattgtgacc ttatatctaa aagtggtatt tctgtcaata
    10321 tgggattctt gttggggcat gggactataa gatggagtgt tatgggaggt tctaaggata
    10381 gacctcctac agaaaaggaa aaaaatgaaa ttacagatat aataaatgat ggaatgaaaa
    10441 gtggtgcttt tggaatatct actggattgg catatatacc aagtaagtat gctgatattg
    10501 atgaacttgt tgatattgca aggcagatta aagagtatga tggtatatat acatctcata
    10561 ttagagatta cataggaaga tataatgcag taaaagaagc catagaggtt gggcaaaaaa
    10621 gtggtgctag ggttcaagta tctcacttaa gtccagttga aatagaagca tttgatgaga
    10681 tattaaaggc taggtataat ggtgttgaaa taatggttga cacagtacca agaagttctg
    10741 ggcattgtat gaagaaaaaa agagtaattc aatttattat ggcaatatca tctagtctat
    10801 ttgaattagg tatagatggg gtaatggatg ctcttagaaa tgaagaggga agaacactta
    10861 tattaaaaga agcatttata cttggagata gggggagtat aatacttcta aacactaaag
    10921 atattaatat ggaaaagaaa tcaatacgtg aaatagctac acaaaaagga atagatgaag
    10981 acaagttatt actcgattta cttttagatg gagacgaaga acttatcttt tgtttaggag
    11041 gaatgtatag agctgatttc ccagataaac tacatgatga taaaataatt gataatccat
    11101 ttgttatggt tggttcagat tgtttgttta gtgtgggtgg ggatatgtca tggtttgaac
    11161 tacaaagaag aggagctttt ccaattttct tcaatatgta cagaaagtct ggtgttagat
    11221 tggaagagat tgtaagaaga gttacttcat taccagctag gcaatttaaa ataaaaaata
    11281 gaggtattct taaagaaggg ttgatagctg atatagcagt tattgacatg gataactata
    11341 gttatcctag gtcagaagat attgattttt caaagccaca attactagcc aatggtgttg
    11401 aatatgtaat tgtaaatgga aaaattgcat tagaaaatgg agagattact gagaataaat
    11461 gtggtgaagt attgaaaaga taaggttcta aaaataatag gatttagaaa gtaaataaat
    11521 atctaagtaa gtaaagacga agtttgagat ttatttaaat aaaaattatg cttcgttttt
    11581 atttatagta ttaa
//
LOCUS       DAEOSP010000126        11458 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.128, whole genome shotgun sequence.
ACCESSION   DAEOSP010000126 DAEOSP010000000
VERSION     DAEOSP010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.128"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(69..707)
                     /locus_tag="KRH54_002520"
     CDS             complement(69..707)
                     /locus_tag="KRH54_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG8580170.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
     gene            complement(707..1570)
                     /locus_tag="KRH54_002521"
     CDS             complement(707..1570)
                     /locus_tag="KRH54_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580171.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            complement(1567..1941)
                     /locus_tag="KRH54_002522"
     CDS             complement(1567..1941)
                     /locus_tag="KRH54_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8580172.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            2308..2712
                     /locus_tag="KRH54_002523"
     CDS             2308..2712
                     /locus_tag="KRH54_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBG8580173.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            2729..3325
                     /locus_tag="KRH54_002524"
     CDS             2729..3325
                     /locus_tag="KRH54_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580174.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            3362..5956
                     /gene="clpB"
                     /locus_tag="KRH54_002525"
     CDS             3362..5956
                     /gene="clpB"
                     /locus_tag="KRH54_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBG8580175.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(6152..7579)
                     /locus_tag="KRH54_002526"
     CDS             complement(6152..7579)
                     /locus_tag="KRH54_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8580176.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            7720..8616
                     /locus_tag="KRH54_002527"
     CDS             7720..8616
                     /locus_tag="KRH54_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG8580177.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRIDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            9281..9421
                     /locus_tag="KRH54_002528"
     CDS             9281..9421
                     /locus_tag="KRH54_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580178.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(9418..9789)
                     /locus_tag="KRH54_002529"
     CDS             complement(9418..9789)
                     /locus_tag="KRH54_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8580179.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            10338..10595
                     /locus_tag="KRH54_002530"
     CDS             10338..10595
                     /locus_tag="KRH54_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580180.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(10705..11385)
                     /locus_tag="KRH54_002531"
     CDS             complement(10705..11385)
                     /locus_tag="KRH54_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG8580181.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMDTLGAVLGYLIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISEKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT         4631 a         1313 c         1597 g         3917 t
ORIGIN      
        1 atactaccaa aataaactcg atagtatttt atttaaatac ttaaatacag aaacatatat
       61 agtatatttt atacttcttt tttactatag atttttgatg atataaaata tgaaatcaca
      121 aatagtaaaa tagcaacaaa tggaagagaa tatactagtg tattaagtat aatttctata
      181 gaaataggtg aactaatatt ttctaaaata cttttaaaaa ctaataaagc tagtgttgga
      241 actaaaaagc ataaaatcat taataaacga gctctttctg taccaaattt ataaataagt
      301 ggtagaataa ggctaccaaa acatactgaa attgataaga ttagtgcatt aataagtagt
      361 gtttcattta atataagtgt atttttaaaa taaccaatag ttacactaac aataagtgaa
      421 acaagaacac ctataaagct aaatattaac atagttaaat atttacttaa tacaatatca
      481 ttgcgattaa taggcattgt aagtacataa ctatcccatt tattcaaatc atcatatgaa
      541 aatgttgaaa caatcattgt agtacataaa actataataa cacctataaa gttattaaca
      601 tttccatctc ctaaagaaga cataaagcca aacataatga tgaataaaag tataaacttt
      661 atatttcctt tcaaattaag taaatctttt aaaattagac ctttcattat ttatcacctc
      721 tcacataaaa taacataatt tcttctatag acgtattatc aacaatacag tttggatgtc
      781 ttgagataaa tctatgctta tctttaagaa gtacttcata tccaaattgg cttttacgat
      841 aatatgaata atcctctctt gaaaggttat caaaatcact aggtttacat tttaaaactc
      901 ccatagtatc aattaattca tccttatttt cactaaatac aatttttcct ttatgtataa
      961 atgtaatata gtctgctatc ttatctaaat cacttgtaat atgtgaagat aagataatag
     1021 agtgttcttc atcttgtata aaatctaaaa atatatctag tatttcttca cgaacgatag
     1081 ggtcaagtga actagttgcc tcatctagta ttaataattt tggtttatga gataaggcta
     1141 cagcaatact aagtttcatc ttattcccct ttgaaaactc cttaattaat ttattgtctg
     1201 gtactctcaa tttttttaag tagtctttaa ataatgtgtc attccaattt ttatagattt
     1261 tactcatgat ttttgaaaca tggtcagtgt ttaaattttc atgaaagttg cttccctcaa
     1321 atacaacccc aatttgctct ttaatttctt tttcattttt tttactatca agaccaaaga
     1381 tttctatatt tccactatca taagatatta aatttaaaat tgcttttaat gtagttgttt
     1441 ttcctgcacc attttcacct acaaatccca ttacactccc ttttggtact gaaaaagata
     1501 tatcttgtag gctaaaatct ttataagttt tatttaaatt ttttatagtt attgcatcca
     1561 tacttatcat tctcctttat ataataaaaa taaaatatct tttaattcgt catatgtaat
     1621 atcactactt tttgctgtat caactgcttt ttgtaggtat tcttcaatta aacgaaattg
     1681 ttcttctttg acaaaatcca tgtttttact agatacaaaa ctgcctttac cagtaactgt
     1741 ctcaataaat ccgtctcgtt ctaaatcgct atatgctcgt tttgtagtaa ttacacttat
     1801 tctaagttct tttgccaata gtctcatact tggaagagct gagccttctg gtaactctcc
     1861 acttataatc atacttttaa tttgtgtagt aatctgttca tatattggct tcccactcga
     1921 attactaata actatattca ataagacacc tccttaaatt gtatatatag tatatacaca
     1981 ttatataaat atatatataa tatgtcaact gattttagaa aaaactaatt gccaatatta
     2041 tacatttaat aaatgtaatg tattttatga aagtctgcca tattaaatag atataaattt
     2101 ttttaaaaaa tttataaata ttgttcaaaa atcctgccaa taaattggaa atactaaatt
     2161 aaaaaaataa taaagtttag cttatagcta aataaaagtt tacttataag gtaaactttt
     2221 tgcattttta gttttgacat acatattttt ttgtaatata tttatattat caattagaga
     2281 taaggagagt gagaagagat atttaatttg gataagacat taaaaaagga tttaaatcag
     2341 gaatatacag agagcaaaag tttagttatg aataaaagta agtatatgaa tgtttgtttt
     2401 aagaattata ttaaagtagt agaaggaatg tatactaaga gggatttaat tccattaaaa
     2461 ttaataaact ctatggataa aaataataag attagaaaaa cattatatga attatcttgt
     2521 gaatttgaat atccaaaaac ttctttatct tcattgttta cggagctgaa aaaaatggat
     2581 tttttacaaa gagtgaaaaa tggtgagtat atgataaatc cacatatagc atataaaggt
     2641 agtagagctg ataaagaaag tttattagca gaatataatc agataaaaag acctaataag
     2701 gttagtaaat aaggaagaag gtttttatat gagtttaaaa cttgatagaa atgttctaca
     2761 gtggtttgac tatgtatttg agaatgaaaa aactagtttg agacactata attttaattg
     2821 tacactaaag gagattagtt ctaccagttt gaacaaagta gcttttattt tagaaaaaaa
     2881 taactctaag tactggaagc tttattttga aataccagct gaagttactt taaaacttaa
     2941 gcaaaacatt catcctttat ttagggaata tatatacgaa caaatatcac tgtataacaa
     3001 taatcaaatt tacaattttg taaattcaaa catattaaaa gtgtttaata atattgctat
     3061 ttatcaatat aatattttag aaaatctata cactattgat tttaaaaaga gttttataga
     3121 taaatgtcag tatttattaa taggtgaaaa aagacttata gatgaagact tgtatttaat
     3181 agcaaaatca aaagaagtat tcgatttttt taattcagat ggaacattta atttaacatt
     3241 gagtttcgat atacaaaaaa acgagaattt acttgatagt cttttagagc ttagaaaatc
     3301 aataattata aatgagagaa tttaatttat ttggatataa tattaaaatg gagggattta
     3361 tatggacgta gaaaaaatga ctttaagagt tcaaaaaagt ttaaatgagg cttataacga
     3421 agcagtaaaa aatcataacc aacaagtaga tgtaattcat cttttctcag cacttattaa
     3481 tcaagaggat ggattgatac caaatataat cgaaaaaatg aatatatcaa ttgattcttt
     3541 gagaaatact gtaaactttg aaattgataa gcttccaaaa gtatatggag aaggtgctga
     3601 ctcacaagga gtatcagcta ctagaaaaat aaatgaggtg cttataaagg cagagagtat
     3661 ttctaaagaa tttaaagatt catatataag tgtagagcat gttatgcttg caatgatgga
     3721 aacagaatca aaatcagctg ttggtaaaat attaaaacaa tataatataa ataagaatga
     3781 ctttttaaat attttatcac aagtgagagg tagtcaaaga gtagaaacac aagacccaga
     3841 aggcacatat gatgcacttg ctagatatgg aacaaactta gtagatttag ccaagaaaaa
     3901 taaattagac cctgtaattg ggagagatga agaaataaga aggattataa gaattttatc
     3961 aagaagaaca aaaaataatc ctgtattgat tggtgagcca ggtgtaggta aaactgcaat
     4021 tgtagaaggg ttagcagaga gaatagtaag aggagacgtc ccagaaggtt taaaagataa
     4081 gattgtattc tcacttgata tgggagcttt aattgctggt gcaaaatata gaggtgaatt
     4141 tgaagaaaga ttaaaggcag ttttaaaaga agttcaaagt tctgatggaa aaattatact
     4201 gtttatagat gagattcata ctattgttgg agctggtaaa acagaaggtt caatggatgc
     4261 aggtaatctt attaaaccta tgcttgcaag aggtgaattg aactgtattg gtgcaactac
     4321 ttttgatgaa tatagaaagt atatagaaaa agataaggct cttgaaagac gttttcagcc
     4381 tgtaattgca gaagaaccta ctgttgaaga tacaatatct atacttagag gtttaaaaga
     4441 aagatttgag atacatcatg gagtaagaat acatgataat gctattgtag cagcagcaaa
     4501 actttctcat agatatatac aagatagatt tttaccagat aaagcaattg atttaattga
     4561 tgaagctgga gctatgattc gtagtgaaat agattcattg ccaacagaat tagatgttgt
     4621 tagaagaaag ttatttacat tagaaactga aagagaagca ttacttaaag aaaatgatga
     4681 taaaagtaaa tcaagattgg atgatattca aaaagaaata gcagagttaa aatctaaaaa
     4741 tgatgaaatg acagcaaaat atgaaaaaga aaagagtcaa atattagata ttaaaaactt
     4801 aaaggctcaa ttagacgaag ctaaaggtaa agctgaaaag tatgaaagag aatatgattt
     4861 caacaaagca gcagaggtta aatatggtga aatacctaag ttagaagaac aaataaagca
     4921 gtacgaagaa aatatgaatg atggttctga aaattctctt ttaaaagaag aagttactga
     4981 agaagaaata tctagcattg tatcaaaatg gacaggtata ccagtaacta aactagttga
     5041 aggagaaaga gaaaaattac ttaaactaga agatgaactt cataaaagag ttattggaca
     5101 agatgaagca gttacagcag tttcaaatgc agtaatacgt gcaagagcag gattaaaaga
     5161 tgaaagaaaa ccaattggtt catttatttt cttaggccct acaggtgttg gtaaaactga
     5221 acttgcaaaa actcttgcta gaaatttatt cgatagtgaa gataatataa taagaataga
     5281 tatgtctgaa tacatggaaa aacatgctgt atcaagacta gttggacctc ctccagggta
     5341 cgtgggatat gaagagggtg gtcaacttac agaagcagtt agacgtgctc cttattctgt
     5401 aatactattt gatgagatag aaaaagcaca tgaggatgta tttaacatgt tcttacagat
     5461 attagatgat ggtagactta ctgataataa aggtaagaca gtagacttta aaaatactct
     5521 tataataatg acttctaata taggaagtaa ttatctatta gaagcaggag gaaatataac
     5581 agagactact aacaatttag taatgaatga aatgaagcat agatttaaac ctgagttttt
     5641 aaacagagta gatgatatta taatgtttaa gcctttagac caagaaaata tcaagaaaat
     5701 catagatata tttatgaaag atttaaaaaa tagattaaaa gaaaaagata ttactattga
     5761 agttacaaat tcagcaaaag atgttatggt gagagaagga tatgaccctg tatatggagc
     5821 aagacctctt aagagatata taggaaatac attagaaact ataattgcaa agaaacttat
     5881 tgctggagat gtgtataatg gatgtactat agttatagat ggaaaagatg aaaatattga
     5941 agttttagtt aaataataaa agaatcagtt gagcataata tactaaatat tttgctcagc
     6001 tgatttttat ttataaacta taggaaaaga aatattttat caaattaata aaacattttt
     6061 aagtcaatat gtccaaatta aatttaactc aaaggaagta ttttagtaaa tttcttataa
     6121 tataatcata atttgaatat aattataaaa actattgttc atttttatta tatttaaata
     6181 aataaaagcc aattaatatt gcacctatta agcaaacaag tgtaacaata atataaacat
     6241 ttctcattcc aaatacaaat attttatcat tattaatcac ataatcattg accctatatc
     6301 cagctttaat actcataaaa ttatataaaa gtgttgtaga aatagtaata cctataactt
     6361 gtcctaaatt tcttacaagg gaatttatgc ttcctacaac tcctagctta cttctagaac
     6421 agcttgacat tatcaaagca ttgttagcag gttgaaatat agcttgtcct attgatatta
     6481 tcgatatata aattacaaat aaaataaggg cagaactttc tttcaatctt gacattagaa
     6541 agaatccaaa actcataacg agaagaccaa ttaaaataat tttttctgac ttaattttat
     6601 ctgatatatt cccaaatata ggtgaaaaaa tggctaaaat caaaggtgat agtatcataa
     6661 ataaacctgc ttgaataggg ggaagtttca tagtactttg taaataaaat ggaattaaaa
     6721 tacttgatga tgcaatacaa atgaatgatg taagagcaca aattaaattc aatgaaaatt
     6781 taaaatttct aaaaatttca aggtcaagaa gcggttgagg atgttttttt tctaatatca
     6841 aaaataatat aataaaaata attgataata atagtgcaat taatatatat ggatttagta
     6901 atcctgtttg ttgagcacta attagagcac taaaaaataa tgttgttcct aaaaattgaa
     6961 gtatagcacc agttttatcc atattttctg aagatttttt actattagga agaaattttg
     7021 tatttaaaaa gattacaata agaccgattg gaatatttat aaaaaatata acattccaat
     7081 caaatattga caaaataaat cctcccacag atggtccaac catatttcca atagcaactg
     7141 cagtaactag aatccctagg gcttttcctc tgctttcttt tggaaatagt tctgtgataa
     7201 ttccatgatt gtttgccatg taggcagaag cgcctattcc ttgaataaag cggcaaacta
     7261 taagtaaagt taaagaatta cttaatccac atagtaatga agctgatgta aagagaatag
     7321 tacctacttg aaatactctt gatttaccaa taatatctcc aagtcttcca aaaaataata
     7381 gtgttgaaca aataataatt gagtaacttg ctattaccca ttcaatagat gctagagata
     7441 catttagttt gcttgcaaga actgggagag caacatttat aatactagaa tctaatgttg
     7501 ccataaaagt catggcaatg actacaaata aaactgtgat tttttttcta ttatcatctt
     7561 ttttaaaaga gtttgtcatt tttattacct ccttattata atgactgcat tatgttaatg
     7621 tttacaatga tagttacatt ttatataata gcgatatgaa ataatgaata caataactat
     7681 attgcttata attagcacta ttttgctgag gggggaataa tgaaggataa aaatacatat
     7741 tttaacagga aaggtcaaga aaactttata gatatatttt taaaggatga cattaaaaaa
     7801 ggagcaaact ttaaaagaca ttggcatgag catctacaaa tatattattt tattgatggt
     7861 atagcatatc ttgaatgtgg attggataat tttaaggtag tgcctggaaa tattgtaata
     7921 gcaaacagta atgaattaca ttatttagaa agcttatcag ataatttaaa attctatagt
     7981 attcgtattg aacccacatt cctttttagt aatcaagttg atttgttaca aaccaagtac
     8041 ttagcaccat tggctttaaa tcatattgta tttaataatt taattcaaaa tgattctctg
     8101 attttaaaat gtattacaac tatacttgat gaatatcaca ataaagaagt tggatatgag
     8161 ttatctataa aagctaatat ctatcaatta atagtactct tattaagaag tcatattgat
     8221 aaaatatatt ctgaagatga atttataaaa agaaagtatt ctttagaaaa atttgaatgt
     8281 atctttgatt tgatagaaga aaaatatatg gataaaatta cattaaaaga acttgctagt
     8341 cgtgtgaact tgagtgcaca tcacttttgt agaacattta agcaaataac aggtaagact
     8401 acaatcgatt ttataaatgg aatcaggatt gataaagcta tatattattt aaaggaaaca
     8461 gatttaaata tgacagaaat tgctatgaga tgtggctttg atagcataaa ttattttagt
     8521 cgcttattta aaaggaatta taatatatct ccaactaagt atagaaatga acatagagaa
     8581 gttagtaatg ttattttaaa agaagatatg aaataatcca taaatatact ttatagaaaa
     8641 tatatatttt taaatctttg agtttttctt aataaatatt aataaagtat agtacataaa
     8701 tgagtaagac tacttgtata gttaaatgtg aaaatgagta tgaaagaatg cagtgtctat
     8761 gaaaagtata ttttaatatg aaactaaaac tggaaaaata agaatagcag aaaaaatgat
     8821 tatattataa atcagttttt ttgagacatc tggcttagat tctgaaagat atttaatcaa
     8881 agaaataaaa ctattaaata agcaagtaaa cagctaaaat agtatgattt ttatagaaaa
     8941 tttaaatcca tcctaaagaa ttaaaagaaa gtcaaataaa tataaaaaaa ttatatattt
     9001 ataaaaaatt ttataataaa aatcatcctg aaaaatatat aaaaatagaa aaaacataca
     9061 agattctaca ataatagatt tttttgttat tattacgagt tttttattgc aactgtagaa
     9121 taaaagtatt attatataat taagataatt atctagggaa attttggata atcaaaatat
     9181 attttataaa atcaaaatac aattacataa actttattaa aaatttgttg ggaatttctg
     9241 actaacatta agaacgtaag tttgttgggg gatggtatat gtggaaaagt caaacactac
     9301 tgttacaaaa aaagaaaaaa ttgaagatat aggaatgaaa ttagaacatt tattgagtga
     9361 agatattact ttttatgaat tattacttga gattttgtat aaagaaaaag aagagaatta
     9421 attctcttct ttaaggaatc tttttaaata ggcattatca atcatttttt ctatagcttc
     9481 tctatcttct ttttcaagag aatatatttt ttctaagagc ttttttgttt tttcacttac
     9541 tgataagttc tctatgatta atttatcttc tttgcttatt tttgtattat catttacgcc
     9601 taataaataa tctgttgatg tttctaatgc ttttgctaat ctaactatta tttcactttt
     9661 aggttctctt aaattatttt cataacgtga caagctagcc tcagttatac cgactttagt
     9721 agcaagttct ttttgattta tatctaattc ttttcgtaga tttgctattc tatctcctaa
     9781 actttccata tgtacatcac tccttaccga aattataagt tattttgata aaaagtaaaa
     9841 taaaattacc aaaaatataa aaaaaaaagg ttaaaataat aaaatatatt gaaaattacc
     9901 atttgtatat gtataatatg aattatacaa atggtaattt gatgagaggt ggtatgttga
     9961 aataagaaac tgaattaatt aataaataca tagaaagttt tgaaaacctt taattgagat
    10021 ataaacaata tgtacaaaat tttttgcaag taaaattacc aaatgtataa aataaatcag
    10081 tagcatagat aattttatgc tttaatacat tagattattt aatttagtta taatggtcaa
    10141 aaatgcaatc tacaaataaa acaaagtatt atccaaaaag tattctttaa gaaaattttt
    10201 tattagagat gtaattgata taaataatcg tattaaaaaa taaaatacta agtttaaaaa
    10261 tttaaataat ctataaatca attataaaat attatttctt agaaattaaa atgaattaag
    10321 taaatggagg aattgatatg ataataatca gaagtcaaga taaaactaat ttaatgcaga
    10381 taaaccaaat taaaatagat ggtagtcaag tttatgcagt atttgaaagt aaaattgata
    10441 ctgtaaaaat aggagattat gaaaataata caagagctat acaagtgttg gacaatatac
    10501 aaaacttcat tgaaaatgga acaaaatatg attatataac atcaaataaa gtcagataca
    10561 atgttaataa aatatttcag atgccagcta aatagtgagt gtgcatttta atttaataac
    10621 taaagcccac tttcttttgt agtatgaaag agggctttac atatttgtaa atatcgaagt
    10681 acacttgttt tatcaataaa ttatttaata aggtataatg gaccaattaa acaataaaaa
    10741 ttctcctaaa acagcaagta ttaaatatat aatagcttca tttttgtaaa gagaggtaat
    10801 tttttcgctt atgtcatcat caattgtaat agcaatttca tcttgaataa tatttttata
    10861 tatagatggg aaaatatctt ttttctttat aagattaaat aaattattta cacctaccca
    10921 tatcaaatat cctaaaacag cacctaatgt gtccatcatt aaatcatcaa tatctgtagc
    10981 tcttaagtta aacaattgac aaagttcaat tgctaatgaa aaacctaatc cagttaatga
    11041 aacattgata atatttctaa aatttttcca aattaaaggt agtaaaaatc ctaaaggcat
    11101 aaacataatt atatttaaaa tatatgttaa gatgccaact gagtcaaaag gtattagatt
    11161 tatttcatca attctaaaaa gcccactata ccttcctata tcccatatgc taccaattcc
    11221 agctttacta agtgctagat agatatataa taaaaaaata tatacccata ctaaatgtat
    11281 cattacattc ttttcaactt tcaaattttt tcgttttata acgactattt ggtatattaa
    11341 gcatggtaat ataacgcaaa taaatgaata aaatgtccat atcattttat tttgtcccct
    11401 ttcaaattta tgtttttagt tttataagta ctatctttat attaaactgt aagtttta
//
LOCUS       DAEOSP010000127        11322 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.129, whole genome shotgun sequence.
ACCESSION   DAEOSP010000127 DAEOSP010000000
VERSION     DAEOSP010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.129"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..394
                     /locus_tag="KRH54_002532"
     CDS             <1..394
                     /locus_tag="KRH54_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBG8580182.1"
                     /translation="PEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELIKLKE
                     KTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNKRPNG
                     PCVNTNVDIMDIEKAIGFLTGKNILVCN"
     gene            463..1164
                     /locus_tag="KRH54_002533"
     CDS             463..1164
                     /locus_tag="KRH54_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBG8580183.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            1226..2551
                     /gene="rsmB"
                     /locus_tag="KRH54_002534"
     CDS             1226..2551
                     /gene="rsmB"
                     /locus_tag="KRH54_002534"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBG8580184.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            2559..3590
                     /gene="rlmN"
                     /locus_tag="KRH54_002535"
     CDS             2559..3590
                     /gene="rlmN"
                     /locus_tag="KRH54_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBG8580185.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            3610..4362
                     /locus_tag="KRH54_002536"
     CDS             3610..4362
                     /locus_tag="KRH54_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBG8580186.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            4379..6394
                     /gene="pknB"
                     /locus_tag="KRH54_002537"
     CDS             4379..6394
                     /gene="pknB"
                     /locus_tag="KRH54_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBG8580187.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            6502..7239
                     /locus_tag="KRH54_002538"
     CDS             6502..7239
                     /locus_tag="KRH54_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG8580188.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            7337..8221
                     /gene="rsgA"
                     /locus_tag="KRH54_002539"
     CDS             7337..8221
                     /gene="rsgA"
                     /locus_tag="KRH54_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBG8580189.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDENEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            8226..8897
                     /locus_tag="KRH54_002540"
     CDS             8226..8897
                     /locus_tag="KRH54_002540"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBG8580190.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            8906..9547
                     /locus_tag="KRH54_002541"
     CDS             8906..9547
                     /locus_tag="KRH54_002541"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBG8580191.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            9678..10307
                     /locus_tag="KRH54_002542"
     CDS             9678..10307
                     /locus_tag="KRH54_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG8580192.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQVGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            10362..11018
                     /locus_tag="KRH54_002543"
     CDS             10362..11018
                     /locus_tag="KRH54_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG8580193.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
BASE COUNT         4502 a         1147 c         2198 g         3475 t
ORIGIN      
        1 tccagaggat ataataattt taatacctca ttgtattcaa gagaatagtt gtaaattaaa
       61 agttactaat gatatagata attgtaaaga atgtggaaga tgcaacatag gagaattaat
      121 taagctaaag gaaaaaacta atgtaaaaat atttgtagct actggaggaa ccctagctag
      181 aaaaattata atggataata aacctaaagc tgttgtagct gttgcatgtg aaagagattt
      241 gacatctgga atacaagata ttaagaaaat tcctgtttta ggtgtattta acaaaagacc
      301 taatggacct tgtgtaaata ctaatgtaga cattatggat attgaaaaag ctataggctt
      361 tttaacagga aaaaatatat tagtttgtaa ttaaactgtt tttaagtgaa gtgaatatta
      421 agaggatgta taataaaaat atattttagg agagtgttat ttatgtaccc tgtatatggc
      481 ggtttttggg gatttgaccc aacaatggtt gtattaattc cagctatatt acttactata
      541 tatgcacaat ttaaagtgag ttcaacaaca aataagtatt taagagtaaa tacacgtcga
      601 ggatatactg gagagcaaac agcaagaaga gtacttgatt caaatggttt gtatgatgta
      661 aagatagaaa tggttagagg gcatctaagt gaccattatg acccaagaag aaaggctgta
      721 aggttgtcag aagatgtata ttatggaaca tctataactt ctgttgcagt agctgcacat
      781 gagtgtggtc atgccataca acatgctaaa ggttatgcac ctcttcaaat aagaagtagt
      841 ttagtaccag tagtgaattt tgcttcaagt atatcttggt ttttaatatt tttaggattt
      901 attatggctg gtccattttt aaaaattggt atactattgt tttcggcttc agtgttattc
      961 caaataataa cattgcctgt agaatttaat gcttcaagta gagcaatcgt tcaacttggg
     1021 aatttaggga ttatagatga aagtgagtca agacaaagta gaagagtact ttcagcagca
     1081 gctttaacat atgttgcagc agcattggtt tcaatacttc aattacttag attgttacta
     1141 atagctcaaa gaagaaatga ctaaaagtaa agtgggttgt ttagaaacaa ccctttttta
     1201 tgtttaataa aagggagaaa tttatatgga tgcaagagaa gttggattta aagtattatg
     1261 tgatatagaa aaaaatgaca attattcaaa tattgcaata aataagcatt ttaaaaattt
     1321 agaaataagt gatatggata gaggacttgc aacagagtta atttatggag taatagaaaa
     1381 taaatattat ttagattata taatcaataa actttcaaaa ataaaggtaa aaaaaatgtc
     1441 aacttatgtt aaaatctttt taagaatggg aacatatcag attttatttt tagatagtat
     1501 atcagattat gcagcagtta atgaaactgt aaaattatct aagaaatatg ataaaaaatc
     1561 ttctggtttc ataaatgcta ttcttagaaa tgaaataaga gctaaagaga ctataatgga
     1621 cataactgag gaagatagtg taaaatactt atctataaag tattcctata actcttggat
     1681 tataaaaaac tggatagata aatttggaca agaatttgct gaagatttgt tggaagcaaa
     1741 taatgaaaag cctagtatct atataagaac gaatacactt aaaataagta gagaagaact
     1801 tattgaaaaa ttaagtgaaa tgggtattgt atgtttaaaa gttcctatgg tagaagaagc
     1861 tattaaagtc gaaaaaatga aaaatataga aaaaaatgaa ttatttaagg ctggtttatt
     1921 tacaattcaa gatataagtt ctatgatagt agggaaagta attaatccaa aagaaggttc
     1981 atttgttttg gatgtatgta gtgctcctgg agggaaatct actcacttag ctactttaat
     2041 gaacaatact ggtcaagtag tagctagaga tatttttgag cataaactaa agcttataaa
     2101 ctctactgta aatagattgg ggcttaaaaa tgtgtttgta gaaagatttg atgcttctga
     2161 gattgatgaa aatagtataa gtaaatttga ttatgtttta gcagatgttc cttgctctgg
     2221 ttttggtata ataagacgta aaccagaaat taagtataag aaagaagaag agttaaaaga
     2281 catcacttct atacaaaaga aaattttaga aaatgcatct aaatatgtca aagttggtgg
     2341 aactttagta tatagtacat gtacaattca agatgtggag aatataaata tagttacttc
     2401 ttttgttgaa gaaaatcaca actttgaatt tgttccaatt gatacagtaa atgtagactt
     2461 agacaatcaa gataaaggat atttaaagat atatcctaat attcatggta tagatggttt
     2521 tttcatagca aaattaaaga gaataaggta gtgagagaat ggaaaagaaa aagatagtat
     2581 taaaaaattt tactgaagat gaacttaaag agtttatgaa aactatagat gaaaagccct
     2641 ttagaggaag tcaaatattc tcttggatat ataagggtgc aaaaactttt gatgatatga
     2701 acaatatacc aaaaagtttg agaaataaat tagaagaggt ttcatgtata gggcatatag
     2761 atatagaatt aaagttagaa tctaaggtag ataatacaaa aaaatatttg tttttattag
     2821 atgatggaaa tataatagaa actgtgatga tggattatga tagtagagtt actgtctgtg
     2881 tttctaacca agtaggttgt aggatgggat gtaatttttg tgcatctaca atggatggat
     2941 taattagaaa cttagagcca tgggaaattt tagaccaagt tattaaaatt caagaagata
     3001 ctggtaagag ggtatcaaat cttgtattga tggggagtgg tgagccactt gataactttg
     3061 aaaatacaaa acaattttta aaaataatta acgaaaaaaa tggtcttaat atagggtata
     3121 gacatataac tctttcaact tgtggaatag tacctaaaat gtatgagttg gcagacttag
     3181 aaatagctat aaatttagct ttatcacttc attcaccata tgatgaagaa cgaagaaaaa
     3241 ttatgcctgt agcaaatgct tattctattg aagaaatatt gaatgcatgt agatattata
     3301 taaaaaagac aaatagaagg gttacatttg aatattctct tataaaagga gtaaatgact
     3361 cagaaaaaga agcaaaggca ttagctaagc tcctaaaagg tatgctatgt catgtaaatt
     3421 taattccaat aaataaagtt gaagaaagag aatacgaaaa accagataaa gcatttattt
     3481 ataaatttag ggatagtttg gagaagaaca atatacctgc cacagttagg atgtctatgg
     3541 gttctgatat tagtggagct tgtgggcaat tgagaagaaa atacaagtaa tttgtaagga
     3601 ggaagtcgta tggtttatag ttgtgcctcc catataggaa aaataagaaa aaacaatgaa
     3661 gactattgtg agggagaagt tatagataca gaacatggtc caataggaat atttgccata
     3721 gctgatggaa tgggtggaca taaaaaaggt gaagtcgcca gtaaattagc agtagaaaat
     3781 ataattgatt ttctaaagga aaacttatta caacatgata atgtaaaaat agactatata
     3841 gatgatatat taaaacaggc ttataataat gtaaactcaa ttgtgcacaa aaaatctatg
     3901 gaagatatag aatttgaagg aatgggaact acattagtta cggctatagt gtataataat
     3961 gtcttatatg tggcaaatgt tggagatagc agatgttatt tgttaacaga tgaaaaattt
     4021 gataaaataa ctatagacca ttccgtggtt gaagaactta tgatggccca tgtcattaca
     4081 gaagaagagg caagaagaca tccacagagg aatagaataa ctagagcaat tggaacagat
     4141 gatatggttg ttgtagatat ttttaagaaa gaaatcaaaa aaagtgatat aatacttctt
     4201 gcgacagatg gattaactgg gtttattgat gatgaagata taagagattt aatattagat
     4261 tatgaatatg agagaactag taatataagt gaagaattga taagtatggc caatgatgtt
     4321 tctggtaaag ataatgtttc ggttatagtg ataaaagtat aattaggacg gtgatattgt
     4381 gggagataca attttaggaa atcgatatga aatcatcagg aagattggtg atggaggaat
     4441 ggcctttgta tatgaggcta aagacagatt attaaacagg actgttgcac ttaaagtact
     4501 tagacctgaa tttgtagatg atgatgaatt tctaacaaag tttaaaagag aggcagaagc
     4561 agtagcaagt ctttcacatc caaatatagt aaatgtttat gatgttggtg aagatggaaa
     4621 agttcactac attgtaatgg agtttgtaga tgggaagaat ctaaaagaga ttatacaaga
     4681 tgaaggtata ttagatgaat atactgcatt agatataaca aaacaaatag ctatggcact
     4741 tagtgcagcg cacaaaaagg ggattataca tagagatata aaacctcata atattctcat
     4801 atcaaatgaa ggaagagtag taaaagtggc tgattttggt atagccaaag ctgtttcaaa
     4861 ttcaacaatg actaatattg gtagtataat aggttcagtg cattattttt caccagagca
     4921 agctaaaggt aaatttgtaa ctaataatgc tgacctgtat tctctaggta tagttttata
     4981 tgaaatgttg ataggaaaag ttccatttag gggagatagt ccaatttcta tagcacttca
     5041 acacattaat gatgatatag actttacatc agaagaaaaa gttaggattc ctcaaagtgt
     5101 aagaactaca ataaaaaagc ttacagaaaa atctagtgca gatagatatc aaactgctga
     5161 agaattgata gaagatatag agtatataga aaaaaatatt gatttagatt ttataaaaga
     5221 atatgatgat tttgcaacta agaaaattga tgaaaaagaa attaataaag ttgtaaatcc
     5281 aacacttgca aaaccagctc ctgaaaaagt ggttaagcct gttgaagtag ctgatttgga
     5341 tgatgatgaa gattactatg atgattttta tgaagaagat gatgatgaag atgaagaaga
     5401 ggaagagata atgagagcta aaaagaatca gagacctaag agtactccaa gtaagagaac
     5461 taagaaaaag aagaaaaaac aggaaagccc aaaatctaga agaagattaa aagtaattgc
     5521 ggctgttttg atactgatat tgtgtgctca agtattctta gcatataagt ttttatttgc
     5581 tggagggttt ggcaataaat ctttgactgt tcctaatctt gtaaatatga cattagaaga
     5641 agctcaaagt gctgttgaaa aagaaggatt atacctgagt gtaaaaagtg aagagtataa
     5701 tagtgaagtt gatgaaaatt gtataatatc acagacacct gaaggtggaa gtacaaatgt
     5761 caaaaagggt gatactataa atgttgtcgt aagtaaaggt tcaagtcagg catctgttcc
     5821 taatgtagtg ggacttactt tgagtaatgc aaaacaactt attgaagaaa ataacttaaa
     5881 agtgggaact gttaagtatg aatacagctc aatatataaa gaaggtacag ttttaagtca
     5941 gagtccgggt gctggctcat caagagctca ggaaggtgat gaggtaaacc tatatgtaag
     6001 caaaggttca gaaaaatcaa atacacagac tccaacagta ccagataaaa aaccaacaac
     6061 accagagaat aatacaccaa cagaacctgg ttcaaactca ggaaacagtg gtggaaattc
     6121 aggaggctca aattcaggaa atagtggtgg aaactcaggc aataatggaa gtaactcagg
     6181 aggttcaaac tcaggaaata gtggtggaaa ctcaggaggc tcaaactcag gagataatgg
     6241 aggaagttca ggaggctcaa actcaggaga caatggagga agttcaggag gttcaaactc
     6301 aggagacaat ggagatagtt caggaggctc aaactcagga gataatggag gaacttcatc
     6361 aggaactggc gcaaacattg gtaaaacaga ataaatatta ataaaataag agctgtcctt
     6421 ttatagaaaa tattttcttt aaagggcagt ttgttttgta taaaatactg aattggttat
     6481 aatataagga gaaaagtatt tatgaagaag aaaacagttt taattacagg aggagctaga
     6541 gggattggta aagctatgtc taaagctttt gcaaaagaag gttacaatgt attagtaaat
     6601 tttaataagt cagaaaatga agctaaagag ttgtacacta tattaaatga aaaaaatttt
     6661 tctgtaaaac tttttaaggc tgatatttca aatagagaag atgtagagga tatggtagat
     6721 tattgtataa aagaatttgg aggattggat gtacttgtaa acaatgctgg tgtaagtcaa
     6781 gataagttgt ttacagatat aactgatgaa gattgggata atatgatgaa tattaattta
     6841 aaaggaagct tttattgttc acaagttgct ttaaaataca tgatatcaga aaaaaaagga
     6901 aatataatta atatatcttc aatctggggt atatcaggtg cgtcttgtga agtgcattat
     6961 tctataacaa aagcaggcat aataggtatg acaaaggcat tagctaaaga agtaggacca
     7021 tcaaatatta gagtaaatag catagctcca ggtgtcatta atactgacat gttatctgga
     7081 tataatgagg aagatataga cgctcttgtg gaagaaaccc ctctaatgag gctaggaact
     7141 ccagaagata tagcaaattg tgctattttc ttggcatcag acaaatcaaa ttttatcact
     7201 ggtcaggtaa ttagtccaaa cggaggattt gttatttaaa aaaaatgtgg tattatataa
     7261 aaataggaaa gaataacacg atatactact cataaacagt atgtgtttat ataaatttta
     7321 taaagggagg catttgatgc tagaaggaaa aattataaag gggattagtg gattttatta
     7381 tgtagatact tataatggta tttatgaatg taaggctaga gggatattta gaaagcagaa
     7441 aataacacct ttggttggtg atagagtcaa aataagcatt gttgatgaaa atgaaaaaaa
     7501 gggtatctta gaagaaatcg acagtagaga tacagaactt ataagacctc ctattgcaaa
     7561 tgtagataaa gcattaatag tattcgcaat aaaaaatcct aaaccaaact tatctctttt
     7621 agatagattt atagttttgg cagaaaaaga aaatttagaa actgtaataa tccttacaaa
     7681 agcagattta gatgataatg atacattaga aactgttaaa aatatatatg aacttagtgg
     7741 ctataaagtc attcctgtaa gcaatattac taagttaaat atagataaag taaaagaaga
     7801 attaaaagaa aatgtagtag tgtttgcagg accatctgga gttggaaaat caagcttatt
     7861 aaatgaaata gatgaaaatt ttaaacttca aacaggagtt gtaagtgata aaataaaaag
     7921 gggaaaacat actactcgtc acgctgaact tttaaaatta gaatttggtg gaatggttgc
     7981 agatacacca ggttttagtt cccttgccct tgaagatata gaggaagttg aacttaaaga
     8041 ttattttata gaatttgata aatttaatga ttgtaagttt ggttcaaagt gtattcatga
     8101 aaatgaacct aattgtgcta taaaagaagc tgttacaaat ggagaaatat ctaaagaaag
     8161 atatgatagt tatatacagc tgctacatga aataagacaa aataatagta ggaggtatta
     8221 gaaacatgat taaattagca ccatcaatat tatcagcaga ttttgcaaaa ttattagaag
     8281 atgtaagaaa agtagaaagt gcaggatgtg aatatcttca tatcgatgta atggatggac
     8341 actttgtacc aaacattaca ttaggaccac ttgtagtcaa aagtttaaag aaagaaaata
     8401 taaatatggt atttgatgct catcttatga tagaaaatcc agaccagtat attgaagaat
     8461 ttgtaaaagc aggatgtgat ataataactg ttcaccagga ggcttgtgtt catctacata
     8521 gaactataca aaatataaaa tcacatggaa taaaagctgg tgtagtactt aatccagcta
     8581 caccagtaga tacgattaaa catgtactac ctgatttaga tatggtatta ttaatgtctg
     8641 ttaaccctgg atttggagga caatcattta ttccttgtgt tttagataag attaaagagt
     8701 taaaagctat tatagatagt caaggattaa acattgatat agaagttgat ggtggtataa
     8761 gccctaaaaa cgtagccgaa gttgtacaag caggagctaa tgtaatagta gcaggttctg
     8821 caatatttgg aagtgatgac attcaagaaa cagttaattt atttagaaag aatgcttctc
     8881 ttgaagaatt agtttaaatt taataatgaa aatctgtatt gttttaaatg gcgaaattga
     8941 agattacgaa gttacaagag atattataat taaggaatgt tatgactgca taatatgtgc
     9001 agatggaggg gcaaatcaca cttataaaat ggaaataatg ccagattata tattaggaga
     9061 tttagattca gtagaagaag aaaagataaa cttctataaa aataaaggtg ttaaatttga
     9121 aaaatttcca tctaaaaaag atgagactga tacagagctt tgtctttttt tagctaagac
     9181 acttaaagca aatcatattg atttttttgg tgctttaggg ggaaggattg accacactct
     9241 tgcaaatata aagctattat attatttaaa agaagatggt atatattcta ggatactttc
     9301 tgataaagaa gagatgtata tagttgaaaa tgaggaaatt tcattgtatg gaaacccagg
     9361 ggacactatt tctgtaatag ctataaatgg tgatgctaag ggtgtaacac ttactggctt
     9421 agaataccct ctcgataatt attatatgaa atactctgta cctattggaa tatcaaatgt
     9481 tatgttgagt aattcttgta agattaaagt agagcaaggc tgtgtacttg tagttagaaa
     9541 tttataaaat ttgattttga caattggttt tatattgtag agcagataca atcaagtaaa
     9601 atatatttat gattgtatct gctttttagt atataattat aaatatataa taaactttga
     9661 cataaggggg attttttgtg gtagatagta ttatttttga tttagatgga acattgtggg
     9721 attcaactga agaagtatgc aaagtctggc aggatgtttt aagtgagcat aaggaaatag
     9781 aattaagtgt cacaaaagat ttatttagaa gtcttatggg gctttctttt gaggaaatag
     9841 caaatcgttt atttccaaat ttaagtgaag aagaaagaat gaatttattg aatgaatgtt
     9901 ctttgaaaga atgtgactat ttatctgaaa atggagctaa attatttgat gatatagaag
     9961 atactcttaa attactatct aaaaaatata aattatttat agtaagtaat tgtcaagttg
    10021 gatatataga atctttttta aaagcacata aactagaaca gtattttata gattttgaat
    10081 gtcctggtaa tactggtttg cataaaggag aaaataataa acttataatt gaaagaaata
    10141 aattaataaa tccaatctat gttggtgata cgcaaggtga tgcaaatagt gctaagtttg
    10201 taggtatacc ttttatatat gccaaatatg ggtttgggaa tgtagatgta tatgattatt
    10261 ttatagattc atttaaagat ttgttagaac atgatatatt aaaataagat gagtttaaaa
    10321 attatattaa aataataaaa aagttatggg gggatttaaa aatggaatta caagatacta
    10381 tttttaaaag acaaagtgta agaaagttta aaaatcaaga tgtatcagat gaagatattt
    10441 taaaaatgat aaaagcagca ggtgcagcac catcaggcaa gaatattcaa aactggcatt
    10501 ttgttgttat aaaacgccgt gatttaatgg aaaagatagc agatgtgata actaaaaagc
    10561 aacaagaaat acttgtagaa atggataaag tatcagtgga taaggctaat agatttagaa
    10621 aatttgttaa aaattttaca ctattttact taaaagctcc agttttagta ttagttttta
    10681 caaaggtata taatccatca ggatattatg aattagagct tatagatgca cctaaggaga
    10741 ctatagataa attatttata agaaatccag gtatgcagag tctaggtgct gcaattgaaa
    10801 actttacact atcagcaatt gaacttggat atggttcttg ttggttgaca agtcaaaact
    10861 atgcagcaga tgagatagaa gctgttttag aagcagaaac tggttttgaa aagggagagt
    10921 attttttagg tgcaatgtta gctcttggag tgccagagga caatttgaag agtccatcta
    10981 aaaaaccagt agaagaaata tgtacattta taaaataaaa gtcaaggaca ttttaaaatc
    11041 gaagtaaatt aaaaacaaaa taaattaaaa ataaatattt aaaaaattaa aaagtaggtt
    11101 ttattgatat taattttata tttcaaatca ttgatttaat taatacaata agaactactt
    11161 tttttgtata ttaattttaa atatatttaa atctttagaa aatctttaga aaagtatatg
    11221 taatatctta agtttttaat tttaatatta agtatataaa acaaaatgaa aaaatattaa
    11281 atttaaacta gaaggaggtt tctaagatgg acattaaata ca
//
LOCUS       DAEOSP010000128        11061 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.130, whole genome shotgun sequence.
ACCESSION   DAEOSP010000128 DAEOSP010000000
VERSION     DAEOSP010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11061
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.130"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            41..1522
                     /locus_tag="KRH54_002544"
     CDS             41..1522
                     /locus_tag="KRH54_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG8580194.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTNINETFARVSAD
                     SFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLLEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            1555..4992
                     /locus_tag="KRH54_002545"
     CDS             1555..4992
                     /locus_tag="KRH54_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBG8580195.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTIELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDKKKALESIQHQLNTKGIAVYQHRIRRRNGNIIWVSIKGRLIVDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFINNKVSRLDLEIKLGETCYKSLLKSDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            5010..5756
                     /locus_tag="KRH54_002546"
     CDS             5010..5756
                     /locus_tag="KRH54_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBG8580196.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQGKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5705..6013)
                     /locus_tag="KRH54_002547"
     CDS             complement(5705..6013)
                     /locus_tag="KRH54_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG8580197.1"
                     /translation="MNFFKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIVKYKT
                     SYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
     gene            6817..10959
                     /locus_tag="KRH54_002548"
     CDS             6817..10959
                     /locus_tag="KRH54_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBG8580198.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTSPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGTGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIVNPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWT
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFTENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NTSTSEAEVSSSADISVVKRSNQIVVVPGDVLDYTIEVRNAGPSTTQNVTLTDNIPAS
                     ILSPEYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNPDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTL
                     IDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVILLRGIIKSTCLDRLTNI
                     AEITSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDF
                     WC"
BASE COUNT         4469 a         1327 c         1756 g         3509 t
ORIGIN      
        1 aattatatta taattgctaa agagagtgag gtgtagctat ttgaaaaaaa atagattaat
       61 agcaaagaat atatcaatag catttatagt actattcttt tttagtgtat ttacattttt
      121 ttatgtaggt aatattaata gagtgttaga atatgaaaca aatgacataa taacagttac
      181 aattgctgga tggattatac tttcattttt atttttaggt attataattt acatattata
      241 ttcaaaagca aatagtcaaa aaactataga aaaagtagca tatacggatt ttgtaacagg
      301 ttattccaac tggagaaagt ttgaattaga tgttacaaat ttattaaaga aaacatcaca
      361 aaataataaa tatgcaatgg tcatattcga tattgataaa tttaaagcaa ttaatgatat
      421 atatggtcat aaaaagggaa atttaatatt aaaagatatt gcagatactt taaatgaatt
      481 gactaatatc aatgaaactt ttgctcgtgt aagtgctgat agttttaata tattattaac
      541 atataacaaa aaagaggata taataaatat tatcaaaaag attatggcaa ataatgaact
      601 tgtaaactta tcttttggaa tttatgaaat caaagataag gatttatctg taagtgtata
      661 tagcgataga gcttcacttg caaaatcatc aataaaaaat aatagtgatg taaattttgc
      721 attttttaat gataaattaa gagagaaatt attacttgaa gataagatag aaaaagaaat
      781 ggaatatgct cttgaaagtg ggcaattcgt aatgtattta caaccaaaat acaatattaa
      841 attagataaa ttttgtggaa gtgaagccct agtcagatgg caatatacag aaaaagaggt
      901 catatatcca ggtgatttta ttcctatatt tgaaaaaaat ggatttatta gaaagattga
      961 tatgtacata ttagaacaag cttgtaaaga aatccggtct ttatttgata aaggaatttc
     1021 acctttacct atatctgtaa acttctcaag agttgatttt tttaaaaaag attttattga
     1081 aaacatagta aatatatgtg atagatataa aataccatat agtttaatag aaattgaaat
     1141 tactgaatct agtatgtttg gagatactga tactctattt aatgttagta gaaacctaca
     1201 ggatattggc tttatagttg caatggatga ttttggctcg ggatattctt ctgtaaatat
     1261 gcttaaaaat ataccattaa atgtaataaa attagataga ggattttttg ttgatgataa
     1321 agatgttgat aagagtcaaa ttgttataaa aagcatagtt tctctcatta agcaattagg
     1381 aataagggtt gttgctgaag gaattgaaac aagaagtcaa atagaaatgt taaaaaaagc
     1441 aaattgtgat attgttcaag gatattattt ttcaaaacca ttaccaatta aagaatttga
     1501 aaaattagtc tataaaatat agtcagcgag tcttaaatgg gaggtggtga ttaagtgata
     1561 agagcaaaat cgttaaaaaa tgtactgagt attgttttaa tatttttatt tacaataatt
     1621 tctttttctg attttgcatt taaaactaat gtccaggaag aagaaaatag taaaattaat
     1681 ttaagggaaa tatccctaca aagttccaat gctataaaaa taaaattaga tagttctcta
     1741 tcctttatta aaagtgtggc aaatatggtt tcttatttta atactataga taactcaaaa
     1801 atagttccat tattagctaa tatatcaaaa gaatctgact ttcaaagaat ctctattgta
     1861 tctcctgatg gaataagtca taccagtgat ggcaaaatta tcaatgtgtc aaagtatcag
     1921 tattttaaga aagctattaa aggagaaagt aatatatctg aagttgtgaa atctccaatt
     1981 gatggaaaag atacttttgt tatagctact cctgtattta ataagaataa caaaataatt
     2041 ggtgttttat acggttctta ctacaatgat aaattagctg aatttataga tataacaagt
     2101 tttaatagtg aagggtacat agatatattt gaatctagtg gtcaatttgt tttaaaatct
     2161 tcacatacaa acaaactgat taagtcaagt agtaacatat atgatatatt taaaaaagct
     2221 tcttttgatg atggttatag ctataaaaaa atatatgaag atacaaaaaa taacaagagt
     2281 ggctttacct catatgaata caaaggagaa aatcgttatg ctacttatac acccattggt
     2341 gtaaatgact ggtatgtaat ttctgtgata ccacaatata ctgtaagaag aaacattgat
     2401 aaaataaatc aattagcatt ttctcttatt ttcaaggtat tcttattgtt tattatgctt
     2461 ataatatata ttatttataa aaacaataaa tctcagaaag atatactaaa agctaacaaa
     2521 gaaatctcat ctattacaaa tagcattcct ggaggagtac aaaagtgtac cctgggtgaa
     2581 acatgcaata ttaatttttt aagtgatgga tttatagagt tgtttggtta tacaagagaa
     2641 gaaataaaaa cattgtttaa taatgatttt tatagtttaa tacataaaga tgatgttgaa
     2701 agaataaaaa aagaaatttt taaacaactt tacaaaaata aaactattga attagaatat
     2761 aggattataa caaaaaatgg taatacagta tgggttttag ataaagggga gcttataaca
     2821 gaacctgatg gaaaagaata tttttgttgt gtaactgtag atattactag aacaaaacaa
     2881 ttagaacaag agatggaaca aaaaaatcta gaacttgaaa tgatatcaga aaatgtttta
     2941 ggtggtataa ttataacaga atttaatgat gattttacta taaaatacgc taactctggt
     3001 tatctttcaa tgattggata tacacgtgaa caattagaaa atgaacttaa atacaaagct
     3061 tgtaatcttg ttttttatga ggataaaaag aaggctcttg aaagtataca acatcaatta
     3121 aacacaaaag gaatagctgt atatcaacat agaataagaa gaagaaatgg taatattatt
     3181 tgggtttcta taaaaggaag gcttattgtt gatgatgatg gtattgaaaa aggtatttgg
     3241 attctaaatg atataacaga tttaaaaaat gcagaacaaa aacttaaaat aaatgaagaa
     3301 agatttagaa ttgcattaaa gcaaacatca aacactatat ttgagtatga tataaaaaca
     3361 aagtcacttt taaatactag taaagagctt gataaatata atatttataa aattactaaa
     3421 aacgttccat attctctagt agaatcagga gttattcata aagattttag tgatgatttt
     3481 ataaacatgt atgaaaatgt aataaatgga gcaaaaaaag caagttgtgt agttaagatg
     3541 aaacttccag atgaaaatta tatatggaat aaagttacct tgactactat ttttgatgaa
     3601 gatggtaatc ctgtaagagc aattggtata caggaagata taagcaaaca aaaggaagca
     3661 gagataagat acaatcaaga aggtaagtat cattcagcta tattgtcgga agcaatgata
     3721 acttatgaaa taaatgtatc aaaggatact tttatcaaag gaaatgaaag ttggcaaaaa
     3781 ccatttggaa ttaagcctga caataactat tctcaaatgg taaaaactat agcaaatagt
     3841 attgtacata aagacaataa agaaacattt ttaaatacat ttttatgtga aaacttattg
     3901 gatgcatata aaaataatgt gactgaaata aaattagaat atcgaagaat gaatcaagaa
     3961 aataaaatga tttgggtcat ttgtacaatg cacttaataa aagattctat tacgaacgat
     4021 ataaaagcac ttgcttatgt taaagatatt gacaagcaaa aaaatgaaga aattagatta
     4081 aaattaaaag ctgaaagaga tactcttaca ggattatata ataaaggtac tacagaaaaa
     4141 ttgattaatg atttcttgaa ctctgaggaa gcaagtttag gaatgcatgc atttttaatt
     4201 gttgatatag ataattttaa atctgtgaat gataatttag gacatgtatt tggagataaa
     4261 gtattatttg aagtttctaa aaacctaaaa cctatattta gaaaagatga tatagtaggt
     4321 agaattggcg gagatgaatt tatagtattc ttaaaaaata ttcagtcttt tgagaatatc
     4381 aaaaagaaag cagaaaaaat atgcgatata tttagaaatt cttatacaga aaataaaaag
     4441 acatataaaa tatctggaac agttggaatt tcatttgtac cagaacatgg aactgtattt
     4501 gaagaacttt ataaaaaagc agacatagca ctttattatg caaagagcaa aggaaaagat
     4561 aagtatgcac tttataatga ttgtatctac caaaatgaac ttgaaaataa tataagtgat
     4621 aataatataa aattagcaga taaatgtgag aaatccaaac cagtacttaa agaaattctt
     4681 gattctattg ataattatat ctatatagtg aatccaaata cttatgaact tctttttatc
     4741 aataataagg tgtctcgttt ggatttagag ataaaattag gagaaacctg ttataaatca
     4801 ctattaaaat ctgatagacc atgtaataat tgccctctta acggattaga agaaaataag
     4861 cattcaaaat tcaaaataga gacaaataca tttggtaaaa atatcagtat agcaagttgg
     4921 attaattgca tagatggaga atacaactgt cttatagagt gtatagattc ttctttatac
     4981 aaaaataaat aaaaaaggac tatgaagata tgttaaatag tataactttg atacttggta
     5041 taatatttac aatttactat ttttatctca aaatagtttt tggaggaatt tcatttagtg
     5101 agattttctt agttgtagga attgtactaa ttatctatca aatatttaag aaaaaaatta
     5161 aagaaaagaa ggttttttat agggtgttaa aaataataat cagcattttt ttaatagtat
     5221 ttgtagttac tgagagtcta attatttttt atcccaaaaa tagtttagaa tcaaaaagtg
     5281 attatctctt aattttagga gcttctgtaa aaaagactac accaagtaca accctgaagg
     5341 gtaggttaga cactgcattg aaatacctaa aaattaatga taattgttat gtggtagtat
     5401 ctggtggtaa aggaagtgga gaaaaaataa ctgaggcaaa agctatgaaa gattatctta
     5461 taaaaaatgg tatagataaa aataggataa tagaagagga taaatccacg aatacttatg
     5521 agaattttaa atattcaaaa ttgaagatag aagaacatag tcaaggaaaa ctaagcgatt
     5581 taaaagtaaa aatagtaaca acagattttc atgttctcag aagtaaaata ttagcatata
     5641 gaaatggata taaaaatacg tctttttatg ctagtaaatc aaaactttca tttgtaccaa
     5701 cttactatac aagagaattt tttgcattat ggaaaacaat catttttgat agataataaa
     5761 taaaaagact agatataaac tcatatctag tcttttctat tcccttaatt ctaatccatt
     5821 ttccaacttt acttgttgta gaaatttcaa cattgggatt tacaagtaat tgcttataag
     5881 aagttttata ttttacgatt ccaaaatata attttccttc ataatccatt ataaatccga
     5941 atggtcttac tcttggttta ttatcttcaa ctgtagcaaa atagaatgtt gagttttctt
     6001 taaaaaaatt cattattttt tcatgattaa atcctctttg tatcttatac taatataatg
     6061 aatataaata aaatattcat aatactaata taaatcgata taaaaacaat tacaatatta
     6121 tattgattat tcaaaattat atataaaatt cagaaaaaaa ttttagttta cataataaaa
     6181 ataaagtcaa ctaaaattat tgattttaga ttaaagtacc ggattaagca tttaagtaga
     6241 ttttaagctt gatttgatta gaaaggtaca aactatcacc gaaaaaaatt aaacccattt
     6301 aaaaagcaat ataaaagctt gttatttttg agaaaaatag accctctaaa ctcaattaga
     6361 ttaaaaataa aatacagaaa attatatctc taaatatctt aaataattaa gtttaaattt
     6421 tacgatattt tgtttgatga tttataatat aaaagcatac tcttaatctt caaaatttaa
     6481 aaagagtaaa aaataacaaa aaaatcactg acatttcact gacaaattgt aattttgtta
     6541 ttataaatac attgaaaata cttgattatt gaaaaatggc ataaaataat acattttatg
     6601 ccatttgcta ttatttgata gtttttaact tatgacataa gatatttttt aacatataca
     6661 ataaaatata aaaaaattga tattgcaaat aaaaaaaaat acatattaga tgttatagaa
     6721 aaaaagcgca cttataggct taaaatgtca cataaaagag ttttaaagaa taatatatta
     6781 tgaacacaaa ttttgtaatc acaaaggagt gataatatgg caacagtatc aggacaaata
     6841 gtttttgata gaaatagaag tgctaccatt gattctggag actcaggaat agccaatata
     6901 ccagttgtat tacaagatat agcaactgga gttagacttg tggttctcac agatgcatca
     6961 ggaaattatg tttttactaa tgttccaaat ggtaactata gaattgtaga agcttttgga
     7021 actacaggag gagtaccaac accaggaaat tttgcaacag cgatagtagg tccagttcca
     7081 attgcagaaa tgccacctat tagttctgtg acaagtcctc ctattggttc aacaaactta
     7141 gatggtacaa cacctaacac actattaata acagtagcag gttcagatat aacaaatcaa
     7201 aatatattaa atgggcctgt gatttataca cctatagaga atatattgga ttcttgcgta
     7261 tctgtatcta atataaacct tattacagat gcagataacg gaacattcgg tttttttcca
     7321 ccaggtacac caagtaatac aggacctgaa atagaacctt acccaaatgt gacgccagat
     7381 tttacgtatg ttgtaccaga cccaactaag tttacacctt tagatggaga atatacagta
     7441 caaaatatta tgacaaatgc aatgagtaat gttataggag cttggtggcg tattgctgac
     7501 catactacag gaaatgaaac aggaagaatg atggtggtaa atggttttaa tcctggagct
     7561 gtatttttta aatcaacagt acaagttaca cctaacacta attatttatt tagcacttgg
     7621 attttaaacc tttttaaggt aacaggatat ccaccaccac agctaggtgt aagaatattt
     7681 gaccaaaata ataatatact atatcaagca acattaggag ctcaaattcc agttaataca
     7741 aacgcaccag aatggaaaga agtaggtact gtgattaatt ctcaaaataa taatcaactt
     7801 acagtagaat tttttagtga aggagaaagc gccattggaa atgactatgc tatagatgat
     7861 gtttctttaa gagaaatagc tgttccaact tttactccaa taaaaacaag taacacagaa
     7921 actgctaatg taggtgatat aatcacatat acagttacat tagataatac ttgtgatagc
     7981 cctttaacta atgtattttt tagggattta attccaaatg gtttagagtt tatacctgga
     8041 actgtaacgg ttgatggagt atctgtacca ggagtagacc ccaatacagg tttcccactt
     8101 ccagatattg gaggaggaac aggggttgaa gtaacttttg atgttgtggt tgaaagtatt
     8161 cctaacccaa atcctactaa taatatagct actatagatt actcctatac acctgtagaa
     8221 ggcggtatac caaatgattt ttcagtagat tctaatccag ttcctgttga agtaatctca
     8281 gcagatattg aagttacaaa attatctgaa cctactatag tgaatccagg agaggaatta
     8341 atatacacta taaaagtagt aaataatgga ccattcccat ctgaaaatgt ggttttaaca
     8401 gatgatgtac cagcttctat tgtaaatcca gagtattcat tagatggagg agtaactttt
     8461 caaccatgga ctggaagttt aaatataggg acattagaag taggagagac aagagttatt
     8521 ataattaggg gtattgtaaa tccatcaaca gtaggaatta taacaaatac agctgtggta
     8581 tcttcaacta cagcagaccc aaaccttaat aataatacat ctacaataga gacagaagtg
     8641 aatttattag cagatatttt agtaatgaaa acagcagaac ctaattcagc tgttccaggt
     8701 actttattga gatatactat tcaagtagaa aacttaggac cagcaaatgc tgaaaatgta
     8761 atattaaatg atgatattcc agcttctatt ataaatccag aatattcatt ggatggagga
     8821 gcaagttttc aaccatggaa tggaagtctt aatataggaa ctttaaactc tggaatttca
     8881 ttaacagttt taattcaagg aacagttagt ttaaattctt cagaatatat agtaaataca
     8941 gcaacagttt cttcaactac acctgaccca gatttaagca acaatatatc taccattatt
     9001 acaccagtta acccacaagc aggtatttct attataaaag tagctgacga ggatgtagct
     9061 gttccaggag aagaatttgt atatacaatt gaaattttta atgaaggacc atctaatgct
     9121 acgaatgtag ttttaacaga tgatatacca gatgttattt taaatccaga atattcattg
     9181 gatggaggag caactttcca accatggact ggaagtctta atataggtac agttgcacca
     9241 gggcaattaa taagaattat cataaaggga ttagtaagtt caactgctac aggagatatt
     9301 actaatacag cagaagtaag tgcagatgta ccagaaccag ttacggattc atcaacagtg
     9361 acaactccta tagttccatc agcagatata gaagtaataa aaactagcaa tatggataca
     9421 gcagttccag gagaaacttt cagttatact ataacagtta caaatttagg accatctgct
     9481 gcacaaagta tcgttttgac agatgatata ccagatgtta ttttaaatcc agagtattca
     9541 ttggatggag gagtaacttt ccaaccatgg aatggaaatc ttagtatagg cactttggat
     9601 gctggagaaa taagaagtat tataataaga ggaacagtga gccagacagc agtaggtaca
     9661 attataaata cagcaacaat aagttcacct acacctgacc caaatccaga taataatact
     9721 tcaacagatg aaacagatat aagtccatta gcagatattt ctgtgataaa aggcaacgaa
     9781 ccagttgcta taccaggggg aagatttatt tatggaattg aaattgcaaa tgcaggacca
     9841 tcttttacag agaacgtaac tttaacagat aatataccag cttctatttt aaatccagag
     9901 tactcaatag ataatggagt gactttccaa ccatggaatg gaagccttaa tataggtact
     9961 ttagatgctg gagagataag aaatatcata ataaggggaa cagtaagtca aacagcaata
    10021 ggcacaatta taaatacagc aacagtaagt tcaactacac ctgacccaaa tctaaataac
    10081 aatacttcaa catcagaagc agaggtaagt tcatcagcag atatttctgt agtaaaacga
    10141 agcaatcaga ttgttgttgt accaggagat gtactagact atacaattga ggtgagaaat
    10201 gcaggaccgt ctactactca gaacgtaact ttaacagata atataccagc ttctatttta
    10261 agtccagaat attcaataga taatggagtg acttttcaac catggaatgg aagtcttagt
    10321 ataggcactt tagatgctgg agagataaga aatattataa taaggggaat agtaagtcaa
    10381 acagcaatag gtacaattat aaatacagca acagtaagtt caactacacc tgacccaaat
    10441 ccagataata atacttcaac atcagaaata gatataagtt catcagcaga tatttctgta
    10501 ataaaattag caaataaaac agaagcatgt gtaggagagc aaatagattt tgtaatagta
    10561 gtatcaaatg caggaccaga aaatgctcaa aatgttactt taatagacaa tgtttcagat
    10621 aagcttaaga aagcagtatt ttcacttgat agaggtgtaa gtttccaacc atggactgga
    10681 agtcttaata taggtacact accagctgga actttaagag ttattctatt aagaggaatt
    10741 ataaaatcaa catgtcttga tagattaaca aatattgcag aaattacttc aacaacacca
    10801 gattcaaacc ttaacaataa tatatcaaga gtgcaagtag aaataaaaca atgttgttgt
    10861 aatgattgct gttgtaacaa ctgtagtaat tgttgttgca atgattgtgg ttgtaaagat
    10921 tgttgctgta attgttgtaa aaatgacttt tggtgttgat tttataaaat aattttgtat
    10981 accttaaatt aaactgaaat aaaaaataaa ttcacatatg agtaaaatat tttataaata
    11041 ttaataaatt ttctatcttt g
//
LOCUS       DAEOSP010000129        11056 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.131, whole genome shotgun sequence.
ACCESSION   DAEOSP010000129 DAEOSP010000000
VERSION     DAEOSP010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11056
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.131"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(106..957)
                     /locus_tag="KRH54_002549"
     CDS             complement(106..957)
                     /locus_tag="KRH54_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBG8580199.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(1006..2175)
                     /locus_tag="KRH54_002550"
     CDS             complement(1006..2175)
                     /locus_tag="KRH54_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBG8580200.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            2646..3563
                     /locus_tag="KRH54_002551"
     CDS             2646..3563
                     /locus_tag="KRH54_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBG8580201.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(3692..4621)
                     /gene="pfkB"
                     /locus_tag="KRH54_002552"
     CDS             complement(3692..4621)
                     /gene="pfkB"
                     /locus_tag="KRH54_002552"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBG8580202.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNTCRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            4888..5613
                     /locus_tag="KRH54_002553"
     CDS             4888..5613
                     /locus_tag="KRH54_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8580203.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITTGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            5696..6850
                     /gene="nagA"
                     /locus_tag="KRH54_002554"
     CDS             5696..6850
                     /gene="nagA"
                     /locus_tag="KRH54_002554"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBG8580204.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNNTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYIPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICMYKVNK"
     gene            complement(7033..8190)
                     /locus_tag="KRH54_002555"
     CDS             complement(7033..8190)
                     /locus_tag="KRH54_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBG8580205.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            8344..8913
                     /locus_tag="KRH54_002556"
     CDS             8344..8913
                     /locus_tag="KRH54_002556"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBG8580206.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNS"
     gene            complement(9095..9511)
                     /locus_tag="KRH54_002557"
     CDS             complement(9095..9511)
                     /locus_tag="KRH54_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580207.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(9737..10831)
                     /locus_tag="KRH54_002558"
     CDS             complement(9737..10831)
                     /locus_tag="KRH54_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580208.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
BASE COUNT         3849 a         1781 c         1344 g         4082 t
ORIGIN      
        1 catcaattaa atgaaaaaaa tacaccattt acatataatt aatataaatt tttcatataa
       61 taaatatcct aaacttatct aaaataaatt taggatattt tatttttata ctctatttac
      121 agacccacac actttaatct tttctttcac aaaatccttt actgattgaa gccctgctaa
      181 tccatacttt ttagggtcta taacctcaga atcttcatct aaaacttttt taaccccttt
      241 actatatgca attcttaatt cagttgcata gttaactttt gcaatacctc tttttatagc
      301 ttcttttata atatcatcat gtattcctga acctccatgc aatactaaag gtattgaaac
      361 tacattttta atttctgata acctatctaa atctatttta ggttcaccct tatacaatcc
      421 atgtgcagta cctatagcaa tagccaatga agatattcct gttctatcga caaactcttt
      481 tgcctctttt ggatttgtat aatcattact attcccgcca tctaaatcat cttcttttcc
      541 accaactttc ccaagttctg cttccactgg tataagagaa ggactacatg cttttactac
      601 tgatttagat atagatatat tattttcaaa gctttcatga gaaccatcta tcattattga
      661 tgtatatcca cttcttaaag ctttcattgc aaggtcaaaa cttgaaccat ggtccagatg
      721 cattgctaca ggtatattta ctctttctgc agcaaccttt gcatttgcta gataataatc
      781 aagacctgca tattttacag tagatggagt agtttgtaat attactggag aatttaactc
      841 ctcagcagct tctataacag caattaccat ttccatattt tctacattaa atgctcctac
      901 tgcataccct tcatcttgtg cctttttaag cattgatgtt gttgttacta atgccacatt
      961 gataccctcc caaattattt agttataata ttttaatctt atcttttaat attcatatat
     1021 tgttacacct tttactactc gatttactgt tcctgtagct gatggagtat ctggcttatt
     1081 tccaaccttt actgatacaa acaatgctat agtttgtgca aacaatatgt atggcattgc
     1141 aagatacaca tctggtaatt tattatactt ttctttaaac tcaaactttg ttcctgagaa
     1201 gttattttca ttttctactg acactgcaca agtgaattta gcaattttat ccctttttat
     1261 ctcctctaaa acatctaaat catattttct tgtatataaa cagttagata caaattcaaa
     1321 tacaagtgta ttttcatcaa taaatgattt tggtccgtgt ctaaacccca taggagaatc
     1381 atacactgta gatatttttc ctgctgtaag ttctaaaagt tttagttgtg cttcttgtgt
     1441 caaaccacct aatcctcctg aacctaaata tacaactcta tcaaaatctt tatttattaa
     1501 ctcatgtatt tcatcttttc tatctataac atttcttccc atttcaatta ttgcatttat
     1561 atatgacttt tctacatcat cttctaaact atcgaatatt aacatagcag ataacatcat
     1621 acacgaaaaa cttcctgtca tggcaaagcc ttcatcattt gattttgatg gcattaataa
     1681 caagtaatta ttttcgtcgt ttttagtcat cttagccagt tctccttctg gattacaagt
     1741 tatagctaaa tgataaaaat tatctacaat cttattccct aaatttaaag ctgctaaact
     1801 ttcaggacta tttccacttc ttgcaaatga aattaataat gttggtatat ctttttttaa
     1861 gtaatcatat gggtttgaaa ctatatccgt agtaggtatt gcttcaaata tgtattttct
     1921 tatgtcattt ttatttttaa ggtaaggaag tattgaattt cctatgtaag ctgaagttcc
     1981 agcacctgta aatataactc taaactgccc atgattatta cttattgtat ctatgaatct
     2041 acttaatttt tctttgttag atttatatat ttcataagtt tctaaccata attcaggctg
     2101 ttgttttatt tcattagttg ttattattgc acctaaatct tttagttttt gttcttctaa
     2161 tttaaacatt ttcattctcc tttcatactt gtaacgttta catttggtaa tgaggttata
     2221 accactttaa actataaaaa aatatactct tttataattt tttgcttaga ttttttagta
     2281 ctacttaaac tcactataaa atatacacta tagattttaa ttaattttat agtttatctt
     2341 taaaaattta ataaaatcat actctatata ctttagtatt tttattttac gataaaagct
     2401 tgttcagacc aaagtggtaa ttaccactaa tccaaatata aattaatttg aattttttgt
     2461 caatacataa aatatttata tcaatcaatt ttatggatat cctacaaaat tactttatca
     2521 atatttctta tttcgctaat ttacttatat aagctttaaa tttatttttt gatacaaagt
     2581 ttacaaattt atgtgtctga attataatca attctgtaaa tataaaaacc ttaggagaaa
     2641 gtattatgaa aaatataaaa atgggaatga taggtcttgg ctccatagct caaaaggcat
     2701 atttacctat attaactaag tctgagagat ttgagtttgt aggagctttt actccaaata
     2761 aagttaaaag ggaaaaagtc tgtagtgatt atagaatcat gccattcgat tctattgaat
     2821 ccctagctaa aaaatgtgat tgtatttttc tacatagctc tacagaaaca cactacgaaa
     2881 ttataaaaat attattaaat ttaggtgttc atgtatatgt agacaaacct cttgcttcaa
     2941 cagtaagtca aggagaagaa cttatcgagt taagtacgaa aaaaaactta aatttaatgg
     3001 ttggattcaa ccgtagattc tgtcctatgt acaaagaaat taaaaataat gctacagaaa
     3061 ttgtatctat caatatatgt aaacatggat taaattcctt aagaaatgtt agattcgaca
     3121 gtactcttat agatgactat atacatgtta ttgacactgc tttatggtta gcaaatgaag
     3181 atgttgaaat tagtggagag gatttatttt taacagacaa taagaatctt atatttgtaa
     3241 gtcataaact taaaggtaaa aatttctcta taaacacctc tatgcataga gattctggaa
     3301 caaagttaga acaagttgaa attctttcaa agggtaagat tcaaagagtt aagaatctta
     3361 atgtactgga aatagaagaa ggtggaaacc taactttaaa gcaaagtggt gcatgggtaa
     3421 atatattaaa acaaaagggt tttgaggata tatcaaatca ttttattgat tgtatagaaa
     3481 ataatataaa accagctatt aatggagaag agtgtatcaa agctcaaaga ttactagaga
     3541 aaattataaa ttcagtaaaa tagatattta ttataaaaat aggttaaaaa tgaatttttc
     3601 taacctattt ttatataaga ctatatcttt tatattaaaa ttaaaatcta ttacaacaac
     3661 attaaattta ttatattccc attaaatgta tttaacttaa cttttttact tcaactaaat
     3721 cttgatattt atcaatttct cccaaatcta caaatccagt ttccatattt aaagcattac
     3781 ttataccaca agcatttgca aatttaagta aattttctat tttataaccc cttaaaatac
     3841 ctacactaaa tccagccact gtgctatctc cactacctac agtatttata cactttacaa
     3901 ctggtacttt tacatcatat acattatctt cgtttaaata caccatacca tcttttccta
     3961 aagatataca tacattctct gcacccattt caataagttt ttttcctgac aagataattt
     4021 cctctcttga ttctatattc attcctaata aatgtcttat ttcatctata tttggtttta
     4081 ttaaatatgg ttttgatttt aaagctattt ttaatgcttc tcctgatgta tctaatataa
     4141 actttatatt atttgctcta catgtatttc ctatcttttc ataatagtct atcggcatat
     4201 tctcacaata actaccagaa gctaccagta ttttagtatt ttttagtact tcctctatat
     4261 cattttcaaa tctctctaaa tcgctttttt ctactattgg tcctttttct aaaaattctg
     4321 tactaacttt attgtcctca attatgttta agcaatttct agtttcctgc tctatttttg
     4381 taaatttatt ttgtatatct agttttctaa gttcattttt tataaattct ccattaaatc
     4441 cgcctagaaa tcccatagct aaaggatttt cttttaaaat tttacatacc ttagctacgt
     4501 ttaatccttt acctccagca gttgcattct tacttactac cctttgcacc tcacctatgt
     4561 ttatattatt cactctatac attctatcta tagatgggtt gaatgttatt actgtaatca
     4621 tactagattc tcctcattat taatttttac ttttctaaac aataaattaa tctgtaaaat
     4681 aaaaaatacc tcatataata tatccaaagt ggttattacc aatttgaata taaattattt
     4741 tacttgtttt gtcaatgtac cataatttta taataaatta atagccttac ttattgaatt
     4801 ttgattaaac tttattcatt gacataaata attctatatt ctataatcaa attgtggtaa
     4861 taaccactta gaaaggacta tatttttatg ataaaaagaa atgataaaag accaatatat
     4921 gaccagcttg ttgagatatt aagaactaag attgaaaatg agatggaacc aaacgataga
     4981 atgctttccg aaagaaaaat ttgtgatgaa tatggagtaa gtagaaccac agttagacta
     5041 gctatggctg agttggaaca tatgggatat atatacaaaa gacatggaaa aggaactttt
     5101 gttgctgcac taagtcaaaa ttctcaaaac ttaatggaaa gttatagttt tacagattat
     5161 atgagaacac aaggaaaaaa gccaggtaca aaggtattat catttgaggt agtagaaagt
     5221 acaaagtatt tctctgaaaa attaggtata actactggtg aaaaaatgat aaaaataatg
     5281 cgtttaagac ttgctgatga ccttcctatg atgcttgaaa gaacatatct tcctatgaaa
     5341 gaatttatag gattgacaaa agaaaaaatc gaacaaaaac ctttatatga aatatttaga
     5401 gaagattatg gcgagattat taaagttgct gatgaagaat tttcagctgg aataatctct
     5461 gataaggaag ctaagttatt ggaagtacca ggagattcac cttgtctgaa attacttcgt
     5521 accacatata atgatgacaa tatagttatt gaatttactt tgagtgtcgc tagaagcgat
     5581 aaatttgtat atagagtaag acatgtaaga tagtaagtta aatagctttc aagaaaacag
     5641 aatgatatta taagcgtcaa tgccacaatg atagtataat tataaggagg ttattttgaa
     5701 aagttttatt tatgcagata aattttttat gaaacatgga atcaaagaaa aaggttacct
     5761 tagtataatt aatggcaaat tcggtgaatt tcaacaagaa agacctaata atactgataa
     5821 tgttatagat ttttcaggta aatatattgc tcctggctta gttgatactc acattcacgg
     5881 tcttttaggt gcagatgtca tggacaatac atttgaagca ataaacacaa tttcaaaagg
     5941 cttattaaaa tatggagtaa catctttcct acctacaact ttaactgatt ctgtagatac
     6001 actaagtgat tctgtagaaa atataagcaa tagcttcaat cgagttgagg gtgcaaaaat
     6061 acaagggatt tatttagaag gtccattttt tacagaaaaa tataagggcg ctcaaaatga
     6121 aaaatatttt aaagaccctg acatcaatat attaaaatta tggcaaaaaa aatcgaaaaa
     6181 tttaataagg aaaatagcaa ttgctccaga acgtaaaggt tcaatagaat taacaagata
     6241 tgcaactaaa aataatatat cagtttctct tggtcatagt tcagctacat ttgagcaagt
     6301 taaacaagtc gttaattcag gtgctaaagt gtttgtacat acatataatg ggatgagtcc
     6361 tttaaatcat agagaacctg gaatggttgg ggctgctatg gtactaaaag atacttatgc
     6421 tgaattaatt tgtgatggtc atcatgtaag cccaatagct gttaaaataa tgatggatgc
     6481 taagtctagt aataacatag ctttaattac agattgtatg agagctggtg ctatggagga
     6541 tggaaaatat actcttggta agtttaatgt aaatgtaaaa aatagtgttg caagattaaa
     6601 tagtgggagt ttagctggaa gtgtattaac tatggacaag gctgtgagaa atatagttga
     6661 ttggaacata tcaactttag aagatgcaat aaaaatggct acatatatcc ctgctttaag
     6721 ttgtaatatt gataatgtct gtggctctat atatcaaggt atggatgcag actttatagt
     6781 aacagataat gactttaata tacacgaaac ttatataaat ggtatctgta tgtacaaagt
     6841 taataaatag attcaatata aagttataga ggcttcctaa aaatagagat aagcctcttt
     6901 ttcattgtta aaatgatgaa aatacatttg atttcataca aaaagagtgc ctcatgaact
     6961 aaaaagttca ttttgagaca tctcttttat aatgttcttt attttatagt ataagtttat
     7021 aaaaattaaa tcctatttat caatctgttc tctaagccac ttagttgcta catctaactg
     7081 tttattttca tctttttctt caatagtagg ctttatacct ttttcattga tataatctcc
     7141 attaggtgta aaatattgag cagtagttaa cttgtatcct gttccatcct tcaatcttac
     7201 aactgattgt accaaccctt taccaaaagt tgtagtccct acagaaatac cttctttatt
     7261 atcaacgatt gcagctgtta atatttcagc agcagaagca ctttctccat tagttaacac
     7321 tactattgga agtcccaatt tttctttatc agatttcaag tactcagtat ttcccttatt
     7381 atcttttgta tatactatgg ttccttcacc aattaactca tcagcaactt ctttacatat
     7441 atctagcatt ccaccaggat tatttctaag gtctattact aatcccttga tattttttgc
     7501 ttttaattct tttaaagctt tatcaaagtc agtatatgta cttgatataa attgggtaat
     7561 ttctatatat cctaaatcat tatcaattac tttactttct atactcttct caattatctg
     7621 gtctgttttt actttaaagt tcaattgttt atcttctctt aatatagtca gttcaactgt
     7681 tttacctttt ttacccttca tcatacgaac agcttcatct gaatttttat atgagacaga
     7741 ttttccattg acttttacta atttatcccc tgattttaca cctgctttct ctgctggaga
     7801 atctttcatg ggaactacta ctaagccacc atcttttgat gcccctatat acatacctac
     7861 accaacataa gaaccttcac tatcattaat tagattctcc atttcatctt tagtataata
     7921 accagaatac ttatcatttg ttcctagaaa tagacctttt gctgctccat ttaccaaatc
     7981 agtatctgat acttttttat aaaaatcttg ttttataata gattctagtc ctaacaactt
     8041 atcatatttt ttatagtctt catagagttc tttcgatatt acaactttat tccccaatgt
     8101 caactgaaat gctgatgtaa ccatagctgt tataattact aaaatcaccc ctaaaaaaat
     8161 agctttcttc tttgaaatca caagatacat ctccttttac ataaactatt attattatat
     8221 gactagctgt tatttttatt acatttaccc aaatatattg ctttattaca ataaattata
     8281 ctaaaattta tattaaatac aattgctgta cacattataa catattaagg gaggaattat
     8341 tttatgaaaa aagaatttag aaaaaaagtt attgaatcaa gaaaaaataa gagtgcaaac
     8401 tttgtactta gtaattcaaa tttaataact gaaaaattac ttcaaatgga agatataaga
     8461 aatgcaacta atataatgct ttatctaaat tttaacaatg aagttcaaac agataattta
     8521 atcaaaaaac ttttgtctct aaagaaaata gtatcaagtc ccatcacaat aaaagaaaat
     8581 catacattaa tccctactca aataacagat ttaaaagagg gattgagaat tggagcatat
     8641 ggaataagag aacctaacga aaaatcacct acaattgata ttaaggcttt agatgttgta
     8701 attgtaccag ctgtagcata tgatatacat tgctatagat taggatatgg tggaggattt
     8761 tatgatagat ttttagaaaa cttaagaaaa gatgctataa ctatcggcat agcatttgac
     8821 ttccagatat tcgattctat tccaaaagaa actcatgatg cacaattaga ctacatagtt
     8881 actgaaacta gaattatcac tccaaatagt tagaatatta agtatttcca gaccccattt
     8941 aatgtagtaa taaatactac tgttaatatc ttactttata agcattttaa gtcatggctt
     9001 ttacaacaat aagaaccata cacaatttac ttacattaat aaaaaccatg actttattgt
     9061 acaatatgca gttatttaat tataaaatac cttcttattt cgtcgcattt aattctattc
     9121 tatctggaag ttcactcata tttgtttcaa caactatgta tggaaaagaa tcttccttat
     9181 ctttatactt tatcttgact ctcaaaacat cctttccttc ctcaaccaca ctttctattt
     9241 tgtccatttc tataccatat ttattatttc ctctagtcac tataatgtat attttgttat
     9301 cgattttagc cgctaatgct ctttcttcat ttgtatactt atccatcaca tctaatatct
     9361 tttctggtat tgaatttctt tgtaatactg tataatctat gggtttagat ttctctacaa
     9421 acatacttgg tatgaatata aatcccccta taacgaatac aagagctata actccataaa
     9481 ttataccttt aatattaaac tttacattca atcttatcac tcctcccata tattctaaat
     9541 atatgattta taaacttatc ttagactcta aattattgat tagtatatat ttatacataa
     9601 ttataaatat atgtaatgta actaaaaaac atgttttttc tatttcataa taaacgagct
     9661 aaaaaataaa aagagctata tgaaaacatt ttcattgttt tcatatagct cttgagttta
     9721 caatattaaa aaatacttat ttgtcactga ttccttcttg atatccagat gtttttgtaa
     9781 tctgtctata aagaacacaa accagtatta tgacgcccat atatccagta tatggatata
     9841 aaataccaac cagcttatca aatggtagga agccacctat aagacctaat atagctgcta
     9901 ttaaagttat taatttaaac ttaggatgtt tatcatctac aaatctatta gttactgacc
     9961 ataataaagg tacagctgtt gtatatatac ctaatataag aattattgag aataaaactc
    10021 caattactgg tgaaatctta tctgctaaga ataatgatgg tattgctttt gaatataagt
    10081 ttcctatatc agaaagaata gctaagtgca tcattatagc tgcagccata agtgctactc
    10141 cacctaatat accaccacaa atagcttctt ttttgttagc agcacttgct cctaatccag
    10201 ttaagaatgc cataacaaca attgtgttat aagtagtata tattacacca gatagatagg
    10261 catttggaac tggtttagct atctttacag tgctaagaac ttgtgcagct tctcctatat
    10321 tacctatgtt tttagctaaa cttataccac caactaaaag tgtaaagatt attattatag
    10381 gccctattcc acctagtatc tttgataatt tatcaagacc tagtgaaaca gttaataatg
    10441 ctactactgc catacctact ctacctacaa atggatttaa tccatagtat tcagttaaag
    10501 ttgctcctgc tcctgaaatc attactacga aaacaccaaa taagaataaa ggtccaaacc
    10561 attcaaagaa tattcctaaa tacttaccac aataaacacg ataaattttg ataggttctt
    10621 taagttgaag ctctttacct tttgacataa ctgaagcacc catccatgag aataatacca
    10681 ttgaaattat cccaccaata atacctgcaa aaccataaaa agagaagaat tgcattattt
    10741 cctgtcctgt tgcaaatcca gaaccgatta ctgttgctac atacgcacct gcgaaactga
    10801 tgactgtttt aacatttact ttttctgaca tatctcagtt acctccccat gtgaaatttt
    10861 ttcttctgat taaacaattc gataaactac ctacaatttc ctttaacata aaatacatga
    10921 taactgtttt atagcttttt agtataataa cttaaaactt ataatctgat tttataccct
    10981 actcctcctc tcataatttt ttttagattc ttttatctaa gtagtcatta tatataaaat
    11041 tagtttctct ataaat
//
LOCUS       DAEOSP010000130        11019 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.132, whole genome shotgun sequence.
ACCESSION   DAEOSP010000130 DAEOSP010000000
VERSION     DAEOSP010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11019
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.132"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(63..695)
                     /locus_tag="KRH54_002559"
     CDS             complement(63..695)
                     /locus_tag="KRH54_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG8580209.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(767..1120)
                     /locus_tag="KRH54_002560"
     CDS             complement(767..1120)
                     /locus_tag="KRH54_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBG8580210.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKAQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNVLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(1198..1401)
                     /locus_tag="KRH54_002561"
                     /pseudo
     CDS             complement(1198..1401)
                     /locus_tag="KRH54_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
     gene            complement(1791..2021)
                     /locus_tag="KRH54_002562"
     CDS             complement(1791..2021)
                     /locus_tag="KRH54_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8580211.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(2023..2430)
                     /locus_tag="KRH54_002563"
     CDS             complement(2023..2430)
                     /locus_tag="KRH54_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG8580212.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            complement(2791..3229)
                     /locus_tag="KRH54_002564"
                     /pseudo
     CDS             complement(2791..3229)
                     /locus_tag="KRH54_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3243..4169)
                     /locus_tag="KRH54_002565"
     CDS             complement(3243..4169)
                     /locus_tag="KRH54_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580213.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(4294..5076)
                     /locus_tag="KRH54_002566"
     CDS             complement(4294..5076)
                     /locus_tag="KRH54_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBG8580214.1"
                     /translation="MTKTQQEMIQNVLEFSKRDSRIIGLAISGSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYTCTKIGRR
                     ELFETIDFLSFLRQSVLASLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(5232..5852)
                     /locus_tag="KRH54_002567"
     CDS             complement(5232..5852)
                     /locus_tag="KRH54_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG8580215.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5979..6647)
                     /locus_tag="KRH54_002568"
                     /pseudo
     CDS             complement(5979..6647)
                     /locus_tag="KRH54_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6739..7554
                     /locus_tag="KRH54_002569"
                     /pseudo
     CDS             6739..7554
                     /locus_tag="KRH54_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            8225..8992
                     /locus_tag="KRH54_002570"
     CDS             8225..8992
                     /locus_tag="KRH54_002570"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBG8580216.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(9325..10002)
                     /locus_tag="KRH54_002571"
     CDS             complement(9325..10002)
                     /locus_tag="KRH54_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580217.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(10152..10469)
                     /locus_tag="KRH54_002572"
     CDS             complement(10152..10469)
                     /locus_tag="KRH54_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8580218.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(10491..10829)
                     /locus_tag="KRH54_002573"
     CDS             complement(10491..10829)
                     /locus_tag="KRH54_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8580219.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         3673 a         1725 c         1560 g         4061 t
ORIGIN      
        1 ttgaaaaagc tcttataaaa tgattattaa agtaaagttt aatataaata aattataaaa
       61 tattaaacta atttatcaat atttcctgat tgaatatgct taatgtttga ttgaattttt
      121 ttatatggcc aatcccacca tttaatttct aataattttg aaattgtatc attattaaat
      181 ctctttttta taatttttgc aggtactcct ccaacaatag aatacggtgg aacgtcatta
      241 gtaactactg ctcttgttcc aataattgca ccatcaccaa tatttacacc tgacataatt
      301 atagaatcat aacctatcca cacatcattt ccaattataa tgttaccctt attgtcccaa
      361 gcgtccgtta tatggttgat atcgagtttc cattcttcat aaaagatggg aaaagtatat
      421 gtggattttg atttcattga gtgatttcca ctagtcatta gaaattttgc attacatgct
      481 attgagcaaa acttaccaat tattaattta tcattgttta tagggtaatg atacaataca
      541 ttgtttttct caaaatctct tgggtcatta taaaagtcat tatatattgt gtaatctcca
      601 acttctatat tttcccttgt tattacattt ttaagatata cagtttgata atcattactt
      661 cttggatata ccttatttga acttggaata gtcatattta aatctctcct ttaatatata
      721 ttatgttttt gactattcca ctactacaat acatctcttc acagaatcac tccttgttaa
      781 gaatcttcat aagataatgg cgtccttttt tagtgttaag tcggatattc tttcttagta
      841 cattcttttt agcgtataac tgacaaactg tcattttctt accaatagtg tcaatgctat
      901 catcaaggtc attttgtatt gctttttcct tatcccttaa tgatatatca tcacgttttc
      961 cctttggggg tttatcagta ggtacaagtt tccaagagta aacaaattgt gctttaccaa
     1021 atgaatatac atacttatat gcgtatcttc catcttttct ctggctctct ccagttctta
     1081 aaattctatc tttattatct cgtctttttt cagttgacat gataagaagc tcctctcaat
     1141 aaccgaattg agcttgatac gtcatatgaa tattataaca tataaggctc aatttcatta
     1201 gataacgtca agtgtatcaa cgattttctc aaattgtttt ctctttattt gaatgcgatt
     1261 gctattcata atgacccagc cagaatcaag gttttcatca gcaagtttac gtaatttttt
     1321 ctctccaata cgaaagtatt ttgacgctta ttcaatcgtc aaggtatatt tttcccaaat
     1381 aggtacatca gtattgttca tagaatgtat cactcctttc tatgttcgta gttcagaagt
     1441 agatagatgg ctttggtaag ctccattggt atttcaacca atcccattct tatgggcaga
     1501 accgtgtcat acaggtgtat cattatactg tggtacaggt tacggcaaaa caaccgttcc
     1561 aaaggttatt cacaaatcac tatatagtag ctctcttgat taaggtcatg gcgtatagta
     1621 tttgttggct atctgtatca caaacgtatc tatctacttt attcagttgt caaagaacag
     1681 tcggcattga tttgcctata aaaaatcaca atagcttatg ggcttttata gacaaataat
     1741 tagtgattct ccatgtacca aatagcttga tacatggaga gagggcgaga ttaagacaaa
     1801 ttaaatctca aaatagcagt gattagtttt gttcctagca ttcgctttgt ttcttcttct
     1861 acatgaatat agggatttcc gtattcatca aataaagttt tctgtgaaag acgaatgata
     1921 tagtgttcaa aatgtttaag tattacgttt attgcattta catcgccatg tacagcaagt
     1981 aaaataattg taaaaggtaa tacttcttta gtaggtttca tttcaatgtt cctgcatgag
     2041 ttctttcaac tcttttagtg catttgtttt gtgataatgt atggtatttt ctgctaaatc
     2101 catcaatgtt gctatgtcag catttgtcat gttgagaaag aatgaaagta gaattatatc
     2161 ttgttttctt tttgtaagtt ttccgattgc ttgtgccatt aatacatctt caattcgtat
     2221 gttgtagtcg taagaaacca tacagatgta gttagaatca taatcatcta aaatactcaa
     2281 tttacttaat tctgcaagtg acaaagattc gagagatacc aaataatcat ttctgcgttt
     2341 attttcgtca taaatatctc tagcatagtt acgcaaaact gttttacaga aactgtcgaa
     2401 ttgatgttca attgtctttt catcaaccat aagatttcac cctctttcca tcgcaaggag
     2461 ttaaataacc ttgcttactc ctatcacggt gggagggcgt taaatcttcg ataaatttca
     2521 aaaaaaaaga gaaaaaaata cacactctgc ctatagggca aaaatgtgta ttctttaaaa
     2581 cgtatttaat ttatattggt tgtaagccac caccatattg atgtatggaa atatgcattg
     2641 gctagctttt cataaaaata caaagatgca taagaaatta atagagggga aaaacttatt
     2701 ctgttattaa aaagtaggtt aattctaata aatgctattg cggtcgaatt aaactatata
     2761 catggcaatc aatcatgttt ctattcttta ttatgaattg tgacagtatt cttcaatctt
     2821 aaagccacat tttacttgac tctctattgt aggaatatac attagcatga attttgttaa
     2881 ggttgtattt ttcaaaggca attttgcata tttcttttac tgcatttgtt gctatccctt
     2941 tgccccaata gttcctagca agtcagtaac ctaattcagc atttttatta atagaggtat
     3001 cttcttcaaa tgttaggctg atacttccaa tagccctttc ttgataaaaa atagcatagc
     3061 taagttgttt aacatctgaa gttgtcaaac aaaaactaat aaaatctcta gcgttttgct
     3121 acctataagg gtgggggaag ttgtcacgaa gattgtttgc aatttcttta tcattcgcaa
     3181 actcaactaa atcggtaata taagcataat tccactctat aagttccatt tttcttctcc
     3241 ttttatgaaa cagctacaag aaatctttct aagccaatac cacttatcaa acagcgttct
     3301 tttttatttc caagcatttg ataagtccag tcaacaaatc caccgtcaac tatttcaacg
     3361 gtttcatttc cttgtttgac cgataatttg aaattgatac ctttataata attattatca
     3421 acatcatcat tatgaattga taactctata ttaggaaaaa taagtttgat agtttcaacc
     3481 attctgtcga aaaatccgtc attatctttg tatccgtttc ttttgcgtag aataatagag
     3541 aaacgaacat tttctatttc acttaatata tctttataat agtttaagtg cttttctaac
     3601 aacatttttt cacaagtgta agaccctgta tctataccag atgaaaccat acaatataag
     3661 ccaaaatgtg aatataaacc tttacctaca aaattttgtg ctcttattac cctagcagta
     3721 gtagccatgt ggataggtat ggtattattt tcttcttttc tttttaattt atcagcaata
     3781 attattgcca acatatttga ggggtctgca agtgtttctg tccctcgtag agcacttact
     3841 acattatatt gatctacgga gccaaaaacg ctacagctac ctaatggagc agagggagaa
     3901 agcataatcg tcttgatatc catattgaat gcttttctca ataattttga ctccaaagca
     3961 tgaatttctt ctggatctat accgctaggg atagtaaatc tatttagttg gtatgacttt
     4021 agaatatcag ttgcagttaa agtatttgct tgtctatcaa acacttctaa tagaagtgag
     4081 ttcaaatctg attttgatag agctgataac ttatcaacta tattcttatc cccaatttta
     4141 tttgtaattc tacttaaaat atcactcata gttatcctcc atactgttac tttcaaaata
     4201 attatagaat tgttacaaca actaatctag gtcatttgac caaaaatgaa aaaacactga
     4261 atatttacta ttcaatattt ttttactttg ggtttatttg attaaatctt gaaaatactg
     4321 taatgcaacc ctttcagctt caacctttcg gacaatagga ctttgtgata attcctctct
     4381 taattcaaga tacaggttga tagtagcctg caatgcatta acacagcttt tatattcatg
     4441 gttagcaacc gtcttttcta atgcttcaat atattggggt gcgtccaatt caatttttct
     4501 aactccacga ggtaatttac ctaagagaag ctgtattaac gaggcaagta ctgattgtct
     4561 taaaaaagat aaaaaatcaa ttgtttcaaa tagttctcta cgtccaatct tagtgcaagt
     4621 gtaatgaacc caaatccaaa atctatcttc aatccactgt aagtctggag ttgggaagta
     4681 tgcttctttt ttagaataaa tttctgttat gcagttgttt tcttcataga gaatagctga
     4741 atcttcaacc ctatctagta aaccatctaa ggttgtaaac ttaaaatcaa catgaagaac
     4801 aggggaatca tagagggaaa ttatcaatcg aggttctcca acatgttcgc cagtaaaagc
     4861 agaaactaat gttccaaact gttccacaat aagtaatctc tcttcgataa cttcttcata
     4921 ggatttatca tctgctacaa tcagaaaatc caaatcagaa tattcatcta acgattttgt
     4981 gatataagaa ccgctaattg caagtcctat tatcctacta tctctttttg aaaattctaa
     5041 tacgttttgt atcatttcct gttgtgtttt agtcataata taacacctct cactgtcttt
     5101 tattagctat caatccaaca taattatgca taggagtatt atataatgaa gaaataaaaa
     5161 aaaataggtc acttgacccg aaataaaaaa gtattgaata attattatcc aatacttttt
     5221 tgaaattata cttatgttgt aggaagattc tttaatacct ctcgctgatt aattacactg
     5281 aatcttttgt tttcataagc aattagatgt tcttgtacac attcatttat agcacgatta
     5341 atgcttctaa tcggagcgtt tacttcgcta gcaagctgtt ttttggtaag cagatttaat
     5401 tcacttctat aatagtgtaa ggatatacac ctcagaagtc gttctttaac acttaaagat
     5461 gataattctt caattaattc agcatttaca actaatttac tagatatttc tcgaatcaga
     5521 aattttgtag cttcaaaatc tccccttaac cagtttaaaa aatcatctct atgaagttta
     5581 tatacattac aatctgtgaa agcgacaatt tcaacgggtt ttttcccatc attaaattgt
     5641 tccatttctc caaaaaaact accacttcta taaagatgaa tagttgagat gtttcctttc
     5701 acatttaaga tgaatgcttc tgcaataccg tcaatcataa gaattacata ttcattttct
     5761 tgctcagcaa aaagaatagt ctctttcttt ttatacgttt ttttaacaag cttcttttta
     5821 atatcttcat tcataaaatc aattaaatgc atattcatct ctccttacca aatctttgat
     5881 taaattatat catctttcaa ttagttaggt aagatagtaa aaatatattt acatagaaac
     5941 tattccatta ttattttcca gttgtcattt ttctgcaaaa acttatcact gtcacgactg
     6001 gagataccag taaggaatgt taggatatca atcgctaaac tttcagaatc ctttttacgg
     6061 ctcaaaatga cgtaatggtc aagcaatcct ttgttttcat catcactggt taaattgaca
     6121 atgtttatct catgggctat ttctggtagc gtttctttcc atttgccgcg ttttgcttta
     6181 gggtcaacta tgaccgcttg tccgccgaat agtaccgcat agtaaacaag catattgtta
     6241 ctgaataatt taccaccacc caatgaaccg ataaacgcag acgctaacgc attggtaata
     6301 gaacccttta ccccttgact agcaagggca ggttttagat agacattctt tccagtgtcc
     6361 agattgtagc caagatagat atcctcatgt tcgccaaggg tttgtgtcgc tccaaaacca
     6421 agtccagcca aaaaatccga agtgacatat tgaatataat cattgatgta acgtttgctg
     6481 acaggaataa attcgccatg caatcccaac atatccccaa agggacgcac gagcttgatt
     6541 gataagtcgt cgtaaaagtc tttgacttca tcacaacgcc tttttagctc gtcaagatcc
     6601 tttgtagata cacggacaac atagctaagt ttatacatag attctttaca gttaaaagtc
     6661 tctttttaaa aaaatgtata aattataaat caaaatatag atactatcaa tgacccacta
     6721 ggtgcaaaaa gcattgatat ggttaaatta tcagacgcta aaagctgggc tatccgaatg
     6781 agtgaagatg ggtgtgctta taaatccatt gacaattata agcgctcact gaaagcgtcg
     6841 tttttcatgg caattcaaga cgactgtatt agaaagaatc catttaattt ttagttaagt
     6901 gatgttttgg aagatacaac agaaccaaaa atcgctctaa caccagaaca ggaaatgggc
     6961 ttattatcct ttatggggag cgataagact tattataaat attatgatga tgttgttatc
     7021 ctcttagaaa caggactacg catttctgaa ttatgcggtt tgactttaga attagatatg
     7081 aaaaataaag ccattatggt agaccatcaa ttgttgagtg atactaagat tggctattat
     7141 gtagaagttc caaagaccac acagggtaag cgagaaattc caatgactga aagagcttat
     7201 caatcatttg aacgtatcgt tgaacggcgt aagaacgcta aagcagaacc aatcattgtt
     7261 gatggttacc agaacttcct atttcttaaa caagatggat taccaaaaca agcaactcat
     7321 tatcaaagca tgttaagagc gttagtcaag aagtataaca agacccatga ggacaagcta
     7381 ccaaagatta cgccacatac ctttaggcat acactctgta caaatatggc taacaaagga
     7441 atgagtacta atatattgca atatattatg gggcataaaa acattactat gacgcttggc
     7501 tattatgcac atggttcatt tagttctgcg agagctgaaa tggaacggtt ataatcctag
     7561 tagtatgact agagtttact acttatttac tacttttgat ggcattctta tacggttaaa
     7621 tgccaactta tgcaaagtat cttccaaaaa gaaaatgtcg atactccttg tagatacagg
     7681 atatatcgac atttaccaac ttatgaaaag ataatcaaat ttaatataag tttttcaaat
     7741 aaaaagatac taaattcttt ttagagaatc tatataacat tacttaagct tggttggggt
     7801 ataaacaatt gagtagctac ttttgtatgg tgttcaactt tagtcaaaac tcttaaataa
     7861 tgcaaatgat ttagattcat taatattcaa tctcttcata agtagtgaaa ttcgagttat
     7921 ttctgcataa ttatactatg atttatagat taattctatt agcatccaga ataatattgt
     7981 ttatatgagt aaaatttaaa gaatattgtg aaaattatag aatgttaaaa taaatttagg
     8041 taaaaaaaag tgtttgtata ttatagagta ccttttctaa aatatgtggg attgttttaa
     8101 gaattagaat aaaaattatt tttaaattag tatttttata atgtatagta tttttaatgt
     8161 ataattaaat tgtgcagaaa atagaattta aagtcctata gaatttctga ttatggagga
     8221 gaatatgaaa agaaaagttc aagtaaaaaa tattactatt ggagaaggta gacctaaaat
     8281 ttgtgtacca attattggga aaaataaaaa agatattata aaagaggcta aagagttaaa
     8341 agatgcatgt ttggatatta tagagtggcg agttgatttt tttgagaatg tagaaaatat
     8401 taaagaagtt aaggaagttc tctatgaatt aagaagttat atacatgata tccctttatt
     8461 attcacattt agaagtgtag tagaaggggg agaaaaatta atatcaagag attattatac
     8521 tactttgaat aaagaaattt ctaatacagg tttagtagat ttaatagatg tagagttatt
     8581 tatgggagat gaggtcatag atgaagtagt taattttgct cataaaaaag aagtgaaagt
     8641 aattatatct aatcatgatt ttaataaaac tccaaaaaaa gaagagattg tatctcgttt
     8701 atgtagaatg caggaattgg gcgcagattt acccaaaata gctgttatgc cgcaaaatga
     8761 aaaagatgtg ttagtgctac ttgaagctac aaatgaaatg tttaaaattt atgcagatag
     8821 accaataata acaatgtcaa tgtcaggaat gggtgttata agtagattgt gtggtgaaat
     8881 atttggttct gcattgactt ttggagctgc aaaaagtgtt tcagcaccag gacaaatatc
     8941 atttaaagag ttaaattcag tacttaatct attacataag agtattaatt aaattaatta
     9001 tatagaaata ttagaaggta ttttattttt gaagatataa attaaatata aaaaactttt
     9061 cagatagtaa aatttaaatt gggcaattat atctgaaaag caactattta gtgtgtattt
     9121 tgaaagtgat atagaaaatc ttataaatca actatggcat atttaaaata tgccattaaa
     9181 acctactaga cattagatta tagatggtat actagcattt ctattattta tagatagtaa
     9241 gttttcataa taactgtttt atctaaagta gaagatatat ttgtattatg tatatttgtc
     9301 attatttttt tgtaattatg tatattaatg gttatatatg taatataata ttatcactct
     9361 attttttgta tattgtacat tacaacattt atatctatca tcatcaatta cagtagaatg
     9421 tatgggatac ttattttcac ttaaccaata tttttgcata ttctttgtcc aagactttgg
     9481 aaacttcaat acttttttgt ttattataac atcctcatct tcaaaaatat taacttctct
     9541 ttctattttt atttttctgt tgtatatctt attgatagat tctttgtcga gttttatgtc
     9601 cctatacata tcagggcaat agcaacaaac ataatttctt ttttttcgac gtttaaattt
     9661 cacaactttc tctatgctaa tttctaattt ttttgtacca tcttttataa atttaatatc
     9721 ttctatgttc tcgcttgaaa aaatttctgt caatcttaaa gtatctacag tttctctaat
     9781 tatttgcttt ttataagaag agaatccata tccaaaagaa aggtttaatt tattagggat
     9841 gtagccattt ttatatttaa acatcttaat tttttccaat tcagcattat ctttaaattt
     9901 caacttttca tacctaaaag ttactttgct aaaaagagta tattctacat caatatattc
     9961 ttttgcaatc ttaaatttat cgctaccttc aatcacaatc atagcctatc tccaatcgtt
    10021 ttatattaaa ataaggccat agaacatggt ctaatagtac tcattagcac tataagacaa
    10081 tgttaacaca aaaagacaaa aaaaggttat ttatttcaaa taaccttttt ttttatataa
    10141 aatataaaga attatatttt attctttttt atgatgtcta taatctgtga tatcagattt
    10201 agttctcttg aatttaagtc ttttaactct tcatttatat cattttctaa atatgttttt
    10261 ccagtggaag taatatcttt tataagaaca tctgcagaaa tatttaatat atttacaaat
    10321 tttatgaatg ttttaagact tggattcttt ttaccacttt cgatttgagt tatataccaa
    10381 ggcgatactc caaccatatt tgcaaattct tttgaagtta aatttgcttt tttccgttct
    10441 tctttaagtc ttttacctat ataattcatt attgttacta tttccctcac ttatatttct
    10501 ttgtatttaa tgatgcactt tattaactct agcattgcaa tttgttcttt ttcagttaat
    10561 ttattaatca tttcataaca ttctaaaggt acagagttat caacgccttc tgttgtaata
    10621 atatttgcaa ataacaattc aagtggtatg tctaaagcag tagcaatttt cattataata
    10681 ttgatagatg tatttgtctc agctctttca atctgaccaa tatatgttgg atgtaaacct
    10741 gccttttctg ctagaaactc ttgactatat cccaattttg ttctatagac ttttattcta
    10801 tctcctatta tttttcctat atttgccaat tattatccct ccactttaga ttatattcat
    10861 agtttaagtt gttttatata gactatttat acttttttag tctaaatata gactatatat
    10921 gtttttatac tgaaataagt gctatttatt tttgtatagt ctgtatctac aataaacaaa
    10981 tatggataaa tcatattttt aattagagaa atattttca
//
LOCUS       DAEOSP010000131        10898 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.133, whole genome shotgun sequence.
ACCESSION   DAEOSP010000131 DAEOSP010000000
VERSION     DAEOSP010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10898
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.133"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(32..1090)
                     /locus_tag="KRH54_002574"
     CDS             complement(32..1090)
                     /locus_tag="KRH54_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBG8580220.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSKIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            1157..1528
                     /locus_tag="KRH54_002575"
     CDS             1157..1528
                     /locus_tag="KRH54_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBG8580221.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIESRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            1565..2239
                     /locus_tag="KRH54_002576"
     CDS             1565..2239
                     /locus_tag="KRH54_002576"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBG8580222.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            2488..3327
                     /locus_tag="KRH54_002577"
     CDS             2488..3327
                     /locus_tag="KRH54_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBG8580223.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDSNDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            3571..3667
                     /locus_tag="KRH54_002578"
     tRNA            3571..3667
                     /locus_tag="KRH54_002578"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3605..3607,aa:Sec,seq:tca)
     gene            3818..4525
                     /locus_tag="KRH54_002579"
     CDS             3818..4525
                     /locus_tag="KRH54_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBG8580224.1"
                     /translation="MENNFTTRTSFLVGDDGIKKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(4667..5503)
                     /locus_tag="KRH54_002580"
     CDS             complement(4667..5503)
                     /locus_tag="KRH54_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8580225.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSKAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            5691..6596
                     /locus_tag="KRH54_002581"
     CDS             5691..6596
                     /locus_tag="KRH54_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBG8580226.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            6605..7789
                     /locus_tag="KRH54_002582"
     CDS             6605..7789
                     /locus_tag="KRH54_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8580227.1"
                     /translation="MEIRYAKEEEIESIKDIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            7904..9205
                     /locus_tag="KRH54_002583"
     CDS             7904..9205
                     /locus_tag="KRH54_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBG8580228.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTFIESIFFLLEKISNLSFAYINIENPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            9159..10202
                     /gene="holA"
                     /locus_tag="KRH54_002584"
     CDS             9159..10202
                     /gene="holA"
                     /locus_tag="KRH54_002584"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBG8580229.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(10389..10655)
                     /gene="rpsT"
                     /locus_tag="KRH54_002585"
     CDS             complement(10389..10655)
                     /gene="rpsT"
                     /locus_tag="KRH54_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBG8580230.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
BASE COUNT         4266 a         1119 c         1682 g         3831 t
ORIGIN      
        1 tatgtataat agatataatt attatttaaa cttattgttt atttaatttt tcaatagaaa
       61 aagatttatc tgtaagttta aaattatctt ttataccctt agttaagtat actttcttat
      121 ccttagttat gaatatagca tctacattat caattttatt aattaaattc ataccttttt
      181 caagaccaag accaaatgta gaagtggata aggcatcaca attaatagat ttatcagaga
      241 ttattgtaac actacttaaa ttgttttcaa aaggataccc tgtaaatgga tttaacatat
      301 gatgatatat ttttccgtct ttttcgataa atctttcata aattccagaa gtaacaacag
      361 acttatttgt agtttctatg tttcctatgg attcaccatt tttactagtt gggtcttgaa
      421 tacctatttt aaaaggttta tcatttttac cattttctaa agtaaatata tttcctccta
      481 agtttactaa accttttttt acatcctctg attttaaata ggctacaatt ttatcagcag
      541 catatccttt agctatggca cctaaatcaa ttttcatatt ttctttggtt aattttatag
      601 atttattttt ttcatccaat atgatgttct tataatcaat aagaggcaga agtttttgta
      661 tcttagaatc acttggaact ttagctttat ctgttccaat agcccataaa tttgataatg
      721 gaccaattgt tatgtcaaaa tatccatctg ataatttgga atattgtata gcttctttta
      781 cgacgaaaaa tgtatcatca gaaactctga caaatttttt accagcatta tcatttattt
      841 tagaaacatc acttcctggt atatgagtgc tcattttgtt gtcaatatat cttaaaattg
      901 aatcacattc atttaagatt ttatcacttt tggattcttt tacattaaat acagtaactt
      961 cgtttactgt accaaggtag tagctagttt tactatattt agtattctgg cttgaactcc
     1021 taaaaaatga aaataaaatt aatgctaaaa tcaatatcac aaaaataaat gtgatttttt
     1081 tcttattcat ggtatattcc tcctatgaaa ttgtgttatt attttattat aacataattt
     1141 cataggagga caaaaaatga agaaaaaaga cattcttctt attttaggca ttttggttgt
     1201 tattacagca tgttatggga taataaatgt tataaattct aaaaatgctg gaaatattga
     1261 gatttatgta gataataaat tgtataaaac tgtttcaata aatgccaaag aagaatttaa
     1321 aatagagagt agaggggggt ataacatagt aaagattcat gataaagggg ttgagatagt
     1381 agatgcttca tgccctgata aggtttgcgt acatactgga tttataaaca agccaagtca
     1441 aagcatagtt tgtataccta atagagtgag cataaaaata aatacaaatg aaaagaatga
     1501 taatcaagaa gatataattt ctaaataaaa taataaaaat aattgaaagg aattggtgat
     1561 aattatggtt aacttaggaa acgattggga tgaattattg aaagaagaat ttgaaaaaga
     1621 ttactactta aacttaagaa aatttcttat agaagaatat aaaacacgcc aaattttccc
     1681 aaatatgcac aatatatatg aagctttaaa acacacttca tacaaagaca caaaagtctt
     1741 aatattagga caagaccctt atcatggaga taatcaagct catggtttag ctttttcagt
     1801 tcaaccacaa gtgaaaactc caccatcttt attaaatatg tataaagaat taaaagatga
     1861 tttaggttgt tttataccta ataatggata tttaatgcct tgggcagacc aaggagtgtt
     1921 acttttaaat actgctctta ctgttagagc gcatgaagct aattctcata agaacaaagg
     1981 atgggaaatt ttcactgata gagtaatatc tattttaagt gagcgtgaag acccagttat
     2041 ttttgtacta tggggaagta atgcacgtaa aaaagtagag ttgattgata caagtaaaca
     2101 ttatattttg gaagcacctc atccaagccc attgtcagca agtaaaggct tttttggatg
     2161 taaacacttc tctaagataa atgaaatttt gaaaaagcta ggaaaagaac ctattaattg
     2221 gcagatagaa aatatataaa agtaaaaggt aacaaaatga taacaaaaca acaataaaca
     2281 atgacattgt tgtaactaat agaaatatgc ttaaatagtt gaaaatacta tgtatgcata
     2341 tttctatttt tttgtcaagt tgacaactct tgaaaaaata gttacaattt agttacacaa
     2401 aaaacacaaa taaaaaaaga aacttacaaa gcagaatttt atgaagcaaa atatatagtg
     2461 taaaaaatct tagggaggaa attttatatg aattttaaaa aattgagtaa tgctaaagtc
     2521 tcggtaatgg cagttgtttt atcagttgga atattatcgg gatactctgc gcttaataaa
     2581 caagtaacat tagtagtaag gggagaagaa agaactatat caacatttag ttctaatgta
     2641 ggagaccttt tagaatctca aaacattagc tatgattcaa atgatatagt aactttagat
     2701 gaaaatacaa agttatctaa tggagataaa atagaagtaa ttgatgtaaa ggaaaaaaca
     2761 gttgtagaaa ctaaagacat tccatttgaa gttacagttg ttgaagatgg ctctttatta
     2821 aagggtgatt ctaaggttaa agaagaagga aagtctggta aaagtgaatt aatatataaa
     2881 gaaacttatc acaatggaaa gaaagtagaa aaggaatttg tgaaaaaggt tgttaaagct
     2941 gagccagtaa ataaggttgt aagcaaaggg acaaaggtag aaatgcaagt agcctcatca
     3001 aggggagaaa gttcaagaag aatggcttca tcatcatcta gtagcagtag tagtaacaat
     3061 ggtaaaaata tgagagtggt agcaactgca tatactggtc acagtataac tgcaacagga
     3121 acaaaaccta agtggggaac aatagctgta gaccctaagg ttataccata tggaactaaa
     3181 gtttatatac ctcaatttga catggtgttt actgcagaag actgtggtgg agcaataaaa
     3241 ggaaataaaa ttgatatcta catgaatgat tcaaaaacag tttataactg gggaagaaaa
     3301 actatagata tacgtatatt aaaatagtta aataatttat atttttactg gaaattatat
     3361 tttgattata tagagaagaa actctttata gattctaaaa atataatttc cagtttttta
     3421 ttttggatat gtaataaaac aggtaaattt taaccatgta tagataaaag ctatttaaat
     3481 tataatttac tatcatataa atctataaaa attgttgcaa agagtcaaaa tatatgatat
     3541 tatagatttc gttgagaagg atttcattat gggagtggat gggtgccggt gtgccctcta
     3601 gtcttcaaaa ctagtatgag gggttaagag cctcttaggt gggttcgatt cccacgcatt
     3661 cccgccaata tgtaaaaggt atcttttaat taaaagatac ctttttaaat ttattttaag
     3721 ataatttatt tttaataata tataatgttt tgtattatat aatgtaaatt ttctaatact
     3781 attatatatt tagataaaaa tctaggagtg tggaaagatg gaaaataatt ttacaacgag
     3841 aactagtttt ctagttggag atgatgggat aaaaaaactt aataattcaa atataatagt
     3901 atttggagtt ggaggagttg gtagttttac tgttgaagct ttagcaagag ctggagtagg
     3961 caatattact attgtagatt ttgatgatgt agatataacc aatataaata gacaaatacc
     4021 agccctacat tcaactgtag gaagatacaa agttgatgtc atggaagaaa gaatattaga
     4081 tataaatcct aatatcaata taaaaaaaat aagaagtctt tataataaag atactagtga
     4141 cgaaatacta actgaaagat atgattatgt tgtggatgcg atagacatgg taagttccaa
     4201 aatacatcta attgaaactt gtgaaaaaaa aggattgaaa ataataagtt ctatgggtat
     4261 gggaaataaa ttagacccaa ctaaaatagt cgttacagat atacacaaaa caagtacttg
     4321 tcctctagct aaagttatga ggaaagaact tagggataga ggtatcaaaa agttaaaagt
     4381 agtatattct actgaacaac caatagaact taagaagaaa gtaatgaatg gtagaaaagt
     4441 aacacctggt agtgtatcat ttgtcccatc tgtaggaggt ttaataatcg catctgtaat
     4501 agtaaatgaa ctattaggac aataagtttt aaatatttta atttataaat tttatataaa
     4561 aatttgaagg ctagaagcat gaatacaaag caattttata ttcatacttt tagcctaatt
     4621 attttatatt accttatttt atgtagttgt agttttattt tttagattat gtatctattt
     4681 cataattata ttttgtaaat ataatttctc catcactata aaaaacaata tctcctattc
     4741 tatctgttct ataaattgat acatggtttt gttttagatt attaataacc tccctatgtg
     4801 gatgaccata ttgattttta tatccacatg atattgcagc tatatctgga ctaatttttt
     4861 ttataaattc taaagatgaa gaagaattac ttccatgatg acctattttt ataaaattaa
     4921 tattatttaa tttaaaagaa tgtaaaattt ctttttcatt ttcttcttca gcatcaccca
     4981 taaataaaaa agaattatca ttaaaagtta atttgaaaac tatagaattt aaattagatt
     5041 cttcttgtat atatgatgga cttaatacat atatatttat attgttatct atgtttaaaa
     5101 catcattttt atagagatgc tgaatagata agtttttatc cgtacaagaa tttattagat
     5161 tttgataagc cttactatca gttgattgtt caggcatata tatggagttt acattaaatt
     5221 tttttataat attatcaaga cttcctatgt ggtctgaatc tggatgagtt gctattataa
     5281 tatcaatagt ttttattctt ttttgtctaa gataactaat aattatattt tcgctatctt
     5341 catcacctcc atcaattaat atattcttat ttgtgggtgt ttgaactaaa atactatcac
     5401 cttgtccaac atcaatcata tgtatagata gtagagattt tttgtcacag ccgattaaaa
     5461 gtgatattat aataagtatt gataaaaaaa ttttatttct caataaaaaa tcactccatt
     5521 cattacatgt ttatataaat ttatgatggg taatataaga tatatatgac ttattatatt
     5581 ttatatttta aacaatataa atgaaattaa taccataata aaattggagt tttttatata
     5641 tacaaaataa aatttttaaa attaagcaat aaaaaaggta ggaggatttt atgtttttca
     5701 aagatattga actaaattca aaaaaagagt tagacccata ttttgattta gtagattatg
     5761 aagcttgtga gtactgtttt agtactttat atatgtggca acatgtttat aagacaggat
     5821 attatatagg tgaagatttt gccgttttag ttggggaata cgaaggagat agcttctcaa
     5881 tattaccact tgctaaaaaa gataaacttc cagaagttgt agattttgtc cttgaatatt
     5941 tttctaaaaa taataaaaaa atatacttaa gaggtattac tactgaagta gtagagtttt
     6001 taaaagaaaa atatccaggt agatttgagt atatagaaga aagagattta tttgactata
     6061 tttatgatgc agaaagttta agaactctag ctggtaaaaa gaatcaaaag aagagaaacc
     6121 atataaacta tttcttaaaa gagtatgcag gtagatatga agctaaatta ttagataaag
     6181 aaaactttga tgagtgttta gtactaatga aggaatggga aagtaataag gaagaaaata
     6241 atgaatttga tgaaagtatg gatgatgaat taataggtat caaaaagata tttaaccatt
     6301 atgacatatt aaaagataag gtaaaagtgt ttggtgttta tgtagatgga aaattagaag
     6361 cttttagtat aggagagctt ttaaatccaa atatggcatt gattcatata gaaaaagcta
     6421 atcccgatat aagaggtctg tatccattta taaatcaaca atttttggta agtgaattta
     6481 aggacgttga gtttgtaaat agagaagaag atttaggtat tgaaggatta agaaaagcta
     6541 aactttctta tcatccttgc agatttgttg aaaagtacag tgttagggag gcttaataga
     6601 ctatatggag attaggtatg ctaaagaaga agagatagaa agtataaaag atatttggag
     6661 ttattgtttt aacgatacgg agagttttat gaagtactat tttaatgata aatataaatc
     6721 tgaaaatact gtagtagctt tagatgaagg aaaaattatt tcttctcttc agctaaatca
     6781 gtataagtta ttactaaata gcaaggtata taatacatca tatgtggtag gagtttctac
     6841 acttccagag ggaagaggag ctggatatat gaacaaagta atgaaattta ctttaaacga
     6901 attgtataaa aaagggcaat tagtttctat attgatgcct attgactata gactttatag
     6961 aagatttgga tatgagcatt gttacgacca gattgaatat actattaaca cagatgattt
     7021 aaaaaatttt aagtcaagtg gaaaaatgat aaagtctaat ttatcacaaa ttgatgattt
     7081 gatacaaata gatagaactt ttttaaatga ggttaatggt aatgtgttaa aagatgaaca
     7141 ttattatgaa aacttattta aagagattca aagtgaagat ggatttttat acattcatga
     7201 aggaaatgaa aaagatggat atatagttta ttttcttcag gaagataaaa tgtttgtcag
     7261 agagttattt tataaaaata tagatgccct taaatcaatg cttaaattta tatataatca
     7321 caatactcaa tgtaagatag ttactatatc tactcctaca atagataaga taaggtttat
     7381 attagacaac cctaaagata gtgatataaa aataaaacct tttatgatgg gaagagttat
     7441 aaatgtaaag aagtttattg aagatataga tattgagaag gatattaata gctcatttaa
     7501 tttattaata gaagacaagt ttatagatga aaataatggt ctatttaaaa tttctataca
     7561 gaataagaag gtatctgtag agcagttaga taaaaaaggt gcagaaaagc ctcaagaaga
     7621 ttttgatatt aaattagata taaatacctt aacacagtta tcattttctt atatagatgt
     7681 aaatgaagct attttcttaa atgatataaa agatgtttca gaagaaacac tagaaacact
     7741 taattgtatt ttttctaaaa aaaataacta tataaatgaa tatatatagg tagagtacgt
     7801 taaaagattt taaaaatctt ttgacgtatt tttttgttta gataacaatt tatcttgttt
     7861 aaaaattttc tatttgaaaa taatctaaat aatatacttt tatatgggag tgattataat
     7921 tcaaataaga agaccattat taattatatt aatgtttgtg gtagctatat catttatcat
     7981 tacaaataac acaaaaaacg acgaagaact taatgataag ataataacta tacaaggagt
     8041 tgtcaaagga aaaattgaaa agaaaagata caatcagtac aaggtagggg tttttctaat
     8101 aaatgattat acaaaaaata aaaatttgaa gattggtcag aaagttaata ttacaggtaa
     8161 attcaaaagt ttagataaaa tgaagtatga tgattttgat tatggaaggt atataaaaag
     8221 tactggatat aaaggtatag tgtatataaa taactataaa attattgata aaaataaact
     8281 ttactcttta ataggggaaa taaagtttta tattagtaaa acttataggt acttatataa
     8341 gaaaaactca gattttataa attctatact attagcagaa gttgaaaatt tgactgagga
     8401 acaaaaagaa atttttacca gaactggaac tagtcatgta atatctatat caggtcttca
     8461 tacaggaata ttatgtgtaa ttatatcctt tctattaaga ggtataaata aattgtataa
     8521 attattaata ctgtcgattt ttataacttt atattgtata atggtaggtg cgtctccatc
     8581 cataattagg tctatagcat ttgttatgat tttttattta tcaatattta ttgatagaaa
     8641 gaaagatgga atatcggcat tgtctctaat tgggataatt ttaattatga ataaccccta
     8701 tgttatatat aatgtaagtt ttcaattgtc ttttttagct acactttcta tattaatatt
     8761 ctataataaa ataaatagta ttataaaatt aagtatggta tcattgacaa tatcatcaaa
     8821 tatattaact ttacctataa tttattatac atttaaaggt ataccattgc tttcaattat
     8881 aggaaattta attatagtgc catttgtggg agtaataatg tatttaagta ttgcaagttt
     8941 aatcgtattt aaagtaagtg tagtaatagc taaaattata tcttttttta atagtacatt
     9001 tatagaaagc atattttttt tactagaaaa aatcagcaat ttaagttttg catacattaa
     9061 tatagaaaat cctaaatttt atattgttgt tatttattac ataggagtgt ttttttacat
     9121 attttacata gaaggaaaag agataaagga gcaagagaat gaattacaag gatattataa
     9181 ggaatgtaaa agagaaaaac tttgaaaaaa tgtatctttt ttatggaaga gagtattatt
     9241 taattgagaa tgcaataaaa gcatttaaag atagtctaaa cgagggaatg cttgatttta
     9301 atttagatat aatagatggg aaggaaatag tcttaaatca gcttataagc tctatagaaa
     9361 cacttccatt tatggatgat aggaagatag taataataaa agactttgaa ctattaaaag
     9421 gaaagaaaaa gaattttaca gatagtgatg agaaatactt aatagagcat ttagacaata
     9481 ttccagacac cacaactata gtttttgttg tatatggaga tgtagacaaa agaaaatcat
     9541 tagttaagaa aataggaaac aatggaattg tttttgattg tgataagctt tctgatatgg
     9601 atttgtttaa atggataaaa aaatcttttg ctttaaatga tgttataata gataattctc
     9661 aaatcatgta ttttattgaa caagaaggat acagagataa gagtagtgaa aagacattat
     9721 ctgatttaga aaatgaaata aataaaataa gttcatttgt tggtaaaggg aataatgtaa
     9781 ccaatgatgt aattgataaa ttatctcaaa aaaaagttga gaatgatata ttcaaattaa
     9841 tcgattatat tggagaacaa aatgcttcta atgcaatgaa aatattaaat gatatgatac
     9901 aagaaggcga atctgtatta ggtatatttt ctatgatagc aagacaattt aaaataatta
     9961 tgcaagtaag acagttacaa cttgatggat attcaacaaa acttatagct gataaattaa
    10021 aaatgcatca atttgttgta ggaaaagcat taaagcaaac taaaaatttt tctgatgata
    10081 taattgtgga aatattaaac tatatattag aaagtgatta caaaataaag acaggtctta
    10141 taagagatac tttagcagtt gaaatgttgg ttagtagata ctgtaaaaga gaagcaattt
    10201 aaaaaatcta tatattaata aaaaataaat agcaagatat gttatagttc agagtagatg
    10261 taagctaaat taagtttgat atacaatcaa ctataatatg tctaactatt tattttttat
    10321 tgtataaatt tacaaaataa aaaaccccta cataggggtt attatatata agaaatataa
    10381 actggtaatt aagcgttcat accgtttaat ttttgagcta atttagctac ttttctagct
    10441 gcagcattct tatgtatagt tcctttagaa gctacttggt atattctttt ttgagcgtat
    10501 tggaacttag caacagcatc ctctctgttt ccagctgcaa cagcatcttc gaatcttctt
    10561 attgcagttt ttatttgaga ttttctaact ctatttaaag cagttttctt ttcgataaca
    10621 cttattcttt tcttagctga ttttatattt gccaccgtgt tcacctccct gtattttttt
    10681 atcatattca tcgtcaacat attagatttt aacagaaata aatctaaaaa tcaaggatta
    10741 ttatttatat attttaataa tgttttttat tttgattttt tagactgtaa attgtgtata
    10801 aataatttgc aatatcttgt ttttaaattg gtatattttg tgttttaaat attttgacat
    10861 atataattaa tatttcattt tgtaacaaat atttgaga
//
LOCUS       DAEOSP010000132        10761 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.134, whole genome shotgun sequence.
ACCESSION   DAEOSP010000132 DAEOSP010000000
VERSION     DAEOSP010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10761
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.134"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            274..1458
                     /locus_tag="KRH54_002586"
     CDS             274..1458
                     /locus_tag="KRH54_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580231.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(1835..2944)
                     /locus_tag="KRH54_002587"
     CDS             complement(1835..2944)
                     /locus_tag="KRH54_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG8580232.1"
                     /translation="MDFKSLGISENTINILKKLGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDHLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRSLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            3407..4600
                     /gene="nifV"
                     /locus_tag="KRH54_002588"
     CDS             3407..4600
                     /gene="nifV"
                     /locus_tag="KRH54_002588"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBG8580233.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            4625..6550
                     /locus_tag="KRH54_002589"
     CDS             4625..6550
                     /locus_tag="KRH54_002589"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBG8580234.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWVELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            6572..7567
                     /locus_tag="KRH54_002590"
     CDS             6572..7567
                     /locus_tag="KRH54_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBG8580235.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            7893..8366
                     /locus_tag="KRH54_002591"
     CDS             7893..8366
                     /locus_tag="KRH54_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8580236.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            8400..8894
                     /locus_tag="KRH54_002592"
     CDS             8400..8894
                     /locus_tag="KRH54_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8580237.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            8991..9512
                     /locus_tag="KRH54_002593"
     CDS             8991..9512
                     /locus_tag="KRH54_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBG8580238.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYSD
                     SDLDYKKVHFNNF"
     gene            9701..10012
                     /locus_tag="KRH54_002594"
     CDS             9701..10012
                     /locus_tag="KRH54_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBG8580239.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            10040..10726
                     /locus_tag="KRH54_002595"
     CDS             10040..10726
                     /locus_tag="KRH54_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG8580240.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
BASE COUNT         4127 a         1167 c         1948 g         3519 t
ORIGIN      
        1 aataaagaaa aaattatata ataataaaga aaaaatgtat aatatttaac attaaaaaag
       61 atatttcata aaattaaaaa acaaagtgaa tttgaatact atttaaaaaa taaaataaat
      121 atataaaaaa tatttaatat aaaggtagaa ataagttgaa aatgtaaagt cattggaaaa
      181 atatagttga atttaatgag caaaaatgtt attgttttat caataaagtg ttaaaataaa
      241 aacttttaat atcattttag ggaggatgat ttcatgcaaa aaagtgtaaa gaaagcaaaa
      301 gtaacgggct ctatgttagc catttttggt gttgcaagcg ttctttttag ttcacatgca
      361 ggaggaggat ttgctacagg gaatcaagaa actcagtact atgtgcagta tggatggaca
      421 gcaccactta tggcaatttt agcaatgatt atacttactg ctaccatgag agaagttatc
      481 ataatgtata ataataataa ttgtaggaat tataaggatt tattttgtga gctttggaga
      541 ccatatccaa aacttgaaat aatatgggag atatattact atctaatggt tttaatagct
      601 gtaagtgctg ttatagcagg tgcagcagca gtatttcaaa gtataggagt aaattatttt
      661 gtagctgtat ttattattgg agttgtactt cttgtcttta ctatatttgg tgctatgctt
      721 gtatctaaag cagctacagc aatgacaata gctattctag tatgtactct tactattttt
      781 attgttggta taaaagctaa agtaccagaa ataactgaaa tattatcaaa tagaacgagt
      841 ttcacacctg gctatatcaa gccaatactt aatactttta tttatgcagg ttttcaatcg
      901 gttgttatac ctacacttgc aggatgttca agaccacttc ttaagaactc aaaagaagct
      961 actaaggcaa tgataatttc atttgttatg aatgcaattg cattaggact tgctgtaact
     1021 atgctaatgg gatggtatag agaaattatt gctgctgggc aaacaacact ccctactctt
     1081 tatgttgcag gtcaatctgg aaatcatact atatacataa tttataatgt tgcattattc
     1141 ctttgtttaa tgtctacagg agtaactact atatttggtc ttgtaaatag atttgaagac
     1201 cataaagcac tttctttcct ttcttcaaga atgaaaagaa gggtatttac agcttgtgca
     1261 ataatggtag tgtcaatgct tatatcactt acaggattaa gtaatattgt taaatatgga
     1321 tatggatatt gtggttatct tggtctattc actatagtaa ttccattcct tacactagga
     1381 cattataaga ataagaagtt tgcaaaggaa aatccagaag ctaaatggcc agcggaatta
     1441 aatgaaaatg taaattagat gtagtagcaa aaaatattgc taaagagaat actatagata
     1501 agaatactat agataatata tctgcatgtt tattaagatt atttgcaaaa tctaatatta
     1561 aacaactata aacaagcaat ataaaactaa gaataaaaga taaaaagagg gtgtctcaaa
     1621 atggactttt agtttataag gcactctttt ttatatgaag tcaagtatat tttcattatt
     1681 ttgacaataa aagagactaa tcctactttg gatagcctct tttttcttta atataaaata
     1741 gtataacagc aaagtataga ttgatacatg ttttctgtag ctttctatat attacagtgt
     1801 ttgtgtggca ttggtatttt aagcaatcta taaattataa ttctataatt cttctactaa
     1861 ttttagattc tatagccgtc tctatttcac ttaacattcg ttcattttta gggtcaataa
     1921 aagcacatgt atatccttct tcaccagctc taccagttct gccaatacgg tggatgtagt
     1981 cttctggtgt ttctggtaaa tcgtaattat atatatgact tactccagat atatcaagac
     2041 cacgagaagc tacatctgta gctattaagt attgtatatc taaacttcta aatgatttca
     2101 ttattctttc tcgttttgat tgagcaatat cactatgaag tttttgacaa ttatatcctc
     2161 ttatagcaag agcttcttca agatggtcaa cccttctctt agttctacaa aatattattg
     2221 ccataaaagg attatcttca tctagtacct tacataaggc atctaatttt tttctatctg
     2281 tagtttctac tacttcttgt tttatattat taagagtgat ttcttctttt tgaatagaaa
     2341 ctacaacagg attttttgtg tatctataag ctaatttttt tacttgagaa tttatagttg
     2401 cagaaaagca tagtgtttgc tttttgtttg aagtctcttt taaaatagct tccacttcat
     2461 ttttaaatcc cataagcaac atttggtctg cttcatctaa aacaaaagtc ttcaacttac
     2521 taaggtctat tgtttttcta tttagatggt ctaaaagcct acctggtgta gctataatca
     2581 tatgaatatt tccttttagt ttctttattt gagaaccaat atcttttcct ccatatgctg
     2641 caagaatact tacatcttta gattctttaa gtttcatagc ttcttctgtt atttgaatag
     2701 ctagttctct tgtaggagaa agtataagta cttgtatatc atttatatct aatgatatat
     2761 tttcaaatat cggaagtaaa aaagctagtg tttttcctgt tccagtttga gcttctgcta
     2821 ttacatcttt tccttctttt attagtttaa tactttcttt ttgtataggt gtaggtgttg
     2881 ttattcctaa tttttttaga atatttattg tattttcact aatccctaag cttttgaaat
     2941 ccattgttat cttacctttc atgaaaaatt ttctttttta ttaaccaatt aataatatca
     3001 tatttgtaag tatttttagt aaatttttta attattattt atatagctta aaaatatatt
     3061 aagatacttt aaaatggaat gtaacttaaa ttaaatttta gtaggaattt tataggttta
     3121 tttttaaagg ttttttgaaa ataaatctat ttaaaaatta aattaaaatg caagaaaaac
     3181 ataataactt tcatttttaa gaagttttca caaaaaagaa aaaatatagt gcaataaatg
     3241 atacaaagta atataataga ttacaagata tgaaaagcta aaaaaataaa ttgcaaatga
     3301 aattaaaatg aataaaaaat aaaaaaataa tcataagtta gaggtatcaa tgaaaaataa
     3361 gacttaatgt ttttaaaatt aaaagtatac taaggggggc attaatatgt gcgttatatc
     3421 aaaagataga gcaaaggaaa taaaaatagt agatactact ttaagagatg gagagcaaac
     3481 agctggggtt gtatttgcaa atagagaaaa aataatgatt gctgaaatgt taagtgacct
     3541 aggtgttgac caaattgaag ttggtatacc tactatgggt ggagatgaaa aaaatgtaat
     3601 aaagcacata tgtagtagaa atttaaaatc agatataatg gcttggaata gagcagtaat
     3661 aaaagatgtt gaagaatcta tttcttgtgg tgttgatgca gtagctatat caatatcagt
     3721 atctgacatt catatagaaa ataaattgag aacttctaga ggctgggttt tagaaaatat
     3781 ggctaaaaca gttgaatttg caaagaaaaa tggattatat gtttcagtaa atggagaaga
     3841 tgcttcaaga gcagatatag acttcttaac tgaatttata aatgttggaa aacaagcagg
     3901 ggcagataga tttagatact gtgatacagt aggagttatg aatccttttt ctataaagaa
     3961 tgcaatagaa actctttatg aaagaactaa ttttgatata gaaatgcata cacataatga
     4021 ctttgggatg gcaactgcaa atgcacttgc aggaatagca gcaggagcaa attatgttgg
     4081 tgttacagta aatggattag gtgaaagagc aggaaatgca gcattagaag aagttttaat
     4141 ggcacttaag tgtgtttata aatgtgattt aaataatata gatactagaa aatttagagg
     4201 aatatgtgag tatgtagcac aggcatcagg aagaatactt cctacatgga aaccagtagt
     4261 tggggataat atgtttatac atgaatctgg tatacatgca gatggagctt taaaagaccc
     4321 acataactat gaaccatttg acccaagtga agtaaatctt gaaagaaaaa tagtaatagg
     4381 taaacattcg ggaagagctg cagttgtaaa taagttgagt gaatatgaaa tgtatatttc
     4441 tccagaaaac gctaccaagc tattaaatgc tataagagct acatctataa gattaaaaag
     4501 aagtttaatg gataaagaaa ttcttcaatt atattgtgat atactagcac atgagaaagg
     4561 cacaacagaa gaagaagcag ttagaggttc atatatttaa gatataattg tttaggagga
     4621 aaatatggga gataatatag tttataaaat aattaaaaaa catatagtag atggagaagc
     4681 tgttgctgga agttcaattg gtataaaaat agaccaaact ttaacacaag attctactgg
     4741 aacaatgaca tatcttcaac tagaagctat gggaatagat aaagtaaaga ctaaaagaag
     4801 tgtggcattt gtagaccata atatgttaca acaaggattt gaaaatgcag acgaccataa
     4861 gtatatacaa acagtggcag ataaatatgg agtttatttc tctaagccag gaaatggtat
     4921 ttgtcatcaa gtatttttag aaagattttc aacaccagga gatacacttt taggttctga
     4981 tagccacaca cctactgctg gaggtgtggg catgatggct attggagctg gtggattaga
     5041 tgttgcttta gccatggcag gaggagcata ctatataaaa gcaccaaaag tctgtaaggt
     5101 gaatctagtt ggaaaattaa ataatatggt atcatcaaaa gatataatat tagaagtgtt
     5161 aagaaaacaa actgtaaaag gtggagtagg taaagtttat gaatatggtg gagaaggtgt
     5221 caaaagccta tctgttccac aaagagctac aataacaaat atgggggctg aacttggagc
     5281 aactacttca atcttcccaa gtgatgaaaa aacactagaa ttttttaaga gtcaaggaag
     5341 agaagacgca tgggtagagc taaaaccaga tgcagatgca gtttatgatg aagagattac
     5401 tataaacttg gatgaattaa aaccattagc tgcaaaacct catagcccag acaatgtaga
     5461 tgaagttgaa aatataggca agattaaaat agaccaagtg gcaataggaa gttgtacaaa
     5521 ttcttcttat gaagacttaa tgaaagtagc acaaatcctg aaaggaaata aagtacataa
     5581 ggatgtgagt ttggtaatag caccaggttc aagacaagtt atggagatga ttgctagaaa
     5641 tggggctttg gcagatataa taagtgctgg agctagaata ctagaaaatt cttgtggtcc
     5701 atgtattggt atgggtcaat ctccaggtac tgactctgtg tctttaagaa ctttcaatag
     5761 aaatttctat ggtagaagtg ggactttgtc agcacaagta tatttagtaa gtccagaagt
     5821 ggcagcagta agtgcaatca agggagtgct tacagaccca agagaatttg atattaaatt
     5881 tacaaattta gatgtaaatg aatttttgat agatgatagc atgataatta aacctgctga
     5941 tgtaggaagt gatgtagaag tagttagagg acctaatata aaacctttcc ctcttaatac
     6001 agagctaagt cagtctatag gtggaaaagt tatattaaag acagaagata atataactac
     6061 tgaccatata atgccatcaa atgctaagtt acttcctttt agaagtaata taccgtatct
     6121 tgcaaactat tgtttcaata ctgtagatac agaatttcct cagagagcaa aagacaataa
     6181 tggtggattt atagttggtg gagataatta tggtcaaggt tcaagtagag aacatgcagc
     6241 tttagcacca ttatatctag gtgttaaagg ggtaatagtt aagagctttg caagaatcca
     6301 caaggctaac ttgataaata gtggtataat tccaatggag ttttgtgatg agaaagatta
     6361 tgaaaatata tctcttttag ataatctgga gataccaaat attttagaca atctaggtag
     6421 tggaatttta gaagttaaga atacaactaa aggaacttct tttaaggtca aagtagaatt
     6481 gtcagcaaaa gaagttgatg tattaaaagc tggtggaaaa ttaaactaca ctaaaaatca
     6541 agctaattaa tatgaaaaga gggaatattg aatgtataaa gtgacactta taccaggaga
     6601 tggaatagga ccagaagttg cgaaagctat gaaaaaggta gtagaagcta ctggagtaga
     6661 aatagaatgg gaagaagtta atgcaggaga ggcagttatt gaagagtatg gtactccact
     6721 tcctgaatat attatagata gtataaaaaa gaacaaaata gctataaaag gcccaattac
     6781 tactcctgta ggtaaaggat ttaggagtgt aaatgtaact ttaagacaag cattagattt
     6841 gtatgtcaat ttaagaccta ttaaaagttt taaggggata aaatcaagat atgaagatgt
     6901 tgatttagtg gttgtaagag aaaatacaga agatttatat gctggaatag aacataaaat
     6961 aggtgattat gcagctgaaa gtataaagat aataacaaga agtgcaagtg aaagaatcgt
     7021 tgattttgca tgtaactatg ttaaagataa taagagaaaa aaagttacag ctatacataa
     7081 ggcaaatata atgaaaatgt ctgatggatt gtttttagat gtgtttagag aagttgcatc
     7141 aaaacatggt gtagaatatg atgatttgat tgtagatgca gcagctatga acttagtttt
     7201 aaatccagaa aattatgatg taatggtaat gccaaatctt tatggagata tactatcaga
     7261 tttaggtgct gggcttgttg gaggtttagg tataattcca agtgcaaata ttggtaaaga
     7321 ttgtgcgata tttgaagctg ttcatggaag tgctccacaa atagcaggtc aaaataaggc
     7381 taatccaaca gcacttattc aatcatcagt aatgatgctt agatacttag gtgaatatga
     7441 aaatgcacaa aaaatagaaa cagctttaga aaaagtattt cttgaaggaa gtaaattgac
     7501 tgtagattta ggtggctcag cttctactac agagtttgcg gatgaggtgt gtaagtatat
     7561 tgtatagatt ttatttaggg aaaaagtaaa atatagattg aagaaaagag gctttctcaa
     7621 aatagagata agcctttttt attggaatga taaaatatat ttgattttat acaaaagaga
     7681 gttcctcata aacgaaaaag ttaattttgg gatatccatt ttaattttaa aattaattga
     7741 ttaatatgca aagaaagatt aaataaattt attattttta attgaacttt caaccaaaat
     7801 atggtaaaat ttaaaaaagt tgaactttca actaaaaatg aatatattga ttatgtgaaa
     7861 ttctataaaa cagaaactat ttgactttag ccatagtagt ttcagtggag gtgtgtttga
     7921 ttaagacttt agacagtaat atattaagag aagtaggtac tttatctaga gcagtaaatt
     7981 caataaatga tattaagtac aaggaattaa agttacaaaa aggacaattt acatttttga
     8041 caagaatctg tgaaaatcct ggtataaatc ttgtggaatt atcaaatatg ttaaaggtag
     8101 acaaggctac aacaaccaag gctatacaaa aattaataaa ggcaggatat gtagataaaa
     8161 agcaggatga gtttgataaa agaggatata atttaactcc aactgataaa tctttggaag
     8221 tttatgagtt gataattgaa gaagagaata gaagtataga gatatgtttt gataatttta
     8281 cagatgaaga aaaacaagtg gtaactaagt tattagaaaa aatgagcaaa aacgtagaaa
     8341 atgaatggtt taaagtaaag agatagtaaa taaatacatt aacttaagga gatattttta
     8401 tgattagaaa agcgaatatg aatgatttag aaagcattat gaaaattata aaatctacag
     8461 tggaagaaat gaagacttat aataatacac aatgggatga aaactatcca ttagaaaaag
     8521 actttgtttc agatattaag aaacaagatt tatatattta tgaagtggat ggagaagtag
     8581 cagggtttat atgtcttaat tatgaagagc cagaagaata tttaggctta aattggtcat
     8641 ctaataaaaa agcaatggta atacatagaa tggctgtaaa tccaaacttt agaaagacag
     8701 ggatagcatc taagttagtt gattttgctg aaaaattagc tgtagaaaac aatgtatcat
     8761 acttaaagag cgatacatat tcaataaact caaaaatgaa tttattactt acaaagtgtg
     8821 gttttataaa aatcggagaa atgagttttt taggaaagga gaaatctttc tactgttatg
     8881 ataaaatatt ataaagagca tttacaatac aaggggtaaa taatagattt tcagcattag
     8941 taagggcata atataactta gtatatgcta ttatgaaagg gatgatttat atgattggat
     9001 ttctaaagaa aagaagaagt attagaaaat acaaggatgt tgaagtagaa aaagaaaaat
     9061 tagataaaat attaaaagct gctcttttag ctccatcatc aaaggggctt agaacatggg
     9121 aatttatagt ggtagatgat aaagaaaagc taattaattt atctcaatgt agaactaaag
     9181 gtggtggatt tttcttaaaa aatgcacctt tagctattgt aattattgca gataaagaaa
     9241 aaaatgatgt ttggatagag gatgcaagta tagctgcttc atacatacaa cttcaagctc
     9301 atgaactagg tttaggttct tgttggatac aagtaagaaa tagaatgcat gatgataata
     9361 tagaagctga taagtacatt agagaagaat taaaagtacc tagcaagtac agtgtagaat
     9421 gtataatttc tataggatat cctgatgaag aaaagaaagc atacagtgac tctgatttag
     9481 actataaaaa ggttcatttt aacaactttt agtatataat atgttgtatt aagacattat
     9541 atttttttct attgttaata tatacttaaa attgtaaaaa tatatctgaa ttttactaac
     9601 ttatctaggg gttatgtaca aggattttaa agataaatat agaaattaat gtttataatc
     9661 gatacataac ttgagtaaca aaatttaagg aggttaaagt atgggtatat ttaaaacaaa
     9721 aatggatgag gactggaaag taaactacat taaagagttt aatgaaatga gagattctta
     9781 tgagagtaaa cttcaaaaaa aacagtttga ggtagatagt ttgaaatcag aattggatag
     9841 actaagaagc tataaaaata gccttaagcc aaaagaaaaa caaattactg atgatgatat
     9901 aaataatata aagagtttaa gaagagatgg cttaagctat aaagaaattt caaatcaaac
     9961 aagttggagc aaagcaactg taagtagggt attaaatgga ctttacgatt agcatataca
    10021 tggggtgatt taaattggga tgaagtatag ttacaaaaaa tgcataatag attctatttt
    10081 attgatgttt atagttcaaa tattaaggat gattttgaat tatgtacttc taagtcaatt
    10141 tgaatttact cttgaaaatt ttaatattat aaacttaatt tcttttacat tagttggttt
    10201 atcgttaatt ttatttttaa aagataattc tttgtataac aaggttagaa atagaaaaat
    10261 cacagaagct tttgaagaaa ataaaaacaa tatacttatt gaaaagtgta agctgatact
    10321 ttttgttgtg gttttatctt tagcaataat tgtaacttat tgtactaagg gatatgtact
    10381 ttttaatgta actatgatga ctttgtctgt attgatagtt ccaatatttg aagagttatt
    10441 ttttagagaa tatatatgga actatctaag taattttata aagtcaaaga gtaaaattat
    10501 ctgtataaca tctatactat ctggaattta taatatagga tacatagatg ttataagaaa
    10561 ttatgtaata ttgtataaca attcgagcta cacctttgaa gttattattt caaaaattat
    10621 gataggtact gtttttggta ttgtacttgg attagttaag tataggttta gagatgtagg
    10681 gttttgtata ttattgagga gcttatttgc tatatttatt aggtagatag tataatcggt
    10741 ttataaggtt gttttaaatt t
//
LOCUS       DAEOSP010000133        10664 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.135, whole genome shotgun sequence.
ACCESSION   DAEOSP010000133 DAEOSP010000000
VERSION     DAEOSP010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.135"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            72..419
                     /locus_tag="KRH54_002596"
     CDS             72..419
                     /locus_tag="KRH54_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBG8580241.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(569..1069)
                     /locus_tag="KRH54_002597"
                     /pseudo
     CDS             complement(569..1069)
                     /locus_tag="KRH54_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(1096..2601)
                     /locus_tag="KRH54_002598"
     CDS             complement(1096..2601)
                     /locus_tag="KRH54_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG8580242.1"
                     /translation="MNVAIYLRKSRADEEAEHRGEFETLSRHKTTLLKLAREQNLNVI
                     EIKEELVSGESIEYRPKMLELLGEIKNNNYDAVLVMDIDRLGRGNMQDQGLILDTFKK
                     SKTKIITPRKTYDLNDEFDEEYSEFEAFMARKELKLISRRMQRGKIKSIEEGKFIAQA
                     PPYGYDIKYLNNKERVLVINEDKATVVKIIFDMYLKGDGACKISNYLNELGYKTTTGR
                     TWYEKGIRDIIKNKVYCGYIVWNKVERKRNSSRTRGIEEQIEVKGKHEPIISEDIWNK
                     AQNIRKKRINSPTPNNKITNPLAGIVKCGYCNHTLIAKSNTTSNGYEKYMVCTNCKNK
                     ATKLYILENKILEVLNKWLVNYRLSLKDEKDNSKPENTLEHTLTNLNKELDTLEKQKE
                     RLHDFLEQGIYDNNTFGERLKILLSRIENVREAINKTNIDIEMQKERNTVKTQIVPQL
                     EKILSEYTEEKEPYLKNILLKSVINYAVYKKEPNQKSDDFSLILYPNIPRD"
     gene            complement(2800..3654)
                     /locus_tag="KRH54_002599"
     CDS             complement(2800..3654)
                     /locus_tag="KRH54_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG8580243.1"
                     /translation="MEWIERLNKAINYIEENITKEIEYEQVAKIACCSTYHFQRMFAY
                     MADVPLSEYIRRRRMSLAAVELQNDDKKIIDVALKYGYSSPTAFNRAFKSIHGVAPSV
                     IKKREITTLKAFPPISFKISIKGAEEMNYRIEKKEAFRIVGVSQPLHNELEKNFEIVP
                     QMWQKVALDGTLQKLIPMMDSQLQGVLGISICNNSEEWKYFISVSSTKSIDNTLEEYT
                     VPAFTWAIFSGEGQCPQAIQELEKRIVTEWLPTSGYEYDNGPDIELYLNPDPQNAKFE
                     VWIPVVKK"
     gene            4039..4320
                     /locus_tag="KRH54_002600"
     CDS             4039..4320
                     /locus_tag="KRH54_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580244.1"
                     /translation="MPNIEKDLDGTTTITYLNDDIGVPTDENGYKISMTILHGKTICH
                     VVSPETILGRKFTLEEKQEKDTIIYGAIEKIMVDRQLQIKNSNKEVASH"
     gene            4470..5318
                     /locus_tag="KRH54_002601"
     CDS             4470..5318
                     /locus_tag="KRH54_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBG8580245.1"
                     /translation="MIKSFLMLGQSNMAGRGFISEVTPIYNERIQMLRNGRWQMMTEP
                     INYDRPVSGVSLAASFADAWCCENQEDRIGLIPCAEGGSSLDEWNIDGILFKHAISEA
                     KFAIQSSELTGILWHQGENDSNNGNYKFYYKKLLSIIETLRKELNIPDIPIIIGGLGD
                     FLGKVGFGKSCTEYVFINQELQKFAFEQDNCYFVTATGLTSNPDGIHIDAISQRKFGL
                     RYFEAFHKKKHIMEALANESELIIPSNSKTYTKTEKIYINSMDLALGKISYDEFESKL
                     IKLNDN"
     gene            5370..5891
                     /locus_tag="KRH54_002602"
     CDS             5370..5891
                     /locus_tag="KRH54_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8580246.1"
                     /translation="MIPLLILGLLKQNPNSYGYELLALMEKKHYKYIVNFTKGSFYYN
                     LQQLEEKKFIEKVENKQSNKNRETHNYIITELGCQEFEKLMYKYGSKTEYINLSFYAT
                     LLFANDYKNEKLIELLETQIKQTQVRISLLNESIENDKTNSHYFNRMLENSRSHHLVN
                     IEWFEKLLNDIRD"
     gene            6242..6775
                     /locus_tag="KRH54_002603"
     CDS             6242..6775
                     /locus_tag="KRH54_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBG8580247.1"
                     /translation="MTEVIIVNDARIISQIKAGKKELFDTLIKKYYDSIYCYCYRHVD
                     NKMTAQDLTQDVFVTVINNIENYKHYGKFENYLYVIAKNKCKDFWKKKQPIYLESLDK
                     EISNQELSDIENKMFIKEALTKLPELEFEVIILRYYHDLKFKDIAKIMESSVSVTKYR
                     MSRALKKLELYLEGGKL"
     gene            6772..7533
                     /locus_tag="KRH54_002604"
     CDS             6772..7533
                     /locus_tag="KRH54_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580248.1"
                     /translation="MNNKEIKRSLQKYKVPKYDSSALEKTIKTIHELTLSNTYKRMNN
                     KDFFISQIGFIRKRTWIMQFIILCTICIFLFSTSADSNSFYNTLSLISIASPLFVLTN
                     IEELTKVYNSSILEIEFSTKNTLHKIIATRMLIFGISDLLFMIIILVSAKQIIDVNIL
                     YIIIYILVPFNLMCIGCMEIINRNKGKHTNYYCMIYGISLVLLIVTLSNFRDNLYQFE
                     AINQWLIVFLVTAVTLIYEIRKTWRHLCSFENIII"
     gene            7571..8437
                     /locus_tag="KRH54_002605"
     CDS             7571..8437
                     /locus_tag="KRH54_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580249.1"
                     /translation="MELSVERLTKQYNNKIIVDKIDFKLNEGIYGFLGENGAGKTTLM
                     RMICGILSPTSGEVKVDGKNNLDMGEDFRDMIGYLPQNFGYYPDFTAKEFMLYIASLK
                     GLMPSYAKRKTQELLEMVGLSEVINKKIRTFSGGMKQRLGFAQTLLNDPKILILDEPT
                     AGLDPKERVKFRNLISEFSKEKIVILSTHIVSDVEYIADKILIMKKGRFVLTGSVEEV
                     TSKIKDKVWKCVVNENEIDYMSDRYCLANIHHSGGVANLRIVSDIKPHDNACNLKPTL
                     EDLYLYCFNEVL"
     gene            8458..9699
                     /locus_tag="KRH54_002606"
     CDS             8458..9699
                     /locus_tag="KRH54_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG8580250.1"
                     /translation="MSTLIKFELKKIIKRKSTIAVILGSLFLSIILFALMPIQEKAND
                     ESGKEYRGLQAIELKKQQSKLNEGYITDKKVKNSIRDYQKMFNNPNNIVTNESGFSDF
                     KPDIYWKFFYPNKTFYNFIIESYSKPNDFNPEVIKSMPLENEINFYKFRNEKVNTILN
                     MEYPEGNYTQKEKDFWLSKNKDIKEPYLNGYYQGWEYFLLARDLLIFTIMAVCISIAS
                     VFSGEYQSGADSVVLTSKYGKTKVITAKIISSFLFGTIIFTINTIVSIGILLLSFGTD
                     GWNLPLQLTTSSIPYPYTFLQAILLSVLTIYMVLIVLISFTLLLSSYMKSPFPVLITN
                     IILLFVPIFFKYSETNKLYNQILNVLPSNAISFSFTNYVDYKFGNLIIALPTMIIFTC
                     LVLTVVFLPFIKNNFKKHQVN"
     gene            complement(9923..10495)
                     /locus_tag="KRH54_002607"
     CDS             complement(9923..10495)
                     /locus_tag="KRH54_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG8580251.1"
                     /translation="MFKKIVAMASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQKSV"
BASE COUNT         3933 a         1372 c         1474 g         3885 t
ORIGIN      
        1 aagtgagaat actattttct tatattggta taataatagt aaatctaata tagaaaagag
       61 gtatttctag tatgcatgaa aataaaaaga atctattaga cggaagctta gctcaatata
      121 atgatacagc agttccaaaa ataccagtat ttgctggtaa cgatataact tcagaagttt
      181 attattttaa acctaatcaa gttcttaatg ctcatagaca ccctaatggt gaacaaatat
      241 ttgtgttcct aaaaggagaa ggtaaaatga aacttggtga gcatgaatgt gatgtaaaaa
      301 acggcgatac tgtatttgta ccaacaggag agtggcatga aattactaat ggaagcaatg
      361 aagaaatggt agccgttcaa attacaaaaa ttaatgctgg agcagaatac agaggataga
      421 tatataagta cagttagtac tttaatatct attttattat ataaacttta atctcaaata
      481 taaaaagagt cttttaaaag gctcttttta tatttttgtt aattttcatt ataaaaacaa
      541 gaaaatatct agctaaaact aatatatttt atttaatatt ttttatagca ttgtttgcaa
      601 actcagtatt tacgatatct ttataaggtg cttccttatc tagttcttta gcttctttca
      661 taacagtcat caatttcttc aaaccttcct cttttaatac aggattttga gaccatgcat
      721 ctattgattt atatctatct acaactgttg ttaaatcttc taaagatata tcttcaaaga
      781 aaggttgcat agccttagct acttctttgc tagatgcttg ttgaatccat aattgacctt
      841 tataaacagc atttgtaaat ctttgaatta aatctttatt gtttttcatg tatgatctcg
      901 tagtagagta tgctgtaaaa ggtatttctc cagaatcttt tcctatagat gtgactatat
      961 atccactacc ttctttttcc attgctgttg ctgtaggttc aaatgctgtt gtaaagtcac
     1021 cctctccacc tgcaaaagct ccagccatta caccaaattg gtaatcagtc tatttataac
     1081 atttaaaaca tataattaat ctcttgggat attagggtaa agtattaaag aaaaatcatc
     1141 agatttttga ttaggttctt ttttatagac tgcataattt attacacttt taagcaatat
     1201 attttttaga taaggttctt tttcctctgt atattcactt aaaatttttt caagttgagg
     1261 aacaatttgt gttttaactg tatttctttc cttttgcatt tcaatatcta tatttgtttt
     1321 attaatagct tctcttacat tttctatacg agataagagt atttttaaac gttccccaaa
     1381 tgtattattg tcataaattc cttgttctaa aaaatcatgt agtcgttctt tttgtttttc
     1441 taaagtatcc aattctttat ttaaatttgt caaggtatgt tctaaagtat tttcaggttt
     1501 agagttatct ttttcatctt ttaaagatag tctataattt actaaccatt tatttaagac
     1561 ctctaatatt ttattttcta atatgtatag tttagtagct ttatttttac aattagtaca
     1621 aaccatatat ttctcatatc catttgatgt agtattagat ttggctatta aagtatgatt
     1681 acagtatcca cattttacaa ttccagccaa tggatttgtt attttattgt ttggagtagg
     1741 ggaattaata cgtttttttc ttatattttg tgccttattc catatgtctt cacttattat
     1801 tggttcatgc tttcctttta cctcaatttg ttcctcaatt cctcttgttc tactagaatt
     1861 tcttttcctc tcgactttat tccatactat atatccacaa tacactttgt tttttataat
     1921 atctcttata cccttttcat accaagttct acctgttgtg gtcttataac caagttcgtt
     1981 taaataattg gatattttac atgcaccatc tccttttaaa tacatatcaa agattatttt
     2041 aacaacagtt gctttatctt catttattac aagtactctt tctttattat ttaaatattt
     2101 aatatcatac ccataaggag gagcttgagc tataaattta ccctcttcaa tacttttgat
     2161 ttttcctctt tgcatacgtc tagatattaa ttttaattcc ttacgagcca taaaagcttc
     2221 aaattcagaa tactcctcat caaactcatc atttaaatca tacgtttttc taggagtaat
     2281 aattttcgtt ttagattttt taaatgtatc tagtattaac ccttggtctt gcatattacc
     2341 acgacccaaa cggtcaatat ccatcactaa aacagcatca tagttattat ttttaatttc
     2401 acctagtaat tctagcatct taggtctata ttctatactt tccccagaaa caagttcttc
     2461 tttaatttca atgacattta aattttgttc tcttgctagt tttaaaagag ttgtcttatg
     2521 tctacttaga gtttcaaatt cacctctatg ttctgcttct tcatcagctc tgcttttgcg
     2581 taaataaatt gccacattca ttaaatcaca ccccttgaat atattatatt cagtaacaga
     2641 ttgattatat ttaaaacaca aaaataaacc ttcaaaatac aagatatatt ttttaggttt
     2701 atttttatac agtaaatttt atagtatatt taaaagcttg taatctaagt aaatatttat
     2761 actattaatt ttatgctttc ttcatttttt aattttaaac tattttttta ctacaggtat
     2821 ccatacttca aatttagcat tttgagggtc tggatttaag tacagttcaa tatcaggacc
     2881 attatcatat tcataaccag aagtaggtaa ccattctgtt acaatacgct tttctagttc
     2941 ttgtattgct tgtggacatt gtccttctcc agaaaatatt gcccaagtaa aagctggaac
     3001 agtgtattct tctaatgtat tatctataga ttttgtactt gaaacagata taaaatactt
     3061 ccattcttct gaattattac aaatacttat acctaataca ccttgaagtt gactatccat
     3121 catagggatt aatttttgaa gcgttccatc taaagcaacc ttttgccaca tttggggaac
     3181 aatttcaaaa ttcttttcta gttcattatg aagtggctga gatacaccaa ctatacgaaa
     3241 tgcttctttc ttttcaattc tataattcat ttcttcggct cctttaatac taattttaaa
     3301 actgattgga ggaaaggctt ttaaagttgt tatttctctt ttttttataa ctgatggggc
     3361 aacaccatga atacttttaa atgcacggtt gaatgctgtt ggagaagagt atccatattt
     3421 taaagccaca tcaattatct ttttatcatc attttgtaat tctacagctg ctaaagacat
     3481 ccgtctacga cgaatatatt cagaaagtgg tacatctgcc atataagcaa acattctttg
     3541 aaaatgatat gtagaacaac aagctatttt tgccacttgt tcatactcaa tttcttttgt
     3601 tatgttttct tcaatatagt taattgcttt gtttaatctt tcaatccatt ccataaaatc
     3661 agctccttgt aatttatata atatctattt taaaatagtt aatcctctct ttctatgcac
     3721 taattaacta gcttaataaa gaatgaacat gagaaagtct gtaattaact acaaaaatga
     3781 acaaatagta ttgtttttca cattaaagta tatcacagat tagtatctaa tttttatata
     3841 agataacagt tttaatttat tatgaaattt ttataatatt ttagcttaga catttaatta
     3901 tatataacat tttattcata gttaactatt tttgaagttt acccttaatc aagcaacact
     3961 tagttactca attataaata atgattgaga ataggacaat ttttaagata cataaaatat
     4021 aaagaggagg ggatttaaat gccaaatata gaaaaagact tagatggtac aactactata
     4081 acatacctca atgacgatat aggtgttcca acagacgaga atggatataa aatatctatg
     4141 acaatactac atggaaagac aatctgccat gtagtatctc ctgaaacaat cttaggaaga
     4201 aagttcacat tagaagaaaa acaagaaaaa gatacaataa tatatggagc aatagaaaaa
     4261 ataatggtag acagacaatt acaaataaaa aattcaaaca aagaagttgc tagtcattag
     4321 cacttcttta ataagtaaag taggacaagt attttttata atattattga cttatagtct
     4381 aattagacta taatataaac atagatagtc taattagact actattaaaa ataaatacaa
     4441 taagaattta agaaaggata ataataataa tgattaaatc atttttaatg ttaggacagt
     4501 ccaatatggc tggaagagga ttcataagtg aagtgactcc aatttataat gaaagaatac
     4561 agatgttacg taatggaaga tggcaaatga tgacagaacc tattaattat gacagaccag
     4621 tttctggggt aagtcttgct gcatcatttg cagatgcatg gtgctgtgaa aatcaagagg
     4681 atagaattgg gctaattcct tgtgctgagg gaggcagttc cttagatgaa tggaatatag
     4741 atggtatact ttttaaacat gctataagtg aagctaaatt tgccatacaa agcagtgaat
     4801 tgacaggaat tttatggcat cagggagaaa atgacagtaa taatggcaac tataaatttt
     4861 attataaaaa attactttct attattgaaa ctcttagaaa agaattaaat attccagata
     4921 taccaattat tattggtgga ttgggagact tcttaggaaa agtaggtttt ggaaaaagtt
     4981 gtactgagta tgtatttatc aatcaagagt tacagaaatt tgcttttgaa caagataatt
     5041 gttactttgt tacagcaaca ggtcttacat ctaatcctga tggtattcat attgatgcaa
     5101 tatctcaaag aaaatttgga ttacgttatt ttgaagcttt tcataagaaa aagcatatta
     5161 tggaagcatt agctaatgaa agtgagttga taataccgag taattccaag acatatacaa
     5221 aaacagaaaa aatatatata aatagtatgg acttggcact aggaaagata tcatatgatg
     5281 agtttgaatc taaacttatt aaacttaatg ataattagat aaaatattca ttaatatact
     5341 aataaatata gattgaaaga ggtgattttt tgataccatt attaatttta ggattattaa
     5401 aacaaaatcc aaattcttat ggatatgagt tattagcatt aatggaaaag aagcattaca
     5461 agtacatagt aaatttcact aaaggttctt tttattataa tttacagcaa cttgaagaaa
     5521 aaaagtttat tgaaaaagtt gaaaataaac aatctaataa aaacagagaa actcataact
     5581 acataattac tgaacttgga tgccaggaat ttgaaaaatt aatgtataag tatggttcta
     5641 aaacagagta tataaatcta tcattttacg caacactatt atttgcaaat gattataaaa
     5701 atgaaaagtt aatagaatta ttggaaacgc aaattaagca aactcaagta agaatttctc
     5761 tattaaatga atccatagaa aatgacaaaa caaattcaca ttattttaat agaatgctag
     5821 aaaattctcg ttctcaccat ttagttaata ttgaatggtt tgagaagttg ctaaatgata
     5881 ttagagattg attttaataa taaaaaaaca tacatatata ctttatataa atgtatgata
     5941 tatagattaa aaataaggca gataattttc aattgaatat ctgtcttttt gttatataag
     6001 atgttaaaag acacttatac tatgtaagta aaatttataa atataaagtt gttcattgtt
     6061 gaattacaga cacttaagat gaaaagtatg acatttgata taataaaatt gtttaagtat
     6121 taattatgag tattaattaa tggtatagat aattttaata taactgtatt tataaataat
     6181 tataaaataa taaattaatt tttttatact gactttttta tagtataaat cgttatatat
     6241 aatgacggag gtgataatag tgaatgatgc tcggattata tcacaaataa aagcaggaaa
     6301 aaaagaattg tttgatactc taataaaaaa atactatgat agcatctatt gttattgtta
     6361 tcgccatgta gacaataaaa tgactgcaca agatttaact caagatgtat ttgtaacagt
     6421 tataaataat atagaaaatt ataaacatta tggtaagttt gaaaactatt tatatgttat
     6481 tgcaaaaaac aagtgtaagg atttttggaa gaaaaaacaa ccgatatatt tagagtcatt
     6541 agataaggag atatcaaatc aagaattgtc tgatatagaa aataaaatgt ttataaaaga
     6601 agctttgact aaattaccag aattagaatt tgaagtgata attttacggt attaccatga
     6661 tttaaaattt aaggatattg ctaaaattat ggagtcaagc gtctctgtca caaagtacag
     6721 gatgagtagg gcattaaaaa aactagaatt atatctggaa ggaggtaaat tatgaataat
     6781 aaagaaataa aaagaagtct acagaagtat aaggttccca aatatgattc ttcagctctt
     6841 gaaaagacta ttaaaactat acacgagtta acgcttagta atacttataa aagaatgaat
     6901 aataaagact tttttatatc tcaaattggt tttataagaa aaagaacttg gattatgcaa
     6961 tttataatac tatgtacaat atgtatattt ttatttagta catcagcaga tagtaattca
     7021 ttttataata ctttatcctt gatttctata gcatcaccac tttttgtgct gacaaatata
     7081 gaagaattaa caaaagtata taatagtagt attttagaga tagaattttc aacaaaaaat
     7141 acattacata aaattattgc tactagaatg cttatatttg gaataagtga tttattgttt
     7201 atgattatta ttttggtatc tgcaaaacaa atcattgatg taaacattct gtatataata
     7261 atttatatac tagttccttt taacctaatg tgtataggat gtatggaaat tataaataga
     7321 aataaaggta agcatacaaa ttattattgt atgatatatg gtatatctct tgtattatta
     7381 atagttacac tgagtaactt tagagataat ctatatcaat ttgaagcaat aaatcaatgg
     7441 ctaattgttt ttctagtcac agcagtaaca ttaatatatg aaataagaaa aacgtggaga
     7501 catctttgtt catttgaaaa tattattatc taagtaaaaa ataagaatac acagtatcag
     7561 gaggaatagt atggagttaa gtgtagaacg tttaacaaaa cagtataaca ataaaattat
     7621 agtagataaa attgacttta aattaaatga gggtatatat ggatttttag gagaaaatgg
     7681 agcgggaaaa acaactctta tgagaatgat ttgcggaata ttaagtccta catctggaga
     7741 agtaaaggtg gatggtaaaa ataatttgga tatgggagaa gattttagag atatgattgg
     7801 ataccttcct caaaactttg gatactaccc agattttact gccaaagaat ttatgctata
     7861 tatagcatca ttaaaaggat taatgcctag ctatgcaaag agaaaaacac aagaattatt
     7921 agaaatggta ggattatcag aggtaattaa taaaaaaata agaacatttt ctggaggtat
     7981 gaagcaaagg ctaggatttg ctcaaacact acttaatgac ccaaaaattt taatattaga
     8041 tgagccaaca gcagggctag accctaaaga acgagttaaa tttcgcaacc taatttcaga
     8101 attttctaag gaaaagattg ttatattgtc tactcatatt gtgtctgatg tagagtatat
     8161 tgcagataaa attttaataa tgaaaaaagg aagatttgtt ttaactggct cagtagaaga
     8221 agtaacttca aagataaaag ataaggtatg gaaatgtgtg gtcaatgaga atgaaataga
     8281 ttatatgtct gatagatatt gtttagcaaa tatccatcat tctggtggag ttgcaaatct
     8341 tagaattgta agtgatataa agccacatga caatgcatgc aatttaaaac caacattaga
     8401 agatttatat ttatattgtt tcaacgaagt actataaata tacataagga gataggtatg
     8461 tctacattaa ttaaatttga gttaaaaaaa ataataaaaa gaaagtctac aatagcagtt
     8521 attttaggaa gtttattctt aagcatcatt ctttttgcat taatgccaat tcaggaaaaa
     8581 gctaatgatg aaagtggaaa agagtatagg ggtttacagg ctattgagct aaaaaagcaa
     8641 caatctaaat taaatgaggg atacataaca gataaaaaag taaaaaactc aatcagagat
     8701 taccaaaaaa tgtttaataa tccaaataac attgtaacaa atgagtctgg attctcagat
     8761 tttaagccag atatttattg gaaattcttc tatccaaata aaacttttta taattttata
     8821 atagagtcat atagtaaacc aaatgatttt aacccagaag taattaaatc aatgccatta
     8881 gaaaatgaaa ttaattttta taaatttaga aatgaaaaag taaatacaat attaaatatg
     8941 gaatatccag agggaaatta cacacagaaa gaaaaggact tttggttatc taaaaataag
     9001 gatataaagg agccttattt aaatggatat tatcaagggt gggaatattt tctattggca
     9061 agagatttac tgatttttac tataatggct gtctgtataa gtatagcttc tgtattttca
     9121 ggagaatacc aatctggtgc tgattcagta gtattgactt ctaaatatgg aaaaacaaaa
     9181 gttataacag ctaaaataat aagttccttc ttatttggta cgattatttt tacaataaat
     9241 acaattgtat ctattggcat acttctttta tcttttggaa ctgatggctg gaatttacca
     9301 ctacaactta caactagttc aattccatac ccatatacat ttttacaggc aatattacta
     9361 agtgtgttaa ctatctatat ggtattaata gtattaatta gttttacact tttattatcg
     9421 tcttatatga aatctccttt tccagtttta attacaaata taatattgtt atttgtacca
     9481 atatttttca aatacagtga aacaaataag ttgtataatc aaattctcaa tgttttacca
     9541 tcaaatgcta tttcattttc atttactaat tatgtagatt ataaatttgg taatttaata
     9601 atagctttac ctacaatgat tatttttaca tgcttagttc ttacagttgt ttttttacct
     9661 tttattaaaa ataattttaa aaaacatcaa gttaactaaa atatatatta taaaaagaag
     9721 gtgtcttaaa atgaactttt tagcttatta ggcactcttc tttgtattaa tttaaatata
     9781 ttttagtatt agattaaata tgttttcatc attttaacaa tgaaaaagag ccttatctct
     9841 attttgagat agctcttata aagttaaata ttaaattgat aactataaaa tatgtagttt
     9901 gaatttataa tttgtgttat aatcatactg atttttgtac atcagttctt acattaacat
     9961 tgcctgcttt tagattttct ccaatagtaa gacctttttc ttttagaaca tattcaaaag
    10021 tcattagtgg aactccacct tttctaccac ctaatatttc ggttcctttt aaatcattgt
    10081 aagagaaatt aggcattttt ttcctagcaa ctagaaaact accatctctt tttgtcaatt
    10141 gagcaaagtt cactgcataa tcgtttttgt tttgattata tacatatata gatgcttcag
    10201 gacccattaa acctacgttt gcttcacctg aaatcaaagc tgccatagtt ttatctgcac
    10261 cttgggtatt tataaggtca atctttatac cttcatcttc aaagaatcct ttcgtaatag
    10321 cagcatactg tggagcataa aaaacagagt gagtaacttc tgctatagtt actttatcca
    10381 gatttttaga cgaagaagat tcatcttttt tgtcattaga gttaaaatca catccagtta
    10441 gacatattgt acttacaatg atagagcttg ccattgcgac tattttttta aacataataa
    10501 atacctccca taagacatac gaatagtata tagtattaaa aatttattgt agttgttaca
    10561 aaaatgtatc tatgcttata atatttctac aaaaacttaa taataatttt cagttttatg
    10621 tttagttttt ttagtaatgt gtaatattgg ataaattata ttga
//
LOCUS       DAEOSP010000134        10646 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.136, whole genome shotgun sequence.
ACCESSION   DAEOSP010000134 DAEOSP010000000
VERSION     DAEOSP010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.136"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            102..1262
                     /locus_tag="KRH54_002608"
     CDS             102..1262
                     /locus_tag="KRH54_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBG8580252.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1604..1765
                     /locus_tag="KRH54_002609"
     CDS             1604..1765
                     /locus_tag="KRH54_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580253.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            1958..2587
                     /locus_tag="KRH54_002610"
     CDS             1958..2587
                     /locus_tag="KRH54_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBG8580254.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2826..4085
                     /locus_tag="KRH54_002611"
     CDS             2826..4085
                     /locus_tag="KRH54_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBG8580255.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4344..5339
                     /locus_tag="KRH54_002612"
     CDS             4344..5339
                     /locus_tag="KRH54_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBG8580256.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5585..6652
                     /locus_tag="KRH54_002613"
     CDS             5585..6652
                     /locus_tag="KRH54_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBG8580257.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFVEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7186..9096
                     /locus_tag="KRH54_002614"
     CDS             7186..9096
                     /locus_tag="KRH54_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBG8580258.1"
                     /translation="MNSNTFERLQLNEVKELIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMLF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFVSVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9159..9782)
                     /gene="ytaF"
                     /locus_tag="KRH54_002615"
     CDS             complement(9159..9782)
                     /gene="ytaF"
                     /locus_tag="KRH54_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBG8580259.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            9970..10614
                     /locus_tag="KRH54_002616"
     CDS             9970..10614
                     /locus_tag="KRH54_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8580260.1"
                     /translation="MEQNNKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
BASE COUNT         4359 a         1053 c         1749 g         3485 t
ORIGIN      
        1 atagataaaa gtatttattt taattgtcta ttatctgaaa aagtctttac ttagaaatca
       61 taaaaatcaa attaattagg aaaagaaagg gtgtgtataa tgtgattgag actggatttg
      121 tatattgtag tttcttggtt gtattcgtag gtgtaatttt ttttatggtt gataaattga
      181 aatggaaggt atttgatgtt attaatccaa tgcttttaat atacttaggt tcagctatac
      241 ttggatcatt tggtttattt gcacagaatg aagatgttgg aacgtatcaa aacataataa
      301 cagtgaactt ccttccatta atgttagtgt ttttgttaat tcaatgtgat ttaagaaaaa
      361 tattaaagat ggggcctaaa atgttattat catttttctg tgctacattt acatttatgc
      421 ttggatttgt tatagcattc ataattttta aaaattcaat gggtgcagat tcatggaaaa
      481 ttcttggggc agcatctggt tcatggatag gtggctcatc aaatatgatt gcagcagcta
      541 atgctctagg agttgcagaa gaaggactta gttatgcaat acttatgggt tctataggat
      601 atacagtatg gatgtctgtt tgtctaatgt ctgtgaaatt tggacctaaa tttaacaaat
      661 ggacaaagtg cgatacaagt tttatagatg aggttacatc aaaacttgaa aaagaaaata
      721 aagataatac ggcacctacg tttgtagaac ttatgatttt actttctgtt gggtttggag
      781 ttgcgtcttt tgcacaaatt gtatccaaga tacttccaac ttcaggtata cttaatgaaa
      841 ctatgtgggt agtacttgtt gcatctacag tatcaatatt attgggtatg acaaaaatat
      901 caaagcttaa agggagtaca ctggtaggaa atgtgtttat gtatttactc cttgcagcta
      961 caggtagtaa agcaaatttc tttggattgt cagaagcacc tgtttacata atgtttggat
     1021 tggtagtagt ccttgtacat gctatttcat ttatagggct tgctaaacta tttaaacttg
     1081 atttatttac ttgtgaagta ggaagtatag ctaatatagg aggagtaact acagctccaa
     1141 tgattgctgg ggttcacaat cctgtattaa taccagtagg tgtacttatg gggcttctag
     1201 gaaatattat agggacttat tgtgctctta tggtaactca aatattacat atgttagctt
     1261 aataaaaata tggtataaaa ttaataattt aaataagaaa agtcatttca atttaagcag
     1321 aagcttttac tgaaatggct tttttgtttg acaagataat ctatttcaag aagatgtttt
     1381 gacatattta gtgtataata ttgtaaaatt tactaaaaac gagatatatt taatttttat
     1441 attgaatcga acgtctgtat gatgtataat aaatatacag aacgaatgtt tgtagaacgg
     1501 gtgttttttg aatttatttt aataatttat gaatatatat cagaatgcat aaatatattt
     1561 aaatataaag attaaaaaga atttaaagga gtgttgataa cttatgataa ttccatcaag
     1621 aagaatacaa gatataacat taacaaattt aaaaaatggt gaagtaactt taattgaatt
     1681 ggatgaaata tacaagaaga tgggattttt atttgttgtt agtgaaggca aattgaaaaa
     1741 gataaaaaag gagaatcatc attagaaatg aatgaaataa taaatattat aatagtttat
     1801 aaaacctaac ttatgtagaa tataaatact taagttaggt ttttttgtga taaagtttat
     1861 aagtactcaa ttttataaga taaataatgg tcttaagtga aaagtaaata agtttaaatt
     1921 ttagtaagta ttatattgca taagaggtga tattattatg aaaatagtaa cctacaatat
     1981 tcataaaggt atggattcaa ataatagatt aactctaact aaaatggggt tatatttaaa
     2041 acaattggat tgtgatgtaa tttgtttgca agaagtatta tatcctcaat ttttagcttt
     2101 aaaagctgtt ttaaatatgg atggtgtctt tgcgacaaat gtaaaaaaag taaatatgat
     2161 atatggtatt tgtacattta caacatctaa aatgttaaac agtaatcatt ttttcttaac
     2221 tagcaaaaaa gaacaacggg gagctctttg tataactata gatgcatatg gaagaattat
     2281 aaatgtaata aatactcact tgggacttga taggcaggag agagcaaaac aattagacga
     2341 aataatagac tataggaaca gattagtggg gatagttatt ttatgtggtg attttaatga
     2401 gaaaaatgtc tttttaagta tgtttaatga tatggctatt tcactaaaca agtcttactt
     2461 accaacattt gaaaaatcaa actctagaat agattacatt tttgttaata aaaatactga
     2521 actaaaagga tacacagtag aaaaaattta cctgtcagac cattatcctg taataggtta
     2581 catttaaatt tcaatataaa tgaaattagt tgttataaaa taaacaattt tttgttaaga
     2641 aatagtaaaa gaaatataat aaaaaatatc aaaaataaaa ctaattataa gacataaatt
     2701 tacaataaag ttacatttga ttaaattcct tgtttaacaa aagctattca aatataatct
     2761 aaggtagttt tgaaatggga actctattat gcaaatttaa gctagtgtta aaatgaggtg
     2821 aagttatggg cagaagaaca acaagagttt caagattagg taaagaaaaa aaacgaagtg
     2881 ttttaattgt attggcagct ttagttctaa taatgggtgg atttacttat ttttttaaca
     2941 gtaaatttct gtacaatggg aaaatcgcta aaaatgtata tatagaagga gtaaatgtat
     3001 ctgatatgac taaagcagag gcattaaaag ctataacaga taagtatact ccagaagatt
     3061 taaatcttgc ttatgatggg aaaaaatata caatatcacc aaaagatatt gatttaaaat
     3121 atgatacaga agatgttgta aaagatgctt atgaaagtac aaaaaaaggg tcctattttc
     3181 aaaacttaaa aaaatatatt gatataagag tgaataaggc aaatatgaag ataaaagctg
     3241 aatatgatga ggcaaaactt agttccaaag tgtcttctat agcagatagt ataaatgtta
     3301 agatgaaaaa tgcttctatt agtgttggaa gtggtggact aagttataca gactctgttg
     3361 taggtagaga atttgatttg gcagcaaata aagagtctat atataatatg ataaaaaata
     3421 aagagcataa aactttagaa cttaaagtaa atcttcaaaa accagatata acaactgaac
     3481 aagtaaaaac tgtaaattct gttataggtc aatattcaac tacttattca caagctgtag
     3541 agggtaggtc atataatgta ggattatcag caagaaaaac tagtgatgta ttattaatgc
     3601 caggtgaaga attttcatac aataaactta caggacctag taataaagca aatggttaca
     3661 aagatgcacc tgttatagtt tatggtaagc tagaacaatc agcaggagga ggtgtttgtc
     3721 aaacatcttc aacagtttat aatgcggctc ttctttcggg tatggagata actcaagtta
     3781 caaatcattc atctgcatct acttatgtgc ctaaagggag agatgctact gtgagcgatg
     3841 gaggattaaa cttaaagttt aaaaatccat ataagcatcc tgtatatatt aaaaattatg
     3901 caggtggagg aagtgtatca tcagtaatct atggtaattc tggtgacaaa ccaaatatat
     3961 ctatagaggt aaaacaaact ggtcagaata aatattctac atatcgaata tttaaagatt
     4021 caagtggaaa ggtcataaaa aaagaacata tatcaaatag ttcttataaa gaattaaaaa
     4081 aatagataat tttttagaga aatatttgta atataaaagg gagtgtctca aaatgaactt
     4141 tttagtttat gaggcactct ttttctgtga aatcaaatat attttcatca tttcaacaat
     4201 acaaaagaag cgtatctcta ttttgagaca gccttttttg taaaaattaa cactattagt
     4261 tcttgtttta atattacaat cattaatata tagaaaatat tcctatatat tggtataatt
     4321 gtaaatagat ttagttatat aaaatgtatg aggaggtgac atggttgaat tttataggca
     4381 atagatatga ggtagtaaat tctgacgacg ttttagaaat taacaaaatc tataaagcta
     4441 gagatgtttt ttacaggaaa aatgtgttaa taaaagttat aaagcacaat aattatatct
     4501 gtgaagattt tgtttcaaat ttaattgatg aaagtactgc tttagatgag ataaactctc
     4561 cttatatact aaaaataata gatgttggga ttcactgtac agaagaaaca acattatatt
     4621 atgttgtaag tgaagacttt aatggtatcg gactagatga attaattcta ggtaattatc
     4681 ttcatttaga agcaattgta aacataatga tacaagtttt aaaagcttta gaaatgatac
     4741 ataggaatca ttcatatcat ggtagtttaa aatcgagtag tataatagta gatacagaat
     4801 acaatataaa aatttgtgat tttggaatta caaaagcaaa taatggggtt aatacaagaa
     4861 gctatggaaa caggagatat ttatgcccac atcaactatg tataaattat actgataaag
     4921 aaagtgattt ttttgcaaca ggtcttattc tatttgaatc tatatttaaa aaattgcctt
     4981 ttggagaatc aaattcagaa gaaaaaatgc tacaatctat tgataaggga ttagattgga
     5041 atagtataaa agcaattaat ggaaatactg aacttataaa tgttataaaa aggcttcttg
     5101 gaagaaataa taagtatacc agagccaatg atattattat tgatttaagt aaagtaatgt
     5161 atgaaaaagc atatatagaa gttgaagatg agaaagaaga agagcagaaa caagacaaag
     5221 ttatacaagt taaaaaagaa tataaaagta aaatgagaaa aaaattacat aaaaaacttg
     5281 ttgttgccgg tctagctata gtcatgattt caatgattat tattagttcc ataatataaa
     5341 aattgtatat taagtatttt aaaatcaatg tctataaagg atatacatag aaattttagt
     5401 gtagtcttaa attatgaaag gaagtggtta ttatgttaac aaaaaataat gaggtattaa
     5461 acgtctagga gtattttaac aaatagccac ctgcttttac agtttagaat aaaaaagctg
     5521 tggctaaagg tcacagcttt ttgctacttt ttaaaggcag gagaatatta aaaaggagca
     5581 aattatgagc aatataaaaa taatagcaag taataaatgt tggattgaag atatagcaag
     5641 gaaacaactt gaagatattt caaagttaaa tggaatttta agagtagtag gattacctga
     5701 cttacatgca ggtaaaatac ctgtaggttt agcagttgag actaaaaata ttatttatcc
     5761 acatataatt ggaaatgata taggatgcgg aatgacttta tttaaaactg gagttctaaa
     5821 aaagaaattt aaaaaagaca aatggattaa gtcacttagt aagattaaag atttgtctga
     5881 tatagaaata aaaaacacat ataaagaaga atgtccaatt ttaaatttag gaactatagg
     5941 gagtggaaat cattttgtag aaattcaatg tataagtgaa atttataata aagaacaatt
     6001 tgaacaatta aaattttcat ctgatgatat tatgatgtta gttcactgtg ggtcaagaaa
     6061 ttatggagaa gatattttaa aaaaatttta tgataaagat ggacttgaag ttgaaagtga
     6121 gaaagctata gattatatta aaaaacatga taatgcttta atgtgggcag agcgtaatcg
     6181 tgaagctatc agtaaaaaaa ttatgcagtc aataggtaca tctggagatg ttgaaacaat
     6241 attctctata aatcataact ttgtagagaa aagggaagat aaatttatac atagaaaagg
     6301 tgctgtttca agtgaaagag gagctgtgat tataccaggt tcaagaggca gtctatcata
     6361 tatagttatg cctactgaaa atacagagat atctttatat tctctatcac atggagcagg
     6421 taggaaatgg tctcgttcag tttgtaaatc tcgccttaaa agtaagtata gtaaagatac
     6481 tataaaacaa actaaattta aaagtcagat tatatgtaat gatgtaaatt tattatttca
     6541 agaagctcca gaggcatata agaatataga gcagattatt gaaagtttga tagaatatga
     6601 acttattcaa gtagtggcaa ctatgaagcc attgattact tataaaggat aatttttaaa
     6661 gttaaaaaag attaaaatta tattataaat agctaatttt atggaacatt taaaagtgtt
     6721 ttattattag ctattttttt attttgtaga gacgataatt tatagagcta aattctttga
     6781 tatataatga ttacaaagtt agactgtatt ggaaactgca catacatgaa ttgatgacaa
     6841 tataagtgat tccaaaatat aaatgaaagt ataagcttat tttcactata aaaattttca
     6901 ctgtacaaaa acattaatga cattattttt attgacataa atatataatt tatggaataa
     6961 tattagaagc actgatataa gtgtttaaat aaggatagaa aaggagaagt gttttatgaa
     7021 aaaaaatact aacattaatg agtccggagt taaacttctt aataaataag gagtttgata
     7081 tataataact ccttatttat taagttgttc gcaaaataac tattatcaaa gtaaatgata
     7141 gggtgattta aaagacgata acttataaat aaaggagaaa aaataatgaa tagtaataca
     7201 tttgaaagat tacaactaaa tgaagttaaa gaattaataa aaatacattg tgtaagttca
     7261 ctaggaaaaa atttaataga taaattaact cctagtggga atattggagt agtaagaagg
     7321 aaattagcag aaaataaaga agctagaaag ataattgaaa atagtaatca cataccactt
     7381 gaaggattgt ttaatgcagg ttctacaata gataaaattg aaaaaggaat gataatagaa
     7441 cctgttgaac ttgtaaatat tgaagatttt ttaagaggtt gtaggaagat gaaagctttt
     7501 atgttagaaa aagaatttta ttcacctaca ttaagttctt atgccttaaa tataacagaa
     7561 tgtaaaagta tagaagatga gataaattac tgtataaaat ctaataaagt tgattctaat
     7621 gctagtaaag aattaaaaaa gataagacga aatatagaaa ttacagaagg aaagataaaa
     7681 gatagattaa ataaatttat aacatcaact gtaaataaaa agtatataca agagtttata
     7741 ataagtaaaa gaaatgatag atatgtaata ccaattaaat cttcttataa aaatgaagtt
     7801 aatggtacta tattagatac atcatcaaaa ggaaatactg tatttattga acctattagt
     7861 gtttcaaatt taagtacaga gttaactatg ctaaaagctg atgaaactat tgaagaatac
     7921 aaaatattat cttatttaac agaacttata tttgagaaga taagtcagat aaaattaaat
     7981 atagagatac tttcagaata cgatatgtta tttgcaaaag ctaagtatag tcaaaaaata
     8041 aaaggaatta ctccaaaaat aaataataat ggctatataa aaataattaa aggtaaacat
     8101 ccacttctaa caggagatgc agtaccttta gattttgaaa taggcaaaaa ttatagaagt
     8161 cttataataa caggtcctaa tgcaggagga aaaactgtaa cccttaagac tgttggttta
     8221 ctaactttaa tggttcaatg tggatttgat atttctgcaa aagaaggtag tgaatttagt
     8281 gtatttgaaa aagtttttgt agatataggg gataatcaaa gtattgaaaa tgccttaagt
     8341 actttttcat cacatataaa aaatattgca gagattatga gtttatcaaa taattctact
     8401 cttgttctgt ttgatgaaat aggaagtgga actgaaccta atgaaggagc tggtttagca
     8461 atatcattat tagaagagtt ttacaaaatg ggatgtataa ctatagcatc aactcattat
     8521 ggagagataa aaaagtttgc tacattgcat ccagaatttg aaaatgctgg tatgatgttt
     8581 gataaagaaa cgttagaacc attgtataag ttaactattg gaaaatctga agatagtaat
     8641 gctcttttta tttctaaaaa aatgggaata aaaaatagag ttttagatag agctaaagaa
     8701 tatgtaattg atagaaacta caatttagat ttagttaaaa gaagtaagct acaatctaat
     8761 attgaagata aaaatagttc tgaagtcatt ttaaactata cagattataa tgtaggggat
     8821 aaggtaaaat tactagatga agatgatttt ggaattgtat ataagcctat ggacaagttt
     8881 aataatgtag aagtattgtt taatgataat tttgtttcag taaatataaa aagattacaa
     8941 cttagcataa aagcatcaga attgtatcca gaagggtatg atttagatgt attatttact
     9001 agttttaaag aaagaaaatt agaaaaagat attcaaagag gttcaaaaaa agctcttaaa
     9061 aagattcaga aagaaattag agagaataga aaataaatat aaaaagcaca aaagaaagca
     9121 atctaaagag aaaagtttat ctttagattg ctttcttatt aacatatctt cataaatcca
     9181 atgactaaca agattaaacc acttaaccaa gataagttta aatttacgtt tttaacaaac
     9241 atatttccta gtaaacaacc aagtaatact gcaattatat ttgatattaa agaaaataga
     9301 ataacttgta tataattcac acctacaagg ccactaccaa aaccgatagc taatccatct
     9361 aaacttaaag caagtgcaag tgtaaatgct tcagcagatt taagcacttt agatttactt
     9421 gtatctaaga catcaggttc gatacttaca ttaaaattta atttaaggtc taacatatta
     9481 aaattataat tctttgagtg tatagatttt ttcttaaaat aagatttaaa aaaactttca
     9541 attagtctag cacttcctaa taaaaataaa ataaagaaac atattaacat tgttatattt
     9601 tgaggtaaaa aatctctaac tattgtacct gcaaaaagcg atattgctaa aatagctgaa
     9661 caaactacgc tgataattat atttgaatat aagggaactt taactttatt tacaccatat
     9721 ccaaagcttg tcacaaaggc atctatacaa agagaagtaa ctaataaaat tgattcaatc
     9781 acagtagatt aacatccttt ctcttaaatt ttgtacttat attagttata gtattgtaaa
     9841 aatggataaa ttgttacaga taatccaata aattgttgat aaaattccaa catcatacaa
     9901 cacctaaata acaaaagaat catatcatat aataatataa aaccaatcat aataaagcgg
     9961 gtgaaaataa tggaacaaaa taataaggtt gggaatttcg gtgatttgtt gaagatttta
    10021 ttagatgaaa aagaattatc aatgggagaa ctaagtaaaa aaagtggaat tgataaatct
    10081 actatatctc gtatagctaa taataaacaa aagccaaata taaatcattt agaaaagatg
    10141 gcaatacatt taaatattaa cctagaagaa ctgttgaaag cttcaggata tgaatttaga
    10201 aacagcaata atcaaatatt taatgcagat agtgatttta gtaactttga tgatattctt
    10261 ggatttgcaa atttaatcaa tgataagaac tttaatggaa atattgaaaa agagttaagt
    10321 aagtgtaagt tatatgtcca aacagatgaa ggtaaaaaat tactttttga taattttaat
    10381 aaaaaaatag attctattga aaaacaaggt caatttacag atagattaag acaaatgtat
    10441 gctgattttt gtacagatgg acttacaata aagaaatatt tattgattgg aagtatgcta
    10501 ctttactttg ttatatcaac agatgtgatt ccagacttcg tgttcccaat aggatttatg
    10561 gatgatttag tagcattaaa tatagttaca aaattactta aaaatgataa gtaagtttta
    10621 gaatcatatt taatatataa ataaat
//
LOCUS       DAEOSP010000135        10466 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.137, whole genome shotgun sequence.
ACCESSION   DAEOSP010000135 DAEOSP010000000
VERSION     DAEOSP010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10466
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.137"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            103..3000
                     /locus_tag="KRH54_002617"
     CDS             103..3000
                     /locus_tag="KRH54_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG8580261.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEKFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            3321..3533
                     /locus_tag="KRH54_002618"
     CDS             3321..3533
                     /locus_tag="KRH54_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG8580262.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            3546..3767
                     /locus_tag="KRH54_002619"
     CDS             3546..3767
                     /locus_tag="KRH54_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG8580263.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            3867..6023
                     /gene="feoB"
                     /locus_tag="KRH54_002620"
     CDS             3867..6023
                     /gene="feoB"
                     /locus_tag="KRH54_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG8580264.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            6049..6201
                     /locus_tag="KRH54_002621"
     CDS             6049..6201
                     /locus_tag="KRH54_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBG8580265.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            6381..7091
                     /locus_tag="KRH54_002622"
     CDS             6381..7091
                     /locus_tag="KRH54_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG8580266.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            7176..7946
                     /locus_tag="KRH54_002623"
     CDS             7176..7946
                     /locus_tag="KRH54_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8580267.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGANKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            7939..8670
                     /locus_tag="KRH54_002624"
     CDS             7939..8670
                     /locus_tag="KRH54_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580268.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNVYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            8704..9663
                     /locus_tag="KRH54_002625"
     CDS             8704..9663
                     /locus_tag="KRH54_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG8580269.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(9761..10357)
                     /locus_tag="KRH54_002626"
     CDS             complement(9761..10357)
                     /locus_tag="KRH54_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBG8580270.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
BASE COUNT         4028 a         1097 c         1886 g         3455 t
ORIGIN      
        1 tgagaatatt gcaatttgat atatttacca ttacaataaa agtagttaca actagaaaac
       61 caaataagtt aaagtaagtt ggcaaattgg aggtttttaa atatgaaagc atttaaaaag
      121 cacttcttga tactttcttt gatattttta cttattatat catgttttaa caatatacta
      181 tgctttgctg atgagtctga aaattcaaaa aaaataagag ttggatattg tgatgactac
      241 ggaataatat cctcatcaaa agagcattct tatacaggat atggttatga ttatctaaga
      301 gaaatctcaa aatatacaag atgggaatat gaatttgtaa aagggtcttg ggaagaatgt
      361 ttagacagac tagaaaaggg tgaaatagac ttactcggac ctttacagaa gaatgatgaa
      421 agaaataaac tatttaattt tcctaaacta agttcaggat atgaatatag tgcactatat
      481 acaaaggata gtaacaataa tatgttttat gaagatataa gttcctttaa gggaatgaga
      541 gtagctgtac ttagaggaaa ctttcataat actgcatttg agaagtatag agaagaaaat
      601 aatttttcag ttgaatatat atactgtaat tctattgatg aattaattga gtccgtcaat
      661 gaaaaaaagg cagatgcatt tgtttgtgga agtattataa atgctaaagg aatgaaaata
      721 gttagcaaat tttcagtaga acctttttat tttgctactg caaaagataa acctaattta
      781 gtaaaagaat tagattatgc tttaaaagaa ttaaaaataa atgatatgta ttatgagttg
      841 gaattatata aaaaatattt caaaagagag gtcaataatt ccatagcatt tactcggcaa
      901 gaaatgaatt ttattaaagc aaacccaaaa cttactatgg tatatgattc agagtggtct
      961 cctgtagagt attatgataa agaaagcaat tcatttaaag gtataagttc cgatttaatg
     1021 tcaattatat caaaaaaatg tggtataaaa tttgaatata ttaaaacaaa aaattataat
     1081 gagagtcttg attacataaa aagtggtaag gcaactatgc tatgtggctc aatcaatgaa
     1141 aatgataagg caaaacgatt taatatgaaa ttgacaaatc catacataaa tattccaatg
     1201 attatggtag gaaaattgga taccaatctc aataatgatt taaatattgc tttaactagt
     1261 agttataagt ctattgattc ttatatagaa aagagttttg aaaatgctaa gacaacatct
     1321 tataagagtg tagaaagttg tctaaacgct ataaatgaag gtaaagcaaa cttaatgata
     1381 cttagctctt atcagtttga tgaactttta agagaaggaa agagtgagaa tttatctgtt
     1441 atatctgttt tagatacaag ctatgggatg cgtatagggg tatcaaataa aactgaccct
     1501 atattagtgt ctattttaaa taaatcaata gacaaaatta ctgaagaaga attaagtgat
     1561 tgtatatatt ctaatactat tgacaaacct tataaagtac catttggtgt gatttttaaa
     1621 gaatattcaa tacaaattat ttcttttgta tgtattttat tcttaattgc tattaaatat
     1681 ataatttata ataaaaagaa gaaagaggat tatctaagga agatagcata tacagaccca
     1741 ttgacaggag cagatagtat agataaattt aaaataaatt ctaataaact gtttgctaaa
     1801 aataatccag aagaatatgc gttgttttat atagatgttg acaaatttaa atatataaat
     1861 gatatgtttg gatatgatat gggaaatgat actttgatac atatatcaaa tactattgca
     1921 agtgaattaa aggaagatga aatttttgca agagtttctg ctgaccattt tgttcttctt
     1981 attaagtata agacagatga tgatattaaa acaaggttaa acaatattta caataaggtt
     2041 caaatactta gtaatcctaa aataaactat tataaattga ttttagattg tggtatatat
     2101 aaaataagta aaagtgataa tgacataaat actattatgg atagagctaa tactgcaaga
     2161 aaaactataa aaggtggtca taaaaattca tttgcttttt acgataagga aatgcacaaa
     2221 aaaatattaa aagagaaaga aattgaaaat tcaatggtag atgctcttaa taatggagaa
     2281 tttattgttt attttcaacc taaatatagt ctttcagatt atcaaattat aggggcagag
     2341 gctcttgttc gttgggataa tccacaaaaa ggtctaatac caccaataga atttattcct
     2401 gtatttgaga ggaatggttt tattgtaaat attgatttct atgtatttga ggaagtgtgt
     2461 aaaaagatta gagaatggat ggatgaagga caaaaagtag tacctatatc tgtaaacttg
     2521 tctcgaatgc attttgtaaa tagtaatttt atagagaagt ttaaattaat tgtagataaa
     2581 tacaaaattc cgacaagact tatagaatta gagctaacag aaacagcagt attagataat
     2641 atagaaggat tacttgatac aatgaacaat ttgaaagaaa aggggtttgt aatctctatg
     2701 gacgattttg gtacaggata ttcctctctt aatcttttaa aagaattgcc tgttgatata
     2761 ttaaaactag atagagcatt ttttacagaa aaagatgaga gtaataatga aaaaattgtt
     2821 atatctaatg tcattaaaat ggcaaaggaa ctgaaaatga aagtaatatc agaaggtgta
     2881 gagactattt cgcaagtaga atttctaaag cagattgggt gtgatatggt acaaggatat
     2941 ttgttttcta agccaatgcc tgtaaaagag tttgaaaaaa tcgcttttaa aaaagaataa
     3001 agcaatgtgt aaataatgac atatataata aaggtgcttt aatattagtt gagtatacta
     3061 ttttagagca tctttatttt tgtattaatt tttacgattt tattaataaa ataaatcaaa
     3121 tgagaaaaaa aatcaaaaga atacaataat cacttgcaaa aaatatacta ttgtgataga
     3181 cttaaatcaa aattaggaat tgtaaaaaga aggaaaaaat aggatattat tttaatagat
     3241 taagatttaa ggtattgtaa tttatatata agataacgat tgcttgtaaa tttaaattac
     3301 tttaagagga gggatattat atgatgccat tgatattatc aaatacaggt gaagaagtag
     3361 ttataaagaa aatttcggga agtgataaga cgaggacttt tttaaatagt ataggttttg
     3421 tagtgggtac aaatgttaaa atagtttcta agatagatgg taacttaata atagacgtaa
     3481 aagatactag agttgcatta gataaaaaaa tggcaagtag aataataata tagggaggta
     3541 atgacatgaa cagactgaag gatattaagt gtggagagac tgtaaaagtc aaaaagttag
     3601 aaggagaagg agctacacga agacgtataa tggatatggg gattacaaga ggtgttaata
     3661 tatttataag aaaagtagct cctcttggtg acccaattga ggtaacagtc agagattatg
     3721 aactatcaat tcgtaagtca gatgcagaaa aaattattgt ggaataataa agattaatta
     3781 gagtagggtt aatgtaatca tataatgaga atgattttct gtatcacaaa cagtgatgta
     3841 tttaaataat aatggaggga atggaaatgt caataaaaat aggtctcata ggaaatccta
     3901 actgtggtaa gacaacaatg ttcaatgggc ttacaggctc atcacaatat gttggtaact
     3961 ggccaggagt aacagttgaa aaaaaaggtg gaaaattaaa aggaaataaa gatgtggaaa
     4021 tagtagattt accaggtatt tattctttat ctccatatac attagaagaa gtagttacac
     4081 gtaattttat gctagatgat aagccggatg cagtaataaa tattgtagat gcatcaaata
     4141 tagagagaaa tttatatctt acaactcaag ttttagagct aggtataccg accgttatag
     4201 ctcttaatat gatggatatt gtaaataaga atggtgataa gataaatatt aaagaactat
     4261 ctgaagttat aggttgccca gtggtagaag taacagcagt taaaggtcaa ggaattatgg
     4321 aagctgctga aaaggcagtt gaacttgcaa gtagtaacaa taaattaaat ttcaaattac
     4381 cctttgttga tgaaagtaag gatgcaatag aaaaaattga aaaaataata gaagaaaaga
     4441 ctccatatat agatgtagaa acacgctggt tagctataaa gctatttgag agagatgaaa
     4501 atgttataca aaaacttgat atttctaaaa caatattgaa tagtattgaa gaaataacta
     4561 gaaattgtga agatgaattg gatgatgata gtgaaagtat aataacagcc aatcgttatg
     4621 aatttattag ttcaataatt tcaagtataa taaagaagaa tcgaaaaggt aaggaaactg
     4681 tatcagataa gatagataag attgtaacaa atcgtatact agcattacct atttttgcac
     4741 ttataatgtg gggagtttac tatatagcag taagttcatt gggtactata gcaactgatt
     4801 ggacaaatga tgtactgttt ggagaaatta ttcaaggcaa tgtaagtaat tttctagctt
     4861 ctttaaatgt agcagagtgg ttacaaggac ttgtagttga tggtctaatt ggtggagttg
     4921 gagcagtact aggatttgta ccacaaatca tgcttttatt cttattatta tcaatattag
     4981 aagattgtgg gtacatgtca cgtgtagctt ttataatgga tagaattttc cgtaagtttg
     5041 gtctttcagg aaaatcattt ataccaatgc ttataagttc aggttgtgga gttccaggag
     5101 ttatgtcaac tcgtactata gagaatgata gagatagaaa aatgactatt atgttaacta
     5161 cattcattcc atgtggagct aagattccaa taatagcatt atttgcaggg gcattatttg
     5221 gaggtgcttc atgggtagct ccatcaatgt atttcttagg tattgcaatg ataatcattt
     5281 gtggaattat attaaagaaa actagtttat ttgcaggaga accatctcca tttgttatgg
     5341 aattacctca atatcatatt ccaagtgcaa aaggtgtttt aatacatatg tgggatagag
     5401 gaaaagcatt tataatcaaa gcaggaacaa ttatatttgt tgcttgtgga gtaatctggt
     5461 tcttacaatc atttaattgg tcattacaaa tggtagatgc tggagatagt atattagcta
     5521 gcttaggaaa tatagttgca ccaatatttg ctcctcttgg atttggaaat tggcaatcat
     5581 cagtagcaac agttacaggt ctagtagcta aggaaaatgt agttggaaca tttggagttt
     5641 tatttggtat atcagatgca acagagcaag acccaacact tcttgcttca gttgcaagta
     5701 tgtttacagt agctagtgcg tttgcattta tggcatttaa tatgctttgt gcaccttgtt
     5761 ttgcagctat tggagccata aaaagagaaa tgggttcatg gaaatggaca tggattacat
     5821 taggattcca aactttaaca gcgtatataa ttgctcttct tataaatcaa gttggaagtt
     5881 tagttttagg tactggagga agtattgctg gggcaataat atctatattt atagcagtag
     5941 cagtagtctt tgtagtttta acttattcaa ataaaaatat gaaaaaagaa aaaatgggta
     6001 aattaagtta tatgaaaaat taattcatat aaataaggag tgatgaatat ggctacattt
     6061 attatagcag ctattgtagt agtattaatg gcactagctg taatgtatat ggttaaaaat
     6121 tcaaaaaaga atggtagcag ttgtggatgt ggttgttctg gatgctcaag tagtaagagt
     6181 tgtcatagcg ctaagagata aataggataa gagcctgata aaaagttaaa ttgctttatt
     6241 atcaggttct tattttttgt ataaataaca aatttcatat ttgaagtagg ttagactatg
     6301 ctgtaatttt actgtataat tatatataaa tatatgatta ggagggatta ttgctaaatt
     6361 aagcaatata agtgaatatt atgtttaaat ttgatgttac aaacgagctt atgtgtatag
     6421 caaggggaag tggtaaattt tttgcaaaaa aaggtgctat ggtagccttt aaaggaaatt
     6481 ttaattttga aaaattgctg ttaggtccaa gtaatggcgg tggattggga agagcattat
     6541 taggtcatgt tcagaggtca ttgacaggag aacaaatgcc aataatggtg gttgaaggtg
     6601 aaggtgaaat ttacctagct caaaatgctt accatgtaga tgtaatatca attgacccag
     6661 gtgattccat ttatgttgag agtgaaaact tattggcatt ttctgaacaa ttaaattaca
     6721 gtgtaaaatt gattggttca ggggtaatat ctcaaaaagg tctttttact acacatttag
     6781 taaataaaac aggtcaaaat caagatgttg ctataataac agatggtaat cctctaattc
     6841 ttgaaggacc ttgttgtgtt gacccagatg cattagtagc ttggactgga agagaaccat
     6901 atcctaaagt atcaaaacta tcatggaaga cttttattgg tcagacttca ggagaatctt
     6961 atcatatgga atttgttgaa cctggacaaa tagtcattat acaaccatct gaaagattgt
     7021 ctggcttaaa aacagattct acaccttctg atagaatgag ttcaggtgga ttaagacttg
     7081 gcatagatta aatcttatga tatagcttaa ttattattta agatattaaa aaataaaaag
     7141 ggtagcaggg atagatatgt taaaaggagt tttatatgtt tgagaaaatt cataaaaggc
     7201 ttaaaggata ttatattttt ttaataattt taattttatg gaagatagta tgtgttttgg
     7261 gtatatggag tagctatatt ctgcctcctc ctgaaatagt cttagatact tttataaaaa
     7321 tgatatatga cggttctatt ttcttaaatg tttatgccag tgtaagaagg atattaatag
     7381 gttttttaat aagttcattg atggctatac cacttggagt tttatttgga gcaaataaaa
     7441 aaatgtatga atatttcaaa ccactatttg aatttatgcg aagtacacct ccactagctt
     7501 tggtgccaat gcttatttta tggtttggta taggtgaaga atctaaaatt ataattatta
     7561 ttttggcatc cttttttcca atatttttaa atacattgaa aggtataaaa aattgtgaca
     7621 ataaattaat agaagtaggt aaatctttta atttatctaa aaagagaatt ttttataaaa
     7681 taataatccc taattctatg ctggatattg ttgttggact tagtttaggg cttgggtata
     7741 gttttagggc tataattgga gcagagctta ttgcagcttc ttcaggacta ggatatttga
     7801 tttctgatgg aaaagatatg tctagaactg atgttgtatt ggtaggaatt tttgttattg
     7861 gatttttagg tatactttca gattatctat tttcaaagat aataacaaaa tttagcaaag
     7921 ggaagcaggt tgatttaaat ggttagaagt ggatttttgc tagagaatat atataagaaa
     7981 tatttagttg ataataaaga gcatttagtt cttgataata tatctttaaa tatatctagt
     8041 gaagagataa ctgttatatt aggtgaaagt ggatgtggga aaacaacgct acttagaatt
     8101 ttagcagggc ttgaaaatgc tactagtgga aacgtatatt tttttaataa tgataaaaag
     8161 tgtactccaa aagtaggaat ggtatttcaa gaaagtagac ttatgccatg gctgaatgta
     8221 agtgaaaata tacttttaca tactgaaaaa gataatagaa ataaagttga tttagacaag
     8281 tatttaaaaa tgatgaagct tgaaaagttt aaaaactcat atccaaatga attatcagga
     8341 ggaatggctc acagagtaag tatagcaaga gcactttcct tcaatcccga tattttgctg
     8401 atggacgaac catttgcagc actagattac ttcacaagaa gaaaaatgca aaaagaagtt
     8461 gtaaatatcc ataaaaatac taaaaaaggt gtagtatttg taactcacaa tattgaagaa
     8521 gctatggaaa tagcaaagaa aataattgtt tttagtaaaa ataaaaggat taaacaattt
     8581 agtgtcgaag atgaatataa tagagattta actaaaaact attatataaa cttgaaaaaa
     8641 gaaatattaa gggaattggg ggagttttaa ttgattaaaa gaaacaaaaa gattttagca
     8701 gtaatgacat ctttaataat ggtgttggtt gggactgtag gatgttcaac aacaaatgat
     8761 aagaaagatg aacctgcaaa aaaagaggct agtttaccta aggaaataaa tttaacttat
     8821 gtaaaatcac cacttaatgt tcctagtata ttagaaaaaa aggatgattt atttggaaag
     8881 gaatttaaaa aggacaatat aaaagtcaat ttccatgaat tgacaacagg accagagcaa
     8941 actcaagcat tagcagcagg tgagctagac tttttacatg cattaggagg aacgtctgca
     9001 ataatagctg catcaaatgg tgttggtctt aaaattacga atatttatag ccgttcacca
     9061 aaaggatata tgcttataac taataatgat aatattaaat ctataaaaga cttaaaaggt
     9121 aaaaaaattg taggtccaaa agggactata cttcatcaac tgttgattgc tgcacttgct
     9181 aaagaaggct atactataga tgatgttgaa tatgtaaata tggatattcc tagtgctgct
     9241 tctgcattag aaagtaaaag tgctgatgta gcaatgcttg caggtccagt ggcattgaaa
     9301 acgataaatt caggtgccaa aatgattgta aatggagaag gattagtaag tggaataata
     9361 gttactgctg taagtgatga ctttgctgaa aaatatcctg aattagtaaa aagatttatg
     9421 aaggttcatg aagaaacttt aaaatatatg aatgaaaaca aggatgaagt gatggatgtt
     9481 gtatctaaag aagttggttt atctttagat gaaacaaaag aaatgtattc ttggtatgac
     9541 tttagttcta aaataacgga caaggatata aaagaattag aagatacaca ggaatttttg
     9601 atgtctaatg gaatgcaaca aaagaaaata aatattaaag atatgttgta caatcaaaat
     9661 tagaatactt taaaatatta aagtggaaaa tacaccctta tttattatgg aaactgtatg
     9721 gtaaaagagg gtgtattttt attattatac aaaaacacaa ttaaaaacag cttactattt
     9781 tatctacact tatgtagtct agtaattttc tcattttctt acggtgaagt aaaaacttcg
     9841 aatatgtgtc ctcggattgt tttgtgtcac cataaacttt ccaatattta atcatagaaa
     9901 cttcataatt actatatccc ataaatccaa tcacatcttt aagaggatat cctaaaaaaa
     9961 tacctatttc atcaggaaaa acatcactct taagtttatc tactaaatgc tctaaataag
    10021 cattaacatt cgtattttgt ttgtatccta aaaactttaa aaagtttact gtttttttac
    10081 attttaattt ttctgataat gcttttttat ttacaaataa gatttttaat cccttttttt
    10141 ctttatcaat aactataaaa tctattttct tacacttata tagatattta taaatatcat
    10201 taattttttc ctctctattt acatcagtaa acgaaatatt tattatttca gcaggtttag
    10261 agcctaaaaa gactggacct aaaacttcta aaagccattt tatatatgat gattcaattt
    10321 tatttttaca acaaaattgt gaattacaac aagacataaa agataacctc cttcaaatga
    10381 aaattaatat caagtaatta gattatacta tgaatgataa tcaatatcaa tgataaaatt
    10441 ataatttttt tatttaatta tttatt
//
LOCUS       DAEOSP010000136        10458 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.138, whole genome shotgun sequence.
ACCESSION   DAEOSP010000136 DAEOSP010000000
VERSION     DAEOSP010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.138"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            75..917
                     /locus_tag="KRH54_002627"
     CDS             75..917
                     /locus_tag="KRH54_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG8580271.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            932..1396
                     /locus_tag="KRH54_002628"
     CDS             932..1396
                     /locus_tag="KRH54_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580272.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            1383..2555
                     /locus_tag="KRH54_002629"
     CDS             1383..2555
                     /locus_tag="KRH54_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG8580273.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            2599..3534
                     /locus_tag="KRH54_002630"
     CDS             2599..3534
                     /locus_tag="KRH54_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBG8580274.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(3905..4630)
                     /locus_tag="KRH54_002631"
     CDS             complement(3905..4630)
                     /locus_tag="KRH54_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG8580275.1"
                     /translation="MLESKIRTPEEIKRYMLDLKKWLAQERSKPVEEMSDFFSKRIDI
                     YDNVHLANWTEEYAHIADYFDTPLSSLLDIGCGTGLELASIYRKFPNVQITGIDLSKE
                     MMSKLKEKYSDKNIDLILADYFEYPFEQEKYDAALSFETLHHFKYQKKQKIYKKLFQT
                     IKPGGYYVECDYIACCLEEETICLEQYEFKCKENHVPDDVFVHIDIPLTIEHQIHLMK
                     NAGFRAVYVLHENNGTMIIKAEK"
     gene            complement(4648..5214)
                     /locus_tag="KRH54_002632"
     CDS             complement(4648..5214)
                     /locus_tag="KRH54_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8580276.1"
                     /translation="MPPKPIITKQDIINAAIQLVRENGISSVNARNLAKTLNCSTKPL
                     FRIYTNMDEIKEDIKIELDNYYNSFMESKINDENRLLSQGLAYIEFAQNEKMIFNTLF
                     MNMTMKGSSLQDIIHAKWNRITIENAKKVTGLSIEKSEMLFINFWLYSHGVATQIISN
                     GIDIPLDMVQQLLENAFERFSLDITKTE"
     gene            complement(6125..6523)
                     /locus_tag="KRH54_002633"
     CDS             complement(6125..6523)
                     /locus_tag="KRH54_002633"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBG8580277.1"
                     /translation="MKWKELYNKARGKLNPRTISPFIDAGGVAAAILTDSGNVYTGVC
                     IDTACTLGMCAERNAIANMITNGESKITKLVCVMSNGSVGSPCGACREYLMQLDKDSA
                     CIEILVNVETEKTVKLSELLPDWWGSDRFI"
     gene            complement(6822..9515)
                     /locus_tag="KRH54_002634"
     CDS             complement(6822..9515)
                     /locus_tag="KRH54_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG8580278.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVEPEVAYVVTDMLESVITEGTGGVAALPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     SATPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGSGTPAT"
     gene            9729..10427
                     /gene="yunB"
                     /locus_tag="KRH54_002635"
     CDS             9729..10427
                     /gene="yunB"
                     /locus_tag="KRH54_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBG8580279.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
BASE COUNT         3516 a         1627 c         1774 g         3541 t
ORIGIN      
        1 atgataggtt atataataaa atcactaaca attatattat aagaaaatta taaaaattac
       61 aaggaggtat agaaatgaaa gaaaaagcag ggattagaaa tgccataata gcagccttta
      121 taatcacaat attggcagaa ttaattggac ctatttcatt taaagttatg ggaattaatg
      181 taactttact tagtatacta tgggctatat tcataggaat ggctgtttct cctcatttat
      241 tagggagagt tataccagcc ttaaaaaagt ttatagggga taatgaaata aatgtatctc
      301 catatttatt atctttgaca ctatatccat tagggataat gtttggaatt aatgcagggc
      361 ctaaagttgg tatattactt caagcaggac cagcactttt attacaagaa tttggtcata
      421 tgggtacaat gttgatagca ttacctgttg gagtagcatt aggtcttgga agaagtgcat
      481 taggtggtac tttttcatta tgtcgtgaca cagcactagg aattattgga gataagtatg
      541 gattaaactc accagaaggt ataggaacac taggaactta cataagtggt agtatatttg
      601 gaacattatt attttcattt ttagcaccag tgggtgtact tttaggattt catccatatg
      661 ctttagcaat ggcatcagga atgggtagtg gttcaatgat gggagctgca acagcatcat
      721 taatgaatac ggttccacaa atgtcagagc agattttagc atattcagct actagtggat
      781 tgattacagc tgttactgga atatatatag aattattctt atctttacca gtagccaact
      841 actattatag caaagtagct ccagttattg aaagatttag aggaagaaaa acaagaagaa
      901 caagtaataa attgtaaagg aggatatata tatggaaaaa acttttaaga ctattatgga
      961 gcagataaaa atagtattgt ttatatatat tattatttgc tttggacaaa tgatagcact
     1021 taagactcag gctcaagatt tgttgattgg aagtgtatct ggatttttgt tggtgataat
     1081 tgctattata ataaagaatt ttgttaaaaa acctaattta ccaggttttg catgggctac
     1141 attagttgca ttcgttttat cgcttccaat atctccagtg ggggatatca tagcaacaaa
     1201 tgtagggaaa atcaatttta tgtctacagt aacaccatta ttagcttttg caggaatctc
     1261 tgtaggtaac caattagata tattaaagac tttgagttgg aagttagtag ttatatcatt
     1321 tatagttatg acatctactt actttggttc tgcttttata tctcaaacag ttttaagatt
     1381 aaatggaatg ctttaaggag gtagtaaata tgaatgaatt aaaagcaaaa attaaagagg
     1441 aaataaagtt attatctgaa gagaaaaaga catcatttga aaaagtttct gactatttat
     1501 tttcaaatcc agaattagca tttgaggagt ataagtcaca aaaagcatta tgtgatttgt
     1561 tagaagaaaa tgaatttaat gtaactaaag gtgtaggagg attagaaaca tcttttgaag
     1621 ctgtgtattc aaatggaact aatgggaaaa cagttgcttt tttagctgaa tatgatgcac
     1681 ttccaggaat gggacatgca tgtgggcaca atatcatagg gacaagttct gttggtgcag
     1741 gtatcatatt aaaagaaatc atgaaaaaac ataatataga agggactgta aaggtttttg
     1801 gaacacctgc agaagaaaga gtaggtggaa aaataactat gattaaagaa ggtgtattta
     1861 ataatgtaga tgctgcatta attttacatc caagtgatgc ttcaatgcct gatgatatat
     1921 catttgctca agttaattta aaatttgatt ttactggtaa ggcatcacat gctgctgctt
     1981 ttccttggga agggaaaagt gccttaagtg gagtaatagc actatttaac agtgtaaata
     2041 gcatgagatt acatcttaaa gattatgcaa gagtacatgg aattataact gatggaggta
     2101 gcatacataa tataattcct gaaaaatcta cagctatatt taatgtaaga gccttaagta
     2161 tagaatattt aaatgagatt tgtgaaatgc ttaaaaattg tgctaaagga gctgcaattt
     2221 ctactggaac taatgtagaa atagttcaat tagatgaaat atacaaagag attaagaatg
     2281 attctgaatt agtgaatata gttagacaaa attttgaagt tttgggtgaa gactatgttg
     2341 aaagagattt aagtcaaggt ataggttcaa ctgatacagg aaatttaact catgagatac
     2401 ctgcaataca agcatatata aaattaaaag aaaatactgc aactcataca gatgaatttg
     2461 ctgttgcagc tggtggtgaa gaaggcagag tagcacttat aaaagcaatt aaggtgttag
     2521 caatgtgtgg agtagatatt ttgtatagta aataatatat aattttaaaa tatattaaga
     2581 aaggaaatcg gattagacat gatattaaaa caagtaatag aggtttatga cattttagat
     2641 aaagcaaatg ctaatgggga agaagtaaaa acttacttac aaggttatgg tgaagtagat
     2701 gtaactgtaa aagaattgtc ttcaagtaaa ggctcaacag atttagtaaa gcttacaatt
     2761 cctgggaaaa atggaaaatt aaaaggtgga gatgctccta ctcttggcat tttaggtaga
     2821 ctaggaggga ttggagcaag accagaagtt ataggttttg tttcagatgg tgatggagca
     2881 ttggtagcaa ttgcagtggc tgctaaactt ttggatatgc aacgaaaagg ggatatatta
     2941 gatggtgatg ttgtaatttc aacccatatt tgcccagatg ctccaactaa agaacactat
     3001 cctactcctt ttatggattc tccaattgat atgatgacta tgaatgaaaa tgaagttgat
     3061 agtagttgtg atgcaatact ttctatagat actactaaag gaaatagagt aatcaatact
     3121 agaggatttg caatatcgcc aactgttaaa gaaggctaca tattaaaaac tagtgataac
     3181 cttttagata ttatgcagac tgtaacaggg aaatctccat ttgtattccc tttgtctatt
     3241 caagatataa caccatatgg aaataatctg tatcacttaa atagtatttt acaaccagca
     3301 gtagctacag atgcaccaat agtaggagta gcaattacaa cagagatgcc tgttgctgga
     3361 tgtgctacag gtgcaactaa ttttagtgat ttagattcag ctgggagatt tgcaattgaa
     3421 gttgccaagc tatttggtag aaataagtgt gatttctatg ataaagaaga gtgggaaatg
     3481 cttattaaga gatatggcaa attaaataga tttcaaacat ttggtataca gtaattagaa
     3541 aaatattaag tttatataat ttgtgatata aatatctata ctatgtaaac aactagaaac
     3601 aaaagatata gtttaagact taaaaaataa aatatttatt tgattacata aataaggagt
     3661 gttctattta ggtaagcgcc aataagtagt ataacattaa agctaaccta attgttgata
     3721 tacaagattg aaatgaaaac agtgcgtatt ataagaaaag gttacgcact gtttttttcg
     3781 tcctgttata tagcagaaca ctagttttta agggtatgag tataaaactt ttttaaattt
     3841 catatctacg aattttgtag attgtccata agcgatatgc aataattagg aaaatatttt
     3901 gagtttattt ttcagcttta ataatcattg tgccgttgtt ttcatgaagt acatacacag
     3961 ctctaaagcc tgcatttttc attaagtgta tttgatgttc aatagtcaaa ggaatatcaa
     4021 tatggacaaa aacatcatca ggcacatgat tttctttaca tttaaattca tattgttcaa
     4081 gacaaattgt ttcttcctct aaacaacagg caatataatc gcactcaaca taatatcctc
     4141 caggtttaat tgtttgaaaa agctttttat atatcttctg ctttttctga tatttgaaat
     4201 gatgaagcgt ttcaaatgag agggcagcat catatttctc ttgctcaaaa ggatattcaa
     4261 aataatctgc caagattaaa tcaatatttt tatctgaata tttttcttta agcttactca
     4321 tcatttcttt tgataaatca atgcctgtaa tttgtacatt aggaaatttt cgataaatcg
     4381 atgcaagttc tagaccagta ccacaaccaa tatcaagtaa agaagatagt ggagtatcaa
     4441 aataatctgc tatatgtgca tattcttctg tccaattagc aagatgaaca ttatcataaa
     4501 tatcaattcg ctttgaaaag aaatccgaca tttcttcaac cggtttactt ctttcttgcg
     4561 ctaaccattt tttgaggtca agcatatatc ttttgatttc ttcaggtgtt cttattttac
     4621 tttccaacat ataaaacagt cctttcttta ttcggtcttt gttatatcca atgaaaatcg
     4681 ctcaaaagca ttttcaagta actgttgtac catatctaat ggaatgtcta tgccattgga
     4741 aataatttgt gtagctactc catgagaata aagccaaaag ttaataaata gcatttctga
     4801 tttttctata gataagcctg taactttttt agcattttct atagtaattc tgttccattt
     4861 tgcatgaata atatcttgta aggatgaacc cttcattgtc atattcataa ataaggtatt
     4921 gaaaatcatt ttttcatttt gagcaaattc aatgtatgca agtccttgac ttaacagacg
     4981 attttcatca tttattttac tttccataaa agaattatag taattgtcta gttctatttt
     5041 aatatcttct tttatctcat ccatatttgt gtaaatacga aaaaggggtt ttgtagagca
     5101 attaagggtt tttgctaagt tacgagcatt tacgctactt attccatttt ctcttaccaa
     5161 ctgaattgca gcgttaataa tatcttgttt tgtaataatc ggttttggtg gcatcttttc
     5221 atcctccttt cggtaactta agttgcgcaa ctggtgttgt tattataaca aaggcatata
     5281 actgtgtcaa tagacttcaa agaaaagcta cagacatcga gtgtaatatg atttatttca
     5341 ataaatacca taatatgtgt acttatatct taaaaaggag ataaacagta aaatgtagtt
     5401 tagttaaatt tgaaacttcg tcaaagcttc tttgtacgat aagacaacgt acaaatcatt
     5461 gtatgggtaa cagagaaaga actgcgttga ttgaggatga aatctcacat gatacgatgt
     5521 aattataaaa aaaggagcgt ttcaaagtga tttttttaat catcttgaga caactcctta
     5581 tattttttat ttaaattcta ttttttattg cctgtatagc tattttctcc atcataaggt
     5641 ggcatgacct tgaacagcgt ggttttgtaa agttgggcac cacaaaacaa aaaatcaaca
     5701 agcccttgtg tggtctgctg acgtgtttac tatactatac tttatccaaa accgcaagca
     5761 ctatgggagg ctataaggcg gcggccattc aggggcgatc ccctcgaaac cgggataggg
     5821 ttcccatgtg ggacataata aagagcgccc cggaggtatc aactagcatc tggggcgctc
     5881 tttattatgc cccgtgggaa ccatgcccac gaagcagggc gtacaacgga cagataggtc
     5941 ctaccctcct gacaaactgg aatctccgaa aacaatcata acaatcatgg caagattata
     6001 tacactcctg taaaatattt ttgtgtgtac catgaagtac tgcttgccac tattgagaac
     6061 ggataaaagg gaagtacgta ttataccaat aaggtgcatg aaacaaaata ctcatttgat
     6121 aaagttaaat aaacctgtcg gaaccccacc aatccggcaa aagctcactc aatttaacag
     6181 ttttttctgt ttcgacatta actaaaatct caatacaagc actatcctta tcaagttgca
     6241 ttaaatattc acgacaagca ccacatggtg agccaacact cccattgctc atcacacaaa
     6301 ctaatttagt aattttactt tcgccatttg ttatcatatt ggcgatagca tttctttctg
     6361 cacacattcc caatgtacag gctgtatcaa tacacacacc agtataaaca tttccgctgt
     6421 cggtaaggat tgctgcggca accccaccag catcgataaa aggtgaaatt gtgcgtggat
     6481 ttaattttcc cctagcctta ttataaagtt ccttccattt catcaggttg atcctcctgt
     6541 tgtatccata taaataatct tttatacgcc ttttccgcct aaggcacaca acacattact
     6601 cgcttttttc attctaatat tcgtgcgtga aaatgtcaaa cggtttactt cgtttcatta
     6661 cttcatactg ctgttcataa ccctgtttgt tcacttgggc accattagag caccaattta
     6721 cccgtttcag ggttgtagcg aacacagcag aaatgctcat ttcatgggct tttctggcta
     6781 gaaaaaaaat tttatcgctg ccagatattt actatttttt attaagttgc tggagttcca
     6841 ctaccatcag gaggagtagt agagccacca ccattatttc caccattatc aggaggagta
     6901 gtagagccac caccattgtt tccgccatta tcaggaggag tagcagagcc accaccatta
     6961 tttccgccat tatcaggagg agtagtagag ccaccaccat tgtttccgcc attatcagga
     7021 ggtgtagaac cgccaccatt atcacctgtg ttattattgt tattattatt ttcttctggt
     7081 tggttattgt ctggtttttt tgtatcttca gatgatggtt gagaacttac tttagtagga
     7141 gcagtacctt caataaatgc tgctttagag ccagaagatt gctttcctcc atcctctaaa
     7201 ttaatttttg taaaatatac atttttagga acttcaaatt cagtaaccgt taaattagca
     7261 tgtatttttt ccataatttt ttcccatagt tttgcagtac tagtacttcc atgtggtact
     7321 tctctacgtt ttatagtgtt accagaatca tcttttctac cagcatcatc tccaatataa
     7381 gttgcaccaa cataataagg agtgtaaccg acaaaccaag catcatatgc actatttgta
     7441 gtacctgttt tacctgctac aggcattccc tttggaagtg cagcaacacc accagtacct
     7501 tcagtaatta cactttctaa catatctgta acgacataag ctacttcagg ttctacaact
     7561 ttatgttcct caggagcatt tttaactaat aattgaccat caaaggtagt tatagtagta
     7621 aaaatagaag gttctacata aataccgtga tttgccaatg taccataagc agcagccatt
     7681 tgaagtggag taataccatt cgccatacca cctagtgtca atgatgggaa ttttctatca
     7741 cttgcttcag atgaacctga gtgattattc tttaaagttg ttataccaaa gttctttaag
     7801 taatccatca ttatatctac acattcatca taagagtctc caagcatttc agcagttttg
     7861 acagctatcg tgtttgaaga tttagcaagt gctttacgta aactcataga accatgacca
     7921 gcagtagtag ttcttggatt ccatttatta ttttcttcaa acttatatcc tcctcgagaa
     7981 tcacttagag cagttgcttg tgttattttt agagtatcaa tagctggcgt atatattgct
     8041 aaaggtttta tagaagaacc tggctgatga gggttagtag cacgatttaa agtttttcta
     8101 ccacttatat ttcttcctcc agctagtgct cttatttcac catttttata atctaagatt
     8161 accattgcag cttgcggttg gctaactcca tcaggaccga ccatatgtcc tgggaaattg
     8221 ctatttctat caaattcttc ttctaaagca tcttgcattt tagggtctat agttgtgttt
     8281 acaattagac caccattgta gaataagttt tgagcttcat catttgtatg tccttgttcc
     8341 attaaagcat ttataacttc actttcgata aggtcttcta cagatgaaga aactttatct
     8401 gatgattgag gcaattttat ttctatttta gctttgattg cttcatcata ttcactttgt
     8461 tttatatatt tcaattctaa cattttcttt aaaacaattt cttgtctttt tttagcttca
     8521 ggattactta cagcctttct aacaactaat gtacctgctt ttaattgtct ataagtatca
     8581 ttagatataa gctcccaaga ttttattttt tcaatcatat caaagtccac ttgagtaggg
     8641 tcatctaaat catcatcagt tgtgttaaca aaaaataata atttattctc taaatcctcc
     8701 ttagtttcat ttccctctaa cttagaagtt ttataggctg aaaatcttga tggattttta
     8761 gttgaaccag caagaagtgc tgactctgct agagtaaggt cagctgcaga cttatcaaaa
     8821 taaccacgtg caccagcctc agcacctgct agacctctac caacgaagaa gttatttaaa
     8881 taagtttcta gtattttatc tttacttact gttttactca tctcatgtgc atagtaaata
     8941 tcctttattt tacgaggtat agtttgctgt tcagttgtta acagcatttt ggatacctgc
     9001 ataggaatag tactaccacc ttgcttagta cctgtaaggg tctttaatac tgacctaaat
     9061 aaacctttaa tatcgacacc cttatgctca taaaaacgct catcttctat tgcaattaca
     9121 gcatgttgaa gataagggga aatcttatct aatggaacgg gtgtcttttt atttacactt
     9181 ggagctgtag ataatgtttt tccattagcg tatttaatag tagttgtttg gttcgtctgt
     9241 ttatctaaaa cagcttctgt aactggagtt acatctctta gtgacgcaaa tactagacca
     9301 gcagtacctg ctgtaccaac aactagtaaa actaaaaata ctatcccaaa tactcttagt
     9361 tttttaaatt tatcgctttt tttagatttt ttcttgtttt ttgttgaacg agtcttgtta
     9421 gctgaagttc tattaattgg tttatttgta ttaccagatg agctaacttt ttttctcctg
     9481 attttatttc catttttatc attatcgtta ttcataagat tttaacctcc ttacttaatg
     9541 caatattata acacaaatag gagtagtttt gtaattaata gaaaaaatga atttgctata
     9601 atattctgta cattgtatat gtataaacgt aatatattaa aaagtctata tttttatttt
     9661 atttattttt gacaaatatt ataaaaatta tgccaaagct acatataaat aaataggagg
     9721 ggataatttt gaatagaata gtagatagaa aaacaaaaaa tgaatttaga aaaatcatag
     9781 ctattttttt agtaattctt ttcttgtctg tatttatagg cagttttata tatatagata
     9841 aaactttaag accgacaata acagttttag cagagactaa agctcttgaa ctagcaaata
     9901 ggtcgataaa taaagctgtt gctgagatgg tagaaggaaa aataaattat gaagatttaa
     9961 tggatataca acttgataat aatggtaaaa taactatgat acaagcaaac actataatga
    10021 tgaatgaaat tgcatctgca atagctttag aaatacaaga tgaattaaaa aaggataaaa
    10081 cagcaagttc ttacatacct ataggaacag cattaggaag tcctatatta gctaaatacg
    10141 gtcctaaact tgaagtatct attgaaccta taggaacagt atcagtaaac tttaaaacgg
    10201 aatttgagtc ttcaggtata aatcaaacta gacatagaat atatttagaa gctcaaacac
    10261 aagttaaagt agtaatacca cttataactt caacaaaaca aattaaagct caaataccta
    10321 tttgtgaaac tataatagta ggtgatgttc cagaaagtta tgtaaatatt ccagaaaaaa
    10381 atctaggaaa tgttttacct aacactggaa agaataccaa caagtaatat aaagttgtat
    10441 atttttaaat aaattcaa
//
LOCUS       DAEOSP010000137        10434 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.139, whole genome shotgun sequence.
ACCESSION   DAEOSP010000137 DAEOSP010000000
VERSION     DAEOSP010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10434
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.139"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(61..249)
                     /locus_tag="KRH54_002636"
     CDS             complement(61..249)
                     /locus_tag="KRH54_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580280.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
     gene            583..1551
                     /locus_tag="KRH54_002637"
     CDS             583..1551
                     /locus_tag="KRH54_002637"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBG8580281.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            complement(1668..2798)
                     /locus_tag="KRH54_002638"
     CDS             complement(1668..2798)
                     /locus_tag="KRH54_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8580282.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFRINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(2951..3904)
                     /gene="msrA"
                     /locus_tag="KRH54_002639"
     CDS             complement(2951..3904)
                     /gene="msrA"
                     /locus_tag="KRH54_002639"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBG8580283.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            complement(4028..4933)
                     /locus_tag="KRH54_002640"
     CDS             complement(4028..4933)
                     /locus_tag="KRH54_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBG8580284.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            complement(5304..6896)
                     /gene="hcp"
                     /locus_tag="KRH54_002641"
                     /gene_synonym="priS"
     CDS             complement(5304..6896)
                     /gene="hcp"
                     /locus_tag="KRH54_002641"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBG8580285.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            complement(6956..7681)
                     /locus_tag="KRH54_002642"
     CDS             complement(6956..7681)
                     /locus_tag="KRH54_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG8580286.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            7822..8550
                     /locus_tag="KRH54_002643"
     CDS             7822..8550
                     /locus_tag="KRH54_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG8580287.1"
                     /translation="MKTNYRLHMQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEM
                     LECLGVVDKTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLF
                     GEVFACTRLHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKN
                     ILLHNKIELLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRE
                     LGKMRDEGLLSFNKNKFEIFYFQK"
     gene            complement(8714..9985)
                     /locus_tag="KRH54_002644"
                     /pseudo
     CDS             complement(8714..9985)
                     /locus_tag="KRH54_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416360.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
BASE COUNT         3484 a         1746 c         1193 g         4011 t
ORIGIN      
        1 ttacttattt tcttatataa taaaatgtat attaaaacat gttaaattgc aaaataagct
       61 tcaatctctt ttactaattt catccattat taatgatttt gaaatataca ttacttctat
      121 aatattctta aaacgtgtat gatgagatgt ctcctcagaa aatttcttct gtccttttcc
      181 acaattaata atacttgaag atactactct cagtgcttcc accaattctt cttttgtatg
      241 ataattcata accatttact cctttatttt ttaatttaga gaaacagtat atctcaattt
      301 aatatcatta taaactgaca tcattattat ttatacacat cttttagcac ttaaatattc
      361 taactatcag tgacattaca tattttaata attattaata tacatataat actttaatat
      421 tcactaatag agagtatatt aacattgctt ttttaaaaat tttagtataa aatcatatta
      481 aataaatttt ctgaaataat agaatattaa aatatccaaa aacagctttt aataacatct
      541 ataattttca atgtatcgtt attttagggg gagtgattta aaatggcaat aaagccaaga
      601 aaaatatcta ttgtaggttc agggcatgtt ggttctcatt gtggatttag tttaataact
      661 caaggagttt gtgatgaact ctttatgatt gatatagatg aatctaaatc aaaagctcaa
      721 gctttagatt tagctgatgc agtttcatat ctgccacata aagtacatat agaaaaagga
      781 actttttctg actgtaaaga ttctgacata gtagtgataa gtgttgcaga ctcttcagaa
      841 gggcctttac gtagacaaaa cacaactaga ttagatttac taagaccaac tattggaatg
      901 ataaaatcta tagtaaaacc catagttgat tctggctttg atggtatatt tgtagtcata
      961 tctaatcctg ttgatgtagt tacaaattat atttgggaaa aatctggctt tcctaaaaac
     1021 aaagtcattg gaactggaac agctttagat tcaacaagac ttagaaggat tttatctgaa
     1081 gaaactggaa tcgctcaaca atcaattcaa gcttattcta tgggtgaaca tggagattct
     1141 caaatggttc cttggtctca tgtttcaatt ggtggtaaac caatcttaga catgataaaa
     1201 gacaacccaa gtacttatag caatttagac ttaccatcaa tagttgaaaa aaataaaaaa
     1261 acaggaataa gtataataaa tggtaaagat tgtacagaat ttggtatagg tactgcttta
     1321 gtagaaattg taaaagctat actacacaat gagaaaaaag ttcttccagt ttctacatta
     1381 ctagaaggtc aatataacga aagaaatgta tttgcaggtg ttccatgtgt tataggaaaa
     1441 gatggtattg aagaaattat tgaaatcaat atgactgaat atgagcaaaa cgaatttaat
     1501 aaatcttgtt ctgttttaag ggaatatatt gcactttcaa aaacacttta gattcaatat
     1561 tctttcttac actaaaagca ggtaaatgac ttattgaaaa aacttttaac taagttaatg
     1621 tttaaatcat ttgtacctgc ttttaatttt aatactaaaa tataaaccta ttttttacca
     1681 tcaaatttta taactttact ttttttattt acctcttgag ataacaatcc ttttttcaat
     1741 cccatcaata attctaagtc ttctggataa agatttattc gaaattcctc taaacttgat
     1801 actatactat ttacactcat tattttattt tctatcaata attcaactgc tctttttaaa
     1861 tacgttgggc ttgaaccttt cattttgtct agtggttctt ctatcaacca gcctttttta
     1921 tccatttcat tcataagata attgtatttt ttgtaattta tcttttctag attatatgct
     1981 ctaaaaatca tagcagatat aggtacaatc cattttttct ttagttctac ataataatct
     2041 aaatcttctg gatgagtcaa atcttttaaa aaactctctt ttggcaataa aaatgcacag
     2101 gcatactcat cttctgaaaa ttgcttactt ggtattctta attcacttga tattatatat
     2161 ccaagttcac atgccaaagt ataatttcta attgatgctg actttttgtc attacccaga
     2221 gatattagat atttagattc tttactaatt ctctgttttt gagtaaagat agttgcccct
     2281 tttctatcta cattcatacc tgatattata atccctttag actctaacaa acttaccata
     2341 tttactattg gagtactatt taaatcccaa aactctctag tcttatatgc aagagattct
     2401 atatcattta tattaatatc tgacattact tttctatttg gcaagttttc ttttggaaac
     2461 ttaatataat tctccataaa tgaatatatt ttatgtatta taattatttt ttctctataa
     2521 gaaatctctt ctactctagg tagtttagat tgagggttaa agtggctatc ctctacaacg
     2581 atatttatat tttcattttt ataaaaatac tcttttggaa aatttaatat attagataat
     2641 tttaatgtat tttctatatt tggtacatac ttattttcct caaaagcttt taaatctttt
     2701 ttatttattt tagtttcttt agacaactgt tctatcgtct ttgctctaaa aattctagct
     2761 gattttaatc tctttccatt aaagtctttt aaattcattt gttttctctc tttcaaaatt
     2821 aaattcttaa tctattggta taaataagat tacaattaat aatttatcat actattgaca
     2881 aaaaaacaaa aggtgaaaat atttttttag attttcacct tttagataag tttataataa
     2941 atattcctaa ttactcaaaa agttttaaat attctccata tcctaaagct tccatatttt
     3001 ctcttggtat aaatttaaga gaagcagaat ttatacaata tcttaaacct ccaagttctt
     3061 ttggaccatc atcaaataca tgacctaaat gagaatttcc tttagcactt cttacttcaa
     3121 ctctgtacat accttgtgag aaatcacttc tctcaatgat agatttatct ataactggct
     3181 ttgaaaaact tggccaccca catccagaat caaatttatc ttttgaagaa aataacactt
     3241 ctccacttac aatatcaaca taaattccct ctttgttatt gtcataatat tcattttcaa
     3301 aaggtacttc tgtcatatca ttttgagtaa cttcaaattg tataggagtt aaagtctttt
     3361 ttaactcttc cctatttata ttgtttccat cccaatgact ttttacaaaa ttatatcttc
     3421 cggacccttt ataatacata ttgtagttta atggtctttt cttataataa tcctgatgat
     3481 tttcttctgc ttcgtagaat ttagttgctg gaattatatc agtcactatt ttactatcaa
     3541 atattttact attttccaga tgttctcttg actctaaagc tatatttttt tgttcttcat
     3601 catgataaaa tatagcagtc ttatattgag ttcccctatc accaaattgt ccaccatcat
     3661 ctgtaggatt aatctgtttc cagaagatat ttagaagttc atcataactt attatatcat
     3721 catcatatgt tatttgaatt gcttctaaat gaccagtagt tcctgagcat acctcttgat
     3781 atgtagggtt ttcaacatga ccacctgtat atccagaaac tactttaata actccatcat
     3841 acttatcaaa aggttttacc atacaccaaa aacatccacc tgcaaaagta gcaagtttta
     3901 tcattttcat cactccttaa ataaattata ctttatttat tccctaagaa gtaacttaat
     3961 aatctgcatt ttattatttt tacctactca atgaaatcat gtgtaaaaat acaattgcaa
     4021 attgaatcta atttctataa aagctctttt tctttatcca tccaaaagca tcttttatat
     4081 cacaattttc tcctaaagac attagatatt ctaatgcatc acaatcatcc atagctacat
     4141 ctttttccat cttaggattt aatctatagt aattatctcc aagaagtttt tgagaaaata
     4201 actgactcat ttgagaattt ccttctaagg taatagatat aattggtaaa tcgggttctt
     4261 tattaattat ccagtcaata gctccccatt cagtagtatc tgcctttata ctgttatata
     4321 catatccagt ccctattgaa aaaagtcttg tattcttaag tttaaatcca ctgtcaatgg
     4381 catgaattat acacgctaaa ctcgggtcag ttgctattat tcctccatca acatgacgat
     4441 tatatgttgg aaaaaatacc ggagcagcac tacttgccat tgctacatct acaactttgc
     4501 tagtctttgt aaaagaattt ggcatattgt tataaaacac tggtttccat gcattttcct
     4561 catttcctat ataaaaactt ggtatcatta ctaatttatt taaatctttt aattctagtt
     4621 cctctggaaa tatacttaat aaaacttctt ttaagttttt gttttcatac tttggtctga
     4681 gtatttctgc ataactttta ttaaaaatat actttgaatt ttcaattgag taaagttctt
     4741 ttatctcttt agggcttaca ccatacgcaa gccctagtgc aattagactc cctgtagatg
     4801 tacctccaaa catatttata ttgtttaata gatttggata ctcttttact atcctctcaa
     4861 gtatggatat actcaatgcc ccttttaatc caccaccatc aaatgccaat atattaaaat
     4921 tagaattttt catatacata tacccccaca taaaacacct tatattttaa attttaatca
     4981 aaataatttc actatagtat atatatttta tgcaagttac atattacaat gttcctcttt
     5041 taatatttaa gttacattca aacctttaat acttaaaata aaatatataa gatattatat
     5101 atctaagtat tataaattta atacaaaaat tcttaaaaga atatactgaa agcaggttta
     5161 tttttcaatt attacaaaaa ataaataaag ggtcatctca aaattgattt tttaatcaaa
     5221 ttttaagttt aaccccttat cttaattgtg gcatacttaa aaatatttta actataaata
     5281 tctttaagta tagatatatt tatttatcct aaaatcttct tcatatcttc ttcaggagta
     5341 gatataggtg tcaatgcaaa tttatcaacc aatatatcta atacattctt agataagaat
     5401 gctggtaaag aaggccctat atagatattt ttgataccta aatgaagtag tgtaagaagt
     5461 atacaaacag ctttctgctc ataccatgac agtaccatgc taagaggcaa atcatttact
     5521 ccacattcaa aagcatttgc caatgaaact gccactttaa ttgcactata agcatcatta
     5581 cattgtccca tatccattaa acgaggtaag ccacctatat ccccaaggtc taaatcatta
     5641 aatctatatt ttccacatgc aagtgtcaat ataacagtgt ctttaggagc ttgttttaca
     5701 aattcagtgt aataatttct tccaggtttt gcaccatcac aacctccaac caataaaaag
     5761 tgtcttatag ctccactctt tacagcatct actactttat cttgtacaga tgaaactgta
     5821 cctcttgcaa aaccagtagt taatgtatct cctccattaa taccagtcat atgaacatct
     5881 tctctatatc ctccaagttc aagagctttt tctattacac tagaaaaatc tttatcttca
     5941 tcaatatgaa ccatacctgg ataagcaact acttctgtag taaatactct atctctataa
     6001 ctatctttaa caggcattaa acagtttgtt gtaaataata ttggagcagg aatattatca
     6061 aattcttttt gctggttttg ccatgctgta ccaaaattgc cttttagatg tttatattct
     6121 tttagttttg gatatgcatg tgctggtaat aactcaccat gagtatatat atttataccc
     6181 ttatctttcg tttgttctaa taatttgtgt aaatcataaa ggtcatgtcc tgtaactaca
     6241 ataaatggcc ctttctcgat tttcatagat acctgagtcg gttctggtgt tccataagtt
     6301 tcagtatttg ctctatcaag caattccata cattttaagt ttatttctcc agttttcata
     6361 actagaccta aaagttcgtc aatagacaag tctcctccaa ctgctattaa tccttcatag
     6421 aaaaattcat ttacgtcttt gtctgtataa ccaagtacca ttgcatgata tgcataggca
     6481 gccatacctc ttaatccaaa taagattaat gattttaatg aacgtatgtc ctcattatca
     6541 ttccataaat ccttcatatc atagttatca tttctattac aagttgttga acaatctgaa
     6601 catctaggta caagtttact tttttctacc tcagtttttt caatttgtct ttttatagct
     6661 tcatcatcaa aacttacatt cgttatagta gtgaatagcc cctctatcat aattttatct
     6721 gtttcacttg ttggtctatt cttatttcca atagttgctc ttgaaagacc aatcaacact
     6781 cctgtaagtt cgtcttgtaa gttagctgta cccccctttt taccacagac accatttata
     6841 gtacaacctt tacctcctgc tgtttgttca cattgaaaac aaaacatttt ttccattttt
     6901 tattcctccc tttttattta ttaaaattta ttatattttt ctataattca cttaattaaa
     6961 tttctccatc tacaattttt ccatctacag atatagtaac tatttgccat ggtatcattt
     7021 ttccactatt ttgtagagct gtcttaacag catttactat accaccacag caaggaactt
     7081 ccatacgtgt tactactata tttttaatat cattttcttt taaaatctct gtaagttttt
     7141 cagcatagtc aacttcatca agtttagggc atccgattaa tgtaacctta tttttcataa
     7201 atttattatg aaaatttcca tatgcatacg ctgtacagtc tgctgctatt aaaagacttg
     7261 cattctttaa atatgatgca ttagttggta ctaattttat ttgtactggc cattgattaa
     7321 gttgagaacc ttttatctct actatatcat tgcttataga cgtactattt gaaacctctc
     7381 tatttattga tttagactga gaacctggac atccacatgc taaagttttc ttttgtgctt
     7441 tctttgcttc catatttgct tttactgcag cttcatcata ttctgctgct tctctatact
     7501 caaatgaaat cgcatttgtt ggacaagttg gcagacaatc tcccagccca tcacaatagt
     7561 catctcttaa aagttttgct tttccattta ccattcctat agcttcttca tgacaagctt
     7621 caacacatag tccacaacca ttacactttt cctcatcaat tttaattatt tttctcatca
     7681 tttatatcac ctcataaaat ttttttaatt taaagtattg atttcttata tatttatttt
     7741 taatacctat atttctttat ttcaaatttc ttgtagtagg atttttatat ccccttgctt
     7801 ttataaactt agtatagtac aatgaaaaca aattatcggt tgcatatgca acaaggaagt
     7861 gattatttga aaaaattttt agaaaagcat atagacctac ttaaaaaaat tcctctattt
     7921 gaaggaatta aaagtgatga acttgaagaa atgttggaat gtttaggtgt tgtagataag
     7981 acttacccaa aagatagtat aatattctct gttggaagtg aagttacttc tattggaatt
     8041 atgcttaaag gaagtgctca tataataaag gaagatatcg aagggaatag gaatattgta
     8101 gctgaactct tgcctggaga tttatttgga gaggtattcg catgtactag actacataaa
     8161 agtaatgtta cagtaaccac aacatcttct tgcgaagttt tatttataaa gtttaaaagt
     8221 gtaactggta tttgttcatc tgcatgtgta tttcataata ggcttgttga aaatatgcta
     8281 caattaatag ctgaaaaaaa cattttactt cacaataaaa tagaattact ttctcgtaaa
     8341 acaactcgcg agaaattgct tatgtatttt tcaaaacaaa tagaacaaac aggtagccac
     8401 cagtttacaa ttcccttttc aagaaatgaa atggctgatt ttttatgtgt tgacagaagt
     8461 tctatgtcaa gagagcttgg caagatgcga gatgaaggct tgttatcttt taacaaaaat
     8521 aaatttgaaa tcttttattt tcaaaaataa aatagatatt taaacatatt tatttcaata
     8581 tatactatat tagttgattc aaattataaa tatcatattt gaatcttaaa tttatatttt
     8641 tacttaaaaa gttaataggt atctaattta ttaaaattaa gatacctatt aactttatta
     8701 attaagtgta caattagctt attttctttg tagtaattgc tgttttatct ataagccaat
     8761 cttttccttc tactaatctt gaaactaaca ttgatgatac tgtatttcca gttgtattta
     8821 atatagtatt aggaatatca gttatagtag ttataactgt taacagtgga attgcctcca
     8881 ctggaaaacc gaagatgcta accacgagca tctcaccaat aagtcagcca cctgctacag
     8941 gagccataaa tactccaaca acaaatacag ttataaataa ttgacctatc gttgctggat
     9001 ttgccattcc actacctaaa atacctgata aaaatgccac ttttattatt actgctaaaa
     9061 ctgccccatc tttatgtata tttattccaa gaggtataac tgtttctgat atatcatcag
     9121 gaactcctat atttttacta gcttctaaat ttgatggtat acatgctgaa cttgaagaag
     9181 tagctagtgc cgttgctatt ggttttatag catttctcca gaattgtcta aacccttgtt
     9241 ttcctgcaga taaaaaagca tacagactgt aaaataataa gaaatacatg gctgctgtta
     9301 gtgaataaat tacaaacacc cttacatatc cttgaattat tttacttcca agttccccaa
     9361 ttattgtagc tgtataacag cctagcccta taggagcata aagcatgaat atacttacta
     9421 cttttaatat tgcttccgtt ccttctgata aaagctttct tattgtattt cccctatctc
     9481 ccatcatagc tattgctgtt ccaattaaaa ctgcaaatat aactaattgt agtaaattac
     9541 tactggaaaa taaatctcca aaatttttaa ctgtaatact tgatactact tggtctaata
     9601 acgttccttt tgattctaaa gtttctgcat cttgagccac cattaaattt tttacacttg
     9661 atacatctaa tcctttagac ggattaaata ataatgcaga tgtaatacct aaaaatgcaa
     9721 ttacaagtgt tgttcccata aatactatag ctgtaccctt cattattttt ccaaatcgtt
     9781 ccatattttt tatactagca acacctgaag aaacactaag aaaaactaat ggtactacta
     9841 tcattaatag agcatttatg aataaatctc caaatggttt tagtattgaa gcttttggac
     9901 caagtactaa tcctactatc gcccctacta tagtacatgc taaaagtata attgatgact
     9961 tataattttt taaaaagtcc ctcatttata ttttcctttc taatgcttta tattataaat
    10021 taccccaaat gctaaaaaaa ttaaagcaaa ttatctaatg cgcattaata atttttgtaa
    10081 ttttccccaa cagctacttt agcaaatact ggaatatatg tcaatttcta tagttttttt
    10141 gtatttttaa cgttttatta atagttcata tctatatata aatattaata aaattctgct
    10201 tttaaaactt tttttctaga ttaattttaa taaaacaaac aatataaaaa aatatatata
    10261 atattactta attattttca aatttaatac tttgtacttt aatataaaat tttagtatat
    10321 tcattgtaat tcctatacta tataactcca taaagttgat tttgtaaaca catatgatac
    10381 tttgtcaaat attatttttt tatgtatttg atattttgat atacttttaa aaag
//
LOCUS       DAEOSP010000138        10325 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.140, whole genome shotgun sequence.
ACCESSION   DAEOSP010000138 DAEOSP010000000
VERSION     DAEOSP010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10325
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.140"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(142..876)
                     /locus_tag="KRH54_002645"
     CDS             complement(142..876)
                     /locus_tag="KRH54_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8580288.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMYSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
     gene            complement(1882..2520)
                     /locus_tag="KRH54_002646"
     CDS             complement(1882..2520)
                     /locus_tag="KRH54_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBG8580289.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            complement(2523..3380)
                     /locus_tag="KRH54_002647"
     CDS             complement(2523..3380)
                     /locus_tag="KRH54_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580290.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTEYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            complement(3382..3756)
                     /locus_tag="KRH54_002648"
     CDS             complement(3382..3756)
                     /locus_tag="KRH54_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8580291.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYQED"
     gene            complement(4905..6095)
                     /locus_tag="KRH54_002649"
     CDS             complement(4905..6095)
                     /locus_tag="KRH54_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBG8580292.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(6111..8066)
                     /locus_tag="KRH54_002650"
     CDS             complement(6111..8066)
                     /locus_tag="KRH54_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG8580293.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKSGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGVDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     NEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAASQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            complement(8083..10149)
                     /locus_tag="KRH54_002651"
     CDS             complement(8083..10149)
                     /locus_tag="KRH54_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBG8580294.1"
                     /translation="MKEVVGSLKKITKERTENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYIYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
BASE COUNT         3502 a         1823 c         1210 g         3790 t
ORIGIN      
        1 aatacttgta atactacgta tcatccttaa cacttaagta taattttaaa gcaaaattct
       61 tattacttat atttaatttt gattctacat ctttagatat aattttaagt aatttaagaa
      121 attattgtaa atcatatagt actactcaat aattaggttt ttcaatgttc caattatgtt
      181 tctttgatat aatccaccat gactaaaaac tatcgtttct atatcataat tacttatatt
      241 tttaatagca tcttttaact cttgtttgtt gctgtacatc acatcaacag gcttaagttc
      301 tccattttct acctgtaaca aatctccagc aataagtact ttagactctt ttaaataaag
      361 acatatgtga cctctagtgt gacctcctgt atgtataact tctatttcct cacctagatt
      421 caatttttca tggttatcta atgacacatc tactttagta tatgatgatt taaatccaag
      481 tcccataaca tttagcatac taagcatctt atcatcaatt ttatctacca tttgttccaa
      541 catataaagt ttaaaaggag tttcttctcc tgttatatag ctgatttcac ttttgtatgc
      601 atatactttg atattattct tagatttttc tcttagataa tttatgtttc ctatatggtc
      661 tatatcatga tgagtaatta ttatattttt taacctatca aagctcgtgt tacttttatt
      721 aagagcatca attattaaat caccttgacc cggaagacct gtgtcaataa gtgttgcatc
      781 atcttctgtg tagattacag gaatatacat aactttatct tcaccgaata catttgatga
      841 aatttttaaa acatctaatc cctttgctaa attcataatt tttctcctta taattactta
      901 attagaggtc aatttaatta aagaaattga gaaaatgtag attttcctct agttatattt
      961 tattaattaa aaattattaa tacctagtat atcatttaag ttgaaggatt actcgacttg
     1021 atttttttgt tattttatgg tatagtaatt aatatagaag taagtaattg caagctttgc
     1081 aattactttt atttttttct aataactatt ttatttttga taattcgtat aaaattaaaa
     1141 gtaaagtaat atagaaaata tgaatataga aaaactttaa taagaaaata aattatgaat
     1201 aaataaaaga aacgcaatac tgtatataga tgattattta gaaaaataaa atctctttta
     1261 ataaaaataa gcttcaaaat aaacttttag ttcataaagc ttaggagtaa ctaaattgag
     1321 tgcaagtgta tctactgttc aaaataaata gttcataaag cactcttttt tatattaaat
     1381 caattatatt ttcgtcattt cagcaatgaa aaagaagctt atctctattt tgagacagct
     1441 tctttttatt aacgtcctac tttttattag ttttctaaaa ttaaaaagct ttatttatag
     1501 gattccttag atttatattt tgacgatata attttttaaa aacaccttaa aagatggtat
     1561 acaatacaca caatcatact cataattgaa aaataatcaa tggaaaacaa aattactgga
     1621 tattccctat taaaaaatac aaattgattc acaagaaaca tataatctat aatattatga
     1681 tgttactctc ccacaccaag taactttatt ttcaagtaac cttttcatac catctaactt
     1741 cactcaataa aaatggcaat ataaatatat actctatttt ttacttttaa atatctgaca
     1801 agctttatat actattggaa gtaagataca aagtaaaaca gcccaatctg gtaaatgtgg
     1861 catataaaag tcccccttac cttatacaag ctccttatta ctaaaaattt taatagaaat
     1921 aaacattgaa gctaacagta taattaaact taaaaggaac aatacaatat tcactatcat
     1981 ggatggatac tgatatataa atggaatttc aaaattgctg tgagctatag ttaatggtaa
     2041 tacaaatgat gaagccatga ttatcaccac gaaaaagaat tttgttgttt catatcctaa
     2101 tttatattga ataggcatat aaactccata aataatagaa cttattaata aaacaatcaa
     2161 aatatatgtt aagtcaaaag ttcctatttg tggaataagt aaattttcaa tccagtaaac
     2221 taatgtacaa tagataaata ccattgcaaa taaaacatac ttagattgaa ctaagccacg
     2281 tctaggataa ggagttgaac aaagtaatac agatgctttt gaatattgac tctcctttaa
     2341 taatagatac tggcacagca taaattctga aaatataatg gatagtacaa atcccaatat
     2401 ttgtgcaaat ggaggtaaac gccacaatat aaataatggg attcctattg caataaataa
     2461 tatcaacaat acatattttt ttacaatcag aatatctttt ttaactaaat gaatcaacat
     2521 aattactctc tcctttcaat atatgcaagc ataatatctt caattgtagg acgctctatt
     2581 aaaatatctg gcatattttt ttgcacttca ttaatttttg aggtaatccc tataaaacca
     2641 tagccggtaa tttctaaatt taaaaataat tttttggttt catcatttag aaattgaata
     2701 tctcctttta cttttctatg ttcgtccaat agagtatctt tttcacgctc taatataata
     2761 cgtccattgt caatcataac aatcatatct gctattttgt ctaaatcaga tgttatatga
     2821 gtggaaaaga aaacaccacg accacatttc ttcatatatt ctgttagtat gtcaagtatc
     2881 tgactacgga ttagtgggtc taagccactt gtaggctcat ccataattaa aaactcagct
     2941 ttatgcgata atgctaatgc taaagcaaat ttcattttca ttccttttga aagtgtatca
     3001 atcttttgtt ttgggttaag attaaaacgc ttcatatttt gttgataatc ttcttcacac
     3061 caattatcat atgaaggtgc aataatactt ttcatttctg ccattgacaa ctcctcatag
     3121 aaatatgaat catcaaatac aactccaatt cgatttttta attccttctc atttccatcg
     3181 aaatctttac caaagatttt tatttttcca ctatcacgct ttacgacatc caatatcgaa
     3241 cggattgttg ttgtttttcc tgctccatta acacctataa atccagttat acaatcttct
     3301 ggtaatgcaa agcttatatt agataatgaa aaatcggtat aagtcttatt taagttataa
     3361 acttctaaaa tattttccat attaatcctc ctgatataga atatccatca tttcttgtaa
     3421 ctctatctgt gtaattccta gtgcttttgc atccttaatc atatctgcca ttttttgttc
     3481 aactatacgt ctctttgagt ctcttagtat ttctaaattt ccagttgaaa taaatgttcc
     3541 tattccaact tggcgtgtaa gaaagccttc attttcaagt tcttcataca ctcgtctaat
     3601 agtcaaaata ctaactttta aatcattagc aaaatttctt atggatggca ttgcatcccc
     3661 ttctttcagt tcttcagtaa atattgcttc tttaatttgc tctttaattt gttggtataa
     3721 tggcttatct gatgtgttag aaatgagcac tttcattttt tatcaccccc tttttatcat
     3781 cttcagtgtg atgttgttat atacacacta tacacttaga gcactttttt gtcaatataa
     3841 aagatgtaaa atattactat gacaaaataa atatgaatga aatattagag attgttaata
     3901 gccataaaca ctttttaaaa ccacataaga tagaatgtat taaatattta ttaaaagtaa
     3961 gaattgaata tctaaaagaa aaagtaatcc tgtatgaatg atttgtttga tgataaatta
     4021 agttataaac tatttataaa aaacataaag gaataaatcc atttctaatt acaaacatac
     4081 taaaaaattt agatagtaat aaatagatag taataaatgg gatgaattta taagaatttg
     4141 gatagaggaa agagttattc aagagtgtag actagaggta aactctatat aaaaaaaatt
     4201 taaattggga tattattatg tatggaatat ttgcagaaat aaccttgcta taagtataga
     4261 agattttttt gattacaaaa gataatactc taaaatgtga ggatttcaac attcaatact
     4321 gtgctttaaa taatatcact ccaaaatacc atccaccttt tactataatt gaatacccta
     4381 attattttaa aaaggaaaac attagaaacg gaaaaccttg tatatataaa ataaaaaata
     4441 ctaacttcta aaacacggat actctttgat aaataaaaca aaaaataatt ttaataatta
     4501 aaattggcta tctcaaacta gagataagtc tctttctcac ttttaaaacg ttgaaaatat
     4561 attcgatttc gtacaaaaaa gagtgcctta gaactaaaaa gttcactttg tggcactccc
     4621 ttttttctcc tctttttaat tttttataaa aattcttcaa aaatattatt aaatacccta
     4681 tattatacat aaatcaaatt aaagttgcat tttagcaaat aaaaacaaat tataactatt
     4741 agcatagcaa ataaaaaatc tgctctctta taaaatcaaa attataatta taaaaagaaa
     4801 atattcttat aacaaattaa ctaatataaa attattttat aaattaatac tactttttag
     4861 cttctcctat aaattaacac cactttttta actatgaaac ctatctacaa cattggtacc
     4921 ataagaccag ctaccacaat agaaatcata caagccatac caccaactac cagcttaggc
     4981 ataatcttag acatcaaata tgctctttct tcttccgttt ctccaacagc attacacact
     5041 tcatttgaaa ttaaatatgt agttgggaat cccaacatct gacaaaaccc tactccaaaa
     5101 gcaagaggtt tagaaccgat aattttccaa cctggtaaca ccttcatcat aaggaatata
     5161 ccaatgatag aagctgcaaa catacccaca attggtacaa aaagactaat taaatctgtc
     5221 aaagaaactt ttgctaaaga aggaataaca gctgcaaaca caaccatgtt gataaatccc
     5281 ttgatttttc ctttttccaa aatgtctttt ggtacaatac caatacgtgt tacaataaca
     5341 ccaacaacaa gacatacaat agaataattt acatgaataa tctctgaaag ttcataccct
     5401 agataagaga atagtgctat aaaaaatata caaacatttg tagaataata tttatcgtat
     5461 ttttcacaga aagcttgttt tacttttgtg cctgtattct ctgcttcttt cttatcagta
     5521 tcaacatttg ctaaatcaat gccttttgat tttgcttcat gatattctac taaaagacgg
     5581 tttgcctctt gtaacgctgc tcttgaagct attggtgtac caataaactt ctgaattgca
     5641 aatacaactg ttgctgtagc tgctgcaagt gtcagtcctt tttctagtgc tgcttgtgtc
     5701 ataatagtag tagcaggtaa agctccattt ataactggta tgcttgtcaa tactgttgat
     5761 tttccaaaca aaggaataca taatgagaca cagactataa ctgctacaat tccaagccat
     5821 gctgtcaata ctgttctcca ttcgtctata aattgtttaa cattcaacat tgttcccata
     5881 ctaaacataa tcattggaac actccaacta gctgctgcgg tcatacctgc tttttcaata
     5941 atatcagctg gtattgcttt tgtgacaaat agaatcaaaa ataaaagtag tgtaacaaat
     6001 acagctgata cttttgcttt tgttttgctt gcaattaaat ccccaatagc aaataaaact
     6061 aatacgacac taaaccataa taacatatca ctcatagaat aaccccctta ttataaatat
     6121 ctttcaaacc aatctacaat agcatttgct cttgcaattc tattagatgg tcttccattt
     6181 ctagacaatc catgtgtatc tccgtgaaac aaatacattt ctgtatctac accaagctgt
     6241 tttaatgctg taaacatttg gaaagtatcc cccataaaac atctataatc cctatcgcta
     6301 tgtaagaaca atgttggtgt cttaacattt ttcacatatt taataggtga atgattccaa
     6361 agaacctcct catgattcca aggcgtctta tctccacaag tctgcttcat aattctatct
     6421 ccaaagtcaa taattaaaga agctgtaatc aagttactaa tacctctttg agaagctgct
     6481 gctttgaaac ggtctgtatg accaattatc cagtttgtca tataaccacc gtaacttccc
     6541 cctgcaaccc ctaagcggtc tgcatcaatc ccaggatatt tttcaataca agtatctaca
     6601 aattccataa gatgctcata gtcaacatac ccccatcttt ctaccatttc taagaacttt
     6661 tcaccataac cgtcaccacc atgagggtta caataaatca catacattcc ctttgatgtt
     6721 acacactgct tcaagtgtgt aaacatatag ccatatgccc atttaggccc accatgaatg
     6781 aacaatacag caggatattt ttcgccttct ttagcatcta taggtggtat aacaaaacca
     6841 tcaacttctt ctttattttt tgctacaaac gtcaattttt caggtaatga aaccttatga
     6901 gtatctaaat atggtttaga aaaagcagtc atttttttca ttgtacctgt agtcatatca
     6961 atgctatata tttctgctaa ctcataatct ttcaacatta ctgtgtacat ggtgttgtcg
     7021 taaatatcaa aagatgtaaa agcatctact tttgtgatga tacgtatatt acctgtcttt
     7081 gtatcaaact cattaaattc tgtcatagtt ctgcaaattc ttgcaccaat aaatttatct
     7141 cctataactt tattactacg tccaccacca tatactgcat ctacatccag caatgtagca
     7201 tacataccat ctggactagc aatcatattt atttcattag tactcaaatc aagtataaaa
     7261 atatcatgat tctgtgtgat agaacggtct aatgttgaag cttgcaacat tactttatct
     7321 ttgccaatat attttgccaa acttatttgg tactttcctt gtggtacaag ttctttggtt
     7381 tcaccactgt ctatatgata ctcccaaagt ccagaaaaag gagttgcaca atcttcataa
     7441 atctgaccag tatataaaat tctgttttta tcagaagaaa cgtctacacc tgctacttca
     7501 caaaattttg gtgtaatggc ttttgttata ttttcacctt cttgatataa ttctaatgtt
     7561 acacgtcttc tttgagaaaa attttctcca tctcttataa aaggtttatc tgtaaatgtc
     7621 caataatcta caccctctac tgccctatct ggttctgatt ttttagaatc cgttggattt
     7681 tttgttgtca gtaaccacaa tttatttcca agtgatacag gtacgctaca agcttttgga
     7741 atagaaaatg ctttttttgc ttctcctcct tctatagaaa tagtgtaaaa atctgttgta
     7801 cctgattttg gcttatttct tttagatgta aaaaggagtt ctgtattaga aatccaacta
     7861 aatgatgtta catcttttgt ttgagtcata tgtatttgtt tttctgtttt gatgtccatc
     7921 acatataagt catgttcata gcagtctttt tcctcgttga tatttgatat aatataagca
     7981 atatgctttc catcaggtga acaagaaata tcatgaggga atatatattc aaaaatatct
     8041 gaactttcaa tctttatttt ttccattttt atattctcct ttctactgtc ctatgttatc
     8101 tttttttata tataccgaaa accaatctag catttcaact aatctttgta gcttttgttt
     8161 tggtctacca gaacgagaca gttcatggtt ttctccttca aatagacaaa gttttgtagg
     8221 acacccatga attttcaatg ctgaaaacat ctggaatgct tcttgcatat agcatctata
     8281 gtctttttca ccatgtaaaa ataaagtcgg tgtaacacat ttatcagcat aagcaagagg
     8341 tgactgtttc cattgaagct cgtggttttc catatgagaa gcacctgttt gtcctttgca
     8401 gaaataataa ccaatgtctg ttgtaccctc catgccactc cagtttgcaa tagaacgctg
     8461 agcacaagca acagcaaaac ggtttgtatg accaattatc caattcgtca taaatccacc
     8521 ataacttcca ccttcaacag ccaattttgt tttatcaatg tctgtatatt tttcaagtac
     8581 agtatctgta aattccataa aatctatata atcaatatct ccaaactgtt tcctaatatc
     8641 tgcaaaagca ttgccacgac cttcagaccc ccttgggtta cagaagaata caaagtaacc
     8701 tgcacttgta aatacctgta tctcatgcat aaaatatggt ccatatgtca atcttggtcc
     8761 tccatgaatc gccaatacag ctggatattt tttccccacc tcataatcta ctggtttata
     8821 tacgtatcct tgtatttccc atttagaact tccttttgac tctatgtatt ctggcacaga
     8881 aattttatat tcctctaaaa tatggtcatt taaatgtgtt ttcttacaca atgttccatt
     8941 ctctactgta taaacttctg gtaatccatt tcctaaacaa cccacacata caatattgcc
     9001 atcttttaca tctataaaat caactgaacc tgtctgtgtt actttttcct gttttcctgt
     9061 tggaatatgt attctttcaa taatgcagtc catatctact gtagaagtgt aataaatata
     9121 ttctccatct acctttgttg tctgaccagc acccatttta acatcagatg ttactgtatt
     9181 tccaatacaa tgatgttgat gccctgaaag aagtttcatt tctttcgtat caatatcaat
     9241 agtataaaaa tctccatttt ctccatttcc ataagataaa gcatcagtta ggtttagtat
     9301 cacttgattt tgatataatt caaaaccttt tataatatac ttatcttttt ctaatataca
     9361 gacatttgtg tttgtgtttg tatcaaatac atagacccca tttttcaaac cttttacatc
     9421 tttaaactct tgccctatat ataagatttt tccatcactt attttcatac ctacaacatt
     9481 aaacataggt gctgtaattg ccttcaactc atttatatta ctgtcgtata tatataggcg
     9541 ttttcttttt ctgctaatat atccctcccc attgacagca taaggaattt ctttgaaatg
     9601 ttcataatgt cttttgttat aagcatttat ttttttctgt tgttcttctt ctggcaatcc
     9661 ctctacaata ggtcggtcat tgtcataaga atcaataact gcatacaaac cttttcctat
     9721 aggattcaat ttaccacctt tgatagggag tctaaaaagt tcttctgctt ctccaccatt
     9781 taaagataaa gcatagtaac tcatgcattg cttaccgttt ttgacagctt cttttacttt
     9841 ttcatttctc aaagcaggga acaagatagt attttcatct tcccaggcat atgctccaac
     9901 agagtttata gacgttattt gttttaattc gccagtttta ttgtttaata tataaacttg
     9961 gcttgtgtaa ccattctctt tgatatcagc tttttttaca ataaatgctg tatatcctcc
    10021 atctggtgat atttggcaat cacttataaa ttgatattcc aaaaatgttt cactcgtaaa
    10081 tagctttttc atagtatttt ctgtcctttc tttagtaatt tttttcaaac tccctaccac
    10141 ctctttcatt ttttatttta aatttaaaat aaaaaatttt aaatttaaaa taaaaaattt
    10201 tataaaggtt atttttttag gaagccttct ggcttccttg taatatttgt aatttaaagt
    10261 atatttaatc catgcttaat tcatacttaa tctatactta atctatactt aatctatact
    10321 taatc
//
LOCUS       DAEOSP010000139        10305 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.141, whole genome shotgun sequence.
ACCESSION   DAEOSP010000139 DAEOSP010000000
VERSION     DAEOSP010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10305
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.141"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            165..944
                     /locus_tag="KRH54_002652"
     CDS             165..944
                     /locus_tag="KRH54_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG8580295.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
     gene            complement(1254..2075)
                     /locus_tag="KRH54_002653"
     CDS             complement(1254..2075)
                     /locus_tag="KRH54_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HBG8580296.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADILIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIVTHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(2809..3627)
                     /locus_tag="KRH54_002654"
     CDS             complement(2809..3627)
                     /locus_tag="KRH54_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8580297.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKTVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            3795..4376
                     /locus_tag="KRH54_002655"
     CDS             3795..4376
                     /locus_tag="KRH54_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8580298.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            4373..4567
                     /locus_tag="KRH54_002656"
     CDS             4373..4567
                     /locus_tag="KRH54_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580299.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(4731..6473)
                     /locus_tag="KRH54_002657"
     CDS             complement(4731..6473)
                     /locus_tag="KRH54_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBG8580300.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGNDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQNGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            6902..8278
                     /locus_tag="KRH54_002658"
     CDS             6902..8278
                     /locus_tag="KRH54_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBG8580301.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            8565..9236
                     /locus_tag="KRH54_002659"
     CDS             8565..9236
                     /locus_tag="KRH54_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBG8580302.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            9425..10222
                     /locus_tag="KRH54_002660"
     CDS             9425..10222
                     /locus_tag="KRH54_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBG8580303.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         3805 a         1247 c         1575 g         3678 t
ORIGIN      
        1 ctataatatt aacactattt tttatgctat ataacaatta ttgaaaatga tataaatata
       61 atatctcaaa taatatgaat ataacatctg aatagagggt gttttatttg taaaaaataa
      121 gtttacagtt tgagcgtata aacttatggt aaaggagaat aattatgagt tataagaaga
      181 ttgcaattat aacgggtgct aatagtggtt ttggtaaaga gtttctaaag ttattaataa
      241 atgaagaaga aataacagaa atatgggcag ttgcaagaaa caaggcgaga ttaaatcaat
      301 tagtagatga atttggaagt aaggtaaaaa ttttttcaaa agatttatca aaaatagaag
      361 aagtaaaaga aattggtaca tttttaagta aagaaaatgt atgtataaaa taccttataa
      421 acaatgctgg ttttgcaaaa ttctgttcat acaatgattt aagtatagat gaatctatta
      481 atatgattga tttaaatatt agtgctgttg tagcattggg acttatatgt attccatata
      541 tgcaaaaggg aagccatatc atgaatattt cttcacaagc atcattccag ccattgccat
      601 atcaaaatat ttatagttca actaaagcgt ttataagaaa ttatacaaga gcattaaatg
      661 ttgaacttaa gagtaaaggg ataaatgcaa ttgctgtatg tccaggttgg atgagtacaa
      721 atctatttaa gagaggtata gttgtagctg aaaaagggac aaaaaatttc ccaggcatag
      781 tgtctccaga cgttgttgcc aaaaaagcat tgaaggatgc aaagaaaaat aaggatattt
      841 ctgtgtatgg tataaataca aaatccagtc atttattagc aaaactattg ccacaaaaaa
      901 tgatgatgaa ggtttggtta atgcaacaac atattaagga ataagagttt tggtaattaa
      961 gtatcagttg gagattcaat ctgttatata ttatgagaag ttttgttgag caaataataa
     1021 ttgtgacaaa acggcttatc tagtgacgat aagccgtcta taatgtatat aaaatatatt
     1081 tttgatttta ctttatttag atattgaagt attaaaatat gtaggacttg aaattttata
     1141 aagataaata ctatgatatt ttgtaaaaac tgcgatatgt tgatatcaca tagtttgact
     1201 atactgttaa attgaagttt tcttattttg cattatgctt attacttaat gatttagact
     1261 ataatttcca taccatcata tgcaaaataa atgttatcaa gtttcttttc taattcactg
     1321 taataaccat atgattttcc ccacatttcg tcaatatgtg taacaataat ttttttaatt
     1381 ctataatagt ttttaatttc aacaatctca tctaaagtaa atatttcatc tttaaatggt
     1441 gtgtctttaa gttttgtacc atcatttaat ataccatcat cagaaactag acttattatt
     1501 aaaatatcag catcaaagta catatcacta tgagtaaatg gcttgggatt acagcaggca
     1561 tatatgactt ttttttcatt ctctttgata acatagaagg ttatattcct gtctgggtta
     1621 ttgttaatca aatcaatatc aaaattattt atctttacat gcgaagtttc attaatagaa
     1681 atgcaattta taacatcacc ataatattta agacaatcga gattcgttcg gtttatatct
     1741 tcaattactt caggaagcgc ataaaagcca attggattat tttctcctgt gataaagtca
     1801 catcctattt tttcgacaat tctaataccc ttaacatggt ctgggtcatg gtgagaaatt
     1861 gaaatatgtt ttacttccga tattttttgt ctgttacaag ctactgcaat gtcttcaggt
     1921 gtgtctatca gcatttttat atcatcaata tataaacttg gtcctaatcg ttcatatctt
     1981 ccgccttttt gtctggcttc ttcacatatt ttgcatttgc aaaatgaact tggaataatt
     2041 gacataccgc cactaccaac aatttttaat ttcaaagtta ttacaccacc ttctaaaaat
     2101 attagattat ataaataatc tacaatcaca actgattaac aaaattatga gttaataaat
     2161 tatatcacat gtaaatatga aagaatagta tgaaattttt ataagcatat attaaatatt
     2221 tctcaatgaa attgactttg tatatgaacc gaatgactta ttcaatatgg ataagacatt
     2281 tttcttgtaa taagatatga ttgtcctata aggtttatgt aattttgata taatttgata
     2341 taatatattg attaacaaca tacatgttat tggtgagcat atttctatag aatggaatat
     2401 ataatttttt atatttatca ttgtattatt gtacaaagaa agttaaaaag agtagagtac
     2461 aaaggaatta tttgtacttt actctttgtt ttgttaaaaa ctaatttata ttagtgattc
     2521 aaatatttaa ctagagtatg agggttgtat gtaatcttat aagtaattta tctgacaaat
     2581 taagataata tcccaatcaa attctagtca tttaaacata acatcacaag tgatttatct
     2641 gacaaattaa gataatatcc taattaaatt gtagatattt atacataaca ctaaaagtaa
     2701 tttgtttaac aaattaagat aatatcctaa tcaaattgta ggtatttata cataacacta
     2761 aaagtaattt gtctaacaaa ttaaaatatt atccaattaa tataaaaatt acttcttttt
     2821 aactgggaag catacctctg ttacccaatt atcaggatta ctatcgttgc caggacttac
     2881 atgataaata ttaaacatag gaccatccac atcatagtta ttatctgata tccaattacc
     2941 aatagcctca caagcgtctg caacttgatt gaaatttcca ttaacaattg cagttgcagc
     3001 agtcacactt ggaacagtct taaatttaac atgttcagta tctttatact ttccagatac
     3061 agcaacttgt acttcaacat ctacataatc ttctttatat ccagtatcat aaaaaactgt
     3121 tttagaatac tttgggggtt caatttgaac attctggtct tttgtctcta aaaatgcttg
     3181 atgccataac attccttcgt cttgataagt tggtataacc cttctaagtg tcatcatata
     3241 ttttggtgca aaattcttta tagttacatc ataatttttc ataatatcat ttcctccaat
     3301 tcttttaata gtggtttcta tttttaatag ttttttctgt gtatcttcta attgttcttt
     3361 tacttgagaa tggtggatat ttagatattt tatgagactt tctttatcgt tatattctgt
     3421 caaaatttct tttatagaat acaagccaaa gcccatatct tttagtgcat ggattctgtt
     3481 tgtagtagaa agttggtctg cactatagta tctatatcca ttagttttat tcgtatgaga
     3541 aggagttaaa agtcctattt catcataatg acgtaacata cgaatactaa ttttacttag
     3601 ctttgaaaaa tcgcctatct taaacatgtt tttgtctcct ttataaaggt acttttagct
     3661 taatattaat ataaaccata tcataatgtt agagtcaact aaaattataa ttaaattata
     3721 ttgacatatg atgaaaaaaa tattatactg aacatgttca gaactgaaaa tgttaattgt
     3781 tttggaggtc tattatgaaa agtaatgata aagatgctag agaacgtata attgaagtta
     3841 cattgaatct tttaaatgaa gtagatgata ttgaggagat tacagttaga aaaattgctg
     3901 agcgtgcaaa tgttggagtt ggtttaataa attatcattt taaaacaaag gataatttgt
     3961 tgagtactgc cataggagat gtgatgtcaa atataatagc agagctatat gacgatagtg
     4021 tatatactct aagacctatt gaagatttaa aaaatttatt aaaaaaattg tgtgatactg
     4081 gattacatta tgaaaaagtg cttccttttg tattgaatca atgtataacc aatggagata
     4141 tgcaagcaga acttgatatt gtccctatgc ttcgtaaaat ttttggaaat aaaaaagatg
     4201 aaatgagtct aagaatcatt gccctgcaaa ttattttacc aatacagatt tctgctttat
     4261 ctacagaatc atttcagtta tacagtggta taaatataaa gaataaatat gagcgagata
     4321 aattcattga tattttaata gaaaatatta ttggagagga tgttgatgta agatgaaaat
     4381 tataactatt ggttcaagtc ttatcactgt tttacttttt ctttcaacta tggtttgtgg
     4441 gttctggatt aagaataata aggtaacaga tgcaagttct attaagttcc atatgaatag
     4501 tgctattttt acagggatat ttttattgat ttccacaatc cttttaatta tatatataaa
     4561 aaaataataa tattattaat aaataaagta tcagaaatta taaagacaag agtcttaaaa
     4621 atttctgata ctttaccata taattttatt aaacattaat tgtgtgtgaa aaaatatcac
     4681 ttatttcaat ctaatactaa atcttaagag tgagtataaa gtattagaaa ttaagaagca
     4741 ttttttagtt caagtggatt atctatacca aaataatcat ataaagtaga gcttatttcc
     4801 tctaaatttc cattttggaa aggtaattgt aaatttacct tttctaccac atctttaaat
     4861 ccagcatcag tattttgatt taaaaaatcc atatatacat caacagcttt tctgtaatct
     4921 tcttgtgata attgccatat ctgaagtgat acatcagatg caagaccata actcaagtaa
     4981 taaaaaggaa cttggaagtt atgagataca agtatccaaa aaggagcatt tttttctcta
     5041 agtatattat tggaaactcc atattcttcc tctaaatcaa aaaacaattt attgatatca
     5101 tttagactca tatatggatt cttgtatata atttcttgaa attcatcata aagacaagca
     5161 tcaataatag tatttaaaac aatcgataag tgttcttttt ttatagcttc agattgttta
     5221 ccaaaaaaat catcaaaata tttataaaat agaatttcta aagatgttga atgaacttca
     5281 catacatcta tacttggttg catcttatta tttaagttaa tactgttgta gtaattataa
     5341 aaatgaccaa actcatgagc aaaactagta acacctaaaa aagaattgtc ccatgtatta
     5401 aacaagaaag gtgctttata tttatttata taagtagtat atcctccagg tgatttgttt
     5461 tttccagaag aataatcgta caagtcatat tttttcatat agtcgaagct ctcttttagt
     5521 tttggagata tatcttcaag tactttatca aacttcctaa atgagcgatt tttatatact
     5581 ttaatataaa tagaagaatc agatggttta gatgaaatct ctctatataa tggaactatg
     5641 taatctttta catcttgtct aaatttttta gcttcttctt gagaatagtc tttgtttagg
     5701 ttcatatagg cataatctat ataattctta aatccttgct tacttgctat ttcagttctt
     5761 gtctgtataa gttctaagaa tatgttacca aatataggat tgtatttttt taagtaatct
     5821 gaataaattt tcacatattc ttcagggcta agatttggtc ttgacaaggc ttcttcaaag
     5881 tctacctcta taccatctat tgatactgta gatttagcta ataagtcttc atattttaca
     5941 actaggtctt gctctttttt aagcaaacct tcaacttctt tagaattaag ttttccaatg
     6001 ttctttagat aattaaatgt atcatttcca agttcttttt ctaattcttt agagaattta
     6061 gaagttacaa atctatcttg aaagtctttg tatgacatgt caacatctac tgatttactc
     6121 ataagatact tatattcctt agagtaaaat tcgtctgatg aatctatgtt atttctaatt
     6181 tttgctatag taagtgtact gtaaaaatcc tgataaattt tatcaacttc attaaataaa
     6241 tttaattgtt ccttagctgt tttagctgtt aaaagtttgt cgttacattt gttaatgttt
     6301 tcgcaaattg actgtatatt aggacgctta tattccattt tttcaaaagg aatagttcct
     6361 ctaacagaat ctttttttgt atcattagta taatttctta tgtatgatgc aaatataata
     6421 aaaactgcaa ttaaaattag aaaaacaaat attttcttct tggatttaac cataaaaatc
     6481 ctccaatcat ggatatagtt attaattatt ttactatatt aaatataaaa aatgcatgtt
     6541 aagaaaattt aacataaaga tatagataag attataaata atataaaaaa tttaacttca
     6601 aaaaagtata tgaaatttat actggtaaaa tgataaataa attttaagta caaaatagta
     6661 aaagctatga tagttttaga cctattatat gtaaaaaata tagatgtttt tggtgcaatt
     6721 taaattaaaa gatatatata aaaatttaaa atattccaaa cagaaattat aaaaattact
     6781 atcattgtct ttatacaata tttatactat aatagaataa taatttagag gaaagctcta
     6841 aaaataaaca aattaagggt cttaacagta gtgattttat gttaaggagg tataaagaaa
     6901 gttgaaaaca atagttagaa gacttattct ctacgcagat ggaatgcgtc ctactcaaat
     6961 aatggtatct gggtttgctg caattattgt aatcggagca ttgttgttaa cactcccgat
     7021 agcgtcgcag agtggagaga gtataggctt attaaatgct ctatttacgg ctacttcagc
     7081 ggtttgtgtt actggtctag ttatggtaga tacagcaact tattggagtt tatttggaca
     7141 gatagttata ataacattga tacaaattgg tggtcttgga tttatgaccg tagccacaat
     7201 gttttcactt atggcacgaa aaaaaataca actaagagaa agattattaa tacaagagtc
     7261 attaaatcaa gctgatttat cgggacttgt aagacttaca agatttgtat taataataac
     7321 cataacaata gagggaatag gtgcactggt tttatcaact gtctttattc cacaatttgg
     7381 tttgtctaaa gggatatggt atagtgtatt tcacgcaata tcagcgtttt gtaatgccgg
     7441 atttgacttg atgggcagtg taagtggccc atttacatcg ttaaattctt atgtaaacaa
     7501 ctttacagta tcaatgactg tttgcgcact tatagtgctt ggagggctag gattccctgt
     7561 tgtacttgat attgtaagaa aaagaagatt ttcaaaatta aatgtgcatt ctaaggttgt
     7621 attgttttca actgctacac ttatttttgt gggtgcatta tttatttttc ttatagaatt
     7681 taatcaaaaa gctactatgg cggacttgcc tttaaaggga aaagtactat cagcaatatt
     7741 tcaatcagta acagcaagaa ctgctggttt taatacactt gatttagcta ctttacgaga
     7801 aagtagtgta tttgtaatga taattttgat gtttattgga gcatcacctg catcaactgg
     7861 tgggggtata aaaacaacaa cactagctgt tttaatcatt acagttagaa gttttctatc
     7921 aggaaaatca gatatagaag cttttgaaag aagattagct ccatcaacta taaaaaaatc
     7981 tcttggtata tttgtaatta gtatatcagc agttattttt ggaacattga ttatttcaat
     8041 aactcagcct aattttacat tagttcaatc agcttttgag gttacatcag cacttgctac
     8101 agttggttca agtttagctg gaactccaaa cttaaatgct ttaggtaaga ttataataat
     8161 aatttttatg tttatgggta gagttggttc tcttacttta tttatggcta tactttctgg
     8221 tggtagaaga aagagtcaac caatcagata tgcagaaggt aaaattatgg ttggttaagt
     8281 taccaaggag tagtataaat ttataatgga tttagagtag cactgtaaat ctgttgataa
     8341 aataaatgag cctagatgta attatatgaa gaaataaaat caactaacga acaaaattaa
     8401 ttaatgaaat aaaaaaacag ggaaataaaa tacaaataaa aaggtaaatc aaaattaaat
     8461 taatctgtgt atataaaatt atatattgtg tataagtttt gtatgtgtaa acaattaatt
     8521 taagagatat tatataaatt gtgtattagg gaggaagttt cgagatgaag caatatatag
     8581 tcattgggtg tgggagattt ggaagttcag ttgcgtctac tatgcatctt ttaggacatc
     8641 aagtaatggc aatagacaaa aatgaagatt cagttcaaag tatatctgac aaggtaaccc
     8701 attcacttat agtggatgtt actgatgagc aagcgttaag gtcattaggt ttaggtaact
     8761 ttgatgtagc agtagttgca ataggttctg atataagggc atctataatg gcgactctta
     8821 tagccaaaga aatgggtgta gagttgataa tatgtaaggc aaaggatgaa ttacaagcta
     8881 aagttcttta taaaattggt gcagatagag ttgtatttcc agaaagagat atgggagtaa
     8941 gagttgcaca caatttagtt tcggataata tattagacca tattgaactt gacccagagt
     9001 attcaattgt tgaaatcgta actccaaata gttgggttgg caagacactt atagagcttg
     9061 aattaagagc tagatatgag ataactgtac ttgctataaa aacaggtaaa aatataaatg
     9121 ttacaccttc tccagatgag gaacttacag ccggaagtat cctagttata atcggtcaaa
     9181 atactagtat aacagcgata acatctggaa ataaggggat aattagaaga agataattta
     9241 ctatttaata tatatttaat tgcaatgaaa gtagatagta tcatataatt atgaatagtt
     9301 atatgatact atttttttaa tcgaaggtag tatttatttg taagattaga taaagagaag
     9361 ttaaattaaa agtaaggagg ctgtgctaat ataaaaattt ataagttatt agcatttaga
     9421 taaaatgatt acaaatataa acagtaagga taatgaaaag ttaaagtata caagagcact
     9481 attaaaatca aagaatagga ataaagagtc aaagttcata atagagggat acagaatagt
     9541 aatgcttgca cttgaatgta tggcaaacct tgattatgta tttatcaatg aagaatttga
     9601 aaataagaaa gaacatgtaa aactattaga agatttggat aaaaaaaaca caaagatata
     9661 caagactact aataaaaact ttaaagaatt agtggataca gaaaatactc aaggaataat
     9721 aggtgtagtt tcatttaaga aaaaaaaatt aagtgaaagt ataaataaaa aagataaatt
     9781 tgtattgatt ttagacagaa tacaagaccc aggaaatatg gggactataa taaggactgc
     9841 tgattctgct ggagtagatg ccataatagc actaaaggga tgtgtcgata tatacaaccc
     9901 aaaagtaatt aggtctacta tgggttctat ttttgatatg aatataattg atgcttcaca
     9961 agatgaaact gtggacatgc ttaaatcatt ggattttaat atagtttcaa gttacttaaa
    10021 tacagaaaat ttttatgaca aaatagatta tggctcaaaa gtagcattgg tgataggaaa
    10081 cgaagcaaat ggaataaatg aagaacttgt atcaaagtct gatattttgg ttaagatacc
    10141 tatatatggt aaagccgagt cgttaaatgc tgcgataagc tctgctatac tgatgtatga
    10201 aataaaaaaa tacttaattt aatgtattgt aataaatata atgtcatgtt ataatcttga
    10261 attaaatagt agagtatcaa aaatagtcaa tatatattaa aaaaa
//
LOCUS       DAEOSP010000140        10269 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.142, whole genome shotgun sequence.
ACCESSION   DAEOSP010000140 DAEOSP010000000
VERSION     DAEOSP010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.142"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..1379
                     /locus_tag="KRH54_002661"
     CDS             <1..1379
                     /locus_tag="KRH54_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8580304.1"
                     /translation="ELDTILNLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYI
                     EDIFVDSLKVLLSEKEILDKVVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKL
                     EQNLTKKLREKGQIAKYTFKDIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKEL
                     IAQSIHNNSNRKNQPFIAVNCAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANN
                     GTIFLDEIGDMPLELQTKLLRVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNS
                     QFREDLYYRLNTIPINIPPLRERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNI
                     RELQNVTSYLNIMCEDIVLEKDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKE
                     SDVGIGRGLILKALLDKNLQITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFY
                     NKLKYKGL"
     gene            1748..2968
                     /locus_tag="KRH54_002662"
     CDS             1748..2968
                     /locus_tag="KRH54_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8580305.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKVDYIFGLHVMPHIETGFIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            3005..4354
                     /locus_tag="KRH54_002663"
     CDS             3005..4354
                     /locus_tag="KRH54_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG8580306.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            4468..5030
                     /locus_tag="KRH54_002664"
                     /pseudo
     CDS             4468..5030
                     /locus_tag="KRH54_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5138..7471)
                     /locus_tag="KRH54_002665"
     CDS             complement(5138..7471)
                     /locus_tag="KRH54_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG8580307.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIVIAVCLVTAIFGLADMAI
                     QCQKVQFIKDDGNWHIVLKNLNEEAISEISSRVDIAHYGRGQVIDNGEFKSKEILIQG
                     LDEDLAKQLGLEIIKGRYPVVENETLIDVDAVKSFNLKVGDFIKIKLADGRNKNYKIV
                     GEYSSLSSLRASDKHGLMLSILGTQAIKKSETYDYYYITFKDKVNIRKATSDIKNTFN
                     LSDKQIGKNEKLLSIIGQGDSDYSKPLYLTAGGLFILVLIASIIMIYNSFNMGVIERI
                     KFFGLLRCLGASKLQVKKFVVLESLILSVKAIPLGLLLGCIVTIISSIFLKYVNTELF
                     GSMPILKVSFIGIVFGIIVGFLTVILSAIVPAKKASGVSPLSAIRGNLMFKEILTSGK
                     ASKIANAGSSKIDVSMGINHAFSRKKSFILMTSSFAISIVLFLGFSVFIDFMYQALKP
                     IKPEAAHISIVDADFKSVLTKDDINEISNIKGIKKVYSRMMTDVEATYNNKEATSQLI
                     SYEKNQFDWAKKYLVKGEINEDKLESENIGLVEEGHGFNIGDKIYIKNGNSKKEIEVA
                     GILSSISFDVKDKYIGNIITSENTFTSITDIKDYIIIDTQVDEDAPEDLIQNIRNVLD
                     DNLEVRDKRQGNLEAKNSFYTMAVFVYGFVFIIAIISMFNIINSMNISVTSRINYYGI
                     MRAIGMSNKQLRKMVIVESSTYAVSGCLLGSVLGLILHRYIFVSLVTLKFHIDWQIPF
                     DLLFIIVVTMIIITLLAVRKPIKKICELDIIEVVNAQ"
     gene            complement(7468..8151)
                     /locus_tag="KRH54_002666"
     CDS             complement(7468..8151)
                     /locus_tag="KRH54_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580308.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGKIFVDGKEICSMKEEKQTIFRRRNIGFIFQSYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKSHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSSDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGM
                     LKDLGVNRE"
     gene            complement(8222..9463)
                     /locus_tag="KRH54_002667"
     CDS             complement(8222..9463)
                     /locus_tag="KRH54_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8580309.1"
                     /translation="MKVFSNKDIKIFFLVIISVLLMFIVVGQIVVINITNDYKSALLE
                     HDYNIAGYLNSMGVDKSKIPAVFTTNEDKYTLKGKEILNTARYDFGTDDSFMPSVKLF
                     QGKYIKTMFLYLFVFFTMIIFILYVYFFKQQNKIEEASYKIDSFMNGNINVRLNTYEE
                     GSLSKLFGSIDAMSTSLNTHILKEKQNKEFLKNTISDISHQLKTPLTALMMYNQILQE
                     ESHNSEIVENFVQKSQNELERIESLIQNLLKITKIDSKTIILNKASVNVKKLITKILY
                     SFETRAEKEGKSISIRGLENTNLFCDYEWISEALSNLIKNALDNTKENDKIMIEWVET
                     PITTIISVSDTGNGIHIEDIHHIFKRFYRSKFSKSNQGLGLGLPLVKSIIEQHNGTIT
                     VESNFLQGSTFTLSFLKLTNM"
     gene            complement(9474..10160)
                     /locus_tag="KRH54_002668"
     CDS             complement(9474..10160)
                     /locus_tag="KRH54_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8580310.1"
                     /translation="MSSILLVEDDLSLIYGLEYSIQKNGFSVDVARTVEEALQIYKEK
                     NYDLLLLDVSLPDGDGFDICKRVREASNVPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNFTSENVTELKSNNITVKLLENRVFKNEIELELTTAEYKLL
                     CLLMKNKNIVLTRKNILDKLWDGNGSFVDDNTLSVYVRRLRNKIEDNPENPKILLTVR
                     RMGYKWNVIK"
BASE COUNT         3744 a         1497 c         1335 g         3693 t
ORIGIN      
        1 aagaattaga tacaatattg aatttatcaa aagatggtat attatttact tcaaaagatg
       61 gtgaaataaa tacatataat agtaaagtaa aagatatttt agacataaat gaggatatat
      121 atggaaaata cattgaggat atatttgtag atagtttaaa agttttattg agtgagaaag
      181 agatactaga taaagttgtc gtattcaata agaaatatat aaatgtaaat aaaaaaaaca
      241 tatacaatag agatgaaaaa atgggcacat attatagttt acaagaaata acatacataa
      301 aaaaattaga acaaaatctc actaaaaaat taagagaaaa aggacaaata gcaaaatata
      361 catttaagga tataaaaaca aatagtccta aaatgtttga atgtatagat ttagctaaga
      421 aagtttctaa atctgattta agcatactta tcagaggtga aagtggaact ggtaaagaac
      481 tcatagctca gtctatacat aacaactcaa atagaaaaaa tcaacccttt atagcagtaa
      541 attgtgcagc cgttcctgaa aatttattag aaagccagct attcggatat gacaagggaa
      601 cttttactgg aggtctaaaa gatggaaaac aaggattatt tgaacttgct aataatggaa
      661 caatattctt agatgaaata ggcgatatgc ctttagaatt acaaacaaaa ctacttagag
      721 ttcttcaaga gaaacagata atgcctgttg gttctcacaa tgtgattaat atagatgtta
      781 gaatcatatc tgctacaaat aagaatttag aacaaatgat tgataatagc caatttagag
      841 aggatttata ttatcgatta aatacaattc caataaatat acctcctcta agagaaagaa
      901 aagaagacat attaattatt atggaagatt taattaataa gaaattagta atcacaccag
      961 aagcaaaaaa acttatacaa aactatatgt ggaaaggtaa tataagagaa ctccaaaatg
     1021 ttacatccta tttaaacatt atgtgtgaag atattgtatt agagaaagat ttacctccaa
     1081 acttaaggtc ttcagataac aaaaatacat cattaaaact aaaatatagt aagaatgata
     1141 tattgaatat attagagata ctaattttaa ataaggaatc agatgttggt attggtagag
     1201 gattaatttt aaaagcacta ctcgataaga accttcaaat tacagaagga aaaattaaaa
     1261 aaatatttga atatctaaaa aaagaagaac ttataatttg tagttcagga agatatggaa
     1321 gtaaaataac tcaaaaagga gaagattttt acaataaatt aaaatataaa ggtctataaa
     1381 attataattt atcaaacaaa gaaatctatt caggattaaa gtactccttc aataggtttt
     1441 tttatttttc aagtacccta aaattataat ttattttgta cataaataat aggaattaat
     1501 aggaatagga taattttttt atttcctatt ttttttatat aaactagcat ataaaatata
     1561 ttttcttcaa aataatattt tttactgaaa attgaaacta tgcaatcatt ttttatacta
     1621 tacatataga taaatgatta tatttattca atattcttat aaaaaacaaa gttctccatc
     1681 gaaaattatg gcataataat tgcttattat attaataaaa caataaaaag caattggagg
     1741 aataaaaatg caacaggctt tgaaattaaa atatcaaacc aaagaattag gaaaagattt
     1801 tgtaatagaa agttgtaatt ctataaaacc atggcttata aacataagaa gagaattaca
     1861 taaaattcca gaattagctc ttgaagaaaa tcttactaaa cagaaggtaa tttcttattt
     1921 aaaagaaatc ggaattgatt atatggaatt tacaaaacac aatggaatta tggcatatat
     1981 tttaaaagaa agtgcagata aaacgatttg tataagagca gatatggatg ctcttcctat
     2041 tgaagaagaa aacaacatac cttataaatc aatacattct ggaaaaatgc atgcttgtgg
     2101 ccatgatgca catacaacca tgctacttgg agcatgtaaa gttttacatt caataaaaga
     2161 taaacttaat gtaaatgtaa aattcttatt tcaaccagca gaagaaggtt ttggaggagc
     2221 aaaattctta gttgaagatg gatgtttaga aaatcctaag gtcgattata tattcggact
     2281 tcatgttatg ccacatatcg aaacaggatt tatcgaaaca aaatatgata cattaaatgc
     2341 tagtgtagac actataaaaa taagtatcaa aggtaaaaga gctcatggtg catatcctga
     2401 aaatggtatt gatgcaatag ttacagcttc acaaattgtt acatcattac aaactataat
     2461 aagtagaaac ttagaaccaa ataatgctgc tgttttgact ataggtaaaa tatatggtgg
     2521 tgatgcacac aatgtaatat gtgaagatgt aaaactagaa ggcactttaa gaaccttaaa
     2581 tagcaaaaca agaaacttta tgattgataa aatagctaaa attgttggag acactgcatc
     2641 tgcatttggt tgtgttggga ctttacatgt aagtgatgaa aactatcctg ctgttataaa
     2701 tgaaaaagaa ttagtagata cagttatatc aaatactaaa gaattacttg gagaggaaaa
     2761 atttatattg agaccaaacc cgtctttagg tggtgaagac ttttcttttt acacagaaca
     2821 ttgtaaagga gcattcttcc atttaggatg taaaaatgag gaaaagggct taatttcacc
     2881 tcttcatacc tctagcttta atatagatga agattgttta ccaataggtg ttatgatgca
     2941 tgtaatgaat actttgtatt ttaattagta cagtaattaa taaaagaata ggagataaat
     3001 taatatggaa aaagctaaga aattaggtct atttagtatg atattattag ggattaatag
     3061 cataataggt tcaggtatat tcttactacc aggtaaagtt tataatctag caggtcaaaa
     3121 cagtatgttt atatacattt ttgctacact cttagttcta tcaatacttt tatgttttgc
     3181 agaagttgga agcatgtttg ataagaatgg aggagcatat ctatactcca aaaaagcttt
     3241 tggagatttt ataggatttg aagttggtac aatgtcttgg gttattagaa ttatatcatg
     3301 gtctactcta gcagtaggat ttgcaacagc tttaggttca ttttggccag aatctgccac
     3361 tgaatacaaa ggttatatag ctgcaatact cgtaacacta ctttcaataa atagcttatt
     3421 tggaattaaa agcacaaaga taatgaataa tgtaataact atagctaaat tagtaccatt
     3481 aatagttttt ataatagttg gaatattctt tataaaattt gtaaatattg ttccttcagg
     3541 aaatgtagtt aactctagta tgggtccagc cattatatta gtattttacg catttaccgg
     3601 ctttgaatca tttattgtgg caagtggaga aatggaaaat cctaaaaaaa atcttccagt
     3661 cgcattaata accacaatat ttatatgtgc aataatttat atattaattc aaattgtctg
     3721 tatgggtata cttggagata gactatttga aaacagtatt ccaattgcag atgcttcaag
     3781 tgtattttta ggcaattatg gtaaagtttt catatctgta gcaacattaa tatctatatt
     3841 tggtataaat ataggttcat caatagtaac tcctaaatgt ggttcttctc tagcagaaga
     3901 aggttctctt cctgcattta taggaaaaac aaataaatat ggtgctccat atgtagcaat
     3961 aataatatct ttaatttgtt gtataccact tgtgctaact ggaagttttg aacaacttgc
     4021 agtaatgagt gtaattgcaa gatttgctca gtacatacca acttgtttat cagtaatagt
     4081 tttaagaaaa agaacagatg ttaaggcctc ttttaagatt ccatttggtc ctgttatacc
     4141 aatggtagct attttaggta gcttatggtt gttacaacaa gcttgggcag aagatatttc
     4201 aaaacctata actcaaaata gagttttaat aggattaggg gcaatgttgt taatagctcc
     4261 tctatatata tttatgaaaa aaaataagga aatcgagcat aaaaataaat cagaagattc
     4321 taaattatat atttcgagag aaaaaattca ataatatttt acaaactatt taaaaattat
     4381 ttttctaata ctatagaaat aattagaaat ggagatgtta cgtatctaaa agtagttgat
     4441 aacacggata ttggtacttt tatagatatg ggacttacaa aagacatact agttcctttt
     4501 aaagctaaga cttatccatt gtttagagat gaaaaatatt tattctatat atacttggat
     4561 aaaagcggaa gaatagctgc tactacagac atagattcat atttagaaac agaccatact
     4621 tacaatgtag gagatgttgt aaatggagtt gtatatggat tccaaaccaa caactctgct
     4681 atgatatgtg ttgacaataa atatgctggt gtaatccttc ataatgaata ctttacagaa
     4741 ttaaaagctg gggatgttct tgaaaattta catgttataa aaatatacga tgatggtaaa
     4801 ttaggacttt ctcctagagg aaacagaaaa gatgaacttg ataccttaga aaataagatt
     4861 ttaagctatt tagaaggttc agatggatat atgagattca atgataaatc tgatcctaaa
     4921 gatatttcta tcttatttaa ttcaagtaag aaaattttaa acgtgctctt ggagtactta
     4981 tgaagaaaga cttaatttat caagatgaag atggaactta tctaaagtaa attatataac
     5041 acaaaaatcg aagtaaatta tgaaagatat tttctatttc atatttactt cgatttttta
     5101 tttaaattac tacctctatt taaattacta cttatattta ttgtgcattt actacctcta
     5161 ttatgtctaa ttcacaaatc tttttaatgg gttttcttac tgcaagcaaa gttattatta
     5221 tcatagttac tacaatgata aacagtaaat caaaaggtat ttgccagtct atatggaatt
     5281 ttaatgttac taaggataca aatatatatc tatgtaatat taatccaagt acactcccca
     5341 ataaacagcc acttacagca tatgtacttg attctactat taccattttt cttaattgct
     5401 tattggacat gcctattgca cgcataatac catagtaatt tattctactt gtaacgctta
     5461 tattcatact gtttatgata ttaaacatac ttataatcgc aattataaat acaaacccat
     5521 atacaaaaac tgccatagtg taaaatgaat ttttagcctc taagttacct tgtcgcttat
     5581 ctcgaacctc taaattatca tctagaacat ttcttatatt ttggattaaa tcttcaggag
     5641 catcttcatc aacttgagta tcaattatta tatagtcttt tatatctgtt atactagtaa
     5701 aagtattttc agatgttata atatttccaa tatatttgtc tttaacatca aatgaaattg
     5761 aagaaagtat acctgctacc tctatctctt tcttagaatt tccattttta atatatatct
     5821 tatctccaat attaaaccca tgaccttctt ctaccaatcc gatattttct gactctaatt
     5881 tatcttcatt gatttcccct tttactagat actttttggc ccagtcaaat tgattttttt
     5941 cataagaaat tagctgagat gttgcctcct tattattgta tgtagcctct acatccgtca
     6001 tcattctact gtatactttt tttattcctt tgatattact tatttcatta atatcgtctt
     6061 tagtaagaac agacttaaag tcagcgtcaa caatagatat atgagcagct tctggtttta
     6121 taggtttaag tgcctgatac ataaagtcta taaatacact aaaacctaaa aacaatacaa
     6181 tactaatagc aaatgaagaa gtcataagta taaaagattt ttttcttgaa aaggcatgat
     6241 ttattcccat agaaacatca attttagaac ttcctgcgtt agcaatttta cttgcttttc
     6301 cactagttaa aatttctttg aacattagat taccacgtat agcacttagt ggagacaccc
     6361 cagaagcttt ttttgctgga acaatagctg ataatattac tgttaaaaat cctactatta
     6421 ttccaaacac aataccaata aagctaacct ttaaaattgg catcgaacca aataactctg
     6481 tattcacata tttgagaaat atagaggata taatggttac aatacatcct aataacaatc
     6541 ctagtggaat agcctttaca cttagaatta aactttctaa aacaacaaat tttttgactt
     6601 ggagttttga tgctcccaag caacgtaata gtccaaaaaa ctttattctc tctattacac
     6661 ccatattaaa gctattatat atcatgataa tacttgctat taacacaagt ataaaaagtc
     6721 ctcctgctgt taagtaaagt ggtttagaat aatcagagtc accttgacct ataatagaaa
     6781 gtaatttttc atttttaccg atttgtttat cacttaaatt gaaggtattc ttaatatcag
     6841 aagtagcttt tctaatatta accttgtctt taaatgtaat ataataataa tcatatgttt
     6901 ctgatttttt gatagcttgt gtacctaata ttgaaagcat taatccatgc ttatctgagg
     6961 ctcttaagct tgataatgat gaatattcac ctactatctt ataattttta tttctaccat
     7021 ctgctaattt tattttgata aaatctccta cttttagatt aaagctttta actgcatcta
     7081 catctattaa agtctcattc tccacaacag gataacgacc ttttattatt tctagaccta
     7141 attgttttgc taaatcttca tctaaacctt gtatcaaaat ctcctttgat ttaaactctc
     7201 cattatcaat gacttgtcct ctaccatagt gagctatatc tactctacta cttatttctg
     7261 atatagcttc ttcattaaga tttttaagta ctatgtgcca atttccatca tcttttataa
     7321 attgtacttt ttgacattgt atagccatgt ctgctagtcc aaaaatagca gtaactaaac
     7381 atactgcaat gacaatacat aaaattgtag ttctattttt tttcttatgt acctttgcat
     7441 attgtggtac tagttctaaa tagcttctca ttctctattc acccctaggt ctttaagcat
     7501 tccatcagaa acctgtagta tcctatctgc tgtagaagct atatttgtat tatgagttat
     7561 cataataata gtttggttat atttttctgc tgtcactttt aaaagtgaaa ttacatcact
     7621 gctattttta ctgtcaagat ttcctgttgg ctcatctgcc aatataattg atggctttgt
     7681 aatcaatgct cttccaattg caaccctttg ttgctgacca ccagataatt gatttggaag
     7741 atgacttttt ctatctttaa gacctaaaat tgtcaatact tcatctagat aaatagtgtc
     7801 tggttttttg taatctagta gtaaaggaaa tattatattt tgttccacag ttaattcggg
     7861 tataagatta tatgattgaa agataaaacc tatattacga cgtcgaaaaa tagtctgttt
     7921 ttcttctttc attgaacata tttctttacc atcaacaaat atttttcctg aggttggagt
     7981 atcaagacct cctaaaagat ttaataatgt actcttacct gagccagatt ctccaactac
     8041 agtaacaaac tctccctttg aaactgtaaa tgatacatct tttaatgcct ctacttttgt
     8101 atatccattt ccatatgttt tacataaatt attaaccaca agaatatcca tttgctctat
     8161 ctcctttctg atgattaata taagaataat tcatacacct tactgtaaga tgtatcctat
     8221 cttacatatt agtaagcttt aaaaaactta gagtaaatgt acttccttgt aaaaaattac
     8281 tttctacagt tatggttcca ttgtgctgtt ctatgataga tttaactagt ggcaacccta
     8341 atccaaggcc ttgattactt tttgaaaact tactcctata aaatctttta aatatatgat
     8401 gtatatcttc aatatgaatt ccatttccgg tatcactcac tgaaataata gttgttatag
     8461 gtgtttcaac ccattctatc attattttat cgttctcttt tgtattatct aatgcatttt
     8521 ttataaggtt gcttaaagct tctgatatcc attcataatc gcaaaacaag tttgtatttt
     8581 ctagacctct tatagagata ctctttcctt ctttttctgc tctagtttca aaactatata
     8641 aaattttagt aatcaacttt tttacgttaa cactagcttt gttaagaatt attgtctttg
     8701 aatctatctt tgtaatcttt aacaagtttt gtatcaatga ttctatacgc tctaattcat
     8761 tttgactctt ctgcacaaaa ttctcaacaa tttcactatt atgactctct tcctgcaata
     8821 tttgattata catcatcaat gctgtaagtg gagtttttag ttgatgtgat atatctgaaa
     8881 ttgtattttt aagaaattct ttattctgct tttctttcaa tatatgtgtg tttagagatg
     8941 ttgacatagc atctattgaa ccaaataact ttgataaact accttcttca taagtattta
     9001 accgtacatt tatatttcca ttcataaatg aatcaatttt ataactagct tcttctatct
     9061 tgttctgttg cttaaaaaaa tacacataca atataaaaat aatcatagta aagaacacaa
     9121 ataaatacaa aaacatagtt tttatatatt taccttgaaa caatttcaca cttggcataa
     9181 aactatcatc tgtaccaaag tcatatctag ctgtatttaa gatttctttt ccttttaaag
     9241 tatatttatc ttcattagta gtaaatactg ctggtatttt tgatttatca acacccatac
     9301 tatttaagta tccagctata ttataatcat gttctaacag tgcactttta taatcattag
     9361 ttatatttat aactactatt tgacctacta ctataaacat caataaaacg gatataatca
     9421 ccaaaaaaaa tatttttatg tctttattag aaaatacctt catattaaca ccctcacttt
     9481 ataacattcc atttgtaacc cattcttcta actgttaata gaatttttgg attttcagga
     9541 ttatcttcaa tcttatttct taaccttctt acatatactg acagagtatt atcatctaca
     9601 aaactaccat ttccatccca taacttatcc aatatatttt ttcttgtaag tacaatattt
     9661 ttatttttca taagtaagca taataattta tattctgctg tagtaagctc aagctcaatt
     9721 tcatttttaa aaacacgatt ttcaagtaat ttaacagtaa tattatttga ttttagttca
     9781 gttacatttt cacttgtaaa attataacgt ctaagtaaag cttttattct tgaaattaac
     9841 tcatttaatt taaaaggttt tgttatgtaa tcatctccac ccatatcaag tcccatcact
     9901 acatttactt cttcatcaga agcagttaaa aatatgattg gtacattaga agcttctctt
     9961 acccttttac aaatatcaaa accatcacca tcaggtaagg atacatccaa aagcagtaaa
    10021 tcataatttt tttctttata aatttgcaat gcttcttcta ctgttcttgc tacatcaaca
    10081 ctaaatccat tcttttgtat agaatattct aatccatata tcaaacttaa atcatcttca
    10141 accaacaata tactactcat ctcaatcact tccatttttt tattttttac tttatatata
    10201 ttatatacct aacttattat atttttactc ttttaaaaat acaatacctt gaaatcaatg
    10261 atttaaata
//
LOCUS       DAEOSP010000141        10170 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.143, whole genome shotgun sequence.
ACCESSION   DAEOSP010000141 DAEOSP010000000
VERSION     DAEOSP010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10170
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.143"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(21..155)
                     /locus_tag="KRH54_002669"
     CDS             complement(21..155)
                     /locus_tag="KRH54_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBG8580311.1"
                     /translation="MWELLKDFLLVSLGMGIGVVLMCILTVGKEADRCMEEIKEREEH
                     "
     gene            complement(162..1361)
                     /locus_tag="KRH54_002670"
     CDS             complement(162..1361)
                     /locus_tag="KRH54_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBG8580312.1"
                     /translation="MNETDFLTALKAKRCAYSVSQTRLAIMANISREHLSRIEAGKVT
                     LTEDMKHTLLEAVEKFNPDNPMFLLFDYVRIRFPTMDIKHIIKNILKLNIDYMLHEDY
                     GHYKYTEHYHIGDVFVYVSQDEEKGVLLELKGKGCRQFESYLLAQERNWYDFFTDALV
                     EGGVMKRLDLAINDRTGLLDIPELIQKCENEECISKFRSFKSYGSGELVKHNETDKYG
                     MGHTLYIGSFSSEVYFCCYEKNYEQYAKLGIPIEEVPIKNRFEIRLKNERAYYAVVEL
                     LTHYDAEQTAFSIINQYIRFADKEPDKRKSDWKTNVRWAWFIGEGRPPVKLTTKPKPY
                     TLERTLNWLQRQVAPTLKMLKQIDKDNQTEYLETIEKQAKLTERHHQIIKQQTATVEE
                     MVAKVKE"
     gene            complement(1563..2957)
                     /locus_tag="KRH54_002671"
     CDS             complement(1563..2957)
                     /locus_tag="KRH54_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8580313.1"
                     /translation="MRIIWNKGHRIRASDKNLVYRFSLGTLLIVFVVVLLLLNMKQLM
                     RTDWEHFSLLDNSFTLSLYNFITLLIATGVCALVAFLYYRFCYDNFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIKYILLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKQHPNYKTGENYAYLSLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSIISEFYTPLVPKGYDFLQTIGSLAYARQ
                     DGTATCEAKGDGTD"
     gene            complement(3068..3379)
                     /locus_tag="KRH54_002672"
                     /pseudo
     CDS             complement(3068..3379)
                     /locus_tag="KRH54_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896960.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
     gene            complement(3407..3949)
                     /locus_tag="KRH54_002673"
     CDS             complement(3407..3949)
                     /locus_tag="KRH54_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG8580314.1"
                     /translation="MKGFERKNQLFSLCGLNCGLCPMLLGNYCGGCGNGNQSCRIAKC
                     SLEHGQIEYCYECKQYPCEKYQHIDDYDSFITHKQRKADLERAKNIGIEQYNLEQQEK
                     TQILSYLLSNYNDGRRKTFFCVAVNLLELSELQEAMKQIQSNDELTLLPFKAQCLYVV
                     EVFQKIAERRNIKLKLFKKK"
     gene            complement(4010..4612)
                     /locus_tag="KRH54_002674"
     CDS             complement(4010..4612)
                     /locus_tag="KRH54_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8580315.1"
                     /translation="MDKVVIYIHGKGGNAEESSHYKPLFSDCDVVGLNYTAQYPWEAK
                     EEFPSLFDLICKHYKSVQIVANSIGAYFAMNALSDKQIEKAYFISPIVNMEKLIADMM
                     YWANVTEDELRDKKEIKTTFGETLSWEYLCYVKENPTTWKIPTNILFGEKDNLTSYAT
                     ISEFTKQTNATLTVMRNGEHWFHTEEQMKFLDEWINKSAR"
     gene            complement(4667..5080)
                     /locus_tag="KRH54_002675"
     CDS             complement(4667..5080)
                     /locus_tag="KRH54_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG8580316.1"
                     /translation="MLDKIPNAEEMTALVGQSLYAIWNKLCALIDEKYDMECLWNKGG
                     KAWTYEYKYRRGGKTLCALYARENCVGFMIILGKDERLKFDTDRNSYSKEVQKIYDET
                     KTYHDGKWLMFEPTDTILFNDFVKLLGIKRKPNRK"
     gene            complement(5144..5602)
                     /locus_tag="KRH54_002676"
     CDS             complement(5144..5602)
                     /locus_tag="KRH54_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG8580317.1"
                     /translation="MKFKNPMLVVTDIDKSVEFYKKVFGLRVIMDFGANKTLTGGLAL
                     QTSETYKEFIGTSDISFGGKNFEVYFEEDDFDKFADRLKEYDIEYVHPIIEHSWGQRV
                     VRFYDPDKHIIEVGENMKIVCKRFLNSGMTPEQVAERMDVPMKFINACMR"
     gene            complement(5683..6096)
                     /locus_tag="KRH54_002677"
     CDS             complement(5683..6096)
                     /locus_tag="KRH54_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG8580318.1"
                     /translation="MRLANGIVLDNEKTFGVLKFSALRHEVRVENEDGTTSDEIKRRT
                     YDLKCSVQERMIQVSIPADVPVKDFPYNAEVELINPVADTVANANFRGADVDWYIKAD
                     DIILKNKGNQPAGNPQNNNPQNLAPQGQQKNKLAN"
     gene            complement(6111..6434)
                     /locus_tag="KRH54_002678"
     CDS             complement(6111..6434)
                     /locus_tag="KRH54_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBG8580319.1"
                     /translation="MELKYIVPNVAQTFGNLEFAGENKVDQQHINGRYTPVSRSYNLY
                     SDIQRADDIIVVLPAKAGEKHFKPEQKVKLINPRITAEGYNVKGRGFTNYILMADDML
                     PAESK"
     gene            complement(6656..9703)
                     /locus_tag="KRH54_002679"
     CDS             complement(6656..9703)
                     /locus_tag="KRH54_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBG8580320.1"
                     /translation="MKKILKRLCTGFLAFATVVTALPSSTVHASNTQYWTESKERVGI
                     VEQVMNDGSISSTFNEGHLTVEGKDAYCIDINTAFKNGYKTRTDASSRMSADRISDVA
                     LSIEYVKQYTDSHSGISSKHAYLLKQLVVWQRLSVQLDWQCDNVRASYDEIPKAVQDE
                     VFAGAKAFVKENKGRYDCGGYIYSGEGQELGQFWAKLAVGNAKLQKTSTNANITDGNG
                     IYSIAGATYGVYSDKDCTKQLATLTTDTSGNTEAVEVRATTVYIKELSAPAGFKIDKT
                     VYSLSVEAGKTATLKVSDTPKVTDTLIELFKIDMETQKSNSQGNASLEGAEFTWNFYA
                     GYYNKNNLPAQPTRTWVTKTIAEKDSDGAIHYITRLANAYKVSGDSFYMQDGKNVLPL
                     GTLTVEETKSPSGYLLEGAYMQADGSEEQIKEMYLTQITEDGDLAVLSGSNQYHVSDK
                     VIRGGVKIQKRDLETKDTKAQGGATLKNTAFEIISLNENAVLVESKLYKKNEVLKTIH
                     TDIEGIASTSADLLPYGKYRLSEQKPPEGYLTDGAKPIDFEITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGSHKRLADVPFRITSKTTGESHVVVTDDNGQFSTASDWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALIYDTYIIEELRCESNKGFKLIPPFEIVISRN
                     KVVVDLGTLTDEYEKEITIHTTATSKDGEKTILAGNDVTIIDTVKLDGLVKGTKYQLK
                     GWQMLKKENAELLVGDKRVESDYTFVADDEAMKVEIAYTFNASALGGKNLVTFEELYD
                     LSNPEEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGKKEIEAGKDVTIVDTVK
                     LEGLEVGTKYQLVGWQMLKEENAELIINDKKVENDYTFTSDSETMEVKIAFTFDASSL
                     GGKQFVTFEELYDLSNPDKPIKVTEHKDIEDDGQTVTIKEVPETPTPEEPEKPTTPDT
                     PTKTDSPKTGDNTNILAFAIMMFVSAGGLAGTYFFKRRKMKKS"
BASE COUNT         2713 a         2167 c         1770 g         3520 t
ORIGIN      
        1 aaattttgtc caaaattcat ttaatgttcc tctctttctt tgatttcttc catacatctg
       61 tcagcttctt tgccgacagt taaaatgcac atcaagacta cgccgatacc cattcccaga
      121 gataccagca ggaagtcttt caataattcc cacatttttc atcactcctt tactttggca
      181 accatttctt caaccgtggc tgtctgttgc ttgattatct ggtggtgtcg ctcggtcagt
      241 tttgcctgct tctcaatcgt ttccagatac tccgtctgat tgtccttgtc tatctgtttc
      301 aacatcttca aggtcggtgc aacctgcctt tgtagccagt tgagtgtacg ttctaacgtg
      361 tacggttttg gctttgttgt cagctttacg ggtggtcgcc cctcgccaat gaaccaagcc
      421 cagcgtacat ttgttttcca gtcgcttttt cgcttgtctg gttctttatc agcaaagcgg
      481 atatactggt tgataataga aaatgctgtc tgttctgcgt cataatgtgt cagcagttcc
      541 acaacagcat aataggcacg ttcatttttc aaacgtatct caaatctatt ttttatgggg
      601 acttcttcaa tcggtatgcc aagttttgcg tactgttcgt aattcttttc ataacagcag
      661 aagtatactt cacttgaaaa tgaaccgata tacaacgtgt gtcccatacc gtatttatcc
      721 gtttcgttat gctttaccag ttccccagaa ccatagcttt tgaaagaacg gaatttagaa
      781 atacattcct cattctcgca tttttgtatc agctctggaa tatcaagtaa acccgttcgg
      841 tcattgattg ccaaatcaag acgtttcatc acaccgccct caaccaatgc gtccgtgaag
      901 aagtcatacc aatttcgctc ttgtgccagc agataacttt caaactgacg acagcctttg
      961 cctttgagtt ctagcaggac acctttttcc tcgtcctgcg atacatacac aaacacatct
     1021 ccgatatgat aatgctcggt gtatttataa tgtccgtaat cttcgtgaag catatagtcg
     1081 atattgagct ttaatatatt cttgatgata tgctttatat ccattgtggg aaaacggata
     1141 cgcacataat caaacagcag gaacatggga ttgtcgggat tgaatttttc caccgcttct
     1201 aaaagcgtat gtttcatgtc ctcagtcagc gttacctttc ctgcttcgat acggctcaaa
     1261 tgttcacggc tgatatttgc catgattgca agccttgtct gcgatacgct gtatgcacag
     1321 cgtttcgctt tcaaggcggt taaaaaatca gtttcattca gttgtcatac ccccaatcgt
     1381 gataattttg tgatattgtg ataatttcaa ggttttatca caaaaggcga gatgtacgaa
     1441 aaaccctcat tccatgcggt cttgcaaagg tttttgtgga agtttccgcc gtaattgtgc
     1501 ccctctgtta gataatgagg ggcttgtttc tgctggtgtg gcgttagcca caccagcaac
     1561 ggctagtccg tgccgtcgcc tttcgcttcg cacgtcgccg tcccgtcctg ccttgcatat
     1621 gcaagagaac ctatggtctg caaaaaatca tatcctttcg ggactaaagg cgtgtaaaat
     1681 tcacttatga tgcttgttcc cacatcacaa tagccacgcc ctttgatacg cttctggaaa
     1741 aactgttttt tcacatcaga accaaacagc ataccgtaac ctaattcgct gatacgtccc
     1801 aaccctacac ggaaattgaa gttatctctg ataccgtccg agaaatattt tgcgtctggt
     1861 cgctggcagg caacgataag gaaataccct gcttgtctcc ccagcatgac gattttcttt
     1921 aactggctaa gtaaactcac gctttccttt gtccccagca tttcaaaaaa cgccacatat
     1981 tcatcaaaga ttaaaaagca gggcggtaag ctaagatagg cgtagttttc tcctgtttta
     2041 tagttcgggt gttgcttcat ttcttcgctt cgttgtacca tgccctcata aaaggcattg
     2101 acgcaatcaa tcatttcttc tttagtgtga tacacgtttc ccatgactgt tcccaaatct
     2161 gcaaggtcag cgttctttgg gtctaagatg taaaggacag cgttggtatg cagtagggct
     2221 tcaatgagcg tcagaagaaa ataggtttta ccgccaccag tcccaccagc aatcagagcg
     2281 tgagggagtg cgtcatattc ccagacaaga tttttcatca gtctaagaca gccgttttct
     2341 gcccgtactt catcaatagt aatgcggttt gctatcatgt cataaagcag gatatattta
     2401 atatagccgt catgtaaggt cttgtcggtc agctcgcaat acaagccact ttccaattta
     2461 tcctctaacc gtaaaagttg gtcttgatat tttcctaatg tgatttcaca gcggatatga
     2521 agcagtccct tttccatttg ataataaatc tttggaaacc agacgatttt ttcccttgat
     2581 ctgctttgca ggtcagtaaa aaaaccgctg tcttgtacgg tatcggcttc ataccactta
     2641 ttttccagta tcattcgtgc cagcttttga cggtgcagta gctttttaaa attgtcataa
     2701 caaaagcggt aatacagaaa agcgaccaat gcacaaacac cagtcgctat cagtaaggtt
     2761 atgaagttgt ataaggaaag cgtaaagctg ttgtctaata agctgaaatg ttcccagtcg
     2821 gtacgcatga gctgtttcat attcagtagc agaagaacca ccacgaatac aattagaagc
     2881 gttcccaatg aaaagcgata gacgaggttc ttatcgctgg ctctgatacg gtgtccttta
     2941 ttccaaatta ttcgcataaa tcaatcactc caatcttaaa tttaagtatg aaaaaagcag
     3001 tcaatcttaa aagactaacc gctttgtgtg tatgaatatg aagttgttac attgtagggt
     3061 atgttcatta tatatgcttt gttgttttaa ctcttgattg tgcaatttct tcaaatagct
     3121 gtttttcgtt taggtcttta aaaactcttt tgggaattaa cgtatataat cgtggtgctt
     3181 gaattaagta ataaaaatca tggctttccc atagtgcaga gtaaatgtcc cacttcactt
     3241 ctgagtcaat gctttgtgtt ttaaatttaa tgttttcctt agaaaatacc agcgtatatt
     3301 cctcatgata ttttgcagtt tgtttgaatt tcagtatagg cattaggata tataaatagc
     3361 ttcccaatgc agttacaact ctttgctaca atttcaaatt tgaaaatcac tttttcttga
     3421 aaagtttcag ttttatattt ctcctttcag caatcttctg aaacacctcc accacataca
     3481 agcattgtgc cttaaatggt agtaaggtca attcgtcatt ggattgtatt tgtttcattg
     3541 cttcttgtaa ttctgaaagt tctaataaat tgactgccac acaaaagaaa gtctttctac
     3601 gcccgtcatt atagttagaa agtaagtaag aaagaatttg tgttttttcc tgctgttcaa
     3661 gattatattg ctcaatgcct atgttttttg ctctttctaa atctgctttt cgctgtttat
     3721 gcgtgatgaa agaatcataa tcatcaatgt gttgatattt ttcgcatgga tattgtttgc
     3781 attcatagca atattcaatc tgtccatgtt caagactaca ttttgcaatt ctgcatgatt
     3841 gattaccatt gccacaaccg ccacagtaat ttcccaaaag catagggcat agtccgcaat
     3901 tcaatccgca aagagaaaat aattggtttt ttcgttcaaa tcctttcata gtaccagtct
     3961 cctaacttca aatttatctt tgtatttatt gacttttaca aactgggatt tatctggcag
     4021 acttgtttat ccactcatca agaaatttca tttgttcttc cgtgtgaaac caatgctctc
     4081 cgtttctcat aacagtaagt gttgcattag tttgttttgt aaattcagat atagttgcat
     4141 aagaagtcaa gttatctttc tcaccaaata aaatatttgt tggaattttc catgtagttg
     4201 gattttcttt tacataacag agatattccc atgagagtgt ttctccaaaa gttgttttaa
     4261 tttctttttt atctcgaagt tcatcttctg taacatttgc ccaatacatc atatctgcaa
     4321 tcagtttttc catattcaca ataggtgaaa taaaatatgc tttttctatt tgcttatctg
     4381 ataaggcgtt catggcgaaa taggcaccta tgctatttgc aactatttga acagatttgt
     4441 aatgtttgca aatcaaatca aaaagtgatg gaaattcttc ttttgcttcc catggatatt
     4501 gtgcagtata gttgagtcca accacatcac aatcactaaa aagaggtttg taatgtgaag
     4561 actcctctgc atttccacct ttaccatgta tataaataac tactttatcc ataatagttg
     4621 tcctcctcaa cttccgattt gtatactaaa ttgaagtata aaatcatcat ttcctgttgg
     4681 gctttctttt tattcccaat aatttaacaa aatcattaaa taagattgta tctgttggct
     4741 caaacatcaa ccattttccg tcatgatagg tttttgtttc atcataaatt ttttgcactt
     4801 cttttgaata actatttcta tctgtatcaa attttaatcg ttcatctttc cctaagataa
     4861 tcataaatcc tacacagttt tcccttgcgt acaatgcaca aagcgttttt ccgccacgac
     4921 gatatttata ctcataagtc cacgctttac cacctttgtt ccataaacat tccatgtcat
     4981 acttttcatc aattaaggca cacagtttat tccatatagc atataatgat tgcccgacta
     5041 aagcagtcat ttcttctgca ttaggtattt tatccagcat actcatttct cctttataac
     5101 ttcttattta tctttgtatt tattgacttc tacaaacaag aatttatctc atacaggcat
     5161 ttataaattt cataggcacg tccatgcgtt ctgcaacttg ttctggtgtc attccgctat
     5221 ttaagaaacg cttgcatact attttcatgt tttctccaac ttcgataata tgtttatctg
     5281 ggtcataaaa gcggactaca cgttgtcccc atgaatgttc tataataggg tggacatatt
     5341 caatatcata ttctttcagc ctatcagcaa atttatcaaa atcatcttct tcaaaataga
     5401 cttcaaaatt tttaccgcca aaagaaatat cacttgtacc gataaactct ttgtatgttt
     5461 cacttgtctg taaggctaag ccacctgtta aagttttatt tgctccaaaa tccataatta
     5521 cacgaagtcc aaaaactttt ttataaaact caactgattt atctatatca gtcactacca
     5581 acataggatt tttaaatttc attttcgcta cctccgcaag ttcaaattat ctgtttccat
     5641 acccatatta taacagtcac ttatatttgc tacaatgaaa atctagtttg ccagtttatt
     5701 tttttgttgt ccttgtggtg caaggttctg tggattgttg ttctgtggat ttcctgctgg
     5761 ctggttgcct ttgttcttca agataatatc gtcagccttg atgtaccagt ccacatcagc
     5821 ccctctaaag ttggcatttg ccaccgtatc agctacggga ttgattaact ccacttccgc
     5881 attataagga aagtccttta cgggaacatc agcggggatt gacacttgta tcatgcgttc
     5941 ctgcacacta catttcaaat cataagtacg acgcttaatt tcatcacttg tcgttccgtc
     6001 ctcattttct acacgcacct catgtcgaag tgcagagaat ttcaatactc caaaagtttt
     6061 ttcgttatct aatacaattc catttgctaa tctcataatc aaataccccc ttatttgctt
     6121 tctgctggta acatatcatc agccattaaa atgtagtttg taaatccacg tcctttgacg
     6181 ttgtaaccct ctgcggtaat gcgtgggtta atcagtttta ctttctgctc tggcttaaaa
     6241 tgcttctcgc cagcttttgc aggcaataca acgataatgt cgtcggctct ctgaatatcc
     6301 gaatacaagt tgtaacttct tgatacggga gtgtaacgcc cattgatatg ttgctggtct
     6361 actttgtttt cccctgcaaa ttccaagttt ccaaaagtct gtgccacatt cggcacgata
     6421 tattttaatt ccatattgtt ttacctcgtt tctttctatt agtttgattg ttttgtattc
     6481 tggcagttaa tgagatacca gcaagcccag acagtaaagg gtaaactgta tgctgacgca
     6541 ctcttgacga tctgggttct tgcaggtaat cggcagacaa gccagaatag aagtagtctg
     6601 cctatttaag ttacggcgga gagcgtgatt tttcttttct catgcagtta ccgccttatg
     6661 atttcttcat cttacggcgt ttgaagaagt atgtaccagc cagaccgcca gcagaaacaa
     6721 acatcataat cgcaaaggca agaatatttg tattgtcgcc cgtttttgga ctgtctgttt
     6781 tcgttggtgt atctggtgta gttggcttct ctggttcttc tggcgtaggt gtttctggta
     6841 cttccttaat agtaaccgtc tgcccgtcgt cctcaatgtc cttatgctcc gtaactttta
     6901 tcggtttgtc tggattgctt aaatcgtata attcctcaaa agttacaaac tgtttaccgc
     6961 caagagaaga agcgtcaaaa gtaaatgcaa tctttacttc catagtttca ctgtcagacg
     7021 taaatgtgta gtcattttca acttttttat cattgatgat aagctctgca ttttcttctt
     7081 ttaacatctg ccagcccaca agctgatact ttgtaccgac ttctaagccc tctaacttga
     7141 ccgtatcaac aatcgttacg tccttgccag cttcaatctc tttcttgccg tccttgtccg
     7201 tagctgtggt atggattttg atgatacgtt ctgtgataag taccgtttgt ccgtcgtcct
     7261 caatatcttt atgttccgca actttcacgg gttcttctgg attgcttaaa tcatacaatt
     7321 cttcaaaagt tacaaggttc ttaccgccaa gagcagacgc attgaatgta taagcgattt
     7381 ccactttcat agcttcatca tcagcgacaa aggtataatc actttccact cgtttatcac
     7441 ctacaagaag ctcggcattt tcttttttca acatctgcca gccttttaac tgatactttg
     7501 taccttttac aagaccgtct aatttgaccg tatcaatgat agtaacgtca ttccctgcaa
     7561 ggatagtctt ttcgccgtcc ttgctggtag cagtagtatg gatagtgatt tctttttcgt
     7621 attcatcagt cagcgtacct aaatcaacca caaccttatt tctggaaatg acaatctcaa
     7681 acggtggaat aagtttgaag cctttgttgc tttcacaacg taattcttca atgatgtagg
     7741 tatcataaat caaagcacct ttgctgtcgt ctggttcaga agtaccaaac cacacaccgt
     7801 cctcgcttgt ttttcctgca ttggtattgt gcttgtggga agcccaatca gaagcagtag
     7861 agaattgtcc gttatcatca gttacaacaa catgactttc gcccgtcgtc ttgcttgtga
     7921 tcctaaacgg aacatctgca agacgcttgt gcgaacctgc accaattttt acgccctcaa
     7981 tatcgccacg tttaatctgg ttatagatag agtgggcttc gtcagtcaaa tcaacgattt
     8041 ttccattctc tgtgatttca aaatcaatcg gctttgcacc gtcagtcaaa tatccctctg
     8101 gcggtttttg ttcagacaat ctgtattttc cgtaaggtaa taggtcagca gaagtagaag
     8161 cgataccctc aatgtcggta tggattgtct ttaacacttc atttttcttg tataacttgc
     8221 tctcaactaa gacggcattc tcatttaagg aaatgatttc aaaggcggta tttttcaaag
     8281 tcgcaccgcc ttgagctttc gtgtctttgg tttctaaatc tcgtttctgg atttttacac
     8341 cgccacgaat gactttatca gaaacatggt attgattgct tccagacaat acggcaaggt
     8401 cgccgtcctc tgtaatctgt gtcagataca tttcttttat ctgttcttcg ctaccgtctg
     8461 cctgcatata tgcaccctct aataagtaac cagacggact ttttgtttct tcgacggtta
     8521 gtgttcctaa aggaagcacg tttttaccgt cctgcatata gaagctgtct ccagatactt
     8581 tataagcgtt agctaaacgg gtaatgtaat ggatagctcc gtcgctgtct ttttcagcga
     8641 tagtctttgt tacccatgtt cttgtaggct gtgctggaag attgttcttg ttgtaatatc
     8701 cagcgtagaa gttccatgta aactctgctc cctctaaaga agcgtttccc tgcgaattgg
     8761 atttctgtgt ttccatgtca atcttgaata gctcaatcaa ggtatctgtt acttttggtg
     8821 tatcagatac ttttaaagtc gctgtttttc cagcttccac agataaagag taaactgttt
     8881 tatcaatttt aaatcctgct ggtgcagaaa gttccttgat atatactgta gtggctctta
     8941 cttcaacggc ttctgtattt ccgctggtat cagtcgtaag ggtggcaagc tgtttcgtgc
     9001 agtctttatc agaatacaca ccgtatgttg caccagcgat tgaataaatc ccgttgccgt
     9061 ctgtaatatt ggcattggtg gaagtctttt gaagtttggc atttccgaca gctaacttcg
     9121 cccagaattg ccctaactct tgaccctcgc cagaatagat atatccgcca cagtcataac
     9181 gtcctttgtt ttctttcaca aaggcttttg ctcctgcgaa tacttcatct tgtacggctt
     9241 ttgggatttc atcataggaa gctcgcacat tatcgcattg ccagtcaagc tgaacactta
     9301 atctctgcca gacaacaagc tgttttaata ggtaggcgtg tttactgctt attccgctgt
     9361 ggctgtctgt gtactgtttc acatactcaa tagataaggc aacgtcgctt atccggtcgg
     9421 cactcatgcg tgagcttgcg tctgttctgg tcttatagcc gtttttaaac gctgtgttga
     9481 tgtcaataca ataagcgtcc ttgccctcaa cggttaaatg tccctcatta aaggtagaac
     9541 tgatagaacc gtcattcatt acttgttcta cgataccgac acgctcttta ctttccgtcc
     9601 aatactgtgt attagacgca tgaaccgttg aactcggtaa agcggtaacg acagttgcaa
     9661 aggcaagaaa gcctgtacac aatcgtttta atatcttttt cataattcta aatgctcctt
     9721 tcattttggt agtaaaatag ccgtccacta ggaacggctg gaaatagaaa aggaacgctg
     9781 atattgtgcg ttccgtagtc tatgaagtat tcaatttttc ttgtttcaat actgatgtgc
     9841 tgtaccgtat ctttgcatat ctgggaaata ggacgtatca aggctcattc aatcgtagta
     9901 aattagtagt attttgatgt gtttagctcc ttgataatgc cgatagatac agcgttttag
     9961 cggataatca aatttttagt gtgtttttga tataaaaata ataatacaat acatttgtat
    10021 agtattattc taaaattcta tatctaaata aaatcacctc taaaatattt aatttttatc
    10081 tttatttgtc ataattaaat gaaatatata ttttaaagaa taataaaaga tagcttattg
    10141 tacaaaattt aaattataaa aacttagttt
//
LOCUS       DAEOSP010000142        10104 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.144, whole genome shotgun sequence.
ACCESSION   DAEOSP010000142 DAEOSP010000000
VERSION     DAEOSP010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10104
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.144"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(107..1987)
                     /locus_tag="KRH54_002680"
     CDS             complement(107..1987)
                     /locus_tag="KRH54_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBG8580321.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGSSSDSTSNVTVKKLKGADRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            complement(2191..8154)
                     /locus_tag="KRH54_002681"
     CDS             complement(2191..8154)
                     /locus_tag="KRH54_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HBG8580322.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDKQQ
                     ENSDKQEANSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSRSALE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPDGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDGLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQEISKGNSDLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGIINNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     regulatory      complement(8278..8363)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8535..9938)
                     /locus_tag="KRH54_002682"
     CDS             complement(8535..9938)
                     /locus_tag="KRH54_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBG8580323.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
BASE COUNT         2769 a         1880 c         1152 g         4303 t
ORIGIN      
        1 aacctcaata ttattagatt gttaagataa tgaaaataaa ataagaatgc atttaaatat
       61 ataactttat atctaaatac attcttattt tctatttact taatatttat ttttcaatta
      121 aactcttaat cttgtctatt acagatgagg ctattcctcc acctacttca aacaactttg
      181 atgaattttt accttttaat gacgattctt gtgatttatc taacgtattt gtagcaagta
      241 taactggtga attgttttta gctgctaatg gtccaactgc taaagcatct ataagtttat
      301 cttgatttgc aactacaact ccatctagct tagattgaga gtagaattta tctattactt
      361 tggcattcgt ctcttgtcta gtttgacctg ccaccctatt gtttgatata tctttgtttg
      421 ctatttttcc aacttgttca atagccttgt tagatatttt ttctactcca ccaataaaat
      481 atatatttga caccttattt gatttaataa attgttcagc ttcattatct acaccatctt
      541 tttgtgtaag tactattgga gttttttctt taccagccag tgatgctatt gataatgaat
      601 cagcttctcc atttcctgct cctatataca acttgcttac attattcgtc ttatctattt
      661 cttttgctat attaatagaa gtttgatatc ttgtatctcc acctattctt tgtactgata
      721 tttttgaatt tacagctttg atagcttcaa caactgaatt tggcattgag ttgtttccac
      781 ctatcacaat tacttttgat ggatttagcc ttttcaattc ttctacagtt ttttgaggaa
      841 gcttttcatt tgatgataaa agaattggtg agttttttac actagcaagt ggtgtagctg
      901 taagcccatc caccatactt ttatcttctc catttactat tacaactgta tctgattttg
      961 tccaaccact ttgagatatt ttgatagctg tctcaaatct atcagcacct tttaattttt
     1021 tgactgtgac attagatgta gaatcacttg aacttcctcc tccagagctt ccaccactag
     1081 atgagccgcc tgaattactt cctccaccat tatttccacc attgccatta tctggtggta
     1141 attcatccgg ttttgataat tcattttcaa ctatgtattc taaacttttt ggaagtgtaa
     1201 cttcaacata tttgtttatt gtagcttttc ttcccttagt tgttttttgt gctaatccaa
     1261 gttttaatat tacatttgaa ccactttctt gtgtttcttt actaacaata gaatagttta
     1321 tcctaatatt gtttaataaa tattcaagtg cttcactttt ctctacctta tacttttcag
     1381 ggtcattttt actagaacca gttccaccaa ttggttttac ttcactctca ttaataaggg
     1441 taaatattaa tccttcatca gagcttatat tttcataatc cactgttttg ttgttatatg
     1501 ttacaatttt taaagctggt ttatcatttg ttccttttcc taaaacttta tagtcagcat
     1561 ttgagttact gccagatttt tttacagata ttcttcctat attcaatcta tttccgtcta
     1621 gtaaatcttc atttgatgta acatatatat ctaatgtagt tgtattactt gatttgttgt
     1681 attctactct acaagtagca tttgcatttc cactttttat ctgagatttt atattatcag
     1741 accaagtaat cttttctttc tgctcattat ctactacttc aacctcacct tgtactttta
     1801 tactggcttg aaatgaatat actatgtttt ctactgagtc cacagatata tctatagtat
     1861 ttactttctc atacgttttt gttccttgta aaactggagc tgcatatgca catgtagcac
     1921 taccagttac agctactgcc atagttgata ctgctatagt tcttataaaa gattttcttt
     1981 tactcattaa cacccgtcct tatctatcat taactaagtg gataaattta cttatccacc
     2041 tagcttttat atttaattta taaattaccc taaatttatg gttacattta tatactttac
     2101 ttttaatatc tactatttta aaactattct agtacctaat cttattagat atacaatgtt
     2161 agtattttta cgtttttaga gttccaccta ctatttatct ctaacaatat taccctttac
     2221 tttattgctt acagttataa ccttcttatt actgatatca atagaatttc ttgtagcttt
     2281 tctagcttta ctattactat cagtaagact tattgtcttt aactcatcat aattcttagc
     2341 tcctacaggt acatacattg tatcacttac aaatctttga cctactggag tatctccttg
     2401 aaggtcattg tatctatata gttcagcctt aaccttatca cttaaagaat caaagttttc
     2461 ttttggcata tagtaaaccc atgtaccttt actgatttca cctagttcac ctttttctgg
     2521 aacttctgca aataaaagtg cttctccaga tatagtctta ttgtttttat ctataagtag
     2581 tggtaaatta aatgctggat ttccactgta ttcacctgta ataactattg aacccgctgg
     2641 aatcttacct gtttcatctt cacttgtgct agagtcatca ctttgatata cataatcttc
     2701 tgctaatttt ccaattccat tagtacgagt ttcgtctgca ttatttatta tgccaatttc
     2761 tatattatca cctggaggtc ctattatatc taattctgca acttctatat tagacttatt
     2821 atttgagatt agttttacat atctagcttg gtatgtccaa agtttaccat ctttattaaa
     2881 tgtaactcta gcttcattat cttggtcttt tcctccacca actaaatctt gtccatattc
     2941 aaaagttcct tgacttacat cttcccagtt ctttttatca ttacttactt gtatcttgta
     3001 attagttaaa ggattgtatg ttgtgctagt attctttcta aagttaaata atccttttaa
     3061 tgagaacttt ctagcttctg tctctggttt tgtatatttt agtcctacta taggtaatgt
     3121 ttctttcatg tctactatta tatatggatt ttcttctaca tttatatcag ggtctttttg
     3181 agggtctttg ttccactctg ctttacttat tatctttcct ttatatgtag tacttgcatc
     3241 attatctttt atattatcaa tttcaggatt ttgtaatggt ccatgtgagt cattttcagt
     3301 tctaacatca ccgtcagaaa cagtatttgt tatgaatgac catgctgatt tatttatttt
     3361 cccttcatct gataatttta cagtaccaac ttcaaattct tctgttggat ttaggtgata
     3421 atcatatgca actaccttat atgtaaatgc tctgttatta agaggagata tattatcagt
     3481 aaatttagtc ttgctatttc cttttgcatc cacaaatcca actgttctgt attttacctg
     3541 tgttccattt tcaccatcta aataatttcc atcacttctt attatttcgt atcctagtat
     3601 cttatcttta tccttatcta catctaggtc tatagatatt ttattagatt taactagaga
     3661 accactttgt attccatcag aaaaactagc acttacttta gtatcttttg ccatactagc
     3721 tatattttca tttaaaatct ttcttctagc ttcatcattt acatattgta tctttcttga
     3781 ttctttttcg tagttcttgc tatccattat actgtttaat tctggtgtaa tttctactcc
     3841 ccatgcctta aagtaatctc ttaaatcttt ttcaacaaca cttgatgcca ttgctactag
     3901 catttggtct tttggtaaac tattaattgg gtcactactt gaaagtgctc tgtattttct
     3961 tgaaatcttt gcatagaatg tatcattact taaatcagca tctgagttat tctttaacat
     4021 tgaacccgta aaatcatcat cataagctag atgtaattgc cataacattc caagcctttc
     4081 aaatacattg cttggtatac ctaagctatt tgaagttaca tgctcatata tcttatccat
     4141 tgttgaacct tcaagtcttg aatgtgtctt atcatcaaag gtctgagcaa gtaaagcaag
     4201 tacattgtta ctagtttcgc tatatgtcat ctttccaata tctgtaacat gtcctatctc
     4261 atgacctatt ccccatccaa ataagtttcc tcctgtagct tttccactat catctatttg
     4321 gaaaggtact ccattcataa gccctggaac tgaaccatat tctactccta catgtaatcc
     4381 agaagcatac ataaatgctc caataaacat tctttggtat tttatgttca tacgagattt
     4441 tggagccata tcatttttag ttaagttaaa ttcattatca tctacagttc cattaccatc
     4501 taaatcttgt gttttaaata ctcctctttc tgagtaaact aaatccatta tttgttccca
     4561 tgcaagcata ctattatata atctatctac ttgtgtattt aaatcagaat ttccttttga
     4621 tatctcttga tatactgctg ttgcaggaac atttaatgtt actttatcac tttcaatatc
     4681 tgtagtgttt aacacaccag ttttttctga atatccatat gaattaccaa catttgttcc
     4741 tggataatat ggttctcctt ttacagcatc tttatattta gctaattctt ctatatatgc
     4801 ttttatcttt ttcttagatt cagaaactcc cgcaggactc ttatcattta ttgttccatt
     4861 tagattaaga tgtggtatct tagtagctcc tgctacacgt acttttatat ctcctccact
     4921 tggagtatct tcagcgtatc ttatgtataa acttcctcca cattctatat ctaaactatg
     4981 aagtttgctt aattgaatta cattttttcc agtgttaaga tttatttctc cactcttata
     5041 tgcaccagat tctccatagt actgtgtata tatcaactta ggagtttttc cacttttact
     5101 tccaacatat actgtgattt catctcctgc tctagcagct actcctaatg cctgccagtc
     5161 attttgcata cctaaattat tggaatttac agtatactgt ccgtttttat ctttatattt
     5221 tcctaattta tttatttctt gatttacagt agttacatca gtaagtgtgg ctctatcatt
     5281 atataagtct tgagctaatt ttaattctct ctcaataact gtcttatatg gatgatattc
     5341 tccactagct ttatctggag tatttaatct atcttttaat tcagttattt tttcttgagt
     5401 tacatcttct tttaattcaa cttgtaggcc atccataaat agatttctta catcatcttc
     5461 tatactgtca tactcataaa acttaagttc tgatatatta gacttatatg aataggatga
     5521 acaaacactt acttgtatct tggtagcagt tattggttct tttaatctaa ccattacata
     5581 cttattatta tttacagggt ctgtctttgt ttcaaaagga acatccccca gattgtgtaa
     5641 ttgaccattt tcatcccaat atcgtacctc aaagtcattt attgtcttat cagaaacatc
     5701 aaaacgaggt gtagccataa aagtatccat agtataagat ttatcaaaag ttattattgg
     5761 tcctctattc gttccctcag ctgcattata tgttgggaat gtccaatgtg ttgtaaagtc
     5821 cccatctact acattgaatt tatcaaatgg atttttatca ctatattcac ttttgtcata
     5881 tccatctgga taaacaacat ctacaatgtg atttgttggt attccaaatc caccattttc
     5941 tttaggagta ttaattaatc tatagttagg tgttgttggt gcatccatgc tttcagttgt
     6001 acctatatat gttttagaca ttccactagt tcccaagtgg ttagtagctg tcattctgac
     6061 ttcatatgtt gtattatcct taagaccttc tatctttgaa cttgtcccac gaataagctt
     6121 atcattgtat tttatccaag acccattaga gcctttttct ttatagtaca agtcaaaatc
     6181 tttagcttgt ttatgatttt tccagcttac atcaagagat ttaaatttac ctttaacatt
     6241 tataccttct ggtggttctg gttttgtgtc tggaacaact tgaaattcta atatactatc
     6301 tgggctaaat tgtataggtt tatagtttga atctacattt tctggtactc cgtctaagtt
     6361 ttctggtgtt gcatctttat aaccactaga ccaatctcca tttaaagatt gtatgctaac
     6421 tctatatagt ccatatgctt ctatatctct aacttctatt gtatttttac tagtttgtaa
     6481 ttttttagta gataatactt ttcccttact atcaattttg tctagtataa gttcgtatgc
     6541 tgatacattt acttcattat tccaataaaa agatactttt tcatttccaa cagcagtgct
     6601 agtcgatatt ttatttattt taggtatgtt catctctggc tttggtattt ctttatatac
     6661 attgtttaaa aattcaactt ttgatatttc tgcaagattt ctatcgtttg ctgttgtagc
     6721 tgttattttt atagttatct tttttactgc tacttgttta ccaagattta ttactatatc
     6781 actattttga acatctgcat atgtttctaa tgctgaccta ctatatgtag atgttgtaga
     6841 gttatcatct ttactgatat taccctcttc ttttggagta tcttcatctg aattaggttt
     6901 atccattgat gaattttctt tatcattatt tttatcttga ttggtattag agtttgcttc
     6961 ttgtttatcg ctattttcct gttgtttatc attattttca tctttattat ttgagttatt
     7021 atcagtctgg tttatatttt catctttatt atttacttct tcttgattta cttcttcttg
     7081 ttcgttattt acttcctctt gttgctcctc atcttttaag tttttcatat attctttgtt
     7141 ttcataagga atctgttgaa cattaccatc tttgtcagta acctcaacta ttccttttgc
     7201 tggagcagta ccttctggag cttttatatt gatgacttcc atattaagtg cttctgataa
     7261 agctatatcc attactgctg gtttttcctc agatatttca tcattttttg aagatattgt
     7321 aactccatta ttttctccac taaataaatt ttctacatta ccattaatat ctacatcact
     7381 atttcctttt aattcaacct tacttgtatc tactatagga ttggtagata ctatactttc
     7441 taggcttttt gcatcttcac tttgtcctaa atttttctgt acataatcta agtcaacaat
     7501 atctaccttt ccatctctat ttaaatcaaa ttctctttta tttgaatcta tattctctaa
     7561 aaccttatta taatcactct tatctacttt accgtctcca tttacatcac ccattaagaa
     7621 caattcataa tttaaaggtt tatttttatt atctacactt gctctattgc ttaataagac
     7681 tctttgtgaa taacttgata tatcaatgtc tttaatttct ttgttcttaa atccatctcc
     7741 agagacttct acattgtatt ttccaacagg taaattatta aatacaatat tataatatga
     7801 tatattctca tctttatttt ttatttcact tctacttgca gataattttt ttatagtata
     7861 ttctatatta tctgtcccaa catttactgt tcctgaatct ttttctccaa gctttattat
     7921 tccactattg ttactatcat cttttaatcg taactgcata ttagtcatac tttctgactc
     7981 gctatttttt attggcagtg aaaaatttaa atctacttca agactccccc ttaacttttc
     8041 tgtaactgat ttttcaactg cccttagctt cccctctaat ataggttctg caaaaacgtt
     8101 tagtggtgca ctacacatag atactgctag tacacaagat attgcctttt tcatgaaata
     8161 acaatcctcc cttaataaat atctaatttt tcatgaattt aaatactata agtttcagac
     8221 aataaaaaaa gacactcaaa gagaatctta attataaaat aaaaagactt tactcttttt
     8281 agcaactggc tgatatggct ttaaaagctt taatccctat agttttgcgt cattagattt
     8341 ccctaatttt gccatttaat tttcactact ttcttaccca ttttatacta tatttaaact
     8401 ttttaatata atttttttag attaaattat aaaaaaataa catttatatt aatttattaa
     8461 tataaatgct attttccatt ataatttaat tatttatata gcttccttac aagcataatt
     8521 ttgtgtatat tttcttattc tttatatctt ccataaaatt catgtgtagc atatagattc
     8581 ttatctcctg ttatagctat accaaatccc attatttcat accctttatg caacatatta
     8641 gttccttctt ctggatttgt cttccactca ttaaaaataa agtttcctat atcatctgca
     8701 tctctatttg tatatacagg tttatctgga attttatatt taattatatt ttcaccttga
     8761 actgctatat atccaggttt aatctcactc caatctaagt atttcaaaaa agtagaatat
     8821 gaattttttc catttatcgt atgagataaa gtcttcttat ttgccattaa attagaccaa
     8881 tcatttgcta gaccctctaa tctgcttgct acagataacg ctcttactcc attctctttt
     8941 ctatactgct ttgtaagagc atatacttta ccctttattc tctctttaaa tgctctatca
     9001 tttggattgt aatataaacc ttgttgattt ggtgaagctt gatttataca ttgcttgata
     9061 acattctgat ttatgcctcc tagtacagtc aatttgtctg caccacttaa aatactcttg
     9121 ttactgccat tttcaactaa aactacagga gcattttttg ctattgtaga ggcagccaaa
     9181 gcgtctgtta attgtaagcc tttacttaga taaaattctt tactattttt ataaaactct
     9241 tgtataacta tcttattagt ttcaaatcta tctgtgccac ccaatctaat agaatttgtg
     9301 ctcttaacta attcgtcgct tattatttca ctagaaccaa gcgcataact ctttaagcct
     9361 gtcgtgtcaa atcccacact atttccatct gtaagtatta taggtactcc atctctagca
     9421 gctactggtg atgcacttat cgcatctgct tctcctgtaa atccattggc tataattact
     9481 ttatctattg gcttaatttc tttgattttt tcagctattt ttatacttgt atcaaatcta
     9541 ttttcaccaa atattctttg tacaaccttc tttttggata gtaactgatt ctcaacattt
     9601 ttacttattg ttgattctga acctattata tacacagtct ttgctttgtt taatctttcc
     9661 atagttgtgt ttggaatctg gttttgtttt gtcatcaaaa taggcgcatt taaggctcca
     9721 gctaaaccac ttgcacttag accatctgct aagctattat ctgagttgat tagtattgct
     9781 gtattgtagt tttgcttatc tgctattttt gctgctgtct catatctgtc attccctttt
     9841 atttgctcaa ctgatgtcaa cgcatttacc ataggagtat ttaccattac taaaaatagt
     9901 gtaagtccta tagctaatat ctttttacta ttcttcatat tattttctac ccccaatgta
     9961 cttttcatat tttttattag tactcatact tatttttaat caaaagttac aattttgtta
    10021 ccatatttta tatatgatac catagttatc catacaactc aaacaatata cctaaagttt
    10081 ttattctcta taaattcaaa acat
//
LOCUS       DAEOSP010000143        10090 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.145, whole genome shotgun sequence.
ACCESSION   DAEOSP010000143 DAEOSP010000000
VERSION     DAEOSP010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.145"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            69..1109
                     /locus_tag="KRH54_002683"
     CDS             69..1109
                     /locus_tag="KRH54_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBG8580324.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            1248..1700
                     /gene="tsaE"
                     /locus_tag="KRH54_002684"
     CDS             1248..1700
                     /gene="tsaE"
                     /locus_tag="KRH54_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBG8580325.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            1700..2416
                     /gene="tsaB"
                     /locus_tag="KRH54_002685"
     CDS             1700..2416
                     /gene="tsaB"
                     /locus_tag="KRH54_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBG8580326.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            2406..2882
                     /gene="rimI"
                     /locus_tag="KRH54_002686"
     CDS             2406..2882
                     /gene="rimI"
                     /locus_tag="KRH54_002686"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBG8580327.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            2888..3904
                     /gene="tsaD"
                     /locus_tag="KRH54_002687"
     CDS             2888..3904
                     /gene="tsaD"
                     /locus_tag="KRH54_002687"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBG8580328.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            4201..6909
                     /gene="hpdB"
                     /locus_tag="KRH54_002688"
     CDS             4201..6909
                     /gene="hpdB"
                     /locus_tag="KRH54_002688"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBG8580329.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVQILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            6913..7170
                     /gene="hpdC"
                     /locus_tag="KRH54_002689"
     CDS             6913..7170
                     /gene="hpdC"
                     /locus_tag="KRH54_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBG8580330.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            7163..8113
                     /gene="hpdA"
                     /locus_tag="KRH54_002690"
     CDS             7163..8113
                     /gene="hpdA"
                     /locus_tag="KRH54_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBG8580331.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSDENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            8428..9390
                     /locus_tag="KRH54_002691"
     CDS             8428..9390
                     /locus_tag="KRH54_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG8580332.1"
                     /translation="MNKSWSNLIIKNKDRKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFAWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(9489..>10090)
                     /locus_tag="KRH54_002692"
     CDS             complement(9489..>10090)
                     /locus_tag="KRH54_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG8580333.1"
                     /translation="IIIYSSNKKNLYPVKNIKLGSLLIIYFMISVIVPTSFILILQGA
                     QPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKLGVIILGMCWSL
                     WHLPLIFTLYTPEAPILGIILRSIHIVGISIFLGYLYIKTKNIWFCAIIHVLNNSAFL
                     ITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
BASE COUNT         3955 a         1052 c         1868 g         3215 t
ORIGIN      
        1 aaaactataa agattaataa tcaaactttt aataataact aatttaataa tgataggggt
       61 gtatattcat gcatttatct aaaaaaataa aaaaattatt tttagatgat aaagggtatt
      121 attttaatag taattggaaa gatattcata ttggtattta tgatacaaaa ttaggagatt
      181 ctttaaagta tatcactaat gaatatattg aaaatatgga aagggtaaaa tacgattatc
      241 gtgaattaaa aaatatagaa aatcaaactg atggtatgtg tattttagca ataaggcaga
      301 attacaaggc attagagtat gtaaaaaatc aaaccgaaga attatgttta gaagcaataa
      361 agtataatta taaagcactt gaatatgtaa aagaacaaac agaatacttg tgtcttgaag
      421 ctataaaaaa agattgtaat gcacttaagt atgtacgaaa taaaacagaa gggcttatta
      481 taaaagcaat aagtcattct tccaatatag atgttgtaag tatactaaag gctttagaga
      541 ctcaaacaag aaggatatgt ttagaagcaa ttaaaaaaga tggcaggtgt cttgcatacg
      601 ttagagaaca gagtgaagaa ttatgtatag aggccattaa gcaaaattat aaagcattaa
      661 aatatgtaaa aaatcaaacg gaaaaaatgt gtatagaatc cgtaagacaa aatggtatgg
      721 cattacaata tgtaaataaa caaactgata aaatatgtat agaagctgtc aaacaagatg
      781 gaagaagtct acaatttgtc aacaataaga cagaagaaat atgtataaat gctataagat
      841 atttaaataa aaaatataat attaaagatg tactaagtta catagataag tatacagaag
      901 atatatgtat agagattgta agacaaaatg gaaaaatgct tatgtatata aaaaatcaaa
      961 cagaaaaaat gtgtattgaa gcagtaaaag agaactataa gtctcttaag tatgtaaaag
     1021 agcaatctga gagaatatgt aaagaagcat tgaagcaaaa ccataaagca aaagagtatg
     1081 taaaaattgc tattgatgat tgtatataag gtgtagatac agccatcaat agtgaaagat
     1141 aaaaaaagat aaagacaagt actttataat aatctaggct tatactataa ttaattaagc
     1201 agtgatataa attcacatga cgattatatc aaaatggaga tgaattgatg gcaaaaatat
     1261 atttagaaaa tgaaaataaa actagagaaa taggatataa attaggtaaa cttttaaaag
     1321 aaggcagtgt tatctgttta gtaggagatt taggagctgg taaaactaca atgacacaaa
     1381 gcttggcaga ttcattaggg atagaagatt atataacaag tcctactttt actataataa
     1441 atgaatatga aggaaaaata cctttgtatc attttgatgt ttatagaata ggtagtagtg
     1501 atgaaatgta tgatattggt tatgatgaat atgtaaattc aaatggaatc tgtataatag
     1561 aatgggctaa tctaatagaa gatatacttc caaaagaata tttgaatata gaattgagat
     1621 ataaagatga aggaagagaa atgatattaa ctccaaaagg agagttttat aaagagatag
     1681 ttgaggagtt gattaaataa tgaaaatact aggtatggat acgtcttcta tggcagcaag
     1741 tgtagcagtt gttgaagatg ataacttaat atgtgagttt acagtaaaca acaaaaaaac
     1801 tcattcacaa aagcttatgc caatgataga aaatatgttg agtatgagtg atttaagtat
     1861 aaaagatatg gatttattag ctgtatgtat aggtccaggt tcgtttacag gacttagaat
     1921 aggtatggct acagtaaaag ctatggccca tgtaaataat ataccaataa tagcagtaaa
     1981 ttctttggaa tctctagcaa ataacataaa tttctgtgat agaaagatat gttgtatatt
     2041 agatgcgcag aaaaatcaag tttattcagc taaatacaag tttgaaaatg gagagatgat
     2101 agaattagat ggtgttgatg ttgtagagtt tgatgcttta gtagatgaaa ttgtatcaac
     2161 taatgaagaa tttataatag ttggagaagc tgtatacaaa tataaagaca agttagagaa
     2221 tataaaaaat ataaaaatac cttcaccagc taataatgta tctaacgcag gaagcttatg
     2281 ctctttagca ttaaataaat ataataaaaa tatagatgta catacttgtt atacgataaa
     2341 tcctatgtat atcagaaaat ctcaagctga agtacaatat gatgaaaaaa tgaagaggct
     2401 aaataatgga agataataca aaactaatag tagatgatat aaaaatagaa gaaatgacta
     2461 cagaagatat agatgaagtt tttgaagtag aaaaaaattg ttttgaagat tattggtcaa
     2521 aagagtcttt tagaaaagag ttgtctaatg aagtagcaag gtatatagta gctaagttaa
     2581 atggaaaaat tgtcggatat gtgggaattt ggcttatact tgatgaaggg catataaata
     2641 atgtggctgt tcatagtgac tatagaggga aaaaaatagg ggacaagttg ataaaaggca
     2701 tagttgattt atgtaaagat aataatattg catctatgac attagaagtt agagcatcaa
     2761 ataaaatagc acaaaattta tatagaaaat atggatttaa aatgggtgga ataagaaaag
     2821 aatactacag tgataataaa gaagatgcaa ttataatgtg gaaccaatta aaagaggtgt
     2881 agagtttatg agtgatataa taacattagc tatagagagt agttgtgacg aaacagctgc
     2941 atcagtttta aaaaatggta gagaagtact ttcaaacata atctctactc aaatcgagac
     3001 acataaaaaa tttggtggtg tagttccaga agtggcctca agaaaacatg tggaaaatat
     3061 tgatatagtt gttcaagaag ctttagacaa agcaaatata ggttttaatg atatagacca
     3121 tatcgcagtt acatatggac ctgggctagt aggtgcttta ttggttggtc tatcttatgc
     3181 taaagcactt gcatatactt tgaatattcc acttgtagga gtaaatcata tagaagggca
     3241 tctaagtgca aactatatag agcataagga tttaaaacct ccttttataa cattaatagt
     3301 atctggaggt catactcact tggttgaagt aaaagattat ggcaagtatg aaattttagg
     3361 aaaaacaaga gatgatgcat ctggagaagc atttgataag atttcaagag ctatgaattt
     3421 aggctatcca ggaggtccta ttattgataa tctggccaag aatggaaata aacatgctat
     3481 agaatttcca agagcctatt tagaagaaga tagctatgat tttagtttta gtggattaaa
     3541 atcctctgta ttaaattatc taaatggaaa aagaatgaaa aatgaagaaa tagtagttga
     3601 agatgtagca gcatctttcc aagaggctgt agtagaggtt ttatctacta aagcactaaa
     3661 agcagtaaaa gacaaaggat acaatatcat aactctttct ggtggggttg cttctaattc
     3721 ggggcttaga gctaaaataa cagagcttgc taaagacaat ggtattactg ttaagtatcc
     3781 tcctctaatt ttatgcacag ataatgcagc tatgataggg tgtgcaggat attataattt
     3841 tataaatggg aaaacgcatg atatgagttt aaatgcagtt ccaaatttaa agataaatca
     3901 gtagagttag atgaatttgt aaatataaaa gtattgtttc aaaaaagtaa gtagaagtga
     3961 aattaaatat aataaaaata aatatagtat atattaaaac ttgaagtaaa actatttata
     4021 atctttatat tccaatgaat tgcaacaaat tttaggatat acagattgtc tttggcttag
     4081 atttgcttca agtttaattt tttctataga aagattattt taaaaagtgg gtaatatacc
     4141 cttttttttc attcttaaaa agttttatac taattataga aagattaagg aggggaagaa
     4201 atgagtcaaa gtaaagaaga caaaataaga agtattttgg aagcaaaaaa tattaaatca
     4261 aattttcaaa ataaagaaaa tttaagtgaa tttaatgaaa aaaaagcaag taaaagagca
     4321 gaagacttat tagatgtata ttataataca ttatctactg ctgatatgga atttccatat
     4381 tggtataata gagaatatag aaaatcagat ggtgatatac cagtagttag aagagctaaa
     4441 gctcttaaag ctgctttttc acatatgaca ccaaatataa tacctggtga gaaaatagta
     4501 atgcaaaaga cacgccatta tagaggttct tttccaatgc catgggtaag tgaaagtttc
     4561 tttgttgctc aaggagaaca aatgagagaa gaggcaaaga aattagccag taacacagct
     4621 gatgaactta caaaatttgg gtctggtgga ggaaatgtta ctgaaagttt tggaaatgtc
     4681 gtttcaatag ctggtaaatt cggaatgaga aaagaagaag tacctgtact tgtaaaaatg
     4741 gcaaaggaat gggttggaaa gtctgttgaa gatttaggtt ttcactatga aaaaatgatg
     4801 ccagattatg atttaaaaga aaacctaatg agtacactta tatgcatgtt tgattcaggg
     4861 tatacacttc ctcaaggtag agaagttata aattattttt atcctttaaa ttatggatta
     4921 gatggaatta tagaaatggc aaaagaatgt aaaaaggcag ttgcaggaaa tgcatctgga
     4981 gatgggctta ttggtatgga ccgtctatat ttttatgagg ctgttattca agttattgaa
     5041 ggattacaaa cttggatttt aaattatgca aaacatgcaa aatatttaga aagtatagag
     5101 acagatttag aagctaaaaa agaatactca gatttagtgc agatattaga acatattgca
     5161 cataaacaac ctcgtacatt tagggaagct cttcagttga cttatacaat tcatatagca
     5221 tcagtaaatg aggatgctat atctggtatg tctataggac gttttggtca aattttatat
     5281 ccttggtatg aacaagatat agaaaaagga ctaattacaa aagaagaggt tatcgaatta
     5341 ttagagcttt atagaataaa aatcacttgt attgactgtt ttgcttcagc gggcgtaaat
     5401 ggaggagtac tatcaggcaa tacatttaat actttatcta taggtggatt aaaagaagat
     5461 ggttctacag gagctaatga actagaagag ttattattag aagcaagtat gagatgtaga
     5521 actcctcaac ctagtttaac tatgctatat gatgaaaagc ttccagaaga tttcttaatg
     5581 aaagctgctg aatgtacaaa gcttggttct ggttatccag catgggtaaa caactcaaat
     5641 ggaacaacat ttatgatgaa acaatttgct gatgaaggta tgactgttga agaagcaaga
     5701 gcatttgcat taggtggatg tttggaaacg tctccagggt gttggaaaca gttaacttta
     5761 aacggaaaaa catactctat tgcaggtgga gcaggtcaat ctgctggctc tggagttcac
     5821 tttatagcta atccaaaaat tttagaactt gttcttatga atggtaaaga ctatcgtatg
     5881 aatatacaag tatttgagcc tcataataag ccattagata cttatgaaga agtcatagaa
     5941 gtatttaaag attattataa acaagcaata aatgttttag aaagagcaaa taacattgag
     6001 ttagatatat ggagaaaatt tgatacttct ataataaatt cactactaaa gccagactgt
     6061 ttagacaaag gtcaacatat aggaaatatg ggatatcgtt ataatgcaac attaaacgta
     6121 gaaacttgtg gtactgttac tatggtcaat tcatttgcag ctcttaagaa acttgtatat
     6181 gatgacaaag catttacaat agaggaaatg aaggatgcca tattaaataa ttttggattt
     6241 aaagatgcat tagaagtagg aaattattct atggcggacc aagtaaaagt agataagact
     6301 ggtaaatatg atgctattta taaggcctgt cttgatgcac ctaaatatgg aaataatgat
     6361 ttgtatgcag ataatattct taaaaactat gaggtatggt tatcaaaagt atgtgaagaa
     6421 gcacaatctt tatatgctaa gaagatgtac ccttgccaaa tatctgtttc tacacatggg
     6481 ccacagggag ctgctacctt ggctactcca gatggtagac taagtggaac aacatattca
     6541 gatggttcag tctctgcata tgcaggaaca gataaaaatg gagtatatgc attatttgaa
     6601 tctgcaacta tttgggacca agcagtagtt caaaattctc agatgaattt aaaacttcat
     6661 ccaactacta taaaaggaca acaaggaact aaaaaattat tagatttaac aagaagttat
     6721 ttaagaaaag gtgggttcca tattcaatat aatgttgtag attcagaaac attaaaagat
     6781 gcacaaaaaa atcctgacaa ttatcgtcaa ttaatggtac gtgttgctgg gtttactcaa
     6841 tattggtgtg aattaggtaa accaatacaa gatgaagtaa ttgctagaac agagtatgaa
     6901 ggggtgtaaa atatgagaaa gcatagtgat tgtatgaatt tttgtgcagt agatgcaacc
     6961 aaaggaattt gtagattatc aaaacaaatg attaatttag atgatgcagc atgtccagag
     7021 ataaaagtaa tgccaaaatg taaaaattgt aaaaattttg ttgaagctaa tgatgaaggt
     7081 ataggcaaat gtgttggcct agagaaagaa gactgggtat attcaacatt gaatgcaatt
     7141 acttgtgaag gacatgtgtt taatgagtag tcaaaagcaa ttagaaggca tgatttttga
     7201 tgtacaaagc ttttcagttc atgatggtcc aggttgtaga acaactgtat ttttaaatgg
     7261 atgtccactg agttgtaaat ggtgtgcaaa tccagaaagt tggactgtaa gaccacatat
     7321 gatgtttagt gaattatctt gtcaatatga aaatggatgt actgtatgtc atggtaagtg
     7381 taaaaatggt gcattgagct ttaatcttga taataagcca gttatagatt ggaacatctg
     7441 taaggactgt gaaagttttg aatgcgtcaa ctcatgttat tataatgcgt ttaaattatg
     7501 tgcaaaacct tacactgtag atgaacttgt gcaggtaatt aaacgtgatt ctaacaattg
     7561 gagaagtaat ggaggagtaa catttagtgg aggtgagcca ctacttcagc atgaattttt
     7621 acatgaagta ttattaaagt gtcatgaggt aaatatacac actgccattg aaacgagtgc
     7681 atgtgtatca aatgaagttt ttaataaaat atttaacgat attgattttg catttattga
     7741 tataaagcat atggatagag aaaaacacaa agaacaaaca ggtgtgtata atgatttaat
     7801 attagaaaat atatcaaacc ttgcaaattc tgattggaat ggaagattag tacttagagt
     7861 accagttatc tctgggttta atgatagtga tgaaaatata agtgatatta tatcttttat
     7921 gcacaaaaat aatttggtag aaattaatct attaccattt cacagattag gagaatcaaa
     7981 atggattcaa ttaggcaagg aatatgagta ttctgataag ggtgatgttg atgaaggtca
     8041 tctagaagaa ttacaagata tatttttgga taatggtata gcttgttatg ttggccatga
     8101 gacagctttc taaaattaaa tacaagtttt aattaaaaaa ggatgttaca ctaaaagcta
     8161 gagttacatc cttttttatg tggtttttat gtagaaataa ttaagtaaag ttttatatgt
     8221 tttatatatt gtttatattg ctttatgtcc ataaatattg ctttaaatat actttatata
     8281 ttttttatag tttaaattac agttagagaa tatattacgt ttacttcatt gaagcagaag
     8341 ttttgtggta aaatactact atgaaaaaat ttaatagaat atattttaga aacaactaaa
     8401 aataatagtt aggaggaaga catctatatg aacaaatcat ggagcaactt gattattaag
     8461 aataaagaca gaaaagtacg ttcagggtgg attattatag ctgttatggc taccttttac
     8521 atacttcagt attatttaag catgatactt gtggaaataa taagaaaaat cctggttgct
     8581 acaggtgaca ttaatttaaa aacaaactat ttttcagaat atgctaattg gttaaatgat
     8641 gttgggttac agattgtatt tcaaattcta acagattcac ttatgattat tattcctata
     8701 attgtatgga gatttattat gaaaaatcct attagtgaaa tgggattaca ttcttataga
     8761 attaagaaaa aagaaggata tgtaggaatg ttattaggaa tatttaattg tacagtaatc
     8821 tttattataa caataacaat tggtggtggt catgttactt catgggtacc aaaaatttca
     8881 cctttaacag tttcttggat actggtcttt attttagttg cttttgcaga agaaatttta
     8941 aatcgtggtt tttttatggc agttttacga agatgtaaaa atatttattt tataatgatt
     9001 gttccatctg ttatttttgg gttgattcat atatggaatc ctgatgttac ttttttgtct
     9061 gtaattaata ttataattat tgggattctt ttttcatata tgtttataaa atcgtcgaat
     9121 atatggatgt gtataggtta tcattttgca tggaatgtat ttcaggggat tatatatggt
     9181 atgcctgtaa gtggacttca agttccaggg attataacta cacaaatcac tcatggtaat
     9241 ctattaaatg gaggaatgtt tggtattgaa ggtggaattt tgactacttt tgttaatcta
     9301 cttagcttta tatttgtatg gtattactat agagattcta agtatgattt tgttagtgat
     9361 aaagtaaatg tgaccaatat caagaattag ttgattataa taaattttaa taatatcttc
     9421 taagcttaaa gtattctgta ggattgttgg aaatttgcaa aaatataaaa ataacttgga
     9481 tattctattt actccaagtt atttttatat tcttttgtaa atagaaatgg aacataaaaa
     9541 attaaaacaa ccatagatac tattagtata tcatggtaag tgatagttga ttctttagaa
     9601 tttgtgataa gaaaagcact attatttaaa acatgaatta tagcacaaaa ccagatattc
     9661 ttagttttta tataaagata tcctaaaaat attgaaattc caactatatg tattgaacgt
     9721 aatattattc ctaatattgg cgcttcaggt gtatataatg taaatattag tggtaagtgc
     9781 cacaagctcc aacacatacc taatattata actcctaact ttttaccaaa tttatcaaaa
     9841 aaaatattct gtaaaaaacc tctccaggca tattcttcac taaaaaaaat ataaatagta
     9901 gttaatgatg ttattggagt aaatataaat tttatgatac cactataatt aggttgagcc
     9961 ccttgtagta ttagtataaa tgatgttgga acaattacag aaatcataaa gtaaataatt
    10021 aataaagaac ctagtttaat attttttaca ggatataaat tttttttatt tgatgaatat
    10081 attataattg
//
LOCUS       DAEOSP010000144        10047 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.146, whole genome shotgun sequence.
ACCESSION   DAEOSP010000144 DAEOSP010000000
VERSION     DAEOSP010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.146"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            50..1117
                     /locus_tag="KRH54_002693"
     CDS             50..1117
                     /locus_tag="KRH54_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBG8580334.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            1287..3188
                     /gene="cooS"
                     /locus_tag="KRH54_002694"
     CDS             1287..3188
                     /gene="cooS"
                     /locus_tag="KRH54_002694"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBG8580335.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAECIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            3181..3654
                     /locus_tag="KRH54_002695"
     CDS             3181..3654
                     /locus_tag="KRH54_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG8580336.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSRHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            3629..4855
                     /locus_tag="KRH54_002696"
     CDS             3629..4855
                     /locus_tag="KRH54_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG8580337.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(4994..6181)
                     /locus_tag="KRH54_002697"
     CDS             complement(4994..6181)
                     /locus_tag="KRH54_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG8580338.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            6454..7278
                     /locus_tag="KRH54_002698"
     CDS             6454..7278
                     /locus_tag="KRH54_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBG8580339.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            7407..8672
                     /gene="gluD"
                     /locus_tag="KRH54_002699"
     CDS             7407..8672
                     /gene="gluD"
                     /locus_tag="KRH54_002699"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBG8580340.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            8885..9475
                     /locus_tag="KRH54_002700"
     CDS             8885..9475
                     /locus_tag="KRH54_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBG8580341.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFICIMEKYMDLDCEFYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRGTNFSNKFVAGLNKQSDIIKRVMNMFSEKHKNGI
                     LMALIISLFSSITFFKFDSTSIIEENDFLERLRYTFEGRQEDMFVTIDFTYADTPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
     gene            9805..>10047
                     /locus_tag="KRH54_002701"
     CDS             9805..>10047
                     /locus_tag="KRH54_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBG8580342.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFN"
BASE COUNT         3924 a         1077 c         1799 g         3247 t
ORIGIN      
        1 aaaaaagtat attttattta aaaatttatt aatacaaagg agatttacta tgaaaaataa
       61 attagtttac atatgtatta tgagtctatt gctatttgta gtaggatgtt ctaataatcc
      121 aaataaggaa agtaccatta aaaaagactc taagaataac caaagccaag ttaaagaaga
      181 aaaaagagaa cctgtgaaaa aagatataac tataagtttc gctggtgatg taactatggg
      241 gaactataaa ggttcatcat attatggcag ttttgaccat gaatttaaaa gacaaggaga
      301 agattacaat tattttctta aaaatgtaaa acctgtattt gaaaaagatg acttaacagt
      361 ggttaattta gaaggtccac ttactacagc gtcaaatgca aaagaaaaaa aatttgcatt
      421 taaaggaaag cctggatatg taaatatttt aaaatctgga agtgtagagg cagtaagtat
      481 tgcaaataat cattcagaag attattttga agaaggaatg aaagatacac agtttatatt
      541 ggacgagaat aaaataaatt attttggatt agggaaagat gctgttgtag atgtcaaagg
      601 aattaaagtg ggtctattag ggtataatgg attatcaact gaatataatg aaaaaaattt
      661 aaaacaaatg gaagatgata ttaagtcctt aaaaaagaaa tcggatgttg ttgtagtgta
      721 ctttcattgg gggatagaat tagattatta tccagataaa aaacaaaaag attttgctca
      781 ttatgctata gacaagggag cagatttagt tatggggtct cacccacatg tgattcaagg
      841 aatagaaaag tataaaaata agtatatagc ttatagtcta gggaattttt gctttggagg
      901 aaataagaat ccttctgata cagatagtta tatatatcaa caaaccttta ctttttcaga
      961 taataaatta acttcaatta aagagccaaa tataatacca acttcaataa catctagtaa
     1021 ctccagaaat aattatcaac caaaaatatt ggatggaagt gagaaggata gagttttaaa
     1081 taaacttgaa aaaataagca aagatttaaa taaataaaat gtaaaaaaaa ataaatatat
     1141 atatttattt taataattta tattttgttt agggtatggt aaatcgtggt attaaataag
     1201 tagattttaa tagttaaatt atagtcaatt ataataaagc tatttaatct acttttttaa
     1261 tataaaaata taagggggaa tttgtaatgg cttgtaacac ttgtaaaatg tgcgaaagtg
     1321 cagacaagaa attagaaagt tttgtagctt caaaggatgt tgaaacagca tttcatagaa
     1381 cagaagatca gaaagttaag tgtggttttg gacttcaggg ggtttgttgt agactctgtt
     1441 caaatggacc atgtagagta actcctaaat caccaagagg tatatgtgga gcagatgcag
     1501 acactatagt agcaagaaac tttttaagag cagtagcatc aggagcagct tgttatttac
     1561 atgtggtaga aaatacagct aagaatttaa aaaatgttgg aataactaaa ggtgttgtta
     1621 aaggagaaaa aacattaaat caattagcag agatgtttgg aatagaaagt gatgaaaagt
     1681 atgataaatg tataaaggtt gcagacaaag ttatcaacga tttgtataaa tcaagagatg
     1741 ataaaatgga attagtagaa aaaatagctt atgcacctag agtgaaaaag tggaaagagt
     1801 taggtataat gcctggtggt gcaaagtcag aagtttttga tgcaatagta aaatcatcaa
     1861 caaatttaaa cagtgaccca gtagacatgc tagttaattg tttgaactta ggtatatcaa
     1921 caggattata tggtcttaca ctaactaatc ttttaaatga tgttatgttg ggagaaccag
     1981 ttataagaat ggctcctgta ggatttaatg taatagaccc agactacata aatataatga
     2041 taacaggtca tcaacattca acatttgcaa atttccaaga tagattaaaa gatgaagatg
     2101 ttataaaatt agctcaatct gttggcgcaa aaggatttaa gttagtagga tgtacatgtg
     2161 ttggtcaaga tttacaatta agaggagaac attatcaaga agtatttgct ggacatgcag
     2221 gtaacaattt tacaagtgag gctgttttat caactggagc aatagatata gttctttcag
     2281 aatttaactg tactatacca gggcttgaac caatagcaga taaatataaa gtaaaaatgg
     2341 tttgtttaga tgatgttgct aaaaaagcga atgcagaatg cataggttta gacagaagta
     2401 aattagatga acttagtaac actttaatag aaaaagcatt ggagtcttat aaggagagaa
     2461 gaggaagtat tgaaatagat ataccaaaag accatggatt tgaacaatct ttgactggtg
     2521 taagtgaaaa gaacttgaaa gcgtttttag gagattcatg gaaacctctt attaatttaa
     2581 ttgcagaagg aaaaattaaa ggtgttgcag ctgttgtagg atgttcaaat atgacagcag
     2641 gaggacatga tgtaaatact gttgaactta ctaaagaatt gattaaaaaa gatataatag
     2701 tactatctgc aggatgttct actggaggat tagaaaatgt tggtcttatg tcacctggag
     2761 cagaagaatt ggcaggagaa aacttaaaag aagtatgtaa gactttagga ataccaccag
     2821 tattaaactt tggaccatgt ctagctatag gaagactaga aatagtagca acagaattag
     2881 cagctgaact tggaattgac ctaccacaat taccattagt gctttcagct ccacaatggt
     2941 tggaagaaca agcattagct gatggagctt ttggtcttgc ccttggctta cctcttcatt
     3001 tagctttacc tccatttata acaggaggaa aattggtaac agaagtatta acagaaaaat
     3061 taaaagactt aacaggagga catgttatag tgaatccaga ccctaaatct tcagctaatc
     3121 aattagaaga aataataata gatagaagaa aaaatttagg gttaaaggat gtggaatgca
     3181 atgcatagaa tttttataaa taaagattta tgtacaggat gtaagagctg tgtactagca
     3241 tgtatgctaa aacacaataa agattatgac atgtacactc ttgatttaga aaatatagat
     3301 aatgatagta gagggcatat agaattagat agtagacata ataatccagt tccaattctt
     3361 tgtagacact gtgatgaacc tgaatgtgta ttagcatgta tgagtggtgc aatgcataaa
     3421 gatagtgaaa gtggaatcgt ttcatatgat gaagagaaat gtggttcatg ttatatgtgt
     3481 gtaatgtctt gtccatatgg attattaaag ccagatgata gaagcaagca gaacatactt
     3541 aaatgtgatt tatgtaaaga tgaggagtat cctagatgtg tagctaattg cccaagtgga
     3601 gcaattgaat tacagaagga ggaaaatgat gaattatgta gtgttagggg ctagtgcagc
     3661 tggtataaat gcagtaaaaa cattaagaga attagataaa gattcaaata tagtagtaat
     3721 ttctaaagat gaaaacgtat attcaagatg tatgttgcat cacgttatat ctgaacacag
     3781 aactttaaaa caaataaatt ttgttgatga agattttatg gagcaaaata atgtaaaatg
     3841 gattgcaggt aagactgtta aaggtataga cataaataaa aaggtagttc agacagaaga
     3901 cattactgta aattatgata aacttttaat agctacaggt gcatcttctg ctattcctcc
     3961 tataaaaaat ttaagagatg gaaattttgt atattctgtg agaaacatag atgatattta
     4021 taaaataaaa gaaaaagcac aaaattctaa aaatgtagtt ataataggtg ctggtttagt
     4081 tggtatagat gcattagtgg gattatttaa atatgagcag ttaaatattt ctgtagcttt
     4141 tatggaaaag tacattttag atagacaatt ggatgaatat acagcatcag tgtatgaaaa
     4201 taagttcaaa gaaaaaggtg taaaatttta cccaagtgca tcaatacaag aaattgtatt
     4261 ggataacagt aaaaatgtta caggtgttgc attttcaaat ggtgaagttt tagatgcaga
     4321 catggtaatt gtagctacag gggttaaacc aaatgctgac tttttagatg gaacaggaat
     4381 agaatatgat agaggtataa taatagatga tatgtgccaa acaacacaaa aagacatata
     4441 tgcagcagga gatgttgtag gtaaaaatgc tatttggcct ctggcagtaa agcaaggaat
     4501 tgtagctgcc tataatatgg ttggaaaaga taagaagata gaagatgaat ttgcttttaa
     4561 aaatagtatg aatttcatgg atataccaac tatttctata ggaatgaata cacctgtaga
     4621 tgatagctat aaagtattaa ccagacatgg aattaatgat tataaaaagt ttgtttttaa
     4681 agataatgta atctatggag cagttataca aggagatatt tcatatgtag gtgtattaac
     4741 ttatttaata aaaaataaag ttgaaatata tgatttagaa aataggatat ttgatatatg
     4801 ttatgcagac ttttttaata taaaagaaaa tggagaattt tgttatagtg tatagtatat
     4861 acgtggagta aggtttataa atacagtaaa tcaataaata gagttattct aaaataagaa
     4921 aaatgacaaa tcttatttag aaatagctct attttttgta attaaattta aattatattc
     4981 aatttgtaaa atattatttt gaacaattat acatatactc tctagttaga ggtaaatcat
     5041 tttttatacc tttagtgaaa agtatttgat gtatatctat aataccagtg ttgaaagcag
     5101 cagcacaaga acaaaggtat aaatcccaca ttcttataaa cttatcatca aacatttctt
     5161 taatctttgg aagattctca gagaaatttt tttgccagta tagtaaggtt tttacataat
     5221 gtaatcttaa actttctacg tctatagtat gatatttgta atcactacaa atatgtatca
     5281 tttctcttag gctaggaata actcctcctg ggaaaatata tttttttatc caagggtctc
     5341 cataagattc ctgcaatcca cttatatagt gaagtaaaaa caatccacca tcttttaaaa
     5401 cagcatttac gtttttaatg aaaagttcat agttttctct accaacatgt tcaagcatcc
     5461 caacgcttac aactctatca aacataaggt ctgatttttt taattctcta tagtccataa
     5521 gtcttacctc aagatagtct tgaaggttct cttctttaat tcttgcgtta aattccttaa
     5581 attgctcttc gctaagtgta attccaacgc cgtgaatttt atattttttt gcagcttcta
     5641 ttagtaaaaa tccccaacca caaccaatat cgagaaggct cataccttcc tctaaattta
     5701 gtttagttaa tatgtggtga actttattga cttgagcatc ataaagagta tcattatcat
     5761 ttctaaagta tgcacaagaa taactaagtg tcttatctaa ccataagcta taaaaatcgt
     5821 tgccaatgtc atagtgggca tgtatttctt tcttttgatg tctcattgaa tttgatgagt
     5881 gcaatagatg ttttacagct gaattatcaa tattgaattt gtctatttgt tttaaaagta
     5941 gatttaaagt taagaataaa tctccctcaa cttcaatgtc tctatttatg taggcttctc
     6001 caagtgctaa agatgtactt tttaatagtt cagacttgct aatatcatta tttactataa
     6061 ctttgaactg aggggagccc tctccaatta gaaattcttc ctcatcttta agcctaacta
     6121 gaaatggtgt ttcaccaaat ttctctaaat agtcttttaa aaaatttttt gtaatattca
     6181 tattaccagc tccttctaat gtaaatccag taagtttccc tgcttaaaat atattatagt
     6241 ccttttttta aaaaagtaaa attttttcaa taaatttttt atttaaagga aatttatttt
     6301 ttgtaaagaa atttcatgtt gtagtatatt ttttgcttat aaattttggt actggatata
     6361 atattaaact gttaatgata taataaattt aatctataaa gtaaataaaa ttttgtaata
     6421 aatagttaaa gctattttat aaggagatat atgatggaag tatatgcttt ggttgggtca
     6481 agtggcacag ggaagagctt taaggcttta gagttcgcat atgaaaatga tatagaatat
     6541 ataattgatg atgggatttt aatatacaaa aacaaggttt tagctgggat atcggcaaaa
     6601 caagcaaata ctactataga agcagttaaa agggctattt tttataatct ggagcataga
     6661 caagaagtta gagaagcaat taaaaaagaa aatataagaa aaattcttat tattgggaca
     6721 tcaaaaaaaa tggttaatca aattgtagag agattgtcaa ttggaagggt ttgcaagttt
     6781 ataaatatac aagatataag tactaaatat gaaatagaaa tagcaaaaca agctagaaaa
     6841 gaaggaaatc atgtaattcc tgttccagca gttgagataa aaagtatggc aagtggtctt
     6901 agtataaatt ctttgaagag actttttaga aaaggaaata ataggaatat ggtagtttta
     6961 gaaaaaacaa ttataagacc aacttttagt tatataggaa agttttatat tagtgctgat
     7021 gtaattaaac agattattga atatgaagta tacaactttg gaagcattga tagaataaat
     7081 aaaatcaata tagagaattg caataatttt atgaatattt ttgtaagtat aaatatcaat
     7141 gatttagaaa ctataaaaaa aattgaagac atacaacaga caataaaaaa gtctattgag
     7201 aaaatgactt tagttaatgt acaaaaaata gatattaaca ttcataaaat taaaaaaata
     7261 aacttcctcc aatgttaaaa aataaactga gaaaatgata tactaatttg caataaagtg
     7321 tgctataatt aaactgtaaa tttaggaaat ggttttttct aaatggagta tacataatta
     7381 taacgtattt ataagggggc tttcaaatgt caggaaaaga tgtaaatgtc ttcgagatgg
     7441 cgcaatctca agtaaaaaat gcatgtgata aattaggtat ggaaccagca gtttatgaat
     7501 tattaaaaga acctatgaga gttatagagg tttcaattcc agtaaaaatg gatgatggtt
     7561 ctataaaaac ttttaaagga tttagatcac aacataatga tgcagtaggg ccaacaaaag
     7621 gtggaataag attccatcaa aatgtttcaa gagacgaagt aaaagcttta tctatatgga
     7681 tgactttcaa gtgttctgta acaggtatac catatggtgg aggtaaaggt ggaataatag
     7741 tagatccatc aactttatct caaggtgaat tagaaagatt aagtagagga tacatagatg
     7801 gaatatataa attaataggt gagaaagttg acgttcctgc accagacgta aatactaatg
     7861 gtcaaataat gtcttggatg gttgatgagt acaataaatt aactggacaa agttctatag
     7921 gtgttataac tggtaagcca gttgaatttg gtggttcttt aggaagaaca gctgctactg
     7981 gatttggtgt tgctgttact gcaagagaag ctgctgctaa attaggaata gatatgaaaa
     8041 aagctaaaat agctgttcaa ggtataggaa acgttggttc ttatacagtt cttaactgtg
     8101 aaaaacttgg tggtactgtt gtagctatgg ctgaatggtg taaatcagaa ggttcttatg
     8161 ctatatacaa tgaaaatggt ttagatggtc aagctatgtt agattatatg aaagaacatg
     8221 gtaacttatt aaacttccca ggagctaaga gaatatcttt agaagagttc tgggcttcag
     8281 atgttgatat agtaatacca gctgcattag aaaactctat aactaaagaa gttgctgaat
     8341 ctataaaagc taaattagtt tgtgaggctg ctaatggacc aactactcca gaggctgatg
     8401 aagtatttgc tgaaagagga atagttctta ctccagatat attaactaat gctggtggag
     8461 ttacagtttc ttacttcgag tgggtacaaa acttatatgg atactactgg tcagaagaag
     8521 aagtagagca aaaagaagaa atagctatgg ttaaagcatt tgaatctatt tggaaaatca
     8581 aagaagagta caatgttaca atgagagaag ctgcttacat gcattcaatt aagaaagttg
     8641 ctgaagctat gaaattaaga ggatggtact aagatttact cttaataaat aataggatat
     8701 aattatttaa gatataaagg ggctgtacat agtacagccc ttaaattatg tggcttacaa
     8761 tgaagtaagc tatttttgtt ttaacttata aataacttga taaattatgt aatttagaat
     8821 gtatttttta ccaccacatg attacaaatt agaggagtta gattgaattt ttagacatga
     8881 acttatgcat tttaaaagca aagaccttaa tttaaagtat ctagtattat ttttaaaaat
     8941 agtatattgg tttaatccat ttatatgtat aatggaaaaa tatatggatt tggattgtga
     9001 attttattgt gatgagagag ttttaaaaaa ttgtactgta caacaaaagc aagaatatgc
     9061 tctaactatt gtaaattcaa tgaaaagagg tacaaatttt tcaaataaat ttgtagctgg
     9121 attaaataaa caatctgata ttataaaaag agtaatgaac atgttcagtg aaaaacataa
     9181 aaatggtatc ttaatggctt tgattataag cttgttttct tctattacat tttttaaatt
     9241 cgattcaact agtattattg aagagaatga tttcttagag aggttaagat atacgtttga
     9301 aggaaggcaa gaagatatgt ttgtaacaat agattttaca tatgcagata cacctataaa
     9361 acttagaaaa gagcatgaag aaaactgtag agtcaatgga atagtaccaa aagattcaga
     9421 ttcaatagaa attattccta gattttataa agattgtatg gatttaaaaa aataagttag
     9481 acttaatatt gatgttgata ttatgaattt tttatattta agcaattgaa aattttatta
     9541 tagattcaat tttatatgtg tttaagtatg aaatttatag atagttgaaa taattaaata
     9601 tgattgatgc ataggaatca ttactaagct ataattaatt ataggggagt ttaaataaat
     9661 tatgagttac acaattataa aatgataaaa atagttatct gttacaaaaa atgtatcttt
     9721 ttaattaata aagataatat gaaaacttaa aaggcaaata aacttaaata taaaatactc
     9781 ttaaataaga taggagaaaa atgtatgtct aactttattc taaatgggat ttttagaaca
     9841 gttatagtaa gtagtctaag cattcttctg atattgattt tcaagaaaaa tgtatttaaa
     9901 agatttagca aaaaatttaa ttattatata tggatgatag ttgttattaa attattttta
     9961 ccatttacat attatacttt tacaataaat atcttgagaa gtaaaaaaca cattaatatt
    10021 aataacataa atttagaggg atttaat
//
LOCUS       DAEOSP010000145         9760 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.147, whole genome shotgun sequence.
ACCESSION   DAEOSP010000145 DAEOSP010000000
VERSION     DAEOSP010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.147"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            43..1509
                     /gene="dndC"
                     /locus_tag="KRH54_002702"
     CDS             43..1509
                     /gene="dndC"
                     /locus_tag="KRH54_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation system sulfurtransferase
                     DndC"
                     /protein_id="HBG8580343.1"
                     /translation="MAVNYLDNNNLENLYDEMQAVYLNDDRPWIIGYSSGKDSSCVVE
                     LTFRMLKRLPKEKRHKEIYVISSDTLIENPIILDYLKNNIDKINESATEHDLPISAQI
                     VYPELNDSFWANIIGRGYPTPKSIKYRWCTERLKIKPSNKFIKEKLEQKDVIVLLGVR
                     KEESSARKSRIEKREIEGYLLTPHETLRGKNKLAYVYTPIVNFSTADVWDVLYHQNDN
                     FIDFGGEFGPSPSTSWGTDAMELFQMYMKGSGDSDECPFIADEASAKSSCGNSRFGCW
                     ICTVVKEDKSLNGFIESGHDELKPLLEFRSWLISERDKPKNRKKHKRNGSVIKKDGRI
                     MFGPFTFEARQTILRKLLETQLEMQKFYPDLQLIQLGELKAIDDIWDNEEDLTGNILS
                     KIYKEVVGKNLPWSEYKKPVFDDLTLNIISEKCSEYDINIDLFNKLMIDTNKYKYFSN
                     NTKLKTSIERILNQQWLHQDIIEKIEEESNKELKYENK"
     gene            1496..3655
                     /locus_tag="KRH54_002703"
     CDS             1496..3655
                     /locus_tag="KRH54_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG8580344.1"
                     /translation="MKINKLVLKNFRSYEEETTFNLNTTSEKNIILIGGKNGAGKSTI
                     FEAIKLCIYGPIAYKYQGFNSSYISRVKSNINNNSLRNSKVDTLVSVDIEISEGTEKN
                     TYTLIRQWTFKDKKLNETFSVYKNFSFTPLNADELNYFENYITSIISPKIFDFFFFDG
                     EHLYDFFIGKNSNIHLKESLLSLCNFDTFDILKKMLISTNRSNKNVSDEINIIKDDYL
                     NLEVEINNLNSQEKNLSYELEKISSEIEKLQQEQLKIKKEFRKKGGILAEEREMLNSR
                     IAELEGNRFIINQNIKDFCNDILPFLIVKKQILNLEKQLKVENDSILYSHLKDKLNIE
                     YIKNLLSDKIMSSSLDEIALTVSEALIEDIKPDSYEDNFESIHKLSNDESSKLLSLIN
                     TILNFDNNSIINSFGEHRSISNELADIRHKLNTSFKDETLNTYINDISSLSDKIDSLL
                     KSKNLLVTSLDKLRLEIAETITKRDKSKAKYIYLLQHNNIVDISSNLILMLDEIITTL
                     TETKIKLVQDNFMYIFKRIIQKDNFISSIDIDANFNISLYINKIYNSLELENLVENIG
                     SEEMEKKLGKLFFEDLFNLYRVKDKDDLFNAIKNNTQSTFLNLRTKVDIDEFSSGEKQ
                     IYILCLYWALLKSSEIEIPFIIDTPYARIDDTHRNNITSEYFSTISDQAIILSTNTEI
                     DEKSYKIIKPKLSNEYLIEYDNKNKKTIQNKGYFFEV"
     gene            3658..4041
                     /locus_tag="KRH54_002704"
     CDS             3658..4041
                     /locus_tag="KRH54_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DndE family protein"
                     /protein_id="HBG8580345.1"
                     /translation="MGFRLKTSIQTKEIFEELGSRMNLKPFALCKLAISISLSNSEPI
                     ENYESIDNNGLELNRQTITNQYDTLFKTLLEQHAGRHLSDDEYFPNQVKLHIDRGALM
                     LKNRYNYSSNLEHFITQIVQENNNL"
     gene            complement(4151..5293)
                     /locus_tag="KRH54_002705"
     CDS             complement(4151..5293)
                     /locus_tag="KRH54_002705"
                     /inference="COORDINATES: protein motif:HMM:NF014044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8580346.1"
                     /translation="MKIIFGNIKNNNKELIWDPTNSIKVPHPNTGIIGTMGTGKTQFT
                     KSLVTQIHREQKHNINGKPIGILIFDYKGDYIKDDFVDATSAKVYEIYHLPYNPLTLI
                     IPERIKPLLPVHTANSLKETIAKAFGLGPKQEVILRELIMKAYEKKGIKKDDENTWVK
                     SCPTMQDVLSIYLDDREIKKDDKLYAALSSFNDFEIFEPNSEKTKNLFDLINGVTVIN
                     LSYYDSGIQNLVVAITLDLFYSQMKALGSSDITGNGEYRQLNKMILVDEADNFLSRNF
                     TSIKNILKEGREFGVGTILSTQLLSHFSTSENEYQNYISTWIIHNVSDINNKDIRYIF
                     NSKNKSEEDNIYNKIKNLAKHESLVKMPSENSPIYIRDKAFWELNK"
     gene            complement(5303..9274)
                     /gene="dptH"
                     /locus_tag="KRH54_002706"
     CDS             complement(5303..9274)
                     /gene="dptH"
                     /locus_tag="KRH54_002706"
                     /inference="COORDINATES: protein motif:HMM:TIGR03237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptH"
                     /protein_id="HBG8580347.1"
                     /translation="MSDQFYRYLAKNLVKYFESREIKSGDKFNIQFEREEQVKIMYDV
                     LEKESNSRPFTYNIMNGSKYQTFSMNVSGIQLIVASNNNTTNDFLIRLRNLIGNVASD
                     EFKDSAILFIHNMTLDSLIRGTESLQKEGMPFHTKTLVKDIQNKLENDKLKEDIKHIL
                     YFVLDRMNKSILEDNSSIFQYENILDILNKGRIEKSEYSKFGLFYDDNMLDNKSSKAI
                     KERLLDNEKLFSIVNSIHQYGDPKLELEKHFDSKGIDLLKDENWKYSTYKDIKSSQER
                     MKNKKVELLCIECVENLKLWSKPDIENKSKRKNILLFNKDKLDIINLCIKFDKKIEKT
                     SFSIDKMSKTYTEVKASGNKAYISININISETTFTKVKYNDGIIKYEFRICIVSCDEN
                     ILNDIKSNYGIVINSKRSMLVTNTDDDTIVINPKGIIERKIQIDEFENEINLEEGERL
                     DLELNVELEEDVEFIKIDVVYDQSVITIVKQEEKEQPISISGMKTWKLKRERKESFKY
                     LGNSKIVQGTSEYFPKENFKENLQIEKYIIENNIMHCTILEEKYKIIELDVDSRIKEA
                     YKNLISYYKVNDCIPSLAYYDVDLVNISKNYINTILEVFEDIKNGKVLSKERLNIAKV
                     GTIHRIDKEEELFLTPLHPVNVAYQLMINNVIGDEEVKEGILKLLGSKYLMPYIYKGK
                     NSLYKVVDQRHSPEWTYYVDHDMSRYKGSRDYVSKLTKEKINEFIEHFTYLFRDISKS
                     VMKIKLINLGDCKEVLLGIFDYYIKKLGKGEEINNLISIDIYIYGDKSIINSFEEIAF
                     YNSINDIKKHFNVNLSVDNYSEEDVLNLFRDKVKFYKKDKMSDLYEYSHLTLYQMDKD
                     VNITSSSMDDIKTGVALNGLIAGVPSIYLEDTYRTGFGSKFMATTKNELLNISMKFNS
                     LTKSIGTGDPFNDNLSVTTSISENNSSFLNKIYKTSHWVTFIDPKVDLNFFKDDENSN
                     DLLIIHYSDQYTTSSGYDAITVTRKSEQYKFIIEEFLNSKDIKVEEDTIPNLINYFNA
                     INGDWLLRLISSKSQFPREKLSILSAIKISLAYFYHPNIIWIPISLEEILRVSGGAGL
                     KQAGGLFTVKKLTGDTGSYSDDLLLVGIENKNNKIKVYFYPVEVKIGDNNESVIKKAI
                     IQANKTRDLIEEYLTESENDSQKFTKLMYRNFMIQLAIVSAQKMKLYHIWDEQNWDDI
                     INSDIRTNLLNDNYEISNDLDEFIGRGAVISFKKGTYYNGNLGKEGNITILNLSEQEG
                     YNNIVMDLEEMKEKYIRGESDFVIQEMLINKYLDNTIGIQNMDKNVILDTNVMLNKTK
                     DYD"
     gene            complement(9255..>9760)
                     /gene="dptG"
                     /locus_tag="KRH54_002707"
     CDS             complement(9255..>9760)
                     /gene="dptG"
                     /locus_tag="KRH54_002707"
                     /inference="COORDINATES: protein motif:HMM:TIGR03236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptG"
                     /protein_id="HBG8580348.1"
                     /translation="KNLSENERLRLYENIIDIKNTYVKSITDVQWDDYDEQTKVYKND
                     NIKEEICDLHKRIDYQFKKSSREANYKRYKNLFEEYCKLNFLKKAGPLGNVLKINQEE
                     LLFMTKLCMKNKTKIKLKELFEEYEKRGIYFDRDSQSKVVELFEKLNIIEKKSDSGDA
                     QYVRSIL"
BASE COUNT         3448 a         1401 c         1070 g         3841 t
ORIGIN      
        1 taaagatctt cgctatactc ttatagaaag gatacttaat atatggctgt taattaccta
       61 gataacaaca atttagaaaa tttatatgat gaaatgcagg ccgtatatct taatgatgat
      121 agaccttgga taatcggata tagttctgga aaagatagtt cttgtgtagt tgaacttaca
      181 tttagaatgc ttaaaagact acccaaagaa aaaagacata aagagattta tgtaatctca
      241 tctgatacac ttatagaaaa tccaataata cttgattatc taaaaaataa tatagacaag
      301 ataaatgaaa gtgctactga acatgattta cctataagtg cacagattgt atacccagaa
      361 cttaatgata gtttttgggc caatataatt ggtagaggtt accctactcc aaagtctatt
      421 aaatatcgtt ggtgtactga aaggcttaag ataaaaccat caaataaatt tataaaagaa
      481 aaacttgaac aaaaagatgt tattgtactg cttggcgttc gtaaagaaga aagttctgct
      541 agaaaatcga gaatcgaaaa aagagagata gagggttacc ttcttacccc acatgaaacc
      601 ttaagaggta aaaataagtt agcttatgtg tataccccta tagtcaattt tagtacagca
      661 gatgtttggg atgtactcta tcatcaaaat gataatttca tagattttgg tggtgaattt
      721 ggaccatctc catctacctc atggggaact gacgctatgg aattatttca gatgtatatg
      781 aaaggttctg gtgactcaga tgaatgccca tttatagcag atgaagctag tgctaaaagt
      841 agctgtggaa atagtaggtt tggatgttgg atttgtactg ttgtaaaaga agacaaatca
      901 ttaaatggat ttattgaatc tggtcatgac gaattaaagc cacttttgga attcagaagt
      961 tggctaattt ctgagagaga taaacctaaa aatcgtaaaa aacataaaag aaatggctct
     1021 gtcatcaaaa aagatggtag aatcatgttt ggaccattta catttgaagc aagacaaact
     1081 attttaagaa aacttttaga aactcaactt gaaatgcaaa aattttatcc tgatttacaa
     1141 cttattcaat taggagaact taaagctata gatgatattt gggataacga agaagactta
     1201 actggtaata tattatcaaa aatatataag gaagttgtag gcaaaaacct accatggtct
     1261 gaatataaaa aaccagtatt tgatgacctt actttaaata ttatatctga aaaatgtagt
     1321 gagtatgata taaatattga tttatttaat aaattaatga tagatacaaa taaatacaaa
     1381 tattttagta ataatactaa attaaaaact tccatagaaa gaatattaaa tcaacagtgg
     1441 cttcatcaag atattattga aaagattgaa gaagaaagca acaaggagtt aaaatatgaa
     1501 aataaataaa cttgtattga aaaatttcag atcttatgag gaagaaacca ccttcaatct
     1561 aaatactact agtgaaaaaa atattatatt gataggtggt aaaaatggtg ctggtaaatc
     1621 cacaatattt gaggctataa agctatgtat atatggacct atagcttata aatatcaagg
     1681 tttcaactct tcatatatat ctcgagttaa atctaatata aataataatt ctctaagaaa
     1741 tagtaaggtt gacaccttag tatcagtaga tatagaaatc agtgaaggta ctgaaaaaaa
     1801 tacctatacc ttaattagac aatggacttt taaagataaa aaattaaatg aaacattttc
     1861 agtttataaa aatttttcat ttacaccatt aaatgctgat gaacttaatt attttgagaa
     1921 ttatattaca tctattattt ctcctaaaat atttgacttt ttcttttttg atggagaaca
     1981 tttgtatgat ttttttatag gtaaaaattc aaatatacat ctaaaagaat ctttattatc
     2041 tctttgtaat tttgatacat ttgatatttt aaaaaaaatg ttaatttcaa ctaacagatc
     2101 caataagaat gtttcagatg agattaatat tataaaagat gactatttaa atttagaagt
     2161 tgaaatcaac aacttaaact ctcaagaaaa aaatctatct tatgaattag aaaaaatttc
     2221 aagtgaaatt gagaagttac aacaagaaca attaaaaata aagaaagaat tcaggaaaaa
     2281 aggtggaatt ttagcagaag aaagagaaat gcttaattct agaattgctg aattagaagg
     2341 aaatagattt ataattaatc aaaatataaa agatttttgt aatgacatac ttcccttttt
     2401 aattgtaaaa aaacaaatat taaacttaga aaaacaactt aaagtagaga atgatagtat
     2461 attatattct catcttaaag ataaattaaa tattgagtat ataaaaaatc tactttcaga
     2521 taagatcatg tcttcctcat tagatgaaat agctctaact gtttctgagg ctttaataga
     2581 agatataaaa ccagatagtt atgaagataa ctttgaatct atccataagc tttctaatga
     2641 tgaaagtagt aaattattat ctttgataaa taccatttta aattttgata ataattctat
     2701 cattaattcc tttggagaac atcgaagtat ttcaaatgaa ttggcagaca taagacataa
     2761 gttaaatact agctttaaag atgaaacttt gaatacatat ataaatgata tatctagttt
     2821 atcagataaa attgattctt tattaaaatc aaagaatttg ctagttactt ctttagataa
     2881 attaagactt gaaatagctg aaactatcac taaaagagac aaatctaagg ctaaatatat
     2941 atatttactt caacataata atattgttga tatatcttct aatcttatac ttatgttaga
     3001 tgaaataata acaacattaa cagaaactaa aattaaacta gttcaagata attttatgta
     3061 tatatttaaa aggattattc aaaaagacaa ttttattagt tctatagata ttgatgccaa
     3121 ctttaatatt tctttatata taaataaaat atataactct ttagaacttg aaaatttagt
     3181 agaaaatata ggatctgaag aaatggagaa gaagttggga aaattatttt ttgaggattt
     3241 atttaactta tatcgtgtaa aagataaaga tgatctattc aatgctataa aaaataatac
     3301 tcaatctaca tttttaaatc taagaactaa agttgacata gacgaattct ctagtggtga
     3361 aaaacaaata tacattcttt gtttatattg ggctttactt aaatcttcag agatagaaat
     3421 accatttatt atagatacac cttatgctag aatagatgat actcatagaa ataatattac
     3481 tagtgaatat ttttctacaa taagtgatca agctataata ctatctacta atactgaaat
     3541 agatgagaaa tcatataaga taataaagcc taagttaagt aatgaatact tgattgaata
     3601 tgataataaa aataaaaaaa ctatacaaaa taaaggatac tttttcgagg tgtaattatg
     3661 ggatttagac ttaaaacatc catacaaaca aaagaaatat ttgaagaatt aggatctaga
     3721 atgaatttga aaccatttgc tctttgcaaa ctagctatct caatctcact aagtaatagt
     3781 gagccaattg aaaactatga aagcatcgat aataatggtc ttgaacttaa tagacaaact
     3841 ataaccaatc aatatgatac tctatttaaa actttacttg aacagcatgc aggtagacat
     3901 ttaagtgatg atgaatactt tcctaaccaa gttaagcttc atatagatag aggtgctctt
     3961 atgcttaaaa acagatataa ttattcaagc aacttagaac actttatcac tcaaatagta
     4021 caggaaaata ataatctata aattaattaa tatttattaa acataaataa attaaaacat
     4081 gggatagaca ataaataaaa ttgcctatcc catgttttaa ttataagtat tttttctaaa
     4141 ttcatttatt ttatttatta agttcccaaa atgctttatc tctaatatat ataggtgagt
     4201 tctcactagg cattttgact aagctttcat gtttagctag attctttatc ttattatata
     4261 tgttatcttc ttcactttta ttcttagaat tgaatatgta tcttatgtct ttattattta
     4321 tgtctgatac attatgtatt atccaagttg aaatataatt ctgatactca ttttctgaag
     4381 ttgaaaaatg acttagaagt tgtgttgata gaatagttcc tactccaaac tctctacctt
     4441 ccttcaatat attttttata gatgtaaagt ttctactcaa gaaattatca gcttcgtcta
     4501 ctaatatcat tttatttaat tgtctatact ctccatttcc agttatatca cttgatccaa
     4561 gtgccttcat ttgactatag aataaatcaa gagttatcgc aactactaaa ttttgtattc
     4621 ctgaatcata atatgataaa tttataactg taactccatt tattaaatca aataaatttt
     4681 tagtcttctc actatttggc tcaaatattt caaaatcatt aaaacttgaa agagcagcat
     4741 acaatttatc gtcttttttt atttctcgat catcaaggta tatcgataat acatcttgca
     4801 ttgtagggca tgattttacc catgtatttt catcatcttt ttttattcct tttttttcat
     4861 aagctttcat aattaattct ctcaaaataa cttcttgctt agggcctaag ccaaatgctt
     4921 ttgctattgt ctcttttaaa gaattcgctg tgtgaacagg tagtagtggc tttattcttt
     4981 caggtattat taaagttaat ggattataag gtaaatgata tatttcatat accttagcac
     5041 ttgtcgcatc tacaaaatca tcttttatgt agtcgccttt ataatcaaaa atcagtattc
     5101 ctattggttt accatttatg ttatgttttt gttctctatg aatttgcgtt actaaagatt
     5161 tagtaaactg tgttttccca gttcccattg ttcctattat accagtatta ggatgaggca
     5221 ctttaatcga atttgttgga tcccaaataa gttctttatt attatttttt atatttccaa
     5281 atataatttt cattaaacca cttcaatcat aatcttttgt tttatttagc ataacattag
     5341 tatctaaaat aacattctta tccatatttt gtatacctat tgtattatct aaatacttat
     5401 ttataagcat ttcttgtata acaaaatcac tctctcctct aatatacttc tccttcattt
     5461 cctctaaatc catcactata ttattatatc cttcttgttc tgaaagattt agtatagtta
     5521 tattgccttc tttgcctaaa ttcccattat aataagttcc ctttttaaat gatattacag
     5581 ctcctcttcc tataaattca tctaaatcat tacttatttc ataattatca ttcaacaagt
     5641 ttgttcttat atcactattt attatatcat cccaattttg ttcatcccat atatgatata
     5701 acttcatttt ttgtgcactt actatagcga gttgtatcat aaaattcctg tacataagct
     5761 ttgtaaactt ttgggaatca ttttcgcttt cagtcaagta ctcttctatt aaatctcttg
     5821 tcttattagc ctgaattatt gcctttttta tgacactttc attattatct ccaattttca
     5881 cttcaacagg ataaaaatat acttttattt tattattttt attttctatc ccaacaagta
     5941 ataaatcatc tgaataagat ccagtatctc ctgttagttt cttaacagta aacaaaccac
     6001 ctgcttgttt taatccagct ccaccagata ctctaagtat ttcttcaaga gatattggta
     6061 tccatattat atttggatga taaaaataag ctaagcttat ttttatagct gataatatgc
     6121 ttaatttttc tctaggaaac tgagatttac tagatataag ccttaataac caatctccat
     6181 ttatagcatt gaaataatta atcaaattag ggattgtgtc ttcttccact ttaatatctt
     6241 tactatttag aaattcttca attataaatt tatattgttc agattttcta gtaactgtta
     6301 tagcatcata tccactagat gttgtatatt gatcactata atgtattatt aataagtcat
     6361 ttgaattctc atcatcctta aaaaaattta aatccacttt tggatctata aaagtaaccc
     6421 aatgagatgt cttataaatt ttatttaaaa atgaactatt gttttcactt attgatgttg
     6481 tcacactaag attatcatta aatggatctc cagttcctat tgattttgta agtgaattaa
     6541 acttcattga aatatttaac agttcatttt tagttgtagc cataaactta gatccaaatc
     6601 cagtcctata agtatcctct aaatatattg atggaactcc tgctatcaat ccatttaatg
     6661 ctacacctgt ttttatatca tccatagagc tacttgttat atttacatcc ttatccattt
     6721 gatataaagt caaatgggaa tactcatata aatctgacat tttatctttt ttataaaatt
     6781 taactttatc tctaaataaa tttaatacat cttcttcaga gtaattgtct acacttaaat
     6841 ttacattaaa atgttttttt atatcattta ttgaattata aaatgctatt tcttcaaatg
     6901 aatttataat acttttatct ccatatatat aaatatctat tgatattaag ttatttattt
     6961 cctcaccttt acctaatttc tttatgtaat aatcaaatat accaagtaat acttctttac
     7021 aatcacctaa atttattagc tttatcttca ttactgattt acttatatcc ctaaataaat
     7081 atgtaaaatg ttctataaat tcatttattt tttcctttgt caatttagag acataatctc
     7141 tagatccctt atatctactc atgtcatgat caacatagta tgtccactct ggtgaatgtc
     7201 tttgatctac taccttatat aaactattct tacctttata tatatagggc attagatact
     7261 tcgaacccaa tagttttaag attccttctt taacttcttc atcacctata acattattta
     7321 tcattaattg gtaagccaca tttactggat gtaatggtgt taaaaataat tcttcttctt
     7381 tatctattct atgtatagtc cctaccttag ctatattaag tctctcttta gataatactt
     7441 ttccattttt tatatcttca aacacttcta atatagtatt tatataattt tttgatatgt
     7501 taactaaatc cacatcataa tatgccaagc ttggaataca atcatttact ttatagtaac
     7561 ttataaggtt tttgtatgct tcttttattc tggaatctac atccaattct attattttat
     7621 atttttcttc taaaatagta caatgcatta tattattttc tatgatatat ttttctattt
     7681 gcaaattttc tttaaaattt tcttttggaa aatattcact agtaccttgt acaattttac
     7741 tattacctaa atatttaaaa ctctcctttc gttctctctt taatttccag gttttcattc
     7801 cacttataga aattggttgt tctttttctt cttgtttaac tatggtaatt acagattgat
     7861 catatactac atcaattttt ataaattcaa catcttcttc caattcaaca ttaagttcta
     7921 aatctagcct ttctccttct tcaagattaa tttcattttc aaactcatct atttgtattt
     7981 ttctctcaat tatacctttt gggtttatta ctattgtatc atcatctgtg ttagtaacta
     8041 acatacttct ttttgaatta ataacaattc cataattact ttttatatca tttaatatat
     8101 tctcatcaca agatactata caaattctaa attcatattt tattatacca tcattatatt
     8161 taactttagt aaatgtagtt tctgaaatat ttatatttat acttatataa gctttatttc
     8221 cacttgcttt tacttctgta tatgttttag acatcttatc tatagaaaaa cttgtttttt
     8281 caattttctt atcaaatttt atgcataaat ttataatatc aagtttgtct ttattaaata
     8341 ataatatgtt ttttcgtttt gacttatttt caatatctgg tttactccag agctttaggt
     8401 tttcaacaca ttctatacat aataactcta cctttttgtt tttcattctt tcttgagatg
     8461 actttatatc tttataggta gaatacttcc aattctcatc ttttaaaaga tctatacctt
     8521 ttgaatcaaa atgtttctct aattccagct tagggtctcc atactgatgt atagagttta
     8581 caatagaaaa cagcttttca ttatctaata gtctttcttt tattgccttg ctacttttgt
     8641 tgtctaacat attatcatca taaaataatc caaatttact atattcactc ttttcaattc
     8701 ttcctttatt taatatatct aatatatttt catattgaaa tattgatgaa ttatcttcta
     8761 atatactttt attcattcta tcaagtacaa aataaagtat atgtttaata tcttctttta
     8821 atttatcatt ttctaattta ttttgtatgt cctttactaa agtttttgta tgaaatggca
     8881 taccttcttt ttgcaaactt tctgttcctc ttattaaact atctaatgtc atattatgta
     8941 taaataatat ggctgaatcc ttaaattcat cactagctac atttcctata agatttctaa
     9001 gtctaattaa aaaatcattg gttgtattat tatttgaagc aactattaat tgaataccac
     9061 tgacattcat actaaaagtc tgatattttg atccattcat tatattatat gtaaatggtc
     9121 tactattact ttctttctct aaaacatcat acataatttt aacttgttct tctctttcaa
     9181 actgtatatt aaatttatcg ccacttttta tttctctaga ttcgaaatat ttaactagat
     9241 ttttagctag atatctataa aattgatctg acatattgtg catccccact atcactcttt
     9301 ttttctatta tatttaattt ttcaaataac tctactactt tgctttgaga atctctatca
     9361 aaataaattc ctcttttttc atattcttca aataattcct ttaattttat ctttgtctta
     9421 tttttcatac ataattttgt cataaataac agttcttctt gattaatttt caatacattt
     9481 cccaatggcc ctgctttttt taaaaaattt aatttacaat actcttcaaa taaattttta
     9541 tacctcttat agttagcctc tcttgaagat ttcttgaact gataatctat tcttttatgt
     9601 aaatcacata tttcttcttt tatgttatca ttcttataca ctttagtttg ttcatcataa
     9661 tcatcccatt gtacatcagt aattgacttg acataagtat tttttatatc tattatattt
     9721 tcatataacc taagtctttc attttctgat aaatttttta
//
LOCUS       DAEOSP010000146         9632 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.148, whole genome shotgun sequence.
ACCESSION   DAEOSP010000146 DAEOSP010000000
VERSION     DAEOSP010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9632
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.148"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(225..497)
                     /locus_tag="KRH54_002708"
     CDS             complement(225..497)
                     /locus_tag="KRH54_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580349.1"
                     /translation="MYDEIIEIYFDYWINEHMYRDILEKDKYYIESWKEVKKINELLE
                     KVLEDDKKYVLVELNDLFNSLIEFERELSYKLGFKTGAKFIIELMD"
     gene            complement(745..2079)
                     /locus_tag="KRH54_002709"
     CDS             complement(745..2079)
                     /locus_tag="KRH54_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG8580350.1"
                     /translation="MNNSNNLIKNTEAEQSILGSILLDSTIVQELEQMLNEDMFYHEE
                     HKYIFNAIKEINKRNEVIDIITLTEELKRKGHLNNIGGISYITSLSTIVPTVSNFMSY
                     VSIVIEKYKNRNIIDKFKKFNLGEIDDNTLIYQLENDINKANQKLIKKDSSITAISER
                     AFEYINDEVNEGIKTGIKFLDDTIGGLYGGELTTIAAKSGRGKTALALQILRNVIFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNDEEIVKYIQALSIINQENNLY
                     INDSVNTISGIKREIKSIKPSLVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPVIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALNYCKTMGIE
                     DIRELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGNLFSFEECKKIQEI"
     gene            complement(2092..2835)
                     /locus_tag="KRH54_002710"
     CDS             complement(2092..2835)
                     /locus_tag="KRH54_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580351.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKRIKIKSGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSRIKEYDKETVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYNSKEIVINKETGIYNPDDSQLKW"
     gene            complement(2857..3171)
                     /locus_tag="KRH54_002711"
     CDS             complement(2857..3171)
                     /locus_tag="KRH54_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580352.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            complement(3175..3375)
                     /locus_tag="KRH54_002712"
     CDS             complement(3175..3375)
                     /locus_tag="KRH54_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8580353.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKED"
     gene            3522..4286
                     /locus_tag="KRH54_002713"
     CDS             3522..4286
                     /locus_tag="KRH54_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8580354.1"
                     /translation="MQIGKNLKNIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYLLQSLFPEEYYSFDEFLNAISNLME
                     DDDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCISNTELDFSLHPDDI
                     KEIIKNDVLTKQSIDELLDIGLSEENIETIYDYFSDSTDNGSNIRLKKLISQKENITI
                     KNNDYFKLLQENNELKEKNEKLENTLKEIKKLLTKI"
     gene            4343..4541
                     /locus_tag="KRH54_002714"
                     /pseudo
     CDS             4343..4541
                     /locus_tag="KRH54_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729178.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4538..5029
                     /locus_tag="KRH54_002715"
     CDS             4538..5029
                     /locus_tag="KRH54_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8580355.1"
                     /translation="MMNNTIGEKIKQYRKSINMSRDELANKIGCSVHAIAKYEQNQRM
                     PSLDMIRKIATALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVIHILENYGYDI
                     KTDYKKKFEITDNADTIKPINLNFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            5121..5873
                     /locus_tag="KRH54_002716"
     CDS             5121..5873
                     /locus_tag="KRH54_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG8580356.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVNDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            5863..7029
                     /locus_tag="KRH54_002717"
     CDS             5863..7029
                     /locus_tag="KRH54_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBG8580357.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNSDILKAGELYRNDNYVFCNELGIPIDDKRPLRNLKSILNSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(7145..7375)
                     /locus_tag="KRH54_002718"
     CDS             complement(7145..7375)
                     /locus_tag="KRH54_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580358.1"
                     /translation="MEANKTLLQILYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGVADMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(7381..7860)
                     /locus_tag="KRH54_002719"
     CDS             complement(7381..7860)
                     /locus_tag="KRH54_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HBG8580359.1"
                     /translation="MIVYHGSYCVVKTPSITFSRDSLDFGKGFYVTSIKTQAINWTER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFNSYNLEWLDFIIECRNMSDIYMKYDIIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRYEEEV"
     gene            complement(7860..8075)
                     /locus_tag="KRH54_002720"
     CDS             complement(7860..8075)
                     /locus_tag="KRH54_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HBG8580360.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKGYIVEDLMQVLKEKGVI"
     gene            complement(8391..8740)
                     /gene="ssrA"
                     /locus_tag="KRH54_002721"
     tmRNA           complement(8391..8740)
                     /gene="ssrA"
                     /locus_tag="KRH54_002721"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(8898..9353)
                     /gene="smpB"
                     /locus_tag="KRH54_002722"
     CDS             complement(8898..9353)
                     /gene="smpB"
                     /locus_tag="KRH54_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBG8580361.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
BASE COUNT         3442 a         1460 c         1083 g         3647 t
ORIGIN      
        1 tccaactata caaaacttat ctaatataat tataattttg tatagtataa agagttgata
       61 acatataaat attatagtga ctatgatgaa ttcaaaaaaa tcttaaattt caaatataaa
      121 aatatattgt atatagtata ctaatataat gctaaattaa aaaggacact tttaatgtcc
      181 tctttaattt agcattatag aattaactta taagcataag taaattaatc cattaattct
      241 attataaatt ttgctcctgt tttaaatcct aatttataac taagttccct ttcaaactct
      301 attaatgaat taaacaaatc atttaattca accaacacat atttcttatc atcctccaaa
      361 actttttcta ataactcatt aattttttta acctctttcc aactttcaat ataatatttg
      421 tctttttcta aaatatctct atacatatgt tcatttatcc aataatcaaa atatatttct
      481 ataatttcat catacatttt agtacctcct tgactttcct caggcactta atatatactt
      541 aagttgctag gttagtgtac atcaagtacc taatttataa aggtagttgt tatatttata
      601 tggcaactac ctttttctaa taagtgttta aatctaaact aaatatattt taaatcttta
      661 ttacatctaa ttaaaattta tatttatact tcaacacttc ttgaaacata atttatctat
      721 tacttaataa atcatgcttc ttttttatat ctcttgtatc ttcttacatt cttcaaaaga
      781 aaacaaattt cctatatacc aatatggtct aaatcctgac tcgccatctc tatttttagc
      841 aacaataatc tcacttaaat tagcaccaaa acctttttct cttcgtctca actctcttat
      901 atcctcaatt cccattgtct tacaataatt aagagcttct ttttctgtag gtctatgaat
      961 atatataact gaattagaat ccatatatat ctgctttgag cttctcatca ctctatcacc
     1021 tttaggtctt tcatctttca attcatcatt taattgagca agctgaataa ctggtatatt
     1081 aaaatctaaa gtaatctttt ttatttgcct tgataagtca gcaaatttag cttctcctac
     1141 ttctttacct tcataatcca aaatttgcaa ataatcaatt atgactaagc taggcttaat
     1201 actctttatt tctctcttta ttcctgatat tgtatttaca gagtcattta tataaagatt
     1261 attttcctga tttattatgc ttaaagcttg tatatatttg actatttctt catcatttag
     1321 atttctattt ttcatcttta caactgatat tccagttagt gatgctatat ttctaaatag
     1381 aatctgtata tcgctcattt ctccacttat aaaaagtact ttcttacctt gaaatattac
     1441 atttctaagt atttgtaacg ccaatgctgt tttacctctt ccacttttag ctgctattgt
     1501 agttagttca cccccatata aacctcctat agtatcatct aagaacttta ttccagtttt
     1561 aataccttca tttacttcat catttatata ttcaaaggct ctctctgata tggctgttat
     1621 acttgaatcc ttttttatta atttctgatt agctttattt atatcatttt ctaactgata
     1681 tataagagta ttatcatcta tttctccaag attgaacttt ttgaatttat caattatatt
     1741 tctatttttg tatttttcaa taactatact tacataactc atgaaatttg atactgttgg
     1801 aactattgtt gacaaactag tgatatagct tatacctcca atattattca aatgaccttt
     1861 tctctttaat tcttctgtaa gagttattat atctattact tcatttctct tatttatttc
     1921 ttttatagca ttaaatatgt atttatgttc ctcatgataa aacatgtctt catttaacat
     1981 ttgctctaac tcttgtacta ttgttgaatc taataaaata cttcctaata ttgattgctc
     2041 tgcttctgta tttttaatta agttgtttga attatttata atataacctc cttaccactt
     2101 taattgtgag tcatctggat tatatattcc tgtctcttta tttatgacaa tttccttgct
     2161 attataattt ccctctagta tcttagtaaa attacttgaa ttcattatcc aatcaaaatt
     2221 tgctttccat cctgtattat tatttccatt cagaaaactt gttttactaa ctatatttat
     2281 aacctctaat acagtttctt tatcatattc ttttattctt gaatttattc tcctctttct
     2341 tgtatctgtt agtttcttga cctttgaaag ttcaatacaa gttgagttaa atttgcttat
     2401 gatttcacta tatataatat tattttcatt ctttatattc ttgattgttg ttgattgatt
     2461 gttaatagtc tgttgattag gttgttgatt ttctatgttc tcttgataaa gctcccagtt
     2521 ttcaatagtt atgactatat acttgtttgt tgtttcgtat gttagaaaac cagcattttt
     2581 aaaaaccaac aaacaacttc taaccatttg ataactaact cctttaccac atctttcagc
     2641 tattttgcta taggaagaaa taaattgtcc agattttatt tttatccttt tattatctat
     2701 aaaaacatgg ttttctttat gactagccat aagtaataga gttattaata ttacttttct
     2761 ttctggttta atgtctttcc aaattgattt atccaacaac tccctataaa gctttatcca
     2821 ccctttattg ctcaattaca aacacctact ttaaggctat aacttgatac catttgcatt
     2881 ttcaataaac caatcatcta atttattttt tattatgtat actcgattat ttatccatat
     2941 acaagggaat cctttgtttt tagaatgaca taactgccta aattttgatt ctcctatagg
     3001 ataaagctta gatgcttctt tacatgttat aagttcagct ttatttcttt tcgcattttc
     3061 aataatttta tatccttgct ttttttcttg tgcttcttct tcattcttta caataatgtt
     3121 taaagtggct tgtaatgtct ttaaaacctc taaattatta ttctctttca tattttaatc
     3181 ctccttacta aaaaataatt cttctactgt tgtatctaaa gctctagata gtttaatcat
     3241 caaatcattg cttggattat tagccttacc tgtttccaag tttgataaat aatatcttga
     3301 tataccaact ctatctgcca gttcttgttg ctttagacct tttttaatcc taaatatttt
     3361 taaattgatt cccatacttt ttcctccttt tttctagttt acattcgtaa actttacatt
     3421 ttaattataa tgtcgacata tgtgtactgt aaataaaaat atattatttt tctgttctca
     3481 tttgtgtact tttgggctat actttatacg aggtgataat attgcaaatt ggaaaaaact
     3541 taaaaaatat aagaaaacaa aaagaactaa cgcagattca acttgctgaa attagtggca
     3601 tctcaagaaa tgctctaata aattatgaaa atgataagag aatacctagt atagatacct
     3661 tatcaaaact cgctaaagct ttgaaaattg aaaagactat cttttatttt gatattgaaa
     3721 atgaagtaat gactaatgaa gatgttttat accttttaca gtctttattc ccagaagaat
     3781 actattcttt tgatgaattt ttaaatgcta tttcaaatct tatggaagat gatgatatta
     3841 gaattacctc aagtttattg ctaggtgcct ggtctgataa agaccaatat tataaacttg
     3901 gtgaaaaatt agaactaaat gaaaatcaag tttataattg gatactttca gattgtatat
     3961 ctaatactga attagatttt tcattacatc ctgatgacat aaaagaaatt attaaaaatg
     4021 atgttcttac aaaacaatca attgatgaat tattagatat tggtctatca gaagaaaata
     4081 tagaaacaat ctatgattac tttagtgact caacagataa tggaagtaat attcgactaa
     4141 aaaaattaat atctcaaaaa gaaaatatta ctattaaaaa taatgattac tttaaattat
     4201 tacaagaaaa taacgaacta aaggaaaaaa atgaaaagct tgaaaataca cttaaagaaa
     4261 taaaaaaact actaactaaa atataaaata atatcaatac tatctaaagc aatgataaaa
     4321 aactatgaat ggagttacaa ttatgaataa atcatatatg ctaaaataca catataaatg
     4381 ccctactaat ttatttcatg gagataaaaa atatactcta acatacacta agagagttat
     4441 gagtaattta gtatatgata tgggataatc tagaaaataa ctcctttaca tgtttagaag
     4501 tacaatcatt gtttgatgat atatttaagg tgaaattatg atgaataata caattggaga
     4561 aaagattaaa caatatagaa aatcgataaa tatgtcaagg gatgagctag ccaacaagat
     4621 tggttgttca gttcatgcta tagctaaata tgagcaaaat caaagaatgc ctagtttaga
     4681 tatgataaga aaaatagcta ctgctttatc aattgctata aataaactca ttgttgaaga
     4741 aaatgattta gatactttaa caaataataa tattaattta aaagtatttg ataaagagtt
     4801 ggatttagta attcacatat tagaaaatta tggttatgat ataaaaacag actataaaaa
     4861 aaagtttgaa attacagata atgcagatac aataaaacca attaacctaa acttctctga
     4921 cttcaaagac ttctccaaaa atctaaattg ggtgataaat ggtcttgttg aaaattttat
     4981 acaagaaaac tctaatggtc ttattgaaga tgaggatgaa gaatattagt attgcacatt
     5041 ttcaaaatat gagtttacct attaactttt atacattcat agttaataaa taaaaattag
     5101 atatgaaagg agctaaaatc atgaataaat catatatacc aaaatacaca cataaattcc
     5161 ctactaatct acctcatgga gataaaaaat atactttaac atacattaga agaatgataa
     5221 gtgaatttgt atatgatatg ggtaacctag aaaataaccc ctttacattc ccagaagtac
     5281 aaacattact tgatggtata accgtaggcg gacataaatt aagtgaccaa aatcaaatac
     5341 taaatataaa atcatcatgg gattatgtaa taaaattaag taacaaagaa aacttatctt
     5401 taaataaaga tacaatttgc tctatacata aaatagttgc aaaagatgaa gctctaatcg
     5461 ttggagattt cagaaatggt aatataggta tagctggtac tactcaatat gaatgcatag
     5521 aagccactct attaaatgat ttatttgtaa atgacataaa tatcataaat aaaataacta
     5581 accccttaga aaaggctata ataataaatt tatggttatc ctattgccaa ttcttctatg
     5641 atggcaacaa aagaactgct agacttacct caaacctaat acttataagt aatgatatag
     5701 gagttctatc aataccagct aaacataaac aagaatacaa cacattaatg ctaaactttt
     5761 atgaaacact agaagctgat gaagttataa aattcttatt agagaaatgt attacattct
     5821 tcgatggata taactataaa tcatataagg agttgtttaa aaatggcaac taaaagagct
     5881 aatggagaag gctctatcgg aaaatataaa gatggttggc gttctcgtat aatgattggt
     5941 tataatgaag atggaaaacc aataagaaaa gaattttatg gtaaaactca aaaggaagtt
     6001 aaagataaac ttgatgctta taaaaaacaa tattatctaa gttctgatat ttcagatgat
     6061 aaaataactt tagaacattg gttttacaat tggttatttg aatatagaat aaaagattta
     6121 aaaccaaagt catttgaaag atatgaaagc atctatcgta attatattaa agatactgat
     6181 ttaggcaatg ttaagttaaa ggacttaaga gtatctcaca tacaaaaata ttacaataga
     6241 ttgcttgatt ccaataaaag tatacctact ataaggcaaa taaacactaa actgaaaacc
     6301 tgcttatcag aagctgaaaa acaaggtctt atacaacgta attattgtac tatggtaaat
     6361 ataccagttg agaaaaagga aaataagctt gaaatattga ctcttgaaca acaaaagagt
     6421 tttataaaag ctatagatgg tcataaatta gaggtattat ttttacttgc tcttggtact
     6481 ggacttagat taggagaatt attaggactt aaatggtttg atattgattt taaaaagtct
     6541 aatttaacag ttaaaagaac tcttcaaaga acatatttta tagataaaac tggaaataga
     6601 gagcttaaag tattagagca agagccaaag acatcaaatt cttacagaac tgtacctatt
     6661 ccaaaagatg ttttaaataa attgaaagag cataaagtaa atcaaaattc ggatatatta
     6721 aaggctggag aattatatag aaatgataat tatgtatttt gcaatgaatt aggtatccct
     6781 atagatgata aaagaccttt aaggaatcta aagtctatat taaacagttt agatatagag
     6841 cctataaagt ttcatggtct tagaaaaact tatgctacta gactttttga gaatgatgtt
     6901 cctcctaaaa ctgtacaagt tttaatgggc cattatgata tatcaataac tttaaatata
     6961 tatactcaag ttatggaaga taagaaggtt gaagctgtag aaaagttgga taaaatattt
     7021 tcattgtaaa aatcaaatac atataaatat tgtaatttca tatagttaat tacaaaatca
     7081 aaataaggat acaaattaat tttatattat cttgtatcct tatttcgtat ctagttttaa
     7141 ttatttatct tggatagcct ttatgataca ttaatccatt ttctaacatt aattcttgag
     7201 ccaagtatat atgaccctta cagtgcatat ctgccacacc ttcacttata agtttatatg
     7261 tttcagaagt atataaataa tccatacttt cttccaagct cttacctgtt tgcttagaaa
     7321 attctaatat gattttttta tatagtattt gtaataatgt tttatttgct tccataatca
     7381 ctatacctcc tcttcatacc taaactttaa atatttatct attacatttt gattggttat
     7441 acatatttgt tcatttggtt tatagtattt taatcttttt aaagcttcat ctttttcaat
     7501 aagattatcc tgatacagtt ctattgtatt gtaaatctta tcatcagcaa taccacctat
     7561 tataatgtca tatttcatat atatatcact catatttcta cattctatta taaaatcaag
     7621 ccattctaaa ttataactat tgaagatttt taccttgcag ttttctttta atttatcaat
     7681 atctaaagaa taactattta aatatgcttt tttacctctt tttttaaaac gctctgtcca
     7741 gttaattgct tgtgtcttta tactggttac atagaatcct tttccaaaat ctaaagaatc
     7801 tcttgaaaag gtaatactag gagtctttac cacacaatat gaaccatgat atacaatcat
     7861 tatatcacac ccttttcttt aagtacttgc attaaatctt ccactatgta acctttactt
     7921 tgagaatgta gtgcttcata gcaaggtatt atatagttat ctaaaatatc agtatcatgt
     7981 ttaagaagtt tataaactcc ttctgaatct atattcaatt tttctgctaa agcttcaatg
     8041 caaaaaatag aaaattctag ttttctttca tccattacaa acctcaactc ctatcaagct
     8101 tatataatat atatttttac taattaacaa cttaaactat cttacattta taaaaatatt
     8161 atatcataaa gtagatatta aaattctatg ttacctatta tcataataaa caaataaaat
     8221 aataaacaaa taaaaacttt ttatacaacc taaatgcacc ccaatatacc cccaaaaaaa
     8281 cctcttttcc tgtggttggg gtacggttgg tggattttat ctccaaaata aaaaatacta
     8341 gtcaacaaag actagtattt ccaagcattt catcattctt tttttaaaat tggtggagac
     8401 gaggggagtc gaacccctgt ccgcaaatct tttccttgct gcatctacat gcttagccaa
     8461 cttcactaat atctcacctc acatagcgcc aagtggcagg ctctatgctt agccagtccc
     8521 aattaatcta cttcaagtcg agacacccct aaagtagttc cctgcataat gacgtccagt
     8581 cttagtcggc aggaggacta agagggacga gcagcaacta ggctgctatc gcgaaattat
     8641 cgtctgcgtt tattttaaag ttccccagtt tttacgtgac ccagagtaac acgacatgca
     8701 gcctcaagtt ccaaacccac gtcgaaacct ttacgccccc ttgagtagtt tatagtccag
     8761 tagacttttc gcaaacttta tcaccatttt tattaaattt ttaatgtata tataatataa
     8821 tacttttaag aataaaaatc aatgttatgt tctagataat aaagaatttt attctaaaaa
     8881 atacagtttt atctagttta atacttacct gtcatttctc tttctatacg tctttgtgca
     8941 tctttcttag ccatatcttg acgtttatca tatagttttt taccttttgc taaggcaatc
     9001 tctactttaa ctttaccgtt ttttagatac aaactaagtg gtataagtga ataccccttt
     9061 atagtagttg ctccaataag ttttcttatt tcatgcttat gaagtaataa ttttctaact
     9121 cttaaagaat caacattgaa tatatttcct tgctcatatg gacttatatg aacttgtttt
     9181 aaaaatactt cgctattatc tacagatgca aatccatcac ttaggttaac tttgccttgt
     9241 cttattgatt ttacttctgt tccttttaat tcaattccac attcataagt ttcttctata
     9301 aaatattcat gtcttgctct tttgtttgtt gctaatattt ttttaccagc catataaatc
     9361 tcaccttctt tgctagaaat ttcttccata aatgttagaa atttactaaa tatttaatgt
     9421 tgccaaatta tttgactaac ttgtatgata acatatttag gcattttagt caatatttca
     9481 ctgatatatt aagttagtta ggctatatat aattaagtaa tataaatgta tgaaaatagc
     9541 taaaaaacta cttcgaatat acttaatact aaactaactc taaccagtaa tactaattta
     9601 taaatttcaa aacaaaaata ttacatatta ta
//
LOCUS       DAEOSP010000147         9425 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.149, whole genome shotgun sequence.
ACCESSION   DAEOSP010000147 DAEOSP010000000
VERSION     DAEOSP010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9425
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.149"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(145..798)
                     /locus_tag="KRH54_002723"
     CDS             complement(145..798)
                     /locus_tag="KRH54_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8580362.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERVLREHWKTIPIAEMKKEKLL"
     gene            complement(1043..2908)
                     /locus_tag="KRH54_002724"
     CDS             complement(1043..2908)
                     /locus_tag="KRH54_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HBG8580363.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(2913..5333)
                     /locus_tag="KRH54_002725"
     CDS             complement(2913..5333)
                     /locus_tag="KRH54_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBG8580364.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(5347..6789)
                     /gene="glgA"
                     /locus_tag="KRH54_002726"
     CDS             complement(5347..6789)
                     /gene="glgA"
                     /locus_tag="KRH54_002726"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBG8580365.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6818..7948)
                     /gene="glgD"
                     /locus_tag="KRH54_002727"
     CDS             complement(6818..7948)
                     /gene="glgD"
                     /locus_tag="KRH54_002727"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBG8580366.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(7950..9110)
                     /locus_tag="KRH54_002728"
     CDS             complement(7950..9110)
                     /locus_tag="KRH54_002728"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBG8580367.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT         2978 a         1722 c          918 g         3807 t
ORIGIN      
        1 tgatttatat atgttaattt aaatacatta ttctatcgta gtgccttaga gtttttattt
       61 gtatttaaag ttaaatttat tcttgttttt cctttaatat ttaatagtaa aacaacaaaa
      121 ttataaaaaa aataaagaga aactttataa aagcttctct tttttcatct ctgctatagg
      181 tatcgttttc caatgttctc tcaaaactct ttcaacattt tctaaatcat tctccctcat
      241 agattttact acttcaatat gttgattatt gctgtatgtc aacattttat ataactcatc
      301 ttcattatca ctagtatatg actttgacat aaaattatat cttaaagagt tcaatagatt
      361 tactaacacc tgattatctg atttagttat gtatatatcg tgaaattcat tacttaattt
      421 taaatagcta ttgtaatttt tatactttat agagaccgcc atcatttccg ttaattcttc
      481 catttttact aggtctgatt cagttaaatt ttgtaatgct gaagaaccgg ctaacgcatc
      541 caatactcca attacggaat aggtgtctaa tttctccttt tcctgaaatt cttttactac
      601 aaatccctta ttaggtattt tttctagtaa attttcacct gctaacctag ttaatgcttc
      661 tctcgctggt gttctactaa ctccgataga tttacaaatc tcagattcag taattctctg
      721 attagcttca tactctccat tttggatttt ctttgatata tagtcatata cttgagttgt
      781 aagtggatct accttcataa gacgttcctc catattaaga ttataaaagt taaatctccc
      841 tacaactttg gtagagagtt cagttttatc ttactaattt atataatatc acaattatga
      901 tattttttat acttctttgt aatattttga aatactaaaa ttcttatgat aatttataca
      961 aaaaaagtag aaaaagtttt gataaaacaa aactctttcc acattcacct tattttctat
     1021 ttatttttct aaaaaaagat atttaattta ctctaaaaat tctaaatgat ttagagtcta
     1081 aatctacttt caactcatta ttttcaattt gtatagtact atttgcattt ttaagactat
     1141 actttatagg tatttcctca ataaaactac tctctataca taatgatata ctttccacat
     1201 tttcacttct atttactaaa attaacattc tatcattatt ttcattaact ctaataaaag
     1261 aaaatacaga atcattatta gtatatataa attctgtatt tccagaactt aaaagtttat
     1321 tcttatttct aatttttata acatctctat aaaaattaag catatcttcg tcttcatttt
     1381 tccatggata agttcttcta ttatctgggt caacacctcc acaaagacct gcttcatctc
     1441 cataatatat atatggtact ccttcaaatg tcatctgtat agctacagca agcttcacta
     1501 aatctacatc atcatttaat tccgttttag ctcttgtaac atcatgtgtt ccaattaaat
     1561 ttaaattaga cctaaatgat tcttttggat aattttcttt aatctgcata tatctattac
     1621 aaagttcata tgaattaatt tctcctttta agaaagaaaa catgttattt ctaaaaggat
     1681 aacccataac agaatctaat tcttcaccta ataaataact tcttctctga ccatagctaa
     1741 tcttatttga tgcatcttcc catacttcac ctataagaat tgagtcatca tctgcctttt
     1801 ttaattcctt ttttaactct cttataaact tagttggtaa ttcgtctgca acatctagtc
     1861 tccagccctt aatacccata tttacccatt tatttataac gctatcattg tcatatatta
     1921 tataatccat atagctatgt tctaattcat ttatatttgg caatgtctta acatcccacc
     1981 aacttttata tttttgagga aattcttcaa acatatacca tgaataatat ggagattctt
     2041 tagattgata tgcccctaaa ctattatagt ttccatacat attaaaatat ttactatctg
     2101 ctccagtatg actaaaaact ccatctaata ctattgatat acctttttct tttgctttat
     2161 caataagttc tttaaatgta tcttcatctc caaacattgg gtcaattttt ttatagtcac
     2221 ctgtatcata cttatgatta ctagaggctt caaatatagg gcttagatat agtatactta
     2281 cacctaattt ttttaaatat ccaagtttat tgattatccc tcttaaattt ccaccatgga
     2341 aatcccatct tataacatcc ccttgactat cctttatgta cataggtgta tcttcccaat
     2401 ttccatatat gaaactattt tttttaggat tatcaacctt accactacga tttccattat
     2461 tgaatctatc tacaaatata tgataaagta ttccttcttt gtaccatgtt ggtactttaa
     2521 aatctttgta aacagtgatt tgatatttat taatatcact ataattatat tcacaagaat
     2581 atccattatc tctgtttttt ccataaaata aagtctttat ctgaccatcc atttcaacat
     2641 caactttaaa gtaataaaaa taaacacctg taccatcaaa cttttcaatc ttaccacaaa
     2701 agcactttcc aagtttatca ttatctaatt cttcatttaa agttattgtt ctcatttctt
     2761 cttctctatt tatttctagg gaaatagact tgatattgta tccctcattt actttaacac
     2821 aaatggtcaa ttcctcatca aattttaaag ctccaaacgg agccttaaaa tttttatccc
     2881 aagagttata ttctattaca ctttgcattt aatcacaccc ttttaaacca aatgtctttt
     2941 gcatactcag aaattgttct atcagaagaa aaaaatccag agttagctat attaactaaa
     3001 gacattttat tccatttttc tttatctcta tataacctgt ttatatctgc ttgagcttga
     3061 ccataatttt caaaatctct caatacaaaa tactcatcat tgtaagtagt gagtgaatta
     3121 taaatacttc ttccttcttc gccaagatta ggaataaatc catttattaa atcatctact
     3181 acccttttta tatccatatt agaatggtat aggtcaagtg aagaatatcc accatattta
     3241 ttataattta gtacttgttc tgcactaagt ccaaataaaa acatattttc tttaccaacc
     3301 tgttcacata tctctacatt tgcaccgtca agtgtagcca aagttatagc accattcatc
     3361 ataaacttca tattacttgt tccagatgct tccttagttg tagttgaaat ttgctcacta
     3421 acatttgctg ctggtattat tatttctgct aatgatactc cataattctc taaaaatact
     3481 acttttaact tgtcctttac tctaacatca ttatttatag tacttgcaac agaatttata
     3541 aatttaatta tacatttagc caaataatat cctggtgctg cttttgcgcc aaatataaaa
     3601 gttcttgggt caagattcaa attaggatta tcaagtaatt catgatacat atgaagtaca
     3661 ttaaatatat tcagtaactg tcttttatac gcatgcaatc tcttaacttg aacatcaaat
     3721 atcgaacttg ggtctacatt taaatcgtat ttatctttta taaactttgc tagatttgct
     3781 ttattattgt attttatatc atcaagtctt tgtaaaacag aggaatcatt tttaaatttt
     3841 tcaatatttt ttaattgtaa tgtatctttc ttccatgaat caccaattaa atctgtaatt
     3901 aaattgctaa gttgaggatt tgaacttata agccatcgcc tatgtgcaat tccattagtc
     3961 ttattattaa atttatttgg ttcatcttga tagaaatctt ttaatacctc tgtttcaaga
     4021 atatgagtat gaagcttagc aactccattt acactatgac ttgtgacaat acataagttt
     4081 gccatattga tattgtcaca gtctattata ctcattcttt ttattctatc ttggtcatat
     4141 cctttattat ttaattcctc tacatatctt ctatttattt cttctattat catataaatt
     4201 cttggtaata gttctttcat catacttaca ggccattttt ccattgcttc tgacattata
     4261 gtatgattag tataggacac tgttttttta gttatttccc atgcttcatc ccaagataaa
     4321 ttttcttcat caagcaaaat tctcataagt tctggtatgc ataaggtagg atgtgtatca
     4381 tttatatgaa ttgctacctt atcgaataaa ttattaatat ttaatttgtt ctttttatat
     4441 tttcttatta tatcttgtat ccctgcactt acaaaaaagt attcttgttt caacctcaac
     4501 aacttacctg catagtttga atcatcagga tataatacct gtgatatttc ctcagtataa
     4561 tacttatgtt ttaaagcctc ttcatagcta ccagaataat ttagagcatt tgaggtaagt
     4621 tcaccaaaat ctctctttgg tatttcactc ttaaacaatc ttaaagtatt tatacattgg
     4681 ttttggtatc cgattattgg tatatcataa ggcattgcca ttacaggaag gtagttttta
     4741 tgaattactt ttaaatgact accttccttg attaattcta cctcaccacc aaacttcacc
     4801 attgttgctt catttggtct tacagtttcc catgcatatc gtccttctgt aagccagtta
     4861 tcaggcactt ctacctgata accatttaca aatttttgtt caaacagacc atatttatac
     4921 cttattccat atccttgacc acttatgttc aaagatgcca tagaatctaa aaagcaagct
     4981 gctaatctac caagtccacc atttcctaag gctgggtcta cttcagcttc aattaaatca
     5041 tccaagttga ttcctaattc agataaacct tctctaactt cttcttcaat atttaaattt
     5101 aataaattag attttaactg tctccctatt aaaaattcta aactaaaata ataaacttct
     5161 tttttcttat ctaactttgt agcaacccat ttttttgcta tctcatcttt caatagacta
     5221 cataatacat ttaacaattg ttcatcagta gcttccttga tagattgtgc atataagcta
     5281 tacattttta cttcaaattt ttgcttgaat ttctttttgc ttattgttac caaaaaaacc
     5341 actcctttaa tcacatatgt ctctatatgt ttctatatac tccttagctg attttttcca
     5401 actattatct gttttcattg cattttcaac taatttaatc catttttctt tgtccttaaa
     5461 tactttgatt gcatttttca aacagaaaaa catctcatga gcattgtaat ttttaaaact
     5521 aaatccattg ccttctccag taaatttatt ataaggtgtt actgtatctc taagccctcc
     5581 agtctctcta actattggta aagaaccata tctcatagct aacatttgac ctataccaca
     5641 aggctcaaat aatgatggca ttaagaacat atcacttgca gcatatatct gttgagccaa
     5701 tgatgcatta aaagtaattc tagctgatac tttatctgaa aaattagaat cgtaatagtt
     5761 gaacattgat tgatattgat gttctccagt tcctaaaacg acaatctgta aatcttctct
     5821 cataagttct ggcatcatgt atgctattaa atcaaatcct ttttgagaaa ctaatctcga
     5881 aactatacct atcaacggaa tagatgaatc ttttttaagt cccaatatat cttgtaatct
     5941 caatttattt tctacttttt tgtcaattga gtctacatca taatgaacaa atatatcttt
     6001 atcttttgca gggtcattta aatcataatc tataccattt aatatacctt ttaattttcc
     6061 tgactctttt ctaagtaatc catctaattg ttcaccataa aaagatgttt gtatttcatt
     6121 tgcataagta ggactaacag ttataatttt atctgcaaaa tttataccag ctttcataaa
     6181 atttattgag ccataatact ctatatctcc attatcaaag tatttttgtg gtaaatccaa
     6241 tatatcatat aaaacatgct tatcaaatac accttgatat tggagattat gtatagtata
     6301 catagttttt atatttttat acccctcaag ggttgcatat ctttctttta gaattaatgg
     6361 taacatccct gtatgccaat catttatatg tattacatct ggataaaaat caattctact
     6421 tatagcttct aaaactgcat ctgtaaagaa agtatatctt tctgcatcat ctccatatcc
     6481 atagatactc tttctttcat cttctcttct aaaataaaat tcattatcta taaagtaaaa
     6541 ttttacacca tccaattcca tttctaaaag accacagtat tgatttctcc atccaacacg
     6601 tacactaaac acagctattt cttttagttg atcttttaaa taacttggta tagttgaata
     6661 tttgggcatt attaccctta catctacacc ttcttttttt aattctttag gcaatgcata
     6721 agaaacatct cctaatcctc cagtttttgc aaagggccaa cactctgctg ttacataaaa
     6781 aaccttcatt agtcattccc ccttctaaaa aatttattta tatatttgta ttcttttcca
     6841 ctacaagagg atatttttca tctcctctta attctttccc atttgataca ctcgtatttt
     6901 tatctaatat tgcattatat agttttgcat ttgactttat tacacaattt tgcattacta
     6961 ttgagttctc tataactgtt cctttttcaa catttacctt tctaaatatg atactatctt
     7021 ttacagtacc ttctattaaa caaccagatg ctataaagct attttctact ttagcactat
     7081 ctgtataaat tgtaggtgat tcattctttt cttttgtaaa tatctttctt tcagaatata
     7141 ataattcatc tgcaacttct aaatttaata aatctttgct catttcaaaa tatgattcaa
     7201 cagaatttac acattttaga tatcctgtat attcataggc tcctattttc atgttttctg
     7261 cttcttcaat aataaattct tctaaataac aataattgcc tttatttaca atgttatata
     7321 tacattcaac aaaatcttca cgcttcataa tatatgtctc catacatatg tttgctctag
     7381 ggaacttacc taaattattt cctacattta ttaccctatt gttactatct aaatttagag
     7441 ttaagttacc agcaaaatct ttatttgcat tatttacatt tttatatatt atagttatat
     7501 cattatgaga ttttttatgg tattccaatg cttgagaata atttaaattg catagcatat
     7561 agcttggagc tattaaaata tattcttctt cactcttttc tatataatct agattagcta
     7621 agatagtata tatatcacct tctctatgtg agtaagtatt tctatactta gtgttttctg
     7681 gactaaaaat tgataggcca cctttttttc tacttaaatc ccaatcttta ccactaccta
     7741 tatggtctgt taatgatctg tatttttctt ttgcgaatat tccaacttta gtgattcctg
     7801 aatttatcat attagataaa gcaaagtcta ttattctata tcttccagct attggtgtag
     7861 atgctattgg tcttccatag ttaagtttgt ttattgctgg gtctcctttt ttgtttaaat
     7921 ttattatccc taaacattca tttctcatct taaagacctc cttctattaa tacgcatcta
     7981 ggttcgatat tttcatactc agaaactaca tttatttcat tatttacatt ctttatctga
     8041 acattatcat ttatagttgc acactcacct atcatagctt tttctatagt tacattttca
     8101 cctattacaa catttggcat tattactgaa tcaattactt tacttccttt accaacaaca
     8161 actccatgtg ataatacaga gttacctact tcacctaaaa ttctacaacc atcagctatc
     8221 attgatctat gaacatttgc atttttacct atatattgtg gaggcattgc catagtattt
     8281 gtatatatct tccacttagg gtctgctaaa tcaagtgttt ctggagattt tattatatcc
     8341 atattagcat cccatagact ttgtatagtt cctacatctc tccagtatcc tttaaatgga
     8401 taagcataca ttccaacatt atcttctaac attttaggta ttaaattttt tccaaaatca
     8461 tcataattta tttctggatt tttttctgct tccttaaaat aatttctcaa cattttccaa
     8521 tcaaatatgt atactcccat agatgccaaa ttgctcttag gttcacttgg tttttcttca
     8581 aattcataga tacttgaatc ttcatttgta ttcattatcc caaatcttga agcttcatcc
     8641 caaggaactt caatcactgc tattgtagct ttagagcctt tttccttatg aaattttaac
     8701 attttgctat aatccatctt gtatatatgg tcaccagata atatcaatac atattctgga
     8761 ttataggtat ctacaaagtc catattttga tatatcgcat gtgctgtacc attataccaa
     8821 tttccttctt tttcattcat aaatggttgt aaaacataga caccaccatt tattctgtcc
     8881 aaatcccaat gacttcccat gcctatatgt gaatttaata tcaacggtct atattgagtt
     8941 aaaacaccta cagtatcaat accagagttt gaacaattgc ttaacacaaa atctataatt
     9001 ctatacttac ctccaaatga tacagctggt ttggcaattc tttttgtaaa aacacctaat
     9061 cttgaacctt gccctcctgc caaaatcata gctaacatct cttttttcat ttaatgacac
     9121 tcccctcatc tagtaattaa tatttataat tgcattttta cttttataaa ctttatcagt
     9181 caataaatgt cgtgtataaa tgttgcttaa ataatacctt aataaaaata aaataatatc
     9241 aactatacaa ttgatataat gtatacccca taatggactt accgacgtat acattatttt
     9301 ccataagcac tatagtattt attaatattc aattatcggt taaaaaataa tacaaatttt
     9361 attttataaa tataaaaaca ttttttataa atcttttgtg tcatataaac atgcttattt
     9421 tttaa
//
LOCUS       DAEOSP010000148         9408 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.150, whole genome shotgun sequence.
ACCESSION   DAEOSP010000148 DAEOSP010000000
VERSION     DAEOSP010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9408
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.150"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(168..911)
                     /locus_tag="KRH54_002729"
     CDS             complement(168..911)
                     /locus_tag="KRH54_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBG8580368.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNATSQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            1079..1951
                     /locus_tag="KRH54_002730"
     CDS             1079..1951
                     /locus_tag="KRH54_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG8580369.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            1951..2616
                     /locus_tag="KRH54_002731"
     CDS             1951..2616
                     /locus_tag="KRH54_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBG8580370.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            2637..3494
                     /locus_tag="KRH54_002732"
     CDS             2637..3494
                     /locus_tag="KRH54_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8580371.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(3650..6196)
                     /locus_tag="KRH54_002733"
     CDS             complement(3650..6196)
                     /locus_tag="KRH54_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG8580372.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYISSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(6196..6882)
                     /locus_tag="KRH54_002734"
     CDS             complement(6196..6882)
                     /locus_tag="KRH54_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580373.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(6965..7954)
                     /locus_tag="KRH54_002735"
     CDS             complement(6965..7954)
                     /locus_tag="KRH54_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8580374.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(7941..8627)
                     /locus_tag="KRH54_002736"
     CDS             complement(7941..8627)
                     /locus_tag="KRH54_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8580375.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(9127..>9408)
                     /locus_tag="KRH54_002737"
     CDS             complement(9127..>9408)
                     /locus_tag="KRH54_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580376.1"
                     /translation="KSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGND
                     STRDGFKMRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT         3331 a         1583 c         1390 g         3104 t
ORIGIN      
        1 acttttaata caaaacaatc attctatttt aacatttatc aatatgtcta aatttatata
       61 attaacacat acgaatagta ttgtttaaat cacattattt aagatagata aatcttaacc
      121 ctactccagc ataaatacta aaatagggct tagaattatc tatattttta tcccataaaa
      181 tgcttagctc ttcctcttga ttgaacatat atatctgggg aaatacaata gaagcgtaaa
      241 tttataaaat ttccaaagtc agaaaacttc agttcaaact taggtatacc aaaactttca
      301 ggtgctattt cgtcaacgcc aaaatagatg ataattccat tatctgtaag ataaaatgct
      361 tggtcttcag gtataactaa ctcaacatta gaataataca tttctctatt ttcatttatt
      421 tgagatttaa tgtaatcagt aattatatta atataatcaa ctccatctct aaaaatgtca
      481 ttaagtaaaa gttcttttcc agttgtcaaa tctatattgt aattattcaa tgtattgtat
      541 ttaggcccat agttattagc aaaccccata aggtttaata tcatactcag tacataattt
      601 ttattaaaag taactgcgta actggtagat acccaatagc tttgttttgg aagtgaattt
      661 tgagaagttg cgttattata ttctcgttct tcttcctgta agccattttt aaaattattt
      721 acagtcattc ttacagcatt gtttatgtta tttaaaataa ctgggtttac atacgtggat
      781 tgatttctaa tattgtatat aacaggatac tttacatcat atgaaaaata cttacttctt
      841 tcaaaatctt cattaaattg tacatataat atattatatg tacaggtatt tgtagattga
      901 taattttcca ttaaaatcct ccccctttta taaaaaaatc agatacaata taatatgctt
      961 aaaacagtaa taatgttaat ggtaaatgta aagaatagaa agtatctaaa acagcaaaaa
     1021 atgactaatt ttgtggtata atacatagga ttgagagtta ttttatagga ggaaaaatat
     1081 gttattatta gcagataaat ggaaagatta tgaacttata gatatgggaa atggagaaaa
     1141 attagaacgt tggggaagtt atgtacttag aagaccagac ccacaggttg tatggccaat
     1201 ggaatctgaa tgggctttat ggaaaaaccc tcatggacat tatcacagaa gcaataaagg
     1261 tggaggtcaa tgggaacaca aaaaaagata tccagaacaa tggactataa attataaaaa
     1321 cttaaagttt cacattaaac caactggctt taaacatact ggattattcc cagagcaagc
     1381 agctaactgg gattggatga tagagaaaat aaagaaagct aacagaccta taaaagtgtt
     1441 gaatcttttt gcatatacag gtggagctac tgtagcatgt gcatctgctg gtgcagaagt
     1501 atgtcatgtt gatgctgcaa aaggtatggt taattgggca aaagaaaata tagaattatc
     1561 aggtcttaaa aatcaaacag taagatttat agtggatgat gttgtaaagt ttgttgaaag
     1621 agaaataaga agaggtaaaa aatatgatgc aataataatg gaccctcctt cttatggaag
     1681 aggtcctaaa ggagaagtat ggcaaataga agaaaagtta tacagttttg tagatttatg
     1741 tatgggagtt ttatcagata accctttatt tttcttaatt aattcttata caacaggatt
     1801 ttctccaata gttcttgaaa atgtattgtc tacaacaata ggtaaaaaga caaatacagg
     1861 agatatatac ggtggggaat taggaatacc agcttccaga gatggtaaga tacttccttg
     1921 tggtatattt ggaagatggg agaaaaaata atgataaaag tcatatatga agataaccat
     1981 ttattagttg tagaaaaacc agttaacata ttgtctcaag gagataatac taatgataaa
     2041 gatatggtaa atcttttaaa agactatatt aaaaaaaagt ataacaaacc tggaaatgta
     2101 tatattggtt taatacatag attggataga ccagtaggtg gagttatggt gtttgcaaaa
     2161 acatcaaaag cagcatctag attgtctgag caggtaagaa gtaagacttt caaaaaaact
     2221 tatctagctg ttgttcatgg caagataaaa tctaactcag atatactaaa agatttttta
     2281 tataaaaata aaaaaactaa tatggtaact gtagtgaaaa aaagccataa agatgccaaa
     2341 gaagctgaac ttaactataa tgtattagat tttaaagaaa atctaagttt agttcaagtt
     2401 aatttaaaga ctggtcgttc tcatcaaata agagttcaat ttgcaagcag aaaacatcca
     2461 ttatacggag accaaagata tggcaaagaa ataaataaga ttggacaaca aatatctctt
     2521 tggtccaata agatagaaat ttgccatcct actacaaaag aaaaaatgga atttacatgt
     2581 catccacctg acaaatatcc atggaatata ttttaaatct caaatgaggt gattatatga
     2641 gtaaaaaatt ttcaatagga gaaatgtcta aacttcataa aataagcata cagacactaa
     2701 ggtactatga ccaaatagat ttatttaaac ctgttcatgt tgaccaagaa agcaattaca
     2761 gatattactc aattgaccaa ttttcacaat tagatattat aaaatattta aaatatttag
     2821 gtatgccttt aaaagaaatt aagtcaaagc ttgatgaaaa taatgaagaa attctaaaat
     2881 tactaacaaa taaaattaat atagtggatg aaaaaattaa agaattagag cttataaaga
     2941 aggtactaag cacaaagaaa gaaactatgg aaaaaggtat gaaagtagat gaagttggta
     3001 aaatagtgag aaaacatatt ccacgaagaa gtatcttatc tatagagtat gaagaaggat
     3061 taagtagtga agaaactatg gaactttcca gaagacaaat agcaaatata ttagaagata
     3121 atatatctgt gttctatggt ggagtaagtg gacttgtatc tatagataaa tttttaaaag
     3181 aagataaagt tatttatgac aatagttttg ttactgttga aagagactta tttgatataa
     3241 aatcacaaaa acatataaca gaaatttcat ctggtgattt tatttgtatg acatataaag
     3301 ggccttacaa agaaaattat accgctttta agaaactggt tgattatgtt gaatataaaa
     3361 gaatacctat tgaaaattat atatatgaaa tacctataat agaccctctg tctacaaaca
     3421 acgaagagga acttctaaca gaaattcaaa tacaagttaa aaagagagaa attgacttaa
     3481 gatgtctagt gtagtaatct agtttactta cacatcttaa tatagttgtc tattatcaac
     3541 atatgatttt tctagatagt gattcctaac agtgagattt tcaaaaaata ggaggaaata
     3601 tggctaatgc taattccctc ctatttttaa ttaagccata tcattttaat taagccatgt
     3661 catcacgaag tgcatcaata atattttctt tttttatctt attaatagta tacaacattg
     3721 taataaatac tataaataaa acactaaata tactaattcc aatactaaac catggaatca
     3781 caaaatcaat atcatctgta accattactt tatgaattag ccatgaagaa attactgcta
     3841 taggaagtcc aaagaacaat gctctcatgc cataaaaggc acactcaaaa ttcatcatct
     3901 tttggaaatc atgctcagac attcctacag agcgaagcat ggcaagttct ctacgacgta
     3961 acttaatatt agttgaaatt gtattaaaca cattggcaat cgaaatcaga gaaatcataa
     4021 ttatgaaagt ataagcaaaa acattggcaa taaaaatgta attacggttc tcatccatca
     4081 ttccatacat attgtacaga ttgtactcat tcgtaactcc ctcactctca ataatttgct
     4141 ccatttctgc aactgactga gtaggatttt tcgattgaaa gcttaaactt tcaattcctg
     4201 tagaaacacc aggaatctca aacttatctt tggctgaata aggagccaat actctgaagc
     4261 ttgctttctt cacatctgaa cttctgattc ctggaggaga atcaggtggc actactttga
     4321 caaatgaaat attcacattt tgtctctcag gtatctccaa cttgccatta gtttcaggag
     4381 tgaggctaac attcatagaa gaactgttga aaatatcaat aatattatta tcctttgtta
     4441 ccatagaaac agcaatcagt tttgcatttt gtccagtata ttcttctgct ggtaatccaa
     4501 gacttttaat aatattcaga taagttctat catcaagaaa ttgcatatct actggtaatt
     4561 caagcatttc actcatagaa tgtgaaccct catagttcaa ataatcgtct gtaagctcac
     4621 ttactttggc aacacatgaa taggtcataa gtttctgata caaaccttcg taaacaccat
     4681 ttgcagtttt tagcttatcg taaagcttca acatttcgtt gtctttcata ccttgtgtag
     4741 aaaaaataat atcctggtca gtaacctctg acctctgatt tgacatctgt tttaggtctg
     4801 ctactaaagc acttgtagat ataaatagaa ctatgcttaa aacaagcgac aatacaatac
     4861 ttcgataacg tttcttgttt cttttaaagt ttttcatagc aagaactccc tcaagaccat
     4921 aaactcgctg tgttagtttt gatgtcttca cagatttaga ttcaattttt acctcatttg
     4981 tctgacgaat acactccatc actggtgtgt tggcagcctt tcttgctggt atgtaagcag
     5041 aaattaagat tgtaaccata cttaccactg ctgcaacgat gattgcaggg atagacaatg
     5101 ttaatgtcaa cggaacgttt acatagagaa tatttgcaaa gtttttagct atgacagaaa
     5161 tcacaagtcc aatactgact ataccgattg caacaccaat tggtatacca atcgcaccaa
     5221 tgcaaattcc ttcaaatagc accgaatttc gtaactgttt tgctgtagct ccaacagatg
     5281 aaagtattcc aaattgatgt gttcgctcgt tcaatgacat gttaaatgaa ttatatatta
     5341 gaaaaattga gcctagcatg attatggcaa gcacaatgcc accaaccgag tacaaaaaca
     5401 tattgaacaa attgttatca gaaacaccta tgaagcgcaa cacattgtca ttgaaaatgg
     5461 aagcatggtc tttagtgcta cttatgtaag agcgaatctg atgtggattt ttcaatgtga
     5521 caaaaaggct aagactgtca gtcttgactt gtttatcttc tttggttatc aaggtatatc
     5581 ctggtgcaga agattcttca aatacgggct ttctacagat acccacaact gtataagttt
     5641 tcttatcctg tggcacaaaa acttcactcc cagcagtgta tgggtcactc tgaccaagct
     5701 tttcattttc actcattcgt ttccctacat ccaaagaaat agtatcacct actgcaaatt
     5761 tgacaccacc atctgtcgca actttacttg aaacaagaat ctctctacta ttttcaggta
     5821 atcttccaga aattaaagtt ataggcaaag tatcaaaagc ttctttgcta aatccagcaa
     5881 taaaaagata tggtttatct ggatttctgt taccttcaag tgttgcatag ccaatatttt
     5941 caaatgttgc agtatttgca acttcatcat tgactctttg ttcctgtaca aaagaagaat
     6001 ccacatccaa aaactggaca tgccaatcac catattttat aatagctcca tttgtcatat
     6061 agttcatcaa agaaactcca aaggtagtaa ctgctgtaat catagcagct gacagaacaa
     6121 ctcctataat tgttacaaga gttcgagtgc gattcttttt caatccttgc agagcaactt
     6181 tgttaaaaat gttcattaac gattcacctt cctctcatca cgcactactt ttccatctga
     6241 aatacagata attctatctg cctgtaaagc aatgttttca tcatgtgtga caataatgag
     6301 agtttggtga tatttactat ggctctcttt cagtaactgg ataatctctt gtccattttt
     6361 actgtctaag ctacctgtag gctcatcagc aagcataact gctggtgcat tcatcaatgc
     6421 acgaccaatg gcaacacgtt gctgttggcc accagaaagt tgatttggta aatgtgtttt
     6481 tcgttctttt aaaccaagca attctaacaa gtcatttaac cgttcttgat tgaccttacg
     6541 cttatccata agtataggca atgtgatatt ttctactaca tttaatgtgg gtatcagatt
     6601 gtgaaactga taaatcaatc caacttgtcg cctacgaaaa atagcaagtt tttcattgct
     6661 ttggccatat acatcttgcc cctccagata tatctttcca cttgttggta cgtctactcc
     6721 tgcaatggaa tgtagtagtg tagatttacc agaaccagaa gaaccaatga ttgcagtaaa
     6781 ctctcctttt tcaatagtaa gtgagacatg gtcaagtgca gtaaccttat tttcattctt
     6841 accataaacc ttacataaat cttcaatctt taaaaacgcc attatataag tctcctttca
     6901 tattttataa atatatcata ggactttcaa cttacattta agtgacattt aaatgagaga
     6961 ttcgtcactt tggaaaacga ataacaaata ttgcaccacc ttgtgggtga ttttgtgcag
     7021 taattgttcc tccttgtctc ataataatcg tcttacaaag tgccaatcct attccataac
     7081 cggctgtgct tgaatttttc ccacgataaa atctgttaaa tagacatggt aaatcttctt
     7141 ttttaaagcc tgctccgttg tcatgaatag taagttgagt aaacaaaaag ttgtcctcac
     7201 aaatgatatc aatttttcca ttatcaccca cactctccat gcagttttta aatatatttt
     7261 gaattgcttc tgaaagccaa tttaaatcac ccaaaatcct tactcctttt ggtatatcaa
     7321 tatgtaaagt gatattgtgc agttccatgg gaatcaaaag ttgatgaagt gcagaactaa
     7381 tcaaattatt cacatcaatt tgttcttttt gaaagactac aatacctgca tctaaacgtg
     7441 ataattttaa aagggaagta atcagccagt ccattcgtat aagtaattcc tctgtttctc
     7501 gtatcaactc ttttctctca tcttcatcag aagtattctc taacagtgac aaaataagag
     7561 ttactgatgt aagaggagtg cgaagctggt gggctatgtc agctagtgag tcagcaaggt
     7621 gttctttttc ttttttcaat gcgtaatttt gctctctaat acgtaacgtc attttagtta
     7681 tttcactttg cagaatagaa agttcaccct catctgattc agcaatatac agatggttag
     7741 cattatgaag cacaagatta atttgttctg aaatctgtgc aatacttttg tatcgatttt
     7801 tagtaaacac aaaaaatgct attccaaagg tagtggcaga aacaatagaa agtattccag
     7861 tcataatact gattgcaaat cccagggcta cagtgagaat agtaattaat aagaataaaa
     7921 tagcaaattt tcgaaactct ctatttcgaa acataacttc cccccaatct ataccctgtc
     7981 ccacgaacag tcaaaattat ttgtggactt gaagggtcat cttctatctt ctctcgcaag
     8041 cgtttgatgt acacagtcaa tgtgttgtca ttaacaaact cacctgccgc atcccacaat
     8101 tcatcaagta atcttaccct cgtaataata cttttaggat tgctaataaa caccaataac
     8161 aaacggtatt ctaaagctga aagaaaaacc tcattgccat tctttttcac aacaccggtt
     8221 gccgtatcta cataaagccc acaaatttca aaagttgacc caaaacgccc actttttcgt
     8281 agtgctgttc taattcgcgc aatcagttca cgtggacgaa aaggtttagt aatataatca
     8341 tctgcaccca tattcagtcc agtaacaaca cttgattcat caccggaagc tgtcaaaaag
     8401 ataacaggaa tgtcttgctt ttctttgatt tctgtgcaaa tcgtaaaccc atttccatca
     8461 ggtaaagaaa tatcaataag tgccaaatca aatttattct tagcaatctc tacaagtgct
     8521 tcacttcttg tggatacatg ggttactata aatccttctg aattaagcaa aagaataaga
     8581 tttttggcaa ttgctttatc atcctcaact aaaaatatcc gtttcattta ctttcatcct
     8641 cctttatttt attagtttta tatattccag cagagcaata gtacactact attttactta
     8701 tcattacaca attttcagta gtttcttttg ttcttatata atccatgcat aatacctcca
     8761 ttgatttatt atatttctta taacataaat acacaagata agacaaaaaa cataatgtaa
     8821 gtgcaagttt tttaactcac taacttataa acatacactc ctactaacac tactttcttg
     8881 atgacacatc ttacaagctt atattcatac atcaatgaaa aattaaattt atccatttat
     8941 taaaataaaa ggcaatagct tctatagcta atgcccttta tcttaaaatg atttaaatac
     9001 tatacaattt ttataaatgg ctataaataa actcgtatag aatataaaat ttatctacag
     9061 tcttttgtta tacaaaattt ataacctctt atagaacaaa tttaccactt tgcaaactta
     9121 tcacttttac tcagctatat tataaacttt taatttatct ttgtttaaat gtttcttctt
     9181 aacttctact aaagctttta gcgtatccaa agatgtcatt atttcagtag aaaattcaat
     9241 ggctgttctt ctcatcttaa atccatctct tgtagagtca tttcctttag ttggtgtatt
     9301 tattactata tcaacttgtt tatttcttat agcatctaat atgtttggtc taggctcttc
     9361 aattctattt actatatcag cctctattcc attttctctt aaactctt
//
LOCUS       DAEOSP010000149         9298 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.151, whole genome shotgun sequence.
ACCESSION   DAEOSP010000149 DAEOSP010000000
VERSION     DAEOSP010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9298
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.151"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..695
                     /locus_tag="KRH54_002738"
     CDS             <1..695
                     /locus_tag="KRH54_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG8580377.1"
                     /translation="IVCKNLNKKTIIIVIILVSLAFELTQYIFALGSSDIDDVILNSL
                     GGIIGINIYINMNKLFPSDINRFKAIIGTSLVLGVIGLGVISKNYHNLLTFKFRPDRK
                     ISKILIEENREIIKDINKDTVDIVGTFESFKKGIITIKAGSNNKVKRPENLIDSDGNI
                     RIYLNENTKLVSYIITKESKMDIVKYEEFDVKNLDLLRRYDTINVWIDKENQPKDKHG
                     IMASKLLIGLYE"
     gene            810..1595
                     /locus_tag="KRH54_002739"
     CDS             810..1595
                     /locus_tag="KRH54_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580378.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            1887..3206
                     /locus_tag="KRH54_002740"
     CDS             1887..3206
                     /locus_tag="KRH54_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG8580379.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(3281..3709)
                     /locus_tag="KRH54_002741"
     CDS             complement(3281..3709)
                     /locus_tag="KRH54_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580380.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(3800..5176)
                     /gene="hydG"
                     /locus_tag="KRH54_002742"
     CDS             complement(3800..5176)
                     /gene="hydG"
                     /locus_tag="KRH54_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBG8580381.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(5177..6262)
                     /gene="hydE"
                     /locus_tag="KRH54_002743"
     CDS             complement(5177..6262)
                     /gene="hydE"
                     /locus_tag="KRH54_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBG8580382.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(6298..7506)
                     /gene="hydF"
                     /locus_tag="KRH54_002744"
     CDS             complement(6298..7506)
                     /gene="hydF"
                     /locus_tag="KRH54_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBG8580383.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(7521..7772)
                     /locus_tag="KRH54_002745"
     CDS             complement(7521..7772)
                     /locus_tag="KRH54_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBG8580384.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(8153..9193)
                     /locus_tag="KRH54_002746"
     CDS             complement(8153..9193)
                     /locus_tag="KRH54_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBG8580385.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT         3300 a         1308 c         1318 g         3372 t
ORIGIN      
        1 ctatagtatg taaaaatcta aataaaaaaa ctataattat agtaattata cttgttagcc
       61 tagcatttga attaacacaa tatatttttg ctcttggaag tagtgatata gatgatgtta
      121 ttttaaattc attaggtggc ataattggaa ttaatattta cataaacatg aataaattat
      181 ttcccagtga tattaacaga tttaaggcta taataggtac tagtttagtt ttaggagtta
      241 taggattagg agttatttct aaaaattatc ataacttact aacttttaaa tttagacctg
      301 atagaaaaat atcaaaaata ttaattgaag aaaataggga aataattaaa gatataaata
      361 aagatacagt agatatagtt gggacatttg agagctttaa aaaaggaata ataactatta
      421 aagcaggttc taacaacaaa gtaaagagac cagagaattt aattgattct gatggaaata
      481 taagaattta tttaaatgaa aatacaaaat tagtatctta tataattacc aaagaatcaa
      541 aaatggatat agttaaatat gaggaatttg atgtgaagaa tttagattta ttaaggagat
      601 atgatactat taatgtttgg attgataaag aaaatcagcc aaaggataaa catggtatta
      661 tggctagtaa attattaatt ggattgtatg aataaaatat taaacaagaa taaaagaata
      721 acaaatataa aaaacgaaaa aactatttct ataattattt ttacgttgtg cataaaataa
      781 gaacattagt gaaggagtag agataaatta tggagataat aaaatatatt ttgtactttt
      841 ttatatattc atttttagga tggacagtag aaagtatagg atgttctata gcaagcaaaa
      901 gaataatcaa cagaggtttt ttaaatggtc caatatgccc tgtttatggt tttggagcgg
      961 taattgtgat atcactattg ggaagattta ataatgtagt aatagtattt ctattaggaa
     1021 tgattttaac tacgatacta gaatatttta caggatttat tttagaaacc ttatttcatg
     1081 ctaaatggtg ggattattct gacagaaaat tcaatataaa gggtagagta tgtttaaaaa
     1141 atgcaatata ctttggtgta atgtctgttt taataataag atttatacat ccatttataa
     1201 aatattttgt aagtataatt ccatatagaa tattaatttc aatggctata attacaacac
     1261 tttggactat acttgatttg attgttacta ttataacact taaaaaatta gatataaagt
     1321 taaacttatt agacgatata attacagacc ttaatgatat aaatgtgaaa ttggataaat
     1381 ttgatagagg agaaatacaa gctttattta aaacaataaa taacgacgaa ttagaagtta
     1441 gagaaaaaat aaacaaaata aatagtaagt tggaccgtat agagtctaat attattttac
     1501 aaaaaagagt tataaaagct tttccacata taaaacataa aaaacaacaa gagcaactag
     1561 aacattttaa aaaaattatt aatgagaaaa ggtaattata caatttaatg aagacgaata
     1621 gaataatggt atttataaga tgattttcag aggactctat aaaaatagaa tagagtcctt
     1681 tattttttga aaaataatgt ttatttgata aaaataaaat atattgtttc tgaataacaa
     1741 atattttgaa aattataaaa acatatattg acaatttgtg tcaaaaatac taacattaag
     1801 tatataaaga attttatatg agaatatctt tataaatcgc aataatttta aaacataaat
     1861 ttatttaaag aggggatgat ttgccaatga gcgagataac tagtcaaatg ataagtacag
     1921 aagaaaaaaa atatgttgct aaaactcaaa agatacctta ttatccagta gcatttaaat
     1981 caggtgatgg tgcaatgcta tatgattatg aaggtaatga atatgtagat tttttagcaa
     2041 gtgcaggctc tgcaaatgta ggtcatggta ataaggaaat ctcacaagca gttaaagaac
     2101 aaatggatga tataacacaa tatacacttg catatttcca tagtgatcct cctgtaaaat
     2161 tagctgagaa gcttgtggaa atagcccctg gagataatga taaaaaagta ctatacagtg
     2221 caactggttc tgcttgtata gatgctgcta taaaattagc aagagggtat actggaagaa
     2281 caaagataat atctatgtgt gaatcttatc atggaagtac ttatggagct atatctatat
     2341 ctgctttaag tacaaatatg agaagaaaaa tgggtccatt gttaccagaa gtttatcact
     2401 tccattatcc tgataaaaat agaacagcta aagaatgttt agatgagata gaatatgctt
     2461 ttgcacacta tctaccagca gaagaggtag ctgcaatatt tatagagcca attgcaggag
     2521 atgcaggaat aatagtgcct ccagtagagt gggtacaagg tttaagtaaa atatgtaaag
     2581 aaaatggaat attacttgta agtgatgaaa tacaacaagg tatgggaaga acaggtaagt
     2641 ggtttggtat agaaaatttt ggtgttgaag cagaccttat agttcttgga aaatctgttg
     2701 gaggtggatt acctctagga gcagttgttg gaagaacaga aataatgcaa agtcttgatg
     2761 caccagcaca tttatttact ttagctggaa acacaacagt atgtgtggct gctttaaaat
     2821 ctatagagat aattgaaaaa gaaaacttac ttcaaaagag tatagaaatg ggagattata
     2881 ttaaagcagg atttgaaaaa ctaaaagaaa aatacgatat aattggagaa ataagaggca
     2941 taggattatc tataggagtt gatatagtta aaggaaaagg ttctaatgaa aaacatccag
     3001 atgctacagc taagatatgt tatagatgta tacaaactgg attaataatg atattcttag
     3061 gacaatctac tttaagagtg caaccacctc tagtaataac taaggagcaa gtagataaag
     3121 caatgaacat catagattct gcaatagatg attatttaaa tggtagaatt ggtgatgaag
     3181 tatatgaagt aactcaaggt tggtaaaata aatattaaat gaaaatagga gttatctcaa
     3241 ttttagaaaa ttatttctga aatgagataa ctcttttaag ttacccacaa ataagaaaat
     3301 tgtttgattc attaaagact atatttcgga tgtcgttttt gtgagtatca gagacagtat
     3361 tacgagaata attaccttta ttcgtactat ctgttaattt agttataatt aagatagcta
     3421 aaagaataag aattactgtg tataaagcaa taacatatgg ttttctttta tatttgtatt
     3481 tgttattgaa aaaagaatgg ccataattat ttttatttac tttagatgta tttgtattct
     3541 ttttcttgtt acctttcttt ttatcatcct tttttctatt tttagggtaa ggaatgactt
     3601 tgtgtgtatt atcaagtttg atatttttat ttttttgaat attgttttcc aggctagcct
     3661 ttttttctcc aaattgaata gttttattgg tattgttgtt atctttcaat gttaatctcc
     3721 ctcccttaaa ctttcccttt agataatatt actactaacc tatggaatat acaataaata
     3781 atatataagt aataaaattt tataggtata aatctctttc tccacgctct aaggcagtca
     3841 tattgttaga tactatgttt cttatatctt ctctttctat cttacctatt tcttctgcga
     3901 taagtttttt acccataatt ttaagttctt catctccata atcgattaaa aattcattta
     3961 atgtagtaag agcatttggt gtacatacat tatggatttg tcgactttta gctaagctca
     4021 tgaatctatc gccagttcta cctttacgat aacaagctgt acaataacta ggtatatatc
     4081 catcagtaat cagttcttta agcacctcaa cagggcttct atggtctcca acttcgaatt
     4141 gttctgtatt gtcaccatct tcatatgctt tatatcctcc aacacctgtt agagaacctg
     4201 cactaatttg tgatacacca tattttagta actcatttct catttcagca gtttctctag
     4261 tggacattat gattccagta aaaggaacag ctaatctagt tatagctact atttttttga
     4321 acatatcgtc agaaactaag tgaggaaaat cttctaagct cataccttca gcttttttca
     4381 atctagggaa agatatagta tggaaaccta caccaaattt ttcctctaag tgttcgttgt
     4441 gcatcataag tcctagaact tcaaatttag ggtcagataa accaaataaa actcctgcac
     4501 caacatcatc aacaccagct tccatagctc tatcaaaagc tgttaaatgg taataatagt
     4561 cattttttat agattgacca tgcattttta taaatgttgg tttgtgataa gtttcttgga
     4621 ataatatata agttcctatt cctttttctt ttagtaattt atactcatct acacttgtag
     4681 ctgctatgtt aacgtttacc cttcttatat ttccgttttc attatatgtt gaataaattg
     4741 catctaagca atctaagatg taatttatat cacaattttt agggtcttca ccagcttcta
     4801 gagcaagtct tttatgaccc attttttcta atattttaac ctcttctttt atctcttcca
     4861 tggtaagttt ttttctttta aatttgttgc atttactaaa accgcaataa acacattcat
     4921 ttacacagta gtttgataca taaagagggg caaataaaac cactctatta ccatatattt
     4981 cattttttat ttgcccagct atactaaata atctatctag ttgtttttta tcttccactt
     5041 caagaagtgt agctatatct ttataactaa gtttttccct tctatcagcc ttatcaagta
     5101 ccttttcaat atcatcagaa gttgaatttt ttgcatcttc taataaagaa ttaataagtt
     5161 catggtttat gaacatttac aaacgcctcc attcaatatt atcaccgcga gaatagtcaa
     5221 cttcaagtcc taatgacttg atgttttctt ctaaactttg gtgatattca caagcttctt
     5281 gaccagtaaa ggctttgtta tcgtaaagag aatattgttt tctaaattct tgaggagaaa
     5341 ggtttggcat aattacattg caaccagcta aaagacctgc atttctacca ctagggttta
     5401 tagatgccaa ggctgttgta gcgggtaata aaacttttgg aagtagaagt cttgtaattg
     5461 aaagcataag taatgtcttc tctaaatctc catgtttata atccttaaaa attgtatcat
     5521 ggtgtggaat gaaaggtcct attccaacca tatgaggatt taactctttt aggtataata
     5581 aatcacgaac aaggtcttcg ttgttctgaa atggtgattc aaccataaat ccagcaccta
     5641 cttgataacc aatttcttta aggtttttta ggcattcttt tctagttcta agctcaatgt
     5701 ttggcggatg taatttttta taatgactgt ctgtagcagt ttcatgtctt aaaagatatc
     5761 tatctgcacc acataaaaag tattttttat acgaatcata actcttttct cctagtgata
     5821 gagttatggc acaatcctca tatttatttt ttatacttga aacaatttca catattttat
     5881 catcagtaaa atatgcatct tcaccacctt gcaaaacgaa tgttctgtat cctattttat
     5941 aacctatatc acaacattca agtatttcat caagtgaaag cctgtatctt atgatatttt
     6001 tattagaaga ttgtattcca caatagtagc aattattttt acaatagttt gtaagctcta
     6061 taagtcctct caagtagact ctatttccat aatgtttatt tctcatgtcg ctagcttgtt
     6121 tataaagata gtttcttaaa ttttgttcga agttatattc ctcattgtcg atattttcta
     6181 ttaaatatag tagttcatca aaacttaatg aattaatgct gtaaagttta tctataaagt
     6241 atttaatctt atttttagtc atattcaaat atgtaattat taagtatagt taaattttta
     6301 ataattgaaa agttcaagtg atctttccaa aatacctttt acatatgcta ttaagacacc
     6361 atagtttaca ataggtacat tgaaagattt tgccttttct attctagata acattccagc
     6421 tctattcatc atacaagctc cacagtgaac tacaagggca tatttattta tatcttcagt
     6481 gaaggatact ccagaactaa attcaaaagt aatgtttttg ccagttttct ttctaatcat
     6541 attaggaatt tttactgttc caatatcatc agtttgtcta tgatgggtac atccttcggc
     6601 catcaagatt ttatcaccat cttttaaatt ttctaaagca taagctccat taatcaattc
     6661 tttaaggtct cccttttgtc tagcaaacaa tatagaaaaa gatgtaagag gaatatcttt
     6721 tggtgtatct ttatctactt gtggaaatac ttgagagtca gtaataacaa gttttggttt
     6781 tttgcctaaa ttagatagag tttcttttaa actatcttct tttgtaacta tagaaattgc
     6841 accattatcc aaaatatctc ttataacttg ctgttgagga agtataagtc taccttttgg
     6901 agctgcttta tctattggaa caactaaaac aactaaatca tttggttcaa ttaaatctga
     6961 taccaatttg aactcagtgc tatccttagg caaaactttt attatttcat tttttaaatt
     7021 ttcaattcca attttatctg ttgatgagac agaaacaact ggacatttaa ctttattttt
     7081 agttaagttt aaaatttctg attgattctt aatagtatca attttgttaa gaattagaat
     7141 atgagggata ttcttatcat taaatttttc aattagtgac aaatcttctt gtgatatacc
     7201 aatttggcaa tccactacta gtaaagctat atctgtcttt tctaaaacat ctagtgattt
     7261 acctattcta agttcaccaa gttcaccaac atcatcaaga ccagcagtat ctataagaac
     7321 acaaggtcct ataggaagga tttccattgg tctaaatact gggtctgtag tagttccagc
     7381 aatatctgag actattgcag cactttgatt tgttatagca tttattatgc tagatttacc
     7441 tgcatttctt ttaccaaaga gacctatatg tactcttacc gattgtggag tagaatttag
     7501 actcatttgt actcaccacc ttaaacttct ttttttgata tactagtttt tacagataca
     7561 tgaggtatgt tgcctaattt accagtcaaa ctattaatag tatccatttc gccaacaact
     7621 gctatacata cgattgagac accttcttct tcgcaaggaa tgcccattct tcctttaata
     7681 atacctttaa aacctgatac aacctcattg aatttaaatt gacattcttt tggctcttcc
     7741 aaaatagcac ttataactgc aacctttttc ataaatatcc tcctaactca gaaaatatgt
     7801 cttaaacaaa tccaaaatca aaaaactttt ctttgtaaag aaaagtttct acaaaagaaa
     7861 agttattaat tacacaagta taatgtgcta tattaaacta tttccttaca gattagataa
     7921 ctcttttctg gtagggttag cttgtaaaaa tttaatttaa caacaagtaa ctgaagaaaa
     7981 tttttataca atttatataa attataataa aaattaacta aagaaaattg aagttactta
     8041 tataatagta gtaaattgaa attaagtcta tagaaaaacc aatttttgta taaaaataaa
     8101 aattaaagtt aattatttgt taagaattga ctttggtaaa atatataatt aattattctt
     8161 taagcatata atttataagt aattttagtg tattttcaat agcttctata tgtgttcttt
     8221 cataatggtg actattgttt gttccaggac caacacatgc tatttgaaca tcttctcccc
     8281 aacgaatagc ttctgaagca tctgaaccat agaaagtaaa tacatcgaca gcgtagtcta
     8341 aattattgtc ttctgcaaca tcgacaagct tgtttcttaa attaaaatca tataatgata
     8401 aattatcttt tgcaactata gttacaccat gttcactaga tgtttgactt ggtccagtag
     8461 gtgctatatc tattgctaca aattcttttg ttttttcagg taaacattta gaaacaccat
     8521 gtccaatttc ttcataatta cttatataaa agttagtagt atgcttaggt gttaaattgt
     8581 tttctttaaa atatctacat atttcaagaa ccattgctac agctgattta ttatctatat
     8641 atctagattt tataaaacca gattctgtag cctcaaatct atggtctagg catacaaaat
     8701 ccccaacact tattcctaaa tctaatacat cttgctttgt aaatactttt tcatcaagtc
     8761 taataatcat tgtttcttca tttctttcag tggcagcttt tgtttgacca tatatatgag
     8821 tagaagcata cttaaataca acacttcctc tgattttttt accttttcta gttataacag
     8881 tacagttttc tccctctata gccgcccaac atccaccacc aactttatgg tatttaagag
     8941 taccatcaga tgatatttct tttactatag ctccaagtgt atccatatgt gcagatattg
     9001 ttatttgatt tgaatcatct tcaccaggaa gagtagctat aagtgcacct ttttttgtaa
     9061 gaatagtgtc aagacctaaa ttttcaaaat cttttttgat ttctaaaact gcattttcag
     9121 tatatccagc tgggcttgga atgtccaaat attttttcat taaatttata gtattagtta
     9181 aatttaaatt catattgttt cctcctaaat aaatatattt atacaaatta taacataaat
     9241 acaaatgttc tgaaaactaa aattaaaaga atagaatcta aacatattta aaaaaaaa
//
LOCUS       DAEOSP010000150         9244 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.152, whole genome shotgun sequence.
ACCESSION   DAEOSP010000150 DAEOSP010000000
VERSION     DAEOSP010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9244
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.152"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            394..1461
                     /locus_tag="KRH54_002747"
     CDS             394..1461
                     /locus_tag="KRH54_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG8580386.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNSAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PSSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            1653..2183
                     /gene="rnmV"
                     /locus_tag="KRH54_002748"
     CDS             1653..2183
                     /gene="rnmV"
                     /locus_tag="KRH54_002748"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBG8580387.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            2354..3223
                     /gene="rsmA"
                     /locus_tag="KRH54_002749"
     CDS             2354..3223
                     /gene="rsmA"
                     /locus_tag="KRH54_002749"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBG8580388.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            3289..4551
                     /locus_tag="KRH54_002750"
     CDS             3289..4551
                     /locus_tag="KRH54_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580389.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEENEESDRDEDSEE
                     EYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSDDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            4702..5817
                     /locus_tag="KRH54_002751"
     CDS             4702..5817
                     /locus_tag="KRH54_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG8580390.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            6003..6899
                     /locus_tag="KRH54_002752"
     CDS             6003..6899
                     /locus_tag="KRH54_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG8580391.1"
                     /translation="METRYEEANKITIQSILWNIVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            6969..7766
                     /locus_tag="KRH54_002753"
     CDS             6969..7766
                     /locus_tag="KRH54_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580392.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(7821..9173)
                     /locus_tag="KRH54_002754"
     CDS             complement(7821..9173)
                     /locus_tag="KRH54_002754"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBG8580393.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
BASE COUNT         3712 a         1012 c         1686 g         2834 t
ORIGIN      
        1 taaattgttt ctggataact taaaattgga agtaagaata tttaactaga gaagaattat
       61 ttaagtttag gttagctcta ggagtagctg attttatagg aacaatagat tttatggatg
      121 atgatataaa catggtagca attagaccag aagatataag ggtagaattt aatagagatt
      181 tcaaagaaat tatagacctc aaactcagac cgtatcgata taaaaactaa agttagatga
      241 tatagtgttg tagataggac catcagctgt tatggcagta ttagtgcttt taacaaatat
      301 aatagtaaaa cttttgtatg aattgataat aaaaaaatta aaaaccaaga cataaaaatg
      361 gcaacaaaaa gaagttgaat aagggaggta aaaatgaaat ttaagaagtt agtctctttg
      421 ggaatattat caacactttt attgagtgta ataactggat gtagtaatgt ttcatcaggt
      481 tcaaacttaa agtctaataa taagacaaaa ggagagttaa cagtttatac agcaattgag
      541 gaagatagta tagatacata cttagctaca tttaaagaga aatatccaga tattaaattg
      601 aatgtactta gattatcagt aggaggtata acagcaagat tattagcaga aaaagataat
      661 cctaaagcag atgtgttatg gggtgttgca gctacaagtt tgataatttt agatgacaaa
      721 gaaatgctag agccatacgc tccgaaaggc tttgataagg taaattctgc atttaaagat
      781 gataagaaag ttccttcttg ggttggaata gatgcttgga tgactggtat aacagtaaat
      841 actaaagaat taaaagctaa aaatatatca gctcctagtt cttatgaaga tttaataaag
      901 ccagaatata aagatcttat atctatgcct agtccagctt cttcaggaac aggatattta
      961 acagtatcag aactcataca aattatgggt gaagaaaaag catggcagta tatggataaa
     1021 ttgcataaaa atattggtgt atacactcag tctggttcag ctccagctat attagcagct
     1081 tcaggagaat atcccattgg tatatctttt ggaaatagag gtataaagtt aaaaggtgaa
     1141 ggttatccag tagaagtagt atttccaaaa gaaggttctg gatgggagat agaatcaaat
     1201 gcacttgtga aaaaagataa tataaaagaa gcatcaaaag tatttttaga ttgggctata
     1261 agtaaagatg caatgaatga gtattctaag aattttgcag ttactacaat tgacacggga
     1321 aatccggtgc cagaaggatt tccaaagaat ccattagaac agatgataga taatgattta
     1381 aaatcagcag caagaaatag agaggctata ctaaataaat ggatagaaaa atatgatgga
     1441 aagacagaaa aagagagcta agccttgcaa taaaatttag tacaaaaagc gcctagaata
     1501 taagttttat atctaggctc ttttgatata tttatttaaa atgcatattt ctatataaaa
     1561 ataaatagtt tagtaccttg attaaattaa ataaaaacct taaaataaag agatgtaaag
     1621 aaaaactaaa aacgaataaa aggtgaattt atatgataaa agaaataata gtagtagagg
     1681 gtagagatga cgttacagca gttaagagag caatagatgc agaacttata actacaggtg
     1741 gatttggatt tccaaaaggt gtaatggagc gtataaaagc tgctaatgaa agacgtggtg
     1801 ttataatatt tacagaccca gattttgcag gtgaaaaaat aagaaaaaaa atagcatcag
     1861 aagttccagg atgtaaacat gcatttctac cgagagagga agctaaaaag gatggagata
     1921 taggtattga gaatgctact cctcaaagta taatagcagc tctgaataaa gttagaactg
     1981 aaaatacaga aaaaagagat gaatttaagc aagttgactt aattagaaat ggtcttatag
     2041 gtaatgaaga agcatcactt agaagagatg cacttggaaa aatacttgga attggatatg
     2101 gaaatgcaaa acaattttta aatagattga ataattatgg agttgaaaga gaagaattta
     2161 tagatgcatt aaaacaaatc taaacgtaat gtttaatgta tgaaaaatat gtatataaaa
     2221 taaattgtgg aattacggtt aatttaagat gaattattta taatatacac cttattttaa
     2281 atcaatgtaa atacatataa ataagtataa ttaataaaat taaatatcaa aataagttaa
     2341 aaaggagtaa ataatggata gactatcttc acataatgca acaaaggaag tagtacaaaa
     2401 atataatttt aaattttcaa agtctttagg tcaaaatttc ttgatagata gtaatataat
     2461 tgataaaatc ttatctggag ctagaatcac aagaggagat aatatcatag aagtaggacc
     2521 tggtatagga actcttactc gtgagatggg taaaatagca gaaaaagtag tggctataga
     2581 aatcgataga aatttaattc ctatattgaa agataccttg tcagatttag ataatacaga
     2641 ggttgtaaat caagatatac taaaagtaga catacaggaa ttagtaaaag ataaattaaa
     2701 tggtgggcca gttaagcttg tagcaaatct tccatattat ataactactc caatagttat
     2761 gaaattttta gaggaagaca tacctgtaac agacatagta gtcatggttc aaaaagaagt
     2821 tgcagataga atgaatgcta ttccgggtac taaagattat ggggcattat ctatagctgt
     2881 tcaatactat tgtgatacag agattgtggc aaaagctcca agacatatgt ttataccaca
     2941 accaaatgta gattccacag ttataggtct acatgtaaga gataaaagaa aatatgatgt
     3001 tcataatgaa gacatatttt ttaaaacagt aaaagcatcc tttggacaaa gaagaaaaac
     3061 tcttcttaat tcgttgggag gtcttggttt tcttaataaa gatgaaataa gagaagtatt
     3121 aaaagaagct aatatagacg aaaaaagaag gggtgaaact cttagtatag aagagttttc
     3181 agttctttca aatataataa atacaaaagt atcttctaaa tagaaggtac tttttttaat
     3241 tttgttaaca tatattgttt agagaaactg tgttaagggg ggattgcagt gggttctaaa
     3301 agaaagttta aaagagacta tataatacta gaagctaaag acataaactt tagatacaaa
     3361 gaacgagtac tgccaaaagc ttttgcaaaa gttgaagtaa atgatgaaaa agcaattatt
     3421 gcattatatg tagaaaatct aaaatatgtt aaagatgggt ataaagcagt agcaataaaa
     3481 gatgaccatg gattaataga cttaggaaat ataattttaa atgaacaagg aagaggtgaa
     3541 tttgttttag atttagaaga ttatgatgat gagataaaag gtattgcatt attgtatgaa
     3601 agaaatatac ctttaattgg atttaaagga aataagcttg aaaattatga ggacatatta
     3661 tttcaagagg tagaggaaga agaatatgaa gaatatgaag agttggaaga agaagacgaa
     3721 gagatagagg agaatgagga aagtgataga gacgaagata gtgaagaaga atatattata
     3781 gaagaatatg atgaagatga gtatgaagac tattgggaag atgatgaaga tattgaagaa
     3841 ggagagttaa aagcaagaac ggaagtagaa gattctgatg atgaggaaat agaagagttc
     3901 aataattata aggaaataga aaattataat agggttaaag tacaggaaga atatatagac
     3961 atagaatatg atgaagaaga agaggaagaa gaagtttttg aagtaaatag agaagaagat
     4021 aggtttgttt cctcaaaacc tactaaatca aagaaagttc aaaaagatca gaataatatt
     4081 gaaaaaataa aaagagctaa tggagtaagt caaggagaga gtggaaataa acaaaccagt
     4141 acattactta tgccaagaca aataaaaaaa ggtttgaaat tatttaaaga agtaaaacct
     4201 tttgtaacag attatataga taagacaaga tggtggaaaa tagagataaa tcctacaaca
     4261 ctgtgtggat atacaatgcc ttatttagga tatttaaacg tattgaatta cactatgtat
     4321 agtgatgcag ttttacattc atataaatac agacactatt tatttggagt tcagtatgat
     4381 gaatataata agagaaaaca ttatatttat gctatcccag gaaatagaaa tgaacagcca
     4441 gacaatggaa gcacaggatt tttaagatat caagcttgtg ataatagaac aaatagttta
     4501 ggatattggc tatgttttat agattctaga caaagaaata tagttaggta aaaataataa
     4561 aaatgaaaaa atttagactc aattcttatt ttactgaaaa aattaatgaa atgtagtaca
     4621 aaactttaaa aactattgat aatatttaaa tattacacta caatatatct tgaaatacct
     4681 attatattat ggaggaaaat tatgataaac gagcaaagaa ttttaaatga gtttcttgaa
     4741 ttagtacaga tagatagttt atctttaaaa gaggggaatg ttgctaaagt attagttaaa
     4801 aaattagaag aaataggttg ttcagtagta atagacaatg ctggtgaaaa agcaaatgga
     4861 gaaactggta atataatagc tacattaaaa ggaaataaag aaggaaagaa aatattattt
     4921 agttcacata tggatacagt aactccaggt ataggggtaa aacctatagt ggatgaagca
     4981 aatggaataa taaaaagtga tggtactaca atattaggtt ctgatgataa agcaggtata
     5041 gcagctatat tagaaggctt aagatatata aatgaaaata acatagaaca tactgatata
     5101 caagttgtat tctctatatg tgaggaatgt ggattagttg gagctaaaaa tttagactat
     5161 ggaaaaatag attctgaata tgcatttata ttagatagtg gtggttctcc aggtgaaata
     5221 atagtaaaag ctcctgcaca agatgtaata aatgttaaga tattaggaaa gactgctcat
     5281 gcaggtcttg aacctgaagc tggaataagt gctataatgg tagctgccag agcaatagaa
     5341 aatatgaatt tacttagaat agacgaagaa acaactgcta acataggaat tataaacggt
     5401 ggaacagcta caaatatagt aacaggagaa gttaatatag ttgcagaagc cagaagtcta
     5461 aaagaaaata aattagatgt tcaaactaaa cacatggtag agacttttga aaaagctgcg
     5521 aaagattttg gagctcaaat tgagatagat gttaatagag catacacacc aatagatgtt
     5581 agtgaggatt ctgaaataat aaaacttgca aagaaggctt tttcaaactt aggtatagaa
     5641 ggtcatactg aatctacagg aggaggtagt gatacaaata tattaagtaa gaatggtatc
     5701 gaagctataa ctttaggaat tggtatgaaa aatgctcata cactaagtga acatatagct
     5761 ataaaagact tatatgattc tgctaaaatg gtagtggaga taataaagga agcataaatt
     5821 taaataatag tgtataatat atatatcgcc tagcttaagc taggtgattt ttcattttat
     5881 agaagattga taatctctat tgaaagcaaa gagtatacat ttgcatataa gagagtgttt
     5941 aatttatgtt caaatgtagg ttcttaataa tatttagatt gtttcataag ggaggaataa
     6001 atatggaaac aaggtatgaa gaagcaaata aaataacaat acaatcaata ctttggaata
     6061 tagtattaac aataattaag gttatagctg gtgttattgg taattctagc gctatgattg
     6121 cggatggatt acattcagca tcagatatta taagttctat aggagtacta ataggcaatt
     6181 atgtatcttc tagacctgga gatagagagc ataattatgg tcatgagaaa gcagagacat
     6241 tagtatcatt tgtgttatca atattgctta ttttcgtttc tataacaata ggtatagagg
     6301 caattaaatc tttatttaac ttagatgcat taagtgttcc atcaatatta ccattagtag
     6361 tatcagttat ttcaatcctg ataaaagagt atcaatatag aataacaata aaagttgcta
     6421 aaaagataaa ttcaccagcc ctaaaagctg atgcttggca tcacaggtca gatgctttat
     6481 cttcagtagc tgcttttata ggtataggtg gttctatttt agggtttaaa ccattagacc
     6541 caattgcgtc agttgtagtg gctatatttg tagctaaagt aggaattagt atacttataa
     6601 gttctgttaa tgaattgatg gatgtttcag ttgatgaaga agagataaaa gaattaaaat
     6661 ttattgtagc agatacagaa ggtgttaaaa atcttggtga tataaagact agaaaacatg
     6721 gagctatggc atatgtagat ttgactatat gtgtggatga aaatttgaca gttaagcaag
     6781 gtcatgatat tgctaccaag cttgaaaaac atataataaa acatatggaa tttgtaaaag
     6841 gtataacagt tcacgttgaa ccatgtacta attgccaagg aaataagtgt aataattaat
     6901 taaaacttaa aaaaaagtgt aaaaagttat ataattgttt tatgattata aactctaggg
     6961 gtggttaaat gagcaagaaa agaaaactaa agaaaaaagc tgcaatatta ataggagtag
     7021 ttgttttctt tataatatac tttatatcct ttagaatagg aataaatatg gaaaaggata
     7081 aaagtgaagt gccaaagaag gtagaaaaag aagaagaaga taataggtct ttaaggactc
     7141 aaataaaaga gaagaagaag gtttatatat cagataaaaa tatagaaaac ataaaaattg
     7201 aagaagatat gtgggatgaa ataaagttat ttttctcaga ttttaagaaa gtaagaaata
     7261 ctacttcttc ttacgaagca gtttatgaag gttattctga tgatggagtt aaattttcta
     7321 ctgaccttaa cttttttaga gtatatactg taaataaaga agaatattat aaagtaccag
     7381 ttagttctaa ggatgctttt aagaaacttt tagatgagag tatatatact tcgtttgacc
     7441 ttttaagtca ttataaagat tggaaagagg ttactataac tcatggaaaa gaaattaaaa
     7501 aagttcatag atggaaattt gatgatttag cacataagat gatttcaaag agggttgtag
     7561 gaaaagttca acctgagaaa agtaaggaaa gaagtgacta taattttact ataaatataa
     7621 aaggtgataa ttttgatgta aaagttgata caatgggaaa agattatgta aaagttactt
     7681 ctaagaaaaa tgtatcttac tatgaggttc atacaagttt atttgattac cttaaaaatg
     7741 agatatttga aattggaaag aaataagact ttaagaaggc tatcaaatta gatatttgat
     7801 agcctaaatt attttatata ttatttttca accaacaagt ctgcaacctt ataaattgga
     7861 ccagcaccag cagttattac taaatcatta tccttaacat tttcacgtaa gtattttact
     7921 atatcttcaa attcttttat atagatggca tctacattgt tttgatataa tttctcaacc
     7981 aagtccttag aatgtatatc tcctgggtct ttttccctag cagcatatat atctgttatg
     8041 ataactttat cagcagcata gaatgcctct gaaaattcac ctaaaagaga ttttgttcta
     8101 gtataagtat gtggctggaa gatacaccat aaagttgatt tttttaattt tttggcagca
     8161 gctaaagttg cttttaactc agttggatga tgtgcataat catctactac taaagcattc
     8221 ttatagtacc ctttagtttc aaatcttctt ccgacacctt tataaatttt tatatttttt
     8281 cttatagtct ctaagtcaat accagaaaca agagctgcca ttatggcaga agaagcattg
     8341 tatatgttat gaagaccata tacagaaagt tcaaattctc ctaaatctct tccttcatat
     8401 tctattctaa atattccatg accactattg tcaaagtgaa tatcttttat tacagcatca
     8461 ttgtcagaat ctctgccgta ttttataata gtagctttaa catcatacaa tatgtcatca
     8521 atattttcat catcgccatt tattatgaaa tagccttctg ggggtaataa ttttccaaat
     8581 ttgttaaaag aagccttgat ttcatctatt ccagagaaat aatcaagatg gtcttcttca
     8641 acatttaaaa ctatagatat ctttgggtta aaattaagga aactatctac atattcacat
     8701 gcttcagtta taaagtggtt ggaattacct atcttaacat taccacctat catacttaag
     8761 ttaccaccaa ctagtatagt tgggtctaag tctgcatatt caaagattgt agataacata
     8821 gatgttgtag aagtttttcc atgtgttcca gaaactgcta tagaattttt atattccctc
     8881 atgatttggc ctaaaaaagt tgctctgttc attaccaatt tatttttttc tttagcagcc
     8941 acaagttctt cattgtctgg atgaactgct gctgtataaa caaccatatt tacatcatct
     9001 gatatattat cttttttctg accaatataa atagttgccc cttggtccct aagtttgtct
     9061 aataggtatg attcgtttga atccgaacct gaaacttgat aacctttatt gatacatatt
     9121 tcagctaatg cactcatgct gataccgcca attcctataa agtgtatatt cataataaaa
     9181 ccacctttct ttttaattaa gtcctacaat atgaactatt tctgtggtat aataatataa
     9241 tgtt
//
LOCUS       DAEOSP010000151         9204 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.153, whole genome shotgun sequence.
ACCESSION   DAEOSP010000151 DAEOSP010000000
VERSION     DAEOSP010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9204
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.153"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            51..1934
                     /locus_tag="KRH54_002755"
     CDS             51..1934
                     /locus_tag="KRH54_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBG8580394.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IRIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMKWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            2159..2839
                     /gene="pgmB"
                     /locus_tag="KRH54_002756"
     CDS             2159..2839
                     /gene="pgmB"
                     /locus_tag="KRH54_002756"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBG8580395.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            2836..5121
                     /locus_tag="KRH54_002757"
     CDS             2836..5121
                     /locus_tag="KRH54_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBG8580396.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVARFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAISHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGR
                     PIKIIVYGKKYILEKCIKIDK"
     gene            5346..6239
                     /locus_tag="KRH54_002758"
     CDS             5346..6239
                     /locus_tag="KRH54_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBG8580397.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYSNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
     gene            6676..7167
                     /locus_tag="KRH54_002759"
     CDS             6676..7167
                     /locus_tag="KRH54_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580398.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEYEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            7195..8142
                     /locus_tag="KRH54_002760"
     CDS             7195..8142
                     /locus_tag="KRH54_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580399.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGCIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
     gene            8432..8974
                     /locus_tag="KRH54_002761"
     CDS             8432..8974
                     /locus_tag="KRH54_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG8580400.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT         3896 a          803 c         1550 g         2955 t
ORIGIN      
        1 taacctatct ttagattata taaattatta taaaggaggg aaattttaaa atgtctaata
       61 aaaataaaga aaatataact ttaagtaatc aaatcaaagg aacaattcaa aatagtttaa
      121 caaaagaagc tatattacat ataccaatga gcaattatgc ctatggttat gatagagaaa
      181 cactacatat aagacttaga actaaaaaaa atgaagcgaa aagagtaatt ttacgaatag
      241 gagaccaata tgtgtgggat aaaggaggtg caggaggagg aaatttaaat gcatctggag
      301 ttggttggtc tggtggaaca aatatagtaa tgagtaaaga agtagaaact gaattatttg
      361 actattggat agctaagtgt aagcctttaa ataaacgctc acgatatggt tttataatag
      421 aaggtcaaga ggaaaaactt ttatttacag aaaaaagaat aatagagttg ggaaatgaaa
      481 atgatgaaaa ggaattatgt gaaataggta atttttttgg atatccatat ttaaattata
      541 tagatatacc taaagtgcca aattgggtga aagatactgt ttggtatcag atatttccag
      601 atagatttgc aaatggtgac ccgtctataa acccagaagg agtagataaa tggggagcta
      661 ttccaaccag agataatttt acaggtggag atttacaagg tgtgatagaa cacttagatt
      721 atttgagtga tttgggaata aacggaattt acttttgccc tattacaatt ggaaaaacca
      781 atcatagata tgatacggta gattatatgg aggtagaccc tacattagga gataaagaga
      841 ctttaaaaaa actaatagaa gaagcgcata agagaaatat aagaataatg ttggatgctg
      901 tttttaatca tataggatat tattctaaac agtggcaaga tgtagttcaa aataaggaaa
      961 attctcgata taaaaactgg ttttatataa aagatatgag taaaattgat actccaatag
     1021 aacaaataga tgaaaaaaat ataccttatg aaacctttgg gtgtgaaaaa tatatgccta
     1081 aacttaatac agaaaattca gaagtaataa aatatttatt ggatgtaggc aagtactgga
     1141 tacaggaatt tgatatagat gcgtggagat tagatgtttc aaatgaagta gaccatgttt
     1201 tttggagaaa gtttagagaa gaagtcaaaa aagtaaaacc agacatttat atattgggag
     1261 aaatttggca tggtagttta ccatggctaa tgggtgacca atttgattca gttatgaatt
     1321 acttgatgtc agaagccatg aaaaaattct tttgtacaga tgagataaac gccgaagaat
     1381 ttaagtatat gataaatgat gtaatggtaa gttatccaat acaagttagt gaagttattt
     1441 ttaatttact tggaagtcac gatactacaa gaatattgac ttatgctaat ggaaatattg
     1501 atagatttaa acttgcatat atgtttatgt ttgtacaaac aggatgtcct tgtatatact
     1561 atggtgatga aatcggaatg gaaggagaat taagttcaat atctgaaggt caaagaaaat
     1621 gtatgaaatg gaatgaagaa aaatggaata aggatatatt tgattttatg aagaagatta
     1681 ttagacttcg taaagaaaat aaagaactta ggtctatatc aaatgaatgg gtgttagcag
     1741 ataaggaaaa tggaacgatt atattgaaaa aagaatctat ttctataata ataaataatt
     1801 catctaaaaa tataattttg acgttaccag attatttagc aaataaaaaa gtaaaagatt
     1861 tatatgaaga agaaatagtt gatttaaaag aagaactaat gttagagaaa tacaagttca
     1921 ttattttaaa ataatacaaa aaagtattta ttttagattt catgttaaat tattatacag
     1981 tttaaataag tctttataaa cgataaaaaa atatggtcta caagtgagtg aattgaaagt
     2041 agattttaaa ttaaagactg agttagagta gataatttta taaataatat cttaaataca
     2101 agatagaaaa taaattgatt attagtctaa aaatttcttg tagaaagagg aaataagaat
     2161 gattgaagca tttatatttg atttagatgg tgtaattaca gatactgcat attatcacta
     2221 tatggcttgg agaaaactag cccataaagt tgggatagat atagacacaa aatttaatga
     2281 atctttaaaa ggcataagta gaatggagtc attggacagg atattagaat ttggaaataa
     2341 gaaatattca ttttcagaag aagaaaaagt gagaatggct gaagaaaaaa ataattacta
     2401 tgtatcattg atagatgaga taacttcaaa tgatattttg ccaggtatag aaagcttgtt
     2461 aattgatgta aaatctaata atataaagat aggtctatct tctgcaagta agaatgcaat
     2521 aaatgtatta aatcatttgg gtataagtga taaatttgat tttatagctg atgcaggaaa
     2581 atgtaaaaac aataaaccac atccagagat atttcttatg tcagcgaaag ggcttaatgt
     2641 aaatccacaa aattgcatag ggatagagga tgcaagtgca ggtatagacg ctataaactc
     2701 agctaatatg ttttctgttg gtgttggaaa ttatgaaaac ttaaaaaaag caaatttagt
     2761 tgtagattca acaaatcaat taaagtttga atatatacaa gaaaagtata atgagtatat
     2821 agttaggaga attatatgaa atgtttattt agtaatgtag atgagtggaa gattatacaa
     2881 gataagattg aaatcaaaga aaatagatta gcagagtcca taatgagtat aggcaatgga
     2941 tatatgggta tgagaggaaa ctatgaggaa aattatagtg gagatacaca caaggggtct
     3001 tatatagctg gagtatggtt tccagataaa actcgtgttg gttggtggaa aaatgggtat
     3061 ccagaatact ttggtaaaat tcctaattca gtcaactata taggtgtaag aattttcata
     3121 gatgatgtag aattagattt agcaaaatgt aaagtagaag atttttatag agaattagat
     3181 atgaaaaatg gtattttaaa tcgtaggttt attgtaaata taaatgataa aaaatttgaa
     3241 gttagggttg ctaggttttt aagtatatct gttaaggaac ttgcagtaat taaatacagt
     3301 ataaaatcat taaattctaa tactaaaata aaatttgtac catatttaga ttcaaatata
     3361 aaaaatgaag attcaaacta tgaggaaaag ttttggaatg gaatttcttc aagttcttta
     3421 gaatataaag ggagtattct ttctcgaaca aaggagaatg catttaatac accagtattc
     3481 actgtgggtt caatgatgta tattaagaca aatggtgatg tagaagctat atctcatgag
     3541 agtgattata attactgtga aaattcgata acaatggatg ttttaaaaaa tgaggttgca
     3601 agtatagaaa aatatgtatc tataacttca actagagatt ataaggaaag tgagctatta
     3661 agaaaatgtg aagacatttt aaatagagaa ctatctaaaa gttatgatga tatattaaat
     3721 gaacatacaa agttatggaa taaaagatgg gaaagtgcag atgtaaaaat aaatggagat
     3781 aaaagtagcc aacaaggtat aagatataat ttattccaac tattttcaac atattatgga
     3841 gaagattcgc gattaaatat aggtccaaaa ggatttactg gtgaaaagta tggaggagct
     3901 acttattggg atacagaagc attttgtgtt ccagtatatc taggtgtagc agataaggag
     3961 gtaactagaa atcttttaga atataggtac aatcagttag aacaggcaaa ggataatgca
     4021 aaaaaactag ggcttaaagg tgctttatat ccaatggtaa catttgatgg agtagaatgt
     4081 cataatgaat gggagataac atttgaggaa atccaccgaa atggaagtat agtatatgca
     4141 atatataact atacaaatta tacaggtgac tataaatata taaaagaaaa aggaatagat
     4201 gtaataatag aaatagctag attttgggca agtagagttc atttgtctac aaaaaaagat
     4261 ttgtatatga tacatggagt aacaggtcca aacgagtatg aaaacaatat caataataat
     4321 tggtatacta attatatagc taaatggtgt ttagattatg ctgtagaaaa tatcttacga
     4381 ttagagaaag agtgtaatga atgtattgac agaaataatg tgactaggga agaagttgag
     4441 ttttggaaaa taatatcaaa taaaatgtat ctgccgtatg atgaggaact acaaataata
     4501 attcaaaatg ataatttttt agataaagaa tttgtagaag taaaggattt accaaaagaa
     4561 aattttcctc ttaatcaaaa ttggtcttgg gataagattc ttaggtcgtg ttttataaaa
     4621 caagcagatg tattacaggg aatttactat tttggaaata gatttactaa agaagaaaaa
     4681 aagagaaatt ttgacttcta tgaaaaatat actgtacatg aatcttcttt atcttctagt
     4741 atttattcta ttattgcctc agaaatagat aacctagaaa aggcttatga attatattca
     4801 agaacagcta ggcttgattt agataattac aataatgata caaatgatgg actgcacata
     4861 acttcaatgt ctggttcatg gctttccatt gtacatggat ttgcaggcat gagaacatgg
     4921 aatgaaactc tatcttttga ccctaagttg ccaaaagagt ggaaaaatta ttctttcaat
     4981 ataaattata gagataatat tattaatgta gatgtatgta gtgatttaat taaaatagaa
     5041 aatttaaaag gtagaccaat aaaaattatt gtatatggga aaaaatatat attagaaaaa
     5101 tgtataaaaa ttgataaata gatttaataa tatttttatt atataaatag ttaaaataag
     5161 ctatctaatt atttatttat tttaactatt ttgtgtgata gaaaactata tattataaca
     5221 atagtagtgc ttttaattaa atatatttca taaatcttca taaaatcttc aaaatttcgt
     5281 aaagaatatg tcacaaaagt aaggtatatt atattcagca gatagtataa gggaggacaa
     5341 tatttatgaa gaataaaaaa ttcattgtta taatactgat aatatcaatt ttaataggtg
     5401 tattagtgaa ggaatattca tctagagaga taaaaaagga tgatataaat gtcagtaagt
     5461 atataaaata tgctgattta gcaagtaaaa ataatgcaca agttaactgg aagtatgtag
     5521 cttccattgt tgctgtgtta aataaaaata acttaaaaaa cgttaaggat tcacagatac
     5581 aagaagtatc agatttattt gttaagaatt tttctaaaaa taataaaata aataagttaa
     5641 gtgatatttt ggatgagtta gaatttagta atagacaaaa aagattggtt gataattata
     5701 tagacaatct taaggattat ggtataaaac cagaaagatt gaagtctgat acaaaatata
     5761 tgaagtttat tgatgaaatt aaaacagaag caatacaaaa ttataaggat tataaaatac
     5821 ttccatctat aactattgca caagctataa tagaatctag ctggggcaag tcaactttgg
     5881 caaaacaata tagcaatcta tttggtataa aggctgacgc ttattggaaa ggtaagtcag
     5941 tgactttaga aacgaaagaa catttagata caattataga tgataaattt agaatatatg
     6001 atgataaaaa tgaatctata aaagaccatg caaaattttt agccacaaat aaaaggtata
     6061 aaaacaatgg tgtatttgat gcaaaaacgt atatatatca agctaaagca ttagaaaagg
     6121 caggttatag tactgcgaag gatgaaaatg gcaatagtat atatgcagct cgtctaatag
     6181 aattaattca acagtataat cttcaattaa tagacagtga aattcaaagt gaaatgtaac
     6241 tttgattaaa tttttattga taaggctatg atttttaaaa tcatagcctt attttgacat
     6301 gcttttactt gtaattatta aatcgatatc attatctttc aaaatttact ttatactgta
     6361 aatcaaatta tttaagttaa aacttggaat tatttataaa aggattattt actttatcta
     6421 aattctgcac ttaaagaaca tcttttccct taagactgac cgttagtcta taaaaattat
     6481 aattacttat aaaaaagtga atatatatat aaagttttca gaaagacttt tacttgtaaa
     6541 ttgaaatcaa tgaagtaaaa aaggattctt tcttaaaagt aaataacaac tagatagtga
     6601 ataataaaaa atggtaattt gaaataattt ttatgaatag tattaatata tagatacagg
     6661 gagattgatg cgattatgta tgaaaataat aaggtaatat acaaccttga aagatgtgaa
     6721 aaactagaaa agactcttta tttatctcaa tttaatacat ataaaaattt taaattagct
     6781 tctaaaaaaa ctgtaataaa ttcattagag acatatgttt acaatatgaa tattaacaat
     6841 ataagtcttg ttgatactat agaatgtata tcattagaat atgaacaact tacaggaaag
     6901 aagttgattt tagttggtga tatagatatt gatgcaatat tagattatgc aggaaataaa
     6961 agaaatagaa attctagtaa aaaaaataca tttaaattaa aaataccctt tagtacattt
     7021 atacaaatgc ctagaaagat agaaaataaa gataaaatta atttaaagta tcttatacaa
     7081 gatattactt catctatttt agatgataat ttatttatta gtgttactgc aattataagt
     7141 tatgaaaata gttccaagat agagtaatct ttaaattata gaaaggtgga tgcaatgaga
     7201 ataacgaaaa ctacttttaa tgatgtattt tcaaatgatg caaatggaag tattataaaa
     7261 gtagaaaaat atgttgataa aaaatgtgca tctgtatttg agattttgac ctacactatt
     7321 attgttacaa atacaagtag gtacaaaact ggaaatatat ttttcaaaga ttatatttct
     7381 aaatatatag agtttataaa taatactgta aaagttaatg gcattataaa acgtggatta
     7441 gacccacaac aaggttttta catagggaga atagatgcaa gttgtaaaaa aataatatct
     7501 tttaagagtg tagttttacc aaattctgca cataggatta taaagaatag tgctaatata
     7561 tactattatt ataagtgtaa tctagataaa ttcccaacaa gaatatcaat agaaagtaat
     7621 agagtttata ctaatgtaaa taaaattgta ttcaaacaat tgaatataag taatacttta
     7681 aaagctccta aaaatctaag agatatatta aaagtaaatg taaatagtaa aatatttgat
     7741 gttaaaccta tgaaaaatct tataaacaaa ggtagtaatt tgaaattatg taacttgata
     7801 gtttttgggt gtatagaagt tgaaatagac tatagttgta aaaataggag taattttaaa
     7861 acagtaaata agtctaatga cattaaaaaa gataagtatt ataaaaatga tatacaaaat
     7921 aacaatgaaa atataaacga ttgtaaaaag caaataagtt ttggtgataa taaggtaaaa
     7981 aaaataacaa aaacttttgg attttcttgt ttcatttgtt ctccagttgg aattatttat
     8041 gaagatatga aaaatataaa tataaaaatt gaacatacat caataaatga attaaatcca
     8101 gaagaattat ttgtaaatac atcactttta ttgtattatt aagtatttta aagctaaaac
     8161 ttaaacttat taaaatagca tttggttaaa ggaaaataga tattgatata tttatcatat
     8221 atataaatga ttaattaaac aattataaat aataagtata gtataatatt taaaaaatat
     8281 agatttatga taaaataaga caaatatgtt aaaaaaatga taatcacgga gaacttttac
     8341 cttcttcaat taatatatta ttattagtaa taactttaaa attatattac tattatataa
     8401 aaattggctt aaaagaaggt gaaaaaagta aatgaaaata tgcataacag taggtcatag
     8461 tatactaaaa ggtggtaaaa gtacaggtgt aaatggtata gttgatgaat acaattataa
     8521 taaaaaatta gctcctatgt tagctgagat gcttataagt caaggtaata cagtagatgt
     8581 aataatttgt ccagaaaggc actttatgtc agaaagagaa gaatttttct atagagttcc
     8641 aaaagtaaac agtggtaaat acgatttatt agtagagcta catttaaata aagctgatgg
     8701 aacacaatgt ggtacagaag tgctttacta tggcactgaa ggattagaat atgctaaaag
     8761 agtatctaat agacttggag aattatttga aaatagagga gctaaaaaaa gagaaaattt
     8821 atatatattg agaaatacaa atccagttgc aatacagatt gaaagttttt tctgtgataa
     8881 tgtgaatgat tgtaacaaag caaatgaatc aggttatgat tatatagcac gattgattac
     8941 agaaggtata ttgaataaag atataaatat gtgagattat ggtgatttag gtattgatta
     9001 atacctttta atattagata taaataaaaa tacaatgtat tagtataatt taaactttca
     9061 aaagatagat tagtttgatt attgacaaga gatacctcct tgaaaatatt gatttatcag
     9121 tatatttaag gaggtattta aatataaaaa tttaattaat ttgctactaa actataggtt
     9181 tatagagtat atacttttat cttc
//
LOCUS       DAEOSP010000152         9176 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.154, whole genome shotgun sequence.
ACCESSION   DAEOSP010000152 DAEOSP010000000
VERSION     DAEOSP010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9176
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.154"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            302..643
                     /locus_tag="KRH54_002762"
     CDS             302..643
                     /locus_tag="KRH54_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBG8580401.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            794..1207
                     /locus_tag="KRH54_002763"
     CDS             794..1207
                     /locus_tag="KRH54_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBG8580402.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1409..2050
                     /locus_tag="KRH54_002764"
     CDS             1409..2050
                     /locus_tag="KRH54_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBG8580403.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            2074..3327
                     /locus_tag="KRH54_002765"
     CDS             2074..3327
                     /locus_tag="KRH54_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG8580404.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3373..4023
                     /locus_tag="KRH54_002766"
     CDS             3373..4023
                     /locus_tag="KRH54_002766"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBG8580405.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            4074..4670
                     /locus_tag="KRH54_002767"
     CDS             4074..4670
                     /locus_tag="KRH54_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG8580406.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4683..5522
                     /locus_tag="KRH54_002768"
     CDS             4683..5522
                     /locus_tag="KRH54_002768"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBG8580407.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5767..6921
                     /locus_tag="KRH54_002769"
     CDS             5767..6921
                     /locus_tag="KRH54_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBG8580408.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            7227..8924
                     /gene="ade"
                     /locus_tag="KRH54_002770"
     CDS             7227..8924
                     /gene="ade"
                     /locus_tag="KRH54_002770"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBG8580409.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGVSAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMEVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
BASE COUNT         3767 a          872 c         1593 g         2944 t
ORIGIN      
        1 taagataaat tttaatacga ttcatttaaa ttaaaagtta ataattactt ttttatgtat
       61 aattttacat gaatttctat aaaagttatt ttattaaaga tagatatact tatatatacg
      121 gatataatta aaaaaagtct ttttaacttt aaaaattaat gttaaaaaat aaacttatac
      181 attaatatta ataataatta tttaaatatc ttaagtaaat cagtcttaca tatatttccg
      241 atagattata ttgataaaaa acactaaaat taaattaata aaagtacttg gagggatgga
      301 aatgaaagag gaaaatttta ttaatgttga atacgaggat aaacgttttt caattaatag
      361 taaagaagtt ttgtacatag aaaagcaaga gtatgaagta aagatttata ctgaaagtgg
      421 taattatgaa gttggagaaa atgctccttg tataagaaaa ctattgaaag aagaaaattt
      481 tttcaaatgc agtaatagtt atattgtaaa tctagacaag tgtgaatttt atctcagcaa
      541 taattgtatt tttgtgaata ataattttat aagaatttta ggttcagaaa gaaaaaagaa
      601 attaaaaaag aaattagaaa aattatatga ttatgaagat taatattggg aggctgtatt
      661 ttatacagcc tctttattta ttgattgtat tattaataaa ttttaaatta tgataaaata
      721 taaaacaagt aacttatttc agaataaaat actttttaaa agaaagggac gagatatggg
      781 agattataat aaaatgcttg aattaaagaa taacttaggt atcagtgaaa gaaagttaaa
      841 atatccttgt atctataaac atttcaaagg gaaatattat gctacaatgg gtcttagcaa
      901 ggctattgat gatatagaaa acatatgtga aatatatggt aaagaaaatt taatccaaaa
      961 cagaaataaa tataaattag ttattagaca tactgaaaga gaagaagata tctatgttta
     1021 tagggattta gatggaaact tttatcataa aaaagaagaa gatactaatg atttagtttt
     1081 atacaagacc ttatatgatg atacaggaat atttgcaaga cctttggata tgtttttaga
     1141 aaaagtagat acagataagt atgtcaattc aattcaagaa tacagatttg aagaagtata
     1201 taaataatat aagagaaata tgtttataaa tttagaaaaa ttctttaaca gtacaataat
     1261 ttgcagaaat acaaatgaga tttattgtaa ataatatgtt agattagatt ataaaataga
     1321 aacaaagtaa agtattatat gatataatta agttttgaga agtgataatt tatatttaat
     1381 aaaaaatagg catatattag gaggacatat gggaaatact ttttatatag taagacatgg
     1441 acaaacggat tggaatatat tgggaaaaac acaaggtcat ggtaattctg accttacgcc
     1501 tcaaggtata gaacaggcaa aagaactttc tgaagatatt ggcaagtatt caattgatta
     1561 tatttttagt agcgaccttg ggagagcaat gcaaacagct caaattttag gagataagct
     1621 caatattgaa gttcaaaaaa ctgaggcact tagagaaatg ggattcggag tttgggaagg
     1681 ccttttaata aaagaaattc aaaaagatta ttcagatata tatgcaacat ggagaaatga
     1741 accacactta gtaaatatac cagaaggcga aactcttaag attataaagg aaagagtaga
     1801 tgcttttata aaggaactaa atgaaaaata tgataataaa aatataattc ttgttacaca
     1861 ttcaattaca ttgagggtta tgttgttgtc atttttagaa tcaggtatgg aaaatatata
     1921 tagaattaag caagataata ctgctttaaa tatagtagaa tttaaagatt atgggcctgt
     1981 tatagtaaaa atgaatgata caagtcatat aaaaaatcat gtaaagataa ataattctgc
     2041 actggagtag gaatcaatta taaggagaaa tttatgagta aagttatagt agttggtgga
     2101 ggagcttctg gaatgatggc agctttgtca gcttcaaaaa acaataatga agtaatatta
     2161 gttgagagaa atggagaatt aggaagaaaa cttagggcaa ctggtggcgg tagatgcaat
     2221 tttaccaata atagagagat agaagatttt tttgataaag ttgtcagtaa taaaaaattt
     2281 ctctatagta gtttttatac atttacaaat aaagacctta tttcgtattt tgaaagtaga
     2341 aatttggaat ataagattga agaagaaaat gaccacaaag tatacactaa aaatgataaa
     2401 tctatagaag taatagaagt attaaataaa gatttactca atcacaatgt aaagataatg
     2461 tataataaga aagttataga tattataact gaagaaattg ctctaaaaga tgattctaat
     2521 aaggacaagt caaagtatct aataaaaggg attattttag ataatggaga taaaatacta
     2581 ggagacaagg ttattatatc aactggagga gtgagttatt caaagacggg ttcagatggt
     2641 tcaatgtata agattcttaa aaagcatgga catacattaa acaaactata tcctgcttta
     2701 gttccactta caatagagga aaaatggatt aaagatttac aaggaatatc catgaagaat
     2761 gtggaaatat cttgtaagat taagaaaagg aaaatatcaa aatctggaga catgctattt
     2821 gcccattttg ggataacagg accttgtgtt cttataatgt cttcttatat taacaagatt
     2881 attgaaaagg aaaaggtaga attaaatata gatttcttac caaatttaag tactgatgaa
     2941 atctctagta taatcagagc atttccaaat aaaaatgttc taaataatct taagcaaata
     3001 ctacctcaaa atttcttaaa agagattttt tctctattat ctttagttga taaaaaggca
     3061 agtgatttgt ctaaggcaga tgaaattaga attattgagt atataaaaaa tatgaagcta
     3121 acttgtaatg ggactacagg tataaacact ggtatggtta cttctggtgg tatttcagtt
     3181 aaagaaatta attcttctac tatggaatct aaattagtta agaacttatt ttttacaggt
     3241 gaagtaattg atatagatgc agaaactggt ggatataatt tacaaatagc attttcaact
     3301 ggatatcttg cgggtataag tgtataaata taatttttta ataattaaat atgaataaat
     3361 agaggtgtat atatgggtaa tttagttata gctgtagatg gtccagcagg agctggaaaa
     3421 agtacaatag ccaagatagt agcaaaaaag ctaaatataa attatataga tacaggtgct
     3481 atgtacagag cagttactta taagtgttta aaaagtggta ttgatgtcaa taatgaaaaa
     3541 gaagttattc aaatagctga aaattcggat atagatttta aagataacaa tatatatttg
     3601 gacaaagaag ttataaatga agaaataaga acaatagaag taagcaataa tgtttctaat
     3661 gtagcaaaaa taaaagaagt aagacagttg atggttgaag ttcaacgaaa aataggaatg
     3721 aaaaattcag taatactaga tggtagagat ataggttcat atgttttccc agatgcagat
     3781 tacaaattct ttttagtagc tactccagaa gaaagaggaa atagacgcta taaagaactt
     3841 tgtaataaag gatataatac tactttagaa gaagtaattg aggatataat aagacgagat
     3901 gaaattgatt caaatagaga atttgcacca ttggtaaaag ctaatgatgc actagaaata
     3961 gatactacag gaaaaactat agaagaagtt gtagaagaag ttgtatctaa aataaatctt
     4021 tagtttttta tgcatcataa tgaaaataaa tgtagtgttg aagggagata aacgtgaatt
     4081 tttatagatt tgtaataaat atttttaagg gtttttctaa aatatttttt aaatatgaag
     4141 ttataggtgc agagaatatt ccagatagag gaaatatagt aatagcatca aatcataaat
     4201 cgaatcttga ccctatattc ttggctgctg ccatagaaaa tagagaaata gctgcaattg
     4261 caaagaaaga attgtttaaa gtaaaaccat tgggatttat attaaaaaaa ttgcatgtta
     4321 tgcctataaa tagagaaaaa ccagatgtat ctacaataaa aactatttta aggtcagtta
     4381 gagacggtta tgtattagga atatttccag agggtactag aataaaagga gattcttttg
     4441 gtaaggctaa ggcaggtctt tctgtattta caataaaaag taaatctaaa gttgttccag
     4501 tatcaataat atctaaatat aaacttttta gtaaagtaat tgtatatatt ggagaaccaa
     4561 tatcatttga agaacatttt aaggaaaaat tgagtaatga tgaccatgaa aggatatcac
     4621 aagagatttt agaagtgata aaacaaaatt actttaaata ttcaaaataa gggggtaaag
     4681 ttatgaacat aaaaatagca aaaaatgctg gcttttgttt tggagtaaaa agagcaatga
     4741 aaatggcatg ggatgaagtt gaaaaaaatg attctggcat atatgcttta ggtcctctta
     4801 ttcataacaa gcaagcagta gcaaaatatg aagaaaaagg tcttaagact gtgaatgaaa
     4861 ttgatacaat accaaatcat gaaaatatga taataaggtc tcatggagtt cctgaaaata
     4921 tatataagga agccaaagat aaaaaattaa aaattgtgga tactacatgt ccatttgtaa
     4981 agaaaataca tacagtagta agtgaatatc ataataaagg ctatgaaata atagttattg
     5041 gagatatgaa gcatccagaa gttataggta taaatggatg gtgtgaaaat tcagctataa
     5101 taataaagac cttagaacaa atggaaaata tggaatttga taactccaaa agatattgtc
     5161 ttgttgcaca gactacaata aatccagagt tgtatataag tatagttaat aaactatcag
     5221 ataaattaga agaaatagta tttaatgata caatttgttc agctacaaaa acaagacaag
     5281 aatcagctaa agagttagct aaagaagttg attgtatgat agttatagga ggtaaacaca
     5341 gttcaaatac tcaaaagtta gtaaaggttt gtgaagactt agtaccaact tttgcaatag
     5401 agactaaaga tgaattggat gtaaatacgc ttaaaaaata taaaaatttg ggtattacag
     5461 caggggcatc tacacctaat tggataatag aagaagttgt aacattcctt gaaaatttat
     5521 aatttgatag gtaataaaaa taggtggaca ttgttataat ttatcataat atccaccttt
     5581 gatttcatat tacatgtggt aaatagataa cctaatatca aaaagtgtgt atcttaagat
     5641 gaaaaactaa gagtataaga tgcgcacttt ttaatattag aaattcaaaa gaaatattga
     5701 tattgattat caatagaaaa tagaatataa tataattgtt gcaaaattag tctaggaggt
     5761 aaattaatgg aaatgttgat atccgcttct gaagaggcgt ttttacatgt aggttctatg
     5821 attggatttt ttattttgtt atttggatat attaattata aaactagtgg aaattttact
     5881 aatatcatat caaaaaacag aaaatttcaa cccttaatag gtgctttaat tggagctata
     5941 ccaggttgtg gcggaagttt ggcaataatg ccattgtata taaatggaaa attaagcttt
     6001 ggagcaataa ttgcaagttt aatagctagt atgggagatg cagcatttgt acttatttca
     6061 tcaaatatta aaatgtattt ttttgtcact atagttagta caattacagg aataataact
     6121 ggacaattag tggattactt taaactagaa gaaaaattag ggttaaaaaa ccgtagaaaa
     6181 tcagataaat atgatataag taaaaataac cctaaagaaa ataaagaaca acataatcat
     6241 gatgaaataa ttttagacac tctagctaaa tctcatggaa atacaaatag acttgcattt
     6301 ataataacac atggaaaggg atataaaatt tatataggaa ttatagtaat cggttttata
     6361 tttatgtcac ttgctcactc tggacttaat cttcctataa ttgagaagtt acattcattg
     6421 gaagaagtaa tagcagtgat tggaatatta ttttcaataa tttatatgtg gtgttttaaa
     6481 aaggtattta aaaattctaa tcaaaaagag gaagaaaata aaaagatttc tttgcgagaa
     6541 atgcttatcc attctgttgg agaaatatca tttgtaatta catggatttt tattgcatat
     6601 cttatttacg atttgataat attaatttta ggtggagatg aatacttagt taaattagta
     6661 ttatcaacag gtgtaataag tgtatttata ggtgcaggtc ttggacttat tccaggttgt
     6721 gggatacaaa ttgtgcttat gtcattttat ttgaagggaa ctataccact tggtgcagtg
     6781 atagccaact ctatttctca agatggagat gcacttttcc cacttttagc aatggataaa
     6841 aaatcttctt tatgggctat gattattact actataccag caatacttgt aggtatgata
     6901 gtttatgcat ttttaggtta aatataatta tgaataaaga agtattaaaa aaataataaa
     6961 gcacataaat ttatttataa gaatagtgaa gattaaatat aaaaataaaa aattcaatat
     7021 gaattaaatt attgcttaaa tattaatgag aacatgggag ataatattat tcctcatgtt
     7081 ctctctattt aattaaagga attatggaac aaatttcaag tgatactttc tatcaaaact
     7141 catatataat atatattaat aaattaaact ataatacgta atacagtcag tatattgata
     7201 taaaaaactt aggaggagtt gtaaaaatgg gtaatttaaa aagacagtgt gatgtttcaa
     7261 gtggaagaga aaaagctgac attgtactaa aaaatgggac aatcataaat gtatttacag
     7321 aagagttaat aacaggagat gtagcaatag taggagacac tattgttgga attggcgatt
     7381 acaagggcaa tgtagaaatt gattgtagta acaaatatat atcacctggc tttattgatg
     7441 cacatatgca tattgaatct acaatggtaa tgccaattga attatcgaaa aaattattaa
     7501 aatcaggtac gacaactatc atagccgacc cacatgagtt ggtcaatgta aaaggagtta
     7561 gtgcaataga ttttttatta gaatcgacaa aagatatacc acttaatgta tatattatgg
     7621 tgccttcatc cgtacctgca acaagttttg aaactaatgg agttggtaaa tttagtgcaa
     7681 aagacatgga gagttatgta aataatccta gaattttagg tttaggagag gtaatgtgtt
     7741 ttaatgatgt tataaattct gaaaatgaaa tcttggataa actagagctt tttaaaaata
     7801 aagttgtaga tggacatgca cctaatataa atggtaaatc acttcagaca tatgtatgtg
     7861 caggaataga aaatgaccat gaatgtataa cttttgatga agtatatgaa aaattgagag
     7921 caggattaaa aattttaata agagaaggaa gcgcagcaaa aaatttaaag tctatagtaa
     7981 gtggtatgct aaagcataac ttgccaattg aagaatttat gttttgcaca gatgataagc
     8041 acttagatga tatagaaaaa caaggtcaca taagatggaa tataaaatgt gcgattgatt
     8101 taggtatgga acctgttagg gctataaaag ttgcaactta taattctgca aaagcatatg
     8161 ggttaaggaa aattggagca ataggtgcgg gttataaggc agatattgtt gtattgaatg
     8221 atttagacaa aatggaagtg gatagtgtat ataaagatgg taatttggta aacgaagaga
     8281 tgttttctaa ttacaattat gagattaaag ataaagaatt gcttaataca gtcaagttta
     8341 aatatattaa taaagaaaaa atacagttaa aagtaagtga aaaaaattat gtaatggaga
     8401 ttgtacctta tcaaattttg acaaataagg tatatgaatc tttaccatgt gcagatggat
     8461 attttgttcc aaataaagag tattcaaaac tttgtgttgt tgagagacat agaatgactg
     8521 gaaatgtagc aattgctcca ctaaaaggat ttggcataaa aaatggagca attgctacta
     8581 ctgtggccca tgattctcat aatattatag ttgcagggga taatgatgat gatattctag
     8641 tagctataaa ttacttaaag gaaatacaag gtggttatgt aattgtatca aatggaaaag
     8701 ttcttgcaca cctaagtctt caagtagctg gattaattag tacttttaca gctgaagaag
     8761 tacaagaaat tactgataat atgttggaga tagcaagaaa aatggaagtt ccagagtatg
     8821 tagacccttt tattacactt tcttttatgg cacttccagt tgttccacaa ataagactta
     8881 cagatttagg tctatttgat gttgaggaat ttaaatttat atagatagta taaaagtaat
     8941 aataaatgag gctttctcaa aatagagata agtctctttt tattgttgaa atgatgaaaa
     9001 tatatttgat ttcatacaaa aaagagcatc tcatgaacta aaaattttat ttttagatat
     9061 ccttatttta attaatctat catttaacat tgttttaagc atagatatct taataaaact
     9121 attttcagtc tattttaaca tataaataca tgaaaattat atatttatga aacttt
//
LOCUS       DAEOSP010000153         9153 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.155, whole genome shotgun sequence.
ACCESSION   DAEOSP010000153 DAEOSP010000000
VERSION     DAEOSP010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9153
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.155"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(103..1047)
                     /gene="manA"
                     /locus_tag="KRH54_002771"
     CDS             complement(103..1047)
                     /gene="manA"
                     /locus_tag="KRH54_002771"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBG8580410.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            1220..2581
                     /locus_tag="KRH54_002772"
     CDS             1220..2581
                     /locus_tag="KRH54_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG8580411.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            2669..4558
                     /locus_tag="KRH54_002773"
     CDS             2669..4558
                     /locus_tag="KRH54_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG8580412.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISTVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKANTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            4573..5028
                     /locus_tag="KRH54_002774"
     CDS             4573..5028
                     /locus_tag="KRH54_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8580413.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            5087..5404
                     /locus_tag="KRH54_002775"
     CDS             5087..5404
                     /locus_tag="KRH54_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG8580414.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            5429..6691
                     /locus_tag="KRH54_002776"
     CDS             5429..6691
                     /locus_tag="KRH54_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG8580415.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            6796..7860
                     /locus_tag="KRH54_002777"
     CDS             6796..7860
                     /locus_tag="KRH54_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG8580416.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            7914..9092
                     /locus_tag="KRH54_002778"
     CDS             7914..9092
                     /locus_tag="KRH54_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBG8580417.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
BASE COUNT         3635 a          934 c         1608 g         2976 t
ORIGIN      
        1 aaataaaata aaaatactat ttgtttaaat atttaagttg taagaaatag gctatcataa
       61 ggtaaattat tatgatagcc tatttttagc taggttttat atctatatgt gagaacatat
      121 aatctctaaa ttaccttcaa aactaaaatc tccaaagtcg tttggtagta taaaatgcat
      181 accctttttt aaattgtatt ctacagaatt gtgtataagt tttccatttc catccaacac
      241 actgtataaa gaaaaaggtg tattgtgtgt aaaattacat tttccaaaaa catctaattt
      301 ataaacacta aaaaattcgt ttgacacaaa tgttgtgcat ttaaagtctg atgttgatac
      361 gattttatag tcagtatcga aatttatatg aggaacattc gtaacatcta ttgatttttg
      421 aacatgaagt tcacgcttat tacctgaatt gtcagttcta tcataatcat agactctata
      481 agtagtatct gagttttgtt gagtttctaa aatcaatgtt cctttgcata tagcatgtat
      541 agtgccactt ggaacataga aaaagtctcc cttttttata ttaacctttc taagtagttt
      601 gtcccattca ttattattta ccatatttac aaattcttta tgagattttg cattatgacc
      661 tattataatt tctgcatcat cagaacaatc tattacatac caacactctg tcttacctaa
      721 ctctccattt tcattttttt tagcatattc atcatttgga tgaacttgaa ctgataaatt
      781 atcatttgca tccaatattt ttgtaagtaa aggaaactta tcaccaggtg tgtttccaaa
      841 taattctcta tttttattcc acaattcaga tagtttttta cctttatatt ttccattttc
      901 tattaagcaa tctccatttt tatgagagct tatagcccaa cattcaccag ttgttggcga
      961 atctatttca taattaaatt tatctttaag tgcagtacca ccccatattc tatccataaa
     1021 aataggtttt aaaaataatg gttccataat cctgttcctc ctatgatatt aaatttatcc
     1081 tacaaataat tatatataaa tagattataa aatcaatagt aaaaaagttt tgtcccttga
     1141 aatgacataa agtataagga gttattaaat aagataaact atgatatata atataaagtt
     1201 ctaaattgga gtgataatta tggagttgtt taaggcagaa agcaaaaaat ataaaaaaga
     1261 atttttaaaa ttattggagc aatgggttag tataccttca ttttatgata gaaaaactgt
     1321 atctaaagac atgccttttg gaaaaggagt atatgatgca ttaaactggt ttgaaaatct
     1381 aggtagagaa aacaatttca aagtaaaaaa tatagataat catgctgttc aaattgaata
     1441 tggaaatggt aaagagtatg tagatatatt tggacattgt gatgttgtaa atccaggtga
     1501 aggatgggat agtgaaccat ttaaactaaa tattataggt gataaattag ttgctagagg
     1561 tgtatctgat aataaagggc ctatgatagt gaatttttta gcactaaaaa tgataaagga
     1621 tttagatatt aatttaaaaa gaaaggttag attaatagct gggggaaatg aagagagtgg
     1681 atttaaatgt ataaagcatt attatagcaa agagccatat ggtgtatgtg gatttactcc
     1741 agatgctaaa tttcctgtat taaatggaga aaaaggtgga gcaataataa agttaatttc
     1801 aaatatagac gataaatctt tatatataag tggtggaata gagtttaata caataccaga
     1861 taaagtttat ataaagaatg tagaaaaatt gggtaaagat aatatatgtt ttgatattaa
     1921 caatatatcg ataaattatg ataatggcaa ttatattgtg tatggaaaag gtggtcattc
     1981 atctaaaccc gaaaaatcaa taaatcctat attagctaca attaaacttt tgtcagaaaa
     2041 tattgatgaa aaatggacaa aagatttata taagttgata aatcaagata atataaatgg
     2101 aaacttattt ggtttaaata ttgaggggaa atgtggaatt ttatcaatgg tgccaacaat
     2161 aataaatata gtagatggaa aattagaagt agttttaagc gttagatatc cagaaatatt
     2221 aaccattgaa gatataataa aaaaatttaa tttgtatatg gagcaaaata atattaataa
     2281 atttgagctt attggggaaa atttaaagca agcaaattat atagatagaa attcaaaact
     2341 tgtaagaagt ttgcatgata tatatataaa gtattctgga gatttaaaaa atgatgttag
     2401 agtaactagt gcaggaagtt atgcttctga aatgaataat tcagtaatat ttggttgtga
     2461 atttcctgat ggtagctttg gcaatgtcca tagtgcgaat gaatttgcta gcttagatag
     2521 atttataact gcaataggta tatatgcaga ggcaataatt acattatgta ataaaatata
     2581 aatacaaaat gtcccttata tagacatatt atatatacaa ttaaaatttt acttgatata
     2641 taattgaaat caaagagaag gtgatacaat gataactaag aaacatgcaa taatagtaaa
     2701 gagtttaagc aataaagatg gatatatgac atcaaacgaa ttagctatca aattggatgt
     2761 gtctactaaa actattaaaa gatatatagc agatttaaat agtgttttaa gcaaatatga
     2821 tttggtaata gcttcttcta gaggtatagg atacaagtta agcggttcta aaaacaatat
     2881 agccagagct gttaaagaag caaataaata tattgatggt tttttagatg actctgaaga
     2941 gtcaagaatg tcaaatataa tatgtatgct tattaataga aattatatga gtatagaagc
     3001 aatggcagaa gaattaaatc taagtatagc cgctataaat aaattgtcta gtaagcttaa
     3061 aagaaaactt gaaaagtatg acttagttat aaaatctaaa ccttactatg gaagctatat
     3121 acatggtgaa gaaattaata taaggcaatt aattactgac tatgcaataa aattagatga
     3181 aaaaagtaaa gtgaaggtgt ttttagatga cattagtgaa aacgatatac actgtataga
     3241 aagtatttta gaaaagcact taagagaaaa agatacaata atttcagata aagattttaa
     3301 tcttttgctt tcaaaaataa tcgtttctgt atttaggagt aagagaggtc atagtaacaa
     3361 tataaactta atggatacaa gttatagatt tcataactac tactttatag aaaatctaat
     3421 gaaagaaata tctgataaaa ttggatttaa acttattgaa gatgaagtaa tttatatttc
     3481 aaattattct ggtgtaatag cctataaagg tacacaagga agaaaaaaca caagtgaaat
     3541 tgaggaacga ataagtactg ttataagtag tgcacttcaa gatatattcc ttatttcagg
     3601 tagtgattat acaaaagatg atgagtttat ggttgcaatc tctgaccata taaagagatt
     3661 tttaaataga gctagagcta atgtaaaatc aaataatcct ttacttcatc aaataaaaga
     3721 gaagtttcct atagctttta atttagccgt gtttttatca aataaactag aaactgagtt
     3781 taatttaaaa cttgatgaag atgagcttgg atatatagct atccactttg ctgcttcaaa
     3841 tgaaagaatg aaaaaaaata ctagtaagaa aatttgtata gtttgtcatt atgggatagg
     3901 aacaggacaa cttatttctg aaaagctaaa acagaatata agtgatttaa gtgttgtggg
     3961 tgtttatcca gttagatatc tcgatatggc tataagtcaa gatgttgatt tgatagtatc
     4021 taccgttgaa ttaaaaggat atgaaaaacc tgtcttatat atagaaaata tatttgatga
     4081 tagcttaatt gaaaacgtaa acaaagcctt ttatgaaaag gaagaaagaa gaaagataat
     4141 aagcaacatg tttgatgaaa aagctttttt cagtataaag gctaatacta aagaagaggt
     4201 cataatttca ctttcaaata aactaaaaga aagagatttt atagaggaaa gttctataaa
     4261 aagtataata gatagagaaa atatttcatc aactgaaata ggaaatctag tagcaatacc
     4321 ccacacaata gtgaagggag ataaaaaatc tataatagga gttggaatac ttgaaaatcc
     4381 aataatttgg gataagcaag aggttcagct agtatttatg gtatttttta atactaaaga
     4441 aaaacataat ttctcgatat ttaaatacct ttataatttt ataaaagatg aaggcggagt
     4501 aagaggaatt attaaaatat gtgactttaa taaattaatg gcactgatag gtaattaagg
     4561 aggaaattaa taatgggaac aaacatattt aacaaagaat atgtattttt aaatgttgat
     4621 gctaagtcaa aggttgaagt tttaaaattt atatcaaaaa aagctaaaga cttaaacttg
     4681 gcagaagatg agagcttagt atatgaaggt ttaatggcta gagaagaaca atttactaca
     4741 aatttaggag aatctatagc aatacctcac actaaaaatg atgcaattga aaatccagca
     4801 gtagtagtat tgaagtttaa tgaagatgtt gtttggaatg aaggtgagga taaggttaaa
     4861 ttagcaataa gcttacttat gccaggaaaa agtaaagaga atattcatct taaattatta
     4921 tcatctcttt caagaaaatt aataaataaa gaatttaaag attctctttt aaaaagtgat
     4981 aatgttgaag aaatatctaa cttaataaat gaagctttag gtttataatt aaacactaag
     5041 gttataaaat aaaaaaataa ttaactaaaa ataggaggaa tttgaaatga ataaaaaatt
     5101 agtagcatta tgtgcttgtc caatgggact agctcacact ttcatggcag cagaagcaat
     5161 agagcaagca gcaaaggcat taggttatga agcaaaagtt gaaactcaag gtgctgatgg
     5221 tgtacaaaat gagttaacaa gagatgatat attaggagca actatgataa tacatgcagt
     5281 agcaataaca ccagaaggta tggaaagatt tgatggttgt gaagtttatg aagttgaatt
     5341 acaagaagct ataaaaaatg cagaaggtgt tataaaagaa atagaagaag atttaggaat
     5401 ataataaatt tattgtggag gaaatagaat ggcaattaag aaaaaagtta taaacagttc
     5461 aggtgcttct acaacaggag gaaataatgt aggaaaatct actccatcta gtccaactca
     5521 atcaaaggga aatggaaaat ggaaagaaat aagtaaacat ataatgacag gtatatcata
     5581 tatgatacca gttttagtaa tgggtggact tataggggcc ttatctcaat taataccata
     5641 tgctatatta ggacttgacc catctgtagg tatagttgat gctatgaact caggagaatt
     5701 tacagggttc aagcttagtc ttttaaatat agcacaatta atgtcaaatt tcggatttac
     5761 tttgtttgga tttgcgatac cactgtttgc agcattttgt gcaaactcta taggtggaaa
     5821 aacagcttta attgcaggat ttataggtgg atatatagct aataaacctg taggtgtacc
     5881 acaattcgtt gatggacaat ggactgaagt tgttccagtt gcatcaggat ttttaggtgc
     5941 aatactaata gcatttataa taggatattt tgtaaaatgg ttaaataaat ctataaaagt
     6001 ttctcataac tggttagctt ttaaaacaac atttttaata ccattaatag cgtcattagc
     6061 ttgtatggta ttgatgatat ttataataac tccatttggt ggattaatta atgaaagtat
     6121 gaaaaacttc ttaacagcag caggagcagc tggagaatat gtatatgcaa cagctttggc
     6181 agcagctaca gcattcgact taggaggacc aataaataaa gcagcaggat tcgttgcact
     6241 tggtttaact acagagaatg tacttccaat aacagcaaga acaatagcta tagttgttcc
     6301 tccaattgga ttaggattaa caacattatt agataaaaga ttagtaggaa gaagagtata
     6361 tgatagacaa ttctatcaag caggaaaaac ttctatattc ttatcattta tgggtatatc
     6421 tgaaggagca ataccatttg cacttgagag accaggtttt gttatacctt taaacatagt
     6481 tggaagtgta ataggagcta ttacaggtat aattctagga gcaattcaat ggttcccaga
     6541 gtcagctata tgggcatggc ctctagtaga taacttattt ggatatataa taggtatagc
     6601 agttggtgct atattcatag cagtaggaaa tatattctat agaaataaat taataaaaga
     6661 tggtaaactt gttgtagatt atatcgacta gtatttaatt tttactatct ataaataact
     6721 ttggctggta atatggtttt aatcaatatt accagcctta cctatatgat taattaaagg
     6781 cggtggagat aagatatgga taagatagaa aaagtaagag agtattttag agaatatgat
     6841 atagatggat ttcttataaa tagtcctaca aacaagtttt atgttggaaa tttatttagc
     6901 tcttcaggtt atgtttttat aactaaagaa tcacaatata taatagtaga ttttagatat
     6961 tttgaagaaa ttaaaagaaa atctagtttg tttaatgtag tattaatgga caaaactaga
     7021 actcattttg atataattaa tgatatttgt agagagcaga atataaagga aattggattt
     7081 gagggaaatg aagtatcttt tgatttatat agaagtatga gtaataagtt aagtgcaacc
     7141 ttaaaatcag ttgatttatc tacattaagg gaaacaaaaa atgaagacga aataaaatat
     7201 ataaaaaaag cttgtgaaat agttgatgct acattttatc atatagtaga ctttataaag
     7261 gttggtatga ctgaaaagca agttgaaaat gaaattgtta gaattataaa agatttaggt
     7321 ggtcaaaaag aatctttcga tacaatagtt gcatctgggc ttagaggagc attacctcat
     7381 ggaaaagcaa gtgaaaaagt tatagagtat ggagattttg ttacctttga ctttggtgct
     7441 aagtataata attattgttc agatattacc agaaccattt gtatgggtac tataaataaa
     7501 gagttagaag aaatatataa tatagtaaga aaagctaatg aagagtgtat aagggtttta
     7561 agaccgggaa tgactactgg agagatagat aaagttgcaa gagacataat tggttcatat
     7621 ggatatgcca ataattttgg tcataatctg ggtcatggtg taggaattat ggttcatgaa
     7681 tatcctgcat tagctcctga atcaaatgaa gtattaaaag aaggaatgat tgtaactata
     7741 gagcctggta tatatgttcc aagtcttgga ggcgtaagaa tagaagatga tgtacttata
     7801 acacaagatg gatgtatgag attgactaca tctactaagg atttaatagt tgtaagctaa
     7861 aaataaatta gataaatatt ttataacaat aagaaaagaa gaggtaaatt taaatgtata
     7921 attttgatga taaaccagat agagtaagtg aaaagtgtag aaaatgggat ttaaatatta
     7981 taagagataa atttggagat attagagaag attttatacc aatgtggatt gctgatatgg
     8041 attttaaaat accaacagaa atagaaaaca aatttattga agctgtaaga agaggtgtat
     8101 ttggatatac atactgttat gatgaatttt atgattcagt tattggttgg caaaaaaata
     8161 tgcatgaggt aaatgtagaa aaagaatgga ttacattaac ttatggaact gtatcaacac
     8221 ttcattatgt agtacaagct ttttgtaaga aaggtgatag tataatatta aatacaccag
     8281 tttatgaccc ctttgaatca tcagcaaaaa aacaaggtgt aaatgtaatt tgtaatacat
     8341 tagatgttgt aaatgataga tattatataa attttgataa attagaagtt caaattaaag
     8401 aaaataagcc taaattaatg atgttttgta caccacataa tccatctgga aggatatgga
     8461 caattgaaga aatgacaagg gtagcaacta tatgtaaaga aaataatgtt attttggttg
     8521 cagatgaagt acatgcagag catattcatt atggtacatt taattcaata cttaagattg
     8581 gaaaagagct tttagaaaat gttattctat tgacatcacc aaataaagga tttaatcttg
     8641 gtggattaaa gacttcttat tcaatagtta taaataagga tataagggac aaatttagaa
     8701 ataaattaaa gcaaaattct ataacttctc caaatgtgtt tggaataata ggattgataa
     8761 ccgcttataa tgaatgtcat gagtggttaa gtggtgtaaa tgaatatata aaatcaaatt
     8821 atgaactttt agaaacttgg gtaaataaat ataataagat aaaattgatg aaaatggaat
     8881 catcttactt agcttggatg gatataagtg gattaggtat tagtgcaagt gagtttacag
     8941 ataaattggc tatagataca ggagtattat tagaagatgg aagtcatttt gtaaaagatg
     9001 gtgagaagta tgttagaata aatttaggaa ctcaaaagga aaatgtaatt gaagccttaa
     9061 atagaatgga tttattttta aagtctttat aaaaatttgg ttgaattagt attttatata
     9121 aatttaaact attaatatgg tatatacata agt
//
LOCUS       DAEOSP010000154         8665 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.157, whole genome shotgun sequence.
ACCESSION   DAEOSP010000154 DAEOSP010000000
VERSION     DAEOSP010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8665
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.157"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            68..835
                     /locus_tag="KRH54_002779"
     CDS             68..835
                     /locus_tag="KRH54_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG8580418.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            1118..1315
                     /locus_tag="KRH54_002780"
     CDS             1118..1315
                     /locus_tag="KRH54_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG8580419.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            1344..2420
                     /gene="vorB"
                     /locus_tag="KRH54_002781"
     CDS             1344..2420
                     /gene="vorB"
                     /locus_tag="KRH54_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBG8580420.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            2423..3142
                     /locus_tag="KRH54_002782"
     CDS             2423..3142
                     /locus_tag="KRH54_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBG8580421.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            3155..3694
                     /locus_tag="KRH54_002783"
     CDS             3155..3694
                     /locus_tag="KRH54_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBG8580422.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            3851..4909
                     /gene="buk"
                     /locus_tag="KRH54_002784"
     CDS             3851..4909
                     /gene="buk"
                     /locus_tag="KRH54_002784"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBG8580423.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKDGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            4927..5820
                     /locus_tag="KRH54_002785"
     CDS             4927..5820
                     /locus_tag="KRH54_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBG8580424.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            5850..7058
                     /locus_tag="KRH54_002786"
     CDS             5850..7058
                     /locus_tag="KRH54_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBG8580425.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            7082..8341
                     /locus_tag="KRH54_002787"
     CDS             7082..8341
                     /locus_tag="KRH54_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8580426.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT         3363 a          969 c         1729 g         2604 t
ORIGIN      
        1 ttttaatata taatatttag tagagtgtgg gagtagcact taatatttgc aggaggagtt
       61 tttgtatatg ggagaaataa taaatgcatt agatagagcc ctagacataa ttttgctgtt
      121 atatcatgaa aagagagaaa tgggaataac tgaaataagt aaggctatgg gagtatataa
      181 aagtactgta catagaactt tggttactct tgaaaacaaa ggttttgtta tacaaaatgc
      241 agagaatggt aagtattggc taggaataaa tttatatgca ataggtatgg ttgtgggaga
      301 aaaaatgtca ttgactgaaa tagtaaaacc atatacgaaa aaattaaatc aagaatttaa
      361 cgaagtagtt aatgtatcta tattagaaga aagagctcaa gattctccaa gaagtatcat
      421 aatacataaa gagtatggtt caaatcaatt actttcagta aatccatccg ttggttcaag
      481 tagtgaatgt tattgttcag cagttggtaa atgtcttatg gcatttaatg attctataga
      541 ttttgaaaaa tatagaaaaa caccaataca caagtataca gaacatacaa ttgataattg
      601 ggatgatatg atgctttttt tagcaaaaat aaaagaacaa ggatatgcta tagatgatga
      661 agaactagaa catggactaa cttgtattgg agcacctatc ttagataaaa ataataaagc
      721 tattgcagcc ataagtttgt caggtccaac tataagaatg agagaagggg actttgagta
      781 taagattaaa agagttatag aaactgcaaa aagtatatct gaacttttta gatgattttt
      841 aatctcgata ataaaaatag gctatattta aattgttatt tgtaaatatc agtttaaatg
      901 tagcctattt ttctttaatc taaaattaaa tattcttaaa atttaaaatg ttattgaaat
      961 atataagcga atgtgttatc ttttaattaa gcaataaaaa tagaacaagg ttctatacta
     1021 tagaacaaaa ttgatgcata tatagtattt atacaaaata taaatattat atatttaaaa
     1081 ttaagtaaaa atattacata aaggggagat ttgagaaatg gaaaaagtca agttgagtgt
     1141 tgaaagatgt aagggatgct atctttgtat tgaagcttgt aaaaaagatg caataagtct
     1201 ttctgactac acaaataaaa aaggctttgt aactattaaa gtcgacgaag aaaaatgtat
     1261 tcaatgtggc gcttgttata caatgtgccc tgatttagta tttgagatat tataaattat
     1321 ttttattaga ggggagaaaa ggtatgcaaa gtttattaat taaaggaaat gaagctttag
     1381 cagaaggtgc tatacgtggt ggctgcagat tttatgcagg ttatccaata acaccacaat
     1441 cagaaatatt agaaatgatg gcatggagac aaaaagaagt tggaggagta tttatccaag
     1501 gtgaatcaga aatagccagt gtaaatatgg ttatgggagc aagtgcactt ggagcaagag
     1561 ctatgacttc ttcatctggt ccaggatttt cattaaaaca agaaggtatt gcatattggg
     1621 tatcagctgg tttgccagca gttgtagcat gtgtgcaaag atatggtata ggtgatggat
     1681 ttataggagc tggtcaagat aattattggc aagcagtaaa aggcggtgga aatggagatt
     1741 atcatttaat cgtgttagca cctaatagtg ttcaagaaaa tatggatatg gcatatgaat
     1801 cttttgaatt ggctgaaaaa tatatgcatc cagtacttat actttctgat gctacaatag
     1861 gtcaaatggt agaaccttgt atagcaccac caatgaaaga atacgatatg gataaagctc
     1921 catgggctgc aaaaggaaca cctgtaggag aaaaaaataa agtaataact gatataacat
     1981 attttgaaga ccaagataca tggcaaaaag ggtatataga aaaaatgaga aaagttcaag
     2041 aagatgaaca aagatgggaa gagatagaaa tagaagatgc tgaagtggta tttgtggcat
     2101 acggtatttc atcaagaata gcagaaggtg cagttagaga tgctagagca aaaggaatga
     2161 aattaggtct aataagacca aaaacattgt ggccttaccc aagaaaagca tttaagaaat
     2221 taagtgattc attgaaggga cttgtaacag ttgaaatgaa catgatggca cagatgaagg
     2281 aagatgtaat tatagcatgt gataatagat ttccagtata ttcagtagca acagctcaat
     2341 atatggcaga agtaaatcaa ttaatagatt ttgctcaaca gattatagat ggaaaagcta
     2401 agcaagagga ggtattttaa ttatggctaa aaaagcacca gcaataatga tgtgtgaaaa
     2461 tatgttctgt ggaggctgtg gacactctat agtcgataga attattggag aagtattaac
     2521 agaaatggat atatgtcaag atacaattat atgttcagat ataggttgta atcatatgtt
     2581 tcaatttagt atgaatgtag atgttgtcgt tccacctcat ggtaaaatgg gagcagcaat
     2641 ggcagctatg aaaagagtta gaccagataa atatatattt acaatagctg gagatggtgg
     2701 agcttatgca atagggcttg gagaaactat gtcagctgct actagaaatg ataatgtaat
     2761 atttcttgtt atgaacaata atatgtttgc aatgacagga gggcaaatgg ctccaactac
     2821 tatagaaggt caatatacag cttctactcc agcgggtaga aattacgcag atcatggttc
     2881 aacatttgat gttgtaaaag ttatgggaaa tttagatata gcatatctag caagaggaac
     2941 aataactaac cctaaagaaa taaatacaac aaaaagatac atcaaaaaag ctctagaaaa
     3001 acaaaaagaa aagaaaggtt tttgcttaat agaagtgctt gtaccatgtc caacaaactg
     3061 gggattaaag ccagtagatg ctatgaagag aattgaaaat gaacttgttc caaagtatcc
     3121 acttggagaa tttattgatt agggaggaaa taatatgttg aatgaaatta tatgtgctgg
     3181 ttttggagga cagggagttc ttacagcagg aaaaattata atgtatgttg catataaggc
     3241 agaaaagaaa attacatggt tcccatctta tggaaatgag atgaggggag gaagtgcaaa
     3301 ttgcaatgtt gttgtaagtg atgaaaaaat tgcaaatcct tgtgtaaaaa atccagatat
     3361 actattggga atgaacagcg tatcagtaga taagtatgaa agtttaatga aaaaaaatgg
     3421 ttatatgttt gtcaatgaat cactagtaag taaggataga aagtacagag aagacattaa
     3481 tgttataaaa gtacctgtta ctgaaatagc acaaaattta ggcaatgaaa aagctgctaa
     3541 catttgcatg ttaggtgcta tggtaaagag tttggattta tttacaaaag aagaatttga
     3601 aagtggtatg tgtgaatact ttgaaaatca aggcaaaggt aaattcaatt ctaaaaatgt
     3661 agaagcattt gaagctggat acgactacga atgaatattt taaatattta aaataattat
     3721 tgaaatactt atttttatat gttagtattt aattaataaa atatagaacg tcgttctgga
     3781 caatgggaca aagtctttag gtcggaatac aagaacgacg ttctgcatag tagaactagg
     3841 gggtgttaaa ttgaaagttt tagtaataaa tcctggtggt acatccacaa aaatttctgt
     3901 tttccaagat gaaaatgaaa ttttgaaaaa aaatataacc catacaagag aagatttaaa
     3961 aaatttttct aaagtatttg atgaatatga ttatagaaaa caactgatag tagatatttt
     4021 aagtagtgag aatcatagta taaattcttt tgacgcagta gttggtcgtg gtggacttat
     4081 gaaggctata aaaggtggta cctatactgt gagtgaagaa atgattgagg atatgagaaa
     4141 tgaaataaat ggggaacatg catcaaattt aggtgcatta ttagctaaaa ctatagctga
     4201 tgaaatagga gtacaatcat ttgtagtaga cccagtttct gtagatgaat ttgatgatgt
     4261 atcgaggatt acaggaatat cagacataga aaaagcaagt tggttacatg cattaaacca
     4321 taaagctgtt tcaagaaaaa ttgcacaaaa actaggggga aaatacgagg attataactt
     4381 tattgtagct catttaggtt caggaatatc gattgtagca cataaacaag gcaaaatgat
     4441 tgatggaagt ggtggtaggt ctgatggtcc attttctcca gaaaggagtg gtggattact
     4501 tgcatatcct cttatagaat tgtgttactc cggaaaatat actcatgatg aaatggtcgc
     4561 aaaagtaagt tcgattgggg gaatgtacga ttatttagga acaaaggaca tgatagaaat
     4621 tgaaaacaga atcaaggatg gtgatgaaaa ggcaaaactt ataatggatg catttattta
     4681 tcaagtagca aaagaaattt ctatgtatgg agcttcactt tcagggaatg tagatagagt
     4741 tatcttgaca ggagggatat ctcactcaga aatggtagtc aaaggagtag cagaaaaagt
     4801 ttcatatcta gctccaatag agatagtacc aggagagatg gaaatggagg cacttgctct
     4861 aggggcactt agagtactta atggagaaga aatagcaaaa acttattaat atttaggaga
     4921 aagagtatgt taaaagattt taataattta ttagaagagg ctaaaaagct tgaacctgtt
     4981 attgtaagtg ttgctgttgc agaagacaaa gaagtattaa aggctgtgaa atcagcaaca
     5041 gatttaaaca ttataaagcc aattcttgta ggagacaagg ataaaataga aagtatatca
     5101 agagaaattg gattaaaagg tcatgaaata ataggttgtc aaaatcccga tgaatcagtt
     5161 aaaaaagctg tagagatagt taaaaataag gaagctaaag ttcttatgaa gggattagta
     5221 aatacaagtg tctatatgag agctatatta aataaagaaa cgggtcttag aacaggaaga
     5281 ttattaagtt tgcttgcagt atatgaatta cctcaatatc ataaacttct ttattgtact
     5341 gatagtggaa taaatgtatc gccaaactta aatcaaaaaa aagatataat gacaaatgca
     5401 cttcttacta tgaaaagtat aggcatggaa aatcctaaaa ctgcgatact tactgcaaat
     5461 gaaatggtag accctaagat acaatctaca gtggatgcca acctacttgt agagatggaa
     5521 aaatcgggag aaataccaaa gtgtatagtg gaaggtccaa tagcatttga tgtagcattt
     5581 gatgcacatg cagcagaaca taaaggtatt aaaagtaata ttgctggtga tgttgattta
     5641 ttagtatttc caaatataga agcagggaat atattaggaa agtcatggct tagatttaat
     5701 aaagctaagt gggcaggtat agttttagga gcaacaaatc cgataatttt aggttcacgt
     5761 tctgacacag ctgaaataaa aataaattca atagcattag cgtgcctagc atcacaataa
     5821 gaaattatta ttaaggagga aaaagtatta tgaaatatgc aaaaagaatg gatatggtaa
     5881 aagcttcagc tataagagat tctcaaaaga aaatagctca gaaggtagca agtggtggaa
     5941 ctgttatatc ttttgctgca gggcttcctg acccaaattt atttccaata gaagagataa
     6001 gtgaaataac tcaaaaagta cttaatgaaa ggggaaaatt tgccctagca tatggaccta
     6061 ctaaaggtga agatgagttg ttagatctat tagtaaaaag aatgaaagaa gaagaaaata
     6121 ttgatacaaa agcagaaaat attataataa ctactgggtc acaacaaggg atagcattaa
     6181 gtgctatgat tttattagaa aagggagatg tagtagttac agaaaatcca agttacttag
     6241 gtgcaataaa tgcatttaga ccttatgaat gtgatttttt aggagtagat acagatgaag
     6301 aaggaatggt aacagatgat ttagacaaat tattatctga aaattctaac ataaaggtga
     6361 tatatgtaat accaacattt caaaacccta caggaaaaac atggtcttta caaagaagaa
     6421 aagattttat ggaagtcgtg aacaaatatg atgtcatagt aatagaggat aatccttatg
     6481 gagaaataag atttacacca gaacctctac ctacacttaa atctcttgat actaaagata
     6541 aagtattgta ccttgggtca ttttcaaagg tattatgtcc aggatttaga gttgcttgga
     6601 tgtgtggtaa tgtagacatc atagataagg cagaactttt aaaacaaggt atagatttac
     6661 agtcaaatca attttcgcaa gtacaagtaa tagaattttt aaagaaatac aacttaaatg
     6721 aacatataga aaaaataaga gcagagtata agaaaagatg tctgctaatg ttagatgcta
     6781 tgaagaaaca tttcccagag gaagctaaat atacagaacc agatggagga atgtttatat
     6841 gggttgaact tcctgaaagt ataaatgtag atgatttatt agataaagcc attgatgctg
     6901 gagttgctta tgtttctgga gagtccttct ttgcaaataa tggtccaaaa aatactatga
     6961 gacttaacta tacaacaatg tcagaagaac aaatcgttaa aggtgttgag atacttgcag
     7021 aagttataaa aaaagaactc aattatgctt gtgaataaat ataaaaagtg agggtttaaa
     7081 tatgggagaa aaagaaaacg gtataaagtt tggaaagtta atgtttcaac ttgttattat
     7141 agcaatatct tgggaattag tttatattat accatttatc caatatacat tatatgatcc
     7201 aatcctaaaa gcattagaat gtacaaacac tcaactaggt tttctactta caatttatgg
     7261 gttaggtaat atatttggag ctcctatagg aggctggtta gcagatagat ttgattatag
     7321 aaagataatt ttaggttctg tatttcttaa tggagtagta tcatttttat ttgcatttaa
     7381 tatgaactat ccatttgcag ttgctacttg ggtaggttgt gcaatcacat cattagttat
     7441 gaactatcct tctatggtaa aaatacttag agttatagga aaagataatc aaggtaaggt
     7501 ttatggattt aatgaagcta tggttggagt ttctggagta gtaatgggag caatattctt
     7561 atatatttat acatgctttg caactcctac attgggtatg agatgggtaa tgatatcttt
     7621 aggaatatta tcaatagcaa tgtgtccagt attatggttt gtaattaaag atgtagatat
     7681 taaagaagaa agagaagaag caggtgaaaa aatgtctgct ggtgatttta taacagtact
     7741 aaaatcacca aatacttggc tagtaggaat aagtatattc tgtgtatatt cattcacagt
     7801 tacaatgtct tattttacac catatataac atctgtactt ggaggttctg tagcattatc
     7861 tggtgcatta gcaataataa gacagcatgg tctaaaatta tttggagcac catttggagg
     7921 atattgtgca gacaaattaa aatctcctac aaaagtactt ttaccaatat atgtatttgg
     7981 tatagcagta atagttttat tcctagtatt acctgcatca actccaatga ctatattcat
     8041 agctttaaca tttgtagtag gtatattagg ttacatggga aaaggtatat attatgcagt
     8101 acaagatgaa gtaaaagttc cagttaaata ttcagcaact actataggta tagctgcagc
     8161 tttaggattt tcaccagatg tattccaatt tgcattaata ggacactgga tagatactta
     8221 tggcaataaa ggttacacat atacatttat attccaaata gcaattctag ttattggaat
     8281 actatcttgt ttatatatat taaaggttaa aaagagaaga gcaagtaaat tagaagctta
     8341 aatattcaat ttggtaatta agtatatata aatacaatac ttattttgat gaatgtgatt
     8401 gtataaataa cataaaaaat atcaatagat taagttttat aataatgttt agagaataaa
     8461 gtataaaaat caaaatgtta tcaagttttc taggttttaa cataaagtta cccctgaagg
     8521 tgtaaaattg agtggcaatt attatctatt ttcttaccta cataaaccta tttaatttaa
     8581 attctaagag attttcaaaa tagatggtag ttgtactcaa ttttaaacct cataataaat
     8641 acgaaaattt aaaatctaac tagat
//
LOCUS       DAEOSP010000155         8544 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.158, whole genome shotgun sequence.
ACCESSION   DAEOSP010000155 DAEOSP010000000
VERSION     DAEOSP010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.158"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            289..1275
                     /locus_tag="KRH54_002788"
     CDS             289..1275
                     /locus_tag="KRH54_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBG8580427.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKKLKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     EMSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKSIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1444..1592
                     /locus_tag="KRH54_002789"
                     /pseudo
     CDS             1444..1592
                     /locus_tag="KRH54_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            1611..3092
                     /locus_tag="KRH54_002790"
     CDS             1611..3092
                     /locus_tag="KRH54_002790"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBG8580428.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            3118..4089
                     /locus_tag="KRH54_002791"
     CDS             3118..4089
                     /locus_tag="KRH54_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBG8580429.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4345..4947)
                     /locus_tag="KRH54_002792"
     CDS             complement(4345..4947)
                     /locus_tag="KRH54_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG8580430.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKSVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILSNSIELDNC"
     gene            5228..6439
                     /locus_tag="KRH54_002793"
     CDS             5228..6439
                     /locus_tag="KRH54_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBG8580431.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSKCGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            6507..6704
                     /locus_tag="KRH54_002794"
     CDS             6507..6704
                     /locus_tag="KRH54_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBG8580432.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            6738..7709
                     /locus_tag="KRH54_002795"
     CDS             6738..7709
                     /locus_tag="KRH54_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8580433.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFRNALWMYKFTGLFIKDKTK"
     gene            7896..8273
                     /locus_tag="KRH54_002796"
     CDS             7896..8273
                     /locus_tag="KRH54_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8580434.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         3476 a          890 c         1368 g         2810 t
ORIGIN      
        1 taattaagtg tttatcgata aggctatggt tgtaaaaatc atagccttat ttttatatta
       61 taaataaatt agagtattta tcaattcata gtagataaaa ttgaaattaa atataacaac
      121 aaaaaatgaa aattacaatt aatttaatgt taaagactca attatttgga tgtggaatgg
      181 atagtacaag tagtatataa aaagtattga ctggacaaat aactatattt tattttaaat
      241 atataatata atgtaataaa atatatcaaa tgtagaaggt gataacctat gaaaaaaata
      301 acaataaatg atattgctaa cttggcagga gtatctaaaa gtactgtatc aagatatcta
      361 aacaataaag atatcagtga ttctactaaa gagaaaataa agacaataat agatgaatat
      421 ggatatgagc cgaatgcatt tgctcaaagt ttgagagcta aaaaaacata ttttattggt
      481 attataacac cgtgtttaga ttcttttgta aaatctaaaa ttatgatggc aatagatgaa
      541 gaactcaaaa aattaaaata tacatcacta attattaata ctaatagaaa aataaggtca
      601 gaaatagata gtatatcaaa gttagctagt ctaaaagttg atggaataat actaattggt
      661 accgaaataa ctaaagaaca taagaatgaa attgaaaagt tagatatacc gatagttgta
      721 gttggacaaa aagttgatgg tatcaatagc atagtgaatg atgattatgg agcaggctat
      781 aaaatgggtc aatatattgc taataaaggt tacaaaaaca ttgtatattt aggtgttgat
      841 gaaagtgata tatcagttgg tttaaataga aaaaatggag tacttaatgg gttaaaagat
      901 aaagggtatg atgcaaaagt attttatact gactttgacc aagaaatgtc tatacaaaga
      961 agtggagaaa tgctagaaag tgaaaatcca gatataataa tatgtgcaac tgataatata
     1021 gcaatagcaa caatgaaaga aatcaataag agaggtaaaa gtataccaca agatatatct
     1081 gtagctggat ttgggggata tgatgtattg tctattataa gccctaaact tacaacaata
     1141 aagtttgaaa ataaaaatgc aggtaaagta gcagcaaata cgatagtaaa tttaatacaa
     1201 gaaagaaaag aaccactatt aaaggagatt aaatttgaat taatagaagg tgaaagtaca
     1261 ataaataaaa attaagatac acattacata tattagtaat gtgtattttt ttactaaaaa
     1321 aactataatt ctaaaaatta ggaaaacttt tttgaaagaa atattgcatc tgaaataaat
     1381 ttatgctaat ctttaattaa accggtttcg aaaccggttt aattaaaccg atagaatagc
     1441 aaaatgagtg caaaaataac tgattgtaac aaagtatcaa agtaattatt ggagtacata
     1501 ggaataaaca aaatataaaa gatgtagcac attgtgtaat aagactaaga attgttttag
     1561 atagcaataa taaagcaaat ataaaattat aaaagaaaag gaataaaaat atgtacaaag
     1621 agcctaaata tagaaccata ttagaatcga caaaagaaga attaaataaa ttaagaaata
     1681 tatctttaaa tgataaatat aaaccactat ttcatataca tccacaacat ggactactta
     1741 atgacccaaa tggattagca tattataatg gtaagtacca tgtattttat caatggtatc
     1801 catatgatgc aactcatgga atgaagcatt gggcttatgt aagttctgat gattttgtaa
     1861 actggaatag agaagatgta gcattaatac caatcgaaag ttatgaatct catggagcat
     1921 attcaggtaa tgcgatagag gtagatggaa aattgcatat gtattataca gggaatataa
     1981 aatatagtgc agaagataga tatgcatatc aaaacttagc tataatgaac aaggatggta
     2041 aaataactaa atatgaaaat aatccaatag taagtgaaat accaaaagga tatacaggac
     2101 atgtaagaga cccaaaagta tttaaaagaa aggataaata ctttatgtta cttggagcac
     2161 aaacaagtga taaaaaagga gttataatag tatatgaatc taaaaactct atagattgga
     2221 attttaaagg agaattaaat gtaaaaaaca tagatgaaga ttttggttac atgtgggaat
     2281 gtccagatta tattaatata gatgaaaaag atatattaat attttcacca caaggagtag
     2341 aacctaaagg atttgattat caaaatatat ataatgtagt atatgctatt ggtaatatgg
     2401 atttagataa tttaacattt gaaatagata caatgaaaga actggaaaaa ggatttgatt
     2461 tctatgctcc acaaacattt ataaaggact cacaaataat actatttgca tgggctggaa
     2521 tgggagaagt tttataccct acagataaaa acaaatgggc acattgtcta actgttccaa
     2581 gaaaattaaa tataaaaaat aataagctat tgcaaatgcc agtagatgaa ttaattaaat
     2641 taagatatga tgaaacaagt gggcaaaata ctataaagaa taatattaat attatagaaa
     2701 atgatgaaaa tgtatatgag cttaatatta atataaaaaa tattgacagc aataaatttg
     2761 ggctggaatt attttcatca caagatgaag gtgtaaaatt agaatttaac aaattaggaa
     2821 acattgtaac tttagacaga agcaacttta aaaaagtttt cggtgttgaa tatggaacaa
     2881 atagaaaaga atatataaat attgatgaaa atactaatat caaagtacta gcggatagaa
     2941 gtattttaga aatttttata aatgaaggag aagtagtatt tactagtaga atatttgcta
     3001 aagaaaattc taatcaaata agggtttata gtgataaaat agtagagtat gagtatacta
     3061 aatttaaatt aaagcaaggt attgaattat aaaaacacat tataaggggt agaaaagatg
     3121 aaaaaagtaa ttagtatagg agaagcttta atagacttta tacctaatca aaatggtggt
     3181 aaattaaaaa atgtatcaga atttagaaga gtagcaggag gagctccagc aaatgttagt
     3241 gctgttgtag caaagcttgg aggaaaatcg agttttatat ctaagttagg aaaagatgca
     3301 tttggtgatt atataataga tgtattaaat gaagtaaatg taaatactga ttatgtattg
     3361 agaacaagta aagcaaatac aggacttgct tttgtatcat taaaagaaga tggtaatcgt
     3421 gatttctcat tttatagaaa tccaagtgca gatatgcttt tagaagctga tgaagttaaa
     3481 aaagagtggt ttaataattg tcatatccta catttttgtt cagtagattt aatagatagt
     3541 ccaatgaaat tagcacataa aaaagcaata gaatacgcac ttgaaagtaa tagtataata
     3601 agctttgacc ctaatataag attaccttta tgggatagtg agcaatcttg taaaaaggca
     3661 atatcagaat ttttaccttt tgctcatatt gttaagatat ctgatgaaga attagaattt
     3721 ataacaggag aaaatgatat agaaaaatca ttacataaac tctttgtggg gaatgttgaa
     3781 ctagtattat atacaaaagg aaaagatgga gtagatgcat acactaaaaa agtaaaaggt
     3841 atgtgtaaag gggttaaagt aaatgcaata gatacaaccg gagcaggaga ttcttatata
     3901 ggttcatttt tatacacctt attgtataaa caaataacat tgaatgatat aaaagaaatg
     3961 aaacaagaaa cgttaaatga gtatttagaa ttttctaatt attatgcagc tcgtagtaca
     4021 actattaaag gtgcgataag ttcatatgca acaaaggaag aaataactga atttataaaa
     4081 aatttatagt aggaatgagt aataggtttt gaaggaagta acatgtaaag agtttttaag
     4141 gatgtttact atgaaaataa ttaacatata aaaagcaata aggaggctgt ctcaaaatag
     4201 agataagcct ctttatcatt gttgaaatga taaaaaatat tttaattatt gttacttttt
     4261 agaagaatat atatcttatt ttaaataagc aaattatgta agatttattt atattatcaa
     4321 tactttactt ggagtattat gtctttagca gttatctaat tcaattgaat tagataaaat
     4381 ataataaatt tgttttgcaa attgacgaga atgttcttct ttttctttat tcataaaatt
     4441 aggtttacct aaatcacgtt caacaagcat tcctatatat tttaactgtg aatgcttaca
     4501 gtagcgcttc ataccatttt cccaaatatc agcacccttt tcaggtctat atccacatgt
     4561 tgtaataagt gctactgatt ttcctgccca tagagcgggg cctttctcat taccatagta
     4621 tttattcatt ccatacacca atctgtcaag tagtgctttc attggagaag tgcagtacca
     4681 agaataaatt ggagtagcaa aaacaattaa atcacacata agaattttct tgaaaatttt
     4741 ctgaacatca tctttacaat aacatccaaa atcaaataag ttatcctgac actttttaca
     4801 agcaatacat ggctctattt tacaatcata taaccatatt acttcaatat ttgaattata
     4861 attttctaat tcctcaataa aaggttttaa aatagcagca gtattacctt ttttcttagg
     4921 acttcccata agaatgcata cttccatatt gatacctcca cagtatttta aaatagtttt
     4981 tagtgcttaa tatagtttta ggatactatg agaatacgtt aacaacaata cactttgtat
     5041 atttgtaata aaattgaaac aaattgtttt atcaataaaa gttggaatgt atctataatt
     5101 aaaaatattt taggtatgtt tttacatata tgtgtgtagt taactatggt aatttattgt
     5161 aaaattatct tataggatta taattgctta atttatgatt atatttaaaa tcttggaggt
     5221 aaaaaatatg tactcaaatg aagactataa atttatagaa gaaaatgcac catgttatat
     5281 atatgatgag actaatataa ttgaaaaatg taaggaattg aaaaataata ttttaggttt
     5341 tgatttttta tattctataa aaacaaatcc atataacaaa attgtaaaaa gcattgcaga
     5401 gcaaggtttt ggagcagatg cttcttcaac taatgaagtt atgatttcat caaaatgtgg
     5461 agtttctaaa gatgatattt attattcatc accaggcaaa acagagattg atatagaaac
     5521 tgttttagat aaatgcataa ttattgcaga tagtattaca gaaatagaac ttataaataa
     5581 gatatctaaa aagcataatc agaaagttaa aataggaata agaataaatc ctgattttac
     5641 tatggatgat gatagtgcta aatcaagcaa atttggtata gatatagaag atatgagcag
     5701 tgtttttaag atgttaagta tgtgtgatta tgtatctgta gttgggattc atatacatat
     5761 taaatctcag gtattggatt ttgaaaagtt aggacattat tatgaaaaat gtttttttat
     5821 agctttaaat ttaaataaaa gtaagtatat taatattgaa tttataaatt ttggtagtgg
     5881 acttggagta gcttatgata atcttagaga aaatccactt aatcttaaag agctttcaaa
     5941 attaactcaa aagattgctt ttactaattc aaacacacta aaatcaagac ttcttattga
     6001 atcaggacgt ttcatagttt gtaaatcagg taattattat gttaaaattg tagatattaa
     6061 agtatcaaga gggaaaaaat atcttattgt aaaaaatgga cttaatggat ttatgagacc
     6121 tattatttct aatttaattg aaaatattac ttgtggagta gaattagaag gtcaagagcc
     6181 attatataca tcaaatcatg ccttttctct ttcagtatta aataatttaa aagaagaaga
     6241 gtatgtaaat gttgttggga atctctgtac atcattagat gtaattgcaa ataatgttaa
     6301 attgaaaaaa gcaaacatag gagatttgat ttcaattagt aatgctggaa gttactccta
     6361 ttctttatca ccgctattat tttcaagtta tgaatttcct aaacaatatt ataaaagaaa
     6421 agatggatat attgagtaaa caatagcata aattaataaa cttagtatta ttgaaaaaat
     6481 ttaaaatgca gggggtgtaa ttctatatgg aattgtggaa aagaaattta tttgtatgtt
     6541 ggataggtat gttttttagt tctataggga tgagccaaat agcacctata cttccattat
     6601 atattaaaca gttgggggta actgatgtta gtttaataca acagtattca ggtataattt
     6661 ttggatgtac ctttgtagta gcagcattct tttcgccaat ttgagggaaa gctgctgata
     6721 aatatggaag aaaatcaatg cttttacgtg caagtattgg gatgggaata gttatattta
     6781 ccatggcgtt tgttcaaaat gtttatcagt tattaggatt aagaatactt caaggagttt
     6841 ttacgggtta tgctactgca tgtactactc ttattgcaac acagacagat aaaaatcatt
     6901 caggatgggc tttgggtact cttgcaactg catcaactac tggctcatta attggtccaa
     6961 cagtaggagg atatatagaa tcaatcttag gtcttaaaag tacatttatt ataacaggag
     7021 gattgttgtt tgtatcattt atcatctcta ttttatttgt agtagataat tttaaaccta
     7081 aagaaataaa agaatcaatt tctataccta aagaacaatt aaatatattg ccaaataaat
     7141 ttttaattgc atctattttt gtaaccacat tcattactca actagcttta tactctatag
     7201 aaccaattgt aaccatatat atatcacaac ttacaaattt tgcatcaaat gtagctttaa
     7261 tagctggatt gacattttct gcatcaggat tagcaaattt attagcagct caaaaacttg
     7321 gaaaaatgtc ggataaaata ggtcctcaaa aagtattgtt aatttcactt ttatgggctg
     7381 ggattatatt tataccacaa gcttttgtaa aaactgcatg gcaactgatg ttgttgagat
     7441 ttttattagg gttgtcagtt gcagggctta atccttctgt taacagcttg cttaaaaaaa
     7501 ttgcaccaga agaatatgtg ggaaaaatat ttggatataa tgcatctgcc cagtatatag
     7561 gctgttcatc aggagcattt ttagggggac aaatatcagc acatttaggt attagaacag
     7621 tatttttttc aacaagttta ttgttgttta ggaatgcact ttggatgtat aaatttactg
     7681 gattgtttat taaagataag actaaataat catactattt acatttctaa ttttatgatg
     7741 taaaataaat attttttatg taatttatca tagaattata taaaaaataa ccctatggta
     7801 aaaattggaa aatattatta tcgacaaaat ctttgactta aaaactatat tgcgatagta
     7861 ttaatttaat aaaagtttga gaggggcgta aggttatgac aatttcaaaa ataccacaag
     7921 cagaattaaa agttatgaag tttatttggg aaaaaaatga tacagtaaca tcaaaagaaa
     7981 ctattgaagc tatggaacga aaatatggtt ggaaacaaac aactacattg acacttttat
     8041 caagacttgt taaaaaacat tttttaagtg cagaaaaaat agatagatac actcattata
     8101 caatagttgt tggacataag gagtatttaa gtgttgagac aaaagatttt ttaagcaata
     8161 tacacgacaa ttctttacag agtttaattt cagcactaca tgatgatgaa gttgttgata
     8221 aagatatgtt ggatttattt gaaagtcatt ttaaaaattt agaagaagag taattatatc
     8281 aggatagaac tctaagaatt taattaatct atggctgaaa accataaatg gtattattta
     8341 atcagctact cactattcat aaagtaatat taaataggtt ctctaattag taaaatgcct
     8401 aaaataattg gaatagggag tatcaagtta aatgatacca cttatttaaa taagaaatga
     8461 tttttaaaat ttaaaataat tttataactt gtcaatattc attaatctaa agtattattt
     8521 ctatttaacc atatcaaaat atat
//
LOCUS       DAEOSP010000156         8524 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.159, whole genome shotgun sequence.
ACCESSION   DAEOSP010000156 DAEOSP010000000
VERSION     DAEOSP010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8524
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.159"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(34..1710)
                     /gene="bclA2"
                     /locus_tag="KRH54_002797"
     CDS             complement(34..1710)
                     /gene="bclA2"
                     /locus_tag="KRH54_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HBG8580435.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGPTGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGAVGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLATNNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(2088..2627)
                     /gene="hpt"
                     /locus_tag="KRH54_002798"
     CDS             complement(2088..2627)
                     /gene="hpt"
                     /locus_tag="KRH54_002798"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBG8580436.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGRVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(2955..4262)
                     /locus_tag="KRH54_002799"
     CDS             complement(2955..4262)
                     /locus_tag="KRH54_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBG8580437.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEGIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4687..6462)
                     /locus_tag="KRH54_002800"
     CDS             complement(4687..6462)
                     /locus_tag="KRH54_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBG8580438.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6459..6596)
                     /locus_tag="KRH54_002801"
     CDS             complement(6459..6596)
                     /locus_tag="KRH54_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580439.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6674..7243)
                     /locus_tag="KRH54_002802"
     CDS             complement(6674..7243)
                     /locus_tag="KRH54_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG8580440.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7427..8062)
                     /locus_tag="KRH54_002803"
     CDS             complement(7427..8062)
                     /locus_tag="KRH54_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG8580441.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         2455 a         1678 c          967 g         3424 t
ORIGIN      
        1 gtttattatt ttataatctt ttcatcatat aaactattgt attctataaa ctgatacata
       61 tccagctgca gaagtacctg tcgtgcctga accaaatgta aaacttaaag taacagctgc
      121 tgctctagca tcaactaaaa agcttaaact tacacttaca tctgatgaag taagtgtatt
      181 tactgctttt gcaataaagt tattttgagc tactccatta aagttagtag taattgacat
      241 aaatcctgca agtgaaagtg tggcacgtat accgaaagat acattgtata tccctccaac
      301 tgcaagattt atagtactac ttgttggaaa agttatatta gttccattta taaatgatga
      361 attaaatgtc actaatgtat tattcactaa acttgaagag gacactgtaa attgtgcatt
      421 gtttgttgct aacactcctg ttgctcctgt cgctccggtt ggacctgttg ctccatttgc
      481 tcctgttgct ccggtcgccc ctgttgctcc tgtcgctcca tttgctccag ttgggcctgt
      541 tggtcctatc gctcctgcta ctccatttgc tcccgttgct cctactgctc ctgttgggcc
      601 tgttgctcca tttgcccctg ttgctcctgt cgctcctgtt gctcctgcta ctccatttgc
      661 tccggttgga ccggttggac ctattggacc tgctactcca ttcgctccgg ttgctcctac
      721 tgctcccgtt gggcctgttg ctccatttgc ccctgttgct cctgtcgctc ctgttggacc
      781 tactgctcca tctgctcccg ttgggcctgt tgctcctact gctcctgttg gacctattgc
      841 cccagctact cctgttgctc ctgtcgctcc tgttggacct accaaaccat ttgctccggt
      901 tgctcctact gctcctgttg gacctaccaa accatttgct ccggttgctc cagttgggcc
      961 tgttgggcct gttggaccta ccaaaccatc tggacctgtt gctcctactg ctcctgttgg
     1021 acctacctct ccatctgctc cggttggacc tgttgctcct attgggcctg ttgccccagt
     1081 tgttccggtt gctccagtcg ctcctatact tccggttgga cctgttgctc ctgtatttcc
     1141 tgttggtcct gtcgctcctg ttactccatt tgctccggtt gctcctgtat ttcctgttgg
     1201 acctgttatt ccatttgctc cggttgctcc ggttgctcca gttagtccgg ttggacctgt
     1261 tgctccagtt gctcctgtat tccctgttgg tcctgttatt ccatttgctc cagtcgctcc
     1321 tgtatttcct gttggtcctg tagagcctgt tattccattt gctccagttg ctcctttgtt
     1381 tcctgttggt cctgttattc catttgctcc ggttgctcct gtattccctg tcggacctgt
     1441 tattccattc gctccagtcg ctcctgtatt tcctgttggt cctgtagaac ctgttgttcc
     1501 atttggtcca gtcgctccca tatttcctgt tggacccgtt attccattcg ctccggttgc
     1561 tcccgtattt cctgttggac ctgttattcc atttgctccg gtcgctccag ttgcacctct
     1621 aggccccgtc ggtcctgttg gaccagtagc acctgttggc cctgttggac ctacatcttg
     1681 atataaactt ggacctgaaa tatcactcat ataattaatc ctcctttttt aaagttagag
     1741 tattactcta taaatattga gtattactct atataattat atgaatcgac aatttcttat
     1801 gtgataatgt atctatttgc ttatatcaac atatatgctt tgattcttga atttcattac
     1861 ttttttgtaa aatcttttac aattattagt actcttttac aattattaat acaattaaaa
     1921 aactcatttc ccatcaatta tttaaaattt ttttattatg ttataaaaaa tagataatta
     1981 aattgcataa aattaattta atactacttt aattactttt taaataaaaa aacctcaaca
     2041 cgattaataa aaatcatatt aaggcatcca caaaattata taattgctta tctatcttca
     2101 tcagtaacat tgaatatata aggaacattt ctataataat caccaaaatc aaacccatat
     2161 ccaaccacaa acttatcttc tatttcaaat ccacaataat ctggcactaa atcgactttt
     2221 cttctgcttg gtttatccaa aagaacacag cattttaaac tagcaggatt tttagctttt
     2281 agatgtttta acacaaaatc catagtaagt gcagaatcag taatatcgtc tacaaccaaa
     2341 acatcatatc ctgctatatc agtagtcaca tctgaaacta ctttaaccct tccagtactt
     2401 tcttcattgt ttccataact agatgtagtc ataaaattaa ctctaagtgg taaatttata
     2461 tttctaacta agtctgcaca aaatataaag ctacctttta atagagaaac taccataaga
     2521 tttttatctt tataatcttt ttctataatt gctcctaatt cccttaattt atctttaatt
     2581 ttttcttctg aatacaatac ttcccatttt tttgtttcta tatccataaa aagacctcca
     2641 attgtaataa tccttcttaa ttttataaga gattgctcac tttttcaata ctcttattca
     2701 atattagtca atattattga gtttttatgt tcattcaaca attattaaca atgtatctta
     2761 tattttaagt agttcatata tccacactaa attacatact atatatttta gacaaaaaaa
     2821 gaaaaaactt gcactaacaa gttttttcta ttatacataa atttatacat aatcatattt
     2881 tattagaggg tactaattaa atttatttat gctatttttg ttatacctca taatacaaaa
     2941 catattataa agtactagtc aactttattc atatcattca ttacatttaa gattatctca
     3001 tctgttatag acataccttt gtcacttact ctacctaagt tttgtatact ttgctcaaca
     3061 cttgaaccaa ctataccatt tagtggtggt acaaaacaat cttgcttagc tattatagca
     3121 ctttgtatag ctgcagatgc tgcagaagat aattttaatg cacaaccagc tttcgcaccg
     3181 tcacatatca ttccacttaa gtcagctatc atattttcta tagctccttc tatttgcttt
     3241 tcagtaccat tcataagcca agagatacca gcagttgctc cagtagaagc agcaacacca
     3301 catccacata cagcagataa tcttcctgta tagttcttaa tataaccagt tactagatga
     3361 gatatagcta aagcttttgc tagtctttca tcagattgtg ggaatttttt attataagca
     3421 acgattggaa gaattgctgt taatccatga tttccacttc catttgaact cataacaggc
     3481 atttttaccc ctgccattct tgcatctgaa gctccagcag ttaacatcat tgcataattt
     3541 ataacatcat tacctaaaag accttcttct atagactttt taattccata tcctacacca
     3601 ataccagttt tttgttttaa tccatattca gccatctctt cattcatttt tactccatct
     3661 agtaaaaatt ctatatcttt aaaatcaatt ccttctacat tttttattaa ttcttgtata
     3721 gttactgtat ccattaaact ttcagctttt tctgccgcat catttgaagc tgatactttt
     3781 ggttcattgt ctaataatac ttctccatct ttttctaaga acgtaaaatt atcatgttta
     3841 gttcttattc tcactttagc aatatgattt tttcctttta acacaacttc tataaatact
     3901 ttctctcttg tatcagcagg tgttattgat agtggagtat tatccatata atcctcagct
     3961 attttaactt cctcttttgt tagagtttct aacacactta atccattttc agaatgtcca
     4021 cctactatac ctagagctgc tgcaattttt aatcctaatc tttcagtacc tggtatacct
     4081 acgcacatac catttttata aatactagga ctaactaaca tacgattttc aactatttct
     4141 tctcctaata attcctttgc ctttgcacat gctagagcta aggctactgg ttcagtacac
     4201 cctactgctg gtttaacttc tgcctttaac atttccacta attcttttct tatatctctc
     4261 attatatata aacctcccaa ttaagtttag atttagtact caaataatta attaaataat
     4321 ttcttatcta tatttatagc aaaatacatg ccatattttt tatatctttt tatattttta
     4381 gtcttatatt ctataaccat tggtatgact gcttctattt atgtattttt tttatttaca
     4441 tagagtattt atattcttta aaatggagaa acatatgaca atttcgcaaa aagacaaaaa
     4501 aaagtctcaa aatgagattc tatatcattt tgagacggct caaatgtatt attttttaac
     4561 ttataaaaac cattttttaa ttcataaaaa aagattctgc aaaacactta tagtataagt
     4621 ttaaagcttt ctttaaagtt atgaatttag aaaaatttaa attttatata gctttttatt
     4681 atattactat gaaattatat tgtatttttc aatttttcta tataatgtag ctcttgatat
     4741 tccaagtgct ttagctgtta attctttatc ttttttataa tctttgtact tatttaaagc
     4801 ttttattatc tcaatcttct ctagttcatc aagtggaatt atcctatcgc ttttaacttc
     4861 taactcttct acagaggtat tcttaatttt ttgaggaatt acattcattg tgattattga
     4921 agaagaagac atattcacac tatattcaat aatattctgc aactctctta tattccctgg
     4981 ccacttatat tccatcatag catcaacaac cctgctatct atgtcaataa catttttatc
     5041 taattttttt gaatactctt taaccatatg cttgattata agtgggatat cttcttttct
     5101 ctctcttaaa cttggtaatt ttataggtat aacatttaat ctatagtata aatcttctct
     5161 aaaagtgcca ttttgaacca ttttttctaa atctttattt gtagcagcaa taattcttac
     5221 atctattgaa acatttgatt tacttccaat tttattaagt tccttttctt gtaatactct
     5281 caataacttt gactgtaaat gcaaactcat atctcctatt tcatctaaaa atatacttcc
     5341 cttatgagct acttcaaaca ttccaagctt tccacctttt ttagctccag taaaagctcc
     5401 ctcttcatat ccaaataatt cactctctaa taaattgtct ggtatagcag cacagtttac
     5461 agctataaaa ggattatcta ctctattgct atggttgtgt atggctcttg caaatagctc
     5521 cttacccgtt ccactttccc cagtaataag tactgttgat gttgatttag aagctattag
     5581 ggcttccttt ttagaatttc ttatagctac actttctcca attatgtttt ccaattttat
     5641 tctataatct ttattcatct tattgtagtt ttttatagca tctctcttat ctataaaatc
     5701 aataacatat ccctttaact cattttttaa tactatttta ttgatgttgt atattccctt
     5761 cgaatcatat ccgtatcttt tataaaaaaa actacaggat ttgtccttct taaagttatt
     5821 tatagaaggt ttctttataa aatctaattc gacaaatatg tttttgccag ttatactatc
     5881 ttttatattg aaaagctctt taaattttga attatattta tcaatttttc cgtccacatc
     5941 aatagacaca acagctttgt ccatattgtt taatagtgtc tctaattttt tcttttctaa
     6001 ttcaagctca taagttttaa tttgtgcttt taatttatta gatattaggt ctgccatttt
     6061 gcctaaaaag tttataagac cacttttatt attttctata atttgtgctt gttcttttgt
     6121 aaaggctata aggcctataa caccatgaga atcgccatca catataatag gacaacacac
     6181 ctctgcaaac tctacacaag ccccactttt atagcattcc ctacatatat cactactctt
     6241 tgggtcatca attagtattg taatctgttc tgtaagtgat tttttaaaag ccgtatattt
     6301 atctactttc tcacctattt tgtctatata tactcctgta cctgcaatcc taaataactc
     6361 cttatttact atagttacat ctatattgag tactgatact atagcttcag ctatattttg
     6421 aatatcagat tttatatatt ctaaacctat cataatcatc attccttatt tttctttagc
     6481 ataagtattt gtcttatttc tttaagaatc aagtatatct tatttaaagt aaattggatt
     6541 gacatcagga ttacaaagag cataacaatt attattaact cataatttat ttccatctaa
     6601 acatctcctt ataacaaaag actaggctta acctagtctt ttatattata tccatattta
     6661 tattttaact catttatctt ctttttccca cataaatata ttttaattta tttgactctt
     6721 taatcatacc atcaaagttt aatcttgaaa atatattaaa tacttcatgt ggatagtgaa
     6781 tctttgcaac agaaccacaa aatttatgtt taagatagtt taacttaact tttaaattct
     6841 ttaaatttaa atcctctaat ataacctctc cattgccctt taacactctt tgtatttcag
     6901 atgaagcttt ttcacaatct gtaataacat taaattggtc aactattatt atcttgttga
     6961 actgattatc atcaaatggt agaaactcta acttaccttt aactacattg caattttcat
     7021 tatacactag agaataacag acatcttcat aattactatt ctccagtata gttacagaag
     7081 ttccaagacc tctcaatctc ttgcctaggt ttcctatatg accaacaagc aatattttat
     7141 cacctacatt atactcaatt gtatccaaaa aacattctaa ttcatcagat gtatatttag
     7201 ctttaactgg ctgtttattt atctgacata atatgctttt cacaatgctt cacctcttca
     7261 tttcctcaat tataaattat gtacagatta cattataata aatggcgagc aactattcaa
     7321 gagatatttc attagtattt ttttaaacaa tctttataca ttttaatatg atattatacc
     7381 aaatttatta ggattttatt cataaaaatc agtctatttt ttatgtttat tcattgttat
     7441 tttcatccaa tttttttatt ttcgatatgg atacttcata tgccattttt ttgaccacat
     7501 tattttcatc tatttttttc tcatattctc tactttgaac cctaccccaa agctgtattc
     7561 tatcaccaac ctttagattc tttgcaaatt tagcatttct accccaaact attgatggta
     7621 tataatcaga tttattataa ggtctgttta tagcaactaa taaatcggtt atttcccttc
     7681 ctaaaggtgt cttcctatac acaggttcct tacatacata tccatctaaa aatatactat
     7741 tagggtcttt attttcctcg tctatacttt ttatttctct gacaaacact gtaagcacta
     7801 atctattttt attatttaat gttttgttat atgatcttaa ttggcctacg acattgacta
     7861 aattttgttt atcaaggtca atatcttgga gaagtctttc tgatacagtc attggaagta
     7921 tatcaaaaga atcgcttagt ctgtttattt ttattttcat attataaaat ttttctccaa
     7981 atatctcatg gctaaattct agcttattgt ctaactcccc tcttaagttt actacgttat
     8041 tctcatcttt aacagctatc atattttatc cctccacaat tgttaattct tatatttctg
     8101 gtatcagctt atataataaa tatatttata agggatgtat tttattacaa atctttgatt
     8161 gctgtttaaa agtttttatt tagttataaa tttcatttaa tatttcactt ttataattat
     8221 aaaattatta tctttttgta ccacatatac taattaaact acctatttca gttttgtatt
     8281 aattataaaa atcctattat ataattaata agtcatatat aacttaaatt tattcactct
     8341 atataagcaa cttcaacgaa ttaaatttat tgaaataatt cagatgaata ttgagtcatt
     8401 ataaacttat ttttcttaat tataagttat attagactta ttacaggtta tatttttatt
     8461 aatctgtatt aatatatgaa catttattga acttattgat tataaaattt aaatatttag
     8521 cttt
//
LOCUS       DAEOSP010000157         8476 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.160, whole genome shotgun sequence.
ACCESSION   DAEOSP010000157 DAEOSP010000000
VERSION     DAEOSP010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.160"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            249..1427
                     /locus_tag="KRH54_002804"
     CDS             249..1427
                     /locus_tag="KRH54_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG8580442.1"
                     /translation="MKYHIFLIITTLLIIELCNIYICKIASFGNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHHRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNKISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
     gene            1777..2658
                     /locus_tag="KRH54_002805"
     CDS             1777..2658
                     /locus_tag="KRH54_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG8580443.1"
                     /translation="MPAYLFDSNSITNIYRVFSILLSFIVTGLYFSYKNHKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            2867..3016
                     /locus_tag="KRH54_002806"
     CDS             2867..3016
                     /locus_tag="KRH54_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG8580444.1"
                     /translation="MTTVAEGIETSFQKDFLQEIDCDMLQGYVFSRPLPIKDFEKLMF
                     QNSSN"
     gene            complement(3276..5843)
                     /locus_tag="KRH54_002807"
     CDS             complement(3276..5843)
                     /locus_tag="KRH54_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBG8580445.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKA
                     LWGISEWVKQNNSINLYLQNSSAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQKISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     EPQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(5846..6451)
                     /locus_tag="KRH54_002808"
     CDS             complement(5846..6451)
                     /locus_tag="KRH54_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8580446.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPSDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(6825..8285)
                     /locus_tag="KRH54_002809"
     CDS             complement(6825..8285)
                     /locus_tag="KRH54_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBG8580447.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANVLVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
BASE COUNT         2820 a         1508 c         1187 g         2961 t
ORIGIN      
        1 ctatacttat acacataact attatactta ccatacataa ttactatatt tggacacata
       61 gttatcatac atactgtata ttaagatata atactttcta aattttatat aacacagaca
      121 catgcaataa aaaagaaggt atccataata agctctttgc atgtacctaa ataaaaactg
      181 taaaatagaa tatataaaat ctaatctatt ttaagtaaat acaatttcac tttaaaagag
      241 gatgtactat gaaataccat atatttttaa tcataactac tttattaata atcgaactat
      301 gtaacattta catatgcaaa atagcatctt ttggaaacaa aaatataact atacataaga
      361 attacaaata tattttttta ataaaaccat taaccttctt gataataata ttcacaaaat
      421 taccattacc actatattat atattgtttt acatagtaag tcataaattg ctcttaagca
      481 gaaaaagttc tactaacaac cattttattc ccaatatggc agtaataagt ttttgtattt
      541 tacaccttat tactgtaagt acaatatcac ttgttaaaaa tacaagtcca gatataatat
      601 ttgcaaatat gacctataag ttaattagta ttttaatagc ttctatttta gattttatct
      661 tatggtactc tatgaataaa ataaaagagc atatttatat aataactcaa aatcaaatag
      721 agttcagtct atttttaaaa tttatctggt tcactttagg atatatatta tttgatagta
      781 tacaaattac atttgctcca ccaagttctc taacttccta ctttatattg ggaagtaaca
      841 tattattaat cttacaacta attatattca tcaatcatat atattccatt gaaaaaaata
      901 tttatttaga aaaagaacat catagactaa aaatgataaa acaagaacaa ttactaaaag
      961 taaatttatt aaagaaaaaa gctaacattg atgaattaac atcagcatat aatcgaaaat
     1021 ttgcttttga aaaaatacag cattacattg ataacaattt attattttca ttgatatata
     1081 tcgatttgga taaattaaag acgataaatg atacatatgg acatttagct ggggataact
     1141 atattattaa cttctcaagt atagttattg aaaacttagg aatagatgac atatttgcta
     1201 gaataggagg agatgagttt atagttatta tgcctaaatc taatgaaaac accacaaaag
     1261 aatttataga gaaaataagt aaaattatgg ataataagat atcttttagc tatggtattg
     1321 tagaagtttc tagcgaaaac aaaatgactg tagaagaaat tattaatatt gctgatatta
     1381 gaatgtacac taataaacaa aataatcata aaaggaacaa taactaaaat gaatatttac
     1441 atgagcttac ttgtagtctc actatgtttt ttatcttatt atctctatca ttataatttt
     1501 tttaaatatc tagacataat gattaataac tctaaaaata aatacaaagt atatattgta
     1561 tactctcttt ttaattattt aatttttatt atttgtactt atttaaggtt acacctagtt
     1621 ataaattgga caatttttat gttaatttta tttatgcaaa ttaaagtaac ttacaaactt
     1681 tccacaataa aaaacctttt ttattcgtta aactgctcca tcattggttt agcaataaat
     1741 atatttttta ggtctttaat gtcaatagtg ttaaatatgc cagcatattt atttgatagt
     1801 aattctatta caaatattta cagagtattt tctatattgc ttagttttat agttacagga
     1861 ttatatttca gttataaaaa tcataaaaat aataagatta actttgaggc agttttagag
     1921 gacaaaaaaa acttaatttt cttattgagc cttatgtttg gaatgtactt gtatctatgt
     1981 ttgagtttgt caatatacta tactgttgga aatgtattga ttttaaaatt atatggaatc
     2041 aagtctgcca taatagtttt ggtaggtaac tatattgcag aaagatatag ctttagaatt
     2101 actcaaataa aaggttatga tattaaaaat caaatagaat atgtagaaag tgtaaagaca
     2161 gagtatgaaa ttaataaatt gaatgatatt gtatatacag atttactgac aggcttctac
     2221 aatagacctt ttgtaaatca aaaactatca gagtggacat caaatcacta caaatttacc
     2281 ttatgtttta ctgatttaaa tagcttaaaa cctgtaaatg ataaatttgg acaccaattt
     2341 ggtgataaat acatatccac tactgctcaa ataataaaaa aacttataaa tgtagaaaat
     2401 tccttactat ttagatatgg tggagatgaa tttttattcc ttttacagaa tacaagttgt
     2461 gtagaagcag aaaaaataat gattaatata aattatgaat tatcaaaatt aagtaatact
     2521 gacgagtatc cttatgctct ttccataagc tatggattag ttgagtggga tggaatatcc
     2581 gatattgaaa ctttaatatc aaaggcagat tctttgatgt atgaaaataa gcttaaaaat
     2641 aaatctactt taaactagct agttaagata ttctacaaaa tatatgttat aagagagtac
     2701 taagaatgtc aaaatatata gattttgcaa atattgaata cagtttacta atgaatgcac
     2761 tggatgtaag tgttagtaaa cacctattag atgaacactt tactgttgtt tgggctaatg
     2821 accgttatta tgaaatgttc ggctatacct agctaaaaag ttaaaaatga ccactgtagc
     2881 cgagggaata gagaccagtt ttcaaaaaga tttcttgcaa gaaatagact gtgatatgtt
     2941 acaaggatac gtgttctcta gacctttacc aataaaagac tttgaaaaac ttatgtttca
     3001 aaactcctcc aattaacaat ataaaattgc ttattataag tgatattatt gtatcaacaa
     3061 ataaaggaat tgtctcaaaa ttgatttttt ctaatcaaat ttttattttg agacattctc
     3121 atttttttgc ataagaaaat acccaccaag ctacggtttt tcttaccgct gttcggtggg
     3181 tattagtagt attatgaaat ctgaaaactt taagtattac tacttccgct aacgcaatca
     3241 caaaggtcga aagcttcaaa gcgaaaatag gctttttact catcaaacgt caccgtacct
     3301 gccaacccgt ccaccgttat catttgtccg ctcttaatag ttcgtgtggc aatgtgtgta
     3361 gccataacgg ctgggatgcc atattcacgt gcgacaatgc ttgaatggct aagaggacca
     3421 ccaatgtccg ttacaatcgc acttgcagat gaaaataatg gtgtccatgc tggagttgtg
     3481 gtaactgcaa ccaaaacact tccaggttga aagctttcaa aatctgctgg actattcagt
     3541 acacaagctg gagccgtcac aacaccagta ctggtaccaa tgcccgtcaa cacaattttc
     3601 ccatctttct gcttctgtgg ttcttgtgat atagtttttt tgtttttctc tggcaacttg
     3661 gatggtgaca cataacccat gtatttttta agttcagctt ttcgaacagg tattatcccg
     3721 ttcatatcag atagagactt gtttttatcc aactgctcta taagtacctc caattccgat
     3781 tttgtgagcc aataaatatc atctaaatgt gaaatagctc ctcctcgtat gaatcgttct
     3841 gagattttct gaaacataca acgaatcaga ggatgtccca ttcccatcaa ataaatagca
     3901 ttttcacgca taggagaggt ttcctgtgcc caatggagca acttaaaaaa tagtttttta
     3961 cgcaatccac ctatgtgttg taaaatctct tcttctgcat gttttctacg cttttcaaac
     4021 gtggtctggc gtaaaaaagg actttcccct tttccttcca caaatgtttt tacggattca
     4081 aaagttggtg taagtgtttc ctgcggtgtg gaatgcgcaa aatcaaactc ataagcggta
     4141 cgaccaaact ctttaaagta cttattgatt cgatttttcc attctatcca tacttcctgt
     4201 gaaactccaa caggcacaac tgaagacatg aaatcttctg ctatctttat tgcagaactg
     4261 ttttgcagat aaaggttgat gctgttattt tgctttaccc attctgaaat accccacaac
     4321 gccttttccg attgcagaac aatcgtatca aacccaagca ggaaaacgga agtatccgtt
     4381 ataccagcac gtcgaactgc tccttgaaat aattttgtaa ataatgtttc actgatagaa
     4441 gcagcaggca atgtaagctg aatccttgtg aaataaatac atgcggcata aaatattgtc
     4501 tgaataccct ccataatttg atgggcgttc atagtatgca ggggcttttc ttcccattgt
     4561 ttaatagcat cctcatatcc ttttcgtgcc gtttcccagc gtgcaacgct gttcataagt
     4621 gtcctacgta aagagcgtgg ggaaagggat ttgatagcaa tcaaaagtgg ctttgattta
     4681 gcagaaagat aaacatatcc attaataacc gtatatgccc cgttttttgg taataatttt
     4741 ttcgcacttt taccaaacat atctatccat aaaagctctg atgcacggtt tacgatttca
     4801 agaccaagcg tggcaaacaa aggtgtaaca ggatttggca agtgttctgc caaacttccc
     4861 tttgtgtata caacatcctg ttctggcaat acccattctg gtggcagaac agtaattgga
     4921 cgagcctgaa caatgtacaa tttatctttt tccagtgccc actctacatc cataggcatt
     4981 tgataatact tctcaatttt ctttccaagc tgtatcagtt gcattacctg attacgagta
     5041 agagcatatt tttttctcaa cctttcagga accatggttt cttctgttcc ttctgaagta
     5101 cgaaccgtca taatctcttt atttgccact tcatacgata taattcgttc ggaatcctta
     5161 tccactacga ttgtatcggg tgtaactaag ctagaaacca ccgcttcacc aagcccccat
     5221 gcagcgttaa taatcatttc gctacgtcta ccattgattg ggtttaacgt aaacataata
     5281 ccagatgaat cggaaaatgc aagcttttgt actacaacag caagtgcgac aatctcgtgc
     5341 tttatattat tcttcacacg ataagcaata gcacgagctg tccataagga agcccagcat
     5401 ctctttacag cgtcaataac tttatcttca ccttgtatgt taagataagt ttcctgctga
     5461 cctgcaaaag aagcgtcagg taaatcttca gcagtcgcag acgaacggac agccaccgat
     5521 atgttcccta atccggcata agccgtttta attgcatccg acacttcctg tggcatttct
     5581 ccattatgga aaagctcccc tattttctta gatacatctt caagttggct ggtattattg
     5641 gaatcaatac catctagcag tttattaatt cgagactgaa tatggttcgt ctcaacaaaa
     5701 atctggtatg aagcagtcgt aacatgaaaa ccgtctggca cagggatacc tgctgtcaat
     5761 agttttgata aagacatccc ttttccacca acaatttcaa tggttgcttg tttatgttcc
     5821 agtggcaaga cataataaat cataatcatt cacctccatc attcataatt ccgtgcagaa
     5881 atatattcat tgtttcatcc aacatttttt ccgaactatc cgatggtcgt gacgtgtagc
     5941 tgattgataa catggagtta attaacaaac gtgtcacaag agcggtatcc gttttgcgaa
     6001 agatgcctgt ggtcacacca ttatcaatta ccgattttat aatatcttga tatgcataac
     6061 gagcatcttt aagtctcttt cgatactctt cttctaaata atcagattct gtatttaacc
     6121 acatcagtac agttctatac tgctctggaa atctaagatg attaagcatg atttttctaa
     6181 ggcactgttg tggtgaaggt tcaccatcaa taatcctatg aacttgttgg atagtttttt
     6241 ctatttcttt ctcaactgca tatacaacaa tctcgtcttt cgttttgaaa aaatcgtata
     6301 gacttgactt tcccatgttg gctaaaactg caatttctct cattgatgtt ttttggaatc
     6361 cattttcagc catgagactc aaagcaacag caactatttt ttctcggcgt tctgcttgtt
     6421 gttcgggtgt aagcacaatt ccttttggca tagggcgata cctcctttta gcgaccactg
     6481 gtcgtttctt ttttatttat catacagcga ccaacggtca cttgtcaagg ttaatttttt
     6541 tcattgtata tggtactcga gataaaaatg ttcattttag aatttgctct atttaagtag
     6601 aaataggctt catcattaga aaacaagact ataaatttga gatatttatc atgttcaaat
     6661 ttcatcaata tcaaagttta agatattaat aaaaaaagaa ttactaagaa ataacatata
     6721 aagattcaaa aaaggagttg aacttaaaat ttaattacaa aaaatcaaat ttaagacaac
     6781 tcctttattc tttatctcta ctattcttta tctctattta tttattacaa ctccctaaca
     6841 tttaccacta ctgccttagg tctagtcata gcctcgattg aatacttaac accttgagtt
     6901 cccataccag atgcctttac acctaaaaat gggaagtggt ctggacctct ttcagtctta
     6961 ttatttattt gaacagtacc aacttcaagt ttatcagcta tataaaatgc cttatcaata
     7021 tcacttgtaa atactgatga ttgaagccca tactctgatt gattagcaat ttcaattgct
     7081 tgatttatat ctttaactct tataattgga agtactggac caaatggttc ttcccaagca
     7141 attttcatat caacatttac attatctaac aatgttggat agaataaatt attttttcta
     7201 acattgcctg ttattaactt tgctccttta tgtaatgcat catcaaccaa cccttgaaca
     7261 aaatcagttg ctttattatc tataagagga gtgataacta cttcatccat tggatttcca
     7321 attgtcaact tctctaccaa tggctttatt tttccgacca atttatccgc cacactctct
     7381 tgtactaaaa ttctctttac tgccgtacat ctttgtcctg aatatgaata cgctccagat
     7441 actatattct tagctgcaaa atctaagtct gcatcttcta atactatagc cgcatctttt
     7501 ccaccaagct ccaataataa tggtaccatt ccagatattt tagatatatg ctgaccaact
     7561 tcagtactac cagtaaagtt aataaaatta attcctttat gcgttacaac ataatctcct
     7621 atctcactac ccctacctgt aatagtatta agtactccat ctggtatccc agcttttctc
     7681 ataatttcta ccacatgaag tgcgctaata gccccttgtg tagcaggttt taatacaact
     7741 gcatttccac caataagtgc tggagcaatc tttgacattg ataaatttac aggataatta
     7801 aaaggagata tagctaaaac agtacctaat ggaacccttg aaacataaga catcttattt
     7861 cttgttccac cagggaaatt atctccacta atggcttcac cctccatatt ttttcccaca
     7921 tcagctgtgt atcttaaaaa atctgctgtt ctctcaactt ctgcccttgc agattttatg
     7981 tctttggcta tttcaagtac cagtacatta gctatctcat ctatattttc taacaataaa
     8041 tcagcagcct tatgaaatat attggctctc ttgtatacag gcatttcagc ccatactttt
     8101 atactctctt ttgtgttttt tataacctca tctacctcat gttttgtcat agattgtact
     8161 ttcccaacaa gagaattatc tattggtgag taaatttcta ttggctttct cgaattagat
     8221 tctacccatt ttccatttat taaattttta tatacatttt cttttgtttt aagttcatta
     8281 aacatatttt tattcctctc tctgatataa ctttgtgtat ttaaattata gccatttatt
     8341 cgttatgtaa acatttcttt ttttcataaa cgtttaccta taaaaaaaat tttaagttaa
     8401 cgtttttcta cattcttcta ttttaataac atactcgctc caaatctata taggtcacac
     8461 tttaatatca atatac
//
LOCUS       DAEOSP010000158         8443 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.161, whole genome shotgun sequence.
ACCESSION   DAEOSP010000158 DAEOSP010000000
VERSION     DAEOSP010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8443
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.161"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(252..1121)
                     /locus_tag="KRH54_002810"
     CDS             complement(252..1121)
                     /locus_tag="KRH54_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG8580448.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(1218..2075)
                     /locus_tag="KRH54_002811"
     CDS             complement(1218..2075)
                     /locus_tag="KRH54_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8580449.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            complement(2289..4307)
                     /locus_tag="KRH54_002812"
     CDS             complement(2289..4307)
                     /locus_tag="KRH54_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBG8580450.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVISQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            complement(4578..5354)
                     /locus_tag="KRH54_002813"
     CDS             complement(4578..5354)
                     /locus_tag="KRH54_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBG8580451.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(5411..6382)
                     /gene="asrC"
                     /locus_tag="KRH54_002814"
     CDS             complement(5411..6382)
                     /gene="asrC"
                     /locus_tag="KRH54_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBG8580452.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(6395..7186)
                     /gene="asrB"
                     /locus_tag="KRH54_002815"
     CDS             complement(6395..7186)
                     /gene="asrB"
                     /locus_tag="KRH54_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBG8580453.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(7187..8194)
                     /gene="asrA"
                     /locus_tag="KRH54_002816"
     CDS             complement(7187..8194)
                     /gene="asrA"
                     /locus_tag="KRH54_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBG8580454.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
BASE COUNT         2728 a         1519 c          757 g         3439 t
ORIGIN      
        1 gtaaagttac ttaataaaaa gttactttac tttttattat ttaaattcct gtttttatta
       61 taataggtaa aattctatta aactagttca cattttcatt attattgata tattatatat
      121 gaatatacca ttttgttttt taataaaaaa atcatgctac atcaatagta agcaactatt
      181 aatgtagcat gattttttgt atctaaataa tcttagtatt tacctatcaa tatagattga
      241 tatatcaaaa actatagtct tctatattct tctcttttta gcttttggtt acttagatag
      301 taatcaattt catcaattag acgttgttta tcatcaggta atacaccttt taaattagca
      361 taatttgatg catgattact tctaaataca attttttcat tagcatctat attctttatc
      421 atctctttaa tttcttgtaa cacagcctta tcctctagta ttgtaaactc tcctgcctta
      481 agtaaatcgt aaagttcagt attaggttct accattaaac taagtacacc cacataatct
      541 ggtgaaattg ctcctaacat ttttcctgta tcaactgcat gctgatgtgt ttttttagtt
      601 cctccaagac ctgctattac agtggctgat aatttaatcc cacttttttt aacttttcta
      661 ccaactttta ttaattgttc actactaaaa cctttcttaa tatcttctaa tacttcattg
      721 cttccacttt caagccctag ataaatcatt ttaacaccaa gttttcttaa ttctttaagt
      781 tcatcatcag attttttctc tatagattta ggagagccgt acatagtaat tctttcacac
      841 tctggaaaaa cttcttttat atataaaata atatctctta atttctccat tggtattatc
      901 aatgcatctc catctgctaa gaatattctt tctacatgct tataatatct tctaaatata
      961 tcaatttcat tttttattac ttctagtggc tttattttaa attttttaga tttgtacata
     1021 ctacaaaacg tacatttgtt atgagaacat cctagtgtga cttgtattat aagactataa
     1081 gcctcacttg gtggtctgta aagtggcata tcgtattcca tattaaaatc ccccttgtaa
     1141 taaaatattc attctattat attattaata tttactttaa ataataactt ataaaattat
     1201 attttttaaa ttttagttta agatatattc tttaaaaatt taatgatact attggcagta
     1261 atattaagtt gtttttcttg acttataata gcatcattat ctcccttttg ttttccataa
     1321 tctccaaatt gtgcatgatt tcctccttca atctcaacat aagttgtatc atcaggaagt
     1381 ttttgttttg attctacaag acttttaaaa ttgactactc catctttgct cccccatata
     1441 gatataactt tttttcctaa ttctttcaac tcatcaccca ttggataaga agctaaaaat
     1501 acaacgcctt caattaattt attatcacgt gcaaaatttg atgctgcaac tcctcctaat
     1561 gaatgacctc ctattaccca atgttctatg ttgttgtaag aatccataac tttctgtgcc
     1621 ttattagttc caagtatagc tagattaagt ggcatcttta cgattacaac ctcatatcca
     1681 ctttctgcta tttttcttgc aagtggagca taagattcta tttctacttt agctcctgga
     1741 tacaaaatta aacctttaga aacttttctt ccttttggtg taaatgaaat gttttcattt
     1801 acagttactt caactttact attgctgact agagcttctt ttgccagctt ttgaggttca
     1861 tatgttttat ttatccatat gaaaaagcca agcaaaatca atacaaatag tatacctatt
     1921 cccttaaata tcttattctt tatgttaaat tttctattat tatttttatc atctttgttc
     1981 tcaacctcat cataaagtaa aggtctctca agttccatag ttatactaaa tgtatcatat
     2041 ttcttattac gctttacctt tacatcttta ctcataaatc taacccccat aatatttgaa
     2101 tactattata gtttatccta agtaaattgt tttttcaacc aatgaaaaaa atcaatattt
     2161 taactatttt catataaaat tcataaatat atgtttttgt tttttatata ttattttctt
     2221 ttagtatttt ataaaacaaa tagccatata ctttatttta aatatatggc tattctataa
     2281 tttaatatct aagctgcttc ttttattgaa actttagtta tattacttat tctatacatt
     2341 tctttgacac catttagagt ttgcagtaag tttagacata ttatcttgtc acctagactt
     2401 ttagtattta caatactctc taaaattaca cttcttcctt catcatactt atctacattg
     2461 taatttgaaa tataattggt atctctcaat attttttcaa catcttcatc ttttatttta
     2521 tttttattaa catttatata tagattatac atattactta ttagtaattt ctgattttct
     2581 aaaaaatcct cttgatgttt atctttatat atagcattta tcaacataag tctatcttca
     2641 ataagtgatg gtatcaataa taaatttttt atatagtaaa catcagctac attttctata
     2701 gacttattga cttcatcaat tatattttta actattcctt tatacatttc tataacatgt
     2761 tgatagcctg ttttagcatc atatggtaat ataaacttat ttactattaa tgcaataatt
     2821 gctcctaatg ctacatatga aatcctgctt attgttaaaa catttgggtc tcccataact
     2881 actgctgaac caagagctga aacagttaca cataccatta attttctata atcaactaca
     2941 taagagttta tataccctgc taaaagaact attaacgacc ttaatgtact atcttttatt
     3001 attgaaaata atacaataaa tattacagct cctataaatg taccttctat tctttttcta
     3061 gacctttgta tacaattttc tgcgtaaggt tgtgttaatg aaaagacagt taacattatc
     3121 catcttccat ctcttaaatg gaaataatcc ataataaaac ccgctacagc tgtagctaaa
     3181 gcaatcttta ctgcataaga aaatcttata gaatttaaat taaagttttt cttatatatt
     3241 gatagtcgtt taaattcatg aggtatcttt gattttcttt cagctttctt ttcgtctctt
     3301 gattttactt ttttataatc tactaaaaac tcatataaat tttctaaaac actataaatt
     3361 tcttttaaat ttatattttt agtatcctga gaaataacat aatctctaaa tcctaatatc
     3421 tctttatcag ttatggtatc tttattttct aaacacatct taatatagtt tatttctttc
     3481 gttgctacaa ctaaaacatc tgatttacta tttatgctac tttcttctaa aatatttttt
     3541 atatcttcat cttcaaattc tttactatcc tttttgtatc tttccaggat taaacttatt
     3601 ctttctaatg aaaataaaat attcattatg tctatacctc taatactgat ataaaagtca
     3661 tcttttctat tatcaaaaat aaccattttc aaatttttag atatagaatt tattctataa
     3721 ttacagctat ctatttttct catactagat ttaattatag aactatcatc attaatagta
     3781 ttttttaaaa gactaatttt ttctaataat tcatcacata tagatatcag ttctttatct
     3841 ccgacatttt tcatcctatt tctattcata acaaattgag caatcattat tataactgct
     3901 ccaacagcta aagccgacaa tctcatagga aattcacttt ttcctacagg tatacttacc
     3961 ataaatatat acattagacc aaagggtaca tatactgctc tttttaagtc ataactaaat
     4021 acatacccta ttataaataa tgctataaaa tttaataaaa ctcctaaata catattttgt
     4081 actgcaaaaa atgatagtat tcctaagatt atatttattg ctaaatactt cagtaaattt
     4141 ttaattggag aaatagttaa atctctttcc aagagtgtta gcatagctgt tactatacct
     4201 acacctataa gtgtattttt acttccaaat aaatatataa atatagaaat aaatgctatt
     4261 ataaaaataa acaactttgt ttttgatatt actagtttct ttgtcatcat atccccccta
     4321 aaaatttcaa caagaccaat tttatcatat ttgttatctt attcaaaatg cgctttttta
     4381 cggtaggact ttaacttttt ttatatttag aactatataa ttcatatata aacgtatttt
     4441 ttatatatat aaccgatata ttttcgtatg ctcccatatt ttttttgttt caaatatttt
     4501 ttcaaaaata taaaaataac tctctcaaca atactaaaaa tgtatttatc aagagagtta
     4561 taatttatac aataaattta attaagcttt tctttaccta atatataagt tccaagaccc
     4621 ataaataaag ctcctccaac aatatttcca agtgttacag ctactaaatt ataaatagct
     4681 cctccaatag taactgtact tattgttggt gaaaataatg aaacactata aattgtcata
     4741 tttgctacac tatgctcaaa acctgatgtt ataaaagcaa ataaacataa gaatatcata
     4801 attatttttg cagtatcact attagttcta aacgaacaca acactgatac acatactaga
     4861 atattacata aaatccctct aaagaaaaga gctgtaaatg gcatggatgc tttactagct
     4921 gctgtatttg caaaaaattc agctacaggt cctttgtcta ctagaccagt tcctacaaat
     4981 ataatgccta aaaccaaagc tccgattaaa ttacctaccc aactgtatgc ccatatttta
     5041 gaagtatctt ttatacttac acctttattt aacataccag cagacataac catattgttt
     5101 ccagtaaata actctgttcc cgtcattata accaaactta gtgctattgc aaatgataac
     5161 cccattacta tcttagtcat aggtgaacct gcatcagtca aaagtcctcc aatagtaaat
     5221 atcaaaagta tacctatacc tacataaagt cctgcaaacg cagaagatac caaatatttt
     5281 actttacttg tgtttaataa atttatctta tttatagctg cattagttaa tttatccaaa
     5341 gtttctttat gcataactcc ctcttccttc tattaaattt taatttattc aaatatatgt
     5401 tttactcaat ttatagatat ttaattccac tccagtatat gttttcgtac attttagtta
     5461 tttctggaag ctctacacct tcaagtgccc attttttaaa ttccataaat cctgttctat
     5521 caattatata tcctatatgt tcttttcctc ctggtgcatc tttatctata tacttttcta
     5581 catatctata agtattaagt attatcttta ttatgctgtc ctcatctacc cataaaagaa
     5641 aatcttctgc tagtctaggg tttttctttc ctgttctacc cattatagct aatctatagt
     5701 attttttctc atctctagtc catgcatttg ttgggcaatt tagtacacac tctccacagc
     5761 ctatacactt atctttatct cttaccacct tattattttc tgtttttaat gctccaacag
     5821 atatcttaga acattttttt acacatgctc cacaatttac acatctatcc ttttcatata
     5881 ttggtaaagc cataccaata attccaaagt cattcattct agcttttata caatcatttg
     5941 gacaaccagt taaagccact ttaaaatgta aatcatttgg aaatatagct ttttctattc
     6001 ttttagcaaa ttcagttgta ttatattgag cctttggaca gactttattt cctatacaag
     6061 ctgctatatt tcttgtacct gctgctggat aacctgcgtt tttctcactt tgatttatat
     6121 tcattttttc aataacaggt tgtataattt tatttatttc ttccatatct tccatattta
     6181 ttcctaatat ttcaaacccc tgtcttgtag ttatgtgcac ctgtccattg ccgtactcac
     6241 ttgcaatttt agaaactaca gctaaactct cagggtcaat aagacctcca ggtactctaa
     6301 ctcttaaaga agtctcatat tttgtttttg taattctata agcatttttc attacttttt
     6361 tggtatttaa atctcttatc acaaacttca ccccctaatc aataagattt ttagcatatg
     6421 aataatcaaa tactggtcca tctaaacata tgtaagtatc atccatttta caatgtccac
     6481 atttacctat tccacaacac atttttcttt cataagaaac ccaaatgttc ttttcatcta
     6541 aatctctttt taaaaattct tctactgtaa atttcatcat cattggaggt ccaactacaa
     6601 ctatagaagt attgtttata tctttcatat ccaattctgg tatgtatttt gttactaatc
     6661 ctacattacc actatatcct tcctctgctc catctacagt aactaaaata tcaagttttt
     6721 tactccatct ctttaaatca tctgcaaata aaatatcttt tggtgattta aatcctacta
     6781 ttaatttaaa cttttcgcat ttttctttat tattataaaa atattcaact attcctctta
     6841 caggggctaa tgcacttcct cctgccacta ctacaatttc tcttccttca taaagggata
     6901 catcaaatcc atttccataa ggacctctta taaaaaaaga atctccttct ttataattaa
     6961 acaactcgct agttactttt cctacatttc ttattgtaaa gtcaatataa tcctctccat
     7021 atcctgatac tgatattgga ctttcaccgt atttaggtat agatatctca taaaactttc
     7081 caggcaatac accttttgta ttgcagttta ctctaaaagt ccattcttta tcagtatgtt
     7141 taactatatc tagtattttt gcacttactg gtatatatgg gttcatttat acttcctcct
     7201 tactaattac taaatcattt attttttcta tacactttga aaatgatatg tattgcggac
     7261 aggcatcatc acatcttcca caaccaacac acatatgata tccgaatctt tttttaaaat
     7321 catgtatttt atgcatagtt ttaaatctca ttctttcacc ttgttttttt ctaaaagaat
     7381 ttccacctgc tatatctgta tatccatcta cttgacaaga agcccaaacc cttcttctct
     7441 cacctacatt ttcgttttcc ttatagaaaa tatcctgcat tgtaaaacat gaacatgttg
     7501 gacatacaaa attacatcta ccacatccta tacatcttgt atcatattca tcccatattg
     7561 aatttcctat catttcattt gaatcaatat tctctggcac atctacagat attaaattat
     7621 cagttacata atcaacatta aattcagttg cttctccatt aaatacttca aattcactat
     7681 cctttatatc taagtacagt gtatctcctt ctatatttaa tccaattgaa taattatcag
     7741 ttttgttaga atccatgctc acacaaaaac agtttctaaa actttcctta caccctacaa
     7801 gtgcaaactt tactttatct ctgatatttt tataaaaata atctttttct tcaccattat
     7861 ttagatatat ttcatcaagt ctttttatac catttatgtc acaagctctt aagaaaatta
     7921 aaattttctt gttgtctaaa tcgctttcct taaactcttt ttcagtaaaa taaaacaata
     7981 cttgatttat gggaagtaca acttcttttg gtgaaaagtt tgctttctta gcaaattcca
     8041 tttcttcaaa agcgtttacc tccttgtatc ttataacatc agtatctgaa aaagtacctt
     8101 taaatgggat taaaactggt gcaaatatct tgtagtcttt ttttagataa ttcagacctt
     8161 cattgaactt agatgaatct aattttagtt tcatttttat cacctttctt atattttagt
     8221 tttacttact tgctatcttg tttaaatctt aacactttat tttctatgct tccgtgataa
     8281 ttatcacagt ttcaaaaata ataaaaaaaa tattaatgga gtttattaag ttctgttaat
     8341 atttttttat tatctacatt attcaatttc aaatctaatc acttaaatac aatcattaag
     8401 tacaataaaa ctactatcta ctcaattttt tgaataaaaa aga
//
LOCUS       DAEOSP010000159         8441 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.162, whole genome shotgun sequence.
ACCESSION   DAEOSP010000159 DAEOSP010000000
VERSION     DAEOSP010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.162"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            23..2146
                     /locus_tag="KRH54_002817"
     CDS             23..2146
                     /locus_tag="KRH54_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBG8580455.1"
                     /translation="MDSYRQLKAKIPCKETGIEIKRTLCDICSPGVHCGIDAYVKDGK
                     VIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKRIGKRGEGKFKPISWDEAY
                     REIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSFGSPNYGTESSSCSTSAQM
                     AGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLIKLKEKGLKFIIVDPRITP
                     TTQKLADLHLRIKPGTDGALALGMANLFIQKNLIDREYIEKYVHGFNEYKEYVKLFDL
                     DTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQNGFQNYRAMMSLSAITGNY
                     DRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERIGGERFPLWNEMVTQFQAM
                     DLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSVDFFVNIDLFMTDTSKYAD
                     ILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVEILSDLANAMNIDDDLLKA
                     GYEASIRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSYTREGYDTSTSRFELKSTI
                     IEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNALHSRLHDIKLSRILKNEAS
                     CDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLYKNIHMYHGYREADINSII
                     CDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            2160..2582
                     /locus_tag="KRH54_002818"
     CDS             2160..2582
                     /locus_tag="KRH54_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG8580456.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            2990..3793
                     /locus_tag="KRH54_002819"
     CDS             2990..3793
                     /locus_tag="KRH54_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG8580457.1"
                     /translation="MKKLKLIIMFALVSALLVTGCSSGNNKNKEEQKVIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMIEPPIQFGAC
                     SFALQKNEKGEKWTKEINEVIEEMYKDGTLTKISENWLGKDITKEPINE"
     gene            3786..4454
                     /locus_tag="KRH54_002820"
     CDS             3786..4454
                     /locus_tag="KRH54_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG8580458.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVSFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMAEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     RRAY"
     gene            4690..5352
                     /locus_tag="KRH54_002821"
     CDS             4690..5352
                     /locus_tag="KRH54_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG8580459.1"
                     /translation="MAFNMKYFLELFPTIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMSDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDTGQIEAAYAMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            5412..6209
                     /locus_tag="KRH54_002822"
     CDS             5412..6209
                     /locus_tag="KRH54_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG8580460.1"
                     /translation="MIKRNIIKCLVAVLSLTFVITGCSKKEEVKNEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVATSVNKD
                     NLFQEKVYGDGTSGLQDLDLGRVSAWIDAELTIVGDAKKANMKVRVLDKKLSATNIAY
                     FFKKNDDKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK"
     gene            6327..7517
                     /locus_tag="KRH54_002823"
     CDS             6327..7517
                     /locus_tag="KRH54_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8580461.1"
                     /translation="MEVLQMIETKIKSIALKNREKIIKIRRQIHSNPELAFKEYKTSK
                     LIKEELNKLNIEYINIAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSIN
                     DNMHACGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGV
                     DALATGHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAV
                     YSSIQQIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKI
                     LEYIVKNVCKAHGADCKFEKSTGGDAVINDKNMIEIGRESACKILGAENVEIIDFPAM
                     TGEDFAIYMKEKPGLFMYIGVGDKEKNINYRLHSNKFNIDEKCLSIAPSLFSQLTVDY
                     LCQL"
     gene            7626..8375
                     /locus_tag="KRH54_002824"
     CDS             7626..8375
                     /locus_tag="KRH54_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG8580462.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEISQSEVVAILGSSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDITNLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSKKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIIRKEEVFS"
BASE COUNT         3341 a          854 c         1572 g         2674 t
ORIGIN      
        1 attgggaaag gaaaatgtta atatggatag ttatagacaa cttaaagcta aaataccctg
       61 taaggaaaca ggtatagaaa taaagaggac tttatgtgat atttgttctc caggagtaca
      121 ttgtggaatt gatgcatatg ttaaagatgg taaagtaata aaaatagaag gtacaaaaga
      181 acatccaatg aataaagggt tgttatgtac caaaggtctt tgcaatagag aatatatcta
      241 tagagaagat agaataaaaa cacctctaaa aagaattgga aaaagaggtg aaggaaaatt
      301 taaaccaata tcatgggatg aagcttatag agaaatagca gaaaaattaa atagagtaaa
      361 atctgagtat ggagcaaagc atgtagcttt ttttacgggt tatggaaagt ggtatagacc
      421 ttttttacaa aggtttgcat cttcatttgg ttcacctaat tatggtacag aatctagttc
      481 atgttcaaca tcagcacaga tggctggaaa tgttgctact ggaagaaact ttacaatgca
      541 agatatggag aatgcaaaaa cttatttagg gtggggattt aatccatact actctaaatt
      601 tttaaatagt gtgaatctaa taaaattaaa agaaaaaggt ctaaaattta taattgtaga
      661 ccctagaata acaccgacaa ctcaaaaact agcagattta catttaagaa ttaaacctgg
      721 aactgatgga gctttagctc ttggaatggc gaatctattt atacaaaaga atttaataga
      781 tagagaatac atagaaaagt atgtacatgg atttaatgaa tataaagaat atgtaaaatt
      841 atttgattta gatactgtaa gtagaattac aggagtttct aaagataata tagtaaaagc
      901 tgttgagatg ttgtcacaag gtccattttg tataaatgaa agtgcagctc caatagttca
      961 ccatcaaaat ggatttcaaa attatagagc aatgatgtct ttatctgcta taacaggtaa
     1021 ttatgataga ataggtggaa acataccaaa acaagattac tttgctggta agataggtgg
     1081 attccaaaca ctagaaaaag agtttgtaaa agaagttcta cctaaaaata taaatgaaag
     1141 aattggtgga gaaagatttc ctctatggaa tgaaatggtg actcaatttc aagctatgga
     1201 tttacctagg caaatagaag aaaagacacc atatgaaata agagctttgt tttgtcatgg
     1261 gataaatgct agaatgtttc ctaattcagg gagacttttt aaagcgttga atagtgtaga
     1321 tttttttgtg aatatagatt tatttatgac agatacatca aaatatgcag atatactttt
     1381 acctgcatgt acatcatttg agagagaaga atgtaagagt tatccaggag ggtatattac
     1441 atataccaaa aaagttattg ataaattata cgattcaaaa tcagatgtag aaatactttc
     1501 agatttagca aatgctatga atatagatga tgatttatta aaagcaggtt atgaagcaag
     1561 tataagacat atgtttaaaa atacatgtgt agatgtagat aaacttaaag aaagtgattt
     1621 accattaaaa tgtaaagatt ttaagccata tatagtaggt tcatatacaa gagaagggta
     1681 tgatactagc acttctaggt ttgaattaaa atctacgatt attgaaaaat ataaagattt
     1741 tggactggat gcactaccaa catatagagg atttaatgat aatatagatg atgaatatac
     1801 tcatttattg acatcaggag caagaatttc aaatgcacta cattcaagac ttcatgacat
     1861 caaactttca agaatattaa aaaatgaagc tagttgtgat atttctagct tagatgcaaa
     1921 agaattgggt gttatcagtg gagattatat aaaaataact actaagatag gaagtttgga
     1981 tgtaaaagtc aatgttactg atagagtctt atataagaat atacatatgt atcatggata
     2041 tagagaagct gatataaata gtattatttg tgataatttt gacccttata gtggattccc
     2101 tgcgtataga agttgtaagt gtaagattga aaaaatgaat agataaatgg agagatataa
     2161 tgaaaagttt atttaaattt aataaagaat tatgtacagc atgtggagca tgtttgcttg
     2221 catgtataga ccaaaatgat aataatatag acaatggtga actttttaga catgtattta
     2281 caatagagga aggtgaaaat atagaatatt tttcattagc atgtatacat tgtaatgagc
     2341 caaaatgttt aggtgtatgt cctaaaaatt gttttaaaaa agaagaagga ttcgttgtat
     2401 tagacaatca aaactgtata gggtgtaaac tttgtgaaaa agcttgtgaa tatggagcct
     2461 taatttatgg aaatgatggt aaggcaaata agtgtaatgg ttgtattgag agatataagt
     2521 gtggtatgga ttcaccttgt gtaatggtat gtcctacagg agcattaaaa tttaataaat
     2581 aaattaaata tagagaatga cttatactta ggtataagat atttttacaa aaagctgtat
     2641 tgagtttgtt ataatgaaaa ctcaatatag cttttttatg gatatagttg gatactataa
     2701 tgtgattctt agtaatagtt ttttgctatt ggacaaagac tgtaaaacat attaaactta
     2761 tgttgttcaa agtcgaagta taatataata actagaaaat tggtttatag tgctataaaa
     2821 gcaaatttag taaggtataa aatcagaggc aatcctttac tataatatca atatttggga
     2881 gttctgttat tgcagagcta ttaaacaatt ataatggtac aagtgattca acctttgaaa
     2941 atgtattata taaaaaatta aaataagatt aaaaaggaga gaataaatta tgaaaaaact
     3001 taaattaatt attatgtttg ccttggtatc agcactgtta gttacaggat gttcatcagg
     3061 aaataataaa aataaagaag aacaaaaagt tataagagtt gcaacatcag gaacatatta
     3121 cccttttgct tttaaagata aagataaact taaagggttt gaagtagatt tttgggatga
     3181 atttgctaaa cgaactaatt gtaaagttga atgggtatta actgatttta gtggattatt
     3241 tggattatta gaagctgata aagcagatgt tgtatctgct caacttactg caacacctga
     3301 aagagagaaa aaatatgctt tttcagatgc ttataattat agtggaacaa acattattgt
     3361 tagagaagat gatactagta taaaatcaat agatgattta gttggcaaga aagtaggtgt
     3421 gggtactgga gcagtagcaa atgaaatatt aaaagaaaaa tacccaaatg atgaaataaa
     3481 aatagtaaat tattcaagtg cgactcttaa agggaattat caagacctag aaatgggaag
     3541 attagatgca gtagttgcgc aggatgttga ggctttaata gctataaaag aacaaaaatt
     3601 aaatcttaaa atgatagaac ctccaattca atttggtgcc tgttcttttg cgcttcaaaa
     3661 aaatgaaaaa ggtgaaaaat ggacaaaaga aattaacgaa gtaattgaag agatgtataa
     3721 agatgggact ttgactaaaa tatctgaaaa ctggctagga aaagacatta ctaaggagcc
     3781 tattaatgag taattttaat tttgaatttt gtctaagtat atttccattt ttgattaaag
     3841 cattgggaat aacaatacta atatctattg tttcattttt tttatcaata ttattaggtg
     3901 taatacttgc ctttttcaca cagacaaaat caaaagtttt acaatacata acaagagtgt
     3961 atgtttcatt ctttagagga acaccacttt taattcaaat atttttcttt tattttggat
     4021 ttccaatgat attttctttt atgaaagatt gttcacctta tttagcaata gtaatttgtt
     4081 taggtgtaaa ttcatctgca tatatggctg aaattatacg agcatctatt gagagtgttg
     4141 ataaaggaca acaggaagca tgttatgctt ttgggttaag taaatatcag tcaatggtta
     4201 aaataattct acctcaagca gctacatctg cagtacctcc tattgtaagc tgtacactgg
     4261 acataattaa aggttcttct ttaggtttaa ctataggaat acaagattta atgggagtag
     4321 ctcaagtaga ggctgcttca agttttaaat attttgagat atatatgata acgatattaa
     4381 tttactgggc agtatctata atattgagta atatacaaag caaagttgaa aaaagaataa
     4441 gaagagcata ttagatttca atatttaaaa atgggatgat atagatatat tattacttgt
     4501 aaataaattt ttaattttag aaaaataaac ttagtaatct aaattattga gaaaatatag
     4561 tatcaaaatg gactgtataa tcaagttaaa aaatagtttt tctattgaaa aaataaataa
     4621 aattctattg taatttaata tgcgataaag ttttataaaa aacaacatta agctaggggg
     4681 acttatttta tggcttttaa tatgaaatat tttttagaat tatttccaac aataatgaaa
     4741 tcgttcaatg taactgtgat tttggcaata agttcattag tattttcatt aattataggt
     4801 acaatagtag cgttatcatc ttattacaaa gtgaaagtat tgaatcaaat atgtaaagta
     4861 tatgtatcta tttttagagg tacacctctt atgccacagt tattcttttt atattttgga
     4921 ctagcatata tgagtgattt tgttaaaaat atggacccag tatttgcaac atctatagta
     4981 cttagtttaa atatgggagc ttatatgtct gaaacgataa gggcatctat actttcaatt
     5041 gatacaggtc agatagaagc agcatatgca atgggattaa caaatttaca aacaatgaaa
     5101 aggatagtga taccacaggc cgtaagagtt gcactgccat cattattcaa taattttata
     5161 gatttaatta aagggtcttc tgtagctttt gttgttggag taaatgatat aatgggtgct
     5221 gccaaatcac aaggagcgtt gagttttaag ttttttgaag tgtatggagc agttatggta
     5281 atttactggt gtataataac tcctttaggt tgggggcaaa aaaaattaga aaagaaaata
     5341 agcaattatt aagttattaa gaaaatacat aaaagtattt ttatataaaa aatggtgtga
     5401 ggagaagaga tatgataaaa agaaatatta taaaatgttt agtggcagtt ttatcgttaa
     5461 cgtttgtaat tactggttgt tcaaaaaaag aggaagtaaa gaatgaaaaa gttataactg
     5521 tagcttcatc taattcagct gacccatatt cttatgttga taatggtgag cataagggtt
     5581 ttgaagttga catgtggaaa gagattggta aaagaagtgg atataagata aaaatggaac
     5641 caacaggttt tagttctata tttggtatgt tagattctgg taaagttgat gtggcagcca
     5701 acttctttgg aatgtctaaa gaaagattag aaaaatacga tgcttctata ccttatggtt
     5761 ctgattctgt gtgtatagct gtaaaagatg gaaataacga aattaataag cttgaagatt
     5821 taaagggaaa agttgtagca gtttcagaag gttctcaagg tcaacaggtt gcaactagtg
     5881 ttaataaaga taatttgttt caagaaaagg tttacggtga tggaactagt ggtcttcaag
     5941 atttagattt gggaagagtt agtgcatgga ttgatgcaga gcttactatt gtgggagacg
     6001 ctaaaaaagc taatatgaaa gtaagagtat tagataaaaa attatcagct actaatatag
     6061 catacttctt taagaaaaac gatgataaat ctaaaacgat aaaagaagat gtaaacaaag
     6121 caatagaaga aatgcttgca gatggaacag tgaaaaaaat atctgaaaaa tggtttggaa
     6181 tggatgtaag tgctgatata caaaaataaa tactttaaaa ttaaaatgta aaactgtgat
     6241 aactatagat aagtatacta aatttatcta tagttatctg tgtattacaa tgtatttaga
     6301 ttagtcttta taaatcaaaa tgagttatgg aggtattgca aatgattgaa actaaaataa
     6361 aatccattgc actaaaaaat agagaaaaaa ttataaaaat aagacgacag atacacagta
     6421 atccagagtt agcatttaaa gaatataaaa catctaaact tataaaagaa gagctaaata
     6481 aacttaatat agaatacata aatattgctg gtacaggagt attagctact ataaaaggaa
     6541 aaaataatgg tggaaaaact atattactca gagcggatat ggatgctctt ccaatcaaag
     6601 aggaaaatga tttagaattt aaatcaataa atgataatat gcatgcttgt gggcatgatg
     6661 cacatgtttc atggttgtta ggaacagcaa tgattttaaa tgacataaaa gaagaattga
     6721 atggaaatgt aaaattacta tttcaacctg gcgaagaaaa aggtggttct gatattgtaa
     6781 ttaaagaaaa tgtacttgaa ggtgtggatg cattagcgac tggtcattgt tggccaacta
     6841 ttgagtcagg aaaaattgga atcgctagaa attgtgctat ggcagcgact aatacttttg
     6901 aaataacaat aattggcaag ggtgggcatg gagcagagcc tcataattgt atagacccaa
     6961 ttgcagttgg aaatgcagta tattcatcca ttcaacagat agtttcaaga aaaattgacc
     7021 cagttgtacc tgtagtagtg tctgtttgtt cttttaattc tggtgtttct aaaaatataa
     7081 taccagatgt atgtacactt caagggacta ttagagcgat aagcaaggaa caagtaattg
     7141 aagtaagtaa aattctagaa tatatagtaa aaaatgtatg taaagcacat ggagcagatt
     7201 gcaaatttga aaagagtaca ggaggagatg ctgttataaa tgacaaaaac atgatagaga
     7261 taggaagaga aagtgcttgc aaaatacttg gagctgaaaa tgttgaaata attgattttc
     7321 ctgcaatgac aggtgaggat tttgctattt atatgaaaga aaaacctggt ttatttatgt
     7381 atataggtgt aggagataaa gaaaaaaata taaattacag attgcatagt aacaagttta
     7441 atatagatga aaaatgtctt agtatagcac cttcattatt ttcacaactt acagtagatt
     7501 atttatgtca actatagagt acattcataa aaatatattt agcctatttt taaatttaat
     7561 gctagtatat taattttgat gtagaacata ttatttatta tatttattta aaaaggtgga
     7621 taacaatgat aaaagtagaa aatttacata aaagttttaa agggacagaa gttcttaaag
     7681 gtataaattt agagataagt caaagtgaag tagtagctat acttggatca tctggaacag
     7741 gtaaatctac gttacttaga tgtttaaatt atttagttga gcctacaaaa ggaaagataa
     7801 tgattggtga tgtaaaagta gatgtagaaa aagcaacaaa aaaagatata accaatttaa
     7861 gaaaacatac atctatggtg tttcaaaatt ataacttatt taaaaataaa acagcattag
     7921 aaaatgtaat ggaggcattg atagtagttc ataaaaagag taagaaagaa gctagagaaa
     7981 tatctttaaa attaatagat aaagtaggta tgttggatag gaaagatttt tatccatcaa
     8041 agttgtctgg gggacagcaa caaaggatag gtatagcgag agcgatggca gtaaatccaa
     8101 atgtaatatt atttgatgag cctacatctg cacttgaccc agaacttgtt ggagaagttt
     8161 taagcgttat aaaatctctt gcaaaagaag gtataacgat gcttattgta actcacgaaa
     8221 taaattttgc aaaagaagtt gctgataaaa ttatatttat ggataatggt gtaatagctg
     8281 aagaaggaac tcctgatgaa atatttaata atcctaaaaa tgaaagaaca gcaaaatttt
     8341 taaatatcat taggaaagaa gaagtattta gttaaaagtg tataaattaa gcattagaag
     8401 atatgtaaga tacccccatt aaatttataa gttagataat a
//
LOCUS       DAEOSP010000160         8323 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.163, whole genome shotgun sequence.
ACCESSION   DAEOSP010000160 DAEOSP010000000
VERSION     DAEOSP010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8323
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.163"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            123..1004
                     /locus_tag="KRH54_002825"
     CDS             123..1004
                     /locus_tag="KRH54_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBG8580463.1"
                     /translation="MVKKRKKKTVALTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(1065..2273)
                     /locus_tag="KRH54_002826"
     CDS             complement(1065..2273)
                     /locus_tag="KRH54_002826"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBG8580464.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMDLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(2346..2598)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2750..3739)
                     /locus_tag="KRH54_002827"
     CDS             complement(2750..3739)
                     /locus_tag="KRH54_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBG8580465.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRNLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVIGLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            3891..4331
                     /locus_tag="KRH54_002828"
     CDS             3891..4331
                     /locus_tag="KRH54_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8580466.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            4658..4885
                     /locus_tag="KRH54_002829"
     CDS             4658..4885
                     /locus_tag="KRH54_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBG8580467.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            4911..7031
                     /gene="feoB"
                     /locus_tag="KRH54_002830"
     CDS             4911..7031
                     /gene="feoB"
                     /locus_tag="KRH54_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBG8580468.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(7164..7982)
                     /locus_tag="KRH54_002831"
     CDS             complement(7164..7982)
                     /locus_tag="KRH54_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8580469.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
BASE COUNT         2938 a         1202 c         1089 g         3094 t
ORIGIN      
        1 gtcatataag ttacacttta gttatcttag ttacaacttt gtcggtttac tttttctact
       61 ttcttcgata taattataat atatttttga tacaattcga caaaaggggg aatgctgtaa
      121 ttatggtaaa aaaaagaaaa aagaaaactg ttgcacttac tatacttgtt gtaatagtta
      181 ttggtatttt ttctatggca tgggcggaca tgtctagaaa aaaagaaatg aaagaaactc
      241 aagaaagact taaagctgaa agattagaaa aagaaagaat tgaaaaagaa aaaaatgaac
      301 ctattattgg tgcaaaaaaa gaagctgtaa aagaatatgg atatgacgca aaaatagttt
      361 gggacaaact taacaaatac gactattcaa acaatggaga aaagatagtc tttcttacat
      421 ttgatgatgg accatctact actaatactc cacaagttct agatatcttg aaaagacatg
      481 gtgttagagg aactttcttt attaagggtg attctcttga aagaaaagga gctaatgaaa
      541 ttttaaaaag aacatttgac gaaggaaatg caatagccca tcactcttat actcatgact
      601 ataaaaaact atatcctaac agaagcctaa atctcgatac attcgtaaat gaacttaaca
      661 aaactgatga agctatgaaa aaagtcttag gaaagaattt ttcctcaaat gtagttagat
      721 gcccaggcgg gtatatgtca tggaaaaata tggaacctct aggaaactac ttaaaagaga
      781 aaaacatggc atcaattgat tggaacgctt taaatgctga tgcagaaggt aaaaagaaaa
      841 atgcacaaga attatttgaa catgcaaaga aatcttctga aggcaaagaa atggttgttc
      901 ttttaatgca tgatacttat ggaaaacaag aaacagtaaa tgcattggac caaattataa
      961 catattttaa agacaatggt tatcaattta aaattttaat ataataaaaa agcaagttta
     1021 ttgaatgtac attttatact caatagactt gcttttactt tactctattt tactagctcg
     1081 atttttacaa attttttctt tcctatttga acaactaatt cactctcttt tacaatagtg
     1141 cttaaatcct ctactttttc accatttacc tttactccac cttgagaagc taatcttcta
     1201 acttcactct tactcttaac aatctcatta gaaactagta cttcgattaa gtcaaatcca
     1261 tcttccttaa cttgaattgt ttgtatatct tcaggtattt gacctttttg gaatacagat
     1321 ttaaatcttt cttcagcttc tttagcagat tcttctccat ggtataatgt tactatttct
     1381 cttgctaagt ccatctttat atctcttgga tttactgaac cctctttcaa ctgagattct
     1441 attttattaa cttcatcagg atgaacatct gtcactaaat tgtagtattt aattataagt
     1501 tcatcaggaa tttccataga tttttggtac attattcccg cttcttcatc tattccaata
     1561 tagttaccta aactcttact cattttttct ttaccatcta atccttctat taaaggcatc
     1621 attattacta tttgagactc cataccaaat tctctttgta atgatcttcc cattagtaaa
     1681 ttgaatcttt ggtcagttcc accaagttct atatcagctt ctaatgctat agagtcatat
     1741 gcttgcatta atgggtagaa gaactcatgt actgatattg gcatttgtcc ttcatatctc
     1801 tttttaaaat cttctctttc taacattctc gcaacagtaa tagttgctgc taattttatt
     1861 acatcttcaa aatttaactt agctaaccac tcgctattaa atcttacaat agttttttct
     1921 ttatctaaaa ctttgaaaat ttgttcttca taagtttttg catttgcaag aacttgctct
     1981 gttgttaaag cttttctagc tttagatttt ccagttgggt ctccaatttt tcctgtaaaa
     2041 tctccaatta ttattactat ttgatgacct aaatcttgta attgtttcat ctttctaaga
     2101 actactgtat gccctaaatg tatatctgga gcacttgggt ctagacccaa ttttactatc
     2161 ataggttttc cttctttttc tgatttaata agcttttccc ttaactcttt ctcatctatt
     2221 aaatcatcta cacctttcat aattattcgc atttgctcgt ctatcgattt catttttcat
     2281 tcctcctaaa atatttatat aatctataat ttttattttt ataataaaaa aagctttcat
     2341 cctcttaaaa ggacgaaagc attgcttacg tgttaccacc ttagttcatc aatatctcac
     2401 gatattaatc tcttagagta caattacata tactcgtgta ctataacgtg tactaatccg
     2461 atatctctta atggttgtta tctttcagag atagtgctct gagatgtatt caaactaaat
     2521 caatttattc catttcacca aactggaact ctctgttaaa taaaatctag ccctacttct
     2581 tctcttcata gcatttatat attcagatta tttcatcaat tacaagaaat ttttcttaac
     2641 ttactcataa tatattatta tttttatatt ttgtcaacct tttttattat tatatacatg
     2701 tttttttaca ttatactact tttttatatt ttttaccaat acccaaaatt tatttttcaa
     2761 tcaaaagatt tatttgttta ttgactatat tactctgact caatcttata tttattttct
     2821 tatttaaaaa tgcgtttata attattaaac caataactgt tacatcaaat ataaaaaaca
     2881 gcaataaaat tattatcaaa aataaaattc tagcatataa taattttcta aacttagcta
     2941 ttataaagtt ttgtagtgac atacatgagt ctctaatgat aaaatagaca cttatatagg
     3001 ttattgatat aaagattgtt tttaaataaa cagaatcaac tttaacattc aacagtttta
     3061 gaacttcaaa aatggctaga taagaaacac atatgtaaaa agcttttttt gagcaacata
     3121 aaaattggtt taaatttcta aaattcttaa atattaaaaa tttttttaaa gaattagaat
     3181 ctaaaatatg caatttattc cctaaaatta aacttaataa atatatgcaa aacaccatac
     3241 caaaaggaaa caatgctatc attaaataat aaataaaatt tgtataccca ttaaaataaa
     3301 tccatttgct atatccttga aattctttca taaaactata acctattaat gttgaagcat
     3361 atatatcaat gaaaaccatt acaataaccc caaatactga accatatact aaatcatcca
     3421 taaccattgt aggcttattt attagatagc cacacattat accaagtaat acactctgac
     3481 ttgcccaaat agcagttcct aaatttccaa gtaccagaga aattattaaa agcgaagtaa
     3541 ttccagataa aatggtatac tttaaatcac atttcaagca gattatacaa aaaaatactg
     3601 gcactataaa atctaagtaa atagcatatc caaatccagt actcacagct ataattgtta
     3661 ctactatgaa taaagatgac aataaagctg cttcagttaa ttttttagta gtattcaaaa
     3721 cactgcctcc ataaatcata aattttctaa ctaaagtttt gtttttaata tagtatatca
     3781 ctttttaaaa atatttttaa taaatataaa ttttaataaa ggtatttatg ttaaaaatgg
     3841 tataatgtat cttaatattc agttttttaa gatgctagga ggtaattact atgtttaaaa
     3901 ttaaacactt taatgatttg agtttagatg aattttatga aatagcaaaa tcaagatatg
     3961 aggtatttgc ttgtgaacag aagatatttt cattaaacga ttatgatgat atagataaaa
     4021 gctcttatca catattttta aaagaaaatg gattgatttg tgcttacgct agaataattc
     4081 ctaaagaata cagctcttat aatgatgttt ccattggtag agttttagtt ctttctagtc
     4141 atagaagaaa aggattagca aaacaaatga tggattgtgc tatagatttt ataaaagtta
     4201 acttacacga aaataatata accttatcag cacaaactta tataaagaac ctttacttat
     4261 cttgtggttt taaagaaatc tcagaagtat acgacgaagc aggtattgaa catattaaaa
     4321 tgagattata gtgttgacaa gctaatattg ttaatatata atttaagtat aaattaaata
     4381 ttgaattgat atttaggtat ttgaaaatac cttacataga gtcagaaggc ttttgctttc
     4441 tgactttttt attatcaaaa ttcaataatt atatggattc aaagtgttaa tttttattct
     4501 atattctact ttccccaaaa gtaagaatta tacccaactt tgtatgatag gcatcttgcc
     4561 tatctttttt ttgtaaaagt aacaaggtaa taatataatg catagtttaa aataagaaga
     4621 ttagcccagg ctaaatttca agcatggagg tgctattatg aaaaacctta acgatattca
     4681 agtgaaaaaa actgtcaaag ttgaagatat attatctagt ggtaatttaa gagaaagaat
     4741 gttagcgctt ggtcttacta gaggagctgt tatagatgta gttagaaaag gtccaaaaaa
     4801 taatttaact gtctataaaa tcagaggtag caagatagct ctacgacaag aagaaagtag
     4861 cttaatatta gtttctgaca tttaaaaatt tctagcagga ggatattaat atggggttga
     4921 cacataactc aactaaaatg agttctttaa aagacatgtt tgatatagac aataaggaag
     4981 accagtttgt aattgcattg gctggcaatc caaatacagg taaaagtact gtatttaatc
     5041 atcttacagg gcttagacag catactggaa actggccagg aaaaactgta gctacagctc
     5101 gtggtaattt taaatataaa aataccgaat atgcattaat agatttacca ggaacatatt
     5161 ctttatttgc cttatcacaa gaagaaattg tagctcgtga ttttatatgt tttggtaatc
     5221 cagatgcagt aatagtagta tgtgatgcta catgtcttga aagaaattta aatttagtat
     5281 ttcaagtgat ggaacttaca gataaagtta ttttgtgtat taatttaata gatgaagcta
     5341 gaaaaaaagg cattactatt gataaaaaac ttttagagga ttctcttggt attccagtta
     5401 ttttaactgc tgcaagaaat ggctctggta tggatgaact attggatact ttaaatgatg
     5461 tatcttttga caaatataaa ttaaataata aacctgttag atacaatgaa aatatagaga
     5521 atgtggtaaa atcaattcaa ccagaattgg ataatattat accaggtata aattctagat
     5581 ggttgggact tagacttatt gatggcgatg aaagtatatt tgaatctatg agtaattata
     5641 tagacaaaga ttctatagat gctattaatg aagtaaagaa aaagattcca gacaatataa
     5701 ataaacagaa aataagagat gagtttacta aaatcaacta tgattatgct aaaaaactga
     5761 gtgatgagtg ttgttctaat gttgctaaaa aatcaactga tagagaagaa aaagtagata
     5821 aaatattaac atctaaaata tttgggcttc caataatgct cttattatta ggtactatat
     5881 tgtggattac tatagaagga gctaactatc catccacttt attatctaat ttattactcg
     5941 gatttgagcc aagtatttcg ggtatattaa atagtattaa ttgtccatca tggttaaatg
     6001 atatgttggt tttaggatta tacagaacat tagcatgggt aatctctgta atgctacctc
     6061 ctatggcaat attttttcca ttatttaccc tacttgagga ttttggatat ttgccaagag
     6121 ttgcatttaa cttagaccac ctatttaaaa aagcttgtgc acatggaaaa cagtgtttga
     6181 ctatgtgtat gggatttgga tgtaatgcag ctggagttat tggttgtagg ataattgact
     6241 ctccaaggga aagacttata gctatactta caaacaactt tgttccttgt aatggtcgtt
     6301 ttcctacttt gatagctata tctacaatat tttttagctc agttataacc aactctttcg
     6361 tatcaagtgt agcaactgca ctttgcataa ctttactgat aattctaggt gttataataa
     6421 cattgttggt ttcttatact ctttctaaaa cgctattaaa aggagttccg tctacattta
     6481 cattagaact tcctccttat agagttcctc aaataggaag aactttatat acctctataa
     6541 tagatagaac aatatttgtt ttaggaaggg ctgtaatggt agccatacct gctggtgtca
     6601 taacttggat atttgcaaat atatatatag gagatttaag tatattatca catgttgcaa
     6661 actttttaga ccctttagct aaattaattg gtcttgatgg ttttatatta cttgctttta
     6721 ttcttggatt cccagcaaat gagatagttg tacctatact tttaatggct tacctagcaa
     6781 ctggttctat gatagaatta gatagcttta gtgctttagg tcaagttctt agagaacatg
     6841 gatggactta tttgacagct ttaaatgtta tgctgttcag tttactgcat tggccttgtg
     6901 caacaacact tttaacaata aaaaaagaaa ctggtagttt aaaatggact gccttaggat
     6961 tcttaatgcc tactatactt gcttttgttg tttgtttttt aacaactaca gtttacaatt
     7021 tatttatata attagtatta actgacagta gacatatatt agagaaacta tttaaaaatt
     7081 tatatacttt attataaagc atagagatat attcaatgat aagtaaaatt acttaacatt
     7141 tgaatatatc tctattttaa ttcttatctg gatacttgga ttgaaatttc agtcatataa
     7201 ttatcttcat tactttcaca aaaaaagtca agtatactct cctcatacga atttccaaca
     7261 attttatatt cattttcata tatatatttt ttcaattttt cgtaagattt atatgaatcc
     7321 tcatattttc caatatgttt tatgcaagca tacattcctt ctggctttac atatagcttt
     7381 ttatccttta tttttttatc tatcttcaaa taaaaacttg atatacttaa atagtcccca
     7441 ttttttatat tttcattact tactattact cccaactcat atccttgata caaattttta
     7501 gatttacaat aattggtgaa atctattaat tccatcataa aatcactatt actcatatta
     7561 ctcaaatcgc ttgatagaac taaatattct tcaaactttt tggatatata aatttcttca
     7621 ctattcattt tagaaattcc ttcaactgtt atatttattc tattttgcat cttagaactt
     7681 atctttctta gtttttttat tttttctctt atcttttttt cttcttcttt aagcattttc
     7741 acacaaagct ggggagttct attttgtata tactctttta tttcctctaa actcattcct
     7801 atttctctca atgattgtaa tatatcaagt gtatcatatt gataaactga gtaatatcta
     7861 taattatttt tatctttata ttcaggagaa aaaataccta ttttgtcata gaaaatcaat
     7921 gtctgcttag atataccaca caatttagca aattcacctg ttgtaaaata ctgtttttcc
     7981 aagggttata cactcctttt ctttttgttg tctatatagt tactatattc tttattattt
     8041 actaaatcaa gtagttatta ttttagaaaa tcaatatgat tttgatttta aagcttaaat
     8101 atcagttaga tgtcagttat aaatatttta tccattccca tcataaaaaa gatatcccct
     8161 cttaactttt ggatggaata ttatatttat acttgttaga gtgaaaattg taataggtgc
     8221 tacaataaga tatttaatta aacataaagt taattattgt aataaaaaat ctttagataa
     8281 attagagtta aagtatggaa atatagatag agaaaaagct gtt
//
LOCUS       DAEOSP010000161         8314 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.164, whole genome shotgun sequence.
ACCESSION   DAEOSP010000161 DAEOSP010000000
VERSION     DAEOSP010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8314
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.164"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            95..250
                     /locus_tag="KRH54_002832"
     CDS             95..250
                     /locus_tag="KRH54_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580470.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            380..1393
                     /gene="aroF"
                     /locus_tag="KRH54_002833"
     CDS             380..1393
                     /gene="aroF"
                     /locus_tag="KRH54_002833"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBG8580471.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1415..2479
                     /locus_tag="KRH54_002834"
     CDS             1415..2479
                     /locus_tag="KRH54_002834"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBG8580472.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFDKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2479..3792
                     /gene="aroA"
                     /locus_tag="KRH54_002835"
     CDS             2479..3792
                     /gene="aroA"
                     /locus_tag="KRH54_002835"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBG8580473.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGIKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3770..4849
                     /gene="aroC"
                     /locus_tag="KRH54_002836"
     CDS             3770..4849
                     /gene="aroC"
                     /locus_tag="KRH54_002836"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBG8580474.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4852..6048
                     /gene="pheA"
                     /locus_tag="KRH54_002837"
     CDS             4852..6048
                     /gene="pheA"
                     /locus_tag="KRH54_002837"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBG8580475.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNRYKSLKDELNKKNILVGFSGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNSKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            6095..6904
                     /gene="aroE"
                     /locus_tag="KRH54_002838"
     CDS             6095..6904
                     /gene="aroE"
                     /locus_tag="KRH54_002838"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBG8580476.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            6916..7449
                     /locus_tag="KRH54_002839"
     CDS             6916..7449
                     /locus_tag="KRH54_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBG8580477.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            7470..8309
                     /locus_tag="KRH54_002840"
     CDS             7470..8309
                     /locus_tag="KRH54_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBG8580478.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKSK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
BASE COUNT         3409 a          828 c         1507 g         2570 t
ORIGIN      
        1 gaaaatattt atagaaaata ttgacaatta tattttcttt gttataatta ttaaacaaga
       61 agaataataa attataaaaa tggaggataa tactatgaaa ggaatattaa aagaactaac
      121 agcaaaactt tatagctttt tttactttat gttctggggc ttttatttta gcgctggcta
      181 tttcttttac aaaaatatat gtacttcctt tctggtgagg ccttatttta gtgattttga
      241 gcataagtaa attttataaa aatggttatt ttatatattt atataagatt tagtgcttga
      301 tagattattt tagagaactc attagtgagt tctctttttt attttaaata taaaattata
      361 atattgggag gtagtaaaaa tgatagtagt attaaaaatg ggagcagaca aaaatgaagt
      421 taaaaaatta attgaggcta ttgggagaga aggagtagaa gttaatccca tagatggaac
      481 agaattaact gttttaggtt tagttggaga tactagtaaa atagatgcca aaagaataga
      541 agcaaataag gtagttgaaa aagttatgca tgtggtagag ccttttaaaa aggccaatag
      601 aaaatttcat ccagaacctt caatcataaa tgttaatgga atggaaattg gttcaaagaa
      661 aattgctatg atagctgggc cttgttctgt tgaaacagaa gaccaaatag tatctatagc
      721 taaagatgtg aaaaaatcag gtgcaggatt tttgagaggt ggagcattca aaccaagaac
      781 atcaccatac gctttccaag gattgaaata tgatggattg gatttattaa aaaaagcaaa
      841 agaaaaaaca ggacttccta tagttacaga aataatgtca acacaagata tagatatatt
      901 tgaagaaaat gtagatgtaa ttcaagttgg agctagaaac atgcaaaact ttgatttatt
      961 aaaagaatta ggtaaaacaa ataaaacaat acttttaaaa agaggattat ctgctacaat
     1021 tgaagagtgg ctaatgtcag cagagtatat aatggctgga ggaaatgaaa atgtagttct
     1081 ttgtgaaaga ggtataagaa cttttgaaac atatactaga aatactttgg atttaagtgc
     1141 aattttagct gtgaagaaac ttagtcattt accagttata gttgacccaa gtcatgcagc
     1201 aggtaaatca tggatggttg actctttatc taaggcagca attgcagttg gagcagatgg
     1261 tctaataata gaagttcata atgacccagc acatgcactt tgtgatggaa aacaatcgat
     1321 taagccaaat gaatatgatg aattgataag tgaattaaag actatcgcat cagcagttgg
     1381 tagagaaata taatctataa aaggatggtt aaacgtggaa gaaaaactat tagtaaattt
     1441 aaaagaaaat agctacaata tatttattaa aaaaaatata ctaaagaata taggagaaga
     1501 aataaaaaaa atatattttg gaaataagat ttttattgta actgatgaaa atatagcaaa
     1561 attatacttg gataaagtaa aaaataattt aagtgaatct ggatttgaaa ttaaatcttt
     1621 aatattaaaa tctggtgaag aaacaaaatc atttaatact cttcctaaaa tatacgatag
     1681 tttacttgac tttaagttaa ctagaaaaga tttgattatt actttaggag gaggggtaat
     1741 aggtgattta ggtggatttg cagcagcaac ttttttaaga ggtataagtt ttattcaaat
     1801 tccaacatct ttattagcac aagttgatag ttctgtaggt ggaaaagttg gagttgacct
     1861 tgagagaggc aaaaatttag tgggaagctt ttaccaacca aaggctgtgt ttatcgaccc
     1921 aaatgtatta ttatcacttc cagaaaaatt ctatagagat ggaatggcag aagttataaa
     1981 atatggatgt ataaaagaca aaactttttt cgataaactt ttaagtttaa aaaatagaga
     2041 agaagtaatg caacatatag aatacattat acataaatgt tgctttatta aaaaaactgt
     2101 agttgaagct gatgaaaaag actttggaga tagaatgctt ctaaactttg gccatacatt
     2161 aggtcatgct attgagaaat actacaattt tacgggttat acccatggag aagctgttgc
     2221 cataggaatg tataagataa ctttagaaag tgaaaagata ggaattacaa aaaaagggac
     2281 atcagaactt ataaaatcaa ttttaatgaa ttataatttg ccttatgaga ttgaattaag
     2341 agataataaa tctatagtag atgctatatc tcttgataaa aaaaatctag gtgatacatt
     2401 aaaaataata cttttaaaag atattggaga gagtattata tataatacta cttctaaatt
     2461 ttttcaggag gtaagataat gggaagtcta aaaatatatc caagtaaatt atctggagat
     2521 gtaaaaatac caccatcaaa aagtatggca catagggcag ttatttgttc atctctaagc
     2581 aatggaaaaa gtagaatttc taatatagat ttttctgatg atataatagc tactataaga
     2641 gcaatgacat cattaggagc tattatagaa aagaaagaag atattttaga aatttcgggg
     2701 attttttcta aagaaggtat tttaaataga gagaatcagt tgaatcaacc taaattgaca
     2761 atagattgta atgaatcagg ctctacattg agatttttag tacctatatc tttagcattt
     2821 gatggtataa aaaggtttat aggtagaggt aatcttggaa aaagaccact tgatacttat
     2881 tatgagatat ttgacagaca gaatataaaa tatagttaca aagaaaatca gttagatttg
     2941 attatatcag gtaagcttaa accagatgaa tttagagtaa aaggaaatat aagttctcag
     3001 tttatcacag gtctactgtt tatattgcct acgctagaga gtgattcaaa gataataatt
     3061 actactgagc tagaatctaa aggatattta gatttaactc tttcaacgat aaaagatttt
     3121 ggggtagaaa ttataaacaa taactataaa gaatttatta taaaaggaaa tcaaacgtat
     3181 aaagcgagag attataaagt agaaggagat tattctcaag gagcatttta tttatcagca
     3241 gatgctatag gagaagatat tagcatatta gaccttaaag aagattctct tcaaggggat
     3301 agtgaagtag ttgaaatttt atctaggatg ggaatggaaa tattaagaga aggcaacaaa
     3361 ataaaaggaa taacaaatgg tttaaatggt actttgatag atgcatctca atgcccagat
     3421 ataatacctg tattatctgt agtagcatcc ttaagtatag ggaggactac aataattaat
     3481 gctggtaggc ttagaattaa agaatgtgat agacttcatg caataaatgt tgagttaagt
     3541 aaacttggag caaatataga ggagaaagag gatagtttga taatcgaagg agtaagcaag
     3601 ttaaatggtg gagtagaggt gtggagtcac aaagaccata gaattgctat gacacttgca
     3661 attgcttctt gtagatgtga taaaccaata atattaaaag attttgagtg tgtatcaaaa
     3721 tcttatccac atttttttaa agattttaag atgttaggag gtcgaattga tgagtggaat
     3781 atggggaaat aatttaaaag tatcaatttt tggagaatct catggaaatg ctataggtat
     3841 aaatatagat ggtcttcctt cggggattga acttgacctt gataatattg ataaagaaat
     3901 gaagagaaga gcacctggaa aaaattctat atctacttca agaaatgaaa gtgatatacc
     3961 agaaattcta agtggatatt ttaatggaag aactacagga acgccattat gtgcaatcat
     4021 aagaaatagt gatactcgtt caaaagatta tggagagctt aaaaatttaa tgagaccagg
     4081 acatgcagat ttcacaggta atgtgagata ttctggattt aatgactata gagggggagg
     4141 tcatttttca ggtaggataa cagctccatt agtattttgt ggggctatat gtaaacaaat
     4201 tttatcacaa aaaggaatag agataggagc acacataaaa aaaattaaaa atatagaaga
     4261 tatgagtttt gattatgtaa atatttcaaa acaacagtta tcaaatttgc aaactttgga
     4321 actaccttta cttgatttat caaaggaaga agccatgaaa aatacgatta tagatgcaaa
     4381 aaatcaagga gattcagttg gtggtataat tgagtgtgca gtagtaggta ttaatgtagg
     4441 gcttggaaat ccattttttg actctgttga atctacttta tcacatttat tgttttctgt
     4501 tcctgctgta aaaggtgtag agtttggttt aggttttgaa cttgcagata tgtatgggtc
     4561 tcaaagtaat gatgaaatgt attatgaagg aaatcaagtt aagagcaaaa caaataacaa
     4621 tggaggaatt ataggaggga ttactactgg aatgccaata attttcaaag tggcaattaa
     4681 gccaactcca tcaattagca gacaacaaaa cacagttaat ataaaagata aaaaggacga
     4741 tattttatat attaaaggta gacatgaccc ttgtatagtt caaagggcta tacctgtaat
     4801 agaagcagta acagccattg gaatttttga tttgatgaaa ggacgataat aatggaggat
     4861 ttaacgagat atagaaatga gattgacaat atagataaag agcttattca attatttgaa
     4921 aagagaatga atacagttct tgaaattgcc agacataaaa tgaaaaataa cactactata
     4981 cttcaaaaag atagagaaga aaaagtacta agtagagctg tagataattt agataataaa
     5041 gcatattcac aagaaacggt acagtttttt aattctataa tggaaattag tagaggatta
     5101 caaaaaagat tgatagataa tgatggtgag cagaaaatcg agtttagttt agattcatca
     5161 aataagaaat ttgatttgtc aaatgtaaat agatataagt ctttaaaaga tgaattaaat
     5221 aaaaaaaata tcctagtagg gttttcagga aaatcaggtt catttacaga agaagcacta
     5281 aataaattct tcaacaaaaa aatatcaaaa aaacaattta aagaatttga agatgttttc
     5341 atagcactta aaaataaaga aataaactat ggaataatcc ccatagaaaa ttcatctaca
     5401 ggagctatct ctgaaactta tgaccttttg agaaagtacg gtttttatat agttggagaa
     5461 gaatgtataa aaatagacca aaatttaata gggataaaag acacaaagct tgaagatata
     5521 aaagaaatat attctcatcc acaaggtata ggacaatgta gtgaattttt aaaacaaaat
     5581 agtacatgga aattgatacc atttaataac acagcaacaa gtgcagaact tgtaaggaaa
     5641 cttcaagata aaacaaaagc agcaattgca agcaaaaaag ctgcaagtat ttatggatta
     5701 gaaataataa gtccttgtat aaatgatatt gcaaacaact atacaagatt tgtagtaatc
     5761 agtaatcaaa tacacattga agaagaatca aacaaaatga gtgtagtatt ttctgttgaa
     5821 catgaagcag gaaaacttta taaagtactt ggatactttg ctaaaaataa tataaatatg
     5881 acaaaaatag aatcaagacc tatgaaaaat gcctcatgga gatatttctt ttatatagat
     5941 tttgagtgtt ctatatacaa ttctaaagtg tacgatttat tagaattgat agaacataat
     6001 acagcatatt ttaaatttat gggagtatat aaaaataaaa ctgaatagat aagataaatt
     6061 tttatttaat ttataattat taggaggtgt aagtatgaat tttttcggtc tagtaggtga
     6121 aaaactctct cacagtgttt cacctcaaat ccacaagaga gtattcgaga tattgaatat
     6181 tgaaagtgct tataaaaact ttgaaatttc aaaagaagat atatctaagc tagacggtgc
     6241 tataaaatta ttaggaatac aaggagtaaa tgttactgta ccatataagg agcgtattat
     6301 gaaatatctt gattttatat caccagaggc aaaacgtata ggagcagtta atacaatttt
     6361 actcagagaa aatatgttat atggttacaa tacagattac tttggtcttg atagtatgtt
     6421 taaaatggct aatattgatg ttcaaggaaa agtagcagtt atattaggaa ctggaggtgc
     6481 ttctaaagct gctcttacat attttattga ttctggtata gaaaagcttt atgtttcaac
     6541 tagaaaaaag gatgataaaa aattattaaa tagtaaagct atattaatag attatgaaga
     6601 acttaaacat ataaaaggag atataatatt aaatgctaca ccagtaggta tgtaccctaa
     6661 tgtaggaatt tcaccagtgt caaaatctat tatacaaaat tttgatatat taatagattt
     6721 aatttacaat cctggagaaa cagagttttt aagaattggt aatagtatgg gtaaaaaaac
     6781 ttgtgatgga ttatatatgc tggtggggca agcaataaaa tctcaggaaa tttggcaaga
     6841 tactaaaata gataatagta ttttagatgt tatatataat gaactaaaat tagaattttt
     6901 gtaagggaga ttgttatgat aaataaaaca aaagaaaaat tgatattaat aggtatgcca
     6961 ggtagtggaa aaactacaat tggtaaactt ttggccaaag aatataattg tagtttctgt
     7021 gatatggatg attatataat tcaaatatct caaaaaagta tagcagagtt gttttcagaa
     7081 ggagaagata tctttaggaa ctatgagact caagcatgta gagaactttc tataagtgat
     7141 aaaacagtga tatctacagg tggaggtgta ataaaaaagg atgttaatat ggaaatacta
     7201 aaagaaacag gtataataat ttttattgat agacctatac aaaagatttt ggaagatata
     7261 aatattaatt caagaccatt attaaaaaac ggaaaagata gattgtataa tctttacaat
     7321 gagagaataa atctttataa aaaattttca gatatagaga ttttaaatga taaatcatta
     7381 aataatgctg tatataatat tacaaatgca gtcagtgaaa attttaaatt tgactttaaa
     7441 gaaaaataat ctagggggaa gtaaaaataa tgaatattgt tattgtagga ctaggagtta
     7501 taggaggctc ttttgctaaa gcattaaaaa aagctggata tgaaaatgta tttggagtag
     7561 atgtagattt agaaacttta aaaaaagcag aaaaatcaaa aatcataaaa aaaggatgta
     7621 ctacaggtaa agagttattt aaaaaagctg atttaattat actatctata tatcctagat
     7681 tagttgtaga ttttctaaat aataataaaa acttttttaa aaaagggact ataattactg
     7741 atactacagg aataaaagaa acacttatca atgatgtgtt gcaaattatt ccagatgata
     7801 tagattttat atttggacat ccaatggcag gtagggaaaa aaagggtatt gattttgcaa
     7861 gtgaacaagt ttttaatggc gcaaattata taattactcc aactggcaga aacaacataa
     7921 aaaatttaga gttagttgaa aatttaattt tagagattgg atttaaaaga gtaaaaaagt
     7981 taacttcaca aaaacatgat gagattatag cttttacttc tcagttacct catgttatgg
     8041 cagttgctct tataaatagt gatgaagaag gtagagatac gggtaaattt attggagata
     8101 gttatagaga tttaactaga attgcaaata tgaatgaaga cttatggagt gaactttttt
     8161 taggaaatag agataattta ctaaaagtta tagaaaactt tgaatcagaa gtaaatttag
     8221 taaaagaagc tatatttaac aatgataaaa ataaattaat agagtatttt aaaaaatcat
     8281 caataagaag agaagcttta gaaaaataaa tatt
//
LOCUS       DAEOSP010000162         8252 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.165, whole genome shotgun sequence.
ACCESSION   DAEOSP010000162 DAEOSP010000000
VERSION     DAEOSP010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.165"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            50..805
                     /locus_tag="KRH54_002841"
     CDS             50..805
                     /locus_tag="KRH54_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8580479.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            936..1958
                     /locus_tag="KRH54_002842"
     CDS             936..1958
                     /locus_tag="KRH54_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG8580480.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(2122..2679)
                     /locus_tag="KRH54_002843"
     CDS             complement(2122..2679)
                     /locus_tag="KRH54_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG8580481.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPIKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2685..3761)
                     /locus_tag="KRH54_002844"
     CDS             complement(2685..3761)
                     /locus_tag="KRH54_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBG8580482.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(3762..4445)
                     /gene="modB"
                     /locus_tag="KRH54_002845"
     CDS             complement(3762..4445)
                     /gene="modB"
                     /locus_tag="KRH54_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBG8580483.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWSGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4594..5406)
                     /gene="modA"
                     /locus_tag="KRH54_002846"
     CDS             complement(4594..5406)
                     /gene="modA"
                     /locus_tag="KRH54_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG8580484.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5599..6528
                     /locus_tag="KRH54_002847"
     CDS             5599..6528
                     /locus_tag="KRH54_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8580485.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(6689..7138)
                     /locus_tag="KRH54_002848"
     CDS             complement(6689..7138)
                     /locus_tag="KRH54_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580486.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPNIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7341..>8252)
                     /locus_tag="KRH54_002849"
     CDS             complement(7341..>8252)
                     /locus_tag="KRH54_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8580487.1"
                     /translation="LILLIVSYIFGKLVYNSSVGLEQLIKNEDIVKIFSERKDKPLEK
                     LKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMILVHGIGSSYYEMLKVAYRYLDKG
                     YNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKFVKNKFPEGRLGVHGFSMGAGTAA
                     MHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEKRIPDILINYVVTCGDLYNKFKSG
                     FWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNSKKMYDLVKHDKKDLWLIEGIGHV
                     DGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         2862 a         1361 c         1006 g         3023 t
ORIGIN      
        1 aaatgcaaag ctagtaccaa aaataaatca gaaaaaagag gtgttgtaga tgttacaaat
       61 taaaaatctt tcaaagagtt tccctaatcc atatggtgaa cctaatacta tctttgaaaa
      121 tttatcaatc gatatagaag atggtgaatt tgtaagtatc ataggtagta atggaactgg
      181 taaatctact ttattaaata taatatccgg gcttataaaa gaatcttctg gtcaagtatt
      241 attaaataaa cataacttgt ctaatttagc tgaacacaga agaactcaaa tagtaagtag
      301 agtttttcaa gacccaagtc ttggaacttg cccttctatg actgttagag aaaacttgtc
      361 tttagctctt aacaaaggaa aactattaaa tctaagaaaa tgtcttcgtt acaaaagaga
      421 tttcttagaa aatttattgg aaggtgtatc tcttgattta aaaaaatacc ttgatgttca
      481 agtaaaattt ttatcaggag gtcaaagaca atcattatca ctaattatgt cgtgcttaac
      541 aaatccatca gttttattac ttgatgaaca tacagcagca cttgacccaa aaacatcaaa
      601 tgaagtaatt gaattgacta ataagattgt aagggaaaaa aatataacta cacttatggt
      661 tactcacaac ttaaaacacg ctcttcatta tggtgataga ttgataatgc ttcataaggg
      721 tgaagttgta ttagatgtaa aaggtaaaga aaaagaacaa ttaacagttg aagaaatctt
      781 agaaaaattt gaatatgcag tttaaaataa tatatctatt gtattaaact cggctttaaa
      841 ataaagttaa aattacaata attatggtta ctttgtaata attcatatga atatatgaac
      901 atatataaca ataaattttt gagggggata taaaaatgat taataaaaaa agattggcaa
      961 gtttgatttt agctggtgct cttagtatat ctatgcttac agggtgttca caaggaggag
     1021 actctggtaa ttcaaaacaa gaaagtaatt ctaaaggtaa agaagttaaa aaaattggta
     1081 taactcaact ggttgaacat ccagcattag atgccactag gacaggtttt gtaaaagcat
     1141 tagaaaagaa cggatttaaa gatggagaaa atattgatat agacttccaa aatgctcaaa
     1201 atgacatgcc tactacacaa agtattgcga gtaaatttgc atctgataag aaggacttaa
     1261 tatttgctat atctactcca tcagctcagg cagcatttaa tgctactaaa gatataccta
     1321 tacttattac tgctgtgagt gaccctgttg ctgctggatt agttaaaact cttgaaaagc
     1381 caggcacaaa tgtatctgga acatcagatt ttgtttctgt agacaaaggt ttagaacttc
     1441 ttaaaatatt tgctcctaag gcaaaaacta taggagttat gtacaatact agtgaagtaa
     1501 attccaaagt tcaagttgat gctttaaaag aatatgcatc taaaaatgga tttaaagttg
     1561 ttgaaaaagg tataactact tcaaatgaag ttaaccaagg tatttctagt ttagttggca
     1621 aaatagacgt attatatgtc cctacagata acttagtggc ttcttctatg cctatagtct
     1681 ctaaaatagc tactgagaat aaaatacctg ttatagcagc tgaatctggt ccagttgaaa
     1741 aaggtgctct tgcttgtcaa ggtatcaact acgaaaaact aggttacaaa acaggtgaaa
     1801 tggcagttaa aattttaaat ggcgaatcag tatctgatat gcctgtggct acatcagatg
     1861 atacagatat cattgttaat gaagatatat taaaagcttt aggtatggaa aaaccttcta
     1921 atgaaaatat ttcttatgta aagactaaac aagaatagaa agatattttc tatatacgat
     1981 taatatgcct gtttctataa attaatttat agaaacaggc atatattttt attttaatgt
     2041 attatctatt taaagattat atgtaaatgc tcttaatttg tttgtataaa ttttacttgt
     2101 ataaacttta tacaatcata tttattcttc tattttcttt attactctac atggatttcc
     2161 aacagctaca gtatttggtg gtatatcttt tgtgactaca cttcctgcac ctattactac
     2221 attatcacct atagtaattc ctggtgttat gattactccc ccacctatcc atacattatc
     2281 acctattttt ataggagaac catactctat gcctgaattt ctaagttgtg catcaattgg
     2341 atgataggct gtatagattt gaacgtttgg agctaacatt acattgtcac ctatttcaat
     2401 tttacataca tctaaaaaaa tacaatcata atttgcaaag aaattttcgc ctacatgtat
     2461 gttatatcca taatcacacc taatattttg ttctacattt atttgcttgc caactgaacc
     2521 aaatagttgt cttaaaatat cttctctttt ttcatattca tcctctaaag tattattaaa
     2581 caatctagtt aattttttac aatactctct ttcttttact aacaattcat catttgcata
     2641 gtatcctttc ccagacaaca ttttttcttt ttcagtcata tagtttactc ctttttgtta
     2701 taattgtatc taaagcaaaa taaatattcc tgtggaaaat ctaaatatat tctgtcacct
     2761 ttcttaaatc tcattttact tttttccaat tctatttgaa taggtacaca ttcgttagat
     2821 aaatcagttt ttctaacata tatagtatat gtaaaaggat tttctacaat gttaatcaat
     2881 tcgaaacata acttttcatt taaatcttca ttagaatcca agaccttaat atgatgctcc
     2941 ctgattccca catattctat attatcacct atttctctat ttaatgtcaa ctcgcatccc
     3001 cagtctgtag cataaatagt atttttatcc agtctattta atttggatat atttttacac
     3061 cctgttatct tagcttctat aaggtacttt ggagattcaa agagctcttt tttaggtctc
     3121 tttggaagac cttctccttt gttgtacact ataatatcat cacaaactcg gtaagcttct
     3181 tcaatatcat gagtaacaaa tagtatatct cccttgtaat cttttaacat attcataagt
     3241 tctttttcca tattgcttct taaatgatgg tccaatgctg agaaaggttc atctaaaagt
     3301 aatatatctg gagatgttgc caatgctcta gctagtgcta ccctttgttg ttgaccacca
     3361 gataattgcc aagggtatct cccctcaaac ccatttaatt taagtatatc taagtacctt
     3421 gctactattt cctttttttc actcttactt agttttaaaa gcccaagttc tatattttga
     3481 cttacagtca tatgtggaaa caaagcatag ttttgaaata aaaatcctac ctttctatct
     3541 tgtgttgata aatttatctt cttttcagaa tcaaatagaa ctctatcatt taatacaatt
     3601 ttacctcttg ttggtttctc aagccctgct atacatttta atgtcatact ttttccagaa
     3661 ccagattctc ccaaaaaccc cagagtacct ttttcttgtt taatttctac ctttagttta
     3721 aaagaggata aatctttttc aatatccacg tataagctca actatccaca cctctttcct
     3781 ataattttct gctgaaattc cgaccaataa tttagtaata aaatcattgc tatagatatt
     3841 gctacaataa tcataaccca catcatagct ttattcatat ctccgccttc aacagcaaaa
     3901 aatatagcta ttggcatagt ttgagttttt ccaggtatat ttccagctat cataagtgta
     3961 gcaccaaatt ctccaagagc tctggcaaac gacagcacca atccaccaat tattccagac
     4021 catgctaatg gaactgcaat cttaaaaaat actttccatt cattaagacc taatgttcta
     4081 gcagctgaca acatattatt atcaatctgt tcaaaagctg accttgaagt tctatacatc
     4141 ataggaaaag acaccactac tgctgaaatt acagttgctg tccaactaaa tattaaagtc
     4201 ctatcgaaat ttaataaaaa ctttcctata aatccattct tcccacatag taataataaa
     4261 aagaaaccta caactgttgg tggcaaaatt agtggaagtg taaatagtcc atctataaga
     4321 cccttccact tacctctata gttagccata atatatgaca caaatattcc tattacaaaa
     4381 gttattattg ttgacaaaat agatgtctta agtgatatcc ataagggtga ccaatctgtt
     4441 cccatctttc cctccttaat tttaaatgga aatagtgaaa ctaataactc tattaatttc
     4501 actatttcca ttttattata atacacaatt attttcaatt ttcatttatt tatatataat
     4561 ttatacttaa ttacacataa tttatttcta agtttattct acttttttat atccaaattc
     4621 ttcaaaaatc ttttgtcctg actcacttaa taaaaattct tcaaatttct tagcagcatc
     4681 tacgttttta ctagctttaa ttactgaaac tggatatgta ataggcgaat gagttttttc
     4741 gtctgttttt tcaactactt ttatcttatc attattgaca gtatcactga agtaaacgaa
     4801 tccaacatca gcatttcctg attgcaccca tgctaaaact tcttttacat cttttgcaaa
     4861 tactagctta tcttttaact tatcttttaa atttaaatta gttaacacct catctgcata
     4921 cttaccagct ggaacacttt cagcttcacc aacagcaatt ttcttcactt tatctgtagt
     4981 caaatctttc atacctgata cactactgtc agcagaagaa ataagaacta aatcattttt
     5041 tactaaatct ttcatagtat cagaaactaa taacttctct tcatccaaag ctttcatttg
     5101 tttttgacct gctgaaataa ataaatcaca aggtgctcct tgttctattt gttgttgtaa
     5161 tgagcctgat gctccatagt taactgtaag ttttacattt gaatcaactt ttttatattc
     5221 ttcttcaatc tttgccattg cttcttttaa acttgcagca gccgatatat ttaactctac
     5281 tgaatcacta cttttagttg ttttttcttg ttttccatca tcttttttgt cttcattact
     5341 attgcaccca actgtcccca aagttaaaca tgcaactaat cctaatattc ccaatatttt
     5401 tttcataaca atctcccttt ttgtaaattt gatacacatt ttaacatgtt ttattatgtt
     5461 ttatcatgtt ttgttatata tagtttaatt atatatacat gataataaat tttcaagtaa
     5521 tttatttaca ctacttttaa atattttgct acattttcct ttttttaatg ttaaaataac
     5581 cttatatgga ggtggattat ggactcatta tcatctttga cacctgtcga ggtagctgaa
     5641 ttattaaaga taacaaaaaa tactgtatat gaattaatca aaagaggtga acttccttct
     5701 tataaagtag ggaaaaaaat aagagttgat actaaagatg ttgaagaata tatcaacagc
     5761 caaaaaactg gtaaagtaaa ggatacacat ttcaacaata aaaatataga tataaataat
     5821 aatttcaaaa ttattatttc tggtcaagat gtaatattag acattttagc taggtctgtt
     5881 gaaaaaaaat tggatggaat aagtacattt cgctcttata taggtagtta taatgctttg
     5941 tatgagttat ataatggcag agtttctata gcatcatccc atctatggga tttcgaaacg
     6001 gatgaatata atagcacttt tgttaaaaag ctattgccag gaataccatg tgtacttata
     6061 aatctagctt atagaatgca aggtttctat gttgctaaag gaaatcctaa agagataaca
     6121 acctgggagg atttaacaaa accaagtata accattgtaa atagggaaaa aggttctgga
     6181 actagggttt tactagatgg taaacttaga cttctaaact ttaatggtaa acatataaaa
     6241 ggttatgata atgaagaatt atcacatctt ggagttgcaa gtactgtttc tcgtggaata
     6301 gcagatgttg gtttgggaaa tgaaaaagct gctttacaag taaataatat cgactttatt
     6361 cctcttcaca aagagcgcta tgatttagtt attaaaaaag aagacttaca aaatccagtt
     6421 taccaaacta taattaacat aataaactct cctgagttta aagctgaact tcaaggtctt
     6481 ggcggatatg atttaactga tactggaaaa actatagcaa aagtttaaga ttaaaatcgc
     6541 aatctatttt ataagattta taatttttca caaaatttat aacttaaaca cgaaataaat
     6601 tgcgatttta gtgtctcaaa aaatttatgt ctttcatttt atcatccaat acaaaatata
     6661 atataattaa ttgtgcaatc atattatatt aatatatatt tacataatag tatttttctt
     6721 taatgtagat taacatatga tatgttttaa acatcaatcc ccttgctttc ttatatatat
     6781 ttggcaacac atatgagttt aataaattat atacaatgtt gctttcttct tgtatttttt
     6841 caaccaacaa gttatttata aaattacctt tctcattttc aataatctca ctccaattta
     6901 tcttaacttt taatatatat cttcccacta ggtatacaca ctctacaata aataataaaa
     6961 atacaatgca gtcagtacct gttgtcttat ataaattacc cgatactaat ggctttgcga
     7021 agaaattata aaaattcaaa gtatcacccg tataatcaaa aagtattaat agcactccta
     7081 gaactctcaa tactttaata ttcaacatgt ttttatcttt attatttttg tttttcatat
     7141 atactacctc tcttttcttt agatttgatt gtaatctaat actaactctt ttatttgagg
     7201 tataaatgaa tttaattggt agttattttc atttttacta gaaattaatt cacattacta
     7261 aacaaaactt atatttaaac ataaaaatag cctatctatc ttttaataaa tacaaaaata
     7321 aataagctac taatattcaa ttaactttta tcacttaaaa cattactatc tataaattta
     7381 aatattttat tgaagtatac tgtactatca tgttcaaatc catcaacatg accaatacct
     7441 tctataagcc ataggtcttt tttatcatgt ttaactaaat catacatttt cttgctattt
     7501 tgataattac aaacagtatc ttttgttcca tgtataaaca gtatgggtac attagatttc
     7561 tcaactgact catatggttt tacatcactg taccaaaatc cagatttaaa cttattatat
     7621 aaatctccac aagtcaccac ataattgatt aatatatctg gtattcgttt ttctagtact
     7681 cccattctaa ttgcatcttt catttcagaa taaggagaat ccagtatata aaaatcgacc
     7741 ttatcatctt ttgaatttat ctctgaatgc atagcagctg tccctgcccc catggaaaat
     7801 ccatgcactc ctaatctccc ttcaggaaat ttatttttta caaatttaac tagactatct
     7861 aaatcatacc tttcataaag accaaatgtg taattatctc caccactatt accagtattt
     7921 ctttgattat aaactaatac attataacct ttatctaaat atctatatgc tacctttaac
     7981 atttcatagt aactacttcc aattccatga acaagtatca tagtatctct tgtctttata
     8041 tttgaagtta taaaaagact ttcaagcttg taattattag aactttgtat cattaattca
     8101 gatgtcttgt agttttttaa tttctccagt ggtttatctt ttctctcaga aaatattttt
     8161 actatatctt cgttttttat taactgctct aaaccaactg aactattata cactaatttt
     8221 ccaaatatat aagatacaat taataggatt aa
//
LOCUS       DAEOSP010000163         8174 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.167, whole genome shotgun sequence.
ACCESSION   DAEOSP010000163 DAEOSP010000000
VERSION     DAEOSP010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8174
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.167"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..198
                     /locus_tag="KRH54_002850"
     CDS             <1..198
                     /locus_tag="KRH54_002850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580488.1"
                     /translation="NWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGL
                     VFNAAGVKDILLELFNRIIGA"
     gene            315..812
                     /locus_tag="KRH54_002851"
     CDS             315..812
                     /locus_tag="KRH54_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBG8580489.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            787..1293
                     /locus_tag="KRH54_002852"
     CDS             787..1293
                     /locus_tag="KRH54_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG8580490.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            1277..3724
                     /locus_tag="KRH54_002853"
     CDS             1277..3724
                     /locus_tag="KRH54_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8580491.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3727..5904
                     /locus_tag="KRH54_002854"
     CDS             3727..5904
                     /locus_tag="KRH54_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG8580492.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            5901..6902
                     /locus_tag="KRH54_002855"
     CDS             5901..6902
                     /locus_tag="KRH54_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBG8580493.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            6899..7831
                     /locus_tag="KRH54_002856"
     CDS             6899..7831
                     /locus_tag="KRH54_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBG8580494.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIVK"
BASE COUNT         2564 a         1549 c         1729 g         2332 t
ORIGIN      
        1 aactggtttc tatcaaacgc tcaatcactg gtgcttttag caatcgttgt gattggcttg
       61 tatcttggct tcaagcgtga gtttagcaaa ctgattggct ttttaattat tgcgattatt
      121 gcggttggct tagtcttcaa cgctgctgga gtaaaagaca ttttactaga gctattcaat
      181 cgcattattg gtgcttaaat aaaaccgttc ttttgtggaa tataagtggt tttcttatgt
      241 tccgcaaagg aatggtacac caaacgaagt gcggtaggga tttttgaatc tctacaaaga
      301 aaggacgtga atatatggac gatatgcaag tctatattgc gaatttaggc aaatacaatg
      361 agggcgaatt ggtcggtgcg tggtttacct ttcccattga ctttgaggaa gtcaaagaga
      421 aaatcggctt gaatgatgaa tatgaggaat acgccattca tgactacgag ttacccttta
      481 cggttgacga atacacttcc attggcgaac tcaatcgact atgggaaatg gtatcggaat
      541 tacccgaaga attacaatcg gagctatctg ctctgctcac tcatttttca agcattgaag
      601 aactaagcga acatcaagag gatattatca ttcattccga ttgtgatgat atgtatgacg
      661 tggcacgcta ctacattgaa gaaacgggtg ctttaggcga agtaccagct agtcttcaaa
      721 actatattga ttatcaagcc tatggtcggg atttagacct ttcaggaacg tttatctcaa
      781 ccaatcatgg gatttttgaa atcgtctatt aaatctgtcg gtacattact actggcagat
      841 tttctatttt acggggtggc tcaatcagct acccctattt tttatgaaag gattgattac
      901 atgaagaaaa tacgaagcta taccagtatc tggtctgtgg aaaaggtact gtattctatc
      961 aatgatttta gacttccgtt tcccataacc tttacgcaaa tgacatggtt tgtcgtgtca
     1021 ctctttgcag tgatgatact tggcaacttg ccccctcttt ccatgataga gggagcattt
     1081 ctcaaatact ttgggattcc tgtggctttc acatggttta tgtctacaaa aacttttgat
     1141 ggtaaaaagc cttatggatt tttgaagtct gtcattgctt atgcactgcg accaaagctg
     1201 acctatgcag gaaaaaaagt aacgcttggc agaaaccagc cacaagaagc cattacagca
     1261 gttaggagtg aattttatgg catatccaat taaatacatt gaaaacaatc tcgtctggaa
     1321 taaagacggg gaatgttatg cttactatga gcttgttcct tacaattact catttctaag
     1381 tccagaacag aaaatacaag tgcatgattc tttcagacag cttatcgcac aaaatcgtga
     1441 tggcaaaatt catgctttac aaatcagtac agaatccagc atacgttctg cacaagagcg
     1501 ttccaaaaat gaagtcactg gcaagctcaa agcggttgcc tatgacaaaa tcgaccaaca
     1561 gacagacgct ttaatatcca tgattggcga aaatcaagtg aactaccgtt tctttatcgg
     1621 ctttaagttg cttctcaacg atcaggagtt ttctatgaaa agtcttaccg ttgaagcaaa
     1681 aaatgctttg tctgattttg tctatgatgt gaaccataag ctgatgggcg attttgttag
     1741 tatgagtaat gatgaaatcc tgcgttttca gaagatggaa aagctcttag aaaataaaat
     1801 ctctcgtcgt ttcaaaatcc gcaggttaga taaggacgac ttcggctatc tgattgaaca
     1861 cctttacgga cagacaggca ctgcctatga agagtatgag taccatctat caaagaaaaa
     1921 gctggataat gaaacgctga ttaaatacta tgacttgatt aagcctactc gctgtttggt
     1981 ggaagaaaaa cagcgatatt tgaaaatcca gcaggaagat gaaaccgtct atgtagctta
     2041 ctttaccatt aacagcattg tcggagaact ggacttcccg tcctctgaaa tcttctacta
     2101 ccagcaacag caatttacat tcccgattga tacgtcaatg aatgtggaaa ttgtagcgaa
     2161 tcgtaaagcc ctatctactg tccgcaataa aaagaaagaa ctgaaagact tggataacca
     2221 cgcttggcaa agtgataatg aaaccagctc caatgtggcg gaagctctgg aaagtgtgaa
     2281 tgagctggaa accaatttag accaaagcaa ggaatctatg tacaagctgt cttatgtggt
     2341 aagggtatca gcaaatgatc ttgacgaact caaacgtcgt tgtaatgaag tgaaagattt
     2401 ttatgacgat ttaagcgtaa aactggtacg accatttggg gatatgctcg gcttacatga
     2461 agaattttta cctgccagca agcgttatat gaatgattat attcaatacg tgacctctga
     2521 tttcctcgct ggtttaggtt ttggtgctac tcaaatgctg ggggaaaatg aggggattta
     2581 tgttggctac agcttagata ctggacgcaa tgtctatctg aaacctgctc ttgccagtca
     2641 aggggttaag ggttcagtaa ccaatgcgtt agcgtcggct tttgttggtt cgctgggtgg
     2701 tggtaaatcc tttgcgaata accttatcgt ctattatgcg gtgctttatg gggcacaagc
     2761 agtgattgta gacccaaaag cagaacgtgg cagatggaaa gaaaccttgc cagagatttc
     2821 ccatgaaatc aatatcgtca ctctgacttc tgatgagaaa aacaaaggct tacttgaccc
     2881 ttatgtgatt atgaaaaatc ccaaagattc tgaatcactg gctattgata ttctgacatt
     2941 ccttacgggg atttcctctc gtgatgggga acgcttccca atccttagaa aagccattcg
     3001 tgcagtaacc aatagtgaag tacgagggtt gatgaaagtg attgaggaat tacgggttga
     3061 gaatacgcca ctaagtacca gtatagccga ccatatcgaa agttttacag actatgactt
     3121 tgcacattta ttattcagta atggttatgt ggagcagtct atcagcttag aaaaacaact
     3181 gaacattata caggttgcgg acttggtact tcccgacaag gaaacttcct ttgaggaata
     3241 taccactatg gagcttttat ccgttgctat gctgattgtc attagtacct ttgctttaga
     3301 ctttatccat acagaccgaa gcattttcaa gattgtagat ttagacgaag catggagctt
     3361 tttacaggta gcacaaggaa aaacactatc tatgaagctg gttcgggctg gtcgtgctat
     3421 gaacgctggg gtatatttcg tgacccaaaa tacagacgac ctcttagatg aaaaactgaa
     3481 aaataacctc ggcttaaaat ttgcatttcg ttccactgac cttaacgaga ttaaaaagac
     3541 cttagccttt tttggtgtag acccagagga cgaaaacaat cagaagcgat tgcgtgattt
     3601 ggaaaacggg caatgcctta tcagtgattt atatggtcgt gtcggtgtga tacagttcca
     3661 ccctgtattt gaagaactgc tccatgcctt tgataccaga ccacctgtgc gaaaagaggt
     3721 gtaaatgtga aaccatcaat agtaaacaga ataaaatcaa actggacgct gaaacgtcta
     3781 ggtaaagtgg caatgacagt ggctttcaca cttgtgattg ccatttttct tttagccatg
     3841 ctgggaacgg tggttcaagc tgcgggcttg gtagatgata cggtcaatgt ggcaaatgaa
     3901 tacagccgat acccacttga aaactatcaa ctggattttt atgtggataa tagctggggc
     3961 tggcttccgt ggaactggtc ggacgggatt ggaaaacagg tcatgtatgg actatatgcc
     4021 attaccaatt ttatttggac aatcagtttg tatgtttcca atgcgacagg ttacttagta
     4081 caggaagcct attccttaga cttcatttcc gctacagcag attccattgg taagaatatg
     4141 cagaccttag ctggtgtgag tgcaaacgga ttttcaacag agggtttcta tgttggattc
     4201 ctcttactct tgattttggt tcttggggtt tatgttgcct atacgggact gataaagaga
     4261 gaaaccacaa aggcaattca tgccattatg aattttgtgc tggtgtttat cctatcggct
     4321 tcctttattg cctacgctcc cgactacatt aaaaaaatca atgacttttc atcagacatc
     4381 agtaatgcca gtttatcact tggcacgaag attgtcatgc cccattccga tagtcaaggc
     4441 aaggacagcg tggacttaat cagagatagc ctgttttcca tacaggttca gcaaccgtgg
     4501 ctactgcttc aatacaacag ttcagacatt gaaagtatcg gtattgaccg tgtggaaagc
     4561 ctgctctcca ccagcccaga ttccaacaat ggcgaagaca gagaaaaaat tgttgcggaa
     4621 gaaattgaag acagaagcaa taccaatcta accattacaa agaccattaa ccgtttaggt
     4681 acagtcttct tcctatttgt cttcaatatt gggatttcca tatttgtatt cctattaaca
     4741 ggaatcatga ttttctcgca ggtacttttt atcatctatg ctatgtttct gcctgtgagc
     4801 tttattttaa gcatgattcc atcatttgat ggtatgtcaa aacgagccat aacaaagctc
     4861 tttaatacca ttttgacacg agctggaatc acattgatta ttacgacagc atttagtatt
     4921 tcaaccatgc tctatacctt atcggctggt tatccgttct ttttgattgc ttttctacag
     4981 attgtgacct ttgcaggaat ctacttcaag ctgggcgatt taatgagtat gttttctcta
     5041 cagagtaacg attctcaaag tgtgggaagt cgtgtgatga gaaaacctcg tatgcttatg
     5101 cacgctcaca tgcaccgtct acagcggaaa cttggacgtt ccatgactac tctaggggct
     5161 gggtctgcca ttgttacagg taaaaaagga cagtcgggtt cggggagttc tgcaaggaca
     5221 caagcagatc actcccgacc agacggaaag gaaaaatcaa cacttggaaa acgtatcggt
     5281 caaaccatcg gtacagtagc tgataccaaa gacagaatgg tagacactgc tagtggtttg
     5341 aaagaacagg ttaaagattt gccgaccaat gcaagatatg cagtatatca aggaaaatcc
     5401 aaagtaaaag agaatgtccg tgatttaacc agtagtattt ctcaaaccaa agcggacaga
     5461 gccagtggac gcaaggaaca gcaggaacaa aggcgaaaaa ccattgcgaa gcgtcgctct
     5521 gaaatggaac aggtcaaaca gaaaaaacag cctgcttctt ctgttcatga aagaccgact
     5581 acaagacaag aacaatatca tgatgaacag acctcaaaac agtctaatat tcagacttca
     5641 tataaggaat ctcaacaagc caaacaagag cgtccagcag ttaagtccga tttttcaagt
     5701 ccaaaagtgg aacgccaagg caataccgtt caagaaaaaa ccgttcaaaa gccagcaact
     5761 tcaaccacta cagcagatag aacttcacaa cgtccaatca caaaagaacg tccgtctact
     5821 gttcaaagag taccactaca aaatacaaga agtagaccac caatcaaaac cgccaccatt
     5881 aagaaagtcg gtaagaaacc atgaagttga aaactttagt gattggtggt tctggattat
     5941 tcttgatggt cttctcactg cttctgtttg ttgccatttt attttcagat gaacaggaca
     6001 gcggaatttc caatattcat tatggaggtg tgaatgtttc cgcagaagtg ctggctcata
     6061 agcctatggt agaaaaatat gccaaagaat atggcgttga agaatatgtc aacatacttc
     6121 ttgcgattat acaggtggaa tcgggcggta ctgcggaaga tgttatgcag tcctcggaat
     6181 ccctcggtct tccacctaat tcattgagta cagaagaatc cattaagcaa ggtgtgaagt
     6241 atttcagtga attattagcc agtagcgaaa ggctcagtgt agatttagaa tcggttatcc
     6301 agtcctacaa ttatggtggt ggtttcttag ggtatgtggc taatcgtgga aataaatata
     6361 cctttgaact ggctcaaagt ttctcaaaag agtattcagg tggcgaaaaa gtgtcttacc
     6421 ccaatcccat agccatacct atcaatgggg gctggcgata caactatggc aatatgtttt
     6481 atgtgcaact ggtaacgcag tatcttgtca caacagagtt tgatgatgat acggtacaag
     6541 ccatcatgga cgaagcactg aaatatgagg gctggcgata cgtttacggt ggagcttccc
     6601 cgactacttc ttttgattgt agcggactga cacaatggac gtatggaaaa gctggaatta
     6661 acttaccacg aaccgcacaa cagcaatatg atgtgaccca gcatatccca ctatcggaag
     6721 cacaagctgg cgatttggtt ttctttcatt ctacctataa cgctggctct tatattactc
     6781 atgttgggat ataccttggc aataaccgta tgtttcatgc aggcgaccca atcggttatg
     6841 ccgacttaac aagcccctac tggcaacagc atttagtggg agcaggacga atcaaacaat
     6901 gagaaaggaa gatttaatga tgaaatttag aaaaaatcag aataaagaaa aacagatacc
     6961 aaaggaaaag aaacctcgtg tctataaggt caatcctcat aaaaaggttg tgattgcctt
     7021 gtgggtactt ttagggctta gtttcagctt tgcgatattc aagcacttta cagctataga
     7081 tactcatact attcacgaaa caactatcat agaaaaggaa tacgttgata ctcatcatgt
     7141 agaaaatttt gtagagaact ttgcgaaagt ctactattca tgggagcaat ccgataagtc
     7201 cattgataat cgaatggaaa gtctaaaagg ctatctgaca gatgaacttc aagctctcaa
     7261 tgttgataca gtacgcaaag atattcctgt atcgtcttct gtaagaggat ttcagatatg
     7321 gacggtagag ccaactggcg acaatgagtt taatgtaacc tacagtgtag accagctcat
     7381 tacagaggga gaaaatacaa agaccgtcca ctctgcttat atagtgagtg tctatgtaga
     7441 tggttctgga aatatggtac tggttaagaa tccgaccatt accaacatac ctaagaaatc
     7501 aagttataaa ccaaaagcca ttgaaagtga ggggacggtt gattccatta caaccaatga
     7561 aatcaatgag tttttaacga cgttcttcaa gctctatcct acagcgacag ccagtgaact
     7621 ttcctactat gtgaatgacg ggatattaaa accaatcgga aaagagtaca tctttcaaga
     7681 actggtaaat cctattcaca atcgtaagga taatcaagtc acggtatcgc tgacagtgga
     7741 gtatatcgac cagcagacca aagcaacgca ggtatctcaa tttgatttgg tacttgaaaa
     7801 gaacgggagt aattggaaga ttgtaaaata acaaatattg gtacatgatt acagatactt
     7861 tgtaatcatg tactcttttt gataaaaaat tggagattcc tttacaaata tgctcttacg
     7921 tgctattatt taagtgacta tttaaaagga gttaataaat atgcggcaag gtattcttaa
     7981 ataaactgtc aatttgatag cgggaacaaa taattagatg tcctttttta ggagctaact
     8041 atatataaat caagtgattt ttgataatta gctgaaatat tctttagatc attgaaaagt
     8101 gaattttgtg tacactgaaa actactgctt tatgcagtac tttaaaaata ttcaattgaa
     8161 tgcttttaaa taag
//
LOCUS       DAEOSP010000164         8020 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.168, whole genome shotgun sequence.
ACCESSION   DAEOSP010000164 DAEOSP010000000
VERSION     DAEOSP010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8020
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.168"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            11..1807
                     /locus_tag="KRH54_002857"
     CDS             11..1807
                     /locus_tag="KRH54_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBG8580495.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGLAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENLQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVISHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(1947..3728)
                     /locus_tag="KRH54_002858"
     CDS             complement(1947..3728)
                     /locus_tag="KRH54_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBG8580496.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(3751..5637)
                     /locus_tag="KRH54_002859"
     CDS             complement(3751..5637)
                     /locus_tag="KRH54_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBG8580497.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(5654..6151)
                     /locus_tag="KRH54_002860"
     CDS             complement(5654..6151)
                     /locus_tag="KRH54_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBG8580498.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(6469..7950)
                     /gene="cls"
                     /locus_tag="KRH54_002861"
     CDS             complement(6469..7950)
                     /gene="cls"
                     /locus_tag="KRH54_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBG8580499.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT         2775 a         1348 c         1137 g         2760 t
ORIGIN      
        1 ggaatttttt atgggaatag aatattttag taaactttta gaaagtagta ctgaaaaaga
       61 attgctctta agtaaaaagt caaataatat aagtacagca agattaatat cttttttgat
      121 agtgatagca gggcttgcta ttggatttta caataaaaat atagtaggta tttttgttgg
      181 agttttatca ataataatat ttatagcact cttagttatt catagtaaag taaaagaaga
      241 agaaacatat tttaaaagca agagcgaagt attaaataag tatattaaga gatttggaga
      301 tggttggaag gaatttaaaa tagatggaaa agaatactta aaagatgaaa acttgcaagc
      361 aaaagattta gacttatttg gaagagcatc attgtatcaa tatatctgtg tagctaatac
      421 atcttatggg aaaaattttt tagccaaata tttgtggaat gaaaatccag atgagaatgt
      481 aattttagaa agacagagag ctataaaaga acttttatca aaagatgatt tttctattca
      541 tattcaaaca ctcagtaata taattggtaa agagcaaaaa aataattcag atagtagtat
      601 agaatcgttt atagagtatg gagaaaataa gaatgtgtat ataccaaaat ggatgcatat
      661 ttttacttgg ggtttaccaa ctgcaacaat tttatctttt atattttgta tgttaggatt
      721 tttacctgtt ttacctgttt ttttactttt tataatccag ttagggttta gtggttttgg
      781 atatccaaga cttatgcaaa ctcttgctcc attattttca tttagtagaa gtatacaagt
      841 atatgaaaaa atgtttgagg ttttagaaaa agagacgttt gagagtgcat atttgaaaga
      901 attacaagat aaactttcta agggaagtgg agtttcaaga ggtgttaaac aattaaattc
      961 aataggaaat gctgtaaacc taaggtataa tcagatacta tatataattg cctgtggtgt
     1021 tttgatgtgg aactatcatt gtgcagaagc tttggagcga tggaaaggta tatatggaaa
     1081 tcaaattaga gattggtttg aatctattgg agaatttgaa gctctaataa gtttaacagt
     1141 tatttctcat gtaaaggaaa atacatgttt tccagtaata aaatatgaag atactccaag
     1201 attgaaagtt gaagaggtat accacccatt aatagcagaa aaaagtgtca tagcaaattc
     1261 gatacaatta aattctcaga cttgtattat cacaggttca aatatgtctg gaaagacaac
     1321 atttttaaga agtatcggtg taaacttagt gctggcatat gcaggtgctc cagtatgcgc
     1381 taagagtttt gatgcgacct gtatggctat atttacttct atgagaatac aagatgatgt
     1441 aagtcaagga atatcaactt tctatgctga gattcttaga ataaaatcaa tgattcaata
     1501 tagtttaaaa gaactgccaa tgttggttct agtagatgaa atttttaagg gaactaactc
     1561 tgctgataga attattggag caagtgaggc agtaaagaaa ttatcaaaac cctgggttat
     1621 aagtatggtg acaacacatg actttgagct atgtgacctt tcaggtagtg gagatgttga
     1681 agttgtaaac tatcatttct cagagtacta tgtagacgat aaaattcatt ttgactataa
     1741 gataaaagat ggaagatgta aaactacaaa tgcaaaacaa cttatgagaa tggcaggtat
     1801 tctttaatta atagataggt taattaaaga ataagtctat ttagaaatat aatttaattt
     1861 gtgaaattaa taaggattgt cttaaattac gtaaaagtat tttaagacaa tcctttttta
     1921 agaataaatt ttatattttg ttatgactac ttagcttctt cacaagcaca ttcttgtgga
     1981 cactctccat agacacaact cttatcaaag taatgagtat gaaggtattt atgggctaca
     2041 tcactatttg gttctccaaa gaagtcttta taaatagctt gtaaatcagg attctcatga
     2101 gatttacgaa tagccttatt tctatctatt tcatataacg catttgcacg tctatcaact
     2161 atttcagtat taccatggtg ataaggttga ccagcacctc caacacatcc accaggacat
     2221 gccataactt ctatgatatg gtatttacat tctccacttc tgaccttatc cataatcttt
     2281 ctagcgtttt taagtgtatt tacaatacac acattaactg tagttccatc aacatctata
     2341 cttgcttccc taattccttt aagacctcta acattagtga agtttacatt atctaaagtc
     2401 ttattagtta tcttctcata tgaagtacgt agggcagctt ctaaaactcc accacttgct
     2461 ccaaatatag atgcagcacc tgtagatttt cctagaggat tatcaaaatc ttggtcttga
     2521 agattaggta aatcgattgc agcttctttt atcatcttag ctaactctct agtagttata
     2581 gaaaggtcaa catctagtat tccagattca cttaattctt ctcttgaagc ttcatatttt
     2641 ttagctacac aaggcattac agatacaaca taaagttctt caggattgat tcctaatatt
     2701 ttaggtgcat agtagttttt agctatagaa ccaaacatac cttgaggaga cttacagcta
     2761 gatgctaagt ttaacttgtc tggataattg tgctctaaga agtttaccca tgcaggacaa
     2821 caacttgtta atataggaag attttcacct ttttggattc tttctataaa ttcagtagct
     2881 tcttccatta tagtaaaatc tgcaccaaag tttgtatcaa atacatgttc aaatccaagt
     2941 gcttttaaag cagctaccat tttccctgta gagattgacc ctggttctaa tccaaactct
     3001 tcaccaatag caactcttac agcaggagca acttgcacta ctatagtctt ttctttttta
     3061 tttaaaacat cccaaagttt aggtacatta tctacttctg ttaatgctcc agttggacag
     3121 acagatatac attgaccaca gaatgtacac tctgtttcaa ccatatcaga gttaaagaat
     3181 gtactaacta aagtattaaa tccacgatta actccagata atgctcctac tgtttgtatg
     3241 tcattacata cagtctcaca tcttctacat aatatacact tagaatggtc tttaactaaa
     3301 gatttagtag aagtatcttt acctgcaaaa gatttagaac cttgatatct tatttttctt
     3361 actcctaaat cagcagctat agtctgtaat tcacaatctc catttttttc acagacaaaa
     3421 caatcttgtg gatggtctga taacaataat tcaacaatag ttctacgagc atttaatgct
     3481 ttagcactat tagtctgaac tttcatacct tcctttataa ctgttccaca agctggaact
     3541 aatcctctct ctgtctcaac catacaaaca cgacaagaag cacaagtgtc aagcttatct
     3601 atttcattca tatgtaaatg acaaaggttg tgtattttta tatttattaa tttcgcagca
     3661 tctaagatag aagttccact aggtgctgaa acgtgtttgc cattaatagt taaattaact
     3721 aaactcattg ttattcctcc tccaaataag ttatttcttg attatagcgt taaatgtaca
     3781 attatctata caagcaccac attttagaca cttagaagta tctattgtat gttgttcctt
     3841 aacagaacct gttatacaac cagcaggaca cactctagaa cattttgtac aacccttaca
     3901 gtcatctgtt ataaagtaag aaagtaagtt ctgacattta ccagcaggac atttcttatc
     3961 tacaacatga gctatatact catctttaaa atattgaaga gtacttaaaa cagggtttgg
     4021 agcagcttta ccaagaccac ataatgaagc agtttgtata gtttcagcaa ggttttctag
     4081 gtcatctaaa tcttgtagag taccattacc ttctgtaatt ttagttaata tttctaataa
     4141 tcttgtagta cctatacgac aaggtgtaca tttaccacaa gattcttcaa cagtaaactc
     4201 aaggaagaac ttggctatat ctaccataca gtcagattca tcaagaacaa gcataccacc
     4261 agagcccatc attgaaccga tagaacttaa tgaaccaaag tctataggag tgtctaaatg
     4321 ttcagttggt atacatccac cagatggtcc acctgtttga acagccttaa atttcttacc
     4381 ttcagggatt cccccaccaa tttcaaatac aatatctctt aaagtagtac ccattggaac
     4441 ttcaacaagt cctacatttt ctacttttcc accaagagca aagactttag taccactaga
     4501 atcttcagta cctaagttgg caaaccaatc tccaccctta agtataattg caggtatgtt
     4561 agcaaaagtc tcaacgttat ttaagcaagt aggagatttc catagaccac tcttagaaga
     4621 actataagtt ttcattctag gttcaccacg tctaccttca attgaatgca ttagcgctgt
     4681 accttcacca caaacgaaag cacctgcacc atattttaat tctaatttaa agttaaatcc
     4741 agtgcctaag atattttcgc caagtaaacc gtatttttca gcttgagcta tggctacttt
     4801 taatctttct atagattttg gatattctgc tcttatgtat atgtatcctg tatcagaacc
     4861 tatagcgtat ccacatattg ccatagcttc tagtacacta tgagggtcac cttcaagtac
     4921 agatctatcc ataaatgcac ctgggtcacc ttcatcagca ttacatacaa caaacttttt
     4981 atctgttgga ccagctggat tttttgaagc agcttcccat ttagagccag tagggaaacc
     5041 tgcaccacct ctaccacgaa ggccagaagt ttttacctct gcaataactt cttgaggagt
     5101 aagagaagtt aaacatttgc ctagagctaa ataaccatca ttggcaatat attcttctat
     5161 actatctggg tctataagac cacaattttt taaagctata cgtaactgtt ttttgtagaa
     5221 agacatttct tcttgttctt caactttttt acttaaagat tcttcctcat ataataattc
     5281 ctcgacaact gtatttctaa ttaagtggct ttgaactatc ttttcagcat cagatggctc
     5341 aacttttacg tagaaaacgt tatcaggata aacttttaca ataggacctt gagcacagaa
     5401 accaaaacaa cctgtcaaac gaacactaac tttatcttgg attcctgatt tctttatttc
     5461 attttctaat tcactaacta tttctagact gtttgaagat gtacatccag tatcacaaca
     5521 aacaagtaat tctcttctta atacattttc cttagaataa ttttttctta agctaagatt
     5581 aggtttcagc tcatcagcta aaacttttaa ttcatcaaat gaatttactt tacgcattat
     5641 ttattcaccc ctcttaacaa ttcaattcta attctctata agtatctaat atttcgctta
     5701 cttggtcagg tttcacttta ccataaactt taccatttac agtaacaact ggagctaaac
     5761 cacatgcccc caaacatctt aaaccttcta aagaaaattt aaaatcacta gtagtttctc
     5821 caacttttat gccaagctgt ttttcaaaag cacttaagat tttgtcagca ccttttacaa
     5881 aacatacagt tcctagacag acactgattt tatacttacc tacaggttct gtagtaaaat
     5941 aagaatagaa tgtaacaacc ccatatacct ttgcaggagc tactccaagt tttctagcaa
     6001 catgaagttg aacttctttt ggaagatatc caaatatacc ttgggcttca tgtagaactt
     6061 gtattaacgc accttccttt gttgcaagag agtcaataaa tatatctagt tcgtcaaata
     6121 attgcttgtt gtgtgaaata aaatcgcaca taacaaatcc cccttttctt tttaaagtta
     6181 ttatttttca taaaataatt aaccatgagt tgaccccaac attatgtggg tgaacaaaac
     6241 caaaacaata aagaaagaat aatatacaac aatttcttta tttataaaaa caatgcaaaa
     6301 aagttaccta ttaaaaataa aaactatact agtctattat aatataaaat aattcagaat
     6361 agttaaaaaa aataaaaaaa atgagctaaa ttcaataaaa aaaaaataat acatataaaa
     6421 aactttaaaa aataaaccaa aatgtaaaat ttttataaaa taaaagattt ataaaactgc
     6481 tgataatagt cttgaaattg attccttgat ttttatataa gtagacctag aattatatat
     6541 atctaatgtt atttccttag aatttaaaat atcattttca aatataattc tttgttccaa
     6601 tgcttttttt gaagagtaca taaatgcatt aacttcgaaa ttaagctcaa aacttctgat
     6661 atccatatta gctgttccta tagagcatat agagtcatct ataactattg tcttagcatg
     6721 taaaaatgca ttttgaccat aagtatagat tttagctcca aactttaaaa gttctccagc
     6781 atatgaatat gatgcccagt atacaaaggg atggtcaggt ttagaaggaa tcatcactct
     6841 cacatcaaca ccagataaac aagctatttt taaagtatct ataaaagttg agtcaagtat
     6901 taaataagga ctctgtatgt atatatactt tctagctttc tgaatcattt taaggtaacc
     6961 atatttaatc tcatcaagtt ctgatatatc tggaccactt gatactatct gaatcccaat
     7021 attttcagaa ggatgattag aatttgtatc ttcttctata aagtattttt ctaaatctaa
     7081 gtcttcttta gttgtatatc tccaatcaag gatgaatctc atatttaggt caattacgca
     7141 atcaccagta agcttagtat gtgtatctct ccatagacca aattttggat ttctacctaa
     7201 atattcatca ccaacattat atccacctac aaatcctacc ttaccatcaa tgacaacaat
     7261 ttttcggtga tttctatagt ttaagttaaa gtttacaatt ttcaagaatg atgggaagaa
     7321 gcttccaact ttaacaccac tttctttaag tctggaaagt gtactatttt ttagtgttct
     7381 accaccaact gcatcaaata aaagtcttac ttcaacacct tctttagctt tgtctataag
     7441 aatgtctatt atttcagttc cgattttgtc atctttgaaa atgtaaaatt gtatgtttat
     7501 atatttttta gcttttttaa gttcttcaag aagactgtta aaaaattgac tagattcagc
     7561 ataaatccac acatcgttat tattggtata atgagcatta tttgaattag ccaatgcata
     7621 aatcatatcc ttatgttgat gtatatctga atcaatttca gatgtagatt ggagtttaac
     7681 ctgagtatct agtatattac ttttgattat cttatcatcc ttttctttca atctaaacat
     7741 attgtttttt gaaatatttc tgccaaaagc tatataaagt atgaatccaa gagcaggaat
     7801 caatactaat ataagaagcc atgttaaagt tgtatttatg tttctttttt ctctaaagat
     7861 aaggtttagt ataaccatga aattaattat atatattgta gtaactgcta tttcatatat
     7921 tgacatttcg aaaaatgata tatcaaacat aaaattccct ccttcaaata tttattaaaa
     7981 taagtaatta tatataataa cacaaacata tatctattat
//
LOCUS       DAEOSP010000165         7920 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.169, whole genome shotgun sequence.
ACCESSION   DAEOSP010000165 DAEOSP010000000
VERSION     DAEOSP010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7920
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.169"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     misc_binding    137..348
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            511..1800
                     /locus_tag="KRH54_002862"
     CDS             511..1800
                     /locus_tag="KRH54_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBG8580500.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1854..2933
                     /locus_tag="KRH54_002863"
     CDS             1854..2933
                     /locus_tag="KRH54_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBG8580501.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2954..4498
                     /locus_tag="KRH54_002864"
     CDS             2954..4498
                     /locus_tag="KRH54_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBG8580502.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4661..5203)
                     /locus_tag="KRH54_002865"
     CDS             complement(4661..5203)
                     /locus_tag="KRH54_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBG8580503.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5351..5635)
                     /locus_tag="KRH54_002866"
     CDS             complement(5351..5635)
                     /locus_tag="KRH54_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG8580504.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5698..6462)
                     /locus_tag="KRH54_002867"
     CDS             complement(5698..6462)
                     /locus_tag="KRH54_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBG8580505.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(6744..7010)
                     /locus_tag="KRH54_002868"
     CDS             complement(6744..7010)
                     /locus_tag="KRH54_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG8580506.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(7052..7861)
                     /locus_tag="KRH54_002869"
     CDS             complement(7052..7861)
                     /locus_tag="KRH54_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8580507.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT         2768 a         1001 c         1286 g         2865 t
ORIGIN      
        1 atacattata taaatatagt aatagtatga aaatttacat ataaaactaa aaaatgcaag
       61 taattgactt tttaagttga caaaatattg catatatatt atgatataaa tcataaatta
      121 actacaaatc aatattatat attcgtagaa gaagaaagta gcataaagga attttacaga
      181 gagttagatt tggctgagaa ctaatattgg aatttatgtg aaaaggactt tggagaatac
      241 tattgtaatt atgttagaat agtggtttaa ttcgccttaa gaaaatgagt gagtaaaatt
      301 aatttttact aataagagtg gtaacacgga tttatattcg tctcttggat ttttccaaga
      361 ggcgtttttt attataaact tatttcagaa aatttaaaac tatatattaa atgaaaatta
      421 taaaaatatg atagttaata aatttataaa ttagaaaatt gtaaaatatg ctaaggtcag
      481 tagttttacg atttaaatgg agggatgaaa atggaagaag aaaaaattaa ctatgctctg
      541 gaacagattc cagagaatca aaaacgtggt tgggtagcta tgttttcagt tttagtagca
      601 ataggtgtgg atttatcatc tgttatttta ggcgctgagt tggcacagag tatgccaatg
      661 aaacaagcaa tattgtcagt aattgttggt tcattttttt ctgcaatttt gtatactaca
      721 tgctctttgg ttggttcatc tacaagttta tctacatcta tgataacaaa gtatgtattt
      781 ggagaagctg gagcaaaaat attttctcta gttatagggg tttctctatt aggttggttt
      841 ggagttcaag taggtttctt tgctcaaaat gcacagataa taataaaaga tatatttaat
      901 ttagatgtca gtatgcagat tttaagccta ataggtggtc tcttgatgat gagtactgca
      961 atttatggat ataaggcaat ggagaagttg agtgtgtatt cagtaccatt tttattagtt
     1021 cttatgatgc taactatatt cttagctttt agagcaaatg gaatttcagt tgatgataat
     1081 atgaaatcta ctatgacatt tgcaggtgga gtttctctgt caatgagtat aataatagtt
     1141 ggtgcaattg tatctcctga tataagtcgt tgggctaaaa gtagaagaga ctgtgctctt
     1201 tcaagctttt tgggaataca atttggaaat gcttttatga taatagtttc gatagtatta
     1261 gtaaaatgta tgggaacttc agatattatg agaattttta ttacattagg aattgctata
     1321 ccaggtataa tagtacttac attagcacaa tggactacaa atacaagtaa tgtatattca
     1381 gcatcattaa gtatagctct tgtacttaag aaggctcctg agaaagtatt aactatagtc
     1441 cttggtatta tagcaacatt attggctgta tttggaatat atgaaggttt tataggattt
     1501 ttaaatttac ttggaattgt aattgcacct gttggtggtg tatatacagc agaatattac
     1561 atagtaaagc aagaacttaa aggctttgat aaaggagtat tatataaacc aatagttaaa
     1621 cgttcaatag tgtcttggat aattggtata ttaataactt acctttcaac atatggattt
     1681 ataactctta ctactatagc acctttagat ggttttatag cagggtttgt agtacaatct
     1741 attattggaa aagtgctttg ttcaacaaaa aataagcaag aaatagacaa agcagtataa
     1801 agaaatttgt ttataaagta taaaataaat tagaaatatg aggagaaagt aacatgagat
     1861 ttttaacaga agaaagtgta gataaaatag cagttggtgc agcagtgctt ggaactggag
     1921 gtggaggtga cccatatgta ggtaaattgg ttgcaaaaca agccattaaa aagtatggtc
     1981 ctgtaaaagt aataagttta gatgaacttg atgatgatgc tttggtagtt ccagtatctg
     2041 gtatgggttc tcctgtaatt acaatagaaa aattactttc agaagtagag ttaactaccc
     2101 ctttagaaat aatggagaaa ttattaaata gaaaagtgga tgttataatt cctatagaaa
     2161 taggagggat aaactcactt atgcctatag cagttgctgc taaaaaagga cttcctatct
     2221 tagatgcaga ttctatgggt agagctttcc ctgaagctca aatggtaact ttttatcttg
     2281 aaggatatga ggcatctcct gccgtaatgt ctgatgaaaa aggtaattct gccatccttt
     2341 atcctgtgga tggaatttgg tcagaaagat tagctagaac tttaactgta gaaatgggag
     2401 gaagttcttc tattagtgac tataatttaa gtggagctca agttaaaaaa gctgctatag
     2461 gtaatactct tacaatagct gaaactatag gagggttttt acttgaaaat aaatctgatt
     2521 caaaaggtgc tgttgagaat atactaaaag aattgtgtgg atacaagtta tttgaaggaa
     2581 aagtaattga tattaagaga gagttgaaag gtggatttac tagaggacat gccttttttg
     2641 ctgggattaa tgagtatgat ggagagtatt ctatattatt ccaaaatgag aatttaattg
     2701 ccaaaaaagg tgatacacca ctttgtatta caccagattt aatagcagta ttagatttag
     2761 aaactggatt tccaataact acagagagaa taaaatatgg ctctcgtgtt atggtagtag
     2821 cttttccttg taatgaaaaa tggagaacag aaaaaggaat tgagacagtt ggacctggat
     2881 actttggata tgatgtggaa tataaaacag tagaagaatt acaaggaaaa taaaagttta
     2941 ggaggaaatt ttgatgtatc gtgtaggaat tgacgttgga ggaacaaata cagatgcttg
     3001 tatactggat ggggaattaa aagtaattca ttcagttaag gtggcaacta caaaagatgt
     3061 tgaaactggt gtttacaatg ccttaaaaaa agttatagat gaaagtaaga ttgaccattc
     3121 attaataaaa tatgctatgc ttggtacaac tcattgtaca aatgctattg tagaaagaaa
     3181 aagacttaat aatgtggcta tgataagaat aggaaagccg gccactactg ccattcttcc
     3241 atttatagat tggccagaag acttaagaga aaaaatagaa ttagattcaa tattggtatc
     3301 tggtggatat gagtttgatg gcagaaaaat aaatgaactt tgtaaagatg aagttgtaga
     3361 gttttgcaat agaataaaag gcaaagtgga aagtgttgca ataagtggag tatttgctcc
     3421 agtaaataaa caacaagaag aagaagttgc aaaatgggtt agagaaattt taggggatat
     3481 tcctatatct ttatcaaatg aaataggtaa tataggtata ttagaaagag aaaatggagc
     3541 tattttaaat tctgcattgt ctcaagtagg taaagctgta tctttaggat ttgaaaaagc
     3601 acttaaagat ttaggtatag atgctcaaat atatttcagt caaaatgatg gaacattaat
     3661 gaatttagag tatacaatga aatacccaat ttttacaatt ggatgtggaa taacaaattc
     3721 tataagagga gcttccttct tatcaaaagt aaaaaatgca atagtattag atgttggtgg
     3781 aactacaagc gacttgggtg tactatataa tggattccct agagaatcat ctattccagt
     3841 tacaattgga ggagtacata caaactttag aatgccagat gttttaacta ttggtcttgc
     3901 tggtggtaca tgtgtaaaag gcgaaaaaga taatataaag ataggtcctg aaagtgtggg
     3961 atatagaata actgaagatg caataatatt tggtggagac actctaactt tatcagatgt
     4021 tgttacaaaa ttagatatgg cgtttgaaga tagaaaaagc aatgttgaaa acttagataa
     4081 agagtattgt aatgaggtat ataagaaggt tattttaaaa ttagaagatg ctgtagacca
     4141 aatgaagact agtcaagatg atgttgaatt ggttttaact ggtggaggaa gtataattgt
     4201 tcctgaaaaa ctaaaaggca taagcaatgt tataagacct cctcattata ctgctgcaaa
     4261 tgctattggt gcagctttag gacaaataag tggagatgta gaaaaagtat acagtttaga
     4321 taaaatgtcg agagaagatg ccatagaaga tgcaaaaaaa gaggctgtaa ataaagctct
     4381 aaaagctggt gctaaagaag atactataga agtattaaat atagaagatg ttccacttgc
     4441 ataccttcct ggtaatgcac ttcttataaa agtaaaagtt gttggtgatt taatttaaag
     4501 aataaatata agtatttcca gaattatata aatatttctg gactcatttt taaacttatt
     4561 ttatcaataa aaacagcata acttttgact atattcttat aagtttccga acagccgata
     4621 tatacctatt ttttgaggtt tatcggctgt ttgttatctt ttatcgcatt ccaactattt
     4681 ttcttaccgt atttgctgta cgaattgtaa tcttatcact tacatttgaa ctggcagtct
     4741 ttgaccacca atttgtcttt ttataatctt tacgactaaa agaccaaaag atagcatttt
     4801 tataatgata tactttttca tactcttctt taggatttcc aacctcatca taaaactcgt
     4861 cataagataa gtcaggaaac ataaaaataa gattatcata tatttctttg ttgttatctc
     4921 cccaccaatt aggagcatta tttagtatat cttttaactc ttcttgtgaa ataataaaaa
     4981 ctggtatttc caattcaaac tgttttttta tcattaatct tattgtattt gagataacct
     5041 tgctgtcatc tacatcactt aaaaagacaa cattaccact attaaggtat gtagaaactt
     5101 ctgaataacc aagttctaca aagcttgctt ttaattcaga cataattatc ttgttcttac
     5161 cacttatatt gatacctctt aataatgcga tataactttt catataataa tttcacctct
     5221 aaacattcac atttttctat gtagtacaaa cttgaatttg catcttttat aattctttca
     5281 aaacttcttc atatgtaatt tttttaatat aaaaaaatcg gtgcttaatt tgtaccgatt
     5341 tttgtttttc ctagtaaaga actcgttttt ttgcttctgc tatagctcta gcagcacttt
     5401 ttgcttgcat tcttttttct tcttcttttt taaattcatt tattgtttca tttagattct
     5461 tatcatgttc atttttttct ttatcttcgt tttctctgta tactaatata taatctatta
     5521 ttgattcgtt tattatctga gatatacttt tattttcatt aattcctatt ctcttaactt
     5581 ccttaagtag agtctcatca atatgaaccg ttgtttgctt tgtatttttt gccaccttaa
     5641 tcacctctta aaaaaaattc taacattaaa atttttttta gtcaataaat tttaatatta
     5701 aaattcttta aatgttttat ttgattttct aaaattttct attgtattta tcatcttatt
     5761 ttttatattt tcacctagta cagaagactt tagtctagga ttttcgatat ggtcataaat
     5821 tggttctaaa aattcaactt cgacaggaac agagttaatt ttaccaatag gttggtatcc
     5881 ttcataagta tcttttgaat tttttattac gagtggaaca atagggcatt ttgctttata
     5941 tgctatcttt aaagctccac ttctaaactc cgaaaccaat gaatttggtt ctttaatcca
     6001 tgttaaatct ccttctggaa acactgccat actttgacct gtaagtatat tttgagaggc
     6061 ttgggctata ctctttatac cctcacgatt gttttttctg tttacatata cacatcttag
     6121 taattttgcc cattctttaa atataggtat atttctccaa actggttcat ctgcaattac
     6181 acatccaatt ggtctttcta cacttgctgt tagtatgaag ctgtcaagca tactagaatg
     6241 atttacaaca aatagaagtg gttcttttgg cagtgtttcc ttaccaatta tattaagctc
     6301 tatattcact atatctaaag attttttagc ttgtttttgc ataaattcaa atttttcctt
     6361 taagctaaat ttatcaggat tctttttaat tttaataagc ctaggtatag aagatgtaaa
     6421 acctataagt tgatatgtag caaattttaa gtaattattc ataaaattat ctccttaaaa
     6481 taaaattatt tattactgta aagattttaa cataaaataa aatttaagtc tattttaatt
     6541 caattttgta acatatattt taaatagaca gagattgaga ttttatattt ccaattttag
     6601 taatcagatt ttatcaataa attactattc tattaattta attaaataac ctttaaattt
     6661 aattataaaa actgttgcag aaatttctac aacagtttaa aaaatttacc ttacaaaaac
     6721 ttattttata acaagagagt atattataag gctagtacag cccatttttt gcactgagaa
     6781 attcttttgc catcatcacc ttgttctttg tcatatgcaa attgattaag catttcacat
     6841 ggaaatgtat cacatagacc acaatgtgta aggtttttat cttcacaaca agattttaca
     6901 ggacattgtt caccccaaaa tggtttctca atctctgtac aacctttaca attgactttt
     6961 tccttgtatc cacattcata acataaaatt ccacatcttg attctatcat tttaagtcac
     7021 tccttaaata taaattttat atgattgtat atcaaaattt atacttttca ttgtaaaaat
     7081 cggacaaatt ttttatatag gttgttggat taacaccaca aatagattta aaatcattta
     7141 taaaatgaga ttggtcgtaa taaccaatat cttgtgctaa agttgcaaat ggtattttat
     7201 tattttgtat ttcttgtaat acaaggttta tacgaagtaa tctaaggtat gatttaacac
     7261 tcataccaag agaattatta aaaattctat ttaaatgtcg ttcactataa caactaattt
     7321 gtgaaatgtt tttaacagac ataatagagt tttgttttct agcattttca agaattggta
     7381 tagtcatatc tactatattt gatttcaata agtatgataa gaataacata tctaattgtt
     7441 ctactaattc ctttatagta cttgttcttt caaaaatgga attaacctct aagctaaata
     7501 atgtgttaaa atcttcaagt gaaattttta aatctgtaga ttctctttga gaaagacctg
     7561 tcaaataata tactccacct gggcgaaact caacgaatac tctaaaaagt acattttcta
     7621 tatcattttt tacaatagta gtttttgttg tggctcccca gaatgcgctt tctatacctt
     7681 ttttgtcaaa tttaaatatc atacatccac tagcatcagg tattaatgta agattttctt
     7741 taatacttgt atctggaata gatattgtat agtgtgctat atactgtctt aacaatttat
     7801 gtggcaaaat atatgtatac tgtttgcagg tatttaaaat gttattgatt attttattca
     7861 taagtatatc ctcctgatat ataaaaaata agtatactaa tttatttttt atataataaa
//
LOCUS       DAEOSP010000166         7874 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.170, whole genome shotgun sequence.
ACCESSION   DAEOSP010000166 DAEOSP010000000
VERSION     DAEOSP010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7874
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.170"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            478..1884
                     /locus_tag="KRH54_002870"
     CDS             478..1884
                     /locus_tag="KRH54_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG8580508.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            1912..2760
                     /locus_tag="KRH54_002871"
     CDS             1912..2760
                     /locus_tag="KRH54_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG8580509.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            3421..4113
                     /locus_tag="KRH54_002872"
     CDS             3421..4113
                     /locus_tag="KRH54_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8580510.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLAELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCAKHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            4110..5045
                     /locus_tag="KRH54_002873"
     CDS             4110..5045
                     /locus_tag="KRH54_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8580511.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTIFTVSLPLI"
     gene            5193..6116
                     /locus_tag="KRH54_002874"
     CDS             5193..6116
                     /locus_tag="KRH54_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580512.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            6100..6870
                     /locus_tag="KRH54_002875"
     CDS             6100..6870
                     /locus_tag="KRH54_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8580513.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            6880..7662
                     /locus_tag="KRH54_002876"
     CDS             6880..7662
                     /locus_tag="KRH54_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8580514.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
BASE COUNT         2732 a         1060 c         1489 g         2593 t
ORIGIN      
        1 tgaagcagga accctgtgac tttagtcatg ggaggttcag tttgggcaag tagtttcttg
       61 taaatctagt tatgtttcaa ccaatgataa tataatttca ttagtattag ctggtaatta
      121 agttaatttg actagagata agtaaattga tttaaaagga aataagtaaa tcaacaatta
      181 aatacatcta aaagtaagtt tattgcaatg aattgttaaa gaaaataatt aatatggagt
      241 aaatctattg caatgaagta acaaagaaaa taattaatat agagtaagtt tattgtaatg
      301 aattgttaaa gaaaagagtt aatatagagt aagtttattg taatgaattg ttaaagaaaa
      361 taattaatat agggtaaatc tatctcaatg aaataacaaa gaaaagagtt aatatagagt
      421 gaacttattg caatgaatca acaaagaaaa taatcaatat aaaaaggaga aatatttatg
      481 aatacaggat ttccaaaaga ctttttatgg ggagcatcat cttctgcatt tcaagtggaa
      541 ggagcttggg ataaagacaa taaaggaaaa accgtagccg actataattc ttttaaaaag
      601 tctcatttac aggcagatac taaagtcgca tcagactttt accataatta tgaagaagat
      661 attgaattaa tgaaagaatt agggatgaaa acatatcgtt tttcaatttc atgggcaaga
      721 ataataccag atggtgaagg agagataaac cagaaaggtt tagactttta taataagata
      781 atagacaaac taatagagtg tgatattgag ccattcgtaa ctttatacca ttttgacctt
      841 ccatttaaat tagtagagaa atataatggt tgggaaagta gagaaactgt ctatgctttt
      901 gaaagatttg ctaaaatatg ctttaagcat tttggagata gggtaaagta ttggcaacct
      961 cataatgaac agaatttaat tgttagagtg gaggagagaa ttaatatata tgatgaaaca
     1021 gattcatgga agatagataa aatgcgtgct cagatggatt ataatctttg cttagcacat
     1081 gcattagcag tcaatgcatg tcatgagatg ataaaagaaa gtaaaattgg agcagcagta
     1141 tcatcatctg taacatatcc tcttacttct aagccagaag atgtatatgc agctagaatg
     1201 aatgataatt ttaaagtgta ctatatgctt gatatgcatc attatggaga atatccaggg
     1261 tattatatga agtatcttga aaaaagaaat atagttcctc acatggaaga tggggataaa
     1321 gagatactta aaaaagctaa aatggatttt atagctgtaa attattatag gacaaattgt
     1381 gcagaagctt tacctgaaga ttcacaacat ccatttggat taagagaagg aactgttgat
     1441 tttagtatgt atggactatt taaaatgtca atgaatccta acttagaggc ctctgagtat
     1501 ggagcagcaa tagacccatc aggtcttaga gttgccttaa atgaatattg gcaaagatat
     1561 cacttacctg ttataataac agaaaatggt ctaggagcta aagatatatt agaagatgga
     1621 aaaattcatg atgactatcg tatagactat ttaagaagtc atataaatgc atgtaaacta
     1681 gctattgaag atggtgtaga gatgattgga tattgtccat ggtcatttac agacctttta
     1741 agttcttctc aaggttttaa taagcgttat ggacttgttt atataaatag aactgaccat
     1801 gaagttttag atttaaaaag aatcaagaaa gatagttttt actggtataa agaagttata
     1861 gagaacaatg gaatagtaaa ataaacataa atatatattg agggttttag tatgtacatt
     1921 acaaaggtat taaataattc gttgctactt gcaaaggatg ataatgatga agaaataata
     1981 ttgatgggga aaggcattgg ccataattat aaggccgggt ctgagttaaa taaaaaagat
     2041 atagaaaaga tttttgttct tcatgatgaa aatataaaaa agagcattat acaattggca
     2101 acagaaatag atgcagaata ttttggtata gctcaaatga taattgcata tggaatagaa
     2161 aaatataact taaaacttat gaaccatata tatttggcct taactgacca cattgccttt
     2221 gcagtgagac gttttaaaga aggtataatg attgaaaatc actatttgtt cgaaatcaag
     2281 gaatttaatc caaaagagta tgatatagga aaatatgcta ttactgtttt taaggaggtt
     2341 cttggtttag acctaccaga agaagagatt ggtaatatag caggccattt tataaatgca
     2401 caacaagata atccttatag tgatagaaat aagagaagtg ctaaaattgt aaatgctatt
     2461 ttgcaaatag ttcattatca cttttctatt gtatataata aagagtcatt ttattatagg
     2521 agatttgtaa tgcatctaaa ggccttttca caaagatttc tggctaatga accatcaaga
     2581 gaaaaaatag attttatata tgagcaagtt caaaaaaatt gtaaggaaga gtatgaatgt
     2641 gtgaaaaagg taggagctta tatgtataag gaatactcca gagaattacc aagacaagag
     2701 gagttatatt tgatgattca tattcataag attttaggag aattatgcga aaatagttag
     2761 aatatcttaa tatgctagta atattataaa aaattaaaaa ttaactacac tttaaatatg
     2821 aataattgca ttacttaagg gggttgtctt aaaattgatt tttttaatta aatttgaagc
     2881 tcaactcctt ttttaaacct ttatatcatt acaactttaa tgtttatacg cctgtacttc
     2941 agatgtatat ctacttttta catttctcaa catgcaaatc taccattttt tgcagtatgt
     3001 taattgaaat aaaccattga gttaacccta aatttattct ggacttatag gaatagttcc
     3061 aaagatactt taaaatattt tctggaataa ttgcttattt cacatatgtt ttattgtgat
     3121 gttttgattt aataaattct tcaactctgt atatagtaaa tgtattatca tgtttgtttt
     3181 attcaagcac acttataata cgcttgattt tttcacaatg atgaaatata ctaatgggtg
     3241 ttataagtat aagaatagca ttacacttga agaagcaagt gataaatgag gtataaatcc
     3301 tcatatgata aactactatt tcattgagga gcatattcta aatgatgtaa agatactaca
     3361 atatgactaa attcctaatg ataaaaaagt ctacgaatga gtgtagaaag gggaactaag
     3421 atgaataaag ttttaattat agatgatgat aaagatcttt gtattttgtt aaaaaagagt
     3481 atctcaattg aacagattga agcaaactat tgccattctg ggagagatgg actggctgaa
     3541 ctttcaaaac aagattatca gttagtagtt ctagacgtga tgatgccagg tatggatggt
     3601 tttgaaacta tggaacaaat acgaaaatat agcagtattc ccatcttaat gctgacatca
     3661 aaaagcgata attacagcaa agtgcatgga ttgcgagctg gtgcagatga ctatctaacc
     3721 aaaccatttg aaattacaga atttactgct cgtgtactgt ctttaatccg acgttacacc
     3781 cgttttaatc caaaaaatga ggagccacag caactttctt tcaaaggttt gttgattgac
     3841 cttgaccagc atacagtgac taccaatggc gaacctatgt tgcttccacc caaggaattt
     3901 ggtgtgcttc tttactgtgc aaagcatcag ggtaagattt tgacaaaaca gcagatttat
     3961 gaagccgtgt ggggtgagcc atatgtatat gatgacagca atatcatggc tattatcagt
     4021 cgcctaagaa aaaaaattga actagaccca tcatctccca tctatttgca aacaataaaa
     4081 ggaattgggt atcaattcaa cacggaggta tgaaaatgga cgaacaaacg ataattcttt
     4141 tcttactctt gctgtcgatt ggtctacttt ttctttccat atttgctttt tccaagcttg
     4201 ggcatattta caagcgccta aaagatatag aggagatact ggctgatgta caaattggag
     4261 acgaaaatcg taaaatacta attaagccat gtgatgttat ggctccattg gtttatcagc
     4321 ttaatgagat tgtctacgat tatgaaaata agttactttc tctgaaaaag tcggataaag
     4381 ctagcaaaca acttatgaca agtttatctc atgatgttcg tacaccatta acaactctga
     4441 ttggttatct ggatgctgta cacagtggta tagttatcga acaagaacga gaggaatacc
     4501 ttgaaatcgc taggcgtaga gcctacgacc tcaaggatta cattgatgtt ctttttgact
     4561 ggtttcggtt gaactctgat gaatttacgc tgtcaataga atctgtagaa attgcagagt
     4621 tgtcacgcaa tattttgaaa gactggattc ctatattcca tgaaaagaaa ctgaattttg
     4681 aaattgatat tccagaaaat cgactaatgg taaacctcga cccagatgga tattcccgtg
     4741 tggtcaataa tctggttcaa aatgttcttg ctcacagtaa ggctcggcaa atcaagatta
     4801 ccatgagtga ggattccaga atggtattac tccgtgttga ggatgatggt gttgggattg
     4861 ccagagaaaa tttaccacat atttttgagc gactgtataa gtgtgataaa ggacgttcag
     4921 aaaaaggaag tggtcttggt ctatctattg tcagtcaaat ggtggagcga atgggtggtc
     4981 agatttccgt agaaagtgag attggaaagt ataccatatt tacagtgagc ttaccattaa
     5041 tataaaattg tttttccccc atgtgacatg tatacacagg gggatttttt gtaagttata
     5101 aagttgcaag gttaatacaa ggttgttgca aggttaatgt aagattgagg tggtatgata
     5161 actttaataa acaaaggagg cttttattat ttatgaataa tttaattatt gaaacaaaaa
     5221 atctcactaa gcaatatggt gctcaaaaga gcgtttctaa tttgaatatt cacgttcaga
     5281 aaggtcgcat ttacggattg ctaggaagaa acggtgcggg taagacgaca actatgaaaa
     5341 tgttattggg tctaacaaaa ccaactactg gagaaattaa aatatttggt caggacattc
     5401 acaataacga gaagaagctt cttcctcgaa ttggatgttt gattgaatca ccagggtttt
     5461 atcctaatct gacaggaaca gaaaatctca aaatctttgc aaggcttaga ggaataccaa
     5521 aaaaaaatgc agtagaatca gcactggatg tagtcggttt gccaaacaat gacaaaaagt
     5581 tattttctca atactctctt ggtatgaaac agaggttggc gattgctctt gccattatgc
     5641 atgacccaca actgttgatt ttggacgagc ctatcaatgg gcttgatcct attggtattg
     5701 cagaagtaag aacatttatt cgtgatctct gcacagagcg aggtaaaact attttgattt
     5761 ctagtcacat tctttctgaa attgccttgt tggcagatga cattggcatt attgaccatg
     5821 gtattttgtt ggaggaagaa agtcttgccc aattagaaga aaaaaacagt aaatacatac
     5881 attttgctgt atctgatgca gcacaggcag caaggcttct ggaaacccaa cttaacctga
     5941 aaaatttcaa agtagcagat aattacaata ttatgctata tgatacagat gtttctgttg
     6001 cagaaataaa tcgtgctttc attctaaaca acattgaggt atcagaatct catttatgcg
     6061 aagatactct tgaagattac ttcaagaaag taacaggggg tgaaggaatt gcttgatata
     6121 atttcatgtg aattttcaaa actcaagcgg ttaaaattta tactgatttc tattttaggt
     6181 gcttgcttat ttccaattcc cgcaacaatt ttgattgcaa aggataactt gccctttgag
     6241 caattgttca aattagttgt caattttgga tattttttgc tattaccaat tgtattaagt
     6301 attgttgcta gccaactatt ttttatagaa agagataatg atgtattaaa aaatcttgca
     6361 acagtgccag tgcaaaaagg aaaactggct ctggcaaaat tggctgtgtt gctgtttata
     6421 tcattgttct actctgttgc agggttagga gctactatca ttggaggttt cattgttgga
     6481 atagtagagg gggttgctgt aaaattagga atgagcattg cttttggaat tatgtttttt
     6541 gttactgtac ttcctgttgt ggtactcatt gtatatttca atcgcagcta tattttttct
     6601 attatacttt cttttgtata tgctattttc aattttagta tatccttaaa tattatcaat
     6661 tttgaaccaa ataacccaat attaagtgta ttacctgctc ctgttataat gcgttggtgg
     6721 atggcatttt ggggagatcc cactggcgaa tacacggcac ttagacagcc atatttgctc
     6781 tcaactcctg cttgtgtagg tattttatgt ttgataacca ttatagcagt cctgctgatt
     6841 tgtatgatat acaaaaagca ggagaattag gaggtatcta tgcttgatat aattactaca
     6901 gaatttcaaa aaataaaaag atataatatt ctatggattg gtgttgttgc tgttctgttt
     6961 tcagcactat tggcagtttt tcaggttgta agttcacacg gcagtgaccc tctaacgtat
     7021 gagagctttg caaacggagt tatctggaat aatttcagtc ttagttttcc atttgggatt
     7081 acacttattg gaggatatct tattaaccgt gaatatacag accaaacctt aaaaaacatc
     7141 ctaactgttc ctatttcatt acgcaaattg ctagtgggaa aattaattgc ggttggagga
     7201 attactgtat tgttaagtct tttcagcttc ttatgcacat ttattttggg gttgttattt
     7261 tgcaatatta atatatctgt tacactcgtt gtaaaatcct tagttcagat tttgtctgtt
     7321 aatctgtact gttatgttgc tgttttgccg attattgctt attttggtcg taaacaaaat
     7381 gcatttctga cgggtgtagg gattgcattt gtatacggtt tttgtggtgt gtttgttgct
     7441 ggaagaaatc tgacagactt ttatccaatc acagcaggtc ttggtctagt tggatacaac
     7501 aatggtgctg gtactgttta tcagccactt attggtgcta tgactttgat tgtaattcta
     7561 atcttgacaa ctattctact tgtatttaca cctaattatg ataaagttat gaccatatct
     7621 aagaaaaaag ggataaagca taaaaagtat tctcataaat aacataaata ttgtttctat
     7681 tatttaagtt gctagacagt gaaaagaaaa aatctgtcgt acagtggcta tatgctcatg
     7741 tattcaagaa gctacagttt tgatttttca taactgtagc ttctttttgt atttattaaa
     7801 tcacgactat cataatactt gcaataggta tagcgacaag agagagtact gtcatatgtg
     7861 ttttatatat taca
//
LOCUS       DAEOSP010000167         7795 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.171, whole genome shotgun sequence.
ACCESSION   DAEOSP010000167 DAEOSP010000000
VERSION     DAEOSP010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7795
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.171"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..1332)
                     /locus_tag="KRH54_002877"
     CDS             complement(<1..1332)
                     /locus_tag="KRH54_002877"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBG8580515.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEAIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGHILNKMLEIVINNPELNEKEILKEKALNIYT"
     gene            complement(1481..3286)
                     /gene="lepA"
                     /locus_tag="KRH54_002878"
     CDS             complement(1481..3286)
                     /gene="lepA"
                     /locus_tag="KRH54_002878"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBG8580516.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            complement(3568..3819)
                     /locus_tag="KRH54_002879"
     CDS             complement(3568..3819)
                     /locus_tag="KRH54_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580517.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            complement(3850..4869)
                     /locus_tag="KRH54_002880"
     CDS             complement(3850..4869)
                     /locus_tag="KRH54_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBG8580518.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            complement(4887..5840)
                     /locus_tag="KRH54_002881"
     CDS             complement(4887..5840)
                     /locus_tag="KRH54_002881"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBG8580519.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            complement(6182..6958)
                     /locus_tag="KRH54_002882"
     CDS             complement(6182..6958)
                     /locus_tag="KRH54_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8580520.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            complement(6992..7513)
                     /locus_tag="KRH54_002883"
     CDS             complement(6992..7513)
                     /locus_tag="KRH54_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8580521.1"
                     /translation="MDIAKLILNPARLRILQYIRLYGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
BASE COUNT         2413 a         1390 c          872 g         3120 t
ORIGIN      
        1 tgtatatata tttaatgctt tttcttttag tatttctttt tcatttaact ctggattatt
       61 tatgactatt tcaagcatct tatttaaaat atgtcctata tctttacctg gttttatacc
      121 tatagatatc aaatcttttc cattaatagc caaatcttta agattaaagc actccttttg
      181 atttattata tgtttaaatt ctttttcaca ctcaataaga tttaatatct tttgttttgc
      241 atactttgga tttttggcaa gtgtatcagc ccaattaact tttattaaat cttcaaataa
      301 ttcaacatct tcaagtttat ttaataactt tttaattatc ttccttttag gttctattct
      361 ataatcatga tattgtatta atattaaaac tttattaata gtataattat catacttaag
      421 tcttttcaaa atcttctttg caatctttac agattctcta ctatgagtat aaaaatggtc
      481 aacaccattt ttatcagttg ttttagttac ttttttacct aaatcatgaa acaacattgt
      541 aagttttaaa tacagttcat cttcaattgc ttctgtagct attaaagtat gtttttgtac
      601 atcatagatg tgatatggat tgtgttgagt aacatcttct aattcacata tttcaggaat
      661 tataaatttc aacaaacctg ttgattttaa taagtttaat ttagatgaat cagatactaa
      721 tattttatta aactctgtct ggattctttc aatagaaaca ttttttatca aatctgcttt
      781 tttatgaata ctttgcttag taccttcagc tattttaaaa ttgagttgtg ctgaaaatct
      841 aactgctcta agcattctaa gtgcatcttc ttcaaatcta tctactggat ttccaacaca
      901 acgtatatac ttattttgta tatcttcata gccattaaat gggtcgacta atcctgtctt
      961 tgaattgtat gcaattgaat tgatagtaaa atctcttctt gacaagtcct taactatgtt
     1021 atttgtaaat tctattgaat ctggatgtct accatcacta taatttccat caattctata
     1081 agtcgtcact tcaaatggtt catgttcaat cataactgtt acagtcccat gttgtatacc
     1141 agttggaatt gttttttcaa ataattctac taccacttct ggtctagcac tagttgttat
     1201 atcccaatcg tttggaactc tttctaataa acaatctcta acacatcctc caactatata
     1261 agcttcataa ccattctttt ctaattcttt aattatatag tctacttttt taggaatttc
     1321 tatattaatc ataaatatca cctttttatt cttctttaat attcttactt atattctcta
     1381 tgagtttact taaaatatac cataaaaaag ctagactaat ttagaaataa ttctaaaatc
     1441 actctagctt ttaaatatct ttatctaaat ttataaggat ttactcatct aatttaagca
     1501 cagacataaa tgctttttga ggaacttcaa cagaaccaat ttgtctcata cgcttcttac
     1561 cttctttttg tttttcaaga agtttcttct tacgagatat atctccacca taacatttag
     1621 caagaacatc ttttcttaga gcgcttattg tttctcttgc aataacctta ttaccaacag
     1681 cagcttgtat tggtatagca aactgatgtc ttggaatttc tccttttaat ttctcacaca
     1741 ttgcctttcc tctagggaaa gcacgtgtct catggacaat aaaactaagt gcatccactt
     1801 gctctttatt gattaatata tcaagtttta caagtgtaga tgctacataa cctttaacct
     1861 cataatcaag agaaccataa cctctagttc ttgattttaa agcatcaaag aaatcgtaaa
     1921 caacttcatt gagaggaagg tcatagtgaa gcattactct tctatcatct atatattcca
     1981 tatttaacat atttccacgt ctttcctggc aaagctccat aacaacaccc acatagtctt
     2041 taggaactat tatatctcct tttactatag gctcttctat catctttatc tcagatggtt
     2101 ctggtaagtt tgcaggattt tgtatcatta caacctctcc atccattttt gtaactctat
     2161 atataacaga tggagctgta gtaattatat caaggttaaa ttctctctca agtctttctt
     2221 gcattatctc catatgtaaa agacctaaga aaccacatct aaaaccaaat cctagtgctg
     2281 ctgaagtctc agcttcatat tcaagtgcag catcatttac ttgaagcttt tcaagagcat
     2341 ctctaacatt ctcatacttt tctccttctc ctggatatat accacaatat accattggag
     2401 tagctttttt gtatcctggt aaaggttctt ctgtaggatt atttgaatct gttattgtat
     2461 caccaacacg acaacttctt atatctttta tactagcagc aatataacca acatcacctg
     2521 ctgataattc actaagttca gtttgacctg gagccataac accaacttca gttacctcaa
     2581 actttttatt ggtattcatc atttttattg tcataccctt tttaacagtt ccttcaaata
     2641 ctctaacata agctacaaca cctttgtaag catcatagta agaatcaaat attaatgcct
     2701 ttaatggagc ttcattatct cctttaggtg gaggaacatt ttttacaata tcttctaaaa
     2761 catcttctat gtttaaacca gttttagctg aaataagagg agcttcactt gaatcaagcc
     2821 ctattaaatc ttctatttca ttttttactt cctctggtct tgcacttggt aaatctattt
     2881 tatttattat tggtaatatt tctaaatctt ggtctatagc aagataaaca ttggctaaag
     2941 tttgtgcctc aacaccttga gctgcatcaa ctactaatag tgctccttca catgcagcta
     3001 aacttcttga tacctcatag ttaaagtcta catgtccagg tgtatctata agatttaaat
     3061 aatattcatt tccatccttc gctttataca ttaatcttat gttttgaagc tttatagtga
     3121 ttcctctttc tctttcaagg tccatattgt caagtaattg acttttcatg tctctttcac
     3181 taacaagccc agtttgttgt attaatctat cagctaaggt agactttcca tggtctatat
     3241 gtgcaattat actaaaattt ctagttctac tttgtttatt gtccacctta aaattccctc
     3301 ctcttgatta ttcttatagt tattatttta cattatctaa aaactcttta tataccaaat
     3361 ttaaaatttt tgaatataaa ctaattggca ctaaacaaaa ttatccacat tatatgtgga
     3421 ttatcaagtc taacactaaa tattatataa tatcataaac ttaaagtaaa tcctataaaa
     3481 tacagttaat gaatttttat tttatacatt aaaaatatat aactaaaata aggcttattt
     3541 taatcaataa accttattca cttattacta cttattaaat ttttttatga tttcatgtat
     3601 atcaaaattt ggtatgtcaa aatcaaattt tgttatatca aatttaatac ttggaaaatc
     3661 atattgtttc attcctagca tactttttgc attactgtcc actataaata cacaaactga
     3721 tgtacatata atcatagtaa tcaaaaataa gattctcata atatacttgt attttcttag
     3781 tttactttta ctttctatat ttttttctaa tcggctcatt tagcatgctc ctaattataa
     3841 cttaaagctc taattttttt gtttaaaata ttcatccaaa atctcggcta aatactttgt
     3901 agtagcctta gattcttcta cacttgttgc gttattccct atttctacta acatatgatt
     3961 cttaactgca aactgattga atctagcata gtctttttct actattggag atactagacc
     4021 aggatacttt ttctcagcaa aagcagttat atcttctgct aaagctctaa gttgttttct
     4081 atttgtattt ttacctccta caacaaagaa gaactttgat actctctctc catttatcat
     4141 agttgtatat tttaaatggt ctttttcctt agtggctgga ttagttaaat ccctagcatc
     4201 tctatgtaag tctatcgcta tatccactga gttatattta ggtaatatac tttgtattgt
     4261 ttttaagcta cttgcataag agttattata agatggatag tcatggtatt tagtagtatg
     4321 gactacaccc catcctttgc tatctaaagc actagttaat gcacttccta cttccattac
     4381 tgaatttttc ttatctcttg aatgataatt tccatcaggt gaatttgaat aagtctcaca
     4441 tccatgagta tgatatatca aaattcttgg attttctttg gttactctta aatcatcctt
     4501 ataatctgaa ctagatgttg tgttagtttc agttgtgtct gaattttttg actcaatatc
     4561 tggaacattt tctttaccaa cataaagttt tatatattct ttttcactct cttttttatt
     4621 atcaacttta cttgcatctt tagatttagt gtttccttct ttactttctt ccttattttc
     4681 cttacttgtt tctttatttt tattattctt tttattttct gtatcatttc cttcgacttt
     4741 agcttctgga taagatgaat ttactaaaaa ctttaaaaaa tcatcttgat tcaaagctaa
     4801 tgattttcct ggtaaaatgc atactaatat aaaagtcaag gttactactt taatgcattt
     4861 tttaaacata attgctcctc cttattttat accaatctac ctggatgtaa agacctattt
     4921 aagccttcac ttattattat agatagattt tcgattgtat catctatatc ctttggtgtt
     4981 actattaagt tcttgtcata tgggtctaac gaatctttta ttagatgata tttttcttct
     5041 tctttaagtt ttttcaacat ttcataaaaa ctttctgttt cttctgattg agctattaaa
     5101 ttgtctattg ccatatctaa gacatctatt gtaagagttg ctgcatcaac caccgttgga
     5161 acgcctattg caataacatc cacgcctaaa gtttctttag ttagagattt tctagtattg
     5221 ccaacacctc caccaggtga tattcctgct gttgatatct gtatagtaga gtttactctc
     5281 tccatttttc ttgaagccaa tgcatctatt gctactactc tatctggctt aaccttctca
     5341 accaatgatt ttactatttc actagtttct attccagtaa cacccataac tcctgggctt
     5401 aaagctgata cctcagtgaa atcatcatca tagtctttat tataattttt aaaaagatgt
     5461 ctagttacaa gtgtttttga tacactcttt ggtcctaacg catcagaagt aatatttcta
     5521 tttccaagac caattaccag ggttttttta tttttgtctt ggccaaatat atctactaac
     5581 tcctctttta aataacttat catttcttct cttgactcat catcatcaaa cttcataaga
     5641 ttactttcca gagttatata ctttcctata tctttattca ttatctctgc gccttgcctg
     5701 tctattactt caactttagt tacaatgcaa ttttctaatt ctttagtttc tatttttaca
     5761 ccaggaatac tacttccttc ttgagacttt tcacacattt cactagcttc taaagctaaa
     5821 tctgttctta cactaatcat ataaacctcc agtataaatt aaatgctaat tataattgtt
     5881 aacaaaattt agtattttat aacaagaaat tgtaaattta tttaaaacaa gtaaaattaa
     5941 ggctttcaat gtaagaaaat tgggtttagt cagtgatttg tcagtaaacc ttcttatcta
     6001 tcatttattt acaaacagaa aaaggtagca actatttata taggtgctac ctttaatcta
     6061 tttataattt tcctattaag ctttcactaa tatattaact aaaataattt tttatattta
     6121 tacctaattt atttactata taaaaaaaca gcaataatta tacaataatc acctaacacc
     6181 tttattcctt ttggaaaaat ggttctgcaa tttccaacca ttcatttgga agataaagtg
     6241 ataaataccc atgattatag ctcttagctt cataaatctc acaagagggc acataagact
     6301 gaaacagttt tgcagatttt ttgacacact tcatttcttt ttctccatac cagtacatta
     6361 cttgtgctgt ggttttttta acattctctt taagtgtata gttcatcatg taagtacgat
     6421 acatattata gaatgtttct tttcttacat gtggcatatc tgtcatataa tacgctttta
     6481 tttcatctgg atattgcata ttttcaggta acaattttgg catcatagca atttgaaagc
     6541 gacaagcctt ctcactaaac aaaagtcctc caaaaaatcg aataccagca atacaaaacc
     6601 ttgccataag aggtcttggg tagcaaatac taccatcaat aattgctttt ttagtaatat
     6661 taggttttcg agataaaagt tccatgacaa tttgaccacc aagtgatacc ccacacaaag
     6721 cgaaaagatg tccactgcaa ttttcatcta tatatgatat caacttatct gctgtatcct
     6781 ctgtagaaat gtatgttgtt tcatattctt ctccatgacc atccaatgta ggaagtatca
     6841 catgatattt gttagacaaa acttctgctt gtcgcaaata gttccaccat gcattccctc
     6901 ccccatgaat caacataata tgtggtctgt ttttatttcc aaactcatga aatttcatat
     6961 ataacctcct tttataaaaa tgggttgcat gttattttgt tttatcatta tcagctacag
     7021 gtggagaaga tataattgaa agttttctaa gttttagatt tgatttaggt ttttgttcta
     7081 tatatggttt caataaatca gctatacttt taagaagatt ttcatattca ttatcactca
     7141 tatatagtaa tgctgaagag aaaaatacat tatccttttt atggtcttgc tcttttctgc
     7201 taaagtattc attcatttcc tgcatcagac ctacataaaa tgctgtagaa agtgcaacga
     7261 aggtctctcc ttccatagat gttgtatatt cctttagtgc ataaaccttt tcaatagtac
     7321 cacgcacacg attttcttta attacttcaa tcatattgtt ctcttctaaa atttttacat
     7381 ggtgataaac tgttgcacga ggaatatcat ttaaatactt tacaatatca gatgtacgaa
     7441 cactgccata aagtcgaata tattgtaaaa tacgaagcct tgcaggattt aaaattagtt
     7501 ttgctatgtc catcttatca gccctttctt tagtctataa aaatattata gtctattttt
     7561 ttagactatt caagtctatg atataaatag taattacaac tataataaat gctacaaatg
     7621 ctattctata taaacactga cattaattac atataacctt ctaacatatt agttatattg
     7681 tttgatgcat ctatcataca tactaatgaa aaaaattatc ttgattattg gaaaaattat
     7741 cttaattaag catataaatt tatattttta taatttttca ttaaatttac aaaaa
//
LOCUS       DAEOSP010000168         7782 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.172, whole genome shotgun sequence.
ACCESSION   DAEOSP010000168 DAEOSP010000000
VERSION     DAEOSP010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.172"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            155..1213
                     /locus_tag="KRH54_002884"
     CDS             155..1213
                     /locus_tag="KRH54_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HBG8580522.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTL
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRTIFSKHYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            1317..2501
                     /locus_tag="KRH54_002885"
     CDS             1317..2501
                     /locus_tag="KRH54_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBG8580523.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHVCKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGTKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            2708..3421
                     /gene="rpsB"
                     /locus_tag="KRH54_002886"
     CDS             2708..3421
                     /gene="rpsB"
                     /locus_tag="KRH54_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBG8580524.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3500..4411
                     /locus_tag="KRH54_002887"
     CDS             3500..4411
                     /locus_tag="KRH54_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBG8580525.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4496..5200
                     /locus_tag="KRH54_002888"
     CDS             4496..5200
                     /locus_tag="KRH54_002888"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBG8580526.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            5226..5783
                     /gene="frr"
                     /locus_tag="KRH54_002889"
     CDS             5226..5783
                     /gene="frr"
                     /locus_tag="KRH54_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBG8580527.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            5786..5989
                     /locus_tag="KRH54_002890"
     CDS             5786..5989
                     /locus_tag="KRH54_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580528.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            6018..6752
                     /locus_tag="KRH54_002891"
     CDS             6018..6752
                     /locus_tag="KRH54_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBG8580529.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6765..7544
                     /locus_tag="KRH54_002892"
     CDS             6765..7544
                     /locus_tag="KRH54_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBG8580530.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         3215 a          796 c         1466 g         2305 t
ORIGIN      
        1 atatgaaaca aattaaaagc acaatgaagt agattaaaaa tttaaaacta agaatctaat
       61 atatattttg taactattta agtatataaa ataaaataat atccaaacta atacaaaata
      121 gtaataattg ttttgtactg gaggatatat atttatgaat tgtaaatgga tatctaaaat
      181 agatgagaga aaaaaatgtc atagagaagc agattcttct ggatattgta tctttcataa
      241 agaaaataag tctgatgaag agatacagtt gatgatggat actcttcata aagaagagat
      301 aagtgaattt aatggatttg tttttgaaaa tgaatttaat gcggaagaaa tattaactta
      361 caattataaa atactggatt tttctgaatc catatttaaa caaaaagcaa attttaaaaa
      421 atatattttt aaaaagaata ttatttttaa ctatacagaa tttagagata aagttttatt
      481 taatggttgt gtattcttag aaaactgtga ttttaataga accatattta gtaaacacta
      541 tataaatgac cgtatatttg aaaaagtgaa atttaaagga cctgatttgg tagttaataa
      601 agtagaaaat tttccacgaa tggatggaat aatttttagc atgtgtacaa aatttgtgtt
      661 aaagaatgtt gaatatggaa aaagtgagta tgagcatggc aagataaatt atagaatagc
      721 tagaaatcaa gcaacaaaaa ttggagaata tgagatgata ggattttact attataagga
      781 aagaatttat agtagtaaaa taatgaaacg ttctaattat cctaccttca gtgattattt
      841 agttgaaaaa ttttttgacc aaatagcacg ttacacaaca ggttatggag aaaagccatg
      901 gaatatatta ttagttatta tagcaataat aagtgttttt gctttattat acttatttgt
      961 aggcatagaa agttcaaata gcacacttgt agctttagat ataaataata taggtgatta
     1021 ctcactaagt gagattttca aaatgtatat ggatttatgg tattttagca tggctacatt
     1081 tagtacagta ggatatggag atatggttgc tacaagtttg attgggaaag cattggcagg
     1141 tatagaagta ttttttggag ttacaatagg agcaatttgg gcatctgtga ttattaaaag
     1201 aatgataagg taatatttgt gtaaatatgg ataggtatta attactagat ataaattgga
     1261 ctaaataggg aaaatttaaa tataaatata cttaataatc caagaaggtg aaaaatttga
     1321 aaaatgcact aaaaaaaatt atatcattag ccatgattct agtttttata ataccaaatt
     1381 attcatatgc agatgataaa tctgtagaca agtattcaaa aacatctata cttatagacc
     1441 aagaaacagg tagagtttta tatagtaaag aacctgatat gaaggtacca ttagctagtt
     1501 taagtaagat gatgactttt ttacttgcaa tagaagctat tgaaaataaa gaagtaaaag
     1561 aaactgataa ggtaaaaata gacaaatcta ttgcatcagt aaaagggtca agttataaac
     1621 ttaaagatgg tgaagaagtg ccacttatag agcttatgag aggtcttatg attgtatcag
     1681 gaaatgatgc ggcaattgct atagcaaaac atgtttgtaa aacgaaagaa gcatttgtag
     1741 atagaatgaa taaaaaagca aaagaaatag gaatgagtaa tacacatttt ctaaatccaa
     1801 atggtcttcc aatatacgat ttaagcaacc ccaaaaaacc agcaaaagaa aatatctcta
     1861 ctgcaaaaga tatagctatt cttggaaaat acatgtttga ccattatgaa aaacaagtaa
     1921 cagctataac tgatatggag acatatacaa attcagatag aagttttgaa aaaagcaata
     1981 caaatgcatt attaagaatt ataccagaag tagatggtat aaaaactgga tatactggca
     2041 atgctggata ttgcttatca ttttctatgg ttgtaaataa aaatgataaa aatgagaaaa
     2101 aacgtagagt aataggggtt gtattaggta ctaaccataa aaataaaaga acctctgctt
     2161 cattagctct actgaaatat ggaaaagaaa atttcaatat gaaaaaagta attgataaag
     2221 atagctttat tggtaagaaa tatataaaag gaacgaaaga gttagaggtt acattaaaag
     2281 caaaagatga actttataca atattaaaag atgatgaaag tataaagtca gaagttaaga
     2341 ttaaaaatat agagtaccct gttaaaaaag gtgatactat gggtattata aaatattata
     2401 ctaattcagg tgacttactt ggaagtgtag atatagtaag taataatagt atagataacg
     2461 tatcattaaa aacaaaatta aaaatgaaat ttgcaaatta aattcaaaaa atggtataaa
     2521 atacacaaaa aaatataaaa atagaaaata tggaaaaaat attgttattt cttcacctct
     2581 gtgatatact actagaggtg aaaaaataaa ataaatcaca cacagcattg ggatccttaa
     2641 aaggtgccta ttacatggtt ttttagggag aagatcaagc tggaggtata aaaccaggag
     2701 gtaagatatg tcagtaatat caatgaaaca attattagaa gcaggtgttc actttggtca
     2761 ccaaacaaga agatggaacc ctaaaatggc taagtatata ttcacagaga gaaatggaat
     2821 atatataatc gacttacaaa aaacagttaa aaaagttgaa gaagcttaca agttcactaa
     2881 agaagtagct gaaacaggaa agccaatctt attcgtagga actaaaaaac aagctcaaga
     2941 tgcgataaaa gatgaagctg aaagatgtgg aatgtacttt gttaatgaaa gatggttagg
     3001 tggaatgtta acaaaccaca aaactataaa aacaagaata aataaattaa gagaattaga
     3061 aaaaatggaa gaagaaggcg tatttaatgt tcttcctaaa aaagaagtta taaaattaag
     3121 agctgaaaaa gaaaaactag aaaaatactt aggtggaata aaagacatgc ctgaattacc
     3181 aggtgcaatg ttcgtagttg acccaagaaa agaaaacata gctattcaag aagctcatag
     3241 attaggtata ccagtagttg gtatagttga tactaactgt gacccagaac aattagactt
     3301 tgctatacca ggaaatgatg acgctataag agccgtaaaa ttaataactg gtgctatggc
     3361 aacagctgta attgaaggta gacaaggtgc tgaggaagaa gtagctgaag accaagaata
     3421 ataatttgac aattaaatgg taagggtcgc ccttaccatt tataatatgg aatagaataa
     3481 gtaggaggaa acttaaaaaa tggctaatat aactgctcaa atggtaaaag agttaagaga
     3541 aagtacaggc gcaggaatga tggactgcaa gaaagcttta caagaagctg aaggaaacat
     3601 ggaaaaagca gtagatttat taagagaaaa aggattatca aaagctgcta aaaaagctgg
     3661 tagagttgct gctgaaggtc tagttgcaat agaaatgaat gatgataata cagttgcttc
     3721 tatggtagaa gttaactcag agacagattt cgttgctaaa aacgaagact tcaaagtgtt
     3781 cgttaaagat gctgcttgta tggcattagc tactgataaa gaagatatag cttctttatt
     3841 aggtgaaact cataaagaag gaataacttt acaagaagtt ttaaataata gagttgcaaa
     3901 aataggtgag aaattagact ttagaagatt tgctaaagtt gtaactaatg gacaagttgc
     3961 tggatacatt cacggtggtg gaaaaatagg tgttcttgtt gaaatggaaa cagaagctag
     4021 agatgctaaa gttttagaat taggtaaaga tgtagctatg caagttgctg ctatgaatcc
     4081 taagtatgtt tcaagagatg aagtagatgc tgaatacata gcacatgaaa cagaagtatt
     4141 aactcaacaa gctttaaatg aaggaaaacc agctaatata gttgaaaaaa tggttaaagg
     4201 aagattagaa aaagaattaa aagaggtttg tttattagag caaactttcg ttaaaaatcc
     4261 agatataact gttaaacaat tagttgctga tgttgctaag gcagtaggtt ctgatataaa
     4321 agttgttaaa gttgtgagat tcgaagttgg tgaaggtata caaaagagag aagaaaactt
     4381 tgcagaagaa gttgctaagc aacttaagta ataaccaaag agagaacacg caggtgttct
     4441 cttttttaaa aaatagaaaa aattggatat gaaggattga aataggaggt ttctgatgga
     4501 taaaccaatg tacaaaaggg tattgttaaa attaagtgga gaagcattag caggagaaag
     4561 aggttttggt ataaataatg atgtagttaa tgatattgct attgctataa aaaaaataca
     4621 agaaataggt gttgaagtag ctgtagtagt aggtggagga aacttctgga gaggcagaac
     4681 cagtgaagga atggacagaa ctactgctga ctatatagga atgttagcta cagttatgaa
     4741 tgcaatggcg ctacaggatg cactagaaaa tatagatgtt gctacaagag ttcaaactgc
     4801 tatagacatg agacaaattg cagaaccata tataagaaga agagcagtta gacacttaga
     4861 aaaggaaaga gttgttatat ttggtgcagg aacaggaaac ccttacttta caacagatac
     4921 aactgctgct ttacgtgctg ctgagatgga agcagaagtt atattgttag ctaaaaatgt
     4981 tgatgcagta tatgacaagg atccaaaagt acatgcagat gctaagaaat ttacagaatt
     5041 aagttacatg gaagttatac agaaagaatt aaaagtaatg gattctacag ctacatcttt
     5101 atgtatggat aataaaattc caataaaggt atttgagctt acaacagaga atataataag
     5161 agcagtaaaa ggcgaaaata taggaactac tgtaaaataa tgaagaatga aggaggagta
     5221 aaattatgaa actggaaata cataagcaat tagaagagaa aatgaatggg acaattgatg
     5281 ctttaaagtt tgaatttgga actataagag caggaagagc taatgcacaa atgctagaca
     5341 aaataagagt tgattattat ggaactccaa ctccaataaa tcaaataggt gctatatctg
     5401 taccagaacc aagaatatta atgatatctc cttgggataa atctgctatg catgaaatag
     5461 aaaaagcgat agctaactca gacttaggat taaatccatc aaatgatggt gaggttataa
     5521 gactttctgt acctgcttta acagaagaaa gaagaaaaga attagctaaa aaagctagta
     5581 aagctgcaga agaatttaaa gttagaataa gaaatgaaag aagagacgcc aatgagaaaa
     5641 ttaaaaagat ggaaaaaggc ggagaattaa ctgaagatga attaaaaaaa gcacaagatg
     5701 aagttcaaaa aatgactgat aaatttataa aagaaataga tacattatta tctaaaaaag
     5761 aaaaggacat aatggaggta taatattgaa aaaatattat aaccttttag gtcttcatgt
     5821 agatgaggta aaacaatatt ttgatgagca aaatataaat tataccgtaa aatctattga
     5881 aggtaaaaag gataaagaaa aacttgtagt tcctagagtt ataaaaatat ctgagatagg
     5941 tgatagtgta gaactgataa tcacatactt ttctgactcc ttagtttaag gagtcagaac
     6001 aatattggag gcaaaatatg aataacaaca ttatatatga tatagactta aataacatac
     6061 ctactcatat tgctattata atggatggaa atggaagatg ggcaaaagct agatttcttc
     6121 caagaactgc aggacataag gctggagtag aaactataag agatatagta aaagaatgct
     6181 caaaattagg tgttaagcac ttaacattat atgcattttc aacagaaaac tggaaaagac
     6241 cgaaacttga agttgacaca cttatgaact tattatctac atacttaaga aatgaaatag
     6301 ctgaacttca ccaaaacaat gtaaaagtaa ctgctatagg agatataagt gctttaccaa
     6361 aaacttgtat aagagaatta aactcagcta aggaactgac aaaaaacaat acaggagtta
     6421 acttaaattt agcattaaac tacggaagta gagcagatat aaaaaatgct cttatagata
     6481 tagtaaaaaa ttgtgaaagt ggtaaaatag atataaacaa tattgatgaa gatattataa
     6541 aaaactattt aagcacaaag tctataccag atccagactt ggtaataaga acaagtggtg
     6601 aacaaagatt gagtaacttt ttactttggg aagttgctta ttctgagttt tactttactg
     6661 atatacattg gcctgatttt aaaaaagaag aactacagaa ggctatatac gtatatcaaa
     6721 gaagggatag aagatttgga ggacttaagt aaggagatta ttttatgctt acaagaatta
     6781 ttgcttcact agctctggtt ccattatttc tatttgttgt atatggaggt attcctctat
     6841 atatagctga gacagcaata gtttatattg cattgcatga gttttataag gcatttaaaa
     6901 taaaagatgt acatccaata ttcattatag gatatttatt ttctatttat ttagctgtaa
     6961 aaaatatttt taatttacca ttagaatata cttatgcagt aatttttatt ttatttttag
     7021 caagtataat ttatatgttg atgggcaaaa acaatgttat agatgtttcg attacgtttt
     7081 taggagtttt ttatatagga gtatttttag attttataat tataactata aatggatttg
     7141 aaaaaggtag tatatatgta tggcttatat ttgtgatatc ttttatgaca gatatatttg
     7201 catattttag tggttattta cttggaaaac ataagctaat tcctaaagtt agtcctaaaa
     7261 agactataga aggagctatt ggaggaataa taggaagtac actttgttgt attttatttg
     7321 gatatttatt tggcattgat ttgcttcaat tagcgataat aggaagtata ggaagtgtaa
     7381 tagctcaatt aggagattta tttgcatctt ctataaagag atatgtaggg ataaaggatt
     7441 atggaaagat aatacctggt catgggggta tattagatag atttgatagt gttatattag
     7501 ttgcaccttt tgtatatagt gcgattaaat tttttattag ataaagattg gttggattta
     7561 aaagagcaat ctataattgc tagtaatgaa aatagctatg taacttttaa atgcagagat
     7621 attctgcagt tattagttac atggctattt tcattgttca aaaatttaga agaccttggg
     7681 gaattgtata agaataggta ttactttata atagtatact atatttgtag tgagtatatt
     7741 ttttatacta taactatcat taaaaaatat ttttttgttt aa
//
LOCUS       DAEOSP010000169         7696 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.173, whole genome shotgun sequence.
ACCESSION   DAEOSP010000169 DAEOSP010000000
VERSION     DAEOSP010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7696
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.173"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            95..2323
                     /locus_tag="KRH54_002893"
     CDS             95..2323
                     /locus_tag="KRH54_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBG8580531.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
     gene            complement(2561..2860)
                     /locus_tag="KRH54_002894"
     CDS             complement(2561..2860)
                     /locus_tag="KRH54_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBG8580532.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            3067..6108
                     /locus_tag="KRH54_002895"
     CDS             3067..6108
                     /locus_tag="KRH54_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBG8580533.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTPENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGET
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            complement(6269..7543)
                     /locus_tag="KRH54_002896"
     CDS             complement(6269..7543)
                     /locus_tag="KRH54_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG8580534.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
BASE COUNT         2844 a         1073 c         1102 g         2677 t
ORIGIN      
        1 gacaaaattt taactttact atttgtgtaa aaagtaatat aatttaaaat tgattataaa
       61 tctaactttt ttctttatca aaatatttta ggaggtgaga catttgaaaa aaactaatag
      121 tttaaaagaa attttaaact ggaataatat caagaattta agaccttcaa agggtttaaa
      181 aaatatatta gaaattttta aaagtgacct aaaagatata cgtggcaatt atgccgtact
      241 tgtaatagtt ttaggtcttg cttttttacc atctctgtat gcttggttca atataaaggc
      301 atcttgggac ccttatggaa gcactgccaa tatgcttgta gcagtaacaa atgaagataa
      361 aggaaatgaa atagcagggc aaaagattaa tgtaggagac caattaattg aacaactcaa
      421 agataacaag agtttaggtt ggcaatttgt agatgaaaaa actgctcttg aaggtgttaa
      481 aaaaggtact tattatgcat cacttaagat tccaaaaaat tttacaaaag atttgacatc
      541 tttaattaca gatgatgtta aaaaaggcga aattatctat actgttaatg aaaaaataaa
      601 tgcagttgca ccaaagatta cagataaagg tgcttctaca atacaactac aggttaatca
      661 gactatagta aaaacagtta gtgaaatcgt tcttggtata tcaaacggag ttggtgcagg
      721 aattgaacac agtcttccaa aattgacaaa agttgaaaat tcccttattg accttcaaaa
      781 gagatttgac catataaata agactgttga tttagcttct gatgctactt caaaattatc
      841 tgatacagtt aaagatatta aatctgattt acctacaatt aaaaaaactc tgaatgatac
      901 aaaactatta tcaagtgact taaaaaaatt cttagaggat acaaatgata atttagacga
      961 actttctcct cttattaagt ctgacttaaa tttaatggtt gacctttcat cttctgcttc
     1021 ttctcttaca ttaaatttaa ttgatgcagt aaatagtggc agtgaagatg tacctaagtt
     1081 gatagataat ttatcagaaa aactttctaa tttgcaatct ttaaatgata ctttggtgga
     1141 ttttttaact aaattaaacc aacttacatc aaataataga ttggatgatg tcatagataa
     1201 tcttgaagac tctagcaata aaatagattc atctatatca acattaaatg atattaaaaa
     1261 caaagttata tctggacaac aaccatctat aagtgctttg aataatgttt taagtctatc
     1321 aaatggtatt ggaagaatta atttaaatat tttaaataat tttgactcaa aaatatcaaa
     1381 acctataaat agtatatttg caaacagtat aaaagtagca aatgatataa ttactgtttt
     1441 agataaggca gaagcaaaac ttccaaaggt tgaagaaata cttactactt ctctaaaact
     1501 ttcaggaaat gcacaggaaa gtatatcatt gataagagaa agacttcctt tagcaaaagg
     1561 aatgttggat gatttgattg atactttaag caaaattagt aatggtgaag atatgaaaaa
     1621 attagtcagc ttgcttgaaa gtgatatatt gacaaagtct aatttcttaa aagaacctgt
     1681 tgaggtgaaa acagagaaat tatatccaat tgaaaattat ggttctgcaa tgacaccatt
     1741 ttatagtgtt ctttctacat gggttggtat attacttatt tcagcactat tgtctacatc
     1801 tgtacatgga aactataaac cttatgaaat ttactttgga agaggcctta cttttttaag
     1861 tatagctctt gtccaaggtt ttataatagg aactggagat atattattgt taggagtaac
     1921 agcaaaacat ccaattttat tgatagttct gcttatgttt actagtatgg tatttaactt
     1981 tatagtgtat tctttagttt ctgtatttgg aaatgtagga aaagcaattg caatagtact
     2041 attagtcgtt cagattggtt cttctggtgg tacattccct atacaagtta cacctacatt
     2101 cttccaaaga ataaacccta taatgccatt tacctatgca ataggtgcta caagggaagc
     2161 agttggtggt atatatgctc caaatctaac taaagatata ctagttctat tactatttat
     2221 gattctgtct atttgtttaa acgtacttct aaaaggacct ataaatagag ttataaaacc
     2281 atttaatgat aaatttggcg atagtaattt aactggtcat taaaaaatta attctgtctt
     2341 taaatatatt tttttaataa tattctaagt taatattaaa aggtagtagc taaaaagcta
     2401 ctaccttttt ccaacttaaa aactaatatt tactacaaaa actgtcatag aaaaaaacga
     2461 aaaaattcgt tctttcctaa atttatctta taaatattta gatatattcc tacttaataa
     2521 gcagagctat acatagtatt taatgtataa tttacattat ttaactcata taatcaatta
     2581 ttcctgagtt taaattatat aaatgctcaa aaccttcttc agctaacaaa ttacatgcag
     2641 taacacttct atgaccactt ctgcaataaa ctactacttt cttatcttta taatcgtaaa
     2701 tttcatctat attattgatt aactcttgta aagatatatt tatagcattt tctattttat
     2761 attccttaaa ttcatcttta gttcttacat ctaataataa aacatccttt tcatttttca
     2821 tcatttcttt aagttgcttc ttatttatat tcttatacat atgtctaaaa ttcctccatt
     2881 ttatttatat attacctata ccctttattc tttatacaac aacaaatatt atttttatat
     2941 ttttctctgt tatataaaaa gctatactta taaaagcttt ttttattgat aataatctgt
     3001 tgattaaaat aattaagaat gttatgctaa ttataaatta ttaaaaaggg aaagaggtaa
     3061 tgtaatatgt ttaagcaaag gctctctaaa ttattatcta gtacattagt attgagtatg
     3121 ttatttactg ctgctccaaa tattactttt gctgataata caaaagataa ttccgaaaaa
     3181 tatcaaagtt ctgatataga attacatgat tatagtaaaa acgcggaatc ttacacaaaa
     3241 actaaagctc ttgctaaaga gaaaatccaa actttgctat caaagtatgg tgctgtaagt
     3301 gctcaatatg ctttgattga taatggcaaa attgagattt ctggtaatgg tggcgtttac
     3361 agcaaacagg ataacaaaaa cttaaataag gataatatgt atagtatcgc atctatcagt
     3421 aaaatgttta ctacaacagc tgttatgaag ttggttgatg atggaaaatt aaatttagat
     3481 actcctgttg taaagtatat acctgaattt aaaatggctg atgatagata caaggaaatc
     3541 actccaagaa tgttattaaa tcattcatca gggttgatgg gaagtagttt taaaaacact
     3601 attttattag cagacaatga ttcttatggt catgataatt tcttaaaaga attacaaaaa
     3661 caaagattaa aagctaaacc aggagctttt tctgtttatt gtaatgatgg atttacttta
     3721 gctgaaattt tagtagaaag agtatctggt atgtcattta ctaattttct tgataaatac
     3781 ataaataatc cacttaactt acaaaataca aaaactccag aaaatagttt tgatagttca
     3841 aaactagcaa aagcatatgt accttattgg gaagatgcag ttccacaaga taaccttaat
     3901 gctataggtg caggtggttt atattcaagt gcagaaaatc tatgtacatt tgcacaaaca
     3961 tttatgaaaa attcaaatgg tattctttct cctgcttcag taaaagctat ggaaaacaaa
     4021 gaatatttaa atggtctatg gcctgaagga gaagactcta tacttggata tggtttaggg
     4081 tgggattgtg tcaacactta tccatttaat cagtataatt taaaagctct tacaaaaggt
     4141 ggagattctt tattattcca cagtaatctt atagttcttc cagatgaaaa tatggcagtt
     4201 gcagttcttt cttctggtgg aagtagtcaa cttaatgaaa taattggaca agaaatctta
     4261 ctatctgcat taaaagaaaa aggtaaaatt aaagaaataa aacctgataa aacatttagt
     4321 aagccacaac aagttaaaat gcctagctct ttaaaagaaa atagtggtct ttatgcttct
     4381 tcaaatatga taaaagtaga tgttaatgat aatggtactt taacagtatc ttctccttat
     4441 atagaaaatg gacctgaaga taaatacgta tacataggtc aagatagatt tgtatctgaa
     4501 aaaggtaata gttgtttaaa atttgtaaaa gaaaaaaata atatcacata tttaaatatg
     4561 agctcttatg atgatgtccc tggattaggt cagacagcaa gtttatacta tgtagctcaa
     4621 aaagtagatg acaacaatat atcaaatagt gtcaaagaag tgtggaaaaa aagaagtggc
     4681 aaaggttact atctagtgga tgaaaaatat acttctcaaa gctatatgtt tggctctgta
     4741 aaagcaagtt ttagtctttc tgatgaaact ccaggttata tagttaacac taaaataatg
     4801 gatgagaata actcaaatgc ttttatagaa atacctggtg taataggtcg tgatttatcc
     4861 gacattaaac ttcataaaga aaatggtaca gagtatttat cttttggaac actaacgtat
     4921 gttagtgaag attctataac taatttgcca gcagaaaaat cttttacttg tgaactagaa
     4981 tccaatggtt atgcaaaatg gtataagatt ggtgacgata tcgcaaataa aaaaatagaa
     5041 gttaatcttc ctcaaaattc agcttttgct gtttatgatg ataaaggtgt tcctgtaaac
     5101 tattctttag ttacaaagaa caatagagtt agattgccaa aaggaggcgt aattgttttc
     5161 ttaggaagtc caaatgctag atttgaggtt acatatcaag atgaagtaaa tgcaagtgca
     5221 cttacaggaa ctgatagata tgaaacttct attaagataa gtcaagcagg ttgggaaaat
     5281 gctgaaaatg cagttttaat aaacgattca gcaattgcag atgctttggc tgcaacacca
     5341 tttgcataca agaaaaacgc tcctatatta ttgactggaa gctctcaaat aaatgaaaaa
     5401 actttggctg aattaaagag attaaaagtt aaaaatgtat atgtagttgg tggagaaact
     5461 tctataaatg aaaaatcttt agatactata aaatcaaaca atatctctgt atctagaatt
     5521 tcaggaagtg atagatatca aacttctatg aacattgcta aagaattgaa caatattagc
     5581 aatatatcaa agatttcagt agttaatggc gaaaaaggat tagccgatgc agtaagcatt
     5641 ggagctgtat cagctcaaaa tgatatgcct ataatactta ctaatgaaaa tagtaatata
     5701 actgaaataa ataacttgtt taaaaacaaa aagattgata aatcttatgt aatcggtgga
     5761 gaatatacag tttctaaaaa tatagaaagt aaacttcaaa atccacagag aatatcaggt
     5821 agcactagaa atgaaactaa tgcaaaagta ataaaagaat tttacaagga ttctaagata
     5881 gacaatttat atgttgctaa aaatggaatg aataaacaag atgatttaat agacggatta
     5941 tctgttggag ttttggctgg aaaaactaaa tctcctgtaa tgctagtagg aaactcacta
     6001 gattataatc aaaaagaact atttaaaact atgagattta aatctgtaac acaaattggt
     6061 ggaaatggaa atgaaaactc atttaagcaa ataaaagaaa tagcttaaca taactaaaaa
     6121 aggctgtatt caagtaatat taattgaata tagccttttt ttatttaagt tataagtttt
     6181 atatgtgtaa aaattaaatt taaatattaa atgataagtt tattaagata tagataagtc
     6241 taaaactttg ccttttttaa gttagtttct atttatcatt catacttaac aatttatcta
     6301 aatatactga ctttaattgt ttactggcta aaatcttttt agctggttca agctttaata
     6361 tagctgacag tatagctccc atagctctat gactagccag agcattatta tcaaaaatta
     6421 aattttgtaa ctgccctttt tcaattgcca taagaactgc tctatgtact gaatttgctg
     6481 gagttattat tttttcatct ctctttaata acataatgct atttttatgg caatttctaa
     6541 cacaaactcc acaacctaga catctatcct catcaatttt tatatattct ttaccatctt
     6601 ccttaacctt gcttatcgca tctattggac atgcagttat acatttacca cactttacac
     6661 aattttcatg gtttatattt ggtataaaac ttgttgtctg tactggatga agatttccaa
     6721 atcttttagc tgctaccatt gcttcacagc aacatccaca gcaattacat ataaacgtaa
     6781 cccctttcct aacattttct ccacattgaa ctaagttatg ttcatatgct tgatgaagta
     6841 gttctttaca ttctatttta tccacacgtc ttgcaaattt attatttatt aaagaatttg
     6901 ctacattgtc aaacgtcata catatatcca taggagcatc acaagctttt ccaacatgtt
     6961 gcattctatg tctacagtaa cacatactta tacctatatg agttgattca tcaattatat
     7021 gagtagccct ttcataatct aaaatactaa cctcattatc attagttaaa acttcctctt
     7081 gaacgtatac tcttccaagc ttcgtttctg ttgaataaaa taaatctttg ataaaatctt
     7141 cttctacatt catatattga tagtaaagct ctgccaaaag tttttggtct atatcgtgtc
     7201 tcgttctcat catggcaaac tcaaaaaaac cagccattgg aggaggcatt atatatttct
     7261 tccctttatt atcttctata tctagtatca gagccttact agcaagttta tccaatactc
     7321 tatatgcttc actttcactt acactccaaa ttttagctgc tgtttttact ctgaaaggct
     7381 ttattggaag ctgagcaact aacttcgctt cttgttctgt atatagtaca tttagtattt
     7441 tgtataatgt atcagatggt ggagcacctt gtggaaactt gtttattctt tcctctaaac
     7501 ttttatacat attcttcgca gttatatgag aaatagtaat caccttcttt ttcttttgtt
     7561 ttccaataaa atcaaatatt ctttcttatt atatattctc ttactttata taataccaat
     7621 tctttctaaa tagttaaata cctctagtaa tttattatta gatttcttat ataaataaaa
     7681 aatcatttaa gtgata
//
LOCUS       DAEOSP010000170         7531 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.174, whole genome shotgun sequence.
ACCESSION   DAEOSP010000170 DAEOSP010000000
VERSION     DAEOSP010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.174"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            269..1750
                     /locus_tag="KRH54_002897"
     CDS             269..1750
                     /locus_tag="KRH54_002897"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBG8580535.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            1971..2126
                     /locus_tag="KRH54_002898"
     CDS             1971..2126
                     /locus_tag="KRH54_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580536.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            2274..3671
                     /locus_tag="KRH54_002899"
     CDS             2274..3671
                     /locus_tag="KRH54_002899"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBG8580537.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            3656..4069
                     /locus_tag="KRH54_002900"
     CDS             3656..4069
                     /locus_tag="KRH54_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBG8580538.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            4175..4933
                     /locus_tag="KRH54_002901"
     CDS             4175..4933
                     /locus_tag="KRH54_002901"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBG8580539.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            4946..5683
                     /gene="rlmB"
                     /locus_tag="KRH54_002902"
     CDS             4946..5683
                     /gene="rlmB"
                     /locus_tag="KRH54_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBG8580540.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            5686..6213
                     /locus_tag="KRH54_002903"
     CDS             5686..6213
                     /locus_tag="KRH54_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBG8580541.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            6278..6925
                     /gene="sigH"
                     /locus_tag="KRH54_002904"
     CDS             6278..6925
                     /gene="sigH"
                     /locus_tag="KRH54_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBG8580542.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            6999..>7531
                     /locus_tag="KRH54_002905"
     CDS             6999..>7531
                     /locus_tag="KRH54_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBG8580543.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSAL"
BASE COUNT         3170 a          761 c         1452 g         2148 t
ORIGIN      
        1 aagcaaaata ttttgttata tagattttca ataaagcaaa caataaatat aaatgcatat
       61 taataaggag tgcttttaat ttttagaaaa taaatgactt aaaagaaaga tttatgaaat
      121 taaaactagg attttctgat ataataaaca tgtgtatttt taaataaaaa ggatattatt
      181 attagatttg attaaaaata aacttgcaaa acaatgtact agtttaatat tatagttata
      241 ttgaataaaa tatcaggagg aagtaaaaat gaatgtaaga gtaaggtttg caccaagtcc
      301 aacaggattt gtacatatag gtagtttgag aactgcttta tataactatt tatttgcaaa
      361 aaaaatgggc ggagaatata tattaagagt ggaagataca gaccaaagta gattagtaga
      421 aggtgctata gagaatatgc ttaatgctat gaaatgggct ggggtcaacc atagtgaagg
      481 tgttatatta gatgatagtg gaaaagtagt acaaaaagga gaatatggtc catatattca
      541 atctcaaaga ttagatatat accaagaata tataaagcaa cttttagata gtggaaaagc
      601 ttattactgt ttctgtacaa aagaaagact ggatgaagta agagatgctc aaagagcagc
      661 aggagaaaca gctaaatatg atggtcattg taagaatctt tctaaagaag aagtagaagc
      721 gaatataaaa gcaggaatac catatgttat aagattaaga ttgccagaga atcatacaat
      781 taagtttaca gacttagtta gaggagatat ggaatttaat actaatgact tagatgacca
      841 agtattaatg aaaacagatg gatttccaac ttatcatttt gcagtagttg ttgatgacta
      901 tttgatgaaa attacacatg ttataagagg agaagaatgg gtatcatcta ctccaaaaca
      961 tgtttattta tatgaagctt ttggatggga agcacctgta tttgtgcatt taccaaatat
     1021 acttaataaa gaaaagaaaa aattaagtaa aagacaaggt gatgtagctg ttgaagactt
     1081 taagaaaaaa ggatatttac cagagggatt agtaaactat gtagcattag ttggttggtc
     1141 tcctgaagat aatcaagaat tgtttacaat ggaggaatta gaaaaagcct tctctgtaga
     1201 aagagtatct aaaagtggtg gagtatttga tactgaaaaa ttaaattggg ttaatcagca
     1261 ctatataaaa gatggtgatg atgcttatct aacagactta gcaataccat ttttaataga
     1321 agatggattt ataacagaag aagaagctac taataaatat gatttcttaa agagtatgat
     1381 atcagtactt aaagagaaat tacaatatgt aaaagaagtt actgaacatg caagtatatt
     1441 ttttggagat aaaatagagg tagaaactga agagggaaga gagttcttaa gattagaaca
     1501 tatacctact ttaatagatg ctttgagaga aaaaatagaa aaaacagagg tattaaatgc
     1561 tgagtttgtt caagctatgc ttaaagaaat acaaaaagag tacaaaataa aagggaaaaa
     1621 tttatttatg ggctcaagaa taattttgac agggcaaatg catggtccag atttaccaaa
     1681 agtaatggag gttttaggaa aagaaacttg cttaaataga atagcttatg taaaaaataa
     1741 tatattatag tcataaaagt ccgctaattt agaagcggac tttttttgtg ttagtatttg
     1801 gtgaaaatat ctgcttaaga aaatgaaggt atttaaggta ataaatttaa tatataaaaa
     1861 gcaaagcaaa aatataaata aaatatgaat aataaactaa taaaatatta agcatagaag
     1921 aagggaatct attattttag tttgatttaa ttattaaggg ggtaattact atgtatagta
     1981 atattgatga tgttaaaaag gaattaaagg agctatgctt agaatatgtt acaattttag
     2041 aaaaattaaa agatgaaaaa atgattacag aagaaacttt tgaaaagtgc tcttctcaaa
     2101 aaaaaatatt tttagaagaa caatagagga tggtaatagt attattatta ttatgtagtt
     2161 tgaaagatag tacgaaaaat gatagtaata ctattattta ttgcaatttt caactataat
     2221 ataatatata atgttaagaa gataatttat aaaaatacag tgaggtgatg agtgtgaaag
     2281 tttataatac attgactaga acaaaagaag aatttgttcc attagaagaa ggaaaggtaa
     2341 aaatgtatgt gtgtggtcca acagtttata actatataca cataggtaat gcaagaccat
     2401 ttataatatt tgatactcta agaaggtatt tagagtatag aggatatgat gtaacttatg
     2461 tacaaaactt tacagatgtt gatgataaga ttataaatag aagtcatgag gagggaatat
     2521 ctcctgaaga agttgcggct aagtatataa aagaatattt tgtagactgt gatgggctag
     2581 gaataaaaag agctactgtt catcctcagg ttacagataa tatacaacag attatagagt
     2641 ttataaaaga gttggaagat aagggatatg catatgcagt aaatggtgat gtatattttg
     2701 atacaaataa gtttgaaggt tatggaaaac tttcaggtca aaaacaagaa gatttagagg
     2761 caggggctag aattgaggtt aatgaccaaa aaagacatcc tatggacttt gttctttgga
     2821 aggcaaaaaa agaaggtgag ccaggatggg atagcccttg gggagaaggt agaccaggat
     2881 ggcatataga atgttctgtt atgtctaaga gatatttagg tgaaacaatt gatatacatg
     2941 caggtgggca agatttaaca ttcccacatc atgaaaatga aatagctcaa agtgaagcta
     3001 gaagtggaaa aacattctca aaatactgga tgcataatgg atatataaat ataaatgatg
     3061 aaaagatgag taaatcaaaa ggtaatttct ttacagtaag agatatatca aaattatatg
     3121 atttagagat agtaagattt ttcatgttat cagcacatta tagaaatcca gtaaacttta
     3181 gtgatgagat gttaaatcaa gctaaagcag gtcttgaaag actatataat actaaagaaa
     3241 aattagaatt tacgctaagt aatttagtag aatctccttt aacagaaaaa gaagtagagc
     3301 ttgttaaaga attagatgac tttagacaaa aattcataga tgctatggat gatgatgtta
     3361 atacagcaga cgcagtaagt gtaatatttg aattagctaa gttaataaat tctaatgtag
     3421 atgaaaattc ttctttggag tttgctaaaa aatgcttaga tgaatttaat gaacttacag
     3481 gagtattaaa tattgtaaat aagaaaaagg atactgtatt agataaagac attgaagaat
     3541 tgatacaaaa aagaactgat gcaaagaaaa ataaagagtt tcaattagct gatgatatta
     3601 gacaacaatt actagatatg ggaattgtat tagaagacac aagacaaggt gttaagtgga
     3661 aaaggatata aaaatggaaa aaactgaatt agttactatg tcaccattag ttttagcata
     3721 cttaggtgat acagtgtatg agacatatat aagagaatat ttaattagac agaatacaca
     3781 aagaaaagtt aatgatttgc ataaactagc tataaagtat gtaaaagcta aagctcaggc
     3841 aactataatt catgaaattg aaattgagtt aactgaagaa gagagtaaaa tttataaaag
     3901 gggaagaaat caaaagtcaa atacttctcc taagaatgca gatattatag actacaaaca
     3961 tgcaactggg tttgaggcat tagttggata tttgtattta aataatgaaa tagagagact
     4021 tcaatatatt atcaataaag gaatcaaaat tattgaaaga gatatgtaaa tattaaagag
     4081 tgttgtctta ataataagta ataaggcaac acttttttta taactaatgt ataataaata
     4141 tatagaaatt agtaatgaga aaggataaaa agttatgaaa gtaaaattaa tatcacatac
     4201 acctgaacca gaaaaagtaa tagctatggc agctaagcta tgttattcac cagttgggac
     4261 agacgaaata gaaaaagact taacagatga aagtatagag aaatttttaa atatgttatt
     4321 aagtataggt catgggtcaa tattagaaca tgcttcattt acattttcaa tagaaggtat
     4381 ttcaagggct tgttcacatc aaattgttag acatcgtata gctagttttt cacaacaaag
     4441 tcaaagatat gttaaattag aacaatttga gtacataatt cctccagaaa tagaaaaaat
     4501 agaaaaggca aaagagttat ttatagactc catgaaaaaa gaccaagaga attatgataa
     4561 gttagttgaa atattgtttg aaaatcatta taatgaccta ataaaaaacg gtaaaaatga
     4621 gaaaacagct aaaagacaag ctgaaaagaa agcaatagaa gatgctagat acgtatttcc
     4681 aaatgcatgt gaaacaaaaa tggtatttac tataaatgct agaagtttgt ttaatttctt
     4741 tgaacataga tgctgtgaaa gagcacagtg ggaaataaga aatttagctg tggagatgtt
     4801 aagagaggtt aaaaaggttg ctcctatatt atttaaaaag actggtccaa gttgtgtaaa
     4861 tggaagttgt ccagaaggaa ctatgacttg tggagatata gttcaagtta gagaaaaatt
     4921 taaggccttg taggaggtga ataatttggc gagtatagaa ggaagaaatc cggtaataga
     4981 agcaataaaa agcgacagag aaatagataa aatattaatt gcaaattcag ctaaagaagg
     5041 ttcaattaag aaaataatag gaatggctaa agataagaat ataattattc agtatgttga
     5101 taaacataag ttagatgaag taagtacaag tcattcacat caaggtgtaa tagcttatgc
     5161 aagtgagtat aagtattatg aattagatga gctaatagat ttagcaaaga ataaagatga
     5221 agacccattt ttcattatat tagatgagat aacagaccca cataatttgg gttcaataat
     5281 aaggacagca gatgcagtag gagcacatgg agttatcatt ccaaaaagaa ggtctgtaca
     5341 cataactcct gtagttgcca aagcatctgc tggagcagta gaatatatgc cagtttgcaa
     5401 ggtaaccaat atagttaata caataaaaag actcaaagaa gaagggctgt ggattgctgc
     5461 agctgatatg gatggagaaa ctttttataa acaaaatctt actggtcctc ttggtgtagt
     5521 aattggaagt gaaggttttg gaatatctag attggttaaa caaaactgtg actttatagt
     5581 aaaaatgcca atgataggta atgttacatc tttaaatgca tctgtagcag gtggaatact
     5641 tttatatgag atattcagac aaagactgga taaaagtaaa taggcatgaa aaaaaatata
     5701 aatcattact taataataga tggctacaat attataaatg cgtgggacaa tttgaaagaa
     5761 ctggctaaag aggacttaga agattctcga gaaaagctta ttgatgacat tatagaattt
     5821 tctgagttta tgggttataa gactataata gtttttgatg cttataatgt taaaaattct
     5881 cgagaaaaag ttgaaaaaag aaaacacata acaatagttt atacaagaga acaccaaaca
     5941 gcagatagtt atatagagaa atttataact tctttatcaa agtatgatga tgtaaaagta
     6001 gccactaatg actatgcaga acagcaaatg attttaggaa aaggtgctac tagaatgtct
     6061 gcaagagaat taaagttaga gttagatcgt tccaagaata aaatgaagga aaaaaatatc
     6121 ggtttgagga aaaaaattca gcgaaattgg ttggaggaaa ggttagataa agaaacattg
     6181 tcgaaacttg agaacattcg tagaaagcgt tgaaattact aggttcttta gactataata
     6241 tacctataaa tttgttttat atatggggga gatttacatg ttggtagcaa aagaaaaaag
     6301 ttatgagtta gtggacaatt gtcagcagga tgagtataac attgtattaa gagcaagtga
     6361 aggggataaa atagcactag agtacattat tactaaatac agaaattttg taaaggcaaa
     6421 agcaaagtca tattttttaa ttggagcaga taaagaagat ataatacaag aaggaatgat
     6481 aggcctttat aaggcagtta gagattttga tggaagtaag accaattcat ttaaatgttt
     6541 tgcggagata tgtataacaa gacaaataat aactgctata aaaactgcaa caagacaaaa
     6601 acatatacct ttaaattcat atgtttcttt gaataaacca atatatgatg aagagtcaga
     6661 cagaacactt ttagatatta tagctacaag tatagtaaca gatccagaag agcttataat
     6721 tagtaaagaa gagttaaaaa atatagaatc aaaaatgaac gaattgttaa gtgacttgga
     6781 attagaagta ctagaattat atttaaatgg aaagtcatac cagtttatag cggacaagct
     6841 taaaagagat gtaaagtcaa tagataacgc tttacaaagg gttaagagaa aattagaaaa
     6901 acatcttgaa aacagaaatg attaatagta aaatacttac cataagctta ttgtgagcaa
     6961 aattaattta tatcgaaaga taagggagga aattcaaaat ggctaaagct aaatacgaaa
     7021 gaacaaaacc tcatgttaat atagggacaa taggacacgt agaccacggt aaaactacat
     7081 taacagcagc aataacaaaa acattatatg acagatatca attaggagaa gcagtagatt
     7141 tcgcaaacat agataaagct ccagaagaaa gagaaagagg aatcacaata tcaacagcac
     7201 acgttgagta tgaaacacca aatagacact atgcacacgt tgactgccca ggacatgctg
     7261 actacgttaa gaacatgata acaggagcag cacaaatgga cggagcaata ttagtttgtt
     7321 cagcaacaga tggaccaatg ccacaaacaa gagagcatat actattatca agacaagttg
     7381 gagtaccata tatagtagta ttcttaaaca aatgtgacat ggtagatgat gaagagttat
     7441 tagagttagt agagatggaa gtaagagatt tattaacaga atatgatttc ccaggagatg
     7501 acactccaat agtaagaggt tcagcattaa t
//
LOCUS       DAEOSP010000171         7463 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.175, whole genome shotgun sequence.
ACCESSION   DAEOSP010000171 DAEOSP010000000
VERSION     DAEOSP010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.175"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            42..1019
                     /locus_tag="KRH54_002906"
     CDS             42..1019
                     /locus_tag="KRH54_002906"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBG8580544.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPNMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1012..1980
                     /locus_tag="KRH54_002907"
     CDS             1012..1980
                     /locus_tag="KRH54_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBG8580545.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLVVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2163..2753
                     /locus_tag="KRH54_002908"
     CDS             2163..2753
                     /locus_tag="KRH54_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG8580546.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2772..3542
                     /locus_tag="KRH54_002909"
     CDS             2772..3542
                     /locus_tag="KRH54_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBG8580547.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3679..4647
                     /gene="dusB"
                     /locus_tag="KRH54_002910"
     CDS             3679..4647
                     /gene="dusB"
                     /locus_tag="KRH54_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBG8580548.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4756..5235
                     /gene="greA"
                     /locus_tag="KRH54_002911"
     CDS             4756..5235
                     /gene="greA"
                     /locus_tag="KRH54_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBG8580549.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5250..6779
                     /gene="lysS"
                     /locus_tag="KRH54_002912"
     CDS             5250..6779
                     /gene="lysS"
                     /locus_tag="KRH54_002912"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBG8580550.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7088..7282
                     /locus_tag="KRH54_002913"
     CDS             7088..7282
                     /locus_tag="KRH54_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580551.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
BASE COUNT         2972 a          759 c         1514 g         2218 t
ORIGIN      
        1 agaaaataag tcatgaaatt atgtcatatt agaggtgaaa tatgagagat aaaataatag
       61 agattatatt aaataataaa tctgaattta tttcaggaga agaattatca aaacaattag
      121 gggtgtcacg tgcagctata tggaaacata tgaaagcctt aaaagaagag ggatataata
      181 tagaatctgt aaataaaaaa gggtatcgat tagcggaaaa tccaactgat ttattaagcc
      241 cacagaatat ttactataaa ttagatacag agtttattgg aaaaaatatt gaacatcttg
      301 acactataga ttcaacaaat gaacatgcta aaaggatagc tataaatagt gtagatggga
      361 ctgtgatttt aagtgaacat caagacatgg ggaaaggacg attgggaaga agatgggatt
      421 caaaacctca tgaaggaatc tggatgagta ttatattaaa accaaatatg gaaccattca
      481 aagctccatt tataacttta atagcaggag caagtgtaac gaaagcttta aacaatctag
      541 gagcaaatac attaataaaa tggccaaatg atgttatttt aaatggaaaa aaaatatgtg
      601 gtatattaac agagttatct gctgaaattg aaagaacaaa ttatgttgtt cttggaattg
      661 gaataaatgt taaaataatg gattttgaac aagaaatagc tgacaaagcc acatcattgt
      721 ataaagaagg gtatatgtta tccagagtag atattgttag gaatgtattg actgaatttg
      781 aaaaattata tttagattat actgtaaata attccaaaga aaaaacctta aaaatatgta
      841 gagattattc tgcaattata gataaaaata tatatgtatt aaaaggagat aatagagaac
      901 tagtgaaatg tttagacata aatgaagagg gaaatttatt agttcaaaga agtgatggaa
      961 gtataaaaga aattatttca ggtgaagttt ctataagagg agaaaaagga tatgtataat
     1021 aatattttgg atatagaagg cttaaaggtt gggcaggttg aggatagaaa tggtcttact
     1081 gggtgcacag tagtaatatg tgaagaagga gctacttgcg gtgttgatgt aaggggctct
     1141 gctccaggga ctagagaaac cgatttatta gaccctgtaa atatggttca aaaagttcat
     1201 gcagtagttt tggctggagg ttctgcatat ggtcttgaat ccacttgtgg tgtaagtaga
     1261 tacttagaag aaaaaggaat tggttttgat gtaggtgtag gtaaagtgcc tatagttgta
     1321 ggagcagttt tatttgattt aggagtaggg agctttaaat gtagaccaga tttaaagatg
     1381 ggttatagag cttgtgaagt tgcaaatact gatattttaa atcaaggaaa ttatggagca
     1441 ggttgtggag ccactgttgg aaagataaga ggtcaggaat atgctatgaa aggcggaata
     1501 ggtagctatt ctataaaatt agataatggt ctagttgtat ctgcattaat tgctgtaaat
     1561 gctcttggag atgtttatga aaatggtaag gttatagctg gtgctttaaa tgataaaaag
     1621 acagaaacaa ttgatacata taaattaatg aaacaaggtg aaagtaaagg tggatttagt
     1681 gtagataata ctacaattgg gataattgca acaaatgcta agttaaccaa agctggatgt
     1741 aaaaagatat ctcaaatggg acatgatgga tatgctaagt ctatttttcc tatccataca
     1801 cctcatgatg gagatacaat attcacaata tcaacaggag aaatagaagc agatataaca
     1861 ttagtaggtt cattggctgt agaggttgtg gaaaaaagta taataaatgc agtaaaaaaa
     1921 gcggatagtg tagaaaatat aatggcatat aaagatatag taaaaaaacg agaaatatga
     1981 tgaaattagc atatttttct tgtcaaattg ttgacaatgg caactcatct tgataaaatt
     2041 attcttgtta gagcgggcat cctacaatgg tagcttgact cataattata agaaaaaaac
     2101 gaaaaaatta aaaaattaag tagtccggca tcttaatgag ctggaacgga ggtgtatttt
     2161 ttatgatgaa cacaagtgca tcagtaagaa aagtaaatgt aagaaagatg actataatag
     2221 gagtactatc agctatttct attatgctat ctatgactcc attgggattt attccagttg
     2281 gacctacaaa agcaactata atgcacatac cagttattat tggagctatt atggaaggac
     2341 caatagttgg ggcagcaata ggatttatat ttgggatatc aagtctctta aatgctataa
     2401 caaacccaac agtaacatct tttgttttta taaatccatt agtttcaata ttaccaagag
     2461 ttatgatagg tgtattagct tattatgtat atcagttaat aattaaggct acaaataagg
     2521 tatacatatc aggtttaatt acaggggcaa taggttctct gttaaataca gctggagttt
     2581 taggaatgat atatgtctta tatgctgaca agtatcttca tgcaatgggt aaaagtggtt
     2641 cagcaggaaa agtaataatg gctcttgccg cagcaaatgg ggtgccagaa gcaatagtag
     2701 gggctttagt agtagctgct gttgccactg tacttaagaa aagcaaaaaa tagaaagaag
     2761 gttaatgaca tatgcttcta gtatttgatg ttggaaatac taatatggtt ttaggtatat
     2821 ataaaggtga taaattagtt aattactgga gaattaaaac agatagggaa aaaacgtctg
     2881 atgaatatgg aatcctgata agtaacctat ttgattatga taatgtgaat ataagtgata
     2941 ttgatgatgt tataatatca tctgtagttc cgaatgttat gcattctctt gaaaactttt
     3001 gtataaagta ctgtaaaaaa cagccattaa tagtaggtcc aggcataaaa acaggtctaa
     3061 atataaaata tgataatcca aaacaagttg gggcagatag aatagttaat gctgtagcag
     3121 ggatagaaaa gtatggagca ccaagtatac ttgttgattt tggaacagca actacatttt
     3181 gtgctatctc tgaaaaaggt gaatatttgg gtggaacaat agcaccagga ataaaaatat
     3241 ctagtgaggc gttatttcaa agtgcgtcta aattacctag agtagaatta gctaagccag
     3301 gtatgactat ttgtaagagt actgtatcag ccatgcaatc tggaataatt tatggatatg
     3361 ttggtttagt tgacaaaata ataagtatga tgaagaaaga attgaattgt gatgatgtta
     3421 aggttatagc tacaggtgga ttagctaaac tgattgcttc agagacgaaa agtatagatt
     3481 atgtagatgg ttttttaaca ctagaaggat tgagaataat atatgaaaaa aaccaagaat
     3541 aattaatata cagggaattg ggttatatat ccaattccct ttttatgata gatttctagc
     3601 tatatgtggt atattataat gaggaaataa gaatttaaag ttaaatcaca aaataaaggc
     3661 aaaaatagga gaaaaacaat gaaaatagga aatttagaat tgaaaaataa agtttttcta
     3721 tcacctatgg caggtgtaac agaccttcca tttagattaa tttgtaaaga acagggatgt
     3781 gggcttttat atacagaaat gataaatggt aaggcacttt gctatgatga tgaaaatact
     3841 aaaaaaatgc ttaagataga agaggaagaa catcctgtag cagttcagat attcggttca
     3901 gaaccagagt ttatgggaag agcagcagag ataatgaatg attattcaaa tgagattctc
     3961 gatataaata tggggtgtcc agctccaaaa gtagttaaaa atggagatgg ctcagcactt
     4021 atgaaaaatc ctaagcttgc agaagaggta ttgagggcag tagtgaagaa ctcaaaaaag
     4081 ccagtgactt taaagattcg aaaaggctgg gatgataata gtgtaaatgc tgttgaaata
     4141 gcaaagatag cagaggattg tggaataagt gctttagcaa tacatggaag aactagagaa
     4201 cagttttata caggcaaagc agactgggat ataattgcag agattaagaa aaatttgagt
     4261 atacctgtta taggaaatgg tgatgtattt acaatagaag attcaataaa tatgttagac
     4321 aaaactggtt gtgatgctat aatgataggt aggggtgctc aaggtaaccc ttggatattt
     4381 aaaagaataa accattatat gaatacaggc gagattttgc cagagccaac tcttaacgaa
     4441 aaaataagta ctgccattaa gcatttaaaa cttgcagtag aagaacatgg agagtatgta
     4501 gctgtaaggg aaatgagaaa acacatcgct tggtatttaa aaggacttag aaactctgca
     4561 aaattaaggg atgaaattaa caaaatagaa gactatcaag aagttgtgtc gaaattggag
     4621 tactatatgg aagactcctt gacataaaaa taatagtgac ttataatata gcgcataaat
     4681 taaaaaaaga ttataaaatt tttaaaatat aattaggaaa attcctaaga aatttgtgaa
     4741 ggagttgtat ttataatgga agaaaataaa gagtttttat tgacacaaga gggatatgat
     4801 aaattagaag aagaattaga aaacttaaag gttgtaaaga gaaaagaagt tgcagagaga
     4861 ataaaagttg ctatatcttt tggtgattta tctgaaaatg ctgagtatga tgaagctaaa
     4921 aaggaacaag cacaagttga agaaagaatt ttaaaattag aaaatatggt tagaaaagct
     4981 gttatcatag atgaaagcaa aatagacctt aatgttgtta caataggatc aatagttaaa
     5041 gttaaggatt tagaatttga tgaagaggtt gaatatacta tagttggttc gacagaagca
     5101 gacccatatg atggtaaaat atcaaacgaa tctcctgtag ggaaagcact tttaggtaga
     5161 gctgcgaaag aagttgtgga agtgcaagtt cctgatggag ttgctaagtt tgaaatatta
     5221 gaaataagaa gataatttgg aggaaaaaaa tgaaaaataa tcaacaaagc aatgaagaag
     5281 cgcaaattca agaagacctt agtgaagtac ttcaagtaag aagagataaa ctaaaaaaat
     5341 tacaagagtc aggtagagat ccttttaaag aaagtagata tgatagaact cattattcta
     5401 tggatataaa agataacttt gattctcttg aaggtaaaac tacaaaaata gcaggacgta
     5461 taatgagcaa gagaatacaa ggaaaagctg gctttataga tattcaagac caagagggaa
     5521 gaatccaatc ttatgtaaga ctagatgcta taggagagga agaatatagt gtattttcaa
     5581 catatgatat tggagatata gttggaattg aaggagaaat ctttaaaact aaaaaaggtg
     5641 aaatatctgt aaaggctaaa tctgtagtat tactatgtaa atctttacaa gttttaccag
     5701 aaaaatatca tggattaaaa gaccaagaac taagatatag acaaagatat gttgacttaa
     5761 tagtaaatcc agaagtaaaa aatgcttttt taattagaac aaaagcatta aaagcattaa
     5821 gagcatactt agatgataga ggatttttag aagttgagac tccaatatta aatactatag
     5881 caggtggggc aaatgctaga ccatttataa ctaatcataa tactttacat ataccaatgt
     5941 atttaagaat agcgaatgaa ttgtatttaa aaagacttat tgttggtgga tttgataaag
     6001 tttatgaaat gggaagaatg tttagaaatg aaggtatgga tttaaaacat aatcctgaat
     6061 atactgcaat agaattatat caagcatatg cagactatac tgatatgatg gaaataacag
     6121 aaaatgttat tgcacatatg gctgaagttg ctacaggaag tatgatagtt aactatcaag
     6181 gaacagaaat aaactttaca ccaccttgga aaagaatgag tatggaagat tgtgttaaag
     6241 aatattctgg tgtagatttt tctactataa atacagatga agaggcacta gaagttgcta
     6301 gagaaaaagg tatagagata aaacctggaa tgagaagagg agaagtaata aatgctttct
     6361 ttgaagaatt tggagaagat aaattaatcc aaccaacatt tataactcac catccagttg
     6421 aagtatctcc attatctaag agaaatgttg aagacccaag aagaactgat agatttgagg
     6481 catttgctaa taagtgggag ttagctaatg cattttctga acttaatgac cctattgacc
     6541 aaaaaggaag atttatagac caattgagaa aaagagaatt aggtgatgat gaagcttttg
     6601 aaatggatga agatttctta aaagcattag aggtaggtct acctcctaca ggaggactag
     6661 gaataggtat agatagagta attatgttat taactaactc tccatctata agagatgtat
     6721 tattattccc gactatgaaa cctattgata acaatccaaa taaagaagaa gaaaactaag
     6781 aaatattagc attctactaa gttttaaata aagtttagct attaaattat caataaatag
     6841 ctaaatttta tttaaaatgt cagtgtggaa tgctttttat atgcttcaaa gattctttta
     6901 gatatttaat ttataaatta ccaacaaacg ttagaaattg atattagttt ttgttgattg
     6961 agactttata tttagaaaat atgattgaaa gtaatttatt atgaaaatat ttagaataat
     7021 ttatgagaag gtaaaaagaa taatttatga gaatactata tataaaaaat tattggggga
     7081 atgtctgatg tttcaagtaa ttaatttttt atgtttattt tgtgttggat ttttggttct
     7141 tgatgttatg atatattttt gtaaggatgc aatggatatt tttgagggta caactaatgt
     7201 taataaagtt aagagtataa atgtttgggc agatagagtg agagctaatt catattctta
     7261 tgaaaataaa attgcaaaat aggtaaaatt atatatgcat gtattgtttg taatatcaag
     7321 ggttatatct attagtttta attatattta ttaaaaaaac caaaagttat aacctttttt
     7381 aaaataaaaa acaaaaaaag tattgacgaa acatttttaa gatgatatag tattacttgt
     7441 ccttaagaaa agggcaaaca taa
//
LOCUS       DAEOSP010000172         7375 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.176, whole genome shotgun sequence.
ACCESSION   DAEOSP010000172 DAEOSP010000000
VERSION     DAEOSP010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7375
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.176"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(22..684)
                     /locus_tag="KRH54_002914"
                     /pseudo
     CDS             complement(22..684)
                     /locus_tag="KRH54_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(844..1380)
                     /locus_tag="KRH54_002915"
     CDS             complement(844..1380)
                     /locus_tag="KRH54_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG8580552.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(1453..1866)
                     /locus_tag="KRH54_002916"
     CDS             complement(1453..1866)
                     /locus_tag="KRH54_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG8580553.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2359..2997
                     /locus_tag="KRH54_002917"
     CDS             2359..2997
                     /locus_tag="KRH54_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBG8580554.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEGTI
                     SDMQSNINGIEGTISSMQGNINGIENTISGMQGTIGDMQGSISGMNGTISDMQGSISS
                     MDGTINSMQGSITDMQVEIKEVNSRLDNMEPDIKDLKTGQVETSSRLDTIQSDVTEIK
                     SDVKINRNTLDTVIKHTSSLVKDTSDIKNDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(3605..3784)
                     /locus_tag="KRH54_002918"
     CDS             complement(3605..3784)
                     /locus_tag="KRH54_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG8580555.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDKSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(4010..4543)
                     /locus_tag="KRH54_002919"
     CDS             complement(4010..4543)
                     /locus_tag="KRH54_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBG8580556.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIVPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            complement(6086..6658)
                     /locus_tag="KRH54_002920"
     CDS             complement(6086..6658)
                     /locus_tag="KRH54_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG8580557.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(6720..6983)
                     /locus_tag="KRH54_002921"
     CDS             complement(6720..6983)
                     /locus_tag="KRH54_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG8580558.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(6983..7204)
                     /locus_tag="KRH54_002922"
     CDS             complement(6983..7204)
                     /locus_tag="KRH54_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG8580559.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
BASE COUNT         2588 a         1251 c          890 g         2646 t
ORIGIN      
        1 agtgttcgta gctatttttt aatcaaactg gaatacagac tccattccta ggaaattggc
       61 tgaaatatag tctgctgtca aagatgctgt catcacagaa attaaaacag atactgaaaa
      121 gtttttatgg acttcttcaa gtgaaaacat aacacccgct agtggtgcat gaaaggctgc
      181 tgataatcca gcacttgccc cacaggttaa caggaatttt tcctctgtct tccctcggtc
      241 aagagctcta gaaatacctt tacctgccat agcaccaagc tgaatggaag gaccttccct
      301 tcccaaagca agaccaccca tgagtgataa gaatcctcca ataaattttg ttggcaacac
      361 tcgccaccat acttgtttta ttttccctgt catttccccc tcaagctgag ggataccact
      421 tccagaaatc attggctccc agtccaccaa ctttgataca atccacgcaa gtactgcaag
      481 tactgcaaac caagctatta tttttattgt agaaccttct acaaatttta aaatttggct
      541 aagccaagta ccagcctgcc ctaaaagtat cctgtataaa agcacaatca aaccagccac
      601 agccccaacc ataagtcctt caccaatcaa aattacctga aagtgctctg cccgtttgat
      661 aatatgagat gtatcctttt tcatactgtt cctccttcat ccaataattc tacacaatct
      721 gaatctccaa tgactaaagt catgagtgtg tacagatatt ttttaatcaa caaaaataga
      781 tttttattta ctaaatataa gaaaataatt gctcaatttc tcaataaatg tgaatttatt
      841 cttttaggta tttattctcc acagtcgtgc ttcttccagc aaaatattta ttagttcttg
      901 ctttctctgt gtatagattt tacgattgtc ctcaaactgt aatgctaatt tttgtttatg
      961 actatcatag agttttgctt tgtctgtaaa agtatttaga taatctcgaa aattgatata
     1021 attattccat gcaacactat tccattcaac aacatgaata tggtgagtac gagtattttt
     1081 ctcaaaatct cccatagcaa aaagtaattg ttttggtaca tcctgacctc gaaaaacgat
     1141 tccacattgc tctaaccttt cgaggtataa tttagcttcc tctaaagaag caacacctac
     1201 aacaatatca ataataggct ttgcgctaat actgggaata gaagtgcttc caatgtgttg
     1261 aatatcaatg gcagtcttat cccatatact tttcaataat ataatgacat cttttgcact
     1321 tttatcccat atcatctgat gttccaataa tttaaccata ccacatttta aacctatcac
     1381 tatataccac ctcaatataa tccaccttta tatatattac aattttttct atcttgataa
     1441 acagtgattt agctatttca ctttttcttg acactcgcta cattccctat catgaataga
     1501 tataattcct ccacatttag aacaagtata tctctccttc tgcttttgca taaacttctc
     1561 taaaccgtac tgctgaacaa atttactatt ttccataagg cttgcttgat atctcttgtt
     1621 ataacttttt tcaagatttt ttatgagttt acaaggaaaa ttgaaacatt caaaacaata
     1681 aaacaaccct ttttgacaga cacaatcctt tattttacac ttacggcagt gttctggctt
     1741 tcctgtatcg ccattcaaac aaccagcaca tggctttttg tgataacaat gcttatagca
     1801 aaccatacaa ttcattccac aaggagcaaa cataatcgta tcaatactct cttttggcat
     1861 tttcataaca ttttactcct ttcaaaaatc caatttgtcg aattgttata tattcatatt
     1921 gtaacataaa aaatagctaa aaaattcatg cactttcatt gccaataaat tttcaaggaa
     1981 atatgaaagt ttaaaactaa gaataaaaag agataaaaag gaagctacta atcaaaatat
     2041 tcaaatacaa gtaatcaata taaataactt aaggtatatt taaaagtata tgatattacc
     2101 agcagtttta aatacaaata taaccacaca tgcctcaaaa acacttatta cagtatctta
     2161 gaaacttaga caatacataa gattcattaa taaaaaagtt ctcaatatag tcattaataa
     2221 aaacgttctc aatatagata tatttaacca tgttttgaat agcagataat tatatgatta
     2281 aaaattaaat atgacatagc gtttgtggaa tataacacct aaattatgtt ataataataa
     2341 aacaggagtg tgatagtgat ggatgaaaaa atattagagt tattacagga aatgcaaggt
     2401 aatattagta atatgcaagg tagtatcagt ggtatcgaag gcactattag cgatatgcaa
     2461 agcaatatca atggtatcga aggcactatt agcgatatgc aaagcaatat caatggtatc
     2521 gaaggcacta ttagtagtat gcaaggcaat atcaatggta tcgaaaacac tattagtggc
     2581 atgcaaggca ctattggcga tatgcaaggt agtatcagtg gtatgaatgg cactattagc
     2641 gatatgcaag gcagtatcag tagtatggat ggcactatta atagtatgca aggcagtatt
     2701 actgatatgc aagttgaaat taaagaagtc aatagtaggt tagacaatat ggaacctgat
     2761 attaaagact taaaaactgg acaagttgaa actagtagta gacttgatac tattcagagt
     2821 gatgttacag aaattaagtc agatgtaaaa attaacagaa atacattaga tacagtgatt
     2881 aaacatacct cttcattagt aaaagataca tcagatatta aaaatgattt aaataatata
     2941 gagatagtca catctaagaa ttcgaatgat attgctaaat taaaatcagt taaataatat
     3001 actatagtaa aaaatacctg aaaatttcac atacttcttt aaagttatac caaattttat
     3061 acagaaatac tgattatccg acaacacact ttacttataa ataatttatt aaaaaaatac
     3121 tcattttatc ctaaaattaa cacgactaaa tgagtcttga tataataata aaaaataagg
     3181 gagattggac actaaaaagt tgtgtatctc ccttatattc tataatatag ttatataatt
     3241 tttcagccta aaataaaagt ccatctttga ttaaaacaca tccccttatc acttataaaa
     3301 ggataaaatc ttgttttcat ttttccattt atatcttctt gcttaagagt aataattttt
     3361 tcttttgata ctttaagtgt ttggttaaat ttaaccaatc gactaagttt tgttcaatat
     3421 ctatttttca tattttccaa tacttaatct attttttata aataattctt tcatttctaa
     3481 ctcacttaca ttttttacat aaaacgaaac atataaattt caaccttatc ttgtatggtt
     3541 cttaatactt aatgagtagc ttaagtatca atcattataa atttttccct tacatttaac
     3601 actcttacca tttccaaaat atacaaaata tattttgaac atttgtacaa tgatgaaata
     3661 caatacatga aaatacaata tcataactct tgtctaaaaa tagaagttct tcaacataac
     3721 ctttcacaaa aaacatatta tctaagtatt ttttgtctgc tccttgtttt cctgtttgta
     3781 gcatataaat tactgcatct aaacatataa ctactcacac gaaaaaagaa aataaacttc
     3841 caaaatacaa gctctaaaaa ctacctctaa agttgtataa tgtttactag gaataaggtt
     3901 gtataacata tagtttaaat aatcattttt taaaattgca agttattgct ttcaaaatta
     3961 gatgttttta tatcaaataa atgattaaga atttctaaat ttaatggctt tataatgcac
     4021 tccctttctt tgggataaaa taatgttgca cagatacttt ttctaaagat aacaatttta
     4081 tttttctatc aattccacca gcatacccag tcaaactccc atttgttcct acaacacgat
     4141 gacatggaac aataatagag attccattat gccctactgc accaccgatt gcttgtgctg
     4201 acattttaga tattcctttt tgttgagcaa ctttatatgc aatttctcca taagtgattg
     4261 ttttaccata tggtatttgt tgtaaaattt tccacactgt agtctgaaaa ggagtaccta
     4321 ccatatggag tgatggtgta aaattgggtt cttttcccga aaaataaata tctaaccatt
     4381 tctttgtctg ctcgaaaatt ggcaattctt tttcttgatg ttcttggtca agattataag
     4441 cataatactt ttgattctca aaccaaagac cagttaaccc aatattatct gatgctaaaa
     4501 gaatatttcc aattggacta tggtaatagc taatatattg catacaattc ctccttatat
     4561 tctgaaaact tcactcaaat cattagacac ttcttttcta tctaccatta tgtaaaaaca
     4621 tactaatata atttccttct aactacactt tcgttataat atctatcctc atatcatatt
     4681 agttttatat ttacctgcat ctgttcatcc ttttttccct ttccctttga tgaatataaa
     4741 attcatacat ttaagcttcc tactgtatta acttttatag aagtgtatat tataatttta
     4801 tcaccaaata gaccaaaaaa atatcgatat aacatcaagt aatatcataa tactttcaaa
     4861 tgggaaaagt aagaataggc tgtaaaaaag aaaagtattt ttatgttaga aatccagtaa
     4921 aatcgttagt agatagaata acagaaaacg aacaatttgc tatttatcat tttacacata
     4981 aatataattg gcgcaataaa gtgttttcta ttctacaacg attaataagg ttagaaaaaa
     5041 gtaaaaatga actgtatatg tatgaagcat tagtttctgt ttctttcctt tggcttatta
     5101 tgataaaaaa tattgttttg ccaccaataa aacacaaaaa tataattaat atccatatgc
     5161 aaaaaatttt acgttatatt gaaaaatact attgttttac attatattga agggtactat
     5221 tgtgaaaact taattttgga gtactatcaa gcagtggtaa tatcaataaa tcagagtgct
     5281 ccagatgatt tgaactaagt ttaaatacta taccttataa atattttact gagttttgtt
     5341 tattaaaagc aactcaattt ctaaaaggca caaatgagct aataagtgac attgctataa
     5401 aagttgtttt tcatcaaata atctatttta gtaaatgttt taaagaaaaa acgggatatt
     5461 ctccaaaagt gtacagaaat ataaaaaaag agatatgatt ttttatttct tttgattatc
     5521 caaagagtaa ataaaaaata ttgtaaaaaa cttactaaca acggcaatgc tttgaactat
     5581 ggtaatactt tgaacttttg attggacttt ttttgctaaa ataatatatt taaatagcaa
     5641 agtaatctgt ttaatcaata aaaaataaga gataaaagaa atcaactaaa acttttacct
     5701 cttattttga tagtttatca gagctaatat ttacactctt tttatttttg atacttcatt
     5761 aaaaatgatg cttattctct tttcattttt aatacttcat tagaacttat gcttattctc
     5821 ttttcacttt taattcttca ttagaactta tgcttactct ccattcactt ttaattcttc
     5881 attaggacta tgcttattct ccattcactt ttaattcttc attaaaaatg atgcttattc
     5941 tccattcact tttaattctt cattaaaaat gatgcttatt ctccattcac ttttgattct
     6001 tcattaaaaa tgatgcttat tctctgttca cttttaattc tttattaaaa ctgatgctta
     6061 ttctccattc actttcaatt cttcattaaa actgatgctt gcactctgta aactcataaa
     6121 catccattaa tgcttcacca aatctttgat agtgaactac ttccctctca cccaaaaatc
     6181 tcaatatatc tttcaaatca gggtcatcag ttaaatttat taattgatag tacgtagcca
     6241 atgctttttg ttcagcagcc atatcctcat gtaaatcagt taccgaatcg ccaaagacat
     6301 taatataact tgtagtaaag tttactccat tagaatctac tggctgaaga ccatgtccaa
     6361 aattagcata attactacca agtccagcag ccttaagttc ttctggagta caattgccta
     6421 ttaattgata taacactgaa gaaattatct caacatgagc catttcctct gtacctatat
     6481 cagttaaaag tgcacgagat tttccagttg gcatagtata tctttgttga agatatctag
     6541 ttgcagctgc caactcacca ttaggtcctc ccaactgagt catgatatat tttgccattc
     6601 tagggtctgc ttgtcttatg ttaactggat gttccagtgt tttttgataa atccacatat
     6661 tatcttactc ctccttaata tatatttaat aataaatcta attttaaagt ttaataattt
     6721 tatttataaa atgatttgtc ccatggccaa ggttcagata tccatgacca aggatattca
     6781 cttgattcat atccaaaatt agttaatgct ccatatttag attcataatc acacacagct
     6841 tgtgtaaatt tatcgcagta tctgttgtat gaatcaagag ctttcttatc ccttggattg
     6901 ttatctaaat ataaatttaa ttctatacag gcaaattgat attctgaaat tctctctaaa
     6961 agttctcttc tatttaatga cattagtaca tgttaccccc ttttttagct ctaactctat
     7021 ttttaggatt tgcatatagc caatcattat agttatttga ctctattaaa tctaattcag
     7081 gaaataaagt acctcttttt aatgctgttt ttggattgta tgtgtcatca taaatttgac
     7141 gattgatgta tggtctagca aattcacaac aatagtcttg tttatcctct tttaaatatc
     7201 ccattaatta tcctccttaa tttttagaat tttaaaatag cattattttt aaaaattgta
     7261 tactggttct atattataac tggtagtttt aataggtctt taataagatt aaatgattaa
     7321 ataagtatct ttttttctta ttttacctct aaaaattttt ctaattagtc tttca
//
LOCUS       DAEOSP010000173         7261 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.177, whole genome shotgun sequence.
ACCESSION   DAEOSP010000173 DAEOSP010000000
VERSION     DAEOSP010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.177"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            627..1478
                     /locus_tag="KRH54_002923"
     CDS             627..1478
                     /locus_tag="KRH54_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBG8580560.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            1482..1952
                     /locus_tag="KRH54_002924"
     CDS             1482..1952
                     /locus_tag="KRH54_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580561.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            1965..3275
                     /locus_tag="KRH54_002925"
     CDS             1965..3275
                     /locus_tag="KRH54_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8580562.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKKEYLTFLEKHDKTL"
     gene            3487..4233
                     /locus_tag="KRH54_002926"
     CDS             3487..4233
                     /locus_tag="KRH54_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8580563.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTDDSI
                     SLLIDDGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            4595..6136
                     /locus_tag="KRH54_002927"
     CDS             4595..6136
                     /locus_tag="KRH54_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBG8580564.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            6171..>7261
                     /locus_tag="KRH54_002928"
     CDS             6171..>7261
                     /locus_tag="KRH54_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG8580565.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFQGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGEIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVY"
BASE COUNT         2544 a          980 c         1218 g         2519 t
ORIGIN      
        1 ttattatcat ctcatttgtt atcgtcttac ttaatttact tattgctatg tcaattcatt
       61 tattattatc tcacttgtta tcgtcttact taatttactt atttctatat caattcactt
      121 attatcatct taattcattt gcttattatc atttacttta ttcaatttct atatcaaaat
      181 tagtatttgt acttaatact taaaattaat ttaaagtctt tacatattat ttattttcta
      241 ttgtaatatt catttccata ctttactaag ctacttccac tcatttattt taaaatttca
      301 atttactatt tctcaactct atattattat ttttcaattt tatattacta attttaaagc
      361 tttttagcta ttattcctac aatatttaaa caattttttc ataataaagc tatattacaa
      421 tatattcaaa atatgtaata ttttagatat ttttcgttga caatgttaaa tttttgtttt
      481 atccttaatt aaggacatgt tattgacatt tctgaaactt atttgaaata gttttttcat
      541 tttatttagg aaaacgtatt atacttaaga aaacgtattt tctcatttca gataagtaaa
      601 atatttattc ttttggggag gaaatcatgg aaattaataa aaaacatttt attcaagtat
      661 ttttaacttg cttaatcttg gtagtaatat gtgaatttat agggcaaaga actatagcta
      721 ttggaaaagc ctctatagta ctgttcccta tgctttatgc agttataatt ggacttataa
      781 taacaccaga tttattaggt aaaaaaatta aagcacttaa aaaagtggtt ggagaaaaag
      841 aaataaatat tgctggtgaa gttgttggaa ttgcactagt aatgcttggt ataaaatatg
      901 gtactacagt tggaccaaac attgacaaaa taattcaagc tggaccagcg tttatcgctc
      961 aagaatttgg tcatatactt gcaccaatcg ttgcattacc tcttgcatta atgtttggaa
     1021 tgaaaagaga agctgttgga gctactgcaa gtataagtcg tgaaccttct ttaggagtta
     1081 tatcagaaaa atatgggata aactcaccag aaggtagtgg tgtacttggt acatatttaa
     1141 tgggaacagt tcttggtact atatacttta gtgtactagg ttcaatatct atttatagtg
     1201 gtcttcaccc atatgctttg ggaatggcct gtggtgttgg tagtggaagt atgatgacag
     1261 cagctgctgg ttcattatct accatggtac cacctgaaat ggcagataca atacttgcct
     1321 atgctgcaac aagcaatatg atgtctagta taacaggaat tatattctta gtttttgtat
     1381 ccttaccatt tactaatttt atgtataaga ttttagaacc aaaattttcg agaaataaaa
     1441 aaactaaaga aattaaatct acagaaggag agtgctaata aatgtttgct aatttaaatt
     1501 taaagaaaag ttcatatcaa ttattggtta tgtctgttat tggagttata attcttatag
     1561 gacaaagaat tagcgttggt acatctatag ttacagctct tcctggtatg gtgatgctaa
     1621 tattagctgc tatggcagct atgattataa aagatttatt tcctaaatca atctttccag
     1681 cttttggatt tgcaactata atcggccttt tacttagtat gccatatagt ccaacatctg
     1741 aagtattctt aactaacact aataatatta actttatggc tataactact ccactactag
     1801 cttttgcagg aatttctgtt ggaaacaaaa tagaagcttt aaaagaaatg tcatggaaaa
     1861 tagttataat ttcacttatt gtatttacaa ctatattctt tgcatgtgcg agtatagctc
     1921 atatagtttt aagtattcaa ggtaaaatat aggaggaatt tattatgaat aaagaacaat
     1981 taaaatctct agtaataaaa actatagatg aaaatagaga aaaaatatta aaaataggtc
     2041 aaggaatata caaaaatcca gaatatggat ataaagaatt taaaactaca gaagctgttt
     2101 caaacttttt tgagcaagaa ttaaatttat ctgtagaaaa aaatatagca gttacaggat
     2161 gcaaagcaga tgcaaattct agcaagaaag gtcctcatat atctatactt ggagaattgg
     2221 atggtatatc atgtaaagaa cataaagatg ctaatgaaat aggtgcttct catacttgtg
     2281 gacataatat tcaaatagct ggaatgttag gtgctgctgt tggtttagta aaaagtggcg
     2341 ttttagaaca tttagatggt aaagtatctt ttatggctac tcctgctgaa gagtttattg
     2401 agttggaata cagagagcaa ttaaaaaaag aaggaaaaat aacttatttt ggtggaaaac
     2461 aagaattaat aaaaagaggt tattttgatg atatagacat atctatgatg tttcatgctt
     2521 ctgatttagg agaagataag gctctagttg gtcctgagag taatggattt gttggaaaaa
     2581 aggttaaatt tataggtaaa gaatctcatg ctggttctgc tccacatgat ggaataaatg
     2641 ctttaaatgc agcaatgctt gctataaaca acgtgaatgc acttagagaa acttttaaag
     2701 agtctgaaag agttagattc caccctatca tcacaaaagg tggagatata gtaaatgttg
     2761 ttcctgctga tgttcatatg gaatcatatg ttagagctag aactataaat ggtatgattg
     2821 atgctaatga aagaataaat aaggctttga tggctggtgg aatggcagtt ggagctgatg
     2881 ttgagataac tgaaattcct ggatatcttc caatattacg atacaaaagt cttgataatt
     2941 tatttaaaaa caatcttgaa gatttaggtc taaaaggaaa agttattgat ggtggagatt
     3001 ttacaggctc ttttgatttt ggagatgtgt ctcatataat gcctactctt caccctatga
     3061 ttggtggtgt aaaaggagct cttcatacaa gagattttga aatagttgat gaagatttag
     3121 cttacataat tcctgctaaa gcaatggcac ttacagttgt agaccttctt tttgataatg
     3181 ctaaggatgc taaagatata ttagatacct ttactccagt tatgactaaa aaagaatatc
     3241 taacattctt agaaaaacac gataaaactt tataagtcta tttttataat agggtggtat
     3301 ctactgtaaa agttatacca ctctttgttt ttgtaacagt atacttcttt tcttttagtt
     3361 tttagattaa actaaaatat attatcctcc ttaaaacttt tagagctacg ttgaaatatc
     3421 agtagaacta tgctatattc tttctaatta atataaattt ctattctaat tcaaggtggt
     3481 gaaaaaatgt ttaatgcagg aaatcgaatt ataaatacat ggttgtaccc tattcaagat
     3541 ggctttgtct tgattgatac tggatatgaa aatggtttta aacatttcaa aaaacgaatt
     3601 tctaaattca acataaaaat caaagatatt cgatacatat ttctaactca tgctcatgat
     3661 gaccatgttg gttttctaaa tcagattcta gacgaatctc ctaatacaaa gattgttatg
     3721 catgataaag ctttagaaat tctatataaa ggtcaaaact cttttattgg tggttgtacc
     3781 agccgtactg cctttctctt ttgccaaatt atgaaactat ttggaaaagg tgagcatagt
     3841 ttttctccac ttaaacaaga atttgaaaaa aggtgtattc ttgtgtctga caagaatcgt
     3901 aaagaaattg aaaaagaact aaatggaaaa attatagata caccaggaca tactgatgat
     3961 tcaatttcac tactaattga tgatggtgtt ttattttgtg gagattctgc aatgaatgga
     4021 tttccaagta ctcacaaaat taccatattt gcagaaagca aactagaatt tattcaatct
     4081 tggaaaacaa ttatcaatac aaaacctaaa atgatttatc ctggacatgg aaaaccattt
     4141 gaatacagta aattagaaca aaatctaatc tatgtaaata aaataaattt atatcctcta
     4201 cataattcaa aagatattca cagatttttg tagaaaatac aaagtaaatt tctttgatta
     4261 attaaaaaat ctaacacttt actacgctac ttattttaat aacttaatct tccactagct
     4321 tttcataaaa ttttgtttta aaattaattt taaagcgcat atgttattta aatacattag
     4381 atataattcg tcattaatct acttaaagca tatttaacta acaccactca accagaaaat
     4441 tcagaaaact gcattgacac aggaaaaagt ttattttata ctttttttag gacatattgt
     4501 ggacacgtct taaatcgaca agcagaattt aaattgtaag attaaccaca atacttattt
     4561 tttaattata tttttaaaca ttagggagga tattatgaat gataacgtag ttaagaagaa
     4621 aaactttttt gacaggacat taaacagaat agaaactgtt ggtaataaac taccagaccc
     4681 agttacaata tttttagggt tatgtgtact tttacttata ctatctagtc tagttggttc
     4741 tatggggata tcagtagttc atcctggtac tggagaaact ataaccgctg taaatttatt
     4801 gactgtagaa cagcttcaaa ttcttttagg aaacatagtc agcaatttcc aagggtttgc
     4861 tcctttgggt ctagttctag taactatgat aggtgctggt gtatgtgaca aaacaggact
     4921 tatgacagca acaatcaaag catcagtaag caagattcct gaaacaagag taactcttgt
     4981 tgtaatgact ataggtatgc ttgccaatat agcttcagat gcaggtacaa tattatttcc
     5041 accattagct gcacttgtat atcttggtgt tggtagacat ccacttatag gtttattttc
     5101 tggatatgca gcagtctgtc taggatttgc cgcaaacatt atgataagtg tcaatgatat
     5161 acttgctgca agttttacag ttccagcagc tcaaatgatt gattctggat ttcaagccaa
     5221 tgcaacaatg aacttatttt ttatgatagc atcaactttt gttctaatac ttttggcaac
     5281 atgggtaaca gaaaaaattg tagctcctag atttggtaaa tatgaaggaa atgcagaact
     5341 agatgttgat ggaagtctta gtgatgtaga aaagaaaggg cttaaaaaag ctggtataag
     5401 tatattaata tatgtggcta ttatagtagc tcttagtgta atcggtaaaa aaccattctt
     5461 agctgaccca gaaacaggag atttactatc aaacaatgct cctcttatga aaggtatggt
     5521 acctataata gcactagcat ttttcatacc tggtgttgta tacggaaaaa ctataggtaa
     5581 aataaagaag gataaggatg tagtctctat aatggctagt gcaatgagtg atatgggtgg
     5641 atatataata ctagcatttg ctgcttcaca atttttgcaa ttattcacaa atagtaattt
     5701 aggtctgata ctagctgtaa agggcggaga atttttaaaa tcagcaggaa taaatggacc
     5761 tttattgttg ataggattta ttatattatc ctgttttata aatttattta taggtagtgc
     5821 atctgcaaag tgggctattc ttgcacctat atttgtacca atgtttatga tggttggata
     5881 caatccagca cttactcaga tggcttatcg tataggtgat tgtataacaa atccacttag
     5941 cccattattt ccgtacttcc ctattatact agcattcaca agaaaatacg ataaagatgc
     6001 tggtatgggt actgtaattg ctaatatgat accatactct atatcattct taatagtgtg
     6061 gactatacta ctaatattgt ttatggtatt taatattcca ctaggacctg gaatacttcc
     6121 ttcatatagc atgtaattta tttaaatctt tctaaaagga gaaataattt atgttagata
     6181 aaaatagact tataaataat tttatggata tggttagaat tgatagccca tctaatcaag
     6241 aattagaaat gtctaagtgg ttggtaaact acttaaaaga aagaaatata gatgccatta
     6301 tagatgatgc aggtgaaaaa tatggtggta atacaggtaa tgtcattgcc tatataaaag
     6361 gtgaagaagg aagtagacca ctttgtttat gtgcacacat ggaccaagtt caaccttgtt
     6421 taggtgtaaa accaatacta gatggtaatg tagttagaag tgatggaact actacacttg
     6481 gtgcagatga taaagctgga atagctgcta tacttgaagc tttagaacat gttattacag
     6541 aaaaaatacc acatagagat atttatcttt gttttacaat ctgtgaggaa gctggtatgc
     6601 atggtgtcaa aaactttaat cctgacaact tgccttgtaa agatatggtg attttagatt
     6661 ctggtggagc tattggttcg atagcataca aagctcctgc tcaacaatct ataaaaatct
     6721 cttttcaagg aaagaaagca catgctggta tagaacctga aaaagggtta aatgctatat
     6781 tagtagcctc tcatgcaata agcaacatgc acataggcag aatagattca ttgactactt
     6841 caaatatagg tgaaatagaa ggtggtggag ctacaaacat agtaactgat aaggttacat
     6901 tgactgctga aattaggtct catatacctg aaactttaga gtatgagctt aatcatatgg
     6961 aaaaatgttg taaggacgct gcatctaaat ttaatactac ttatactttt gaacataata
     7021 tgtcttaccc tagttttgaa cttagtagag atagtcatgt tttcaagtta agtgaagaag
     7081 caataagaca agttggtgtt gttccaaatc caatggtaat tggtggtgga agtgacgcaa
     7141 atatattagc taatcttgga tataattgtg ctatattaag tctaggtatg tatgatgtac
     7201 acacagttaa tgaatatgta aatatagacg aattatatga tactgccaaa atagtttatc
     7261 a
//
LOCUS       DAEOSP010000174         7255 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.178, whole genome shotgun sequence.
ACCESSION   DAEOSP010000174 DAEOSP010000000
VERSION     DAEOSP010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7255
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.178"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            38..391
                     /locus_tag="KRH54_002929"
     CDS             38..391
                     /locus_tag="KRH54_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBG8580566.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            435..643
                     /locus_tag="KRH54_002930"
                     /pseudo
     CDS             435..643
                     /locus_tag="KRH54_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071281.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
     gene            811..1275
                     /locus_tag="KRH54_002931"
     CDS             811..1275
                     /locus_tag="KRH54_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8580567.1"
                     /translation="MNLGNSLFQARKKHGLSQEDVAEKLGVSRQTVSKWETDETVPDI
                     RQSKKLAILYNVSLDELIDFDIDMEEIQEAINKTSEETEEKINWTNAWGKKYPILLKY
                     QTDVNISNYAYKVNMMLDELKQEYQYNELEAMLILKDILYNVWKNRKSKRKQ"
     gene            1301..1660
                     /locus_tag="KRH54_002932"
     CDS             1301..1660
                     /locus_tag="KRH54_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580568.1"
                     /translation="MDLFEVNIVLNSGTAGGMNPKLKIFNMVISTEVCHHDVTQDTLT
                     EFHSWLKTVFFEANCELIKISEMAVKKTNQEETVFWGRILAGEAFITNKGRKKINEKE
                     AYLHLIASSHIFYTIIK"
     gene            complement(1711..2652)
                     /locus_tag="KRH54_002933"
     CDS             complement(1711..2652)
                     /locus_tag="KRH54_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBG8580569.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(2753..3988)
                     /locus_tag="KRH54_002934"
     CDS             complement(2753..3988)
                     /locus_tag="KRH54_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBG8580570.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYISLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            complement(4032..4886)
                     /locus_tag="KRH54_002935"
     CDS             complement(4032..4886)
                     /locus_tag="KRH54_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580571.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            complement(5014..6189)
                     /locus_tag="KRH54_002936"
     CDS             complement(5014..6189)
                     /locus_tag="KRH54_002936"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBG8580572.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            complement(6288..7172)
                     /locus_tag="KRH54_002937"
     CDS             complement(6288..7172)
                     /locus_tag="KRH54_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBG8580573.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNNFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPYIVHKEEFEHPLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
BASE COUNT         2477 a         1251 c          838 g         2689 t
ORIGIN      
        1 tattacatat caattataaa gagaggtatc taaaatcatg gatagaaaac gtgaattaaa
       61 acaattatac aaagaaatga aatttgacac aggtgtattc ataataaaaa atgatattac
      121 taaaaagatt taccttggaa aaagtaatga tataaaaagc aaatttaact ctctcaaatt
      181 tcagcttggt gctggctcat gtatgataaa agaattaaat aatgaatgga aaaaatatgg
      241 tgaaaaagca tttacatttg aagttttaga gcttataaaa catgatgata acaaaacaga
      301 gaaagactat ttagaagaac tagatatttt agaaatggtt tggttagaaa aattaaagga
      361 agataatact tatgaaattt attccatata aaaaataata taatataaga aaaggagata
      421 gtgtctcaaa atagatattt agttcttgca gttgggaatt tggctggaat atcaaattta
      481 aaaaacctag aaaaaattta tgcacagtat atccgaaaga aaattatttt acgttttagt
      541 tgttgtttga aagaaagaga aagaggttgt cgaagcgatt ttacaggagt ttacaccaga
      601 attaagagat atttacgtac aaacaaaaac agggaatgga taaaagtggt taatgattga
      661 tattcgcaga ggttagcaga ttttaatttg cagaataaat acgtctttga attatgttaa
      721 taaaaatagc aaatatgata attttagttg gtagagtttg ttcttatcaa ataatataat
      781 aatcccataa acagataagg aggaatttca atgaatttag gaaatagttt atttcaggca
      841 aggaagaaac atgggctttc acaggaagat gtagctgaaa aactgggggt aagcaggcag
      901 actgtttcaa aatgggaaac ggatgaaaca gtcccagaca ttcgtcagtc aaaaaaatta
      961 gcaatacttt acaatgtgtc attagatgaa ttgattgatt ttgatattga catggaagaa
     1021 attcaggaag cgattaataa aacaagtgag gaaaccgaag aaaaaatcaa ttggacaaat
     1081 gcctggggaa aaaaataccc tattcttctt aaatatcaga cagatgttaa tatttctaat
     1141 tatgcttata aggttaacat gatgttggac gaattaaaac aggaatacca atataatgaa
     1201 ctggaagcaa tgttaattct taaagatatt ttatacaatg tctggaaaaa tcgaaaaagt
     1261 aaacgaaagc aataaatccc cgttttttgg aaactaaagt attgaccttt ttgaagtaaa
     1321 tatcgttctc aattcaggga cagcaggtgg aatgaaccca aaattgaaaa tatttaatat
     1381 ggtaatatct acggaagttt gtcatcatga tgttacacaa gatactctga cagaatttca
     1441 ctcatggttg aaaaccgtat tttttgaagc aaactgtgaa ttgataaaaa tatctgaaat
     1501 ggctgtaaaa aaaaccaacc aagaagaaac cgtattttgg ggaagaattt tagcagggga
     1561 agcttttatt accaataagg gacgcaaaaa aataaatgaa aaagaggctt atctccattt
     1621 gatagcctct tcccatatat tttataccat tataaaatga acaatctact aattttcaat
     1681 gtgtatattc attttactaa taacactcca ttacttcttt acagcatcca aacattgctc
     1741 tataatactc ttatcactta tacctattgc tgttacttta ctagctccac ttaatatact
     1801 cttatcactt ttatttgaaa ctaaaacaac aggagcattt tttgcaagtg gtgatgcaac
     1861 taaagcataa actaaatcgt ctccacttgt tatatagaac tcttttgctc cattatagaa
     1921 cttttcaata acatttttat tagtttgata tctatcaaca ccgccaattc tagttgctct
     1981 atatctgctc actatgtaat cacttaatgt agactctcct ccaatagcat atactggaac
     2041 aggagaaact ccaaaccatg ggtctaagtc tgtgtcttct tctgctgaag gtatatcttt
     2101 tgtcaatatt ataggagctc catctctata agcaacagat gcaacactta ctgcatcagc
     2161 ttcatcctta aatccattta ctaaaaacat cttatcactg tttttaattt tttctatttc
     2221 gcctgcaatt ttataacttg tatctactct atctacacca tcaattctta taacttgcat
     2281 tcttctcata agtaacattt tttctacatc tttacttata gaattttctc cacctattac
     2341 atatatctta gtaactctat tcagtctttg aagcgttgct tctggtatat catccttatt
     2401 agttaataaa atagctgcat tatttactcc tgcaagacca cttgcagcaa ttccatctac
     2461 cattgtagaa tctgcattga taagaactgc tgttgtaaaa tcttgcttat ctgaaataag
     2521 cccagctgtt tcatatttat ctactccttg aatattttca atcttatcta gtgcgtttat
     2581 tgatgttgga aaactaacac ttaacagtaa tgctgaaata cctaacgcca ataccttttt
     2641 actaatcctc ataaattacc tccctaaaac aaatatattt agtattagta taatataaaa
     2701 tcatttaaaa atagtgtaaa aatagttaca cttttttata atttaaataa ttttagacta
     2761 aacacaattc atccattaaa ataacttcat ttattctata tgctaagcca tcccacgttt
     2821 tttttgacat atctacagat gttttcttat ttgagttatt caataaagat tgtatcttag
     2881 aatctattgc attatacaaa ttattttcaa aatcatataa ctcttcatct tttggaatac
     2941 cttctttctc cataaaaggt agagaaatat attctatctt tccagaatta ttaatctcac
     3001 taccaatcca ctctttaaca ccaagaatat cagtagtgat tacatctgtg ccacaagata
     3061 aagcttctaa aactacaact ggtaatccct cataaaatga tggtaacaca aatatttgtg
     3121 acctgttgaa tagctcagct aaatctctct gttctaactt tcctaggaaa ttcattttaa
     3181 atggagactt acttgccagt ttaactatct cattatatga ttcgatatcg cttccagtac
     3241 ctgcaaaatt gacttctatt aaatctttac tatattttaa tttagatata gcttttataa
     3301 atggaatcag tccctttgat ttacatatct tacctgcaaa tactattttt attttatcat
     3361 cctttatttt ataattttta ttataaaata tatcatcatt ataaccacta ccaattacaa
     3421 ctactttact ttcacttact ccaaaagtct ttataatatc atatttctga ttttcatgaa
     3481 gagcaaatat taaatctaat ttccttatat ttgctattat gtattctttc tctaaatcaa
     3541 tggtatttaa ctgtcttaaa caagttccat gacaaataga cataaccttt atatctttta
     3601 ccatttccct aacaaatgca gttaagaggt acaaatgatg acatattata agttctggtt
     3661 taaaatcatt catagcctta tctatattta ctttaaattg atatttcaat ctatttatca
     3721 tgtctatatt taaatcttta tatcttgtac tttcatatgg catagaatca ctcataccta
     3781 caacaggaaa atttaactcc ccacaattat actttactgg ataaaaactt acttcacttg
     3841 gaaaacaatt tacatcatca tttacatcta tacctgcaat tacagcttgc ttatgaccga
     3901 ttttttcgaa cccttttatc ataccagaca aatatatacc actacctgta ctatttggtt
     3961 tttgtgttat tatatgaaga attttcactt ttcacttgcc tcctttctaa aattaaatag
     4021 ttttatacag tctaatcatt atatatatct aaatataaac aagatgaatc atcttttact
     4081 ttaaatcttg gcactttaac cgctttatag tcattatctt caaaatccct tactttatta
     4141 tgtatatatc ctataccaaa tttttctgct atacgtatca attcttcttc ttttattaga
     4201 ttatatctat cacatgcact tgtaaaacca tcactcgtta gcatcaattg aaaatctttt
     4261 tttatttcaa tgtatccata tatagaattt tccacagctt cctcatcaaa atccaatatc
     4321 caatagccct cttttgtatt tttcttcaat ctattgctta ctattgtatc cataacacta
     4381 gttctaatct catcaaaagc catctcttcc aagttagaaa gtttactcat ttcatcaaat
     4441 acagtattat caaatttaca aatactccta tcttttataa ctttggaatc tccattttta
     4501 ataaacaaag cacaatctcc taataaaaaa tactctactt tattttcatg aaattttaca
     4561 acaactatag aactagatgg taaatctaat ttttcaacct taaacccatt taatcttagt
     4621 tcatactctt tttttacttc tattattcca gtattaataa tactctttaa tgattcttct
     4681 ttatttatat ttttatataa atacctattc caccactgaa cataccacct tgcgtcactt
     4741 tcagttgaca ctaaattttt tccattaagt cctgtagcac catctaaaac ccatgcccca
     4801 aatggattta taccgactac atcttcatta aatttagctg ttccttgatt acataatagg
     4861 ctatagtttc ttatatttaa agccatatca acctccaaat tgaatatcta aaaataaagt
     4921 tctatttaaa attatacagc aaataaaaaa atctacaaca aaacttgttg tagattttta
     4981 cctaatctat attatcttta tactttcagt attttaatga tggaataatc tatttttagt
     5041 aaatgccatg actatgttat acttattaca agttaatata acattatcat cacgaataga
     5101 accacctggt tgtgctataa aacttacccc acttcttttt gcacgttcta tattatctcc
     5161 aaatgggaaa aatgcatctg aaccaagtgc tactcctgtt aaagttttaa gccatgctct
     5221 ttttccttct ccagttagag gctctggttt ttcttcaaaa aagttttgcc atatgccatc
     5281 agcaagcaca tccatataat catcagaaag atatacatca atagtgttat ctctatctgg
     5341 acgacctata tcctttttaa attttaagtt taatacttta ggatgttgac gaagatacca
     5401 agtatcagct ttgttacctg caagtctagt acaatgaata cgtgattgtt gacctgcacc
     5461 tacccctatt acttgtccat ctttagcata acaaacagaa tttgattgtg tatattttaa
     5521 tgtaataaga gctataatta aatcacgttt tgcactatct gtaaaaattt tattatcagt
     5581 tggaatatct tttaataggt cttcatttat taatatttca tttctacctt gttcaaaagt
     5641 aattccataa acatcctttg tttcaatagg ttcaggagta taattttcat ctatttttac
     5701 aatattatag ttgcctttac gctttccttt taatatttca agggcttcat ctgtatatcc
     5761 tggagctatt ataccatcag aaacctctct tgctataata gttgcagtct ccttatcaca
     5821 tacatctgaa agtgctataa aatcgccata agaagacatt ctatctgctc ctcttgccat
     5881 tgcataagca caagctaatg gagaaagctc taaatcatca acaaaatata tttgctttaa
     5941 agtatctgat aaaggtaccc ctactgctgc tcctgctgga cttacatgtt taaatgaagt
     6001 tgctgctggg agacctgtag cttcttttaa ttccttcaca agttgccaac tattaaatgc
     6061 atctaaaaaa tttatatatc caggttttcc atttaatact tctataggaa gttctccatt
     6121 cttcatataa atctttgatg gtttttgatt tggattacag ccatacttta attctagttc
     6181 tcttgccata tactcatccc tctctataat aaattaatca taagtttgat taatcctata
     6241 ttaattaaat tattcgtact aataaaatta tatggcatat ttatccatta atcaactata
     6301 ttttttaaaa ctcctattcc ttcgacttta cattcaacta tatcgccatg ctttaaatat
     6361 acttgaggat tcatccccat agcaacccct gctggagttc cagttgaaat tatatctcct
     6421 ggctttagag tcattcctct tgataaatca tttattatag taggtatatc aaatataaag
     6481 tactctgtat tagaattttg tcttacttct ccatttacta cactacttat atctaatttt
     6541 aaaggatgtt caaattcttc tttatgtact atataaggtc ccatagatgt atgtgtgtct
     6601 aagctttttc ctctaaacca ttgaacatgt ttatcttgaa gtgctctttc acttatatca
     6661 tttactattg tatatccaaa tatatattca taagcatctt cttttgatat atttatacct
     6721 tctttaccta tgattactgc aagttctgat tcatagtcca acttctctac caaatctcca
     6781 tgtaagctta tcttatcatc cactccaatt attttgtctg ctctcttaga aaaataaatt
     6841 ggataatctg gcataactac gttttttata actccatctg ggatttcatt gacatggtct
     6901 ttatagttta gacctaaaca tataatatct tttttaggtc ttacaattgg ggaacataat
     6961 ttaacttctt ctaacttata acactttgcg ttttcaaatg aattgttttt tattttttct
     7021 aaatctcctg tagatacgtt ttcaataaaa ttattcatat cagtatataa tttacttaat
     7081 cctaaactat taatttcata tatactattt gtttctgaat taaatacacc tattttttct
     7141 tcattgctac tacaaaaagt cacaaatttc atttttattc ctccattcaa taagttgatt
     7201 aatattattt aatttttatt agtttatata aattgtaatt cataatattt atatg
//
LOCUS       DAEOSP010000175         7239 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.179, whole genome shotgun sequence.
ACCESSION   DAEOSP010000175 DAEOSP010000000
VERSION     DAEOSP010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.179"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            96..653
                     /locus_tag="KRH54_002938"
     CDS             96..653
                     /locus_tag="KRH54_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8580574.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSKKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(892..1752)
                     /locus_tag="KRH54_002939"
     CDS             complement(892..1752)
                     /locus_tag="KRH54_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG8580575.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            2250..3653
                     /locus_tag="KRH54_002940"
     CDS             2250..3653
                     /locus_tag="KRH54_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8580576.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHKSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESKIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGYKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            3872..6349
                     /locus_tag="KRH54_002941"
     CDS             3872..6349
                     /locus_tag="KRH54_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG8580577.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNTIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSDK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            6364..7050
                     /locus_tag="KRH54_002942"
     CDS             6364..7050
                     /locus_tag="KRH54_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580578.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKDVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TIIVSDGLIV"
BASE COUNT         2772 a          813 c         1079 g         2575 t
ORIGIN      
        1 taaaaataag gttaatttga gaacatttat ataattaata tcgtatttta aatatgaaca
       61 gtatatttag attaatgtat aaagggggat tagaaatgtc tgattctgaa tatcctcaaa
      121 tgaaaacctt agaaactaga agttgtatac taagacctgc atcattagat gatgttgaag
      181 atttttttga ctgttataaa aataaaatag tagtaaaaca tttaccattt agtgagcata
      241 aatctttaga agatacaaga aagtttataa aatcattttt tctaaatcca tataaaaaag
      301 ggaaaattgg ccactttgct atagtatata aaagagacaa taaagttatt gggaatatgg
      361 ggtttaataa tataaatcct aaagcattag aagcagaaat aggtatatgt ataaatccga
      421 gctattgggg acatgatttt gctactgaga taacaaaaag agtaatacag tatggattta
      481 gagaactaaa cttaaataaa ataattgcaa taacctatga agaaaaccaa aattctaaaa
      541 aatcattaga tttattagga tttaaatgta caggaaagta tatcaaaaaa attcatactg
      601 gaaatactgt aaaaaatgtt ccttgttatc aatatgaatt gaaaaaacaa tagatataat
      661 catttaatat ctatgctggt tatttagtaa tgattgtttt ctaaataaaa tttcaataaa
      721 gtggttgcct caaaatatag ataatcctgt ttttattgtt aaaatactga aaatgcattt
      781 gattttatac aaaaaagagt gtctcatgaa ctaaaaagtt tatgagacac tctttttgaa
      841 taaatcgtat tttaattaaa atagtatata tacttaaaat attaattttt attagttctc
      901 taaattttta aatccttctc caagaacctc atgtacatct gtaacaataa caaatgcagt
      961 agggtcagtt cttttaacta atctctttag ataaagttct tgttttttag aaactacgac
     1021 taatagaact tctttatttt ctctagtgta tccaccttta ccatcaagaa tagtaagacc
     1081 tctatcaagt tcttcagtta tagcttgtct taaactttca ggttcatcag agataataaa
     1141 aaatgctttt gaatggtcaa atccttccat tatcatatct gctattttta ttaatacaca
     1201 aagtgctata ccagagtaaa gagcagtttc taaactacga tttactatac cagaagatat
     1261 aacaaccatt ccatctaggc atgacatgaa ttttgtagct tttattcctg ggaatttctt
     1321 actaagtatg agtgcaatta agtctgttcc tccagtagaa gcatctgatt taaacattat
     1381 tcctaaacct ataccaacta ggattcctcc tgcaatagaa gacaataata agtcattagt
     1441 aaatcttagt tttgataatg ggtctgttaa ttgaactata gctgaaaata caactgttcc
     1501 aaataaagtt tttaatgaat tttttgtacc cataattcta aaagcaagta ttactaatgg
     1561 gacacctata attaacatga tagctgatac tggaagtcct gtaaccttgt ttagtactaa
     1621 tgatagacca ctaagtcctc caggagcaat tgtatgtggt tttaaaaaca tatttatacc
     1681 aatactcatt gatatacaac caaaaaatag tccaagcatt tcaagtaaaa ttggcgactt
     1741 ttgttttttc atataaacac ctccatgaac ttgtcacaat ttaatattat aacatatttg
     1801 accagtttat attatataaa atttcttgat ataagcaatt tcaagtattt cattattctt
     1861 aatatttttg aatttttata tagtgttttc atatttttac tgatgttgtt acactcctat
     1921 gaagttttta tatattataa tttttttatt cttttataaa gtatccccta taaagatggt
     1981 tctatgaaaa ttttctctat actgtttaac gtatatagag attttgtgta taattataag
     2041 gtatttaatg gaaaaattta tcttactttt atatgttatc aaaatgattt attaaataca
     2101 acagacagct tgataacata taattatata aatataattt atgtagatag ttgtattttt
     2161 aaataaattt ttacatatca ataattattg tctattttat ataagcaaca atcaaactaa
     2221 aataaaagtt taagaaaaga gttgattaaa tgcataaacc tagttataaa acagtatttc
     2281 attcatacat tatttttttc atgattttta tttgcttaat cctgacagta ataggtattt
     2341 tcttatcact tattactatt cagaaaccaa atggtgaatt tattcgtagt gattggccga
     2401 aaaaatttac agaaaacttt aatgaacaaa tcgtatttat agataataag ccacaagtaa
     2461 aacagtctgg attagaaaaa cttcgagaaa atcaattgtg gttgcaaatt ttagattcta
     2521 gtggaaatag agtttatggg tttttagaac caaaagagca taaagattat tattctaatt
     2581 ctgaattatt agcattagct aaccaaaata attcaagtaa tggaactgta tactttaata
     2641 caattaaaaa taataaaaat gactatattt atattatata ttttccatta aatatatcta
     2701 aagtgaatat gttttttgat ggaggtaaat ttataggtgg taagtcaatt gtattttcaa
     2761 tcatagggat tatattttta ataattttgg tttctggaat tgcttatggt tactgggtaa
     2821 caaagtgcat atcaaatatt acatctgctg ttagcgatat ttcaaaacga agctatcttc
     2881 cagttaatat taaaggtgtt tttgctgata tatataatag tttaaatatt ttaaatgaag
     2941 aaattcataa aagtgatgag atacagaata aaactgatat tatgagagaa gagtggattt
     3001 caaatataac tcatgattta aaaactccac tttcaccaat aaaagggtat gcagagcttc
     3061 ttacagatag tagtactaat tattcacctg aaaaagtata taaatatgct tcaatcatgt
     3121 taaagaacat agaatataca aatacactta taaacgattt aaaattaacc tatcaattaa
     3181 aaaatggaat aatcccttta aataaaaata attatgattt agtaagattt ttgaaagaat
     3241 tatccattga tattttaaat tatccagaat atgaatcaaa aatagttgaa tttaagagta
     3301 atatagataa tattaacttt gattttgatt gtacactatt aaaacgagct tttagtaatc
     3361 ttattgtcaa tgcttttaca tatggatata aaaatactaa agtttgtgta catataacat
     3421 taaatgaaag aattgaaatc attatatctg ataatggaaa agggatgtca agagaagaag
     3481 catcaaattt gtttaatcgt tattatagag gaagcaatac aaaacaaaaa atagaaggaa
     3541 ctggtttagg actagcaata acgaagcaaa ttattgaagt tcatggaggg aatatatctg
     3601 ttgaaagtat acctggatta ggtagctcat tttatatcac ctttcctatt taattaaggt
     3661 taattaaggt caaataaaaa atatattaag gttgatagat tattatattt atatagtagt
     3721 ttatcaatct tttttattta aggggtgatt tgattaaaaa tattataaat ataatgataa
     3781 aaatatggag gagtctatta gagatttaat ttagattaaa gtataaaaat attagattga
     3841 aatatcttag ataagggaag aaggtgtaag tttgaaaatt atattaaagt atattttcac
     3901 aaatattaaa gaacgaaaaa ttagaacagc agttatgtta ttgtccatag ttttatcaac
     3961 agttttactt tttgtttcat tctcaattgg attatcttat gaaagtgcac aaagaaaaat
     4021 ggcaaaaggt atgtatggta cagcgactat atctgtccaa agtaaaaatc cagatatatt
     4081 aaccaattta gaagacatac ctgatttaaa tactataaaa tcaaaggttg gtgtattgga
     4141 aagctctgct atttataata aagggggata ctatgaagaa tttagtctta tatcagcaga
     4201 cttaagtcaa ctaaataaaa tcaacaagcc acgattagaa aatggcgata gtattactga
     4261 tttttctggt gataaaatta ttcttccaaa tcgctttaca tcaaaatata aaataaaaaa
     4321 aggtgattct attactttac agatttatgg aaagtcctat acttttcaag ttagtgatat
     4381 tgcatcttat gatacagtat ttttgaggaa cacaagaggt gtaaatgcac tgctacccaa
     4441 agagactcta tcaaaaatta taaataaagg cagtggatat acaagagttc ttatagagtc
     4501 agaagaagaa attacagaaa atctagtaaa taaattatcc gaagaacttt ctactgaaaa
     4561 atatatggta tcaaatacta taaatgaaac taagataatt tcagatgcta gacaaaagac
     4621 aatgcctttc tttttaataa gtttttttgc attaaccctt agtatattta taatatacag
     4681 cagttataag gttattacat tagaacgtct tccttttatt ggaacattta gaagtattgg
     4741 tgccaatgaa aaaactgtaa ctcgtatatt gatgcttgaa agtatattat acggaagtat
     4801 aggaggatta attgctattc ctattggtgt agttgtttta aatttaatgc ttcatgggtt
     4861 aggaaattct ctcgaacaag gaatatcaat tccagtagta atatcaccca ttggagtgat
     4921 tatatctgtt atagtagcaa taatcgtttc ttcatttagt gcatatatac ctgttaaaaa
     4981 agctagccat ttgccgataa aaaatattgt actaggaaca gtagaagaaa agaatgtttc
     5041 aaatcgttct atacttttta taggaagtat aatgtttatt ttatctattt tacttccaag
     5101 aatatcccct gaaaatacat tgtatctagc tggtggattt tcattactgg gattaattgt
     5161 agctactatt gtgttgattc ctcttattac tgatatcatg tctatagttt ttgagtttgt
     5221 ttataaaaat attttaggga atgagggtaa attagctgct agaaatatga aaaataacaa
     5281 aaatataata cagaatatca ctttactatt tataagtatt tctgcagtta ttgcaataag
     5341 tgttgttggt aactttgtca aaacttatat aacagatgtg tttcgtgacg cagaattaca
     5401 aggttttgca gatggaaaga tgaatgaaga gtttattgaa gatgttagac atatggatgg
     5461 aattaaaaaa atattacctc tttatgttat gaataatgag atttctggta atggtgtaac
     5521 attatcaaga ctagagggta cagataatat aaaactatat aactctatgt ttggaatcaa
     5581 ttatacaaat tttgagataa aaaaacaagt aatagaagct tttaatgata aacgttctgt
     5641 tattttgaat gaagatactc taaaaaaagt aggcttatct ataggagata caataactct
     5701 atctaatgat aagtatgatt tttcttataa aatagtaggt agttttaaat cccgtgcaaa
     5761 tgatgtagaa gctgttatac catcacatta tgcagttaat gattttgata aaactaatta
     5821 tggtttttta gtatatacag cagtcaatcc agatgctgtt atgattcaga taagatattt
     5881 atttggagat acatataatt ggagtcgtac agttgaagaa tttaataatg attctttaaa
     5941 tactataagt agttttttgt ctcctatgaa taagatgacc tatttcattt ttcttcttgc
     6001 tacagtaggt attattaata atctactgat taattatatt caaaaaagac gttctatagc
     6061 tatgtataaa tctataggac taagtgataa acaaaatata aaagtaacat tgattgaagg
     6121 gtttacttca ggtttacttg gagctgttat tggtattgtg atatctatac ttgagattca
     6181 aactatcttt atagttgcag gaccaaaaat ttcaatgaaa ccagatttag attttaaaac
     6241 atttattatt gttgggttat tgggaattat agtaacttta ataggttcga tagtgcctat
     6301 tataaaaggg aagaaaatga aactgataga agaaattaag tttgaataat tatggaggtt
     6361 aatatgaaaa ttacacataa tagtttagct gtagaagcaa aaaatattat caaagaatat
     6421 aaaattggaa atacaacaac tcgagtgtta aaagatgtat ctttacaagt aatgaaaggt
     6481 gaatttgttt ctatcatggg tcaatctggt tctggcaaaa gtactctttt atatatttta
     6541 ggtggtttag atactccaac gagcggaaaa gtgtatatga atggagctga tatatcacat
     6601 tttaatgatg agaaaatgag cataatacgt cgtagaaata taggattcgt atttcaattt
     6661 tataacttaa taccaaattt aaatgtggag gaaaatataa tgcttccttt gttattagat
     6721 ggtaaaaatt taaaagatta taaaaatcaa ttagatgaaa tcttagatat agttggttta
     6781 actgatagac gtaaacatac accacgagag ttatctggtg gtcagcaaca acgtgttgct
     6841 atagctcgag ctttaattgg aaaaccagag attttatttg ctgatgagcc tactggaaat
     6901 ttagatagta aaacagggat agaaataata gatttactga ataaaataaa cagagacaat
     6961 ggacaaacta taattatggt aactcactca ccagaagcag ccaaaagtag tagtcgtact
     7021 attatagttt cagatggttt aattgtataa ggactaatta ttgtatttga gataagcatt
     7081 tttaattata taaattagac atatgtaaag tggatataag ttaaatacta taagtgtgct
     7141 ataatattaa aataacagat aaaagtacta tattttttat tgattatttt aattttattt
     7201 gtttattaaa tatctaatga gtattcatac tatctctat
//
LOCUS       DAEOSP010000176         7202 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.180, whole genome shotgun sequence.
ACCESSION   DAEOSP010000176 DAEOSP010000000
VERSION     DAEOSP010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.180"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            37..729
                     /locus_tag="KRH54_002943"
     CDS             37..729
                     /locus_tag="KRH54_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8580579.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            719..1900
                     /locus_tag="KRH54_002944"
     CDS             719..1900
                     /locus_tag="KRH54_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG8580580.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            2182..2637
                     /locus_tag="KRH54_002945"
     CDS             2182..2637
                     /locus_tag="KRH54_002945"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBG8580581.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            2627..3352
                     /locus_tag="KRH54_002946"
     CDS             2627..3352
                     /locus_tag="KRH54_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBG8580582.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            3324..5705
                     /locus_tag="KRH54_002947"
     CDS             3324..5705
                     /locus_tag="KRH54_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBG8580583.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(5910..7139)
                     /locus_tag="KRH54_002948"
     CDS             complement(5910..7139)
                     /locus_tag="KRH54_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBG8580584.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
BASE COUNT         2831 a          810 c         1275 g         2286 t
ORIGIN      
        1 aagctatata caaagattaa gttaggatgt gtaaaaatga ataatacagt tcttgttgtg
       61 gatgatgaaa aggaaataag agaccttata gagatatatc ttttaaatag tggatataat
      121 gtaattaaag ctgaagatgg aaaagaggct ctagatattt taaaaaagga aaatattcat
      181 ttaatgatat tggatataat gatgcctaaa atggatggga tagaagtttg tagaaaagtt
      241 agagaaagtt tgaatatacc aatacttatg ttaagtgcaa aatcagaaga catggataaa
      301 atacaaggga taatgactgg tgcagatgac tatttgacaa aaccatttaa cccacttgaa
      361 cttactgtga gagtgaagtc gctgattaga agagcatatt attttagtgg tgctaataat
      421 gaagataaag atgtcataaa ggttggagta gtaattatag ataaggaaaa acatagtgta
      481 acagtggctg gaaatgaaat aattttgaca tcaagagaat ttgatatatt atatttatta
      541 gcaaataata aaggcagagt atatagtaca gaagaaatat ttgaaaaggt atggaaagag
      601 aaatattacc aatctaataa tactgtaatg gttcatatga gcagaataag ggataaaatt
      661 gaaaaatata cagatggaga aaaaataatt catactgtat ggggagtagg atataaaatt
      721 gaaaaataaa agtttattaa atcatatgtt tcgacccata ttagatctta taataagctg
      781 tgctttaact tcaatgctta ttttaacaag tatgtatata ataaaatcat tatatgtcaa
      841 tagtgatatg tctgtagtta atcaacttgt atatcaagca agagatttca gaagtcttat
      901 agggtatgat ttattttatc catttttatt tacaatcaca tcattagcaa tttattccat
      961 gatatcgtac aagagaaata agcaaattgt agattttata agaaagacta atagaaaaaa
     1021 tcaaaaatta gaaatcatta aaagtgctgt aaatttaatg gatgaaggta acttagaaaa
     1081 aagtatagat attgagtatg acttagatat attaaaggat aaagaaaagg atgatattga
     1141 tgaactagca cataaaatca ataatatagt ccatcaactt agaaatataa ctattgaaga
     1201 gagacaagct cagcagacaa aaacagattt aataacaaat gtgtcacatg acttaagaac
     1261 acctttgaca tccataatgg gttatttagg agtgatagaa gaaggtaagt ataaagatga
     1321 agttcagatg atgtattatg ttgatattgc atatgaaaaa tcgaaaaatt taaatgttct
     1381 tataaatgat ttgtttgaat tgactaaaat gcaaaataat acaataaaat taaacaaaat
     1441 agaaataaat ttagtagagt tattaagtca agtagtatct caatttgaaa tttattttaa
     1501 acaagaattt atggttagta gaattaattt taatgaagaa aaattaattg taaaagctga
     1561 tccaaataaa ttggtaagag catttgaaaa tcttataact aatgctgtga aatatggaaa
     1621 agatgggcat tatgtagata tagtcacaga gaaaaaggaa gatatggcgg ttgtaaaagt
     1681 gataaattat ggggaaccaa ttccagtgtt agatttacca aatatatttg atagatttta
     1741 tagagttgaa aaatcgagaa atagaaatgt tggaggttct ggattgggtc ttgcgattac
     1801 aaaaaatatt gtaaatttac atgatggaga aattactgtt tcaagcgata aatatcaaac
     1861 tgtgtttgaa gtacaactgc caataataca atcagcttag ttataaaata aaagtacgat
     1921 tatgtgatat aaatattata acaataaagt gtttacaaaa ataaaaaagt aatatataat
     1981 ttaagtcagt ttaataagat acttatcaag agttagggga gagagttagc tcagtgatcc
     2041 tataccaacc ttttatcagg tggtcctgct aacaacgatg agatgacttg aaataatata
     2101 ctcttcttat caataataga taggaagagt tttttttaga attaatgtaa caaattttaa
     2161 agtataatgg aggattaaat aatggaaaaa gtagaaagtt ttaagctaga ccatacaaaa
     2221 gtaaaagctc cttttgtaag aaagtgtagt gtacttgatg gagtaaaggg agataaagta
     2281 actaaatttg acttgagatt tttacagcca aatgtagaaa gttttggaac tgctgctatg
     2341 cacggtttag agcatctttt agctacttat ttaagagata cattagatgg tgtaatagac
     2401 ttatctccaa tgggatgtag aacaggattt tatttaatac tatggggaga tgtagatgcc
     2461 aaaacagtta agattggttt agaagaagca ttaaaaaaag ttcttgaatc agataaaatg
     2521 ccagcagcta cagctataga atgtggaaac tacagagact tatctttatt tggggcaaag
     2581 gaatatgcaa aagatgtttt agataaagga ttttctttaa atatatatgg agaataattt
     2641 aattggaaga gaagatataa gcataaatca aagtgaagtt ttaagatatt tacaatacag
     2701 aggtcaagag atagataaag accttaaata tactattgat gaatgtgtag ctataactaa
     2761 aaataaaata aatcctagat atttatctag gatttatcct attaaaataa ataaagataa
     2821 tgttgtagaa ttagagggaa caaatttaac attagagagt aaagatgtat atgaactttt
     2881 aaaagaatgt aatgagtgtg taattatggc agctactata gggatagata tagaaaaaga
     2941 aataagacga cactcttatt caaatttaac aaaaggacta ataattgatt catgtggaac
     3001 aacagcaata gaagaagtgt gtgacattgt tcaaaataat ttagaaaagg tttttttaaa
     3061 gcaagataaa tttataacta tgcgatatag cccaggatat ggagatttac caatagaaaa
     3121 aaatatagat atattaaacg ttttgaatgc tcaaaaacaa ataggtctaa caattacttc
     3181 taatggaatt atgataccga gaaagtcagt agtggctatt ataggtattt ctaatacaag
     3241 aataaataaa tctaagaaag aaaagtcgtg tgaaaattgt aaaaactata gaaactgtac
     3301 atatagaaaa ggagcgaact cttgtggaaa taagacaata tctgaaaaat aatatattaa
     3361 tatttgatgg tgcaatggga acaatgcttc aacaaaaagg gttaaagtta ggtgaaaatc
     3421 cagaagtatt tggtcttcaa aatccagata aacttataga aatacataca gcatacttag
     3481 aggcaggttc gaatgtaatt cttacaaata cttttggatg taatgaacta aaattagata
     3541 gtaagtatac tgtagaagaa gtgattgaca atgctgtttt ggtggctaga aaagctatag
     3601 aaaatgtaga taatactaaa ccaagatatg tagctttaga tattggtcct ataggagaaa
     3661 tgctagagcc tatgggtact ttaagttttg ataaagcata tgaaatattt aaaagacaag
     3721 tactacaagg cgttaaatct ggagttgatg taatagtaat agagactatg atggatttat
     3781 atgaagctaa agtagctgta ttagcagcaa aagaaaactc tgatttacct attttctgta
     3841 ctatgacatt tgatgaaggt ggaagaagtt ttactggatg tatgccagaa tgtatggtgg
     3901 ctactataga aggtctaggt gtagatgcaa taggagttaa ttgttcatta ggacctaaac
     3961 aacttttacc aatagttgaa aaaatagcat caagagctac agtccctgtt atggttcaag
     4021 caaatgcagg tcttccaaat atagttgatg gagaggctat atatgatgtt gatgcaaaag
     4081 aattttttga aggggttaaa aagttcgttg aagttggtgc tactataata ggtggatgtt
     4141 gtggtacgaa tccaagtttt ataaaagaaa tatctgagaa tataaattct gttacaaagg
     4201 gatgtatcga gaaaattgat aaatgtgtag tctgttcacc atcaaaattt gtggaagtac
     4261 aatcgcctac agttgttgga gaaagattga atcccacagg tagaaaatct ctacaagaag
     4321 cattaaaaaa tgaaaatgtt gattatgcta taaatttagg tttagaacaa gtaaatgctg
     4381 gagcacaaat acttggagta aatgttggtc ttccagagat tgatgaaaaa aaactaatgc
     4441 caaaattaat aagagagatt caagcagtag tagatacacc tttacaagtt gattcttcca
     4501 atgtagaggc cttagagcaa ggacttagat attataatgg tagaacaata gtaaactctg
     4561 taaatggcaa agaggagtct ttagaaagta tattaccaat agttaaaaag tatggttcat
     4621 gtgtggttgg acttacactt gatgaaaagg ggatacctaa aaaggctgaa gaaagatttg
     4681 aaatagcaaa aaggatagtt aatagagcag tttcttatgg gataaaacca aaagatatat
     4741 ttatagactg tttatcatta actgtttcag cacaacaaga agaagcaata gaaactataa
     4801 aagctataac aatggtaaag acactaggag taaaaactat attaggtgtt tcaaatattt
     4861 catttggtct tccaaatagg aaagcactta atgcttcttt tctaacatta gctttagggg
     4921 cagggcttga tttagcaatt ataaatccaa atgaatatag tatgatggaa gctatcaatt
     4981 cttttaaaat attaaataac acagataaag gatgtataaa ctatataaat caatatagca
     5041 atataaataa ttcaaaaaaa tcggattcat ctacaaagat tgataaagat ttgcccttgg
     5101 atattttagt tgaaagagga ttaaaagatg aagcaaaaaa tgtaacactg aatcttttaa
     5161 aagagaagga tgaaaattat atattagatg agattttaat tcctgctctt gataaagtag
     5221 gaaaaagata tgatagtgga gatatatttt taccacaatt gattcaatca gcagaaacgg
     5281 taaaagtttc attaaataca ataaaggaaa ctttattaag taaaagtagc aataatgtat
     5341 ccaaaggaaa aataatagtt gctacagtaa aaggtgatat ccatgatatt gggaaaaata
     5401 tagtaaaaat aatgctagaa aattatggat atgaagttat agatttaggt aaagatgtcc
     5461 ctattgaaga agtagtggaa gttgctaaaa agagaaatat aaaattagta ggattaagtg
     5521 cacttatgac tacgactgtt caaagtatga aagatacaat tcaggcactg agagataatg
     5581 agataaatgc aaaggtattt gtaggaggtg cagttttgac agaagaatac gctgaagaaa
     5641 tgggtgctga ttattattca aaggatgcaa agtctgctgt tgaaatagca aagttaaatt
     5701 tttagcatat aaattaaaat aaaggtttta ctaaatagga tattttatat ataatataaa
     5761 tataagtttt agatacttat gtttaaaaca ataggaatta atctataata atgagattaa
     5821 ttaatataaa aaatatttat catcaacatt aaaaagggct gggaagtctg gtataacttc
     5881 catcagccct ttttagttaa aatcatatgt taaaaagccc aattaccatt tttaaaaatc
     5941 tgtatttgct ttccatcata agtctcacca ataatatcca tatcagcaga accaatcata
     6001 aaatctacat gtgttaaact gtcatttaca ccatgttcat ctaattcttc atcttttaaa
     6061 ttatttccac cttccagaca agtcttataa gcagaaccaa ttgcaaagtg gcaagaagca
     6121 ttttcatcat atagagtatt ataaaaaatt atatccgtat tggatattgg tgagttgtat
     6181 ggaacaagag cgacttctcc taaataatga gagccttcat ccgtttcaat aagtttttct
     6241 agtgtatcga gtcccttttc agcagagaaa tctattattt taccatcttt aaatgttaag
     6301 aagaaattgt caattatgtt tccaccataa acaagaggtt ttgtactata aacagttcca
     6361 tttactttaa acttatgtgg cattccaaaa atctcttcag ttggaatatt cggattaaaa
     6421 cttattccat tagggtcttt ggatgctcca cttagccaaa catgaccctt tggcaagtct
     6481 agagtcaaat cagtttttga ggatttatat tttaaagttt taaagttatt tttatttaga
     6541 aagtccattt tagatttaag attttcatta tgttcttccc atgcttttat agggtcctga
     6601 ccatctactc ttgaacattt aaatatagca tcccacagtt tttctattgc tttatcttct
     6661 gataaatttg gaaacacttt ttttgcccaa tcttttgttg gcacagagac tatactccat
     6721 ttacatttat cagttagagt gtaagaacgc cattctttta aagcttttcc agatgccttc
     6781 tgaaaatttg caactctttc agggtcaaca tcttttaata aatctggatt ttgggcatag
     6841 actgataaaa atgccccacc ttcttttgct atatctacat attgttctgc tgtccatttg
     6901 gggaactcat gaaatgcttc atctggagca tataagtatt taataagagt actttcttca
     6961 tcagaccact ctatataaac atttttagca ccacatttat atgcttcttt aagtgcattt
     7021 cttgcaaaat cagcacattc tattggtgac tttataagta gaatttgacc tggctgtatg
     7081 ttaactccaa cttttattgc taattcagcg tatttttcta aatttctatt taaattcata
     7141 tttatcctcc tatattttta atattgaata acttataaag tattataaca ttacttatgt
     7201 tt
//
LOCUS       DAEOSP010000177         7106 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.181, whole genome shotgun sequence.
ACCESSION   DAEOSP010000177 DAEOSP010000000
VERSION     DAEOSP010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7106
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.181"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..571)
                     /locus_tag="KRH54_002949"
     CDS             complement(<1..571)
                     /locus_tag="KRH54_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG8580585.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYS"
     gene            1042..1692
                     /locus_tag="KRH54_002950"
     CDS             1042..1692
                     /locus_tag="KRH54_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBG8580586.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(1832..3208)
                     /locus_tag="KRH54_002951"
     CDS             complement(1832..3208)
                     /locus_tag="KRH54_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8580587.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(3224..5137)
                     /locus_tag="KRH54_002952"
     CDS             complement(3224..5137)
                     /locus_tag="KRH54_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBG8580588.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            5273..5905
                     /locus_tag="KRH54_002953"
     CDS             5273..5905
                     /locus_tag="KRH54_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBG8580589.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            6044..6583
                     /locus_tag="KRH54_002954"
     CDS             6044..6583
                     /locus_tag="KRH54_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBG8580590.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAKKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FEMDREGYKRYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFDKYERFYEEY"
     gene            complement(6759..6929)
                     /locus_tag="KRH54_002955"
     CDS             complement(6759..6929)
                     /locus_tag="KRH54_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG8580591.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
BASE COUNT         2624 a          970 c          940 g         2572 t
ORIGIN      
        1 ctgagtattt aactagatta gattgtattt cctttgaatc aatttgtaat aaatttatta
       61 tttctataaa tgtagaaata tctatatatc tgtgacctaa atcaaatata tctgagataa
      121 agctaggtac tttttctgga actcctggag ttatagctat ttttatattt ttatagttat
      181 tatcattcat atatggaatt ggtctaatat tagtcactcc aattttataa aataaactta
      241 tagtttctag agtagtttca tcagaatcat ttacgattag aacatttgta ccctgtggta
      301 aagatagtag attataaatt ttatcttccc taaaagttct tcttaccaca atcacttttt
      361 tcttatctag tatattattt ataattttgt ctaatctttc tactgacatt actaatatca
      421 catcatcatt tataatatca ttatcattta tttcatttat gaagtatcta ttaatattta
      481 tagaatctcc aaaaactaat ttcaaattat tttctaaaaa gtctattaaa tcttttctac
      541 tatcatttac cagagctact gattttttca tttatttctc cttatataat aaatagtttc
      601 caacaaaata gttgtataaa gcaatataat tttgataatt taaatttatc aaataaaaaa
      661 gaaagtgtct ataatgactt tataaaagtt gcattaagac actttctttc ttataattaa
      721 aaatcttgtc accaaatccc cctaattgtt gcttaaaact taaactgtat gtatattttt
      781 catttctatt cgattaaatt ataatataca cccaaaaaaa tttattttaa taaattctat
      841 tgataatata attttgtaaa tatagaatta tattaaaata aattatatat gaaaatatat
      901 atgtataagt ttattctgcg tgagtagaat gaatttgtca agtttaattt agttgtaatt
      961 ttatctttta ttaaaaataa tataaattaa aacatataaa taaaatattt tatacaatat
     1021 attgtaaagg aggtaacttt gatgaaagca gaaaaaacaa gtatatatcc aattattaaa
     1081 aataatttaa acaaaatatc aattgttgaa tataaaaagg gtcaaagatt tgtagcatca
     1141 gataaagact tacaagaagt gttctttata atagagggag ttgcattagt ggagtgtact
     1201 actagaggag gaaataaatt tcttgttgac attgtacctg aaaatgaatt tataggcaaa
     1261 attagttaca tatatgaaca taatttgaaa tgtgatatat ttgcaaagac taatataaaa
     1321 ttatttcgat ttgaaaaaaa tgtatttgaa gagttttatc tcaaaccaga ttttattgca
     1381 ctatttcata gaaaatgtac aagaagaata tatgaattat ataaaaccag aatggtaaga
     1441 gaactatttt cttgcacaga agttattgcc tattgtatat caaataaaca agaaaataat
     1501 gtctgcaata taaaagctat tcgtaatttc agtgaaatct attcaattag tagaaaaagt
     1561 agataccatt ctctgctaaa gttaactgaa aaggagatag taaaaaaaat gggtaataca
     1621 tatgaaattt taaactatga tgaattacat aaattggcat ttgaagtaaa agaatttttg
     1681 gaagatgagt aaaaattgtt acttaatcaa aataaagtat aataatttct gtaaaaaata
     1741 gaaatagtct cactgaaaat caaaaatact ttagttattt tcaatgagac tgtttagaaa
     1801 tattgtatac ttataatgta tagtataatt attaatcaat ttcctttact atattaatag
     1861 cccatttttt agataatgct ctaggaacac caaatataaa cttaaccact tcttcactat
     1921 aagtgagcaa aacgagtaca aatataggtg cacctttaaa tgcagctata aaagttagag
     1981 gaatagaagc taaccacata catcctaatt ctaaaaatag agcatattta gtatctcctc
     2041 cacttcttaa aataccaatt ataaccaatg tattaaaaga tttaagagcc attagtattc
     2101 ccattataaa gaatatctta gttatatcag aagctaagct atcagaaaca ctaaacattt
     2161 ttaataataa tggtatattt aaaattaaaa taattccaag tattaaacca gctgaaaata
     2221 caagtataga aaacttttta gcatattcaa tggctctttt aatatggtca gctccaagtt
     2281 catttccaag cataatagat gcaccagatg caatacatac agctgtcatt atgaagaaat
     2341 tacctgtact ggttgctatc tggctagctg ctatagcaga agtaccagct cttgcatatg
     2401 caacagaata caggacagtt ccaattgccc atacagagtc atttacaaat ataggaaagc
     2461 tttttgatga aaaagatttt ataaaaattt tatcaatagc ttttagattt tttagaccaa
     2521 actttaatat atagtctttt ttataaaggt aaacataagt aatcattaaa attaattcac
     2581 atattctagc aataacagta gctagagcag caccttttac acccaatgca ggaagaccga
     2641 agttaccaaa tataaatcca taatttaaaa taacatttgt tacaagtgca aatgcactac
     2701 aaatcatacc taacttagga tttctaacac ctcttgaacc agtactaaat acagtactta
     2761 cagcaattaa tggataacta aatactgcaa tactaaagta atctatacaa agttttacca
     2821 cctcagaatc atacgaaaat atgtgtataa ttggctttgg tgttagtaaa gcaggaataa
     2881 caaatacaaa acttaaaaca attgccaata cacaagtaag acctgtgact tttcttatat
     2941 tactcacatc cttctttcca taaaactgtg aaataaaaac tccagcacct cctgtgatac
     3001 cagataagga catattaaaa agaaagaaaa attggtttgc aactccaact gaagctacag
     3061 atgtttcccc caaactactt atcataattg tatctattac atttacagaa gtagaaatta
     3121 agttctgtat gataataggt atacataaag atagaagtat tttataaaat cttttatttt
     3181 cgtcttttaa agaaaatagt tcttgcaaat aatcctctcc tttttacata aattcttccc
     3241 actcatcata tagagaggca aggttatttt ctaaattaat tttttcttgg ctaacttctt
     3301 tagctttatc tggattagag tatacttctt cttgacataa aagtatatct aatttttcaa
     3361 tttcaatctc taacttttca atttcttttt ctatattttg tctttttatt ctatttttct
     3421 tttctatttc tctttgttct ctctcttttc ttttttcatc ttttatttga gttctagttt
     3481 tttcttcttt ttcttctata atactcatct cttggatttg ccttttttta tctatataat
     3541 aatcataatt tcccagatat tcagtaattc cattttcatc taaaactaaa attttatcaa
     3601 caacagtatt tagaaagtat ctatcatgtg atattgtaaa tatagtccca tcataattag
     3661 ttaaggcctc ttcaaggact tccttagaat caatatctag gtgattagta ggctcatcta
     3721 ataacaataa gtttgcattt gatagtataa gtttaagtat ggcaactcta gctctttctc
     3781 caccactcaa tgtagaaatc ttcttaaaaa cttcttcatc tacaaataaa aatgccccta
     3841 acatatttct caaagtagtt tgagtaagat gagtattatt ttcccaaatc tcatcaatta
     3901 ttgtgttatc taaattaaga gttttttgtt cctgatggaa gtaggaaaca tgtacattag
     3961 ttccaaattt aatattgcca ctaagaggtt gtagttcatt agttattatt ttaaataatg
     4021 tagatttgcc aacaccattt gcacctataa gtgcaacttt ttcccctcta tatatatcta
     4081 aatccaagtc tttaaaaagt attctttctc cataacccat agatacatct ctaatttgaa
     4141 gaacatcatt accactctgg acagaaggcg taaattgtat acgagctttt ttcctataag
     4201 cttctggttt atctaaaaca tctactttgt ctaatgcttt ttctttactt cttgcacgct
     4261 ttaggtgttt ttctctacca taagctttta gtctttcaat tgattcttct tgctttttta
     4321 tttctttttg ttggtcttca aatttcttaa gctctagttc tttttctatt ttagataact
     4381 caacaaattt agagtagtta ccattatata ctttaagctt tttattgtga acttcaaaaa
     4441 ttctatttac ggattggtct aagaaatatc tatcatgaga tattaacatg acagtccctt
     4501 tatattgttt taaaaatact tctaaccatt ctattgcttc agagtctaag tggttagtag
     4561 gttcatcaag taacaataga gttggtttct taagaagtaa ttttcctaaa agaactctag
     4621 ttttttcacc accagaaagt atgcttattg gcttatccat atcattgtca ctaaatccta
     4681 atccctttag aactcctttg gcttcagatt tatatccata tccatttaaa tctgaaaata
     4741 attctagttt atgtgaatat tcatccatga ttttttctat aattggagag tttgtttttg
     4801 tactttcttc agatatttta acctccaatt ctcttaaata ggattccatt tctatcaaat
     4861 ttttaaaaac ttctagtact tcttccaaaa tagtattatc cgaataaaaa ttggtatttt
     4921 gttctaaata tccgatttca caatctttag aagtgtaaat atctccacta tcatatccat
     4981 aaattcctga aattatttta aataaagtag tttttccagt accattaaca ccgattatac
     5041 caattttatc gccttcattt actgtaaaac taatattttc taatatagaa tctataccaa
     5101 aacttttatt taagttatta catgacaaaa caatcataaa tttctacctc cttgaactat
     5161 ctaaaattat aatgttttga tataatataa tcaaggttat atgtattagt tttgaaaaag
     5221 aaatatttta ttgtgaatca aacaaaaaag aggacacttt taggaggtga agatgttggg
     5281 aaataaaaat atatcaatgg cagttataag aaggctccca aaatatcata gatatcttgg
     5341 agacttatta gatagggata tacaaagaat atcttctaaa gaattgagtg atataatagg
     5401 gtttaccgct tctcaaataa gacaagattt aaacaacttt ggtggatttg gacaacaagg
     5461 atatggttat aatgtagaag ctcttcatac tgagataggt aaaattcttg ggttggatcg
     5521 accatacaac gcagttcttg taggagcagg taacttagga caagctatag ccaattatgc
     5581 aggatttaga aaagctggat tcgagataaa agctttattt gatgcaaatc ctagaatgat
     5641 aggtttaaag ataagagagt ttgaagtatt agattcagat actttagaag actttataaa
     5701 aaacaataat atagatattg ctgtattatg tatacctaaa aatggagcac aagaagttat
     5761 taatagagtt gtaaaagctg gaatcaaagg tgtatggaat tttgcacctt tagatttaga
     5821 agttccgaaa ggtgttatag ttgaaaatgt aaacttaaca gaaagtttat ttaccttatc
     5881 gtatttaatg aaagaaggaa agtagacaac ttaaacatat taataatgag cagttgtaaa
     5941 gagtattgag aaagtcagaa gatttaatat taaatcctct ggctttttga aatattagga
     6001 taaaaattta aataatgtat ggaaataaac aatattaagg agagtggttt ttgtggagga
     6061 attatggaaa aaggcaattg aatttcatgg tcatgaatgt ccagggttag ctataggatt
     6121 taaagcagca cttgcagcaa aaaaatattt aggatgtaaa cagtcaggag atgaggaaat
     6181 tgtatgtatt tctgaaaatg atgcttgtgg aatagatggt atacaagtag tgctaagttg
     6241 cacaatgggt aagggaaatt tactacttag gatgactgga aaatcagtat atcatttctt
     6301 taatagagaa aatggaaaat ctataaggat atatgtaaag tcaaaaccat ttgagatgga
     6361 tagagaagga tacaaaagat atttattaga aagtgatttt gaagaaatat ttgagattac
     6421 agatactaag ttaagacttc ctgaaaaagc aagaatattt aaatccctta aatgtgaagt
     6481 gtgtggtgaa tttgcatctg agcaatatat aaggattgaa gaaggaaaga aagtatgtct
     6541 tgattgtttt gataaatatg aaagatttta tgaagagtat taaaattata aattagatta
     6601 aagttaaaag tattagatat gtgctaacag tattttaaga tgaattaggt agcaacataa
     6661 taaagtatat aaataatatt aattgaatca tacataatta aaaagaggct gattagtatt
     6721 tataaaataa aatcagtctc tttatgaata acgaattatt attctggttg aggtgcatct
     6781 acaggacaag ctcctgcaca agaaccacag tctatacaag aaccagcatc tataacatat
     6841 gcgtcgtctc cagctgatat acagctaaca gggcattcag cctcacaagc accacagctt
     6901 atacaagcgt cagtaatttt atatgccatt ttgaaatcct cccttttata tgtattgtaa
     6961 ttcaataaca ttataacaaa tattattgaa tataacaatt aaattaattc tacaacaaaa
     7021 actttattat gatattcttg ttttaaagtt ttacaataaa gtttaatctg cttatatttt
     7081 tatttatttt aaagaagttt atttta
//
LOCUS       DAEOSP010000178         7046 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.182, whole genome shotgun sequence.
ACCESSION   DAEOSP010000178 DAEOSP010000000
VERSION     DAEOSP010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.182"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            40..420
                     /locus_tag="KRH54_002956"
     CDS             40..420
                     /locus_tag="KRH54_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG8580592.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            545..745
                     /locus_tag="KRH54_002957"
     CDS             545..745
                     /locus_tag="KRH54_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580593.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            941..1669
                     /locus_tag="KRH54_002958"
     CDS             941..1669
                     /locus_tag="KRH54_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8580594.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            1691..2608
                     /locus_tag="KRH54_002959"
     CDS             1691..2608
                     /locus_tag="KRH54_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBG8580595.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            2750..3934
                     /locus_tag="KRH54_002960"
     CDS             2750..3934
                     /locus_tag="KRH54_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG8580596.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKINLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            4041..4229
                     /locus_tag="KRH54_002961"
     CDS             4041..4229
                     /locus_tag="KRH54_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBG8580597.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPEDLQ
                     DLCKEAEEGCPVSAITVK"
     gene            4378..5028
                     /locus_tag="KRH54_002962"
     CDS             4378..5028
                     /locus_tag="KRH54_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBG8580598.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            5131..6069
                     /locus_tag="KRH54_002963"
     CDS             5131..6069
                     /locus_tag="KRH54_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBG8580599.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            6191..6946
                     /locus_tag="KRH54_002964"
     CDS             6191..6946
                     /locus_tag="KRH54_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG8580600.1"
                     /translation="MKKYNISENGALIKNDTIESKSESVKVVQISDTQIGSFYSTKNL
                     KKVANKINTLNPDIIVFTGDLIDYSNKNPSVDEITTILSSMNARLGKFSVFGNHDYMY
                     KLPRYYRHIMKNSNFNLLVNENKKIKLKDDKYINILGVDEILNGNPNIKYLESQIDNK
                     SFNLLLAHEPDLVDMLSKDTMNLVLSGHSHGGQIRLPIKGALVTPPYGRKYTKGFYDI
                     NGNHLYVSSGLGSTKLPFRFFNIPEIIEFNIII"
BASE COUNT         2907 a          665 c         1270 g         2204 t
ORIGIN      
        1 atattaggta tttatttaat aataaaggtg ggtattgtta tgataaataa aataggaaaa
       61 ataactctct atgtaaataa tcaagaggat gcaaagttat tttggacaga aaaattgaac
      121 tttgtagtaa cttatgaaaa ggaaatgggt ccaaatatga aatgggtgga agtagcacca
      181 agtaaaaatg aatttacaac atttgtgttg tatgataaaa atctaatgat gaagcaaaac
      241 aaagagataa atgtatctca tccatcaata attttaagta caagagatat taatggaact
      301 tatgaacaaa tggcaaataa cgaggttgag gttggggaat taatggagat gccatatgga
      361 aggatgtttt cttttaaaga ccaagatgga aatgattatt tggtcagaga ggataaataa
      421 ttttacaaag tattaaattt actcaatatc tgatataatt agagacaata attattataa
      481 actagtctag gattagaatt atataattaa aaattaagtg taaattttga gtagaggtga
      541 taaaatgttt aagaaaatgg cagtactaaa agatatagca actaaaatag gtcgtaaaaa
      601 agcgtatgaa ctattagaaa tggttgaagg taatgatgcc tttgtagctg aggtaaagat
      661 aaaaaagaat ggaatagaat ctaaaaaaga agaaattatg ttgaaagata atcaaaaaat
      721 aatattagag tatatagaag gttaaatttt aaatacatat aagttgatat actaaatttc
      781 tgagatgagt ttacataaaa ctcatctttt ttacttttca ataactttgt tttattattt
      841 aatatacatt ttatctcctt agagttgctg atatataaaa attactgata taataaaaga
      901 atggatatta atgtaatatt ttatagaaaa gaggggtatt atgcttgaaa taaaaaatct
      961 ttcctttaat gttgagtcta acaatgagga actaggaata ataaatgatg ttagcctttc
     1021 atttgagagg ggaaaactta ttgttatcac aggtccaaat ggtggaggaa aatctacaat
     1081 tgctaagctt attatgggaa ttgaaaaagc tacgagtggt cagataattt tagatggaga
     1141 agatataaca aatcttagta ttacagaaag agcaaaaaaa ggaataggat acgcatttca
     1201 acaaccacca agaataaaag gtatgacagt tgaaaattta cttacacttg ctcatggaaa
     1261 accattgtct actgatgtat gttgccaata ccttacagat gttggattgt gttcaaaaga
     1321 ctatttaaat cgtgaagttg ataactctct ttctggaggg gaaatgaaaa gaattgagat
     1381 agcaactctt tttgctagag atttaaaggt ttctatattt gatgaacctg aagcagggat
     1441 agacctttgg agctttggaa aactcaatga aagttttaag aaaatacatg aagagtctaa
     1501 tcagactata atcataattt ctcaccaaga aagaatatta gagttagctg atgaaataat
     1561 tgttctacaa gatggttcag taaaaagcca tggaacaaaa gaggctatat tacctgaaat
     1621 catgtgtcaa gttaattcaa gttgtgaatt aatgaaagac atgaattaat tagaaaggaa
     1681 ggcaaattaa atgaacaaaa tagatttaaa tcttttagaa aaagttgctg aacttcgtgg
     1741 aactccatta ggagcataca atatacgtaa aaatgggcaa ggagtagaga gacattcaac
     1801 tgaaaatgta actataattc ctaaaactga taaacctgga atagatatta tagtaaagcc
     1861 aaatacaaaa ggagaaaatg tacatatacc agtaatagta acagaggctg gtgtacagga
     1921 tatggtatat aatgattttg aaattggaga aaatgcagat gttgttataa ttgctgggtg
     1981 tggtatacat aactgtggtt gtgatgatag tgaacataat ggtatacata gatttttctt
     2041 acgtaaagga gctcgcctaa aatatgtaga aaaacattat ggtgaaggtg atggagaagg
     2101 aaagagaatt ttaaatccag ttactattgt caatatggat gaggattcat attgtgaaat
     2161 ggaaacaatt caaattaagg gtgtagattc aactaaaaga gaaatggatg caaacctagg
     2221 taaaggagct aaaatagttg tatttgagaa attattaact catggtaatc aagttgcaga
     2281 atctaatatg aaggttatct tagagggtga aaactcttct gctcaagtag tttctcgtac
     2341 agttgggcaa gataattcta agcaaaagtt taatccttgt gttattgggg attccctttg
     2401 ttctgcacat gttcaatgtg attcaataat tatgggaaat tctcaaatta gtgctatacc
     2461 tgaaattgct gcaaatcatc cagatgctct tttagtacat gaagcagcta ttggaaagat
     2521 tgcaggagac caaatactaa agatgatgac attaggtctt actgaagaag aagccgagca
     2581 acaaatactt aattgcttct taaagtaaat taaagatttt atttaattta atataattaa
     2641 tatagtataa tatagtgaat aagtctaaaa tatttttatt tttaggctta ttttctttat
     2701 gtaagaaaag ctattagaga aattatattg taattaggat gtggaataaa tgaaaaattt
     2761 acttataaga aatgggacaa taatatctcc aacagatggt atcaaatttg aaaaaagaga
     2821 tatcttggtt ttacatggga aaatatatgc tattggattg aaccttaaag agaaaataag
     2881 aaaagaaaaa ttacttacaa ataaagagtt cgagataata aatgccgata atatgtatat
     2941 ttccccagga tttatagatg ttcacaccca ttgttataag agaaaattac ctacaggtat
     3001 ggattctgat tcaattggaa ttgaaaaagg ttctacagtt atatttgatg caggtacagc
     3061 tggtccttca aactactatg attttaaaaa aaagtatatg gatgagtgta aaacagaagt
     3121 atattctttt ttaaatgtta caaatgaggg actaaatata ttgaatgaat caacttcttt
     3181 agatgttata aattttgaca aggttgagtc aattgtagaa aaaaatatag aaaagataaa
     3241 gggtataaaa gtaaaagcga gtaaatttca atctaatgaa agtggtatag atataattaa
     3301 gaaatcaaag caagttgcta aaaaaataaa tttaccctta gtggtatata taggagagta
     3361 tccatcatat atagatgaag taatgaatat tttgggaaaa ggagatgtgg ttacaccagt
     3421 atatggaaat agtacaaatg gaatccttaa agaatctggc acattaagaa aatcagtaat
     3481 aaatgcaaag aatagaggag tattgtttga tgtatgtcat ggagtggacc aatttgattt
     3541 taaagttttt aaaaaagcaa taaagcaagg tttagagcct gacctaataa gtactgatat
     3601 gcatataaaa aacatgaaag acgtaaacta ttctctttta aatgttataa ataaaattat
     3661 ggaacttggg atatccctag aaaagtgtat agaaaaagta actgaatacc caacaaagat
     3721 tttcgattta catggaaata aaggcagaat aaagattggt ggagaaggtg attttacaat
     3781 atttacaatg gaggagtgta atgatattat aaaagactac aataataatg aacttgtttt
     3841 aaataaaaaa ttaagattgc aatatactgt aaaatcatgg atgaaaagca gtgaagttta
     3901 tagacatgga tacgaaaata ctataataaa ctaaacaata gtgataaaat aataaatata
     3961 tgtttaaatt ggtatatatt ggataaatat ctaaggaaac aacaattaaa aaataataat
     4021 tttaggggag gtattttagt atgaaagcat ttgttgataa ggatgtatgt gtagcttgtg
     4081 gagcatgtat aggaacatgt ccagacgttt ttagtatgag cgatgaaggg cattcagtag
     4141 caattgaagg agaaatacca gaagatttac aagatttatg taaagaagcc gaagaaggat
     4201 gtccagtttc agctataaca gttaaataga gctgtttaaa tttattttta aaataaatat
     4261 ataagattta attggtactt aagctatagt attagaaatt tctattacta tagcttttta
     4321 aaatagataa acttaatata taagatatta ctttttacaa atatggagga ctgaaatatg
     4381 aaaaaaaatt atgaaatagt attattcgat ttagatggaa cacttacaga ccctaaagaa
     4441 ggaataacaa agtctattca gtattcttta aatagctttg gcataaaaga agatttggaa
     4501 aacttagacc aatttatagg accaccatta catgatactt tcaaggagta ttataaattt
     4561 gaagataaaa aagctaaaga agctgttgaa aagtatagag aatattttgc tgataaaggg
     4621 atatttgaaa ataaaattta tgagaatatg aaagagatat tagaaatgct ctataaaaat
     4681 ggtaaaatat tattagtagc aacttctaag ccaacagttt ttgcagaaac tatacttaga
     4741 tattttgata tagatagata ttttaaatat atagcaggta gcaatcttga tggtacaaga
     4801 gtgaataaaa atgaagttat acaatatgta ctagacctat gtaatgtaaa agataaagat
     4861 aaagttatta tggttggaga tagaaagtat gacataattg gagctaaaaa aataggtata
     4921 gattctattg gagtattgta tgggtatggt tcttttgaag agattagtga aagtgaacct
     4981 acttatattg tcgaaaatgt agagtctata aaagatattt tgttataaat atcttttata
     5041 gacaaaaata ttacaaaatg gtgtcgcata tttaaaaata tgatattata gttaaggaag
     5101 tcatttaaaa aagaggagga tgcacttaaa atgaaatcta ttttaattat gggtggaagc
     5161 gacttcatag gttctgcact tgctaagcgc ttgattaaat gtgggtatca aattgatata
     5221 cttacaaatg gtaaaaagga aatagattat aatggtttta aagaacatct aatttgtgat
     5281 agaaaagttc gaaaagatat ggaaaacatt atcacaggaa gaaaatatga ttacatatat
     5341 gatatgacag catatacaaa agaagatgta agtaatctga ttgattttat aagcatggat
     5401 aacttaaaaa agtatatagt attgtcagca ggagcagtat ataaagatag tggaagaaat
     5461 ataaaagaag aaaatgagaa aggtgaaaat gaaaactggg gaaaatatgg gttaaataaa
     5521 aaagaggctg aagattttat tataaatagc cctataccat atataattat tagaccaact
     5581 tacatatatg gtgaaaataa taatttatat agagagtatt atttctttga aaaaatagaa
     5641 aaaaatgaaa agataccagt accaaaagga aaacaagtta gtaatcaatt tatatatatt
     5701 ggggatttgg taaaggtatt ggaaagcata atgaaaaatc ctcatgtaag agaggcctat
     5761 aatgtgacaa atccacagtt aatttcttgg gatgacctta tatatacatg tggagaaatt
     5821 attggcaaag aacctataat aaaatatgta gatatggaaa aagtagagtt tagagaaaga
     5881 acgtatttcc catttagaaa tatagacttt aatcttgata taaataagct tattgaacat
     5941 gggctttata taccaaacgt gttactaaaa gaaggtctta cagcaacata taaatggttt
     6001 tcagcaaata agccaaaaat gcatgacaga aaaatgaaca aagttgagtc tgtaatgcaa
     6061 atagtttaaa aaaataaata agtaggtaga tattttgaac tcaaaaatta agtatttttt
     6121 aaaatttttt atcttaatat ttattttgat ttttatatac tcagttgcga tagagccgag
     6181 tctattaatt gtgaaaaaat ataatataag tgaaaatgga gcattgataa aaaatgatac
     6241 aattgagagt aaatctgaaa gtgtaaaagt agttcaaatt tcagatactc aaataggtag
     6301 tttttattca actaagaatc tcaaaaaagt agctaataag attaacactt tgaatccaga
     6361 tataatagta tttactggtg atttaattga ttactccaat aaaaatccaa gtgtagatga
     6421 aattacaact attttatcaa gtatgaatgc taggttggga aaattttcag tatttggcaa
     6481 tcatgattat atgtataaac ttcctaggta ttataggcac attatgaaaa attccaactt
     6541 caatctttta gtaaatgaaa ataaaaaaat aaaattaaaa gatgataaat atattaatat
     6601 attaggtgtt gatgaaatat taaatggaaa tcctaatata aaatatctgg agagtcaaat
     6661 agataataaa agttttaatt tattgttagc acatgaacca gatttagtag atatgcttag
     6721 taaagataca atgaatttag ttctatctgg gcatagtcat ggtggtcaaa taagattacc
     6781 tattaaggga gcattagtta ctcctccata tggaaggaaa tatactaaag ggttttatga
     6841 tataaatgga aatcatttat atgtgagttc gggtcttgga agtacaaagt taccatttag
     6901 attttttaat ataccagaga taatagaatt taatattata atctaacata aaatatttag
     6961 aaaatataat tttgcttttt ataatatgta gatgtatata ctgtaaaaat ggattaaacc
     7021 agtagattga atgaattaat agatta
//
LOCUS       DAEOSP010000179         7046 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.183, whole genome shotgun sequence.
ACCESSION   DAEOSP010000179 DAEOSP010000000
VERSION     DAEOSP010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.183"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(201..1040)
                     /locus_tag="KRH54_002965"
     CDS             complement(201..1040)
                     /locus_tag="KRH54_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBG8580601.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(1064..1822)
                     /locus_tag="KRH54_002966"
     CDS             complement(1064..1822)
                     /locus_tag="KRH54_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8580602.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1869..2342)
                     /locus_tag="KRH54_002967"
     CDS             complement(1869..2342)
                     /locus_tag="KRH54_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8580603.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSYNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDIKCFIQIVPDAKKIDVGTLL"
     gene            complement(2371..2787)
                     /locus_tag="KRH54_002968"
     CDS             complement(2371..2787)
                     /locus_tag="KRH54_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8580604.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2834..3886)
                     /locus_tag="KRH54_002969"
     CDS             complement(2834..3886)
                     /locus_tag="KRH54_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBG8580605.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECNECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKVIAVDVFDEKLDLAKGLGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4282..4962
                     /locus_tag="KRH54_002970"
     CDS             4282..4962
                     /locus_tag="KRH54_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBG8580606.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5095..5415)
                     /gene="sugE"
                     /locus_tag="KRH54_002971"
     CDS             complement(5095..5415)
                     /gene="sugE"
                     /locus_tag="KRH54_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBG8580607.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5750..6577
                     /locus_tag="KRH54_002972"
     CDS             5750..6577
                     /locus_tag="KRH54_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG8580608.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGDLIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
BASE COUNT         2383 a         1282 c          837 g         2544 t
ORIGIN      
        1 gaaaaagcac ctcattctga ttaaaaatct ttctgagata ctttttaaaa tattaaatac
       61 tacatatagc ttacataagt ttaaaattta atctattata aaattaccct aattgccaaa
      121 ctttttaata taaattttcc aacatcctac aaatataatc aatagatata gaatcctaac
      181 ttacaccaat atatatttat ttaaaatagt cctaaaatac ctgtaatagc tcccactact
      241 atcagtataa acattatttt tattggagaa agaccttttt ttaaagcaaa gagtactaat
      301 aaagtaagta ccaaaggaat taatcctgga aatatcttat caagtaaatc tgtttgaaga
      361 ctaaatttaa aatcaccact agtataactt attggtgtag ataaattgat aaacctacct
      421 acaagccccc ccatgactaa ggtacctagt atagaagccc cttctataac cttatttact
      481 attccacctt gaagtatact ttcaacagct tttttcccat acttatatcc atttgtccac
      541 attccaaaag aaattgaagt cattattata tattgagcta ttacaaataa tataggtcct
      601 gcaattgatg ctcctccctc agttaaacca atacaaacag ccaacactat aggagttata
      661 actccttgag ttaaagtatc gccaattccc gctagtggac ccatcaagcc tgttttgata
      721 gcattcatac catctccact tatttcatct gctccattag ctttttcttc ttccatagct
      781 attgttattc catgtataac acatccacat ataggttctg tgttaaaaaa tgctagatga
      841 gtctccactt cttgttttaa ctcttcctta tctggatata attttttaag tactggcaac
      901 attgaatgtg aaaaagctgt tgattgcaat ctttcataat tgtaattaga ttgtgcaaag
      961 aaaaaccatc ttaaccatga ttttattaca tctttcttgc ttaacgtttt cttagatact
     1021 tcatttttta tatctgacat aatattttcc ccctcacatg tatttaagat gtagcactat
     1081 cttttccgta cttaattgaa gtaattgtaa gagcaagtat tactccaata acagatatta
     1141 ctattatatc tactttaaaa taaacagcca ttagtattcc taatatgaaa aagggcatag
     1201 taaacttgtt tccaatcaat tttaaattca tagcaatccc aagtgctggc aacatagccc
     1261 caattacatt aaatacatgt aacacattat cattcattac ttccaacata ccatctatcg
     1321 catttggacc aaaataaaca actaataatg ctggtataaa actcattgca aataacagta
     1381 tctgtggtgg tactatattt ataaatgcta ctctatcaac attccctttt tgaacctctc
     1441 tatcagccca atgtgcaaaa aaagaattta ctgtcatctt tccaatccaa agtacagttg
     1501 caacagttcc caatggtaca gctattgcca ttgcagtact tacatctagc ttagatgcta
     1561 tagctaaagc agtacctaca tatcctgcaa aagctgggtc ccccatctga gctccccctg
     1621 ctgatataaa acctagatat atcatattta tacctgctcc tataattgct ccttgaacag
     1681 ggtctcctaa tataattccc gttacaaatc cagccaatag aggtctattc catgtaaaaa
     1741 atccaacata agtcaaccac ggactccaag aaagataata aaaaattgct attaatactg
     1801 cttgaattag tgaaatgtgc atattcttcc tccctatttt cttttaaaga tttacaagat
     1861 agtcttcttt ataataatgt tcctacatca attttctttg catctggtac tatttgtata
     1921 aagcatttaa tatcttttga tattagctct ctaaatattt ctttttcttc atctgacaca
     1981 gatatatttt tataaaaact ttttctacca agccctgctc ccatacctcc aacattcaat
     2041 tcctttaaat ctaccccgct atttactaaa cctaagacag ttttagggta tttgaccaat
     2101 actattaaat tttcatctcc attatatgat tttaagactt cagcacctga ttctacatca
     2161 tgtacttcaa ccttaacagt tgatggagct gccatttgta aaacttgaac tataaatggg
     2221 tcctttacta ctaaatcatc cacgattact atcctttttg ctgatgtaat cttacaccaa
     2281 gcagtaataa cttgcccatg tattaatcta tcatcaattc ttgttaatgc tatattactc
     2341 ataattattc ctccagattt ttaatttatt ttacaacttt aaaacagttt taacatcttt
     2401 gattcctgat atcccaactt cctcacataa attcttcaag tcttttaact tcatagtatc
     2461 tctgtttaaa aataactcta aaagcatagg taaatttaca cctgtcaaac attctaactc
     2521 acatacctta gcaaatttat ttattataat agcagatgta ttaaatggag ttccgccata
     2581 aaaatcaagc aatactaaaa ccccttcatc cttatatttc tcaattccat cttctaactt
     2641 tctactaaac tcatctatgc tgtcaccatg gtttaatcct aaagtttcta cattttcctg
     2701 tttgcctact attaattcag cactttctaa cattgcttta gccatatttc catgtgtggc
     2761 tataattatt cctgccatat gaatcatacc tttcttaacc ttttttatat ggattattat
     2821 ttagtactct aatctaatta tctatattaa tcattatctt accaaagaaa atatcccttt
     2881 tgtatacttt ttcaaaataa gctggtactt cttctaaatt tattctatgt gttactaatt
     2941 tttcaacatt catatcaccc ttttgcatat gatacaatcc agcactccat tcttttcctg
     3001 ggaatggaaa ggagtttcca aaccaagttc cctttacagt taattcactt cttactatct
     3061 tttcaaattg ctctctagtc aaagctacat ccccatatgg tacacctgca tacaatactg
     3121 tacctccctt tttagctagc aacagtactt gaccacatgt cagtggagta ccagccgatt
     3181 caataacaat atctgcacca tctccatcag ttaacctttt tatttcttct acaatattct
     3241 tttcttttgc atttatacaa atatctgccc ctaacccttt tgccaagtct aacttctcat
     3301 caaatacatc cacagctatt acttttgtag aaccaaatat ttttgcccat tgtattgaaa
     3361 ataatcctat tggacctgta ccaagtacta caacagtatc tccaacttta gcttctgacc
     3421 taaaaagacc atgtcctgct atacatactg gttctagcgc agcggcagtc tcataactta
     3481 tctcatctgg tatttttatt aaatttctct ctttcacctt tgtatactct gcaaaacaac
     3541 ctccaagttc tttatttcct attattgcta cattattaca tcttgaatac aatccttttt
     3601 tacattcatt acattcaaaa catggcatgg ctggacaaac agcaactcta tctcctattt
     3661 taaaactcct tacttcttta ccaacctgtg ccacttctcc agaaaattca tgccctaaaa
     3721 tttctccaac catatgtgga cctgtctttg aatattttga tatatcagaa ccacatattc
     3781 cagctacttt tactttaatt attacatcat ctttttctaa tatttttggt acatccacat
     3841 cctccactct tgtgtctctt ataccataga atctaactga cttcactatt aaccacccct
     3901 actttttatt tgagaatata tccctattgt ttatatgtta aatatctaaa gcaactttca
     3961 tgccaatata tgtaagcatt gtattcatgc attttataaa attatcatta aatgtgtgtc
     4021 aaagtgtgtc aaaagtttct tatattaaaa cttatgtgtg tcaatataaa cgacactaac
     4081 attttatatt agttctctta ttataagttt tataaaatgt actttttttg ttatttttat
     4141 aaaattgttc ggaaattccc cttctatttg tgatataata ttaaatataa ggtatcaata
     4201 taaattttca tgcgaatatc agaacttttt tgacactaat gccttattca atataataat
     4261 ttaacttttt ggaggttgtt tatgaaaaaa gtgtatcaag gaaagactaa agatgttttt
     4321 gaattagata atggaaatta cttacttaaa tttaaagacg atgtaactgg taaagatgga
     4381 gtatttgacc caggcgaaaa tagtgtaggt ctaagtatag atggaattgg tagagctaat
     4441 ttagagacat ctgttaagtt ctttgaaatt ctaaacaatg ctggaattaa aactcattat
     4501 gtaagtgcta atttagaaga agcaactatg gaagttctac cagcaaaagt ttttggtaat
     4561 ggattagaag ttatttgtcg ttatagagct gttggtagtt tcttacgtcg ttatggttca
     4621 tgtgttgaag aaggtgctaa actagattgt tacgtagaag caactttaaa agatgatgac
     4681 cgttgtgacc ctcttataac atctgaaggt ttagaagctt taaacattat gactcaagct
     4741 caatttgatt caatgaaaag catgactcaa aaaatttcta acatagttcg tgatactata
     4801 gctgaaaaag gtcttgagct ttatgatatt aaatttgaat ttggattcta caatgatgaa
     4861 gtaatcttaa tagatgaaat agcatctgga aatatgcgtg tttataaaga tggtgtaata
     4921 gttgacccta tggatttaac tgctctttta ttagataaat agtatataca tataatttac
     4981 aaatataata aacttataaa taagataagc tatgaagtaa agacttttta aatgttgatt
     5041 atttttaaac attttctcgc ctttaatttc atagcttatt taaattaata tactctaatt
     5101 agtagataat agctttaacc ctacaattcc caccacaata aaacctatac atgcaattct
     5161 tgatatacta ataggctcat ttaaaatagc catgcctaca atagctgtac cgacggtacc
     5221 aattccagtc catattgcat atgaagttcc aagtggaaga tgttttagtg ctaatgacaa
     5281 aaaataaaaa cttacaatca ttccaacaac agtaagtata cttggagtta atttagaaaa
     5341 tccatgtgaa ttttttagtt gaaaagccca aaacacttca aacaaacctg caacaaacaa
     5401 aaataaccat ttcataaatt catccctctt ttcaaatatg tctcttattt ttccaataaa
     5461 gtgttcacaa caaaaactat gttgtgggtt ttatctcgga ccagaccaat cattaataat
     5521 tgcggaaccc tataaaactc tattaattat tctattttat ctataataat actatcaaca
     5581 tatataaaaa aagtcaatat atgggttcca tatataataa aaataacttt tatatacctt
     5641 ttaatattct tattcacttc tctccatacc catttgggtc aatctaagaa tatctaattt
     5701 ttatttaata tgttataata acataaaaat attgaaagga gtaatttcta tgaatttcta
     5761 taaaattgca gtatgtcaga tgattactac agaaaataaa attgaaaata tcaatcatgc
     5821 tgtagatatg gtaacagaag ctgccatcaa tggagctaaa attgtagttt tgccagaaat
     5881 gtttaactgt ccgtatgaaa ataaatattt tcccaaattt gctgaagaat atccaggtga
     5941 aacaacaacg atattaagta aattagctga gaaacatgga atttatctag tttctggtag
     6001 tattcctgag ttagaagatg gaaaaatcta taatacttgc tatgtatttg ataaaaatgg
     6061 agatctaatt ggaaaacatc gtaaaatgca cttatttgac attgaagtta caggaaaagt
     6121 tagttttaaa gaatcagata ctcttacagc tggaaatgat gtcactgtaa tagataccga
     6181 atatggaaaa gttggtatag ctatttgcta tgatattcgt ttccctgagt tatcacgttt
     6241 aatggctctt aaaggtgctg aaatcgttat cttacctgct gcatttaata tgactactgg
     6301 tcccgcccat tgggagttat ctataagaat gcgtgcttta gataatcaga tattctatgt
     6361 tggtgctgca cctgctagaa atatgaatgc ttcttatata gcttttggaa attcaagaat
     6421 ttcagaccct tggggaagaa taattgctca agctgatgaa aaagaatgta ttatttatgc
     6481 tgacattgat agagatttaa ttcctgacat aagacaacag ttaccacttc ttaagcacag
     6541 aagaactgat ttatatgaac ttaatacttt gaaataaaac agaacataac cccataaatc
     6601 ataaattaag tgttaaaaaa atttatgggg ttctcattaa tttgatttaa aattaatttc
     6661 ataaaaataa tgctaaaatt aatttaatta aataatacta ataattacta atgattatgc
     6721 taattatcag catttactaa tttaatcact attagttatt tcttttatta aatatcttta
     6781 cttttttact tattatgtct tctagcttta catttggatt atggtatttc tttctatttt
     6841 tagtcacatc tttctattga attacttgct taaaacacca atgcacacat gccagataat
     6901 gttcacaatt atgtagaaat cttgctctac cttcttcaat gttgatatta tctctcctag
     6961 ttgatattat ctttcctaaa atttctttta attaaaaaat aacattagtt gatatatcaa
     7021 gtatatttta atcaactgaa aaaaac
//
LOCUS       DAEOSP010000180         7026 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.184, whole genome shotgun sequence.
ACCESSION   DAEOSP010000180 DAEOSP010000000
VERSION     DAEOSP010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.184"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            335..844
                     /locus_tag="KRH54_002973"
     CDS             335..844
                     /locus_tag="KRH54_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBG8580609.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            872..1066
                     /gene="rpmI"
                     /locus_tag="KRH54_002974"
     CDS             872..1066
                     /gene="rpmI"
                     /locus_tag="KRH54_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBG8580610.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1122..1478
                     /gene="rplT"
                     /locus_tag="KRH54_002975"
     CDS             1122..1478
                     /gene="rplT"
                     /locus_tag="KRH54_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBG8580611.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            1788..2333
                     /locus_tag="KRH54_002976"
     CDS             1788..2333
                     /locus_tag="KRH54_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580612.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKKDEGK"
     gene            2768..4693
                     /locus_tag="KRH54_002977"
     CDS             2768..4693
                     /locus_tag="KRH54_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG8580613.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWTINDKFSKTIEVE"
     gene            5094..6998
                     /locus_tag="KRH54_002978"
     CDS             5094..6998
                     /locus_tag="KRH54_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBG8580614.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVNVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSNSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         2963 a          712 c         1328 g         2023 t
ORIGIN      
        1 tcatgtagaa tatacatata taaaaggatt ttgggtataa atttaaaaat taatagcaaa
       61 atgagtagta tagacatata attagtagta ggaagtaatt aaatttttcc taaacaaaaa
      121 aaatagttgc aaataatttg attaagtgat atagtaaata aggtaagaat aaaacaaaga
      181 agaagtccgc ttctcacctt acgccggagg ttagtaaggt aaatataagt taaagcttaa
      241 ttttaattta agtttgttta aatttggtaa cttatattgc ggatgattac gtcatccgct
      301 ttttatttta taaaaatttt ggaggtgtcc tacaattagc aaagaattag caatcaatga
      361 acaaataaaa gataaggaaa taagagtcct ttcaccaaca ggtgaacaac ttggagtaat
      421 gcctactaaa gaagctcaag caatggctaa tagcaaaaac ttagatttgg ttcaaatctc
      481 gccgaatgca aatccaccag tatgtaagat aatggattat ggaaagttta gatatgagca
      541 agcgagaaaa gagaaagaag ctaagaaaaa acaaaaaact atagttgtaa aagaagtaag
      601 acttagacca ggtattgaac aaaatgattt aaatacgaaa gctaataatg ctataaagtt
      661 ccttaaaaaa ggggataaag tcaaagttga acttagattt agaggaagag aattaggaca
      721 taaagacata ggtaaagaag ttatgcttaa gtttttagat ataataaaag agtttggaga
      781 accaacaaaa gcccctgcat ttgagggtaa taatatggtt gtaattatag atccaaaaaa
      841 atagatgatt aactgagagg aggaatttgt catgcctaaa atgaaaactc atagaggtgc
      901 agcaaagaga ttaaagaaaa caggaactgg aaagttaaag agagctaaag cttttaaaaa
      961 acatatatta actaaaaaat ctgcaaaaac taaaatgaac ttaagaaaat caactttagt
     1021 aagtgatgga gatgctaaga gaatagcaca attattacca tactaagata cagattgtaa
     1081 ataataacta tagaagaggg atataaaagg aggtcataac gatggcaaga gttaaaaaag
     1141 ctatgaacgc tcgtaaaaag cataagaaaa tattaaaact tgcaaaagga ttcagaggat
     1201 ctagaagcaa attatataga ccagcaaata cattcgtaat gaaagcatta aaaaatgctt
     1261 atataggaag aaagttaaag aagagagact tcagaaaact ttggatacaa agaataaatg
     1321 cagctgctag aatgaatgga atatcttatt caagattaat gaatggatta aaattatctg
     1381 gtgttgaagt taacagaaaa atgttatctg aaatggctat acaagaccca gaaggatttg
     1441 caaaattagc agaagttgct aaagcaaaat tagcttagta ttgaatcatg agatgtaaag
     1501 tctttgagtt atataaataa ttcaaggact tttttatttt ttgaaaaata cttattagtg
     1561 tcatgtacaa ccaattttat aatgataaat aaaataagca atacaataga attttatgag
     1621 atttattata agaattgtta caattattta tgatagctat aaaaagtcat agtaaaaatg
     1681 atattgatgt atgttgatat atgtataaag tttccaatca atcttgaatt aatattatca
     1741 acatgataat attatcttat taataatata aataaggggg acataatatg aatgaaaaac
     1801 taatggattg ttttgtaaac ccaattaaat gcaagttatt gattgaaatt ttttcaaata
     1861 atcgaactac agctaaacaa ttaggagaaa aatttcaaga tatatcaaag gcaacacttt
     1921 atcgttacct taataaaatg ttgaaagatg atattataaa agtggttgaa gaaaataaaa
     1981 taaggggaac tgttgaaaaa gtatatgcta tgaagttaga tatagctgat gatgtgaaca
     2041 aaattataga tgagaactct ggagaagaat atgctcaact gtttactcaa tatatgatgg
     2101 gatttttaag agaattttat gaatacgctg aaagaaagga aaaggatatc ctaaaagatg
     2161 gttcaggatt ttctgtctgt ccagtatatg ctagtattga tgagatagtt gaagtgagta
     2221 aaaaaattgc tagtatacta gaacctctaa gaaataactc acatgaagaa ggtagaagaa
     2281 tgcattctat aggagtgata attactccac ctaaaaagga tgaagggaaa taaatctgat
     2341 ttacatgggg tagagccaat ttatacaggg aatcgtttta tgatttacat atgtatccag
     2401 agcaaagtat ctctgcttgg attgtacttc attgtaccat ttcatccaat gacttggagt
     2461 ggtacaatga agttttttaa tttttcatat taatgctaaa gaaatgaact ttagaccaca
     2521 tatgcaatta ggttgcatta tacaattaga ccacatatac aaaataaaaa tcagtatttt
     2581 tatagcagat aaattgactg tcattcttct taaaaatcat ctattagtta ttgttagcat
     2641 attgaaagaa attatagtaa tctattgaat aaatgaatat tgcctataat catataaact
     2701 gctatagttt aaaataaagc aggacgttaa attttatagt aatctaaaaa tttaagaggg
     2761 ggattttatg aaaataaaat ttgttattat gccattggtg atatttttaa tggtattatt
     2821 tgtggggtgt caaagtacaa aagatacaga agttaaagca aaagatacag aaattaaaat
     2881 attagggaca ggtgatttac attcaatttt gacagactct atggtttcgt atgtaaatga
     2941 ggaaagggaa aagaataaaa atctgcttat ggtagatgcg ggagatttct atggtacaca
     3001 aagtagagaa atgtgggaat ggtcttctgg aaaaaagtta attaatatta aaagaaatgg
     3061 tagagcagag tatgatgaca taattaagag tagtaaagat gaagtaccta tagttaaaga
     3121 tatggcaaag ttgaaatatg atgctgtagt tttgggagat aatgaatttg tttcaaatga
     3181 taagcaaagt cttgataaac tggtttctga ttttaaaaat aataatatgc cattggtatc
     3241 agcaaatata tatgaacaat ctggagaaaa ttatgtacaa ccatatgtaa tgaaaaatat
     3301 taagacagat gagggcaatg taaaagttgg aatactaggt cttactataa aagaagtagg
     3361 ggaaaccctt ggtcttgagg attttgaaaa ggataaaaaa gctagagagc ttggagaaca
     3421 agctgattac aaaggaaaat tatatgcaaa tgatttagta gaagatgcaa aaaaatggat
     3481 aaaagttatg gaaaaagaaa gtccagacat aatagttgca gttgtacact caggggaaga
     3541 acctaaaaca gatagaaatc caggaaatag aataaaagag ttagcaacta cagtggatgg
     3601 aatagatgca attgtagctg gtcatactca tgaaaaaata gatgaacaca aatataaaaa
     3661 taaatctgga gatgaagtaa ttgttacaca accaggttgg catggagata gagtatcaga
     3721 gattaatttc aaactaaata aagagaatgg aaagtgggac ataaaggaaa agtctagtaa
     3781 attaaaaata attgataaag aagtaagtat agttgctact gcagatttac attcacattt
     3841 ctctgataaa ttaacagttg accttgttaa tgaacgaagt aacccaattg aacctgttgt
     3901 agtagatgca ggcgacttct tagaccctca aacagatgaa atgactcaat ggtataaaga
     3961 gtggaaaagt ataagagata ataatagtga taaaacgatt agtagatgcc caatggtcat
     4021 ggatatgaat cagggaatgt atgatgcagt agtacttgga aatcatgaat ttgtatctga
     4081 aaaagatgaa ttttggtcag atgaacgcct tttagatgct gttatagagg attttgaaca
     4141 ggtggcaatt tctgtgttat cggcaaatat atataggcaa tctggtaaaa attatgttaa
     4201 accatatatt ataaaagatg tggagacgag tgaaggtaat gtaaaagtag gaatattggg
     4261 acttactata aaagaagtag gtaaaggtct taaaaatgta aacttacaag agcaatttca
     4321 atataaggat aaattgtatg caaatgattt agtggaagat gcaaagaaat gggtaaaagt
     4381 tatgaaaaag aaaaaaccag atataatagt tgcagttgta cattcaggag aagaaccaaa
     4441 aaatccaaaa catccaggaa atagaataaa agaactagca actacagtag atggtattga
     4501 tgcaattgtt gctagtcata ctcatgaaaa aatagatgag catgaatata aaaataagtc
     4561 tggagaaaaa gttatagtga cacaaccagg tgagcatggt aaatattatt ctaaaattac
     4621 ctttaaagta aataaagaaa agggttcgtg gactattaat gataaattca gtaaaacaat
     4681 tgaggttgaa taactttata aaatttatag ataaagtaaa ttatctgata ttggcatgat
     4741 atataagata aagtttctaa tattagtata atgcataatg tgaaatattt taataataag
     4801 agaataaaca agacaaataa aaatatttta acatgtaaga tatagtatga actattatac
     4861 taatgcttac tttataattt gaacatgtta gaataagtct tatttgaaaa ttttagtgta
     4921 ctagagtaag tattatttta ctagtaaaaa atattgagaa ctatatgtag atttgcttaa
     4981 aaaggaattt acatatagtt cttttcgtat aataaatagt acaatcttat atggtaaaga
     5041 taaagtgtga tattaaattt tattatcatc caaaatctaa ggaggagtct cttatgaaaa
     5101 taaaatttat tatagtagca tcaataatat ttttaatagt attacttgtg gggtgtcaaa
     5161 gtactaaaga cacagaaatc aatgtactcg ccacaagtga tttacattca attattccag
     5221 ataatttaat ttcctatgta gaagaggaga gaaaatatga tgagaatcta cttatggtag
     5281 atgcaggaga tttttttgac atgcaaagtg gtgagatgaa taagtggttt actggtcaaa
     5341 aattagtaag atttaaagat gggataccag agtacaaaaa aatagctgaa ccacgtgaag
     5401 gagaagttcc attggctaaa aaaatggcaa agctgaagta tgatgcagtt acatttggga
     5461 atcatgaatt tgtatctaat gacaagcaga gccttgataa attggtttct gattttaaaa
     5521 ataacaatat accattagta tcagcaaacg tatatgaaca atctggagaa aactacgtaa
     5581 agccatatgt aatgaaaaat ataaatactg acaagggaac tgtaaaggtt ggtatacttg
     5641 gtctgactat aaaagaagta ggggaacgtt caaggtttaa aagttttgaa caagataaaa
     5701 aggctagaga acttggggaa caacctcaat acaaagggaa attatatgca aatgatttag
     5761 tagaagatgc aaataagtgg gtaaatgtta tggaaaaaaa agaaaagcca gatataatag
     5821 ttgccattgt acattcaggt gaagagccaa aaaatcctaa aaatccagga aatagaataa
     5881 aagaactagc aacaactgta gagggaatag atgctattgt agcaggtcat acacatgaaa
     5941 aaatagaaca gcatacatat aaaaataatt ctggtgaaga agtaatagta acacagcctg
     6001 gagctcatgg taatagtata tcaaagatta attttaagtt ggataagaag aatgataagt
     6061 ggtttataaa agacaaatat agtgaattaa aggtttttgg aaaggaagta aatataatta
     6121 caacttctgg tctgaatcca aaattttcag atgaaatggc agttaatttg tttaatgaaa
     6181 gagatagaga tgttcaacct atctttatag atactggaga ttttttaaat actaatacaa
     6241 aagaaatgac taagtgggct aaagagtggc aatacaatga aaataaaggc atctataaaa
     6301 aaataaatga atggccaatt gtatatcttg gttatgatgc tgtagtactt ggtagccatg
     6361 aatttacaga ggataaagag acttttgagg aaaaaaaatc tacattggat tatattatag
     6421 aaaactttga agaaatgaag ataccagtat tatcagctaa tatatatgat gaatctggag
     6481 aaaattatgt aaaaccttat ataattcata atgtagaaac tagggaagga aatataaagg
     6541 ttggagtatt aggtcttact actactgata aggatgaaga agacttaagt aactcaagct
     6601 taagcaaaga ctcaaagact tcgaaaattc ctgaagaaac aggaaagtta tatactaata
     6661 atctagtaaa agatgcaagc gaatgggtaa aagttatgca aaaggaaagt cctgatgtga
     6721 ttgttgcagt tgtacattca gataataata gtactctaaa aaatccaagt agtgaagtca
     6781 aaaaacttgc aatgtcagta gatggaatag atgctattgt tgtaggtcat acgaaaaata
     6841 aggtagaaga acatatttac aaaaataagt ctggggatga ggttatagta acacaatctg
     6901 gagaagattg ttattcaaaa ataagtcttg aactaagaaa agaaaatggc aagtggaata
     6961 tcataaacaa atatagtaaa gcaataaaaa tgaagtgaat ttattgatat ataaaccatt
     7021 attaac
//
LOCUS       DAEOSP010000181         7003 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.185, whole genome shotgun sequence.
ACCESSION   DAEOSP010000181 DAEOSP010000000
VERSION     DAEOSP010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7003
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.185"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            479..1393
                     /locus_tag="KRH54_002979"
     CDS             479..1393
                     /locus_tag="KRH54_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580615.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            1417..2052
                     /locus_tag="KRH54_002980"
     CDS             1417..2052
                     /locus_tag="KRH54_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBG8580616.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            2428..3144
                     /gene="pyrF"
                     /locus_tag="KRH54_002981"
     CDS             2428..3144
                     /gene="pyrF"
                     /locus_tag="KRH54_002981"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBG8580617.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            3170..4213
                     /gene="carA"
                     /locus_tag="KRH54_002982"
     CDS             3170..4213
                     /gene="carA"
                     /locus_tag="KRH54_002982"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG8580618.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            4266..>7003
                     /gene="carB"
                     /locus_tag="KRH54_002983"
     CDS             4266..>7003
                     /gene="carB"
                     /locus_tag="KRH54_002983"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBG8580619.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEV"
BASE COUNT         2922 a          723 c         1361 g         1997 t
ORIGIN      
        1 aatatttata ctttatagat tgagaaataa aaaaaatatt ttaattaaaa cagattatga
       61 ggataacaaa cttatttaag cagtatgttt tttgttgata ataatattaa agtagaaata
      121 aaaatacttt tttagtgaaa agctatacaa gaataccgaa aaaaacttat aatataataa
      181 tgagatagat atttataaat gaaaaaaaat ttttctggca aagcttaatt aaaaagttaa
      241 tagctagtgt tgttatttaa attagcatag aaaatcaaat aaaaaaatta tgctaatgta
      301 tagacattga taaggtagaa tttaagcctt ttgaatgcta tattaaacta gagtagatat
      361 tcttatgaat ttcaaaatag ggcaggagga aaaaggaaat aaaactataa aaaatataaa
      421 gtatttatat aaatttatag agtttatacg tcagaagatt tcagttagga gggcataaat
      481 ggagctttat gaagccattt tttatagaaa atctgtaaga aatttttcca gtaaaaagat
      541 aaaaaagcca ttgatggaag aaataaaaag aagttgtaaa aatttagaat acttaaatga
      601 agatttaaat attaaagcac atgttgttga tagaggtcat ataatacatt ttttgatggg
      661 taaggaatgt aaagtaaagg cacctcatta tatagtagtt acatcagaca aaggtgagga
      721 ctatttacag aatataggat ttgctacaga aggtgtagtt ttgcgcctta caacattggg
      781 aatagctact tgctggttgg aaggaaactt aaagagagaa gatatcttgg aatttgtaga
      841 tattgggaat aagaattttg aagaagatga ggaagatgat ttagattatc tagctggaat
      901 aaaaacagaa gaagatgatg aaaacaaaat agaaaatcct tatataataa tagcatttgg
      961 atatccagca aaaaatgaag agttgtttag gacgagatat gctagacctg atagaaagcc
     1021 agtaaaagat atgataaata agataaatag gaatagaaag tggaaagata tcttagaatt
     1081 gacaagaaga gctccatctg taaaaaattc tcaaccgtgg tatttccata aggatgaacg
     1141 aggtcttcat ttatttgaaa agagacctaa gaaacactgt gaagatatga ataaggttag
     1201 cttaggtgta gccttaagac attttgatat agcttgtata aaaaataaaa tagatgtttc
     1261 atatgaaaaa cttcctataa ggaataaaat aggaaaatca tattttataa ctgtagtcga
     1321 gcatgtgaaa ccagaagaag aaacccaaga agaaaatgta actctagaaa aagaagaatc
     1381 gcaagatgaa taaaattttg gaaggggata ttggatatgt ataagtgtgg aaaatgtaat
     1441 cttagaggct gttctaatgg aaatatggat aaattaatga agatatgtcc aactagagaa
     1501 attgaactac aacaaaaagc aaagcaactt tatgaagaag aagagaatag aaaaatagct
     1561 tataatgctg gattagttga agcagagggg tattgtcaat atactagact aaaagagatt
     1621 atagagtttg ctaaaaagtg tggttataaa aagattggca tagcattttg tattggacta
     1681 aaaagagaaa tgcaaacagt gcaaaaaata tttgaacatc atgggtttga agtattttct
     1741 gttatatgta aaaatggggc aatgcctaag agtacaattg gactagaagc atctcaaaca
     1801 atatcaaagt gttcagatga ggtaatgtgc aatccaatag gacaagcttt atttttaaat
     1861 gaagctaagg ttgaccttaa tgtaattctt ggattatgtg ttggtcacga tactttagcc
     1921 atgaaatata tggaagctcc tataacaact ttagttgtca aagatagagt aactgggcat
     1981 aatccagttg ctgcaatata taatgcagaa tcatattttg agaaaaaatt gtttgataaa
     2041 gatgaagaat aattcaaaga cttattgaca taaaaagcaa ttagtggttt aattataaaa
     2101 aagataaaac tttaaattat cctatagata cataaagtta tataaataaa gaaaaatatt
     2161 acagctaaat tccttaaagt agtccagaga gactaaaggg ttatagataa tgttagtata
     2221 taacctctag taaatcttct agaggttttt gatgagtata aaataaaaat ttaaatataa
     2281 taaagtcttt taaactagtc cagagagact gggaaagata tctcatagct aatgaataga
     2341 gttatgtata gataaaatcc ttatgcctct tttggtgtga ggattttttt atgattattt
     2401 aggctttatt aaaaaggaga tgtaaaaatg ataaatggta aagaaaaatt gatagttgca
     2461 attgatactg acgaatttga taaagctaaa gaactaatag acagattaga agatagtgta
     2521 gacatattta aggtagggct agagcaatac gttgccacaa aaggtaaaac aatagattat
     2581 cttaaagaaa aaggtaaaaa aatattttta gaccttaaat tccatgatat accaaatact
     2641 atgaaaagtg cagtgaaagc tgcagtgaga gataatgttt ggttaatgac aatacatgta
     2701 tcagatatgg aaggaatgag acaatgtgca ctaattgcta gggaagaagc agaaagatta
     2761 aatatagaaa agccattagt agttggagta acagttttaa cttcattaag caaccaagat
     2821 ttacaggaca taggatgtaa tatgacaaca gaagagttag ctataaagag agctaaactg
     2881 gcaaaagaat caggaataga tggggttgtt tgttcagcgc aagaagtaga taaaatagta
     2941 gaagcatgtg gaactgattt tataacagtt tgcccaggaa taagacctaa atcagcagag
     3001 gttggagacc aaaaaagagt tgtaactcca tcagatgcaa taaaaaaagg tgctcattat
     3061 ttagttgttg gaagacctat aacaaaagca gaaaatccaa gagaatcagc tattaatatt
     3121 gtaagagaaa tagaaaatgc ttagataaaa tatttgggag gtagcttaca tgaaggggaa
     3181 attaatttta gaagatggaa cagtatttga aggaaaagca tttggatatc taaaggaagc
     3241 tattggagaa gttgtattta atacttcaat ggctgggtat ggagaactat tgacagatgc
     3301 aacttattat ggacaaatag ttactatgac ttatccactt ataggaaact atggaataaa
     3361 tttagaagat gttgaatcaa ataaggtgca agtaaaagga cttattgtaa gagaaaaaag
     3421 tgataaccca aataatttta gatgtgaaat ggatatagac acatatctaa agcaaaataa
     3481 ggtaatagga cttgaaggaa tagatacaag agcattaact aaaattatta gaaacaatgg
     3541 aactaagaga ggaattataa ctttagaaaa tgtatcatta caagatgttc agtctaaatt
     3601 agaagagcat tgtaacaaag attcagtaaa aaatgttaca agaaaagaaa ttgaaaatat
     3661 aaaaggtact ggagctaaag ttgtagttat agactttggt gttaaaaata gtgtactaga
     3721 gtctcttaag gcttgtaact gtgatataac tatagtacca gcaatgacta cagtagaaga
     3781 aattttaaat ataaatcctg acttaatctt attatctaat ggcccaggaa atccagagga
     3841 tataaatgat atagttgaaa atataaaaaa attaataggc aaaaagccat tgacaggaat
     3901 aggtttagga catcaaattt tagcattggc attaggagga aagacttcaa agcttgtatt
     3961 tggacataga ggaggtaatc acccagttaa atgtttagaa actggaaagg tatttataac
     4021 ttctcaaaat catggatata atgtttcaga aatgccagaa aatacagaag taactcacat
     4081 aaatttaaat gacaatacta tagaaggaat gagacataag gaattaccaa tatatagtgt
     4141 tcagtacatt ccagaataca caacaaaaga agatttagat tgtatattta ataaattctt
     4201 agcattaata tagatttagt attatttaca gatagtaaaa accatttaga taaggagaaa
     4261 aatttatgcc taaattagat agtataaaga aaactttagt attagggtca ggaccaataa
     4321 taataggtca agctgcagag tttgactatt caggaacaca agcttgccag tctttgaaag
     4381 aggaagggat tgaagttgta ttaataaata gtaatccagc cactataatg actgataaag
     4441 aagtggcaga taaaatatat atagaaccat taacaataga atttatagaa aaaataatag
     4501 aaaaagaaag accagacagt ttacttgcag gtatgggtgg acaaacaggg ctaaacctag
     4561 cagttgaact ttatgaaaaa ggcatactag ataaatatgg agtaaaaata ataggtactt
     4621 cagtagaatc tatcaaaaaa ggtgaagata gagatatatt tagagaagta atgaaagaaa
     4681 taaatcagcc tgtaatagtt agtgatatag ttacagattt acaagcaggt cttgattatg
     4741 cacttacaat tggttatcca gtagttgtaa gaccagcata cacactaggt ggaactggtg
     4801 ggggtatagc tgataatgag gaagaattaa gagaaatact ttcacatggt ctacaattaa
     4861 gtcctgttgg acaagtatta attgaaaaga gtataaaagg ttggaaagaa atagaatatg
     4921 aggtaataag agattcaaag ggaaattgta tagttgtatg taacatggaa aatatagacc
     4981 cagtcggagt tcatacagga gacagtatag ttgttgctcc aactcaaact ttaagtaaca
     5041 aagaatgtgc aatgcttaaa aaagcatctt tagatatatt aaatgcagta gaagttcaag
     5101 gtggttgtaa cgttcaattt gcacttaatc cacatagttt tgaatatgca gttatagaga
     5161 taaatccaag agttagtagg tcatcagctt tagcatcaaa agctacagga tatccaatag
     5221 ctaaagtttc atcaaaaata gctttaggtt atactttaga tgagatagaa aatgcagtaa
     5281 ctaaaaaaac ttatgcttgt tttgaaccaa ctatagatta tgtggtggca aaaataccaa
     5341 aatggccttt tgataaattt aaaaaagcaa atagaaaatt aggaactaag atgatggcaa
     5401 ctggtgaaat tatgagtata ggaagtaatt ttgaggctgc tattcttaaa gggataagat
     5461 cacttgaaac aggaaaatat tcattagttc acacaccatc tgaggagaga agcattgagg
     5521 aattaaagaa aagagttgta gtacctgatg atgagagact atttgattta gctgaaatga
     5581 taagaagagg ctataaagta gaaatgatag aacaaattac aggtatagat aaatggttta
     5641 taaataaatt taaatggata gtagagcaag aagaaaaatt aaaagttatg aaaattgaag
     5701 atttaactaa agactatctt cttgaattaa agaaaaaggg attctctgat aaaggtatat
     5761 ctgacctaat gaagataagt ccagaaaaat tatatgaatt gagaagtcta tacaatatca
     5821 aaccagcata taaaatggtt gatacttgtg caggtgagat agatgctata tctccttact
     5881 attattcaac ttatgagcaa tatgatgagg tagttgttag tgataagaaa aaggtgatag
     5941 tattaggttc tggtccaata agaataggtc aaggtataga atttgactat tgttcagttc
     6001 actgtgtaaa atctttaaga aaaatggaca tagaaactat aatcataaac aataaccctg
     6061 aaactgtaag tacagacttt gatacatcag ataagctgta ttttgaacca ttaacagaag
     6121 aagaagtttt aagtataata gaaaaagaaa aaccagaagg tgtaatacta cagtttggtg
     6181 gacaaacagc tataaaattg gctaaatttt tacatgaaaa aaatatacct atactgggaa
     6241 ctgattttga tgatatagat gcagcagaag atagagaaaa atttgatgat ttactagaga
     6301 gactagatat aaatagacca aaaggaaagg gtgtatggtc acttaacgaa ggtattgaag
     6361 ttgctaatga gttaggttat ccagtattag ttagaccatc ttatgtactt ggtggtcaag
     6421 gtatggaaat aacttataac gaagaaaaat tatctcaata cctacaagat gcatttgata
     6481 gagaccataa aaatccagta cttatagata aatatctaac tggtagagaa atagaagtag
     6541 atgctatatg tgataaggaa gatatattga tacctggtat aatggaacat ttagaaagag
     6601 ctggagttca ctctggagat agtacaacaa tgtatccaag tcaaaatata tctgatgaaa
     6661 taaaagaaaa gatagtagaa tacactaaaa aaatagcttt agaccttaat gtgcttggta
     6721 tggttaatat acagtttata gaatttcaaa atgaattata tataattgag gttaacccta
     6781 gagctagtag aacagttccg tatataagta aagtaagtgg agtaccaata gttgacttgg
     6841 ctactaagtg tatgttagga gctaaattaa aggatttagg atatggtaca ggagtttaca
     6901 aagagccaaa attagtatca gtaaaagtac cagtattctc aatgtctaaa ttatctaaag
     6961 ttgaagtaag tctaggacct gaaatgaagt ctacaggaga agt
//
LOCUS       DAEOSP010000182         6949 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.186, whole genome shotgun sequence.
ACCESSION   DAEOSP010000182 DAEOSP010000000
VERSION     DAEOSP010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6949
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.186"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            117..1010
                     /locus_tag="KRH54_002984"
     CDS             117..1010
                     /locus_tag="KRH54_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG8580620.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            1188..2012
                     /locus_tag="KRH54_002985"
     CDS             1188..2012
                     /locus_tag="KRH54_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8580621.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEINQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            2111..2719
                     /locus_tag="KRH54_002986"
     CDS             2111..2719
                     /locus_tag="KRH54_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8580622.1"
                     /translation="MGKLLRGKIMKSDSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNIEQRNGLIDFQLDLLLVDITK"
     gene            2823..2999
                     /locus_tag="KRH54_002987"
     CDS             2823..2999
                     /locus_tag="KRH54_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580623.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            4107..4859
                     /locus_tag="KRH54_002988"
     CDS             4107..4859
                     /locus_tag="KRH54_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580624.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            4849..>6949
                     /locus_tag="KRH54_002989"
     CDS             4849..>6949
                     /locus_tag="KRH54_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8580625.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKII"
BASE COUNT         2592 a          920 c          932 g         2505 t
ORIGIN      
        1 ataggatatt attataggca ataactaaca tcaagcgata acttaataca tataaaaata
       61 aataaacaat taataaaaat taaaacttca ttataaaacg gaaggatagt gaaaaaatgc
      121 aactatataa cacccccttt aatacaaaca aacacttaat ctatttcttt acatttacgc
      181 tagtattgaa ctggatattc agtcttacac ctgcgttgct cggaattaca aatacaatac
      241 ttggagaaat cattttttat cttgcatata catcaccagc tattgtaggt ctatttttta
      301 tttctacaat ctatccacaa agtgcaaaac aagattttat ttatagatac ctaagcttta
      361 aagaaattag aataaaatgg atactattaa caatattatt ttttgcttta acttttatta
      421 cttcgttaat aatcggaaat tactttaatg taaaaacttc tgatatagat tggatacaca
      481 tagcagtgtt tagaccttct aaaatagctt atatgctatt agcaagtttt atattgagta
      541 tacttagtca agaagctgga tggagaggat atgccattga caaattatta gttagatttg
      601 gatttatagg ctcttcaatt attttagggc ttgtttgtgg aatctggtat ctagggtcgt
      661 attttacacc taatcaaata ccatataatt tggcacaata ctccctattt gatgcttttt
      721 tatttatacc aaacattgta cttttaaact ttataattaa ttttgtatat atcaatacaa
      781 gtagaagcat tttatcagct gggttagttc atatgatgta ctattttttc aacattcaat
      841 tacttctaca ctaccctatt aaattatgtg taatagctca atatgtacaa ataatatttg
      901 gtttaatatt tcttgtatat atgatttctt ctaataaatt taaacagaag gtaaattctg
      961 aaattgaaca gataaagtcg gatgattttg aatttgagtt agactggtaa ttttaatatg
     1021 aattataaat taagataatc tatgatttga tacgattact acttaaatat aatttactct
     1081 ctataattta ttgcaacatc tggaaaataa ttgtatttcc acaaacgctt gaagtatata
     1141 aaaacaaaat atatactgat tataagtgaa aaggaggtca tattttcatg tttaaaatag
     1201 gagaggtttc aaaactaaca caaatttcta ttcgtatgtt gcgttattat gatgaacttg
     1261 gtattttaaa acctgcagaa atagacaaat atacaggtca tagattatac tcagttgaac
     1321 agatttcaat actacaaaga attgtattat tgagagattc aaaatttagc gttgcagaaa
     1381 tagcaaatat cgtacataat tggaatgatg aatttgtaat taaagaattg aatagaaaaa
     1441 agaatgaaat acaaaaggaa ataaatcagg aacaacaaag aataaataaa attgataaat
     1501 ttatagaagc tataaactgt gataaagatg aaattcatta caatgttgtc tttaaaaaga
     1561 ttccaagtta taaaatcata tcacttagag agataattcc agactaccaa tctgaaggta
     1621 tactctggga aaagctatct aaatttatca aagaagaaca tatagaagta tcacgtcaat
     1681 ccaacaataa catagctttt taccatgatg aagaagtcaa agataatggt gtagatattg
     1741 aagttggaat ggttgtaaag aagattggaa agaacaaatc tggatttata tatcgtgaga
     1801 cagaagaaat tgatatgatg gcatgtacta tggtttatgg tccatatgaa aatatagctg
     1861 gtgcatatga atcgttctgt tattggttag ataaaaatag tgattatcaa atatcaggta
     1921 taaatagaca aatcggacat aaaggtgaac ataatgaaat aaatcctgaa aattatctta
     1981 cagaaattca aatccccctt ataaaagtct aatagattag tacttaaata cttaagtact
     2041 aatcacccat tgacatccca aaattttcat gatagtatct aaacgtgtgt taaacatgtt
     2101 taattttttt atggggaaac ttttaagggg aaaaattatg aaatcagatt caacaaagga
     2161 acaattaatt aatgccacaa tccagttact atcttcacat agagatgttt caagcattac
     2221 ggctagagaa attgtagcta aagcaaatgt caatttagct atgattaatt attattttaa
     2281 atctaaagag gagttaatta atatttctat taaaaaattt ttaaagcgtc atatggatga
     2341 atataaggct acacatgata ctaaaaattt agcacctaaa cagcaattaa gggaaatact
     2401 aattgcattt tttgatttta taggtgaaca tagtcaattt ataaagattt ctgtacccta
     2461 tatattgata caggaagaaa ttgtttatcc atttgagata ctacctttaa ttaaggctca
     2521 ctatgaaaat cagaataaca aaaaaactga agctgaatac agaactattg cgtatcaaat
     2581 aacttcattt attcagttgc tttttcttcg ttctaatgct tttttcaagt atagtggtat
     2641 tgatattctt aatattgaac agagaaatgg tttaatagat tttcaattag atttattatt
     2701 ggtagatatt acaaaataat actatattaa ttttttatgt acattttttt attttatggt
     2761 aacgtttgaa aatattctca ttgactaaaa atacttgcat taaataaggg ggaggaattt
     2821 taatgttttt tacatttata ggagtaatat cagcaatttt attgttgtgt acaattatat
     2881 gtggtttatg gataaaactt aataatcgta ataatgactc taattctcat tttacttttt
     2941 caatactttc aattgctttt tcactgttaa ttgtggtggc ctttttagta agaaattaat
     3001 gtctttgtta tgctgaggtt tattattata gatttttatt attgcatgat ttttactata
     3061 ttatagtatt tttattatat tacagggtct tatttattat agtgttttgt tgttacagca
     3121 ttttattata ttacagagtc ctatttatta taggattttt attatattgt aaaatcttat
     3181 ttattatagg attttgttat attacaatct cttatttatt gcagtgtttc tattatattg
     3241 caaactctta tttattatgg tgttttgtta ttgcaacatt tctattatat tgtaaagtct
     3301 tatttattac agttctttat tacactacag acttttatta ttacaatctc ttatttatta
     3361 cagttcttta ttatactaca gacttttatt attacagcat ttctgttaca ttgcaaagtc
     3421 ttatttatta cagttcttta ttatactaca aacctttatt attacaacat tttattattt
     3481 taacttcttt actataatat agtaatattt taatatctca aaataatatc aatcactttt
     3541 cagcatagtc taaaatactt ccactttcta aaattatcta tcatacctat actaataata
     3601 cttttcaacc tttttattat aaatttcaat tgtttttaac aacctattta tttccaaaac
     3661 tccaatatat tttctcaatt acattgtgtt aataacccct aaatcctttc aaattattta
     3721 caatacattc cattaaataa tcttactgct ctataattct ttttttcttt ttaaccctac
     3781 tatacattat ttacatcatt tttttcaata caacattcgt tattctataa cctttttact
     3841 tttactatat accttttttc taactacacc ttcaatgttc cacatcatat tactttacca
     3901 caacaattaa tacctaaaaa taaccctcta aatcctccaa taatataatc aatttaacta
     3961 taataataac ctctcacact atccaaacaa tatattttca aacattacaa aaaaacatcc
     4021 aactattaca atatagctca tatccctcct cccctttcaa atgctatcat tgacttacaa
     4081 gataaatgaa agtgaggatg atatatatgc ttaaagtaaa ccaattatgt aaatcatata
     4141 aaacaggtag taaaacatat cctgtattaa aaaatatttc ttttgaaatc aacaaaggag
     4201 aatttgtcgc aatcatgggt tcatcaggaa gtggaaaaac tactctttta aattgtattt
     4261 catgttttat cccaattgat aatggggaaa taattcttgg aaataataat ataagaaatt
     4321 taaaagaaaa ggaactttca gaagttagaa accaacaact aggatttgta ttccaagatt
     4381 tcatgctatt tgatggtctt agtgtctttg aaaatatatg tgttccacaa attatcaaag
     4441 gtagtcaagt cttacaaatg gaaaaccgtg caattaatct atgtgagcta tttggtattg
     4501 aacatatcag aaaaaaatac cctgccgaaa tatctggtgg agaaaaacag cgtacagcag
     4561 tagcaagagc actcatgaac aacccagatt taatattagc agatgaacca actggtaacc
     4621 ttgatagtaa gtcttgtcgt acagtcattg aatcattttt aaaagcaaag caagaattag
     4681 atgctactat actcatggtt acacacgata gctttgcagc aagcttctgt gaccgagtta
     4741 ttgtgcttaa agatggaata atttataaag aagttgtaaa atcaaattca agacataaat
     4801 tcctagatga attactagaa gttttaaaag aaatgggagg cgctcaagat gacaattaat
     4861 aaattataca gtaaacttcg agcctataat tggaagaact atacaacttt attcttttgt
     4921 ataatacttt ctgtggtatt agtaacttca tatgctctaa tttattttag cccaacaata
     4981 caaaacatac ttccaactgg tggagatagc agaaaacagg ctacaatgat attctcagtt
     5041 gctatattag gatgcgctgt ctttactaca tacgcttctt ccctcttttt aaaatttaaa
     5101 agcaaggaaa ttggtatttt catggctcta ggaacagata agaagaaatt aaaaaaactt
     5161 ctattttcag agatattgtt aatttcaata gtatgctgtt ttttaggtct tatattaagt
     5221 attcctgtat cttttggtat atggaaaata tttcaattag tgattattga taccagagaa
     5281 atgacatatc attttggaac tcaaggattc atatatggat ttatatttag tgtatttgtg
     5341 acattatgca tatttatact ttctgtaaaa tttattaatc gtacaaatat aattgatata
     5401 ttaaatgacc agagaatatc tgaaccaata caggatgtaa aggactggta tggtatatta
     5461 ggtataattt ttattatact cggtattttt ctaggatatc tcgtaccttc actaactata
     5521 aacaaattaa attatagact accatctgta tggagcttta cttatctact ctctcttatt
     5581 ggaatatata tgattatgac atacatagtt gtacatgcta aaagaggaaa acatccagag
     5641 aaatactata aaaacatcat atcaactagc atgatgagat ttatgggaag acaaacagta
     5701 aaaaacatgt gtgttatttc atttttgatt gcaggagcat tatttgctgt attctatgtt
     5761 cctaatatgg ttacaggtat ctatgatact cttaaaaata accctataga ttatactttt
     5821 acctacaatc aaagagataa acaggtatca aaaaaagata ttgaacaatt agcagataaa
     5881 cacaatataa aaattcaaaa ctatatggaa attccttcta tagaattaat tgttgatggt
     5941 gtagaatcaa tatatcataa agatggaact atcgaaaagg tatatatgaa aaaaagctcc
     6001 tatgatatgt tctttaattc taaagatttt tccaagctaa ttggcaaacc aataactata
     6061 aaaaatggtg aatatcttgc tgttgtaaat aaaggaaatg aaaatcaaga ttataaatat
     6121 accaaattta tatcaagccc agtcacagaa aaagaaatcc cctttagata tgctggaaaa
     6181 ataatattta atggttcttt ttttcaaaat agaagtatga acgcctatgt ccttaatgat
     6241 gaggattata aatcatttac taaagattta tccataaaaa attacaataa ttctatacta
     6301 tttaatgtag ataaagatac ctatgctttt gccaatgact taaagaaaga aattataaaa
     6361 agaacatcta aggaaatggg catgttcagt ggttatgatg aatacgtaaa aaaacgttat
     6421 gaatcaactg gaagaaaata ttttatggat gaagaatatc caagtggtga aggtcattta
     6481 aaactagacc caaacaacaa tcagttgtat cttaattgga aatactatcc taactttaaa
     6541 attttacaaa gccaagatat gataaaaaac atggcagtct tcttaatgtt attcatatat
     6601 gtggcaatca tatgttttac agcagtagga atcattgcat atacaagggg tgttactata
     6661 gctataaatc acaagcaggt ctttatggat ttaaatcgat taggagcaaa caatgattat
     6721 ataaagttct gtattagaaa tcaattaaag aaagtatttt cataccctct tattgcagga
     6781 agttttttaa tttatctgtt taattttatg attatgttaa ataatgatgg tagaatcgtc
     6841 aatagtgagg tcaatgctct tttgataaac ttaggtttta tagggatttg tggggtttat
     6901 atgtacctta tatatctatt aacttataag aaattcaaaa aaattattg
//
LOCUS       DAEOSP010000183         6938 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.187, whole genome shotgun sequence.
ACCESSION   DAEOSP010000183 DAEOSP010000000
VERSION     DAEOSP010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.187"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            52..525
                     /locus_tag="KRH54_002990"
     CDS             52..525
                     /locus_tag="KRH54_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:184..186,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBG8580626.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            557..1867
                     /gene="grdB"
                     /locus_tag="KRH54_002991"
     CDS             557..1867
                     /gene="grdB"
                     /locus_tag="KRH54_002991"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1604..1606,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBG8580627.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            2123..3655
                     /locus_tag="KRH54_002992"
     CDS             2123..3655
                     /locus_tag="KRH54_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBG8580628.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            3666..4811
                     /locus_tag="KRH54_002993"
     CDS             3666..4811
                     /locus_tag="KRH54_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBG8580629.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            5121..6200
                     /locus_tag="KRH54_002994"
     CDS             5121..6200
                     /locus_tag="KRH54_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBG8580630.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            6313..6912
                     /locus_tag="KRH54_002995"
     CDS             6313..6912
                     /locus_tag="KRH54_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580631.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
BASE COUNT         2684 a          798 c         1399 g         2057 t
ORIGIN      
        1 agtgttatag atatataaaa atatattaga aatataataa ggaggattat tatgagttta
       61 cttagtaata aaaaggttct tataataggt gaccgtgatg gtataccagg acctgcgata
      121 gaagaatgtg taaaaacagt agaaggagca gaggttgttt tctcatctac agaatgcttt
      181 gtctgaacag ctgctggggc tatggactta gaaaatcaaa acagagttaa agatgctgct
      241 gataaattcg gagctgaaaa tgttgtgatt ttactaggtg ctgctgaagc cgaagctgca
      301 ggtcttgcag ccgaaacagt aactgctgga gatccaactt tcgctggacc acttgctgga
      361 gttgccttag gattaagtgt ttaccacgtt gttgaggaac caataaaatc attatttgat
      421 gaaagtgtat atgaggacca aataagtatg atggaaatgg ttttagaagt tgaagaaata
      481 gaagaagaaa tgtctggtat aagagaagaa ttttgtaaat tttaatgcct aaaagcaaaa
      541 ggggtgaaac taagagatgg gaaaactaaa agcagtacat tatattaatc agttctttgc
      601 tggtataggt ggagaagaaa aagctgatac taagccacat gtggcagaaa ctttacctcc
      661 aataagttta caacttgata aattattagg tgaagatatt gaaatagttg gaacagtagt
      721 ttgtggagat agctatttca atgaaaatat agatagtgca agtgaagaag ttttatcaat
      781 ggtaaaaggt tttgaaccac aacttttcat agctggtcct gcatttaatg ctggtagata
      841 tggggttgct gctggaacaa taactaaagt tgtaaaagat gctttgaata taccagcttt
      901 aacaggtatg tatgttgaaa atccaggagc agatatgttc aaaaaggatg tttatgtagt
      961 agaaacttct gattctgcag caggtatgag aaaggcactt cctaagatag ctaaacttgc
     1021 agtaaaactt gctaatggag aagaaattgg aactcctaaa gatgaaggat acatagctag
     1081 aggaataaga gttaactact tccatgaaga cagaggttct aagagagctg ttgacatgtt
     1141 agttaagaaa ataaaaggtg aaccatttga aactgaatac ccaatgccaa actttgatag
     1201 agttgaccca agtaaagctg taaaagattt atcaaaatgt aaaatagctt tagttacttc
     1261 tggtggtata gttccaaaag gaaatccaga tagaatagag tcttcttcag cttctaaata
     1321 tggaacttat tctatagcag gagttatgga tttaacagaa gagacttatg aaactgcaca
     1381 tggtggatat gacccagtgt atgcaaattt agatgcagat agagtattac ctgttgatgt
     1441 tcttagagat ttagaaaaag aaggtgtaat tggaaaatta catgagactt tctatacaac
     1501 tgtaggtaat ggtacatctg ttgcaaactc taaaaaatat gcttctgaaa ttggagcagc
     1561 attagtggct gatggtgtag atgcagtaat attaacttct acatgaggta cttgtactcg
     1621 ttgcggtgca acgatggtaa aagaaataga aaaaactgga cttccagtag ttcatatgtg
     1681 tacagtagta cctatttcat taacagtagg ggctaataga atagttccaa ctatagcaat
     1741 acctcatcca cttggaaatc cagcgcttga ccctacagaa gaaaaagccc taagaagagg
     1801 ccttgtagaa aaagcattaa atgctttaac tacagaagta gatggtcaaa cagtatttga
     1861 aaaataaaaa taacacacac atatattata tagataaaaa tataaacaca cactaaaaat
     1921 aaaaaataac tatacacaaa aaaataatat agatttcatg aatggataaa ttggagttat
     1981 tcattcatga aatcttaaaa aatgccaaaa tacataaaca ataatagaat caaatataca
     2041 ataaaataca atgattagag cttttaataa gtttaataag attaaaaaat aagtgttcat
     2101 aaaatttatg gaggtgcaca aaatgactta tccagtttta aaaggggcag gctatgtact
     2161 tattcatact ccagatatga tagtgcaaaa tggaagtact tgtacagttg aaagagcaac
     2221 aaatccagat tcagaatttt taaaagaagt aagtaatcat ataagaagtt atgaagatgt
     2281 agttaactac atgccaaatc aagtttatat aggaaataga agaccagaag aattaagaga
     2341 tttaccaatg ccatggtgtg aacaaaagat tgagggaact agaaatggta agtttggaga
     2401 aataatgcct caagatgagt tcatagcttt aatgcaaata agtgatgcat ttgatttagt
     2461 aaaattatca caagaattta ttgatgaagt aaagccaaaa atcgaaaata actaccctga
     2521 aattgcacca tttgtaggta aattaaaggg tgatgatata gaagaaggta aagaattagt
     2581 agcaacacac atagctgaag gattatacca tgatggaaaa tttgttggtt atgtaaaaag
     2641 agcacatgat gtagatgtta acttaaatgc ccatacaatg tttgaaaatc tagtagtaaa
     2701 agcatctgga gttttatctg ctattcaaat gctaagacat agcaaaatag accctgctga
     2761 aatagattat gttattgagt gttcagaaga agcttgtgga gatataaacc aaagaggtgg
     2821 aggaaacttt gctaaatcaa tagctgagat agcaggactt caaaatgcaa ctggttctga
     2881 cacaagagga ttttgtgcag caccaactca tgcattaatt caagcagcag ctttagtaaa
     2941 agctggcata cataaaaatg taatggtagt agcaggtgga gcaagtgcta aacttggaat
     3001 gaatgctaaa gaccatgtta aaaaaggtct tcctgtactt gaagatgttg ttggaggatt
     3061 tgcagtactt gtatctgaaa atgatggtgt aaatccagtt ataagaactg atttaacagg
     3121 taagcatact gttggaactg gttcttcacc acaagcagtt atgacagcac ttataacttc
     3181 aggtcttgat agagctaatt taaaaataac agatgttgat gtgtattcag ttgaaatgca
     3241 aaacccagat ataactaagc cagcaggagc aggagatgtt ccagaagcaa actacaaaat
     3301 gataggggca cttgctgtta aacgtggaga cttagaaaag aaagaattga aagattttgt
     3361 ttctaacaaa ggattaccag gatgggcacc aactcaagga catataccat caggagctcc
     3421 ttatatagga tttttaatag atgatttaac tacaggaaat agaaatagag ctatgatagt
     3481 tggaaaaggt agtttattct taggaagaat gactaactta tttgatggag tatcttttat
     3541 agctgaaaga aatacaggtg ttactgaaga gacttcagga atatctaagg atgaaattaa
     3601 gaagataata gctgaatcta tgaaaaaact agcattagat atgttagaag aataggggtg
     3661 aggatatgtc aaaaaaagta atcgcagatg tattcttaga agtagctaat gcaatagaaa
     3721 gcggcgaatt tggcaaaaaa gtaaaaattg gtgttactac acttggaagt gaacatggtg
     3781 tagaaaacat ggttaatggt gcacaattag caaaatctaa tttatttgat attgtgctta
     3841 ttggaccaaa agtggaaact gaccttgaag tagttgaagt taatgatgaa aaagaaatgc
     3901 atgcaaaaat ggaagaatta ttagattcag gatatattga tgcttgtgtt actatgcatt
     3961 ataattttcc aataggtgta tctacagtag gtagagtaat aactccagca aaaggaaaag
     4021 aaatgatact tgcaacaaca acaggaacaa gtgctactaa tagaatagag gcaatggtaa
     4081 gaaatgccat ttatggtatt gctacagcta aatcaatggg taacaaatgc ccaaaagttg
     4141 gaatacttaa tgttgatgga gcaagacaag ttgagaagtg cttaaaagaa ttaaaagata
     4201 atggatatga catggagttt gcggattcaa ttagagcaga tggtggatgt gtaatgagag
     4261 gtaatgattt acttgtaggt gcacctgatg ttatggttac tgatacatta tctggaaata
     4321 tatttatgaa agtattttct tcatatacta caggtggaga ttatgaagct caaggctttg
     4381 gatacggacc tggagttgga gaggactatg atagaaaggt acttatagta tcaagagcat
     4441 caggttctcc ggtagtagca aatgctttaa aatacgctta tgatgttgta aagggagata
     4501 taagtaatgt tgcaagaaat gaatttgcaa aggttaaaaa agcaaaattt gatgacatta
     4561 tatcctctct tactaaaaaa gaagttaaag ctgaaaaagt agaagttaaa atgccagata
     4621 aagaaattgt tactcgtcaa atagctggtg ttgatataat ggatttagaa gatgcagtat
     4681 ctgagttatg gaaaaacgga atctatgctg aaagtggtat gggttgtaca ggtccaatag
     4741 ttttagtaaa tgaagctaaa ggagacttag cagttgaaac tttagttaaa gctggatata
     4801 ctgcaaaata agtacatttt ttatatgtaa ataagaacat atagttataa ttttattgat
     4861 tataattata tgttcttttt attttatatc ttgtcaatat ttatatatta acacaatcca
     4921 tttatttgat tctctagcta atttattata tttattcagt taaaattaag ctttctaact
     4981 aaataaaaac taatgaagta ttaaaatgaa attgataaaa tatgaaaaaa ttttaggaat
     5041 aaagtatctg ttagataaaa ataagattaa ttagtgatat aatataatta agataaaatt
     5101 tattatttgg aggcaaaatc atgagcagag taaaaaatgt agtagaattg ttggaaacta
     5161 aaggtgttga tgcactatat ctaacaaaga aaactaatgt aaactatata agtggttttc
     5221 ctgatgaaga agcatatgca gtgatttgca aagatggaaa ctttttagtt actgatagta
     5281 gatatatgga gcttgcagaa aaggtttgca aagattttga aattataaat tggcataatt
     5341 ttgatagaag tgtggcaaag gcagttaaaa gtgtatgtga taaagtgggt ataaagaaac
     5401 ttgggtttga aagaactaat atagtgtttg ataaatatga agaacttaaa aatctgattg
     5461 agaaggataa tggagaatta atacctacag aaaatattgt agagactttg agatatgtaa
     5521 aagacaaaga tgaaatcaaa aatacaagaa aagcttgtga aatagcggat aaggcattag
     5581 aagaattgat tccacatata aaagctggag ttagcgaaat tgaactggct acaaagttag
     5641 agtactttat gaaaatgaac ggagcacaaa atatagggtt tgaaactata ctgatatcag
     5701 gagcaaagac ttcattgctt catggtaagc ctagtgataa gattatagaa aaaggtgatt
     5761 ttgtacttat agactatggc gctatgtata atggctatat atctgataca actagaactt
     5821 ttatagttgg aggagcttct gaaaagcaat tagaaatata taatttagtt aaggaagctc
     5881 aaaatgtagg ggttgagaac atgaaagctg gtgtacatgc gacaattcca gatgctgaga
     5941 taagaaaagt tgtcaaaaaa tatgaagact attactatca aggtatagga catggagtcg
     6001 gtagagatgt tcatgaagaa cctttcattg gtaattatgg tgataaaatc atagaagagg
     6061 gatgtataat aacaatggaa ccaggtattt atttccctgg atggggtgga gttagaatag
     6121 aagatactgt tttgattact aaaaatggtc cagaaagact tactaaattc cctaaagatt
     6181 tgatgatttt agacaaataa tttaatttat atatattttt ttacataaaa tataacataa
     6241 tctcactatt aagctctatt tacatattat agtaaagagg gcttaatttt attgtgagag
     6301 gtgatagtat atatgtataa aaaacataaa tttataaaaa atataaaaaa agcctggtat
     6361 aaaatcaaat taattgatag atgccttata attgtaatgg caatattaat gtttcaatct
     6421 atatataatt tatttgttaa tgaagtaaat tcacaagata ctacaactat tgatgtagta
     6481 gtaagaacca cttcagcagc tatatttggg tattttttaa gtgctaattt tataaagaga
     6541 cctttaagga agtatacaaa tactgatgta tcttcaaatc tttttataga taataattta
     6601 tccccaaaag gtcaagagtc taatcaaaat aatataatga atgtcaaaaa taccattgga
     6661 tttacttcag aagctgataa ttatgaaaag aaaataccta ttaataataa tgaaggtttt
     6721 gaagaaggag aaactagtga attgcaaatt attatagcta ctgtaatttg tatagtagca
     6781 ttaacagtac tttttgttgt aagaaatttt acaactacaa catcagcttc tttaggtact
     6841 atttcacaga tgagagattt tgtctcagga tgcgtgggtt ttttgctagg ttgtccaagt
     6901 aaacctgatt aaaaaagtat tatcaataaa aggtattt
//
LOCUS       DAEOSP010000184         6809 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.188, whole genome shotgun sequence.
ACCESSION   DAEOSP010000184 DAEOSP010000000
VERSION     DAEOSP010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6809
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.188"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            18..590
                     /locus_tag="KRH54_002996"
     CDS             18..590
                     /locus_tag="KRH54_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HBG8580632.1"
                     /translation="MKKLVKILINIICIITLIFSSLSIYIKLSEYKKADEVYTELREN
                     TINNSKYQELYDKNNDYRFWLKINNVNIDYPVVQGYNNDFYLTHDFYKNYLPLGSIFM
                     DYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEVFFKENNLVEINYKVQTYTYEIFS
                     VYTADLINRDYLSIHFNNNDEFKYSLNYIT"
     gene            complement(1603..2265)
                     /locus_tag="KRH54_002997"
     CDS             complement(1603..2265)
                     /locus_tag="KRH54_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580633.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(2282..4837)
                     /locus_tag="KRH54_002998"
     CDS             complement(2282..4837)
                     /locus_tag="KRH54_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBG8580634.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDEKVSKGNSVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNRLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPHDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKNDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLFRLMGAKKRNVRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEVLYSEAGLNPKLYINNEIVIKAILVGIISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDRLVSKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDDYIDEKLNDFVEEGSLD
                     DIKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMK
                     VRVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLENPYDEIVNN
                     EIQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNL
                     LIRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAY
                     INYGIENFMSFTFPIKESLLFFAISVFVCLIAVVVSNRDFESMNIIEGIKENE"
     gene            complement(5070..5264)
                     /locus_tag="KRH54_002999"
     CDS             complement(5070..5264)
                     /locus_tag="KRH54_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBG8580635.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSGDDIERITKEKYTKEN"
     gene            complement(5276..5455)
                     /locus_tag="KRH54_003000"
     CDS             complement(5276..5455)
                     /locus_tag="KRH54_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580636.1"
                     /translation="MIHVEISEKEFFRKIEEVFDKGEGNIEVDNLGDIKIQVKKFDDK
                     TKSLELKLIKEFKLK"
     gene            complement(5460..5717)
                     /locus_tag="KRH54_003001"
     CDS             complement(5460..5717)
                     /locus_tag="KRH54_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580637.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLGNLEYTKEQPSQVLH
                     YSMTKDKKGTKEILSIQCVKDFSYNEKLSEDDKFIMAIYNI"
     gene            complement(5977..6809)
                     /locus_tag="KRH54_003002"
                     /pseudo
     CDS             complement(5977..6809)
                     /locus_tag="KRH54_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
BASE COUNT         2307 a         1039 c          693 g         2770 t
ORIGIN      
        1 atacaagggg taattaattg aaaaaactag taaaaatatt aataaatatt atttgcatta
       61 ttacgctaat tttctctagc ctttcgattt acataaaatt atctgaatat aaaaaagctg
      121 atgaagtata tacggaactc agagaaaaca ctatcaataa tagcaaatat caagaacttt
      181 atgataaaaa taatgattat agattctggc ttaaaataaa caacgtaaat atagactatc
      241 ctgtagtaca aggatataac aatgatttct acctaactca tgatttttat aaaaattatc
      301 ttcctttagg tagtattttc atggattata gaaataattt tgaaaatgat aagagtttaa
      361 tagtctatgg tcattatatg aaaaataaaa ctatgtttgg tcagctagaa aactatactg
      421 atgaagtatt tttcaaagaa aataatttag tagaaataaa ttataaagta caaacttata
      481 cttatgaaat tttttcagta tatactgctg acttaattaa cagagattat ttaagtatac
      541 actttaataa taatgatgag tttaaatatt ctttgaatta tataacctaa aaatcagtac
      601 acaaatctga tataaaggta gatatttcag ataaaattat aactttgtat acttgtaaat
      661 atgaatttaa agatgctctt actatagttc atgctaaatt aatttcaaaa aagagaccta
      721 taagtaggtt tgtgtaaatg cagacttatc tacagtcttt tttgaacgta aaattggtaa
      781 gtatagaata atattcatat taaacttttg taggcaaaga aatgaaaaat caaagagaaa
      841 taattattac agatttagat taccaagctg aattaagaaa gtgtaaaact atggaggatg
      901 tagttagtga agataatata taatagttta acttataaat tatcttggtg atattaatga
      961 agtttcaaag tatatattat ttattgatat aaataaaatt aaatctgttg ctaatagatg
     1021 tacctttgtt tggagacaaa ctattgaaga aatataacta aaatgttgaa gtagctacct
     1081 gactttattt tgaaatgcga acaagatttt ggcattaaga taatttagaa agaatcactg
     1141 gtacacaagg atgtgaactt agaatgaata gaagtattca ggcataagga gctttcaccc
     1201 aaataaaaca tagtatgaac tttaaaagaa ttctaatcag aggcaatcaa gatattcttt
     1261 cagagtttat acctgtagtg ttagcctata gtatttctaa gtttcacaaa taagataatc
     1321 attaataaat gtagtatgta tattcatcct ctcaaaaata ttttttaatc ttacactttg
     1381 taaattattc tattttaaaa tggtattttt atacctttat ttcttatgac cgttttcaag
     1441 ctcaaatttt gcataaatat aaattaatga aaagcaagta aaaaaagaac tgtcgcacag
     1501 caatttaaaa ttactcattc gacagtcctt atttcgtgga tttaaatagt ggagaagaat
     1561 cacaagaata atacccacga ttaaaccttt atacaataca ttttatatga ctttaccatc
     1621 actaatagtt attatacaat cagccatctt agctatttca tcattatgag ttatcataac
     1681 caacgtctgc ttgtattcat ttacacaata ctttagtaaa ttcatcacct cattagttgt
     1741 tttactgtct aaatttccag taggttcatc tgcaaaaatt attgatggct tattggcaag
     1801 tgatcttgct attgcaactc tttgttgttg accacctgat aattcattag gaaatttatt
     1861 aatctgactt tctaatccta acttagttat taaatcatta atataattat tatctatctt
     1921 tcctttagca attaaaatag gaagaactat attatcataa acattgataa caggtattag
     1981 attatagttt tggaatataa acccaaattc tttacttcta agattagata gcttttcatc
     2041 acttaaagta tataaatcta aattatccat aaatacatta cctaaagttg gtttatcaag
     2101 ccctgccata cagtgaagta atgtgctttt tccagaacca ctttgtccaa ttattgcact
     2161 aaatttatgg ggttctatct ctaaagtaat tccatcaaca gcctttattt cattttctcc
     2221 ttttccatat atttttttaa tatctataac ttttaacatc ttcatacatt tacctcccaa
     2281 attattcatt ctcttttata ccttcaataa tattcatact ttcaaaatct ctattactta
     2341 caactactgc tattaaacat acaaatacag atatagcaaa gaatagtaaa gattccttaa
     2401 ttgggaatgt aaaagacata aaattctcta ttccatagtt tatatacgca tcattattcc
     2461 aatttgtaaa tttaactaga tttataaatg atggaatcat agctattata aagctaaaaa
     2521 ctgcatatgc taatgcttca tatataagca tttttttcat atttttaaga ctaacaccta
     2581 atgctctaag cgttgaaatt tctttttttc taattagtag atttgttctt atagtacaga
     2641 aaatattaat tgatgctatt attaaaacta gtgcaattat tgctatttgt gatttaatgt
     2701 aacttttata tatactttct gacatttcat gataacttaa tctactatta aatagagacc
     2761 ctggtatact tcttgaaatt ttctgtatct cattattaac aatctcatca tatggatttt
     2821 ctaaatttat gcctaattga gtgtattttt gttctcctgt tatgttttta gagtgaatat
     2881 tacttgttat aatttctaga tctggcataa aatcgccatc tcccatagca atccattcat
     2941 cctttaaaat tacagccact cttaccttca tctctttata aacaattgta ttattttctt
     3001 caacaggaat ttttacagtt attatgtctc ctatatttaa ttctttgaat ataggcttta
     3061 tagtatgatc atcaactata tcataaaaat aattatatac tgctacattt ggatatccat
     3121 cagtgtcctt tttaatatca tctaagctac cttcttcaac aaagtcattt aacttttcat
     3181 ctatataatc atcatcataa agctttaaaa ctaatttatc ttccattatc tctttttttt
     3241 ctactccatt ataggttgta aattctttgt ttatatcctt attattaaaa gtaaacattc
     3301 catttgttat ttgaatttta tgtatacttt taacaccctt aattttttct atattctcta
     3361 catcactatc cgtgtatttt gatatttgcg ggtctgcatt tactccaaat tgcattgtta
     3421 tattatatct attttgcata tcttcaattt tactgttgac ctcttcttgc atacttgaga
     3481 atgtagtaat gaatatatat cctcctaaag caatagacaa catagatata gcagctctaa
     3541 ccttatttct tccaatattt ttaagtccca taaatccata aaatccaaat attttgctta
     3601 ctagtctgtc tattccaagt ctacttctgt gatttttaat tttgtctgta tttattattg
     3661 attcaatagg tgatatcttg ccgattttaa aagttggtat aatacaagat ataaaaacag
     3721 aaattattcc aaccaatata gcttttataa ctatttcatt atttatatat aatttagggt
     3781 taagaccagc ttcactgtat aaaacttcgt tataattact aattcctata tatgaatatg
     3841 taaccccagc tactaaccca agtagtatac ctattattgt tataattaat gcttgataga
     3901 ttatcataca ccttacgttt cttttctttg cacccatcag tctaaacagt cccatttctt
     3961 ttactgattg atttagtgta atattgaaaa tattaaatat gaaaattata gatactgtag
     4021 ttattaaaac atttctttta aaaacattcg gatcattctt atcatcttct acataacgaa
     4081 gttcccttac aagaggtaca tttggtagaa aacttttttg tcctaactta tattttttca
     4141 taagataatc acattcacct tcaatatttc tccatccact tttaaaactt aatatactat
     4201 tataactaat cgcatcatga ggtataatgt tatttgtttc atcattgcca taagttactg
     4261 cttcgtattg ccaaaccaca tcatagtacc cttttggttt ttccataact cctactattt
     4321 taaattcttt atttatagaa aataatcgat tacttccgtt agtatcttta tagttctttt
     4381 ttattgttaa atttaatgta gatcctactc cttcacttaa attcattgct tctagtgctt
     4441 ttttctctaa tactatttca ttgttgtttt taggtagtct accttctatt attttgttgt
     4501 atctactttt tagatactct tcgtttgatg aagttaatat aaatgagttt ccttttttat
     4561 ctgaaatacg tcctaaattt tgtaccgaat taccctttga tactttttca tcagtttcta
     4621 attttttagc atcatcaagt gtcaaatcac taaactctac atggtatcca ctgtaaagtt
     4681 catgtattgc tactacattg ttttcatttt gtgaatcttt tataacacta ggtccaaata
     4741 tcaacataac acctaacata actcctatta ttaaaagtag tgttctaagt ttatttttct
     4801 ttaaatatgc tagtgacatt tttaagtaaa tccccatata atcctcctat tttactataa
     4861 attatttatc caactcatct gttaagtaga ttggtggata ttttaaatct tttaattctt
     4921 cattattttt tgaagcttac taccatcttc ctcatgataa acttgcagta tcaccttatt
     4981 tacatgtagt gcataataat caccactata aaaatattgg tattcataac ctggataatt
     5041 tttgtaaaat gatttataaa gtacccatcc taattctctt ttgtatactt ttcttttgta
     5101 atacgctcta tatcatcacc tgaaactcct tgatatgtca tatatttatc tatatatttt
     5161 tcagcttcat tccattttct aaaatagttg ttgtatataa taaatcctat tatggctata
     5221 atgcttataa ttaaagttaa taaaatagct ttgtattttc tcatagtgaa tcttactact
     5281 tcaatttaaa ttctttaata agttttaatt ctaaagactt agttttatca tcaaatttct
     5341 taacttgaat ttttatatct cccaaattat caacctctat atttccttca cctttatcaa
     5401 atacttcttc aatttttcta aaaaattctt tctcacttat ttctacatga atcaatgatc
     5461 tatatgttat aaatagccat tatgaattta tcatcttctg acagtttttc attataagaa
     5521 aaatctttaa cacattgaat tgataatatt tcctttgtcc ccttcttgtc cttagtcatt
     5581 gaataatgta aaacttggct aggttgttct tttgtatact ccaagtttcc caaagacaaa
     5641 acaaaacttc catctgattt attttcagct accctaagac cactattttc tattgattct
     5701 gatattttct ttgacaagtc ttcttgatac tagaaatcgg ttcaatagta tatatttttt
     5761 catttgtatc tgaaacctta tttgtgcatc ctgttattaa acctagcgtt aagcttatag
     5821 ctataaccat agttattgta atttttttat cttcatataa tcccccttat atattgaaaa
     5881 ctcgttattt ctcataaaaa aaagacgcta tctttttcta ttaaaagata acatccctat
     5941 atgtttagta tcaatatatg ttccccaact ggtaggttaa tcgacccaac tatcaaaatt
     6001 ttgatttaat ggtatataaa tattcaatat aaatttgtct tttttatctt caaatgataa
     6061 atctcctcta tattttttta tagaagattt gatgctttaa atccctattc catgtacaag
     6121 tttatctttt ttatctgtta ataataaact tttattcttt ctaattgaat ttattttact
     6181 attctcacat ttaattacga aataggactt tgagatagtt ccttttattt gtatatattt
     6241 atcaatactt ttatcatgta tcttatcgca agcttctatt gcattatcta agtatttgca
     6301 aatatattag atacatctat tggttttata aaattcactt tggaaaaatt gatatcacaa
     6361 atgaatttaa tttttctttt ttcacttaca tcactttttt catttattat tatatccaat
     6421 ataacattac ctgtattgta aattgattta aattctttaa tttaatcttt taaactattt
     6481 acatacccat caattttcct tttttatatt tgagattttc tatacaatat atatgattat
     6541 ttatatcatg ataaagcttc ttaactttag cgtgtgattc ttgaatactt agataatgag
     6601 aatactgcat atctaactta ttttttatta cttcattttg caattttaac ttattttcct
     6661 taattatttt tataaacact ataataatga ctaagctact agatagtatt aacctaggta
     6721 tcacaatata cccaactata tatatttcct aatacccacc aaattgatac atttttctat
     6781 ttatataaat tattaatata gtaaataaa
//
LOCUS       DAEOSP010000185         6780 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.189, whole genome shotgun sequence.
ACCESSION   DAEOSP010000185 DAEOSP010000000
VERSION     DAEOSP010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.189"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(669..1298)
                     /locus_tag="KRH54_003003"
     CDS             complement(669..1298)
                     /locus_tag="KRH54_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBG8580638.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALRQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(1377..2339)
                     /locus_tag="KRH54_003004"
     CDS             complement(1377..2339)
                     /locus_tag="KRH54_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBG8580639.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKVKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVIEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(3101..6697)
                     /gene="cbpA"
                     /locus_tag="KRH54_003005"
     CDS             complement(3101..6697)
                     /gene="cbpA"
                     /locus_tag="KRH54_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HBG8580640.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     AEGDTNAGNAGGDANAGNAEGDANAGNAGGDTNVGNPEGDANAGNAGGDANAGNAEED
                     ANAGNAEEDANAGNAEGDANAGNAEEDSNAGNAEEDSNAGNAEGDMNALVVEEATAED
                     PTSVGDFKISGGEINVDFKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAEL
                     IFDGVNIKTASNSPFTLTPDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLI
                     IDDSVVNRDIEGEFIVSEGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYY
                     SAGIGGGNTESGGNIIINGGAITAIGQESSRNTGYGAGIGGGGNGSGGDITINGGTIT
                     AKGAYHGAGIGGGYYTTTKNALSPSVVDPSTGSGYSGNITINGGLSYSYGGAHGSAFG
                     DGCVNPGTNRGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLG
                     GAVAYGDLEKKTKVFMNKISLTYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDAQG
                     ILYLWFSDKFTGSEVSVNLVVIDKITGEVLKPDDFYIKDIGSGSQFLKQYEEFTIDDT
                     PPYLIIKRYDGLPFEEDVEKSFLDWLLKDGGIPITTPKGEKLTDKKFMTISSQRLNEE
                     TMEPDINLGSDDLTKQVDAGKYQLSITSTQFSSIYPFKDAYWGHRAYYKAEIIPADTE
                     TSLVVEESKAKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYKDPVE
                     LKTHNKGSESHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLK
                     LDMTNVDVDGDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGN
                     GDGIWKPDKNIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPN
                     LNKPDPDNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDPDN
                     GGNSKPDPDNGGNGKPDPDNGGNSKPDPDNGGNSKPDPDNGGNSKPDIDTDGDGKPNI
                     NIDTDGDGKPDINIDTDGDGKPDINIDTDGDGKPDINIDIDGDGKPDINIDTDGNGKP
                     DINIDTDESLDSNVGQNVQTGDQSNIMLDLALMFISLFFLIKNLTNKYLRRK"
BASE COUNT         2057 a         1302 c          741 g         2680 t
ORIGIN      
        1 gtttatttca tctatcatct caatcatatc atttactaac tattattcta atgagcatat
       61 aacattgaaa cttataatta tacaacctaa atgcctccca atgtaccctc aaaacatcat
      121 ttcctgtaac tgaagtataa ttggtatatt ttatcactga aataaaaaaa cactagtcaa
      181 taaagactag tattttcaag tattttatca ttcttttttt aattattgga gacgaatcat
      241 cagaataatg cccataattt atctatatgc aaattctaaa tttaaaataa agtttcagat
      301 aaatttacta ttctatgcta tgatacaaca gaaaaaactg aaatctatga gtggtagctt
      361 catataatcc tagttcctac agtaatcaaa actgaggttt attctatatg taactaatga
      421 taaattttat tataggagtt attagatcta tttgtgttac tataagtagt aattatatat
      481 atgataattt tttaacactt ttctgttggc tatatagaaa gtggtttaga atttaaattt
      541 aaataaaatt taaattcacc aaaaagaaat ctatattaga aaccactact ctattccacc
      601 agattgtaat tttttgttct ttatatcaca atgctatacc atattaaatt gaaaaataag
      661 ttttaaattt atttgttaca attcattaat tcaaaaaaat attttgagtc atgagttttg
      721 taaagattta gccatgaatt taatgcttct gattcaaaaa catctaaaag tttcaatact
      781 tgaaacgtaa attccaacgg agcaattcct gaagccgtaa ctaaattttc atcactaact
      841 acagattccg ttttataata tttttctcca gagtaatttg gtattaccat tttaagaaac
      901 tctaagtcat tacttgtatg atttctaaaa tctaatagcc cttttttagc taggcctaat
      961 gtagctccac atatggctgc aactatagaa tcttgtctta aagcttcttt aactttttct
     1021 aatattatct catgattcat agataaccaa gtatttcccc ctggcaaaat taataaatcc
     1081 ttactttcca tattaaactc atcaactgta atttcaggta aaacttttaa cccacccatt
     1141 gttataatag gatttttatc aattcctaca gtaattactt ttagtggctt taatcctttt
     1201 ttaaaatatc ttcctgtatt taattcagca gttaaatatg ccacttccca atctgacata
     1261 gtatcaaata catacatata tacttttcta tttttcatct tttttactcc ttatgtaatt
     1321 aatttatata tctaattata aaataactta actgacatat tcagtcagtt aagctattaa
     1381 atttgataat aatcaattaa ttctagtaaa tcttcaatta ttttcttcct aaggcttagt
     1441 ggttcaatta cttgtattgt ctttctataa ggtaaaagta tatttggtat gtacttgcat
     1501 attacttcct cttcaagcat aaatattatt ttattaggtg ttctttcttt caagtagtgc
     1561 cctaaaaacc aatgttggct taaactatct aaagaatcta tactcccttc tataactaat
     1621 tgaattatat tttgttcatc ttttatttca ggtataattc cttttataaa aaattcactt
     1681 gctgaaaatt ctgtcggtct gttaaatgta tttgatgttt ctatgatttc actaatccta
     1741 tccactctga aactacgaac ctcatttctc atattacaaa atgcaactac gtaccaatta
     1801 ttattccagt gaacaattcc atatggattt ataattctat attttgattt tactttacga
     1861 cctgcatgat agttgatttc tacagatagc tcctttaata tactattctc caatttttta
     1921 agtactgatt ctatagacac tttcccaatt ggttttatta cttcaagggc tcttaagtgg
     1981 tttgttaaaa tatcttcttg tttttgattt gaatacattt ttaactttaa tatcgattta
     2041 tctaatgctt cgtctaagaa gtatccttct tttttagcaa atagtgctgc atgaagtaga
     2101 gatttttgct cttctatatc aaaaaaaaga ggtgctttag taaaattatt taaaaggcta
     2161 tacccaccat tatgacctgt atctgaaatt ataggtactc cactcgcaga aagtacatca
     2221 atatatcggt acactgttct tatatttatt tctaattttt cagctatctg ttttgctgta
     2281 atttttttgt cagagtttaa catccacagt atagacatca tattgtcaat tttagccatg
     2341 tagtttcacc tctttctatt tgttatataa agttattata taactttttg attgattttt
     2401 tacaaaaaaa cattagtaaa ctgctaataa tttatactaa gatttatttc aaatatttat
     2461 agatagtctt tttctatagt taagttatcc attaaattac atagtagtat atctatttta
     2521 gttgatatat tttcagctac ctcccttgaa tattattaca ataagtctaa atcttctttc
     2581 ttccataaat atattatgag gaaagactat catctttaat ggtttaatag tataaaagat
     2641 attaatttaa aaaaatctat ttttatatca gatgggtatt gttttttata aaactgaaaa
     2701 aatcacctaa tagaatctat gattttttaa cataacctcc ataaattagt tccaattatc
     2761 acttcaaatt caaattttta tctgaagaat atacaatcat atttccatac tcaacataat
     2821 attgagtgtt tggcttgtat ttacaaatta ttattctaac aaaaacaact atacatagat
     2881 taaaatcatc ttctaaacat aactactaaa attgaattaa tgatatcaat tccattaatc
     2941 tcttttaata aaaaattctc ttttctatat gaaaattatt tcaaaacttt taagttaaat
     3001 acaaaaatga ctataaatag atttttattt acacaaatct atttatagtc actatagttt
     3061 atccaataaa ataataaaat aaatatgtac acaataaatg ttatttacgt cttaagtatt
     3121 tatttgttag atttttaatt aaaaaaaaca agctaatgaa cattaatgct aaatctagca
     3181 ttatattgct ttgatctcct gtttgtacat tttgacctac attactatct aaactttcat
     3241 ctgtatctat atttatatct ggttttccat tcccatctgt atctatattt atatccggtt
     3301 ttccatctcc atctatatct atatttatat ctggttttcc atctccatct gtatctatat
     3361 ttatatctgg ttttccatct ccatctgtgt ctatatttat atctggtttt ccatctccat
     3421 ctgtgtctat atttatattc ggttttccat ccccatctgt atctatatct ggtttgctat
     3481 ttcctccatt atctgggtct ggtttactat ttccaccgtt atctggatcc ggtttgctat
     3541 ttcctccatt atctggatct ggtttaccat tgccaccgtt atctggatct ggtttgctat
     3601 ttcctccgtt atctggatcc ggtttgctat ttcctccatt acctgaatcc ggtttgctat
     3661 ttcctccatt acctgaatcc ggtttgctat ttcctccatt acctgaatct ggtttgctat
     3721 ttcctccatt acctgaatct ggtttgctat ttcctccatt atctggatct ggcttattta
     3781 gatttggatt acctgtatca taacctcctg agccagtatc tacattttta tcaggcttcc
     3841 aataatcatc tactccatca ttatctgtat ctattgctgg acgattataa tctgtatcag
     3901 ggtttccatc tccattggta tctatatttt tatcaggttt ccaaatacca tctccatttc
     3961 caccttctgt actaggcttc cattcacctg tattatcagt atctatgtta atatctggct
     4021 tccaatcacc actcgtatct atattgatat ctggccttcc atcaccatca gtgtctatat
     4081 ttatatccgg tattccatcc ccatctacat ctacatttgt catatctaac tttaacttct
     4141 tttcatcctc actaccttca atgtaattta tatcatctcc aatatatttt acagtaatag
     4201 tttgttcagt agtaaaaata tgatgattgc tatctaaagg tctccattca atagatgcag
     4261 tactataatt atgactctca ctacccttat tatgagtttt taattctact ggatctttat
     4321 attcctttcc attaatatag aattgtacgt aacctcttgg agaggcacaa tctggtcctt
     4381 ctttcttatc tggtgaaata gtaacattta atgtaaacgt atctgttggt ttaaaaacat
     4441 cagttttagc tttagactct tctacaacta atgaagtttc tgtatcagca ggaataattt
     4501 ctgccttata atatgctcta tgcccccaat aagcatcctt aaaaggatat atactcgaaa
     4561 actgtgttga tgtaatgctt aattgatatt tcccagcatc aacttgtttt gtaagatcat
     4621 cacttcctaa atttatatct ggttccatcg tttcctcatt taaacgttga gaactaatag
     4681 tcataaattt tttatctgtg agtttttcac ctttaggagt agttattgga ataccaccat
     4741 cttttaatag ccaatctaaa aatgattttt ctacgtcttc ttcaaatggt aatccatcat
     4801 atcttttaat aatcaaatat ggtggagtat catcaatagt aaattcttca tattgcttta
     4861 aaaattgaga acctgaacct atatctttaa tataaaaatc atctggtttt agaacttctc
     4921 cagtaatttt atctataaca actaaattaa cagatacttc agatccagta aatttatcag
     4981 agaaccataa gtaaagaatc ccttgagcat ctgtataact aggtgctcca taaggataaa
     5041 tttttccatc aattagcatt ttaaaatcat caactagtgt agtactaact ttatcttgcc
     5101 ctaaatacgt tagagatatt ttattcataa aaacttttgt tttcttttct aaatcaccgt
     5161 atgcaactgc tccacctaaa cttttgaatg ttgttgcttt taatgaacca cctgttacta
     5221 taacatctcc cgttgtagca cttaggtcaa aaatccaatc tcccactcct gtcatataag
     5281 gtaaaatcgt tcctccagtt acaagtatac gatatcctct atttgttcct ggattaacac
     5341 atccatcccc aaatgcacta ccatgtgcac ctccatatga ataggaaagt ccaccattaa
     5401 ttgtaatatt cccactataa ccagaacctg tgctaggatc tactactgag ggacttaatg
     5461 cattctttgt tgttgtatag tatcctccac ctattccagc tccatggtat gcacctttag
     5521 cagttatagt accaccattg attgtgatat ctccaccact accattaccg cctccaccta
     5581 ttccagctcc atatcctgta ttacgactcg attcttgacc tatagcagtt atagcaccac
     5641 cattaataat aatatttcct ccactttcag tatttccacc gccaatacct gcagaataat
     5701 aatttccaac aacttttaaa cttcctctat tattactatc tagtagtgta aggcgatctc
     5761 ctttttttaa cttgtctcca ttttctagag ttacatctct aggaattttt ccaccctcag
     5821 aaactataaa ctcgccttca atatctctat ttacaacaga atcatcaata attagagtag
     5881 taccttttcc tgctctcata cctggataat atacatcacc tgactctagc ttgttttcac
     5941 taccatcttg taatataaca tgtgcatatg caccaccatc tttatctggt gttaaagtaa
     6001 atgggctatt agatgcagtt ttaatattta ctccatcaaa tattaattca gcttttacat
     6061 ctttaggtat aacaatccta tgtgttgttg gagtatttgg attattattt tgaattgtca
     6121 tttgttttcc actaagaata gttaaaacgc cacttgcaaa tttaaaatcg acatttattt
     6181 ctccaccact aattttaaag tctcctacag atgtaggatc ttctgcagtg gcttcttcca
     6241 caactaatgc attcatatct ccttctgcat ttcctgcatt agaatcttct tctgcatttc
     6301 ctgcattaga atcttcttct gcatttcctg cattagcatc tccttctgca tttcctgcat
     6361 tagcatcttc ttctgcattt cctgcattag catcttcttc tgcatttcct gcattagcat
     6421 ctcctcctgc atttcctgca ttagcatctc cttctggatt tcctacatta gtatctcctc
     6481 ctgcatttcc tgcattagca tccccttctg catttcctgc attagcatcc cctcctgcat
     6541 ttcctgcatt agtatctcct tctgcatttc ctgcattagt actttcttct atagtatctg
     6601 cgaaagtatt tgacgcatta aatgatgcta ctgatccaag tcccattgag atacttaaaa
     6661 tcattgatag tgctttcttt ttacattttt gttgcataaa ttccccctcc ccttaataat
     6721 ttttaatata cactgtatac atccttatat acactctaat aattcttaat atacactgta
//
LOCUS       DAEOSP010000186         6567 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.190, whole genome shotgun sequence.
ACCESSION   DAEOSP010000186 DAEOSP010000000
VERSION     DAEOSP010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.190"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(259..2646)
                     /gene="cadA"
                     /locus_tag="KRH54_003006"
     CDS             complement(259..2646)
                     /gene="cadA"
                     /locus_tag="KRH54_003006"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBG8580641.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDIVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2669..3034)
                     /locus_tag="KRH54_003007"
     CDS             complement(2669..3034)
                     /locus_tag="KRH54_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8580642.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3233..4081
                     /locus_tag="KRH54_003008"
     CDS             3233..4081
                     /locus_tag="KRH54_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580643.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSVQGIPQATNIEE
                     FDEEEM"
     gene            complement(4518..5606)
                     /locus_tag="KRH54_003009"
     CDS             complement(4518..5606)
                     /locus_tag="KRH54_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580644.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5731..6459
                     /locus_tag="KRH54_003010"
     CDS             5731..6459
                     /locus_tag="KRH54_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG8580645.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
BASE COUNT         2305 a         1099 c          715 g         2448 t
ORIGIN      
        1 atgaatctaa tttaaactta atataaactt attaataaac cttctatgtc taaattttaa
       61 aattacaata taaaagctct acactaaata aatacattaa ataaacataa tggataatga
      121 atataaattt atatatgcaa agcgctccaa taaaaaggta ccaataaagt taaaagaaac
      181 ttattgatac ctttcattat tacatacttc aatatataat tataagtttt ttaaatagta
      241 tggatttaca tttatccctt atctcatagc tctcatagca tttactactg ctataagtgc
      301 cacacctaca tcagcaaata tagcttccca catagtagct actcctccag cacctagtat
      361 cataactata atttttactc ctagagcaaa tattatgttt tgccacacta tcttattagt
      421 cttgtttgct atttcaattg ctttagatat cttgctaggc tcatctgtca taagcactac
      481 atctgcagct tcaatagcag catctgaacc taagcctccc atagctatac caacatcagc
      541 acgagctaat actggagcat catttattcc atcacctaca aacgctattt tctctttttc
      601 agttcttcct tcatataaat cttctaatct atctaccttt tcattaggaa gtaaatttga
      661 gtaaactgta tccaattcta attcttgagc aatatttttt gctactttct cattatctcc
      721 agttaacatt acaacttctt ttacccctat ttcttttaag cttcttatgg catttttact
      781 atcttctttt acttcatcag agataactat ataacctcta tatactttat caacagcaat
      841 atagactact gtgcctactt ctttagctga tgaataagat atattttctt ttttcataag
      901 tttctcatta cctgctaata taaaattgcc attatgcttt actctaattc cataagctgc
      961 tatttcctca taactgtcta ttgtatctaa atcaattttt ttattatagt aacttaatat
     1021 ggactttgct attggatgat ttgaatttac ctccgcaata gctgcatatt ctaataattc
     1081 ttcttctgat ataccttcag gatttaactt agttacattg aacacacctt tagtaagtgt
     1141 tcctgtttta tcaaatacta ctatatctac acttcttaaa gcttctaaat agttacttcc
     1201 tttaataagg ataccatttt tagatgcaaa cccaattcca ctaaaaaaac taagtggtat
     1261 tgataaaact aatgcacaag ggcaagaaac gactaagaat atcaatcctc tatataacca
     1321 atcactaaat acctcacctg agattacaag tggtggcaca aatgctatta ataaagcagc
     1381 tattactact attggagtat agtatcttga aaacttagat ataaagtttt ctgtctttga
     1441 ttttttaatg ctagaatttt caacaaggtc aagaatctta gaaactgttg attctccaaa
     1501 actctttgta acttcaatac tcaataaagc atttttattt ataacacctg ataagatttc
     1561 atctcctttt tcaacttctc tcagcactga ctctcctgtg agtgcagaag tatctaacat
     1621 agaaactcca tctactacta caccatctaa tggtactttt tcaccaggtt ttactactat
     1681 tatatcacct acatttacct cttctggtga caccacctta acttcagaat taacttttag
     1741 atttgcatag tctggtctta tttgcataag tgaagttata gactttctgg atttaccaac
     1801 tgcaacacct tgtaaatact ctcctacttt atagaaaagc ataaccccta ctgcttctga
     1861 taactcccct attgttaatg ctcctactgt tgctagagcc atcaaaaagt tctcatcaaa
     1921 tactcttcct tttgatatat ttcttattgc tcttaaaagc acatcaccac ctactattac
     1981 ataggcaact ataaatacta tattgctaaa ctgactttca taacctgtag ctgtttgata
     2041 tataccaaat atatatacaa gtgctccaat tataagtgga attaattctt tcttatttgt
     2101 atcatttaca gctcctgatt tttcagtagt tctccctttt actttgtcag tagaccctac
     2161 ttgtatatca agccctggct ctgttgaatt tattatttct tttattttat ttataacatc
     2221 atcctcttca aagttattac ttatatttac tgtaagtttt ttatttacaa agtttaaatt
     2281 agaagattca acttctttta atttagaaac tttgttatta atctcttctg cacaatgagc
     2341 acagtttaat cctcctaata taagctcttt tttaattgat gttttactag ctgcattttc
     2401 tttagaactt atttgtatat caagacctgg ctctgttgaa tctattatat ctattatctt
     2461 ttctatagta gtatcctcat taaaactttc ctttatattt actgtaagtt ttttatttat
     2521 aaaatttaaa ttggaagatt taacttcttg tagtttagaa actttattat taatctcctc
     2581 tgcacagtga gcacaattta atcctcctag tataaattct tttttaattg aattatttgc
     2641 ttccatatat attcactcct ctcatttatc atttataagt ctctctaata tgatttaatc
     2701 cacaatcaaa tatctgactt atatgactat catctaatga gtaatacact gtttttcctt
     2761 ctcttctaaa ctttactaat ctagcctgct ttaatactct aagttgatga gatattgcag
     2821 aatgagtcat atttaataaa ctcgctatat cacatacaca catttcattt gcaaataatg
     2881 catataatat ctttactcta gtagtatccc caaatacttt aaataattct gctaaatcat
     2941 ataacatttc ttcatctggc atagttgatt tagcctctgt tactatttct tcatgtacta
     3001 tattacaatt acaatcattt atctcttctc tcatttttaa tcctccatta ttttaatata
     3061 tgaataactg ttcatatgtt cattatatgt tatcatttct aaatttgcaa caataatttt
     3121 tattaattta atataaatag aatgcacata ttataaactt ttttaatata tatattatta
     3181 gaataagttt ttgtaaactt ttttatagtt ttctaggagg tgcaagttta atatgagtaa
     3241 caagaaaaaa aaggatttag atactagtta catgccttac ttagatccag cgaactatgc
     3301 agattatatt actaatgaag aaattcccca agatgaattg agaaatgacc ctcaaatttc
     3361 taatggttat acaaaaatac ctaataacca gaacataaat aacctatatc ctaataatgt
     3421 aaatcattat cctaaaagta gtaattctcc aaacaacttg cctaacacaa ataatacatc
     3481 cggaaatatg aattacaata catctgctac aaataatatg cctaataaca tgaataataa
     3541 tacgggtatg cctaataata tgtctaacaa tatgaatagt aatatgggta tgcctaacaa
     3601 tatgcctaat aacatgaata gtaatatggg tatgcctaac aatatgccta ataatatgaa
     3661 tagtaacatg ggcatgccta acaatgtgcc taataacatg aatagtaata tgggtatgcc
     3721 taacaatatg tctaataata tgaatagtaa tatgggcatg cctaacaata tgtctggcaa
     3781 tatgaataac aatatgtatg gtaatacagg tatgcctaat aacatgaaca gtaatatggg
     3841 gtataatatg aacaatacta tgggtattcc taacttctca tgtaatcaaa atatgcctcc
     3901 taatgtactt atgatgatac caggagttat atgtcataat acaatgcaag gtatgcctgt
     3961 agttatgcct agtactatgc ctcctaatat atatcctact ccttatggtt ctagtaatat
     4021 gtctgtacaa ggtataccac aagctactaa tattgaggaa tttgatgagg aagaaatgta
     4081 atatatattt ttttaagtaa gttgtataaa acattaataa ttgaccataa taataaatgt
     4141 attcctcata atattccccc aatattatta taggtataga aagttgactg aatatgcgct
     4201 tattcggtca acagatatta acataatcta ataagataaa catagaaaaa gtcagataaa
     4261 atttaatttt atctgacttt ttcatttaaa aatgctctct tgtgttggtg attcagaata
     4321 gtatggtact cgtatttgag actaatattg gcaaacaaca taaagaaaaa tataaatttt
     4381 ttataataaa ctgtataaaa ttttaataat tgaccacaat aataaatgta ttcctcataa
     4441 tattccccca atattataga aataaattta agcgagatta aattcagtta atctcgctta
     4501 aataatgtct aaatttatta ttccaaaaaa tcatcaaaat ctaatgaacc tgattctgca
     4561 aaatcatata atgcattttg ctcaaagaaa aatttctctt caaatttaat atagtcgcta
     4621 taattagatt gacttatttc attgatataa ttgcaatcat tttcgttata agtaccaata
     4681 aaaaacttat ttcccattaa gtaatatata gctttcatat cattatttat tatatataca
     4741 ttttctttta catggtcatt attaaaatta aatctagtaa agaatatacc ttcattcata
     4801 ttacttttta acagaaatgg attttctcta taaaatactc ctgcaaattc ttcaggtgaa
     4861 tttacactgt aaacacttac aaattcactt ttagaaatct catcaaatac ctcaaaatca
     4921 tacataggtt ctttatctgt cactaataga gaattaattc taaaaccagt ttcataacac
     4981 ttacttatgc aaaaagcaat tttacatata taatatccat cattatcttc atataaagct
     5041 tcacttatat atctgccttg acctttatca cttaccacgt ttttaagtaa tgttccattt
     5101 atattagtaa tgtgcatatt tgcaatctcg tcacttccag aaaagtactt taaactttct
     5161 ctatcccatg ctataaatct catggtcata tagtttataa attcaacttc ctcgtcaact
     5221 cttttacata ttttattgta taatttttct atagttaaat ctgttttata gttttttata
     5281 atatcaatat actcacattt atctcctggc aatagcttat cataataaag attcttatta
     5341 ccaaaataag atactaaaaa taaagattca tccttcgtta attcaactct atctgaacca
     5401 aatcctccca tcaatctttc ttcttccata taagcttctt cttgtgttgg gttatttaaa
     5461 ctcacataat ccgctaatcc taatccctct gcatctaata aaaaatattg acaaaagtga
     5521 ttgttttcat catctatcca atttattata agacctaaac tgcccataag tctagaattt
     5581 gtagcctttg caaaattaaa attcatatag tcacatcctt taaactctaa aatacttgtt
     5641 ttttaatata taaattatta tacatatatt ttaacaactt agatacaatt tataatataa
     5701 tataaggaaa tgattaattg aggtggtctt atgaaaaata tagcagcttt ttttgatata
     5761 gatggtactc tttatagaga ctctcttatg gttgaacatt ttaaaaaatt aattaaatac
     5821 gatataattg accaaaaaaa ttggtataaa catgctagag acacttttat ggattgggat
     5881 aaaagacaag gtaattatga tgattatcta ttagaaatat gtgaccttta tgttgattca
     5941 ctaaaaggat tagataaaac ctgtatcaac tttacaagtg accaagtaat taaattaaaa
     6001 gcagatagag tttataaata cacacgttca aggattgagt ggcatctaaa tcaaggacac
     6061 atagttatat ttatatctgg tagccctgga tttcttgttg aaaaaatggc aaaaaaatat
     6121 aatgtaaccg attttctagg aagtgattat gtctttgaaa acaatatatt tacaggcaca
     6181 gttattccaa tgtgggattc tataagtaag aataatgcta taaacgattt tgttgtaaag
     6241 tatgatttag atttatcaaa ctcgtatgcc tatggagata ctaatggtga tataaatatg
     6301 ctaaaaagag ttggaaatcc aatagcaata aatccaacaa aggaactttt atctcaaata
     6361 tcacttgatg aggaaatttc taaaaatgtt gagataatag tagaaagaaa agatattatt
     6421 tattctctat ctacagatgt aaatatagtg gatatttaat ttatactatc tattttaaaa
     6481 acttatatta tatctaatat ttatatctaa catataaaag ctttgactaa aaatcaaagc
     6541 ttttatatgt tgcactaaga atatatt
//
LOCUS       DAEOSP010000187         6536 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.191, whole genome shotgun sequence.
ACCESSION   DAEOSP010000187 DAEOSP010000000
VERSION     DAEOSP010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.191"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            110..739
                     /locus_tag="KRH54_003011"
     CDS             110..739
                     /locus_tag="KRH54_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580646.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTKNTMLDCRCPESISLLKRNNLINDFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            740..1522
                     /locus_tag="KRH54_003012"
     CDS             740..1522
                     /locus_tag="KRH54_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8580647.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1515..2393
                     /locus_tag="KRH54_003013"
     CDS             1515..2393
                     /locus_tag="KRH54_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580648.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            2426..3385
                     /locus_tag="KRH54_003014"
     CDS             2426..3385
                     /locus_tag="KRH54_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG8580649.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3699..4391
                     /locus_tag="KRH54_003015"
     CDS             3699..4391
                     /locus_tag="KRH54_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8580650.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4401..5405
                     /locus_tag="KRH54_003016"
     CDS             4401..5405
                     /locus_tag="KRH54_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8580651.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV
                     "
     gene            5653..6504
                     /locus_tag="KRH54_003017"
     CDS             5653..6504
                     /locus_tag="KRH54_003017"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBG8580652.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
BASE COUNT         2558 a          589 c         1202 g         2187 t
ORIGIN      
        1 tagataaaaa ttgttttatt aagaaaaaat taatatgttg acaaaattta agtgctcatt
       61 taaactatta attgataata atattcaata ttaaataata ggaggcgtta tgaataaata
      121 tcttctaata aatccagtgg cagaaagaat gtatggagaa aagtttcacc ttattaaagc
      181 aaatttgata aaaaaggggt acataatagc agaatgtgaa ccacagttag aatatgtaaa
      241 aaagcagtac aaagagtata caaaaaagac taaaaataca atgttggatt gtagatgtcc
      301 agaaagtata agtcttttaa aaagaaataa tttgataaat gattttgaag tacctatgat
      361 agaaccaata ttagtaagga cttgtagggt tttatacgat aaatatataa aaagtaaaga
      421 tgatatgttg gtagtaacct gtccatgtac acaattaaga gactttgcaa gtgaaaagtt
      481 taaggataag ggtaatgcta tgttttatac atggaaagag tttgctgaac aagaaggtgt
      541 aaaatcactt ggaaaaatag atgaatcacc aattccatta ggatatttca aagatacttt
      601 tgaaaaagtt cttgaagtat caagtgaaga cgaaatatta agtgaaatac aaaaaataag
      661 aaatggtttt gaagataagt atgacatagt agaaatgttt tattgtaaag atggttgtaa
      721 taatggagat ggtttgtaaa tggtcaataa atttaataat aagtttttaa ggctagtaaa
      781 gagtgttctg atatttttta taattctttt attatggaaa attacaaatt atcttggtat
      841 ttggagtgat tatatattac catcacccga aaaggtatat agtacatttt taaatatgat
      901 aagtgatggt tcaatattta ttaatgtata tgcaagtatg aaaagagttt tgataggttt
      961 tgcaataagt actgctatag gtgtgccttt agggatattc tttggaattt atagtggagt
     1021 atacgaatac tttaaatctc taataaattt tttaaggaat acacctcccc ttgctttaat
     1081 accaatgctt atattatggt ttggtatagg agaggaatca aaaattataa ttatagtttt
     1141 agcatccttt ttcccaatct ttacaagcac attgaaaggg attaaaaatt gtgactcaaa
     1201 actgattgaa gtgggtagag tatttgagtt ttcaaagctt cagataatat ttaaaataat
     1261 aataccaaat gccattcttg atattgctgt tgggttaaag ttagctcttg gatatagctt
     1321 tagagcaatt attggggcag aacttgtagc agcttcatct ggtcttggat acttgatatc
     1381 tgatggaaaa gaaatgtcta gaacagatgt tgttattgta ggtataatcg taattggact
     1441 tttaggaata attacagatt acatattttc gattattgtg aagaaggtaa gtaagggaaa
     1501 gatggtggaa gcatatgaat aatttagtat gtgactctgg atatataatt agaaatttat
     1561 ataaaagtta taaagttgac aatgaagaac atgtagttct tagtgatatc tctttaaaca
     1621 tagatagaaa ccatataaca gtaattttag gtgaaagtgg ttgtggaaag actaccttgc
     1681 ttagagcaat tgcatcactt gaaggtataa atagtggtga aattaggttt attaatgata
     1741 ataaagagta tagacctaat gttggttttg tttttcagga gagcagatta atgccttggt
     1801 taaacgtaag tgaaaatata gcttttcata atcaaagaaa aaatactatt ttaaacaagg
     1861 tgcttagaaa atttaaacac aataaaagaa ttagcagtaa aattataaat gataatttta
     1921 gaagcttaga aacaaatgat aatttgaaca aatcaaataa atataatagg aaaagtaata
     1981 ttgatgtaga aaaatatctt aagatgatga acttgcagaa atttaaaaac tcttatccaa
     2041 atgaattgtc aggaggtatg gcacagagag taagcatagc tagagctctt tctttcaatc
     2101 cagatatgtt gcttatggac gagccttttt cggcattaga ttattttaca aggattgata
     2161 tgcaaaatga agttataaga atacataagt caactaataa aggcgtgata tttgtaactc
     2221 atgatattga tgaagcgtta aagataggca agaaaatagt tgttttcaca gacgaaagaa
     2281 aaataaaaga atttaatata gaagatggtt ataatagaga tttaacttca gattactata
     2341 ttaaattaaa aaaagatatt ttaatgacaa tgaaatcaaa taaaggcatt taaagaaatt
     2401 aataatattt taaggagtaa agtaaatgaa attaaaaaag ctattgatag gtataacgat
     2461 attaacactt gcaacagcat ctttagctgg atgtgctaaa aaaaataatg gagaaaaact
     2521 ttcagaaata aatttaactt atgtaaaatc accattaaat gttccttcta taattcagaa
     2581 acaggatgat ttatttggaa aggaatttaa aaaggataat ataaaagtta acttccacga
     2641 aattactaca ggtccagagc aaacacaagc tttagctgct ggagaaatag acttcttaca
     2701 tgcactaggt ggtacatcag ctttaatagc tgcatctaat ggagtagagt taaagatatt
     2761 aaacacttat agcaggtcac caaaaggatt tatgattttg actaataata attcaataaa
     2821 atcagcagca gatttagtag gtaagaaggt ggcaggccca aaagggacta tattacatca
     2881 ggtattgatt tcagcacttg ataaagaagg attaagtatg gatgatgtgg aatttgttaa
     2941 tatgggtatt ccagaggcat cagcagctct ttctgatggc agcgtagatg ctgcattgat
     3001 tgctggtcca gcagctctta aagctatgaa gtcaggtagt aaacttgtag caaatggtga
     3061 aggtttagta gatggtataa tagtaactgc tgttagcaca gactttgcag agaaacatcc
     3121 agaaatagtt gaaagattta tgaaggttga aaaggaaact ttagaatatg taaataataa
     3181 ttttgatgaa gctatggaaa aagtagctaa agaagttgat ttaagtcttg aagaaactaa
     3241 agaattatat gcttggtatg attttagttt agatataaca gataaagaca tatcttcgct
     3301 tgaagataca caagacttct taataaaaaa taagttacaa gagaaaaaag tgaacataaa
     3361 agaattaatc tataatgcta agtagaaact atgataaaat aatactaagt aagaaatata
     3421 ataaaagttt aagttacctt attaaggtaa ctttttctat tttgaattag taatttcaat
     3481 ttattttaga atgattagtt gataataatg attaattaat cataaaaaga tactttttta
     3541 tagagtttta ttaagatgtc taataatatg gttatactta tgaagtcagt aattgtgaag
     3601 ttagtaatat gattaaattt atatattgca aaaagattat aatatattaa aattggactg
     3661 ttatatgatg agtaaatagg tatattagga ggaattatat gagttataaa gtatatattg
     3721 tggaagatga tatatcaata agtacattac ttaaagatta tattgagaaa tatggatttg
     3781 aagtgattgt tgaagagaac tttgatgata taatgattac atttgaaagt tttaagccac
     3841 acttagttct attagatgta aatttaccta aatttgatgg tttctactgg tgtagatgta
     3901 ttaggcaaca gtcgaattta cctataattt ttatatcagc tagagatggt aatgtagacc
     3961 aaattagagc tttagaaagt ggagcagatg attatataac aaaacctttt tactatgatg
     4021 tagtaatggc aaaaataaag agtcatataa gaagggtata tggtgaatat tcacctaaaa
     4081 tagacgaaag aatagtggag ctagatggat taaagtttta tccagaaagg ttagaactag
     4141 aattaaatgg tgagaatcta attataacta aaaaagaagg tatactgatt gaatgtctta
     4201 tcaagagata cccaaaagta gtaagtagag attatctttt agaaaagata tgggatgata
     4261 ttgagtttgt agaggaaaat actttaaatg taaatgttag caggataagg aaaagatttg
     4321 aggaattagg tatagataat gctgttcaga ctgtccgagg gttaggatat aaattaaata
     4381 agaattggta aggagaaagt atgaagttat ttttaaaaga ttataaaggt tatattttta
     4441 tatattatat aggcatagtt atgacaatta tttactgtaa tcttatgaag tttattagtt
     4501 ttggagagag tttatatatt cttttattta gttcttttat attgttatgt tttttaatat
     4561 ataagtatta taaaaataga gaagtatata atctgtttga aaaaggagta cattctttgg
     4621 aggagtcatt tttgtattta ggaagctcat tcttggggag aaatatatca gttattttaa
     4681 aacaacaaca taaattgtat caatcaatga tacaagagca taaaaagata catagtgaac
     4741 atttaacttt catcaaccaa tggattcatc agatgaaaac accaatatca gttataagcc
     4801 ttcagcttca agattatgag ggagaagaag taactgaaaa tataagaaaa gaaatagata
     4861 gattaaataa agggttagat atggctatgc attttgcaag ggcagataat tttcagaaag
     4921 actttatagt tgaaaaagcc aaattaaatg aaattgtatc tagtattgta aatgaagaaa
     4981 aaaggttatt tataaaaagt ggaatgattc ctaagattca agtagacaaa tttacttatg
     5041 tttatacaga tgtaaaatgg atgaaattta taataggtca attaattaca aatggagtga
     5101 aatattctaa agattatagt aactacctaa ctataaaatc tgaagaaaat aataaatatg
     5161 taattttaag tataatagat gaaggaattg gaattgtttc taaagattta aaaagggtat
     5221 ttgacccatt ttttacagga gaaaatggtc gtaagtttgg agaatctaca ggtatgggtc
     5281 tttatctagt aaaaaaggta tgtgatgatt taggtcataa agtaagtata aaatcagaag
     5341 taggaaaagg tacagaagtt tcaatctact ttaataaaga tgtaaatagt ttaataaagg
     5401 tgtaaattat ctgttacaat agggttttta gttaattgta cttttttatt atacaattta
     5461 ttaaatgaaa tattcatagt taaataatca aaatttaaga gagtttattt gcaaacctta
     5521 catagatgta aggtttgtgt aatgtttgca gatagttaaa ttttctaatc taaattacaa
     5581 ttaatataag aaataacata agagaaataa cataaaaaaa gaaattttgt aaaaaaggaa
     5641 ggatgtttat tgatgagttt agatgttatt tttatattga aatcagtcat aatagctata
     5701 gtagaggggc ttacagaatt tattccagtt tcatctacag gtcatatgat tttggttggt
     5761 aatttaatag attttaaggg gcaatttgct gaaatgtttg aggttgtaat acaacttggg
     5821 gctatattag cagtagtagt actttattgg aaaaagataa aagatagcgt tatagaattt
     5881 tttaaattta tatttactgg cggaaaagaa ggaaagattg gttttagatt tggaatgaat
     5941 gtaattatag gttgtatacc atttgcgatt ataggagttc tattttacga taatataaaa
     6001 tcattgttta atttacaatc tgtaattata ggatttatag tgggtgggat tttattatta
     6061 gtagtagaaa ctttgtttag aaaaaagaat cattccacag ataatataga taaaattact
     6121 ccaatacaag ctttaaaggt tgggacatta caagttttgt ctgcatggcc aggtatgtca
     6181 aggagtgcat ctactattat gggtggatgg atagcaggac ttaattctcc tacagcagct
     6241 gagttttctt tctttttagc agtaccagct atggtggctt cttcaggaaa agatttattt
     6301 gaatttgatt attcaataat gacaccaaca ttgtggattg ctttggtagt tgggtttata
     6361 gtagctttta ttgtatcaat aatagttatg gaaaaattcg tgaatttttt aaagaaaaaa
     6421 ccaatgagag tgtttgctgt atacagaata atcatgggtg tagtacttgc agtattagct
     6481 tttactaaca taattagtgt atagaaaaaa tagatttatt atctataaaa attaaa
//
LOCUS       DAEOSP010000188         6506 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.192, whole genome shotgun sequence.
ACCESSION   DAEOSP010000188 DAEOSP010000000
VERSION     DAEOSP010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6506
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.192"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            30..230
                     /locus_tag="KRH54_003018"
     CDS             30..230
                     /locus_tag="KRH54_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580653.1"
                     /translation="MEKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            complement(437..1027)
                     /locus_tag="KRH54_003019"
     CDS             complement(437..1027)
                     /locus_tag="KRH54_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBG8580654.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(1030..1905)
                     /gene="dpsA"
                     /locus_tag="KRH54_003020"
     CDS             complement(1030..1905)
                     /gene="dpsA"
                     /locus_tag="KRH54_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBG8580655.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            complement(1981..3372)
                     /locus_tag="KRH54_003021"
     CDS             complement(1981..3372)
                     /locus_tag="KRH54_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBG8580656.1"
                     /translation="MNYYELLRIKKERQKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKKLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIINIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            complement(3376..4512)
                     /locus_tag="KRH54_003022"
     CDS             complement(3376..4512)
                     /locus_tag="KRH54_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBG8580657.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYDTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(4544..5089)
                     /locus_tag="KRH54_003023"
     CDS             complement(4544..5089)
                     /locus_tag="KRH54_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBG8580658.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            5413..6192
                     /locus_tag="KRH54_003024"
     CDS             5413..6192
                     /locus_tag="KRH54_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580659.1"
                     /translation="MKVNENNINNIDEELGELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEISLSQREHFKI"
BASE COUNT         2266 a          998 c          776 g         2466 t
ORIGIN      
        1 atttgaagtt tataagaagg aggataagca tggaaaaatt aaaaacaatt agcgtttttt
       61 ctttgattat aagtgtaatt ttaacgattg gaggtattgg aatagtaact tattatgtag
      121 ataatttgtt tattagagga ctatcagtat ttgttttaat tatgtcctct tcttttgtat
      181 caacaacagt tagattgata tttgaagagt caaaacgata taaattctaa tttaaaaatt
      241 ggattaagta aataatttta atatagcaaa aattacggag attagctcaa aatagacttt
      301 ttagtccatg aggcattttt ttgtatgaaa ttaaatatat tttcatcatt tcaacaagaa
      361 aaagaggctt ctctctattt tgagacagcc tctttgctag tattgtatct aatacataat
      421 attataagaa ccaattctat tgatgtggac tttttataac aggctgtagc tgtttgttct
      481 ctagagcaag ttctatagtt ggaattatta gctcagtatg tgcaatcata gaattaggct
      541 ttgctttgca attatcttga ccaaaaggta caaaatatat atttttagaa ttaaggagaa
      601 tgccaatatt tttgaaatta aagcttagag catcattcgt tgatattgat attactagtg
      661 gtttatcatt acgaagatga ccttttgctg ccattaatac tggtgaatct gtaataccat
      721 ttgctaattt tgcagcagta tttccagtac aaggagctat tattataata tcagcaattc
      781 ctttaggtcc aaatggctct gcctcttcaa tcgtaactat aggtttattt cctgtaagtt
      841 catatgcttt tttcataaac tcttcagaat ttccaaatct acaatcgata ttttgtgata
      901 catctgaaaa tatagtgtgt acattagctc cttctttaac caaattctct aactctataa
      961 atattttatc ataagtacaa aacgaaccag taaatcctac tccaattgta agtccttcaa
     1021 gtctcatttc taatcccttc tttcatttat aatattttct atagcgttaa caagaatttc
     1081 accagatgtc tttggtgaat atttaccagg aagcccaagg caaagagtcg cattaatccc
     1141 tgcttcttta acaacagaat aatctactcc tccaggagaa gaagctatat ctataatagt
     1201 tacttcttgt gatacttttt ctaacctttc tttcgttaaa atacactctg gtatggtgtt
     1261 aaaaatataa tcaaattgta aaagtttttc atcaagttga cttaaagaaa tactggaata
     1321 tccaaatgca ttagccgtac ttcttgcatc attatttctt gcgccaacac atacatttgc
     1381 atccaaagcg tataattttt ttgcaagtat ttgtgcacat cttccataac ctaatactaa
     1441 acagttactt tgatgtatat tgattatact tcttttaata gcttctgcta tagccccttc
     1501 agctgtagca actgcattta aaatagcaac atcattcatt ttcataaaat cataaatctg
     1561 aatagagtgt tgctcgcaaa atttacaaac tttagttggt atgttacctc caaataatat
     1621 ttgattagga gataaattaa ttagcaagtt atcaatagta gcatcaagac aatcatccgt
     1681 attttcaata ttaacttgat tctttgtaaa tggaattgga cataaaataa tggaactttt
     1741 ggatagtgct tcagctagtg aagttgattt tccaacgatt ccatttataa tttcatttga
     1801 tatcccataa gtgactacag aatacccttt attaattagt ttttttacca tgtacacttg
     1861 tcgcaggtct cccccaataa cagttatatc gtattgattt gacatattgt aagcctcctt
     1921 tactatatac aatatgtttt tatcaataca ttggttcact aaaattacta aatattatac
     1981 ttataacatt tttaataatg ctaatctatc tatcttttca gaagaggtat atggaaatga
     2041 atttaaaaag ataattttct ttgggatttc atttttcatt agattatctt tcattttaat
     2101 caacaaatct ttcttcgtaa ttttgtctat agttattaca aataatacaa tttgatttcc
     2161 tcttatagtg tctgagtacg gcaatacaat tgcatcttca atttgaggaa tttttttaac
     2221 ttcattttca atcttagtag aactaacttt aaatcctcca atatttataa tgtcatcact
     2281 tcttccttca aatattaagt atccatcatt atcataatat ccaatatcat tgacagaata
     2341 aggtttggtt gcattatata ctgcatattc tgtattcaca aaaatttttc cttctctaat
     2401 atatatatct attcctggaa atggtctgcc tacacttaga ggtttttcaa ttagttcatc
     2461 atagcataaa tatgtaatat aatttaattc acttgcccca tagtataaaa taatttcaga
     2521 ctttggcata tattttttta gatttcttgc agtatcttca aataataatt gagaccctgt
     2581 aaaaatactt acaactttga gtactggttc ttttaaatgt ttaaccaaaa gttgaagttt
     2641 tgtaggtata aggtatatgt tagttacatt atattgtttt attgtcctta tccatgattt
     2701 acaattaaga cctgaactta ttacaatact acctccttca tataatactg acattataga
     2761 atttagattc cctgtaaaac taagtgttcc attaacaaac aagattgatt ccccagagat
     2821 attaaaaata ccattttgta taggaaaaaa tccagcccaa ctctcatatg ttctatataa
     2881 tacctttgga acacttgtag aacctgaaga taaagctccc atacaaatac ttttatctaa
     2941 ataatttata agcttcttat attgatacat atatatatta aatttaggat taaaaataga
     3001 aaaattaact ttttttgcta cttcattatt gttatcatca tataaatcaa taacgtccat
     3061 agattcatca gatataatga ttgaaatatt attttttaat aaaatatcat ctaatacttt
     3121 tttagaaaga ttataatgac aaataatagg aatatttttt gaataattaa tagcaaaaaa
     3181 actaatcaac tgaaacatta tatttttaga atatattaaa attgggatat tttcacctaa
     3241 agaaatttgc tttcctagtt cctcactata actcaatata ttttcataag tatatttttc
     3301 tccatctata attaaaaaat ttttattttt ttgcctctct ttttttattc ttaaaagttc
     3361 ataatagttc ataatctaca ccctttcaag cagtaaagct gaacctagac caccagctgc
     3421 agctatagag caaattccat atctaccctt agtcttttcc aatgctttta aaagatgtaa
     3481 tgcaataatt gcacctgaag caccatatgg atgtccatac gctaaagctc caccaaatat
     3541 attatatcta tctattaact ctggatactt tctttgaaac aacacatcta taactgcaaa
     3601 tgcttcatta aattcaacag cagatacatc taaataatta agactttcta tctctaaaag
     3661 tttatccatt gcttgtatcg ctgatgttgg acttaaggtt gcatctgttc ctattgtgca
     3721 tgtattaata atttttgctt taggcttctt ttttacatgt tctaaatact tcttagaaca
     3781 tattataata aatgaagctc catcattaat aagacatgaa tttgctgcat ttgtaatagt
     3841 ttcttttcct aaaattgatg gcatcctatc taataacctt tgactcatct tatcacgtat
     3901 accttcatca taggtactat tatttatagg agatataata tcctcaagta ttttttcttc
     3961 tcttgcttct tttgctcttt tatgactttc ctttacccaa aaatccaagt ctgctttctc
     4021 aatttgatat agctctgcta ctctttccgc cccttcgagc atactatttt ggctattatc
     4081 atcaggagaa aattgagcaa ctgtataatt tggattattt gtatcatatc gcttatcatt
     4141 cttatgatat gtgcgcattg gttgtagaga actactttca aaaccacctg caataattaa
     4201 atcacactga ccagatttaa cctttgaaaa agctatgtct atactcatca tagcagaagc
     4261 acactgcata tccacagtaa aagcaggtac ttcatttgac actcctgctg ttaaagtcat
     4321 taatctagct atattccctc ctgtcccaac tgcatttcca catataatct catcaatttt
     4381 gtcaatttca tactttttaa ttaaatcttt taaaacactt gctcctagta attcaggttg
     4441 aacaaattga aaaattccat tttttagacc tatatgactt ctcaatcctc ctaatataaa
     4501 aacttccttc atacattaca cccttcctta tattatagat ttatcataga tttcttctga
     4561 gtacattgtt cagtttagtg cctaaaaaag atgcaataac tgactttatt gtatctccaa
     4621 ttaaaaatgg tactactgct gcaaaaagtg ctgcttctac tgtcatttga ttaaaatagc
     4681 acataaatac tgttcccata aaatatataa taggcattcc aacaaaaatt gttattagta
     4741 tatatctctt aatatcattg tttcgtcctt ttaataaact aataattagt gctgcaaaca
     4801 aaaacccaaa ataaaaacca cctgttgggc ttaacaactt tccaatacca gatgtacctc
     4861 caccaaatac tggaatccca attaaaccta tcattatata tacaccaact gtactaaatg
     4921 cttgtcttgg tgttaaaatc aaagctatta aatttatcat tattgtttgt gctgttactc
     4981 ctgctggagt aaatggcaat ggtatatata tgtaagatga tatacacaat aatgctacac
     5041 atattgacat tttagttaaa tcttgaatat ttactttagt atttttcatt tttataattc
     5101 ccttcatatg tcttatttca tttagtttat atgtacatat acaaagcttc tattaaagct
     5161 ttgttttagt ataaaataaa gacgtttttt tgtcaactat tataattatt taggtttaca
     5221 tttaatttat aagtaaactt tatactgtta ctttatattt taatagtcat ttataaaact
     5281 ttgcttaaaa acagctctat ttagactata cttgtaaatt ataatatgat atatttttat
     5341 ataagatttt attttatata aaaaatagta acaaatagtt tatacacaag atatttaaaa
     5401 ggaagtgatg atatgaaagt taatgaaaat aatataaata atatagatga agagttaggt
     5461 gaacttagta ggaaagttta cttagagtcc ccagattttt ggataagtat ttctgacaaa
     5521 ataatagata aagtatttga tgaaatgtta ttattttttg cagttaaata taactatttc
     5581 aatatagtat gctttgctat tgaaaacaat tatctggatt taaacatgcc ttcaaaaaac
     5641 aaaaaataca ctaatataat ggagcatttg ttagatacat caaagcagaa tgaagataaa
     5701 aaaatttata attatcttct aagcttaaat aaaggtaata acctagatga aagaccaatt
     5761 ttagatgaat taaactgtaa tattagtagt gatacttttg aaaaaaaaga tatatatctt
     5821 ccaaagtact tatgtccaag ttgtaattct aatatatttg atacaggtta taaagtatca
     5881 aatgacataa catataaatt ttcttctaaa gaagctgaac ctattgaaat gtgtagtgaa
     5941 gtttcttcta tcagatgttg taattgtgat gaattattag aagatattac acttgataaa
     6001 ctatataacc tatgcaaaat tcaaaactgt aataattgtg gttctaattt aactaaggta
     6061 ggaattattg caaagaaaaa aatgaacttt gatgagaata ctgaaaaatt taaagatgtc
     6121 tcaacatctt actgttgtgg aaattgtgac tctgaaataa gcttatctca aagagaacat
     6181 tttaagattt aaatatttat aggctaaagt aataagatta cattattaga tactgtataa
     6241 gttttttaaa tctgtaaaaa taaatacact tataagaatt aaaattgcta tgaaactagt
     6301 tcttgatgaa ctaatttcat ggcaattttc tataatctaa taattattaa attttaacaa
     6361 acctttatta gcattccata attatttaaa attctttcaa ataccctata gtttataggt
     6421 gaaagtatct ttaatattat atcaaactta ttatctttaa atagtaaatt tgttaaatct
     6481 gaaatacacc atacaatttt attttc
//
LOCUS       DAEOSP010000189         6490 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.193, whole genome shotgun sequence.
ACCESSION   DAEOSP010000189 DAEOSP010000000
VERSION     DAEOSP010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.193"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     misc_binding    342..579
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            643..2562
                     /gene="thrS"
                     /locus_tag="KRH54_003025"
     CDS             643..2562
                     /gene="thrS"
                     /locus_tag="KRH54_003025"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBG8580660.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            3330..4421
                     /locus_tag="KRH54_003026"
     CDS             3330..4421
                     /locus_tag="KRH54_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG8580661.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPRLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSSLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
     gene            5087..6427
                     /locus_tag="KRH54_003027"
     CDS             5087..6427
                     /locus_tag="KRH54_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG8580662.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT         2559 a          604 c         1122 g         2205 t
ORIGIN      
        1 atagtattta agtatttact gtactactta ttaactatat taagtattga ttgtatttaa
       61 gtatacaaaa cctaaaaaga gtacaaaaat tagaagttag ttttaataaa aattagtttt
      121 agtatttatc tagtcatatc tatcatgcat gtggatacgt tttaaaaatt ttaaatttat
      181 gaaaatatta taatatcact ataaatcaag agattaattt aatataaaaa tataagaata
      241 ttggtttaca gctgattgag atgaaaaata atgggtaatt aatatattac attaatgttg
      301 attgatgtat aactatctgt tataatgaaa atatattaaa aatcatagtg ttgaagagga
      361 agagtagagt agaaaccatt ctcagagaat ttgggtctat ggctgagagc ccatgtaata
      421 ggtctatttg aaaactacct tggagctgag cttattagca gtatttgttt tgtactatac
      481 gtgaaagtgc tcgggtgacg accttatagt catcaagagg gttgtatata cttgtatacg
      541 ccaacttggg tggaaccgcg aattaatatc gtcccaaaca tatttggggc gattttttat
      601 tacaaaaaat ctaaaaatta tttcaataag gaggaaaaaa caatgattaa agttgcttta
      661 aaagatggct caataaaaga atttgaaaat gctatttctg ttatggatgt agctaaatcc
      721 attagtgaag gattagctag aaatgtggta gcagcgtctg tgaacggaga ggttgtaggt
      781 ctggaccaca ttatagatac tgattgtgac ttgaatttat ttaagtttga agataaagaa
      841 ggtaaggaag tttttagaca tacatcagcg catatattag ctcaagctat aaaaagactt
      901 tacccagaag caaagcttgc tataggacca agtatagaaa atggattcta ctatgatata
      961 gatttagacc atagattagt tccagaggac ctagagaaaa tagaagctga aatgaaaaaa
     1021 atagctaaag aagatttaaa aatagaaaga tttgaacttc ctagaaatga agctttagag
     1081 cttatgaaag aacaaggaga agactataag gtagaactta tatctgattt acctgaaagt
     1141 gaaataatat ctttctacaa gcaaggtgat tttactgatt tatgtagagg accacattta
     1201 ccatctacta aaaaggtaaa agctgttaaa ttacaaagtg tagcaggtgc atattggcgt
     1261 ggtgacgaaa ataataagat gcttcaaaga atatatggaa cttcttttga gaaaaacaaa
     1321 gacttagaag agtacttaca tttactagaa gaagctaaaa agagagacca cagaaaatta
     1381 ggtaaagaat taggattatt tatgatacct gaagaaggtc caggattccc tatgttttta
     1441 ccaaaaggta tggaacttaa aaatgaatta ttaaaattct ggagagaaat acacagaaaa
     1501 gctggatata ttgagataga atctccaatc atattaaata gaaaattatg ggagacttca
     1561 ggacactggt atcattacaa agaaaatatg tatacagtta aaatagatga tgaggattat
     1621 gctataaaac ctatgaactg tcctggtgga ttaatttact ataactctca actacattca
     1681 tatagagatt tcccaatgag agttgcagag cttggtagag ttcatagaca tgagttatct
     1741 ggtgcattac aaggattaat gagagttaga gcatttactc aagacgattc acatatattc
     1801 atgttaccag agcaaattaa ggacgaaata aaaggagtag ctaacttaat agatggtata
     1861 tacaaaactt ttggatttga atataattta gaattatcta caagaccaga gaactctatg
     1921 ggaagcgacg aagagtggga agcagcagaa aatggattaa gagaagcttt agaagagtta
     1981 ggtctaccat atactattaa tgagggtgat ggagcattct atggtcctaa gatagacttc
     2041 catctaaaag attgtttagg aagaacttgg caatgtggta ctatccaatt agatatgcaa
     2101 cttccaagac aatttgataa tacttatatt ggtcaagatg gtgaaaagca tagaccagtt
     2161 atgattcata gggttgcttt tggtagtata gaaagattta taggtatatt aatagagcat
     2221 tatgctggta aattcccagt ttggttatct ccaactcaag ttaagatact tcctatatct
     2281 gataaattta tggattatgc aaatgaagtt aagaaagagt tatttgataa gggtataaga
     2341 gttgaattag atgatagagc tgaaaaaatt ggatttaaga taagagaagc tcagcttgaa
     2401 aaagtgccat acatgttaat agtaggtgaa aaagaagtag ctgataataa tgtttctgtt
     2461 agatcaagag ataaaggtga aataggtagt attaaattag atgaatttat agctagtatt
     2521 tcaaaagaaa ttgaatcaag agaaagtatt attcaagact aatatatatt attttagtag
     2581 ctaagatatt gttatataag ataagtttaa tcaatatgtt gatatgttga tgcatagtga
     2641 ttaaatttat gaaaagatga gtataattag aggtacagtt aatcatattt ttatgattaa
     2701 tgtacctcta gttttttgta tggtagattt attaataaat atatcataga agtgttttga
     2761 acatggaaat atttatgtgc attctttaaa taaaagttta ttttttgagt gataattaag
     2821 tgcttgttgg taaggttatg gttgtaaaaa tcataacttt attttcatat ttgttccatt
     2881 ttcataaaaa ttattatccc tttaagaaaa tttcttaatc ctcttgaaat atttcctttt
     2941 tagtctaaag ttaaatttct ttcttcaaaa tgaatattta aatgaactaa aatttaagta
     3001 cattttctaa tcttatgagt ctatttttca tagataattg ccctataata gaagaatcta
     3061 tgttggagct atgtggtgac aataacagta ttgtcagtga ttaattaatg ctttataaat
     3121 gattaattag tgttttatgt tatttttgga cttaccctct atacatagat taatttattt
     3181 aactttagat agagagagaa tgtcaagtac atacacagac attagagttt aagaaagtgt
     3241 ttatggagca agaactttta aaagaggtag tgacttagtt taatttcaag aaatgataaa
     3301 tacaaaagag gaaaaagaga ggtgttgaaa tgatagaaaa agtagaagaa aaatctaaga
     3361 ttattaaaac atcaaaagaa tataagcgaa ctataattga agaaaaagat aaagaatgga
     3421 ccaaaaaatt aaatgatata cttagtagaa gtagtttttc taatgatgaa ttagataaaa
     3481 tcaaggcgtt aattccaaaa gagattttga ctaatgaaaa tgttggagaa gttgtgactg
     3541 aagatggata tgctaagccc gagtatgatg aggtatttaa atcattgttc actttaaata
     3601 atgaatatga tttacttgta aattttataa atgatatcct aaaagatgct ccatatgcta
     3661 acaggaatat caaaccgttc actcaaataa agagaataat tagagttgaa actgacccaa
     3721 caattaatta cattggagag aaaagaccta gacttgatat tttggctgaa gatgaagaaa
     3781 ataatcatat aaatattgag atgcaaagag cttttgagaa cgattatttg gaaagagctg
     3841 aatattatct tagtcgtgta catggtagaa agttagaaga aggtaaagag tataaagaaa
     3901 ttggaaaaac tattggaata catatactaa atcatgtaaa atataatcac atagaagatt
     3961 atgtaaattg cctgagatta acaatggacg ggcatccaga tatttattct agtaaaactg
     4021 cattatattt tatagagctt ccaaaaattc ataaatctga ctatatagta aatagaatta
     4081 tgctatgggg aaaacttatt agtaatcctt ctagcttaga tgtaagagtt atagctaaga
     4141 atgattctat tattaaaaaa gcacttgata gattaaaaga attaggtagc aatgaagaat
     4201 atttattaaa tctgaaaaga ggtgcttata tcatgaataa aagtagaaat tttaaagaag
     4261 agattgaaag agagacagca attagagttg caaaagaaaa agattataaa ataattataa
     4321 tgttgaaaaa aaatggtttt aaattagatg acatattaaa taaatttaat gattttgact
     4381 taagtgaaga tgagataaaa gaagtatatg aaaataattg atgataaaaa gtgaacttat
     4441 ataagtttgc tgaatcggag tctggaaata tttatgtgta ttttttaatg aataaaaatt
     4501 tattttaaaa acaataattt agaggtacag ttaattatat ttttatgata ctgtacttct
     4561 agttttttct attgtagatt tattatttac taataaatct acaatagaag tgcttttgtt
     4621 gtaagaatca ttttatctaa tgattgtttt ttataatagt taaatttcca tttgtagtgt
     4681 ttaaatataa atctataaca gcatttctat aaatgttata aatcactata atgattcaac
     4741 tcaaagttac tgcttaaaga agcagagatt ttatcaaggt atatcatgga aaaaaatatt
     4801 ttattgtaat atttataaga ttttattatg gaaataatgt aaaaacttta taaataatat
     4861 attttatacc tagttataga taaatttata tagttattat taaaaaaagc aataaaaagt
     4921 ttaattcaat ttagcaaatt tagtgatata attaaaaaag aaaagaaata tggacttatt
     4981 tggaataatt ttgacaatct atttactaca cgtgtagagt cgtatataat taaattatac
     5041 ataaaatata aaaaaataat ttagtaatac gttaggagaa agtattatga taaagtcaag
     5101 cttattttgg aatattttca ttgcgatatc ctttatgata gaatggtttc tttgtaagaa
     5161 aatgctagat tatacaagtg agaaaaggat ttctaacaaa aaaataaact tttgtatggt
     5221 aggtattatt gtttttgctg acataatata tttttttgat gtacctccaa atataagagt
     5281 aattatatct atgataatta ctattgcatt ttataaaatc tcatatgatg tggggtttat
     5341 aaagtctgta ttaatatcct taatttattg gatgttacta atcgccacag atgctttgag
     5401 tatatcttta acagtttggc ttaattctat aaatgacatg agtagtatgg ggacgggaga
     5461 tatatacaga attgagtcta taatattggg aaagatactt ttgattgtgt ctttgctttt
     5521 atatagagcc ttaaagataa aacttaatat agaaataaat aaaaggacat tattttatat
     5581 agttattcct atagtcgcta atatagcatc tttttttgta atttttgaat acatatttaa
     5641 tccagggtct ggtagcttaa tacataattt ccaattcgtt atgatttctg ttgtattgct
     5701 tatatcaagt atcactttag ttttaattgc taaaaaatta cagcaatata atactttagt
     5761 tgtagaaaac aatatgatta aaaatacttt gaagtatgaa aatgagtatt atgaagatat
     5821 agaaaagaaa tatttaaaaa ctagattact aagtcatgat atgaaaaatc acatattgtg
     5881 tataaatgct atgattaaaa aaggtattga tgttacttct tatttaaata atttgcaggt
     5941 agaaattcag agtaatgact tgttatttaa tactggtaat tttatattag atgctatatt
     6001 aagtgaaaag aaagaggttt gtgataagaa tgatatagaa ctaaaaatag gaataaattt
     6061 tagttgttgt gaagctgttg aattagtgga tatatgtcat atattttcaa atattttaga
     6121 caatgcaatt gaggcatgta ataaaattga tagtggaaat agagttattt gtgttaaagg
     6181 tgatgttatg caaaattttt atttaataag agttgaaaat actaagatga ataagattat
     6241 tactaaaaat ggagatattt tgacagacaa aaaaggttct cttcatggac ttggaataaa
     6301 aagtgtaaaa aactctgtaa ataagtatgg tggtaatgtt gtaattgaat atgatgataa
     6361 aaaatttgta gttaaaattg ctattccact tagaaatgtg aagaaagcag ttaatcatgt
     6421 gatttaaatg tatataaatt tgatgtgtag aaatttaata tataagaatt aatgtataaa
     6481 agtgatgcat
//
LOCUS       DAEOSP010000190         6472 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.194, whole genome shotgun sequence.
ACCESSION   DAEOSP010000190 DAEOSP010000000
VERSION     DAEOSP010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.194"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(100..708)
                     /locus_tag="KRH54_003028"
     CDS             complement(100..708)
                     /locus_tag="KRH54_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBG8580663.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(817..3555)
                     /locus_tag="KRH54_003029"
     CDS             complement(817..3555)
                     /locus_tag="KRH54_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBG8580664.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLKDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3703..4494)
                     /locus_tag="KRH54_003030"
     CDS             complement(3703..4494)
                     /locus_tag="KRH54_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBG8580665.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4530..5186)
                     /locus_tag="KRH54_003031"
     CDS             complement(4530..5186)
                     /locus_tag="KRH54_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8580666.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5179..6144)
                     /locus_tag="KRH54_003032"
     CDS             complement(5179..6144)
                     /locus_tag="KRH54_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBG8580667.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6250..6356)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         2046 a         1093 c          592 g         2741 t
ORIGIN      
        1 tataaagtta tttttatcaa actaaaaatt ctataaaaaa ataagataca tacctataaa
       61 ataccttcct atagatacat atcttaccta tataaaaact tattctatat aaaaacgcca
      121 taaaaaatct tttgcttctt ctgcataatc tatatttata cgtttactag tttttatatc
      181 taactcatct ttactaaata ctttttttcc tttatcatca taataaaaat cacttatata
      241 taattcttca cccattatac tcttactatt tagacttcta tctattttta aagctttgca
      301 aagttttcct ggtccgttag taatattttt tacttgatat ttactcaact cagtgtaagc
      361 tttgttgtat ctattcatag atatctcatc taaagaagtt ataggctcta cccctcttat
      421 taatacacac tctggcacat tttctatatt tgcagataaa ttcaagcaat tatacatacc
      481 ataaattaaa taaacatata tatgtccacc atctaaatat aatggcttag ttctatctgt
      541 ctttttattg ccatatacat gtgctccttt atcatttaca cccatgtatg cttctgtctc
      601 tatgattttg gtaacaatta ctttattttc atatttcctt attagatatt tacctaatat
      661 tgatttggct aaatcaattc cattttttct aaaaaaatcc ttttccaaaa tttcacctca
      721 taaaatttat ttaaaacgca tcaaatacaa ctaaagtaat aaataaaatt aactcttatt
      781 aatgtgatat atataaacaa taaatttaga catttattat ttatatatat caaaaaattc
      841 tatctcaaac ttttcaatat tatcattgta atttgaatta tcatatactt cttcaacaac
      901 ctctttaatt gggaatataa cttttacatt agttccttct cctaccttac tatctatgta
      961 aatcttccca ttttgaattt caatcaatga ttttgtaagg cataaaccta taccacttcc
     1021 aaattcattt gatataccac tgttttcaat ttgtttaaat cgattaaata tatcctttaa
     1081 tttttctttg tctatgccca ctccagtatc ttttataatt atctctactg tttcgtcttt
     1141 tttatatata cctacaagga tttctcctcc ttctaaagta aattttatag catttgataa
     1201 tatatttagt attattcttt ctatcgattc tgggtcaagc cctaccataa cagtttcttc
     1261 atctgtatca aaaattaaat ctattccttt agacttagca taactactaa tagatgtaac
     1321 tattctttct gttatgtcaa taatatcaaa attaactgct ttaatcttat atatgtctga
     1381 cttaacttga gaaatattta ttatattatc tattaatctc aataatctaa acatattttg
     1441 tttagttatt tttatatgtt cttttagtat atcctgaaaa tcaattattt ttcctttttt
     1501 ataaaggttt tcaataagtt gaactgaact atatatatta tttaaaggtg tcttaagttc
     1561 atgagatata ttagcataaa actccatctt catttggtca tactgtcttt gttttattaa
     1621 tgctttttta agtctttcag actctctttg cacacttaaa tctttaataa ttctcactct
     1681 acttcttttc tgtccaaagt ataatgttct tgatatcatt tttacttctt taatgtgatt
     1741 attttcattt actatatgga tactcttctc tctttctcct atatttaaat tgtttatgta
     1801 tttttttact aatatatttc tatatttttg tgttgtaact cctaatacat cactagaaat
     1861 tccgaacaac tctctaaagg ctctatttgt atatagtact cttgatgtac ttaaatcctc
     1921 aagaaaaata gcctctggta taacatccag taatttttca tatattattt tttgttcatt
     1981 tactgttcta agaatattta tctctttagt tatatctcta aatgtaagca atttaaagag
     2041 tttattatct gaccaatagt ctccaactct aacttcttca acatgttctt caccttctga
     2101 gtcagacatc acaaaattaa ctatacagtt tgctgtgtca ttgtaactta ctatttcagc
     2161 tttcttttct tgtgttagat ttaatctttt aaattcccta ttgtcaaaga aatctatgtt
     2221 aaaagtttca aaatattgtt ctaatgtcat atcgactaaa tcacttgaaa cacatttaaa
     2281 actcttttca tattttttat ttatacttat taccttattc ttctttatgc caccttcagc
     2341 acttcttaca agtataggag atggaatatt tagtataatg ttttcatagt ctttatttat
     2401 atttcttatt tcttcatcta ttttgatttc atttgtaata tcatttaaac ttattaagta
     2461 attgctttcg tctttattat tacttaaact taacaaataa attttatcat ttaactctac
     2521 cagtttatca attatactta tatcgttatc aaatttcttc aatttttcag caactttttc
     2581 atcttccttt ataaaagtta atattttata ataattattc atatcctttg aaaattcatc
     2641 tttaaattta ttatttgtgt aacttatatt aaaatcatag tctgttacta tccatacatt
     2701 tggtattgta tccagagctt gtctaagtaa ttcttttctc tggtctatgt agttttttaa
     2761 tgttctttgt ataatcatat ccttttctat tgctaaattt atctttccta attgctctga
     2821 ctttttactt aattttttat tgattgaatc aagttcataa tatggataca ctactctatt
     2881 attaagactg tatacatata cataataagt atggttacat aatagtaaat atattactcc
     2941 tattaatcta tctgaatcta ctatattaat cttagaaatt gctacaaaac atataatagt
     3001 tgtaaaaaaa acaaaatatg ttaaatcttt tattaactta tagtgctcaa tgttactatc
     3061 aagtttattc aacaaatata gtaaattaaa catagatata caaatattaa ttaatataat
     3121 aatcatattc cagttaatat cactaaaaat atttcttata aagtaatcta atatcacaaa
     3181 agcactataa aaaattagta ttgggcttat tcttagtttt tttactttag aaatggttaa
     3241 gtatataaaa gtagacatat taattatgct taagttaagc aatacaattt ggaatttttg
     3301 aatttttaaa tttactactc ctaaaatact tacaaaaact aatacaagac accctacttt
     3361 caatatattt ataaattcat ctgttttaaa gctacttata taaactatag aatatccaaa
     3421 tatccaaagt aaaattattc tatacatatt tatagaaact gtatacaaat ttatatttaa
     3481 aagtgcaata accaaaaata acccattagc tagtatatag accttattct tacttaaagt
     3541 tcctttagat tccatattcc ccaccacttc ttcattttta tatagtttta cctataaata
     3601 tgttaccaca attcttccaa aaaatttgtc actttctagt tataaaaaaa aatgagtaaa
     3661 tatttttaat tttactcatt ttgtagattt tgtatattca gtttaaaaag ctggaactat
     3721 acttccttta tattcatctt gtataaattt tttaacttct ttactattca tagcctctga
     3781 taaagcctta atcttatcac tatctttatt attttctcta caagctatta tatttacata
     3841 tggagaatca cttgattcta ttactattgc atccttagta ggatttaagt tagctgttaa
     3901 agcataatta gaatttatta ctgctccgtc aacatctttt aatactgttg gtaattgctc
     3961 tgcattcatt tcttttatta ctatattttt aggattttta gttatatctt ttttagttat
     4021 aagctctcca tcttttactt ctatcaattt attttttgct aataatttaa gtgctctagc
     4081 tccattagtt gcatcatttg gcacagctat aacagctcca tcttttaatt catctaatga
     4141 cttaactttt tctgaataga atcccattgg ctctatatga actttagatg tataagtaag
     4201 tttatatcct ttctccttca cagtttcctc taaaaatgga atatgttgaa agaagttagc
     4261 atccaaagac ccttcatcta aagctgtatt tggaagtaca taatcatcaa atatttttac
     4321 ttctaaatca tatccttttt cttttaacaa aggttttgca acttccaata tctttgcatg
     4381 tggatttgat gaagctccta ctagtatttt tttatcctct ttgtttgaac aacctactgc
     4441 tgatattgct attgctgaca ccaatgctac tgataataat ttctttaatt tcatttttac
     4501 tccccctctt taaaatgttt ataattaatt tattttagtt tcttataagc cagatttcct
     4561 actccctgga ttatctgaac taatattata agtgctatta cagtatatac cattactgct
     4621 gtatcaaatc tttgatatcc ataaataagt gctatattac ctaatcctcc agctcctact
     4681 gcacctgcca tagctgtata accaagtata gatattattg ataatgtaat tccagatact
     4741 attgaaggca tagcttcctt tatcataact ttaaatacta tttgtctttt tgtagctcca
     4801 aaagattttg ctgcttctat caatccttta tcaacttcat ttaatgagct ttctataatt
     4861 ctagcgataa acggtgctgc cccaatagtt attggcacta ttgcagcagt ttcccctata
     4921 cttgttccaa cgattaatct agttattgga atcattgcaa ctataagtat cataaatggg
     4981 aaacttctaa atatatttac tataaatcct aatgcactat ttattgtgct atttggtttt
     5041 aatccatctg gttttgtaac tactaatatg attgctaata taaatcctaa tattgttgcc
     5101 actatagttg gtactataac catatacaaa gtttgtaaca atgcatttgg aaatagtgtt
     5161 gttaaaaagt caattaaact attcatttat catttcctcc catcttatgc ccttactagt
     5221 tagatattgt tttacttgct ctccactttt atctgaaatg tttataataa gtgaacctaa
     5281 aatatcatct ttaaattgtt ctaatttccc aaatattata gacacatcta tatttagttc
     5341 tcttgccata gtcgtaataa tagcttcatt agaaatatct ttagggaata gtatctttat
     5401 atttgttcct tttggcaata ttatactttc ttcacctatt aattttctaa gtcctttagt
     5461 atttcttaag aatatttctt ctgtatctcc tacctctaat acttctccat tttccattat
     5521 tgctactctt ccacaaatct gctttataac ttccatttga tgagttacaa ctattattgt
     5581 tattcccaat tttttattta tatcttcaag caatgaaaga attgactttg tagtatttgg
     5641 gtctaatgct gaagtcgctt catcacaaag aagtacttgt ggatttagtg ctaaagctct
     5701 agcaattgca accctttgtt tttgtccgcc acttagattt ctgggtttat catttttctt
     5761 ttcagatatt ccaactactt ctaataactc taaaactctt tttttaattt ctgcctttga
     5821 gtatccaaaa cattctaatg gcaatgctac attatcaaat acagtttttc tttcaagcaa
     5881 tgaaaagtgt tgaaaaatca ttcctaactc ttttcttaaa tctctcattt gcttttcatt
     5941 caatgacttt acttctttat cagaaacaag tacactgcct tcttggtatt cttctaatcc
     6001 gtttatacat ctcagtagtg ttgatttccc tgcaccacta tgacctatta ttccaaaaat
     6061 ttctccagat tctatttcaa tacttacatc tttaagcact tgaatctttc cataatactt
     6121 attaacattt tttatgctaa tcataaaatt tcctcccata tattctgctc agtttttatg
     6181 tttatcctct cttatgtatt acaacaaaat aaaagcccga gtataaactc aggcttagaa
     6241 agctatttat actcatctat caactatatt agttgcagga tttagcacca atgtatataa
     6301 aatatactgg ttgccgggtt tcaaagggcc agtccctcca ccactcttga taagacgtta
     6361 tttaatttaa ttctattatc atttgttgct atgttatata tgatatattc attaatagta
     6421 tttgtcaata aatttattta aattttttat tttttattat cgttaattaa ct
//
LOCUS       DAEOSP010000191         6377 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.195, whole genome shotgun sequence.
ACCESSION   DAEOSP010000191 DAEOSP010000000
VERSION     DAEOSP010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.195"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            302..1246
                     /locus_tag="KRH54_003033"
     CDS             302..1246
                     /locus_tag="KRH54_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBG8580668.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1268..1894
                     /locus_tag="KRH54_003034"
     CDS             1268..1894
                     /locus_tag="KRH54_003034"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBG8580669.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1928..2977
                     /gene="hisC"
                     /locus_tag="KRH54_003035"
     CDS             1928..2977
                     /gene="hisC"
                     /locus_tag="KRH54_003035"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBG8580670.1"
                     /translation="MREKESIRELKGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYK
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2980..3558
                     /gene="hisB"
                     /locus_tag="KRH54_003036"
     CDS             2980..3558
                     /gene="hisB"
                     /locus_tag="KRH54_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBG8580671.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3560..4165
                     /gene="hisH"
                     /locus_tag="KRH54_003037"
     CDS             3560..4165
                     /gene="hisH"
                     /locus_tag="KRH54_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBG8580672.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4162..4884
                     /gene="hisA"
                     /locus_tag="KRH54_003038"
     CDS             4162..4884
                     /gene="hisA"
                     /locus_tag="KRH54_003038"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBG8580673.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4866..5624
                     /gene="hisF"
                     /locus_tag="KRH54_003039"
     CDS             4866..5624
                     /gene="hisF"
                     /locus_tag="KRH54_003039"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBG8580674.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5652..6320
                     /locus_tag="KRH54_003040"
     CDS             5652..6320
                     /locus_tag="KRH54_003040"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBG8580675.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2608 a          540 c         1260 g         1969 t
ORIGIN      
        1 caaaaaactg taaatttaga atattaataa aaattatttg ggaggaaatt atcatggtga
       61 gattaaatat agtaaaatta aatacaaaat taaatcatca ccatcatcat agattaaagg
      121 gattgtaata tgaaattaat tcatagacac aagccctgtt tttagtgtaa ttggtcttgt
      181 atctatgatg ggaagtaatt ttaagagaaa attatagcca tataggtata aagcctatgt
      241 ggctttttta ttgttttaaa tttgcaaata taagagtaaa tttaatgaag agggtgagaa
      301 tgtgaaattt ttgaggattg aagatgaggt tatttattct aaaaaaagat ataaattaaa
      361 aagggaaata gataaacttt ttttggatga agaatatttt caaatagaac cacaattatt
      421 tgaagaatat gatgaattta caactataaa taataaaatt ccagaagaat ctatggtaaa
      481 ggttgtaaat ggaaaagtaa tggtacttag agctgatata actacaagta taataaaaag
      541 ccttgtacca agatgggaag atggtttaaa gcttaagctt ttttataatt catcaatata
      601 taaaaataaa aatacagttg gaataaaaga gataagacaa ataggttgtg agtatctagg
      661 agaagcctct gtagaagcag atagagaagt agtgaaatta gctcttaaga tacttgaaaa
      721 atacaataat aattttattc tagaggttgg gagtagcaag tatattcatg gtttgttaga
      781 agaattaaat ttaaataaga attgtgaaaa tcaaattaaa aatttactat atacgaagaa
      841 cactcatgaa ctaaaagtat atattgaaga attaaaaatt aagcgtgaag taaaagagtt
      901 attatcaaat attttaagtt tacaagggaa tttatataat gttattgaaa aatcaaacaa
      961 gttctattgt aataataaga tgaagcaagc tcttgaagaa ttaaagcaag taaataattt
     1021 gattgaggaa tgtaattttt tagataaggc tagatttgat ttatcaatga ttactatgct
     1081 tgactattat gaaggaatta tgtttagagg atactaccca aactcatata aagaaattct
     1141 tagtggtgga agatatgatt ctttaactaa agagtatgga aaagaaattc cagccattgg
     1201 atttacacta agtgttgatg agttaatgaa gtatgtatat aagtaaagtt tagaaggagg
     1261 gattgctttg gattatttga ctatagcact agcaaaagga cgaattgaag gggaatcatt
     1321 taaaaaattt aaaaaaatgg gtctaggaga cagtatagac acagatacga gaaaactaat
     1381 ttttaaagat gaagaaaata aaattatata tatacatgta aagccatcag atgtagttac
     1441 atatgttgaa aaaggagtcg ctgacctcgg tatagcaggt aaagatacta ttttagagaa
     1501 tgagacagat gtatatgaaa tatatgattt aggttttgga aaatgtaaat ttgctgtagc
     1561 aggattaaag ggagatagca tatatagaga agatgaatat ttaaaagtgg caacaaagta
     1621 tcccaatata gcaaaaaagt attttaaaga gaaaggtcaa aaaattgaaa taataaaatt
     1681 aaatggttct gtagaactag ctccaatagt agggttgtca gatgtaattg tagatatagt
     1741 tgagactggc aatacactta aagcaaatgg tcttgaaata ttagaagaca tatgtaatat
     1801 aagtgctaga attatatcaa atagagctag ctatagattt aaatatgaac aaattcagaa
     1861 tataattcgt ttatttgaag aacttgataa ttaaaatata attgagcttt ataaggaggg
     1921 atttaagttg agagaaaaag aaagcataag agagttaaag ggatatgaac caaatcacgt
     1981 aaactgtaaa gtaaaacttg atgcaaatga aggaagtaag aggttgttta aataccttat
     2041 taaagaaatt tcagatagtg atatagattt aaatttatat cctgaagatt cttatagtga
     2101 tttgaaagaa tctataatag attacataaa tatttctgga gtaaataaga aaaatcttct
     2161 agtaggaaac ggttcaagtg aaataattga tttaataata catacttttg tagataaaga
     2221 tgaagtaata ttgagtttct ctccaagttt tagtatgtat tctatatata gtcagataaa
     2281 tgggtctaaa tttataggtg ttgaaagtga tgaaaattta gttataaata tagataatgt
     2341 gattgaaaaa gtaaaggaga ataatcctaa aatagtaatt gtatgtaatc ctaataatcc
     2401 aactggaaca atattaaaga gggaagagat aataaagctt ttagattcaa ctaatagtct
     2461 agtggtttta gatgaagcat atatggattt tggagaagaa agcatgttaa gtgatgtttt
     2521 taagtatgat aatctaatag ttcttagaac tttatcaaag gcatttggat tggctggaat
     2581 aagaacagga tatatgttat ctaatagtag tttaataaat tctgttgaga aagtaagacc
     2641 tccatataat ttaaattcat tatcagactt tattgctaca agagcactta gaaataaaga
     2701 tgtggtaaag gcttatataa aggaagtaaa agaggaaaga gaagttttat ataaagaaat
     2761 gattggtatg ggaattaaag catataaatc acaagcaaat tttatattat tttattctga
     2821 aatcgaaaat ttatcacaaa aacttattga tagaggtgtt ttaataagaa aatttggtgg
     2881 aaagttagaa aattattaca gagttacaat tggtgataaa gaagaaaatt ctatgtttgt
     2941 tggagctata agagatattt tgaaaaagga gaaataggta tgagaatttg gaaggtagaa
     3001 agaaatactc ttgaaacaca gattttagta gaattaaata ttgatggttc tggtaaagct
     3061 gaaatagata ctggaatagg atttttagac catatgctta ccttaatgtc atttcatggc
     3121 aagttcgatt taaaagtgat ttgtaagggt gatacatatg tagatgacca ccatagtgta
     3181 gaagatatag gtatagctat aggtgaagca tttaaaaatg ctctaggtga caaaaaagga
     3241 atacgaagat attctaatat ctatatacct atggatgaat ctctatcaat ggtagcaata
     3301 gatataagca atagacctta tcttgtattt aatgctaaat ttgataccca aatgattggt
     3361 agtatgagta ctcaatgctt taaagaattt tttagagctt ttgtaaatga atcgagagta
     3421 acattacata tcaatctctt atatggggaa aatgaccatc ataagataga aagtattttt
     3481 aaagcatttg caagagcttt aaaagagggt agtgaaatag tttctaatga gatagcatct
     3541 tcaaaggggg ttttgtagaa tgaatataat agtagactat ggtcttggaa atatagattc
     3601 tgtgtctagg ggttttagaa aagctggaat agaaactaaa atttctagtg atatagatga
     3661 aataaaacag gcagattcac ttatacttcc aggtgttgga gcttttagag attctataag
     3721 tgccttagat aaactaggat tgataccaat aataaaggag catgtttcta agggtaaatt
     3781 tatgataggt atatgtctag gaatgcaatt actttatgaa aaaagttatg agtatggaga
     3841 atatgaagga ttaggactta taaaaggaag tattgataaa ctggatataa gtttaaaagt
     3901 gccacatatg ggatggaata atctaaaatt taataaggca aatgatgata tacttaaata
     3961 tataaatgaa gatgattatg tatacttcgt tcactcatat tatgcaaatt catcaaatga
     4021 agaactaatt gcattttcag aatatgaaaa gaaaatccct gctattgtta gaaaaggcaa
     4081 tgtatatggt atacaatttc atcctgaaaa aagtggagaa gtagggttaa atatattaag
     4141 agcatatggg gagatgataa aatgataata tttccagcaa tagatataaa agataataag
     4201 tgtgtaagac tcacacaagg agaatttgac aaagtaaatg tatattatga taatccatta
     4261 gaagtagcat ataaatggaa aaatgaagga gcagaatata tacatatagt agatttaaat
     4321 ggtgctagaa gtgaatttgg tgtaaataca aaaataattg aggacatagc aaacaatata
     4381 gatataccta ttcaagttgg cggtggagtt agggataagg aaaaagtcaa aagtttaata
     4441 aatgctggtg ttactagagt aatattaggc agtatagcga ttgaaaattt gaatttagtt
     4501 gaagaattgg tcaatgaata taaagagaaa attgtagttt ctatagatgc taaagatgga
     4561 aaagtagcag ttaggggatg ggaagttgta agcaatgtag actcattaac attatgtaaa
     4621 caacttgaaa agataggtgt acaaactatt gtttatacag atatatcaaa agatgggatg
     4681 ttgcaaggtc caaattttga catatatgaa agaatagcaa aggaaacatc actaaatgtt
     4741 attgcttctg gaggcgtaac ttctatagaa gacgtaaaaa gactaaaagc tatgaactta
     4801 tatggtgcaa taataggtaa agcgctttat gataaaaaga tagactttaa ggaggcacaa
     4861 caattatgct tactaggaga ataatacctt gtctagatgt gagaaatgga agagttgtaa
     4921 aggggaaaaa atttaaagat atagtagatg ttgatagtcc agaagtactt ggaaaatttt
     4981 atagtgattg tggtgcagat gaacttgtat tctatgatat aactgcatca aatgaagaga
     5041 gaaagacatc tttagaattt gtgacaaaag ttgcagaaaa tataaatata ccattttgtg
     5101 ttggtggagg agtaaataaa ttagaagatt tcactgatat tttaagaaaa ggtgctgata
     5161 aagtttctat aaattcatca gcagtcaaaa atcctgaatt aataagggaa gcttcattaa
     5221 aatttggagc acaatgtgtt gttttatcga tagatgcaaa gaaaaatgaa gagggttcat
     5281 ggagcgtata cgtaaaaggt ggaagagaaa aaactaatct ggatgcaatt gaatgggctg
     5341 ttaaaggtgt agaacttgga gcaggagaaa tcgttgtaaa tagtatggat gaagatggaa
     5401 tgaaaaatgg atatgatata gaattattat caaagataac ttctttagta aatgtacctg
     5461 ttatagcatc tggaggcgct ggaaaaaagg aagattttta tgaggcggtt aataaatcca
     5521 atgtagatgg aattttagca gcatctgttt ttcattttgg cgagataaaa ataaacgatt
     5581 taaagaaata tttaaaagac atgggtgtag aagtaagatt ataagttaaa tactaaaagg
     5641 agattagaaa tatggataat aagtgtaaca atgtatacag tgatgaagta gagaaattta
     5701 taagaagtat aaagtttgat gataaagggt tagtccctgt tgttgttcaa gaggtagtta
     5761 gcaaagatgt actgatgtta gcatatatga ataaagatgc tataaagaaa accttaaaag
     5821 ataaagtagc ttgctatttt agtagaagta gacaagaact ttgggttaaa ggtgagacat
     5881 ctggtaatac tcaaaaagta gttaagatgt cttatgattg tgatgtagat actatactac
     5941 tttttgttga acaaactggt gtagcttgtc atacaggcaa ttattcttgt ttttacagag
     6001 acttgtttga tgatagttca aaaatggagc ttgaagtaca aacaaatata ttgaaagaat
     6061 tatatgattt aatcaatgaa agaaaaaata atccagttga aggttcttat actaattatc
     6121 tttttgaaaa aggaatagat aagattctta aaaaagtagg tgaagagagt agtgaggtaa
     6181 ttatagcatc aaaaaatacc gataaaagtg aacttattta cgaaattagc gatttagtat
     6241 atcatacatt ggttttaatg atagaaaaag gtgttgaaat tgatgaaata aaaaaagaat
     6301 tacttaaaag aagaaaatag aataagatag attaatgggt aataagaaaa tataccgctt
     6361 ttaataaaaa gtggtat
//
LOCUS       DAEOSP010000192         6322 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.196, whole genome shotgun sequence.
ACCESSION   DAEOSP010000192 DAEOSP010000000
VERSION     DAEOSP010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.196"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            141..3320
                     /locus_tag="KRH54_003041"
     CDS             141..3320
                     /locus_tag="KRH54_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG8580676.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTHQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNIHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            complement(3605..4369)
                     /locus_tag="KRH54_003042"
     CDS             complement(3605..4369)
                     /locus_tag="KRH54_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8580677.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4525..4758)
                     /locus_tag="KRH54_003043"
     CDS             complement(4525..4758)
                     /locus_tag="KRH54_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580678.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            complement(4832..6286)
                     /locus_tag="KRH54_003044"
     CDS             complement(4832..6286)
                     /locus_tag="KRH54_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG8580679.1"
                     /translation="MLKSLKSLGYNIPSRVQREVIPKLLKGQNLVVRSKTGSGKTASF
                     AIPLCENINVDYNNVQALIVVPTRELALQVKDEISDIGRLKKVRCSAIFGKQSIKDQI
                     AELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNKGFVEQMEKILLNL
                     PKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTRQIDDKIIKANGRE
                     KYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELHADLSQERRIFVIK
                     DFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIGRTGRKGNSGKAIT
                     IVTEKDEKYIENIETYIGYKINELKDIEQSRITHGKVLFEEYSKGVLKEVSKRKKVDK
                     NSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGITNDDIGVIEVQDLC
                     SYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
BASE COUNT         2348 a          898 c          741 g         2335 t
ORIGIN      
        1 atctaatagt aaattgttat ttacacatat attgtgatag ataaaagcta ttcatatctg
       61 ccttaatttt agtcttaaat ttagagtgta aattgaatgc atttagatta taatatatac
      121 agtgaggtaa ggtgaataat atgaaaatag atatgatata taaactaata agaagaaata
      181 cagaaccatc aagatggtca agaggtgtaa aatattatac tcatcaatta gttgattata
      241 tagacataga tattgatgac ctacaaaata acctgttgac atttcgtggt ggagtcaatt
      301 ctgaagataa ttatagcttt tattttaatt caataactat agatataaag cactttaaga
      361 taattgaaaa tgattgtagt tgcgctgact gtcaatcaaa gggcaaaagt atttttatat
      421 gtaagcatat agttgctatt gtgataaaaa gcttaaaaca aataaaaaat aaaaaagatg
      481 ttcctcctat tctaatagat atattaggtg cagataagga taagctttct aatacaaatt
      541 ctaaaaccag cttagattta agcaagcaat tactttcttt gttagacaat actaaaagag
      601 aaagggttaa tttagatgta ttgataaatg tggattcaaa taaagcaatt gctgaattta
      661 aaataggaat tgataaaatg tacattttaa aaaatattac tgaatttgca tattcaaaac
      721 ttaatggata tgaattagat tttgggaaga aatttactta caaccccaac attcattatt
      781 ttgctgatga agatgagaag ttagtagata ttatagaaga atatgggaca aatctttcat
      841 acaacttttc ttcatcacat tcaaaaaaat ttatggagat aaatgcaagc tctttaaaac
      901 aatttttaaa tgctattaag tacaaggatt ttacccttat tttttctaaa gaggaatatc
      961 atccaaagat attaaatgat actctacctg taaggtttga ttttaaaaat atagaagata
     1021 aaatagtctt atattctaaa gattcactac ctgttccact tactaaaaaa aatgatgtac
     1081 ttttatatga tggcaatatc tatttattat cacataaaaa agctcatgat tattcaaaaa
     1141 tatatcaaat attatcaaag actaaagagt taaaatttga taaagaggat tctaaggatg
     1201 ttttaggatt gctagttcct agattaaaat ctatatctca agaagttcat ttagatgata
     1261 acataaaaaa taatataaca aaagatttta aatctgagtt ctactttgat atgattgatg
     1321 gcaacatatg ttgtgatgta aagtatatat atgatgatga agataaaaaa tttgtgctcc
     1381 ccaacataca aaaggaagct actattgaaa ataaacttac ttcttcacaa tttacaaagg
     1441 aaaataatca ttatgtgttt aaaggaactg accatgacct atttacattt ttagatttac
     1501 aattagaatc tctaaaggaa tttggagata tatattattc tgagaagttt aaattgaaaa
     1561 aaatatataa cgcttcatca attcaagctt caatcaatca gactaaccag aattacctag
     1621 aatttaattt taatatatct gatataaatc caaaagagta caaggatatt ttgaaagctt
     1681 ttcaagaaaa acgtactttt tataaactta aagatggaaa ctttatagat ttatcagaaa
     1741 gagaaacaaa agattttttt gaattagttg aaaatctaga tataatggat aaatcaaaaa
     1801 ataataaaat tcataaaaat aaagctctat atataaatga tgtaatacgt agtaaaaatt
     1861 taaagtttat agatggtaaa aaagaactag acagtatatg tgaaaaattt agaaattttg
     1921 atagcataaa tttagaaatt cccgcaaatt taaatgcaaa ccttcgtgac tatcaaataa
     1981 atggcctcaa ttggtttaaa gttttagatt attataaatt tggtggtata ttagctgatg
     2041 aaatgggtct tggtaaaaca atacagacta ttgcattttt attatcatta tctaataaaa
     2101 aaagtcttat tgttacaccg acatcactca tttataattg gaaaaatgaa tttgaaaagt
     2161 ttgctccaga tataaaagtt ttattgatac atggaaataa aagagataga gaaaaatgtt
     2221 ttatggaatt agaaaatttt gatgtaatac ttacaacata tggtactctt agaaatgatt
     2281 tagaaaaata tagtgaaata aagtttgatt attgtatatt agacgaagct caaaatataa
     2341 aaaatccagt agctttagta actgagtctg taaaaagtat aaatgcagaa aataaatttg
     2401 cactcactgg tacacctatg gaaaataatc tactagaatt atggtctata tttgatttta
     2461 taatgcctgg ctatctatat agtaaagcaa agtttcagga attgtttata aataaagaag
     2521 ataatgttaa aaacctgaaa aaattgataa aaccttttat tcttagaaga agtaaaaaac
     2581 aagttatgaa agaacttcca gataagattg aaaaaaactt ttttgttgaa ttgaataaag
     2641 agcagaaaaa gatatatagt gtatattcaa aagatataca agataaaatg aaggataaaa
     2701 atttaaagaa agataaaata gttatatttt cttatttaac taaactaaga caactttgtt
     2761 tagacccaag tatagtagta aaagattaca ataaaaaaag ttcaaaaatt gaaacatgtt
     2821 tagaaatact tagggatagt ataaacgaaa accacaaaat attattgttt tcacaattta
     2881 cttctgtgct taagaacata agtaaggaac ttgataagta taaaattaaa taccattata
     2941 tagatggtaa aactaatgcc aaagaaagac ttgaacttgt agatgagttc aataatagta
     3001 tggataaaaa agttttttta atatctttaa aagcaggcgg aactggttta aatcttacat
     3061 ctgctgacat ggtaattcac tttgacccat ggtggaatcc atcagtagaa aatcaagcta
     3121 gtgatagagc tcatagattt ggtcaaaaaa attcagtaca agttataaaa ttgattgcta
     3181 aagggactat agaagaaaaa ataataaaat tacaagaaag taaaaaagag cttataaatc
     3241 aatttataaa tggagaactt tctaatgaag gtgtattaaa aagtctatct gatgaggaga
     3301 taataaactt atttaactaa attatttaat cctacagctt aactatataa atttgttcta
     3361 acatttatat agttaagcta aaatatctga ataaataaaa tttaatattg ttatttaatc
     3421 ccaactttac aatctaatga tatctctttc tatacatatt gcatatacaa atgtaataac
     3481 ttgactatta gaatattttg ttttaacaat actatttcat cataataatg aagtatatgt
     3541 acactcactt ttgtaagttt tgcaatccat tttacagttt tcattattgt aaattttggt
     3601 taaattattc ctctaaaaat ttctttgcta catttgaaaa atctaatcca ttcgataaaa
     3661 ttgcaggatg accaccttta tcaaacaaat acattttacc atttttaatc ttttcaagaa
     3721 gtaatgaaaa agtattttca aaaaaatcac tagatattag agatacaaac tcatcttctc
     3781 tacttcctgt taaaagcacc tctggttgca tagtttctag aggcttatga aaaaactttc
     3841 ctatagtttt ataatgttca aaaatagctt ctgtatcatt atctacaact ttttcccagt
     3901 tttctccttg attataaata taaaaagctt ttgcaccatc atcttgctta gatgcttcac
     3961 gctctatttt cacattttgt acaaatgact ctagaggaac ttctccttca aaactatctg
     4021 caattacctt attcactaaa tcaggtctct caagtgcaac atttaatgca acaagagcgc
     4081 ctccactact accaattatg tttacttttt tatagttcat tgcttctaaa aaacatatca
     4141 cttgcatagc ctcatcaaac cataagtcag cagaaaattt atcgaccatt tgagattttc
     4201 catgacctac aaaatcaatt aatattactt tatattcatc tttataaaaa tcgattatat
     4261 cattaaacat cttggaagag gctgtatttc catgtaaaaa aagtaatggt gtaccatccc
     4321 caatttcatt atagtagcac tgtttgtcgt tatagttaaa atatgccata tatttccccc
     4381 ctttaatatc cctataggta tatatttatt acttatattt aaaatgacta ttcccttagc
     4441 cacttaaaaa tagtataact tagaatatag ataaaatcaa cattccttgc taatttatct
     4501 attgtttgtc taatttatta taatttaact tctgtaaaat ttcttttttg tacgaaaata
     4561 ttccttccat ttacaacaat cattcaaatg aagtataaca tgacgagtag gaggcataag
     4621 caaaattcca gcaatatctt tgttccccca aaagtccaac aaccaaatag aatcttcatg
     4681 ctttattact cctccagtca ccaaaatcct ttctaagtca attggagaga cctttcgttt
     4741 catatcttca gctttcaaat ccttaacaac ttctattgtg ttttttgcaa cttcatttcg
     4801 ataacaaagc aattcatcaa tattgactaa attatcgtat atctcttttc actttaacca
     4861 tttttttctt tataggtatc tccttatatt tttttaatat caaatctccc ttataattta
     4921 atatatctac atatgaacat aaatcttgaa cttcaattac acctatatca tcattagtta
     4981 taccttttat attactaaat gcaccaacta tatctaaaac tcttattttc ttcttcttac
     5041 cagcatttaa atataattta acaacttcac tttctatatt tagttttata tctttacttt
     5101 tgtaagaatt tttatcaact ttttttctct ttgatacttc ttttagtact ccttttgaat
     5161 attcttcaaa caaaactttg ccatgtgtaa ttctactttg ttctatatct tttaattcat
     5221 ttattttata tcctatatac gtttctatat tctctatata tttttcatcc ttttcagtta
     5281 ctatagtaat tgcctttcca ctatttcctt ttctcccagt tctacctatt ctatgtatat
     5341 agttttcttt atcttgtggc acatcatagt ttattactaa agaaatatca tcaatatgta
     5401 ttcctctgga cgctacatca ctactaacta atatattaaa tttttggttt ttaaaatcct
     5461 taattacaaa aattcttctt tcttgtgata aatctgcatg taactctctt attaaaaaac
     5521 cttctttact catcttatta tataatttag atactttctc tttggtatta caaaatatta
     5581 tcacactctt tggattttcg ctgtatatta attcttttaa aaggatgtac ttctctcttc
     5641 catttgcctt gattattttg tcatctattt gtcttgtttt ttgattagta tcgctctcat
     5701 tttcttctat atttataaca ctgtaatcta gcatatattt ttcacatata tattttatct
     5761 cttcatctat tgtagctgaa aaaagactta ctattttctc cttaggtaaa tttaataata
     5821 ttttttccat ttgttcaaca aaacctttat taaacatttt atctgcctca tctattacta
     5881 aatactttac attttccaac ttaatagaac ctctatttat atggtcaagt attctgcctg
     5941 gagtagctac aacaatatga accctttgtt tcaattcagc tatttggtct tttatagatt
     6001 gtttgccaaa tatagcacta catctcacct tctttagtct ccctatatct gatatctcat
     6061 cttttacttg caaggctaat tctcttgttg gaaccactat aagagcttga acattattgt
     6121 aatctacatt tatattctcg cataaaggta ttgcaaaact tgctgtcttt ccacttcctg
     6181 tttttgacct aactactaag ttttgacctt ttaataattt tggaataact tctctttgta
     6241 ctctagaagg tatattatat cctaaactct tcaatgactt aagtatcttt tcatttaact
     6301 taaatttttc aaatgtatat tt
//
LOCUS       DAEOSP010000193         6247 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.197, whole genome shotgun sequence.
ACCESSION   DAEOSP010000193 DAEOSP010000000
VERSION     DAEOSP010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.197"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            293..451
                     /locus_tag="KRH54_003045"
     CDS             293..451
                     /locus_tag="KRH54_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580680.1"
                     /translation="MKIFSFLLFVIGICLCIAGFIGHYPVFVGTTLSFLAIAMIAVRQ
                     VKTKKKMK"
     gene            complement(738..1442)
                     /locus_tag="KRH54_003046"
     CDS             complement(738..1442)
                     /locus_tag="KRH54_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8580681.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(1433..3040)
                     /locus_tag="KRH54_003047"
     CDS             complement(1433..3040)
                     /locus_tag="KRH54_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBG8580682.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(3294..4472)
                     /locus_tag="KRH54_003048"
     CDS             complement(3294..4472)
                     /locus_tag="KRH54_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580683.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(4698..5192)
                     /locus_tag="KRH54_003049"
     CDS             complement(4698..5192)
                     /locus_tag="KRH54_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG8580684.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(5193..5954)
                     /locus_tag="KRH54_003050"
     CDS             complement(5193..5954)
                     /locus_tag="KRH54_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBG8580685.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
BASE COUNT         2089 a         1166 c          680 g         2312 t
ORIGIN      
        1 aaaaaatgtt aaattgatat tatccatata taaattgata ttatccatat agatgctata
       61 tttaacaatt ctacttactt cccttacact tattagcata tagttattaa gagtttgttg
      121 caaaacaaca cttagcacac tttaaaaagt gtggtctaat aagtaatcaa cgaagaagac
      181 ataataaagc ataaaatatg atatactttt ccacgccatc aaaactgagg attttattat
      241 taatatatgc taaaatatgc ttaataaatt aaattttaat gggagaatag ttatgaaaat
      301 atttagtttc ttgttgtttg tgataggaat atgtttatgt attgctggat ttataggaca
      361 ttacccagta tttgttggta caacattgag ttttcttgca attgcaatga tagctgtaag
      421 acaagtcaaa actaagaaaa aaatgaaata ggcaaatcct aactttacaa gttaatatta
      481 cataagtaat tagtctaaaa ttggatactt tttctattac agaaatacat catccacttt
      541 gtattttctt aaaaaaataa aatagctatg taaattcaag attagataga ttaactctct
      601 atccatttca ttgtaaaata tacatagcta tttgtattcg atttaaaata cttagatttt
      661 cttacataac aaataatttc tatctgatta ttactcatat tcatttagta atcactcagc
      721 aactaatcat ttagtatcta ttcatccgcc atacggtaac ctaccccaac ctcagtcaaa
      781 atatattctg gctgagcagg attcttttca attttccttc ttatacttgc cataaaaact
      841 ctaagcgact gattttcact ttcatagtaa tttccccaaa ctttatctat tataaactta
      901 tgggttaaga ctcttcctga atacttagac atcaactcaa taatcttata ttcgattgga
      961 gttaaatgta tttcttcacc attaattgac acaatatgat tttcataatc tatctttagg
     1021 tttttaactt caaaagactt tggagcatca ttatttatat tatcctgttg agctttattt
     1081 ctaagagcca cacgtactct agccaaaagc tctgttacac taaaaggttt agttaaataa
     1141 tcgtctgctc ctccatctaa agcttccacc ttatccctct catgctctct cgctgaaaca
     1201 acaattattt tattagtctt tgcacatgcc ctaactcgtc ctattacctc aatcccatca
     1261 atatctggta gtcctaaatc caaaattatc aaatctgggt caagagaatg tataagtgat
     1321 atagctgttc ctccattatc tgtctctaca cacctataat tttgagtttc aagagatact
     1381 tttataaatt tacaaattgg cttatcatct tctacaatta gaactaatgg ctttatttcc
     1441 acttgtcttt cccctcctca tcatacatag gtatgctaaa tctaaatgta gctcctccag
     1501 aaggattatt aaaggcgaca atttctccac catgagcttc tactatagat ttacatatct
     1561 ccaatccaag accaatacct cttctttcaa gatttctaga cttattctta gtataaaacc
     1621 tattaaaaat attcccgata tcttcatttg gtattccttt tccattgtct gcaacttcaa
     1681 atagtacttg atttgactct ttttttacag ttagttctat cttagaattt ttaggtgtat
     1741 gccttatagc attatcaatt agatttacta acacttgttc tattaataat acatccacat
     1801 gaacgattat tatctcatcc ggaatatttg tttttatgtc tctactattg gcaaactttt
     1861 tgactcttaa tatagattca gctattattt catcaacaac ctctggtcgt ttttcaatat
     1921 ccaattttcc ttcatctatt ctggtcatac taagtatatt ctcaacagaa tgagttagcc
     1981 aactcgtatc ttcatatata tttttcaata attctactct agtttcttta tctatcacat
     2041 catcattttc aagtattgtt gaagatgaac ccaatatact tgtaagtgga gttcttaaat
     2101 catgagaaat tgacctaagc aagtttcctc ttaacctctc tctctctgcc tcaagatttg
     2161 cttttttatt tttctcaaat aaattttctc tatcaattgc caacgcaacc tgtgttgaaa
     2221 ctgcttctaa tagtatcttt tcattttctg taataatatc tatcttaaaa cacgcaacac
     2281 ctataacacc aagaacattt gattcttgac cttttatagg gaaataatat gcactgctgt
     2341 ttttacaata ttttgtccca attccaactg ggttacctgt cctaaaagct tcttttatag
     2401 cttccttttc taatactgaa tggaatatat tctcacttcc atcattgtta caaatataaa
     2461 tacttggctt acctagatta ttattctcat ctgcaatagt cactattaca gttctattta
     2521 atatatttac taaattagac ccacaaaatt caataatttg atttttattt tttgctttaa
     2581 gcaacgtttt gttattctca tagagcaatc ttatcatttt ttctctctta tgagaaagtc
     2641 tcacttctct ttttatccta gaagttaaag tacttgttat aattgctgcc attagcataa
     2701 tacaaaaggt cataggataa tcttttctat aagctaaaaa agtatataat ggatgcgtaa
     2761 agaaaaaatt aaatgatagc actcctatta ctgaagaaag tattccaaaa aaatatccat
     2821 aggttatact tgtagcaaat agtacaccta atatgtatac tagtataata ttagattcat
     2881 taaaacctac cttttcaaaa actactgata ttccagtagc aattgtcata accaatatag
     2941 tttttatgaa gtccttaata attatctttt tactgagata gccatcttca tcataatcat
     3001 actcttttaa gtcttcatgt tgtagtcgtt cctttttcat ttactacaat tcctccttat
     3061 tgttactgtt taaattggct ctattttata taatacaatt ttatcataat aaattcaatc
     3121 aatacattta attaatgtac ttaaattact aaaaggtgct gtcttaaaat gaaaataaat
     3181 ctctatttca aaacagcacc tatatattta aattactaat ttagttttta catacttaaa
     3241 tcaatagttt tattcaattt cataaattat aatacttcaa ttataaaaca tttctatcca
     3301 acaagcataa atataaagtg ggcacatata cctgcacata aaccacctat agtatgcgaa
     3361 agtatcgctt taccagccaa tggtcttgca tctaaagcat ccatcatacc tatgtgtgta
     3421 cttaaatatc cactccaaca catacccatt gctgtgaata ctgctatatc attaggagtt
     3481 atagcgcctg attttataaa ctctggtact aaagatattg ctgctccaac agctcctaat
     3541 gcagttacag ggaatgctat tgcctctggt gatgtgaatc caaataatgg ttctattatg
     3601 aagctaatct tatcaccaat agcaggtaat aattttatac cttcatatgc agctcctgtg
     3661 taaacagctt gacctgtagc agggtctgta gatggtccaa aagttaataa cataactaat
     3721 gtacaaacta ctaaaactcc tggtattata gccataccca tttcaacacc taacttacca
     3781 ccttcaagta tagcatccaa tgttctttgg aatacattac catctcttat ttctctaaac
     3841 tcttcaagtt ttgcaccttt atcatttatt tgcttttcag tatcattttt agggtcataa
     3901 ttataatact ttttagtaaa agtcaacata attctaacac taataatact acctattatt
     3961 gccgcaacat tacctattat agctggtagt acatattctt taccttgagc tatcataaaa
     4021 gttgttacaa taagtcccat accaaaggct gttcctaaat tacataaagc aggtacttga
     4081 tacttcttaa aatattgtgt aaatgttttg tctttagcaa atggtataat agctggatta
     4141 tcagataaat atgttgcaac tactcctgct atactagcac caggtaagcc ccatataggt
     4201 ctcataagac ccttaaatat cttattaact aaagatatta ctccaaactc tgaaagcaat
     4261 gcactaagtg cacctgcaag aactgccata gccattatta aaaatactgt gtctaataaa
     4321 agcgcatgtg ctgtactcat tattacttta aacatcattc ctgcacccat gatgcttcct
     4381 acatatccaa atcctaccgc caatagtaca ataaatacga aggtttccat acttactgcc
     4441 ttgactcttc tcttatccga cagcttttcc atcttgaaat cttccccctt ttaattttaa
     4501 attaaggttc ctaacattgg tacaaatttt ttggtacaaa ctgagttcta aaacttacac
     4561 tcgtaatgca agttgtcagt tttttgaaat aaaaaaaaat tacgtttata gaataagaat
     4621 atcctcttta atttggatac tcttattcta ttttatgtta ttatattatc atccttttgt
     4681 ttaaaagtca attttttcta tttttgttgt ttaattttat ttatactctt tttatatttt
     4741 tcattttctt ttatctttgt aagtactcca tataagttga cttttctaaa tacagtagtt
     4801 ataaatacca atcctaatgc aattgaactt gcacacgata atgtactcac cccatattct
     4861 acagctttta tataattatt ggtcaaaaca gaatacatag tattatatat tccataacct
     4921 ggtactaata ttattaaaca gcaaacactt aatgtagttg ctggagtcat ataaattctt
     4981 gcacataact cacaatatat cgcaaaacaa actgtagcta taaaaaaaca taagttttct
     5041 gttactccca tatttaaaag aaatttatac gaaaaccaag ctatacttcc acctattcct
     5101 gctagtatta agttttttcc tttcatatta aatagcactc caaagcttaa ggcagtaaaa
     5161 aaagctgcta ttacttctat aaacaaatgc acttatatcc cccctaactt gaagtacaat
     5221 ccaagtacca agaacattcc tactgctata gatacaccta caaaaaatac ttctgctaat
     5281 tttgcaagac cagaaatcaa ctgtccatct aataaatctc ttaatgcatt agtcaaagca
     5341 agcccgggaa ctaagagcat tattgctcct gctattaaat ggtctaacgt ctttatgaat
     5401 ccgagcttta aaattaaaat tgaacaagaa gctataataa ctgctccaat tgcatttaca
     5461 aaaaagtcat taagggaaga tttacttaaa tacataataa caaacttagt cagaatccca
     5521 acagtaaatg cagctataaa ttcttccaca tttcctccaa ataatatcga aaaagttgct
     5581 gctgcaatac ctgcaaaaaa tattgttatt aaatccgagt aatagttatc ttcacttatt
     5641 tcatctaaaa ttttctcaca taattcaata tctaaattat ttatacttat tgctcttgat
     5701 aatgagttaa cttcatgaac caagtttagg tctactcttc ttgagcttat tcttttaatt
     5761 gctgaatgta atttcccatc tacaaataca gatgccatta cagctgttgg agttgcaaat
     5821 acatctacgt gatctattcc aaaagattga catattctag ctatcgtttc ttcaactcta
     5881 taggtttctc ctccactttg caacatagct ttacctgcat ttgaggaaaa gtttaaaatt
     5941 ctatctatat ccacaaaaca tatctcccat tcttttatag tattattcat attttaatac
     6001 aagatattat ttatttaaac tattttaaat aattcatatc caatatttac attttatgaa
     6061 aaaacttgtt ttttatgctt gtcaaaatca aattttaagc ttgtttacac cataaaatgg
     6121 ctgaattaat atttgacaaa cactttcaac catttatata accttctaaa ttgtataact
     6181 ttttactaca aataaaatta tatctctttt aattctttct cttgagtatt tagcttatag
     6241 taacttc
//
LOCUS       DAEOSP010000194         6229 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.198, whole genome shotgun sequence.
ACCESSION   DAEOSP010000194 DAEOSP010000000
VERSION     DAEOSP010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.198"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(206..589)
                     /locus_tag="KRH54_003051"
                     /pseudo
     CDS             complement(206..589)
                     /locus_tag="KRH54_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      830..927
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            982..1158
                     /locus_tag="KRH54_003052"
     CDS             982..1158
                     /locus_tag="KRH54_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580686.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGESVVE
                     SCLHQVMVLVSKYN"
     gene            1384..1584
                     /locus_tag="KRH54_003053"
     CDS             1384..1584
                     /locus_tag="KRH54_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG8580687.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     repeat_region   1949..2116
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1949..1976
                     /rpt_unit_seq="tttacataccacatagttgatataaaac"
     gene            2587..3432
                     /locus_tag="KRH54_003054"
     CDS             2587..3432
                     /locus_tag="KRH54_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580688.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKSLYFNKDNEDPKYNLNTI
                     KKHLN"
     gene            complement(3656..3820)
                     /locus_tag="KRH54_003055"
     CDS             complement(3656..3820)
                     /locus_tag="KRH54_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580689.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
     gene            complement(4362..4694)
                     /locus_tag="KRH54_003056"
     CDS             complement(4362..4694)
                     /locus_tag="KRH54_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBG8580690.1"
                     /translation="MNTREMKEYRVFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(4938..5579)
                     /locus_tag="KRH54_003057"
     CDS             complement(4938..5579)
                     /locus_tag="KRH54_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBG8580691.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            complement(5560..5853)
                     /locus_tag="KRH54_003058"
     CDS             complement(5560..5853)
                     /locus_tag="KRH54_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBG8580692.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
BASE COUNT         2348 a          842 c          659 g         2380 t
ORIGIN      
        1 ttatctatag tgttacttat attttatata tatggctatt tttcaacatt ttacaatata
       61 tataacattt atttttttaa cttttgtcag taaaatgtca gcaaaacact ttatccacaa
      121 tttttatata agttttccac aacttatccc cagtattatc cacataaaaa aggtagctct
      181 tatattatta gctacctttt atttattaat cttcatattt ccagcttttt atccaacttc
      241 ctatttctat tattttttct tcagttattt cttctccatc atgtaatttt gaaattaatt
      301 ctgataaagg attgttatgt aaacctctaa aaactctttt ttcctctatt ttgatatatt
      361 ctctctcttt tattaatact gtatagtgta catgcctccc aattctctat gatattaaaa
      421 aacctttttt ggttaatcta gctaaaagta taattgtagt tgtatgcttc caaccatatg
      481 ttttttctat ttcatctgca acatcttttg atattacagt tttataacca gtattccata
      541 tgcatttcat aatttttaat tctgtatctg gtaatttttt atttagcata atatattcct
      601 tctttcaatt tatgtcactt cttcttatgt cacttcttca aatttatcta gcttaaatta
      661 taataggagc tttttagaac aaaaactata tttaattttt accaggtact ctacgtgttt
      721 atcctgtttt taagtgtaaa ataatgttca tatctataaa tttatttaac tttcaaacaa
      781 tcctgttgaa taataactaa ttctattgta ttattttagt tgtagaataa aactaaatcg
      841 gcaaaactag agaaatttag tgacgcaaag ctatagggac taagacttat ataaatatct
      901 tatgagttat gtcagccagt tgccaaagag atagtgttct ttttgttttt atgaaagttt
      961 tttaggggga taataattta tatgtttgga aataaaatgg ataaatgtac acatgtattg
     1021 actgcttata ttagcagttc gtatgattat tgtaattttt tagatacaca attagatgat
     1081 tttatattag agtacggaga aagtgtagta gagtcttgtt tacaccaagt gatggtattg
     1141 gtaagtaagt ataattagat agacattgat ttctagagaa ccctttttag ttttatctac
     1201 atttatatta atatatagca atgtaaaacg tttaaatata tttttaatat taagaataac
     1261 taaaatgtta actaaaaaat ataattaagt agacatttct attaatctat gatacaatag
     1321 tatttgtggt aatacacaat taaatcaagt atatcttgat ataggttaat aggagtattt
     1381 ttaatgaata atggaatagt gaaatggttt aataatgaaa aaggattcgg ttttatatca
     1441 atggaaggta gagatgatgt gtttgctcat ttctcagcta tacaaacttc aggatttaaa
     1501 tcattagaag aaggtcaaca agtaagcttt gatatagtta aaggtgctag aggtcctcaa
     1561 gcagaaaata taactatatt ataatatata gatttttcgg gaaagactct ttttagagtc
     1621 tttttttatt gttaatatat gtaagataat cattggtgaa gtaaataagg tatattgagt
     1681 aatgttcttg atagtttata tgctaatgaa tttcaaaaaa ataagcactc ctataaaaag
     1741 agtgctctta atgtaaatat aaaccttcat tggtccagcc taataccccg tattctccaa
     1801 gttctaactc ttttttatat acattatcac tttcagccat atctaacaca atcatttctt
     1861 ctttagaatc aatttgtaaa atatgaggac tgtgtgttgt gacaattatt tgtgtttttg
     1921 ggagaattgt ttttaatgta ataaaaattt tacataccac atagttgata taaaacgtaa
     1981 atacaccatc tataaagggc aggtgttaaa actctttaca taccacatag ttgatataaa
     2041 acttaatata cttaggtatt cctactggaa acttttctct ttacatacca catagttgat
     2101 ataaaacccc aaaataaact tagcatctcc aatgtttaca catatacaat gctcttaaaa
     2161 ttgcagtgaa cgcgcaatag tgcaattgat aatacttatt atatattctc aatgccttat
     2221 atttcaactg ctaaacctca atttgtcaca aatatcgctc actgcaaaat ttctatattt
     2281 ttattatatc ataacatata attaatattt tcaattaatt tttacattta attatgttta
     2341 aattctataa taatgcaact ttaatgtttc tttctggaac taatataaaa actatttcag
     2401 aaagattagg tcatgcagat ataaaaataa ctatgaatag ctactctcag tttttagaag
     2461 agatggacag agaagcattt gaaaatttga gcaaaatact atttattata ttttaaagaa
     2521 gatgacttat tcaatatttg atgctagaat ataattaata aaaatttaat tataggtgat
     2581 tgtattatga atattattaa aagcataact ttactcttaa cattattaat tgtgatatta
     2641 atgacaggct gttccccaag agaaagtgct agtgaagttg caaaaaaata tattgataag
     2701 atgaactctg gtgactatga aacagcatat agtttattaa gtaaagatag taaaaaaaaa
     2761 attagtatag aaaatcttga agaatttcaa aatattttag ttagtacaaa aagaataatt
     2821 agttataaaa taggtaaaga aaaaatcata aaaaaatata aacatgatgg aaaagaatat
     2881 aaaaatgttg ttaaattaga agaaactttt aatataaaaa accttcttta taaaaaagat
     2941 gattctctaa aagtaaatag atattttgta gttgaaaata acaaatacaa gctattattg
     3001 tatgaaagtt tcaaaaaaga atttggtatt aatagtatgg accttgctca acttaagctt
     3061 aatgattttg gactaaaata ctttaaagaa gttgacctta tactaaaaaa agctattgct
     3121 attaatccta ctgattcaaa gctttattat gcccaagcat gtaattatat gaattttgat
     3181 ggatttcctg gttattcaga tacaacatat aatgctttag attcaattaa taaatcttta
     3241 aagtatttag ataaaaatga ttctgactac aaaaaaaatg caagtaatat atataactta
     3301 aaaggagtta ttcttatgtt tcacaaaaga tttgatgaag cacaacaata tttaaataag
     3361 tccttatact ttaacaaaga taatgaagac ccaaaatata atctaaatac aataaaaaaa
     3421 catctaaact agatatttaa tttttgaatt tataaaatat ttttttatgt ataattcagc
     3481 actgaatcat aatctctact aaactaatta caagtttaaa ttataaagac ttatttctat
     3541 ataaatatta ctctataaca ataagtttct tatttaaatt ttaaaatatt atctaaaaat
     3601 cataaaaaag aaggtataaa gagctaccct caagctcaat ataccttata aatatctaaa
     3661 cttcattttt tactaatttt tctatatctt tgataatatg ttctttagat ttttttccag
     3721 ctatggcatc aaaaattgca acagcctttt tctctccaaa cttttctgct acatcattaa
     3781 agtctaaaaa tctaacactt tcttttggtt taccaaacat ataacacctc tcatacattt
     3841 ttatatattt attataactt ctatagactt atttttcacc aataaacgtt agacaaatta
     3901 gtccctttat ttttttaaac tagttttacc ttcaaaattc ttaacaaaaa cagtttattc
     3961 tactctataa ataaaaatag acaatttaat ttgtaggaaa ctgataatag aatacagtaa
     4021 ataaaacttt gtttaatcta tctgaaaaag ttatctatta aaactgctat gaatttataa
     4081 agaatattct agttcataaa aatacaaata tgtaaatata atcaccttaa tattcaattt
     4141 atttaaaata aaaaatgtat aaaagattta ataatgacaa tatactattc tcaatacaat
     4201 ctatgatgta aataatttaa aactactata ttatttaaaa atttatattt atatatgcaa
     4261 ttctaaaaac ttaataagaa gtgaacttag aaaataaagc ttaatttcaa agacttaaat
     4321 agactattat tattagtcta aataaactac gtctatcaaa attatatatt aaattcttct
     4381 aaaattttta ttgcaaatct tgcgtttgga tatgtaatag acccgccacc aattacacct
     4441 atttttatag cttctataat ttcatcttta cttgctccag aattaataca accctcaata
     4501 tgataacaaa tacactcatc acatcggctt aaaattgaaa tagctagtcc cattaattcc
     4561 ttattttttt tacttatagc cccttctgaa taagtcttat catcaagact tccaaatact
     4621 ctaaaaaact catctgtttc gcttaatttt ttattgtaaa ataccctata ttctttcatt
     4681 tctctagtat tcatcttcta cctcctatgt ttttcttata agaatatcac actgtactta
     4741 tattttcaat attaaataca aattttcatt ttatgttcat tagcctaatt tatattattt
     4801 tttgaaatct caataagtta tcatataagt aagatttttt cagaactaaa atagaaatct
     4861 tagtaatttc tttattacct atctcagttc tgaaatatat tattcttatc caaacattat
     4921 tatattgatt aattatatta gttattttta cctgaaattt tatacatcaa ataagaatat
     4981 ataaatggta gtattacaat tacaaagatt acaaagatat tataaaaagg aatattaata
     5041 aatgcattaa taattaataa tattccacca ataagccaaa tccatccagc taaatggtgt
     5101 gtcttattcc aattatcttc atcattaaga gtccatggca aaattattcc tactgagtaa
     5161 tttcgtttac atttaggaag atagtttcca aataaaataa ctattattcc taaaattaat
     5221 cctgtataaa aattagactc caaactaact cctgttgaat ataaaaccat cattatttgt
     5281 ataattactg aaagtatagc aactccccat tttgataaat tttgtataac atttgaaaaa
     5341 ttagctttct taggctcatt ctcaagtcta aaccatgtat acaaatggat acatagcatt
     5401 aacaatggta caccaaaagc tgctataacc tttggagcat aattatctgg atttccataa
     5461 ttatcaaaat gaattgcaac ttcatttggt aatctattat aaaaatatat tgatacaata
     5521 aatggtataa aacatattaa agtagataaa ataaattgtt tatttatcgc ttttttcatt
     5581 atctttttct cccttctcca taaattgtga aaaccaaagc ataacctctt cgaaaactga
     5641 aatatttatt tcgtagaaaa taaagttctt atactttgtt tcaaatatga gtcctgcctt
     5701 ttttagttga gatagatgat aagaaattgt agcattcgtc atattaaatt tttcactaat
     5761 ttctcctgct gacattttat tctccctcaa cataaccaat atttctcttc ttatagggtc
     5821 tgaaagagct ttgaatgttt ctggaaaccc caacaccatc acctcgctat ttagaatttc
     5881 atctaaatag aatataacat aacatccatt ttatttcaat agctatttag aaatttttct
     5941 aaatagctat ttctaaatag ttattgaaaa ttttcatatt aagattctca atttacttaa
     6001 gtcgctttta tttaatctta ctttatctaa tttttttaac ttaacaaaag tgtattgcac
     6061 cataattgtt aaagcaaaaa cctaatgact gagatactta tatatataac aatactttta
     6121 attttaaatt aaaagtagct aaacccaatt taaaaagtag ttaagtccta tttaaataga
     6181 aaagaatgaa acgagaaata tactgtataa aagtacataa aaacatcta
//
LOCUS       DAEOSP010000195         6224 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.199, whole genome shotgun sequence.
ACCESSION   DAEOSP010000195 DAEOSP010000000
VERSION     DAEOSP010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.199"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            107..652
                     /locus_tag="KRH54_003059"
     CDS             107..652
                     /locus_tag="KRH54_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBG8580693.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            665..1678
                     /locus_tag="KRH54_003060"
     CDS             665..1678
                     /locus_tag="KRH54_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBG8580694.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            1900..3873
                     /locus_tag="KRH54_003061"
     CDS             1900..3873
                     /locus_tag="KRH54_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBG8580695.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDLSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNCILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDEKFRKNIVNREEKSPTVFD
                     KGLLFPHSINEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            3942..4301
                     /locus_tag="KRH54_003062"
     CDS             3942..4301
                     /locus_tag="KRH54_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580696.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            4336..4692
                     /locus_tag="KRH54_003063"
     CDS             4336..4692
                     /locus_tag="KRH54_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBG8580697.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            4729..6021
                     /locus_tag="KRH54_003064"
     CDS             4729..6021
                     /locus_tag="KRH54_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBG8580698.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
BASE COUNT         2459 a          584 c         1182 g         1999 t
ORIGIN      
        1 catttacaat attagcatat agtacagaaa aaagatgtaa tttgtaaaat ttatggtaag
       61 cagtaaaatt aaaaatttaa agatattata gggaggttat aattttatgg aaaacgttat
      121 tacaggtcta agtaaaggtg cagagtggtt tatcggtttg tttcagaaag ggggagaaca
      181 atttataggt cttgtttcag gtactcttcc aacgctaatt gtattaatgg tagctattaa
      241 ctctttgatt aagataattg gtgaggaaag agttaataat tgggcttcta agctaggaaa
      301 gaactctttt acaagatata ttctacttcc attagtatca gtatttttct tgggaaatcc
      361 tatgtgctat acatttggta gatttttaaa agaagagcac aaagcaggat tttatgatgc
      421 agcagtttca tttgtacatc caattacagg tctttttcca catgcaaatg caggagaact
      481 ttttgtatgg ttgggaatat cagcaggttt gactacttta ggtaaagaaa ctactacact
      541 ggcattgtgg tattttatag ttggtctaat tgtaattttt ataagaggta tagtaacaga
      601 aaaaatgtat gcttttctaa caagaaaaaa tagagcaaca aataaggctt aggaggtaga
      661 taaaatggag gagaataaaa tattaaaaat agaaaaggga acttcaggct ggggaggtcc
      721 tttgtatata aaaaaagaag ggaatagaaa taagatacta tcaatgactg ctggcggtat
      781 ccatgaagta acattaaaaa taaaagaact actgggttgt gaaattgtag atggatttaa
      841 aactggagtt tctgatgaag aagtagcagt agttattatt gattgtggtg gaactgctag
      901 atgtggagtt tatccaaaga aaaaaatacc tacaataaat gtaaatccag ttggaaaaac
      961 aggtccttta gcaaaattta taacagaaga atattatgta tcagatgtta atcctaactg
     1021 tataagtgtt gtagatgggg aagatatgcc acaaaaatct caagaaaata aaagcgaaaa
     1081 taagagttct attagaaagc ctgataatta tgatgaggtt aagtcaaaag cacaaggtga
     1141 atatgctaaa aagaacataa tactttctat tggtcaaggg gcagggcaag tagtatcaaa
     1201 attttatgat gcaggtcgag atactatcca aatggtaatg aataacgtaa taccatttat
     1261 ggcatttgta agtatgctta tgggaattat tttagcatct ggactgggag actggattgc
     1321 aagagttata tctccacttg caggtaatat tggtggatta ttgattatat cagtaatttg
     1381 cacacttcca ttcttatcac caatactagg acctggtgca gttatagctc aagtagtagg
     1441 gactcttgta ggtacacaaa ttggtcttgg tgcaatacca gcatatttag cacttccagc
     1501 tttatttgca ataaatgggc aagctggatg tgattttgta ccagtaggat taagtttggg
     1561 agaagcagaa ccagaaactg tagagtatgg tgttccagct ttattttact ctcgtttaat
     1621 aactggtcca atagctgtta ttattgcata tggagttgca gtatttgcat tgagataata
     1681 tttaatgtca atataaggct tggaattaat taataaaatt gtaaatataa atttaagcta
     1741 gtcaagattt tttgttaatg aaagactagc ttttatacta ttaattagct gtaattatcg
     1801 agttagatta atactaaatt ttcctttatt aaagtgaaat tttatgtatt aatttggttt
     1861 caaatttgta ctagaatgaa atcaaggagg agatacaaaa tgagtgtaac tgagaaaatt
     1921 ccttctattg accgcaagat atttaagata ttgattatgt gtagtaaaaa agagtttgtg
     1981 agtattaact caatagcaaa tgaattgaat gtcacaacta gaagtgtaag gacgtatata
     2041 aaacaattga ataaagattt aggtaatgat atagcagtaa tcaaatatat aaagggtcaa
     2101 ggctataaac tagaaataaa agatgagcag atactcaata agattattga tatgaataga
     2161 aaaaatgtat tttcattaaa ttcaaaagaa gatagagtag agtttatact aaattacttg
     2221 atagaactag atggatttat tacacttgat agtttagctg atgagatgtg tgtaggaaga
     2281 actactttag taaatgattt ccagtatgta gaaaaagttt tggcttcata taatttaaac
     2341 ttgattaaaa aacagaatac aggtatgaaa ttaaatggaa atgaacttga tattagactg
     2401 tttatattaa atcagttgta taaaaactca agaaaagatt taagtaattc aaagtatttt
     2461 aaaggaatca aaaaagaaga aatcataaat ttagaagaaa aactattgac tttatttaaa
     2521 aaaaataatt tttatgttac agatgaaatg ttaagagaag ttattaacta catagtagtt
     2581 ttagtctata gagtaaaaga agaaaagaaa gtaaaagatt atgatgtaaa gtttgattta
     2641 cttaaatcat atgatgaata ctttattgct agggaaataa aacatataat atcaggaatg
     2701 tttgaatgta ctttaaatga tgaagaaatt atttatttaa ctataccgtt agttagtgga
     2761 aatgcaccag catcagaatg tgctttaaat agtagtagaa ttagtaaaaa cattgatgag
     2821 ttgatggaaa atatatttaa tcaaatatat gtcgatatgg gtatttttat aaatgaggat
     2881 gaattaagag taggtctagg atatcatctt agttttactt taaacaggct attatttaat
     2941 ataaagttaa aaaatgtttt gttagaagag attaagcaaa attgtatact tccatttaaa
     3001 ttagcacaaa tagcaggaaa agtaatagag aaaaagtata acttagaagt ttcagaagat
     3061 gagattggat atatagcaat ccatttcagt ggatatttag aaagaaatag cagtagattc
     3121 tattcaataa aaaaaatagc aattatatgt agtacaggtc taggaacagc aaaactattg
     3181 aaaataagag tagaaaaact gataggaaat aatcctaaaa ttgatactct ttcaagtttt
     3241 aatttgagaa atattaactt agatgaatac gatatagttt ttacgactat tgatttagac
     3301 acatcaaata taaatacaat agtattaaaa ataaatacaa tatttgatga aaacaaacta
     3361 agagaacaat taaaaacagt attgtgccta agagaaggga atattgacac tactaattct
     3421 acaaatttac taataaataa tttgttagat gaagataaat ttatgatatt aaatgaaaag
     3481 actatacttg cctcattaga aaaaatgatg gaccatctta tggatttggg atgtattgat
     3541 gagaagttta gaaaaaatat agtcaataga gaagaaaaat ctcccacagt atttgataaa
     3601 ggtttattat ttcctcattc tataaatgaa aaatcagata agtttttaat ggcagttgga
     3661 attttagaag aaccaataga atatgcaaat agaagtataa aaataattct tatgacaatg
     3721 tttccatgtg agaataagat agactcagac ttattagtaa aaatttatga agaagtttta
     3781 aagataggcc aagatgtaaa attaacgaat aagataagta aatgtagaac ttttttagaa
     3841 tttaaaaagg tgttacttaa aaaccttata taactattgt acagcattta tagttgttat
     3901 acagatttaa aaataatgtg atttttggaa ggtgtataaa tatgaaatta ttagtaattt
     3961 tatttgttgc ttatatttta aatactatat ttataatgaa gcagaataaa ttgtatttta
     4021 aaactttaaa tagagctaaa aaaaagggtg atatagtgtc tacaggcaaa aagaaaagct
     4081 atttttctaa aggaagtata gccattatat cttcagattc agatgggttt ataaagtttg
     4141 gagaaatttt aaaagggcgt acagtaatgg ctaaatttaa agagattgaa gaaatagaag
     4201 gattagatat atatagtgca gagcaaaaat ttaaagatga agagagtatt gtacaagcta
     4261 taaaatttat aaaagaaaaa ataaatgttt cttttaatta actcaatata aatataaatt
     4321 atcaggagga atgtcatgaa atatgaagtt aagataagtg ggataggcag tttagttaaa
     4381 gagttaatgg atgaaagcaa ttgtcttatt atatatgatg aaaccattaa tgatgacgac
     4441 ttaaaggata tatctgtaat tcattcaata gcaacattaa aaagtgatgt tgaaatagga
     4501 gatacactta ctatagggaa tagagattat tgtatagttt cagttggtga tattgctcag
     4561 aagactctta gagaaattgg gcattgtaca atcaaatttg atggaaaatg tgaagtaaat
     4621 ttaccaggag aaatacatgt ggaaatagga aatccagata taactattgg agatttaata
     4681 actatttctt aatttgaaaa taaaattatt ataacaggag gaattggtat gttagattta
     4741 aacagaaaac ttgcaaaatt ggaagaggaa ggtaaaaaaa taaaagtagc tcttatagga
     4801 gctgggcata tgggaaatgg aatggtaagc caaatggcaa atatgaaagg tattgaagct
     4861 tctattgttg tagatataaa tttggagttg gctcataaag cttttactga tgcaggaata
     4921 gatgaagaaa ttatagataa tgtaacaaat gaaaaggatg cagaaataaa attacaagaa
     4981 ggaaaagtat tgacatgtaa tgactttctt gtggcttgta aaactaagtc aatagatgta
     5041 gttatagatg caactggggg tattgcaatt ggtgcagaga tagcattaaa ctctatttta
     5101 aataaaaaac atatagttat gttaaatgct gaaactgatt gtgttgttgg accaatactt
     5161 aaaaaattag cagatgatgc tggagtaata tttacaggtt ctgcaggaga tgagccaggt
     5221 gcagtaatgg aattatttga ttttgcagat gcaatggggt ttgaagttag agtgataggt
     5281 aaaggtaaaa ataataaatt agatttagat tgtaatcctg atacagttag agaagaagca
     5341 gaaagaaaag gagcttcacc tcatatgata gcttctttta aagaaggaac taaaacaatg
     5401 gtagaaatgg ctttaatgtg taatgcaact ggatttgtgc ctgatgtaag aggtggtcat
     5461 ggtattgaag ctacagttaa tgaagtacct aagaaatatg cattaaaaag cgaaggtgga
     5521 gtattagata attatggtgt agttgatttt gtaaatggta tagcacctgg agttttcgta
     5581 gtagttgccc ataaattaaa agcagttaat gatgaactaa aatatttaag tatgggtgat
     5641 ggacctaatt acatattata tagaccatat catttatgta gtttagagac tcctttatca
     5701 gcagctatgg cagttttaga ccataaggct acaatagtac ctaaagctgg actagtagca
     5761 gaagttatga ctatagctaa gaaagatttg aaaaaaggtg agtttatgga tggctatgga
     5821 gcatatacat gctatggtac tatagaaaaa tacgatgtag caaaggctat gaatgctgta
     5881 cctattggtt tgataagtaa aaagactaaa gttgtaaaag atattaaaaa aggtgaagtt
     5941 ataacttacg atatggtaga gatagaaaaa gatactactt tatatcattt aagacaattg
     6001 caagaaaaaa tatttgggta agatttgatg gtaatataaa tagatattag taaaaagggt
     6061 tgatggttat ttttaaatca acccttttaa atttttatat attcttgtta ggatactttc
     6121 tttaaaagta tattataggt tatttgtagt cgtaaaaatt attttgaatt agttctgcaa
     6181 ttttatattc acattgaaac aatattatta aaaattttaa tata
//
LOCUS       DAEOSP010000196         6163 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.200, whole genome shotgun sequence.
ACCESSION   DAEOSP010000196 DAEOSP010000000
VERSION     DAEOSP010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.200"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..392)
                     /locus_tag="KRH54_003065"
     CDS             complement(<1..392)
                     /locus_tag="KRH54_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580699.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTK"
     gene            complement(866..1750)
                     /locus_tag="KRH54_003066"
     CDS             complement(866..1750)
                     /locus_tag="KRH54_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG8580700.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2041..2517)
                     /locus_tag="KRH54_003067"
     CDS             complement(2041..2517)
                     /locus_tag="KRH54_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBG8580701.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            2783..3958
                     /locus_tag="KRH54_003068"
     CDS             2783..3958
                     /locus_tag="KRH54_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBG8580702.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4039..4842)
                     /gene="proC"
                     /locus_tag="KRH54_003069"
     CDS             complement(4039..4842)
                     /gene="proC"
                     /locus_tag="KRH54_003069"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG8580703.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(4882..5466)
                     /locus_tag="KRH54_003070"
     CDS             complement(4882..5466)
                     /locus_tag="KRH54_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8580704.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2073 a         1026 c          667 g         2397 t
ORIGIN      
        1 attttagtaa attttacggt aagtaaaaac ctctctcctt caaatcctat agcaaatata
       61 cttacaagtt gctttgagaa aataactcct aatatagcta caactatgca tataccaact
      121 attatattta aaacattgtt taaaaacttt agtgcttgct tttcaccttg tttactactt
      181 atatcttgat acattggaat aaaagtagta actatagcag tacctattgc tgcaaatata
      241 atatttggta tattcatagc tgttaaataa ctctctgtat ataagcctgt accataaatt
      301 gatgataata ctaattctct acccattcca agaatcttag agattatagt aactatcatc
      361 aaataaaaag ttgctttagc taccttgctc attttatcac tccacttcta acatacctct
      421 ttttaatcaa gtaaaattta gctcattaga tataattcta tcaactaatt tgccttgctt
      481 agccctatca aaatataatt aacacctatt tcttaatata tcacaaacta cattattctt
      541 catatttttt tatttctcca ataagtttta catttttcct atataaattg tatagcttca
      601 attatattca aagaaatgga gttgctaccc gataacttta aaagtaaaaa acagatttta
      661 ttatcttttt aaactatatt ctaacatatt ttgtccatat tgagaaattt tactaaacta
      721 ttatgtactt tattaaataa ataaaataac agcacaccta taaaatacaa gtgtgctgtt
      781 cataaacaga ttttataaaa aaataattcc taagtagaga ttaacatgct ttctcatact
      841 ctcatatata atgtgtctaa aaatattaat aaaaatgctt taattgtttt aacttacttg
      901 gatgtcttaa ttttcttatt gctttagctt ctatctgtct tattcgttct ctggtaactc
      961 caaatacctt tccaatctgt tctagtgttt tagggtcatc atcatcaatt ccaaatctca
     1021 ttcttaaaac ttgctgttct tgctctccaa gacctgtaag tagttcttct atcttttctt
     1081 ttaaattatg ctgttcaact tcgtgtatta caacatcttt aaaatcagca tctgatggta
     1141 taaattcaac taaactacta tcctcatctt cttttaatgg agtatccaaa gatacaacat
     1201 ttttatctct tcttaataat tccaacactt tgtcaacttc taagccacat gcatctgcaa
     1261 tctcatctat agttggctct cttaataata catttaaaag ttcttgtttc tttttcttta
     1321 caaaatttat tctctgatga agatgtatag gtatcctaat agtattttcg caatcgtcga
     1381 tatatcttgt tatactttgt tttatccacc aagttgcata agtgctaaat ttgtatcctt
     1441 ttttataatc gtacttttct acagctttta taagtcctat atttcctgct tgaattaaat
     1501 ccaacatatc aatactatct cttttatatc tctttgctat gctcactacc aacctataat
     1561 ttgaatttat aaatttttct tttgctactg aagatgagtt gattatttcc ttagccagct
     1621 ctacctcttc tcctgctgac aacattttgt attcctctat ttcctttaaa tacattttca
     1681 tcgcattcga tacatttgat gaacccacac aaatataatt ttcataatta ttttcttttt
     1741 tagtatccat aatcaaaccc cttctattca tagaatttgc taaaacaaaa ataaataatt
     1801 taaacctaca taatctacaa ttacataata tcccattctt taaatgaatt gttttgtata
     1861 ttatgtatac aaatttataa aaactatacc tataatatta ttctacttct aacagcaata
     1921 tcctccttat atatataatt tattttatta tatgttgtta tattcttagt tttttgaatg
     1981 atatatgtct aaaaaaagac tgtttactaa acagtctttt tttatatata tgctcaatat
     2041 ttaattttca tgatgatgat tacatccaca tccacaattt tcatcatggt gatgatgttc
     2101 ttctaaatct tttacctgtt gttccatttc atgaaactct tttaccatta tatcataagt
     2161 tggaagagct gcctcagtat cgtattcttc ccctctccaa tcagcgtaca tcatagcatc
     2221 tccattagtt aaaaccaatc tacctgacat agttatatta tccacttctt ctaaaaattc
     2281 tatttgtttt tgtaaaactt ctccttctaa ctccacatct tcatctaaac attgcactat
     2341 cattacaggt gcataataat cttcttcatc tttaacattt acaacaatat ctaaaattcc
     2401 ttctgcacct tcttcaaatt ctgccaactc aacatttgta tcaatcatat cttgaggtac
     2461 taataaatct tctattaaat attttattac tctatctttt actaattctt ttgacattta
     2521 tttttctcct tccgattcta taacattatc ataatcattt ctatattata gtatactaca
     2581 ttaattgttt tttcaatctc ttacaaaaat tattttttat tatcttcctt atattccctt
     2641 ttttattaca aagttgtaat cgcattgatt taaaaaatag tttatattta gtataataga
     2701 aatgtagcac atatttttat tcaatatatt aataaaaaaa tatattgaaa atgtaatgta
     2761 tgttaaatct aggaggaata ttatgaagtt taaaaaacaa attagttcta ttgttgtgtc
     2821 tactatgctt gtacttggga gtatgaacct ttcttatgca gacggtacaa atgtagttac
     2881 acttggagct aacttatcag agtctcaaaa gcagcaaatg cttaattact ttggagtaaa
     2941 aaaagaccaa gtcttagttt tagatgtaac aaatgcagaa gaaagacagt atttacaagg
     3001 tgttgcaaca gagggtcaac ttggaaaagt tactatctct tgctcatacg ttgaacctac
     3061 taaaaaagga aatggaatta atgttaaaac agcaaactta acttgggtaa ctagttcaat
     3121 gattgcttca acccttacaa cagctggtct tgaagatgca aatgttattg ctgctgctcc
     3181 atatcctgta agtggtactg gagcattgac aggaatcatg aaagcatttg aggatgcttc
     3241 aggaaagaaa cttgatgaaa ctaagaaaga aattgcttct gaagaattgg tagttacagg
     3301 tgacttagga gatgatatag ggcaagaaaa agcaacaggt gtaatgaatg atataaaaac
     3361 agaaattgta aagaatggta caaaagatac caatcaaata gcaaatacaa taaataatgt
     3421 tgtaaataat tacaatatta ctattacacc agaacaaaaa gaacaaatta aaggtgtaat
     3481 gaaaaaaata gctgaccaaa attacgatta caatagcatg aaaaatacac tagataatgt
     3541 atcagataat gtaaatgaac aattaaagaa attaggtgaa agtgttaagg gttctggtat
     3601 actagatact attggtggat ggtttagtgg aataggagac tggttctctg gtttattctc
     3661 aggcggagat aaaaaagatt tgggaatctt aaataatact aatgatgagt ctcttggctc
     3721 tgatgctgta ataaattcta cagaaggtgt tcaaataaat ccacaaaata atactaattc
     3781 taacaatgat agtgatacaa atagctcaaa aaatgatact tcaagtaata atgaaaataa
     3841 cgatagttct caaacaaatg aaaacaacac aacaaataat tcagaaaatc aatcaaataa
     3901 taatgactca gttggtttct ttgaaaaaat aaaaaattgg ctttcaggct tattttagaa
     3961 attaaacaaa taaaaagaat tgcctaaaat ggaaattttc tataaaaggc aattctcttt
     4021 atttacaata aatttttatt actcactcat ttttttagat ttttctatac atgcacatat
     4081 tgcttttatc acagaagctc taaaaccttc ttcttcaagt actttaactg cttctatagt
     4141 agttccacca ggtgaacata ccatatcttt taattcacca ggatgttttc ctgtctcaag
     4201 taccattttt gcagaaccca taacagcctg tgatgcaaac ttatatgctt gtggtcttgg
     4261 cattcctgca attactgctg catctgctat agcttctata aataaaaata catatgctgg
     4321 tgcagaacca cttgcaccaa ttacagcttc tataagatac tctggtacta cctcagtatt
     4381 tccaaaagaa ttaaatactt ctgtaactgc ttctaagtct tctttgttta tatttttatt
     4441 tatcgaaaga gaagacatgg cttcatttac aagagctgga gtatttggca tagttctaac
     4501 tattttttta tcttctccta taatagactc tatatctttt atagattttc ctgctgctat
     4561 tgtaactata attttattgt catctataaa gtcttttatc tcttccaaaa tatcatcata
     4621 tatatctggt ttaacagcta caattactat atcagaggtt ctagttactt ccttcgagtc
     4681 ttttgtagta tttattccaa aatttgcatc tattctatcg agagttcctt gactatatgc
     4741 tgatgctata accatattag gttcaactaa ttttgaattt actattcccc ctatcatagc
     4801 gcttgccata tttcctgcac ctatgaatcc aatttttttc actttagtac ctccttattg
     4861 actaatagta atcgttaaaa tttaagcttc tttaactgca tcagattttt caaatggatt
     4921 tggtatactt actaccaaaa ttttcactac cttattagta tagttgaccc aattatgatt
     4981 gttcttttca ctattatagt gtattgtgtc tcctggatac atttctattt gttcatctcc
     5041 caaaaacact gtaagagtac cctctaatac atacacaaat tcctcacctt catgtacgta
     5101 acaacaaatt tcctcactac tcttacttgg aagaatttca attaatcttg gtagcattgt
     5161 tttttccttt aaactagatg ataaatgata atgtataaat gttgagtttt ctacatcaaa
     5221 tacttctttt tcataacttc ttaatacagc tcttttatgc tcttttggct tcataaaaaa
     5281 gtaagttaat tctacatcta gtacacttgc tatttttcct aaggaatctg ttgccacact
     5341 tgttaatcct ctttcaagtt gtgataaaaa accaattgac aagtttgttt tttcactcat
     5401 atccttcagc gtcatttgct tttgagttct gagttgcttt atttttgctc ctatgtcctt
     5461 attcaaaacc atcaccccta aataatttta attttttcat aatataaata caaaaatata
     5521 aatataggta ctgaacccat ctttatttca tttaaaaaaa tttttataag tattactata
     5581 atttatcaat ctttttttgt ctacattttt acgattattt acgatttatt tttcacatat
     5641 gtatatttaa tcttgccaac taaagataca ttttttataa gcatttactc tttatataat
     5701 aaaacttaag ttgttcatgt agtcttcttc tggtttaatt attaaaattt caaattatat
     5761 tattatttct acacattatt acatccttta gtacaattat aacataattt tacaatttaa
     5821 tgatatctat tcaatgtctt tttttactct acaacatatt ttattttaga gtttaatact
     5881 atatatattt tttatataca gccttgtcat tttttagatt gtcaagagtt attgaagtat
     5941 tttatgtaga tttagatttt atttcctaat ttctcattta acaagaattg cttatgataa
     6001 aaaataatat catctcaata agattcttgt aaatatatct atattctaaa tataatttct
     6061 ttttttgtca aaatttcttc attctttaat aattaattta ttacttataa aataaactaa
     6121 ttatctatac aaattttata atttttaata aaatacacaa aaa
//
LOCUS       DAEOSP010000197         6153 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.201, whole genome shotgun sequence.
ACCESSION   DAEOSP010000197 DAEOSP010000000
VERSION     DAEOSP010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.201"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            186..473
                     /locus_tag="KRH54_003071"
     CDS             186..473
                     /locus_tag="KRH54_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580705.1"
                     /translation="MKKFLYALYSFIVIIANFRLKEKNYNFILLAIFFILLLIQNGVL
                     KTGYQNRATFYQDNYFNGEDKNDKIKFMTIKIFIFLSLPLCFNILFNYLTS"
     gene            complement(1012..1563)
                     /locus_tag="KRH54_003072"
     CDS             complement(1012..1563)
                     /locus_tag="KRH54_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8580706.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1754..2716
                     /locus_tag="KRH54_003073"
     CDS             1754..2716
                     /locus_tag="KRH54_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBG8580707.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            3010..4854
                     /locus_tag="KRH54_003074"
     CDS             3010..4854
                     /locus_tag="KRH54_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBG8580708.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFENSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVSISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEKIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            5176..5760
                     /locus_tag="KRH54_003075"
     CDS             5176..5760
                     /locus_tag="KRH54_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBG8580709.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         2408 a          599 c          938 g         2208 t
ORIGIN      
        1 ttataaatag attgaaattt atgaatattc atactataat ttaaatataa ggagatgccc
       61 tttgaaaata aaaattaaaa aatatttaat gctattacaa taggaactag aactacactt
      121 aataaatata cagaaattag atttggctca atataaaata caaatataga atttaggtgt
      181 tttttatgaa aaaattttta tatgctttat atagtttcat tgttattata gctaatttta
      241 gattaaaaga aaaaaattat aactttattt tattagcaat cttttttata ctgctgttaa
      301 ttcaaaatgg agttttaaaa acaggttatc aaaatagagc tacattttat caagataatt
      361 attttaatgg agaggataaa aatgataaga taaaatttat gactataaag atttttatat
      421 ttttgtcatt accattatgt tttaatatac tattcaatta tttaacttct taaattatat
      481 ttagatatag gagtcttatg ataaaagtaa gcttatgtgt tatttgtagt aaatataaag
      541 tgcatatagt ggtaaaacaa aagtttcata taaatgaaga aaaaaatata aaataattta
      601 tttaactact taaaaataaa ctaaaaggga taataaaata gcttttaggt tatggtaata
      661 aaggtattta aaaagagtgc ataagcactc caattgtgag aaatacatac acaaatcatt
      721 attacaacaa tgaaaaatgt acatataaga gacgttttgt atttgtttta agtgataata
      781 ttttaatact aactaaataa ttatcacaat agtaaaaaag aaaaaggagt attttgtact
      841 ccatttgtca aaaatataaa acttttatat aagcttatta taacataatt gataaaagtg
      901 taaagatata taatgaattt ataaagaaaa ataaaataaa aaagataaat cctaatcaaa
      961 ggatttatct ttttgtatag aaaataatta ttctattatt aaatggcatg gctaaactgg
     1021 ataataattt attattactg ctttcaatgt tttttctcct tgattatggt atgtatgaat
     1081 tgattcagct gaaaaacaaa tagagtcatt ttctctaagt tgataaatag agtcatttac
     1141 ttctaatttt aattgacctt ctataatcat aatatattcc tgtgattttt cagaatgacc
     1201 aacagatgta taactatgac cttcttctaa ttccatttga aataattcga agtttctagt
     1261 tggtgtattg ggataataac aataaagacg ataatcttta ttttctgaaa cttgtacatc
     1321 tatatcagtt tttgatacaa taaaagtttc attttgaggt tgttccagta ttgcagtata
     1381 tggaacattt agtccacttg caattttcca aattgtgttt actgttgggt ttgagtctcc
     1441 cttttcaatt tgcgaaagca ttactttact tacgccagat aattcagcaa gttgacctag
     1501 acttaagtta cgttcattac gtaatctatt taaattttta gcaataacag tattaatact
     1561 catagtagct ccttaaaatg taaattattg acaaatataa tttacgaatg tataatttat
     1621 ttaacattat ttataattta caaatgtata atataatgaa cgttaaatac actgtacatc
     1681 cgtaatataa attatactgc ataaagagtt gtttttcaac ggatgatagt tgagagatta
     1741 agggggaatt gtaatgcgtg tacttaataa tgttacagag gctataggca atactcatat
     1801 gttacattta tccaaaatag cagatgaatt tggagttcaa ggtaatattt atgcaaagat
     1861 ggaatactta aatccgggat ttagtaaaaa ggaccgtgta gcattacaaa tgattaccga
     1921 agcagaaaat gcaggtttat tagttccagg tcaagcagtt gtggaattaa caagtggtaa
     1981 tactggaaca ggactagcaa tagcttgtgc atgtaaagga tataaattta tagcgtgtat
     2041 gtcaaaagga aattctatgg aaagagcaag aatgatgaaa gctttaggtg ctgaagtagt
     2101 tcttgtagac caaatgccta attcagttca tggacaggta tctggtgaag atttggcact
     2161 tgtagaaatt gaggctcaaa aaatagcaaa agaaagaaat gcatttagag cagaccaatt
     2221 taatttagaa gcttgtaatc atgcacatga aaagtataca gcagaagaaa tgtgggaaca
     2281 attggatgga aatattgatg tatttgtaga ttttcttgga agtggtagta cttttgcagg
     2341 atgttcaaaa acacttaaaa aacataattc ttctatcaaa tgttatgttg ttgaacctga
     2401 aacagcagca atttattcag gtaaagaaat aactgaccaa gggcataaaa tacaaggtgg
     2461 aggatattct atggatttgc cattagtaga caagaaactt attgatggtt atattcaagt
     2521 tacagacgaa gaggcaactg atgtagcaag aaaattagct aaattagaag gtgtatttgc
     2581 aggattttct tcaggggcaa atgtatgtgc agcaattaag ttgttaaaga gcagtgaaaa
     2641 aggaaaaaat atagttttaa ctcttaatga tagtggttta aaatatttaa gtactgattt
     2701 atatgaagaa ttgtaataat agcacaatag ttatgcaatg ttttttaaat taagtatata
     2761 gatttaaatt ataaaaagta tctcaatgtg atttttgaaa gttataaagg agatactttt
     2821 ttattttgta aatatggagt atcctactta taagtattaa gctatataga tattgatgat
     2881 tagctatgca ataaaaaaga gtatttgttt tgcatttata catcataact tggaataata
     2941 catattaatg ttctaaatgt atttaaaaca tatgataaaa ttagaacaaa cagatacaag
     3001 gagataaaca tgcaaaagag aattttaaga aattttatat ttacgggtaa aaatatttta
     3061 agtaaacttg tttttataat aaaatataat tttagtacat taattttatt tgaattattt
     3121 cataaaggat tagccttatt ttttatatgg ccatctatta gatatatatt tgatatttta
     3181 ataaaaagtt tacatatagt ctacctaaat atggataatt ttataaaagt tttgaccaat
     3241 cccatgtcaa tagttctgat aatactatca gttataatac tagcttttta tgcatttttt
     3301 gaatttacct ctgtaattat ttgttttaat aaatcgataa aatatgaaaa aatagggtta
     3361 tttgaactct ttaaaatatc atttgagaac tctataaaat tgttatatcc taaaaatata
     3421 ctattattta tatttgtgat attaataatt ccattagtaa atacagtttt aatttcaggg
     3481 tttataggaa aaatacaaat accagagtac atactagatt atataaagtc tgatataatt
     3541 ttaaatataa tctatatgat ttttctttta ataatatata tttcggttat tagatggatt
     3601 tttagcattc atgagataac tttaaataca gaaaatttta aaagagcaag aaaagaaagt
     3661 gttaatctta taaagggaaa gattataaga acaattattt attcattaat attatcttta
     3721 gtagtagcta ttattggata tgcgatttat catagtggaa ttatatttat aggcatatgg
     3781 actaaatatt gtactgatat ggaatttttg aagaatgtat ttataaatag atgtattgca
     3841 tttgaagaat attttagatt gattttgtca atattcattt ttatattgga cattggattt
     3901 atctcagcaa cttattatca atataaagga gtaagtattt ctaaaaataa tattaaaaaa
     3961 gagaaaaaat ctatatcaaa aattgttttt aatttagtat taatattatt aatttcttat
     4021 atagagtcag ttgcattttc ttcacatata aatggtatgt ttaatacatc attttcattt
     4081 tatacaacag caattgctca tagagcagga gctgaaatgg ctccagagaa tactttggca
     4141 gcaatagaag aagcttcaca tgctaaagct gattatgcag aaatagatgt tcaagaaact
     4201 aaagatggag aacttattgt acttcatgat tcaaacttta agagaactac aggagtagat
     4261 aaaaatgttt gggatgtaaa ttataatgaa gtaaagacat atgatgcggg aagcttttat
     4321 tattatggaa aaggttatta tgatgaaaag tttgttgggg aaaagatacc aacactcaaa
     4381 gaaaagataa aatattctaa aggtaaggtg aaattattaa tagaaataaa acttaatgga
     4441 catgaaaagg gagacgtagt aaaaaaagtt gttcagttaa ttaaagaaaa taattttgaa
     4501 aatcaatgta tagttgcatc tatggataaa actatattac aaaaagtaaa aaaactggat
     4561 gagaatttac tgacatgcta ttttatggca attgcatatg gtgatttttc aaagttaaat
     4621 tatgttgata tatatggaat tgaatcaact tttgttaata aaacagtagt agaaaagatt
     4681 cataaaatga acaaaaaaat cttcgtttgg actgttaata gtgacagttt aattgagaaa
     4741 atgcttgacc taaatgtaga tagtattatt acagataatc cataccttat agagagtgca
     4801 atttattgga agaaaaatgg atttattagt caagtgtcag attatttatt ttaaatattc
     4861 tataattgta ttatccagta aaagagatat tattaaaatc aataatatct ctttttaaca
     4921 taaataaggt tgttaaatta aggttattct tataaaaatt gtgcttatca gctataaaca
     4981 aggaataaaa gtgaaaaaat tgctctgaga ttattacagt taaaagagtt ttaagatgac
     5041 agagagatag attgtcaact gtatatttaa taaatcttgt tatttattaa attttatata
     5101 agtaaaatat ataaattaat aaatcttatg ctagaatata tatgaaaaac aatataaatt
     5161 tggaggatta aaaacatgac agagcgagaa aaaatgttgg caggagaact ttacgattgt
     5221 ggtgatattg agttgttaac tcaatggcat aaagcaaaag acctagtaag agattataac
     5281 caaacagatt cctcggattt ggaaaaaaaa gaaaaaatat taaatgagtt acttggtggt
     5341 aagggtaaaa acttatggat tactgcacca tattatgtag attatggaaa taatatttat
     5401 tttggaaata attgtgaagt caatatgaat tgtacgtttt tagatgataa taaaattatc
     5461 attggggata atgcactaat cgcaccaaac gtacaaatat atactgcatt tcatccaact
     5521 aatgctggtg atagatttgg taatgccaaa gaagatggtt catttgaatt ttgtaaaact
     5581 caaacagctc cagttactat tggagataat gtttggattg gcgggggtgc tattatcatg
     5641 ccaggtgtta ctattggaga taatgtagtt ataggtgcag gaagtattgt tacaaaagat
     5701 attccaagta atatgattgc atacggaaat ccatgccgta ttattcgaga aaataaataa
     5761 agttaaattt tcttgcaaaa tctcaaaaaa tacctttctt actttataca tagggtattt
     5821 tctaatactt tcacatccat attcatttgt taaattttta tggaatgtag tttttatcat
     5881 ctctatctta cattttcact atttttttat aaaagtcatt cctttataga tttatttata
     5941 atagtatata ttctatttga tgtttgaata agcacttatt tttaagtaaa atactaactt
     6001 ttaggttagt attttgctat agtatgatga ttaagtaaga ataaaatact ataatacata
     6061 aagcttaaca aatataattt ttattttact tagatatttt cttttgatta gcaatcaaaa
     6121 gtggttataa tttttttaat taataagagt aac
//
LOCUS       DAEOSP010000198         6149 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.202, whole genome shotgun sequence.
ACCESSION   DAEOSP010000198 DAEOSP010000000
VERSION     DAEOSP010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.202"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(76..1185)
                     /locus_tag="KRH54_003076"
     CDS             complement(76..1185)
                     /locus_tag="KRH54_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG8580710.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMLMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            complement(1377..2729)
                     /locus_tag="KRH54_003077"
     CDS             complement(1377..2729)
                     /locus_tag="KRH54_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBG8580711.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            complement(2874..4202)
                     /gene="ssnA"
                     /locus_tag="KRH54_003078"
     CDS             complement(2874..4202)
                     /gene="ssnA"
                     /locus_tag="KRH54_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBG8580712.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKVKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            complement(4230..5813)
                     /locus_tag="KRH54_003079"
     CDS             complement(4230..5813)
                     /locus_tag="KRH54_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBG8580713.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAARSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            complement(5829..>6149)
                     /locus_tag="KRH54_003080"
     CDS             complement(5829..>6149)
                     /locus_tag="KRH54_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG8580714.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGTEGYYGILKS
                     VYKK"
BASE COUNT         1765 a         1251 c          736 g         2397 t
ORIGIN      
        1 tgtttgttta tctgatatat tttattatcc taatacctat tactctaagt taattaccaa
       61 aattctatat aattattact cattcatagc ttctacttga ttgtagcaga tgcttaaagc
      121 tccttttgga catctagtta cacataatcc acatccaaaa cacttgtctg catctatgtt
      181 caacttatct gtaacttcta aagcttcgta tacacagctt gttacacatt gcttacattt
      241 gatacacttg ttatcatcca ctactggagc tactgaatga gttctaaacc ctctagctgc
      301 cattttctta tgagttaatc cttttatttc attaacatca gcatatccat tctcatctaa
      361 aaattcattt aattctttag caatttttcc atagatagaa ggccctttaa ggattgcttc
      421 tgtacatact tgaacagcac ttgctccagc catcaacatt tcagccacgt cttttccaca
      481 agtgattcca ccaacaccga ttattggtat atttacaact tgagaagctt catatataca
      541 tcttattgct agaggtctta ttggtgcacc tgataaccat ccgtatccag ctttacttcc
      601 cattataggg tatcctgtat ttacatctat tgccatacat ggtccaaatg agtttatcat
      661 tacaagtcca tctgcacctg cttcttcaac agcttttgca atagtttgaa tgtcagtatg
      721 aggactcatt ttcataaata ctggtacatc taaaacagct tttgcagctt ttaaagcatt
      781 tactattggt gcaacgtctg tacctacata atgagtagat aattcaactg catctgcata
      841 aggctttact aaaggagcaa cctcagctat ttgctctgct gaatatccca tacttactat
      901 tactggaagc cctgtttctt ttaccttttt gtattcttcc tctatccatt gctcttttgg
      961 aagctctgac cataattctg tgtttaaaaa accactatta gtttttgcca tacatggtct
     1021 tggtacatca gctggtaata cagatatagt ttttgtacat atagctcctg ctccaccttt
     1081 tgctgcctct tgacagagag caccatcttt tgctccaggt cctgccgctg tcataattgg
     1141 gtttaaaaat tccattccat ataaattaac ttttaaatta gccatattat tatctctccc
     1201 ttaaattaaa tatttttaat atttagattg tattctttta tttgtattcg ttatttattt
     1261 acttttttat attatttaaa atataagata gttttgttga attattaata tctctgaatt
     1321 atttttatta atacttaatg actttaagtc aatatagatt tttacttgtt ttatttttat
     1381 tttgcttctt caactttatt atttttttct tttttagaac ctagttcatt gaagaatatg
     1441 tttaaaagca ctgctgataa actacctgtt gttattccac tatggaatat tgtttgtacc
     1501 caagttggga aattatgata gaaatcagga tttgttagtg gtatcataga caaaccaaca
     1561 ctaactgcta ctataacccc atttttagtt ccatcaaatt ttactttgct taaagttttt
     1621 actcctccaa ccattaccat tccaaacatt gcgaatccta cgcctccaag aacaggctta
     1681 ggtattgctc ctattattgc tgcaaatttt ggtaagaatc caagtactag caatataacc
     1741 cctgcgtaaa ctgctataaa tcttgatttt atacctgtaa gacctactat tcctgtattt
     1801 tgaccaaatg gagttattgg aaatgaattg aatattccag ataatgctgt agatattcca
     1861 tctccaaata gacctctttt aatattttta cctgttactt ctttgtctac catttcatga
     1921 atagctatca tattaccaac tgattccgtc ataagtacca acataatcaa acacaatgat
     1981 agtattgcag atggttcaaa tctaggcatt ccaaatttta aaggtacatt taagttaagc
     2041 caaccaactc catttactgc actaaaatct gccattccaa ctgccatgga aagtagtgta
     2101 ccaagtacta tacctaccaa tatagctgtg ctattccaaa ttccttttaa gaatttaaat
     2161 gaaagtaaag ttattgccaa tactgctaaa gctaatgcta atgagtttgg ttcaaccgct
     2221 tctaatttgt tgtcagttat ccattttatc ccaactggaa atagtgaaat acctattacc
     2281 ataacaactg ttccagtaac tacactaggg aaaaatctta ttaatttttc aaatactgga
     2341 gcaagtaata tacaaaacaa tccagctact attacagagc caaatactgt ctgtattgca
     2401 acttgtgaat cactatatga tgaggctatt gctgtcatac ctgccacagc ggcaaaacta
     2461 gttccttcta ttactggtat tttagcacct acaatatttc ctataccaac actttgtatg
     2521 agtgttccta tacctgccat aaacaagcaa gcatttatta aaaaaacaat actagcatga
     2581 tctagtcgtg cagcacttcc tatcatcata ggaactgcta ctgctcctgc acacattgct
     2641 aaaacgtgct gtagtgaaaa tacccaagat ttggatacag gtaacctttc atctactgga
     2701 gctattttag ggcttttatc tcgagacatt ttgggtctcc cttctatatt taatttaata
     2761 ttctattccg tatatttttt atttatatac ttcatttaaa ttcctatttt tgtttaagct
     2821 ctgtttttaa catctaaatt cttgtttctt acttgtatat taatatcaat tatttaaatt
     2881 ctattccata gattttgact taattctcta ctttttgcta gaatactttc ttcatctatg
     2941 tttattaact ttctatcttt cataagtact tttccattta taatagttgt atctacgctt
     3001 cttccactca ttccaaaaat agtgtgtccc cctattgtat tttctgtcaa tggagtatgt
     3061 ggtatataat caactactat aacatctgca taagcacctt ttttaagtac tcctaggtca
     3121 tttttataat aagctttagc tatttttcta ttgttatcaa ataacattgt ttgtgattct
     3181 tcaaatccaa ctcttgggtc ttttaagtga tgtttgtgaa ttatattggc aacttttaat
     3241 gattcaaaca tatcgctagt atatccatca gttcctaatc ctactgtaac tccccttttt
     3301 aataactcta ttgcaggaga acatccaact gcatttccca tattagattc tggattatga
     3361 actacaaatg tatttgtttt atttaaaaga ctgatttctt ctgcacttat atatatacaa
     3421 tgaactgcta ttgttttttc ttttagtact ccaaaatcat tgaatctttc taaaaccctt
     3481 tttccatatt tatctacatt atattgtaag tctgctagtc cttcagcaac atgtacatgg
     3541 taacctgtat caagaccagc catctctgaa gcacatttat ttaatgtatc atcagatagt
     3601 gtaaatgcag catgaagccc aaacattcct tttatcatgt ctgtatcatc tttttgagcg
     3661 tattttataa agtttacgtt ttcattgata ccttgttgga ttgtatttcc accatctcta
     3721 tcagacactt cataacaaag acacgttcta attcccaaat cctttgcaac atctgatatt
     3781 gtaaacaaac tgccttcaat tgcatttgga cttgcatgat ggtcataaac tgttgttaca
     3841 ccatttttta tacattctat atacgttgca tatgcactgt attttgtatc ttccagtgta
     3901 agctgtttgt caagtctcca ccataaattg tttagtatct ctaaaaagtt ctcagaaggt
     3961 tttcctggaa ctgccattcc tctagcaaat gagctgtata tatgcatatg tgaatttatt
     4021 agacctggca tgattatttt cttgcctgca tctataaatt catgtactgg atatttaact
     4081 ttcatttctt ctgtagtacc tatatcttca attagattat catttattac tacacatcca
     4141 tcttccatat agggatttaa agaatcttgg cttattagcc tcccattccc cactaaaatc
     4201 ataaatttat tcctccttaa agctaagatt taatttttaa gtacttttcc tgctttaatt
     4261 ttattgtatt ttccttcctc aacaacacaa tttccattta taattacgta atctatacct
     4321 gtagggtact gcatagagtt gataaaatca tcacaaccac taactgtatc cttgtcaaat
     4381 ataactatat ctgcaaaata tccttctttt aattcgcctc tatcttttat actaaagctt
     4441 ctagctgcct ttttagtcat tttataaaca gcttcttcga ttgttaacac attcaactct
     4501 ctaacatatc ttcctagtat tttagggaaa gaaccataag ctcttggatg tggtttacct
     4561 gctaaaagac catctgtaca cacattttgc tcgtctcttt tcatgaatcc tattatatgt
     4621 tcttctaatc catagaaatc taccatccca actgcatttt cttcttcttt taataaatca
     4681 aacgttgcat ctaatgggtc ttttcctctc atttttccaa tttctaataa gctcttacca
     4741 attgtatctt tattcttttc tgtttttacg cttgttacaa atatttggtc tattccagca
     4801 aattctataa aattatccca gccttctatt ccatttgcta tatctttttt catttttgct
     4861 ctttcgtttt catcacttaa tctttctatt aacttatcag ttcctccaga atgtgcccaa
     4921 ggaggtaaaa ctactccaag cattgtactt cctgctgcat atggatattg gtcaaaagat
     4981 actcttattc cttccttttc tgctttttct aaaagctcta taacttgtgg tatatacttc
     5041 cagtttgctt taccacaaac cttaaaatgt gaaaaatgaa cttttactcc tgattgtttt
     5101 ccaatttcta tgatttcttc cattgaagtt aaaattgtat ctgcttcact tctttggtga
     5161 actacaaata ctccgtcata ttttgctact accttacaca tctctataat ttcttcagtc
     5221 aatgagtatg cacaaggtat atatataagt ccagtagaca atcctatagc tcctgctttt
     5281 aattctctct ctgtgatttc acacattttt tgaatttctt ctttagtagc aggtctatct
     5341 tcaagtccca tagcttccat tctaacgttt ccatgaggta ctaaataagt ttcatttaaa
     5401 cctaccccat tgtaatccat catttttaaa taattctctg ttgtttcata tttccaatca
     5461 atttcatcac tttcaccatc taaacctgct aaattttttc tccaagaaga tatatgctct
     5521 tgaggtagag gggccataga tataccatct tggcccaaca cttctgttgt aattccctgt
     5581 ctaatcttta cctcattata tggattaact aaaataacta ggtctgaatg gctatgagta
     5641 tctatgaaac ctggacatat aactctacct tttgcatcta taactttgtc agcttcttcg
     5701 tttaaatctt gtgatatttt taatattttc tcgttttcta ttaaaatatc tccttcatat
     5761 gctggtgttt tatttccatc tactataagt ccatgtttta ttaaagttct cacaaaacca
     5821 tctcctaatt attttttata aactgatttt aatataccat aatatccttc tgtacctttg
     5881 aataattggt ctaattctat atactcatct attgtatgag caagattttc cttagaagga
     5941 ccaaatccta tagttcttat tccagcctca cctgcgtagt gacttccatt cgtacagaat
     6001 gagtattgag ttatttctga atctattcca gcttctttta atcccttata cgctgcttga
     6061 acaaattcat cttcttcatc atataaccaa cctgggaaga atctttcact ttctatagta
     6121 tttcctgtat agcaatcagc tttttcaac
//
LOCUS       DAEOSP010000199         6133 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.203, whole genome shotgun sequence.
ACCESSION   DAEOSP010000199 DAEOSP010000000
VERSION     DAEOSP010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.203"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(36..1133)
                     /gene="ychF"
                     /locus_tag="KRH54_003081"
     CDS             complement(36..1133)
                     /gene="ychF"
                     /locus_tag="KRH54_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBG8580715.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
     gene            1449..2714
                     /locus_tag="KRH54_003082"
     CDS             1449..2714
                     /locus_tag="KRH54_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG8580716.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            2729..4015
                     /locus_tag="KRH54_003083"
     CDS             2729..4015
                     /locus_tag="KRH54_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBG8580717.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDRLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            4409..5155
                     /locus_tag="KRH54_003084"
     CDS             4409..5155
                     /locus_tag="KRH54_003084"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBG8580718.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            5185..6120
                     /gene="rsmH"
                     /locus_tag="KRH54_003085"
     CDS             5185..6120
                     /gene="rsmH"
                     /locus_tag="KRH54_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBG8580719.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
BASE COUNT         2372 a          652 c         1055 g         2054 t
ORIGIN      
        1 atatataata gtcaaagtgt atacgattaa tcattttaaa cattaaatct aaataatatt
       61 acatctccat ctttaactat gtattcttta ccttctaatt taaccaaccc tttttcttta
      121 gcagcagcca tagtaccatg ctcaaccaaa tcattaaagg ctattgtttc tgctctgata
      181 aatcctcttt ctatatcaga atgtattttt cctcctgcct gaggagcttt acttccaact
      241 tttatagtcc atgctctaac ttcttttgga ccagcagtta agaatgaaat taaaccaagt
      301 aaagcataag aagattttat aagcttatca agccctgatt gttcaagacc taatgtttct
      361 aaaaactctt tcttttcttc agcagaatca agttcagaaa tttctgcttc tatttgtgca
      421 catactacaa caacttcggc atcttctgtt tctgcaaatg ctttaacttg ttgaacatat
      481 ttattatttt ctccattatc agctaaatca tcttctgata catttgaagc atatattaca
      541 ggcttagatg taagtaaatc caatgaattt acgaatgttt gttcatcttc tgtaaattcc
      601 atagttctta cacacttact ttcttctaaa gtagacatta ttgattttaa taaatcaagt
      661 tcagaaccta aagttttatc agccttagca gctttttgag ttttattaat tcttctttca
      721 agaatctcta tatctgagaa aattaattct aagtttatag tttctatatc acgaagtggg
      781 tctacagaac catctacatg tacaacattt tcatcctcaa aacatctaac aacatgaact
      841 attgcatcaa cttctcttat atgtgataag aatttatttc ctaaaccttc tcctttagaa
      901 gctcctctaa ctaaacctgc tatatcacaa aattctatag cagtaggaac tattttttca
      961 gaattatata attcttgtag tttgtttaat ctttcatctg gaacagatac aactcccaca
     1021 tttgggtcta ttgtacaaaa agggtaattt gcagattctg cacctgcttg agttattgcg
     1081 ttaaatagtg tgcttttacc tacatttggt aaaccaacta tacctaattt catattaatc
     1141 gtcctctctc ttattctttt ttatattctt tatataactt taaacaatca atctatagga
     1201 ttgcaaaatt gttttaatta tagacataca tgttgattat acatcatata tatcaagctt
     1261 gtaaactaga ctttaaagca tattgattta aataatattt gtatttaatt taaataaaaa
     1321 tattgctttt agaaaataat aaaaaggata taattaaata gaaggttttt atatgtgagt
     1381 atatgtaatt aaaaaataaa taaatttcaa ttttaaaatt gatgtttaaa taaaaatagg
     1441 aggtacatat ggttaatact aaaaaaataa taaatttagg taataatatt aatcttactt
     1501 tgataaaaac agaaaagttt aaatcgaatc ttataagttt atatgttcag agactattgg
     1561 atgaaaaaga aactacaaaa aatgctctaa taccatcaat aatagcaagt ggaagcgcta
     1621 aatatcctag tgctagagca atatccaata aattagatga tttatatggt tcatctatgg
     1681 gagctgatgc tgtaaaacgt ggagaacgtc aagtattgag ttttaaagta attaacataa
     1741 gtgaaaagta tttagatgag agtatatttg aagaagtagt agaatttttt aatgaagtaa
     1801 taaataatac gcttgttgta gatggtgggt ttaaagaaga atatttaaat attgaaaaag
     1861 agaatttaag agaaaagata caatcaataa taaatgataa aaaggaatat gcgcaagata
     1921 aatgtattga ggctatgtgt aaaggagaga gatatagtgt aagtgaattt ggttatgaag
     1981 atgaaataga tagtataact tcaagagagt tatatgaaca ttataaaaac atacttaaaa
     2041 catcacctat agatatagtt gtagaaggta attttgatga agataaagtt gtagatataa
     2101 taagtaaaaa tctaaaattt gaaagagaag aaattataaa tataccaaga gcagatttta
     2161 taaaaaatgt ggatgaggta aaagttatag atgaacaaat ggaaataact caaggtaagt
     2221 tagttatggg ttatagagca aatgtggatt atgcagacat agataaatat tatgctttag
     2281 tagttgggag caatgtctta ggtggtggtc cacactctaa attatttgtt aatgtaagag
     2341 aaaaagaaag tttatgttac tatatattct cttcaataga gaagtataaa agtatcatgt
     2401 ttatatcatc tggtatagaa actaaagatt atgataaagc tgtagaattg ataaaaaaac
     2461 aagtagaaag tgtaaaagca ggtgatatat ctgatgaaga attagaaaac agtaaacttg
     2521 ctttagttaa ttctatgaaa tctataactg ataatatagg tggtatgtct gattttgttt
     2581 tctctcagtc tatggcaaag acaaattctg aagtacaaga cataatatct agtatagaaa
     2641 aggttacaaa aaaagatata gttgaagcta taaaaaatat agagcttgat actgtatact
     2701 tcttgagaaa ttaatggagg agatgtctat ggaaaaaata gttaatgata tattgaaaga
     2761 agaagtttat tatgagaaat tgcaaaatgg attagatgta tattttatgc ctaaaagggg
     2821 ttttatgaaa aagtatgcta ttttggctac taattatggt tcaaatgact tagaatttgt
     2881 accaattgga gaagataaga aaattagagt gaatgaaggt attgcacatt ttctagagca
     2941 taagatgttt gagcaacctg atggaggaga tgcatttgat aaattttcaa aattaggagt
     3001 aaatgcaaat gcatttacta attttacaat gacagcctac ttattttctg ctactgaaaa
     3061 tttttatgag agtttagaac atctaattga ctatgttcaa acaccttatt ttacagatga
     3121 aaatgttgaa aaggaaaaag gtataatagc acaagagata aaaatgtaca atgatgaccc
     3181 tgattggaat gtatacttta attgcttgaa ggcaatgtat gtaaattatc ctgctagaat
     3241 tgatatagca ggtactgttg atagtatata taagataact aaggaagagc tatataaatg
     3301 ttataatact ttctataatc caggaaatat ggctttattt gttgttggtg acttagatgt
     3361 agaaaaagtt atagatgtaa ctaaaaagtc aaacaattac aaagtagaca gactctctaa
     3421 gagtatagaa agattttatc cagaagaacc agaaagtgta aaagaaaaag aggttataga
     3481 aaaattccct atatctatgc ctatgttcaa cataggtttt aaagatagca atgttggttt
     3541 aaaagggaaa gaacttttac gtaaagagat tgtaactgat atacttgtag ggatgttatt
     3601 taaaaaagga agtaagcttt atgaagattt gtatatgcaa ggtttaataa atgaaaattt
     3661 tggagctgga ttttcgtctc aagtggatta tgcttttagc ataatagctg gagattctaa
     3721 agagcctaaa aaagtaaaag aaataattct tgattatata gaaaaatcaa aaaaagaagg
     3781 attatcaaaa gaagagtttg agagaactaa aaagaaaaaa atagggtcat ttattaagtg
     3841 ctttgattct ataaacttta taggtaatag ttttatatct tatgttttta aggatataaa
     3901 tctattggat tatttagatg ttattaaaga tattactttt gaggaagttg aagaaagatt
     3961 aaaagagcat tttaaagaag agtattgtgt tatttctatt gtagagccta aatagaaact
     4021 taatcataat aagaataact aattaagtat tagtttatgt ttttaaatgt ttattttggt
     4081 aaaacaaatt tataaaatat atttatataa attgaggtaa aaatttaaga gctatagata
     4141 aggaatagaa gaggttgatg tatatatgca tcaatctttt tgattaaaaa atatctatgt
     4201 acacttgtaa ctttagtcat gagagattca gtttaaaaaa ttttatagta attagatata
     4261 atatagtgta gggagttgta gaataaattt taatattttt tgtagaatta acattaatgt
     4321 gttatcataa tttagtataa ttacaattaa ttgtaattat agcatattgc tttggaaata
     4381 ttttcaatta atacaaagag gtgacgttat ggatagagta gcatttacac tgtttggcat
     4441 agacatcatg tggtatggaa tacttatggc atgtggaatg attttaggaa ctttaatagc
     4501 aataaaagaa gcaaaaagag tgggaattaa agaagatgat gtattaaata tagctataat
     4561 agcaattcca gttggactaa tctgtgcaag aatctattat gttgtattta attggagtta
     4621 ctatgctcaa aatatgtctc aaatttttaa ttttagaggt ggtggacttg ctatacatgg
     4681 agggttaatt ggtggaatat tagctggtta tatatacact aagattaaaa atattaactt
     4741 cttaaaaatg gctgatacag ttatacttgg aatgccttta gcacaagcta ttggaagatg
     4801 gggaaatttt attaatggag aagcacatgg aggtgctacc aatttacctt ggggaataat
     4861 ggttgatgga gtaaaggtac atccaacttt tttatatgag tctatatggg attttggaat
     4921 atttatagtt ttattactat ttagaaaaaa taaaaaatat gaaggtcaag ttatagttac
     4981 ttatataact ttatattcaa taggaagatt ttttatagag ggattaagaa cagatagttt
     5041 aatgttagga ccacttagaa tggcacaagt tataagtcta attggagtta ttggtggcat
     5101 aattgcacat gtttatttat caaagaaaaa taaaaataat attagtgaag aatagtttaa
     5161 aaagtgaatt taagggaggt aactatggaa tttcatcatg tatcagttct tttaaatgag
     5221 tgtatagaga atttaaatat aaagccagat ggcgtgtatg ttgactgtac tatgggagga
     5281 gcaggacatt ctaaagaaat agtaaaaaaa ctgtcagata agggattatt tatagggttt
     5341 gaccaagata aaaatgctat aagcacagca aaagagagat tgagtgaata tgaaagtaga
     5401 gtgaagtttg ttcacagcaa ttttgaaaat ataaaagagg aattagaaaa aataggagtt
     5461 tacaaaattg atggtgtatt agctgattta ggagtttcat ctcatcaact tgatgaagct
     5521 gatagaggat tttcttatat gcaagatgca ccacttgata tgagaatgga tgttaggtgt
     5581 gagttttctg catatgatgt tgtaaatact tatacagaag atgaattaac taagataata
     5641 aaagattatg gagaagataa ctgggctaag cgtatagcta aatttatagt tgaagaaaga
     5701 gcaaacaaac caatagaaac tacaggagaa ttagtagatg taattaaaaa agctatccct
     5761 aagaaagcta gaatagatgg acctcaccca gctaaaagaa cttttcaggc aatacgaata
     5821 gaagtaaata atgaacttgg agtaattaca aaaatgataa atgatgcttc ttctataatg
     5881 aatgaaggtg gaagaatatg tataataaca ttccattcat tagaagatag aatagtgaaa
     5941 aatgcattta agcatttagc atcagattgt atatgtccac aacatttacc tatatgccaa
     6001 tgtgataaag aatctgaggt gaaaataata actagaaaac ctatattacc tagtgaggaa
     6061 gaaatagaag taaatccacg ttcaagaagt gctaagctaa gagtagcaga aaaaatataa
     6121 gtagttataa ggc
//
LOCUS       DAEOSP010000200         6062 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.204, whole genome shotgun sequence.
ACCESSION   DAEOSP010000200 DAEOSP010000000
VERSION     DAEOSP010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.204"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(169..1527)
                     /locus_tag="KRH54_003086"
     CDS             complement(169..1527)
                     /locus_tag="KRH54_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8580720.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGALYCLRLRGTKWLKHK
                     VA"
     gene            1975..2427
                     /locus_tag="KRH54_003087"
     CDS             1975..2427
                     /locus_tag="KRH54_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HBG8580721.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            complement(2584..3201)
                     /locus_tag="KRH54_003088"
     CDS             complement(2584..3201)
                     /locus_tag="KRH54_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBG8580722.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFIYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(3624..4436)
                     /locus_tag="KRH54_003089"
     CDS             complement(3624..4436)
                     /locus_tag="KRH54_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8580723.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
     gene            complement(4512..5930)
                     /locus_tag="KRH54_003090"
     CDS             complement(4512..5930)
                     /locus_tag="KRH54_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBG8580724.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
BASE COUNT         2090 a         1063 c          604 g         2305 t
ORIGIN      
        1 gccaaattat gtaattctgc tcaatatata atttataata tccatactat tatagccttt
       61 atttatgtaa tttaatcacc ttatatatca tacttataat tatgataata actacataaa
      121 aaatcacttc taaatagagt tatccatcta aaagtgattt catatattct atgcaacttt
      181 gtgttttagc cactttgttc ctcttaatct taaacaatat aaagcccctc taacaagcca
      241 atcagtatac atacctatcc aaattcctaa cactccaaat tctaatacaa cccccaatac
      301 gtaacctgta aaaattctaa aaatccacat acctattact gcagtcatca ttgtatatct
      361 agtatcaccg gctcccttta accctgctga taatacaaag gatataggcc atattaccat
      421 agctaaacaa tcacttcgaa ttaattgtgt tgagatgtca attacactag cattttgagt
      481 atacatactt gaaatccagg gtgcaattgg tataaatata gctcctacta taaataaaca
      541 aattgttgca aatttagtta aatataataa agtactctta gctcctttaa tatctccacg
      601 tcctatatac tgtcccacca aggttgtagc agacaaagct aaagcattcc caattacatt
      661 tattatggat gcaattgaca ttcctattgc attggatgca attgctgatg ttcccatagt
      721 aactataaat acttgaacaa tcaatttacc tgcattaaaa agtagttgtt caactccagc
      781 tggtattcct atattaaata tagacttttg agtttcaaca tcaatcttaa atggaaatat
      841 tttacccata gatataaatt cgtttccttt aaataaagct cttataatta ctatacatcc
      901 aattattcta gctatagcaa tagatatagc tgcacctgtt atgccatagg atggtgtttg
      961 caaatttatt cctagaggat ttaaatcaat accatatata agtatatatc ccaatgttac
     1021 atttactata ttcataaaca tagtcacata cataggtttc tttacatctc cacaccctct
     1081 taaaatacca tttgctattt gttgtgtcgc aataaatgga tacgtaaata atgtaaactc
     1141 tatgtataat tttgcatttg attttaccaa ttcttctgct gaaccataaa ataaattaat
     1201 tacaccagtt ctaaaaaccc acatcaatat tgttattgta aaggataata ttactccaga
     1261 cactagagcc tgcttaatag tttcattcac cttttcacta tctttttttc ctatttgttg
     1321 agctacaaca acagtagctc caacagatag tgcagcaaat acagatataa atatattgtt
     1381 tattgaatca accattccaa ctgcagatac agcttcttcc ccaatatgac ctgccatcat
     1441 tgtattaaat actcctaaaa gcatgacaaa cgtctgctct atcattatag gaattgctaa
     1501 ctttataaca tttttcctta taaacatccc tattcctctt ttccctcttt atataaattt
     1561 aatttttaat tagtttatcg aactgtaaat tgacaatgca aagtttagaa ttgtatcgtt
     1621 cgcattataa tatactgctt tcaaataata taaatcttgt ttaatatcat ttgaacacag
     1681 tatatcttta taattatctt atgtttatta ttttttatgg ataccacatt taaaatattt
     1741 attaatttaa tataaatatt ttccgttatt attaaaagta gctatatttc gttaatattg
     1801 tatcactatc gtgtagcaat aacaataagt acattttata tattttttat aatattattt
     1861 aatcatccaa acttttacca ctttttcaat atataaaata ttacacttta gatgtaacaa
     1921 aatcgcatcc atttaaatat tgtattttat agactaattt taggatgtga tactttggtt
     1981 aatttaaatg caaataatct aaatgtaaat atggtttatc ctatttcttc cacagcaccc
     2041 atacgtttca gaagatatac tgtttcatat ttagaaaaca tggaaaatat ttatttaact
     2101 atagcaacat actttgatac acttggttta ggcacaatga atatcgacaa agtttatggt
     2161 caatgggctt ggtttactga taatatatat gatttaaatc tatttgtttt tgtaggaaat
     2221 tatccttatc aaattgctaa aaacagatat gatacatttg tagatatttt gccagtagct
     2281 attacttcta ttgtcaatgg agatagagta tttttaattt caaatccttg tttgttaaat
     2341 tcaaaaatta cagttagatt tatatctagt catcctactc ttaatagaac agttgattat
     2401 ggcaatatta gagattttat tttttaaaac tgcaacatga tgtgctatta aaaaataccc
     2461 taaatttaat gatattatta aataactaga atatttttaa ttggcacatc atgcctagct
     2521 attataaatt cataatatta ttttacttaa gataagtact gtattttatc tacttgtata
     2581 tttttattta cctagtttct cagttctata tcctatttct ttattagcta gcttataacc
     2641 ttcttgtatc atttcttctc tcgttaatgc actccctaat aaattgtcaa tactcagttg
     2701 ctttctatca ctaaaatact tatccaataa tgattcctct atatcttcct gatatgccaa
     2761 atttccttca tgcattatat attttacatc aaaaccttct ttatttaata cttttcccac
     2821 caagatactt ctatgacatc ttataggttc ttgcatagct cctaataaaa ctatcctata
     2881 acccatttga caaccctttt ttaatctttc tattccgttt aaaaaaatat cttcttttgc
     2941 tactttctca aaatctgcat aaccttcttg cgtatataca tctttattag ttcttttagc
     3001 tccaaattct tttcccatgt atatatatat aaatccggaa tttctaagag attcgttaaa
     3061 tgcctcttta ttgtattgaa tattatattt agaatatgga gtttctctaa tgtcaactac
     3121 acaatttatg tcatattttt ttatcatatt aataagctta tcatatggat aatttgaatg
     3181 tcctattgcg aatatttcca tataaacacc ctcctattct ttgaaattag tatctatata
     3241 tgtagatacc cgatttactt aaaattaaat aaagtattac tttaaatacc caatatcttt
     3301 ttttcttgat attgtatatt tttttacttt atatccttaa aaaaatatac aactttatat
     3361 ataaaacatc aatatttata tatattttgt atattaaata caattattta taaagtcttt
     3421 aataaaaatt tataaataga gcatctttag aaaactatta taataaaaaa ataagaggct
     3481 gtctaaaaat atagataagc cttttttcat aatcgtaatt ataaaaatat acttgatttc
     3541 atatgaaaaa gagtgtctca taaactaaaa tgtttacttt gagacaccac cttttttatt
     3601 cttatttata ctttgtagac atattataac atgcgtttca ctggtatctg aacttctgtc
     3661 aaccagtctt cttcactctc tttattccat ataccatcta tataagattc tctaggattt
     3721 ccaaccactt tatatttatt gtcctctatc catttaaatg catctgcgta agtttttcct
     3781 aatgttgaat atggaccatt gtgtaatata caaactgcat tttcaactgc atctaattct
     3841 ttaaatttta atatatccgt atcttccttt aaatcattta tagcttgaca catttcaaca
     3901 tccatatcag tctctctata ttcaccatca tgatatatat taaaacaata atcaggaact
     3961 gcacatgtac accctgtttt catcatttct tcagccatta catttggaaa tagattaaaa
     4021 agttcatcat acccctttaa cacttttctc atagaagcaa ctttaacttt tggtaaagac
     4081 ttaataatta catcattcat attgacttct cctttcaaat tcataaggta attacgtagt
     4141 ttaattattt tatattcttc acttttaatt acatccaaca tttctttttc tttattcatt
     4201 aaaatatttt caacttcttc aggattttct acagcttttt ttatctcagc taatgataaa
     4261 cccatttgtt ttaaagttat tatctgatgt aatttaggta attgctctgt tgtataatac
     4321 ctatattttg taaattcatc tacatattct ggttttaaca accctatatc ttcataatac
     4381 cttaatgtct ttgtagttat tttatttatt ttagaaaaca ttcctataga aaacatctca
     4441 tcacctgcgc attagatttg attaatttaa gtttaaactt tccccttagg ttaaagtcaa
     4501 tacaaaataa attataattg tattaattct tcaattctat ctccactcaa taccaaaata
     4561 tcccctgttt ttataatggt tgaaccttta ggtattaaaa cctgttcctt tctttttatc
     4621 ataactatta atatatcgtc tggtatatta gaatctatta tactcttatt agcccattta
     4681 ttaccctcat caacatttac ttcgataagt ttagagctaa tttctccatc ataatcatta
     4741 aatgttttta atacaggtgt attatcatca actaaatcca actttctggc tacagttggt
     4801 atcaatgaac cttgtattcc tactgaaagc aacgctataa aaaatattat atggaatatg
     4861 tcattctcta tggctactcc ttgagtaaca gcataaattg caaaaactac tgaagccgca
     4921 cctctaagac ctacccacga tatgaataat ttttctttat ttgagaattt aaattttgct
     4981 agaactataa atgttgctaa tggtcttgca ataactatca tgaatataga tatggccatt
     5041 gctataccaa atacatttgg taacttagaa gggaaagata acaatcctaa cataaagaat
     5101 aacattattt gcataatcca tgaaattcca tcaaagaaat gaaccaaact ttttttaaga
     5161 ggtatcctac tatttcctat aataatacct gaaatgtaaa cacttaaata tccatttcct
     5221 ccaagatact cgctaaatgc ataagaaagt attgcaatag ctgttataaa tatagtataa
     5281 aatccttcta tctcgaagtt tgaatgtctt aaaatataaa cagataattt tgctagtata
     5341 aatcctatag ctaacccaaa agctatttgt tttattacca ttggtgcaat aagttctacc
     5401 ccagaattat tcatcaatgt caaaagaatt agtgtcaaca tatacgctat tgggtcatta
     5461 cttccacttt caacttctaa taatgatgca agtgaaccct ttaagtttag tttttgagaa
     5521 cgcaacacag caaacacaga tgcagcatct gttgaagctg caattgcccc aattaataaa
     5581 ccttcaagta aactagttcc tattaccatg tggcaaaaca tacctgtaac gccagccgta
     5641 attattacac ccagtgaaga cattaatata gaaggtatag caacaggttt agccatattc
     5701 cagtttgttc caaaccctcc aaaaaacatt ataaatatta gtccaataga acataattgt
     5761 tttgtcagtt catagttatc aaaatagatt cctaccatcc catcagaccc aaataacatc
     5821 cctagtaaga taaaaatcaa taatattggt actccaaatt tatacaatac cttgctagaa
     5881 gttatacaaa tcaatagtac taacccactt ataatcatta attctgtcat agcatcaact
     5941 ccatttcata taaattttgt agattaattt acatctacaa ttttattact tattcaaata
     6001 taacatgaca taaattttat aagttatggt acataaagtt caacaaaaat ttcacaatac
     6061 at
//
LOCUS       DAEOSP010000201         6036 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.205, whole genome shotgun sequence.
ACCESSION   DAEOSP010000201 DAEOSP010000000
VERSION     DAEOSP010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.205"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            121..567
                     /locus_tag="KRH54_003091"
     CDS             121..567
                     /locus_tag="KRH54_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580725.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            831..1556
                     /locus_tag="KRH54_003092"
     CDS             831..1556
                     /locus_tag="KRH54_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8580726.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            1543..2787
                     /locus_tag="KRH54_003093"
     CDS             1543..2787
                     /locus_tag="KRH54_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8580727.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFKSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGQGTEFHIIFHKMYDSD"
     gene            2867..3544
                     /locus_tag="KRH54_003094"
     CDS             2867..3544
                     /locus_tag="KRH54_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580728.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            3545..>6036
                     /locus_tag="KRH54_003095"
     CDS             3545..>6036
                     /locus_tag="KRH54_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8580729.1"
                     /translation="MNLYTLLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINNSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIITIVSIIIVLLTIF
                     ISILLPAISASNISHLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIA"
BASE COUNT         2534 a          552 c         1017 g         1933 t
ORIGIN      
        1 aaacacaaaa tataagttta tattttgtaa aaattgtaat ttaaaaaata acataatttt
       61 tattaaaata atacagatac tatttataga tagaaataaa aattagaaag gagaaaaaat
      121 atgttaacat atttaatgaa aagacaaatg cataaaaaag atgatggaat gagtaaacca
      181 atgatgatta caggagctgt aattacaggt gtaggagcat atgctgtata taaaatggta
      241 aaaagccata aatctaattc tcaaatggtc aatacaaatt attatggaaa tagcgattat
      301 gatgattata aatatgacga atttgattat gactgtgatt gtagagacac aaaacaagat
      361 tatgaatatg aagaattaaa aaaaaaagta aaagaattta attcaagaag aataaattgt
      421 gaaaattgtc cacatgtttc tcaagaagaa atgatgcaat atgtaaatag tgttaaagat
      481 gtaaaggaag agattgattt acatgaagat gctaaaaaag ataatattta caaagaagat
      541 gaatacaaaa aatatgaaga agaataagta atattttgta taactgaaca aatagtaatg
      601 tcaaattatt taatatctag ttttaatatg taagttgtaa cttaacttga aagaatcagt
      661 atcattcttt agatagttaa gtaacaactt acattttatt tttataaatt ttaatagttc
      721 tttgtgacta ttttgtaatt ttagaaatat tttagtatcc agggtaattg tgtataataa
      781 aacttgtata gatagatagt atgaaattaa gcagatagtt taaaattaaa atgaaaagaa
      841 aggaaatagt tatgttaaag ttgttgatag ttgaagatga tagtactata tcttttggaa
      901 taaagtatgc tttagaacaa gaaggattta gtatagatat atcaaaggat ttatctagtg
      961 gaaaagaaat gatttcttct aatgaatata gcatgatact gttggatgtt acattgccag
     1021 atggaactgg ttatgagctt tgtcaatata taagaggatt ctctcaggtt cctataatat
     1081 tcttgactgc ttgtgatgaa gaagtaaata ttgtaatggg tctcgatata ggtggagatg
     1141 attatataac aaaacctttt agaattagag aattaatatc cagaataaaa gctgttttaa
     1201 gaagaaaagg taatacttca gaagaaaata agaaaatact taaatttgga gatttaagta
     1261 tatatacatt agaagctaga gtatataaaa atgataaaga aatatttctg acatcagttg
     1321 aatataaact tcttttaatt cttatacaga ataaaaatac tgttttaagt agaactcaaa
     1381 tattggaaaa actttgggat gtaacttatg attttgtaaa cgacaatact ctaactgttt
     1441 atataaaaag gcttagagaa aaaataggtg atgatttatg tgaaccaatc tacatagaaa
     1501 ctgtaagggg gattggttat aaatggatag gaagtgaaaa tagtgtttct atataatcca
     1561 gaagtgaaga agtttttaag taagtatgtt actttgatgt ttattgttat cataatatct
     1621 attggattta gcataatcaa cgtgtcctta actaaagaga tggttgtaag aaataatcag
     1681 gctataatag gaactttatc aagcaagtat ccaaacttgg aaagtcagat agtagacata
     1741 ataactcaag ggaagtctat ggagaacata gattatggtc agaaaatatt aattaagtat
     1801 aattatgata aatctattag gataaactct gagcctatta tttctaaatt agttttagag
     1861 actatcaaaa taaatataat attagtatgt ataatattta ttttgatatt tgtgttggtt
     1921 gttagatatt ttaagtcaat ttacaatgat ttatcagata tgactaagta tgtttactat
     1981 agttcagagg gtaagccatt tgatatgaaa aacaagaatc aagaagggca gataggtctt
     2041 ctaaaaactg aactcttaaa gatgacaaca attcttaatg aaaaggttga acttttaaaa
     2101 agagaaaaga tatttttaaa caatacaatt tccgatatat cacatcaatt aaaaactcct
     2161 atgacttctt taataatgtt gaatgattta ttatataatg atgtaccata cgaagtaaaa
     2221 atcgattttt taaataagat aaaaaatcaa ttaaatagaa tggattggtt gattaaaagc
     2281 atgcttaaat tatcaaaggt agaagcaaaa gtgataaatt ttaaaaaaga taaggtaaaa
     2341 tttagtgaac tcatacatag agcaatgcta tctatgaaaa tacctatgga gattaaaaat
     2401 caaaaactga ctatagaggg aaatgataat gtatcttata ttggtgatat tgattggtcc
     2461 gtggaagcat tagtgaatat aatcaaaaat tgtgtagaac atactcctga atttggaaat
     2521 ataattataa cctataaaga aaatccatta ttttctgaac taataataaa agatgagggt
     2581 gaaggtatag ataaaaaaga tataccacat gtatttaaac gattctatag aggaagaagt
     2641 agttcaaaag aagatagtgt aggaataggt cttgcaatgt caaaatctat aatagaaagc
     2701 caaaatggcg atatatatgt aaacagcgaa aaaggtcagg gtacagaatt tcatataata
     2761 tttcataaaa tgtatgatag tgactaatct gtaatattta attcacttta tagtaatagc
     2821 caaaacctaa tatatgaagt atagaatata aatattggag gtagttatgg aaattttaag
     2881 agtagaaaat ctaactaaaa gttatggtaa aaatgaaaca aaagttgatg ctataaaaaa
     2941 tgtaagttta tcagttgaaa aaggaacgtt tatagcaata acaggtccaa gtggaagtgg
     3001 taaaagtacc ttattacatt tattaggagg agttgacaga ccaactagtg gtaaggttta
     3061 tataaatgat gttgatatat ataatttaaa aactaaggat ttggcgatat ttagaagaag
     3121 aaatataggt cttatatatc aattttacaa cttaattcca gtcttaaatg tgaaggaaaa
     3181 catattgctt ccagcagagc ttgacaatag agatattgat agagaatatt ttgatgattt
     3241 aatgaaaaca ttaggtctaa ttgatagaga aaagcacctt ccaaaccaat tatcaggagg
     3301 tcaacaacag agaacctcta taggacgtgc attaattaat agaccttcta taatattagc
     3361 agatgagcca acaggtaatt tagatagtaa aaattcaaag gaaatattag aattgttaaa
     3421 attgtcagta aaaaaataca accagacact aataatgata actcatgaca aaaatatggc
     3481 acttcaagct gatagaatta taagcataga agatggaatc ataaaaagtg atgaggtgat
     3541 gtagatgaat ttatatacat tactaaccat tagatactta aaacaaaata aaagaagaac
     3601 cattgtaacc ataatcggta taatactttc aacagcttta atttgtggta taggaaatat
     3661 atttgaaagt tttatggatt atcaaatgag agaaactata aagaatgatg gaagtttcca
     3721 tgttacattt tatgatgtaa atagaaaaaa tgtggaatat gtaacgaaat cagctgaaat
     3781 agaaaaacat gcatttacca aacaacttgg atattctaaa ttagaaaata gtgaaaatgc
     3841 tatattaagt ataaagcaat atgataaaaa tgcaataaat ggatataaag tttctataaa
     3901 agaaggtaga tttccagcta aggaaggaga aattgttcta agtgaaagta ttattaatct
     3961 tatagatgat aagttaaaaa taggagataa gataacttta aaagtagggg atatgtttga
     4021 ctcaagcaag aaaaagattg atagtatgac atttcatgaa ggtgattata tagcaaatga
     4081 aaaagataga acttttaaag ttgttggtat aattgaaaaa cctgggtttg aacgttataa
     4141 tggaattgca acagctatag cctactttaa tcctatgaat aattacaatg atacaattaa
     4201 tgtatcagta gcagtaagaa atcctaaaga tgtgtataag ataagtaata aaataagtaa
     4261 aaatatatat tcaaataaag ataaagaagc agtttctgat atgataaaat acaatgaaca
     4321 tttattgagg ttacaaggtg ctagtaagta ttcaaatata aataactcta taaagtctat
     4381 aattatagca gtaactattt tagtaatcat atgtacaatt gctacagtgt acaattcctt
     4441 tagtatttct ataaatgaac gaaaaaaaca gtttgggata ttaaattcta taggtgctac
     4501 atctagtcaa attaaaagat tagtttttat agaaggtatt ataattagtt taattggtat
     4561 accaatagga ctaatatctg gaacagtagc tatagaccta ttgtttaaaa ttataaataa
     4621 gtattttact gaatcggttg ttactaagat gagtttgcag atagtgtata atccaataat
     4681 aactattgtt agtataataa tagttttgct tacaatcttt atatcaattc tactacctgc
     4741 aatatcggct tctaatattt ctcaccttaa tgtgattaaa aatactggag agtataaagt
     4801 gggcaaaatc aaaagctcaa gacttattaa aatgattttt aaaactgaag gagtacttgc
     4861 atataaaaat attaggagaa atagaaaaaa atttataata actctttttt ctttaatggt
     4921 aagtataata atatttatat cttttagtgg ctttacatta cttttactaa gaggtgaaga
     4981 aattagaaat tctcaaagaa aatatgattt gtatttaact gcaaagaaca cagctaggtc
     5041 tgtagaggat actataagtg aactagaaga tattgatggg ataaaaaact ttgaattggc
     5101 aacaggacaa tatgtgtcta taagagtaag tgaaaataaa ataaataaat ccaaggaaga
     5161 tttaatcagg aaatattatc aaaaatataa gattggagat agttatgaat atgatttcag
     5221 taataatgaa ttaatttttc caggagattt tgcagtaaaa aacaaaataa ataacttagt
     5281 acagggctct tttaataaag agagagcaat tgaagaaaat ggtgttatat tagttagaaa
     5341 aagtgcattt gaagaaaatg ggaagaaagg tgtagtagaa cttaccaact ataaagtagg
     5401 agatacagta aattgtgaat atcttgatga aaatgcctta agtaaaaaag taaaggtcaa
     5461 gattcttgct ataactgacg aagaacgtct aggtcttggt tatcaaaata tggggttaca
     5521 atttataact tatgatgaag ttgctaaaaa tttaaattta aaattaagta aaagccttat
     5581 atttattgat tcaggaggaa gcattaagac taagaaggaa atagaagctt tggcaaataa
     5641 aaataatttt aacttttatg atgaaagtgc aagcaatgaa agtgaaaaac aagatttaaa
     5701 ggttattaaa atatttgtat atggttttat agtagtgata tctttagtga gtgttacaaa
     5761 tattctaaat acagtaagta caagtataaa tctaagaaaa agagaacttg cagtaataca
     5821 atctataggg gttactccta aaggatttag gaaaatgata tatttagaaa gttttatata
     5881 tggtatactg tcattgttgt ttggaatacc aattagtatt ggtataattc taattatgaa
     5941 taaacttatt tcaggtgtaa ttgaattttc tccgattata ccgtggacag ccatagttat
     6001 ctgtatagta agtgttttta taataacttt tatagc
//
LOCUS       DAEOSP010000202         6022 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.206, whole genome shotgun sequence.
ACCESSION   DAEOSP010000202 DAEOSP010000000
VERSION     DAEOSP010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6022
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.206"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..410
                     /locus_tag="KRH54_003096"
     CDS             <1..410
                     /locus_tag="KRH54_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4004 family protein"
                     /protein_id="HBG8580730.1"
                     /translation="IANMFMKKDINKEFDIDFILSKEVICNYTKEIFQNLYKKEQSIG
                     KKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKIYLFRKFGVPFVVG
                     CLDYKKVKFEKDVVKIIEVDLTKEINDISIKLS"
     gene            complement(710..1297)
                     /locus_tag="KRH54_003097"
     CDS             complement(710..1297)
                     /locus_tag="KRH54_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBG8580731.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(1383..1820)
                     /locus_tag="KRH54_003098"
     CDS             complement(1383..1820)
                     /locus_tag="KRH54_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8580732.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            1924..2922
                     /locus_tag="KRH54_003099"
     CDS             1924..2922
                     /locus_tag="KRH54_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBG8580733.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            complement(3042..3968)
                     /locus_tag="KRH54_003100"
     CDS             complement(3042..3968)
                     /locus_tag="KRH54_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBG8580734.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            4354..5958
                     /locus_tag="KRH54_003101"
     CDS             4354..5958
                     /locus_tag="KRH54_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8580735.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENHIRPVMIHAQTVREDQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
BASE COUNT         2244 a          753 c          943 g         2082 t
ORIGIN      
        1 atattgcaaa tatgtttatg aaaaaggata ttaataaaga atttgacatt gattttattc
       61 tcagtaaaga agttatatgt aattatacca aagaaatatt tcaaaattta tataaaaaag
      121 aacaaagcat aggtaaaaaa gaactgctta tattgagcat aatagagaag tttttagtta
      181 aatctataat aacttttgaa gagttaaaat tgatagtgaa tataatagaa gaaaatttta
      241 catcaatata caatgaaagt ggaaagattt atttgtttag aaagtttgga gttccattcg
      301 tagtaggatg tctggattat aaaaaagtaa agtttgaaaa agatgtagtt aagattattg
      361 aggttgactt gacaaaagaa ataaatgata taagtataaa gttatcatag tgtattatct
      421 tatataatgt attataataa tctacttaaa atacacttta tttaaatatt tagtttagag
      481 atgaggattt atgtgagtat gtaaaagtta aagatagatt taagtaagtt atagggatta
      541 gtaaacaata aaatgaacca tgatgaacta ctataaattg tatttaaact acattttcta
      601 cttatagtaa tatcaatggt tcattttatt gttattaaga taatacagta tttataaaaa
      661 tacttgatat aaatatagta aattgtttaa atacaatgat taccttagtt tattcaaata
      721 atgagttttt aaaatctaag aaatatcctt gtggatgaag acatacagga caagctttag
      781 gagcttcttt acctatatgt atatggccac aatgtgtaca tatccattgt atatcttcat
      841 ttcttctgaa taaagtacct tcttctaaat cagtagcata tctaccaaat ctatcaccat
      901 gaactttttc tatttcagct atattttcaa ataacttagc tatagattca aatccttctt
      961 cacgtgctgt ttttccaaag tttttatata catcatccca ctcttgaagc tcattatgtt
     1021 gtgcagcttt aaggtgttgt gctgtcttgt catataaatc tacagggtat gctcctgata
     1081 tacttatatt ttcaccagca aattctttta atttagaaaa gaattgtttt gcatgtgatt
     1141 tttcttggtc agcagtgtaa ttaaataaag actctattat atataatcct tctttttttg
     1201 ctacagatgc agaaatattg tatctatttc tagcttgact ctcacctgca aaagctctta
     1261 ataaattttc tttagtttta ctatctttta aattcatatt ctacctctct atcacaataa
     1321 atttttacaa tatttgattc tttttattta ttattccaat aggagatata attatgctca
     1381 atttagtcag catttttaaa attatcatta atttttttta aaattctaag aaattcttgt
     1441 ttttcatttt ctgtaatatt atggtatgcc gtctctttaa cttgtgttga gatgctatta
     1501 aaagtatctt ctataagcag tgcctttttt gtaagcttta tatacgtgat acgtttgtca
     1561 tttgtacatt tttgtctttc taaatatcct aggtttataa gtttattaac caacactgta
     1621 acagtggatt tatcttttcc tattttagct gcaatttctt tcattgtaag tattccatta
     1681 ttttcataaa gtgcagttaa aatatttcca tgagtaggta ttaactcacc taaaccattt
     1741 ttactgagtt tattttcaat gaatttaatc atactagcct ttgttttact tatgaaatgt
     1801 acaatatatt tatcttccat aaattattct ccaatcttta atctatttaa attagtttga
     1861 cataaaacta tttataaact ataattagtt taacataaaa ctaaatgtat tgggaggaaa
     1921 tttatgaata aaaaatttaa atcactatat attacagaag aagaaggaaa ttttagaaga
     1981 aatatagttg aaagagaagt ggatacattg ccagaaggcg aagttttaat aaaagtaaaa
     2041 tattcttctt taaattacaa ggatgcactt tctgcaaatg gtaataaggg tgttacgaga
     2101 aagtatcctc atacaccagg aattgatgca tctggtatag tagaatctag tagttgtgat
     2161 gattttaaaa ttggagaaga agttttagta acaggttatg acttgggaat gaatacttct
     2221 ggtggttttt cagaatacat aagagttcct tctagttggg tagttaaact tcctaaaaat
     2281 ctttcactaa aagaagcaat ggattatggt acagcagggt ttacagcagc tttatctgtg
     2341 tataaattta tagattctgt agacaagaat atgggagatg tattagtcac tggtggtact
     2401 ggaggagttg ctgtaattgc aactaaaata ttgattaaat taggttattc agttgtagtt
     2461 tcaacaggaa aattagaaga acaaaaagaa gtattactaa acttaggtgt taaagatgtt
     2521 atacataggt cagaagtgga tgataactca ggaagacctc tgcttagacc aagatgggct
     2581 ggagtaatag atacagtagg aggaaatact ctagcaacag caataaagac aactaattat
     2641 tgtggagcag taacaacttg tggtaatgca ggaggagttg attttacaag ttcagtatat
     2701 ccatttatct taaagggaat tactttatat ggtatagatt ctgtggattg cccaatgaac
     2761 ttaagattaa aaatatggga tttattatca aatgaatgga aattagatga tttagatgat
     2821 atggtaaatg tagtatcatt ggaagagtta aatgatagta tagattcaat aatggcagga
     2881 aaacatgttg gaagaacagt agttgattta gaaaaaatat aggtgaataa atttataatt
     2941 aggattatag gttttaagca aaaatgagat acttgaaatt tacttatgag tatctcattt
     3001 ttttatgtaa aaagatttat gtagatgttt gtgtaaaaag attattcatg aacaattaat
     3061 tcaaatacat ctggtcttga atagtgacct tctgaatcaa aatctagtct actaagtact
     3121 attttttcta ggtctaaatc agctattagc atttcttctt tattgaaaat aggacctgct
     3181 acatattttc caaaagggtc aactatacaa cttcctcctg gacacataat ttctggttct
     3241 gcatctagct ctttatagta gttaaggtca gttggataca tatttttttc aacatattga
     3301 ttgcatccaa ttacaaaaca tcttccttct aaagcaatat gcttcatagt tgcttgccac
     3361 tcttctcttg aatctgcagt aggggctatg taaagtttta cacccttagc ataaagagct
     3421 gttctagcta atggcatata attttcccaa caaatcaacg aacccatttt tccatatgga
     3481 gtatcaacta ctgtaagagt acttccatct ccttctcccc aaatacatct ttctgttcct
     3541 gtaggtttaa gttttctgtg tttaccaatt agcttacctt ctggagagaa aaataagttt
     3601 gtgcaataaa gggtacaatt tatatcatta ccatctcttt ctgttatacc cattgataga
     3661 tatactcctg cttcttgtgc tgcttttcct aataaatctg ttgtagcact aggaactggt
     3721 acagaattat catagtatct tttccaatct tctctacctt ccattgttct acttccaaca
     3781 acaaatccaa atgaaagacc tcttggatat gcaggtatga atgcttcagg gaaaactacg
     3841 atatttgccc cttttctacc agcttcttta attaatccta atgctttttc aacagttttt
     3901 tctaaatcca ttatgactgg tgacgcctgg acaacagcaa ttcttacatt tttattttcc
     3961 ttgctcataa aaaacctcct aaaaaatatt taaatttttt gcattgaata gattacatga
     4021 gatgttattt gagaactaag atacttatat tgtgaataat aatgtaactg tatatttaaa
     4081 ataatacatc atgatttaaa aaattacaat tgtacagata taattgagtt tagatattta
     4141 aatttaacaa cttataatat tttttcaatt agtataaagc ctattaattt gtcttattta
     4201 caatgaatag tatttacttc ttggcaaaca ataattaaaa ataaaacttt tatatgatta
     4261 aactctaaag aaaaagttta taaagaagat aatataatta agcagaaaat aatgaaaaaa
     4321 atttattaaa gtagacaacg taaggagaat tttatgaaag aaaaaattta ttacaatgga
     4381 aatatcatca caatggaaga ttcaatatgt ggagatgcta ttcttataaa agacaaaata
     4441 ataaaaaaga taggaacaaa agaagaagta tttgctttga aaaataaaga tacagaaatt
     4501 atagatttac aaggtaagac attgatgcca tcttttatag attcacatag tcacttgata
     4561 gcttttgcta ctactctaaa gctagttcct ttagaagatg caaccagttt taaagacata
     4621 gtaaaaaaaa tacaggattt caaagagtcg aataatataa aaaaaggaga ctggataatt
     4681 ggattttcat atgataataa ttttttagaa gaaaataagc atccagataa gagtgtactc
     4741 gatagtgcat cttcagaaaa tccaatacta atttcacatg cttctggtca tatgggagtt
     4801 gcaaatacat tggggttaaa acagcttggt gttaccaatg aaacacgtga cccagaaggt
     4861 ggacatatag gtagagtaga aggcagtgaa gaaccaaatg gatatttaga agaaaatgca
     4921 ttttttaatg tagcatctaa aataaaacaa ccatcatcaa atgagatttt taattcaatt
     4981 gaaaaagcac agaacatata tctaagttat ggtattacaa cagctcaaga aggtttaatg
     5041 gaagaaaatc aatttaacat tttaaaagca atggcaaatc aaaataaatt aaaaatggat
     5101 gttgtaggat atgtaaattt gaaaaaaagt aagtctgtag cagataataa tagggagttt
     5161 ataaaaaaat atataaatag atttaaaata ggaggttata agattttctt agatggttct
     5221 ccacaaggta agacagcatg gttgagtaga ccatatgaaa attcagatga tggttattgt
     5281 ggatatccta tatacaaaga tgaagaagta gaaaagttta tagatatttc actaaaagaa
     5341 aagatgcaac tcttaactca ttgtaatgga gatgcagcag cagaccagtt gatagatgcg
     5401 tttgagaaag ttttaaactt aaaagagcaa agtagtgaaa atcatataag accagttatg
     5461 atacatgctc aaacagtaag agaagaccag attgatgata tgaaagtaat aaatatgata
     5521 ccttcatact ttgtagctca cacatattat tggggtgata ttcatatcaa aaaccttgga
     5581 gaagatagag cgtttaagat tagcccactt aaaacaacta tagaaaaggg attaatttac
     5641 actcttcatc aggatacacc agtaatagca cctaatatgc tggaaacagt atggtgtgct
     5701 gtcaatagaa ttacaaaaaa aggtatacaa ataggagaaa atgaaaagat aagcccatta
     5761 gatgctttaa agggtgttac tataaatgca gcatatcaat attttgaaga agataaaaaa
     5821 ggttcaatta aagaagggaa attagctaat ttgataatat tagatgaaaa cccacttaca
     5881 atcgacccta tgaaaataaa agatattaaa gtattgcaga ctataagaga aggagaagta
     5941 ttgtattctt tgaagtagat gaaagttaga tattttataa tctataaaaa atagttttat
     6001 ataataagtt aggtagaatt aa
//
LOCUS       DAEOSP010000203         5996 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.207, whole genome shotgun sequence.
ACCESSION   DAEOSP010000203 DAEOSP010000000
VERSION     DAEOSP010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.207"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            159..971
                     /gene="modA"
                     /locus_tag="KRH54_003102"
     CDS             159..971
                     /gene="modA"
                     /locus_tag="KRH54_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG8580736.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            981..1772
                     /locus_tag="KRH54_003103"
     CDS             981..1772
                     /locus_tag="KRH54_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8580737.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQIFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            1774..2469
                     /locus_tag="KRH54_003104"
     CDS             1774..2469
                     /locus_tag="KRH54_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8580738.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMSVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            2456..3187
                     /locus_tag="KRH54_003105"
     CDS             2456..3187
                     /locus_tag="KRH54_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580739.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLERLCFCSK"
     gene            3501..4301
                     /locus_tag="KRH54_003106"
     CDS             3501..4301
                     /locus_tag="KRH54_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBG8580740.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            4841..5557
                     /locus_tag="KRH54_003107"
     CDS             4841..5557
                     /locus_tag="KRH54_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8580741.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
BASE COUNT         2328 a          727 c          849 g         2092 t
ORIGIN      
        1 actacctatg gaaatgacat tataaataga ttttttctta actatacata acaaaacata
       61 acaaaacatt ataaaacata acaaaaactt agacatcgtt ttcttagtat gatatactaa
      121 aaaagaattt acactaattt actaaggggg aatatcttat gtttaaaaaa ctttcagtac
      181 tagctttagc tatgattatt tcattaagta ttactgcatg taattctaaa gaaaacaaag
      241 ctaatgatga atctaatact aaagtgacaa cagctgatga ttcaaaagaa aaagagactc
      301 tttttgtcta ttcaggggca ggtttaaaga aaccaatgga tgaaatagca aaaaaatttg
      361 agaaagaaaa taatgtcaaa gttgaatata gctatgcagg ttctgcacag ctaatagctc
      421 aaattgagac aagtcacaaa ggagacgtat ttattgttgg ttctgaacca atttattcaa
      481 aggcctttga aaaagaactt gtaggtgaac ataaaacggt tgcacatcat acacctgcaa
      541 tagttgtacc taaaggaaat ccagctaata ttcaaaaatt ggaagactta aataaagatg
      601 gagttaaact tattttggga gacaaagagt ctaatgctat aggtaagact acacaaaaaa
      661 tacttgaaaa aaataattta aaatctataa atgataatgt tgtctctaca gctgcaactg
      721 taaatgaaat gattgtacag ttaacatcaa gtaaagctga tgctacaata gccacaaaag
      781 attcggtctt tggtaatgaa gatgtagaag ttatagaaat tgcccaagat aaaaatatag
      841 accaacttat atcagcagga atcgtaaact atagtgataa aaaagattta gctaataaat
      901 ttataaatta tatagagtca gacgaagcta aacagatatt taaaaaatat ggttttgaac
      961 ctgttaagta gtgattaaac ttgaaaaata aattttttac tactctactt actggatttt
     1021 ttgctatagt ctttttgttt attgtatcac cacttcttat gttaatcatt aaagggataa
     1081 gttatgttcc tatatgctta aaatcagtgg aagtacagta tgcaataaca cttagtatta
     1141 aaacatcttt aatatctact atagtatgcc ttttattagc tattccagta gcatattttt
     1201 tacatattac taaattacca tttaaaaaac tgattataca gataataaat ctccctatgt
     1261 ctcttccaca cttagtatca ggaattgctc ttttactttt gtttggaaga atggggattg
     1321 gagattccat ttataagatt tttaagcttg attttatatt tacaaagcaa ggtatagttt
     1381 tagcacaaat atttgtaaat cttccactta ctattaaaat acttcacact tcattaaatg
     1441 aaagtaatga aaagatgata tttgttgcac gtacccttgg atgtaatagt tgggaagcat
     1501 ttagatttat aattcttcca aatctaaaaa ctggaatcat atctgcaact gtaatgactt
     1561 ggtcaagagc acttggtgaa tttggtgcag tagctatgat agcaggttct acacgtatga
     1621 aaactgaaat tattcctaca tctatatatt taaatatgtc tactggtgat atagacattg
     1681 caatcggaat agctgtaatt ttgatattta tctcacttac atgcttgatg ctttttgaga
     1741 tattttttaa tagagaggtt gataagaatt aatatgttaa aaataaaaaa tttatctctt
     1801 gatttaccta attttaaatt acaagaaaat agtttaaata ttaataatca tgaatacttt
     1861 gttttattag gagctagtgg gtcaggtaaa actctattcc tagaaacatt agcaggaaga
     1921 tatccatctg ctaaaggaaa tgtatcatac aaagaaaatt taatatcaaa cttacctcca
     1981 gaaaatagaa atataggatt tgtatatcaa aattttgaat tatttccaaa tatgagtgtt
     2041 gaagataata taaggtttcc acttaaatta agaaaaatac ctatagatga acagattctt
     2101 aaggctaacc acttgtctaa attaatgcag atagaagata taaaaaatag gtatcctaaa
     2161 aatttaagtg gaggcgaaaa gcaacgagtt gcttttgcac gtgccttaat agctgaacct
     2221 gatatattac ttttagatga acctatgagt tcattggatt atattacaaa aaaacatata
     2281 agcaaaattt tgaaggatgt ctacataaaa tacaaaccaa cagtgattca tgttactcat
     2341 gatatttctg aagcaatgtt ttttgctcat aagataggaa taatgaacaa tggaaaaata
     2401 aatcatattt ttgatttaaa tgaagaaata aaaaataaag gagaatcttt tttctatgaa
     2461 tatatttgat attttgagag attatctatt agtacaagtc gataaatata atttaaatat
     2521 ggatatgata agtattgtgt ccaaatctct atcttcaaaa gaagcaattg gaaatactaa
     2581 gcgtaaagat tttccaatta tagttggtaa agaaataatg cttgaagctg actttaaagg
     2641 agctaaagga caagcattta cttctactcc atctacattt gaaggttctt taaaagacat
     2701 attatcatta gatttacatg acaatcctca tgatagaagt ctttttatag catcattaaa
     2761 tgcagttatg aaatacttag gcaagacgga tagaaccatt cattgtaaaa ataacgagcc
     2821 agaggtatgt gcaaaaaaat tccctgagtt tataaaaatg gaatttggta atccaaaagt
     2881 agcaattata ggatatcaac cagccataat tgataatatt aaggactttt tcgaaacaag
     2941 agtattagat ttaaatcctg aatttgtgga tactattcaa tataatgtta agattgaaga
     3001 tggtatacga gattatgaag atgtaatatc ttgggctgat ttagtgattt gcacaggaag
     3061 tacattatgt aataactcaa ttataaattt tctatcactt aacaaaccag tttactatta
     3121 tggaactacg atagctggtg catcaaatat tttaggttta gaaagactct gtttttgtag
     3181 taaataagca atcatatcat caaggagtac acaaatatat atgtgtactc tttaaattta
     3241 aaaagttatt acttttattc taagtactaa agtaaaattc tattaaaatg aatcccgaat
     3301 aaatgtacaa tccctttaaa tctcaattcc tatcaatcat taatattttg attagtctta
     3361 tacataaatg ttttctttat tttaaactaa taaaaaaatt ttattccatt aatctactct
     3421 atatttttca gcatttaaga aataattttt agtactttgt ttgataatat aactaagaca
     3481 acttttcagg agatgattaa atgacattta agaataaaaa atttactata aataaatata
     3541 aaattctatc tattattcta gttttatgtt tgattttaac cgcttttagt agctatgtga
     3601 attatagtca taaaaaattt aaagattatg ttgtcttcta tgttcagcat caagatgatg
     3661 aagtgttgtg ggctgggagt gctattgtta atgctataaa agaaagagga aaaaatcatg
     3721 tatttgtggt tttagtatct actggatgtg gtatatccgt ctttgataaa tataaaaaat
     3781 atgaaaacct aacaaatata caaaaatcag aatatagaaa tagagaattt ttagcttctc
     3841 ttaatagcct tggtgtaata agggaaaata taatattatt accagaaaca aatacaaatg
     3901 gtaatactga cttcaattac atggaaaaaa ttgctcttac atttgaagaa aaatttaaaa
     3961 atgtaactca tatagcacat acttataagt tagatgacca tttacaacat ttaaaaaatg
     4021 gttctgttat ccaaaattta tataatgctg gtaaaataaa agatgctaaa tattttgtaa
     4081 aacctaaatt tgctgataaa ataactttta ataataagat agtttataaa gctcattcta
     4141 ctgaagacta tgaaaaggtt aaaaatgcct gtcaacaata caaaattgtt gatgaattag
     4201 aatatagaga aggtattggc tataagtctg accataaatc atttgataag ttattaaata
     4261 ataaagatgt tcctgctata cttcatacac ctaatttata agaaatattt attcttttaa
     4321 tttaagttat aaaaaggaaa ttatttgaga ttttgtgata tagcactttt tgtgctatac
     4381 tactttttct catacaacta ttacccatat ctaatttcat catctcttaa aatatataaa
     4441 gatatcatca ttaaaaccta taagccttat taaatgcttt atacatacct acattaaact
     4501 acctgtttat attaaactac attaaaataa atatttgtta taaattctta ataattatga
     4561 aactcatttg ttatttatat ttgttaatat atgtttaaac ctaaaaaatt aataaaccaa
     4621 aataacataa gaagtgtgat agattaccct agatattttc ataaactttt accttcagat
     4681 taatatgggc gtctatactt ttaaatttac tatatgcatt gacaatatat taaaagtact
     4741 agtttttatt tcataatgaa cgggcttact gttttctaat acctcttttt ggtataaaat
     4801 aaaaattggt taaaaataaa atttcaaggg ggttaataag ttgaatcaaa atatattggt
     4861 tgtagatgat gatagagaga tagttgatgc tatagagttc tatttgagac ctgataactt
     4921 taatgtttta aaggcatatg atggacttga agcattggac atactaatcg aaaatgatat
     4981 taagttaata ataatggatg taatgatgcc caatttagat gggcttaaaa ctacacttaa
     5041 aataagagaa aaaaggaata taccaataat tctcttatct gcaaaatcag aagatatgga
     5101 caaaattata ggtcttaata tgggtgctga tgattatata actaaaccat ttaatccact
     5161 agaacttact gctcgagtaa aatctcaact tagaaggtat ataaatttag gtgattttag
     5221 taatacaaat aactcaaatg atactaatta tattctaaaa agtggtggcc tagagctaaa
     5281 tacagataca aaaatagttt ctttagatgg tgaggaaatt aaaatgacac cacttgaata
     5341 taaaatactt acacttctta tgtcacgaaa aggtaaaatt ttctcttctc atgagattta
     5401 tgaaaatgtc tggaatgagg atgcttatag ttgtgagaga actattgctg ttcatattag
     5461 aagaattaga gaaaagattg aaattaatcc aaaagaacca aaatatttaa aggtggtgtg
     5521 gggcattggt tacaaaattg aaaaacttaa ttattaatga ttctaaagca aaatttattt
     5581 tattattatt actgtttcta ttagtttcta tattatttga agcagtttat gatttgaata
     5641 ataacttttc ttataatatt aattgttatt ataaagagtt tgctctccct tatttttatt
     5701 ttttcggtac tcctgtacat ttgtcaaaag tagaattaga gtttgtctgc ataggtatta
     5761 tattatcaag ttttttattg actatactat gttttacatt gtataaaaaa aataaacaga
     5821 ctaaagtcaa attcttagag aatgttataa gccttttttc taaggtaaaa atagaactta
     5881 cacttatatt aactttgata ctattaacct taaggtttca tgcatttaca agtaataaaa
     5941 tgttttacaa cactaattta ataatctaca attttataat tatatctata gtctat
//
LOCUS       DAEOSP010000204         5890 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.208, whole genome shotgun sequence.
ACCESSION   DAEOSP010000204 DAEOSP010000000
VERSION     DAEOSP010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.208"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            49..615
                     /locus_tag="KRH54_003108"
     CDS             49..615
                     /locus_tag="KRH54_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG8580742.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            655..1236
                     /locus_tag="KRH54_003109"
     CDS             655..1236
                     /locus_tag="KRH54_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBG8580743.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(1293..2210)
                     /locus_tag="KRH54_003110"
     CDS             complement(1293..2210)
                     /locus_tag="KRH54_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBG8580744.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            2340..3971
                     /locus_tag="KRH54_003111"
     CDS             2340..3971
                     /locus_tag="KRH54_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBG8580745.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            3974..4921
                     /locus_tag="KRH54_003112"
     CDS             3974..4921
                     /locus_tag="KRH54_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBG8580746.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5206..5469)
                     /locus_tag="KRH54_003113"
     CDS             complement(5206..5469)
                     /locus_tag="KRH54_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBG8580747.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(5568..5813)
                     /locus_tag="KRH54_003114"
     CDS             complement(5568..5813)
                     /locus_tag="KRH54_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580748.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVINKVKTG"
BASE COUNT         2118 a          808 c          974 g         1990 t
ORIGIN      
        1 tacttgatac catcaataaa actaaaaata taaaaaggag aacttaaaat gagtgttagt
       61 ttacaaaaag gacaaagagt tagcttagta aaagataata atcctgtaaa aaatctagta
      121 gtaggcttag gatgggatat gaataaattg ggtaaaaaaa attatgacct agatgcattt
      181 gcaatagcat tgactaacca agataaaatg aatgttagag gtgacttggt ttattttgga
      241 aatcttaaac acccttctaa agctattata cacactggag ataacttaac tggaaaaggt
      301 gaaggtgatg atgaacaaat aattgttaat ttagaagaca tacctgaata tgtacataaa
      361 atagtttttg gagtttctat atacaaagct aaaaaaagag accaggattt tggtcaaatg
      421 aacaactctt ttataagatt aattgattca aattccaagc aagaattatt taaatataat
      481 cttcaagaag acttttctgg taaagtaaca gttttggcag gagaaattta tcgtagaaat
      541 gaagaatgga aatttaatgc tttaggaatt ggtcaaaatg aagagcttcg tgaattaatt
      601 aatacatata agtaataatt acaataaaaa atttataatg aggagttggt ttaaatgggt
      661 ataactttag caaaaggaca aaaagtaagt ttaacaaaaa gtaaccctgg tctaaaaaaa
      721 gtaatagtag gactaggatg ggatattaat aaatatgatg gtggctttga tttcgattta
      781 gatgcctctg catttttaac tggaactgat ggaaaagtaa cgaatgatgg agactttata
      841 ttctataata atttaaaaca cgcttctgga gctgttgaat atatgggtga taatagaaca
      901 ggtgttggtg atggtgatga tgaacaaata aatgtagact tatctaaaat acctcaaaat
      961 attgctaaaa tatcatttag tgttactata aacgaagcaa ttacaagaag acaaaatttc
     1021 ggacaagttg aaaattcata tataagaata tataatgaag aaactaatga ggaacttatt
     1081 aaatatgaat tgggtgaaga ttttagcata gaaacagcaa tagttgtggc agaactatat
     1141 agacataatg gagaatggaa atttaatgct ttaggttctg gatttgagga tggacttgca
     1201 ggattgtgta aaaattttgg cgttaatata ggctaatata tctaaataaa aataagagta
     1261 tattgatata ctcttatttt tatttagata tattataaac tgtttacact tctactcttc
     1321 tttctcgctt gttttttccc atattctact gaaatatcaa aaaatgctga aatattttct
     1381 gtaaaaaact tgcttctatg atatgcaata tcaaatgtcc tatgtaaatt aataccttca
     1441 atatcacaag cccacaaaag atgctttttt aaataatcct caataagtaa ttcggatata
     1501 actgcaatgc caaaattatc tacaactgct cttaatatag cttcagagtt ataacatgac
     1561 catctaatat tcattgggat ttttaattct tttaattgct tttctaattg tgctcttgta
     1621 ccacttccta attcacgcat aattaatggt tgatttgaaa gttctgaaac ttttatagag
     1681 tctcttttat aaaactcatg cttgtgtgaa catataagta caagattatc attaataatg
     1741 ctttttgtaa caatatctgg gtgcttaatt tttccttcta caatcgcaat atctaattca
     1801 ctattaagta atttttttgc aatcagtctt gtatcttcta caaatacttc aggttctata
     1861 agaggattta ccttgtataa ttctaacatt attggactta ttacacatgt accaacagtt
     1921 actgttgccc caactcttat acggatattt tttgcacaat gcttcataca atcattcatt
     1981 tcttcattta cagctaaaag atgcctccca tatccaagaa gttttttccc tgcctcagtt
     2041 aaatataatt tttttgaaat tctatcaaat agcttaacac catactcttt ttctatttct
     2101 gaaatagctt ggctaactga tgactgggta atatacatat ttcttgctac ttcactcatt
     2161 ttaccacatt cagcaacctt aacaaagatt tcaagactac gaattgtcat attatcacaa
     2221 ccttagcaaa cattagtaaa tgcttatgat tttattaata ttttagtatt ttacaaatat
     2281 attgtcaaga agtataattt tattatagag ttataaagta gattggagtg tgatatattg
     2341 tgaaagtatt aattataggt ggagttgctg ctggaacaaa aactgctgca aaaataaaac
     2401 gtgaaatggg tgataattgc tctgttacaa tactaaataa aggtgatgat atttcttatg
     2461 caggttgtgg tcttccatat tacgttggta agataattga agataaatca tctttaatcg
     2521 taaatacacc tgaaagtttt tctaaactaa caggagcaga agttcgttgt ggagttgaag
     2581 ttacatctat tgatagaaat gcaaaacaag taactttgaa aacgctttca acaggagaaa
     2641 aagaaaatct aagctatgac aaacttgtaa ttgctacagg ggcagaccca ataaaaccac
     2701 caatacctgg aatagattta cctggagtat tctttatgcg tactccaaat gatgctataa
     2761 aactaagaga ttctattgaa aacggtatta agagagctgt tgttattggt ggaggtttca
     2821 ttggtcttga aattgctgaa aatctttctg caatgggtat acgtgtaagt gttgttgata
     2881 tggctgaaca tgttatgcct ggatttgata ctgattttgc tgaatacgtt gaaaatcata
     2941 tggctgataa aggcataatg atttttacag gtgaccaggt tgtaggtatc gaaggtgaaa
     3001 ataaagttga aaaacttcgt actaaaaata gagctattaa aacagattta gtggttatgt
     3061 cagttggaat ccgtcctaac acaggttttc taaaagacac aggtttagaa tttgccccta
     3121 ataatacaat tattgcagat gaatatatgc ttacaaatga caaagatata tacgtagttg
     3181 gagactgtgc ttttgtaaaa aattctttaa caaataaacc tacatggtca cctatggggt
     3241 catctgcaaa tcatgagggt agaatttgcg cacaaaatat atctggaaaa tctaaaactt
     3301 acaatggtgt tcttggaaca acagtagtaa aattacctga gctcaatgca ggaaaaacag
     3361 gtctaagtaa agaaatagct gaaaaagaag gttataatgt ttgctctgta accatagcta
     3421 ctgatgataa agctcattat tatccaggtg catctaattt tattataaga atggttgctg
     3481 aaaaaaatac tagaaaatta ttaggtgttc aagttatggg acctggagcg gttgataaaa
     3541 tagtagatat cgctgctact gcaataactt taggcgctga cctttattca ttagaaagta
     3601 tggaccttgc atatgcacca cctttttcaa cagctattca cccttttgta gtggcagtaa
     3661 atgttttaca aaataaactt aatggagaac ttgatagtgt tttacttgac gaaattgagg
     3721 attttgacag ttggactaaa cttgatgttt caaaagctcc ttctattcct agtttacgtt
     3781 atattcctgt tggtgaaatt aatggagaaa ttgaaggcct tgctaaggat gaaaaaattc
     3841 ttcttatatg tgctaaaggt aaaaatagct atatggcgca aaatcgctta agacgctttg
     3901 gctatacaaa tacgaaagtt cttgaaggtg gaatactatt ttatccaaat cttaaggtaa
     3961 tggaggagta aatatgagta atgttacagc agaacaaaaa aaggaagtaa aaggtagagg
     4021 ttttctacca agcagggatg gagaacattt tgcagcacgt ataataactg taaatggtgt
     4081 aataaatgca tcacaagcaa aaaaaattgc agaagctgct gaaaaattcg gaaacggtca
     4141 gttagcattt actactagac taacagtaga attacctggt gtaaaatttg aagatattga
     4201 agctctaagt gaatttattg ctagcgaaaa tctaataaca ggtggaactg gatcaagggt
     4261 tcgtcctgtt gtggcttgta aaggtactgt ttgtgttcat ggtctagctg atacacaggc
     4321 acttgctgct gaaatacacg aagaatttta caaaaattgg tatgatgtaa aactacctca
     4381 taagttcaaa attggtgttg gtggatgccc taataactgt attaaacctg acctaaacga
     4441 tcttggaatt gttggtcaac gtgtacctga ctatgattca gagctttgtg ttggttgtaa
     4501 aaaatgtgct gttgtagagg tctgtcctgt taaagcagct aaattaacag ataaaggtaa
     4561 acttgaaata gatagtaatc tatgtaataa ctgtggtaag tgtatagaaa gttgtaattt
     4621 tgatagtatt gaagaaaaag aatctggtta taaagtctat ataggaggga aatggggaaa
     4681 atcagttcgt cctggaacac agattgatag attattttca aaagaggaac ttatgacttt
     4741 aattgaaaaa gcaattcttt tatttagaga acagggtaac acaggtgaac gttttggaat
     4801 aactatcgat agaatcggtg ctgataaatt tatagaaatg cttttatcag atgaagtttt
     4861 ggaaagaaaa gaagagatac tagaggctct tttacacctt acaggtggag ctgtatgcta
     4921 acaataaata ttttaattgt acagataacc tatggaaaaa attttagtta agaataatta
     4981 aaattctata tatgatatat gataaatgag gctatctcaa aacacagata cgcctctttt
     5041 tcattgttga aatgataaag tatatttgag ttcatataaa aaagagtgtc tcgtgaatta
     5101 taaaattcat tttgaggcac ccacattatt ttataataat tgcttattaa ttttatttta
     5161 actatataaa aagtacaaaa agtaagaata tccaatattc taaaattaat tttgaattgt
     5221 attgattgaa aagtttacta ttccactatt tccatcttga tttactacta gataccatgt
     5281 tccagtatta ggaactctca ttctacatgg atttttcttt gcaaatccac catagtaatc
     5341 ataatttctt tgatttttaa atttcacata atttatatgg tctaacaata ttacattggc
     5401 ttgtttctct aaatttacct ctacaatatc cccttttttt aagttcatct tactataact
     5461 atattccatt gaattactcc tccagtatcg aataatcttt gttatttact catctcaaat
     5521 atgtaaaaat tcatttaaaa tattacaaat actatataat tagactatta tccagtttta
     5581 accttattta ttacaatcca aaggttatta aaaatattat tggcaacaac ttctccatcc
     5641 catacaactc tttcttcttt attttctaat aaagcatagt acccagagtc agaactttca
     5701 tctttataga ctgtaacata aaactcttca tcactatatt caggtatatt tttaaagtct
     5761 tcagatattt tacatgttaa tatataacct tctaattctt catctataaa caaagcaatt
     5821 cctccaatct ctatattttt actaattata ccatattatg tcataaactt tttattttat
     5881 tacaaatttc
//
LOCUS       DAEOSP010000205         5784 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.209, whole genome shotgun sequence.
ACCESSION   DAEOSP010000205 DAEOSP010000000
VERSION     DAEOSP010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.209"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(292..2124)
                     /locus_tag="KRH54_003115"
     CDS             complement(292..2124)
                     /locus_tag="KRH54_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580749.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(2117..4363)
                     /locus_tag="KRH54_003116"
     CDS             complement(2117..4363)
                     /locus_tag="KRH54_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580750.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(4431..4892)
                     /locus_tag="KRH54_003117"
     CDS             complement(4431..4892)
                     /locus_tag="KRH54_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8580751.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(5197..5763)
                     /locus_tag="KRH54_003118"
     CDS             complement(5197..5763)
                     /locus_tag="KRH54_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBG8580752.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         1845 a         1079 c          632 g         2228 t
ORIGIN      
        1 aattaaattc tatttataat aaaatttaaa gaataaaatt aaatatacat tttgataatc
       61 acctccaaca ttttccttac tacatattat aataaaataa acactttgta aagacataaa
      121 attatgtggc tatctcaaaa tatagagaat cctctttttc attgttaaaa tgataaaaat
      181 acactttatt tcatacacaa aagagtgcct tatgaattaa aaagttcatt ttaaggcacc
      241 ctcatttatt ttatcttatt aattacatgt ctatgctaag atagtttttt gttacattgc
      301 ttcatcttct tcaaattgag aattgtaaag attggcatag aatccgcctt tctttagaag
      361 ttcctcatga tttccttgtt caactatatc tccttcattc attacaagaa tcatatcagc
      421 atcacgtata gttgataatc tgtgggctat aacaaaactt gttctacctt ccataagatt
      481 atccatagct ttttgaatca atacttctgt tctagtatcg acagaacttg tagcttcatc
      541 taatattaaa attttaggat cagctaaaat agcacgagct atagtcaata attgtttttg
      601 accttgagaa acattacttg cttcttcatt taatttcatc ttatatccat ctggtaatgt
      661 cttaataaat ctatgaacat gtgcagattt agcagcctct ataacttctt catctgtagc
      721 attcaactta ccatatctta tattttccat aattgaacca ctaaataacc atgtatcttg
      781 aagaaccata ccaaacattt ctctaagctc actacgatta aaatctttaa tattatgacc
      841 atcaatcagt attgaaccgc tattaacatc ataaaatctc attaacagtt taactattgt
      901 agttttacct gctcctgtag gtccaacaat agcaactttt tgaccaggtt taacatttac
      961 tgagaaatca tttattatag tcttgtttgg gttatatcca aagttgacat tttcaaaatc
     1021 aattttacct tctaaaccat ctattgaaac tgcattttca acaatttgga cttcttcttc
     1081 ctcttctaaa aactcaaata ctctttcaga agctgctgct gttgattgca ataaattggc
     1141 aacctgtgca atctgagtca ttggctgtgt aaagtttctt acatattgta taaatgactg
     1201 tatgtcacca acctcaattg ttttcttaat tgcaagccaa cctcctaaga ttgataccat
     1261 tacatatccc aagtttccaa taaacatcat aagtggttgc attatacctg ataaaaattg
     1321 agatttccac gctgaattat acaggctatc atttagttta tcaaactctt caattacttc
     1381 atcctcacga ttaaatgctt ttactattgt ttggccactg taagtttcct caacttgacc
     1441 atttacattt cctaaatatt cttgttgata tctaaagtat ttttgtgatt tctttattat
     1501 tcttgaaatt acaaacattg atattggtaa tattaaaact gccactaatg tcataattcc
     1561 acttatagat aacatcatta tcaaaacacc tatcatagtt gttactgatg ttataagctg
     1621 tgtcatactt tgatttaaac tttgatttaa agtatctata tcattcgtaa ttctcgataa
     1681 tatttcacca tgcgtcttag tatcaaaata cttcattggc aatctatcca attttgctga
     1741 aatctctttt cttaaattat aggatacttt ttgagaaatt ccactcatta taaatccttg
     1801 tataaaagag aacagtgcac ttatcaaata aaggaaaagt aaaaaggtta agatttttcc
     1861 aattgcatta aaatctatac ctacgtttcc tccagaaact ttactcacta gcccttcaaa
     1921 tatctttgta gttgcctttc ctaaaatttt tggacctact atagaaaacg acgcactacc
     1981 aatagcaaat acaattacta gaattattga tattttaaat ttagataaat atgcaattag
     2041 ttttcgcata gtccctttaa agtcatttgc tttttgtcct gctcccatag attttcccat
     2101 aggacctctt cccattctac tcattagcta gctcctcctt tgaaagttgt gataaagcta
     2161 tttgctcata aacttcgcaa ttttttagaa gctctttatg tgttcctatt ccaactattt
     2221 cgccctcatt taaaacaatt atctgttctg catttaagat agtactaatt ctttgtgcaa
     2281 ctattagaac tgtactttca gcagtttttg attttaaagc cttacgaaga gctgcatcag
     2341 tttttaagtc aagtgctgaa aaactatcat caaatatata aacttctgga ttttttgcta
     2401 ttgctcttgc aatagaaagt ctttgttttt gacctccaga tacatttgtt ccaccttgag
     2461 aaatttcagt tttaaaagct tccggtttag aatctataaa ttctgtagct tgagctattt
     2521 cagctgctgt tctcatttct tcatctgttg cgtcttttct accatatttt aagttggatt
     2581 ctatagtacc agaaaacaat actccctttt gaggtacata tccaattttt tcccttaaat
     2641 catgttgaga aacatttttt atattaactc caccaagtaa tatttctcct tcagtaacat
     2701 caaagaaacg aggaattaaa tttataagag ttgacttacc actaccagta cttcctataa
     2761 aggcagttgt ttctcctggc tttgctgtaa aagatatatt tgttaatata ggttcttctg
     2821 cgtctggata catgaacgaa acatttttaa attcaacaag acccttttta tcttccttaa
     2881 actcttgtgc ctctgatttt tctgggtctt ttatgcttat atctgacact aatacttcat
     2941 ctatacgtgt agctgaaacg gatgctcttg gaagcataat agataccatt gatatcatta
     3001 agaatgacat tattatttgc attgtatatt gtataaatgc catcatatct ccaacttgca
     3061 ttgctccaga atcaacactg tatgcacctc tccaaactat aagaactgtg ataaggttca
     3121 ttataagcat catagcaggc atcatacatg tcataactct atttacaaaa agatttgttt
     3181 tagttaaatt tttatttgct ttatcaaatc tttcttcttc atgtttttca gtactaaaag
     3241 ctctgataac tggaatacca gtaattattt cacggcttac taagtttaac ttatcaacaa
     3301 gcttttgaac cagtttaaac ttaggcataa ccactgagaa taacactaca attaatgata
     3361 agatagcaac tactgctact gctataatcc aggccattga agtatttgtg tttaaaacct
     3421 ttatcactcc acctatagcc attataggtg catagaatac tattctaagc aacataacca
     3481 tcaacatttg tatctgttga atatcatttg tactacgagt aattaatgaa gctgtagaaa
     3541 attcattcat ttcagtattt gaaaatgaca taacttttct aaatactcca cttcttaaat
     3601 ttctacctac acttgctgct actcttgctg ctaaaaatcc tactgttatt gtagctacca
     3661 tactaatcat tgctatacca agcattttag caccagtaaa taatatataa ttattttgag
     3721 ttttttctgt atcaatacct attttctcat attctgacct tacatatgaa acagagcttt
     3781 gtgttatcat actctctggc atactttcaa agcctttgct catctcttta gtcaatgtat
     3841 ctaactgttc ctttggcatt gctgataaca atttaaatat atcaacatct ccactttgag
     3901 tagcttgtgg tggaagtttg ctcattaact gagctttcat agcttttacc tctttactat
     3961 cgccctcaag ccctgatact attaaaatag ctttaccaaa aataacattt aaatcatcta
     4021 tagtgtcttt gtctttagta tttaactcat aaagagattc atcttttaga cctgggtact
     4081 tatctttata cttaacataa tcctttttat tcagcaaagt ataattatct agtactttat
     4141 ccttactact tttatccata aataaagtaa ttttatttaa ttcactctct ctaatcacag
     4201 atggaacaga gttttctact ccaccttgtt gtatacctac atttactata ttggatgtgt
     4261 attctggtaa agaaaggtca cataccgctt gaattaccaa tagacagact attattacta
     4321 ttgataatgc cgatttcttt aaatatttaa ttaatttaaa catacatttc ttcccttctg
     4381 taatattaat tagtctaata acattaaatt gaaaaacatt attttaaact ttaaaaatat
     4441 ttttcatctt catgcttatg acatcctgct tttaaattat ctcgtacttg cattagaagt
     4501 cttctaagta ctattttttc ttcttcacta aatcctaaaa aaaactctct ttccaattcc
     4561 ttgttaaatt cttcaacttt attacatatt tctaaacctt tgtctgtcaa aaaaatccta
     4621 gatactcttt ggtccttttc atcttgtttt ctttccacaa gtccagcttt ttccatcctc
     4681 tttatcataa ctgtagttgt agatgctttt actttccttt tatctgctaa ttctttttga
     4741 ctttgcccat ctttcatctt taaagaaaaa agtatttggg gttgaccatg atataagcct
     4801 aatttatcaa aataaaaatg agttcttata aaatatattc gcattaattg caagaaaaca
     4861 tcatttaatt tttcttcaga attgctattc atacttgccc tccttatttt ttatattact
     4921 tagtcagcta agtattattt tactatactg catgcatttt ttcaatatat ttataaaaaa
     4981 ttcatataaa tttcatttat taaatatttt tatatcatat agttataatt ttaataaata
     5041 atagtaagat ttttaattag tcagctactt atatttttta ttgcaaagct taaaataaac
     5101 cttttcatat attttcaaat aaaaaagttg tcccaaaata aattttgaaa caacatcata
     5161 ttcaacagta tcttgattat ataacattat gatactctac ttatcttcat atccatttac
     5221 aacaacgtct aacttcccag tttcaggtga tataattaga ccatgtacaa taataccatc
     5281 tggaattaaa ggatgccctt ttattaaatc aatactatct tttacagatt cttctgcaga
     5341 atcgaatcca tgtaaccatt gttttgtatc aattccagca ctacgaagtg taagtattac
     5401 atcattagat attcctctat ctaaaatttt accaagtaac ttatccgtat caacattaca
     5461 cataccacat ccatgatgcc ctacaaccat aacctcatct acatcaaatt cgtaaattgc
     5521 aactaaaaca cttctcataa tacttccaaa aggatgcatt atagtagctc ctgcattttt
     5581 tattagtttc acatctccat ttttcaaatt catagcctta ggcaaaagct cagttaatct
     5641 tgtatccata caagataaga ctactatttt tttatttgga tgttttgatg tcacatattg
     5701 ttcatattct ttgttctcta caaatgattt attatattct aatatctctt caagttttct
     5761 catatgtaat atcaccctct ttaa
//
LOCUS       DAEOSP010000206         5723 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.210, whole genome shotgun sequence.
ACCESSION   DAEOSP010000206 DAEOSP010000000
VERSION     DAEOSP010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.210"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            57..722
                     /locus_tag="KRH54_003119"
     CDS             57..722
                     /locus_tag="KRH54_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580753.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFSAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            complement(1228..3930)
                     /locus_tag="KRH54_003120"
     CDS             complement(1228..3930)
                     /locus_tag="KRH54_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG8580754.1"
                     /translation="MKNVSKKFFVCTIIVLITFLCFSKFAIYVFDKQEKKETKVVENM
                     LNVYKNNIILNLTEKFNYSKDLTSVLVSNNYSIDKLKPIADEILKNNSCIYISVVEKY
                     NVAAFFSNSVKKDIINTPIQELNYSYSLANSIKGLVVEGPSKDQFSNGDVFLITNPIY
                     KNGEYWGEITVALDAKKVVDSFNLNKLKESGFEYELWRVHLDNGSKSTIEISTKDFNF
                     SKSHEIKFKLPTVWTLSIVYKNGWNNKNIDFIVFVILLIISILIGVMTFIYIEKKSIS
                     KELNQQSLKNLETGILNREGFLIKLQEHLNRKNNEIVLLYICITNYSKFAPVMSFEKR
                     QDYLTHIVKNLDNYIKSSHFIARISEEGFVLALEENCDKNELLNIEKGISLELLRKIK
                     VENKQVFLTIGYGSSRCVDDRKDAESLLLSAIEDYERRFPAMSPIHDFTKKCEDMLDE
                     KKQIVFGEYPDLEINKLSKVIYKYYKKVQRIMYKDYNIDIGNRRGYMRDVEVLIDYNS
                     ERMLYLFLIDICKFGKFNELFSIEIGDVILKEVSNKLKTIFSNYLYRINGDVFLGISI
                     DKESYLDTLHKVNEVFNSSLSVEDVLFDIKCNIGICQYPYHGENPYDLLEKVQIALEY
                     AKKDVKSNVKVYDKELTEEIYHENNIIKDLKKGLEEQTLEVWYQPIFNVSKNCYIKAE
                     ALIRLRNKEGGYYSAYEVITYAEKHGLIKDVGLYVLKKACDVTTKIKDKNIDFEQMQL
                     NLSVQQIVHKQCANDILSCIENCGLDKKYICVEVTETVLMETFEIVNVTLDKLKKEGI
                     SIALDEFGSGFSSINYLANLPIENLKIDKELVEQITYSKKQFELLKTIVQIAKINGMD
                     IVAEGVETKETLDLVVTSGVNLIQGHYFSEPLSEDKFIELMIEE"
     gene            complement(3956..5506)
                     /locus_tag="KRH54_003121"
     CDS             complement(3956..5506)
                     /locus_tag="KRH54_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBG8580755.1"
                     /translation="MRYKKRNIDKSSELSFQSLAKYFITFYIVIILIILSMFCINTIW
                     VMKKDNQDLLSTTSNRIEDRVGETIKLLNSLSKDNYIIDNSVNGIDKSKELSMYSDEF
                     GYMMICFVDKNIIVWDEEGGNISLAGRDYMQKLFATKKYQVTDSFAAGADGKTLNYTI
                     ASPIIKNGNVDGCVFAAIYFDEIKDILKQGKGQQQQNFILFGSENQIMNLTSDETAYG
                     EKYMKLSQKTKYLGTNAKKVEKDMFNNKSGDYWAFENGKLYFMSYKPIKNTNWSLFCK
                     VNFLDIFALNLIPMGAILSVITLVFIILICMEKSFMKKRMEVIDMLLGSVQELEKRIY
                     QTERPSEVDFKEIIQLTSKGLTDGLTGVVTRTVFFDQIYNRVEMEENDKLSVISFVDL
                     DDLKCINDLYGHEIGDMVLKNVGYVLREYEIKYNGLIGRYGGDEFVMLLSGFTSREEV
                     EDLLDEIAIRLHTEVLTDDGIIQTHCSVGAVVCSVKDNKDIDNLLKQADEALYYVKRN
                     GKGNYHLV"
BASE COUNT         2057 a          930 c          553 g         2183 t
ORIGIN      
        1 taatataagt tttataaagt atattaaata tattataaga tggaggtcga gtaattttga
       61 aaggaaacat agtacaatat aattttgcag atatagagga agaagtatat tcgttggatt
      121 atgctatagc atggaataca gacgaggaaa acgtaaacat aataccattt acaaataaat
      181 tctgtaaaga atctatagaa tctttttgcc taggtaaaat taataacttt gttgaaatat
      241 taaatgaagg ttttgttgaa aatcatcatt atgtacattt agataaaatg attagtgttc
      301 ctaagaaaaa ggttaattta gtatatcaac aagacacaca tggttatctt ctaagagatg
      361 ataatgacaa ccttattcct gcaaaaataa cttcagagca atctaaatct atatcaagta
      421 agatggagtt attctctgct ggtgaagaaa aatgtttaat caatatatta ctaaaggctg
      481 acccatcata tattttagat gtagatagta taaaagacaa aaacattcta aatctaggtt
      541 atgagtctat tgacagatat aaagaataca attttgatga tgataagatt ttaatatttt
      601 ttataaataa aaaacgttat tcagtgatta tgaaaaaaac aaacaactct gataacgatt
      661 tagtttccag aaacaatgct attaaagaat tatttactaa taaagcagga aatcttaact
      721 aataatataa ttttaaatta tgttattgat taaattaata atagtataca aagaggctgt
      781 ctcaaaatag agataagctt ctttttcatt gttgaaataa taaaatatat ttaattctat
      841 ataaaagaga gtgcctcata aactaaaaag ttcattttga gacaccctcc tatttataat
      901 aaacaaataa atccttaaac ctatcgagtg attataattt ttagattggg ttatcttgca
      961 tttttaaatt taaggtaact ttctactcta aactttaatc tctaatcaat atcatcaact
     1021 gtaacaactt acaattaaat ctttaataat caaactttag actatctata ttaaaactca
     1081 aattagattt aggatttatt tggatatcac atttatttta ttaactacta atcaattatt
     1141 tttacttata aacttattct tccctaattc tattattaaa tacttttaat tatttttcaa
     1201 taccttaaat ctttatttta ttaaccttta ttcttcaatc ataagttcaa taaatttatc
     1261 ttcacttaaa ggttctgaaa aataatgacc ttgaatcaaa ttcacacctg aagttacaac
     1321 taaatccaga gtttcttttg tttcaacacc ttctgctaca atatccatgc cattaatctt
     1381 agcaatttga actatagttt ttaataattc aaattgtttt ttagaatagg taatttgttc
     1441 tactaattcc ttatctattt ttaaattttc aataggtaaa tttgccagat aatttatact
     1501 actaaatcca gaaccaaact catctaaagc tatgctaatg ccttcttttt ttagtttatc
     1561 aagagtaaca tttacaattt caaaagtttc cataagtact gtttctgtaa cttcaacaca
     1621 aatatacttt ttatctagac cacaattctc aatacatgat aatatgtcat ttgcacactg
     1681 cttatgtaca atctgctgaa cagataaatt taattgcatt tgttcaaaat ctatattttt
     1741 atcttttatt tttgtagtta catcacaagc cttttttagt acatatagac ctacatcctt
     1801 aataagtcca tgcttttctg cataagtaat aacttcatag gcagaataat aaccgccctc
     1861 tttatttctc aatctaatta gtgcctcagc ttttatataa cagtttttac taacattaaa
     1921 aattggctga taccaaactt ctagtgtttg ttcttccaga ccttttttta aatccttaat
     1981 aatattattt tcatggtaaa tctcttctgt caattcctta tcataaactt taacattact
     2041 tttgacatcc ttttttgcat actccaatgc aatttgtacc ttttccagaa ggtcatatgg
     2101 attttcacca tgatatggat attgacaaat accaatatta cattttatat caaaaagtac
     2161 atcttctaca cttaaagaac tattaaatac ttcattaacc ttatgtaaag tatctaagta
     2221 agattcttta tctattgaaa tgcctaaaaa tacatctcca ttaatacgat atagataatt
     2281 agaaaaaatt gtttttaatt tgttactaac ttcttttaaa ataacatctc ctatttcaat
     2341 agaaaacagt tcattaaatt ttccaaactt gcatatgtct attaaaaata agtataacat
     2401 tctctcagaa ttataatcaa ttaatacttc gacatcacgc atataccctc gtctatttcc
     2461 aatatcaata ttataatctt tatacataat acgctgtact tttttataat atttgtatat
     2521 tactttagac aacttgttta tttctaaatc tggatattca ccaaatacaa tctgtttctt
     2581 ttcatcaagc atatcttcac actttttagt aaaatcatga ataggagaca ttgctggaaa
     2641 tcttctctca taatcttcaa tagctgaaag taacagagat tctgcatctt ttctatcatc
     2701 aacacaccta gatgagccat aaccaatcgt taaaaatact tgtttatttt ctactttaat
     2761 ttttcgtaaa agctctaagg aaatcccttt ttcaatgtta agcaattcat ttttatcaca
     2821 attttcttct aaagctagaa caaaaccttc ctcacttata cgagcaataa aatgagaact
     2881 ttttatgtaa ttatctagat tttttactat atgtgtaaga taatcttgtc ttttttcaaa
     2941 gctcataact ggagcaaatt tactataatt agtaatacat atatacagca acactatttc
     3001 attatttttt ctattcagat gctcttgtaa cttaattaaa aaaccttctc tattcaaaat
     3061 accagtttct aaatttttaa gactctgttg atttaattcc ttactaatcg acttcttttc
     3121 aatgtaaata aatgtcatca cacctattaa tatggaaata attaacaata taacaaatac
     3181 aataaaatct atattcttat tattccatcc atttttgtac acaatgctaa gagtccaaac
     3241 agtcggaagt ttaaacttaa tttcatgtga tttggaaaaa ttaaaatctt tagtagaaat
     3301 ttctattgta gattttgaac cattatctag atgtacacgc caaagttcat actcaaatcc
     3361 agattctttt aatttattta agttaaaaga atctacaact tttttggcat ctaaagctac
     3421 tgtaatttct ccccagtact ctccattttt ataaatagga ttagtaatta aaaacacatc
     3481 tccatttgaa aactggtctt tagaaggacc ttctacgact aatcctttga tagaatttgc
     3541 taatgaataa gaataattca gttcctgtat tggagtattg ataatatctt ttttgacaga
     3601 attagaaaaa aatgcagcta cattatattt ttctacaact gaaatatata tgcaagaatt
     3661 gtttttaaga atttcatctg caataggttt taatttatct atagaataat tgtttgaaac
     3721 taaaactgat gtcaagtctt tgctataatt aaatttttca gtcagattta aaataatgtt
     3781 attcttgtag acatttaaca tgttttctac tacttttgtt tctttctttt cttgtttgtc
     3841 aaaaacataa atagcaaatt tactaaaaca taaaaatgtt attaatacaa taatagtaca
     3901 tacaaaaaat tttttagata catttttcat atgatataaa ctccttccac atacattaaa
     3961 ctaaatgata attacctttt ccattacgtt taacatagta taaagcttca tcagcctgtt
     4021 ttaataaatt atctatatct ttattgtctt ttacactaca tactacagct ccaacactac
     4081 aatgagtttg aatgatacca tcatctgtta gtacttcagt atgtaagcga atagcaatct
     4141 catccaataa atcctccacc tcttctctcg atgtaaaacc agataacaac ataacaaatt
     4201 catctccacc atagcgccca attaaaccat tatattttat ctcatattca cgaagtacat
     4261 atccaacatt tttcaaaacc atatccccaa tttcatgtcc atataagtcg ttaatacatt
     4321 tcaaatcatc taaatcaaca aatgaaatta cagataattt atcattttcc tccatttcaa
     4381 cacgattata aatttggtca aaaaatacag tacgtgttac aacaccagtt aatccatcag
     4441 tcagaccttt acttgtgagc tgaataattt ctttaaagtc tacttcacta ggtctttctg
     4501 tttgatatat acgtttttct aattcttgaa cagaacctaa aagcatatca ataacttcca
     4561 ttcgtttttt catgaagctt ttctccatac aaatcaaaat aataaatact aaagttataa
     4621 cagatagaat agctcccata ggaattaaat ttaatgcaaa aatatctaag aaattaactt
     4681 tacaaaataa actccaattt gtgttcttaa ttggtttata ggacataaag tataattttc
     4741 cattttcaaa agcccaataa tcacctgatt tattattaaa catatccttt tctacttttt
     4801 ttgcattggt tccaagatat tttgttttct gcgataactt catatacttc tctccatatg
     4861 cagtttcatc acttgttaaa ttcattattt gattttcact tccaaataat atgaaattct
     4921 gttgctgttg accttttcct tgtttgagta tatcctttat ctcatcaaag taaattgctg
     4981 caaatacaca accatctaca tttccattct ttattatagg tgatgcaata gtgtagttta
     5041 aagtttttcc atcagcccct gctgcaaaac tatctgtaac ttgatatttt tttgttgcaa
     5101 aaagtttctg catataatca cgaccagcta gactaatatt accaccttct tcatcccata
     5161 caattatatt cttatcaaca aaacatatca tcatataccc aaattcatca ctatacattg
     5221 aaagttcttt acttttatct atgccattca cactattatc tattatgtaa ttatctttac
     5281 tcaaagagtt caatagctta attgtttctc ctaccctatc ttcaatacga tttgacgttg
     5341 tagataataa atcttgatta tcttttttca taacccatat tgtatttata caaaacatag
     5401 aaagaataat taatataatt actatataga aagttataaa atattttgct agagattgaa
     5461 atgaaagttc acttgactta tctatatttc tttttttgta cctcataatt cctaccccca
     5521 ctatagaaaa attgatgaaa atgttcctat tagaacctta ataatacatt acaaaacaca
     5581 taatttcaag taaaacgtta aattttcatt actaaatata aactttttat tacaatatta
     5641 ttttttatat cctattatag ccaatactat cacatattta gtaattatat ctattcattt
     5701 tacaaatttt gatacgtaat tta
//
LOCUS       DAEOSP010000207         5667 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.211, whole genome shotgun sequence.
ACCESSION   DAEOSP010000207 DAEOSP010000000
VERSION     DAEOSP010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.211"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            123..1448
                     /gene="cdeA"
                     /locus_tag="KRH54_003122"
     CDS             123..1448
                     /gene="cdeA"
                     /locus_tag="KRH54_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBG8580756.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(1543..2337)
                     /locus_tag="KRH54_003123"
     CDS             complement(1543..2337)
                     /locus_tag="KRH54_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8580757.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMIIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2338..3213)
                     /locus_tag="KRH54_003124"
     CDS             complement(2338..3213)
                     /locus_tag="KRH54_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580758.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3217..3585)
                     /locus_tag="KRH54_003125"
     CDS             complement(3217..3585)
                     /locus_tag="KRH54_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8580759.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3768..4427)
                     /gene="deoC"
                     /locus_tag="KRH54_003126"
     CDS             complement(3768..4427)
                     /gene="deoC"
                     /locus_tag="KRH54_003126"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBG8580760.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(4463..5650)
                     /gene="murJ"
                     /locus_tag="KRH54_003127"
     CDS             complement(4463..5650)
                     /gene="murJ"
                     /locus_tag="KRH54_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBG8580761.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTVFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKRKLH
                     LKK"
BASE COUNT         1889 a          882 c          632 g         2264 t
ORIGIN      
        1 tttatcttaa tatgattttt tgttgatttt aagctgttta tcgtataaat aaaatttttc
       61 ttgactatat agttactata tagcttatta tatatagaga gtaataaaag gaggtaaaat
      121 ttatggaaaa tttatttaca agaaaattca ctacttttga atttctaaaa tttgtttctc
      181 ctgcaattat atccatgata tttatatctt tgtatacaat aatagatggc atctttgtat
      241 cgacattagt tggttctgat gctcttgcta gtataaatat tgtactacct ataattaacc
      301 ttgtttgtgg atttggaata atgatggcaa ctggtggagg tgctatcgtt tctatacgta
      361 tgggtgaaaa tagacaggat gaagccaact ctacattttc ttttatagtt ttgttttcat
      421 tgattgttgg gattttattc acagtaatct catatttctt tatcaaagaa atatctattt
      481 tgcttggtgc aacagataag ttattaccat attgtataac ttatggtaaa gttatgattt
      541 tatgtacacc attttatatt ttaaaattta tatttgagta ctttgcaaga actgatggaa
      601 attctaaatt tagtttattt ctatcagtca ttggtggtgt aacaaatata atattggatt
      661 atgtatttat taaatatttt ggaatgggtc ttttaggagc tgcagttgca actgctatag
      721 gtattatttt aacttgtgtt ttaggtatta tttacttctt atctaataaa tctacactaa
      781 aattaagaaa accaaaaacc gattttagac ttataagaga tactatgata aacggttctt
      841 ctgaaatggt tacagaatta tctacaggaa ttacaacatt cttatttaat gtagtagctt
      901 taaaattagc aggagaaaat ggacttgctg ctcttactat agtattgtat gctcattttt
      961 taatgacatc agtctatcta ggattcgctg ctggagtgtc tccattaata agctataatt
     1021 ttggtgctga aaacagtgat aaattaaaag aaacatttaa acattctcta aaatttatat
     1081 ttgtttcttc tcttttagtg tttattattg ctttagtatt tgcaccattt attgttaggg
     1141 tctttgtaag tccagataac acagtattta aactagcctt acaaggatta aaaatatttg
     1201 catttgcttt tttgtttgtt ggtataaata tatttgcatc aggatttttt acagcatttc
     1261 acaatggaaa aatttcagct attatatctt ttagtcgtgc ctttgttttt ataatcatag
     1321 gaatcataat tcttcctcct atgttaaaca tgactggatt atggcttaca gttccatttg
     1381 ctgaggttat aaccatattt atatctattc tatttataaa aaaatataaa tgtagatata
     1441 agtattaaaa attaatactt aaaatcaaca tttattaaaa ctaataaacc tgctttaaaa
     1501 tacaaattta caaatttgat ttttaagcag gttttatttt agttataata agtcatttgc
     1561 atactcttta attggtgctt ttctaagtaa taaaattata aatattatgc aaataactat
     1621 aattcctacc tttgttccaa ataaaataaa aggattctga aatgaataaa taattttttc
     1681 taaatacaat agcattttat gcatattttt agttgcaaaa ttaactatat aaattatcat
     1741 taatatcggt aatcctatca atatagtaat actagtaact tttctaactc tatatgtaaa
     1801 agctccaagt aacactccta taaaacctat acatatttca tttatacata tttgtacaaa
     1861 ataatttaaa taagttagtg gcatacttga tgttgaagga tctatcagtg ctgatataga
     1921 tgtccaaatc atatctctct ttagtaaaac tgatagttct atactgtaac ctgttataat
     1981 ttctataaat aacttcgata aaaaaatcaa aactatacct aaaatagaaa tcacaaagct
     2041 taaaataatt aaatctatga caatagcttt tatacaactt tttctatctg ctcttatact
     2101 taaagctcct gaaaagtctt tatttgctat atttactcca tacacaaata gcagtatagt
     2161 tatatatgtt aaaataggat tcacaatcgt ggataaatta gttttagatg cttctccaaa
     2221 aatcttgaga ataaacactg gcaaaatatt cataacaatt cccacaatca taaatataat
     2281 tatatacaat ttagtatttt tattaaaaaa ttttatatat ggctttaatt cattcaatta
     2341 caattcctcc ttcttagtca tgttaacaaa catatcttgt agccctatgt atcctacatc
     2401 aatatttgaa tttttaagta ttttttcatc attttcacta aaatcaccat agtaaaaata
     2461 agcgactgca tttccaaatg cctttttagg ttttatattt tttatacatt ctaatttttc
     2521 taactcttct ttatttccag taatataatg agctttttgt tttatgacat caatttcttc
     2581 atctataata atttttccct catttaatat tattacatgc tccagtaaat catctatttc
     2641 atcaatcaaa tgagttgata ttattattgt ccttggattt tttatataac tatctaaaat
     2701 tatgttataa aactcttgtc tatttactgc atccagacca attgttggct catcaaatat
     2761 ggtgatagct gaattagaac aaatacctat tatatttgaa agtatagttt tcatacctct
     2821 tgacagttgt ttatactttt ttttagtatt taaatcaaaa tatttgcata attcttcttc
     2881 taagccttta tcataacttt tataaaaact agaagaaaat tcaaatatct gacctacttt
     2941 aaaatctgga ctataaaatt ctttttccct gacgatacaa acttcttcca aaacttttat
     3001 atcttctttt gggttctttc ctaatatttc tatctcacct tcattttgaa ttaactgatt
     3061 taccattata tttaataatg ttgtttttcc agctccattc ttacctaata aaccatatat
     3121 cttatttttt tcaaagttta gagacatatt attaaaaata ttttgcttct taaaactgtg
     3181 agataaattt tctatcttaa tagaactact catatattaa tcctccttga aattaattat
     3241 aatatcaacc aattcttctc tatttatttc taatttgtta gcttcttgaa gtaggttttt
     3301 tacaaaatta tgtttaaaat tttcttttct agcttctttt ataatagttt ttgcaccatc
     3361 tgatacaaac atccctatcc ccctcttctt atataaaata tttttatcaa ccaatatatt
     3421 tatacctttt aatactgtag ctggattaat tttataaagc tttgaaagct ctgttgttga
     3481 tggaacctgt tcttcttctt ttattccatt tgatagtatc tcatcttcaa ttgctcgtgc
     3541 tatctgaata aatataggtt cttcattatt taatactaat ttcataggtt cctcctttct
     3601 tcatcagtta gttactcaac taattaacta tataacttat aatatcttga aaaatatttt
     3661 ctgtcaatac taaataaaaa acagagggaa aattccctct gttttttgtt ctttatattt
     3721 ataattaaat tttgattttt gtaatcctat cccataagtt aaaaatttta ataatctgtt
     3781 gtatcttcat tttttaatat tttacatatt gagctagttc ctaacctttc tgctcctgcc
     3841 tctataaatt tttctgcatc agaaattgat ttaactcccc ctgcagcttt tatttttaca
     3901 ttttttccaa catgctcttt cataagcttt atatcttcta aagtagctcc acctgttccc
     3961 attccagtag atgtttttat aaaatctgaa cctgacattg ttactatttc acacattttt
     4021 attttttcat cttcatctaa ataacatgtc tctataatga cttttaatac tttatctcct
     4081 atagcttttt ttatttcttt tatttctttt cctatattat cataatcttt attttttata
     4141 tctgaaatgt ttacaaccat atcaacttca tctgctccat tttcaatagc atcttttgct
     4201 tcaaatactt ttgtagcagt agtttgatat cccaaaggga accctatcac tgtacatatc
     4261 tttattttac cttttagata ttctgatgct cttttcacat atgatggtgg tatgcaaact
     4321 gatgctacac tcatatctac agcttcatca cataaaattt taatatcttc ccatgtagtt
     4381 gtagctttta aaatagtatg gtctactgtt tttaatatat gtttcataaa atcctcctaa
     4441 atttataatt aatataatta gattactttt ttaaatgcaa ttttctcttt gcaatattca
     4501 gtatcatatc aacctcttca actttaaaaa tagtcattag tagtgtataa attccacctc
     4561 caactataac tgaaatagct agtgatacaa actcattaaa tgttcctact cccaaaattc
     4621 caaatataaa tttatacgta aagtatgata aaacacccat aattaaagat gcaactaccg
     4681 atttcaaagt tgcttttatt attttatctt gtccaaaata tccaactttt ctctttaggt
     4741 tcaagaagag taacaatata catgcaattg atgatgaact agttgcaaga gcaagaccac
     4801 catgagccat tggttttatt aaaactaagt ccaatactat atttacccct acagatataa
     4861 tcccatttac cataggtgtt tttgtgtctt gtaatgaata aaaaaccttt cccaaaatat
     4921 ctcttaagcc aaatgcagtc ataccaactg cataaaaaat taaggcagtt gctgtcattt
     4981 gggttgccct cgcatcaaaa gcacctctct caaaaatgat tctaacaaca ggagttgcaa
     5041 aaaaaataga agcaactgaa ataggaatca tagaaattat tatcatattt atactggatt
     5101 taactgaact agtaaacttc ttttgattat tttctgcact taacttagat aacataggat
     5161 acataacaga tactattgaa gctgtaaatg tccctgtaac aaaaccattt aatctatctg
     5221 cataagtaag tgctggaatg cttcctttaa ctaatgttga ggctaaagtt gtatctacca
     5281 atgagttcac ttgattaaca gcaacaccta taaatactgg tgaaagtaat gctagtaatt
     5341 taataaggct atcatcttta aagtttaagt agtataagta tttgtagttc gtttttttaa
     5401 caaaaaacat gtaaaagagc agttgaacta ccatagcaac cacagcacca attggaagta
     5461 tgtatggtcc aaatacggta cttaacatta ttgaaataat tattactata ttatagggta
     5521 tacttccaaa tccaactaca ataaagttct ctttaatttg taaaaatgct gacataacac
     5581 tcgtaatacc tatgaatatt attcctgttc tgaacctccc atgactaaag tcacagggtt
     5641 cctgcttcat agaattttgc atactaa
//
LOCUS       DAEOSP010000208         5649 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.212, whole genome shotgun sequence.
ACCESSION   DAEOSP010000208 DAEOSP010000000
VERSION     DAEOSP010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.212"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..800
                     /locus_tag="KRH54_003128"
     CDS             <1..800
                     /locus_tag="KRH54_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG8580762.1"
                     /translation="VSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGK
                     IVQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEI
                     ISYINNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIKNN
                     DICSIFANSLDNAIEACMDINNEIEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRI
                     QTSKDDDNIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            812..952
                     /locus_tag="KRH54_003129"
     CDS             812..952
                     /locus_tag="KRH54_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBG8580763.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            1011..1592
                     /locus_tag="KRH54_003130"
     CDS             1011..1592
                     /locus_tag="KRH54_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBG8580764.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNILVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFVVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNSDQ"
     gene            1849..2223
                     /locus_tag="KRH54_003131"
     CDS             1849..2223
                     /locus_tag="KRH54_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580765.1"
                     /translation="MNLKKKIKKLGVTFAIGTIIVSSLGTSVFADEVSTYALISKAFS
                     GKNNIISSSVYTSGSRTFKTTDSQGGFSCRLTERFSGSSPDRIVKRHEANASSKVSLS
                     GGTYYIRYEGSTNNAKTYATLN"
     gene            2306..4015
                     /locus_tag="KRH54_003132"
     CDS             2306..4015
                     /locus_tag="KRH54_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580766.1"
                     /translation="MSSIKVISDFITKIIRKNILFAITIIIINYHLIQRWTHISNRGI
                     SFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTVKILCRNNFDYFLGISVAVTRNNF
                     IFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILIFEWFLPLTILSIVISFISVIINNLI
                     LKITLSSVFLYLTSTNMLDRVIDIKEPFFKALFKSLNIFEDQSYAYYSDFVGKVNNSA
                     YVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLIVIVVYSILFIGLNYISIDMSTYR
                     DEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNIKDRIVLLNTSNEELTNINMILDE
                     IFDIKYIKINSENIKFNKKKDLIEVNLKESLEPNDEVTLDIEYEGYINVLTSWNQNKY
                     IATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSKNNVYSNLPLLNKTSNFLKKDYIF
                     EGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMDIEEKIKFLIETRNEILGDSKASV
                     DAKKYVDEVDNVMESIKRKKINMILVADLPSNEFLYEDSKGEKISITIYHWLSGNILI
                     IDK"
     gene            4030..4902
                     /locus_tag="KRH54_003133"
     CDS             4030..4902
                     /locus_tag="KRH54_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8580767.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLTDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYVPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINKKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            4903..>5649
                     /locus_tag="KRH54_003134"
     CDS             4903..>5649
                     /locus_tag="KRH54_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580768.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYSGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVF"
BASE COUNT         2340 a          505 c          833 g         1971 t
ORIGIN      
        1 tagttagctt attacttata tttgaatata atttaagagt caatacaatt actacagaaa
       61 acttatttat atttatattt gcaattttat tgattttatc atcaagtata atacttttaa
      121 tagtaatagg gaaaattgta caagacgata aattaaatct agagtatgaa cttataaatg
      181 aaagaataaa aacaaactac aggaattatg aaaatataaa cgaagttcat gatagattaa
      241 aatatgttta tcatgacttg aaaaaccata tgatttgtat aaaaaattat gacactaaag
      301 aagaaattat atcatatata aataacttag agtttcagat aagtgaattt gaacatttta
      361 aaaatactgg aaataaaaca ttagatttaa ttttagcaga aaaaataagt atttgtaaaa
      421 agtatgatat tcaaattgaa gataacataa atatatctaa attaaacttt attaaaaata
      481 atgatatatg ttcaatattt gcaaattcac tagataatgc aattgaagca tgtatggata
      541 taaataatga gatagaaaaa agaatagaag ttaaagctac atatataaat aaatttgcaa
      601 taataaaatt tataaatact aaggtaaatg atattaaact tattgataaa cgtattcaaa
      661 ctagtaagga tgatgataat atacatggaa taggacttgc tagtattaaa tatatagtaa
      721 ataaatatgg tggtgaaaca atagttaatt actcagataa tgagtttata ttaaaaataa
      781 tgataccaat aaaaagttag gagagtacaa aatgaaaaaa atagcgttaa acttacttaa
      841 aaacatctca gcattaagtt ttgggatagc tgtattatca gcaaacagtg caagttcttg
      901 ggtagcacat caggcaaaag agccacaagc tttacaaaaa ctaaaaaaat aacattaata
      961 aaaggacctc ttcttattaa aggagaggtt ttacatgttg aggaggaaat atgtttaaaa
     1021 gattatcata taaatttgcc aatatattag ttaataatga gattgtagaa agtgaagatt
     1081 ttgaaatata tagatatgga tttgaaactt tgatttattt catagttaat attttggtag
     1141 cattatttat aggaattatt tttgatagat ttatacatac agttatattt ttaagttgtt
     1201 attgcacttt aagacagttc acaggaggtt atcatgctag gaattataaa gagtgtacat
     1261 taacatttgt agttatttat ttgataacta ttttttcagc taataatata gatataaata
     1321 aatataagta tttattagta ttattaatga ttattagtat actaacaatt tacaagttag
     1381 ctcctttaga acatagaaac aaacctctta gtgaaagtga gaaaaagcat tatagaaaga
     1441 ctgtgcaaaa aatactattc gtaataatat gtttaattat tttatgtaaa atacttaata
     1501 tttttcagca atatgtgatt tatgcaataa tttctatata ttggattgct atattaatat
     1561 atattggaat gaaggttaat agtgatcagt aagattataa aacattttct aaattaatta
     1621 acttcactaa ttaatattaa ctttaaaaaa ataatgtcaa ggtaagtatt tgaacttatt
     1681 aaatatagtt gaatctattg tgggttgtat caaaggacaa atagattcac ttaataacca
     1741 gtttgtaaca gagtattgaa ttgtaaaagt taatacgata gtatgaaaat gtaatttgaa
     1801 attacatttt tataaaaatt atattaacta aggggaggga tacatacaat gaatttgaag
     1861 aaaaaaatta aaaagttagg agttacattt gcaataggaa ctataattgt atcaagttta
     1921 gggacatctg tatttgcaga tgaggttagc acatatgctt taatatctaa agctttttca
     1981 ggtaaaaaca atattatatc tagttcagta tacacttctg gaagtagaac ctttaaaacg
     2041 acagattctc aagggggatt ttcttgtagg ctaacagaaa gattctcagg atctagccca
     2101 gatagaattg taaaaagaca tgaagcaaat gcttcttcaa aagttagttt atcaggagga
     2161 acatactata taaggtatga aggttcaacg aataatgcta aaacatatgc aactttaaat
     2221 tagtattaaa agcatcctat aattattttt ataggatgtt tttgtaattg ttataatcaa
     2281 aagactggct aggaggggca taaatatgag tagtattaag gttatatcag actttataac
     2341 caaaataatt agaaaaaata ttttatttgc aataacaatt attattataa attaccatct
     2401 catacaacga tggacacata tatccaatag aggaatttca tttaatgatt acttatcctt
     2461 gagtgtatat acttgtatga caattagtat tttaaccatg ttaaatgtaa ttttttatat
     2521 gagaaaaagt tatgatactg taaaaatatt atgtagaaat aattttgatt attttttagg
     2581 tatttctgtt gctgtaacta gaaacaactt tatattttgt tgcataccaa ttatatatgc
     2641 tatagcatac tcaagtttaa taagaacatt gtcaattgta acgattaaat caactatatt
     2701 tatattgata tttgaatggt ttttaccact tactatttta tctatagtga tatcatttat
     2761 ttcagttatt ataaataatt taatactaaa gattacctta tcatcagtat ttttatattt
     2821 aacatcaact aatatgttgg atagagttat tgatattaaa gaaccatttt ttaaagcatt
     2881 atttaaatca cttaacatat ttgaagatca atcatatgct tactattctg attttgtagg
     2941 gaaagttaat aattcagcat atgttataga taagattata cctacactat ttgcaattac
     3001 tttaataata gtagcatatc tgatattaaa tgatattaga aagaaacaga ttaaatcatt
     3061 aatagttata gtagtgtaca gtattttatt tattggatta aattatatat caatagatat
     3121 gtcaacatat agagatgagg agcgtagtta tggagagtat ttaaagaaat ctaatattag
     3181 tgatattaaa ataaaatcgc atgatatgaa gattgacttt aaaagcaagt caaatataaa
     3241 agatagaatt gttttattaa acacatcaaa tgaagagtta acaaatatta atatgatttt
     3301 agatgaaata tttgatataa aatatataaa aataaattcg gaaaatataa aatttaacaa
     3361 aaaaaaggat ttaattgaag ttaatttaaa ggaaagttta gagcctaatg acgaggtgac
     3421 attagatata gagtatgaag gctatattaa tgtgttgaca tcttggaatc aaaataaata
     3481 tatagctact aaagatgata ttatgcttcc accaggttct ttagcgtggt acccaaaagt
     3541 aaatcaatca agttgtatgg cttataaaat aaatttaagt tctaaaaata atgtctattc
     3601 aaatttacct ttactgaata agactagtaa ttttttaaaa aaagattata tatttgaagg
     3661 aaagtcaaat gatattgcaa tatatgcagg tgagtttaaa gaagaggtaa aagatgatat
     3721 aaaaataatc tatccatcag atagtaatgt aaatatggat atagaggaaa aaattaaatt
     3781 tttaattgaa acaagaaatg aaatattagg tgatagcaaa gcatctgtag atgctaaaaa
     3841 atatgtagat gaagtagata atgttatgga atcaataaaa agaaaaaaaa ttaatatgat
     3901 attagttgct gacttaccta gcaatgaatt tttatatgaa gactcaaagg gagagaaaat
     3961 tagcataact atttatcatt ggctttcagg aaatatcctt ataatagata aataataaag
     4021 gaggttaata tgataaacaa gttagaaata agaaatctaa attttaatta caaaaataaa
     4081 aaagctctgg ataatataaa tttaacccta acagatggag tagttgcatt attaggtcct
     4141 aatggtgctg gaaaatcaac attaatgagg ttattagtga ctctttatga aacttcaatt
     4201 ggagagatag aattaaataa tataaagtat agcaaaaaca atgagaaaat caaggcaaat
     4261 gtaggatatg tacctcaaga ttttgatatg tataataata taaatgggca agaatactta
     4321 gaatttgtag ctaaaatgag aggtgtatct aagaatgatt taaagaaaca tatacaaaaa
     4381 gtagtatcaa aagttaatct ggacaagttt ataaataaaa aaataggtac atactcaggt
     4441 ggtgtaaaga gacgactagg tatagcacag gctttaatag gtgattctaa gttaatagtt
     4501 atggacgaac ctacagttgg tcttgatcca gagcaaagga atgaatttag aagattgtta
     4561 ccagaaataa gtaaaaattc tatagtatta atttcaactc acatagtaga agacatacaa
     4621 tttaactgtg acaaattaat tatattaaat caaggcaaaa tattatatga tggtacaatt
     4681 aataagttta ttgatatggt tgaggtgtat tcggtagtag tttctaatga tgaatttgaa
     4741 tatttagaac gtaatattga tattattgat tataggagag ttaaagatgg tgtgcaagta
     4801 aagtatatag gtaaaaatac agagaaactt aaagatagct taaggataga tacaacttta
     4861 caagatgctt atataaactt tttacataag gagttaaaat aaatgtgtat attggagtta
     4921 ataaaatttg agcttaaaaa aatagagctt aaacatgtgt taatatttgt aattatttct
     4981 atattatgca tatttattac aggcatatta gattataatg agttaatatt ttataatgat
     5041 gtacctataa tggatacata ttctatagca ggatggttag gaattataaa gatatcagat
     5101 gtttttatac caataatgtc agcaatcaca ataatttatg tgttttcaaa agattattcg
     5161 ggtaatataa atgagatact tactttatat aataaaaaaa aatataatca atttatagta
     5221 tttagatggt gtgttatatt agctatatat actgtattat tattaatttc aattacaata
     5281 gtatatacat ttacgtcagt gaaaggtttt aacatagaca aaatagattt tgtttatagt
     5341 gtaaagccaa tagatgtgtt tttaaagacg tttcctacat tattatggta tacaacattt
     5401 ccactaatga tattaacatt aacaaaaaat agatttacaa caatagcaat attaattaca
     5461 tatacatttg ctgatatatt ttttatttta catatgtacc catttgttag tatgattaat
     5521 gtaaatggat tttatattca aaagatgttt tatatgccta acaatataga tgttagattt
     5581 aatgaattaa agaattattt ctttataaat agaggttctc tagtagttat ttctataata
     5641 atagttttt
//
LOCUS       DAEOSP010000209         5614 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.213, whole genome shotgun sequence.
ACCESSION   DAEOSP010000209 DAEOSP010000000
VERSION     DAEOSP010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.213"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            240..1067
                     /locus_tag="KRH54_003135"
     CDS             240..1067
                     /locus_tag="KRH54_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBG8580769.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            2092..2556
                     /locus_tag="KRH54_003136"
     CDS             2092..2556
                     /locus_tag="KRH54_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8580770.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIGMKPNYCGMGIGKEIIGLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
     gene            complement(2823..3467)
                     /locus_tag="KRH54_003137"
     CDS             complement(2823..3467)
                     /locus_tag="KRH54_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8580771.1"
                     /translation="MFGQSFQNYLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3397..4686)
                     /locus_tag="KRH54_003138"
     CDS             complement(3397..4686)
                     /locus_tag="KRH54_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8580772.1"
                     /translation="MNLFIKECKNILKSMIYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWFYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLEALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(4683..5555)
                     /locus_tag="KRH54_003139"
     CDS             complement(4683..5555)
                     /locus_tag="KRH54_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580773.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
BASE COUNT         2033 a          771 c          699 g         2111 t
ORIGIN      
        1 ataattttaa catgcccata aaatataata cccacaaatg ataaataata ctcaaaatat
       61 ctattgaaat aactatacat ccttaaataa cttaacttaa atctaaacat ttcaaaaact
      121 tcttaaaaat atgtaaaaat ttgatgataa agtagcgatt ttttcaaaaa aaatgtaata
      181 tagagttata tcttataata ttttagagat aaattttatc atagtgggga gaaatttgta
      241 tggatttaag caaaaaagta aaaattttag aaaatgcatc agatggcaaa agcgaatttg
      301 agatattaga atttgaaaaa cttgagggag cttctagccc attaatggca atgggattat
      361 attttgctag agaagctggt ttgaaagcta gacaaattaa gataaaatta aataattcat
      421 caataaaaac agaagctggt gcattatact attataaagg aaatatatct tctgaaacta
      481 agattggtgg tgttggaggt ctttttaaaa aaggaatatc aggagcactt actaatgaat
      541 caatgatgaa acctgtatac aaaggtactg gagaagttta tttggaacct tcttttagac
      601 attatttatt tatgagtctt gataatgatt ctataattgt tgataaagga ttattctatt
      661 gttgctctga tacaatagaa gtaaaagcat ttacacaaaa gaacttctca tctgctttac
      721 ttggtggtga aggcttcttc caaatagaat taagtggtac tggtgtaatc gttctggaat
      781 gtgttgtacc tcaaagtgaa attgtagagt atgaattgaa aaatggtgaa gaattaaagg
      841 tagatggtaa ctttgcaata gcaagaacat ctggtgttaa tttttctgtt acaaaatcag
      901 ataaatcttt aattggttct gctttaaatg gtgaaggttt cttaaataca tttactggta
      961 atggtacagt ttggctagct ccaactcaag taatgtatga aaaactaagt tttggagcaa
     1021 tgcctactaa tgataatatg aataataaag tttctaagca aaaatagttg ttttataaat
     1081 atttattaaa tcattattga ttttataaag tcactataaa ttttattaga ttattattat
     1141 aaaatcagag tgtggaaatg tttatgtgta ttctttaaat aaaaatttcc acattctgta
     1201 aggttattat acttgatata taaaattcaa tttaggaaca tagtctaaca tttataaaca
     1261 tattaaggta atgatttctc ttagtaatta ttctcatatc ttattctttg attatattta
     1321 tcttattcct agcccttatg tcatattata tttcttatct tattatttat aattagcatt
     1381 aacttatcta aaatcttgta tttttttaat agtaaagttt acatctatat taaaaagttt
     1441 acatctatat taatttcata aaacaatcaa ggtagtcaag agattcaact ttatttgatt
     1501 ttaaaaattt aagtgaattt gttcttatac acaccttttt aaaattatat ctgtagaaaa
     1561 tatcatttag agctataatt aaattaactt attaaatttt agaaaattta ttaaaaaata
     1621 taagttaaat taatgttttt aaagattctt ttttgtaact actagaatac taatttagta
     1681 gttattgtat ctctaaagtt taagcatgga tagatataca tttgataatt tgcctataat
     1741 ttacctacta cttggattat tggagcaaat acaatatatt aataataagt tcctaaaacc
     1801 aataaattaa ctaatacaca taaagatatt gatgttggtt atacaactat aagaaacgga
     1861 tttaacagaa tttaatcaag ctaagaaatt atatatatat cgtgatattt ttgaattaga
     1921 aggtaattca aaattagttt atagtcttct gtgaatccaa taaaaagtac agggtacaag
     1981 aattgttagt aaaattttta aagattttat ggataaatat aaatagtttt actcattgtc
     2041 taaaacttta aaataccatt aattacctta aatattttaa taggaggaca tatgaattta
     2101 aataaagtta aatttactga agaacatgct attgaagtta ctaattggaa atatgaaggt
     2161 aaatattcaa tatacaatct acctccatgg gaggaaatta aaaagaagaa tttttcacta
     2221 gcaaaagaag ataaaagaaa aaattttata agttttataa atgacaataa agaattgata
     2281 ggttttataa acttattaga tgaaggaagc tctgtattct ttggtattgg tatgaaaccg
     2341 aattattgtg gaatgggaat tggtaaagaa ataattggcc taggattaca agaatgtaga
     2401 aataaatata gtactaaacc aattgtatta aatgtaagaa cctggaacat gagagctgta
     2461 aaatgttatg agtctcaagg atttaaaata gttgagacta aggtacagaa aacgcattta
     2521 ggagatggag aattttttgt tatgaggtat caatagttta taacaatagt cttattgtta
     2581 atacaacaat taaaatgaaa aaatacatat gtgtaggcca tagcaataag tgtctttgaa
     2641 atataaattt gagagctttt gataaaattt ggtatacata tatattttat actacggata
     2701 tataaatcta tctacatata taaagagatt gtaagtaggt ttgtgtatat aaaaatagat
     2761 ttatatttca taaatgtact ttttatttgt attaccttta ttttatctaa gctctcattt
     2821 tattatctaa tcttcctttt tactggtttt attaatagag atgcaattat caacacaaca
     2881 cttccaagta tcagccaata tttctcatca aaactacctt gactcataga cattataaaa
     2941 aattttccaa catacttgcc tttagtcatc atatttaaaa ttaaatatcc tatagatatc
     3001 atataaccta tgattgtatt attaaataaa tttgagaaaa acattcctaa acttcctata
     3061 aacatagctg atgcgaaact tccaataata tactcaacta ctggaaaatt acatccaaga
     3121 tatattaaaa catttacaaa acctatgatt aataccacta tacctataat tgacatcagt
     3181 atcctaatta attgtacaat aacttgagat aatgcttttg aagatactat ctcatcaatt
     3241 tcttttgatt gctctagcga aaatacaggt accaacatta ctatccctat tattgcaaca
     3301 taattttcta aacatatagc tgttgaaatt ctgtctaaat tagctactcc ttgtattaga
     3361 ggaatcatac atagcaatat aattgaacat attaaattag ttaaagtgct taatttgaag
     3421 ttttctttta ttagaactaa atagttttga aaactttgcc caaacatcaa gattccccct
     3481 tcttttttgc tcatataaga atatagctaa caaaacaagc accaatgctt ccaagcttat
     3541 cacaattcta ttaatcataa tttgattgaa gttttgtata ataatatctc catttaatac
     3601 actattatgt cttatcatta gtcccattgt tgaataatca cctttgagtc ctgtagaatt
     3661 tatactgtaa aaccaccaaa ttaattgcac aataattgct actggtgtat ctgttaatat
     3721 agtaatactc attcctacag ctgtaactat cataagagtt ggtagtatcc accataatgt
     3781 atattttata aatgctaagt tatcaataga tataccttta tctttagcaa agaaaatcaa
     3841 aggaagtaaa tctttcatac ctaaaattaa tacaggcaat atcatcataa agacaagtgc
     3901 aaaatatctt gataaaacta ttttgcatga agatacttgt tttgaataaa taatctctct
     3961 cattcttgaa cgcttatctt taagtgacat aaaaactgcc acaaatactg gaaatatact
     4021 taaaactata cctatataat cgcaaaaaat tcttgcatac ccattactaa ctttatcttt
     4081 ttgtattgta tagttgtaat tcttcatagc ttcatcatag gtaactggaa ctactccaaa
     4141 tctttgtaac ttgtcaaaag catatgcaga ccctcctcct aaaatgtcgt ctactctttg
     4201 cattatttct tcaaatcttt caagagatac tttagaataa tctattttta tactttcatc
     4261 agttttccaa agtgaattta tttcgctaat acttttacca gttatttctt ctatatattt
     4321 ttctatggtc ttcgttttat tttggtctaa ttttatatac ttaactaccc ctgttggata
     4381 cgttgtatat gtgttttttt gatattctct aattaggttt atagttgcat ttggaatgac
     4441 tttttctgtt ttcatagatt ctttttgacc atatggatat tgattgtgta cagagtaata
     4501 ttcctcaata ttctctactt tttgtggagc aactaatgga tttccatcat taggaatatt
     4561 tttacttaca tcatttgaaa ctgaacttcc aagttgagaa atatagaaaa aaactaaaac
     4621 tacaataaaa atataatata tcatactctt taatatattt ttacattctt ttataaatag
     4681 attcatatat tatctcctcc cattataagc atatagccat cttcaatagt tggattacaa
     4741 tttactgctg tctcaaaagg tttatcatct gatataatcc taagtatagc ctttccacca
     4801 tgagtcaaca ttcctataac tataaatcta ctttttatat tttcaatatc ttttttatct
     4861 acttcaatac tatatacttt cccttcagct agttttgcta atgcttctgt tgagccattg
     4921 tatatgactt ttccactatt caatatagct acgttttcac atgtagcttc tatatctcca
     4981 acaatatgtg tagataaaat aacgattcta tcatttgcaa tttcgcatag taaatttcta
     5041 aatcttatac gttcttctgg gtcaagacct gctgtcggct catctactat taaaactcta
     5101 ggattatgaa ttaatgcttg tgctatccca agccttctat tcatacctcc agataaagat
     5161 tttactttca cattttgata attagtaagg tttactttgc ttaataactt tgctataatt
     5221 tttcttcttt ttcttagtgg tatatccgat aaaactgcta aataatccat tgctttatat
     5281 actgtcatgt taggatacat tgaaaaatct tgtggcaaat aaccaaccat atttctaatt
     5341 tccttagtat tctcaatagg aactccacac atatgtattg aaccttctgt ttttgataat
     5401 aaagtggcta aaattctcat aaatgtggtt tttcctgctc catttcttcc taaaagacca
     5461 aacataccac tgtcgatttc aagatttata ttatcaagtg cttgtttttt cccaaatctt
     5521 ttgctaagat tggatactgc tattttcatt tccatatata aatacacccc cagaaacaat
     5581 tttatagaat tttattttta taaattttta ctgt
//
LOCUS       DAEOSP010000210         5571 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.214, whole genome shotgun sequence.
ACCESSION   DAEOSP010000210 DAEOSP010000000
VERSION     DAEOSP010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.214"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            208..1020
                     /locus_tag="KRH54_003140"
     CDS             208..1020
                     /locus_tag="KRH54_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBG8580774.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            complement(1109..1300)
                     /locus_tag="KRH54_003141"
     CDS             complement(1109..1300)
                     /locus_tag="KRH54_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580775.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            1571..1810
                     /locus_tag="KRH54_003142"
     CDS             1571..1810
                     /locus_tag="KRH54_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8580776.1"
                     /translation="MNYNLIKVIFSLFKKKSGYTFTNFLNKVRVEKSKKYILKKDLSI
                     LDVAVLIGFNSQNYYSIIFKKFNNFTPIEYKNIYN"
     gene            2119..3738
                     /locus_tag="KRH54_003143"
     CDS             2119..3738
                     /locus_tag="KRH54_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBG8580777.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            4112..4306
                     /locus_tag="KRH54_003144"
     CDS             4112..4306
                     /locus_tag="KRH54_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG8580778.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
     gene            complement(4865..5188)
                     /locus_tag="KRH54_003145"
     CDS             complement(4865..5188)
                     /locus_tag="KRH54_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580779.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
     gene            complement(5233..5487)
                     /locus_tag="KRH54_003146"
     CDS             complement(5233..5487)
                     /locus_tag="KRH54_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580780.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
BASE COUNT         2062 a          685 c          707 g         2117 t
ORIGIN      
        1 ctaagttcta taactaaatt gaaataatca ctttttcaag aatgttataa tttttttatt
       61 gtattctttt tgctatagtc tatgaataaa tcaatagaaa atcttctaag ttgcttttaa
      121 ataatgactt tatggttcaa ttgtactaaa atagtatatg taggttacaa ataatctaat
      181 aaatattaaa gtttggaggt aatagatatg gcagattgta attcatgtcc atcaaaggga
      241 aattgtaata gtcaatcaaa ttgttctatt gaaaataacc ctaacaataa atttggaaaa
      301 ataattggtg ttatgagtgg gaagggtgga gttggaaaat caactgtaac tgctttactt
      361 gctaataagc taaacaaaat gggatataag gttggtatat tagactcaga tataactgga
      421 ccaagtattc ctagacttat gggtgtaaaa aatgtaaaag cttattctga tggctcttat
      481 atttaccctg tagaaaactc aaataacata aaagtaatgt ctataaacct tatgatagat
      541 gatgaaaatg aacctgttgt ttggagagga cctttacttg gaggggttgt aaagcaattt
      601 tatactgatg tactttggga agaattagac tatttattaa tagatatgcc tcctggtact
      661 ggagatgttg ccttaacagt tatgcaatct attcctataa gtggaatagt tatggtttca
      721 gtacctcaag atttggtatc tatgattgta tcaaaagctg ttaatatggc taaaaaaatg
      781 aacataaatg tccttggagt tattgaaaat atgagttata tccaatgtcc tgattgttct
      841 aaaaaaatca agttatttga aggagaatct actgaaaagt ttttagatga tttagactta
      901 gagcttttag gagaacttcc tatgactaaa gaaattatag atattactca taatggagtt
      961 acagaaatta gtgatgattt agattcaata ttgactaatg ttgttgaaaa aattaaataa
     1021 ataaaaattt tataatttta tatttatcaa aaaaagaggc ttgtatttat tttataatca
     1081 tacaaataaa tataaacctc ttttatgttt atctatgttt taaatacaca aaatattctt
     1141 ctacttcatc tccatttttt acttttacta aatattcatt ataatgagca ttatatggct
     1201 gtgtcatttt gcattctatt acattttcta tgtcttctaa ttctaaaagt tctacatcct
     1261 ttaaaaattt tctttctatg agttccatta atctttccat aatatatcct cacttcccct
     1321 ataattaaga taaactatat atcctcttaa tgcagagttt atctgtttat tatattatat
     1381 ccaatatatt acagtttcta acatcattca taaattattt taacaaatag agattgattt
     1441 tttaataaat atgccttgat aaaatgaaac atagaccaac tcttagtttt atgtaaagaa
     1501 aacttaaaga aaactataaa ttatattcac aaaaattact cagaagatat aacagtaagt
     1561 aatatatgta atgaattaca acttaataaa agttattttt tctttgttta agaaaaaaag
     1621 tggatatact tttactaatt ttttaaataa agttagagtt gaaaaaagca aaaagtatat
     1681 attaaaaaaa gacttgtcta ttttagacgt tgctgtacta attggattca atagtcaaaa
     1741 ttactatagt ataattttta aaaagtttaa taactttaca ccaattgaat ataaaaatat
     1801 ttataactaa aaaatatcat tctaaaagga tgttattttt ttacatattt tttacaatta
     1861 tagttaattc tttgttaaat tgtttgtaca taaaatacta aaaatctaag ttcaagtatt
     1921 taaattgcta tatatcataa attttctaaa attaacaaca acttaaaaat ttaacattct
     1981 cttaacgtat acttaacaaa acattaatat ttttattgac aaaatttgtt attctatatt
     2041 atggaaacaa taaattttag ttaataaagt ttataattca gataaaaaaa ttattaattt
     2101 cttaagggag gaaatatcgt gaatatagta attagcctta tcggtggact tggacttttt
     2161 ctttacggaa tgagtttaat gggagaaggt ttacaaaaat cagcaggaga caaattaaaa
     2221 aaaattattg aactcttaac aagtaatgtt gttatgggtg tactggtagg tacagtcgtt
     2281 acaggtatta tacaaagttc aagtgctact actgttatgg ttgtaggatt tgtaaatgca
     2341 ggtataatga atctaagtca agctattggt gtaataatgg gggcaaatat aggtactaca
     2401 gttactgctc aattagtttc atttaatctt gaaggaattg caccaattgc tttaggtata
     2461 ggtattttat tctatttatt tacatcaaat caaaaaacaa agcatttatc tgagatactt
     2521 ataggtttcg gtatattatt tacaggaatg gagtttatga aagatgcagt agctccactt
     2581 gctgaatata aagcattcac tgatgcactt ttatatttta gtaaaaatcc tgtattaggt
     2641 atattggcag gatttgccat aactggtatt gttcaaagtt caagtgcatc tatgggtatg
     2701 ttaatagcac tagcttctca aggaatactt cctctatcat ctgctttacc tattctttat
     2761 ggagataata taggtacttg tgttacttca cttttatcaa gtgttggtgc aagtagaaat
     2821 gctcgacgtg cagctgtaat gcatttaagt tttaatgtaa ttggtacaat aatatttatg
     2881 cttgttttaa ataaacctat atcagccata gttactcatt ttgacccaac tgatacagct
     2941 agacaaatag caaatgcaca tactttattt aatcttacaa atgttataat attattacca
     3001 ttttctaaat acatagttaa attagcaaat agacttatac ctattaaaga aactgaatca
     3061 gaaatagtaa ataatactaa gtacttagat gaaaggatgt ttagcactcc atctatagca
     3121 cttggtaata ctgtacaaga agttgtaaga atgggtcata aagctacaaa ctctttggaa
     3181 cattcaattg caggattttt aaataagagc aatgaagata ttaataaagc ttttgagtca
     3241 gaaaaagttg tcaataagtt acagaaagat atattgaact atttgttaaa actttctaaa
     3301 gaacctctta gagatgatga acgctttaga acagatttgc tttttaatac tgtaaatgac
     3361 attgaaagag tctctgacca tgcagaaaat atagctgaat tggccatgag tgttaaggaa
     3421 atgaacatta gtttttctga tagtgcgata agagaaatat acgaaatata caataagaca
     3481 attactaatt ttaaggatgc cttaatagtt ttagatgtta aagattttga gttagcaaat
     3541 aaagttttag aagttgaaaa tgaagtaaat tatttagaaa aaacttttag aaattcacat
     3601 atgattagac taaataatgg aagttgtaca attgatgctg gtgtacttta tcttgactta
     3661 cttactaatc tagaaagaat ttctgaccac tcaacaaata tagtaaaaca agtactaaaa
     3721 cttaaacaaa aaatatagtg attattttta tatataatct taatcaatga gaagtaagaa
     3781 gctatagata atgcttatat catttatggc ttcttatttt ttatgtaata ctcaaatttt
     3841 tatagtattc tcttattatt aaacagtttc tgcctagaat attccatata tatatgactt
     3901 ctttcataaa acttgaactg cccataaata aagtcacaag tatacataac tattttttaa
     3961 tcaattataa ccaatatttg ttttatttat cttcatttta attcttcatt caataatgta
     4021 ataatattat tttttatttg gatactatta tatcatctaa atatgttatt atatatttgg
     4081 atattttcat attaatagga gggattctac aatgataaat tatatggtag aaattatata
     4141 tagcgaaaaa ataagcgaag tagaaaacat gttgaaaatt gaaaatatac aatatgaatt
     4201 tacttctcgt gatggcttta tattttttgg tttttatgat atgacagacg catatagatt
     4261 tgagacttta tgttcagatg aaaaaattcg atttaaaaga atataataga taaaaaagta
     4321 catatttcta aactatttgt ttttcaaatt taggttgtgt tttaatgtag tgttgatatt
     4381 ataaacaatt ttaataataa aaaagtaggg acattatatt aatatgctcc tactttttac
     4441 tcatacttat taatattatt tatcaaaatt accctacaaa ctggaattta tcattctttc
     4501 ataattttaa actatcatat ttaaaatata taaaattcta catgattttt gtataaacat
     4561 aatatcaacc attatccaat ttaaaaatat tttttagtaa tttccatttc attttctctt
     4621 aacatattaa ttaaactttt atatgtaaat ggtgtattta catattattt aatatccaac
     4681 aattatataa agaaatgtgc tctttgcctt ataaagaatt ataaagccaa ttcgttttat
     4741 tatattattt ttctttatac cttaaaaact ttaccttcaa attcttattt atctatatat
     4801 tataaaaatg ttcttaacct cttttacgag attttcttga catattatca gttagataaa
     4861 ataattaaat aatataatca gcaagtatgt tttttactct tttaagtaaa tttccacctg
     4921 caccccaatc aatttttaaa attccaattg cactgcctgt tgaagtagct ttaataactg
     4981 tttttcctgt ggtattttca taaactacta ttaatcctgc atcactatta tttcttgcat
     5041 aatacttctc aaaagttgta attagtataa atttatcatc tcctaaatta taagaatcac
     5101 taaaaactaa atctgcatca agattttctt ttatcatttt ttgtgcctct aagggtgtaa
     5161 tttttacagt aaatatttct gttgccattt tttatctcct cataagaatt tattttagac
     5221 aaaacataat ctctagtatt ttctgatata tttatcaaac aatatcccta ttcctcctaa
     5281 cagagctaat atagaaccaa aatttctata ttgagaaata gattggtcta gataaatatt
     5341 atattggtca gtatccatga ttccaccatt ttgttgaata atatgctctg cactaaattc
     5401 acctaaacct atacaacaga ataatactac tactccaatt acaaaagcaa atatacatat
     5461 tgttaattta aaatccttat tttccatagt tatccctctc ataagctaaa tttatttgat
     5521 tatttttact agattttata ttactcttat tatacaatac aacatatatt t
//
LOCUS       DAEOSP010000211         5513 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.215, whole genome shotgun sequence.
ACCESSION   DAEOSP010000211 DAEOSP010000000
VERSION     DAEOSP010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.215"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            137..1342
                     /locus_tag="KRH54_003147"
     CDS             137..1342
                     /locus_tag="KRH54_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBG8580781.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKG
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1413..1634)
                     /locus_tag="KRH54_003148"
     CDS             complement(1413..1634)
                     /locus_tag="KRH54_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580782.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(1775..2374)
                     /gene="recR"
                     /locus_tag="KRH54_003149"
     CDS             complement(1775..2374)
                     /gene="recR"
                     /locus_tag="KRH54_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBG8580783.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2387..2737)
                     /locus_tag="KRH54_003150"
     CDS             complement(2387..2737)
                     /locus_tag="KRH54_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBG8580784.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(2796..4433)
                     /gene="dnaX"
                     /locus_tag="KRH54_003151"
     CDS             complement(2796..4433)
                     /gene="dnaX"
                     /locus_tag="KRH54_003151"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBG8580785.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4510..4774)
                     /gene="ffs"
                     /locus_tag="KRH54_003152"
     ncRNA           complement(4510..4774)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KRH54_003152"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4812..4903)
                     /locus_tag="KRH54_003153"
     tRNA            complement(4812..4903)
                     /locus_tag="KRH54_003153"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4867..4869),aa:Ser,seq:gga)
     gene            complement(4904..5359)
                     /locus_tag="KRH54_003154"
     CDS             complement(4904..5359)
                     /locus_tag="KRH54_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBG8580786.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
BASE COUNT         1754 a          980 c          637 g         2142 t
ORIGIN      
        1 ctttcacttg taatttttgc aatcttgtat tagattaata ttaaaaataa tttatcaata
       61 accttattca caaagacagt ttttatatat caaaatataa tataaatact atgctacatt
      121 ttaggaggat tttattatga gcaaaaaagt attaataatg tctgcttcca ctggtggtgg
      181 gcataatagg gctgctctag ctattaaaga agaacttact tccaaaacac ttgatggaga
      241 gccaattgaa tgtgaaataa tagatagttt gaaattagtt aataatacaa tggataaaat
      301 aatttcaaga ggatacgaaa aatctgctat atatactccc aaagcctatg gaagtgtata
      361 tagattatct gaaacaaatc ttttatcaaa aaatgaattt aaagataatc tactaataac
      421 attcatggct aaaaaattta aaaaacttat tagaagcgaa aaaccagact tgattatagg
      481 aacgcatcct tttccaatga ttgctctaag tacattgaaa aaaaacttta atttacataa
      541 taatgaatca aatgcttaca cagagcattt ttataaacat tatacaaata ccattaatgt
      601 tcctccgctt atttctgtat taaccgatta tactactcat tctacatgga tacaaaatga
      661 aattgattac tatattgttg gtcatgaata tgtaaaagaa ttattggtgt ttgatggagt
      721 tgaaccgagt aaaattagaa catttggaat ccctgttgaa aaatcttttc tctctcacag
      781 agataaaggt attgttcttt ctgagttgaa tttatctcct gataaattaa ctgttttact
      841 tatggggggt agctttgggg ctggaaatat aaaagagaca cttgatgaat tactggatac
      901 tgatagagac tttcaaatac ttgtaattac tggtaagaat gaaagcctaa aagaaaaaat
      961 cgaaaaaaaa cttatgtctc gttatcatga taaaaatgta tgtgttcttg gatacactga
     1021 taagatgaat gatattttag cttctataga tgtattagta agcaaacctg gtggacttac
     1081 tacaactgag gctcttttaa aagatgtacc tatgatagtt ccttattata taccaggtca
     1141 ggaagaagag aatcttgatt tcttatctaa ttgtggtgca gcacttagaa ctacaaaaaa
     1201 atataacttg tcaatattac taaaagtttt aatagatgac cctagcagat tagaaatgct
     1261 aaaaagaaat attaaatcaa taagaaagtc aaattcagcc caaaatatag ctaatttaat
     1321 tttaaatata atttctggtt gataaaataa ataaaatccc tatagtatat atattatcta
     1381 ttagggattt tttattttct aattatttaa tattaaattg gttcataaaa gtcttcttca
     1441 taatcatact gataattttt ataattattt ctaaattcag catttttatt tcgtacattt
     1501 tttttagttt ttttcttatt agaaaaactt atatcctcaa aatcatcata tttaggttta
     1561 tcttttgatt tcattttttt aactcttttt tctttcattg cattatgttc ttctaagttc
     1621 ataattggtt tcatgttgca cccctatcaa aatttttaaa tgatttatac aacaaattat
     1681 acaatacaga agaaatttat tcaataaatc taaatcaaaa ttaatttgaa tttaactcaa
     1741 aaaattacaa aaatcgaggt attaacctcg atttttatat ttctcttctg ccttctagtg
     1801 cttttgatat tgtgacctca tctgcatact ctaaatcgcc tccaacagga agaccatgag
     1861 ctatccgagt gactttgatt cccattggtt ttactaacct agcaatatac atggctgtag
     1921 cttcaccttc aatagtagga tttgttgcca ttattatttc tttgacttcc tgattgccaa
     1981 gtctttggat aagttccttt atttttaaca tatctggtcc aattccatcc attggagaaa
     2041 ttactccatt taatacatgg tactgtcctt taaactctct cgttctctcc atagctgcta
     2101 catctcttgg atcttccaca acacatataa cacttgaatc tctactttta tttgaacaca
     2161 tgctacatgg gtctgtatct gttatattac aacaaattga gcaatatctt atttctcttt
     2221 ttgcatctat aatcgcttta gataaagctt ccacatcatt agtattcata ttaataatat
     2281 gaaaagccaa tctttgagca gtctttcttc ctactccagg aagctttgaa aattcctcta
     2341 taagtctcgt tatgggacct gtataaactt gcataaatac cacctactag aataacccag
     2401 gtatattcat acctccagtt accttactca tttgacttgc ttgtatatca tcaatatttc
     2461 tcaatgcttg atttactgca cttaaaacta aatcttgtag catttctata tcatctggat
     2521 caactacttc tggcttgata gttatatcta taacttcttt tttaccatta acttttacag
     2581 taactgctcc tccaccaact gaagcttcta cttctttact ttctaactct tgttgagctt
     2641 tttgcatatt ttcttgcatt ttttgagcct gctttaacaa gttgttcata tttccaccac
     2701 caggcatcat tccgcctcca aaaccttttt tagccataaa cactattcct cctaaactta
     2761 ttttcatttt attattatat cataatagta ttttattatt tactctcatt ttcatcaata
     2821 ctatctttta tttctaatat ctcttcactc actatgttct ttaatatttc ctcaccaatg
     2881 tcctttttct ctttaacttc aagatttata tctttaactt ctgatgtcag tactattttt
     2941 acattaaaac ttctatttaa aacatctctt attgtttttt ctaaataatt tattgtatct
     3001 tgagagctca gtctagtttt tgcaaaatca aatttatcat caaatataac atacaacata
     3061 ttagaatata cattaaaact ttttacttca cttaagagtg cgtatattgg cattttttta
     3121 tcttttttaa tttgtttaag tatattcttc caagatgact ctataagtat aacgtcctca
     3181 ttttttacat cttcataaac tacattttct tgttttacat ctatttctct atcgacctca
     3241 tactcttttt ctttattatt cccaattttt tcagacttaa agttccccag ttcaatcatt
     3301 ttttctaagt tctcaactct ttttataaga gcttctttac tttcatcaaa cattggttga
     3361 gcaattttca ttatggttat ttccactaaa acccttggat ttgaagatga ctttatatca
     3421 tcctgagtta ttgataatat atttaaaatc cttattaaat catttatatc tatattttta
     3481 gactgaattt taagttgttc tatagtttct tctggtaatg aaattatttc atccaattct
     3541 gatgaaacct tacataccat taaatttcta aaatgatcaa ttaagtcatt tataagattt
     3601 cttatatctt tgccccataa gacaaattca tttaatatct caagagactt tttagtgtcc
     3661 tgctctacta tacattgaga caattcaaat aattgttcta tatttacgct tcccattaat
     3721 tcaacaacat ctttataatc tatcttactt tctccaaaag acatacattg gtctaatata
     3781 ctaagtgcat ctctaagagc tccttgagaa tttctagcta ttaaattaag agctttatca
     3841 tctatatcaa ttccttcatc ttcacaaatc tttttcattc taagggtcat atctttaact
     3901 gtaactcttt taaaatcgta tctttggcat cttgataaaa tggttgcagg tattttgtga
     3961 ggctctgtag ttgctaatat aaaaattaca tatgatggag gttcctctaa ggtctttaat
     4021 aatgcattaa aagctccttg agacaacata tgaacttcat ctattatata tactttatat
     4081 ttagcattgg ctggagaata tttcacactt tctctaagct ctcttatatc atctacacta
     4141 ttatttgaag ctgcatctat ttctacaaca tccattgtat tatcttttaa tacagattcg
     4201 catatctcac actcattaca cggttcttca tttattttat tcaaacagtt tactgctctt
     4261 gaaaaaatct ttgcagtaga tgttttacca gtacctcttg ttccacaaaa caaataagca
     4321 tgtcctatat tatcactata tatttgattc tttaaagtct taataatatg atcttgacca
     4381 ataacatctt caaatttttg tggtctatat gctctatata atgctttatg catcttttta
     4441 cctctcttta gcaaaattaa taaaaaaatc cctcattatc taagggatta gtcttttata
     4501 tattaaatgc tgtacaccat ctatcgactt aattccatag gcgttaccaa aacagttagc
     4561 tcaacttagg cacccccacg gcacacaaaa gtgatcactt accgctgctt ccttccggat
     4621 ctgacggggt tcatgagctt tcattgcgca ggacctaagc atcaacacca cttatttagg
     4681 gcggccctac agaaaataag cctttagatg gaattcaatc ctgctatagc ggattgcagg
     4741 ttacagggca ccgctacctc cccatctagt acagcaaaat tacaaccata tttaattact
     4801 ttcattgttt ctggcggaga gagtgggatt tgaacccacg atacagtttt acccatatac
     4861 tcgctttcca ggcgagcgcc ttcgaccact cgaccatctc tccttaatct ctttttctca
     4921 atgccttaaa aaattcttgc aatatgatcg agcattcttc ttgcaaaaca tcaaaagcta
     4981 cttttacatt attattatta aaataatcta atttaaactc atgctgtttt tctatatatg
     5041 aattttttac atgtcttgtc cctattataa gcttttttat tctagaatta actattgcgc
     5101 cactacacat tatgcaaggt tccattgtaa catataaatc acaatcagtc aacctccaac
     5161 cacctaattt ttctgatgct tgttttatcg ctaaaatttc tgcatgtgcc gtagagtctt
     5221 ttaacgtttc tgtaaggtta tgagctctag ctattatttg attatcttta actataatag
     5281 cacctattgg tgtttctttt ttattatatg ctttatatgc ttcttttaat gcttctttca
     5341 tataaaatga actttccata attcctccca aaacttataa caaacttatc ttatcataat
     5401 tcattttata attcaactta attttatttt gtttatttaa ttattactat atatagctat
     5461 atttttttaa aattagttct aaaataattt ttagatattc atacaagagt gtt
//
LOCUS       DAEOSP010000212         5501 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.216, whole genome shotgun sequence.
ACCESSION   DAEOSP010000212 DAEOSP010000000
VERSION     DAEOSP010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.216"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            471..1394
                     /locus_tag="KRH54_003155"
     CDS             471..1394
                     /locus_tag="KRH54_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBG8580787.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1369..2196
                     /gene="panB"
                     /locus_tag="KRH54_003156"
     CDS             1369..2196
                     /gene="panB"
                     /locus_tag="KRH54_003156"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBG8580788.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            2215..3063
                     /locus_tag="KRH54_003157"
     CDS             2215..3063
                     /locus_tag="KRH54_003157"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBG8580789.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAI
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELIIAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
     gene            3474..3656
                     /locus_tag="KRH54_003158"
     CDS             3474..3656
                     /locus_tag="KRH54_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580790.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            4488..5402
                     /locus_tag="KRH54_003159"
     CDS             4488..5402
                     /locus_tag="KRH54_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBG8580791.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
BASE COUNT         2191 a          655 c          758 g         1897 t
ORIGIN      
        1 aaaaaaaatt aatattacat aatacatctc tctcttatat taaattttaa cttggtacta
       61 atcttgacaa attgtccaca aaaataaatt atagatttta ttattgtttt aaatttttat
      121 aaaaaaatca gtattattga tactattgaa gttaaaaagc attaatttct aaaaacatca
      181 taatttttct aatagactta atcactttta atatatatag taaaacaaaa aacttatatt
      241 ttataaatat cataattttt ttactgatat tttattatat aaattttata gaattaattg
      301 tcaatttttt atgaaaatgg taagatattt atggatggta tcttgttata agaactatac
      361 tttaaatgtt tttaattatt ttgtcttatg taactcatat cctcaggaat gagattagaa
      421 attaagatgt tgtatcacaa agtggaggtg atactattaa aataggcttt attggttctg
      481 gaaaagttgg aacatctttg gcaaattatt ttttatcaaa aggttattgt gtttcaggct
      541 tttatggaag gaatcaatta tctttattag aatcaacaaa tttaacaaaa acaaagattt
      601 acagcaattt gaaagatata atttatgaaa acgatatatt atttattaca acatcagatg
      661 attctattga aattatagac aaaaaacttt ccaaatttaa tttaaaacat aaatacattt
      721 gccacacaag tggctcttta aaatctagta tattgttctg ttcaaaaaaa gcaggtgcat
      781 taatttattc tatccatcct atatttgcat tttccaataa aaataccaac ataaaaaaaa
      841 tgaaagacat ttgtttttcc atagaaggtg ataatgaaca agatgattta gttattcaaa
      901 attttataga tgatttggga aaccaatttt ttataagaga taaggatact tcatccactt
      961 atcatttagc taatgtttta gtttcaaatt tagtattatc tttactagat ataggagtaa
     1021 gttactttat taaattaggt ttatctgaag aggaatcttt aaaagctatc agtcctttag
     1081 taaaaaaaaa tatagaaaat atacttgata atggctttac taaagctctt actggtcctg
     1141 tttctcgtgg agatataaca ccaataagaa aacatctatc tgtattggat aaaaaagatg
     1201 aggatattta caaaatacta tccttaaacc tactaaaaat aattgcctta ggaaatgaca
     1261 attcattaaa aggtataaaa aatattacta tagaaaatgc aatcgaaaat ttaataagta
     1321 aatctgaaaa atacaaagaa atttacaaac ttttaggagg aaaaaacgat gaaaaatacc
     1381 atacagactt ttaagaatgc taaatatgaa ggaaaaaaac tatctatgct tactgcttat
     1441 gactactcaa ttgcaaaaat aatggatgag tgtgatataa atggtatttt aataggagat
     1501 tcattaggaa tggtcataaa aggtgaagaa aacactctat ctgttaccat agacgaaatt
     1561 atatatcata caaaagctgt aaaaaatggt gtaaaaaatg ccttaatcgt aagtgatatg
     1621 ccttttctat cttaccatgt ttctatagaa gatgcagtta aaaacgctgg tagacttata
     1681 aaagaaggag gagctcatgc tgtaaagtta gaaggtggct caaacgttat aaagcaaata
     1741 gaaagcattg taaatgctca aatacctgta atggggcatt taggattaac tcctcaatca
     1801 gtaaattcat ttggtgggtt taaggttcaa ggaaatacca gtgaaacagc aagacaactt
     1861 attgaagatg ctaaattaat agaaaaagca ggtgcattct ctattgtcct tgaaggagtg
     1921 cctacgaaaa tagctgagat ggtaactaat tctatttcaa ttcctacaat aggtataggg
     1981 gcaggaatta actgtgatgg acaaatttta gtttatcaag atatgttggg aatgtttgga
     2041 gattttgttc ctaagtttgt aaagcagtat gcaaatatag gagatataat gaaagattca
     2101 attaaaaatt acatcctaga agttaataca ggtgcatttc cacaagaaaa acacagcttt
     2161 tcaataaatg aatctgagtt agaaaaatta tactaatatt aaggatggta caaaatgctt
     2221 gtaaaagaaa ttaaattact aagaaatata atcaaagatt ggagaaaaca tggctattct
     2281 ataggccttg ttacaacaat gggattttta catgaaggac accaaagtct tataaaaaaa
     2341 gctataaaag aaaatgataa agtagtagtg agtgtatttg taaatccaac tcaatttgga
     2401 ccaaatgaag attttaatag ctatccaaga gatatagata aagattttaa atattgtatg
     2461 gatagtggag caacagtagt ctttaaccca tcaccagaag aaatgtattt aaaaggtaat
     2521 tgtactacca ttaatgtttc tggacttaca gattttcttt gtggagctaa aagacctgtt
     2581 cattttggag gtgtctgcct agttgtttca aagtttctaa atatagtaac acctgataaa
     2641 gcttatttcg gagagaagga tgcccaacaa ttagctgtaa ttaaaagaat ggtcaaggat
     2701 ttaaacatag atacagaaat aataggctgt cctataattc gtgaaaatga tggattagca
     2761 aaaagttcga gaaatacata tctctctgag gaagaacgaa aatctgctct aatattaaat
     2821 aagagcttaa gtttagcaaa agaagaatta gtaaaaggca atctaaatcc agagaatata
     2881 aaagaattaa taatagctaa aataaattct gaacacttag caaaaataga ctatgttgaa
     2941 attgtagata gtgagacatt acaacctgtt aagcaaatag aacattctat acttgtggca
     3001 attgctgtgt ttataggaaa aaccagacta attgataatt ttacatttga gttaaatata
     3061 tagtctataa ttttatataa tatatctaaa aaataataga atacctaaaa agtaataaat
     3121 tacctaaaaa ataataaatt gcttagaaaa taatagatta caataactaa aattttaaaa
     3181 ttaaattcct taaaagtatg attaattata attatataag tatattctta ttacacttat
     3241 attaatttat attaaatatc tactaactca aatattcata attaaacttt aaactggata
     3301 aattttcttt taagaatgaa agtttatcca gttttgcgat tataataacc agttttacat
     3361 atccacttgt aaaatatatc taagcaatat atcatactat atagatttat gagtgaaata
     3421 atatctgcta tttactgaat atttttattt ttcgtttttt agggggatta gttgtgttta
     3481 aagataagat tgatgaatgt gttcatataa tgactgcata tattgctagt ttaaaagaat
     3541 actattcatt catagaaact caaattggtg attttattaa aaaatatgga gaagatgttg
     3601 tagagttatg tctccataga gtgatgattc tactatgcga atgtggtttg gcataaaatt
     3661 aaatttatta aaatgggtaa gaagaatcct atataatatt cttcttaccc attttaatat
     3721 atcaatttca tagcttatcg tttaaaatat tttacatcca ttactagaca actttacatt
     3781 ctctttttaa ttctagtata tttaaatcat tttagttgct ccttatttat tcttattact
     3841 tgttttattt tataaataaa ataattcttt ttaataatgt tttttgttga tattcctatc
     3901 acacatccaa ttatttatca ttgtcttgaa attgtttgca tacttaattt aatccaattg
     3961 aaacactcca gcatagaatc ccctgtaaaa tagaagttgg attaaatcct aacatctata
     4021 cagaaaatac tactccaagc acaagcaata taatggaata tatttatcac ttaatttttt
     4081 atacttttta aatcccatac ctaaaacatt aagtgcagct attagaatta ataattgttg
     4141 tggtataaaa ttatgcttgc ttcatattta ttcatttttt cctataaaag ttcttgtttt
     4201 acaaaaaaca tttgttttgt atcaaaacaa tacttataaa ttacataatt ctcataaaaa
     4261 tttctaatat tacttgtact cttaataaat aatagccaac aagcacctta aatcattata
     4321 tctatatcta ataagaaaca cagtaagata attttttcgc ccataactta ataaaaactg
     4381 ttgatattag tagcaattga atttagttaa tttatttata atatacataa atttaaatat
     4441 tacgaataaa gtattataaa taggatactt tctaaaggga ggaaattatg atagataatc
     4501 aaaaatatgt tattttatca ctagaattac atttattttt ttcaagaatt atgaaagagc
     4561 atgctctttt tttagaagca ggattcacaa ataaaaatta taatcttgct atggaagctg
     4621 accattataa aaagcaattt gaagatttat tatcatacac tgttagtgct agtaatggta
     4681 taattagacc tgatatatta tattcagaag agcttgtaac tactctcaca tcagttgcag
     4741 aacaaaaaac agaagagttt acagggatag aaataaacaa aaacatcact acaagagaat
     4801 taaatctaca aagtggtgta aacccacaag ttggtcaaga tttagtgaac tatgtagctc
     4861 aacttaactc tgatgcaata agattacttg atgggcttat taattttaaa gaaagagtct
     4921 tagatggtgt actatcatgt actatattta catcaaacta ccctctactt cttgaacata
     4981 taatacatga agcaaattta tatcgttctt atgtagttga ccttgaaaat aaaatagata
     5041 ttgagtcaaa aaacgctaaa gaaatagaat tattctggga ccatattatg atggaacatg
     5101 ctctgtttat gagaggatta ctagacccct cagaaggtga actaataaat acttcaaatg
     5161 attttgctat aaaatttaat gaattaattg aaaaaacaaa cgaaatgact gattctaata
     5221 tcaagaacat tacagaagaa actctaaatg aaactgttga gtttaaagat tttaaagaag
     5281 caggagcatc aggaatagaa cagtgtaaga taaaatctat aatattacca cttttagcag
     5341 accatgtttt aagagaggca aatcattata ttagaatatt ggagagttat aaaaacatgt
     5401 aattaaaaat tacttttaaa ataatctttg catatttaga taaaataaag gtacagttaa
     5461 ctgtaccttt attttttata aacttataaa atttaaacaa g
//
LOCUS       DAEOSP010000213         5449 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.217, whole genome shotgun sequence.
ACCESSION   DAEOSP010000213 DAEOSP010000000
VERSION     DAEOSP010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.217"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            133..513
                     /locus_tag="KRH54_003160"
     CDS             133..513
                     /locus_tag="KRH54_003160"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBG8580792.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            528..989
                     /locus_tag="KRH54_003161"
     CDS             528..989
                     /locus_tag="KRH54_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBG8580793.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            1010..1600
                     /locus_tag="KRH54_003162"
     CDS             1010..1600
                     /locus_tag="KRH54_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580794.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            1615..2772
                     /gene="alr"
                     /locus_tag="KRH54_003163"
     CDS             1615..2772
                     /gene="alr"
                     /locus_tag="KRH54_003163"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBG8580795.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            2805..3092
                     /locus_tag="KRH54_003164"
     CDS             2805..3092
                     /locus_tag="KRH54_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBG8580796.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            3061..3444
                     /locus_tag="KRH54_003165"
     CDS             3061..3444
                     /locus_tag="KRH54_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBG8580797.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            3615..4442
                     /locus_tag="KRH54_003166"
     CDS             3615..4442
                     /locus_tag="KRH54_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBG8580798.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            4435..5376
                     /locus_tag="KRH54_003167"
     CDS             4435..5376
                     /locus_tag="KRH54_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBG8580799.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEVIIAKRM"
BASE COUNT         2174 a          569 c         1085 g         1621 t
ORIGIN      
        1 acagttttat tataatgatt attaaattaa gataagtaac ataaactaaa taataatgtg
       61 tattatcgat agtaaattta taaattcatt attaattatt gaaagaagta ttataaattt
      121 tctggaggaa caatgaatat atttgatatt ggcgtagata taattgaaat tgatagaata
      181 agaaaggctg tagacaaaaa taataggttt ttggagaaga tatttacaga tagagaaata
      241 gagtatttta attctaagaa ttttaaagca gaaagtatag caggtaattt cgcagccaag
      301 gaagctataa gtaaatctat aggtacagga attagattgt ttaattttaa agatatagaa
      361 gttctcagaa atgaaatggg aaaaccaata gtaaaaactt ataataatct agcaaaaatg
      421 tgcatagatt ataatgtttt agaaataaaa gtttcaatat cacattcaaa agattatgct
      481 atagcaaatg cgataaccat aattaaggac taaggagtga ttacattatg atggataaaa
      541 cagcaaaaga aataatgaca acagatgtaa tagtagcaaa acaagatgat agtatagcag
      601 atgtagctaa tatgctaatt gcagaaaaaa taggaggtct gccagttgta gattcagaga
      661 atagagttgt tggaataatt tctgaaacag atattcttaa aaaagaaaaa tatatagaag
      721 cacctctcta tataaatcta ttacaaggat tgattttctt agatgactta aaaaaagttg
      781 aaaaagatat aaaacaagta gctgcttata aggttggaga acttatgtct aaagatataa
      841 taaaggttca tgaagatgat aagtttgatg atgtagctaa tgtaatgatt aaaaaatcaa
      901 taaacagagt tcctgtagta gatgatgaca acaaacttaa aggtataatt tgtagatatg
      961 atataataaa agctttatac aatgaataaa ctgaagtagg aggtgtgatg tgagaaaaaa
     1021 gtggaccata gtatgtatta tgttcttggc actggttata attgtaatag gatgccaaaa
     1081 acgacagtcc acaaaagaag aagtatacaa agattttcaa aagcagattt ctgatatgaa
     1141 ttattacagt tgtaaagctg aagtagaagt agttggaaat aaaagtccac acaattatgt
     1201 tttaattcat acttataaaa aaaccgataa ctataaatta gaagtaattt ccccaaaaca
     1261 tttaaaaggg aaaagtatag aatatcaagg tgataaaata ttagttaaaa atccaaagat
     1321 tagtgatgta gtggagttac ctaatacagg aaaaaataat caatatcttt ttgtaggaga
     1381 ctttataaaa aactatcttc aaaatgagga gatgaaagta aagttatcca aaggacattt
     1441 ggttttagag acttttattc ctggggataa taaatatttt aacaagcaag tattatatgt
     1501 aaatgctgac acaaaaaatc ctgaaaaaat ggaagtgtta gataaagagg gagtgccaag
     1561 atttacagta aaatacaaag attttgaata cagaaactaa gggggactaa agacatgcaa
     1621 aaaataacag tgcctacatg ggcagagata aatctagata acttaagatt taacttaaat
     1681 aatattaaaa atttattaga agaagatatt aagatttgtg gagtaataaa agctgatgca
     1741 tatggacatg gtgcagtaga agttgcaaaa ttgctagaaa aagaaaaagt agattactta
     1801 gcagtagcaa gaactgctga aggaattgaa cttagacaaa atggcataac acttcctatt
     1861 ttgaacttgg gatatactcc agacgaagct tttgaagatt ctataaaaaa taaaataact
     1921 atgacagttt attctttaga aacagcacaa aagataaatg aaattgcaaa atctttagga
     1981 gaaaaagcct gtgttcatgt taaaatagac tcagggatga ctagaatagg tttccaacct
     2041 aatgaggagt cagtacagga aataatagaa ttaaataaat tagaatatat agatttagaa
     2101 ggtatgttta ctcattttgc tacagctgat gaagtaagta aagagtacac ttataaacaa
     2161 gctaataatt ataaatttat gtctgataaa ttagatgagg ctggtgtaaa aatagctata
     2221 aaacatgtat caaacagtgc agctattatg gattgccctg atttaagatt aaatatggta
     2281 agagcaggaa taatattata tggtcattat ccatctgatg atgtatttaa agatagatta
     2341 gaattaagac cagccatgaa attaaaatca aaaatcggac atataaaaca agttgaacca
     2401 ggtgtaggaa taagttatgg actaaaatac acaactacag gtaaagaaac aatagctaca
     2461 gttccaatag gatacgcaga tggatttact agaatccaaa aaaatccaaa ggttcttatt
     2521 aagggagaag tgtttgatgt agttggtaga atatgtatgg atcaaataat ggttagaatt
     2581 gacaaagata tagacataaa agttggagat gaggttatac tatttggaga aggcgaagtt
     2641 acagctgagc gtatagctaa agacttagga actataaact atgaagtgtt atgtatgata
     2701 tcaagaagag ttgaccgtgt ttatatggaa aataatgagc ttgtacaaat aaacagttat
     2761 ttgctaaaat aaaaccatga tattataatc tgggaggcga ttttgtgtac gataagagta
     2821 aagaaaaaat agaagttact ttacctgact ctctgatttc tgaagttgat agtatagttc
     2881 agatggagaa cagtaacaga gaagattttg caaaagctgc ttttcagttc tatataactc
     2941 agaaaaagaa aattcatatt aaagaatcta tgaaaacggg ttatagagaa atgggtcaaa
     3001 ttaatctgtc actagctgaa ttaggtatga cagttgaaga agtatcttct gatgataaaa
     3061 atgaagggaa gattgcccaa ggtgagtact aattgtgaaa taaagcgtgg tgatttatat
     3121 tacgctgatt taagtccagt tgttggttca gagcagggag gcgttagacc tgttctgata
     3181 atacaaaatg atgtaggtaa taaatatagt cctacagtta tagtatcagc tataacatct
     3241 cagattaata aagccaaatt acctactcat gtagagataa gttccaatga atatgggctt
     3301 aataaagact cagtaattct tttagagcaa attagaacta tcgataaaaa gagactgagg
     3361 gaaaaaattg gttgtctaga tgaaaatatg atgataaaag tagatgatgg acttcaaata
     3421 agtttaggat tgtttacttt ttaaagaggg atttgtcttt tattagacaa tccctttttt
     3481 cttttaaata tctcttaagg tttaatttta atgtactatg ttaataaaat taaaaataac
     3541 gcaaaaaaat agcataaaag tgatataata atatggaatt gatataaaat tacgaactca
     3601 agggaggtaa ttgcatgaga gaccataaag gattaaatga gattgcgcgt atcataagaa
     3661 gagatatagt ttcaatgata catagagcca aatctggaca tccaggaggg tcattatcag
     3721 tagttgaaat acttactgcg ttatactttg atgaaatgaa tgtggattct tcaaatccta
     3781 aaatggagga tagagataga tttgtattat caaaaggaca tgctgcacca gcattatatg
     3841 ctacattggc tgaaaaaggt tattttgata aggaagaact gaatggatta aggaagatag
     3901 gaaggatgtt acaaggtcat ccagacatga aaggcacgcc aggtgtggaa atatctactg
     3961 gttctttagg tcaaggtttt tcagttgcat gtggtatggc tatggcatca aagttggata
     4021 atgctccgtg gaatgtgtat actttgcttg gtgatggtga ggttcaagaa ggaattgttt
     4081 gggaagctgc tatgagtgct gcacattata aacttgataa tttaattgct tttttagata
     4141 ataatggact tcaaatagat ggagatatag aaagcgtaat gagtttaggt tcaatagttg
     4201 ataaatttaa agcttttggt tggaatgtaa ttgagataga tggacatgat tttgaccaaa
     4261 tatttgcggc tcttgatata gctaaatcaa cagttaaaaa acctacaatg atagttgcaa
     4321 aaactataaa aggcaaagga atatctttta tggaaaatca agcaggatgg catgggacag
     4381 ctccaagtga tgaggagcta gaaaaagcct tgcttgaatt aggaggtgct gacaatgagt
     4441 aaaatggcga cgagagaagc atatggtaaa gcattagtaa aattaggtca aataaatgac
     4501 aatgttgtag tattagatgc agatttatca aagtctacaa aaacacatga tttttacaag
     4561 tcatttccag atagattttt taatatgggt atagctgaac aaaatttaat aggcgctgct
     4621 tgtggattat caactgctgg taaaatacct tttgcaagta cttttgctat gtttgctacg
     4681 ggaagggctt ttgagattat aagaaattca gtttgttatc caaagctaaa tgtaaaaata
     4741 tgtgctactc atgcaggtct tactgttgga gaggatggag catctcatga aagtgtggaa
     4801 gatattgcta taatgagagc tataccaaat atgacagttc ttgttccagc tgatggagtt
     4861 gaaacagaaa aaattatttt tgaaatagct aagtacaatg gacctgttta tgttagatta
     4921 gggagaagtt cagtaccagt tttatttgat gaagattata aatttgaaat aggaaaaggt
     4981 acagtcttaa gagaaggaaa ggatgtatca attatagctt gtggaattat ggtaaatgaa
     5041 gctctattag cacaagaaaa gttgcaagaa gaaggtataa gtgctagagt tataaacatg
     5101 tcctcgataa aacctataga caaagattta atattagaat cagcaaaaga aacgaatgct
     5161 attgtaactg tagaagaaca tagcataatt gggggattag gttctgctgt aagtgaggta
     5221 gtaggagagt cttgtccaac cattgtaaag aaagttggaa ttaaagatac atttggagaa
     5281 tctggtactc caaatgaatt gttaaaaaaa tatgaattaa cttgtgatga cataataaag
     5341 actgttaaag aagttattat tgcaaaaaga atgtaagaga tataaattat tcatgtttgt
     5401 taataatata atatatttaa aagagaggta taaagtgtta tattaattt
//
LOCUS       DAEOSP010000214         5419 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.218, whole genome shotgun sequence.
ACCESSION   DAEOSP010000214 DAEOSP010000000
VERSION     DAEOSP010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.218"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(438..638)
                     /locus_tag="KRH54_003168"
     CDS             complement(438..638)
                     /locus_tag="KRH54_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG8580800.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(863..1039)
                     /locus_tag="KRH54_003169"
     CDS             complement(863..1039)
                     /locus_tag="KRH54_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580801.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTHLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1093..1182)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1362..1562)
                     /locus_tag="KRH54_003170"
     CDS             complement(1362..1562)
                     /locus_tag="KRH54_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8580802.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(1564..1914)
                     /locus_tag="KRH54_003171"
     CDS             complement(1564..1914)
                     /locus_tag="KRH54_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBG8580803.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            2076..2264
                     /locus_tag="KRH54_003172"
     CDS             2076..2264
                     /locus_tag="KRH54_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG8580804.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGESELKIKKFSILYGSS"
     gene            complement(2741..2911)
                     /locus_tag="KRH54_003173"
     CDS             complement(2741..2911)
                     /locus_tag="KRH54_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580805.1"
                     /translation="MHVYQPKTSEYLLNTSAKSYLSSIVFLFDYITKNESRYAFFIID
                     FPFLSLDIFYKF"
     gene            complement(3300..3788)
                     /locus_tag="KRH54_003174"
     CDS             complement(3300..3788)
                     /locus_tag="KRH54_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBG8580806.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTEGNIIWWTKKDGKITVNSGIGDEDPLAHKIENLGMPYN
                     GSYTLVSSNGSEEYLELNIIIDGEISKSGLDKLEEDYGSSIKIPNDKNMIITFLSNKS
                     DN"
     gene            4594..4968
                     /locus_tag="KRH54_003175"
     CDS             4594..4968
                     /locus_tag="KRH54_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8580807.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINSQSHYKIIDSKEYYTFKKKVLKSSKSIKIMRSLTT
                     THKNVSKEKLDLLDEYYRNLKE"
     gene            5073..5419
                     /locus_tag="KRH54_003176"
                     /pseudo
     CDS             5073..5419
                     /locus_tag="KRH54_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         2183 a          656 c          617 g         1963 t
ORIGIN      
        1 aaaaattaaa aacttaaatc aaattaactt attgtagatt ttttactttt taactaattt
       61 tatatttatt atatttcagt gaaaaaatag aacattgaaa catatagata tgttttaatt
      121 gttttttatt aacaaattac taaaagtgtt atatcagaat tgaatacaaa agaaagagta
      181 tgtatttatg agatagttga aaattttgat gtgccacaac taaaattatt atattactat
      241 atattctaat taaaagagta cttgtaggtt taagtacata ctttataaat atatttttgt
      301 cagatttatt ttataaaaag tatttagact aagtattatt tttaagtatt ttcttgttga
      361 agttattttt tcctttttta caaaatgcgt aaataaaaaa aagactctta aaagagtctt
      421 tccgaaaaat ctatatatta taatatagtt atattttctg cttgaggacc tctagcgcct
      481 ttaactatat caaagcttac tttttgacct tcttctaatg atttaaatcc tgaagtttgt
      541 atagctgaga aatgagcaaa tacgtcatct ccactttcca ttgatataaa tccaaatcct
      601 ttttcattat taaaccattt tactattcca ttattcatta aaaatactcc tattaaccta
      661 tatcaagata tacttgattt acttgtgtat taccacaaat actattgtat catagattaa
      721 cataaatgtc tatttaatta taatttttag ttaacatttt tatttattct taatatgaaa
      781 ataaatttaa acattttata ttaccaatat attaatgtaa atgtagataa aactaaaaag
      841 ggttttctag aaaaaaatct gtctaattat acttacttac caataccatc acttgatgca
      901 agcaagattc cacaactttt tctccgtact ctaatataaa atcatctaaa tgtgtatcta
      961 taaaatcaca ataatcatat gaactaccaa tataagcagt taacatatgt gtacatttat
     1021 ccattttatc tctaaacata atgtatttat ccccctaaaa aactttcatg aaaaacaaaa
     1081 agaaaatatc tttttggcaa ctggctgaca taacttattt tataagcgtt agtccctata
     1141 gctttgcgtc actaaatttc tctagttttg ccgatttagt tttattctac agattaataa
     1201 tacaatagaa ttagttatta ttcaacatat ctagctgaaa aattaataaa tttatatatg
     1261 aacgttaaat tatctgaaaa aataaaaagc ccctctgtca ggagtgactt aaaaattgaa
     1321 tttaagtttg tatttaaaac acatttttaa taatgcaaaa actaatcaaa aataaacaat
     1381 tcatcaatag gtgtatctac tatttttgat atatctattg ctaatttcaa agaaggatta
     1441 tactttcctg cttctaaacg cataatggtt tctcttctaa ctcctacttt ttctgctaat
     1501 tgttcttgag ttaattcttt taattgtcta tattttttta ggttacaagt gaatttaggc
     1561 atattatttt tcctctaaaa tataaagttt tatagcataa acatttaaag tgattgcata
     1621 tgtaattgat agtaaaacac acattaattc tatattccta gacactatag taatacacca
     1681 taaaatgaat aattctataa tagcaagctg tcctgtaaat gccatagctg ctttttcatc
     1741 ttgtacatac ctttcatctg ctttatcacc atttattttt gctataataa agtttgagaa
     1801 aaaagcaaaa aatccaatat atgtaaggtc agcaacattg ccagatgaaa aatatctaag
     1861 acttaaaaat ccaaggaaac cacaaaaacc cataaaaaat tttttctgaa tcatttttta
     1921 tcctccttag taattttaaa agagatatat ttatatcttt atgatacaaa tatatctctt
     1981 ttatattctt ttgtcaatag aaaatcagta ttatacaata tttgtactgc accataattg
     2041 ttagagcaaa aatctactgc ttgagatgtt tatgtatgac taaatatgat tttaatttca
     2101 aattaaaagt agctaaattt tatttgaata gagaaggaag agatatttta atagtaaaac
     2161 aatatggagt ttatgaccat tctcaagttg aaagatgggt taatatatta ggagagagtg
     2221 aactaaaaat aaaaaaattt tctatactat atggttcaag ttagatgtta taaactatat
     2281 gatgataact aaatatagtg tacaataaat cgctaagtat tttggaataa acaatagcac
     2341 agttatagca acatgaaaat aaaaattttt acatagtggt gttgatgcct tttccaaaat
     2401 gaaaggaaga ccataatgaa taaaaacaaa aacactaaag aaaaaataaa ttaacaagag
     2461 aacaagaatt agaatatgaa aataaatttt taaaagatga gcttgagttt ttaaaaaaga
     2521 tgtgtgcctc agggaagcca cattaaaaaa cagtggtatc attattaatc aaaagaaagt
     2581 tagatgtatt atcaaaaaat tagaacttaa atgcatagca ttttttcata aatcacataa
     2641 atataactcc tataaatgta taattggaaa agtaaaaaat aataaaacaa atagaagatt
     2701 taaaacatca atagcatatc agaaaataac aaatgatatg ctaaaattta taaaatatat
     2761 ctaatgataa aaatggaaaa tcaattataa aaaaggcata tctcgattca ttttttgtta
     2821 tatagtcaaa taagaatact atagatgaaa gatacgattt tgcagatgta ttaagtaagt
     2881 attctgatgt ttttggttga tacacatgca ataaagaagt tgtgtttgca gataaattag
     2941 caaaactata gtataagttg caacaataaa gaaaatgaaa attatcatac taaagcataa
     3001 ggcatgttac aattttatta gataggacat taacatacac taaagtactt tttactataa
     3061 agagtactta tttttttgaa attcattaat gtataaacta tcaagaatat tactcaacat
     3121 aatttaaaaa caacttgaat aggtattttt aaattatgtg atataataaa aaatagaagt
     3181 gtatgccaat acacttctgt agttataact aagcattcta ttcttgagga gtgctttttt
     3241 catttttata accaactctt tttactttaa caataattat cagacctata tatttatttt
     3301 taattgtctg atttattaga taaaaaagtt attatcatat ttttatcatt tggtattttt
     3361 attgaactac cataatcttc ttctaattta tctagaccac ttttacttat ttctccatct
     3421 ataattatgt ttaattctaa gtattcttca ctaccattag atgacactaa agtgtacgaa
     3481 ccattataag gcatgcctaa attttctatt ttatgtgcca aagggtcttc atcaccaata
     3541 ccagagttta ctgttatttt tccatctttt ttagtccacc atattatgtt tccttcagta
     3601 tattttgatt catctgcata gtagctaaga gacgcacttt ttattgcact tatatcagct
     3661 tcaagtttag ctatctttgc tttttctata tttttaaata atgctggaag tgctactatt
     3721 gccaatattc ctattatagc aattactact aataattcca ctagagtaaa tccttttttg
     3781 tttttcattt atttcccctc catataaaat taataatata taaattttta ttttgttgat
     3841 ttctactttc tatattttac catatataga caaaaaaatc actttctatg acaaatatac
     3901 tattatttta caatttatgg aaatgattta aaatgacatt tcttgttaga aaagatataa
     3961 gcaatggatt aaaataaaga aaactacagt catatcaacg ccaataatat tatttaaaag
     4021 cagatgaaaa taaaaagagg gcccagaaaa taatgtctat ttaattatac ttacttacca
     4081 ataccataac ttgatataaa caagattata ctacattttt ttatgttcta gtataaaatc
     4141 atctaaccgt gtatctataa aatcaaaata attatatgaa ctatcagtat aataagtcaa
     4201 aatatgtata catgaataca tattttttaa aagatatata ttattacata cctaaaaaac
     4261 tttcataaaa ataaaaataa aatatgattt tagtaacttg ttaacataac ttataattta
     4321 ttttataagc attagcctat atagctttgt gctactaaat ttctttgatt ctaacgattt
     4381 agtgctattc tacaactaaa ataatacaat agagctattt attgtttggt agcattgttt
     4441 gcaaattaaa caaatttata tatatgaata ttattttacg ttcaaaaaaa cagactaaat
     4501 atgtagaata ttcggtaaaa cttacctcta gtttttgtct taaaaagctc ctattattaa
     4561 cttaagttag aagaattaag gaagtgacat aaattgaaag aagaaatatt aatagaaaaa
     4621 ttattaagag cagaattaat agtaatggag tatttatgga aaaaagacac tctaatgcct
     4681 aaaaaagaaa ttgtaaaaga aataaaacag atttatggat ggcataaaag tacaataaaa
     4741 atactactaa aaaggctagt tgctaaaagg tatttggcta gatatattat aaattctcaa
     4801 tctcattata aaataataga tagtaaagaa tattatactt ttaagaaaaa ggtactaaaa
     4861 tctagtaaaa gcataaaaat aatgcgttca cttaccacta cacataaaaa tgtaagtaaa
     4921 gagaagttag acctattaga tgagtattat agaaacttaa aagaatgata tagtaaaaat
     4981 cgaatgtaat tatttacctt aattcttttg tttgtaaaac tttattgata cataatttaa
     5041 catatacata aattggtaaa ggaatgtata ctatgaaaat tagtaaatta ccagaagcgg
     5101 aattaaaagt tatgagatat atttgggaat cagaaaaggt attaatatca agagaaatca
     5161 ccaagtctat ggaacaaaaa tatgaatgga aagatacaac tatatttaca gtattaaaaa
     5221 gattactaaa gaagagagtt tttagaaacg gagaaaatag ataagtgtac acactataat
     5281 gtattagtaa aaaaaaataa tatcagagat tttaaacaaa agaattttta aagaatacac
     5341 ataaaaattc aatattaagt ttaatgtcag cactatgtag taaggatgat gaagtagatg
     5401 gtgatatttt aaatttatt
//
LOCUS       DAEOSP010000215         5404 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.219, whole genome shotgun sequence.
ACCESSION   DAEOSP010000215 DAEOSP010000000
VERSION     DAEOSP010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.219"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(524..1930)
                     /locus_tag="KRH54_003177"
     CDS             complement(524..1930)
                     /locus_tag="KRH54_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8580808.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRNGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQTLNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKGQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(1975..2682)
                     /locus_tag="KRH54_003178"
     CDS             complement(1975..2682)
                     /locus_tag="KRH54_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8580809.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            2853..3767
                     /locus_tag="KRH54_003179"
     CDS             2853..3767
                     /locus_tag="KRH54_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580810.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPEKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3767..4528
                     /locus_tag="KRH54_003180"
     CDS             3767..4528
                     /locus_tag="KRH54_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8580811.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIINSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4540..5277
                     /locus_tag="KRH54_003181"
     CDS             4540..5277
                     /locus_tag="KRH54_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8580812.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         1867 a          834 c          704 g         1999 t
ORIGIN      
        1 aaatactaca caaaaactct actttagtag aacttaatgg cgcaaaatac ttcaatttaa
       61 aacatttatc gtactttaaa taaataacta taataatata cttacaatag ttacatagaa
      121 gctattataa ctacacagaa cagcgtataa ttaaaacttt tttaggatgt cattatattt
      181 tttcttaaaa ttaaaaaaca ctataccata attctatttt agtagaattt ttttgtcaat
      241 aataaaatta aatattaact aaaaaataaa aaaaatggaa ttatctaaag aatttaataa
      301 ccttacaata attccaaaat attatttcta tgaaaatttt gataccatga gtaactcagc
      361 cataagcgca taaagcacaa gttactcttt actttaataa aacttgacaa gaaaagaatt
      421 tatccttatt ctactctaat agagttaatt tgtcaataaa gaataaaatt attttcaaaa
      481 tattttcatt cttcattctt atctgttatt attttataaa atatcactta attagaggta
      541 attttagtat tgtttttagt ccctgactat caatgctcgt aatatctaat ttacctctat
      601 gtgcattaat aatgccagat actatcaaaa tacctagacc atgattttga cctttggcat
      661 atgcctcact tagtccattt tggcccttta ataaactatt aattctttct tgactcatac
      721 cttttccatc atcttcaaca attatatagc aaaaatctaa atcagactta gaggtaaccc
      781 ttatattaca tccttgttgg ttatgagtta tactattttg tattaaatta ctaatagccc
      841 tttttaataa ttttttatct gcatctataa taatatcttc atttagtata tcaagttcaa
      901 cgtaaaagtc attatccaaa ttattattta taaactcaga aataagctct ctcaaaaacg
      961 cagaaactct aatcttatca caattcaatg tttgcatatt atattcaagt ttagacacta
     1021 aattgaggtc atttactaat tctctgattt taatcccttg attgcatata atagacactt
     1081 gctcttgttc tctatttgaa aggctactgc tttcttcaag ttctccagca tatccaatta
     1141 tcattgaaag tggagttctt atatcatgag atattcctgc aatccagtta gacctagctt
     1201 cctctttctt cctaagtttt tcatctttca tttgcaactc tttagataca gtattaatag
     1261 attttgaaat atcacttagt attccttttt cttgtaaaag tactggttct ccttttgaca
     1321 taagctttat gccagtaata attggtttta tagatttcac catttttaaa cctatgaata
     1381 gagctattaa aaaagtaact attgcattaa ccagaaacat acctattatt aacataggaa
     1441 attgttctat ttccttaacc gtataagtaa ggttaaactt agtgtacttg tcttttggat
     1501 aacctgttac taatattcca tttggatgtt cccatacata cacaggatag tcctttaaaa
     1561 agaaccttga aaactttgct acatctgtca aagtataatg acttggaatt tcttttggta
     1621 aatcaaatcc ccatattcta tctccattgt tatcaattaa cattgcccaa actttatttt
     1681 tatgtagtac ttccttacta gaatcatcta aaataaactt tccatttctt aaaatcaaat
     1741 tatcttcaat cataatcaag tttgatttta tttctctgct atcacttgta tctaaattat
     1801 cagagtattt agatacaaat agcatatatg aaaacagaag aaaacctgct atatttaata
     1861 caataaaaag taatgaaatc aagatacttg aacctataaa tcgcctcaat atgtacttta
     1921 cactatccaa aattaaatca ctcctcttga tttcttttat tctaatatct aaaattaatt
     1981 aatcatcaac ttatatccaa gacctcttac tgtaataaga tgtttaggtg tagatggagt
     2041 tttttctatc ttctctctaa ctcttctaac atgaaccata agggtatttt catatccata
     2101 catatcatct ccccaaatgg cttgacatag tgaatcatta gtaacaattc tccctttatt
     2161 ttcatacaac tttaaaagca acgaatgctc tttagctgtt aaagaaatct cttgcccatc
     2221 tttctcaaca catgcactat ttaaattcac aacaacagaa tctcccagtt taaatactgg
     2281 caactcctct tcttctatag gcacatatac acgatttaat atacccacaa gccttaaagt
     2341 aagttccttt ggtaaaaatg gctttactat ataatcgtca gcccctaagc ccaaaccaat
     2401 caatctatct tcatcttcac ctcttgcaga gagaaaaatc actggtattt tagatgtctc
     2461 tctaattttt ctcataatag aaaaaccatc tccatctggc aatacaacat ctaaaattgc
     2521 acaatctggc tttacatttc taaataccct aacagcttcc tcataacttg atgctaaaaa
     2581 tactctacta aacccctctt ttctcaggat ttcatctatc atttttaata attccttgtg
     2641 gtcatcaacc acaagtattt ttttatcttt tattgactcc ataatcatca ccctccttta
     2701 gtataccata tacccatttt cacaactttg cctaaataga cttttaaggt tattgtaagg
     2761 taaatttcag ctaattgaaa tgtaaccaag ttattatact gatataagtt aaaagaatca
     2821 ataagcacag attaaaaaag gggatgaaac atatggaaat gataattaga acacaaagac
     2881 tttcaaagga atacaataaa atatttagag taaaggatat agacctaaga gttccacaag
     2941 gagctgtata tggttttttg ggtcctaatg gagctggaaa atctaccact cttaaaatgc
     3001 ttcttggact tgctaaaccc acacaaggaa atattaatat tttaggtaaa gagttaaatg
     3061 aaaaaaatag aatatcaatt ttaaaagata taggttcttt gatagaatct ccatcatact
     3121 atggtcatct tacagggctt gaaaacatga ccataatgca aagactctta gatttacctg
     3181 aaaaaaatgt aaatgaagca ttaaaaattg taaggttaga aaatcaaaaa aataaaaaag
     3241 ttagtcaata ttctttaggt atgaagcaac gtcttggcat tgctatggca ataatgaagt
     3301 ttcccaaatt attaatactt gatgaaccta caaatggact cgacccagca gggattgagg
     3361 aaattagaga attaataaaa tctctaccag aaaaatacgg gatgactgtt ctaatctcta
     3421 gccatctttt aagtgaaatt gaccaaattg ccacatcaat aggaataata aatcatggcg
     3481 aactggtttt tcaagatagc ataaaagaac ttcataatag aggtcaaagt cagattgcta
     3541 tcaaaactaa gaatgttgta tctgcacaaa aactattaaa tactaaagga tatatgtcac
     3601 ttatacaaga tgggtacttg actattgaaa atttaaaaga ctcagatgtg gctaaaataa
     3661 atggtatgct tgtccattca aatatagatg tatttagaat agaagaacat aaaaaaagct
     3721 tggaagatat attcttagaa cttactggta aggcggtgac tttataatga aataccttgg
     3781 tctagaattt tataaactta aaagaaagaa gattattctg atgacctttt tatttgtagc
     3841 tgttgaaata atgtggtgta tcataaattc aaatagagct attacacgta atccagatat
     3901 gttaggtgga tttgaatatt cctatatgct tatgagtttt gcaagtttaa atggtctatt
     3961 ctttcctatc ttaatatcaa taattacatc acgtataagc gatatagaac ataaaggaga
     4021 tacatggaag cttttaaaat cttctgtgac atctttaaat agtatttatt tatctaaatt
     4081 tttatgttct gctattttgg tatctatacc tgtattgatg caagtgctat ctattgtgtt
     4141 gtttggacat tttagagggg ttattgagtc aatttctgta tctttacttt taaaatacac
     4201 attaggaaca attcttgtaa gtattgctat cattgcacta cacctatgga ttgcaactgt
     4261 aattccaaat caaatgattg caattacatt tggcatgatt ggtagtttta taggtttgac
     4321 atcaggtttg ttttatcaag gtattcgtag attgtttata tggtcttatt acctagagtt
     4381 atcaccttta agttatgctt atgataatgt tttaggttca tctgtctata gaattgatat
     4441 gaatttttct attacgattt tagttttctt aattggaatg ctaatttatt atattggaag
     4501 aaatcattta ttaaaaaaag aagtatagga gggatttaaa tgttaaggaa gtcagttttg
     4561 ggagaaattc taaagttaaa aaattctttt atatggtatg tattattagc tttgccactt
     4621 ataagtgtgt tgattggttc tggtaatttt tatctaaatc aaggcatact caaaaaagaa
     4681 tggtacagct tatggactca agttagtctt ttttatgggg aatttttctt tcctatctta
     4741 atcgcaatct tttgtgcata tatttgtagg cttgaacata tgaatcataa ttggaataat
     4801 gtaatgacac tgccagttaa tttaaaaaat atctttttat ctaaacttac agtagtttca
     4861 atacttacag ctattacaca agttttcttt gttatctttt atatagttgc aggaaatatg
     4921 tttggttttt ctggtagtgt acctaaagaa ttgactggtt ggattgctaa aggttggatt
     4981 atttctattt caatagcttc tatacaatta tacatgtcta taaagattag aagttttgca
     5041 actcctattg gtattagtct ttgcctttct ctatttggta tgggattaat agtagcaaaa
     5101 cctgctgttg gtatattttt cccttattct atgttaggta gtgctatggg ggttataaag
     5161 caatctgctt taaatgttaa tgacactata gttacaatat gtgttgcatt tattactact
     5221 atagtatttt ctgaaattgc caatagaaat tttagaaaga atgatgttat ttcttaatgg
     5281 taaatgctag atattttggg gtttgaaatt tatattcttt ttagttgaaa tctatatcca
     5341 ttttatttaa aaatatatat cttttatttg aattttatat gttttatcta ctcgctatat
     5401 aata
//
LOCUS       DAEOSP010000216         5400 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.220, whole genome shotgun sequence.
ACCESSION   DAEOSP010000216 DAEOSP010000000
VERSION     DAEOSP010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.220"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(34..1437)
                     /locus_tag="KRH54_003182"
     CDS             complement(34..1437)
                     /locus_tag="KRH54_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8580813.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQLFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(1421..1945)
                     /locus_tag="KRH54_003183"
     CDS             complement(1421..1945)
                     /locus_tag="KRH54_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8580814.1"
                     /translation="MHEQIDCCERKKRTIDIKGENMRELLKWVSIINRYNTNYIDRKL
                     ADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGVK
                     DKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMN
                     EEAKLEAKENGRKI"
     gene            2225..2740
                     /locus_tag="KRH54_003184"
     CDS             2225..2740
                     /locus_tag="KRH54_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG8580815.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGNKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            2789..3292
                     /locus_tag="KRH54_003185"
     CDS             2789..3292
                     /locus_tag="KRH54_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBG8580816.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            3289..3939
                     /locus_tag="KRH54_003186"
     CDS             3289..3939
                     /locus_tag="KRH54_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580817.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDNTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(4074..4853)
                     /locus_tag="KRH54_003187"
     CDS             complement(4074..4853)
                     /locus_tag="KRH54_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG8580818.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(4965..5216)
                     /locus_tag="KRH54_003188"
     CDS             complement(4965..5216)
                     /locus_tag="KRH54_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBG8580819.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
BASE COUNT         1943 a          867 c          643 g         1947 t
ORIGIN      
        1 taaattaatt ttctatactt cttaaatatt gtcttatacc tctgcaatct tattatggtt
       61 actcatcaaa ttattttgct ttatagtcaa tgatttcatt tcatgaactg caaagtatat
      121 acataccatt gccgctgctg catctgctat tggtcctgca aacattactc catcaattcc
      181 cataaacatt ggtaaaatta ctatcaatgg aattaaaaat aaaatttgac gagtcaaaga
      241 agtaaaaagt cccaacactg atttcccaat tgatgtaaag aaagttgatg atactatttg
      301 aacaccattt ataaatgtta agaacaagaa agttctaaaa taatgttctg caaatataaa
      361 ataagtctca tttccgttac caaatataga cgtaatttgt cttgggaata attggaaaca
      421 taagaatgca ataacagata taatagttgc acttcccact gcaaacttat atgcttcttt
      481 tacacgatta tacttttcag ccccataatt ataccctata attggttgca taccttgtga
      541 tataccaata attatagata taaaaatcat atttacttta ataatgatac cgacacaagc
      601 aagtggaatc tcacttccat aaactgacct agccccataa tatgccaatg tattattcat
      661 aactatctgt gttacagtca ttgctaattg attaaaacat gagcttgaac ctagcgcaaa
      721 tatatgttta taaattatac tatctaattt aaaacttttc ttatgtaatt taatatgttg
      781 aaaattcttt atataatgaa cacttattcc aaaagatata agttgcccaa taacagttgc
      841 taaagcagac ccagcaattc ccatattaaa aacaaaaatg aataatggat tcaaaattgt
      901 attaataact gctcctatta acatacaaaa catagaagct ttcggtctcc catctgcaag
      961 tatcaactta ctcattccag tggacattat aagaaatggt aatccaattg atgtaatttt
     1021 tgtatatgtg tgtgcataag gtagtatttc tgatgtagag ccaaagaatt ttaatagtgg
     1081 tgttaaaaat attgatacta ctataaataa acttattcca aaaacagcca tcaagcatat
     1141 agaatttcct gctgcttttg tagctttttc tgaattttta gcacctaatt ccaatgaaca
     1201 cttagacgct cctccaattc ctaacaataa tgcaattgca gtacatattg ttgttagtgg
     1261 aaaagtgaca tttgtagcag cattgcccaa tacaccaaca ccttgcccaa taaatatttg
     1321 gtcaacaatg ttatataaag cacttactaa cattgctata atactaggaa ttgcgtactt
     1381 agttaataac tttgaaacct tttcatattc caatggctcc ttatattttt cttccatttt
     1441 ccttcgcctc caattttgct tcttcattca tatcaatagc caaaaatgca actttcttta
     1501 acattgacat aaacaactct tgctcttctt tctcaaatga actcaacaac tcctcaacat
     1561 acccatttgt tatttttagt atttcatcat aacatcctaa agatttttct gtaggatata
     1621 aatgccatgt tcttttatcc tttacacctg gttctcttgt tataaagcct tctttttcta
     1681 aatttgccaa agctcttgtt atattactag gattcacaag aattttctca aatatagtgt
     1741 cctgtgtaat acctggatta ttacatatgt gtaatacata aaaatattgg ctattattga
     1801 ttccaatatc cgccaatttc ctgtcaatat aatttgtatt gtatctattt ataatggaaa
     1861 cccattttaa tagctctctc atattctctc ctttaatatc tattgtcctt tttttgcgtt
     1921 cgcaacaatc tatttgctca tgcaattatt ctactgctaa ttttatactt tgtcaatagc
     1981 acacaagttt atatttaaac cataaagtta ctataaattt tatatattag actcttactt
     2041 tgatttaact atttttaaat ctttattgat atttaattac cttttatatt aatttttttg
     2101 catatttatc aataactcct aattcttttt agcttatctt aaaaagcatc ttgacgtaag
     2161 atttattatc tgtaataata aatatatcga atcgatatat atctaattga taccaggagg
     2221 taatatgtta gaatacatta ttttaggatt tttaatggaa aaagaattaa gtggatacga
     2281 cttaaaacaa ataatgtcag aaagtacctc gtactttttt gacgccagct ttggaagtat
     2341 ctatcctgca cttaaaagat tagaaacaaa aggatatata cactatcacg aagttataga
     2401 tggtaacaaa ttaaaaaaac tatattccat aactaatact ggtaaagaag tatttcttga
     2461 atggttaaag aaacctataa atttttctaa aacaaagcaa gaccaccttg ttaatatatt
     2521 cttttacaag tatctaccca aagaaataat tgaaacaaac ttaaaaatgt taataactga
     2581 agtagaatta cttctaaata aattaagtaa acaaaaaaat gatgcagaag aaaatcattg
     2641 tgttgaccaa tatacatata tgtatgcaac aatgatttat ggagttgact attataattt
     2701 tgttattaat tggtgtaaag atttactgaa aaatctataa gatataattt ttagaagtaa
     2761 acatacataa taaaaggaga tggactttat gaggctttta atacatgatt taactgacaa
     2821 agaattagaa aagttattac ccaaacttga tgacaatata aaaatacttc caaacacaaa
     2881 agaaatccac aaatgtattg gttgttttgg ttgctggatt aaaactcctt ctgaatgtgt
     2941 tgtaaaagat tattacagca acatgggtaa acttataaat gactctgatg gaattattat
     3001 aataagcaga tgctgttacg gagggtttag cccttttgtc aaaaacgtac ttgataaaag
     3061 cattaattat attcttccat actttgtaat aaaagataat atgatgcgtc ataaaagcag
     3121 atatgataaa cagataaatt taaaagctat attctatgga gattgtatta cagaagctga
     3181 aaaagaaact gctacgaaat tagtaaatgg aaatgctata aatttcaata taaaagaaca
     3241 ctctgtttgt ttctatgaaa atatacaaga tataagaggt gaatccatat gaatatagta
     3301 atgataaatg gtagtccaaa agtaaataaa aataattcac agtatttttt atctgaatta
     3361 aaacaactta ttcaaaatga taataaagtt tttacattta aagtagataa cacatctaag
     3421 tatcctgacg ctcttaatgc catacttaat tgtgatagat taataatcgc atctcctctt
     3481 tatgtagatg gtattccatc tcatttactt cattttttag agaaatgtga aaaattttta
     3541 aaaacacaaa ataaaaaacc tattccagtg tatgcaatag ttaactgtgg attcaatgaa
     3601 ggtgaacata atcatttggc aattcaaatg atagagcatt ggtgtaaaaa aatcaacttt
     3661 acatgggttc aaggtattgg aattggagct ggggagatgt ttggttcact agaatccgta
     3721 cctttgggta aaggacctaa aaaagattta ggaattgcat taaaagaact ttcaaaaaat
     3781 atacttagtg ataatataca tatagatgat tccaacaaaa gtactatttt tatttcacct
     3841 aacttcccta gattcctata taaacttatg gggaatcatc aatggaatac tcaagctaaa
     3901 tcaaatggat tgactaaaag tgatatacta aagaaataaa attttacttc aacaaatact
     3961 aaaaaccgtc aagcacgaat aaactattca tcttgacggt tttttatgtt atttatattc
     4021 actagacatg ggataattca ctataacatt gattatctag tctaatattt atcttattct
     4081 ttttgtaaaa tccaagttgc tccaacaagg tcctgctgta aatcagtata ttcatttttt
     4141 tggctaataa catattgttc aaacatatct ttttgagaag gatatttttt aattagctca
     4201 tttgcacgag ttataatcca attcatttcc tcttcaacat cattttcaag ttctaaacat
     4261 ggtatacatt caattagctt cagacctaat tctttgaaca atatacacca ctcattgtaa
     4321 gtgggataca ccctctcaaa attaagctgt tcatttgtag atttatctac tacatatgca
     4381 tcatcaatta aaatgaaccc cttacttttt attgtaggta atagcttact tatagtttct
     4441 ttataattac ctaaaatatc ccctacagct ccaaaaatca cacaatcata atcatgctca
     4501 atttttacag aactattagc atcattcatt tcaaaatcac acaaagcatc aaccttatat
     4561 tcaatagcct ttcttctagc ataatcaata aattcaggta tcaaatcaat acctttgata
     4621 tgtatattca actcttttgc aagcttcaca gatacagcac cttttccaca agctaaatcc
     4681 aaaacttttg tatcctcatt aaaaggtata tgctttctaa ataattgtaa catttgttct
     4741 ggtgaacttc ctaattccca aaaatcttgt aataaataag gcaaataagg taaaatatca
     4801 gtagtatcag aagttagtga ctctgccaat ttttctttta cagtatcatt cattttttca
     4861 acctccaaaa tttaaattat tattcaaaag taatattatt ttatatactt cttcataggt
     4921 actacaaaca gtggaaaatg cttttgacct ttgtattttg caattcattt cttttcttta
     4981 tgagctgtaa taattgtata actgtgagcg tttactgtta taatacaatc atatacactt
     5041 atataccagt tttttccctt tctattaata atggcatttg gagattctat tttagtttta
     5101 caccagttca ctacatcatt tgtctccaaa gaaagatttt tcttaattct tacaacgcct
     5161 aactctgttg tatgcaatct atctaggttt ttaagtaatt cgttttctac attcattttt
     5221 attaaaacac tttatccttt gcaaaattaa atctattatt tagtactatt ttatcatagt
     5281 attgtgtatt ttatcataat cttatagtaa aactatttct ttcctacaga ttataaatta
     5341 tttatgaata cacaccatta tagaactttt cctatgcaaa attaaatcta ttatttaaca
//
LOCUS       DAEOSP010000217         5394 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.221, whole genome shotgun sequence.
ACCESSION   DAEOSP010000217 DAEOSP010000000
VERSION     DAEOSP010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.221"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     misc_binding    698..929
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1037..2518
                     /locus_tag="KRH54_003189"
     CDS             1037..2518
                     /locus_tag="KRH54_003189"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBG8580820.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            2528..3421
                     /locus_tag="KRH54_003190"
     CDS             2528..3421
                     /locus_tag="KRH54_003190"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBG8580821.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            3545..4606
                     /locus_tag="KRH54_003191"
     CDS             3545..4606
                     /locus_tag="KRH54_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBG8580822.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(4761..5273)
                     /locus_tag="KRH54_003192"
     CDS             complement(4761..5273)
                     /locus_tag="KRH54_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBG8580823.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
BASE COUNT         2012 a          663 c          798 g         1921 t
ORIGIN      
        1 attaattaac tttattttac atatatataa aaaaactgca aataaaaatt aacttttatt
       61 tacagttctc acttatttat ataattcaaa accttattgg ttatcattta cagtgtcttc
      121 taatatattg ctttctgata tatatgatag aattgaaagt ccatacataa ccaaaactaa
      181 tgctgctgtt ataagtacta ttcccatatt taatacctcc ttttgtttac agtatactat
      241 tattatgtga aatatttatg aatttaattt gaatatatat gtaatttcac actaacaatt
      301 tcttcaaatt aaacattatt aactatatac ttttgttgaa ccaattatga ttaatagttt
      361 aaatattagc actatttact tatatttata tgatatacca tataattaca tttttcaaat
      421 taaagttttg taaacttttt ttgtaatata gtgtagtata aaattgtaat attacagata
      481 tttaatagtt atcttttaat tactttcatt aaaaacagct tcatcactat gaaaatctag
      541 gtttttaaac ttttaaaaaa gttaaaaaaa tatttttcta atactcaatt tgattacaat
      601 ttttttacaa tttaaaaaat attttttcca aatttttata ttaatacttg actttgtctg
      661 ttttataaac tagaataatt aataatatta agaatattga aaggcattga ttaggaagag
      721 taaatctgaa tcgattacca gagaaggaaa ctactagctg aaatgtttcc ctaattagaa
      781 ggattgaaaa ctacctagga gcttctatcc gaagttatct taatgatttt agtaggaata
      841 gacgttttta ttacttgcgt taaagtatca agtggatttt ttaaaatcaa tttgggtgga
      901 accgcgggaa acagattctc gtcccttttt tagggatgag ggtctttttt tattacctaa
      961 attcagtttt taaaatattc tgtatctaat ttaattagct aattaaaaat ttaaaatata
     1021 tggaggatta taaaaaatgt attattcaag tacgagaggc accgaagaaa aagtaactgc
     1081 atcacaagca ataataaaag gaatatcaaa tgatggtggt ctttatgttc caagtgaatt
     1141 tcccaatgta aaaaatgaac ttataaattt agtaaattta acgtattctc agatagcatt
     1201 ttttgtactt agtaaatttt tatgtgattt tactgaagat gaaattaaaa attgtataga
     1261 aaatgcatat gatgaaaagt ttgattgtag tagtatagct ccattaaata aagtaaatga
     1321 tacttatttc ttagaactat atcacggtcc aactttagca tttaaagata tggcacttac
     1381 tataatgcct catcttctta aaacatctat aaagaaagat aatttagaga aagatgttgt
     1441 gattcttact gctacatctg gagatacagg taaagctgca cttgagggat ttaaagatat
     1501 agataaaata aagataattg tattcttccc agaagatggt gtaagcccag tacaaaaact
     1561 tcaaatgaaa actcaaactg gaaaaaatac atatgtagtt ggtataaaag gaaattttga
     1621 tgatgcacaa tcaggtgtaa agaagatttt ttctgataaa gaatttaatc aaaaactatt
     1681 ggataataat tatatattat cttctgcaaa ttcaattaat ataggaagac ttcttccaca
     1741 agttgcatac tatttttatt catatatgaa attagttagc aatagtgata taaatttaaa
     1801 tgataaaata aattttgttg tcccaactgg taattttggt aatattctag ctggatatta
     1861 tgccaaacaa atgggtcttc caataaacaa attaatatgt gcatcaaatg ataataatgt
     1921 tctttatgat ttctttaaaa ctggtgtata tgataaaaat agaacattga aattaactac
     1981 ttctccttct atggatatat taatttcaag taatttagaa cgattactgt ttgaaatatc
     2041 aaatagaaat actgatattg taaataagct tttatcagac cttagtgata aaggagtcta
     2101 caaaataagt gatgatatgg aaaaaaattt agcctcattc tatggagaat attcaaatga
     2161 agaagaagtt aaaagcacga taaacaatgt gtttaaaaat tataattatt tgatagatac
     2221 tcatactgct gttgcataca attgttatga aaaatataaa aaagaaactt ctgataacac
     2281 aaaaactgtt attgtaagta ctgcaagtcc attcaagttt tctgaagatg ttctaaagtc
     2341 tatagattgt gattttaaag accttgatga ctttgctata attgatagat tatcaagtat
     2401 ttctaaaata gatattccaa aacctattaa aaatcttaaa aatgcagaaa tactacataa
     2461 agatatttat gaaaaagatg aattaaaact agctataaaa aaattcttaa aggtataggt
     2521 gattattatg ttagagatta tagttcctgc aacaagtgct aatataggtc caggatttga
     2581 ttgtcttgga attgcgttaa atatatacaa taaattttat gttgaagaaa ttgaaagtgg
     2641 tcttgaaata gaaggttgtg aagatgctta caaaaacgaa aacaacttag tttatacatc
     2701 tatgaagtac ttttttgata gagttaaacc agaaaaaata cctgctggta tcaaaataaa
     2761 aatccaaagt gaagtaccta tatgtagagg tcttggcagt agtgcctctt gtatagtagc
     2821 tggagttata gctgcaaatg ctctatcagg tgctaattta gataaaaatc aattactaaa
     2881 tatagcttca gaaatagaag gtcacccaga taatgttgct cctgctattt taggaaatat
     2941 gattgtatct gttactgata atgagaatat acattatgat attataaaaa ttcctgaaga
     3001 acttaaattt tgtgcaatga tacctaattt taagttatca acagaaaaag ctagaggtgt
     3061 tttaccaaag gaaataccat attctgatgg ggtattcaat gttagtagag ttgctttact
     3121 aatatctgcc ttactaaaca aaaactttga cctacttaaa gtagcttgtc aagacaaatt
     3181 gcatcaagat tatagaggaa ctctaattga gaactacaat gatattgttg aaaaatctga
     3241 acaattaaat agtattggag tatttttaag tggagctgga cctactataa tgtctttaat
     3301 aaaagaaaat gatgatagtt ttgtggataa tatgaaaaat tacctacaaa aactaaaatc
     3361 tgattgggaa ataaaagagt tatgttgcga ttctaatgga gctgttttaa atataatata
     3421 attatcgtct tctatacaaa atataactac tcttttatta taatttgtag tataataatt
     3481 aggactaggt aataatatta agtattataa tctagttctt taaacatgtt tacattgagg
     3541 agggattatc ataaaagagt taataattag agatttagtt gcaaaagttc ctataattca
     3601 aggtggaatg ggcgttggta tatctaggtc atcactagct ggtgcagttg ctaagcttgg
     3661 tggtgttggt gtaatttctg gagttcaaat tggatatgat gaggaagatt ttgaaactaa
     3721 tactataaat gcaaatttaa gagctataaa aaaacatatt tctaaggcca aagaaatctc
     3781 aaatggtggt attattggta ttaattttat ggtagctatg aaagaatatg aaacatacgt
     3841 aaaggaagct gttaaagctg gtgtagacct tataatatct ggagctggat tacctaataa
     3901 gctaccatca ttagtaaagg gaagtaatgt aaaaatagca cccatagttt caactgcaaa
     3961 agctgctaat gtaattttaa aaatgtggga tagaaaagag aaaactactg ctgacttaat
     4021 agttgttgaa ggtccaaaag ctggtggtca ccttggatac tctaacgagg aacttgataa
     4081 catagattct atagactatg ataaggaatt tgttgaaatt ttaaaggttg caaatactta
     4141 tggagaaaaa tttggtagaa acataccagt agttgctgct ggtggtataa cttctagtag
     4201 tgatgttaaa aaatatatag atatgggtgc aagtggtgta caagttggta caagatttgt
     4261 tgctacttac gaatgtgatg cccatgagaa ctttaaaatg gcatacataa atgctagtga
     4321 agaagatgtt catatagtta aaagtcctgt aggtcttcct ggacgcgcca taagaaacaa
     4381 atttatagaa gaagttaaaa taaatcgtcc agaaataaag aagtgctata attgtttgat
     4441 tccttgtaat ccaaaagaaa ctccttactg tatttctcaa gctctaatca atgcagttaa
     4501 aggagatgtt gaaaattcac ttttattttg tggaaatgat gcctataaaa ttgataaatt
     4561 aagtactgtt gaagatgtta tcaatgaatt gatttctgaa ttataattta aagaaaacta
     4621 aattaagatt ttatcaagct aaggagttta aatacgttat ttattaatct ttataaacat
     4681 atttatgtcc ttagcttttt tgtgtcaatt tccttttatt gaaattttaa tttaattaga
     4741 gatagtatta acccttataa ctaatcttct tttacaagta cattggttat ttcagctata
     4801 tataaagtat gataatcttt ttgtggatac catcttccat ctataccttc agcaataaaa
     4861 cattgaggtt caagtttctg actatacatt tttttgcata taatagccat tttagattct
     4921 ttaaaataag gtgtatcatt taagtgttct atattaaatc caatctgtga tatcttatcc
     4981 tcttctctac cagatacctt tccacaatat gaaagctctt tacgaaaatc ttcatcaaaa
     5041 aatgtaagag aaaatttatc tgtattatct ataaattcct tagtataacg ctgtggtctt
     5101 attactacaa aagcaacatc ttttccccac ataactccaa gccctcccca gctagcagtc
     5161 attgtattca ctttaccttc attttctgct gttataagtg tccaatcttt acctatcaat
     5221 ccaaatggat tatttttaat ctcttctgcc aaaatttctt taaaatcact catatctcta
     5281 ataacctctc attctcttat atttatattc cttttatttt cgtgataaat tataaatgaa
     5341 gtctagtttt gaaaataaaa tactttttta aatatattat tgcaaataag taaa
//
LOCUS       DAEOSP010000218         5385 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.222, whole genome shotgun sequence.
ACCESSION   DAEOSP010000218 DAEOSP010000000
VERSION     DAEOSP010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.222"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            153..2753
                     /locus_tag="KRH54_003193"
     CDS             153..2753
                     /locus_tag="KRH54_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBG8580824.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTAPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLGTPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHYNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
     gene            2857..3435
                     /locus_tag="KRH54_003194"
     CDS             2857..3435
                     /locus_tag="KRH54_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBG8580825.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            3470..3946
                     /locus_tag="KRH54_003195"
     CDS             3470..3946
                     /locus_tag="KRH54_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBG8580826.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            4465..5286
                     /locus_tag="KRH54_003196"
     CDS             4465..5286
                     /locus_tag="KRH54_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG8580827.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSMYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
BASE COUNT         2192 a          512 c         1063 g         1618 t
ORIGIN      
        1 tatgttaact caaatgaacc aaatataaat aactaatata taaattgatt gaagaattta
       61 aaatatagta agaaatatat aaatttaata caaatattta agttatagat ataattatat
      121 taacctaaca agtataaata gaggagaaaa atatgaaaaa aatagaatta cttgcaccag
      181 taggctcttt tgattcatta aaagcagcag ttcaaaacgg agcaaatgct gtgtatttag
      241 gtggtaaaga ctttagtgct agagcatctg caaataactt tgatagaaat gagctgattg
      301 aggctgttaa atactctcat ataaggggag tgcaagtctt tgttactgta aatacattga
      361 taaaacagta tgaaatagaa gattttgtag agtatgtaaa atttttatac gatatagata
      421 ttgatgcttt aatagtacag gatataggaa tggcaagact tataaaaaaa ctattacctg
      481 actttgaact acatgcaagc actcaaatgg tagcacattc cttagaagat gtaaaatacc
      541 ttcagagtgt agggtttgat agagttgttt tagcaagaga attaaatgtt gatgagataa
      601 aatggatttg tgaaaataca actgtagata tagaagtttt tgtacatgga gctttatgtg
      661 tatgttactc gggtcaatgc cttatgagta gtatgatagg caataggtcg ggaaatagag
      721 gtaggtgtgc acagccatgt agacaaaagt atgagttgat agatataaat acaggagaag
      781 tagtaaaaag taatggagat tatttgttga gtcctagaga tttaagtact atagaagatt
      841 tggataaaat aattgaagca ggtgtattat ctttaaaaat tgaaggtaga atgaaaaagc
      901 cagagtatgt agctactgtg gtttcaggat atagagaagc gattaatgaa tttgtagata
      961 agaaaaaaat aagtatttct gaggaaataa taaataattt gtataccata tttaatagaa
     1021 agtttacaaa aggatatata ttaggtgaag ttggtaagga tataatgaac tctaatagac
     1081 ctaataatca agggctatat attggaaaag ttttagatta taatagaaag aacaaaagac
     1141 ttagagtaat gctagaaaat acgcttaaga aaggtgatgg aattaactta ggtggaggta
     1201 ctattggaag aataataaag gatggagaga taactacaat agcacataaa ggggatttga
     1261 tagaattaga ttttgttgga gaagcaaaga gaaatcagct tatatataag acttctgaca
     1321 gcgaattaat ggatagagta caagctacat ttcagcaaga taaagagttc ataaaaacta
     1381 atatacgtgc aaaagtgagt attaaactag gtaaaaagcc agagcttttt ataagtgatt
     1441 tttgtggaaa taaatcggtg gtagaaggag acaaaatagt tgaaaaagct ataaaggtgg
     1501 ctttaagtaa agaaaaaata gaaacacaaa ttcaaaagct tggaaataca ccatataagt
     1561 taaaagagat agaaattgac ctggatgaag gtgtaagtat gcctataagt gtgttaaatc
     1621 agatgagaag ggattgtatt gagcttttag atgaagaaag aataaagata aaagatagaa
     1681 aatttaagaa gaatagatta aaataccagc caaatagagt aaataaacag aaaaaacttg
     1741 atacagctcc taagattaga gttaaagtta agaacttgga gcaattagaa gcagtattaa
     1801 attataatgt agatttaatc tattatgaag atataaatac attatcaaaa gctttacaaa
     1861 tggtctcaag tatttctata gattcaaata tttctataga ttcaagtatt tctatggatt
     1921 tagatacttc ttcgtcttta ggtactccta aagaactaat gggttcaaat acaaataaca
     1981 tagatacttt gtcaaataca agtaaaatag atactttgaa taagaaccac tataatacag
     2041 cagtcactga caaaaaatta atctattcag caccaaggat tgttagaaat aaagagtaca
     2101 agcaatttga gatattggat aatataaaag gaattaataa ggttcaaatt ggtaactggg
     2161 gctctatgga atactttaag aaaaaagact taaatataga ttgctactta aatgcattca
     2221 atagtgaaag tataaatcac ttcaaagaag agggagcaag tacagtttgt ttgtcacaag
     2281 aactaaactt aaatgaaatt aaacaaactt taaaatatac agatgtagaa attgaagcag
     2341 taatatatgg atatgctcct atgatggtta cagaatactg tcctatggga gtagttgtta
     2401 gagattgtaa aaaggacaag agagttgcta aatgtaagga aagtagttat gctttaagag
     2461 actttacgga tgcaagttat aggataacac aagatatatt ctgtagaagt acaatttaca
     2521 attcgaaggt tacttgtatg ttggacaatc tatatgagtt agaggaaatt ggaatagatg
     2581 tatttaggat agattttaca gttgaagata gtgagatggt taaaaaggtt attgaagctc
     2641 acattgaggt attgagtaat aattataaac ttggaaaaaa ggctacagac ttgtataaga
     2701 aattagatga aattggaaca gttgcaggac actactataa aggtgtagaa tagatataac
     2761 atgaaaaata tacatgaata tatataaaaa cttgaattta tgtgtattaa tatataaaaa
     2821 tatcatatat gtgaaaaaac agggggattg gtgaaaatga gagaagaagc tttagaaatt
     2881 ttatttaagt atttagaaac tgatagaatg agaaaacatt gttatgctgt tgaagctgtt
     2941 atgagagaat tagccaaaag actagagcca gaaaaagaag aagagtgggc agtggcaggt
     3001 cttttacatg atttggattc agatatagtt ggaagaaagc caggagaaat atgtgaagga
     3061 catgctacaa caacagtaga actgcttaaa aaagaaaact tcggtgacga agaaatgtat
     3121 agagcaatac ttggtcatca tgatggaatg ggagttacaa gaactagcct aatggaaaaa
     3181 gcaatatatg cagcagaccc tataactggg tttataactg ctatagcttt agtttatcca
     3241 gataaaaaac ttaccagtgt aaagactaag tctgttatta aaagaatgaa agaaacgaga
     3301 tttgcttcaa atgtaaatag agatgcaatg agaagtatag aagatatagg gattccattt
     3361 gatgaatttg cagagctttc tcttaatgca atgaagaata taagtgatgt gcttgaaagt
     3421 gctgaaaata attagttgag aatataaaag agaaagcaag gcgagataca tgaaacttac
     3481 aactatttgt tatatagaaa aagatgacaa gactttgatg ctgtacagaa ataaaaagaa
     3541 agatgatata catgaaggta agtatgtagg agttggagga aagtttgagc aaggagagac
     3601 tccagaagaa tgtgtaatta gagaagtaaa agaagagact ggtcttactc ttaaatcatt
     3661 gtcatataaa gggttgataa cttttccaaa atttaaagat gaagaagatt ggtatatgtt
     3721 tttgtatttc tcagatgagt ttgaaggtga attatctgaa aaagatttaa atgattgtaa
     3781 agaaggaaat ttaatttggg ttgataatga taaaatattc gatttaaata tgtgggaagg
     3841 tgacaggctg ttcttaaatt gggctaagac tggaaatatc tttagtgcaa agattgtata
     3901 tgataatggg aaactaaaag attataatgt aagtttctta gattaattaa gatatagttt
     3961 ttagattaat taaaatgtag tttaatgata tattttaaga atataattta aagatactac
     4021 ttaaaaatgt aatttaaagt tacatcaaat taaaatgtaa cttacaggtt atagcaaatt
     4081 aaatttattg atataatatt aaaataaaca atagtagttg ggaaaagggg ctatctcagt
     4141 gcataaagat agcctttttt tagtctgtct acttgacagg gacatatcca aaatagaggt
     4201 aagccttttt tattgttgaa atgataaata tccacttgat ttcatacaat aaagaatatc
     4261 taattaacta aaaagttcac ttttagacat ctacttcttt tatcttttac tttgaataag
     4321 ttctaattta agttccaatt tatgtatatt tttctccatt aaagtgtagt taaggctagt
     4381 atattgttaa aaaaataaaa attgacttag acaactattt gttttatact aaagtaaatt
     4441 ttttaaaagg aggaataatt tggaatgata gacataaatt tagaaaaatg tgtaggctgt
     4501 ggtatgtgtg aatcagactg tcttgtaaat gcaataaaag ttaaggatga taaagcaaag
     4561 gttaaaaata ttctctgtat taactgtggt cactgtatgg caatttgccc aacagatgca
     4621 atagaaatgc aaggatttga taaaaatgaa gtaatagaat acaatcgtga aagttttgaa
     4681 ttagaacccg aaaagttatt gaattttatt aaatttagaa gaagtataag acaatataaa
     4741 gatatagagg tagaagaaga aaaaataaaa aacatagtgg aagctggtag atacacacca
     4801 acaggtggta atagacagcc tataagatac atattagtaa aagaaaagct aaaagaagtg
     4861 aaagaactgg caatacaagg tctttataat ttggcattag atactgatga caatgaccct
     4921 gtacgttcta tgtataaaaa tacatttaag aaaatgtaca aaagatacaa agagaacgga
     4981 aatgattctt tattttatga tgccccacta cttatggtag ttgtaggtga tatgtctcta
     5041 ggtggaagtg cttatgtaga tggaggattg gctgcatcta atatggaact tatggcctat
     5101 tctcaaggat taggaatttg ttataatgga ttttttgtta tggcatcaaa tgtagagcca
     5161 aagataaaag aactgttagg aatgagcgag aacgaagctg ttatcacttc atttatattg
     5221 ggttatccag atgtgaaata taaaagaact gtaaatagaa acactgctaa atttgaggtg
     5281 agatagaact taaacttcac tacaacttgt aaaattatgg gatatagagt aggtatttag
     5341 cagggtaaat ttgttgtaat agacatataa atgagttata attta
//
LOCUS       DAEOSP010000219         5372 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.223, whole genome shotgun sequence.
ACCESSION   DAEOSP010000219 DAEOSP010000000
VERSION     DAEOSP010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.223"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            238..1701
                     /locus_tag="KRH54_003197"
     CDS             238..1701
                     /locus_tag="KRH54_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBG8580828.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHTQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            1757..2893
                     /locus_tag="KRH54_003198"
     CDS             1757..2893
                     /locus_tag="KRH54_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBG8580829.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            3129..4244
                     /locus_tag="KRH54_003199"
     CDS             3129..4244
                     /locus_tag="KRH54_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBG8580830.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTIMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLDPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            4396..5265
                     /locus_tag="KRH54_003200"
     CDS             4396..5265
                     /locus_tag="KRH54_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG8580831.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWLLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT         2067 a          549 c         1006 g         1750 t
ORIGIN      
        1 ttatattgtt tgattattat tcaaacacac gtcgttaaaa attttataaa tttatgttta
       61 gatatgatta atactcttga ttttagaaat aaatatttgt agattgtaga ttaaagataa
      121 tataaataaa tagaaggtag attttttaga tatattaata taaagattat gtaaaatatt
      181 gaaatatagc tgtttaaatt attgtctata aatataaaat gaatggtggt gagttaagtg
      241 accgatttag ttaacaccat taatggaatt gtttggtcac ccgtattagt tgcaatgtgt
      301 ttgtgtatag gattatattt ttcattgaaa ttaaaatttt ttcaaataag agatgtaaaa
      361 gaaatgttta ctctcttgct tgaaggagat agttctcatg ttggtatatc atcatttcaa
      421 ggttttgcta catctatggc tggaagaata gggactggaa atatagctgg tgtagctgta
      481 gcaatagcaa tgggaggtcc aggagcatta gtatggatgt gcataatggc tacattaggt
      541 tcagcaactg catttgtaga atctactcta gctcaaatat ataaagatga gcatgatggg
      601 cagtatagag gaggacctcc atattatttt gaaaaaggtc ttgggtggaa aaagtgttca
      661 gttttatttt caatagttgc aataataagt tatacatttt ttatgcctgg tacacaatca
      721 aacactttat atctagcaat gaatcaagca tttggatttt caaaaatgac aatagcagta
      781 gcatcttcta ttgcattagc gataataatt tttggaggaa ttaaaaggat tggtttattt
      841 gctgaaaaag tagtacctat aatgggtgga gcatacctta ttatgacatt agtaataatt
      901 ataacaaata ttagtttagt tccagaggct attgtaacaa tgtttaaatc tgcatttgga
      961 tttggttcag tttttggagg aatgctaggt tcagcaatat cttggggagt aaaaagaggt
     1021 atatactcaa atgaagcagg agaaggttct gggacttatg gtgcggcagc agcagaagta
     1081 agtcatccag ccaaacaagg attagttcaa gcattttctg tttatgtaga cacattatta
     1141 atatgtatgg ctacaggtat tatgattgta ataacaggaa tgtataatgt tacaggtgtt
     1201 tgcacagcac taccaggtgt agaaccggga ccacaatatg tacagagtgc aataaataca
     1261 ttacatacac aattaggtga tatttttata gcaatagttt tattattatt tacatttaca
     1321 actttattag ccttctcata tatgatggag actaatgtat cttacttagt aactagaatt
     1381 aaagcagaaa aacatgaaaa gtacataatt aatgtctgta gatttatgct tatagcatgt
     1441 gcagcaatgt gttgttttat agaaccactt acaagctggg cattaggtga tatatgtata
     1501 ggtgtattaa catatataaa tttaattgcc atctttacac taaaaaaacc agctataaag
     1561 gcatattatg attatataag acagaagaag atgggaataa aaaggagtga tagaactttt
     1621 aaccctatag agttagatat aagaaatgct gagttttggg aaaatagata cttaaaagag
     1681 gataagcatg agttaaatta aagttataat aaaatataaa aattactaaa taaatttagt
     1741 gggaggtaag attggtatga aagaaagaat aatacaagtg gctgattcaa agaaagaaaa
     1801 aattctaggg ctatgtcaat ctttatatga tgaaccagaa attgctttgc aagaatataa
     1861 atctgctaaa aaaatatcag aatttttaag agaagagggt tttgatgttg aggaaaactt
     1921 ggcaggtatg gctacagctt ttaaagcaac caagaaaaat ggagatggtc ctaaaatagc
     1981 atttatagca gaatatgatg ctcttccagg caatggacat gcatgtggac atcatttaat
     2041 tgcatctatg ggagttggag ctggaatagc tctttcaagt atactggata cttacaaggg
     2101 agaagtaagt ataattggaa cacctgctga agaaactgga gatggaaagc cttacttgat
     2161 agaacatggt gtatttgatg gctatgatgc agcaatgatg atacatccta attcaaaaac
     2221 ttgtgtaaca cctgaaatta tagcaatagg aggtctagat ttcatattta ctggaaaagc
     2281 atctcatgca ggagctaagc catataacgg aataaatgct ttagatgcag tagtactact
     2341 atataataat ataaatgcac taagacaaca attagtagat ggaacgagaa tacatggtat
     2401 tatactagaa gcaggaactg ctgcaaatgt aatacctgat atgggaaaag taagattaga
     2461 gataagggca aaagaacaaa attactttga tgaagtagta gaaaaagtga aaaattgtgc
     2521 tagaggtgct gcaatagcca ctggatgtga acttgaattt tatcactttg aaccaacttg
     2581 ccaaggatta aatgaaaata aagttttggt agacatattt acaaagatta tggaggaatt
     2641 tggaatatat gaagatgaac aagtaatgtt aggttcaaca gatatgggaa atttaagtca
     2701 aataatgcca tgtatacatc ctttgatgaa gttttctgaa aatggagaag aactacatac
     2761 aaaagaattt ttggaggctt ctataaattc atatgcaaaa gatagagtaa ttgatggtat
     2821 aaaaatatta gctttaacag gatttaattt atttgaaaat ccagagttat tgaaaaaaat
     2881 gaaggaagag tagaacttag actattttta attatactct ttaaaataag aagagtgtct
     2941 caaaaggaac tttttagttt atgagacact cttttttgta tgaaattaaa tatattttta
     3001 ttattttaaa catgaaaaag aggcttatct gtttttatat gacctttttt aacttgtagc
     3061 acaaaatatc atgaatgatt acaattcgaa tgataaaatc gattataaga tatataggag
     3121 gggtacatgt ggaaacttta gaagtaagaa atattgaatt gagtggtaca aattatgaga
     3181 taggatacag actaggagaa ttggttgcta atatgccaga gataatagaa ggtcagatta
     3241 ataaatctaa tctggtaagc aaaaaagaag aaaaagagat gatagagttg tttgataaat
     3301 attgtccagg tcttaatgaa gaattgcaag gttttgctga tgcaattcaa gtgaattgta
     3361 atcaaatttt atattatact atgacttatc ttaaaccagg ttgtagtcaa gttgcattag
     3421 caccagaatt aactgaaaat gggcatgtac tatttgcacg taatttcgat ttctcacaca
     3481 atatggagga ttttgtttta tgtaaaacta aggtaaatgg aaaatatgca catataggta
     3541 ctaccattat gcaatttgga agaggtgaag gtatgaatga atgtggctta ggtgtaagtc
     3601 agtcatcatg tggtattcct gtaggcaatt cagatggttt aagaaaacca gcaattgtag
     3661 gtttacagtt ttgggctgtt attagatatt tgttagaaaa ttgcaaagat gtagatgaag
     3721 cattagaata tttaaaagat atgccaattg catacaatat taatttattg cttgcagata
     3781 agtctggaaa cattgcactt gtggagactt tagatgggaa aaaagaagta aatataatca
     3841 acagtttaga aagtaaaaga gaatattttt tacactctac taatcataca catattgata
     3901 aacttcataa attagatcca caatcaatga aaaattctat tcatagatat aaattaatta
     3961 aagaatatat aaataagtct aaaaaaattg gagagaaaga gttaatgaat ttattgtctt
     4021 caaaatatcc aaatggctta tcttgtaatt actataatga tttttttgga actttaaaaa
     4081 gtatagttat ggatttaaat ataggaaagt ttaatatttt atggggtgga ctagagaata
     4141 aatgggagag ctactattta aaaaatgaca ttaaatctat aacacagagg ataaacataa
     4201 atattgaaaa agcaccatct gacttttttg atttcattga ataaaaaatg tattcaatta
     4261 ttctaaataa ttaggatata taaatcaact aaaagttttt atttatcgta ttgaattagt
     4321 tataatattt tagtatagta aaaaatagtg ctattttaca tatttaaaga gtttaagtag
     4381 aagagaggtg ccatattgga ttacttaaag tattttgaaa aagctgtaat gtatattgag
     4441 aataatttac atgaaaatat aactgtaaat gatgtggcaa aagaaactgg atattcttat
     4501 tatcatttaa cacgcttatt taagtctatg tttggtgaga gtgtaggaag ttatataaaa
     4561 aaaagacgat tagtaagtag tacaaaagaa cttttatata gtgataaaaa agtgatagat
     4621 atagctattg gtagtggatt tgaatcttca gaagcattta gcagagcatt taaatctata
     4681 tacaaggtta gccctataga atatagaaaa aatagaattg atgtatttgt tggtaaaaag
     4741 aaaaaattag agttagattt tatgagacat cttgttggaa atataacgat taaaccagtt
     4801 ataaaagaaa tagaaaaaat aaaggtaata ggtataaagg ataaagtaat tttagaaaat
     4861 aattcattac ctgacttgtg ggagaaattc agaaaagtac atcatattgt cccaaatact
     4921 ttgccatcaa agagagtttt tggaatctgt gaagcaatct ctgaaataca cctagttagt
     4981 gaaagtatgg agtttaatga gataatagga cttgaagtta atagttatga tgtgattcca
     5041 aattcatttg tttcaaaaac tattaaggaa ggaaagtatg ctatatttac tcatactggt
     5101 agcttagata atttagacaa aacttatgaa tatatatggg gtacttggct tttaagttca
     5161 aaagaagaac ttgatataag agatgatttt gaagtatatg atgaacgatt tttaggtcct
     5221 agtaatgtga actcacaaat tgatatttat atacctataa aataatctat aagaaaacta
     5281 aaagataatt tgattaagaa atagatgttc gtaaaagagc atctattttt tatttttatc
     5341 agatttagat aattttaata ataaaagaaa ta
//
LOCUS       DAEOSP010000220         5359 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.224, whole genome shotgun sequence.
ACCESSION   DAEOSP010000220 DAEOSP010000000
VERSION     DAEOSP010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.224"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(176..784)
                     /locus_tag="KRH54_003201"
     CDS             complement(176..784)
                     /locus_tag="KRH54_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBG8580832.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISCQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            complement(1044..1589)
                     /locus_tag="KRH54_003202"
     CDS             complement(1044..1589)
                     /locus_tag="KRH54_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBG8580833.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(1707..3530)
                     /locus_tag="KRH54_003203"
     CDS             complement(1707..3530)
                     /locus_tag="KRH54_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580834.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLLIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KSGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(3543..5318)
                     /locus_tag="KRH54_003204"
     CDS             complement(3543..5318)
                     /locus_tag="KRH54_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580835.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQNFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         1802 a          942 c          467 g         2148 t
ORIGIN      
        1 acactatcta atattagttt cttataagtc ataaaagcac aactaacact ataattctag
       61 tgattaattt tatttcaaaa aatataataa cactactaat attttaaata tcaatagtgt
      121 cataaaaact tttcagatta attaaatctt tctatatatt ttataatagt ctaaatcatg
      181 ctaaatattt agacagaaaa cttttttctt taatagtaaa tacttttgtt tgaatatcag
      241 aataattatt tgaatatcta tctataaaac catgatttgc atctaaaatc tcaatttgga
      301 tattttgttt cctagaaagt tggcaagaaa tagaatcaac atcaaatgaa tcgtgctttg
      361 caaatactaa taaggtagga cattttggaa ttacatccat gtaatctcga atccgagagc
      421 cataacaaca aattatgcca tcacaaaaag aatcttcact gcatctccat gcaatagttg
      481 ctcccacgct atacccagca acaaatacat atttatactc tgattttaaa tcatttataa
      541 gcttttcaac ttttttataa atatcaaaac caacattatt tataaaaaaa tcatatgcct
      601 cttggacttt tgaatatgga aaaactcttt tttctcccag aagttcagga caaaatacat
      661 catatccata tttataatac ttttgacaaa catcttctat aaagctatta atcccataga
      721 tttcatgcaa aacaacaata gctacatcac ttttatgtat atactttatc agattatcca
      781 ccacctttac aaaacagcaa attttttata cttagtttaa tatattcttc tttacatgtc
      841 aatttacttt tctaaaatca aatttatttt tggattaatc atttattttt aaaagttgtg
      901 cattattact attaaattat tctctattat taaaaatata atcgttagaa ataaaaaata
      961 aagtacaaat aattaaaaaa tatctcactc ataactttaa aaattataag tgagatactc
     1021 ctaaaatcta ctttatatta aatttatttt ccaaaatatc tatttaataa tcctaagaat
     1081 gctttaccat gtctagcttc atctttacac atttcatgta ctgtatcatg tatagcatct
     1141 aatcctaatt cttttgcttt cttagctaat tttaattttc catcagttgc accatactca
     1201 gcatcaactc taactcttaa gttttctttt gtatctgcaa ctacaacttc tccaagaagt
     1261 tctgcaaatt tagcagcatg ttcagcttct tcaagagcta ttctcttata agcttctcct
     1321 acttctggat aaccttctct atcagcttgt ctactcattg ctaaatacat tcctacttct
     1381 gtacactcac caacaaagtt agctcttaat ccttcgatta tttcttcatc tacaccttga
     1441 gctactccta ttctgtgctc atcagcccaa tttttttcgc ctttcatttc ttcaaattta
     1501 tcagctccaa ctttacatac tggacattgt gctggtgcag catctccttc atgtatatat
     1561 ccacatactg tacaaacaaa tttcttcata attactttcc tcctaaattt tatatatcaa
     1621 attaaatttt ttatttatta agttagtcta tatatacccg caccatatac ctttaaacaa
     1681 tttttttaaa agttttttct aaatacttat tttccctgtg tcaataatat gtttctataa
     1741 taatgtccct ttaattttat caattcttta tgatttcctg attccacaat tctaccattt
     1801 tctaaaacta ttatattatc acaatttgat atggtactta atctatgagc tataattata
     1861 cttgtctttc cataagttaa attatttatt gcttcttgaa ttttagcttc tgttgctata
     1921 tctatgttac ttgtagcctc atccaaaata agtattggag cattttttaa tactgctcta
     1981 gcaatactta ttaattgttt ttgaccttca cttaaatctt cacccctatc cgataataca
     2041 gtactatatc cttcacgcat tttaactata aatttatgtg catttgctat tttactagca
     2101 tatataactt cttcatcagt agcctccaga tttccatacc ttatattttc cataactgta
     2161 tcattgaata tataaccttc ttgtggaact actccaaaac aagttctcaa atagtttctg
     2221 ttaatagagt atatatcctt accatctaaa aatatacttc cttcatctat atgataaaaa
     2281 tttgtaatta gatttataat tgttgttttt ccacttcccg tctctcctat gatggcattt
     2341 gatgtgccag atttgatttt taggtttata ttatctaaaa ttttcttttt tgaatcataa
     2401 gaaaaactta catctttaag ctcaatttct ccctttacaa aatgagagtt taaattacca
     2461 tctttattat cgttaatctc acttttaaac atgttatgca cttcttgctc ttcttgttca
     2521 tctataattt caaaaactct ttcacatcca gctatggctg aaagtaatgt attaaataga
     2581 tttgctaatt catttaaagg tcttgtaaat tgtcttacat aacttaaaaa actagctata
     2641 acccctatgg ttataatatc tttagatgca agaatgcctc ctataataca tatagttgca
     2701 aatcctatat tactaatcac attcataata ggcattaaaa gagaagtgta aatttgcgat
     2761 ttaagggaaa tatttagaag tctatcattg acttctttaa aatcatttac agattttctt
     2821 tcataattaa aagattttac tacatttatg tttgatatca tttcttcaat atgtgcattt
     2881 agttttccca actcaacttg ttgttgctta aaaaaaactc tagttctctt tgcaataaaa
     2941 ttactcacaa acattactat cggtattaat attacactgc aaaaggttaa aattgggctt
     3001 aatacaaaca tcataataaa agtcatcaat atgtttaaga ttcctgtaat taactgtact
     3061 aatgaatttg atattcctgt actgatatta tcaacatcat ttgtcactct actcataata
     3121 tctccagtag aacttttatc aaaaaaggtt attggtagat ttttaaattt taaaaataaa
     3181 ttacttctca tttctttaac tatactttga gataatctag ctgtacatat ccccttagac
     3241 agtttaactg aaaactcaat tatataaaat gaaagtaaaa taattattcc ttttaagaaa
     3301 atggattttg aataagtatc gatatctatt atgtcaacta cctttccaat agtatatggt
     3361 attacagttg atataattga atcaattatt aatagcaaaa aaataaatct agtaatcttc
     3421 ttatcttttt taaagtacat aaaaattctt ttaaacgttt ttttaaaatc tttaggcttt
     3481 tcttttttct ttaatctact gaccttctga tttccagtac ttaaattcaa tatacttcct
     3541 ccttaattta catcttcact tactaaattt tgcaacgcaa aaattttttt ataaattgga
     3601 gaactaacca gtaaatcttc atgtgtagca aatccaacca cctcaccctc atccaagact
     3661 aaaatcttat cacagtcaat tatagaacta atcttttgtg aaatagttat tacagtacat
     3721 ttcttacttt ccttttctct taaattactt atatactctt ttatattctt tcttataatt
     3781 gtctctgtat taatatccat tgcactaaaa caatcatcaa gaattaatat ttgtggtttt
     3841 ttggctatag ctctagctat agtaattctt tgtctctgtc ctcctgataa atttttaccc
     3901 ctttggaata aaacttcatc ataagtattt tcgagttttg ttataaattg atttgcattt
     3961 gctatttcac atgattttac aacatcttct ttgcatatat ctctatttcc cataactaca
     4021 ttctccaata tactttttcc aaacacacta ctattttgaa atactacacc tattcttgcc
     4081 cttaaacttt cttcagaaat actgtatata ctacgtcctc caatcttaat atcaccttta
     4141 tcaattatat ataatttttg aattaaattt actaaagaag tctttcctga accagtttga
     4201 ccaatcacac caatattttg accttcttct attttaaagg atacgttttt taatattttt
     4261 tcatctgtat ttttgttata cgaaaaatct acattttcaa aaattatatc acaatttaca
     4321 aactttaaat ctttttctcc aacatctaaa ctttcttctc tctcaattac ttcttcattc
     4381 tcatcaacca aaacctcact tattctctca taagatgctt ttgccctaac aaaaacatta
     4441 aaaacatgtg aaatcattat aagagatgtt aatattctag tcatatagtt tacataagca
     4501 actataatcc caattttaat ttctcctgaa taaattaaat gccttccaat tagtaatatt
     4561 attattatgg aaacattaat gattattgta attgtcggtc taaaaaatga catcatagtt
     4621 gtagcttttc tactattttt aaaaagttca tcatttaact cttcaaagct ttggttctca
     4681 taatcgtatc tacaaaatag tttaactact tttattccac ttaaaaactg tcttaatttt
     4741 gaatttactt tatctgtact ttcttgtaca tttttaaata atctataccc tatcttcata
     4801 ttaagcaata ttataataaa tataattaaa actgcaatta gaataattat tgacatcttt
     4861 aaatttaatt ttatagctat aattaaactt cctatacaca ttattggtga ctttataaaa
     4921 actctcatca ttccattaac gaattgttgc atttgattga tatcattagt aattcttgtt
     4981 attaatgaag ctctaccaaa tttatctatt tgttttaatg agtatgtatt tatcttttca
     5041 aatacatcct gtcttaaatc ataagaaaaa ttctgagaaa ctcttgccga gattacactt
     5101 cttattattg caaatattgc acctaaaaaa gttataccta tcattatagc tcctgttgat
     5161 agtatgtagt ctaagttctt atttgaaacc cctatatcta ttatttttgc cattatagtt
     5221 ggttgagcta aatcacaaaa agcttccaat gttaaaaaaa atactgctgt tagaaataat
     5281 actatatatt tttttatgta ttttttaaaa tacctcattc aatcatccct tcatagaatt
     5341 ttctcattac caaaagtat
//
LOCUS       DAEOSP010000221         5352 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.225, whole genome shotgun sequence.
ACCESSION   DAEOSP010000221 DAEOSP010000000
VERSION     DAEOSP010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.225"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(204..1388)
                     /locus_tag="KRH54_003205"
     CDS             complement(204..1388)
                     /locus_tag="KRH54_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBG8580836.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMVKLVNAVPVFIDTKKENGFKLTKDELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1761..4112
                     /locus_tag="KRH54_003206"
     CDS             1761..4112
                     /locus_tag="KRH54_003206"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBG8580837.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4134..4673
                     /gene="nrdG"
                     /locus_tag="KRH54_003207"
     CDS             4134..4673
                     /gene="nrdG"
                     /locus_tag="KRH54_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBG8580838.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2097 a          598 c          851 g         1806 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttacct tatatattaa ttaccattaa tacattatat
      181 ctaattacta ttcatctaaa ttattatgcc attattttat atacaaattc ttttaattta
      241 tctaatccag ataagaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttca tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa ccatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcat ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatttgtag atatatacat taatatatat aatagcctac actttttgcc
     1501 taaaattagt gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgcta
     1561 ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca
     1621 aatttggtaa tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga
     1681 tttttttaga cttaacatac tacatatagt ggtatgggga ttaataaaat acaatatata
     1741 gtaacaggag gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga agagtaattc
     1801 catttaatga agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa
     1861 gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt
     1921 ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag
     1981 ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa
     2041 ctgaaagaag tagaataaga aattcaaaaa caagattgat gaaggcaata gaggaaatta
     2101 cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata
     2161 cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac
     2221 atatattaaa accagagcat tcttttgctc atgataatgg agatatacac atacatgata
     2281 tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca
     2341 atggtggatt ttcaacagga catggctttt taagagagcc acaagatata ataagttatg
     2401 gtgcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta
     2461 taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca
     2521 taggaaattt agctaaagca ttgaagttat ttaaaggaat tgaaaataat gatgtgataa
     2581 agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg
     2641 aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac
     2701 ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa
     2761 catatcaatc tatggaagca tttatacaca atctaaatac aatgcactct agagcaggtg
     2821 cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg
     2881 taactaagaa tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt
     2941 ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt
     3001 atgatttatt taaattatca tgcagagtat cagctaaaag actattccct aattttagct
     3061 ttttagatgc gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaggcta
     3121 catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg
     3181 gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata aaacatggta
     3241 ttataaataa tgaaaaggct aatttagatg gtttctttga agaattagat gagaagataa
     3301 acttaattat agaacaatta ttggagcgcc ttgaggtaca aggaaataaa aagatgaaga
     3361 attttccatt cttaatgggg caaggtgtat ggaaaggttc tgatgattta ggaccagaag
     3421 atacactaaa agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag
     3481 agtgccttat tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag
     3541 gattaaaaat agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat
     3601 taaatttctc cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag
     3661 ataaaaaagt ttatggagaa attaaaggta tcacagataa agaatattat actaattcat
     3721 tccatgtacc agtttattac aatataagtg catatgataa aattgagata gaagctccat
     3781 accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg
     3841 ataatttaga agcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg
     3901 gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa
     3961 gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgacatcaaa ttcgaaagaa
     4021 taagaagaat aacaggatac ttagttggta cagttgatag atttaataat gctaaaaaag
     4081 cagaagtaag agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa
     4141 taagaatgtc atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg
     4201 taatatggac tcaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg
     4261 atttatgtgg aggattttat atggatacag aagaaattat aaataaagtc aaatcattga
     4321 aattacaaaa aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag
     4381 aagaaatagc aagagaagct aaaataaatg ggttagatgt atggtcatat actggattta
     4441 cgtttgaaca actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa
     4501 agcaaattga tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa
     4561 agtttagagg gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaaa
     4621 aagtgttttt ggttgagcaa tatatgaagg atgatttatc tatagcagaa taaaatataa
     4681 ataaacagga ttaaatatat gcatatattt aatcctgttt attttcatat aatatttcaa
     4741 gcttgtataa tgtaacaatt aaaccaaaag actcattgta tatttgaaat taagtttttt
     4801 atagtttata ggtgttttat gtggattacg aatgatatag tattgataca ttatttaata
     4861 gattcttatg ccaagaaaca attaatttat agaaattctt aattgttaca atataaatat
     4921 ctagataagt tataagatac tgataaataa tttataaagt ttattaatct aatattgtgt
     4981 gtgaatatta agtggtcaag tagtctaaaa aatgaaaaag atttaaaaaa gtgttttaac
     5041 taaacacata agtgttaaat tcatgtataa gtataggtta gatacataag aaataattat
     5101 gtaatatcta tctttgtaaa aaaatattaa attttgtaat ttgtggataa atatgtttat
     5161 aatgtggata atatttaatg taataagtta aagcaatatg aatcaactgt ttataatgtg
     5221 gataatatta taaatgtatg aataattgca gtatttaaaa acaaaaaaag ttaaaaaagt
     5281 tgttgactct aaaagaaaaa gttggtatag taatacttgt cctcaagaaa agggcaaaca
     5341 aataaagcat aa
//
LOCUS       DAEOSP010000222         5351 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.226, whole genome shotgun sequence.
ACCESSION   DAEOSP010000222 DAEOSP010000000
VERSION     DAEOSP010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.226"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(222..2501)
                     /locus_tag="KRH54_003208"
     CDS             complement(222..2501)
                     /locus_tag="KRH54_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG8580839.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2772..4226)
                     /locus_tag="KRH54_003209"
     CDS             complement(2772..4226)
                     /locus_tag="KRH54_003209"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBG8580840.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4390..5259
                     /locus_tag="KRH54_003210"
     CDS             4390..5259
                     /locus_tag="KRH54_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8580841.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDATSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
BASE COUNT         1828 a          836 c          567 g         2120 t
ORIGIN      
        1 actatctaaa gtattttgct ataatcccat atactcttac ctcaaaaaca tccaaatgta
       61 aaaaatacta catattaata aactttgccc aataattcct aaaataatat cctaacaatg
      121 atttatgttt ttataatagt atatttttac ataaaaaagt gaacattgat taacaaagtt
      181 cactttttta taaattttta tgttataatt cacaatttta tttaatttgt ttcttttcta
      241 tttaaactat atctatctaa tattaatttt tcatattctt ctattggcat tggtctagca
      301 aataaaaatc cttgggccat atcacaacct ataaacctta aaaactctgc ttgctctaca
      361 gtttcaacac cttctgaaac aacttttata tcaagttgct ttgccatttg aacaatactt
      421 gaaactacaa tttgactctt ttttgcattt cccactgtta taaagaattg cctatctaat
      481 ttcaatacat caattggcat atctttaagc atatttaaag aagaataacc agaaccaaaa
      541 tcatccatag aaattttaaa tccaacagat ttcaaagttt gcattatttt aaataatatt
      601 tttgtattgt caaatattgc actttctgtc aattcaatct caattaaatt tggttcaatt
      661 tcatattctt ttgttattct ttcaagctca gaaataaagc ctggattctc aagatgtact
      721 cttgataagt ttattgatat aggaaatgtg gggaaatttt cattttccca acgtttaaaa
      781 tttttacaaa cttgggtaaa tacaaacata tccaattctg ttataaaacc atttttctca
      841 aaaataggaa taaatacacc tggacttatt agaccttttt taggatgttg ccaacgtaca
      901 agtgcttcag cacctacaat tttagtagtt gataaatcaa ccttaggctg taaataaact
      961 ttaaattgtt cattttttaa agcatcatgc atttcttgtt caatatcacg ttcttctatg
     1021 agagtttctt ttaaattttc agcataaatc tcataaactt ttaaatattt tctttttact
     1081 gtagtttttg caatcatagc tttgtcaaga caggtagaaa tatttgtttc tccctcttca
     1141 acaaaatata cacctatgga tggaattaaa ttgagttcta ctcccaaact tgtttttatc
     1201 attgacaatt ttttacgtat aagttcacat atttctataa ttgactctct attatttttc
     1261 ttttcaaata ttatagcaaa attgtcattt gaaattcttg aaaatacaga ttgctcttta
     1321 aatatattct taattatctc tgctatttcc tttaatactc tatctccttc ttcataccca
     1381 aacctatcat ttactagctt aaacttatct atatcaaaat aaattaaaac tagattcttt
     1441 ttctcatgag aggataaaaa ttttccccct tcaagattaa atttatttga attgccaata
     1501 cctgtaacgt tatctttaaa agcaaattct tccaattttt ccttactctt tttatgtata
     1561 aatagtatat aaattgttaa tgccgtaaag attataatca agactacaca tgctgataat
     1621 gtaagtttta taatagcata tgatttctca gatatagcag ttttaggaac ttcagaaaaa
     1681 agataccaat cattgattcc tattggggca taacttaaat aatatccttt tccaaacata
     1741 ctataactta atgaacctga tttaaagttt tttatatttg acttcatatt gttgatacta
     1801 gattttgata ttgaatcact tctgccaatg taagaaaata aattttcttt agtagagttg
     1861 gcgtttttac tgcttggatt taaaacaata tcaccattac ttctagttat ataagaatat
     1921 ccttttccat taaatgtagg tacagaaagt attttttcat acaacttcgt actatgtgtg
     1981 gcaaatataa ctcctttaac ctcattatct ttataaattg gtacagaata tacattaata
     2041 ttactattgg tatttttatc atcttctatt acatctttta atttgcctgt tataaccact
     2101 tctccttgca ttgattttaa gaaataatct cttttagaaa agtcttctat ataaccatct
     2161 gtagtgtatg ctgtaccatt tggtaaaata actcccattc ttttaaatga actattaata
     2221 gcttgccttt ttagtataga aaggatatta tctacttcaa aagactcttc tccttcaata
     2281 aatattgaaa ttgactctag taactttata tctccattaa tttgtttttt taacacattg
     2341 acgctttgat ttgctatttc tgaaagatat tgattcgttt cttcacctaa tgtcttattc
     2401 aaccttgaac cataaatcaa acaggaaatt gtaattacta agatacttat gacagcaatt
     2461 atggacaaac catacctatt attttttgca tcacttaaca ttatgcttcc tgcctttctc
     2521 gaatctatgt atgtataatc attatcttat gacaattatt gtaaacattt tataataata
     2581 cttatttttc catatttact gtaattatac aaaattataa actttattac aacaataaat
     2641 taattaatat agatattagt gtttatatta ttgcattatc tattttacaa ttatttacat
     2701 ataaaaaaat gtaatatcaa agtgaagctt gatattacat ttttgttctt gtattttgtt
     2761 taatttttat gctacatctt acttaaacac tcatttgcaa cttctttatc atcaaaatga
     2821 tgcttaactt tacctataat ttgataagtt tcatgtccct tacctgttat aattacaaca
     2881 tcatcctttt ttgccatact tatagcttct gctatagctt gttctctatc aacaacaaca
     2941 tggtaatttt cttttttctt gtcaattcct tcaagtatat cttttattat tgcctctggt
     3001 tcttcagttc ttggattatc agaagttata atacataaat cagaatattt ttgtccaatt
     3061 gctcccatca aaggtctctt ttcagtatct ctatctccac cacacccaaa cacagaaatt
     3121 atttttcctt ctgcaaattg ttgagttgtc tttagtacat tttcaagtgc gtcaggagta
     3181 tgagcatagt ctacaattac gcttatacct ttatcatttg gaactgtttc aaaccttcct
     3241 gatactccat tagaaagtct aaggccttct ttatatattg gtttaggtat gccaagtaca
     3301 tagcaagcag ctataactgc taaagtatta tatactgtaa acattcctgg aactggtata
     3361 aatattactt cttcatatga aggtgttatt aatctataag atactccatc cgaatctgat
     3421 tttatatctc tagccataaa atcagcctta gtatcaacac cataagtgta acaaggtaca
     3481 tttatacctt ttatattttc atatatcttc tttccacctt catcatctat gtttattata
     3541 tttgccatag tagtcttaaa aaataatttt tcttttgctt ttctataatc ctctaaatct
     3601 ttatgaaaat ctaaatggtc tggtgttaaa tttgtaaata tgcctagttt aaagtctgtt
     3661 tcatcaactc tatttaatgc caatgaatgt gatgaaactt ccattgcaca gtagtcacat
     3721 ccattatcca acatattatt aaaatgatat tgtaaatcta tactttctgg agttgttgaa
     3781 ttggaaacaa tgtctttttc tccatcaaaa atttttatag ttcctataag tccaacttta
     3841 ttcttattta aagttaatat ctcatttagt atagtagtta tactagtttt tccattagta
     3901 cctgtaactc ctataacgtt aaatttttga gatggatgat tgtaaaaatt atcagctact
     3961 tttgccatat cttttcttgt atcttttacc tttataaatg tacatccttt tattgctaca
     4021 tcttcttcaa caagaaatgc acttgctccc ttttctatgg catcttttat atacttatga
     4081 ccatctgaca caaatccttt tatacatata aataatgtac cttttgtaac ctttcttgaa
     4141 tcatattgta cattattaat atctatattt aattcaccct tgacattgat tatatccaac
     4201 ccctgtataa tatcatttaa tttcataaat gctttcctcc ctttaaactc tttctgctaa
     4261 aattacacta catataatat tatattaaga attttttact atgtgaatgg ttaaaaatgc
     4321 ttgaaacatt gcaaaaaatg ctctataatt ttctataata taactgagga aaggtcttct
     4381 ataaaaacta tggataataa aatcgggtac ttaaacgata actttaaaat atttaatatt
     4441 agagataaaa aagatataca atttgaatac cataatcatg attttaataa gataattata
     4501 tttataaatg gaaatgtaac ttattttgtt gatggtattc cctataaact aaagccttgg
     4561 gatatactct ttatcagcaa taatgaaata cataaaccag ttatagattc aaatgtcttt
     4621 tatgagagaa ttgcaatatg gattagccca gaatttatga aaaaaaatag tgatgctact
     4681 agtgacttag aaaaatgttt taaaatagct acagagaata aatgcaatag attgcgttta
     4741 aatatacatg atattgaatc tattaagagt tttattgagc aaataaatat ttccgaatcc
     4801 aatactgaat ttggaaatga aatattgaga aatgcattat ttatacaact tatgatattc
     4861 ttaaacagaa ttttcctaaa aaatgaaaat atggaatcaa cagaagacgt tttttatgat
     4921 gaaactatta agaaagtatt aaactatata aattcaaata tagatgataa gctttcaata
     4981 gatagcatat cgtctgaatt ttttataagt aagtattatc ttatgagaaa atttaaatct
     5041 catactggaa cgtctataca caattatata gttcaaaaaa gattaatttt ggcaaagtca
     5101 ctaataacca atggtcacct tatgagtgaa gtttgtaata aatgtggatt caacgattat
     5161 tctagttttg taagggcatt taaaaaaaac tacggtgtat ctcctaaaaa ttatataaac
     5221 aataaaaatc ctttagatat tactctttta gatgaataga taatttatgt aaattttatt
     5281 ttaataaaac ttgaagtatt agtgtattta tcttactaat acctcaagtt ttttaatgtt
     5341 ttaaaatata t
//
LOCUS       DAEOSP010000223         5304 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.227, whole genome shotgun sequence.
ACCESSION   DAEOSP010000223 DAEOSP010000000
VERSION     DAEOSP010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.227"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            132..1268
                     /locus_tag="KRH54_003211"
     CDS             132..1268
                     /locus_tag="KRH54_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580842.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            1261..2808
                     /locus_tag="KRH54_003212"
     CDS             1261..2808
                     /locus_tag="KRH54_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBG8580843.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            3142..4008
                     /locus_tag="KRH54_003213"
     CDS             3142..4008
                     /locus_tag="KRH54_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBG8580844.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            4149..5291
                     /locus_tag="KRH54_003214"
     CDS             4149..5291
                     /locus_tag="KRH54_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBG8580845.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
BASE COUNT         2003 a          571 c          967 g         1763 t
ORIGIN      
        1 tagcaatatt cagacaaata gtgacctaaa aatcgtactt tgtttacata aataaaatag
       61 tatgatataa ttataaaaac aacttataat attgaagaag gttgcttgtt cgaaaaaggg
      121 gatgataaat tatgatagag ataagaaatg taacaaaaaa aataggaaat aatgttatat
      181 tagatgatat ttctctagtt gtagaaacag gtacattggt agttttgatt ggttcaagtg
      241 gttgtggaaa gactactact ttgaagctta taaacaaatt aataaaacct acatctggtg
      301 agatatatat taatggcaaa ccaatttctc aggaaaatga aatagagctt agaagaaaaa
      361 taggatatgt tatacagaac acaggcctat tccctcatct tacaataaaa gaaaatatag
      421 agctaattcc acgcctaaaa aaggaaaaat ccgttgaaga aattgaaaaa agaacgttgc
      481 aactacttga gatggtagga cttgatagtg atgaattttt gaataagtat ccttcagaac
      541 ttagtggtgg acagcaacaa agaatcggtg ttgcaagagc cattgctact gatgctgaaa
      601 taatattgat ggatgagcct tttagtgctt tagacccaat aacgagaaca tctttacaag
      661 aacaattatt ttctcttcaa gatgaattga aaaaaactat aatatttgta actcatgata
      721 tggatgaggc tttaaagata gctgataaaa tttgtattat gaaggatgga agaatagcac
      781 aatatgatac tcccgaaaat atacttagaa agcctgcaaa tgactttgta aaagatttta
      841 tcggagaaga tagagtttgg gataatccag aatatataaa agcaagagat attatgataa
      901 aaaatcctat tgctgttaat tctactagaa ctgtaactca aggaatagaa attatgagaa
      961 cgagtaaggt tgatagctta ctaattatag atagagcaaa aacattaaaa ggtattgtta
     1021 ctgtaaaaga tatgaaagat attgatgata agtcaatatt acttgctgat attatgtcaa
     1081 gtgagccttt gcatgtaaat gaaggtgata atttagtaga aatactaaat gtaatgaatc
     1141 gtaattcggt tggatatatt ccggttattt ctgatgaaaa taaactggta gggcttatta
     1201 ctagaagtag tttactttct gttttaagtg aacaattctt agaaatggag gtgagtgttc
     1261 ttggctaatt tttttaactt tatactatta caaaaagata agattataga gcttttaata
     1321 caacatatga gtctaactgt tacatctatt ttaatagcta taatagttgg tgttcctctt
     1381 ggaataatta tttcaagaat ctcatctctt agaaagtttg tacttggatt tgtaaactta
     1441 gtccaagcag ttccgtctat ggcacttttg ggtcttcttg tacctatact tggtatagga
     1501 agtaaaccag ccatatttat ggtagtggtt tattcattac taccaattgt aaaaaatacg
     1561 tatataggta taacaagtat tgacccagta gtattagaat cagctaaagg tattggtctt
     1621 acaagaaatc agacattatt taaaatacaa tttcctttag cgcttcctat aataatgggt
     1681 ggagttagaa tatctgcagt tacagctgtt ggtttgatga ctcttgcagc atttataggt
     1741 gctggtggtt tgggatattt agtattttca ggggttcaaa ctgtaaataa taatatgatt
     1801 ttagcagggg caatcccatc atgtataatt gcactgttag ttgattattt atttagtaaa
     1861 attgaagttg cagttactcc aaaaggatta aatccaaaag cacctaagaa gaattatata
     1921 gcacttaaag taatatctgt agtggttgtt gtttcaatgt tgttcgttgt ttttagttct
     1981 agtttttcaa gtaaaaaaga tacaataact attggttcta aagattatac agaacaactt
     2041 atacttggta atgtttatgc tgaattagta gaaaaaaata cgaatcttaa agttaagaag
     2101 aatttaaatc taggtggttc atctgttgcg tttaatgcaa ttaaatctgg tgagttagac
     2161 atgtatgttg actatacagg tacacttttg gtaaatgtaa tgaaacatgc tccgattaaa
     2221 gatgctgatg aagcatataa tgttgtaaaa gatactatgg aaaaagaaaa tcagttaact
     2281 ttacttgacc ctcttggatt taataatact tacacattag ctatgatgcc tgaaactgct
     2341 gaaaagtatg gaataaatac tatttctgat ttgactaaat atggtaaaga atttacattc
     2401 tcaccaactt tggagtttga aaatagagaa gatggtcttg tagggctttc tagagattat
     2461 ggtttaaaat tcaaagatgt aaaagcaatg aatggaagtt taagatatac agctttagat
     2521 aataatgaat ctcaggttat agatgcattt ttaactgatg gtcttattaa aaagtttaat
     2581 cttaagatat tagaggacga taaaaatttc tttgtaccat attatgcagc accattagta
     2641 agagaagata ctttgaagaa atatcctgag ttagaaaaag ttatgaatat gctttcagga
     2701 aaaataaatg aagaaactat gagagaactt aactatcaag tggatgaact tggtaaatca
     2761 ccagaagaag ttgctcattc attcttagtt aaagaaggct tagtataaaa aattaatagt
     2821 ttatatcaaa aatttaaggg agtgtctcaa aatgaacttt ttagttcatg aggcactctt
     2881 ttttatatga aatcaaatat attttatcat ttcaacaata aaaaagaaga ttatctttat
     2941 tttgagacag tctcttattt ttatgtatac caaaaccata cctacgattt ttatttataa
     3001 ttaagtttca aatagaatga acaagtacaa ccaattttga atataaatat agtaaatgtg
     3061 cttatatacg attttattgt ctggttgatt gatattgata gatagctttc tttattgaat
     3121 cgaagggagg gggaatttgt tatggataac tataagaaag tgaagcaagt attgtggata
     3181 attttatttg ctaattttgc agtagcatta ttaaaaatca ttattggtaa tcaaataaaa
     3241 agctatagta tgacagcaga cggatttcat tctttgtcag atggagcatc taatatagtg
     3301 gggcttatag gtattttctt tgcatctaaa ccaaaagata aaaatcatcc ttatggacat
     3361 aaaaagtttg aaattataac cagtttattt ataagtggta tgttgtttgt tatagcaatt
     3421 aagataattc ttagtgcagt gttaagaata gctaatccag ttgtacctgc tataactata
     3481 gaaagtttaa ttgctttaat tattacactt tttataaata tttttgtatg catgtatgaa
     3541 tatagaattg ggactaaact aaatagttat gttttgattt cggattcatt gcatacaaga
     3601 agtgatatat ttgtgtctct aggtgtattg gttactttag ttggtgttaa gttaggattt
     3661 ccagtaataa tagaatctat agttcctatt attatttctg cattcataat ttattcggct
     3721 tatggaatat ttaggccatc tattggaatt ttggtcgata gagtggctgt agatgaggat
     3781 tatataaaag aaattgtgtt tgaatttaat gaggtaaggg atgttcacaa tatcagaagt
     3841 agaggtagca aaagttctat ttatattgac atgcatgtaa tggtagatcc atttataagc
     3901 gtagagcaat ctcatgattt aacacataaa attgagaaac aaatacaaga agaaattaat
     3961 gaaaatgctc aagtaatagt ccatattgaa cctttttata gtttttaact tagttataaa
     4021 atacaagtta taaaaaaata taatttataa aaaaaatatt ataccataaa taatttataa
     4081 aaattattta tggtataata ttttctataa agaaattaag tcttaatata tctaaggagg
     4141 aatttacaat gagatacgtt gaatatggaa agacagggaa aatggtttca gtagttggct
     4201 atggaggatt aaggtttgat ttagaaaaaa gtaatcaaga aaatgctgat ttagtcaaat
     4261 atgcgtatga aaaaggtatt aattactttg atacagcacc agggtattgt gatgacagaa
     4321 gtgaagatat atttggacta gcctttagac aaatgataaa ggaaggtaag accgattttt
     4381 atgtatcaac taaagaaaag ccaaaagcat gtgatacatc agaaaaagct atagaggctg
     4441 taaaaaaatc aatagaacga ttaggcgtat ctaaaataaa tttttatcat gtttggaata
     4501 ttagaaagat ggaacattac gaactagcaa tgagacctgg aggtcaatat gaaggattat
     4561 tgaaatgtaa agaagaagga ttgatagacc atatagtatt ttcatctcat caaccaggag
     4621 atgaggttat agatattcta agtgaaaata agtttgaagg tgtaactatg ggaataaata
     4681 tattaaactt tccatatagg ctaaaaggtg ccaaatatgc tgttgataat ggatatggtg
     4741 ttgtggcaat gaatccatta agtggtggaa ctattccaaa gtataataaa gaactttctt
     4801 ttctatcaaa agatggcgaa agtactatag aatcagcttt aagattcaac ataggaattt
     4861 cacaaataag tatatctttg atagggttta ataaaaaaca agatatagat gatgcatgta
     4921 aaattgcaga cgaaaataga atttattcag atgaggatat acatgatatt gagactagat
     4981 taaataagaa tatgaacgaa atatgtacag gttgtggtta ttgtaaagta tgtccaaaag
     5041 gtattaatac cccagcctat atgctttttt ataatgaaaa gcagatgttt aaaaagagtg
     5101 atgaagaaat gacaaagtta gtttatggct taggacattg gaattacaca atgaatagta
     5161 aggcaaaagc taaagaatgt atatcttgtg gaaaatgtga agttgaatgt actcagcatt
     5221 tacctataat agatagatta aaagaaatta aaaaatggga agaagatgga gctaatagtg
     5281 ttaaagtcta attaatttta tact
//
LOCUS       DAEOSP010000224         5269 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.228, whole genome shotgun sequence.
ACCESSION   DAEOSP010000224 DAEOSP010000000
VERSION     DAEOSP010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.228"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(16..1383)
                     /locus_tag="KRH54_003215"
     CDS             complement(16..1383)
                     /locus_tag="KRH54_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8580846.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(1633..2097)
                     /locus_tag="KRH54_003216"
     CDS             complement(1633..2097)
                     /locus_tag="KRH54_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBG8580847.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(2102..2731)
                     /locus_tag="KRH54_003217"
     CDS             complement(2102..2731)
                     /locus_tag="KRH54_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBG8580848.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKLKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(2765..3652)
                     /locus_tag="KRH54_003218"
     CDS             complement(2765..3652)
                     /locus_tag="KRH54_003218"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBG8580849.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(3681..4919)
                     /locus_tag="KRH54_003219"
     CDS             complement(3681..4919)
                     /locus_tag="KRH54_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580850.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
BASE COUNT         1948 a          837 c          552 g         1932 t
ORIGIN      
        1 tttatattgt atatcttatt cttttattgc tttaggtttt gatagaaaac atttataaaa
       61 tataaagaaa gaaattgctg ttatagtagc agctgttata tctgcaactg gctctgctaa
      121 aattactgca aaaagttttt cattaaagaa tgttgggagt ataaatatta aaggtattaa
      181 aagtacaatc tttctaagta gagctaaaac caaagatatt gttgcttttc caagtgctaa
      241 aaatgtctgt tgacaagcta tttgaatccc aaacataaaa gcaccagcaa aatatatttt
      301 tatactccaa ctagtcatac taagtaactg cggattctta ttaaatatac taacaaaaaa
      361 tgcaggaaat atcattaata aaagccataa aatagtagaa tatgtaaaac atgacaacaa
      421 acacaactta aatgtctttt ttactctatc aagttgacct gaaccataat tataacttat
      481 tattggttgt gctccttgtg taacccccat attaggaagc ataagaattt gcattatact
      541 gctcattatg gccattgaac ctactgctaa atctcctcca tacatttgta atttactatt
      601 taaggctata agtactaaac tttctgtaga ttgcattata aatggagaga ttcccaaacc
      661 aagcactggt aagatgatac ttgcttttgg tatcatatac ttctttttta ttttaagtat
      721 acttttcttt ccaaagagaa acataagtac ccaaatagca gaaacgcctt gtgcaattat
      781 agtagctaat gcagctcctt ttacacccat attaaagcca aatataaaaa tcgggtcaag
      841 tattatgttt attaaagcac ctatagcaac tgtcatcatt ccagttgtag caaacccttg
      901 ggtatttata aaagtattca tacccattga aatctgaaca aacactgtac ctattaaata
      961 aatatttaaa tagtccattg aatatccaat tgtagattca cttgctccaa acatccaaag
     1021 taaaggctct tttccaatct taaaacctat agtcaaaagt attgctaaaa tcaccagtac
     1081 agaaaaactg ttactcatta ttttctctgc ctcatcattg ttttgctcac ccatttttat
     1141 agcagctaat ggacctcctc ccataccgat taaataacta catgcagtta ttataattat
     1201 tattggaaat gccactccaa caccagccat agcaacttct ccattttcca ttctaccaat
     1261 aaaaatccta tcgactatgt tgtataaaac atttacaatt tgtgcgataa tagcaggtgt
     1321 agcaagttta aataataatt tacccacact ttcactacct aagttagtgt ttgaattttc
     1381 cattttaaaa cctcctcaat ccaataatta aaatgtatca ctaaaatttt attctatata
     1441 aaaatttcat tttattagca gacaaattta gagtactttc cctacaagta atactatact
     1501 atatttcttt tgccatagta aacattttag aactaactga agttaaatac tttatctcat
     1561 tttttattta ttattaaaaa tttttcacac atactcataa aaaaactatc caagtatatt
     1621 acttagatag ttttatatta tatgtaaaga aagagctgaa actataaccc ctgataacaa
     1681 attaccaaga aatactccca taaaagcatc ttttattctc atttttaata tggaagctat
     1741 agcagatgca gtccatgttc ctgtagttgg cagtggtata cctacaaata gtattatacc
     1801 aattatcgaa acacttttta atttcttcat tcttgagttt atttttctat caattacatc
     1861 agcaatccct ttaaatagtt tcttccctct taaaaattgt aatacatgtc tgaatgtcaa
     1921 tatcaaggga atcgatacca atgtagaacc tattacacta aatacataaa cccatatagg
     1981 atttaagccc attgcaatac ctataggaat ggctcccctc aattctgtta caggaaccat
     2041 agataaaatt aatatgtgta aatatcccaa actattccct cctattctaa gtgacattaa
     2101 gttatgaatt tatttgctct ataacctcac catccttttt actaactaaa aatgcatttt
     2161 tagtcaatga aagcattggt aaatcactct tagagtctga atatgcatat gaattttcat
     2221 aatcaatctc tatcccaagt ttacctaata cagcctttat tctttttaat ttttcaattc
     2281 ccttacaatt ttctccaatg aattgatttt tatatcttga attttcataa aaaaactccg
     2341 tgcctataac ctcgtctgca agaccataat acttaaaata tttcatatat gcatatggag
     2401 atgcagttat cataattata acattatctt cttctttatt tttgtaaata acctttttca
     2461 tagattcgtt aatttttttc attatgcaat catcaaaaaa cttttttaaa tcttcttcac
     2521 taaaatctac tattggataa aagattgcct cttttgcctt cttcaaaggt atcattctaa
     2581 ttaaatacat aaatagaata aaaataatgt atggcaactt tataatagtt ttaaaggttt
     2641 tattttttaa agagtaaata aaaaaatcta tcatgctatc agtatttata acagtataat
     2701 ctacatcaag taataataca ctttttttca taagtatttc cccatcctat cactaaaaat
     2761 atcactaaaa aatcaatatt attaaacatg taattatcca taagattacg tttattttta
     2821 aagcattatc ttttaggaca atatcagtag gattattttc atttgtagtg ttaattatat
     2881 aattatatct aagtacacca taaactacaa atattgttgt aaatatcata tgtggctttt
     2941 ctggattact tgtagaatac aatgcataac atacaattgt agaacttaat ataactatca
     3001 tcatttgatt tagattttct atgtcataat cttccaatat ttttctatgt tcaactgcat
     3061 catctcttag agtttctatt tcttttttac gttttccaag agctaagtac agagataaga
     3121 aaaatgtaca taaaattatc caatttgata aactaacact tatggctaca catcctgcat
     3181 aaaccctaag tataaaacca agagatattg aaaacacatc taatagtatt atattcttta
     3241 gtttaagaga atataatata ttgtttatca aatagagact gagaattata gaaagattaa
     3301 aactaacaac tgttgataaa cagactgaaa ttaaagctaa tactacaaat agtatattag
     3361 cacttttttt gcttattctt ccacttgcca ctggtctatt acatttttta ggatgttttt
     3421 tatctttatc catgtctata gcatcattca gtgcatatac tgatgatgac attaggcaaa
     3481 aacacacaaa cgcaataaac gtatttaaaa ataaatttat atttagtatg ttgtttgaaa
     3541 aaattatagc tgaaaataca aagaaattct ttatatattg ctttattctc attaacttta
     3601 tataatctga aattttagta agaagcctta tacttgtatt ggtctgtttc ataaacttac
     3661 taccctttca atattatttt ttaaacagga tatgatttta tccctatgtt taaaatttct
     3721 ataaatccat tgacattact gattactata gtaaccaata acagcgatag aaatatatta
     3781 tacatattct tttcattttt tctaagcgct ttcgtaaagt aagccaataa aattataact
     3841 gcaaaactaa aatttatagc cataatactt gaattgacta gattatatcc tactatacca
     3901 tgtaaagtta gattgaatat aattattccc atacatagct taggaactct tttttctgaa
     3961 ttttcaataa ctgataaaac acaaaaacat aaagctaata atgtaagaat tactggtatt
     4021 gtagcaactt ctccattttg caaaaataac ccttttttaa tgcttgctcc aggaaaataa
     4081 aaaaccgaat acaataatct ctctataatc attgcaatct tttccaaaat tggttctcca
     4141 cttataaatt tcgtatcata ctgtattaca tttccaaggt tcataaaact ctcataagac
     4201 ttcgttgaaa ctacacaaat atatgccatt aggataaatg taattaaatg ttttaacatt
     4261 tttcctattt ttaatttaaa ctcaaatatc atattaaaaa aatacaaata tatattacta
     4321 atagttattc caaatagagg aatagctgct aattctacta aataagaatt ttttcctttc
     4381 atcttatttg caataactat gaaaaatatt aatgaaaatt ggctatatat aaatctctct
     4441 acatttaaag tcataaatag ctgtggaaaa gaaaatatca ttatcatagt aagtaaaatt
     4501 aaagttaatc tctgcatttt tatcttttta atatgattaa agactaaagt tattgacata
     4561 gcactagtta ttatttgtag acaaattagt ccaaacccat aggcactatt aaaatctgtt
     4621 gtcccaggat ttgctattga agtacaaaat aggtatattg gatatgcaaa aattgatata
     4681 attgctgaaa acaatatgtg cttactattg tcttgactta tagcaaatac atttaaattg
     4741 aataaaactc ctgtatctgt atctaacaat acatcataag tattgtttaa atcataaaat
     4801 atagtagtct tagaaaaaaa tatcttagtt attattaagt aagttattac aactaatata
     4861 aacaaaaaca tctcgttttt atattttaat attttactca tcaatatatc accatccatt
     4921 cattatatag gatttgctga cacctacaat ttattcctta tatttaatag ccatcttcat
     4981 aatgttctta cttgttttat tgtaattgaa taactcttta tttacaacca caatatttac
     5041 tttattgatg ttataatcag tcaagtattg tttatataac atagattagt gttgtataaa
     5101 atgagaattt gtaagcaata ttaatgtaaa atatttgaaa aactttatat aaaaatatac
     5161 aatgttgctt atggaattaa actataacaa attaaagcat aatttaagga gacaaaatta
     5221 taatctgtct ccttaaatat taaaactata ttttaaaaca ttactaaat
//
LOCUS       DAEOSP010000225         5268 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.229, whole genome shotgun sequence.
ACCESSION   DAEOSP010000225 DAEOSP010000000
VERSION     DAEOSP010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.229"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            27..1517
                     /locus_tag="KRH54_003220"
     CDS             27..1517
                     /locus_tag="KRH54_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG8580851.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            1797..3293
                     /locus_tag="KRH54_003221"
     CDS             1797..3293
                     /locus_tag="KRH54_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBG8580852.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            3438..4364
                     /locus_tag="KRH54_003222"
     CDS             3438..4364
                     /locus_tag="KRH54_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8580853.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKSSTKNTAFKTTILNTGKSDCILVEIGNKVIMIDTGEDKNGKQI
                     VDRLKEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDS
                     LKKADIEPVLLKDNMNIVINGAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGD
                     AEEERLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGV
                     LKILERLNIKTFLTSNGEVVINSDSKTLSVNQ"
     gene            4482..4673
                     /locus_tag="KRH54_003223"
     CDS             4482..4673
                     /locus_tag="KRH54_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580854.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            4896..5246
                     /locus_tag="KRH54_003224"
     CDS             4896..5246
                     /locus_tag="KRH54_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580855.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
BASE COUNT         2081 a          533 c         1018 g         1636 t
ORIGIN      
        1 aaatcattta cgggggtggt attaaaatgt ttcaatttga aacacaaata cacaaattaa
       61 gacatgatgt tttaactgca atagctagac ttggaaaaga agataacctt accagagaag
      121 caatggataa tattcagtat gaaataataa aaggtgagaa acctacatac agatgctgtg
      181 tttataaaga aagagctata atatctgaga gagtaaatgt aagtatgggg ttacaaccag
      241 gtcaagataa gatagattta gatacaatgg atatagaaga agatgaacaa attatatata
      301 ttttggaagc tgcatgtgac agttgtccaa taaatgaatt tacagtaaca gatatttgta
      361 gaggatgtct agcacataga tgtaaagaag cttgtaaatt tggagctata acacatgtcg
      421 gaggaatggc ctatataaat catgaattat gtaaggcttg tggaatgtgt aaaaaagctt
      481 gtcaatatga tgccatctca gaggttgtta gaccatgtaa gagtgtttgt ccaactaatg
      541 ccttaggatt tgatagagaa aatatgaaag ctatgataca tgaggataaa tgtttaaact
      601 gtggagcatg tatgtcagca tgtccatttg gagcaatatc tgataagagt ttgatagctc
      661 ctgtagctag aaagttagtt caaaaagaga aaatgtatgc ggtggtagct ccagctataa
      721 caggtcaagt tgaagcaaat gttacatatg gtcaaataaa aaatgctata aaatcactag
      781 gatttgttga tgtattagaa gcagcttgtg gagcagatgc agttacagtt catgaaagct
      841 tagaatttgc tgaaagaatg gaaaagggag aagcttttat gacaaattct tgttgtccag
      901 gatttgttgg ttatatagaa aaaacatacc caaatcatgt agggaaaata tcttctacag
      961 tatctcctat gatagctagt ggtagattta taaaaagtca tgaaccagat gctaaagttg
     1021 tattcatagg accatgtaca gccaaaaaga gtgaggcagc taagaaagaa ttaaaaggag
     1081 ctgttgatta tgtacttact tttgaagaaa taatggcatt atttgaggca tttgatatag
     1141 acttggctaa gtgttcaaat gaagatatag atgatgcatc aatttttgga agaggttttg
     1201 gagctgctgg tggtcttaca aaagctatag aaaattatct tgctgaaaaa ggtattggtg
     1261 ttaattttga acctgtaaag atttctggtt caagagaaat caagaaaact atgactatgg
     1321 ctaatttagg aaagctagaa ggtaatttta tagaaggaat gatgtgtgaa ggtggttgta
     1381 taaatggttc tggtaaattc ttatcaggtg caaaggttaa agccactttt gatagaaaaa
     1441 attctcaatc aactaagaaa agtgtcttag caaatacaaa gataaaagat tttagtaata
     1501 taaatttaga aaaataaaga taatacaaag caacaaaaat acatattgtt aatagagttg
     1561 ttctaagttg aggtttaact tttggacaac tctattttta tttatatatt taattattga
     1621 acaactaaaa taaaaattat atacacttaa aataaaaatt acatatataa tattttgtat
     1681 ataacttaac aaagtatatg cctatgttat aataatttat agaaataaat aaaaaataca
     1741 atatacataa tagtagaaat atagcaaaaa ggtgtgattt atagggagga ttacttatgt
     1801 ttgaatttga aacgcaacta aaaaggttaa aacatgaagt tttaacagca gtagcaagac
     1861 tatctattga agaaaattta acaaaagaaa atatagaaaa aattccatat gaaataatta
     1921 aaggagaaaa ggcgagatat agatgttgtg tttataaaga gagagaagta gttcttgaaa
     1981 gggcgcaact tgcattaagt ttaactccaa atagtaaata tggagatata aatccagatt
     2041 cagttagttt ggacacagat gagcaaatta tatatatagt tgaagcagca tgtgatagat
     2101 gtccaataaa tgaatttaca gttacagagc tttgtagagg gtgtttggct catagatgta
     2161 aggaagcatg taaatttggg gcaatttctt atataaatgg gagagcatat ataaatcatg
     2221 atttatgtaa ggcttgtgga atgtgcaaaa gttcatgtca atatgatgct atatcagaag
     2281 ttgtaagacc ttgtaaaagc gtttgtccaa caggggcttt ggattttaat agaaacacta
     2341 tgaaagcaat gatacatgaa gataattgta taaattgtgg agcatgtatg tcagcatgtc
     2401 cttttggagc aatatctgat aagagtttga tagcacctgt tgcaaaaaaa cttgcaaaga
     2461 aagaaaatat gtatgcaata gtagctccag ctataacagg tcaaatagat agtaatatta
     2521 catatggtca gttaaaaaat gctataaaat cactgggatt tgtagatatg atagaagctt
     2581 cttgtggagc agatgcagtt acagttcatg aaagcaatga acttattgaa agaataaaat
     2641 caggagataa ttacatgaca aattcatgtt gtccgggatt ttttaagtat atagagaaga
     2701 tgttcccaga tgaaaaagat agaatatcat ctacagtgtc tcctatggta gcaacaggaa
     2761 gattagtaaa aaaaatagat tctgatgcta aagttgtatt tataggtcca tgtacagcta
     2821 aaaagagtga ggcaacagag aaaagtttaa aagattcagt agattatgta cttacttttg
     2881 aagaactaat ggcattattt gaagcattta atatagattt atcaaaatgt tcaaatgaag
     2941 atataaatga tggttcgatt tttggaaggg gatttggagc atctggtgga cttgccaaat
     3001 cagtacagaa ctatattgct gaaaaggggc ttagttttga ttttgaacct ataaaagtat
     3061 ctggtccaag acaaattaaa aagacaatga caatggctaa attgggaaga ttagaaggta
     3121 attttataga aggaatgatg tgtgaagaag gttgtattaa tggagcaggt aagtttttat
     3181 caggtgtaaa agctaaaaat acttttgata gaaaaaatgg acaatctact aaaaagagtg
     3241 ttttatctaa tgataagatt aaagaatttg aagatgtaaa tttagaaaga taattgtttt
     3301 ggtacagtca tttttttatc atgtttttat catgccacaa atatgcctaa aatttgccct
     3361 ttttatatgt gtattttaca gagttaaggt tgctatacta taaatatgta ataaataaaa
     3421 tttataggag cttttggatg aggaaaatag tattaaaaga tatcctgttt ttagttacta
     3481 cattatctat aatagtaata ttattgcttg taacaaatat aactactaat agtaagataa
     3541 aatcaacaag tgaatcttat ttaaaaagtg acaaaaatag tgtgaggcaa aatggtatcc
     3601 agaggagcac aaagagtagc acaaaaaata ctgcatttaa gactactata ctaaacactg
     3661 gaaagtctga ttgcatattg gttgagatag gtaacaaagt cataatgatt gatacaggag
     3721 aagataaaaa tggaaaacaa atagtggata ggttaaaaga aaaaggaata aatacacttg
     3781 actatttaat tttaactcat ttagataagg accatatagg tggagtagat agtgtgttat
     3841 ctagtgttaa agtaaagaat attatacaag caaattataa gaaagatagt aagcaatatg
     3901 atgaatatat tgactcactt aaaaaagctg atatagaacc agtgctacta aaagataata
     3961 tgaatattgt aatcaatggt gcagaaataa acatacatcc ggcttcaaaa agcaagtacg
     4021 agagttcaaa tgattattca ataatcacta atataagcta tggagcacat aagtttttat
     4081 ttgcaggaga tgcagaggaa gaaagattgt cagagtttat aaatggaaat actttaaaat
     4141 atgattttgt taaaatgcct catcatggaa gatatgacaa attaactgaa acatttttag
     4201 agtctatttc accccaatat gctgttatta catgttcaga gaaaaaagaa ccagaggaag
     4261 gtgttttaaa aatattggaa agattgaata taaaaacatt tttgacttct aatggagagg
     4321 tagttatcaa tagtgatagt aagacattga gtgtgaatca ataatttttt taaaaatatc
     4381 taaaaaaatt taaaaaaatc attagcaaat attacccctt aaacatagta tattttagga
     4441 gctgaaactc cacatacaaa atgactagga ggtaacatat tatgttggat agattttttg
     4501 gtgaaggcgg atttggtgga tgtgcttggt ggataataat attattcttc ttattcttag
     4561 cattccaaga ttgttgggct gaaatggact tatgtgcatg gatacctttc ttaatattat
     4621 tattaatact ttgtgaatgt ggtgacgttt ttgatggagg atgtggatgc taaaaaaatc
     4681 aatttagatg atttaataaa gcttatggtg ggtataaata aaataccaaa ccaatcacaa
     4741 tctagtgttt gaaggtagtc ggaaatcccc cgctaccttc ttttttttat tgttaaaatt
     4801 tatttttgaa ttatttcatt tacatataat aatttttacg ataaagtctg atataattaa
     4861 aaagaaaata aaatttattt ataggagatg taaaaatggc tatagtggat aagattttca
     4921 atgagataaa acttatggtt gcaagacttt tattgggaaa aaaatacagt gaatatgaag
     4981 aaaaagattt agtgtataat acagaagaag aagttatact tataacttta aaaagattag
     5041 tttttcaggg aaatgtaaac gaagcagaag aaattttatt tgataaagct aagagtgtaa
     5101 atagtgaaaa tatgcagtat atagcaatag aattttatac tatgcttatg gaaaaaactg
     5161 atgaagaatt agaagctatg aatttttcta aacaagaagt ttatcaaggt atagaagata
     5221 ttagaaaagt attgaattta gaataaaaga tatatgaaaa tattgaaa
//
LOCUS       DAEOSP010000226         5266 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.230, whole genome shotgun sequence.
ACCESSION   DAEOSP010000226 DAEOSP010000000
VERSION     DAEOSP010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.230"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..830)
                     /locus_tag="KRH54_003225"
     CDS             complement(<1..830)
                     /locus_tag="KRH54_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG8580856.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     V"
     gene            complement(1091..3133)
                     /locus_tag="KRH54_003226"
     CDS             complement(1091..3133)
                     /locus_tag="KRH54_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBG8580857.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(3285..4256)
                     /locus_tag="KRH54_003227"
     CDS             complement(3285..4256)
                     /locus_tag="KRH54_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBG8580858.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(4257..5234)
                     /locus_tag="KRH54_003228"
     CDS             complement(4257..5234)
                     /locus_tag="KRH54_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580859.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
BASE COUNT         1707 a          919 c          545 g         2095 t
ORIGIN      
        1 acagatataa ctcttttgat atgtaatgtt aaataaagta gttcatcttg acttacatag
       61 taatcataat tcttttctac atgagttttt attttatatg cacaatcata atccttttca
      121 tactttactt taattatatc tataatttca ttatttgtat cattgatttt ttcttttttt
      181 actaatcgtt ttgcaaagaa ttttaaatga gtcagaagcc tatcataatt tatatcatct
      241 tctttaaatt caactctata ataatatctt ataatattta gtatgtcttt tacaatttta
      301 gtaattaata gtgattcctt tgaacttccc ttatagtttg aatttactat atgcattgct
      361 ataaaaccag cttcatctac tggaaatttc acatcaaatt ctttatttat ataatctaca
      421 gcccatttcc caatctcata ttctttttta tgtattcttt ttatctcatg tagtaaatca
      481 ttctttatag ttatgttttc atgatatctt ttaattgcaa atgcaatatg gtcagtaagt
      541 gctacataaa tatattcata aagcttagta ttcaaattct cctctgcata agcaattatt
      601 tcagtactaa cctcaaaaaa tttctcatca acctggcttg ctagtttact aattttatct
      661 aaaaagcttt catcttgtat tacaaaagtt ctttcaattt tacttttatc tatttcttga
      721 cctttctttt tttgaaaccc aattccacat cctgttaata ttaattcttt ttttatattt
      781 tcatctaaaa ctaatacaac attattatta taaatttgtt gaataatcat aatcaatatc
      841 tctcccaaaa tacaatttcc cttattcata aaaaaatacc taaattaaga tacaattcac
      901 ctgtatacct taacctaggt attgcctgct taccagtaac aatccagctt gttaacgctt
      961 atatattgta ttcaatttat ggttttattc tattataaat aaatatatat gtcaagtaaa
     1021 ttattgaaaa ctatttccta ttttttagta ttaattttaa catcaaaaat caaattttat
     1081 tccattatat ttatgattct atcatcatca tttcatcata gagaatattt tccaataact
     1141 ctttagccac ttctcttgat atttgcttag tttcaactaa ttgttgaaca ctgtctcttt
     1201 gactttgtat cgccattact ctgtagtatt ctactcttgc ctcaaactta tcatcatttt
     1261 gaatctttct agtaatccaa ttcaaataac gattatagaa tctgattgct attaaggcct
     1321 catgtctgtt atcttcatca gtatttttat ttataaatga tatagcagtt ttagtagaaa
     1381 gtgcatatgc atggtgaaac tctttgatta tttctttatt ctcagatact atttttgtaa
     1441 ttttttcttt tacttctttc tttacttttc tatttataat ccacatgctt atttttaatt
     1501 ttacaatttt aaatatagat ttttgaacag attttcttga ttttgtaata taaaatctat
     1561 acaatttaaa ggctctatca gaaattttac catcttctaa tagtttcaaa acagctaata
     1621 atccctgttg gtctgcttct gcaatcatca tacttataag tttattatct ggagtattta
     1681 gtattccttt ttctgagtct accaactgtt catttaaatc atgtataact ggttgtactt
     1741 cttgtgcttt ttcattacta agttttaaaa tggcttcttt caaaattagt ttatatgcct
     1801 ctttatcaga taataaaggt tctgactctt gtacgttatt ttttaataat cttggcagta
     1861 aaactgttgc caatactaaa cttataagaa taacttctga tgatataaat aaaacagtat
     1921 ctctcattgg aaatacatct ttatcagcca aataaaaagg tatagataag gcagttgcta
     1981 gtgtaaatgt accatgaaca ccacaagtag caactattag tgagtattta catttagaaa
     2041 tactctcttt tacaacttct tcatccttaa aacctaaaaa acctacaaaa aaattattta
     2101 aaggattctt tttaggtttt ataaaagaat tatgccataa atatacccat ataaatctta
     2161 taacaaataa aataagtaca attaaagtag aaataaaagt caattccaat gccatatttt
     2221 catttttact actaagtcct gtatatatac ttggtaacaa aaaaccaagc agtgtaaaga
     2281 caaatccatt caatacatac tccaaaacat accaagtatt gtctgatatt atccttaatt
     2341 tagtagttgt attttgtaaa tgctcttttt caattccatg agctatccct gccattacaa
     2401 cagctaggat tccagatact cctatttctt ctgctacaaa atatacaaaa agtggagtca
     2461 ttatttgtat aactattacc attggaattt cttccaaatt ccacttgtgt aaactaagtc
     2521 ttatttttat aattatgtat cctaaaatac ttcctactat tattcctcca aaagctgtaa
     2581 tcataaattg aatccctgcc tcttctattg aaaaaactcc tgtaactgtg gcaagtactg
     2641 caaccttaaa tgctactact ccagcagcat catttaataa agattcacct tcaagtatac
     2701 tcatcatatt tttaggaaaa tttaatcctt gtgtaattga ttttactgct actaaatcag
     2761 ttggagatat tactgctgcc aaagcaaatg aaacagataa tggcatccca ggaataagaa
     2821 aatgtataaa gattccacct gcaaacacgg ttattaaaac taaaccaaat gcaagaagca
     2881 aaattggttt tctgagctct aatagctctt ttctcgatac attttgtcct tcactgaata
     2941 gtaatggagc tatgatacat attacaaata tttctggatt aaatgaaaat gagaatttaa
     3001 atggcaaaat agacaatata atccctagta caatttgata tattgcaaga ggaatattac
     3061 tccacttttt gctaaataca gttgaaatcg ttgtacaaac caataatccc aacaatgtta
     3121 ctaaaattga cataactacc tctcttttca ataataatat tatagattaa acttatatat
     3181 caactaactt aatattaaca gcatgaaatc ccatcttaat caaaaaaatt ttcagtttat
     3241 tctattattc ttattatata catcaaattt aatttttgaa atatctatac tagatgacat
     3301 gcaacaaaat gatttcttga tatttctttt aaaactggaa tctctttttt gcatatttcc
     3361 ttacaataag ggcatcttgt atgaaactta catccacttg gtggatttgt tggagatggt
     3421 atgtctcctt ttaatataat tctatttttc ttaattgtag ggtcttgtat tggtactgcg
     3481 ctcataagcg cttttgtata tggatgttga gaatgtaagt aaagttctcc tttagttgca
     3541 atttctatta tagaacctag atacataaca caaactctat gtgcaatatg atttactaca
     3601 ctcaaatcat gagatataaa taaatatgaa agtttatatt tatcctgtaa atccataagt
     3661 aaatttataa tttgagattg aatagaaaca tctagtgcag aaacaggttc atcacaaact
     3721 ataaattctg gatttaatgc taatgctctt gctataccta ttctctgtct ttgacctcca
     3781 gaaaactcac ctggttttct atttaaatgg tacttagaaa gaccacaact ttctacaaca
     3841 tttaatatat tatcttttat ttcattcttt ggcactaaat tatgttctat aactgcctcg
     3901 gatattattt gttccacatt taatcttgga tttaatgaac tatatgggtc ttgaaatatc
     3961 atttgcattt tgggtcttaa ctttctaagt tcttccttct ctaacttata tatatctaca
     4021 ccattaaatt caactttacc ttcagttttt tcaattaatc taagaatggt tcttcctgca
     4081 gttgattttc cacatcccga ttctccaact atagccaaag tttctccttt ttttaaatca
     4141 aaagaaattc catctactgc ctttacacat ttaatttcct tatcaaataa tccagattta
     4201 acttcaaagt actttttaag accttctact tttaataaac tctcttcttt tttcaattat
     4261 atctcctcct ttttatataa ccaacatgca atactatgac cactattcaa ttctattaaa
     4321 ggaggtatct gagttctaca tttatcacat actttttcac atctatcact aaaataacat
     4381 gaatctggca tcccaattgg atttggtact tgtcctggaa tcgaatacaa tcttttatct
     4441 ttgttttttc caagagcagg ctttgatttt agaagtccaa tagtataagg atgttttgga
     4501 tttttaaata attcaacaac ctctgccact tctataattt taccagcata catcactaat
     4561 actttgtcac acatttctgc aactactccc aaatcatgtg taatcatcat tatagatgtg
     4621 tttaattttt cttttatatt tttcattata tctaaaatct gagcttgaat agtaacatct
     4681 aaagctgttg ttggttcatc agctataagt aattttggat tacatgataa agccatagct
     4741 atcattattc gttgtctcat accacctgat aattcatgtg gatatgaatt atatatttct
     4801 tcagctcttg gtatttctac cagttttatc atatcgatta cctttttctt agcttcttcc
     4861 tttgataaat cttgatgtat cataatacct tcaatgattt gacttccaac agtaaacgct
     4921 gggtttaaag atgtcattgg ttcttgaaaa atcattgata tctcattccc tcttattccc
     4981 ataagttcat cttcattcat ctttaaaatg tctttgcctc gaaagattat ttctccacct
     5041 tctatttttg atgttggaag taacttcatc aaagacatgg ctgttacaga cttaccacat
     5101 cctgattctc caactattcc aatagtttca ccttcattta tattaaaact tacttcattt
     5161 acactcttta caatcccttt atctgtatga aaataaactt ttaaatcttt aacctcaatt
     5221 agtgctttac tcattatttt ctcctctcag caaaattact taagat
//
LOCUS       DAEOSP010000227         5263 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.231, whole genome shotgun sequence.
ACCESSION   DAEOSP010000227 DAEOSP010000000
VERSION     DAEOSP010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.231"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(257..652)
                     /locus_tag="KRH54_003229"
     CDS             complement(257..652)
                     /locus_tag="KRH54_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBG8580860.1"
                     /translation="MKALEFLNKARVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            793..1185
                     /locus_tag="KRH54_003230"
     CDS             793..1185
                     /locus_tag="KRH54_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8580861.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            complement(1285..1701)
                     /locus_tag="KRH54_003231"
     CDS             complement(1285..1701)
                     /locus_tag="KRH54_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBG8580862.1"
                     /translation="MNWQDAFFQDTKPKFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(1716..2627)
                     /locus_tag="KRH54_003232"
     CDS             complement(1716..2627)
                     /locus_tag="KRH54_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG8580863.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            complement(2809..3831)
                     /gene="splB"
                     /locus_tag="KRH54_003233"
     CDS             complement(2809..3831)
                     /gene="splB"
                     /locus_tag="KRH54_003233"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBG8580864.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            complement(4058..5188)
                     /locus_tag="KRH54_003234"
     CDS             complement(4058..5188)
                     /locus_tag="KRH54_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG8580865.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
BASE COUNT         1743 a          903 c          576 g         2041 t
ORIGIN      
        1 gattaaataa aaatcaatta tagttaaaat attttttaaa agaaatctat cccattataa
       61 agtaaatttt aattatctta tttctagtat gtaattattc aaaatagtat atcattattg
      121 aaatacacat ctaataacta aaaattttta catattcatt atttaaaaat ctttttattg
      181 catttaaatt aaatctatca acatttcagt aataacgttt tcttaatttt cactatggat
      241 gtcgattcaa ttataattaa agctctacat caatcttttt accactcatt gaacaacaac
      301 taacttttgc cttatcaata gagaatattt caaacttacc atctcctaca gagtacatct
      361 gacctaaact tggcattatt tcaagtgcct tagcttgtgt ttcttttgat gtaacaaata
      421 ctgcttcacc taaaatacgt agagtattaa ggttttggtc tatacaacaa agttcaacct
      481 ttggattaaa aataagttgc ttaaacatat ccttttgatt agatgtgcaa aatgtaagtt
      541 taccattgta ttccataaca aatcctaaag gacgcacatg tgcttgattg ttctcatcaa
      601 ctgttgctaa ataataaact cgtgccttgt tcaaaaattc taatgctttc ataattctta
      661 cacccctttt aaatacttgt attaaggatt atcctttgat ataattttaa tataaaatac
      721 agaaaaaaca agtatgcagt tttttctgat aaagtataaa aaagatacta ctataactaa
      781 ggagtaatta aaatgagtat aaatactgaa aaaaatataa ctaaaaacgt tgaagaatta
      841 acctgtccaa tccgttatgc attagacatt gtcggtggta agtggaaatt acctattata
      901 tgtatgttag cagttgaaaa tccaattaga tatagtagca taaaaagaaa attggatggc
      961 attacaaata ctatgctagc tcaatcatta aaagatttag agtctactgg tattgttcat
     1021 cgtaaacaat ataatgaaat tcctccaaaa gttgaatata cattaacaag taaaggtaaa
     1081 agtattgtgc ctattttaca acaatttgct aattggggtg ctactaatat gcaagaaaaa
     1141 aacacatgtg gacttagttg caaagaatgt agaaagataa agtaaattta aatttactaa
     1201 attattcata ttatagacta tatattaatt tatctgattg aatataaaaa caggatgact
     1261 tatatattca tcctgttcta tatgttaagc tctaaattta attagttcaa gcatatcgtt
     1321 taataccaat aaactatcta cttgaatcat tagccattta cttccattaa aaaagtttac
     1381 ctcattatat atttttttga tataatcaca acaagtatta ataaataaat caacctctac
     1441 tctattgctt tcacttacaa taaccaaagc tttaaaataa ccttcttgtg gatataaagt
     1501 acatagtgat ttacctcttt ttttatattt tacattccaa cctctttgca ttgaacattt
     1561 actgtattca agcttaggtt ctacattata agtagaaacc aaagttttat ttaacttgtc
     1621 ccataatgga ttattaataa attcattaat ttgctcaaat ttaggtttag tatcttgaaa
     1681 aaacgcatct tgccaattca tattcatcat cctttctatt gttcaatagg aaccggaaca
     1741 atccaatgtt ccagatatgc tgcttcaggt gtagtggggt aatacatctc aattgcaaaa
     1801 tcagtagttg ttaaattatg tttcttcatc catctttcca taaatttatc tgctttaaaa
     1861 acagctgaat taactaactc attaaagttt tctgcctcaa atccacatac taaatattct
     1921 ttagctggca actcaaaagt actatatcct tctactgatg tacctgactc tacctctaca
     1981 cctgccatat acatacaatt tccttctttt gcatttccca tataaagagc agcaaattca
     2041 tttccacctg gaatttgatt tggtatatta tgtctttgac ttccaagttt catccataat
     2101 tcctctacta ttgcaattcc agttgtttca ccgcccacca actggcaaat aggtacttct
     2161 atttctattc caacaaatgt ccttggatta ctcaatgtct tacgattgac ttcaatcact
     2221 atatcatctg caatcagtgg aacaccttca tcaaccatag cataatttaa aattaattct
     2281 ggcttaataa agtgattgag ttttactgga tttgctctat atttttctgg agttaatcca
     2341 tatgcttttt taaaggaacg tgtaaatgtc tcatgactag caaatccaaa atttaaagct
     2401 acatcaagta tacgattctt atgttctgct aagtattcag aagctcttgc aagacgacga
     2461 agctttatat actccattac agtcttattt accaatcttt taaatagtct ttgatagtaa
     2521 aatggtgata aatttgcttt atttgctaat tcattaattg atatttcttc tgataaattt
     2581 gcttctatta aatccaatgt aatttgaata gactcccatg catgcatact ataactcctc
     2641 ctgatatttt tgttactctt atcctttata gtatcaaaac tagaaaaaat gttcttgata
     2701 gatagtgccc tttttaattt tcttatatga tagtgctaaa tctcattgct ttattataaa
     2761 cataaaatgt ataaaaataa aatatagcaa atctttaggt aagatttgct atattatata
     2821 ttttattaaa ctatttttaa atactccatt tatttcctta agaaagaact ctttcatatc
     2881 atctaattgt tctttatcat acacgtactt accatatcca aattgaccgt atttaaactt
     2941 tctatcctca ttttccattg gcaatagtgt atctggataa atctctagta ttctattttt
     3001 tgctttagtt gtatatctat gagatataac ttcaaatata atttttttat caaaatcctt
     3061 tggtaaagct tcttttatac ttataatcag atttttatac tcttctttcc aaccttcata
     3121 taaaaacaca ggtgctatta tgaaaccaat ctgataacca gccctagcaa gttttacagc
     3181 tgactctatt cttttatttg cagaggctgt ataatgctca tgttcattta taattctagg
     3241 agtattgata ctaaatcgta tttctgtatg attattatgt tcagcatcca ataagctatc
     3301 cacatcactg tatttagtaa caaatctaaa tcttcctttt tcctctcttc caaaaaactc
     3361 tattgttttc tttaaagaat tagtatatgg ctctactgga actgggtctg atgtagctgc
     3421 cccttcaaat atggttacct gtggctttct atcttcaata tatttttttg tctgttcaag
     3481 tatttcatct acatttacaa acacccttat aaatggttta tcacctaatt gtgtgttcaa
     3541 ataacaatac tcacatcttc ccatacatcc actgacaagt ggtagttgat aattagcaga
     3601 aggtttacat gtttgaaact tcagactttt tctaatccca actaccatag tctgcttccc
     3661 acttctatat tgttcatata gattttcacc tggaatatgt tctttaatcc tatttgatga
     3721 tatattaatt atctctacat ctttattatc tctaaattta ttgtatattt ttttacccac
     3781 atcagtgtct aaagttcctt tttcgaatat tattcttttg ggattaaaca tttatttcac
     3841 ctctacttaa agtttttaaa tcgaagaatt ttataaaata ttacttaatt aatcttccct
     3901 tttttaaact cctttattta ttagaaagta aaataaaagc ttaagacaaa tttattatat
     3961 atataacttc tatacacaat ttttttacta ttaattactt ataatttttt attattaatt
     4021 tataattttt atataaaact aaatatgtaa agtcaaatta taaagactta tggtacattt
     4081 cctttatttg ttccttatta aaatactttg gatgtctctt aatatttgca actgatattt
     4141 tcatacaatt atcagttaac caatctatat ctgatttttc aatacctaaa tcacttagtt
     4201 ttatatctaa atctattttt ttaaggaaat ttctaattgc atctgaacaa tcttctgcac
     4261 aagttcctcc taataccttt gaaatattac caaatctttc aatatcacta tcccatgaac
     4321 tttctataat agtaggagtc aaagctgcaa gtccttttcc atgcattaca tctctaagac
     4381 cacttacagg atgctccatt ccatgtggca aggctgtacc cgctgtacca atcgtcattc
     4441 ctccaatagt acttgcaagt gttatttttt cccatgcttc taaatcatta acatcattat
     4501 atacctttat taggttttct gatataagtt caatcccata aatagcttgt acatctgcaa
     4561 gagggtttct tccattagaa agatatgctt ccatattatg ggcaagtgca tcaaatccaa
     4621 ctgatgcaat tatatgtttt ggcattgtaa ccataagttc tgggtcaatt attgatgctt
     4681 ttgcatacat actattcttc tttatagatt ttttatcttt agtttctgaa tttgtaagta
     4741 ctgcaaaaca atttccttca ctacctgtac ctgatgttgt tggaactaag attataggta
     4801 aagcttcttc accttgtcta attccaaata tataatcatt taaatctcct gggtttttat
     4861 aagaaaaagc tatactcttg gcagcatcca taatgcttcc accaccaaga cctaaaacac
     4921 aatcacatcc agttgatttt ataatttcta ctcctgaata tatagtagtt ataagaggat
     4981 tttgttctac tctatcaaaa acttcatatt ctattttaga gtcttttagt aaatctattg
     5041 ttttatcaag taatccagtt ttctttgtac tatttttacc tgttactatc aatacttttt
     5101 tgccatattt agatacttct tttcccacat cattaattct gcctctccca aataagatat
     5161 ttactggcaa attataatta aaattcatgc taaaacctcc tatttctact ttagtaataa
     5221 ttttatgttt ttaacaaagt gtaattatgt atttaaacat aaa
//
LOCUS       DAEOSP010000228         5118 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.232, whole genome shotgun sequence.
ACCESSION   DAEOSP010000228 DAEOSP010000000
VERSION     DAEOSP010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.232"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            540..1400
                     /locus_tag="KRH54_003235"
     CDS             540..1400
                     /locus_tag="KRH54_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBG8580866.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGLNLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1390..3261
                     /locus_tag="KRH54_003236"
     CDS             1390..3261
                     /locus_tag="KRH54_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBG8580867.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASMLALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSITLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3281..4735
                     /locus_tag="KRH54_003237"
     CDS             3281..4735
                     /locus_tag="KRH54_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBG8580868.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
BASE COUNT         1980 a          528 c          918 g         1692 t
ORIGIN      
        1 tgtttaaata aaactaaatt taagtattta tatattagta tatcttacta gcatttaaat
       61 gaaactaaat ttgaagcttt acctattaat atatcttaat agtgattaaa taaaactaaa
      121 cttaagactt tatatgttaa tatatcttaa gaatttattt gttaacatac agttcattat
      181 atttaaaagc attactatga taattcataa tagtaaaata gataatggtt cagacaatat
      241 tataaatatt agtgacggta aaatagtatc aatttagtat ataaaccttg tcagataatc
      301 gcataacaga gcttgtagtt atagaataaa aaaatatcta ggaaaccgtt gacagattta
      361 tatggatagt ataacatata gaatatgaaa ttaaacaaaa aagaatttta attatggcga
      421 cgtagtcata attttgagta tgttactgat tagcaggcag aactcaagta tatatgtgaa
      481 aaatagaggg atactctata catatatgct tgagttcttt ttattgaagg gaggaatata
      541 tgaaaataat tcagatttta aataataatg tagccttagt aaaaagaggt aaaaatgaaa
      601 ttattgtatt tgttaaagga attggcttta aaaaaagagt gggacaagta ataaatgaaa
      661 atgaaataga gagaacatat atactagatt catacgatat gctagaacac tttagttatc
      721 tactacaaaa ctcagactcc aataatatag ttcttattaa tgaaataatt gaatatggtg
      781 agaaagcttt aaatataaag tcaagtgatt atctaagctt aaccttacta gaccatttag
      841 agtttttact taaaagatgt gaaaagaatc aatttataaa aagtcctttg gtatggaatg
      901 ttaaaagatt ttatccaaag cattttgaaa ttggaatgca tgcactaaaa ttaattggtg
      961 atagtaaggg acttaatctt cctgatgatg aggcagtatc aatagcactt cattttgtaa
     1021 atatggaggt aaataaagag tctcatgata gtacaattgt agagttgaga actttagcag
     1081 atatagtttc tataataaaa tatcatttta atgtagagct agatgaaact tcaacaaatt
     1141 atatgagatt tactactcat ctacagtact ttatccaaag aattatgtcg gatgaagctt
     1201 caatagatga agataattct atagatttat ataaacaagt aagtcaactt tatccaaagt
     1261 catttggaag tgttcaaaag ataagaaaat atataaaatc acaatttaat gtcgatatat
     1321 ctataagtga agaaacttat ttaatgttgc atataaatag ggtgactcaa agactggagg
     1381 caaaatcaga tgaaatataa agatttaaat gaaaatataa taaaacttat agggggaaaa
     1441 gaaaatataa agtcggtagc gcattgtgtt actcgtttaa gatttacttt aaatgatagg
     1501 agtatcgctc aaacagaaga aattaaaaaa ttaaaaggag taattgatgt agtttctaat
     1561 gatgttgctt atcaaattat aataggtact catgttgtag atgtatataa cgagtttatg
     1621 tctatgatag gtttaagttc aagtgaatca agtataaaaa gtgaaagaaa aaagggaatt
     1681 agaggtatca aaggtatatt taattccatc tttgtggtag tctcagagac tatgacttca
     1741 gtaattgaag tactacttgc agctggtata cttgcaagta tgttagcact tttaaatatg
     1801 tctggcatac ttcagagtga cagtcctaca tatcaaatat tagatacatt aagaagtgct
     1861 acatttcact tcttaccagt attaattgca atagcatcag caaaaagact taatgtaaat
     1921 ccctatatag cagtagtgtt agcagttacg ttattatctt catcaataga tggtgtagaa
     1981 ggattaagtt tatttggtat aaatttacag gctacaactt atgcaaacag ttttattcca
     2041 atacttttag gagtatgggc aatgggaatt ataattgaag gtttgaaaaa aatattacct
     2101 aaagcattac actatttttt agttccagta ttatctttaa taataactct atcaattaca
     2161 ttaactgtat ttggacctat tggtatgtgg attggtgatg gattaaacta tgcatgtatg
     2221 tttttaattg atacattggg taattggagt gttgttgcac tgtatgcagc tttacaacca
     2281 ctcttaatag tctttggtgc agcaaacttt actatgccta tatctttaaa ttctgtgaca
     2341 acattggggt atgacccaat ttttatgggc gctgcaacaa tatctgatat tgcagtttgt
     2401 ggtgcaatgt ttggatattt cttaaaaact aggaataaag aacaaaaaca attatttggt
     2461 acagtaagtt ttagtgcact tatgggagtt acagaacctt cagtgtttgg tgtgtttatg
     2521 aaatatagaa gaccatttgt agcagtaatg ataggtggag gtataggtgg tttaatagca
     2581 ggtttagctc aagttaaaac atatggaatg gtttggggat tagctgcatt accaacatat
     2641 cttgttggag gagaggtatc taactttaca tttatgataa taagtgtagt agtatcattt
     2701 gtagcagcaa cagcagcttc ctatttacta ggaatacctt cagatgaaaa agaagaagtt
     2761 aagggtgata atgaaaaaga attagaacta ataatcaacg agaacaattc aagaactatt
     2821 agtattggaa atgttgcaaa aggtcaggta attgcacttg gggaagttaa tgacaaagca
     2881 ttttcatctg gagcattagg taaaggggtt ggagttatac ctagtgaatc aaaatcaact
     2941 gttatatctc ctgtagatgg aactataact atagtatttc caactaaaca tgcatatggt
     3001 atagtgactg atgaaggatt agaaatatta attcatattg ggatagatac tgtaaactta
     3061 gatggaaaat tctttgaaag tcttgtaaca caaaatcaaa gggttaaaaa aggagatgct
     3121 ttagcagtat ttgaatctga taaaattata gaggaaggct ttgattctac tataataact
     3181 gtagttacaa atactagcga ttatttagat gtaatctcat caaatgatga atcagaagaa
     3241 ttattaactg taatagtata aattttagga gggaaaattc atgaatatta aagatacatt
     3301 tttctgggga ggaagtattg cagcccatca atgtgaaggt tcatgggata gtgataacaa
     3361 aggacctgca attatggatt ttgtaactaa gggctcttat gaaacgccaa gagtaattac
     3421 tgataaaata gaagaaaaat tggattatcc atctcataat ggaatagatt tttataatag
     3481 atataaagaa gatatagctt tatttaaaga aatgggattt agtgcattaa gaatatctat
     3541 agattggtca agaatttttc caaatggaga tgatgaaaat cctaatgaat tagggattaa
     3601 atactatgaa ggtctgatag atacattaat agaaaataat atagaaccta ttgtaacact
     3661 atatcatttt gaattaccta tgaatttagt acataaatat ggttcatgga ataacagaaa
     3721 acttatagat ttgtatttaa agtacagtga gactgtaata agaagatttg atgataaggt
     3781 aaagtactgg gttacattta atgagatgaa ccatattgac cctcaaacag agcactcgga
     3841 tatttttact tatatgatag caggtattaa gtatagtgaa atagaaaata aggttcaaac
     3901 attggcaaat gtagggtata atatgacttt ggcgggtgta aaggttgcaa agttggctag
     3961 agaaatcaat cccaacaata aagttggatg tgtatttgga ctaaatccaa tttatccata
     4021 caattgtaat cctagtaatg tgttaaatgc atttttagat aatgaccgtg attattatca
     4081 aattgatgca atgtgcaatg ggaagtttcc acaatataaa ttaaaagaat atgaaaaaaa
     4141 taatataact attgaagtga ctgatgaaga caaaaaagca tttaaagaag gtaagttaga
     4201 ttttatagga cttaattatt atttttcaag tgtatctact ccaaaagaaa atttatcagg
     4261 tgataaatct ttatttggtg ggattcaaaa tccatatttg gaacaaagta agtgggggtg
     4321 ggcaatagac cctataggtt taagatttac cttgaactat atttacagaa agtatcaatt
     4381 acctatctta attacagaaa atggtttagg ggcatatgat aaaatcgaag aagatggaac
     4441 tataaatgat gattatagaa ttgaatattt aagtaagcat ctatctcaaa tgaagtctgc
     4501 aattgaagag gataatgttg aatgttttgg atatttgatg tggggaccaa ttgatttagt
     4561 cagtgcaact acaggtcaga tgagtaagag atatggattt atctatgttg acttagatga
     4621 tgaaggcaaa ggaagtttta agagatataa gaaaaaatca tttgattggt acaaagaagt
     4681 aataaaatct aatggagaag ttttagattt ttctacggat atagtagtag ggtaggaata
     4741 agataaatag aagttaggaa ctaatgttaa ttagtgtcta gtaaagcatt taaaatagag
     4801 tatagggtct ttaaagtaaa gtgtgtattc ttcttaaaag taatggcaac taaaattact
     4861 ataaaaagga gaatacacat tttttatgtt tatacttaag taaatattta gacatattca
     4921 tagtataatt tactcattat ataaaaatag tatgatttac tcactatata actttataaa
     4981 ttttctggaa aaatgtcaga atatattgac aaagaaaaga tttaaaatta taatctatat
     5041 taagaatagt ttgacaatat aaaaacaagt atttttataa atagtgaaat tatttttatt
     5101 gtaaatttaa gaataatt
//
LOCUS       DAEOSP010000229         5117 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.233, whole genome shotgun sequence.
ACCESSION   DAEOSP010000229 DAEOSP010000000
VERSION     DAEOSP010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.233"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(156..380)
                     /locus_tag="KRH54_003238"
     CDS             complement(156..380)
                     /locus_tag="KRH54_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBG8580869.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(415..1782)
                     /locus_tag="KRH54_003239"
     CDS             complement(415..1782)
                     /locus_tag="KRH54_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBG8580870.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            complement(1866..3401)
                     /locus_tag="KRH54_003240"
     CDS             complement(1866..3401)
                     /locus_tag="KRH54_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580871.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVTYK
                     IDCDVILVNLFELIMNNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            complement(3424..3858)
                     /locus_tag="KRH54_003241"
     CDS             complement(3424..3858)
                     /locus_tag="KRH54_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580872.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGDFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            complement(4119..5030)
                     /locus_tag="KRH54_003242"
     CDS             complement(4119..5030)
                     /locus_tag="KRH54_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBG8580873.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEILEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
BASE COUNT         1607 a          969 c          433 g         2108 t
ORIGIN      
        1 atttttctta tttcttcata tacatatgaa atatttcatt atttctataa tgttttatta
       61 tctttctcat actttttcat atatattgtt tcactatttt ttttacttct ttattaaata
      121 cttatgtata acatacattt aataaacgct aactattaaa cacctaatac ttgagttact
      181 cctggaactt cactcaccaa aacattttca ataatagctt tgattgtcat agtcgctcct
      241 ggacacccac tacatgcccc ttgtagtcta actaaaacca caccattttc atttacatct
      301 ataagctcta catctccacc atctctttgt aatactggtt ttatcttttc ctcaagcact
      361 ttttcaactt tttctctcat agctattccc ccctgaaata ttttattacc aaaattatac
      421 caatggttta aatttttgtc catctaatga tttatcaaat tcaatttttg cttcatcaat
      481 aattttagta tccataaata catcataagc actacaagcc agtactttag ctgaatttat
      541 catagcagaa aatcctatat ttgaacctgc tgatgaacat gactgccatg tatgacttgc
      601 aattccaaca ggccaagctg ccattgcaaa ttgagccact ggtattacat aacttacatc
      661 tccaacatca gtagaaccag ctagtccttt atctttgtca ccgtcaacta tttccttgtg
      721 tatatagctt tctacaatag atttatcatt ttgtaatgat gttgctacac ctaatctttc
      781 ttctctagta agagtatcac acaacttttt tgcaaattct tcttcttcag gagtattttt
      841 agggcaacca acaaaaacca tattgtcata aactaaatca gtcaaacatt ggtttggtat
      901 atagtcatat attccatctg taaccttata ctccatttca gtttctgtca tcattgctgc
      961 tccttgagct acttttatta atctatcaag aacgtgttct gcatcttttg cttttttacc
     1021 tctaataaag taccaacttt cagcaaatcc aggaactaca ttaggtcttc caccaccatt
     1081 agttataaca tagtgcattc ttatagagtc aataacatgt tctctcaaat aattagctcc
     1141 aacattcata atttcaacag catcaagtgc gcttctacca ttatgaggag cttgagctgc
     1201 atgggctgtt atccctttaa atttaaattt tacagataag tttgccagac ttcctcctct
     1261 ccaaggtgca tttatatcaa aaggatgcca tgtaaatgca caatctacat catcaaaaca
     1321 gccatttata cacataactg tctttcctcc accttcttct tcagcaggac aaccaaaata
     1381 ttttattgtt ccattaaatt tattttcttc tattaatttt tttatagaaa caactgctcc
     1441 tacacctgct acacccaaca ggttatgccc acatccatgt ccagcttttc cttctaccaa
     1501 tggctttctt tctgcactta cacattgtga tagttctggt aaagcatcat attctccaag
     1561 tatgccaatc acaggtttac cctctccaaa acttgctata aatccagtag gtaaattttc
     1621 tacttcttca attttaaatc cttttgactt taggtattct ttttgaatgc tacttgcata
     1681 cttttcttca aaggctaatt ctggattttc ccatattttc ttagataaat caattaattc
     1741 ttctcttatt tcttcaacat tatttatgat atactctttc ataattttcc tccaaatata
     1801 tgtatttttg atatgcttta cagattatat tatatactaa cattctattt attcacaatt
     1861 ttttgttaat taattttatt catatgctct tctatggagt atatcttatc ttcactttgc
     1921 ctcttcataa atttaatgta ctctatcttc cataaagtag tttcagtttt ctcatttaac
     1981 ataaattcta atatattaat ttctctcatt ctaatatctt cataaaatac tacactaccc
     2041 aatattgcca attctaattc acttaatgat gcaaacaaca ttaaatactc ttctccaaat
     2101 aaacattcag attcaagaat atcgataaag tcttttttag cattgatatt ttcctttatt
     2161 attcttattt tttcaattat agatgtacta tctaatatct cattgaatat ctctctaaac
     2221 gattcatcat ccaatttact ttcatcatca acaatataat cttctaagtg ttcattttta
     2281 tttttactca cagttaccat acttttaaaa gtgttacttt ttattgagct tagcacagat
     2341 tcaataaata tcttttcata ttttttaata tatcttatta catcttcatc ttctatgctt
     2401 aatcttttta ttaatacttc aattgcttcc ataagtagta aagttatttt tttatttatt
     2461 ctaatttctc tgttaatttc ttcaaaatca ttataatcca aattactatc tacttcagtg
     2521 ttaaaactat catctacttc aatcaattca attatattac tttcttcaga gtctataact
     2581 ttatcttcag taatattatt gtacatattt ttaaattgtt tttctaaaaa ttcatactca
     2641 tattttgaaa tttctaaatt agtcacttca tttcctaaaa tagtagaaaa aatacaatta
     2701 ttcataataa gttcaaataa atttacaagt ataacatcac aatctatttt ataagtcacc
     2761 ccatagtcat aaaataattt ttctctatct tttttcttaa ataggttaca aaatctattt
     2821 tctatatcta tactctcaat ataattcttc acataataaa tacccttaac atccatatca
     2881 tctagtgcca atggataatc tatacttact ggaacgtcct gagcttcaaa ttttacaata
     2941 taacttttaa agaaacttga taactccatc aaggtatcat tatatgctat tgaattagta
     3001 tccaattttc tactcatcat tagtttataa aaatctttta gcctaactaa ctcttcctct
     3061 aatatttcaa ttcctccaag gtacactttt ttaacattct tagcagtcaa ctccacaact
     3121 cttttttcta tatctccaag cttatttata tatgcatcaa tagcatacca tattgaaatc
     3181 aacagttttt cggcaacttc tgtttttact gatgtgcttt gaccatttgt atatttttta
     3241 atcaaatcca taagtattag agatatttct tgttgtatat tatatacttc tctttcatct
     3301 atcactttac aatttagaca ttccttaagt agagatatgg aatattggtt ctttattaaa
     3361 ttattttcgt ttatattaat attacttaaa ttatttaaca tacaaaatct ctcctatcat
     3421 atattaaact tctccaaatt taaatttctt tgcaaattcg tctgcttctc ctcttacatt
     3481 gtccatattg cctgaaaatt tttcataaaa atcacccagt atttctataa tctcatcatc
     3541 acatatcttg tcatcagttt cattttttaa ataataaaaa atttcttgca tatcattaat
     3601 agcctctaaa taattgtcca catttgtata ttgtgatgta tatatatttt ctattaactg
     3661 ttttgtaact tttatatcta attctattct tccatacccc tttaaagtat tatctctcga
     3721 atttattata tcttttacat cagattcaga aagagctaga ccataatctc cagattctat
     3781 attagtttca agcagttcat ttctttctat ttcctttaaa ttctgattta tagaactcaa
     3841 aataataggt gatatcattt ttaaattctc cttttttatt ttttccattg actttttttg
     3901 ttttttatgc tatatttgaa ttagtatata ttattttacc aaccacttat aatagtagtg
     3961 ttaaaagtgg tttttgtcaa ctcaaaatta ctatctcaat taaacttttc aattttctca
     4021 acaaaaaaaa taccatcatt tagatatata attttatata acaactttta attcagtagt
     4081 tatttactaa aatctaaaca atgatatatt tattaatttt atttatcttt tttggctcta
     4141 cttctacttt tctttacatt tgagcaattt ctacaagaag gtgaacagta ttgagattta
     4201 gaatttgtag gaatataaat tttaccacag tacttgcatt tatttaaaga agaaggttca
     4261 cttgtaattg caaaggcata gatagtctca atagctgttt tcaaagaatc aaactcccat
     4321 gcaataattg gtttatctaa ttggcttata gaaaatccta tcttttgagg attaaaccct
     4381 ccagacataa tagtaacact ttcagttaat agattagatg tatttttata tacacaaatt
     4441 tgattaaaat gcttacacat atctttagca aaacttacaa tccaatctag ttgttcagtg
     4501 tagaactttg aaaatactaa atctaaaatc aatggtctct ttccaaaaaa ctttggagat
     4561 tcttctctct ttgatacatc tatacccctt ctatactttt taaaatacac atcttcatca
     4621 cttgcaaatg gtataaacat gtttacatat tcatctgtac tcaatatatt ctcttttgta
     4681 atataattct tttcaatcat cagtactctt tcttctccaa ctatatctct attatataca
     4741 cttgaatata ttaaacccaa taatccatac ttttttacaa attcaagtat ttctttcttt
     4801 agtttcattt cttttgtctc ttttgatata tcttgtaatg cctcttgtcc tatgcgaatt
     4861 atatctacta ttatatcatc tgctacatca aatgggttat acatggtaaa cacagcatct
     4921 tctgttggaa ctatatataa ttcatcttct tcagtaattt tatattcata atcactatac
     4981 ctaatccaat cacttgcaaa attccctatt aattctattt ttttattcat aataatcatt
     5041 cacacctttc aatgtaatat ataatttaat attactatta cacttttagt ttattccata
     5101 ttttctacac tgtcaac
//
LOCUS       DAEOSP010000230         5116 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.234, whole genome shotgun sequence.
ACCESSION   DAEOSP010000230 DAEOSP010000000
VERSION     DAEOSP010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.234"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            269..1423
                     /locus_tag="KRH54_003243"
     CDS             269..1423
                     /locus_tag="KRH54_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBG8580874.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            1474..2727
                     /locus_tag="KRH54_003244"
     CDS             1474..2727
                     /locus_tag="KRH54_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8580875.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            2853..4247
                     /locus_tag="KRH54_003245"
     CDS             2853..4247
                     /locus_tag="KRH54_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBG8580876.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            4262..4492
                     /locus_tag="KRH54_003246"
     CDS             4262..4492
                     /locus_tag="KRH54_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580877.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMSVVIVIFFDYIYRVLKMTRKK"
     gene            4509..5039
                     /locus_tag="KRH54_003247"
     CDS             4509..5039
                     /locus_tag="KRH54_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580878.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNYINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
BASE COUNT         1850 a          539 c         1002 g         1725 t
ORIGIN      
        1 cttaggatga ctttagttat tctaagatat acctattttt gtactgacta ttttaaacat
       61 tgtaaaaaaa atatcaaaaa catattgact ctatagttac tatagagttt ataattagaa
      121 ctgtaaaaaa acaaaataag tgtcatattt ttagaaatta taacaaataa aaaagtatac
      181 ttgaaaaaat gtattaagac taagctaaat tgatgaaatt tggtagggaa aacgttgtat
      241 aaataagtca aaatgggagg aaatcaaaat gagtaacaat attaatcaag atttagagac
      301 aaaaataata cattggggac atagtgcaga cccaacaaca ggtgcattgg ctacaccaat
      361 atgtcaaaca gcaacatttg ctgcaaagac agtagagcat tttgaagagt tgtgtatgac
      421 atggggatat gtgtatacaa gagagtgtaa tcctactctt acagagttag aagctaagtt
      481 ggctatgctt gaaaatgcag aaagtgccat atctagtaca tcagggatgg gggcaataac
      541 atctactata ttagcattgg taaaaagtgg tgaccatata gttagttcag atggaatatt
      601 ttcacacact aaattattca tgtctgagtt gttatctaaa tttggagttg aagttacatt
      661 tgtagatgca gtaaatccac aaaatgtaaa agaagcaatg agaccaaata ctaaaatagt
      721 ttatatagaa agtcctttaa atccatcatt agacttagta gatataaaaa caatagcaga
      781 aattgctcat gaaaataaaa gcttagctat agttgatagt acttttggga caccaattgt
      841 acaaagacca atagatttag gtgcagattt agtaatacat agtcttacta agtttataaa
      901 tggacatggg gatactttag ggggagcagt agcaggttca aaagaattaa tagatttagt
      961 tagatggcca agtttatgtt gttttacagg agcatcttta ccaccaatga gtgcatggat
     1021 gattttaagg ggtatgaaaa cgttagatat gaggatgaaa aagcattgtg aaaatggatt
     1081 agcagtagca gaatttttag aggaagaaga gaatgtggaa ttggtaaagt atccagcatt
     1141 aaaatctcat cctcaatatg agctttgtaa aactcaaatg aatggtttag gtggaggtgt
     1201 agtttcattt aaacttaagg atggtatcaa tggattgaca agagaccaag ctagtagaag
     1261 attaatgaat tctttagagc ttgcaacaat agcaacaagc ttaggggaag agcatacatt
     1321 agttcaaatg aatggggaaa atctaataag aatagctgta ggattagaat cttcaaatga
     1381 tataataaat gattttaaac aagcaataga aaaattaaaa taaaattata tgaatgagat
     1441 tataaacaat aaaattttaa ggagagagaa tcaatggaaa gcaaaaataa tgttaaaaga
     1501 tttttgataa tattcatatt ggcatttgga acaacagcta tgtatagttt accatatatg
     1561 aaatcatcat tttatgaccc aatgcaacaa gctttagcat taagtcatac acaaataggg
     1621 aacttattga gtttatatgg attagtagga atggtatctt attttatagg aggatggttt
     1681 gcagatagat tctcagttag aaaattaata actttttctt taattgcttc aggaatactt
     1741 ggattttatt tctctacatt cccatcatat agtatgatac ttttaatatt tgtattatgg
     1801 ggatttacaa caatattaac tttcttctca gcctctgtta aggttgttag aatgcaagga
     1861 agtgagtcag agcaaggaag aatatttggt ttttatgaag ggttatcagg tgtatcaggt
     1921 acattaattt cttttatagg tctttacttc tttggtaaat ttgctgaaat aacagtagga
     1981 ttcaaatatg tagtatggtt atattctgca gcatctataa tatgtggtat acttctattt
     2041 ttcttagtgg aagagaaaaa agatagtgga gcatcagatg aaggattaag tattaagtca
     2101 ttgttaaaag tagttacaat gccaaaggct tggctaattg gtcttataat attttcaact
     2161 tatttagtat tctcaagttt aacttattta agtccatact tatcagaagt ttatgtaatg
     2221 ccaatgactc tagtttctgc tttatcaata ataagaactt atgttataaa aatgggagca
     2281 tctccagttg ctggtgttat aacagataaa gttggttcat ctataagagt aatgtttgta
     2341 ggatttatat taatgacagt aagtacagct gcttacttgg ttattcctaa gagtacagga
     2401 tttatatgga tagctgtaat aaacatgata atactaagtg taattttatt tggatttaga
     2461 ggtatatact ttgcatctgt ttcagagtct aatatatctt tagaaactac aggagcagta
     2521 gttggatttg cttcatttat aggattttct ccagatgcgt tttattacac tattgcaggt
     2581 aattggttag ataaatatgg acaaacagga tatacatata tatttatttt atctgtagta
     2641 tgtgcagtta tagggatatt tgctacttat gctttaaata aaataaataa aagagaaaat
     2701 aaaggtttaa acaataaaat tgcttaataa caagattact taaatactta taattattat
     2761 ataactctac ctatttttat ggaaatgggt agagttgttc atatttagat attaaagtta
     2821 aaaattaaaa tataagaatg tggagattag atatgttaac attttgggaa aatgggatta
     2881 tagttatatt tgcatttata ttattaggaa taggttatta tttttctaga aatgtaaaag
     2941 atatggaaag ttattaccta ggaaatcgaa gtttaccatg gtccttaata gtaggaactt
     3001 tggttgcatc atggtatgga ggagttggta cagtaggtag tgtagagtat gcggctatat
     3061 atggaatatc tgcatgggca gtttggtcaa tagctgcaca tattgggaga atgcctttag
     3121 cattgtgggt tggtccaagg attcatataa gaaccaatat aacagttcct gatttactgg
     3181 aaagttttta tggcaagcat gttgctataa ttggggcaat tttgatgttt atatattgtg
     3241 cacaacttgg agaaataact gctatgggaa ctataggtaa cgtagcatgg aaaatagata
     3301 aagaattaat aggattaata atggtaggag tagtcgttgt acttacagta cttggtgggc
     3361 ttatgggagt tgccattaca gatatggtat tgtttttttg tatggtattt ggtttaacta
     3421 tggttatgcc aggtcaattt gaacaaatag gagggtttca aggtcttaca gaagcactca
     3481 aagatgcacc acagcttatg caccctacaa agggtatgtc agctatgaaa gcattgatgt
     3541 taattgtata ttcttttgga gcttattcag acccaacttt ttatcaaaga ttttcagcag
     3601 cagattctcc taaggttgga agaagagcct tattaacctg tttttcacta tggatatgtt
     3661 ttgacatggt gttaactatg acaggactta tagtaagagc tacttatcca gaaatggctc
     3721 cagctgctgg atatataact ttagttttag atacattacc acaaggaata agagtaatat
     3781 ttataatagg aattttaggt tctataatat caacattgga tagctattat ctagcagctg
     3841 gggctacatt agcaaatgat atatatggaa gaattacaaa aaaagagtta tctcagaaac
     3901 aattagtaaa gtttactaga ataggtgtat gtttagcagc agttatgggg cttagcgtag
     3961 catttagatt tgaaaatgta tatgatgcat gtatatttgt aggaagtatt tggatgggtt
     4021 cggcatttgt acctatagta ggtgggcttc ttggaaatgg aagaagaaca tctatgggtg
     4081 gtattttagg aatgattgtt ggtggagtga cttttggtta ttttaaaata tttccagttc
     4141 agagctttga gttagagcct cttgtgatag ctataccttt ttcatttatt gcatggatga
     4201 ttggtaatag aataggtgaa aatttaaata cacaaaataa tctataaaag gagaaactgc
     4261 tatgtctaaa ttagtgtcta aggtaaaaaa agatttagaa ataaaatggc ttggaataga
     4321 cggttttcta gtattattgt actttttaat aacaggattt atattttatg gtatattgac
     4381 tagaaatgat attatagttg gaaaaataat tccatatgga gttatgtcag ttgtaattgt
     4441 aatatttttt gattatatat atagagtttt aaaaatgaca agaaaaaaat aagaaagaga
     4501 atgattttat ggatataaga gcgttaccta taagaattac aaaagaagaa gctatagaga
     4561 tagctaaaag tggaaaaaat tctttattta gtaagctaat attaaagaac aaatatgctc
     4621 atgaagttag acttcattat atagagttta aaattatcac acttaaaatt cacaaaacga
     4681 aatccatttt caagaatagg agatattcta attatataaa tgaattaaaa attttagtga
     4741 atggttctac aggttctggt agtatagtac aagattttcc tacatatgaa gttgtaaaaa
     4801 atgtggattg taatattgtg cagtatagtg ataaggacga acgaagagta aaagcaaagg
     4861 caaataaaat ggctatgaga ttaactcata ggtttatggg aggaatccca gagattgaaa
     4921 tagttaaaat tgaaagtatt tttagaccat attgggtagt attttttgga aaggtggagt
     4981 tgaatagaag agttagttat cttccaattg aagctgatgg ttttgttata ggaatgtagt
     5041 atattttata taaatatatt ttacacttat tgtataatac atattgagag aagatattat
     5101 atttagttat ataaga
//
LOCUS       DAEOSP010000231         5111 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.235, whole genome shotgun sequence.
ACCESSION   DAEOSP010000231 DAEOSP010000000
VERSION     DAEOSP010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.235"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            36..1040
                     /locus_tag="KRH54_003248"
     CDS             36..1040
                     /locus_tag="KRH54_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG8580879.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIRDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSV
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            1166..2278
                     /locus_tag="KRH54_003249"
     CDS             1166..2278
                     /locus_tag="KRH54_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG8580880.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQNESKSLPTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            2634..3395
                     /locus_tag="KRH54_003250"
     CDS             2634..3395
                     /locus_tag="KRH54_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580881.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            3398..4216
                     /locus_tag="KRH54_003251"
     CDS             3398..4216
                     /locus_tag="KRH54_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBG8580882.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKTEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            4396..4998
                     /locus_tag="KRH54_003252"
     CDS             4396..4998
                     /locus_tag="KRH54_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBG8580883.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
BASE COUNT         2134 a          484 c          914 g         1579 t
ORIGIN      
        1 taaaaatatc gtaaatgtat gaggagtgaa atttaatgcg tttcaaaaga atattgatat
       61 tgatgttaac tgtagcaatg atagctggaa tgttggttgg gtgtgctaat aacccaagca
      121 ataatcctag ctctagcaaa gataataaaa gtgatataag cgataaaagt aattcaaaaa
      181 aatctgtatc aatgatacta gatattgaag gaacaaataa tgaagctatg aataattcag
      241 ctttattagc attaaacaat gctcaaaaaa aacttaatat agatacaaac aaagtggaat
      301 cagatgattc ctcaactttt tctaatagta tagatatttt atgtaatgac aattacgatt
      361 taattatagc tgtaggagct agatttgcaa aaccattgga gatggtagcc aagaaatatc
      421 caaagcaaca atttgcaatt atagactatg agtatgacaa gcaaccttct aatataactt
      481 caatttctta tgaagataat aaatctggat acctagctgg attaattgct ggaaaaatga
      541 ctgaaagcga taaggttggt tttataggag gaacaaagag ttctagtagg gataaatttg
      601 aatcaggttt tagagagggt gttaaatttt ctaatagtag tataagggat atttcagttg
      661 agtatgcaga tgtttttaaa gatagcaaat cagtagaatc tatagctaaa aaaatgatgg
      721 ataatggagt ggatattatt ttttctacta ctgaagatga ttcaaaagct gtaatagatg
      781 cagtaagggc aaaaaataaa aaagttatag caacgaataa agatcaacat gagttagctc
      841 cagaaaatgt tataagttct gttgtaaaag attttgagaa tcctacttat aatctgatac
      901 aaagctttgt aaaaggaaat tataaaggcg gaaaagttat agaaaacaca gttaagtctg
      961 gaagtactgg gcttgcccaa aatagttctc aaaatattcc tccagatgtg ttagaatatg
     1021 ttaataagaa caataaataa tctactataa tatccaagaa tacttatgac tttagttatg
     1081 aggtgaaatg gatgttataa tagacatata tagaaagaca tatgcagagg taggttagac
     1141 ctatctaaat cagggagtga attttgtgaa aataaaaagg atattgatgg cttgctttgt
     1201 agtagttttt acaattttta tattagctat atgtaatttt gattttggat ttgataaagt
     1261 aaataagaat aattctaata tagatagtgc taaatcgaaa cagaatgagt ctaaaagttt
     1321 gccaacttca gcagaaaagg ataaatcaaa tagcaaaagt aagaataaag aagatgacac
     1381 agaagatgat tcatatgtaa gaaaaactat tggagtattg ttagataccg aaaacttaaa
     1441 taacgaatca tataataaca atgtactatc agccttaaaa agggctgacg aagattttga
     1501 tgttaggata aaacttgaaa aaccaaagac ttctaaaagt tttaaagaga gtatagaaaa
     1561 actgcataag tatgaaccag atttgataat agcagttgga gccagatttg cagagccaat
     1621 taaagaagct gctactaaat atcccaaaca gcaatttgca atagttgact atacttataa
     1681 agttcagcca gctaatgtaa tttctgtatc atttcaagat aatgttactg gatatttagc
     1741 aggacttatt gcaggtaaaa tgactgatac tgggaaagtc ggttttatag gaggagttga
     1801 gggttctagt agagataagt atgaatctgg atttagaaag gggcttaaaa cttcaaataa
     1861 agatgctgag ttactagtta agtattcaaa tatatttgaa aaccctgaat cagctagaac
     1921 tattgcaaag gaaatgagag attctgatag agatgttgta ttttcagcta cagaggatga
     1981 tagcaaaaga gctattcagg tagcaagaga aaatggtggt aaagttatag ctattaataa
     2041 agaccaatac gatatggctc cagataatgt aataagttca attataaaag atgtagaaca
     2101 acctgtatat gaactgataa agaacgttgc aaaagatggt tttaagggaa gtaaagtaat
     2161 ggaatttaaa ataaaagatg gagaacttgg cttgacttca aagagcagta gaaatatacc
     2221 accagatgtt ttggaataca taaaaaaaga gtataacaaa gtgaaagata catattaatt
     2281 attgatgtag aagattataa aaatatactg cttaagttta gattggtgat aaaaggatat
     2341 atttattaaa tttagtctaa ctgtacaaaa tatattgatt gagtttaggt tagcaataaa
     2401 aagatatatt tattaaattt gtgctaactg tacggaatat actgcttaag tttagattag
     2461 tgataaaaag atatatttat taaatttgaa ccaactgtac aaagctatac taattaagct
     2521 tagattaact atataaaatt ataacaataa gtgtgtgggc agaaaaattc tgctacacac
     2581 tttatttata ttcacataaa aaatttatca aaaaaaagaa aaaggagtaa aaaatggaat
     2641 ttacaagaga accatttata ctagatgacg atttgtgtct agctttggtg gacaaaagaa
     2701 ttagttatct aatggaaaat gaattaaaat taagaaatat agagataata aaaacaatag
     2761 aatgtaaaga attatatgaa agcataaaat atcacccgga tatatgtatt tgtaatttag
     2821 gaaaaggaga tataattgtt gcgccgaatg tatataatca atacaaagag ttattaagta
     2881 gatataaatt taatataatt aagggagaag ctaatttatt aaatagatac ccttatgata
     2941 taccttacaa tattgcgata gttggtgact atgctataca caattttaag tatactgaca
     3001 aagcaatatt agattatatt gaaaataata aacttaaaaa gataaatata gagcagggct
     3061 attctaaatg ctcaatctgt atagtagata gaacgtctat aataacatca gataaaggta
     3121 tttggaaaag tatgaagaat acagaaatag agtgtttatt gatagaaaaa ggtcacataa
     3181 atttatttga gatgaattat ggatttatag gtggatgtac agggttgatt tctaaggata
     3241 aactagcttt ttgtggagat gttaaaaaac atcctgacta tgaacgtata aaaagttttg
     3301 tagaaagtaa aaacaaggag atagttacac ttagctgtga aaatttacta gatataggtt
     3361 ctatagttcc tctcatgaca agaaaggagc gataactttg gttgagaagg ataatatttc
     3421 ttccatgaaa tttttgagga ataataatgt aaacgataga ttaatatata cgaatgacat
     3481 tgtagaagta gtgatagatt ctgcaaagca agaagtgtta aataaaaaaa tacttaatgg
     3541 tactaaaact gaacaaagag ctttaaatat tgatgagata tcaacggaaa taactagtat
     3601 gataataagt ataatgaaag agaaactttt gaaagaatac attctaaaat gctatgggcg
     3661 tacatatcct gaagataaaa ataatatata catatattca ctgaacatat ttgctaaaaa
     3721 agagattttt atgagagaaa cagtatttac tagggtgaga aattatatag aagaaaatga
     3781 ttttataaat gtagaaggtt ttattagatt tagaatgaga gaatttatga aatatatttc
     3841 tgctataggt gatattgcag ttgaggagta tttaataaag aaagaccaag atgaatttat
     3901 aagagtttta aaatacttta tagatactca agaagaaaag attgatttgc ttaaagttca
     3961 tataatggaa gataatacat ttgttttata cgataaaaat ggaaataaga tagacagtat
     4021 agatgatgaa gaaattataa atatggtaat aagagaaaat ttaaattatg aggacttttt
     4081 aataagtacc ttattaactc tatgtcccaa aaaaatagaa atattggatt tgttaaataa
     4141 taattgctca aaggaaatta tagatactgt agaagcaatt tttgaaaata aagttagtat
     4201 aattatggaa aactagaatc taagcatata aaagctatcc atataaaatt aaaaaaattc
     4261 tttttaaatc gacacaaaag acttataatt gtatataaag ctatagtata actagtgtta
     4321 ttaagtataa agaatatcta cttgattaat acaatatagc tggcaaatat acaattgata
     4381 aggagtaatt ttataatgga taatttaatt aggatactga tattagctgt tattggtgga
     4441 tttataggtt atgttacgaa tgtagttgct ataagactga tatttagacc aatagagcct
     4501 attaaaatac ctattttaaa tatagaaata gtaggtctta taccaaagag aagagcagaa
     4561 atagcagcaa atgtaggaga aataattcaa gaagaatttc tttctatgga cgaaatactt
     4621 gcaaatatca ttactgacga agataaggaa gaagttgtaa gatacataaa agcaagggta
     4681 aaaattataa tacatgagaa agtttcattt atcccgagtg gtataaaaaa catgatacaa
     4741 gattatctag gagagataat tgagtctgaa gttaagcaaa gtatagatga attgagtaaa
     4801 aatataataa ataaagcaaa tgaacgtata gatatacaaa aaatggtcga agataagatt
     4861 aatgaactag atttatatga attggaagag ataataataa gaatagctaa gaaagaatta
     4921 aaacatatag agtttttggg gcttgtctta gggtttttaa taggtatagc tcaagggtta
     4981 attactatgt ttatttaaat atatgagaag tagcatttat ttaagtacta attatattta
     5041 cctatttgag tgaattactt attaataaca tttagatatt aattgtattt acctatttga
     5101 gtaaattact t
//
LOCUS       DAEOSP010000232         5071 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.236, whole genome shotgun sequence.
ACCESSION   DAEOSP010000232 DAEOSP010000000
VERSION     DAEOSP010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.236"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            89..541
                     /locus_tag="KRH54_003253"
     CDS             89..541
                     /locus_tag="KRH54_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8580884.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            544..852
                     /locus_tag="KRH54_003254"
     CDS             544..852
                     /locus_tag="KRH54_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBG8580885.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            966..2072
                     /locus_tag="KRH54_003255"
     CDS             966..2072
                     /locus_tag="KRH54_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBG8580886.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAV
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            2147..4825
                     /gene="mngB"
                     /locus_tag="KRH54_003256"
     CDS             2147..4825
                     /gene="mngB"
                     /locus_tag="KRH54_003256"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBG8580887.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDKYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIVKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
BASE COUNT         2071 a          608 c          864 g         1528 t
ORIGIN      
        1 agtggaaatt agttatattt aatttaatta ggataatgat tacaatataa atataagtta
       61 agacattatg aatggagtgg aaaaatatat ggtactacaa gaaaaatata tatttataga
      121 taaagactta aaaagcaaag aagaagtatt aaattttatt gcagatagtg cttatatact
      181 aggtattaca actaataaag aggctctttt aaaagacctt ctaaaaagag aaaatgaatt
      241 tccaacagga ataaaagatg gatttgcgat acctcataca aagagtgtaa atgtaaagga
      301 agcttccata ctctatatta aaacaactaa cgagttagag tggaaaactt tagatgatag
      361 taaggttaga tatgtattta gtttgttagc cccacataaa aatgaaggaa atatacattt
      421 gaaaactcta tctaaattag ccacatgttt aatggaggat gattttacaa gtgaagttaa
      481 aaatgctagt gatgcaagtc atttagtaaa atatataact aataaaatga aggaggaata
      541 attatgaaaa tagtagctat aacatcatgt atagcaggat tagcacacac acctatggca
      601 gcaaaagctt tagaacaagc tgcatcaaaa atgggtcatg aaatcaaagt agaacaacaa
      661 ggtgctatgg gacgagtaaa tactataaca aaagaagaag cacaagatgc ggactttata
      721 cttatagcat ctgaccaaac tatatctgga atggaaagat ttgatggtaa aaaagtgata
      781 aaagtcaaaa taggtatagc gttaaaaaaa ccagaagctg tcttaacaaa gtgtatagaa
      841 gctatttctt aataaaaaaa tctcactcta atatagaatg agaaaaatca aaaattaaaa
      901 aattgtctaa tgtaattata acatattatc aaaaaatata acaaacttat ataggaggat
      961 taaaaatgag taaaaaaaat aaatctcttt taagtgaact taagggacac ttaatgacag
     1021 gtatctcata tatgttgcca cttgtaatag gctcatcttt agttgtagct atcccaaagc
     1081 ttacagcttt agcagtgggt ataacaagtt tagatgctta taaaaatgta tctggttttt
     1141 atcatattct ttacttaatg gaacaagttg gttggactgg cataggtcta ttaaatacgg
     1201 tattagcagg atttatagca ttttcaatag gtgagaaacc agctatgggt gcaggtttta
     1261 ttggtgggct catagcaagt aatactaatg caggattctt aggagcagta atagcaggtt
     1321 tctttgctgg gtatgtttgt aagtttttaa agaaaaaaat taaaatatct gggtcagcat
     1381 caggaatgat gccacttata ataatgccac ttataacagt tggattaact ggttttttaa
     1441 tgagtgttgt attagcagga cctttaggaa acataaatac ttcattaaat gcttgggtag
     1501 ctgaaatgtg tcaaaatgga acaaattcgg tggtattggc tttaatttta ggcgcaatga
     1561 taggattcga tttaggtgga ccagtaaata aagcagcttg gatggcagga aatgcattat
     1621 tgttagaggg aatctatcta ccagcgatat tggtaaactg cgcaatagtt atacctccac
     1681 taggatatgg tttagctaca ttcataagaa aatcaaaatt ttccaatgca ttagctgaaa
     1741 caggaaaagg caatatagtt atgggtataa ttggaataac agaaggtgca attcctttca
     1801 ctttaacaaa tccattaaaa ttagttcctg ttaatatgat aggatgcgct ttgggtgtag
     1861 gattatcagc tttgttaggt gtacatgcta taatgcctcc agttggtgga ttatatggat
     1921 ttatatcaat aggttctgga tgggcttact tagtaggtgc aatatttgga gctttagtaa
     1981 tagcaatatt atcaacaact ttagtagact tcaatgaaga tgaaactacg gaaaatacag
     2041 aatttgatga aatagagtta gatataatgt aaattaaaaa cttaatttta gataattgag
     2101 aattgagtgt actctcaatt ctcaattaaa ttttgggagg atttttatga aaaagacaaa
     2161 agtacatgta gttccacaca gtcattggga tagagaatgg tattttacaa catcaaggtc
     2221 aaaaatatat ttaatgaaag attttcaaga tatattagac atattggaag aaaaagaaga
     2281 ctttaaatac tttactatgg atgcacaagc ttctctctta gatgattatt taaaatggag
     2341 acctcaggat aaatatagaa taagagattt agttcaaaga agaagattag ttattggacc
     2401 ttggtatact caaacagacc aattggttat atcaggagaa tctataatta gaaaccttta
     2461 ctatggaata gatagatgca atgaatttgg tgaacctatg agggttggat atgtaccaga
     2521 ttcttttggg caatcagctc aaatgccaca aatatacaga ggttttggaa tagatagttc
     2581 attattttgg cgtggagttt ctgatgatat ggtagataca acagagttta tatggaaagg
     2641 ttctgatggt tcaaaagtat tagcagttca aataccattt ggttattact atggaggtaa
     2701 tataccagaa gatgattcag atttaaaaga atatttaaaa gatataatcg taaaacttaa
     2761 ggttaaagct tctactaata acgtatattt tccaaatgga tttgaccaag caccaataag
     2821 aaaaaacctt cctgatatat taaaaaaagc taatgaaata gataatgaaa atgaatacga
     2881 aataagtgat atagaaaact atatagattc agtaagaaat gaaagaaatg aatttacaga
     2941 gcttaaaggt gaatttttaa atgctaaaca tatgagaata cacaaatcca tattctcctc
     3001 aaggtcagac cttaaaataa tgaataacaa attagaaaat tatattacaa atgtacttga
     3061 accgttatta acattaagtt attcacttgg aaatgaatac cctcacctag ttataaaaga
     3121 tatatggaaa cttatgtttg aaaatgcagc tcatgacagc ataggttctt gtaatagtga
     3181 cacaacaaat gaggatgtat acacaagata caaacaagcc agagatttga gtataaattt
     3241 attagatttg catatgagac ttataagtac tcaaatcaaa aataataatg aagaaataac
     3301 attaacagta ttcaatggat tcccagaagt cagaaatgaa gttgtagaat ttgatactta
     3361 tattccaggt caagattttg tgataaagaa taaaaatgga aaaattctta attatataat
     3421 aaaagaaaaa gaagatatta caaattatat attaactcaa acaataagat taaacccaag
     3481 taaaaaaata tatataccca aaactgtata tagagcaaaa attgcacttt cagtaaaaga
     3541 tataccacca atgggatata ctcaactagt ttttgaattg gatactaaaa ataaatgtga
     3601 aatagaaaca agtaattgca aggaaataga aaacaaatac tacaaaataa cagccaataa
     3661 aaatggttca ttagaaatat tagataaaga atctaataag acttataaaa accaagctgt
     3721 aattgaagac aatggagatg atggagattc atacaactat tctcctccaa gaaaagatat
     3781 ttatgtttca tctttagatt ctgtatcttc agtagaagtg ctaaaatcaa acattcaaga
     3841 agaaatgata cttaaatatt cattgaccat accagcttca ctggaagaaa gggctatagg
     3901 taaagtttct gtgaaaatgc cagtaacaat gaaaatcacc ttagaaccta aagaacaaat
     3961 aattaagttt aatgttaata taaaaaataa ccaagcacta tctcatagag taagagtttt
     4021 atttaataca gaaatagcat ctaagttttc tatagcagat caacaatttg gaataataca
     4081 aagaccaaca gtattagaaa aagaccttgc attgtggaaa agagacaatt actcatggca
     4141 agaaaaacct ataactatcg aaccaatgca aagttttgtt actttagagg atggacaaag
     4201 aggaatagca ttaataactg gttgtgtaag agaatatcaa atagttggtg ataatttaga
     4261 tacaatagca ttaacactat tcaggtcatt tggatatatg ggaagagaaa atttacttta
     4321 tagaccagga agagcttctg gagaaaaaat aatagcaaca ccagatgcac aattacttaa
     4381 agagttaaac tttgactttg gtctttacat atataacaat aaatttgatg aagcaaatgt
     4441 agctaataca gctaagagat tcttaacacc tatacaaata tatgaatatg ctgatttttt
     4501 aaatggtaga cttatatttg cattcaatga cgaaaaacaa atatatgaaa atgaatttag
     4561 tttgatgaat gtaaataatt caaatttcac agtaagtgca ataaagaaag aagaaaaagg
     4621 agatggaatt gtagttagaa tcttcaatgg tatgaaaaat gaggatgcta agggaagtct
     4681 taacatcaat aaaaatgtca atgatgctta tagtgcaatg ctcgacgaaa tctatgataa
     4741 tactagcaag cttaaagtaa atactaaaac tgtagaggta aattctttat cacattgtaa
     4801 agtacaaaca atagtaatta aataatataa aatagcttat acaagatttc aaaataaatt
     4861 gctagtacca tgttaatagt aaagccagat attatgctat ttataagctc tgtaacacta
     4921 aataacattt tgtaaaataa aaaggtgaac cttgtatatt aggattcacc ttttttaaat
     4981 aaatcaacat tctgttttaa aataacattt ataagctgtt ttgaaataat gcttacaaat
     5041 tattttaaaa caatacctat aaatttttat t
//
LOCUS       DAEOSP010000233         5041 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.237, whole genome shotgun sequence.
ACCESSION   DAEOSP010000233 DAEOSP010000000
VERSION     DAEOSP010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.237"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            13..318
                     /locus_tag="KRH54_003257"
     CDS             13..318
                     /locus_tag="KRH54_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580888.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            726..1415
                     /locus_tag="KRH54_003258"
     CDS             726..1415
                     /locus_tag="KRH54_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8580889.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSQDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGCAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            1415..2332
                     /locus_tag="KRH54_003259"
     CDS             1415..2332
                     /locus_tag="KRH54_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBG8580890.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKCINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            2405..3331
                     /locus_tag="KRH54_003260"
     CDS             2405..3331
                     /locus_tag="KRH54_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580891.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFSKKVEETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            3328..4008
                     /locus_tag="KRH54_003261"
     CDS             3328..4008
                     /locus_tag="KRH54_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8580892.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIIFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            4106..4441
                     /locus_tag="KRH54_003262"
                     /pseudo
     CDS             4106..4441
                     /locus_tag="KRH54_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
BASE COUNT         1915 a          531 c          802 g         1793 t
ORIGIN      
        1 ggaggggtca aaatgcctag catgattatg gaagttgtag gagtactatt tatagctatt
       61 ggattaatct tgacattcat tgaaataaaa ggaaatttcc cttcttttta tggttttggt
      121 caaaattcta tagggaaaaa tgctgtaggt attatagcag caggaattgc tttaattttc
      181 tttagaaatg gcatagatag catagattct atagctactt atggtgtatt tttagcaata
      241 gaacttgttg taatagttgc atatttctta ataagaaaaa aatttactaa agaagataaa
      301 catttaagaa ctaattaact aaattatcta ctataaaatt ggaaaattaa aaacaaataa
      361 aagtttgttt ttaattattt aaaatccaaa tttgattatt tttaaagaaa ctatgttctg
      421 tataaaacta gaatgtggtt tcttttttat gtataaaata ttttttagca aaagttaaaa
      481 ggtgttttat aatataaaat agttaattgt agatgttaat aaaagaaaat tatgtatatt
      541 tcagataaag ataagagagt atactatatt ttaattcttt tttcaataaa gcttattgaa
      601 aagaacttta acttagaaga gtatcaccaa attgcagata caatatcata agcagttatt
      661 tattgacaac cctttttaat aaatatatca tttgaagtat actgacgaaa aggagttgaa
      721 taataatgga catcttattg gttgaagatg ataagaatat tcgagaaatg gtatacgatt
      781 ttctgattag tgagaattac tctgttaaaa ctgccattga tggaaatacg gctattgaaa
      841 tatttaaaaa ttattcattt gatattattt tactggactt gatgttacca aagtctagtg
      901 gttttgaagt attaaaatat atacgaagtg tgagtgttgt tcctgttatt attactactg
      961 cgaaagatag tgacacagat aaaatgctag ggcttaatct tggtgcagat gattatatta
     1021 caaagccttt ttcaatcgta gaattgcttg caagaattaa agctaatatt agacgtgcaa
     1081 cagtgtacag cagttcaaga aactctcaag atatggttat tcagtataaa gatttgatta
     1141 taaacatatc aaaatatgta gtttcaaaga atggaattga cttaaactta acacatacag
     1201 aatttgaaat tgttaaatta ctagcgacta atcaaggatg tgctttttca aaggaacaat
     1261 tatacaacgc tatttggaaa gaaccttatt atggaaatga aaatgtgcta aatactcata
     1321 ttaacaggtt aagaaataaa ttgtctgatg gtatagaaaa aaataattat attaaaactc
     1381 tttggggaat tggctacaaa atggaggaag aataatggag ttatttttag ttgtaatttc
     1441 gattgtacta tttgctctct acttaaaaga acgaaataaa tcaaagaaaa tggaaagtga
     1501 ttataagtgt atcaattcaa gacttcgtga cataacaaat gatgatgaaa ttaattatat
     1561 tttagtacca tctgatattt cgattgtaaa agaaacagca gtagatataa ataaacttct
     1621 tgaaaagttt tacagcaggc aaattaatta taatagaagt gaaaaagcga tattgcaaat
     1681 atttacaaac atatcacacg atttacgaac tcccatcaca gtattaaagg gttatattga
     1741 aatgctatat cttcaaagtc aaaaagaaga attatcttct tcaatgagag caactgtgga
     1801 aaaaatgcaa acaaattcaa atgagcttgt taattcaata aataacctgt ttaatatggc
     1861 aaaaatccaa tctggagata tgatattaaa tcttcaaaaa tccaatctaa cacaattatg
     1921 tcatgagatt attttagagt tttatgattt gctagataaa gaaaacttta atgttgaagt
     1981 taatattgaa gatagaccta tatatgcaaa tgttgacaca gaagcattac aacgtatact
     2041 caaaaattta attgataatg ctataaaata tggttcagat ggtagatttt tagggatttc
     2101 actatatgaa aaggaaaaac atgtttttat agatattgag gataaaggtt caggtatttc
     2161 tgaaactgaa aaagagcata ttttcacaag agcttatact gctgacagaa aaaaaggcaa
     2221 tggtttaggt ttggctattt ctcaagggct tgcaaactct atgggaggtt caatttatgt
     2281 aaatagtaga cctaatgtaa aaacagtttt tacggtaaaa ttgaaaagtt agaaaaaagt
     2341 tagatttaaa agagagaaaa gacaatttta ttttgtatac tgtttttaga aaggagggag
     2401 tatgatgaac tatatcatag agacccatca attaaagaaa atatataaag ataaagctgt
     2461 tgttaatgct gttaatattc atgtgaaaaa gggagaaatt tatggatttg ttggaccaaa
     2521 cggggcaggt aaaagtacag taatgaaaat gttgctaaat cttgttaagc caaattctgg
     2581 agaaatagtt atgttcagta agaaagtgga agaaactgat tttgaaatat taaaaaagat
     2641 tggtactatt attgaaaatc cgtatttata tgagaatttg actgctaaac agaatcttga
     2701 tttacactgt gaatatatgg gttattataa caaagaacat atatcagagg tattggaatg
     2761 tgtcggattg tcaaagcaat caaacaagaa agtttcaaaa tattctttag ggatgaaaca
     2821 acgtcttgca atagctagag caattcttac taaaccagaa ttattgattc ttgatgaacc
     2881 tataaatgca ctcgacccag aggggataag agaaatgcga gaattattcc gtaagttaaa
     2941 tacagattat ggaataacta tatttatttc tagtcatatt ctttcagagg tagaacaaat
     3001 tgcggacact attggaatta ttcaagatgg taaacttatt aaagaaattt ctatgtcaga
     3061 tatacacaaa tatcaaacag attatgttga agtagatgta gataatgtgg agcttgctgg
     3121 atacttgtta gaaaaagaat ttggaataaa agactttaaa attacttcgg aaagttgtat
     3181 tgaaatatat gatttacgaa aagatgtaaa agaaatatcc aaaatattta ttcaaaatca
     3241 aattggtata aatagcattg gacgtaaaca aagttcttta gaagattact tcttccaaac
     3301 tacaggtaca ggaggtaaag aaacactatg atacatttaa tgaaactaga gctaaaaaaa
     3361 gttgggctaa aaagatattt tttattttca atgctgggga ttttcttatg tatgtttttt
     3421 gtctttactg gattgaatga taaatctact tcaatatatg attatgatgt tacatttagg
     3481 acaatcagtt tatttttctg tttttactat attatattat ttgctgtgtt gaatgtggca
     3541 tatataataa atgaatatac aaataaaaca atcttgattt tattctctta tccacttgac
     3601 cgtaaaaaac ttattttatc aaagttactt ttgattacga ctcttatgat atcatcaatg
     3661 atagttggat atatctgttg ttttacattc gttgttgtgt tggataagta ttttaattta
     3721 gttattggag attttcatat ttcaatttta tcttattgga taccaggagc aattaaaagt
     3781 attattatat tttgctcatt aggtatatgg acattcgttg ttggaatgat aagaaaatca
     3841 atacctgtta cccttgtcag ttcaattatt tttatcaatt taagacaatt tattttagct
     3901 ggtacaaata caactcaaga cagtttgatt tctgttttta ttatagttgg tataactgtg
     3961 attggtatat attatatact aaatcataaa gttactgaaa ttgactagat actgtggcta
     4021 ggaaggtagt tattaattgt atcttcttgg gataataatg atttgctatt atatcagcta
     4081 ttattaatat tattagagtt ttaataatat tgcacaacca tttgaagttg agccaaagct
     4141 tgatgaatta attgaatggt actatagtca gagtgaagta tctattaaag ttatagctga
     4201 atttcattat agatttgaat tgatacatcc atttcaagat ggtaatggtc acataggtag
     4261 atttgttatg ctaaaacaga tgttagaaaa taatcttcct ataaaaattg tatcttggga
     4321 tagtgaggac ttgtatagaa actccttaaa ttcttgtagt ttaggtaatt atgtcccttt
     4381 aattgaatat ttatcaagtt tagaagattt tagagaagta tataaaatgt tatggaaata
     4441 attgtttgca gcaaaaaaaa attttatata aatatatttt tatctatgct atttaacgct
     4501 aatttcttag ttattcttag cttggactat atccactatt ttaactatat aaaatagtgg
     4561 atatagttta actatctctt tataactatt cttatgtatc ttagttgttt cttaatatgg
     4621 ggacagttca taaaactaaa agtgattttt aattttatca tttataattt accattacat
     4681 cagaaaaatg cttatcatta gaattttcat aaaagtcacc tatttttttc aattaactaa
     4741 atcaattcta ttagtcttaa tctttcgtat ttcaaattga taatataaga aaataaaaaa
     4801 taaaaaattt taaatttttt atttattgcg aaaaaaataa aaaggtgcta tactttttgt
     4861 aacatattat tcaaattatt aagagatagt aaacatttat agtttaattt gtagtacact
     4921 agcatgattt gcagaacaat gaattaatct atagtataat agtttaattt aaagtacaac
     4981 gatttaatct atagtacact agcatgattt gaagtacaac gatttaatct atagtacaat
     5041 a
//
LOCUS       DAEOSP010000234         4955 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.238, whole genome shotgun sequence.
ACCESSION   DAEOSP010000234 DAEOSP010000000
VERSION     DAEOSP010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.238"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            98..2599
                     /locus_tag="KRH54_003263"
     CDS             98..2599
                     /locus_tag="KRH54_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBG8580893.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVKNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            2759..3427
                     /locus_tag="KRH54_003264"
     CDS             2759..3427
                     /locus_tag="KRH54_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8580894.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            3411..4688
                     /locus_tag="KRH54_003265"
     CDS             3411..4688
                     /locus_tag="KRH54_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBG8580895.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
BASE COUNT         2066 a          526 c          882 g         1481 t
ORIGIN      
        1 atatagattt ctaaataagt aatttatcaa tattacatat caaaataaaa cgttacaaat
       61 tagctgaaaa tatattgaaa gaaaggaaga ttttattatg agttcaaata aagtggtaga
      121 aagttataaa attacaggta tgacatgtgc agcttgtgca aaggcagtag agagagtaac
      181 taagaagatg gatggagtat atgaccaaag tgtaaatata gctactgaaa aacttaaaat
      241 agaatatgat aattctaagg ttaattttga tgatataaag caggttgtag aaaaagcagg
      301 atatggaatt ataaaggaag aaagtaataa aaaaattgat atgaaaattg atggaatgac
      361 atgtgcagct tgtgcaaaag ctgtagagag agtggtaaaa aaattggatg gagtagaaag
      421 tattagtgtg aatattgcaa cagataaagc taatatagac tatgacccat caaaagtaaa
      481 attatctcaa ataaaagcag ctattgaaaa agctggatat aaaccaatag aagaggtaaa
      541 aaataaggtt gatgtggatg aagataaatt gagaaaagaa agagaaatga agtctttatt
      601 tgttaaattt atagttgcaa tagtatttgc agtacctctg ttttatatag caatgggacc
      661 tatgataata aagcctatag ggccatggcc tttgccagaa attataaatc ctatgacaaa
      721 tacttttaat tatgcattaa ttcagttaat tttagtaata ccagtaatga tagctggtta
      781 taaattttat ataaatgggt ttaaatcact tttttcttta agccctaata tggattcttt
      841 agttgcaata ggaacattag ccgcattttt atatagtcta tatacgacac tacaaatagc
      901 caatggacaa attcaaggta tgcaccatca tcagttgtat tatgaaagtg caggaataat
      961 aatagcactt atcttacttg gtaaatattt agaatcaaaa tcaaaaggaa aaacatcaga
     1021 agcaattaaa aagcttatgg ggctacaacc taaaactgca atagtgttag ttgatggtaa
     1081 agaagttgaa actcctatag aagaagtaga gataggagat atactattag taaaaccagg
     1141 aactaaaata cctgtggatg gagttgtaat agaaggatat acatcagttg atgaatcaat
     1201 gcttacaggt gaaagtatac cagtagaaaa gaatgttgga agtaaggtga caggagctag
     1261 tattaataaa aatggtgtta ttaagtttaa ggctgaaaaa attggtggag atactgctct
     1321 tgcccaaata ataaagttag ttgaagatgc acaaggtaca aaagcaccta ttgctaaact
     1381 tgccgatact gtatctggat attttgtacc aatagtaatt gcaatagctg tagttgcatc
     1441 attattgtgg tttttaatag gaggaaaaga tatagtattt gtacttacta tatttatatc
     1501 agtacttgta attgcttgtc cttgtgcatt aggattagca acaccaactg ctataatggt
     1561 tgggactgga aagggcgcag aaaatggaat cttgataaaa ggtggagagg cattagaatc
     1621 tgcacataaa gtaaatacag tgatatttga taaaacaggt actataacag aaggaaagcc
     1681 aaaagtaaca gatatagtat taaataataa tgtaaaagaa gaatatctta tcaagattgc
     1741 ttcaagtgca gaaaaaggtt cagaacatcc tcttggagaa gcaatagtta agtatggaga
     1801 agagaagaat ataaaatttg aaaaagttga taattttaaa gccataccag gtgctggtat
     1861 acaagttact attaatgatg aaagtatatt acttggaaat agaaagctta tgaatgacaa
     1921 taatataaag cttggggatt tagaagaaaa atcaaatata ttggcaagcc aaggtaaaac
     1981 acctatgtat atagctgtag atggaaattt atcaggtata atagcagttg cagatgtagt
     2041 caaagaaagt agtaaaaaag caatagaaat acttcatgat atgggaataa aagttgcaat
     2101 ggtaactgga gataatgcga aaactgcaaa tgctatagca aatcaagtcg gtatagatat
     2161 ggttttggca gaggttttac cagaagataa gtcaaaagaa gttgaaaaac tacaaaatca
     2221 aggaaagttt gttgcaatgg taggggatgg aataaatgat gccccagctc ttgcaaaagc
     2281 agatataggg atagcaatag gaagtggaac ggatgtagcc attgaatctg cggatatagt
     2341 acttatgaag agtgatttaa ttgatgttcc aacagcaatt aaacttagtc atgaaactat
     2401 aaaaaatata aaacaaaacc tattttgggc atttggatat aatacaattg gtatacctgt
     2461 agcagcagga atattatatg tatttggagg acctttatta aacccaatga ttgcagcagc
     2521 agcaatgagc ttaagttcag tttcagtcgt atcaaatgca cttagactta aaaacttcaa
     2581 ggcatataag agagattaga ttaataaatt aaataaaatg tatccaaaag agctatcttt
     2641 aaattaaaag gattaattta caaattaaga tagctctttt ttcatgtaaa attcacaaca
     2701 ataagataaa atataagtaa tatacgaaat tttaaataaa atagaaagga ttgatagaat
     2761 gaattcgtca atacttgtaa ttgaagatga ttcaaatata caagagttaa tatcagaatt
     2821 tttaagtgca gaaggttatc aagttgatac agcaaatgat gggttggaag gtatacaaaa
     2881 gtttaagcaa ggaagttatg atttagtaat attagatata atgatgccaa acttagatgg
     2941 ttatggagtt tgtaaaatga taagaaagtc atcaagcgtt ccaattatat ttttaactgc
     3001 tttaaatgat gaaggagacc aacttaaagg gtttgactta gaatgtgatg attatataac
     3061 aaaaccattt tcatttaatc ttcttataaa aagagtagaa gcaattttaa ggcgaagtaa
     3121 caagactata aatgacaaat ttatagtttt tgaaaaatta aaattagatt taaacacata
     3181 tattgcagag atagatggag aacctataga acttacacta aaagagttta atatattaaa
     3241 agctttgata gaaaagtatc cacaagttat aactagggaa ggtcttttgg atagtatatg
     3301 gggttatgac tattatggag atactaggat tgttgatgca catataaaaa atataagaaa
     3361 aaaaatatca ttaccatata ttaaaactgt aaaaggaata ggatatacac ttgagaagga
     3421 tatttgataa gtgggaaaag ttaagtataa agtataagtt attttcaata acgacatcat
     3481 tattaatagc attagcttta ataatatatc taattttata ttttttattg ccatcatatt
     3541 atcatgagta taaaattgag tctcttcaag aaagtttgaa atctttagta gatagttcaa
     3601 ttcatttcga tacatatact ttagaagaaa gactttatta tatggctaaa gaccaaaatt
     3661 tggctatttt attaaaagat aaccaaggta aaattgtata tggtaagaat gaagtagtta
     3721 ttttaagata tagtaagtac atgattaata gtcttgagga tgagtataga acttctatac
     3781 ctatatatac taaagatgca aaagatggtc catatacttt agaactcgtt atgccattac
     3841 aaccaattga cgaagcaaac gaagttataa gaaaactaat gccatacatc atatcaatag
     3901 caatattgat agcgattatt ggagcatata tatattcaat tgtaataaca aagcctctta
     3961 taaacataat agagagtgag agagaacagg aatatagaag gaaggatttt gttgctacaa
     4021 tatcacatga actaaaaact ccaataacca taataagtgg tcaaattgaa ggtatgattt
     4081 atagtgtagg taagtataag gatagggata cttatctgaa aaaatcgtat gagtgtacac
     4141 aagaattaaa agatttagta aatgaaatga tagaggtttc aaaatcagaa atacttgaaa
     4201 aagatttgaa attggtatca ataaatataa gtgaattatt aaatagactt gtaaagagac
     4261 aagtattttt aatagaagaa aaacatatga aaactatttt aaaaatagaa gaaaatttgg
     4321 aaattaaagc agaccaagag agaataacta aggcaatcaa taatataata aataatgcca
     4381 taaaatattc tcctgaagaa tcagagctta taattagact ctatgataaa aataaacgaa
     4441 taagtaaaaa gacttctaat caaagagtgg ttttagaaat tgaaaatact ggtgtaacta
     4501 tcgagaaacg ttatttagaa gaaatattta atccatttta tagaatagaa aaatctcgtt
     4561 cgagaaaaac tggaggaagt ggacttggac tttatatagt cagccaaatt tttaaaagtc
     4621 atgggtttga ttatagcata aaaaacaaag aaaactctgt agtattcaca gtagaattta
     4681 aaaattaatt acttattata aagaatatat aactttcaat aatagttcat tttaaaatca
     4741 caaaaaattc ataatgaaac tgtattatat aaatataaat aagcttaaaa ccataaaata
     4801 caccaactat aatgatgaga tttccttaca tcctccatat aagtcatcat tatagtaacc
     4861 caacaattaa gttactatga atttaaaaaa taacaccgag catataatct atgcttagtg
     4921 ttatttttat tttgaaataa aaaatattta attta
//
LOCUS       DAEOSP010000235         4838 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.239, whole genome shotgun sequence.
ACCESSION   DAEOSP010000235 DAEOSP010000000
VERSION     DAEOSP010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.239"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(63..827)
                     /locus_tag="KRH54_003266"
     CDS             complement(63..827)
                     /locus_tag="KRH54_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBG8580896.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
     gene            1101..1775
                     /gene="fsa"
                     /locus_tag="KRH54_003267"
     CDS             1101..1775
                     /gene="fsa"
                     /locus_tag="KRH54_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBG8580897.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GIKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(1897..3291)
                     /locus_tag="KRH54_003268"
     CDS             complement(1897..3291)
                     /locus_tag="KRH54_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBG8580898.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(3369..4715)
                     /locus_tag="KRH54_003269"
     CDS             complement(3369..4715)
                     /locus_tag="KRH54_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8580899.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
BASE COUNT         1886 a          676 c          623 g         1653 t
ORIGIN      
        1 aagaatattt atcaaataaa aatttatatt tataagaaat cctataaaac atataattaa
       61 acttataaaa catttacatt aaattctgat aaaatatcta ctatatcagg agtaggcgtt
      121 ttttctgtaa ttatagaact aatatcttct aaatgagcaa acttgaaatt tccatcaaat
      181 ttaaactttt tatcttccat aactataaat acttttttac ttgaatttat tatagccttc
      241 ttcgtaagac catcttcaat ttcaaaagtt gtaagtgaag agttgaatac atctactcca
      301 caacttccca taaaagtctt atcaaactta tatttcgata taaactctat agttggagta
      361 ccgacaaaac cgtctaaact gagatttaat aaacctccag tggaaattat atttaaatta
      421 tttggttttg aagtaactac atttagtata tccagcatat ttgtaacaag tgttactttt
      481 ttattacttt cagctaacaa ctttgcaagc aagatgttaa ttgtagagat atctaaaaat
      541 atagtttctc tatcttcaat taaattaaat gctttttctg ctattatttt ctttgttgga
      601 atattaattt cttttctgtc ttttgtatcc tgattctcta gtgtttctct agctagtaca
      661 gctcctccat aaattcttct tattgaagaa atcttttcta tattttttaa atcttttctt
      721 atacaatctt ctgtaacatt aaatttagaa ctgagctctt tgacagtaac tctaccttct
      781 ttttttagga tatttaatat agcttggatt ctttcttctg caaacatttt ctacctcttt
      841 tcgcaatata gtattatagt aaatattata agataaaata caatatatca actattttaa
      901 catgaaatac aaaattaatt taatataaaa agtatgtttt ttctaactat atatttagaa
      961 tttagtaaat aaaaaaatta taaaactaag aaggaaaagt aaaacaataa taaaaaataa
     1021 aaaacaataa gcaaaatgtt gaaaataata ccaaatcaat atataataag aataaagaaa
     1081 gattaaaggg ggatatttta atgcaactaa tcttagatac aggtaatgtg gaggagatta
     1141 aggagttatg tacttgttta ccaatagatg gtgtaactac aaatccaagt attgtttcaa
     1201 aagaaaaaaa gaattttaag caacttataa atgaaattgg tgagattata ggtgaggata
     1261 tgcctataca tgctcaagtg ttaagtacaa agtatgaaga aatattagaa gaagcattat
     1321 atataagtag tttgagaaaa aatatatatg taaaaatacc tgtaacacaa gatggtttaa
     1381 gagctataaa agaccttcac agaaaaggta taaaaataac tgctactgca atattcactg
     1441 ctcatcaagg ttttttagct gcaaaggctg gagcaaatta tattgcacct tatgtaaata
     1501 ggcttgataa tatatctggt gatggagtag ctatggtttc agaacttata aaaataatag
     1561 atacttataa aatggataca aaagtacttg cagccagttt taaaaatgca caacaagtta
     1621 tagaattaat gcaacatggg gtgcatagtg ttacagtacc atatgatatt tgtaaatcta
     1681 tgatgaatca tccattaact gattggagtg tagataagtt tatagaggat tgggagaata
     1741 cttttggaaa aggaagtaaa acaaataata tttagtatta tggtaaaaga gtagttttgt
     1801 ccaatttcac aagaacttat ataaaaagat gaaaatatga attttctttt taataatttc
     1861 gttattatct taagttaatt catattttca tcttttttag atttctagtc ctataaattt
     1921 ccaccaaggg aatgttatac atattattat aaagtaggtt aaaggaatca aagttaaacc
     1981 gtatttgaaa ataaccttgc tattatacag attttcacca tgaccaacca ttattgtagc
     2041 atgatgaaat ggtaatatgt actgtatgct tactataatg tagacaaata gacataaagt
     2101 aacactattt tcctttagta tatgtaaact tccaagtaac ggtattacta cagatgatgt
     2161 agtgacagca cttccaagac atatattcaa gattattgtg agagttatca aaaacaatat
     2221 tgaaataatt tctgaagagt ttggtataaa ctttatcaaa taatttccca ttatatctac
     2281 aactccagag tactttaaca ctcctccaat tgaaaatgct gctataaaaa aaataaggac
     2341 cttccaattt atttctttta aagtactaaa tccaactatt tttcttagta ataaaagaag
     2401 tacacaaatt gacattataa ttaatgtgtt tatgttatgt atgaactgag ttaaaaacat
     2461 aaaaaacatt attcctaggc ataaaaatac gtgtttttgc tctttgttta gcaaattaaa
     2521 ttcttttttt aaattagaat atgaaaaatg aagtttatta ccctcaagat tttcattaga
     2581 aataaatttt attgaatgca attctttttt aaagacaaag ataaataaaa aataagttat
     2641 aaaacaagta attatagttg gaacagacat ataaaaagcc cattgacccc aatttatatt
     2701 acaatttcca agttctagta caacataatt tagaagagtg tctccattta caaaaaacat
     2761 tgatgtaaag gtagatgctg taaatatact aaataaaagt atagattttg aatcttcagt
     2821 tatctgttgt tcttttaaaa actccttgta aaaagcagat acaagaataa cccttggaaa
     2881 tggttgtggg ataaaaaata ttaaaagtat acctaaaata tacgataaaa gaatcatttt
     2941 tttaggggta tttacagtat ttaaaattaa cttctcagaa ataatttttg cgacacctgt
     3001 ttttgtaact gcattagtaa ttatgtatgc aagaattatt atgtaaaaat tttctgtaaa
     3061 taaaaactgt aaaataacct ttattggtgc aatgttaaat ataaacgcta gaattacaaa
     3121 aaacaatgca acaaaagttt tttcaactga gtctacagcc catatcaaga tagttaaaaa
     3181 agataagcat atagaaaatt ttatgttgaa actgtaatta agagagaagt agagaattat
     3241 ggtagctata acagtcatat aaaattgttt tggcttaaga ataataccca tttttatatt
     3301 cacctccata atttattata tcataatgga ggtgaataca atgaatgtga atacatttta
     3361 taaaaagatt attcattgat tctatgattt acaatagctt tagttttcca atgtcctttc
     3421 ttatagtaga taactgtaac aataagacct aaagtccaac caaaaggaat acccatccaa
     3481 attccgtctg ttccaaaaaa tttagaaagc atataagaag ctggaagtct tccaagccat
     3541 aaagatacta ttgagcttat aagtgcaaac atggaatcac cagcaccttt cattgcactt
     3601 gaaagtacaa aagaagttcc aataaataag aagaatggtc ctattataaa taagtaagaa
     3661 gagccaattt taatgacatc tttatcagta ttgaatagag caattagatt ttccctaaat
     3721 agcaaaatta aagcaataac tgtaactgct aaaactaagg tcatttttat agattctcga
     3781 atgccttttt taactctttc attttctcca gcacctatgt tttgagcaac aaaagtagaa
     3841 acagcagaac caagattcat aataggtaag cttataaaat tttctatcct aagaccagca
     3901 ccaaaagctg ccatagcaga tgtaccataa ctattaacta aaaattgaag agccatattt
     3961 ccaattgaaa acaacatttg ttgaactcca cttggaagtc ctaatctaag tccatcttta
     4021 aatattttgt tatcatatac aattcccttg attttaaatt taagcacttc atgtttttta
     4081 tttaaataaa ttatacttat tataaatgat actgcctgag ccattatagt ggcaagtgca
     4141 acgccctcaa cacccattct tagcactaca ataaaagtta agtctaaaac tatatttaga
     4201 attgtggcta ttatcagaaa ataaagtgga gttactgaat ctccaagtcc tctaagtatt
     4261 gcacttactg aattatatcc agcagaaaat aatattccac taaatattat agtaagatac
     4321 aattttgatt gagcaaaaac ggattctggt gtattcataa gcttaagcat tgggccacca
     4381 aatactattc caagaatact tattaatata gaaccaataa ataaaagtat atatgtagta
     4441 tcaatagtag cttttacctt ttttaaatct cctgagccat aaaattgtga cactaaaata
     4501 gaaaatccaa ggctaacacc cataagcgct gccaccaata aaaacattat agggttagct
     4561 ccagagactg ccgccattgc ttcttttcca ataaatctac caactattat actgtcagct
     4621 gtattgtata gttgttgaaa taaactacca ataagcatag gcatggcaaa aaagaatatg
     4681 gactttccct catgacctgt agttaaatct ttcacattat tcctccaaat aaaaaaatta
     4741 ttaatttatt atagtataat cttaacataa actttatatc caaactataa ataaattgta
     4801 tcaaaatatc atatattttt ttaaaatgta aagaatat
//
LOCUS       DAEOSP010000236         4821 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.240, whole genome shotgun sequence.
ACCESSION   DAEOSP010000236 DAEOSP010000000
VERSION     DAEOSP010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.240"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            270..3368
                     /locus_tag="KRH54_003270"
     CDS             270..3368
                     /locus_tag="KRH54_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG8580900.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFNDNHLLKSLASLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            3491..4777
                     /locus_tag="KRH54_003271"
     CDS             3491..4777
                     /locus_tag="KRH54_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG8580901.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
BASE COUNT         1945 a          442 c          860 g         1574 t
ORIGIN      
        1 atacttaatt gaggtggaga tttgtcattt atatgagaat taattataaa cacataatga
       61 gataattaat tataaatata ttaaattagg tattacaaaa ctctattgta cgattgacac
      121 aataataatt ctatttacta aaaaatagaa cctcattaag gtactatttt aaaacataaa
      181 gaagtataat atcattgcca aatatatagc aaaattttaa aagctttaaa aattcaatct
      241 ggaaaatgaa taaaaggaga tatatataaa tggatgtaat aaatttaata gatgagttgg
      301 atgaaattgt atatgtttct tctttagata catatgaact tttatatatt aatgaaattg
      361 gtaaaaaggc aatgggactt aatgatatat cccatttaaa atgttataaa gcattacagg
      421 ggaaagattc tccctgcgaa ttttgtacaa actctatttt aaaagaagat gaatattaca
      481 tatgggaaaa tacgaatact cgaacaaata gacattttat attaaaggac aagttaatta
      541 aatgggaggg caaacatgca cgattagaaa tagctcttga tattacagaa aaagaagata
      601 taagtaagtc aatagcagaa aaattagaga taaaggagtt attagttgga tgtattaaac
      661 atcttataac agaatctaat ttttctgatg ctgtaaatct tgtattaacc aatattggta
      721 aatttcataa agctgataga gcatatgttt ttgaagtatc agaagataga aaaaaagtaa
      781 ctaatacata tgaatggtgc aatgaaggtg ttgatgaaca aaaacaggca ctaacaaatc
      841 ttgatattaa tgaattaaag aattggtttt tattattcga acaaaaaggt tttgtaataa
      901 ttgatgatat agaaaatata aaagaagaac agccaataga atatgaaatt ttaaaacctc
      961 aaaaaattga aagtttaatg acatcttcac taaaacaaga tgataaagta gtagggttta
     1021 ttggagttga taatccacat aaagctgttg gagatacctc tcttttgaca tccctatctt
     1081 attttttatt aactgagatg aaaaaaagaa aagtagaaaa taaattaaat tatatgagct
     1141 actatgactt cttgactggt gcatataata gaaataaata tattaagtat atagataatt
     1201 ttcaaaaagg aaaagttaaa tccataggag ttgtatttgt agatataaat gggttaaaag
     1261 atattaatga taactttgga catgctaatg gcgactctgc tattgtgtca gcatgtgtaa
     1321 taataaaaaa atactttcat tcttataatg tttaccgtat tggaggagat gagtttgttg
     1381 tattatgtga agatatatca aaacaactat ttgaagaatt agtgagttgg ttaagtgaag
     1441 aatttaagga tgagatgata tatagtgtag cagtgggcag tatttggagt gacgataata
     1501 ttgatataca taagctaatt aaagttgctg atgagagaat gtaccaagat aagaagaggt
     1561 attataataa gaacaaactt agaaataatg aaatgaaagt aattagtgac ttagctgatt
     1621 cgacgaataa tattttattt aaacataaag agttagttga taaggtttat tttgatgatt
     1681 taatgataaa agggtattct aaggcttatg aaaaattagc ttataataca tatgatataa
     1741 ttttatctat ttgcattgat acgaatttaa ttactccaat ttatcaatca gagagcaata
     1801 tttttagttt tcctataaag gaaaatcttg aaaaaacttt ttcatacata aaaaaacatt
     1861 taattcatcc tgatgatata atattattta atgataacca ccttctaaaa tctcttgcta
     1921 gtcttaataa tagcaagcaa gacgatttag aactaaattt tgagtataga cgtttaggaa
     1981 tagataaaaa gtactattgg gcattaattt atatgttaaa gattagtagt aaaaataagt
     2041 taggtgagaa acaaacacat attttagttg ctataaaaaa tatagattat cttatgcaac
     2101 tacataaaaa ccaaaaagat acattaacag gactatacaa ttatgaaagt ttttgtgaac
     2161 aaataagttt aatgcttaaa agatatcctg acaagcgtta tgcaattata atgatggata
     2221 tagataagtt caaggtaatt aatgacattt atggagttag aggaggagat aaagctttaa
     2281 tatatgttgc agagttgatt caaaaaaaca ttggttctaa tggtatatgt tgtcgtatgt
     2341 atgcagatgt attttgcatt ttttatgaga gttactctga tagagatatt cataaggtaa
     2401 tttataagat taaaaatagc ttaaatagaa aaaaatttga atatatgtta gtaccatgtt
     2461 ttggagttta tagaattttg gataatcata ttccaattac aaatgcgtgt gaaatggcag
     2521 gatatgctca caaaaaagta aagaaacaaa gtatcaacaa ctatatattt tatgatgata
     2581 ctataagaaa tgatgcactt gaagaaaaac agatggaaag ggaaatggaa tacgcacttg
     2641 aaaatgggca attccatgta tatttacaac ctaagtataa tcttaatgca aataaaattg
     2701 ttggtgcaga agcattggtt agatgggttc atccagaaaa aggtataata cctccaatta
     2761 aatttatttc actatttgag aagaatgggt ttattatcag attagatatg tatgtatggg
     2821 aacaagtatg ttctttgatt agaaaatgga tagatttagg tgaagagcca gttcctatct
     2881 ctgtaaatgt atcaagatta catttgcata atccacattt taaagaaatt gttttaagtt
     2941 taataaataa atatagtata ccaaaatctt ttttagaatt agagcttaca gaaagtttat
     3001 ttattaaaaa tataaaatta tttactaaga taatagccga ccttagaatg gatggtattg
     3061 ttttaaatat ggatgacttt ggttcagcat attcttcatt aaatatgtta aaaaatatta
     3121 atattgatac attaaagata gattgtggtt tctttaatga ggaaggaatc acagacagag
     3181 aaaaaatagt tttaaagcac actatagcca tggcaaaaga tttagatatg gatgtagtgg
     3241 cagaaggtgt agagacaaaa gaacaagctg aatttttagt gagtttagat tgtgtggtaa
     3301 ttcaaggtta ttattattgt aagcctattc caagtgctga ttttgagaag ttactttatc
     3361 aaaaataagc taattattcc attctaaatg taggtaggat ggattagttc aaaataatta
     3421 aatagaagta gaaaatagtg aggatataat ttttaaaaag tataatattt atggattagg
     3481 agcgaagttt atgatagtgg gtattatagg tccttcagac tctgggttta agataaagga
     3541 tgatttaaaa cagatagaca gtgatttaaa gactaaaata tatgtaagag aaaaagtagt
     3601 agatacaata gaagttattt ctaaatgtga agacgaatgt gatgctatta tatttacagg
     3661 atgtgcagtt tatgaattta taaaatctaa gtatgagata agtaagccac attcatttgt
     3721 accgagaagt gggacgagta taatgaaggc attttgggca ataaaaagtg caaatataaa
     3781 attagataaa ttcagtatag atgtagttga tagatatgtt gtggaagatg cacttaaaga
     3841 atttgatatt aaagcaacga gtgtattttg taatccattt tctttggaag taggagagtc
     3901 tgaacttgta gaatggcata taaaattatt tgaagaaaat aagacggata taatgttaac
     3961 tggttttggt gcagtatata atgaattaaa agaaagaggc tatcctgtat ttcgtttaca
     4021 agctactata caattaatca aagaaagtta tgacaaggta aagagtgaat atgctctaag
     4081 taaggcaaga ttttctcaaa ttgctgttga aatattgagt ttaatagatt ataaagagaa
     4141 gatagataac tattattctg acatgataaa aaaatcagat atggataagc ttgtagtaaa
     4201 ctatgtaaga agcattcaag gttccttatt tttacttgga agaaatgatt atgttgtatt
     4261 tgttcataaa ggggtagctg ataatgagta taattatgat aagctattca atctaaagag
     4321 agagataaaa gacatgggct tttctcttag tataggaatt ggtactggag taactgcgta
     4381 tcaggctgaa aataatgcac ataaagcatt aagacactcc atagattcaa aagaaattgg
     4441 tatatatttg gtagatgaag atgaaaacat aagaggacct ttagcttccg aaaatgaatt
     4501 aaattattct ttgatgctat ctgatgaaaa ggtaatcgaa atatctaaaa agactggaat
     4561 gagttgtgag tcagttgcaa aaattattgc tataaatgag aatagaaaaa gtaagattta
     4621 tgattcaaaa gagttagcag agtatctaaa tgtaagtgaa agaagtgcaa gacgtatatt
     4681 aaataaaata gtaaatgcag gtttaggtcg aatatgtgca aaagagacta gtattggtgg
     4741 aggaagacca aaaaatatta ctgaaatact gttttaagat attttataaa actgtataaa
     4801 tcattatatt gtataaaatt c
//
LOCUS       DAEOSP010000237         4796 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.241, whole genome shotgun sequence.
ACCESSION   DAEOSP010000237 DAEOSP010000000
VERSION     DAEOSP010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.241"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            391..621
                     /locus_tag="KRH54_003272"
     CDS             391..621
                     /locus_tag="KRH54_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG8580902.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            614..1195
                     /locus_tag="KRH54_003273"
     CDS             614..1195
                     /locus_tag="KRH54_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG8580903.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(1773..2450)
                     /locus_tag="KRH54_003274"
     CDS             complement(1773..2450)
                     /locus_tag="KRH54_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG8580904.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSNTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(2639..4003)
                     /locus_tag="KRH54_003275"
     CDS             complement(2639..4003)
                     /locus_tag="KRH54_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8580905.1"
                     /translation="MKESDKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTVFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKECG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            4131..4652
                     /locus_tag="KRH54_003276"
     CDS             4131..4652
                     /locus_tag="KRH54_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBG8580906.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         1808 a          634 c          759 g         1595 t
ORIGIN      
        1 ttaacctgtg gttaatagag tagggtgtaa ctaaagataa acttcaatat gagtttaact
       61 tgtggctaat agaataagat tgtgaataaa gttatagttt tagatgggta ttaacctgtt
      121 actaatagaa tatgattagg tgtaaaatta tagagctgta taagaaaata ttatagatat
      181 attaaatatt agataataaa tatttatata gatacacaaa agaaaaggcg atatgaagct
      241 aaacttaatg acttcatatc gttttttatt tgaaactcct ttgtatttag agtgtgattt
      301 ttaatattga ggttttatgt gattaagtca cagataaaaa gtaagtatct tgatataatt
      361 cttatagatt aataatatag ggaggttatt atgtctaata aaaaaaagaa gtatgcaaat
      421 attttagcaa atgagtgcgt tgcttgtggt agctgtataa aagcatgtcc aagaagtgct
      481 atctcagttc catgtggcat tagtgcaaaa attaatagag atttatgtgt tggatgtggg
      541 atttgtgaaa agatatgtcc agcatctgta attgagatta taacaatatt aaaagaggaa
      601 gaaggtaagt gtcatgaata ataaaaaaca ttggtatgac tatttatgga ttttttcttc
      661 aatatatttg atattgggat ttgtaaatat tattttcgct tggattgggc taatttgttt
      721 ctttgttcca ctagctattt ctattgttaa gggaaataaa gcttactgta ataagtattg
      781 tggaagaggg caactgttta atttgcttgg aaataaattg aagctgtcaa gaaatagaga
      841 tattcctaaa tttattagaa gtaagtggtt tagatatggg tttttgacat tttttatggt
      901 aatgtttgct aatatgcttt tttcaactta tttagtgttt gaaggaagta gaaattttaa
      961 agaagtagtg actttgttgt ggactataaa aatgccttgg caatgggcgt atcatggaac
     1021 tttagtttca ccttgggtgg tacagtttgc atttggtttt tatagtgtta tgcttacatc
     1081 aactgttctt ggtttgatta ctatgatatt gtttaaacct cgttcatggt gtgtttattg
     1141 ccctatgggt actatgactc agatgatatg taaggcaaag agtaagtctg tatagaaagg
     1201 ccttatgtga tatttacagt tttgttcaag tagagatgag tagtaggttt gaatatatat
     1261 gacaaatata tgagtatagg ggcatagatt tagtttgtgt aaggcaaact gatttatgct
     1321 tcttttttat gtcttagatt attggctaag tagaaacaat gaatttagat attaagaatt
     1381 attcagttat ttgaagaaaa tagaataata attaacatta tatgaatgga tattcattac
     1441 atataagcag ttaatagcaa gatttattta atatgaaata ggtcaagtat ttaaactaaa
     1501 taaggtcatg acaattaata taagtataga tataaaaaga ttagaagtta taacttataa
     1561 aaggtattct atataagtag tatgtggaaa atagtcaaat taacttatta gaaacaaaaa
     1621 atatacataa aaaataaatg taaatatcaa ataagatact atataaaaat agatataagt
     1681 atctttttta ttattgaaat tatgaaaata tattttattt tacacaaaaa agagtgcctc
     1741 atgaactaaa aagttcattt tgagacacac ttttacagtg tcaaagaacg aagtttatcc
     1801 ttatccaata cttttatacc accacgagat aaagacacaa taccttctct agcaaaatat
     1861 ttcaacattc tcgatacaac ttctctggca ctacccatat acttagcaat ttgttcatga
     1921 gtcatattta tagtgttact gccattttta gctatctcat caattaaaaa agatgccaaa
     1981 cgcttatcaa aactcataaa aagaatttgt tgcatagccc acataacatc agaaaaacgt
     2041 tcggtagcta atttatatga aaataactct gcatgaatat tttctgtaga taattttgca
     2101 aaaacagagg aactaatctg tataatatcg cagtccgttt cagcatctac atagacatca
     2161 aaagttattg tttttagaat acaggaagct gataaaatac atacatctcc atcatcaagt
     2221 cggaacagtg taacctctcg accttcatct gaaagtatgt atgtacgcac tgttccagat
     2281 tttataatca aaataccaat acagtcacta tcaccacaat gtactggatt cccttttttg
     2341 taatgcgagg tatttgctga agaaattaat aattcttttt gtaagtcagt aagtttatcc
     2401 caaaaaggca agctatgtgt aagtatatca atttcatttt gtgaaatcat acaatatctc
     2461 ctttcttaaa ttgttataat cccatgatac aagtgaatag ataaattgac aagtagtttg
     2521 ttaagaggat tatttagtca gatagtaata gtatgattta tgtagaaaaa atatgataat
     2581 atatattctt gctattaatg agaaagtaat atgttttgat gatagtaggt ttgtataact
     2641 aatgcatacc gtttattttt aaaagattaa gttccttatg taaccttata gacataaaac
     2701 cagcaattat agcagccatc aaatcagcta ttggttgtgc atataagata ccatttaacc
     2761 catagaatac aggaataatc aaaattatag ggataaaaca gacaccttgt ctacacatac
     2821 ctaaaaatcc acattccttt gcttttccta gtgcgaggaa taatgtagaa tacactgtat
     2881 agaatccaaa tagcataaat gaaaacccat ttgccctaag agctttacta ccattttcta
     2941 ttaataaaat gtcaccactt gtaaattgtg aaattataga ttttgggaag agtatcaata
     3001 taagaccaag cactacacaa aatactgtag tccataatat ggaagttttt attgctatat
     3061 gtaatctatc gtagttcttt gaaccaaaat tataaccagc aattggttgg aaacctttta
     3121 taaaaccaaa tacaacaaga ctacctaaag atgttattcg tgttacagca cccattccag
     3181 ctatcacaga atcaccatac tttgtagcct ccatattagt taatgtaatg gcaagactag
     3241 tcaataattg aaatattaag gtaggtatgc caatttttag gatttctgaa aaaatttctt
     3301 ttgaaaaaca gcattcgtta attctaaaac taaatatact tttcttgctt aaaatatatc
     3361 ctacataaac aagtgttgaa gcaatctgtg agatagctgt tgcaatagaa gcacccctta
     3421 cccccagacc aaaagtataa atgaatatcg ggtctaaaat catattaagc aaagcaccta
     3481 ataacatagc acacatagtt gttttagcag ctccttcact tgtgacaata ttagtcatag
     3541 tgacattgaa tatattaaaa atagatgaca cgacataaat gctagcataa gatattgcgt
     3601 aaggcataat actttttgta gctcctaaca attttaaaat tggattgaga aaaatcataa
     3661 tcacaataat aactaatgta cccactataa tactactgta aatagatgta cttaccactt
     3721 ttcctgcttt ttcattatca ccatggccta aaagtctggc aatatatgaa gcagctccat
     3781 ttccaaacaa taatcctaat cccacaacaa gctgacctaa tggaaatgca acggatatag
     3841 ctcccatttg acttgttcca agtccaccca caaagtatgc atcaaccaaa ttgtataatg
     3901 cagttatcat catacctatc atggtaggta atcccataga taacaatgct tttgagattg
     3961 gggcagagcc taataattca attcttttgt cactttcttt catacttaac actcctcttc
     4021 tattgacatt aagtctaaat tataatacta taaattatag taagtatgga aaatagtact
     4081 ataatttata gtatttgtca atagtaagaa aactaaagga gatgattttt atggctacaa
     4141 tagatttaat tgtattggga atagtaaagc aacaacccaa gagtgcttat gatattcaaa
     4201 aggcagtgga atacaggaat atatcacgtt gggttaaaat aagtacacca tcaatttata
     4261 aaaaagtaat tcaattagaa caaaagggat atattgaaag taaaactgtg aagaatggaa
     4321 aaatgccaga aaaagcagtt tattcattaa ctgaattagg tgagacacat tttttagatt
     4381 taatgtttga tacatctagc aaaccaataa atattctttt agattttaat gctgttatag
     4441 taaatttaaa tagccttaca ccagaaaaac agaaattatg tctcaataat attgaagaga
     4501 atgttcaaga gttaaagaaa actattgagg aaaatataag tataaagaca gaagtaccag
     4561 agacagcaaa agcagtattg gaacaacagt tggtgctagc acaagcattg gaaacctgga
     4621 taaattcttt aaaagataat atgggaaatt agaattagag aagctaactt aataatcatt
     4681 aaacaaaaac cagcatattt aataatgaga tattagaaaa aataaaacaa atgatataaa
     4741 ctgaaaagta tatttaaaat aaagctcact taattgaggg agaatgttaa actttt
//
LOCUS       DAEOSP010000238         4790 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.242, whole genome shotgun sequence.
ACCESSION   DAEOSP010000238 DAEOSP010000000
VERSION     DAEOSP010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.242"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            865..1527
                     /locus_tag="KRH54_003277"
     CDS             865..1527
                     /locus_tag="KRH54_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8580907.1"
                     /translation="MSILEIKNLNKIYGKSETEVKALSQINLKINSGEFVVIVGKSGS
                     GKSTLLHIMAGLETPTNGDVIIDNINISSLDEKKRSALRKEKLGLIFQSYNLIPVLTV
                     EENIKLPTINIKNKDEAYINELMELLGIKDRRMYLPNQLSGGQQQRVAIARALVNKPS
                     IVFADEPTGNLDTKTESDVLSLLKESQKKYNQTIIMITHNIDITKYADRVIEIEDGFI
                     RE"
     gene            1536..4043
                     /locus_tag="KRH54_003278"
     CDS             1536..4043
                     /locus_tag="KRH54_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8580908.1"
                     /translation="MKLNYIVKKYIMGNKKNTLLIVVSIVISTALFLIMNIISEDATN
                     LMINQAKNEFTLKHAQYINPKDEEIKYIENNTSIDKVGKSMLLGISDIGKGQTLQILS
                     QDKVAEELNDIYTLEKGKLPVAENEIAIDSWYIKQKKIKNPIGKRITLDYRRQGIDQE
                     DLYTGEKEFKITGILNSNPILKAQGTSIGLISNDCAIKNIPTKNKYDQVMFTFKKEKN
                     IQRQSQKLIKNGNLNENNIYFNNELLIAISDSMNLKIPYIIVNIVLALATILLIYNIF
                     YILVSNRIKDFGIFRALGFNPNDIFKIMILEVSIYSIISISIGLILGGIIASLSREYV
                     IGVIYNVNYINSIKNQNYINTYIISILLSLGTIIISVSKPLMLSVKTDPMICIRRNNE
                     KVDIKQNSFINKFMIKLFKDYGNIASKNIQRNKKRTNLSIASMVIIFFLMATIYTKST
                     SNFLSDGVLKYWIQGDYLLHNIDISTIRSNNKGYDKNVLQEIKAIDGVTKVNSYRHKW
                     FNIKIDDKSINKNSDYWKKNKDNIEARSEVKDGIKVYNNSFEFLGIEDTDILEDFLIN
                     GKENIDKFSKQPYLYITKKSSDSLNIKKGDRIKVYFDIIDSKTNNFTKTISQEFIVCG
                     IITTLPVTSQVGAEFGGVISTNQFNKFTGISSYERFDIWTSKLANDKYVESELNKITE
                     KTNKGILIPYKAETAEYEKIDNQKTLIMVLVTGIIVILSLFNCCNTIVTSINSRSREF
                     ALFRGIGISKHEINKIVKLESYIYIIVSFCISIIPILIVRSIIIKPFESINLINWKFI
                     GAIILIVFILVSIIMITTLKTLNKIQNENFMEEIKTL"
     gene            4374..4718
                     /locus_tag="KRH54_003279"
     CDS             4374..4718
                     /locus_tag="KRH54_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580909.1"
                     /translation="MYCLSVVLAIYGIYVFINCTEYIYTMLSTKHLTVKGNEYTIINY
                     YMSNFSQYIIFAILLASMGFVIQKIVHMHFSEIELSGNYSTTVRNISDISLDNDNEMF
                     NRGNIENSILKK"
BASE COUNT         2113 a          425 c          704 g         1548 t
ORIGIN      
        1 aattaaaaat ataatgaaag tttagtttta attcatttat ttaataaaca tttcaagtaa
       61 aataatgttt aaatcagaaa tataaataaa tattattata atcgttatta aagaccaaaa
      121 atcccacctt agaagtttgt atcatatgaa acctttatag attatgaaag caataatata
      181 gtgtatgata tataaaataa gttataaaag tattttttta gatattcaaa aagacattgt
      241 aagttatatt aataatatgg gtatcaacga attttggata taatgagttt ttcaacttaa
      301 agttatgtaa taatgaatgt aaagaatatt taatgaaatc attttgttgg ttatgtctag
      361 ctgaatacaa taatctttta gataaagatt ttatatttga agataggtgt gaaaaaatta
      421 agaagatatt aacatcagca gtttagaggc aaagcatgta aagtaaagtc tgggtaacta
      481 aaagtagtaa tttagattgt aactattgtt ataaatatga aaatgaggaa acaggaaata
      541 taagttagga tgtagaaaat aggttactaa ataaaaagct taattatata atcaaagaaa
      601 taggtatata ctgtacaatt tcatggtgaa gaacttttcc taaataaaaa ggttggtaaa
      661 agagatataa gaaataaaat tcaattataa gatatggtat gataactaaa ggcactatat
      721 ggagtgaata ggcggaagaa ttttttgaaa attataaaaa tagtttctgt tgctacatat
      781 caataagtat agatagagat tgcaaaatcc atagcaaaaa tcgaatttat aagtgtaaat
      841 aacatgtaag acaaggagta aattatgagt attttagaga taaaaaacct aaataaaata
      901 tatggtaaat cggaaacaga ggtgaaagcg ttaagtcaaa taaatctaaa aataaatagt
      961 ggagaatttg ttgtgatagt aggtaaaagt ggaagtggta aaagtacttt actacacata
     1021 atggcaggac ttgaaactcc aacaaatggt gatgtgatta ttgataatat aaatatatca
     1081 tctcttgatg aaaagaaaag atctgcatta cgtaaagaaa agttgggatt aatatttcaa
     1141 tcatataatt tgattccagt tttaactgtt gaagaaaata taaaactacc aactataaat
     1201 ataaaaaata aagatgaagc ttatattaat gaattgatgg agttactagg aataaaagat
     1261 agaagaatgt atttgccaaa tcaattatca ggtgggcaac aacaaagagt agcaatagca
     1321 agggctcttg taaataaacc atcaattgta tttgctgatg aacctactgg aaatttagac
     1381 acaaagacag agtcagatgt attaagttta ttaaaagaat ctcaaaaaaa atataatcag
     1441 acaataataa tgataacaca taacatagat ataacaaaat atgctgatag agttatagaa
     1501 atagaagatg gatttataag agagtaggta gatttatgaa acttaattat attgtaaaaa
     1561 aatatataat gggaaataag aaaaatacat tattaatagt tgtaagtatt gtaatttcaa
     1621 cagcgttgtt tttaattatg aatataataa gtgaagatgc gacaaactta atgataaatc
     1681 aagctaaaaa tgaatttaca ttaaaacatg cacaatatat caatccaaaa gacgaagaaa
     1741 taaaatatat agaaaataac acaagtatag ataaagttgg aaaatcaatg cttttaggaa
     1801 ttagtgatat tggtaaaggt caaacactac aaattctatc tcaggataaa gttgctgaag
     1861 aactaaatga tatttacaca ctagaaaaag gtaaattacc agtagctgaa aatgaaatcg
     1921 ctatagattc atggtatatt aaacaaaaaa agataaaaaa ccctatagga aaaaggataa
     1981 cattagatta tagaagacaa gggatagatc aagaagattt atatactggg gaaaaagaat
     2041 ttaaaataac tggtatctta aatagtaatc ctattttaaa agctcaagga acatcaattg
     2101 gccttataag taacgattgt gctatcaaga atattcctac taaaaataaa tatgatcaag
     2161 ttatgtttac ttttaaaaag gaaaaaaata tacagagaca atcacaaaaa cttattaaaa
     2221 atggaaattt gaatgagaat aacatttatt ttaataatga attattaata gctatatctg
     2281 atagtatgaa tttaaaaata ccttatataa tagttaatat agtactagca ctagcaacta
     2341 tattattaat ttataatatt ttttatatat tagtatcaaa tagaataaag gattttggga
     2401 tatttagggc tttagggttt aatccaaatg atatttttaa aataatgatt ttagaagtat
     2461 ctatatactc tattataagt atttctatag gtctaatttt aggtgggata atagctagtt
     2521 tatcaaggga gtatgttata ggagtaatat acaatgtcaa ctatataaat tctataaaaa
     2581 accagaacta tataaacaca tacataattt ctatattatt aagtttagga actataataa
     2641 tatcagtttc aaaacctctt atgttatcag ttaaaactga tccaatgatt tgtataagaa
     2701 gaaataatga aaaagtggat ataaaacaaa attcctttat aaataaattt atgataaaac
     2761 ttttcaaaga ctatggtaac atagcatcaa aaaatataca aagaaataag aaacgtacaa
     2821 atttatctat agcatcaatg gtaattatat ttttcttaat ggcaacaata tacacaaagt
     2881 ctactagtaa ctttttaagt gatggtgtat taaaatattg gatacaagga gattatttat
     2941 tacataatat tgatataagt actatacgtt caaataataa aggttatgat aaaaatgtac
     3001 tacaagagat aaaggcaatt gatggtgtaa caaaagtaaa ctcatatagg cataagtggt
     3061 ttaatataaa aattgatgat aaaagtataa ataagaactc tgattattgg aaaaaaaata
     3121 aagataatat tgaagctaga tcagaagtaa aagatggaat caaggtatat aataattctt
     3181 ttgaattttt aggaatagaa gatacagata tactagagga tttcttgata aatggaaagg
     3241 aaaatataga taaatttagt aaacaaccat atctgtatat aacaaagaaa tccagtgatt
     3301 cgcttaatat aaaaaaagga gatagaataa aagtatactt tgatattata gattcaaaaa
     3361 caaataattt taccaaaaca atatctcaag aatttatagt atgtggaatt ataactacat
     3421 taccagtaac ctcacaagtg ggagctgaat ttggcggagt aatatcaaca aatcaattta
     3481 ataagtttac aggaatatca tcttatgaaa gatttgacat atggacgagt aaactagcca
     3541 atgataaata tgttgaaagt gagttaaaca aaataacaga aaaaacaaat aaaggaattt
     3601 taattccata caaagctgaa acagctgaat atgaaaaaat tgataatcaa aaaacactaa
     3661 ttatggtatt agtaactgga attatagtta tattatcttt atttaactgt tgcaatacta
     3721 tagttactag tattaatagc agaagtaggg aatttgcact atttagagga ataggaatat
     3781 caaaacatga aataaataaa attgttaaat tggaaagtta tatatatatt atagtaagct
     3841 tttgtatatc tataatacct attttaattg taagaagtat aataataaaa ccttttgaaa
     3901 gtatcaattt aataaattgg aaatttatag gggctattat cctaatagta tttattttag
     3961 tatctataat catgataact acactaaaaa cattgaataa aattcaaaat gaaaatttta
     4021 tggaagaaat aaagacttta tagtgaacag cctataaatt aaagaggttt tatatgaaat
     4081 taattgcaat tatcgataat ggtgtagata ttgattttaa ataatttaga aataaaaata
     4141 aaataagtta tccaagttat atgtatggtg taataggggt acaatgtata gcaaatataa
     4201 atattggctt aataaaactc tactcttaag tctactgtcg ttttaacaca agactaataa
     4261 taagtgtaac ctaaaaatta ttgataatac aataaccttg tttatgataa ataacaaata
     4321 ttaagtgttg taaagatagt aagtttaaaa aaatatcaaa gatttcaatc acaatgtatt
     4381 gtttatctgt agtgttagct atttatggta tatatgtatt tataaattgt actgaatata
     4441 tatatactat gctatctaca aaacatttaa cagtcaaagg aaatgaatat accattataa
     4501 attattatat gagtaacttt agtcagtata taatatttgc tattttactt gcatcaatgg
     4561 gatttgtaat acaaaagata gtgcatatgc atttcagtga gatagaactt agtggtaact
     4621 attcaacaac tgttagaaat ataagtgata tctctttaga taatgataat gaaatgttta
     4681 atagaggcaa tattgaaaat agtattttaa aaaaataaat aatataaata tataaaatgt
     4741 gattgaataa agcttagaat acttagtgta ttttaagctt tttttattat
//
LOCUS       DAEOSP010000239         4784 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.243, whole genome shotgun sequence.
ACCESSION   DAEOSP010000239 DAEOSP010000000
VERSION     DAEOSP010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.243"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            47..850
                     /locus_tag="KRH54_003280"
     CDS             47..850
                     /locus_tag="KRH54_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580910.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            862..1992
                     /locus_tag="KRH54_003281"
     CDS             862..1992
                     /locus_tag="KRH54_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8580911.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            1996..3417
                     /locus_tag="KRH54_003282"
     CDS             1996..3417
                     /locus_tag="KRH54_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580912.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYIIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDGEEDDSDKYGDIEI
                     DYSPERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPW
                     NLYLNRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEF
                     KQAIKEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYA
                     NNKKINLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTN
                     DEILEINDVRNDGYSMNSQAPIL"
     gene            3628..4137
                     /locus_tag="KRH54_003283"
     CDS             3628..4137
                     /locus_tag="KRH54_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBG8580913.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         1987 a          406 c          845 g         1546 t
ORIGIN      
        1 ataattaata tagatataaa aataattata gaaggaaagg cacattatgg aaaaattgag
       61 agttattgaa atgataaaaa ataatcccaa tattatagct acaatagata atccaactga
      121 tgaaatgaaa ttgttagcta ttaaagaaaa tgggcttgta ctagaatata taaataatcc
      181 aaccagagag atgcaagaac tagcaataga taataatatt agagcaatta aatttataga
      241 taatccaaca gaagatatga tgataaaagc agtaaatgag ggatggagta tactggatta
      301 tatcaaaaac ccaactgata aagttattga aatggctata aatcaagcag gatgggcaat
      361 taaatatgct aaaaatccaa gtgaagaatt acaactgtta gcagtaagaa aaaattatga
      421 ttcgataaag tttataaaac aaccttatga aagtgtacaa gaggaggcag tgagaataag
      481 ttatgatgcc ttgagatata tagattcacc tagttataat gcagaactta tagctataaa
      541 aaataatgaa gcagctatta gctttataac tgatttagat aaaaataaaa tgttggaatt
      601 tttaaaagtg aatattttag tgattaagta cattctgaaa aaagtatcta aagatgaatt
      661 agagagtgta ttaaaagaag tattatccaa agaagatgtt gaggaaaagt atattagaga
      721 ttttttgaat tgtagtgtta tagatagaaa tagcgaaagt ataccaatag ataaaattat
      781 gtttatttat aaatatggaa gtaagacagc aaaaaaaata gctgtagatg agaaattaaa
      841 gattatgtag gagagagaaa aatgaatttt atagacacat taaaaagcgt tgacttaata
      901 ttatccactg gagaagtacc tttaatagta ggtgaaagtg gaataggaaa aacagcttta
      961 gcaaataagc ttgccaaaga aaatgattgg agtttaattg ttattgatgg aaacttactt
     1021 aaagagggtg aaataggtgg tcttccaact atagaatctt atgtaggagt taattctaat
     1081 ggatataaga ctgaaaagaa aacaacagtg tatgctgttc ataacaaatt aagagaaatt
     1141 gatgaagaga tatctaaaag taagacagtt cttttattta tagatgagat aaatcgttgt
     1201 gagcatacag tacagcaaga acttatgaat ttaattttaa atagagaaat taatggatat
     1261 aaactgcatg atgatgtaaa gattttagca gcaatgaatc catcaagtaa atatggttca
     1321 gattttgatt atcaagtggt agatatggat gcagcccaag aaaataggtt tgtttggtta
     1381 aatatggaga gtgaccatac acaatggata aaatgggcaa tagatgaggg tattgaaaga
     1441 aaagttatcg agtttatttc cacttttcca gaatatctgc ataaaataaa tgaagatgat
     1501 gtaagagcca ctcctagaag ttatgagaga gtatctaaaa tttataaagt ttataaagaa
     1561 aaaaataatt ctattcctag agcagtattt ttaaatgttg taaagggtaa tgtaggtaaa
     1621 gttattgcag aagaatttat aagttttatt gaatcaaact ctgaaccatt aatatcctat
     1681 gaagatgttt ttttaggaga atctattgat gagtctattg tagaaagagt aaaaaatgag
     1741 agtcatacaa gactttatct atcagctatg aacattctaa aagacttaga attaaatatt
     1801 aagaatgata aatatgaatc aaaccactat attaatagat ttattgaatt tttaaaaatg
     1861 tatcctgtag atttaatgat aggtattatg aaggatatta gaaacagtta tattgaagta
     1921 tataaaaaag ctatagaaaa tgaagagttt gtaaaatcat attttgaatc ttatagttta
     1981 ataaggggat aatgtatgga aaattatttt gataaacaag caaaagagct ttataataaa
     2041 gcaagtgaaa ttataaatac tcattctatg ctaaaggcaa atcgtgctaa tgagaaattt
     2101 gatatagata taccacaaga ttttaagtat gagtttttta gtcttgtaga taaagtgaat
     2161 ttaagtctta tggaagaaga agataatttt tatggatatt ttttgtttca gatgtcaaga
     2221 gaaataagat ttgatataag tagtcctaca ggtgtgaatt ttaagggagc taaatatatt
     2281 atatatttta atccaataat ctttttaacg cttgacataa agcaaatgga gactacaatt
     2341 aaacatgaaa ttcatcatat attatctatg catttgatga gagcaaagga gttaaaagga
     2401 aagtatagta cattagcaat taatatggca atggatatag tagtaaataa atttctaaac
     2461 aatttaccac catatgcaac taccttagag tgggtaaatt taaaatattc cttaaaactt
     2521 gaaccatatg aaccttttga atattatgta gaaaagattc agactgaatt agatttgatg
     2581 gaagtggatg aagatggtga ggaagatggt gaggaagatg atagtgataa atatggagat
     2641 atagaaattg attatagccc cgaaagaact catgatatat gggatgaatc caatgagata
     2701 gatgaaaata cccttagaga atttactgaa aaatttatta acagttctca aaaaggtaaa
     2761 gttcctgctt atttagaaag tatgatatca tcacttaaaa atagtaaggg tgaattgcca
     2821 tggaatttat atcttaatag aataatggga acagttgaaa ataataaaaa gaagactata
     2881 acaagaagaa atagaagaca accaaatcgt ttagatttga ggggagaact tagaagtcat
     2941 aagtcagaaa tagcagttgc acttgatgta agtggaagta ttagtgatga agagtttaaa
     3001 caagctatta aagaagttct tagtatagtg aaaaactata atcatgaaat tacaattata
     3061 gaatgtgata atgaaattag acgtgtatat aaagtgagaa ctgaaaaaga tataaaagat
     3121 agaattagga taggtggagg tactagattt tctccagtct ttgagtatgc taataataaa
     3181 aagattaatt tgttagtcta ctttactgat ggtaaaggtg aaaataaact acaagtaata
     3241 cctagagggt acaaaatttt atgggttatt tctggaagag gagataaaat ttcattaaaa
     3301 gaaccttatg gagcagttaa aaaacttact aaggttaaaa caaatgatga aatattagaa
     3361 ataaatgatg taagaaatga tgggtactca atgaatagcc aagcaccaat tttataggta
     3421 ttcacaggag ataagctgta ttaatttatt ttgatatgct tgttataaaa atctaaaaaa
     3481 tacattaatc aatggttaat gtatttttta attttaataa tataagtttt acttacattt
     3541 aagtctcaaa atgtgttaaa atataatata tagcagatag ttttggcaag gtggatttag
     3601 ttgtcaaaac tatttttttt attaaggatg gtgatgtttt tgtttaagat tgatgattat
     3661 ataatgtatg gtatgacagg agtctgcaaa gtcatggata taacaaatga acgatttaca
     3721 aatggaataa aaaaagagta ttatgtattg agtcctattt attctaataa tacagtaata
     3781 aaaattcctg tagataatga aaaagtacca atgagaaagt tactttctaa aattaatgtg
     3841 ctttcattaa taaatgatat tcctaatatg gatacatcat ggatagataa tgaaaaattg
     3901 agaagtgagc aatttaaaaa aatactcaga ggtggtaaat gtgaagagtt aataaaatta
     3961 gtaagaagta tagataataa cagagagtat gtaaaatcta ttggtaaaaa aacacatcaa
     4021 gcagatgata atataatgaa agaagctgaa aggttattaa atgaagaatt tgcaactatt
     4081 ttagatattt ctcctaatga agttagttca tatatttcaa gtcatatacc tcaataaagt
     4141 gttttagtat cctttattta atctatttta agtattattt atgtatgctg gttttggtaa
     4201 aataaaatat ataattaaat tatataaaaa ataataatga ttgtattgaa gaattacaga
     4261 atagtgcgga gagcctactc ctttaggtgt gagatggata tcacttaaag atttaaattg
     4321 ttattgtaga tgtttttaat ataaaacaag attaataagt gtattgtaag ctttaagtga
     4381 aattgatact ataatgagtt acttagattt taaaatatat aatattataa aaaatccttt
     4441 taggttttga atgaataaga tggatactat gttgaataga atccatctta tttaagtttt
     4501 atttatgttg tttatttgta agcattattt ttgtacatga aaacacagtg taaaaatatg
     4561 cttagaatta tgaagtttta ttaaaaagat attcgacaaa ataagactaa aaatattctt
     4621 aaacttataa ctaagataaa tattgtatat ttatagtgta gcctgaaaaa ttgtggatgt
     4681 acgaaattta tttagtattt tattgaaaca aagtagaaga agtactatag tttatctttg
     4741 aatagattgt tattttttag tgaaaaagtt attaggttaa taca
//
LOCUS       DAEOSP010000240         4673 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.244, whole genome shotgun sequence.
ACCESSION   DAEOSP010000240 DAEOSP010000000
VERSION     DAEOSP010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.244"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            638..1945
                     /locus_tag="KRH54_003284"
     CDS             638..1945
                     /locus_tag="KRH54_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580914.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYYNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEVFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    2031..2293
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2419..3222
                     /locus_tag="KRH54_003285"
     CDS             2419..3222
                     /locus_tag="KRH54_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG8580915.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            3260..3871
                     /locus_tag="KRH54_003286"
     CDS             3260..3871
                     /locus_tag="KRH54_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG8580916.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3903..4574
                     /locus_tag="KRH54_003287"
     CDS             3903..4574
                     /locus_tag="KRH54_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG8580917.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
BASE COUNT         1881 a          425 c          832 g         1535 t
ORIGIN      
        1 attatctagt tacaattatt tataactaag ataataaaac aacaatggtg ctaatttata
       61 tgtataaatt aattatgaaa aatttatata agtaaaatat attaacataa aggtaaaatt
      121 ttgtatacta aaagggaata tttacagttt aatggcagtt ttaaaataat aaattaaagc
      181 tgtcattttt atttaaaaat ttttagaaaa agtacaatag tgttaatgtg tgaattagat
      241 aaggtgtata taatagttta gatgatatga tataaaatat gttgtaaatt aaatgttttc
      301 tttgacaata tatacacaag gatgataata tctatatgaa taaaagatag tgtgtaattt
      361 gataatatgt aattataaca ttatgatata attacaataa agtatgtaat aatctgtaga
      421 aatttcaagc tagttatcta agttttcgga taactaaatc taaacggaat acataatgta
      481 ttaaataaat gatgatacta aaatgatact atttttatag aaatttattg aggtatataa
      541 ttaatgaaaa taatatatta aattatataa attaaattat ataaattaaa ttatataata
      601 tattgagttt tacataaaag caaaggtggg aataaaaatg aaagaaattg ctgatgctat
      661 aacatcagag tatttaagct atggtatcaa tgaagatatt agtatctatg aaggtagatt
      721 ttgcatatat ttagataaaa aatatagatg taatgggaaa atttattata aaatgacacc
      781 tccaatatct ataagtttta aagcagacat aggttgtgtt gaagagatag acaatgaaga
      841 tgacaattta gctttagatt atgataatgc tattcttgaa gttcatggat ataaaattat
      901 ttctataaca ataaatactt taagtgaatt tagtgtagaa ggttatatca atgatgactg
      961 cataaaatct aagaactcct atgttgaata tgtagatttt aatataataa acttagataa
     1021 aatacctgga aaactcatta aatacaatga taaggtatac gcaggtagaa ttgaatttga
     1081 tataaatgat tatgttgtaa ctatagacaa gcgatatgat tatagaaaag aattaaaatc
     1141 agaattaaag tctaaatctg gagcaataat aactcatata ggtaggatta gaagaaaaga
     1201 tggtagaatt tttagaacaa ataatacaat aaatctttta gataggatat caacagcttt
     1261 gagttttatg tgtggaagat atgtagggtt ttgtttggcc aaagggtata gaagtggtaa
     1321 tgaagtttat agaatatgga atgaaaatca aatatctcct tttagatatg taccaacatg
     1381 gtcagataca ctttcaaatt attataatat ggaaaaatat ataagcctta tgtgtaaaaa
     1441 gctagaagat ttttattatg gttctgctat aaagagtgtt gttgattggt acatagaatc
     1501 tcttggaagt gctaccatgg aaaacaatat aatatctgta caaattgcac ttgaaacttt
     1561 gtcatatgta attctagtgg aacaaaataa gatacttaca gatgaagtat ttgactgtaa
     1621 tttggcatct aaaaatataa gactcttact tgatacatgt aagataccat atggtaaaca
     1681 tgaattaaat atatttgata atataataaa aaataaattt gatgatggag tggatttagt
     1741 tatttattta agaaatagca tagttcatcc aagtagaaag actcatagag ctgtgttaga
     1801 ggttgaagat atttggaata taatttcaat aggaacgaga tatattgaac ttgttttact
     1861 ctttatttta ggttatagag gagaatattc aaacagatta gtagaaagat gttatggtga
     1921 agtggaagtt gtaccttgga actagatttt tatatttatt ttaaaatata tattgacata
     1981 tattgcctta tgctataaaa tcaatatcat aataataatt cagaataaca ttaaatgcta
     2041 agaaaagaaa tagtaaaagt agttaatctt aaacagagag tttccgattg gtgagaggag
     2101 caatagattt tactttgaac acatctttga gcaggactct gaaagagtag aaatacttta
     2161 ttaggagatt tccggaatcc ttgcacccgt tatagtgcta gagtataata attttattgt
     2221 acttaaagag gttagtgtcg tgaggcatta atgaacagag gtggaaccac gagagttaac
     2281 tcccgtcctc tagcagaagc tagaggcggg agttttttaa tataattttt taagcgcgca
     2341 gagaattata ttgcaataga tacacttttt aaaaaataat caaaatattt taaaaattaa
     2401 aaggcgaggg agaatattat gaaaaatata ttaaaaaaag taggaatatt tacaattatg
     2461 ttaggtttat taggtggagt tgtaggttgt tcaaaaccag ataatgaaaa agataaggat
     2521 gcttcaaagg aatctaaaaa agaagtagtt gtagggtttg ataatacgtt tgtaccaatg
     2581 ggatttttag atgaaaaggg agatacagta ggatttgacg tagacttagc taaagaaaca
     2641 tttaaaagac ttggaatgga agttaagttt cagcctatag attggtcaat gaaagaaact
     2701 gaattaaatg attcaaaaac tgtagatgta ttatggaatg gatattctat aacagacgaa
     2761 agaaagaaaa tagtatcata tacagaacca tatttacaaa ataagcagat aatagttact
     2821 ctaagtgact caaaaataaa ttcaaaagct gatttaaaag ataaagaagt tggaacacaa
     2881 caaggttcaa cagcacttga tgcagtggaa aaggataaag attttatgaa tagcttaaaa
     2941 ggtggagcac cagttcttta tgatacttat gataaagcac ttagagattt agagataggt
     3001 agaactagtg cagttgttgg tgatgaagtt ttaattagat actatatggg acaaaagggt
     3061 gaagacaaat acaaggtatt aaaagacgat tttggattag aagattatgt tgtagcaact
     3121 tctaaagaaa atccagaact ttgtgagaaa ataaatgaaa cattaaaaga aatgaaaaaa
     3181 gatggaactt ttgacaagat ttatgataag tggtttaaat agaagataag ttagctgtta
     3241 agaagggggc actaaaggca tgttacaagg tttagagata gttatagcaa tgttttgtat
     3301 aacattgata gtatcaattc cattggggat tggagttgca tttttaagat tatcaaagaa
     3361 taaattagta agtggtatta cacaatgtta tatattgata atgagaggta cacctttact
     3421 tttgcaaatg atagtcatat tttacggatt acctctttta ggaattgtat ttgacagatt
     3481 tacagctgga gtagtagctt tcttcttaaa ttatgcagca tattttgcag aaatatttag
     3541 aggtggaata cagtcaatcg atagaggaca atatgaagct tcaaaagtac taggatttga
     3601 taagtttact atgtataaga gagtaatctt tccacaagtt tttaaaagaa tactagcacc
     3661 tatttcaaat gaagtaatca cattagtaaa agatacatct ttagtatata tattaggatt
     3721 aaatgatatt ttaagaatat ctcaaatagc aatgaacagg gaagcaagtc tgttaccatt
     3781 atttgaggca ggagcgatat atcttatatt tgtagctata cttactaagg gatttgaatt
     3841 acttgaaaaa aaatattctt attatagata aagttacaat attataggag gaggtttttt
     3901 atatgttgaa aatcaaaaac ttaaataaat catttaaaaa aaatagagta cttaaagata
     3961 tttcttttga gttagaagaa ggtcagattg gagttttact tggtaagtct ggtgcaggaa
     4021 agaccacaat attaaggtgt ataaatggtc ttgaagaatt tgatagtgga gagattataa
     4081 tagataatga ggtaataaaa aataagagag atatggcaaa aatacgagga aaaataggta
     4141 tggtttttca aaatttcaac ttatttcctc atatgacagt ccttgaaaat ataattgaat
     4201 ccccagttaa tgtttttaaa gttcctagaa aagaggctga agagagggct agagaacttt
     4261 taagattagt tgatttagaa gataaattaa attcttatcc atttgaattg tcaggaggac
     4321 agcaacagag agttgctata gctaggtcat gtgcattgat gccaaaagtc ctttgttttg
     4381 atgaacctac ttctgcactt gatattgata ctattcaaag agtagtcaat ataatgaaca
     4441 gacttaaaga caaaggtatg acaatattaa taattacaca tgatgttgtc ttctcaaata
     4501 atgttgcaga taaaattata agtataaaag atggtatagt agaaaatgtt caaataaaag
     4561 aaaaaattgt ttagagtttt ataaatataa actagggtag aattttataa tataaataaa
     4621 gggcaagtaa aatcaaatat actatgccct tttgttatct caaaatttaa tct
//
LOCUS       DAEOSP010000241         4643 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.245, whole genome shotgun sequence.
ACCESSION   DAEOSP010000241 DAEOSP010000000
VERSION     DAEOSP010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.245"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            151..2562
                     /locus_tag="KRH54_003288"
     CDS             151..2562
                     /locus_tag="KRH54_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HBG8580918.1"
                     /translation="MRKYKSKNLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMNLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETN
                     IDAYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            2859..4436
                     /locus_tag="KRH54_003289"
     CDS             2859..4436
                     /locus_tag="KRH54_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBG8580919.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
BASE COUNT         1876 a          548 c          850 g         1369 t
ORIGIN      
        1 taaatcaata tatcttaaaa tattataaaa ataaaactat aatattctat aattaatatg
       61 tactcataat ccatatttta tcaaaataaa atatgacctt aatttagaaa tctgatataa
      121 taaaaattaa atacatataa gggggtaaac atgagaaagt acaaatcaaa aaacttgtca
      181 aagctactag cattgttgac agtttgcttt ttaatagtgt caacaatacc tgtctcagca
      241 gaaaaccata aaactctaga tggagtagaa actgcagagt attctgaaag ctatcttcaa
      301 tacctagaag atgtcaaaaa tggagacaca gcaaaatata atggagtaat accttttcca
      361 catgaaatgg aaggtacaac acttcgaaat aagggtagaa gtagtcttcc atcagcatat
      421 aaatcaagtg tagcatacaa cccaatgaat cttggtctta ctacaccagc aaaaaatcaa
      481 ggaagtctta atacatgctg gtctttttca ggtatgtcaa cgttagaagc atatcttaaa
      541 ttaaaaggat atggaacgta tgacttatca gaagaacatt taagatggtg ggcaactggt
      601 ggaaaatatg gatggaactt agatgatatg tcagggagtt caaatgtaac agctatagga
      661 tatctgacag catgggcagg tcctaagtta gaaaaggaca taccatataa tcttaaatct
      721 gaggcacaag gtgcaactaa accttcaaat atggatactg cacctactca atttaatgta
      781 acagatgttg ttcgtcttaa taaagataag gaaactgtaa agaatgctat aatgcaatat
      841 ggttcagtga catcaggtta tgcacattat tcaacatatt ttaataaaga tgaaacagca
      901 tataactgta caaataagag agctccatta aatcacgctg tagcgatagt aggatgggat
      961 gataattatt caaaagataa ttttgcatct gatgtaaaac cagaatcaaa tggagcatgg
     1021 ttagtaaaaa gtagttgggg agagtttaat tctatgaaag gattcttctg gatttcttat
     1081 gaagataaaa ctctactaac agatacagat aactatgcaa tgaaatcagt atcaaaacca
     1141 gatagtgata aaaaaatgta ccaacttgaa tatgctggtc ttagcaagat aatgtcaaat
     1201 aaagtaacag cagcaaatgt atttgatttt agcagagact ctgaaaaact tgattctgtt
     1261 atgtttgaaa cagactctgt aggagcaaaa tatgaagtat attatgcacc agtggtaaat
     1321 ggagttcctc aaaacaattc aatgacaaaa cttgcaagtg gaacagtatc atattctgga
     1381 tacataaatg tacctactaa ttcttacagc ttaccaaaag gtaaaggagc aatagtagta
     1441 gttatagaca acacagcaaa tcctaataga gaaaaatcaa ctttagcata tgaaactaat
     1501 atagatgcat attatttata tgaggctaaa gcaaacttag gtgaaagcta catacttcaa
     1561 aacaataagt ttgaggatat aaatacatat agtgaatttt ctccttgtaa ctttgttata
     1621 aaagctataa caaaaacatc ttctggacaa gctacttcag gagaatcttt aactggagca
     1681 gatagatatg aaacagcagt taaagttagt caaaaaggat ggacttcttc acaaaatgca
     1741 gtattagtaa atggagatgc aatagtggat gctttaacag ctacaccatt tacagcagca
     1801 attgactctc caattctttt gacaggaaaa gataacttag attcaaaaac taaggcagag
     1861 ttacaaagat taggaactaa aaaagtttat ctaataggtg gagaaaattc tttaagcaag
     1921 aatgtacaaa ctcaacttag taatatgggt atatcagtag aaagaatttc aggtagtgat
     1981 agatacaaga ccagtatatc tctagctcaa aagttaaata gtataaaatc tgtttcacaa
     2041 gttgcagtgg caaatggtgt aaatggactt gcagatgcaa taagtgttgg tgcagcagct
     2101 gctgataaca atatgccaat aatacttact aatgaaaaaa gtgagttgca aggtgctgat
     2161 gagtttttaa attcatctaa aataactaag tcttatataa ttggtggaac agctacttta
     2221 tcatcaaatt tagaaagtaa gctttcaaat ccaacaagac ttgcaggcag taatagaaat
     2281 gaaactaatg ctaagataat agataaattc taccctagct cagatttgaa atatgctttt
     2341 gttgttaaag atggttcaaa gagtcaagga gacttgatag atggactagc agtaggagca
     2401 ttgggtgcta aaactgattc accagtagtt ctagttggaa ataagttaga tgaaagtcaa
     2461 aaaaatgtac ttaagtctaa gaaaatagaa actcctatta gagttggtgg aaatggaaat
     2521 gaaaatgctt ttaatgaact aaatactctt ttaggaaaat agtgaatgga aagatagtaa
     2581 ggatatacat tttattatta aaaaatgttc aatagaagtt tataaatata aaataacaaa
     2641 aaggggtgtc tcaaaatgaa ctttttagtt catgaggcac tcttttttgt tgttttatat
     2701 ttttatcatt taaataatga aaaataggat tgtctatatt tttagatagt tttcttatta
     2761 ttgatgaaaa atgtaataat tttaaactgt aaaatagtgt aaagttattt tataatataa
     2821 atatagtaag attttaaaca aaataagggg gaataataat gaaagcacca aaaactattt
     2881 taacaatact gacaatagcg cttactttaa gtagcatttc tataatacca tcatatgcac
     2941 ttacagagga aaaattaata ggtaatggta gatatgaaac tgctgtaaag ataagtcaaa
     3001 aggcatacag ttctagtcct aatgtagtgc tagttaatga caattcatta gctgatgcac
     3061 tttcagctac accatttgca aaggctaaag gagcaccaat acttttgaca gaaagtgata
     3121 agctagatga taggactgaa aaagagataa aacgtctagg agctaaagat atttatttga
     3181 taggaggtac tgctgtactt aataaagata tagagaacaa gttaaaaagt aatggactta
     3241 atgtagaaag aattaatggt aaggatagat atgaaacttc actgattttg gctaataagc
     3301 ttaaggatat aaaagatatt aaggaagttg ctgtagtaaa tggtgaaaaa ggattgagtg
     3361 atgctgttag cgttggggca ccagcagctc aaaataagat gcctataatt ttgtctagtc
     3421 ctaaggatgg agtagaagca tttgataaat ttataaggga tgagaaagtt ataaaagcat
     3481 atgtaattgg aggtacaaat tctgtttcaa gagctgtaga aaagagtctt cctaatgcag
     3541 aaagagtgag tgggaaagat agaaatgaga ctaatgctaa agttatagaa aagttttaca
     3601 ctgatacaaa tttaagtaat ttatatgtaa ctaaggatgg tagtaaaaat gagaatcaac
     3661 taatagactc gttagctgtt ggcgtattgg ctgcaaaaaa tgagtctcct gttgttttgg
     3721 ttggtaataa attaaacaca aaacaaagag atatacttag tactaaaaaa ttaaatacta
     3781 taacacaggt aggtggaaat ggaaatgaag aagcttttga tgaaattaaa agtctacaag
     3841 aaaaaactgt gtttgaagca aaaactgttg aggaattaac tgatatgata aatatagcaa
     3901 gtcctaatga tattataaac tttaaaccaa aagagaatac tgtaaatgaa gcttttagaa
     3961 tggtaacaaa taaaccaata actgtgaata taaagggtga ttgttcaaag actcttacag
     4021 tagatatgcc taatggtgaa gtgaataact atgctacttt ggtaaatgtt atagttagaa
     4081 atattggaga aggtggattt aataatcatg atacaatcac tatattgtca gttcgtgaca
     4141 aaaatggtag agttatagaa aatactagaa attcagatat agatacgttg atgatattag
     4201 cttctgctaa tgatacaaaa ttgattaatg atggatatat aggaaaactt atagataatt
     4261 catctaatag tgatataact aataatggta ctatagataa aaaagttaat caggttgaag
     4321 atttagaggc taaagtagat tcaattgaaa aagctataga ttctataagt caaaaagtca
     4381 ataaaataca agatatacta gataagcttg gatttctaaa gaaatttctt agctaagaat
     4441 atgtatattt aagtgaagtg tgtgttgtaa ttgttctaaa gttatgtagt taaacaggtt
     4501 tatatacaaa tagatttata gataaatatg tagtttagta tatatttgaa taagctttgg
     4561 ggaggtaaca tggggattgg aattgaatgt aacaaaaaaa taaataaaag tattgacaaa
     4621 actagaataa agaaaaataa tct
//
LOCUS       DAEOSP010000242         4621 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.246, whole genome shotgun sequence.
ACCESSION   DAEOSP010000242 DAEOSP010000000
VERSION     DAEOSP010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.246"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     misc_binding    124..386
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            462..3128
                     /locus_tag="KRH54_003290"
     CDS             462..3128
                     /locus_tag="KRH54_003290"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBG8580920.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
     gene            3553..4263
                     /gene="rgaR"
                     /locus_tag="KRH54_003291"
     CDS             3553..4263
                     /gene="rgaR"
                     /locus_tag="KRH54_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBG8580921.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT         1858 a          463 c          832 g         1468 t
ORIGIN      
        1 aaaacatatt ttaaaatata acaactatat aaaagagaat agacataatt aagaaaattc
       61 agaaaaaata aaataaaaat aattgacaaa gagaataata tttgtatata ataatatgta
      121 atattaaaga ctgagaagaa gaggagtaag taaatactac taaaagagag gaaaattcac
      181 tggctgtaag attttctaag tataagattt actgaatgta gctttggagt ttctaaactg
      241 aactataagt aagtttagac ggttagaacc gttaaattct ttgagagtca aagttgataa
      301 aataacatta accaataatt taattatatg ttaatgtatt ttaagctttg gaattaaggt
      361 ggtaacgcga gcttttcgtc ctttttaaag aggatgaaga gctctttttt atttctaaag
      421 atatttaaaa aatttattta taaaatacag gaggcaggac aatggaaaat acgaatttat
      481 caaaaactta taacccaaaa gattttgagg ctaggctata taaaaaatgg atggatgaag
      541 ggtattttaa atctaaacca aatccagata aaaaaccatt tacaataatg atgccaccac
      601 caaatataac agggcaattg catatgggac acgctcttga ccatacactt caagatatat
      661 taatcagatg gaaacgtatg gatggatatg aagctttttg gcttccagga actgaccatg
      721 cttctatagc aacagaagtt aaagttgttg aaagaataaa aaaacaagaa ggcaagacaa
      781 agtatgaaat aggtagagaa gaatttttaa agagagcctg ggaatggaaa gatgaatttg
      841 gaggaaaaat atctaatcag cttaaacaac taggtgattc ttgtgattgg gataaagaaa
      901 gatttactat ggatgaaggg tgtaatgaag cagttataga attttttgta agcctatatg
      961 aaaaaggtca catatacaga ggaaatagaa taataaactg gtgcccagat tgcaaaacta
     1021 ctttatcaga tgcagaagtt gaacatgaag aacacgacgg aaacttttat catataaagt
     1081 acccactaaa agatagtgaa gactttttag aaatagctac aactagacct gagacaatga
     1141 taggagatac tggtatagca gtaaatccag aagatgatag atataaacat ttaataggaa
     1201 aaacagcaat acttccatta gtaggaagag aacttccaat agttgcagat agttatgttg
     1261 atttagagtt tggaactggt gcagttaaga tgactccagc acatgaccct aatgattttg
     1321 aagtaggatt aagacataat cttgagcagt taaatactat gaatgaagat ggaactatga
     1381 atgaagtttg tggaaaatat gaaggtatgg atagatttga gtgtagaaaa gctatcgtag
     1441 cagacttaaa agaacaaggt tacctaatta agataaaaga gcataatcat aatgtaggaa
     1501 cttgttatag atgccataca gtagttgagc ctagattatc agagcaatgg tttgttaaga
     1561 tggaagaact tgcaaaacca gctatagata ttcttaaaaa aggagaatta gaatttgttc
     1621 cagataaatt tgataaaact tatttacaat ggctagaaaa tataagagat tggtgtatat
     1681 caagacaatt atggtggggt catcaaatac cagcttatta ttgtcaagag tgtggagaaa
     1741 tagtagttgc tagaaaaatg ccagataagt gtcctaaatg tggaagcact cattttaaac
     1801 aagatgaaga tgccttagat acttggttca gttctgcatt atggcctttc tctactctag
     1861 gatggccaaa taagactgaa gctttagatt attattatcc aacaagtgta cttgttacag
     1921 gatacgatat aatattcttc tgggtagtta gaatggcatt tgcaggtatg ttctgtatga
     1981 atgaaaagcc atttgaccat gttttggttc atggattagt aagagattct caaggtagaa
     2041 aaatgagtaa atctttagga aatggtatcg acccattgga gataatcgag caatatggag
     2101 ctgatgccct aagatttaca ttaacaactg gaaattcccc aggaaatgat atgagattct
     2161 atatggaaag agtagaattt gctagaaact ttgcaaataa attgtggaat gcatcaagat
     2221 ttgtatttat gaatattgat gaagatataa ttaaaaacat gactagagaa agtgttaaag
     2281 aggacttaac tctagctgat aaatggataa tatcaagagc aaataatatt gtaaaagaag
     2341 caactaataa catggataag tttgatttag gaatagcact tcaaaagata tacgatttta
     2401 cttggtcaga atactgtgac tggtatattg aaatggttaa acctagatta tatggggaag
     2461 atgcaaatgc taaatctgca gcattatata cattaactta tgttcttgaa aaaatattaa
     2521 aattattaca tccatacatg ccatttataa cagaggaaat atatactcat cttcctactg
     2581 ttgaaggatg tataatagta tctgaatggc caaaatataa tgaagaagat aacatggctg
     2641 aagaagaaga tatgatgaac ttattgatgg aaggtataag aagtataaga aatgtgagag
     2701 cagaaatgaa tgtaccacct tctaagaaag ctaaattgat aataatccca agtgaagaaa
     2761 aaatagaagc tattgagctt ggtaaagact attttataac tttagcatct gcttcaaatg
     2821 tagagatagc aaaagataaa tctaatgttc cagaagatgc agttggagta gttattgatg
     2881 gtgttgaaat atttatacct cttaatgaat tagttgattt tgaaaaagaa attgagagat
     2941 tatctaaaga aaagaaaaag ttagaaggcg aaataaagag agttaatgga aagcttgcaa
     3001 atcaaggttt cttagcaaaa gctcctgaaa gtttaattga agaagaaaaa gcaaaaaaag
     3061 aaaaatttga agagatgatt aaatctgttg aggaaagatt aacaaattta gaatctaaaa
     3121 taaaataaat ataagaatta gtaaaaacat acaaaaagta tatattctat actttttgta
     3181 tgtttttctt tatatatata caaatatgat gttataattc tttacagatg tgttatattt
     3241 ttattttatg ttgtgaatat atgaatttta tgtcttaaaa tttataagca ataaaaattt
     3301 gatatattat gatgtttaat atagggtaat aaatacatat tatatttatt ttactatatt
     3361 tttttacata atatatctag aaaagtatag gaatttatcg taatttaagt ttaaatatta
     3421 aagagaattt ctataagtag tttaatcgac aatttaaatt tataaagaaa tttaaatttg
     3481 aaagctagtg agtacagttt ggaaattgaa ataaagtaaa ctaaatgata ttttagattt
     3541 ggaggtgata ttatgttcag ggtagttata tgtgatgacg aaaaaataca aagaagcata
     3601 ctaaaagagt ttacagaaaa acttttgtct gaaagatgtc taaattatca aatactggaa
     3661 ttttcctgtg gagaagattt aatatccaaa tacccagaaa aaattgatat tatattttta
     3721 gatatccaaa tgaaagatat caatggaatt gaggtggcta aaaaaataag aaagtttgat
     3781 gaaaaagtag aaattatatt tacaacggct ttttcagagt atgcccccca aggttatgag
     3841 gttcgtgctt atagatattt agtaaagcct atagagtata gtaactttgc aatgggtgta
     3901 aatctttgta ttgataacct tcaaagaaag aaggagcgtt atatagtttt aaatagtaaa
     3961 aaaggattta atagaatact gatagattct attttatatg ttgaaactgt aaagagagat
     4021 ttagtcgtac atacaatagg tagagattat aaagctaata tgagcatgaa acaagcagaa
     4081 aaattactgt gtgaaaatgg cttttttaga tgtcatacaa gttttattgt taatttaaga
     4141 agaatagaag atcttaggga taatatgatt actattaatg aacaatttat acctataagt
     4201 aagtatagac ttaaagattt aaaagtagca ttaacaagct tgttatgtga tataatgtat
     4261 taattttaaa aaggtaattg aaaatatcgt aaaaaagatt taatgtcatc acagggctat
     4321 tgattgaaac tgtcttaata tataaaatgt tatttatatt aagacagttt tattatgcaa
     4381 aatagcagtt tatagatttt taatatggtt taacatgtat atgtagagtt tccattataa
     4441 tagcatgatt attaattagt agcttgatta tcaatatact attattgatt tttatatgta
     4501 ttatatgtat ttttagaata tgattacctt tttaaaataa tgactattta taaaactcta
     4561 tagatatttg tttcatttag ctatgcatac tttaattaat tatattaata tttaatggag
     4621 t
//
LOCUS       DAEOSP010000243         4518 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.247, whole genome shotgun sequence.
ACCESSION   DAEOSP010000243 DAEOSP010000000
VERSION     DAEOSP010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.247"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..2738)
                     /gene="carB"
                     /locus_tag="KRH54_003292"
     CDS             complement(<1..2738)
                     /gene="carB"
                     /locus_tag="KRH54_003292"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HBG8580922.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKEKPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYSTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEV"
     gene            complement(2931..3980)
                     /gene="carA"
                     /locus_tag="KRH54_003293"
     CDS             complement(2931..3980)
                     /gene="carA"
                     /locus_tag="KRH54_003293"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBG8580923.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(4237..>4518)
                     /locus_tag="KRH54_003294"
     CDS             complement(4237..>4518)
                     /locus_tag="KRH54_003294"
                     /inference="COORDINATES: protein motif:HMM:NF014225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580924.1"
                     /translation="NSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGND
                     STRDGFKIRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
BASE COUNT         1299 a          890 c          461 g         1868 t
ORIGIN      
        1 acttctcctg tagacttcat ttcaggtcct agacttactt caactttaga taatttagac
       61 attgagaata ctggtacttt tactgatact aattttggct ctttgtaaac tcctgtacta
      121 tatcctaaat cctttaattt agctcctaac atacacttag tagccaagtc aactattggt
      181 actccactta ctttacttat atatggaact gttctactag ctctagggtt aacctcaatt
      241 atatataatt cattttgaaa ttctataaat tgtatattaa ccataccaag cacattaagg
      301 tctaaagcta tttttttagt gtattctact attttttctt ttatttcatc agatatattt
      361 tgacttggat acattgttgt actatctcca gagtgaactc cagctctttc taaatgctcc
      421 attataccag gtatcaatat atcttcttta tcacatatag catctacttc tatttctcta
      481 ccagttagat atttatctat aagtactgga tttttatggt ctctatcaaa tgcatcatcc
      541 aaatactgaa ttagtttttc ttcgttataa gttatttcca taccttgacc accaagtaca
      601 taagatggtc taactaatac tggataacct aactccttag caatttcaac accttcatta
      661 gttgtccata caccttttcc ttttggtcta tttatatcta gtctctctag taaatcatca
      721 aatttttctc tatcttctgc tgcatctata tctctaaagt ctgttcccag tataggtata
      781 tttttttcat gtaaaaactt agccaatttt atagctgttt gtccaccaaa ctgtagtatt
      841 acaccttctg gtttttcttt ttctattata tttaaaactt cttcttctgt taatggttca
      901 aaatacaact tatcagatgt atcaaaatcc gtacttacag tttcagggtt attgtttaca
      961 attatagttt ctattcccat ttttcttaaa gattttacac agtgaactga acaatagtca
     1021 aattctattc cttgacctat tcttattgga ccagaaccca atactacaac ttttctctta
     1081 tcactaacta ctacctcatc atattgttca taagttgaat agtaataagg tgatagagct
     1141 tcaaattctc ctccacatgt atcaaccatt ttataaactg gttgtatatt atataaactt
     1201 ctcaattcat ataatttttc tgggcttatc ttcattaagt cagatatacc tttatcagaa
     1261 aatccctttt tctttaattc atgtaaatat tctttatcta agtcttctat ttttagaact
     1321 tttaactttt cttcttgttc tactatccat ttaaatttat ttataaacca cttatcaaca
     1381 ccagttattt ctgatatcat ttctacttta tagcctcttc ttatcatttc agctaagtca
     1441 aatagtctct catcatcagg tactactact ctagccttta attcttctaa tgttctacct
     1501 tctgatggag catgaaccaa tgaatatttt cctgtttcaa gtgatcttat acctttaaga
     1561 atagcagcct caaagttact tcctatactc atgatttcac cagttgccat catcttagtt
     1621 cctaattttc tattggcatg tctaaattta tcaaatggcc actttggtat ttttactacc
     1681 acataatcta atgttggctc aaaacaagca taagtttttt tagttactgc attttctatc
     1741 tcatccaaag tataacctaa agctatctta gctgctactt ttgctattgg gtatcctgta
     1801 gcttttgatg ctagggctga tgacctacta actcttggat ttatttctat tatcgcatat
     1861 tctaaactat gtgggtttaa agctatttga acgttacatc caccttgaac ctctattgca
     1921 tttattatgt ctattgatgc ctttctaagt agttgatact cttcatcact tagagtttga
     1981 cttggagcaa caactatact gtctcccgta tgaactccaa ctgggtctac attttccata
     2041 ttacatacag ttatacagtt tccatttcca tccctcatta cttcgtattc tatttctttc
     2101 caacctttta tactcttttc aagaagaact tgtccaacag gacttagctg tagtccatgt
     2161 gaaagtattt ctcttaattc ttcctcagta tcagctatac ctccaccagt tccacctagc
     2221 gtatatgctg gtcttacaac tactggatat cctatcttat tggcaaactc aagacctgct
     2281 tctaaatttg taactatatc actaactatt actggttgat ttatttcttt cattacttct
     2341 ctaaataaat ctctatcttc gccctttttt atagattcta tagatgttcc tataactttt
     2401 acattatatt tatctaatat accagcatca tgtaattcta cagctaagtt taatcctgtc
     2461 tgaccaccca tacctgcaag taaactatct ggtttttctt tttctattat tttttctata
     2521 aattctattg ttaatggttc tatatatatt ttatctgcaa tttctttatc agtcattata
     2581 gtagctgggt tactatttac taatacaact tcaattccct cttcttttaa tgcttggcag
     2641 gcctgtgttc ctgaatagtc aaactctgca gcttgaccta ttattattgg ccctgaccct
     2701 aatactaaag ttttctttat actatctaat ttaggcataa ctctttctcc ttatctaaac
     2761 aattttcaca gttcttatct tcatcatcta tacaatcaac tttaattact ttattttatc
     2821 ttaaattcaa atttactcat caatacatct atttaataca tctatgttaa ttaaaccaaa
     2881 ctttttgatt taagttattc ttgtttttta gttagctatt ctaatgttat ttattttact
     2941 aattctaaaa atttatcaaa aatatagtca ttatcttttg gtcctgggca agcttcagga
     3001 tggtattgaa cactgtatac atctagtttc tcatgtctca ttccttcaac agtattatca
     3061 tttaaattta tatgtgtcac tttcatttgt tcaggaactt ctgaaacata ataaccatga
     3121 ttttgagaag ttataaatac ttttccttct tctaaatctt taactggatg atttccacct
     3181 ctatgcccaa atttaagttt agcagttttt cctccaagag ctaaagctaa aagttgatgt
     3241 cctaagcata ttccaactat aggtttttta ccaataagag cttttatatt ttctataaca
     3301 tcttctaggt cttctgggtc tccaggtcca tttgacaaga atattaagtc agggtttata
     3361 ctcaatacat cttctggaga tgttgtagct ggaaatattg ttatatcaca tccacgagct
     3421 ataaatgatc ttaatatatt tctttttact ccaaaatcca taacagccac ttttggacca
     3481 tttcccttta tatgctctac ttcttttctt gtaacagttc taactgcttc tgtatttgaa
     3541 aacttatcca atttatgttt aacatcttct aatttactgt cttctaatgt aattatacct
     3601 ttcatggttc cattatttct aagtatctta gtcaaagctc ttgtatctat tccttcaagt
     3661 ccgataactt tattttgctt taaatattgg tctatatcaa tttcacatct aaaattactt
     3721 ggagaatcac ttttctccct tactatgaat ccctttactt gaactttttc agactctgct
     3781 gatgataaat ttataccata atttcctaca agaggataag tcatagtaac tatttgacca
     3841 tagtaagaag ggtctgttaa aacttcacca tatcctatca ttgaagtatt aaatactact
     3901 tctcctacac tttcttctaa atatccaaat gcctttccta taaaaactgt tccatcttct
     3961 aatattaacc ttgctttcat actacagtac ctcctataat tctctttaga caaactaaag
     4021 aacattaact aaatataagc ttttaaatat ctatatctat ttttcacttt tacattctag
     4081 ttttatactt atttacattt attttttatc tttttctatt atactacaaa tttcttaaaa
     4141 aaaagaagtt atctcaaaag taaaaactcc tagaaaaaat aacctctttt ttttatttaa
     4201 taattaaatt cttaaactat acaatcaact taatttctat tcagctatat tgtaaacttt
     4261 taactcatct ttatttaaat gtttcttctt aacttctact aaagctttta gtgtatccaa
     4321 agatgtcatt atttcagtag aaaattcaat agctgttctt cttatcttaa atccatctct
     4381 tgtagagtca tttcctttag ttggtgtatt tattactata tcaacttgtt tatttcttat
     4441 agcatctaat atgtttggtc tagattcttc tactctattt actatatcag cctctattcc
     4501 attttctctt aagctatt
//
LOCUS       DAEOSP010000244         4507 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.248, whole genome shotgun sequence.
ACCESSION   DAEOSP010000244 DAEOSP010000000
VERSION     DAEOSP010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.248"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            147..860
                     /locus_tag="KRH54_003295"
     CDS             147..860
                     /locus_tag="KRH54_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBG8580925.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVGLSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            978..1847
                     /locus_tag="KRH54_003296"
     CDS             978..1847
                     /locus_tag="KRH54_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG8580926.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(2002..3147)
                     /locus_tag="KRH54_003297"
     CDS             complement(2002..3147)
                     /locus_tag="KRH54_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBG8580927.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDIDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(3480..4256)
                     /locus_tag="KRH54_003298"
     CDS             complement(3480..4256)
                     /locus_tag="KRH54_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBG8580928.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
BASE COUNT         1649 a          568 c          705 g         1585 t
ORIGIN      
        1 taagtggggt taagcgttat aattaattat aaatattgaa taatctaaat ggataagata
       61 atggtattaa aaattatctt aaaaggtgaa ataatctgta tttaatgtag tacagttata
      121 tctttaaagc aaggtggtga aatgatataa ataagatatc taaaagaggg tttgatgata
      181 gtgataaaag atggttttca agtaaagaat taacaagact tataaaggct aaagaagaaa
      241 tagaatggtt attaaatagg gattacaaaa tagacccagt cgttactttt gttggtgata
      301 gatatcagtt ttctataaga caaagagatg cattaaaaag agcagtctgt actgatgaaa
      361 aaaatattat aagacaatca aagaggcttt ctctagataa aatcaaagaa gataccgtaa
      421 atatagatgg ctttaatttg ataatatcca tagaggtagg attatctggt ggtactctag
      481 taataggaag tgatggcaat ataagagatt tagcaggtct taggggaacc tataagatta
      541 tagataaaac agaagaagct ttgaatctga taggtaaatt ttttaataaa tacaatgcac
      601 agaatcttaa attttattta gatgctccag tttctaattc aggaaattta aagtatagga
      661 tattagagca tgccaaaact tggggaattg aaactgaagt agaattagta aaaaatgcag
      721 atgttgtatt ggaaaaatta gatagagtag ttagtagtga tgcagtcata gttgacaagt
      781 gtataagtta ttttaatgtg gctagaggta taattgaaga gtatattaaa gaatgtaata
      841 tcataaattt aaataaatag ataaatatag gtgagaatat ataagtatat gtagcaatat
      901 tcttagtaat acaagtaaag tttgaaaata ggagaacgaa tacatatcga atttcataaa
      961 atgttgtaaa aagacatatg agaggatgga tatgtgtgaa attaactaaa ctaaatatta
     1021 aaaaatatag agcacctata tataaatatg ctttagcaaa tgtaaatttg aggtctgcta
     1081 aatctactaa ttcaagtatc ataacagtaa tacctcaggg agccaaaatg gaagtattgg
     1141 atgaagaaga tgactggatt aaggtgatgt acaattctca agaaggatat gtgtataagg
     1201 atttggtatc agtaagcgaa tatgcttgga gtaacttaaa cttaagagaa gataaatcta
     1261 caacttccaa tataattaca gtaattcctg aaaaatctag ggttgaagtc cttcaagtag
     1321 atggagattg gagtaaggtt gtatatgatg ataaaatagg atatgttttt aattattttt
     1381 tatcaattga tgggaataaa ccaaatgaat tagattataa gtatttttat actgacatga
     1441 ttaaatttgt taacgaaaat aatattaaaa gtactagcga ttatctaatt gtaacagacc
     1501 ttagaaataa atatacttat atttttaaga aagataatgg aggttgggga caactttaca
     1561 agtggcaatg tactataggc aagcctgaga ctccaactat aacaggtata ttttatataa
     1621 gtggaagaaa accttctttt ggaacggatg agtattcagt taagtatgct actagaatta
     1681 aaggtggata ttactatcat tctgtactat atgattcaac aggaagttat ataatagatg
     1741 gcagacttgg tgaagctctt agtcatggat gtataagatt aagtacggaa aatgcaaagt
     1801 ggatatatga taatatacca gatacaacaa cagttataat tcattaagaa aaattttgag
     1861 ttttttattg ggataatgat tcttaaatta ataggattat atattaactt ctcataaaga
     1921 gtactattta aaaagtgtaa taagagagtg ctattaacaa aacaaagata ttttgaggca
     1981 ctctcttata aatatacata attaagatat tttcataaac aattctataa tcttatcttt
     2041 atcaaagaaa acaggattag caccagcaca agcatctttc atagagtttt ctgcaagcaa
     2101 ttcaaaatta ggattatcaa cacctacatc ttttaaagat tttgggattc caacctcttc
     2161 agaaagagct tttatcttat ctattacaaa gtcaacacac tctttatctg atttattatt
     2221 tacatccatt ccaataactt cagctattgg tcggaatttg gcaggggctg atttagcatt
     2281 ttcctcctca actattggaa gtagcatggc attacatact ccatgaggta aatcatataa
     2341 acctcctaat tgatgagcca tagcatgtac atttccaaga cctgcattac taaaggcaat
     2401 tccatttaaa aaacaagcat aacacatttg ttctctagct tctatgtcat taccattttt
     2461 aacagctctt ggaaggtatt taaatatctg ctttatagca tataaggcag ttgaatctgt
     2521 aacatcataa gcaccattag ctactacagc ttctatagca tgagttagtg catccatacc
     2581 agttgctgct gttaaattag atggtttgct aatcattaat tctgggtcat ttactgtcat
     2641 tgtggctagt gaattggtat caaccattat catctttaca tgtctgtctt catcagttat
     2701 aacatagttt attgtaactt cagcagatgt accagcagta gtagttatag ctatgatagg
     2761 aagacatttt ttagaagttt tgttgatacc ttcatagtct ctaatatctc ctccattagt
     2821 ggctaatata gagatagctt taccacagtc ttgtggagaa ccacctccta tagttataac
     2881 gaagtcgcaa ttttctgatt ttaatatttt aagtccttta ttcacattag ttacagttgg
     2941 attttgttta acttcatcat aaacaacaaa gtctatatct atctgagata aaatatctgt
     3001 tacttttttt acagtaccat ttgaaactag aaatttatca ctaacaataa atgctttttt
     3061 agttcctaat gatttaattg aatctttagc ttcattaaga catcctttcc caagtaaatt
     3121 tattggtggt acataataaa tgctcataac taaaatctcc ttttattttc attgattttt
     3181 atgtaaaaat atatatctaa caattttcac taatttactc ataatatatc gtatcttaga
     3241 ttgagatagg gttgctggtt cttatataag aacttaattc gtaattacga ttatgattag
     3301 aatttaacat agtattttta tcatgtcaat aaaaatataa aaaatattat aaaaaaaaca
     3361 aagttactgt agttaaatca gcaactttgt ttttatagta taaatcaaat aaatacatta
     3421 gtttaaaaca aaatttttaa ttattatgtt ttgatatctt ccatatacag aatattaatc
     3481 taatttatta caatgtcttc attacgttct tccatcattg attgaatatt tttctgagca
     3541 tttaagagta atttttttat cagttctatt ttttcgtcat tagctctaaa taaaggcagt
     3601 gttacactaa cagcagctct tatacatcca ttttttctta ttggtacggc aagacatttt
     3661 aggtgttcca ttgattcctc acactctgaa gctatttgtg ttctttgaac ttcaagaagc
     3721 tggtcgtata aaacatcaaa atctgtaatt gatttttctg taaaaccttt tagaccatct
     3781 ggatataagc ttattagttt ttccttgtca aaatcacata aaagagcttt tccaagacct
     3841 gtacaattag caggtatttt ttttccaaca taagaaataa gtctaatcgg ttctggagaa
     3901 tctacttttc caatataaag aacttcatta ttgtcaagta taccaagctg acaagtttca
     3961 gaacaggaat taactatatt agtcatttcg tcaattatat agttaaaaat agttcttttt
     4021 tcaaaaaatg aagaccctaa tgtgtaagaa cgtatcccta tagaatattt ggatgtgttt
     4081 ttatcaatag taatgtattt tctagcatta agtgtatgaa ctataggaaa gatgctactt
     4141 tttggagcat tgataacatc tgctatttct gtaagagtat atccattttc ggattctgct
     4201 aatgcttcta aaatatctaa tatcctagct gttgggcgat gattatctga agtcataaat
     4261 ttccccctct ttttattaat ttaaagtatt agaatatgtt gattttagca cactatacga
     4321 tattttacaa tgtagaaaat aaatatgtaa atttatatat aaataagctc ttgatttaga
     4381 tacagataga tattttttta gactgtacat taaggaataa ctaatttcca aaatatacct
     4441 aaagaaagta agtgatattt agttatataa tttaagtaaa tatatgcaat agttattttt
     4501 atatgta
//
LOCUS       DAEOSP010000245         4472 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.249, whole genome shotgun sequence.
ACCESSION   DAEOSP010000245 DAEOSP010000000
VERSION     DAEOSP010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.249"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     misc_binding    62..296
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            346..1365
                     /gene="pheS"
                     /locus_tag="KRH54_003299"
     CDS             346..1365
                     /gene="pheS"
                     /locus_tag="KRH54_003299"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBG8580929.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            1382..3775
                     /locus_tag="KRH54_003300"
     CDS             1382..3775
                     /locus_tag="KRH54_003300"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBG8580930.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMSGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            3800..4390
                     /gene="zapA"
                     /locus_tag="KRH54_003301"
     CDS             3800..4390
                     /gene="zapA"
                     /locus_tag="KRH54_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBG8580931.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
BASE COUNT         1763 a          520 c          919 g         1270 t
ORIGIN      
        1 taaaaatagt cacataattt gaatggaaat gatataatac taaaataaac aatataatat
       61 tgtaaatgca atgaaagagg aaagtatttt gattaaactt agtaaagaga taaacaccta
      121 ggctgggagt gttttctaag aggtcatgag aagttccctc tggagtaaca gagctgaaat
      181 tttacagtag gctttgacgt caaaaacgcg ttaagtttgt tagaggtggt ttgatgattt
      241 ttaaattgtt aaactactag ggtggtaccg cgaaactata gcctttcgtc cctagacagg
      301 gattgagggg ctttttttat acaaaaaaac gaaagggtga tatgtgtgca agaaaaatta
      361 cttgctttac gtgaagcagc tttggctgaa ataaaagaag cacaaagcat agaaagtgta
      421 gaaagtttaa gagttaagta cttaggaaaa aaaggtgaga taactgccat acttaaagaa
      481 atgggtaaat tatctgctga agaaagacca gtagttggta aggttgccaa tgaggtaaga
      541 gaaaacattg aacttagcat aaattctaaa aaagaagaaa taaatgctat tgaaaaagaa
      601 agaaaattaa aagaggaagt gatagatgtt actcaaccag gaaaagtttt aaaggttggg
      661 aagaagcatc caataactca aattatagat gaagtaacag atatatttat cggaatggga
      721 ttctctatag cagaagggcc agaagttgag actgttgaaa acaactttga cgcattaaac
      781 gctcctaaag accatccatc aagagatatg agcgatacat tctatatcaa tgatggggta
      841 ttacttagaa ctcaaacatc tccagttcaa gtaagaacta tgagaagtca agagttacca
      901 ataaaagtaa ttgcaccagg tagatgtttt aggtcagact cgccagatgc tacacactca
      961 ccaatgttcc atcagataga agggcttgtt gttggaaaag atgttactat ggcagaattt
     1021 aaaggaacta tggatatctt cgttgaaaaa ttgtttggtt ctgatatcaa aactaagttt
     1081 agacctcaca acttcccatt tacagaacca agtgcagagg ttgatgttac ttgtttcaaa
     1141 tgtggtggta aaggttgccc aatgtgtaaa tatgaaggtt ggatagaaat attaggttca
     1201 ggtatggttc atccaaatgt gcttagaaat tgtggaatag acccagaagt ttacagtgga
     1261 tttgcatttg gagttggggt tgaaagactt gcaatgctta aatatgaaat agatgatatt
     1321 agattattat tcgaaaatga tatgagattc ttaaatcaat tttaattagg agggtatgta
     1381 gatgttagta tctttaaaat ggcttagaga ctatgttgat atagacatgg atgtaaaaga
     1441 gttcgctgat aaaatgacaa tgtcaggaac taaagttgaa acaatagatt attatggtga
     1501 agaaatagaa aatatattgg ttggaaagat tttagaaata aaacaacatc caaatgctga
     1561 taagttggtt gtaaccaaag tagatattgg agataaagtt gttcaaatag ttacaggagc
     1621 tacaaatata tcagaaggag attatattcc agtagctgta aatggttcta agttacctgg
     1681 aggagttgaa atcaaacaga ctgatttcag aggtgaatta tcagatggta tgatgtgttc
     1741 agcagctgaa ctaggtatag atgaacatta cattgaggag tataaaagag gtggtatata
     1801 tattttagac cacgaagatt cttatgaatt aggaaaagat ataaaagatg ttttaggatt
     1861 aaaagatgct ttaatagatt ttgaattaac ttcaaacaga cctgattgta aatgcatgat
     1921 gggtatagct agagaagcag ctgcaactat aggaacaaaa gtaaaatatc ctgaaatcga
     1981 agtaaaagaa agtgacgaag agatagattt caaagttgag atagataatc cagatttatg
     2041 tagaagatat gttgctagaa tggttacaga tgtaaaaata gaaccttctc catattggat
     2101 gcaaagaaga cttacagaag caggagtaag acctataagt aacatagtcg atataacaaa
     2161 cttcgtaatg ttagagcttg gtcaaccact tcatgctttt gatataaatc aagtagagac
     2221 tggaagaata gtagtaagaa atgctaaaga tggagagaaa cttgtaacat tagatgatgt
     2281 tgagagaaca ttagacaaag atatgctagt tataacaaat ggagaaaaat cacttggttt
     2341 agctggtgta atgggtggtg ctaactcaga aataacttct aatacgaaga ctgtactttt
     2401 tgaaagtgcc aatttcaaac cagaaaacat aagaatgaca gctaaaaaag ttggtattag
     2461 gtcagaagca tcttcaagaa atgaaaaaga cttagaccct aatcttgcag agatagcagc
     2521 aaatagagct gcacagcttg ttgaaatgtt aggagcagga aaagttttaa aaggtgttgt
     2581 agatgtatat ccaaataaac cagaacctaa aaaattggta gtaaatcctc aaagaattaa
     2641 ccatctatta ggtgtagatg taccaatgga gcagtttgta ggaattttag aatcattaga
     2701 gtttaaatgt aatttggtag ctaatgataa attagaaata gatgtaccaa gctttagaac
     2761 agatatggaa caagaagctg atgtatggga agaaatagct agaatttatg gatttgagaa
     2821 tataccttct gtacaattag aaggaaatac gacagcagga attaaaactt caaaacaaaa
     2881 attcatggat gctttaaaag ataattcaac tgctgtagga ttaaatgaaa tattaacata
     2941 ttcatttgta agccctaagg gagtagataa gataagagta ccagaaggta atgcaaagag
     3001 aaattttgtt aaattactta acccattagg agaagaaact tctgtaatga gaactacttt
     3061 aataccaaat atgttagatg ttttatcaac taatgtatct cataaaatag aagaagtatc
     3121 tgcatttgag tgtggacata tattcatccc acaagattca gaattaccta aggaagaaaa
     3181 tagaatgtgt gtaggtatgt atggtaaaga tgttgacttc tttgcactaa aaggaactat
     3241 agaaactata cttgtaaatg tcggatttaa aggttatgaa attgaacctc aagataataa
     3301 tactaccttc caccctggta gatgtgctaa aatagtgtat aataataaat atgtaggtac
     3361 attaggtgaa ttgcatccag atgttataga aaactacaat ctaggtcaaa gagtttatgt
     3421 cgctgaaata gatattgact ttgtatttga taattctgat agaacaaaga attatgtgcc
     3481 attaccaaaa tatccatcta catcaagaga tatagctctt attgttaaag atgatgtatt
     3541 tgtaaaacaa atagaagata taataaagga aaatggtcaa ggattagtag aaagctacaa
     3601 gttatttgat gtttacaaag gttctcaaat agaagctgga tacaagtcaa ttgcatattc
     3661 aataacttat agaagtaaag ataaaactct aacagatgaa gatgtagcta aggtgcatga
     3721 taagatatta agtgaattaa gcgaaaaatt aaatgctaac ttaagatcaa attaacaact
     3781 aatgtcgggg gaggttagga tgaacaaagt aatggttaaa atccatggcg ctgaatatcc
     3841 aatggttgga gacaaatcag agaagtttat gattagtatt gctgattttg tagacaaaga
     3901 aatggataaa ataacacgtc aaaacccgaa attgagctta tcagttgctg cgatattaac
     3961 tgcattaaat atatcagacc ttttatttga atgttcagat gagaatgaga agttgattaa
     4021 agctaatgaa gaattgagta agaaggttgg agcatctaat gaagagcttc aattagaaat
     4081 caagtcttta aagcttacga tagcagaaaa agaagcagaa aatcgtgaag ctgaaactaa
     4141 gatgaaagag ttaattgaga ttatagaaaa taaaaagcaa gaaatattcg aacttagtaa
     4201 tacgacagaa ggttctagag cagagttaga tgcttataaa aacaagattg aggagttaag
     4261 tactcaactt gaagaagcaa atgaaagagc tactatagct gaaaacttag cttctgagtt
     4321 tcaaaacaaa gcttatgatt tacagttaaa atgtacaggg ttaaataatg atgcaaaaaa
     4381 tgtggaataa actaaaagat ttgctgtctc ttgttttttg agacagcttc ttttaatgta
     4441 taaatataaa tgctatataa atatagataa tg
//
LOCUS       DAEOSP010000246         4453 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.250, whole genome shotgun sequence.
ACCESSION   DAEOSP010000246 DAEOSP010000000
VERSION     DAEOSP010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.250"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(296..457)
                     /locus_tag="KRH54_003302"
     CDS             complement(296..457)
                     /locus_tag="KRH54_003302"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580932.1"
                     /translation="MKNQANGLKNELIIGKNQAYRLENELIIGKNQAYRLENELIIGK
                     NQAYRLKNK"
     gene            complement(672..890)
                     /locus_tag="KRH54_003303"
     CDS             complement(672..890)
                     /locus_tag="KRH54_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8580933.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(891..1298)
                     /locus_tag="KRH54_003304"
     CDS             complement(891..1298)
                     /locus_tag="KRH54_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580934.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(1384..1872)
                     /locus_tag="KRH54_003305"
     CDS             complement(1384..1872)
                     /locus_tag="KRH54_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBG8580935.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            complement(2077..3174)
                     /locus_tag="KRH54_003306"
     CDS             complement(2077..3174)
                     /locus_tag="KRH54_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG8580936.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIAKGKREEKFNIII
                     KLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            complement(3547..3717)
                     /locus_tag="KRH54_003307"
     CDS             complement(3547..3717)
                     /locus_tag="KRH54_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580937.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            complement(3875..4318)
                     /locus_tag="KRH54_003308"
     CDS             complement(3875..4318)
                     /locus_tag="KRH54_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8580938.1"
                     /translation="MIEIKTIEINKKSQIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT         1493 a          711 c          397 g         1852 t
ORIGIN      
        1 tttggaagta atttcatatt tatttaactt aatttttttg tgataaaata attctaattt
       61 tcatatagat attattataa attttctagt ttatggagtc attcattcta taattcacaa
      121 atatgtattt tttaatctat taaagtattc attttctggc ttataagcat ggacttctta
      181 tctataagct tgttttttca tctaataagt tatttattct ttaatctatg agcttgattt
      241 ttcatctata agtcatttat tttttaatct atgagtttag ttcttcatct ataaatcatt
      301 tattttttaa tctataagct tggtttttac ctataattaa ttcattctct aatctataag
      361 cttggttttt acctataatt aattcattct ctaatctata agcttggttt ttacctataa
      421 ttaattcatt ctttaatcca ttagcttggt ttttcatcta taagtcattt attctctaat
      481 ctataagctt ggtttttacc tataaatcat ttattctcta atctataagc tttctttttc
      541 atctataaat cattcattct ttaatccatt agcttggttt ttcatctata agtcatttat
      601 tctctaatct ataagcttgg ttttttacct ataattaatt cattctctaa tgcataagta
      661 atacatgaaa tttaaatatt attttcttca ataaattcaa ataactcttc tacagttgta
      721 tcaaatacct tagcaatatc cattgccagt tttaaagaag gattgtagcg accattttct
      781 aaatgtacaa tagtttcacg tcttactcct actgactttg caagttcacc ttgtgtcata
      841 aggttctttt ctctatagga tttaattttt gtctttagta atactgacac ctaattgtat
      901 atcccctttc tgtcaaaata tataaataga attaacttca acaaggattg aataaacaaa
      961 atgactaata aagacattcc tatatttaaa tttgacacat ttatgtccaa agttacaaga
     1021 aatatagata ctatagctac agaaaaaaac aatactttta ttgcaatatc attaactctt
     1081 gatagtatac attttgatgc ctcatcaatt aaatcatcat actttttatt atagacccac
     1141 tgagctccca tagctaatac ccaaataatt ataggaagat attcaatact acttccttta
     1201 aaaaaatact tatttactaa tgtagctcca atacaataag aaattcccaa tatttctgaa
     1261 ataagtgttg atttaatacc agtttgttgt ttagacatat aaaatccccc ttaaaaagtt
     1321 atgtatttat aacacctctt gttataaata tataactttt ttgaataaat ttcaataaaa
     1381 atattatttt gcaacaaatt cttttattaa ttttacatta gatagaggca ctttagatgt
     1441 cactccttgc aatgtcaaat cttctatatt aacttcattt aattttgaac acagttctaa
     1501 gtcaaatatt ctttcccaac ttaccttctt ttcaatttgt tcatcttcat aaagctctac
     1561 agtaatatcc attaaaggta aatgccataa atcaaattct gataataaca cattgtcttc
     1621 acttatttct atttcaagta aaacaccttt ttttcctttt tctaaataac cactacatct
     1681 taaatcaggt ctcttcgtcc aaacccatat catactatca tcaaattcat atattctatt
     1741 tgtcatttga ctatacatcc atttgtatgg aatatcaaag tcttctaata taaaagaata
     1801 atcatttgat agatatttat tgtttttatt gttattccaa aattctaaag tctgtatagt
     1861 ataaaatttc atatataaat caaccccttt gtttttctat aagaaggaga tggttcaaac
     1921 ttttattttg aattatctcc tttatttata cacctctttg tacctattat ttatatatct
     1981 ctttatatct accatattta aataacaaag ttttatatta atttaaattt aaaagaggaa
     2041 gctttgtatt tatctagtgc catctcaatc atagtattag ttttgattat agactttttc
     2101 aacctctgat ttattcaaat catctataat atcgcatatt tcactcaaat tgtatccttt
     2161 attttttaat tttatgatga tattaaattt ctcttctctt ttcccttttg caataccttt
     2221 ttcgatacct tcttgaaaaa tttcttcttt aaaatttcta cttttattca taatatacgc
     2281 acctctcttt aaatttaata aatagtcctc attactacct aattctttta atctatcaag
     2341 tgctctttta ataacataat ccgttttaga tagtattcta atatctatat gagaaggatt
     2401 atcaataagt ttaccccata ttagaactct gtttgctata caactggatt tacgaatttt
     2461 tggaagttct ataaaatata atgcagtttt actagaaaaa atatctggat gcccgtccat
     2521 tgttaatctc aggcaattta cataatcttc tatgtgatta tattttacat gatttagtat
     2581 atgtattcca acagtttttc caatttcttt atattcttta ccttcttcta actttctacc
     2641 atgtacacga ctaagataat attcagctct ttccaaataa tcctcttcaa gagctctttg
     2701 catctcaata tttatatggt tacgttcttc atcttcagcc aaaatatcaa gtctaggtct
     2761 tttctcacca atgtaattaa ttgttgggtc ggtttcaacc ttaattattc tttttatttg
     2821 agtgaactgt ttgatattcc tattggcata tggagcatct ttgaggatat catttataaa
     2881 atttgcaagt aaatcgtaat cattatttaa agtaaacaat gatttaaata cctcatcata
     2941 ctcaggctta gcatacccat cttcagttac aacttctcca acattttcac tagtcaatat
     3001 ttcttttgga attaaatcct tgattttatc taatttttca ttagaaaaat cacctctact
     3061 aagtatgtca tttaattttt tggtccattc tctgtccttt tcttcaatta tagttcgttt
     3121 atactctttt gatgttttaa taatcttaga tttttcttct aatttttctt tcatatcaac
     3181 acctcttttt tattattacc catttcttca taccaaagtc tgaacttcta agtaaaattt
     3241 gcattattga aataagaacc ttataaactt atatatcatt actaaagtta agactttaaa
     3301 gagaagcact tagattacaa tagattttag atagctttct ttttaaaact attgttgata
     3361 aatgaatgaa tataagggta tgtattaaaa tagagatact catctttttc attgtttaaa
     3421 taatgaaaat atattttatt taatacaaaa aagagtgcct tataaactaa aagttcactt
     3481 cgagacactc ccttacattt tgtttcttaa actttacaat attacatact tatttattaa
     3541 tttaatctat gcatacttaa cccaataata aagtacatat atccaactca ttaagccatg
     3601 aaaaatagcc catggaatag atttccatgc agtataagaa ataatcattg ctaaacaaga
     3661 accaaaagaa ataccctttt taacagttga ttttatagta ttattatttg aatccataat
     3721 taatcctcca aaattcaaaa taaacaagtt attatatatc ttatttgaat ataatacaat
     3781 ataacagctt ctaaataaat tatactctta ttatacataa tttataaatt ataattccga
     3841 taattatatg aattaattaa ttatattaac ctatctaaaa gttatatgat agatacaaca
     3901 tgtcaacaca ttgtaattct ccttcaaaga ttggttctgg ataattatct acaaaaaagt
     3961 ttttaatttt atgtgaataa acaaatccaa acttctcata aaaaggaact ccgctttcag
     4021 atgtaccaac aaccattgat gaatattttt tactataaac atctaaaaga tgataaatca
     4081 tctttgaggc ataacccata ttttgaaaat gctcatatgt agcaatgttt tttaattcac
     4141 atatttcttt ggaaatttca tttacaactg ctatacaagt aagttcttta ttatcataca
     4201 atgcgtacat agttccttta ttgagatatt tatcaatcat ttcttcacat gggtcggcta
     4261 acattaaaag gtcaatttga ctttttttat ttatttctat agtttttatt tctatcataa
     4321 gttctccacc ctcactttat tcaaacctta atttaacaaa cttcttacag tacttattta
     4381 gttcattttg atatatattt ttacttttta aaatatagtg ataattccct agaatattaa
     4441 tactaataat aaa
//
LOCUS       DAEOSP010000247         4420 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.251, whole genome shotgun sequence.
ACCESSION   DAEOSP010000247 DAEOSP010000000
VERSION     DAEOSP010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.251"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            128..1267
                     /locus_tag="KRH54_003309"
     CDS             128..1267
                     /locus_tag="KRH54_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBG8580939.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSLNSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HIGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKEFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFSNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1293..1481
                     /locus_tag="KRH54_003310"
     CDS             1293..1481
                     /locus_tag="KRH54_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580940.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
     gene            2265..4049
                     /locus_tag="KRH54_003311"
                     /pseudo
     CDS             2265..4049
                     /locus_tag="KRH54_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
BASE COUNT         1907 a          489 c          714 g         1310 t
ORIGIN      
        1 tgcattattt attaaaattt attatgcttt aacgctaaat tgagtttgat aattataaaa
       61 caaataatca aaaatagtgt aaaaattaat actttttgtt aatattaata gataaagata
      121 atctaaggtg gtgattaaac tgacaataga agaatacaaa aacaaatact ttactggaaa
      181 taaaatcgta ctttctctaa atagttttga taataaagaa ctatactatc atgaaactga
      241 aaatatatta aatttaataa atgaaataga taaattaaca tcaagtaaga tatttaaaac
      301 aatagaagat aaaaatgatt tggcactaga gtcttattat tctagtgtaa tagaaggtgc
      361 tttttcaact agaaagattg caaaatctat aattagagga aagatgaaac ctagtaataa
      421 aagtgaatat atgatttaca ataatcatag agccttagaa tatggattag acaacttaga
      481 taaattatat agtcacaaat ttatatatga tttgcatcat atattgggcg aaaactgctt
      541 ggattcagaa gaatatgagt atagaactga aaaagtatat gtatgtgatt caaaaggtga
      601 gattatacat atagggcttg aacctttgaa gatatatgac tttatgagta aattaataga
      661 ttttatggaa aatagcaaag taagtaattt aattaaaagt gctataattc atttttactt
      721 tgtatatgtt catcctttta gtgatggtaa tggaagaaca tcaagagcct tatcatatct
      781 atatttgatt gataaaggat atgatacttt caaggagttt tcaatatcat atatgatatc
      841 taaaaacaga actaagtatt ataaggctat acttgatgta gaaaacaaag gaaataattt
      901 aactgtattt atagagttta tgctcaagtc tataatacaa tctattaatg agatgcgtaa
      961 tatgcatgat agaaaatctt tagaaagtat attaaaagaa gaattgtttg aaaatgacat
     1021 aactctatca gcaacagaag aacatatatt aaaatatatt tgcaataaag ataactattc
     1081 tatgacatta gaaaattata taaaaaagaa taaaagtaga tacttaaagg ctggaataaa
     1141 ggaaattgaa ttagttgacc agttgatgga agtgtttagt aatcttgaag agatagagat
     1201 tttgtctaaa gaaaaaaata tatataaagt taatgataag tacttaaaaa tgcttgatat
     1261 agactaattt agataatctg gagagggaat atatgaataa taagaaaaag gcatttttga
     1321 tttctggggc tataaacgta atttcattaa ttattgtttt ttcattgttt cattataata
     1381 ttatagaaaa tagtacacaa aatatgataa taactgtaat cttaattttt atttttgaaa
     1441 tgataaaaat aaaaatcata gagaaatact ataaactata aacaagaaat atcttttcta
     1501 agagcatatt gtacaatatg ctcttaattt agttatatag atttaatata aataatgtat
     1561 tttttgtaga tgatttatgt aagatttatg accaatttgt gtatgattta tgttctttaa
     1621 tcatgacaat attatattgt aaaaaataaa tttaataaaa aatgacttgg aatttatatt
     1681 ctaggtcttt tttatataaa aatttaataa gtttcaaatg ttggtaactt tgacagtaga
     1741 aaacgtctat tgagataacg aggattctaa tttaggggga gatatattag cacttaaagt
     1801 agtatatgca acagatataa tctttataaa agtaattcta attaaaatgt aaatataaag
     1861 aataattaaa aaaatatcgc aattatttgt ttttcatatg gataaaattg gttatttaat
     1921 atataattac aagtataaat aataaaattt atttgtaaaa agagtagcac ttttaattct
     1981 aaaggctact ttttttatac aaaaccctat tttttataac aattcattaa cataaaaatc
     2041 aaacttacct taaaatataa atttacaaat ttttcaatca ataagaaaat ttctttattc
     2101 cttacataat gactgcaatg taacaaaata attacaaatt aattatgaaa tcattatagt
     2161 tagaaacctc tttaaaatta gatattaagt atcctttaga attagaacgg gaacaaagtt
     2221 aaaaaattaa gtaatatcaa atggacaagg gggaagaaaa taaaatgaaa ataccaaaga
     2281 agattgctgc aatgcttaca gttacaatga tagctggaag ttcaacagca ggcatagcta
     2341 gtgcacaaac tgtggcaaca aatttaacag ggcaagaaag atatgaaact gctgttaaaa
     2401 taagtcaaga tggatggaaa aatgctgatg aggtagtaat cgtaaacgac tcatctatag
     2461 cagatgctct atctgcaaca ccatttgcaa aagcaaaaaa tgctccaata cttttaacaa
     2521 gtaaagataa attaaatgat aagacgaaag cagaaataca aagattaaaa gctaaaaaag
     2581 tatacttaat aggtggaact tctgtattaa gtacaaatat agaaaaagaa ataaaaaatt
     2641 taaaaatatc ttttgagaga atctctggag ctgagagata tcaaacatcc ttagaattag
     2701 caaagaaatt agatgctata agtgatgtta agaaaatagc tgtagttaat ggtgaaaaag
     2761 gacttgcaga tgctgtaagt gtaggagcac cagctgcaca aaacaatatg ccagtaatac
     2821 ttgctgattc taaaaatgga acagcagttg cagacaaatt tataaaagat gctggaataa
     2881 ctcaatctta tgtagtaggt ggagagagtt caatttctga agctgttaaa aataaactac
     2941 caaatagtac aagactaggt ggaacagata gaaatgatac aaatgcaaaa gtaataaaag
     3001 aattttataa aaagacagat ttaaaaaatg cttatgtaac taaagatggt atgaataaac
     3061 aagaccaact tatagatgca ctagcagtag gtgtattagg tgctaaaaat caatcaccag
     3121 tagttttagt tggaaagaac ttatcagctt cacaaaaatc tctagttaac tctaagagtt
     3181 ttgataagat aactaaagtt ggtgggaatg gtaatgagac agcatttaat gaaatgaagt
     3241 cattacaaga agtaaaaaca gttgaagcta aaacaatttc tgaattaaaa tcagctatag
     3301 ataaagctac tgctaatgat gttataaact ttaagccaac tagtgaggtt aaagaagcat
     3361 ttacaataca aactgataaa gcagtaacag taaacttaaa tggtacatat acaaagactg
     3421 taactatcaa catgcctaat ggagatgtaa ataactatgc aaaagtggat gatttagtta
     3481 tagatgatgt aaaagatgga acttttgtta actatggaaa aataactaac ttaaaggtta
     3541 atgataaaaa tggagccaag atagaaaata attctaaagg tgagattggt agtttaacag
     3601 tagcatctgg agcaagtcaa gtaaaagtga ctaatggtgg taagataact acagttacaa
     3661 ataattctaa agggactact atagataata aaggtacaat aagtagtgtt aagggagata
     3721 attctccaac aataagtgga aatagtccaa gttctaattc atcaggagga agttcatcaa
     3781 gtggaggttc ttcacatgga ggaggctcat cttcaggagg aagttcttct aatcaaacat
     3841 cagtaaataa tgaagcagca aaaataacaa gcgtgcctac accagcaaaa gatgcaacta
     3901 gattaactat gccatcagta tcaagtggat atactatagc aataaaaaca tcaagtaatg
     3961 agagtgttat aaaaaaagat ggaacaataa tacctccaaa tacagcaaca acagtaaaat
     4021 tagtatttac ggtaacacat acatcaagtg gtaaaacagc agatacaaaa gaactatctg
     4081 taacagtacc agctaaatca acagatgaag aattacaagc agcattagac aatgaagtgg
     4141 caaaaataac aagcgttcct gcaccagcaa aagatgcaac taaattaact atgccatcag
     4201 tatcaagtgg atatactata gcaataaaaa catcaagcaa taagagtgtt ataaaggaag
     4261 atggaacaat aatacctcca aatacagagg aaacagtaac attggtattt acagtaacac
     4321 aagaatcaag tggtaaaaca gcagatacag gagaaataga tgtagtagta ccagccaaat
     4381 caacagatgg ggaaatacaa gcagaagtac aagctgaggc
//
LOCUS       DAEOSP010000248         4408 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.252, whole genome shotgun sequence.
ACCESSION   DAEOSP010000248 DAEOSP010000000
VERSION     DAEOSP010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.252"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(44..970)
                     /locus_tag="KRH54_003312"
     CDS             complement(44..970)
                     /locus_tag="KRH54_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG8580941.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            1160..3031
                     /locus_tag="KRH54_003313"
     CDS             1160..3031
                     /locus_tag="KRH54_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBG8580942.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(3123..4058)
                     /locus_tag="KRH54_003314"
     CDS             complement(3123..4058)
                     /locus_tag="KRH54_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBG8580943.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
BASE COUNT         1735 a          484 c          694 g         1495 t
ORIGIN      
        1 taatttattg aatatgttat tatataatac ataaaaaaat aaatcaagat aagagtaaaa
       61 tacttataaa aggtatagat ataactgaaa atagtgttgt aaaaaacaca cattgtgaag
      121 ctagtgttat attggagtca tattgattag ccattatagc agtatttgca gcagcaggca
      181 ttgaagaaat tactactggt atagctaaaa tcatcttatc atttatccat ccttttagaa
      241 taaaatacac aatcacagga agtaccaata atcttataaa agtgactata tataattttt
      301 tgttgacaaa acaatcgaga gctgaagaat tagcaagtaa gcttccaatg ataatcatgg
      361 atattggtgt agttatactt cccaacatct caagtggctc gtttataaat tggggtaacc
      421 taagtcctgt tacaaataaa aaaagtccaa taacaatagc tattgtagca gggcttataa
      481 gtgattttat tgaaaaacct ctacttgtac tgcctttaga taaaagataa attcctaaag
      541 tgaaagaaaa taagttaaaa ggcaaattga atattgcagt atagaatatt gcttcatgac
      601 ctaaaactgc ttcaattaca ggataaccca taaaagcaac atttgaaaaa acaactatat
      661 actggtatat tcccaaatct tttttatcat tacattttaa aatgtatttc aataaaaatg
      721 caattataat tgaaactata tacataatca aagatgtaaa taataacaat agtatttttt
      781 gtatcatttt actatcaaag ttaatttgca tggagctaat tatcattgat ggtaaaaaaa
      841 ctgtcattgt caaagttgaa agcttagatg tgcagtggtc atctaataag ttgaattttc
      901 tgacaaaata tccaactaaa ataattataa ataaaactgc aacttggata aaaatattgc
      961 tgaaattcat aaaatgtccc ccctatatat acattatata ccaattacat aataaaatct
     1021 aatgattata aaatttaaaa tttaatgtta caatatagtg acaaaaggta aaaattcaat
     1081 attatagcat taattgtaga ttataatgta taataatgtt tgagagaata tattttataa
     1141 aatgggtagg tagcgaataa tgaaaaatac aaaattttct ataaaaaaaa taaaaaatat
     1201 atctaatgac ccgaaagtcg tacatactaa gggtgccctc cttgaaaaga tgggtaagat
     1261 ggaaagcgca atagagttgt ataagagtgc tgcattggat aattacgtaa aatctcaata
     1321 tactttagga aatatatatg aaagtaaaaa acaatttaaa gaagcagaaa agtggtattc
     1381 aatggcatat aaaagtggaa gtgaagatgc tgcttttgat ttaggtaata tgtactataa
     1441 gttagatggc tatgaatatg ctatatattg gtatgaaaag atagcaaatt taggttattt
     1501 acaagcacaa aataatttag gtgtatgcca ttttaaaatg aaagattttg taaggtgtga
     1561 aaaatggtta aaaacagcag cagacaagaa tttaggtaaa gcttgtttta atttaggggt
     1621 attatatact tttttggaga aagatgatga agcatatgaa tactataaaa aaggttctgt
     1681 gcttttagat gatgatgcaa aatataattt agcaatatta aatggaaaaa agaaagataa
     1741 taaatcaaca gtaagtttat ataaacagtt gtacaagtca ggtcatatga aaggttgctt
     1801 taacttgggt atgattatgg aaataaatga taatttagaa gatgcagaga agtattataa
     1861 aaagagtgct gatagagatg atgtgaagtc tgagtatagg cttgcatatg tatatgatag
     1921 aaaagaagag ctgggagatg caatttatta ttatgagaaa gcaatagaaa aaaatcatac
     1981 actttctaag tatagacttg ctaatttata taatagagaa aatgagatag agaaggcaaa
     2041 aacttattat aaaatggctg ctgaagatga tataacagaa gcaaaaaata atctggcagg
     2101 tatatatttt gaagaaaaag attatgaaaa tgctattaaa tattatgagg atgctatagc
     2161 tgttggatgc aaaagttccc ttgaaaatct aggggattta tattatcaga atcaagatat
     2221 agaaaaagcg atttcgtatt attcgaggat tccaaataat gtatcttgtc aaatcaaatt
     2281 aggaaatatc tatgaagatt taaataatat tgaagaagca ataagttggt ataagaaagc
     2341 atctgaaaat ggagatacga ggtctagtta tagattgggg tgtatatatg agagcttagg
     2401 aaatacaaaa aatgctagaa agtattttga aatggcaagt agcaaaaacc atatgaatgc
     2461 tcgtatacat ctaggtagaa tttattttag agaaggtaaa ttggaagagt ctaaaatgat
     2521 gtttgataca cctgccaatg aaaataatgt gtatgctcaa catatggtag gattaattta
     2581 tgatatgttc tataaagatt atgtaaattc taaattttgg tatgaaaagg ctagagcaca
     2641 aggttgtgtt gaatcaatat ataatttagg tcaaatttat ttaaaattaa atgatgacgc
     2701 agaagctgag aaatattata aagaaggtat taaatatgga agtaagaagt gtgagtatat
     2761 gcttgcaggt ctttattata aaaagagcct agatatgtac atttctttag caaatgaaga
     2821 gtacgataat tgtgaagaga tagtagaagg ctttccaaat ttaaatatag actttgatga
     2881 agtattaatt cctccattta agttgcaaga agaggtaata gatgaagaag agtatgtacc
     2941 aatttatata ctaaatataa aagaaagttt agattcaatg ctagaagggc ttgaaactga
     3001 aatgattata gatgaagaac atgatagtta aaaagtatta taagattata tagatttaaa
     3061 tatataaaag ccattaggat aaatgtaaat caattaattc taatggcttt tttattaata
     3121 ttttataata gcttatttct tgacatgaaa aatgtaatca ctatacatat tatagaggta
     3181 ataagaagta ttatccaaaa tccgtttggg ttattagaaa atggcatgtt gatgacattc
     3241 ataccaaaga atcctgatat tattgtaggt attgagaaaa ttagtgtaac tactgttaaa
     3301 atttgcataa cattattctg attattactt ataattgcac tgaaagcatc cataattcta
     3361 ctcaatatat ctccatatat tttagccata tcaagagcct gtctattttc aactataaca
     3421 tcttctaata ggtcttcatc atctggatac ttctttatac cagcagttct aaccattttg
     3481 tttaaaacaa gttcattaga ttttagtgag gtagagaagt ataccaaaga tttttctaat
     3541 tccaataatt gaaccaattc tttgtttttc attgatttgt aaatttctcg ctctattatt
     3601 attgtcattt tatttatttt tcttaagtaa tgcaagtaat aagctgcatt tttgtgaaga
     3661 atttgaagca aaaagcgagt ctttttaaat gtaaaaaaat ctttaatatg accaactata
     3721 aagtcattta aaatcttagt ttgtgcatca caaactgtta caatagcttc ttcagttagt
     3781 ataataccta agggtattgt agtataatga gaagaattag agtcactttc gtctactggt
     3841 atatctataa gtataagagt atgattgtct tcaacatcta ttcttgagcg ttcttcttca
     3901 tctagtgcag attctatgct ttctacatct atatttaata aattagaaat ttcgttgatt
     3961 tcactatgat taggttcaac aagatttatc caacagccat cttcaaaaaa actaagtttc
     4021 tctaattttg aatctatagt tttgtaatat cttatcatgg caacacatcc tttattcagt
     4081 tttatataaa tctcatcatt taatagtatc tattaattaa ctaatttata taactgaata
     4141 tatgtattag ttttgatttt agatattatt aagtgatgaa aatgtaaaaa attctctccg
     4201 caacggacta gtatccattg cccatcactc tccttattta ttattttata tatagctatt
     4261 tatgaaatgc gtattttaag cactctttaa tataatacct tttccaggta tataaatcaa
     4321 gttttgattt gattaaataa tatttttacc ataatttaca cataaagtat ataagttata
     4381 tttaatttga gtaaagaata tatatatt
//
LOCUS       DAEOSP010000249         4391 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.253, whole genome shotgun sequence.
ACCESSION   DAEOSP010000249 DAEOSP010000000
VERSION     DAEOSP010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.253"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(213..1256)
                     /locus_tag="KRH54_003315"
     CDS             complement(213..1256)
                     /locus_tag="KRH54_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBG8580944.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
     gene            complement(1277..1723)
                     /locus_tag="KRH54_003316"
     CDS             complement(1277..1723)
                     /locus_tag="KRH54_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBG8580945.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            complement(1897..3477)
                     /locus_tag="KRH54_003317"
     CDS             complement(1897..3477)
                     /locus_tag="KRH54_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBG8580946.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTIN
                     ATDLISNISLTMKELLDI"
     gene            4276..>4391
                     /locus_tag="KRH54_003318"
     CDS             4276..>4391
                     /locus_tag="KRH54_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8580947.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGS"
BASE COUNT         1420 a          767 c          424 g         1780 t
ORIGIN      
        1 tatataatat tatataacac tttttacatt agtaaaggat aaattgtata aagattctgt
       61 taatttattt ttataatttc atctttctaa taaaaacaaa gaagaagact taaaaattta
      121 taagtcctct tcttttcaac gtgcattttt gtgctatatt taattaattc gacctttaat
      181 ttccatatcc ttcctatgat aacaaaactc ttttattttt ttaatgattc attatcattt
      241 aaaacttctt ttatatcaag tacttctttg ttatcatcac tcataacttc ctttattcct
      301 ttgaatgttc ctaggaaatt aactgcttct gaaggtaaaa ccagttttgt tgacttacct
      361 tcagccattt tttcaagagc ttccatagat tttaatgcta gaatattatt gtctatatca
      421 gcctctttca ttgctgaaaa aactaatttt aaaccttctg ctgttgccat ttgagttctt
      481 tttatcattt cagcttcacc ttgtgcctta gcaatagctg tctgtcttat agcttcagct
      541 tcaccttctg ccactaatat agcagattct ttttcacctt ctgctacacg taccatagct
      601 tcttttttgg cttctgctgt taatatagca gattgttttt caccttcagc ttgaagtatt
      661 gcagctgact tattaccttc tgcttgaagt attgcctctc ttctttctct ttctgctctc
      721 atttgttttt ccattgcaac ttgaatatcc tgaggaggca ttatattttt taactctact
      781 ctatttactt ttatacccca tttatctgtt gcttcatcaa gaatagttct catttttaca
      841 tttattatat ctcttgatgt taatgtttca tctaaatcaa gttcaccaat tatatttctt
      901 agtgttgtag ctgttaaatt ttcaatagca gctattggat tagctatctc aaacacatat
      961 ctaactgggt ctgtaacttt ataatataca actgtatcaa tctgcatagt tacattatct
     1021 ttagttataa ctggttgagg tggaaaatct attacaattt ctctaaggtc aattacatat
     1081 gccattttat ctaaaaatgg tattaagaaa tgtacacctg tttcagctac tttttgaaac
     1141 ttaccaagtc tcattattat acctacttta gattgtttta taactcttat acaagttaaa
     1201 cttattgcta cgactactac tattaataca atacttaaaa caattttagt acccatacta
     1261 taaatcctcc tttattctat tggtttgtca cattaactgt ggcgtctact tttttaacaa
     1321 ctaattttac cccttcaatt tttaatactt ctactgtaga acctttttct atcttttcac
     1381 catctataga aatagccgtc catgtttcat gttcaactac aactagacct gtgttatttg
     1441 gagaaatctc ttctgtaaca ataccttttt tagacatcat tgcttgtaaa ttagtgttag
     1501 atttatagcc tttatctttt tttactattt ttttagtagc tacgactaac attgctatag
     1561 aaattactgc aaatattaaa atttgtaaga aaatactctc tataaagcta cttaaaaata
     1621 taagtattac agctcctaca ctaaaccata tcaatgttaa gcttggtgtt aacatttctg
     1681 ctattccaaa taagactgct actatcaacc atattaactt catactatct accctctttt
     1741 catatgaatt ataaaaccct atataaatta tatgtttata caatgttttt gaatataaat
     1801 ttaaattaca aaacaataaa ataaattaat taattaatag tattatatca acatactttg
     1861 ttaaaattgt tacaaagtag tatcaaatat caccaattat atatccaaca actctttcat
     1921 tgttaatgaa atgttactta ttaaatccgt agcatttatc gtatacattg ctttgctcaa
     1981 tttatctcct atatagccat gtatatatgc tccactaact gtagcttcca atatatccat
     2041 accttgacct ataaatgaag taatcattcc aagcaaacaa tcacccattc caccagttgc
     2101 catacagcta tttcctgtag gatttatata tgttttttta ccatctgtta tgacagtttg
     2161 ataacctttt aaaagcacca ccagattata tttttgagca aattcttttg caacatctac
     2221 tctatgttct cttatatacc caattgaatc tcctgtcaac ctggccattt ctcccaaatg
     2281 aggtgtaatt acaaatctat ttttccattc taaaaactta taacatctgt ccttcataac
     2341 gttcaatcca tctgcatcta atactattgg gcaattagaa ttttctacta tcttaagtaa
     2401 tttatcaaat gtttgactat tatctcccat acctggacca aaaccaattg catcactaga
     2461 attaagtaat ttatttaatc tttcttcttc aaaatttaaa gtcattgctt ctgtaagtct
     2521 aatagatacc ttttcttgca catcttcact actaattaat gttacaagtc cactcccact
     2581 cttaacagca gattcagttg ctatgaaact tgcaccataa aatccttttg aacctgcaac
     2641 tatagatact tttccaaaat cacttttaaa accatgctta tctcttcttc taatattatt
     2701 ttttacaaaa gtcttatcag ttatgaactc ttcatcatgg tattttcttg taataaaatc
     2761 tggaacacct atcttttcta caactacatc acctataagt gcgctagtgt catagtttag
     2821 aaaacctctt ttataaaact caaagctaat cgttttgttt gctctaatac aagttcctaa
     2881 tacatttcca ttatcgctat ctatacctga aggaatatct atagaatacg tatttttgct
     2941 attatcgttt actatgtcaa taacttcttt aaaaatacca gttatatcac gttttaatcc
     3001 agtcccaaat atagcatcta ctacaacatc acttttagat actctatctt taaaaattgt
     3061 taaattttta ttttttataa tattttgttt ctcaacttta ataattttat ctacttcatt
     3121 ttcatctcca taattttttt cacttaaatc atttttatta atatttataa tattaattcc
     3181 catatttaaa agtacattgt aatttatttt agagcattca cttagttttt caagattacc
     3241 tactaaaaat acgtttactt cttttccata tgtatttaga tgtcttgcta ttccaagacc
     3301 atctcctcca ttatttccaa caccacatac aactgtataa ctattataca tgtctatatc
     3361 catatttttc attgcagata taaccgcatt ttccatcaat acaatcattg gaatccctac
     3421 ttcttctaca catgcagtat caacatttct gcttaattta gatgtaccta ttagcataaa
     3481 ttatcactcc tataaagtta aagacttctt aaatacttac caaaaatatc tattgaaatc
     3541 tttttaatcc cataaatatc ataaataaaa tcactaaaac taagagtttt gaaataattt
     3601 aaaataacaa aaaattttga attaaaacaa ttatttaatt tattatacca tataatttaa
     3661 gttattatgt tgatttaaaa cctatttaca ctaattatct aaatatctct ttatttttat
     3721 aatcttttat gacttattta tatttttcat ttttataaaa aaacaataat taagaatata
     3781 actatattct acatgtaaaa atttttttat ttaaactcaa ttttctgata atttgataat
     3841 ttttcaaaat ttatagttga cttttatttt ttactgtatt aaactaatta ctatacaaaa
     3901 cagaaaacaa aattttcaaa attattaaat taaaaggaga ggtaaatatg aagactttat
     3961 taaacaaaca agtacgattt tttagtagcc aatttagtta gggtttgtac ctaagttaag
     4021 acaaattgtc ataacataga tacaagcccc ttaggattat gatttttaag gattgtatct
     4081 atgttggcta tagtaattgt tgttaaagtt tttattttat catcataata tagagccaca
     4141 tggattatac tgtgtggctt attcgaattt ttgccataca gttatatata ctgtgtggtt
     4201 tttttatatc aaaatcattt tttaaaatca aatttaaaaa accatttaaa atattttcaa
     4261 aggggtgttt tatttatgtc aggtattata tctgtaatga cgcaaagttt aattttatcg
     4321 attatggcac tgggggttta tataacttac aaaatcttag attttccaga tatgtctgct
     4381 gatggtagct a
//
LOCUS       DAEOSP010000250         4363 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.254, whole genome shotgun sequence.
ACCESSION   DAEOSP010000250 DAEOSP010000000
VERSION     DAEOSP010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.254"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            337..2436
                     /gene="nrdE"
                     /locus_tag="KRH54_003319"
     CDS             337..2436
                     /gene="nrdE"
                     /locus_tag="KRH54_003319"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBG8580948.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLKKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGNKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2449..3420
                     /gene="nrdF"
                     /locus_tag="KRH54_003320"
     CDS             2449..3420
                     /gene="nrdF"
                     /locus_tag="KRH54_003320"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBG8580949.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3496..4122
                     /locus_tag="KRH54_003321"
     CDS             3496..4122
                     /locus_tag="KRH54_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBG8580950.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQKLHIGSYDNEPES
                     FDIMNEFCKENKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         1754 a          459 c          794 g         1356 t
ORIGIN      
        1 atttttaagc tatttagaga taatttaaat ttttaaacag gacataataa gcttgtattg
       61 ttttatattt gagttgataa aaatttcttt aaataaaaat aatgtcaatt ataaagtaat
      121 aaaagtttag atataaaaaa atttgtgtat atactatatg tagtatataa tagataaagc
      181 actgactata tgtagtgctt ttgtttttga aaaatactta ttgcatttct ataattatag
      241 attaattttg ggatttcaac tgataagtac caaaagaaat aataagcaaa ttaagtgaaa
      301 ttatggcaca agattatgtt aaaaggacgg taaataatga attggataga gcttaacaat
      361 caagtgataa ttaaaaatga acaaggcaag taccaattgg aaaaggataa ggaagcctta
      421 agtagttaca tagaagaatt tatacagcca aagttgaaaa aatttaataa cctagaagaa
      481 agattgaagt atttaataga tgaaggttat tattcaaaag aagtaataaa tcagtatagc
      541 atagatgtta tcaaaaatat atatgatatg attgataaac ataagtttga atttcaatca
      601 tatatgagtg caaataagtt ttatcaaaat tatgcattaa agagcaatga tggaaaggaa
      661 atattagaaa catataatga taaagtgtta attgtagctt tgacactagg aaatggcaat
      721 aaagatttag ctttaaactt ggcagataaa atagtaaaac aagaatttca accagctaca
      781 cctacttttt taaatgctgg aagaaaaagg gctggagaaa tggtatcatg ttttttacta
      841 tctgtagaag atagtacaga aggtatatcg tatgctatat cttcatcaaa tcacttatca
      901 aagattggtg gaggagttgc actaaattta tctaaactta gagcatctgg cgaatctatc
      961 aaagatatag aaggtgcagc tggaggtgtt gttggagtag ctaaaatgtt agaacaatca
     1021 tttagttact ttaatcaaat gggagctaga caaggttctg gtgcggtgta ccttactgta
     1081 ttccatcctg attttgaatt gttgatggat actaaaaaga taaatgcaga tgaaaaaata
     1141 aggcttgcta cattatcatt aggagctata ataccagata aatttatgga gttggcagaa
     1201 aaaaatgaag tagcatatgc attttatcct catactgtgt ataagaaata tggagtttct
     1261 cttgatgaga tagaaatgga tgagtggtat gataaattgg ttaatgatag tgatataaga
     1321 aaaaaagaaa taaatcctag gcagatgttg acaaaaatag cacagatgca acaagaaagt
     1381 ggatatccat atgtagtgta catagataca gctaatagag aacatacatt aaaagatgtt
     1441 ggaattatta aaatgtctaa cttatgttgt gagatatttc aatatcaaac accttcagaa
     1501 atagaggggt atggaggaaa aaatgaatgg ggacaagata taagttgtaa cctaggttct
     1561 ttaaacatag ccaatgtaat ggataataaa actatagaaa gtacagttga aacagcaata
     1621 agagcactgt catttgtagc agatagcaca gatataaaac cagtaccaac ggtaagcaat
     1681 agtaacagca agtctcattc tataggatta ggagctatga atttacatgg atacttagta
     1741 agagaaaata ttttatatac ttcagatgat gctatagatt tttctaatgt attttttgca
     1801 atggttagat actattcaat taaagcttct atgaaaatag ctatagaaag aaatcaaact
     1861 tttgaaggat ttgataaatc agaatatgtt aagggaagaa atagcaaggt tttatcaaaa
     1921 tactatgaac aatcttatct acctaagtca gaaaaggtaa gagcattatt tgaagggatt
     1981 tatataccaa caaaagaaga ctggacaaaa ttattggatg aggtaaaaga aaaagggata
     2041 tataatgcat atttaatggc agtagcacca actcaaagca ttagttatgt tcaaaatgct
     2101 acatcaagta taatgccaat tactgagcca gtagaagtaa gaacatatgg agactctact
     2161 acaatatatc caatgccatt tttaacaaat gataatatgt tgtattatca atcagcatac
     2221 aggatggata tgagaaaggt catagattta gtggcaacag ttcaaaacca tgttgaccaa
     2281 ggtatatcaa caacactgtt tgttacagat gaaaagacaa ctagagatat agctagacat
     2341 tacatatatg catataaaaa aggtttgaaa agtttatatt acacaagaac aaagatgaca
     2401 agagatactc atgagtgtct agtgtgttca gtatagggag agaaatatat gacatttaat
     2461 ttacataaaa tacataatgc agttaactgg aatagagaag aagatggatt tacacaagct
     2521 ttttgggaac aaaatgtaaa acaattttgg cttcctgaag aaatatctgt ttctaaagac
     2581 ataaaggtat ggaatgaact aagtaataaa gaaaaggaat tatataaaaa agtattgggt
     2641 ggtttaacat tactggatac aaaacagggg aataatggaa taccatctat gatgagttta
     2701 acagaaaacc tacaaagaaa agctgtcttg tcttttatgg ggactatgga agaaatacat
     2761 gcaaaaagtt attcttctat atttatgact ctactttcta atttagaaat agatgaatta
     2821 tttgaatgga tagaaacaga accaacatta caaagaaagg ctgatttagt tttagctcaa
     2881 tacgaaaata ctacaaatca agagggatta tacctatcaa tggttacaag tgtattttta
     2941 gagagtttct tgttttattc aggattcttt tatccattgt atctatctgg tcaaggaaag
     3001 atggtagcta gtggagaaat aatatcgctt attcttagag atgagtcttt acatggtaag
     3061 tatataggat tgctagcaca agaaatatac gattcatttg ataaaatgga taaggagatg
     3121 ttagaagaga agatgtattc tatactttat agccttatgg agaatgagat agagtataca
     3181 aatgttatat acagagaaag tgggcttgaa aaagaggttg taaacttttt aaaatacaat
     3241 gcgaatagag ctttagaaaa tctagggttt gagaagctat atactgtaga tgctataaat
     3301 ccaattgtat taaatggtct tagcactgaa accaaaacac atgatttctt ttcaactaag
     3361 ggtaatggat accaaaaagg agtgtatgag gaattagagg atgaagattt tataatataa
     3421 tttataatta tgacacactg ttgtcacttt tagcgtgata atatcaatgt ataaaatata
     3481 tatggaggta tttttatgaa atatgaatgg agaaaaagag ataaagagtt atatttacct
     3541 aaaaagaatc ctgtaatagt tgaaatacca gaacagaaat ttatcatgtt aaaagggagt
     3601 ggagacccaa attctaaaga atttactgaa gcaataggag tactttattc tttggcatat
     3661 gcaataaaaa ccatgcctaa aaaaggaatt gtaccagaag ggtattttga ctatacggtt
     3721 tttcctttag aaggtatatg ggataaggga gaaaaaagta aaggcttaga tgtacttatt
     3781 aaagaagatt taatttacac tataatgata cgtcagccag attttgttac acaagacctg
     3841 ttggataaag ctactgatat agtaaagaag aaaaaacctc ataatcttct tgaaaaagtt
     3901 agatttgggt ctattaatga agggctatgt gttcaaaagc ttcatatagg ttcatatgat
     3961 aatgaaccag aaagctttga cattatgaac gaattttgca aagaaaataa acttataaga
     4021 aaatcttaca cacatcgtga aatttatata agtgatgcta gaaaagtatc gcctgaatca
     4081 ttgaagacgg ttttacgttt tgaagtagaa agggaagtat aaatattttg aataatttat
     4141 aagagttata aaaagtgaag tctgttgaga cttcattttt tgatgcaatt ttaaaattaa
     4201 atttgatgtt tcataatatt ttaaaaactt tctactttta ttagtgtgaa taactgttgc
     4261 tattaaataa taatactaat tatataagta ttattatata atatttatag attattatat
     4321 aagtagatta ttagtaagat gtatgcatta ttaattaaaa taa
//
LOCUS       DAEOSP010000251         4311 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.255, whole genome shotgun sequence.
ACCESSION   DAEOSP010000251 DAEOSP010000000
VERSION     DAEOSP010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.255"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            78..959
                     /locus_tag="KRH54_003322"
     CDS             78..959
                     /locus_tag="KRH54_003322"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG8580951.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            1384..2385
                     /locus_tag="KRH54_003323"
     CDS             1384..2385
                     /locus_tag="KRH54_003323"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBG8580952.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTGRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            2502..3389
                     /locus_tag="KRH54_003324"
     CDS             2502..3389
                     /locus_tag="KRH54_003324"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBG8580953.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            3456..4205
                     /locus_tag="KRH54_003325"
     CDS             3456..4205
                     /locus_tag="KRH54_003325"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG8580954.1"
                     /translation="MLKVIATGYDGKMGKILADTIRENNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
BASE COUNT         1589 a          534 c          788 g         1400 t
ORIGIN      
        1 taaaattaat taatttgtac tataattatt attgtactta aaaaattaaa tttagataaa
       61 aaaggagatt tgattatatg ttatttaaag gttctgctgt tgctttagtt accccattta
      121 cagaggataa taatgtcaac tttgaaaaat taggtgaatt aatcgaatat cacattgaga
      181 atggcacaga tgcactagtt gtttgtggaa caacaggtga ggcgactact atgagtgaat
      241 ctgaaatttt tgctgtaata aaatatacag ttgaaaaagt taataagaga atacctgtta
      301 tagcaggtac tggttcaaac aatactatgt tgtctgttca tatgagtcaa gaagctgaaa
      361 aattaggggt ggatggtctt ttaataataa ctccttacta taataagact aatgagaaag
      421 gtcttaaact tcactttgaa acaatagcaa atagcgttaa gttgccaatt attttataca
      481 acgtaccagg aagaactaaa gtgaatataa agccatctgt tgtggctgaa cttgctaaaa
      541 tagataatat agttgctgtg aaggaagcaa gtggagactt agctcaagtt gcagaaatag
      601 ctaaattagt acctaaagac tttgctatat actccggtaa tgatgatact atactaccac
      661 tattatcatt aggtggaagt ggtgttatct ctgtacttgc taacatatgt cctaaagaaa
      721 cacatgattt agtaactaaa ttctttgaag gtgatattga aggctcaaaa aaattacaac
      781 ttgatatgga tgctttaatt gctgctttat ttatagaagt aaatccagtt cctgttaaaa
      841 ctgccatgaa cattttaggt tttaatgttg gtgatttaag acttcctcta gctgaaatgg
      901 aagaaactaa tcttaatgtg ttaaaacaag aattgacaaa ttttggattt aaattttagc
      961 aatatttata taaataagtt gattttttct taaatatgta ttattattag aataatcaaa
     1021 ttttaatatc ctattaacct agggtagagg tagctgtaac taagagtatc caatagtagc
     1081 tggcaggtgt taatgattgg aaaaaggatt tacagccgaa gaaaatattt cttgcaataa
     1141 tattttctgg gattgcacat aacatgtgta atactgtcac ttatttttgt aggtgatgag
     1201 ctacagggtt tactttgagt gataaattag acgtacaaag tatacctttg tacgtttttt
     1261 gtatataatt aaagttgtta atataatatt aaagtttttg gatatataaa aatattaaca
     1321 tatcttactt gtataactaa aataaaataa tgttttaata atttaaaatt ggggtgaatt
     1381 tatatgaaaa aagtaaatgt agcaatagtt ggtgctactg gaatggtagg tagaacattt
     1441 ttaaaagtat tagaagaaag aaattttcca atagaaaatc tatttttatt ctcttcttct
     1501 aaatcagctg gttctaaagt aatgttttgt ggtaaagaat atatagtaga agaattaaag
     1561 gaaacttcat ttactggtag aggcatacaa atagcattat tctctgctgg tggaagtatt
     1621 agtgaaaagt atgctccaat agctgcttca aatggaatct tagtagtaga taactctagt
     1681 caatggagaa tgaatccaga tgtgccttta gtagttcctg aggttaatcc agaagccata
     1741 aaaaatcata agggtataat agctaatcct aactgctcta ctatacaagc tatggttccc
     1801 cttaaagctc ttgatgataa atacaaaatt aagagagttg tttactctac ttaccaagct
     1861 gtatctggtt ctggtgttaa aggtgttgaa gatttagaga aaggaatgaa cggtaatcct
     1921 actggattct accctcatca aatctcttac aactgtcttc ctcatataga ttcattcact
     1981 gaaaatggat acactaaaga agaaatgaaa atggtaaatg aaactatgaa aatacttgat
     2041 aactacgatt taaaaataac tgcaactact gtaagagttc cagtaaaaaa tggtcatagt
     2101 gaatctataa atgttgagtt taacaatcca tttgagttag atgatttagt aaaaacactt
     2161 gaagaagctc caggtgttgt agtagttgat aatcctgcta aaaacgaata tccaactgct
     2221 gtagaatttg atgatagaga tgaagtattt gttggaagaa taagaagaga ttttagtgtt
     2281 gataatggtg taaatctttg ggttgttgct gataatataa gaaaaggtgc tgcaactaac
     2341 gcaattcaaa tagctgaaat ggctttatca tacgatttag tataatatac aaacttgtat
     2401 aaatatataa ttttgtaaaa tattaatagt aactcaaaaa caagcattag aagtaactgt
     2461 aatataaaac acaaaaataa aaaagagggg attgatttta tatgatattt aaaggttctg
     2521 ctgttgctct agttacacca tttacaaagg ataacaaagt tgattttgac aaattaggtg
     2581 aattagttga ataccaaata gctaatggta ctgatgctat agtatcttgt ggtactactg
     2641 gtgaagctaa tactatgact gatgaagaac aacttgctac tattaagtat gttgttgaaa
     2701 aagttaataa aagagttcct gttatagctg gaagtggttc taatgatact atgcactcag
     2761 ttaacttaag tcaagaagct gaaaaattag gagtagatgc tctattaata ataacacctt
     2821 attacaataa agctaataaa gctggactta agcgtcattt tgaaacaata gctaacagtg
     2881 ttaaacttcc aattatactt tacaatgtcc ctggaagaac ttgtgtaaat ataagtccta
     2941 gccttatagt tgaacttgct aaaattgata acatagttgc tgtaaaagaa gctagtggag
     3001 acctaggaca ggttgcagaa atagctagtt tagttccaga tgactttgct atctactctg
     3061 gtaatgatga tactatactt cctttattat ctttaggagg tcaaggagtt atatcagtac
     3121 ttgctaatgt ttgccctcaa gaaactcacg atttggtagc taaattcttt gaaggcgata
     3181 ttgaaggttc tagaaaatta caacttggta tggatgctct aatcgcagct ttatttatag
     3241 aagtaaatcc tataccagtt aagactgcaa tgaatttatt gggattcaat gttggagatt
     3301 taagattacc tctagcagaa atggacccag caaatttaga agtattaaag aaagaattag
     3361 ttaactttgg actaaaagtt aatgcatagt aaagattaat gcatggtaaa aagtaagttt
     3421 ggtcaaaagt tagcccttaa tcataggagg aaattatgtt aaaagttata gcaactggtt
     3481 atgatggaaa aatgggtaag attttagccg atactataag agaaaataat gaattagaat
     3541 tggtttgtgt tgcagctaga ggtctggata gctatgaagg cgaccttaaa atttatgaag
     3601 atatgagtac tatagttgag gaagctgatg tagttataga tttttctcat cactctaatt
     3661 tagataatat cttatcttac gttttaaaga ctaaaacacc tttagtaatt gctactactg
     3721 gatataatga tgatgaaatg aacaaaatac gtgaagcggc taaagttatt cctgtattcc
     3781 aatcatataa tatgtctctt ggtgtaaatg tattagtaaa attggttaaa gatgcagcta
     3841 aattactaga aggatttgat atagaaataa tagaaaaaca tcacaacaga aaagtagatg
     3901 ccccaagtgg cacagctgtt atgattgcta acgctataaa agaggttttg ccaaacgttg
     3961 aaaataatta tggtagatat ggtcgtagtg caaaaagaca accaaatgaa gttggtattc
     4021 atgctataag aggtggaaat attgttggcg aacatgatgc tctatttgct ggagacaatg
     4081 aggttcttac aataagccat caagctcaat ctcgtggtat ctttgcaagt ggttctatag
     4141 ctgctgccaa atacttagtt aagcaaaaac caggatttta taatatggac agcatgttgg
     4201 gataatagta taaatataat aatacacaat ataaattaaa catatgcaat atagatataa
     4261 taatacgcaa tacaaatcaa acatatgcaa tatagatata ataatacgca a
//
LOCUS       DAEOSP010000252         4308 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.256, whole genome shotgun sequence.
ACCESSION   DAEOSP010000252 DAEOSP010000000
VERSION     DAEOSP010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.256"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(746..1156)
                     /locus_tag="KRH54_003326"
     CDS             complement(746..1156)
                     /locus_tag="KRH54_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580955.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKFESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(1372..2754)
                     /locus_tag="KRH54_003327"
     CDS             complement(1372..2754)
                     /locus_tag="KRH54_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG8580956.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(2765..3124)
                     /locus_tag="KRH54_003328"
     CDS             complement(2765..3124)
                     /locus_tag="KRH54_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8580957.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(3329..4108)
                     /locus_tag="KRH54_003329"
     CDS             complement(3329..4108)
                     /locus_tag="KRH54_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBG8580958.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         1515 a          716 c          396 g         1681 t
ORIGIN      
        1 cctaaccctt taactatata tttttttctg gtattcttat ctactattat ccgtatattt
       61 attttatgat tactctttta acctaccccc tatactttat agtaatatgt ttttaacctt
      121 tttcattatt cccaatatgc tttataacta caaatactct ccccccttaa aaataataat
      181 aaaattaatc aataaattta taatcatatt attactctat gtattaagca attactatgc
      241 caataaactt tgattttttt aaacaaatct taaaaatcct aaaatgctaa ttttctttaa
      301 cttttaatga atctaataga taacatttaa ttttcgttat acttttcaga atatttctat
      361 tattattttt aagaaaaaaa aataatgtta acatttctta acatacatta aaaaatgtta
      421 acattataaa ttgggtttaa tacttttact ttgactcgat ttacttttat tctacttctt
      481 actttatttt taacttagac tttatttttt tattacttat ttgagtctta tttattttat
      541 tctcactcca tatgttcaat tacatttgtc taaattcttt gatactttta ttacataatt
      601 ttaacaagct taagtgttat ttgggtagtg cagttgataa ttatttaaat aatcatcaac
      661 tgcatgttta tatcattaca gtagcttaat gtatttaatc atacttactt aggattttga
      721 aatatcttag agattatatt taacattagt caattgctgc ataattgtaa gcaattgaaa
      781 tttgagaccc atcttcattt tctatagtga taggcttcat aatttttaat ccttcagttt
      841 ttatcttttc taattcttgt tccattttct taattgtttc ctcaaggtct ttttgactta
      901 catctttgac tgcattaggg tctttagcat agtcttttaa ttcttgaata tgtttagcat
      961 actcttcata agtataatgc tcaacaccat acttttccaa ttcagtaggg tcagttattc
     1021 ctgattttat tagagattca aatttaggta aagcaatatt agatgaatca tttccatttg
     1081 caaatactgc tccagttcct cctagtacca ttgcagatac catcattgca cttataaggg
     1141 atttttttat tttcataacc aatcttcctt tcttagagat ttatttgggt agtgtagttt
     1201 aaaatgacta ttgcaatagt tatcaaccac attcttagac tatcacagta gtcatgttta
     1261 tgtcaagcct ttttaaaaaa cttttcaata aaaaatacta tctcaaaaca aaattaatta
     1321 ttttgagata gtattttaat tctaaatctt ttatattact aagatttatg gttactctat
     1381 ccatgttcta tattgttctt tattaactgc taacaaattc ccttttcttg atatgacaaa
     1441 gcgattatca ttatagccac ttcgctcaat tgaaacttct cctgaatcag tataaagtac
     1501 atccttacta ttactagatt tatttacata cgtttcttca ctattctcag aattagttac
     1561 tttgctcata gatgattctt catgctctct aaattcaatt acagatactt ttgccgtact
     1621 aaaatgagtc tctccatcac ttttagcttt actaacaaga atatcagatt ctactttggg
     1681 agaatataaa tcagttgatt tactacttat tgaaaatcca attttatcac tttgttcatt
     1741 ttttgttaaa ccaaaagatt caacctcatt aatctcttta ttttctgttg caaaaacaag
     1801 acctgtccca caaactaata cagttgaaac taaaaaagta acataagttg attttttaaa
     1861 tttcataata ttaactattc tcttttctat ttttgtcttt ccaaatccac tacatattgt
     1921 cattgaaaat ggattattct cctctgctag acttattagt gtggttgcat aatcagccct
     1981 atattcttca ccaagtatac tcataacctt ttcatcacaa ctaaattcta tgtctttatt
     2041 tagtaaaaaa aacatcaccc agactaaggg attaaaccaa tggacaacaa gagctacaag
     2101 cataaataac tttaacagat tatcaaagct attaatatga accatttcat gagttaaaac
     2161 aaagtctaaa gtttgattat tagcataatt catttgcttt ggcaaaataa ttcgtggttt
     2221 taaaatacca gatgtaagtg gtgtcctagt aaattcagat atgcaaacaa taaatcttct
     2281 aaaacggcta ttcttacaca tccatttttt aatgaactca tgatttttta tatgaattga
     2341 agttttaaaa agattaatta ttttaaaata tctaaagcat aaaagtatgc taaacataat
     2401 agtaactata aaagacaaaa tgcccaacat ttccaacatc ataatatttt caatattaga
     2461 cattatttca aattcgtcag aattgttttt acctgtagag tcactagctt tcttaagagt
     2521 ataattttct gttgatgtat tacccaattc cacatcaaaa gtcgattcaa ttatactata
     2581 aatactaaaa ccagaattta aacttactgg tattagaagt ttaaataaaa caatcaacca
     2641 caataataca aatacctttt taggtaattt tttaaataaa catgctctta taaaaataac
     2701 tattaaaagt aatactccag aagtaaaact catttctaac aatttaatga acataaatct
     2761 tacctcactt taatttttca acaatttgtt taagttttgt aatctcatca cttgaaagat
     2821 tttctttatt aataaaagct gaaagaaatt tttctgaaga ccctccaaac attttattaa
     2881 ttaaagtttc tgtttcctgt ttttgtactt cctctttgct tattaaagct ttacaaacaa
     2941 aattcggctc aattctctct attgcacctt ttgacactaa tttttttata acggtatacg
     3001 tagtgtttct attccatcct gtttcttctt ttaaaatttt tactaactct ccagccttgc
     3061 tttctcctct ctcccataga acttccatta ctttaagttc tgaatcaaat aattttatct
     3121 ccatataaaa tcgctcctca aatatagtta tcacaatagt cattactata ataaaaaact
     3181 atcatagtag tctatactat gtcaatcatt tattttttaa taataaaaac tgtcttttta
     3241 aaaaattctt ataaaagaca gttttatcaa tgttatactt taatgtttaa ttaaagccat
     3301 attttattta aatatacaac tatctatctt agtacatagt ataagatttt tctttatcta
     3361 atagtatatc ctctacatct gggttttctt ttactaattg tctataaaaa ttacaattag
     3421 taacacccac atatggaatt aaccatagta gacctattcc aagagtagct attgaaagta
     3481 tataccatcc tataaaacta aagtttagta caaataattt aagtacattt cctttcatca
     3541 tatctaaact agcacccatt gatttaaata cacccatatt atccttgtct tcacaaatta
     3601 agaaagtagc ttgagaaaca tataatagca atatataaac tggtaacata agaacaagta
     3661 ttataagaac tagagttatt ccagtagaaa taatagtccc tattccaaca ttatcacttg
     3721 ataaatattc aattgctcca ctagtaagta aaactccaaa tgataaaaac gctcctataa
     3781 ttgtgattat aaaagaataa atataaacaa taattgattc aaataaataa aaaccaaaac
     3841 ttcttaaata aattctccca cttacaaaga tatctcctat cttgactttt tcatctttag
     3901 taattttcaa gaacatacat gtaaatcctg ctgaaacgca aaaagctatt acagccacta
     3961 caatagtagt caatggttca aaaggtaatc ctacaattga tattaatact atcaatgcaa
     4021 tatatactgc catagaaatt gcacatatcc cccagtttcc agataactga gctttagata
     4081 ctcgttttaa ttcttgcctt gataccataa aaaacacccc tttcatttta taaaaaaact
     4141 tttaaaaata taaataatct tttaacgtat aatattaata tatcatattt tcgactatat
     4201 aagatacaat tattcaccat gttttatcaa gatatgccat aattgtatta ctagttttat
     4261 atagtcaaca ttaccaaata catcaaataa atttgattaa ttattcaa
//
LOCUS       DAEOSP010000253         4305 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.257, whole genome shotgun sequence.
ACCESSION   DAEOSP010000253 DAEOSP010000000
VERSION     DAEOSP010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.257"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            231..1310
                     /locus_tag="KRH54_003330"
     CDS             231..1310
                     /locus_tag="KRH54_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG8580959.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINIQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKKSRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="KRH54_003331"
     CDS             1634..2002
                     /locus_tag="KRH54_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBG8580960.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHENAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2119..2922
                     /locus_tag="KRH54_003332"
     CDS             2119..2922
                     /locus_tag="KRH54_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8580961.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKFLRNIISK
                     MCGATSEGRRSMLKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3307..4197
                     /locus_tag="KRH54_003333"
     CDS             3307..4197
                     /locus_tag="KRH54_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG8580962.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFEAVKELNDSGIFCGILLTPMLPFLTDTKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDSCEKLLREECARYGILTD
                     MKEIIKAYKRSESYTQIKFF"
BASE COUNT         1643 a          471 c          810 g         1381 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt gtttatataa ataaatttat atagtttata gtttaacagt aggaaatatc
      121 gtatgagtga gaatatcgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagtatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg gtcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagcattta
      601 tcatcaagga tgaagagcgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggtt gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacctg tactgcctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgcaaagg attggtatgg taaaaataaa tccactaact
      901 ggattgtaaa gcatggttgt agaactctgc taaaaaaagg aaatcgtgat gtattagctc
      961 tttttggata tgatgatact acttcaataa atattcagga ttttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaaag agtaggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatccaggt gttaatccct
     1441 gccgattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tgcaataccc atataaatta gaatttgtag
     1621 aggagaaatg taaatggatg tatatagttt ttggaatgac actttggcac aagatgctgt
     1681 taagataaga aaatattttc atgaaaatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaagg gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaacca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggatga tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtgtttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccaa atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gataaacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggat taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaatttttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgctgaaa
     2641 atgttagaag gctacgaaaa aaaagaactg tgtcatctca tgtatctggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaataa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttaaatatat aaaaactaca tactcattag
     2941 gtaaattatg tagtatatgt gtttacagat ggttatagaa ataaatatgt aataaactac
     3001 ttaacactta aaaagtggat ttgtccagag cataatacaa agcatgatat ataaattttt
     3061 aagcgaaata catcaaaaga agtattagaa ttagtggaga taaaacagct attagagatt
     3121 ataaataaat agttttaaag gtactacgaa tatagaggaa ggtaaatcat acgttattca
     3181 aggattataa atgattagtt taaaagtact atcggatagg tctctgcata tttaaacctt
     3241 agtaaaggta gttaaagtag tagtttaaaa actaaaaaat tatataaagg aattaggtat
     3301 aagaaaatga ttgagagtat accagcaaaa caaattttac aaaaagtgaa atttgacaat
     3361 acaagatggt ttggaattga ctataatatg aatctctatc gtggttgttc tcatggatgt
     3421 atatattgtg atagccgtag cacaatatac aacatagaga attttgacaa agttagatat
     3481 aaagaaaatg tgatagaaat tttaagtaaa gaattgcgtt caaaaagaaa aaaaggtgta
     3541 gtaggcattg gagctatgtc tgacacatat aatccttttg aaaagcaact ttgtattaca
     3601 aagcaagcac tagatttgat ttcggaaaat cattttggag tgtctattga tactaaaagt
     3661 agtttggtgg ttagagatat accgattttg cagaaaatta aaaaaaataa ttctgctatt
     3721 gtaaagttga ctataaccac tgcaaatgat gaattatcaa agaaaataga accttatgta
     3781 aatccaagtt ctgtacgatt tgaggcagtg aaagaactta atgattctgg aattttttgt
     3841 ggtatattat taactcctat gcttccattt ttgactgata ctaaagatga aatacgagca
     3901 attgtagaaa aagcacacaa ggctaatgca aaatttattt attgcatgta tggtgtaaca
     3961 atgcgtagtg gtcagagaga gtttttttat gagcatttaa gagatatatc accaaaattg
     4021 gtacttaaat atcagaaaac atatggttta aactatgtat gtactgtaca aaataaagat
     4081 tcttgtgaga agttgttaag agaagaatgt gcacgttatg gtattttgac ggatatgaaa
     4141 gagattataa aagcgtacaa gaggtcggag tcatatactc aaataaaatt tttttaagat
     4201 aggctttgga tgagatataa tagtggtgat tgatgtgttg atattatagt tgattgttag
     4261 agaggatatt atagatgaat attagtattg atattctagg taatt
//
LOCUS       DAEOSP010000254         4284 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.258, whole genome shotgun sequence.
ACCESSION   DAEOSP010000254 DAEOSP010000000
VERSION     DAEOSP010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.258"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(25..1470)
                     /locus_tag="KRH54_003334"
     CDS             complement(25..1470)
                     /locus_tag="KRH54_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBG8580963.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDKNGYYPKRSGIDFYHTYKEDLKLLKALGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEEARYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYENVIRTNGEELREK"
     gene            complement(1492..1845)
                     /locus_tag="KRH54_003335"
     CDS             complement(1492..1845)
                     /locus_tag="KRH54_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG8580964.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(1874..3121)
                     /locus_tag="KRH54_003336"
     CDS             complement(1874..3121)
                     /locus_tag="KRH54_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBG8580965.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            complement(3129..3446)
                     /locus_tag="KRH54_003337"
     CDS             complement(3129..3446)
                     /locus_tag="KRH54_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8580966.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            3688..>4284
                     /locus_tag="KRH54_003338"
     CDS             3688..>4284
                     /locus_tag="KRH54_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBG8580967.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAI"
BASE COUNT         1444 a          778 c          461 g         1601 t
ORIGIN      
        1 gggttgttta tatttaaaaa ctatttactt ttctcttaat tcttctccat tagttcttat
       61 tacattttca taccaataaa aactatcttt tttataacgt tctttacttc catttccatt
      121 gttatctata tccacatata taaatccata tcttttttcc atttcttgag atgaacaact
      181 tactatatct attggtgacc acatacaata agctaatatt tcggttccat cagctatagc
      241 ttcacctatt tgttctatat gttttcctaa ataatctatt ctgtaactat catgaacttt
      301 tccatcttca aatttatcat gcattccaaa tccattttca gctatcatta ttggcttttg
      361 gtatctatca taatattgag ataaagtatt gtataatcct tttgggtcaa ttgcccaccc
      421 ccaaggattt gcttttaaat atggattctt tatattgtca gttggattca tggtgttttt
      481 acttgctgat actgttgtag aataatagta tgataaagct agaaaatcca tagtattttc
      541 ttttaagatt ttatcatcac cttcctccat aactatattt atgttatttt ctttgaaaaa
      601 tctcttaata tatactggat actctcccct aaaacttaca tcagtgaaaa aatattgtag
      661 cctatttctt tttaaagcaa atactacatt ttcagggtca caatcttctg gataagatgt
      721 acaatctgcc atcatcatcc ctatcatcat atctgaattt atttcttttc caagcttctt
      781 agtcaaagct gtagctatga attgatgatg aatagcttga tatctagcct cttctacatt
      841 atctacttgg tctacacaaa atgcaactga attaaatgat tcataatgta ttaaatttat
      901 ttgatttata actatccaat atttaacttt atttttatat ctatttagta ttaattctgc
      961 atatctaaca aagaaatcta ccacttttct attagaccat cctttatatt ttaatactaa
     1021 attcaaagga gtctcatagt gaagtagtgt tacaataggt tccataccat tatctattac
     1081 ttcatttata agattatcat aaaactttaa tccctcttca ttaggctcta aatcatctcc
     1141 atttggaaat attctagccc aatttattga aaatctaaaa cactttaacc ctagcgcttt
     1201 caaaagcttt aaatcttctt tgtatgtatg ataaaagtct attccactac gcttaggata
     1261 gtacccattt ttatctttca tagcaaactt tattctttct atagtcatat cagactcata
     1321 aggtttagat atatctctat ctttatcata catatgtaca tcagctaaac accaaccttt
     1381 gccaccttca ttccatgcac cttctaattg attagctgct acagctcctc cccataaaaa
     1441 acctttagga aacttaccca atttaatcac tccttatttt atttatttaa atcatttact
     1501 attatttacc ttatattcta tagactttac tattcctact aaagacatag ttatatttaa
     1561 ctcgctgaat atggtcataa gagtatcttg agcatgtgca aataacagcg aatgtgctct
     1621 tttctctcca ccagcttccc cttgtataat cttggtttga gctctatgtg cttctgtcat
     1681 aaaccctttt gctttagcta gttttaaatc agcatttcta taatcaaact tttccaaatc
     1741 attaagagca tgtttaattt ctgttctagc ctcacctgca cgaagtatta tttgcattgc
     1801 cacctctact aattcttcat ctattatatg ttcttcaatt gtcatattta taatttcctt
     1861 tcaatttcta aatttaaacc tcttggctct ctaattttat ctcttctttc tcataagctt
     1921 taaaaaatgg ataccatatc ataaatagca atactatatt tattgctaaa acaataagac
     1981 ctctaacatc ttgatttacc ataaaagtag atataccaaa aggaacatac cataaattaa
     2041 acaccttact tggaattgtc acaaatccca tactcattga taagtaagta gttattgata
     2101 ttactactgt tcctatccac ataggtaaca ttaaaaatgg gttccatgcc actggagctc
     2161 caaatattac tggttcattt atattcatta tagaagggac aatacatgct cttcctattg
     2221 cttttattct tccaactctg gataaacaca tcattataac taatggtaat gtagcaccca
     2281 accctcctaa agttatccaa cctgtttgaa taacttcgta aacatatata ttagttgcta
     2341 ctcctcctga tgctactgct gctgcattag ctgcaattgc accaagttga attgaataag
     2401 ttataggaga tagtacccac ccacttattc ctaaagaata tataaatact actaagaagt
     2461 ttaagagtac aaatccaggt aatgtctgtg ctatatctga tactggttta aatactctaa
     2521 ctattatttc aaacatgtca aagttgagtc cataaattaa tccccagcct acaaacatta
     2581 tcactgttat aggtaacata ttatcaaacc acttgcttac aaaatccgga agtgctgaat
     2641 cttctccgaa aaatgaaaaa gaaccaaata aagacattat taatccagag aataatcctg
     2701 caactagcga tacaagcatt ccttctcctc caaacctgtt gaagttaaat atagctcctt
     2761 cattcgtaat ctctggacga atcatcatta acattaatcc agcacctgca agaccagcta
     2821 ctacactcat actttcaaat ttcttattct tcattatttc cataggcaat aaaaatgcta
     2881 taaataatcc taaaagtcca aatgtatatt gatttattgg acttaaatca ggtaaaaagc
     2941 ttatgtaatt cttcaagaca gaaactatag ttactaatga gcctacaaat accattggca
     3001 aaactttcat tatagttgat tgaactgatt gtacccatat attcttagtc atattattta
     3061 caacaggagt aaatttttca cttaaaaaat ctataaattt attcataaac actctcccca
     3121 taaatatttt atttgtttga tattagttct agtgcttctt ccaaagcctt tgcaccatct
     3181 agtcttccat atgcagtttt agatatacat gctactggaa cgttatattc attagccttt
     3241 tgttgaattt cttccttcat ataatctaaa tgtggtccta gtaataaaca atctatttct
     3301 gataaatact tatctatctc tgactcactt ctcgctttta tatctgcttc tatacctttt
     3361 ttctttgcac tctttctcat actagttgcc ataaaacctg tacttgctcc tgaaccacac
     3421 gccaaaagca ctcttatatt tttcataata atacctccaa tatttttatc cattaattta
     3481 tttcttaatg aactttttat tttattatgt ttatattgta cttaccaaat aagttcattt
     3541 cacataaata tatttcctaa taataggaaa tcagttactc ttaattaaac atattaatat
     3601 agtataatgt atcttataat acgtacattc aattatataa ttatcatatc atccagattt
     3661 tattttaaaa ctatgcgagg gaatctaatg aaaacatcta aaaaagaact atttttaaat
     3721 ttcataaaaa cacaacatga ttggatagat tcatctacat tggctaatta tcttaatgtc
     3781 tcaacgcgtt caattagaaa atatgtaaac gaaattaatt ccaatggtga atttatatta
     3841 tcctctaaaa aaggatataa agtaaatctc aataataatt gtcaaaccaa ggttgatagt
     3901 tcagaaaata tatcacctga taacaggtta aatttaattt taaaagaact tatagttaat
     3961 agtaatggaa ttaatatatt tgatttaagt gaagaattgt ttgttagccc agctactatt
     4021 gaaggagata tagtcaaagc taataaattt ataggaagtt ataatttaaa gataaagcaa
     4081 tcgaaatttt tattgaaact aattggaaat gagtctgcaa agcgcaaatt aatgagctct
     4141 ataatattta aagaaacggg aagtgatttt ttaagcttat ttgatgtaca aaaaatatat
     4201 caagagtata atttgactaa actaaaagaa aatataatat atatattaaa aaaatacaat
     4261 ttgtttataa atgagtatgc tata
//
LOCUS       DAEOSP010000255         4283 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.259, whole genome shotgun sequence.
ACCESSION   DAEOSP010000255 DAEOSP010000000
VERSION     DAEOSP010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.259"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..914)
                     /locus_tag="KRH54_003339"
     CDS             complement(<1..914)
                     /locus_tag="KRH54_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HBG8580968.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNNGTIILHPQGDSLSKNIYKLLKQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFIAI"
     gene            complement(1190..3844)
                     /gene="adhE"
                     /locus_tag="KRH54_003340"
                     /gene_synonym="adhC"
     CDS             complement(1190..3844)
                     /gene="adhE"
                     /locus_tag="KRH54_003340"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBG8580969.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKYTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
BASE COUNT         1387 a          746 c          479 g         1671 t
ORIGIN      
        1 ataattgcaa taaatattaa tacaatagat aacactgcat atacagctct gttgattatt
       61 tgttttgaat tactaaatac aacagtttct ggaattgaaa aaattaaatt ccagtctttt
      121 atataattat tttcactatt cccattatcc atagtggcat atcctaaata tcttctttct
      181 tccaacatat ttaatacagt tgcacctgtt ttattttctt gcaattcttt ttttaatcta
      241 tttacttctt gaatatcatt atcctgtttt aacaatttat aaatattttt tgataggcta
      301 tccccttgtg gatgaaggat aattgtacca ttatttttta caacataaga tatactatta
      361 ttttcataaa aagatatatt aataagtttc atcagagtat tgatattgta agtacaatat
      421 aaaactccaa ctactttatc attatttaat aatggtactg aaatagcaat cttctttata
      481 gacttattta cttcatcaac atatggactt gaaacatact tcttaccttt aattgagttt
      541 ttgaaaaatt ctctatcttt cactgaaaac ttatcacctt tggtattttc agcattacca
      601 tttatatcaa ctattgccat agtgtaaaat ttttgatttt ccatttcatc taaaagattt
      661 ttcatttttt cctcattagg tatatctgga tttgaagcat aatagttaga aaaaatattt
      721 aaaacatcta aatctttttc aatttgtctt tgtgctagtt gagagccttg tactgcaact
      781 tcttttatat gtatttttgc ctctgacata agaagatgtt ttgtatcttc tatatagata
      841 taccccattg tcaaaaataa tgatgttaaa aaaagaattg taattaatgt aaaactttta
      901 tttctccctg tcattcgact cctcccctaa ttagtcaata cttcatatag ataatatatc
      961 ctttttatac caattcatca tttatattgt atataactat tcttaaatgt tacacataaa
     1021 atcaacataa taaccataaa aacattcttt tattattata ataacactat ttaattgaat
     1081 tttaaattaa agaataaaaa ctatccattt tctcaaacaa aaaaactctt atacaataaa
     1141 actttgtttt atgtataaga gtttttattt tatctatttt atagcaaact tatcttccat
     1201 aatatgaatt taaatacata tcttttattt cattcataag tggatatctt ggatttgctc
     1261 ctgtacattg gtcatcaaat gcttgaatta ccatttcatc tattgaatcc ataaacttag
     1321 attcttcaac gccaaactct tttatagatt taggaatacc aactttaagt tttaaatctt
     1381 caatagctac caataaactt tggaatttct cttcatcaga atttcctttt aatcctaaaa
     1441 atgaagctat ttcagcatat ctctttaatg cttctggata tttatattga gagaatgctc
     1501 ccattttctt tggtgcatct gaagaattaa atttcataac ttctgttatc aaaagagcat
     1561 ttgcaacacc atgtggtaca tggtggaaag cacctaattt atgagccata gagtgacaaa
     1621 cacccaaaaa tgcatttgca aaagccatac ctgccattgt agaagcattt gccatttttt
     1681 ctctagcttc tgggtctttt gcaccattat catatgcacg tggaagatat tcaaatatac
     1741 tcttaatagc ttgtaaagct aatccatttg tataatcggt tgcaagcata gatacatatg
     1801 cttctaatgc atgagttaaa gcatctatac cagaagcaga tgttaagctt tttggcatat
     1861 tcatcatcat atcagaatca actatagcca tatttggcat cagttcataa tctgctaatg
     1921 gatattttac tcctgtatct tggtcagtta taacagcaaa tggtgttact tctgaaccag
     1981 tacctgctga tgttggtata gctacaaaat tagctttttc acccatttta gggaatgtat
     2041 ataccctttt tcttatatcc ataaatctca tagctaggtc ttggaaatct acatctggat
     2101 gctcatacaa tgtccacatt atctttcctg catccattgc acttcctcca cctatagata
     2161 ttattacatc tggattaaat tctctcatag ctttagcgcc tatcttagca cattccaaag
     2221 ttgggtctgg ttctacttca aagaatgtag tgtacttaat attcatttca tctaataaat
     2281 cagtaactgc tttagtatat ccattattgt aaaggaatgt atctgttaca ataaaagctc
     2341 tcttcttatt cattacatct ttaagttctt ttaatgctac tcctaaacaa ccttttttga
     2401 aataaacttt ttctggtgct ctaaaccaaa gcatattttc tctcctctca gctactgttt
     2461 taatattaag taaatgctta actcctacat tttcagatac tgaatttcca ccccaagacc
     2521 cacatcctaa agtaagagaa ggagctagtt taaaattata taagtcccct ataccacctt
     2581 gagaagaagg agtattaatt aaaattctac aagttttcat ggctgaagta aacttatcaa
     2641 gtttttctct ttgatttaca tcatcaatat ataatgaaga tgtatgccca aatccaccat
     2701 cttctactaa tttttctgct tttctaactg catcatcaaa atcacttgcc ttgtacattg
     2761 caagtactgg tgaaagtttc tcatgagcaa aagcttcttc tatttcaact gattctactt
     2821 cacctattaa aaccttagta tctactggga cttcaaagcc tgcaagtttt gctatagtac
     2881 aagctgtttg cccaactatt ttagaattta atcctccatt tactaaaatt atatttctta
     2941 ttttttctgt ttcatcttta tctaataaat aagcaccacg ctctttaaat tcttttttga
     3001 cttcattgta tatattttct aatactataa ccgattgttc agaagcacat atcataccat
     3061 tgtcaaaagt cttagataca agaattgagt ttacagcagt ttttatatct gcactatcat
     3121 caattattgc tggagtatta cctgcaccaa ctccaatagc tggttttcca gatgaatatg
     3181 ctgatttaac cattccagga cctcctgtag caagggtcaa atccacttct ttcataacag
     3241 ttgtagttaa ttctaaagat ggctcatcta tccatcctat tataccttca ggagctccag
     3301 ctaaaacagc agcctcaaga acaacttttg cagcctctat agtagaattt tttgctcttg
     3361 gatgtggaga gaaaattatt ccatttctag tttttaaagc taaaagtgct ttaaagatag
     3421 cagtagaagt tggattagtt gtaggtacaa ctgctgcaat aacacctatt ggttcagcaa
     3481 ttttagtcat accaaatgct tcgtctttct ctataacacc acaagtttta gtttctttat
     3541 atgcattata tatatactct gaggcataat gattctttat aaccttatct tcagcaattc
     3601 ccataccagt ttctttttgt gccattatag ctagaggaac tctttgctta ttagctgcca
     3661 aagatgctgc taaaaaaatt ttatctacct gctcttgact atatgtagca aatatttttt
     3721 gagcttctct gatttttgct agttttttaa ctaagctttc aacgttattt acagtatcta
     3781 aaccaactga cataacttca ttttcttttt tattttctat tattacttgt tctttatgag
     3841 ccattttaac aacctcctga gatagttttt tatttattat gttaatttat taacattata
     3901 gcatttactt ttcctatttt caagttattt ttttaacatt ttatttttta tctaatataa
     3961 catcatttct actaataata tctagcttta caaatactta atttaactag atatcacttt
     4021 attttttacg caaatcacta atgaaatcat ctaaaaactt aaggataacg ttattttttt
     4081 aacgtttata tttttgtatt ttttgcaaat aaaactttat aaaaagaatt tttaatatga
     4141 tactctatca agtttaaaaa taaaaatcct tgaaaatgaa aattaaaact taattaacaa
     4201 gttaaagtaa atatagttaa atgtacaaac atttacaaca tatagtataa ttaaaaaaat
     4261 atatattcat gatttttata taa
//
LOCUS       DAEOSP010000256         4240 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.260, whole genome shotgun sequence.
ACCESSION   DAEOSP010000256 DAEOSP010000000
VERSION     DAEOSP010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.260"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            100..852
                     /locus_tag="KRH54_003341"
     CDS             100..852
                     /locus_tag="KRH54_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBG8580970.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            839..1720
                     /locus_tag="KRH54_003342"
     CDS             839..1720
                     /locus_tag="KRH54_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBG8580971.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDTSNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNNITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(1946..3301)
                     /locus_tag="KRH54_003343"
     CDS             complement(1946..3301)
                     /locus_tag="KRH54_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBG8580972.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         1657 a          616 c          522 g         1445 t
ORIGIN      
        1 tttttttctt aatatagttg acattttttc ccttaaatta taaaatcaaa ttaataacta
       61 cataggtagt tactaatctt ttatcaaagg agtttctata tggataatat tattaatgaa
      121 ttacaaaata taggatttac taaatacgaa tctcaaatat acatatcatt attaaaggaa
      181 agccctttaa caggatatga aataagtaaa ttatcaggtg taccacaatc aaaagtctat
      241 gaaaatataa caaagctatt aaacaataat ataattatca atgttggaac tgaccctatt
      301 aagtacatac caataaaccc taatgaatta ttaaaaaata agaaaaaaga atttgacaat
      361 tctatggata atctaaagaa tagcttacag atattaaata aaagtaaaag tgtagaatac
      421 gtattaaaca taaaaggaga taaaaatata ttaaaaaaag caatagaaat gataaaaaat
      481 gcaaaacaag aaattataat ttcttgccct tatgaagaaa ttcttgaatt aaaaaaagaa
      541 ctcctaattg cttacaaaaa aaatattaat attgaaatac tacttttaga aaacaaaagt
      601 ctgaaaggtc ttgataatat tcatattcat ggaggtaata aaactacact tgaaaataaa
      661 atgctttaca aaggaattat tttgattgta gataatgatg aaatattgac aggaaactta
      721 tcagaaggca gtgaggccat ttcaatttgg acaaaaaact ctaacatttt atatattgga
      781 gttcaataca taaaacatga aatttatatt tcagaacaaa taaaaggagg ttataaaaat
      841 gaacaaaatt aagattaaac aagttgatgc ttttacaact ataccttttg gtggaaatcc
      901 tgctggtgtc gttactgata cctctaacat ttctgatgaa attaagcaga atgtggctaa
      961 agaaatgaat ttatcagaaa ctgcatttgt ttcagagtca aatattgcag attttaaagt
     1021 acaatttttc acaccaggtt tagaagttga tttatgtggt catgcaacaa taggtacttt
     1081 ccatgcactt ttggaagaag ggaagcttaa tactaataaa agtatatttt atcaagaaac
     1141 aaaagctggt gtattatctg tagagcttaa gaaaataaat aatgaaaata tatttatgat
     1201 ggaacagaat acacctgaat ttgaaaattt agataattgc agaaaagaac ttgcacaaat
     1261 gataggtgtg catgaagata gacttcttga atatcctatt ataaaggtaa atactggact
     1321 ttggtggctt gtatttggtc taaaatcttt agatgattta aaaaatatct ctccagattt
     1381 aaataaaatc aaaaaattta gtggtgataa taatataatt ggtataactc cattttgtat
     1441 agaaactata gataaaaagt gtgactacca tttaagatct atagcaccat atgttggagt
     1501 tacagaagac cctgtatgtg gaactgggaa cgcttgcgta tcatcttata tagtacatca
     1561 caatatttta gataaaacta gttttattgg tgagcaaggt aactttataa acagacctgg
     1621 aaaggtatat gtaaatataa ctagagaaaa caataacata acttctgtaa aaattggagg
     1681 aagtgcttat actgtcttaa gtggtgaaat tatgtattaa tcccatacct aataaaaaca
     1741 aataaaaata ctagctaact aaagctagta tttttatttg ttatttttat ttaataatca
     1801 tactctaaca aatattataa ttatatatat cataatattg tatttagaag taaaatttta
     1861 tttttattat taattagtta taaatcaatc taatacaaca taattacagt agattaacct
     1921 ataaaacata ataattgctt actgattatt tatcgtcctc aacaagtcta cttaatgtct
     1981 tatcagataa gaataataat atcgctgcaa caaatagtat tactggaatt atagtatata
     2041 ctttaatcat atcaaattta agagtaaatg catatatata tccatatcct ttaccagcta
     2101 taaaagtagc aaatgtccat actcccataa gaacacctag aagcttctta ggtgcatact
     2161 tacttacaaa agagttacca agtggtgaga ataacatttc accaagactt aaaagaatac
     2221 caaacatgat tatccatagt atactagctt tagcagtttc aggagctcca actcctcttg
     2281 aaaattctgc accaacaagc attaagaatg caagacctaa gaatattaat ccaagacctg
     2341 tctttttaaa taaactcata tctccttgtg gtctactagc aagtttaaac cataatgctc
     2401 caagaactgg tcctaatact atacatacaa gtgaatttaa tgagtcaaac caagcaagag
     2461 gaacatcaaa tcctccaaca ttcatgttta caaatgctgc accataatca taaactgcaa
     2521 gataagttag ataccagaat acccagaaaa ttacagagaa gaatgatata agtataatag
     2581 cccaaattct cttcttatcc atgcttgtaa gaggagactt atctttttct tcaatttttt
     2641 cagcaactac accttcttta aaaggtctct ttcctgcatt cccaaggaat ctccatccat
     2701 atatgaacca tattgcacct attacacaaa gtacagctgc tataaagaaa cattgagaaa
     2761 atccaagtac accatttttt gcaaatgttt taaggtaaag aataccaact gctatagtac
     2821 caataaaaga acctatatta acaaaagaat attgtactga aaatgctgta tcaagttctt
     2881 cttgactatc aaaaagctgt ccatttaccg cagatacgtt acctttaaag aatgcagtac
     2941 caattgaaac taagattatc attgcattta ccatagaaac tgtagttgca atagaaccaa
     3001 gatagtatcc tacccccatg ataagcatac ctataggtat agtatatcta gctcctatcc
     3061 atctatctga tatatacccc ccaattatag gtgcaaggta tgtgaatgca acaaggttgg
     3121 cttgcattat agcagcttgt cctttatcta ttccaagacc accatgaact atagctgttg
     3181 taaggaatat atatattaag tactttgaag cataatatgc tgctctttca aatgagaaag
     3241 ttgttgagca gatataaaat cccataggaa atttaccctt cttttttact gttgagctca
     3301 taattttaaa tcctttctta attgtatttt tattttttac gttttgtcca aattctttct
     3361 tttatgaaag atataatatt attattttgt aaaacaattt gtaattatta ttaagataat
     3421 tgcgcattta tttgtagagt acatattctc atggaataaa attaatacac ttcttaatca
     3481 taaatttaaa ttatataaat tcactatttt cagatatagt tataaattaa tatattctca
     3541 tctaatacca tgaaaaaacc ttcgccatat gcaattctta ttttttacgt gatagtattt
     3601 tacaaactaa aatccccctt ttgtagcaac ttatgtaatt acattttagt gcttttttaa
     3661 aggagtctcg aataaagcaa catatatttt gttcaaataa tgtttttatt tattttttaa
     3721 atttatatat tacttcaccc aaccaattct caacttaaat aactaaacta ctatctattc
     3781 acagattagg cttatttctt acacattagt ttataataaa cactgtgaat ctaaacataa
     3841 aaactgttat tttaaagtaa ttacccatat attcgctttg atactattta ttgaggctat
     3901 actctttatg tataatatat tatttataaa atacctcaaa atatattatt cataaaatac
     3961 ctcaaactga aaactttttc acgaatattt atttttcacc tccccttttt tatttcacca
     4021 tgtaaatcgt accataaata tgcacttata tgcaagaaaa aaatctatta atcgacagta
     4081 aataattaga aaaattcaaa ttatatttat caattatatt atataaaata tttcctatga
     4141 aaatataaat taaattaatg ttcagaatag tttttagata tgaaatcttg atttttttat
     4201 tactttactt atttgctcaa atatattttt gtatgcagta
//
LOCUS       DAEOSP010000257         4112 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.261, whole genome shotgun sequence.
ACCESSION   DAEOSP010000257 DAEOSP010000000
VERSION     DAEOSP010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.261"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            397..1872
                     /locus_tag="KRH54_003344"
     CDS             397..1872
                     /locus_tag="KRH54_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG8580973.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1959..2372
                     /gene="speD"
                     /locus_tag="KRH54_003345"
     CDS             1959..2372
                     /gene="speD"
                     /locus_tag="KRH54_003345"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBG8580974.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2402..3253
                     /gene="speE"
                     /locus_tag="KRH54_003346"
     CDS             2402..3253
                     /gene="speE"
                     /locus_tag="KRH54_003346"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBG8580975.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSCYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3243..>4112
                     /gene="speB"
                     /locus_tag="KRH54_003347"
     CDS             3243..>4112
                     /gene="speB"
                     /locus_tag="KRH54_003347"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBG8580976.1"
                     /translation="MKNNFYHRNTFMSMNKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDAAVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDK"
BASE COUNT         1664 a          442 c          728 g         1278 t
ORIGIN      
        1 aaaattattt agtccaacta aaccaagaat tttaatatgt gaaataagaa taatataaaa
       61 gtttagtaat aaaatactta taaattaaaa atagtaaact caaaagctta ctaaaactca
      121 aaaaagtaaa aaaaccaaaa taataaaaaa taagaaagtt tataaatact aagttttaaa
      181 aaaattaata taaacaacaa aaaattaaaa agtttataaa tattgatata gctaatgtat
      241 atgtttttga gtaacttcaa aaaaacactt gtaaaaatgc tgagatgtta atataataaa
      301 tatcatgagt ttaataaaag taaaaataaa aaatagaaat aataaataat aaatattaaa
      361 aatatttgaa tatagagagg agatgaattt gaaatcatgg atagcgagtt tgcaaatgca
      421 gttgaattaa gctatgagtg tgctccatta ttagagtcgt taaaggaata ctcagagaaa
      481 gatatagctt gttttgatgt gcctggacat gtaaaaaata gaggtgtagc tatattaaat
      541 aaatatctag gtgaaagtct catgaaaatg gatataaatt catcaccaac tatggataat
      601 gtatctgcgc caaatgggat tataaaaaat gcacaagatt tgctagcaca agcatatatg
      661 gctgatgagg cattctttat aacgaatggc acaactcaag caatacatgc aatgattttg
      721 agtgttataa aaccaggtga aaaagtatta cttccaagaa atatacataa atcagtaata
      781 aatgcgttaa tactttgtgg aggaatacca atatttatac aaccagaatt tgatgaaaaa
      841 ctaggtataa gtttaaatat tactcttgaa aaagttagaa cagagataga aaaagattgt
      901 aatataaaag cattattttt ccttaatcca acttactatg gagtttgtgc agaccttgag
      961 agtattatag aactatgcca taaaaataat gttttagtgt tagttgatga agctcatggt
     1021 gcacattttc catttcattt agatttacct ccttctgcaa taagtttggg agctgatatg
     1081 gtagctgtaa gtatacataa aactggagga gcattgacac aatcttcagc actcttattg
     1141 aatagagata atgtaagttt tgaaaaggtt cttcaatcaa tcaatatgct acaatcaaca
     1201 tcagcatctt atttgcttat ggcaagtata gatggtgcta gagttaatct agttgagaat
     1261 ggtgaaaagc aactgtcaaa agcattaaat ctatctaggt atgcaaaatc taaattaaac
     1321 aaaattgatg gaataaaagt tttatctaca gaaatattga aacaaaaagg tgttaaattt
     1381 atagatgaaa caaaattgtg tataaatgta aaagaattaa atttgactgg atttgaagta
     1441 tacgatttat tatataaaaa tttttcaatt caagtagaat tgggagattc atataatata
     1501 ctggcacttg tgtccattgg aacaaataaa tcagatatag atagattagt aaaagcactt
     1561 tctataattg caaagattta tagaaaagaa tcaacattaa atgaatttaa tatggttcaa
     1621 ataaatccaa ttattaaact taatccaaga gaagcttttt atgcaccaaa ggaaagtgta
     1681 gaaatcaatt catgtataga tagaatttgt ggagaatcaa taatggcata tccaccaggt
     1741 ataccgatta ttgctccagg agaattgata acagaagaaa ttatggagta tattattttc
     1801 ttaaaaaata gcaatgcata tcttacagat gtacaagata aaaacttaga tagaatatta
     1861 gtaataaaat aaattacttt attttgcaag ggcaagtatt tgttaagtag agtttatcaa
     1921 atagaactta ttaaaagtat aaaggagttg gggttgaaat gaaatttgaa acactaggaa
     1981 gacatatact agtagaatac tataattgtg atgaaaacgt acttaaagac ccacgtctta
     2041 tagaaaattt tatgaatgat tcagcattaa atgcaaaagc gacaattgtg gattctgtat
     2101 tccaccattt taatccttgg ggggtttcag gagctgttat aattgctgaa tctcatttga
     2161 caatacatac atggcctgaa tatggttatg cagcagcgga tttttttact tgtggtgata
     2221 tagacccatg gaaaagtttt gaattattag agcaactttt aaagtctgaa agaagcgagt
     2281 ctacagaaat tccaagaggc ttaactacaa aaattaaaaa atattcaaaa aaagatttag
     2341 gtaagattac tcataaacca gaagcagtat aaaacataca agagaaagtg aggattatta
     2401 tatggaactt tggtatacag aagaatggac agaaaatgta agattttcta taaaagtaaa
     2461 taaacatcta tttgaaggaa agtctcaatt tcaaaggata gatgtatttg atagtgatga
     2521 atttggaaaa tttctaacaa tagatggatt aatgatggta acatataaag atgaatttat
     2581 ataccatgag atgataactc atgttcctat ggctacaaat ttaaacataa agaaagtatt
     2641 agtcataggt ggaggtgatg gaggtacagt aagagaatta agctgttatc ctcaaataga
     2701 aaagattgac atggttgaaa tagataaaat ggttgtagat gtatctaagg agtatatgga
     2761 tatatgctca tgtaaattag atgataaaag agtgagttta tattttgaag atggtgttaa
     2821 ttttgttaag tgtgcacatg ataaatcata tgacctgata atagtagatt caactgaccc
     2881 aataggacca ggcgaagggc ttttctcaac tgatttttat aaggattgtt atagaatact
     2941 gactgatgat ggaattttag taaatcaaag tgaaagtccg tattttgact ttaatgctaa
     3001 agagatgaaa agagctaata aaaagttaaa acagatattt ccaatatcag aagtttatca
     3061 agcacatata ccaacatatc catctggtca ctggttgttt ggatttgctt caaaaaaact
     3121 taatccagta aaaaatcaag atagaaatgg atgggagaaa ttaagtttaa aaactaaata
     3181 ttataatagt gatatacatt taggttcgtt tatgttacct caatacgtga aggagatgct
     3241 agatgaagaa taatttctat cataggaaca cttttatgag tatgaacaaa aattatgaag
     3301 aatctaatct tatagtattt ggtgttggat ttgatggaac tacttctaac agacctgggg
     3361 ctagatttgc aagtagctct atgagaaaag aattttacgg tcttgagaca tatagtcctt
     3421 ttttagattt ggatttagag gattataata tatgtgatta tggagattta gaaattagtg
     3481 ttggaagtac agaacaagtc ttaaaagaaa tctatcaaga gacatataag attgttagag
     3541 attcaaaggt accctttatg attggaggag agcatttagt tacattacca gcctttaaag
     3601 cagtacatga aaagtacaat gatatatatg taattcattt tgatgcccat actgacttga
     3661 gggaagaata taataatagt aaaaattctc atgcaacagt aattaaaaga atatgggata
     3721 ttgtaggtga taataaaata tttcaatttg gtataaggtc tgggacaaaa gaagaattta
     3781 aatttgctac agaagaaaaa cacacataca tggaaatagg aggaatagat acgtttgaaa
     3841 atatagttaa tatgctaaat ggaaagaata tttatctaac tatagattta gatgtattgg
     3901 atgcagctgt gtttccagga acaggtacac cagaacctgg tggtgtaaat tatagagagt
     3961 ttcaagagat ttttaagatt ataaaaaact ctaatataaa tatagttggt tgtgacattg
     4021 tagagttaag ccctgattac gatacaacag gtgtatcgac agttatagct tgtaaaatct
     4081 taagagagtt atgcttaata atatctgata aa
//
LOCUS       DAEOSP010000258         4112 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.262, whole genome shotgun sequence.
ACCESSION   DAEOSP010000258 DAEOSP010000000
VERSION     DAEOSP010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.262"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            90..455
                     /locus_tag="KRH54_003348"
     CDS             90..455
                     /locus_tag="KRH54_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8580977.1"
                     /translation="MKIRAEYTCLLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            533..1087
                     /locus_tag="KRH54_003349"
     CDS             533..1087
                     /locus_tag="KRH54_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBG8580978.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIITKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            1307..1981
                     /locus_tag="KRH54_003350"
     CDS             1307..1981
                     /locus_tag="KRH54_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBG8580979.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      2336..2432
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2486..2662
                     /locus_tag="KRH54_003351"
     CDS             2486..2662
                     /locus_tag="KRH54_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580980.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(2850..3089)
                     /locus_tag="KRH54_003352"
     CDS             complement(2850..3089)
                     /locus_tag="KRH54_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580981.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(3223..4032)
                     /locus_tag="KRH54_003353"
     CDS             complement(3223..4032)
                     /locus_tag="KRH54_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8580982.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKIYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
BASE COUNT         1503 a          570 c          490 g         1549 t
ORIGIN      
        1 acataaaatt tatatacagt tcatatatat ttgtcatatc acaagacaag tgatataatt
       61 tattatcatt ttactggggg tgatatgcta tgaaaataag agctgaatat acatgtcttc
      121 ttgaaattgt acatgacata atcaaaggaa aatggaaaac tatccttata tttgaattaa
      181 gacaatctaa taaaacattt tctgaattgc aacataatat ttatggaata aatcaaaaaa
      241 tgttgattca acaattaaaa gagttacgtg attttgggat tattgataaa ataagttctg
      301 ctggatatcc attacatgtt gaatattttc ttactaatcg tggtaaaaaa atgttaaaag
      361 cagtggaaat aatgcaagaa ataggaatcg aatatatgct tgaacatggt caacaagaaa
      421 ttctagataa taaaggaatt tgctacaata agtaatccta tacataccaa aaagtaagta
      481 atttacattt tttacaatag tattataata aaaataaata ggaggtataa tcatgtctat
      541 agctttaata ttgattgata ttcaaaatga ttattttaaa aatggaaaat gtgaattatt
      601 tcaatcagaa gaaactgctg aaaatgctaa aaaaatacta ctgtttttta gaaaaaacaa
      661 tttaccagtt attcatatac agcatatatc aacagataaa acagcttctt tttttcttcc
      721 taatactcat ggttcagaaa taaataaaat tgtttttcca ctagataatg aagaaattat
      781 aactaaacac acaccagata gtttctttga aacagatttg caaagtatcc ttgagaaaaa
      841 aaatattact aatctagtaa tctgtggaat gatgagccat atgtgtattg atacaagtgt
      901 aagaacagca aagaaacttc gatacgacat aactctaata agcgatgcat gtacaacgaa
      961 gaatttatct tggaataata tagaaattaa tgcaaatact gtacatcaag tattcatggc
     1021 atcactacag aacagttttg cagatgtaaa aaacacagat aaatttttat ctaactatca
     1081 caaataaaaa catacactat gttaaaagag caagtttgat ttatttaagc ttgctcttta
     1141 atcatttaaa attagatgct ttctatcatt tttatataca taatatacgt ttatacattt
     1201 tatattcata tataactatt atataaattt taaactttac tatattcaca taaaaaatgt
     1261 agtattatag tatatataat attatataga agggggtgct agataaatga accatcatat
     1321 attgattcaa tttgaatata tagcaagaat cattactgct ggtttctgtg gggctttaat
     1381 tggatatgag agaaagaata gactaaaaga agctggaata cgcactcatt ttatagtggc
     1441 attagcttca gcacttataa tcattgtttc caagtatgga tttaatgata tagtgggtac
     1501 tccaggtatt ggtctagacc cagcccgcgt tgcagcacaa gttgtcagtg gtataggttt
     1561 tttaggtgct ggattaatat ttattagaaa tcaatcagta agtggtctta ctacagctgc
     1621 tggtatatgg gcaacagctg gtacaggtct tgccattggc tctggattat acttagttgg
     1681 tattgtttca agtatcatta ttgttattgt acagataaca cttcatagag ataggaaatg
     1741 gatgaaactc cctacagcag agcaagtttc tcttaaaatt tctaacagca ctgattccat
     1801 agaatatatt gaaggtaaat ttaaaaaatc taatattgaa attataaaca tgaatattaa
     1861 acgccttgat ggggattggt tagaagtagt tatatatact aagttaccta aaggatataa
     1921 aaaaacaaat ttattaaaat tatttagcga taatcctctt atagaaactt tagaaatata
     1981 atataaaaat tgaagcttaa aaataataat atatttaatc aaggtcaata tataaataat
     2041 tacgggatga gtctatataa aattcatccc ttgttttttg ccattcatat atattttttc
     2101 aagatatttt tgttcatcta ttatctagcc aagtaactct tgtgaactaa actttttaat
     2161 acaaaaactt caatacaaaa ctaaatataa tttttattaa atattctaca tgtatagttt
     2221 atttcttgag tgaaaaatag atttcatatt gttaagatac tgttaaatat ttcaattttc
     2281 tgacaagtat gttgaacaat aacttatcct agtgtattat tttagttgta gaataaaact
     2341 aaatcggcaa aactagagaa atttagtgac gcaaagctat agggactaac gcttataaaa
     2401 taaactatga gttatgtcag ccagttgcca aaaagatatt gttctttttg ttttatgaaa
     2461 gttttttagg gggatagtaa tatatatgtt tagagatgaa atggataaat gtacacacat
     2521 gttaactgct tatatcagta gtttatatga ttattgtgat tttatagata cacagctaga
     2581 tgattttata ctagagtacg gagaaaatgt agtagaatct tgtttacatc aagtgatggt
     2641 attggtaagt aagtataatt aaatagaaat tgttttctag agaacccttt ttagttttat
     2701 ctacatttaa aaatgttatt atattaaaaa actaatctcc cctatatgca aagttttctt
     2761 ccatataaca atttcttccc atttatactt tttcacttgt atctttaata aaattcaact
     2821 tcaaatatta atatacttca aaaattattc tatttcttat ctttacacca attcctatca
     2881 gtattatcct ttatataggt tctaactatt tctccacaat ctaaacataa atctgaatat
     2941 atagtagatt gtactggaaa agttttattt ataactttat ctaattttcc atctcctcta
     3001 tattttaatc caatagtttc agcatatatg ccaccagttg ttaaaattcc cttttcaata
     3061 tttacacttc cacaattaga acatttcaca tttataaccc cctaaacaat tattttttca
     3121 tgctatctta taatttaaaa tataatttgc taccgatttt ataacacagt ggcaaatcaa
     3181 cattttttca ttctatttac catctaatcc attgaaatat agctatttag ttataggaac
     3241 ttgaatttcc gttacaaact cctctacaat tccagtctca tgtatatcta tcttatagat
     3301 ttctataggg tcagcaatta tcttataatt attttcttct ataaatttaa acaccttctc
     3361 catatatatc ttattattag aattactccc agtataattt aaagttacat aatatccttc
     3421 atcaaaaact atattatata ctttctcatt actttccaga aaacaaaata cagatttaaa
     3481 ctcattaaat atacctttat taattgcttc agtagaaaag gcagaaccaa tattgttgtt
     3541 tccaagtata ttaaatctat cttcatattc tttctgaagt ttttgaatta aaaaatcgaa
     3601 gtcttcatcc ctctttatat cagcatttaa ttctaaagct tttctttttt ctatatatac
     3661 aacctctatc tcatctattt ttgtattttg aattacactt tcaattgaac tcaatctttt
     3721 tataatattt tccttatggc ttataaattc agctattttt ttatcaatta aatcgagttc
     3781 ttcatttaaa atatttttcg tagactctat acttctgtca tctaggtatt cttttatttt
     3841 cttcattgga aagttcaagc ttctaaattc ttttattaaa tttaatttcc atatatctga
     3901 tatattatac attctatatc catttatatc tctaactggt cttagaatgc ctatttcttc
     3961 ataatacatt aatgaatctc tacctattcc atatatttta gatatctctc ctattttata
     4021 gtaatctttc atttatataa taaactcctc cgaaatctta tttttatttt aaatcaacat
     4081 tattactcta taatactata gtattagaat at
//
LOCUS       DAEOSP010000259         4110 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.263, whole genome shotgun sequence.
ACCESSION   DAEOSP010000259 DAEOSP010000000
VERSION     DAEOSP010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.263"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            220..1173
                     /locus_tag="KRH54_003354"
     CDS             220..1173
                     /locus_tag="KRH54_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG8580983.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            1163..2119
                     /locus_tag="KRH54_003355"
     CDS             1163..2119
                     /locus_tag="KRH54_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBG8580984.1"
                     /translation="MKLSLNQKIYFVSILLFISMALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            2116..2871
                     /locus_tag="KRH54_003356"
     CDS             2116..2871
                     /locus_tag="KRH54_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8580985.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRGLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            2963..3928
                     /locus_tag="KRH54_003357"
     CDS             2963..3928
                     /locus_tag="KRH54_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBG8580986.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAETLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         1665 a          388 c          681 g         1376 t
ORIGIN      
        1 aaaatagtat atttatacaa ataatatcta catatttaaa ttggcgttca taatttatta
       61 aattttaatg ttactagaag tttcaattct agtaacattt tttatgttaa aaacagatat
      121 agatatgtat tgacaatgat aattattatt gataaactat aaaagtgaaa atgataataa
      181 ttttcatttt aattaataaa agatttttaa ggagggagaa tgaagaaaag gtatttagtc
      241 ataggaataa taattttgtc ttatttttct ttatttatag gagctgaaga tataaatata
      301 atgcatatat ttgccaaaaa tcagcataaa cttatgatat ttatcatgag tagaatacca
      361 agattaataa gtatattgat tgctggtgta ggaatgagca tagcaggtct tataatgcaa
      421 cagataagta agaataaatt tgtatcacct acaacaggtg ctacaataga tgcagcacaa
      481 tttgggatag taatatgtat gcttttagta ccaactgcct ctatttttac aaagacaata
      541 atagcatttg tattttctct tgtagggact tttacgttta tgaaaataat agggaagtta
      601 caatttaaaa atataatctt tgtaccatta gtaggtataa tgtttggaaa tataataggg
      661 tctatgacgg attttatcgc gtataaatac gatttgagtc aaaatgttag ttcttggatg
      721 caaggtgact tttctatgat acttaaagga aattatgaga tattatatat cacaataccc
      781 cttataatat tagcatatat ttatgcaaat aaatttactg ttgttggtat gggtatggat
      841 tttgcgacaa acttaggtct tagttacaaa agaattgtca atataggtct gataatagta
      901 gcacttgtta cagtatgtgt agtcgttaca gctggtaata tcccttttat aggtctaata
      961 gttccaaata tcgtatcctt gtacatgggt gataatataa gagcaagcat atggtatact
     1021 ggtttacttg gagcgatatt tgtacttata tgtgatattt ttggaagaat tattatttat
     1081 ccatatgaaa tatctatagg gcttacagtt ggagttatag gaagtatact attcctttat
     1141 ttaatattaa ggaggaatgt gaatgaagct tagtctcaat caaaaaattt actttgtaag
     1201 tatattacta ttcatatcaa tggcactttt tataatttat ggaatagaca tgaatcattt
     1261 agaatatgca ttatcacaaa gaataccaaa aatatttgct atggtattag gtggaggatg
     1321 tatagcattt actactgtag tgtttcaaac aattacaaac aatcaaatat taacaccaag
     1381 tgtactaggt ttagattcat tgtatgtaat gattcaaact ataatagtat tcatatttgg
     1441 ttcaagcagt agtctgatta taaatgaaaa ttataatttt attattaatg tagctatgat
     1501 gattggagct tcattgttat tatataagac gttatttgaa aaaaataaaa ataatatttt
     1561 ctttttaatc ctagtaggaa tgatatttgg aaccttattt aaaagtgcaa caacatttat
     1621 tcaggtaatg atagacccta atgaattttt agctttacaa actagtataa tggcaagttt
     1681 aaacaatata aacactaatg tattgttaat agcatttata ataattattg ccataatacc
     1741 ttttatatat gatgaaataa agtatttaga tgtattgtcg ttaggtaaag aacaagcaat
     1801 taatttaggt gtagattttg ataaagcagt taaaaaaatg ataatattaa tagcaatttt
     1861 agtctcaata tcaacagcac ttattggacc tatgacattt ttaggacttt tacttgctaa
     1921 tataacaaga gaaattttta aaacatataa gcatacatac ttgatttcgg gttctatgtt
     1981 gatagggatg attacattga taataggtca attttttata caacatgtat ttaagtttga
     2041 tacaacttta agtgtagtaa taaattttat aggaggaatt tattttattc aattactatt
     2101 aaagggggca aatagatgat agagataaaa aatatattta aaagatataa gaataaaaat
     2161 gttgtagatg atgtctcttt tagtatagaa aagggaaaga taacatcttt tataggacca
     2221 aatggagctg gaaaaagtac agtactatca atagttacaa gacttatagg aggtgatggt
     2281 ggagaagtaa taatagaagg aaaaagcttg actaattata gcaataaaga attagcaaaa
     2341 aaaatagcta ttcttaaaca atctaacaat ataactttaa agcttacaat aagagaatta
     2401 gttggatttg gaagatttcc atatagtgaa ggtaatctaa ctaaagagga tgaaaattat
     2461 attgatgaag caatagaata tatgaagttg actgatatac aacataagta tttagatgaa
     2521 ttgagtggag gtcaaagaca aagagcttat atagcaatgg taatagctca agatacagag
     2581 tacatattac ttgatgaacc actgaacaat ttggatatga accattccgt acagatgatg
     2641 aaggtactta gaggtctttg tgatgaatta gataagacta tagtattagt aatgcatgat
     2701 ataaattttg catcatgtta ttcagataat atagttgcat taaaaaatgg aaaagttgaa
     2761 aaagtaggtc gtacagatga gatagtaaat gaaaaagttt tagaagatat ttatgagatg
     2821 aattttaaca ttaagaatat aaatggaaat cgaatctgta tttattttta aaatattgta
     2881 tatgaaaata aacttgctat atgatttttt ataaagtttt taaattaaaa taaagattaa
     2941 taggagagag gaaaatattg atatgaataa aaaagcagca atagtagcag cagtagctat
     3001 tataggatta gtgacagtgt ttgcattagg tggaagtaaa aagaatgaat caaaaacatc
     3061 agaaaattca aataatacta tgaagataac tcataacttg ggagaaacag atgtaaaatt
     3121 aaatcctaaa aaagtagtgg tatttgatta ttcggcttta gatacaatgg atgcattagg
     3181 tgtagctgag actttagtag gacttccaaa agcgagttta ccagcatcct tagaaaaata
     3241 taaggatgac aagtatgcag atttaggagg attaaaagaa ccagatttag aaggtataaa
     3301 gtcggcaaat ccagatttaa ttataataaa tggaagacaa gaagattttt atgagcaatt
     3361 gtcaaaaata gcaccaacaa taagtacaag taaagatgat aagaagtact tagaatctgt
     3421 taaaaataat atagacaaaa tagcaaaaat atttggagta gaagaaaaag caaatcaaga
     3481 atttagtaaa attgaaaaga aaatagaaac tttgaataaa aaagtaacag ataaaaattt
     3541 aaatgcttta actataatgg taaatgaagg taatttaagt gtatttggtg aagaatcaag
     3601 atttagtata ttatacaata gttttggatt tgaaaataaa gataaaaata taaaagaatc
     3661 aagtcatgga caaaatatta cttttgaata tatagctaaa caaaatccag aagttatgtt
     3721 cgttatagat agaggaatag ctactggcag tgatgtaaaa gaaagctcaa ctgcaaaatc
     3781 tgttttgaac aatgatataa taaaatctat ggatgcatat aagaatgata atataatata
     3841 tttagattca ccaacatggt atgtaaatga tggtggtcta acatctttaa ataaaatgat
     3901 agatgatgct tcaaaagcgg taaattaata atccgatata accaaaatgt attttttata
     3961 aaatgattat caaataaact aggagttttt aagaactcct agtttatttt ttgcataaaa
     4021 aagatatagt ttaaacaata atattatcta taataagata aatctattca aaatctaata
     4081 aaatatgaag ttatactaga caaaaagaat
//
LOCUS       DAEOSP010000260         4104 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.264, whole genome shotgun sequence.
ACCESSION   DAEOSP010000260 DAEOSP010000000
VERSION     DAEOSP010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.264"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(268..1128)
                     /locus_tag="KRH54_003358"
     CDS             complement(268..1128)
                     /locus_tag="KRH54_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBG8580987.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            1277..2167
                     /locus_tag="KRH54_003359"
     CDS             1277..2167
                     /locus_tag="KRH54_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBG8580988.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAA"
     gene            2385..>4104
                     /locus_tag="KRH54_003360"
     CDS             2385..>4104
                     /locus_tag="KRH54_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBG8580989.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKAIRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKDTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVK"
BASE COUNT         1676 a          434 c          638 g         1356 t
ORIGIN      
        1 aatatttaaa tttgtatttg attgttataa agtaaagtaa gtaataaagt tagactcaat
       61 aattctaaaa tttctaagca tactatctaa atgagaacgg atttggaaat acttgtataa
      121 aacaataatt gatacattta atttttatct taaaaacaac aattataata tataaatagc
      181 aagtataata tataaatagc aagtataata taacatataa aaagttgaat ttgtatatgt
      241 taattatact tgctatttat ttgttagtta ttctattttt ttaaatccat tacctaaagc
      301 ttcatgtata tctgtaatta taataaaagc attagggtcg acatttttta ctaatttttt
      361 caattcgaca acttctttct tttcaactac tactaaaagt acatttttat tttctccagt
      421 atatgcacct tctcctttta gaatagttgc acctctatct aaatcttcca ttattttttt
      481 acttatatct tttgaaaggt cagatataat agtgaatgat ttggaataat taaatccttc
      541 aataagcaag tcactaactt ttactaaaat gtataaagcc acagttgaat aaagtgcaat
      601 ttcaacctgt ctatttacta tacctgataa aacaactaca acgcagtcta caatacccat
      661 taaaacaggt atactaaatg taggaaaata tttatttagt aggagtgcca ttaaatcagt
      721 tccacctgta gagccattta ttctaaaaat aagtccttga ccaactccca ttaaaataga
      781 accagatata attgcaagat acatatcatt tgttatatct aatgtagcca tatttgcagt
      841 aagcttaagt gctatggtaa gaaaaattat tcctaaaacc gttttaaaac aatctttttt
      901 tgaaagtatt ttgtaagcaa atatgaataa tggaacattt aaaagtaaat tgacaatcca
      961 aagttctaca ccgaataatg aatttaaaac aactccaagt cctgttatac caccagcagc
     1021 gattgcatgt ggattaaaaa ataagttaag agaaatggcc attaatatac agcctacaat
     1081 taatattaaa atttctgaaa aagttattct atcttttttc aataacatca ctccttatat
     1141 atagattata atatatatta tatagttttt ccaattttta ataaaagaaa taaaaatata
     1201 ttataaaaac aatttgattg gaattttcag aaaattaatg ctataattaa aagtaaattt
     1261 aaagggaggt tataaaatgg ctaaattaac tctagaacat ataaaaggaa atacatatta
     1321 tattccactc cctacaatag taggtgtata cgtagatggt aaggatgcaa ttttaattga
     1381 cagtggaaat aataaggata cagctagaca agttttaaga ttattagaag aacataattt
     1441 aaatcctaaa ttaataataa acactcattc aaatgcagac catataggag gaaatgcata
     1501 tttgaagaat caaacaaaat gcaagattgc tacgactaaa attgaaggat attttacaga
     1561 aaatccaata ttagaatcag catttttata tggtggctat ccaagtaaag cactgaagaa
     1621 taaattttta ttagcaaagg aaagtgaagt tgattatatt ataccttcaa atggcaaaat
     1681 tgtagataca gaacttgaag cgatttctct tccaggtcat tattttgaaa tgattggtgt
     1741 tagaactcca gataatgttt tgtttgttgc agatagtctt acaccagaga atataattac
     1801 taaatatcat tttttcttcc tactggatat agattcacag tttaagacac tagaaaaatt
     1861 gagaatgtta gaggctgatt tctttgttcc aagtcactct gtcaaaacta cagatataaa
     1921 aagtttaatt aatattaata agaagaagat ggaagaaata atagataata ttaaaaaggt
     1981 ttgttatgag ccagttatga ttgatgaagt tattgaaaaa atgtgtgatt tgtacaatgt
     2041 caagcttgat gctaatcaat atgtgttggt tggaagtaca atacgttctt atataactta
     2101 tttatatgag aataatatgg ttgaatatat atttgatggt ggtaagatga tgataaaagc
     2161 tgcgtaaata gtaataattt tcaaattata tgatgaaatt gttttagtat gactgttatt
     2221 tgaaaagtaa tataaaaaaa tttatataaa taagctaaga tgattgatat aaagttactg
     2281 ataattttaa ggtaatatat acgttactat aatattatgt tattatttgt aaataagggg
     2341 ataatttata agcaaattaa agaccttaat ttacaggagg aatagtgtat attatgaata
     2401 aaaaattcaa aaaattcagt aaaaaactaa gtatattaat tttagttttg actatgtttc
     2461 tttttaataa tttaactgca aatgcacaga ttaaagaaga ttcaataata ggaattgata
     2521 gatatgaaac agcaggactt atagcggata gacaaactta tgatactgta atattagtta
     2581 atggagataa aaatttatct gatgggcttt catcaagtgg attagctgga gctattaacg
     2641 caccaatact tttgactaaa aaaagtgaaa taccaaaagc tactttaaat agactagata
     2701 ataagagttt aaatgttgta aaaaaagtat atataattgg tggttataat actattgaaa
     2761 attcagtaga aaaagatata aaaagaaaag gtattcaagt ggaaagaata aatggtaaca
     2821 atagaattga aactagttat aatgtagcta aaaaaataaa tgaagtaagt agtgtaaaag
     2881 aggtaatgct tacaaatgga tttgtaggag aggctgatgc tatgagtata gcacctgtag
     2941 ttgcaaaaaa taaaggagct ataatactta cagatgggaa atcagttcca tttaagactg
     3001 aaggtttaaa tgtttacgct ataggtggca aatctgcgat aaatgaggat ttagtaaatg
     3061 aaactaaagc aattagaatc ggaggaaatg atagatttga gaccaataaa aaggtaatgg
     3121 aaaagtttta taatggagct acagattttt atataacaaa tggatatcaa ttagtagatg
     3181 cactaacatt atctccttta gccaaagaaa aaccaatcgt tttagttgct gatggaagta
     3241 ataaaggtat tttaaaagga gctaaaagta taacaaaagt tggaggtatt gatgccaata
     3301 tatataaaca gtgtttagat atgattaaat ataataatat ggatatgact ccaaatattt
     3361 taaaacactt aacttcagtt gaaggaaatg aagtttctga aatacttata gaatcagata
     3421 atttaggtgt ggttgttgaa aagacgaata ccaataaatt tgagtttgat tacgtaagtg
     3481 taactaatga aaaaaattgt actttttctg tatataaaga aaattcttct aataatataa
     3541 aaaatggaaa gctagttgta agtgctaaga aaaaaataga taagaaagat agtgatttgc
     3601 aagatatgaa tggcgacaat atgataaatg caaataaaga taggatggta aatgtgataa
     3661 aaattggagt tcccgataaa atgtattcta attttaaagt agatgttgaa agtggttcag
     3721 tagaacttta taatattaaa ggaggagcta ctgttgatgt caatgacgga attgctaaca
     3781 ttgttgataa caatgtaaca aatcctttca atataaatac taatagtggg atttcaggtg
     3841 taacagctga aactatatca tctgaaataa aatttagagc aaacaatgga attgtaaatg
     3901 taacagctac aaatataaat ggcaatataa gtttatttgg aagcgataat aaagatactt
     3961 ttgatggaat atttaaatta aatctgaaaa aagaaccatc aaatttacac ttgaagttaa
     4021 ttggaaatgg gataaataaa ttgccagaag gttggtctaa agattatatt ttaggaaatg
     4081 gacaaccaat tattgaagtt aaaa
//
LOCUS       DAEOSP010000261         4075 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.265, whole genome shotgun sequence.
ACCESSION   DAEOSP010000261 DAEOSP010000000
VERSION     DAEOSP010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.265"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            162..1430
                     /locus_tag="KRH54_003361"
     CDS             162..1430
                     /locus_tag="KRH54_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580990.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     EQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGIVDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
     gene            1796..2977
                     /locus_tag="KRH54_003362"
     CDS             1796..2977
                     /locus_tag="KRH54_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG8580991.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(3119..4036)
                     /locus_tag="KRH54_003363"
     CDS             complement(3119..4036)
                     /locus_tag="KRH54_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8580992.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
BASE COUNT         1623 a          426 c          655 g         1371 t
ORIGIN      
        1 agttatagaa attttagtgt ttataatttc tatagaattt attataccct acgaatacag
       61 tgatgtattt actgttctat attcaagtat aaacatattt gtatgtagtc ttatatctaa
      121 taaacttttg aagcaaaaga ttgactttta ggagggatat tatgaaagca aaaaatatag
      181 gaaaatatac aatatgtttt ataatagtat gttttttaat gattactatg accttaatat
      241 tgagttattt taataaggat aaactatctg taaaaacttt aaaaggcaat gtaaaagata
      301 tagggagagt aagtgtagta tatacaccta atataagcaa attttcttat gaggaggtaa
      361 ttataagtaa aaatggagta agaaataata caaacccaaa atcaagaatt agaaatgatt
      421 atttaaatat tgacaaaaaa tacagtgact ttgttaatga taaaggaaaa gaatttatat
      481 ataaagatga tttgaatata ggagaagcgt atacctctgt taagtctgat ttagtacaag
      541 gaatgaatgc tttaaatgat tgtataaagg tttcaaataa aaatttaaaa agtggggtag
      601 aagaaaattt tgatattaat ttaaataagt ctgattttaa agaaaatagt tggaaaacct
      661 tatttaacaa aatatataaa ggtaatttat atatggtaat tagtgaaact aatatttcag
      721 gttctaaagt caataatatt tgtatactaa agatagatat aaaaaataag tcttataaat
      781 tattaaatag atttaattta ggattacaga atggagaaca acaaaatatc aatgaaatta
      841 aattttctat tggaagtaaa ttatacttag aaatagtaaa taataaaagt aaaaatggat
      901 atattgaagg acatgatagt tactctaact ttattatata tgatatagaa gataacactt
      961 ttaatataag taataaattt atggataatc taaacaaaga aaagaatgca aatccaaagg
     1021 agattggctt attaagtaaa actaagctag attatatggt aaaaaataat aaattgaatc
     1081 ttatagttac ggatatgagt aattccataa ctaatcttgt ttatgatatt aattctaatg
     1141 agattaaatt agaagattat gagatacttg atttaaaagt agattataat gaagcatcat
     1201 ctggagttag gaaagttaag ctactggatg ataagatata ttctatttca ggtattaaga
     1261 acagtatatc aggagaatta aaaggattta aagggattgt tgatgtagtc ggaactaagc
     1321 cagtaaaatt tcaagtattt gatatgaaac ttaaaaagaa tatttatgaa gctgagctaa
     1381 taaatggaga tataagttta caagaaaagt tattttttgt tgcaaattaa gtgttagaat
     1441 taattttaga actttaaact tttatatgta tcacagataa gtataagtaa gggagtgtct
     1501 caaaataaac tttttagctc atgagacact ttttttatat gaaatcaaat atattttcat
     1561 tgtttagatt tattttgata atgaaaaaaa gattatcttt attttaagaa agacatttat
     1621 ttataattat atacaaaaat attttttaat gtttttgtgt ataattataa caatttaatt
     1681 tatattaatg cacttaaatt tgaaatagaa tgcttatagt ggtaaaatta attattagaa
     1741 tattagaaat aataaataaa taatacattg tacttaaaag ggggagaaat ttaagatgaa
     1801 caaaaaatat attaaagcag ggaatttaat tgatggaacc ggaaatagta ttatgcaaaa
     1861 taagggtata attattgatg gagatactat agttgaaatt gaagatataa gagatgattt
     1921 aaatggatat gatgtgtacg attacttaga taaaactgtt atgccaggca ttatgaattg
     1981 tcatgttcac ttgacaatgg agccagttgg aaatccaaag atatattatg ataatgtatc
     2041 agatgtagag cttgtagtaa atacgataaa acaacttgat gcttatttgg attcaggagt
     2101 aacatatatt agaagcttag gttgtcccaa atatgtggat gtacaactta aaaatcttat
     2161 agacaaaggg tctatagatg gtcctggtat agttacttca ggtccagtta tatgtatgac
     2221 aggagggcat ggatattatt ttggtattga aagtgatgga gtagatgaat gtagaaaatc
     2281 agccagaaca gttttaaaaa atggagtaga ttgtgtaaag ataatggcaa ctggaggtgt
     2341 aacaactgat ggagttgagc cagggtcacc acaattaact ttggaggaaa tgaaggctgc
     2401 tgtagatgaa gctataaaag caggtaaaat aactgccaca catgcacaag gaagaactgg
     2461 tataaaaaat gcagtgcttg caggaattac ttctatagaa catggtgttt atttagatga
     2521 tgaaataatt gatttgatgc ttgaaagagg aacttactta gttccaactg tggctgcacc
     2581 atattttatt ctaaatgcag gaaaagaaag tggaataact gaagctactc ttagaaagtg
     2641 tgagattgtt gccattccac ataaagagtc attcctaaaa gcatataaaa aaggaattaa
     2701 aattgcagtt ggttctgatg caggaacttc ttataatgaa catggaaaaa catattatga
     2761 aatgaaacta atgtcagatt atggtatgga taatatggat ataatagttg ctgctacaaa
     2821 aacagcatct gaacttttaa gaattgataa aaattatgga actctagaaa aaggaaagaa
     2881 agcagatgtt atagttgtaa atggaaatcc agtagagaat atagacgtac ttgcagatgt
     2941 gttagcagta tttaaacttg gtaaaaaagt tagataaata gtataaaaat aaaaaagagt
     3001 gtctaaagaa atttttaaat aattatagac actctttttt attagtaata attaaatctt
     3061 ttatacttta gaaaatagat tctattttat atttagatat aactctttaa aatcattctt
     3121 atgcaatata atctatagaa tctacacata caaagtatat tcttgaatca ttagaatttc
     3181 caagtactat aacatcattt ttcttaccta gaacttcaac accagttaga accaaactac
     3241 cagctactac agtaacttga ttatttatat ttgacatata aagattatct aggatacctt
     3301 tattacaaca gcaatcatca caatcgcagt tgcaattaca ttctttgtaa tgttttttat
     3361 caagatagta acctaaagat gctatgaaat cttcataatc tgcaggtgta atatcaaatg
     3421 caacagcttt aagattacac aatgatgcta aatctacgtt tgctaatgga gtagatgcag
     3481 caagtataaa atcaattata gcagcaagta ttgcatctaa tatagcccca aggtctatcg
     3541 taggaggaat aactgcatca ataagtgcaa ttaattcagc tactcctggt atagttccta
     3601 tagtattaat taatccctca agagttaaag ggactggaat aggataagat actctaccag
     3661 ctatatctat taaatcacag ttacaagcag aaaatctttc aaaagtacca tcaagtccag
     3721 ataaattatc ttttggagga gctgaaagat ctatacctac caaattagca cctactataa
     3781 agaaatctga gataaaagca aattgattaa agtttacaaa aggtcttaga gcatcatatc
     3841 ttagaagttc taatgcttct ctcatacttt ttttacaaca acattgtttt attttaggtt
     3901 ctttatattt atttggataa ttatagtatc tatcagctac ttgataattt tcatagtatc
     3961 tttcatttgt atttggataa tcttcctcat attcttgtgt aaatctaaag tcctctctac
     4021 atttattatt ttccactaaa tacactcctt ttatatttta atttaaatgt ataga
//
LOCUS       DAEOSP010000262         3971 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.266, whole genome shotgun sequence.
ACCESSION   DAEOSP010000262 DAEOSP010000000
VERSION     DAEOSP010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.266"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            415..1671
                     /locus_tag="KRH54_003364"
     CDS             415..1671
                     /locus_tag="KRH54_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG8580993.1"
                     /translation="MIEKFKTLIWIKMIIKIKSVYKTYGGLFLLGLIGIPIGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIILGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            1734..2927
                     /locus_tag="KRH54_003365"
     CDS             1734..2927
                     /locus_tag="KRH54_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBG8580994.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIVGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKIHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGENK"
     gene            3128..3508
                     /locus_tag="KRH54_003366"
     CDS             3128..3508
                     /locus_tag="KRH54_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8580995.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
BASE COUNT         1388 a          413 c          722 g         1448 t
ORIGIN      
        1 ataggttata ttatttataa cattaaaaat acaatttaca taaaatagac atatgaaaaa
       61 taaaaaatta tataataatt atgggaatga ggttctccct tggatgataa tatcctaaac
      121 tgccaatgag ctgatgactt ctatgatatc atagatgcca ccagcttttt ttgttgttta
      181 aatcgataaa atggaggtga attacttact aaatttttat tttttaaatt tgaagaaata
      241 tttaatcaat aaaaaaaata gtagattata tgttaattat tttattaaga atgggttaag
      301 agtgtaccta tatcctatca attaaaaaaa caattacgat agtcattaca ttgactacag
      361 ttgatgcaat aaaactttca aaaaatctta tgatgcaaat agaaaggaat taaaatgata
      421 gaaaaattta aaactttaat atggattaaa atgataataa aaattaaaag cgtttataaa
      481 acatatggtg gactattttt gttaggactc ataggtatac caattggtgc aattataggt
      541 cttatagaca ccatatttgg aacggttctt ctaaaagtta cagatattcg agaaacatat
      601 ccaatgtatc tgataccatt tttagcagta gtaggagttg ttattgctta ttgctatttt
      661 aaatttggag gaaaaagtag taaaggaatg aatctaatat ttgaggtagg gcatggagaa
      721 gaagaaatta ttcctttaag attggttcca tttataatat taggtacatg gttaacccat
      781 ttatttggtg gtagtgcagg acgagaagga gttgctgttc agattggtgc aactttttca
      841 cattgggtag gtaaaaggtt gcctataaaa aatgcttcta gtatttttct agtaactggt
      901 atggcagctg gttttgcagg attatttgag acaccaatag cagcaatact atttgctatg
      961 gaggttttgg tagctggttc actggagtat caatcgcttt ttcctgcgtt tactgcttct
     1021 tttacagcaa gtgcagtatc aaaagcttta gggttagaga aattttcttt tgcactttct
     1081 tctaaagttg tatttgactt gtcaatattt tggaagctta ttgttttagg aattattttt
     1141 ggaatggttg gtggagcatt tgcatggtgt ctgaaattgt caaaaagaaa aatagggaat
     1201 agattaaaaa atcctatgat aagaattgct attattggag tatgtcttag tgtattattc
     1261 cttttatttt ataaaggaag atattcaggt ttagggacaa atttaattca aaatagtttc
     1321 tatggtggag aaatttattc atttgactgg ctgttaaagt ttatacttac aatactgact
     1381 ttgtcagcag gttttcaagg aggagaggtt acacctttgt tttccattgg agctagtctt
     1441 ggtgtattgt tagcaggatt ttttaatttg ccaattgaat tagttgcagc gcttggctat
     1501 gctagtgtct ttggtagtgc tacaaatact ttttttgcac ctgtatttat aggggcagaa
     1561 gtttttggat acagctattt accttatttc tttgtagtat gcgctatttc atatatattt
     1621 aatatggata aatctattta ttcactacaa aaaatttcaa caaaacaata aataaaacaa
     1681 cataatgtat gattagaaga gaaagaatat tagagaaagg atagggtagg aatatgttga
     1741 caagtttagc tttgattttt ttattaggaa tggcctcggg aggtattttt aagaggataa
     1801 agttacctaa cttattggga atgttactta caggaattat attaggtcca tatgttttaa
     1861 atttgattga taattcaata ctagatattt cttctgacct tagaaagatt gcattgatta
     1921 ttatattaac aagggctgga ttatctttag atataaacga tttgaagaaa gtgggaagac
     1981 ctgctgtact tatgtgcttt attccagcta cttttgaaat tgtcggaatg atagttttag
     2041 caccaaagtt actaggcgtt tctatattgg aagcagcagt catgggtgca gtagtaggag
     2101 cagtttctcc cgcaattatt gtacctaaaa tgcttaaatt aatggaagaa ggttatggta
     2161 cagaaaaaag tattccacag atgcttttag caggaacttc tatagacgat attttcgtaa
     2221 ttgtgatgtt tacagtattc acaggattgg cacaaggaaa tagtatatca gctattagtt
     2281 ttttacaaat tccagtatca attatattag gtgtaatagt tggagcagtg attggaatct
     2341 gtttagcagt attttttaag aagattcata tgagagattc tgctaaagga gtgttgcttt
     2401 taagtatctc atttttaatg ataagtttag agactgcttt agagggtatt gtgccatttt
     2461 ctggattatt agcagttatg agtattggga ttttccttca gataaaatat cgtgtagttg
     2521 caagaagatt gtctataaag tattcgaaac tatgggttgg tgctgaaatt ctcttgtttg
     2581 ttttggtagg ggcaacagta gatataagtt atgcatttaa agcaggaatt ggagctgtaa
     2641 ttttaatatt tggagttctt ttattccgta tggtaggagt attcttctgc cttattaaga
     2701 caaatttgac aataaaggaa agattgttct gtatgattgg ctatattcca aaggctacag
     2761 tacaggcagc aataggaggt gtaccacttg caatggggat ggcatctgga caacttattt
     2821 tgacgttagc agttctagct atacttataa ctgcacctct tggagctttt ggaattgatg
     2881 taacttataa aaaattatta acttctgtaa aaggcgagaa taagtaaaaa taaagattat
     2941 tttaactggt aagttatatt ttggaaaagg attatactat tgaatacaat ataaaaaaat
     3001 catttgggag attaagaagg tagaagttaa gaacagattt taaggtatgt tatgattatt
     3061 agaattgatt ttgtgaaatt attattgtaa tatttaatta tatagataaa gaagcggagt
     3121 gataactatg tgtttaaaaa aattatcaaa attagagtta gttattatga aatttatttg
     3181 gaatttagat ataaaaacca attcatatga gattattgat tatatgaaag aagaacataa
     3241 tttaccagaa aaagtagctt taaaaacttt atcaaaacta tcaaagaaaa gattcttata
     3301 tgttcaagaa acaggcaagt gtatgtatta tacagttgca ataaaagaaa aggcatattt
     3361 agagtttata tcaagaaatg tgcaaaatct tttgaagaat aattttataa ggaatttatt
     3421 agtatcattt cacgaagagg agctaacaga ggaaaagata aagtcgttag aaaactgggt
     3481 agtaaattgg gaagaagctt atgtataaat gttattattt ttattacaat ttagataatt
     3541 ttggttttgt aagctctgca actattttta gatggttgca gagtttattt ttatttttta
     3601 ttcccttaag caatacttaa aagtaaacgc atttatattt actaacttca attacttata
     3661 atacttaaat tactttttaa aaatattttg atatgtcatc tctatagtaa tctagaactt
     3721 gtttctattc tttatataaa gtttttatga atgatatata taatattcgt tcatttgatt
     3781 aaaaataaca aaatattaaa taattctact ctgatagttt gttaaaaaaa taataaaaaa
     3841 tattaataaa caaaaaaaaa ttatcttaag agaggagaat gttctaaaat ataaaaaggt
     3901 ttctagattt cataaaagat actattttag tcttgaaaat atttagtttg aaaagatttt
     3961 aatttaatga t
//
LOCUS       DAEOSP010000263         3958 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.267, whole genome shotgun sequence.
ACCESSION   DAEOSP010000263 DAEOSP010000000
VERSION     DAEOSP010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.267"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            65..268
                     /locus_tag="KRH54_003367"
     CDS             65..268
                     /locus_tag="KRH54_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8580996.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKSI"
     gene            432..1586
                     /locus_tag="KRH54_003368"
     CDS             432..1586
                     /locus_tag="KRH54_003368"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBG8580997.1"
                     /translation="MKKISILGSTGSIGKQTLDVVRDNRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            1599..2603
                     /gene="rseP"
                     /locus_tag="KRH54_003369"
     CDS             1599..2603
                     /gene="rseP"
                     /locus_tag="KRH54_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBG8580998.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            2785..3840
                     /gene="ispG"
                     /locus_tag="KRH54_003370"
                     /gene_synonym="gcpE"
     CDS             2785..3840
                     /gene="ispG"
                     /locus_tag="KRH54_003370"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBG8580999.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
BASE COUNT         1537 a          394 c          777 g         1250 t
ORIGIN      
        1 taaatagatg aaaattttcc aaaatagata aaatgtgata gaatatattt tgcgaggtga
       61 taacatgatt tcatacgctc cattatggaa attgttaatt gacagaaaaa taaagaaaat
      121 ggaatttgta aatatatcag gtataagtat ttctgtttta ggcagactag gaaatgataa
      181 atctgtttct atggatacaa tggagaagat atgtttagca ctagactgta aaatagaaga
      241 tgttgtggaa ataaaaaaga gcatttaata atactagaat attttataat attccacaaa
      301 atacaaaaaa taatagatta attttaatct gtggaataat atatgaaatt tctatgaata
      361 gtaaaaataa ttgttttagt agtgttataa tgttttgaca gggtatattt attattagaa
      421 aggattatta tatgaaaaag atatcaattt taggttctac aggctctata ggtaaacaaa
      481 ctttggatgt agttagagat aatagagata agtttgagat agttgcaata tcagctaaca
      541 gtaatattga attattgcta gaacagatag tagagtttaa acctaaatat gtaacagttt
      601 ttgaggaaaa taaagcattg aaattaaaag aaatgttgcc taaaaatata gaaatagaag
      661 ttttagcagg aatggaagga ttaaaaataa tatcatcact tgatgaagtg gatgtccttt
      721 taactgctgt agtaggtatg ataggattag tcccaactct ttgtgctata aagaagggaa
      781 tagacatagc attagcgaat aaagaaacgt tggtaactgc tggtgaactt gtaatgaaag
      841 aagcagaaaa atacaatgta aatattcttc ctgttgatag tgaacatagt gctatatttc
      901 aatgtctaaa tggagaaaat aaaaaaaata tagaaaagat aatacttaca gcatctggtg
      961 gtccatttag aggaaagaaa aagggagaac ttgcaaatat aactaaaaat gaagctttga
     1021 agcatccaaa ttggagtatg ggaagaaaaa taagtattga ttcttcaacg cttatgaata
     1081 aaggacttga agtgatagaa gctagatggt tatttggagt agaacaagaa aatatagatg
     1141 tagtagttca cccacaaagc ataattcatt caatggtgca gtatacagat agctcaataa
     1201 ttgctcaatt aggatgtcca gatatgagac taccaataca gtatgctctt acatatccag
     1261 atagaatgga aagtagcttt gaaagaatga atttttcaaa atttagtact ttaacttttg
     1321 aagaaccaga tttagaaact tttccatgtt taaaattggc atatgagtgt ttaaaaatgg
     1381 gaggaactta ttcctctgtt ttaaattcag caaatgaagt tttagtaagt gaatttttag
     1441 aggataaaat tggtttctat gacataccat attacataga gaagacttta gaagttcata
     1501 gtagcataag tgaaccaaca ttagaagaaa tcttagaaac agatagatgg agtagagctt
     1561 atgttgcaaa tctgataaaa aaataggaag gtgaatttat gactataata gccgcattaa
     1621 tactatttag tataattgta ttaatacatg agttaggaca ttttatattt gcaaaaagaa
     1681 gtggtatcaa agtaaatgag ttttctatag gtatggggcc taagatatat agtgtaaaaa
     1741 aagatacaga gtattctata agagcacttc caattggtgg atatgtcagc atggaaggtg
     1801 aagatgaaga acaaataagt ccaaattctt ttggaaacaa gtctatatta caaagattta
     1861 gtactattgt agcaggacca atattcaata taatattagc agcaatactt ttagttccag
     1921 tatttttgta tataggttct ccaacgacta aattaggaaa gataatgcca gatactcctg
     1981 cacaggctgt agggcttcaa gtaggagata agataaataa aattaatggt aattctgtta
     2041 agacttggga tgaagttgca aatataataa atacttcttc tggtggagaa ttaaaactta
     2101 gtataactag agatggaagt gataaagttg taaatgtaac acctaaaaat aacaatggaa
     2161 agtatgaaat agggattcaa ccacaaagag aaaaagactt tttggggtca atagtaaatg
     2221 catgtaaaac tactgtagat atgacaaaac agatgctaac atttctaggt caaatgatta
     2281 caggacgtgt accaggaggg attggcaatg ctgtagcagg accagtaggt gttattggta
     2341 tggtatcaga tgctgctcgt acaggcatca taaatgtagt gtatttagca gctgtcataa
     2401 gcttaaattt aggtatagta aatctattac caataccagc acttgatgga tggagaatac
     2461 ttatgctgtt actagaagct gttagaggtg gtaaaaaact cgacccaaat aaagaaggca
     2521 tgataaatgt tgttggtttt ggagctttaa tgttatttat gctttttatc acatataaag
     2581 atatactaag attatttcaa tgatttagtt tttagagatt ttaagaaaat aggctatgta
     2641 gttttaaata agaataaatt aatttttata tatgaaattt attcttattt aactagatag
     2701 ctttttaatt tataatatac atgtaggtta taatatttta atatttaaag agattcaata
     2761 taaaataata tatagaggtg gaatatgagt tacgagagaa gaaagacgag agaagtaagt
     2821 gtaggaactg taaaaatagg aggagaaaat cctataagta tacaatctat gacaaatact
     2881 gatacaagag atgcagatgc tacaatagct caaataaaga gattagagga agctggttgt
     2941 gatatagtta gagttgctgt tcctgatatt aaagctgcta aaaatatagc aaagataaag
     3001 tctagtgtaa atataccaat tattgcagat atacatttcg attataagct agcccttgaa
     3061 gctatagaac aaggtgtgga tggaataaga ataaatcctg gtaatattgg aagtatagag
     3121 agagttaaga tggtagttga gaaatgcaag gaaagaaatt taaaaattag aattggagtt
     3181 aatggaggtt ctttagagaa agaactttta aaaaagtatg gctctgcaac tgctgaagca
     3241 ttagttgaaa gtgcaatggg acatattaag attcttgaag acttagattt tcataatata
     3301 gttatatctc taaaatcatc cgatatttat aaaactgttg atgcatatga attaatatca
     3361 aaaaaagttg attatcctct tcatattgga attacagaat ctggaagtgt tcacaaagga
     3421 acaataaagt cttctatagg ggtaggagca cttcttctta aaggaattgg agatactgtt
     3481 agaatatctt taacaggcga ccctgttgaa gaggttattg ttggtaaaca gattttaaga
     3541 agtttgggac ttttaaatga taagattaaa gttatatcat gccctacatg tggtagatgt
     3601 aatatagact taattagtgt tgtaaatgag gttgaagaaa agattggtag tatggaaaaa
     3661 aatatcactg ttgcaattat gggctgtgct gtaaatggac ctggtgaagc cagagaggca
     3721 gatataggta ttgctggggg taaaggtgaa ggtcttttat ttaaaaaggg tgaaatagtt
     3781 agaatgattg atggtaacaa attagttgat gagttgctag aagagattga aaaattatag
     3841 tatacaattc atatatatat taattgacta gatttttaaa tacttttgta ataagttttt
     3901 atttacagta tttaattatc tagtcatttt ttattttata aacttcatta aatgtaag
//
LOCUS       DAEOSP010000264         3926 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.268, whole genome shotgun sequence.
ACCESSION   DAEOSP010000264 DAEOSP010000000
VERSION     DAEOSP010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.268"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..596
                     /locus_tag="KRH54_003371"
     CDS             <1..596
                     /locus_tag="KRH54_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBG8581000.1"
                     /translation="IIAVIFIISVTMLILISHINILIIADIKNKDICLKLNIKYMFNL
                     ININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKIKIHELYSNINFG
                     TGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGNIRIHIRPRIKAL
                     FNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            550..978
                     /gene="ytfJ"
                     /locus_tag="KRH54_003372"
     CDS             550..978
                     /gene="ytfJ"
                     /locus_tag="KRH54_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBG8581001.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1226..1666
                     /locus_tag="KRH54_003373"
     CDS             1226..1666
                     /locus_tag="KRH54_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8581002.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            1682..3133
                     /locus_tag="KRH54_003374"
     CDS             1682..3133
                     /locus_tag="KRH54_003374"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBG8581003.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVFTTFDKINMISRNFNS
                     KENSIDTVTLIENNNLVNVCFVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3224..3892)
                     /locus_tag="KRH54_003375"
     CDS             complement(3224..3892)
                     /locus_tag="KRH54_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBG8581004.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT         1641 a          354 c          672 g         1259 t
ORIGIN      
        1 taataatagc tgtaattttt attataagtg taactatgtt gattttaata tctcatataa
       61 acatcttaat aatagctgat ataaaaaaca aagatatttg tttaaagctt aatataaaat
      121 acatgtttaa tttaataaat ataaatagac aactttatcc agctgaaaat agtaaaaata
      181 atgataaaaa agaggggatg aagaataata tagattcaag tatattatta gctgatgacc
      241 ttttgagcat ttatagatta ttaaaaaaga taaaaataca tgaactttat tcaaatatta
      301 attttggaac tggaaatatt ggcttaacat cttctgtata tgtattgata aatactttgt
      361 atggaaattt atttaatatg attgatgcag aaaaaatgta tttaaatgta aatccagatt
      421 ttacaaaaga ttatgtttta ggaaatataa ggatacatat aaggccaaga ataaaagctt
      481 tatttaatat tataataatg attaataaaa ttatgaataa aaataaagga aataaggaag
      541 gtgatagtaa tgagagcaac aggttcgata cagagtctta tggaaacaac tcttgaaaca
      601 attaaaggtt ctatagatgc aaatactatt ataggagacc ctataaaaac agatactaca
      661 gtggttgtac ctatatcaaa ggttacaatt ggctttggta taggaggagg agaatactca
      721 aaaggatatg atgataaaga tagggaaatt gaattaaaaa atgaaaaatc agatacgaat
      781 tttgctggag gtagcgcagg ggctatatct gtacaaccag tagcatttgt agtagttgaa
      841 agtggagaga ctagaataat gagtttagat agtaatatca atctagtgga taatatatta
      901 tctataactc ctagagtatt ggagaaaatt caaaatatat cacaaagtaa taagaatcaa
      961 gataaaaaca atatgtaaga ataaaatatt tttaaatatg tatatagttt taagtaaagt
     1021 agtggggtta atataaatcc cactattttg ttgtgtactt taaacattta tatctaataa
     1081 gattttagtt taaaacaatt taaatagata ataaagttga tatatagtat aattataaaa
     1141 agaaaagaag tattatatta tagatatata aacgcatttg agatataaga gggagttata
     1201 aatcattagt tttgaggtga atgttatgag cgataaagaa ttatctattt tgttgttaga
     1261 aagtatgaat aattttcata atttaataaa gattattaat aacgaaagat ataaaaaaga
     1321 taaggttctt acagaaagac agttctttgc cttagtaaaa ataagaaaac atgataaaat
     1381 agagcttaaa aatctaagta gagatttgca tgtatctact tctagtctgt gtatactact
     1441 taataaattg gttgagcaag agtatgtata tagggaagaa gatagtagag atagacgaaa
     1501 tactttttat ggaataacta aaaatggaga aaagatattg gataatgaaa tactaaagtt
     1561 tgtatctata ataagtgata aaatggattg cctagatata gataataaag ataaattatt
     1621 tacttctttg gaagagtcta aaaatataat agaacaatta ttttaaaaag gagaatttat
     1681 tatgaatata aagtattcta aagagatatt aaaaagttta gaaaatgcta taacagtagt
     1741 agctctattt gagggagaag atgtttttac gacatttgat aaaataaata tgataagcag
     1801 aaattttaat tctaaagaaa atagtataga tactgtaaca ttaattgaaa ataacaattt
     1861 ggtaaatgtt tgttttgttg gttttggtaa gagagagtta ttgaatagag aaaaaattag
     1921 aatcataggt gggaatttgt gtaaaaaatt aaaaaatata ttgaaaaatg aagcatataa
     1981 gagtagtgat gttaatgtac tgaatcttaa tttaaatagt gatgaaatag gttgttttgt
     2041 agaagggata ttacttggaa attataagtt tgataagtat aagacaaaaa gtaaggattt
     2101 tgaaagaaaa gaagttgata ctataactat atttactgaa aaagaagttg agtgtcaaat
     2161 agaaaaggct aaaatattag ctaattcaac tataatagct agaaatctag ttaatgaacc
     2221 atcaaacgta atatatccaa aaacattagc agtagaaaca gttaaacttg gtgcagagtt
     2281 tgggtttaat gttgatgttt atgaagaaga aaaaataaag gcattgggaa tggatgcttt
     2341 tttcgctgtt tcaagaggtt caattaataa accaagattc atagtaatga gatactttgg
     2401 agataaagaa agtacagata tattaggtct agtaggtaaa ggattgactt atgatactgg
     2461 tggatattct ttaaaatcaa atgcttctat gctagatatg aagacagata tggctggtgc
     2521 tgcgagtgtt ataggtgcta tgtgtgctat atctcaaagt aaattaaaga aaaatgttat
     2581 agcagtagtg gctgcatgtg agaatgcttt atcaggaggt tcatacaaac ctggagatat
     2641 aattagttct atggcaaaga aaactattga agttttaaat acggatgcag aaggtagact
     2701 tacattagct gatgcaatat actacattat aaacaatgaa aaagtaacaa aggtggttga
     2761 tgtagctaca ttaactggtg ctgctttaac attgttaggt aatgttgcaa ctcctatagt
     2821 aacaaataat gatgattttt attgtgaatt agaaaaagct gccactttat caggggaaag
     2881 ggtatggaag atgccaattt atgatgaatt taaagatatg ataaaaggag aagaagcaga
     2941 ccttaaaaac actggtggta aaaatgctgg atgtataaca gcaggagctt ttattggtga
     3001 atttgttggg aatactcctt ggatacatat ggatatagcg gggacaagta catcatcaaa
     3061 atcaattgga tacaaagcaa agggggctac aggagaacct gttagaacat tgtactattt
     3121 ggcagaaatc taaaaaacaa ttataaaata taatttataa agccacgtgg atatttttgt
     3181 aatttaagat tatctatgtg gctttatttt ttatttaagt aaattattta ctctttttct
     3241 ttttttcaag tataaaagta tctctattaa ataaaagagc aggtatgtac ataatactag
     3301 taacaaaaat aaacgtaaaa atagcagttg tgctatgact taaaaaacca taatagtacc
     3361 tagagttggt aaatgtgttt agtaaaaata tgaatgaaaa ataaataaaa catatagcta
     3421 agttatctct aagtgcatat ctgttaactc taaattttct aacatcaacc atatatatat
     3481 ctgcataaat tatcaagcac ttagagaaga taaagataaa atatattaag ctaggaaatt
     3541 catagcccaa gtttttagaa aaaatgagtt ctccaactga acaaactaaa ctccaagaaa
     3601 aaaatatatt aaaaagatga tactgcttaa aaattagtat ggctatacac agatatatac
     3661 aaatataatt gattccaatt ggtaaagagt ttagagtact ataattacct gaagaaataa
     3721 gtaataattg ctctagagcc ataattatta caagtaatgt acaaattatc ttctcaactg
     3781 tatagctata aggtaaaata tttttggtta gttttggaca atagtataaa aataatccaa
     3841 atataaagag acttacaaat tgttcactag aaaaaagaaa aattttaaac atataacatt
     3901 ctctcctata ataaatttaa tatgta
//
LOCUS       DAEOSP010000265         3884 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.269, whole genome shotgun sequence.
ACCESSION   DAEOSP010000265 DAEOSP010000000
VERSION     DAEOSP010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.269"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(70..1158)
                     /locus_tag="KRH54_003376"
     CDS             complement(70..1158)
                     /locus_tag="KRH54_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581005.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            complement(1443..2141)
                     /locus_tag="KRH54_003377"
     CDS             complement(1443..2141)
                     /locus_tag="KRH54_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBG8581006.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            complement(2255..3760)
                     /locus_tag="KRH54_003378"
     CDS             complement(2255..3760)
                     /locus_tag="KRH54_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG8581007.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
BASE COUNT         1344 a          635 c          389 g         1516 t
ORIGIN      
        1 actattttga ctttttaaat tcggattata taaaaaattt ctttaaataa aaactattaa
       61 taagtggctc tattcctgac tttgtgttga tttttttcta catgtaattg ctttatacac
      121 catacagact aaaattaaaa gccccatata acctgtatat ggatataaaa tgtttactaa
      181 tttaccaaac ggtaaaaaac ctcctataca agccaaaata gctattactg ttgttaccaa
      241 tttaaattta ggatgttcat cttcaacaat tctattggtt acagaccata aaagtggaac
      301 agctgttgta tagataccta atattaatgc aactgaaaaa catacaccta caatcggaga
      361 tatattatct gctaaataaa gtgatggtat ttctttcata tacaaatttc ctatatctga
      421 aatcatagcc aaatacataa ctataccagc agtcataaac actattccac ctaataaacc
      481 tccccaaaaa gcatcctttt tactttcaat agaagctccc attcctgtta aaaaaacaat
      541 cattacaata atatttaatg tattatatat tacaccagaa acgattggac tttctgatgc
      601 ttttgtaact tcaatatttt tcatagcttc tccaaccata gataaaccgt caaaatgctt
      661 aaacaagctt attaatccaa cagcaattgt aaatactgct atcactggtc caatacgtcc
      721 aagtatgctt actaattttt ctaaacccat agacactgta attaaagtag caacagccat
      781 caatactctt cctaagtaag gatttatacc atagtattca gttaaagttg agcctgcacc
      841 tgaaatcatt actataagaa tcccaaataa aaatattgga ctaaaccatt cgtaaaattt
      901 tcctaaaatc ttcccacaat aaaaagtgta aattcttgtt ggttctttaa gttttaattc
      961 tctacctcta gttataactt cagcaccaag aaatgaaaac aataccattg atattaatgc
     1021 tccaattaaa cccatatacc cttgtgaggc aaaaaattgt agaatttctt gaccagtagc
     1081 aaacccagaa ccaattgctg ttgctagata agcgcctgca atacagataa tatttttcat
     1141 attcactttg tctgacatat taaattacct ccattataaa gttaccttcc tttaagaaat
     1201 ataaactctc agtgaattaa atgaaaattc tgtattttta aaacagtatt taattcactt
     1261 tttttatttt gtatatcttt ttctttttat ttatatatat ttttttatat aaataggtac
     1321 agcttttata aggtttaata ttattaaata tgtttaaagc gctttaattt ttatcataat
     1381 taaagtatac ttattcagac acactcaaac aattatctat attgactttt tatttatcaa
     1441 tttcaatttt tagtgttcag tttataaact tttggagtca ttcctttttc tatcttaaag
     1501 tttttataaa agttatttag gctattatat ccaactattt ctgcaatctc atttatactt
     1561 tttgtagtat tttcaagtaa ctcaatagat ttttctattc ttatatgatt tagatatgat
     1621 ataaaagttg tatttaaatt acttttaaaa atcctagata tattagatgg attggttaaa
     1681 aataatctag cacaatcatt taactttaag tcattgctat agtttagatt tatatattcc
     1741 agtatctcat ttaataaatc attattggta tattgatatc caataatttt ttctacagac
     1801 ttagtaaatt ctttcatatc caatggttta aggagtatat cttttacatt taatctcaaa
     1861 gctttttgag catattcaaa atagttaaat gcagttatta ctacaaagtt tattttttta
     1921 gataaatgag atgttttttc tattacttct aacccgttgt atattggcat ttgaatatct
     1981 ataaaaacta tgtctggctc taaaccaatg attttatcta ctgcttcatc accagatgtt
     2041 gctttaccaa caatttttat tggcatatct ttcatttcta taaaatgtgt tattattttt
     2101 agaacagctt cttcatcatc tattattagt gctcttctca tgagaatacc tcccttttag
     2161 cttaataata taaaaaagta agatatataa aacaacataa aaacatctaa tatatttctt
     2221 aacatttata aaacttatta atttttcaca agttctatac atattcctta taattttcaa
     2281 taggaatttt aatcattatg taagttcctt cattatcact cgactttata tccatatcaa
     2341 aattatttcc ataagcagat gtgaattttg tatatattaa agacaaacca tgaccattat
     2401 tactacgaat acctgccttt attttgtcta tattatttcc atctaagata gttccattat
     2461 tatgaatctc tataatagca tactgagaat ctaatcttgc aattatttca attcgctttt
     2521 ctgtgtcaat atctctaaat ccatgtgtaa atgcattctc tacaataggt tgaataaagt
     2581 taaaaggcac agaaaggtta tataacttct ctggaataag atattttact ttaagtgcat
     2641 ctccataacg taatttctgt aaattcaaat aatttttaat gtataatatt tcagattcta
     2701 attctacaaa tcttaaatca gacctcattg tatatctaaa cattcttgat aaatctatta
     2761 ttgcactata caaatcataa ctaccatcat ctagcgctat gcttgccatt gaatttaatg
     2821 tattaaataa aaagtgatga tttattctta aattagtaac catatcttga gctactttta
     2881 aattcatttc aagttgctct ccataatgat atgttcttgc tatagtcttc tctttttctt
     2941 gtaaatcctt tcttatatca ttaaaagagc aaaaatttat tatattttta gatatttgtt
     3001 tcaggaggcg ttttatactt ctagcagctc cctctggaat atcatataaa ttattatgct
     3061 ctgtatctaa attcaaatca gataatagta cataccctcc tcttataaca cctatgctat
     3121 tgcttttgta cagtattggt atattatata tagttatccc atattcacat acaaattgat
     3181 tgctactttt ttgattcata caatcacaat tttgaggaaa tttcatagga ttacattttt
     3241 caaaacaaaa aagtggaaaa taaggatttt ttaaaactat attccatgaa tcatcatata
     3301 taattaatgg aattttaaac gggtctataa attgaccacg aattgattct actgctgcat
     3361 acaaatttaa tgtactttca ctataattta tcaaggtctt ctttaaataa ttattaggat
     3421 taatatctat aacttcactc tctcctacat catctacatt atttgaaaca ataagttctt
     3481 ttatttctct atcacctgta tttatagttt catgtgtgct accagattct atataaaaaa
     3541 tcataccttg ataaaatggc ttccaaactc catttataat atacaaaccc tctccctcta
     3601 aaatatatat catctgctct tgaccataat gaacatgatt aaaatgtttc ttaccaggca
     3661 tagttactgc aatgtatata ttcatagctt gttttggatt attttcatca taagtatgtt
     3721 tccattcaat atatccccac tcagtatttt gaattttcat aaaagattcc tttcctgtaa
     3781 attatttatg ttcatattgt gaaaaattag taaaaaaaat gtgtacatac tctttcatat
     3841 gatagcttac tatatcaata ttcattagtt tgtacacatt ttta
//
LOCUS       DAEOSP010000266         3803 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.270, whole genome shotgun sequence.
ACCESSION   DAEOSP010000266 DAEOSP010000000
VERSION     DAEOSP010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.270"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            52..960
                     /gene="trxB"
                     /locus_tag="KRH54_003379"
     CDS             52..960
                     /gene="trxB"
                     /locus_tag="KRH54_003379"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBG8581008.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1050..2873)
                     /gene="typA"
                     /locus_tag="KRH54_003380"
     CDS             complement(1050..2873)
                     /gene="typA"
                     /locus_tag="KRH54_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBG8581009.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3085..3327
                     /locus_tag="KRH54_003381"
     CDS             3085..3327
                     /locus_tag="KRH54_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581010.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3475..3666
                     /locus_tag="KRH54_003382"
     CDS             3475..3666
                     /locus_tag="KRH54_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBG8581011.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
BASE COUNT         1367 a          522 c          616 g         1298 t
ORIGIN      
        1 aatgatgaga ataatatata agaaataata atataaagag aggtaaacgt tatggtagat
       61 atcattgtaa ttggtgctgg accagcaggt ttaacttcag caatttatgc tatgagagca
      121 gggttaagcg ttacagtatt tgaaaaaaat atttatggag gtcaagttgc aagtacctct
      181 gaggtagaaa attatccagc agttcaaaag atttctgggg tagaattttc taataatata
      241 tacaatcaag cagttgctca aggtgttgat attcagtttg atgaggttga agagataaat
      301 ttagagggaa aagtaaaggt tgtaaaaact tcttcaggtg aacataaagc aaaggctgtt
      361 attcttgcaa atggagttga aagacgtaaa ttaggttgtg caggggaaca agaatttaca
      421 ggtagaggag tatcttactg tgcaacatgt gatggagcct tttttaaaga taaagaagtt
      481 gctatagtag gtggaggaaa tactgcacta gaagatgctt tatttttagc taataattgt
      541 acaaaagtgt atttaattca cagaagagat agttttagag gtgaagaagt gttggagaaa
      601 tctgtgaaag caagagagaa tattgagata ctttacagtc atggtgtaga aaaaatagaa
      661 ggagaaaaga ctgtatcgaa aatagaagta aaaaacttaa agactgaaga aaaaagaact
      721 atagatgtta gtggtatttt tattgctata ggtcttaaac ctaataataa aatgtttgaa
      781 aatgttcttg atttggatga aggtggatat ataatttcag atgagtcttg tacaacatca
      841 gtagaaggtg tatatgtagc aggagatagt agaactaagt ttttacgtca aataataaca
      901 gcagcatcag atggagctat tgcagccgtt caagcagcta attatataaa tgttgaataa
      961 tataaatttc aaataaaaat agccatataa actgaaataa tttttcagta ctatatggct
     1021 attttgttaa ttagaggtaa atgtacgttt tatttacctt gtctagattt gttatatctt
     1081 aatctatcat gttcatttaa gaatcttttt ctaagtctta tagaatcagg tgtaatttca
     1141 actaattcat catcttctat aaactctagt gcttcttcaa gagtaaatat tctaggagga
     1201 gataatttta tagcatcatc tgaaccagaa gcacgtacgt tactcatttt cttgttttta
     1261 caaggattga ctaccatatc atcttttctg gaattcatac caattatcat accttcgtaa
     1321 acttctacac cagggtctac aaacattaca gctctatcac ttaaggcatt aagagaatac
     1381 cccatagtaa caccaggtcc ttgtgctatt aaaacaccat ttcctcttga aggtatttct
     1441 cctttaaatt cttcaaattt ttcaaatgaa cgaactaaag taccttctcc acgagtagcg
     1501 tttataaatt cacttcgata tcctaaaaga ccacgagtag gtgctaagaa ctctattttt
     1561 acatagtctc cttctattga catagactcc atcatacctt ttctcatgtt taattcattt
     1621 ataatagtac cagagtaaac ttcaggacag tttacaacaa ctctttctat tggttccatt
     1681 agttttccat cttctttatg cattaaaact tcaggtttag aaacaccaac ttcataacct
     1741 tcacgtctca tattttcaag tagtatagat aagtgaagct ctccacgtcc agaaactttg
     1801 tatccatcag tagtatcaag aggttcaact ttaagaccaa catttacttc aagttccttt
     1861 tcaagtctat ctttcaaatg tctagttgta acgaattttc cactttttcc aacgaatgga
     1921 gagtcgttta ctaagaagtt catagaaaga gtaggctctt ctatttttat catttccata
     1981 ggtagtggac tatctaaatc gcatatagtt tcacctatag atatatcagg aataccagct
     2041 ataacaacta tttctccact agtagcttca tcaacttcaa cttgttttaa accttcatac
     2101 acagaaagtt ttgatacttt tcctttagat acaacagaat cttctctaca tatagcaact
     2161 tgtgtattgt ttttaagagt acctttgtaa attctaccta taccaagtct acctacataa
     2221 tcatcatatg caagagctga tatttggaac tgtagaggtt cattatcgta attaggataa
     2281 gcttcaacgt gatttacaat agttttaaat agaggagaaa ggtcttcact atcatcatcc
     2341 atttcaagtt tagcaatacc ttgtttagca ataccatata ttataggaaa ttcgcattgc
     2401 tcatctgttg catttaagtc tacaaataaa tcgaaaactt cattaactac ttcttccgct
     2461 ctctggtctt ttttatcaat cttgtttata aataatattg gttttaaacc gaactctaaa
     2521 gatttttgta aaacgaatct agtttgaggc attgggcctt cactagcatc aactaataga
     2581 ataactgtgt caacagtttt cataacacgt tcaacttcag aagaaaagtc actgtgtcct
     2641 ggagtatcaa ctatgtttat tttgtaatct tcatagttta ttgcacagtt ttttgaatat
     2701 atagttatgc ctctttcttt ttcaaggtcg ttactgtcca taacgcagtc tttaactact
     2761 tcgttctttc taaaaacacc gctttgagaa aggaaagcat caaccaatgt tgattttcca
     2821 gcgtcaacgt gggctataac tgcgatattt ataattttat gtttttgtga catttttgtt
     2881 gctcccttcg attaaaataa cttattaatt atagcacaat atattattag gtacaaggag
     2941 gaatatatta aattgtatta atatagttta aattgaataa aactgttagt tttgcatctg
     3001 aaaattattt tgtttaaaaa aattttttag acaaataata accttaatag ataaaaagat
     3061 taataaaaga gtaaaggatg atatatgttg gaagtaacca aaataataat taaatatttt
     3121 gcaagtgaaa gtgatacttt tgtaaaagaa gaagtttgta attttgaaga acttcttaag
     3181 tggtctcata aatttattga aggtaaaaag atagaagatg ttttgtatat aaagtctaaa
     3241 gaaaactata aagtaaatgt gattaagtct ttagaaatta aggaattgga aatagtgaga
     3301 aaacaacaga taccaccgta catgtagtat taataatata taaaattaat atgtaatact
     3361 cgcaatagga tataaatggt atatatggta ataatttata ttaaaaaaac ttttataaag
     3421 tttatatttg ggtataaaag tattaattag acattaatta ggaggaactt aaatatgata
     3481 agagtatgtc caaactgttc aaatgtggat gtaaataagc taaaaacttt ggttggagaa
     3541 gaaaatgtaa agactggttg tataggtcag tgtagagcat ttaaaaaaga agctgttgga
     3601 ataatagaca atgagcttga aataaaggaa acagaagcag agttttttga agcatgtaaa
     3661 gaataaattt taaataaaat ataataaata gagcatctaa attgtataag cattaaacaa
     3721 tttagatgct ctatttttat atatacattt aattatgagt atacttttct attaaataca
     3781 aataaatata actaatacat tta
//
LOCUS       DAEOSP010000267         3799 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.271, whole genome shotgun sequence.
ACCESSION   DAEOSP010000267 DAEOSP010000000
VERSION     DAEOSP010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.271"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(85..1980)
                     /locus_tag="KRH54_003383"
     CDS             complement(85..1980)
                     /locus_tag="KRH54_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8581012.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            complement(2016..2894)
                     /locus_tag="KRH54_003384"
     CDS             complement(2016..2894)
                     /locus_tag="KRH54_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBG8581013.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVETLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKSAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            complement(3437..3607)
                     /locus_tag="KRH54_003385"
     CDS             complement(3437..3607)
                     /locus_tag="KRH54_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581014.1"
                     /translation="MSALLYGVCNKSISVSIVINMQEFKIGVSMAYGVLVIGICYIAL
                     GIIFKLDRKRFT"
BASE COUNT         1162 a          712 c          435 g         1490 t
ORIGIN      
        1 gtagttaact gattctaaat atccttatta ttattctata tacttgttgt tattccatta
       61 attattttta ctattattta taatttaagc accaagtaat tcaacttcac tattttcact
      121 ccttatagta ggctttgtta cccctataat caatgctgtt attactgtac caatcaatat
      181 acaagctata tatgcaactg gtgcactaca tgcaaatggt attacaaata ttcccccatg
      241 tggtgccatt aaagttatat ctaaactcat acatattgcc ccacctatac aagaaccaat
      301 tatatttgct ggaatcattc ttaatgggtc tgcagctgca aacggtattg cgccttctgt
      361 tatgaaagat gcccccatta tagcagttgg tagagatgac tttctttctt ctttagtaaa
      421 cttatttttg aatattaaca tagctaatga tattgctaat ggaggtacca ttcctcctac
      481 cattaatgct gctgatggtt caaatatatt acttgaaaac atagccaatc caaaagctga
      541 agctgtttta ttaataggtc ctcccatatc tgatgccatc atccctgcta atattgcacc
      601 taataatact ttattagttc cagacatact ttgtaaccaa gattgtaaag cttcatttaa
      661 ccatgatgct ggcgtattaa ctacaaagaa cattatagct ccactcgtaa atgcaatcaa
      721 aagtggacat atcaatacag catttactcc ttttaataca tctggtaaat tttctgttat
      781 cttcattact cctaaagcta tatatcctgt tgcaaaacca gctaccatac cacctataaa
      841 acctgaacct ccaataactg ccataaaacc agctatcata gcaggagcaa atccttgtct
      901 tcctgctatt gatttagcta taaaaccagc aagtatcggt ataaataact taaatgctga
      961 atcgcctcca agttgtccaa agaatccagc tatagggttg tatgtttcat gagttgggtc
     1021 tgatgcaaat ataccaaaca taaaagctat ggctatacca attccaccta tcactacaaa
     1081 tggaatcatt tcagatacac cactcattaa gtgcttataa acaccctttt tctctgaccc
     1141 ttgaagcatt tcttttgaat cttctaaagt ctttccacct ggtttaattt ctttagccat
     1201 tccattttca gcgtttataa ttaaattttt agctaatgaa acattcttag catctactac
     1261 tttcttttgt agcactttct ttcctataaa tctactcagt tgaacttctt tatcacaagc
     1321 tattattacg tattcagctc tttttatatc ttctttagtc aacttatttt ctacacctat
     1381 agctccatga gtttctattt taacttcata tccaagttct tgacctgctt cctctaatgc
     1441 tttttttgcc ataaaagtat gagctatacc tgttggacat cctgttatcc caactataag
     1501 acctttattt tgattattac taaaatctcc tgattctgca gctttattat taaatatact
     1561 taatatttcg tatggagttt ctgcacttag tagatgctta agaaaatttt cttccattag
     1621 atatgtagct agtttagata taatcttaag atgttcatct gaacctgatt ctggaactgc
     1681 tatcataaaa aatgacctaa gtttttgacc atcattatct gtaatctctc tatctgttct
     1741 taaatacatc attacaggtt gatttactac atttgactta gcatgaggta tagctattaa
     1801 attacccata ttagttgatg attctttttc gcgtttatta atatcagcta taaacttttc
     1861 tttattattt atataatttc catggtacaa atgatttgcc atataattaa ttacatcttt
     1921 ttttgtttgt aattgtttat ctagtacaat acaatcgctt cgtagtattt tacctatcat
     1981 aaattctccc ctccttttaa tttgttacct cataattaat ttttattttt ataaaagcac
     2041 tttaacttat ctatagcatt gaatagattc attttaaact ctataacttc tcttgcagat
     2101 tttatacatg gtggatatat agtatctggc tcatataagc ttgggttttg ttctaatagt
     2161 tctctgcact tcttataata tgccgactta acatcactcg aaatatttat tttacaaact
     2221 cctagtttaa ctgcttgtgc tatttcttca tctggatttg atgaaccacc atgaagaact
     2281 aaaggtatat ctactacttc tcttatttct tttaataaat ctagtttaag ctctggttta
     2341 aaaccttttg gatatatacc atgtgctgta ccaattgcaa tagctagtgt ttctactcct
     2401 gttttttcaa caaaatcctt agctaatttt gaatctgtat atattatttc atcagtacca
     2461 ccttcacttg atgttcctgt tgttcctatt gttccaagtt ctccttctac ggacacattt
     2521 aatccttttg ctatttctac tatttcccta gatatagcta cattctcttc atatggtaaa
     2581 tgtgagccat caatcattac tgatgtaaat ccacatctta ttgcccttaa tatttgttct
     2641 ttattttccc catgatctaa gtgtattacc ataggtactt tactcttatt tgcttcttct
     2701 atgcaactag ctacaaagct atctcctaga aaacttagct catttgggtg tattgcaagt
     2761 attactggtg catttttttc gttagcactc tgtactactg ctttaagtat ttgcccactt
     2821 cctatattaa atgctggtac agcaaattga ttttcttgtg ccacttttag catttccttc
     2881 atgtttatta acatattgta cctcccgata ttttaaattt taggttgaat ttgcttcttg
     2941 ttaattttgt tgttgtttat atttaaataa tatcagggta atgaatgtca gaaaataaca
     3001 atcgttttcc ttagtgaaaa ggaactttta ttatacactc taagttcctt ttcactaaat
     3061 aatttttaga agtctgcatc atagttaatt atatttctca tgtacatctt ctctttttaa
     3121 gtgttttatg ctttagcctt cctagatttt ataataaaca attaagatac attaactata
     3181 aatttaaaga tactcatgca atagttaatg ctatattaga gtcaaaaaaa ttaaaaagat
     3241 attagctatt tggccaatac cttttcttgg attccaatag tatatacgat ttatagaaat
     3301 cttactcacg attatctaat gttttacttc cataaacttt acataaaaca ccaaagtgac
     3361 accctccaaa ttcatctcta gctaataaag tgatttggtt ttctaagcac caatttataa
     3421 attagccttt gttatcttat gtaaatcttt ttctatctaa tttaaaaatt attcctaatg
     3481 ctatatagca tataccaatt actaatacac cataagccat agatactcct atcttaaatt
     3541 cttgcatatt aattacaata gaaactgaaa tagatttatt acaaacacca taaagcaagg
     3601 ctgacattgt atattatcca attgtatgaa tgaatactaa tatagctcct tttaattaaa
     3661 tgatgctata taaatcatat ttatcccaat ttttaatcta acctatttat atgtatattc
     3721 ctttttaata aaaaaagata ttgactaaat agtcaatatc ttttagttga tttaaatacc
     3781 cacaatttta atctaattt
//
LOCUS       DAEOSP010000268         3760 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.272, whole genome shotgun sequence.
ACCESSION   DAEOSP010000268 DAEOSP010000000
VERSION     DAEOSP010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.272"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..875
                     /locus_tag="KRH54_003386"
     CDS             <1..875
                     /locus_tag="KRH54_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8581015.1"
                     /translation="INIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNNNYEELTS
                     KESVYYVSDSYKKIFFKDFEIEKINLHIKDANEYDINLFKVQKSILEPEINSQATFDL
                     IVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYPFNFTSSI
                     ESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLIKLGANNK
                     DIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIYFIGYFIY
                     YFLTLKYALNMIKE"
     gene            1100..2713
                     /gene="ggt"
                     /locus_tag="KRH54_003387"
     CDS             1100..2713
                     /gene="ggt"
                     /locus_tag="KRH54_003387"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBG8581016.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2846..3697)
                     /locus_tag="KRH54_003388"
     CDS             complement(2846..3697)
                     /locus_tag="KRH54_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBG8581017.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
BASE COUNT         1453 a          467 c          596 g         1244 t
ORIGIN      
        1 atataaatat tgaaatgtat tataaaaatg gaaatagtta tatagaagat agaacgtctg
       61 tagatattat tagagaaagt gattttaaaa aattaagaca atatcagaat aacaattatg
      121 aagaattaac ttcaaaggaa tctgtgtact atgtttctga ttcatacaaa aaaatatttt
      181 ttaaagactt tgagattgaa aaaataaact tgcatattaa agatgcaaat gagtatgata
      241 taaatctgtt taaagtacaa aaaagcatct tggagccaga aataaattca caagctacgt
      301 ttgatttaat tgtagtaaaa gatgacgtat ttaatcagtt aaaattacat ggaaattcta
      361 gattaataag attaattgat ataaaaaatg aaaaaaaaga actggatttg actttaaaat
      421 taaaaaatat agtaagtgaa aatatgaaat ttacttatcc ttttaatttt acatcaagta
      481 ttgaaagtta taataacttg ataaaattaa gtggtctaat gctgtttata ggcatatttt
      541 tgtctgtagt atttctttta tgtacaggta gtataatctt atttaaacaa ttatcaaata
      601 tatatgatga taaggaaaga tatattatgt taataaaact tggagcaaat aataaagata
      661 ttgagaaaat aatatcaaaa cagttaaaag ttatattttt aatgccatta gttgtaggta
      721 cagttcataa tttatttgct atgtctatag cacaaaagtt tataccaagg tcattacttg
      781 taccaattat cataactcta gtcatttatt ttataggata ttttatatat tattttttaa
      841 ctttaaaata tgctctaaac atgataaaag aataaattat ttttaagtta aaagaattag
      901 atatatgttt tgaataaaat aacttattta agtgcagata gaaaatacta gaattgagtt
      961 taagatattt atttaaaaat aaaattaaag ttaggaaaaa gtctatattt tatatcattt
     1021 tgtatcagtt acattacttg atgttatata ataaatctaa gcagaatatc aaataaaaaa
     1081 tgacgaggag ctgaaattta tgaagtttaa tgcctaccaa cataaatact catcaataag
     1141 aaatgtcgtg tatgctaaaa atggagcagt tgcaacatca acaccattag catctcaagc
     1201 tggacttgaa atactaaaaa agggtggaaa tgctgttgat gcagcagttg caacagccgc
     1261 cacattagca gtagtagaac ctacaagcaa tgggattgga ggagatgcat atgcccttgt
     1321 ttggatagag gaagaaaaac gattgtatgg tttaaattct agtggctttg ctccagaaaa
     1381 tatggaatta aaaaattata ataatatgaa agaaatgcca aaatatgggt ttggagcagt
     1441 aactgtacct ggaataccag cagcttggtc agaattaaat aaaaaatatg gtaaacttag
     1501 cttaatggaa tgcttatctc cagcgataaa ctatgcaaga gagggatacg tagtatctcc
     1561 taatgtagct aaggtatgga aaaaatcata tgaactctat gaaaaagaac ttattggaga
     1621 agagttcaaa ccatggtttg atacattttc taaagatggt aaagccccag aggctggaga
     1681 tatatttata tgtgaagaac aggcaagtac attagaagag atagcaaata ctcaggctga
     1741 aagcttttat agaggaagac ttgctgataa aatagatgaa tattctaaaa aatttaatgg
     1801 ggctataaga aagagtgact tagagtgttt ttatccaaca tgggtagaac ctataagtac
     1861 agaatataag ggatataaaa tatttgaaat cccaccaaat ggtcatggaa taacagtttt
     1921 aatggcactt aacatattaa aagaattgga attagaagga aatatagaaa atgtagaaga
     1981 catacataaa attatagaga gtttaaaatt agcttttgca gattctaaaa catatgtaac
     2041 tgatatagag catatgaagg taaagattca ggaattacta agccaagagt atgccaaaaa
     2101 aagaagttta ttaattgaca ataaagaggc tttgtatcca actgcaggag agccatattg
     2161 tggtggtaca gtataccttt gtactgcaga taaggatgga aatatggttt catatataca
     2221 gagcaattac ataaattttg gttcaggaat tgtgatacca agaactggga ttgctttaca
     2281 tagtagagga aataatttta atttagaccc taaacaccac aatgtagtaa aaccatttaa
     2341 aaaaccttat catactataa tacctggatt tttaggtaaa gaagataaag caataggtcc
     2401 atttggtgta atgggagctt ttatgcaacc acaaggacat attcaggtgt taacaaatat
     2461 gattgatttt ggattaaatc cacaagaggc gctagatgct ccaagatggc agtggataaa
     2521 aggaaaagaa atagaagttg aaccagagat gccaaagcat ataatagatt cattaattga
     2581 aaagggtcat gaaataaaag taattcatga tactgtcgac atgggacgag gtcaaataat
     2641 atttaaaaca gaacaggaaa gttatatttg tgggacggaa tcaagatgtg atggtcatat
     2701 tgcagtttat taatattatc aactaaaata tatataataa atttaaagag tatatgaaat
     2761 aataagtaaa ggcgaaagtc tacaatgact gacgccttta caaaatttaa gctatcatat
     2821 gctgatttta tatagattat gagccttatt cttgaccagt tttgatttca ttgtcatcat
     2881 aactaatcca gtcgctaaag ctaccaatat aaatcttgtg aggtatatcc agttctctaa
     2941 gagcaagact gttaacacaa gcagatatcc ctgaaccaca agataaaatt acttcatcat
     3001 aattatttaa atctttaaag aattttttta aaaattctat atctttaagt gagccatttt
     3061 caaagtcact ttttattaaa tccttacaaa aataattttt tgcacttggt atatgaccag
     3121 cttttgagta agcaggttca actaaaccct ggtatctctc attagcacga caatctatta
     3181 ttacagtatc ttttttgtat aatttagact taacatattc cattggaaca actaaatcat
     3241 tgtttatatt tggtctaatt tccttacctg tacaacttgg tatattcact ttctcttcta
     3301 attctccacc ttcctttacg aaagatgtta ttcccccatc tagtacatag acatttttaa
     3361 gccccaaatg ttttaattgg aaaaataatc tgcaagctcc atttaaatct ccatcatcat
     3421 aagttacaat cgtagtgtcg ttatcaacac ccattttttc taatttttct tttagtatta
     3481 aagggtcttg taaaggattt ttaccaccat gttcttttgt aggagatgat aaatccttgt
     3541 ctatatctaa gataaaactg cctttgatgt gtcctttttt ataagaatca ataccataag
     3601 ttctattgat aagatcaaat ctacaatcta ttacaactag attatcattt ttttctagtt
     3661 ttgatataag ttcttttgct gaaataatgt ttttcaaaga atgtacctcc tgtgtttata
     3721 ttatctttat tttaatttat attaattatt aactttataa
//
LOCUS       DAEOSP010000269         3695 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.273, whole genome shotgun sequence.
ACCESSION   DAEOSP010000269 DAEOSP010000000
VERSION     DAEOSP010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.273"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            373..750
                     /locus_tag="KRH54_003389"
     CDS             373..750
                     /locus_tag="KRH54_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8581018.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            743..1891
                     /locus_tag="KRH54_003390"
     CDS             743..1891
                     /locus_tag="KRH54_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HBG8581019.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            1908..2510
                     /locus_tag="KRH54_003391"
     CDS             1908..2510
                     /locus_tag="KRH54_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBG8581020.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(2603..3625)
                     /locus_tag="KRH54_003392"
     CDS             complement(2603..3625)
                     /locus_tag="KRH54_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBG8581021.1"
                     /translation="MDYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
BASE COUNT         1445 a          487 c          527 g         1236 t
ORIGIN      
        1 gatatatttg taaataatca gtataaaata gatgttttaa tatcataaag gcataattag
       61 aaggttatta aaatttatta gatattactc tagtttattt taaaaaaaag tattataata
      121 ttccttgtgt gaaaatatag gaatttttat taaatagttt taatgtgtaa ataacattct
      181 acactattgt aattaaatat gtaaaaagta aaatatttaa atagatatat tttaaaatct
      241 aacttttaat attgtaaaaa aattagaaat ttaacaaaaa gtacaaaaat tcacattttt
      301 ttcataaagt gtttgactaa aaaattcaca tgttgtagaa ttaaggtagc aggagttttg
      361 agggaggtgt aaataatgat aagaaaaata ccacaagcag aactaaaagt aatgaaattc
      421 atttggaaag tagatgttac agtgacatca aaagatgtta ttgaagctat ggaacaaaaa
      481 tatggctgga aacaaaccac cacactgaca cttttatcta ggctggtaaa aagaggattc
      541 ttagatgctc aaaaaataga cagatataca cattacacaa taagagtaaa acacaaagat
      601 tatttaagtt ttgagacaaa ggatttccta agtaatatac atgatgactc tttaagaagc
      661 ttaatttcag cattacatga tgatgaaaat ataggtaaag ataagctaga ttttctagaa
      721 agttattttg ataatttaaa ggatgaataa ttatatgcta tatgtttttg aaaatcttat
      781 aaagacaact attgtatgta gtcttggtat ttgtctatta ttatttttga aaagatattt
      841 atttaagaag tttagtaaga gattcaatta ttatatttgg ttgataatag tctttagaat
      901 gttacttttt ctatttaatt atacaatagt gtatgaagta aaagaaccca aagaaaatgc
      961 attaggaaat aatattacac aaattgatat ttctacagat aataatctta tgctgtatgt
     1021 agcttattta tggttatttg taactatagt cattgcagtt tatacattta taaagtatac
     1081 aagatttaaa aatctagttg ttgatgtatc ttatgatatt gaagataatg atataaactg
     1141 tatctacaaa aacttgttaa aagaattaaa tataaaaaag aaaattgaat taagaggttc
     1201 tgatgaattg ataagtccag caggtatggg attatttaaa tcttacatat tcttaccaga
     1261 ctatccttat agtaaagatg aactaacttg gattttgaaa catgaattaa tgcattttaa
     1321 aaataaagat attttaataa agtttttagt tttgtctgtt agaataatat attggtttaa
     1381 tccacttgta tatgtaatga gcaataaagt aaatctagat tgtgaattat gttgtgatga
     1441 aagtgttttg gtagaatgtt ctttaaaaga taaaaaagaa tatgcactag cacttataaa
     1501 gtcaattaag tttagtaaaa attataatag taagattttg acaaccgagt ttgataagac
     1561 aaatttggaa aagagacttg agagtattgt taaaaagaaa ggcaaaagtg gaatactaat
     1621 tgctgtttta gtatttatga cttctataac atcttttgtt gaagttgatg ctcaagttag
     1681 aattgatgag ggtaataatg gaatgaataa caatattaat gaatttttat tgaataagac
     1741 gactgataaa attataaata tttattataa agatgcacca aatgaaatta ggaaatttta
     1801 tgaagataaa tgcgaattag aaggtaaaat acctagagac tatgacataa tagaaatgga
     1861 tatgaaagat tataatcaat taataaaata attataagga ggattttatg ttaacaaaat
     1921 taagaaaaat agtagctatt atgtgttgct tattgcttac ttttacaagt gtggcatttg
     1981 ctgacacaag taaagagaaa ggaggaagta aagagactgt tccagagaaa aacctagatt
     2041 cttttactct ttctgggtat atatatacat atgaaaatgc gccacaagaa ataagagatg
     2101 aacataaaca aaattgtgaa gagataaata tagaccctaa gccagatgat gagatatttg
     2161 ttccagaatc agctaatcta atgaatgagg acaatagtaa aggtgtttat gcaagttata
     2221 ctgtttctta taatataggt gctaaaacta taacaattat gagtaatgag tatttgacta
     2281 taagtacaac aaaagttata cgaaagggta atagcggaaa agaagtaaaa gctgcacaaa
     2341 tcatgttaac tttacttggt tataatgtag gtatagatag ttcttttgga agtaagacat
     2401 ataatgcagt tgtaagtttc caaaaaaaat acggtttgtc tgcagatgga ataattggtc
     2461 cagctacttg ggataaattg ggtagactaa ctgaccctac tctttcatag gattaaaatc
     2521 taaaaatata ttaaatagtg tctcaatatg atttttcaat ttaaatgaga cactatttaa
     2581 atataaatgt tataaaacta ttttacaata aattccatat aactctacca tctatatcct
     2641 taacattttg acaaccagtc aaaatcatag tctcacataa ctcattccta accttctcta
     2701 taaaaaactc cacaccatca agaccaccgc caaaagaagc agtaacaaaa ggtcttccca
     2761 tcaaaacagc atcagcaccc aatccaagca tcttaactac atcaacacca gtcctaactc
     2821 caccatcaac aagtattgtt atctttccct ttacagactt agctatatct ggaagtacct
     2881 cacaagtacc aggagtataa tcaagtactc ttccaccatg atttgaaaca ataatggcac
     2941 tagcaccaga ctctacagcc atcaaagcat catctacagt cataattcct tttataataa
     3001 aaggtaatct agtggattca accaattctc taatctcatc aattgtttta ggagaaaaag
     3061 gattttcttg aaattgatta ttaataagtc cacaagcatc aaggtctaca ccaactgcaa
     3121 aagcacctgc ttcttcagac aatctaattt tatctattat ctttgaatta ttccaaggct
     3181 ttataaatac aattccattt cccctattat ccttcaatac atctaaatta tccagtaaaa
     3241 aagtatctac attagtatcg ccaaccatag cataaattcc agaattagaa caacctctaa
     3301 caacaggctc tatgtattct ttttcggaaa ctttaccacc catgttcaat atagtacctg
     3361 atacaggtgc agcgaatata ggagaactca tttttctacc aaataattct atggatgtat
     3421 ctggctttga aacattatgt atgactctca tatttatctt tattttttct agactttttc
     3481 tattttcaat gaaagacgaa ccacttcctt taccacccat accaggaaca tctccagcac
     3541 aagctaatcc attacaaatt ttacatactt tacatgttcc attaaagttc tctctagctg
     3601 attttagtaa gtcattgtaa tccatacaaa atctctcctt tttataatat taaaattgag
     3661 tattactata ttgaaaatat cttaattgta aatat
//
LOCUS       DAEOSP010000270         3677 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.274, whole genome shotgun sequence.
ACCESSION   DAEOSP010000270 DAEOSP010000000
VERSION     DAEOSP010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.274"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..974
                     /gene="ytvI"
                     /locus_tag="KRH54_003393"
     CDS             <1..974
                     /gene="ytvI"
                     /locus_tag="KRH54_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBG8581022.1"
                     /translation="FIYKAFPYIAPFFLGAIIALMINPISQKLENKFRINKGISTLVL
                     SFLAVAIVSTVTTIIVINSMKELMGFLNNISANPEDISNTIMYFLNKINDFMKSFQEI
                     ANFDVEQLVNKFSGEVMQITKNLLTSILGLATSIPYIIIFIITLFIATYFIAKDLDKI
                     ENSFYNMFTVDVRKKVKNVKKEAGLSLVGYIRAYTILMAITFFAIWGSFALFGLKYGL
                     IVGFVGALMDLIPFLGIITIYLPVIVYYFLIKNYFIAISMTVIFFVLSLIREILEPKL
                     VSVNVGLNPLATLAAIFIGIQVKGIIGVIFCLGLVCMHDILKKVDIF"
     gene            1065..2291
                     /gene="pepT"
                     /locus_tag="KRH54_003394"
     CDS             1065..2291
                     /gene="pepT"
                     /locus_tag="KRH54_003394"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBG8581023.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            2517..3569
                     /locus_tag="KRH54_003395"
     CDS             2517..3569
                     /locus_tag="KRH54_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBG8581024.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT         1418 a          388 c          687 g         1184 t
ORIGIN      
        1 tttttattta caaagcgttt ccatacatag ctccattttt tctaggtgct ataatagcac
       61 ttatgattaa tcctatatca caaaagttag aaaataagtt tcgtataaat aaaggtatct
      121 ctactcttgt tctaagtttt cttgcagtgg ctatagtcag tacagttact acaataatag
      181 taataaattc aatgaaagaa ttaatgggat ttttaaataa tatatctgct aatcctgagg
      241 atataagtaa tactattatg tattttttaa ataaaataaa tgactttatg aaatcatttc
      301 aagaaatagc caattttgat gtagaacaat tagtaaacaa attcagtggt gaagtaatgc
      361 aaattacaaa aaatcttctt actagtatac ttggattggc tacttctatt ccatatatta
      421 taatatttat aattaccctg tttattgcta cctactttat agcaaaagac cttgataaga
      481 ttgaaaatag tttttataat atgtttactg ttgatgttag aaagaaggta aaaaacgtaa
      541 agaaagaggc agggctttca cttgttggat atataagggc ctatacaata cttatggcta
      601 taacattttt tgcaatctgg ggaagttttg ctttatttgg attgaaatac ggtctaatag
      661 tagggtttgt aggagcttta atggatttaa ttccattcct aggtataata acaatatatc
      721 ttccagtaat tgtttattat ttccttataa aaaattattt tatagctata agtatgactg
      781 tgatattttt tgttttgtct ttgattagag aaatacttga acctaaacta gtatcagtaa
      841 atgttggact aaatccatta gctaccctag cagcgatttt tattggaatc caagtaaaag
      901 gaataattgg agtaattttt tgtttgggtt tggtatgtat gcatgatata cttaagaagg
      961 tggatatttt ttaattataa taattgatag atttttttaa aaataaaatt ttattcacag
     1021 gtaaataaaa aaatattaat cttattaaga aagagggtat atacgtgaaa caaaaagtag
     1081 tggaaagatt tttaaaatat gtatcatttg atacaacatc taattctcag tgtgagaact
     1141 gtccatcttc agaaggtcaa agagttttag ctaagtatat agttgaagag ctaaaaacaa
     1201 tgggtgttga tgatgtgagt ttagatgaaa atagttatat aatggctact ttaaaaggaa
     1261 atactgatgg tgtggatact ataggattca tatcacattt agatacaatt gaagatgtta
     1321 gtggaaaaga tattaaacca agaataatag aaaattatga tggaaaagac atagttttaa
     1381 atgaagcgtt aaatgttatt acgtatgtaa aggattctcc agagttggaa gaatttaaag
     1441 gtgacgattt aatagtcact gatggaacaa ctctacttgg gtctgatgat aaagctggta
     1501 tagctgagat agtgactgca atagagtatc taataaatca tccagaaatt aagcatggag
     1561 atataaaaat aggatttaca ccagatgaag aaattggtag aggtgctgat ttatttgatg
     1621 ttgaaaaatt tggagcaaaa tatgcatata ctttagatgg aggaatagtt ggagaacttg
     1681 aatgtgaaaa ttttaatgct gcaaatgcaa ccattactat acatggtaga aatgttcatc
     1741 caggttcagc taaaaataaa atggtaaatg ctatacatat agctgctgaa atatcagaaa
     1801 tgttcccagc agatgaaaga ccagaaacaa cagaaggata tgaaggattt tggcatttaa
     1861 attctatagg tggaaatgtc gagaatgtaa gtatggctta tattattaga gaccattgta
     1921 aagaaaaatt tgaaaataga aaatctatta tgatagagaa tatagaaaaa ataaataaga
     1981 aatacgataa tagagtggaa ttggatttaa aagactctta ttacaatatg aaagaaaaga
     2041 ttgagccagt tatgtttata gttgatatag ctaaagaagc tatggaggaa ttaggtataa
     2101 aaccacgttt agttcctgta agaggtggta ctgatggagc aagattatct tttaatggat
     2161 taccttgtcc aaatatattt actggtggat taaacttcca tgggaaaaat gagtgtatac
     2221 cagttagttc tatggagaaa gctactaaac taattgtaag aatagctgaa aaatatgctg
     2281 aaagagttta gttaaaaagt atttaaaaat taagcattta aagttataat agctatatca
     2341 ttctttataa gtcaagctga ttataagctt gtgttgtact taaaatggta tagctatttt
     2401 tgtaattatt tttataaaag ataattattt ttataaaaga taattatttt tagtttaggg
     2461 tatcaataaa aagtatgaat atcaataggt attaacattt aaaaggggga attgtattga
     2521 aaattaaatc taaaaaaata atagcatttg cactttcact tgggattatg ttgccaagtt
     2581 gtgtatatgc agacactatt agtaaaaatc aaaatgcaca ggtagatatg aacaattatc
     2641 ttcaaggtag aaaatctcca aaggaggaaa aacttattgg tgaagataga tttgatacag
     2701 cgataaaaat atctcaaagt ggttggaata atggttcaga aagggtgttt ctagttaact
     2761 caaattcatt gccagatgca ttggcttcaa ctcctttggc aagtaaattg gatgcgccaa
     2821 tactgcttac aaataaaaat agtataccat ataatgtaac agaggaaata aaaaggctta
     2881 atccagcaga agttatttta attggaagtg aaggagctat ttcatcaagt gtaaaagaat
     2941 ctcttgaaga tatggaagta ttagtcagta gaatcggtgg agcagataga gaagaaacat
     3001 ctttacttct tactagacag ctagatgata ctggtgattt aggtgtctca aaagtggcag
     3061 tggttaatgg gtataatgga ctggcagatg caacttcaat atcaagccca gctgcaagtg
     3121 ataatacagc tataatatat acaggtaagg atagcataag aagtgaggca aagaatttta
     3181 ttacacaaaa ctcaactaag acatacatag taggtgggga gtacagtata agtaaaaaac
     3241 ttgaaggaca gttagtaaac tcagaaagac tagcagggac agatagaaaa gatactaatg
     3301 cgaaagtttt agaaaagttt tatggaaaat caagtaaggt taataatatg tattttgcaa
     3361 aagatggaag tggtagagaa gctgatttag ttgatgggct tgctgctgga gtattagcat
     3421 caaaaacaaa gtcacctgta gtacttgcaa gtggaagttt aagtagtgca cagaaaagtt
     3481 ttataaaaaa agtaaaggct gataaatttg tacaagttgg tggaggtaaa aatagtaaac
     3541 catatgcaga agcattagcc ttacaataat attgtgtaaa tcaaaattaa tatataataa
     3601 tagcaagtaa accaagttgc acttcaatat atataataaa ataagtaaac taagttatat
     3661 ttcagtataa tatataa
//
LOCUS       DAEOSP010000271         3627 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.275, whole genome shotgun sequence.
ACCESSION   DAEOSP010000271 DAEOSP010000000
VERSION     DAEOSP010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.275"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            113..2020
                     /locus_tag="KRH54_003396"
     CDS             113..2020
                     /locus_tag="KRH54_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG8581025.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVSTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMENDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            2010..2459
                     /locus_tag="KRH54_003397"
     CDS             2010..2459
                     /locus_tag="KRH54_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBG8581026.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNANTKKEILDIFYEV"
     gene            2559..3446
                     /locus_tag="KRH54_003398"
     CDS             2559..3446
                     /locus_tag="KRH54_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBG8581027.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
BASE COUNT         1575 a          323 c          575 g         1154 t
ORIGIN      
        1 attcttttat gatacagaag tatcttagta attttacata gttagtaaat atgttaaaat
       61 agtacttaat ataaacatag aaatatataa acataggtag ggagggctaa gtatgcttgt
      121 gtcgaggcaa attgaatttc ttaaattgat actaaacgaa aataaatata gacctataaa
      181 atactttaaa gataaattaa atgtatctga taaaacttta caaaaagatt tgaagatgat
      241 tgagaaatat ttagaaactt ttaatataaa aattgatata aaaagaggct atggaatatt
      301 aatagaagat ttagcaaaga agaatataga cttaattaat agtttaaata tacaatcgaa
      361 ggaaaataaa tctttatcag tagagcagag aagaatggag attttaaagt atttattact
      421 aaattcaaat aatataactt caataaatca gttagcagat aaatattatg taagtaaaac
      481 ttctatagta aatgatttta aatatataga ggcatggata aaagaataca atttaaaact
      541 taataaaact ttagaaggaa ctaagattat aggtaaagaa gtggatataa ggaaatcgat
      601 tgccaaaatg atggatgatt tattagatga aaattatgaa caacatgata tatctgagtt
      661 aacaagatta gattctgcta catttagtgc attgataaat ttatttgata ttgattctat
      721 tatatttgta gaaactataa ttacagaatt agaatacaat ttaggatata ctataaatca
      781 gccctactat ataaatttaa ttacccatat acttatatgt ttaaagagaa tcgaagaagg
      841 taatcaaata gaatctcagg aagaaagaga aatttctgta gataatttgg acgagttagt
      901 atataaaaat gtcagtacac ttatagataa aatagaaaat agatacagag taaaaataac
      961 gaaagatgaa attatgtaca tatatatttt tttggtatca tctggattta gtagcgaaaa
     1021 taatagtagt tacaatgatg aatcagatag attaagtgaa agtgaaaaag tttcatctat
     1081 aatgatagag aatatgtcac gatttttaaa tataaatctc aaaaatgatg acttactaca
     1141 gaaaagtcta gcatctcatg taaggcctat gttaaacaga ttaagatatg atatccaaat
     1201 aaaaaacccg ttgttaggag agatacaaga gcgattttca gatgtgctgg gattatgtat
     1261 gatgactata aatattatga cagagtatga taatctgaag aatattagta tagatgaagt
     1321 ttcgtatctt gccacatatt ttcaagcggc aatagaaaga aatatgagta gtaaaagagt
     1381 aatagtagtt tgtcatacag gatatggaac atctcagctc cttgcagcca gattaaaaag
     1441 agaatttcct gaatggacta tagttgatat agttcctatg catcagttga gtaagcgaaa
     1501 tttagatgat gtagactttg taatatctac tgtaaactta gatttgaaag ataaattaca
     1561 tatagttgta tcagtactat taatggaaaa tgatattaat aatataaaaa atgctttact
     1621 ttctaaacct aaaaaaagta tgaacttaga tacaaactta ttaggaatgt atttagaaga
     1681 taatatatat tttaattttg atgtagctca gatggaaagt ttaataggtg tgaatttagg
     1741 gagtaaatat gaatctattt cattaggaaa agatttaaat atatatatac ataagcaaag
     1801 taaattaaat aaagggataa tgaatatcaa taatgttact agaacgattg atatttattt
     1861 agaaattagt aatcaaagtt ttttaaagaa tattttaatg gaaatatcaa atttatatag
     1921 gcaaaagaac tatataaact atataataga ttgcaagagc aaagaggata ttaaaatatt
     1981 atttaagaat aatctattgg agaaaacaaa tggaaattag agaaatatta acagaagaaa
     2041 gcattatttt agatttgaaa tcaactacaa aagaaggcat aatacaggaa ttagtagata
     2101 aattatacga tacaggaagt ataactttaa aaagtaagtt tataaaggca ttaaaacata
     2161 gagaaaaaat aggctctact agcataggtt atcaaatggc catgccacat ggaaaaacaa
     2221 aatatgtaaa gaaggcatcc atagtaatag gtaaatctaa aaaaggtgta gattggccaa
     2281 gtatagatgg cttacctgta aaggtaataa ttatgtttgc tatgccacaa tctggaggca
     2341 atgaagatat gatattaata tctaaaatag cttacatttt aggtggtaaa aatataggtg
     2401 aaagactttt aaatgcaaat acaaaaaaag aaatattaga tatattttat gaagtgtagg
     2461 tattgtataa attataaatt tgaggttttt aaaataaaat gtgtattctt ttaaatagta
     2521 taaattaatt tttagacact agagaggaga ggatatatat ggcaatatat ttagaaatgc
     2581 ctaaattaat acatgaattt ccttttcgta gtctatcaaa tgaaggagaa gtattaacaa
     2641 caccacactg gcataaggaa atagaaatat tacttataac agaaggtgta gtaaatctat
     2701 ttataaatga caagcctatg caattaaaaa aaggcgagat agtgataata aaaggaggag
     2761 atattcatta tatactgcca tcaccgggaa gtgaaagatt agtatttcaa tttgatatat
     2821 ctttttttaa tgatttaatt ttattaaatg aggaaaaaga aagcttaatt aatttattca
     2881 gtagtataaa aaaatgtagt agagattggt cagcaaaaat aagaaagtca gttttcgata
     2941 tattgataga tatctacaat gaagacctat acaaaataga aggatatagt tatgcaataa
     3001 aaggtaaaat gtataattta attcctatat tatatagaca gattcctaga gaaattgaag
     3061 aaaatacagt agaatatgaa ataaactcta aagagatatt agagagattg aacaatatat
     3121 tcaaatatgt agaaaaaaat tataaacaac ctataaaatt agaagatgtg tcttatgaag
     3181 taggatttag tgtatattat tttactaaat ttttcaaaaa aaatacagga aaaacattta
     3241 taactttttt aaatgaatat agattagaaa aagcaaagtg gatattactt aataacaata
     3301 tatccattgt agatttggtt gaagaagttg gttttggaag tacaaaaact ttttacagag
     3361 tatttaaaga aaaaattgga gtctctccat tagaatttaa aaaaagatat aatagcttgg
     3421 aaaaaaaagg gagtgtctca aaatgaactt agttcatgag gcactttctt ttatatgaaa
     3481 tcaaatatat tttcagtatt tcaacaatga aaattccatt catttataaa gtctaaattt
     3541 tagttctcaa tacaacctct atttcgaaat aaatcctttt atatataaat atacagcaat
     3601 atatgagaat aagataacaa taatgag
//
LOCUS       DAEOSP010000272         3489 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.276, whole genome shotgun sequence.
ACCESSION   DAEOSP010000272 DAEOSP010000000
VERSION     DAEOSP010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.276"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..955
                     /locus_tag="KRH54_003399"
     CDS             <1..955
                     /locus_tag="KRH54_003399"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581028.1"
                     /translation="DEINIFWYSYRDVIKLYLGNITQKDNAYIFTGATYLDVDDLEHY
                     PFVMMGDIHIVDDSLCKYSKTVLVLSNQEFAKVLKDQIISCIDDNIKILSNYSFAMLI
                     LPMNLLYGNDMKIIHEYSNNIFLNFFSRKDLNLDTYFEEFNSIEDIALALNPGIESYI
                     VFDEGEDLTKNLEERFKSFIDKGKVPLPAHKSDAFKFWLIIYGFITQALDIILSCKGF
                     NLTPYLRYRVTLQYVLLLGDNVFDIEDLSNMLLKCKVAHVLYAIFDKSEIKNISFVQF
                     YKKLSEYKFSYKVENDIKENININQIAEILNKNITLFLNYI"
     gene            1095..2267
                     /locus_tag="KRH54_003400"
     CDS             1095..2267
                     /locus_tag="KRH54_003400"
                     /inference="COORDINATES: protein motif:HMM:NF013752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG8581029.1"
                     /translation="MLKQNTIQIDYTTYGNNYQFKLPLNIDYMIPKDAIFVDGTKIES
                     SANRYTFVWKKTINKNMVRVMNDISNFILKCGIDFGIKIIYRNKIKIYHLKKLLKKLK
                     KISKESNITFVHGKGKKKSIVQKSIAKLNNYIDRLKKYTKDLHIMGELNSYSKTDNDA
                     TFMRMKKDHMKNGQLKPAYNIQFGVDSEYIVWVSSEHKPTDTTTSIPFLKSIEEHTKF
                     KYPKIVADSGYESEENYTFLECNNQLSFIKPANYEISKTRKYKNDIGRIDNMDYDEKK
                     DYYICKNGKKLFANKIINRKSKTGYKSKITCYTCKECCDCQYKNGCIKGNNSKIPLEN
                     RTKNLQVSKLFHEKRKENLKRIISQEGCQLRMNRIIQAEGTFAQVKQNMKFRRFLS"
     gene            2709..3020
                     /locus_tag="KRH54_003401"
     CDS             2709..3020
                     /locus_tag="KRH54_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBG8581030.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIARPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            3068..3385
                     /locus_tag="KRH54_003402"
     CDS             3068..3385
                     /locus_tag="KRH54_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBG8581031.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
BASE COUNT         1501 a          347 c          518 g         1123 t
ORIGIN      
        1 agatgaaata aatatatttt ggtattcata tagagatgtt ataaagcttt acttagggaa
       61 tattacacaa aaagacaatg catatatttt tacaggtgca acatatctag atgttgatga
      121 tttagaacat tatccatttg ttatgatggg agatatacat atagtagatg attcgctatg
      181 taaatattca aaaacagtac tagtattatc aaatcaggaa tttgctaaag tattaaaaga
      241 ccaaattatt tcttgtatag atgataatat caaaatattg tctaattatt cttttgcgat
      301 gttaatatta ccaatgaatt tactatatgg taatgacatg aaaataatac atgaatattc
      361 aaataatata tttctgaact ttttttcaag aaaagactta aacttagata cttactttga
      421 agaatttaac tcaatcgaag atatagctct agcattgaat ccaggtatag agagttatat
      481 tgtttttgac gaaggtgaag atttaactaa aaatctggaa gaaagattta aaagctttat
      541 tgataagggg aaggtacccc taccagcaca taaaagtgat gcttttaaat tttggctgat
      601 aatttatgga tttataactc aggcattaga tattatattg tcatgtaaag gatttaacct
      661 aactccttat ttaagatata gagttacatt acaatatgtt ttattactag gcgataatgt
      721 ttttgatatt gaagatctca gcaatatgtt gttaaaatgt aaagtagctc atgtacttta
      781 tgcaatattt gataaaagcg aaataaaaaa tatttccttt gttcaatttt ataagaaatt
      841 aagtgaatat aaatttagct ataaagtaga aaatgatatc aaagaaaata taaatataaa
      901 tcaaattgct gaaattttaa ataaaaatat tacattgttt ttaaattata tataaattta
      961 tttatataat tacattcctt agggtacata acagaaaaaa ggctgtcgca ctaaaaaata
     1021 gtgctacgcc cttttttata caaaaaaata aagctcaagt tcaaaagaac ctgggattcc
     1081 tgtataataa ttgtatgcta aaacaaaata ctatacaaat tgattatact acttatggta
     1141 ataattatca atttaaatta ccactaaata ttgattatat gattccaaaa gatgctatat
     1201 ttgttgatgg tacaaaaata gagtcatctg ccaatagata tacttttgtt tggaaaaaaa
     1261 ctataaacaa aaatatggtt agagtgatga atgatatatc taatttcata ttaaaatgtg
     1321 ggattgattt tggtattaaa ataatttata gaaataaaat caaaatatat catttaaaaa
     1381 aacttttaaa gaagcttaaa aaaatatcaa aagaatcaaa tataacattt gttcatggaa
     1441 aaggaaagaa aaaatctatt gttcaaaaat ctatagcgaa attaaataat tatatcgata
     1501 gattgaaaaa atatactaaa gaccttcata taatgggtga actaaatagt tattctaaaa
     1561 cagataatga cgcaactttc atgagaatga agaaagacca tatgaaaaat gggcaattga
     1621 aaccagcata taatatacag tttggcgtag attcagaata catagtttgg gtaagttcag
     1681 agcataaacc tactgatact acaacatcaa taccattttt aaaatctatt gaagagcata
     1741 caaaatttaa atatcctaaa atagtagctg attctgggta tgaaagtgag gaaaactata
     1801 catttttaga gtgtaataat caattatctt ttataaagcc tgcaaattat gaaattagta
     1861 aaactagaaa atataaaaat gatattgggc gtattgataa tatggattat gatgaaaaaa
     1921 aagattatta tatttgtaaa aatggcaaaa aactatttgc taataaaata atcaatagaa
     1981 aatcaaaaac aggatataaa agtaaaataa cttgttatac ttgtaaagaa tgctgtgatt
     2041 gtcaatataa aaacggttgt attaagggaa ataatagtaa gattcctctt gaaaatagaa
     2101 ctaaaaattt acaagtatct aagttatttc atgaaaaaag aaaagaaaat ttaaagagaa
     2161 ttataagcca agaaggttgt caacttagaa tgaatagaat tatacaagca gaaggaactt
     2221 tcgctcaagt aaaacaaaat atgaaattta gaagattttt aagctgaggc aaagaaaatg
     2281 ttatatcaga atgtattatt gtagcgatag ctcataacat ttctagacta cacaacaaaa
     2341 taataaaaaa taaatgtgga atatatcttc atcatttaaa agaagtattc taaatttatt
     2401 atttatagtt agatattatc tgaaaaaatg gtatttttat acctttattt gttatgcata
     2461 attttactta gaacataagc tttatataaa acaattaaaa atatatctaa ttaagataag
     2521 taaaaaaagc tatcacagaa caaatttaat ttgctaatgc gacagcccat ttttgttaat
     2581 ttgacactta ataatattaa aataacaaaa aagtattgaa agtatattgc atttaaggta
     2641 gaattaaata aaatattata acattataat gtttatatag ttgttaataa aaatttggag
     2701 gaaaatatat gataaaaata atgttggcct gttcagcagg gatgtctaca agtttattag
     2761 ttactaaaat ggaaagtgca gctaaagaaa atggtataga gtcgcaaatt tgggcaatac
     2821 cagaatcaac tatacaaaat gaaatagaaa aatgtgatgt attattacta ggaccacaag
     2881 ttagatatgt attacctaag gctcaagaaa ttgctaggcc atataatata ccagtagaag
     2941 ttataaatat gatgcactat ggaacagtta atggagaagc tgttttaaat agagcgatag
     3001 aacttaacaa cactaagtaa tataaattat aatgtatttt taatatataa attgggagaa
     3061 tcaaaggatg gatgaaaaga ttatagaaat atcttttaat ataataggtt atgcaggtga
     3121 aggtaaagga ttagcttttg aagcaataaa agaggctaag aatggaaata tagaaaaagc
     3181 aaaagaatta ttaaaagagt ctagggaagt tataaataaa gcacatagat atcaaacaga
     3241 attaatacaa aacgaagcta gtggaaacag gactgaaata agtgtcatac ttatacatgc
     3301 acaggaccac ttaatgaatg gcatgaattt tcaacagtta gctgaggaaa tcgttgattt
     3361 acatttgaaa ttacaagata aataaaaatt aaagataata aattaagaga gttcttaaaa
     3421 gatacattat gtgtagcaca ctaattgtta gattattgat tcaatattta gtgagtaata
     3481 cattaggat
//
LOCUS       DAEOSP010000273         3467 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.277, whole genome shotgun sequence.
ACCESSION   DAEOSP010000273 DAEOSP010000000
VERSION     DAEOSP010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.277"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            73..408
                     /locus_tag="KRH54_003403"
     CDS             73..408
                     /locus_tag="KRH54_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8581032.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            545..1822
                     /locus_tag="KRH54_003404"
     CDS             545..1822
                     /locus_tag="KRH54_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBG8581033.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKEQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            1943..3331
                     /locus_tag="KRH54_003405"
     CDS             1943..3331
                     /locus_tag="KRH54_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBG8581034.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRKTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
BASE COUNT         1431 a          332 c          600 g         1104 t
ORIGIN      
        1 tttataatat taataaatat attgctataa aagtttgttt aataaattaa ataaagaaaa
       61 gaggagaata aaatgtacaa catagatgat atgatatata attgtccagt agaagcttta
      121 tcaaatattt taggtaaaaa atgggtggct ataattatat ggaaaataca agaagataaa
      181 aaacgatttg gagagctaca aagagctata ccagactgta gtaagaaaat gttagttcaa
      241 caactagatt ttttaattga acaggggatt atagtcaacc aaaaaaagtt ggttaataat
      301 gttgtagagt caacatattt tttaagtgaa tcaggattac gattgcttcc tgtcatggaa
      361 aaaatgatta tgtggagcaa taaaaattta aattgtgata aatactaaaa tttactctgt
      421 aagtaatgaa aatttaatta agctatgagt agaaaaatgt attaaaatta cataattttt
      481 tagttagatt ttaatttaga aatgggattg taatattgga aataaaataa ggaggaattt
      541 taatttgcaa aatgtaaatt tatttttagt atttatagaa ggtatagtat cattcttttc
      601 accatgtata ctgccaattt tgcctattta tttgagtata ttgtcaaata gtagtgtaga
      661 aaatttaaaa gaaggaaaga cgagttttat aggaagttct ttatttaaaa atactatatt
      721 ttttgctctt ggaatctcaa caacattctt tatattgggt tcatcagtaa aagtattgag
      781 tatgtttttt aatgaaaata aagatttaat aatgtttatt ggtgggatta taattattat
      841 catgggatta ttttacatgg gaataattaa atcttcaata ttgaatagag aaaaaagatt
      901 taatgttaag tttaaagaaa tgaaagcaat aacagctttt atattaggtt ttacgtttag
      961 ttttggatgg acaccttgta ttggtccaat attggcatct gtattagtca tggtttcaag
     1021 ttcaagtaat catcttagtg ccaatctttt aatagcagta tatactattg gttttatttt
     1081 gccattcata attacagcta tgttttacag taaactattt aaaactatag ataagataaa
     1141 atctaatatg gaaattatta aaaaaatagg tgggattata cttatagtat caggaatatt
     1201 aatgatggta aatggatttg gaagtataag caaacacttt aatacgtcac aaaatagtaa
     1261 aattgaaagc aagcaagaag aaaataagcg agaaaattct actgataaag aagaaaattc
     1321 tgatggtaat gatagtcaaa aagactctaa taatgacaat aatgataaag ggtctaatga
     1381 tgaagataga ataaagtcta ttgattttac tttaacagac cagtatggta aaacacataa
     1441 attaagtgat tatgaaggca aagttgtatt cttaaatttc tgggctacat ggtgtcctcc
     1501 atgtaaagaa gaaatgcctt atatagaaca actgtataaa gactataata aaaataatga
     1561 tgatgtagta attttaggag tagcttcacc taatttaggt agagaaggtt caagggaaca
     1621 tgtagttaac ttcttaaaag aacaaggtta tacattccct gttgtacttg atgaagatgg
     1681 agcattagca tatcaatatg gaattaatgc ttttccaact acatttataa ttgacaagga
     1741 aggatatgta actcagtata ttccaggagc tatggataag gcaactatgg catcatttat
     1801 tgaaaatcag agaaataaat aaaagtattt aagtataagt ttataaatca atttaaggtt
     1861 acaaaaaagt gcctaattgt accacctaaa taatcttgtt atagtaaaag tataaacaat
     1921 acaagaaagg gttgaatttt atatgaaaaa aacatttgat gctataataa taggatttgg
     1981 aaaaggtgga aaaacattag caggggattt ggcaaacagg ggattaaaag tagctttaat
     2041 agaaaagtca aacaagatgt atggtggaac atgtgtaaat gtggcatgta taccaacaaa
     2101 aagcttagaa aattcagcaa acagcgtaaa aacaaagaat ataaattctt gggatgaagt
     2161 acaagctgaa tatgagaaag ctatagataa aaaagaaact ttaattacaa aactcagaga
     2221 agctaactat aataaattaa attcaaatga gaatgtaact atattcacag gaatgggaac
     2281 tttcattgat gaaaaaacag ttcaagtcaa aactgaaaat gaaatttatg agttagtagc
     2341 agataatata tttattaata ctggttcaag accatttata cctaatatta agggaattga
     2401 aaataagaat atagtatatg atagtgagtc tcttatgaat ctaagagttt tacctaaaaa
     2461 aatgacaatt ataggagctg gatttatagg tttagaattt gctggtattt atagttcatt
     2521 tggagcagaa gttacaatat taaactctaa taatggcatt ttaccaaatg aagatgttga
     2581 ggattcagaa gaaataataa aattacttgc aaagagaaat gtaaaaattg ttaataatgc
     2641 aaatataaaa gagattaaag aagtttctga attagcaata gtagaatatg aagtagatgg
     2701 taaatctaaa gaattaacaa gtaatatgat acttgtagct acaggaagaa aagcaaacac
     2761 tgaagggcta gggctagaaa atgcaggtat agaattaaat gaaagaggat ttattaaagt
     2821 ctcagaaact cttaagacta ataaagagca tatatgggct ataggagata taaatggagg
     2881 tccacaattt acttatattt cattggatga ttatcgcata gttattaatc agttatttgg
     2941 agataagact agaaaaacta atgatagaaa gaatatccca aattctatat ttataagtcc
     3001 agcattttca agagttggtc ttaatgttaa gcaagctaaa gaaaagggat atgaagtatt
     3061 agtggcaaag atgccagtag aagcaattcc aagagctaaa cagattggca aggcagatgg
     3121 gtttataaaa atagtgatag ataaaaaatc caataagata cttggagcta gtatgatatg
     3181 tgaaaattca agtgagatta tccatcttat tcaattagct gtagatttag aagtagagta
     3241 tacatattta agagaccgtg tttatgcaca tccaactatg acagaagctc ttaatgatat
     3301 tctatctcct aatatgataa aagaagtata acataagttt aatatataaa cctaaaatta
     3361 tcattataag tagattttag aaagagacta tctcaaaaaa tagagataag tctctttttt
     3421 actgtttaaa taataaaaat atatttgatt ttataaaaga agattat
//
LOCUS       DAEOSP010000274         3410 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.278, whole genome shotgun sequence.
ACCESSION   DAEOSP010000274 DAEOSP010000000
VERSION     DAEOSP010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.278"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(277..846)
                     /locus_tag="KRH54_003406"
     CDS             complement(277..846)
                     /locus_tag="KRH54_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBG8581035.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            1249..1821
                     /locus_tag="KRH54_003407"
     CDS             1249..1821
                     /locus_tag="KRH54_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HBG8581036.1"
                     /translation="MEYIINPCSGIKIKITRGQKIKIVDIEGGQVVDFFAEMKDDSTE
                     FISPGVTIDCNESLRLQVGNLIYSNKYRPMFKILYDDVREHDLLHPCCRPEMYDFFYH
                     NGENHPNCFDNINRTLGTSHQIIHPINFFMYTKVNTDGSIEVCEPLSKASDKIILEAL
                     ENMTLGIAACSVSESSCNSRKCTSIKILVD"
     gene            complement(2500..3402)
                     /locus_tag="KRH54_003408"
     CDS             complement(2500..3402)
                     /locus_tag="KRH54_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBG8581037.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHKYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTAHIF"
BASE COUNT         1224 a          620 c          385 g         1181 t
ORIGIN      
        1 taataactta taattaaata tttacacata tattcattaa cttaaaattt aatcaaatgt
       61 gatattttat ttaccttagt aaaaatgtgt gtgtctcaaa ataaactttt tagttgatga
      121 ggcactcttt tttacatgaa atcaaatata tttttatcct ttcaacaatg aaaaagggat
      181 ttatctctcc attttgaaac aagccctttt tctattaatt actttcttaa aaaatataat
      241 acgatatata agataaatat ctaattgtac ctatttctaa ttatatctat tagaatacaa
      301 cacctttgcc ttttcagtaa actccctaac aaaatctagt ttactatctg tataagcatc
      361 acgattatgc tcatacttct tccaaaggtc caacttaagt ttttcatact cctttgcaac
      421 atctgggtgg tcaattaagt aatctctaaa aaacaactca tcattatcac ctacatacct
      481 tatatgcaaa tgaaaaactc gttcttcaaa cccatctact gtataccctt tattaagtga
      541 aagcctacta ctgctctcag acatacaaat atatccattc gactcaatca aatctttaat
      601 atctttcata ttataatcag gaattatctc aatcagtatg tcaacaatgg gcttggcata
      661 aatcgtactc acagaagtac ttccaatatg acttattcta acaatttttt gcattggcaa
      721 tattccagtt agtctatatt gttcttcttt aaaccaattt ttccaacatt ctttgtgagt
      781 agtcaaaaat ataggaaaca agttccataa ctcctctaaa gtcatatctt ccaacttctt
      841 tttcattagt tacctccctt taaatttgta ctttatattt tttctttttc aaatttactt
      901 agttaatatt taactttatt ttttatcaaa tcatttttct aattatgtaa atttttctaa
      961 atttagtttt taatgtatta taacagattt ttacccttct tacacttttg tatctattta
     1021 aatattagaa cattttttct tacttctaaa tacagtaaga ttgcttaaat accaaatagg
     1081 agcaaaaaaa ctctgtaatc ttaaaaaaac tacaacatct ttatgagaat taaaaaatga
     1141 tagactatgt tatcaacatt aagaaacaga tagaaaagaa acagagtatg tgctaaaatg
     1201 ggaacataga acagaatgac aaattggaat ttatggagga cattaaatat ggagtatata
     1261 atcaatcctt gtagtggtat aaaaattaaa ataactagag ggcagaaaat taaaattgtg
     1321 gatattgaag gaggacaggt cgttgatttt tttgcagaaa tgaaagatga ttcaactgaa
     1381 tttatatctc caggtgttac aattgattgt aatgagtcat taagattaca agttgggaat
     1441 ttaatatatt ctaataagta tagacctatg tttaaaattt tatatgatga tgtaagggaa
     1501 catgacttgc tacatccatg ttgtagacct gagatgtacg atttctttta tcacaatgga
     1561 gaaaatcatc caaactgttt tgataatatt aatcgcacac ttggaacttc ccatcaaatt
     1621 attcacccca ttaatttttt tatgtacaca aaagtcaata cagatgggag tattgaagta
     1681 tgtgaacctt tatcaaaagc cagtgacaaa attattcttg aagcattgga aaacatgact
     1741 ttagggattg ctgcatgcag tgtttcagaa agttcttgta atagcagaaa atgtacttct
     1801 attaagattt tggttgattg acaaatttca tttatcgaaa aaatatcaaa attaaaaaat
     1861 gacgtcaaag gcgcaaaagg gtaaccactt gcgctctcaa ttcattatgg tttgttacgt
     1921 atttatgggt aaaaatccct tgattctatg atgtttttca gacacaatta caaatgagta
     1981 aggattttat actcatactc taaaaaatag cattttattg cgtaacagaa cgaaaaaaca
     2041 gtgtgtacct ttttcttgca atacacactg tattcatagc cactctagct gttaactgtt
     2101 aattatctca taataccctt actggtattc atctaatctt aacccctttt aattattttc
     2161 atttataaat aaattctatt cttcccaaaa tatcgtaatt aaaatatatt aattaaaata
     2221 tcccaataag aatatctgaa aaatcttcta cctatttttt caaacctttt attcccattc
     2281 ctcatacaat agtgcaactt ttagcacagt ataaatatac cattagtcca tataatctca
     2341 ttaacaaata tatacaatta ttttcttcaa actcttttat atctaagata tcacattttc
     2401 attttcttat attaattcct taactctctt tcaaattacc aattttttaa tcaaatcttc
     2461 attaaatatt ctatcattat taaacattca tcattatccc taaaaaatat gtgccgtatg
     2521 ttctttacac aaattttcca tcttatcata cacagcacca ctatctctct catctgtcat
     2581 agtcacaatt acatcacttg gtctaagaat tgtcttacct ctaggaataa tttcttctcc
     2641 acctctttga attgctacca aaagacaatt ttcaggccac tgtacatcct gaattgcctt
     2701 gttcgcaaca ggagagccaa acataacaga gaactggtct agtatctttt cgcccctctc
     2761 tttatgtaca ggttctccat tcttttcaag taacctctca agaagacttt cataaatagg
     2821 tttagacttc ataagtgtag ccacaatata tgccacaatt gaaattactg atagtgaaag
     2881 catttgacta acagaacctg tcatttcaaa aattaatatg ataccagtca aaggggcacg
     2941 cacaatagct gtaaaatatc ctgccatagc caaaagcaca aagttattta cataatcggg
     3001 attcattcca aaaactttaa cacctaccat accaaatatt ccacctataa atgctcctaa
     3061 aacaagcaat ggaaaaaata ttccccctgg tgcacctgac ccaaaactaa cagccgaaaa
     3121 tataaaacgt ataacaaaaa tcaaaacaac cattccaaaa atatatttat gacttgtaag
     3181 ttcttcaact aaattgtgtc cactccccag aacatcaggc ataacaagcc caagaatacc
     3241 agccattata aatggaatca acagttttcc aaacgtacca attttaggaa tcttcttgta
     3301 taaagactgc acatatagag taaaccagtt gtaaaatgcc cccataactc caagaataac
     3361 acctaaaatc actattaatc cataataatc ttgtggcaac acatttccaa
//
LOCUS       DAEOSP010000275         3316 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.279, whole genome shotgun sequence.
ACCESSION   DAEOSP010000275 DAEOSP010000000
VERSION     DAEOSP010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.279"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     repeat_region   1..422
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1..26
                     /rpt_unit_seq="tacattccatatagttaatataaaac"
     gene            complement(1081..3174)
                     /locus_tag="KRH54_003409"
     CDS             complement(1081..3174)
                     /locus_tag="KRH54_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBG8581038.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKVLQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         1161 a          528 c          326 g         1301 t
ORIGIN      
        1 tacattccat atagttaata taaaactaga tatttaaaat aagcgaaagg gggtcataaa
       61 aatatttaca ttccatatag ttaatataaa acattctaaa gatattttgc cttgcagttg
      121 ggatttcgaa tttacattcc atatagttaa tataaaacca tcataggggc taatatagtt
      181 aaaggtgttg gcgtatttac attccatata gttaatataa aacggtttgg ttacaaggta
      241 tagtagaagg atatagtgga atttacattc catatagtta atataaaaca tggaactaag
      301 agagtacacc atcacatgat actcaaattt acattccata tagttaatat aaaactaaag
      361 ctggaaaaga aacagctata atgagtaata ataatttaca ttccatatag ttaatataaa
      421 accccaaaat aaacttagta ttttcaatac ttacacgtat acaactctct taaatttgca
      481 gtgaacgagc aatagtgcaa ttgataacgc ttatcataca ctctcaacgc cttatatttc
      541 aactgttacg ccaaattttt tcacaaatat cgctcactgc aaaattccta taattttatt
      601 atatcataaa tatattactt ttgaatattg atactaattt ataccataat aaaagcaaaa
      661 aaacgtaatc tatctacatt agatttaaat tctataactt aaagtttttt acataatatt
      721 tatgcttgtt taatcaatct aatatatcat tcttgtatat tgcttttaat agtctttttt
      781 cttaaataaa tatctatctg aacaagcata gtttaattaa aaacttttgt attcccttaa
      841 aactgtatct acctcttatt attttattta tgatatatta tttttaatct aaaaatagat
      901 atctattttt attatagtta tccattttta aaatacttta atcatatact actcccaatt
      961 taacatccat tgaaatgcta ttttcttata ttacaattta taattgatat atcttcaata
     1021 taattattta atgtattttt gattttaaag cttttcagtg atattattga taaattagaa
     1081 ttaattatct agctttaaaa ggtcaaagat ttctgttgct gtctgcggtt ttccaaataa
     1141 atatccttgt atgtaatctg gctttatttg tttaactaaa tcatactctt cttgtgtttc
     1201 tacaccttct aaacatactt ttattcctac gtcatgacaa attgcaacaa taaaatgtat
     1261 aaatgtagca tcaaacttac tctttaatat atctttaaca aatactctat caattttcac
     1321 tatatctatt ggagaaaatt ttaaaacttc tagagacgag tatcccgtac caaaatcatc
     1381 catagcaata taaattccta aatcttgtaa aaccttaaat ttaaactgta aaatactcat
     1441 attttgtact gtatgacttt ctgtcaattc taaaacaaga ttttgataag gaaaattttc
     1501 atcatctata atttttacta tatcttcaat aaatgtgtct tctaatattt gaacagctga
     1561 aacgtttaca ctcactttaa aaaaaggata gtaatcaatc catttcctaa aagtacgtaa
     1621 tgccattcga agcacccaag cccctacaat atttatcata tcattttctt ctaatattgg
     1681 aataaattcc aaaggagaaa ttgctttgca tttgtcatta gtccacctta aaagaacttc
     1741 tacacctatt attttatgac tttctgtatc tacttgtggc tggaatcgaa gactaaaccc
     1801 cctaaaatca tcattaattg atgcctttaa atcccgcatc atttctaatg aatacatttt
     1861 attttttaat atttcttggg agaaaaaaac aagcctgttt ttaccatgct ctttcgcata
     1921 ttgtaaagag taactagcac acttataaag ttcttttact gtatctccat gctttggata
     1981 tattacacaa ccagcagaaa ttgttatatt taatccatat tttctccata gttgaagatg
     2041 tataatcata ttctgtattt gattatatag agttagaatt tcattttctt caacattatc
     2101 aacaagaata cccatttcgt ccccatcaag tttataaagt tcagcattat ctggtaaaat
     2161 agattgtata gactgtgcta gtgtttttat tatattatct ccaaaatccc tatcatacat
     2221 ctcattaaca tttttaaaat cgtcaatatt taataatact atgcataatt tttcaatcat
     2281 aggatttgaa attttatctt caaatacact cataaattca tgataattca gtaactgtgt
     2341 caatgaatca atcttatttt gtttacctaa ttgcgtcatt attcctgcaa aaatacttgg
     2401 ttctccaaat tcatcacgca ttagttgacc tctacatctt aaccatatat attctccact
     2461 acgattttta gccctaaatt ccactgtatg ataatccatc tggtttttac ctatttcagt
     2521 gtttgactta taaaaacgat tccagtcttc tggatgtaca atttttttcc agtaaacaag
     2581 cggattttca acaatttctc ctggtaagtc aaataattca acttgtgatg gtgaatatcg
     2641 aaatacgcct gttttcatat tgcaaatgta tatatattca tctgtacctc taatcaaagc
     2701 atcataaagc aatgtagaat tataatcaaa attgtctctt gaaaaattaa aaactccttt
     2761 ttcttcttta ttatttgata tttcatgaaa ttcattaatc ttaattggtt tatttaagct
     2821 tgttgagttt gcaatatcta ttgattgatg catatgaaca actgtgccat cttgccatgt
     2881 aattaaacta tcataatttt caaaaacacg atttaactta ttacattttt cataccactt
     2941 tattaatact ccttttttat cattctttaa taattctaaa actttacaaa aactacaagt
     3001 agaatttttt tcaggatata atacttgcca acaaactttt ccttctggat ctaaaatatt
     3061 atattcttct ttcatttttt tgttcataaa aataatttca ttagtatgaa tatttgtaac
     3121 atatatatta atctgtagtt gatttaatat aacttcaaaa ttatgtttgt tcatatactt
     3181 atctcctcca tagttactgc tctttataac aacattgtta aaattattct ttttagaaga
     3241 tttacttata acttatacat atacactaaa taccttaatt taattagaaa gtttattact
     3301 tcatatttta aattat
//
LOCUS       DAEOSP010000276         3263 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.280, whole genome shotgun sequence.
ACCESSION   DAEOSP010000276 DAEOSP010000000
VERSION     DAEOSP010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.280"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            134..781
                     /locus_tag="KRH54_003410"
     CDS             134..781
                     /locus_tag="KRH54_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBG8581039.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(948..1517)
                     /locus_tag="KRH54_003411"
     CDS             complement(948..1517)
                     /locus_tag="KRH54_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBG8581040.1"
                     /translation="MKKFIICIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(1522..2421)
                     /locus_tag="KRH54_003412"
     CDS             complement(1522..2421)
                     /locus_tag="KRH54_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBG8581041.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(2432..2581)
                     /locus_tag="KRH54_003413"
     CDS             complement(2432..2581)
                     /locus_tag="KRH54_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581042.1"
                     /translation="MSNFIKDNKKSISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(2574..3158)
                     /locus_tag="KRH54_003414"
     CDS             complement(2574..3158)
                     /locus_tag="KRH54_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBG8581043.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
BASE COUNT         1140 a          557 c          368 g         1198 t
ORIGIN      
        1 ctatattgta actattttcg taatcttagg aaaaggttac ttataaaatt aaccttatac
       61 aataatatat cgtataatct taattagcat aatagttaca atattaaaat ttaaatatgg
      121 gagtgatttt agtatgataa gacaaacacc attagagaaa cgatatgaca agctaggtcc
      181 tcatattgta acagcactca aaaaacgtta ttttgatgct tattactgta aaactagaaa
      241 tgaagctttg caacaaattt tagatttaat tcctcaaaat catgtagtat catggggagg
      301 atctgaaaca cttaaagaaa tgggcgtaca aactgctgtt agagagaaag gatttaaagt
      361 tattgataga gatttagcaa aaactccaga agaaagaact gaaattatgc gtcaagcact
      421 tctatgcgat acttttttga tgagtagcaa tgcaattagt gaagatggac aactctttaa
      481 tattgatgga aatggcaatc gtgtagctgc aatgatattt ggtccaaaaa atgttatagt
      541 tgtagctggt atgaataaaa ttgtaaaaaa cattgatgat gcaatacaaa gaactagaac
      601 aattgcatct ccagcagttg ttcagcgttt tcaagatgtc aatacaccat gctatattaa
      661 tggaagttgt atagactgca caagtcctga aagcatttgt gcatacatgg ttactacaag
      721 aatcagtcgt ccagcaaaaa aaataaaggt aatattggta ggagaaaatc ttggtctata
      781 aaatatacat acatgtaaat agctctataa ataataaaat catatgaaat aacttatttc
      841 taaaagtggt agaataaaag cttctacaag actttcattc taccactttt atattctttt
      901 aatttgtgtt tctatattat taattgctcc gattttcata tatattacta actattttta
      961 ttttcataat ttttttgttc ttcatcaagc tgtttataat aatctttatt atagtcagct
     1021 agataatcta taccacctcc tgtaatcaca tttaataatt tcattgcaga ttcttgatta
     1081 actttacaaa cataattatc accagattta acaagctcta atgtaatatt aaattctttt
     1141 ttctttgcag aatcaatatc tttcatagtt ggatttggca tcatttctcc tagtttagga
     1201 ggattctttt ttaaaataga aaccatatca gtattgttaa tttttaaatc tacagtcact
     1261 ttatccttat tattctttat atctgaaatt tcccaagtca tatttttaga atacatttta
     1321 aatccttctt tatcttcata tataagatta cctaaccctg attttgggaa atacttatca
     1381 acagtatctg catcttgtgt ttttattgaa ttaaacatac tatccacttc attttttatc
     1441 ttattatcat tagtacaacc agctaacact agtactgata ttgcaaacat tataatacat
     1501 ataataaatt tcttcattaa attatatccc cctatacttt tttgcacaaa tattattctc
     1561 ttttttatct tttgaaaaag ttgtagtaat tgcattttgt ggacaaactt ttatacattc
     1621 accacaccta atacagtctg gactatttac atctttatat attggtatat caagcttaca
     1681 ttttgtttga catgctgtac aatttgtaca tttatcttta tcaattttaa atctatatat
     1741 tgaaatagga ttaaaaaaac catagattgc tcctaaaggg catatatact tgcaaaatgg
     1801 cctataagta atgattgaca tcaataatat caatagcaaa atacttaact tccagctaaa
     1861 taaaaatcct attgattctc ttaaattctc atttcctaaa ataagtggta ttcctcccaa
     1921 aagtgttcct gaagggcaaa tccatttaca aaaccaagga tttccacccc ctgctacatt
     1981 tacaatagca actggcaaaa taattacaaa cattattaag actatatact ttagatattt
     2041 tagatattta tcaccataaa ctttttttat ctttactgga aagggtactt tatgtagtaa
     2101 atcttgtaca agtccaaagg gacaaagcca accacaaaca aatctgccaa aaaaagcacc
     2161 aaatgccatt aaaaaaccaa caacataaaa tgagaattta tactctctgc ttgataaaac
     2221 tgcttgtaat gaacctattg ggcatgctcc aagtgcacct ggacatgaat aacaattcaa
     2281 tccaggtaca cataattttt tactttctcc tccatatata gttccttcta aaaatccatt
     2341 tacatatcca tttgttaatg ctgtaaatgc aacttgagta ataagtctta tattattatt
     2401 tactttagat aataacttca ttcctcttct cctaaccaat accaatacac tcaagacaaa
     2461 tcataattgc tttagttaat actgtatttg tttctccacg atatgtacct aatgcaataa
     2521 atataatact aattgcaata atagaaatgc tttttttatt atctttaata aaattactca
     2581 ttggctttta ccatttttag tctttcatct ataatttttt tccaatcttc cttactattt
     2641 gcacctacaa ccttctcacc cacaaagttt cctttcttgt ctacaaaata agttgttgga
     2701 aactccataa ctcttttaaa atcagttttt ttaagttctt cagatacagt caattgttgg
     2761 taagttgcat ctgacttttt cataatgtct ttaactaaat ttttctcttc atttgaaaga
     2821 ccagttctca tatcagtttt atcaacttct acaactaatc cctttatagc tacattggaa
     2881 ttattactct catattcttt tgataatgct tcaagctctg gcatttcacc cacacatggt
     2941 ccacaccaag tagtccagac attaataaga gtaagttctt tatttgaaaa tatctctttt
     3001 gttaccttat ttccatcaac atcctccgta tctaaattac taaaccattc actattattc
     3061 ttgttttttt ctactggctt agattctttt ttgctacatc cagaaatcat acctaaaaca
     3121 caaatacatc ctaaaactac tatgaaaaac cttttcatat ctcacactcc tagaatttat
     3181 atagtatata aaatcgctgc ttgaaataaa aataacttat cttctatatt aaagtaatta
     3241 tatttaataa ataagagcta att
//
LOCUS       DAEOSP010000277         3260 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.281, whole genome shotgun sequence.
ACCESSION   DAEOSP010000277 DAEOSP010000000
VERSION     DAEOSP010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.281"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..1247)
                     /locus_tag="KRH54_003415"
     CDS             complement(<1..1247)
                     /locus_tag="KRH54_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBG8581044.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSETFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCG"
     repeat_region   1894..3109
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1894..1922
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
BASE COUNT         1152 a          394 c          427 g         1287 t
ORIGIN      
        1 tttccacata catatctatg aatgatattt ctatcatcac caacatagat aaatctttca
       61 agtctttcaa gtatagaata attgtcatga tttaaatttg aatcatcaat aatcaatgca
      121 tcaaaatcat aaccttcttc aaagctacca acttttccaa agaaacttcc accacttttg
      181 gttgcaatat aaaaagtctc agataaagat aagaaattat acttcttacc agagttaacc
      241 caatataatt tagataattg tattgcagat accattgctt taaatattga caatgtatgt
      301 ccaccactta tatctgatcc aagtgctaat ctaatattat tatcgattaa ttttctgata
      361 ggcatagcac cactaccaac attaaagtta gaagctggac agtgaactgc cataacatta
      421 ttttctctca ttaaatctat ttcatcctga gaacaatgaa cacaatgagc cattaaagta
      481 ggagtttgac caaataaatt atatctatta tatgcatctc catagaattt agattcaggt
      541 tctagacttc taacccattc aatctcatca taattttcag ataagtgtga ttgaatagga
      601 atattatatt ttaaacataa atctcctaaa ccttttaaaa gttcacttgt acagcttggt
      661 ataaatctag gtgttattat tggttttaca atagaatctg tatcactata cttatttata
      721 atttcttctg tatcagtaat agaatcctga gtgttttcta ataaagtatc agggcaattc
      781 atattcatat ttactttacc aacgtatgct ccaagaccaa cttctttgaa tatatccata
      841 agtttcatag aggcttcttt atgtactgtt gcaaacactg ctattcttgt agttccagat
      901 ttccaaagtt cttttacaaa ttttttatac atttttgttg catattctat atctttataa
      961 tttgattctt ctggaaatgt ataagtttca agccatggca aaagctcttt atccatagcc
     1021 attccaagat ttttaaattg agaggcatgg gcatggaggt cattcatacc aggtattata
     1081 agcttatcag tgtaatctac tacttttagg tttttgtatt ccttaggtat ctctttaaat
     1141 attcccttta ctttaccttt ttccagtata atataactat tttcaaatac tgtgaaagtt
     1201 tctgaagttt ttgtaaaaat tatatttcct tttaatactt ttaacatata ggtctcctta
     1261 gttttaaatt tgttttaata tatgtaggta aaattttgtt ttacaaatca aaattttacc
     1321 acatgtatta tatcaaatct aaactataaa tactattaga taatagcaaa tattagtgaa
     1381 atacagttgt ttactattat tatatataac tgacatttaa gtgacattta agaaaaatat
     1441 aatgcctact tacataaaat ggaatgttat ttaaagagaa ctttgattat attttcagaa
     1501 gcttttttat ccatatcgtt taaaatatga gaatatctat ttatagttat ttttatatta
     1561 gtatgtccta atctttcaga gatgattttt atattagttc tagctagaag aagaactatt
     1621 agaatagata atatagtaag tatttacaaa tatgtaggtg ttcttaaatt gataaattat
     1681 tccattttaa ttttatagtt tgaattttat gatataataa aaatatataa attttgcagt
     1741 gagcgatatt tttgataaag tagggtttaa cagttgcaat gtaagggatt gagggtgtat
     1801 gataaatgtt atcaattgca ctactcatgg ttcactgcaa atttgagaga gttgtatgtg
     1861 tgtaagtact gaaaatactt agtttatttt ggggttttat attaactaag tggtatgtaa
     1921 atagaattta gtactttcta aaaatggatt gcctagcaag ttttatatta actaagtggt
     1981 atgtaaattt tcctaattcc ctatggtgta gcattggttg atagagtttt atattaacta
     2041 agtggtatgt aaatatatta aagatatgac actcttttat ataaaatata agttttatat
     2101 taactaagtg gtatgtaaat acaagttcag tagggagaca gacagtaatt ttaaaagagt
     2161 tttatattaa ctaagtggta tgtaaatcac aacaatacaa ataaaactct gaaaaggtaa
     2221 aagttttata ttaactaagt ggtatgtaaa tttattatta ttatatatct tgacttagca
     2281 ctatttttgt tttatattaa ctaagtggta tgtaaatatt actttgaaag cattaacaga
     2341 aaaagagatt agtaagtttt atattaacta agtggtatgt aaattattta ctgttgaacc
     2401 tacatcttga ttggataaaa agttttatat taactaagtg gtatgtaaat taaggtttta
     2461 atttattagt aataaatata agacctagtt ttatattaac taagtggtat gtaaataatt
     2521 ctttgactgc atcatttaaa taatctagct gagttttata ttaactaagt ggtatgtaaa
     2581 ttctaaaagg tcacatatat ttattaaatc tgttgccggt tttatattaa ctaagtggta
     2641 tgtaaatcat ataatgaatt acataacatc ttatgatatt catcgtttta tattaactaa
     2701 gtggtatgta aatttcatag tctttagagt ctgtaaatac tttactcata gttttatatt
     2761 aactaagtgg tatgtaaatt tactttaaaa tatgtaccat aaaaaaatat atcaagtttt
     2821 atattaacta agtggtatgt aaatatctct ggcgtataac cttcttttcc tattagttct
     2881 ggttttatat taactaagtg gtatgtaaat agggtttctg ataaaatctt caaacatgta
     2941 aaatatgtgt tttatattaa ctatgaggta tgtaaatctt atacttagtt agaactatat
     3001 atcgacacaa atatgcttta tattaactat atggtatata aattgcaatt tttattcgtt
     3061 gtccaatcgc tttgaaattt gttttatatt aactaagtgg tatgtaaatt tgcaacaagt
     3121 ataggtaaaa taccccaata atttatacag cattttctcc tttaaaatat aattattttt
     3181 tatcatttgt agtaaataat taccacataa tattgacttt agttttaatg attaaaatat
     3241 aaaagtagaa taattataaa
//
LOCUS       DAEOSP010000278         3247 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.282, whole genome shotgun sequence.
ACCESSION   DAEOSP010000278 DAEOSP010000000
VERSION     DAEOSP010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.282"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(406..627)
                     /locus_tag="KRH54_003416"
     CDS             complement(406..627)
                     /locus_tag="KRH54_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBG8581045.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            1207..1464
                     /locus_tag="KRH54_003417"
     CDS             1207..1464
                     /locus_tag="KRH54_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG8581046.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENNV"
     gene            1933..3171
                     /locus_tag="KRH54_003418"
     CDS             1933..3171
                     /locus_tag="KRH54_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBG8581047.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYLSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDKNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISVLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         1322 a          292 c          489 g         1144 t
ORIGIN      
        1 tgttttgctt gttagtatta gtatttcaat tgtcaatatt agtgttttgc ttgttagtat
       61 taatatttca attgtcaata ttagtgtttt gcttgttagt atcagtgttt caattgtcaa
      121 tattagtatt ttaattgttg actctagtta ttgctgatat agaaaatatt taatttattt
      181 caattaatta gattaaagta gggtagcatc taaaatagct aacatgttta agttgaaata
      241 cgtttaatat attaattttt caaagcatta atatattaaa gttataatca aaattgaagt
      301 tataatctgc caaatctata gcgtattaag tgcgttatat aacttaatct tttatataaa
      361 gagttaattt ttttaatttg taaagcagag tacgtttaaa aggtactatt tagataatga
      421 tgtactatct aattttaaaa tatctacaat agggttctct tgaagacttg ctattgcttc
      481 tgcaacagca taaacatctt catttaatgc ctctgctttt acatttgcaa atgatttact
      541 tttaacaact gccttaccct tatcatcaaa accagcatcc aattttagtt ttatactaga
      601 taaatttttt gttgaaacaa cttccataaa attacctcct aaaaatttaa tttcaaagat
      661 ttgtcgactt tctttgttag ctataatact atcatctaag ataatatcgg agttaaacag
      721 taatttctaa agattttttg tgtaaatttt tataggaaaa attttacttt tttgctgatg
      781 aaaaagttta caagttattt gtgaagatat gttcttagtg ttttgaaata gtatttgtat
      841 atattcatgc ttagttgaga tagttttgta tggattttca tatgtgtaga gttaaatgtt
      901 aggtactaaa gtttatgtta aattaaatgt taaagcttat gttaaaaatt ttattaaatt
      961 taatctgtaa ataaaaaagt taaaagtcca atattaacat tttactttta aaccattaaa
     1021 atctttatta aagctaacaa agttataagt tatgagaaat tttattaaag ttaatacaaa
     1081 aattaatttt aaagctaaga aattttttat tagattcaat aaaactaata aatatagttt
     1141 agtcaataat gtattaaata aaaactttgt taagtctata aaaatatctt aaaggaggtg
     1201 taacttatga agataaaagt ggaggatata ccagttcaat ttcatgcaat ggtagaaata
     1261 attggaattg ataagtttat agaggttgcc aagttgtatg gaggaacaaa tacttatata
     1321 cctacgtata aggggctttt tagatatgct agaaataggg atatagtaaa acaatttaat
     1381 ggagtcaacc acaatgaatt ggctattaga tataatatgt gtgtaagcaa tattaagcga
     1441 atactcaatg aaaataatgt ttaaatataa taaaataatg tttaaacata atgaaaatag
     1501 ccttgttaaa cgtaagagta aagacaaaga agatgtttta taaagttttt ttagacaaat
     1561 taactaaatt tactacttat taatttaaat acattgtata ttctataaaa aaatttgtta
     1621 aatactggag atatattgag aatacaaaga aaatggtcga aaatatttgt tggctaaaaa
     1681 atagataaaa tgatacaatt gcttgtataa aggggaaaaa taaacaaata aactatataa
     1741 ataaagaaat agcaattaat atgacataat atatttataa aattaagaat ataaatagat
     1801 attttaaggt attagttgtt aaattaggaa atattaaaaa aaatatatat ttatatttaa
     1861 actattttgt tatttatata aaaatactta tctagttact agcaattatt agtagaaatg
     1921 ggggaaatag agatgaataa gggattttac aaattaaatt cagtggtatt gaactttgat
     1981 attaagaatg atgttaacaa gataagattt aatcattata taatgagaaa agaaaacata
     2041 caaagacttc aaggaactct gccaaacgga caattttata tgacagtgag aagagctgct
     2101 acagatttac aactttctat ttctactata agtaggcttg taaaagagtt tttggattta
     2161 gggataataa gattagtgtc taagggtgta aaaggtaatc gttctgtata tagttacgta
     2221 agtatagagt ttaatggaag tttatttgat gatagtaaat atatggaact tgactgtgta
     2281 tgtaatgatg aatttattga taatagtggc atagagtttg attgtgatag caaagataat
     2341 tcttttaatg gagatataaa tgtagaaact gtgcattgca ttaatgagta tttgtctgat
     2401 gatgatgtag agcataatta taacaatttc cataataaag atactataaa ggataaagcc
     2461 atttctaata ttagcactat caaaaaatat attgatgaat cttttgataa aaataaatca
     2521 gaaaattcta acttacaaca ggagtcaata aatttcaact ttaatgatac atgcaatgaa
     2581 atagataaaa gaacgaataa tagtatagaa aataatcaaa ataaaaggcc tatgagattg
     2641 agtgacatca gtgttttaag tgataaaggt atgacaaata aaaagaatta ctttaatcat
     2701 aatcatggta tacaaaaagg gactaaatct gtaacaaaga aaaaagaatc cttaaataaa
     2761 tttaataaaa aaaatataca gcaaagcatt ataaatatgc taaataaaaa atcaggtaag
     2821 aattttaaaa tcgattctcc aataagcata aaacttatca atgaaaggct aaaggaaggc
     2881 tatgtcttag aagatttcta caaagttatt gaggtaaaag tggctaagtg gaaaaatacc
     2941 ataatggaga tgtatataag accagaaacc ttgtttagct ataagtttga atcctatgta
     3001 aatgaaaaca tatctatgaa aagtcaaagt aatgttcacc catgtaaaga aggcaatagt
     3061 aatgatagca gtaattatat acattctgat tatacaacat atgatggcag tgtgcgtgag
     3121 agaaaaaaat atgagaatgt aagatactgg aatcctatat gtgagattta attagtttaa
     3181 gaatgtattt atgataaata agatattaat agagaatagt gagctatagc aaatgatata
     3241 tcaatag
//
LOCUS       DAEOSP010000279         3221 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.283, whole genome shotgun sequence.
ACCESSION   DAEOSP010000279 DAEOSP010000000
VERSION     DAEOSP010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.283"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(376..1098)
                     /locus_tag="KRH54_003419"
     CDS             complement(376..1098)
                     /locus_tag="KRH54_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBG8581048.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            complement(1157..1834)
                     /locus_tag="KRH54_003420"
     CDS             complement(1157..1834)
                     /locus_tag="KRH54_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBG8581049.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            complement(1862..2677)
                     /locus_tag="KRH54_003421"
     CDS             complement(1862..2677)
                     /locus_tag="KRH54_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBG8581050.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     misc_binding    complement(2903..3113)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1006 a          605 c          330 g         1280 t
ORIGIN      
        1 ttactataaa agttaaattt ataagcttac ttgttataaa aataactttt ataatttatt
       61 tcaaaaaaaa tattgatttt tgtgtcgcat tttgttataa taaattaagg ttatccccct
      121 ggtaacccta gttagttaat atatctattc ccaataccct ttcataagga tatattttat
      181 atttattaaa aatcggatta gtaacggaaa ttaattcggt ggaaaaaaag agcttacccc
      241 gagctctttt ttttctgcaa aaaatcgtaa aaataaagtt aatattaata taactttaat
      301 tttaaaatta atattaacca tcactaaaaa aggatgtatt gaatttctaa tatcaataac
      361 atccttttta tataattata gcacttttgc taaaaatgct tttgttcttt catgttttgg
      421 atttccgaaa acttcttctg gagtattatc ttcaagtata cttcctccat ctataaaaat
      481 aactctatct gcaacttcct tagcaaatcc catctcatga gttacaattg ccattgtcat
      541 accttcttta gcaagttctt ttataacatc caaaacttcg tttaccattt ctgggtcaag
      601 agcagatgtt ggctcatcaa acaacatcat atctggttcc atagcaagtg ctctagctat
      661 tgctattctt tgcttttgtc ctccagataa ttgtgatgga tatgaatctt ttttatctaa
      721 aagaccaact ctactcaaca aaatttctgc ttttttttct gcttcttcct tagtcatctt
      781 ttttaattta attggtgcta atgttatatt atctaaaata gtcttatgtg ggaataaatt
      841 aaagttctga aacaccatac ctatattttg tctaacctca tcaatattag tttttttatc
      901 gaccaaattt tttccttcaa atattatttc tccacctgta ggtatttcta gtaaattcat
      961 acatctaaga agtgtacttt taccagaacc acttggacct actataacca ttatttcgcc
     1021 tttagcgatt tctaagtcta tattttttaa tacatttaaa tctccaaaag atttacttaa
     1081 attttttatt gtaatcatat tatcactttc ctttttacta ttctatgttt tccctaaatg
     1141 ctaaacatgc tctcatctag ttagacacag atagtttctt ttctaataat ccaactaatc
     1201 ttgatagagt aaatgttata acaaagtaaa ttgctgctgc tactattaat ggctctaatg
     1261 ctctaaatgc tgcatttttt actatatcag cagtacgcat tatctcaaca actcctatta
     1321 cagatattat tgaagattct tttacaagtg taataaactc atttgctaat gaaggtaata
     1381 tattttttat agcttgaggt attattatat atctcatagt tgaccaataa tttaatccta
     1441 atgatctact tgcttccatt tgtccattat caactgattg tatacctgat ctaagtatct
     1501 ctgccacgta tgcaccagag tttactgata atgcaaatgc actagctata aattcacttg
     1561 gtataccaaa caacataggg aatcttattc ctaattgagg taaaccaaac catactatat
     1621 atatctgtac taataatggt gtccctctta aaacctcaat atagattgat gatattgctc
     1681 ttaatacttt gctctttgat atttttgcca tacagataat tatacctatt ataaatccaa
     1741 agcataatgc cacaagagaa attgatactg tcacacctgt tccttccaaa aaagaagtcc
     1801 caaatctact taaaaaacta aaatctaaac tcataatttc atctcccaca taactttctt
     1861 actaatttga agcagcttca gtagatgctt catttaaaaa ttcttctatc ttcttgccat
     1921 cttgtaattc ttttatcttt ttgtttaata attctataaa atctttgtta ttgtcagatt
     1981 ttttgattgc tgctgccata ccttctgtta catctttccc aacttcagta tttgccattt
     2041 ttattccatc atatttttta acatttatta aagaaactgc ttcattagta acaactgcat
     2101 ctatgtttcc attttttaat tctgtaatta aatcaggtat tgctttcaaa gattttgtag
     2161 ttgtcatttt taaagtgtta actacatatt cttcttggat acttcctgct tgaactccaa
     2221 ctctaagttt ctttaaatca tcttcagttt ttactttgtc tatatctgca tctttaacta
     2281 taactgcatt tctacttaaa taatataatt ctgaaaaatc aacactcttt tctctctctg
     2341 gtgttggaga catacctgca agaacaatat caacttggtc tgcatttaaa gcacctatta
     2401 atccatcaaa tgacatatcc ttaatttcaa cctttacacc aagctctttt gcaaattctt
     2461 cagccaacat tatatcgaat ccttttattg aatcttttcc atctactacc ttatgaaatt
     2521 caaatggagg aaattctgca ctagtaccaa caactaattt tccattgtct tttatttgtt
     2581 ctaactttgt tttttctcct ccaccactac atcctactag cgataatgtc aatcctaata
     2641 ctaatcctaa acttaataat tttttaaata ctttcatttt aactacccct ctcttttaat
     2701 tataatttcc ctcagatttt aatacttaaa tgtttcgagt tacaaaaaaa gtctctcagt
     2761 ctttaatgac taagagacga ctttaccgcg ttaccactct tattgataaa tacttatatt
     2821 actttcaaaa tttgtgactg ttagcattta agctgtatgg atatttttat ttttaaatca
     2881 aaaaagtccc ttagctttac agctaagaga cgaattatat ccgcgttacc actctttttg
     2941 ataagtaagt acttatcctc tcaatccctt aaggcgggta aaccaattat catactaaag
     3001 tttctgataa tctcctccag agctctcttc acatagactt cattgctagg cttacaccaa
     3061 cccctaactc gctgtaaatc catatatgct actttctcta tcattggatt tgtttcctta
     3121 aattgttaat acaattgtac ttccttataa tatttttgtc aataagtttt ttattttttt
     3181 aataattttt acttgtaaat tatctaaata ctactgttta t
//
LOCUS       DAEOSP010000280         3104 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.284, whole genome shotgun sequence.
ACCESSION   DAEOSP010000280 DAEOSP010000000
VERSION     DAEOSP010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.284"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            62..604
                     /locus_tag="KRH54_003422"
     CDS             62..604
                     /locus_tag="KRH54_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8581051.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            618..>3104
                     /locus_tag="KRH54_003423"
     CDS             618..>3104
                     /locus_tag="KRH54_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8581052.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKT"
BASE COUNT         1261 a          398 c          491 g          954 t
ORIGIN      
        1 taaattttaa aggagtataa taaacagtgt tcataataat tttaataaaa gaggtggtaa
       61 tatgccaaag attttagaaa atgtaaaaga agatatatta aaagtgtctc gggatatgat
      121 tttagaagaa gattactcga atattagcat aaggaaaata gctcaaaggt gtggtataag
      181 tgcaggtaca gtgtataatt actttaattc aaaacaagaa ataatagaat atatcacaaa
      241 atctgaatgg gatttattga taaggcgaat tgaatactcc aataaaaaca ctgaagatta
      301 cataaaaaaa ctaaacatta tttttactga aataagaaat tttataaaca aagttcataa
      361 tatccaatac aatgactttt taaacacttt tgaaacagag agattttttg aaatgaaaaa
      421 acacaaagac gactttcatg aacagctttc aaataaagta tatgaagctt tagaaaaaga
      481 aagttttttt aataatgaca aattagtatg caatatcata ataaaaatgt ttttttcata
      541 ttcaacatat cttcatatag aatttgaaga tttaaaaccc tatattgaaa aattaataca
      601 ttaaggtggt gtttaccttg aatcataata ctagtattaa gaacaaggac agtaatccaa
      661 aaatattttt agtacttacg ctttatctat taggtatatt tatgggagct attgatactg
      721 gaatagtatc accagcaaga acgattatac aaaatagcct tggagtaaat gaaaaaacgg
      781 gaatttggat gattaccata tacacattaa catacgcttc tgtaattcct atctctggaa
      841 aattagcaga taaaatagga agaaaatatg tgtatctagt tagtatattt ctatttggta
      901 gtggttcatt aatatgtgga ttatcatcac tattttccaa tttctatatt ttattaattg
      961 gtcgtgttat tcaagcaatt ggtggtggtg gaatcatgcc aattgctaca gcagaatttg
     1021 gaactacctt cccagaaaat aaaagaggca tggctttagg tttagttggg gcaacatatg
     1081 gtattgccaa tattttaggt tcaagcattg gtagcacaat tctaagtatc tttggaactc
     1141 aaaattggaa gtggctattt tttgtaaatc ttccaatttg tttaatcata cttattggtg
     1201 gagttttctg tattaaaaac aataagtctg aatctaaaga aaaaatagat aagcttggta
     1261 cattgatgtt gatatgtata atcgtatcac tattatatgg tcttatgaat atagactttt
     1321 ttaattttaa aaattctata caagatatat cagtttatcc atacttattg acatttattg
     1381 tacttatacc tattttcata tttattgaaa atagagctaa agaccctatt ttaagctttg
     1441 aatatttttt aaatccgaga atattgatag tacttatttt atctttaatt gttggtattg
     1501 gaatgatggg tatggtattc gttccacagt atgctgaaaa tgcattaaag ataaatgctg
     1561 gttctggggg atattttgta gctatattgg gattatttgc aggtattgct gctcctttaa
     1621 gcggaagact tattgacaag tttggggcaa aaaaaatact attactagga ttttctgtat
     1681 ctatgatagg ctctctttac ctagttttaa ttgctctaaa aaccaatact gtatttagtg
     1741 tatgtgtaag tttaatattt atgggattag gaatgggatt tactatggga actcctctaa
     1801 actatatgat gttatcaaat acaagattag aagaatctaa ttcagcactt gcaacattat
     1861 ccttgatacg ctcaattggt acttcaattt ctccagctat aatgattgga tttatagcac
     1921 atgcaggatt atctgtgcaa gataatatta tggacttagt tggaaagccc tcaactccta
     1981 aaatagttca acttgaagaa cttgaaaaca tgatagatga cttaaaatca gaccctgaaa
     2041 tggcaaaaaa tttaaaaaat gtctctatcc caaatatgaa tgatacttca aatataaata
     2101 tggacatgac aggtggcaaa cttccaaaat ccttacttaa taaagttcag tcagcagata
     2161 caaccaacat taccaatata acaaaagaaa tatcaacaac aatgtttgat gaaaaagttc
     2221 catctataat tactaaaata cagaaaaatg tacaaaaagg aatagatgga acacaaagtg
     2281 gaataaacgg tattgaagat ggtgaaaata aactaaatag tggtattaaa ggagttcaaa
     2341 ctggtattga caatatggta aaagctagag ctggtattaa tcaaggaata gatggtgtaa
     2401 aaaaaggtat tgctggtgtt gataaaggta taaaaggtat ggaacaaggt cttgaaaaac
     2461 aagatgaggc catcaaggaa cttacaactg cttataataa aatacctact acagaaaaaa
     2521 ctcctactga taacgaaaat aactcaaatg aacaaaacca gagtaatacc agtaattctg
     2581 aatcagacga cactaagaat gattctacag aaaataagaa taacaattcc agcgagaata
     2641 aaccaaatgt aaatgaacaa aaagaaagtt taaatgctca aatacagcaa ctaaaaaaga
     2701 caagagatga cttaaataca aagttacaaa aaaataaaac tcaaaagaac gaattaagta
     2761 aaaaactaag atcaatggaa tctcaaaaga aagagctaca aagcaagtta aataattcta
     2821 taagccaaaa gaaagacatg gaaaaaagcc ttgatacaat gcaacagcaa aaaaaatcac
     2881 tccaagcagt tttagcaaag acacaagagg taaaaactga aataccaaag gcttttgata
     2941 aatcaaaatt ggattatata aattccatag aaaataatag aacaaaaata gaaaacacac
     3001 tacaatctac tctaaactca ggatttaaac agatgtatat aacagtattc tgtgtaaatc
     3061 tactagcatt tataatatta ttgttctata aagaaaataa aact
//
LOCUS       DAEOSP010000281         3095 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.285, whole genome shotgun sequence.
ACCESSION   DAEOSP010000281 DAEOSP010000000
VERSION     DAEOSP010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.285"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            32..748
                     /locus_tag="KRH54_003424"
     CDS             32..748
                     /locus_tag="KRH54_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8581053.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            723..3056
                     /locus_tag="KRH54_003425"
     CDS             723..3056
                     /locus_tag="KRH54_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG8581054.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALDIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
BASE COUNT         1288 a          280 c          542 g          985 t
ORIGIN      
        1 aaatttataa attaatataa aggagattag aatgaattca tataatattt tggtggtaga
       61 agatgaaaag gagatagcag atgctataga aatatatcta ttaaatcaag gatataatgt
      121 ttttaaggga tacaatggat tagaaggtct taaggttata gaaaaccaag aaatacattt
      181 agctatagtt gatataatga tgcctaaaat ggatggtata acacttacaa tgaagcttag
      241 agaaaactat aattttccag taataatgct atctgcaaaa tcagaggaag ttgacaaaat
      301 aatggggctt aatataggag cagacgatta tgtaacaaaa ccttttaagc cattagaact
      361 attggctaga gttaattcac aacttagaag gtacactaag tatctaaaca tggttggaaa
      421 taaagagcaa aaggtagaaa gtgacgatag tgtgtttgca ataggcggat tagaattaaa
      481 tgaaaataca aaagaagtta gtattgatgg aaagtatata aaaacaactc ctatagaatt
      541 taaaatacta agtttactta tgagaaatgc aggaagagtt ttttctgctg atgaaatata
      601 tgaaagagtt tggaatgaga atgctgtaaa tacagataca gttatggtac atgtgaggaa
      661 tataagggaa aagatagaaa ttgacccaaa aaatcctaaa tatttaaagg tggtgtgggg
      721 tgttggatac aaaattgaaa aaatttaaag ataaatatat agttactatt atagttttta
      781 tacttatgtt atcagcatca ttaggtatga ttagtcaata ttctactata caagcaagtg
      841 ctaaaggtgg agcagagaat ccatttgaac aggaaagctt tgtagatagt atatataaag
      901 gaagttatgt tttatatcat aatatgaagg aagaacaaga aggtaagatg atacaaccat
      961 caaaactatt tatcaatgaa gaaactatga actatataaa aaatgtaagt aaagaaaaag
     1021 atacttatga tggttcagaa acttactata cagatgatag tattaatgca gattttaatg
     1081 aaaactttga aaattgggag catttcatta aagcgtcagg aaagaattta aagtactatt
     1141 tagttgataa agaaagcaat tttgaatttt ttaatgataa taaagaatta ggagtacttg
     1201 atacaacaaa aaatgatgct tcaaaagaaa attctgatga aaatcagtca aaaaatacag
     1261 aaatgaatga tgaggaaaga caaaagcaga ttacagatat aaaagaaaac tatagattct
     1321 acgttgtaat gaattttgat aaagatggaa aagtaaatat tgtggattct tatggagtaa
     1381 atcaaaaaat tattaatcaa atgttgttat ctaaagcaag agaagattta atggatacaa
     1441 gtttaggagg aaattcaatt tcatataagt tatctccaat aaaaaatata acattagtat
     1501 atgctgtacc taaagtaata agtaaaggtg atagtgtaga gtatggttat ggaaatactt
     1561 attttgatga tggtataatt tcatatataa gcaatgtaga aaataattct tattataaga
     1621 tttctggaat gattttaaaa attatattag ctatagttgt tattgtagct ttagcattcc
     1681 catatagaaa atctaaagag ttgctaggat ttgatacatt atcaagaata ccgttagaga
     1741 tactctttat tgctcttgat attatacagg ggcttgtaga tttatatcct actgttataa
     1801 taagtaatac cttgagtggg aattatgtag agtaccttat aaacaatgac ataagtgcta
     1861 aaatggctag tatattggta agtttaatga atttaactta ttggtttgta atattctcaa
     1921 taatatttgt atttataata cttttaaaac atattttaaa tgttggaata agagagtatt
     1981 ttacaaaaca tactgtgaca ggaatgtttt ttgtatggct aaaaaaagca ggaaaaacag
     2041 ttattaatca aattagaaca ataaatctaa aagaaaagcc aaataaaact atagttacaa
     2101 ttttgggaat aaatttattg attatagtta taatgtgtag tatgtggttc tttggaataa
     2161 tactcgcatt aatatattca gtagtattat ttaaaatact gtcagattac tctaaaaaga
     2221 caatatatga atataatcaa cttttagatg ttacaaagaa aatttcagat ggaaacttag
     2281 atgtaaatat ggaaaaagat ttaggatttt ttaatcctat aaaagacgaa ttaggaaata
     2341 ttcaaactgg atttaaaaaa gctgttgacg aagaggttaa gagtcaaaag atgaaaacag
     2401 agcttatatc aaatgtatca catgacttaa aaacacctct aacatctata atcacttata
     2461 tagatttatt aaaagatgaa agtataacag atgaaaatcg taagttgtat attgatacat
     2521 tagacagaaa gtcacaaaga ttgcaacatc taatagaaga tttgtttgaa gtaagcaagg
     2581 caaatagtgg agatgtttat ttgaatatag taaatgtgga tattgtatct ctaatgaagc
     2641 agacactttt agaattagat gacaaacttg cagattcatc tctaatagta aagaataact
     2701 tttctaatga aaaaataata ttaccattag atagtcagcg tacatttaga gtatttgaaa
     2761 atcttataat aaatataagc aaatatgcaa tgccaaattc aagagtgtat attgatattt
     2821 tagaaacaga taataaagta aatattatgc ttaaaaatat gtctgcatct gagattgatt
     2881 ttagtgtaga tgatataatg gagagatttg taagaggaga taaatcacgt aacacagaag
     2941 gttctggtct tggtttagcg atagcaaaaa gttttataga attacagggt ggtaagatga
     3001 atataagtgt ggatggtgat ttatttaaag ttacaattac ttttaacaaa aattagatta
     3061 gacttcttta ataattataa agtctatagg ataaa
//
LOCUS       DAEOSP010000282         3078 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.286, whole genome shotgun sequence.
ACCESSION   DAEOSP010000282 DAEOSP010000000
VERSION     DAEOSP010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.286"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            88..1530
                     /locus_tag="KRH54_003426"
     CDS             88..1530
                     /locus_tag="KRH54_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG8581055.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            1572..1925
                     /locus_tag="KRH54_003427"
     CDS             1572..1925
                     /locus_tag="KRH54_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581056.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            2116..2937
                     /locus_tag="KRH54_003428"
     CDS             2116..2937
                     /locus_tag="KRH54_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBG8581057.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKEYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
BASE COUNT         1189 a          315 c          547 g         1027 t
ORIGIN      
        1 taaattataa taaatgacta tagcgtagat gtaatactat gttatagtca tttttgaatt
       61 aaacttgata tatcaagggg gaagaatatg agaacaatta gaagagtttt agcattaggt
      121 ttaacattag ctatatttct aataaatgta cctaatgtag atgcattaac atcagataca
      181 attaaaggta acaatatata tgagacggct ggattaatag cagataaaaa gagttatgat
      241 acagcaatta tggtaaatat ggataattct atagcagatg gtctttcagc aagtggtctt
      301 gcaggtgctg ttgatgctcc aattttgctt gcacaaaaaa ataagatacc aaatgaaaca
      361 aaacaaagat taaaaaatgt taaaaagata tacataatag gtaaagaatt atcaataagt
      421 aagtcagtag aaactgaact aaaaaacaca ggggcacagg taactagatt aggtggagat
      481 gacagaataa agactagtta tagtgttgct aaagaggtaa atggtatcaa aaaagttgat
      541 gaagtgatat taactaatgc atataaagga gaagcagata caataagtgc tgcacctgta
      601 tcagtaagag atatagctcc tatagtactt acagatggaa agagtgtgcc tttttcaaca
      661 agcaatgtaa aaacatatgc agtaggtggg agtatatcaa tgagcactag tttagttaat
      721 aaaactaatg ctaaaagact tggtggttca gatagatatg atacaaataa gaaagttata
      781 aaagagtttt atccagatgc atcagaattt tatttgagtg atggatatga tttagtaaat
      841 gcacttacag gttctacaat tgctaaggaa aatccaattg tattggtgtc agaaagtagt
      901 gataagtcta tattagcagg agcagataag attactagat taggttcaat aagtgacagt
      961 gtgtataata agtgtgtttc tgctgcacaa aataatggcg attcgtctac aaaaggtgag
     1021 tcacctatga aaaatgagac aagcatatta ggtcagccaa cagctagttt agaagcgtgc
     1081 ttaaaatggg caaaatctaa aaaagctaat gatttattta tagagttaat accaatatta
     1141 tatgatactg ctgttcagga aggtgttaat cctgttttgg cagtagctca atctgcaaaa
     1201 gaaactggtt tttgtaattt tggtggagta ttagatgcat catttaagaa tccttgtgga
     1261 cttaaaactt ctgtaggtgg ttctgatact gataagaatg cccattcaag atttgatact
     1321 tgggaagaag gaatattagc tcaaattcaa catttatgct tatatgcagg acaagatggt
     1381 tacccacttt caaatccagt agaccctaga catgaaaaat ctttatttgg taaggctaaa
     1441 acagttgaaa gtttatctaa taattgggct ggaggtcaat atggacaaga tttagtaaga
     1501 atgatgggag aaattgaggc aacaaaataa ttgaagatta tcaaatatcc aataaataag
     1561 gattgattat tatgaaaaag agtaatattt ttagtatcat atttcctaca gtcataatgg
     1621 tgctaatgtt tttaactttt gctacagata tatttaatat tccagatatt caatctaaaa
     1681 gtatttttgt tgtgggaatg gttttgattt ttccaatctc ttttttgata caagggataa
     1741 tttgtgtatt aaataagact aattggattt tatctcttat tgtgtcttta gtaacttata
     1801 tagtattaat gtatatattt ttaaatgatt ctgcatatat atatgtttta ctttatacag
     1861 ctttttatat gattggacat ataacagcta aactctatta caaaattaag acttttaaaa
     1921 attaaattga agtatataaa agtaacatta agagaagtat ccttaatgat tttctaaaat
     1981 cagaaatagg tgcttctctt ttttgtaaaa ataaaagtat gtaaataaaa tttaaccatt
     2041 ataattttct tttaacaaat gtatacaata aacttgttgt ataattaaga tagataaaat
     2101 aaagggaggg ataatatgaa tttaatagat aatcatattc atactaattt ttcaagtgat
     2161 ggaaaggatt ccatggaaga tacaataaaa aaagctataa gtattggtgt tagatatttg
     2221 acttttacag accatttaga acatgatgaa gatagaggat tttgcataaa ttataatgac
     2281 tatgttccag tatttaataa gtttaaagaa aaatataaaa aggacataga gttattacta
     2341 ggtgttgaag tgggttacag aaaacatcta aaaaatgaaa tagaagaaat aataaattct
     2401 aatccttttg attttgtatt gtgctcaact catacaattg acaatgtacc tgttccatca
     2461 aaggagtatt ttaaaggtct tagcaaagaa gatgcgtatt ataaatattt taatagcata
     2521 cttgaaacta atcgtgaatt tggagattac aatatatatg gtcatttgga ttatatatct
     2581 aggtatggaa tatattcaga taatagagtt atatacaatg actttaaaga tgttattgat
     2641 gaggtcttaa agtcaataat caataatggg agtggtatag agctaaatac ttctggttat
     2701 agatatggac taaatgctat acatccaaat gaagatattc taaaaagata taaagaactt
     2761 ggaggattta tagttactgt aggttctgat tctcataggg tagaagatat atgcaaagat
     2821 tttgatgtgg cctatgatat gcttaagtat cttgacttta aatatgtatc tttatttaaa
     2881 gaaagagaaa cttattttgt aaatatagaa aaagttaaat ctaacaatat agcttagagt
     2941 gtgttttgaa gtaaattaat ggatagttaa aaagttaata gttataatat tgtatcctct
     3001 agaaaaatac tagaggtttt tattttgcta aaataactaa atagaattaa atttaaatag
     3061 tatagtttat tttaaatt
//
LOCUS       DAEOSP010000283         3076 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.287, whole genome shotgun sequence.
ACCESSION   DAEOSP010000283 DAEOSP010000000
VERSION     DAEOSP010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.287"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            160..954
                     /locus_tag="KRH54_003429"
     CDS             160..954
                     /locus_tag="KRH54_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBG8581058.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            955..3015
                     /locus_tag="KRH54_003430"
     CDS             955..3015
                     /locus_tag="KRH54_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBG8581059.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLKVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPALCEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         1181 a          318 c          506 g         1071 t
ORIGIN      
        1 aaaagatatt ataaacgaat aaaaaataat aatttgccag ataaacttgt aaatttttat
       61 gaataatcca ttctaaaaca aaaaactgtg tataaaaaag gtttatatgt tatatttata
      121 taaatataac atatgaacga gaaaggagtg gtttgataaa tgattgaggt agataaattg
      181 tgtaagtctt ttacaagggt tgtaaaagat gataagaata aaagttcaat aagaaagcta
      241 aagaaagtaa aaactaaaaa agaagaattt tttgctgtaa ataatgtttc atttaaagtt
      301 atggaaggtg aaattgttgg aatattaggt ccaaatggtg caggaaagac tacattactt
      361 agaatgttag gtggcatatt aacaccaaca tcaggaagta taaatatttc tggatatgat
      421 tattcaatag atagaaattc agcaaaaaaa gaaataggtt atctctctgg aaatacaaaa
      481 ctatatggaa ggctttctcc gagagaactt ctaacaactt ttgcaagtct atatgagatg
      541 tcaaaagaag atatagaaga atcaattgaa aatattgtaa aaataatgga tatgagtgaa
      601 tttatagata atagaattga aaatctatca actggtcaga ctcaaagaac atctatagct
      661 cgttgtttaa tacattctcc aaaagtatat atttttgatg agccaacact aggtctggat
      721 gttttaagta gtgcatctat tatagatttt atgaagaatg aaaaactaag gggaaaaact
      781 gttctttact caactcatta tatggaagaa gcagaaactt tatgtgacaa gatttttatg
      841 attcacaatg ggaaaataat tgcatcagga acaccacaga gtttaaaaga ggaatctgga
      901 gtaaataatt tgagagatgt attcataaaa cttgccagaa aggaggacat ttaaatgaga
      961 agtaagattg taaaatacat atttaaaaaa gaaatgttag acatattgag agataaaaag
     1021 actttattta tgatgattgt tgtgccactt cttttatatc ctttacttat gttattaatg
     1081 attcaagtaa tgaatatgtc tgcaagtagt atgaatagta aggacattaa tatagctttt
     1141 aataataaac ctaataaggt tttagtatcc atgattgaag atgataattt agaaaaagat
     1201 actaaaaata cttcaaatga taaaggcaaa ataagagtgt tagatgtaga taactataaa
     1261 aagtcattag aagaaaataa aattgatgct tatataaaga tggaagaaaa aaaatcatct
     1321 ataaattatc aaatatatat aaattcttct caagaaaatt cttctaatgc actaaaaaga
     1381 attgaaactg ttttggataa atataaaaga tttaccatag aaaaaacttt atctgaaaaa
     1441 ggattagatg tacaagctac tttagaacca ataacatata gcactgtaga tgttgcaaag
     1501 acggaagaag tggcaggata ctttttaggt cagatacttc catttatatt aataatagga
     1561 gttttactag gttcaatata tccagcaata gatgttatgg ctggagaaaa ggaaagaggt
     1621 acattagaaa cattatttac actgccaata tcaaacttag aactggttat gggaaaatat
     1681 atggcagttt ctcttagtgc aatagtaaca gcaattttaa acattttatc tatgggactg
     1741 actatggcat ttattttagt tagtggaggt attagtagcc aatttggctt tggaaacttt
     1801 aattatggag acttagtctt accaattata actactttaa tttgcatatg tcttttttca
     1861 atggtagttt cagcaataag tatgtgtgta tgttcattag caaaaagttt taaagatgct
     1921 caaaactaca taacacctct tatgttaatt attatgatac catcatatgt ttctatgata
     1981 ccaaatatag tattagatag agtgacagct gtaatacctg tagtaaatat atctttactt
     2041 ataaaaagtg tattatcttt taaaagcgat ttaaatttaa tagccttagt tttagcatca
     2101 aatgtagcct ttgttatatt ggcaatcata ctactgtcta agatgttcaa ttctgaggaa
     2161 atactttttg gtaatagtaa gagtttctct ttcttagaga aaagaagtaa tataaaaaaa
     2221 gggtctatac caactgtaag tgatggtgtt attttatatg cagtaggatt agttctattg
     2281 atttatgttg gttctttagt tcaaattaaa tttggtatgg caggtattat aatgacacaa
     2341 ttcatgataa tttctttacc gttattattt gcatactata ttaaagctga ttttaaagag
     2401 gtatttagct tgaaagttcc caaattaaag catgtgtttg ggagcattgt tctttggatt
     2461 ggtggataca tacttattat gattataaca cagctaattt tattcttatt tcctcaaaat
     2521 atggaagttg ctgaaagttt aaataaggct ctttttatca aagatagtgt tttactgaat
     2581 ttactagcaa tagctttatt accagcttta tgtgaagaaa tgttttttag gggatttata
     2641 ttttcttcat ttagtaaatc aaaagataaa aataagtcta tcaagctagc tataatatgc
     2701 agtggggtac tatttggtat tatgcatatg gattttatta gaataattcc aacatcaata
     2761 ttaggtatta tatttgcata tagtgtgtat aaatctggtt caatatttgt atcaatgtta
     2821 ttgcattttt taaataatag tgtagcagtt gtgttaaatc attatacaac tggaaatata
     2881 accaacttat ataaatttgt tgaagttgat ttttcgaact taaatgtact taaatttgcc
     2941 ttaattcttc taataagtct tatattaatt atgttaggat taagagtttt agatagaaag
     3001 tctttaagaa attaagtata aagttattat atagattaaa atattaataa gcactaaagt
     3061 atatggatat atcttg
//
LOCUS       DAEOSP010000284         3033 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.288, whole genome shotgun sequence.
ACCESSION   DAEOSP010000284 DAEOSP010000000
VERSION     DAEOSP010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.288"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            169..1314
                     /locus_tag="KRH54_003431"
     CDS             169..1314
                     /locus_tag="KRH54_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBG8581060.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVIVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            complement(1428..2804)
                     /locus_tag="KRH54_003432"
     CDS             complement(1428..2804)
                     /locus_tag="KRH54_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBG8581061.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     regulatory      complement(2898..3000)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         1233 a          481 c          390 g          929 t
ORIGIN      
        1 tttagaaatc tataaaatgc aaaggtatca gaaatataaa ggtataaata taattgatat
       61 ttactttcaa attacacata attataaaag aatatatagt tcgatttgaa taaacaatgt
      121 ataataaaaa atagttgact ataattatta aaatataagg agtaaaatat gaatacattt
      181 gaacaattaa aaataagttc cactctaata gatggactca aaaaacaaga cataacttct
      241 ccaacagaag ttcagtcact tgttataggg aatataattc aaaataagga tttattgatt
      301 aattcacaga ctggaacagg aaaaacacta gcataccttt tacctatatt tgaaaaaatt
      361 gatacatcaa aaagagaaac acaagctctt atacttgcac ctacgcatga attagtaatg
      421 cagattacaa accaggttga attattggct aaaaatgcag aactctccgt tacttcttta
      481 gctttaattg gggaggtcaa tatacaaaaa caaataaaaa atataaaagc agtaaaacca
      541 cacatagtaa ttggctcttg tggaagagtt ttagatttga taaaacaaaa gaagctaaaa
      601 tcacataata taaaaactat tgtcttagat gaagtggaca atctattgaa tggaaaaaat
      661 ataacttgta tagaagacat aataagaaca acgttaagag atagacaaat tataggatgc
      721 tctgcaagtc ttactgatag cactataaaa atttgtgata aacttatgaa ggagtttgaa
      781 ataataaaaa caaaggaaaa atcacaaata aatcctaaca tcaatcactc atatttatta
      841 ggtgagataa gggataaatt tacttttctt agaaaggcat tagcggctac aaatccaaaa
      901 aaagctattg tatttgtaaa taatgaaaaa aatatagaag tcatagtatc aaaactaaat
      961 tatcataatt acaaagcaat aggaattttt ggaaatatgg aaaaagaaga tagaaagaat
     1021 gcaataaata aatttaaact tggtaaagca aagattttaa ttacaactga tttatctgct
     1081 cgtggactag atattgtgga tgtaagtcat gtttttaact tggattttcc aaaaagtaaa
     1141 aatgaatacc tgcatagatg tggtcgaact gctagaggaa atcgaagtgg taacacaatc
     1201 tcaataataa caaaaaaaga attagatatt ataaaagatt tgcaaaaaga gttcaatata
     1261 gtaattacac ctaaaacttt acaaaatgga gaactaatag acattattaa ataatacaat
     1321 aaatacaaaa atacaacaaa tacaaaagag ctgtagttta tcatcacaat tgataataaa
     1381 ttacagccct ttattttata ttaaattaaa attttaaata atacctatta tagtagccaa
     1441 agttttatta taaataatat tgccagaaca atcattacta cagaaacatg ttttttgtct
     1501 tctgcctttt tagctccaaa tatattataa aatatattta cgaaaacata agttaatata
     1561 cctaaagtta aaccatcacc tatactatat gttaatgcca tacaagcaat agtaacaaaa
     1621 gctggaacac cttcagttat attatcaaac tctatatcct taactgtacc aagcattaaa
     1681 tatccaacat atattaaagc tggtgctgta gcacatgatg gaattgctat aaatacagga
     1741 gagaaaaaca ttgcagctaa gaataaaatt ccaactgtta ttgctgtcca tccagttctt
     1801 ccacctgcga taactcctgt tgaactctca acataagttg taactgtaga tactccaagt
     1861 cctgcaccaa ctgtagttgc aaccgcatct gtaagtaagg ctctacctac attaggaaca
     1921 ttcccttttt catctaacat gtttgcttta gaacaaacac caactaatgt acctactgta
     1981 tcaaaaaaat caacaaataa aaatgtacaa actactacta aaaaaccacc tatatttgtt
     2041 ggatgtagta catatcctaa atctatttta cctgctattg gtgctaaact ttcgaattta
     2101 aataatccac caggtaaata tatacctaat ttttgtgcat actctggatt tataaaagca
     2161 aatgcccaag caagtaatga actcactact attccaaaaa gtatagaacc tttaactctt
     2221 tttttatcta atacagcaat aattattaat cctacacaag ttattattac tgctggtgta
     2281 aatctaccca ttttgataag agtggcttgg tctccaataa caattccact gttaactaga
     2341 cctataagtg caataaatat tcctatacca cctgtaacag ctaatttcat attttttggt
     2401 atcgcattta caacagcctc acgaactcca cttactgata atactataaa aataatacct
     2461 tctacaaata ctgctgtaag agctacttcc caaggaacac ccatttgtaa aactactgta
     2521 tatgcaaaaa atgcattaag acccatacct gatgctaaag caaaaggaag gttagcaact
     2581 acacccatta aaatacatcc aaatgctgct gctaaacaag tacctgtaac aagtgcacct
     2641 gcaggcatgc ctgtctcaga taaaatattt ggatttacag ctattatata agccatagtt
     2701 aaaaaagtag ttactcctgc aattatttcc ttcttcatgt cgacattttt attagtcaaa
     2761 attggaaaaa gtttctgcat agtactactg tttgttgttt gcataacaac acctcctatt
     2821 aaattttaca aaaaaacgac acagtagttg ataagctgag ccgtatcctt cataattcag
     2881 ttaattaaat atttaattaa ctaaataata gtatacatag tcagaatatt tacggtattc
     2941 cggtagaaac ttatgaccca tattgtcaaa attatatgtt atacagtaca aaacatgaat
     3001 ttttctttac gaatattatt ttaatttata taa
//
LOCUS       DAEOSP010000285         3012 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.289, whole genome shotgun sequence.
ACCESSION   DAEOSP010000285 DAEOSP010000000
VERSION     DAEOSP010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.289"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            273..1550
                     /gene="hflX"
                     /locus_tag="KRH54_003433"
     CDS             273..1550
                     /gene="hflX"
                     /locus_tag="KRH54_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBG8581062.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVELASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRYKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            1783..2280
                     /locus_tag="KRH54_003434"
     CDS             1783..2280
                     /locus_tag="KRH54_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBG8581063.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIEIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEAEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            2329..2853
                     /locus_tag="KRH54_003435"
     CDS             2329..2853
                     /locus_tag="KRH54_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBG8581064.1"
                     /translation="MKSVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELKKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
BASE COUNT         1250 a          259 c          530 g          973 t
ORIGIN      
        1 atccttctta tgatataagt agcttgtttt gtattactta gatttgtaag gagttttttt
       61 attgaaaaaa ctgcataata tatttgggag gaggtgtaag aaagtgattt tttatataag
      121 attgagaaaa taatgcacga ttatggttat tataatagta aataagaagt gtcatttaag
      181 gataagataa aaagcaaaat tcttgatgaa tttaattata gagccataat ctcgtgctat
      241 agattaaata aaaaatagca gataaggaga atatggatat ggcgaaaaaa ggaataacag
      301 taggattaaa cataaataat aaatctgagg actttaatga actaatgatt gaacttgaaa
      361 acttatgttc agcatgtgat attgatgttg tagggagtat aactcaaaat gcaaagcagg
      421 ttaatagagc attttacatt gggacaggta aagttgagga aatattaaac ttaataaaga
      481 aagaaaatat agagatagtt atattttaca atgaactttc tacatcacaa ctaaaaaatc
      541 ttgaagaaaa gcttaattgt gaaattatag atagaacggc attgatactt gatatatttg
      601 cacaaagggc aaaaactaga gaagctaaac ttcaagtaga attagcaagt ttaaaatata
      661 tgttaccaag acttataggt tcaaatgaaa atttaggacg tcaaagtggt ggagttggta
      721 ctaaaaatag aggttctggt gagaaaaaat tagaacttga ccgtagaaga atagaagaaa
      781 agataacatc tttaaatagg gagttggatg atttaaaatt ccaaagagaa actcaaagaa
      841 gtatgagaag aaaatcaaat cttccaaatg tagctctagt tgggtataca aatgctggta
      901 aatcttcaat aatgaataaa ctagtagata tatttaaaaa ctctgaagaa aagaaagtgt
      961 ttgagaaaaa catgttattt gctacacttg aaacttctgt cagaaatata gttcttgcaa
     1021 ataacaaaga atttttatta tctgatactg ttggttttgt aagtaatcta ccacatgatt
     1081 tagtaaaagc atttaggtca acattagaag aagcatgtga ggcagacgta ctgttacatg
     1141 ttattgatat ttcaaatcca agctataaaa gtcatattaa agtgacagaa gatactttaa
     1201 aacaaatagg tgcagatggt atacctatga tacatgtgta taataaaatt gatttaatag
     1261 atgtagaagt attggataga attttagata gtatcgataa agaaggtata tttgtttcag
     1321 ttaaaaaaga tataaatata gataaaatga taaaatgtat ttgtgatagc atatttaaag
     1381 attatgtaag atataagttc ttaattccat atgataaagg tcatgtagtt tcttatttta
     1441 acgaaaatac gagcattata aatacagaat acagagaaga tggagctatt ttagatgtag
     1501 aatgtagcaa tatagaatat aataagtata aaaagtatgc tttagagtaa attttgaaaa
     1561 acttattata gcctaaaata tatttaattt ataatttatt ataaattgat tgtaaatatg
     1621 attttatgaa acggtatagc cataatgcta tgccgttttg ccatttataa taaaaataaa
     1681 tttattgaca taatagtaac taattactat aatataaagt aattagttac tatttaaaat
     1741 tatcaaaaaa ttaaaatcta agataaagag aagtgtataa taatggatgc agaaagttta
     1801 atgaaaaaat atatggtaga tgatgatagt agaaaacaag ctatatttgc aagtatattt
     1861 gtaatgcaaa ataaacttca aactacatgt gataaattag acccagaatt aacaatgaaa
     1921 caatggttac ttttagctat tgcggactct gtagatgaaa ctcttacatt aaccaaactt
     1981 ggagaattaa tggggtgttc aagacaaaat gtaaaaaaac tagctattgc tttagaaaag
     2041 aaaggtttta tagaaattga gcaaaatgaa aaagattcaa gagctatctg tcttgtgaca
     2101 aaagatagag tgcaagagta ttctaaaaaa gtgagaggta tgcaggaaaa agtattacag
     2161 cttttatttg aagattttac agagaaagaa gcggaacagt tattttttgg aattatgaaa
     2221 ttaagtatag gaattgaaaa tgtgaaaaat tatgtagaaa ataatgacgt tggagaataa
     2281 gcaagtatca aatcaacttt gtatgacatc agagagggga gaatttatat gaaaagtgta
     2341 gtagtgattt ataagagtaa gtatggttca acaaaaaaat atgctaaatg gctttcagaa
     2401 ttattgtctt gtgatatttt tgaaactaag aatattaata tagaaaaggt tatgaagtat
     2461 gatacgatta ttttaggagg aggtctttat gcttgtaata tagcgggaat ttctttctta
     2521 aaaaaaaatt ataaagaatt gaagaaaaaa aatttagtag tatttggcgt aggagctgct
     2581 ccatatgatg atgaagtaat aaagaaatta agagaagtaa actttaagga tgaattaaac
     2641 aaaattcctt gcttttattg tcgtggggca tgggatgaga caaaaatgtc ttttatagat
     2701 tatactcttt gtagtttttt taaaaaatct atagctaaaa aagaacgtca aggtcttaaa
     2761 ccagcagaag cacctttatt gtctggagaa gcctttgatt ggtgtaataa agaaaattta
     2821 actccaatac ttgattatat aagagaactt taagaggtat atatttgtaa ttatttatta
     2881 tctaaagtta gatttcatag aaatttacta gacattattt ataaaataat attattaaag
     2941 atggataatt taattgtttc aataaggtta taattttaaa aaatgtttat tttaaaataa
     3001 attattttat aa
//
LOCUS       DAEOSP010000286         3004 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.290, whole genome shotgun sequence.
ACCESSION   DAEOSP010000286 DAEOSP010000000
VERSION     DAEOSP010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.290"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            116..2179
                     /locus_tag="KRH54_003436"
     CDS             116..2179
                     /locus_tag="KRH54_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBG8581065.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(2396..2962)
                     /locus_tag="KRH54_003437"
     CDS             complement(2396..2962)
                     /locus_tag="KRH54_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBG8581066.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
BASE COUNT         1307 a          298 c          443 g          956 t
ORIGIN      
        1 tataaaaagg tatataataa ggctttcata tacatttatg agtcaaaatt ttgatataat
       61 atatagatac atggagttat gaatggaatt tatttaactt atagggggag ttaatatgaa
      121 agcaggagaa attgaatttc ttagttattt agaggggtca aacaaaagtt ttgtagtacc
      181 tgtatatcaa agaaattata actggaagaa agaacaatgt aaaagattgt ttgatgattt
      241 agaggatata atagattctg gatttagaac tcatttctta gggtcagttg tatctatata
      301 tagtatggga aaagaatatt taataataga tggacaacaa agagtgacta ctgtgtcaat
      361 attattgatt gccatgtgtc aaataataaa agagtttggg gaatcaaaag gaattattac
      421 aacagaagaa cagattaagg agtgttatct tataaacaag cactcaagag ataaagatgg
      481 aaaaataaag ttaaaaccaa tagcagaaga tagaaaagcg tactatgaac tatttgaaga
      541 agaaatgaat acagatagaa attctaatat agtttcaaat tatttatatt tttataatag
      601 aataaaagaa gataatatat caatagatga tttgtttgat gctatacaga aattaactat
      661 agtagaaata gagttaaaaa atggtgaaga tgaccctcaa ttaatctttg aaagtctaaa
      721 ttcaactggc ttagatttaa gtgaagctga tagagtaaga aactttgtgt taatgggtag
      781 aagctctaag ttacaagagg aattttataa taattattgg tataaaattg aaaaaaatac
      841 aagtagcaac gtaagtagtt ttataagaga ctatttgact ataaaggaga gaagtatacc
      901 gaataaaaat aaagtatatt ttagttttaa aaggtatgta tcagataatg gtatagatat
      961 agaaatttta ttaaaagatt tacttaaatt ttctaactat tatagagaga tatcttattc
     1021 atgtactgat gataatgatg taaacaaagt tttgaaaagg ataaacaaac ttgaaatatt
     1081 tgtatcatat ccgtttatat tagaattatt tgatgattat aatgaaggta taataaataa
     1141 aaaagtactt gtagaatccc ttaatttagt agaatgtttt atattaagaa gaatagtatg
     1201 tgatgtacct cctaatgcac tgaataaagt atttatgaac ttaggaaggg aaattaaaaa
     1261 atttagtgat tataaagaaa actattctaa tatattaaga tatattttta taaataagaa
     1321 atcatctcaa agatttccta ctgacagtga attttcaaat gcttttgaaa ctaaagatat
     1381 atataatact aagtcaaaga ataagttata tatacttgag aggttagaga attataataa
     1441 taaagaaatt gttgatttag aaaatttaat caataataat atgctaaata tagagcatat
     1501 aatgccacag acattaacaa ataaatggaa ggaatcacta ggagataact ataaggagat
     1561 acatgataaa tatttacata caattggaaa tttaactttg acaggatata attcaaaact
     1621 aagtaataaa acatttgaag aaaagaaaga aatggaagat ggatttaaag atagtagact
     1681 ttatctaaat aaatatattt catctataga taagtggaat gaagaagaaa taaaaaatag
     1741 aactaagata ttactagata gagctgtaga aatttggaaa tatccaagtg ttacatatga
     1801 acctccaagt aacagtgaaa atatatttac gttagaggat gatgatgtta attttacaaa
     1861 tgaaagtata gtatcattta gtatattagg agagtcatat aaggctaaaa attggacaga
     1921 attttatgaa caagttgctc ttactttata tgatttggat tctgtaaggt ttaacagtat
     1981 tgtagacaaa acttataaaa gagattttat agataatatt aagtctagag atgcatataa
     2041 attaagaaaa ggtattaaaa tatcagaaaa tatatattta gaaacaaatt taaatacaga
     2101 ggctaaatta aatataatta gatttttact taatgagttt gatatagagt taaatgaagt
     2161 aatattttcc ataaaataat gaaatatatg cattttttgc gtaatagaat attaattata
     2221 ttaaaaataa aatagcacct cttgatatag atttatagct caaatttata tcaagaggtt
     2281 acgtttaatt aaagtaatac aaatactatc taattaaatt attaataaaa atgttatata
     2341 aaaatgagta tatagcattt taaagagtaa taaaccttac ataataaatg atttattaat
     2401 atgaactaga agtattttta ctatattgtt ttataaaacc tttaggcatt ttacttataa
     2461 aatataccac cataatacaa gacaaaggtt tgtctactat attactaaga atccttggaa
     2521 agaacgcagc agtaaatatc ttctgaccag actgaactaa aaaaccaacc aataaatcag
     2581 caccacttcc acttaaacca ccaaacaata aaactgaaat aggagtacca attaaaggac
     2641 aaacaataga taaaattact cctgtaataa tagcttcttt ataaccaaaa ccttttttct
     2701 tagaaatata tccaacaata agaccaagaa caatatttac aagggcaaaa ggtatatttg
     2761 taggacctga tataacacct aaaactaagt ttgtacatcc accaataaga gcaccccaaa
     2821 aaggacctaa tacgacagcc ccaagtacag taccaactgt atctaaaaat aataatggta
     2881 tatttatcat agatataacc atacctaaga caatattaat aactatgcac attgaagaaa
     2941 atgtgacttt tttagtatcc aattataata cctcctaaaa aaatatatac atttatcata
     3001 gtat
//
LOCUS       DAEOSP010000287         2990 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.291, whole genome shotgun sequence.
ACCESSION   DAEOSP010000287 DAEOSP010000000
VERSION     DAEOSP010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.291"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            45..623
                     /locus_tag="KRH54_003438"
     CDS             45..623
                     /locus_tag="KRH54_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBG8581067.1"
                     /translation="MAKSFTEIEKDNIRKRLISECELNWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIERHNLKLKIEKEKVFGVCNA
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            914..2164
                     /locus_tag="KRH54_003439"
     CDS             914..2164
                     /locus_tag="KRH54_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBG8581068.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILVVGFITLLIAFVSKITLRNENL"
     gene            2325..2894
                     /locus_tag="KRH54_003440"
     CDS             2325..2894
                     /locus_tag="KRH54_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBG8581069.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         1109 a          392 c          448 g         1041 t
ORIGIN      
        1 ttaaagtcat atatatcata tatagataga aaggattgat aaacatggct aaaagtttta
       61 cagaaataga aaaagacaat ataagaaaaa gactaatttc agaatgtgaa ctcaactggt
      121 ctaaatatgg atacaaaaaa actaatattg atgaactttg ttcaaaatca ggaattgcaa
      181 agggttcttt ctatacattt tttgattcaa aagaaaagtt attttttgaa gtattaaatg
      241 gaatccaaaa cagacttata tcatcagtag atgaaatttt aagtaacatg cctccaaaag
      301 aaggctttgc aaaatcctta aaaatgttat atcgattata tgataaaaat ccatttttat
      361 attcaccaaa taatcctgat ttcatcagcc taataaataa actacctcaa gatatgtttt
      421 tacaacttga gtccaataac atacaaagtt tttacgactt aattgagaga cacaatctca
      481 agttgaagat agaaaaagaa aaagtttttg gtgtttgtaa tgctctacta tcaagtctat
      541 tggtaaaaga aaatattgaa tataactata ttgaagtgtt tgactttatg gtggataatc
      601 ttgtagaaca catacttgaa taacaattta aaataatttt tctaagtatt tttttatact
      661 aaataggatt aatatgattg aattaataca aaaaataaac aatataattt ataaaataaa
      721 tgataaacta aatatcacac aaaacttata gaccctagag caaacccatt ccactctact
      781 ctaagccatc tataatagtt tgtatatttc taataatatt agatattaaa gtaagaaaaa
      841 aattccttat aataattgct attatatcaa ttattataaa tattttaata atcttagata
      901 ggtaggtaca tatatggtaa aatcctcgaa cacttttaat aaaaacttca acttgatgat
      961 attaggtcaa ataatctcat tgtttggtgc atcaatactt aaatttgctt tatctttata
     1021 catacttgat ataactggta aagcagaaat atttgctact atactagcag tatcttcaat
     1081 cccaattata gtattctccc ctattggtgg agctattgcg gatatattca acagaagaaa
     1141 cttaatggtt atttttgatt tttcaagtag tcttacagta ctaatcttag cagtattctt
     1201 attcaacaat aatggttcaa tattacttgt aggaattatt atgaccatat tatcagtcat
     1261 aagcacaatg tatcaaccaa ctgttcagtc aagtacaccc cttttagttg ataatgagca
     1321 tttaatgaat gcaaatggta ttgtagctgg agttgcatct cttacaaata ttgcaggacc
     1381 tgtattagga ggtgtcttgt atggtgtgat tggaattaat gcaattatag ttatcagttg
     1441 tgtaagtttc tttctatctg caattatgga gatatttata cagattcctt ttatcaaaca
     1501 agagcaaaac agcaacatta taaaaactat atttactgat ataaaggatg gtattgttca
     1561 tatatgtaaa gaaaatagat ttatactaaa aatacttttt ttagctatag caataaactt
     1621 atttttaagc tctatgttaa tagttggtat cccctatata gtaaaaatca ctatgggagc
     1681 tagtaacttc ttatatggtc tagcagaagg agcaatttct tttagttcac tggtagctgc
     1741 tgttagtatt ggtattttct caaaacatct aaaaatcact aatttgcata tttcctttat
     1801 tatatgtgca gttatgttca taccaatggc ggtttcagta tatccattta tattaaacac
     1861 aggatttcca gttccatttt taatctttat agtatcatct atgctaatat tttttacaac
     1921 gactcttatc tcaatatatg ttataacagt cacacaatta aaaactccaa atgaactctt
     1981 aggaaaagta atgtcaattc tttttgcagg aacagcaatt gcaatgcccc ttggtcaggt
     2041 tatgtatggt aaactttttg atttattatc agatagagtg tacatcatta tactagtagt
     2101 tggatttatt acattactta tagcatttgt atcaaagata acattgagga acgaaaatct
     2161 ttagatacaa agaatttaga tataaagatt taattttcac taatttctga atttaaatgc
     2221 aaaattgtgt atcagtatgt tgacgtttta gaaattgatt gatataatca atggaaaata
     2281 aactttataa aaattcaaaa tattcagcta gaggagtgat aaaaatgagt tacatgccaa
     2341 ctttagaaca agcatgggaa atattaagaa aatacaataa ggaagaattt catataagac
     2401 atgctcaaat tgtatctgga gttatgagat actatgcaaa ggagtacgac cctgaaagag
     2461 tagaattttg ggaaatagtt ggacttttac atgatttaga ttttgaaatg tatcctgatg
     2521 aacattgcgt taaacaaaga gaattaatga cagagcttga tttagataaa tcaataatcg
     2581 attctacaat aagtcatgga tatggattaa ctggttcaga tgttaaacca gagcaattta
     2641 tggagaaagt attatttgct gtggatgaac ttactggttt aattggtgct gctgcaataa
     2701 tgcgtccttc aaagagcgtt tctgatttag aattaaaatc tgttaagaag aaatttaaag
     2761 ataaatcatt tgctgctggc tgttctcgag acgttataag aagtggtgca gaaatgcttg
     2821 gatgggaact tgataagctt atccaaacaa ctatagaagc tatgagaagc ttgattccag
     2881 agatggcaat atagctcctc catacatata ccatagaagt tacatcttta aagagcattt
     2941 ctctttttga tgcaacttct ttttatattt tgtagaataa aaagtttaat
//
LOCUS       DAEOSP010000288         2912 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.292, whole genome shotgun sequence.
ACCESSION   DAEOSP010000288 DAEOSP010000000
VERSION     DAEOSP010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.292"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     regulatory      18..111
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            194..679
                     /locus_tag="KRH54_003441"
     CDS             194..679
                     /locus_tag="KRH54_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBG8581070.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     gene            1034..1432
                     /locus_tag="KRH54_003442"
     CDS             1034..1432
                     /locus_tag="KRH54_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581071.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNTRRHNIL
                     TKLILSIIVIFLVIVLVVLIFIKRKKKKFY"
     misc_binding    1868..2131
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2282..2833
                     /locus_tag="KRH54_003443"
     CDS             2282..2833
                     /locus_tag="KRH54_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBG8581072.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT         1260 a          262 c          432 g          958 t
ORIGIN      
        1 ttcaaattat gttattattc agatataggc aatattagat aaatctaatg acgcaaaact
       61 atagggacta aaatctttat gtaataaagg tcatgtcagc cagttgccaa aaagataata
      121 attctttttg ttaaaagtat ttttagagtt atatatttta ataacttaat atttggcaag
      181 aggagggctt aagatgatat ctgaattaat gttaaaaaat tttcgtaaat ctagcatcaa
      241 ttttaagact aaaattaaat tgaatttaag agaaaaacca gacataaata gtttgaaatt
      301 aaaaagtata ccagaaggta aaattgttaa gttaaagtgt gtagatggaa tatgggcaga
      361 agttgagagt aattatgaca aaggttggtt actttataaa tatttagaaa gattaagtaa
      421 tgctaaaaac aatagtaatg atacaataaa aaatagaaaa aagaattaca taggaaatat
      481 aagaacaaat ggattaagtt tagaacttag aaatgataga acattagaat ctaaagttat
      541 aactacaatc cctgatggtt ttaaagttga agtatgttat agcgttggaa aatgggcaag
      601 agttaatatc aataaaaatg aaaaacgata ttctggatat gtatataatc aatacataga
      661 agagcattta agtttataat acgatactag caagctttaa atatattaat aaatcgcaaa
      721 aaagaatccc attaatggga ttcttttttg tagaaacatt ataacagaac taaagagaat
      781 aaattaacta atttattttt attataaagt aattataaat ggtaaattta attgaaagta
      841 gatttaactt atactttata caccaagtat cataaattaa tactactttg aaaaatctta
      901 aaaaaagtaa aagttatgat aataaaaata atattaaaga taataagata tataagaaac
      961 ttgaaaaatt tgtaattttt agttatatat atattataat gaactagtta aaaattttta
     1021 tgagggggaa aatatgaata agaagaaaaa attgattggg atagctatat cttttctact
     1081 tataatcagc attataaatc cattacaagt agaagcagaa gctattattg agtctagtgt
     1141 atacatttct tcaataaaag agaattatac atatgataat actaaattta aaactattaa
     1201 atctaaaagt agacataaaa gtaggaaata taataaaagt aaagtaaagt tgaaaaaacg
     1261 aagtataaga actaaaaagt atggatatca aactataaat aaatataaaa gacaaaacac
     1321 aagaagacat aatatcctaa caaaattaat attgtccata atagtcatat ttttggttat
     1381 agttttagta gtgctaatat ttattaaaag aaagaagaaa aaattttact aaaaataatt
     1441 aaaagaatat cattttttta atcattggta tctatttaat aatatgatgt aattaaaatg
     1501 gagtaatatt ctgtttcaaa ggaggcatat aaatacagga atgagtatta aatgatggtt
     1561 aattaagtaa atttattgat aaatgtactt aattaacttt gaagaattta attcctcatg
     1621 aatcaaaata taaaattaca tcaacagtta ttcacaaaat taatataaga agaattagag
     1681 tcaaagagca gactattaga ccattaaagt aattattcat agaattttgt atatgaaaag
     1741 aaatgtatag tataatttac tttttttata aaaaatagat tacaaaatat attttttata
     1801 aaaaggtatt gacatttttt agaaaaaaga atatcattat tttaacaaca aaattcaata
     1861 caataaatta aatcctatga caaggaagag tagttaaatt taggttcagt atagagagct
     1921 gaggatggtg gaatcttagc aagaaaaaat ttgatgaatg gcccttagag gaataaattg
     1981 aaatcatagt tttgagagta gactttatcg tcagtcgcac gttatagcga ataaggtatg
     2041 taagtacctg ataatgagat gtatgaaaat tgaattttat attaattttt atataattta
     2101 ggtggcaacg cggataatct ccgtcctatt agtttaggac ggagtttttt attttactga
     2161 aaatttaaaa gatagaaggt ggtgagaaag atgttatgaa tttcaaaagc taaagatgat
     2221 gatatagtag gttggcaaaa aaataaattg aaaattaaaa aaataggagg aagtacaaaa
     2281 aatgaaaaca aatacaaaaa aattaacttt aaatgcaata cttttagcga tgggattatt
     2341 aatacatcaa atcacaccag ctataggatt accaatgaag ccagatgtac cactagcaat
     2401 gttatttgta atattggttt taaatagaga tgattataaa acttgtttga tagcgggtat
     2461 agtaacagga atatttacag cacttacatc aagttttccg ggaggtcaaa ttcctaatgt
     2521 aatagataaa actcttacag caaatatagt atttttatta atgaatatta gttataatat
     2581 gccttttata aaaaaattag ctaagaaaac acaagatact gtagtagttg caataataat
     2641 gccaatagga actcttgtaa gtggaactat ctttttatta gcagctcaag ctatagttgg
     2701 tcttccaggg gcttcattta cagctttatt cttagcagta gtattaccag ctgttttaat
     2761 aaatctagtt gcaggaatat ttttattcaa gattgttagt ctatcaataa gaagagttag
     2821 ttatcaagct taaacacgaa ataatatttt actttataaa gcattataat aactaaataa
     2881 cataagaata aagtagatta taactatttt at
//
LOCUS       DAEOSP010000289         2871 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.293, whole genome shotgun sequence.
ACCESSION   DAEOSP010000289 DAEOSP010000000
VERSION     DAEOSP010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.293"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            333..2144
                     /locus_tag="KRH54_003444"
     CDS             333..2144
                     /locus_tag="KRH54_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG8581073.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVRKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2232..2813)
                     /locus_tag="KRH54_003445"
     CDS             complement(2232..2813)
                     /locus_tag="KRH54_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581074.1"
                     /translation="MLYLLKINQFEKSYDIGIFKNEQSIYKFIAKIPFVKKDNSNFNY
                     TNYYMEFEDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT         1077 a          303 c          401 g         1090 t
ORIGIN      
        1 tagattatgt atatgccaaa tttaaaaaga gacataaaga cattatacaa atatattatt
       61 ggggtgatat tttataagaa aagaagtgat gcagtaaatt aattgagcaa tttaaaaatg
      121 atttaatgat agtgttgatg aatgtttaaa aagaaatttt agaaaaacgt atattgctgt
      181 ttgtgttgga ggtgatgaat ttgtataatt tataggatat aaatgattaa actatttaag
      241 ataaatacat atcactgtaa aatattgata atcatttttt ataagagatt gattatttgc
      301 aaattatata tattacaaat gaggtaacaa taatgaagaa aaaatatgat tttttaattt
      361 attctgctat ttggctagtt attattattc tttttatatt ttctctatat cacagtatag
      421 agcatattaa aattataagt cacactggta ctattcgcag tgaaactcaa aaggttgtta
      481 agcaagaatt gaataatgaa agaaatgatg acttgataaa acgactggat aatattctga
      541 taaagctaag aacaggtaat ggagaaaatg gatttcaaag atgtgataat aaagaatttc
      601 aacaaaaact aaatcaaatg gattctatgt gggaatctat gaagaaagaa attataaaag
      661 ttagaaatgg tgcttcaggt gataaattat ataaattgag tgaagagtat tctgtgttgt
      721 ctaatcaaat tgtttttata tctgaaaaac actctaatgc aaaattgtat tcttttgcta
      781 ctgcactatt tatttatctt atattctcaa caataagtct tttaatttgg gaatattata
      841 ataaaaaaag gtttaaaaga atattttata ctgataattt aacaaaaata aaaaatcagg
      901 ttgcatttga aaacagagcg attgaaattt tgcacaatgc atctaacaaa gagtatgtac
      961 tgctaaatat tgatattgac aattttaaat atattaatga tacacatgga tatgaatatg
     1021 gagataaagt tctaataata gttgcagctg cactttctaa aacttttaat atcaaagaaa
     1081 cttgtgcaag aattggttca gataattttg taatacttgc aaaatataga gattctcttt
     1141 tagaggatat tcgggaaatg ctaactaatg ctataatctc tgaattagat atgaatgtaa
     1201 ctcaaactat atcttattgt ataggagcgt acttagttga aattgataat ttaggttata
     1261 aatccatcaa ttctataatg gataaggcaa atattgcaca caaggtaagt aaaacaagag
     1321 gaatttcctc cacagtatgg tataatgaaa atctgttaaa acagttacaa atggaaaata
     1381 gtatttataa ttatatgtat aaagcgatag aaaatgaaga atttcatatg tatttgcaac
     1441 ctaaatttca aatatcttct ttaaatgttg taagcgcaga agctcttgta agatggtttt
     1501 ctccagaact tggtttttta tcaccagatg aatttattcc attgtttgaa aaaagtggat
     1561 ttattattga attggacttt tatatgttaa aaaaagcgtg ttcatttgta agaaaaacgt
     1621 ttatgaaaaa aaatcaatat acatatccga ttgcagttaa tttttcaagg gttactattt
     1681 accaaaatag tttttatcaa aggtttttag atactgttaa agaatatgaa attcctttta
     1741 agtatataga gatagaggta actgaaagtg cttttaatga aatatctcaa ccagttattt
     1801 caattttaga ggaacttaaa aagttaggat ttttaatttc aatggatgat tttggttcag
     1861 gatactcttc attaagtctt ttatgttctc tatctattaa tggattgaag ttagataaaa
     1921 gcttacttaa agaaaccttt aaccgggaga aagtatatag tattattcaa tgtattattg
     1981 aaatgtcaca tcgtataggc atgtctgttg tatgtgaagg tattgaaact aaaaaagact
     2041 tggaattttt aaatacagtt aaatgtgatg taggacaagg gttttatttt tctaaaccga
     2101 tagaagaaaa agaatttttt aacaaatatg taactaaaaa ataatataat tatttttatc
     2161 agtctataca ttgagtcatt ttatatctcg ttaaatgact caatgtactg ttatgtattt
     2221 taaaatataa attatttaaa acgtttttta tacttatata gaaattcttc tatatttttt
     2281 gattgttctc ttatttcaac tgcttctata tctagtaaat aaagtataga atcatttgta
     2341 agttcaacat attcaccatc ttctgaaccg agtgcatgtc tctcaatttt taatcctttt
     2401 tcttcataat atttttttgt ttctatatat aatgattctc gcttttgtat gtagtctttc
     2461 acttcattat ttggaaagtt atatgaatcc actaatgttt cgccatttat aaaagttttc
     2521 tcggtagcaa ctttttcatc ccaacaatgg atttcttccc aagtaaaaaa tatctcagtt
     2581 ccatctggaa tgaatgaaaa cctggtaata atatataaat aattattgaa atttacttca
     2641 taatattcag gaatatcttc aaattccata tagtaatttg tataattaaa attactatta
     2701 tcttttttta caaagggtat ttttgcaata aatttatata ttgattgttc atttttaaaa
     2761 attcctatat cataactttt ttcaaattga ttaattttta ataaatacaa cattctttct
     2821 ccttttattc aaaatataat ttttataaat ttactaatct ttagcttttt t
//
LOCUS       DAEOSP010000290         2869 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.294, whole genome shotgun sequence.
ACCESSION   DAEOSP010000290 DAEOSP010000000
VERSION     DAEOSP010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.294"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            19..525
                     /locus_tag="KRH54_003446"
     CDS             19..525
                     /locus_tag="KRH54_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBG8581075.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            653..1669
                     /locus_tag="KRH54_003447"
     CDS             653..1669
                     /locus_tag="KRH54_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBG8581076.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2023..2727
                     /locus_tag="KRH54_003448"
     CDS             2023..2727
                     /locus_tag="KRH54_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBG8581077.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         1212 a          271 c          518 g          868 t
ORIGIN      
        1 ataataagga gaagagatat gaaagagtat gtagttgtgg atttagaaac tacaggatta
       61 gatccatata aaggttgtga aataatagaa ataggaataa cagaaataaa aaatgaacaa
      121 attgtaaaaa actattcaag gttgataaaa cctaagggga taatttcttc atttataaca
      181 gaattgacac atataagtaa tgaaatggta gaaaatgaag aaccactaga attagtttta
      241 cctagattta gaaaatacat aggtgataga actataattg cacacaatgc gaaatttgat
      301 ttaaaatttt taaattacta tcttagaatg ttagatttag aaccaataaa caattatata
      361 tgtacagtag agttattaaa aaaatgtaaa agttataaag gaaaaaacaa gaaacttgaa
      421 acagcttgtg cttattacaa catagagaat ataaatgctc atagagctga cagtgataca
      481 ttagcaactg caaaactatt tttgaaaata aaagatgaat attaattata aatatagaga
      541 aaattagtga aaatatcctt tataaaattg acccttaagc ttacaatttt gttctttgat
      601 ttattttgta gaacaatgta taatgtacct tatatgaaga agggagacta aaatgggtta
      661 ttacaatgat aatgataata ataaaataag acgtaaaaaa attagtaaca acaaagatag
      721 taataaaaaa tctaaacatt cttatacttc ggcaaataaa aaaaataaaa gtcctaatac
      781 aagttccagt aaaagaatga gtgaaaaagt agattcacag aatcttataa aaagagatag
      841 agatagtttt acaagagata atgtaagacc tttaaataca gattacaatc aaagagaaag
      901 aagaagagtc gagtataaac aaagagagaa aaaattaaaa agtaaaaaaa gaaaaaaaca
      961 aaaaaggatt agaatagtta gggcattatt atctattatg ttagttgcac taattatatt
     1021 ttcagtggca ggtgcaatat ttacagtttc agctataaaa gggtcaccag aagttactaa
     1081 aaagttaatt aggtctaaat atataagtag tgaggttgta agtataaaac aaatgccaaa
     1141 tgatttaaaa catgcaatag tttccataga agatgagaga ttttataagc ataaaggagt
     1201 agatgttatt tctctagtta ggtcagtatt aaacaatgtg tttacagata caacacaagg
     1261 tgcaagtact atagatatgc aagtttcaaa aaacctgctt actagtaatg ataaaactat
     1321 gaagcggaaa ataagggata tgtataatgc taagcagatg aataagatta tgactaagga
     1381 tgaaattctt gaagcatatt taaataatat gtatctaggt aaaaatgcat atggagctgc
     1441 aaagggagct gaagtctact ttggtaaaaa agttagtgaa ttaaatttag ctgagtgtgc
     1501 tatgttagct ggtatgacaa ataatcctgc aaggtatatt gaccatggag aggctaagaa
     1561 gagacaagtg attgtactgt ataagatgca tcagcttggt tatataactg ataaggagta
     1621 tagaatagca aaagctgatg acactccatt taaatcagaa attgattaaa attgtaagaa
     1681 tatagtattt tcaatagttt aaacaaacaa aaatgtaata aaaaaggtga tttgccaccc
     1741 atttgccacc ataattataa tctaatggca aattgtttta atgtacaaac tagataatag
     1801 aatattgtct taaatgctat attatgctaa aatttaattg tattttgtaa attaaaaaac
     1861 ttataattta gttttctagg gttccgcatt ttataatggt caggtccaag agaaaacgcg
     1921 caattgttgt gtacacggaa ggataaaagc ctgggagata tttaagaata tttctatagg
     1981 cttttttatt tttacatatt ttaaatttga ggaggttttc taatgaaaaa atttgttatt
     2041 gaggatgcat tttggaatct atttcctagt gcaaaaattg gagttgttat ttgtaatgat
     2101 atagataact ctataaaaga tgaagactat aagaaaataa ttttgaaggg agaagaagaa
     2161 gcattaaaat acttggagga ctcagaattt agcaacaacc aagtgattaa agtatggaga
     2221 gaagcattta aaaaatttaa aacgaaaaaa ggagcaaggt cttctattga agcattatta
     2281 aaaagagttc ataatggaaa taatatagga actattaacc ctttagtgga tatctacaat
     2341 tttatctcat taaaatatgc attaccttgt ggaggggagg atatagataa atttatagga
     2401 gatattaggc taactagagc aattggtaat gaggagttta tcccactagg gacagatgaa
     2461 aattcatttc catatgaagg agaaataatt tataaagatg atggtggagc aatttgtaga
     2521 tgttggaatt ggcgtgaagc agtgagaaca atgcttactg aagatactaa caatgccttt
     2581 ttatgcattg aattagtaga tgaaagtaga tttaaagaat ttgaaaatgc tttaaatgaa
     2641 ttagctaaaa tagttcaaga aaaattgggt gggaattgta aaatttcaat tttagatatt
     2701 gataataaag aggtatcagt ggattaatat ggaggtgctg ttgtaatgga ctattctagt
     2761 tataaagata cttatattta gggtaagaag gtctatttta gatataacga ttatcaaagg
     2821 atataatata gaaaataaaa atgtagaata taaaaagcct ttaaaataa
//
LOCUS       DAEOSP010000291         2832 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.295, whole genome shotgun sequence.
ACCESSION   DAEOSP010000291 DAEOSP010000000
VERSION     DAEOSP010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.295"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            303..806
                     /locus_tag="KRH54_003449"
     CDS             303..806
                     /locus_tag="KRH54_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBG8581078.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGVYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            853..1812
                     /gene="moaA"
                     /locus_tag="KRH54_003450"
     CDS             853..1812
                     /gene="moaA"
                     /locus_tag="KRH54_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBG8581079.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSITRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            1816..2286
                     /gene="moaC"
                     /locus_tag="KRH54_003451"
     CDS             1816..2286
                     /gene="moaC"
                     /locus_tag="KRH54_003451"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBG8581080.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            2322..2753
                     /locus_tag="KRH54_003452"
     CDS             2322..2753
                     /locus_tag="KRH54_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBG8581081.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         1184 a          251 c          541 g          856 t
ORIGIN      
        1 taaaaatttt atttgtagag aaaaatatgg acaatgttat aataattata gaaattaaaa
       61 gtacttacat tcattatgta tgtgacatgt ataattagtt ttatatttaa tacagttttg
      121 aagtaatatc ttcattttga gaacgttgga ataataaaaa atgtattatt ataggtgata
      181 taaataaaaa ataaatataa tatgttatat tgctgataga aaagagaata aaagaaaata
      241 ggttttatta aaagagattt ttatttatag gacttaatca atgtatagag gggtgagaac
      301 atatgtttaa tgttgcaatt ataacactta gtgataaagg atatgaagga aaaagagaag
      361 atataacagg aaaaaagtta actgaatttg ttgaaaatac aggagtttat aaagttactg
      421 aatatgtact tataaaagat gataaggaaa tgctaaagga aaatattata agactttgta
      481 atagtaataa aatagattta attcttacaa atggaggtac tggtttttca aaaagagata
      541 taaccccaga agcaactaag gaagtgttgg aaaaagaaat accaggtctg agcgaatata
      601 tgagaatgaa atccacagaa attactaaaa aggccatact aagtaggggt gtgagtggaa
      661 taagaaataa ttctataata ataaatttac ctggaagtcc taaaggtgca gttgaaaatc
      721 taagttttat aattgatgtt ttagaccatg gcatagaagt tttaagagga gaagctacag
      781 aatgtgccac taaaaaaagt gaatagtata taatctagat agataaaaaa tagacaaaga
      841 ggtagataaa gtatgattga taagtatgga agaaaaatag actaccttcg tatttcactt
      901 acagataagt gtaatttaag gtgtgtctat tgtatgccac cagatgtaaa atttgataaa
      961 aattatataa atgaaaatct aagctttgaa gactacaagt ttattataaa ggctatggct
     1021 gaacaaggta taacaaaaat tagatttact ggaggagaac ctcttttata tcctaaatta
     1081 aatgagctta taaaatttac aaaagaagaa tgtggaataa ataacatagg aatgaccact
     1141 aatgctattg gtctttctga tagaattttt gagcttgaaa aatgtggctt aaataaagtt
     1201 aatataagtt tagattcttt gaaggaatat aagtataaat ccataacaag aggaggaaac
     1261 ttaaaagatg tcttaaaatc gattgaatct tgtttaaatt taggtataaa agtaaagtta
     1321 aattgcgttg ctataagtgg gtttaatgat gatgagctta aagattttat gtttttaaca
     1381 gttaaatcac caattgatgt aagatttata gagcttatgc cattaggtga ggctaataga
     1441 gtttataaga agggttattt taatgtaaaa gaaatgattg aaaaaatata tggtttatat
     1501 aaagtcaaaa atgatgaaaa aagtacagca gaatattata tgtttaaagg agctaaagga
     1561 agaataggaa ttataactcc attaagctgt tcattttgta acacttgtaa tcgtattaga
     1621 cttacttcta gtggtgcaat aaaattatgt ctacattcaa aagaagaaat agatataaaa
     1681 ccatttttac ataaaccatt gttatttaga gaatctatga aagatataat aaaacaaaaa
     1741 ccagaaaaac atcatttaat agaaaataaa tgtagtgata caaataggcg tatgtatgaa
     1801 attggaggat aagtaatgtt aacacatata aatgaaaaag ggtatgccaa gatggtggac
     1861 ataagtgaaa aagatgatac taaaagaacg gccgtagcta cagctacaat tactcttagt
     1921 aaggaagcat tagaaaaagt aaaaaatggt cagataaaaa aaggagacgt attgtctgta
     1981 gctcaagttg caggtataat gggagctaaa aatacatctt caaacatacc aatgtgccat
     2041 cagattaaat tagttggatg tgatttagaa tttgatgtag atgattctat gtctgctatt
     2101 aagatatacg caacctgtaa atctcttggg aaaactggag tagaaatgga agctttaaat
     2161 gcatgttctt tagcagcact tacaatttat gatatgtgta aagcagttga taaaaaaatg
     2221 gttataagta atatccatct tgtggagaag actggtggaa aatcaggaga ttttaagttc
     2281 gagtaaatta ataatatatt gggttaaaat ttggaggaaa tatggcgaag gtagtgtcaa
     2341 taaatataag tgagaaaaag ggtgtaatta aagttcctgt acaatgtgca gagctaaaaa
     2401 taaatcatgg gatagttgga gatgcacatg caggaaactg gcatcgtcag ataagcttac
     2461 ttggaaaaga aagcatagat aaaatgaaaa tgaaaggctt tgaccaatta aaatatggag
     2521 attttgctga aaatgtaact acagaaggga tagaagtttt ttccctgcct gttggtacaa
     2581 agttaagaat agggcaatgt gaagttgaag taactcaaat agggaaaaaa tgtcacaatg
     2641 gatgtgaaat taagaaattg acaggtgact gtgtgatgcc acgagaagga atttttgtaa
     2701 aggttctaaa agaaggaatt ataaatgtga atgattcaat agatgtaatc taatgaagtc
     2761 actattaaaa tataattata agtaaatact attaagattt aaatatatat tttaagagag
     2821 aattaatttt aa
//
LOCUS       DAEOSP010000292         2811 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.296, whole genome shotgun sequence.
ACCESSION   DAEOSP010000292 DAEOSP010000000
VERSION     DAEOSP010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.296"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            433..1470
                     /locus_tag="KRH54_003453"
     CDS             433..1470
                     /locus_tag="KRH54_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581082.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNQILTKVVNDDNEIEEKIIEEDLLISENTIKLDDKEAIINDKNIELCKKEVQVE
                     SEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAINL
                     YTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKSEW
                     SYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYDEY
                     FKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTKVG
                     RNFIRYSLRSI"
     gene            1581..2723
                     /locus_tag="KRH54_003454"
     CDS             1581..2723
                     /locus_tag="KRH54_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBG8581083.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         1268 a          228 c          491 g          824 t
ORIGIN      
        1 ttataattat tattgaatat atttttacat ataagatata aggtgataga tagaaattgt
       61 tggattatgt aaataattaa tatataatac cattatgggc aataaataca aatcaaatga
      121 taatatagtc tatttatgta aatgttatat tgtatcctaa gtataatagt aagttttttg
      181 atagtggtgt agaaagtaaa aatctactag cctaaattga tgtatggatt agtttaggaa
      241 cagtaattgc atatcttaag tttgatgtca tgttgggcta ctagaaatag gtcatctttt
      301 tcacttgaat atcttttctt taaacatggg gagtatcata tcaaaaacat atgaaaatta
      361 agtgatttat gagaacagta atattttata tgatagtata gaatagtaat aaagtattag
      421 gaggaacatt gagtgttaaa aaagtggttt ggtattgtga aaaaaagaca aaaaagtgag
      481 tcagtaaaag aagaaggtga agtaatatta aaaaacgaaa aaatattatc tgaagaaaag
      541 ttgatagatg aagaaggagt tgtagttaat attgataatc aaatattaac taaagtagta
      601 aatgatgaca atgaaataga agaaaaaata atagaagaag atttgttaat tagtgaaaat
      661 accataaaat tagatgataa agaagcaatt attaatgata aaaacataga attatgtaaa
      721 aaagaagttc aagttgaaag tgaaaaaata ggtttaaata agtttgaagg actggaccaa
      781 aatgaaaatt ataatctaga aaaaaatgtt attgaagaaa aaaaagtaag tgaatgtttg
      841 acagaagaag atttagagta cataaaagaa attaaaataa aaagaggaaa aagtataaaa
      901 gctataaact tgtatactaa agaagagtgg gtttttgaca ctcatataca gtgtagtaaa
      961 aaactcaaag ttccattagg gtacataaga gaaaatttaa aatatggata tatggattac
     1021 tttggagatg caataaatta tttaagtgaa gtattaaata tagatgaata ctgcaaaagt
     1081 gaatggagct atctcgataa tagcaaatct ccatctgaga tatttaatat tctaaacaat
     1141 aaaatattta gtataaggct ttctaatgaa aaaagaaatg aaattttgac aaatgataaa
     1201 attgaagcat taaaaatgaa ttatagattt gaatgtattg atgaagaata tgatgaatat
     1261 tttaaaaaat ataagtctat aatcaaaaga ggtggaaaga aaaaagttga attagtaaat
     1321 aaaaaaggtg acattttaga aatatttaag tctttagaag aatgtgccat ttatttgcaa
     1381 aaggaaaaga atgaagttat acaaatgtta aaatatggag atacaaaagt aggaagaaac
     1441 tttataaggt atagtttgag aagtatttaa tttcatttta aattcataag agtttcattt
     1501 ctgattcaca catttaatat tattttaatg taaaataata aaggtgaagt attgcacaat
     1561 taaaattaag gagggtatta atgtctgaag aaaaaaatcc aaaagaaaat acattagatg
     1621 aatctaaaaa ttcagataat gtaaattcta aaaaagatga tgtgtcagat gaaaaaaata
     1681 ctgataacaa agttaaaaaa agggaaataa agctaaacaa caaaacactt aaagctattg
     1741 tagcaattgt tgcagtagga gctatatgtt taggtggagg ctatgttttt ggcaaagaag
     1801 ctggaagaaa attgcctgct acttcaaaac actataatag cagtgaggta gtagctacta
     1861 ttggggataa aaaaataaca ggtgaaatgt taagaaagag aatggagcca ttattttatt
     1921 taaatgcaaa aactactatg actgatgacg aaatacgttc atatgaatca aatatgattg
     1981 aaactatgat tacatctgat atattatcct ctgaagcagc aaaagagaaa ataagtataa
     2041 ctaatgacca actagatgct cagtataata atttaatgag tagtatagaa tcagcaatgg
     2101 gaatgactaa agaagagttt ttaaaacagt ttgatttgac tgaaaaatat ataaaagatg
     2161 atatgaaaaa agagctagta gcatcaaaat atttaaatga aaaatcaaaa gttactgata
     2221 aagaagcaga gaactattac aataaaaaca agagcaatta tttacaagtt agagcttcac
     2281 atattttaat aaaaactgtt gatgataaag gtaagcaagt tagtagtagc aaaaaagctg
     2341 aacttaagaa agaagctgaa gaaatactta aaaaagccca agctggggaa gattttgcta
     2401 ctctagctaa aaaatactct gaagatagta gtgcagaatc aggtggagat ttaggtttct
     2461 ttggtaaagg tcagatggtg gaatcttttg aaaaagcagc ctttgcattg aagaagggag
     2521 aggtatctaa caaacttgta gaaagtgatt atggatatca tataattaag aaaactgatg
     2581 aaaaatatca gccatttgaa gaggtaaaat cagatttagt aagcagttta acatcagaaa
     2641 aacaaaacct acttatacaa aatctaaaag aaaaatataa tgttaaaatt acagataaat
     2701 atcaaatgaa agctaaagaa tagataaagt caatttatta aaaaaaagaa aaattaactg
     2761 tatggataaa agctattttt actctcaata tagagagtat aaaatagctt t
//
LOCUS       DAEOSP010000293         2746 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.297, whole genome shotgun sequence.
ACCESSION   DAEOSP010000293 DAEOSP010000000
VERSION     DAEOSP010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.297"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            294..2429
                     /gene="rnr"
                     /locus_tag="KRH54_003455"
     CDS             294..2429
                     /gene="rnr"
                     /locus_tag="KRH54_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBG8581084.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1197 a          260 c          504 g          785 t
ORIGIN      
        1 atcctatttg atggtgtata attaggtgat aaagagtaat ttaagcaatt atgataaaga
       61 ataacttaag taattataat gatatttaca aataatttat aaaacataat aatttgtttg
      121 aagttagtat gacaaagctt acattaaatc aaaaatataa ataagtaatt ttaacttgta
      181 ataataataa ggaaatatag aaaattatgt gataagtaaa aaatgttttg tctgaagaaa
      241 tataaaagac aatataaata ataatataat gaaaagtaaa acaggaggga aaaatgatac
      301 caggacttaa agaaagtttg ctaggattaa taaatgaaag tgcatataat cctctaaaaa
      361 aagaagaatt agcagaaata tttgatatac attcatcaga aatgccaatg ttttataatt
      421 ttttaaatga attagaagaa gatggttata tctgctttac taaaaaagga aaaatagtat
      481 ctcccaatca aatgggatac tttgttggaa aatttgtatc acacaaaaag ggttttggat
      541 ttgtagaatc tgatgttgaa tatacacagg atttatttat ctcaagtgac aatataaatg
      601 gtgctatgca taatgataga gttatggcag aaatagtagt acctgcaact gaagaaaaga
      661 gagcagaagg aagaatagta aaagtaataa agagagaaat aactagaata gtaggtacct
      721 tccaatcaag taaaactttt ggatttgtaa caccagacaa caagaaattt actaaagata
      781 tatatatacc taaaaaacat tttagtggag cagtagataa tgataaggta gtatgtgaaa
      841 ttacagtatg gccacaagag gatagaaaac cggaaggcaa aattatagag atactaggtc
      901 aaaaaggtga aagaggcgta gaaatagatt ctataataag agaacatggt cttccagaag
      961 aatttccaaa gaaagtattg caagaagcag aagctgtagc tgttgagatt cctcaagaag
     1021 agataaaaag aagaagagat ttaagagatt taaacatatt tactatagat ggagatgacg
     1081 caaaagactt agatgatgcc atatcaatag aagtattacc taatggaaac ttcaaactag
     1141 gagttcacat agctgatgtt acacattatg taagagagaa aaataaactt gacaaggaag
     1201 ccttaaaaag agctacatca gtttatttag tagataaagt aattccaatg ttgccaaaaa
     1261 ctctttcaaa tggagtatgt agcttaaatc catttgaaga taagttaact ctttctatat
     1321 ttatggaaat agaccataaa ggaaatgtgg ttaaacatga aatatgtgaa tctgtaataa
     1381 attcaaaagc tagaatgact tatacagaag tatctgatat attagaaaaa gatgatgaaa
     1441 aacttaaaaa gacttttgaa catgttgttg aagattttaa aaatgctgaa attttagcta
     1501 ggattcttat gtcaagaagg gaaaaaagag gagctataga cttcaatttt ccagaggcaa
     1561 agataattct aaatggaaaa ggcgaagttg tagatataaa gccatatgaa agaagaatat
     1621 ctaataagat aatagaagaa tttatgctta taagtaatga gacaattgcc gaacattttt
     1681 actggatggg aataccgttt gtgtatagaa tacatgagac accatctttg gaaaaaatgg
     1741 aagaattgag taagtttata tctacatttg gatatataat aaaaggtgat aaagaggaag
     1801 tacatccaaa agcattacaa ggtataatag agaaaataaa gggcaaaaaa gaagaaggag
     1861 ctatcagtac tataatgctt cgttctttaa aacaagctaa gtactcacca gaatgtagtg
     1921 gacattttgg attagcagct aaattctact gtcactttac ttcaccaata agaagatatc
     1981 ctgaccttca aattcataga ataataaaag aatctttaaa taataaaata agtggaaaaa
     2041 gacaagaaca gcttactact atagtggatt atgcatctgt tcaatcatcc gagaaagaaa
     2101 gaaaggcaga gttagctgaa agagatgtac atgatttcta taaggctctt tatatgaagg
     2161 ataaagttgg tcaagaattt gagggtgttg tatctagcgt aacttcattt ggaatatttg
     2221 ttgagttaga taatactata gaaggattga taagacttgc aaatatgaat gatgattact
     2281 atatttatga tgagagttct tatagtgttt taggagaaag aactaagaag tcatataaga
     2341 taggagacct tgttaagata aaggttgaaa gtgttaatgt agattttaaa gaaattgact
     2401 ttgagatagt tgaaaaaata gaggagtaga taaaacagct aaaataagag ataacaagtt
     2461 gtagtattta tgatatgtat aaatatatag tgcacaatac tctataaaat aaatgataaa
     2521 aataggtaat tgagaaatat ctaatggaaa tagttaactg aaagataaat aattaagaaa
     2581 tatttaatga aagtagctaa ttgaaaaata agtaattgag aaatgtttaa tagaaagagc
     2641 taactgaaag ataagtaatt aagaaacatt taatagaaaa agccaactga aaaataaata
     2701 attaagaaat gtttaataga agtggctaat tgaaaaataa gtaatt
//
LOCUS       DAEOSP010000294         2718 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.298, whole genome shotgun sequence.
ACCESSION   DAEOSP010000294 DAEOSP010000000
VERSION     DAEOSP010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.298"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="KRH54_003456"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="KRH54_003456"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBG8581085.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGTPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMPDGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWHENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKTKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKTQKDKLVSQAKKI
                     ASPSKGKTELEEIRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGASKKG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYSANCYTPEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTRAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1063 a          432 c          550 g          673 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttgatt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gaatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttccacaa gggactgacg aaacagcgaa
      301 tgtacgggtc taaatttgta ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataccg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa gctgacgaag aacaataagc agttgaacca
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaataccct
      601 caagtcgtgt atatattaga aaagtattat gttgttttaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agcaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaagaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaggtaa gatatattgt cgcaggcact ccacacgggt atcgacctaa gccagtaagg
     1021 cgaaaagaaa taccaaaacc aaatgggaaa accagacctc taggcattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tatcaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctacggttt cagacctaat aggtcggttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caaactgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caattaaaat gcctgacggc
     1381 acaataacat atccagagaa aggaactcca caggggggaa ttatatcccc actactagcc
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagtc aatggcatga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gtcgctacaa agaaagtgct gaaaaaacca aaatagcaat tactcaatgg
     1681 atcgagcaac gactaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaagaccc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaag ggaaaaacag aattagagga aataagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacaaa tgtaaatctg
     1981 gattgtatga aacttaatag agctgtaatg gctatcttca ctaacagatt aggtgcaagc
     2041 aaaaagggca gattgagaaa aacaggcaga caacttacaa caaaagaagc aacaagatat
     2101 ggtaaatcta aaatgcttcg atatgttgct ggcgagggcg aacctatatt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaaa gtgtattcag caaactgcta tactccagag
     2221 ggaagaaaat ttatccatac caatctaagt gttgataaat tactgatgta tcagttgatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gcccaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacac
     2401 tgtcatcata ttgtaccaac tagggctggt ggtaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacatcg tctaatacac gcaaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaaactaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatc
//
LOCUS       DAEOSP010000295         2694 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.299, whole genome shotgun sequence.
ACCESSION   DAEOSP010000295 DAEOSP010000000
VERSION     DAEOSP010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.299"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            142..1341
                     /locus_tag="KRH54_003457"
     CDS             142..1341
                     /locus_tag="KRH54_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBG8581086.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            1393..1896
                     /locus_tag="KRH54_003458"
     CDS             1393..1896
                     /locus_tag="KRH54_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8581087.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVVHS"
     gene            2003..2320
                     /locus_tag="KRH54_003459"
     CDS             2003..2320
                     /locus_tag="KRH54_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581088.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            2478..>2694
                     /locus_tag="KRH54_003460"
     CDS             2478..>2694
                     /locus_tag="KRH54_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBG8581089.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATV"
BASE COUNT         1098 a          187 c          502 g          907 t
ORIGIN      
        1 tctatatgtt aaaatatagt agaagatgtt agtaaataat aggatgagtt ctattttaat
       61 acaattaaat taagcttagt ttgaaatttg tatctaaaaa gtaaagtaat ctagaattaa
      121 gaaaatggag atagaattat aatggaaaaa ttatattata caaatcagta tataagagat
      181 tttacagcag aaataattga aataaaagaa gtagaaaata agtatcatgt tttattagat
      241 aagacagctt tttttcctgg tggaggaggt cagtttggag acctgggaaa aatagagaat
      301 gatgatgtaa taaatgtgta tgaagatgga aaaaaaatat accatgtatt agataaaaaa
      361 cctataaaga tacataaggt aaagtgtagt attgattggg atagaagaga agatggaatg
      421 catcaacatt ttggacaaca tgtattgtct ggatgttttt ttaaaacttt taatgcaaat
      481 acgacaggtt ttcatttggg aaatgagtat agtacagtag atatagaagg taatttagaa
      541 gaggataaaa ttagagagat agagttgttt gccaatgaaa ttattcgtca aaatatacaa
      601 gtagagacat tacttccaac gaaaaaagaa ctaaaaaaga tttggttaag acgtgatttg
      661 ccaaatactg atgaagagat aagagtaata aaaataggtg atttagatac gaatgcatgt
      721 tgtggagtgc atccaaaatc aactggtgaa ttgagaatga taaaaataaa gaaatgggaa
      781 aaacataagt caagtacaag aatagagttt ctagcaggta aaagggcagt ggatgaagta
      841 ttgaagaggg atatatattt gactaaaata tgtagatatt taagctgtag tgaggaagat
      901 gctataaatg gtattgtaaa tctaaatagt aaagtggaag aggctttaat taaaaaaaga
      961 aggcttgaag aagtagtatc tacatatcag gtcaaagaaa tgattgaaaa agcaaataag
     1021 gttggcaata tttcggtcat taaaaaaatt tatgatgatg aagatttgaa atatgtaaat
     1081 aagatagcaa ataaaattgc agaatatgaa aatagtattg cattattagc tgttaaattt
     1141 aatgataaaa taaatttagt ttttgcatgt tctaaaaatt taaaagatat taatatggga
     1201 gttttattaa aggatgcaat aagtcttata gatggcaagg gtggtggaag taaagtatta
     1261 gctcaaggag gaggtaagaa taatggaaat ttagaggcta cacttgatta tgcatttatg
     1321 aagatagaaa aaactctata aatatattat ataaaaaacc ttagtagata aaatagatat
     1381 ggaggaataa gtatggagca acaaaaggac tacatagagt tatatgaaaa tgaagaacta
     1441 atagagataa gagaagcacg tatggatgat ttggatacaa tagcaaaatt taattataat
     1501 ttggctaaag aaactgaagg aaaagaactt gatatggatg ttttaactaa aggtgtaaaa
     1561 gcattgttat tagatgagag aaaaggaaaa tatcatgtat atacagtatt tgataaagtt
     1621 gttgcacaga ttatgtatac atatgagtgg agtgattgga gaaatggaaa tttcctttgg
     1681 attcagagtg tatatgtaga taaggaatat agaagaaaag gtatctttaa ttatttattt
     1741 aattatataa aaaatatatg tgataaagat gaaaatatag ttggaatgag attatatgta
     1801 gaaaaagaaa acataaatgc aaaagcaaca tatgagtctt taaatatgta tgagtgtgat
     1861 tataatatgt atgaatacga agtagtacat tcgtaatata aatttgtaat actgttatac
     1921 ttatgttata aagtattgat tatattgacg attatatgaa ataaactgga tattataatt
     1981 agaattttta gggggtataa aaatgggcgt tttaaaagga atgttttatg tatttttatt
     2041 tttagattta gtgtcaatat tctgtttctt tttcaataaa ggaaaaatag catctaataa
     2101 aattgtgttt aatgcaatag gagtacttac atttgtactt tgttttatgc tttttagtta
     2161 ttatcccaat aataatctta ttggaaaatt tattgcttct ctatttttta tatttggagt
     2221 tgcaggagtt atgttgaaag aaaaaaattt tttatatgct agacttcttt taactgtagt
     2281 tatagttttt tctacattaa gattgttcgt tattcaataa aatgtatttg tatgaaaatt
     2341 tattaaagtc tacacattta agtgtaggct tttttgattt tttgtttgaa aattaaggaa
     2401 attttaagga aattttaaaa tcgtcttaaa tatttattga tatacttaaa atattaatag
     2461 cgaaaggaga cttatcagtg atagaaagta aaaagtcaga aaaattgtta ctgtcaatta
     2521 tatacataat atttttggta tatattttaa tgattataag aataatttta tttagagatg
     2581 taccaattta cgctattttc aagggaacgt ttaggaatgt aaatttaatt ccattttata
     2641 caatatatca atttatagta gatagtaatc ttgactttat gaaagctact gtaa
//
LOCUS       DAEOSP010000296         2666 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.300, whole genome shotgun sequence.
ACCESSION   DAEOSP010000296 DAEOSP010000000
VERSION     DAEOSP010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.300"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            68..1192
                     /locus_tag="KRH54_003461"
     CDS             68..1192
                     /locus_tag="KRH54_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HBG8581090.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPGVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANSVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(1666..2490)
                     /locus_tag="KRH54_003462"
     CDS             complement(1666..2490)
                     /locus_tag="KRH54_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581091.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
BASE COUNT          938 a          406 c          293 g         1029 t
ORIGIN      
        1 tattttacaa tctttcttca attgatatat aatatgttta tataaactaa tgtatgtgag
       61 gtgaaatatg agcaaaaaaa caaaatattt ttctttaatt ctctttccta ttgctttgct
      121 tttaaatttt atagccagca aagttccaaa tattgttgaa aaatattact cccaattcat
      181 agataaaata attgtacaaa tattgagtaa agtttctgga attttcccat tttctctcta
      241 tgagattaca atgtacataa tagttatatc tatcttctta tttttatgtt gtactttatc
      301 tacaattttt aataagaaaa aaaaattaaa catatattta aaaaattcca ttttaaatat
      361 attatccatt gtatcaatag tctactttct ttttgtagtg ctttggggaa taaactataa
      421 taggattcca ctagaaacca cacttataaa taactataat ttaaaaaaca atacctctat
      481 ccaatcaaaa caatatagtg ttaaagagct tactaaatta tataaattcc ttgtaattaa
      541 agcaaatgaa actagaaaac tcacattaca agataaaaat ggtattgtaa aatctaatac
      601 agattataat ggtgtcataa atagagctca actaggatac gataatatct tggatatatt
      661 acctggtgtt agtggtagtt attctaagcc taagtatatt atttcatcaa atctaatgtg
      721 ctatacagga ataactggta tatactttcc ttttacaggt gaggcaaatg taaatatagc
      781 aatacctgac ttatatattc cttgtacagt tgaacatgaa atggcacatc aaaggggatt
      841 tgctagtgaa gatgaggcta attttatagc gtatcttact tctataaaac accccaatat
      901 tgattttaac tattctggat atattttagc tttaaattat actgcttctg ctttaagtaa
      961 ggttgattat aatgcatatg ttgatatttc tgctggcatt tcagattctg tccgtagaga
     1021 tttaaaaaat gaaagtgaat tttggcaaaa atatgaaggt aaaattaatg aaatttctaa
     1081 tgagtttaac aattcttact taaaagctaa tagcgttact gaaggtactc agagttatgg
     1141 taaaatggtt gatttattgc taacttatta tgaattatat ccatataact aaacactaca
     1201 attcaaaatt ttattcatat acagctgata ttaaatcagc tgttttctat ttaaataaat
     1261 tatttaactt caagcaatct tatataattt gatagtactt atactatctg tataacattt
     1321 caattctaac tattttagtt aaaacatttc tattatcttt tttaataaat gttttaacta
     1381 aaatttttaa aacataaaac ttccacatta tttcactatc tatataaaaa tctaaaaatt
     1441 cacatataaa attacataac taaaaaatca taaatttata gaaaacatat attcaaaata
     1501 aaaaagcatg tatcaaaact tgacacatgc ttataatctt atgtatatat atcattttgt
     1561 cacaataaaa atttacttgt aacactgata tttttaaaca aaatagtcct tatttaatta
     1621 aattaacatt aattaaattc gtactcattt tgctatcatt aaacattact tatagtaatt
     1681 ttctaataca ctatttgaaa cttccttccc ttgaaaactt ttcaaccaat tctcatatgc
     1741 tgagtttttc catacactac catcattaaa tgttacttgt tttacacaag atataatgta
     1801 ctttacacca tgctctccag ttgattgttt ccatttatca aacaatagaa aggcatcatt
     1861 ttgattctta cctggtgcta ttgcattttg ctttgcatat tctttctcca tttgtttcca
     1921 ttctttcaac caattttcta cccatgcctt gccttgttgt ttttcaaaca tacttattat
     1981 atcctgtggt ggtgattgtt gcatttttct ataagtatgt gaatatgatt ttggagacac
     2041 aggagatatg cctgtaacaa taccatagtc tacaccagct gggctaaatc caacacttcc
     2101 tacttcgcca tctgctgcca cattttgagc atcccaatat agctcaagtg gttttccatt
     2161 tttatcatat gctaagcatg taatttcata atccataatt gccttttcac tgctatttgt
     2221 taagcttata acattgctag gccaatgata tggagtatca tcatactgta ttttgctatt
     2281 atttataaat aatggaatat tctcattata aacaagacct ttactacttt gaatatctga
     2341 taccttcttt gtgacagtac ttaccgtatt tgctaagtat acatttgttt tttctgaatt
     2401 ggtcgtacta agagaaaatg ttgttgtcgt tccagtaatc agcaaagcaa caactaaaat
     2461 aggtaagcta gatatttttt tatatctcac aattcttcac ctctttttct attaacttat
     2521 aataaaacaa tttaccaaaa cattaaaatc tatgttaaca taatttacca actgttctag
     2581 taactatatc ctatcatagt tactatcaag tttcaatact attgatggaa attttcacta
     2641 tattaaatct ttttttaatt ttataa
//
LOCUS       DAEOSP010000297         2655 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.301, whole genome shotgun sequence.
ACCESSION   DAEOSP010000297 DAEOSP010000000
VERSION     DAEOSP010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.301"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..116)
                     /locus_tag="KRH54_003463"
     CDS             complement(<1..116)
                     /locus_tag="KRH54_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8581092.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGS"
     gene            complement(925..1962)
                     /locus_tag="KRH54_003464"
     CDS             complement(925..1962)
                     /locus_tag="KRH54_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBG8581093.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
     gene            complement(2059..>2655)
                     /locus_tag="KRH54_003465"
     CDS             complement(2059..>2655)
                     /locus_tag="KRH54_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8581094.1"
                     /translation="GLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQS
                     LSLIMSSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQ
                     YGDRLIMLHKGEVVLDVKDKEKEDLTVEEILEKFEYAV"
BASE COUNT          880 a          405 c          341 g         1029 t
ORIGIN      
        1 tagctaccat cagcagacat atctggaaaa tctaagattt tgtaagttat ataaaccccc
       61 agtgccataa tcgataaaat taaactttgc gtcattacag atataatacc tgacataaat
      121 aaaacactcc tttgaaaata tttttataat tttaattatt tgctaatttt gtgtataaaa
      181 aaaccacata gtgtacaaaa ctatgtggta aaaacacgaa taagccacat agtttaatct
      241 atgtggctct tatatagtta aatgaaaact ttataaacaa tatagccaac atagatacaa
      301 tcccaaaatt ttagtagtct taaggggtct gtatctatgt taaaacgtag gtacaaaccc
      361 taaaaatagt ggctataata ataataatat tttctattgt ttaataaagt attcataatt
      421 atctctccct tttaatattt taattagtca gaaaattata tgtattctcg acttgttata
      481 aattataaat tatacaataa taaaagtcaa ctgctaaatt taattaaaat actaaataat
      541 gtatactcag aatactttta aattaatgta ttgtttgcaa aaaacgttat cctaagttat
      601 aagtgaatgt ggaaatttta tcaaaatatt tactagatat ggaataacta gacatgtata
      661 tcaatcaaat atcataaaaa aaagctgtct ggagaatatt aaaattcttt tggacagctc
      721 aagtagtgat tagaaagtta tataactttc ataatcagtc gtcaatatag acgactttta
      781 tggaaaatga ataactttat atgcaatatt caaattgcta caataagcac aattttcaaa
      841 gggttagggt ggataagagt acttattata taaagttttc atattaccta cttcatatta
      901 cctatttttt gaaattaata ggttttattt tgcagatttt gcattttcat ctgactttat
      961 atactttata ttatctgctg ataacttttg catgtcaagt gcttttagtg tatcttcgtt
     1021 aattattatt tcagtttcat ctaatgtagt aactggtata tcgccaacag attttccttc
     1081 taatacttca atagcaagtt ctccagcctt ataacctagt ttttcataat ctattccaca
     1141 acaagctaaa gcaccagaag acacagaacc ttcttcagag gctattatag gaatcttgtt
     1201 ttcattagca actttagaaa ctattggcat agaagaaact attaaattgt cagtagggac
     1261 atataaaaca tctattttgc caactaaact agaaatagct tggttaactt cacttgaaga
     1321 gctgattcct ttttcaacta cttcataatt atttttctta gcataatcat gtagagaatc
     1381 aacttggatt tttgaattaa cttcactagt attgtatata acccctattt tctttgcttt
     1441 tggagtcaat gttttaacca attctaatgt tttatcaatt gaaagataat cagatgtacc
     1501 agaaacattt ccacctggtt tttcaagaga tttaacaagt ccagcttcta cagggtctgt
     1561 aacagcagtc attattatcg gaatatcttt agtagcatta taagctgctt gtgcagatgg
     1621 agtagatata gcatatatca aatctttttt gtcggataca aacttactag caatactttg
     1681 tgtagtaggc atatcatttt gtgcattttg gaaatctatt tttatattat ctccatcttt
     1741 atagccttta tcttcgagtg ctttgatgaa tcctttcttt gctttatcca gagatggatg
     1801 ttccactaat tgagtaatac ctatattttt ttgttttttg ctatctgttt ctttattttc
     1861 atttgaagca ttggagccat cattttgaga acatccagta agcattgata caccaagtac
     1921 tcctgctata attaaactta atattttttt actttttatc attttatttt cctcccttaa
     1981 aatttattaa ttatatttgt tcatatattc atatgataga ttataaatat ttctgattaa
     2041 aactaagatt ttaatttctt atactgcata ttcaaatttt tctaagattt cttcaactgt
     2101 taaatcttct ttttctttat cttttacatc taatacaact tcacccttat gaagcattat
     2161 caacctatct ccatattgaa gagcgtgttt caagttgtga gtaaccataa gtgtagttat
     2221 atttttctct cttacgattt tatctgttaa ctctattact tcatttgatg tttttgggtc
     2281 aagtgctgct gtatgttcgt ctagtaataa gaccttagga cttgctaagc tagacataat
     2341 tagtgatagt gattgtcttt gaccccctga aagatattgt actttaatat ctaaatattt
     2401 ttttaaatcc aaagaaattc cttcaagtaa gtgttccaag tcattcgttt tatgacgaag
     2461 acattttttt aagttcaata atttcccttt attaagtgct agagataaat tttctctaac
     2521 cgtcatagaa ggacaagttc caaggcttgg gtcttgaaaa actctactta ctatttgagt
     2581 tcttttatac tcagctaaat ttgttatagt agtactatct aaagtaattt gaccagaaga
     2641 ttcttttata agccc
//
LOCUS       DAEOSP010000298         2508 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.302, whole genome shotgun sequence.
ACCESSION   DAEOSP010000298 DAEOSP010000000
VERSION     DAEOSP010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.302"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            108..842
                     /locus_tag="KRH54_003466"
     CDS             108..842
                     /locus_tag="KRH54_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBG8581095.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFAFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRRSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(985..1476)
                     /locus_tag="KRH54_003467"
     CDS             complement(985..1476)
                     /locus_tag="KRH54_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBG8581096.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            1825..2208
                     /locus_tag="KRH54_003468"
     CDS             1825..2208
                     /locus_tag="KRH54_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581097.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
BASE COUNT          973 a          277 c          369 g          889 t
ORIGIN      
        1 atttatttat agaaactttt attattttat ataaaagtga taaaaactta tattttgtgt
       61 ttagtagagt gttgctaagt gaatatattt aaaatggagg aaactttatg aaacaaaata
      121 aatatgatga tgatagattt tttaataagt acagtaaaat ggaacgttct acaaatggat
      181 tagcaggtgc tggagaatgg catgttttaa aaaaaatgtt acctgatttc aaaggaaaaa
      241 gagttttaga tttaggatgt ggatttggat ggcattgtca atatgcagtt gagaatggag
      301 caatttctgc tgttggagtt gatatttcag aaaaaatgtt aaaagaagct agaaacaaaa
      361 cgcaacttga taatataaat tacatatgta tgcctattga agatattaat tttccaaaag
      421 atagttttga tgtagttatt agttccttgg catttcatta tattcaatca tttgaagata
      481 tatgtaagaa tataagtaat tgtctttcaa ataaaggtaa ttttgcattt tctgttgaac
      541 atcctatatt tactgcatat ggaagtcaag agtggtactg tgataaagat ggcaataatc
      601 tacactggcc agtagataga tattttgaag aaggagtacg taggtcaaat tttctaggag
      661 aagaggttat aaaatatcat aaaacattaa caacttattt aaatacgtta atgaaaacag
      721 gatttgaaat attagaaatt attgaacctc aaccagaaga aaaactgtta gatacaattc
      781 ctgatatgaa agatgagcta cgtagaccta tgatgttact tgtatctgca aaaaaaagat
      841 aagtaaatat aaataatcat tattgtagaa aaaaagttgt agaaaataat tacactaatg
      901 gagatgatta taccagtggt attaattaca ccagtagtaa ggaggcaaaa gcattcgttg
      961 cttttgccat tttttaagtt gcaattatac ttctacgtag tatatttcgt ttccattttc
     1021 ttctataagc tttttcatat cttcaaattt taaaaataca gtagcagtat tatcatttgg
     1081 atgtatacct attttatcca tttttaataa atccttatca aatacaactt ttacaacagc
     1141 ctctttatca tttaatatac caaaaggagt cacagctcct ttagtaagtt tcaagtattt
     1201 ttgaagtctt tcttctgatg caaaactaag aggtttacta tcaatttgtt gacgaatatc
     1261 ttttaaatct gcttttttgt ctttacctaa tacaactaaa aaatgttctt ttcctttagc
     1321 atcacgaaga aataggtttt ttacaacaaa ttccatatgt aatgttttta attcttccat
     1381 ttcttcaatt gtataaactg gtggatgatt tacagcctca taaggtatgc ttaactcatc
     1441 taatttttta tatacttcct gttgttgatt gttcatgatt tcctcccatt agaaatttat
     1501 tttatatata aataatatta tcacagaaaa ctttaaaaga aaacgtttta tataatatag
     1561 acaatagatt atatttttat aaagaagcaa gaatactcaa taaaacttaa agaaattaaa
     1621 caatttctaa aaataccttg aaaatagaac cttatttgcc atttataaat tatataaaaa
     1681 gggattatat aaagtcatat atgaaaaaac tatattatac tatcttttct ttttggaata
     1741 aactatactt taaatataga tatttaattt aaataaattt tacagtatta atagtaaatt
     1801 atcttagaaa ggaatgtgct ttatatgtct acagacaaag ttgttggaat catagatgaa
     1861 gaaacggcag aacttgcagg aattgaatat actggtaaaa tttatgcttc ttctggtgtt
     1921 ataaaacata taaagaaaaa acatagatgt caactgagta aggatatttt taatgatata
     1981 atagatacta taaaaatggt attaaagtca cctgaatata taggaagtca tcctaaaaaa
     2041 cctggaaaaa gtgtggagtt tataaaaaaa attgatgatt atattttagt agctacagaa
     2101 ttagatacaa aaaaaggata tttatatgta tcttcaatgt atgctataaa agaagcaaaa
     2161 ttagaaagta gagtaaacag tggaagagta aaacaatatg atggttaaca taatattatt
     2221 aattttccct aaaatattga aaaatattat tatataagtt ataatattaa tatacacgta
     2281 gatatttatt agttagattt gaggttggaa aaggcaccca atacgctact atagagtagt
     2341 taatagagat gcaggatacg ccgccctgcc aaatctaatt aaattaaaaa gagaggatta
     2401 attcctctct tattgttttt ttgagatact aatttaatct atatagaaag attgaaggtc
     2461 atttatacta aaagttttca gttatataca ttaaactaat taaataag
//
LOCUS       DAEOSP010000299         2490 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.303, whole genome shotgun sequence.
ACCESSION   DAEOSP010000299 DAEOSP010000000
VERSION     DAEOSP010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.303"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(136..2181)
                     /gene="bclA1"
                     /locus_tag="KRH54_003469"
     CDS             complement(136..2181)
                     /gene="bclA1"
                     /locus_tag="KRH54_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HBG8581098.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPVGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGNTGVTGSIGPTGVTGPTGNTGEIGPTGATGPTGVTGSIGPTGATGPTGEI
                     GPTGATGATGVTGSIGPTGATGPTGATGVTGEIGPTGEIGPTGATGPTGVTGSIGPTG
                     ATGEIGPTGEIGPTGATGPTGVTGSIGPTGATGPTGATGEIGPTGATGPTGNTGVTGE
                     IGPTGATGPTGNTGVTGEIGPTGATGPTGVTGEIGPTGNTGATGSIGPTGVTGPTGAT
                     GSIGPTGATGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIG
                     NSSSITVDANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTT
                     EPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATLTIFRIADTVMT"
BASE COUNT          566 a          558 c          381 g          985 t
ORIGIN      
        1 tttaaaattt ttatgaatat attttattat tattgttatt ttattcattc taaaataggt
       61 aaaactcaat atataaaaat acttttgtca tttaaataaa gcctaaatag tttttaagat
      121 tattttagac acgttttaag tcataacagt atcagctatt ctaaatatcg tcaaagtagc
      181 cgcagttcct gctgattgcc catttcgtat ccctactgtt gtagatgaaa aattatttat
      241 tgtaaaagta gtcccaccaa ataaaaaacc agcaaaatat ccagaaagta ctcctccgcc
      301 tcctggctca gtagtaagcc ctgccaaatc ttttcctcct aaaattggga ataggatagc
      361 gaaagaatat tgatttatac ttgatgaacc tggttgtaat gctactgaaa ctgatatata
      421 atacacacca ttttcttgta ctgtaaacgt accatttgca tctactgtta tacttgaact
      481 atttcctatt attgtttgcc aacctgttat agcatctcca ttatttagtg tttgtggaga
      541 tggtgcattt acgaggaagt tagcaactgg ctgagaggaa ttgcctgttg ctcctgttgg
      601 acctgttggg cctgttactc ctgtcgctcc tgttgctccc gttggtccta tacttcctgt
      661 cgctcctgtt ggacctgtta ctcccgttgg ccctatactt cctgtcgctc ctgtatttcc
      721 tgttggccct atttctcctg tcactcctgt tgggcctgtt gctcccgttg gtcctatttc
      781 tcctgtcact cctgtatttc ctgtcggacc cgttgctccc gttggtccta tttctcctgt
      841 tactcctgta tttcctgttg gacctgttgc tcccgttggt cctatttctc ctgtcgctcc
      901 tgttgggcct gttgctcccg ttggtcctat acttcctgtc actcctgttg ggcctgttgc
      961 tcctgttggt cctatttctc ctgttggtcc tatttctcct gtcgctcccg ttggtcctat
     1021 acttcctgtc actcctgttg ggcctgttgc tcccgttggt cctatttctc ctgttggccc
     1081 tatttctcct gtcactcctg tcgctcctgt tggacctgtt gctcctgttg gtcctatact
     1141 tcctgtcact cctgtcgctc ctgttgctcc cgttggtcct atttctcctg ttggtcctgt
     1201 tgctcctgtt ggtcctatac ttcctgtcac tcctgttgga cctgttgctc ccgttggtcc
     1261 tatttctcct gtatttcctg ttgggcctgt tactcctgtt ggtcctatac ttcctgtcac
     1321 tcccgtattt cctgtcgggc ctgttgctcc cgttggtcct atacttcctg tcactcccgt
     1381 atttcctgtc ggacctgtta ctcctgttgg tcctatactt cctgtcgctc ctgtacttcc
     1441 ggttgggcct gttactcccg ttggccctat acttcctgtt gctcctgtat ttccggttgg
     1501 gcctgttggt cctatgcttc ctgtcgctcc tgtacttccg gttgggcctg ttactcccgt
     1561 tggtcctgta cttcctgtcg ctcctcttgg tcccattgga cctgtaattc taattggaag
     1621 acactcacat ttaggataac aattatctat aattctatct tcaaatgtta caagtatata
     1681 cggtttgtta caacctctac tagatgtaaa ttcaagaatc tgataagggt aatttaaaga
     1741 taaagctaac ccataattag gatatttatt cattgcccat gcttttatag ttcccgtaat
     1801 atttaaacaa atatagttgg atgtatcact tatcccaact ggtaaatatc ccctaatatt
     1861 ttccatagac actctatcat tccatgttat agttcgtaaa ttataatatt cacttattct
     1921 tttaatttca atatctactt tttctttaaa aatatgattc ttatttgaat caatatgaat
     1981 aaataactct gcttttaata tacttttgtc ttttaatata tctggtattc tcactttaaa
     2041 aaaagtaatc aacttttctt ttacaaaact atttgaacat ttacttccta ttaagcaata
     2101 atctaaatta ctaaagtttt tatctggata ttttttagat ataaaagtat catcatttaa
     2161 ataaagtata atatttctca tttataatcc ctccattttg ggtctatatc atactatgtt
     2221 taaaatacat gcttagttac aatataatta aaatttatag tattaaattt ttatataata
     2281 atgctgattt ctacaaaaaa agaacatgtc attagaaatt tctaatagca tgttcttttt
     2341 cattattata tattacactt taaattattc cttgactggc ttagctggtg taaactaaac
     2401 cagtctttct aaaatattaa gcactttttt cttattatgt tttttaggat ttattgtcta
     2461 tattattgta aaccaaagtt ataaaattgt
//
LOCUS       DAEOSP010000300         2487 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.304, whole genome shotgun sequence.
ACCESSION   DAEOSP010000300 DAEOSP010000000
VERSION     DAEOSP010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.304"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(225..980)
                     /locus_tag="KRH54_003470"
     CDS             complement(225..980)
                     /locus_tag="KRH54_003470"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBG8581099.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVAEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKSNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1420..2409
                     /locus_tag="KRH54_003471"
     CDS             1420..2409
                     /locus_tag="KRH54_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBG8581100.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSSGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
BASE COUNT          917 a          311 c          366 g          893 t
ORIGIN      
        1 taaataggat aatatttaat aggtttatat gatgaagaga ttataaaata ataaacaata
       61 taattccata taccaataaa taagataatc tttaagaaaa atgattatat ttaaaaggta
      121 aacataaaat ttataaactt ttagaataga aatctcaatc aattagatat tcaaattgat
      181 gaatatgaat acctaaatag attgagattt attatgtaca aaacttatcc taatatatct
      241 gacattttat aaagcccaga ttgcttacca attaaaaatt tagctgcaac tatagaaccg
      301 tttgcaaata cttcatcaga ttgagaatga tggcttaatg taagaacttc attatcacct
      361 gcaaatacaa catcatggtc gctgattata ttgccacctc ttatagagtg tattccaatt
      421 tcatttgatt ttcttttaca atctctacca tatctaccat gtgtattttc actttcaggc
      481 aagacttctc taattgcatt agctatcata gtagcagttc cactaggggc atcttctttt
      541 ctattgtgat gtttttctat gatttcaata tcaaaacctt ctaacatctt tgaagcatcc
      601 ttaactaatt ttattaatat attcactcca agagatgtat ttgatgataa taatatagga
      661 atgttttttg atgcttcttc tattttattt aagtcatttt catcatatcc agtagtagct
      721 aatactacag gtgtattatt ttttagagca tatgacaata ttggctctaa agtctcatga
      781 tgtgaaaagt ctataattac atcagttttt tcagctacat cagtcattgt tttgtaaagc
      841 tttgtatcac tatgatgacc tttttcatca gcagacctag ctacaccaca aaccaactct
      901 aattcattat ctttttttac acattctgct aaaactcttc ccattgttcc attatagcca
      961 ctaattataa ctttcaacat aaattcctcc tatgaaatta ataaaaattt gttatatttc
     1021 tataagttta ttttatcact aatataattt atataaaata atattagtga taataagctt
     1081 acaatttatc ttacattaaa aaatttaagt ggatttttca aaatatagat aaatttcttt
     1141 ttcattgtat aaatgataaa aatatatttg acttcatacc aaaaagagtg ccccatgaac
     1201 taaaaagtta attttgaaat accatattca tatatcatat gatttatttg gaaatcaata
     1261 ttaatacatt aaaataacta aatacatgta tgcataaaga tgtataaaaa atatataaac
     1321 atatagtatt aattttaaat tcaacaattt aattgttaaa agtgttatta ttaaattaga
     1381 aatatcttaa cttaatatac tgagatggga gtgatagata tgttaaaaga agaaaaaact
     1441 tatcaagaga aagacgcaaa agtattcaat ggagtggcag ctttgataat gattttatta
     1501 tttttagtag tttcaatagg tatcatggtt tttgggatag taaaacttga tagtggattt
     1561 actggaatag gttcacttat gttaattttg ggacttgtat ttatagtagt agattttatc
     1621 ctattttttg ggctaaaaat gattaatcct aaagaagcta tagttctagt tttgtttggt
     1681 aactattatg gaactataaa aaaagaaggg tactattggg tgaatccatt ttgttcagca
     1741 ataaacccag cagtatcaag aattaatgta tcgaaatctt catctgatga aaaggtagat
     1801 atatcaacta attcaagtgg caaaaaagta tctttaaaaa caatgactct taacaatgaa
     1861 aagcagaaag tcaatgattt attgggaaat ccaattataa taggagtggt tgtaatttgg
     1921 aaggttatag atgcaacaaa ggcagtattt aatgtggata attataatac attcttatct
     1981 attcagtgtg attctactat tcgcaatgtt tcaagacttt atccatatga tgtatctgaa
     2041 gatggagatg aaaaatctct tcgtggtagt agtcaggaaa tagcagatag attaaaagat
     2101 gaattacaat caagggttga tattgcagga attgaagtgt gtgaagtgag aattactcat
     2161 ctttcttatg caccagagat tgcagcagca atgttacaac gtcaacaagc agaggctata
     2221 atagcagcac gtaaaaagat tgttgagggg gctgtaagta tggtagagat ggcattgact
     2281 aatttaagtg aaaataatgt tgtgactttg gacgaagaac gtaaagcagc tatggtaagt
     2341 aatctgttag ttgtgctttg tggcaataaa gatgcacaac cagtagtaaa cagcagttct
     2401 atttattaaa ttataaaaag tttaatagtt tattttattg atatatagaa aatttagtag
     2461 ttcattttat taaattatag aaagatt
//
LOCUS       DAEOSP010000301         2457 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.305, whole genome shotgun sequence.
ACCESSION   DAEOSP010000301 DAEOSP010000000
VERSION     DAEOSP010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.305"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(22..726)
                     /locus_tag="KRH54_003472"
     CDS             complement(22..726)
                     /locus_tag="KRH54_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8581101.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(817..2178)
                     /locus_tag="KRH54_003473"
     CDS             complement(817..2178)
                     /locus_tag="KRH54_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBG8581102.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
BASE COUNT          818 a          449 c          252 g          938 t
ORIGIN      
        1 ctttttcatt ttatttaaat tttattcgac aacagcattc aaagttaatg tatatccata
       61 gtgatatgtg tgtgagtact caaaaatctc actattttgt aacttacaaa tttggcttat
      121 atataaaagt ggctctggtt cttctaattt tagattatcc atacattctt cattcgatat
      181 tacagcttgt acttctctct ctgacatggc tatcttgtat ccacacactt cttcaacaaa
      241 attataaaga gaattttcac aatgagtctt atttaaattt ggaaataact taactggcat
      301 ataagtaatc atatatgaaa ctcttgcatc attagctaat cttactcttt ttatttccca
      361 aacattttct tctaaactta tattcaactt acttgataaa ttttcatctg cctgaactat
      421 ttcaagagat accaatatat tagatacttt atagccttta ctttccatct ctttagtaaa
      481 tccacttaca gacgatatat tatcatatat attatttgca ataactatac tacctacacc
      541 tctttgtcta gttatatagc cttctttaac tagattatta agtgcagacc ttacagtcat
      601 tctagttaca ttaaattctt cactcagctg tttttcagaa gggattaaat ccccttcttt
      661 caaactgtct gaatttatta actctctaac atgattttct ataactttat aaattggctc
      721 tttcatttaa ttcactcctt attaaatctt ttatataata agataatttt atatacaaac
      781 aaagaaaatg tctatacatt taagataatt taaattttat tcagaaatct tgtacaactt
      841 tccgagtttt aataaagata taaatagact tatcattaat atacctttta atatacttga
      901 taaggttata ctccaccata caccatttag acccaacatt tctggtctag atataaagta
      961 agccaatggt actcttgcac cagtcagtat agtaattata atagaaggta tataggttct
     1021 ccctaatcct ttgaaagctc ctgttgttat tatttctaaa cacataaaaa attgagaata
     1081 gcctaatatt ttaagataaa ctgctccttt ttctattgcc tcactttcat ctataaaaat
     1141 agaaaatata ctctctcctg caaacactaa tactaaagta gttattatac caagtacaga
     1201 agatacagtt atagctattt tgcaaccttt atttattcta gaatacttct tggcaccata
     1261 attttgtcca acaaaactgc ctaatgcaac tgatagccct tcagccgtca tccaggaaat
     1321 tgcttctatt tgtgagccta ctttttgtac tgcaacagcc acaggccccc atgatgctac
     1381 tattactcct aatagcattg agaatactgt aaacatacca ctttgtattg ctataggtaa
     1441 tcccaattta tacagaactt tataatattt agtctctatt tctttaaata gtcttaattt
     1501 aaaatattct tctttacttt taactattat atatataaaa catgtagtaa ctactatctg
     1561 agcaaataca gttgcaatag cagctcctgc aacaccaagt tttggagcaa atccccatcc
     1621 aaaaattaat atagggtcaa gtataatatt agttattaat cctactgtat taatacgaaa
     1681 aggagtttta ctatttccaa gaccattgaa aattgcagta aatactggat taataaaata
     1741 aaataccata cctagagcta ctattatcaa atactgtcta gacatggtta taacttctaa
     1801 gtctcctaat ctaaaaaaac ctatcaattg tttattaagt actattaaag aaattgtata
     1861 tattattgct agtataatat ttatttctat tgctgacttc atatatgatt tagtagtgct
     1921 tatattattt gcaccaatac tttgtgctac ttttatctct ccacctactt ttgaaatcat
     1981 aataaatgcc attgcaagcc atggaaaaaa accagctgtt ccaactgcag ctactgcttt
     2041 acttccggct ttaccaaccc atatcatatc tatcatgtta taccccatct gtataaatga
     2101 agtccccatt attggaagtg atagcttaag taatttttct gtaattcttc cttctgttaa
     2161 attaagtctg tctttcattt tccacctctt ttatattaaa tttatttgct tatatagcta
     2221 gattaatcat tttatttgat tgagttcact atccttcaat ttatattatc atatctcagg
     2281 attgcatctc aatcatactt atgtttaaga tacagtattt tacctatcta tgataaaatt
     2341 aatacttatg aatataaatc tagccatttc atcaatacat attctaacct ttaacttaac
     2401 ttcaattaag tatacatatc gtaaccaatt agaatatttt atacggtatc ttccatt
//
LOCUS       DAEOSP010000302         2427 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.306, whole genome shotgun sequence.
ACCESSION   DAEOSP010000302 DAEOSP010000000
VERSION     DAEOSP010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.306"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            39..638
                     /locus_tag="KRH54_003474"
     CDS             39..638
                     /locus_tag="KRH54_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581103.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            complement(808..1275)
                     /locus_tag="KRH54_003475"
     CDS             complement(808..1275)
                     /locus_tag="KRH54_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581104.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNNDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            1386..2330
                     /locus_tag="KRH54_003476"
     CDS             1386..2330
                     /locus_tag="KRH54_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBG8581105.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
BASE COUNT          924 a          278 c          386 g          839 t
ORIGIN      
        1 attttttata gtataaaaca tctaggaggg taataaaaat gaaattaaaa agaactttat
       61 ttgtaggatt agcactagta ttagctatag gtataactgc atgtactaaa aataatgaaa
      121 aaccaagtga aaatacagga aaaaatccta atgaatctgt aaataataat gaaggatata
      181 tgaattatta ttcaacaagc tataatgatt atatagcagg tctaaataga tattctattt
      241 atgatactcc agaaagtata aataatcagt ttaaagataa agaatatcca ggaaatgaaa
      301 aatatctaag tgatgtaaag gctgcatata aagatagtag agataaaata caatcatttg
      361 taacatctct taaaaaagat ggtaaaacag aagatacaga gttaaagaaa atgaatgatg
      421 acctaatcgc tgaaggggaa agattagtaa aagatataga taagagaata gaaaaattag
      481 ataaggtatc tgatgaagat atgaaaaaag gtcaaaatga ctttataaaa ttagttcatg
      541 gaactgaaga tgttggaaat gatataggta gtggttttag aaaaatgata aaagatatga
      601 atgatagatt aggaataaca ggaaataata agaaataata gaataaatag ttttgaaatt
      661 tgaaataaat tatttgagat atagcgaagc cttataaata atacattttt tggcttcgct
      721 tttttgcatg ttattaagat attaattttt atcttgtaac cttaatattt ttattataat
      781 ttatttagag gttattttca cacgttttta cttgtttttt ctctttttaa atttttgttt
      841 tgcaaaattg taagattctc ctattaaagt tttaaagttc tcaaatgtct tttcagtagg
      901 gcttaggaca catatccatc ccatccatga gtagatagga tgtggcatga ttgtgtccaa
      961 ttttgtaaaa tcaaaatcca tatcaactat ttgacctaca cctggacgct ttggaatata
     1021 tccaaacatt tcaataaaag tttctttttt taatcctata ttcacacggt atacatttgg
     1081 acgactaact aacgaacctt tatcattatt actgtctttt tccttaatgg taagaacata
     1141 tacccctcgc tttaaaacac cattaggatt gtagtaaatt gctcgttccc cccaactatc
     1201 cattagtaca acatcatcta agtttgatag acaataattg agaatatcat cagccttaat
     1261 agtatcattt tccataaagt atcaatccct tctttcttta ctattattat tgctttagat
     1321 gcaagaatat cttatcatat aatagtgaca attattgtca ctatatttaa attaggagta
     1381 taaatatgtc aagagctgag cgtttaattg aactgatgat aacaataaat gcaaaaagaa
     1441 gttttacagc aggagaatta gcagaagaat tttctgtatc aaaacgtact atactaagag
     1501 atttacaggt gctggagagt attggatttc cattatattc aaaagttgga gcggctggtg
     1561 gatatcatgt acttaaagag cgtatacttc caccgattgc attttcagaa agtgaggtaa
     1621 aatctatatt ctttgcatat cagtcactag aatattataa tgatttgcca tttgagcaag
     1681 aaactatttc tgttttaaaa aaatttttaa attgtatacc aaatgatatt caatataata
     1741 ttgagaatat aaggcgtaaa tttgtttttt ggacaccaga taggcactgt tcaacaccat
     1801 tattgaaaga attgtttaat atagtaatga atgaatttac aattaaaatt gaatattcat
     1861 caaagcaaaa aaatagtgta cgtaccattg ttcctattgg tttgtatgcc atgaatggac
     1921 tttggtattg tccagcttac tgtataaaat cagaatctat taaggaattt cgtgtagaca
     1981 gaattgtgaa gattttgagt atagaaaact tatcaagtaa gaaatataag gttttatctt
     2041 ctatacatga ttatcttaag aatatggaag taggtacaga ttatcatatt aaaattaatt
     2101 tgacagatga gggagtaaaa cgttgtgaaa cagaattttt gttggcaaga gggttgaaaa
     2161 tcttgtctaa aggtggatac atagatatgt atataccaaa atctacactt aactgggtgg
     2221 ctgaatattt tttaactttt gggaaaaatg ctacaataat agaacctata gaacttaaac
     2281 atttgataaa gtctaaagta ctagagctat ataatcatca ttgtatttaa attattttat
     2341 ttaatagtag gatagagttt ggataaaatg ttaattttct taatttactt tacagatgat
     2401 ttagatagat tgatgttatt taattta
//
LOCUS       DAEOSP010000303         2393 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.307, whole genome shotgun sequence.
ACCESSION   DAEOSP010000303 DAEOSP010000000
VERSION     DAEOSP010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.307"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..155)
                     /locus_tag="KRH54_003477"
     CDS             complement(<1..155)
                     /locus_tag="KRH54_003477"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBG8581106.1"
                     /translation="MLNINVFWIFLPIFSTTIEWIVLKFLLNEFSELRKNRLTLNSSI
                     VIAIMIIT"
     gene            complement(149..277)
                     /locus_tag="KRH54_003478"
     CDS             complement(149..277)
                     /locus_tag="KRH54_003478"
                     /inference="COORDINATES: protein motif:HMM:NF016296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581107.1"
                     /translation="MEYIQFICKNTVVINGKDIPVSKYRMANLKKKLTYVLGDVLC"
     gene            complement(416..1126)
                     /locus_tag="KRH54_003479"
     CDS             complement(416..1126)
                     /locus_tag="KRH54_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8581108.1"
                     /translation="MYRIVICEDDITQIAFLRECILKSLEGISSQIELFEFNSGEELL
                     ETNLEGIDIFFLDIKMLQLTGMDVAKIIRETNDTSEIIFITSIVDYIQEGYKVRAYRY
                     LLKPIDFGDLNESILSCISDIIKKRENFMLIENKGIINKILINSIMYIEVRKKVLTIH
                     TKNDTYYTKNSMDKIELELEKYNFFRCHKSYLINLEYIQFICKNTVVINDEDVPVSKY
                     RMADLKKKLTYVLGDVLC"
     gene            complement(2217..>2393)
                     /locus_tag="KRH54_003480"
     CDS             complement(2217..>2393)
                     /locus_tag="KRH54_003480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581109.1"
                     /translation="TIVPPDTETKVTLVFTVTHTSSGKTADTGEIEVTVPARTTTVSS
                     LLGRIAVNIFNFNK"
BASE COUNT          923 a          332 c          195 g          943 t
ORIGIN      
        1 gtaattatca taatagcaat tactatgcta ctatttaggg ttaatctatt ctttcttaac
       61 tcactaaatt catttaataa aaattttaat acaatccatt ctatcgtcgt agaaaatata
      121 ggtaaaaaaa tccaaaatac atttatattt aacataatac atctcccaat acatatgtta
      181 attttttctt taaattagcc attctatatt tactaactgg tatatcttta ccattaataa
      241 ctacagtatt tttacatata aattgaatat actccaaatt aattaaatag ctcttgtaac
      301 atttttaaaa atttattctt tcttaaatca ctgaattcat ttgataaaaa ttttaataca
      361 attcattcta tcgccgtaga aaatataggt aaaaaaatcc aaaatacatt tatatctaac
      421 ataatacatc tcccaataca tatgttaatt ttttctttaa atcagccatt ctatatttac
      481 taactggtac atcttcatca ttaataacca cagtattttt acatataaat tgaatatact
      541 ccaaattaat taaatagctc ttatgacatc taaaaaaatt atatttttct agctctaatt
      601 ctattttatc catactattt ttagtataat aagtatcatt tttagtatgt atagtcaaaa
      661 cctttttcct aacctctata tacataatac tattaattaa aattttattg attatacctt
      721 tattttctat cagcataaaa ttttctcttt tttttataat atccgatata caactcaaaa
      781 tactttcatt taaatctcca aaatctatag gctttaataa atatctataa gcccttacct
      841 tatagccttc ctgtatgtaa tcaactattg aagttataaa tattatttca gaagtatcat
      901 tagtttctct aattatttta gccacatcca tgcctgttag ttgaagcatt ttaatatcta
      961 aaaaaaatat atcaatacct tctaaatttg tttccaataa ttcttctcct gaattaaact
     1021 caaatagttc aatttgacta gaaataccct ctaaggactt taatatacac tctcttaaaa
     1081 aagctatttg tgtaatatca tcctcacaga taactattct atacataaac acccctcctt
     1141 agacattaga tctgaaaaca aataaaatat caatttataa ataattattt agttatacaa
     1201 ctaacatggt catcaaatac tattattcac ttaaaaaatc accccctatt tttagtatgt
     1261 taacaacaaa ataagaagat atcttagatt tttaaaaaca attaatactt aatgtagttt
     1321 tgtatactat ttataggtaa ttttttatat cattttaaac attaaaatta ttgatgttta
     1381 agaactatta tattgatagt aatatataat taatgatatc aaaaattttt aattagactt
     1441 ctctcttatt caagattttt ctatataaaa tctaagaata aatagaaaac taacatctaa
     1501 ccttttattt tttattttcg atctatttac ttgaatttta acataaatct atcttttagt
     1561 atatattaat catgctgaat ttgtgatgac atgtatacaa aatatcgttt agtattttct
     1621 aataaaaaat attactgaaa ttaaaaatct acaatagcat ttaacaagta aaaacatgtt
     1681 tacacaaata tataatttaa ggtctttaaa atagagtatg tattattcta aaaattagtt
     1741 atagttatca cataaaaata gaaaagaatt ttcaaaagat ttaaagaaca tacatacttc
     1801 aattgcagaa gatgtaatgt taagtgaact agcattcttc gaggaaaaat agactcaaaa
     1861 aattagaaaa tagaatcagg ttttagatga tttaagtatt tattttcaaa aaagaaattt
     1921 gattctaagt taataaagaa atatttaaaa aaatcaaaaa attttcttaa ataagtaata
     1981 ggttttgtaa aaatggaaaa atatcaaaat aagactatga tttttaaaac tatagcctta
     2041 tcaataaatt tttatttaaa gaacacacat aaatattttt aaacctaatt tatgtgaagt
     2101 tttatgtatt ttttatataa gtagtattct aatcttactc atctaatata ccaaaaagac
     2161 tgtaagtaag ttcactttta tgcaaactta cccacaatct cttttacaca ttaattctat
     2221 ttattaaaat taaaaatatt tactgctatt ctacccaata aagatgatac tgttgttgtt
     2281 ctggctggta ctgttacttc tatttctcct gtatctgctg ttttaccact tgatgtgtgt
     2341 gtcactgtaa atactaatgt tacttttgtt tctgtatctg gtggcactat tgt
//
LOCUS       DAEOSP010000304         2379 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.308, whole genome shotgun sequence.
ACCESSION   DAEOSP010000304 DAEOSP010000000
VERSION     DAEOSP010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.308"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            138..521
                     /locus_tag="KRH54_003481"
     CDS             138..521
                     /locus_tag="KRH54_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBG8581110.1"
                     /translation="MNLTKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            523..873
                     /locus_tag="KRH54_003482"
     CDS             523..873
                     /locus_tag="KRH54_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581111.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(974..1432)
                     /locus_tag="KRH54_003483"
     CDS             complement(974..1432)
                     /locus_tag="KRH54_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581112.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(1477..2265)
                     /locus_tag="KRH54_003484"
     CDS             complement(1477..2265)
                     /locus_tag="KRH54_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG8581113.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
BASE COUNT          855 a          357 c          292 g          875 t
ORIGIN      
        1 aaaatttaaa ttgtaatgaa taggaagtaa ttaaaaagaa tttttattga tttttaaaaa
       61 tttagttaga aactatatca ttatggtaaa aaatgtatta taatgtttag aaatattata
      121 ataaggagcg attaaacatg aatctaacga agaaaataga agataaaaga aaagctaaac
      181 agagagctga aaaagttaag aaagctaaaa ttgctacagc tggtgtggta ttaggagctg
      241 ttactggtgc agtgagtgga gtacttttgg ctcctaaatc tggaaaagaa acaagagagg
      301 atataaaaga tgcttcacaa caaattgctg aaaagataaa tatgaaaact gtagatgtaa
      361 aaggcaaagt atctgagaag ctagaggata aaaaaggaaa ctttatagaa tcaaagaaaa
      421 aaataaaaaa atatatagat aacaaaaaga gtgttgaaaa ttctagtgag gaagataaaa
      481 taatagatat agttgaacca gttagtgtta ctgaggaata atatggacaa tacaataacg
      541 attaacataa gtataatata tgtattatta gctatacttg cttgtattgc tttagtttat
      601 ttaattattg tacttgttaa actacataag attttaaata atgttggaac tatgttaaat
      661 gaaaatactc aaaatataaa tacaacttta aataatcttc ctaatgttgt agaaactttt
      721 ggagaagtag gagaaaatat gaaagatgta acagaagtag ttactgaagt tacagcagat
      781 tttcttgtta ctaaagacag tgtcaaatca aatatggatg ttatagttga tatattgact
      841 ataattaaaa acatttttct agataaaaaa taataagtta atataaaata agcctataag
      901 atataattta aacgacctta aaatagcatt tataaggtcg ttttttatta gcttgatttg
      961 acactatgtg tttttaataa ttggtaatat tcctctttat catttgaaca tatatatcta
     1021 tgcttgttgt ttcaattaca tccataagaa catctttaca ttcaagctca aaatcttcca
     1081 ttccttctaa atattcatca tcttcatcaa aaaagatata agaattatat agccattctt
     1141 caaatttttc ttcaagtata tcccaatacg aactccaaaa taatccacaa cctgatatat
     1201 aagatgctct actttgattt atataaacct catttagaag cttttgtaat ctactgctat
     1261 aaagaaaatc tgctcttcca ctgcttgata ttaattcatt taaatcttct attgacatta
     1321 attcaacttt accactttta tcataaggag tataattttc atcaacccat ttatgtggtt
     1381 tgttttctct gcatactgtt gctatcaact ctacaacttt tgttcttatc atttttttat
     1441 cctaacttat ttatttttta taaaataatc atgattttat aatttatcta tttcttgtaa
     1501 tgctaattta acagtttcat atctatcagc accatttatt actacatcac cttttacaag
     1561 tttattagct tctccaccta caacataaac ctttttacct aaaccttgct tataactaga
     1621 cgcctcaatt attaaagaat ctttcaattg ccaatgtaaa atctgagcag ccactttatc
     1681 agcatcacct gtataaacga ttacattttc atacacttta ttttcccctc ccccttgaga
     1741 atttgatata gatttgttta gtataccttc tgctatcaat ttagcaactt tatctttatt
     1801 attaacataa taatctgtat ctgctttact atctacaaaa catacttcta ttaatattgc
     1861 tggtgcaata gtatgattaa gccaataaag acctctaaca tcagactttg cacccctatc
     1921 tttgaatact gtagctaacc tagtattcac tctttctgca taggttttgc cattattagt
     1981 cttatatatt gtttctgtcc ctactggagt tgaagttgta gcatttgaat taaaatgaat
     2041 ctgaacagcc aaatcagttt cctgtttatt tgctatagca cattggtctg ctaaatgatt
     2101 acttgattca tcaactcttc ctgtataaac agtagctcca cctattttaa gccatttaac
     2161 aattaagtca ttcagtattc ttgtttcttt gctttcgttt atatacccaa cagctcctgt
     2221 accttttcca attaaagtgt gccctggtat tattactact ttcaaactaa catctccttt
     2281 acaattaaat ttaacttcta tttaatatat gcataaccta gaattttgtt tctttttata
     2341 tcttatactt ttattataac ctattttgta aaaatatag
//
LOCUS       DAEOSP010000305         2361 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.309, whole genome shotgun sequence.
ACCESSION   DAEOSP010000305 DAEOSP010000000
VERSION     DAEOSP010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.309"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            117..305
                     /locus_tag="KRH54_003485"
     CDS             117..305
                     /locus_tag="KRH54_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBG8581114.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(465..617)
                     /locus_tag="KRH54_003486"
     CDS             complement(465..617)
                     /locus_tag="KRH54_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBG8581115.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(715..1191)
                     /locus_tag="KRH54_003487"
     CDS             complement(715..1191)
                     /locus_tag="KRH54_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBG8581116.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1410..2171
                     /locus_tag="KRH54_003488"
     CDS             1410..2171
                     /locus_tag="KRH54_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBG8581117.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLCYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
BASE COUNT          877 a          324 c          311 g          849 t
ORIGIN      
        1 attcttcaat atgtgtaagg caaatatata tttctattgc aaatttttgt tatttatgat
       61 aatataaata tgtttttagt ctttaaatag ttatcatttt aaggaggtgt gcgtagatgg
      121 caaaagtatg tagcgtatgt ggtaaaggta aggtttctgg aaaccaagta tcacactcaa
      181 ataaacataa taaaagaaca tggtcagcta acttaagaag cgtaagagcg attatagatg
      241 gagctcctaa gagagtaaaa gtttgtacta gatgtttacg ttctggtaaa attgaaagag
      301 cttagtaacc acgtttaaaa gcctacttcc gtaagtaggc tttttaattt aatcattttt
      361 actctttctc ataaatttta ggattatact tgaaatccat tttggtaatt ttattgttac
      421 tactttcatc aatactcacc ccacacttta tagttaaaac tattttaaca aaaaaataat
      481 aatgctccac atccaataag tattaatgcg gtaagtgctg accaaatcca gccaggaagc
      541 aacattgaca gaattacagc cattcctgtt gctagacaaa gtattcctag caatacctta
      601 gacttgttat ttctcatgga acacctccac atagaacctg tatctataaa tattttcata
      661 ttcaagttta tgcgacagtt ttccatttta gaccaataaa cacattatgt aattttaatt
      721 tttcatacta gaattattac tgaactccga tatagattct acaatatcca aaattccttt
      781 ttttctactt aacctttcag gtccactata taaaacaact ggtataactt taggaagttt
      841 aaaattttta tcttctattt cttcaaggct ttttttcata ataaaacttt tccaaatttg
      901 ttctctatac gatttcaatc ttgggacaat ttcagtatcc atttctgttt gaaattctaa
      961 cactatataa tgtgatactt tgccgctttt agaaaatacc ttataaatta tatcaacttc
     1021 ttttccttta tattcacata taaaagatgt ttctaactct atatttttaa gagttaattc
     1081 ttttgctatt gagacacttg taaaattttg aatcaaactt aaaaaacttt ctttatctga
     1141 atacattctt ctgtactctt ctcgtttttc attgcttctt tttaaattca tacagttcct
     1201 cctagactat agaccttcta ttttttagta aagatagtct ataaatttta ttttttaatt
     1261 ttcactagtg agtattgaca gttgtgtgta aaatatccag taaagatatt tttttatatt
     1321 tttattaaaa gatataaatt tttatatttt aatgtattga catttacgtt gcgtaaaagt
     1381 gtatatttaa tttataggag gtactactta tggaatatac agttcaaaaa ttaagtaaac
     1441 tagctggtat aagtactaga acacttagat attatgatga aataggtctt cttaagccac
     1501 ttaaaataaa ttcttcaggt tatcgtatat atggtcaaaa tgaagttaat aaacttcaac
     1561 aaatactttt ttatagagaa ttaggaatta acttagaaaa tataaaaaac attataaatt
     1621 caccaaactt cgatagttta agtgcgttaa aagaacacca taataaatta ctagccaaaa
     1681 gaaagcaaat tgatttacta atagaaaatg taactaaaac tatagctctg aaggagggaa
     1741 aatacactat gacagatata gaaaaatttg aaggttttaa agaaaagatg attgatgaaa
     1801 atgaaaaaaa ttatggaact gaaattagag agaaatatgg taaagatatt atagatacat
     1861 caaataaaaa acttaaaaat atgtctaagc aagattatga aaattggcaa aacctaaata
     1921 tcgaaataat tagtaaactt aaaaaagctt ttaaaactgg tgatgcctct agtgaactgg
     1981 ctcaggaagt tgcaaaatta catcataaat ggttgtgtta cacttggaac acatactcta
     2041 aagaagctca ttcctctcta gcacagatgt atgtatatga tgaacgtttt acatcatatt
     2101 atgatagaga acaacctgga ttagctaagt ttttaaggga tgctatagta atatatacaa
     2161 gagataaata atataaaata ataaaggtgg tgtctcaaaa tgaacttttt agttcatgag
     2221 acactctttt ttgtatgaaa tcagatatat ttttatcatt ttatcaatga aaaacaggtt
     2281 tctctctcta ttttgagaca gaatctttat tgtataataa ttataatcaa tacaaaataa
     2341 taacaaatta tacaaaactt a
//
LOCUS       DAEOSP010000306         2356 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.310, whole genome shotgun sequence.
ACCESSION   DAEOSP010000306 DAEOSP010000000
VERSION     DAEOSP010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.310"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            62..487
                     /locus_tag="KRH54_003489"
     CDS             62..487
                     /locus_tag="KRH54_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8581118.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIC
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVRKEE"
     gene            519..1706
                     /locus_tag="KRH54_003490"
     CDS             519..1706
                     /locus_tag="KRH54_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBG8581119.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCISAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            1898..2302
                     /locus_tag="KRH54_003491"
     CDS             1898..2302
                     /locus_tag="KRH54_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBG8581120.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLKYKPDK"
BASE COUNT          957 a          224 c          413 g          762 t
ORIGIN      
        1 gattagttga tagaatttaa ataataaatc caatattata aacataggag gtatgaaaaa
       61 tatgattaat gagaatttaa aaagtctaag aaaaatcaac cagtatacac aagaagaatt
      121 agctgaaaaa cttaatgttt ctagacaatc aatagcaaaa tgggaaagtg gagaatctat
      181 tcctgatata tgtagttgca ttaaacttgc aaaattgtac aatgtcaaac tagatgactt
      241 agttaatcat tcagaagaaa aaacaggtat tatagtacct cctaaaggaa agtttttctt
      301 tggagcagtt gtagttggtg aacgtggtca gattgtcatt ccaaaagaag caagagaagt
      361 atttaatata aatgcaggag ataaattatt ggtacttgga gatgaggaaa gaggattagg
      421 aattgttcat caacgtgatt tgataaattt tataggtcat gtgggagtag tacgtaaaga
      481 agaatgattt aaaaaaatta taaaaggagg tatgaaatat gagtaaaata gtatttttta
      541 gcattcctgc atatggtcat actaatccta ctattgaagt agttcgtgaa ttagtggata
      601 gaggaaatga ggtattgtat tattctttta atgaatttaa agataaaatt gaaggtgctg
      661 gagcaaaatt tatttgttgt gataaatacc ttccagaatt actccctgga gatgagaaaa
      721 aaattggtaa ggattttcct ggattaattg agatgatagt agatactact atttctttag
      781 atgaaaaagt ttgtagagaa cttaaagatt ttaatccaga ttgcattgta tcagattcat
      841 taagtttttg gggaaagtta tttgcacaaa aacttaatat tccatatgta tgctctacaa
      901 caacttttgc atttaataaa catacagcaa agttgatgaa acaaaacttt atagaaatta
      961 tacgtatgat ttttggtgta agacgcatta ataaaaaaat aaagctttta caaagaaaag
     1021 ggtatgaagt aaaaaatttt atttctatag tttctaatga taatgatacg gataccattg
     1081 tatatacatc aaaagaattt caaccaatgg tagaaacctt ctctagtaag tattcatttg
     1141 taggtccatc agtatcaaag ttgattattg aacctaaaga gagaaaaaga aagctaatat
     1201 atatttcgct tggtacaatt aataataaaa atgttagctt ttataaaaac tgtataagtg
     1261 catttaaaga ttctgatgtt gatgtgatta tgtctgttgg tagaagtact gatattaaaa
     1321 gtttaggaaa tataccaaat aattttgagg tcaaaaatag tgttgaacag attgctatac
     1381 ttcaaaaaac tgatgtgttt gttactcatt ctgggatgaa tagtgttaat gaaagtttat
     1441 attatggagt acctatggta ttatttcctc aacattcgga gcagagaatg gtagctaaga
     1501 gagttgttca tttgggagca ggaattatgt taaaagagga taagtcagaa agtattaaaa
     1561 aagcagtatt tcaagttata aatgacaatg aatataaaga aaatgcaact aaactatcaa
     1621 aaagctttta taatgcagga ggctcaaaaa aagcagcaga tgtaatatta cagattatat
     1681 gtaatagtag acaagttgac atataataga tttgttaaaa atatatatac atacatgtaa
     1741 tttaatcaaa gtactgacag tggggtcttt taacctacca gttggggcat atatattgat
     1801 gctaaatatg agggatgtta tattttaata aaaaagataa catccttttt ttagtgcatt
     1861 atatcgagtt ttaaataata aatgaaggag gatcaatatg aaaaaatata aagttatttt
     1921 tatagtatta attataggta ttataagtgt aacagggttt gttgtatata aaaactgcat
     1981 taaaccatgg aaagatgctg aaacatatat tgataagtat atggtaaaac aagatatttc
     2041 aaaagataat ataaaaagta ttacaaaaga aaaagccaag aaggcaagtt acgaaggaat
     2101 actatacaaa gtgtattaca aagatgaccc tcgatatgag tatcaatatt tttatagtga
     2161 taattattat gctctatatg taaataaagt aatgttacaa atttatgata tggaaaataa
     2221 taataaactt ttaagaagta atgaggaatt aaaaagtgta aagtatccac caatatatct
     2281 taaatataag ccagataaat gatttatgac aaaatagaat gatttttatt aaaatttatt
     2341 taaaaatatc actaga
//
LOCUS       DAEOSP010000307         2346 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.311, whole genome shotgun sequence.
ACCESSION   DAEOSP010000307 DAEOSP010000000
VERSION     DAEOSP010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.311"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            66..1691
                     /locus_tag="KRH54_003492"
     CDS             66..1691
                     /locus_tag="KRH54_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBG8581121.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            1890..>2346
                     /locus_tag="KRH54_003493"
     CDS             1890..>2346
                     /locus_tag="KRH54_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581122.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDI"
BASE COUNT          938 a          239 c          460 g          709 t
ORIGIN      
        1 ataaaaatgt tatcaaagtt ttatgataaa ataaattatg agcaaaaaga aaggaaaata
       61 tattaatgtt acaagttaca ggtgttggac ttagatttgg tgataaggaa ctatttaaag
      121 atgttaactt aaaattcact aaaggtaatt gctatggaat aataggagct aatggtgcgg
      181 gaaaatctac ttttttaaaa atattatcag gagaaataga gcctaataca ggtagtatat
      241 ctataactga taaagaaaga atgtcagttt taaaacagga ccactttgaa tatgaagaag
      301 aaactgtttt aaatgttgtt ataagaggtc atgagagact ttggaacata atgaaagaaa
      361 aagatgcatt gtatatgaag gaagacttta atgaagaaga tgggataaaa gctgccgaac
      421 ttgaaggtga gtttgcagag cttgacggat gggatgcaga aactaatgct gagaaaatac
      481 ttatgggcct tggcataacg aaagatatgc attacaaaca aatgaaggaa ctagttggtg
      541 gggaaaaggt taaggtattg ttagcacaat cactttttgg taagcctgaa atactattaa
      601 tggacgaacc tacaaaccac ttagatttta aatcaataaa ctggttaaat aattttataa
      661 tggatttaga agaatctata gttataatag tatcacatga tagacatttc ctaaatcaaa
      721 tatgtactaa tatagttgac gtagattttg gtaaaataca aatgtacgtt ggaaactatg
      781 atttctggta tgagtcaagt caattggctt tacaattggc taaggaccaa aataaaaaga
      841 ctgaagaaaa gatagctcaa ttaaaggaat ttatagctag atttagttct aatgcttcaa
      901 aggctaaaca agcaacatca agaaagaaac agttagagaa attagaagtt gaagatatac
      961 aaccatcaag aagaagatat ccatatgtag gatttacacc tgcaagagaa attgggaatg
     1021 aagttttaga agtacacaat ttaactaaaa ctatagatgg ggttaaggtt cttgataatg
     1081 tgtcatttag attggataga gatgataaag tagtatttat gggagatgaa atagctacta
     1141 ctgcactttt caacatagtt atgggagaac ttgaaccaga cagtggagag tataaatggg
     1201 gagtaactac ttctcaagat tatcttccaa aaaatcataa taagtttttt gacggagtag
     1261 aatattcttt ggttgactgg cttagacagt tctctgaaga aaaaagtgaa agttttataa
     1321 gaggattttt aggtagaatg ttattctcag gtgaagaagc attaaaagaa gctcaagtta
     1381 tatctggagg agaaaaggtt agatgtatgc ttagtaagct aatgctttca aatgcaaatg
     1441 tacttgtatt agatgaccct actaaccact tagacctaga aagtataact tctgtaaata
     1501 aaggacttga aaagttccca ggagtgcttt tatttacttc tcatgaccat gaatttatat
     1561 ctactatagc aaatagaatt atagagataa ctccaaatgg aataatggat agaaaaatgg
     1621 actttgatga gtatttagaa agtaaagaga tacaagacca attagctaaa atgtatggaa
     1681 aagataaata attattagta taaaaccttt taatatttga gacatgctat gcatgagtgt
     1741 gtaaattttt aatcaaatat taggaggttt tttatgttgt ttaaacatgt ttattagcag
     1801 aatagagtaa tattttgtat attaagaatt taaatataat atagtagatg atataataaa
     1861 aatatataac attttggggg gaatagatta tggttaagaa aaggttagta gctgtagtaa
     1921 ttagtttagc tatgattagt gtagggaatt tttcatatgc tcactcagga gggcataatt
     1981 catcaggagg tcatcataat aaaaataaat cagagttaac acgttattat tgtggaggac
     2041 attcagcaca tcaacacaat aaaggttttt gtccttatgc tagtacaaaa gaaaattctg
     2101 gagtaacaat atcttcagta cataacgaaa aaaatcataa aaactatttt gcagaaaaag
     2161 ggtataataa aggttatgaa gatgggtgta aagggcttta taatttaagt gtcacagaag
     2221 ccaaatctga taatttatat gaaaaagaga ttttaaaaag ttcatatgaa aaaggatatt
     2281 taattggata caatgaatat aaagcaaaaa aagtagatga tgtaatgaag aagatgttag
     2341 atattc
//
LOCUS       DAEOSP010000308         2333 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.312, whole genome shotgun sequence.
ACCESSION   DAEOSP010000308 DAEOSP010000000
VERSION     DAEOSP010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.312"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            225..1526
                     /locus_tag="KRH54_003494"
     CDS             225..1526
                     /locus_tag="KRH54_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBG8581123.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1602..1877
                     /locus_tag="KRH54_003495"
     CDS             1602..1877
                     /locus_tag="KRH54_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBG8581124.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
BASE COUNT          959 a          189 c          459 g          726 t
ORIGIN      
        1 ttaataattg gcagtaagtt atatcaaata agatattgct attatgtagt aaattatata
       61 aaacaatgta tcagaataaa tagtatatta ataatttgta gtatatcatg acgaataata
      121 cagtcataac aaatagtgag tcataacaaa taatgtattt ataattgata atgtatttaa
      181 aaatcaaaat ataaaaactt ataaggaaag agagattaat ttatatgggt aaatcaaaag
      241 atgttgaaat agagaagtct attttaggca caattctttt agacaataaa ttaagctata
      301 agctagatga attaaatgaa aacatgttta tgaatgatat atgtcttgag atatttaaaa
      361 ttatgaaaga acttaaaaaa gaaaacatag tgattgatgt ggccactgtg aaatcaaaga
      421 tagatagaaa atctctagca ataaaaacga gtgatgtgac taatctaatt acttggggtc
      481 agaattttgg tcttgatggt catattaaaa ttttaaaaga aaatttagct agaagaagca
      541 taaatcaaaa ctgccaaaac cttttacaca gcctaaacct tggagaaaat atcgatactt
      601 gtatttacaa atttgaaagt aatataaagg agattttgga caaggatact tatgaaaatg
      661 atgatgtaaa tagtatagca gggaaggtgc ttgatttttt agaaaacaaa aaagatattg
      721 gatttaaatt tggtataaaa ttacttgata ccactatagg aggtcttttt aaaggagagc
      781 taaccacaat agcagctaaa tcgggggttg ggaagactgc tttggcactt caaataatgt
      841 taaattcatt taagcaaggt aaaaaaactt tatttataag tagagaaatg accagtgagc
      901 aagtatttat gagaaatata tgcagagtaa ctggtgtaag tacaaggaat atgaaatcta
      961 aagaaataga tgaaaatgat tggaaactta ttgtaaatgc tataggtgat ttaagcgaaa
     1021 ataacttaat ttatatcaat gataagatag atacaattag tgcaattaga aagaggataa
     1081 ggcaggtaaa gccagattta ttaatagtgg attatgtgca actccttaca tctcagaaaa
     1141 gcatggataa gagagaaagg gaagtagcta ctttttcgag agaacttaaa aatatgactt
     1201 tagattttaa tatacctgta atccaattaa gtcagttaaa tgatgaaatg aaagattcaa
     1261 gaccttatgg agatagacct atgagggata gcaaagccat ctaccacgat agcaacaatg
     1321 tggtttatat acatcaatta aaggggagtg attatgaaga agcagtgaga gatattggag
     1381 aaagtgagga ggcagttaga gcatcagaat acaggggaat taagatggtt gatttaatag
     1441 ttgcgaagtg tagagatgga cagactaggc ataagcattt ttgttacttt ggggataagt
     1501 tacattttca agaactaaat tattaggtca ataaagtagt aataacaagc ttattaaaag
     1561 tcgggaaata gcaaatagat tataaaatat gaggtgaaaa tatgttggaa ttaagaaaaa
     1621 ttatagaatt acttgaagaa cacaagatag aagtaagtat tccaaaggag ttacataagt
     1681 atagtttatt ggaatacctt gaattgtgtc aacaaagtgg gggaacatat ttttatatta
     1741 aaacactgga aggggcttta aaagaggcta gaaatgagct tatgattaaa ttgtttaatg
     1801 ggaaaaatta tatggaatta gcgaggatgt ttgaaatgag tgttattaat gtcaggcata
     1861 ttgttagaaa aaaatagata tttttggaga atacaagtgt gatatacttc tgaaatagaa
     1921 gttattatag ttgaaataaa attgtggtta ataaattaaa gttgtgtata agtttaagat
     1981 tatagaggag tataagtgtg attaacaaca tgattttgtg aataaaattg aggatatata
     2041 tgagttaaat gtgtgattaa taaggtaagg ttgtgtataa gattatgagt atttaaaagg
     2101 aggggctata attaatagtg tagagttgtg tgtttgctta agattatata ataatttaat
     2161 agattaaact atactaataa ggtaaaatcg tgtgtttagt taaagcttat tataaaatct
     2221 tgatatgtga ttaatgatgc aatatgtgaa taaagttaag attataatag atttaacctg
     2281 tggctaatag aataaaattg tgaataaagt taagattata ataggtttaa cct
//
LOCUS       DAEOSP010000309         2268 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.313, whole genome shotgun sequence.
ACCESSION   DAEOSP010000309 DAEOSP010000000
VERSION     DAEOSP010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.313"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(112..1679)
                     /locus_tag="KRH54_003496"
                     /pseudo
     CDS             complement(112..1679)
                     /locus_tag="KRH54_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(2015..2197)
                     /locus_tag="KRH54_003497"
     CDS             complement(2015..2197)
                     /locus_tag="KRH54_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581125.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSGKV
                     CNTTYKAPIERSEGFF"
BASE COUNT          706 a          310 c          254 g          998 t
ORIGIN      
        1 tattagaaga agataatatt taaataaaaa ataccttata aagttaattt tttaatactt
       61 tataaggtaa atacatattg tattgtgggg ataaatttaa atcatttgat actaatcaac
      121 actaagaacc aataactctc tatcatcttg agtaattgta tatattctta atttcttgta
      181 tgtcataata ttttctccac ctgtaaaata actcctaaga ttaacacaat cagttttagg
      241 ttgatttgta tctcctaaat aattacgcaa ttctttatct ttagtttcaa tatctttaaa
      301 tccaaaatat tgtaaagtag cttcaactct atatttttgg acataatcga gagcttcttt
      361 attagtttat gttggtgtaa aatacatacc atttatataa gcctttattg tacttggatt
      421 aaatttaaaa tctgcagaat atattttatg tgtaactatt atttcttgaa cagttggaat
      481 tttaacttct ggttcatcaa gtatttcagg agtactattt agctctgtaa tagataagtt
      541 atctaagtat attgtatcac tatcaattaa tgctatattt atattagaag aatctttttc
      601 agtagtttca aattcataac taaattttgt atatccttgt tctagtacca aataatccgt
      661 tttttcttct tttactttta tatttacaat tattgaatta tatgttaaag gattttttga
      721 attttactaa aaacataacg tttataaggt tttagttcag acataggtat tttaatcttc
      781 gtctcaccat ttaatctatt tgctgagcct tttaaaccat cttgatttgt agtattaaca
      841 ttactccata tactagggta attattgtaa tcatcaaaat tagtaaagta agttggtgtt
      901 tttataagta tattcattcc tctttcaagt ttaacattac atatttgttt atcagacaaa
      961 gtttttaaac tgtctttaat cttattgcct atattatcat caaatatgag ttcaacacaa
     1021 ctttcatcta ttggtatatt attaaaatat aacaaaccat cttttttagt agtgccaaaa
     1081 gttttttcaa ttgcttctcc aattgtaagt tcaggtgttt tatcttctgg gacctgtaaa
     1141 cttttagcag taattcttct tttgtaagat tcattttctg tatctaatat aatagataca
     1201 gaaatactgt caatttgacc tatatcgtct taccaactat ttcattctgt tactatttgt
     1261 ccagaaccat tttttgtacc aaaatttcca cttacttgtg ttgtttctaa tttaatttgc
     1321 tttccatcat ctaatttttt taattaatta taatttatag gaatcagtct agaactaaat
     1381 tgatacattg tgttaagagc cataggtgaa aatccttttt tgagataagt atttccagga
     1441 gatagattat tgccaatttg attttcttgt gctttgatag ttgataatgc atctccatct
     1501 aacactaaat ttgttgttgg tgtcactttg tacataggtg cagtacctgt gttgtagtat
     1561 ctaatatttg catttatata tgcagattct cctttgttta tacttaatcc agtatttcat
     1621 gattatccat tgctattttg aacagcagtt gaattatctg ttgtatggta ataatttgtc
     1681 ttgtatagtt tctttaaact cattaaattt ttctaatgaa atttcgtttt gattttttgt
     1741 tcttattact ttattaaatg caaatttttc tggagattca aaataatata catacatagg
     1801 tttatctatt ttattttttg atatagcatt tcgaagttct ctatattctt tttctcaaga
     1861 atttgcttct atttgataat cataaaaata atttcttgtc tgagaatatt tgcttatctc
     1921 tatagaatct tttttatatg atcctaatac ttctttttca gatttttcaa gtttttgatc
     1981 ttttctttct cattctttag ccttttcttt atctttaaaa aaatccttct gatctttcta
     2041 taggagcctt gtaagtagta ttacaaactt ttccactata gctttgtaaa tcttgtgcat
     2101 aaatgttatt aggtaacaaa ctacctaaag ttagatataa tatcaacaat atagatatac
     2161 aattactaat acttttatgt ttcctaaatt ttttcattta ttctccctac aaatattagt
     2221 ttagttaact tttgaatttt aatttattta gattatataa tctaaata
//
LOCUS       DAEOSP010000310         2247 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.314, whole genome shotgun sequence.
ACCESSION   DAEOSP010000310 DAEOSP010000000
VERSION     DAEOSP010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.314"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            99..416
                     /gene="trxA"
                     /locus_tag="KRH54_003498"
     CDS             99..416
                     /gene="trxA"
                     /locus_tag="KRH54_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBG8581126.1"
                     /translation="MVKIVNNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            complement(623..1897)
                     /locus_tag="KRH54_003499"
     CDS             complement(623..1897)
                     /locus_tag="KRH54_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBG8581127.1"
                     /translation="MQFMDVSKYIEGANLKVKTKSGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPLLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
BASE COUNT          789 a          301 c          325 g          832 t
ORIGIN      
        1 tctattaaaa ataggcggtt acaaaaaagt gcctaattga actgttatat aggtcgtagt
       61 atattaatat caagaagata ataaaggaga tgtatattat ggtaaaaata gtaaataata
      121 atgaatttat aaatgaagta gaaaataaag atggtttagt agttgtagat ttctttgcga
      181 cttggtgtgg accttgtaag atgctatcac caatatatga agctttagga aatgagatgg
      241 ttgaaaaagc aaacttcttg aaggtggaca tagaccaaag tatagaatta gctcagaaat
      301 ttgaagtatc tacagtacca actatgctaa tatttaagga tggaaaacct gtagatagat
      361 taataggttt tatgcctaag gagaatttaa aaaacaaaat tgaatcatat atgtaattaa
      421 agtaaattga agtaattaaa ataaatctca tatataaaca aaaaaactca ttgtaatgag
      481 ttttttgttt gtatagtgta aaatagtttg tatagttaca aatgagtgtc taattgctaa
      541 tgaaattgac tgttgatata atttgctttt ttgtgaaaac aaaactactc tctatttatt
      601 gataaaaaag aatagaaaaa acttatttaa aagccaaagt tttaatagca tgtataaagc
      661 tatccacctc atcaaaagta ttgtacattc ctaagcttat cctaacaaga cctacaggat
      721 tttccctcat aaatctgaca tatgcttctt ttggacttat acctaataat ttttgtacat
      781 aaatatctgc acctactact ccagctccag tttctattcc aaagttatca actaaatatt
      841 ttgctacatc tttgtaatac atattactta tattaaaaga tatatttggg ataattacat
      901 tatttgattt atctccatat attattatat taggtatttt actcatttct tctttagcat
      961 ataaaaataa ttctctttcg tattttgaaa ttctgtttag accaatttta tttaaagtgt
     1021 tcagtgcttt tcccattgca agagttccaa aatactctgg gtatcctgct tcaaaacgtt
     1081 ggggtacgtt tgcatatagt attttgttat catttacaaa tttggtagaa cctgagccat
     1141 atagtacagg attaaatttt tcaaagaatt ttttaggtcc taccagagct cctccattta
     1201 gtggagaata acatttatga gcactaaaaa caacaaaatc tatatgctca tcacattcat
     1261 gtggcatcat tgaaaaaggt ttatgctgaa ttacttgcac aatatccacc aatatctttg
     1321 caccatactt atgagctatt cttgctattt catatatagg agtggttata cctgtgacat
     1381 tactagctcc tgtaactgta acgagagata cattaccagc ataattttta agtatattct
     1441 ctaattgaca taaatcaagt tctccttttt ctgtaattcc aacaactttt gtaggaaaat
     1501 taactttaaa aggcaggtaa ttagccatat gctccattgc tgtggtaata acaaatttat
     1561 ctggattttc atctttaaaa aattttgcta agaagtttat tccttctgta gcatttttga
     1621 cataaataac agaatccttt tcttcgtctc catcaataag tttgaggata gtagagcgta
     1681 cattttcata ttttaaagta tttttttctg agattattcc tggagcatta atataagtaa
     1741 gcaaaggaat ctcacagttt aagttatcca ctacgttttt taaaactaat ggagtggcag
     1801 agttgttaaa atatattctt ttaacagttt caccactttt agttttaact ttaaggtttg
     1861 caccttcaat gtatttagat acatccatga attgcataaa aaaacccctt atctaataaa
     1921 ctattctgtt ttttattaaa aaagctcaag ttttaatcaa gagcttgttt aatatatgtt
     1981 atgtttttat aaggggtttt gttaattaat tcttgttgag atgtttatta aattacacga
     2041 aaatgagcta cagttggggg aaactgtagc tcacttgatt ttgggggaga taggttgaac
     2101 ttattgttta tccgtatcat atttttagta tagtcactta ttttaattta tatacatttt
     2161 tttataaatt ttacataaaa tgttaaacac taattagaaa taattatgaa tatgactaat
     2221 aaagttgtaa aaatagtaaa gaaattg
//
LOCUS       DAEOSP010000311         2242 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.315, whole genome shotgun sequence.
ACCESSION   DAEOSP010000311 DAEOSP010000000
VERSION     DAEOSP010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.315"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            216..>2242
                     /locus_tag="KRH54_003500"
     CDS             216..>2242
                     /locus_tag="KRH54_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBG8581128.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKE"
BASE COUNT          813 a          253 c          434 g          742 t
ORIGIN      
        1 ttttaaaaca ttaagattgt tactgataat gcaggcaaga cttatgttta taaaaaaatt
       61 ataatagttt atttttaatt ctgaaattag gttaatatag gtttatttat attaacataa
      121 gtttaatttt agaactaagt atactaatgt aactttttat aaacttaagt cttttttatt
      181 taaaaaaata taaaataata aggagagaaa agtaaatgaa aaaagtattt ggtgttttac
      241 aaaaagtagg aaaatctcta atgctaccag ttgcattact tcctgcagca ggtatattac
      301 ttggcgtgag taatgcttta gcaggagacg cattgttggc tcattttcca gctttggcga
      361 atcctacatt tcaacttata ataagtataa tggaaagttc aggtcaaatt atatttaata
      421 acttaccact tatatttgca gttggggtag ctgttggttt aactgaagga gaaggtgttg
      481 cagcattggc agccatagta ggttttttaa ttctcaatac atctatgggt gttatggcag
      541 gtgttacaga agatatggta gacaattcta tgtatgcaaa tattgttgga ataccaacaa
      601 ttcaaacagg ggtatttggt ggtattatta tagggatagt ggctgcttat ctttataaaa
      661 agttttataa aattgaatta cctcagtatc taggtttttt tgcaggtaaa agatttgtac
      721 caatcgttac ttcattagtt gctatagcaa taggaattat attgtctttt atttggcctc
      781 caatacaaga gggattactt agtttctcaa aaaatgttat agaagcaaat caaacactag
      841 cagcatttat ggaaggttct ttggaaagac tattaatacc atttggtttg catcatgttt
      901 tccataatcc attttggtat caatttggag aatatgtaaa taaagctggt gagcttgtaa
      961 tgggtgacca aaagatattc tttgctcaac ttaaagatgg agtaaatttc acagcaggta
     1021 cttttatgac aggtgcattt ccatttatga tgtttggtct tccagcagca gctcttgcta
     1081 tggtgcatga agcaaagcca gaaaagaaaa aatttgtagc aggtataatg gcatcagcag
     1141 cattaacctc ttttttaaca ggaataaccg agccaataga gtttgcgttt ttatttgttg
     1201 caccagtatt atttgcaata cattgtttat tttcaggttt atcatacgca attatgcaat
     1261 tattaggagt taaaattgga ttcacatttt caggaggatt aatagatttc ttattatttg
     1321 gtgttcttcc taatagaact ccatggtact tggctatctt ggtaggtatt ggttttgcag
     1381 tattatacta tagtgtattt agatttatga taagaaagtt taatttaaag acaccaggaa
     1441 gagaagatga gtcagaaact aacgctgaag tcattgaaat gtctaagaat gaattagcat
     1501 cagagatttt gattgcttta ggaaataaag acaatttaaa gagcctagat gcgtgtataa
     1561 caagacttag attggttgta aatgatataa ataaagtaga taaagaaaaa ttgaaatcac
     1621 ttggagcagc aggtgttatg gttattggca acaatgttca agctatattt ggaccaaaat
     1681 cggattcaat aaagtctgat ataaaagata taatagaggg aaaaacaaag gttgaaagta
     1741 ctgttactaa aaatgttgat aaaaataaag tgaaaaaaaa tatggaagtt gcaaatatgg
     1801 aaaatgcttt agtagaaaag attgattcat taacaaatgg agagatatta gatatagaag
     1861 aagtgccaga ttctgtattt tcaagtaaac tgatggggga tggatttgca attaaatcta
     1921 gtgatggatt gatttattca ccagttgatg gaactattgg agttatattt ccaactaagc
     1981 atgcaataat aataaaatcc caaaaaagtg gagtagaaat acttatacat ttagggatag
     2041 aaactgtaaa cttagaagga aatggatttg aagtatttgt gaatgtagga gatgaagtaa
     2101 aagcgggaga aaaattagtt aaaatggact tagaatatat tgaaaaaaat ggtctatcaa
     2161 caataagtcc tgtagtattt acgaatttag aacaaaatca gaaattaagc attaaaaaag
     2221 gagtaaccac agcaaaagag ga
//
LOCUS       DAEOSP010000312         2240 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.316, whole genome shotgun sequence.
ACCESSION   DAEOSP010000312 DAEOSP010000000
VERSION     DAEOSP010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.316"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            292..2187
                     /locus_tag="KRH54_003501"
     CDS             292..2187
                     /locus_tag="KRH54_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBG8581129.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT          979 a          234 c          420 g          607 t
ORIGIN      
        1 agagtaaagc aaaaattagt gcatagtagt atataaaaat ggagtttgaa tacttttgtt
       61 agtatatgat tgtttagtag aaaatcacta taaaatagga tttctatctt tgagcataat
      121 tatagagagc ctaagtaggg ggaagggttt caagaagata ttattagtgt ttgtgttagt
      181 aaattatgta aacacataaa atatattgaa aaataatatt atatataata ttatttatgt
      241 gtgggaaata cgaatataaa aaataaaaaa taatattcgg aggacataaa tatgttaagc
      301 aacaaaaaga gaagtatggc aatagtaatg gcaggtgcta cagttatgag tgcagcagca
      361 ccaatattcg cagacaatac cgtgacagaa aatgttgata aaaattacac agtaagtgcg
      421 aaagattctg ctaaactaat cgaagaggtt agaaaagcat tagaagttaa gtttgaagat
      481 acaaaagcag gagcaaatgt aaatgataga gtttacgata taaaagtaga caatgtaaac
      541 ttaactaatg caactcaatt acaaaataaa ataaactctt taacagaagg gcaaagttta
      601 aaagttacta ttcaagataa agggcatcaa gtgttaggtg gaaaagtagt tgactataaa
      661 attgaaaact acaaaactgc tcaagaaata gtagatgcag ttaatgctta caatgcaact
      721 ttagcagaag attctgataa caagttaaca gcaactataa aatctacaaa tacagttgaa
      781 gtaaaaagag caaaagattc agcaaatgtt attacattaa atgtaggaga ccaacattta
      841 gatttctcaa aagtgataac atcagaagaa ggtacttttg aaggttatga aaagagttat
      901 agtgacatag attcaaaaga attacatacg ataacagtta aaaacgcaga tttacaagac
      961 atatcagcag aagaattatt tgatggaatt agattaacta cattaggaag agaaatagtt
     1021 aataaagtta aaaatggata tgctttaaca tttgaaaatg aagcaatcct tactcaagaa
     1081 caagaagata gtgatgataa agataaacca gaaaaatcaa gctttgatat agttttaagc
     1141 aaggcaaatg aaaagccaga aacaataagt gtttcaagta aaaaccataa gttagttaga
     1201 gacttacaca aagtattaac tgatgtaaaa gatggaaaag aattaaaagt agaagtttta
     1261 tctggtgatt caagatttac aacagcagta gaagttagta aagagagatt taaagatgga
     1321 gaagcagaag ctataatttt agttggagaa gatgctatag ttgatggatt agcatcagca
     1381 ccacttgcct ctcaaaaaaa tgcaccaata ttattatcta aaaaagattc actaccatca
     1441 gaaatagaag ctgaaatatt aagagtactt ggaagtaatc tatcttctaa gaaaatatat
     1501 atagtaggtg gagaatctaa agtatcaaaa gaaactgaag aaaaactttc taaattaggt
     1561 gtaagtaaag ttgagagagt ttctggagaa gatagatttg aaacttcttt agaaatagca
     1621 aaacaattaa aagatacatt taagactgct tttgtagtag gtggaaatgg agaagctgat
     1681 gctatgagta tatcagctag agctgctcaa tttggtgctc caataatagt tacaggtaac
     1741 gaattagatg caaatgctga aaaattatta aaaggaaaag aattagaaat agtgggtgga
     1801 gaaaattctg tatcaaaaga agttgaagac aaattagttg atatagattt aaataataaa
     1861 gttgaaagat tagctggaga aaatagaaaa gatactaatg ctaaagtaat caataaatac
     1921 tatgcaggtg caactaaagc atatgtagca aaagatggtt atgtaggtgg aaatggacaa
     1981 ttagttgatg cacttacagc agcaccactt gcagctagtt caaaggctcc aatagtatta
     2041 actacagaag aactttctaa atcacaagaa gaagtagttg agttaagact taagaatgct
     2101 actaagttag tacaaatagg tgaaggaata gctaaaaacg ctattgaaaa aatagcagaa
     2161 aaaataaact tatttactaa gaactaataa gatataggaa ttaatattaa aagtttattt
     2221 agataatata tgattataca
//
LOCUS       DAEOSP010000313         2208 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.317, whole genome shotgun sequence.
ACCESSION   DAEOSP010000313 DAEOSP010000000
VERSION     DAEOSP010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.317"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(75..1367)
                     /gene="brnQ"
                     /locus_tag="KRH54_003502"
     CDS             complement(75..1367)
                     /gene="brnQ"
                     /locus_tag="KRH54_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBG8581130.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGIK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          732 a          368 c          244 g          864 t
ORIGIN      
        1 attcttttaa atataaaaag gaattgccta tataatttaa aggcaattcc ataattatca
       61 attagttttt attattatgc agttttactg cttttagatg gaataaagtt tcctattatt
      121 cctgctatta caactggaac tatccagtta aatcctaatg aagcaaatgg taagctatta
      181 actagaggaa tttttatacc aaaacttgtt gcaactgtta atacacttac taataatgcc
      241 atgtatgcag cacatttgaa cacgttgtca tttttaattt tattactaaa taatgctagt
      301 ataactagag ttatagttgc tggatatacc atacttagta taggagaaga gaattgtatt
      361 attgtactta ctccaaagtt tgatatgatt gcactaaata cacaaactac tataactata
      421 tgctcatact ttaattttcc atctgtaagt gttgagaaat attgaccagc tgctgaagaa
      481 agacctatag ctgttgttaa acatgctaat gcaactataa tagccaataa aattttacct
      541 gtagttccaa gtaaagctgc tgttattgca actattaaag ctgtttgttg aacatcttgt
      601 ccatattgtt gagacacagt tgctccaaga taacataatc caccataaac taatgccatt
      661 cctattactg caattacacc tgcacttatt gtggctttca tcttttcttt ctcacctgta
      721 taacctttag ctattactga catgattata attgatgcaa atacactagc tgccaatgca
      781 tccattgttt gataaccttg agttatacct tctgcaaata cattttgtat taaagaactg
      841 tctcttattt ctcctaatgg agatacgata cctttaacta ttaatactgc taaagctata
      901 agtaaagctg gagttaatat tgacccaact atatctacaa cttttgatgg tcttattgtt
      961 aaaaccaata ctagagcaaa gaatactata gagaatatta ctgggcttat tccagaacct
     1021 attgtaggta atatacccat ttcataagtt gttgctgctg ttctaggtat tgctaaaagt
     1081 ggccctatac agattaccat tgcacatcct aatgcaatac ttaatccttt gcctgctctt
     1141 gagaacatct tcgatgtgtc tccattgaac ttagttgctg ctataactgc taataatgcc
     1201 aaaccaccat ctgcaaaaag gaatcctatg aaagctacta gccaactact tccagataaa
     1261 actcctagat atggagggaa gattaagttc cctgcaccaa aaaacattgc aaatagtgca
     1321 aatccaatta caattacatc tttaaaattg atttgtttct cacccattta aagcaccccc
     1381 atttgaattt tactaccttt tactaaaaaa taagtccaat ctcttaatta aatgaaaacc
     1441 ttttgacgag attttattta attatacatt tttgacttaa atttgtcaat tatgtttcat
     1501 aatttataaa atcttcacaa tatttaatac aactttctta attaaacaaa atcaacctgt
     1561 atactttaaa catagttggt tttttgtttt ttctctaatt tttcttgata aaattaacta
     1621 ttccgaataa ttaaaatatt aagatttttt agattattcg atttattatt aagttgtatt
     1681 gctttatata ctgtatatat cactctcctt ttttacattc atataatctt tttaattcaa
     1741 tttaaatttt aaatattttg tgtttattat ataatctgaa aaaaaagcag tcaatagcat
     1801 ttttaatttt tttaattttc aaaattatga ttctcaaaaa atcgttttcc ttttatcata
     1861 acacctattt acgttaattc ttgtagtatt ttgtatttat tttgctaaat tgacagttaa
     1921 ctttattcaa tttttattct acttgcgtag atttacctct tgttttttac tcttaaaacc
     1981 attttttctg aaaattcctt attatgattt atcaacttat ttttcttcat aacaattact
     2041 ctctaacttc caacttactt aaactttgca gttgctagta ttcacgttta catacttata
     2101 tgtcataatt aactaaaaat tcttaatata tctctcaatg aaaacaattt actcatttat
     2161 ttaatacatt tatataatca tatgtcttaa attttgtttt attatttt
//
LOCUS       DAEOSP010000314         2025 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.318, whole genome shotgun sequence.
ACCESSION   DAEOSP010000314 DAEOSP010000000
VERSION     DAEOSP010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.318"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            153..1466
                     /locus_tag="KRH54_003503"
     CDS             153..1466
                     /locus_tag="KRH54_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBG8581131.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            1484..2020
                     /locus_tag="KRH54_003504"
     CDS             1484..2020
                     /locus_tag="KRH54_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBG8581132.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
BASE COUNT          780 a          203 c          399 g          643 t
ORIGIN      
        1 aatacaaaag tataattaca ttataattat atgtatttag ttggatttgt gtatttaaat
       61 aatgtataat ttatgctgat tactgctaaa aaagcataat aatattgcat tgtaaggttt
      121 tataaacttc taattgaaag gggataattc aaatggataa gagatattta gaatccgatg
      181 tatttgaagc ttttcaagaa agactttctt atctttttaa tgaatttgat tatatctatg
      241 tatcttttag cggaggaaaa gatagtggtt tactgctaaa tttagttttg gattataaga
      301 gaaagaataa tataagttcc aaaataggag tatttcatca agattttgag gctcaatata
      361 gtttgactac tgaatatgtt gaaaaagtat ttgaagataa tattgaagat attgaaccat
      421 attgggtgtg tttaccaatg gctacaagaa ctgctgtagg aaattatgaa atgtattggt
      481 atccatggga tgatgagaaa gaagagttat gggtaaggga aatgccagtc aaagaataca
      541 taataaatca aaaaaataat ccttttactt attataaata taaaatgaga caagaaagtt
      601 tggcaaaaca atttggtaga tggattagag atatacatga aggtgcaaaa gttgtttgct
      661 tattaggaac acgagcagca gaatctctac aacgttattg ttctattgtg aataaacagc
      721 atggatataa aggaaagtgc tggataacta aggaatttaa aaatgtctgg acggcatctc
      781 ctatatatga ttgggaaata gaagatatat ggttagcaca ctataaattt gactatgact
      841 ataataaaat ttatgactta ttctataaag ctggagtagc tccaagaaaa atgagagtgg
      901 catcaccttt taatgattca gcaaaagaga gtttaaattt atacagaata ttggacccag
      961 atgtatgggt gaaattggtt gctagagtac aaggagttaa ttttacttct atttatggca
     1021 aaacaaaagc tatgggatat cgtagtgtta aattgccata tggacacaca tggaaaagtt
     1081 tcacatattt tttgcttgat accttacctg aaaatataag gaatagctat attaaaaaat
     1141 tttcaacatc tattgagttt tggcagaata caggtggtgg tttgtcagaa atagttatac
     1201 gtgaattgtt gagaaaaggt tataaaatag aattaaatgg agtttcaaat catacaaaag
     1261 ataaaaagag tagagtcata tttttggatg atatacctga tgatacggat gatattaaat
     1321 ttaccaaaga tataccaagt tggaaaagaa tgtgttattg tattttaaaa aatgaccata
     1381 tgtgtagatt tatgggattt ggtcctacaa aagaacagag aattagaacg gaatatatta
     1441 agaagaaata tgaaaaaatt cattagatag gagttaagat gctatggaat ataaaagtcc
     1501 tgtatatgga atcatcgcag tgccaatcga aaagatagaa tctaacgact ataatccaaa
     1561 tgcagtagcc ccaccagaaa tgcagttgtt gtataaaagt ataaaagaag atgggtatac
     1621 aatgccaatt gtctgttatt ataatgagga aaaggataag tatattattg tagatggttt
     1681 tcatagatat agagtaatgc ttgaaaatga ggatatatat gatagagaaa acggacttct
     1741 tcctgtttca gtaattgata aatctcttga tgaaagaatg gcaagtacta ttcgacacaa
     1801 tcgtgcaaga ggttctcata atgtggattt aatgagtaaa attataactg aactttgtga
     1861 aattggaaaa tctgatgaat ggatatcaaa gcacctgggt atggaagttg atgaaattct
     1921 tagattgaag caaataactg gattagctta tttatttaaa gataaagagt ttggtaatgc
     1981 atggattccc tttggctctg atatagaaga agaaaattaa agaga
//
LOCUS       DAEOSP010000315         2019 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.319, whole genome shotgun sequence.
ACCESSION   DAEOSP010000315 DAEOSP010000000
VERSION     DAEOSP010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.319"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            79..1167
                     /gene="dptF"
                     /locus_tag="KRH54_003505"
     CDS             79..1167
                     /gene="dptF"
                     /locus_tag="KRH54_003505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptF"
                     /protein_id="HBG8581133.1"
                     /translation="MRDSVRETLVDILIESTVKYKIIISTRSLLNFIYDFIVNVDLDN
                     KNNKDLIKFVEELSYEAFIKYIFVSNLYNHSELSQILNAVNKIDPINITSENLDNIII
                     KLSNTKDMTILFNQYLNLSKNSYLESSMTNREIIESALNSSKNELSRKILKYNIINTF
                     IRLHIFIPKNNLQLRDNIYISYMRNLYYFNKGEKTYLSNLYDDIKEALYSWNGRIQDD
                     RINMSIGKNQMRYKISQRLNIIPDLSKLRKCTEEELHKFKPSLEIGYKNDEKENQDTC
                     TVDIDFNLYKLLMRVKYGYTPNNNDKYTYINFVVFMNKIHKLGNQNRELYIEDRLEDT
                     VPKYKLTYDKEFDKFKFIRVNGVKVCNL"
     gene            1155..>2019
                     /gene="dptG"
                     /locus_tag="KRH54_003506"
     CDS             1155..>2019
                     /gene="dptG"
                     /locus_tag="KRH54_003506"
                     /inference="COORDINATES: protein motif:HMM:TIGR03236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptG"
                     /protein_id="HBG8581134.1"
                     /translation="MQLIINKEYFKQNYMIDKGIARHNSVKNIVILPYTTTTKEKSKL
                     KEKVERFESCVGEFCRLVYNKKMNIKERQLKDIKLLIKDINVNSSDDSVQLESIIREL
                     FLDEEENMYIFHPKLLNYIHMPSDSYYKKLSKFLYDVLLNSTDNITIKNKINKCFEYE
                     PDNIMELIILKNLPELENEETSKSIYKCICKNVSKLFQEDLEFMLNYPELIINEFERL
                     LKYYYFFYVSQLSIKLSHFFDGDRDRTEKVYFILSWEKASKTRISYNMGWKMLESHIR
                     ILFSHVHTLEIL"
BASE COUNT          897 a          176 c          288 g          658 t
ORIGIN      
        1 ataaatagtt ataaaacaaa ttgtacacat tgtattgtca attataaatg tccgattaaa
       61 aataattatg aaatgttaat gagagactca gtgagagaaa ccttggtgga tatattgata
      121 gaatctacag ttaaatataa aattataatt tcaacaaggt ctcttttgaa ttttatatat
      181 gattttattg ttaatgttga tttagacaat aaaaataata aagacttaat taagtttgta
      241 gaagaactaa gttatgaagc attcataaaa tatatatttg tatctaattt atataatcat
      301 agtgaattat ctcagatttt aaatgcagta aataaaattg accctataaa tatcacatca
      361 gaaaacctag ataatataat cattaagctt agtaatacaa aagatatgac aatactattt
      421 aatcaatacc tgaatttaag taaaaacagt tatctagaaa gttctatgac aaatagagaa
      481 ataatagagt cggctttgaa ttcttcaaag aacgaactga gtaggaaaat attaaaatac
      541 aatataataa atacatttat aagactacat atatttatac ctaaaaataa tttacagtta
      601 agagataata tatatataag ttacatgaga aatttatact attttaacaa aggagaaaaa
      661 acttatttaa gtaatttata tgatgatatt aaagaagctt tgtatagttg gaatggacgt
      721 attcaagatg acagaataaa tatgtccatt ggaaaaaatc agatgagata taaaataagt
      781 caaaggctaa atataattcc agatttaagc aaattaagaa aatgtactga ggaagaattg
      841 cacaagttta agcctagttt agagataggg tataaaaatg atgaaaaaga aaatcaagat
      901 acttgtacag tggatataga ttttaattta tataagttac taatgagagt aaaatatgga
      961 tatacaccaa acaataatga taaatatact tacataaatt ttgtagtatt tatgaataag
     1021 atacataaat taggaaatca aaacagagaa ctatatatag aggatagatt agaagatact
     1081 gtgcctaaat ataaattaac atatgataaa gaatttgata aattcaaatt tataagagta
     1141 aatggggtga aggtatgcaa cttataataa ataaagaata ctttaaacaa aattatatga
     1201 tagataaagg tatcgcgaga cataatagtg tcaaaaacat agttatactc ccatatacaa
     1261 ctacaactaa agaaaaatcg aaacttaaag aaaaggtaga aagatttgag tcttgtgttg
     1321 gagaattctg taggttggta tataacaaga aaatgaacat aaaagaaaga cagctaaaag
     1381 atataaagtt acttataaaa gacataaatg tgaattcttc tgatgatagt gtacaacttg
     1441 agagtataat aagagaatta tttttggatg aagaagaaaa tatgtatata tttcatccaa
     1501 agcttttaaa ttatatacat atgccatctg atagttatta taaaaaattg tctaaatttt
     1561 tatatgatgt tttactaaat agtacagata atataactat aaaaaataaa ataaataaat
     1621 gttttgaata tgaacctgat aatattatgg aacttattat attaaaaaat cttcctgaac
     1681 ttgaaaatga agagacgtct aaatcaattt ataaatgtat atgtaaaaat gtgtctaaat
     1741 tattccaaga agatttagaa tttatgctta attatccaga attaattata aacgagtttg
     1801 aaagattatt aaagtactat tattttttct atgtatcaca attatcaata aaattatctc
     1861 acttttttga tggagataga gatagaactg aaaaagtata ttttatttta agttgggaaa
     1921 aagcatctaa aacaagaata agttataaca tgggttggaa aatgttggag agtcatatta
     1981 gaatattatt tagtcatgtt catacattag agatattaa
//
LOCUS       DAEOSP010000316         2013 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.320, whole genome shotgun sequence.
ACCESSION   DAEOSP010000316 DAEOSP010000000
VERSION     DAEOSP010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.320"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            248..862
                     /locus_tag="KRH54_003507"
     CDS             248..862
                     /locus_tag="KRH54_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBG8581135.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            880..1242
                     /locus_tag="KRH54_003508"
     CDS             880..1242
                     /locus_tag="KRH54_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8581136.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1348..1845
                     /locus_tag="KRH54_003509"
     CDS             1348..1845
                     /locus_tag="KRH54_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBG8581137.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          766 a          221 c          334 g          692 t
ORIGIN      
        1 atactcaaat catgtaaaat ttatataact caattaattt tttaaataca actttataat
       61 ataaaactta taataaaagt aaatttatat gttaaatgag gttgattttt tattattttt
      121 tatttatgct agtactaaag acttgcaaac gttttttgac aagtctttat tttgtctaaa
      181 ttaaacaaac ttttttacaa ataaatacat aaaatttaaa ctaatctatt tattggagga
      241 gataaaattg gaagaagtaa ttgcaaaaat tattactata gaaaatggat tcaaagaaat
      301 tgaaaacatt gctaaaaaaa ttgtaaaaaa taatgactca aaaaagtgtt attatctatc
      361 aatctactta tataggtcag aatattatca agtaagagaa ttggctgtgt ttatacttgg
      421 ttttatatca gtaactattt ctgaagcttt gttattttta aaagataatg tgagtaaaga
      481 tgaaaactgg agagtacaag aaatactagc taaatcattt gattattatt gcagtgaagt
      541 aggatatgaa aaggctcttc ctgttataaa agaatggctt aattctgata gtccaaacat
      601 aaaaagggct gtgacagaag gtcttcgaat ttggactagt agagactatt ttaaatctaa
      661 tcctgacgtt gctatatcac tactttctag tttaaaagaa gatgatagcg aatacttgag
      721 aaagtctgta ggtaatgctc ttagagatat aagtaaaaaa tatccagatt tagtaaaaaa
      781 agaattagat tcatgggata tatcaaataa aaaagttgag tttgtatata agcttgcttc
      841 aaaatgtatc ttggcaaagt aaaatggagg tgaacttatt tgaaacttcg tgatgagtat
      901 acttgcccat tagaactaac acatgatatg ataaaaggca aatggaaatc tattatatta
      961 tggagattac gcataggacc aacttcctta tcaaaacttg aaaaggaaat caatggtatt
     1021 aatcaaaaga tgttacttga acaacttaaa gaattaattg aatttggtct tgtagataaa
     1081 aagacatatg aaggttaccc acttaaagtc gaatactcac ttacacctat tcgagggaaa
     1141 caaatattgg atgctttggc tataatgcaa gatataggaa ttagctttat gttggataat
     1201 ggtatggaag atattcttaa ggaaaaaggt gttttaaagt aaatagatac ccacaaaaaa
     1261 gtaagtaatt taccattatt ttcaactggt atataattaa attatagata tattctatta
     1321 atttattata ataggaggta aattattatg aaagtaacta gtacaggtat agtaaatggt
     1381 gttatagaag ataaatacgg taaaagagga actcagttca atgaaggtgg tatgcctacc
     1441 tattcactac ctctaaagtt tgaagatgct ccaaagaata cagtatcctt tgctgtattt
     1501 ttagaagata aagactctgt tcctgtatgt gggtttgcat ggatacattg gttagctgta
     1561 aatataacaa aagatgagtt aaatgaaaat gaaagtgtaa cagctactga ttttgtacaa
     1621 gggactacta gttggcatgg taaactagga ggtcttgaca gattagaggc cagtgtatat
     1681 ggtggaatgg ctcctccaga cagacctcat gtctatgaaa tacatgtatt tgcacttgat
     1741 actatccttg accttgaaaa aggattctat atgaatgaat ttttcaaagc tattgaaggg
     1801 catgttttag atacatttac actaaaagga agttattcta attaattagg ttgactatag
     1861 ctaatcatta attaaatgct acatttaatt gatgattagc ttaattgtta ttagaatatt
     1921 ttctgcactt tatcttaata tatattttag ttgactggta ttttaattta tattagatta
     1981 ttatcaattt atataaattg attttagctt aaa
//
LOCUS       DAEOSP010000317         1999 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.321, whole genome shotgun sequence.
ACCESSION   DAEOSP010000317 DAEOSP010000000
VERSION     DAEOSP010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.321"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            129..989
                     /locus_tag="KRH54_003510"
     CDS             129..989
                     /locus_tag="KRH54_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBG8581138.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            1037..>1999
                     /locus_tag="KRH54_003511"
     CDS             1037..>1999
                     /locus_tag="KRH54_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBG8581139.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDE"
BASE COUNT          837 a          167 c          348 g          647 t
ORIGIN      
        1 ttataagttt atgtgttatt gtaaactata aattatttag taaaattaaa gtaaaaatta
       61 tagtattttg tggatgaagg tagacttttt tgtaaaatat tattaacata aatatacgag
      121 gtgattagat gagtgaacta tcaggaaaag gctgtgaaat tatagttcct tttgaggaaa
      181 gacttccagt aagagatata gaaaaaagta taataaaaaa atataggaaa aatttatggt
      241 ctaagtttat gaaggcaata agagattata aattggttga agaaggtgat aagatagctg
      301 tagcgatttc tggtggaaaa gacagtattc ttatggctaa aatgtttcaa gagttaaaaa
      361 aacatggaca agttaatttt gatgttgaat ttattgctat ggaccctgga tatcatgcaa
      421 atataagaca attacttata gataattgtg agtatctaaa tataccaata catttatttg
      481 attcaaggat atttgagata gcagatgaaa tagcaaaaga ttatccatgc tatatgtgtg
      541 caagaatgag gagaggagca ttatattcaa aggcagaaga attagggtgt aacaaacttg
      601 cattaggtca tcattatgac gatgtaatag agacaacaat gcttaattta ttgtgtgctg
      661 gaaattttaa aacaatgctt cctaaactta attctactaa ttttgaaggt ataaaaatta
      721 taagaccact ttattatata agagaagaac atattataag atttattcaa aatagtggta
      781 tatggccatt aaattgtgca tgtatggttg ctgctaaaaa aacaggtaat aaaagatatg
      841 agataaaaga cttaataaag agcttagaat caaattttaa aaatgtagaa aaatctatat
      901 ttaaggcggc agaaaatgta aatcttgatt cagtacttgg ttggcaaaaa gatggggaga
      961 aacattcttt cttagaaaat tttgaataaa aatagttaaa aagaatcact ataaacaatt
     1021 ggaaagggtg tttatattga aaagagtata tatagatttt gaaatgaata tgccaaattc
     1081 taaatcaaaa agagcagtat ttaattctga tataattgca atcggagcag ttatgtatga
     1141 tgaaaaaaca aaaaatattg ataaatttaa atctttaata agaccagtaa gtaatgagga
     1201 gttgtatcct catatacaag aattaacaca tatatcttct gaagaattga agagtgcacc
     1261 aagttatgaa gaggtcatga gaaagtttaa aaaatggctt ggcatatttt ctgatattaa
     1321 aggaatatat acttttggta atttggattt gacatgtttc aataacacag atatgagaag
     1381 tgctaaaaaa aataaccatc caaggtttgt aaataacatt agagatttat ttgtagatat
     1441 aaaagaaaaa tatataaatt gtggtatgag atgtatgaac tatatatcat tgaaaaattt
     1501 acttgaattt gttaacttag agtttagtgg agatgcccat gacccattaa atgatgcata
     1561 taatttattt attttggatg aggcaatcac aaataatgta gatatacaaa atcttttaat
     1621 aatacgtgac ataataagac caccatttaa cgatataaat tctaatttag ataattgttt
     1681 taacaaattt aaggagtcat tatataaaaa agaaggaaat tataatattg tagatttttc
     1741 tgttgaaata ataaaaactg tgagaatgta cttattaaca atcatagatg taaatataca
     1801 aaatttagaa attatgaaag atataagtaa aaaaatggat actatagata aattgaaaga
     1861 tatagaggaa ggctattttt atttattaga agatgtgtac ttcgatatga aagatgtact
     1921 agaagattta atgctttata gaatgaatga agatgagtac aagtatgaga taaaaaatat
     1981 aataaaaatg tttgatgaa
//
LOCUS       DAEOSP010000318         1988 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.322, whole genome shotgun sequence.
ACCESSION   DAEOSP010000318 DAEOSP010000000
VERSION     DAEOSP010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.322"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(105..644)
                     /gene="scpB"
                     /locus_tag="KRH54_003512"
     CDS             complement(105..644)
                     /gene="scpB"
                     /locus_tag="KRH54_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBG8581140.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            complement(646..1365)
                     /locus_tag="KRH54_003513"
     CDS             complement(646..1365)
                     /locus_tag="KRH54_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBG8581141.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            complement(1395..1982)
                     /locus_tag="KRH54_003514"
     CDS             complement(1395..1982)
                     /locus_tag="KRH54_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBG8581142.1"
                     /translation="MNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGRMTL
                     NPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAILCV
                     IINKYVHMYAINTIAGYVLLYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLYKFE
                     SMSYIILLIALFTGMYRIILSPIYTVLLKIVYLFV"
BASE COUNT          626 a          329 c          184 g          849 t
ORIGIN      
        1 gagaattata attttatatt tatacaaaaa aacacaacaa cttagagcat tttaaatctt
       61 ttaaaattta aaattacata ctaaattatt gtgattttat atttttattc atttgttgcc
      121 acttcttgat aattttctat aggtggcaat tcttcaagac tttctatatt caacaacttt
      181 aaaaattcat ctgttgtttt ataaattatt ggtttcccta tcttattcaa tctaccagct
      241 tctcttataa gttcattttc aaacaaagtt tgtaatacct tatcacattt tactcctctt
      301 atatcctcta tttctacctt tgttattggt tgtttgtagg caattatagt aagagtttct
      361 aatgtagctt ggctcaaact tttctttttc tttggttcaa taatcttctt tatatattct
      421 gcataatctt tatttgtaca catttggtac ttattctcta acttgattat ttgaatacct
      481 ctattttgtt ctctgtactc ctcaataagt aaatttaaca taatctctat ttcttttgat
      541 gaaagttctt tatttattat atagtttaat tcttttatac ttattggttc cccatatgca
      601 aacataacag attctataat atactttata tcttctcttt tcattttaac actccgaact
      661 ttttcttatt agaatatcat caaaaaatat atcttgtact acgactattt ctttttcttt
      721 tataagctct aatatagata aaaaagtagc tattatttca tcattttcat atgattttat
      781 aatatttgta aaagtaactt caatcttatc ttttataata tctcttatat ataatatttt
      841 ttcttctaca gatattattt tcttccttac tattttatct agtttctcat cattagcatt
      901 ttctatctgt gatgttttaa ctttaaatat gtatggaagt atatttttta ttgcttcaat
      961 tgatatatct tctaaatcca cattgtcatc aataattatc tcttctttat ttctataaaa
     1021 tctctcatta acataagtta tattttcttt tatatcttgt gatgctacct taaactttct
     1081 atattcttct aacttttcca taagttctat tcttgggtct tcttcttcat ctttttgctt
     1141 ataaagcaga tatttagact ttatctctaa aagttttgat gccatagtta taaattcact
     1201 agttatctct aaatccattt tatctagcat tttcaaataa tttagatact gcttagttat
     1261 atctattatg gatatatcct ttatatctat tttatgcttt gttattaaat cataaagtaa
     1321 atctaatggt ccctcataga cttgtaattg tatattatac ttcatatcaa tccctcttta
     1381 atctgtttat tatattaaac aaataaatat actattttta gcaacactgt atatattggt
     1441 gatagtatta ttctatacat cccagtaaat aaagcaatta ataaaattat ataactcata
     1501 ctttcaaatt tatacaaata ttcattccat ttatatggta caaatgtaga tataactccc
     1561 catccatcaa gtggaggtat cggaagtaag ttaaaagctg caaatcccat actatataaa
     1621 agcacatatc cagctattgt atttatagca tacatatgca catacttatt gataattaca
     1681 cacagtatag cagtcaaaac attacaaaaa acgcctgcaa gtgaaactat caggtttcct
     1741 atcttatagt ttttaaagtt atttggattt actggtactg gctttgccca ccctatctta
     1801 aggataaaca tagccaaaag acctattggg tcaacatgtc ttacaggatt taatgtcatt
     1861 ctaccataag ctttagctgt atcatccccc aataagtgag ctgaataagc atggccaaac
     1921 tcatgtattg atattgccat agctatacca actaaacttg ctactatttc tcctatattc
     1981 atacttaa
//
LOCUS       DAEOSP010000319         1974 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.323, whole genome shotgun sequence.
ACCESSION   DAEOSP010000319 DAEOSP010000000
VERSION     DAEOSP010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.323"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            37..1965
                     /locus_tag="KRH54_003515"
     CDS             37..1965
                     /locus_tag="KRH54_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBG8581143.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSENSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
BASE COUNT          781 a          193 c          276 g          724 t
ORIGIN      
        1 tataatttag aagaaattct ataagggggg atatttatgc acaagagatt gctcacgttg
       61 tttaaattat taaatgaaag tgatgataaa attacttgta aaactttaag taatcattta
      121 aaagtgagtg agagaactat aagaaatgat attacttcta taaatggaac tttagagaaa
      181 aatggtgcta ttattaaaat aaaaaaagga gaaggatatt atatagatat tcttaattta
      241 gcattatacc aacagtatct agcattaata tctgatgata ttatggattc aagtgaaata
      301 ccagattctc ctatagaaag aaatcaatat attttaaaat atattctcta taacaataca
      361 tatataaaac tagaagattt agctaatagc ttgtatgtga gtaaatttac aatactaaat
      421 gacataaaaa gaataaagcc tattttgtca aagtataatt taatattagt ttctaagcca
      481 tattatggag ttaaagttga aggtaaggaa atagatattc gaagatgtat ttctaataat
      541 atgataaata ggaattttga aaactatata attggaatta cagatagaga aatagaatta
      601 tttaataatg tagatttaat agagcttcaa agggtagtgt taagtgaaat taataaattt
      661 aatatcaatt tcctagattt taatctaaaa aactttatta tccatttagc tattactgta
      721 agtagaatac ttgatggtta ctgcttagat aatgttttag atgttgtttt aacagatttt
      781 caatcgaata caactgttga aaatatattt aattatattg agagtaaata tactataatt
      841 atttcaaagg cagacagagt ttatctatat aatcatttta taactaaaag ttctcttttg
      901 gataacgttt ccaatagggt agatactaaa attatagagt atgttgaaga aattttagaa
      961 gtaatcaaca atcaatatac ttttgacctt agaaatgata gtgtattatt tgatgattta
     1021 gtactgcact ttaaatcaat tctcaattct aaatcttata atttaaataa ggtgaatccc
     1081 cttataaata caataaagag taattatcct ttagcatttg aaataacttt aaatgctata
     1141 gaaaaagttt ttaaaaatag tatttactct ttaacagaag atgaaattgg atatgtatcc
     1201 ctgcatatag gtgctggaat agagagattt tttcaaagta atattaagtg taaaaacgta
     1261 gtattagtat gtggttcagg gtatggttca agtaggttat tagaggttca attaaacaaa
     1321 gtttttcatg ataaaataaa tatacttcaa tgtctatcat ttaatcaatt tttagctagt
     1381 gaattgtcag atgtagatat aataatatcg acaattcctt taaatcatga tagtattcct
     1441 attgtattag tggatttaaa actattaaaa aaagatatag agaatatatc taagtcaatc
     1501 actaataatt cccatattta tagtaatctt ttggacaatt tctttgataa aaatttattt
     1561 attgttaatc ctaaaatcaa agataaagat gagcttataa agttgatgtg caacaaatta
     1621 actcaaagtg aaattgtatt tccatcattt gctgaatctg tattttacag agaaagttta
     1681 tcttctacca atatagatga ttttttagct atacctcatc ctatggaatt aagctctata
     1741 agaactaaga tttgtatttc catattgaat gaaccaatat attggagtga aaattctact
     1801 gttaaattaa tcatgatgtt ggctataaat aaagatgatt atataaaaat aaactccatt
     1861 tatgatatcc ttttaaagat aattcatgac aatgatatta gagatagtat atctaattgt
     1921 aatgattttg ataattttct ttccataatc aagtctatag tatagatttt tata
//
LOCUS       DAEOSP010000320         1958 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.324, whole genome shotgun sequence.
ACCESSION   DAEOSP010000320 DAEOSP010000000
VERSION     DAEOSP010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.324"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            131..1636
                     /locus_tag="KRH54_003516"
     CDS             131..1636
                     /locus_tag="KRH54_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBG8581144.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
BASE COUNT          779 a          209 c          350 g          620 t
ORIGIN      
        1 aattaatctt aattgtataa tattactttg agtgaaaaag tattaacatt tttttataag
       61 atgttttact aaaaaaatat atataaatat actgttatca ataagaaatt taatttagtg
      121 aggtgttaag atgaaagtag gatttgacca caaaaaatat ttagaagagc agtctaaata
      181 tatcttagaa agagtaaata attttgacaa actttattta gagtttggag gaaaattgat
      241 gggagacctt catgcaaaaa gagtacttcc aggatttgat gaaaatgcaa agataaaagt
      301 attacaacat atgaaagaaa aagttgaagt tgtaatttgt gtctatgcag gagatataga
      361 aagaaacaaa ataagaggag actttggtat cacttatgat atggaagttt taagattaat
      421 agatgattta agaggttatg aactagatgt aaatagtgta gtaattacta gatttgaagg
      481 acaacctgct acaactgtat ttataaataa attagaacgt agaggaatta aagtttataa
      541 gcattatcca acaaagggat atccgtcaga tgttgagact atagttagtg atgagggata
      601 tggagcaaat gaatatatag aaactacaaa gccaatagta gtagtcacag caccaggacc
      661 taatagtgga aaacttggta cttgtctaag tcagttgtat catgaaaata aaagaggtaa
      721 tgtagttgga tattctaagt ttgagacatt cccagtttgg aatgtacctg taaaacatcc
      781 tttgaatata gcttatgaag cagctacagt tgatttaaaa gatgtgaata tgatagattc
      841 attccacctt gaaacgtatg gtgaaatgtc agtaaactac aatagagatt tggaattatt
      901 tccagtgctt aaaaaaataa tagaaaagat aactggaaaa gaatcaattt tccaatctcc
      961 aactgatatg ggtgttaata gagttggctt tggaataata gatgatgaag taataagaaa
     1021 agcttctata gaagaaataa taagaagata ctttaaaact gcctgtgagt ataaaaaggg
     1081 acaagttgat aaaggtgctt atgatagaat gaaaatgatt atggaagagc ttaacttaaa
     1141 gccagaagat agaaatgttg tattacctgc tagaaactac agtaataaat tgaaagaaag
     1201 tgccgacaag aatgatactt gtcctgtagt tgcattagaa ttagaagatg gtacaatatt
     1261 aactggtaag ggttctgatt taatgaatgg aaccgcagca gtagtattaa atgccattaa
     1321 acatttagcc aatatatctg atgatatgca cttaatatca cctgtaatat tagaacctat
     1381 cataaactta aaaacaaaga ctttagctag tagaaatgtt tcacttagtt gccaagaggt
     1441 gttaactgca cttagtattt gtgctgtaac taatccaact gcacaatttg ctatggaaaa
     1501 gttagcatta ttaaaagggt gtcaagctca ttctactact atattaaata gagatgatga
     1561 acaaacattt agaaaacttg gtatagatgt aacttgtgac ccagaatatc cttcacaaaa
     1621 tctttactat agttaaaatt atataaaaaa atatgggctg ttttaaagta atagtttgag
     1681 cagtccatta caattataat aaaatcagta ggctgtcttg tataagacag tcttttgttt
     1741 ttaaaacatc ccataaatta aatatatata ctgttatcca taaggaattt taaatttagt
     1801 tccttggaat acctattgta aagcttttat atagtttttg caagtgtttt taaggatttt
     1861 ttataaatta tattgaagtt aatggattat agatggtttt atttatggaa aatatgaata
     1921 tataaagaaa ataatgaaaa aataccaaaa agaaacat
//
LOCUS       DAEOSP010000321         1957 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.325, whole genome shotgun sequence.
ACCESSION   DAEOSP010000321 DAEOSP010000000
VERSION     DAEOSP010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.325"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(36..1253)
                     /locus_tag="KRH54_003517"
     CDS             complement(36..1253)
                     /locus_tag="KRH54_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBG8581145.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTTDLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(1444..1857)
                     /locus_tag="KRH54_003518"
     CDS             complement(1444..1857)
                     /locus_tag="KRH54_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8581146.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT          668 a          323 c          223 g          743 t
ORIGIN      
        1 gattatatat tttaatatac tttcttatat aagaattatt ttaaatcaag attatctagt
       61 atttttaaaa atatttttac agcagtatcc aagtcatcta cagaaacact ttcattagga
      121 ttatgactta cgcccccagc gcatcttata aataccatac caatatcagt tatattatcc
      181 atttcctgag catcatggcc agcaccacta taaatataaa aaggattaag atttaaatct
      241 ataaaacttt tttctatgat ttttgttatt ttattagaac atggtacagg aacattttca
      301 aaagcaagtt cacttgtata attaagtttt ctattttcac atatatgtga tatttcatta
      361 aatatctttt ctacagaatt atcaagaatt tcttgtgaac aagagcgtac atcaagagta
      421 aattcagctc tttcaggtac cacatttgat gaagatggat agaaattcat ctttcctaca
      481 gtagcaacca aatcagtagt cgttttagct actttttcga tagctaatac acattcacag
      541 gcacaacatc cagcatcaag tctcatattc attggaagtg taccagcatg tccagactta
      601 ccatttactg atatcttaaa acttttaaat cctgtaatac ttgatacaat tccagcagga
      661 agagattcat tttctaaaac aggaccctgc tcgatatgaa attctaaata tgcatctaca
      721 tcttttctag gatgcaaatt tgttaagttt tttgtattaa atccaaactt tgttacagct
      781 tcttcaagtg taattccatt ttcatcaaca gatttaagaa gttcctcttt aaaagtacca
      841 gcaaaaactt tacttcccat taaagaagtt tcaaatctag ttccttcctc ttcagcgaaa
      901 gcaattagct taatattgaa aggataagac ctattattgt gttttgcttc ttcaagacca
      961 actagaggca ctattatacc taacatacca tcaaatatac ctccacactt tacactatct
     1021 tgatgagatg caactactaa tgtgggagca tttggaaagc ttgatttata tgtacctata
     1081 acgtttccaa tgtcatcaat ttcaatatca agatttaaac cttccatcca tgaactaatt
     1141 aaatcaatag attttttatg ttcttgagtc aagataagtc ttgtaagacc atttgttgtt
     1201 gaacttattt ttcctaatgt ttgtagataa tccatacatt tttttcctaa catatttacc
     1261 tcctaaaaat ataattttta tttaagctaa gattagcttt tatacttata atttttgaac
     1321 tttcaaataa ttttgtatgt atagtaaaat actattttag tattaataat gatataaaaa
     1381 cataaagtct gtagacgaag agcagaagtt ttaatttaaa aatatgaaat atgattttta
     1441 ttattatagt atttttctat aataatatct ctttccagtt agaggataat tttcaagtga
     1501 aaatacttta tggtatccaa atttctcata aaatttaggt gcttgaaaac tgaatgtatc
     1561 taaaaaaaca tatttacaac ctcgttcttt agcaattttt tcagccttat ataaaagttg
     1621 acttccagta cctttatatc taagcttctc actaacccat aagtatttta tagaaagcca
     1681 attaccatga gtaacaccta taaaacctgc aacaatttta cctgctttat ctcgaatata
     1741 tattcctaaa tccactgggt ttttatcttc aagtctagca agattatatt ttaataatcc
     1801 ttgaaaaata gtatcttcat cttgtttttc tattttttct gtaatttcta attccatatt
     1861 tgaactttct ccttgtataa tcttttgtat attggtattt tttaataaaa tcagtagaaa
     1921 gaatatagtt taacattatt ctaataggca tttatgc
//
LOCUS       DAEOSP010000322         1939 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.326, whole genome shotgun sequence.
ACCESSION   DAEOSP010000322 DAEOSP010000000
VERSION     DAEOSP010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.326"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            269..553
                     /locus_tag="KRH54_003519"
     CDS             269..553
                     /locus_tag="KRH54_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581147.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(710..883)
                     /locus_tag="KRH54_003520"
     CDS             complement(710..883)
                     /locus_tag="KRH54_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HBG8581148.1"
                     /translation="MKRKLSTYFKGYILGLCIAFLLFTFILKKLIIENIIGIIIGTGI
                     AFLLIYLFNKKRK"
     gene            complement(1324..1539)
                     /locus_tag="KRH54_003521"
     CDS             complement(1324..1539)
                     /locus_tag="KRH54_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8581149.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(1550..>1939)
                     /locus_tag="KRH54_003522"
     CDS             complement(1550..>1939)
                     /locus_tag="KRH54_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG8581150.1"
                     /translation="IFLCMIPMFLYDFSSDKVIALIARILYFSDYFIVILFLIFIINS
                     TKYSPFVLENVKRFKVMGCCLLINTIIECINGYNLNTNNIRIFGTDSGGISPLMVISF
                     IFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT          775 a          207 c          239 g          718 t
ORIGIN      
        1 ttggcgtttt atattaacta tatggaatgt aaatacaata cccccctatt gcaatgcttc
       61 attaactatt agttttatat taactatatg taatataaat gttttttctt attaaataaa
      121 gattgagtca ctttatattt aataatacat attctaaatt gattttgttt ttatataaga
      181 gaatttgaat aatatggaaa aagatgatta ataaattatt aattagggta taaaatgata
      241 taaatagaat aaataagggg tggatgaaat gcttgtatat aataaaagtt tttatcctaa
      301 tgacatattt ccaagattag atttttcaaa aataaaaaaa cagttaaaat tgatagataa
      361 tgacctgtca gattttggaa gcatatgtat aatagaaaaa gaacattata caataagtgt
      421 aaacagtata ggtgaaataa atgtgtatta tgatttagag tacgaaaata aggtgtatag
      481 aatagtttat gagattgaaa agttatttaa atctcaagtt ggaaggttta gtatatctac
      541 atacagaaat tgataattaa aaaagtagaa attaaaaaac ttaatactaa agatatagat
      601 aaatatatga ggtaacagct agagagagtt gttacccttt ttaaatacta agtatatatt
      661 attcttatta attttttgta aattcactaa aagacctttt tatagaatat tattttcttt
      721 ttttattaaa taggtaaatc agtaaaaatg caattccagt tccaattatt attccaatga
      781 tattttctat aattaatttt tttaatataa aagtaaataa taaaaatgct atacataaac
      841 ctaaaatata tcctttaaaa taggttgaaa gttttctttt catataatca ccaacttgta
      901 tcagatttag tttacttaat tagagtataa cagtgttgta acttcaaaga gtatctaaaa
      961 gattacaaat gttagataaa atcatcaata atgtaattta taacacgtag ctataaacta
     1021 tgcaataata taaatggagg tataagtaaa tgaaaaagtt ccatgttttg ctaacagttt
     1081 aaacaaatat taggtagaaa agaatgtata taggaatctg tggtaaaatt atcaatttaa
     1141 aagtatgatt tagtgtgata cagttcttat aggtaatgga taacaaatag ataaaggaac
     1201 agtttctttc ttagaacata ttttgttatg actttaaaca aagttaatat taatgtcaat
     1261 aatatagtaa aagaataaaa atcatatagt ataataaaaa agagtgtttc taaacactct
     1321 tttttaatca ttttcatctt ctacatattc aagtatatca cctggctgac agtctaaagc
     1381 tttgcaaatt tcgtttagag tggtaaatct aatagctttt gcttttccag tttttaaaat
     1441 tgataaattt gcatttgtaa tatctatttt ttctgccaaa tctttaagtg atattttcct
     1501 ttttgccatc attacatcta ggttaactac tattgccata acatcacctc tatattgtta
     1561 aatcattatc ttcttttatt tttatagctt tatcaaatgt ttctgcaatt acaaagcaca
     1621 ttagtgcaaa tataaaagag ataaccataa gtggtgagat accgccacta tcagttccaa
     1681 aaattcttat attattagta tttaaattat aaccatttat acattcaatt atagtgttta
     1741 tcaataaaca acatcccatt accttaaacc tttttacatt ttcaagtacg aatggggaat
     1801 acttagtact attaattata aatatcaaga ataagatgac tataaagtag tcagaaaaat
     1861 atagtattct tgcaattaaa gcgattactt tgtcagaact aaaatcatat aaaaacatag
     1921 gtatcataca taagaatat
//
LOCUS       DAEOSP010000323         1867 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.327, whole genome shotgun sequence.
ACCESSION   DAEOSP010000323 DAEOSP010000000
VERSION     DAEOSP010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.327"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(319..1611)
                     /gene="eno"
                     /locus_tag="KRH54_003523"
     CDS             complement(319..1611)
                     /gene="eno"
                     /locus_tag="KRH54_003523"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBG8581151.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT          578 a          364 c          191 g          734 t
ORIGIN      
        1 caatcatttt aaaatattga tataactttt aatttcaaca taatttaaaa taaataatcc
       61 acatttagca ataatacttc aatgacaatt atgattaaat caaaatatgt cttcactttt
      121 attttttaat aaaaacctcc tatgcaatta gataaaaatg agatagaaac atttattcat
      181 attttttctg aatctatgaa taaaattaag taataaaata taaaaaagga taagtgaatt
      241 gaaaaatcaa ttaacactta tctcttttat atcttatatt ctctcttcat taatcttttt
      301 tagtaaccat tttaataact atttttttaa gttatagaaa gattttaatc cacaatatct
      361 agcttgctct ccaaccattt cttctattct taataattgg ttgtacttag caactctatc
      421 agttcttgat ggagcaccag tttttatttg accagcattt actgcaacag ctaaatctgc
      481 tatagtagaa tcttcagttt ctcctgatct gtgagatata acagcagtat atcctgctct
      541 cttagccatt tctatagcat ctaaagtttc agttattgta cctatttgat taactttaac
      601 taatatagag ttagctactc cattttcaat tcctttttct aatctctctg tgttagtaac
      661 aaataagtcg tcaccaacta attgtaattt attacctaat ttttcagtta ataatttcca
      721 tccatcccaa tcttcttcgt ctaaaccatc ttctatagtt attattggga agttgtttga
      781 ccaatcttca tataaagcaa ccatttcagc agcagttaac tcttttcctt ctcctgctaa
      841 aacgtatttt ttagtttctt tgttgtacat ttctgtagca gcaacgtcaa gacctaacat
      901 aacatcttca cctggcttat atccagcttt tgttatagct tcaactatta attctaaagc
      961 ttctctgttt gaacctaagt ttggagcaaa tccaccttcg tcacctacac cacaagctaa
     1021 tcctttttct cctaaaactt tctttaatga atggaatact tctgctccca ttcttaaacc
     1081 ttctttgaaa caacaagctc ctactggtaa tatcataaat tcttgaacat caacgttgtt
     1141 atcagcatgc tctccaccgt ttaatatgtt catcattgga acaggtaatt gtttagcatt
     1201 tactccacct aagtattgga ataatggtaa acctatttca tcagcagctg ctctagctac
     1261 tgccattgaa acacctaata tagcattcgc tcctaattta cctttgtttg gagttccatc
     1321 taattctatc atgattgcat ctatagctgg ttggtcaaat gcatccattc cttctatttc
     1381 aggtgctatt atttcattta cgttagcaac agctgtttct acaccttttc ctaagtatct
     1441 tcccttatct ccatctctta attcaacagc ttcaaaagct cctgtagatg ctcctgatgg
     1501 aactattgct cttcccattg ctccatcttc taatactact tcaacttcta cagttggatt
     1561 tcctcttgag tctaatactt ctctagcata tactaattca ataactgaca tttgtatatc
     1621 tcccttcgat tttctattag ttaaattttc atttactgta aacatccgtc tactatttta
     1681 atctactttc tatattatag tacataaagt tgcttttgaa aattaggtac aaatttattt
     1741 gatgggtaaa tttgtaaaag ttcctatttt tattttattt tttataatat tttaatttat
     1801 ataatataaa aaaacttgtc taaaatccaa aaccaatctt aaatacacaa atattataac
     1861 tagattt
//
LOCUS       DAEOSP010000324         1853 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.328, whole genome shotgun sequence.
ACCESSION   DAEOSP010000324 DAEOSP010000000
VERSION     DAEOSP010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.328"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..1716
                     /locus_tag="KRH54_003524"
     CDS             <1..1716
                     /locus_tag="KRH54_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C80"
                     /protein_id="HBG8581152.1"
                     /translation="STGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNI
                     EGQAILYQNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINND
                     KYYFSYDGILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEG
                     QAIVYQNKFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKY
                     YFNLNTAEAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGP
                     NGFEYFAPANTDANNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFN
                     TNTAVAVTGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGF
                     EYFAPANTDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNT
                     AMGANGYKTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFL
                     HLLGKIYYFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKA
                     PGIYG"
BASE COUNT          665 a          238 c          309 g          641 t
ORIGIN      
        1 tcaactggtt atacaattat taatggtaaa catttttatt ttaatactga tggtattatg
       61 cagataggag tgtttaaagg acctaatgga tttgaatatt ttgcacctgc taatacggat
      121 gctaacaaca tagaaggtca agctatactt taccaaaatg aattcttaac tttgaatggt
      181 aaaaaatatt actttggtag tgactcaaaa gcagttactg gatggagaat tattaacaat
      241 aagaaatatt actttaatcc taataatgct attgctgcaa ttcatctatg cactataaat
      301 aatgacaagt attactttag ttatgatgga attcttcaaa atggatatat tactattgaa
      361 agaaataatt tctattttga tgctaataat gaatctaaaa tggtaacagg agtatttaaa
      421 ggacctaatg gatttgagta ttttgcacct gctaatactc acaataataa catagaaggt
      481 caggctatag tttaccagaa caaattctta actttgaatg gcaaaaaata ttattttgat
      541 aatgactcaa aagcagttac tggatggcaa accattgatg gtaaaaaata ttactttaat
      601 cttaacactg ctgaagcagc tactggatgg caaactattg atggtaaaaa atattacttt
      661 aatcttaaca ctgctgaagc agctactgga tggcaaacta ttgatggtaa aaaatattac
      721 tttaatacta acactttcat agcctcaact ggttatacaa gtattaatgg taaacatttt
      781 tattttaata ctgatggtat tatgcagata ggagtgttta aaggacctaa tggatttgaa
      841 tactttgcac ctgctaatac ggatgctaac aacatagaag gtcaagctat actttaccaa
      901 aataaattct taactttgaa tggtaaaaaa tattactttg gtagtgactc aaaagcagtt
      961 accggattgc gaactattga tggtaaaaaa tattacttta atactaacac tgctgttgca
     1021 gttactggat ggcaaactat taatggtaaa aaatactact ttaatactaa cacttctata
     1081 gcttcaactg gttatacaat tattagtggt aaacattttt attttaatac tgatggtatt
     1141 atgcagatag gagtgtttaa aggacctgat ggatttgaat actttgcacc tgctaataca
     1201 gatgctaaca atatagaagg tcaagctata cgttatcaaa atagattcct atatttacat
     1261 gacaatatat attattttgg taataattca aaagcggcta ctggttgggt aactattgat
     1321 ggtaatagat attacttcga gcctaataca gctatgggtg cgaatggtta taaaactatt
     1381 gataataaaa atttttactt tagaaatggt ttacctcaga taggagtgtt taaagggtct
     1441 aatggatttg aatactttgc acctgctaat acggatgcta acaatataga aggtcaagct
     1501 atacgttatc aaaatagatt cctacattta cttggaaaaa tatattactt tggtaataat
     1561 tcaaaagcag ttactggatg gcaaactatt aatggtaaag tatattactt tatgcctgat
     1621 actgctatgg ctgcagctgg tggacttttc gagattgatg gtgttatata tttctttggt
     1681 gttgatggag taaaagcccc tgggatatat ggctaaaata tatgtttgat aaaaaattat
     1741 tcctgtgcta ctaagaaatt atttttatat aataaatatt gagatttaat taaaagtcat
     1801 gtgttattgt aatacatgac ttttagttaa aatttttcta tcatttaata atc
//
LOCUS       DAEOSP010000325         1852 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.329, whole genome shotgun sequence.
ACCESSION   DAEOSP010000325 DAEOSP010000000
VERSION     DAEOSP010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.329"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(204..452)
                     /locus_tag="KRH54_003525"
     CDS             complement(204..452)
                     /locus_tag="KRH54_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG8581153.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVNDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            complement(483..944)
                     /locus_tag="KRH54_003526"
     CDS             complement(483..944)
                     /locus_tag="KRH54_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG8581154.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRFLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            complement(982..1815)
                     /locus_tag="KRH54_003527"
     CDS             complement(982..1815)
                     /locus_tag="KRH54_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8581155.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
BASE COUNT          658 a          271 c          209 g          714 t
ORIGIN      
        1 ataatgtcca attcatatat gcaaaattta tatactttaa aatatctaag aaattattca
       61 ataaaaaaat actatttttt aaaaattatc attattgata aagctctact atttatattt
      121 tttagtgtat tcatgtataa taagtaaaag cacaacggta tatactgttg tgcttttttg
      181 taattaataa acaatatttt ggtctataac aatgaaaaat ggctactttc cataattgat
      241 ttcatctgac ataagcaaaa agggtgtcca gctgggtcaa tcataactct ccagtcatca
      301 gtaaattgct cctctgcgat tgtagctcca caatgaattg catattgaac tgcttcttct
      361 aaatcattaa cagcgaaatc catatgagcc atttgctgtt gagattcagg tttttctggt
      421 tctattatgc cccaagcctt tgttttttgc aatataaaga aaaaattata tattttaata
      481 ttttataatt gttgaaattg tgtaataaat tcaactggat acatagtgcg ttttgaaatt
      541 tcttctaaag ctaaaccttg ttttaaaaac ctctttatga cgctttccat actttctgaa
      601 atttctataa tatttttatc aaaatcataa aacctaaaag ttctttgacc ccatgggtat
      661 tcttttatat aatgcaaaaa ttcaatattt cctattctat ttatcttact ttcccattgt
      721 ttgatgtcat taacctcaaa atacaactga aagttatttg attgttgata tattggtaaa
      781 tcatatccaa ttatagcttt ataattactt tgtaatccaa atccattttc aaatgataca
      841 tgttctccta aatctaatgt gattttttgc tccataacat cttcataaaa cttttttgat
      901 ttatttatgt ctttgacaat tagcaatact cctccaaatt tcatcttatc actatccttt
      961 ctttgttata agtattataa cttaaaattt tgcaatttca ctgtaaaaat cagacatatt
     1021 agctcggtat ttttgaggag ttattccaca gacaatttta aaatctttaa taaaatgtgg
     1081 tatatcatag aatcctaatt cattacatat agacatcaat gaagttttat tattgtttag
     1141 aagttttata gatttattaa tacgtaccaa acgagaaaat gacttcatac tcaaccctag
     1201 gtactgatta aagagtctgt ttaaatgcct agaactataa aatacttttt tagaaatttc
     1261 ttgtgaagat atatttccag agttttgaat aatcaactga attgccaaaa taaactcata
     1321 tgagtattca attttaatat tagaaatcaa taattcttcc aactttaaaa tcaatttatc
     1381 tatagataaa gattcattaa atattttttt aatttctaaa tctaatgaag tattaattat
     1441 agaaaatgga acaactttat caataagttc actttgctgt atacctataa gcgggaaaaa
     1501 accagcaggt tgaaattcta taattagtat aacatcacat ctattagcaa tatttcctac
     1561 tgtttttggt ttagtagttg gaccaaataa tagactatgt aaccctgtgg catcataaaa
     1621 aaacaccaaa gttacacttc catgaggaat aatcgtgtaa ttatcagaaa ttattgtttg
     1681 atttggaaac gatatagtaa aattcgatat ccaatttctt aattgtatac aaggcaagag
     1741 ataaacaaaa ttgtcttcac aaactataag tgacctattt ttatttataa aatgaatact
     1801 ttcttttttt aacatatatt atatcctgtc ctatcgtgaa tatttattaa ag
//
LOCUS       DAEOSP010000326         1850 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.330, whole genome shotgun sequence.
ACCESSION   DAEOSP010000326 DAEOSP010000000
VERSION     DAEOSP010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.330"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            200..1798
                     /locus_tag="KRH54_003528"
     CDS             200..1798
                     /locus_tag="KRH54_003528"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBG8581156.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
BASE COUNT          709 a          222 c          284 g          635 t
ORIGIN      
        1 atttaattcg atatttgatt gggtaaaagt atatataata ataattttta tattaaattt
       61 gtagtattta tgtaagaaat aaattttata taatattttt tcactaaatt tttttctttt
      121 tacaacaaat actcactacc ttttgatata atatttttat caattattat tatattcaag
      181 ttttgaaagg agtattaaaa tgagtttatt agttttcgga cataaaaatc cagatacaga
      241 ttcaatatgt tctgcaattt cattagcaca tttaaagaac aaactaggaa taaaagcaac
      301 agcttatgct ttaggagata tcagaaaaga agctaaatat gccttggatt actttaaggt
      361 agatgcacct gaaattttag ataatgttag aatacaagtt agagatttaa attatgataa
      421 agtagtacct ttaacaccaa cttcatctat attagaggct tataacctta tggatgaaaa
      481 aaatgttaaa acactacctg taacttatga tgatggaact tttgctggta tcattactat
      541 gaaagaaata gctaaaaatc tacttcatca acactttaca actatacaca catcactatc
      601 aaacatttgt aaaaacctta atggtactat tcttgtggac tgtggtgaag atataaaagg
      661 tagagtagtc actttatcat ttggaatgga gactctaata gagactttaa aagaaggaga
      721 catagctatt gttggagata gatatgatag tattgaatat gctattgata caaaagttaa
      781 acttttaata cttactaatc atacagagat ttcaaaagat ttgctagctc ttgctaagat
      841 aaatggagtg tctgttgttt ctgttgaaag tgacaattat aaaacttcta atgtaataaa
      901 tcaatgtagt ttcttagact ctatagaagc tacagaaaat ttaattacat tcaaagaaga
      961 tgattatatg gatgaaataa aagagattat gctagaaaca aacttcagat cttatccaat
     1021 attagatgat gataataagt ttttaggttt agtttcaaaa ggtcatctat taaacccatc
     1081 taaaaagaat gttgttttag ttgaccataa cgaatatgct caaagtgctg atggaattga
     1141 acaagcaaac atagttgaaa taattgacca tcataaactt ggtggtattt ctactgatgt
     1201 tcctatgtca tttagagtta tgcctgttgg atgcaatagt actataattt atcaaatgta
     1261 caaagaaaat aatgttgaga taccttatga aatagccggg cttcttttat ctgctatatt
     1321 atcagataca ttattattta agtctccaac aacaactgat atagataaga aagcttgtga
     1381 agaattaagt aaaatagcaa aagtagacat ggaaaaatac gctatggata tgtttaaatg
     1441 cggtacttct ttagatgaat atactataga agaaatagta aatatggact ttaaagaatt
     1501 taatatgagt ggacatagag ttggtattgg acaagtattt accttagaca tagactctat
     1561 atttgctaaa aaagatgagt ttttatctta tataaattcg actgattatg acaaactaat
     1621 tcttgcggtt acagatataa tcaaagaagg ttcttattta atctataaag cagaagacag
     1681 cttaattgct aacgcatttg gtgttgaagg tgttcaaggt acatttgcac caggtgttgt
     1741 atcaagaaaa aaacaattag tccctaattt aactactgca atcaaaaatt ttaaataata
     1801 taaaattttt aaataacgaa aactgtctca aatctatatt tgagacagtt
//
LOCUS       DAEOSP010000327         1807 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.331, whole genome shotgun sequence.
ACCESSION   DAEOSP010000327 DAEOSP010000000
VERSION     DAEOSP010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.331"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     misc_binding    103..308
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            434..1705
                     /gene="serS"
                     /locus_tag="KRH54_003529"
     CDS             434..1705
                     /gene="serS"
                     /locus_tag="KRH54_003529"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG8581157.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
BASE COUNT          731 a          173 c          340 g          563 t
ORIGIN      
        1 atttaatata aaaaattaaa gaaaattcta aaaaaatttg aataatagta ttgaaagatg
       61 ttaatatata taatataatg attatcaaat aaaacaatta gttaaaaagc tgtgaaggaa
      121 aatagtaaaa aacagattgt attcagagaa tgagtggatg gtgaaaactc attacaatgg
      181 tttttgaatc tactctagag cttctagtta aagctagacg tttacctacg ttataggtct
      241 gagaggatta tacattttag tatagtcaat tagggtggca acgcggataa aagattttcg
      301 tcccttttat agggtaaacg gaggtctttt tttattttta aaatttgttt aaactaagag
      361 tttacaagta taatgtttgt aaattaagtt ggaattataa cttatattat caaaattatt
      421 aggaggaatt aatatgttag atattaaaag aataagagaa aatctagatg atatcaaaaa
      481 agctatggaa agaagaggtg aaagagaatt tgaccttgat gctgtagtgg aattagataa
      541 taaaagaaga gaaatacttc aagaagttga agttatgaaa aatgagttaa atgtaaattc
      601 taaaaaaata cctcaattaa taaaagaagg aaaagatgta acagaagaaa aggcaagatt
      661 gaaagagtta tcagataaga taaaaggtat tgatgagaaa gtaaaagaag tagaagcaaa
      721 aatggaatat acattaatga gaataccaaa tgttcctcat ccagaggtac cacaaggtga
      781 aacagatgaa gacaatgttc aaataagaac ttggggagaa cctactaaat ttgattttga
      841 gcataaagcg cattgggata taggtacagg tctaggtatt ttagattttg aaagagcagg
      901 gaaaataact gggtcaaggt ttactttata taggggatta ggagctagat tagaaagatc
      961 tttaatgaat ttctttttaa atactcatac agctaagcat ggctatacgg aagtcttacc
     1021 tccatttatg gccaatagaa atagttttat agggactggt caacttccta aatttgaaga
     1081 agatatgttt aaaatagaag gattagagta tttcttaata ccaacagctg aagttcctgt
     1141 tactaatata catgctaatg aaatattaga tgttgcagag cttccaatta aatactgtgc
     1201 atatactcct tgttttagat ctgaagcagg ttcagcagga agagatacaa gaggtttagt
     1261 tagacaacat caatttaata aagttgaatt agttaaattt gttaaaccag aagattctta
     1321 taatgaatta gaatcattaa ctcatgatgc tgaaactatg ttacaaatgt taggtcttcc
     1381 atatagagtt gtaaaaatat gtacaggaga tttgggattt acagctgcat ttaaatatga
     1441 tttagaagtt tggatgccaa gttacaatag atatgtagaa atatcttctt gttcaaattt
     1501 tgaagatttc caagcaagaa gagcaggaat aagatttaaa agagataaaa aatcaaaagc
     1561 agaatatgtt catacattaa atggttctgg attagcaata ggaagatgtt tggctgctat
     1621 attagaaaat tatcagcagg cagatggttc tgtagtagtt ccagaagaat taagacctta
     1681 tatgggtgtt gatgttataa aataaattta tattaattgt atatatagag tatgctttga
     1741 tgaagagaat atattatgag taaacaataa ataaaaaaac tatgaaatta ttataaatat
     1801 taatttc
//
LOCUS       DAEOSP010000328         1787 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.332, whole genome shotgun sequence.
ACCESSION   DAEOSP010000328 DAEOSP010000000
VERSION     DAEOSP010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.332"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            32..601
                     /gene="mocA"
                     /locus_tag="KRH54_003530"
     CDS             32..601
                     /gene="mocA"
                     /locus_tag="KRH54_003530"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBG8581158.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            598..1617
                     /locus_tag="KRH54_003531"
     CDS             598..1617
                     /locus_tag="KRH54_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBG8581159.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
BASE COUNT          708 a          164 c          345 g          570 t
ORIGIN      
        1 caatatatta taaataccaa ggatgatata tatgataaat gcagtgataa tggcatcagg
       61 ctttgccaga agaatgggag caaataaact tttattaaat tataaaggct gttctataat
      121 agaacatgta tttagagaag ttagcaagat tgattttcat caagttgtag tagtaagtca
      181 atatgaagag gttttgttat tgtgtaataa atatggtttt gaatatatag ataataaaaa
      241 tgcaaatata ggtcaaagtg aatctataaa attaggagtt ttaaatagct caaaatgtga
      301 tggttatatg ttctttgtag gagaccagcc atttattgat aacttatata tagataaaat
      361 tataaatact tttaaattag atagagattt tatagtaatt ccaagataca aagatatgtg
      421 tggaaatcca gtcatattcc catatagcaa gaaagaagaa ttgttggcac ttaaagaaga
      481 tgaaaaaggt aaaaccattt taaaaaattc atcaaaaata aagtatgtag aagttcctaa
      541 agaaatgtta tttgatatcg atacaaaaat ggactatgag agaattgggg gtaaattatg
      601 aagctaataa aaactataga tgctgttgga caagttttat gtcacgacat aactcaaata
      661 atacctggag aatttaaagg aagaaaattt aaaaaaggcc acatagtaaa agaagaagat
      721 attccagtgt tattatctct tggtaaagat aatttgtatg tatgggaaaa gtcagaagga
      781 atggttcatg aaaatgaagg cgcattgttt ttaaaagaac taactgctgg agaaaattta
      841 gattttagtg aaataaaaga agggaaaata gattttatag cagcatgtga tggtctatta
      901 aagatagatg ttgatgcact ttttgatttg aattgtgttg gagaaataat gatggctact
      961 cttcataata attttatagt aaataaaggt ttaaaagtag ctggaacaag agtgatacct
     1021 ttggttatag atgagaaaaa attagaagat gcaaaaaaag tagttggtaa tagaaaaata
     1081 gtaaatgtag taccttttaa acctaagaaa gtaggaattg ttactacagg aaatgaagtg
     1141 ttttatagca gaattgttga taaatttgga cccgtaatag aagaaaaagt aaaaggcttt
     1201 ggttgtgaag ttataggaca gactatatgc ccagatgata aagagattat aaagagtgca
     1261 ataaaagaat ttataagtca aggagcagaa cttatctgtt gtactggtgg gatgagtgta
     1321 gacccagatg atgttacacc aacagctata aaagaaactg gagctgattt agtgacatat
     1381 ggttcaccaa tattaccagg agctatgttt ttacttgctt attatggaga agttccaatt
     1441 atgggtatac caggatgcgc aatgtatcac aaaactacag tatttgatat agttttatct
     1501 agggttttaa ttgatgaaaa gcttgataag tatgatatag ctagatatgg tcatggtggc
     1561 ctttgtatga actgtgatgt atgtacttat cctgcatgta attttgctaa aatataaagt
     1621 tattaaaaaa gagtatttac aaattaagta ctctttttaa atatgaaaca aaacagctag
     1681 aatgatgttt taagaaaagc atatttattt agaaattata ataacttatt ttcaatgtgt
     1741 aaaagaatga ataaagttgc aatttgattt ctttataagt ataatat
//
LOCUS       DAEOSP010000329         1753 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.333, whole genome shotgun sequence.
ACCESSION   DAEOSP010000329 DAEOSP010000000
VERSION     DAEOSP010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.333"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            122..1450
                     /locus_tag="KRH54_003532"
     CDS             122..1450
                     /locus_tag="KRH54_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBG8581160.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          590 a          205 c          324 g          634 t
ORIGIN      
        1 aagctaagta gagtttattt tattttaata aataataaag ctatttttct atatattggc
       61 ataatatatg cttatttaac tatgaaatta aaattaaata ggattaatga ggaggaaaaa
      121 aatgggaagt aatgaactaa aaaagactct tggcgtatca gcagcattat caactgttgt
      181 tggttccgtt ataggagcgg gcgtattttt taaaccacaa gcagtctata cattaactgg
      241 aggagctcca ggtcttggta tattggcatg gttaatagct ggaatcataa ctataacagc
      301 tggtcttaca gctgcagagg tatcagttgc gataccaaaa actggtggaa tgatggtata
      361 tataaaagaa atttatggag aaaaattagg ttttttaact ggatggatgc aaattgtatt
      421 attttatcca gggatgatgg cagcattagg tgttatattt ggagaacaag catcagcact
      481 aattggtagt ccatcacttt tacttccaat agcaattgga attatagtaa tagttgcagg
      541 gcttaatatg ttaggttcaa aaactggagg agtcatacaa actgtttcta caatttgtaa
      601 gttaatacca cttattttaa taatggttgt tggatttatt aaaggtggtg gaaataatcc
      661 aatacttaca cctatggttg gtgaaggatt aagcctgggt agtgtactag gtcaagtttt
      721 aattgctata ttatttgctt ttgatggatg gatgaatgtt ggtactttag ctggagaaat
      781 gaagaatcct ggaaaagacc ttccaaaagc tataataggt gggttgtcag ttgttatggc
      841 tgtttatttt ataataaatt tagcatatct ttgggtatta ccagctaatg aattagcaaa
      901 ttatgcttct cctgcatcag cagttgctga agttatattt ggttcaatgg gtggaaaaat
      961 aatatctgta ggtatattaa tttcagtgtt tggagcatta aatggatttt tactaactgg
     1021 ttcaagggtg gcatatactt tagctacaga taagacttta ccaaagtatg gtatattttc
     1081 taaattaaat tctgctcaag ttccagcaaa tgcaatagca ttagtatctg ttattgcatc
     1141 tatatatgct ttgtcaggtc aatttaattt attaactgac ttagctgtat ttgctacttg
     1201 gatattctat gtattaactt ttataggggt tatgaaactt agaaagactc atccaaatat
     1261 accaagagag tataaagttc cattatatcc tattgttcct ataatagcta tagcaagtgg
     1321 tatatttgtt gtagttaatc aattgtgttt tgcaggtatg aaaactacta tgatatctat
     1381 aggaggtctt gtaataacag ctataggctt accagtgtat gcttatatga ctagaggagt
     1441 aaaaagataa accaagtagt ttacaaatgc ttcataatta aaataaaaga gactgtctca
     1501 aatagagata agtctctttt ttattgttaa aatggtaaag atatgtttga ttttacaaaa
     1561 aaagagcgtt ccatgaacta aaaagtttat tttaaggtat tcattttatt tttagttttt
     1621 tgattataaa attaaacatc taaaaaatta tttattttaa aatactttat tttaaagtgt
     1681 tttttatcaa actaagtggt atctgtacaa caaatattat acatagcttt tatgaaaatt
     1741 aaatctttct gtt
//
LOCUS       DAEOSP010000330         1744 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.334, whole genome shotgun sequence.
ACCESSION   DAEOSP010000330 DAEOSP010000000
VERSION     DAEOSP010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.334"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            99..1535
                     /gene="miaB"
                     /locus_tag="KRH54_003533"
     CDS             99..1535
                     /gene="miaB"
                     /locus_tag="KRH54_003533"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBG8581161.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT          736 a          175 c          310 g          523 t
ORIGIN      
        1 aacacaacta gaaaaataat aatatcttta aaagattttt cttatttgat ataattaagg
       61 ttatgtgtaa tataagaaat taactaggag gaatagacgt gagcaaaaga aaacaagtaa
      121 caatacctat agagaaaata caagaacaag ataaatatat agaacaaata cacagacaaa
      181 atgaagaata cttcaacaga actggaaaga gaaaactcgt gtttactcaa accttcgggt
      241 gtcaaatgaa tgaacatgat tcagaaaaat tatgttcaat gttagaagag atgggatacc
      301 aaatgtcaat gatggttgaa gaaagtgatt taataatata taacacctgt gcagttagag
      361 aaaatgcaga gctaaaagtg tatggtaact taggacaatt aaaacatcta aaaggaaaaa
      421 atccagatat gaaaatagct gtatgtggat gtatgatgca acaaccacat gttgtagagg
      481 aattaagaaa aaaatataaa catgtagacc ttatatttgg aacacataat ctatataaat
      541 ttccgcaatt acttacagaa tcaataaatt ctgacaaaat gttagtggat gtatgggatg
      601 tggatggaga agtaatagaa ggtcttagaa gtaatagaaa atttgaatta aaagcatttg
      661 taaatataat gtatggatgc aataatttct gcacatattg tatagtccca tacacaagag
      721 gaagagaaag aagtagaact cctgaagata taattaacga gataaaagag cttgttgcaa
      781 atggaacgaa agaaatcact ttacttggac aaaatgtaga ttcttatggt aagacacttg
      841 aaaatccagt ttcattctca gaacttttaa gaaaagtgaa tgatatagaa ggaattgaaa
      901 gggttcgatt tatgacatct catccaaaag atatatctga tgaagtaata tacgcaatta
      961 gagattgtga taaggtgtgt gagtttttac atctaccaat acaatgtgga agcagtagcc
     1021 ttttgaaaaa aatgaataga cattatacaa aggaatatta tttagaaata atagaaaaag
     1081 ctaaaaagga agttccaggt attgcttttt ctacagactt aatgattggt ttcccaggag
     1141 aaacagaaga agatttattg gacactttag atgtggttga aaaagttaga tatgattcag
     1201 cttttacatt tatttattct aaaagacagg gaactcctgc tgctaaaatg gaaaatcaaa
     1261 taccagaaga tataaagcat gatagattta atagggtgtt agaagctgta aataggattt
     1321 ctgcagaaat aaatgatgga tataaagata gaatagttga agttctagtt gaaggaagaa
     1381 gtaaaaataa tgaaaataag tttgctggta gaactagaca aaataaactc gtaaatttcg
     1441 aaggtggaaa tgatgattta attggtaaat tagttatggt aaaaattact gaacctagaa
     1501 ctttttcatt aaatgggata ttagtaaata attaatattg tacaaaataa taaaattatg
     1561 taaactaatc aaaatagatg tactatagaa caaattatat taaaaatttt tattttaagc
     1621 gaatttgtca tatgttatgc taatgtaagt ttttgctatt aataaaaata aatttgatat
     1681 aagatataat ttcattgtac atctattttt gttttattat attttataaa agcttaccta
     1741 gttg
//
LOCUS       DAEOSP010000331         1694 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.335, whole genome shotgun sequence.
ACCESSION   DAEOSP010000331 DAEOSP010000000
VERSION     DAEOSP010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.335"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(17..1354)
                     /locus_tag="KRH54_003534"
     CDS             complement(17..1354)
                     /locus_tag="KRH54_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG8581162.1"
                     /translation="MGTYSLISTSINFILILINIFIAFFLYTLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
BASE COUNT          674 a          203 c          209 g          608 t
ORIGIN      
        1 ataatctaat tttagactaa aataatctaa tataaagaaa taaagaaaat tcactctcag
       61 tataagaaat atccaatttt ccattgtact tattaacaat ttctctaata ttctcaatac
      121 catatccgtg agaattacta tcttttttat ctgaaataaa tagttcttgt tttctattga
      181 taggatttac tttgttgttt gtcaataaat agctaaaata ctccttctca caacgtgcct
      241 ttaataccac ttttctattt gacaaatcag gaattttcaa agatgcttct atagcgttat
      301 ctaaagaatt tgaaaaaata gtacataaat ctataggttc tatacccaag tgattgtcta
      361 tatctatatt aaagtgcaca tcaacaccat tttcttccaa acttgtatga cgaagattta
      421 aaattgagtt aagagcttgg tttttgcaaa attgtttgcc actaagagta cctataccta
      481 tttttaactg attaaaatac tttttagctc cagcaatatc ttcaatttct aaatacgatt
      541 caatcatttg aaagtgatta ttcatatcat gtcgcatttt gcgtatttga atttgttgat
      601 tttctagtaa gttatagtat ttttctttta ggtgatttgc ttgtttttct atcttcatct
      661 ttacattttc aatcaatggc tgtaaaagaa gtatcacacc gatatttgcc agaattgctg
      721 acaagatgat tagataagtg ccataaggta agttgcctaa gataatattc ttattactac
      781 ccatagggat taaattagta ggtggcagaa taatagcaat taatattgat aaaaatgaag
      841 atatacatac catatcaata aaaatccata ttttatttga taagtattgt tttatattga
      901 ccaatctatt ctttaggaaa atatgtatta aaaaccaaaa taataattta attagagcag
      961 aaattatctg gactattgaa agtattaaaa tatactcttt tgagtgtata agttcctcat
     1021 atatagggtt gtttataaaa agaaaattaa gcccaattat aataggataa agtatcatga
     1081 caactgatat tataggaatt aatttgcttt tgctacatat tataagtagt ataaaatatc
     1141 cgaatagtgt aaacaaaata tttacggggt cggaaagcca tattatcatt tgactaataa
     1201 tagagtagcc caaaatcaat atgattttga aaatccaatg attcctaaag tctataaaat
     1261 aggaagtcaa cgtataaaga aaaaaggcta tgaaaatatt gattaatatt agaataaaat
     1321 ttatgcttgt agagattaaa gaataagtgc ccatttaata tacctttcta ttaattaaga
     1381 ttctggaaac taagttttaa aaaattaaat cttaaaaatt aaaattctgg aaattagatt
     1441 tcaaagaatt gattttgaaa aataaaattc tagaaattaa gcttcaaaaa attgagtttt
     1501 aaaagattga aattttaaaa attaagtttc aaaaaattaa atttgaaaaa aactctaaaa
     1561 actaagcttc aaaaaattga attttgaaag attgaaattt taaaaattaa gtttcaaaaa
     1621 attaaatttg aaaaaaaact ctaaaaacta agcttcaaaa aattgaattt tgaaagattg
     1681 aaattctaga aact
//
LOCUS       DAEOSP010000332         1670 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.336, whole genome shotgun sequence.
ACCESSION   DAEOSP010000332 DAEOSP010000000
VERSION     DAEOSP010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.336"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            129..1601
                     /locus_tag="KRH54_003535"
     CDS             129..1601
                     /locus_tag="KRH54_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBG8581163.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAYKTAYKTAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPT
                     ETSARLKNVSKVYIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSI
                     KKVNTVMLTNAYKGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASM
                     STTLVNNTKSTRLGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGP
                     MVLVNDGSNKSILKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTI
                     KDGMYKVGKDISAGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKN
                     GQYIKIEDSTMTLAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRN
                     DSLGNAEGIVTNDTFSGRRYITVEEDQYLILNDCYLIENE"
BASE COUNT          686 a          171 c          278 g          535 t
ORIGIN      
        1 tgtattaata tttatttgta atatacttaa tataatttgc aattttacat aagtttgtta
       61 agtacataac taatttaaga atatgtattt atatattaaa aatttattat gagggggaca
      121 attttattat gaaattctat aaaagaatat taacattgac tttaagtatt tcttttgtat
      181 ttgcaaatat tcaattagta gatgcaatat catctgtaga aaaaatacaa ggaaaaaata
      241 agtatgaaat agcaggtaaa atagcagata aaaatgcata taaaactgca tataaaactg
      301 catataaaac tgcatataaa actgctatat taattaatac tagtaactct attgcagatg
      361 gattaagtgc aagtggacta gcaggagctt taaatgcccc tatactatta actgaaaaaa
      421 atactatacc aactgagaca tctgcaagat taaaaaatgt atcaaaagtt tatataattg
      481 gaggaacata ttctattagt acatctgtcg aaaattcatt aaaatctaag aaaatgaaag
      541 ttgtaagaat taagggaaat gacagaataa aaacaagtta taatgttgca aaagaaataa
      601 attcaataaa aaaagtcaat actgttatgc taaccaatgc atataaagga gaagcagatg
      661 ctataagtat tgcatctgta gcagcaagag ataaagcacc aataattctt actaatggac
      721 aaagtatacc tttttcaact agtggtttaa agtcttatgt aataggagga acagcttcaa
      781 tgagtaccac tttagtaaac aatactaaat ctactagact aggggggagt actcgttttg
      841 aaactaataa agcaataata aataagtttt ataaggatgc tagagagttt tatatagctg
      901 gagcttatga attaacaaat gcacttgtag gatcttcttt atcaaaacat ggtccaatgg
      961 ttttagttaa cgatggtagc aataaaagca ttttaaaaaa tgctaagaag ataacttcta
     1021 taggatatat agattcaaat atagtacaac agtgtttaaa tataacaaat ggaattggag
     1081 atattaatac tggggttgta aaaaatgtta aacctactac aaagactata aaagatggaa
     1141 tgtataaggt tggaaaagat atctcagcag gtgaatactt aataacttca aactcaggaa
     1201 gttatgcttc ttattatgaa gttacaagtg atagtacagg taatgctgat agtatacttt
     1261 caaatgatat ttttagtggg actagatata tcaccttaaa gaatggtcaa tatattaaaa
     1321 ttgaggattc aacaatgaca ttagcaaagt atgctaaagc ccagaaggcc aagaatggta
     1381 agtttggaaa tggtatgtat aaaattggat tagaaattcc tgctggagaa tatattataa
     1441 tgtcaaattc ttctgatgca tattatgaag ttagaaatga tagcttaggt aatgcagagg
     1501 gaatagttac taatgatact ttttcaggaa gaagatatat aacggttgag gaagatcaat
     1561 atttaatatt aaatgattgt tatttaattg aaaatgaata gatgtaattg tatttaatag
     1621 taaatcatat attttattta aggaaaggtg ttttaagttt taatttaaaa
//
LOCUS       DAEOSP010000333         1609 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.337, whole genome shotgun sequence.
ACCESSION   DAEOSP010000333 DAEOSP010000000
VERSION     DAEOSP010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.337"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..1083
                     /locus_tag="KRH54_003536"
     CDS             <1..1083
                     /locus_tag="KRH54_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBG8581164.1"
                     /translation="YVVDKWKSVFISLLYWMLLIGFDSIGLSIVMTLNSIKNMNDLLE
                     DNLLKLELIMISKSLLIVLIPLLKFIRLKTKINKRDCIYLSIPLIANIIGVIVIFEFI
                     FKDKIIDSKESLIILTISTVFLLSNLSLVSIIGRIIKDNSLKIEYEITKKRMNMQYKY
                     YLNLQEYQLKIRKLYHDMNNHIICIQNVYGKNEFADKYIKDINKQIKECNTIFNTQNM
                     ILDVILSDKKSICDMNNIYFLVDINFLECNFIEMPDICSIFSNIIDNAIEACIKIKDT
                     SIQKKIKLRGTVINRLFVIKCENTKINEVVLKNNKVITSKKDSFLHGIGISSVKSSVE
                     KYNGNVEIYSDKNKFTITIYIPLTRN"
BASE COUNT          696 a          109 c          239 g          565 t
ORIGIN      
        1 tatgtagtgg ataaatggaa aagtgtattt ataagtttgc tatattggat gttattaatt
       61 ggatttgata gtattggatt aagtatagta atgacactta attctataaa aaatatgaat
      121 gatttattag aggataattt gttgaagtta gagttaataa tgatatctaa atcattacta
      181 atagtattaa taccattgct taagtttatc agattaaaaa caaaaataaa caagagggat
      241 tgtatatatt tatctattcc attaatagca aacattattg gtgtaatagt aatatttgaa
      301 tttattttta aagataagat tatcgattct aaagagagtt taataatact tacaatatca
      361 actgtatttt tactatcaaa tctttcatta gtaagcataa taggaagaat aataaaagat
      421 aatagtttaa agatagaata tgaaattaca aaaaaaagaa tgaatatgca atataaatat
      481 tatttaaatt tacaggaata tcagttgaaa ataagaaaac tatatcatga tatgaataat
      541 catatcattt gtattcagaa tgtttatggg aaaaatgagt ttgcagataa gtatattaaa
      601 gatataaata agcaaataaa agaatgtaat actatattta atactcagaa tatgattcta
      661 gatgttattt taagtgataa aaaatctatt tgtgatatga ataatatata ttttctagta
      721 gatattaatt ttttagaatg taattttata gagatgccag atatatgtag tatattttca
      781 aatataatag ataatgcaat agaagcttgt attaagatta aggacacaag tatacaaaaa
      841 aagattaaat taagaggaac tgttataaat aggttatttg taataaaatg tgaaaataca
      901 aagataaatg aagttgtatt aaaaaataat aaggttatta caagtaagaa agattccttt
      961 ttacatggaa taggaataag tagtgtaaaa agttcagtag aaaaatataa tggaaatgtg
     1021 gaaatatact cagataaaaa caagtttaca attactattt atataccatt aactaggaac
     1081 taattctagt tggggctcta aattgcccag ttggagcagt agtattgaaa gataaaatag
     1141 aagatgctat ctttaaggca taggatattt ggaggtgata aaaatggttg aatgctatgc
     1201 taataagata acatcctttt tagtactcaa taaaataata gaacaaaaaa tgtgatttat
     1261 atttgtttga atttaaaatg ttgataggtt ttatagttat attatttgca tgatatatct
     1321 taaataaatt tagaaaaata gagttatttt tatctgcaaa tgcgacctta gtatggtaac
     1381 attattgacc aaaagttcta tatagtataa aaaaataaaa tgtaaatttg ataaacttta
     1441 taactaggaa cataaaaaca acaattaaat aactaattag atagtaaagt tgatattagt
     1501 aataaatatt aaagaaaaat attttactaa tttgaggagg acttagttgt gatagattct
     1561 gaagaaatga tttttaatgt agaagattta attatttata gaaaaaatc
//
LOCUS       DAEOSP010000334         1596 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.338, whole genome shotgun sequence.
ACCESSION   DAEOSP010000334 DAEOSP010000000
VERSION     DAEOSP010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.338"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            260..1531
                     /gene="serS"
                     /locus_tag="KRH54_003537"
     CDS             260..1531
                     /gene="serS"
                     /locus_tag="KRH54_003537"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBG8581165.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
BASE COUNT          665 a          167 c          291 g          473 t
ORIGIN      
        1 agtcaaaata gataattgat tagcaaaata gatataatta taatgaagtt aaatctagca
       61 atattctcct aaaattaaat ttcatttatt taattaaata taatttgaca atgggcaaaa
      121 aagtgagtaa aatataaata aaataaaaat aacaggttcg gataaagaaa aaggtttctt
      181 aacctcaagc ataaagtata ttatctaagt tatttgagat taagagataa atccaatctt
      241 aagtactgag gaggaaaaaa tgcttgatat aaaaagaata agagaaaatt tagattgtat
      301 aaaaaaagca atggaaagaa ggggcgaaaa agactttaac cttgatgaag tagttaagtt
      361 agatgatgaa agaagaaaga ttcttcaaga agttgaagtt atgaaaaatg aattaaatac
      421 agcatctaaa aatataccaa atcttataaa agaaggtaaa gatgtagaaa atgaaaagat
      481 taaactaaaa gagttatctg ataaaattaa ggtaatagac caaaatctta aagaagtaga
      541 agataagatg gaatacctat taatgagaat accaaatgta ccacatccag aagtaccaca
      601 aggtgaaact gatgaagata atgttgaagt aagaacttgg ggaaagacta ctacctttga
      661 ttttgaatct aaggctcatt gggaaattgg aacagaatta ggtatacttg attttgaaac
      721 agctagtaaa ataactggtt caagatttac tctttacaaa ggtttaggtg ctagattaga
      781 gagagcatta ttaaattttt acttagacac gaatacaaaa gtaaatggat atacagaagt
      841 tattccacca tttatggcaa atagaaatag ttttttaggt acaggacagt tacctaagtt
      901 tgaagaagat atgtttaaaa tagaaggtct ggactatttt atgattccaa catcagaagt
      961 tcctttaact aatatacatg ctaatgaaat acttaaattt gaacaattac cgataaatta
     1021 tacagcttat actccatgtt ttaggtcaga agctggttct gctggaagag atacaagagg
     1081 tttagtaaga cagcatcagt ttaacaaagt agaaatggtt aaaatagttg caccagaaga
     1141 atcttacaat gagttagaaa agcttactaa caatgcggaa actatgctac aattattgaa
     1201 tctgccttat agggtagtaa agatatgtac aggtgattta ggatttacgg cttcttttaa
     1261 atatgatgta gaagtttgga tgccaagtta caataggtat gttgagattt catcttgttc
     1321 aaactgcgaa gatttccaag ctagaagagc aggaataaga tttaaaagag ataaagattc
     1381 taaagctgaa tatgtacaca cattaaatgg ctctgggtta gctattggaa gaagtgttgc
     1441 agctatatta gaaaattatc aacaagaaga tggttctgtg gttgttcctg aagttttaag
     1501 accatatatg ggagtttctg ttattaaata agagttaaat tatgtatttt taagcaatac
     1561 aataatatag aggtaataaa aaataattaa tataaa
//
LOCUS       DAEOSP010000335         1584 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.339, whole genome shotgun sequence.
ACCESSION   DAEOSP010000335 DAEOSP010000000
VERSION     DAEOSP010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.339"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..698)
                     /locus_tag="KRH54_003538"
     CDS             complement(<1..698)
                     /locus_tag="KRH54_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG8581166.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLE"
     gene            823..1557
                     /locus_tag="KRH54_003539"
     CDS             823..1557
                     /locus_tag="KRH54_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBG8581167.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
BASE COUNT          535 a          235 c          225 g          589 t
ORIGIN      
        1 tcttctaaag ttaatccttt tgaataaatt tcctcggcag catccttacc aacatatcta
       61 atatgccatg gttcatatgc tgtacctgtt atatcttctt taccttcagg atatcttatt
      121 ataaatccaa acttgtgagc attttctgcc aaccatttag cttcttttgt acttccaaca
      181 aaccatcttt ctggatttgt taaatcaata caaagtccag tttggtgctc actgtaacca
      241 ggctttgcta catatgcatc tgccttttct cgtccctgag attttaccct tcttgtataa
      301 gtatccagtt gagtatcata agatctgtat gctgaagacc ctaaaaactg tattccttct
      361 gaattagctt gcttaactaa atcttcaact gcctttgcag gttctcctgc catttgtttt
      421 tcttcctctg ttgcttcttc aacaaaaggt atgtttacct tagtaatatt ttcaggagta
      481 tagtttttgc taattccatt tgtcttgttt accaataata tatttgatat actatttgag
      541 tgatttttat tttgagaatt tgcctcaaca tcttgttctg aatcatgttg agcagctact
      601 attggatctt tatctttctt aagtaatcta tctttagata taaatacact tataaatact
      661 actaaaataa tggctcctat taatttaaat tttttcataa ttaaatcttc tcctttttgt
      721 tatgtttgct ataatgttaa ttttagagta taagacttaa gtattttata agtaaatctt
      781 aaataaatct taaatttaat atttttaaag gatggtgata aaatgtctat acaaaaactt
      841 ctaagtaaag ctcgtaaggc tatacaagat tttgatatga tacaagaaaa tgataaaatt
      901 gcggttggac tttctggtgg aaaagatagt ttaactttac ttcatatact taaaagttat
      961 caaagatttt ctccacaaaa ctttgaattg atagccataa cattaaatac tggaggtgtt
     1021 gataattctc cactagataa attgtgtaaa gaaataaatg ttccttttta tgaatttcaa
     1081 actgacataa aagaaattgt atttgatata agacaagaaa agaatccttg tgcattatgt
     1141 gctaatttaa gaagaggtgc tttgaacgat aatgctaaaa aacttggatg taataaagta
     1201 gctcttggtc atcataaaga tgatgcaatt gagacatttt taatgtctat gttttatgag
     1261 ggtagagtaa attgtttttc cccaaagaca tacctagaca gacaggattt aacagttata
     1321 agacctatgg tttacataga agagtatatg actaagaaaa tttcaaaaga ttctaactat
     1381 cctattatca caaatccctg ccctgcaaat ggtcatacta ggagagagta cataaaaaat
     1441 cttattgcta atttaaataa agagatgcct gattttaaaa gaaatgtatt tggtgcttta
     1501 aataattctg agaaattgtt tatatgggat aaagagaaga taaaaaattt taaataataa
     1561 ttaaaatttt taaataaaaa agca
//
LOCUS       DAEOSP010000336         1570 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.340, whole genome shotgun sequence.
ACCESSION   DAEOSP010000336 DAEOSP010000000
VERSION     DAEOSP010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.340"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(111..445)
                     /gene="rnpB"
                     /locus_tag="KRH54_003540"
     ncRNA           complement(111..445)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KRH54_003540"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            577..1254
                     /gene="srtB"
                     /locus_tag="KRH54_003541"
     CDS             577..1254
                     /gene="srtB"
                     /locus_tag="KRH54_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBG8581168.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSNINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(1414..1488)
                     /locus_tag="KRH54_003542"
     tRNA            complement(1414..1488)
                     /locus_tag="KRH54_003542"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1454..1456),aa:Thr,seq:tgt)
BASE COUNT          605 a          246 c          195 g          524 t
ORIGIN      
        1 aatatctgta ctaacttata ttacaatatt cctcaaaatt ttaaaagtag attagacaaa
       61 tttacaaata aaaaaacatc agtttaaact gatgtttttg actatcgaaa taattaagca
      121 agtctataag cggagttctg tattagataa tcatctttct aggcctattg tcaccaatag
      181 gctcaagcga cctacctacc ggagagtacg agcagcactc tttattccta tcttggtctt
      241 gctccaggtg gggtttacat agccaactag tcgcctagct gctggtgagc tcttacctcg
      301 cctttccatc cttaccacaa tagtggcggt ttatttctgt tgcactatcc ttaggatcgc
      361 tcccactagg cgttacctag caccctgctc tatggagctc cgactttcct catgtaagat
      421 tacatgcgat tatctggctt acttaatatt tattttactt agttatgtta acacaaattc
      481 ttttacatgt caaagtaata ttatactaaa aatttaaatt tattcaaaaa tagtgtaata
      541 tagatatata aactacattt aactcgaggt gatatattga aaaaattata tagaatagta
      601 attaacatta tactagtatt agtaatactt tacagtggat ttaatatata ctcaaaactt
      661 accaaataca atcatgatac taaaatatcc tcagaacttc aaaaaaaaga atataaaaaa
      721 gaggatttat caaatattaa cagtgatttt aaattttggc tttctgttga gaatacaaat
      781 ataaattatc cagttgtaca atctaaagat aattcttact atcttgacaa agatttttat
      841 aaaaaagatt ctatttctgg tacactgttt atggattata gaaataaatc tattgatgac
      901 aaaaatatca taatatatgg acataatatg aaaaataaaa ctatgttcaa caacctaaat
      961 aagtttaaag atgctgactt ctttaaaaaa aataataaaa taaaaataac cttgaatgga
     1021 aaagaatttt tatatgatgt tttttctgct tatattgtag aatctgatta tgattatcta
     1081 aaaacaaatt ttaacaatga atctgattat caaaattaca taaatgatat tacttctaaa
     1141 tcattgtata aatctcctat taaggtaaat agcaatgata aaatagttac actttctaca
     1201 tgtacctatg aatttgatga tgccagaatg gtgattcatg gtagattgat ttaaaactca
     1261 cagacacagt ttaaaacaat aattttaaac tgtgtcatct tttataccta taatttgtaa
     1321 attagaccac taaaagtatt taatacaaaa taacaaatac atcaaacaat aaaaaacaat
     1381 aaaaaaagac tatgctaaaa acatagtctt tactggagct ggtgatagga atcgaaccta
     1441 caacctgctg attacaagtc agctgctcta ccgttgagcc acaccagcac acctatttta
     1501 ttgtaaatta gcaaaactaa tttttaaaag ttaacactct aaaaactaca catatatctt
     1561 aatgaatgaa
//
LOCUS       DAEOSP010000337         1567 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.341, whole genome shotgun sequence.
ACCESSION   DAEOSP010000337 DAEOSP010000000
VERSION     DAEOSP010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.341"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            70..492
                     /locus_tag="KRH54_003543"
     CDS             70..492
                     /locus_tag="KRH54_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBG8581169.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            1020..1388
                     /locus_tag="KRH54_003544"
     CDS             1020..1388
                     /locus_tag="KRH54_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8581170.1"
                     /translation="MKKIPKSELLVMNFIWDKGIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
BASE COUNT          673 a          154 c          193 g          547 t
ORIGIN      
        1 catattatac tattttaact taaaaattaa cttataaata ttaaatttat atacagaagg
       61 gaaaacgata tgaatacatt taaaactgaa gttttattaa aatattctgc aaaagacata
      121 tttaatatat ttacaaaatc tgcaagacta aattttccta attttaatga gaaaaaagca
      181 gtaggaacat ttgtacagca aaaaaataat aaaagattta aggttgaaat aactaatttc
      241 gaaaaaaata gagtatatga gataaaaaca agcaataaaa gagaaagtac tattactaga
      301 tatgaattag tacatattga cttagaaaat acaaaactta tttttattga aagtgagagc
      361 aagaggaata tatttggaaa aatcaattca gctcttgtaa gagtattgtt tggaaaaagg
      421 caaccagaag aatttaataa atttataaaa aaactagaaa tggaattgga gaataatagt
      481 gttaatttat agatataagt tagattatat atttaaaatc tatctaaaaa catatttcta
      541 agtattttat gagtcctact attcgttaca ataattaatg aaaaaactat tgataaacat
      601 gtgacactat gactatatac cacattaaga gtatcattag caataataat tttatgtatt
      661 attgctttta gttaaaaaaa gagagaaaaa ttaataatct ctaatatccc ttaaactttc
      721 aataaagagg ataatcttta tctaataaga ttctcctctt ctaatttaga aaagatatta
      781 atttattcac tatatagtat ttttgtacaa atcaataaaa aatatatgtc atattcttat
      841 ctctaataga aaatatattc ccctcttcca taaaactctc aatatctttt ttatctataa
      901 atctatacta cttagttctc aagcataaaa taatataaag attatttata catgatgata
      961 tattatattg actattagac atttgtctaa tataatcaag ttaaaggtga gtgattatat
     1021 tgaaaaaaat accaaaatcc gagttgttag ttatgaattt catatgggat aaaggcatag
     1081 ataaaatacc agccagagaa gtagctaatt atatggaaat ttctcatgga tggacaaaag
     1141 gtactactgg taaagtactt tctagattaa gtgataaagg ttttttagaa tttgaaaaag
     1201 atggtagaaa tactttatac actattaaag ttaaacatga ggaatatcta aaatttgaaa
     1261 caaaggattt tttagagctt atacataaaa attctattca tagctttata tcatcattag
     1321 gttgtggaaa tgaaatatca gatgatgaca taaaagaact tgaacaatgg ctaaaaaaaa
     1381 gaaaatagga gacgtatttt atggaattat ttatagaagt tattaaattt aatatttaca
     1441 taagtccact aattcttttt ttcattttat ttactaaaaa gataagcaaa tatactttta
     1501 agtttaatta ttttatatgt cttttaatag tagtcagaat gatgtttata tctaaaataa
     1561 atttaaa
//
LOCUS       DAEOSP010000338         1555 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.342, whole genome shotgun sequence.
ACCESSION   DAEOSP010000338 DAEOSP010000000
VERSION     DAEOSP010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.342"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(378..1085)
                     /locus_tag="KRH54_003545"
     CDS             complement(378..1085)
                     /locus_tag="KRH54_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBG8581171.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT          503 a          267 c          137 g          648 t
ORIGIN      
        1 ttaaagtata ataaaatata taaaaaaata tataaaatgg tactataaaa tttttaatct
       61 tcatcaatac cttttttgct ttcattgtta aaactatttt ttctattagt ttatttctta
      121 taaaacaaac atttagtatt aaaatatata tcacacaata gtatttgcaa tttaacttta
      181 aaaaataaaa atacccaact gacctaagca actcgattgg gtataaataa tctaaaaaat
      241 catttttgta taaactcttt atttaactta catttttatt taccttctac tttaattcaa
      301 cttatatttc tatgcttctt atactctcat tcaacttata tttctatgct tcttatactt
      361 ttataaattc atcacaatta tttagttctc gctatcaaag aaattgtatt aaaatacttt
      421 ccattctcta cttccatcat ttttttgtca tttatagcat atgaattatc acaatttatc
      481 caatccttcc aagcttcatc atgacaattt aaagcttcac atttctctac aatcacagac
      541 tccgattcag accaaagttt attccaccat gtaattgaat ggaagttcat attatcttgc
      601 caaaatggaa tgagttcatc tggtatacaa tccacgaaat cttcttttaa tccaggcatt
      661 gccatagcaa gtattccccc ctcttttaca agaggtgata tatgattgtc taaaaatcct
      721 tctttgtttc caaaatagtg ataagaatcg atacttatca ccgcatcaaa gaaaccttct
      781 gcataaggta gttcatgtgc ctctgcctgt attggaaata ttttatcatc caatttaaac
      841 tctttaaatc ttttatagtt ttcagttggt tctatccata aatctgttgc aaatacagta
      901 gcatcaaatt cctttgctaa aaatatcgaa gatatacctt taccacaacc taagtctaaa
      961 atacgcattc ccttttctag ttttatctta cttgttaatt cttccaatat ttttatacaa
     1021 ttaggtccca tcatattttt catgagaaag tccttatcgt atttattact ttttgtaaat
     1081 atcattctat ctttcctttc cctatcagtt attatttatt aaattttata cgtttgattt
     1141 tttatttttt atatgcttct tatattgagc aatctctttt ttattcattc tttaacagac
     1201 aattttaatg tgtaatttta tccactctgt gatagtaact ttaatgaata atcttatcta
     1261 ctgtgatagt tattttagtg agtaatttta tctactccac agcaatcgct ttaatgagta
     1321 atcttatcca ctctatgata gttattttag tgagtaattt tatctactcc acagtaatcg
     1381 ctttaatgag taatcttatc cactctataa tagtcacttt aatgagtaat cataattttt
     1441 atatcatttt agatacttac tctaataaga agtcattttc taatacttaa taagttttaa
     1501 ttacttccac tttaacctat atactaatta acttatttaa tttagtgcat atttg
//
LOCUS       DAEOSP010000339         1504 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.343, whole genome shotgun sequence.
ACCESSION   DAEOSP010000339 DAEOSP010000000
VERSION     DAEOSP010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.343"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..860)
                     /locus_tag="KRH54_003546"
     CDS             complement(<1..860)
                     /locus_tag="KRH54_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBG8581172.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNL"
     gene            complement(872..1381)
                     /locus_tag="KRH54_003547"
     CDS             complement(872..1381)
                     /locus_tag="KRH54_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBG8581173.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
BASE COUNT          504 a          239 c          144 g          617 t
ORIGIN      
        1 agattacctg tattattaaa taataatgaa gaaggaacaa atggaacttt tatatcattc
       61 tcatcaaatt tattttttac cttttccatt acatcaaaat atacatccca gtaatcatca
      121 gtttttaccc aaggtctagc aactatttgc atggtgttat ttcctataga gtttaaacct
      181 attataggct ttggatcatt taatattctt ttttcgtttg taaaaatttc atttaatata
      241 gacttaacta catctatatc actatcatat gaaatattat atgaaaaatc tattcttctt
      301 ttgttttgat gtgtgtaatt aattatatta ttacttgtca gttgaaaatt aggtataatt
      361 atagttttat tatctggcgt cttaagaaca gtgctaaata tttgtatgtc agaaactgta
      421 ccttctacac ctgaaccttg aatataatca cctatattaa atggcttaaa aaataaaata
      481 atcataccag agcctaaatt tgataatatt tctttaaaag ctaaacctat actaaatcca
      541 gcagctccta acacagctac aactgatgta gtttgtatcc ctataatacc taagcttatc
      601 aatgatataa tagataacat agatatatat attccatata caataaaatt atttacacct
      661 ttactcattc catatttgat tagaaacttt gaaaccatcc ttcttactat tttagatata
      721 taaataccta tcacaaatac aattattgca tatataatat ttggtatatt atcactaaaa
      781 ttatcgagca tattttctaa agttctctta gttatattgc tatttgttga ggcagtctta
      841 gccaatgata aaaaattcat tcaactcctc cttatgataa tattccgttg tcctttaact
      901 cttttactat agcttctata gtttcttctt ttattgcctc aattgtatga atatggctac
      961 attctgtcag aaccgataat tgtttaaatt catttgtctc aactttcttc ataaagttgt
     1021 ctatatccat tttagaataa atattaagtt ctgcagttat ctgaccatat gttggatgat
     1081 ttacgatgac atcttttata ataccaccta aatccacaat tatctttaat tcatcataaa
     1141 tttctttaga atccttatga tttttacact ttatttgttt tgtaaaatac ggttgattat
     1201 atattatata tccttgaggt gtagctatta tttcaaatcc tctagctctt ataagggcta
     1261 tatcttgaac tattacttgc ctacttacat ttaatagttc agctagtgtt ccacctttta
     1321 caggatgctt agattctttt aaaatatcta ttaatttact tcttctttca ttagaattca
     1381 tattacacct tctttccaaa taaaaacttt acctatacta tataatttta tattttatct
     1441 tattataaat caatattgac tttatattct atttaatctt aacttttata ataaataaaa
     1501 aaga
//
LOCUS       DAEOSP010000340         1471 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.344, whole genome shotgun sequence.
ACCESSION   DAEOSP010000340 DAEOSP010000000
VERSION     DAEOSP010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.344"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(73..510)
                     /locus_tag="KRH54_003548"
     CDS             complement(73..510)
                     /locus_tag="KRH54_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBG8581174.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            complement(767..916)
                     /locus_tag="KRH54_003549"
     CDS             complement(767..916)
                     /locus_tag="KRH54_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBG8581175.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(929..1159)
                     /locus_tag="KRH54_003550"
     CDS             complement(929..1159)
                     /locus_tag="KRH54_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBG8581176.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
BASE COUNT          495 a          184 c          167 g          625 t
ORIGIN      
        1 tattattatt aattattaaa taggtcaata aatttgcaat tagtgatatt aataaattac
       61 tttagatttt tattaaatgt ttagcttttc tttaatattt tttatataac gacgatttat
      121 aataactttt tcgttgtttt ttaaagtagc ttgcatgcga ttatgtgata ttacttttat
      181 actttttatg tagtccatat ttaaaatcat ggatttagat attcgtgaga aggatgtatt
      241 agctaataat tttgataatt tatatatttt taaatcaatt tcaaatacat catcttcata
      301 ataagcaaat gttttatttc caactgtctc aaagtataaa atatcacaga cattgagttt
      361 ataaatctct cctgattttt taccttttat atcaagtaac ggtgtactca ataatgaaat
      421 tattttttca atgttattat ctagagcaga acattttatg gttatttcaa tatcatcaca
      481 attaatacct tgttcgacat gtattttcat taatcatcac ctcattttat tactgtcatt
      541 agaatagcat tatatcttat tgagtccaac tagatgcgtt ctaaatttca aattaagcat
      601 agtgaaattc aaaaataaaa cagtgaaatt caaatttgag gtggtaaaat tcaaaaaaat
      661 actatggata tttggataaa ttatccaaat atcctagtat ttcacataga atatgtctaa
      721 ttattttaca aatagaccta ttttatgact tacatatatt gtcaaattac ttcttaaata
      781 tcaaaatatt tttggataat gtatcaatac tgtttgataa agtatttaat ttaccctcaa
      841 ttctaaccaa taagtacatg gatagagcta taggaaaacc aacattggtt attaacagtt
      901 gtaaactatc atccataaaa ttagccccct attctataac taagtcataa ggagtggtat
      961 ttgtttccac aaccttggct tcaacaacag aagctaggtc aaatcctcca accgaaaaaa
     1021 tatttttaga tactatcaaa tccatgacag attttatatt ttcttcagtt aaatcatcct
     1081 ttggattttc tatagaaaaa gaaacttgtt tatcctttac atttttaaag ctcatgacta
     1141 attttttctt aatttccata taattacctc cgatataata atttatattt taattgtttg
     1201 cttgttaata ttggtatttt aattgtcaat attaatgttt tgcttgttag tattaatatt
     1261 tcaattgtca atattaatgt tttgcttgtt agtattaata tttcaattgt caatattagt
     1321 gtcttgcttg ttagtattaa tatttcaatt gtcaatatta atgttttgct tgttagtatc
     1381 agtgtttcaa ttgtcaatat tagtgttttg cttgttagta ttaatatttc aattgtcaat
     1441 attagtgtct tgcttgttag tattagtatt t
//
LOCUS       DAEOSP010000341         1423 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.345, whole genome shotgun sequence.
ACCESSION   DAEOSP010000341 DAEOSP010000000
VERSION     DAEOSP010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.345"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(212..1150)
                     /gene="pdaA"
                     /locus_tag="KRH54_003551"
     CDS             complement(212..1150)
                     /gene="pdaA"
                     /locus_tag="KRH54_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBG8581177.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDDNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTT
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT          482 a          194 c          175 g          572 t
ORIGIN      
        1 ctaaatataa catctaaaat actgttatct atatttaata tcttgccaaa ttttttgatg
       61 ttttattgtc ctgtcccacc aacatatata agctatcaca agtttttacc tatactatca
      121 ccaaacaaaa attctaattt taatttatta ttttataacc tatattatat taactatatt
      181 agtttgtaga ctttagatgt aattataaat attaatactc caagagttca aattcatatc
      241 ctagttttct tgcatttgat ataaaatcat ttaaaatttc tgtactagtt ttggaaactg
      301 catgtagtaa caagatagaa ccatcatgta gattatccat tattttttgc gttgcttctt
      361 catgtgatgg ttgtttatca gtatcccagt ctctgtatgc aaaactccaa aatacagtct
      421 tgtatcctaa atttttagtc attgccaagc ttttttctga gtattttccc ataggaggtc
      481 taaaaaattt aaccatatct ttcccagtta catctttata taacttttca acatcatata
      541 attcgtcatt aaagtttttt agattgcttg tttttgttgg catagatgga tgggttttac
      601 tatgatttcc aactatatgt ccctcagaaa ccattctttt tactaaatct ttattttctt
      661 ttatatatgg agttgtaaca aaaaaaactg ctttaacctg gttttgtttt agagtgtcga
      721 gtatctttgt agtgtatcca ttttcataac cttcatcaaa tgtaagatat agtgtttttt
      781 gaccatcttt tgtaggtcca ttgtataggg catcatagtc agaaaattta aattcaatgt
      841 ccgtatttac ttctggtgtt ttatgcttat tgtttggtat ataaaaccaa tcaagagtag
      901 tgttatctaa agaagataca tctaatttat tatcatcatt attagtagtg gcttttgatg
      961 gtttatctgt tccgtctgaa actattacat tggaagtatc tttaccacta tctatttttt
     1021 ccttatcttc ttgtaactgt gtttccttat tttgattttc gttttgattg ttttgtgaat
     1081 ttgaacatcc aacaataaaa aaaatagaaa tagaaagtag tgttaaataa tgcaatactt
     1141 ttctaaacat tttgaagctc ctttctaaat taataataat tgcatataat aataattata
     1201 tgattaatta caattgcata tagtattatt ataattggaa attactatta ataatagtta
     1261 tatctttaaa aataaagtca caatagtatg acaaagggtg aataatcaat aaatttaaaa
     1321 aaaaatgaaa aaaatagttg accatattat gagaagatgt tattataata aatgtgctca
     1381 acaagaagta acaaaataac aagcacaaag aagttaattg aat
//
LOCUS       DAEOSP010000342         1403 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.347, whole genome shotgun sequence.
ACCESSION   DAEOSP010000342 DAEOSP010000000
VERSION     DAEOSP010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.347"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            20..418
                     /locus_tag="KRH54_003552"
     CDS             20..418
                     /locus_tag="KRH54_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBG8581178.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNVK
                     INLNIKGYKFLPYVKNSKASRDNLCFKINTNIDEPKKYLEQNEIQIERGIVECTGVFG
                     NIKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            406..1344
                     /locus_tag="KRH54_003553"
     CDS             406..1344
                     /locus_tag="KRH54_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBG8581179.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHINPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
BASE COUNT          508 a          173 c          192 g          530 t
ORIGIN      
        1 aaaatcttaa ggagttgcca tgataaaaaa aatagaacat gtggtcatta ctaataataa
       61 tatagaaaat tatatcaatt tttataaaaa atttggtttt tcatctaaag aaatgagcaa
      121 aaattataaa cttaatgcta gaaatgttaa gataaatctt aacataaaag gttataaatt
      181 tttaccttat gttaaaaatt caaaagcaag tagagataac ttatgtttta aaataaacac
      241 aaatattgat gagccaaaaa aatacttaga acaaaatgag attcaaattg agcgaggaat
      301 tgtagaatgt acaggtgtct ttggtaatat aaaatctatt tatttgaaaa aaactaatgg
      361 aaatttaatt gaattatcaa atcatagaat cacggagagt aaaccttgga aaatttaatc
      421 ctatcaataa atgttgttct tcctttgttt ctcacaatgt cacttggata tgttctaaaa
      481 aaattaggtc tatttgatac tcatacttta aataaaatga ataatgtctg cttcaagtct
      541 ttcttgccat tattattatt ttttaatata tacaatacag atttagatgg ttctcttaat
      601 tttaagctta tggctgtagc tgttataatt attttttcaa tcttcttaat cttagtcttt
      661 attattccta aaattgaaaa agataataag acaaggggtg cattaataca aggtgtattt
      721 cgtagcaatt tcgttatatt tggtattcct gtgacaattt cactatttgg cgataagagt
      781 attggagtca catctttatt aattgcagtt attgtaccta tgtttaatgt attatctgtt
      841 gtaattcttg aaatttttag aggtggcaaa gtaaatataa aacatatgat taaaagcgtc
      901 ttaacaaatc cactcataat cgcctctttt ataggattga caatgctaat tttaaatatt
      961 aatataccta catttttaca caaaactatt ggtgatattt ctaaaatagc tactccttta
     1021 tcacttataa ttctaggcgg ctcttttagt ttttctgcta taaaggggca tataaacccc
     1081 attatattgg gcgtatgtgg aaaacttata cttgtacctg ctatattttt accaattagc
     1141 atttttcttg gatttagaaa tgtagaatta gcttcactta tgataatgtt atctgctcca
     1201 actgcagttt cttcatttac aatggcacaa cagatggatt ctgacagtga attagctgga
     1261 caaattgtag tatttacttc tggattttct ataattacag tatttttaat aatatttatt
     1321 ttaaagcaga ataatcttat ttaatgatat ttgataacac aaattggtat tatgagaaac
     1381 atatataaaa ttgatttaac tgc
//
LOCUS       DAEOSP010000343         1386 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.348, whole genome shotgun sequence.
ACCESSION   DAEOSP010000343 DAEOSP010000000
VERSION     DAEOSP010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.348"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            75..320
                     /locus_tag="KRH54_003554"
     CDS             75..320
                     /locus_tag="KRH54_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBG8581180.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKLLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(538..807)
                     /locus_tag="KRH54_003555"
     CDS             complement(538..807)
                     /locus_tag="KRH54_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBG8581181.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
BASE COUNT          551 a          211 c          155 g          469 t
ORIGIN      
        1 aatattaact ataataaaaa aatatctggt aataggtata atataaaaag aatataccag
       61 aaagaaggtg aagtatggga gaactacaaa aaattccagg ggtagggaaa gcaacagaaa
      121 aatctttaat catgttagga tatacaacta ttaaattact taaagatgca aacccagcac
      181 aaatgtatga aaaggaatgt ttaatgcgtg gacagcacat agacagatgt cagttatatg
      241 tatacagatg tgcagtatat tttgcatcaa ctgaaaatcc agaaccagaa aaattaaagt
      301 ggtggtattg gaaagattaa aaaatcaaaa aagttagaca taaaaaagag ttgaatatcc
      361 gttttaaata ataaaaacta atactcaact cttttttagt cgtaaatttt atgtaaatct
      421 atttgtatgg ctaaatatac ccatcaataa atgtataaat ataccaatca acaaatgact
      481 aaacatacta cctaacaaag gattaaataa attacttaac aaagcattct aaaacaatta
      541 attagaaact gtttcttcaa gccattttct acattgctca atatttacca tagggtcaaa
      601 accttggtct tttcccttat ttaaaagcat atcacaaggg aaagtatcac attcaccaca
      661 atgattaagt tccttacttt cacagcaagt tttaacctta cattctccac cccaaaatgg
      721 tttttccata gttgggcaac cagtgcaatt tacttgttct ttttttgtac aactgtcaca
      781 acagacacca catcttgatt caaacatgtt caaacatcct ttccattatt taaaaatatt
      841 ataaaatgat tttagatgaa tacaaaagtg caaatagttg attttaggtc atctaaatgt
      901 taaacataaa attattagta ctggtaaatt tataaaaact attccaaaaa tctttttgaa
      961 tatttcaaaa cacaataaat tacttcaaat ccctttgaat atattttaca gcattataga
     1021 ttgctttaaa tctctttgaa tattctacaa gactatagat tagtttaaat ccctttgaat
     1081 atattttaca acattataga ttgttttaaa tctctttgaa tattctacaa gactatagat
     1141 tagtttaaat ccctttgaat atttcaaaac accatagatt actttaaatc cctttgaata
     1201 ttctataaca ctataaatta cttttaactt ttatataacc ataaagtttt tatctggcac
     1261 tttaaatgga gttttatatc aaatttgcct tccatcgaat aaccaaactt aatttaatta
     1321 cacattccaa gagtcaaata ttcattcaat atatttgata attctatgtt atagctataa
     1381 ttaaat
//
LOCUS       DAEOSP010000344         1383 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.349, whole genome shotgun sequence.
ACCESSION   DAEOSP010000344 DAEOSP010000000
VERSION     DAEOSP010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.349"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..598
                     /locus_tag="KRH54_003556"
     CDS             <1..598
                     /locus_tag="KRH54_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBG8581182.1"
                     /translation="KNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEVRSKNL
                     KHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVKFIIHP
                     YLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGSIPNQK
                     DYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            896..1234
                     /locus_tag="KRH54_003557"
     CDS             896..1234
                     /locus_tag="KRH54_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBG8581183.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
BASE COUNT          540 a          157 c          184 g          502 t
ORIGIN      
        1 taaaaataaa ttcaaaaatt acaaaccata tataaatgga tacaagaata tgaagagagc
       61 atctgtttta attccaatag tagaaataaa taacacacac tatatattat ttgaagttcg
      121 ctcaaaaaat ttgaagcatc aacctagtga aatttctttt ccaggtggta aaatagaatc
      181 aggtgagact ccatatgaag ctgttattag agaaacttgt gaggaacttg gaacttttag
      241 tgacaatata gaagtaatat caagtcttga cctccttata acaccagtaa agtttatcat
      301 acatccatac ttaggatata taaaaaatat taataattta aatataaaca aggatgaagt
      361 tgaccatact tttttggttc ctataaaata tcttttagag aatcctccaa atacttatag
      421 taatactgta aaagtttttc cagacgaaaa tctaccatat ggttcaattc caaaccaaaa
      481 agattataaa tttgaaagtg gaagttatcc tattctgttt tataaatata aagattatgt
      541 aatttgggga atgacagcaa aaatactaga aaacttttta aattttttat cagaataaca
      601 ctcataataa atcatcaaaa cttttacata agaaatttat aaaagtttct aaatttaaca
      661 attctaaaat atcaataact atattttgta gcactttttt attttattat tgtttgaata
      721 tttgatggtt ttcaatgcaa tttcaacata aatactatgt attagtgtat actgttgatg
      781 aattaaagta tgataatcta tttaataaag tatgaagtaa atgattttaa tgtgccttta
      841 tggtaaaatt actaataagt aataaaatta attaaaatat atggaggtgc ttataatgtt
      901 tgctgtagga tttgttgtta taattgcact tgcctttttc attttaagtg tactattatt
      961 taatggaaaa gccagtatgc tactagcagg ttataactct atgagtaaga aagaaaaagc
     1021 acgatacgat aaaaaaaaat tatgtagaag ttctagcatt gttactttta ttgtctctat
     1081 aatgctattt attatggcgt ttttgggata tagaatagag agtggtcaaa tggacgaaaa
     1141 aaaaatggtt cccttttcta taatatttat aatagtcata ctttccagtg tattttttaa
     1201 tatcctctac tctaacaaaa aatgtaaaaa ataaatagtt gtaagttgag cagttaatta
     1261 taaaagattg actgcttttt catgtatatt gactactctt attaaatatt ttaacattta
     1321 aaaatctatg taaaaataat gaatatagta caaacttcta ataatgattt tacctttgtg
     1381 gtt
//
LOCUS       DAEOSP010000345         1339 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.350, whole genome shotgun sequence.
ACCESSION   DAEOSP010000345 DAEOSP010000000
VERSION     DAEOSP010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.350"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(194..505)
                     /locus_tag="KRH54_003558"
     CDS             complement(194..505)
                     /locus_tag="KRH54_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581184.1"
                     /translation="MKKIIKSFTFWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(561..1154)
                     /locus_tag="KRH54_003559"
     CDS             complement(561..1154)
                     /locus_tag="KRH54_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBG8581185.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT          474 a          195 c          170 g          500 t
ORIGIN      
        1 aagaaatata gaaaattaat ttaaagaaat acaatacatt aatttttaag taacattaaa
       61 ttaatgtaag taaaataatg ttcatatgaa gatttaaagg tgcattttct gaagctaagt
      121 atatgttgta gagtgttagg tctctctatc ctgtgaagtt taatagctca agaaaatgca
      181 ctatttatga tgattatttt gttttatttc caattttgga aataactatt ctaattaaat
      241 ctaaaattat accataaata agaaaagaaa gtatagatgc tatgtaccaa taaatcgaaa
      301 ttgaccctgt aattgttcta catgggattt gcatacctga atccataaaa acaaaaaaac
      361 tatcaactct ggaaataata gacaaaatag gatttaaacc tattagaaca atactttttg
      421 agtcttgacc aatttgatgc atataaatct caaataatgc aattaaaaaa aaccaaaatg
      481 taaaagattt aataattttt ttcatcaaaa tacctcacat aatataaatt ctgtttgcac
      541 tatttgctta ctacaactat ttatctccat tcagtcacat cacttaattg taatctaggt
      601 cttcttgatg gttcttcatc aggataaccc aaagatattg ctgccactaa ttgactatct
      661 gtatttaacc attcacataa ttcacgatat gcaaagtata catcacaaat ccaaagacta
      721 cctagaccta attccacagc tgtaagcgac atattctgaa ttgcagctcc aattgattgc
      781 atgtttgcca tttcatagaa tcgttcttca ggggatagct tttctaaagg tgatttacct
      841 aatatattta aaataaaaat tgttactgga gcttgtttca tgatttcaac tgtgtaattt
      901 gcaccagcga tatgttgacg acttcctgga agaagcccat tatcattaat ttcattttga
      961 atacctttgc tcatggcttt aagcatagat tctttttcct tttcagtgat gacaacaaaa
     1021 cgccatggtt gcctgttttt agatgaaggt gcatttattc cagcttcaat tattttctct
     1081 atagtttcat gtgaaatgct ttgattttta tattttctga tacttcgtct ttttgatata
     1141 gaatcactta tcattttaag taacctccgt ttattttttg gaaaaatcta tttaattttt
     1201 attttctatt ttaattattt tagcattaat cagaaaaaaa gtcattgatt ttagtataca
     1261 attattaatg attatattaa gaattgtatc aaaaagtatg tgattatact aagggattat
     1321 attaaacatt atatttaaa
//
LOCUS       DAEOSP010000346         1337 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.351, whole genome shotgun sequence.
ACCESSION   DAEOSP010000346 DAEOSP010000000
VERSION     DAEOSP010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.351"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            240..1193
                     /gene="blaCDD"
                     /locus_tag="KRH54_003560"
     CDS             240..1193
                     /gene="blaCDD"
                     /locus_tag="KRH54_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG8581186.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYLGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEDISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLINKVDAKYVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT          570 a          108 c          225 g          434 t
ORIGIN      
        1 gtggaatcaa ttattatatc acaaattaaa aggaggacag gatgttatta atagttacct
       61 aattatcttc gcaataggtt taatcaacat aattgtacta ggtgaagaaa atataagaga
      121 tacagctatt catttagtct tgtttcttat aagtagaacc tagattggag tatattacaa
      181 ataatgtata ctattttaat agtaggttat aaatttaaat atgggagatg atataaatta
      241 tgaaaagaaa aaagaatttt atttggatag ctatcttatt ggttggtgta gttatggtaa
      301 tgtattattt agggaaaaaa cataacgata taaatcaaaa aactgataag aattatataa
      361 aatctgaatt aaataagtct gaattaaata aatccaaaat tgaaaacaat gataaaaaca
      421 aaaaagaaaa aattaatatg gttgactata gtgattgttt tgaagatatt agtggaggag
      481 ctatttttta taatactaaa aataaagaat ataatatata taacaaagaa ttaattgaaa
      541 caagaaggtc accatgttct acttttaaaa tagtttcaac tttaattggt ttagaaaaag
      601 gggtaataaa ttcaaaagaa tcagttatgg gatatgatgg tacagagtat ccaaataaaa
      661 attggaataa aaatcttagt ttagaagagg catttaagga gtcttgtgtt tggtactaca
      721 aaaaattgat aaataaagtt gatgctaaat atgtccaaaa cattttagat gatttaaagt
      781 atgggaattg cgatattagc gaatgggaag gagatttaaa aaatggaaag ggacatttaa
      841 atggattttg gttagaatct tcattacaaa tatctccaaa agaacaagtg caaacaatgg
      901 caaaaatatt tgagggagat acaaacttta aaaaagagca tattaatatt ttaagagata
      961 ttatgaaaat agatgtaaat gataaaaaca taaatgtata tggaaaaact ggaacaggat
     1021 ttgatgaaaa aaataaacgt gtggatgcat ggtttgttgg tatgctagag cgtgaaggtg
     1081 acacctatta ttttgctatt aaatcagatg attccaataa agaaattaca gggccaaagg
     1141 taaaggaaat tgcaattaat ataattaaga aatattatag tgtaagggaa taaacttaat
     1201 tttatttgta aatattattt acctatagtc tgtaatattt attaaataat tttactaaat
     1261 tgagtctata tagagcaaaa ctcctttagt acataaaata cttgctattg attattaaat
     1321 tgaataccta tagattt
//
LOCUS       DAEOSP010000347         1302 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.352, whole genome shotgun sequence.
ACCESSION   DAEOSP010000347 DAEOSP010000000
VERSION     DAEOSP010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.352"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(287..1168)
                     /locus_tag="KRH54_003561"
     CDS             complement(287..1168)
                     /locus_tag="KRH54_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBG8581187.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
BASE COUNT          439 a          242 c          138 g          483 t
ORIGIN      
        1 tttttaatca aatttaaaca gaataattat accaatatat aaaaaaattt ctaaaaaaca
       61 ataaaattaa gttataaaca ccaatatatt tctattattt caataagtat acaattagat
      121 tagaatctaa cgctagtaat aaaaaattac tttcccaaaa tagatataag cctcatttta
      181 tcgttcaaat aatgaaaata tatttgattt catgcaaaaa gagtgcctaa tgaactaaaa
      241 agttcatttt gagacactca tttttttata taaatttata tagtttttaa tcgttcgcat
      301 tcaaccactc agttaagact tttatatatt tctcattttc ttcaacgaat ggcatatgtc
      361 ttgaatactc aaataattcc cacttactat taggtattct atcatacata gtctttgcaa
      421 tatatggtga acataaatct attgctccac tagttactag acaaggctct tttatttcat
      481 gtaatctatc tgtaaattca tatccagaaa gagttcctgt aggagaaaat tcattttgtc
      541 cccatcctac aatatatgct tcagaacctg attttttagg tcttctcagg cactctggag
      601 aatcttctgt cacttcacct gcacaataca tttccataaa tctttctaat gcatcattat
      661 attctttgct agagtaatct ccagtattta ctgcatcaag taatgctttt tggtcaacct
      721 cactcatata tgatattctt cttttttgtt ctttctccca aagttttgca gaagaaagag
      781 tagatgaaag gatgtaagac ttaatacctt ttggtttata ttcaatagca taccaaatag
      841 cctgcatacc tccccaagat tgacctaaca gatgaatttc atcaagtcct agatgttttc
      901 ttagttcaat aagctcctct atccaagtat ctgcattaaa tagctcagga tgcccttcta
      961 caaatgaatt tccacaacca atttggtcat acattattac ttgtctacca ctttcagcta
     1021 tcttatccaa tacctcaaaa tagttgtgtg tagaacctgg acctccatga agtaatacta
     1081 atggcttctt cccttccgta ttttctccta ctatacggta atacgtttta aaacctttaa
     1141 aaggcatata tccttcagta attttcatgt ttatctcctc caatttgtaa ttataaacaa
     1201 taatcaagtt cattaacaat actatttaaa acctcttata catatttgat tatgtatttt
     1261 aatctttata cctactttta taaatagtat aacataaaag tt
//
LOCUS       DAEOSP010000348         1269 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.353, whole genome shotgun sequence.
ACCESSION   DAEOSP010000348 DAEOSP010000000
VERSION     DAEOSP010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.353"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..414
                     /locus_tag="KRH54_003562"
     CDS             <1..414
                     /locus_tag="KRH54_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBG8581188.1"
                     /translation="ADCYYKSAKAGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKN
                     YSSAFVSAPDWVSAKGMRILGNPLRGDEKVISGESGAVTTGALVSILESEDLKDLREA
                     LKLDENSKVLLISTEGDTDPDKYRDIVWNGECQSK"
     gene            679..>1269
                     /locus_tag="KRH54_003563"
     CDS             679..>1269
                     /locus_tag="KRH54_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG8581189.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT          550 a          134 c          255 g          330 t
ORIGIN      
        1 gctgattgtt actataaatc agcaaaagct ggagatggaa aaccaagatt tgttggagga
       61 gatatgccaa ctataatggc agggcttgct tgtggagaac caaacactat aggttttgaa
      121 gtgttaaaaa attattcatc agcattcgtt tcagcaccag actgggtatc tgctaaaggt
      181 atgagaatat taggaaaccc tctaagagga gatgagaaag taatctctgg agaatctgga
      241 gcagttacaa ctggagcatt agtttcaatc cttgaaagtg aagatttaaa agacttaaga
      301 gaagctttga aactagatga gaactctaaa gtattattaa taagtacaga aggagatact
      361 gaccctgata aatatagaga tatagtttgg aatggtgaat gccaaagtaa ataaaagact
      421 taaggaaaag aaaaacttaa taaataaatg aaaaagtaaa gcaaataaaa aagtaaagca
      481 aatgaaaaat tcaaaataaa aactaaataa aattaaataa aaccaaataa aattaaaata
      541 aagtctaaat aaaaatttaa taaataaaat ataaagaaat aaaaaatcaa tagattaagt
      601 gaaatgctaa taaattaaaa aagtgaaata tcaaaataaa acgtcaacaa attaaaaatt
      661 atatggggga atatcaaaat gttaaatgaa acaagaaaaa aacaattaac tgaagtatgc
      721 caagacttaa taagaaatgc tagttattca ggtcaagaag aaaacgtagt aaaagctata
      781 gaagaaaatt ttgctttact aggttttgat agttggtcaa gagatagata tggaaatata
      841 gtaggatgta taaaaggaaa taaaccaggt aaaaaaatat tatttgacgg acatatagat
      901 acagttccag ttccagatgc aagtaaatgg agtgttcctc cttttgaagc gagaatagta
      961 gatgggaaaa tatatggtag aggaacttct gatatgaagg ggcaagtaag tgctatgatg
     1021 gctgctgttt catactttgc agaagatact aacaaagact ttgaaggtga attgtatgta
     1081 gcaggtgttg ttcatgaaga aatatttgaa ggtgtttcag ctagagagat aagtaaggct
     1141 gttcaacctg attatgttgt aataggtgaa tcttcagaat taaacctaaa gataggtcaa
     1201 agaggtagag gagaaatagt agtagaaact tttggtaaac cagctcactc tgctaatcca
     1261 gaaaaaggt
//
LOCUS       DAEOSP010000349         1263 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.354, whole genome shotgun sequence.
ACCESSION   DAEOSP010000349 DAEOSP010000000
VERSION     DAEOSP010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.354"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            125..>1263
                     /locus_tag="KRH54_003564"
     CDS             125..>1263
                     /locus_tag="KRH54_003564"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBG8581190.1"
                     /translation="MFYIGIDVGKFNHCACVISSEGEVLIEPFFFTNNLDGFDSFIKA
                     VKKFKSPRHIVGLEATGHYGDNLINFLITHDYEVALINPLTTDAKRKSKIRKTKNDKK
                     DTFLICSVLFDRDYTRVTQRKLDMKQARELTRYHASIAEDINQKKNQLQKCIDIVIPE
                     FNTWFKTKYSKAYMAVLKEFGSSYNLASANLAHLKKVLKPKSSGSRVNIDASSLRSSA
                     KKSIGENNEAVTLEIKMLIENIELLASQLKTVDKKIEELAVKLDSPIFSIPGIGIYTG
                     MSILSEIGDIHNFSSAVKVMGFAGVDPGTYQSGEYSAPRTALSKRGSRYLRKSLYQCI
                     LTVCKYNLTFNKYYKLKRLQGKSHRCAQGHSVRKLIRVIYKLLSE"
BASE COUNT          412 a          224 c          218 g          409 t
ORIGIN      
        1 ttgtatctta attgtgatca gttagataag tcttcgaatt tgtattttgc accaggctct
       61 ttgatactct gtttgtggtt taatcacata tcactatctt atataaagaa aggatgtgct
      121 ttttatgttt tacatcggta tagacgttgg taaattcaat cactgtgcct gcgttatttc
      181 ttctgaaggt gaagtcctta ttgaaccttt tttcttcact aataatctag acggattcga
      241 ttcttttatc aaagctgtta aaaaatttaa atcacctaga catattgtcg gtttagaagc
      301 taccggtcat tacggtgata atcttattaa ttttttaatc actcatgatt atgaagttgc
      361 tttgataaat ccattaacaa ctgatgccaa gcgcaaaagc aaaataagaa agactaagaa
      421 tgataaaaaa gatacttttc ttatttgttc agtactcttt gatagagatt atactagagt
      481 cactcagcgt aagctcgata tgaaacaggc tcgtgaatta acccgttatc atgcttctat
      541 tgctgaagat atcaaccaga agaaaaacca acttcagaaa tgtattgata tcgttattcc
      601 tgagttcaat acttggttta aaactaaata ttccaaagct tatatggctg tcttaaaaga
      661 atttggaagt tcttataatt tagccagtgc caatcttgct catcttaaaa aagttctcaa
      721 gcctaaaagc agtggaagcc gtgtgaatat agatgcatca tctcttcgtt ccagtgcaaa
      781 gaaatctatc ggcgaaaaca acgaagctgt tacccttgaa atcaaaatgc ttattgaaaa
      841 tatcgaactg ttagccagtc agcttaaaac tgtagacaaa aaaatagaag aattagctgt
      901 caaactagat tctcctatct tctcgattcc cggtattggg atttatacgg gaatgtcgat
      961 tttaagcgag attggtgata ttcataactt cagctctgct gtcaaagtca tgggatttgc
     1021 aggtgtagac cccggtactt atcagtccgg tgaatacagt gcacctcgaa cagcattgtc
     1081 taaacgtggt tcgcgctacc taagaaaaag tctatatcaa tgcattctta cagtatgcaa
     1141 atacaatctc actttcaata agtattataa actaaaacgc ttacaaggta aatcccatag
     1201 atgtgctcag gggcattccg tacgaaaact cataagagta atctataaac tgttgtctga
     1261 aaa
//
LOCUS       DAEOSP010000350         1258 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.355, whole genome shotgun sequence.
ACCESSION   DAEOSP010000350 DAEOSP010000000
VERSION     DAEOSP010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.355"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..1184
                     /locus_tag="KRH54_003565"
     CDS             <1..1184
                     /locus_tag="KRH54_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBG8581191.1"
                     /translation="DKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEEL
                     TEGEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVE
                     EGEVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFE
                     VYYQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVC
                     NDLYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAI
                     NELNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDER
                     SRNIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDL
                     L"
BASE COUNT          477 a          109 c          234 g          438 t
ORIGIN      
        1 tagataaacc atctttaaat tatattatag tatattttga tattaataac tttaagatga
       61 ttaatgatac ttttggatat gaatttggtg acaatttatt aattacaata gcaaaagctt
      121 taaaagaaga attaacagaa ggtgaggtat atgcacgact ttcaagtgat tattttgcaa
      181 ttttctgtga ttataaaaat ggaagaaata aaattataag aaaacttgat aatatacgaa
      241 gtaatattga aagtaatttg agtatagtct ttgaaatttc tctgtgtgtg ggtatttatt
      301 ttgttgaaga aggtgaagta gatattcaaa aggctgtaaa taaagcaaat atggctaggt
      361 ctgttgctaa aggtaaaaat ataaattatg ctatttataa tgaagatgtt aggaataagc
      421 ttagtgagga atcaatgatt ttagatgata taaaaatagc attggtaaaa aatcaatttg
      481 aagtgtatta tcaacctaag ttttcacttg taactggaga aatgataggg agtgaagcac
      541 taataagatg gaatcatcca gaacatggtt ttataagtcc cgcagtattt attccaatag
      601 cagagaaaag taagttgatt ttaaaaattg ggagatttgt atttgaaagg gtttgcaatg
      661 atttatatga atggaaaaag caaggtaaaa agatagttcc agtttcagta aatttatcta
      721 gggtcgagtt gtatcaacca gatattgtaa aatttatcaa taaaacaata aaaatgtata
      781 atttaagttc agatttcatt gaaatagaga taacagaaac tgtggctatt aatgagttaa
      841 atattttaaa gaatgtttta aatgagttga ggacgtatgg attttctatt tctatggatg
      901 actttggtac aggatattct tctattagtt gtttaagaga tatgccaatt gatatattga
      961 agctagataa gtcttttctt ggtggcattg aacatgatga gagaagtcgc aatatagcaa
     1021 aatcaatagt gtctctagca aaatcactag atttagttgt aattatagaa ggtgttgaga
     1081 gtaaagaaca agctgagtta atgaaacaat ttggatgtga tttagttcaa ggattttatt
     1141 ttgcacgacc aatgccagct aagaattttt tagatttact ttaaaacaaa atttgatttt
     1201 tatataaagc actattatga atgcttgatt ttaataaatc attgctaaat aattagtg
//
LOCUS       DAEOSP010000351         1249 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.356, whole genome shotgun sequence.
ACCESSION   DAEOSP010000351 DAEOSP010000000
VERSION     DAEOSP010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.356"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(15..227)
                     /locus_tag="KRH54_003566"
     CDS             complement(15..227)
                     /locus_tag="KRH54_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8581192.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            complement(228..644)
                     /locus_tag="KRH54_003567"
     CDS             complement(228..644)
                     /locus_tag="KRH54_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581193.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            complement(712..1125)
                     /locus_tag="KRH54_003568"
     CDS             complement(712..1125)
                     /locus_tag="KRH54_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581194.1"
                     /translation="MSKSELGVKTNLVSELLMISYFFIDSLLKKFIFKEGNLCITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSILLIAVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIHFDRKGICN"
BASE COUNT          481 a          192 c          120 g          456 t
ORIGIN      
        1 ttataaactt tccactattc ttcttcaata aattcaaaca aattttctac tgtagtatcg
       61 aatactttag cgatatccat agctaacttc aaagaaggat tatatttccc attttctaag
      121 tgtacaatag tctcacgtct taccccaacc aatttagcaa gttcattttg cgtcataagg
      181 agtttttctc tgtattcttt aatttttgtt ttcatcaata ttgccactta gttacatatc
      241 ccccttttat caaagtatat atacaacact aatttagata aagactgtat aaataaaact
      301 gcaatgagga ttatacctgt atctaggttt gatatattta tattacttgt ataaggtgtt
      361 gcaaagaaaa tagatattat tactatagaa tatatcatta tttttattgc aatcttatta
      421 acctttgata gtataaattc agatgattca tcaactaaat cattatattt tttattataa
      481 attgaataac atccaaaaac tattaaccaa ctcaaccctg gaagagaagc agtgcttgga
      541 cctttaaaaa tatacttagt ggctaataca gctataaaaa aataagttat tacaagcaac
      601 tccaggataa gctttgatct tacaccagtt tctgatttag acatataaaa tcactcctta
      661 aaaattatat tctctatact ctttaatttt cgtttttatc aatattatca cttagttgca
      721 tattcccttt ctatcaaaat gtatatacaa aattaattta gataaagatt gtataaataa
      781 aatagctaag agaattacac ctatatccaa atttgatata tccaaacctc ttgtataatt
      841 tgctgaaaag aaaacagcta ttagcaaaat agaatataac attatttttg atgcaatatg
      901 atcaaccttt aataacataa gttttgacgc ctcgtcaact aaatcattat attttttatt
      961 atacatgaag tcacatgccc aaaaagcgaa taaaattata aaagtaatac aaagatttcc
     1021 ctctttaaaa ataaactttt ttaataatga gtctataaaa aaataggata tcataagtaa
     1081 ctctgaaaca agatttgttt taacaccaag ctctgattta gacatataaa atcacccctt
     1141 aaaagttata tatttataac accttatgtt ataaatatat aacttttcca aataaattgc
     1201 aatagaatat tgaaaatatt caataatatc ttcataaata tattttata
//
LOCUS       DAEOSP010000352         1208 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.357, whole genome shotgun sequence.
ACCESSION   DAEOSP010000352 DAEOSP010000000
VERSION     DAEOSP010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.357"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            74..805
                     /locus_tag="KRH54_003569"
     CDS             74..805
                     /locus_tag="KRH54_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581195.1"
                     /translation="MLSKKNVSLIILVIILSLLTAIFIFNNKNTNNKLKEVTKNNEEI
                     VPKNLEGLSIEQLIEIRDKAIDKNLEGLREFGEDSVKKRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQNYQVNEFLLKNGKDYKEWSY
                     EGLDFLIDHLEELCKFVDFDVKDVIDIIDPTIKTDLSEEQKKSLNDKLKKISSSDTLS
                     EEVKKEIKNWENNLNNLNMNKNNSN"
     gene            complement(962..1168)
                     /locus_tag="KRH54_003570"
     CDS             complement(962..1168)
                     /locus_tag="KRH54_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581196.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
BASE COUNT          503 a          129 c          149 g          427 t
ORIGIN      
        1 gtagtaaatg ttatcttcat aaacaaattt atttttcaca agtacaatac ttatttaaaa
       61 aaggaggttt aatatgttaa gtaaaaaaaa tgtaagttta attattttag taataatttt
      121 aagtttatta actgctatat ttattttcaa taataaaaat actaacaata aattgaaaga
      181 agttactaag aataatgaag aaatagtacc taaaaactta gaaggtttaa gtatagaaca
      241 attaatagag attagagata aagccattga taaaaattta gaaggtctta gggaatttgg
      301 agaagattca gtaaaaaaac gtaaagattc aattttaaac tttgaatttg gattagtagc
      361 ttttgttaac aagaattata gcaacttaac agaaaaagag aaattaataa gacaaattaa
      421 tattgttaac tatgaaaact ctataaatga tataatggat ccttcaggtt cattctttga
      481 attagcaaat cagaattacc aagtgaatga gtttctatta aaaaacggaa aagattataa
      541 agaatggtca tatgaaggtc tagattttct aattgatcat ctagaagaac tgtgcaaatt
      601 tgttgatttt gatgtaaaag atgtaataga tattattgat cctacaataa aaacagacct
      661 ttcagaggaa caaaagaaat ctttgaatga taaattaaag aaaatatctt caagtgatac
      721 tttaagtgaa gaagttaaaa aggagataaa aaactgggaa aacaatttaa ataatttaaa
      781 tatgaataaa aataattcta attaattttc tcataagaaa aatgcttata tcgtgataat
      841 gtgaaatgta cataattata aacaaaaaag gggctgtgac actaaaaaat agtgctacgc
      901 ccttttttat ttgtaataat catcaataca aattatttta ttatatgtaa ttatttatgt
      961 cttaaccaat tttatcgaaa ataacagtct tctcttttaa agagattttt cctacttgat
     1021 atccatattc atttaattcc tttttatatt tcaaaaacga atggtctatc tctatcccag
     1081 caatatcttt tatttcttca aaagttaatt taaacgattg actattgttt ttcttaacat
     1141 attcccataa agaactatat ttactcatct tatatcactc cttttaaaat catatcttat
     1201 ctattaat
//
LOCUS       DAEOSP010000353         1198 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.358, whole genome shotgun sequence.
ACCESSION   DAEOSP010000353 DAEOSP010000000
VERSION     DAEOSP010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.358"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(225..1094)
                     /locus_tag="KRH54_003571"
     CDS             complement(225..1094)
                     /locus_tag="KRH54_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBG8581197.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITPEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKALGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT          458 a          182 c          111 g          447 t
ORIGIN      
        1 tcgtaaataa taatctaaaa tacatcaaaa aataaactaa aaaatatcga aaaataattt
       61 aaaagacatc aaaactaaat cttctaatca gcaattaaaa gtctattttc ttagtttaaa
      121 aaataaagat agatttttta aaaacactgg ataaataggt aaaaaaataa acagttagta
      181 aaataaattt ttactaactg tttattttta ttgtttaata tgtcctataa atcctctaag
      241 taatactttc cttgcaaaaa ttctgattct aaacctagat ttttaaaata atcaaccatg
      301 cccatacacg caaatttttc tgtagaataa tgagtagcac ctataacatt aattaaatta
      361 tcttttgcta tttgatggaa ctctaatact ggttcaaaat gttttaaagg cttagtaaac
      421 ccagtaatat atagatttat accaagttca gcaacttctc ttgctacaaa tggataactt
      481 cctccccctg ctgcaatagc aactaaacca tcttttaata cagaatctcc ataatcatac
      541 aatttcacat catgaccaac agatttttta attaattcct taatctcttc tattgatttt
      601 agtgatgttt tacatataac tcctacctca attttatcgt catatttaca aaatggtttg
      661 actatttcta aacccagagc ttttgcaaaa ctaacagaag ttgaatagtc actatagttg
      721 tctagtggag aatgtaatac ataaaaagaa attctacgat ttttcatctc ttccatataa
      781 gatagtggta tgtcgtgaaa tggaaaacct tcatctgatg ctatgtatcc cattgcatga
      841 tgtgaaaaaa ttaaaatatc tttttcatct ttatctataa cttgcttaat tatttcttta
      901 tcaggaaaag tagttgtgta tactttatta atattttgag cattatctaa tactaatcct
      961 atgtattttt ttctaaattc tggagtaata aaatcatttt caaaattcat aaaactccaa
     1021 tcatcattaa tatttttaat attaaattct aaatctaatt ttttataaag ttcaattgcc
     1081 ttaataataa ccactccttt ataataatat tgaattacta catattaata tagaaatttc
     1141 cgataataaa ctttatacgt ttaagtattc ctacttcaaa ttaaaaatat tataaata
//
LOCUS       DAEOSP010000354         1187 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.359, whole genome shotgun sequence.
ACCESSION   DAEOSP010000354 DAEOSP010000000
VERSION     DAEOSP010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.359"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            14..1111
                     /gene="dinB"
                     /locus_tag="KRH54_003572"
     CDS             14..1111
                     /gene="dinB"
                     /locus_tag="KRH54_003572"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBG8581198.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
BASE COUNT          471 a          124 c          217 g          375 t
ORIGIN      
        1 aaaggtgata ataatgaaac attatagaaa aataattcat atagatatgg acgcttttta
       61 tgcatctata gaacaaagag ataataaaaa attaaaagga agacctgtta ttgtcggtgg
      121 aaatccacaa agccgaggtg tggttgcaac gtgttcttat gaagctagaa aatttggtat
      181 ccattcagct atgccatcag ctgtagctta caataggtgt ccatatgcag tttttgtaag
      241 acctagattt gatgtttata agagtgtgtc agagcaaata agagatatat tttatagata
      301 cacagattta gttgaaccat tatctttaga tgaagcttac ttagatgtaa caaaaaataa
      361 aaaaaatatt gattcatcaa ttgagatagc aaagcaaata aaaaaagata tatttagaga
      421 agttggtctt acttcttcag ctggtgtgtc atacaataag tttcttgcta agattgcatc
      481 agacttaaga aagccaaatg gattaactgt aataaccgaa gaaaatgccc aggacttttt
      541 agataaatta cctgtgaata aattttttgg tgttggaaaa gttacttcaa atactcttaa
      601 aaatttaggt ataaaaactg gatatgattt aaggtgttta aatttatttg aacttgaaaa
      661 tatattcaaa aaaagaggtt atgaattata taaatttgct agaggaatag acgatagacc
      721 agtggaacca aatagggtta gaaaatctgt tggggctgag actacattaa gtcataattt
      781 ggatattgat gaagaagaaa cacgcaacat tcttgatgaa ctttgtgaag aagtatgtca
      841 tagattaaaa aactctgaga agtttggaaa aacattaaca ttaaaaatta aatatgagga
      901 ctttacaaag ataactagaa gccttagttt agaacactat attgacgaat ataatgatat
      961 aagaagtggt gtagataatt tgttgagaaa tgttgaggtt aatggaaaac aaattaggct
     1021 attgggagtt acaatatcaa acctaagtga taaaaaagaa acttataaag atattacatt
     1081 atttgaatac atggatagta tccaaatgtg agttttgttt aaatgaaata acactagctt
     1141 taatactata taattgaggc tagtgttatt ttatgaatat ttttata
//
LOCUS       DAEOSP010000355         1183 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.360, whole genome shotgun sequence.
ACCESSION   DAEOSP010000355 DAEOSP010000000
VERSION     DAEOSP010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.360"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            450..602
                     /locus_tag="KRH54_003573"
     CDS             450..602
                     /locus_tag="KRH54_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581199.1"
                     /translation="MKNLNKNTILGEVIITIAACDDEKEIQIVIKNQVDNILKNKNKE
                     YRIKKI"
     gene            919..1164
                     /locus_tag="KRH54_003574"
     CDS             919..1164
                     /locus_tag="KRH54_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBG8581200.1"
                     /translation="MLIHTKNKTFDAICILEKIEKDLNIEELVRCHKSYIINLNYVEN
                     IKSNTAILDSQEEVPISRYRYKDIKERFLKFIGDRIC"
BASE COUNT          581 a          143 c          127 g          332 t
ORIGIN      
        1 taccatttta agataatata tttaaaaatt agagaataag aatgtacata cttgaatctc
       61 ataaatgtat ataaagatat gtatgaataa aaatagcagc tacttagaat agttacacta
      121 tttaagattt attatattat agattgacta aacaatagat atttattaat gactcaattt
      181 ataaacttta aaagttatac cccaataata ccccaaccac aaaaataaac aataaaaaat
      241 aaccaaaaaa atcagtctaa tatcataact aaatagctat atacctacat tcacaaaaac
      301 caacaaaaca atcaaataac cacttataga ctcccctcgt atccaccatt gaaatccacg
      361 aatacctata tccaattgga tataggtatt tttatacatt tttaatttaa atattattga
      421 taaatacatt taaaaagtgt taacctaaag tgaagaatct aaataaaaat acaatactag
      481 gagaggtcat cattacaata gcagcttgtg atgatgaaaa agaaatacaa atcgtaataa
      541 aaaatcaagt tgataatata ttaaaaaata aaaacaaaga atatcgaatt aaaaaaatat
      601 agctcaaaag aagaaccatt acaaagttaa ttgagaggat acactactct tagatataaa
      661 aatgccagaa ctaaatagta tagatatcgc aagaaacata agaaaagtag ataataatat
      721 ttataacatc tctaataaat tatgtacagg aaagatatga agtaagagct tattgatact
      781 tattaaagcc aataaatttt caagaactag aaaaacacat attaacatgt atcaaaaaaa
      841 ttgaaaaaaa taaaaataag tctaacacat acaaaatcta ctctagcaaa aaaatagata
      901 gaagttcaaa aaaagaacat gctaatacat acaaaaaata aaacttttga tgctatatgt
      961 atactagaaa aaatagaaaa agatttaaat atagaggaac ttgtaagatg tcataaaagt
     1021 tatataatta acttaaacta tgtagaaaat ataaagtcaa atactgcaat attagacagt
     1081 caagaagaag tacctattag cagatataga tataaagata taaaagagag gtttctaaaa
     1141 tttattggtg atagaatatg ctaaaccata taaataaaat att
//
LOCUS       DAEOSP010000356         1181 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.361, whole genome shotgun sequence.
ACCESSION   DAEOSP010000356 DAEOSP010000000
VERSION     DAEOSP010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.361"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            53..199
                     /locus_tag="KRH54_003575"
     CDS             53..199
                     /locus_tag="KRH54_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581201.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(304..828)
                     /locus_tag="KRH54_003576"
     CDS             complement(304..828)
                     /locus_tag="KRH54_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8581202.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(881..1105)
                     /locus_tag="KRH54_003577"
     CDS             complement(881..1105)
                     /locus_tag="KRH54_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581203.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
BASE COUNT          367 a          198 c          105 g          511 t
ORIGIN      
        1 ctagatattt gtcaataata tctatatgta ttatagaata ggaggcttaa atatggatat
       61 ggatttactc tttaaaatag tatttgctat agttgctata ggtgtttctg taaaaatcat
      121 taaaactgtt gctagtttag cttttaaaat agcgttgata ttgctaataa ttttatttgt
      181 atatagaatg tttttgtaaa catttaaaaa taagtttttt aacacaatag agaagtaaaa
      241 tagattatct ttaatgtaaa ttttttatta aagacactct attttacttc tctattagct
      301 tatttaaatt aaatttttat tatatcttcc cataatataa acatcttcaa attttccatc
      361 tctgattaca gcatattttt tagttccctc tatttcaaaa cctagctttt tatataaatt
      421 taatcccttt tcattatcca caattacttc taatcctact ctagttaaca taatccaatt
      481 atctgctata tctaaaagtt gttccataag cttcttacct actcctcttc cctgataatt
      541 ctctgcaaca cttattccta tttctgcgct atgtctcaat cgtggagaag aatttacatg
      601 taacccagca aaacctatta catctttttc tccattatta ttagtttcag ctacaaacat
      661 ataatcattt tctcccaaag aagctaacat agcttcagtt ttatcgactc tgtcactcat
      721 taatgccaaa gtatttctca taacactgta tgttcttcta atttcattaa aagcttgtgc
      781 atcttctctt cttataggtc ttattgttat atctataggc ttactcatat tggattcccc
      841 ctcaaacata atctttattt ttaattaaaa ttttacatct ttattttata ctgttcaaat
      901 ctatgtatgc ttctacctga ggtacatata tcatattatc ttttatatgt gtacttccaa
      961 attcttttaa ttctgtacct tctttatcct tagttatttt atatctaatg gattccatac
     1021 cttcaactac ttcagcatga aaaaacatac cttcatattg tttaaatcta aaattaaaat
     1081 tgtctttagt aagttttatt atcatatttt tatctactcc ttatataatt gatatttttc
     1141 ttattattag ttgtatatat actgcttatt catattaata t
//
LOCUS       DAEOSP010000357         1174 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.362, whole genome shotgun sequence.
ACCESSION   DAEOSP010000357 DAEOSP010000000
VERSION     DAEOSP010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.362"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(137..706)
                     /locus_tag="KRH54_003578"
     CDS             complement(137..706)
                     /locus_tag="KRH54_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBG8581204.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            909..1136
                     /locus_tag="KRH54_003579"
     CDS             909..1136
                     /locus_tag="KRH54_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBG8581205.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
BASE COUNT          395 a          167 c          156 g          456 t
ORIGIN      
        1 aataaaatta tataattaaa aatactctat aaaagctaaa aagtttttct attcgctaca
       61 tctaaaaata attagataga ggtataaatg tatttagttt atttatacct ctagtctttt
      121 ataaattata tctctactaa attgattttt tagcacgaat aagtgtgctc ttggagatac
      181 cagtcatctt cacaacctct ctatatggct tagaatttaa taaccccaat gcatattgta
      241 tctgttgatt gctgtatttc tttggcctac catctttaaa gtcctttttc tttttagcaa
      301 attcttttcc taattgagta cgttctatta tcatatttct ctcaagttct gcaactgcaa
      361 gtaatgtagt caaaaagaat ttacccattg aagtattttc taaaaggcct atatttaaaa
      421 tatgaacact tacttcttta gcaaataaac cttctattag ttgtattcct tctgatgtac
      481 ttctagctag tctatccagc ttagttgcaa caagtaagtc tccctttttt agagatgata
      541 taattttttc aaatttaggc ctgtttgtag tagtgcctgt ataagtttcc tttattattt
      601 tagcatcatt atatttattc aaaatttctt gctgttgctg atacaaactg ttgttgtctg
      661 tttgattttt tgtacttaca cggcaatatc catatataat aatcatcact tcctaacata
      721 aagattttgt acagaagtta cccagaagct tgtttttact tatctttatc cattttacta
      781 aaacgatttt tgggtgtcaa tactgttaac ttttgacacc ataataattc ctttataatt
      841 tgtttgacta tgtcaaaaat taccaatata atgaaattaa tgagtttata caaattggag
      901 gaaataatat gaatagtctg ttaaaagaag caatagatga attaaaaaac atccattgcg
      961 gggaagtttt tttattaaaa gaattgttca aaggctatga atggaacagg ttggagatga
     1021 atatcagatt gaaattagga aaactttttt tgtatgaagt ggtaactaat cccaatttaa
     1081 atgtagaggt catcaataaa aattctgctt atcagcaaat gtataaaaaa aaatagtgac
     1141 ttaaatgtat ataatagtat aatcttctta ttga
//
LOCUS       DAEOSP010000358         1135 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.363, whole genome shotgun sequence.
ACCESSION   DAEOSP010000358 DAEOSP010000000
VERSION     DAEOSP010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.363"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            4..213
                     /locus_tag="KRH54_003580"
     CDS             4..213
                     /locus_tag="KRH54_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBG8581206.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            213..479
                     /locus_tag="KRH54_003581"
     CDS             213..479
                     /locus_tag="KRH54_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBG8581207.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            508..1080
                     /locus_tag="KRH54_003582"
     CDS             508..1080
                     /locus_tag="KRH54_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBG8581208.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
BASE COUNT          409 a          173 c          198 g          355 t
ORIGIN      
        1 atcttggaaa ataagactag agatatcaaa aaaaattgtg gtccatcttt ggctagacca
       61 tacattaata atcaaatttt cacagaaatg tactgccttt ctgatgcatt aaaatttgga
      121 actatatttc cagaactaaa cttactagag tctgaaatgt acaacaaaga attatatgct
      181 aagccaaaaa aatatagagg aggtagaaga taatgaaaga ctcatctaga gctgaactta
      241 tgagaaaagt tcaggaaaca tcttttgcat gtgttgatat gaacttatat ttagacaatc
      301 accctgatga taaaaatgct atcaacactt ataattcatt atgtaatcaa tttgcgcaag
      361 ctagatatgc ttacgaaaat aaatacggtc ctttaactaa ctttgggtat gctccaagta
      421 gatgcccttg gcaatgggct gaccaacctt ggccttggga cagagaattt aattattaga
      481 catcaagtat tggaggattg aaaattcatg tggatatatc agaaaactat acaacaccca
      541 gttaatataa aaacttgtga ccctagaatg gctaaatttc ttataactca atttggtggg
      601 ccaaatggag aacttgctgc atctttaaga tatttaagcc aaagatatac aatgcctact
      661 ggtaatatgc gtgcactttt aacagatatt ggtacagaag aactagctca cgttgagctt
      721 atatgtacta tggtttatca gttaacttct gatgcaagcc cagaagagtt aaaagctgca
      781 ggtcttggtt caaactatgc tcaaaatgga tatggaattt atccaacaga ttcaaatggt
      841 gttccatttg atgtaagacc tatagcagtt atgtcaaatc ccgtaaccga tttacatgag
      901 gatatggcag ctgaacaaaa agcacttgca acttattatc aacttataaa cctaacagat
      961 gacgttgatg ttatagatgt attaaaattc ttgggtcaaa gagaaataat tcattatcaa
     1021 agatttggtg aagccttaat ggatgcttac gagttagaag aatctcaaaa aatgttctaa
     1081 aatttttatt tgtataacta ttattatttg ataatttata atttactgtt gtgtt
//
LOCUS       DAEOSP010000359         1131 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.364, whole genome shotgun sequence.
ACCESSION   DAEOSP010000359 DAEOSP010000000
VERSION     DAEOSP010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.364"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..414
                     /locus_tag="KRH54_003583"
     CDS             <1..414
                     /locus_tag="KRH54_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HBG8581209.1"
                     /translation="ADCYYKSAEAGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKN
                     HAAAFVSAPDWVSAKGMRTLGNPLNGDEKVISGESGAVTTGLLVAAMEREDLADLRKD
                     LKLDENSRILLISTEGDTDPDKYRSIVWDGEYPSI"
     gene            541..>1131
                     /locus_tag="KRH54_003584"
     CDS             541..>1131
                     /locus_tag="KRH54_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBG8581210.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT          435 a          122 c          251 g          323 t
ORIGIN      
        1 gctgattgtt actataaatc agcagaagct ggagatggaa aaccaagatt tgttggagga
       61 gatatgccaa ctataatggc agggcttgct tgtggagaac caaatactat aggatttgaa
      121 gtattaaaaa atcatgcagc agcatttgtt tcagcaccag actgggtttc tgctaaaggt
      181 atgagaacat taggtaatcc tttaaatggt gatgaaaagg taatttctgg agaatctgga
      241 gcagttacaa ctggtctttt agtagcagct atggaaagag aagatttagc agatttaaga
      301 aaagatttaa aattagatga aaattcaaga atacttttaa taagcacaga aggagatact
      361 gaccctgaca aatatagaag tatagtttgg gatggagaat atccaagtat ataaggaatt
      421 actaaaatgc aattattaga tattggttta catatttaga agatatataa gttctaaaat
      481 ttagaaaata tataagtata taaaatacat acattataaa aaatctaggg ggataatgaa
      541 atgttaaacg aacaaagaaa acaagaagta actgaaatat gccaaaaatt aattcaaaat
      601 cctagttctt ctggaaatga agaaggtgtt gtaaaagcaa tagaagagtc ttttgaaaaa
      661 ttaggttttg atagttggtc aagagataga tatggaaata tagtaggatg tataaaaggt
      721 aacaagccag gtaagaaaat attatttgat ggacatatag atacagttcc agttccagat
      781 gcaagtaaat ggagtgttcc tccttttgaa ggaaaaatag ttgatggaaa gatatatggt
      841 agaggaactt ctgacatgaa aggtcaatat actgcaatga tgtctgctgt agcgtatttt
      901 gctaaagaca ctaatagaga ttttgctgga gaactatatg tagcaggtgt tgttcatgaa
      961 gaaatatttg aaggtgtttc ggctagagag ataagtaagg ctgttcaacc tgactatgtt
     1021 gtaataggtg agtcttcaga gttaaactta aagattggtc agagaggtag aggagaaata
     1081 gtagtagaaa cttttggtaa accagctcac tctgctaatc cagaaaaagg t
//
LOCUS       DAEOSP010000360         1121 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.365, whole genome shotgun sequence.
ACCESSION   DAEOSP010000360 DAEOSP010000000
VERSION     DAEOSP010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.365"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(269..985)
                     /gene="dapD"
                     /locus_tag="KRH54_003585"
     CDS             complement(269..985)
                     /gene="dapD"
                     /locus_tag="KRH54_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBG8581211.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
BASE COUNT          394 a          190 c          124 g          413 t
ORIGIN      
        1 cactttatta aattataaaa agtttaatag tttattacat tgatatattg aaaaattgaa
       61 ggaaataaaa atacttgtca gcaagaaata aaaatgatat aagaatgaat aagagaattg
      121 taaaaaaata aaatgattat aaataaaaga taaaaatatt gtaaaaaaga gggtgcctct
      181 ggtgaattaa attcacacag agacaccttt ttaattaatt ttttaaatta gtatattatt
      241 gtttaattaa tagcatagta ctatttaatt agtctaaatt tctaaggtct tccataagct
      301 tagttttgtc agccgttttt tcatctttca tttttataac tttagcagga gaaccagcaa
      361 caacggcacc agcttctaca tcagtagtta caacagcacc agctgcaaca acagcacctt
      421 taccgattct aacaccttca agaataactg cattagctcc tattaaaaca tcatcttcaa
      481 ctataacagg agtagcagaa ggtggctcta aaacaccagc aacaacagca ccagcaccta
      541 agtgaacatt tttaccaaga gttcctctag ctccaataac agcattcata tctaccatag
      601 aaccttcacc aataacagct cctatattta taacagctcc catcattaca acagcatttt
      661 tttctatagt aaccatgtct cttattattg cacctggctc aattcttgca tgttcactta
      721 aagtattttt tagaggaata gcagaatttc ttctatcata ttctacatga gtatctgtta
      781 tatcatttcc atgtttatct aatacttcca ttatatcctc atagtcacct acaaatatat
      841 aagaaccatt acttccaaat accttaaatt tatcagttga ttggaagtgt aaagagttag
      901 tatttacata taccttaaca ggggttttct ttttaacttc ttttatatac cttgctattt
      961 cataagcatc gtttaaattt accatttgtt aattctcctt gtatttcatt atatttcttt
     1021 tattatatac ttatttgtca attttagtat atacttactt cttattattt atattttatt
     1081 tagtttttat tgtatagaca tagtattaaa taaatttatt a
//
LOCUS       DAEOSP010000361         1121 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.366, whole genome shotgun sequence.
ACCESSION   DAEOSP010000361 DAEOSP010000000
VERSION     DAEOSP010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.366"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(261..1007)
                     /gene="cdtR"
                     /locus_tag="KRH54_003586"
     CDS             complement(261..1007)
                     /gene="cdtR"
                     /locus_tag="KRH54_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HBG8581212.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT          417 a          138 c          117 g          449 t
ORIGIN      
        1 ttatatgata tgtcagtaca aaataacaac tgaatatttt aaagagacta tttcgtactt
       61 ttatgattta atgagctatc ttatcgaatc ataatctagg aacaattgtt ataatatcta
      121 atagtagtta tatatgatat agtcagtatg tgttttttca caatatgatt attatataga
      181 ggattcttag ggattactct ttttgtataa aaaagagatg gttcaaattt ttatcttgag
      241 acatctcctt tttctattta ttatgtttta ataatattct ttaaaatatt tctatatgta
      301 ttagatatac gacatatatg gccattactc attttaatat tatttttctt taggtcgaga
      361 gaaacaatat gatatttatt aactacaaaa gatttatgta cacggataaa gcatgcatct
      421 aaatctggta aagactttaa acttttatag aattcaaata ttctagaagt agtcactaat
      481 tttattttat gtggaatagt gcttgtttca aaatatataa cttcatcaaa tgaaattttc
      541 catagtgaga aatctgaata gatagtaaaa aaattagagc gataattttt ttctttaatg
      601 tatcttttgt gagcaatttt aatacaagct tttattttac tttcagcaat ttccatatca
      661 tgttttagta taaagtccat aacttctaac ctataatcga agatccctaa aatcatttct
      721 atatgatttg taagaaatat gattggacta atataatctg attctctaat tttttgtgct
      781 atccataatc catcacataa ttcattactt atcttatatt ttgcgtctac aactataaaa
      841 tatattctta ctttattttt ttcagtaagc tcttttaata acaaatttgc attatgatat
      901 aatcttacaa catcattatc aaaaccttct tttattaaaa tattatttat tttttctttt
      961 agtttcattc caaaacaaac atcattatca aagattaata tatccactaa ataccctcct
     1021 ataaaaaatt caaaagtaaa aaaacaaaag tattttatag aaaatacata aaataacact
     1081 aggagataaa taagattaag gatagttaga attaaggtat a
//
LOCUS       DAEOSP010000362         1114 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.367, whole genome shotgun sequence.
ACCESSION   DAEOSP010000362 DAEOSP010000000
VERSION     DAEOSP010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.367"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            292..990
                     /gene="tcdC"
                     /locus_tag="KRH54_003587"
     CDS             292..990
                     /gene="tcdC"
                     /locus_tag="KRH54_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HBG8581213.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
BASE COUNT          442 a          119 c          182 g          371 t
ORIGIN      
        1 cacctctaaa tcactgagtc acttaattac atcataatat agttttatac aaataaaata
       61 ctttatgttt aatttaatat ataaaattca ccttcaagaa aattatatta taatctgaca
      121 tttttatctt attttttcaa aatatattga atcttcttac tttattttgt aaaattatgc
      181 ttaggggaaa tatattttag gaaaatatga atatataatt tttagtcaac tagttatttt
      241 aagtttttaa cttttaaaat aaaatatatc taataaaagg gagattgtat tatgttttct
      301 aaaaaaaatg agggtaacga atttagtaat gaaggaaaag gaagctctaa gaaaataatt
      361 aaattcttta agagcacaaa gggtattgct ctactggcat ttattttagg cgtgtttttt
      421 ggcaatatat cctcaccagc ttgttctgaa gaccatgagg aggtcatttc taaccaaaca
      481 tcagttatag attctcaaaa aacagaaata gaaactttaa atagcaaatt gtctgatgct
      541 gaaccatggt tcaaaatgaa agacgacgaa aagaaagcta ttgaagctga aaatcaacgt
      601 aaagctgaag aagctaaaaa agctgaagaa gctaaaaagg ctgaagaaca acgcaaaaaa
      661 gaagaagagg agaagaaagg atatgatact ggtattactt atgaccaatt agctagaaca
      721 cctgatgatt ataagtacaa aaaggtaaaa tttgaaggta aggttattca agttattgaa
      781 gatggtgatg aggtgcaaat aagattagct gtgtctggaa attatgataa ggttgtacta
      841 tgtagttata aaaaatcaat aactccttca agagtattag aggatgatta cataactata
      901 agaggtataa gtgctggaac tataacttat gaatcaacta tgggtggaaa tataactata
      961 ccagggatag ctgtagagaa aattaattaa tattgttttg tattatagtt aatattttat
     1021 attatagtca atatgtttaa agatgttttt ataattgcaa ataaacagtt acaaggctct
     1081 aaattagttt ttgcttttag catattatct attt
//
LOCUS       DAEOSP010000363         1062 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.368, whole genome shotgun sequence.
ACCESSION   DAEOSP010000363 DAEOSP010000000
VERSION     DAEOSP010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.368"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(337..>1062)
                     /locus_tag="KRH54_003588"
     CDS             complement(337..>1062)
                     /locus_tag="KRH54_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBG8581214.1"
                     /translation="YVSAYHKENLEAKSANKVYKIGGGLTSKVMSSIASSLSKHNTTP
                     TEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIREKDYTLNTSLATTEYLRSKGFNVIM
                     TRDTDKTLSLGNRTALSNSLRPDLFTSIHYNASDTTGNGVEVFYKLKDKDGGTTKTVA
                     TNILNRILEKFNLKNRGAKTRTLSTDPTKDYLYVLRNNDMPAVLVECAFLDNEKDMSL
                     LNTSNKVKEMGTQIGKGIEDSLK"
BASE COUNT          326 a          186 c          123 g          427 t
ORIGIN      
        1 attatctata ctatatcatt tattgacata aaaatccaca aaaaacacac aaacacacaa
       61 taaaatatag taattaagct tttattgtgt attacaaatg aatactccaa ctcttgacaa
      121 ttaactgtag ccttaagata tgtaaattct tcatataata taaatccttt tacaattaat
      181 cataaaaaga ctgtatatac ctcatttcgc acttatatgc acaatgtata tacagtcttt
      241 attactatta taattacatt cttatcttat agctttagtt taaatttaaa attgcagatt
      301 aatttatttt aaatttttct atgctctcat tgtagactat tttaatgaat cttctatgcc
      361 tttacctatt tgtgtaccca tttcttttac tttatttgat gtattaagta aactcatatc
      421 tttctcatta tccaaaaatg cacattctac aagtacagct ggcatatcat tatttcttaa
      481 aacatataaa taatcttttg tagggtcagt tgatagtgtt cttgtttttg cacctctatt
      541 ttttagatta aatttctcta agattctatt taatatattt gtagctacag ttttagtagt
      601 ccctccatct ttatctttaa gcttataaaa tacttctaca ccattcccag ttgtatcaga
      661 tgcattatag tgtatactcg taaataaatc tggtctcaaa gagttagata aagcagttct
      721 atttccaaga gataaagtct tatctgtgtc tcttgtcatt attacattga atccctttga
      781 acgtaaatat tcagttgtcg caagtgaagt atttaatgta tagtcttttt ctctaatacc
      841 ccctaatggt ttacctgttg ttccagtgtc cgaaccacca tgtcctgggt caatcataac
      901 tgtcttgccc ccactatttc ctggttctgt tggagttgta ttgtgtttag ataatgatga
      961 tgctatagag ctcattacct tagaagtcaa tcctccacct attttatata ccttatttgc
     1021 cgatttagct tctaagtttt ctttatggta tgcagataca ta
//
LOCUS       DAEOSP010000364         1054 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.369, whole genome shotgun sequence.
ACCESSION   DAEOSP010000364 DAEOSP010000000
VERSION     DAEOSP010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.369"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(101..811)
                     /locus_tag="KRH54_003589"
     CDS             complement(101..811)
                     /locus_tag="KRH54_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBG8581215.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT          366 a          144 c          112 g          432 t
ORIGIN      
        1 taatcttcaa aaaattaaat ttgaaaaaac aaaactctaa aaactaagct ttaaaaaatt
       61 aagtttcaaa aataaaattc tagagattga acttcaaaaa ttaagattct aacatatacc
      121 ttgtaaattt tcctacaacc tcttgatatt ttttacggct tataggaatt ttttcattct
      181 gaactttggc ataagtttga gttatactat ctacatggtt tagattgaca atataccttt
      241 gatgcgtacg tataaaatta gagggaagaa cagtttccat atcatttaat tttccgtagt
      301 attctaatgt tttatcaaag gtaactacag ttaattttct tagctcactt ttaaagtaga
      361 ggatatcttt tgtattaatt ttacaaagat tagttccaat ctgaactgta ataaagtttt
      421 ctttccattg ttctaatttt tttagtgctt cttttaatac tttacatatt ttttcagttt
      481 tataaggttt taagatgtag ttcaatgctc ctacatcata tccatgaaat acatagtcag
      541 aaaaacctgt gacaaagatg attatagtat cctttgaatg ttttcgtata gtttttgcta
      601 tgtcaacgcc attacttttt ccaagttcaa tatctaaaaa aataatatct atgtaataat
      661 tttcgtgttc tagattgtat actagacttt ctccatttga atattctttt atttggtagg
      721 ggtagcccca caaatccatt tctcttgtaa tgattttatt cagcatgttt ctttgttgtt
      781 tttcatcatc acaaatacaa atcgaagtca attttttatc tcctttcaaa ttttatattt
      841 atatagttaa gtatataata tattttgcta cctagatata taaaatgaaa taacaattat
      901 atccagatag caaaataaaa ttttttatat tagtatactt acacttaaaa ctattctatt
      961 aaacttattc tgaagtgcta atgattttct tattaaaaaa tctaacaatc tttcttatta
     1021 taaaatacaa atgcttttat tattataaaa agta
//
LOCUS       DAEOSP010000365         1040 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.370, whole genome shotgun sequence.
ACCESSION   DAEOSP010000365 DAEOSP010000000
VERSION     DAEOSP010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.370"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            49..1005
                     /locus_tag="KRH54_003590"
     CDS             49..1005
                     /locus_tag="KRH54_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBG8581216.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
BASE COUNT          426 a           85 c          157 g          372 t
ORIGIN      
        1 gattataaaa aatttttaga atgtaaattg aaatttagga gtagatgtat gtatatactg
       61 aatttaaata caagagagac tattgaagat tttagagata aattttatgt agcagaaaat
      121 tcttatctta tattaagtgc tccaaaaaat ttaaaattgc ttaaggaaac tttggatata
      181 gatgaaataa cttttaatga ttgtcttaaa tttgatgaaa taaccaaact tgatttattt
      241 gataattacg attttttaag cctaaataca tttgaactaa gagatggaga agctgttata
      301 gaagaggtaa atatgtattt atcagataat tttatattgg ttgtagtgaa tgaagaacac
      361 tttttatttg aatttgtaaa aaatattatt ttgaagaact ctcaacttga aaaaaatcca
      421 gttattaatc tatttaagat aaactacttg attcttagag aagttataaa aaatggtttt
      481 gagtctttgg aaaaagttga agagttaatt cttcaaatag aagatgaaat gatggacaac
      541 attaataaga atcatgtatc tagaataagt gatgtaagag gattaacaag aattatagtt
      601 aagaatacta gacctctttt gtatattggg gatagaatag ttaaggaaaa tataagatat
      661 ttaaaatatt caaatgtaaa aaaatataat ttagaaaatt ttcaaggtat agattttgga
      721 attgacaaat tatacaattt cgcattgtca acacgagagt tagcagataa attattagat
      781 atttattctt ccagagtagg tgagaaaaca aataacttaa ttacaaaatt aaccttgtta
      841 acagccatat cagcaccact tacaattata actggtatat atggaatgaa ctttagatat
      901 atgccagaat taaactggat ttatggatat ccagcaactt tattttttat gctatgtata
      961 atttttgttg gaattataat atttaaaata aaaaaattat tgtaaattac agatgatgtt
     1021 taattgattt tataaaaata
//
LOCUS       DAEOSP010000366         1032 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.371, whole genome shotgun sequence.
ACCESSION   DAEOSP010000366 DAEOSP010000000
VERSION     DAEOSP010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.371"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(143..946)
                     /gene="proC"
                     /locus_tag="KRH54_003591"
     CDS             complement(143..946)
                     /gene="proC"
                     /locus_tag="KRH54_003591"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBG8581217.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSELVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT          323 a          205 c          109 g          395 t
ORIGIN      
        1 aataaattct cactcaatca ttaaattcct ttttatagta aattactctt attttactta
       61 taccaaatac atttatgtat agatataaat aagcactaaa aaaacaattc aagaaaccat
      121 atagtaaact taattacatc tgttatttgc tcatatcttt agacttatct atacaagcaa
      181 caatagattt tataactgct gctctaaagc cttcttcttc taatacctta acagcttcta
      241 tagtagttcc tgctggagaa caaaccatat cttttaattc acctggatgt tttccagttt
      301 caagaaccat cttagctgac cccattactg cttgagatgc aaatttataa gcttgttgtc
      361 ttggcattcc agctaatact gctgcatctg ccatagcttc tataaacata aatacatatg
      421 caggagaaga accactaata cctgtaactg tatccattaa atattctgat actatttctg
      481 cctcaccaaa agattcaaac agagccttaa tcatatttaa ctcttcatct gttacattac
      541 tatttttaca aagagctgac attccttcgc caacaagggc tggagtatta ggcattactc
      601 ttactatttt tttatctgaa cctataaaac tttccagact ttctatagtt ttacctgctg
      661 ctatagatac tattatttta tttgcatcta ttaattcttt aagtggagtt aaagcctttt
      721 cgcatatatt aggtttaagt gctactatta ccacatcaga atttgtaaca gctacctttg
      781 aatcagttgt agtatttact ccaaattctt cttttacttt atctaaggca acttggctta
      841 aatctgatac agttatatct tctgccttta ctaattctga ctgtactatg ccacctatca
      901 ttgcacttcc catattccct gaaccaataa atcctaaagt tttcatatca aatctccttt
      961 tttctattag ttttatttta aagtagctaa attgacttta ttttaactgc ttaaaattat
     1021 cttattttta ta
//
LOCUS       DAEOSP010000367         1007 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.372, whole genome shotgun sequence.
ACCESSION   DAEOSP010000367 DAEOSP010000000
VERSION     DAEOSP010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.372"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(155..646)
                     /locus_tag="KRH54_003592"
     CDS             complement(155..646)
                     /locus_tag="KRH54_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBG8581218.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASNDGLAVIF
                     FIM"
BASE COUNT          377 a          153 c           89 g          388 t
ORIGIN      
        1 tatttataaa gacataagac aaatttacct acagatttaa atttaactta tatattttaa
       61 aaggctaata taatatgtat tttcttctat acaatcaaaa ggcttcatat ctaagatatg
      121 tgatgacact aatattcaca atttttagta attatcacat tataaaaaat attacagcta
      181 gtccatcatt agatgcttct tgtataaact ttttaagctc aattagattt gcccatacat
      241 attcaaaaaa atcatcttca ctctcatcat tcataacagg ataaatatta ttttctaaca
      301 tctcattaat attaaatctt tttctaaatg cttgttcttc caaactgttc attgcttttg
      361 atagtttttt tacatcattt gctgtaatca acatgacagg aaattctcca tcaatttcca
      421 taagtatgtt tccacttagt actaatttgc taaatatatt atcatcatct ccaccaaaag
      481 gacaacctgt caaagtaaag tgaatggcat gccatgcttt atcaatattg atagtactat
      541 tttcgttggc atcttcaaaa acaaaatctt gtaaattttc ttctgcttgc tgtaatttaa
      601 aaacattctc ttcacttatt ttagtataac atcctatcat ccccataatt acattcctcc
      661 taagtagcgt ttttttaatt gtaacttata attaaactta gttacaataa aatatattga
      721 gaaaagtatt atttcatctt aaatttaaat acctatatta tcaactaaaa ttctgtgttt
      781 atagcgtaag tattattatt ttttcctaaa aatcaaatga atcagaagtt ctataggcaa
      841 tattgactat aaataagaac tattactttt aaaaaaaatt taagaaatta tactgtagaa
      901 tcatgctcta cttattagct tcaaataaaa aataaatcat gttctcaaat taaataataa
      961 ataacaataa atttaaattt taaactataa gagtttctga agtgtta
//
LOCUS       DAEOSP010000368          997 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.373, whole genome shotgun sequence.
ACCESSION   DAEOSP010000368 DAEOSP010000000
VERSION     DAEOSP010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.373"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            286..936
                     /locus_tag="KRH54_003593"
     CDS             286..936
                     /locus_tag="KRH54_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBG8581219.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
BASE COUNT          426 a           97 c          170 g          304 t
ORIGIN      
        1 cgatttaatc tatagtataa tagtttaatt tgaagtacaa tggtttaatc tatagtataa
       61 tagtttaatt tgaagtacaa cgatttaatc tatagtacaa taacttgatt tgcaatacaa
      121 tgaattaatc tatagtataa gagcatgatt tacagtacaa taatttaaat tgtagtacaa
      181 aagtactatc aaataagaat ttctacttta aaaatattac taaaagtaat taaagtccag
      241 taattcaata taatatggca aaagaataga aataaggtga gagtagtgaa agtaaagtca
      301 aatttagatg aaattttata tcataagatt attgaaagtc ttattcgtgg tgaatactct
      361 gtaggtcaaa aaatattact aaatgacctt tgtgaaaaat ttgaagtaag cagaacacca
      421 gttgtacaag cagtaaaaat gctaaataaa gatggagtgc ttactattat gacaaatggt
      481 aaagtgtacg taccagagta tgaatatgat atggtaaaac aagtttgtga aacgagaaca
      541 ctgattgaaa catatgcttt agaaaaaatg atgcaagaag aggaagaggt ttttcaacaa
      601 aagctagatg taataaaaag atactctgat aaatgcgagg agtactatcg tcaagaaaaa
      661 tctgtagaac ttgcactagc agatttggaa ttacataaag ccattgtaga aggggcaaat
      721 aatgagattt taaatgatgt atatgttggt atacaaggaa gatttatagt agtgaactat
      781 ttaattcgac cattaaaaaa tcgtaattat gaaggaacag tacaagacca ttttgaaata
      841 ttaaaattca ttgaagacag agatacaaaa aaagcagtag ataaactaag aaatcatatt
      901 caaggaacta ttaaaagatt ctgtgaagat gaataataag ttttgaagtt actatatata
      961 cttatctatt aagttgctaa atgtagtcta agtataa
//
LOCUS       DAEOSP010000369          988 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.374, whole genome shotgun sequence.
ACCESSION   DAEOSP010000369 DAEOSP010000000
VERSION     DAEOSP010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.374"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..>988)
                     /locus_tag="KRH54_003594"
     rRNA            complement(<1..>988)
                     /locus_tag="KRH54_003594"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          220 a          291 c          223 g          254 t
ORIGIN      
        1 ctattagtat cgataagcta aatacattgc tgcacttaca ccttcgacct atcaaccaga
       61 tagtctttct ggggtcttac ccttgcggtg ggaaatctta tcttgaagtt ggcttcgcgc
      121 ttagatgctt tcagcgctta tccattccgt acatagctac ccagccatgc ccttggcagg
      181 acaactggta caccagaggt acgtccatcc cggtcctctc gtactaagga caggtcttct
      241 caaatttcct acgcctgcga cggataggga ccgaactgtc tcacgacgtt ctgaacccag
      301 ctcgcgtacc actttaatgg gcgaacagcc caacccttgg gacctactac agccccagga
      361 tgtgatgagc cgacatcgag gtgccaaacc tccccgtcga tgtggactct tgggggagat
      421 aagcctgtta tccccagggt agcttttatc cgttgagcga tggcccttcc acgcagaacc
      481 accggatcac taagtccgat tttcatcctt gctcgacctg tatgtcttgc aatcaagctc
      541 tcttttgcct ttacactcta cgtacgattt ccgaccgtac tgagagaacc tttgagcgcc
      601 tccgttacct tttgggaggc gaccgcccca gtcaaactgc ccacctgaca gtgtcccaag
      661 accagattca tggcctatgg ttagagtccc agtactacaa gggtggtatc ccaaggatgg
      721 ctccgccaaa actggcgtcc tggtttcaaa gcctcccacc tatcctgtac atgtagtacc
      781 aagacccaat gtcaagctac agtaaagctc catggggtct ttccgtcctg tcgcaggtat
      841 ccggcatctt caccggaatt acaatttcac cgagtctgtt gttgagacag tgcccaaatc
      901 gttacgcctt tcgtgcgggt cggaacttac ccgacaagga atttcgctac cttaggaccg
      961 ttatagttac ggccgccgtt tactgggg
//
LOCUS       DAEOSP010000370          960 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.375, whole genome shotgun sequence.
ACCESSION   DAEOSP010000370 DAEOSP010000000
VERSION     DAEOSP010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.375"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(180..863)
                     /locus_tag="KRH54_003595"
     CDS             complement(180..863)
                     /locus_tag="KRH54_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBG8581220.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
BASE COUNT          377 a          155 c           77 g          351 t
ORIGIN      
        1 ttgcctacta tacttaagac attttaatta atttatacac tgcttactat acttaaaata
       61 cttctattaa cttatacatt gcttactata ttttaaattc tttaattaat ctatacaata
      121 tttgctatat ataaagtata tcaaataaca gcttcatttt tttgtaaatt tacagaaagt
      181 taattttcat taaatgaatc atctttattg ataaacataa ttccaaagac cactgccaaa
      241 ataaaaaaac atattagtat tgatttttcc acgaaaattt ttgtaaaaaa agttcctaaa
      301 atagctccag atataaagaa tataataata gcataatact gtaaactctt ttttatcata
      361 aaaatattct tagtatgtat ataatgaaat aaagtttctg tagcacttct taaatttcca
      421 gtacacattg tcgttgcaaa actattccca tttacttttc taaaactctc tacttgcatt
      481 gaacaaataa aagaaatagc aacatttaca aacatattaa atcttcctct aggaataaat
      541 gaaacaataa gtattgtaat catttcaatt ataatgataa cctgacgcca atgaatcttt
      601 gtcttattat ttgtaaaata ctttcttatc ttctcggcta taaatacacc taacataaat
      661 gccaaaatag gaatcaaata ataaaatgat tcctttacat taccttctgc tatattaagc
      721 ccaaacaaaa ctatatttcc agtttgtgca ttagcaaaga ctttacctct taccacataa
      781 gtataaatat ccaaaaatcc tcctgttatt gccaacaaag aacataaaaa gaatgattca
      841 gacatctgac tcttgcttgt catatataaa cctctcgctt tctaatgata gatacaaaat
      901 caaaatcatt ttagattcta tttttcattg atataaaaaa attatttata tttaaatatt
//
LOCUS       DAEOSP010000371          957 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.376, whole genome shotgun sequence.
ACCESSION   DAEOSP010000371 DAEOSP010000000
VERSION     DAEOSP010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.376"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            297..764
                     /locus_tag="KRH54_003596"
     CDS             297..764
                     /locus_tag="KRH54_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBG8581221.1"
                     /translation="MKFLNVLVVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          369 a           64 c          161 g          363 t
ORIGIN      
        1 tagtgtatat taatattccc ttgaaattaa aaaaacatag atatttcagt aatctaaaat
       61 agtctacaaa aataacagtt ttagcaaaat taataagaat acatatagat ttaaacctaa
      121 aagtatgaac atgtctgttt tttacaagat taaaatttat attatatgat aaaattaact
      181 taactatttc aataagactt ataatttttt tctttatact agaccttatt gaaaccttta
      241 tagtttaatt ctaattatat attataaata aagtatgtgt atattaagga ggtaatatga
      301 agtttttaaa tgttttagta gtagttgaag atattgagaa atcaaaaaag ttttattatg
      361 atgttttggg gttaaaggta atctgtgatt ttggagaaaa tgttgtatta gaaggtaata
      421 tatcattaca agaaaaaaag ctttggttag aatttattaa taagagtgac agtgaagtta
      481 agttcaatgg aaatgatgct gagttatatt ttgaggaaga taattttgat acttttgttg
      541 aaagattgag tacaatgaaa gatatagatt atgttcatct agctatagag cataggtggg
      601 gtcaaagagc aattagattt tatgatttag atgggcatat tattgaggtt ggagaaacta
      661 tgtcatctgt gtgtagacgt tttttagata gtggtctttc aatagatgag gtagctaaaa
      721 gaatggatgt aactgtcgaa tatattgaat cagttttaga atagatggat tatattaaag
      781 taaaattgag gtgaaggctg taagtaaact tgtgtgaaaa caaatttgct tacagttttt
      841 atttttctaa aataaagaat ataatacttg tttagcttta tttgagtttt taaaaatatt
      901 atgatatttc ttatatattt atactaaagt ttaaatttag aaaaaatata taaaaga
//
LOCUS       DAEOSP010000372          953 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.377, whole genome shotgun sequence.
ACCESSION   DAEOSP010000372 DAEOSP010000000
VERSION     DAEOSP010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.377"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            19..771
                     /gene="cwlD"
                     /locus_tag="KRH54_003597"
     CDS             19..771
                     /gene="cwlD"
                     /locus_tag="KRH54_003597"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBG8581222.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT          415 a          103 c          152 g          283 t
ORIGIN      
        1 aaatgtttgt actaatatat ggaatattat agtgttttta taagtataca tatacagggt
       61 gatgttgtga gaaagtacat aaaacatata atttttagct ttgcaatgat atgtctagta
      121 gtagtatcca tatttgaaat taaaaatatt tctgaagatg ttatcaagta tatgccagta
      181 acaaacaaaa ctataatttt agatgctggt catggaggca ttgatccagg tgcattaaat
      241 aaggataaga gtacatctga aaaggatatt aacctagcaa taacacttaa gcttagagag
      301 cttatagaat caagtggtgg ccttgtaata ttaactcgag aagatgacag tagcctttat
      361 aaagaagaaa ataataaaac tacaagacaa aaatataatg agaatttgaa aaatagaaaa
      421 gaaataatat ctaattctaa tgccaatatg tttgtctcta tacatcttaa tgcatttgaa
      481 caatcaaaat actatggagc tcaaactttt tatcctaaag acaaacaaga cagtaaagaa
      541 ttatcgaagt gtattcaaga agaacttaaa agagtagtag ataagacaaa caacagagaa
      601 gtcaaaccaa gggatgatat atatcttttg aaagaaaata atattccatc agtactgata
      661 gaatgtggtt ttttatcaaa tgaaaaagag tgtaaactct taactgatga aacatatcaa
      721 gaaaaaatag catgggcaat ctacatagga atacaaaaat atttaagttg aaataactat
      781 aaaaataggt ctaaatatgt aaatagcttg tattaaagca gttaaaatgc attaatatag
      841 gctattttta ttttgacaaa aaaatattta aaataaaagt taaaaagttg ttgacttaga
      901 ataatataga tgatattata tatgagtgcc caaaaggagc accaaaataa gac
//
LOCUS       DAEOSP010000373          934 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.378, whole genome shotgun sequence.
ACCESSION   DAEOSP010000373 DAEOSP010000000
VERSION     DAEOSP010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.378"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            143..802
                     /gene="cprR"
                     /locus_tag="KRH54_003598"
     CDS             143..802
                     /gene="cprR"
                     /locus_tag="KRH54_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBG8581223.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
BASE COUNT          377 a           56 c          176 g          325 t
ORIGIN      
        1 gtatctaaaa tattttattt gtaaaatagt aattaattat tgtaagctat aatatgagag
       61 tattatggtt aatgtgaatt ttaaagatat tattaaatat ataaattaga atgctattag
      121 atggataaat ggaggagtta ttatgtctaa gatattagta gtagatgatg agttagatat
      181 gttaaagttg attgaaaatg tgttgaaaag agatggacat gaagtaaagg taattagtga
      241 tgtagaaaag gctatgaaca tggattttaa ttattttaat ctaataatat tagatgttat
      301 gatgcctaag atggatggtt ttgaagtatg taaaactata agaaataagg ttgattgtcc
      361 aatactgttt ttgacagcaa aaaatatgga aagtgatatt atgtacggtc ttggtatagg
      421 tgcagatgac tatataacca aaccttttgg tgtaggagaa ttaagggcta ggataactgc
      481 tcatttaagg cgagaaagta gagaaaaaaa gaatttatta actatctcaa atgttcaatt
      541 taacttttta ggcaaagaag tttcaattga tggtaaaaaa attaatttta ctaaaagtga
      601 atatctaata tgtgaatttc tagcaaaaaa taagggacaa gtattttcaa aagaaagaat
      661 atatgagtcg gtatatggtt ttgatggaga aagtgatata agtactatca cagagcatat
      721 aaaaaatata cgaagcaaat taaaacaata tgatatttat acaatagaaa ctgtatgggg
      781 gatagggtat aaatgggtgt aataagtata agtgttgttt agtgaaaata ttatatatat
      841 ctacaaaatg tttgtaaaat attaatctat atattaaaat ttaatttata gattaatatt
      901 ttttgtaaat gacaaaattg taaggtattt tgtt
//
LOCUS       DAEOSP010000374          933 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.379, whole genome shotgun sequence.
ACCESSION   DAEOSP010000374 DAEOSP010000000
VERSION     DAEOSP010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.379"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            415..885
                     /locus_tag="KRH54_003599"
     CDS             415..885
                     /locus_tag="KRH54_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBG8581224.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
BASE COUNT          422 a           63 c          166 g          282 t
ORIGIN      
        1 ttacttataa gtataattaa ggtagctttt taaatagagg tagtgtaatt aaagagaatt
       61 tttaaatata aattgtgaag ctttaatata gatgataata tatattttgc aaatatgaca
      121 cagtgttttc gtatttaaga tgataaaata aataggaatt aatgatgata ttgaaaaaat
      181 aaaaataaag gattaatata agatattaaa gagatagaga taaaagtgtt aacagaagat
      241 attgaaaaaa taaaagattg atataagata ctgaagaggt agaaacaaaa gtgttaatag
      301 aagatactaa aaaagtaaaa ataaaggatt aataaaaggt actgaagaga tagagaaaaa
      361 aatgttaata gaagatactg aaaaaataga aataaaaaat taggaggaaa atttatgaaa
      421 tatcaggttt cattattagc agtgaaggat attgaagtat caaaaaaatt ttacaaagaa
      481 ttatttgagc aagaaattga attggactta ggtaaaaatg taacatttaa aggaggattt
      541 gctatacaag aagacttcgc atggcttaca gggattaatc cagatacaat tatacaaaaa
      601 tcaaataata tggaacttta ttttgaggtt gatgattttg acgaatttat tgaaaaactc
      661 aattattata aagatgtaga gtttgtccat aagccactaa aacatgaatg gcaacaaaga
      721 gtagtgcgta tttatgaccc ggataagcat attattgaga taggagagtc tatggaagtt
      781 atagctagaa gatatttatc tgagggatac tctgtagaag aaacttctaa aataattcaa
      841 catccaatta aatttgtaaa gcagtgtgaa aatagtaagg aatgattgtt taaattataa
      901 ttatttagta gaaattaatt gtaatagaac taa
//
LOCUS       DAEOSP010000375          918 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.380, whole genome shotgun sequence.
ACCESSION   DAEOSP010000375 DAEOSP010000000
VERSION     DAEOSP010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.380"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            91..654
                     /locus_tag="KRH54_003600"
     CDS             91..654
                     /locus_tag="KRH54_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581225.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
BASE COUNT          366 a           89 c          137 g          326 t
ORIGIN      
        1 ttgtaaaacc acatttatta tgttagaata tcttatgata ttaaattgta agtgtataat
       61 attatttagt tactagattg gggggaaatg atgaataaag tctatattgt aacaacatat
      121 acaggaacta tattatctta cttaataagg aatataagta aaaagctgta tacacatgtg
      181 tcaatatcat taaatgaaaa tctaaaacca atgtattctt ttggaaggct aaatccaagg
      241 aatccattta ttggaggatt tgttgaagaa aatataaatc aaggattata tgctataaga
      301 aaaaacactg tgtgtagggt atattcgtta gaagtagata atttacaata tgaaaatcta
      361 tataaaaata taaaattaat tagtgattat agagaagatt actattacga tacaatggca
      421 ttaatatatt tactaatgaa tattcagaga caatctgaat ataaatatgt atgttcaaat
      481 tttgttgctg acatgttaga aaaaagtgag atagacattt taaataaaca agcatttgaa
      541 gtaacgccaa atgattttta taatttatct ggcttaacct tagagtatga aggtttatta
      601 tcagaatata attctagaca acattcttat gtatcaaaaa tcgcaaacat ttaaaaactt
      661 gatttagagt ttttcatata aaaaaatatt gctagataat taggctgaag atagtagttt
      721 aacttcgata ggattaagaa tttgtaaatc ttagttagaa tactttcttc agtttttaaa
      781 tttctgctaa tatttgtatt tttataatat ttttggatat atataataca gaatagtgca
      841 aaaatcccac tcctttaggt gcgggatgga tagtacaaac agtgtataaa aagtatttat
      901 acatacacgt ataatgtt
//
LOCUS       DAEOSP010000376          907 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.381, whole genome shotgun sequence.
ACCESSION   DAEOSP010000376 DAEOSP010000000
VERSION     DAEOSP010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.381"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(126..515)
                     /locus_tag="KRH54_003601"
     CDS             complement(126..515)
                     /locus_tag="KRH54_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8581226.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
BASE COUNT          295 a          148 c           76 g          388 t
ORIGIN      
        1 tttagattca cattatagta tcataatcca cttataatca tttgtcatat tacttaagta
       61 gactaatata ttacataaat acacttacat acactttata ttgatgctat aagaaatacg
      121 tttgcttata cgtctttctc ctcttctttt agatttttta tccactcttc aataagtaaa
      181 atatcatctt tgctcaaatt aatattttca tgtaatgaca acaataaact ttttatagaa
      241 gaatcatgaa tatttctaaa aaaatctctt gtctcaactc ctatgtattc cttttcttta
      301 attaaaactt cataatgcgt atatttatct atcttttgag aatttagaaa tctttttact
      361 actaatctag ataaaagagt caaagttgtc gtttgtttcc attgatattt ttgctccatt
      421 cctaaaacaa tatctctaga tgttactttg gaatctgatt tccatataaa tttcattatc
      481 tttaattctg attgtggcaa cttttttata gtcatgataa aaccttgcta ctcagaatat
      541 agacaagcaa tatccataat aaaatttttc gattaaatac atcttatacg gatttatata
      601 agctcagact tataagatat ttttcaatct taatcctaaa tttagctatt tgtacacggt
      661 aaatatatat aaataatgtc aaaattctct tgtattatat tgcttaattt tatgaaatat
      721 tttgtactca gctatctatt ttggatactt gtccagtagc tctcctttct tttaaactct
      781 ttgattttaa atatttgtat ttcagtaaat cctacttcct aaatttttta tttactacaa
      841 cttattactg aacttcctac ttcctaaact ttttatttac tacaacttat tactgaactt
      901 ctcactt
//
LOCUS       DAEOSP010000377          895 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.382, whole genome shotgun sequence.
ACCESSION   DAEOSP010000377 DAEOSP010000000
VERSION     DAEOSP010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.382"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(335..856)
                     /locus_tag="KRH54_003602"
     CDS             complement(335..856)
                     /locus_tag="KRH54_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBG8581227.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
BASE COUNT          298 a          170 c           69 g          358 t
ORIGIN      
        1 aaataaataa aactaaaaaa gatgttgtct caaaatatag ataaatttat ttttcattgc
       61 taaaatgatt aaaatatatt tgatttaata tacttgattt aatataaaaa aagagtgtct
      121 cataaactaa aaagttcact tttagacact cctttatata tattatctac ttcttaattc
      181 atttattacc tttctacatt ttctttcagc tagtggatac atctttataa gtactaatta
      241 ctccacttaa aaatggttcc tgtactgtcc atcagtcata tttttctgcc aaatataatg
      301 gtgtttcata atgagacaat aaatatttat atctctactt atcttctcta agctcgtcaa
      361 atccaactat aggtatctct aaattatatt ctttaagtag cttttctagc tcttctataa
      421 aaataccttt cccatccacc atttgtatat catttatttt atccatcaat tcattgcaac
      481 cacttaactc accaaaccaa cgtttagaac tacatgtctt attataacca caacttggac
      541 tgccatctat tgcaatgata ctttttatat cataatcatt ttttaaatat tcatcaaact
      601 gccttacatg aggtattaac atttgcctac attgttctct aaaaaacaaa ttgtcaaact
      661 gttcttttac atgtccccac ctatttattc catacataat catttctgga catggtagtt
      721 gaattattcc atacccttta tttattaaat ctattgctat attttgtgct cccttaaatt
      781 ctgacaatcc ctctacttta gaattacaat ttaatataca atgacatact actacaatct
      841 ctctatttct cttcataata atctccttag ttattcttaa ttttaatgat actac
//
LOCUS       DAEOSP010000378          872 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.383, whole genome shotgun sequence.
ACCESSION   DAEOSP010000378 DAEOSP010000000
VERSION     DAEOSP010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.383"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     repeat_region   269..829
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=336..364
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
BASE COUNT          369 a           97 c           97 g          309 t
ORIGIN      
        1 tgagtttatt aataagaaga ttagatactg caataacatc attagcatta gtatctatta
       61 taacaatagc atcatattat ttaatcagaa aaaatgtgta attatgtaag ctatttataa
      121 taaaacactg ataaaattaa taataaaaag gtaataggat ttctatacta ccttttggtt
      181 tttataatat agtgtaattt tattatattt ttgtatgcta atataactag ataatcataa
      241 gataacacat taaaagaaaa ttataaaaac ttacattctg tatagttaat ataaaacata
      301 agtaatataa atttatgtaa agcaacaaaa gatggattta cattccatat agttaatata
      361 aaacgatttc aaatgattag aataaaaata tcagaattgc tatttatatt ccatatagtt
      421 aatataaaac taagaatgga aaaggaaata ggagttcata ttccaacatt tacattccat
      481 atagttaata taaaactatg tatcccccat taaaaaggct tagattaatt tctatttaca
      541 ttccatatag ttaatataaa actcaaacat atctaagttg tatttctcac cttgtaggtc
      601 atttacattc catatagtta atataaaaca aggattttga atgtaattta tcataagggt
      661 ggcagattta cattccatat agttaatata aaacatgaaa gataattgga aaactaaagt
      721 gcaatctaaa aaatttacat tccatatagt taatataaaa cggagaatta ttaaaaggag
      781 ttcccgaagc agatagaata atttacattc catatagtta atataaaacc attgttatta
      841 ttgtggttct cccttagctc ataatttatt ta
//
LOCUS       DAEOSP010000379          864 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.384, whole genome shotgun sequence.
ACCESSION   DAEOSP010000379 DAEOSP010000000
VERSION     DAEOSP010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.384"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..801
                     /locus_tag="KRH54_003603"
     CDS             <1..801
                     /locus_tag="KRH54_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBG8581228.1"
                     /translation="KLKNLINDLSYDVDDREIVNLYKNILEEFNITKDIKLKYSYEVE
                     TPAFFNSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYI
                     MDKHIDLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIK
                     KRVMNMFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGFEDLRTVSSIRF
                     SVIGFTYADAPKNMREEYKINCESLGLIPKDSDKIEVGIEYYDSLLND"
BASE COUNT          345 a           77 c          131 g          311 t
ORIGIN      
        1 aaacttaaaa atttaataaa tgacttatct tatgatgttg atgatagaga gattgtaaat
       61 ctttataaaa atatattaga agaatttaat ataacaaaag atataaaact caaatactct
      121 tatgaagttg aaacaccagc attttttaat tcatgtgtac ttttgccacc acatgagtat
      181 aaattgaaag agttagactg gatttttaga catgagctta tgcattttaa gagtagggac
      241 ctttatttaa aatatgtaat cctgttttta aaaacagtat attggtttaa tccatttata
      301 tatataatgg ataaacatat agatttagat tgtgaacttt attgtgatga aagagtttta
      361 aaaaatcgaa attcagagga aaaaaaggat tatgctttaa caattctaaa tgcaatgaga
      421 aaaggttcaa atacttcaaa taaatttata gctggactcc ataagcaatc tgatattaaa
      481 aagagagtta tgaatatgtt taatgaaaag tataagaatg gtatattaat ggcattaact
      541 ttatgtttat tatcttctat tacttttttg aaggttaatt caactagtat tgttaatctt
      601 aatgtattac ctaaaggatt tgaagactta cgaactgtta gcagtatacg tttttcagtt
      661 ataggattta catatgcaga tgctcctaaa aatatgaggg aagaatataa aataaattgt
      721 gaatcattag gtttgatacc taaagactct gataaaatag aagttggtat agaatattat
      781 gacagtctat tgaatgatta aataaatatt attttaacag atattaatta taaagttttg
      841 aatagtaatt tgttgagaaa taaa
//
LOCUS       DAEOSP010000380          854 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.385, whole genome shotgun sequence.
ACCESSION   DAEOSP010000380 DAEOSP010000000
VERSION     DAEOSP010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.385"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            6..839
                     /locus_tag="KRH54_003604"
     CDS             6..839
                     /locus_tag="KRH54_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBG8581229.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIKMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDNRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
BASE COUNT          307 a          133 c          135 g          279 t
ORIGIN      
        1 aaaaaatgaa tgtagaaaat caatctttat cagatattga aataatatta tcacacaggt
       61 atgataatgg tgcagactat tggactacac ctgataagcg tttaataaaa ggttctcctt
      121 tctctactct tgatagtgta ctttatctat tagaattagg tgtgaaccct gatgatactt
      181 tacttaaaga atgtgctgac ctgatattta gcacatggcg agaagatgga cgtttcaaac
      241 tatatccaaa tggaggtatt tacccttgtc atactgcaaa tgctgctaac gtactctgtc
      301 atatgggata tgcatctgac attaaaatgc aaaaaacgtt tcaacatttt ttagatacac
      361 agtatactga tggtgggtgg cgttgcaata aatttagttt tggtcgaggt ccagaaactg
      421 aatactcaaa tccactacca actcttaata tattaaatgc attccgtttt agtaattatc
      481 taaataaaga atcaaaactt gacaaagctg tagattttct acttgaccat tggactatta
      541 aaaaaccaat cggcccttgt cactatggaa ttggcacatt atttatgcaa gttgaatatc
      601 ctttccgtaa ttataaccta ttcctttatg tctacgtttt atcattttat aatcgtgcaa
      661 aaaaggataa tagattttta gaagcattga agatacttga atctaaaatg ataggtaata
      721 aaattgtcgt tgaacgtgta gttccaaaac tatctaaatt atctttttgt aaaaaaggcg
      781 agtcaagtga aatagccaca acacactatt acgaaatatt aaaaaaccta gaaaaataat
      841 agttatatag ataa
//
LOCUS       DAEOSP010000381          833 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.386, whole genome shotgun sequence.
ACCESSION   DAEOSP010000381 DAEOSP010000000
VERSION     DAEOSP010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.386"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            210..716
                     /locus_tag="KRH54_003605"
     CDS             210..716
                     /locus_tag="KRH54_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBG8581230.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTIAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          338 a           82 c          132 g          281 t
ORIGIN      
        1 tatattatat actttataat caatatattt tatctatatt ttacacttat aaaatatgaa
       61 aactacttat tgcgaaagta attgatttgt tgtattataa tttacatgaa ttatgtagga
      121 caatcaaaag tcagtattat aagagtacat atattcaaat ttttaacaat taatttatat
      181 aattaaaaaa tgaagggtag gatgaagtca tgggttttag agaagtaaaa attgaggaat
      241 tacagtttaa tccatttact aagattggca aagaatggtt acttataaca gctggtgatt
      301 cagaaaagtt taacacaatg actgctagct ggggtggtgt tggagtctat tggggtaaaa
      361 atgttgttac aacttacata agaccacaga gatatacaaa agaatttgta gatagtaatg
      421 atacatttac aattgccttc tttgatgaaa cgtatagaga agctttaaat atttgtggca
      481 ctatttctgg tagagatata aataaaatag aaaaagcagg acttacacca tattttgtgg
      541 atgatacggt agcttttgaa gaagctaata tgattatagt atgtaaaaag ctatatcatg
      601 gtaatatgcc accagaaaac tttgatgcta aagaaaatga taaaaaatgg tatccagaga
      661 aagactatca tactatgtac atttcagaaa ttataaaagt tcttgtaaaa gaataattat
      721 aataaaaaat atatgaaaag aacttattat aagtaaatga ctgtgctttc ttatctgata
      781 tctaagtaga tgctactaac taaataaata ttaattttaa ataaatacta tta
//
LOCUS       DAEOSP010000382          828 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.387, whole genome shotgun sequence.
ACCESSION   DAEOSP010000382 DAEOSP010000000
VERSION     DAEOSP010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.387"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(146..535)
                     /locus_tag="KRH54_003606"
     CDS             complement(146..535)
                     /locus_tag="KRH54_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBG8581231.1"
                     /translation="MKINKLPHAELKLMKYIWGVDDVLASRDIIEDMKLKYDWKKSTT
                     LTFLKNLVDKGFLTTDKVDRCTHYTIAIKEKDYLKVETKSFFSFMHNNSFKSFISALH
                     DDEVLDSKSLDKLEEYFKNLKEEDIDD"
BASE COUNT          286 a          129 c           76 g          337 t
ORIGIN      
        1 ggtattattc tctatatttt ttactatcaa acactaagga aataatcact tttctttata
       61 taatgttcta ccatcacata aaactaacat taaatccaaa cgctactctc aattgtctca
      121 aactaaaaag atactgatag ctttattagt catcaatatc ttcttctttc aaatttttaa
      181 agtattcttc taatttgtcc aagctcttac tatctaaaac ttcatcatca tgtagtgctg
      241 aaataaaact cttaaatgaa ttgttatgca taaaactaaa aaaactcttt gtctcaactt
      301 ttaagtaatc tttctctttt atagctatag tataatgcgt acatctatct accttatcag
      361 tagttaaaaa gcccttatct actaaatttt ttaaaaatgt caatgttgtg ctctttttcc
      421 aatcgtattt caacttcatg tcttctatga tatctcttga ggctaataca tcatctactc
      481 cccaaatata cttcattagt tttagttctg catgtggtaa tttattaatc ttcattagtt
      541 tacacctctt accaatttat gtataactaa actttataac aataagtgta tgtaatcaat
      601 tagattggaa taaatataaa ttccaattta ttctatataa aaataattaa taaaaaagaa
      661 gtactctact ttagagagta cttttggatt atatattcaa aaatttatct aacatgatta
      721 gagtatgttt tatgctttag tgtgataatt ttagttcgtt ttattattgc aacatgtatt
      781 atatttcatt ataatctata agcttaaata caggatattt atttcata
//
LOCUS       DAEOSP010000383          789 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.388, whole genome shotgun sequence.
ACCESSION   DAEOSP010000383 DAEOSP010000000
VERSION     DAEOSP010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.388"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            74..772
                     /locus_tag="KRH54_003607"
     CDS             74..772
                     /locus_tag="KRH54_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBG8581232.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIIENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          297 a          107 c          127 g          258 t
ORIGIN      
        1 ctatattaca acattatttt atagtttgat taatttaaca atcactaata tatcaaactg
       61 aaaggagatt tttatggaaa aaaaatattt cgaaattcat ggagttcctt caatcctttg
      121 gggaaatcct tcaagtaaat tatatattta catacatgga caatgtggca ataaagagga
      181 ggctgaagct tttgctaata ttgcagttcc acatgagtgt caagttttaa gtgttgattt
      241 gcctgaacat ggagaacgta aaagagaaaa aaattcattt aacccatggc atgttgttcc
      301 agaattagta tctattatgg aatatgcaaa gtgccactgg aatcaaattt cgttatatgc
      361 aaatagcatt ggagcatggt ttagtatgtt aagctttgaa aaagagaact taagagagtg
      421 tatttttgta tctccaattc tagatatgca acagctcatt tcaaatatga tgctttgggc
      481 aaatgtatca gaagaacagc ttaaacatga actaattatc ccaacttcat ttgggcatac
      541 tctttcatgg gaatatctac aatatgtaaa acaacatcct atcactcaat ggaatgtacc
      601 aacaaaaata ttatatggta aacatgatga acttactgaa atcggtgttg tagagaaatt
      661 tgtagaaaag tttaattgca accttactat tatagaaaat ggcaaacatt ggtttaacac
      721 accacaagaa ttaaattcat tatatgaatg gttaaatact ttagttaaat aaaaaataat
      781 tatattatt
//
LOCUS       DAEOSP010000384          753 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.389, whole genome shotgun sequence.
ACCESSION   DAEOSP010000384 DAEOSP010000000
VERSION     DAEOSP010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.389"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..>753
                     /locus_tag="KRH54_003608"
     rRNA            <1..>753
                     /locus_tag="KRH54_003608"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          207 a          156 c          227 g          163 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gatattcctg tac
//
LOCUS       DAEOSP010000385          716 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.390, whole genome shotgun sequence.
ACCESSION   DAEOSP010000385 DAEOSP010000000
VERSION     DAEOSP010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.390"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..634)
                     /locus_tag="KRH54_003609"
     rRNA            complement(<1..634)
                     /locus_tag="KRH54_003609"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          173 a          182 c          127 g          234 t
ORIGIN      
        1 ctacttaacg taactcgttg gcccgttcta caaaaagtac gcggtcacac aagaaatgtg
       61 cttccacagc ttgtaagtgc agggtttcag gttctatttc actcccctcc cggggttctt
      121 ttcacctttc cctcacggta ctatacgcta tcggtcacta ggtagtattt agccttggag
      181 ggtggtccct cctgcttccc acagggtttc acgtgtcccg tggtactctg gatcatatct
      241 aaagtcttct cgtttaacat acaggactat taccttctgt ggtgggtctt tccaaacctc
      301 ttcaattacg atacctcttc gttatgatat gtccgcaacc ccaataaaga aaactttatt
      361 ggtttgggct aatccgcgtt cgctcgccgc tacttacgga atcgaatttc tttctcttcc
      421 ttcaggtact tagatgtttc agttccctga gttcccctca ttaagctatg tattcactta
      481 atgatactta gacattactc taagtgagtt tcctcattcg gaaatcttcg gatcaaagtt
      541 tacgtgcaac tccccgaagc ttatcgcagc ttatcacgtc cttcatcggc tcctagtgcc
      601 aaggcatccg ccctgcaccc ttaataactt gaccagttaa aaaggtttga tagattagaa
      661 gctatcaact tcgataattt taaaaagtta tcagctttat atattacaaa ttagat
//
LOCUS       DAEOSP010000386          713 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.391, whole genome shotgun sequence.
ACCESSION   DAEOSP010000386 DAEOSP010000000
VERSION     DAEOSP010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.391"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            98..>713
                     /locus_tag="KRH54_003610"
     CDS             98..>713
                     /locus_tag="KRH54_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBG8581233.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNK"
BASE COUNT          283 a           74 c          100 g          256 t
ORIGIN      
        1 aatttattat tgtaatattt tgaatatata ataaaattaa taataagaaa actttttata
       61 aatatacttt taattgttta aattggagga aaaaattatg gatgaaaaga taacaaaaaa
      121 taattataat gttccatttg actatgttcg aaaaaaactc aaagaattag accctcatac
      181 aattagtaat ttaagtaata tacctttcaa taatgattta aatagattca cactcaattt
      241 ttttgataga caatatatta tagactatcc aagtggagaa gtttggaata gtaatggtag
      301 cctttgcaat aactttgagc ttaaaatctt gatagttaga tatttaatca atgctaaagg
      361 tatccctaga actaataaat atgtaacttt taaagaaata ccaggaggca atgtttatta
      421 tccaaatttt ttaaacagga ctctatatag gctttcagaa gtttttacat ctcaaataga
      481 caaatataag ttagttatgg agaaaattgg agctgaaaaa gtaaacatgg gagatttagc
      541 ttataggttt acatatatgg gtaatacatc tatggtattt atactttggt ttggagatga
      601 tgaatttcct ccaaattcaa atatattatt tgattctaat atagtttatt acttcaatgc
      661 agaagattta gcagttgttc ctgatacagc tataaatgca attttaaata aaa
//
LOCUS       DAEOSP010000387          702 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.392, whole genome shotgun sequence.
ACCESSION   DAEOSP010000387 DAEOSP010000000
VERSION     DAEOSP010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.392"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(11..679)
                     /locus_tag="KRH54_003611"
     CDS             complement(11..679)
                     /locus_tag="KRH54_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581234.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSGKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
BASE COUNT          223 a          120 c           38 g          321 t
ORIGIN      
        1 attagaataa ttaaactttt aatttattgt tatttaatct attttgataa acattattta
       61 ttttattatt atttaaatct ttaaaatcta aacttttaca ttgttcatca tcttcccata
      121 ttcctgcttg tttatttcca tcatttttat ataatacacc cttaccattt ttcttattat
      181 ctaaaaaata tcccttataa atgtcattat tgggatatgt taattttccc aaaccatttt
      241 ttaatccgta tttaaattca cctgtatata ttgaaccatc tctataagta tattttccta
      301 ttccatgcat cttgtcatct ttccataatc cttcatactt ttccttacct gaaaaaatat
      361 atattccaaa tccatttttc tttccatcaa caaactcacc tttatataaa tcaccattat
      421 cgtatttaaa tactttttct tcctttaatt tcttattgtt actcttattt ttattattat
      481 tgttatcctt tatatgtatt tcttctcttc tggcttcaat aaaatctcta tcatacctaa
      541 atccgtttct tgatttaatt ttttgattaa tcttcttctt attaataaat actctagcta
      601 gttgagtaaa tataaataca gatgataagc atataaatac acatagtatt gttgtaaaat
      661 aactagttat attttccata aaaaatcacc ctttaaaaaa tt
//
LOCUS       DAEOSP010000388          688 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.393, whole genome shotgun sequence.
ACCESSION   DAEOSP010000388 DAEOSP010000000
VERSION     DAEOSP010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.393"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            91..>688
                     /gene="dptF"
                     /locus_tag="KRH54_003612"
     CDS             91..>688
                     /gene="dptF"
                     /locus_tag="KRH54_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-dependent restriction
                     protein DptF"
                     /protein_id="HBG8581235.1"
                     /translation="MSNGCLIDELKKLKESSKEAVENISDFSSFKKYMHVKRQVENEL
                     LDIIKYTADSNKAELILVCGGVGDGKSHLISYLNNKYPEIMSKFIIHNDATESFEPNK
                     TSIDTLNDVLDDFSDKKMDNSDKKLVLAINLGALSNFLESEYVNKFTKLREFVENKGI
                     LDISISDDDDKNSNFHFVNFSDYHIYTLNADKPKSRYIE"
BASE COUNT          293 a           53 c          107 g          235 t
ORIGIN      
        1 tttttttaca taaagggatc tcaaagaaat aaatagcatt tataatataa gtgtagtata
       61 aaagagtata ttagaaggag ttttaaaatt atgtcaaatg gttgtttgat agatgaatta
      121 aagaaattaa aagaatcatc aaaggaagca gtagaaaata tttcagattt tagtagtttt
      181 aaaaaatata tgcatgttaa aagacaagta gaaaacgaat tacttgatat tataaaatat
      241 acagctgatt ctaataaagc tgagttgata ttggtatgtg gtggagtagg agatggaaaa
      301 tcgcatttaa tatcatattt aaataacaaa tatccagaaa ttatgtcaaa atttataatt
      361 cataatgatg ctacagaaag ttttgaacca aataaaacat caatagatac acttaatgat
      421 gtacttgatg atttttcaga taagaaaatg gataattcag ataaaaagtt agtattagca
      481 ataaatttag gggcacttag taattttctt gaatcagaat atgttaataa atttactaaa
      541 ttaagagagt ttgttgaaaa taaaggaata cttgatatat ctatatcaga tgatgatgat
      601 aaaaattcga attttcattt tgttaatttt agtgattatc atatatatac tttgaatgca
      661 gataaaccta aatctagata tatagaat
//
LOCUS       DAEOSP010000389          687 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.394, whole genome shotgun sequence.
ACCESSION   DAEOSP010000389 DAEOSP010000000
VERSION     DAEOSP010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.394"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            100..507
                     /gene="tnpA"
                     /locus_tag="KRH54_003613"
     CDS             100..507
                     /gene="tnpA"
                     /locus_tag="KRH54_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBG8581236.1"
                     /translation="MEVTHGRGYVYLVQYHIVWCVKYRHPVLTNEIEEDLINILNKIA
                     EDNKFQILECNSDKDHIHLLINCSPQHYIPDIIKALKGVSARLLMKKHGEQLKKKLWG
                     GHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            509..>687
                     /locus_tag="KRH54_003614"
     CDS             509..>687
                     /locus_tag="KRH54_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8581237.1"
                     /translation="MAVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNNKETFTYKQCSSD"
BASE COUNT          297 a           64 c          114 g          212 t
ORIGIN      
        1 ttgcgtttct tacgtatgtt atttaaaaat aaatataaaa accttaagaa tacttgtttt
       61 aagtaattac tatgttaata ttatgttggg tgatgaaaaa tggaagtgac tcatggtaga
      121 gggtatgtgt atttagtaca ataccatata gtatggtgtg taaaatatag acatccagta
      181 ttaacaaatg aaattgaaga agatttaatt aatatattaa acaaaatagc tgaagataat
      241 aaatttcaaa tattagaatg taatagtgat aaagatcaca tacatttact aataaattgt
      301 tcaccacaac actatatacc agatataata aaagcattaa aaggtgtatc agcaaggctg
      361 ttaatgaaaa aacatggaga acagttgaaa aagaaattat ggggaggaca tctttggaat
      421 cctagttatt tcgttgctac tgtatcagaa aatacagaag aacagataag aaaatatatt
      481 aataatcaga aaaaacaagt aaagtgaggt ggcagtagtg gaaaaagctt ataagtttag
      541 aatgtatcca aataaaaaac aacaagagtt aattaacaaa acttttggat gttgtagatt
      601 tgtatataat aagtatcttg ctaaaagaat agaagtttat aaaaacaata aagaaacttt
      661 tacttataaa cagtgtagtt ctgattt
//
LOCUS       DAEOSP010000390          665 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.395, whole genome shotgun sequence.
ACCESSION   DAEOSP010000390 DAEOSP010000000
VERSION     DAEOSP010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.395"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            49..>665
                     /locus_tag="KRH54_003615"
     CDS             49..>665
                     /locus_tag="KRH54_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG8581238.1"
                     /translation="MTLKYKCYDMPLNTTLNYNQSTGAYEGTINYNQDPEYLNVWELQ
                     GITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIEDITSRKDVNKYLRKTSSPITE
                     LTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPILLVEKNNV
                     PNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNG"
BASE COUNT          295 a           93 c          106 g          171 t
ORIGIN      
        1 aaatcaaaag ttagtgtcaa atttaaagaa aaaccagatg cagatagcat tacattaaag
       61 tataaatgct atgacatgcc attgaataca actctaaatt acaatcaatc aactggggca
      121 tatgaaggaa ctatcaatta taaccaagac ccagaatatc taaatgtttg ggaactacaa
      181 gggataacaa taaacagcaa aaataatcct aaaactttaa acagacaaga cctagaaaag
      241 ctgggattaa atttaaaaga ctataatgta acacaggaat gtataattga agatataact
      301 tctagaaaag atgtaaataa atatttgaga aaaacttctt cacctattac agaacttaca
      361 ggaagtgata gatatgaaac agcagttaaa ataagtaaag agggctggaa aaatggttca
      421 gataaggtag ttataataaa tggggatgta agtatagatg gcattatatc aactccactg
      481 gcaaccacat ataatgcacc aatacttttg gttgaaaaaa acaatgtacc taatagtgta
      541 aaatcagaat taaagcgcct aaaccctaaa gatataatta taattggaga tgagaatgct
      601 atttctaaaa ctactgctaa tcaaattaaa tcaactgtaa atgctagtca aacacgttta
      661 aatgg
//
LOCUS       DAEOSP010000391          665 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.396, whole genome shotgun sequence.
ACCESSION   DAEOSP010000391 DAEOSP010000000
VERSION     DAEOSP010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.396"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            49..>665
                     /locus_tag="KRH54_003616"
     CDS             49..>665
                     /locus_tag="KRH54_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG8581239.1"
                     /translation="MTLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPEYLNVWELQ
                     GITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLRKTSAPITE
                     LTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPILLVEKNNV
                     PNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKG"
BASE COUNT          294 a           87 c          109 g          175 t
ORIGIN      
        1 acctcaaaag tcagtgttaa gtttaaagaa aaaccagatg cagatagcat tacattaaag
       61 tataaatgct atgatatgcc attggataca actttaaact ataatcaatc aactgagtca
      121 tatgaaggaa ctatcaatta taataaagac ccagaatatc taaatgtttg ggaactacaa
      181 gggataacaa taaacagcaa aaataatcct aaaactttaa acaaacaaga actagaaaag
      241 atgggattaa atttaaaaga ctataatgta acgcaggaat gtataattga agatataact
      301 tctagaaaag atgtaaataa atatttaaga aaaacttctg cacctattac agaacttaca
      361 ggaagtgata gatacgaaac agcagttaaa ataagtaaag aaggctggaa aaatggttca
      421 gataaggtag ttataataaa tggggatgta agtatagatg gcattatatc aactccacta
      481 gcaactacat ataatgcgcc aatacttttg gttgaaaaaa acaatgtacc taatagtgta
      541 aaatcagaat taaagcgttt aaatcctaga gatgtaatta taattggaga tgagaatgct
      601 atttctaaaa ctactgctaa tcaaattaaa tcgactgtaa atgctagtca aacacgtcta
      661 aaggg
//
LOCUS       DAEOSP010000392          664 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.397, whole genome shotgun sequence.
ACCESSION   DAEOSP010000392 DAEOSP010000000
VERSION     DAEOSP010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.397"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..>664)
                     /locus_tag="KRH54_003617"
     rRNA            complement(<1..>664)
                     /locus_tag="KRH54_003617"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          191 c          151 g          170 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 accacctgtc accaatgtcc ccgaagggaa ctctccgatt aaggagatgt cattgggatg
      541 tcaagcttag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc cgctacttgt
      601 gcgggtcccc gtcaattcct ttgagtttca ctcttgcgag cgtacttccc aggcggagta
      661 ctta
//
LOCUS       DAEOSP010000393          646 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.398, whole genome shotgun sequence.
ACCESSION   DAEOSP010000393 DAEOSP010000000
VERSION     DAEOSP010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.398"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(91..570)
                     /locus_tag="KRH54_003618"
     CDS             complement(91..570)
                     /locus_tag="KRH54_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBG8581240.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVVGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          259 a          104 c           49 g          234 t
ORIGIN      
        1 tatgtattat accctaatat attttatttt aaattactag ttagtcactc ttttcgggtt
       61 atttatctcc tatatattaa agtgtcacac ctaaatagtt aaatcattat cctcttttat
      121 ctttattgcc tttacaaatc catctgcaat tacaaaactc atcagagaag ttataaaata
      181 tacaaatact ggaacagtaa aataaaatcc aaatcctaaa tctagaaatc tcattccttt
      241 tccagtagta ccaaaagttg aaataaactc aattagagag tttaaaagca aaagatatcc
      301 aataatccta aatcttttta cattctcttt tacaaatagg ctatattcag aactacctaa
      361 tacttctatc aatgatttta taatgataaa atatgataca cctaaaataa acattaatat
      421 tccataaata gtaccttgta gagttgcttc tccactaaaa cagaggtaca taaaactttt
      481 aaatcctcct acaacaatta ctaatcctaa aagaaaaaat aaaatttgta atataccact
      541 tagtaaattt tttataagtt tattagacat catgccaact cctttaaaat attttatata
      601 tagtataata cattatatta tatcgttttt aaataaaaat attata
//
LOCUS       DAEOSP010000394          642 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.399, whole genome shotgun sequence.
ACCESSION   DAEOSP010000394 DAEOSP010000000
VERSION     DAEOSP010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.399"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            155..295
                     /locus_tag="KRH54_003619"
     CDS             155..295
                     /locus_tag="KRH54_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581241.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
BASE COUNT          273 a           62 c           90 g          217 t
ORIGIN      
        1 tataaaaaat aataataaag acgataagta ttaaaatttt atgtttccaa tatttatgat
       61 ataattaata tggcaaggaa taaatatttg aaaaagctat gagtggtgtt tccatggaag
      121 tttcttcact ttcttatgag aggaggtgaa aactatggaa aatctaatga tgagtattat
      181 aactggtgtt atagccagtt acatttacga taaattaaag agccactctg agcgaccaac
      241 taagagtggc tgggaactta atataaagtt ccagaaaagt aaatcttcaa aataatttgt
      301 tgaaggaaac atcactcaaa gttaagtaaa atatgtttcc ttgcttttat tatatcacaa
      361 attaaaaata ttaacacaaa attttgtgaa tatatactta ttattgttct aaaaaaatat
      421 gtaaataatt gatttgattg catggaatta ttgatgtaat atttaagtta aatatacaca
      481 atctcaatta aagaagataa gtactataaa ttcatatcaa aaaaatacat aaacttttaa
      541 gttataattt tataaaaagt cttttagtat cattatttca agaggaatta tcatatgaaa
      601 aaataacttc attagaaaag tggataaaaa actggtaaga ag
//
LOCUS       DAEOSP010000395          630 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.400, whole genome shotgun sequence.
ACCESSION   DAEOSP010000395 DAEOSP010000000
VERSION     DAEOSP010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.400"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            28..>630
                     /locus_tag="KRH54_003620"
     CDS             28..>630
                     /locus_tag="KRH54_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBG8581242.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSNI"
BASE COUNT          219 a           55 c           84 g          272 t
ORIGIN      
        1 aatttaatca tttttgagga tattattatg ttagatgcat ttataacaat tgtttcttct
       61 attatagaaa tatggtcatg taaaaaagtt tttgattata ctagtcagtc aaagaccgac
      121 ttaaaaaaac taaatatgta ttttctcttt tctgtttttc tcattatttc ttctttatat
      181 ataaatatag gagctaatag tagaatcttg atatgtatat ttgtaatgtt cgttttctat
      241 aagtctaact ataaggttaa tatatataaa tgtctggtaa tctcattttt ttattggtta
      301 atattaatag gtataaatgg attaagtatg ttattagtag caacagtaag caatttggat
      361 aatatgaatg ttttacttag tggcagttta tatagatatg aagcaatatt cttgggaaag
      421 ataatgttag gtatattact ttatgtttat aaggcttctt ttaaaaattt taaaataagt
      481 aaaaaagaga tatacatatg tattcctatg atagcaaatt tattgtcatt ttttgtactt
      541 tatgattata ttattaatct tgtaaaaatc tctcctgaaa gaagaactgc ttttttagta
      601 atagttttac tattaatatt atctaacatt
//
LOCUS       DAEOSP010000396          612 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.401, whole genome shotgun sequence.
ACCESSION   DAEOSP010000396 DAEOSP010000000
VERSION     DAEOSP010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.401"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..>612
                     /locus_tag="KRH54_003621"
     CDS             <1..>612
                     /locus_tag="KRH54_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG8581243.1"
                     /translation="IKLPKLGMVKVRDKQVPQGRILNATISKEPSGRYYVSLCCTNVD
                     IEVFENTNNQIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQRELSRKTIGSLN
                     RNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLIS
                     DVSWSEFVRQLKYKANWHGRQIVKVGKFFASSQICNKCGYKNE"
BASE COUNT          254 a           67 c          105 g          186 t
ORIGIN      
        1 atataaaact acctaaactt ggtatggtta aggtaaggga taaacaagta cctcaaggta
       61 gaatacttaa tgctactata tctaaagaac caagtggtag atattatgta tcattatgct
      121 gtactaatgt agatattgaa gtatttgaaa atactaataa tcagataggt ttagatttag
      181 gaattaaaga attttgtatc tcaagttgtg gagactttat agaaaatcct aagtatctta
      241 agaagtcatt aaacaaactt gctaaattac aaagagaatt atcgagaaag acaatcggta
      301 gtttaaatag aaataaagca agattaaaag ttgcaagact tcaagaacat atagctaatc
      361 aaagaaatga ttttctacaa aaactatcta ctaaattaat aaaagagaat gatattattt
      421 gcatagaaga tttacaagta aagaatatga ttagaaatcg taaactatct cgcttaattt
      481 ctgatgtgtc atggtcagaa tttgttcgtc aattaaaata caaggctaat tggcatggta
      541 gacaaattgt aaaggtaggt aaattctttg caagttcaca aatatgcaat aaatgtgggt
      601 ataaaaacga gg
//
LOCUS       DAEOSP010000397          599 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.402, whole genome shotgun sequence.
ACCESSION   DAEOSP010000397 DAEOSP010000000
VERSION     DAEOSP010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.402"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(269..493)
                     /gene="secG"
                     /locus_tag="KRH54_003622"
     CDS             complement(269..493)
                     /gene="secG"
                     /locus_tag="KRH54_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBG8581244.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
BASE COUNT          225 a           97 c           60 g          217 t
ORIGIN      
        1 ctcattaaac atggtgaaaa tagtgttgct atatattttt taatttgaaa aaaatatata
       61 aatacaaaca atatataaat ataaataata aacgccaaca aatagtcaat ctataaaata
      121 aaaactggac taatcatata aaatcattag tccagctttt attttacgta ctttttatgt
      181 tgatgtactt ttattaaaaa ctaaatttaa tttttaaatg tctagcaaat gcactaatta
      241 aataatggcc agttattcaa caatatcact acttcttaac tataagtaat gccaatgcat
      301 ttataaaaaa taatactgct gttatctttg taactctagc aagaaatgct tgttttcctt
      361 ttggtttaaa gtaagcttgg cttccttctc caccaaattc tgaaggtaaa gcattcttag
      421 aatcttgagg cattatgcta attactagta aaatccctag tactacttgc acacccatta
      481 aaatgttgct cattcttgcc cctcctatct gtttattact taaatttcat tactctttag
      541 tgtatcacaa taatatagcc atttcaagac tattatgcta taaataacat acaaaaaaa
//
LOCUS       DAEOSP010000398          577 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.403, whole genome shotgun sequence.
ACCESSION   DAEOSP010000398 DAEOSP010000000
VERSION     DAEOSP010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.403"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..>577
                     /locus_tag="KRH54_003623"
     rRNA            <1..>577
                     /locus_tag="KRH54_003623"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DAEOSP010000399          573 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.404, whole genome shotgun sequence.
ACCESSION   DAEOSP010000399 DAEOSP010000000
VERSION     DAEOSP010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.404"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..>573
                     /gene="tuf"
                     /locus_tag="KRH54_003624"
     CDS             <1..>573
                     /gene="tuf"
                     /locus_tag="KRH54_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBG8581245.1"
                     /translation="IPAPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLKVQDEV
                     ELVGLTEAPRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTGSVK
                     AHTKFTAEVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVT
                     MEVDLINSIVVEEGLRFSIREGGRTVASGV"
BASE COUNT          242 a           77 c          140 g          114 t
ORIGIN      
        1 atataccagc tccagagaga gatacagata aaccattctt aatgccagta gaggacgtat
       61 tctcaatcac aggaagagga acagttgcaa caggaagagt ggaaagagga gtactaaaag
      121 tacaagacga agtagaatta gtaggattaa cagaagcacc aagaaaagta gtagtaacag
      181 gagtagagat gttcagaaaa ttattagacc aagcacaagc aggtgataat ataggagcat
      241 tattaagagg agtacaaaga aacgagatag aaagaggaca agtactagca aagactggat
      301 cagtaaaggc acacacaaag tttacagcag aagtatatgt acttaaaaaa gaagagggtg
      361 gaagacatac accattcttt gatggatata gaccacaatt ctatttcaga acaacagacg
      421 taacaggagc ttgtaagtta ccagaaggaa tagagatggt aatgcctgga gataacgtaa
      481 caatggaagt agacttaata aactcaatag ttgtagaaga gggattaaga ttctcaataa
      541 gagaaggtgg aagaacagta gcttcaggag ttg
//
LOCUS       DAEOSP010000400          573 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.405, whole genome shotgun sequence.
ACCESSION   DAEOSP010000400 DAEOSP010000000
VERSION     DAEOSP010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.405"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
BASE COUNT          226 a           53 c           52 g          242 t
ORIGIN      
        1 gcctattaat ttaatgcaat aaataataaa tcttttacaa aaagataata aaaatataga
       61 aaaatatttt ttattgtcct tttggttatg gtaaaccaca ttctgcctgc aatataacta
      121 aatatatggg tagatagtaa aattaatttt attatttata tataatttta taagttactt
      181 ttcaataata ttgactgtca ttaatttata atattcaata aaatatttta atttagttaa
      241 gaatctatat tgtaaaggag tattaaatta atgaacttaa aattaatatg gataaatatt
      301 ctaaacccag tatttattaa aaatgaaata aaaagaaaaa aaatacaaat tattatcttt
      361 ttctttataa actttggttt aacttattca ttaggtttac tgttatatta taataaattt
      421 attgatccag aaaactttgc atcattcatg atgatattac ctctatcttc agttgccatt
      481 gcaaagtttt atactgaagg attaaataat gatatgtatg aattttattc actaatcata
      541 ttattttttc ttatttattc attattaatt gtt
//
LOCUS       DAEOSP010000401          541 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.406, whole genome shotgun sequence.
ACCESSION   DAEOSP010000401 DAEOSP010000000
VERSION     DAEOSP010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.406"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
BASE COUNT          215 a           43 c           64 g          219 t
ORIGIN      
        1 aaatttatat aaaaaagaat atgagttgta aaatattaaa taatattgta ctaattggaa
       61 taattttaac tttattatag attattttaa taccattagt actgatggtg tattttaaaa
      121 ttggtataag cattacaaat acgaatatgc caactgtaat atgtaatata agtagctata
      181 tacatatgtg ctgtcccata tattgtatca ttgtttatat taaaaagatt atgtaaattg
      241 gtatcaatca ataaatcatt ttcagaagaa atacctaaaa acttaaaaaa tacctatatg
      301 tgtatttagt tagatttgag atttttacat ttaattttat agtgacaata ttatattatt
      361 tttatgatgt ctatttatat gcaattacta taatatcaga acttatagta tcatttgttt
      421 cattatcaat aaggtttttt actttagttt tgtcaatttt attaaaaaaa gttattgata
      481 taaaagataa gaaggataaa actatatgac ggatttcaaa cataagagtg gatatttata
      541 t
//
LOCUS       DAEOSP010000402          539 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.407, whole genome shotgun sequence.
ACCESSION   DAEOSP010000402 DAEOSP010000000
VERSION     DAEOSP010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.407"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
BASE COUNT          241 a           14 c          114 g          170 t
ORIGIN      
        1 atataattaa tgaaaaaatt taaactttgt aatatataaa taaaaaaact ctaaaataga
       61 aggatataat tatatagaat gtttcaatta aacaaggtaa aaactataat cttaaaatta
      121 aaattaacat tattcttaag aagcaagaaa tttagaaagt aggaagttta gtgataagtg
      181 gtagtaagta agaaatttag gaagtgggaa gtttagtgat aagtggtagt aagtaagaaa
      241 tttaggaagt gggaagttta gttataagtg gtagtaagta agaaatttag gaagtgggaa
      301 gtttagttat aagtagtagt aagtaagaaa cttaggaagt gggaagttta ttagtaataa
      361 tttatagcaa gtaagaaatt tagtaataat ttgtagcaag taagaaattt agaaagtagg
      421 aagtttagta ataatttata gcaagtaaga aatttaggga gtaggaagtt tagtaataat
      481 ttgtggtaag taagaaattt agaaagtagg aagtttagta ataatttata gtaagtaag
//
LOCUS       DAEOSP010000403          534 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.408, whole genome shotgun sequence.
ACCESSION   DAEOSP010000403 DAEOSP010000000
VERSION     DAEOSP010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.408"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            83..451
                     /locus_tag="KRH54_003625"
     CDS             83..451
                     /locus_tag="KRH54_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBG8581246.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT          224 a           49 c           95 g          166 t
ORIGIN      
        1 aaatagtata tatgatatgt agaccattag catgtttaaa gtagcaaaac atataaaata
       61 aaatttgaaa ggaagtataa aaatgacaag accatcaata tatcatagcc aaggatacac
      121 ttgtgcagag gcattaataa agtcttataa tgaggaacac aacacagata taccaatctc
      181 aataggaagt ggtatggggg ttggaatgaa tgttggaagt gtttgtggag ctgtaaatgc
      241 tgctgttgtt ataatagggt atttaaatgg gcgaaatagt aatttagatg aaaatatagc
      301 aaggaagtat tcaagagagc tattgaatag agtaagaaat aaatacagct cagaaatatg
      361 tgtatctctt aaaaaaaata aagtaacttg tgaagaaata ataaatttca catatgatgc
      421 tctaaatgat atgctaaaaa atcaaaaata attctaagaa acaagtattt tacattcgat
      481 agaatacttg tttttttatg atatttttaa taagtattag tttattataa tttt
//
LOCUS       DAEOSP010000404          498 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.410, whole genome shotgun sequence.
ACCESSION   DAEOSP010000404 DAEOSP010000000
VERSION     DAEOSP010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.410"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(132..431)
                     /locus_tag="KRH54_003626"
     CDS             complement(132..431)
                     /locus_tag="KRH54_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581247.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
BASE COUNT          187 a           69 c           51 g          191 t
ORIGIN      
        1 tttacaaatt gttaagtaat attttatgat ataaatcaga agaggcactt ctataataga
       61 aagtgctcaa ctatgaataa gattaataat atatttgcct tagatatata gacttttata
      121 aatattaaag attaatcaaa tctgatgttt gaaactttat cccaattaat cttccttgaa
      181 tagtcttctg gatgtgataa tctaactata tgagttgaat tatcatagct gtaataaatt
      241 gtagtagtac catcaactgg attctttttg tatccttgat acacagcact atttggtact
      301 ttcttttcaa caaagctata tgcagatgaa gtatcaatag tttctatttt attaggaaat
      361 ataaacatat aaaaagctac tactataaaa atacatatgc atgtaatact ataccattta
      421 atccatttca taaaattcct cctactttac caaaatatac tattattatt tccatatatt
      481 tataatattt atacatac
//
LOCUS       DAEOSP010000405          493 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.411, whole genome shotgun sequence.
ACCESSION   DAEOSP010000405 DAEOSP010000000
VERSION     DAEOSP010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.411"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            188..304
                     /gene="rrf"
                     /locus_tag="KRH54_003627"
     rRNA            188..304
                     /gene="rrf"
                     /locus_tag="KRH54_003627"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            310..398
                     /locus_tag="KRH54_003628"
     tRNA            310..398
                     /locus_tag="KRH54_003628"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Leu,seq:taa)
     gene            410..486
                     /locus_tag="KRH54_003629"
     tRNA            410..486
                     /locus_tag="KRH54_003629"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:444..446,aa:Ile,seq:cat)
BASE COUNT          156 a           91 c          109 g          137 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgctaactc tatattttta aacttgtcaa
       61 aacttatgaa tttttaatag ttttataagg caaaaaatcg aataaaaaaa gtattgacac
      121 aataagacaa agatgttatt ataatacttg tcttagaaaa aaagttaaat gaaaatttag
      181 acaataatct ggttataata gcagagagga tacacctgtt cccattccga acacagaagt
      241 taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta ggacatagcc
      301 aggtgatgtg ccgaagtggc ggaactggca gacgcacagg acttaaaatc ctgcgggact
      361 tacctctcgt accggttcga ttccggtctt cggcaccaca aattattgtg ggccattagc
      421 tcagttggtt agagcgcccg gctcataacc ggtaggtctg gggttcgagt ccctgatggc
      481 ccaccacaat aat
//
LOCUS       DAEOSP010000406          482 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.412, whole genome shotgun sequence.
ACCESSION   DAEOSP010000406 DAEOSP010000000
VERSION     DAEOSP010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.412"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            3..79
                     /locus_tag="KRH54_003630"
     tRNA            3..79
                     /locus_tag="KRH54_003630"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Asp,seq:gtc)
     gene            90..164
                     /locus_tag="KRH54_003631"
     tRNA            90..164
                     /locus_tag="KRH54_003631"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:122..124,aa:Gly,seq:gcc)
     gene            174..247
                     /locus_tag="KRH54_003632"
     tRNA            174..247
                     /locus_tag="KRH54_003632"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:208..210,aa:Arg,seq:tct)
BASE COUNT          150 a           83 c          102 g          147 t
ORIGIN      
        1 gcggcctggt agttcagttg gttagaatgc cagcctgtca cgctggaggt cgagggttcg
       61 agccccttcc aggtcgccaa attaaataag cgggaatagt tcagtggtag agcgcaacct
      121 tgccaaggtt gaagtcgcga gttcgaatct cgtttcccgc tccataataa cttgggtgca
      181 tagctcagct ggatagagca acgcccttct aaggcgtgtg tccggggttc gaatccctgt
      241 gcgctcatta agaaaagttt actataaatt atagtaaact tttatattgt ttaaaaagac
      301 acaggaaaca tgtatattac ctgtgtcttt ttatttataa actttaaact atgtaaaaat
      361 tacagtatat tctaatagag ttacagtata ttcattaata ttgtaaacat acggagggta
      421 atgtataata gtctacaaga tgtttatgaa ggcatcagtt aaatataata aatataacta
      481 at
//
LOCUS       DAEOSP010000407          475 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.413, whole genome shotgun sequence.
ACCESSION   DAEOSP010000407 DAEOSP010000000
VERSION     DAEOSP010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.413"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(139..339)
                     /locus_tag="KRH54_003633"
     CDS             complement(139..339)
                     /locus_tag="KRH54_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBG8581248.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT          184 a           71 c           39 g          181 t
ORIGIN      
        1 attttaaaaa agttagttta agtttgacgc ttttctaaag taacttctta tttttgtaat
       61 taaaaataaa tataataaac aacaaaaaag agccctcgat agctcctttt ctaaaatata
      121 tatatttaaa ttaaataatt atattctatt tacgttttca gcttgaggtc ctctattacc
      181 ttgagttata tcaaagctaa ccttttcgcc ttcttctaaa gttttgtatc catcattttg
      241 tatagctgag aaatgtacga aaacatcatc ttctccctct acagatatga acccaaatcc
      301 tttttcattg ttaaaccatt ttactattcc gtttttcatt ataagaaaac ctccgcatta
      361 ttaaaaaata caaactaaaa aaataagtct gttctattaa tataacataa attttaaata
      421 aaagcaaata aaatttttaa aaaaattaat tacttttaga atttaataaa aattg
//
LOCUS       DAEOSP010000408          457 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.414, whole genome shotgun sequence.
ACCESSION   DAEOSP010000408 DAEOSP010000000
VERSION     DAEOSP010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.414"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            46..327
                     /locus_tag="KRH54_003634"
     CDS             46..327
                     /locus_tag="KRH54_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581249.1"
                     /translation="MASKQIPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
BASE COUNT          197 a           29 c           73 g          158 t
ORIGIN      
        1 aaaatttatt attaaataaa aaataaagga gagtgattta atagcatggc atcaaaacaa
       61 ataccaagaa acaatttaga aaagttttta agaataagag tcttatcaca tacaggcgat
      121 aaaatacata taaagttacc tgtaaatttt gcaaagagaa tgattgagaa taatgctttg
      181 gatttattta atggaaaaga tgatgtagta gatggtaaaa aagttactga aatattagtt
      241 aaggcatttg actatgattt aactggagaa gtagttcact tggagagaaa aaatggagac
      301 attattaaag tttcaattgt acaatagaat tattttatat gaattttatt tttgaatagt
      361 tataaataat tagaaatttg taaaaaagta taatatatta ttagtaatgg tacagaatac
      421 acatttataa tttatagatg tgtatttttt gtttaaa
//
LOCUS       DAEOSP010000409          437 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.415, whole genome shotgun sequence.
ACCESSION   DAEOSP010000409 DAEOSP010000000
VERSION     DAEOSP010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.415"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..403)
                     /locus_tag="KRH54_003635"
     CDS             complement(<1..403)
                     /locus_tag="KRH54_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HBG8581250.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLV"
BASE COUNT          148 a           56 c           42 g          191 t
ORIGIN      
        1 ctactaatgg tttcatatta ttattataaa tacttatatc tgcattactt aattccattt
       61 ttatgcgatt aataagattt tctttatact ttgtaatatt aggtatggat ttaataaaca
      121 ttcttgatac agaaggtttt ttatctacga tatgacttag tatactatct atatttattt
      181 caattttatt atcaatatcc cactgagcat ttgcagagat taataacaat ccccttgttc
      241 gaatatatga gttttcgtta tcaatcattt ctaggaatga gtcaaaaaaa caatatgtaa
      301 tatttgattt taaactttca ttttctaaga ataaaaaagt tttatatgct tcattatgat
      361 tttttgaata taatttatta ataagtaaat tgagattctt caatcttctc atctccctca
      421 ttacaaatat ttattta
//
LOCUS       DAEOSP010000410          434 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.416, whole genome shotgun sequence.
ACCESSION   DAEOSP010000410 DAEOSP010000000
VERSION     DAEOSP010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.416"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            <1..>434
                     /locus_tag="KRH54_003636"
     CDS             <1..>434
                     /locus_tag="KRH54_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBG8581251.1"
                     /translation="LLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIIL
                     TDKNSITDNTYKWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRH
                     ETNANVIKKFYTDDELEAVLVAKSDVLVDALAAGPLAANLKSP"
BASE COUNT          182 a           55 c           82 g          115 t
ORIGIN      
        1 ttattgatag caaaggaaat agataaaaat catgatgtgg aaaaagtata cataacaaat
       61 gctaatggcg gagaagtgga tgcacttact atagcagcaa aagcaggtca agacaagcaa
      121 ccaattatat taactgataa aaatagtatt acagacaaca catataaatg gttaaagagt
      181 gaggatttac aaaatgctta ttttataggt ggccctcaaa tgatatcaac aaatgttata
      241 aataaggtaa atgatataac taaagataat gttactaata atagagtata tggagcagat
      301 agacacgaaa caaatgcaaa cgtaataaaa aaattctata cagatgatga gttagaggct
      361 gttttagtag ctaaatcaga tgtacttgtt gatgctttag cagcaggtcc attggctgcg
      421 aacttaaaat ctcc
//
LOCUS       DAEOSP010000411          415 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.417, whole genome shotgun sequence.
ACCESSION   DAEOSP010000411 DAEOSP010000000
VERSION     DAEOSP010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.417"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..>415)
                     /locus_tag="KRH54_003637"
     CDS             complement(<1..>415)
                     /locus_tag="KRH54_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBG8581252.1"
                     /translation="RILNATISKEPSGRYYVSLCCTDVDIEAFENTNNHIGLDLGIKE
                     FCISSCGEFIENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKARLEVAGLQEHIANQR
                     KDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSR"
BASE COUNT          128 a           67 c           43 g          177 t
ORIGIN      
        1 agcgagatag tttacgattt ctaatcatat tctttacttg taaatcttct atgcaaataa
       61 tatcattctc ttttattaat ttagtagata gtttttgtaa aaaatccttt ctttgattag
      121 ctatatgttc ttgaagtcct gcaacttcta atcttgcttt atttctattt aaactaccga
      181 ttgtttttct cgataattct ctttgtaatt tagcaagctt gtttaatgac ttcttaagat
      241 acttaggatt ttctataaat tctccacaac ttgagataca aaattcttta attcctaaat
      301 ctaaacctat atgattatta gtattttcaa atgcttcaat atctacatca gtacagcata
      361 atgatacata atatctacca cttggctctt tagatatagt agcattaagt attct
//
LOCUS       DAEOSP010000412          411 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.418, whole genome shotgun sequence.
ACCESSION   DAEOSP010000412 DAEOSP010000000
VERSION     DAEOSP010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.418"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
BASE COUNT          173 a           46 c           32 g          160 t
ORIGIN      
        1 aaatgttaag ataaaatttt tagatttata tcacaattta gaattatttg taaaaaagga
       61 gattatcttt ttattagaca atccccttgt atattttatt aatatttatc tatgattaag
      121 catcaatttt attttatact tttttatttt aatactatac atctaaatct tatttagggg
      181 gaacaattat ttatatgcta taaaaatgct aatttgaacg aacttgtctt ataatcagtt
      241 ttagaaaata caccattcta caaagtatga atcaaaaggt gaaaaaatac taaaaataat
      301 aaatcaacat caattttaat caatatatga aaaacttata ccaagtatgc ataaaaacta
      361 atataaatat tattttaaca actaatactt acacctttat atattatatc a
//
LOCUS       DAEOSP010000413          408 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.419, whole genome shotgun sequence.
ACCESSION   DAEOSP010000413 DAEOSP010000000
VERSION     DAEOSP010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.419"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            15..105
                     /locus_tag="KRH54_003638"
     tRNA            15..105
                     /locus_tag="KRH54_003638"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49..51,aa:Ser,seq:gct)
     gene            114..190
                     /locus_tag="KRH54_003639"
     tRNA            114..190
                     /locus_tag="KRH54_003639"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:148..150,aa:Pro,seq:tgg)
     gene            276..351
                     /locus_tag="KRH54_003640"
     tRNA            276..351
                     /locus_tag="KRH54_003640"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:309..311,aa:Trp,seq:cca)
BASE COUNT          104 a           85 c          114 g          105 t
ORIGIN      
        1 aaatgactga atttggagaa gtactcaagt ggctcaagag gatcccctgc taagggatta
       61 ggctgggtaa ccggtgcgag ggttcgaatc cctccttctc cgccattcaa aaacgaggtg
      121 tagcgcagtt tggtagcgca catggtttgg gaccatgggg ccgggggttc gagtcccttc
      181 acctcgacca acttaagctt ttagttcagt tggttagagt gcccaactca tggctagtaa
      241 ttctagggtt tgagtccatg aaggttattc ttcatagggg tgtagctcag ttggtagagc
      301 gtcggtctcc aaaaccgtgc gccgggggtt cgagtccctc cacccctgcc aaataaaaag
      361 gacttgaaaa aagttggatt ttagagtagt ataagatagt aaataaga
//
LOCUS       DAEOSP010000414          404 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.420, whole genome shotgun sequence.
ACCESSION   DAEOSP010000414 DAEOSP010000000
VERSION     DAEOSP010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.420"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            54..275
                     /locus_tag="KRH54_003641"
     CDS             54..275
                     /locus_tag="KRH54_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581253.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT          187 a           23 c           76 g          118 t
ORIGIN      
        1 ctttgggaaa aataaatata ggaagtatta tatttacaga ggaggtagtt ttgatggata
       61 gattaaaaag agatatgaaa aatatgaaag aagatttaaa agaaggtgtt aaaaagacag
      121 cttatgttat gaaaaataca gctgaagatt tgaaagatga tgcacaaggt gctatggtaa
      181 acttagaaat gaaaaaagat gagatgatgg aaagatatga acaaaagaaa ttttctaaag
      241 aattagagaa gcaaatgaag aatgaaatga aataagcttt tatttatgaa taacaaatta
      301 ttgtattcat ataaaaagta gccagtaata ttaaatagtt tatttaagga aatagacttt
      361 ctaaaaataa ctgtattaga tttagaaagt ctatttcaaa ttag
//
LOCUS       DAEOSP010000415          389 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.421, whole genome shotgun sequence.
ACCESSION   DAEOSP010000415 DAEOSP010000000
VERSION     DAEOSP010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.421"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
BASE COUNT          160 a           48 c           31 g          150 t
ORIGIN      
        1 ttctctaagt ttatatttaa atcagcgtta ttttccatag cattagattc agtagcaaat
       61 gccattgtag gtgcgacgat tgccattgaa agaatccctg ttgttaataa ttttgtattt
      121 cttctcatat taatctcctt atttaacata aatttctact ttgtttttta accttttaat
      181 ttttgaaatt atatcaaagt gtttttaccc aaaattataa ataagtacat aaaaaataaa
      241 cttctaaaaa aattataaaa acaaaaaata aaaaaattaa atatattgaa tatatttaat
      301 tatcttcact atatcattta ttgccaaaaa aatctataaa agacaaataa aaacatataa
      361 caattgtagg tttattaaat ttaatacaa
//
LOCUS       DAEOSP010000416          383 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.422, whole genome shotgun sequence.
ACCESSION   DAEOSP010000416 DAEOSP010000000
VERSION     DAEOSP010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.422"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
BASE COUNT          180 a           25 c           38 g          140 t
ORIGIN      
        1 taattatcta attatattta aggataataa tatgaaaata atttttatat taaaaaatat
       61 gaatgaaaat agtgggtttt ataaaaaaac atatttagat tttaacatct caaaggagtt
      121 ttcaaagcta atatttcatt tttaaataaa aaaataaaat attagttttg atttctggtt
      181 taaattgggt tataataaat tgtcaaatta tttattaata taaaatagac tataaaacaa
      241 gcaaaaatca tactattata taaaagacct taaagataga aaactacaga aagaagccaa
      301 aatgtttttg aaaaaaatga aattttattg caaaaaaatc aaaatgaatt taacttaata
      361 gttataatct atttttagtg tta
//
LOCUS       DAEOSP010000417          364 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.423, whole genome shotgun sequence.
ACCESSION   DAEOSP010000417 DAEOSP010000000
VERSION     DAEOSP010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.423"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            168..284
                     /gene="rrf"
                     /locus_tag="KRH54_003642"
     rRNA            168..284
                     /gene="rrf"
                     /locus_tag="KRH54_003642"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            291..362
                     /locus_tag="KRH54_003643"
     tRNA            291..362
                     /locus_tag="KRH54_003643"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:323..325,aa:Asn,seq:gtt)
BASE COUNT          117 a           61 c           78 g          108 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc tatgtaaagt actaacttca
       61 attgatgaag tatatatcgt aaaaacaatt actatttagt acttcaaaac tattgacatt
      121 taaaaataaa gatgttaata taatacttgt cttataaata ggcaatatgt ggttataata
      181 gcaaagagga tacacctgtt cccattccga acacagaagt taagctcttt agcgctgatg
      241 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtgaatat tccaaggtag
      301 ctcaatggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc ccacccttgg
      361 agcc
//
LOCUS       DAEOSP010000418          341 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.424, whole genome shotgun sequence.
ACCESSION   DAEOSP010000418 DAEOSP010000000
VERSION     DAEOSP010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.424"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            12..88
                     /locus_tag="KRH54_003644"
     tRNA            12..88
                     /locus_tag="KRH54_003644"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46..48,aa:Pro,seq:tgg)
     gene            96..172
                     /locus_tag="KRH54_003645"
     tRNA            96..172
                     /locus_tag="KRH54_003645"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:130..132,aa:His,seq:gtg)
     gene            181..256
                     /locus_tag="KRH54_003646"
     tRNA            181..256
                     /locus_tag="KRH54_003646"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:214..216,aa:Lys,seq:ttt)
     gene            264..337
                     /locus_tag="KRH54_003647"
     tRNA            264..337
                     /locus_tag="KRH54_003647"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:296..298,aa:Cys,seq:gca)
BASE COUNT           78 a           79 c           96 g           88 t
ORIGIN      
        1 taatataaat tcgaggtgta gcgcagtttg gtagcgcaca tggtttggga ccatggggcc
       61 gggggttcga gtcccttcac ctcgaccaat ataaggtggg tatagctcag ttggttagag
      121 cgccagattg tggctctgga ggtcgtgagt tcgactctca ttatccaccc caattcatat
      181 ggtctattag ctcagtcggt agagcacctg acttttaatc agggtgtccc gcgttcgagt
      241 cgcggataga tcaccaaaaa ggtggcgaca tagccaagtg gtaaggcagt ggactgcaac
      301 tccttgatcc ccagttcgaa tctgggtgtc gcctccaaaa t
//
LOCUS       DAEOSP010000419          332 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.425, whole genome shotgun sequence.
ACCESSION   DAEOSP010000419 DAEOSP010000000
VERSION     DAEOSP010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.425"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
BASE COUNT          147 a           31 c           34 g          120 t
ORIGIN      
        1 cattaaatat gccaatagtt ttatatatta ttgattttat taaaaaaaga gatatacaaa
       61 ctaataagtt gtaatctctc aaatataaaa aaacaataaa ttagctcatt ggatttttta
      121 gtccataata taattataga ttattcacca aacaatctat agaaattgtg gtattaatgt
      181 ttaaataacc aactttttct aaaataacct ttataggaat atatgagttc ataagtaaag
      241 atagcgaaat aagaaaccag tttggtatgt aaaatctaga tttttaatta aattatcaag
      301 attatatatt atagtatcaa aaatagaaaa tt
//
LOCUS       DAEOSP010000420          312 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.426, whole genome shotgun sequence.
ACCESSION   DAEOSP010000420 DAEOSP010000000
VERSION     DAEOSP010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.426"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
BASE COUNT          124 a           30 c           27 g          131 t
ORIGIN      
        1 tttaaataac tgtagattta ttaaagttat ctatgtacta tattaaacaa ttaagtagtt
       61 attaattgca cttattagtt tattaaaaaa ctatgtatat tactttgcag taagactttg
      121 gaactaaatc tataatagca aatttaaatt tattaaatag attaccttat aacatttcat
      181 ataaaatata ttgtgaatct gagaaaatta ctatttttct ttgagtattt tttgtaagca
      241 gttctaaaag attcaatttc atacctatta cttgaatcat tcattaaaat aattctaatt
      301 taaaaaataa tt
//
LOCUS       DAEOSP010000421          257 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.427, whole genome shotgun sequence.
ACCESSION   DAEOSP010000421 DAEOSP010000000
VERSION     DAEOSP010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.427"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            5..81
                     /locus_tag="KRH54_003648"
     tRNA            5..81
                     /locus_tag="KRH54_003648"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Ile,seq:gat)
     gene            85..160
                     /locus_tag="KRH54_003649"
     tRNA            85..160
                     /locus_tag="KRH54_003649"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:118..120,aa:Phe,seq:gaa)
     gene            168..244
                     /locus_tag="KRH54_003650"
     tRNA            168..244
                     /locus_tag="KRH54_003650"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Met,seq:cat)
BASE COUNT           59 a           66 c           72 g           60 t
ORIGIN      
        1 aaaagggcct atagctcagg tggttagagc gcacgcctga taagcgtgag gtcgctggtt
       61 cgagtccagc taggcccacc atttgcccag atagctcagt cggtagagca ggggactgaa
      121 aatccccgtg tcggtggttc gattccgcct ctgggcacca ggaatacggc ggtatagctt
      181 agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc tattaccgct
      241 accatctaac tatatca
//
LOCUS       DAEOSP010000422          235 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.428, whole genome shotgun sequence.
ACCESSION   DAEOSP010000422 DAEOSP010000000
VERSION     DAEOSP010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.428"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            119..235
                     /gene="rrf"
                     /locus_tag="KRH54_003651"
     rRNA            119..235
                     /gene="rrf"
                     /locus_tag="KRH54_003651"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT           92 a           32 c           46 g           65 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc taaaaaaaaa taaaaaaagt
       61 attgacaaaa atagtaaaaa atgttaatat tatacttgtc ctagcaacaa gaaaaaaatg
      121 tggttataat agcggagaag atacacctgt ttccattccg aacacagaag ttaagttctc
      181 tagcactgat ggtacttggt gggaaactgc ctgggagagt aggacatagc cacgt
//
LOCUS       DAEOSP010000423          232 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.429, whole genome shotgun sequence.
ACCESSION   DAEOSP010000423 DAEOSP010000000
VERSION     DAEOSP010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.429"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..>232)
                     /locus_tag="KRH54_003652"
     CDS             complement(<1..>232)
                     /locus_tag="KRH54_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HBG8581254.1"
                     /translation="NVIQKIQQLVPPTHPVLGDGILVLTDIKSSPYPGASVVPDYCKA
                     TFDRRLLVGETREGVLAPIQALLDEMMKEDPE"
BASE COUNT           66 a           41 c           37 g           88 t
ORIGIN      
        1 aattctgggt cttctttcat catttcatct aataaagctt gtattggagc taatacgcct
       61 tctcttgttt ctccaactaa taatcttcta tcaaaagtag ctttgcaata atctggcact
      121 actgatgctc ctggatatgg agaagatttt atatctgtta aaactaatat tccatcacct
      181 aaaactggat gagttggagg tactaattgt tgtatctttt ggattacatt ag
//
LOCUS       DAEOSP010000424          206 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.denovo.430, whole genome shotgun sequence.
ACCESSION   DAEOSP010000424 DAEOSP010000000
VERSION     DAEOSP010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.denovo.430"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..167)
                     /locus_tag="KRH54_003653"
     rRNA            complement(<1..167)
                     /locus_tag="KRH54_003653"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           52 a           55 c           39 g           60 t
ORIGIN      
        1 ttatcccgta ttagcatacc tttcggtatg ttatccgtgt gtacagggta ggttacccac
       61 gcgttactca cccgtccgcc gctctttacc gaagtaaatc gctcaacttg catgtgttag
      121 gcacgccgcc agcgttcatc ctgagccagg atcaaactct caaataaaag tttatcaggc
      181 tcagatacta ttgttatcaa aatatc
//
LOCUS       DAEOSP010000425        12816 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.guided.115, whole genome shotgun sequence.
ACCESSION   DAEOSP010000425 DAEOSP010000000
VERSION     DAEOSP010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.guided.115"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            477..1178
                     /locus_tag="KRH54_003654"
     CDS             477..1178
                     /locus_tag="KRH54_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBG8581255.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            1228..2310
                     /locus_tag="KRH54_003655"
     CDS             1228..2310
                     /locus_tag="KRH54_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBG8581256.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFMNHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            2571..3671
                     /gene="vanG"
                     /locus_tag="KRH54_003656"
     CDS             2571..3671
                     /gene="vanG"
                     /locus_tag="KRH54_003656"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBG8581257.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            3668..4474
                     /locus_tag="KRH54_003657"
     CDS             3668..4474
                     /locus_tag="KRH54_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBG8581258.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            4492..6630
                     /gene="vanT"
                     /locus_tag="KRH54_003658"
     CDS             4492..6630
                     /gene="vanT"
                     /locus_tag="KRH54_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBG8581259.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKHLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVNMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINDCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLAE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            6944..7564
                     /locus_tag="KRH54_003659"
     CDS             6944..7564
                     /locus_tag="KRH54_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBG8581260.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(7730..8266)
                     /locus_tag="KRH54_003660"
     CDS             complement(7730..8266)
                     /locus_tag="KRH54_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBG8581261.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(8379..9053)
                     /locus_tag="KRH54_003661"
     CDS             complement(8379..9053)
                     /locus_tag="KRH54_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBG8581262.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLIATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            9256..11130
                     /gene="asnB"
                     /locus_tag="KRH54_003662"
     CDS             9256..11130
                     /gene="asnB"
                     /locus_tag="KRH54_003662"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBG8581263.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(11222..12658)
                     /locus_tag="KRH54_003663"
     CDS             complement(11222..12658)
                     /locus_tag="KRH54_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBG8581264.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESSLVKIFANNFIEIVN"
BASE COUNT         4808 a         1458 c         2047 g         4503 t
ORIGIN      
        1 aaaaggtgat tttgaggata ttatgaaggc tgatatacta taatttaaat acattaaaat
       61 aaaaaggagt tgtcttaaaa ttgatttttt tctaatcaaa ttttaaattc aactcctttt
      121 ttgaattttt atataaaata acatggaaat tttagtaatg tctgtggtta catacatagg
      181 tttaattgtt ttttatatgg attgtctctg gtataataaa ttgaattgtg tttgactgat
      241 agatattttt attgaaagtt aatacctgtt atgcgataat aaggacgtgt taggggagtt
      301 tttataataa caattaatct cagtttttgt gttccaatag tagcatcaac acattctgat
      361 gtagtagttc ttttcttata aaaattagtt acaaatatgc ttgtaattat ttgatatgta
      421 aatttaccaa tattataaat gatgacctcg ataataagga gagtgaaaaa taatctatga
      481 acgaaaatat tttgattgtg gatgatgaaa aagaaatagc agatttaatt gaagtatatt
      541 taaaaaatga aggttttaca gtatacaagt tttatacagg taaggaagct ctagaatgta
      601 ttgaatctaa aaatttggat atggctattt tagatattat gcttcctgat attgatggat
      661 ttcatatttg ccagaaaatt cgagaaaaat actattatcc tatcattatg ttaacagcta
      721 aaattgaaga tatagacaag ataatgggtt taaccatagg tgctgatgat tatataacaa
      781 agccatttaa ccctttggaa gttgttgcaa gagtaaagac acaattaaga agatatatgc
      841 gatataataa ttcttatgaa caacagtcga taattgttaa tgagtatgat attaaaggat
      901 tgattatcaa taaagaaaca cataaatgta gtttatttgg gaaagaagta gcattaacac
      961 cgattgaatt ttctattctt tggtatttat gtgaacatca aggaaaagtg gttccttctg
     1021 aagaattgtt tgaagctgta tggggagaaa aatatcttga taataataac acagttatgg
     1081 ctcatatagg gcgattacgt gaaaaaattc atgaaccttc taaaaacccg aaatttataa
     1141 aaactgtatg gggagtaggt tatacaattg aaaagtaaaa atattaaagt caatcctgac
     1201 tttactcaag tgagaaataa aatttttatg agaacagctt taatggcatt tatagccatt
     1261 attttcatat ggattttata ttcttttata ttgtatggta agttttctat ctggatagta
     1321 tcattctttg aaaatgcttt tcgtatggaa tataaagctg cattgaattt atatcagcag
     1381 attttccgta atagcatgga gctcttcatg atactagcta tggtgattgt gttttttata
     1441 atattccgtg tttatttaaa ttggtttaca aaatatttta gtgaaataaa tcaaggtatt
     1501 gactctctta tcaaagaaga tgttggagaa gtggctttat ctccagagtt attggcaata
     1561 gaaaagaaaa taaactcaat taaacatata cttgaacaac gtaaatttga gacacaaatg
     1621 gcagaacaac gtaagaatga acttattgtt tatcttgcac atgatttaaa aacaccactt
     1681 acatcagtta tagggtatct gacactactt cgagatgaaa gtcagatttc tgaagaactg
     1741 cgaaaaaaat atttatctat ttcgttggat aaggcagagc gtcttgaaga tttaattaat
     1801 gaattctttg aaattacaag gtttaattta tcaaatatat cacttgaata cagtacagta
     1861 agcttaactc gtatgcttga acaactaaca tatgagttta aacctacttt gatgagtaag
     1921 aatttaaaat atacactgaa tgttacacca aatatgatga taaggtgtga tgttaataaa
     1981 atgcaacgtg tatttgataa cttattacga aatgctgttt attatagttt tgaagatact
     2041 actattgaaa taacagctgt acaggaggat gattatataa agcttaaatt tatgaatcat
     2101 agcaatacga ttccagagga aaaacttgag cgtatttttg aacaatttta caggttggat
     2161 acatctcgta attcaaacag tggtagagct gggttgggac ttgctatagc taaagaaata
     2221 gtggagcttc ataacgggac aataacagct catagcgaga acaatataat tacctttaag
     2281 gttattattc cgattgtagg aaatttgtaa gaatttaagt ataaaaaaat aggaaattct
     2341 ttaggataaa taaataaaga gcactcttgt tattgatata attactgtat caaaacaaga
     2401 gtgcttttat tttgatacag taattatatc aatagaaaaa tgtgaaaata tattatgtgg
     2461 aacagatgca attgtaagtg aaggttgatt ggatttgaaa tagaaaaagt attttaacat
     2521 gtttctgtag attgtataaa aataaataaa tcaacaaagg agaatcaaag atgaataata
     2581 aaaaaattgc agttatattt ggtggaaact caacagaata tgatgtatca ttacaatcag
     2641 cttttgcagt acttgagaat ttgaatacgg aaaaatatga tattatacca attggaatta
     2701 caagggaggg taattggtat cattatattg gagattataa taatatccag aataacactt
     2761 ggtttgaaag ggatactgat ataattccag ttattgtttc gcagaaccgt gtcaaaaatg
     2821 gtattattga atttttaatt cctgatgctg tttctcagaa tcatattcca aatagtgcta
     2881 ttgaatcatg tgaaaatgat attaaataca ctaaacttga ttttgtgttt cctgtactgc
     2941 atggaaaaaa tggagaagat ggaacagtgc aggggttatt tgaattggca ggtatacagg
     3001 ttattggatg tggtactctt tcttcagcat tatgtatgga caaagacaaa tcacataaac
     3061 ttgttcattc agaaggtatt aaagtaccaa aatcaatatt actaaaatac tcagataaat
     3121 ttaatgtggc tgaactgtca aaagacttaa cttatccatt atttataaaa cctgtgcgtg
     3181 caggttcatc atttggtatt agtaaaattt ataaaaaaga agagttacaa gtagcagtag
     3241 acttggcttt tgaacatgat aatgaagtaa taattgagga aaatattgat ggttttgagg
     3301 tagggtgtgc agttttagga aacgatgact tacttgttgg aagagtagat gaaatagaat
     3361 tgtcaaaagg attttttgat tatacagaaa aatatacatt gaaaagttca aaaattcata
     3421 tgccagctag aattgatttg gattgtgaga aaagaataca agagacagca aaaattattt
     3481 atagaactct tggatgttct ggatttgcta gagtagatat gtttttaact ccatctggtg
     3541 aaattatttt taatgaagtc aatacaattc ctggctttac ttctcatagt cgttatccaa
     3601 atatgatgaa aggtattgga ctgtcttttg aagaaatatt ggacaagata attgaggtgt
     3661 acataaaatg atgaataata ttttattaga taaaaataaa gaagataaaa tgacaaatac
     3721 tatcttattg actaaagata aagtttatga tgggaatcta attctagtaa atgctttttt
     3781 accagttaaa acatcagaag atatagattt aattcctgta gatacaaggt ttccaagtat
     3841 tcttatgaaa cgtgaagcta caaacatttt acaaaatatt ttaaaaagta tatgtggaat
     3901 aaatgaaata gtacctgtaa gtggatatcg tactgcagag gaacaacaag atatatattc
     3961 aagttctctg cgtgacaatg gaaaagactt tactaaaaag tttgtagcac ttccaaatca
     4021 cagtgaacat cagacagggc ttgctattga tttagggtta aagaaagaag ttatagattt
     4081 tatctgccct gaatttcctt ataatggtat ttgtaatgag tttcgtaagg cagcactaca
     4141 ttatggtttt atagagcgtt atcagtgtaa aaaagaagaa ataacaggaa tatctcaaga
     4201 accatggcat tttcgttatg tgggatatcc gcattctgag attatcaata aaatgaatct
     4261 cgctcttgaa gaatatatag aatatattaa gagattttcg tatacaaatt gtttaaaaat
     4321 agaagataaa gaaaaaatta tagaaatctt ttatgtgcct atccatacta aagaagaagt
     4381 gagaatctct atacctgaac aatgtgtata tcaaatatcg ggtaacaata aggatggttt
     4441 tattgtgaca ttttggagaa atagaaagga ctaagaaatg gaggaacaag aatgattagg
     4501 aataaatctt atgcagggct tgattatttt aggattatag ctgcattact tataattgca
     4561 attcatacat ctccattatt atcatatagt aaaatggctg actttgtgtt aactagtata
     4621 attgctcgta ttgctgtgcc attctttttt atgctatcag gcttttttct aatttcaaca
     4681 tataattata attgtaataa actgaaatct tttctaaaaa atgtattatt tatttacggg
     4741 atagctattg taatttatat tcccttaaat atttacactg gatatttttc catgaataat
     4801 ttagtgccca atattattaa ggacattgta tttgatggga ctttatatca tttatggtat
     4861 ctacccgcat caattattgg tgcaagtatt gcatactttc ttgtaaaaaa actaggttta
     4921 aagtactcac tagtaatcac tattgttatt tatgtaattg gtatgtttgg tgatagttat
     4981 tatggttttt tagaaaaaat accactatta aagaatttat atgggtatat ttttgaagta
     5041 tcagattata cacgaaatgg tatatttttt gcacctgtat ttttcatact tggtggaata
     5101 atatctaata aatctaatta tatgtcaact aaaaatagct tgattggatt tcttatatca
     5161 ttcttactta tgttatgtga gggaatgttt ttacataaac taggggtaca gcgtcatgat
     5221 agcatgtata ttatgttatt gccatgtatg tatttcttat ttacagcctt aacatttttg
     5281 aaaggttctc gagctaggtt attgcgaaca tcatcattaa ttatttatat tatacatcct
     5341 atgatgattg ttgttattcg tatgttttct aaaatattcg gaatacaaac tgtcttggtt
     5401 gacaatagcc ttattcatta tatagtggta agtatagcat cttttatagc ttctataatt
     5461 attatattga ttcttaaaca tctaaaaaaa gacttgaaag gagaacataa ggtaaatacc
     5521 aatagagctt ggattgaaat tgatttaaat aatttggaac ataatgtaaa tatgatacaa
     5581 aaagctatgc cagaagattg tgaattgatg gcagttgtaa aagctaatgc ctatggtcat
     5641 agtgcttttg aagtagcgac ttgtgcaaac caaataggtg taaaagcatt tgcagtagca
     5701 acaatagatg aggggattga tttacgtcga tatggaatac taggagaaat attgattctt
     5761 ggatatacta acccaataag ggcaaaggaa cttcataaat acaatcttac acagagtgtt
     5821 attgacctta atcatgctat ctctttaaac aaacagaaat acaacataaa atcacacatt
     5881 aaaattgata caggtatgca cagactgggt tttgatgtta atgatgtgag gtcaatttta
     5941 aaggcattta aattaaaata cttagatata tgtggcatat ttacacactt gtgtgtttca
     6001 gatagtctga aagatgaaga tgtagacttt acaaataaac aaattgaatg tttttataag
     6061 ttaattgatt ttctgttaaa aaaaggtata aaaattccta aagtacatat tcaaagtagt
     6121 tatggttttt ttaattatcc caatcttaag tgtaattatg taagaattgg aattgcatta
     6181 tacgggatat taagttctcc aaatgatacg actaaattgc aacttgattt acgacctgtt
     6241 ttatccttaa aatcacaagt aattcttatt agaaaaatac aaaaagatga tagttttgga
     6301 tatggaagag tctttattgc aagcagagat agcgtaattg ctatattatc tattggctat
     6361 gccgatggta tacctagaaa tttatcctgt ggaaaaagtt acgtaataat aaatgattgt
     6421 agagcagcaa tagtgggtcg aatttgcatg gaccaacttg ctgttgatat tacagatata
     6481 cctaatgtag aagttggtaa tacggcaata ataataggaa gagataattt agctgaatta
     6541 tctgcttctg aagttgcaaa taactcatgt agtatatcaa atgaattatt gagcagagta
     6601 ggaagaaggt taaatgttat aaagaagtaa ctcaattaaa tagtaatatt tgttatactg
     6661 tgtaatttat tttactctga ctagacattt ttagtaatat tatgtctaat atatattttt
     6721 tataacttac atgacaattt aagttataaa aaggtgatgt ttcagatttt tattttgaaa
     6781 catcaccttt tttgttataa tatataataa aataattata ccttaactgt aaacttatat
     6841 agattattta ctttttaata ttagattcat tttttgagat tggtatataa taaacatata
     6901 gagtatataa taatagttaa agttaaaaaa ggggaaaagc aatatgcagt atatacaaga
     6961 actacttgat ttgactcaaa attattgggt gttagccaca ataatcggct tattaagtgc
     7021 atttatagaa agctttatac cagccttacc acttgttgct atagttacag caaatgctgc
     7081 aatacaagga ttatttattg gatgtttgtt atcatggatt ggctcaggac ttgggacaac
     7141 ttcattattc ttattaataa gcagatttaa tgatagtaag ctttttaata agttgagaaa
     7201 tagtaaaact gaaaaagcaa taagttggat ggataaacag ggatttaagt tattatttat
     7261 agcgtatgcc tgtccattta tgcctggttg tttagttact atcgcatcag cttttggtaa
     7321 aaaagattta aaagattttg tgccagcaat gttggcagga aaatttgtaa tgtttatagt
     7381 tataagttac gtggcaagtg atatagaagg ttttataaca agtcctttaa agatagcatc
     7441 atttatatta ttggtgtttt tgtcatggaa gattggaaat agagtaaata aaaatcttga
     7501 aaatcataat tatgatttcc atcataaaaa acatcacaat gataaagatg ataaaatgat
     7561 ttagcttgta cgaaataaaa cgtatcttaa aatgatttag tactaaatta ttttaagata
     7621 cgttttattt tttgtattaa ggtacaaaat ttttagttct tttgtaaact aaaatatata
     7681 aaacttattt atacttagtt caaaatattg ttatttattt tttctacaac tataataaaa
     7741 tataaaattg atatccaatc aaaaaaataa ctatgaaatt taatagctta gatatgaatt
     7801 tttctgtttt tgagttagtt tttataggta gtaaagaaat gtttatggta atgggataaa
     7861 acaattcaat accttcttta gtaataaggt caagacacaa gtgagataga tatcctatta
     7921 taaacccact ggacaaacat gagaaaacta tattattgtt agtgaagatt gtcagagatt
     7981 taaaaaaact ataaataagt aataaggcaa ttagactgtg agtaaaccct ctatgtctaa
     8041 acctacttcc aaataatttg tatattaaca taaatttttt acttatgtaa gaacctctca
     8101 tatctatatc tggaaataaa gaacctaaat atgcaaaata aatataaatt ataattaata
     8161 ctattttata tgcaatatcg tatttaacta ggtacagatt gtttataaag gggagtatca
     8221 aaagtgcgaa tatataacct cccctttcat gtgtttcttt tatcatataa aaatcctcct
     8281 gaatcgtcac tataaacatt atattggtaa atttagacta tcttttatta tattatatag
     8341 caaacataag tttttttcaa aaggatttta atatttaatt aatcttgaga ttttaaattt
     8401 ttgtaatttt ctaaagcacc attccaattt actacattaa accagttgtc aatgtattca
     8461 tttcttctat tttgatattt taaatagtaa gcatgctccc aaacatcaag tcctattata
     8521 ggagttaggt ttttacttac agggctatcc tgatttggag tagtcataat agataatttg
     8581 ccatctttag tagctataag ccaagcccaa ccagaaccaa agacatctaa agcagatttt
     8641 tggaactctt gcttaaattt ttcaaaagaa ccaaagtctc tatcaatagc ttcttttaaa
     8701 gattcagaag gtatggtttt ttctggtgtc attatatcaa aaaagaattt atgattataa
     8761 gctccacctg cattatttct tacagttgta gcaatatctt taggtaaaga atctaaattt
     8821 tgcaataatt cacataaaga ataattataa agctcaggat atttttcaag agcagcattt
     8881 aatttatcaa cataagcttg ataatgctta tcatgatgca gtttcattgt ttctttatct
     8941 atataaggtt caagtgcatc atatgcataa ggtaatggtt taaccttaaa tttgttattt
     9001 tcaggtgtaa aagcaaatga agttatgcac tgtgagatta taaataaaga cataataact
     9061 ggtattaata tttttttctt cataaaaatc acctcctaat atagtatttc tagaaataaa
     9121 aaaaatatga aatattaaca gataatttat aagaatttaa tagcaatttc attttaaatt
     9181 gaataagttt taaagccaaa gacaaaacta tgaaatatag aataagacta aaggacagaa
     9241 aggaattttg attatatgga cggcaatatt tcaatttatt ataaaggtga tatatctaaa
     9301 aaagaagata ttaaatttaa aataaaagaa tctgtaaata atacaagact ttctagtaga
     9361 atagagtata aggatgagga aaaaactttt gattttgaaa gatttaatgt aattgatgct
     9421 atttatggaa ataaaccatt tgaaaaagat ggaaaagtta ttgtttttaa tggaaatata
     9481 tataactatc atgaaataaa agaagaatta attaaaaaag gacacaattt tactacaaat
     9541 gatgatattg aagtcttatt agcatcttat atagaatttg gtaagaattg tgtacataag
     9601 attaaaggaa tgttcaactt tataatctat gatagagaaa atgaaagtat atttggagca
     9661 agagatttat ttgggataaa accactttat tatataaata aggaaaatgc aataattttt
     9721 tcctcagagt ataagttttt acttgaatat atgaaaaatt taaatataaa tgaaagaagc
     9781 ttacaaagtt atttttcatt tcaatatgta cttcctgaag atactatgat acaaggaata
     9841 aggctgatac cagcaggtca ctattttagg gtagaaaatg gtatactatc tttaaagaga
     9901 tataataaac ttgagtttag aagtagtaca aaattttttt atactaaaaa tcacttaggc
     9961 aatagagatg tgaatgagga tgatataaga aatatagtag tagattcaat agtaactcat
    10021 atggaagagg agaaagaaat tggaacattt ttgtctggtg gaatagattc atctattata
    10081 actactgtgg cttcacaaat taatccaaat ataaaatctt tttctgcagg ctttagtgtc
    10141 aaaggatata gtgaattaga agtagcaaag aaaacagctg ataagttagg tatagaaaat
    10201 atccagatta atataacaca agatgaatat ataaaatcat taccgaatgt aatttattct
    10261 ttggatgacc ctatagcaga cccttcagaa gtaggtcttt atttcttaat taaagaggca
    10321 ggtaaacatg ttaaagtagc attgtctgga gaaggagcag atgaattatt tggaggatat
    10381 aatatatata aagagtatag tactatgaaa tcagttgtca attcacctac ttatattaaa
    10441 agtatattag gaagagtttc agaacttatg ccaaacataa aaggaagaaa ttatctttat
    10501 agagctacaa caccattaga aaaaagatat ataggaaatg caaaagtttt tgaaaacagc
    10561 gaagtaaaaa gattcttttt taagtataag gaaaagaata tatatgaata cctgttgtct
    10621 aatttgtata gagatgcaca aaaaaataat tacgattata taagtaaaat gcaacatata
    10681 gatgtgaata catggcttca aggcgatata cttcaaaaaa ttagcaaact ttctacagct
    10741 gagcaggttg aacttagagt accattttta tataaagatg tatttgatgt cgcaaaaaat
    10801 ttaagaatgg agcaaaaaat aaataagaat aatacaaaag ttttacttag agaggcgttt
    10861 agagaaatag tcccagaaca tgttgttcaa aggaaaaaat taggatttcc aactccaata
    10921 agagtttggt taaaagattc tctaggggat attgtaaagg aaactattta taattcaaat
    10981 gtagatgaat ttatagataa aaaatatgca ataaaacttt tagatacgca tcttaaaggt
    11041 catagagata attcgagaaa aatatggact atatttacat tttgtctatg gcatcaatta
    11101 tttattgagc ataaaaatgt cgagtattaa tacttttttc aatattaatc agagggcaaa
    11161 agcatgcaat gttttcaccc tcttgattat tatggaatag gataagtaga attttgattg
    11221 attagtttac tatctcaata aaattattgg caaatatttt gacaagacta ctttcaacag
    11281 ctaaatttaa gttttgtcca tcatcatatc cagcggaaat caatcctagt aagtttccaa
    11341 acatatctaa aagagctcct cctgaactac cactagagat tggagctgta aattgtatca
    11401 tttgaaccgt ttcaaaatca cgaaaagctg aaataattcc atctgaaact gtattaaaaa
    11461 gacctagagg gcttccaatt gcaactattt tttgacctct cacaggtttt ttactgactt
    11521 taacaggtat tggcttgcat tttctatcaa cctttatcac agctaaatca taatctgaat
    11581 ggtattttat aatttggtaa gatgtataaa ctttatcatc attttcaaat ctaactaaaa
    11641 atgaataccc atcattgact acatgaagat tcgtaagtat ataaccttca ttatttatta
    11701 ctactccaga accgctctta aacggtttat tttcgttatt gcacacaaca atcataacaa
    11761 cagaagctga taactttgca aggtcttcat aagaaagttc tacttcctca tcttgtacaa
    11821 tttttttaga tgttagattt gtgttattaa gtgtattttt agagttaggt ttattcgtag
    11881 taggagaagt cttattggta ttttcactgt aatttatatt tgtaatatgc ctttcaaaag
    11941 gtatttctaa tatatttgga gtcttcctag aaagttcaaa tagagtagaa aattttgaac
    12001 attcaggata taaatcaaga atatctttat ttggatttat atataaaata tcacacccaa
    12061 gttgtgataa aaaatataag aaaaaaagtt cttgcctttt aatatttcca aagtatacta
    12121 tctttggtga aatagtagtc ttcaccttgt tataaaataa tttaggtaga aaatagtcta
    12181 tccaacacaa aagtttaact gcaaagtttt tgatgataga ggatgtacaa tttggcatac
    12241 ttgtggaaaa taaatctaaa gtatttttaa atgcaatctt tttagtccaa tttaaggtgt
    12301 cattttctat tttacatgga aagtcgatat tatataaatc ccaactgtta aacttagaac
    12361 tattataaat attaaaaatt ttaagcatct tatctgtttg ttctttgtca aaatttattt
    12421 ttagattgtt taatctaata tagggaattg taggattatt aaaaaaataa gtatctatat
    12481 ttttaatttt ttctatgtat ttatcaattg aatcatcaac acctatatat cttgtaaagt
    12541 agacttcttt aatatatgaa gaactcttat ttttataggt agtatcagat ataaattcaa
    12601 ttagaggttc agtagacttt tttattgaaa tctttaatgg tttaatagta caaatcatat
    12661 tttttctcct ataataagat gatataatct attataaaac aacttaaaac aatatagtac
    12721 aataattttg tagtttcaaa ataaaatatg tacttgttta tataaatgat atataattac
    12781 aataaaattg ccgaaaagcc ctctccttta ggtgta
//
LOCUS       DAEOSP010000426        11489 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.guided.127, whole genome shotgun sequence.
ACCESSION   DAEOSP010000426 DAEOSP010000000
VERSION     DAEOSP010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11489
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.guided.127"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(<1..746)
                     /locus_tag="KRH54_003664"
     CDS             complement(<1..746)
                     /locus_tag="KRH54_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBG8581265.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIV"
     gene            1480..2638
                     /locus_tag="KRH54_003665"
                     /pseudo
     CDS             1480..2638
                     /locus_tag="KRH54_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608406.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2790..2873
                     /locus_tag="KRH54_003666"
     CDS             2790..2873
                     /locus_tag="KRH54_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBG8581266.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            2998..3735
                     /gene="erm(B)"
                     /locus_tag="KRH54_003667"
     CDS             2998..3735
                     /gene="erm(B)"
                     /locus_tag="KRH54_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBG8581267.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            3680..3853
                     /locus_tag="KRH54_003668"
     CDS             3680..3853
                     /locus_tag="KRH54_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HBG8581268.1"
                     /translation="MSKYCLFLIVIYYLTGGNNSMSRFCKFGKLHVTKGNVDKLLGIL
                     LTASKELKRSGPH"
     gene            4097..4336
                     /locus_tag="KRH54_003669"
     CDS             4097..4336
                     /locus_tag="KRH54_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8581269.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSLIESGKSDPTL
                     KTLERIAELTNSTLVIDLIPNELEQVELQIEEEKQ"
     gene            4731..6221
                     /locus_tag="KRH54_003670"
     CDS             4731..6221
                     /locus_tag="KRH54_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBG8581270.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KKSMIGGRGVVLTSEEAIHENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTLIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKLVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREALGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALSEFFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            6224..6616
                     /locus_tag="KRH54_003671"
     CDS             6224..6616
                     /locus_tag="KRH54_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBG8581271.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            6617..8056
                     /locus_tag="KRH54_003672"
                     /note="aph(2'')-Ia"
     CDS             6617..8056
                     /locus_tag="KRH54_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HBG8581272.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(8186..9358)
                     /locus_tag="KRH54_003673"
     CDS             complement(8186..9358)
                     /locus_tag="KRH54_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HBG8581273.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFEPYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            complement(9393..11468)
                     /locus_tag="KRH54_003674"
                     /pseudo
     CDS             complement(9393..11468)
                     /locus_tag="KRH54_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610982.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
BASE COUNT         3902 a         1813 c         1959 g         3815 t
ORIGIN      
        1 actattaact ctggtctaaa ttctaatcca aaagctgctg gtgttggtaa agatactata
       61 gctgctcctt gaacggctga gaaatcaacc aatcctaaaa ctaaagatat agcataacct
      121 actaatattc ctattaatat tgaagaattt tttactaaac ctttaccgta tttatttatc
      181 attattatta caaaagcaac tataactcct actgtgaagt ttattgggct tccaaatgtt
      241 tgtgtgcctt ctcctccagc taaattcttt atagctgttg cgaatagtcc aagtcccata
      301 catgctatta tagttccaga aactattgga gtgaatatat gtctaatttt cttaactcca
      361 aatcctacaa agaatacgat taccccagca gcaatctgtg ctccaaatag agctgctaaa
      421 ccatctttag ctgctactga aagacaagct cctaaaaata cataactcat tcccatcatt
      481 acaggtaatc ttgaacctaa cttaaatcct ttaaataaag gtattggata taactgtaaa
      541 aacgttgcta aacctgctgc taacatagaa ccctgaatca acattgttgc ttgcccctgg
      601 ttaagtccta ccacgtttgc tattaatatc gcaggtacca tatttcctac tatcattgct
      661 aacagatgtt gtaaggccaa aggtattgat tcctttagtg gtggtatccc atcgacatca
      721 tattttgaag tattacgtgt tttcataaaa taaatcctcc catacataaa tatcttgact
      781 aaatattttt ataattattt tctataaata tttttatgac catgaattta ttgctcaacc
      841 gataaacaca gctaaactga gctattttta taaaaaatta acaaatttct aaatattgcc
      901 tccccgattt tcaacttaaa tgaacattaa ttgcctggta tatatttatt tgttaaattt
      961 tttattaaaa tgaatagcta tcaaaaataa tctaacaatc tcatactaaa taatttaaac
     1021 aattatattt aaattttata tctgattttt gattatccgc ataaacacta tactttcaag
     1081 cattttgaaa tataggaaag cactcatttt accacgaatt taccacgatt gaatgagcct
     1141 tgatatgacg taagaatttg aaaaggacac cgtatagttt ggtgtccttt tacatgaaaa
     1201 tcattagtcg ctattgattg ttattataca ttttgttaat tgttcttatt tcttttacgt
     1261 tttttaccga aaatcatgag tattgtccga gagtgattgg tcttgcgtat ggttaaccct
     1321 aaagttatgg aaataagact tagaagcaaa cttaagagtg tgttgatagt gcattatctt
     1381 aaaattttgt ataatggtat cgtaatttaa gacacttcaa gagacatttc ttaatgaatc
     1441 tgatatactg taaacactac agaaagaagg attattatta tgtctcgaac aagaagaaat
     1501 ttctctgcca aattcaaatc agaattagtg attgaactgc tcaaaggaga aaaagactta
     1561 aatacaatcg caaccgaaaa caatattcag ccgaatcttc tccgcaactg gaagaaggag
     1621 ttcctcgata aagcatccgt ggtttttaat gacacacgag aggataatct gaaagaaaaa
     1681 ctcgctttag aacgcaagga aaaagctgaa tatgcgaaaa aagttggcca gctcaccatg
     1741 caggtggatt ggttgaaaaa aaaatctgaa gaaacacttg gacctgacta cgagagtaaa
     1801 tttagtccaa aaccttttga agactaaaga acttccagtt aaaacaggag ctacacttct
     1861 tgatatcaat cgtaccagtg tttattacaa gggcatgccc atatctcagg aggagttgga
     1921 ttgcaaatcg atcatagatc gattacacac ggataatccg gcctggggag cacgacaact
     1981 gtctgctcaa ctgaagaagc gtgggcatca ggttggtcgc cggaaaacgc gccgttatat
     2041 gaatgaaatg gggattgatc caatttatcc aaaaatgaac ctttctaaac gtatgcgaca
     2101 ggctaaagtc tgcccgtatc tgctacgtaa cgccgttatc gaccgtccaa atcaggcatg
     2161 gtcaatcgac attacataca tccccattaa gcgtggattt ctgtatctga ccgctgtgat
     2221 tgactggtac agccgctgta tcgttggctg ggaagtcgat gatactctgg ataccagaat
     2281 ggtcataact gcgttaaaaa aggcgtttat agtggcaaaa cctgttatcc tgaattcaga
     2341 tcagggctgt cagtttacaa gcaatgagta catgaatttc ctcaaagaga accagatccg
     2401 tcaaagcatg gatggtaaaa gccggtgggc tgacaatatc atgattgaac gatggttccg
     2461 aagcttcaag tacgaggaag catatctgac ccaatataac aatatcagag aagcacggaa
     2521 ggcaatcggt aaatatgtgc acacttacaa ctttgaacgt tgtcattctg cactcaataa
     2581 gcaaacaccg gcatcctgct attatccgat tctgttactg gatgatcatg cagcctaagg
     2641 ggggattttc tcccctaccc agttacatat atcagttcat tataaaaagc ttagattttt
     2701 gtcttgacaa ctgaaccact atattgtata ataggaattg aagttaaatt agatgctaaa
     2761 aatttgtaat taagaaggag ggattcgtca tgttggtatt ccaaatgcgt aatgtagata
     2821 aaacatctac tgttttgaaa cagactaaaa acagtgatta cgcagataaa taaatacgtt
     2881 agattaattc ctaccagtga ctaatcttat gactttttaa acagataact aaaattacaa
     2941 acaaatcgtt taacttctgt atttatttat agatgtaatc acttcaggag tgattacatg
     3001 aacaaaaata taaaatattc tcaaaacttt ttaacgagtg aaaaagtact caaccaaata
     3061 ataaaacaat tgaatttaaa agaaaccgat accgtttacg aaattggaac aggtaaaggg
     3121 catttaacga cgaaactggc taaaataagt aaacaggtaa cgtctattga attagacagt
     3181 catctattca acttatcgtc agaaaaatta aaactgaaca ttcgtgtcac tttaattcac
     3241 caagatattc tacagtttca attccctaac aaacagaggt ataaaattgt tgggaatatt
     3301 ccttaccatt taagcacaca aattattaaa aaagtggttt ttgaaagcca tgcgtctgac
     3361 atctatctga ttgttgaaga aggattctac aagcgtacct tggatattca ccgaacacta
     3421 gggttgctct tgcacactca agtctcgatt cagcaattgc ttaagctgcc agcggaatgc
     3481 tttcatccta aaccaaaagt aaacagtgtc ttaataaaac ttacccgcca taccacagat
     3541 gttccagata aatattggaa gctatatacg tactttgttt caaaatgggt caatcgagaa
     3601 tatcgtcaac tgtttactaa aaatcagttt catcaagcaa tgaaacacgc caaagtaaac
     3661 aatttaagta ccgttactta tgagcaagta ttgtctattt ttaatagtta tctattattt
     3721 aacgggagga aataattcta tgagtcgctt ttgtaaattt ggaaagttac acgttactaa
     3781 agggaatgta gataaattat taggtatact actgacagct tccaaggagc taaagcgctc
     3841 gggaccccat taagtatttt taaaagcatg gtcgcaagtt tcactagcag ccatgctttt
     3901 attgaatcat tgtaggacat tttaaagata attgcccact ttaaaattaa acatgtgctt
     3961 agaatcgttc taggcaacac caataacaat cactaatcac aagtgatttg tgattaatca
     4021 cttgtgattt acaaggtgta acgatataat taaatggaaa acgtgcaaaa tatttacata
     4081 aacaggaggg agaaacttgg aactagcatt tagagaaagc ttaaaaaaga tgagaggtac
     4141 caaatcaaaa gaaaaattct cccaagaatt agaaatgagt agatcaaatt attcattgat
     4201 tgaatcagga aaatcagatc cgacattaaa aacattggaa cgaattgcag aattgacaaa
     4261 ctcaacctta gttattgact taattcctaa tgaattagaa caagtagagt tgcaaataga
     4321 agaagagaag caatgattgt cgttaatata tttaacgaaa ttgttgctta ttttttttaa
     4381 aagcggtata ctagatataa cgaaacaacg aactgaataa agaatacaaa aaaagagcca
     4441 cgaccaactt attttccgcc aagaaaataa gttgcgagcc ttaattgatt accaccaatc
     4501 aattaaagaa gtcgagaccc gaaatttggt aaagtattta attactttat taatcagata
     4561 cttaaatatc tgtaaaccca ttatatcggg tttttgaggg aatttcaagt ctttaagaag
     4621 ataccaggca atcaattaag aaaaacttag ttgattgcct tttttgttgt gattcaactt
     4681 tgatcgtagc ttctaactaa ttaattttcg taagaaagga gaacagctga atgaatatcc
     4741 cttttgttgt agaaactgtg cttcatgacg gcttgttaaa gtacaaattt aaaaatagta
     4801 aaattcgctc aatcactacc aagccaggta aaagcaaagg ggctattttt gcgtatcgct
     4861 caaaaaaaag catgattggc ggacgtggcg ttgttctgac ttccgaagaa gcgattcacg
     4921 aaaatcaaga tacatttacg cattggacac caaacgttta tcgttatggt acgtatgcag
     4981 acgaaaaccg ttcatacacg aaaggacatt ctgaaaacaa tttaagacaa atcaatacct
     5041 tctttattga ttttgatatt cacacggcaa aagaaactat ttcagcaagc gatattttaa
     5101 caacagctat tgatttaggt tttatgccta cgttaattat caaatctgat aaaggttatc
     5161 aagcatattt tgttttagaa acgccagttt atgtgacttc aaaatcagaa tttaaatctg
     5221 tcaaagcagc caaaataatc tcgcaaaata tccgagaata ttttggaaag tctttgccag
     5281 ttgatctaac gtgcaatcat tttgggattg ctcgtatacc aagaacggac aatgtagaat
     5341 tttttgatcc caattaccgt tattctttca aagaatggca agattggtct ttcaaacaaa
     5401 cagataataa gggctttact cgttcaagtc taacggtttt aagcggtaca gaaggcaaaa
     5461 aacaagtaga tgaaccctgg tttaatctct tattgcacga aacgaaattt tcaggagaaa
     5521 agggtttaat agggcgtaat aacgtcatgt ttaccctctc tttagcctac tttagttcag
     5581 gctattcaat cgaaacgtgc gaatataata tgtttgagtt taataatcga ttagatcaac
     5641 ccttagaaga aaaagaagtg attaaacttg ttagaagtgc ctattcagaa aactatcaag
     5701 gggctaatag ggaatacatt accattcttt gcaaagcttg ggtatcaagt gatttaacca
     5761 gtaaagattt atttgtccgt caagggtggt ttaaattcaa gaaaaaaaga agtgaacgtc
     5821 aacgtgttca tttgtcagaa tggaaagaag atttaatggc ttatattagc gaaaaaagtg
     5881 atgtatacaa gccttatttg gtgacgacta aaaaagagat tagagaagcg ctaggcattc
     5941 ctgagcggac attagataaa ttgctgaagg tactgaaggc gaatcaggaa attttcttta
     6001 agattaaacc aggaagaaat ggtggcattc aacttgctag tgttaaatca ttgttgctat
     6061 cgatcattaa agtaaaaaaa gaagaaaaag aaagctatat aaaggcgctg tcagagtttt
     6121 ttgacttaga acatacattc attcaagaga ctttaaacaa gctagcagaa cgcccaaaaa
     6181 cggacacaca actcgatttg tttagctatg atacaggctg aaaatggaag aagttttaat
     6241 tgatgataat atggtttttg atattgataa tttaaaagga tttcttaatg ataccagttc
     6301 atttgggttt atagctaaag aaaataataa aattatagga tttgcatatt gctatacact
     6361 tttaagacct gatggaaaaa caatgtttta tttacactca ataggaatgt tacctaacta
     6421 tcaagacaaa ggttatggtt caaaattatt atcttttatt aaggaatatt ctaaagagat
     6481 tggttgttct gaaatgtttt taataactga taaaggtaat cctagagctt gccatgtata
     6541 tgaaaaatta ggtggtaaaa atgattataa agatgaaata gtatatgtat atgattatga
     6601 aaaaggtgat aaataaatga atatagttga aaatgaaata tgtataagaa ctttaataga
     6661 tgatgatttt cctttgatgt taaaatggtt aactgatgaa agagtattag aattttatgg
     6721 tggtagagat aaaaaatata cattagaatc attaaaaaaa cattatacag agccttggga
     6781 agatgaagtt tttagagtaa ttattgaata taacaatgtt cctattggat atggacaaat
     6841 atataaaatg tatgatgagt tatatactga ttatcattat ccaaaaactg atgagatagt
     6901 ctatggtatg gatcaattta taggagagcc aaattattgg agtaaaggaa ttggtacaag
     6961 atatattaaa ttgatttttg aatttttgaa aaaagaaaga aatgctaatg cagttatttt
     7021 agaccctcat aaaaataatc caagagcaat aagggcatac caaaaatctg gttttagaat
     7081 tattgaagat ttgccagaac atgaattaca cgagggcaaa aaagaagatt gttatttaat
     7141 ggaatataga tatgatgata atgccacaaa tgttaaggca atgaaatatt taattgagca
     7201 ttactttgat aatttcaaag tagatagtat tgaaataatc ggtagtggtt atgatagtgt
     7261 ggcatattta gttaataatg aatacatttt taaaacaaaa tttagtacta ataagaaaaa
     7321 aggttatgca aaagaaaaag caatatataa ttttttaaat acaaatttag aaactaatgt
     7381 aaaaattcct aatattgaat attcgtatat tagtgatgaa ttatctatac taggttataa
     7441 agaaattaaa ggaacttttt taacaccaga aatttattct actatgtcag aagaagaaca
     7501 aaatttgtta aaacgagata ttgccagttt tttaagacaa atgcacggtt tagattatac
     7561 agatattagt gaatgtacta ttgataataa acaaaatgta ttagaagagt atatattgtt
     7621 gcgtgaaact atttataatg atttaactga tatagaaaaa gattatatag aaagttttat
     7681 ggaaagacta aatgcaacaa cagtttttga gggtaaaaag tgtttatgcc ataatgattt
     7741 tagttgtaat catctattgt tagatggcaa taatagatta actggaataa ttgattttgg
     7801 agattctgga attatagatg aatattgtga ttttatatac ttacttgaag atagtgaaga
     7861 agaaatagga acaaattttg gagaagatat attaagaatg tatggaaata tagatattga
     7921 gaaagcaaaa gaatatcaag atatagttga agaatattat cctattgaaa ctattgttta
     7981 tggaattaaa aatattaaac aggaatttat cgaaaatggt agaaaagaaa tttataaaag
     8041 gacttataaa gattgattat ataatatatg aaaagctatt ataaaagaca ttagtattaa
     8101 atagtttaaa aaaatgaaaa ataataaagg aagtgagtca agtccagact cctgtgtaaa
     8161 atgctataca atgtttttac catttctact tatcaaaatt gatgtatttt cttgaagaat
     8221 aaatccattc atcatgtagg tccataagaa cggctccaat taagcgattg gctgatgttt
     8281 gattggggaa gatgcgaata atcttttctc ttctgcgtac ttcttgattc agtcgttcaa
     8341 ttagattggt actctttagt cgattgtggg aatttccttg tacggtatat tgaaaggcgt
     8401 cttcgaatcc atcatccaat gatgcgcaag cttttgaata ttttggttga tcgatataat
     8461 catgaatcaa tcgattttta gcctcacgcg ctaagttaat atctgtgaac ttaaaaattc
     8521 ctttaacagc ttctctgaaa gattttgaat tttttttagg aatggtggta aagatatttc
     8581 ttaggaagtg aacttggcat ctttgccaac ttacgttggt gaaggatttt ctaatggcag
     8641 agactaatcc tttgtgcgca tcagaaataa cgagttccgt accttgtaaa ccgcgttctt
     8701 ttaggtattc aaaaaatgtt gtccaggtct cttcgctttc gccactttga atcatgaagc
     8761 cgataatttc acggtcgcca tctttggtta ttccaatcgc tatatgacag ctttttgaga
     8821 gtactcgatt ttcttctcgt acttttatat agagtacatc ggtcattaag taaggataat
     8881 ttttttctga taataaacga ttctgccact cgttaaccat aggttctagc tgttctgtta
     8941 agctagaaac gaaggactta gagacggatt taccacaaag ttcttccaca atttttgata
     9001 ctttacgagt tgaaacgcct gatacataca tttccaacat tgaagccatg agggcttttt
     9061 cgtttcgttg ataaggttca aacactgtgg gtgaaaaatg gccatcacgt gttctgggta
     9121 cttttaattc tagcgtgcct acacgtgtcg taaagctgcg ctcataatag ccatttcgtt
     9181 gactttgtcg gttttctgtt cgttcatatt cttttgcttg aatatattct gttcgttgat
     9241 tttccattag ttgattaaat accgttgtta aaatattttt agaaacgtca tcctttacag
     9301 aatattcaat aatgctttga atctcttcgc ttttcagtgt aaaatgtact tgggtcatgt
     9361 aaaagtcctc ctgggtatgt ttttgtcgtt aataaaacag ctaatacacc ggttgaacca
     9421 gcaaacatag aagtaaacaa tcctacgttt gtcatatcac ctgtttttac agggttcttt
     9481 tgtccgctag gctttgttgg agtagttggc ttgctcggtt tctgtggctg ttgtggactt
     9541 acaggtttat ttgttacttt ttcttttacg gtaactgtaa ttgttttttc agtaattgca
     9601 ccatttttat ctactacacg gaaagttaca tgataagtgc cagccttaga agtatctaca
     9661 tcatttgcaa taatgttatc ttttgttaaa ataatcgttc cgtcctcttt atctgttgct
     9721 gtcacatttg ccaatgggtc gaacgtatcg cctacattca atgtaacatc atttgctgaa
     9781 atgattggag cagagttaag gtcgcctgta ttttgtttca cagttacagt aatcgttttt
     9841 ttagcagaag ctccgttttt atctgttact ttataagtca catggtaagt accagcctta
     9901 gaagtatcta catcatttgc aacaatatta tcttttgtca aagtgatagt tccgtcctct
     9961 ttatctgttg ctgttacatt tgccaatggg tcgaaagtat cgcctacatt caatgtaaca
    10021 tcatttgcag aaatagttgg tgctgtatta tcttctcctt gtgagtttaa cgataattct
    10081 acttttaaaa caacgtttcc gtctttataa gtaccgcaat catactcata agtaactggt
    10141 ttttttacat catctaccgt tataattcct gtatctttat ctaaagttcc atttgcagta
    10201 acttttactt ttgaaatatc tattccttgt tcaacatctt tttttaagtc gattttattc
    10261 cctttaatat caatataacc gttgcctaca aaagcatgat tattttcaag aactttattc
    10321 tctccgacat ttaaccagta taaatcatcg caaattaatt ttttaatttt tgtgttttta
    10381 cttatatcaa gacctcttat cccttgttca aaaatttcac cgcaacgtag catttcaagt
    10441 tctaaattat tgcttaaatc taatttttta atagttgtat catgacaaaa aaggcgtgtc
    10501 agttttgtgt tttttgttac atctaattca gctatatttg tcatataaca tgatagttgt
    10561 ttcaaattag ggttcttact tatatcaagt gtttttactt ttgtatctcc tatatctaaa
    10621 gtgactaatt gcggattatg tgtgacatca agttccatta atcctgtatt gctacaagat
    10681 agttgttcta agttaggatt gtttgttaca tctaaattgg aaatagaggt actatctgca
    10741 aataacgtat tcaatttttc attcttagat acatcaagac tgttaattga tgtattatcg
    10801 caaacgaaag cttccaaatt cggataacgt gttaaatcaa cgcttcttac ttttgaagaa
    10861 cgacaatcta atctttttaa agatgagttt ttaaccggac ttaagtctga aacagaagtc
    10921 gtagaaacat ctaaagactg caaatttgtt agatattgaa taccctctaa tgaagatatc
    10981 gttttcatat catgcaagtt taagaattgg gcttttgata tttcatcaac agataacaca
    11041 ctatcgccgt ctttatcaaa ttctgcaatg atttgttctc taaatacttt gtctggaaaa
    11101 tgtgcttcat caatagcaat gcttccagct tttacttctg tatttttgct tgcttcaacg
    11161 gtcttatctt cggtaaccac ttctttttct ggtgttgcag gtgtttcctg ttttgtactt
    11221 tcttctttta aagtttctgc attttcagca gtttcttcct gttccttgtc tgttatattc
    11281 tcttgtactt ctgcgttttc tgttttctgt tccacaggaa cttgctctgt cttgttttct
    11341 tctacaacgg cttcctgcac aacttctgtg ttttcctgtg ccatgacagg agtaggaagc
    11401 cctgtaagtg cagttgttgt tccaatcatc aatgcggaac ttactgcaag gacttgtttt
    11461 ttcttcatag tttgttcact ccttatttt
//
LOCUS       DAEOSP010000427         9149 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.guided.156, whole genome shotgun sequence.
ACCESSION   DAEOSP010000427 DAEOSP010000000
VERSION     DAEOSP010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9149
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.guided.156"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            81..197
                     /locus_tag="KRH54_003675"
     CDS             81..197
                     /locus_tag="KRH54_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBG8581274.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            213..2132
                     /gene="tet(M)"
                     /locus_tag="KRH54_003676"
     CDS             213..2132
                     /gene="tet(M)"
                     /locus_tag="KRH54_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBG8581275.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2251..2418
                     /locus_tag="KRH54_003677"
     CDS             2251..2418
                     /locus_tag="KRH54_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBG8581276.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2478..2831)
                     /locus_tag="KRH54_003678"
     CDS             complement(2478..2831)
                     /locus_tag="KRH54_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8581277.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            3336..3758
                     /locus_tag="KRH54_003679"
     CDS             3336..3758
                     /locus_tag="KRH54_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG8581278.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            3755..3985
                     /locus_tag="KRH54_003680"
     CDS             3755..3985
                     /locus_tag="KRH54_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8581279.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            4446..4649
                     /locus_tag="KRH54_003681"
     CDS             4446..4649
                     /locus_tag="KRH54_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBG8581280.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            4731..5948
                     /locus_tag="KRH54_003682"
     CDS             4731..5948
                     /locus_tag="KRH54_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBG8581281.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(6132..7034)
                     /locus_tag="KRH54_003683"
     CDS             complement(6132..7034)
                     /locus_tag="KRH54_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBG8581282.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            complement(7084..7545)
                     /locus_tag="KRH54_003684"
     CDS             complement(7084..7545)
                     /locus_tag="KRH54_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBG8581283.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            complement(7597..8052)
                     /gene="def"
                     /locus_tag="KRH54_003685"
     CDS             complement(7597..8052)
                     /gene="def"
                     /locus_tag="KRH54_003685"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBG8581284.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIEQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(8024..8287)
                     /locus_tag="KRH54_003686"
     CDS             complement(8024..8287)
                     /locus_tag="KRH54_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBG8581285.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(8384..9100)
                     /locus_tag="KRH54_003687"
     CDS             complement(8384..9100)
                     /locus_tag="KRH54_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBG8581286.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISLLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
BASE COUNT         3166 a         1492 c         1493 g         2998 t
ORIGIN      
        1 tataattgac agatagataa gtcaattaca atataccagg gcttagtttt ttgtacccag
       61 tttaagaata cctttatcat gtgattctaa agtatccaga gaatatctgt atgctttgta
      121 tacctatggt tatgcataaa aatcccagtg ataaaagtat ttatcactgg gatttttatg
      181 cccttttggg tttttgaatg gaggaaaatc acatgaaaat tattaatatt ggagttttag
      241 ctcatgttga tgcaggaaaa actaccttaa cagaaagctt attatataac agtggagcga
      301 ttacagaatt aggaagcgtg gacaaaggta caacgaggac ggataatacg cttttagaac
      361 gtcagagagg aattacaatt cagacaggaa taacctcttt tcagtgggaa aatacgaagg
      421 tgaacatcat agacacgcca ggacatatgg atttcttagc agaagtatat cgttcattat
      481 cagttttaga tggggcaatt ctactgattt ctgcaaaaga tggcgtacaa gcacaaactc
      541 gtatattatt tcatgcactt aggaaaatgg ggattcccac aatctttttt atcaataaga
      601 ttgaccaaaa tggaattgat ttatcaacgg tttatcagga tattaaagag aaactttctg
      661 ccgaaattgt aatcaaacag aaggtagaac tgtatcctaa tatgtgtgtg acgaacttta
      721 ccgaatctga acaatgggat acggtaatag agggaaacga tgacctttta gagaaatata
      781 tgtccggtaa atcattagaa gcattggaac tcgaacaaga ggaaagcata agatttcata
      841 attgttccct gttccctgtt tatcacggaa gtgcaaaaaa caatataggg attgataacc
      901 ttatagaagt gattacgaat aaattttatt catcaacaca tcgaggtccg tctgaacttt
      961 gcggaaatgt tttcaaaatt gaatatacaa aaaaaagaca acgtcttgca tatatacgcc
     1021 tttatagtgg agtactacat ttacgagatt cggttagagt atcagaaaaa gaaaaaataa
     1081 aagttacaga aatgtatact tcaataaatg gtgaattatg taagattgat agagcttatt
     1141 ctggagaaat tgttattttg caaaatgagt ttttgaagtt aaatagtgtt cttggagata
     1201 caaaactatt gccacagaga aaaaagattg aaaatccgca ccctctacta caaacaactg
     1261 ttgaaccgag taaacctgaa cagagagaaa tgttgcttga tgcccttttg gaaatctcag
     1321 atagtgatcc gcttctacga tattacgtgg attctacgac acatgaaatt atactttctt
     1381 tcttagggaa agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg
     1441 agatagaact aaaagagcct acagtcattt atatggagag accgttaaaa aatgcagaat
     1501 ataccattca catcgaagtg ccgccaaatc ctttctgggc ttccattggt ttatctgtat
     1561 caccgcttcc gttgggaagt ggaatgcagt atgagagctc ggtttctctt ggatacttaa
     1621 atcaatcatt tcaaaatgca gttatggaag ggatacgcta tggttgcgaa caaggattat
     1681 atggttggaa tgtgacggat tgtaaaatct gttttaagta tggcttatac tatagccctg
     1741 ttagtacccc agcagatttt cggatgcttg ctcctattgt attggaacaa gtcttaaaaa
     1801 aagctggaac agaattgtta gagccatatc ttagttttaa aatttatgcg ccacaggaat
     1861 atctttcacg agcatacaac gatgctccta aatattgtgc gaacatcgta gacactcaat
     1921 tgaaaaataa tgaggtcatt cttagtggag aaatccctgc tcggtgtatt caagaatatc
     1981 gtagtgattt aactttcttt acaaatggac gtagtgtttg tttaacagag ttaaaagggt
     2041 accatgttac taccggtgaa cctgtttgcc agccccgtcg tccaaatagt cggatagata
     2101 aagtacgata tatgttcaat aaaataactt agtgtatttt atgttgttat ataaatatgg
     2161 tttcttgtta aataagatga aatatttttt aataaagatt tgaattaaag tgtaaaggag
     2221 gagatagtta ttataaacta caagtggata ttgtgtcctg tatgtggaaa taaaacacga
     2281 ttaaagataa gggaagatac tgaattaaaa aaattccccc tctattgtcc gaaatgcaga
     2341 caagaaaatt taattgaaat aaagcagttc aaagtaactg tgattacaga gccagacgca
     2401 aagacgcaga gccgataaaa tgagattaat acaatctcat tttatcggct ctttccgtta
     2461 tgtatggatt cttttaatta gtcttcgatg tttcttgctt cgttgatacc gctggctaaa
     2521 gattccatta aggatagttc tttgtctgta aagctatcca tgtatttctc tatctgtaat
     2581 cgtcgggtgc tttttaccaa gttattagca ggtaagaaaa attcatcaac ggaaacatga
     2641 agtaacgata caaggtcata aagaacttgt atgctggggt gttgcccttt attttcaata
     2701 ttagttaagt accgtgggtc aatttcaatc aatgctccca cttgttcacg agttaaacct
     2761 cgtttcaatc gagcttcttt aatggctaaa ccaaaggctc taaaatcata tttatcttct
     2821 tttttacgca tagtagacca cctctataca ttttattgtt cctactgaat taaaaacagg
     2881 tatagaaaaa cgtgttatat ggtttatagg tttatattta ataaaaagca ctactaaacg
     2941 ccaataaaaa aaaccgttat atggtagtgc tatttacgct gttaaaatat tgtatattac
     3001 ttccaaatgg cggtttgttg gaggtcaacg tcgccatgaa gtacatcata tacaataaat
     3061 ttccttacat tgggttcttg tcaaaaaaag tcgtctatct gcaatagata agtacgtcca
     3121 ccaatgtggt tttataaatc atatagatag aataacagaa gcatgtaaac agagaaataa
     3181 atctgtttat atgctttttt ggctattcag aactttttta caaagtttat ttatcagtaa
     3241 tgcaacaaat ccccctttca cattgggact aagagtgaaa ggagataaac gagcaaggct
     3301 cacttccttt cctagacaga aagggggtga gaaacatgaa accatcttct tttcagacca
     3361 caatagaaaa tcagtttgac tatatctgta aacgtgctat ggaagacgag cgaaagaatt
     3421 atatgcttta tctttcaagg attgcaaagc gtgaggtgtc cttttcggat gttggcgatt
     3481 atcttgttag ccagtttgcg acaacagata actattcaac tgactttcag atttttacac
     3541 tcaatgggtt atcagtaggc gttgaaaatg atttgttgag tgaagcatta cgtgagttgc
     3601 cagacaagaa acgtgaaatt ctactgctgt tttactttat ggacatgagc gattcagaaa
     3661 ttgcagacct gttgaaattg aaccgttcta ctgtctatcg gcatagaacc agtggactag
     3721 ccttaattaa aaagtttatg gaggaatttg aagaatgaaa acacaatatc ctatgattcc
     3781 ctttcctctc attgtaaagg caacagatgg cgataccgaa gcgattaacc agattctaca
     3841 tcattacaga gggtacataa cgaagcgttc cctacgactt atgaaagatg aatatggcaa
     3901 tcaaagtatg gtcgttgatg aagtcttacg tggaagaatg gaaaccagac tgattacaaa
     3961 gattttgtca tttgaaatta agtaatatcc tctctccttt cgtggaagcg tgctaaacca
     4021 ttccacgctt cccgaacagg gaggtttgtt attccaccaa agcatattga gctttcaatg
     4081 tgttttgata ggctaacgag ccattgttct ttgaaaactg aataaaagta atcgaatacg
     4141 tttcgataag aaaagagcca acggaactaa ccgccatgac ctatcttata aagatagcga
     4201 gcgattcatg ttagtgatcc gagaagcaat ctttagcagg attgcctgca acgacattct
     4261 tatcgtgata atgatactcc catacagtca atagtccgag cgtgataaaa ccgtcgcagg
     4321 caatgagtat ggctacatga gaaccatgca ggggtggaac tcccgtgagc tttgctaaag
     4381 ctgttcgatt gctggtaaaa caacttttat gaaatccaaa taagtgattt ggaaaggagg
     4441 attttatgaa gcagactgac attcctattt gggaacgtta taccctaacc attgaagaag
     4501 cgtcaaaata ttttcgtatt ggcgaaaaca agctacgacg cttggcagag gaaaataaaa
     4561 atgcaaattg gctgattatg aatggcaatc gtattcagat taaacgaaaa caatttgaaa
     4621 aaattataga tacattggac gcaatctagc gtcgccaaag ggtcttgtat atgataaaat
     4681 agtattaagt cgtatcaagg ctctttccat aaaggaaagg agcaaatgcc atgtcagaaa
     4741 aaagacgtga caataaaggt cgaatcttaa agactggaga gagccaacga aaagacggaa
     4801 gatacttata caaatatata gattcatttg gagaaccgca atttgtttac tcgtggaaac
     4861 ttgtggctac agaccgagta ccagcaggaa agcgtgattg tatctcactt agagagaaaa
     4921 tcgcagagtt acagaaagac attcatgatg gtattgatgt tgtaggaaag aaaatgacac
     4981 tctgccagct ttacgcaaaa cagaacgctc aaagaccaaa ggttagaaaa aacactgaaa
     5041 ctggacgcaa atatcttatg gatattttga agaaagacaa gttaggtgta agaagtattg
     5101 acagtattaa gccatcagac gctaaagaat gggctattag aatgagtgaa aatggttatg
     5161 cttatcaaac catcaataac tacaaacgtt ctttaaaggc ttcattctat attgctatac
     5221 aagatgattg tgttcggaag aatccatttg actttcaact gaaagcagtt cttgatgatg
     5281 atactgtccc taagaccgta ctaacagaag aacaggaaga aaaactgtta gcctttgcaa
     5341 aagctgataa aacctacagc aaaaattatg atgaaattct gatactctta aaaacaggtc
     5401 ttcgtatttc agagtttggt ggtttgacac ttccagattt agattttgag aatcgtcttg
     5461 tcaatataga ccatcagcta ttgagagata ctgaaattgg gtactacatt gaaacaccaa
     5521 agaccaaaag tggcgaacgt caagttccta tggttgaaga agcctatcaa gcatttaagc
     5581 gagtgttagc gaatcgaaag aatgataagc gtgttgagat tgatggatat agtgatttcc
     5641 tctttcttaa tagaaagaac tatccaaaag tggcaagtga ttacaacggc atgatgaaag
     5701 gtcttgttaa gaaatacaat aagtataacg aggataaatt gccacacatc actccacata
     5761 gtttgcgaca tacattctgt accaactatg caaatgcagg aatgaatcca aaggcattac
     5821 agtacattat gggacatgct aatatagcca tgacgctgaa ctattacgca catgcaacat
     5881 tcgattctgc aatggcagaa atgaaacgct tgaataaaga gaagcaacag gagcgtcttg
     5941 ttgcttagta gtacaaatga atttactact tatttaccac ttctgacagc taagacatga
     6001 ggaaatatgc aaagaaacgt gaagtatctt cctacagtaa aaatactcga aagcacatag
     6061 aataaggctt tacgagcatt taagaaaata taaaaagata attagaaatt tatactttgt
     6121 tttatataaa actaatcaaa aaaagatatc tgtacatctc ctgagttttt taacttttcc
     6181 ttcattattt tagagtaact gcttgataga gaatacttat atctaagttc attaaccatt
     6241 ttatacaatt gatttttata ctccctatta agtgaacctg cactatatag tattgataat
     6301 ttatcaaata attcaggaaa ctctttttta acaaagtcaa aaaaaacacc tctagttata
     6361 ccccttaagt ataaagtccc aggtaaaaca taatgtacat tacattcttt tgcatgtcta
     6421 aatagcgaat ttatattatc aaaatcatct gtaatatatg gtataattgg cataatatgt
     6481 agaccaactg atgcgttagt ttttctaaat tcttttaaca ttttaaatcg tctcattgaa
     6541 tccacaccat ttggctcaat aagcctttgt gtatcttcat ttactgtggt aattgtagat
     6601 gctacattga tatatgtaat cctagatagc ttatcaatta aatcgtaatc tcttaaaatt
     6661 aaatctgact ttgtagaaat aatagctggt gtcttgtatt ttattagcag gtttaatatt
     6721 tctggcataa ttttatagtc agcttctatt ggttgataac tatctgtaac tcctcctata
     6781 ttgattactt ctttcttcca tttactgctt ttaagttgtc tctcaagttt ttctactata
     6841 tttgttttta cataaatctc ttcaaaataa ttattggagt tgatgtactt atgagagtaa
     6901 atagcataac aatatttaca tccatgctca catccacgat aaatatttaa atcccattta
     6961 tatggcatac tcctttttaa tttattacaa gctatttcac aatttattgg aatatatttt
     7021 attttattat acaaataatc acctctttat atacaataat actataatgt taatctatag
     7081 taattaaaac atgatatttc cagaccacaa ttcataatct ttactatatt tacaaagtaa
     7141 tatattccat tcatttattg gtattcctcc acatgttgct ttttcttcta aattatataa
     7201 gcattgcttt aaatacattt tattatgcca atcatcatat aaattttcta aatttaaact
     7261 atgttcatta gatatatcat ttgcaaaatt tctatttttc cattttaata tacttgtata
     7321 tttaagttca ttaggtttat aatttctttt taacatttca tcatgtacaa tttttgtata
     7381 aaatttaaat tcatctatac tataattcaa tactttattc actaatagat ggtttggagt
     7441 acctttttta tctatagaac cttttatagc aagtagctct ctccattgac ttactaattg
     7501 atttttaggt aatacatcaa ttaaatcctt atgccataat ctcataattt aactccttac
     7561 tataaaatat tttataaatt caataaccaa tctatcttac aattttacat gttcaataat
     7621 tttatctgta aaaactatac catttaaatg gtctatctca tgacaaagac attttgccat
     7681 aaaacctgaa cctttaattt ctattttctc accgtattcg tttagagctt gcactgtaac
     7741 tttttttggt ctttttaatt taccccatac ttcaggaaaa cttaaacatc cttcaacaac
     7801 aatttgttcc ccttcttgtt caataatttc aggattaaca agcttaataa gtccttctcc
     7861 taaatctata acaactaatc gctttaatac tcctacttgg catgcagcta atcctcctcc
     7921 atttggagta ttgtacatgg tctctgccat gtcatttaat atttctctta tcttattatc
     7981 tacgttttct acatacttac tcttttttct taaaatttca tcatcaaatg ttcttatctt
     8041 tcttatagcc ataatattct cccctactta tctgtgctct tgattttctt tcactttctt
     8101 caaataaaaa tacactttca attggttcat taaaaattct tgaaatatca tatgccaacc
     8161 atattgaagg ttcattttta ccagtttcta tagcatttat agcctgtcta gatgttccaa
     8221 ctaatctccc tagttgttct tgtgtcaatc ctaatttttc tcttaatatc tttattttat
     8281 ttttcatatt tgctccattc atgtaaggtt aactttacat cttaaatgta acatttttca
     8341 acattactgt caagtcaacc ttacatttat atattaaatt ttcttattta actatttttt
     8401 tactaaactt tttaaataat aataaaatcc atattaatga tataacaatt gaaataaaca
     8461 tatagacata attatcttcc ttaaatgcct tcgatatcca aaaagttggt aagaagaaca
     8521 gaatatattg tgctttattt aaaataaaaa atggtgctaa aatacccatc ataaaaacac
     8581 ctgagaattt cgtaattacc attccttcta gtttatttga tgataaagaa atcactaata
     8641 ttgatataat aactccttgt aataaaacta ataaagaaat tgataagttt aacaagagag
     8701 aaattttagt tagagaaaaa aagataagta gaatcatagt aataataaat gctaagatac
     8761 ctggtattcc aaatcgtgaa ataagataac cagttttacc aagcggagtt actattaaat
     8821 atcttgaaat gctgtcatca atttctccca atatgaccat tgtagaagca aaaaagaaca
     8881 taaaaggtgt aataaaagct aaaagtaaat caaacatcaa gaaatacggc tctaaattta
     8941 attgataata aaacttgtta agtgatatat tctgtattaa tggtattcca aattttataa
     9001 atactccaca taatattgga gaaacacata ctataaaaag catagcatct ctcttaattt
     9061 gttcaaactc ttgtttaaac gcatttataa tctttatcat agttttactc cacctacctt
     9121 tttccaactg ctacaaataa aataataag
//
LOCUS       DAEOSP010000428         8195 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.guided.166, whole genome shotgun sequence.
ACCESSION   DAEOSP010000428 DAEOSP010000000
VERSION     DAEOSP010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.guided.166"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            complement(605..1420)
                     /locus_tag="KRH54_003688"
                     /pseudo
     CDS             complement(605..1420)
                     /locus_tag="KRH54_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1512..2180
                     /locus_tag="KRH54_003689"
                     /pseudo
     CDS             1512..2180
                     /locus_tag="KRH54_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            2307..2927
                     /locus_tag="KRH54_003690"
     CDS             2307..2927
                     /locus_tag="KRH54_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBG8581287.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            3083..3865
                     /locus_tag="KRH54_003691"
     CDS             3083..3865
                     /locus_tag="KRH54_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBG8581288.1"
                     /translation="MTKTQQEMIQNVLEFSKRDSRIIGLAISGSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYTCTKIGRR
                     ELFETIDFLSFLRQSVLASLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            3990..4916
                     /locus_tag="KRH54_003692"
     CDS             3990..4916
                     /locus_tag="KRH54_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBG8581289.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            4930..5368
                     /locus_tag="KRH54_003693"
                     /pseudo
     CDS             4930..5368
                     /locus_tag="KRH54_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            5729..6136
                     /locus_tag="KRH54_003694"
     CDS             5729..6136
                     /locus_tag="KRH54_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBG8581290.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            6138..6368
                     /locus_tag="KRH54_003695"
     CDS             6138..6368
                     /locus_tag="KRH54_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBG8581291.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            6758..6961
                     /locus_tag="KRH54_003696"
                     /pseudo
     CDS             6758..6961
                     /locus_tag="KRH54_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
     gene            7039..7392
                     /locus_tag="KRH54_003697"
     CDS             7039..7392
                     /locus_tag="KRH54_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBG8581292.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKAQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNVLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            7464..8096
                     /locus_tag="KRH54_003698"
     CDS             7464..8096
                     /locus_tag="KRH54_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBG8581293.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         2928 a         1192 c         1379 g         2696 t
ORIGIN      
        1 attaaaaata ctatacatta taaaaatact aatttaaaaa taatttttat tctaattctt
       61 aaaacaatcc cacatatttt agaaaaggta ctctataata tacaaacact tttttttacc
      121 taaatttatt ttaacattct ataattttca caatattctt taaattttac tcatataaac
      181 aatattattc tggatgctaa tagaattaat ctataaatca tagtataatt atgcagaaat
      241 aactcgaatt tcactactta tgaagagatt gaatattaat gaatctaaat catttgcatt
      301 atttaagagt tttgactaaa gttgaacacc atacaaaagt agctactcaa ttgtttatac
      361 cccaaccaag cttaagtaat gttatataga ttctctaaaa agaatttagt atctttttat
      421 ttgaaaaact tatattaaat ttgattatct tttcataagt tggtaaatgt cgatatatcc
      481 tgtatctaca aggagtatcg acattttctt tttggaagat actttgcata agttggcatt
      541 taaccgtata agaatgccat caaaagtagt aaataagtag taaactctag tcatactact
      601 aggattataa ccgttccatt tcagctctcg cagaactaaa tgaaccatgt gcataatagc
      661 caagcgtcat agtaatgttt ttatgcccca taatatattg caatatatta gtactcattc
      721 ctttgttagc catatttgta cagagtgtat gcctaaaggt atgtggcgta atctttggta
      781 gcttgtcctc atgggtcttg ttatacttct tgactaacgc tcttaacatg ctttgataat
      841 gagttgcttg ttttggtaat ccatcttgtt taagaaatag gaagttctgg taaccatcaa
      901 caatgattgg ttctgcttta gcgttcttac gccgttcaac gatacgttca aatgattgat
      961 aagctctttc agtcattgga atttctcgct taccctgtgt ggtctttgga acttctacat
     1021 aatagccaat cttagtatca ctcaacaatt gatggtctac cataatggct ttatttttca
     1081 tatctaattc taaagtcaaa ccgcataatt cagaaatgcg tagtcctgtt tctaagagga
     1141 taacaacatc atcataatat ttataataag tcttatcgct ccccataaag gataataagc
     1201 ccatttcctg ttctggtgtt agagcgattt ttggttctgt tgtatcttcc aaaacatcac
     1261 ttaactaaaa attaaatgga ttctttctaa tacagtcgtc ttgaattgcc atgaaaaacg
     1321 acgctttcag tgagcgctta taattgtcaa tggatttata agcacaccca tcttcactca
     1381 ttcggatagc ccagctttta gcgtctgata atttaaccat atcaatgctt tttgcaccta
     1441 gtgggtcatt gatagtatct atattttgat ttataattta tacatttttt taaaaagaga
     1501 cttttaactg taaagaatct atgtataaac ttagctatgt tgtccgtgta tctacaaagg
     1561 atcttgacga gctaaaaagg cgttgtgatg aagtcaaaga cttttacgac gacttatcaa
     1621 tcaagctcgt gcgtcccttt ggggatatgt tgggattgca tggcgaattt attcctgtca
     1681 gcaaacgtta catcaatgat tatattcaat atgtcacttc ggattttttg gctggacttg
     1741 gttttggagc gacacaaacc cttggcgaac atgaggatat ctatcttggc tacaatctgg
     1801 acactggaaa gaatgtctat ctaaaacctg cccttgctag tcaaggggta aagggttcta
     1861 ttaccaatgc gttagcgtct gcgtttatcg gttcattggg tggtggtaaa ttattcagta
     1921 acaatatgct tgtttactat gcggtactat tcggcggaca agcggtcata gttgacccta
     1981 aagcaaaacg cggcaaatgg aaagaaacgc taccagaaat agcccatgag ataaacattg
     2041 tcaatttaac cagtgatgat gaaaacaaag gattgcttga ccattacgtc attttgagcc
     2101 gtaaaaagga ttctgaaagt ttagcgattg atatcctaac attccttact ggtatctcca
     2161 gtcgtgacag tgataagttt ttgcagaaaa atgacaactg gaaaataata atggaatagt
     2221 ttctatgtaa atatattttt actatcttac ctaactaatt gaaagatgat ataatttaat
     2281 caaagatttg gtaaggagag atgaatatgc atttaattga ttttatgaat gaagatatta
     2341 aaaagaagct tgttaaaaaa acgtataaaa agaaagagac tattcttttt gctgagcaag
     2401 aaaatgaata tgtaattctt atgattgacg gtattgcaga agcattcatc ttaaatgtga
     2461 aaggaaacat ctcaactatt catctttata gaagtggtag tttttttgga gaaatggaac
     2521 aatttaatga tgggaaaaaa cccgttgaaa ttgtcgcttt cacagattgt aatgtatata
     2581 aacttcatag agatgatttt ttaaactggt taaggggaga ttttgaagct acaaaatttc
     2641 tgattcgaga aatatctagt aaattagttg taaatgctga attaattgaa gaattatcat
     2701 ctttaagtgt taaagaacga cttctgaggt gtatatcctt acactattat agaagtgaat
     2761 taaatctgct taccaaaaaa cagcttgcta gcgaagtaaa cgctccgatt agaagcatta
     2821 atcgtgctat aaatgaatgt gtacaagaac atctaattgc ttatgaaaac aaaagattca
     2881 gtgtaattaa tcagcgagag gtattaaaga atcttcctac aacataagta taatttcaaa
     2941 aaagtattgg ataataatta ttcaatactt ttttatttcg ggtcaagtga cctatttttt
     3001 tttatttctt cattatataa tactcctatg cataattatg ttggattgat agctaataaa
     3061 agacagtgag aggtgttata ttatgactaa aacacaacag gaaatgatac aaaacgtatt
     3121 agaattttca aaaagagata gtaggataat aggacttgca attagcggtt cttatatcac
     3181 aaaatcgtta gatgaatatt ctgatttgga ttttctgatt gtagcagatg ataaatccta
     3241 tgaagaagtt atcgaagaga gattacttat tgtggaacag tttggaacat tagtttctgc
     3301 ttttactggc gaacatgttg gagaacctcg attgataatt tccctctatg attcccctgt
     3361 tcttcatgtt gattttaagt ttacaacctt agatggttta ctagataggg ttgaagattc
     3421 agctattctc tatgaagaaa acaactgcat aacagaaatt tattctaaaa aagaagcata
     3481 cttcccaact ccagacttac agtggattga agatagattt tggatttggg ttcattacac
     3541 ttgcactaag attggacgta gagaactatt tgaaacaatt gattttttat cttttttaag
     3601 acaatcagta cttgcctcgt taatacagct tctcttaggt aaattacctc gtggagttag
     3661 aaaaattgaa ttggacgcac cccaatatat tgaagcatta gaaaagacgg ttgctaacca
     3721 tgaatataaa agctgtgtta atgcattgca ggctactatc aacctgtatc ttgaattaag
     3781 agaggaatta tcacaaagtc ctattgtccg aaaggttgaa gctgaaaggg ttgcattaca
     3841 gtattttcaa gatttaatca aataaaccca aagtaaaaaa atattgaata gtaaatattc
     3901 agtgtttttt catttttggt caaatgacct agattagttg ttgtaacaat tctataatta
     3961 ttttgaaagt aacagtatgg aggataacta tgagtgatat tttaagtaga attacaaata
     4021 aaattgggga taagaatata gttgataagt tatcagctct atcaaaatca gatttgaact
     4081 cacttctatt agaagtgttt gatagacaag caaatacttt aactgcaact gatattctaa
     4141 agtcatacca actaaataga tttactatcc ctagcggtat agatccagaa gaaattcatg
     4201 ctttggagtc aaaattattg agaaaagcat tcaatatgga tatcaagacg attatgcttt
     4261 ctccctctgc tccattaggt agctgtagcg tttttggctc cgtagatcaa tataatgtag
     4321 taagtgctct acgagggaca gaaacacttg cagacccctc aaatatgttg gcaataatta
     4381 ttgctgataa attaaaaaga aaagaagaaa ataataccat acctatccac atggctacta
     4441 ctgctagggt aataagagca caaaattttg taggtaaagg tttatattca cattttggct
     4501 tatattgtat ggtttcatct ggtatagata cagggtctta cacttgtgaa aaaatgttgt
     4561 tagaaaagca cttaaactat tataaagata tattaagtga aatagaaaat gttcgtttct
     4621 ctattattct acgcaaaaga aacggataca aagataatga cggatttttc gacagaatgg
     4681 ttgaaactat caaacttatt tttcctaata tagagttatc aattcataat gatgatgttg
     4741 ataataatta ttataaaggt atcaatttca aattatcggt caaacaagga aatgaaaccg
     4801 ttgaaatagt tgacggtgga tttgttgact ggacttatca aatgcttgga aataaaaaag
     4861 aacgctgttt gataagtggt attggcttag aaagatttct tgtagctgtt tcataaaagg
     4921 agaagaaaaa tggaacttat agagtggaat tatgcttata ttaccgattt agttgagttt
     4981 gcgaatgata aagaaattgc aaacaatctt cgtgacaact tcccccaccc ttataggtag
     5041 caaaacgcta gagattttat tagtttttgt ttgacaactt cagatgttaa acaacttagc
     5101 tatgctattt tttatcaaga aagggctatt ggaagtatca gcctaacatt tgaagaagat
     5161 acctctatta ataaaaatgc tgaattaggt tactgacttg ctaggaacta ttggggcaaa
     5221 gggatagcaa caaatgcagt aaaagaaata tgcaaaattg cctttgaaaa atacaacctt
     5281 aacaaaattc atgctaatgt atattcctac aatagagagt caagtaaaat gtggctttaa
     5341 gattgaagaa tactgtcaca attcataata aagaatagaa acatgattga ttgccatgta
     5401 tatagtttaa ttcgaccgca atagcattta ttagaattaa cctacttttt aataacagaa
     5461 taagtttttc ccctctatta atttcttatg catctttgta tttttatgaa aagctagcca
     5521 atgcatattt ccatacatca atatggtggt ggcttacaac caatataaat taaatacgtt
     5581 ttaaagaata cacatttttg ccctataggc agagtgtgta tttttttctc tttttttttg
     5641 aaatttatcg aagatttaac gccctcccac cgtgatagga gtaagcaagg ttatttaact
     5701 ccttgcgatg gaaagagggt gaaatcttat ggttgatgaa aagacaattg aacatcaatt
     5761 cgacagtttc tgtaaaacag ttttgcgtaa ctatgctaga gatatttatg acgaaaataa
     5821 acgcagaaat gattatttgg tatctctcga atctttgtca cttgcagaat taagtaaatt
     5881 gagtatttta gatgattatg attctaacta catctgtatg gtttcttacg actacaacat
     5941 acgaattgaa gatgtattaa tggcacaagc aatcggaaaa cttacaaaaa gaaaacaaga
     6001 tataattcta ctttcattct ttctcaacat gacaaatgct gacatagcaa cattgatgga
     6061 tttagcagaa aataccatac attatcacaa aacaaatgca ctaaaagagt tgaaagaact
     6121 catgcaggaa cattgaaatg aaacctacta aagaagtatt accttttaca attattttac
     6181 ttgctgtaca tggcgatgta aatgcaataa acgtaatact taaacatttt gaacactata
     6241 tcattcgtct ttcacagaaa actttatttg atgaatacgg aaatccctat attcatgtag
     6301 aagaagaaac aaagcgaatg ctaggaacaa aactaatcac tgctattttg agatttaatt
     6361 tgtcttaatc tcgccctctc tccatgtatc aagctatttg gtacatggag aatcactaat
     6421 tatttgtcta taaaagccca taagctattg tgatttttta taggcaaatc aatgccgact
     6481 gttctttgac aactgaataa agtagataga tacgtttgtg atacagatag ccaacaaata
     6541 ctatacgcca tgaccttaat caagagagct actatatagt gatttgtgaa taacctttgg
     6601 aacggttgtt ttgccgtaac ctgtaccaca gtataatgat acacctgtat gacacggttc
     6661 tgcccataag aatgggattg gttgaaatac caatggagct taccaaagcc atctatctac
     6721 ttctgaacta cgaacataga aaggagtgat acattctatg aacaatactg atgtacctat
     6781 ttgggaaaaa tataccttga cgattgaata agcgtcaaaa tactttcgta ttggagagaa
     6841 aaaattacgt aaacttgctg atgaaaacct tgattctggc tgggtcatta tgaatagcaa
     6901 tcgcattcaa ataaagagaa aacaatttga gaaaatcgtt gatacacttg acgttatcta
     6961 atgaaattga gccttatatg ttataatatt catatgacgt atcaagctca attcggttat
     7021 tgagaggagc ttcttatcat gtcaactgaa aaaagacgag ataataaaga tagaatttta
     7081 agaactggag agagccagag aaaagatgga agatacgcat ataagtatgt atattcattt
     7141 ggtaaagcac aatttgttta ctcttggaaa cttgtaccta ctgataaacc cccaaaggga
     7201 aaacgtgatg atatatcatt aagggataag gaaaaagcaa tacaaaatga ccttgatgat
     7261 agcattgaca ctattggtaa gaaaatgaca gtttgtcagt tatacgctaa aaagaatgta
     7321 ctaagaaaga atatccgact taacactaaa aaaggacgcc attatcttat gaagattctt
     7381 aacaaggagt gattctgtga agagatgtat tgtagtagtg gaatagtcaa aaacataata
     7441 tatattaaag gagagattta aatatgacta ttccaagttc aaataaggta tatccaagaa
     7501 gtaatgatta tcaaactgta tatcttaaaa atgtaataac aagggaaaat atagaagttg
     7561 gagattacac aatatataat gacttttata atgacccaag agattttgag aaaaacaatg
     7621 tattgtatca ttaccctata aacaatgata aattaataat tggtaagttt tgctcaatag
     7681 catgtaatgc aaaatttcta atgactagtg gaaatcactc aatgaaatca aaatccacat
     7741 atacttttcc catcttttat gaagaatgga aactcgatat caaccatata acggacgctt
     7801 gggacaataa gggtaacatt ataattggaa atgatgtgtg gataggttat gattctataa
     7861 ttatgtcagg tgtaaatatt ggtgatggtg caattattgg aacaagagca gtagttacta
     7921 atgacgttcc accgtattct attgttggag gagtacctgc aaaaattata aaaaagagat
     7981 ttaataatga tacaatttca aaattattag aaattaaatg gtgggattgg ccatataaaa
     8041 aaattcaatc aaacattaag catattcaat caggaaatat tgataaatta gtttaatatt
     8101 ttataattta tttatattaa actttacttt aataatcatt ttataagagc tttttcaaaa
     8161 tttgtatatt attttaatat atataaattt aatac
//
LOCUS       DAEOSP010000429         1406 bp    DNA     linear   BCT 16-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile isolate CD-15-01004
            SAMEA5849386-rid12486793.guided.346, whole genome shotgun sequence.
ACCESSION   DAEOSP010000429 DAEOSP010000000
VERSION     DAEOSP010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5849386
            Sequence Read Archive: ERR3473840
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001070388.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001070388.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/16/2021 00:38:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,699
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,607
            CDSs (with protein)               :: 3,607
            Genes (RNA)                       :: 44
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 22 of 48
            Pseudo Genes (incomplete)         :: 18 of 48
            Pseudo Genes (internal stop)      :: 19 of 48
            Pseudo Genes (multiple problems)  :: 10 of 48
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5849386-rid12486793.guided.346"
                     /isolate="CD-15-01004"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Germany"
     gene            278..1231
                     /gene="blaCDD"
                     /locus_tag="KRH54_003699"
     CDS             278..1231
                     /gene="blaCDD"
                     /locus_tag="KRH54_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBG8581294.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYLGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEDISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLINKVDAKYVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT          601 a          112 c          231 g          462 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaatgt ggaatcaatt attatatcac
       61 aaattaaaag gaggacagga tgttattaat agttacctaa ttatcttcgc aataggttta
      121 atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca tttagtcttg
      181 tttcttataa gtagaaccta gattggagta tattacaaat aatgtatact attttaatag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatttag ggaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa ataagtctga
      421 attaaataaa tccaaaattg aaaacaatga taaaaacaaa aaagaaaaaa ttaatatggt
      481 tgactatagt gattgttttg aagatattag tggaggagct attttttata atactaaaaa
      541 taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac catgttctac
      601 ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaatt caaaagaatc
      661 agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa atcttagttt
      721 agaagaggca tttaaggagt cttgtgtttg gtactacaaa aaattgataa ataaagttga
      781 tgctaaatat gtccaaaaca ttttagatga tttaaagtat gggaattgcg atattagcga
      841 atgggaagga gatttaaaaa atggaaaggg acatttaaat ggattttggt tagaatcttc
      901 attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg agggagatac
      961 aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag atgtaaatga
     1021 taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa ataaacgtgt
     1081 ggatgcatgg tttgttggta tgctagagcg tgaaggtgac acctattatt ttgctattaa
     1141 atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg caattaatat
     1201 aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa tattatttac
     1261 ctatagtctg taatatttat taaataattt tactaaattg agtctatata gagcaaaact
     1321 cctttagtac ataaaatact tgctattgat tattaaattg aatacctata gatttaaagt
     1381 taataccttt taaagactat gtaata
//
